LOCUS       DACADA010000001        87343 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.001, whole genome shotgun sequence.
ACCESSION   DACADA010000001 DACADA010000000
VERSION     DACADA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.001"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(131..1531)
                     /gene="asnS"
                     /locus_tag="ISB17_000001"
     CDS             complement(131..1531)
                     /gene="asnS"
                     /locus_tag="ISB17_000001"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP1977993.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1700..2902)
                     /gene="pncB"
                     /locus_tag="ISB17_000002"
     CDS             complement(1700..2902)
                     /gene="pncB"
                     /locus_tag="ISB17_000002"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP1977994.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3168..5780
                     /gene="pepN"
                     /locus_tag="ISB17_000003"
     CDS             3168..5780
                     /gene="pepN"
                     /locus_tag="ISB17_000003"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAP1977995.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5823..6590)
                     /gene="ssuB"
                     /locus_tag="ISB17_000004"
     CDS             complement(5823..6590)
                     /gene="ssuB"
                     /locus_tag="ISB17_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1977996.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(6587..7378)
                     /gene="ssuC"
                     /locus_tag="ISB17_000005"
     CDS             complement(6587..7378)
                     /gene="ssuC"
                     /locus_tag="ISB17_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAP1977997.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7390..8535)
                     /gene="ssuD"
                     /locus_tag="ISB17_000006"
     CDS             complement(7390..8535)
                     /gene="ssuD"
                     /locus_tag="ISB17_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAP1977998.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8532..9491)
                     /locus_tag="ISB17_000007"
     CDS             complement(8532..9491)
                     /locus_tag="ISB17_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1977999.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9484..10059)
                     /gene="ssuE"
                     /locus_tag="ISB17_000008"
     CDS             complement(9484..10059)
                     /gene="ssuE"
                     /locus_tag="ISB17_000008"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP1978000.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10296..11306
                     /gene="pyrD"
                     /locus_tag="ISB17_000009"
     CDS             10296..11306
                     /gene="pyrD"
                     /locus_tag="ISB17_000009"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAP1978001.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11480..12022
                     /gene="zapC"
                     /locus_tag="ISB17_000010"
     CDS             11480..12022
                     /gene="zapC"
                     /locus_tag="ISB17_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAP1978002.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12019..13128)
                     /gene="ycbX"
                     /locus_tag="ISB17_000011"
     CDS             complement(12019..13128)
                     /gene="ycbX"
                     /locus_tag="ISB17_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAP1978003.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADGTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            13226..15334
                     /gene="rlmKL"
                     /locus_tag="ISB17_000012"
     CDS             13226..15334
                     /gene="rlmKL"
                     /locus_tag="ISB17_000012"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAP1978004.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15346..17253
                     /locus_tag="ISB17_000013"
     CDS             15346..17253
                     /locus_tag="ISB17_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1978005.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17383..18636
                     /gene="pqiA"
                     /locus_tag="ISB17_000014"
     CDS             17383..18636
                     /gene="pqiA"
                     /locus_tag="ISB17_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAP1978006.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18641..20281
                     /gene="pqiB"
                     /locus_tag="ISB17_000015"
     CDS             18641..20281
                     /gene="pqiB"
                     /locus_tag="ISB17_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAP1978007.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20278..20841
                     /gene="pqiC"
                     /locus_tag="ISB17_000016"
     CDS             20278..20841
                     /gene="pqiC"
                     /locus_tag="ISB17_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAP1978008.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            21097..21264
                     /gene="rmf"
                     /locus_tag="ISB17_000017"
     CDS             21097..21264
                     /gene="rmf"
                     /locus_tag="ISB17_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAP1978009.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21334..21852)
                     /gene="fabA"
                     /locus_tag="ISB17_000018"
     CDS             complement(21334..21852)
                     /gene="fabA"
                     /locus_tag="ISB17_000018"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAP1978010.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(21921..23681)
                     /locus_tag="ISB17_000019"
     CDS             complement(21921..23681)
                     /locus_tag="ISB17_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAP1978011.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            23867..24319
                     /gene="matP"
                     /locus_tag="ISB17_000020"
     CDS             23867..24319
                     /gene="matP"
                     /locus_tag="ISB17_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAP1978012.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24395..25447)
                     /gene="ompA"
                     /locus_tag="ISB17_000021"
     CDS             complement(24395..25447)
                     /gene="ompA"
                     /locus_tag="ISB17_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAP1978013.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(25803..26312)
                     /gene="sulA"
                     /locus_tag="ISB17_000022"
     CDS             complement(25803..26312)
                     /gene="sulA"
                     /locus_tag="ISB17_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAP1978014.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26531..27160
                     /gene="sxy"
                     /locus_tag="ISB17_000023"
     CDS             26531..27160
                     /gene="sxy"
                     /locus_tag="ISB17_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAP1978015.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27123..29285)
                     /gene="yccS"
                     /locus_tag="ISB17_000024"
     CDS             complement(27123..29285)
                     /gene="yccS"
                     /locus_tag="ISB17_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAP1978016.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29295..29741)
                     /locus_tag="ISB17_000025"
     CDS             complement(29295..29741)
                     /locus_tag="ISB17_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP1978017.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            29864..31918
                     /gene="helD"
                     /locus_tag="ISB17_000026"
     CDS             29864..31918
                     /gene="helD"
                     /locus_tag="ISB17_000026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAP1978018.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(31950..32408)
                     /gene="mgsA"
                     /locus_tag="ISB17_000027"
     CDS             complement(31950..32408)
                     /gene="mgsA"
                     /locus_tag="ISB17_000027"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP1978019.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32504..33166)
                     /locus_tag="ISB17_000028"
     CDS             complement(32504..33166)
                     /locus_tag="ISB17_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAP1978020.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33339..33752
                     /locus_tag="ISB17_000029"
     CDS             33339..33752
                     /locus_tag="ISB17_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP1978021.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(33797..34114)
                     /gene="hspQ"
                     /locus_tag="ISB17_000030"
     CDS             complement(33797..34114)
                     /gene="hspQ"
                     /locus_tag="ISB17_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAP1978022.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34172..35362)
                     /gene="rlmI"
                     /locus_tag="ISB17_000031"
     CDS             complement(34172..35362)
                     /gene="rlmI"
                     /locus_tag="ISB17_000031"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAP1978023.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35457..35735
                     /gene="yccX"
                     /locus_tag="ISB17_000032"
     CDS             35457..35735
                     /gene="yccX"
                     /locus_tag="ISB17_000032"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP1978024.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(35732..36061)
                     /gene="tusE"
                     /locus_tag="ISB17_000033"
     CDS             complement(35732..36061)
                     /gene="tusE"
                     /locus_tag="ISB17_000033"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAP1978025.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36153..36812)
                     /gene="yccA"
                     /locus_tag="ISB17_000034"
     CDS             complement(36153..36812)
                     /gene="yccA"
                     /locus_tag="ISB17_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAP1978026.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37021..37108)
                     /locus_tag="ISB17_000035"
     tRNA            complement(37021..37108)
                     /locus_tag="ISB17_000035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37072..37074),aa:Ser,seq:tga)
     gene            37536..38654
                     /gene="hyaA"
                     /locus_tag="ISB17_000036"
     CDS             37536..38654
                     /gene="hyaA"
                     /locus_tag="ISB17_000036"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAP1978027.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            38651..40444
                     /gene="hyaB"
                     /locus_tag="ISB17_000037"
     CDS             38651..40444
                     /gene="hyaB"
                     /locus_tag="ISB17_000037"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAP1978028.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQLIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            40463..41170
                     /gene="hyaC"
                     /locus_tag="ISB17_000038"
     CDS             40463..41170
                     /gene="hyaC"
                     /locus_tag="ISB17_000038"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAP1978029.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            41167..41754
                     /locus_tag="ISB17_000039"
     CDS             41167..41754
                     /locus_tag="ISB17_000039"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAP1978030.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQG"
     gene            41751..42149
                     /gene="hyaE"
                     /locus_tag="ISB17_000040"
     CDS             41751..42149
                     /gene="hyaE"
                     /locus_tag="ISB17_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAP1978031.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            42146..43003
                     /locus_tag="ISB17_000041"
     CDS             42146..43003
                     /locus_tag="ISB17_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAP1978032.1"
                     /translation="MSETFFHLLGPGTQLNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            43137..44681
                     /gene="appC"
                     /locus_tag="ISB17_000042"
     CDS             43137..44681
                     /gene="appC"
                     /locus_tag="ISB17_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAP1978033.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            44693..45829
                     /gene="appB"
                     /locus_tag="ISB17_000043"
     CDS             44693..45829
                     /gene="appB"
                     /locus_tag="ISB17_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP1978034.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            45842..45934
                     /gene="cbdX"
                     /locus_tag="ISB17_000044"
     CDS             45842..45934
                     /gene="cbdX"
                     /locus_tag="ISB17_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAP1978035.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            46014..47324
                     /locus_tag="ISB17_000045"
     CDS             46014..47324
                     /locus_tag="ISB17_000045"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAP1978036.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEVRIPACALHQDK"
     gene            complement(47312..47524)
                     /gene="cspH"
                     /locus_tag="ISB17_000046"
     CDS             complement(47312..47524)
                     /gene="cspH"
                     /locus_tag="ISB17_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAP1978037.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            47810..48022
                     /gene="cspG"
                     /locus_tag="ISB17_000047"
     CDS             47810..48022
                     /gene="cspG"
                     /locus_tag="ISB17_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAP1978038.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            48033..48221
                     /locus_tag="ISB17_000048"
     CDS             48033..48221
                     /locus_tag="ISB17_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP1978039.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            48196..48426
                     /gene="ymcE"
                     /locus_tag="ISB17_000049"
     CDS             48196..48426
                     /gene="ymcE"
                     /locus_tag="ISB17_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAP1978040.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            48416..48589
                     /locus_tag="ISB17_000050"
     CDS             48416..48589
                     /locus_tag="ISB17_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAP1978041.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(48638..49711)
                     /locus_tag="ISB17_000051"
     CDS             complement(48638..49711)
                     /locus_tag="ISB17_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAP1978042.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLAFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(49794..52526)
                     /gene="torS"
                     /locus_tag="ISB17_000052"
     CDS             complement(49794..52526)
                     /gene="torS"
                     /locus_tag="ISB17_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAP1978043.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRIDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRVPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            52609..53637
                     /gene="torT"
                     /locus_tag="ISB17_000053"
     CDS             52609..53637
                     /gene="torT"
                     /locus_tag="ISB17_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAP1978044.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(53610..54302)
                     /gene="torR"
                     /locus_tag="ISB17_000054"
     CDS             complement(53610..54302)
                     /gene="torR"
                     /locus_tag="ISB17_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAP1978045.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            54432..55604
                     /gene="torC"
                     /locus_tag="ISB17_000055"
     CDS             54432..55604
                     /gene="torC"
                     /locus_tag="ISB17_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAP1978046.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            55604..58150
                     /gene="torA"
                     /locus_tag="ISB17_000056"
     CDS             55604..58150
                     /gene="torA"
                     /locus_tag="ISB17_000056"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAP1978047.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            58147..58746
                     /gene="torD"
                     /locus_tag="ISB17_000057"
     CDS             58147..58746
                     /gene="torD"
                     /locus_tag="ISB17_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAP1978048.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(59001..59306)
                     /gene="cbpM"
                     /locus_tag="ISB17_000058"
     CDS             complement(59001..59306)
                     /gene="cbpM"
                     /locus_tag="ISB17_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAP1978049.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(59306..60226)
                     /gene="cbpA"
                     /locus_tag="ISB17_000059"
     CDS             complement(59306..60226)
                     /gene="cbpA"
                     /locus_tag="ISB17_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAP1978050.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            60761..62002
                     /gene="agp"
                     /locus_tag="ISB17_000060"
     CDS             60761..62002
                     /gene="agp"
                     /locus_tag="ISB17_000060"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAP1978051.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(62040..62267)
                     /locus_tag="ISB17_000061"
     CDS             complement(62040..62267)
                     /locus_tag="ISB17_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978052.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(62288..62884)
                     /gene="wrbA"
                     /locus_tag="ISB17_000062"
     CDS             complement(62288..62884)
                     /gene="wrbA"
                     /locus_tag="ISB17_000062"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP1978053.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            63257..63430
                     /locus_tag="ISB17_000063"
     CDS             63257..63430
                     /locus_tag="ISB17_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAP1978054.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(63513..64841)
                     /gene="rutG"
                     /locus_tag="ISB17_000064"
     CDS             complement(63513..64841)
                     /gene="rutG"
                     /locus_tag="ISB17_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAP1978055.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(64862..65356)
                     /gene="rutF"
                     /locus_tag="ISB17_000065"
     CDS             complement(64862..65356)
                     /gene="rutF"
                     /locus_tag="ISB17_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAP1978056.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(65367..65957)
                     /locus_tag="ISB17_000066"
     CDS             complement(65367..65957)
                     /locus_tag="ISB17_000066"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAP1978057.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(65967..66767)
                     /gene="rutD"
                     /locus_tag="ISB17_000067"
     CDS             complement(65967..66767)
                     /gene="rutD"
                     /locus_tag="ISB17_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAP1978058.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(66775..67161)
                     /gene="rutC"
                     /locus_tag="ISB17_000068"
     CDS             complement(66775..67161)
                     /gene="rutC"
                     /locus_tag="ISB17_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAP1978059.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(67173..67865)
                     /gene="rutB"
                     /locus_tag="ISB17_000069"
     CDS             complement(67173..67865)
                     /gene="rutB"
                     /locus_tag="ISB17_000069"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAP1978060.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(67865..68956)
                     /gene="rutA"
                     /locus_tag="ISB17_000070"
     CDS             complement(67865..68956)
                     /gene="rutA"
                     /locus_tag="ISB17_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAP1978061.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            69244..69882
                     /gene="rutR"
                     /locus_tag="ISB17_000071"
     CDS             69244..69882
                     /gene="rutR"
                     /locus_tag="ISB17_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAP1978062.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(69922..73884)
                     /gene="putA"
                     /locus_tag="ISB17_000072"
     CDS             complement(69922..73884)
                     /gene="putA"
                     /locus_tag="ISB17_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP1978063.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            73939..74148
                     /locus_tag="ISB17_000073"
     CDS             73939..74148
                     /locus_tag="ISB17_000073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978064.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            74307..75815
                     /gene="putP"
                     /locus_tag="ISB17_000074"
     CDS             74307..75815
                     /gene="putP"
                     /locus_tag="ISB17_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAP1978065.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            76035..76865
                     /locus_tag="ISB17_000075"
     CDS             76035..76865
                     /locus_tag="ISB17_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HAP1978066.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            76923..78050
                     /locus_tag="ISB17_000076"
     CDS             76923..78050
                     /locus_tag="ISB17_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAP1978067.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            78056..79327
                     /gene="efeB"
                     /locus_tag="ISB17_000077"
     CDS             78056..79327
                     /gene="efeB"
                     /locus_tag="ISB17_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAP1978068.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            79678..80742
                     /gene="phoH"
                     /locus_tag="ISB17_000078"
     CDS             79678..80742
                     /gene="phoH"
                     /locus_tag="ISB17_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAP1978069.1"
                     /translation="MVTSCTGHVLENQRATTRGDFSSGSHLVTLYFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(80792..81205)
                     /gene="pgaD"
                     /locus_tag="ISB17_000079"
     CDS             complement(80792..81205)
                     /gene="pgaD"
                     /locus_tag="ISB17_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAP1978070.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(81207..82532)
                     /gene="pgaC"
                     /locus_tag="ISB17_000080"
     CDS             complement(81207..82532)
                     /gene="pgaC"
                     /locus_tag="ISB17_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAP1978071.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(82525..84543)
                     /gene="pgaB"
                     /locus_tag="ISB17_000081"
     CDS             complement(82525..84543)
                     /gene="pgaB"
                     /locus_tag="ISB17_000081"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAP1978072.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(84552..86975)
                     /gene="pgaA"
                     /locus_tag="ISB17_000082"
     CDS             complement(84552..86975)
                     /gene="pgaA"
                     /locus_tag="ISB17_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HAP1978073.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
BASE COUNT        21421 a        22027 c        23005 g        20890 t
ORIGIN      
        1 agtgtaatat tccgtaacag gatgatcgtt atggcagggt tgcaaataga ttgcttgact
       61 taacaaacag ataacttgac agaaaggata aaaaaagagc cagcgggcgc tggctcttga
      121 agacactaaa ttagaagctg gcgttacgcg gagtacgtgg gaacggaata acatcacgta
      181 cgttttgcac gccagttacg taagcaatca ggcgttcaaa accaagaccg aaacctgaat
      241 gcggaacagt accgtagcga cgcagatcgc gataccacca gtaatcttct ttgttcaggc
      301 ccatttcaag catacgctcg tccagcacgt ccagacgttc ttcacgctgg gagccaccaa
      361 tgatctcacc gatgcccgga gccagaacgt ccatagccgc aacggtttta ccgtcttcgt
      421 taaggcgcat atagaacgct ttaatatctt tcggatagtt tttaaccact actggtgctt
      481 taaagtgttc ttccgccaga taacgctcat gctcagaaga gagatcgacg ccccagtaaa
      541 ccgggttttc aaacttcctg ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca
      601 cctgcgcgaa atcggcctca atgaagcgtt ccagacgtga aacggcatct ttatctacgc
      661 gttcagcgaa gaatttcatg tcgtcagcgc gttcttcgag aaccgctttg aagacatatt
      721 tcagcatggc ttcagccaga cctgcgatat cgttcaggtt agcaaacgcc acttccggct
      781 ccagcatcca gaattccgcc aggtgacggc tggtgttgga gttttcagca cggaaagtcg
      841 ggccgaaggt ataaattttg gacaatgcgc aagcgtaggt ttcgccgttc aactggccag
      901 atacggtcag gaaagactct ttaccaaaga agtctttgtc gaaatccact ttgccctgat
      961 cgttacgcgg caggttttcc agatccagcg tagaaacacg gaacatttcg cctgcacctt
     1021 cggtatcaga tgcggtaatc agtggcgttg aaacccagaa gaatccctgc tcgttaaaga
     1081 agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca atcaggtttg
     1141 tgcgcggacg caggtgagcg acttcacgca gatactcaat gctgtggcgt tttgccgcca
     1201 tcgggtaagt gtttggatct tcaacccaac cagcaacttc aaccttgctg gcctgaattt
     1261 caaattgttg cccctggccc ggcgacgcca cgactttacc cgttacaatg accgagcagc
     1321 cggtggtcag acgcaggacg tcttcattgt aattgggcag agaattattg atgacagcct
     1381 gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat
     1441 ctcggcgggt acgtacccat ccgcgcacgg tgacttcgct gtcaacggct acgcggccct
     1501 ggagtacgtc ggctacaggc acaacgctca taatattctc tctgttaata gtcggaaaaa
     1561 ataaacactt gtccacccgt aaatgggggg atacctatgt tacctggcat ctgcaatcag
     1621 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     1681 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     1741 gcagcgcccg aacaaacgct ttatcgtggc agatcgtttt gccagggctg tcagaaagtt
     1801 tcgccaccgg tttaccgtta cactctacca acttaatgac aatattcaga ggttttacct
     1861 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     1921 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     1981 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     2041 gcgggtcgcc agagtcatgg cgcaggccct ggtaacgact ggcaaattcc acgccgaaat
     2101 ctcgcaggaa agcatccatg gtgatgcagt cggttaacgc aatgccaagt tggtcgggat
     2161 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     2221 gctgatgcgc ctggaaccat tcgtgtgcct gtgttcccat tggcgtgagg gaaagccgac
     2281 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgtttcacga
     2341 tggtttcttg tacttcgcga gaaaaacggc ggcgggtgcc aaaatccatc agatggaagc
     2401 gcgacatatc aagaccggcg gttaatgccg agaagtcgac taatttgttt tccagcgtgt
     2461 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     2521 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cgggccgctt aaacgaatat
     2581 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     2641 gccagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     2701 cctgcaggcg caggtgctgc atcgcctgaa cctgttcacg aatagcatcg gcataaatac
     2761 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     2821 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     2881 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     2941 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     3001 tcacaccata tgccaccttt ctggtcgatg gcataacgcg gtgtatgtta taaaaatgta
     3061 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     3121 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     3181 cacaagccaa ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact
     3241 tgacctttga cctggacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     3301 atggtgcatc agatgttccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     3361 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
     3421 atttgccgga gcgttttacg cttaagatcg ttaatgaaat tagcccggcg gcgaatactg
     3481 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
     3541 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
     3601 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
     3661 gtgaactgga aaatggacgt cattgggtac agtggcagga tccattcccg aaaccgtgtt
     3721 acctgtttgc gctggtggct ggtgattttg atgtactgcg cgataccttc actacccgct
     3781 ctggtcgtga agtggcgctg gagttgtatg tcgatcgtgg caaccttgat cgtgcgccgt
     3841 gggcaatgac ctcgctgaaa aactccatga aatgggatga agaacgcttc ggcctggagt
     3901 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
     3961 ataaggggct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
     4021 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
     4081 gcaaccgtgt cacctgccgc gactggttcc agctcagtct gaaagaaggt ttaaccgtct
     4141 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc ggtaaaccgt atcaataatg
     4201 tgcgcaccat gcgcggattg cagtttgcag aagacgccag cccgatggcg cacccaattc
     4261 gcccggatat ggtcattgaa atgaacaact tctacaccct gaccgtttac gagaagggcg
     4321 cggaagtgat tcgtatgatc cacactctgc ttggcgaaga aaatttccag aaagggatgc
     4381 agctctattt cgagcgtcat gatggtagcg cagcgacctg tgacgatttt gtgcaggcga
     4441 tggaagatgc gtcgaatgtc gatctctccc atttccgccg ttggtacagc cagtccggca
     4501 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
     4561 tcagccagcg cacgccagct acgccggatc aggcagaaaa acaaccgctg catattccgt
     4621 ttgccatcga actgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
     4681 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
     4741 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
     4801 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctctcgct
     4861 gggacgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
     4921 aagggcagcc gttgtctctg ccggtgcatg tggctgacgc tttccgcgcg gtgctgctcg
     4981 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttcc gtcaatgaaa
     5041 tggcagaact gttcgatatc atcgatccga ttgctattgc cgaagtacgc gaagcactca
     5101 ctcgtactct ggcgactgaa ctggcagatg agctgctggc tatttacaac gcgaattacc
     5161 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
     5221 tgcgcttcct cgcttttggt gaaacgcatc tggctgatgt gctggtgagc aaacagttcc
     5281 acgaagcgaa caatatgacc gatgcgctgg cggcgctttc tgcggcggtt gctgcacaac
     5341 tgccatgccg tgacgcgctg atgcaggagt acgacgacaa gtggcatcag gatggtctgg
     5401 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
     5461 tacgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgc attcgttcat
     5521 tgattggcgc atttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
     5581 atcagttcct ggtggaaatg cttaccgacc tgaacagccg aaatccacaa gtggcttcgc
     5641 gcctgattga accgttgatt cgcctgaaac gttacgatgc caaacgtcag gagaaaatgc
     5701 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
     5761 tcaccaaagc actggcttga taaataaccg aatggcggca atagcgccgc cattcggaga
     5821 atttacccct gttttctcag gcgagtttca aattcaccac gctgcatcac ccgctgcaat
     5881 acttccgctt ccagctctgc taatcgtacc gaccctaacc ggcgcggacg ggggatatcc
     5941 accgtcaaat ccagaccaat ttttccctct tcaattaaca acacccggtc agccatcgcc
     6001 accgcttcgc tcacatcatg cgtcaccagc aagacggtaa agccgtgctc ctgccagagt
     6061 gatacaatca aatcctgcat ctcgagtcgc gttaaagcgt ccagcgcacc gagtggttca
     6121 tcaagcaaca ataaacctgg tcgatgaatc agcgcccttg ccagcgccac acgttgtttc
     6181 tgcccgccag aaagtgcggc aggccattcc cccgcgcgat tctccagtcc taccgcagcc
     6241 agagcccgac gcgcggcatc gcgccactgg cctttaaggc ctaacccaac gttatcaatc
     6301 accgatttcc acggcagcag acgcgcgtcc tgaaacatca ttcgcgtatc atcctgaatc
     6361 tcagccagcg gtgtggtgcc cgctaacacg tcgcctgcgg ttggcgtttc cagacctgcc
     6421 agcaggcgca gcagggtact tttgccacca ccgctgcggc ccaccaccgc cacaaactga
     6481 cctgctggaa tatgtaaatc cagttggttc aggacgatat tttccgcgta atgtttgctt
     6541 actgcattga gcaacaatgg tgtgccctgg ttcagacgag cagtattcat accgtggcct
     6601 ccttcaaatg ataagccggg ttccagcgca accagagacg ctctaacagc tgcgcgctga
     6661 cgtcagcaag tttgccgagc agggcgtaaa gaataatggc gactaccacc acgtccgttt
     6721 gcagaaactc ccgcgcattc atcgccagat aaccaatgcc ggaattggca gaaatagttt
     6781 cggcaacgat cagcgtcagc cacatcaggc ccaaagcaaa acgcacaccg accataattg
     6841 agggcagggc accaggcagg atcacatgga taaacagcgg catgccggat aatccatagc
     6901 tacgtgccat ctccaccagc tcacggtcga tattacggat gccatgccag gtgttgatat
     6961 aaatggggaa tagcgtaccg agcgccacca gaaagatttt tgcggattca tcaatgccaa
     7021 accacaaaat caccagcggg atcagcgcca gatgcggcac gttgcgcaac atctgaattg
     7081 aggtatccag cagtcgctca ccccagcgtg acagcccgct aatcagcccc agaattaatc
     7141 ccagcgatcc gccaattgaa aaaccaatca gtgcacgcca ggagctgatc gccagatgct
     7201 gccacagttc gccgctggca gagagcgtcc agaacgccat caccactcct tccggtgagg
     7261 gcaaaatacg cgtcgatagc cagccaaccg aggaggccag ttgccacacc gccacgatgc
     7321 ccaccggtaa aaaccagggg gcaacgcgca ataaccactt cttcactggc gttgccatca
     7381 tcgtgttcct tagctttgcg cgacgttacg ggggataaaa tcattcgcca ccgcttcgcc
     7441 ttgtggattc agcggttgtg gctggggaat ttccgggatg gcgacatcca gatgtgggaa
     7501 cagtaactcg ccgacccgat acgcttcttc cagatgcggg tagcccgaaa gcacaaaact
     7561 gtcgatgcca agtgcagcgt attcgttgat tcgcgcggcg accgtaggac catcgcccac
     7621 cagcgctgtc ccggcaccgc cgcgcactaa gccaacaccc gcccataaat tggggctgat
     7681 ctccagattg tcgcgcttgc cgttatgtag cgccgccatt cgctgttgtc ctacggaatc
     7741 cgttcgggcg aatgaggcct gtgctttggc gatagtttca tcatcaagat gcgagattaa
     7801 ccgctcggcg gcctgccacg cttcgtcgtt ggtttcacga acaatcacat gcagacgaat
     7861 accgaaacga attttgcgtc catgcgcggc agctttcgcc cgcacgtgtt cgattttctc
     7921 ttttaccagt ttcggtggtt cgccccaggt aaggtagaga tcaacttgtt ctgccgccag
     7981 ctcctgggcg acatctgacg atccgccaaa gtaaagcgga ggataaggct gttgaatcgg
     8041 cgggaagagc agtttggccc cgcgtacatg aatatgtttg ccgttgaaat ccacagtctc
     8101 gccaagcaac aaccgccgcc agacctgggt aaattctgcc gaggcttcgt aacgctcgct
     8161 atgatcaagg aacacgccgt cgcctgccag ttcttgggga tcgctgcctg tgaccaggtt
     8221 aaacaacgca cgtccgttag agagacggtc aagcgtggcg gcctggcggg cggcaacggt
     8281 aggtgaggtt acgctggggc gcagggcgac aagaaacttc agccgttgtg tcaccgggat
     8341 catcgacgcc gccaccagcc acgcatcttc acacgatcgc cccgtcggga tcagcacacc
     8401 ggtatagccc agacgatccg ccgcttgcgc aatttgttgc agataaccgt gatcaaccgg
     8461 acgtgaacct tcttccgttc ccagataatg cccgtcaccg tgggtcggta aaaaccagaa
     8521 catattcaga ctcataattg ttttccttcc agttgagtgg gctgccagat gcgctggcga
     8581 atatcgactt ttttcggcac cagacggttt tcataaaaca gatctgccgt ttgctgctgt
     8641 aaggcggcaa cttcagcgct caacggtttg atggtggtgg gaggacgatg atccagatac
     8701 gaggcaataa ccggtgccgg taagcccatc gtttttgcca gtaaagcgat gctttgttcg
     8761 cgctggctgc gggttaacgc atcggcctca ctaaaggttg tcagcacacc ctgaataaaa
     8821 gcgccgtttt tttctgcata cggacgagct gccagataaa acgatccagt ttgattgaga
     8881 tcggtgccgt ctttcagcac ccgcacgccg ccctgtaata atgcagcgga gtagtaggga
     8941 tcccagatag cccaggcgtc aacgttacct tgctggaacg cggcgcgggc atcggcaggc
     9001 gtcaggtaag tgggctgaat atccgtgaat ttaagcccgg cctggcgaag ggctcgcagt
     9061 aaaagattgt gcgaactgga acctttctga aaggcaactt tgtgaccttt aagatcggct
     9121 acggttttga tcgggctgtt ttctgccacc agaatcactt cggctttggg cttcggtggc
     9181 tcgacgccca cgtataccaa atcagccccg gcagcctggg caaagattgg cggaatatcc
     9241 ccggtactgc cgagatcgat actgccaacg tttaacgcct ccagcatctg tgggccagcg
     9301 gggaactcga cccaggagat ttttgtttgc ggatagcgtt tttccagtaa ctggtggctt
     9361 tttgccagca ccataccaat actgcctttt tgatagccta tacgtaacgc ttcaggcgag
     9421 gattctgcag caaccgcaat cgtagagacg ctaagtaaac ccgccagcac cagtttaatg
     9481 atcttacgca tgggcattac ctcgcaaaga cagtaggtca ggaacctgaa catcgcggcg
     9541 gtgcaacgcc tgccagaaag tttctagcgc ggtatcaaga cgagtttgca gatttggcgt
     9601 gaactggggt ttgtgttggt aatcaattac ttgcgagtca tcggcaaaca cgccgtgcag
     9661 gatctcctgg gctttcagcg tacttaaaac tggcttaagg gcataatcga ccgccagcag
     9721 atgggccacg gtgccgcctg tcgccagcgg cagcaccact ttgccttgca acgcacgttc
     9781 tggcagcagg tcgagcaggg ttttcagtgc accggaatag gcggctttat acacaggcgt
     9841 ggcgacaatc agcccatcgg cctgttgcag ctgttcggtg aaggtcttga gtgccggact
     9901 atcgaaacga gcataaagca gatcttccgg gacgaagttt tgcaaattcc agtgataaac
     9961 ctctacatcc aggccattta gtttttcccg cgcatattcc agcaaggagc tggagcgaga
    10021 aggaaatcga ggactacccg ccagggtgat gacacgcata ctctctcctt ataaccaatt
    10081 gttcttgttt tgttgacatt gataacaatt cggtcagtct gacagagggc gtccgcgagt
    10141 cgaaatgatt tatctgagga agaaaagcca aaaaaagcat aaaaaaagcg caataagaaa
    10201 acgtttgcta tttattgccg caggtcaatt cccttttggt ccgaactcgc acataatacg
    10261 cccccggttt gcacaccggg aatccaggag agttcatgta ctaccccttc gttcgtaaag
    10321 cccttttcca gctcgatcca gagcgcgctc atgagtttac ttttcagcaa ttacgccgta
    10381 ttacaggaac gccgtttgaa gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact
    10441 gcatgggcct gacgtttaaa aatccgcttg gtctggcagc cggtcttgat aaagacgggg
    10501 agtgcattga cgcgttaggc gcgatgggat ttggatccat cgagatcggt accgtcacgc
    10561 cacgtcccca gccaggtaat gacaagccgc gtctctttcg tctggtagat gccgaaggtt
    10621 tgatcaaccg tatgggcttt aataatcttg gcgttgataa cctcgtagag aacgtaaaaa
    10681 aagcccatta tgacggcgtt ctgggtatta acatcggcaa aaataaagat acgccggtgg
    10741 agcagggtaa agatgactat ctgatttgta tggaaaaaat ctatgcctat gcgggatata
    10801 tcgccatcaa tatttcatcg ccaaataccc caggattacg cacactgcaa tatggtgaag
    10861 cgctggatga tctcttaact gcgattaaaa ataaacaaaa tgatttgcaa gtgatgcacc
    10921 ataaatatgt gccgatcgca gtgaagatcg cgccggatct ttctgaagaa gaattgatcc
    10981 aggttgccga tagtttagtt cgccataata ttgatggcgt tattgcaacc aataccacac
    11041 tcgatcgttc tctggttcag ggaatgaaaa attgtgatca aaccggtggc ttaagtggtc
    11101 gtccgcttca gttaaaaagc accgaaatta ttcgccgctt gtcacaggaa ttaaacggac
    11161 gcttgccgat tattggtgtt ggcggcattg actcggttat cgctgcgcgt gaaaagattg
    11221 ctgcgggtgc ctcactggtg caaatttatt ctggttttat ttttaaaggt ccgccgctga
    11281 ttaaagaaat cgttacccat atctaattat ttcttcaaat tcctctttat aagataatca
    11341 gggctttatt ttcagccctg gttgttttat attcatcgct gttgcttatt tagacatttt
    11401 gtacttttat tgttgaggtt attaagcgaa gcgacaatgg attgtgtcgc tgcggtttta
    11461 taaggttggg gagtggcaga tgcgaattaa accagacgat aactggcgtt ggtattacga
    11521 tgaagagcac gatcgaatga tgctcgattt agccaatggt atgctatttc gctcacgttt
    11581 tgcgcgcaaa atgttgactc cagatgcctt ttctcccgca ggtttttgcg ttgatgacgc
    11641 cgcgctctat ttctcttttg aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa
    11701 agccgaactg gtgctgaatg cactggtagc aattcgttat ctcaaaccgc aaatgccgaa
    11761 aagctggcat tttgtatctc atggtgaaat gtggatgccc atgccgggcg atgcggccta
    11821 cgtttggttg agcgacactc acgagcaagt gaatttactg gtcgttgaat ctggcgagaa
    11881 tgccgcgcta tgtctactgg cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt
    11941 gggcgacgcc attaaaatca tgaacgacag gctgaaaccg caggttaatg ttgacagctt
    12001 cagcctcgaa caggcagtct aacgcgccag cttaagcgca gtcttcggta cacaactaca
    12061 gcaaagaata gtgccatcat cgcctgttgc tgattttttt agcggcgtga cttcgccttc
    12121 taaaagctga acacggcaac ttccacaaat gcctgcgcgg caagaataag ggatacgaat
    12181 tccctgattt tctaattgct ccagcagcac ctgttggtta tttccacgaa atgcctgtcc
    12241 ctgccagtca atatctacgt ttgcgtccgg ttgttgcgtg atgttggcag taccatcagc
    12301 ggcacctgcg ccgtaaattt tagctggagc cgttgccaga atttccacct catcgccaac
    12361 gcgaatcacg ccgctattac gggcaattaa attctgacca aaatcgacat cgccgttatc
    12421 ctgggcagta cggaaagatt gcagcgtttt taatggttcg tctgccggat gtttttgccc
    12481 tttttctggg ctgacggtgg tgaaaataca gcggctacaa ggcttaacca catcaaacac
    12541 gacatcacca atgcgaatca ctttccagct atcttcttcc caggccgatg caccggaaac
    12601 caccagattg gggcggaatt gctccatttt tacactggcc ggacaacgtt gttggagagc
    12661 acgtaacgag gcctcgttag caagaaggta aggatagcca tcagcaaatg acaggggtac
    12721 agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc acccagcgta attgcacttc
    12781 gcgggagaaa aatccactta gccatttgtt gatcgcatct ggcgcaattc gcgcggtaaa
    12841 atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata
    12901 tgcgctactg ccatctggtg cggtgagatg caagccatca tgcacgggtg aaggagtaaa
    12961 ccgtaccatt agcggaaact ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat
    13021 aaagatgcga tcgaaggcca gaccactgac atctgccagg gtatgtgtaa gaccaatgcc
    13081 gcgcatcgat ttaacaggat gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc
    13141 aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt attagctata atgcgcaaca
    13201 attttcttag agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct
    13261 ggaagagctg ttaaaaactg aactggaaaa cctgggggcc gttgaatgcc aggtggttca
    13321 gggtggggtc catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg
    13381 cctggcctcg cgtattatgt tgccgctggg cgagtgtaag gtttacagtg atttagacct
    13441 ctatctcggc gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc
    13501 tgtccatttc agtggtttaa atgacaccat tcgcaacagc cagtacggcg cgatgaaagt
    13561 gaaagacgcg atcgtcgatg ctttcacgcg gaaaaatctg ccacgtccca atgttgatcg
    13621 cgatgcgccg gatattcgcg ttaacgtctg gctgcataaa gaaaccgcca atatcgccct
    13681 tgatctcagt ggtgatggct tgcatctgcg tggctatcgt gatcgtgctg gtattgcacc
    13741 gatcaaagaa accctggcag ccgcgattgt gatgcgatcc ggctggcagc caggaacacc
    13801 actgctcgat ccaatgtgtg gttctggtac gttgctgatt gaagcagcga tgctggcgac
    13861 cgaccgcgca ccgggcttgc accgtggccg ttggggcttt agcggctggg cgcagcatga
    13921 tgaagctatc tggcaggaag tgaaagcgga agcgcaaact cgcgcccgta aaggcctggc
    13981 agagtatagc tctcattttt acggttcgga cagcgacgcc cgggtgattc aacgtgcgcg
    14041 cactaacgcc cgtcttgcgg ggattggtga actgatcacc tttgaggtga aagatgtcgc
    14101 gcaactgacc aatccgctgc cgaaagggcc gtacggtaca gtgttgagca acccgccata
    14161 cggtgaacgt ctggacagcg aaccggcgct gattgcgctg catagtctgc tgggccgaat
    14221 catgaaaaac cagttcggtg gctggaatct ctctttgttt agtgcctcgc cggatctgct
    14281 aagctgcctg cagctgcgtg cagacaaaca gtacaaggcg aaaaacggcc cgctggactg
    14341 cgtacagaaa aactaccacg ttgccgaaag cacaccagac agcaaaccgg caatggtagc
    14401 ggaagactac gccaaccgcc tgcgtaagaa cctcaaaaaa ttcgagaagt gggcacgtca
    14461 ggaagggatt gaatgttatc gcctgtatga tgccgatctg ccggaatata acgttgccgt
    14521 tgaccgttat gccgactggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc
    14581 gcacaaagcg cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tactggggat
    14641 tgcgccaaac aaactggtgc tgaaaacccg tgaacgccag aagggcaaaa atcagtacca
    14701 gaaactgggc gagaagggcg agtttcttga agttaccgaa tataacgctc acttgtgggt
    14761 gaacttgacg gattacctcg ataccggtct gttcctcgat caccgtatcg cccgtcgtat
    14821 gctcggtcag atgagcaaag gcaaagattt cctcaacctg ttctcctata ccggtagcgc
    14881 caccgtgcat gcgggattag gcggcgcgcg cagcaccacc accgtggata tgtcgcgtac
    14941 ttatctggag tgggcagaac gcaacctgcg tctgaatggc ctaaccgggc gtgcgcatcg
    15001 cctgattcag gccgattgcc tggcgtggct gcgtgaggca aatgaacagt tcgatctgat
    15061 ctttatcgat ccgccaacct tctctaactc aaaacgaatg gaagatgcgt ttgatgttca
    15121 gcgcgatcat ctggcgctga tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat
    15181 catgttctcg aacaacaaac gcggcttccg tatggatctc gacggcctgg cgaaactggg
    15241 actgaaagca caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca
    15301 gatccacaac tgctggctga ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt
    15361 atgcatggcg catggctgtc gttcagcgac gcgccgcttc tcgataacgc agaactgcat
    15421 atcgaagata acgaacgtgt ttgtctggtg ggtcgtaacg gcgcaggcaa atcgacgtta
    15481 atgaagatcc tcaaccgtga acaggggctg gatgacggtc gcattattta cgagcaagat
    15541 ttgattgtag ctcgtctgca acaggatccg ccgcgtaacg ttgagggtag cgtttatgat
    15601 ttcgttgccg aaggcattga agaacaagcg gaatacctga aacgctatca cgatatttcg
    15661 cgcctggtga tgaacgaccc gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa
    15721 cagctggatc accacaacct gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa
    15781 ctgggattag atcctaatgt tgcgctgtcg tcgctttccg gtggctggtt gcgtaaagcg
    15841 gcattaggac gcgcgctggt gagcaatccg cgcgtattgc tgctcgacga accgactaac
    15901 cacctggata ttgaaaccat cgactggctg gaagggttcc tcaaaacctt caatgggaca
    15961 attattttca tttcccacga ccgttcgttt atccgcaata tggcgacgcg cattgttgat
    16021 ctcgatcgcg gcaagctggt gacctatcca gggaattacg accagtacct gctggaaaaa
    16081 gaagaagccc tgcgcgtgga agaattacaa aatgccgagt tcgatcgcaa actggcgcag
    16141 gaagaggtgt ggatccgcca ggggatcaaa gcacgtcgta cccgtaatga aggccgcgta
    16201 cgcgccctga aagcaatgcg tcgtgagcgt ggtgaacgtc gcgaagtgat gggtaccgca
    16261 aagatgcagg tggaagaagc cagccgctcc ggtaagatcg ttttcgaaat ggaggacgtt
    16321 tgctaccagg ttgacggtaa gcaactggtg aaagattttt ctgcccaggt tctacgcggc
    16381 gacaaaattg ccctgattgg tccgaatggg tgcggcaaaa ccacgctgct gaaactaatg
    16441 ctcggtcagc ttcaagcaga cagcgggcgt attcacgttg gcaccaaact ggaagtggct
    16501 tatttcgatc agcaccgcgc ggaactggat cccgataaaa cggtgatgga taaccttgcc
    16561 gaaggtaagc aagaggtgat ggttaacggc aagccacgcc acgtattggg ctatttgcag
    16621 gacttcctgt tccatccaaa acgggcgatg acgccggtac gtgcgctttc tggcggtgag
    16681 cgtaaccgct tgttgttggc gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac
    16741 gaaccgacca acgatcttga tgtcgaaacg ctggaactgc tggaagaact gatcgacagc
    16801 tatcagggca cggtattgct ggttagccac gatcgtcagt ttgtcgataa caccgttaca
    16861 gaatgctgga tcttcgaagg cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat
    16921 gcccgtggtc agcaagaaca gtatgtggcg ctcaaacagc ctgcggtgaa aaaaagcgaa
    16981 gaagccgccg cgccaaaagc agaaactgta aaacgcagca gtagcaaact aagctataaa
    17041 ttgcagcgtg aactggagct gctaccgcaa ttgcttgaag atctggaggc gaagctggaa
    17101 gctttacaga cgcaagtggc ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa
    17161 aaagtactcg ctgatatggc tgctgcagag caggagctgg agcaagcctt tgaacgctgg
    17221 gagtatcttg aagcgttaaa aaatggtggc tgatcgcaaa atagtcgatt taaacagaga
    17281 ctgctgacaa cggtaaaatt gcctgatgta ctgggtttat caggcatgaa aaccaacact
    17341 ttgttagtca tctgcaaaat agcgcatcat taaggagtac caatgtgcga acatcatcat
    17401 gccgcgaagc acatcctgtg ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag
    17461 catggtcaga aagcggcatg tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc
    17521 cctcggcagc gtcccaccgc ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac
    17581 ttgtttcctt ttgtgaatat gaacgttgca ggagttacca gtgaaattac attactggaa
    17641 attcccggcg tgcttttttc tgaggactac gccagcctcg gcaccttttt cctgttgttt
    17701 gtgcaactgg ttcccgcgtt ttgtctgata accattctgt tactggtgaa tcgcgcggaa
    17761 ttaccggtcc gtttaaaaga gcaactggca cgggtgcttt ttcaactcaa aacctgggga
    17821 atggcggaga ttttcctcgc gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc
    17881 agcattgggg taggcagcag ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc
    17941 gcttttcagt gcgttgatcg tcgctggtta tgggacgaca tcgccccgat gccagaactg
    18001 cgccagccgc taaaaccagg cgtcacgggg atacgccagg ggctgcgttc gtgttcctgt
    18061 tgtacggcaa tccttcctgc tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac
    18121 gttcggcgta gaaacagcct gcaatggaca ctcgcgctgc ttgtaacgtc tatcatgctg
    18181 tatcttccgg ctaatatttt gcccatcatg gtgacggatt tattaggctc gaagatgccg
    18241 tcgacgattc tcgctggggt cattctgtta tggagcgaag gctcttatcc cgtcgctgcg
    18301 gtgatttttc tggccagtat tatggtgcca acgttaaaga tgatcgccat tgcgtggctg
    18361 tgttgggatg ccaaaggaca cggcaagcgc gacagtgaaa gaatgcattt gatttatgaa
    18421 gttgttgagt ttgtaggccg ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg
    18481 gcgctggtgc gtatgggagg tttaatgagt atttatccgg caatgggtgc attaatgttt
    18541 gctttagtcg tcataatgac aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg
    18601 gatcgtcaac ctgaatcaga gcatgaggag tcctgacagt atggaatcta ataatgggga
    18661 agccaaaatc cagaaagtga agaactggtc tcccgtgtgg atatttccta tcgtcacggc
    18721 gctcattggg gcctgggttc ttttttatca ttacagccat cagggaccgg aagtgaccct
    18781 gatcaccgcg aatgcggaag gaattgaagg tggcaaaacc accattaaaa gccgtagcgt
    18841 tgacgtcggc gtggttgaaa gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa
    18901 agcgcggctg aattccggta tggaaaaatt gctgcataaa gataccgtct tttgggtggt
    18961 gaaaccgcag attggtcgag aagggattag cggcctggga acgctgctgt ctggggttta
    19021 tatcgaactg cagccaggcg cgaaaggcag caaaatggat aaatacgatt tgctggactc
    19081 gccaccgttg gccccgcctg atgcgaaagg tatccgtgtg cttctcgata gcaaaaaagc
    19141 cgggcagctc tcgccagggg atccggtgct gttccgtggc tatcgggtag gttcggttga
    19201 aaccagcact ttcgatacgc aaaaacgcaa tatcagctat caactgttca tcaatgcacc
    19261 ttatgaccga ctggtgacca gcaatgttcg tttctggaaa gatagtggca ttgcggttga
    19321 tctgacgtca gcgggaatgc gtgtggagat gggctcattg acaacgctgc ttagtggcgg
    19381 tgtcagcttt gatgtgccgg aaggtctgga tttagggcag ccagtggcac cgaaaacagc
    19441 tttcgttttg tatgatgatc agaagagcat tcaggattcg ttgtacaccg atcacattga
    19501 ttatctgatg ttctttaaag attcggtacg cggtctgcaa ccgggagctc cggtagaatt
    19561 ccggggtatt cgcctgggta ccgtaagcaa agtgccattc tttgcgccga atatgcgtca
    19621 gacatttaac gatgattacc gtattccggt actgattcgt atcgagccag agcggctgaa
    19681 aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg
    19741 tttacgcgga tcgctgaaaa ccggaaacct ggtcactggc gcactgtatg ttgatctcga
    19801 tttctatcca aatacgcctg caataaccgg tattcgtgaa tttaatggtt atcagattat
    19861 cccgaccgtc agcggcggcc tggcgcaaat ccagcaacga ctgatggaag cgttggataa
    19921 gatcaacaaa ctgccactga atccgatgat tgaacaggca accagtacgc tttctgaaag
    19981 tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc atgaacaaga tcctcgccag
    20041 ccagtcgatg cagcagttac cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag
    20101 tatgcagggc ttccagcctg gttccgcagc ctacaacaag atggtggcgg atatgcagcg
    20161 ccttgatcag gtgttgcgag aactgcaacc ggtgctgaaa acgctcaacg agaagagtaa
    20221 cgcgctggta tttgaagcga aggacaaaaa agatccagag ccgaagaggg cgaaacaatg
    20281 aaaaagtggc tagtgacgat tgcggcactg tggctggccg gatgcagctc cggtgaaatt
    20341 aataaaaact attaccagtt acctgtggtg cagagcggta cacaaagcac cgccagccag
    20401 ggtaatcgcc tgttatgggt tgagcaggtc gctgttcctg actatctggc ggggaatggt
    20461 gtggtttatc aaaccagtga tgtgaagtat gtgattgcca acaacaactt gtgggccagc
    20521 ccgttggatc aacagttgcg caacaccctg gttgccaacc tgagtacgca actgcccggc
    20581 tgggtggttg cctcccagcc tctgggaagc gcccaggaca cgctcaatgt taccgtcacg
    20641 gagtttaacg gtcgctatga tggcaaggtc attgtcagtg gtgagtggct gttgaaccac
    20701 cagggacaac tgatcaaacg tccgttccgt ctggaaggag tgcaaactca ggatggttac
    20761 gatgagatgg ttaaagtgct ggcccgtgtc tggagtcagg aagccgcttc tattgcgcaa
    20821 gagataaagc gtctacctta atcataaaga tttgtaaata taaccgtctc cggtatgttg
    20881 cctgaggcgg tttttttgtt tctaacgtgc ggaaaaagtt gttcctcttc acattttttg
    20941 tacaaccgac atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga
    21001 cggcagttat gattcgcggt attgcttaac tgtggttgca tatttagtaa tcactgtttt
    21061 cttttccacc agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc
    21121 ctggaacggg cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg
    21181 tgtccctatc agacgctgaa tcaaaggtca caatggctgg gaggctggcg agaagccatg
    21241 gcggacaggg tagtaatggc ctgattctgt ctctttaaaa agaaacctcc gcattgcgga
    21301 ggtttcgcct ttttatactc tgtctgatta ttatcagaag gcagacgtat cctggaacag
    21361 accgactttc aggtcgttgg cggtatagat caaacgacca tcaaccagca cttcgccatc
    21421 agccaggccc ataatcagac gacggttaac tatgcgttta aagtgaatac gataggtcac
    21481 ttttttcgcc gtcggcagca cctgacctgt gaatttcact tcacctacgc ccaacgcgcg
    21541 acctttacct tcgccgccca gccagccgag gtagaaccct accagctgcc acattgcgtc
    21601 caggcccagg catcccggca taaccggatc accaatgaag tggcatccga agaaccacag
    21661 atccggattg atatccagct ctgcttcaac ataccctttg tcgaagttac cacccgtttc
    21721 ggtcattttg accacacggt ccatcatcag catgtttggt gctggcaatt gcgggccttt
    21781 agcgccaaac agttcaccgc gaccagaggc aagaaggtct tcttttgtat aggattcgcg
    21841 tttatctacc atgttctctg taagccttat tttattgaag cacgcaggat agctaacacg
    21901 tgtacgctga acaagtccga tcagttcgga ataaaccagt tcagccaacg taatggccat
    21961 ggaaaacggt gacgtccttc ctgttgcgat gcttgtgcga tacgttcctg gatggtttgc
    22021 atcagcgtcg tctggccttc gccatcccac accagattta ataataacgg cagtgcgtca
    22081 gtcacatcgt ctactgccca gatggtgaat ttgccttctt ctaccgcttt caccagttca
    22141 ctgtgaagac ttaaatggcg aacgttagca gtggggataa tgacaccttg tttccccgtt
    22201 aactcacgtt gctggcaaat agcaaagaag ccttcgattt tctcatttaa accaccgact
    22261 ggctgggcgc gaccgaactg atcgactgaa cctgtgatag cgatactctg attcaccggc
    22321 acatcggcga gggcgctgat cagagcgcag agttcagcca tcgaggcact atcgccatcc
    22381 acttcactgt atgactgctc aaatgtcagc gatgctgaga aggggatctg ttgctcaagc
    22441 tgtagttccg acatcaggaa cgcttgcatg atcatcatcc ctttcgcatg gatattgccg
    22501 ccaagctccg ctttgcgttc gatgtcggtg aattcaccat cgccaatatg cacaacgcag
    22561 ctaatgcgag aaggttcgcc aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg
    22621 gcgttaattt gcccgatgcg ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc
    22681 tcatcctgca tgcgttcagc gaggaaacct tcgcgccatt cacgctgctg tagcattaag
    22741 tttagctgct cgccggagaa ggtgtcgcca tcacacaggg aggcgacctc ttgacactgg
    22801 cggaggatcc actgcgggct aagcggaagt gtttcttgtt caccagtgta gcgtgctgct
    22861 tcgcggataa gtaccggcca ggcatccgct cccggtgctg gtaagtgatt atgtctggcg
    22921 gtaaatgtca cccagcgaca ccactggctt actgactccg catcgacaat ctgcagagta
    22981 tcttcaaatt cgctataaat agcctgctct gaaagctctg gctccatctc ctggaaatca
    23041 gccaatgatt cgcgttcgcc taccagaatg actttcagct tcaatggcat cgaaggcaca
    23101 gagacgggga gagggcgcga ctcatcaaac gcaacccagt caaaacgctc gcggttaacg
    23161 atatttttca gccgcatcca cagcagaggt tgcgccagca gtgtacgcaa agagatgatg
    23221 agaataccgc catttgcctg atgcaccaga ccaggctgca gggtaatatc gccattaaac
    23281 tgacgcaggc agccaaagag ttgctctgct tctacccagt cggcagcgac aacttgcgtt
    23341 aaagtcgcaa aattatcatc tgcactcact gcgtgacgta agcggatggt gtggccggaa
    23401 acctcatagt gaccgcccac cagttggcct gcatcgcttt gtagcgtacg cgcggcatca
    23461 gcaataagat tcagatactc agactcttcc ggggccttcg ccagcataaa agaggaggat
    23521 gctcgcgtat gcagcaactg ctccagcgca aattgcagtc gcggttgagt atcactgaat
    23581 aaaggatcgt tttcgtcaat caaatgtggc tgagcaaata tttcctgata gctatcggta
    23641 tcaggaacca ggtcacgcca tgcaagtttc gtaatggtca aagttgatgt tttttagtct
    23701 gttgtcaaag ccgcgattat accgtaaccg gcactgcagc acacgtagaa agcaacgaca
    23761 atacgcctgg catgggcgtt aaagctcaca ggatggagat tcttttcttc actggcctaa
    23821 aaagctgata ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca
    23881 acttgaaaat cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc accgcgaagg
    23941 ggagttaatc acccgttaca tagaagccag tgccgcccag gaagccgtcg atgagttgct
    24001 ctcgctggaa aatgaacccg tgctggtaaa tggttggatt gacaagcata tgaatccgga
    24061 actggtcaat cgcatgaagc agaccattcg ggccagacgg aagcgccatt ttaatgcgga
    24121 acaccagcat acgcgcaaaa aatcgatcga tctggaattt atcgtctggc aacgtctggc
    24181 tggccttgcg cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga
    24241 tgcggaaaac aaagagaaat acgcgaataa aatgtcttct ttgaagcagg atctgcaggc
    24301 attgctgggt aaggaataac tgtcaaaagt cagttcaatt tacgcaaggc gaaaaaaaac
    24361 cccgcagcag cggggttttt taccagacga gaacttaagc ctgcggctga gttacaacgt
    24421 ctttgatgcc tttaacttcg atctctacgc gacgatccgg agccaggcaa tcgatcagtg
    24481 cagcacgctg tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac
    24541 cacgtgcgga gattttgtct gccgggatac ctttagagat caggtaatca acaacggact
    24601 gagcacgacg ctcggacaga gcctggttat aagcgtcaga accgatgcgg tcagtgtaac
    24661 ccagaacaac tacggaaccg tctttcggat ccaggttgct cagctggctg tacagctgat
    24721 ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt gaaggtgaac agaacgtcag
    24781 acttcagagt gaagtgcttg gtctgtactt ccggtgccgg agctggagcc ggagcaacta
    24841 ctggagctgc ttcgccctga ccgaaacggt aggaaacacc caggctcagc atgccgttgt
    24901 ccggacgagt gccgatggtg tgtgcatcac cgatgttgtt ggtccactgg tattccagac
    24961 gggtagcgat ttcgggagtg atcgcatact caacaccgcc agcgaagacc ggagaaacgc
    25021 cggtgtcgtg gtctttaaag gatgcgccac caggtacgtt agccttggtg tctgcacgcc
    25081 ataccatacc acccagacga gtgtagatgt ccagatcgtc agtgattggg taacccagtt
    25141 tagcggtcag ctgaacgccc tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg
    25201 gcatacgacc taaccagtcg taacccattt caaagccaac atacgggtta acctggtaac
    25261 caccaaaagc acctgcaccc agttggtttt cgtgggtcgg accattgtta ggaataaaac
    25321 cagtgtcatg gtactgggac cagcccagtt tagcaccagt gtaccaggtg ttatctttcg
    25381 gagcggcctg cgctacggta gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct
    25441 ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca tgaatatctc caacgagata
    25501 acacggttaa atccttcacc ggggatctgc tcaatattaa ctctaccgat atcttcggct
    25561 tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga
    25621 actccgtcag gcatatgaaa aaaaagtctt gtataaggta cgtttaatct tttttgtcag
    25681 cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt
    25741 agtcacgact gaaagcattg gctgggcgat aaaaaaagtt ccaggattaa tcctaaattt
    25801 acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt tgcgtgagag
    25861 gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca
    25921 agttcagcat gctcttctgc agtcaaatca tctgccaacc aaccgatcac cacgctgtaa
    25981 ttgcccgtgc gtaaagcgcg aaccattgac tccacggtgt ggcaagggga gagctggcta
    26041 atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcgcg actcagtttt
    26101 tgttgcggtg ttaaccagag ttgccagcgt gattgctgac cgagttgctg taacaatggc
    26161 aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc
    26221 ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac
    26281 gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt
    26341 atgaatgtac agtacatcca gtgacgacaa agatcaaccc tattttcgga aagagcctcg
    26401 caaattttgt cgttggtggc gggaaaacat aaattaatct tgccccttaa gaataagttg
    26461 cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa
    26521 aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc
    26581 aacgttgggc acaattgaat accgatcatt gtttggtagt tacagcctga ccgttgacga
    26641 cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc
    26701 acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt
    26761 taccctcaat tactatcgag ttgatgaaag tctatggcga aatcaactga agctggtgcg
    26821 tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga
    26881 aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg
    26941 gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg
    27001 gcagcaaaac tgtctggtga cagaaaagat tctgtttatg cttgaagggg ccattatcgg
    27061 cattcatgaa gctgcgctcc cagtggcacg ccgccaggag cttgcagaat gggctgactc
    27121 tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca
    27181 atctctggca gtaatgcaat caataaccca acttgttgta cgaccagagg ttctttgctg
    27241 tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc cagctaatgc ctgattgacg
    27301 cgttcttcat cagcaggttg atgatgtagc gcgtcatcaa catagcaaac ggcgtcatca
    27361 agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata
    27421 tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg
    27481 gtaacgttcg gttcgctgga catatttgat accaccgacg ccagttcagc atcacggttg
    27541 tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgt
    27601 tccagtatgg catcgagata ccggcagttg gcctctgtgg cgcgttcgag cattcgcggc
    27661 agattgcgaa acttccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa
    27721 ccaatcagcg tatcgattac gcgaggtaac gccacttcaa aaccttcacc cagcaagttg
    27781 aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gggcgtattg cacgttacgg
    27841 aaggcaaaaa agagcacgcc ggtaataacc agcagcacca gctgcccttc caatgatggc
    27901 acaaaccaca gcacaggaat accaattgcg atacctacca gcgtaccaat aatccttaac
    27961 ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag
    28021 atccaatacc cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa gcacaacacc
    28081 agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga
    28141 cgcagccaga tatcactcaa tccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta
    28201 ttatggggca gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggca
    28261 cgtaaattgt tcagtaaaaa tcccagtgtt ttgagtaaat cagcaggcgc gccgttatcg
    28321 cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt
    28381 tgataaggct gacgcagcaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc
    28441 gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt
    28501 tgatattgaa tatgagaaga gctggcacgc tcgtgaatat cctgcgcgac aaagtaataa
    28561 tgcagtgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag
    28621 agtttcgtct gattcaatgt cgccatcagc tgaccgttgg cgagagccaa atcgtacagc
    28681 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
    28741 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
    28801 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagaag atacatcggc
    28861 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
    28921 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
    28981 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
    29041 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
    29101 cggtcatcga gatcggtcag cgccgctgcc accatcccaa gcgtcagcgg aatcgtcagt
    29161 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
    29221 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
    29281 gccatattag tggattattc aaaacgacga cgtgcattgg cttcgcgcgc agcttgcgct
    29341 gtttctaccg ataccacgcg acgaccgacc ggccatagtg caatagcggc aattttaaag
    29401 ttcgcaatac cgacaggaat gccaatgatg gaaatacatt gtgcgatgcc cgttgcaatg
    29461 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
    29521 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
    29581 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagtggtaag
    29641 gtaaaaatca gcacaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
    29701 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
    29761 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
    29821 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
    29881 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
    29941 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
    30001 tcactgtaag cgcggtctgg tatggggcga actggagttt gtactgccgg acgaaaaagt
    30061 ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
    30121 tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggcgttt tacgtcagca
    30181 actggatttg attgccacgc gcaccggaga aaataaatgg ctgacgcgtg agcaaacctc
    30241 tggtatgcag caacaaatcc gccaggcttt gtcggcgtta ccattgccgg ttaaccgact
    30301 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagacat
    30361 tgaaggcgct cggttgcagc ataatcaggc gtataccgaa gccatgctta ccgagtatgc
    30421 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt
    30481 taatggcgag cattctttgt tagtgctggc tggtgcagga agcggaaaaa cgtcggtgct
    30541 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
    30601 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgag aacgactaca
    30661 taccgaagac attaccgcac gcacatttca tgcgctggcg ctgcatatta ttcagcaggg
    30721 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact
    30781 ttttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
    30841 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaatttct gggatgatga
    30901 aaaattacag cgtcgtcttg cctctcgcct cgatcgctgg gtaagtctga tgcggatgca
    30961 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
    31021 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
    31081 aaacgctgtc gatttttcgg gccttattca ccaggcgatt gtgattctgg agaaaggtcg
    31141 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
    31201 tgcagcgttg ttagcggcat tacgcaagca aaatagtcaa acgacgttgt tcgctgttgg
    31261 tgatgactgg caggcgattt accgattcag cggggcgcaa atgtcgctca ccacggcttt
    31321 ccatgaaaac tttggtgaag gtgatcgctg tgatttagac acgacctacc gttttaacag
    31381 tcgtatcggt gaggtggcaa acaggtttat tcagcagaac ccaggccagc ttaaaaagcc
    31441 gctaaacagc ttaaccaatg gagacaaaaa agccgtcaca ttattggatg aggggcaact
    31501 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttctgatcct
    31561 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
    31621 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat
    31681 catcgttggc ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat
    31741 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
    31801 gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc
    31861 ctctcctttt gtcgaaatac tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga
    31921 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
    31981 taatcgggga tcagaatatc gaccgcgtcg ttaaaatgcg gtgactggat aatgaagtct
    32041 gccgttgcca cgttggtggc gaccggaatg ttccataccg ttgccagacg cagcaaggct
    32101 ttcacgtcag gatcgtgcgg cacggcattt agtgggtccc agaagaaaat caatacatca
    32161 attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac
    32221 atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt gcctgttgca
    32281 tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt
    32341 ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg aaggtaaagt gcgagtcgtc
    32401 agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagt acttatcgct
    32461 gcaagataaa gactgaaaaa gcctgcgcac aggcacaaaa atctcaggag gatggttgtt
    32521 tttccgccca ttgcaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact
    32581 tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac
    32641 ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt
    32701 tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg
    32761 taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa
    32821 ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa
    32881 aattgacctg gttgatgagc tgggtattaa aactaaccac cagtggaggg gagatataga
    32941 gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg
    33001 gtccattgtc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttac
    33061 cgtcgagtac cagaagatcg acatcagtag aaagccgcag cgtggtggca aaaacggata
    33121 ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcatagat agctcctgtt
    33181 aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat
    33241 agacatattt cccgcttttg ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg
    33301 ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag
    33361 gcattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc
    33421 ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt cctgtttcgc
    33481 caaaagttgc cggcaaaaca ctgctgggcc agcaggggta tggcacgctg gcagacgttc
    33541 cggaaaaagt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcgc
    33601 aggaagctat cgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc
    33661 aagcggcagt actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta
    33721 ttgagatccc tcgcctgggc ctggcaaaat aaaaaatacc cggaaggcga aaaccttccg
    33781 ggtatgttta tgagaattag ttacgcagac gcggcgcctg gagttgtttg cggatggtct
    33841 gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct
    33901 gggcttcagc caggtaggta tgaaccggta ggccgttatc atcctccatc accacgtgat
    33961 accacggagc ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac ggttcagaga
    34021 gcgagtaaac cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga
    34081 cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct
    34141 ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca
    34201 gatatagccc ttccgggtag gtagcgatca ccggatgatc ggcggcctga cggaactgct
    34261 ctataaattg tacatcacga ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata
    34321 aatcgctggt catcagaccg gaacaggaga aagtcaggag aacaccgcct tcattcagca
    34381 actgaatcgc cagcatgttg atgtctttat agccacggca cgcgcccatc aactggcttt
    34441 tattctcaac aaacttcggc gggtccatca cgataacatc aaatttttca ccgcgatcgc
    34501 gataagtacg cagcaattta aagacatcat cacggacaaa ctctgccttg ctcagatcca
    34561 gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc
    34621 tgacaacctg gctgcaaccg cccatcaatg ccgatacggc gaaaccaccg gtataggaga
    34681 aacagttaag tacgcgttta ttttcgacgt agcggcgggt agccaggcgg ctgtcacgct
    34741 ggtccaggta gtagcccgtt ttgtgcccgt gctgaatatc caccagcagt ttcatgccgt
    34801 gttcttcaat cggcagcagg gcaggcggca actcgccggt gatggggccc tgagtcagct
    34861 ccatcccttc ttttttacgt accgcgacgt cgctgcgatc gtaaatggca cattccgggt
    34921 acaacgtttg cagggcgcta attaatgccg cgcgctgata ctctgcgcca gcactcagca
    34981 attgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt
    35041 caccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt
    35101 tttgtgcttg ctgcaaacgg cgggtaaaaa aagcaatgtc gatagactca gacgggtcaa
    35161 acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt
    35221 ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg
    35281 caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg
    35341 ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat
    35401 tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt
    35461 cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga ttccgctaca
    35521 ccacgcagta cgaagcgaaa aaactgggac taaccgggta cgccaaaaat cttgacgatg
    35581 gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta atgcagtggt
    35641 taaaaagtgg cggcccgcgt tctgcgcgtg tggaacgggt gctcagcgag ccgcatcatc
    35701 cctccgggga gttaacggat tttcgtatcc gttaaatgca ttttaccggt ttaggcaggc
    35761 cagcaatttt ggtggcttgc tttgccggac ctttcgggaa cagtcggtac agatagcggc
    35821 tattgccttt ctcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg
    35881 gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttctg
    35941 gcgacagagt aatcccttcg ttttctgcaa tcaccaccgc cagcggctca ctccactggc
    36001 tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca
    36061 taattcttta ctaccttaat cagactggcg gcagtgtaac aaaaaacaaa gccccgcata
    36121 aagcgaggct cggtaagtaa ccctgggcga gattaatcgc ggctggcgaa gcccagaatg
    36181 ctcagcaggc tgacgaagat gttgtacagc gaaacataca ggctaaccgt ggcacgaata
    36241 tagttcgttt caccgccatg aatgatgttg ctggtttcaa acaagatagc gccagaggag
    36301 atcagaatga agaccgcgct gatcgccaga tgcagagcag gcagttgcag gaagatatta
    36361 gcaaccatac caatcagcac caccacaata cccgccatca gcataccgcc gaggaaagac
    36421 atatctttgc gggtggtcag cacatacgca gagcagcaga agaacactaa cgccgttccg
    36481 cccagtgcca tagcgattac gtcacccatt ccggcagaca gataggtgtt cagaattggt
    36541 ccgaggatat aacccagaaa accggtaaag gcgaatgcgg agataatccc ggtcggctta
    36601 ttcgccgttt tgtaggtcag gaacatcaaa ccatacatac ccaccagcgt cagaatcaga
    36661 cccggagatg gcagcatcag caccgtactg gcagttgcgg taatcgccga aaaggccagc
    36721 gtcaggctca gcagaaaata ggtattacgc agcaccttat gggtgctaag cagcgatgta
    36781 cggtcatgtg aagaactaac tatacgatcc attgagtcac tctctatgac agatgtaatt
    36841 aattaagcag cataatgata atgcgtaagg gcacccagaa gttttaccca tctttacgca
    36901 tttgatctgg aacaggttta acagcggatt atcaggtcat taggcagtga tataacgccc
    36961 tgagaatttc gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt
    37021 tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg
    37081 gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg
    37141 gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaatag ccaaaagtgc attgatagcg
    37201 tgaaacaaca gcagattgat catttcatca ccatgaattc cttctctatt tactcgttta
    37261 gcaaccggct aaacatcccc accacccggc aaaaagaaaa ataggtccat ttttatcgct
    37321 aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa
    37381 caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt cagcatctca
    37441 cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccatgt gcgcccggtg
    37501 ttctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa acattttacc
    37561 aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg
    37621 ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaata
    37681 aaccgcgcat tccggtggta tggatccacg gcctggaatg cacctgctgt accgaatctt
    37741 ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt tccctcgatt
    37801 acgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttt gaagacatca
    37861 tcgcgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg ctgggcgagc
    37921 aggggatgtt ctgtatcagc agcggacgac cgtttattga gaaactcaag cgtgccgccg
    37981 ctggagccag tgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gttcaggccg
    38041 cgcgacctaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacccatta
    38101 tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg
    38161 tgacgtttga tcgtctgccg gatgtcgaca gaatgggccg tccactgatg ttttatggtc
    38221 agcgtatcca cgataaatgc tatcgccgcg ctcacttcga cgccggagag ttcgtccaga
    38281 gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc
    38341 ctaccaccta taacgcctgt tcctccacac gctggaacga tggcgtttcc ttcccgatcc
    38401 agtcaggtca cggctgcctg ggttgtgcgg aaaatggttt ctgggatcgc ggttcgttct
    38461 acagccgcgt ggtcgatatt ccacaaatgg gtacccattc caccgccgac accgtcggtt
    38521 taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg
    38581 accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg
    38641 ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaaca atgccggacg
    38701 ccgcctggtg gtcgacccga ttacgcgcat tgaaggccac atgcgctgcg aagtgaatat
    38761 taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga
    38821 gatcattctg caagggcgtg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg
    38881 cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ccatcggtat
    38941 taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc tctggtgcca
    39001 cgatcatctg gtgcacttct atcagcttgc cgggatggat tggatcgatg tgttagatgc
    39061 gctgaaagcc gacccgcgga aaacctccga actggcgcaa agcctctcct catggccgaa
    39121 atcatcccct ggctatttct tcgacgtaca aaaccgcctg aagaaattcg ttgaaggcgg
    39181 ccagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac tgccgccaga
    39241 agccaacctg atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt
    39301 caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg tcggtgggat
    39361 gccttgcgcc attaacattg acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct
    39421 gaacctggtg cagttgatta tcacccgcac ggcggatttc attaacaacg tgatgatccc
    39481 cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca cggggctttc
    39541 tgataaatgc gtcctcagtt acggcgcgtt ccctgatatc gccaacgact ttggcgagaa
    39601 aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg tactgccagt
    39661 ggatttagtt gatccgcagc aggtgcagga gtttgttgac catgcctggt atcgttatcc
    39721 caacgatcag gttgggcgtc atccgttcga tggcattacc gacccgtggt acaaccccgg
    39781 cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg
    39841 gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cacgcacgtt
    39901 aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgta tgatgtcggc
    39961 gttgaaactg ccgctttccg gcatccagtc aacgttgggc cgcattttgt gtcgcgcgca
    40021 cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gataaactga tgaccaacct
    40081 gaaaaacggc aatcttgcca ccgcttccac ggaaaaatgg gaaccagcaa cctggccgac
    40141 agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc actgggctgc
    40201 cattcgcgat ggcaagattg atctctatca gtgcgtggtg ccgaccacct ggaacgccag
    40261 cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga acaccaaaat
    40321 ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg acccgtgcct
    40381 cgcctgttca acacacgtgc tgggcgacga cggtagcgag cttatctccg tgcaggtgcg
    40441 ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gtcactatgt
    40501 ctttgaagca cccgtgcgga tatggcactg gctgacagtg ttatgcatgg cggtgttgat
    40561 ggtcaccggt tactttatcg gcaagccgct accttccgtc agcggcgagg cgacatatct
    40621 gttctatatg ggctacatca ggttaattca cttcagcgcc gggatgattt ttaccgtggt
    40681 tttgctgatg cggatctact gggcttttgt tggcaatcgt tactcccgcg agctgtttat
    40741 cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct
    40801 gtttctggca aaacgtccga gtgccgatat aggccataac cccatcgccc aggcggcgat
    40861 gttcggctat ttcctgatgt cagtttttat gatcatcacc ggctttgcgc tgtacagcga
    40921 acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggac
    40981 gggcggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc
    41041 atttgtgatc ggccatgtct acatggcgct gcgtgaagac atcatgtccg acgacacggt
    41101 gatctccact atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga
    41161 gcgttcatga gcgagcaacg cgtggtggta atggggctgg gcaacctgct gtgggccgat
    41221 gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg gcccgaggat
    41281 gtggagattg tcgatggcgg cacccaagga ctgaacttgc tggggtatgt cgaaagcgcc
    41341 agtcatctgt tgattctcga tgccattgat tacgggctgg aacccggaac gctacgaacg
    41401 tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcaccagaac
    41461 agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc acatattgcc
    41521 ctcgtcggtc tgcaacccgc aatgctcgac gactacggcg gcagcctgag cgaactggcg
    41581 cgggagcaac tgcccgccgc ggaacaggcg gcgctggcgc agctggctgc gtggggaatt
    41641 gtgccgcaac cggctaatga atcgcgctgt ctcaattatg actgtctgtc gatggaaaat
    41701 tacgaaggca ttcgtttgcg ccagtaccgg atgacactgg aggagcaggg atgagcaacg
    41761 acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccgatcagtg
    41821 aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta ttaagcagcg
    41881 atccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa ttattgcgcg
    41941 agtttcccga ttatacatgg caggtagcga ttgctgacct tgagcagagc gaagccatcg
    42001 gcgatcgctt tggcgtcttt cgctttcccg ccacattagt atttacctgc ggaaactatc
    42061 gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcggacttg
    42121 tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc tggggccagg
    42181 aacgcaactg aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa
    42241 tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct
    42301 gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg aggtactggc
    42361 agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga
    42421 aggggaagtg tcagtgcgca ttcagcaggc tgatggtagt gaaagtgaaa ttcaggaggc
    42481 gatcttctgc ggattatggc gggtacgcag acgtcacggc gaacagttgc tggaagacaa
    42541 actggaggct ggctgcgcac cgctggcgtt gtggcaggcg gcaacgcaaa acgtcttgcc
    42601 gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggccca
    42661 tgaattactg gcacatgtac gtaaccccga cgcacagccg cacagcatta atctgacgca
    42721 attacccatc agcgaagctg accggctttt tctctcacgt ctctgtgggc cgggaaatat
    42781 tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt
    42841 ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt atgaaatctg
    42901 cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct
    42961 tagcgaggta tgccagtggc tggcggaagg tgcaccgacg taaaaagacg gtaagtatcg
    43021 ctttcaggct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa
    43081 agagggagat ctaccattaa cgggttattt ttctctcttc gcctacagga gtgcgcatgt
    43141 gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat cactttttat
    43201 ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc atttacgtgg
    43261 tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc ttcggtatca
    43321 actttgctct tggcgtggct accggcctga cgatggagtt tcagtttggt actaactggt
    43381 cattctattc caactatgtg ggcgatattt ttggcgcacc actggcgatg gaagcattaa
    43441 tggccttctt cctcgaatcc acctttgtcg ggctgttctt cttcggctgg caacggctga
    43501 ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat ctctctgcgt
    43561 tgtggatatt gaatgccaac ggttggatgc aatacccgac tggtgcgcat tttgatatcg
    43621 acaccctgcg tatggagatg accagtttca gcgagctggt ctttaacccg gtcagccagg
    43681 tgaaatttgt gcacaccgta atggcgggat acgtgaccgg ggctatgttt attatggcga
    43741 tcagcgcctg gtatttactg cgcggacggg agcgcgatgt cgcattacgc tcgtttgcca
    43801 tcggttccgt cttcggtact ctggcgatta tcggtacgct gcaactcgga gacagttctg
    43861 cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg gagtggcaaa
    43921 cagaacctgc acctgcaccg ttccatgtgg ttgcctggcc ggaacaggat caagagcgta
    43981 acgcctttgc catcaaaatt cccgcgctgc tagggatcct cgccactcac tcattagata
    44041 aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta ccctcgcttg caacgtggac
    44101 gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc
    44161 aggcattccg ggagctggaa ggcgacctgg gttacggcat gttgctctcc cgctatgcgc
    44221 cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc gcgatacctc
    44281 aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc ctgctgctac
    44341 tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag catcgctggg
    44401 tgctgaaaat ggcgctctgg agtttgccgc tgccgtggat tgcgattgaa gccggatggt
    44461 ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg acatactccg
    44521 cgcactccgc cttaaccaca ggacaactgg ctttctcact gatcatgatc gtagggcttt
    44581 acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt ctggggccga
    44641 gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa tcatgtttga
    44701 ttatgaaaca ttgcgtttca tctggtggct gctgattggc gtgatcctgg tggtctttat
    44761 gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga
    44821 tgatgaacgt cggatagtga taaacagcgt tggcgcgcac tgggaaggca atcaggtctg
    44881 gttgatcctc gcaggtgggg cattatttgc cgcctggccc agagtgtatg cagcggcgtt
    44941 ttccggcttt tatgtggcga tgattctggt gctgtgctcg ctgttcttcc gcccgctggc
    45001 ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg atgccggact
    45061 ggtcatcggc agtctggtgc cgccggtagt cttcggtatt gcattcggca acttgctgct
    45121 cggcgtgccg tttgctttca caccgcaatt acgcgtggaa tatctcggca gcttctggca
    45181 actgctgacg ccattccctt tattgtgtgg attgctcagt cttgggatgg tgattttgca
    45241 aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgcgtt cacagctggc
    45301 gaccaaacgc gccgcactac tggtgatgct gtgctttttg ctggcgggtt actggctgtg
    45361 ggttggtatt gatggctttg tgctgctcgc ccaggatgct aacggtcctt ccaatccatt
    45421 aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg aatcgcccgt
    45481 tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga tggcgattta
    45541 tcgtggtcgc ccgggttggg gatttttgat ggcgtcattg atgcaatttg gcgtgatttt
    45601 cacggcaggc atcacgctgt tcccctttgt catgccgtca agcgtgagtc cgatctccag
    45661 cctgacgttg tgggacagca cttccagtca gctgacgctg agcattatgt tggtaatcgt
    45721 gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca aaatgtgggg
    45781 gcgcatgacg acagaaactc tccgccgtaa cgaaaacgag ctgtactaag gagcaggaac
    45841 aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt
    45901 gttggtatgg ctggacccgc gtctgaaaag ttaacgaagg taagcctgat gaggcgcatt
    45961 agcgtcgcat caggcaatca ataatgtcgg atatgaaaag cggaaacata tcgatgaaag
    46021 cgatcttgat cccattctta tctcttctga ttccgttaac accgaaatct gcattcgctc
    46081 agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcac ggtgtgcgtg
    46141 caccaaccaa ggccacgcaa ctgatgcagg atgtcacccc tgacgcatgg ccaacctggc
    46201 cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcttat ctcggacatt
    46261 accaacgcca gcgtctggtg gccgacggat tgctggcaaa agagggctgc ccgcagccag
    46321 gtcaggtcgc gattattgct gatgtcgacg aacgtacccg taaaacaggc gaagccttcg
    46381 ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc
    46441 ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga
    46501 ctgacgcgat cctcagtagg gcaggagggt caattgctga ctttaccggg catcggcaaa
    46561 cggcgtttcg cgaactggaa cgggtgctta atttcccgca atcaaacttg tgccttaacc
    46621 gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga
    46681 gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat
    46741 ttctcctgca acaagcacag ggaatgccgg agccggggtg gggaaggatc accgattcac
    46801 accagtggaa caccttgcta agtctacata acgcgcagtt ttatttgctg caacgcacac
    46861 cagaggttgc ccgcagtcgc gccaccccgt tattggattt gatcatgaca gcgttggcgc
    46921 cccatccacc gcaaaaacag gtgtatggtg tgacattacc cacttcagtg ctgtttatcg
    46981 ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc
    47041 caggtcagcc ggataacacg ccgccagggg gtgaactggt gtttgaacgc tggcgtcggc
    47101 tcagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc
    47161 gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag
    47221 gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc cggttttacg caaatcgtga
    47281 atgaagtacg cataccagcg tgcgctctcc atcaagacaa ataaacattt gccgctgttg
    47341 gtccacgcag gccatttacg cggcaaaatt ccacgcgtaa tcctggtata agcacttctg
    47401 cgtcgcgggg agtgaatgcg gaaatatgga cctggacttc tttacgaccg tcggagggga
    47461 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    47521 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    47581 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg
    47641 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    47701 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    47761 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    47821 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    47881 tgatggcagc aaagacgttt tcgtccattt cactgccatc cagagcaatg aattccgcac
    47941 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gtcccgcggc
    48001 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
    48061 catgttgcca tggttcacag taccgcacat cggcattcga tgtgacggag cgaaaccctt
    48121 tgggtgcaaa gtgcattttt tgtaaatcga cgatgatcac ctttgataac gttgcgctgc
    48181 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
    48241 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
    48301 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
    48361 ggaaccaaca ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa
    48421 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
    48481 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga
    48541 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
    48601 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgatt
    48661 gcggaattaa gcgttggtac aattcctccg gcaccgggct ttgccatact cccgcataca
    48721 ttgcgtaacc aatcgccgca aacataatcc ccataaccag tagcgtcatt aaccagccag
    48781 acaacgcaaa ggctttttta tttgccgcag gtttttgcag tgaaaaggcc aatgttgagg
    48841 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
    48901 ttaatttatc gaccgggatc cgtgatgggc aactttttgc gcatttgcca caatcgatac
    48961 aactttcggc attgcgacga atcttaaacg gcgataatag cgaaaccacg cccatcagcg
    49021 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
    49081 aaacgatcat gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
    49141 cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
    49201 ataacgcgat atagagaaaa aaactcaaca gcaaatactt cacgccgcgc agaggaatat
    49261 ccagccagcg gggaaggaca aattgccgac caaacagttt gttaccgaga tcgccgatta
    49321 attccgaaag cgtaccaacc gggcataacc atgaacaaaa ggcctttttg agtaatagac
    49381 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
    49441 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
    49501 cacccggtct ggtgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
    49561 agaacgtgat ggcaatataa atattaattg ccaacagtaa taattgcgtc gctttacgcc
    49621 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
    49681 gttgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
    49741 aaatgccact attctagttg ttcttaactg gctgatattg attcaaatcg cgttcaggtc
    49801 tttcttatta accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg
    49861 ggggcagata atggctgctg ctccagttgt gcgcattgct ggctggcgct acgcattccc
    49921 agactcgagc aactgctttt tagctgatgt gcggcacgct ttattttctc gttatcctga
    49981 ctggcgcggg caatgtcgat ttcatcgaga agcggcaggg catgttgttt aaataatatc
    50041 agccattcgt ggatcttctc cgcccccatt aaatgagcat cttcattgag ttgcgataca
    50101 tccagtggtt gatcgttatt gacttgcagt tggagatagt gcgccagtaa ttgaccgagc
    50161 acttcacgcg gcaccggttt agggataatc ccgcgaaata gcgaactggt acgctgtcgc
    50221 agtgtttcgt caatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt
    50281 gccagttgtc gggcaagcgt aatgccgtcg atatccggta gatcaaaatc caccagtgcg
    50341 gcagcaaacg gttcactatt ttgcagtgtc tctaaagcct gcgcggcatt tcctacggca
    50401 acgacctgcg ccccactgct gttcaacatc tcaacggtaa tccgctgggt tagcgggtta
    50461 tcttcaatta acagcaaacg taaaccgtcc agacgcaccg cctgattgac tgtttttggc
    50521 acgggtgcag tggcaacacg taacggcacg cgtaaacaaa aacagcttcc aacctccggc
    50581 gtgctggtgg cgctcaattc gccgcccatc gcctgggcca gacggctact gatagtcagc
    50641 cccagcccgg tgccgccgcg tttgccgcta acctgaacaa atggctggaa gatttcagcc
    50701 agtttcgcgg gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgttcgcca
    50761 tcaatgcgac tacgcagaat aatgtgccct tcgtcggtga aacgcagggc gttgctcaac
    50821 aggttggtaa tgacctgacg aatacgccgc ggatcgccca ttaacgcagt cggtacatcg
    50881 tcggcaattt ctgttgccag gcgaatcggg cgacctttca cccttccgct cattaattgc
    50941 agggtacttt ccagcagcgg gcgcggttca aagggttcat cgctgaccga aacattcttg
    51001 ccgcctgctt cgatagccga ataatcgaga atatcgttga ggatggtcag caacgattca
    51061 ccagagtcgg taatcgcccg caaatcatcg cgctgggcgt taagtgcggg gttatctgcc
    51121 agcagctgtg cggtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc
    51181 gccagaaacg ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt
    51241 tctatcacca gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg
    51301 cggtggcgat tcagtgcatg aacactgctg cgaaacgcat ccatcagccg cccgatggta
    51361 tccagttccc gtacgccagc ggtttccggg aaaggggagt cgatatcacc atccagcaag
    51421 cgttgcagcg cctgcgtttg ttctgcaaga gggcgcgtga ctgagcgata aaccacgcgc
    51481 cagaggatca gaatcagtgc gcaaagagaa actaatccca gcaataacag gctgtattgc
    51541 ccgcgtgcac tggctttttc cagatgagcc agcccgtgct gattacgcag ctcaatggtg
    51601 tcgaccagct gactgacttc gctactaaac tgcgcgaatt gggcgatgtt attttgggcg
    51661 agagtttgca ggtgattgct gatttcactg tcctgctgat acagcgccag caaatcgcta
    51721 tattggctaa cggtagttaa cgttgttgct acctgagtac gaacacccgg atcttcaatg
    51781 cgtatttgcc gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc
    51841 gcatttttct ggatctgttc cagccccaga ttcatcacca tttgctgcac ccgcagggcg
    51901 ctaaggcgca gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg
    51961 agtgcacttt ctgcggcttg acgctgatgt tgttcgatca aatcgtaaat cccggcctgg
    52021 gtcgctccgg cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatttca
    52081 tcggcggcgg cgactatctg ctgactgagt tgctgttgtt gctggcgaag ttgcaaacgc
    52141 tgccccacca gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca
    52201 atagcggtgg tgtcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcaga
    52261 ctttgtgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
    52321 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
    52381 atggtaggaa tcaatgcctg agtgttgtct ttttccacct ggctgataaa gcgcaggttg
    52441 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
    52501 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
    52561 caaagcggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
    52621 ctatttttac ttctttccct tttcatgtta tcggcatttt cggctgataa cctgttgcgc
    52681 tggcatgatg cgcagcattt cacggtgcaa gcctctacgc cgcttaaagc caaacgcgca
    52741 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
    52801 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
    52861 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
    52921 gccattttgc tcggtagtag cacgacgtca tttcccgatc tgcaaaagca ggtagcaaat
    52981 ctgccggtga tcgaactggt aaatgctatt gatgctcccc acgtgaaaag ccgcgttggt
    53041 gtgccctggt ttcagatggg ctatcaacca gggcgatatc tggtgcaatg ggcgcacggt
    53101 aaaccactga acgtgctgtt gatgcccggc cccgataacg ccgggggcag taaggagatg
    53161 gtagagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctc
    53221 ggtgataacg atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccatcca
    53281 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg ggaagggcgt
    53341 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
    53401 ttgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca aggggaactg
    53461 gcggttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
    53521 ccgattttag tcatgacgcc gaaaaatgcc gaccgcgaac atatccgccg ctcgctgtca
    53581 ccagggggat ttcgtccggt ttatttttat cagcacacat cagcggctaa gaaataacct
    53641 tcgccatgtt gtgtcaccag taaatccgca ctgagtttat ggcgtaaacg acgaattaac
    53701 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagagagcat acgcagcaga
    53761 cgttcacggc tgagaatttc gcccggatta gtcacaaatg ccaccagcat ttcatactct
    53821 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
    53881 caataaccgg caaagcgata gcagttgtcc tgagtgtgcg gttgcgcctg tcgcgcgagg
    53941 tcgattcgcc agagcaaatt tttcactcgt accaccagtt cgcgcagctc cagcggtttg
    54001 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
    54061 ccggtaacca gaataatccc taccgttgag cgttctcgca gggcgcgggt taacatcagg
    54121 ccattttcat cgggcaaatt gatatccagc agaatcaaat ccaccggctg attctgcata
    54181 atttcccgca gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
    54241 taagattgta atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc
    54301 atcagagggt tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc
    54361 ctgaccagtg ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataatttta
    54421 caggggctat tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg
    54481 tactggcgct ggtcgctact gggattgtga ttggcattgc gctgattgta ttgccacacg
    54541 ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg
    54601 tgtatgaaga atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat
    54661 gccatgactg tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga
    54721 gcaacgacat ttaccagacc tttattgccc actccattga tacacctgag aaatttgaag
    54781 ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa
    54841 cctgccgctc ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg
    54901 cgcgtcagat gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta
    54961 ttgcccacca gttaccggat atgagtagtg gcttccgtaa gcagttcgat gagctgcgcg
    55021 ccagtgctaa tgatagtggt gacacgctgt actccattga tattaagccg atttatgcgg
    55081 cgaaaggcga taaagaagcg tctggttctc tgctgcctgc ttcggaagtg aaagtcctta
    55141 aacgtgacgg cgactggctg caaattgaaa tcactggctg gacggaaagt gcaggacgtc
    55201 agcgtgtact cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg
    55261 tgcagcagca ggtgaaaaca ctggagaaaa ccaccgttgc cgacaccaat accgagtgga
    55321 gcaagttgca ggccactgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga
    55381 tctgggctta tgcggattcg ctgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg
    55441 aaatatccca ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta
    55501 ccagcctcga taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg
    55561 acaccgcagg taaagctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct
    55621 ctttcaggca tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat
    55681 gctagggccg tcattgttaa cgccgcgccg tgcgactgcg gcgcaagcgg cgactgaggc
    55741 tgtcatctcg aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt
    55801 gaaggatggt cgctttgtgg cagcaaaacc gttcgaactg gataaatatc cgtcgaaaat
    55861 gatcgccgga ttgccggatc atgtacacaa cgcggcgcgt attcgttacc cgatggtacg
    55921 cgtggactgg ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt
    55981 tgtgcgcgtg agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca
    56041 gaaaactcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
    56101 ccataacgct tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttagtac
    56161 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat
    56221 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
    56281 gctgtggggc tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
    56341 tgtttatgaa tattacgagc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat
    56401 cagcatcgat ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca
    56461 cattgcggtt aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcgc atacgctgta
    56521 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt
    56581 cctgccgtat ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
    56641 actgaccggc attgatgccg aaaccattcg tgggctggca cggcagatgg cggcgaacag
    56701 aacgcagatt attgctggct ggtgcgtaca gcgtatgcag cacggtgaac agtgggcgtg
    56761 gatgattgtc gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg
    56821 ttttggctgg cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttttgagtgg
    56881 tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca aaggctacag
    56941 cagcaccatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa
    57001 ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ccggaactaa
    57061 cccgttccat cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac
    57121 ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc
    57181 tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaatcgtgg
    57241 catcatcgcc atgaaacagg tcgtgccgcc gcagttcgag gcgcgcaacg acttcgatat
    57301 tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga
    57361 aatgggctgg ctgaaacgca tctggcagga aggtgtacaa cagggcaaag gacgcggcgt
    57421 tcatctgcca gcgtttgatg acttctggaa taacaaagag tatgtcgagt ttgaccatcc
    57481 gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac
    57541 gccgagtggc ctgattgaga tctactcgaa aaccatcgct gatatgaact acgacgattg
    57601 tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ctggctcgca
    57661 gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact cgcagttatg
    57721 tgagtcggaa actctgcgtc agcaatatac cgtagcgggt aaagagccag tatttattag
    57781 cccgcaggat gccagcgcgc gcggtattcg tcacggtgat gtggtacgcg tctttaacgt
    57841 tcgtggtcag gtgttggctg gagcagtggt ttctgaccgt tatgcacccg gcgtggcgcg
    57901 aattcacgaa ggggcatggt acgatccgga caaaggcggc gagccagggg cgctgtgcaa
    57961 atacggtaac cccaacgtct tgaccatcga catcggtact tcgcagctgg cgcaggcgac
    58021 cagtgcgcac actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac
    58081 ggcgtttaac ggccccgtgg agatggtggc gcagtgcgag tatgttcccg cgtcgcaggt
    58141 gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg gctggcgcag
    58201 ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct
    58261 gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa tgggctggaa
    58321 aacagtattg ccactctgac agtacgtgac gatgcccgtc tggaactggc cgctgacttt
    58381 tgcggcctgt ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa
    58441 caggacgagc aagagattaa acgcttgtta gttgaggcgg ggatggaaac cagcggcaat
    58501 ttcaacgaac cggcagatca tctggcgatc tatctcgatt tgttgagtca tctgcatttt
    58561 tcgctgggag aggggaccgt tcctgcgcga agaatcgacg gtttgcggca aaaaacactg
    58621 acggctctgc gggaatggtt accagagttt gctgcgcgtt gtcgacagta tgacagcttt
    58681 ggtttttacg cggcactaag ccagttattg ctggtgttag tggagtgtga ctaccaaaaa
    58741 agataatgcc gtttgtaggc ctgataagac gcggcaggcg tcgcatcagg cattagtgcg
    58801 cagttgccgg atgcggcgag aacgccttat ccggcctacg ggaacagtgc tttttgtggg
    58861 cctgataaga tgcgttaagc gtcgcatcag gcattatggc gcagttgccg gatgcggcat
    58921 gaacgcctta tccggcctac gggaacagtg ctctttgtag gcctgataag acgcgttaag
    58981 cgtcgcatca ggcaacaaac tcacgggtga gccacaaacc gggaaagccg ctggcgcagc
    59041 aggcggtttt cctgcttcag gtgcgcaata tcatccatta acgtcagcgc caccgcgatc
    59101 cccggccagt ccagagccag ttcatgacgc aggcgtaccg cgcgttgcac cacaatggcg
    59161 gcatggtcgt caaataccca ggttgtctcc tggatctcac tcggttcaac cacccccaaa
    59221 ccgacaattt cattcaactc ctcttcagag atgccggtat gcaggcaaaa ttcggtaata
    59281 gtaaaagtca ccgtaacatt agccattatg ctttccccca atctttacgt ggatcaaaag
    59341 acgactgggc gtctgccagt tgctgccaca gcgcggcagt gttttcatcc ggtttcggcg
    59401 gcatcacgat tttcagtacc gcatacagat cgccggtctg ttttttgctc accagacctt
    59461 tgcctttaac gcgcaatcgt tgcccggcct ggctgccagg cgggatagtc agcaaaatgc
    59521 tttctttcag tgttggaacg gtgactttag tacccagcgc cgcttcccac gggctaaccg
    59581 gcaccactat ttccagatcc tggccgacaa tatcaaacag cggatgtggc gcaatgtgaa
    59641 tcaccagcca caggtcgcca tttggaccgc cgttttcgcc cggcgtcccc tggcctttca
    59701 ggcggatgcg ttgaccattg ccgacgcccg ccgggatctt cacattcagc gttttcggga
    59761 tttcctgttc gatcatgcca aaggcgttat aaaccggcag gttatagctg atggtacgct
    59821 tatgctcagt aagcgtttct tcgaggaaaa ccgccacttc gatttcaata tcgtggccgc
    59881 gtgtggcggg gcgttgacgg ctctggcggg catgctgacc gaaaattgac gagaagatat
    59941 cgtcaaaatc ttcggcgtta aaactctgac cgtcgccatg atggaactga cggttaaatt
    60001 gcggatcgtt gcgatgttgc cacatctgat catactcagc gcgacgttgt tcatcactta
    60061 acacttccca ggcttcggcg acctctttga agcgggcttc ggcatccggt tctttgctga
    60121 catcaggatg gtatttgcgg gccagtcgac gataggcggt cttgattgtc ttgagatcgt
    60181 ccgtcggttt cacgcccatg atggcgtaat aatcctttaa ttccatagcg ttatctcgcg
    60241 taaatcaaca caaattgaag gaacccctgt aaggtaactc ctataagtgt agggtaatcc
    60301 tcaaaatttc atatgccaac acagaatatg ttattgaaat catcgcggag gggaggttgc
    60361 ctcaaggtgg gaggaacata ttttatttac ctcactcctg aacttttggc tcatgcttac
    60421 aagttaaaca ataaggaaag gaatgcacaa ctcgactatt taaatcgtta tgcaatttaa
    60481 tgataaagac atgtgctaaa aaccattgct attagtctca cattcttttt ttattggtaa
    60541 tggtaacgcc gctgattttc tttatagcta ctgatgtaat cattagcggc gtaaacgagg
    60601 ccggggctaa tcaggcatag tttgcgtcaa aacttgcctg tttttaaagg ggaatataga
    60661 aaaacaggcg ttcaacgagc cattttgcga acctgttccc ggaaaaatga catatttctg
    60721 tcacactctt taattattga taacaaaaga ggtgtgagga atgaacaaaa cgctaatcgc
    60781 cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa ccgtaccgga
    60841 aggctatcag ctacagcaag tccttatgat gagccgtcat aacttacgtg cgccgctggc
    60901 gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggcctgaat gggacgtccc
    60961 cggtgggcag ctcaccacta aagggggcgt gctcgaagtg tatatgggcc attatatgcg
    61021 tgaatggctg gcagaacagg ggatggtgaa atcaggggaa tgcccgccac cggacaccgt
    61081 ttatgcctat gccaatagtc tgcaacgcac cgttgcgacc gcacagttct tcattaccgg
    61141 cgcattcccg gggtgtgata ttcctgttca tcaccaggaa aaaatgggca ctatggaccc
    61201 cacctttaac ccagtgatca ccgatgattc cgccgcattc agtgaacagg cggtggcggc
    61261 aatggagaaa gagctcagca aactccagct taccgacagc tacaagctac tggaacaaat
    61321 cgttaactat aaagattctc ctgcgtgtaa agagaagcaa caatgttcgc ttgtagatgg
    61381 caaaaatacc tttagcgcga agtatcaaca agaaccgggc gtttccgggc cgctgaaagt
    61441 cggcaactcg ctggtagatg cgtttacttt gcaatactac gaaggttttc cgatggatca
    61501 ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga agctgaaaaa
    61561 cggttaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg cgaaaccgct
    61621 ggtcagttat atcgacaaag ccctggtcac tgaacgcacc agtgcaccga aaatcacggt
    61681 attagttggg cacgactcca acattgcctc tctgttaacg gcgctggatt tcaaaccgta
    61741 tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt tccagcgttg
    61801 gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtacc agagtgcgga
    61861 acagttacgt aatgccgatg cgttaaccct gcaggcacct gcgcagcgcg tgacgctgga
    61921 attaagcggt tgcccgatag atgccaacgg tttttgccca atggataagt ttgatcgcgt
    61981 attgaatgaa gcggtgaaat aacagaaaac tcccccgcgt gaagcggggg agtcgctggt
    62041 taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt atcggtctta
    62101 gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc cgccattttt
    62161 tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt attgcgcaga
    62221 gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg tatcctcctg
    62281 ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct tgataacgag
    62341 caatagacag ttcttcctgg ctgggctggc gtgaaccgtc accgcctgcg atggtggttg
    62401 cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc gctgcgtagc
    62461 caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggtggat gtgatggttt
    62521 gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt ccgtatagtg
    62581 cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga ccggacatgt
    62641 taccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt tcttgcgggg
    62701 ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc ggcggcatgg
    62761 tttccggtac acgcttaacg acaacttctg cgccatccac tttgcttgca ccctcagcaa
    62821 ctgcgcgagc catcgtttca atatgtccgt acatggaata ataaagcacc agaactttag
    62881 ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac cacttattta
    62941 aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat ttataacaac
    63001 tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg cgttttttta
    63061 atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc taagaagctg
    63121 ttgcaggata ttaccaaacg cggatctgcc cgcgtcagtt cactaagctt agttccacgt
    63181 agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga tgtctatacc
    63241 cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg cagaagaccg
    63301 cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcggaggta atttcaaaaa
    63361 tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc acggtaaaag
    63421 cgacaactag ccgggctaat caatgacgca tgcatttttg tctgtagctc gtcaaaacgc
    63481 catcaccgcc ggtagccccg gcggttttct gtttatggct tctgatgaac aacttctggc
    63541 ggtggaacgt caaccaacct tctgcttaac aacgcattga gtaaaatcgc gccaaaggtg
    63601 gctgtaccga tccctcccaa cgtaaaaccg cccagtgtga gagcaaaatc acccgcaccc
    63661 agcactaagg ttactgcgac cataatcaaa ttaccgttct ggcttaaatc cacactgttt
    63721 tgtacccaga tccgtgcgcc tgcgacggca atcagcccga acacaacaat tgatgcacca
    63781 ccaatcaccg cagccggaat agtatggatc agcgcgccaa atttcggcga aaagcccaac
    63841 agcatggcga tgacggcagc ggcaacaaac accagcgtcg agtagacttt tgtcacggcc
    63901 atcacaccga tattctcggc gtaggtggtt atgccgctac cgccgacaga gccagaaagc
    63961 atcgtcgcca gcccatcgcc tacgaatgcc cgccccatat acgggtccat attgcgtccg
    64021 gtcatcccgg cgaccgcctt gagatgacct aagttttccg ccaccagaat caccgccacg
    64081 ggcgcaatca gcatcattgc ctgaccatta aaagcaggag tggaaaaatg tggcagaccg
    64141 aaccaggcag catggctgac gagagtaaaa tcgacggctt ttcccagccc taaaacgttg
    64201 gtcatcacgc catacagcag acaggcgaca attaatccta cgagaatcaa taatcgctgg
    64261 atcatgccac gggtaaatac ggccaccagc ccaatacaca gcaccgtcat taccgccatc
    64321 cagctatcaa aggccgaagc cgatacattt ttcactgcga taggcgctaa gttcaggcca
    64381 atcgccatca ctaccgcacc cgtcacaacc ggcggcatta gtcgttcaat ccagcgcgta
    64441 ccgattttca tcaccaccag gccaatgacg gtataaacca gcccacaggc gataatcccg
    64501 cccagcgcga tgctgatgtt cggattaatt ccctgaccat taaagcccgt cgcggcaatc
    64561 accacgccga caaaagccgc gctggaaccg agataactgg gcacgcgccc gccggtaata
    64621 aagaaaaaca gtaacgtgcc gatccccgac attaaaatgg aaagattggg atccagcccc
    64681 atcagaatcg gcattaacac cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt
    64741 actgccgttt gagcaaacgg caatcgttcg tccggcgcga ccacgccgct ctctgtagag
    64801 gtcgatttta actgccagtg aggaaaaccg aacattgcca tcagctgtct ccttaaggag
    64861 gttaacaagc agggcgcatc agcgcgtgat aactgcgatc gaaccacacc agcccgtagg
    64921 gtgtggcgtg acgatgaatc gcttcgatgg cgcaaaacaa aatgtcgtgg gtgccgacgc
    64981 tcaccacctg gctgatacgg cagtcaaacg aaaccagtgc cttttccagt tgcgggcatc
    65041 cggtcacacc agtctgccag cgggcggcgg caaagcggtg ttccatgggc gttttgccgc
    65101 caaaaaggtt tgaaagcggc tcctgcccgg cgctaagtgt atttacacac agcgttcgat
    65161 tttcattgaa taccggccag acggacgccc cacgattcag gcacaccagt aatgtgggcg
    65221 gcgtgtcggt cacgctgcag acggcgctgg cggtgaaccc ggcgcgcccg gctggaccgt
    65281 ccgtagtgat gatattgacc gccgcgccca tacaggacat cgcatcgcga aaggtttgtt
    65341 gatcgacaat gttcattgtt tgctccttac aacagcccgc aggcttcttc aaaggacaga
    65401 cgtggcaggc gcgcaaaaag tttgctggaa tcgccatagc cgatattaat cagcagatcg
    65461 ctcttcagcg tgctgcccgc aaaaaaggcg tcgtcaacgt attgacggtc aaagcccgac
    65521 atcgggccgg tatccagccc cagcgcacgg caggcgacga tcagataggc cgcctgcatg
    65581 gaactgttgc gaaacgctgt ttcttcggca agttgtggac tggaggtaaa ccaactgcgg
    65641 gcatcgccgt gaggaaacag ctgtggtaac cgttcataaa attcactgtc ccaggcgacg
    65701 atagcggtga cgggcgcggt cagggttttt tgcagattgc cgctggaaag cgccgggcgc
    65761 agacgttctt ttccttctgc agtgcggata aacacgatcc gggccggaga acagttagct
    65821 gatgtcggcc cccatttcat cagggcataa atctcccgta acgtctcatc gctgacgggg
    65881 ggctcccgcc agccgttgtg agtgcgggca tcggtgaaca gggtgctaag cgcacctggg
    65941 ctaacggctt cgttcatagc aattccttac agagcggctt cacggtgatg taacaggctg
    66001 gcaagcccgt tgagtaacag agcattaaac gtttcgggat cggtcacgtt gcaggcgtgt
    66061 ccgccatagc gcatcaccat tttctggcta tcgggcaggg cggcatgaag ttcactggaa
    66121 catgctgttg gcaccagcag atcgtcactg gcgcagatga tttgcaccgg gcagcggata
    66181 cgatccgcat ggtgactaaa gtcagcgcgt ttgagggcgt taagtcgacg cagtaaatta
    66241 tttttgccct gaaaatgcgc cagtgccagc gcatcttctg cctcaaggcg aggtgcgcgg
    66301 gccgccatcc agtcggcggg atagaggaac aacggctgcg cttccaccca tgcctgcgcg
    66361 ccgccgctat gtagtaactg ttcgcgaacc tgaaaacagc ggcgcgtatg ggtgtttatt
    66421 cgtagccagc cgttaacgct gaccagcaca gttaccgacg cgggataatc cagcgccagc
    66481 tgcattccca ccagcgcacc gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg
    66541 gctaccagcg cctgatgcag ttccgctgcc atctgggcga tactgtaatc ttctgccagc
    66601 gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgatcc
    66661 agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc
    66721 actacgggcg catcagcata agggggaggt gagagtgaaa gtttcatcgc ggcctcactt
    66781 ggcgatatgc gcaattgtgg cgatttccac cagcgcgtca ggttttacca gtccgcactg
    66841 aatgcagaat cgcgccggtt tgtcacccgg gaaaaactcg gcgtagattt cgttaatcgc
    66901 ggcgtaattt ttccagtcgg taataaagat gctgttgaaa gtcacatccg ccatcgtgcc
    66961 acccgccgtc tcgatgacct tgcggatggt ttccagaacg tggcgggttt gcgcctttgg
    67021 gtcatcggca aacagcacgt tgttatgttg atcaaaagcc agcgtaccgg agacatacac
    67081 cacgccatca gccagcgtgc cgggaacgaa gggggccagc ggtgcgctgc tgccagcagg
    67141 aataattacg gattttggca tcgttaaact ccttaagcga tacgagcaaa ggacgtggaa
    67201 gaaagcgcgt cgcagaacgt ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg
    67261 aacaacgcag ctttctgtgc aaattccggc cccgcctggt gtgttgcgtc ttccagcacc
    67321 acgccgaaat actccagaaa aaagccgtcg cgtagcgtcg attcgacgca gacgttggta
    67381 gcgataccgg tgaaaaccag atggcgtatt ccacggctgc gcaaaatgct gtccagcggc
    67441 gtattgaaga aaccgctgta gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc
    67501 agttcatcca ccagttgata atcccaggag cctttcgcca gcaatttccc ctgcagctgc
    67561 ggctgcttac gcatggtttt cagggcgttc gatttatgaa aattcggtga gccggggccg
    67621 ccagcctcga catactgttc atcccagcca ttttgaaacc agatgatcag catccctgcc
    67681 gctcgcgctg cggtcacggc ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca
    67741 tcaaacccgg cgagatctaa gtagccgcct ggcgtggcat aagcgttttg catatccacc
    67801 acgatcagcg cactttgctg cggatcgaag gtaatggctt ccggtcgagc ggttaaggtc
    67861 gtcatcatgc cacctcctga gtcagcgcag ggagatgggc gcggcactgc atcagtggtt
    67921 gaatgcgctc gccgaaggtt tcgattcccg acagaaaatc atcgaaggtc aacagcacgc
    67981 cttcggcacc aggcacgttt gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac
    68041 cgactaacgt ccccatattg atgtttaccg ccgaagtggg atcggccatc tgacggacgt
    68101 tggtgtcagt acctgagcgg gtatctttct gactttgttc ggttagccag cttaacgcct
    68161 cttcatccgc gcccgctttg tagtgttccc attttgcacg agcggcatcg tcggtttcgt
    68221 cggcaatcac cataaacaac acataagagc caacgtcgcg tccggtttgc tctgcggcct
    68281 gtttcatccg cgcagcggtc ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac
    68341 agaagttaaa atcggcatac cgggcggaga acgccatgcc agcgtcgctt tgcccggcgc
    68401 agatcacttt catggggaca ctcggttgcg gactgacgcg acaatcatcc atagtgaaaa
    68461 aatcgccttt aaaatcgctt ttccccgagc cccacaggtc gcgcagcacc tgaacatatt
    68521 cggtgagata gtcgtaacga cgggagaaat agtcatcgcc aggccagatc cccatctgct
    68581 catactcggg cttctgccag ccagtcacga ggttgacgcc aaaacgcccg ccagagatgg
    68641 agtcgatggt tgcggccata cgggcgacga ttgccggagg taacgttaag gtggcagctg
    68701 tcgcgtatat ctgaatgcgc gaggtcacgg ctgccagccc cgccatcaag gtgaacgact
    68761 caaggttgtg atcccagaac tcagttttgc cgccaaagcc acgcagtttg atcatcgaca
    68821 gggcgaaatc gaaatggtag tgctccgctt tttgcacaat ggctttattc agttcaaatg
    68881 tcggcatgta ctgcggcgcg tgggtcgaaa tgagccagcc gttgttgcca ataggtacga
    68941 atacgccaat tttcatcatc aacctctctt cgtctcgtaa agtgaaagtc agacggggcg
    69001 ctgcatcctg catatccttt tcagccgcgt aatggcttgt ttgcaaagcg gatgccagtt
    69061 tttaaaaagt gaatgttatt aatgtgttaa tgttaagttg tctaaaatgc ctgaaaaaaa
    69121 gtggactaaa cggtcaaaac agttgcacat aaaacatgca ttcgtgcgcg atgagagtgc
    69181 agaaggtcga ggccgggcgg gggttttgct atcctgttgc caatctacaa gaggggagag
    69241 cgcatgacgc aaggcgcagt gaaaacaacg ggtaaacgtt cgcgcgcagt gagcgcgaag
    69301 aaaaaagcga ttcttagcgc agcactggac actttttcac aattcggttt tcacggcaca
    69361 aggctggagc agatcgcgga gttggcgggt gtttcaaaaa ccaatctgct gtattacttt
    69421 ccgtcaaaag aggcgctgta tatagccgta ctgcggcaga ttctcgatat ctggctggca
    69481 ccgttaaaag cgtttcgaga agatttcgcc ccgctggcgg cgatcaaaga gtacatccgt
    69541 ctgaagctgg aagtttcccg cgattatccg caggcttcgc gcctgttctg tatggagatg
    69601 ctggcaggtg caccgctgtt aatggatgaa ctgacgggcg atttgaaggc attaattgat
    69661 gagaaatcgg cgctgattgc cggttgggtt aaaagcggca aactcgcgcc gattgatccg
    69721 cagcatttga tttttatgat ttgggcttcc actcaacatt acgccgattt tgcccctcag
    69781 gtggaggcgg tgacaggcgc gacgttgcgc gatgaggtat ttttcaatca aacggttgaa
    69841 aacgtgcagc ggattattat tgaggggatt cgaccacgct aatggtgccg gaggaaggtg
    69901 taacatcctc cggctacctg tttaaccgat cgtcattaaa ctggcgttac cgccagcggc
    69961 agcggtattc acactcagcg aacgctcgat atacagccgt tccagaagga tattgctttc
    70021 gccacgggca aaaccctgca ccgaaacaat tgcaccatcc cgcgcggcaa cggcttcaca
    70081 caatgcgcga agctgatccg aatcaccgtg gaagatcacc gcatcaaatg gttgagcggt
    70141 tatattttcc gctttcgcca gttgaatacg ttcgctgact gccgatggca atgccttcac
    70201 taactgacga tgcagcgcat tatccggcca cagcacctgg ctacccaccg ccagcacggc
    70261 ggcgagctga gtcagcgcat cctgttcatc atccgcaata cacaacacgc gctcacgcgg
    70321 cagcagcgtc caggtgttgc gttcacccgt cggccctggc aacaatcgtt gtgttcctgc
    70381 ctgcgccaac tcgccatatt gcgtacataa cgcctgcaat tctggacggt ttgccgccca
    70441 ttcccgcagt gcatttagcg gctgagtcaa tgcggctttc aactgcgcat cgaccggata
    70501 ctccgcatcc tgacgcgcga gcgtcaccgc cagcgcactt tccgggcgat tcgccagcag
    70561 acggtagaga tagagcggac cgcctgcttt cggcccggtg ccggacaatc cttcgccgcc
    70621 gaacggttgc accccaacca ctgcgcccac catattacgg ttaacgtaca gattaccgac
    70681 atgcgccgag ccagtgacct gggcgatggt ttcatcaatg cgcgtatgga cgccaagcgt
    70741 cagaccataa ccggaagcgt taatctgctc gatcagctct gggagctggt tacggttgta
    70801 acgcaccaca tgcaacaccg gaccaaagac ctctttttgt aattcggcaa agtcatccag
    70861 ttcgatcagc gtcggagcga caaaggtgcc gctttgccat tcacgggcat cttcgctgtt
    70921 ttcccgcacc gcctggaaca cctgacggcc tttactacgc atggtctgaa tatgacgctc
    70981 gatattggct ttcgcttcgc tatcaatcac cggaccgata tcggtggtca ggcgacccgg
    71041 attacccatc cggcattcgg ccattgcgcc gcgcagcatt ttcagcgtgt ggtcggcaat
    71101 ctcatcttgc aggcacagca cgcgcagtgc cgaacaacgc tgacctgcac tgtcgaacgc
    71161 cgaggccagc acatctatta cgacttgttc ggtcagtgct gaagaatcga caatcatcgc
    71221 gttcatgccg ccggtttcag cgatgagcgg aatagggcga ccctgagcgt ccaggcggct
    71281 ggcgatattg cgctgcagta acgtagcgac ttcggttgaa ccggtaaaca tcaccccgcg
    71341 cacgcgatca tcacccgtca gttgcgcgcc cacggtttca ccccgacctg gcagcaattg
    71401 caccacgcct ggcggtacac ccgcttccag caaaatggcg atcccttgcg cggcaatcag
    71461 cggcgtttgt tctgccggtt ttgccagcac gctgttacct gccgccagtg cggcagcgat
    71521 ctgtccggtg aaaatagcca gcgggaagtt ccacggactg atacatacca caggccctaa
    71581 tggacggtgg gtttcgttag cgaaatcatc ccgcacctgt ccggcatagt agtggagaaa
    71641 atcgaccgct tcgcgcactt cggcaatggc gttactgaag gtttttccgg cttcacgcac
    71701 cagaatacca atcagttgct gcatctggct ttccattagc acggcagcgc gatgcaaaat
    71761 tgccgcgcgt tcagccggag gagtggcaaa ccagattggc gcgttattaa ccgcactttc
    71821 cagcgcctgt tctacttcac gcggcgtggc ttcacgcaca tagcccacaa tatctttcgg
    71881 ttccgcaggg ttaataacgg gcgacatctc acctgccgct accggttgtt ccagcattgg
    71941 caaggactgc catttttgca gtgcactatt gagcagggct gaagagagcg aggccagacg
    72001 gtgttcgtta gcgagatcca gccctgccga gttgtcgcgc ccgtgaccgt aaagatcgcg
    72061 cggcagggga attttcggat gcggtaatcc agtttgccct tcctgttgcg ccagtttttc
    72121 tacagcagtg actggatcgg cgaccagttc atccagtggc aaagaggtgt cggcaatacg
    72181 gttaacaaac gaggtgttag caccgttttc cagcaggcgg cgcaccagat atgccagcag
    72241 cgtttcatgt gtaccgaccg gagcataaat acgacacgga cggttaagtt tgccgtcggc
    72301 aactttcccg gtgacctgct catacagtgg ctcgcccata ccatgcaggc actggaactc
    72361 gtactgaccc gggtagtagt tttgacccgc cagttgataa attgccgcca gcgtatgggc
    72421 gttgtgcgtc gcgaactgcg ggtagattag atttggcacc gccagcagtt ttttcgcaca
    72481 ggcaagataa gaaacgtcgg tatacacctt gcgggtataa accggataac cttcaaggcc
    72541 gtccatctgt gcacgcttga tttcactatc ccagtacgcg cctttcacca ggcgaatcat
    72601 cagacggcga cggctgcggg tggcgagatc aatcaggtaa tcgatcacca acgggcagcg
    72661 tttttgataa gcctgaataa caaaaccgat gccgttccag cctgccagtt caggctcgaa
    72721 acagagtttt tccagcagat cgagggagat ctccaggcga tcggcctctt cggcatcgat
    72781 gttaatacca atatcgtact gacgcgccag cagggtgagt gatttcagac gcgggtaaag
    72841 ctcttccatt acccggtcat actgggcgcg gctataacgc ggatgtagcg ccgacagctt
    72901 gattgaaatg cccggacctt catagatgcc acgaccgtta gacgctttac caatggcgtg
    72961 aatcgcctgc tgataggaaa ccatatacgc ctgtgcgtct gcggcagtca gtgcggcttc
    73021 gcccagcata tcgtaagagt aacggaaacc tttctcttcc agtttgcggg cattggctaa
    73081 cgcttccgcg atggtttcgc ccgtgacgaa ctgctcgccc atcaggcgca tcgccatatc
    73141 cacacctttg cggatcagcg gttcaccgct cttaccgata atgcggttca gcgagcggga
    73201 gaggctggct tcgttatggg tggaaaccag tttgccggta aacagcagcc cccaggtagc
    73261 ggcattaaca aacagggacg ggctacgacc aatgtgtgac tgccagttac cgttactgat
    73321 tttgtcgcga attaacgcgt cacgggtggc tttgtcggga atacgcaaca acgcttccgc
    73381 cagacacatc agcgccacgc cttcctgcga tgacaacgaa aactcctgca ataacccctg
    73441 aaccatacct gcgcgaccac tggcgttttt ttgattacgc agtttatcgg ccagttgata
    73501 cgccagtttg tgcgcctgtt cagcaactgg ctgcggcagg cgagcttgtt ccagcagcat
    73561 agaaaccgct tcggtttccg ggcggcgata ggccgcggtg atcgcggcgc gggaaaccga
    73621 ctggggcaat atttgttcgg caaagtcgag gaaaggctgg tgtggttcct ctgccggagt
    73681 cggtgcttca tcgctctcat tggccgcgcc agaaagcagc gcaggtagct ccgggagagt
    73741 atcgctgttt tccagttgtt caagataaga aaaaatcgcc tgtttaatta accagtgtgg
    73801 tgtgcgatcg atacgtgtcg cagcagactt aatacgctca cgcgtcgcgt cgtccagctt
    73861 aacccccatg gtggtggttc ccatgccatt actcctgttg ttctgaaagg tgcaacttaa
    73921 cgttatcgtg aaatatccat gatgttgcaa ctttgtgcaa ccatgttaaa tgtgacctgc
    73981 gtagcaagct taaaaatgaa tgaaatgtta ataaacgaaa tcgatatgac agggattaaa
    74041 aaaataactc agactttttc tctgcggcag ttaacatttt tgaaaggtgc aaccgcaaaa
    74101 aatgtgagag agtgcaacct gatgaaaaat agtgtcgctg agcactaaaa tttaatgtaa
    74161 atggtgtgtt aaatcgattg tgaataacca gcgcttccgg caggatacgg tcgccctggt
    74221 aaaacataaa ctctgttacc ccgttccggt ggcagatata acggcaagtt tcgacattgc
    74281 cgataataat tttttggaga ctttagatgg ctattagcac accgatgttg gtgacatttt
    74341 gtgtctatat ctttggcatg atattgattg ggtttatcgc ctggcgatca acgaaaaact
    74401 tcgatgacta tattctgggc ggtcgtagtc ttgggccatt cgtgacggca ttatcggcgg
    74461 gtgcgtcgga tatgagcggc tggctgttaa tggggttgcc tggcgctgtt tttctttccg
    74521 ggatttccga aagctggatc gccattggcc tgacattagg cgcgtggatc aactggaagc
    74581 tggtggccgg gcggttgcgt gtgcataccg aatacaacaa taacgcctta acattgccgg
    74641 attatttcac cgggcgcttt gaagataaaa gccgcatttt gcgcattatc tccgcgctgg
    74701 ttattttgct gttcttcacc atttattgcg cttcgggcat tgtggcaggc gcgcgtctgt
    74761 ttgaaagtac ctttggcatg agctacgaaa cggctctgtg ggcgggggct gcggcgacga
    74821 tcctttacac ctttattggc ggtttcctcg cggtgagctg gactgacact gtacaggcca
    74881 gtctgatgat ttttgccctg atcctgacgc cggttatcgt gatcattagc gtcggtggct
    74941 ttggcgactc gctggaagtg attaaacaaa agagcataga aaacgtggat atgctcaaag
    75001 ggttgaattt tgtcgccatt atctcgctga tgggttgggg gctgggttac ttcgggcagc
    75061 cgcacatcct ggcgcgtttt atggcggcag attctcacca cagcattgtc catgcgcgtc
    75121 gtattagtat gacctggatg atcctctgcc tggcaggggc ggtggctgtt ggtttctttg
    75181 gtatcgctta ctttaacggg cacccggcgg tagcaggtgc ggtaaaccag aacgctgaac
    75241 gcgtgtttat tgaactggcg caaattctgt ttaacccgtg gattgccggg attttgctgt
    75301 cggcgattct ggcggcggta atgtcaacgt taagttgcca gctgctggta tgttccagtg
    75361 caattaccga agatctatac aaagcatttc tgcgtaaaca ggccagccag aaagagctgg
    75421 tgtgggtagg gcgtgtgatg gtgctggtgg tggcgctggt ggcgattgcg ctggcggcaa
    75481 acccggaaaa ccgcgtgctg ggcttagtga gctacgcgtg ggcaggcttt ggcgcggcgt
    75541 ttggtccggt ggtgctgttc tcggtgatgt ggtcacgcat gacgcgcaac ggtgcgctgg
    75601 cggggatgat catcggtgcg ctgacggtta tcgtctggaa acagttcggc tggctgggtc
    75661 tgtacgaaat tattccgggc tttatcttcg gcagtatcgg gattgtagtg tttagtttgc
    75721 tgggtaaagc gccgtcagcg gcgatgcaaa aacgctttgc cgaggccgat gcgcactatc
    75781 attcggctcc gccgtcacgg ttgcaggaag ggtaagggaa ggcgttttac cgaaaatttc
    75841 acgtcataac aggaagcagg cagagggcaa tcagtgattg ccctctgcgt tttttctcaa
    75901 aatattttta catcttctca tcattatcac tacatttcac ttgtattttt cttcatcgta
    75961 atgataatcg ctatcactgc gatttacttt tctttgcata gattgactaa gaaaaacgtt
    76021 taagggtggg tggcatgttt gttccgtttc tcattatgtt gcgcgaagga cttgaagccg
    76081 cgctgattgt cagtttgatt gccagctatc ttaagcgtac ccagcgaggc cgatggattg
    76141 gtgtaatgtg gattggcgtg ttgcttgccg ctgcgttgtg cctgggcttg ggtatcttca
    76201 ttaacgaaac caccggcgaa tttccgcaaa aagaacagga actgtttgaa ggcattgtgg
    76261 cggtgatcgc cgtggtgatc cttacctgga tggttttctg gatgcgcaaa gtgtcgcgca
    76321 acgtcaaagt acaactggag caggcggtcg atagcgcatt gcagcgtgga aatcatcatg
    76381 gctgggcgct ggtgatgatg gtcttttttg ccgttgcaag ggaagggctg gagtcggtct
    76441 ttttcctgct ggcggcattt caacaagatg tcggggtctg gccgccgctg ggtgcaatgc
    76501 tcggtcttgc tacagccgtg gtgcttggct tcctgctcta ctggggcggt attcgcctca
    76561 atcttggtgc attttttaaa tggaccagtc tgtttattct cttcgtcgcc gcagggctgg
    76621 cagctggtgc cattcgcgca tttcatgaag ccggattgtg gaaccacttt caggaaatcg
    76681 ccttcgatat gagcgcggtg ctctcaactc actcgctgtt tggcacactg atggaaggga
    76741 tttttggcta tcaggaagca ccgagcgtca gcgaagtcgc cgtctggttt atttatctca
    76801 tcccggcgct ggtggcattt gttctgccac cacgcgcagg ggcgactgcg tcccgctccg
    76861 tgtagcaaat acgacgcaaa ctcttgctta gttacaacat actttaaagg gatagtctcg
    76921 tcatgaccat taacttccgt cgtaacgcat tgcagttgag cgtggctgcg ctgttctctt
    76981 ctgcttttat ggctaacgcc gccgatattc cgcaagtcaa agtcactgtg acagataagc
    77041 agtgcgagcc gatgaccatt acggttaacg ccgggaaaac acagttcatt attcagaacc
    77101 acagccagaa ggcgctggag tgggagatcc tcaaaggcgt gatggtggtg gaagagcggg
    77161 aaaatatcgc ccctggtttt agccagaaaa tgacggcgaa tttacagcct ggcgaatacg
    77221 atatgacctg cggtctgctg actaacccga aagggaagtt gatcgtcaaa ggtgaagcaa
    77281 cggcggatgc ggcgcaaagt gatgcgctgt taagtcttgg tggtgcaatt actgcatata
    77341 aagcgtatgt catggcggaa accacgcaac tggtgaccga caccaaagcc tttaccgacg
    77401 ctattaaagc aggcgatatc gaaaaagcga aagcactgta tgcgccgacg cggcagcact
    77461 atgagcgcat cgaaccgatt gctgaactgt tctccgatct ggatggcagc attgacgccc
    77521 gtgaagatga ttacgagcaa aaagccgccg atccaaaatt caccggtttc caccgtctgg
    77581 aaaaagcatt gtttggcgat aacaccacca aagggatgga taagtatgct gaccagcttt
    77641 ataccgatgt ggtcgatttg caaaaacgca tcagtgaact ggctttccct ccttcaaaag
    77701 tggtcggcgg tgcagccgga ctgattgagg aagtggcggc cagcaaaatc agcggtgaag
    77761 aagatcgcta cagccacacc gatctgtggg atttccaggc taacgttgaa ggctcgcaga
    77821 aaattgtcga tctgctgcgt ccacaactac aaaaagcgaa tccggaactg ttggcaaaag
    77881 tcgatgccaa cttcaaaaag gtcgatacca ttctggcgaa ataccgcact aaagagggtt
    77941 ttgaaaatta cgacaaactg accgatgccg accggaacgc actgaaagga ccgattactg
    78001 cgctggcgga agatctggcg caacttcgtg gtgtgctggg gctggactaa gcgttatgca
    78061 gtatgaagat gaaaacggcg tgaatgaacc gtcacgccga cgtttactga aagggattgg
    78121 tgcgctggcg ctggcgggaa gttgtccggt tgctcatgca caaaaaacgc aaagtgcgcc
    78181 gggtacgctt tcaccggatg cacgcaatga gaaacagccg ttttatggtg agcatcaggc
    78241 agggatcctg acaccacaac aggctgcaat gatgctggtg gcgtttgatg tgcttgccag
    78301 cgataaagcc gatcttgaac ggttgtttcg cttgttgact cagcgttttg cttttctgac
    78361 tcagggcgga gcggcaccag aaacgccaaa tccgcgcctg ccaccactcg attccggcat
    78421 tcttggcggc tacattgcgc ccgataatct caccatcacg ttatcggtgg gtcactcatt
    78481 gtttgatgag cgctttggcc ttgcgccaca gatgccaaaa aagttgcaga agatgacgcg
    78541 tttccccaac gactcgctgg atgcggcgtt atgtcatggt gatgtgttgc tacaaatttg
    78601 cgccaacacc caggacacgg tgatccatgc gctgcgcgat atcatcaaac acacgccgga
    78661 tttgctcagc gtgcgctgga agcgggaagg gtttatttcc gaccacgcag cgcgtagtaa
    78721 aggcaaagag acgccgataa atttgctggg ctttaaagac ggcaccgcca atcccgatag
    78781 ccagaatgcg aagttgatgc aaaaagtggt gtgggtgacg gcagatcagc aggagcctgc
    78841 gtggacaatc ggtggcagct atcaggcagt acgcttgatt cagtttcgag tggaattttg
    78901 ggacagaacg ccgctgaaag agcagcagac gatatttggt cgtgataagc aaaccggtgc
    78961 gccgctgggc atgcagcatg agcatgatgt tcccgattac gctagcgatc cggaagggaa
    79021 ggtgattgcg ctggacagcc atatccggct ggcgaatccc cgcacggcgg agagcgagtc
    79081 cagcctgatg ctgcgtcgtg gctacagtta ttcgttgggc gtcaccaatt ccgggcaact
    79141 cgatatgggg ctactgtttg tctgctacca acacgatctg gaaaagggct tcctgacagt
    79201 acaaaaaagg ttgaatggcg aggcgctgga ggaatatgtc aaacctatcg gtggcggcta
    79261 tttctttgcg ctgccgggtg tgaaggacgc gaacgattat ctcggaagcg cattattgcg
    79321 ggtttaatgt ttttaggcgt ataaggcgtt tacgccgcac caggcgttcg tgcgctgatg
    79381 cgacgcttgc gcgtcttatc atgcctacaa tcggtgcggg tcggtaggcc ggataaggcg
    79441 ttcacgccgc atccggcaag cttttaccag taccgcgaat cctgctgaaa gcacacagct
    79501 tttttcatca ctgtcatcac tctgtcatct ttccagtaga aactaatgtc actgaaatgg
    79561 tgttttatgg ttaaatataa gtgaatatat tgttgcaatg aatgcgaaat ctgttgtact
    79621 tattaagtag cagcggaagt tcccggcagt gatagtcagt cactatggag atcgcggatg
    79681 gtaacgtcct gtactggaca tgttttagag aatcagcgcg ccaccactcg cggagatttc
    79741 tcctccggta gtcatctcgt cactttgtat tttcaacctc atcctttctt ttcatgtgtt
    79801 accgacgccg taaacggtgc gcgtagccgt ttttcggcat tttatccaaa agcaaactat
    79861 ggcttacaag gaagccaacc ctctgatgtt cgtgcgcata atcgcgctgc caacggcgcg
    79921 tgtgatgaat acaaacaact caaggtgctc tccatgggaa gacaaaaagc agtgatcaaa
    79981 gctcgtcgcg aggcaaaacg tgtgctgaga cgggattcgc gcagccataa gcagcgtgaa
    80041 gaagaatcgg tcacctcgct tgtgcaaatg agcggcgtag aagccattgg tatggcccgc
    80101 gacagtcgcg atacctcgcc catcctcgcg cgaaatgaag cgcaattgca ctatctgaag
    80161 gctattgaga gtaagcagct gatatttgcc acgggtgaag ccgggtgcgg aaaaacctgg
    80221 atcagcgcag caaaagcggc agaggccctg atacataagg atgttgacag gattatcgtc
    80281 acccgtccag ttctgcaagc cgatgaagat cttggtttct tacctggtga tatcgcagaa
    80341 aagtttgctc cctattttcg cccggtctac gacgtgctgg tccgtcgctt aggggcttcc
    80401 tttatgcagt actgcctgcg accggaaata gggaaggtgg aaattgcgcc gttcgcctat
    80461 atgcgtggac gtacctttga aaatgcagtc gtcattcttg acgaggcgca gaatgtgact
    80521 gccgcgcaaa tgaaaatgtt tttaacccgc ctcggggaga acgtgacggt tatcgtcaac
    80581 ggtgatatca cgcaatgcga tttgcctcgc ggcgtgcgct ccggattaag tgacgcgctg
    80641 gaacgtttcg aagaggatga aatggtcggg atcgtccgat tcggtaaaga ggattgcgta
    80701 cgttcggcac tttgccaacg tacgctacat gcatacagtt aagtgtatta tcggtgcaga
    80761 gcccgggcga accgggcttt gttttgggtg tttatacccg gactagcgtt ttttctgaaa
    80821 ccaccatttt tatttgtccc tggctggtga aatgtacgct catcctgtgg cttttttgta
    80881 gttgctgata aagctcatca ggtattgcta agctctctgc atattcttgc ggcgtatatt
    80941 ggtatgctgc atgatgctgc tgtttttgaa aacgcagctt attgtacaac gcccagacga
    81001 ttaacacgac ggcattcacc actgccagca aaaaatagaa ctgaagtcgg cttctggcct
    81061 cgctttgcca gaaataaccc gtcagcagat caatggcgaa taagaatatg aataaagcaa
    81121 ataatgtcca caagatagtt gtggcaacat aatcaaccag taaacgtact ggcgattgtc
    81181 gggtcgtaat aattagattg ttcatattaa cctctcagaa tcccacgatc gggacttacc
    81241 caacgagcgc gttgtttttt aggcatcaac atgactcgtg taaatgatac caacgtcgtt
    81301 gccaggctta gcatccagaa aataaccggg aaccaaataa tccagaaaag cgatgaagcc
    81361 agattgtgct catagcgatt ctcaatcacc agactgacaa taaattgcag taaacataac
    81421 gtacacaata atattccggc agtatgtgtc gctgcgatat gtgtcaattc aatatttaac
    81481 ggtacacctg caagttgggc tgcgtaaata atgaaaccga ccaggcaggt aaaggcccat
    81541 attgtcgtca gacagtattc aaaaaacagt ggccacattc gaaagttttc tttgcgccac
    81601 aaccttgtca tatttttgag gaatacttct gcaccgccct gagcccagcg caggcgctga
    81661 ttccacagcc cttttaacgt ttcaggcatt aatatccagc acagcgcccg tggctcgtaa
    81721 aaaatcgtcc actgattcaa ctgcagcttc cagctaatat caatatcttc ggtgatcata
    81781 tcgtcactcc agtaacccac ttctgccagg gcgctgcgac gaaatgcggc aataacaccg
    81841 gaaacggtaa atacgtttcc atagatacgc tgggtccgct tgatcaaacc aataattgag
    81901 gaatactcgc caacctgaat tttacccacc agggtagaac gtgtgcgaat acgaggatta
    81961 ccggttacgg cacccacacg cgggttgtac aacatcggtt ccacaatata tgccgctgca
    82021 tcgcggtcta ataacgcgtc gccatcaatg cacaccagat attcactttt cgccgcggca
    82081 gctccggttt taagcgcaat ggctttcccc tggttttgcg ccagatgaat gacccgcaga
    82141 cggggaattt gtgcagccat gcgatccagg atggcacggg ttttgtctgt tgaaccatca
    82201 tttacggcaa taacttcaat gttctcataa cgctgtgcta aagcggcatg tatggtttct
    82261 tcaacgtttt tctcctcatt aaaacaggga atgataatgg agatagatgg attatctttc
    82321 aactgggggg ctggtgcgtt ttctccccac ggccagtggc gttcacgata gacccagaaa
    82381 tagacgccgc caataatcca cataatggac ataaaaaacg gccagaagaa aacgaacctc
    82441 atcatcagtt caccagagtg aaagtacgct acgcataggg gtatgcataa caccagacat
    82501 aatataaaaa acgatacgat gcgattaatc atttttcgga taccaggctg ttgaaaactc
    82561 aggacgaata acgtctattt caggttggtt atgcagaaaa ttgtcgggat aataaccata
    82621 atttttcact ccattcagtt gtaataggct catccagtga gcgagttgtt gtgaagaaat
    82681 agcctgatgc tgaccatttt tctgccagtt ttgtgcctgt aattctaaaa ttgatttgtc
    82741 tttagcctgc gggatgcttt tcatctgatt ggtcaattgt attaaccatt gatcagccga
    82801 tttttctgtg acgccttcca tataaggcat agccataata gcggtccagt catagctttt
    82861 taggaaatca gcataattct gtgcaaacca ggcttcactt tcaggttgta ttaccggaag
    82921 tgcaaaaata tttcgtgcag ttttaacatg tggaccacga atggctttta cgcgcgcact
    82981 aagttctaaa gtgaagtcag ctaacgcacg acttttaaag cgggtccact gtttaaattg
    83041 ctccgggttt tgtcgaattt cgctcagact cccgctaaag ccagcttgct gataagccgc
    83101 gatagccgct gcactggcat cttcataatc tgaaagcaaa gcatcatcgt ggaacaatat
    83161 gccatcaaaa gcagcatgtc cggcaagatc atcatataac ataccaactt gtgctctgac
    83221 cctgtcatcg aaaggagaga gacggcggta ttgttcagga tgaatttgtg cttttttctc
    83281 cccagttggt aagtatttta ctcgcgttaa tgtgggatct aaatcccagc ttaataccgg
    83341 catccacgca tagatgttta cacctgagcg ggtacgcaat tgccaggcaa cccgactaaa
    83401 aatatctgct ttcattggta gcaaacgatt tggaaaccag acctctttaa ccagcccatc
    83461 accatcggga tcagcaaatg cttgcaaata cacggtagat atttgcatat cttttacccg
    83521 ctgaattagc acatcaatat tgcgatccat ttgctggcgg ttttcgtcat aaacgtaatc
    83581 aagatcgata tgcattaccc gttgtagtgg tttttcctgt acggtaataa tttgctgggc
    83641 aaactctttt aatgagggat tattggcgat taatactcgc ggaatggaat ctaattgcga
    83701 cgcatttgcc aaacctgatt caagggtgaa gaacatgtca taaccgagtt tttttaattc
    83761 cgctattgct atgccattcg cttcgccata aggccaaaca aaaacgtgtg gatttacctc
    83821 agcctttgta cgcaggtatt ccgtcatttt tacagcatcc aggcgaattc tttcccggta
    83881 ttctgctgcg gtttcatacc gtgcgtggtc agtaaaatat gcacgattta catatgcagg
    83941 caataagctg ccggtggcat tagcctgaat accgtagtga gaatcccatg tatgagaagc
    84001 gacctcaacg agccgggaac gcgcaacttc tcgcacttgt tgccacgtgg caaaatattc
    84061 tcgactgacc atctcatcgc caaatttcac ttgtttatcc gctggagtat cgacccaact
    84121 gccgacggga gcccatacag ccggccactg gaaggcctga agaattggga agacacgggt
    84181 ataaaaactc tggtagccgt catcaaaagt cagcactact gctttttccg gtagcggttt
    84241 tcctcctcga tgtgcttcac gaatttgagc aatactgacc ggttgataac cgttctcgcg
    84301 cagccaggca aattgttcac gcagtgctga tgttcgcact gacataaaac gctggtcggc
    84361 agcttcgtct tcaacgttat gccatgaaat caccacaaaa ccattatgtg gccacggttg
    84421 ctcggcgagt aaagattcgc gatcctgtgg cggtataaat gatgttcttg actggctaat
    84481 gcacgcggtg agcataatta tactcaccag catcaggaga tatttatttc cattacgtaa
    84541 catatttatc cttaaaatct gaatgtcata tcgaattcaa cgtataagtt gtgttctctg
    84601 tcaccgtcat aaggtcgttt ttcccagcgt agcgttgcgc cagcatcaat cacgtcattc
    84661 cagctaatac gttgcccgta gccgagttgg gtgacgacat ccgtgccata atgtttttgc
    84721 caggaggcac caacacctgc gctgaatatt tgctcccagc tattttcata gcttcgccat
    84781 aacaaatgac ttgcctcaaa tgccggaaca atatcgaaca tttttatagg gttgtagtat
    84841 ggggtgtcgt gttctgtatt ttgttcgtaa tacagattgg gtaggaaatc gacaatcaaa
    84901 tatggtgaag accagatgcg ttcttgaccc tcaattgaga cttcatgacg ctggttactg
    84961 tcggaaaaat cagtgaaagc ccaggagaca ccgtacttac gccgctcatt ttgataccag
    85021 cgaacataag cctgtgcgct gttgcctgta acaccatttt tcattgcccg taatggaacg
    85081 cggtgagaga ggcgttccag ttgcgaacca atacgccagt tatcattaaa atcataccag
    85141 ccagacaggc gcgcgccggg tttatgctca tgattgaaaa agcgttcagc gtactccgcc
    85201 tcgagccaga tattacgtga ccgccactca acacctgcca gccagtcgcg aacaatccct
    85261 tttccttcgc taaattgtcc atcggcataa ccgaatccag caaaaccgcg ccagttatca
    85321 ttcagcggtg gtgaataaac gatggtggtt aagtcgacat catgtttgcc actatctgga
    85381 ccttcggcat caattcctgt tgagccagcg atacgaagct ctgcaagatt atgtacatca
    85441 accgcacgtt ttaatcgtac aacgccggga tcttgcggtt cacgttcgac aacatcgtgg
    85501 gttaagacag ctgcctgctg ccattcttgt aacgttaatg ctgtccaggc ttgttcaacc
    85561 tccagattaa tattacgtgg ctcgatcact tctgcttttt ttaattcatt ttctgccgca
    85621 cgaggccaac cgcgggcttg taacacactc gcgtaatcga tgcgcagtcc ctgatttcct
    85681 ggtgcgttat aagcaagttc tctggttgtt atttcagctt gaggaagatc attactatat
    85741 tttgctacgg ttgagagaaa cgaatgcccc tgtaaccagg tatcattcgg gatgctcgta
    85801 ggcgtgccca ttagccgaag gaaaggcggc gaagtattaa tggtatgttt ggtgacagtt
    85861 agcgcgccgg gataattttc actctccagg tggctgtaaa agagatccgc aagttcttca
    85921 tcggataaat ccgtggcaat ggtttccttg ttataaaaga gctcggtcat tattgactgt
    85981 gcttttttcg gctgctgatc tttgagataa gccgacgcaa cccaatatag cgcccagggc
    86041 ggaataattt gccccgtctt ttttaaacgc tgatagtgag aaataacgtc tctataacga
    86101 tcgcgagtta ataacgcgcc aagatgatca acctgaatac gctggtactg ggcagtgcgg
    86161 tctgggttat cgtgccacag aatttctaat gcagcgtatt gggcgagggc gcgatcggca
    86221 atggcataac gttcattttc actgcgcgta ggcataaacg acagtctgac cagttcggca
    86281 tgaatatcag cgcgaatatc tggcgttaaa ttggcatcgt caatcgcggc agctagttga
    86341 ttattacgta atgccagcac gtattctgtg ggatattgtt gcttagatgc attttcaggt
    86401 aatgactctg tcatcgcccg taattcatcc tgataacgcc ccgtcagttt atagatatag
    86461 gcttctgcga gtaaattggc tttgtccggt gctccagatt taagctgctt aagtttaaac
    86521 agcgcagtat catagtgacc cgcatctgcc agggttaaaa tttgtccccg ttgataatcc
    86581 ttattttgcg gctccagaga gagcgccttt tgccacagtg taagcgagtt ttgccattgt
    86641 tgcaggttac gataagcgac ggcgacagct gcataaccac gcgctggtaa ttgctgatga
    86701 cgatagcggt tgtaaacggt aataacctgt ttatcttgcc cggcccataa ggcaatctgt
    86761 aaccagtcag caatttggtt attactgagt gttgattttt gtgcaaacca tgacaaagct
    86821 ggctgagtat ttcctttgcg agcttcaata atcaatgcat cataggcgtt attaacagca
    86881 ctcttcgccg cgggactcgc tgctaaaaat gcagcagtaa gaattttcaa agcccatttg
    86941 gttttcgggc acctttttct gctacttgaa tacatcctgt attactccat gtattgccaa
    87001 aatctctctc tgtatctaat tacaggtaac tgaaaagaaa gatatttttg catctcataa
    87061 tccgttatta aacgcggaag agagacgtga attgttgatg atgagaagaa gaaatgatga
    87121 gcagagtgtc catataaaat ccttttctcg cccgaaaatc cattccaatg atgaggatct
    87181 tcaggaatac ggcataaatc ccaatacctt tttcaaaata aattaggatt aaaataatta
    87241 aatcagtaaa ttccgatgca tgatttcgga tttttcgaaa gtcctgatga aaggcagcgc
    87301 tactgcactc acggatttaa ttgttattcc taatgtatct att
//
LOCUS       DACADA010000002        85575 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.002, whole genome shotgun sequence.
ACCESSION   DACADA010000002 DACADA010000000
VERSION     DACADA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.002"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..476
                     /gene="agaB"
                     /locus_tag="ISB17_000083"
     CDS             <1..476
                     /gene="agaB"
                     /locus_tag="ISB17_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP1978074.1"
                     /translation="SSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDI
                     QQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLK
                     DVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            515..1318
                     /locus_tag="ISB17_000084"
     CDS             515..1318
                     /locus_tag="ISB17_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine permease IIC component 1"
                     /protein_id="HAP1978075.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1308..2099
                     /gene="agaD"
                     /locus_tag="ISB17_000085"
     CDS             1308..2099
                     /gene="agaD"
                     /locus_tag="ISB17_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP1978076.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKNDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2100..2867
                     /locus_tag="ISB17_000086"
     CDS             2100..2867
                     /locus_tag="ISB17_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAP1978077.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(2917..3780)
                     /gene="rsmI"
                     /locus_tag="ISB17_000087"
     CDS             complement(2917..3780)
                     /gene="rsmI"
                     /locus_tag="ISB17_000087"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP1978078.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3845..5881
                     /locus_tag="ISB17_000088"
     CDS             3845..5881
                     /locus_tag="ISB17_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAP1978079.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            5839..6234
                     /locus_tag="ISB17_000089"
     CDS             5839..6234
                     /locus_tag="ISB17_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAP1978080.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6254..6844
                     /gene="diaA"
                     /locus_tag="ISB17_000090"
     CDS             6254..6844
                     /gene="diaA"
                     /locus_tag="ISB17_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAP1978081.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6854..7429
                     /gene="yraP"
                     /locus_tag="ISB17_000091"
     CDS             6854..7429
                     /gene="yraP"
                     /locus_tag="ISB17_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAP1978082.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7542..8582)
                     /locus_tag="ISB17_000092"
     CDS             complement(7542..8582)
                     /locus_tag="ISB17_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1978083.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8655..9290)
                     /locus_tag="ISB17_000093"
     CDS             complement(8655..9290)
                     /locus_tag="ISB17_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP1978084.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9418..9936
                     /gene="yhbO"
                     /locus_tag="ISB17_000094"
     CDS             9418..9936
                     /gene="yhbO"
                     /locus_tag="ISB17_000094"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAP1978085.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9916..10359)
                     /locus_tag="ISB17_000095"
     CDS             complement(9916..10359)
                     /locus_tag="ISB17_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAP1978086.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10410..10712
                     /gene="yhbQ"
                     /locus_tag="ISB17_000096"
     CDS             10410..10712
                     /gene="yhbQ"
                     /locus_tag="ISB17_000096"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAP1978087.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQTPEIKSD"
     gene            complement(10699..11202)
                     /locus_tag="ISB17_000097"
     CDS             complement(10699..11202)
                     /locus_tag="ISB17_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1978088.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11196..11720)
                     /locus_tag="ISB17_000098"
     CDS             complement(11196..11720)
                     /locus_tag="ISB17_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAP1978089.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11929..12924
                     /locus_tag="ISB17_000099"
     CDS             11929..12924
                     /locus_tag="ISB17_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP1978090.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12933..13811
                     /locus_tag="ISB17_000100"
     CDS             12933..13811
                     /locus_tag="ISB17_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP1978091.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13892..14899
                     /locus_tag="ISB17_000101"
     CDS             13892..14899
                     /locus_tag="ISB17_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAP1978092.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15017..16261)
                     /gene="mtr"
                     /locus_tag="ISB17_000102"
     CDS             complement(15017..16261)
                     /gene="mtr"
                     /locus_tag="ISB17_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAP1978093.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16415..18304)
                     /locus_tag="ISB17_000103"
     CDS             complement(16415..18304)
                     /locus_tag="ISB17_000103"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAP1978094.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18297..18377)
                     /gene="yrbN"
                     /locus_tag="ISB17_000104"
     CDS             complement(18297..18377)
                     /gene="yrbN"
                     /locus_tag="ISB17_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAP1978095.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18484..19368)
                     /gene="nlpI"
                     /locus_tag="ISB17_000105"
     CDS             complement(18484..19368)
                     /gene="nlpI"
                     /locus_tag="ISB17_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAP1978096.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19477..21612)
                     /gene="pnp"
                     /locus_tag="ISB17_000106"
     CDS             complement(19477..21612)
                     /gene="pnp"
                     /locus_tag="ISB17_000106"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP1978097.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21859..22128)
                     /gene="rpsO"
                     /locus_tag="ISB17_000107"
     CDS             complement(21859..22128)
                     /gene="rpsO"
                     /locus_tag="ISB17_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP1978098.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22277..23221)
                     /gene="truB"
                     /locus_tag="ISB17_000108"
     CDS             complement(22277..23221)
                     /gene="truB"
                     /locus_tag="ISB17_000108"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP1978099.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23221..23622)
                     /gene="rbfA"
                     /locus_tag="ISB17_000109"
     CDS             complement(23221..23622)
                     /gene="rbfA"
                     /locus_tag="ISB17_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP1978100.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23786..26458)
                     /gene="infB"
                     /locus_tag="ISB17_000110"
     CDS             complement(23786..26458)
                     /gene="infB"
                     /locus_tag="ISB17_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP1978101.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26483..27970)
                     /gene="nusA"
                     /locus_tag="ISB17_000111"
     CDS             complement(26483..27970)
                     /gene="nusA"
                     /locus_tag="ISB17_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP1978102.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27998..28450)
                     /gene="rimP"
                     /locus_tag="ISB17_000112"
     CDS             complement(27998..28450)
                     /gene="rimP"
                     /locus_tag="ISB17_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP1978103.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28657..28733)
                     /locus_tag="ISB17_000113"
     tRNA            complement(28657..28733)
                     /locus_tag="ISB17_000113"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28697..28699),aa:Met,seq:cat)
     gene            29081..30424
                     /gene="argG"
                     /locus_tag="ISB17_000114"
     CDS             29081..30424
                     /gene="argG"
                     /locus_tag="ISB17_000114"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAP1978104.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30432..32057)
                     /locus_tag="ISB17_000115"
     CDS             complement(30432..32057)
                     /locus_tag="ISB17_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP1978105.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIIFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(32514..32600)
                     /locus_tag="ISB17_000116"
     tRNA            complement(32514..32600)
                     /locus_tag="ISB17_000116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32564..32566),aa:Leu,seq:gag)
     gene            complement(32615..32947)
                     /gene="secG"
                     /locus_tag="ISB17_000117"
     CDS             complement(32615..32947)
                     /gene="secG"
                     /locus_tag="ISB17_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP1978106.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33175..34512)
                     /gene="glmM"
                     /locus_tag="ISB17_000118"
     CDS             complement(33175..34512)
                     /gene="glmM"
                     /locus_tag="ISB17_000118"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP1978107.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34505..35353)
                     /gene="folP"
                     /locus_tag="ISB17_000119"
     CDS             complement(34505..35353)
                     /gene="folP"
                     /locus_tag="ISB17_000119"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAP1978108.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35443..37377)
                     /gene="ftsH"
                     /locus_tag="ISB17_000120"
     CDS             complement(35443..37377)
                     /gene="ftsH"
                     /locus_tag="ISB17_000120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP1978109.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37477..38106)
                     /gene="rlmE"
                     /locus_tag="ISB17_000121"
     CDS             complement(37477..38106)
                     /gene="rlmE"
                     /locus_tag="ISB17_000121"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAP1978110.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38232..38525
                     /gene="yhbY"
                     /locus_tag="ISB17_000122"
     CDS             38232..38525
                     /gene="yhbY"
                     /locus_tag="ISB17_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP1978111.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38681..39157)
                     /gene="greA"
                     /locus_tag="ISB17_000123"
     CDS             complement(38681..39157)
                     /gene="greA"
                     /locus_tag="ISB17_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP1978112.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39405..40838
                     /gene="dacB"
                     /locus_tag="ISB17_000124"
     CDS             39405..40838
                     /gene="dacB"
                     /locus_tag="ISB17_000124"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP1978113.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40879..42051)
                     /gene="cgtA"
                     /locus_tag="ISB17_000125"
     CDS             complement(40879..42051)
                     /gene="cgtA"
                     /locus_tag="ISB17_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAP1978114.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42067..43032)
                     /locus_tag="ISB17_000126"
     CDS             complement(42067..43032)
                     /locus_tag="ISB17_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1978115.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43159..43416)
                     /gene="rpmA"
                     /locus_tag="ISB17_000127"
     CDS             complement(43159..43416)
                     /gene="rpmA"
                     /locus_tag="ISB17_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP1978116.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43437..43748)
                     /gene="rplU"
                     /locus_tag="ISB17_000128"
     CDS             complement(43437..43748)
                     /gene="rplU"
                     /locus_tag="ISB17_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP1978117.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44007..44978
                     /gene="ispB"
                     /locus_tag="ISB17_000129"
     CDS             44007..44978
                     /gene="ispB"
                     /locus_tag="ISB17_000129"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAP1978118.1"
                     /translation="MNLEKINELTAQDMAGVNASILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45211..45489
                     /gene="sfsB"
                     /locus_tag="ISB17_000130"
     CDS             45211..45489
                     /gene="sfsB"
                     /locus_tag="ISB17_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAP1978119.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45537..46796)
                     /gene="murA"
                     /locus_tag="ISB17_000131"
     CDS             complement(45537..46796)
                     /gene="murA"
                     /locus_tag="ISB17_000131"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP1978120.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46851..47105)
                     /gene="ibaG"
                     /locus_tag="ISB17_000132"
     CDS             complement(46851..47105)
                     /gene="ibaG"
                     /locus_tag="ISB17_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAP1978121.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47265..47558)
                     /gene="mlaB"
                     /locus_tag="ISB17_000133"
     CDS             complement(47265..47558)
                     /gene="mlaB"
                     /locus_tag="ISB17_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAP1978122.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47558..48193)
                     /gene="mlaC"
                     /locus_tag="ISB17_000134"
     CDS             complement(47558..48193)
                     /gene="mlaC"
                     /locus_tag="ISB17_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAP1978123.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48212..48763)
                     /gene="mlaD"
                     /locus_tag="ISB17_000135"
     CDS             complement(48212..48763)
                     /gene="mlaD"
                     /locus_tag="ISB17_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAP1978124.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48768..49550)
                     /gene="mlaE"
                     /locus_tag="ISB17_000136"
     CDS             complement(48768..49550)
                     /gene="mlaE"
                     /locus_tag="ISB17_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAP1978125.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49558..50367)
                     /gene="mlaF"
                     /locus_tag="ISB17_000137"
     CDS             complement(49558..50367)
                     /gene="mlaF"
                     /locus_tag="ISB17_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAP1978126.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50577..51554
                     /locus_tag="ISB17_000138"
     CDS             50577..51554
                     /locus_tag="ISB17_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAP1978127.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51568..52554
                     /gene="kdsD"
                     /locus_tag="ISB17_000139"
     CDS             51568..52554
                     /gene="kdsD"
                     /locus_tag="ISB17_000139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAP1978128.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52575..53141
                     /gene="kdsC"
                     /locus_tag="ISB17_000140"
     CDS             52575..53141
                     /gene="kdsC"
                     /locus_tag="ISB17_000140"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAP1978129.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53138..53713
                     /gene="lptC"
                     /locus_tag="ISB17_000141"
     CDS             53138..53713
                     /gene="lptC"
                     /locus_tag="ISB17_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAP1978130.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53682..54239
                     /gene="lptA"
                     /locus_tag="ISB17_000142"
     CDS             53682..54239
                     /gene="lptA"
                     /locus_tag="ISB17_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAP1978131.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54246..54971
                     /gene="lptB"
                     /locus_tag="ISB17_000143"
     CDS             54246..54971
                     /gene="lptB"
                     /locus_tag="ISB17_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAP1978132.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55019..56452
                     /gene="rpoN"
                     /locus_tag="ISB17_000144"
     CDS             55019..56452
                     /gene="rpoN"
                     /locus_tag="ISB17_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP1978133.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56475..56762
                     /gene="hpf"
                     /locus_tag="ISB17_000145"
     CDS             56475..56762
                     /gene="hpf"
                     /locus_tag="ISB17_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAP1978134.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56880..57371
                     /gene="ptsN"
                     /locus_tag="ISB17_000146"
     CDS             56880..57371
                     /gene="ptsN"
                     /locus_tag="ISB17_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAP1978135.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57417..58271
                     /gene="rapZ"
                     /locus_tag="ISB17_000147"
     CDS             57417..58271
                     /gene="rapZ"
                     /locus_tag="ISB17_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP1978136.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58268..58540
                     /gene="npr"
                     /locus_tag="ISB17_000148"
     CDS             58268..58540
                     /gene="npr"
                     /locus_tag="ISB17_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAP1978137.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58753..59385
                     /gene="yrbL"
                     /locus_tag="ISB17_000149"
     CDS             58753..59385
                     /gene="yrbL"
                     /locus_tag="ISB17_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAP1978138.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59382..60110)
                     /gene="mtgA"
                     /locus_tag="ISB17_000150"
     CDS             complement(59382..60110)
                     /gene="mtgA"
                     /locus_tag="ISB17_000150"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAP1978139.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(60107..60760)
                     /gene="elbB"
                     /locus_tag="ISB17_000151"
     CDS             complement(60107..60760)
                     /gene="elbB"
                     /locus_tag="ISB17_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAP1978140.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60990..63326)
                     /gene="arcB"
                     /locus_tag="ISB17_000152"
     CDS             complement(60990..63326)
                     /gene="arcB"
                     /locus_tag="ISB17_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAP1978141.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63422..64351)
                     /locus_tag="ISB17_000153"
     CDS             complement(63422..64351)
                     /locus_tag="ISB17_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP1978142.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            65026..69486
                     /gene="gltB"
                     /locus_tag="ISB17_000154"
     CDS             65026..69486
                     /gene="gltB"
                     /locus_tag="ISB17_000154"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAP1978143.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69499..70917
                     /locus_tag="ISB17_000155"
     CDS             69499..70917
                     /locus_tag="ISB17_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HAP1978144.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(70985..71449)
                     /locus_tag="ISB17_000156"
     CDS             complement(70985..71449)
                     /locus_tag="ISB17_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1978145.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(71446..72321)
                     /locus_tag="ISB17_000157"
     CDS             complement(71446..72321)
                     /locus_tag="ISB17_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAP1978146.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(72318..73007)
                     /locus_tag="ISB17_000158"
     CDS             complement(72318..73007)
                     /locus_tag="ISB17_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP1978147.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(73055..74545)
                     /gene="nanT"
                     /locus_tag="ISB17_000159"
     CDS             complement(73055..74545)
                     /gene="nanT"
                     /locus_tag="ISB17_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAP1978148.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(74654..75547)
                     /gene="nanA"
                     /locus_tag="ISB17_000160"
     CDS             complement(74654..75547)
                     /gene="nanA"
                     /locus_tag="ISB17_000160"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP1978149.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(75669..76460)
                     /gene="nanR"
                     /locus_tag="ISB17_000161"
     CDS             complement(75669..76460)
                     /gene="nanR"
                     /locus_tag="ISB17_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAP1978150.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(76568..77065)
                     /gene="sspB"
                     /locus_tag="ISB17_000162"
     CDS             complement(76568..77065)
                     /gene="sspB"
                     /locus_tag="ISB17_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAP1978151.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77071..77709)
                     /gene="sspA"
                     /locus_tag="ISB17_000163"
     CDS             complement(77071..77709)
                     /gene="sspA"
                     /locus_tag="ISB17_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAP1978152.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78104..78496)
                     /gene="rpsI"
                     /locus_tag="ISB17_000164"
     CDS             complement(78104..78496)
                     /gene="rpsI"
                     /locus_tag="ISB17_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP1978153.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78512..78940)
                     /gene="rplM"
                     /locus_tag="ISB17_000165"
     CDS             complement(78512..78940)
                     /gene="rplM"
                     /locus_tag="ISB17_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP1978154.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79160..80287)
                     /locus_tag="ISB17_000166"
     CDS             complement(79160..80287)
                     /locus_tag="ISB17_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAP1978155.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80481..80879
                     /locus_tag="ISB17_000167"
     CDS             80481..80879
                     /locus_tag="ISB17_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAP1978156.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81033..82400
                     /gene="degQ"
                     /locus_tag="ISB17_000168"
     CDS             81033..82400
                     /gene="degQ"
                     /locus_tag="ISB17_000168"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAP1978157.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            82490..83557
                     /gene="degS"
                     /locus_tag="ISB17_000169"
     CDS             82490..83557
                     /gene="degS"
                     /locus_tag="ISB17_000169"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAP1978158.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83620..84558)
                     /gene="mdh"
                     /locus_tag="ISB17_000170"
     CDS             complement(83620..84558)
                     /gene="mdh"
                     /locus_tag="ISB17_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAP1978159.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            84993..85463
                     /gene="argR"
                     /locus_tag="ISB17_000171"
     CDS             84993..85463
                     /gene="argR"
                     /locus_tag="ISB17_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAP1978160.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        20976 a        23113 c        21304 g        20182 t
ORIGIN      
        1 tgagcagtcc aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg
       61 gcgtgacctg gacatccacc atcggtgcga atctgttggt agtcgtggat gatgttgtcg
      121 ctaacgatga tattcaacag aaattaatgg gtattaccgc agaaacctac ggctttggca
      181 ttcgtttctt tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccacatcaga
      241 agatcttcct gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggtattg
      301 acctgaaaga tgtcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca
      361 gtaaagttta tgtcgatgac caggatctca cggacttacg ttatattaaa caacgtggcg
      421 tgaatgtttt cattcaggac gttcctggcg atcaaaaaga acaaatccct gactaaatct
      481 gaaatcgcct taatattggt ttgaggtaat aaaaatgcat gaaataaccc tactccaggg
      541 attatccctg gcggcgttag ttttcgttct ggggattgat ttttggctgg aagccttatt
      601 tttattccgc ccgataatcg tttgtaccct tacaggtgct attctcggtg atattcagac
      661 tggcttaatt accggaggcc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg
      721 tgttcagccg cccaacccta ttatggcggg tctgatgacc accgtcattg catggtctac
      781 gggcgttgat gccaaaacgg caattggtct tggcctgccg tttagtttgt taatgcagta
      841 cgtcattctg ttcttctatt ccgctttctc attatttatg accaaagccg ataaatgcgc
      901 gaaagaggcg gatacggcag cgttttcccg acttaactgg acaacgatgc tcatcgtcgc
      961 ttcagcgtat gcggtgattg ctttcctctg tacttacctg gcacaagggg cgatgcaggc
     1021 gctggtgaaa gcgatgcccg cctggctgac ccacggcttt gaagtggctg gcggtattct
     1081 gcctgccgtt ggttttggct tgctgctgcg cgtgatgttc aaagcgcaat atatccctta
     1141 cctgatcgcc ggtttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc
     1201 cgtgctgggc gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc
     1261 gcaaccgcag cccgttgcca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa
     1321 tcagtaaaaa agatatcacc cgtctgggct ttcgttcgtc actgctacaa gcaagcttta
     1381 actacgaaag gatgcaggcg ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga
     1441 tttataagaa cgacaaaccg ggcctaagcg cggcgatgaa agataacctc gaatttatta
     1501 atacccaccc taatctggtc ggatttctga tggggttatt aatttcgatg gaagaaaaag
     1561 gagaaaaccg cgacaccatt aaaggcctca aagtggcact gtttggccca atcgccggga
     1621 ttggcgatgc gattttctgg tttactttgt tgccgattat ggcggggatt tgctcatcgt
     1681 ttgccagtca ggggaacctg ttgggaccga ttttgttttt cgccgtttac ctgcttatct
     1741 ttttcctgcg cgtcggctgg acccacgtcg gttattcagt cggcgtgaag gctattgata
     1801 aagtgcgaga gaactcgcag atgattgccc gttcggcaac catcctcggg atcacagtaa
     1861 tcggcgggct gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca
     1921 gtacccacag cgtcgcgctg cagcaggatt tcttcgataa agtcttcccg aatattttac
     1981 cgatggccta caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc
     2041 tgttaatcgg cgtgacgttt gttctttcta ttgtttgttc tgcattcggc attttgtaaa
     2101 tggaacgagg caccgcgtct ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa
     2161 cccttcaaca agttgaaaac tatacggcgt taagtgaacg tgccagcgaa tatttattgg
     2221 ccgtgatccg tagcaaaccg gatgccgtga tttgcctggc gaccggagcc acgccattac
     2281 tgacgtatca ttatttggta gaaaaaatcc accagcagca ggttgatgtc agccagctca
     2341 ccttcgtgaa gctcgacgaa tgggtggatc tgccattaac gatgcctggt acctgcgaaa
     2401 ctttccttca acagcatatc gtgcagccgc tggggctacg tgaagaccag ctcatcagct
     2461 ttcactccga agagataaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca
     2521 aaggcggtct ggacttatgc gttctcggat tggggaaaaa cggtcatctt gggctgaacg
     2581 aaccgggaga aagcctgcaa ccggcctgcc atatcagtca acttgatgcc agaacacagc
     2641 aacatgagat gttaaaaacc gctgaccgtc ccgtgactcg tgggatcacc ttaggcctga
     2701 aggatattct caatgcccgc gaagttttgt tactgataac tggcgaagga aaacaggatg
     2761 cgacagaacg ttttctcacg gctaaagtct ccaccgctat cccggcttca tttttatggc
     2821 tgcataacca tttcacatgc gtgattgatg aaatgtgtcg tcgttaactc gcctggtaca
     2881 ggcgttgcag cgctattcct gctgcgacgc ctgtaatcac tctccctgct gctccagcgc
     2941 atacttatac agcgcatttt tcttcacgcc gtgaatttct gcggccagcg ccgccgcttt
     3001 tttcagcggc agttctgcct gtaacagcgc cagcgtgcgc agggcatcag caggcaggtc
     3061 atcttcctgc gctttatggc cttcgacaat cagcaccatt tcgcctttgc gacggttttc
     3121 atcttctttt acccacgcca gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt
     3181 tttggttagc tcacgcgcca gaaccacgta acgggattcg cctaataccg caacaatatc
     3241 ttccaggcta tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc
     3301 ttcaatagct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcata
     3361 acaaaaacga tcggatggta aacccgcggc gcttaacgca gtgatagcag cacacggccc
     3421 tggtagcggc accacgcgaa tccccgcttc acgacaggta cgcaccagat ggtagccagg
     3481 atcgttaatt agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag
     3541 cttcgccagc agcgtttcgg ctttttgttg ttcgttatga tcgtgcagcg caaacaaccg
     3601 ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgcgtatcct cggcggcaat
     3661 cagatcaacg gcctgtaata cctctaatgc acgctgggtg atatccgcca gattgccgat
     3721 tggcgtcggt acaatgtaaa gctgcccctg agaattatcc gccgattggt gttgtttcat
     3781 tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaaata tcactggata
     3841 cattatggta ccctcaacat tttctcgttt gaaagccgcg cgttgtctgc ctgttgtttt
     3901 ggcagccctg attttcgccg gttgtggcac ccatactccc gatcagtcca ctgcttatat
     3961 gcagggcacg gcgcaggctg attctgcttt ttacctgcaa cagatgcagc aaagttctga
     4021 tgataccagg atcaactggc aattactcgc cattcgtgca ctggtgaaag aaggtaaaac
     4081 cgggcaggcg gttgagttgg ttaaccaact accgcaagaa ctgaatgata atcagcgtcg
     4141 cgagaaaaca ctgctggcgg cagagattaa actggcgcag aaagattttg ctggcgcgca
     4201 aaacttgctg gcgaaaatca cacctgccga tttagaacaa aaccagcaag cccgttactg
     4261 gcaggcaaaa atcgatgcca gccaggggcg tccttccatt gatttactgc gcgcgttaat
     4321 tgctcaggaa ccgctgctcg gtgcgaaaga aaagcagcag aatattgacg ccacatggca
     4381 ggcgctctcc tccatgactc aggagcaggc gaatacgctg gtgatcaacg ccgacgaaaa
     4441 tattctgcaa ggctggctgg atttgcagcg cgtctggttt gataaccgta acgatcctga
     4501 catgatgaaa gctgggatcg ccgactggca gaaacgttat ccgaacaacc cgggcgcgaa
     4561 aatgctgcca acgcagttgg ttaacgtaaa agcgtttaaa ccagcatcga ccaacaaaat
     4621 cgccctgctg ttgccgctga atggccaggc agcggtattt ggtcgcacta ttcagcaagg
     4681 ctttgaagcg gcgaaaaata tcggcactca gccagtggcg gcccaggtag ctgccgcacc
     4741 tgccgcagac gtagcagaac aacctcagcc gcaaaccgtg gatggcgttg ccagcccggc
     4801 acaagcttcg gttagcgatc tgaccggtga acagcctgcg gcccagccgg tacctgtaag
     4861 cgccccggcg acaagcaccg cagcggtaaa cgcacccgca aatccatccg cagagctgaa
     4921 aatctacgat acctcatcac aaccacttag ccagatctta agccaggttc agcaggatgg
     4981 tgcgagtatt gtggtcggtc cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa
     5041 caccccgctg aacgtgctgg cactgaacca gccggagaat atcgaaaacc gcgtcaatat
     5101 ttgttacttc gcgctttcgc cagaagacga agcgcgcgat gcagcgcgtc atattcgtga
     5161 ccagggtaaa caagcgccgc tggtgctgat cccgcgcagt tcattgggcg atcgcgtagc
     5221 caatgcgttt gcgcaagagt ggcagaaact gggtggcggc accgttctgc aacaaaaatt
     5281 tggttccacc agcgaattac gcgcgggtgt taacggcggt tctggtattg ctttaacggg
     5341 tagcccgatt actcccagag cgacaaccga ctccggcatg acgaccaaca atccaacgct
     5401 gcaaaccacg ccaaccgatg accagttcac caataatggc ggtcgtgtcg atgcggtgta
     5461 cattgtggca acgccgggtg aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg
     5521 tagccagagc ggtgcaacgc tgtacgccag ttcccgcagt gcgcaaggga ccgctggccc
     5581 ggatttccgc ctggagatgg aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg
     5641 taatctgccg ttaatgcagc aggcgctcag cgcggtgaac aacgattatt cgctggctcg
     5701 catgtatgcg atgggtgtcg atgcctggtc gttggcaaat catttctcac aaatgcgcca
     5761 ggttcagggt tttgaaatca acggtaatac cggaagcctg acggcaagcc cggattgcgt
     5821 gattaacagg aagttatcat ggctacagta ccaacaaggt caggtagtcc ccgccagtta
     5881 accaccaaac agaccggcga tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa
     5941 ggactacggt ttatcgccgc taacgtgaac gagcgtggcg gcgagatcga tctgataatg
     6001 cgtgaaggcc ggaccaccgt ttttatcgag gtgcgttacc gccgctctgc gctttatggc
     6061 ggcgcggcag ccagtgtgac ccgcagcaaa caacacaaat tattacagac tgcccgtttg
     6121 tggctcgcgc gtcataatgg gagttttgat actgtggatt gccggttcga tgtggtagcc
     6181 ttcaccggga atgaggttga gtggattaag gatgccttta atgaccactc ataattaagg
     6241 tttaaggatt agcgtgcaag aaagaattaa agcttgcttc actgaaagca ttcaaactca
     6301 aattgcggcg gcagaggcgc ttccggatgc catctcccgt gcagccatga cgctggttca
     6361 gtctctgctc aatggcaaca aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc
     6421 acagcatttt gctgccagca tgatcaaccg tttcgaaacg gagcggccca gcttacctgc
     6481 cattgcacta aatactgata atgttgtctt aacggcaatt gccaacgatc gcttacatga
     6541 tgaagtgtat gcaaaacagg tgcgggcgct gggtcatgcg ggagatgtat tgttagccat
     6601 ttccacccgt ggcaacagcc gcgatattgt aaaagcggta gaagctgccg ttacgcgtga
     6661 tatgaccatt gtggcattaa ccggctatga cggcggtgaa cttgcaggtt tgttagggcc
     6721 acaggatgtg gagatccgca ttccttcgca tcgtagtgct cgcattcagg aaatgcatat
     6781 gctgacggta aattgcctgt gcgatctgat cgataacacg cttttccctc accaggatgt
     6841 ttaaggagaa tacatgaagg cattatcgcc aatcgcagtc cttatttccg cgctgctgtt
     6901 gcaaggttgt gttgccgctg ccgtagtggg tactgctgct gtgggtacca aagccgcaac
     6961 tgacccacgc agtgtcggca cccaggtaga cgatggtacc ttggaagtgc gcgtgaacag
     7021 cgcattgtcg aaagacgaac aaattaagaa agaagcgcgc attaatgtaa cggcctatca
     7081 gggcaaagtg ctgctggttg ggcagtcacc aaacgctgaa ctttcggctc gcgccaaaca
     7141 gattgctatg ggcgtagacg gtgccaacga agtgtataac gagattcgtc agggccagcc
     7201 gattggtctg ggcgaagcgt ctaacgatac gtggatcacc accaaagtac gttcgcagct
     7261 gttaaccagc gacctggtga aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt
     7321 gttcctgatg gggctggtga ctgaacgtga agcgaaagcg gcggcagata ttgccagccg
     7381 ggtaagcggc gtgaagcggg taactacggc gtttacgttt attaaatagc agcccttgta
     7441 atgcctgatg cgacgcttgc agcgtcttat caggcctaca acgatgcaaa ccgtaggtcg
     7501 gataaggcgt ttacgccgca tccgacatgt tttccctcaa atcaaactaa cagcgccagc
     7561 ccgccgacaa tcacaccaga cactgccacc atcgcccccg tcagccataa ggctttcgcc
     7621 gggaacgctt tgcgcagcat aatcagtgac ggcaaactca ccgccgggag cgtcatcaac
     7681 aatgccagcg ccggagcagt tcccatacct gccagcatca tcgtttgtac aatcggaatt
     7741 tctgctgccg tgggaatgac aaacaagcat cctgccaccg ccatcgccac cacccacatc
     7801 aggctgttat cgacagcacc atcggcatgg gggaataacc agacgcgagc ggcacccaac
     7861 accagtactg caaggatgta aaccgggatc gtactccaga aaagcgtcca tagcgccctg
     7921 ccccagcggc taaaaaatcc tccctgtgct tccggtatat caatttcgac cggtgcctgc
     7981 gtttgcggtg tttcacgcac ccatttttgc accagcgtcg caatcagcaa caccatcacc
     8041 agccccgcca ccagacgaat cgccgcaaaa ccccagccga ggacgaaccc cataaacacc
     8101 agcgtcgccg ggtttaacaa cggattcccc atccagaatg ccagcgcacc gcccatcgac
     8161 acctgttggc gacgcattcc cgccgcgacc ggagccgcac agcaggtaca catcataccc
     8221 ggcagcgaaa acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc
     8281 aacaaccagt cacgcgggat cagtacctga atcaacgaac cgaggatcac ccccagcacc
     8341 gccgctttcc ataccgcgag gaaatagatc atcgcgtaat ccaacgccgc ctgccacgga
     8401 ttagcatccg cctgcgcaag gatagattta ccgatactgt gggtttcggc agcagtaaac
     8461 gctttgccgt agtaaggttg ccatttcaca taccagagac cggcaatgac aacgagaaag
     8521 aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc
     8581 atagcattcc ccaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca
     8641 attcttggga ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcaca
     8701 ccttcatgct caggccgcat cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca
     8761 atggatttcc agttaccggg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc
     8821 cgctgtttgc tacgatcacc cagtaacatc gacgggcgag caatggtcaa tttcggccag
     8881 ttctgggcga ttaatgcttc ttccatctcc cctttgacgc ggttatagaa aaacggcgag
     8941 tgggcattag cacccatagc actgaccacc agcatatgct gcgcaccgag ccgccgcccg
     9001 gttaatgcgg tatccaccac caacgtgtaa tcggcatgaa taaacgcctc tttgctcccc
     9061 gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt cgatgggatc ggtgacctgc
     9121 gccagcgcgt cagtcagttg cggatcatgg ggattaaaca ccccgggcat atcgcccaac
     9181 ggacgtcgcg tcggcgcagc aatgacgtta actttcggtt cattaatcaa catccgcagc
     9241 aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta cctgactcat cttcgctcct
     9301 ttacagaatt gtctgccttg cgctccccag ctcatgcacc aggcttaata gaccatgagg
     9361 taattatccc cgattgtgga aaattcgcct catccaatgc aacaacgcgg aggaagcatg
     9421 agtaagaaaa tcgccgcttt aatcactgat gaatttgagg attcagaatt cacttcaccc
     9481 gcagacgagt tccgtaaagc cggacacgaa gtgattacca ttgaaaaaca agcgggtaaa
     9541 acggtgaaag gcaaaaaagg agaagccagc gtggtcatcg ataaatccat cgatgaagtg
     9601 acgcccgcag agttcgatgc cctgctgcta ccgggcggtc attcaccaga ttatctgcgt
     9661 ggcgacaacc gttttgtcac cttcactcgc gattttgtga acagcggcaa acctgttttc
     9721 gccatctgtc acggtcccca gttgctgatc agcgccgatg tgatccgtgg tcgtaagctg
     9781 accgccgtta aaccgatcgt gattgatgtt aaaaacgcgg gcgcggaatt ttacgatcag
     9841 gaagtggtgg tcgataaaga tcagctggtt accagccgga caccggacga tctgccagcg
     9901 tttaaccgcg aggcgttacg cctgctcggt gcctgagtta cgtagccaaa tcattttttt
     9961 accaaagccc agcgtgttgt cggtgaattt gatttcatcg agccggattt cccataccgg
    10021 ggccgacagc attctggcaa ccggaaagcg acgattgtac gccttgcgtg cgaggtcgct
    10081 ttcctcgcct tccagtctgc ggatctcacc tttaaactgc acgccgcgaa ttaacgctac
    10141 cgttttcggc tgaccgttta ccgttccggc aattgccgcc tgcggcccgc tcatctgcgc
    10201 atgacgcgtt ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat caaaaagata
    10261 aaaggcattg gcgcaccaca gttccccttc ctgctgcaca caccaggtga caacatgttg
    10321 ttttgccagc caacggctga tggcggtgag tgtttccatt gctgttctct tttatactgt
    10381 gggcctgacc ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta
    10441 ccgccgacaa taagctttat accgggatca ccacggatgt cgaacgccgc tatcagcagc
    10501 accaaagcgg caaaggggcg aaagcactgc gagggaaagg agaactaacg ctggcgtttt
    10561 ccgcgccagt gggcgaccgt tcgctggcac tacgagcgga atatcgcgtt aaacaactga
    10621 ctaaacggca aaaagagcgt ctggtaacgg aaggcgcagg gtttgcagaa ctgttgagca
    10681 gcctgcaaac cccggagatt aaaagcgatt gaaatgctcg tgatactcga ccagaccagt
    10741 tacgccgttc agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg
    10801 ccagcggcaa tgcagatcgt gatgcgccgc cagttcaaag ccgaaacggc tgtacagcgc
    10861 cggatcgccc agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc
    10921 atagaccagt tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccagcggtgc
    10981 catgccgacc cattgcaggt cttcgccctg cacatcaacc ggactaaaag ccacatagcc
    11041 aatgacctga ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg
    11101 cagatcgtga accagcttcg cttccgcatc actttcgaat gaacgacgca gcagggcatc
    11161 aatacccggc gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc
    11221 tgtttggttt caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc
    11281 atcatgcgta aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca
    11341 tacagcccca gctccgtatc gccttcaatc accagccgac gctggaagaa gagcgtatcc
    11401 ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca
    11461 tcagcttgcg cgttctggct aacgaccagt ttgccattca ccaccgaggt aaaccattgc
    11521 aggtcaatat cacgcacatg aatacttaac cagcgacctt caagaaactc cagctcgcca
    11581 tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc
    11641 gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg
    11701 gaacgcagtt tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca
    11761 tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac
    11821 caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca
    11881 gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc
    11941 tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt
    12001 tatatcgggc taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag
    12061 aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg
    12121 attaacactt ttgcgcatcc ggacggttac gcccgttggc agcacgccgt ggatatggcg
    12181 gcgcaactgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc
    12241 gaacgttatc cgcatattga gcgccacgta tcggtgcagg cttcggcgac caatgaagag
    12301 gcgattaact tttatcatcg ccattttgac gttgcccgcg tggtactgcc gcgcgtgttg
    12361 tcgattcatc aggtgaaaca gctggcacgg gtcacacctg taccactgga agtgtttgct
    12421 ttcggcagcc tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt
    12481 gaatcgccca acaccgtggg ggcatgttct ccggcccgtt tcgtgcgctg gcagcaaacg
    12541 ccgcaagggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa
    12601 aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac
    12661 gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg
    12721 aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg
    12781 gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg
    12841 caaagcgcgt ggatggagac gctcgggtcg atgtccgaag gcacgcaaac tacccttggc
    12901 gcgtatcacc gtaaatggca gtgagaaaag caatgaaata ttccttaggg ccggtgctat
    12961 ggtactggcc aaaagagacg ctggaagact tttatcagca ggccgccgcc agcagcgcag
    13021 acgtgattta tcttggtgaa gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact
    13081 ggctggagat ggcaaaatca ctcgccggaa gtggtaagca gattgtgctc tccacgctgg
    13141 cgctggtgca ggcatcctct gaactgggcg aactgaaacg ctatgttgag aacggtgagt
    13201 ttctgattga agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt
    13261 tcgtcgccgg gcacgcgctg aactgctaca acgcggtgac gctgaaaata ttgcttaaac
    13321 agggcatgat gcgctggtgt atgccggtgg agctttcccg cgactggctg gtgaatctgc
    13381 ttaatcagtg cgatgagttg ggcattcgca accagtttga agtggaagtc ctgagctacg
    13441 gtcatctacc gctggcctac tccgcacgct gctttaccgc gcgttcggaa gaccgcccga
    13501 aagatgagtg tgaaacctgc tgcattaagt atccgaacgg gcgcaacgta ctgtcgcagg
    13561 aaaaccaaca agtgtttgta ctcaacggca ttcagaccat gagcggctac gtttacaacc
    13621 tcggtaacga gctggcatcc atgcaggggc tggtcgatgt ggtccgcctg tcaccgcagg
    13681 gtactgacac tttcgcaatg ctcgatgcct tccgcgctaa tgaaaatggc gctgcgccac
    13741 taccgttgac ggcaaacagc gactgtaacg gctactggcg gcggctggcc gggctggagc
    13801 tgcaagcgta agtatatagc tcactttgtt aacaacttta actactcttt aatgcagtat
    13861 taaagactaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct
    13921 actcgatctg gcccccatcc ccgaaggttc ttcagcgcgg gaagcgttct cccactctct
    13981 cgacctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca
    14041 caatatgacc ggcattgcca gtgctgcaac gtcggtgtta attggctatc tggcggcgaa
    14101 taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt
    14161 cattgccgaa cagttcggca cgcttaatac gctctatccg gggcgaatcg atttggggct
    14221 gggtcgcgcg ccgggtagcg accaacggac aatgatggcg ctacgtcgtc atatgagcgg
    14281 cgatattgat aatttccctc gcgatgtcgc ggaactggtg gactggtttg atgcccgcga
    14341 tcccaatccg catgtgcgcc ctgtaccggg ctatggcgag aaaatcccgg tgtggttgct
    14401 aggctccagc ctttacagtg cacaactggc ggcgcagctt ggtctgccgt tcgcgtttgc
    14461 ctcacacttc gcgccggata tgctgttcca ggcgctgcat ctttatcgca gcaacttcaa
    14521 accgtcggca cggctggaga aaccgtatgc gatggtgtgc atcaatatta ttgccgccga
    14581 cagtaaccgc gacgctgagt ttctgtttac ctcaatgcag caagcctttg tgaagctgcg
    14641 tcgcggcgaa accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcacc
    14701 gtccgaacag tatggcgtgc agcaggcgct aagtatgtcg ctggtgggcg ataaagcgaa
    14761 agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggttaa
    14821 cgggcagatt ttcgaccacc aggcacgcct gcattcgttt gagctggcga tggatgtgaa
    14881 ggaagagttg ttggggtagt gtgttttaac gtaggaagcc ttatccgaga tggcaacgct
    14941 gtcctacgta ggcctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg
    15001 gataaggcac ctctgattac tgatacaccg gcagcacatt aaagctcgat aagatatgca
    15061 ccagtgcgtt gccgacgcca aacaccagaa tcagcacaat catcggcttg ccgccccata
    15121 cacggaattt cgggctacca aagcgtttac gcgatgcgcg ggccagcagc gccggaacaa
    15181 ttgccgccca gatggtagcc gctaagccag cataaccaat ggcgtacagg aatccgttag
    15241 gaaacagcag gccacccaca accggcgggg caaaggtcag caacgctgtt ttcaaacggc
    15301 ccacagccga gtcgtcgaaa ccaaacagat ctgccagata gtcaaacagg cccagcgtta
    15361 cgccaaggaa cgaactcgct accgcaaagt ttgagaacac aaccagcaga aggtccaggc
    15421 tacggctgtt cagtacgccg cttaacgcct gtaccagcac atcaatatta ccgcccttct
    15481 cggcgatgcc gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg
    15541 tatacagcgc cagcgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat
    15601 ctttgccgta atacttcatc aggctcggta cgttaccgtg ataaccaaac gatgccagac
    15661 agaacggcag cgtcatcaac agatacggtg cataagacgc attgctttcg gcgacgttga
    15721 acaatgtcgc aggctgcaca tgccccagca ggctaccgaa ggtgaggaag aaggtaatga
    15781 ctttcgcccc cagcacaatc gctgtcatgc gactgacggc tttggtgctc aaccacacca
    15841 caaacgccac caacagtgca aaaccaaaac ccgctgcccg tgccgggacg tttagcgaca
    15901 tctctgcgaa ggtgtgatgc agaatcgaac cactggcaga aatataggca taggtcagga
    15961 tgtagagcac aaaggcaatg gaaatgccgt tgaccacgtt ccagcctttg ccgagcaggt
    16021 ctttggtgat ggtgtcaaag ctcgaaccga ttcggtaatt caggttagct tccagaatca
    16081 tcaagccgga atgcagcata cagaaccagg tgaaaatcag cgccgccatt gaccagaaga
    16141 accacgcccc ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc
    16201 cgataatcac cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca
    16261 tgagggcttc tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca
    16321 gtacatgagt acaaaaaaca gaaaaaaagc cccgatgggt aaaatcgggg ctgtatatat
    16381 tattttacag attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac
    16441 gaccggtaga gtcatcacga cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac
    16501 gcccatcacc acggccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac
    16561 gttcgccacc gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat
    16621 cgcccagtaa ctgcatgttc attggcttgt tgagaatgcg agtacgcgta aagtgttgca
    16681 gcacttcacc cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga
    16741 tgttaccgat gtaacggctg ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac
    16801 gaacttcaac accatcatcg cggcccactt caatacggta caactgcata tcgccaacat
    16861 cacgacgttc gcgacgcgga cgatcttcac ggtcgccacg cgggccacgg tcgttacgat
    16921 cgcgcggacc acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg
    16981 gcggaacaat cagagtacgt tcaccctgtg ccattttcag tagtgcggct gccagagttt
    17041 cgagatccag ctcttcacct tcggcagtcg gctgaatttt gctcagcagt gcacggtatt
    17101 gatccagatc gctgctttcc agctgctgct gtactttagc ggcaaatttt tccagacgac
    17161 gtttgcccag cagttctgcg ttcggcagtt ctacttccgg aatggtcagc ttcatggtgc
    17221 gttcgatgtt gcgcagcaga cgacgctcac ggttctcaac gaacagcagt gcacgaccag
    17281 cacgacctgc acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga
    17341 tatcgtagtt aaccaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg
    17401 tcgcaatcag gatgtccaga cgaccatctt tcaggcgttc cagtgtctgc tcacgcagcg
    17461 cctggttcat gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttctg
    17521 ccacttccag agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg
    17581 cttccaggaa acgtaccagt gcttcgtttt tacgcatacc ccagacagtc cagtagctct
    17641 ggctgatgtc aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca
    17701 taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct
    17761 gatgaccttc cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca
    17821 gcatttcgtc agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac
    17881 gtttcaggtg gtccagcaga cggcccggtg taccgacaac gatctgcggc ccctgacgca
    17941 gggcgcgtaa ttgcacgtca taacgttggc cgccgtacag ggcaaccacg tttacgccgc
    18001 gcatgtgttt agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg
    18061 gtgccagcac cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta
    18121 aagagaatgc tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat
    18181 tcagcagatg tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt
    18241 taagggcttc aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag
    18301 ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat
    18361 tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa
    18421 caacgccctc acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt
    18481 cagctattgc tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc
    18541 caacaatgcg tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa
    18601 cagtgccgtg gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt
    18661 ggtttcactg agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc
    18721 cattaacgtt tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg
    18781 ttccttatcc gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag
    18841 cttctgctcg gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata
    18901 aaacgccagc agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc
    18961 gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc
    19021 ataggcagca tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc
    19081 aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag
    19141 accgagacta tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt
    19201 taaagcccga ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt
    19261 cggttgcaat ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc
    19321 aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg
    19381 aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta
    19441 aaagcccccc gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg
    19501 agcttccggt gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat
    19561 acggccctgg cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta
    19621 atcggtcact ttctcaacgc gtttatcagc gatttgagag atgtggacca gaccttcttt
    19681 accgccgccg atggcaacaa atgcgccaaa gtcaacgatt cgggtcactt taccattgta
    19741 gacgcggcct acttcgattt ctgcagtgat ctcttcgata cgacgaatag cgtttttcgc
    19801 cttctcgccg tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtagt
    19861 gccagtttct tcggtcagag cacggattac agaaccgcct ttaccgataa cgtctttgat
    19921 cttgtctgga ttgatcttga tggtatggat acgcggagcg aactcagaga tatcaccgcg
    19981 cggcgcattg atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg
    20041 gttcagtgca acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa
    20101 tgcagagata ccgtcacgag aacccgcaac tttgaagtcc atatcgccca ggtgatcttc
    20161 gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc
    20221 gatacccgca acggcagctt tgatcggcac accggcgtcc atcagcgcca gagacgcgcc
    20281 gcacacggaa gccatagaag aggaaccgtt ggattcggtg atttcagaca caacacgtac
    20341 ggtgtacggg aatttgtcca tatccggcat tactgccagc acgccgcgct tcgccagacg
    20401 accgtgacca atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta
    20461 cggagggaag ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag
    20521 aacctgcgcg tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg
    20581 ggtgaacagc gcagaaccgt gagtacgcgg cagcacacca gtacgcacat ccagaccacg
    20641 gatcatatct ttttcacgac cgtcgatacg cggttcgccg gccagtacgc ggctacgaac
    20701 aacatttttc tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc
    20761 ttcagcaagc agtgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc
    20821 ttgtttgtcg gtgatgcggt aagcatcgct cagacgagct tcagccagcg cagcaacgcg
    20881 cgcgttcagc gcttcgttta ccggctccgg ctgccaatcc caacgcggtt taccggcttc
    20941 tttcaccagt tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacaccac
    21001 tgcgcccagc atttggtctt cgctcagcag ttcagcttca gattcaacca tcagcacggc
    21061 agcttctgta ccggcaacaa ccaggtccag tttgctctct ttcagttcgt cctgagtcgg
    21121 gttcagtacg tactggtcat tgatgtaacc tacacgggca gcaccaatcg ggccattgaa
    21181 cggaatacca gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt
    21241 aacctgcggg ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc
    21301 cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct
    21361 tgggcggcct tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc
    21421 ctgatagtta acggtcagcg ggaagaagtc ctgacctggt ttggcttttt tctgaccaac
    21481 aacggtaaca aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg
    21541 agccatcatg ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat
    21601 cggattaagc aatgtaatat cctttctctt tcttagacag taccttacgg tactggtgtt
    21661 aatacccgat cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg
    21721 gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata
    21781 cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt
    21841 ttctgaaact cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg
    21901 tgctacgtct ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag
    21961 cagaccacga cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt
    22021 gatctgtgca gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg
    22081 accaaactca gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca
    22141 aagtatatag aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca
    22201 aagtgttaaa caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat
    22261 cgcaagacgg ttaacattac gccgggtatt caaccaccag gcgacgaggc gcaacgcggc
    22321 cttcatcgtc aatttcgccc ataccgataa acttgccgtt ctcaccctct gtaacgcgaa
    22381 ccagtccttc cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag
    22441 aagacgttaa cggaagattc accactgggt agtccgaagc tggactgtcc attggcatca
    22501 gtaatgggtc aagtaactcc gcagccggaa tatcctgctg ttcggcttgc tcaaccagtt
    22561 cacgcagatg ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac
    22621 gcaagtaaat aacatgcgcg ccacaaccga gtttttcgcc gagatcgtca atgatagtgc
    22681 ggatataagt gccttttgag cagtgaattt ccagctccag ctcattgcct tcatggcgaa
    22741 taaacagcaa ttcataaacg gtaatcgggc gcgcttcacg tggaacttca atgccctgac
    22801 gcgcgtattc gtacagtttt ttgccctgat atttcagtgc tgaatacatc gaaggaatct
    22861 gttcgatatc gccacggaag gtatccagcg ccgccgccag ttgctctgca ctaaaggtta
    22921 ccggacgttc ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac
    22981 gcgcaatgac ccgataacgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt
    23041 ccccgaggca aattggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg
    23101 cacggttggc gttataaatc cgtttcactt tttgcagcgc atcgttgctg gacatcccct
    23161 gcggtttatc cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca
    23221 ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct
    23281 ggtcaccagg tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag
    23341 ttccggcacg atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc
    23401 ttcctgcaac gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt
    23461 cacatatact ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat
    23521 catgcccaga cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat
    23581 ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa
    23641 aaaaggggct gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg
    23701 cctgataaga cgcgccagcg tcgcatcagg caatccatac aggatgcggc ataaacgcct
    23761 tatcccgcat ggaaccctaa aaaccttaag cgatggtacg ttggatctcg atgatttcga
    23821 atacttcgat cacatcgcca gtgcggacat cgttgtagtt cttaacgccg ataccacatt
    23881 ccataccgtt acggacttcg ttaacgtcat ctttgaagcg tcgcagggac tccagctcgc
    23941 cttcgtagat aaccacgttg tcgcgcagaa cgcggatcgg gttgtgacgt ttaaccacac
    24001 cttcggtaac catacaacct gcgatagcac caaatttcgg tgatttgaac acgtcacgaa
    24061 cttccgccag accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg
    24121 ctttcacttc gtcgatcagg ttatagatga cggagtagta acgcagatcc aggctttccg
    24181 cttcaatcac tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcgt
    24241 tggacgccgc agccagggta gcgtcggttt cggtgatacc acctacgcca gaaccgatga
    24301 tcttcacttt aacttcgtca gtagacagtt tcagcaagga gtcagaaatt gcttccacag
    24361 aaccctgtac gtctgccttc aggacgatat tcacttcgtg aacttcgcct tcggtcatgt
    24421 tggcgaacat gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt
    24481 taccctgacg ataaagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt
    24541 catcacccgc agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg
    24601 cttccagcac ttcctgaccc agttcgttac gcattgcacg aacacgaccg tattcgaagc
    24661 cacacagaac gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg
    24721 gaccacgacc tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta
    24781 ccgctttcag ctccagaact tccgcctgca gcaggatagc atccagcagt tcatcaatac
    24841 cggtacccgc ttttgcagat acgtgtacga actggctttc gccgccccac tcttctggca
    24901 ggatgccgta ctgggagagt tcgttcttaa cgcgatccgg atcggcttct ggtttatcga
    24961 ttttgttcac agcaaccacc accggaacac ccgccgcttt cgcgtgctgg atagcttcga
    25021 tggtctgcgg cattacaccg tcgtctgcag caacaaccag gactacgatg tccgttgcct
    25081 gcgcaccacg tgcacgcatt gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga
    25141 tcatgccgtt ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt
    25201 cgccagaggc cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa
    25261 cgtgacccat gatggtcaca accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt
    25321 cgctcattac cgcctcttcc agctcgtttt cacgacgcag gataactttg tggcccatct
    25381 cttcagcaac cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc
    25441 ccagtttcat catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt
    25501 cgccaacggt gatggtttcg ccgatcacaa cgtcacggtt aacggcctga gctggcttct
    25561 ggaagccttg ctgcagcgaa gaacctttac gttttccgcc cttaccgcca cgtactgctg
    25621 cacgagcttc ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg
    25681 ctgctttcgc gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt
    25741 ctgcctggcg agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt
    25801 ccgcgttatc agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac
    25861 gtgcttcttc ttcgagttta cgacgcgctt cttcttcggc tttacgcttg agctctgcag
    25921 cttcctgctc acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat
    25981 tgctcacttt gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag
    26041 cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg
    26101 cctcacgacg ggcttgctct tccgcttcac gctgcgcttg ctcttccgct gcaaggcgct
    26161 cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg
    26221 atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg
    26281 tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt
    26341 gtgcagacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca
    26401 ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc
    26461 tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag
    26521 ccataatcag tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa
    26581 tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca
    26641 aatcacgatc taccccttca aggttcagca gatcatcagc cggtttgtta tcaccgaggc
    26701 tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt
    26761 caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca
    26821 gttcttccag cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcatcgatgt
    26881 cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca
    26941 ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt
    27001 tctgaccgtt acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg
    27061 tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca
    27121 cgaactgcgc cgggttatca tcccacagga cgatatcgat acgctctccg cccagttcag
    27181 tagaaaccgc ctgaacacgc gcgccacgca tacctacgca agcacctacc ggatcgatac
    27241 gtttatcgtt ggttttcacc gcaattttcg cacgagatcc cgggtcgcga gccgctgctt
    27301 taatttcaat cacttcttcg ccgatttccg gcacttcaat acggaacagt tcgatcagca
    27361 tttccggctt ggagcgggtg acgaacagct gcgcgccacg cgcttccggg cgaacggaat
    27421 agagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc
    27481 gcaggatcac ggcttcagcg ttgttgccca gatccagaga gatgttgtcg cggtttactt
    27541 ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg
    27601 cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac
    27661 gatcaaaggt aacagactca atctgatctt caacgtaatc gcccaagttc aggctttcat
    27721 cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa
    27781 ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcgaacgt
    27841 cgatttcttg ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga
    27901 aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt
    27961 ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg
    28021 ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca
    28081 tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt accgccatac
    28141 ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg
    28201 ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg gggtcttcaa
    28261 catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat
    28321 cttcactatc aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa
    28381 ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta
    28441 atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag
    28501 tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa
    28561 ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc
    28621 ttcacagtat atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac
    28681 cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa
    28741 cgtggcaaat tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc
    28801 gagacgggac gtaaaatctg cgggcattat agtgatccac gccacatttt gtcaacgttt
    28861 attgctaatc acgtgaatga atatccagtt cactttcatt tgttgaatac ttttaccttc
    28921 tcctgctttc ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg
    28981 ataaaagatg attaaatgaa aactcattta ttttgcataa aaattcagtg agagcagaaa
    29041 tccaggctca tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca
    29101 tctcccggta ggtcaacgta ttggtatcgc tttttccggc ggtctggaca ccagtgccgc
    29161 actgctgtgg atgcgacaaa agggagctgt tccttatgca tatactgcaa acctgggcca
    29221 gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa
    29281 cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg
    29341 tggcgcattt cataacacca ctggtggact gacctatttc aacacgacgc cgctgggccg
    29401 cgccgtgacc ggcaccatgc tggttgctgc tatgaaagaa gatggcgtga atatctgggg
    29461 tgacggcagc acctataaag gaaacgatat cgaacgtttc taccgttacg gtctgctgac
    29521 caatgctgaa ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg
    29581 cggccgtcat gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt
    29641 cgaaaaagct tactccacgg actccaacat gcttggtgca acgcatgaag cgaaggatct
    29701 ggaatacctc aactccagcg tcaaaatcgt caacccaatt atgggcgtga agttttggga
    29761 tgagagcgtg aaaatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt
    29821 ggcgctgaac ggtaaaacct ttagcgacga cgtagaaatg atgctggaag ctaaccgcat
    29881 cggcggtcgt cacggcctgg gcatgagcga ccagattgaa aaccgtatca tcgaagcaaa
    29941 aagccgcggc atttatgaag cccctgggat ggcactgctg cacattgcgt atgaacgttt
    30001 gttgaccggt attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt
    30061 gggccgtctg ctgtaccagg gacgttggtt tgactcccag gcgctgatgc tgcgggactc
    30121 tctgcaacgc tgggttgcca gccagatcac tggtgaagtt actctggagc tgcgccgcgg
    30181 gaacgattat tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct
    30241 gacgatggaa aaaggcgact cggtgttctc gccagatgat cgtatcggtc aactgaccat
    30301 gcgtaacctg gatatcaccg atacccgcga gaaacttttc ggttatgcca aaactggcct
    30361 gctctcctcc tctgccgctt caggggtgcc gcaggtggag aatctggaaa ataaaggcca
    30421 gtaattcgac atcaaccctg cccttcaggg gtggggttac cttgattatc ctctgtaaac
    30481 tcctttctga gcatcacata atcgaaaaca tcatggtttg catccacaac ccgcgagtct
    30541 cctttatagt gtgcaatcac ttcctcaagc gtttgcggct gttccggctt tgttaccccc
    30601 atccacgcat taataatgta attattgtaa gctgtcgaaa agatgttgtt ttccgttgtg
    30661 cgatccacgc catccccaag aacggggcta taccagataa acatagggac atgatatgcc
    30721 tgctggctag cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcgcgttcc
    30781 aggccgtggt cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaacc
    30841 tgacctagca aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc
    30901 tgcggttgaa acacagcgct ggattgcgga taggcgctac aagccggttc atggcttcca
    30961 tttaaatgaa gaacaatcag tttcttttgc tgcgtctttt gctgtaacgc ttgcgataaa
    31021 tgcggcaaca acaattcatc aaatcctctg acatagactg tttccatggc gcgcatggcg
    31081 atactggtaa ctgctgtacc attctgccga aaagcggatt gcgagcttag ccagaaagtc
    31141 tgaaatcctg cttgattagc catattaata atgttgtccg gataattatg aatgtcatga
    31201 ctcaaaacag aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta aggtgcaccg
    31261 cttattgctt gattaaacag tttgatctgc tttctttgtg cttcgacttg cggtgtcgta
    31321 gagtgtgtat atccatacaa agacatattg tcgacacgta cagactcccc cacaatcaac
    31381 acgtaggtat caatacctgt atccctgact gataattgaa aatacggcac cgtgtttgca
    31441 attgagagta atctttgatg ctctttcgct gctaaagcaa aataattgag attgaaaaac
    31501 ggcgtatagg tagcaaatcg cgacgctagt atatatggac tgaacgcctc tttatttttt
    31561 gcatctttat aagcaaattg acaagcggaa aataaactgc ccgaaatgac aatcagcaat
    31621 aatattcctg tcactttttt tgtcggcaag gaaacatcat attttattat tactgccaaa
    31681 aaaagaaggg ataaaaaggc aaaagcacat agataaggga tatacattcc cagcattttg
    31741 acaacttcat ccggatcact ctgcagcaca ctaatcgcaa atccatcatt gaaagttgta
    31801 ccaaagatac accatgaata gaggctaata ctcatatctg ccgcagtcag taacacaaat
    31861 ggtgcggcaa taagcaggcg aagcggaagg gatggtatac gtttaacaac caggatggtc
    31921 agaacgaaga acagcaatcg ctgaaccata tgcgctccgg aagaagcgac taagttcgta
    31981 ataccaaaaa taataacaca aagatacaac aaccaatgag atttaaaact tttaacaaat
    32041 ttgttgaata ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa
    32101 tgttcaattt tatatcagac atgtttttca actctaatcc atatcagacc gaaacactgc
    32161 tagatttttt ctttttaaat gatgcggcca ttgcgttctc agtaattaaa taacattatc
    32221 aatgcgtctc taaacattac gcagaacgta gccatcagta gttatactga caggggcagg
    32281 atggtttatc gagaagtaaa catatccgta ttcgtaccgg aaatggagtt gacacaataa
    32341 agtgccaatt atgtcagcag aagggaaaag cttataacta aggcataccg gcaccatcgt
    32401 ttcaaggtac cagctacgag taaaacaact ggacgagata cagatacctg acaaccattc
    32461 ctcagaccag gaccaaaacg aaaaaagacg cttttcagcg tctttctgga atttggtacc
    32521 gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct
    32581 accaattcca ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct
    32641 tagccggagc agctggctga gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt
    32701 cgctaccttt attggttttg ttgctattga tgttacccag caccagactg atgatgaaga
    32761 ataacgttgc cagcagcgcc gtcatgcgag tcatgaagtt accagaacca cttgaaccaa
    32821 acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct
    32881 gcagcatgat cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt
    32941 cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag
    33001 cgggaatatt taacctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct
    33061 tcgcaagggc aattttattg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg
    33121 ttcgctgaga aaaaaggcga caatcgccgc ctttttagcc agccatttat cgttttaaac
    33181 ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc
    33241 ttccaccatc acgcgaatta acggttcggt gccggattta cgcaacagca cacgtccacg
    33301 gctacccagc gccgcttcaa cttctgcggt cacagctttg actgattcat gctccaacgg
    33361 atcgccgcta cctgcggtgt aacgcacatt aaccagaatc tgcgggaaca ttttcatgcc
    33421 gctgcaaagg tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaagcc
    33481 agcaacaatg ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggagttctc
    33541 tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt
    33601 cgcgcgcgca aatggaatac ctaactgttt cagcgccagt tcaagcccca tgttgctcat
    33661 caatgtaccc acagcaccac cacgcagctg gccctgacga agaccttcac gcgcgatgat
    33721 atacatgatc tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc
    33781 gccgtcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag
    33841 cgcgcgaacg tcggtagccc ccacttcggc attgatattt acaccgttcg gctcacaacc
    33901 gatagcgata acgttcgccc ccagttcacg cagcacgttc ggtgcgatgt gataagtcgc
    33961 accgtttgca caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt
    34021 ggctttgcaa aactcgatat aacgacccgc ggcatcaacg atacggctgg ctttacccag
    34081 ttctgccgaa tcaacacagc tgatctcttt ttccatttcc gcttcgatgg cttcttcaac
    34141 tgcatccggc agcttagtac cgtcaataga aaagaattta atgccgttat cgtagaatgg
    34201 gttatgcgat gcagaaatta caattccagc ctctgcgcgg aaggtacgcg tcagataagc
    34261 cacggccggt gttggcatcg ggccagtgaa gagcgcggaa agaccggcag ccgcaagacc
    34321 cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat
    34381 cttacgagag ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa
    34441 atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt
    34501 acgattactc atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg
    34561 cttctacggt ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa
    34621 tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcaattgcc
    34681 caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag
    34741 ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt
    34801 cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa
    34861 agtagcgatt cacttctgca aagacatcgt catacttcgg cgcttcctgc atggtttttg
    34921 gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag
    34981 caccaggttc ggaaagggag cggatatcgt taataatgtg agcgccaact ttcgctgact
    35041 cacggatgac ttctggtttg gatgtatcta ccgagatcca gacttcgaag cgttgagcaa
    35101 ttgcctcaac cacaggaata acacgttgca actcttcttc aacgcaaact tccgccgccc
    35161 ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat
    35221 tcgcatgttt caccgcatct aacagcgagt tatgcgtgcc accatccgaa aaggaatcag
    35281 gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg
    35341 caaagagttt catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc
    35401 cccggggttt tcggtacaaa tacagtcatc tgatacggga acttacttgt cgcctaactg
    35461 ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt
    35521 tggactacca ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg
    35581 acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt
    35641 catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc
    35701 acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc
    35761 atcggacata tgtttcgctt tcgctacgct acggccgagg aacacttcac cttcttcttc
    35821 cgcgtacagc agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc
    35881 caggttggtc gcaactttaa tatcgttgga cgccccggta gaaacatgtt ccggtccgta
    35941 gatgatctct tccgccagac gaccaccgta cagcgtggag atctgacttt ccagtttctg
    36001 acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca gcgcacgacc
    36061 gcgtgggata atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat
    36121 cgcatgaccc gcctcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga
    36181 gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgaaac
    36241 aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc
    36301 agcaccggag aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa
    36361 tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc
    36421 aaccacaacc tgacggtcga aacggccagg acgcagcagg gctgggtcga gaacgtccgg
    36481 acggttagtc gcagcgataa cgatgatacc ttcgttacct tcaaaaccat ccatctcaac
    36541 cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccaagc ctgcgccgcg
    36601 ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc
    36661 ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc
    36721 agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaattgctt tcgccagcaa
    36781 cgttttaccg gtacccggag gacctaccat caggacgcct ttcgggatct taccgccgag
    36841 tttctggaag cggctcggct cacgcagata ctcaaccagt tcagcaactt cttcttttgc
    36901 ttcgtcgcag cccgcaacgt cagcaaaggt ggttttgatc tgatcttccg tcagcatgcg
    36961 cgctttgctc ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat
    37021 gaagaagatc cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc
    37081 cagcaggctt ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt
    37141 atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt
    37201 ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc
    37261 ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg
    37321 gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc
    37381 actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat
    37441 ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac
    37501 aatatacact tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt
    37561 aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac
    37621 tacaaaactg ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag
    37681 atacatggca cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga
    37741 catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc
    37801 atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc
    37861 gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc
    37921 cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg
    37981 tatttcatca agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac
    38041 atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc
    38101 tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg
    38161 cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag
    38221 caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc
    38281 cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga
    38341 ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg
    38401 aaactaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg
    38461 tcatcggtaa aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac
    38521 gctaagatta tcctaaagtt acacacattc gctgtgtaaa acgaggggtt ttccgcaggc
    38581 aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct
    38641 ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa
    38701 ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt
    38761 tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag
    38821 cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatcgaga ttcagcaccg
    38881 ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct
    38941 gcgcgttcga cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt
    39001 cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga
    39061 tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt
    39121 tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc
    39181 tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg
    39241 ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat
    39301 tttgatagtc attttaccct ggagttcccg aagggtcatc gtttacttta tagggcgttg
    39361 cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat
    39421 ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt
    39481 acattactca actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt
    39541 cggcccccgc tattgattac cacagtcagc agatggcgct acccgccagt acgcagaaag
    39601 tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttt accacgacgc
    39661 ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtt gcgcgatttg
    39721 gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat ggtcacgact ttgaaaaaat
    39781 ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg
    39841 ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgca ccgcctgccg
    39901 ccgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg
    39961 atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca
    40021 ccctcccccg tggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggtgacc
    40081 tgaaccgctt tacgctaacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt
    40141 ttgccgtgca ggatggtgcc agctatgccg gggcaattct gaaagatgag ttaaaacagg
    40201 cgggtatcgc ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg
    40261 tagttgccag taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt
    40321 cggacaacat gattgccgat acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc
    40381 ctggaacatg gcgggccgga tcggacgccg tgcgtcagat cctgcgccag caagccggtg
    40441 tcgatatcgg aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg
    40501 cccccgccac catgatgcag gtgctgcaat atattgccca acacgacaat gaacttaact
    40561 ttatctccat gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc
    40621 atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata
    40681 acctggcggg attcattacc acagcgagcg ggcaacgaat ggcgtttgtg caatatcttt
    40741 ctggctatgc agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg
    40801 aaagccgttt gtataaagat atttatcaga ataattagcc taaagagaaa ccccggcaca
    40861 tggccggggt tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc
    40921 gtcccagtcg tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctgacg
    40981 gtgataatca tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac
    41041 gaccgggttt tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc
    41101 gctcgccgca gagatcagat aatatttatc ttcccagccc agcgcttcag cgatcgcttt
    41161 cgctttctct tcggcttctg ccttatccag cagatcgatc ttgttgaaaa ctaaccaacg
    41221 cggtttcgcc gccagatcct ggctgtattt ttccagctca ctgataataa tacgcgcgtt
    41281 ttcaaccgga tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca
    41341 acgttccagg tgcttcagga agcgaatgcc cagacctgcg ccttctgcag cgccttcaat
    41401 cagccctgga atatcggcaa caacgaagct cttttcgttg tccattcgca ccacgcccag
    41461 gcttggtacc agagtggtaa acggataatc cgccactttc ggtttagccg ccgatacagc
    41521 acgaataaag gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag
    41581 catcagctcc agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg
    41641 tgtacggtta actgacgatt tgaaacgagt gttgcccaga ccgtgccagc cgcccttagc
    41701 aaccagcaga cgctgaccgt gtttggtcat atcacccatg gtttcaccag taccctggtc
    41761 gattacacgc gtacctaccg gcactttaat cgtgacgtct ttaccgcgct tacctgtaca
    41821 gtcgcggctt gcaccgtttt gaccgcgctc tgcacggaaa gatttttcaa aacgataatc
    41881 gataagcgtg ttcaggttct cgtcggcttc catccatacg tcaccaccgt caccgccgtc
    41941 gccgccatcc gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt
    42001 accgccatca cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc
    42061 cgtaaatcat tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc
    42121 ccaaatacga tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag
    42181 ataacctaaa aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag
    42241 atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc
    42301 ctgccagcga gccatcgctt ccgccagggc gccatatcct accaatgtat tcagtccgca
    42361 aaaaattaaa caggcgagct gccagtggct aagctgcgct atcactccag gatttgccag
    42421 agggaagagc gcaattgtac ataaagtgta cagcaaaaac aggatctgcg gtgaggccag
    42481 ccgacgcaat aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac
    42541 cccaaagata actccccagg tgtaatcggt gagctttgta aatatctcga ccagactggt
    42601 gttaaaaaac atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtactgcg
    42661 cattttctct ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc
    42721 aatcacctgc gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt
    42781 cccaaacagc ccggcggtcg ccactgctaa caaaatcaac cagcgtggct tacgaaacac
    42841 gcgtaatggc ggcaaccgct tcttcaccgc aagaatggca ccaaggccaa tactcgccat
    42901 caagaaacgg taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc
    42961 gattggcaac gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc
    43021 ctgctgcttc ataccgtatt ccctgcaaag cgcatatgcg gattaacgtc gcaccatgcg
    43081 catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt
    43141 taccgggacg cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc
    43201 tttaacttcg aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca
    43261 acctacgtta gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc
    43321 cagaacggat tcaccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc
    43381 gttacgtgtg gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag
    43441 gcgctgatgc cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga
    43501 tagtgtttac gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca
    43561 acttcagctt tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg
    43621 tttgcgatca tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt
    43681 tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc
    43741 gcgtacatat aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa
    43801 atattcacaa tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc
    43861 aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag
    43921 tacaatcacc ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa
    43981 tcggggcgaa aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc
    44041 aagatatggc gggtgttaat gcgtcaatcc ttgagcagct taattccgac gtccaactga
    44101 tcaatcagtt aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg
    44161 ctgtactggc tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc
    44221 tgatcgagtt tatccacacg gcgactctgc tacacgacga cgttgtggat gaatcagata
    44281 tgcgcagggg taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag
    44341 gcgattttat ttatacccgc gcgttccaga tgatgaccag cctcggttca ctcaaagtgc
    44401 tggaagtcat gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga
    44461 acgttaacga tccggacatc actgaagaaa actacatgcg cgttatctac agcaagactg
    44521 cgcgtctgtt tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg
    44581 aggagaaagg cctacaagat tatgggcgct atctcggcac tgctttccag ttgatcgacg
    44641 atttactcga ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga
    44701 acgaaggtaa accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg
    44761 cacagatgat tcgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc
    44821 tggaagcaat gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag
    44881 cagacaaagc catcgcagcg ttacaggtgc tcccggacac cccgtggaga gaagcactta
    44941 tcggcctcgc gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgca
    45001 gtgcgcggga tgattccagt aaattccata aaacacttat tcagctctat aactcctgaa
    45061 aaacagggct gtgtatcttt atatattctg aatattcaca ctctttacag gaacttttta
    45121 gagcaatagg ccatcaggag tatagtgatg ctcgacagaa acagaagaag tattctgaat
    45181 gaaagcgact aatttaagga gtgaggaaag atggaaagta atttcattga ctggcatccc
    45241 gctgacatca ttgcaggttt gcgcaagaag ggaacgtcaa tggcggcgga gtctcgcaga
    45301 aatggtttga gttcctcaac gctggcgaac gcattatcac gcccatggcc gaaaggagag
    45361 atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc acgctaccat
    45421 gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta cactaaaccg
    45481 aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc agacaattac
    45541 tcgcctttca cacgctcaat atttgcacct aaagcgcgta gtttgtcttc aatgcgttcg
    45601 tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc aatacagcca
    45661 gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc gccagaaagt
    45721 ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcgtgcgc gcccatacgg
    45781 ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat gaatccggtc
    45841 ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga
    45901 tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc caggctaatc
    45961 cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca gtttcgccag cacggcgtcc
    46021 agagtatctg gctgcgcgtt acggcagata attttgccgc gagagatcgc cgccgccacc
    46081 aggaaagtac cggtttcgat acgatccggc agcacgcgat agacaccgcc gcctaaacgt
    46141 tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc acccagcgta
    46201 atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg
    46261 gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac gctgacttta
    46321 tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac gtaaccttct
    46381 tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc aaccggacgt
    46441 gcgccgatcg tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg cgctaccagc
    46501 ggccccagcg cccagataga agcacgcatg gttttaacca gatcgtaagg tgcgcagaat
    46561 acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt cgcacccagc
    46621 tggcttagca gcttcattga tgtatcgacg tcttttagtt tcgggacgtt ctggatctct
    46681 accggttctt ccgccagtag tgcggcaaaa aggataggca gagcagcatt tttagcgccg
    46741 gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt atccatttag
    46801 tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc tcaaaagccg
    46861 ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga cacagcatga
    46921 atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg ttttttaacc
    46981 cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct gccatcgccg
    47041 gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat ttcattattt
    47101 tccatgggat cttcaatcat cagttaataa atcagcgaaa catcttagag caaagttgcg
    47161 ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac ttgcaggcag
    47221 gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg aggcagaaca
    47281 tcagcaggca aattatacaa tttcgccagg gtatacactt tgtcgtttac cccctgaagc
    47341 gtcacattgt tgccctgctt tttcgccaga tcgataagat ggagcagcag tgccagtcca
    47401 cccgtatcca cgcgagacac acggctaaga tcgatgctgg taatcccctt caccgcttcc
    47461 tcacgtattt cccaaagcgg tagcaaaacg tcctgatcca gctctccgga taacgccagc
    47521 gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct tccagagtga
    47581 ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca
    47641 gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca gcaatcatgt
    47701 cgtaagcctg ccagttgccc gtctgggagt ttttacgcca ctggaagtcc agacgcaccg
    47761 gcgggcggcc attcgggtca ataatggtaa cgcgaatcgg cacaatggtt ttatcaccca
    47821 gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc gcctgaccgt
    47881 aagcctgctt caggtactca cggaaagcgg caaagtaagc atcacgttga gctggggtcg
    47941 cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt acgtatggca
    48001 acagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt tgcggttgct
    48061 cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta tacggattgg
    48121 tctggtctgc cgcggttgcc gcactcagcg gtgcaatcac cagcaaagcg accatcatta
    48181 aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca ggttcagtgg
    48241 tttcattatt acctggcgca gcagctggcg catcgccact attcttattg tcatcgcctt
    48301 tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac ttggtgtcct
    48361 gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca aaaccgacgt
    48421 ttaatgccag atattgttcc cccagcaggc cggaagtacg aatgctcagc gaactggtat
    48481 caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga taggttttcg
    48541 ggtccagcgt aatatccgcc acccggccca caacaacgcc accaatgctg accggagagc
    48601 gggctttcag gccgccaatg ttatcgaacg tcgcataaag cgtataggtc ggttcagtac
    48661 gtatagacgt cacgttcgct gccttcaggc aaacaaacag cgccgcaagc agtgctgcta
    48721 ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca attcccaaac
    48781 atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg gacaacggtg
    48841 cgagtggttg cccggctaat cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac
    48901 gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt gacgagatcc
    48961 atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccactatc aatgcctttc
    49021 caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaagat aaccgtcaac
    49081 agtggtaatg aaataactcc agcccagaaa cggggagaaa taacccgacg cagcggatcc
    49141 accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg
    49201 gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg ccccagttca
    49261 cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata agtggtcaga
    49321 accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac aacaataatc
    49381 agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggtgc atgtttgcga
    49441 aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc gaacgttctc
    49501 agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat gagtggctta
    49561 actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg gaacaggccc
    49621 gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct gcaacgcctg
    49681 ggcactacca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat ccgcaatact
    49741 taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg cgctgttcag
    49801 ctcagaaatc agcttcacca gcacgcccat ggtaatggga tcttgcccaa caaagggctc
    49861 atcaaacatg atgagatccg gctccagcgc aatcgcacgt gccagcgctg cacgccgcgc
    49921 catcccaccg gaaagttcag aaggcataag tttagccgct ccacgcagcc ccacggcctc
    49981 cagcttcatc atcaccgtac tatgcaacaa tggcgcggga agttgggtat gttcccgcag
    50041 tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg actgaaataa
    50101 catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg gaatattctc
    50161 accatcgaaa agaatctcac catgatctgg tgcgatttgc ccgccaatca gacggagtag
    50221 cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc gcggcacggt
    50281 cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat cgcgcatatc
    50341 gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg attttctaag
    50401 catggcgctc aatttaacct tgaacccaac atatttacag aatattacct gccgtggtta
    50461 gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa gaatctgtta
    50521 cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga cgttagatgc
    50581 ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt gccgaccgcc
    50641 tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgttg atcatcggca
    50701 tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt gccgcgtctt
    50761 tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt atcaatatat
    50821 tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattct gatgttctac
    50881 gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta ctctatgacg
    50941 gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta tggctgctgt
    51001 tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg accagagagc
    51061 agcttgcaga gctgccgcgt gaaggtggat tgcccgtcgc atttttatgg ctcggcatcg
    51121 cactgatcat tatgccagtg gccacgcgga tggtagttga taacgccacg gtgctggcga
    51181 attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc ggaaccagcc
    51241 tgccggaact ggcaaccgca atagcagggg ttcgcaaagg tgaaaacgac attgcggtcg
    51301 gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta cccgcgctga
    51361 taacgccagg agagattgat ccactggcgt acagtcgtga ctacagcgtg atgttgctgg
    51421 tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc cgtggtgtag
    51481 gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg ttatcgccaa
    51541 tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc gggttttgac
    51601 tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc ggagcttgat
    51661 caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg taaagggaaa
    51721 gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc agcaacattt
    51781 gccagcactg gtacaccttc atttttcgtc catcctggtg aagccgcgca tggtgattta
    51841 ggcatggtca ccccgcagga tgtggtgatt gctatctcta actcaggtga atccagcgaa
    51901 atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg catcaccggt
    51961 cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa agtagcgaaa
    52021 gaagcctgtc cgttagggct ggcaccgacc agcagcacaa ccgccacgct ggttatgggc
    52081 gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga ttttgcgctc
    52141 tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga tattatgcat
    52201 acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc attgctggaa
    52261 gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat gattgaaggc
    52321 atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt tcgtcagtta
    52381 agtattgccg atgtgatgac gccgggggga atacgcgtgc gcccaggcat tctggccgtt
    52441 gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc cgatggcgac
    52501 catttactcg gtgtgttaca tatgcatgat ttactgcgtg caggcgtagt gtaaagattc
    52561 aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac ggccctgtca
    52621 gcgccgacgt tatggcaaaa gcagagaaca ttcgtctgct gatcctcgac gttgacggcg
    52681 tactgtcaga tggcctgatt tatatgggca ataacggcga agagctgaaa gcgttcaatg
    52741 ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc gctatcatta
    52801 ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc actcacttgt
    52861 atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa ctggcgattg
    52921 ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta atggaaaaag
    52981 tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc gccgattacg
    53041 taacgcgcat tgctggcggt cgtggcgcag tgcgagaagt ttgcgactta ttactcctgg
    53101 cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca gacgttgggt
    53161 tatcattgtg ctatcactgg cggttctggt gatgatcggc attaatatgg ccgaaaaaga
    53221 cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg agcatacgga
    53281 cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc aacacgttga
    53341 atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca cgtttgataa
    53401 ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca atgaccggat
    53461 gctctattta tatggacacg ttgaagtcaa tgcactcgtg ccggactctc aacttcgcag
    53521 aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct ctgaagacct
    53581 cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg gcaacttacg
    53641 cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc aaaacaaaca
    53701 aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt ccggcatttg
    53761 ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa tctcttgata
    53821 tgcaaggcaa cgtggttacc tttaccggta atgttatcgt cacccagggc accatcaaaa
    53881 ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa gaagtgattg
    53941 acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa cccgttgaag
    54001 gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg acgggtaatg
    54061 cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac ctggtgaaag
    54121 agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt ctggtgccgt
    54181 cgcagttgca ggacaaaaac aataaaggcc agaccccggc acagaagaag ggtaattaat
    54241 tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc cgtcgcgtgg
    54301 tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg gggccaaacg
    54361 gtgccggtaa gaccaccact ttctacatgg ttgttggcat tgtgccgcgc gatgcgggca
    54421 acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc gcgcgccgcg
    54481 gtatcggcta tctgccgcag gaagcctcca ttttccgtcg cctcagcgtt tacgataacc
    54541 tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa gaccgtgcga
    54601 acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg cagtcactct
    54661 ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat ccgaaattta
    54721 ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac attaaacgca
    54781 tcattgagca cctgcgcgac agcggcctgg gcgtactgat cactgaccac aacgtgcgtg
    54841 aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg atcgcccacg
    54901 gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt ggggaagact
    54961 tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga ttctgaacat
    55021 gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac agctccaaca
    55081 ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caagagctac agcaggcgct
    55141 ggagagcaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca cccgcgaaac
    55201 gcaagacagt gaaacactgg acaccgccga cgcgctcgaa caaaaagaga tgccggaaga
    55261 gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat ccggcaccag
    55321 cggtgactac attgatgacg agctgccggt ctatcagggc gaaacgacgc agaccttaca
    55381 ggattacctg atgtggcagg tcgagttgac accgttttcc gacactgacc gcgcgattgc
    55441 tacctctatc gtcgatgccg ttgatgacac cggttatctg actgtcccgc tggaagatat
    55501 tctcgaaagt atgggcgatg aagagattga catcgacgaa gttgaagccg tccttaagcg
    55561 gatccaacgg tttgatccag tcggtgtggc ggcaaaagat ctgcgtgact gcctgctgat
    55621 ccaactctcc caattcgata agaccacgcc atggctggaa gaggccagac tgatcattag
    55681 cgatcatctc gatctattag ccaatcacga cttccgcact ttaatgcgcg tcacgcgtct
    55741 gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc cgcgccccgg
    55801 gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg tgcgtaagca
    55861 taacggccac tggacggtag aactcaacag tgacagcatt ccgcgtctgc aaatcaacca
    55921 gcactacgcc tcgatgtgca ataacgcgcg caatgatggt gatagccagt ttatccgcag
    55981 caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg atacgctact
    56041 gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag gtgaagaata
    56101 tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg aatcgacgat
    56161 atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg aactgaaata
    56221 tttcttttcc agtcacgtca atactgaagg cgggggtgaa gcctcttcca cagcgattcg
    56281 tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga gcgacagcaa
    56341 gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg ttgcgaagta
    56401 ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt gacccaaccg
    56461 ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc accgaggcac
    56521 tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac cgaatcaacc
    56581 aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca acactgcatg
    56641 taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct gccattgatg
    56701 gcttaattga taagctggca cgccagttga ccaaacataa agataaactg aaacaacact
    56761 aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac gggccatttg
    56821 tacggttaat gctccgaacc tgttccactg tttgagtggg caggttctta ggtgaaatta
    56881 tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa tgtacgcgaa
    56941 gccgcgtcca ctgccagagc aaaaaacgcg ccctggaaat catcagcgag ctggcggcga
    57001 aacaacttag cctggcgcct caggtggttt ttgaagctat cctgacgcgg gaaaaaatgg
    57061 gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa gaagatactc
    57121 tgcgtgccgt tggcgttttc gttcagctcg aaacacctat agctttcgat gccatcgaca
    57181 accaaccggt ggacttactt tttgccctgc tggtgccggc agaccaaact aaaacgcacc
    57241 tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc cgccgtttac
    57301 gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa ggtactccgg
    57361 atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa cagtacatgg
    57421 tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg cgtgcgctgg
    57481 aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc gatctggctc
    57541 gaactctggc cgatcgagag atttctgccg ccgtcagcat tgatgttcgt aatatgccgg
    57601 agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc tcaccgcaac
    57661 tactgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac acgcgccgac
    57721 tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa gaaagcgatt
    57781 tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa atgtccgttc
    57841 acgagctggc agaaatgctg cgtacccgtc tgttgggtaa acgtgaacgt gaactgacca
    57901 tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat tacgtctttg
    57961 acgtgcgctt cttgccgaac ccacactggg atccgaaact gcgtccaatg acaggtcttg
    58021 ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt atctaccaga
    58081 cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt agctacctga
    58141 cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca gagcaactgg
    58201 cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcggacg ctggaaaaac
    58261 gtaaaccatg accgtcaagc aaactgttga aattacaaac aagctgggca tgcatgcccg
    58321 gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc tgttacgtaa
    58381 tgacgaaggc accgaggctg aagccaacag cgttattgcg ctgctgatgt tggattctgc
    58441 caaaggacgg cagattgaag ttgaagcgac cggtccacag gaagaggaag cactggccgc
    58501 cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac tttgacattc
    58561 aaatcccttc aagctccccc ggcaataaga aaagcgcgta ctgaaatcgt tatcaatcaa
    58621 cgtcgattgt acatttcgtg cgctttttac tttcagaaga accttaagaa aaccttaaga
    58681 agcattgttt agatttcgtt taagttaatc gaccatactg gagatcgtca gaaaatattt
    58741 ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg aagacatcgg
    58801 aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta ccatcgtggc
    58861 gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct tggtcgccgg
    58921 ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga ttgcggaacc
    58981 ggttatgtct acgatgtgat tgccgatttc gacggcaagc cttccattac gctgaccgaa
    59041 tttgcagaac aatgccgtta cgaagaagac attgcacaac tgcgccagtt actgaaacag
    59101 ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc gcagaatatc
    59161 ctctgtcacc gcatcagtga gtcagaggtt atccccgtcg tctgcgacaa cattggtgaa
    59221 agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa gcaagagcgg
    59281 ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa agatctgcaa
    59341 cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatcaag ctggtgttgt
    59401 tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc ctgacggcta
    59461 cgcacatagc ccgatggcgc ggagactttg aaacgaagtg gattaggtaa tacagctgcc
    59521 agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata acgttgtgcc
    59581 gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta aaccgtcagg
    59641 atacgctttt tgctccagac tgtttctatc cccagcgtta atccggcttc cagccctttt
    59701 cgaacccagc tacgcccatc ccataaaaag agatttttgg ctgtctgctg agaaatcgtt
    59761 gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttg ctcaatggaa
    59821 gcgacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat caccgccagt
    59881 cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac gtaacgaaaa
    59941 ttgccatgca gccaggcgct gacttgccgc tcgaccatta ccgctgagaa aggaacaggc
    60001 gcaacgctaa acaacgcgat gcccccgccc cagaaaatgg cgaggacaac cattaaccgc
    60061 agaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat tcagccagaa
    60121 ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg ttctgcgcca
    60181 gcatatatgc cggggtggtg acaatcttat tgtcttcatc aaccacgata tcatcgacag
    60241 gacacggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg atatcagtac
    60301 cgatggtcaa acgtagcggg aaatcgaaaa ttttcggcag tatcgccggt gcaatacaca
    60361 taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt aattcacggt
    60421 caacggtgca ttcgctacca agactggcaa aattgcttaa attcttcgcc gcgccaaaac
    60481 cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga ggacggattt
    60541 caccgcgcgt tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc atcgcttcac
    60601 cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcaa accgcctgcg
    60661 caccgctgcg tgaaatagct aacaacgtca ataccgcttc atgaatttca gaaccgtcat
    60721 agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcttc ctttcgcaat
    60781 tgactgaaac acattaattt ttttaataaa aatgctacgc atcacacatt taactgattc
    60841 atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa aacagtgctg
    60901 tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg gtgttggcgc
    60961 agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc cacccacgct
    61021 ttcagtactt ctacgtcgtg acgccactct tctttcatct cttcaatcca ttcaccgacg
    61081 ttatcttccc aggcgggaag gtcaggagac tgaatttgct gacccagctg ttgcagatgg
    61141 cgtaacccca ctgaccccgc cgcaccttta attttatgcc cttcctcaac aatgcctttt
    61201 ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat cattctctca
    61261 aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata ctgttccagc
    61321 atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac cgtactctcc
    61381 tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg aacagaaagc
    61441 ggcttactca gcacatcatc cattccagca ttgaggtact cttgcttgtc tttcagcacg
    61501 ttagcggtta aggccaccag cggcggtaaa tcctcgcgcg gataacgctt cgtcagttca
    61561 cgagagatat ccagcccggt catatctggc aactgaatat ccagcaatac caggtcgtat
    61621 tcgcccggtt taaacatctc cagcgccgcc ttgccggtca tggcgacatc aacgctgtta
    61681 cctaattttt ccagcacaga acgcgcgaca atcacgttca gttcaatgtc ttccaccagc
    61741 agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc aacctcttct
    61801 gctaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg ttcgctggta
    61861 accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag accaataccg
    61921 gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat ggcgaagatt
    61981 ttatccagct catcctgcgg aatgccgata ccagagtctt ccacttcaaa atgcagcata
    62041 tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgagtgaa tttgacggcg
    62101 ttactgatga ggttccacaa gatctgccgt aaacgcgtcc cgtcggtaat gacctgatgc
    62161 ggtaatggca gcgtcggctc cagattaaag cgcaatcctt tttgttgcgc ctgcaatgcg
    62221 gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggctgatt atcaagctgg
    62281 accttgcgcc gttccatctt atccatgtcg ataatatcgt tgaagatatt ccccagcgtg
    62341 acagccgaaa catggatggt tttgagatat ttttcctgct cggcggtgag ttcagtatcc
    62401 agcagaatgc ggctcaggcc gacgataccg ttcagcggtg tacgcaattc gtgactgatg
    62461 gtggagataa acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg ataccgctta
    62521 cgctcggtaa tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac gcggtcgtag
    62581 tacggcactt tacggatttc aaagcaggct ttgcgtccgt cgggataatc cagccactgc
    62641 tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat gactttcgca
    62701 gcggcttctg gtgagtaaac atcagcaggt ttcaggtgaa ccagttgttt ttcgcttttt
    62761 ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc gttacgataa
    62821 aaaaccaggt cgggtgaagc gtcaaggaag gaacgtaaga atgaggattg ctgctcgagc
    62881 tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa ggtttcctgc
    62941 agttctgctt ccgctttttc acgaacggcg atttcctgat ttagctgggc aatattatct
    63001 tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt ttgcaccagt
    63061 cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag aaagtagacc
    63121 gcccacggcg taatcagcaa accaaagaag atagaacgaa taacatcaat gctttcgacc
    63181 tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac gacgagggcc
    63241 agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc aacataatac
    63301 tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct gccccaataa
    63361 tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc ccctcaatgg
    63421 cctacgccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg ccatgttcat
    63481 tcagataacg atccagctcg accattcccg tccatcgatt ttcgcaccac agcggagcaa
    63541 gcagcgtcgg gcgacgggcg ctggcggaaa tacggtggta gataacttcc ggtggcgtat
    63601 ggcgaatcat ttctccggca gtgagcgtgt aatcctccag ttcaattccg ttcaaacgcc
    63661 ccgcttccca ggctttcgcc ataatgctgc ctttcactat atgcagcgga tgcagcttta
    63721 tgccgtccac gccggtttca accactcgct ccagcgtttg caaacattca gcctgccctt
    63781 cgccaggcag gccaacaatt aagtgagagc acaccttcag cccacgctca cgcgccagct
    63841 gcgtcgtgcg ctgatagcag gcaaaatcat gaccgcgatt tatgcgatgc agtgttttgt
    63901 cgtgggcggt ttgtagcccc agctccagcc acacttcgta gccctggtcc ttatattcgc
    63961 aaagcagatc cagcaccgca tccggcacgc agtccgggcg ggtaccaaca cacaaaccga
    64021 caatattggc ctggctcacc gcctgctgat acatcgaacg cagcacctga acttccgcaa
    64081 aggtgctggt atacgcctga aagtaggcca gatagcgttt agcgcggtta actaaattcg
    64141 cctggtgcgc cagttgctcg gcaatggaac gatgctgctg cgcttcatcg gcaaacgagg
    64201 caacattaca gaatgtgcag ccgccacgcc cgatggtacc gtcacggtta gggcagctaa
    64261 aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg agatcaccac
    64321 caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct tgaaaagagg
    64381 ccaaagcctg ccatttttag cccaattcgg cgatgacctg gatcaatcgt cccagcctgc
    64441 ttttatcaac tgcataacca atcaaaatta ccgaaatttc atgcacaatc acataaatca
    64501 ctgttgctta tcttgtgtca gattttttta tcttcttatg gattttaggc aaaaacagta
    64561 gcatgaaacg ttattaccaa ttaaggcagt ataaaatgct ggctttctca tcaattcaag
    64621 gcaggataag ggttaacacg cctttatgac agtcaggaat tggctgtttc tctaacgaca
    64681 ccccttttag ccttaaatag aaaatccact ttaatttcat acaattaata aagaattttg
    64741 cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag ctttcccgat
    64801 aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata tttatgcagt
    64861 aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag ggggcgaatg
    64921 cgaggtgcgc gtatgacacg caaaccccgt cgccacgctc ttactgtgcc cgtgcgcagc
    64981 ggttcggaag tggggttccc gcagagcctg ggggaggttc acgatatgtt gtacgataaa
    65041 tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg cgaacctagc
    65101 cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg tggcgcgatt
    65161 ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc ggatcgtttt
    65221 tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcaa aaaactacgc cgtcgggatg
    65281 ctcttcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt tgaagaagag
    65341 ctgcaacgcg aaacgttgtc gattgtgggc tggcgtgatg tccccactaa cgaaggcgtg
    65401 ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc aaatttttgt gaacgccccg
    65461 gcaggctggc gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg ccgcattgaa
    65521 aagcgtctcg aagccgacaa agacttctac gtctgtagcc tgtcgaatct ggtcaatatt
    65581 tataaaggtc tgtgtatgcc ggcggatctg ccgcgcttct acctggacct cgcggacctg
    65641 cgtctggaat cggccatctg cctgtttcac cagcgctttt ccactaacac cgtaccgcgc
    65701 tggccgctgg cgcagccgtt ccgctacctc gcgcataacg gtgaaatcaa caccatcact
    65761 ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct gatccctgac
    65821 ctgcacgacg ccgcgccgtt cgtcaacgaa accggctctg actccagttc gatggataac
    65881 atgctggaac tcctgctggc aggtgggatg gatatcatcc gtgccatgcg tctgttagtg
    65941 ccacctgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt ctttgacttt
    66001 aactccatgc atatggagcc gtgggatggc ccggcaggca tcgtgatgtc tgacggtcgt
    66061 tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt catcaccaaa
    66121 gataagctca ttacctgcgc ctctgaagtc ggtatctggg attatcagcc tgacgaagtg
    66181 gtcgaaaaag gccgcgtggg gccaggcgaa ctgatggtta tcgacacccg cagtgggcgt
    66241 attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccaccctta taaagagtgg
    66301 atggagaaaa acgtccgccg tctggtaccg tttgaagatc tgcccgatga agaagtgggt
    66361 agccgcgaac tggacgacga cacgcttgcc agctaccaga aacaatttaa ctacagcgcg
    66421 gaagagctgg attcggtgat ccgcgtactg ggtgaaaacg gtcaggaagc ggtcggttcg
    66481 atgggcgacg ataccccatt cgccgtgctc tccagccagc cgcgcattat ttacgactac
    66541 ttccgccagc agtttgctca ggtaaccaac ccgccaatcg acccgctgcg cgaagcgcat
    66601 gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttctgtga agcagaaggc
    66661 caggcgcacc gtttaagctt taaatcgccg attctgctct attccgattt caaacagctc
    66721 acgacgatga aagaggatca ctaccgcgca gatacgctgg atatcacctt tgacgtcact
    66781 aaaaccacgc tcgaagcgac ggtgaaagaa ctgtgtgaca aagccgaaaa aatggtacgt
    66841 agcggcaccg tgctgctggt gctctccgac cggaatatcg ctaaagatcg cctgccggtt
    66901 ccggctccga tggcggttgg cgcgatccag acccgtctgg tcgagcaaag cctgcgttgc
    66961 gatgccaaca tcatcgtaga aaccgccagc gcccgtgatc cgcaccactt cgccgtgttg
    67021 ctgggtttcg gcgcgacggc tatttatcca taccttgcct atgaaacgct gggccgcctg
    67081 gtagacaccc acgcgattgc caaagattat cgtaccgtga tgctcaacta tcgtaacggc
    67141 atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat cgcctcttac
    67201 cgctgctcga aactgtttga agcggtcggt ctgcacgatg atgtcgtggg cctgtgcttc
    67261 cagggggcag ttagccgcat tgggggggcg agctttgaag acttccagca ggatctgctg
    67321 aatctgtcga aacgagcctg gctggcgcgt aagcctatca gccagggcgg cctgctgaaa
    67381 tatgtacacg gtggcgaata ccacgcctac aacccggacg tggtgcgcac gctgcaacag
    67441 gcggtacaga gcggcgagta tagcgactat caggaatacg cgaagctggt taatgagcgt
    67501 ccggcaacca cgctacgcga tctgctggca attacgccgg gtgaaaacgc ggtcaacatt
    67561 gctgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgccgc gatgtctatc
    67621 ggtgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaacag catcggcggt
    67681 aactcaaact ccggcgaagg cggtgaagac ccggcgcgct atggcactaa caaagtgtcg
    67741 cgcatcaagc aggtagcgtc cggtcgcttt ggggttactc cggcgtatct ggtcaatgcc
    67801 gacgtcattc agataaaagt cgcccagggc gcgaagccgg gtgaaggtgg tcagttaccg
    67861 ggtgataaag tcactcctta cattgccaaa ctgcgctatt cggtgccggg cgtgacgctg
    67921 atctccccgc cgccgcacca cgatatctac tctattgagg atttagcgca gctgattttc
    67981 gacctcaagc aggtcaatcc aaaagcgatg atctccgtga agctggtttc cgaaccgggc
    68041 gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac catcgcaggc
    68101 tatgacggcg gcaccggcgc aagtccgctt tcatcggtga aatacgcagg ctgtccgtgg
    68161 gagctggggc ttgttgaaac ccaacaggcg ctggttgcca acggcttgcg tcacaagatc
    68221 cgtttgcagg tcgatggcgg cctgaaaacg ggtgtcgata tcatcaaagc ggcgattctt
    68281 ggcgcggaaa gttttggctt cggcactggc ccgatggtag cgcttggctg taaatatctg
    68341 cgaatttgcc acctgaacaa ctgcgccacg ggtgtagcaa ctcaggatga caaactgcgt
    68401 aagaatcact atcacggcct gccgttcaaa gtgactaatt actttgagtt tatcgcccgt
    68461 gaaacccgcg agctgatggc acagctgggg gtaacgcgtc tggtggatct gattggtcgc
    68521 accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact ggcactgtcg
    68581 aagctgctgg agactgccga gccgcatcct ggtaaagcgc tctactgcac cgaaaacaac
    68641 ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcaaa accgtttgtc
    68701 gatgaacgcc agagcaaaac cttctggttc gatatccgca acaccgaccg ttctgtcggc
    68761 gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc agccgatcct
    68821 atcaaagcgt acttcaacgg cactgcaggc cagagcttcg gcgtgtggaa cgcgggcggc
    68881 gtggaactgt acctgaccgg tgatgccaac gactatgttg gtaaaggcat ggcgggcggc
    68941 ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc gagcattatc
    69001 ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg ccgcgcgggt
    69061 gaacgtttcg gcgtgcgtaa ctccggtgct atcaccgtgg tagaaggcat tggcgacaac
    69121 ggctgtgaat atatgacggg tggtatcgtc tgtattctgg gtaaaaccgg cgttaacttc
    69181 ggcgcgggca tgactggcgg cttcgcttac gttctcgatg aaagcggcga tttccgcaaa
    69241 cgcgttaacc cggagctggt cgaggtctta agcgttgacg atctggcgat ccatgaagag
    69301 catctgcgtg gtcttatcac cgagcatgtt cagcataccg gttcccagcg tggtgaagag
    69361 attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc gaagtccagt
    69421 gatgtaaaag cactgctggg tcaccgtagt cgtagcgcag cagagttgcg cgtgcaggcg
    69481 cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct gcagcgcgtt
    69541 gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat ttacgagccg
    69601 ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg caacccatac
    69661 tgcgagtgga aatgcccggt acacaactac atcccaaact ggctgaagct cgccaatgag
    69721 gggcgtattt ttgaagcggc ggaactgtcg caccagacaa acaccctgcc ggaagtttgc
    69781 ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct gcaccctgaa cgatgagttc
    69841 ggtgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt cgagatgggc
    69901 tggcgtccgg atatgtccgg cgtgaaacag accggtaaaa aagtggcgat tatcggcgca
    69961 ggtccggcgg gtctggcgtg cgcggatgtc ctgacgcgca acggcgtaaa agcgatcgtc
    70021 ttcgaccgtc acccagaaat tggcggcttg ctgaccttcg gtattccggc tttcaagctg
    70081 gaaaaagagg taatgacgcg ccgccgtgaa atcttcaccg gcatgggtat tgaattcaaa
    70141 ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga ttacgatgcc
    70201 gtgttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa cgaagacgcc
    70261 gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt aatgggcttt
    70321 ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt ggtccttggc
    70381 ggtggcgaca ctgcgatgga ctgcgtgcgc acgtctgtac gccagggggc aaagcacgtt
    70441 acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga agtgaaaaac
    70501 gcgcgggaag aaggtgttga gttcaaattc aacgtccagc cgctgggtat agaagtgaat
    70561 ggcaacggca aagtcagcgg cgtaaaaatg gtgcgcacag aaatgggcga accggatgcc
    70621 aaaggccgtc gccgcgcgga gattgtggca ggttccgaac atatcgttcc ggcagatgcg
    70681 gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa acacagcgtc
    70741 gagctggatt cgcaggggcg catcatcgcc ccggaaggca gcgacaacgc tttccagacc
    70801 agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct ggtcgttacc
    70861 gccatcgccg aaggtcgcaa agcggcagac ggcattatga actggctgga agtttaagcg
    70921 aggtaacaat gaattcgcca gacagtgtct ggcgaattta taaaacgagc tatccaacta
    70981 aattttaagc cattaaatca gccttaacct tcactaccac ctttttaatc tcaccaggtt
    71041 cgccaacaac gcatcccggt ttatgcggtt ctcctggcat aaacacggcg aacattcccg
    71101 gtttcaggat gatggcttgc tcgttttcaa tggcgctgca aagctgataa tcatcttcat
    71161 ggtggaactc ttcacactga cgcgcagtgc ctgccatgcc aaacagaatc cgttcctcac
    71221 cgtttaataa cagctggata tcaatgtatt gttcgtgcaa ttcggctttt ttctcaacgg
    71281 gcgattgagt attaaatgtc atgacattca taaagatatt gtcgccctgt aattcgtaac
    71341 ggccaggcgc tttttcttgc ggtctggcag ctaatgccaa cgttaacgcg tcctgtaacg
    71401 caggatgtaa tccggcagac ggtaatgact gtacttcacc catcatcata atatttctcc
    71461 ctgggccaac agcgcagccc caagtaaacc tgcatcgtgg cggtagtgcg ccgccagtaa
    71521 atcaacatga aatgccgctg gctcctgcgc cagatacgtt tccaccagcg ccagataccc
    71581 ttctgccaga ccaacgctgc caccgaccac cacgcactgg caatcagttg tggctttaat
    71641 atcagcgatc agccttgcaa gcgtacgtgc ggagcggtga atcagttgct gcgcctgctc
    71701 atcgccctgc ccggcgcgcg tgaaaatagt tttcgcattc gcaccagcca actccccctg
    71761 cgctgcagct gcaatgccgc gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca
    71821 gccgcagact ggaccatgcg gatcggcaag cgtatgcccg atatgccccg ccagaccgcc
    71881 agggccggta cgcagtttac cgccgctcac tacaccgccg ccaacgccgg tggaaacagt
    71941 gataaacacc atatcggtta tatcgccatc cagcgcctga tactccgccc atgctgcggc
    72001 ctgcgcatca ttaatggcaa tggtcggcaa attggtaagt tgctccagcg ttttgactaa
    72061 cggaaagtgt aacaatccac cgagattatg cggattcagc gccagcaagc tgccgtcgcg
    72121 aataattccg gttgaagcga tagcaacccg ctgcgcatgt gcttgcaacg gagagactaa
    72181 tgcggataag gcatcacgca gcgcctgcgg agtctggctg gcaggcgtag gaagttctcg
    72241 gcgatcgcgg atctgcccgt cagcgccaat cagcgcggcg gcaagtttag taccgccgat
    72301 atcaatcgcc agtgtggtca tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa
    72361 atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc
    72421 gcatccgccg cctgagcagg cgtgttgtaa cgcccttcgg caatcacccg gcatccggcg
    72481 tcactcaacg ttttcaccag cgccagatcc ggctcttctg gcgtttcagg cgtggtatag
    72541 ccagaaagcg tagtgccaat aatttcggct cccagctttt ggcatgccag gccgtcttcc
    72601 ggcgttgagc agtcggtcat cgccagtaaa ccgtgatggt gaatacgtgc cagcagcgtt
    72661 tcaacaggca ccggacgcgg tcggtcagtg ccgtcaatgg cgataatgtc cgcgcccgcc
    72721 tgcgccagcg catcaacatc ttcaatatag gccgtgatgc gtaccggaga atcctccaga
    72781 tcgcgcttca caatcccaat aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt
    72841 gccacacctt caatacgaat ggcaaccgcg cccgcctgct ctgccgctaa tgccatggca
    72901 gcgacgattt cgggtttatc gagcgggctg tccggaaccg gctggcagga gacaatcagg
    72961 ccaccgttag cagcgatttt ttgatccagt tgtgcaagta acgacataca tcttccctta
    73021 gcgaaaggcc cggtacagag accgggcaac aggattaact tttggttttg actaaatcgt
    73081 ttttggcgct gccaaatggc acggcaccgc taaatggttt gccgtcgata gcgtcatgag
    73141 tacgcaatgc ttccgggcgc aaccaacgct gaacacgaga aggcatatcc agaccaatca
    73201 gcaggatcac cacaaacgtt aaactaaacg agagcgagcc aagtgccgtt cccagatcca
    73261 gacgttgagc gatcaacgcg ccgaggattg gagctagtgc accgcccagt gcgccaacgt
    73321 tgtaggtaaa gcccagcccc gccgcacgct ggtcggtatc gaaataaccg ccgatcagtt
    73381 tcggtaaaat ccctgagatg ccctgaccaa gcatttgctg gaagaacagt aacaaaccga
    73441 gcacccagac gtttgcgccg ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca
    73501 gcagactaca aacgtacgct ttgcgggttc ccagccagtc accgaggaag ccaccgacgc
    73561 agcaccccac cgccgcgcca aagccactaa agaacagcac attggcgaca gtatgcgggt
    73621 cataagccag atcggttttc agatacgttg gcagcagcgc ctgaatcggc catgagtaga
    73681 ggaaagcgaa caacacgacc accatcagca ttacgcccgt tggccagcgt ttgcccgtac
    73741 tctgcaccat aaagctgata aagattgcgg cgcataacag cccgagtaca gcgacgattg
    73801 cggcattttg caggttaccg gcgaagcaga accacagcgc agtagccgcc gccagcgtca
    73861 ttacgatatt ggcaatgcga tgttcaccac ggtaaagaat gtctaccatc gtgcgtactg
    73921 gtgctttacc cccgtgtttc tctttccagt cttccgcttc cgggatgttt ttacgcagcc
    73981 agagagcaaa gatgattggc aaaatgccga taaagaacag cgcacgccag ccccagaccg
    74041 gaaccaccag actatagacc tgagcggcaa cgacggcccc cactgagaag cctgaaatca
    74101 gaaaaccact ggctttgtta cgcagatgct ttggccagct ttcaatgaca taggtggcgc
    74161 tggaaccgta ttcacccgcc atccccatgc cgatgaccag acgagcgata aacatggtga
    74221 tgtagcctgg cgcaaagccg caggccagcg tcccggccga gaagagaacg atgctggtga
    74281 ccattgccag acgacgcccg tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc
    74341 agcgagagat aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc agcccgaatt
    74401 caccctgtac ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg tcaagcagat
    74461 atcccaacca ggcagcggaa aatgcccgcc attgtgcgcg gttaagatgg cgataccacg
    74521 ggatattctg ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg cccacacgct
    74581 ttggtatgaa aattgtaggg tacagatgcg tttatttccc ctcacccggt aggggcgagc
    74641 gaggggaaac aactcacccg cgctcttgca tcaactgctg agccagcgcc ttcagttctg
    74701 gcagatattt ttcatctacc ggaccaaacg gtttgcggca cagcggcaca gaaacgacat
    74761 ccatataatg caggacagtt ttcagcccac ggaatacgcc agttttgatc agtaaatcaa
    74821 tgactttgtt gcactcagtt tgcagtttct gcgcggtctg gatatcgcct tctttcagcg
    74881 ccttcacgat cccctgataa cgccagccca tgatgttgta agtgctgccg ataccgccgt
    74941 ctgcgcccgc cagaagacca gaggcgaaga tttcgtcgta accgttatag agcaccagat
    75001 caggatgttc acgacggatc tgctccatct gatagagatc gccagaggtc tgtttcagcg
    75061 cacctacgcc aggcagcgta accaaggtgt tgatctgatc cagggtcagt tttaccccgc
    75121 tcagggctgg aatgttgtac accaccatcg gcaacccatc cgctgaatca ataattgccc
    75181 gatagtgatc gcagtgttct tcaaagctga aaggatagta gaacggcgtg acggcggaga
    75241 cggcatcgaa gccataacgt ttagccgatg ccgcaagttg ttggctttcg gcggtgctga
    75301 cgcaaccgac atgggcgatg agtttaatct tacctttcgc ctcttcagcg acgatttcca
    75361 gtacctgttc gcgctcggaa aggctttgta caaaggcttc gccggtcgaa ccacccacgt
    75421 acaaaccgtc gatgccctgc tgaatattga actgaaccag gcggcgcaga ctcgctttat
    75481 ccagtgcttg ttgttggtca aaaggagtca ggagtgcagc cattacgcca cgtaaattcg
    75541 ttgccataaa tacctctgaa gtgatgcttg tctgataaac gatatacctt tatacctgtt
    75601 ataccagatc aattaagcaa caccccatac agaaagctta taatgcgatc tgcttcacta
    75661 aagtggcatt atttcttttt gttggtggtc tgaccgaagg cgtgccaggt agcagagacg
    75721 ctgttgagat gcgattgcaa cgcgcgatcg gcttcgtcag gatcatgacg gcggatcgcg
    75781 tcaacgatcg caatatgctg ttgatagcta acgttgttat gctcatgcag agcctgatcg
    75841 gtcacggttg gacgtgcggc gattagccag tcgagcaggg caacgtggat cgccatgaag
    75901 attgggttac ccgggatctc cgccagaacg cggtggaaat caacgtctga gcgaatgaat
    75961 gccgcgttgt tatccagcga ctgactgttg atttccagtg cttttgccag caaatcgatt
    76021 tgctcatcgg tggcgtgttc agccgcatag cgcaccaggc tggattcaaa gaacagacgt
    76081 aattgttcga aatgggcaat cccaccagga tgagaaagga aatctttcgc catgccggaa
    76141 agctcgccga tgatggtgtc cgcagaagga cgtgagacgc gggcacgttc gccgttgttt
    76201 atttgcacca gacctttgcg ttttaacgct gccagcgctt cgcgcaccga aggacgcccg
    76261 acgttaaaga acgccatcag ttcgcgttca gacggtaatt gttcaccttc gccaaattca
    76321 cgacggcgga tcatctgttc cagctcttct tccaccattt cggagagttt tttacgcgcc
    76381 agcgggcggc tgcgcaagtt gcgcccgata gtggtagtag aatcttcagc ttgcggatca
    76441 aatgcgttca taggactcat tcaggaaaat gcgttagcgg ataataacga cgctatcgta
    76501 gcacagtatc aaagtcaggc gaaaagatga cagatagcaa aacaggccac atgggcctgt
    76561 tttcatttta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    76621 catcgtcagg atgagtttca tcatcgtgat ctggcttgtc gccatcgata accgacataa
    76681 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggcatct tcatcgtagg
    76741 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    76801 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    76861 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    76921 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    76981 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    77041 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc
    77101 tgcttcagtt aaagaagcaa ggaaagagtc acgttcaaag acgcgggtca tatagccttt
    77161 cagctctttc gcacccgggc cgctgaactc gatgcccagt tgcggcagac gccacagcag
    77221 aggagcaagg tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    77281 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catcggcttc
    77341 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    77401 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    77461 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    77521 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    77581 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    77641 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    77701 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    77761 ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa atcaaacaaa
    77821 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    77881 cctttgtggt gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    77941 ttgacttagt cccttattgg cgatgtggtt tttgttttac ctgtctgtca ggtggcagca
    78001 aaaagcaact ttccattttt tacgctgatg cagattttag ccataaaaaa acccgccgaa
    78061 gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    78121 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    78181 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    78241 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    78301 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    78361 tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    78421 cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca gtgccgtagt
    78481 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    78541 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    78601 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    78661 caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg tggtgatagt
    78721 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    78781 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac
    78841 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    78901 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    78961 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    79021 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagtttcgg gaaaaaaaac
    79081 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
    79141 ttgtgatttg tggcaggttt taccccgcca aatgctcgcg cttcaaatac tcttcgcttt
    79201 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccgc tcgccctgat
    79261 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
    79321 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
    79381 catcaaacaa catgaccgtg tgaaagaggc gtgagagcgc aatatagtca tgctgactgc
    79441 gggcgtcgac gcacagcgta gtaaaagaaa ccgccagcgt ctggttctcg acgcccattg
    79501 tcgccaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc gcccccgcca
    79561 gcgccaacca tagtttatcc atttgcgccc gggtttcatc gttgaggggc gaaagccaca
    79621 gatgcgcctg agtgagtgta cgcaggcgat aatcaacacc agcgtccacg ttcattacat
    79681 cacaatgctg tttaatggca tcgattgcag gcaaaaaacg cgcacgttgc aggccatttc
    79741 gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca
    79801 gggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt
    79861 caaaacagag cacgtcagtt tctgctttaa agcgatcggc aataatttcc agcggatcgg
    79921 tctgcccctg caaagcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca
    79981 ggcgctgttt ccgctctccc ggcagacttt gatagaaaag gtccatcagc caggttttcc
    80041 cgcgtcctac accgccccac atatataagc cacgcactgg catatgcttt gtgtcttcgc
    80101 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg
    80161 tgctattgac gagttcctta taaataattt ccaggcggct gacggcctct ttttgaacgt
    80221 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
    80281 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
    80341 aaaaaaggcc gttctacact acgcgatatg ctgtcgggat tccacttctg tggaattaac
    80401 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
    80461 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
    80521 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
    80581 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
    80641 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
    80701 atcagcacat ggcgaaaagc tccagtagcc tgctgccgga actgtctgcg gaagcaaacc
    80761 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
    80821 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
    80881 ttatttttgg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
    80941 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
    81001 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag ctgttgagtg
    81061 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
    81121 caggccaggt tgccgatcag gcccccctcc ccagtctggc tccaatgctg gaaaaagtgc
    81181 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
    81241 aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa cccttcgaag
    81301 gtttaggctc cggtgtcatc atcaacgcca ataaaggcta tgtgctgacc aacaaccatg
    81361 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
    81421 aactgattgg tagcgatgac cagagcgata tcgccctgtt gcaaattcaa aacccgagca
    81481 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtgg
    81541 cggtcggtaa cccatttggc cttgggcaaa ccgctacctc cgggattata tctgcgttag
    81601 gccgcagcgg tctgaatctc gaaggtctgg aaaactttat tcaaactgat gcctccatta
    81661 accgcggcaa ttccggtggt gcgctgttaa accttaacgg tgagttaatt ggcatcaaca
    81721 ctgcaatcct tgcgcctggc ggcggtagcg tcgggattgg atttgccatt cccagtaata
    81781 tggcgcgaac actggcgcag cagcttatcg actttggaga aatcaaacgc ggcctgttag
    81841 gtatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
    81901 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag
    81961 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
    82021 ctcgtatcgc gaccaccgag ccgggcacga aagtgaagct tggcctgctg cgtaacggca
    82081 aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa
    82141 tgatcacgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggtggca
    82201 aaggtattaa gatcgatgag gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
    82261 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
    82321 aagtgctggc ggcaaaaccg gccataatcg ccttgcaaat catacgcggc aatgaaagcc
    82381 tctacctact aatgcgttaa tgtcgtaaac cgggcatcag gctgacgtgt gatgtccggt
    82441 taactcgtgg tatgctgctg ccgttccctt ttttaacgat gcctccatca tgtttgtgaa
    82501 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
    82561 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgaaacgcc
    82621 tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttataaccg
    82681 cggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ctggtgtaat
    82741 catggatcaa cgcgggtata tcatcaccaa taaacacgtg atcaacgacg ccgatcagat
    82801 catcgtcgcc ttacaggatg gacgtgtttt tgaagcgtta ctggttggct ctgactctct
    82861 aaccgatctg gcggtgctga aaattaacgc cacaggcggt ttaccaacga ttccgatcaa
    82921 tccacgccgc gtaccgcaca ttggcgatgt ggttctggcg atcggtaacc cgtacaacct
    82981 cgggcagacc attacccagg ggattatcag tgccacgggt cgaatcggtc tgaacccgac
    83041 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
    83101 gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa
    83161 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag cgaccaaaat
    83221 tatggataaa ctgatccgtg atggccgtgt gatccgtggc tacattggca ttggtggacg
    83281 tgagatcgcc ccattacatg cgcagagtgg cggcatggat caactgcaag ggatcgtggt
    83341 taacgaagtg tcgcctgatg gcccagcggc aaatgcaggc atccaggtca acgatctgat
    83401 tatctctgtg aataacaaac ccgctatttc cgccctggag acaatggatc aggtcgcgga
    83461 aattcgccct ggttcggtga tcccggttgt cgtgatgcgt gacgataaac agttaacatt
    83521 gcaggtcacc attcaggaat atccggcgac caactaaggt cagcgtaaaa aaacaaaaac
    83581 cggagtctgt gctccggttt tttattatcc gctaatcaat tacttattaa cgaactcttc
    83641 gcccagggcg atatctttct tcagcgtatc cagcatacct tccagcgcgt tctgttcaaa
    83701 tgcgctcaga gtaccgatag atttacgctc ttccacgccg tttttaccca gcagcagcgg
    83761 ttgagagaag aaacgagcgt actgaccgtc gccttcaacg taggcacatt cgacaacgcc
    83821 ttgttcgccc tgcagtgcgc gaaccagaga cagaccaaaa cgtgcagctg cctggcccat
    83881 agacagggtt gcagacccgc caccggcttt cgcttcaacc acctcagtac ccgcgttctg
    83941 gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga
    84001 cagcagcggc agaatggtaa caccagagtg accaccaata accggcactt caacttcgcc
    84061 tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat ccagcgtggt
    84121 aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt cagcagcaat
    84181 cgcaactgta gtgttaaccg ggttagtgat aataccaatg cacgctttcg ggcaggtttt
    84241 cgcaacttgc tgtaccaggt ttttcacgat accggcgtta acgttaaaca ggtcggaacg
    84301 atccataccc ggtttacgcg ctacgcctgc agagataaga acgacatctg caccttccag
    84361 cgccggagtc gcatcttcac ctgaaaaacc tttgattttc acagcagtag ggatatggct
    84421 cagatcgaca gccacaccgg gagtcactgg agcgatatca tacagagaga gttctgaacc
    84481 tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc
    84541 gaggactgcg actttcatcc taaactcctt attatattga taaactaaga tatgttgctc
    84601 cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct caagaatgtg
    84661 tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc taacttacgt
    84721 aattaacagt cacttatgca ttagcgcaac attccaacag gtggtgacaa tataccctac
    84781 cgctcagcca aaacaatatc aatttgataa caattaattt acttttaagc agaatttgca
    84841 ggccgtgacg caggcatgtt tctcaataac gaaatttgat aaaatcccgc tctttcataa
    84901 cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg tatgcacaat
    84961 aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag caagaagaac
    85021 tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag ggcgaaatcg
    85081 tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc tcgcggatgt
    85141 tgaccaagtt tggtgcggta cgtacacgca atgccaaaat ggaaatggtt tactgcctgc
    85201 cagctgaact gggtgtacca accacctcca gtccattgaa gaatctggta ctggatatcg
    85261 actacaacga tgcagttgtc gtgattcata ccagccctgg tgcggcgcag ttaattgctc
    85321 gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct ggcgatgaca
    85381 ccatctttac tacccctgct aacggtttca ccgtcaaaga gctgtacgaa gcgattttag
    85441 agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt
    85501 gcttttgcct atcaacccac tgctttcccc tgcgattcat ttaacgaata gtgcgtttta
    85561 ctgcgacata tcatt
//
LOCUS       DACADA010000003        76898 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.003, whole genome shotgun sequence.
ACCESSION   DACADA010000003 DACADA010000000
VERSION     DACADA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.003"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(404..1726)
                     /gene="hipA"
                     /locus_tag="ISB17_000172"
     CDS             complement(404..1726)
                     /gene="hipA"
                     /locus_tag="ISB17_000172"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HAP1978161.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK"
     gene            complement(1726..1992)
                     /gene="hipB"
                     /locus_tag="ISB17_000173"
     CDS             complement(1726..1992)
                     /gene="hipB"
                     /locus_tag="ISB17_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HAP1978162.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            2300..3058
                     /gene="tam"
                     /locus_tag="ISB17_000174"
     CDS             2300..3058
                     /gene="tam"
                     /locus_tag="ISB17_000174"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAP1978163.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3062..3976)
                     /locus_tag="ISB17_000175"
     CDS             complement(3062..3976)
                     /locus_tag="ISB17_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978164.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4173..5624)
                     /locus_tag="ISB17_000176"
     CDS             complement(4173..5624)
                     /locus_tag="ISB17_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP1978165.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5851..7269)
                     /locus_tag="ISB17_000177"
     CDS             complement(5851..7269)
                     /locus_tag="ISB17_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1978166.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7408..7767)
                     /locus_tag="ISB17_000178"
     CDS             complement(7408..7767)
                     /locus_tag="ISB17_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAP1978167.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(7767..8693)
                     /gene="glsB"
                     /locus_tag="ISB17_000179"
     CDS             complement(7767..8693)
                     /gene="glsB"
                     /locus_tag="ISB17_000179"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAP1978168.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8757..10145)
                     /gene="sad"
                     /locus_tag="ISB17_000180"
     CDS             complement(8757..10145)
                     /gene="sad"
                     /locus_tag="ISB17_000180"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAP1978169.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10246..11127
                     /locus_tag="ISB17_000181"
     CDS             10246..11127
                     /locus_tag="ISB17_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978170.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            11205..11288
                     /gene="yneK"
                     /locus_tag="ISB17_000182"
                     /pseudo
     CDS             11205..11288
                     /gene="yneK"
                     /locus_tag="ISB17_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11466..12656
                     /gene="ydeA"
                     /locus_tag="ISB17_000183"
     CDS             11466..12656
                     /gene="ydeA"
                     /locus_tag="ISB17_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAP1978171.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12681..13346)
                     /gene="marC"
                     /locus_tag="ISB17_000184"
     CDS             complement(12681..13346)
                     /gene="marC"
                     /locus_tag="ISB17_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAP1978172.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13558..13992
                     /gene="marR"
                     /locus_tag="ISB17_000185"
     CDS             13558..13992
                     /gene="marR"
                     /locus_tag="ISB17_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAP1978173.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            14013..14396
                     /gene="marA"
                     /locus_tag="ISB17_000186"
     CDS             14013..14396
                     /gene="marA"
                     /locus_tag="ISB17_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAP1978174.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14428..14646
                     /gene="marB"
                     /locus_tag="ISB17_000187"
     CDS             14428..14646
                     /gene="marB"
                     /locus_tag="ISB17_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAP1978175.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14677..15576)
                     /gene="eamA"
                     /locus_tag="ISB17_000188"
     CDS             complement(14677..15576)
                     /gene="eamA"
                     /locus_tag="ISB17_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAP1978176.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            15771..16958
                     /gene="ydeE"
                     /locus_tag="ISB17_000189"
     CDS             15771..16958
                     /gene="ydeE"
                     /locus_tag="ISB17_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAP1978177.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            17085..17180
                     /gene="mgtS"
                     /locus_tag="ISB17_000190"
     CDS             17085..17180
                     /gene="mgtS"
                     /locus_tag="ISB17_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAP1978178.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17399..18289)
                     /gene="dgcZ"
                     /locus_tag="ISB17_000191"
     CDS             complement(17399..18289)
                     /gene="dgcZ"
                     /locus_tag="ISB17_000191"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAP1978179.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(18543..18647)
                     /locus_tag="ISB17_000192"
                     /pseudo
     CDS             complement(18543..18647)
                     /locus_tag="ISB17_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
     gene            complement(18796..20841)
                     /gene="dcp"
                     /locus_tag="ISB17_000193"
     CDS             complement(18796..20841)
                     /gene="dcp"
                     /locus_tag="ISB17_000193"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAP1978180.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            20978..21724
                     /gene="ydfG"
                     /locus_tag="ISB17_000194"
     CDS             20978..21724
                     /gene="ydfG"
                     /locus_tag="ISB17_000194"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAP1978181.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            21813..22499
                     /locus_tag="ISB17_000195"
     CDS             21813..22499
                     /locus_tag="ISB17_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1978182.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            22676..22879
                     /gene="ydfZ"
                     /locus_tag="ISB17_000196"
     CDS             22676..22879
                     /gene="ydfZ"
                     /locus_tag="ISB17_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAP1978183.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(22915..24375)
                     /locus_tag="ISB17_000197"
     CDS             complement(22915..24375)
                     /locus_tag="ISB17_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1978184.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(24464..25831)
                     /locus_tag="ISB17_000198"
     CDS             complement(24464..25831)
                     /locus_tag="ISB17_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1978185.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALQDAAEDQPHVVRAAQ
                     PSRSL"
     gene            complement(25889..26908)
                     /locus_tag="ISB17_000199"
     CDS             complement(25889..26908)
                     /locus_tag="ISB17_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HAP1978186.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(26920..28134)
                     /gene="rspA"
                     /locus_tag="ISB17_000200"
     CDS             complement(26920..28134)
                     /gene="rspA"
                     /locus_tag="ISB17_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAP1978187.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(28340..28666)
                     /locus_tag="ISB17_000201"
     CDS             complement(28340..28666)
                     /locus_tag="ISB17_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAP1978188.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            28801..29142
                     /locus_tag="ISB17_000202"
     CDS             28801..29142
                     /locus_tag="ISB17_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAP1978189.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            29177..29737
                     /gene="speG"
                     /locus_tag="ISB17_000203"
     CDS             29177..29737
                     /gene="speG"
                     /locus_tag="ISB17_000203"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAP1978190.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(29740..30450)
                     /locus_tag="ISB17_000204"
     CDS             complement(29740..30450)
                     /locus_tag="ISB17_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAP1978191.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            30558..30863
                     /locus_tag="ISB17_000205"
     CDS             30558..30863
                     /locus_tag="ISB17_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAP1978192.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            31062..33488
                     /locus_tag="ISB17_000206"
     CDS             31062..33488
                     /locus_tag="ISB17_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAP1978193.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDKYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            33549..34262
                     /locus_tag="ISB17_000207"
                     /pseudo
     CDS             33549..34262
                     /locus_tag="ISB17_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            34307..35118
                     /locus_tag="ISB17_000208"
                     /pseudo
     CDS             34307..35118
                     /locus_tag="ISB17_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            35129..35746
                     /gene="dmsB"
                     /locus_tag="ISB17_000209"
     CDS             35129..35746
                     /gene="dmsB"
                     /locus_tag="ISB17_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP1978194.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            35748..36602
                     /locus_tag="ISB17_000210"
     CDS             35748..36602
                     /locus_tag="ISB17_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP1978195.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            36645..37259
                     /gene="dmsD"
                     /locus_tag="ISB17_000211"
     CDS             36645..37259
                     /gene="dmsD"
                     /locus_tag="ISB17_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAP1978196.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            37420..38712
                     /gene="clcB"
                     /locus_tag="ISB17_000212"
     CDS             37420..38712
                     /gene="clcB"
                     /locus_tag="ISB17_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAP1978197.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(38665..39360)
                     /gene="bioD"
                     /locus_tag="ISB17_000213"
     CDS             complement(38665..39360)
                     /gene="bioD"
                     /locus_tag="ISB17_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP1978198.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(39485..40705)
                     /gene="mlc"
                     /locus_tag="ISB17_000214"
                     /gene_synonym="dgsA"
     CDS             complement(39485..40705)
                     /gene="mlc"
                     /locus_tag="ISB17_000214"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAP1978199.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(40840..41733)
                     /locus_tag="ISB17_000215"
     CDS             complement(40840..41733)
                     /locus_tag="ISB17_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978200.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            41840..43093
                     /locus_tag="ISB17_000216"
     CDS             41840..43093
                     /locus_tag="ISB17_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1978201.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            43490..43825
                     /locus_tag="ISB17_000217"
     CDS             43490..43825
                     /locus_tag="ISB17_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAP1978202.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            43918..44001
                     /locus_tag="ISB17_000218"
     CDS             43918..44001
                     /locus_tag="ISB17_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978203.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            44101..44922
                     /locus_tag="ISB17_000219"
     CDS             44101..44922
                     /locus_tag="ISB17_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP1978204.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(44961..45290)
                     /gene="mdtI"
                     /locus_tag="ISB17_000220"
     CDS             complement(44961..45290)
                     /gene="mdtI"
                     /locus_tag="ISB17_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAP1978205.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(45277..45642)
                     /gene="mdtJ"
                     /locus_tag="ISB17_000221"
     CDS             complement(45277..45642)
                     /gene="mdtJ"
                     /locus_tag="ISB17_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAP1978206.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            46054..47088
                     /gene="tqsA"
                     /locus_tag="ISB17_000222"
     CDS             46054..47088
                     /gene="tqsA"
                     /locus_tag="ISB17_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAP1978207.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(47113..48501)
                     /gene="pntB"
                     /locus_tag="ISB17_000223"
     CDS             complement(47113..48501)
                     /gene="pntB"
                     /locus_tag="ISB17_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAP1978208.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(48512..50044)
                     /gene="pntA"
                     /locus_tag="ISB17_000224"
     CDS             complement(48512..50044)
                     /gene="pntA"
                     /locus_tag="ISB17_000224"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAP1978209.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            50568..51512
                     /locus_tag="ISB17_000225"
     CDS             50568..51512
                     /locus_tag="ISB17_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1978210.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            51698..53080
                     /locus_tag="ISB17_000226"
     CDS             51698..53080
                     /locus_tag="ISB17_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1978211.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            53117..53839
                     /gene="folM"
                     /locus_tag="ISB17_000227"
     CDS             53117..53839
                     /gene="folM"
                     /locus_tag="ISB17_000227"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAP1978212.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(53836..54171)
                     /locus_tag="ISB17_000228"
     CDS             complement(53836..54171)
                     /locus_tag="ISB17_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAP1978213.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            54300..55019
                     /gene="rstA"
                     /locus_tag="ISB17_000229"
     CDS             54300..55019
                     /gene="rstA"
                     /locus_tag="ISB17_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAP1978214.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            55023..56324
                     /gene="rstB"
                     /locus_tag="ISB17_000230"
     CDS             55023..56324
                     /gene="rstB"
                     /locus_tag="ISB17_000230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAP1978215.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            56400..57329
                     /gene="tus"
                     /locus_tag="ISB17_000231"
     CDS             56400..57329
                     /gene="tus"
                     /locus_tag="ISB17_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAP1978216.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(57326..58729)
                     /gene="fumC"
                     /locus_tag="ISB17_000232"
     CDS             complement(57326..58729)
                     /gene="fumC"
                     /locus_tag="ISB17_000232"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAP1978217.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(58872..60518)
                     /locus_tag="ISB17_000233"
     CDS             complement(58872..60518)
                     /locus_tag="ISB17_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAP1978218.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            60717..61892
                     /gene="manA"
                     /locus_tag="ISB17_000234"
     CDS             60717..61892
                     /gene="manA"
                     /locus_tag="ISB17_000234"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP1978219.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            61993..63501
                     /locus_tag="ISB17_000235"
     CDS             61993..63501
                     /locus_tag="ISB17_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAP1978220.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(63546..64812)
                     /gene="uidC"
                     /locus_tag="ISB17_000236"
                     /pseudo
     CDS             complement(63546..64812)
                     /gene="uidC"
                     /locus_tag="ISB17_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(64851..66223)
                     /gene="uidB"
                     /locus_tag="ISB17_000237"
                     /pseudo
     CDS             complement(64851..66223)
                     /gene="uidB"
                     /locus_tag="ISB17_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(66220..68031)
                     /gene="uidA"
                     /locus_tag="ISB17_000238"
     CDS             complement(66220..68031)
                     /gene="uidA"
                     /locus_tag="ISB17_000238"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP1978221.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(68421..69011)
                     /locus_tag="ISB17_000239"
     CDS             complement(68421..69011)
                     /locus_tag="ISB17_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1978222.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(69240..70007)
                     /gene="hdhA"
                     /locus_tag="ISB17_000240"
     CDS             complement(69240..70007)
                     /gene="hdhA"
                     /locus_tag="ISB17_000240"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAP1978223.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGDGVQELN"
     gene            complement(70119..71147)
                     /locus_tag="ISB17_000241"
     CDS             complement(70119..71147)
                     /locus_tag="ISB17_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAP1978224.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            71322..72914
                     /locus_tag="ISB17_000242"
     CDS             71322..72914
                     /locus_tag="ISB17_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAP1978225.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            72924..74096
                     /gene="malY"
                     /locus_tag="ISB17_000243"
     CDS             72924..74096
                     /gene="malY"
                     /locus_tag="ISB17_000243"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAP1978226.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            74200..75201
                     /gene="add"
                     /locus_tag="ISB17_000244"
     CDS             74200..75201
                     /gene="add"
                     /locus_tag="ISB17_000244"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP1978227.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(75237..76277)
                     /locus_tag="ISB17_000245"
     CDS             complement(75237..76277)
                     /locus_tag="ISB17_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1978228.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            76520..76645
                     /gene="blr"
                     /locus_tag="ISB17_000246"
     CDS             76520..76645
                     /gene="blr"
                     /locus_tag="ISB17_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAP1978229.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT        19429 a        19713 c        18301 g        19455 t
ORIGIN      
        1 tagtcactgt tctctgtatt tcacattgca tatcaacacg atatgctgag attagctaac
       61 ttgagattaa agataattac acggcgctat gaattatatt cagaagcgtg agggatatag
      121 tgccatcaaa gcgctggctg ctgtaaacaa cgcaattatc cagctcatgg tctatggtgt
      181 gccatcactg aacagaggtt gatttttcta ccgaaacggg agggaagtca gaaatcacaa
      241 cgtattgatt tatctgtgtg tggatgtgaa tggtgaagta aaggaccttc ataatcaatt
      301 taacataata tacattatgc gcaccaatat aaaccaaggg cgaatccagt cgttggcggt
      361 tcatgattgt tctgctcatt aacaacgacc aaaaccccat atcttactta ataccgtatt
      421 ctcggcttaa ccgatcgtgc aacctcaaca cattggtttc aactgccgtc accacgttct
      481 ccggaaaatc tgtcggtaat gaagtcttca cgttatccag tgctgccgga atcattctgg
      541 caaagtcact caggatttca tgcatctgca cttccgggaa tttcagcacc tttgctgtcg
      601 ccagaaaatg tcgcggataa attttatcga ttgccgtttt tttgcctttg gatgcgttaa
      661 gccccattgc cagtttgaga tcgctgatgt gtattcccgt accgccaagg accggaaatg
      721 ccgaaatgat gtcgtaaaat ggtgtgagcc gataactgcc gccagcctga ataaatacgg
      781 agaagttttt tgcatggcca tccgttgcgc caatcaacca ctggaagacc tgaaacttca
      841 taaaatcata gcgatctttc agcgcctcgc tggaccccat caaaaaagcc ataatttgcg
      901 cgataccggg gcctccatct gattcatatt tcaccgatga aggtaaaccg aatgtctggc
      961 acatatcctc ctgtggcaag cgaagtaaaa ccgttcgctc agtattccaa cgcctgtcaa
     1021 aacgttcgac cgctaacgcg cgcacccttc ccgctttaat gatttctgcg tccggaacat
     1081 tcaacccgag ttctttcgcc agcagcaaac agtaatactc attatcaacg ctttggctaa
     1141 gatcgagcgt cgcattgggc tgtctgattt cgccaatcgg caatttgata atgtgcgtcg
     1201 ttggcgttat tcccttcgga atgcaccagt cattgcctat tctgagcagc gcagtcttct
     1261 cctgtgcgcc agcaaccgag atgcgaaagt cattttcttc tctaatcatg cctaacggga
     1321 tatctgcttt ataagccgtt aatacttctt caagtctggc ttcagtaagc ttttcccatg
     1381 ccattatcgg acacgttacg gtttcatctt cggggattaa cgtcacggca ccaacgctgt
     1441 ctcgccctat ttctgacaat aaatcaaacg gttgtctgga tttagcatga taacgtttaa
     1501 cgatccggtc acgtacaatc gggctatcgg gtaacaggtt atcgaagaag ttaaatacgg
     1561 catcagaggt gatattcccc ctctgcaatg gcagcgaaag tgacaacggt ctggcataac
     1621 ggctggctaa ccactcagct gcatacttaa aggtgtgcgc gccgttggct aacttcgtta
     1681 actcgcctac ccgctggttg ttcatccaag tgactagttt aggcattacc actccagatc
     1741 ttgctgttct gttgcttctg gcgaggcatt tttcgcgtcg catagcgtca ttgagagttc
     1801 aagcgactgt aaaatcttaa aaaatgtcgt gagcgaggta ttgtcaggat tgttttcgaa
     1861 attggaaatt gtcgcctgct taatgccgat ttttttcgcc aactcgctct gcgtccagcc
     1921 gttttgctgg cgaaccagtt tcattgcatt cgccaattgc gttgggctat agatcttctg
     1981 aaagctcatc atgtcatacg tccacagcaa atttatccgc ttaaggggat attatcagtt
     2041 ttatccttta gtgaggataa agtcaatctg gaaacacatc ttatccgctc tacgggataa
     2101 acgctgcgat atccgcgatc gcggataaaa aggaggattt aaaaaagcac atcctaatgc
     2161 ggtggcttct ttgactaccg gaatctgtaa tcttaatgac cgggccgcgt aaaaaacgtc
     2221 tgttcaatgg ggtgatgccg tgaggcgaat ttatcagttt tatctacaat tggggtaacg
     2281 cgctgacggg agtaaaaaaa tgtctgactg gaacccctct ttatatctgc actttgccgc
     2341 tgaacgatcg cgtccggcgg tggagctgct tgccagggtg ccgctggaga atatcgaata
     2401 cattgccgat cttggctgtg gcccaggtaa catcaccgcc cttctacacc aacgttggcc
     2461 tgctgccagg ataacgggca tcgactcatc tccggcgatg attgctgaag cgcgcagtgc
     2521 tttgccagac tgcctgtttg tggaagcaga tatccgcaac tggcaaccgg aacaggcact
     2581 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     2641 tcatttggtt tcgttactta attcgcatgg tgtgttggca atacagatgc cagataactg
     2701 gctagagcca acccatgtgc tcatgcgcga agtcgcctgg gaacaaaatt atccggatcg
     2761 cgggcgtgag ccgctggctg gcgttcatgc ttactacgat attctgagtg aagccggatg
     2821 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     2881 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     2941 gcagcatttt cttacgcgct accatcagat gctggaagag caatatcccc tccaggagaa
     3001 cggacagata ttgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     3061 atcacgtcag ctggtaatgg cgatcgggaa gaattttcgc tggaatttct gcttcatcat
     3121 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     3181 tttcagtacc gaagggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     3241 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcaaacg
     3301 gcaacatgat acaaaacaga tacacggtgc gatgcaaaat cagggtgtag gcaaagggaa
     3361 ttggcgtata ggcaatgcgc tcgcatcctg ccaggaccgc tgaaatatca ttaagccgat
     3421 cgttgaggct aataaacagg atatctgaaa gttgtccatt gcgtcgctga accgccaacc
     3481 actcccccat tattaacaag atgcggttag ctggagagtt agaagccagt acacgctgaa
     3541 gatcttcagt cttgagataa tgagccagca cttctgcctg tggctgtttg cgtaatgtca
     3601 tgcgtaaaca gtgggcgaag acgatttgca gccgggcaaa ctcccttaca cttgccgaat
     3661 ccggtaatgt cgtttttacc tcgcgcagta acgaccgcga ggcaatcatc aactgccccc
     3721 aaagttttcg cgcttcaacg taacgggcgt aaccggcatt attacgaaaa ccaagaaaaa
     3781 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttaatg cccaaatgcg
     3841 tgtaccaggg cagcatgaaa ataacagcga tcgaaaagag aaaattgagt agtaagcgcg
     3901 aggatatctt ggataatact gaaccgtgcc agacaaaaat acggcgcagc cagtgttgtt
     3961 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
     4021 tgatcacatt ctcaagatac tacttacaaa ttacctacaa gattacaaca ttaacaggtg
     4081 ctgcatgtgg cacctgttaa tgataaaggc atatagaatg ttgtaactaa tatagcggaa
     4141 taagaaaccc ggtcgaaacc gggttcagag tattagcaca acgggcgtac agcttcgcgc
     4201 atcccttttt cgagaatcgc atccaggtca ttagcaacct gctcgaccag accaggcact
     4261 tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac cagttcctgg
     4321 gtcccgataa cacggtcacg atactggctc cagagttgct gataacgttc cagccagtgt
     4381 gcatcgtcct gtaccggata tgtttctcca ttacgcacac cgcgatagaa cgcaatcaat
     4441 gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg ccctgccagc
     4501 agctgcggca ggatgcgtgt gcggaacttg gtcataccgt tgagcgcgat agacagcagt
     4561 tgatgcttga tgtacgggtt acggaaacgt ccggtgactg cactggcgaa agactccagc
     4621 tcatcgcgtg gcaaatccag taccggaata atttcttcgt agatggcttt ttcaacgaat
     4681 gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc ctggaacgcc
     4741 accggcacca gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc tttatacggt
     4801 ttaatatcgt caacgatcag cacgttaagc ggatatttgt ccagacgaag ttcagtcgct
     4861 aaggatttcg gcccctgaat cacaaacagg taaaagtgtt cagcggtgtc gaggaaacca
     4921 tcgtggtagc ccagttcttc ttccagttta gccacttcat cacgcggata accggtaacg
     4981 atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg aataaatgct
     5041 tcaggcaatg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc gtcgccatta
     5101 tagtcaatca actcacacgg gatgatgatc caacctttat ccagcgcacc gttgaaatgg
     5161 ctaaaacgtt cgaacagcag acgggtcagt tttgccggat aacttaccgc tggcgcgtca
     5221 tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga gaaaacaaag
     5281 cgcatttccg ggttgtgagc cagtttcagg aattcatcgt attcactata gacgctgatt
     5341 tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg cttcaccctt ctcattcagg
     5401 ccacggataa tggtggtata cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt
     5461 tcaatcggac gaacaacgac cacgccagaa ttcagatcgg tgtgctcatt caggagatcg
     5521 atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat gatacgttct
     5581 ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg ttcccttctg
     5641 attagtcata caacctgttt gaattggtac gacaggttag caaactttaa tacgtcgaac
     5701 ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag tgtggcgcgg
     5761 atcatggttt aatcgaggaa acaggccttt tcttggatca taaagtggta gaacacattg
     5821 catttaattc gctctgagta aataaagatg tcagacaact tcctcaccgt aacgcatagt
     5881 gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg cttcgaccat
     5941 caatttatta aagtcatcgg tcagcgttcg gtacgatgcg caaccactac cgacgccaat
     6001 acttaccgtg agataaagcg ttttttgttg ccaggtgaac ggttgcagtt caacgccttt
     6061 acggattttt tccgccatta gcaggccatc gacaggattc acagacggca ctgcaacagc
     6121 aaattcttcg ccgcccattc gcgccaccag ccccttatca ccgacaatct tctgaatatg
     6181 ctgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat cgttaatgct
     6241 tttgaaatag tcgatatcaa gcagcatgac cgtcagatgt tgtgtctgtt tcagcgatgg
     6301 acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgggtcagaa aatcaaaatc
     6361 ggctcgcagc gcaacttgct tcattagcga attgatcgct gccacgctaa aggaaaccat
     6421 aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt gcggaatgga
     6481 gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc accagtacga cttccacagc
     6541 gccggtgaca aaggtcaaca gacatgttac ttgcggcgta tagcgcactg cacaccagat
     6601 taatgcaggg agcggaaacg ccagactccc cgctccgcca atgactaccg aggcaataac
     6661 cgacacaata agcgcgatag caggcatcat ctgctctgct ttaaagcgcg gcagcactcc
     6721 aggaattgcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga attgctcact
     6781 aaaccagtcg gcaagcaaag gccagaaatc cagagtatca atactgaccg aaccaatcgc
     6841 cccgacaata gcgcataata atgcggcaag cagacagtaa ttaaataacc gtaaggcact
     6901 tactggctca tacttatttt ttcccagacg cttatcccgc gcgaccagta aggcgacggt
     6961 aacaataaac atcatattgg agaaattgat agccagtgaa accagccccc attcggtggt
     7021 tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca gtcgattaag
     7081 ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga aaagagaaag
     7141 ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta gcacaaaaat
     7201 agcgatgcta ttacgtagca gatgaccttc ctggaacaaa cggaaagttg aaatgggctg
     7261 tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa cgcaataagt
     7321 aaattcttag aaaggatagc atgttcgctg acaacggcgg caactaaaga tacctaatga
     7381 atgccatttt atcgatttcc gtaattatca tggctgattc atctgtataa ccagccagtg
     7441 atagattacc gtcacaatat aacgttgttg ctcctcactt aacgcttccc cctgaggaat
     7501 gttatgccac ttagccagac aaccgcgaca acaggtcgcg gtggcgtgtt gagcgataaa
     7561 aaccggatgc ccgcgcatgg gggtttgctt accgtcgtta gccggtaatg ccggagcaag
     7621 acgtttagcg acaaaatctg ccgcatgttg ttcaatgact ggcgcgcctt tctccagaca
     7681 atactgacgc tctttcacac ccaaacgaaa gcgagagcga aattttgaac gggataaacg
     7741 cgcgaagagt ggatcgagag actgcattaa taaactgaac gccctaattg tttcgtcaat
     7801 tgttcaagga cggcaatgcc cgcaagcgag ttaccggcat catccagttc cggactccag
     7861 acagcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac gccagatttt
     7921 gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc gctggtcgcc
     7981 atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca caggttcatc aatatgaata
     8041 gctttcccct gattcgccag aaagacaaac gtccgggcca gctctacaca gctcattttc
     8101 agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg gaaattgcca
     8161 aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc
     8221 gctaccaccg tatcgtagga aatatcagac acaccactta agccgcgcac gacttccagc
     8281 atacgttgcc gaggtgcgct taatcgccct tgcaacatat cgcagaccac cagcgcaccg
     8341 gcattaataa acggattacg cggaataccc tgctccattt ccagttgcac taaagaattg
     8401 aatggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc ggagtaatga
     8461 cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa acgttcttgc
     8521 gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc caatcgggaa
     8581 ccgtctaccg tagccagcgc cggaatataa tccgcgactt taccctgacc aatgagcggc
     8641 cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc cactttctac
     8701 tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat atagcgtcag
     8761 acccggtctt tccacaccgt ctggatatta cagaattcgt gtaagccgaa atgggaaagc
     8821 tcacgaccaa agccactctt cttcacgcca ccaaaggcga ctcgcgcatc gctggcacaa
     8881 taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct ggcctgtgtt
     8941 tcgtcggtgg tgaaaatggt cgctgaaagg ccgaactcac tatcattagc cagttccagc
     9001 gcatgttctg catctttcgc aatggtgatt gccgcaacgg ggccaaacat ttcttcccga
     9061 aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta gttacctgcc
     9121 ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gcgccagtgt tttttccacc
     9181 tgatgatgca gctcatcacg taaatcaaaa cgagccattg gtccgagagc gttgtcttca
     9241 tcacggggat cgcccatttt caaggctgcc gcagctgcca caaaacgttc ggtaaatgcc
     9301 gaagcaattc cctcttcgat gataaagcgt tttgccgctg cacaaacctg tccggtattc
     9361 tgataacgtc cggctaccgc cgctttcacc gccagttcca gatcggcatc gttaagcaca
     9421 ataaacggat ccgaaccacc cagttccagt acgcattttt tcagtgccgc tccagcctgt
     9481 gcgccaatag ccgctccagc acgaacactt ccggtcactg tgacagcagc aatgcgcgaa
     9541 tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata tacgccttgt
     9601 gggatacccg catctttaaa cacctgggca acgagctgtg cacagcccat cacattcggc
     9661 gcatgtttaa gtaagtagcc gttaccagca agaatgatgg gaacagcgcc acgcatcacc
     9721 tgccataacg gaaaattcca cggcataatc gccagaatcg tccccaacgg tcgatactca
     9781 ataaccgcct gctgattttc caccagcgta ggttctgcct tcagcattgc cggaccatgt
     9841 tctgcatacc agtcacacaa attcgccgat ttcgccactt cagcgcgcgc ctggttgatt
     9901 ggtttgccca tttcacgggt gatcatttgc gccatttctt cgctacgggc gcgcagagcc
     9961 ttaccgatat cacgcagttt ttcagcacga taatctatat ttgtctcgcg ccagtcgcga
    10021 aagcctgctg ccgccagctg aagtgcgttt tcgatatcgt caacaccagc ccacggcagc
    10081 acagaaagtt gttcaccggt ggcaggattt atcgaaattg catgagttgc cggagtaatg
    10141 gtcatcgggg tatctccttt atgagtcatg gtatgaagat acgcagattt actcttgctt
    10201 taaaatgaat aatattaagc cacttattca cgaatcgaga atgccatgga tctgacccaa
    10261 ctggagatgt tcaacgcggt tgccgaagct ggcagcatta cccaggctgc agcaaaagtg
    10321 catcgcgtgc cgtcgaatct cactacccgt ctacgtcagc tggaaacaga actgggggtt
    10381 gatctgttta ttcgcgagaa tcagcgttta cgtctctcgc ctgccgggca taacttttta
    10441 cgctatagcc aacaaattct cgcattagtg gatgaagcgc ggagcgttgt cgctggcgat
    10501 gagccgcaag gtttattttc tctgggatcg ctggaaagca ccgctgcagt gcgcattccg
    10561 gccacgctgg cggaatttaa ccgtcgttat cccaaaattc agttttcact ttccaccggc
    10621 ccttccggca ctatgctgga aggtgtgctg gagggaaaac tgaatgcggc gtttattgat
    10681 ggacccatta accatactgc catcgacggg ataccggtat accgcgagga actgatgatc
    10741 gtcacgccac aaggacatgc gccagtaacc cgtgccagtc aggtgaatgg cagtaacatt
    10801 tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg aaagctggtt tcatgctgac
    10861 ggtgccgctc cgggaactat tcacgagatg gagtcttatc acgggatgtt ggcctgtgtg
    10921 gtcgcaggag caggcattgc gcttattcca cgctctatgc tggaaagtat gccggggcat
    10981 caccaggttg aatcgtggcc gttagctgag caatggcgtt ggttaacaac ctggctggtc
    11041 tggcgtcgtg gtgcgaaaac gcgtccgctt gaggcattta ttcaactgct ggatgcgcct
    11101 gactcggcaa aacagggtta ccaatgagct atttttgata gttcatgcgg ctaatatgct
    11161 ctatatagtg atgttccaat gacttatgac tatatggggc aaatatggtt acgccagtaa
    11221 gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt gcacctcaag
    11281 taaaagaaga acaggccccc ttcctgatcg tcattaaaat gtgatgttaa caactctctg
    11341 atgcgtgaca gaacaaccgt cagagagatg gaaatgcgat ccagatcaca aatgcattgt
    11401 attcacatca ttaaccgttt taagatcatt tcatcacttt ttcgcaactc acccgataat
    11461 ctgttatgac aacaaacact gtttcccgca aagtagcgtg gctacgggtc gttacgctgg
    11521 cagtcgccgc ctttatcttc aataccaccg aatttgtccc tgttggcctg ctctctgaca
    11581 ttgcgcatag ttttcacatg caaaccgctc aggtcggcat catgttgaca atttatgcat
    11641 gggtagtggc gctaatgtca ttgcctttta tgttaatgac cagccaggtt gaacgccgca
    11701 aattactgat ctgcctgttt gtggtgttta ttgccagcca cgtactgtcg tttttatcgt
    11761 ggagttttac cgttctggtg atcagtcgca ttggtgtggc ttttgcacat gcgattttct
    11821 ggtcgattac ggcgtctctg gcgatccgta tggctccggc cgggaagcga gcacaggcat
    11881 tgagtttaat tgccaccggt acagcactgg caatggtatt aggtttacct ctcgggcgca
    11941 ttgtggggca gtattttggc tggcgaatga ccttcttcgc gattggtatc ggggcgcttc
    12001 ttaccctttt gtgcctgatt aagttacttc ccttgctgcc cagtgagcat tccggttcat
    12061 tgaaaagcct cccgctattg ttccgccgcc cggcattgat gagcatttat ttgttaactg
    12121 tggtggttgt caccgcccat tacacggcat acagctatat cgagcctttt gtacaaaaca
    12181 ttgcgggatt cagcgccaac tttgccacgg cattactgtt attactcggt ggtgcgggca
    12241 ttattggcag cgtgattttc ggtaaactgg gtaatcagta tgcgtctgcg ttggtgagta
    12301 cagcgattgc gctattgctg gtgtgcctgg cactgctgct acctgcggcg aacagtgaaa
    12361 tacacctcgg ggtgctgagt attttctggg ggatcgcgat gatgatcatc gggcttggta
    12421 tgcaggttaa agtgctggcg ctggcaccag atgccaccga cgtcgcgatg gcgctattct
    12481 ccgggatatt taatattgga atcggcgcgg gtgcgttggt aggtaatcag gtgagtctgc
    12541 actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc tgcttttgct gcgttaatat
    12601 ggtcaatcat catttttcgc cgctggccag tgacactcga agaacagacg caatagttga
    12661 aaggcccatt cgggcctttt ttaatgatac gttttaatga tttccaggat gccgttaata
    12721 ataaactgca cgcccataca taccagcagg aatcccatca gacgggagat cgcttcaata
    12781 ccacccttgc ccaccagccg cataattgcg ccagagctgc gtagacttcc ccacaaaata
    12841 accgccacca ggaaaaagat cagcggcggc gcaaccatca gtacccaatc agcgaaggtt
    12901 gaactctgac gcactgtgga ggccgagcta ataatcatcg caatggttcc cggaccggcg
    12961 gtacttggca ttgccagtgg tacaaaagca atattggcac tgggttcatc ttccagctct
    13021 tccgacttgc ttttcacttc cggtgaatca atcgctttct gttgcggaaa gagcatccga
    13081 aaaccgataa acgcgacgat caaaccgcct gcaattcgca gaccgggaat cgaaatgcca
    13141 aatgtatcca tcaccagttg cccggcgtaa tacgccacca tcatgatggc aaatacgtac
    13201 accgaggcca tcaacgactg acgattacgt tcggcactgt tcatgttgcc tgccaggcca
    13261 agaaataacg cgacagttgt taatgggtta gctaacggca gtaacaccac cagccccagg
    13321 cctattgctt taaacaaatc taacattggt ggttgttatc ctgtgtatct ggtttatcag
    13381 cgaaaagtat aaggggtaaa caaggataaa gtgtcactct ttagctaacc ttgcatcgca
    13441 ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg gtcaattcat tcatttgact
    13501 tatacttgcc tgggcaatat tatcccctgc aactaattac ttgccagggc aactaatgtg
    13561 aaaagtacca gcgatctgtt caatgaaatt attccattgg gtcgtttaat ccatatggtt
    13621 aatcagaaga aagatcgcct gcttaacgag tatctgtctc cgctggatat taccgcggca
    13681 cagtttaagg tgctctgctc tatccgctgc gcggcgtgta ttactccggt tgaactgaaa
    13741 aaagtgttgt cggtcgacct gggggcactg acccgtatgc tggatcgcct ggtctgtaaa
    13801 ggctgggtgg aaaggttgcc gaacccgaat gataagcgcg gcgtactggt aaaacttacc
    13861 accagcggcg cggcaatatg tgaacaatgc catcaattag ttggccagga cctgcatcaa
    13921 gaattaacaa aaaacctgac ggcggacgaa gtggcaacac ttgagcattt gcttaagaaa
    13981 gtcctgccgt aaacgaaaaa agaggtatga cgatgtccag acgcaatact gacgctatta
    14041 ccattcatag cattttggac tggatcgagg acaacctgga atcgccactg tcactggaga
    14101 aagtgtcaga gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa
    14161 ccggtcattc attaggccaa tacatccgta gccgtaagat gacggaaatc gcgcaaaagc
    14221 tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc gagtcccaac
    14281 aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga
    14341 ccaatatgca gggtgaatcg cgctttttac atccattaaa tcattacaac aactagttga
    14401 taacgtgaca acgtcactga ggcaatcatg aaaccacttt tatccgcaat agcaactgcg
    14461 cttattctct tttctgcgca gggcgttgcg gaacaaacca cgcagccggt tgttacttcc
    14521 tgtggcaatg tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac
    14581 atgggtactg gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct
    14641 atgtagtttg ttctggcccc gacatctcgg ggcttattaa cttcccactt ttaccgcttt
    14701 acgccaccgc aagccaaata cattgatata cagcccggtc ataatgagca ccgcaccgaa
    14761 aaattgcaga cccgttaagc gttcatccag caatagtgcc gcacttgcca gtccaactac
    14821 aggcaccagt aacgataacg gtgcaacccg ccaggtttca tagcgtccaa gcagtgttcc
    14881 ccagatccca taaccaacaa ttgtcgctac aaatgccaga tacatcagag acaagatggt
    14941 ggtcatatcg atagtaacca gactgtgaat catggttgcg gaaccatcga gaatcagcga
    15001 ggcagcaaag aagggaatga ttgggattaa tgcgctccag attaccagcg acatcaccgc
    15061 cggacgcgtt gcgtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa
    15121 tgctgccgcc agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc
    15181 ttcgattaac accagtacgc caaaaatcgc taaggcgatc cccgtcaatt gtttgccatg
    15241 cagtcgctca ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa
    15301 caccagcgaa gccagtccag caggcatacc aaagttaatg gcgcagaaca gaaaagcaaa
    15361 ctgcgcaaaa ctgatggtta atccataccc cagcagcaaa ttcagtggta ctttcggtcg
    15421 tgcgacaaaa aagatggccg ggaaagcaac cagcataaag cgcaaaccgg ccagcatcag
    15481 cggtggcatg ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacaaccac
    15541 taccagtagc gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt
    15601 ttgtaagcaa tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcaccg
    15661 tttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc catgctattc
    15721 ctttacatct ctaaaacaaa acataacgaa acgcactgcc ggacagacaa atgaacttat
    15781 ccctacgacg ctctaccagc gcccttcttg cctcgtcgtt gttattaacc atcggacgcg
    15841 gcgctacgct gccgtttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc
    15901 taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg
    15961 gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg
    16021 ctagcggttt tattgccatt cctttagtga ataacgtgac gctggtggtg ctcttttttg
    16081 cccttattaa ctgcgcctac tccgtttttg ctaccgtgct gaaagcctgg tttgccgaca
    16141 atctttcgtc caccagcaaa acgaagattt tctcaatcaa ctacaccatg ctcaacattg
    16201 gctggaccat cggtccgcca ctcggcacgc tgttggtgat gcagagcatc aatctgccct
    16261 tctggctggc agctatctgt tccgcgtttc ccatgctttt cattcaaatt tgggtaaagc
    16321 gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac
    16381 aagataaagc actgttgtgg tttacctgct ctggctttct ggcttctttt gttagcggcg
    16441 catttgcttc atgcatttca caatatgtga tggtgattgc tgatggtgat tttgccgaaa
    16501 aggtggtcgc ggttgttctt ccggtgaatg ctgccatggt agttacgttg caatattccg
    16561 taggccgccg ccttaacccg gctaacatcc gcgcgctgat gacagcaggc accttctgtt
    16621 tcgtcatcgg gctggtcggt tttatttttt ccggcaacag cctgctaatg tggggtatgt
    16681 cagctgcggt atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg
    16741 accatattgc gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc
    16801 ttggtgctgc gattaaccca ttagtgagtg gcatagtact taccagtctg ccgcctttct
    16861 cgctgtttat catcttagcg ttggtgatcg ttgttgcgtg ggtgctgatg ttaaaaggaa
    16921 ttcgtgcaag accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataagaa
    16981 tttaattcag ccttcgttta ggttacctct gctaatatct ttctcattga gatgaaaatt
    17041 aaggtaagcg aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga
    17101 atgtttttat ggccgtactg ggaataattt tattttctgg gtttctggcc gcgtatttca
    17161 gccacaaatg ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac
    17221 agttgtgtac aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg
    17281 gtgaatgctt gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca
    17341 ctgataacgg atcgttgaca cagtagcatc agttttctca atgaatgtta aacggagctt
    17401 aaactcggtt aatcacattt tgttcgtcaa taaacatgca gcgattcctt ccggtttgct
    17461 taccctcata cattgccctg tccgctcttc caatgaccac atccagaggc tcttcaggaa
    17521 atgcacgact cacaccagca gtcacggtaa tgttgatatg cccttcagaa tgtgtgatgg
    17581 catggttatc gactaactgg caaattctga cacctgcacg acatgcttct tcatcattag
    17641 tcgctttgac aataatgata aattcttcgc ccccgtagcg ataaaccgtt tcgtaatcgc
    17701 gcgtccaact ggctaagtaa gttgcgaggg tgcgtaatac tacatcgccg attaaatgcc
    17761 cgtaggtatc attaaccaat ttaaatcggt ctatatccaa caacattaaa taaagattca
    17821 gaggttcagc attgcgtaac tgatgatcga aggattcatc aagaacccga cgacccggca
    17881 atcccgtcag aacatccata ttgctacgga ccgtaagcaa ataaattttg taatcggtta
    17941 atgccgcagt aaaagaaagc aacccttcct gaaaagcgtc gaaatgcgcg tcctgccagt
    18001 gattttcaac aatagccagc attaattccc gaccacagtt atgcatatgt tgatgggcag
    18061 aatccattag ccgaacgtaa ggtaattcat cgttatcgag tggccccaga tgatcaatcc
    18121 accgaccaaa ctggcacagt ccataagaat ggttatccgt tatttctggc ttactggcat
    18181 ctctcgcgac cacgctgtga aacatactta ccagccactg gtagtgggca tcgatagcct
    18241 tattgagatt taacaagatg gcatcaattt ccgttgtctt cttgatcatt gccactcctt
    18301 tttcacagtt ccttgtgcgc gctattctaa cgagagaaaa gcaaaattac gtcaatattt
    18361 tcatagaaat ccgaagttat gagcatctct gagataacat tgtgatttaa aacaaaatca
    18421 gcagataaaa aagtgtttaa ttctataaat taactctgca ttatcgcaat taaaaggatg
    18481 acaaatagca taacccaata ccctaatggc ccagtagttc aggccatcag gctaatttat
    18541 ttttatttct gcaaatgagt gacccgaacg acggccggcg cgcttttctt atccagactg
    18601 ccgctaatgt tgatcatctg gtccggctga atttgaaaat tattataacg gaagagtata
    18661 caaaaaaagt aattttgctg gcgactgcga aacggcattc gcattagcgg tgaagtttgc
    18721 cctcgcccag cggctgcaat tactgctata acgaggctgg tctggcgata aatccaggcc
    18781 agccattggt ggtgtttata cgttcaagcc acgatgttgc aacatcggca taatctgagg
    18841 tgccttaccg cgccattgtc ggtacaggcg ttccagatct tcgctgttac ctctggaaag
    18901 aatcgcctcg cgaaaacgcc gcccattttc acgcgttaat ccgccctgct caacaaacca
    18961 ctggtaacca tcatcggcca acatttgcgt ccacagataa gcgtaataac ctgcggcata
    19021 tccgccacca aaaatatggg cgaaataact gctgcgatag cgtggcggta tagcaggaag
    19081 atccatattt tccgccacca gcgcccgcaa ttcgaaatca tcgacatcct gcattgcttc
    19141 gttttcttcc aggcaatgcc agcgcatatc gagaagtgcg gcgctaagca gttcgctcat
    19201 ctcataccct ttattgaaca ggctggcatt acgcattttc tgttgaagtt cgtcaggcat
    19261 tgcggtcccg ctctgataat gccgggcgta gcgagcgaat acctgcggat gtgttgccca
    19321 gtgttcgttg atttgcgacg gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag
    19381 cgtggcataa cgctggcggg caaaaaggcc gtgcagcgta tgaccaaatt catggaataa
    19441 ggttattaca tcatcccaga gtaacaacgc aggctcaccg gcagcgggtt tctgataatt
    19501 gcagacgtta taaattaccg ggtgcgtttc attcagcgtt gattgctcaa caaaattgcc
    19561 catccatgcg ccgccgcttt ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag
    19621 ccccacgcca ttatgatcaa aaatttccca cacacgaacg tcaggatggt agacaggaat
    19681 atcaaaacgt tcgacaaact taataccgaa gagctgattc gcagtccaga atacgccttc
    19741 attcaacacc gtgtttaatt caaaatatgg cttgagctgc gcttcatcga gatcgaattt
    19801 ctcacgtctt acctgctcgg cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc
    19861 ctgctgctta tcgataaccg cctgtatgga ggctaattca tcgctcgcac gttgacgcgc
    19921 cgctggaaca atttcccgca taaagttgag cgctgcttct ggcgtttttg ccatctgatc
    19981 ggcgattttc catgcggcat aatgaggaaa accaagcagt ttcgcctgct gcgcgcggat
    20041 ctccaccaga cgttgaatga tagcgcgggt atcattggca tcattttttt ccgctcgcct
    20101 ccagcccgca gtaaacagtt tttcacgcgt cgcgcgatcg cgcagttcag caagcgctgg
    20161 ttgctgggtg gtattcagca gcggaatcag ccagttgtta tccagacctt tctcgcgagc
    20221 cgcctctgcc gccagcgcaa tctcttgttc actcattcct gccagctgcg cgatatcgtt
    20281 cacaaccaga ccgccggatt tatttgctgc cagtaaccgc tggttaaact ggctggtcag
    20341 ggtcgcagct tctgtattca gtacttttaa ttttgcttta tcagcttgct caagtttggc
    20401 tccggcaagg acaaaacgtt gatgaatcac ctccaccagg cggatggatt cactatcaag
    20461 ccccagggat tcacggcgtt gccagacagc atctacccgc gcgaataatt ccccgttcag
    20521 atagatatca ttcgcaagtt ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa
    20581 ttcatcattg gtatgcgccg cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa
    20641 ttctccgctt tgttccagtg ccagaatagt attgttgaaa tcaggtgttt gcgggttaag
    20701 cgcgatggca gcaatttctg cccgcttttg ctgcattccc tcatcgaatg ccgggcgata
    20761 gtgatgattg gcaatttgat caaaatgggg agccagatac ggcagtgtgc tttgcacaag
    20821 gaaaggattc attgttgtca ttttcttctc ctgaacgcga ggtgttccat agcgtaggct
    20881 tactgataac ggagtgcaat cttgcaaacc agtattaccc gctcttaagc atcacgtgct
    20941 atgttattga cacacaaaat cgttgaggaa cagtgagatg atcgttttag taactggagc
    21001 aacggcaggt tttggtgaat gcattactcg tcgttttatt caacaagggc ataaagttat
    21061 cgccactggt cgtcgccagg agcggttgca ggagttaaaa gacgaactgg gagataatct
    21121 gtatatcgcc caactggacg ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct
    21181 ccctgccgag tggagcaata ttgatatcct ggtaaacaat gccggcctgg cgttgggcat
    21241 ggagcctgct cataaagcca gcattgaaga ctgggaaacg atgattgata ccaacaacaa
    21301 aggcctggta tatatgacgc gcgccgtctt accgggtatg gttgaacgta atcatggtca
    21361 tattattaac attggctcaa cggcaggtag ctggccgtat gccggtggta acgtttacgg
    21421 tgcgacgaaa gcgtttgttc gtcaatttag cctgaatctg cgtacggacc tgcatggtac
    21481 ggcggtgcgc gtcaccgaca tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt
    21541 ccgctttaaa ggcgatgacg gtaaagcgga aaaaacctat caaaataccg ttgcattgac
    21601 gccagaagat gtcagcgaag ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat
    21661 caatacactg gaaatgatgc cggttaccca aagctatgcc ggactgaatg tccaccgtca
    21721 gtaattttta tacccggcgt aactgccggg ttattgcttg tcacaaaaaa gtggtagact
    21781 catgcagtta actcactcac aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca
    21841 cacagcctgt caatcagcag atttatcgta ttcttcgccg cgacattgtc cattgcctga
    21901 ttgcaccagg cacaccgttg tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc
    21961 agccggttcg tgaagccttt attaaactgg cggaaaacgg tctgattcaa attcgtccgc
    22021 agcgtggcag ctacgtcaac aaaatttcca tggcccaggt gcgcaacggc agttttatcc
    22081 gtcaggccat tgagtgcgcg gtggcgcgtc gggcggcgag catgattacc gaaagccagt
    22141 gctaccaact ggaacaaaat cttcaccagc aacgcattgc cattgagcgc aagcaactgg
    22201 atgatttttt tgaacttgat gacaacttcc atcaactcct gacgcagatt gccgactgtc
    22261 aactggcgtg ggataccatt gagaacctga aagcgaccgt tgatcgcgtg cgctatatga
    22321 gcttcgacca cgtttctcca ccagaaatgc tgttacgcca gcatcttgat attttctctg
    22381 ccctgcaaaa acgtgatggc gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa
    22441 tcagcgaatc cgtgcgccag atccgccagg aaaacagcga ctggtttagc gaagagtaat
    22501 tcatttcctc tcatcccatc cggggtgaga gtctttcccc ccgacttatg gctcatgcat
    22561 gcatcaaaaa agatgtgagc ttgatcaaaa acaaaaaata tttcactcga caggagtatt
    22621 tatattgcgc ccgttacgtg ggcttcgact gtaaatcaga aaggagaaaa cacctatgac
    22681 gacctacgat cgtaaccgta acgcaatcac cactggcagc cgtgttatgg ttagcggcac
    22741 cggtcacact ggcaagatcc tgtcgattga tactgaaggt ctgaccgctg agcaaatccg
    22801 ccgcggaaaa accgtagttg ttgaaggttg tgaagagaaa ctggcaccac tggacctgat
    22861 tcgtctcggc atgaactaag cgtgtgaatg ccgccgatgg cggcattgct ttttttactt
    22921 cacggaatat ttcgccactg tcgctttcgc gccatgcgct aataaagaca agtacgcttc
    22981 cgtcacttta gtcgtaaaca agctattatc tggcaaatca tcaccaaata tcgccttaat
    23041 cgccaacaat gactgcacgc gcgctgtccc ttcagcacta ctttgtactg ccttctgaat
    23101 aacaggtaac agtgggtcac tgatttctat cggatttccc tgttcatcaa caccaccgac
    23161 ataacgcatc caacccgcga cgcccagcgc cagcagatcg aacttgctgt catgcgccag
    23221 atgccagcga acagaatcca acatccgctg tggcaatttc tggctgccat ccatcgcaat
    23281 ctgccaggtt cgatgacgta acgccgggtt gctatagcgt tcaattaatc ggttagcgta
    23341 atcttgcaaa tcaacgccct gcactttcag cgtcggcgct tgttcctgca acattaaggt
    23401 atacgccgca tgacgataat gttcatcctc catgcagtca ttaatgtgct gatatccggc
    23461 aagatagccc agatacgcca ggaatgaatg actgccattg agcatgcgca acttcatctc
    23521 ttcataaggc agcacatcac taaccagttc gactcccgct ttttcccatt ccggacgtcc
    23581 ggcaacaaag ttatcttcta ttacccactg gcggaaaggt tcacaggcaa cgcctgccgg
    23641 atcgcgcaca ccggtaagtt gttcgatttt cgccagcgta tcctctgtca ctgcgggcac
    23701 aatacggtcc accattgttg atgggaaagt cacgttatct tcgatccatt gtgccagttt
    23761 tacatcaatg gcttgcgcat aggaagtgac aacgtcacgc ataacatgac cgttttctgg
    23821 catgttgtca catgacatga cggtaaatgc gggaagtcct gccgctttac ggcgagccag
    23881 cgcctcaaca atcacccctg ttgcagtttt cggctggtgg ggattttgca cgtcggcaac
    23941 gaccatcggg tgatcgagca ttaactgtcc ggtcgccgga gagtggaaat accctttttc
    24001 ggtgattgtc agagagacaa tcgcgatttg cggttcacac atcgcagcca acacggtttc
    24061 taagccgtct atttgtacgt gtaaggcttt tttaacgacg ccaacgacgc gagccgtcca
    24121 cgcatcagcc gacatttccg caacggtata aagattatct tgctgttgta aatcggcaat
    24181 ttgctgttcg ccgccgatta agttgacctc ataatatccc cagtcactga aatgttccgt
    24241 agcaagaata tcggcataca cgccctgatg cgcacggtga aatgcaccaa agcctaaatg
    24301 aacaattctt ggggccaggt tattacgatc ataaacaggg agcgtcgctt ttgctgataa
    24361 caaatgattt cccataacaa ttccttaaaa ttaaatatag caagctacat gtttttttat
    24421 atgaataaaa atcccctctc cggtaagaga agggattgag gatttacaga cttctggacg
    24481 gttgcgcagc tcttacaaca tgtggttgat cttctgcggc gtcttgcagc gcacttaaat
    24541 cacggtcttt cacttctggc attttcagcg cagagattaa accaatcaca gaatatgcca
    24601 tgatcataat ggcgatcgga taccaggatt ccgtcatggt gcagaaaatc cccgccagga
    24661 taggaccaaa accggaagcg ataagaccac caatttcttt agaaatagcc attcgggtaa
    24721 agcggttttt acagccgaac atttctgcca tggtaatgtt ttccagagca aataatccaa
    24781 gcaccgcaca gttatgaatc acaatcagtg caaccataat ggtgctcggg gcatagcttt
    24841 tatctacgat gatagaaagc attggccatg ccagcacaat cgcagaggta ttcataataa
    24901 tatacgggat ccggcgacca attttatcgg ataaccaacc aaggaacgga atggtcataa
    24961 agccgagaat cgaactgatc atcaatgcat ctgttggaat tgctttgtta aacaataacg
    25021 tctgcactaa atagcctgca aggaaagtct gaattaaacc tgagttacca gcctgaccaa
    25081 aacgcagccc tgttgccagc cagaaggatt tgctctggaa catgctacca gcaggtgcag
    25141 gttttgctgt cggttggttg ctgtcgttaa ccttctcaaa gaccgggctt tctttcagat
    25201 tcatacgcaa ccagatagca aagaccatca cgacaacgct cgccaggaaa ggtatacgcc
    25261 atccccacgc cagcagttcc tctttactga gaatgaagaa cataaaggcc cagattgccg
    25321 ttgcgctcaa ggttccgcag ttagttccca tagccacaaa tgaggagata attccgcgct
    25381 taccttttgg cgcatattcc gccagcatcg taccggcacc ggaaatttcc gcacctgcac
    25441 ccaacccctg aataatacgc agcgtcacca gcaagatggg ggcaaaaaca ccaatttgtg
    25501 cataagtcgg taacacacca attaaggtgg tacagatccc catcatggtg atggtaataa
    25561 agagcacttt tttacgccct attctgtcgc ccattttgcc gaaaataaat gctccgacaa
    25621 tacgtgccac ataacctgca ccataggttc ccattgccag aattaacgcc attgccgttg
    25681 atgattcagg aaaaaatatt tcatgaaaca ctaacgctgc gccgagcgaa tataactgga
    25741 aatccataaa ttcaagcgca gtgcccagcc atcccgatac tgctgctttc accaaatcct
    25801 tagtgcttct ttcgtgtttt tctattgtca taatggttat ctctaaaaaa gaggtaagat
    25861 gcgtactact tattcgccgt tattggtatt attcagaaaa agtgagtaag actttgcagc
    25921 aatgtttttg atcctgttca aataaactaa tggcatccgc aacatgctgg aaatcaaacg
    25981 tatgggtaat tagtttttct ggtttaatta accctttact taaccagtcg ataacgaccg
    26041 ggaatttatt tgcatttaag cgtgaagaga aaatagagag ttcttttccg gtaattcctt
    26101 gctgaatcac ttcagacggt tcactggaga agcccatcaa tacaatacgt gccgctggag
    26161 aagccagcgt tacggcctct ttcaagattg aaggatgaca agcggcatcg ataactaatg
    26221 tcggttggat gcctttttca tcgaaaatct cgccaagtgg tgtctggctg ttattaatcg
    26281 cccagtctgc cccgctctct ttcgcttttt ccagtcgttc atcaatgcga tcggcaacaa
    26341 tcacattttt aacgttatag acgcctttta atacctgaac gatcgtcagg ccgattggac
    26401 cggcaccgta aaccagaacg gtatcatttt cagtcggttg accatgaccg gtaacgttag
    26461 ccgcaatggt aaaaggttcg atcatcaccg catattgatc ggccactgct tcaggaattt
    26521 tccacgcatt ttttgccgga accacggcat attcactgaa accaccgtca gcgtgcacac
    26581 ctaatacagc aagtgtcgta caaacgttcg gcttacctat agagcacgga tagcaatgcc
    26641 cacagctgac caccggatcg acagcaacgc gttcaccgac tctggcgctt tccacgcctt
    26701 cacccactgc atcaatgacg ccaaagaatt catgaccaat gacgcgcgga tatttcgcaa
    26761 aaggattatg cccacggtaa atatggctat ctgaaccaca aattccggca agtttcactt
    26821 ttactcgtac ttcacccgct gacggggtgg gtatttcacg ttcgataatc gacagttgat
    26881 tcggtttttc aattaaaatg cttttcatta ccttactcct taccagttcc acagcgtgcc
    26941 atcttccaga cgtgcgactg gcagataagc aggttcatag ggatatttcg ccgccagctt
    27001 ttcatcgaat tcgatgccaa gacccggttt ttctcccgga tgcatatagc cgttatcgaa
    27061 agtccagttg tgcgggaaga cttcgagcat ttgttcggaa taacccatgt attcctggac
    27121 accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa
    27181 atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat cggcaatccg
    27241 acgcataccg gtaattccgc ctgcatgagt cagcgtggtg cggatataat cgatgagttg
    27301 ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca ccgcgatggg
    27361 tgtgacggta tgttggcgaa tgagacggaa gcattcctgg ttttccgcag gcgtcgggtc
    27421 ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg cttcaatagg
    27481 cgttaagcga tggtgcatgt catgcagcaa atgttcatta aatccaaact tgttacgtac
    27541 cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc
    27601 cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg acatgccgta
    27661 ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc ccagctcctg
    27721 atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat ggcaataaac
    27781 catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt tggcagcttt
    27841 ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg taaccggacc
    27901 gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac ggtgtgcatc
    27961 gcgaccaata agctgcggac aaaggtgatc ctgcaaataa gaggccacgg aaagctcacg
    28021 tccatttagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga tttttaatgt
    28081 gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct tcatgttcga
    28141 ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgaactact accatacaag
    28201 tatgaagatc aaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg cgccgtgaat
    28261 ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg tatcttattg
    28321 ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc aacatgccgc
    28381 aaagcgcaat taacgccccc gtccagtcat aaagagtcag tttcacgcca tccacaacgc
    28441 gcagccacat caacgccgtg caaacataaa cgccaccata agccgcgtaa acacgcccac
    28501 tcgccgcagg atgcaacgtt aacaaccaga caaacagtgc cagtgaaatc cccgccggaa
    28561 gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag catccaataa
    28621 tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt gtcacttatt
    28681 gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacaccc tccttgaacg
    28741 ttttgcgttc gcagtggatg gtgtagaatc agctttaatc attcatacag ggaatgaatt
    28801 atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc ttgcgcactg
    28861 gtattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc tggcgacagt
    28921 gcacaaagcc gccagcgcgc cgctatggaa aaagagcaat ggaatgacac gcgtaatctg
    28981 cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc tgcttttgat
    29041 aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc aaatactttg
    29101 cgctgcctgg accgtcgaac tggccgcgtt attatcccct aacctgttat tgatttaagg
    29161 aatgtaagga cacgttatgc caagcgccca cagtgttaag ctacgcccgc tggagcgtga
    29221 agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga
    29281 ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcata ttcacgatca
    29341 gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt
    29401 ggaaattaac catgttcatc gccgcgcaga atttcagata attatctccc cggagtatca
    29461 ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct tcaccgttct
    29521 caatctctat aagctgtatc tgatcgttga taaagagaat gaaaaagcga ttcacattta
    29581 ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagttcttta ttaatggtca
    29641 atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggctg agcacaaaac
    29701 accgggtcag actctcctga agccgaccgc acaatagtat taataataat cgatcgtatt
    29761 tttgatggta taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgattagctg
    29821 acagtccacc ggattggcgt gattgtcata ttcacagctc tgttttacat taccaacccg
    29881 ttgattattc agtaaagtaa cagcagtgta atctaatttt ttgatcgggt ccgttgatgg
    29941 cgtggcgctg acagataatg ttttatcgtt acttttcgtg gttttaccca gcggataccc
    30001 ctgatcatca tagcgatatt ccatctgcat ctgtttgctg ctggctttaa tcacgaaacc
    30061 attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct ggcattttcc
    30121 ctgcaaacga actcttttct ccagcgtctc ggcatcacgg taataattgg catccagtac
    30181 cagggcaacc aaggtattat tttccagatc cagtaattcg agagaatcaa aacagccttc
    30241 ttccgacaaa gtccccgaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg
    30301 agtgaaatct tttacgggac cacgcagcgg atcaaaatcg aattcattag agaaactggc
    30361 catctccggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga gtaatatcgc
    30421 gagtaagcat ggcagtaatt tgtatttcac aacagtcacc tgagagtaga gatgattctc
    30481 aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt
    30541 cgcgtaagga gcttatgatg aaacactcaa cctactgtgc cgcgcttctg ctcgccctcg
    30601 cctcacccac ggtactcgcc gcgccgggat cctgtgagcg catacagagc gatatatcac
    30661 agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg
    30721 accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat acgcataaaa
    30781 ttctctacac ccgtaccacc agcggtaacg tctctgctcc cgcgcagtct acccaggatg
    30841 gcgcgcctgc cgaaccgcag taacacatta tcgactgaac gccggatgcg acacatccgg
    30901 cgatttgaac attcaacata accccacctt attactcata cccttctatt gatatggatt
    30961 aataattatt aaccccaaat gggtagattc cctctattgt tagcgcgcta aatattcaat
    31021 atataaacat ttatataacg ataaagaaca gggagtgagt tatgtccaaa aatgaacaaa
    31081 tggtgggcat cagccgcaga acactcgtta aatctactgc gataggttct ctggcgctgg
    31141 ctgcaggcgg tttttctttg ccgtttaccc tgcgcagtgc agcagcagcg gtacaacagg
    31201 ccagcgaaaa agtggtctgg ggtgcctgtt ccgtcaactg tggtagccgc tgtgcacttc
    31261 gtctgcatgt taaagataat gaagtgacct gggtggaaac tgacaacacc ggcagcgaca
    31321 agtatggcaa ccatcaggta cgcgcctgtt tgcgcggtcg ctccatccgt cggcgtatta
    31381 atcatcccga tcgcctgaat tacccaatga aacgcgtggg caaacgcggc gaaggcaaat
    31441 tcgaacggat tagctgggat gaagccctgg atactatcgc cagtagcctg aaaaaaaccg
    31501 tcgaacaata tggcaatgag gctgtatata ttcagtactc ttcggggatt gttggcggca
    31561 atatgacccg ctcttctcca tcagcctcgg cggtcaaacg cctgatgaac tgctacggcg
    31621 gttcactaaa ccagtatggc tcctacagca ctgcgcaaat ttcctgtgcc atgccctaca
    31681 cctacggcag taatgatggc aacagcacca cggatattga aaacagcaag ctggtcgtga
    31741 tgtttggtaa caacccggct gaaacccgca tgagcggcgg tggcatcact tatcttcttg
    31801 aaaaagcgcg cgagaaatcg aacgcaaaaa tgattgttat cgatccgcga tataccgata
    31861 cggctgctgg tcgtgaagat gaatggcttc ctattcgccc gggcaccgat gccgcgctgg
    31921 ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt tgatcaacct tttctcgata
    31981 aatactgcgt cggctatgac gaaaaaacct tacccgcaga tgcacccaaa aatggtcact
    32041 ataaagccta tattcttggt gaaggtgatg ataaaacagc gaaaacgccg cagtgggctt
    32101 cgcaaattac aggtatcccg gtagaccgta tcatcaaact ggcgcgtgaa attggcacag
    32161 caaaacccgc ttatatctgc cagggctggg ggccgcaacg gcaggcaaac ggcgagctga
    32221 cggcacgcgc tattgctatg ttgcctattt tgacgggcaa tgtcggcatc agcggcggaa
    32281 atagtggtgc gcgtgaatcg acctacacca ttaccatcga acgcctgccg gtgctggata
    32341 atccggtcaa aacgtcaatc tcctgcttca gttggacaga tgctatcgat catggcccgc
    32401 aaatgacggc aatccgcgac ggcgtccggg gcaaagataa actagatgtg cccattaagt
    32461 tcatctggaa ctacgcggga aataccctgg ttaatcagca ttctgacatc aacaaaacgc
    32521 atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt ggtcatcgaa aactttatga
    32581 cctcatcggc aaaatatgcc gacattctgc tgccagacct aatgaccgtt gagcaggaag
    32641 atattattcc taacgactac gccggtaaca tgggatatct cattttcctc cagcctgtca
    32701 ccagcgaaaa attcgaacgc aaaccaattt actggatcct gagtgaagtc gcgaaacgtc
    32761 ttggacctga cgtctatcaa aagttcactg aaggtcgcac gcaagaacaa tggttacaac
    32821 atctgtacgc caaaatgctt gccaaagatc cggcgttacc gtcttacgac gaattaaaaa
    32881 aaatgggtat ctataagcgt aaagatccca atggtcattt tgtcgcctac aaagcatttc
    32941 gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg taagattgaa atttattcca
    33001 gcaagctggc ggaaattgcc cgtacctggg aactggaaaa agatgaagtg ataagcccac
    33061 tgccggttta tgcctcaacc tttgaagggt gggactcccc tgaacgtagt accttcccac
    33121 tgcaactgtt tggtttccat tacaaatccc gtacgcactc gacctacggc aacattgatg
    33181 tcctgaaggc agcctgccgt caggaagtgt ggatcaaccc tatagatgca cagaaacgtg
    33241 ggattgccaa cggcgatatg gtgcgggtgt ttaaccatcg tggcgaagtt cggctaccag
    33301 ccaaagtgac accacgcatt ctccctggag ttagtgctat gggccaggga gcctggcacg
    33361 aggccaatat gtctggcgat aaaatcgacc acggcggctg tgtgaatacg ctaaccactc
    33421 tgcgcccttc accactggcg aagggaaacc cgcagcacac taatctggtc gagatcgaaa
    33481 aaatataacc cactacggcc ataaattttg acatgacatt ttgtttgaaa agcaataagt
    33541 gagtaatgat gaaaatccat aacacagagg cgctcatgtc ggctgaaatc agtcgtagaa
    33601 gtctgatgaa aacctccgca cttggcagtc tcgcgctggc aagcagtgct ttcactttgc
    33661 cgttttccca aatggtccga gcggcacagg caccggtaga agagaaagcg gtctggagtt
    33721 cctgcaccgt taactgcggg agccgctgtc tgttacgttt gcatgtgaaa gatgacaccg
    33781 tgtactgggt ggagtctgat acgacaggtg acgacgttta cggtaatcat caggttcggg
    33841 catgtttacg cgggcgctct atccgccgac ggatgaatca tcctgacagg ttgaaatatc
    33901 ccatgaagcg cgtcggcaag cgcggtgaag gtaaatttga acggataagt tgggacgaag
    33961 ccctggatac catcagtgat aatcttcggc ggatcctaaa agattacggc aatgaggctg
    34021 tacatgttct ctatggaaca ggcgtagatg gcggaaacat caccaactca aacgtcccgt
    34081 accgtctgat gaactcttgc ggtggttttc tcagtcgcta tggcagctac agtaccgcac
    34141 agatcagtgc cgcaatgagt tatatgttcg gtgccaatga tggcaacagc ccggatgata
    34201 tcgccaatac gaaactggtc gttatgttcg gaaataaccc gtcagaaacg cggatgagcg
    34261 gctttccgtc tttacatgga cagatgccat cgaccatggt acggaaatga ccgcgacccg
    34321 tgatggtgtt cgaggaaaag aaaaactgga tgtccccatc aagtttttat ggtgctacgc
    34381 cagtaacaca ttgatcaatc agcatggcga catcaatcac acccatgagg tgcttcagga
    34441 tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgactgcct cggctaagta
    34501 ttgcgatatc ctgttacccg acctgatgcc aacagagcaa gaagacctta tctctcatga
    34561 atctgccggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag caaaatttga
    34621 acgcaaaccc atctactgga tgctgagtga agtcgccaaa cgcttaggac cagacgttta
    34681 tcaaaccttt actgaaggtc gcagtcagca tgaatggatc aaatatctcc atgcgaaaac
    34741 gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg ggatctttaa
    34801 gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgtag atccacaggc
    34861 aaacccgttg aaaacacctt cgggaaaaat cgaaatttat tctgaacgac tggcgacaat
    34921 tgcggatacc tgggaattga aaaaagatga aattattcat ccactgccag cgtatacccc
    34981 aggtttcgac ggttggaacg atcccctacg ggggacctat ccactgcagt taacggtatt
    35041 ctgacctctc accgcccttc accgctggca aagggaaacc cgtcgcacag caatcttgtc
    35101 cagatcgaaa aggtttaagg agtagcccat gaccacacaa tatggatttt ttatcgattc
    35161 cagccgctgt actggctgta aaacctgcga actggcgtgc aaagatttta aagatcttgg
    35221 cccggaagtc agtttccgcc gtatttatga atatgctggc ggcgactggc aggaagataa
    35281 tggcgtctgg catcagaatg tctttgccta ctatctctca atttcgtgca accactgcga
    35341 tgatccggcg tgtaccaaag tctgccccag cggcgcaatg cacaaacgag aagatggctt
    35401 tgtggtggtc gatgaagatg tttgtatcgg ctgccgctac tgccacatgg cttgtccgta
    35461 cggcgcgcca cagtacaatg ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc
    35521 gcgcgtcgcc gaggggaaac aacccatatg tgtcgaatcc tgcccactgc gtgcacttga
    35581 gttcggtcca attgaagaat tgcgccagaa gcacggcact ctggctgccg tcgcgccact
    35641 gccgcgcgcg catttcacaa aacccaatat cgttatcaaa cctaacgcca acagccgccc
    35701 gaccggcgat accactggtt atctggctaa tccggaggaa gtgtaaaatg ggaaatggat
    35761 ggcatgaatg gccactggtt atctttaccg tattgggtca atgcgtagta ggcgcgctga
    35821 ttgttagcgg aattggctgg ttcgccgcca aaaatgacgc agaccgtcag cacattattc
    35881 gtggcatgtt tttcctctgg ctgttgatgg gcatcgggtt tattgcctct gtcatgcacc
    35941 tgggatcgcc actgcgggcg tttaactcgc tgaatagaat cggagcttct ggtctgagta
    36001 acgagattgc tgccggttcc atattttttg ccgttggcgg attatggtgg ctggtggcgg
    36061 tcatcggtaa aatgccgcaa gcgttgggga aactctggtt acttgtcagc atggcactcg
    36121 gcatcatttt cgtctggatg atgacctgcg tgtatcaaat cgacactgtg ccaacctggc
    36181 ataacggtta cactacgctg gctttcttcc tgacggtatt gttgagcggt ccaatacttg
    36241 ctgccacaat tctgcgtgca gcccgcgtta catttaatac cacaccattt gccatcatta
    36301 gcattctggc gctgatcgct tgtgccgggt tgattgtgct gcaaggtttg tctctggcgt
    36361 ctattcactc atccgtacaa caagccagcg cactggtacc agattacgcc tccttacagg
    36421 tctggcgtgt ggtattgtta tgcgcaggtc ttggatgctg gctttgtccg ctcattcgtc
    36481 gccgtgaacc tcacgttgcc ggacttgttc tggggctaat cctcattctt ggcggtgaga
    36541 tgatcgggcg tgtgctcttt tatggcctgc acatgacggt aggaatggct attgcaggtt
    36601 aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt aggaatgacc catttttcac
    36661 agcaagataa tttttctgtt gcggcgcgcg tgttgggcgc gctgttttat tacgctccag
    36721 agagtgcgga agccgcacct ctggttgcgg tactcaccaa tgacggctgg gaaactcagt
    36781 ggcctctacc agaagcgtca ttagcgccgc tggtgacggc ttttcagaca caatgtgaag
    36841 agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc
    36901 cgtggggatc ggtctggctg gatcgcgaat ctgtgctgtt tggcgattca acattggcac
    36961 ttcgtcagtg gatgcgcgag aaaggcattc agttcgaaat gaagcaaaac gaaccggaag
    37021 ataattttgg ttcactgctg ctgatggctg cgtggttggc ggagaatggt cgccagacag
    37081 aatgcgaaga attgctggca tggcaccttt ttccgtggtc aacacgtttt cttgacgttt
    37141 ttatcgaaaa agcagaacac cctttctacc gtgcactggg tgaactcgct cgcttaacgc
    37201 tggcgcaatg gcaatcacaa ctgttgattc ctgtcgcggt taaaccgctg tttcgatagg
    37261 atcaccgtat tattgccgga tggtgacgat tacacgcaat tcggcaatta ataggttaca
    37321 gtgtcacgtt ttttttatct cttaaagcac acactgcttt tgcggctggc ctcttttgcc
    37381 gcaaaatagt cgcccgtgtt tcattgccca tttctgctca tgcatcatct acacatctat
    37441 ccggatctgc gcactatgtt ccgccgtctg cttatcgcaa caatcgtcgg tattcttgcg
    37501 gcctttgccg ttgccgggtt tcgtcatgcg atgctgctac tggagtggct gttcctcaat
    37561 aatgactctg gcagtctggt caatgcagcg acaaatcttt ccccgtggcg acggctgcta
    37621 accccggcgc tcggcggact ggcggcgggt ttgttgctga tgggctggca gaaatttacc
    37681 cagcaacgcc ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga tggtcagttt
    37741 gattacgcag caagtctggt taaatcgctt gcctctctgc tggtagtaac cagcggcagt
    37801 gcaattggtc gcgaaggtgc gatgattctt ttagctgccc ttgcagcctc gtgttttgcc
    37861 caacgtttta cgccacgcca ggagtggaaa ttatggatcg cctgtggagc tgcggcggga
    37921 atggctgcgg cctatcgcgc cccgcttgct ggtagtttat ttatagccga agtgctgttt
    37981 ggcactatga tgttggcctc tctcggcccg gtgattattt ccgccgtcgt ggcgttgctg
    38041 gttagcaatc ttattaatca tagcgacgca ttactctaca gcgtacaact ttcagtgacg
    38101 gttcaggctc gtgactatgc gctgattatc agtacaggtg tgctggcagg tctgtgcgga
    38161 ccactgttgt taacgttaat gaacgcctgt catcgtggat ttgtgagtct caagcttgcg
    38221 ccgccctggc aactggcact gggcgggttg atcgtgggtc ttctttccct gttcacacct
    38281 gcagtgtggg gcaacggcta tagcaccgta caatcctttt taaccgctcc gccactgtta
    38341 atgatcattg ccgggatctt cctctgtaaa ctgtgtgccg tgctggcgag tagtggttct
    38401 ggcgcgcccg gtggtgtctt tacaccgacg ctatttatcg gtcttgccat tggcatgttg
    38461 tatggtcgta gcctgggatt atggttacct gatggcgaag aaattacgct tttactcgga
    38521 ttgaccggga tggcgacact gttggcggcg accacgcacg cgccgactat gtcgacgttg
    38581 atgatatgtg aaatgaccgg ggagtatcag ctactccccg gtttattgat tgcctgcgta
    38641 attgcgtcgg taatttcgcg gacgttacac cgtgactcta tctaccgcca gcacactgcg
    38701 aagcatagct aagcggatgt attgccccag ttcacgctgt tcggcgcgcg gcagataagg
    38761 cagttcacca atgagcggtg ccggaagttt tttaccgagc acatcaatga tttccgcata
    38821 atgcgccagt cctgggttga ttcggttagc cacccagcca atgagcggca gcccgtcgtt
    38881 ggcaatcgcc tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa taccgacaac
    38941 catcaacacc ggcagttgtt cctgcaccac ccattcagag agcggacgca aatcattcat
    39001 cagactgcgc cagccgccag tcccttctac cacgacatga tcgactttat cggtcaggtt
    39061 tgccaggccg tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca cgctactttc
    39121 ttcttcgctt aacgcgatag gattaactgc ttcataaggc agttcgatgg ttgaaacact
    39181 ctgcaacacc agggcatctt tattacgcag cccttcgggt gtctctttgc tccccttcgc
    39241 tacgggttta tatcccgcaa ccgtttttcc ctgggaggct aacgcttgta gcaatgcgcg
    39301 ggaaaccacc gttttcccta cagaagtgtc tgtaccggta ataaagaaac gcttcagcat
    39361 cactaactcc accgttatgc ttcacaaata taaaccagga aaataattaa ccttgaaagt
    39421 ctaagttatg ctttcctggc ccaaattgag atagcgcaaa ttttggtaga acagttaaaa
    39481 aatgttaacc ctgcaacaga cgaatcaaca aagaaccgtt atacatcgcg tcttttacca
    39541 gcgcagcgcc tgccatcgtg ccctggttag aaaactgagt actctcaacg ctaatgtgct
    39601 gactatacgc aggaagggcc tgctgacgga tgctgtccga gatgaccggg aagaggatat
    39661 ctgccgcttt acttaacggt gagccaatca gtattttttg tgggttaaat aaattcacca
    39721 tgatggcaag aatgcgaccg acatgcgcgc ctaccccggt aatgatgtct tttgccagta
    39781 aatcgccgcg caatgccgcc tgacacaatg agtccacggt taacggttgt ccatgtaaca
    39841 tcgagctcat ggattgattg agacgcagct gtgccagctc aagaatactg tccacactgg
    39901 cgatggtttc gaggcaaccg tgattcccgc aataacagcg tttcccatac gggtcgacct
    39961 gtgtgtggcc tatttccacg aggctactgc tgcctgcgtg tagcagatga ccatcggtaa
    40021 tgacgccagc ccccacgttg tgatcgataa ccacctgaat cacatcgcgc gccccgcgtg
    40081 aggcaccaaa caaggcctca gccatcgtcc atgcgctgat atcatgctga atataaaccg
    40141 gtacgccggt atgctgctcc agcgcctcgc cgagcggcat ctcttttaca tcctcgtaga
    40201 acggcatgcg atgtacaata ccattttccg tatcaataat tcccggcaag gttatggcaa
    40261 tcgaagtcag acgctcaagt tttttctggt ggcggataaa aaactgatcg atatgggaaa
    40321 tgatacgatc cagcaatggc gagtcatctt ttaacgccag ttcctgcgac tcctccacta
    40381 ccagtttgct gctcagatcg cgcaaagcaa ggaaaatctc cccgcgacta atgcgcagag
    40441 aaagatagtg ccaggcttca gtttcaacca ccagccccac tgccggacgg ccacggttcc
    40501 ccgcttcttt gatttccagc tcttgcacca ggtgtgcttc gagcatctca cggacaattt
    40561 tagtgatact ggcaggagcc agttgcgcca gacgggaaag atcgatacgc gagactggac
    40621 caagctgatc aatcaggcga taaactgcgc ccgcgttggt ttgctttatt tgatcaatgt
    40681 gcccaggctg gttttcagca accaccgcat actccctata ttttcgcgct ccgaaataat
    40741 ctgtaggcta tggtgaagca cttcaatacg tgtcgtcaaa tttttactta ggcatgtgat
    40801 taacagcaca tttttcgggc tttttcgctg aaatttccct cacctgagag cattcagcag
    40861 atgaatacga aagttacgcg cagccggact ttgttcatga tgtttcggcc agaccaacca
    40921 catttcagaa accgcatcct cttcagcaat cggcacccag cgcatttcgt tgagctgaac
    40981 acgtttaaat gacgcaggca aaattgaaac acccagacca gcggaaacca gaccgatgat
    41041 ggtcattgcc tcacccacct cctgagtgat gacgggcgtc aaattgtaac gtctcatcag
    41101 cccgagaata tcgtcataca gccctgttcc gacgtgcgga tcaaaaaaga caaagggttc
    41161 tttcgccagt tcagccagcg ttacactcgg gttatttgcc aggggatgat cgtgcgggat
    41221 catcgccata agcggttcat ggacgatgac tgcgtgttca agcgtctccg gtaacgctgt
    41281 gttacgcagc aatcccatat ccagcgttcc ttcaatgagc ggagcgattt gctcgcgagt
    41341 gttcatttcg cgggtttgta aatggacatc gggataatca cggcgaaaca gcgataacgt
    41401 atcggacacc gcccgaataa aaggagcgga cgaagtaaaa ccaatgcgca actcccccgc
    41461 ttcgccctga tgcagccttt cagcgcgagc ggcagcatca tccaccatag acaggatttg
    41521 ccgactatct acaagaaact gttttcctgc ttccgtcagc aatacactgc gattggtccg
    41581 tgccagcagt cgggcaccaa tttgttgctc cagcgcctga atctgctgac ttagcggtgg
    41641 ttgcgaaata ttcaggcggg cagcggcgcg cccgaaatgc agctcttctg caacagcaac
    41701 aaagtaacgc agatgacgaa gttcaatatt catatttaaa acatcttatt tgagattatt
    41761 aatatattag acagaacaat tcgattttcc taatctattc ataagcctga tccacaggca
    41821 tatttagcaa ggatttcaag tgagccgtac tacaactgtt gatggcgctc cggcaagcga
    41881 cactgacaag caaagcattt ctcagccaaa tcattttatt aaacgcggta cgccgcaatt
    41941 tatgcgcgtc accctggcgc ttttctctgc cggactggca accttcgcac ttctctattg
    42001 tgtgcaacct atccttccgg tgctttcgca ggagtttggt ttaacgcctg cgaacagtag
    42061 tatttcactg tccatttcaa cggcgatgtt ggctatcggt ttgctgttta ctggcccgct
    42121 atccgatgcc attggtcgca aaccagtgat ggtcacggcg ctactgttgg cctccatttg
    42181 tacgttactt tcgacaatga tgaccagctg gcacggcatt ttgattatgc gcgccttgat
    42241 tgggctttcg ttaagtggcg tggcagccgt tggcatgact tatcttagcg aggaaatcca
    42301 ccctagtttc gtggcctttt caatggggtt gtatatcagc ggcaactcaa ttggcggcat
    42361 gagcggacgc ttaattagcg gtgtcttcac ggactttttt aactggcgaa ttgctctggc
    42421 ggcaatcggt tgtttcgcgc tggcctcggc gttgatgttc tggaaaatcc tccctgaatc
    42481 acgccatttt cgcccgactt cgctgcgccc taagacgtta tttatcaact ttcgtctgca
    42541 ctggcgtgac cggggattac cgttattgtt cgcagaaggc tttttgctga tggggtcgtt
    42601 cgtcacgctg tttaattaca tcggctatag gttgatgctt tccccctggc atgtcagtca
    42661 ggccgtggtt ggcttattat cgctggctta tttgaccggt acatggagct cacccaaagc
    42721 cggaaccatg accgcccgct atggacgcgg tccggtgatg ttgttttcga cgggggttat
    42781 gctgtttggt ttactgatga ccttattcag ctcgctgtgg ctgatctttg ccggaatgtt
    42841 actcttctca gccggattct tcgcagccca ctccgttgcc agcagctgga tcggcccccg
    42901 cgcaaaacgc gctaaaggcc aggcctcctc gctgtatctg ttcagttact atctggggtc
    42961 gagtattgcc gggacgctgg gtggtgtttt ctggcataac tatggctgga acggcgtcgg
    43021 cgcatttatt gctctgatgc tggtcattgc tctgctggtc gggacgcgtt tgcatcatcg
    43081 tctgcacgcc tgaaaaaata agtccggact gaggtaaata cccgtccgga cttattgcct
    43141 gctcaaacca acgttaatag ccatcctaaa atagacgaag cgccagccaa ttcccgcagc
    43201 gcatctagcg tcatcaggat tataagtacc caaataaacg gattcatttt gctgtgtgtc
    43261 atttattact gatgcgcagt tattctaccg ctttgtaagt agtaaaatag ttaacacgat
    43321 caagactact ttattggtag ctaaattttc cttaagtcac aatacgttat tatcaatgct
    43381 gtaatttatt cagcgtttgt acatatcgtt acacgctgaa accaaccact cacggaagtc
    43441 tgccattccc agggatatag ttatttcaac ggccccgcag tggggttaaa tgaaaaaaca
    43501 aattgagggt atgacaatga aaaaagtatt agctctggtt gttgccgctg ctatgggtct
    43561 gtcttctgct gcctttgctg cagagactgc gaccacacct gctccgactg cgacgaccac
    43621 caaagcagcg cctgcgaaaa ccacacatca taaaaaacag cataaagcag cacctgccca
    43681 gaaagcgcag gcggctaaaa agcatcataa aaatgccaaa gctgaacaga aagctcctga
    43741 acaaaaagcg caggcagcga agaaacacgc caagaaacac agccatcagc aaccggcaaa
    43801 acctgctgca caacccgcag cgtaagtttt caacagtaat gctggcgcgc cccctcgcgc
    43861 ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga gggtagtatg
    43921 gtgggccgtt atcgctttga gttcattctt atcatcctta ttttatgcgc actgattgcc
    43981 gcccgttttt atctttcctg atcgtagtta tctgatttta ctcccacttt catcccgtcc
    44041 cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc agagtgtgat
    44101 atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc ttttgtgttt
    44161 gcagataaac cagacgttgc caaatcggca aacgatgagg tcagcaccct gttttttggt
    44221 catgatgatc gtgtgccagt gaatgacacg acccaatcac cgtgggatgc ggttggccaa
    44281 ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa tctggcatta
    44341 acggcaggac actgtttatt gacgccgcca aagggtaaag ccgataaagc ggtagcgctg
    44401 cgtttcgtgt caaataaagg tctttggcgc tatgagatcc acgacataga aggccgcgtt
    44461 gatccaacac tgggaaagcg gttaaaagca gatggggatg gctggattgt acctcccgca
    44521 gctgcgccgt gggacttcgg tttgattgtg ctacgtaatc ccccttctgg cattacaccg
    44581 ttgccgttat ttgagggaga taaagccgcg cttaccgccg cattaaaagc ggctggtcgt
    44641 aaagtgactc aggcgggcta ccctgaagat catctcgata cgttgtacag tcatcaaaac
    44701 tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcgc atcagtgcga taccttgccg
    44761 ggtgacagcg gttcgcctct gatgttgcat accgatgacg gctggcaatt aattggggtg
    44821 caaagctcgg ctcctgccgc gaaagatcgc tggcgcgccg ataaccgggc catttctgtt
    44881 accggttttc gcgacaagct ggatcaactg tcgcaaaaat aatgttcaaa cgctgcccga
    44941 cagcgcgggc agcgtgttca tcaggcaagt ttcaccatga tcattccagc caacagcagc
    45001 accaggccaa tccagccttt acgatttaac cgttgaccaa acaagatcca acctgcggct
    45061 aacgtggcgg caataccaaa cccgccccac aatgcataag cgacagacaa gtcgatccct
    45121 ttaacggcct gagaaagcgc actaaaggca gccagcaccg ccgccagaga gagcaagcca
    45181 aatattttgc gacgaaagcc gtcagaaaat ttcaaaaaga cgttagcaac gatttccagc
    45241 acgattgcca atgccagcca ggcggcgtga acccattcaa actgcgccat ggttcacctc
    45301 cagttcaggt ttacgcgctt tacgggtacc tgacttgatc aacacaatcc cggccaccag
    45361 ggtggttaac ccggcaattt tcatcagcga taaactttcg tcgaataaca aaacgctaaa
    45421 caaggtaata aataaaatac caataccttc ccacagcgca taagctacgc ctaaggcgat
    45481 ttttttaacg gcgaaagaga gaaatatata cgacagagaa atcatcacca gcattaaaat
    45541 aaagccgcca tttccctcac tgacgctcgc ccatttcatt gacagcgtac cggtaatttc
    45601 cgtagcaata gccaggccta ataaaatcca ataaatatac attgtccttc tcctgcaaga
    45661 gaattatttt aattttcgct taattcagcg aaaccaaagt aaagtgatca agccccgcgc
    45721 atagcgcaag cttaggcaga agaaaggact aaagcgcgtt gcgccagtgc tgctcaccta
    45781 cgagcaagat agtggatgag gtacgaagat gggtaaatgt agaaaataac gtcctgaaca
    45841 aattgtccat aatattacaa ttatccgcag tgttgcttct cgtcatcgcg gatgataatt
    45901 gtcctcggta gttgaacacg cctgatttgt atcatagctt aagaattaac tcaaaatatt
    45961 ttcacttctt tacctgagcg gtttgatttt cgttatgatg aaggagcgaa aaagacatta
    46021 ttattagcaa aggaagaaaa aacggggaca agcatggcaa agccgatcat cacgctcaat
    46081 ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg tatccgtttt
    46141 gccgccgaga ttatcgtgcc gtttattctc gcattattta ttgctgttat tcttaacccg
    46201 ctggtgcaac acatggtccg ctggcgtgtg ccgcgtgtac tggcagtgtc gattttgatg
    46261 accatcatcg tgatggcgat ggtgttgctg ttagcttatc tggggtccgc gctcaatgag
    46321 ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca agcgattgaa
    46381 ccgttgttgc aacgcgtagg gattgatgtc tcagttgacc aactggcgca ttacattgat
    46441 ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa tgccatgtca
    46501 tcaatctttt tattgctgct gacggtgctg tttatgttgc tcgaagtgcc acaattgccc
    46561 ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat tcagcgcgcg
    46621 attgacagcg tgtctcatta tctggtgctg aaaacagcca tcagcatcat caccggcctg
    46681 gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg gggattgctg
    46741 gcctttgcgc ttaattacat ccccaatatt ggttcagttc tcgcggcaat cccccctatc
    46801 gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc gggatatctg
    46861 ctgattaatc ttgtcttcgg caatattctg gagccgcgca tcatggggcg tgggctgggg
    46921 ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt aggaccggtg
    46981 ggtatgctgc tttccgtgcc gttgacaatt attgtcaaaa ttgcgcttga acaaacagcg
    47041 ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg gcctccgcag
    47101 aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa
    47161 cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat agccagtgtt
    47221 catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca caggcatacc
    47281 agcaatcgga ctcttcggat catcctgcgc cgccgggtta accgtatcgt tagcaccaat
    47341 caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt ccagcacgat
    47401 gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca aacgccccgc
    47461 aaccgggtgg ataccgaaac gcacgttaat accacgagcg cgcaatttct cagtaatttc
    47521 agcgacagga tattgcgcct gcgcaactgc catgccgtac cccggagtaa tgatcactga
    47581 atgggaattt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac
    47641 ttcctgatca tcaccagtag aagagccgtc ggtgccgaaa ccgcccgcaa taacgctgat
    47701 aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcccccg aagaaccgac
    47761 cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc
    47821 ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac caccgatgga
    47881 ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca atgccagcac
    47941 ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg aaacgatcag
    48001 agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg aaatcttgcc
    48061 acacagtttg ccgaacgcca ccaccgaacc cgtaaacgtt accgccccga tgaagatacc
    48121 gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg cgtcatgatg
    48181 cagatagctg ttaaaaccaa ccagcactgc cgccagaccc acgaagctat gcaggatcgc
    48241 caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac caattgcccc
    48301 accaataacc atcgccagca agatccagcc aacattaccc gtatccggtc caaaaatggt
    48361 tgcgattaac gcaatcgcca taccggcgat accgaaattg ttaccctggc gagacgtttc
    48421 atgtttcgaa agcccggcca gactgaagat aaacaggatc gcggcaacaa tgtatgcagc
    48481 tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt ttcagcatgc
    48541 gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc gcgataaaac
    48601 taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca acaataatcc
    48661 ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcgtgcgat acattccaca
    48721 ccacgtaata accgacaacg caggccagcg cgaacacagt gaagtgtcca aggaactctt
    48781 ttggcgcaac gcttgccatc cagccgaaaa ggatgattgc caacgccatc aacgcgtatt
    48841 tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc gctttttgtg
    48901 ccgtctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac
    48961 ggatcacagt cacgccacga atcaccacat catcaaaatc cacagtgatg ttgccgtctt
    49021 tctctttgca caacagtttc agcagattaa cgaggttagt gccgtaaagc tgtgaggatt
    49081 gcgtcggcag acggcccgga agatcggtgt aaccaattac tttgacacca ttttccgtag
    49141 tgaagatttc acccggcacg gtgtattcac agttgccgcc gttttgggct gccaggtcga
    49201 caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc ttcggcgctg
    49261 gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa
    49321 agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag ccatcgccgc
    49381 tgcccgcttc ctctttaaaa tccagctcga ggaattctgc gcccatactt tgaacttgtt
    49441 ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc
    49501 caatggcggc cagacctgcg acaccggcac caatcaccat cacttttgcc ggtggcactt
    49561 tcccggccgc agtgatttgt ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa
    49621 tggcgcgata accggcgata ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg
    49681 agatacgcgg cacagagtcc atcgccatca cggtcacgtt acgttccgca agtttttgca
    49741 ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca ggattcagta
    49801 acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac tgccagacgc
    49861 tattcccttc tacaatttca gcgcccgctt gcacaaacgc cttatcgtca aaacttgcca
    49921 gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc tgttccactg
    49981 tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt attccaattc
    50041 gcatgatatt cccttccatc ggttttattg atgatggttt gcttgtgtca ggagccacac
    50101 aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa caaacgccct
    50161 ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt cggcgtgaac
    50221 tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa cgccggataa
    50281 agactgataa ttgtcttcgg cggccgggta aaacgagaca tcgccccggc acaaatcact
    50341 acttaacatt aaattaactt atacaattca gttgcttcag tagtattgat ggtgatacgg
    50401 ctgtttttta agcatagacg gtcatttgag cagaattaaa attggtttaa ggaatgtgat
    50461 atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac gtttctcgtt
    50521 aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga
    50581 acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac gcagcaacag
    50641 aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg gttaccggtc
    50701 gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg
    50761 ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac tggcgtgtgg
    50821 tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta
    50881 acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt gacacggtca
    50941 ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc aaagcggcaa
    51001 aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac cagggcggta
    51061 accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc gtccagagcc
    51121 cggatgtaat cccggcagat tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag
    51181 ccgcgaagaa agttgagatc ccgggtgttg cgactaccgc atcaccaagt tctgaagtcg
    51241 gtcgcttctt tgaaacccag tcatcaaaag gcgggcgtta caccgtcacg ctcccggatg
    51301 gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg ttcgacagca
    51361 tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg
    51421 ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa cgtggtaata
    51481 acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat aaggcggctt
    51541 gatgccgcct ttcgcatttt tagtcataaa tttccctgcg accattgcat gcagacacat
    51601 ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt tgccctaata
    51661 tttattcttc ttgtcacgtt ttaatcactg gatatcgatg gaaaagaaac tgggactgag
    51721 cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca gtctgccgca
    51781 aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta ttactggcgc
    51841 tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgcc ccgaacttga
    51901 cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct tttgttccgc
    51961 atggggatac tggctgtgcg cggtcatcgc caacgtctcc tatctggtga tcgttttttc
    52021 cgcgttaagc ttttttacgg acacgccgga attgcgcctg tttggcgatg gtaatacctg
    52081 gcaatcgatc gtcggtgcat cggctttatt gtggattgtt cactttttga tcctgcgcgg
    52141 ggtgcaaacc gctgccagca ttaaccttgt ggcgacactg gcaaaattgt tgccgttggg
    52201 tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc tcgacttcac
    52261 cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc tgatcaccct
    52321 gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc gtaataagcg
    52381 tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt atttgctggt
    52441 aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc gtaacccgtc
    52501 aatggccggt ctgatggtgg aaatgatggg accgtggggc gaaatcatca tcgctgccgg
    52561 tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcag cagaagttcc
    52621 gttccttgca gccactcata aagcattccc gcgcattttt gcgcgccaga acgcacaagc
    52681 tgcgccatcc gcttcactgt ggctgaccaa catctgcgtg caaatttgtc tggtgctcat
    52741 ctggctgacc ggttccgatt acaacacatt gctgaccatc gcctcagaaa tgattctggt
    52801 gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac tgcataaggc
    52861 ggtaggtgtc ggtgcctgca tttatggctt atggttactg tacgcctctg gtccaatgca
    52921 cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt acgcgcgtaa
    52981 aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt
    53041 aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc atcgttggat
    53101 agcaaaggag ataacgatgg gtaaaaccca gtccttgcca atattaatta ctggcggagg
    53161 tcgccgcatc ggcctcgccc tcgcatggca tttcattaat caaaagcaac cggtgattgt
    53221 cagctatcgg acgcactatc ccgccattga tggactgatt aaggcaggtg cgcagtgtat
    53281 tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag tactaaaaag
    53341 cacccatggt ctgcgtgcta ttttgcataa cgccagcgcg tggatggcgg aaaaaccggg
    53401 agcgccactg accgacgtac tggcttgcat gatgcagatc cacgttaata ccccatacct
    53461 gctcaaccat gctctggaaa gattactgcg tgggcacgga cacgccgcca gcgatatcat
    53521 tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catattgcct atgctgcaag
    53581 caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac cggaagtgaa
    53641 agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg ccgaatatcg
    53701 acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga
    53761 cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtctcc cacttgatgg
    53821 cggtcgtcat ctgcgttaat gcagcttaat ccagcatata atcaacaccc atgcgcttat
    53881 tccccagcac gcaacagagc caacaaaagc agcgggcaag cgcatcattc cggtgaaata
    53941 ccatagcgac gccaggtaaa caaaataggg aataatcgac cacatactaa aaatgatggt
    54001 tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa
    54061 ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg ctaaaacacc
    54121 aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca tctttttacc
    54181 ttaacacttc cataacaagt catcagtaga atacctgatg aaaccttgtt tagaaacgat
    54241 tgatagtaag taaaaacagt gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta
    54301 tgaacactat cgtatttgtg gaagatgatg cggaagtcgg tgcactgatt gccgcatacc
    54361 tggcaaaaca tgatatgcag gttaccgtag agccgcgcgg cgaccgggcc gaagaaacta
    54421 ttctgcgaga aaatccggat ttggtgttac tcgacatcat gctaccaggc aaggacggca
    54481 tgaccatttg tcgtgattta cgcgcaaagt ggtccggacc gattgttctt ctaacctctc
    54541 tcgatagcga tatgaaccac atcctggcac tggaaatggg cgcctgcgac tatattctca
    54601 aaacgacgcc ccctgctgtt ttgttagcgc gtttacgctt gcatttgcgt cagaatgagc
    54661 aagccacagt gaccaaaggt attcaggaaa cgtctctgac tccctacaaa gccctgcatt
    54721 tcggcacgtt gaccatcgat cccatcaacc gcgtagtctc cctggctaac actgaaatct
    54781 cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc gggcaaatca
    54841 tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacggg ctggatcgta
    54901 gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc gcagaacctt
    54961 atcgtattaa aaccgtgcgt aacaaaggct atctttttgc gcctcatgcg tgggaataag
    55021 cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc cttgtgatgt
    55081 ctctgctggt cgggctggtg tacaaattta ccgccgaacg cgcaggcaaa cagtcgctgg
    55141 atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag atccccccac
    55201 acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat ctgcgtgtcg
    55261 agccgctgag taaatatcat cttgatgata tttccatgca ccggctgcgt ggcggcgaaa
    55321 ttgtcgctct ggacgatcag tacacttttt tgcaacgtat cccacgcagc cactacgtgc
    55381 tggcagttgg tcctgttcct tatctttatt acctccatca gatgcgattg ctggatatcg
    55441 ccctgatcgc ttttattgcc atttccctcg cctttccggt gtttatctgg atgcgtccgc
    55501 actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat gggcatctca
    55561 gtgagcgtat ccactttgat gaaggttcga gctttgaacg acttggcgtc gcatttaacc
    55621 agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt gacggtatcg
    55681 ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg agcgataacc
    55741 tgagcgccgc cgaatcccag gcgttgaatc gcgatatcag tcaacttgaa gctttaattg
    55801 aagagctgct gacttatgcc cggctcgatc gcccacaaaa tgagcttcat cttagcgaac
    55861 cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta acgcccgata
    55921 aaacggtacg gataaaaacg ctcgcgcaag gccattatgc tgcgttggat atgcgcttaa
    55981 tggagcgcgt gctggataat ttgctcaata acgccctgcg ctactgtcac tcaacggttg
    56041 aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat gatggcccag
    56101 ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc gatcccagcc
    56161 gggatcgctc aaccggcggc tgcgggctgg ggctggccat tgtccactct atagcactgg
    56221 caatgggcgg tacggttaat tgtgacacca gcgaactggg gggtgcccgc ttctcgttca
    56281 gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactat gcgcacggtg
    56341 gtcaagtcac caccactgtg ctataaaata aggatgttgt aactaaagtg attaatatta
    56401 tggcgcgtta cgatctcgta gaccgactca acactacttt tcgccagatg gaacaagagc
    56461 tggctgcatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc gtgttctctt
    56521 tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaaacaac
    56581 atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta tttattcaac
    56641 aacagtccga aaatcgcagc agcaaagccg ccgtccgtct gcctggcgtg ttgtgttacc
    56701 aggtcgataa cttttcgcaa gcagcgttgg tcagtcatat tcagcacatc aataaactca
    56761 agaccacgtt cgagcatatc gtcacggttg aatcagaact cccctccgtg gcacgttttg
    56821 aatgggtaca tcgtcatttt ccggggctga ttacccttaa tgcttaccgc acgctcaccg
    56881 ttctgcacga ccccgccact ttacgttttg gctgggctaa taaacatatc attaaaaatt
    56941 tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca cgcagtgtcg
    57001 caccgtggac gcgcgaggag tggcaaagaa aactggagcg agaatatcag gatatcgctg
    57061 ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc
    57121 gcgtctggta taagggagat caaaaacaag tacaacacgc ctgccctacg ccgctgattg
    57181 cgctgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg ttaaattacg
    57241 atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt ttaatcattc
    57301 cacggctgca cctgtatgtt gcagattaac ccccggcttt catactgccg accatctgtt
    57361 ctggccgtac ccagcggtca aactcggctt cgctaagata ccccagcgca agggccgccg
    57421 cttttaaggt cagcccttct ttatgcgcct ttttggcgat ctcggcggct ttgtcataac
    57481 caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat tgattgattc
    57541 gctcacgatt cggttcaatg cccactgcgc agtgtttgtt gaaactttcc atgccatctg
    57601 ccagcaagcg caccgattgc aggaaattat ggatcaccat cggacggaag acgttcagtt
    57661 caaagttacc ggaagcgcca cccatgttga tcgccacgtc attccccatc acctgacagc
    57721 agagcatggt taatgcttca cactgtgttg ggttcacttt ccctggcatg attgagctgc
    57781 ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca gaggccagcc
    57841 agcggacatc attggcaatt ttcatcagtg acgcagccaa ccctttcaac gcgccgtgcg
    57901 cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggtgcg gtaacaaacg
    57961 gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatattcc ggatgggtat
    58021 ttagtccagt acccaccgct gtaccgccca gagccagttc cgctacgtga ggcaggctgt
    58081 attcgatatg tttgagatta tgctccagca tcgctaccca gccggaaatc tcctgcccca
    58141 gcgttaacgg cgtggcgtcc tgcaagtggg tacgaccgat tttgacgata tcagcaaatg
    58201 cgcgggattt ttcactcagc gtctgggtca gggttttaag ttgtggaatg agttgcttgc
    58261 gcagcgccag tagtgccgca acgtgcatcg ccgtcggaaa gacatcgttg gaactttggc
    58321 ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg ccaccgagta
    58381 attcactggc ccggttagcc agcacttcgt tcatattcat gttactttgc gtgccggagc
    58441 cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt acttcatccg
    58501 ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct tcattaactt
    58561 ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc attttctccg
    58621 tcgaaatgcg gaaatgctcc agcgagcgct gagtttgtgc gccccacagc ttatctgccg
    58681 ggacatcaat cgctcccatc gaatcttttt cgctgcgtac tgtattcatg gcctgctcct
    58741 cacctgatta attttttctt tctgttttgc tttcgttaag caacttttag ctcactcatt
    58801 atttaccatt tgataacaaa cgtttggtct ttcgtgccat gtaaaaaaac cgccccgaag
    58861 ggcggctctg tttatttcac acaacgggtg cattgtgtga gttgtatctg ctggaagaag
    58921 tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc aattttccag
    58981 atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat acttccctgc
    59041 gccaatacag ctgccggacc accgatactg ccgaggtaga agccgccgtg ttttttacag
    59101 gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact tccgccctgc
    59161 gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc aagagaaccg
    59221 gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg atctttaata
    59281 tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg agcaatatca
    59341 cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata ctgcgacaac
    59401 tgtgcgagga tctctttcat cggacggttg aggtcaacgc gcaccgcttc gccttctcct
    59461 gctttgcgca gctcttccgg gatatatttg cctggattat gttccagttt ttcgatccag
    59521 atcccctgac ggttgatctt cgctttgata ttacggtcag cagaacagga gacgcccata
    59581 ccgaccgggc aggatgcgcc gtgacgtggc aggcgaatca cgcggatgtc atgagcgaag
    59641 tatttaccac caaactgcgc acccagacca agattttgcg cttcgatcag caattctttt
    59701 tccagttcca catcgcggaa cgcctgaccg tgctcattcc cttccgttgg cagttcatca
    59761 tagtatttag cggaagccag tttcaccgtt ttaagattcg tttctgcaga agttccacca
    59821 ataacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg catcttctca
    59881 accaggtaat ttttcagttt tcccggcgtc agtaacgctt tggtttcctg atagagatac
    59941 gtcttgtttg ccgaaccgcc acctttggcg atacagagga atttgtactc gtcgccatca
    60001 acggcataaa gatcgatctg cgctggcaag ttggtgccgg tattcacctc tttatacata
    60061 tccagcggcg cgttttgcga gtagcgcaga ttatcctcga tataagtgtt ataaacaccg
    60121 cgcgccagcg ctgcttcatc accgccaccg gtccatacac gctgcccttt tttaccaaca
    60181 ataatcgcgg tgccggtatc ctgacaggtt ggcagaacgc cttttgccgc gatgtcggag
    60241 ttacgcagga attgcagcgc cacatattta tcattttcgc tggcctccgg gtcacacaga
    60301 atgtcggcca cctgttgttg gtgagccgga cgcagcataa atgacgcatc atgaaacgcc
    60361 tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg cccttcaaat
    60421 tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc ttttttgagt
    60481 ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact tactgcctgg
    60541 tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa caatatccta
    60601 cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag gttacttccc
    60661 gtaggattct tgctttaata gcgggattaa tttccacatt aaaacaggga ttgatcatgc
    60721 aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg ttgactgaac
    60781 tttatggtat ggaaaatccg tccagccagc cgatggccga gttgtggatg ggcgcgcatc
    60841 caaagagcag ttcacgtgtg cacgatgccg caggagatat cgtttcactg cgtgatgtga
    60901 ttgagagtga taaagcgact cttttgggag atgccgttgc caaacgcttt ggcgagttgc
    60961 ctttcctgtt caaagtatta tgcgcagccc agccgctctc cattcaggtt catccaaaca
    61021 aacgcaattc tgaaatcggt tttgccaaag aaaatgctgc aggtattccg atggatgccg
    61081 ctgagcgtaa ctataaagat cctaatcaca agcctgagct ggttttcgcc ctgacccctt
    61141 tcctcgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctgctc cagccggtcg
    61201 caggtgcaca tccggcgatt gctcactttt tacaacagcc tgatgccgaa cgtttaagcg
    61261 aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt
    61321 taaaatcggc cctcgacagc cagcagggtg aaccatggca aacgattcgt ttaatttctg
    61381 aattttaccc ggaagacagc ggcctgttct ccccgctgtt gctgaatgtg gtgaaattga
    61441 atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg
    61501 cgctggaagt gatggcgaac tccgataacg tgctgcgtgc aggtctgacg cctaaataca
    61561 ttgatattcc ggaactggtt gccaacgtga agttcgaagc caaaccggct aacaagttgt
    61621 tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattcctgtg gatgattttg
    61681 ccttctcgtt gcatgacctt agtgataaag aaaccaccat cagccagcag agtgccgcca
    61741 ttttgttctg cgtcgaaggc gatgcaacgc tgtgtaaagg ttctcagcag ttacagctta
    61801 aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc aaaggccacg
    61861 gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt aacatctctt
    61921 gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga tttcatttta
    61981 tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc gctaggcgta
    62041 gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct cgaagacatg
    62101 gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct ggaagtcagt
    62161 tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt gaaacccatt
    62221 gccggaaaag taaatccgtg gattaaaagc ggtcagagcg tcatcttcaa cgaatcggtt
    62281 gatcatggtc ccttcccgct tgcccagctt aaaaaactga atctgatccc gtcgatggca
    62341 tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga tatggcaaaa
    62401 ggtgaaacgc cttttgagat taactcccgc attggttaca gcggagattc cagttccgac
    62461 atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt tagcggcggc
    62521 gagttccagt taaatgcaga cagagacggc aaagctatct ccctttccgg ggaggcgcaa
    62581 agtggtcgga tagacgcggt taacgaatac aaccagaaag tgcagttaac ctttaataat
    62641 ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg aaaccaaaaa
    62701 ctgtcgctgg aaaaaatgac catttcagtg gagggcaaag aactggcact gctggaaggc
    62761 atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa tagccaactg
    62821 gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagtgg caagctgact
    62881 ttaaaagtcg gccaaattga tggcgaagcc tggcatcagt ttagccagca atataacgcg
    62941 caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact ttatcaggag
    63001 aaagtgacgg aagccttctt tagcgctctg ccgctgatgc tgaaaggcga tccggtgatt
    63061 actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa tctgtcgctg
    63121 ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca ggaagtggac
    63181 cgctcggtta aatctctgga tgcgaaactg accattccgg tggatatggc aactgagttg
    63241 atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa actggcgaaa
    63301 cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac cttgcaggac
    63361 aataccatca ccaccagcct gcaatatgct aacggtcaga taacgttaaa cgggcagaaa
    63421 atgccactgg aagatttcgt gggtatgttt gcgatgccga cattaaacgt tccggctgta
    63481 cccgctattc cgcagcagta attcacagcc aaaacgccgg agatttttct ctccggcgtt
    63541 ttttcttact tcagcataaa gtcatactga atactcattt tcacatccca gtaattcagg
    63601 cgatcgccca gcgtactgcc atcgcgcttt tcccgtcctt tatcggcagc aagaaagaaa
    63661 ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccccctaa gatacccgct
    63721 ccttcaatcc gcacatctcg tttactggat tcatagccgc gggtcacaat cggtgcaaat
    63781 gtcagcgtaa attgcggagt taaacgatag ttgacgccca attgccagga ctgcattcct
    63841 tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca
    63901 ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc
    63961 caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt acgacttaaa
    64021 ccttcaagtt gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcaagc
    64081 ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg taccgatgcc
    64141 cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga
    64201 tgtttatcgt attttcccgt ctggctgtta taaacacctt catcacgaat accggttgtt
    64261 gaatcggtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggcgtt
    64321 aaagcccaat aacctaatgc tccttcagaa acagtcggta atgtacgttg cgaactgcca
    64381 gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc gaagcgacct
    64441 aattttagca ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa
    64501 cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc taatttataa
    64561 acataatacg ccccgccttc aacgccaata attattggaa taataaccac tattgaaatc
    64621 gagcaatcct ccctgtaccc atgcgtaaat atctcttccc gtgccagcac tgggcttatc
    64681 agccctgcgt aattcgtttt tcagtctgat gcgtagtccc gcttcatcgt cagccagttg
    64741 tgccgcgtaa gcagaggtaa ggccagaggc agccgtcagg caaataacgg ccatggccac
    64801 tatttttctc atagttgcac ctttgatgtt ctgattattt ttattgaata ttaactagtg
    64861 atatcgttga ttaattgctg ctgcactttt ttacgattat caatttcaac aacgatttct
    64921 ttgaattttt tatccgtgag cggataaaac cagataatga cgaatgccag tagcataaat
    64981 ccgcaaggta ctaaggcaat tgatgtgcgg atgcccgtaa taacttccgg cgtttgcgcc
    65041 tgattggcga tatatccgct taatcccaaa ataaaggcag gaattgaacc tccgattgcc
    65101 tgaccgcatt tacgggtaaa tgagaatagt gaataggtga gcccttcaat tcgcacgccg
    65161 gtcaggtatt caccgtattc tacggtatca gcttccagtg cccacatcac ggtcatggta
    65221 acgccctggc caattgatgc gatggccaac gcaaccaacg ccaccggcag tgaccagacg
    65281 gaaacccaga agaacagcag ataaccgcag gttcccagca aagcgccaat caggaaggta
    65341 ttctttttac cgatcctcgc gaccatcccc ggcaccagcg gtgccgatgc cacagtaccg
    65401 accaggtttt gcaccagtac cagcacagtg aacagcccgg tatcatttaa cacatagcgc
    65461 acgtagaaca acgacgaggc gctgaccgcg aaggtcgaaa tcagcacaca cagcgcaccg
    65521 atgcacaaca taaacagcgg gcgattccgt ttcagggttt gcagactgat cttcaatgac
    65581 ggctgcgcca cgatacgtac cacattctca cgcgtcgatt tgaagcagat gaagtaaagc
    65641 accattccgg caatcgccag cacaattgtc cagaaatgat ataccgacac catctcttcc
    65701 gggctggaat tcttaatgct cggtcctatc agaaatgcca ggcagacaaa ggtcaatgaa
    65761 gcgcaatccc gcgagccgcg cccagacggg cgcgggattg tggttgttgg gtcatcgcgg
    65821 tagcaagtga accataagga atattcacca ggctgtagca aagcccgagt cccatgtagg
    65881 tcaaatatgc atacacaact ttgctgctat ggctccagtc ggtcggcacc cagaatacca
    65941 gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg aattttcccc
    66001 atcgggtatt cacactgtcc accactcgtc cggcaaagac atcggcgaag gcatcgaata
    66061 cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg acgtcggtgt
    66121 agtaactcaa caggaagagc gcccccattg cgaaggcgaa gttattggcg acgtcaccga
    66181 ggctgtagcc gacgatggcg cgccaggaga gttgttgatt cattgtttgc ctccctgctg
    66241 cggtttttca ccgaagttca tgccggtcca gcgtttttgc agcagaaaag ccgccgactt
    66301 cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg
    66361 cgaggtcgca aaatcggcga aattccatac ctgctcaccg acgacagcgc tgacgcgatc
    66421 aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt
    66481 gtacattgag tgcaggccgg ctaacgtatc cacaccgtat tcggtgatga taatcggctg
    66541 atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa
    66601 atcgccgctt tggacatacc aaccgtaata acggttcagg cacagcacat caaagagatc
    66661 gctgatggta tcggtgtgag cgtcgcagaa cattacattg acacaggtga tcggacgcgt
    66721 cgggtcgagt ttacgcgttg cttccgccag tggcgcaaaa tattcccgtg caccttgcgg
    66781 acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg
    66841 cgcaatcagc tctttaatcg cctgtaagtg cgcctgctgg gtttccccgt tgactgcctc
    66901 ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt
    66961 aaagccgaca gctgcagttt catcaatcac cacgattcca tgttcatctg cccagtcgag
    67021 catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat
    67081 tagtgcgtga tcgtgcacca tcagcacgtt atcgaatcct ttgccgcgca aatccgcatc
    67141 ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttgatcagga actgttcgcc
    67201 cttcactgcc actgaccgga taccgacgcg cagcaggtag atatcacact ctgtctggct
    67261 tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt
    67321 cactacttgc aaagtcccgc tggtgccttg tccagttgca accacctgtt gatccgcatc
    67381 acgcagttca acgctgacat caccatttgc caccacctgc cagtcaacag atgcgtggtt
    67441 acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt
    67501 gtagagcatt acgctgcgat ggatcccggc gtagttaaag aaatcatgaa agtaagactg
    67561 ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag
    67621 ttcgttgttc acacaaacag tgatacgtac acttttcccg gcaataacat acggtgtgac
    67681 atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat tatttaccca
    67741 cactttgccg taatgagtga ccgcatcgaa acgcagtaca atacgctggc ccgcccaacc
    67801 tttcggtata aaaacttcgc gctgatacca gacgttgccc acataattac gaatatctgc
    67861 atcggcgaac tgatcgttaa aactgcccgg cacagcaatt gcccggcttt cttgtaacgc
    67921 gctttcccac caatgctgat caattccaca gttttcgcga tccagactga atgcccacag
    67981 gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggactc
    68041 ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa ttccgtgttg
    68101 cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga actgacaccg
    68161 cgatcaaaaa accaaaaact gcaataccca cagcgtgata ttgatcgcat taatctttaa
    68221 aacagcgttg acagaagcca caaaataagc aatcgaacgt tcattcgaac atattgggaa
    68281 cgataaaccc tcatgatata ttgctacgat gaatatttag gtggaattct ccgatccctg
    68341 ggaggatgat ttttgcacag agaaattacg atatgaaacg cgggctcagt tgtcaccagc
    68401 ccgcggaaag agagagagga tcaggatgcg gttaatatac cgccaatcat cgcgcgtaat
    68461 ccctgagcaa acgacgcttc acgcgccaga tcttccgctt cgatatcggc cagtacaccg
    68521 taggtcagat ccagcagtaa acgtgaagtc attgccgtgt tgatgtctgg gcttatctcg
    68581 cctttttgct gggcatcacg catccgctgg gcaacaaatt ccgtgatcgt catatgctta
    68641 tttttaagca tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcgaccacc
    68701 agcgctcgtt gcccaaaggc ttcatgggtg agagagacaa tagactcgac catatagtca
    68761 acgaaatgaa tcccttcaat cggttcccgg aaacgggcca gcgccctctc ctggtcctgt
    68821 aagataatcg cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccggga
    68881 ctaatggcgc aggatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa
    68941 aaaatctctc tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg
    69001 ttatccatca ttaaaccatt atagttagca tgaataacta gtttaccgcg ttgtcaggtg
    69061 agcgcctatc attgctgttg aatagtgcga aggcacactc aattcgtaag ctttttatcc
    69121 agatattggt taaccaatag ccgtgatttg gctgccagtt gtttagcaga gacgttgacg
    69181 agttgcacac gctatcccgg taaacaacac atacggctgt ttaccggttc gttagtgtat
    69241 taattgagtt cctgtacccc atcaccggag acggtgagaa tttgtccgct tacccagctg
    69301 gctgcaggcg aacaaaggaa cagcgccgcg ttagcaatat cttgcggttg gcccagacgt
    69361 ctgattggtg tgtgttgcaa cattttctgt tcaatttctg gggtaataac ggatttcagg
    69421 gcattggtta atattgcccc cggcgcaatg ccattcaccc gaatattttt ttcacccagg
    69481 tcaaacgcca tatttctgac cagatgactg gccgcagctt tagatgatgc ataggaggtc
    69541 atgtttatat ttttattttc tgccgccata gaagtaatgg tcagaataac gccaccgcca
    69601 tttttttcca tttctggcgc aataagttgt gacagatgga aaaaagaaaa tacattcagt
    69661 tcataagcgc ggcgaaaatc tgccattggc atatcaaacg gtttaggccc accgccaccg
    69721 gcgttgttaa ccaggatatc aactttaccc aacttactga cggcaaagtc tgccagtgca
    69781 gagagttcct gttcggaagt aatatcgcag cggcaggcaa atgcctgacc acccagttgt
    69841 tgaatttcat ctacaacatg attagctgca tcggcgttaa tatcactgac caccacagat
    69901 gcgccagctg tcgcgaatgt aatggcgatt tctttaccaa tacctgcacc cgcacctgta
    69961 atgatggcgc attttccgtc gagtctcagg ttgtcagaat taaacacata aacctcctgt
    70021 gaacttcagt aaaaataaaa ggcaccgcct gtgtgccaca ttttttatca ttaaccttga
    70081 ggaaaggaca ctaccaggta tagcagataa agagaggatt atttcgccgc aatgagccgg
    70141 gcgggaataa taagattgcg tgaatgcgtc tcttcatggg tgattttttg catcatgcga
    70201 tccgcaagtg tggtaccaag ttcccgcgct ggcgtgctgg cccagataac gggtatatca
    70261 tcaagtgtgg taggcgttgc atcggtaaat gccgccagcg aaacctgttg ctcaaagtaa
    70321 cgatcgactc cgctttcccc gctttgcctt cctgctttca gcaaaccaaa ccatgccccc
    70381 atcgcaatag tttcgttata gcaaaccacg gcgctgatgg tcgggttatg acgtaaaagc
    70441 gccgtgatag cttccgcggc ttgcttctgg ctggaagtgc actccagcac ccaatcgctg
    70501 tgaaacggca ggccaaattt tagtagagtc gcacaatagc cccccacccg ttctgcccgg
    70561 gttaatgagg aactttgccc tcccagccag gcgatacgct gatgcccatt gcgaatgaga
    70621 tgctccgtca acaactgtgc agcctgcatg ttgtccgggc gaaccgtatc aacgtcatca
    70681 agataactgg cacgggaagc gaaaatcacc gggatagctt tttcttctgc tatccgtcgc
    70741 aggtcatcgc tgcttcctgc agccccggca attaccacac catcgacacc ctgattcagt
    70801 aatagtgaaa accgctgtgc cagctgctcg ccgtctttac cgccgtgaag caaaaaaacc
    70861 atccgtccct gcgcttccag agcttccgtc aatccggccg tcaattcggc gtaaaacggc
    70921 gcagataaat cacggacgat caaaccaatg acgccacttt gcccgccgcg cagcgccgac
    70981 gcctgccgat tgcgcacaaa tcccagctct tcaatggcgg cgttaacgcg ttctcctgtg
    71041 gcggtagaga ttcgcccttt gccactaagc accagcgaaa cggtacttac cgacacgccc
    71101 gcagccagcg caacatcatg aatggttatt tttttggcgg tagccatgca gatgacctac
    71161 tccctgatta tgtgacagat aaaacgtttt accttttatt tatcttatac ccgctattat
    71221 cgttgcgtaa tgtgatttat gcctcactaa aatttgataa aacgttttat cttctcgcgc
    71281 aatttactga atccagattg ttctctacga ggagtcgttt tatgacggcg aaaacagcac
    71341 cgaaagtcac gctgtgggag ttcttccagc agttaggcaa aaccttcatg ttacccgtgg
    71401 cattattgtc gttctgcggc attatgctcg gcattggtag ttctcttagc agccatgatg
    71461 tcatcaccct gatcccggtc ctgggcaacc ccgtgttgca ggctatcttt acctggatga
    71521 gtaagattgg ctcgtttgct tttagtttcc tgccagtcat gttctgtatc gccatcccgc
    71581 tgggtctggc acgcgaaaac aaaggcgtag cggcattcgc tggcttcgtc ggttatgcgg
    71641 taatgaacct cgcggtaaac ttctggttga ccaataaagg cattctgcca accatggacg
    71701 ccgccgttct gaaagccaat aacatccaga gcattcttgg gatccagtcg atcgacaccg
    71761 ggatcctcgg tgcggtgatc gccggtatta tcgtctggat gctgcatgag cgattccata
    71821 atatccgcct gccggatgcg ctggcattct tcggcggtac gcgcttcgta ccaatcatct
    71881 cctcgctggt gatgggtctt gtcggcctgg tgattccatt agtctggccg attttcgcca
    71941 tgggtattag cggcttaggc catatgatca acagcgcggg tgatttcgga ccgatgctgt
    72001 ttggtaccgg tgaacgtctg ctgttgccgt ttggtctgca tcacattctg gtggcattaa
    72061 ttcgctttac cgacgcaggc ggcacgcagg aagtctgcgg tcaaaccgtc agcggtgcac
    72121 tgaccatctt ccaggcgcaa ttgagttgcc cgaccactca cggtttttct gaaagcgcca
    72181 cgcgtttcct ttcgcaaggt aaaatgcctg cgtttctcgg cggtctgcca ggtgcagcgt
    72241 tagctatgta tcactgtgcg cgcccggaaa atcgccataa aattaaaggt ctgctgattt
    72301 ctggcctgat cgcctgtgtc gttggcggca cgaccgaacc tctggaattc ctgttcctgt
    72361 tcgtagcgcc agttctgtat gtcatccacg cgctgttaac cggcctcggc ttcactgtca
    72421 tgtctgtgct cggcgtcacc atcggtaata ccgacggcaa tatcatcgac tttgtggtgt
    72481 tcggtatttt gcatggtctg tcaaccaagt ggtacatggt gccagtagtg gcggcaatct
    72541 ggtttgtcgt ttactacatc atcttccgtt tcgctatcac ccgcttcaat ctcaaaaccc
    72601 cggggcgcga tagtgaagtt gccagttcaa tcgaaaaagc cgttgccggt gcgccgggta
    72661 aatcaggtta caacgttccg gcaatcctcg aagccttagg cggtgccgac aatattgtca
    72721 gtctcgataa ctgcattacg cgtctgcgtt tgtctgtaaa agatatgtcg cttgttaatg
    72781 tgcaggcact gaaggacaat cgggcaattg gcgtagtaca acttaatcaa cataacctgc
    72841 aggttgttat cgggccacaa gttcagtcag taaaagatga aatggtcggt ctgatgcgta
    72901 ctgtccaggc ataaggataa gatatgttcg atttttcaaa ggtcgtggat cgtcatggca
    72961 catggtgtac gcagtgggat tatgtcgctg accgtttcgg cactgctgac ctgttaccgt
    73021 tcacgatttc agacatggat tttgccactg ccccctgcat tatcgaggcg ctgaatcagc
    73081 gcctgataca cggcgtgttt ggctacagcc gctggaaaaa cgatgagttt ctcgcggcta
    73141 ttgcccactg gttttccacc cagcattaca ccgccatcga tcctcagtcg gtggtgtatg
    73201 gcccttctgt tatctatatg gtttcagaac tgattcgtca gtggtctgaa acaggtgaag
    73261 gcgtggtgat ccacacaccc gcctatgacg cattttacaa ggccattgaa ggtaaccagc
    73321 gcacagtaat gcccgttgct ttagagaagc aggctgacgg ctggttttgc gatatgggca
    73381 agctggaagc cgtgctggcg aaaccagaat gtaaaattat gctcctgtgt agcccacaga
    73441 atcctaccgg gaaagtgtgg acgtgcgatg agctggagat catggctgac ctgtgcgagc
    73501 gtcatggtgt gcgggttatt tccgatgaaa tccatatgga tatggtttgg ggcgagcagc
    73561 cgcatattcc ctggagtaat gtggcgcgcg gagactgggc gttgctaacg tcgggatcaa
    73621 aaagtttcaa tattcccgcc ctgaccggtg cttacgggat tatagaaaat agcagtagcc
    73681 gcgatgccta tttatcggca ctgaaaggac gtgatgggct ttcttcccct tcggtactgg
    73741 cgttaactgc tcatatcgcc gcctatcagc aaggcgcgcc atggctggat gccttacgcg
    73801 tctatctgaa agataacctg acgtatatcg cagataaaat gaacgctgcg tttcctgaac
    73861 tcaactggca gatcccgcaa tccacttatc tggcctggct tgatctacgt ccgttgaata
    73921 ttgacgacaa cgcgttgcaa aaagcgctta tcgagcaaga aaagatcgcg atcatgccgg
    73981 gatataccta cggtgaagaa ggtcgtggtt ttgtccgact caatgccggc tgcccacgtt
    74041 cgaaactgga aaaaggtgtg gctggattaa ttaacgccat ccgcgctgtt ggttaacccc
    74101 aattgcgcaa cgtaaaaaat cgttgcgcaa tcgtggattt ttaccctgct ttgtttttat
    74161 aatggtgcgc acttttatat ccagaaaaag agtgcgacca tgattgatac caccctgcca
    74221 ttaactgata tccatcgcca ccttgatggc aacattcgtc cccagaccat tcttgaactt
    74281 ggccgccagt ataatatctc gcttcctgca caatccctgg aaacactgac tccccacgtt
    74341 caggtcattg ccaacgaacc cgatctggtg agctttctga ctaaacttga ctggggcgtt
    74401 aaagttctcg cctctcttga tgcctgccgc cgcgtggcat ttgaaaacat tgaagatgca
    74461 gcccgtaacg gcctgcacta tgtcgaactg cgtttttcac caggctacat ggcaatggca
    74521 catcagctgc ctgtagcggg tgttgtcgaa gcggtgatcg atggcgtacg tgaaggttgc
    74581 cgcacctttg gtgtgcaggc gaagcttatc ggtattatga gccggacctt cggcgaagcc
    74641 gcctgtcagc aagagctgga ggccttttta gcccaccgtg accagattac cgcacttgat
    74701 ttagccggtg atgaacttgg tttcccggga agtctgttcc tttctcattt caaccgcgcg
    74761 cgtgatgcgg gctggcatat taccgtccat gcaggcgaag ctgccggacc ggaaagcatc
    74821 tggcaggcga ttcgtgaact gggggcggag cgtattggac atggcgtaaa agccattgaa
    74881 gatcgggcgc tgatggattt tctcgccgag caacaaattg gtattgaatc ctgtctgacc
    74941 tccaatattc agaccagcac cgtggcggat ctggctgcac atccgctgaa aacgttcctt
    75001 gagcatggca ttcgtgccag cattaacact gacgatcccg gcgtgcaggg agtggatatc
    75061 attcacgaat ataccgttgc cgcgccagct gctgggttat cccgcgagca aatccgccag
    75121 gcacagatta atggtctgga aatggctttc ctcagcgcag aggaaaaacg cgcactgcga
    75181 gaaaaagtcg ccgcgaagta acaaaaatgg atggtgctaa ttgcaccatc cccttttcac
    75241 gcaaggcaca aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt
    75301 acctggattg cctgacttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga
    75361 atagccgcat agtaagccgg ataattcccc ggcactgtca acagcgtttc ttcgacacgt
    75421 tcctcacctt ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc
    75481 ggcagacgct cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa
    75541 ctgcctcggg atccatgcac gatataccgt gctgactcag cggctgccag catggtgccg
    75601 tgtaaaatca ctcgccgctg cggataggac aagatagcgt ggaaataatc ggtcgactgc
    75661 gctccgggcc gtaactgtgc caaatctacc gtcatgctaa ccggtaaacc aaatagcgta
    75721 atggcctgat caagaaggtg cggtgctaaa tcgtaccaga taccgctgcc tgggccgccc
    75781 tgttcacgcc aacgatcgcg tacctgcgga cggaagcggt caaaatgaga ctcaaagtaa
    75841 gcaacttccc ccagcacgcc ttccgcgagc aaacctttta gggtcagaaa atcgctatcc
    75901 cagcgacggt tatggaatac agacaacaca cgccccaggg tttttgccag cgcatccagc
    75961 tctcgtgctt gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc
    76021 tcaagcgccg ctttagctaa cggaaaatgg gtatcgttgg gtgtaggaat gacaatcagg
    76081 tctatgttgg gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag
    76141 tcggctttta cttttgtttc atcgctgctg gagattaccg ccagttccag cccgggcgtg
    76201 cccgcaatca ggggcgcatg gaaggttttg ctcgcataac catacccaat caccccaaca
    76261 cggatgttgt cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc
    76321 ctttaccctc gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa
    76381 gtcaaaaaaa tagcgatttt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa
    76441 aatcttgctg gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc
    76501 aacagagatg tgggctgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt
    76561 cgtttcagtc attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca
    76621 gagtactacg gtacaacaca agtaagctct gcacttgttg agcgacatgc tgcccgtccg
    76681 ggtgcatgtt ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcgatgttgt
    76741 tgatgattgt cagggatagt aaagcgcgtt actcttttca gagcagtttt tcatccctga
    76801 atatttgcca tcagcgcatt cttattattg taataacaca agcgttcact tttggtgaaa
    76861 ttttatcatc actaataata cctacccctt tggtatta
//
LOCUS       DACADA010000004        75202 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.004, whole genome shotgun sequence.
ACCESSION   DACADA010000004 DACADA010000000
VERSION     DACADA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.004"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1542
                     /locus_tag="ISB17_000247"
     CDS             <1..1542
                     /locus_tag="ISB17_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1978230.1"
                     /translation="IYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQF
                     SEMTDLVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERANKLLSLI
                     SYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAE
                     KYAQCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQ
                     GENAWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFL
                     VYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMG
                     EFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVI
                     ERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTA
                     GKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSG
                     KLVKE"
     gene            1550..2878
                     /locus_tag="ISB17_000248"
     CDS             1550..2878
                     /locus_tag="ISB17_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1978231.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2925..4250)
                     /gene="rimO"
                     /locus_tag="ISB17_000249"
     CDS             complement(2925..4250)
                     /gene="rimO"
                     /locus_tag="ISB17_000249"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAP1978232.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4463..4846
                     /gene="bssR"
                     /locus_tag="ISB17_000250"
     CDS             4463..4846
                     /gene="bssR"
                     /locus_tag="ISB17_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAP1978233.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4957..6072
                     /locus_tag="ISB17_000251"
     CDS             4957..6072
                     /locus_tag="ISB17_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAP1978234.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6069..6695)
                     /locus_tag="ISB17_000252"
     CDS             complement(6069..6695)
                     /locus_tag="ISB17_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP1978235.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDTDF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6942..8144
                     /gene="dacC"
                     /locus_tag="ISB17_000253"
     CDS             6942..8144
                     /gene="dacC"
                     /locus_tag="ISB17_000253"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP1978236.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8191..8949)
                     /gene="deoR"
                     /locus_tag="ISB17_000254"
     CDS             complement(8191..8949)
                     /gene="deoR"
                     /locus_tag="ISB17_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAP1978237.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHISKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9007..9603)
                     /gene="ybjG"
                     /locus_tag="ISB17_000255"
     CDS             complement(9007..9603)
                     /gene="ybjG"
                     /locus_tag="ISB17_000255"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP1978238.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9888..11120
                     /gene="mdfA"
                     /locus_tag="ISB17_000256"
     CDS             9888..11120
                     /gene="mdfA"
                     /locus_tag="ISB17_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAP1978239.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11161..11445)
                     /locus_tag="ISB17_000257"
     CDS             complement(11161..11445)
                     /locus_tag="ISB17_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978240.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11531..12346)
                     /gene="ybjI"
                     /locus_tag="ISB17_000258"
     CDS             complement(11531..12346)
                     /gene="ybjI"
                     /locus_tag="ISB17_000258"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAP1978241.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            complement(12346..13554)
                     /locus_tag="ISB17_000259"
     CDS             complement(12346..13554)
                     /locus_tag="ISB17_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1978242.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMVLSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATMHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13638..14174
                     /locus_tag="ISB17_000260"
     CDS             13638..14174
                     /locus_tag="ISB17_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1978243.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(14350..16035)
                     /locus_tag="ISB17_000261"
     CDS             complement(14350..16035)
                     /locus_tag="ISB17_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAP1978244.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16304..16681
                     /locus_tag="ISB17_000262"
     CDS             16304..16681
                     /locus_tag="ISB17_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978245.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16711..16968)
                     /gene="grxA"
                     /locus_tag="ISB17_000263"
     CDS             complement(16711..16968)
                     /gene="grxA"
                     /locus_tag="ISB17_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAP1978246.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17128..17415
                     /locus_tag="ISB17_000264"
     CDS             17128..17415
                     /locus_tag="ISB17_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAP1978247.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            17399..18121
                     /gene="nfsA"
                     /locus_tag="ISB17_000265"
     CDS             17399..18121
                     /gene="nfsA"
                     /locus_tag="ISB17_000265"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAP1978248.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18182..19084
                     /gene="rimK"
                     /locus_tag="ISB17_000266"
     CDS             18182..19084
                     /gene="rimK"
                     /locus_tag="ISB17_000266"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAP1978249.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19172..19648
                     /locus_tag="ISB17_000267"
     CDS             19172..19648
                     /locus_tag="ISB17_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAP1978250.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            19998..21110
                     /gene="potF"
                     /locus_tag="ISB17_000268"
     CDS             19998..21110
                     /gene="potF"
                     /locus_tag="ISB17_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAP1978251.1"
                     /translation="MIALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21205..22338
                     /gene="potG"
                     /locus_tag="ISB17_000269"
     CDS             21205..22338
                     /gene="potG"
                     /locus_tag="ISB17_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAP1978252.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22348..23292
                     /gene="potH"
                     /locus_tag="ISB17_000270"
     CDS             22348..23292
                     /gene="potH"
                     /locus_tag="ISB17_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAP1978253.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            23289..24134
                     /gene="potI"
                     /locus_tag="ISB17_000271"
     CDS             23289..24134
                     /gene="potI"
                     /locus_tag="ISB17_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAP1978254.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            24194..24682
                     /locus_tag="ISB17_000272"
     CDS             24194..24682
                     /locus_tag="ISB17_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAP1978255.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            24723..25850
                     /gene="rlmC"
                     /locus_tag="ISB17_000273"
     CDS             24723..25850
                     /gene="rlmC"
                     /locus_tag="ISB17_000273"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAP1978256.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(25879..26610)
                     /gene="artJ"
                     /locus_tag="ISB17_000274"
     CDS             complement(25879..26610)
                     /gene="artJ"
                     /locus_tag="ISB17_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1978257.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(26836..27504)
                     /gene="artM"
                     /locus_tag="ISB17_000275"
     CDS             complement(26836..27504)
                     /gene="artM"
                     /locus_tag="ISB17_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAP1978258.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(27504..28220)
                     /gene="artQ"
                     /locus_tag="ISB17_000276"
     CDS             complement(27504..28220)
                     /gene="artQ"
                     /locus_tag="ISB17_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAP1978259.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(28227..28958)
                     /gene="artJ"
                     /locus_tag="ISB17_000277"
     CDS             complement(28227..28958)
                     /gene="artJ"
                     /locus_tag="ISB17_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1978260.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(28976..29704)
                     /gene="artP"
                     /locus_tag="ISB17_000278"
     CDS             complement(28976..29704)
                     /gene="artP"
                     /locus_tag="ISB17_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAP1978261.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(29922..30437)
                     /locus_tag="ISB17_000279"
     CDS             complement(29922..30437)
                     /locus_tag="ISB17_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1978262.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            30563..30886
                     /locus_tag="ISB17_000280"
     CDS             30563..30886
                     /locus_tag="ISB17_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP1978263.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            30883..31713
                     /locus_tag="ISB17_000281"
     CDS             30883..31713
                     /locus_tag="ISB17_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP1978264.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(31710..32723)
                     /locus_tag="ISB17_000282"
     CDS             complement(31710..32723)
                     /locus_tag="ISB17_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1978265.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(32822..34252)
                     /locus_tag="ISB17_000283"
     CDS             complement(32822..34252)
                     /locus_tag="ISB17_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1978266.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(34263..35264)
                     /gene="ltaE"
                     /locus_tag="ISB17_000284"
     CDS             complement(34263..35264)
                     /gene="ltaE"
                     /locus_tag="ISB17_000284"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAP1978267.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(35301..37019)
                     /gene="poxB"
                     /locus_tag="ISB17_000285"
     CDS             complement(35301..37019)
                     /gene="poxB"
                     /locus_tag="ISB17_000285"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAP1978268.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGNGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(37152..38120)
                     /gene="hcr"
                     /locus_tag="ISB17_000286"
     CDS             complement(37152..38120)
                     /gene="hcr"
                     /locus_tag="ISB17_000286"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAP1978269.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            complement(38132..39784)
                     /gene="hcp"
                     /locus_tag="ISB17_000287"
                     /gene_synonym="priS"
     CDS             complement(38132..39784)
                     /gene="hcp"
                     /locus_tag="ISB17_000287"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAP1978270.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(39928..40827)
                     /gene="lysO"
                     /locus_tag="ISB17_000288"
     CDS             complement(39928..40827)
                     /gene="lysO"
                     /locus_tag="ISB17_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAP1978271.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(41242..41937)
                     /gene="aqpZ"
                     /locus_tag="ISB17_000289"
     CDS             complement(41242..41937)
                     /gene="aqpZ"
                     /locus_tag="ISB17_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAP1978272.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            42362..44020
                     /locus_tag="ISB17_000290"
     CDS             42362..44020
                     /locus_tag="ISB17_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAP1978273.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(44017..44973)
                     /locus_tag="ISB17_000291"
     CDS             complement(44017..44973)
                     /locus_tag="ISB17_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAP1978274.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            45124..46239
                     /gene="macA"
                     /locus_tag="ISB17_000292"
     CDS             45124..46239
                     /gene="macA"
                     /locus_tag="ISB17_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAP1978275.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            46236..48182
                     /gene="macB"
                     /locus_tag="ISB17_000293"
     CDS             46236..48182
                     /gene="macB"
                     /locus_tag="ISB17_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAP1978276.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(48255..48479)
                     /gene="cspD"
                     /locus_tag="ISB17_000294"
     CDS             complement(48255..48479)
                     /gene="cspD"
                     /locus_tag="ISB17_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAP1978277.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            48802..49122
                     /gene="clpS"
                     /locus_tag="ISB17_000295"
     CDS             48802..49122
                     /gene="clpS"
                     /locus_tag="ISB17_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAP1978278.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            49153..51429
                     /gene="clpA"
                     /locus_tag="ISB17_000296"
     CDS             49153..51429
                     /gene="clpA"
                     /locus_tag="ISB17_000296"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAP1978279.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(51773..51860)
                     /locus_tag="ISB17_000297"
     tRNA            complement(51773..51860)
                     /locus_tag="ISB17_000297"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51824..51826),aa:Ser,seq:gga)
     gene            complement(52114..52332)
                     /gene="infA"
                     /locus_tag="ISB17_000298"
     CDS             complement(52114..52332)
                     /gene="infA"
                     /locus_tag="ISB17_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP1978280.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(52617..53321)
                     /gene="aat"
                     /locus_tag="ISB17_000299"
     CDS             complement(52617..53321)
                     /gene="aat"
                     /locus_tag="ISB17_000299"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAP1978281.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(53363..55084)
                     /gene="cydC"
                     /locus_tag="ISB17_000300"
     CDS             complement(53363..55084)
                     /gene="cydC"
                     /locus_tag="ISB17_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAP1978282.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(55085..56851)
                     /gene="cydD"
                     /locus_tag="ISB17_000301"
     CDS             complement(55085..56851)
                     /gene="cydD"
                     /locus_tag="ISB17_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAP1978283.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(56974..57939)
                     /gene="trxB"
                     /locus_tag="ISB17_000302"
     CDS             complement(56974..57939)
                     /gene="trxB"
                     /locus_tag="ISB17_000302"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP1978284.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            58483..58977
                     /gene="lrp"
                     /locus_tag="ISB17_000303"
     CDS             58483..58977
                     /gene="lrp"
                     /locus_tag="ISB17_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAP1978285.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            59112..63218
                     /gene="ftsK"
                     /locus_tag="ISB17_000304"
     CDS             59112..63218
                     /gene="ftsK"
                     /locus_tag="ISB17_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP1978286.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            63377..63988
                     /gene="lolA"
                     /locus_tag="ISB17_000305"
     CDS             63377..63988
                     /gene="lolA"
                     /locus_tag="ISB17_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAP1978287.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            63999..65342
                     /locus_tag="ISB17_000306"
     CDS             63999..65342
                     /locus_tag="ISB17_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP1978288.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            65433..66725
                     /gene="serS"
                     /locus_tag="ISB17_000307"
     CDS             65433..66725
                     /gene="serS"
                     /locus_tag="ISB17_000307"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP1978289.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(67031..67171)
                     /locus_tag="ISB17_000308"
     CDS             complement(67031..67171)
                     /locus_tag="ISB17_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAP1978290.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(67363..67623)
                     /locus_tag="ISB17_000309"
     CDS             complement(67363..67623)
                     /locus_tag="ISB17_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAP1978291.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHDLPASHRGNNQMSLPLSQN"
     gene            complement(67664..68773)
                     /locus_tag="ISB17_000310"
     CDS             complement(67664..68773)
                     /locus_tag="ISB17_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP1978292.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            68931..70115
                     /locus_tag="ISB17_000311"
     CDS             68931..70115
                     /locus_tag="ISB17_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAP1978293.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGRTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            70115..70627
                     /locus_tag="ISB17_000312"
     CDS             70115..70627
                     /locus_tag="ISB17_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP1978294.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            70683..71057
                     /locus_tag="ISB17_000313"
     CDS             70683..71057
                     /locus_tag="ISB17_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1978295.1"
                     /translation="MKDIDTETRNNTVADDVTSGEDMAVERGVKLSRPIERGAEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            71066..71221
                     /locus_tag="ISB17_000314"
     CDS             71066..71221
                     /locus_tag="ISB17_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP1978296.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            71208..74015
                     /locus_tag="ISB17_000315"
     CDS             71208..74015
                     /locus_tag="ISB17_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1978297.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     QARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRLLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            74028..74516
                     /locus_tag="ISB17_000316"
     CDS             74028..74516
                     /locus_tag="ISB17_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1978298.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(74545..75144)
                     /locus_tag="ISB17_000317"
     CDS             complement(74545..75144)
                     /locus_tag="ISB17_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1978299.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
BASE COUNT        18429 a        18674 c        19982 g        18117 t
ORIGIN      
        1 atctattttt attatcctgg tggtaattta ttgaacgcat cagataaact tttttatctg
       61 ccgtttgctt tcattattat cgtattgctg gtggtttatt taatgaccac tcgagttttc
      121 cgtcgtcaat tttctgaaat gacagatttg gtcaatacgc tggcgttttt gcctgactct
      181 acggatcaaa tcgaggccct gaagattcgt gaaggcgatg cgaaagagat tatcagcatc
      241 aaaaattcga tcgcggaaat gaaagatgct gaaattgaac gggcaaataa attgctctcg
      301 ctgatctctt acgatcagga aagcggtttt attaaaaata tggcgattat tgagtccaac
      361 aataatcagt atctggctgt ggggatcatc aaactgtgtg gtctggaagc tgtggaagcg
      421 gtgtttggtg ttgatgaacg caataaaatc gtcaggaaat tgtgtcagcg aattgccgag
      481 aaatatgcgc aatgctgcga tatcgtgaca tttaatgccg atctctattt actcctgtgc
      541 cgggaaaatg tacagacgtt tacccgtaag atagcgacgg taaacgattt tgacagcagt
      601 tttggctacc gcaatctgcg catccataag tctgctattt gtgaaccttt gcagggggaa
      661 aacgcctgga gttacgcaga aaagctgaaa ctggcgattt ccagtatccg caaccatatg
      721 ttctcagagt ttattttctg tgatgatgcg aaactcaacg aaatagaaga gaatatctgg
      781 attgcgcgta atattcgcca tgcaatggaa attggcgaac tattcctggt ctatcaaccg
      841 attgttgata ttaacacccg caccattttg ggcgcggagg cgttgtgccg ttgggtgtct
      901 gcggagcggg ggattatttc accgctgaag ttcattacca ttgctgaaga tatcgggttt
      961 atcaatgagc tgggctatca aattattaaa accgcgatgg gtgaattcag acattttagt
     1021 cagcgtgcgt cgctgaagga tgatttctta ctgcatatta atgtttcgcc ctggcagtta
     1081 aacgaaccac actttcatga gcgttttact accatcatga aagaaaatgg cctgaaggcg
     1141 aacagccttt gtgttgagat caccgaaacc gtgatcgagc gaattaatga acatttctat
     1201 ctcaatattg aacaactgcg taaacaaggg gtacgggtat cgattgatga ctttggcacc
     1261 ggtttgtcaa acctgaaacg tttttatgaa attaatccag acagtataaa ggtggattcg
     1321 caatttaccg gcgatatttt cggtactgcg ggaaaaattg tgcgcattat tttcgatctg
     1381 gcacgctata accggatccc ggtgattgcg gaaggcgtag agagcgaaga cgttgcgcgt
     1441 gaattaatca aattaggatg tgttcaggct caggggtatc tgtaccagaa acccatgcca
     1501 ttctccgcct gggataaaag tggaaaatta gtaaaagagt agtttacgta tgtccagaat
     1561 caataaattc gtacttacag tcagtctgct gatttttatc atgatttcag cagttgcctg
     1621 cgggatctac actcaaatgg taaaggaacg ggtgtatggc ctgaaacagt ccgttattga
     1681 tactgctttt gcggtggcaa atattgctga atatcggcgg agtgtggcaa ttgatctgat
     1741 caacacgcta aatcctacgg aggaacagct gttggttggc ttacgcacag cttacgccga
     1801 ctcggtttca ccctcttatt tgtacgatgt cggtccttat ctgatttcca gtgacgaatg
     1861 tattcaggta aaggagtttg agaaaaatta ttgtgcagat attatgcagg ttgttaagta
     1921 tcgacatgtc aaaaatacag ggtttatctc ttttgatggt aaaaccttcg tctattacct
     1981 ctatccggta actcacaatc gtagtctgat atttttgctt ggtctggaac gtttttcttt
     2041 actgtcaaaa tcgctggcga tggatagcga gaacctgatg ttctccctgt tcaagaacgg
     2101 taaatcggtg accggtgatg aatataacgc taaaaacgcc atcttcaccg tttcggaagc
     2161 aatggagcac ttctcctatt tgccgaccgg attgtacgta tttgcttata aaaaagacgt
     2221 ttattttcag gtatgtacat tgattatttt ctttgccgca ttggtggcag tgatatcggg
     2281 tgccagttgc ctctatctgg tacgcagagt gattaatcgt ggtattgtgg agaaagaagc
     2341 cattattaat aaccattttg aacgcgtact ggatggcggg cttttctttt cggctgccga
     2401 tgtcaaaaaa ctctacagta tgtataactc ggcattcctg gacgacctga ccaaagcgat
     2461 gggcagaaaa tcctttgacg aagatttaaa agcgctgccg gaaaaaggcg gttatttgtg
     2521 cctgtttgac gtcgataaat tcaaaaacat taacgacacc ttcggtcatt tgctgggcga
     2581 tgaagtgttg atgaaagtgg tgaaaatcct taaatcacag atcccggtag ataaaggtaa
     2641 agtctaccgc ttcggaggtg acgaatttgc ggtgatttat actggcggca cgctggaaga
     2701 gttgctatcg attctaaaag aaatcgttca tttccaggtg ggaagcatta atttaagcac
     2761 cagtatcggt gtagcgcatt caaatgaatg tactaccgtc gaacgattga aaatgctggc
     2821 ggatgagcgg ctgtataaga gtaagaaaaa cggcagggcg cagattagct ggcagtaatc
     2881 attattcgca ggccggacaa atgattttgc ccggcctgaa ttaattaaac ccggctaccc
     2941 cacaaatcgt actcatcggc gtgctcgact ttcactcgca ggatatcacc cggcttaacg
     3001 ttggtttcac cattgagata aaccgcgccg tcgatttccg gtgcatctgc catgctgcga
     3061 ccaatcgcgc cttcttcgtc cacttcgtcg ataatcacca gaatttcacg acccactttc
     3121 tcttgcaggc gctcggcgga aatctgctgc tgcagctgca tgaaacggtt ccagcgttct
     3181 tctttcactt cttccggaac ctggtcaggc agggcattgg cgtctgcacc ttcaaccggg
     3241 ctgtatttaa agcagccaac gcgatccaga cgtgcttctt tcaggaagtc gagtagcatc
     3301 tggaaatctt cttccgtctc gccagggaag ccaacaataa aggttgagcg tagggtcagt
     3361 tccgggcaga tttcgcgcca ctgtttgatg cgcgccagtt ggcgatctac agaacccgga
     3421 cgcttcatca gtttgagaat gcgcgggctg gcgtgctgca acggaatgtc cagatacggc
     3481 aggattttgc cctctgccat cagtgggatg acgtcgtcca catgcggata agggtaaacg
     3541 tagtgcagac gtgtccagat ccccagtttc gataactgtt cgcacaggct gaccatgctg
     3601 gtttttaccg gctcgccgtt gtggaagcca gtacgatgtt taacatcgac gccatacgcg
     3661 gaagtatcct gcgagatcac cagaatttct ttaacgcccg catcaaccag acgtttcgct
     3721 tcacttaaca cttcgccaat cggacggctc accagatcgc cgcgcataga cggaataatg
     3781 cagaaggtgc agcggtgatt acagccttca gaaattttca gataagcata atgacgcggc
     3841 gtcagtttca caccttgttc tggcaccagg ctcaggaacg ggttgtgttt cggttttggc
     3901 acgtagtgat gaacgtgctc cagaacctgc tcatagctat gaggcccggt gatttccagc
     3961 actttcgggt ggacttcgcg gatctgatct tcttttgccc ccagacaacc ggtcacaatt
     4021 accttgccgt tttcattcaa cgcttcacca atggcttcca gtgattcttg taccgcgctg
     4081 tcaataaagc cgcaggtgtt gacgatcacc atgtccgcat cgtcatagct cggtaccacg
     4141 tcataacctt cagtgcgtag ttcggtgaga atacgctctg aatcgacaag gtttttcggg
     4201 cagccaaggg aaacaaagcc gattttcggc tggggagtta ctttgctcat agcttaaaaa
     4261 atattcagtt acaggaaagg tcagggcagg gattctacag agttctggat aaaatttgta
     4321 tcgcaatctc attcgctggc ggagttgaag gaaatgtaaa ttttgttaat tcggcgtgaa
     4381 gaattgatcc tggacagcat tttgctcaaa aaacagccat actatttaat tgcaacaagg
     4441 ctggaaagag gaggatcgaa gtatgttcgt tgacagacag cgaatcgacc tgctgaaccg
     4501 gttgatcgac gcacgcgttg acctcgccgc atacgtgcaa ctgaggaagg caaaaggata
     4561 catgtccgtc agcgaaagca atcatctacg agataacttt tttaaactga atcgcgaact
     4621 gcacgataaa tcgctgcggt tgaatcttca tctggatcag gaagagtgga gtgctcttca
     4681 tcatgctgaa gaagcattag cgacagccgc agtatgtttg atgagtgggc accatgattg
     4741 cccgactgtt attaccgtca acgccgataa gcttgaaaat tgtctgatga gcttaacgct
     4801 gagtatccag agcctgcaga agcacgccat gcttgagaag gcctgaaaac taagggggag
     4861 aaagcgtctc ccccttcatg tttaagtttt tgtaaaaatg aatttgttct ctcctccact
     4921 gactacgctt taagccagag tcaatccgga ggcgttatgc atcgacaatc ctttttcctt
     4981 gtgcccctta tttgtctttc ttccgctctc tgggcggctc ctgcaacggt aaatgtcgaa
     5041 gtactgcaag acaaactcga ccatccctgg gcgctggcct ttttacccga taatcacggt
     5101 atgttaatca ctctgcgcgg cggcgagttg cgtcactggc aagcaggaaa aggattatct
     5161 gcgccgcttt ccggagtgcc ggacgtttgg gcgcacgggc agggcggcct gctggacgtg
     5221 gttttagcgc ccgattttgc tcagtcgcgc cgcatctggt taagttattc cgaagttggc
     5281 gatgatggca aagccggaac tgctgtgggt tatggtcgct taagtgatga tctctcaaaa
     5341 gtgaccgact tccgcaccgt ctttcgtcag atgccaaaac tgtctaccgg caaccatttt
     5401 ggcgggcgga tggtatttga cggtaaaggt tatcttttta ttgctctggg cgaaaacaat
     5461 cagcgcccga cggcgcagga tctggataaa ttacagggca aactggtgcg tctgaccgac
     5521 ctgggagaaa tcccggatga taatcctttt ataaaggaat ccggtgtgcg cgccgagatc
     5581 tggtcttatg gcattcgtaa tccgcaagga atggcgatga atccgtggag taatgcacta
     5641 tggctgaatg aacatggccc gcgcggtggt gatgaaatta atattccgca aaaaggcaaa
     5701 aactacggct ggccgctggc aacctgggga atcaactatt cgggctttaa gataccggaa
     5761 gcgaaagggg agatcgtcgc cgggaccgag caacctgttt tttactggaa agattcgccc
     5821 gccgtgagcg gcatggcttt ctataacagc gacaaattcc cccagtggca gcaaaaatta
     5881 tttattggcg cgctgaaaga taaagatgtc attgtgatga gcgtcaacgg cgacaaagtg
     5941 acagaagatg gccgtatttt aacggacaga gggcaacgaa ttcgtgatgt tcgcactgga
     6001 cccgacggtt atttatacgt tctcaccgac gagtccagtg gggaattact taaagttagc
     6061 ccacgcaatt agctaacggg aatcatcacc actttgcgca ctgcggggcg ttcagtgagt
     6121 tgctgatacc agcgttgcag atttggacgc ggtgtccagg tcaggccaac gttaaacaaa
     6181 ttgtagatga acggtgcgat agcgatatcg cccacaccaa actcgtcgcc tgagaaccat
     6241 tttacttttg ccaattccgc atcgagcagg gcaaacaggg cgtcgcactc tttgcaactg
     6301 gcatcaatgg cggcctgatc gcgctcttcc ggtggtgttc tgactaatcc catcaggatc
     6361 ccgcgatgag cattactgag cgtctggttt gcccagtcca tccatttttc tgcttccgca
     6421 cgacgtgccg gtgagtcgat ccacaggcgt ttttgcccgt actgtgccgc cagatagcgg
     6481 acaatggcgt ttgattccca aagaatgaga tcactttcgt cgtcacgcaa caacggcacc
     6541 agcccgttag ggttcatcgc cagaaaatca gtatcgtgat ttatcccaaa ctcacggccc
     6601 gcgagaattt gctcataagg tagttccagt tcttcgagcg tcagcaatac tttttttacg
     6661 ttggttgaat tattccgacc ccacagcgta atcatactca ccccttttcc gacaggcagc
     6721 cacaggctaa acaggactcc gatggtgaag taaagttaac ttttaagcaa cagctggcaa
     6781 aaaaatgaca ccagaagcag cgaagcggca ggatattgca taaactttaa aaactttacc
     6841 aacttacggt ttctttaagt ttgtgtgtgc gttattaatc accgaactta tcatacggcg
     6901 atataacgta ttttttttga atggatactc gggtggcatt tatgacgcaa tactcctctc
     6961 tccttcgtgg tcttgcagcg ggttctgcat ttttattcct ttttgcccca acggcattcg
     7021 cggcggaaca aaccgttgaa gcgccgagcg tggatgcccg tgcatggatt ttaatggatt
     7081 acgccagcgg taaagtgctg gcagaaggca atgcggatga gaaactggat cccgcgagcc
     7141 tgactaaaat tatgaccagc tatgtggttg ggcaggcgct taaggccgat aaaattaaac
     7201 tcaccgatat ggtgacggtc ggtaaagatg cctgggcgac gggaaatccg gcactgcgtg
     7261 gttcatcggt aatgttcctc aaaccgggcg atcaggtttc ggtggcagac ttgaacaaag
     7321 gtgtgattat ccagtccggt aatgacgcct gtattgcgct ggctgattac gttgccggga
     7381 gccaggagtc atttattggt ttgatgaatg gttatgccaa aaaactgggt ctgaccaaca
     7441 ctaccttcca gacggtgcac ggcctggatg cgccggggca gttcagtacc gcgcgcgata
     7501 tggcattgct gggtaaagca ttgatccacg atgtgccgga agagtacgcc attcataaag
     7561 agaaagagtt caccttcaac aaaattcgtc agcctaaccg taatcgtctg ctgtggagca
     7621 gcaatctgaa tgttgatggc atgaagacag gaaccacggc aggcgcggga tataatctgg
     7681 ttgcttcggc tacccagggc gatatgcgtt taatctccgt agtgctgggg gcgaaaactg
     7741 accgtatccg ttttaatgag tctgagaaat tattgacctg gggtttccgc ttctttgaaa
     7801 ccgtgacgcc aattaaacct gatgccacat ttgtgactca gcgcgtctgg tttggtgata
     7861 agagcgaagt gaatctcggg gcaggcgaag cgggctcagt gaccataccg cgtgggcagc
     7921 tgaaaaacct gaaggcgagt tatacgttaa cggaaccgca gcttaccgca ccgctgaaaa
     7981 aaggtcaggt tgtcggcacc attgatttcc agcttaacgg taaatccatt gagcagcgtc
     8041 cgctgatcgt gatggaaaat gtggaagagg gcggattctt tggtcggatg tgggatttcg
     8101 tgatgatgaa attccatcag tggttcggca gctggttctc ttaatcttct gataaccgga
     8161 tggcgcgaaa cgtcatccgg ttatacgtca ttaatacatc aacttaatac gctgcgtctg
     8221 cgcgtacttc acatactcat cttccgggca acaatcgctc acaacaatat caaagcgttt
     8281 caggtcaccc atgcgcgccg gacgcacctt gccaaattta ctgtggtcga caaccagcac
     8341 atgcttttgc gccatcgaca tggcccagtg ttttaccggc aactcttcaa gattaaaaca
     8401 ggtagcgcct ttactgatat gcacgcccgc agcggaataa aaagcgatat ccgggcaaaa
     8461 attattcagt gtttgctgaa aatcgatggg tttgaaaatg gcgttgctgg cgtgaaattc
     8521 accaccgcaa agaaacgcgc ggcaatgggg tttctctttc agcgccagaa aagtatttag
     8581 cgaataacaa acggcggtaa aagggatttc attatcaatt gcttcaataa tccacggcgt
     8641 ggtggtgcca cagtcaaaaa agagggtctg atcgggttct accagcgtcg cagccagttt
     8701 tgccgcccgg cgtttttctt ccaccaggcg ggatttttga tcgcttaaca ggtaatggct
     8761 ggcgctgcgt ggttccagaa caatatagcc gccgagcaaa acgacgggcg cactgtggtt
     8821 gttcagatcg cgacgaatcg tcatctccga aaccccaagc agggcggcgg cgtctttaag
     8881 atgtaactta tcgctgcgtt ttaattcttg cagcagctgc ccgatacgct cttcgcgacg
     8941 tgtttccata atccctctga atagttattg aagcgagccg ctcaatacta cactttttag
     9001 cagagatcag tcacgcaccc agcctttgcg gatcggtaat gcaaaacaga agcgatacca
     9061 cgattgcaga cgttgataga gtttatgccc catcgcttgc cagataatct gggcgctaag
     9121 gcagccaatc atacctgcca gcaatccacc gagcatatcc agcggccagt ggacgccaag
     9181 ataaacgcgc gaccaggcaa tgacgacggc cagcaccatt aaaagtgagc cggaccacag
     9241 gcgatgccag cataaaaatg ccagtgcaaa ggtgaaaatc accgtaccgt gatcgctcgg
     9301 gaatgagtca tccgccgcat gatgcaggaa gttatagccg atattttcga caaagggtcg
     9361 gtcgtgcgga aaaagatgtc ccatcgtcca ggacacaaac aggctgaccg ccagcgcgat
     9421 agcgattttt atcaccagtt gccgttgtgc tgtaagcccc cacaaccaaa gtactgcggc
     9481 cagcaacggc accacggtaa tcaaatcttt agcaataaaa atcgccaacg agatcatcca
     9541 cggcgcagag tctggcgttg cgttaataag agagaataga gagagattca aattttccag
     9601 cataacttcc cgacgcaaag tgattaaaag ggagccaata caggcaagtc gttgagaata
     9661 aagtgcaggt taaactgggt aaagcggcat cgtcttattt ccctcaagcg gcctgtttac
     9721 ggtaggtgat tgtaacgggc aaaggttaaa taaaacttaa agaaagcgta gctatactcg
     9781 caataatgta agaatgtgct taaccgtggt ttcagctaca aaattcgctt tctcgttagc
     9841 tgcgctttta ttaaactttg cgcgattatt attggcgaag aaattgcatg caaaataaat
     9901 tagcttccgg tgccaggctt ggacgtcagg cgttactttt ccctctctgt ctggtgcttt
     9961 acgaattttc aacctatatc ggcaacgata tgattcaacc cggtatgttg gccgtggtgg
    10021 aacaatatca ggcgggcatt gattgggttc ctacttcgat gactgcgtat ctggcgggcg
    10081 ggatgttttt acaatggctc ctggggccgt tgtcggatcg tattggccgc cgtccggtga
    10141 tgctggcggg agtggtgtgg tttatcgtca cctgtctggc aatattactg gcacaaaaca
    10201 ttgaacaatt caccctgttg cgcttcttgc agggtataag cctctgtttc attggcgctg
    10261 tgggatacgc cgcaattcag gaatccttcg aagaggcggt ttgtatcaag atcaccgcgc
    10321 taatggcgaa cgtggcgctg attgctccac tacttggtcc gctggtgggc gcggcgtgga
    10381 tccatgtgct gccctgggaa gggatgtttg tcttgtttgc cgcattggca gcgatctcct
    10441 ttttcggtct gcaacgagct atgcctgaaa ccgccacgcg tataggcgag aaactgtcgc
    10501 tgaaagaact cggtcgtgac tataagctgg tgctgaagaa cggtcgcttt gtggcggggg
    10561 cgctggcgct gggattcgtt agcctgccgt tgctggcgtg gatcgcccag tcgccgatta
    10621 tcattattac cggtgagcag ttgagcagct atgaatatgg cctgctgcaa gtgcctattt
    10681 ttggggcgtt aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac
    10741 gttcgctgat tattatgggc ggctggccga ttatgattgg tctgttggtc gctgctgcgg
    10801 caacggttat ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt
    10861 tcggtattgg tctggcgaat gcgggactgg tgcgattaac cctgtttgcc agcgatatga
    10921 gtaaaggtac ggtttctgcg gcgatgggga tgctgcaaat gctgatcttt accgtcggta
    10981 ttgaaatcag caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc
    11041 ttgtcaacgg cattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg
    11101 gaaattcgca cgaagggtaa aaaaatgcct gattgctttg tgcgatcagg cattgtcgaa
    11161 ttaatggtga tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag
    11221 atggcgaaca taaggatgcg ggcggtaagc cggagctggc gcaggagcca catagacggt
    11281 ttgcggcaca gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac
    11341 cactttgtat ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa
    11401 gcccattaaa agcgcgccca acagtagaca atttttcata atcatttcgc ctgaaataat
    11461 gcagcgggtt aatttccgcg aattatgcag ccttcattca ggtattgtgt tgccgggcgt
    11521 gctgcctttt ttattgctta aatggcgctt cgtgtttaag cactttatcg atcacatcca
    11581 gtacgccttc acggttattg gagcctgccc ggtattttgc cgctgcgacg accgcgctgc
    11641 cggcattttc cattgcaaag ctaaaccctg cctgacgcag catctcaata tcgttaccgc
    11701 catcgccaaa gaccaccact tcgctgtcgt ctattcccca taatttctgc agttggcgaa
    11761 ggccattggc tttatgtaca ccggggataa tcagatcgat gctgccgttg ccggtatgga
    11821 ccggtaccat gatatcgccg atggcctcat gtaatgcttt ttgcacttgt gggatcagtt
    11881 cgtcagagag attaaggcca aatttaaaga agatatcttc taagttgtca aagttatcga
    11941 cgtattccag acggtgataa tacatttccg ccaccgtttt catggcatcg tcatattttt
    12001 tgagggtata ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca
    12061 aatgttccac gacagtagta aacgcatcct tcgacagctc gccattaaaa acatctttgc
    12121 cttcgctcac tacccagccg ccgttttcag ccacaaaggc aatttcatta gcaatttcag
    12181 gaaagaaaga gatcaactga taatattgat tcccgctggc gaccacaaag cgaatgcctt
    12241 gtgctttcat ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc
    12301 ttaagaaagt accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat
    12361 cgccgtctgc gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat
    12421 aaccagcgcc agtacaacca tcattgcact acgtaatcca taatgttcgc cgagatagcc
    12481 cagcagcggc ggcccgacga ggaaagccag ataaccggtc gtagctacca cgctgacgcg
    12541 ggttggtgca tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga
    12601 ggcacccagt ccccaaagta caacagacac cccagcgacc caggcgctat cgacaaaaat
    12661 aatcagccca atacccaacg cccccattag tgcactggcc cgaaccacgg caacgcgact
    12721 gtaacggtca atgaaccaac cgccggtaaa gcgtccaacg gtcatcccca gagtaaaacc
    12781 ggcataaatc agcgagccgg aagtagggct aaaaccgtga ccatcaacca ttaataaggg
    12841 taaccagtcg ttggcagaac cttcggcaaa ggccatcgcc agcactacaa caccaatcaa
    12901 cagcaactgg atatcgcgat aaaaaggaac gcctttttcg ccatgctggg tgccatcggc
    12961 agcatttttg cccgtaccat cagggattgc ctgaatggcg atataaatag gcgcgatgcc
    13021 taccagcgcc gccaataaaa tgtgcatcgt tgccggaacg ccaaaggccg tcagtgccat
    13081 cccgacacca gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca tcggtaaaac
    13141 cgtcttattc atttctctct caacggcggc accttcaacg tttatcgcca cttccgcaga
    13201 accaaaacta gccccaaaga cgccgagacc aacggcaaag agcaagggcg atgtcagcca
    13261 gagtgccaga cttaatacca tcatcccgat caatgcgcag gacatcgtga caaggatgac
    13321 attacgcgtc ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc
    13381 gatcgacaga ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg
    13441 gatagcaggc gtacgggttg cccaggacgc cattaacagg cctggcaaaa agaagaacat
    13501 aaacagcgcc caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagacca
    13561 cgtcaaaata agaagaggga agacaacatt agcaagactg tgtacatttg tacacaattg
    13621 cagagagagg aaatgacatg cgtcgcgcta acgatccgca acgacgagaa aaaattatcc
    13681 aggccacgct ggaggcggtg aaactttacg gaatacatgc tgttacacac cgcaaaattg
    13741 ctacccttgc cggggtgccg ttgggatcga tgacctacta tttttcagga attgatgagt
    13801 tgttgctgga ggcgttcagc cgttttactg agatcatgtc ccggcaatat caggcatttt
    13861 ttagcgatgt tagtgatgct ccaggcgcat gccaggctat caccgatatg atctacagct
    13921 cacaggtcgc aacgccggat aatatggagc tgatgtacca gctctacgcg ctggctagcc
    13981 gaaaaccgct cttaaaaacg gtaatgcaaa actggatgca gcgcagtcaa caaacgctcg
    14041 aacaatggtt tgaacccgga accgcccgcg cgcttgatgc gtttattgag ggaatgacgc
    14101 tgcattttgt caccgaccgt aagccgctat cacgcgagga gattttgagg atggttgaga
    14161 ggattgctgg gtagtagata aatttcaggc aacaaaaaac ccatcaacct tgaaccgaaa
    14221 tggcggggtt gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta
    14281 agactgatgc cctgcggaaa agttctgcgg ttgtgcaaaa aaaatttcat tttcagggca
    14341 acttcagttt tatcctaatc ctggccatac catgacgatg attgtccctg ccagcgtcag
    14401 caggacgttg gcgattgcat aggtgcccgc atagcccagc gccgggatgt tactgcgagc
    14461 tgtatcactg atgatctcca ttgccggcgc gcaggtgcgt gcgcccatca ttgcgccgaa
    14521 caacagtgcg cggttcattc gcaatacata agcaccgaac aagaaacaga taaccactgg
    14581 cacaagactg acaatcaatc cggcaatcaa catctgaccg ccaatcgcgc ccaggccgtt
    14641 attaataccg ctaccggcgc tcagaccaac gcctgccata aacaccatta agccgaactc
    14701 tttcaccatg cttaatgcac cctgcggaat gtaaccgaag gtcgggtggt tagcgcgcat
    14761 aaatcccagc ataattccgg cgaataacaa cccggcagcg tttcccatgc cgaaactgaa
    14821 tgtgctgaac tggaaggtga tcatcccgat catcagccca ataacaaaga aggcgcagaa
    14881 tgccagcaag tcagtgacct ggctatgaat cgagataaag ccgatgcgat cggcgatggt
    14941 ttttacgcga cgggcatcgc cgctgacttg taaaacgtca cctttgttaa gcacgacgtt
    15001 gtcatctatc ggcatctcaa tctggctacg aatgacgcgg ttaaggaagc aaccgtgatc
    15061 ggtcaacttc agttgtgcga gacgtttgcc aacggcgtta tggtttttaa cgaccacttc
    15121 ttcagtgacg atacgcatgt cgagaaggtc acgatcgaaa acttctttgc cgttacggaa
    15181 gctgggatcg agtcgggcgt gggcgtcggg atagcctacc aacgctattt catcgcccat
    15241 ttgtagcacc gcatcaccgt ctggatttgc cagaatcccg ttacgtcgaa tacgttcaat
    15301 gtagcagcca gtttgtcgat aaatacccag ttcacgcaga tttttgccgt cggtccaggc
    15361 caccagctcc gggccgacgc ggtaggcgcg gatcaccggt aaataaacct tacggttggc
    15421 atcagtgtcc aggccacgtt cgcgggcgat ttgctgggcg ctggtctgta agtcctgatg
    15481 ctgcaatttc ggcaagtaac gcgcaccaac aatcaaactc accagaccga ttaaataggt
    15541 taaggcatac ccgaggctca gattatccag tgccagtgag agctgcctgc tttccatgcc
    15601 ggaatgacgc agtgtatcgc cagcaccgac cagaaccggt gtcgacgtca tagagccagc
    15661 taacataccg gccgtcaggc caatatccca gccaaacagc ttacctaacc ctaaggcgat
    15721 caccagcgca ctgccaacca tcaccagtgc taacattagg taatttttcc catcgcgaaa
    15781 aaaaatggaa aaaaagttcg gtccggcttc gaccccgacg cagaaaataa acagcataaa
    15841 gccaagatta agcgcgtcgg tgttaatgct gaaatgttgt tggcctaata acagcgatac
    15901 gactaaaacg ccaatggaat tacccagttg gatcgaacca agtcgtaatt tcccgagaca
    15961 tagcccaagc gcgaggacca caaataataa cagaatgtaa ttcccattta acaattcggc
    16021 gacgtttata ttcacggagg ctaacttctt gtttactagt aagctgttga aagaaatggt
    16081 aatttacgat aatgttttta ccagaattca gggcgcagat tcattcagcg cacctaaacg
    16141 atagtaaagt aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga
    16201 attgcgttgg cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac
    16261 tggcgaaaaa tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg
    16321 ttgggcgggg gcaatctgct gttttgtcct cttcattgtg gtgtgtcttt ttctggcgac
    16381 gcacatgaaa ggcgcttttc gggctgccgg gcatcctgaa atcggtttgc tatttttcat
    16441 tcttcctggt gcagttgcca gcttcttttc acatcgtaga gaagtcctga aacctctgtt
    16501 tggcgcaatg ctggcggcac cctgctcgat gctcattatg cggctgtttt tttcaccgac
    16561 gcgctcattc tggcaagagc tggcatggtt attaagtgcg gtgttctggt gtgcgctggg
    16621 ggcattgtgt ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcaata
    16681 aagccctcaa tgcgagggct tgtcagacga tcaggcgtcc agattctctt tcacccatgc
    16741 agcaaaatcg gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc
    16801 tacgggttta cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc
    16861 tacatactga tactggaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc
    16921 acgcacacag taagggcaac ccggacgacc aaaaataacg gtttgcatta tttctctcct
    16981 catagattta tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct
    17041 gttgcattaa ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact
    17101 gaaggctcac tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaagcgtg
    17161 ttgatactgg aatttatcgg aatgatgttg ctggcggtgg cgctgctgtc ggtaagcgac
    17221 tccctgtcgc tacctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc
    17281 ggtgttttgc tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt
    17341 gccccacagc tgatgaacca tccaccgcaa tattcacgtt cagaaagaga aaaagataat
    17401 gacgccaacc attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc
    17461 catttccgaa gcgcagcgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag
    17521 ttttttgcag tgcagtagca ttatccgcat taccgacaaa gcgttacgtg aagaactggt
    17581 gacgctgacc ggcgggcaaa aacacgtagc gcaagcggca gagttttggg tgttctgtgc
    17641 cgactttaac cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact
    17701 gttgctcggt gtcgttgata cggcaatgat ggcgcagaat gcattaaccg cagcggaatc
    17761 gctgggattg ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgga
    17821 actgctgaaa ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc
    17881 ggataatccg gatcttaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta
    17941 tcaaccgctg gataaagacg cactggcgca gtatgacgag caactggcgg aatattacct
    18001 cacccgtggc agcaataatc gccgggatac ctggagcgat catatccgcc gaacaatcat
    18061 taaagaaagc cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta
    18121 aaactgccac gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag
    18181 ggtgaaaatt gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga
    18241 agccgctata cagcgcggtc acctggttga aattcttgat ccgctttctt gctatatgaa
    18301 cataaatcct gcggcgtctt ctattcacta caaaggccgc aagttacccc attttgacgc
    18361 agtgatcccg cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt
    18421 cgagatgctg gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa
    18481 attgcgttcc atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc
    18541 gcattcgccg gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt
    18601 caagctggtt gaaggcacgc agggaattgg cgtcgtgctg gcggagacgc gtcaggcggc
    18661 ggaaagcgtg attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat
    18721 caaagaggcg caagggtgcg atatccgctg tctggttgtt ggcgatgaag tagtcgctgc
    18781 gattgaacgg cgggcgaaag agggcgattt tcgttccaat ttgcatcgtg gcggcgcggc
    18841 aagcgtcgcc agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat
    18901 ggcgctggac gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga
    18961 agtgaatgcg tcgccagggc tggaaggaat agaaaaaacc accggtatcg acatcgcggg
    19021 taaaatgatc cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg
    19081 ttagtcgcaa tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg
    19141 gtctttttat cggaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga
    19201 tacgttgcgt caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca
    19261 ggctctgcat ctgccccata tgcagaattt cgacggtgtc tttgatgcta aaatcgatct
    19321 gatcgataac acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc
    19381 gctggcggcg gatttatcag ccatcaatgc cagttcgctg accgtgaaag catttcttga
    19441 tatgcaggat gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg
    19501 cgtaacctat gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt
    19561 cattcttgaa gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaataa
    19621 cgttaccgaa aactatttcc tccactgata acttctttcg agcacgcagt cgctgccgca
    19681 gtgactgcgt gccgcaaaat tatccgctga ttttaacctt ttattaaaga ttaattcact
    19741 tctcttgtgc cgatttggct ttatcacata gagcaaatac gcataaaaat ttgttaaata
    19801 ccgtttttta atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttt
    19861 tatatagtga gcagtgcagg ccgggagaga gttctctttt cttacaccgc gccgataaaa
    19921 atatgcacgt ttattgcata tctttcagtg tgacaacttt tgttcgtttg ttaacgaatt
    19981 ttcagaagga aagagatatg atcgccttaa ataaaaaatg gctatcgggt ctggttgcgg
    20041 gtgctctgat ggccgtttct gtcagcacgc tcgcggctga acaaaaaaca ctccacattt
    20101 ataactggtc tgattatatc gccccggaca cggtggccaa ctttgaaaaa gaaaccggta
    20161 ttaaagtcgt ctatgatgtt ttcgattcta acgaagtact ggaaggcaaa ttaatggcgg
    20221 ggagcaccgg ctttgatctg gtggtgccgt cagcaagctt tcttgagcgc cagctgacag
    20281 cgggagtttt ccagccgctg gataaaagca aattgccgga gtggaagaat ctcgatccgg
    20341 agctgctgaa gttggtcgcc aaacacgatc ccgacaataa atttgctatg ccctatatgt
    20401 gggcgacgac cgggattggc tataacgtcg ataaagttaa agcggtgctg ggcgaaaacg
    20461 cgcccgtcga tagctgggac ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct
    20521 gcggtgtctc tttcctggat gcgccagaag aagtgtttgc taccgtgctg aattatctcg
    20581 gcaaagatcc taacagcact aaagcggatg attacaccgg accggctacc gatctgctgt
    20641 taaagctgcg cccgaacatt cgttatttcc attcatccca atacatcaat gatctggcga
    20701 atggtgacat ctgtgtcgct attggctggg cgggcgatgt ctggcaggcg tcaaaccgcg
    20761 cgaaggaagc gaagaatggc gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg
    20821 cgttctttga tgtattcgcc atgcctgcgg atgccaaaaa caaagacgaa gcctatcagt
    20881 tcctgaatta cctgctgcgt cctgatgtgg tggcacatat ctctgaccat gtgttctatg
    20941 ctaacgccaa taaagcagcc acgccgctgg tgagtgcgga agtccgtgat aacccgggta
    21001 tttatccgcc tgcggatgtt cgtgcgaagc tgttcactct gaaagtgcag gacccgaaaa
    21061 tcgaccgtgt gcgcacccgc gcctggacca aagtgaagag cggaaaataa tccgcagccg
    21121 tagatgccgg aggggcgcac cacacccgcc ggcaattcgc accatcatgg tgcgcttgca
    21181 cacattcaat gccggagagc agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc
    21241 cgtaaggcgc tgacgccgct attagaaatc cgtaacctga ccaaatccta cgatggtcaa
    21301 catgcggtgg atgatgtcag cctgaccatc tacaaaggtg aaatcttcgc gctgctgggc
    21361 gcatccggct gtggcaagtc cacgctgctg cgtatgctgg caggttttga acaaccttcg
    21421 gccgggcaga ttatgctcga tggcgtcgat ttgtcacagg tgccgcctta cctgcgcccc
    21481 atcaatatga tgtttcagtc ttacgcgctg tttccgcata tgaccgtgga acagaacatc
    21541 gcttttggcc tgaaacagga caaactaccg aaagcggaaa ttgccagccg ggtcaatgag
    21601 atgcttgggc tggtgcatat gcaggagttc gccaaacgca aaccgcatca gctttctggt
    21661 ggtcagcgtc agcgtgtggc cctggccaga agccttgcca agcgcccgaa actattactg
    21721 ctcgatgagc cgatgggcgc gctggataaa aagctgcgtg accggatgca gcttgaagtg
    21781 gtggatattc tggagcgcgt cggtgtgacc tgcgtaatgg ttactcacga tcaggaagag
    21841 gcgatgacta tggcggggcg catcgccatt atgaatcgtg ggaaatttgt ccagattggc
    21901 gagccggaag agatctacga gcatccgacc actcgctaca gtgctgaatt tatcggttcg
    21961 gttaatgtct ttgaaggtgt actcaaagag cgtcaggaag atggtctggt gctttattcg
    22021 ccggggctgg tgcatccact gaaagtcgat gcggatgctt cagtggtcga taacgtgccg
    22081 gtgcatgtgg cgcttcgccc ggaaaaaatc atgctttgcg aggaaccgcc cgccaatggt
    22141 tgtaacttcg cggtggggga ggtgatacac attgcctatc tcggcgatct ttcggtgtat
    22201 cacgttcgtc tgaaaagtgg gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat
    22261 cgtaaagggt taccgacctg gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt
    22321 gtggtgctga cggtttaagg agcaaagatg agtacacttg aacctgctgc ccagccaaaa
    22381 ccctttaagc tgtggttgtc gcagctgcaa atgaaacatg ggcgcaaact ggttattgcg
    22441 ttgccgtata tctggttgat cttgctgttt ctgctgccat ttctgatcgt ctttaaaata
    22501 agcctggcgg agatggcgcg cgctattcca ccttataccg aattgatgga gtgggctgac
    22561 gggcaacttt ctatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    22621 tattttgatg cttatctcca gtcgttacag gtggcggcga tttcgactat ttgctgttta
    22681 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatc
    22741 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgattcgtgt ctacgcctgg
    22801 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct ggggattatc
    22861 gatcaaccgc tgaccattct gcataccaac tttgcggttt atattggcat tgtttacgcc
    22921 tacgtgccgt ttatggtgct gccgatttac accgcgttga ttcgtattga ttattcgctg
    22981 gtagaagcgg cactggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    23041 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    23101 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgctg
    23161 tggcaggagt tctttaacaa ccgcgactgg ccggtggcct cagcggtagc gatcgtcatg
    23221 ttgctgctgc taattatgcc gataatgtgg ttccacaaac atcagcaaaa aagcgaggga
    23281 gaacacggat gaataattta ccggtggttc gttcgccctg gcggattgtg attttgctgt
    23341 tgggcttcac ctttctctac gcgccaatgc tgatgcttgt tatctattcg tttaacagct
    23401 cgaagctagt gacggtgtgg gccggctggt caacgcgctg gtatggcgaa ttattgcgcg
    23461 atgacgcgat gatgagtgcg gttgctttaa gtctgacaat tgcggcctgt gcggcgacgg
    23521 cggcggcgat cctcgggact attgcggcgg tcgtgctggt gcgctttggc aggtttcgcg
    23581 gatcaaatgg ttttgccttc atgatcaccg caccgctggt aatgccagat gtcatcacgg
    23641 gtttgtcgct gttgttgtta ttcgtcgcgc ttgctcatgc tattggctgg cctgcggacc
    23701 gcggtatgct caccatctgg ctggcgcatg tcactttctg tacggcttat gtggcagtcg
    23761 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    23821 gtgcgacgcc gctgaaagtg ttttttgtca ttacgctacc gatgatcatg cccgcgatca
    23881 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    23941 tttccgggcc gggagccacc acattaccga tgctggtctt ttccagcgtg cggatggggg
    24001 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    24061 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccagcgtg
    24121 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    24181 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    24241 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    24301 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    24361 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttgagcagtc tggtgctggt
    24421 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    24481 gtatctgttg acacaaatca cagccgcaag ttacttgtgg gcggcttcgc tggggtatgg
    24541 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    24601 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    24661 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    24721 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    24781 cgattccaga gcaactctcc gctaaaactg ccgatcttaa aaatctgctc gccgattttc
    24841 cggttgagga atggtgtgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    24901 aaatggtggt gagtggtagc gttgaaaaac cactgctcgg tatgctgcat cgagatggta
    24961 caccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    25021 cgctaaaacc gtttatcgcc cgtgcggggt taacgcccta caacgtggcg cgtaaacgtg
    25081 gtgaactgaa atacattctg ctgactgaaa gccagagcga tggtggcatg atgctgcgtt
    25141 ttgtactgcg ttctgatacc aaactggcgc aattgcgtaa ggcgctgccg tggttacagg
    25201 aacaattacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    25261 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcagag cgttttaatg
    25321 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    25381 agctgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    25441 tgttctgcgg tgtgggtggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    25501 ggatcgaaat tgcaccagag gccattgcct gtgcgaagca gtcagccgct gaactgggct
    25561 taacgcgttt gcaatttcag gcgctggact ctacgcagtt tgccaccgct cagggggaag
    25621 tgccggagct ggtgctggtt aacccgccgc gccgcggcat cggtaaaccg ctgtgtgatt
    25681 atctctcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaactatgg
    25741 cgaaagatat ccgcgaactg cctggttatc gcatcgaacg ggtacagctt tttgatatgt
    25801 tcccgcacac cgcgcactat gaagtgctga cgctactggt gaagcaataa aaaagccgca
    25861 ggtgcggctt tctgaatctt actgtgggaa ccactggtca ttgattttct gatatgtacc
    25921 gtcagcttta attgctgcca gcgcgttatt cagtttttcc agcagagctt tgttatccgg
    25981 acgtacagcg atacccaggc cggtgccgaa gtattgcgga tcggtcactt tttcagtagc
    26041 aacgcccagt tgcggattgg ttttcagcca ttcgtttacc actgcggtgt caccaaatac
    26101 gccatcaata cggccatttt tcagatcgat aaaggcattc tgataactgt cataggagac
    26161 agttttcact tccgggtgct gatcctgaat atatttctgg tgcgtggtgc cgttttccat
    26221 cccaatacgt ttgcctttca gatcggcaaa cgttttgtag gtatcttttt tggcaatcac
    26281 gacggctgag ttttcgtagt agggcgtggt aaacgatacc tgtttgctac gctccggcgt
    26341 gatatccatt ccggagatta cggcgtcata ttttctgaat ttcagtgacg ggatcaggct
    26401 gtcgaaagcg tgattagtaa aagtacattc tgcctgcatt tgtttgcaca aggcttttgc
    26461 caggtcgata tcaaagccga caatctcatt attagcacct atagattcaa agggtggata
    26521 ggtggctgaa acgccaaaat tgattttctc tgcggcagaa gcaccgaaag taaaagaagc
    26581 aagtaaagcg gcaagaacta actttttcat gatggaactc ccgtctgtca atcttatgat
    26641 ttttggccgt gtctgcggca tgggataaca atgccattaa gtgaatttat atgcaataaa
    26701 tatgagtaaa taatttatat gaaataaaaa aagacggaca gcttagtggg ttgtccgtct
    26761 tcataataag aatttatgca ctatgtaggc cggataaggc gtccacgccg catccggcat
    26821 cagcatgcag ctacattaat tccgccgttc gaatgccagt gctttacgct cgatcagacg
    26881 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccccgccg cgccaaatac
    26941 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatgagcgt
    27001 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    27061 gagcgagcgt ttaaacgcgt acggcagcag gatcgccagc gtatcttttt tgctcattcc
    27121 cagggcgcta caggactgcc attgaccttc cgggatcgcg cgaatcgcac cgtaaaacag
    27181 ctgcgtggtg tacgccgcac tgttcagcga caacgcaatc agcgcacata accacggttc
    27241 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc gggaactggc ccggcccgta
    27301 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tatagccccg
    27361 taccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    27421 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    27481 cagttcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    27541 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    27601 acaatgtacc aggtaaacgg ttcctgggta cgggtagcga tgcttttggt ttgtagcatt
    27661 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    27721 ttaccgagac ccggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    27781 gccgatttcg acagccccag cgcctgaccg gattcccact gccccaccgg caccgctttt
    27841 aatgcaccac gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    27901 ccacaaagga acgggctaac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    27961 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gcagctgcga ggagccaaaa
    28021 tagataaaca gcaccaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    28081 cctgcccacg cgacaggacg ccatttcgca gactcccata ccgcaaagaa catcgccagc
    28141 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    28201 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    28261 taagtgccat ctttcttcac tttttccagc gcagtgttga atttctgctg cagttcagtg
    28321 ttgccctggc gtactgcgat gccgaggcca gtaccgaagt aatctttatc ggtcacttta
    28381 tcgcccactg ccgccagttt cgggttatct ttcagccatt cagttaccac tgcggtgtca
    28441 ccaaatacgc tgtcgatacg accgttttgc agatccagtt ttgcgttctg gtaactgtca
    28501 tatggcacgg tagtgatttc cggatgctta tccataatga acttctggtg tgtcgtcccg
    28561 ttctgaacgc caactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    28621 cccacaaaca gggcagagtt gtcatagtac ggggtggtaa acagcacctg cttttcacgc
    28681 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    28741 atcaggctgt caaacgcctg gttagagaaa gtgcaggttg catcaatctc tttacacaat
    28801 gcctgcgcca ggtcgacatc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    28861 ggaggatagg aggcttcggt ggcaaaacga atggtttcgg ctgctgtggc ggaaagacta
    28921 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    28981 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    29041 gttctacgat atgaccgttt tccatataca ccactcggct ggcggtttta cgcgccactt
    29101 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    29161 tgacgatttg tgccgtaatt tcggggtcca gtgcggcggt cggttcatcg aacagcagta
    29221 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    29281 gatgcagcgg gtaacgatcg ctataaggtt taagacgcag acgttccagc agtttttctg
    29341 cacgggccag cgcctgatct ttactcagcc ccagtacacg gcagggcgct tcaatcaggt
    29401 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    29461 gacgcaaatc gcgaatcgct ttgtcagagg gggttttggt gaaatcgaaa tggttgcctg
    29521 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    29581 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgcggg caatccagcg
    29641 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    29701 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    29761 cagaataatt atgcaatatt tctgctttaa aagttaaaag caaagcgcat tattcaataa
    29821 acatagcaca aaataacggg ggcggtggtc ggcgagcata aatgtcggca ttcctcacga
    29881 aatgccggac aatttacggg gtttattggt tgatcaagac gttagcgatt ctcgatggat
    29941 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    30001 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgc
    30061 ttgagatcca cgcgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    30121 gctacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    30181 tcacgatgac tattatcgcg gctggcatcg ctaagtaggg ttgccagttt gtcactcaaa
    30241 taggggcgca gagcggtaat gtcattgctg cgatgttgaa tgcggtagtc atagaactgt
    30301 tgagccacac tatcaggccc cccctggaca caggaaccgg tgcgggggcc gttatcttta
    30361 taagctggcg tgacggtggt acaggcactg agtagcagtg cgcaagggat aagcatggtc
    30421 aatttgctat agcgcatgat gatttcctta taagcgaaca ccttgaaagc gttctacgat
    30481 aataatgata tcctttcaat aatagcgtat cagtccgata atgctttgaa gatcgaaggc
    30541 ttagcaaaca aggagatcga tcatgcaatt ttctacaacc ccaactctgg aaggccagac
    30601 catcgttgaa tattgcggtg tggtgactgg cgaagcgatt ttaggcgcga atattttccg
    30661 tgatttcttt gccggtatcc gcgatatcgt tggcggacgc tccggtgcct acgaaaaaga
    30721 actgcgtaaa gcgcgggaga ttgcctttga ggagctcggc tcccaggcgc gagcgctggg
    30781 ggctgatgcc gtcgtcggta ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat
    30841 gctgatggtt agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag agtcttctgg
    30901 ctggtcgctg ccgctctgtt actggcaggg tgcacaggcg aaaaaggcat tgtcgagaaa
    30961 gagggatatc agcttgatac ccgacgccag gcgcaggcgg cgtatccgcg cattaaagtg
    31021 ctggtgatcc actacaccgc agatgatttt gatagctcgc tggcgacact gaccgataag
    31081 caggtcagct cgcattatct ggtccctgcg gtgccaccgc gatacaacgg taaaccgcgc
    31141 atctggcaac tggtgccgga acaagaactg gcctggcatg cggggattag cgcctggcgc
    31201 ggggcaacgc gtcttaacga cacctctatt ggcattgagc tggaaaaccg tggctggcaa
    31261 aaatcggcgg gagtgaaata ttttgccccg tttgaaccgg cacagattca ggcgcttatt
    31321 ccgctggcga aagatattat tgcccgttat cacatcaagc cggaaaacgt agtagcacat
    31381 gcggatattg cgccacagcg caaagacgat ccggggccat tatttccctg gcagcaactg
    31441 gcacagcagg ggattggtgc ctggccggat gcgcagcggg tgaactttta ccttgccggg
    31501 cgcgcgccgc acacaccggt agagaccgcg tcattgctgg agcttttggc gcgttatggt
    31561 tatgacgtaa agcctgatat gactccgcgc gagcagcggc gtgtgattat ggcgttccag
    31621 atgcatttcc gcccgacgtt atataacggc gaagcggatg cagaaactca ggcgattgcc
    31681 gaagcgttgc tggagaaata cgggcaggat tagcgcggca gttttccgtg gtcgcgcagc
    31741 caggcggcag ttttctcgat accttcatcc agggtgagga ctggctgata acctaactct
    31801 tcctgcgcac gcgtaatatc cagcgtaaag tcaaaattca acttggagac gccgtagtgg
    31861 gtcagcggcg gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata
    31921 tccagcatcg ggtaaggaac cgaacgaata cgacagtcaa tattcaactc gtcgatcagc
    31981 ttctgcacga tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta cacacgcccg
    32041 gaaggtagct tatcgcacgc ttcctggctt gccagccaca tggcgtgcac ggcattttca
    32101 tagtaggtca tatcgaccag cgcactgccg ccatgcggta acagaatact gccgtagtga
    32161 tgcatcatat gcgccagacg agggataaag actttatcgt gcggtccgaa cagactttgt
    32221 gggcgcagaa tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct
    32281 tcgctggctg ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct
    32341 ttaatatcgc gatggtggtg ataatcgaag tacagggagg gagaagagat atgaataaag
    32401 ttacgcacac cccaggcgac agcccattca cccaggcggc gagtggcgcg aacgttagcc
    32461 agatcgaaag cctgttgtgt cccccagggc gaggtaaagc tggagcagtg ccacagcgta
    32521 tcaatgcccg cgagcatcac tttagcttgt gatgagacca actcggtcag atccgccgga
    32581 acaaactctg cgcccatttt ttccagcaat ttacccattg cctcgttgcg accggtcgct
    32641 cgcacgctga tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg
    32701 gcgccggtaa ccagtacctt catatcaatc cactgttgtt gagaaaatga cgtgcgcatt
    32761 cttccgtgat ttcccccttg atgcaatggg aaacatgaaa gaataacgca ggttttgtcg
    32821 attaatctgt gctttgttct gccagtctgg cgattcgttt tgccattccg cgaaaaataa
    32881 acagatgcgc ggggatcatc aataaccagt aaaacagccc cggcataccg tgcggatgcc
    32941 agaaagcacg gacatcgatt gtacgatagt cgcctttatc ttccagggta aaacagagtc
    33001 gtcccagccc cggcgctttc atgccaaata acaacgtaag ttgtttttcc ggttcaacga
    33061 caatgacttt ccagctatcc acagcatcgc cggtctgtaa atattcgcgc tccgggcggc
    33121 cttttgccag cttatgaccg atcgcaaggt ccatcaacgc ccgtgtctgc cacaaaatat
    33181 tgccaaagaa ataacgctct ttaccgccga tttgattgac cacccgccat agcgcctgaa
    33241 ggctggcaga cgttttaacg gtaaaccccg cctgtttggc aaaataaccg tattctggtc
    33301 gccagcgagc aaaagcctgc gcgtcatatc cccagtcgct ggagttgacc agtttttctt
    33361 cctctttcaa cgtgctacgt accgcgtcat cgaaagcgat cagcggttgt ggaatgagtg
    33421 cacgtaacgc ggtgtcatcc gccagcagat cgtgtttcag cccctgaatc aacgctctgg
    33481 cggtggtggg cggtactgaa gtaatcacat taagaaacca caccgaaatc cagcgggtgg
    33541 ggagggggat ggggagcaac cagcggcgct taccgctcac cgccataaaa tgttcaaact
    33601 gttgctgata actgagcact tctggcccag cggcttcgaa gatgcgatgt tcgcaagccg
    33661 gatgatctaa cagcgccaca agatagtgca gcaggttttc cagcgcgatg ggcgtagtgc
    33721 gtgaacgtac ccagcgtggt ggcgttaata ctggcaggtt gtaaaccata tcgcgcatga
    33781 cttcgaatgc cgctgaaccc gcgccaacga taattccggc acgtagttcg gtcaccggga
    33841 cacccgcttc acgtaaaata tccgccgtgg cctgacgagc acgcagatga tcagattgtt
    33901 catgaggcgg tgcctgcaat gaactgagaa agatgacttg ctttaccggt acttcgcgca
    33961 gcgcatcgcg gacgttgaga gccacctggc gctcctgagc gataaaatca ccaccttcgc
    34021 ccatgctgtg caccagaaaa tagacagtat caatattctg caacaggacc ggaaggttat
    34081 ccggccagtt gagatcgact ttatggcaac tgacattagc cagttgcagt tttgcaagtc
    34141 tgtcgacgtg acgtgccgcc gccaggatct gatgcccttg ctggctgagt gtgtgcacca
    34201 gatgttgacc aatgtagcca ctggcaccga gaactaaaat gcgttgcggc acgtctctct
    34261 ccttaacgcg ccaggaacgc acgccagtgg gcggcaactt ccgccagttg ttcacgcgag
    34321 acgtcaagat gggtcaccag gcggacaatc ggcgaggcgt taatcagcac gtttctcgct
    34381 ttcatgtatt cgcctaacgc ggcagcattt tcttccccga cgcgaacaaa cagcatattg
    34441 gtgtcctgac gcatcacatc cgcgcctgct tcacgcaact gctccgccat ccaggcggcg
    34501 ttatcgtggt cttcctgcaa acgtgcgacg ttatttttca gcgcatacat cccggcggca
    34561 gccagaatgc cggactgacg catcccgcca cctgtcattt tccgccagcg aatggcacgt
    34621 ttaatgtaat cacgattacc gacgagtaat gaaccgactg gcgtcccaag acctttcgac
    34681 aggcaaatgg tgaacgagtc acaatattgc gtgatctctt tcagttcgca gccgtaagcc
    34741 accacggcat taaaaatgcg cgcaccgtca acgtgcagcg ccagattgcg ctcgcgggta
    34801 aattcccatg cttctttcag gtattcacgc ggcagcactt tgccgttgtg ggtgttttcc
    34861 agactgagta atttggtgcg ggcgaaatgg atatcgtcgg gtttgatttt catcgccact
    34921 ttatccagcg gtagcgtacc gtcggcagct gcgtcgatgg gttgcggctg aatactgccc
    34981 agtactgctg cgccaccggc ttcaaacaga tagttatgcg cggcctgacc gacgatatac
    35041 tcttcgccgc gttcgcagtg actgagcaga gcgaccaggt tggcctgagt gccggtaggc
    35101 agaaaaatgg cggcttcttt accggaaagc tctgctgcat agtcctgcag agcattaacg
    35161 gtagggtcgt ctccataaac gtcgtcccca accggggcgg ccatcatcgc ttcgagcatg
    35221 gcgcggctcg gtcgggtaac ggtatcactg cgtaaatcaa tcatggcatg tccttattat
    35281 gacgggaaat gccacccttt ttaccttagc cagttcgttt tcgccagttc gatcacttca
    35341 tcaccgcgcc cgctgatgat tgcgcgcagc atatacaggc tgaaaccttt ggcctgttcg
    35401 agtttgatct gcggtggaat ggctaattct tctttggcga ccaccacatc caccaacacc
    35461 ggaccgtcga tggagaaggc gcgttgcagg gcttcatcga tttcagacgc tttttctacg
    35521 cggatacccg taatgccgca ggcttcggca attcgggcaa agttggtgtc gtgtagttcg
    35581 gtgccgtcag tcaaatagcc accagctttc atctccatcg ccacaaagcc cagcacgctg
    35641 ttattaaaga cgataatttt cactggcagc ttcatttgca ctaccgagag gaaatcgccc
    35701 atcaacatgc taaaaccgcc atcgccgcac atggcgacca cctggcgttc tggctcggtt
    35761 gcctgcgcgc ccagcgcctg cggcatggcg ttagccatcg aaccatggtt aaacgaacct
    35821 aacaggcgac gcttgccgtt catttttaga taacgtgccg cccacaccgt tggcgtacca
    35881 acgtcacagg tgaaaatagc gtcatcggcg gcaaaatgac taatttgctg cgccagatat
    35941 tgcgggtgaa tggctttctc gctcggttta gctaaatcgt ccagcccttt gcgggcgtcg
    36001 cggtaatctt ccagcgcctt atccagaaac ttgcgttcgg ttttttcttc caccagtggc
    36061 agcaatgcgc gcagggttga tttgatatcg ccgaccagcg ccatatctac cttgctatgc
    36121 gcgccgatgc tggctgggtt gatatcaatc tgaataattt tggcatcggt cgggtagaac
    36181 acacggtagg gaaactgagt accgagcagc actaacgtat cggcgttcat catggtatgg
    36241 aaacctgacg aaaagccgat taatcccgtc attccaacat catatggatt atcgtattcg
    36301 acatgttctt taccgcgcag ggcatggact ataggcgctt taattttccc ggcaaactca
    36361 actaactctt tatgcgcccc cgcacagccg ttgccacaca tcagggcgat attgctggaa
    36421 taacgcagca gttgcgccag tttgcgtaac tcttcttctt ccggtgttac taccggctgt
    36481 ggcgcatgat accagtgggt agttgcccct tctggcgcag gttttaacgc cacatcgccc
    36541 ggtaacacaa caacggaaac gccacggtta agcaccgctt tgcgcatagc aattgccagc
    36601 acttgtggga tctgctctgg gctggaaacc agctcgcaat agtgactaca ttcgcggaat
    36661 agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga gggaatatga
    36721 gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc gttgattaag
    36781 tgcaggttgc cgggaccgca cgatccggca cagaccgcca gttctccgct aagttgtgct
    36841 tcagcgccag cggcaaatgc cgccacttct tcatgccggg tggacatcca ctcgatggtg
    36901 cccatgcgat taagactgtc actaagaccg ttcagagagt cgcccgtgac tccccagatg
    36961 cgtttcacgc ctgccgattc gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg
    37021 gttctccatc tcctgaatgt gataacggta acaagtttag ttcatctgac ggagggggaa
    37081 gggatgggag aaaaaggagg cactaacggt taaatagccc gatgaaagga atatcatcgg
    37141 gcatcaggcg attatgcgaa aaccaaatcc ccctgcggat ggcatgagca ggccagtacg
    37201 taaccttcag cgatttcggc gtcggtcagc gtcattgtgc tgctcaccgt atattcaccg
    37261 gaaatcactt tcgtcttaca gcagccgcaa acacccgcac ggcaggcggc gacaaccgga
    37321 acgttattgc tttccagcgc ctccagtagc gtggtgccaa ccggagcgta aaattctcgt
    37381 gccggttgca gtttggtgaa tttcagaccg ctggtcgccg cttccgctac tggggtgaag
    37441 aatttctctt taaagaaacg cgtcacgccg agcgttttca cttcctgctc tacccaatcc
    37501 atatatggag cagggccaca ggtcatcacg gtacgtgagg ctaaatcagg gacacctgcc
    37561 agcagttcgc gagtgagacg accagcgata aagccttcgg taacgttatt ttccgccacc
    37621 agcgttaccg gatagttacg ccactcatcg gcgaaaatca catcctgcgg cgtacgcacg
    37681 ttgtagatca cctgcacatc ggcctgtgga cggttcttcg ccagccagcg acgcatcgac
    37741 ataatcggcg tgacgccgca gcctgccgcc agcaacagga atttatcttc tgctttatcg
    37801 tcgcaggtaa attcccccat cgcgtccgaa agccagagat aatcaccgcg ttttacatcg
    37861 cgcgtcagcc accgggagcc gacaccgtca tcaatccgcc gcacagtcag ggtgatgtat
    37921 tcactcacgc ctggcgtgga ggaaatggtg taagcacgca gcgtttctgc tgagtttcgc
    37981 acgctgacca gcgcatattg cccggcgcga tacgggtagt aatcgtggca aatcagggaa
    38041 atcgtccaca catccggcgt ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat
    38101 tgattcgttg gcatcgtcat cgacaaactc cttacgcgct caacagttgc ttcatgtctt
    38161 cttcaacagt ggtgatagaa cgcaggccga atttctcgtt cagcaccgcc agcaggtctg
    38221 gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt tttcacaccc agagaaagca
    38281 gcgtcagcag aatgacaatc gctttctgtt caaaccagga gagcaccagc gacagcggca
    38341 gatcgttgac tccgcagccc agtttctctg ccagcgttac cgccagaata attgctgagt
    38401 aagcatcgtt acactgacca gcatctacca gacgcggcag accttcgata tcgccaaact
    38461 caagtttatt aaagcgatat ttaccgcaag cgagggtcag gatcaggcag tcatccggca
    38521 cgctggtggc gaaatcggtg aagtagtgac gctcgccgcg tgcgccgtca cagccgccaa
    38581 gcaggaagat atgacgcagt ttttcacggc tcaccagatc aatcagcgta tcggctgcgc
    38641 caagcagtgt ctggcgacca aagcccacgg tgatcaggtg cggaatttcg ctgtacggga
    38701 agcccgccat ctgttgcgcc tgggcaatga ccgcagagaa atcttcgcca tccagatggc
    38761 gcacacccgg ccagccaacg atgctgcggg tccagatacg atcgtcataa gcgcctacgg
    38821 ttgggtcgat gatgcagttc gaggtcatca cgatggggcc tgggaaacga gcgaactcca
    38881 cttgctgatt ctgccagccg ctgccgtagt taccgaccag atgcttgaat ttacgcagct
    38941 ccgggtaacc atgcgcaggc agcatttcgc cgtgggtgta gacattaacg cccgtgcctt
    39001 cggtctgttc cagcaggttg tagagatctt tgagatcgtg accggaaatc aggatgcatt
    39061 tacctgccgt cgctttgaca ttgacctggg ttggggtcgg gtgaccgtat ttaccggttt
    39121 cgcctgcgtc cagaatgctc atcactttga agttcatctg gccgatttcc attgaacact
    39181 caagaagcgc gttcatatcg gcaggccagg tccccagcca cgccatgatt ttatggtact
    39241 gggcataaat atcgttgtcg tattgaccga gaacatgcgc gtgttccata taggccgccg
    39301 cacctttcag gccatacagg cacagcagac gcaggccgag aatgttttcg ccaatcgccg
    39361 ctttatcttt gttaggggta aattctgctg cctgacgttg cagctcgccg agatcgtcgc
    39421 tcaccagttg caggtcagcc atcgggttat cgacgcgggc gttggcatct acagccaggc
    39481 attgtgcttt cagcgcctcg cgcagggcaa tcgcttcacg agcgtagccg ataatacgcg
    39541 gagaatcgaa gttaacgttg gtcagggttg agaaaaaggc acgtggcgca aagctgtcta
    39601 catcgtggtt gatgatgccg tattcacgtg ctttcactgc ccaggccgaa agcccttgca
    39661 gcgtcgcgat gagtaaatcc tgaaggtcag aggtttccgc cgttttacca cacatcccct
    39721 gcgcgtatga gcagccgttt cctgccggag tacggatagt ttgttcacat tgcacacaaa
    39781 acatgatcac accttttaaa gttatattta atatacatgt ttaaggttaa gacgcttaac
    39841 gcggggataa aagagatttt taatgcaact ttaagggaga ttgatttagc gcaattttgg
    39901 cggcagagat ctaccgccag agaggtatta tgcagagaaa aaggcgatga gaatcggcac
    39961 taacagacta agaataaaac cgtgaacaat cgccgccggg accatatcca gcccgcctgt
    40021 acgttgaaga acgggcaggg tgaaatccat cgacgtcgca ccgcataagc ccagtgcagt
    40081 agagcggcta cggcgaatca gcccagggat taacataata gcaatcagct cacgggccag
    40141 atcattaaaa aacgccgcgc tcccgattac cggaccaaaa gattcggtca ataaaatacc
    40201 ggaaagagaa taccagccga aaccggaggc cattgccagc gcggtattga tgggaagatc
    40261 aagaataaag gcgttaatta aaccaccaat taatgaactg gcaaccacca ccacggcgac
    40321 aatcattccc cggcgattaa ggacaatctg ctttaaggtc atgccattat tgcgcaactg
    40381 aataccgacg aggaaaagca gcagaattaa ggtgtattca ctggcttcgg tcgcgtgttg
    40441 taagaaagcc agtccactca gaccaatggc aaaaccaatc actactacgc cgcacagttt
    40501 tagcgactcc agcgccatcg caatacgcga cgggagtttt tcttgctgat ggtggttgct
    40561 ccatggcagg ccgcgctcca gccacatcag ggcggcaata ttacacagta aaataacggt
    40621 agtactgacg gcagaataat gcagaattgc caacaagtta ctggcgaggt tatcgagaaa
    40681 cgccagactg atacccataa aaaagagaat aaggtaaacc atccagctta agagctgatt
    40741 aataactctt aatgcagctt tttggcgaag cggaatgagg taacccacaa tcaggggaac
    40801 cagaatgatt aacagcccag aaaacatgaa aacccagtcc ttgcaaagaa gattcgaaat
    40861 gcgcgatgac acactactga aagcggaagg acgagtaaag ttgcaattaa aaggaaatgt
    40921 tatgcataag gatcggtagg gtattcattt tcatttaaag atattcttgc gctttaatta
    40981 caaaccgcac cggtgttggt ggcgtcaaaa tcgccgaccc ggtagccgta agggataagt
    41041 cgagggcacc gcgcagcggc gattttgttc gccagagccc gggggtgcag ggggcggcgg
    41101 cgattggccg ccccctgcac gctccttgcg ccagtggcaa tatgtggctt agctcatgaa
    41161 aggagcgcaa caagatgatg aatcaacata taacaacatc ttaaaaaaag gcctgacatt
    41221 gcgccaggcc ttctgcgtta attaattacg cttttccagt agagtccggt aaatcagacc
    41281 accgataata ccgccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca
    41341 gccgccctgg aagatagcaa ccgcagtact gcgcgccggg ttaacggaag tgttagtcac
    41401 cggaatactg attaagtgaa tcagggttaa ggccagacca atggcaattg gcgcaaaacc
    41461 agcaggagcg aatttgtcag ttgcgccgtg gatcaccagc aggaaacctg cactcaatac
    41521 cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc
    41581 gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata
    41641 cagcagcgcc gccgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc
    41701 ttttgccggg aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc
    41761 accagaaata tgaccaacag caaaggccat tgtcagaacg gtcagaccga acgccaacgc
    41821 cacgccggca aaaccaatgc ctaattccgg gaagcctgcg gccagtacgg cactaccaca
    41881 gccgccaaaa acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc
    41941 cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa
    42001 aagtgacgac ggaaataatg cgcggctatt ttaaaaacga aggcaaatca ttcgccagat
    42061 aaataaatcc agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc
    42121 tgattgacag tgtagtgcaa gcgaaagatt taatccttta ggcgtaataa aaaataattt
    42181 atcatgctaa ttatttgatt ttgttgtttt tgcaggctta tcagcaagag ggagtataac
    42241 gcgattattc gctcattttt cagacatttg ccatgcttaa atgtgatgcc atcacgtatt
    42301 agcaggcctt tcccgttata ctgccagcgt aaagaataag tcacatattt ctggagggga
    42361 tatgattctt gagcgcgttg aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat
    42421 gctggaacaa aacaacgtcc tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga
    42481 cgccttaacc ctgctgctat cgccagaatc agatctctac cattttgagc gcgatgattt
    42541 ctggttcccg ccgggagata tcaacgggcg agaacatcat ctgcatatta ttttgacctt
    42601 ccgcgaatcg ctgccaggcc gacatcgggt tcgccgctat cggccgctgg aagcgtgctg
    42661 gacgccatgc accgatggtt atcaccgtat tttttatcgt ctggaagggg agagtgcgga
    42721 agacggcagc gtgatgacac tgcgcagttt tctcgataaa gacggacatc cgattgatgt
    42781 tgaggatatt aacgatcagg ctcgtcatct ggtgcgttta atgccggtgc tgcgcttgcg
    42841 tgatgcccgt tttatgcgcc gtattcgtaa cggcacggtg ccaaatgtcc ccaatgtgga
    42901 agtcaccgcg cgccagctcg atttcctcgc ccgtgagttg tcctcacatc cgcaaaatct
    42961 ctctgatgga cagattcgtc agggactttc cgcaatggtg caactgcttg agcattattt
    43021 ttctgagcag ggagctggac aggcgcgata tcgtttaatg cggcggcgag ccagcaatga
    43081 gcaacgaagc tggcgctatc tggatatcat caaccggatg attgaccgac ctggagggcg
    43141 ctcgtatcgg gttattttgc tcggcctgtt tgctactttg ttgcaggcaa aaggcacatt
    43201 gcgactggat aaagacgccc gtccattgtt gctgatcgaa gatccagaaa cccgtttgca
    43261 ccccattatg ctttcagttg cctggcatct gttgaacctt ctgccattgc agcgcatcgc
    43321 tactaccaac tcgggagagt tgctttcgtt aacgccggta gagcatgttt gccgactggt
    43381 acgtgagtcc tctcgcgttg ccgcctggcg tctgggaccg agtggcttga gtaccgaaga
    43441 tagccgacgc atctcctttc acattcgttt taaccgtccg tcatcgctgt ttgcccgttg
    43501 ttggctgctg gtggaagggg aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg
    43561 cggacatcat tttgatgccg aagggatcaa ggtcattgag tttgcccagt ccgggctaaa
    43621 gccactggtt aaatttgccc ggcgaatggg gattgaatgg catgtactgg tcgatggcga
    43681 tgaagcaggg aagaaatatg ccgctacggt acgcagcctg ttgaataatg atcgggaagc
    43741 cgaacgagaa catttaacgg cgttaccggc gctggatatg gaacatttta tgtatcgcca
    43801 gggattttcc gatgtgttcc accgcgtggc gcaaatcccg gaaaatatac cgatgaatct
    43861 gcgcaaaatt atctcgaaag cgatccatcg ctcttccaaa cccgatcttg ccattgaagt
    43921 ggcaatggag gccggacgtc gtggtgtgga ctccgtaccg acgctgctga aaaaaatgtt
    43981 ctcacgcgtg ctgtggctgg cgcgcggtcg ggccgactaa ctacaaaaca tcgtggccat
    44041 ttgtggctga atagcgtcga gcatctcata gcgccgacgg tattcagccc gttttttact
    44101 ggcgatttcg gcaatctctt ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc
    44161 atcacatacg ccgccaaccg attcccagaa agcgttgtaa tcggcatgga tcttgccttc
    44221 tttatcgcga taacgcaggc tgcggtaaat atgcgtttca ttgctgacgg caataatctg
    44281 ctctacctgc aaacgttggg caaacagaca ggccgcttcc atcacgaggc gtttgggaaa
    44341 tagcccgtgg caggctttcg tcgcattctg gatttcctgg tgtggaattt cccattttgc
    44401 gccttgcagt ccgccaataa acatcgtcct tttcccctga tattcacaca gggtaaaagt
    44461 gagctctgcc agaggaatac cttcgctgtt gcggaacagg atggtgctgt caccttcttt
    44521 atccattgag atcatcatgg tcagctcaag cgtgaactgc tcgccgtttt tgccttccag
    44581 cttcgccagt tgcagcccgg gggtattcaa atataagctg aattcttccg ccgacataca
    44641 cccgcggagt aacgcataat ggtaacgtaa cgcctccagc aattgcttac ggctaagatt
    44701 cgccgcaagg taagggcgat gtagacgtac aggcagtcgc ggctggcgcg ttaacaacac
    44761 attgagatta ggccagtggg aaagttcgtt catccactca acgcttaaac gcggcataat
    44821 caacgagcgc agcaaaaatt tctggcgaaa actacggcga tgccagaatt tacccggccg
    44881 acactgtcct cgtgccagac taagaaaaag tgacaggctg ctgagagatt cagatggcgt
    44941 aaaggtccgt tcagttagct gcgacatatt catgaaatca atggttatac atgacgtcga
    45001 tttaaccatt gcgtatctta accaaacatc aatagtgtga ttattaacgt aaattttagg
    45061 gttttgttga tatttcgttg aagttaatga cccggattgg catatggagt attcagaata
    45121 tttatgaaaa agcggaaaac cgtgaagaag cgttacgtta ttgcgatggt gatagtcatc
    45181 gccgggctga ttacgttatg gagaattctt aacgcacccg tgccgactta tcagacgctg
    45241 attgtgcgcc ccggtgattt acagcaaagc gtgctggcga ccggaaagct ggacgcgctg
    45301 cgtaaggttg acgtgggcgc gcaggtcagc ggtcagttga aaacgctgtc ggtggcgatt
    45361 ggcgataaag taaaaaaaga ccagctttta ggggttattg atcctgaaca ggctgaaaac
    45421 cagatcaagg aggtggaagc aacgctgatg gagctacgcg cgcagcggca gcaggcggaa
    45481 gcggagctga aactggcgcg ggtgacgtat tcccgtcagc aacgtcttgc acaaacgcaa
    45541 gcggtttcac agcaggatct cgataacgcc gcgacggaga tggctgtgaa acaggcgcaa
    45601 attggcacca ttgacgcgca aatcaagcgc aatcaggcct ctctcgatac ggctaaaacc
    45661 aatctcgatt acacccgcat cgttgccccg atggccgggg aagtcacgca aatcaccact
    45721 ctgcaaggcc agacggtgat tgccgcacaa caagcaccga acattctgac gctggcagat
    45781 atgagcacca tgctggtaaa agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg
    45841 gggcaaaaag cctggtttac ggtgcttggc gatccactga cgcgctacga ggggcaaatc
    45901 aaggatgtgc taccgacgcc ggaaaagatt aacgacgcta ttttctatta cgcccgtttt
    45961 gaagtaccca accccaatgg tttactgcgg ctggatatga ccgcgcaagt gcatattcag
    46021 ctcaccgatg tgaaaaatgt gctgacgatc cctctgtcgg cgttaggcga tccggttggc
    46081 gataatcgtt ataaagtcaa attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg
    46141 attggcgcac gtaacgatac cgatgttgag attgtcaaag ggctggaagc gggcgatgaa
    46201 gtggtgattg gtgaggccaa accaggagct gcgcaatgac gcctttgctc gaattaaagg
    46261 atattcgtcg cagctatcct gccggtgatg agcaggttga ggtgctgaag ggcatcagcc
    46321 tcgatattta tgccggtgag atggtagcga ttgtcggtgc ttcgggttct ggtaaatcga
    46381 ccctgatgaa tattctcggc tgcctggata aggccaccag cggcacctat cgcgtcgccg
    46441 gtcaggatgt tgccacgctg gacgccgatg cgctggcgca actgcgccgc gagcatttcg
    46501 gctttatttt ccagcgttac catttgcttt cgcatttaac cgccgagcag aacgttgaag
    46561 tacccgccgt ctatgccggt cttgagtgga aacagcggct gcttcgcgcc caggagttac
    46621 tgcaacgact ggggctggaa gaccgtacag agtattaccc ggcacagctt tcgggtggcc
    46681 agcaacagcg cgtcagtatc gcgcgggcat tgatgaacgg cggtcaggta attcttgccg
    46741 atgaaccgac cggcgcactg gacagccatt ctggcgaaga ggtgatggcg atcctgcatc
    46801 agctgcgcga tcgcgggcat acggtgatta tcgtcacaca cgatccgcag gttgctgctc
    46861 aggccgagcg ggtgatcgaa attcgcgacg gcgaaattgt gcgcaatcct cccgccattg
    46921 aaaaagtgaa tgctactggc ggaacggaac ctgttgtcaa cacggcgtct ggctggcggc
    46981 agtttgtcag cggttttaac gaggcgctga cgatggcatg gcgggcgctg gcagcgaata
    47041 aaatgcgtac tttactgacc atgctgggga ttattatcgg tattgcgtcg gtggtttcca
    47101 ttgtcgtggt gggcgacgcc gccaaacaaa tggtgctggc ggatattcgt tctattggta
    47161 cgaatactat tgatgtctat cccgggaaag attttggcga tgacgatccg caatatcagc
    47221 aggcgctgaa gtacgacgac ttaatcgcca tccaaaaaca accgtgggtc gcctcagcca
    47281 cacctgccgt ctcgcaaaac ctgcgcctgc gttataacaa tgttgatgtg gctgccagtg
    47341 ccaatggcgt gagcggcgat tattttaatg tctatggcat gactttcagt gaagggaaca
    47401 cctttaatca ggagcagctg aacggtcgtg cgcaggtcgt ggttctcgac agtaatactc
    47461 gccgccagct tttcccccat aaagcagatg tggttggcga ggtgattctg gttggcaata
    47521 tgcccgccag agtcattggt gtggcggaag aaaaacagtc gatgtttggt agcagtaaag
    47581 tgctgcgtgt ctggctgcca tacagcacga tgtccgggcg agttatgggt cagtcgtggc
    47641 tgaactccat aactgtcagg gtgaaagaag gctttgacag cgctgaggcg gaacagcaac
    47701 tcacgcgttt actttcactg cgccacggta agaaggattt ctttacctgg aacatggatg
    47761 gcgtcttgaa aaccgttgaa aagaccacac gtactttaca actgtttctg acgctggtgg
    47821 cggtgatttc gctggtggtg ggcggtattg gtgtaatgaa tattatgctg gtgtcagtga
    47881 ccgagcggac gcgggaaatt ggcattcgca tggctgtagg tgcgcgagca agcgatgttt
    47941 tgcaacagtt tctgatcgaa gccgtactgg tttgcctggt tggtggcgct ttgggaataa
    48001 cactgtcact gttaattgct ttcaccttgc agcttttctt acccggctgg gagattggtt
    48061 tttcaccgtt agcgctgctg ctggcgtttc tctgctcgac ggtcaccggg attttatttg
    48121 gctggttacc cgcacgaaat gcggcacgac tggatccagt agatgctctg gcacgggagt
    48181 aatttttgag ataaaaatgc cagccgatcg ggctggcatt ttgcctttag gatgtacaca
    48241 atgagacaga agagctatgc gactgccgct tctacttcga cgggcacaat aacactggcg
    48301 tgattgcctt ttggcccctg gtggacatca aactgaacgg attgtccagc ttttagcgtt
    48361 ctgtaaccat ccatctgaat ggtggaataa tgagcgaaaa tatcttcgcc gccgccttca
    48421 gggcagatga aaccaaaccc tttggcattg ttgaaccact taacagtacc cttttccatg
    48481 cttcgacatc cttcgcaaat cttatacaag taagatggaa taaaccgggg tcagagaggg
    48541 ggctgttcaa aacctcgcca actctagaaa tacaatttag agaattaggg cgagccgtca
    48601 agcatttgac aggggccagg gggcaggtat gaatcaaaaa tttgaagcag ttaacgctat
    48661 tgacaggaat gtgacagatg tcgctgatgc caacgataga tgatagttat ctatcatgtg
    48721 gagtagattg gtcaggcaaa taagctcttg tcagcggcag ggcgttctgc cgataaccgt
    48781 aaccgaagat gataactgac aatgggtaaa acgaacgact ggctggactt tgatcaactg
    48841 gcggaagaaa aagttcgcga cgcgctaaaa ccgccatcta tgtataaagt gatattagtc
    48901 aatgatgatt atactccgat ggagtttgtt attgacgtgt tacaaaaatt cttttcttat
    48961 gatgtagaac gtgcaacgca attgatgctc gctgttcact accaggggaa ggccatttgc
    49021 ggagtcttta ccgctgaggt tgcagaaacc aaagtggcga tggtgaacaa gtacgcgagg
    49081 gagaatgagc atccattgct gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg
    49141 ggggaggtgc ctatgctcaa tcaagaactg gaactcagtt taaatatggc tttcgccaga
    49201 gcgcgcgagc accgtcatga gtttatgacc gtcgagcact tgttactggc gctgctcagt
    49261 aacccatctg cccgggaggc gctggaagcg tgttctgtag atttggttgc gctccgtcag
    49321 gaactggaag cctttattga acaaaccaca cccgttctgc ctgccagtga agaggagcgc
    49381 gacacacagc cgacgttgag ttttcagcgt gtactgcaac gtgcggtctt ccatgtccag
    49441 tcctccggtc gcaatgaggt taccggtgca aacgttctgg tcgctatctt tagcgaacag
    49501 gagtcgcagg cggcatatct gttgcgcaaa catgaagtca gccgtctcga tgtggtgaac
    49561 tttatctctc atggcacgcg taaagacgag ccgacacagt cttctgatcc tggcagccag
    49621 ccaaacagcg aagaacaagc tggtggggag gaacgtatgg agaatttcac gacgaacctg
    49681 aatcagcttg cgcgagtggg cggaatcgac ccactaattg gtcgtgagaa ggagctggag
    49741 cgtgctattc aggttctctg ccgtcgccgt aaaaacaacc cgctgctggt gggggagtct
    49801 ggtgtcggta aaaccgcgat tgcggagggg cttgcatggc gaattgttca gggcgatgtg
    49861 ccggaagtga tggctgactg tacgatttac tctctcgata tcggttccct gttagcgggc
    49921 acaaaatatc gcggcgactt tgaaaaacgt tttaaagcgt tgctcaagca gctggagcag
    49981 gacactaaca gcatcctgtt tattgatgag atccacacca ttatcggtgc gggtgcagcg
    50041 tctggtggcc aggtcgatgc ggctaacctg attaaaccgt tgctctccag cggtaaaatc
    50101 cgcgtaattg gttcgacaac ctatcaggag ttcagcaaca ttttcgagaa agaccgtgct
    50161 ctggcgcgtc gcttccagaa aattgatatt actgaaccgt caatcgaaga aactgttcaa
    50221 atcatcaatg gcctgaaacc gaagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa
    50281 gcggtgcgtg cggcggtaga gctggcggtg aaatacatta acgatcgtca tctgccggat
    50341 aaagcgattg atgttatcga cgaagcgggc gctcgcgcac gtctgatgcc ggtaagcaaa
    50401 cgcaagaaaa ccgttaatgt ggcggatatt gaatccgtgg tggcccgtat tgcacgcatt
    50461 ccagagaaga gtgtttctca gagtgaccgc gataccctga aaaacctcgg cgatcgcctg
    50521 aaaatgctgg tcttcggtca ggataaagcc attgaggcgc tgactgaagc cattaagatg
    50581 gcgcgtgcag gtttaggtca cgaacataaa ccggtcggtt cgttcctgtt tgccggccct
    50641 actggggtcg ggaaaacaga ggtgacggta cagctttcga aagcgttggg cattgagctt
    50701 ctgcgctttg atatgtccga gtatatggaa cgccataccg tcagccgtct gattggtgcg
    50761 cctccgggat acgttggttt tgatcagggc ggtttgctga ctgatgcggt catcaagcat
    50821 ccacatgcgg tgctgctgct ggacgaaatc gagaaagcgc acccggacgt gttcaatatt
    50881 ctgttgcagg tgatggataa cggtacgctg accgataaca acggacgcaa agcggacttc
    50941 cgtaacgtgg tgctggtgat gaccactaat gccggggtac gggaaactga gcgcaaatcc
    51001 attggtctta tccaccagga taacagcacc gatgcgatgg aagagatcaa gaagatcttt
    51061 acaccggaat tccgtaaccg tctcgacaac attatctggt tcgatcatct gtcaaccgac
    51121 gtgatccatc aggtggtgga taaattcatc gtcgagttgc aggttcagtt ggatcagaaa
    51181 ggtgtttctc tggaagtgag ccaggaagcg cgtaactggc tggccgagaa aggttacgac
    51241 cgggcaatgg gcgcacgtcc gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc
    51301 gccaacgaac tgctgtttgg ttcgctggtg gacggcggtc aggtcaccgt cgcgctggat
    51361 aaagagaaaa atgagctgac ttacggattc cagagtgcac aaaagcacaa ggcggaagca
    51421 gcgcattaat ctgattgtct ggtaggttgg tgaagtccgt aatctcgtca ggggttacgg
    51481 actttttatt tatgggggga ggaggttcag accctttttt tgatgatgat ggtaagttat
    51541 tgataattag tgctgcgggt aggtaaggat aaaaaagggt ggcagcagga gattgagatg
    51601 gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc cgtacaggca
    51661 gcttagaaat tgccgcggat cctgtctgcc aataatgaca agttcactgc cgtacaggca
    51721 gataaaatgc gaaaaaaaag cccgtacttt cgtacgagct cttctttaaa tatggcggtg
    51781 agggggggat tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc
    51841 agccactcgg acacctcacc aaattgtttt tttgcctgac ctcatggggg gcaacggggc
    51901 gctactatag ggagttggaa taaaacggtc aagaagaatt tttatgataa ttattgtttg
    51961 ctcatactgt aaacaagttg tgcagtatat ctacatcgag acaagttacg gacttatact
    52021 tctaaagtac tccatacata tcacaaaata aaaaggccgg ctaaaccgac cttttactca
    52081 ttctttctct tcgcccatca ggcggtaaaa caatcagcga ctacggaaga caatgcggcc
    52141 tttgctcagg tcgtacgggg tcagttcaac agtcactttg tcgcccgtca ggatgcggat
    52201 gtagtttttg cgcattttac cggagatgtg tgcagtaacc acgtgaccgt tttctaactc
    52261 tacgcggaac atggtattag gcaacgtttc aagaacggta ccttgcattt caatattgtc
    52321 ttctttggcc atctaatcct ctggggtatc actaccgtaa tttgaaccgg caagataatg
    52381 ccgaagttct gtaaataagt aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac
    52441 attactccga aaacacacgg ctaagccgca ccaaaagcgc aacgtataag ggagcggtga
    52501 gataaacgat gggcgttacc tgacgcgaaa aattccttat cggcagcggg gtaatgagcg
    52561 taaccaactc tgcgaccgca attataacac tctggggaga aatgtgccga aaacattcat
    52621 tcttgtggtg aaaacaagca tcgaggtacc cagaaattat tcgacaatcg tccgaggcgc
    52681 atttgattga gataattaag gtaatcccgg cggggaattt cgcaggcacc aagcgatgct
    52741 gtgtgatcgt taaggacctg gcagtcgata agtttaccgc catgacggat aaattcatca
    52801 cagaagacca gaagcgccgt tttggacgca ttttccatcc ggctgaacat ggactcgcca
    52861 caaaatagtg ttccctgggc cacgccgtac ataccgccga caagctcatc ttcacgccag
    52921 acttcaatgg agtgggcatg accgagttcg tgaaggcgat ggtaggcttc gaccacgcca
    52981 cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc cttcgatgac ctgaccaaaa
    53041 gcgtaattca tcgtgacacg atagggtgag cgtttatgaa atcgcttcat actacggctg
    53101 atatgcagtg attctggcca taacaccgcg cggggatcgg gcgaccacca gaggatgggg
    53161 tcgccaggag aaaaccacgg gaaaatacca cgctgataag ccattaatag gcgcgcaggg
    53221 ctaagatcgc cccccagtgc cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa
    53281 gggaaggcta ttgaatggcg agaaagctga accaggcgca tgaccgcaaa actccacgca
    53341 agtcggatcg ttcaataata gcttacaaac cctgcttaaa ctggtaataa cgcccctgtc
    53401 tggcaagcag ttctgcgtga gtaccttgct caataatttg cccgttgtcc atcactatta
    53461 tttgttggaa acgagagagc ccgcgaagtc gatgggtgac cattaacacc gttttctcgc
    53521 acatcatttc tgcaagcaat tcaaggatct gactttcggt tgtggcatct aagccttctg
    53581 taggttcatc cagcaatacc agcggcgcat catgtaacag cgcacgggcg atagccagac
    53641 ggcgcagttc accaccggag agctggcgtc cgccttcacc taaccaactg ttgagacctg
    53701 catcctcgag cagcttctcc agaccaacgc gacgcaagat ctccgccaga gcctcatcac
    53761 tactaccagg cgaggcgagt aacagattat cacgcagcgt ggcgctaaac agatgcactc
    53821 gctggggaac aacgctgatg gtctgtcgta gagccgcttc attcaggctg gctatgggac
    53881 tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc gcgggtcagc agttgtaaca
    53941 gtgttgactt gccgcatccg gttcgcccga gaatcgcaat atgttccccg gcgtttacct
    54001 gaagataaat ccctttaagt gcctgttggg attgctccgg ataagtgaac tgaacatccc
    54061 gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt atcagggaag gtgactttcg
    54121 gtttttgatc cgttaaatca gtgatccgta cggcagaggc aatgacttgc cccagatgct
    54181 gaaatgcacc cgttactggt gccagcgctt caaacgcggc taacgcgcag aagacaaaca
    54241 gggcaattaa cgcgccgggt tgagcattgc cgccaacgcc gccagacgcc atccacagca
    54301 tcaggatcac cgctaacgcg ccaatgagca gcattatcgc ttgcgacaat gcggttagtt
    54361 cagattgacg gcgttgcgct tccagccatt gaatttctgt attctctaat tgcgtgcgat
    54421 aacgatcgct ggcaccaaaa atggtcagct cagcttgccc ttgcagccag gccgtcagtt
    54481 gttggcgata ctgcccgcga agatgagtca gattttgccc ggtgcttttt cccgcacgat
    54541 aaaacagtgg tggcatcagg aaaagcgtca gtaacataat gccgcccagc gtaaaggcga
    54601 gggtgaaatc aaggaaactt aacccgattg tcaccaccat aatcaccaca aaagcgccta
    54661 ccagcggcga gataacgcgc aggtaaagat gatcgagcgt atcaacatcc gccaccacgc
    54721 ggttgagtaa ttcgccctga cgatagcgcg ccagtcctgc aggggagagg ggcagtaatt
    54781 tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa agtcgcatcg tgacttacca
    54841 ggcgttcaaa atagcgcccg gcagtacggg tgattgctgc gccacgcacg cccgcagcgg
    54901 gtagcatata gttgaagctg tacagtccgg caacccccgc aaccgctgag gcggagagga
    54961 accagcccga aagtgtcaac agaccgatac tggcgagcag cgtgacaatt gccagcacga
    55021 taccaagact taacatccat ttatgacgtt tatacagtgc cagatagggt agcaaagcgc
    55081 gcatttaaat ctcctcctga cgatgggcca gtaatgtggc gaatgggcca ccagccacac
    55141 ttaattccgc gtaacgtcct tgctcaataa tctgaccatc ctgcattacc caaatgacat
    55201 cccagtcagc aagatcttct aactggtggg tgaccattaa cgttgtctgg cgcagagagg
    55261 cggcattcag cgcctccatt acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt
    55321 catccaacag taatagcgaa cagggattta gtaacgcacg ggccaccgcc acgcgctgcg
    55381 cctgccccac ggaaaggcgg gcagcctggt cgccaacagg cgtatcaacg ccttgtggca
    55441 ggagcggtag aaactcactg acccaggcgt tatccagcgc tgtttgtaac tcttgttcgc
    55501 tggcatcagg tcgcgccagt agtacgttat cccgtaatgt tgctgccggt aattgtgggt
    55561 tttgcccaac ccaggagaga tgtttacgcc atgattccgg tgataaatcg cgtaattcta
    55621 tcccgttgat tcgtagcgat ccctgatatg agagaaaacc agaaagcgcg ttcaacagtg
    55681 aacttttacc tgaaccgctg cgaccaacca acactgctcg ttggcctgct ggcaaagtaa
    55741 aattcagcgg tccggccagc gttttacctt ccggcgacgt gataaacaga tcctcggctt
    55801 caatggtcac cggatcggtc gatgctaatt ccgcttcacc gcgctgcgga tgggctagcg
    55861 gggtttccat aaacgttttc aaactgtcag ctgcaccaac agcctgggct ttagcatgat
    55921 aaaacgtacc gagatcgcgt aatggctgga aaaactctgg cgcaaggatc agggccagaa
    55981 aacccgcagc cagcgtcacg ccagtatcgt agtgaccaaa atccagctcg ccgaggtagg
    56041 aaaaaccaaa gtagaccgcc accagagcaa tcgacagcga ggtaaaaaat tcgagaatgc
    56101 cggaggataa aaacgccagc cgtagcactt ccattgtccg ttggcggaaa tcttccgaag
    56161 cagaacgaat actttcaatt tcagcttcac cacgaccaaa aatacgcaat gtttccatgc
    56221 cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag agcgagaaag ttacgtcggt
    56281 tagcatcggc agcccccatt ccaaccagcg ccataaataa cggaattagc ggtgcagtgc
    56341 ccagcagaat gagcgccgcg gcccagttag aggggaagat agccaccaca atcagcagcg
    56401 gcaccgacac tgccagcgcc atttgcggca ggtagcgtgc atagtaatca tgcatatcgt
    56461 caatttgctc gagtaccagc gtcgcccagc tccccgcagg tttaccctga atccacgctg
    56521 gccctgcttg ttgcagacgg tcgagaacct gacggcggat ggcaaagcgg atatgctgcc
    56581 cggcgtgata acccacccgt tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca
    56641 gaaacagtaa cgtaaaggga agcagcaggg cttcacgggg aatattctcc ataatcatat
    56701 gttgcagaat tcgcgccatg aaccaggcct gggcaatgat caatatgccg ctcacaaagc
    56761 ccagcagacg agaaatattc agccaacgtt gggagatgac gctttgctgt tttaaccagc
    56821 gggttaattc tttttgacgg gatttattca ttgcacgctt agcaggtgag ttatcagaat
    56881 tatttgcaga gcaatgttac aacggggaaa aaataaaggc gacccatagt cgcatggtgt
    56941 cgccttcttt acttttgtta ctgatttgta aaattatttt gcgtcagcta aaccgtcgag
    57001 gtagcgttcc gcatcaagag ctgccatgca gcctgtacca gcagatgtaa tggcctggcg
    57061 ataaatgtga tccatcacgt cgcctgcggc aaagacgcca gggatgctgg tctgggtggc
    57121 attaccatga atacccgact gtactttgat gtagccgttt tccagttcca gctgcccttc
    57181 gaaaatggca gtattcgggc tgtgaccgat agcaacaaac agaccggcaa cgtcgagtga
    57241 ctcgatatta tcggtgtttt gcgtatcgcg cagacgaacg ccagtgacgc ccatctgatc
    57301 gccggtcact tcttccagcg tacggttggt gtgcagaata atgttgccgt tctccacttt
    57361 atccatcaga cgcttaatga ggattttttc cgcacggaaa ccatcacggc ggtgaatcag
    57421 atgaacttcc gaagcgatgt tagacagata cagcgcctct tcaaccgcgg tattgccgcc
    57481 gccgatgacc gcaactttct ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga
    57541 aactccacgg cctttaaagg cttcttcaga gggcaggccg agatagcgtg cagaagctcc
    57601 ggtggcaata atcagcgcgt cgcaagtgta ttcgccgcta tcgccagtca gacggaacgg
    57661 acgattctgc aaatcaactt tgttgatgtg atcaaagatg atttcagttt caaatttggt
    57721 ggcatgttcg tgcatgcgct ccattaataa cggaccggtc agatcgtttg gatcgccagg
    57781 ccagttttcc acttccgtgg tggtggtcag ttggccgcct ttttccatgc cggtaatcag
    57841 cacaggttgc aggttggcgc gcgccgcgta gacagcagcg gtgtatcccg ccgggcctga
    57901 acccaggata agcagtttac tgtgtttggt cgtgcccatg agatccccat agttgttggc
    57961 agacaatggc aggattgtag ggaatttaca gacgtaaaaa aagagtatga cgattttgtt
    58021 aacaatttgt gcaatcggca gcatcgataa gcaggtcaaa ttctcacgtc attatcacct
    58081 ctgctactta aatttcccgc tttataagcc gattaaatga tgaataaacg cccctgttaa
    58141 tgaatatctg gcatgttgta ctaaaaatcg atgttttgct ttgacaatcc cctggtgttt
    58201 tgcgaaaaca ttcgaggaag aaaaaaaaca gtattcttat atgcgcataa ccatgcatgt
    58261 aaataccatg tttaccgtgc tagtgaaatc tacgtatggc gtggacagac gccattcgtg
    58321 atgtcgatag ctgccacaag gcaacggtct tctcaccgta gacccaggca ttgcgcgccg
    58381 tgaatcttca tgatttcggt ctatcgtgac gggtagcgac tctgaacagt gatgtttcag
    58441 ggtcagacag gagtagggaa ggaatacaga gagacaataa taatggtaga tagcaagaag
    58501 cgccctggca aagatctcga ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat
    58561 gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac tttccccaac gccgtgcctt
    58621 gagcgtgtgc gtcggctgga aagacaaggg tttattcagg gctatacggc gctgctgaac
    58681 ccccattatc tggatgcatc acttctggta ttcgttgaga ttactctgaa tcgtggcgca
    58741 ccggatgtgt ttgaacaatt caataccgct gtacaaaaac ttgaagaaat tcaggagtgt
    58801 catttagtat ccggtgattt cgactacctg ttgaaaacac gcgtgccgga tatgtctgct
    58861 taccgtaagt tactggggga aaccctgctg cgtctgcctg gcgtcaatga cacacggaca
    58921 tacgttgtta tggaagaagt caagcagagt aatcgtctgg ttattaagac gcgctaacac
    58981 ggaacaggtg caaaatcggc gtattttgat tacactcctg ttaatccata cagcaacagt
    59041 actggggtaa cctggtactg ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg
    59101 gagagccttt cttgagccag gaatacactg aagacaaaga agtcacattg acaaagttaa
    59161 gtagcggccg ccgccttctg gaggcgttgc tgatccttat tgtcctgttt gccgtctggt
    59221 tgatggctgc cttactaagc tttaaccctt cggaccccag ctggtcgcaa acggcctggc
    59281 atgaacctat ccataattta ggtgggatgc ccggtgcgtg gctggcagat acgctgttct
    59341 ttatttttgg cgtgatggct tacaccattc ccgtcattat tgtcggcggt tgttggtttg
    59401 cctggcgtca tcagtccagc gacgaataca ttgattattt tgccgtttcg ctacgcatca
    59461 ttggcgtttt ggcgctcatc cttacctcct gtggtctggc ggcaatcaac gctgacgata
    59521 tctggtattt tgcctccggt ggcgtcattg gcagcttact aagcactacg ctacaaccac
    59581 tgctacacag tagcggggga actattgcgc tgctctgcgt ttgggcagcg ggcctgacgc
    59641 tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact cggcggctgg attttaaata
    59701 ttcttacctt cgccagtaat cgtacccgtc gcgatgatac ctgggtcgat gaagatgagt
    59761 atgaagacga cgaagagtat gaagatgaaa atcacggcaa acagcatgaa tcacgccgtg
    59821 cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc
    59881 cgatggggcg gcaaacagac gctgcgttgt tctccggtaa gcggatggat gatgacgaag
    59941 agattaccta cactgcacgc ggtgtggctg ccgaccctga cgacgtcctc ttttcgggca
    60001 atcgtgcaac gcagccagaa tatgacgagt acgatccatt attaaacggt gcgccaatta
    60061 ccgaacctgt tgctgtagca gctgctgcta ccacggcgac acaaagctgg gctgcaccgg
    60121 ttgaacctgt gactcagacg ccgccagttg cctctgttga tgttccacct gcgcaatcta
    60181 cagtagcctg gcagcctgta ccggggccgc aaacgggaga accggttatt gctcctgcac
    60241 cggaaggtta cccacagcag ccacaatatg cgcagcctgc agtgcaatat aatgagccgc
    60301 tgcaacaacc agtacagcca cagcagccgt attatgcacc tgcagctgaa caacctgcgc
    60361 aacaacctta ctacgctcct gcggcggaac aacctgttca gcaaccgtat tacgcaactg
    60421 cggcggaaca gcctgcgcaa cagccgtatt acgcccctgc gccagaacaa gcggtggcag
    60481 gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga
    60541 ctgagcaaac ttatcagcag ccagccgctc aggaaccgtt gtaccaacag ccgcaacctg
    60601 ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccacgcgtc
    60661 cgccgcttta ctactttgaa gaagtggaag agaagcgagc ccgtgaacgc gaacaacttg
    60721 cggcctggta tcaaccgatt ccagaacctg ttaaagaacc agaaccgatc aaatcttcgc
    60781 taaaagcacc ttctgttgca gcagtacctc cagtggaagc cgctgccgcc gtttctccgc
    60841 tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc
    60901 cagtcttcag tctggcaaat agcggtggac cgcgtccgca ggtcaaagag gggattggcc
    60961 cgcagttgcc acgaccgaaa cgcatccgcg tgccaactcg tcgtgaactg gcgtcttacg
    61021 gtattaagct gccctcacag cgtgcggcag aagaaaaagc ccgtgaagcc cagcgtaatc
    61081 agtacgattc tggcgatcag tacaacgatg atgaaatcga tgcgatgcag caggatgaac
    61141 tggcacgtca gttcgcccag acgcagcagc aacgctatgg cgaacagtat caacatgatg
    61201 tgcccgtgaa cacagaagat gcagatgctg cggcagaggc agaactggct cgtcagtttg
    61261 ctcaaactca acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc
    61321 tggatgattt tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgc
    61381 tgtttacgcc aattgttgaa cctgtacagc agccgcaaca accggtagca ccgcagcagc
    61441 aatatcagca gccgcaacaa ccggttgcac cgcagccgca gtatcagcag ccgcaacaac
    61501 cggttgcacc gcagcagcaa tatcagcagc cacaacaacc ggttgcacag cagccgcagt
    61561 atcagcagcc acaacagccg gttacacagc agccgcagta tcagcagccg cagcagccgg
    61621 ttgtaccaca gccgcaatat cagcagccgc aacagccagt tgcgccacag ccgcaggata
    61681 ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga
    61741 cgccactgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
    61801 cctttgcgct tgaacaaatg gcgcgcctgg tagaagcgcg tctggctgat ttccgtatta
    61861 aagccgatgt tgtcaattac tctccggggc cagttatcac tcgctttgaa ttgaacctgg
    61921 caccgggcgt aaaagcggcg cgcatttcta acttgtcacg tgaccttgcc cgttcacttt
    61981 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    62041 taccgaataa aaaacgacaa accgtttatc tgcgcgaggt tttggataac gccaaattcc
    62101 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    62161 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaagt
    62221 ctgtcggggt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    62281 gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcacc
    62341 tgttaacgga agttgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta
    62401 acgagatgga gcgtcgttat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt
    62461 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    62521 agccgggtga tagtatggat gcgcagcatc cggtgctgaa aaaagaaccg tacatagtgg
    62581 tgttggtgga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga
    62641 tagcacgtct ggcgcaaaaa gctcgtgccg cgggtatcca cctcgtactg gcaacgcagc
    62701 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    62761 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggt gcggaatcac
    62821 tgctgggtat gggggatatg ctctactctg ggccgaactc cactttgccg gtacgtgtcc
    62881 atggtgcttt tgttcgcgat caagaagttc atgccgtggt gcaggactgg aaagcgcgtg
    62941 gtcgcccaca gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggcgcgggcg
    63001 gtttcgatgg cgctgaagag ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    63061 aaaaacgcaa agcatcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    63121 cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    63181 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcatcgt aggccggata
    63241 aggcgcggta gcgtcgcatc cggcaatctg gtaactgaaa attcagtatt ttcttctttc
    63301 ctcaagctga ttattagcct ggaatagaga gtagagggag ctcccggtcg ggagtgacgt
    63361 aatttgagga ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt
    63421 agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag
    63481 cagcttccac gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga
    63541 aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata tgactcaacc
    63601 tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga
    63661 gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc
    63721 ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt
    63781 tctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga
    63841 tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcagct
    63901 gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg
    63961 cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt
    64021 tttcggataa tacttttcaa cccctggccg cgcgtatgcg gccagaaaat ttagcacagt
    64081 atatcggcca gcaacatttg ctggctgtgg ggaagccgtt gccgcgcgct atcgaagccg
    64141 ggcatctgca ttctatgatc ctctgggggc cgccgggtac cggcaaaaca accctcgctg
    64201 aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg
    64261 tgaaagagat tcgcgaggcg atcgaacgcg ctcggcaaaa ccgcaatgca ggtcgccgca
    64321 ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc
    64381 cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg
    64441 agcttaattc ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgagtacag
    64501 aggatattga gcaagtacta actcaggcga tggaagacaa aacccgtggc tatggtggtc
    64561 aggatattgt tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg
    64621 cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg
    64681 gtaagcgggt cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct
    64741 ttgataacaa aggcgatcgc ttttacgatc tgatttccgc attgcataag tcggtacgtg
    64801 gtagcgcacc cgatgcggcg ctctactggt atgcgcgaat tattaccgcc ggtggcgatc
    64861 cgttatatgt tgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc
    64921 cacgggcgat gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg
    64981 aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg
    65041 ctgtctacac tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgtccg gattatgacg
    65101 tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg
    65161 aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc
    65221 cggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg
    65281 gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt
    65341 aatgttatcg ttgcggtaat gttgttaatg tatccctgtg gtcgcaggct gtggccacat
    65401 ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg
    65461 agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc
    65521 tgggcgctct tgaggagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg
    65581 agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc
    65641 ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg
    65701 atgctttaca ggctgaaatt cgcgatatcg cgctaaccat ccctaacctg cctgcagatg
    65761 aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagtcgctgg ggcaccccgc
    65821 gtgagtttga ctttgaagtt cgtgaccatg tgacgctggg tgaaatgcac tctggcctcg
    65881 actttgcagc cgcagttaag ttgactggtt cccgctttgt ggtaatgaaa gggcagattg
    65941 ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct
    66001 acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc
    66061 tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca
    66121 gtaactatgc gctgatccca acggcagaag ttccgctgac caacctggta cgcggtgaaa
    66181 tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgt ttccgttctg
    66241 aagctggttc atatggtcgt gacactcgtg gtctgatccg tatgcaccag ttcgacaaag
    66301 ttgaaatggt gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgaccg
    66361 gtcatgcgga aaaagtcctg cagctgctgg gcctgccgta ccgtaaaatc atcctttgta
    66421 ccggcgacat gggctttggc gcttgcaaaa cttatgacct ggaagtatgg atcccggcac
    66481 agaacaccta ccgcgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta
    66541 tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg
    66601 gttctggtct tgctgttggt cgtacgcttg ttgcagtaat ggaaaactat cagcaggctg
    66661 atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaatggactg gaatatattg
    66721 gctaataccc aatttttctg aatctaaaaa gcgcctgcgg gcgctttaag tagaggttta
    66781 atcatttgaa aataaatgga ttttattttt aagtgtccat ataacgtcca tctttgaatg
    66841 caaaaagccc acagcttcta tgctgtgggc tttgtttttt ggtgtcctca tgagtgaggg
    66901 aaccaaaaag aaaacccgca gtttttacgc tgcgggcttg ttgttcacgt caactggaaa
    66961 tagggtgcct tttcgacctt taccctggca accgattgac ggggaagttg ccttccccgt
    67021 cgcggtttcc ttactgctta cactgtaaga acgccgcaaa ctccgctccc cagaaactca
    67081 tccgtatttc acacagcgaa ccgtgcagca tccagatgat gaggattgcc gtcacgcaga
    67141 atgtgatggc cgtaagcgat ttttgcgaca tagcgcttgc tcctttttcg gggaggcgct
    67201 aacctatcac ttgctgaggg tagatggtta gggcctcggg ttaacgttaa agttgactcg
    67261 gggcctttcc acatctggcc ttcgggtatt ccctccgacc atcagccgaa aggcacccgc
    67321 acgtaatcta tcgctttttt gttactccgg caattctgcc tgttaattct gagataaagg
    67381 caaactcatc tgattgtttc ccctgtgtga agctggcaga tcatgccacg ggataccttc
    67441 tgaagagtga acgccggagg cgtgttttga tgtgaattta tggaaagctt ccagtgttga
    67501 gaagcatacg ccgcattcca gattgttaca ctggtaatac ttttgccgca cggtgtttga
    67561 atcattttcc ggacgactgg tgcggatacg ggcagatgcg ccacaaagcg gacaacggaa
    67621 catagcgacc tcccttaacg tggtgctgcc tattctaagt tgctcactct gtttccgcta
    67681 tccattccgg gatttttgcc tcaagctcaa gctgcgtggt aaaaccgctg ttatcaatgg
    67741 tgtgctcggc ttttgcaatg atccagtcct gattatcaat ctcgctttta aatcctgtta
    67801 ccgtgccatg catttcgggg tagagttctg cacgtccacg cgccagcgtg atggagaatg
    67861 atgcggctcc gcgttgtagt tgctgccact ttgccgccgc tgcgcgtctt gctgcctgct
    67921 cgttctgata agtcttgcgt aacacaaaca cattgccttc cgcgccttcc atataatcac
    67981 cttcgcggct gctgcttttc tcctttttgg gttttggcgg tttacggcgt ttcacgctga
    68041 cttttttctt tttcccgtaa ttaagatcaa gccagtaagc gcgtaccccc gtatacgctt
    68101 cgcggtcagc aatgcggaac tgatggcgat cgccgctgct gcgtgtgatg gcaaacgatg
    68161 gcaacggctg gccctgtgcg ttcacgccac cgccgggcat gatgaataac agattaccgc
    68221 tttttaccgt ggtgattgcg cccagcattt ccgccatgcg cgtaaggaag gacatgtcgc
    68281 tttcttcggt ctggtcggcg tggtcgattt cgatatccat cagcatttcg ctgatttgcg
    68341 gtttcagacc ataccgatga gcgatggcgg ataccacacg ctcaacggtc acatcatgcc
    68401 aggacacctc acgtttaacg ttaaattcat cccgaaaatc tgcgcttctg gctgaaacag
    68461 tcagcctgtc cggcggtcct tcgtgagcga tttcatcaac aatgtaagtg cctttttctg
    68521 tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt ggcggtagct
    68581 gcaactgacc atccgcatca tccagcgtga tggtgagctg gtccgcctca aatccccggt
    68641 tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc tgacagcgtt ttaccctccg
    68701 cgagaatatc aaaatccggc attttcacgg ggtctgtgcc ctgactgagc aattgcatgg
    68761 tggtgtcggt catctgctcc ctccctgtgc ggcatggtcg catgtgcgtg cggagggggt
    68821 tactgctttt tgttgtcgcc gtggcgggag aacggcgcag gggtgagatt acgcgcgtgg
    68881 tgggtgatga ttgttgccga atcatttaac ggatacaagg ggctgaagct atgagtgaaa
    68941 ctcgttttca tggtgcccgt gtcacggaaa gtaccgacct ggtaacagcg attaatgatg
    69001 ttgattccag cgttatcggt atcgtggcaa cggcggatga tgcggatgcg gagctgttcc
    69061 cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa tgcgggacta
    69121 cggggacgct ttatcgtgcg cttaaggcta tcgcagacca ggtgagcaca aaagtgatcg
    69181 tcgttcgcgt ggctgaacac aaagaagaag gcggtaggac gcaggatcaa ctggttatcg
    69241 gtggttctga atctgacggc agctatacgg ggatgtatgc gctgcttgtt gcagagcagg
    69301 atgaaagcat cggataccgt ccgcgtattc tggccgcgcc ggagctggac acggaggcgg
    69361 tgacaaaatc cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat gccacatgtc
    69421 atggttgtaa cacgatggct gaggcgatta cctaccgcca gaaattcaac gaacgtgagg
    69481 tgatgctctt atggcctgac ttcatcgcct acaacccgaa aagtggcaaa aacgaaacgt
    69541 tccccgcgcc tgcttatgcg tgcggccttc gtgcgtacat tgaccatgag cagggctggc
    69601 acaaatcgct gtccaacgtt ccggttaaaa atgtgctggg gatgtcgagg catgtgttct
    69661 ggtcgttaca ggccgaagac agtgatgcca acagcctcaa caacaaagaa atcacgacca
    69721 ttattcgtcg taacgggttc cgcttctggg gcaaccgcac accggaaacg aacgcctaca
    69781 tctttgaggt gtatacccga accgcacagg tgctggctga ttcaattgcg gaagcgcagt
    69841 ttgaaaccat cgacagtcca ctgacgcctg cgaacgtgaa ggatgttatc agtgccatca
    69901 gggcaaaatt ggattcgctg gttactgccg ggaaactgat tggcgcggag tgctggtatg
    69961 acgtggtgga taacggcacc acggatttac gtcaggggcg tgtgcgtatt cgctacaaat
    70021 atacgcccgt tcccccgctg gaagacatgg agctttacca gacgtttact gatgaattct
    70081 ttggtcccgc atttgcggtg ctgggaggtg cctgatggct gtaccaaaac atcttcgctt
    70141 ttttacgttg tttgtggatg gtgaaaacga agtgggtaaa gtgacatccg tcacgccgcc
    70201 caaactgacg cgcaaaaccg acagctatcg tggtggtggc atgatgggag cagtaagtat
    70261 tgatctcggt ctggacgact ccgcgcttga tgcgagcttt gtcatggggg gagctgttcg
    70321 tgcactgttc cttaagtatg gcggcacgat tgacggcacg ctgctgcgtt ttgcgggtga
    70381 atattacacc gatgcagaaa gcgatctgta tgaaatcgag atgcgcgggc gtgtgacgga
    70441 aattgatatg ggggaggcca aacagggcga agccacatca cacacttacg ctgtcaaaaa
    70501 cacctactac aagctgagcg ttaacgatcg cccgctgtgg gaaatcgacc tgttgaacca
    70561 catctaccgg aaggacggca aggacattgt gcctgaccgc attcgttccg cgcttgggct
    70621 tggctgataa gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa aggagacaac
    70681 tgatgaaaga tatcgatact gaaacccgga ataacaccgt agcggatgat gtgacgtcag
    70741 gtgaggatat ggctgtcgaa cgtggtgtaa aactttcccg gccaattgag cgtggtgccg
    70801 aaaaaatcac atatgtggag atcaccgggg ctattgagca ggcgggatcc ctgcgtggtc
    70861 tgtcgctgtc tgatgtgctg aatctgaaag cggataccat gttcacgctg ttgcctcgcg
    70921 tgacctcgcc acgactggat gaagtgatga ttaaaaaaat gtcgtcacgc gattttattc
    70981 agttgtgcgc tgtggctgta aattttatga gcgagccaga ctctggcgcg aagagcgtgc
    71041 aggagacggc agcgtaatca ccctggtgtg ctttgagcac atcgaagatc tggtggcgga
    71101 tattgccgcc attttcaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt
    71161 ggttagctgg cgtgagcggg cggcacttcg cagcgggaat gcagataatg aagactcttg
    71221 acatccgggt cgctttcagc gccgttgaca ggctgacccg gcctgccgaa aacgcccgcc
    71281 gcctgatggg gcagtttggt gactccattc agcgaacgca gggggcgatc aaaaatctcg
    71341 agcgtcaggc gcgttcattt gagcgcgccc gcgacgctgt cagtaaagcg gatgcgggta
    71401 tcgtgaaagc acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc
    71461 tcagcgaaaa acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg
    71521 aatcgcgcac acgggaaatt cagaaaatgc gggagcttgg cggagaactg aaacgccacg
    71581 gcatttccct gacaggcagc gataacacca tccagcaggc catcagacgc accgaacaat
    71641 acaacaacca gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcag gctcgtgagc
    71701 ggtattcgcg cgcgcaggaa accgcgggaa aactgaaaac aggtggtgcg ctggcaattg
    71761 gtgcggcagc ggcgggcggc tatgctgccg ggcgtttttt gcagcccgcg atcgggttcg
    71821 gcaaagagat gtcccgcgtt caggcgctga cgcgaatcga caaaaacagc ccgcagttta
    71881 aggcgctgcg tgagcaggcg ttaaaacttg gctctgaaac acagtttact gcgagtgatg
    71941 ccgccagtgg gcagagcttt ctggcaatgg ctggttttac tccgcaggcc attcaggccg
    72001 cattgcccgg tgttcttaat atggcgctgg caggtggcgt cgaactcggc gagacggctg
    72061 atataggctc caatatcctc acacagttca acctgacagc cgatcaaatg gaccgggttg
    72121 gcgatacgct gacagcggca ttcacccgga ccaatactga tttacgcgcg ctgggcgaaa
    72181 ccatgaagta taccggtccg gttgccgcaa aacttggtat cagtcttgaa gaagcggcag
    72241 ccatggccgg gatgcttgcc aataatggtc ttcgtggaag cgatgctggt acggccatgc
    72301 gcgcaagtct gtcccgcctt gcatcaccgc caaaagctgc ggctgatgcg ctgaaagagc
    72361 tgggggtgtc agttgctgac gccagaggca aaatgcgccc gatggaggat gtgctgcttg
    72421 atctctataa ggcgacacaa aaatacggac aggtggacca ggtttccttc ttcaaggaca
    72481 tcgccggaga agaggcgttc gttggtttgc agacgcttgt tgcggcggct ggttcaggag
    72541 agctgcaaaa actgaccaga gaattgcagg gggcaagggg agaggccgat cgcgttgcaa
    72601 aagtaatggc cgataatctt gatggggacc tgaaaaatct cgacagcgca tgggaaggtc
    72661 ttcgtattcg catcagtgat ctggttgacg gtccgctgcg ttctgtcacg cagtggctca
    72721 cgcgggtgct tgaaaaaatc acctcgctgg cgcaggccca tccggtactg acgcgccagc
    72781 tactgatagc aggcggtgcg ttgctggcaa tgactgcaac ggttggctcg ttgtcgctgg
    72841 ctattggtgt gcttgctggc ccgctggcaa aactgcgtct tggcttttcc ctcctgaccg
    72901 gttcaatgaa tgctgtcaga ctcctgccag cactatgggg aatggtgacg ggttccgttt
    72961 cgttactggg gggcgctatc ggggcgttgt tcagtccggt cggccttatc gtggctgcgc
    73021 ttgccggagc tgccgttctc atctggaaat actgggatcc catcagggca ttttttgccg
    73081 gggtgttcag cgggattatg gaaaggctga ccccgttgcg cgaaaccttt gaacggtttg
    73141 gtcctgtttt tgacgcaatc gggagtggga tcagccaggt gtttaactgg tttaaatcgc
    73201 tgctgtctcc gatggagtcc agcaaggaaa ctctggataa atgtaccagt gctggcgaga
    73261 tattcggtaa cgttcttggc ggtgcgttac aacttgttct gacacctgca aaaatgttgc
    73321 tggatacgct ggcgtggata cttgaaaagc ttggtgtgct tccggatgaa gcggaaaggg
    73381 cgcgcaagaa aatcgaagac gcacagcgtg cggccattct tcaggacaag gttgccttgc
    73441 ttcaggggga cctggcgaaa atcaatccgc cgaagcctgt ggaaaatggc aatggcaccg
    73501 gaggtgataa ccccaaagac aataaaccgc tcacagacag caataccggt acgctgcgca
    73561 gactcagcaa aattgctgat aacacaggta agctggttga ggagacgaaa aaacgcattg
    73621 gccccggcga tattgtcttt aaaaacctgc cccgagcact tgctgttcgt ggggagtggc
    73681 aggagcggaa gattgcgcag gtcagtaagc ctgcccccgc aattaatatc acacccgtgg
    73741 tcccggctcc gctgcctccg gcgctggtcc ctgttgttgc ggccagctcc cgcccggtgg
    73801 cggaggctat acgatctcca gtggcatcag ttcctgtaac ttcccgtaac cgggagcctg
    73861 ttgcctccgg atttggtggt gaaattcatg ttcatctgca taacgttgtt acgcagaatc
    73921 cccgcgaact ggcgaaactg gtcggtgaaa tggtcagggc agaaatggaa cggcgcgccc
    73981 gtgccgggcg tggcagtttt tacgataaag attgaggagt catggccatg atgatgatct
    74041 acggcatgtt tgtttttgag ctgcgcacgc tgccgcatca gcagttacag caaaacaaaa
    74101 gctggcggca tgtgaaaaat gaacgcgtta accgttcagc aagctggcag tatatcggtg
    74161 caggtgatga tcgcatcgtt ctttctggtg tgctttatcc tgaaattaca ggtggcgaag
    74221 tgtcgctgtc gctgctgacc acgcaggcgt atacaggacg accctggcct ttgattgatg
    74281 gcgtcgggca gatttacggc atgtatgtcc tgactgaaac gaatacgacc cgctccgagt
    74341 ttgatcgcta cggtaaggcg aaaaagatag aattttcact gactcttgaa cgctgtgatg
    74401 aggatttgcg ggagcgcctg caatcctcat cgttcagtga tatgctgtcc ggcttcaaag
    74461 ataaggtgac atcatctctt aacagcgcgg ccagttcagt taaagggctg ttctgattta
    74521 acacaaaaac cgctaatggt cagattagcg gttttcattt tcctgagtct gcccggttgt
    74581 ttcttcagtc tgtatatcgc ctacagggtg ataacgataa atcgtcgatg tgccaatgcc
    74641 gtaaattatt gccagttgtt ttctgtcgtg cccgtttttg attagccttg ctatttgctc
    74701 atgctgttct tttgtcagct tcggtcgacg tccgcctgtg cgcccccttg cgcgcgctgc
    74761 cgccagcccg gccagtgtac gttcaacaat taattcacgt tccatttcag ccagggcacc
    74821 catcacgtgg aagaaaaaac gccccattgg agaagatgtg tctatgctgt cggtcagact
    74881 gcgaaaatta atccctcgct cccgtagttc cccgacgaga gaaatcagat gtttcatgct
    74941 tcgcccgagg cgatccagtt tccagacaac cagcgtgtca cctttttgaa ggcgctttaa
    75001 agcgcgtttt aatcccggtc ggtctgtctt tgtcccgctt aatttatctt caaatatttg
    75061 ttcacatcct gcacaaacaa gagcgtttcg ttgcaggtct gtattctggt catttgttga
    75121 tacccttaca tagccaatca gcacactgaa tctcccgtcc aaaagcgcaa atcatgccat
    75181 gcaggccaga aacggccatt at
//
LOCUS       DACADA010000005        65715 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.005, whole genome shotgun sequence.
ACCESSION   DACADA010000005 DACADA010000000
VERSION     DACADA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.005"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            167..1405
                     /locus_tag="ISB17_000318"
     CDS             167..1405
                     /locus_tag="ISB17_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1978300.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            1399..2634
                     /gene="preA"
                     /locus_tag="ISB17_000319"
     CDS             1399..2634
                     /gene="preA"
                     /locus_tag="ISB17_000319"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAP1978301.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(2877..3887)
                     /gene="mglC"
                     /locus_tag="ISB17_000320"
     CDS             complement(2877..3887)
                     /gene="mglC"
                     /locus_tag="ISB17_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAP1978302.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(3903..5423)
                     /gene="mglA"
                     /locus_tag="ISB17_000321"
     CDS             complement(3903..5423)
                     /gene="mglA"
                     /locus_tag="ISB17_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAP1978303.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(5484..6482)
                     /gene="mglB"
                     /locus_tag="ISB17_000322"
     CDS             complement(5484..6482)
                     /gene="mglB"
                     /locus_tag="ISB17_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAP1978304.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(6762..7802)
                     /gene="galS"
                     /locus_tag="ISB17_000323"
     CDS             complement(6762..7802)
                     /gene="galS"
                     /locus_tag="ISB17_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAP1978305.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(7944..9101)
                     /locus_tag="ISB17_000324"
     CDS             complement(7944..9101)
                     /locus_tag="ISB17_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAP1978306.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(9118..9786)
                     /gene="folE"
                     /locus_tag="ISB17_000325"
     CDS             complement(9118..9786)
                     /gene="folE"
                     /locus_tag="ISB17_000325"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAP1978307.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            10044..10880
                     /gene="yeiG"
                     /locus_tag="ISB17_000326"
     CDS             10044..10880
                     /gene="yeiG"
                     /locus_tag="ISB17_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP1978308.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(10912..12903)
                     /gene="cirA"
                     /locus_tag="ISB17_000327"
     CDS             complement(10912..12903)
                     /gene="cirA"
                     /locus_tag="ISB17_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAP1978309.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(13195..14664)
                     /gene="lysP"
                     /locus_tag="ISB17_000328"
     CDS             complement(13195..14664)
                     /gene="lysP"
                     /locus_tag="ISB17_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAP1978310.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(14869..15750)
                     /locus_tag="ISB17_000329"
     CDS             complement(14869..15750)
                     /locus_tag="ISB17_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978311.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15849..16898
                     /locus_tag="ISB17_000330"
     CDS             15849..16898
                     /locus_tag="ISB17_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAP1978312.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            16972..17829
                     /gene="nfo"
                     /locus_tag="ISB17_000331"
     CDS             16972..17829
                     /gene="nfo"
                     /locus_tag="ISB17_000331"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP1978313.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            17833..18921
                     /locus_tag="ISB17_000332"
     CDS             17833..18921
                     /locus_tag="ISB17_000332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1978314.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(19189..20130)
                     /gene="rihB"
                     /locus_tag="ISB17_000333"
     CDS             complement(19189..20130)
                     /gene="rihB"
                     /locus_tag="ISB17_000333"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAP1978315.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            20260..20958
                     /gene="yeiL"
                     /locus_tag="ISB17_000334"
     CDS             20260..20958
                     /gene="yeiL"
                     /locus_tag="ISB17_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAP1978316.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(21025..22275)
                     /locus_tag="ISB17_000335"
     CDS             complement(21025..22275)
                     /locus_tag="ISB17_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP1978317.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(22369..23307)
                     /locus_tag="ISB17_000336"
     CDS             complement(22369..23307)
                     /locus_tag="ISB17_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAP1978318.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(23295..24236)
                     /locus_tag="ISB17_000337"
     CDS             complement(23295..24236)
                     /locus_tag="ISB17_000337"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAP1978319.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(24692..26383)
                     /gene="fruA"
                     /locus_tag="ISB17_000338"
     CDS             complement(24692..26383)
                     /gene="fruA"
                     /locus_tag="ISB17_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAP1978320.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(26400..27338)
                     /gene="fruK"
                     /locus_tag="ISB17_000339"
     CDS             complement(26400..27338)
                     /gene="fruK"
                     /locus_tag="ISB17_000339"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP1978321.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(27338..28468)
                     /locus_tag="ISB17_000340"
     CDS             complement(27338..28468)
                     /locus_tag="ISB17_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAP1978322.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            28836..30017
                     /gene="setB"
                     /locus_tag="ISB17_000341"
     CDS             28836..30017
                     /gene="setB"
                     /locus_tag="ISB17_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAP1978323.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(30014..30268)
                     /locus_tag="ISB17_000342"
     CDS             complement(30014..30268)
                     /locus_tag="ISB17_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP1978324.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            30423..30995
                     /gene="yeiP"
                     /locus_tag="ISB17_000343"
     CDS             30423..30995
                     /gene="yeiP"
                     /locus_tag="ISB17_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAP1978325.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            31218..32684
                     /locus_tag="ISB17_000344"
     CDS             31218..32684
                     /locus_tag="ISB17_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1978326.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            32802..33788
                     /locus_tag="ISB17_000345"
     CDS             32802..33788
                     /locus_tag="ISB17_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP1978327.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            33827..34540
                     /gene="lpxT"
                     /locus_tag="ISB17_000346"
     CDS             33827..34540
                     /gene="lpxT"
                     /locus_tag="ISB17_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAP1978328.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            34952..35518
                     /gene="mepS"
                     /locus_tag="ISB17_000347"
     CDS             34952..35518
                     /gene="mepS"
                     /locus_tag="ISB17_000347"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAP1978329.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            35699..37255
                     /locus_tag="ISB17_000348"
     CDS             35699..37255
                     /locus_tag="ISB17_000348"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP1978330.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            37337..39151
                     /locus_tag="ISB17_000349"
     CDS             37337..39151
                     /locus_tag="ISB17_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1978331.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKISDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            39152..40246
                     /locus_tag="ISB17_000350"
     CDS             39152..40246
                     /locus_tag="ISB17_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAP1978332.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            40246..41271
                     /locus_tag="ISB17_000351"
     CDS             40246..41271
                     /locus_tag="ISB17_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1978333.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            41273..42862
                     /gene="yejF"
                     /locus_tag="ISB17_000352"
     CDS             41273..42862
                     /gene="yejF"
                     /locus_tag="ISB17_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAP1978334.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(42866..43210)
                     /locus_tag="ISB17_000353"
     CDS             complement(42866..43210)
                     /locus_tag="ISB17_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978335.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(43544..44734)
                     /gene="bcr"
                     /locus_tag="ISB17_000354"
     CDS             complement(43544..44734)
                     /gene="bcr"
                     /locus_tag="ISB17_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAP1978336.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(44762..45457)
                     /gene="rsuA"
                     /locus_tag="ISB17_000355"
     CDS             complement(44762..45457)
                     /gene="rsuA"
                     /locus_tag="ISB17_000355"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAP1978337.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            45607..47367
                     /locus_tag="ISB17_000356"
     CDS             45607..47367
                     /locus_tag="ISB17_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP1978338.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            47492..47776
                     /gene="rplY"
                     /locus_tag="ISB17_000357"
     CDS             47492..47776
                     /gene="rplY"
                     /locus_tag="ISB17_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAP1978339.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(47915..48922)
                     /gene="yejK"
                     /locus_tag="ISB17_000358"
     CDS             complement(47915..48922)
                     /gene="yejK"
                     /locus_tag="ISB17_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAP1978340.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            49104..49331
                     /locus_tag="ISB17_000359"
     CDS             49104..49331
                     /locus_tag="ISB17_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAP1978341.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            49351..51111
                     /gene="pbgA"
                     /locus_tag="ISB17_000360"
     CDS             49351..51111
                     /gene="pbgA"
                     /locus_tag="ISB17_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAP1978342.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            51186..51262
                     /locus_tag="ISB17_000361"
     tRNA            51186..51262
                     /locus_tag="ISB17_000361"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51220..51222,aa:Pro,seq:ggg)
     gene            complement(51373..52316)
                     /locus_tag="ISB17_000362"
                     /pseudo
     CDS             complement(51373..52316)
                     /locus_tag="ISB17_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            52636..53283
                     /gene="narP"
                     /locus_tag="ISB17_000363"
     CDS             52636..53283
                     /gene="narP"
                     /locus_tag="ISB17_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAP1978343.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(53318..54370)
                     /locus_tag="ISB17_000364"
     CDS             complement(53318..54370)
                     /locus_tag="ISB17_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAP1978344.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(54367..54924)
                     /gene="dsbE"
                     /locus_tag="ISB17_000365"
     CDS             complement(54367..54924)
                     /gene="dsbE"
                     /locus_tag="ISB17_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAP1978345.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(54921..56864)
                     /locus_tag="ISB17_000366"
     CDS             complement(54921..56864)
                     /locus_tag="ISB17_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP1978346.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(56861..57340)
                     /gene="ccmE"
                     /locus_tag="ISB17_000367"
     CDS             complement(56861..57340)
                     /gene="ccmE"
                     /locus_tag="ISB17_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAP1978347.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(57337..57546)
                     /gene="ccmD"
                     /locus_tag="ISB17_000368"
     CDS             complement(57337..57546)
                     /gene="ccmD"
                     /locus_tag="ISB17_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAP1978348.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(57543..58280)
                     /gene="ccmC"
                     /locus_tag="ISB17_000369"
     CDS             complement(57543..58280)
                     /gene="ccmC"
                     /locus_tag="ISB17_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAP1978349.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSILGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(58322..58984)
                     /gene="ccmB"
                     /locus_tag="ISB17_000370"
     CDS             complement(58322..58984)
                     /gene="ccmB"
                     /locus_tag="ISB17_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAP1978350.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(58981..59598)
                     /gene="ccmA"
                     /locus_tag="ISB17_000371"
     CDS             complement(58981..59598)
                     /gene="ccmA"
                     /locus_tag="ISB17_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAP1978351.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(59617..60219)
                     /gene="napC"
                     /locus_tag="ISB17_000372"
     CDS             complement(59617..60219)
                     /gene="napC"
                     /locus_tag="ISB17_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAP1978352.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(60229..60678)
                     /gene="napB"
                     /locus_tag="ISB17_000373"
     CDS             complement(60229..60678)
                     /gene="napB"
                     /locus_tag="ISB17_000373"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAP1978353.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(60675..61538)
                     /gene="napH"
                     /locus_tag="ISB17_000374"
     CDS             complement(60675..61538)
                     /gene="napH"
                     /locus_tag="ISB17_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAP1978354.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(61525..62220)
                     /gene="napG"
                     /locus_tag="ISB17_000375"
     CDS             complement(61525..62220)
                     /gene="napG"
                     /locus_tag="ISB17_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAP1978355.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(62227..64713)
                     /gene="napA"
                     /locus_tag="ISB17_000376"
     CDS             complement(62227..64713)
                     /gene="napA"
                     /locus_tag="ISB17_000376"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAP1978356.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(64710..64973)
                     /gene="napD"
                     /locus_tag="ISB17_000377"
     CDS             complement(64710..64973)
                     /gene="napD"
                     /locus_tag="ISB17_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAP1978357.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(64963..65457)
                     /gene="napF"
                     /locus_tag="ISB17_000378"
     CDS             complement(64963..65457)
                     /gene="napF"
                     /locus_tag="ISB17_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAP1978358.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        16220 a        17534 c        16135 g        15826 t
ORIGIN      
        1 gaataattat tacttcactc aataagctgc ggatataaca ttctgaaccg cagctatttg
       61 tgaatccttt cacagtttaa atttccccac gcgcatagcc ttaatatcag cacgcaatat
      121 ttatttagcg ttcgccttaa ttactcatag cattaaggaa gatcacatgc cgcaacaaaa
      181 ttatctggat gaactcactc cggcttttac gcctttactg gcgattaaag aagcctctcg
      241 ctgtttatta tgtcatgacg ctccctgtag tcaggcttgc ccggcacaga ccgatccggg
      301 gaaatttatt cgctcaatct actttcgtaa ttttaaaggc gctgccgaga caattcgcga
      361 aaataatgcc ctcggtgccg tttgtgccag agtgtgcccg acggagaaat tatgccaaag
      421 cggttgtaca cgtgccggtg ttgatgctcc cattgatatc ggtcgcttac agcgttttgt
      481 tactgatttt gaacaacaaa ctgggatgga aatttatcag cccggtacta aaacgctcgg
      541 caaagtcgcc attattggcg caggtcctgc cggattgcag gccagtgtga cgctgacaaa
      601 ccagggttat gacgtcacga tctatgagaa agaagcacaa cccggtggct ggttgcgtaa
      661 cggcattccg caattccggt taccgcaatc ggtgctggat gcagaaatcg cccgtataga
      721 aaaaatgggc gtgaccatta agtgcaataa cgaaatcggt aaaacactca ccctggagca
      781 gctgaaagcg gaaaaccgcg cggtactggt caccgtaggg ttatcgagcg gttccgggtt
      841 atcgctgttt gagcatagcg acgttgagat tgccgttgac ttcctgcaac gtgcacgaca
      901 ggcgcaaggc gatatcagca ttcaacaaag cgcattaatt atcggcggcg gtgatgtcgc
      961 gatggacgta gccagcacgc tgaaagttct cggctgtcag gcggtaacct gcgtagcgcg
     1021 tgaagagtta gatgagtttc cggcaagcga aaaagagttt accagcgcca gagagctggg
     1081 cgtttcgatc atcgatggat ttacaccagt agccgtggaa ggcaataaag tcacctttaa
     1141 gcatgttcgt ttaccgggtg aactaacgat agcggcggac aaaattattc tcgctgtagg
     1201 tcaacatgcc gaacttgctg ctttttccgc actggaaccg caacgtaaca ccatcgaaac
     1261 gcaacattac cagacccgcg acccgcaagt ctttgctgct ggcgatattg ttgagggtga
     1321 caaaaccgtg gtctatgcag tgaaaaccgg gaaagaagcc gccggggcga ttcatcacta
     1381 tttagaggga gcttgctcat gttaacgaaa gatctttcga ttactttttg cggcgtgaag
     1441 tttcccaacc cgttctgcct ttcttcttcg ccggtaggca actgctatga gatgtgcgcc
     1501 aaagcctacg acacaggttg gggcggcgtg gtgtttaaaa cgatcggttt ttttatcgcc
     1561 aacgaagtct cgccgcgttt tgatcatctg gtgaaagaag ataccggttt tatcggcttc
     1621 aaaaatatgg agcagattgc tgaacatccg ttggaagaga atctggccgc cctgcgtcgg
     1681 ctgaaagaag attacccgga caaagtattg atcgcttcaa tcatgggcga aaatgagcag
     1741 caatgggaag agctggcgcg tctggtgcaa gaagctggcg cggatatgat cgagtgtaac
     1801 ttctcctgtc cgcaaatgac ttctcatgcg atgggtagcg atgtcgggca aagcccggag
     1861 ctggtagaaa aatattgtcg ggcagtaaaa cgtggttcca cgctgccaat gctggcgaag
     1921 atgacgccga atattggtga tatgtgcgaa gtggcgctgg cggcgaagcg cggcggcgca
     1981 gatggcattg cggcgattaa caccgttaaa tccatcacca atatcgatct taatcagaaa
     2041 attggtatgc cgatcgttaa cggtaaatcg agtatttccg gatattccgg taaagcggta
     2101 aaaccgatcg ccctacgctt tattcagcaa atgcgcaccc atccagaact gcgcgatttc
     2161 ccgatcagcg gtatcggcgg cattgaaacc tgggaagatg cggcggagtt tttattgctc
     2221 ggttcagcga cgttgcaggt gaccaccggc attatgcagt acgggtatcg gatagtggaa
     2281 gatatggcaa gcgggttgtc gcattatctc gccgatcaag ggtttgattc gttgcaggag
     2341 atggtaggtc tggcgaatca caatattgtc ccggcggaag atttagaccg cagttatatt
     2401 gtctatcccc atatcaatct tgataaatgt gttggctgtg gacgctgtta tatttcctgt
     2461 tacgacggcg gtcaccaggc gatggaatgg agcgagaaaa cccgcacacc gcattgtaat
     2521 accgagaaat gcgtgggttg tctgctttgt ggtcacgtct gcccggtagg ttgtattgat
     2581 cttggggaag tgaagtttaa gaaaggcgag aaagaacacc cggtaacgtt gtaaaaccag
     2641 cgacgcatcc ggcatttagt tcctgtcgcc agatgcggcg tgaatgcctt acccggcata
     2701 caaaacaata taaatcagta tgttgtgctc attgtaggcc ggataaaacg cgccagcgtt
     2761 gcatccggca tttggctcct gtcgccggat gcggcgtaaa cgccttatcc ggcctacaaa
     2821 accacacaga tcatgatgtt atgtgcgttt atgaacgaat gcggaccaca ttcacatcat
     2881 ttcttacgcg cgtatttcag tgaatccagc gccacggcaa agataataat tgcccctttg
     2941 atgatgtact gccagtacgg gtttacgccg atgtacgtca ggccatagtt gatgacggta
     3001 aaaataatca ccccggtcac cacgccaatc accgtcccta caccgccgct gaacgatacg
     3061 ccgcctacca cgcacgctgc gatagcatcc agctcataca tgaagccgag gttgttggtg
     3121 gcagagccga tacgtccggc ttctaacatc ccgccgaagg catagaacac accagacaac
     3181 gcgtagatca tcagcaggtt caggccgacg ttgacaccag atactttcgc cgcttccggg
     3241 ttaccgccaa tggcaaaaat gttcttgccg aagcgggttt tgttccataa cacccagaca
     3301 aacaccaccg caatcaacgc atagaaggtg atgtaagaga gacggaaact ccccagcgca
     3361 ataaagcctt gggcaaaagt ggagaaacca ctgtcaaaac cggaaattgg cgacgccccg
     3421 acaaagtcgt aatagagcga gttaatgccg tagacgataa tcatcgtacc gagcgtggta
     3481 atgaacggcg tcacgttgag ataggcgata atcaagccgt tgatcaaacc gatcaccgca
     3541 ccaatggcac agacaatcag aataaccagc gcaatcggca tcgtcgccat ttccgggaac
     3601 actttgttgg cgttatccat ggactgcaat aacgtcgctg ctactactgc cgccagcccc
     3661 acctggcgac ctgcggaaag gtcagtcccc tgggtgacaa ttaaccctgc cacaccaagc
     3721 gcgataataa tacgcaccga tgactgggtg agaatattac ttaagttcaa cagacttaaa
     3781 aatgttgggt cctggaaaat aataatcgcc agcaaaacta aaagaacgac gtaaataccg
     3841 ccctctttca ggtaagtaag aaaacttttc ttatttaacg cactcatggg aagcccctaa
     3901 tcttaaaggt gcaaagacgc aagacgcaga atttcgcttt gcgttgttgt ttttgtatcg
     3961 acaattccgg aaacgagacc attgctcatg accagaatac ggtctgttat ccctaacaac
     4021 tctggcattt cggaggagat aataataatc cccttgcctt tcttcgccag ttcggcaatt
     4081 aactgataaa tctcaaactt agccccaaca tcaataccgc gcgtcggttc atcgagcatt
     4141 aatatttctg gttgcgttaa cagccagcga ccaataatca ctttttgctg attaccaccg
     4201 gagagcgaac caatttgcgt ccgatgcccc ggtgttttta cccgcatcga atcaatcacc
     4261 cactgggtat cgcttttcat ccgcgagtta tccagtaaac caactttatt tttatagttg
     4321 cggatattgg aaattaacga gttaaagcca atatccagat aggcataaat tcccgttgag
     4381 cggcgctctt cagttaccag tgcaaatccg tggtttatgg cttcgttggc attatggtta
     4441 ttgatctttt tgccgtgcaa cgtaatggtg ccagccgatt tctcgcgaat accaaataac
     4501 gtctcaacaa tatcggtacg tttcgctccc accagaccgg caataccgag gatctcccct
     4561 ttatgcagat caaacgagac atcgcgaatc gacggctggc gcagcgacgt caggttacgc
     4621 acttccagaa tcacttcgcc cggcttgttt tctttgtcag ggaaacgctg gttaagagaa
     4681 cgcccaacca tcatggcgat gattttgtcc atcgtcagtc ctgccagcgg ttcggtagcg
     4741 atccactgac cgtcgcgcaa tacggtaact tcatcacata actggaagat ttcttccatt
     4801 ttatgcgaga tataaacaat accgcagccg cgctctttta atttacgaat aatagtgaac
     4861 agatgattga cctctttttc ggttaacgaa gaagtcggtt catccataat cacaattttc
     4921 gcgttatagg aaaacgcttt ggcgatttcg atcatctgca tttgcgaaac agataatgta
     4981 ccgacgcgcg cacgcggatc gatatcaata tccagttcat caaaaatcgc tttggtttcg
     5041 cggtacattt tgtcctgatc gacaaacatg cctttggtgg gatatcgccc cagccacatg
     5101 ttgtccatca ccgaacgttg taataccagg tttaactcct gatgtaccat cgaaatacca
     5161 ttttccagtg cttctttggc ggaatggaaa tcgatctctt taccctggaa taaaatggtg
     5221 ccggagtctt tttgataaat accaaacagg cattttaata atgtcgattt tcctgcaccg
     5281 ttttccccca ttaatgcatg gatagaatgt ggccggactt ttaagttaac gttatcaagt
     5341 gccttaacac caggaaaaga cttgttgata ccgctcattt ccaacaagta ttcacctgat
     5401 gacggagtcg ttgagctgac catataattt taccttgttg gtcatacaat aagggcgcag
     5461 taaacgactg cgcccaatca gtcttatttc ttgctgaatt cagccaggtt gtctttatct
     5521 acgccaacat aaggtacgcg gaccactttg ttgtcgattt tccagttggt gccatcagcc
     5581 gcacctttgc catcggccag gtttttcgcc agatcaaagg tcgctttcgc ctggttgtta
     5641 gcatcgttca gtacggtgcc cgccagtgca ccggatttca ccagcgccag cgcttctggc
     5701 agcgcatcga cgccaaacac cggaatgctg gacttgttgt gtgctttcag tgcttctacc
     5761 gcgcccattg ccatcgcgtc gttgttggcg ataaccacct cgattttgtt ggcgttcggg
     5821 ccagacagcc aggcgtccat cttatctttc gcctgagcgg tatcccacat agcggtatct
     5881 aactgtaact gttcagtttt aatgcctttg tcgttcaatt ctttaatcac gtaagtggta
     5941 cgtgcttctg catccggatg ccccggctca cctttcagca gtacgaattg aatctgaccg
     6001 tctttgttca gatcccaacc ctgattcgcc gcccagtgtt tagcaatcaa atcgccctga
     6061 ataatgccgg actctttgga gtcagtgcca acgtagtagg ctttgtcgta gctatccagc
     6121 gccttacgag acggttcttt gttgaagaaa accaccggca cgttttgccc acgcgctttc
     6181 tcaatcaccg tacccgcagc tgccgggtca accaggttga ttgccagtgc cttcacccct
     6241 ttcgccagca atacgtcgat ctgatcgttc tgcttggact ggtcattctg agaatcattc
     6301 atcagcagct gaacatctgg cgcggctttc gcatcttgct caatagcctt gcgcactaca
     6361 gacataaagt tatcgtcgta cttatagatt gttacaccaa tgcgagtatc agcagcgtgt
     6421 gcagcggcac cgaataacat gctggccatc acagcagaca gggttaacac cttcttattc
     6481 atggtatctc cggtttttct tatgcagggt agtgcttgag ataaatgctc ggcggggcag
     6541 tagagttaat gaagtgttac tgaacgccga agctcacttt ttaaaattcg ttcttccatg
     6601 cccggtaacg ctccagaaaa cggctttaat tgttgttatg acgctattac ctcggtgaaa
     6661 gtgattattc actgttacat atgggttaca acgttaaaac ggtgcaatca tagccatcac
     6721 attgttaaga tactgtgaaa tcactcacag attgaaagcg gttacatcgc ctgatttgtt
     6781 gagttagtga tcgccgccgc attctggcgt gttgcgacag aatggcgacg cactaacgtc
     6841 ggcataaaac agtggctggc acgaggatca atattgtctg ctgccccctg caaggccagt
     6901 tcggtagcta atttagccat tgaagcaatg ggataacgca cggtcgttaa ttgcgggtcg
     6961 gtgtaacggg cgatgggaat atcatcgaaa ccgatgattg agagatgtag cggaatcgca
     7021 atgccattat cttttaatgc tgtcagtgcg ccagcagcca tattgtcgtt ataagcaaat
     7081 acagcggtta gttgtagatt gcgccccagc agttcaacca tcgccgcctc accgcccggc
     7141 atgtccggcg taccagtgcc aatccagctt tccggcggaa taatatcctg ctctttcaac
     7201 gcactcatcc agcctgcttt acgcatggcg tcatcttcaa tgccgtggct ggaagaaaga
     7261 taaccaatac gttgatgacc gttattcagc aacatgcgcg tcgccattcg ggcaccgctg
     7321 agattatcca ggcaaacgca acgatgggcg taccccggca caacgcggtt gattaacacc
     7381 ataccaggaa tgttatccat aaattgtgcc agttcatcgt cactcaatgc ttttgagtga
     7441 acaatcaacg cattacaacg ctggcgaatt aacacctcaa tggcgtgacg ctctttttcc
     7501 gcttcatgat agctattgcc gattagcacg tatttctgat gctgctgggc gaccagatcc
     7561 accgctttta ccagcgcgcc gaaaaaagca tcagaaacgt ccatcaccac cacgccaatg
     7621 gtgtcgctaa cctgagttgc cagcgcctgg gcattggcgt ttggccgata atccagctca
     7681 ctcacagctt tcattactgc ttcacgcgtg tcggcactga ccagcgtgct gttattgagc
     7741 acccgggaaa ccgttgccac agagacgcca gcctgacgcg ctacatcacg aatggtgatc
     7801 atattcacta cctgttatga gattgcagta aatgactgct tgctggcggc tattttgtca
     7861 gcactgaata caggacttcg tgaatcgagt cacagcaatg gaaacggtta cagccgtttt
     7921 gttaacgaat gtgatccaga tcgttatctt gatgttttag atattgtcgg tccggcagca
     7981 cgcaaagtta actgacgcca gagccattcc accggcccct ggcggaagta acgcagccag
     8041 ataacagaga agaagatatt cgccagccat accggaataa caaacgccag cagctccaga
     8101 cggtcaaact gcataaacaa actgaggtgg taaaaaagcg tagtacaaat cagcgtttgc
     8161 aatagatagt tggtcagtgc catccgtccg acacaggcga ttgccagcac cagcttaaag
     8221 cggcttaatt gcggccagaa gccataaaac agcgacgcat agccaatcgc ctgaaacggc
     8281 gcactcagtt cccgcggcat ttgaagtaag aaggcacacc agcgatacgc ccagtccagc
     8341 tgccattgca gggcgatggc aggaaggtta atgatcaccc caatcgccac cagcacaaaa
     8401 ccagtacgac gatagtgacg taagctgaac tgccctttca gccagccgct gcgcatcagc
     8461 gccgcaccaa tgagcatcat ccctgccagt tgccagccat actgtgcgcc cagtgccagt
     8521 aaactgttgc caacaccatc ggcacgatta ttgatcgctt caacaccacc gtgaagcttc
     8581 cagtattttt catacaaaat agccgatgca tccggcgtcc aggcgcggct ggtctggctg
     8641 tcggaaatca gccccaataa cagcaaaacg ccaagcccca ccagataaag catgacgcct
     8701 gtattaaaca ggctttttac cgatggcgca tcgcgcacca gccgccagca gattaagccc
     8761 accagtccgt aagccagcag aatatcgccg tcccagaaca ataaaccgtg aataaagccc
     8821 agcaagacta acagcgttaa ccgcgactgg atccagcgtc tgccacgggg cagcaacatt
     8881 tgcaggcccg caccaaacag cagcgcaaaa agcgtgagga atttcacctg gccgatgaga
     8941 tcgagaaatg cccaggtcca tgcatcctgc ggcgtaatag cgccgtacca ggcgggattg
     9001 agataagccg cctttggtag cccaaaggcg ctgatgttta atagcaggat ccccagaatg
     9061 gcgacgccgc gaacaaaatc gagcgtgacg ttgcgctcca tggctcctgc cttttaatca
     9121 gttgtgatga cgcacagcgc gcagaaactc gtggcgcgta ttctgactgg atttgaacaa
     9181 tccaccaaga gaggtcgttg ttgtggcact ggttgcgtcg cggatgccac gcgctttcac
     9241 gcagtaatgc accgcgtcga tagagacagc cacgttattg gtgcccagca gcgtttgtag
     9301 cgcaatgaga atttgctgcg tcagacgttc ctgcacctgc ggacgctggg caaagaactg
     9361 cacgatgcgg ttaatttttg acagaccgat caccgaatct ttcgggatat aagccaccgt
     9421 cgctttgcca tctatggtaa caaaatggtg ttcacaggtg ctggtcaggg tgatatcgcg
     9481 cacggtgacc atttcatcga ccttcatttt gttttcaatg agggtgattt tcgggaaatt
     9541 ggcgtaatcc agaccggaga aaatttcatc gacatacatt ttagcgatgc gatgcggcgt
     9601 ttccatcaaa ctgtcatcag ccaggtcgag attcagcagc tgcatgattt cggtcatatg
     9661 accagcaata aggcttttgc gcgtttcgtt atccatttca tgcacgggcg ggcgcagcgg
     9721 tgtttccagt cctcgcgcaa ctaacgcttc atgaaccagg gccgcttctt tactgagtga
     9781 tggcatttat gatttctcct gcaggtgtga cgcctccgcc ctgcgtgggg gcaaagttat
     9841 taagctgatt tacagcctga ttattgtgcg tgaggcggcg cacataatcc agtattcaca
     9901 gcgataatta ttgtaattgc cgctgccttt catcagcaga tgttaaaaca tcgttatgca
     9961 aatacggaag tgaaagttac tcacagcaca ttgaataaac ggtatgatga agaaattgca
    10021 aacaacacaa caaggagcca cgcatggaaa tgctcgaaga gcaccgctgt tttgaaggct
    10081 ggcagcaacg ctggcgacac gactccagta ccttaaactg cccgatgacg ttcagtatct
    10141 ttctcccacc acctcgtgat cacaccccgc caccagtgct gtactggctt tccggattaa
    10201 cctgcaatga cgagaacttc accaccaagg cgggtgccca gcgcgtggcg gcggaactgg
    10261 ggattgtact ggtaatgcca gacaccagcc cgcgcggcga acaggttgcc aacgacgatg
    10321 gctacgattt aggccagggc gcggggtttt atcttaacgc cacgcaacca ccgtgggcga
    10381 cgcattacca gatgtatgat tatctgcgcg atgaattacc ggcactgatt cagtcgcaat
    10441 ttaatgtcag cgaccgctgc gctattagcg gtcattccat gggtggtcac ggcgcgctga
    10501 ttatggcgct gaaaaatccg ggtaaataca ccagcgtttc cgcctttgcg ccaattgtga
    10561 atccgtgcag cgtcccgtgg ggaatcaaag cgtttagcac ttatttaggt gaggacaaaa
    10621 atgcatggct ggaatgggac agttgcgcgt tgatgtatgc cagtaacgcg caggatgcaa
    10681 tcccgacgct tattgatcag ggtgataatg atcagtttct tgctgaccaa ctgcaacctg
    10741 cagtactggc agaagccgcg cgccagaaag cgtggccgat gacgctgcgt attcagccgg
    10801 gatatgatca cagttactac ttcatcgcct cttttataga ggatcacctg cgcttccatg
    10861 cgcagtattt actgaagtga aagtccgccc ggttcgccgg gcatcttctc atcagaagcg
    10921 ataatccact gccataaagt aacgacgtcc gtcttcgtta tagctgtagt cgtcacgact
    10981 gagatccttg tcgccaaggt tcagcacgcc tgcacgcagt ttgacgtctt tagtcacctg
    11041 ccaggccgcg ccggtattcc agatggtata accgcccggt gttttagccg tcgcgctgtc
    11101 ggcgcgtttc tgcccggtat agtgaccaga aacatagaat gaccagtctt ccagcgccag
    11161 cggtttccag tccagcgtac cgttagcagt atggaacggc agatcggata gcggtttgtt
    11221 ttcgccgttg ctgacatcac gaccatcgtt gtaggtgtag ttgatcgaca gtttccattc
    11281 atcgttgaac ggaattttca gttcggtttc cacgccctga atacgtgctt tgttaacgtt
    11341 gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac cgacaaagtt
    11401 ttggtagccc ggtgcagcgt tgacgtcaga cgtacggcta atgctgatac gatctttcac
    11461 atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt cttcgcccat
    11521 gtagtaaagc cccagttccc aactttcgct ggtttctggt ttcagatccg ggctacccac
    11581 aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga
    11641 aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata
    11701 aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt ccatacgcac
    11761 gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa acagcgcgta
    11821 ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat cgctaagttt
    11881 gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt
    11941 gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt
    12001 gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt
    12061 atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg
    12121 atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc
    12181 aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc
    12241 ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt
    12301 cattcccagc acgccgtcaa ttaatggtcc gctggtaaag aactgaccgt tataggtatc
    12361 accgcgatcg cgatgttcct gaacggtggt atcgacggta acggtgcccg accatttctg
    12421 accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac cgtacagcga
    12481 cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag
    12541 atcgaaatca ttgtggcgga agacggcatt gcgggagtta acgcgtttac cgtcgacaag
    12601 aatcagggtg tagctgctgt ccagaccacg aatacttacg cccttacggt tatccccttc
    12661 gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt
    12721 tcgctgcagg tcttcctggg taatgacgct gatactggcg ggagcatctt taaggttttg
    12781 ttcaacggaa gatgcagtga caaccatcgt ttcgccatca tcatcgaccg ctaacacagg
    12841 ccatgcacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa
    12901 cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca catcttcttt
    12961 acgtcccctt gcgtacggca gcatcgcggt ggacttattt gtatgcaggt gatcatccca
    13021 gaaagccgga acagcctctg atttgtcagc cttttttgat tgcggcgtaa cgataatgca
    13081 aacgataaca attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
    13141 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
    13201 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
    13261 cttatagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
    13321 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
    13381 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
    13441 gttaatgtcg tgtccctgca atacgtaccc gcgacggaaa cgatagtggc taatggcaat
    13501 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
    13561 ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg tcgcatacag
    13621 cgcattacgc ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg ctttaccgtc
    13681 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ccgctgacag
    13741 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
    13801 gaacaccagg gtgaacggac tgacgctgat gtctttaaca tcgttacgca gcaggctcgg
    13861 atcggtgtac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
    13921 tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
    13981 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
    14041 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
    14101 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
    14161 aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccgcgaac
    14221 agagatgtag ttcaacagga agataacgcc gaggaacaac gcactccaga tccagcccgg
    14281 tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaggtcaa cggcaatagt
    14341 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
    14401 gttctgaccg tacgtggcaa acgaaccgga aaccggcata tatgcagcca gttcaccgag
    14461 actggtcatc aggaagtaaa ccatcaggcc aatcagcata tacgagagca atgccccgcc
    14521 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
    14581 gccaatggca atcatcgtca ggtgacgcgc cttcaattca cggcgtaagc ccggcgcttc
    14641 tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat gaggcgcgat
    14701 tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac accttcatga
    14761 tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca tgctgagggc
    14821 gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcacctt aacgcggtac
    14881 attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt gtttttgccg
    14941 atgatgtata cgccacaacg tacgcatcag gcgcggcaga gggaccgcaa cttcacttaa
    15001 tgtgcctgcc tgcaattgat cttcaatcac ccgtcgcgac aggcaactaa ttcccaaccc
    15061 atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt
    15121 cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac cgcgttcacg
    15181 caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag ccaacggcga
    15241 agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt
    15301 gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat
    15361 cacgtcctgg ctattcccaa cgctaagttc aatcggcaac tgcggataat catggcgata
    15421 acgggcgata actgcaggta gaatgtagtt accgatggta ctactggcat agatacgaat
    15481 cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcagtgccaa
    15541 cgctcgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc
    15601 aaacagttga acgccaagct gcccttccag gtcggtcaag gctgcgctca ctgctgattg
    15661 cgacaacgcc agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa
    15721 aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat
    15781 aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg
    15841 agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc
    15901 cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg
    15961 cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag
    16021 gcaacaccat ttacccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc
    16081 aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg
    16141 ccgatgtcgg tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc
    16201 tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg
    16261 gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagccg gtagtgaaag
    16321 cagaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcga
    16381 ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg
    16441 gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca
    16501 tcagcccgga tgcagaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg
    16561 ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctgggacg aacagcggcg
    16621 agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtcgtt gccatcttta
    16681 actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc
    16741 tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag
    16801 ccggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg
    16861 gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct tgctcctgtt
    16921 aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac
    16981 attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa
    17041 atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg
    17101 ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca ctacacatcg
    17161 gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt tgctgaagct
    17221 ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca gctggggctt
    17281 tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt
    17341 gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt caccgcggtg
    17401 attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg
    17461 attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac ctgccatgct
    17521 ttcgctgccg ggtatgattt gcgtactcct gccgaatgcg agaaaacgtt cgctgatttt
    17581 gcccgtattg taggctttaa gtatctgcgc gggatgcacc tcaacgatgc gaaaagcacc
    17641 tttggcagcc gcgttgaccg tcatcatagc ctcggcgaag gcaatatcgg tcatgatgcg
    17701 ttccgctgga tcatgcagga cgaccgtttc gacggcattc cgctgatcct tgaaaccatc
    17761 aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg
    17821 gtggcctgat cgatgaacga cagggaaaaa cagattctta aaattttacg gcgtaacccg
    17881 ctgattcaac agaatgaaat cgccgatatt ctgcaaatca gccgctcacg tgttgccgct
    17941 catattatgg atttgatgcg caagggtttg attaaaggca aaggatatat ccttaccgag
    18001 caggattact gtgtggttgt gggtgcaatc aatatggata ttcgcggaat ggcggatatc
    18061 cgttacccgc aagcagcctc tcatccaggt tctgttcact gttccgcagg cggcgtcgga
    18121 cgtaatatcg cgcataacct ggctctgctt ggtcgggatg tgcatttaat ttcagctatt
    18181 ggcaacgatt tttacggcga gacattactg gaagaaacgc gccgcgcggg cgtgaacgtt
    18241 tcaaactgca ttcgtctgca tggacacagc actgcaactt atctcgctat cgctaataag
    18301 caggaagaaa ctatcctggc aatcaatgac acacacattc tgcaacagct aacgcctcaa
    18361 ctgctcaata cttcacggga cttgattcgc catgctggtg tggtgctggc tgattgtaat
    18421 ctcacacctg aagccctgga gtgggtcttt accattgctg atgaaattcc gatgtttgtc
    18481 gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg gatccacact
    18541 ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc aaccgattaa agacgacaac
    18601 gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat ttttgtttat
    18661 ctgaaagatg aatctgtatt ttgcagcgat aaggatggag aacaattttt gctgaccgca
    18721 ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta
    18781 tatagctttc ttgaaggata cagtttccgc gacagcgccc gttttgcgat agcctgcgcg
    18841 gcaatttcgc gtgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt
    18901 tcattagtgc caatggtgta acaatgttgc cggattcgac gcttgaccgc atcttatccg
    18961 gctcataaat tgcgcactac gctaaaccaa taaagaatcc tgcaatagta gcactcatca
    19021 ggttactgac agcaaaaacg caatagccag caataccacc attcccagaa cacttctcat
    19081 gacatccata gttattatct cttcatgtca ttttgggcga cggctctctg cccatcataa
    19141 atagccgggc ctgcatcttg cgatactgac ccggcttgtt atcaacactt aatgggtttt
    19201 gatgtagccg cgtacgcact cttcgactaa tccccagaac cagtctgtat caatagtgat
    19261 gccgactttg gtattggcgg gcttgccaag aacgcccaac tcgtcgcaga cggtacgccc
    19321 atagcaaggg ccactgttaa cgtccacttc gacgtacatc tcctgggttt taatgccatc
    19381 agggttaatc aaataaccga tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta
    19441 gttttcgaac tgcgttttga gagtgaagtt catgatgtcg ctgaacagct ctcccgcagg
    19501 gccgccagcc ctttccatcc gagcaatcac gtccggggtg caaacggtct ggttggtaag
    19561 atcgaggccc atcatcacta atggaacgcc ggaggtgaac accacgcgtg cggcttccgg
    19621 gtcagcaaag atattgaact cagcagatgg ggtgaagttg ccagtaccat aagcgccgcc
    19681 catcagaaca atttcacgga ttttcggcag gatcgcgggt tgcatacgca ttgccaccgc
    19741 gatgtttgac aacggaccaa ccggcaccag agtgatatcg ccatcgcttg ccataagggt
    19801 atcgatgata tatttcaccg catgagtgtt ttctgcctgg cgggtcagcg gctcgaatac
    19861 cgggccatcc agtccggttt caccgtgaat attatcggca acaatttgtt gacgcataat
    19921 gggctgcggc atccccgcat aaaccggaac attaatctcc agtttctggc aaacattcag
    19981 gccattaatt aatgttttat cgagggtttg attacccgct acaatggtga tacctaataa
    20041 atcaatcgca ggatgtttcg ccgccatcat tatagcaata gcatcatcat gacccggatc
    20101 acaatccaga ataatttttc tcttttccat tgtttatttc ctctgtttcc agttgcgtta
    20161 ttgtttctac agcaaagaaa attcgacctg gccgatgatt gaatcttaac aacagcgtac
    20221 gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat
    20281 gatcttaagc agcaattgat gagtgaatct gcgtttaagg attgcttttc aacggatgtt
    20341 tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa
    20401 cagccgtcct ggctgtttta cctgacgcga ggccgcgcca ggctttacgt cacgcttgct
    20461 aatggtcgcg tgtcgctgat cgatttcttt gccgcgccct gttttattgg cgagattgag
    20521 ttaatcgaca aagaccatga accgcgtgca gtgcaggcta ttgaagagtg ttggtgcctt
    20581 gcgctcccta tgaagcatta ccgcccgctg ttattaaacg acacgctatt tttacgaaaa
    20641 ctctgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa
    20701 tcatttccgt tagtgaatcg cctggcagct tttatattac tctcgcagga aggtgatctt
    20761 tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta
    20821 tatgttctcg cgcaattcat taatgacggt ttattaatta aaagcaagaa agggtatctc
    20881 attaaaaaca gaaagcagtt gtcaggactg gcgctggaga tggacccgga gaataaattc
    20941 tccgggatga tgcagtaaaa attattggca atagtgcgat tgccggatgc aacgcttaat
    21001 aaggcgtaca tccggcaatg aaactcaagc taaaccaata aagaacccgg caatggtcgc
    21061 gctcatcagg ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct gggcgatttc
    21121 cggcgcgcgt tttggcgaaa tagccgaaaa cgctccaacg acaacaccga tagaaccaaa
    21181 gttagcaaac ccacagaggg cgaacgagat aatggcaatg gtcttgacat ccagcgtgcc
    21241 gctcgtttgc agatagggcg agaaattgag ataagcaaca aactcattaa tcgccaattt
    21301 ctgcccaatc aaacttccgg caagatttgc atcactccag tccaccccca taatccatgc
    21361 caacggggcc agcacatagc cgaaaatgct ttccagagag acattggcaa aaccaaacca
    21421 gccgccaact ccaccaataa tgccgttgat cagcgcaatg atcgccacaa atgccattac
    21481 caccgtcgcc acaccggcgg cgattttcag cccggtcatt gcaccgttcg ccgcagcttc
    21541 aataatgctt tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa cttgcgaagg
    21601 ttccgtcgcc ggactaagaa tacgtgcaaa caggataccg ccaggaatag ccatcagcga
    21661 agcggctaac aaatagtcaa tcggcacgcc cattccggca tagccaatca tcatcgaacc
    21721 cgcaatagac gccatgccgc tacaaatagc cgtaaatagc tcgttgcgat tcatgcgatc
    21781 aataaaaggt ttaacgatcg ccgggatctc attttgcccg aggaagatag tggtgactgc
    21841 gacaaaagat tcgattttgc tgatgttgag ggctttctga aaaatactgc caagaatacg
    21901 aatcagcagc cccattacgc caatgtagta cagcaagctg ataagcgcgg taacgaaaat
    21961 aatcgccggg agtacgcgaa aggcgaagat aaagcccgca ccgtcaaaca gaacatccat
    22021 tttgggaccg accagcgaac caaaaataaa ggcacttcct gcgtcactgt aggacatcac
    22081 tttatggacg cctaatgccg cctgctctac tgcccatttt cccgggggaa aatagagcat
    22141 gatgccacca atggcaattt gcagcagcag cgcagcccca accgtgcgca agctgatgct
    22201 ctttttattt accgacaaca caaaggcaat tgccagcaat accaccatcc ccacaacact
    22261 tctcattata tccataatga ttttcccttc atgccagtaa acccggcgtc agcgccaggt
    22321 tttggtatgt ttgatgagta cgggcgacgg ctttctgccc accagaaatt aacccacgag
    22381 acgctgatat tctttggcaa tttcgctcgc cagaatggcg ttattaaaga ccagttgaat
    22441 attggatttc aggctatcac cgccggttag ctccgcaaca cgcgccagca ggaacggtgt
    22501 actctctttc ccaataacac cctgttcttc ggcttcagca acggcttgat caattgctgc
    22561 attgatggtt tcttctggca tcgcaaactg ttccgggatc gggttcgcta ccaccaggcc
    22621 gccgttcaaa ccggtttgcc acttcactgc catcgcgcga gcaatttcgc gggcactgtc
    22681 gagacgaatg ctgacatcaa atgggctggt tcggcagaaa aacgcaggca gcgcttttgt
    22741 ctgataacca attaacggta caccgaaggt ttctaaatat tctgttgtta atcctaaatc
    22801 gagaatagat tttgcccccg cacaaacaac agtgacattg gtatttgcca gttcctgtaa
    22861 atcggcagaa atatcgaagg tatgttccgc tccgcgatgc acaccaccaa tacctccggt
    22921 tgcaaaaact ttaatcccag ccagtgctgc aataatcatg gttgatgcaa cggtagtcgc
    22981 gccatttttc cctgcagcaa cgacaaaagg taaatcacga cgactaactt tcgtcacgtt
    23041 atgtccttca cggcccagca attcgatttc ttctttactt aagcccactt tcatcacacc
    23101 gccaataatg gcgatagtgg ctggtactgc gccctgttta cgaattgttt cttctacttc
    23161 aattgcggtt tgggcatttt gtgggaatgg cattccgtga gaaattatgg tcgattccag
    23221 cgcaacaacc ggttttttat tttttaaagc atcctgtact tcagcggata tttgtaataa
    23281 ttcaggggac agagttaatt cagacattct gtgttctcca ctaacgatgt aacattggca
    23341 attgataatt cagggttgtt ggtatattca caggcaagtg ccattgacga acatccctgc
    23401 gcaaatcgaa cagaatcgat aaacggcata ccgtctaccc aacaggaggc cagccccgcc
    23461 atcatggcgt cgcctgcccc agtaacattg atgacattcg ttttaatagg ggccgaccac
    23521 ccactttcac cgttgatatc actgtagtaa acgccatccc ctcccatgct cagcaccagg
    23581 cggttcaggc cgtgttgatg aaaccaggcg gcgacctttg cgacatcttc acgtccggaa
    23641 agtgcaattc cgctcaacgt ttcggcttca agacggttag gttttaacgt atggattttg
    23701 ctcagatgat cgcggatttt gacgcatttc catgccgata ccggatcaat aaatacaggc
    23761 gtttccccgc tattttccag aacccagacc agcgcctctt cactaaggtt gcaatccgcg
    23821 acaataacct ttgcaccgcg aataaactcc tgatgctggg caagaaattc tgcagaaata
    23881 cagtcactaa tactcatatc atttatcgcg acaagcattt ctcctgtatt atcgagcagt
    23941 gataaatagc tggatgtatt ctcacccgga acaattaagc atttatcgac ataaacaccg
    24001 gattgattag tttgcgccag taatgattgc ccataaaaat caccacctac tgcacttaaa
    24061 agccaggcat ttttccccag taacgccaga ttatgagcga tattccttcc cacgccacct
    24121 ggggtaaatt taatttttcc gggattcgaa tccgcataat tcaatggatg atgtgaatac
    24181 ccagccacat caatattcgc cgagccaata attactatgt agtccttttc gcgcatagca
    24241 cgccccctgg caataaattg ccaacgaaga aattgggtaa aatagtcatt acagtcaata
    24301 agactattag agcatgtgtt cagatttaaa cacatgttca taataagcat gtttaacgta
    24361 atgtaaatcg atcagaaaaa acgaaatcgg ccagatatga aggcggtcac aaaatttgtc
    24421 tatttatgca gcaacaaaag agaaagttgt gatgtgtaac acacaatatg gcaggcaaga
    24481 aaaagggcag agatgtttct gccctcagat tgttgacaat gcgcgctttg ttcatgctgg
    24541 atgtggcgta aacgccttat ccgacctaca aaagcgcaaa aattcaatat attgcagggt
    24601 tcatgtaggt ctgataagca tagcgcatca ggcaattttg cgtttgtcat catcggggca
    24661 gagatgtctc atccccgatg atgaagagaa attatgcgtt tttttctact atttgtgttt
    24721 ccgggcgttt gaggaaggca taggccaaac ccgccaccag cgtaccggca ataattgcta
    24781 ccaggtaacc caataccggc gtaatagcgc cagggatcag cagaacaaac agaccaccgt
    24841 gcggagccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc gccccaccaa
    24901 cgatacagca cggtagcaca cgcatcggat cacgagcagc aaacggaatt gcaccttccg
    24961 aaataaagca cagaccaagt accagagcgg ctttgccacc ttcctgctgc gctttatcga
    25021 atttacgacg cgccaccatt gttgccagac ccattgccag cggtggcacc atacctgccg
    25081 ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca ccgaatgcgt
    25141 acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg aggatcgccc
    25201 ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcaac ccatcgagaa
    25261 tgccagcaac cggtttaccg attaggtaga tcatcgccag accgaccacc agactggaaa
    25321 ttagcggaat gatcaggatc ggtttcagtg cctccatact ctgtggcagt ttcagttgcg
    25381 tactgattaa cttcgcaatg taacccgcca ggaagcccgc aataataccg ccaatgaagc
    25441 cagaaccggt gctaaccgcc agcataccgc caatcagacc cggcgtgaga cccggacgat
    25501 cggcaatgga gaaggcaata taacctgcca gtaccggcac catcagcgca aaggctgaac
    25561 caccaccaat ctgcatcagc gccgctgcca acgtacccgg ctctttaaac gcttcgatac
    25621 caaaagcaaa agaaagcgcg atacacagac cacctgcaac aaccatcggc agcatgtaag
    25681 agacgcccgt cagcaagtga cggtaagcgc ctgcactctc tttcttacct tcagtggtcg
    25741 ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc agcaaccgct ttatccagtt
    25801 cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg atacatcggt ttaccagcaa
    25861 atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct gctgcgactt
    25921 cttcaggtgt gattgcatta cccgcgccaa cagaaccacg ggtttcaact ttcacccacc
    25981 agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg tgtgctacac
    26041 cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctagcagca accggtgctg
    26101 tcgcagcgac cggcgcagtg taaggtttcg catggccttt ggcttcactc aggaacagct
    26161 cagggtgcgc aactgcccgg gaaatatcgc ccagccagac atttttaccg ttcagcgcgc
    26221 tgtcattcgg gatgaaatca ccgagaacaa tcgccatttc tgcgtcgttc ggattgtcga
    26281 tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc
    26341 gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct ctcctgctgt
    26401 cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta
    26461 atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag acgcagtgtg
    26521 tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat agaatcccct
    26581 gcgccaacgg tgcttacgac atcgactgac ggtggtttgg cgatccattc gccggaggca
    26641 ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa catgcgcaat accttgttca
    26701 cgtagcgcat gtgcagcttc aatcacatct ttcatttcag gcagtttacg gcctgcccag
    26761 atttccagct cgcggcggtt aggtttcacc agccacggtg ccgctttcaa acctgctact
    26821 aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag gcgagtcatc
    26881 cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac acagaccata
    26941 tcgaactgac cgagccagct cagagaatca gtcacaaagc gttcccagtc ggcgggggta
    27001 acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt cagcttaacg
    27061 ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag ctcgctgaac
    27121 agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt gacatcaatt
    27181 cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg cagaccggtg
    27241 gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac aaggtcataa
    27301 gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc ccaagaccag
    27361 cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc tgggcggtaa
    27421 agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt
    27481 ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta aattgtttaa
    27541 tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg cgcaccacaa
    27601 actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc gccagcaacg
    27661 ttgccgcatc cgctttcagc aagcggtcag ctttattatc gagcaacaaa tcagcgagac
    27721 gcttaagaac cgcgatgggc tgatcgtcat tcattgccac actcaccagc atggctgccg
    27781 tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg ctacgcagat
    27841 tgccttcggc gctatcgctc agccagatac cctgtccgag gttcagcggt tgttcattga
    27901 tggctttggt gacgaaagtg gcgtcaactg cccccgcttc tttcagacgc gcagcgttca
    27961 gcgcctgaag agtcagcaga tcgctggcga cgatatccag cgtcagcatt tcgttgtcga
    28021 gcttcagctg ctcactctgc ttttcgccca tcagcaatgc gcgaagttct tctgctgttg
    28081 ttgctgactt cagttgttca gcaacggaat catcgctcag tacgtgggtc agctggcgta
    28141 gcaggcccag atgttcatcc gagctggcag caataccgat cgccacgtac gctacctggc
    28201 cgtcccccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt ttcagcacct
    28261 gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg aacgttgagg
    28321 tttgctgttc gcgcgccagc atgccattga cgtagccttc agctacatta ccggcctgca
    28381 ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt tcgcccggat
    28441 ggatgtcctg tacagataac tggaacatag ttctcctctc ttgctgaatt gaaacgattc
    28501 agcttctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa
    28561 acgtttcaag gaagcataat acttctgttt cagcgcgcaa ggaaagccgt aaagttatga
    28621 gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg atgggaaaat
    28681 ccgttatcag caatttcatt tcagcagtgt cagcagccaa tcagcaacag tgaaatggca
    28741 ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctgac aagcagcgta
    28801 aactccgcgt cttcctcttc cagtgatcga ccagcatgca taactccccc acagtcacca
    28861 gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat cgttgccttt ctcaccggta
    28921 ttgcgggcgc tctgcaaacc ccgacactca gtatttttct tactgatgaa gtacatgccc
    28981 gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt ctggtgagtc
    29041 agtttctcgc cgggcgctct gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct
    29101 gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac tttgttttgc
    29161 tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa atgtttgccc
    29221 ttgcccgtga acatgctgac aaaaccggac gtgaggcggt gatgttcagc tcttttttac
    29281 gcgctcaggt ttcactggca tgggtcattg gcccaccgct ggcttatgcc ttagcgatgg
    29341 gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt tgcggcgtga
    29401 tggtgtggct gtttttgccg tcgatgcaaa aagagcttcc gctggcgacc ggcacggttg
    29461 aagcgccgcg ccgtaaccgt cgcgatacgt tgctgctgtt tgtcatttgt acattgatgt
    29521 ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac gaactgcatc
    29581 ttcccgagaa actggccggt gtgatgatgg ggaccgccgc cgggctggaa atcccgacca
    29641 tgttgatcgc cggatatttt gccaaacgtc tgggtaagcg cttcttaatg cgcgttgctg
    29701 ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc gcattcacct gtcattctgt
    29761 tgggcttgca gctgctaaat gctattttta ttggcattct tggcggcatc gggatgctct
    29821 attttcagga tttgatgccc ggtcaggcag gttcagccac cacgctctat accaacactt
    29881 cgcgcgtggg ctggatcatc gcaggatcag tggcgggcat cgtcgccgag atctggaatt
    29941 atcacgctgt gttctggttt gcgatggtga tgattatcgc cactctgttt tgcttactgc
    30001 gaattaaaga tgtttaaggc gcggtcaaca tttccagatc gataagccag atcatggcct
    30061 gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag actttcggtc
    30121 gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg cagggagtat
    30181 tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg gcagtgcaac
    30241 acgccccgca acccggacgg cattccatac gttgttctct taaggttaac caccggattg
    30301 atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc acttgcccga
    30361 aaggcccgtc gcgagtactt tgtcgcgata tttttgacat ttttgactac aggaattttt
    30421 cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac ggcaaactgc
    30481 tgctggttaa ggacattgat attcagtcgc ccactgcccg cggtgccgct acgctgtaca
    30541 aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc aaaggtgatg
    30601 atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat gtcgatggca
    30661 acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc aaagatcaga
    30721 ttgaagaaga gttgctgttt atgcctgagg gcggcatgcc ggacatgcag gtgctgacct
    30781 gggacggtca gttgctggcg cttgagctgc cgcagaccgt tgatctggaa atcgttgaaa
    30841 ctgcaccagg tatcaaaggg gcatctgcca gcgcccgtaa caaaccggcg acattgagca
    30901 ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt cgtatccata
    30961 tcgaagaacg ccgttatatg ggtcgtgctg actaacttca gccgcatgca gaaaagggat
    31021 agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg cttcctgaac
    31081 accttacgcc cgcaattttc actcgttgat tgatcgccct cacacttcat cgcattaaca
    31141 atccagacca atttcaattg ctgtcatata actttacact gtcattgtta attaatcgtt
    31201 actaagacgt gactcctatg aatacaatcg cctccgttac gctcccgcat catgtacacg
    31261 ctccacgcta cgatcgacag cagttgcaat cacgtatcgt tcattttggc tttggagcct
    31321 ttcaccgcgc acatcaggca ttactgaccg atcgtgtgct gaatgcccag ggcggcgact
    31381 gggggatctg tgaaatcagc ttgttcagcg gcgatcaact gatgagccag ctccgcgcac
    31441 agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggcg ataattgtcg
    31501 gtgccgttca cgaatgtctg aatgcaaaac tggattcctt agcggcaatt attgagaaat
    31561 tttgcgagcc acaggtggca attgtttccc tgacaattac cgaaaaaggc tattgtattg
    31621 acccggccac cggtgcactc gacaccagca atccgcgtat tattcacgat ctacaaaacc
    31681 ctgaagaacc tcactccgcg ccaggtattc tcgtcgaagc actgaaacgc cgccgtgagc
    31741 gcggccatac accgtttacc gtgctctcct gcgacaatat tcccgacaat ggccatgtgg
    31801 tgaaaaacgc ggtgctggga atggcagaaa aacgttcgcc agaactcgcc gggtggataa
    31861 aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct gcaacaaacg
    31921 aatcactggc ggaaataagc cagcatctgg gggtgaatga tccctgcgcg attagctgcg
    31981 aaccgtttat ccagtgggtg gttgaagata acttcgtcgc tgggcgtcct gcctgggaag
    32041 tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa ctgcggatgc
    32101 ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc gcccatatca
    32161 gtgattgcat gcaggatcgc gcatttcgcc atgccgccag aacattaatg ctggatgagc
    32221 aagcgccgac actgcggatt aaagatgtcg atttaacaca atatgcggat aagttaattg
    32281 cacgttttgc taatccggca ctgaaacata agacctggca aatcgcgatg gatggcagcc
    32341 agaaattacc gcaacgcatg ttggcaggta ttcgcataca tctggggcgc gaaacggact
    32401 ggtcgttgct ggcattaggc gttgcaggct ggatgcgtta cgtcagcggc gttgatgatg
    32461 ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgtgaa cttgttgcag
    32521 gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaaatt ttcggtgatg
    32581 atctgccaga taacccgcat tttgtgcagg tcatcgaaca agcctggcaa caaatcgcac
    32641 aattcggcgc gcatcaggcg ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa
    32701 agcggatgaa gctcgccttc gtccgctctc cccttctctt ttctgccttt tttagccagg
    32761 attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccaga accaacctca
    32821 tcaccggttt tctcggcagc gggaaaacta cgtcgattct tcatctgtta gcccataaag
    32881 atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga attgatggtg
    32941 ctttgctcgc cgatagcggc gcactgctga aagagatccc cggcggttgc atgtgctgcg
    33001 ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga aaaccagacc
    33061 gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat ctcttaaccg
    33121 cgccagtcta tgaaccgtgg atagatctgc gcgccacgtt gtgcattctc gatccacgcc
    33181 tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg gctgccgcag
    33241 acattattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa gcgctagagc
    33301 gctggtggca gcaaaatggt ggcgatcggc aattaattca cagcgagcat ggaaaaattg
    33361 acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc
    33421 attctcatca gcatagcgtg aaaaaagggt tagcagcgtt aagcctgcca gagcatcaac
    33481 gctggcgtcg cagtctgaac agcggacaag ggcatcaggc ctgtggctgg atattcgacg
    33541 ctgatacggt attcgacacc attggtattc tggaatgggc gcgacttgca ccggtggaac
    33601 gcgtcaaagg cgtgctgcgt attcccgaag ggctggtgcg aatcaaccgt cagggcgatg
    33661 acctgcacat tgaaacgcaa aacgttgcgc caccggacag tcgtattgag ctgatttcca
    33721 gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt ttagcgacta
    33781 ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga ttaaaaattt
    33841 gccgcaaata gtgttgttga atattgtcgg cctcgcgctg tttctttcct ggtatattcc
    33901 cattaatcat ggattctggt tgccgattga tgcggatatt ttttatttct ttaatcagaa
    33961 actggtcgaa agtaaggcct ttttgtggct ggttgcattg accaacaatc gcgctttcga
    34021 cggttgttca ctgctggcga tgggaatgtt gatgctgagt ttctggctga aagaaaacgc
    34081 cccaggcaga cgacgtatcg tgattattgg tctggtcatg ctattaactg cagtggtatt
    34141 aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agtccaacgt tgacttttac
    34201 caatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct cacgagatag
    34261 ctttcccggc gatcacggta tgatgctgct tattttttcg gcattcatgt ggcgttattt
    34321 cggcaaagtt gctggcctta tcgcccttat tatttttgtg gtttttgcgt ttcccagagt
    34381 aatgattggc gcacactggt ttactgacat catcgtcggt tccatgaccg tgatattgat
    34441 cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct tttttgacaa
    34501 atcactacct ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa ttaacatcat
    34561 caggtttatt tttatagtga ggataatcct gatgatgcgc ctcctgcttt catccatcga
    34621 gcgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc aatcgtactt
    34681 taattgacca atcccgctga ttaactttct gtatcacttt ttcttataaa aaatcatgta
    34741 aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc ctcggcggta
    34801 gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct taaggaattg
    34861 tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata acgatatttg
    34921 tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga ttttgagata
    34981 tatcttgcgc gggatccccg cgattgcagt agcggttctg ctttctgcat gtagtgcaaa
    35041 taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa catcatcact
    35101 gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat
    35161 tatggatcag tatgctgact ggaaaggcgt acgctatcgt ctgggcggca gcactaaaaa
    35221 aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg gcttagaact
    35281 tccgcgttcg acttatgaac agcaggaaat gggtaaatct gtttcccgca gtaatttgcg
    35341 tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg gtatttatat
    35401 cggcaacaac cagtttgtcc atgcttccac cagcagtggt gttattattt ccagcatgaa
    35461 tgaaccatac tggaagaagc gttacaacga agcacgccgg gttctcagcc gcagctaata
    35521 aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca ctgcttaggc
    35581 agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg atccaggcta
    35641 tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta acggaatcat
    35701 gtttatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt
    35761 gatgattgcg atactggtga gctgccttca gtttttagtg gcctggcata agcacgaagt
    35821 caaatacgac acactgatta ccgacgtaca aaagtatctc gatacctatt ttgccgacct
    35881 taaatccact actgaccggc tccagccgct gaccttagat acctgccagc aggctaaccc
    35941 cgaactgacc gcccgcgcgg cgtttagcat gaatgttcgt acgtttgtgc tggtaaaaga
    36001 taaaaaaaca ttctgttcat ctgcaactgg tgagatggac attccactca atgaattgat
    36061 tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca cgccgatggt
    36121 gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa atagcggcgt
    36181 gtttgccgct ctgaatctca acctgacgcc ttcactcttt tatagttcac ggcaggaaga
    36241 ttacgatggc ctcgccctca ttattggtaa tactgcgcta tctacctttt cttcacgttt
    36301 gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg cgggcattcc
    36361 tctgaccgtt cggctttatg cagatgactg gacatggaac gatgtgtggt acgcattttt
    36421 actgggtggc atgagtggaa ctttcgttgg acttctctgc tattacctga tgagtgtgcg
    36481 tatgcgccca ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt acgtggtata
    36541 tcaaccggtg gttgatacac aagctttgcg ggtaacgggc ctggaagtac tgctacgctg
    36601 gcggcatcct gtagcaggag aaataccccc ggatgccttc attaactttg ccgaagcgca
    36661 aaagatgatt gtgccactga ctcagcacct gtttgagttg attgcccgcg atgccgcaga
    36721 attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc cggcccactt
    36781 gcacagcgaa agctttaaag cggatatcca gaaactgctc acttccctgc ccgcacacca
    36841 tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcgag aagccacaca
    36901 actcttcgcc tggctgcatt cggtcggcgt agaaatcgct attgatgact tcggcaccgg
    36961 gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgatcgtgg
    37021 atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg cggtgctgac
    37081 gctggcgaaa cgtctcaata tgctgacagt tgctgaaggg gtcgaaacgc cagaacaggc
    37141 acgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccgtt
    37201 accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt
    37261 gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa
    37321 aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc tgttcacctt
    37381 tggtgcgcag gcgcaggcta tcaaggaaag ctatgccttt gcagtactgg gcgaaccccg
    37441 gtacgcattt aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca
    37501 aataacgttg tctgccctcg gcaccttcga taatttcaac cgctatgcac tgcgcggcaa
    37561 tccgggcgca cgcaccgagc agctgtacga cacgctattt acgacttccg atgacgaacc
    37621 aggcagttat tacccgctga ttgctgaaag cgcacgctat gctgacgatt attcctgggt
    37681 ggaggtcgct attaatccac gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga
    37741 tgtagagttt acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta
    37801 caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt tagctaaacc
    37861 cggcaaagaa gatatgctga gtctgttttc gctgccggta tttccagaaa agtactggaa
    37921 agatcacaaa attagcgacc cgctcgccac gcctccgctt gccagtggtc cgtaccgcat
    37981 tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt actgggcagc
    38041 aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct
    38101 cgatgataat gtcgcctttg aagcgtttaa agcaggagcc tttgatttgc gtatggaaaa
    38161 cgacgccaaa aactgggcca cgcgctatac cggtaaaaat ttcgataaaa aatacatcat
    38221 caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt ttaatatcca
    38281 acgtccggta ttcagcgatc gccgggtgcg ggaagcgatc actctcgcct ttgactttga
    38341 atggatgaac aaggcgttgt tttacaatac ctggagtcgc acgaacagtt attttcagaa
    38401 taccgaatat gcggccagaa attaccccga cgccgcggaa ctggtgcttc tggcaccaat
    38461 gaaaaaagat ctaccgccag aagtcttcac gcaaatctac cagccgccgg tatccaaagg
    38521 cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aagcaggctg
    38581 ggtgctgaag ggtcagcaac gcgttaatgc cacaacaggt cagccactca gctttgaatt
    38641 attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg
    38701 gctgggtatc aacatggaca ttcgcaaggt ggataactcg caaatcacca accgcatgcg
    38761 cagtcgcgac tatgacatga tgccgcgcgt atggcgggcg atgccgtggc ccagttccga
    38821 tttacagatt tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca
    38881 aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa
    38941 attactgccg ttgggacgag cactggatcg cgtattaacg tggaattatt acatgctgcc
    39001 aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc aacccgctgt
    39061 acgcccagtt tacagcctgg gtattgatac ctggtggtat gacgttaata aagcgacgaa
    39121 actgccgtca gccagacaac agggagagta gatgggcgct tatctgatcc gccgtctgtt
    39181 gctggtgatc ccaacgctat gggcgattat cactatcaac tttttcatcg tgcaaattgc
    39241 gcctggcggt ccggtcgacc aggccatcgc cgccattgag tttggcaatg ccggagtatt
    39301 acccggcgca ggcggtgaag gtgttcgcgc cagccatgcg caaacgggcg tcggcaatat
    39361 cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg ccgagatcac
    39421 tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc tctgggacta
    39481 catccgtttt gattttggcg acagcctttt tcgcagcgcc tcggtgctga cgctgattaa
    39541 agacagtctg ccggtctcca tcaccctcgg attgtggagc acgctgatta tctatctggt
    39601 gtcgattccg ttaggcattc gcaaagctgt ttataatggg agccgttttg acgtctggag
    39661 tagtgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat
    39721 tgtcttcttc gcgggcggca gctatttcga tctgttccct ctgcgcggcc tggtttccgc
    39781 taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct
    39841 gccggtgctg gcgacagtga ttggcggctt tgcggctctg accatgctga caaaaaactc
    39901 attccttgat gaagtgcgta agcaatacgt ggtgacggca cgtgcgaaag gggtaagtga
    39961 aaaaaatatt ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt
    40021 tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg tgatgttttc
    40081 actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat
    40141 gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat
    40201 cagctatacg ctggtcgatc cgcgtataga ctttgaggga cgttaatgtc gcgactcagc
    40261 cccgtcaatc aggcccgttg ggcgcgtttt cgccataacc gtcgcggcta ctggtcgtta
    40321 tggattttcc tcctcttgtt tggtttgagt ttgtgttctg aacttatcgc caacgataaa
    40381 ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa
    40441 agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa
    40501 cgactggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc
    40561 aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg gctgggaacg
    40621 gatgccaatg gcggcgatgt gctggcgcgc attctctatg gcacgcggat ctcagttctg
    40681 tttggcctga tgctgaccct ctgctccagc gtgatgggtg tgctggcggg ggcgctacaa
    40741 ggctattacg gtggtaaagt cgatctctgg gggcaacgtt ttattgaagt atggtcgggg
    40801 atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa cttctggtgg
    40861 ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt gcgggcggag
    40921 tttttacgta cccgtaattt cgactacatc cgcgcggcgc aggcgcttgg cgtcagcgat
    40981 cgcagtatca tcctgcgtca tatgttgcca aatgccatgg tcgctaccct cactttttta
    41041 ccgtttattt tatgtagttc gataaccacc ctgacctcac tcgatttcct cggcttcggt
    41101 ctgccgctcg gttcaccgtc actcggcgaa ctcctgttac aagggaaaaa taaccttcag
    41161 gccccgtggc ttgggatcac cgccttctta tcggtggcga tattgttgtc tttgctgatc
    41221 tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca
    41281 aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa ctgttcgtac
    41341 cgtagtgaat gatgtgtcac tacacattga ggctggcgaa acgctggcgc tggtaggtga
    41401 gtctggttca ggtaagagcg ttaccgcgct gtcaatttta cgcctgctcc cttgcccgcc
    41461 ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc atgccagcga
    41521 tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg agccgatggt
    41581 gtcgttaaat ccattacata ccctggaaaa acagctttat gaagtgcttt cactccaccg
    41641 cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc gcgtcggtat
    41701 ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacgcca
    41761 gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc
    41821 aaccaccgcg ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca
    41881 aggcgagctg aatatgggta tgctgtttat tactcataac ctcagcattg ttagaaaact
    41941 ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagaaaaata acgccgctac
    42001 gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg aaccatctgg
    42061 cgacccggtg ccattgccag aacctgcctc aacgttgctg gatgttgaac aacttcaggt
    42121 tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa
    42181 aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg agtccggttc
    42241 cgggaaaagc acgacgggac tggcgctgct gcgactgatt aactctcatg gcagcatcgt
    42301 ctttgacggt cagccactgc aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg
    42361 cattcaggtg gtatttcagg atccaaactc ctcactcaac ccacgactca acgttttgca
    42421 aattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca
    42481 acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc
    42541 ggcggagttc tctggtggtc agcggcaacg tattgcgatt gccagggcgt taattcttaa
    42601 gccctcgctg atcattcttg atgaaccaac atcatcactc gacaaaacgg tgcaggcgca
    42661 aatattgacg ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag
    42721 ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc gacaagggga
    42781 agtagtggaa caaggaccgt gcgcgcgcgt gtttgccgca ccgcagcagg agtatacgcg
    42841 tcagctactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa
    42901 ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ttcgtcctgg cgatttacaa
    42961 acaggcacgg ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcttaacg
    43021 cctggcttag tttgtacatc accgaccatg cattatcacc atccgggcta agcaatttaa
    43081 gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac
    43141 ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt gagagttgta
    43201 atgaagtcac ttattttccc cggaagtaca ttttacttca caatttattt acatcaattt
    43261 taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgcctgcgt
    43321 ggcctatatg gctctgattt aaataatatc tgcgggcgtg gtcacgtttg cggggctata
    43381 tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat
    43441 aaattgattt tacataaaat aaacatatat cgggggaatg atagatttgt ggttgacatg
    43501 catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt tttttcggcc
    43561 gactggcgta cagacagaag agaatggagc tggttgcaca gaatgcaatt gaccaaatca
    43621 tcggccatgc agagttaaag gtcgcaagag aaagtaatgc gccaacaatt gcccctatgc
    43681 cgaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa
    43741 gaatgaccgc catcgcattg gatgacacca tcgacacgca gcccacaaac gccgcaacgc
    43801 caaccaccag cgaccagaat cccagcccca gcagtgcact gatgaccatc cacgctgcca
    43861 taataaactg tatccacaac cccgagcgga acatatttaa cgcgccaata cggcggacga
    43921 agcggctgtt aaagatggtc atcacgaaca gaaaaagaat gtttagcgca aagtaataac
    43981 caaagttttc cggcgcgacg tggttgattt caatgtagac aaacggtccg gcgcttaaga
    44041 atgagaacat cccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat
    44101 gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tggcgacgct
    44161 ccggtggtaa ggtttctttg atcagaaaga agatcattgc cgaagccaga atcgccgcca
    44221 cggcgaggat ccagaagatg taatgccagc tcagccacac cagcacccag ccgccaacta
    44281 tcggtgccat cagcggtgca atggtcgtca ccagcatgac aaacgacatc atccgtgaga
    44341 actcttcttt cgggtagata tcacgcatca aagcgttgat aaccacgctg gccgcagccg
    44401 cagccagccc gtggaagaaa cgcatcacaa tcagctgatc gatggtttgt gccaacgcac
    44461 acgccaccgc ggcggcggca aacaccagcg taccgccaag aaccaccggc ttacgcccga
    44521 agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac
    44581 tgagggtcat ctgcgtactg cccgccggta caccaaactg cgctgaaatt accggtagcg
    44641 caggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa
    44701 caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa
    44761 gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat
    44821 cagcatccag cgtaataccg ccaatacgtt cacgatgcag ctcaaccaca tggttaccca
    44881 cggcggcgaa catacgtttc acctgatgat aacgcccttc gctgatggtc aaacgaacct
    44941 gcgttggggt aatcacttcc agcaccgccg gcttagtgag atctttttcg ttatgcagct
    45001 gcacgccttt agcaaattgc tctgccgtat cgtcagctac gggtgattcc agcgtcacca
    45061 gataggtctt ctcgcaatga tggcgcgggg aagtaatgcg gtgcgaccac tgaccatcat
    45121 ctgtcattag taccagaccg gtggtatcga tatccagacg ccctgcagcg tgcagtttcc
    45181 acgctaccgg ttcatcaaga aaatagagca ccgttgggtg atccgggtcg tccgtggagc
    45241 aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg
    45301 ggttgccatc gtaagcgaca tcatgttcag ggagcagttt gaacgctgca ttacggacga
    45361 tttcgccatc gacggtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa
    45421 cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa
    45481 acggaggtcg ggtaatgacc cgaaaatctg aactgttgtc tgcccagtat agcggtctaa
    45541 taacttccct caagggaaaa agatccgtgg catactatta gcagaataat ctacctacgc
    45601 gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc cacgctcaac
    45661 cattttcgtc gtcataaaac ccctgccgta atcgtgctgc ccaccggtgc aggtaaaagc
    45721 ctggtgatag cggaactggc gcggctggcg cgtggtcgcg tgctggtgct ggcacacgtt
    45781 aaagaactgg tggcacaaaa ccatgcaaaa tatcaggcgc tggggctgga agccgatatt
    45841 tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg cagcgtgcag
    45901 tcggtcgccc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa
    45961 tgccaccgta ttggtgacga tgaagagagc caatatcagc aaatcctcac tcacctgacc
    46021 aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg actgggcaaa
    46081 ggctggatct accagttcca ttatcacggc atggtacgcg gcgatgagaa agcccttttc
    46141 cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacgccg
    46201 ccagaacggc tggatatgcc agtagtgcaa tacgatttca gccgcttgca ggcacagagt
    46261 aacggactgt tcagcgaagc cgatctcaac cgcgagctga aaaaacagca acgcattacg
    46321 ccacacatca tcagccaaat catggagttt gctgcaacgc gcaaaggggt gatgattttc
    46381 gccgcgacgg tcgaacacgc aaaagagata gtgggattac tgcctgccga agatgcagca
    46441 ctgattactg gcgacacacc cggctctgag cgcgatgtgt taattgatga ttttaaagcc
    46501 cagcgttttc gctatctggt caacgtcgcg gtactgacca ccggatttga cgccccgcac
    46561 gtcgatctta tcgccattct gcgccctacc gaatcggtga gtctttacca acaaattgtc
    46621 gggcgcggtc tgcgcctggc ccccggcaag actgattgct taattcttga ttatgcgggt
    46681 aaccctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt
    46741 ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc
    46801 gccgacggga cattgattga acactttggt cgtcgctgtc agggatggtt tgaagatgac
    46861 gacggacatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac
    46921 gctgaaaatg atattgccgc ccgccgttgc cgcgagtgtg acaccattct ggttgacccg
    46981 gatgatatgt taaaagcggc gctacgactg aaagacgcgc tggtattacg ctgtagcggc
    47041 atgtctttgc agcatgggca cgacgaaaaa ggcgaatggt tgaaaatcac ctattacgat
    47101 gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca gcgaactgcc
    47161 ttcgagcagc tttttatccg cccgcatacg cgcacaccgg gcatcccgct gcgctggatc
    47221 accgccgccg atgtcctcgc ccagcaagcc ttattgcgac acccggattt tgtcgtcgcc
    47281 cgcatgaaag gccagtactg gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt
    47341 cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag
    47401 tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc
    47461 ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa
    47521 agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat
    47581 ctacggtggc aaagaagcgc cgctggctgt cgaactggat cacgacaaag tcatgaacat
    47641 gcaagttaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat
    47701 caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc tgcacatcga
    47761 cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt
    47821 gtctctgcaa attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt
    47881 cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac gtccggcgct
    47941 gcaattgatc gcgcaagttc ggcggcgtgc ctttaatggt cagggtatcg gttgctggat
    48001 cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag ccaccgccgc
    48061 taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca
    48121 gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg cctgccagct
    48181 ctttcgacag cgattccagt tcaatctctt cacctgcctg tagctgctca ttacagtagc
    48241 tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga
    48301 aatcatcgac cgcttgcaac agcccacggt tttgcgcttt cgcgtttaac ccttcgctgg
    48361 caccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct ttcaggaaag
    48421 tgagatagcg ggtcgactct ggattggttt cccactcggt taaatcgata cgcgcaacaa
    48481 tatccgcatg attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacac
    48541 gcatactgct caggttgctc agaactgcca ccagcagata ctccaccgcc agatagcgat
    48601 agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca
    48661 ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc
    48721 gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc
    48781 tatagacccg atgcagttct gccaccatct caacgacggt ttccgtcggt tccagcaatg
    48841 aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa
    48901 tctggttgat atccagactc atgataaact ctcctttaag accgggcggt attcaaccac
    48961 cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcgg cgtaacgaga
    49021 atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt
    49081 catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg
    49141 aacaattgct cgcagagctg ctcaacatac tggaaaaaca taaggctccg accgaccttt
    49201 ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc
    49261 aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca
    49321 aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa
    49381 aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg
    49441 ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc
    49501 atttattcct acgtaagcat tatcggtcat ttcagcttcc tggtgttcgc cacctacttg
    49561 ctgatcctct tcccgctaac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc
    49621 gtcattctgg caacggcggg aatgacgcta ttactgatcg acagcgaagt ctttactcgt
    49681 ttccatctcc atcttaatcc catcgtctgg cagctggtta tcaacccaga cgaaaatgag
    49741 atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg
    49801 gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg gcgcttcgcg
    49861 cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt ttatatctgg
    49921 gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac
    49981 ccgatgacgg cacgacgttt tcttgagaag catggcctgc ttgatgcgca agagtatcaa
    50041 cgccgtctta ttgagcaagg caatccagac gccgtttccg ttcagtatcc gttaagcgaa
    50101 ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggcctg
    50161 aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt
    50221 tcgttcacgc gtcatatgag ctccggcaac actacagaca acggcatctt tggcctgttt
    50281 tatggcatct cgccaagcta tatggacggc attttgtcga cccgtacgcc tgcggcgtta
    50341 attactgcgc ttaatcagca aggctatcag ctggggttat tctcgtcaga tggctttacc
    50401 agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagtgt acgcacccaa
    50461 tccgacgaac agaccgccac gcagtggatt aactggctgg gccgctacgc acaagaagat
    50521 aaccgctggt tctcgtgggt ctctttcaat ggcacaaaca ttgacgacag caatcagcag
    50581 gcatttgcac ggaaatatag ccgggcagca ggcaatgtcg atgaccagat caaccgcgtg
    50641 ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg tggtgattat cactgccggg
    50701 cggggtattc cgctgagcga agaggaagaa acctttgact ggtcccacgg tcatctgcag
    50761 gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc gctgactgat
    50821 cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc
    50881 gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gccattactg ggttaccgcc
    50941 gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac
    51001 ggtaaatacc gcacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta
    51061 agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg attaattata
    51121 aatcagttag caaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca
    51181 cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt
    51241 tcaaatcctc tcgtgccgac caaaaatccc aagaaaacca acctattgcg gttggttttt
    51301 ttatatctgc atttaattcg ataaacagac cgcgacacat cacggcctgt ttattttctg
    51361 ttatcagaac gtccagacca cacccgcctg agttgtgctg ctttcttcac gggagaaacg
    51421 gctgtcgata ccaagcgtta gccccgtcaa tgtttgctca aagccagcgc ctgcatcagt
    51481 ggtcacgttg ttgcggctgt taattgccga actccacatc gccgtatcgt aattaacgcc
    51541 ttttacgcta ccaagacgcc cacgcacggt gtccagttct gcatcaaata ccagcggttg
    51601 tgctgccgcc atattcagca catccgtggt tgaagggggc atttgtgcgc gattctctgc
    51661 gagactccag ctatggttgc cattatccag caaggtatat tcatacgtac tgatatcaac
    51721 aacgcctccg gcattgccca acgtaaatgc cgcatcaccg cgcccgttgt taccagtgta
    51781 aggttatctc ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct
    51841 gttgctttaa cctccagtag ccctcccttt tctataacgg caaagacggc aatcccatct
    51901 ttttcaacaa ttaagcgccc tcctttaatc gtgggatcag tcgccgcgcc ataaacctgt
    51961 agcactccat tattgatcgt agtctggttt accgcgccac ccatttgaac aatttgataa
    52021 ccatcattta atactgagta ttctgcggtg ccatttattt caatgtactg atatcccccg
    52081 ttaatttcag tattactact tacaccaaat tcttatatat gctgttctcc gccaggattg
    52141 attttcgtat tattggcggc cctacaaacc agctggatat ctttttccag gacaacacca
    52201 tcaactgtct caccatactc aacagttgat gccaatgcta tgggcgaata taacgctgta
    52261 gccaccagaa cagatattgc ggaacgacaa agagaaacag aaccagattg gtgcattaag
    52321 ctttcatcct atgaaattaa ttgctgttaa aagcattggg gacagaaaat acccatagct
    52381 ccatacccgg agtcagtttt taaaaactgt ttaaagaaat gcacaagtat tgtgattgat
    52441 tttttagttg tttttcctga tgagaagctg atgcaaaatt ccgtctttat aataaagatg
    52501 atgccaaagc gaacgacaaa gttgtagttt tcactacatg tccatacata aaatggggta
    52561 acattcacgc gcctggtagc gttaccaacg ctacgctcaa acataatgat tctaataaaa
    52621 cctcaggaga ctactatgcc tgaagcaacg ccttttcagg tgatgattgt ggatgatcat
    52681 ccacttatgc gacgcggtgt tcgtcagtta ctggagcttg attctggctt tgaagtggtc
    52741 gccgaagcgg gcgacggcgc aagcgcgatc gatctggcga atagactgga tatcgacgtg
    52801 atcttgctgg atctcaatat gaaaggcatg agtggcctgg ataccctcaa tgccttgcgc
    52861 agggatggcg ttaccgcgca aattattatc ctgaccgtat cagatgcctc cagcgatgtc
    52921 tttgcactga tagacgcagg cgcagacggt tatctgttga aagacagcga cccggaagtg
    52981 ttactggaag cgattcgtgc cggagcgaaa ggcagcaaag tctttagcga acgcgtcaat
    53041 cagtacttac gtgaacgtga aatgtttggc gcggaagaag accccttcag cgtgctgaca
    53101 gaacgcgagc tggatgttct gcacgagctg gcacaggggc tgtcaaataa acagattgcc
    53161 tcggtgttga atatttccga gcagacagta aaagtccata ttcgcaatct gctgcgtaaa
    53221 ctcaacgtcc gctcacgcgt ggcggccacc attctgttcc tgcaacaacg cggggcacaa
    53281 taaaaatagc ccgatggcat caccatcggg ccacttttta tttactctcc tgcggcgaca
    53341 aatgttgcat cgcctgcgcg atactacgtt cgatcaccgc acggcgagta tcgttggcag
    53401 gtaagagttt caacatcatt tcccacgcgg caacggcttc gccaaatcgt tgctgctcaa
    53461 aggcattaaa tgcatacatg cttaacacac ggatattgct atggtccgtt ctcaccaact
    53521 ggcgcagcag ttcgccgccg aggcggttgt cgttgggatc agatgaacgt gtcaacgctt
    53581 cagcgtatcc cagtgcagca tcgctgtttt tcggatcgag gcgatacgca gtggcgtatg
    53641 catcggtggc gatactggcg ttacccagcg ccatgccaac gcggcccaac ataatccagc
    53701 cttctatatc tcccggattt ttttgcagtt gagtacgcat ccccagcgca aggcgcgaca
    53761 tctcttcttc gttgagcgga tcggctttcg gatccagcgc cctgtccagt aacgccggag
    53821 cctgtgccgt ggcctgctgc cagattttca cctgctgata attgccagtc tggtagtagc
    53881 tgacgccagc cacaattaac gccaccacaa tacccggcag ataaacaaca tatccggcac
    53941 gcttaccttc cggcacgctt tgttctggaa acgcttccgg caccacgcgt acccgacgcc
    54001 gcgaacgggc gtatatgacc caaccgccaa tgccaatagc cactactggc agcacccaca
    54061 gcagcacggt cagcggcgtt aacggcggat cgtaagtgac gaagttgccg taacgcgcca
    54121 ccatataatc gacaatctct ttcttacttt tgccttcctg catcagttca tacactttct
    54181 ggcgcaggtc ggtggcaatc atcgagttgg aatcggcaat gctgttgttc tggcatttcg
    54241 ggcagcgcag ttcttcagtg agctgacgga actgttgttc ctgtgcttca tctttaaact
    54301 gcaacacgtc gatggtcgcc agcgctgagc cggagatcat cagcatcagc acgcccaata
    54361 aaaacctcat tgtgcggcct ccttactgta tttctcccac agtggcttga tctcttcttc
    54421 ccagacgcga ggattgagat cgcccgcatg gcgatagcga atgatgccgt tgccgtcaat
    54481 aaggaacgtt tctggcgcgc catagacacc gagatccagc cctaacatgc cgtcgccatc
    54541 aaacaggctt agcgcgtaag gattgcccag ctctttcagc cagctgattg ccttctggcg
    54601 atcgtcttta tagttcatgc cgaccacgcg gatgccctgc gcagaaagct gattcagata
    54661 ttgatgttcc gcacggcagg tcggacacca ggtcgcccag acgttaagca gcactggttt
    54721 gccctgagtc agcacatccg cctgataaaa ctgccccgga ttgtccagtg attcgaggcg
    54781 aaacttcggc actggcttgc caatgagcgc cgattccaga ttggtcggat catccccttc
    54841 ggcattacgc gccagctgcc acagcagcgc cgcggcaatc gccaggaaga taatcaacgg
    54901 aattaacaat accttgcgct tcatgcagcc tccggcgcag ttttctgcgg gttcacgcgc
    54961 ttacgatagc gaggatcaaa cagacacagc agtccgccca acgccatcat cagcccgccc
    55021 gcccaaatcc agcgaacaaa tggtttgtag taaagacgca cggcccacgc gccgttttcc
    55081 agctcttcac cgagcgccgc gtacaggtca cgcgtgatgc caccgtcaat cgccgcttcg
    55141 gtcatcatcg acccggcagt gttgtaataa cgtttttctg catacagcac tgtttccggc
    55201 ttgccatctc gcgttacgcc gatagtcgcc acaccgccac gccagtttgg accagtcact
    55261 tctttgacat cacggaaggt gaagcgatat tcatgaatat cgacgctatc gccggacttc
    55321 atgcgcacat cacgctcaac gctatagttc tgactaaaag caatgccaac aattgtcact
    55381 gccagcccca ggtgagctgc caccatcccc caataactga aggtggtttt cgtgccgcgt
    55441 gaaatacgca gcgcagcttc cgcaattgcc agcaccgcaa tccagcaggc cattgccagg
    55501 ccgagcaccg tcatcgccac aactttgctt tcgaacagcc acggcaacag cagcgacagc
    55561 accagcgtag agatgaaggc gataatcaac aaattgcgga ttttacgtgg gcgatcgcgt
    55621 ccccagcgca ccagagggcc gacaccaagc agtagcgcaa acggcaccat cagccaggta
    55681 aacatggtgt tgaagaacgg ttcgccaatc gaaatactgc ccagtcccag ttgcttgtgc
    55741 accagcggca gcaacgtccc cagcaacacc accagcatcg cggctaccag caaaacgttg
    55801 ttcgctaaca gcaaagattc ccgcgaccac agcgcattgt ttacgcgtga gcgaactttg
    55861 tgtccacgcg cggcaaacag cagcagcgaa ccgccaatca ccagcaccat aaaggcgagg
    55921 ataaacatac cgcgcgccgg atcagacgca aacgcgtgta ccgataccag cacgccggaa
    55981 cgcacgagga aagtccccag cagacacaac gagaaggcac tgattgccag cagtaatgtc
    56041 cacgctttga agctggcgcg ttgttcagtg accgccagtg agtgcatcag cgcagtcccc
    56101 accagccacg gcataaacga ggcgttttct accggatccc agaaccacca gccgccccag
    56161 ccgagttcgt aataggccca tgcggaaccg agcacaatgc cgagcgtcag gaagatccac
    56221 gcagccagcg tccacggacg agtaaaacgc gcataagtgc tgtccagacg cccactcaac
    56281 aaagatgcaa tggcaaaagc aaacgccacc gagaaaccga cgtaccccat atagagcaga
    56341 ggcgggtgga aaatcagccc cggatcctgc aacaacgggt taagatcgcg cccttcaatc
    56401 gggaaattcg gcagcgtgcg tgagaacggg ttagaggtaa agagaatgaa cagcaaaaag
    56461 ccgacactga ccatccccat tatcgccagt acgcgggcaa caatatccag cggaatacgc
    56521 tgactaaaaa tcgctaccgc aaaggtccag ccgctcatca gtagcaccca cagcaggagc
    56581 gagccttcat gcgcgcccca ggtagccgcc acgcgatacc acaccggaag ctgggtattg
    56641 gagttgctgg caacataggt gacggtaaag tcgttgacca cgaaagcatt gaccagcacc
    56701 agaaatgcgc cagccacaga cataaacagc agccaggcaa acaagcggga agacgccatc
    56761 atgcgcgcgt ccccgcgcgc cacgccccat agcggataca cagacagcag cagcgcaatt
    56821 cctagcgcca gacacagcag cccgttacca atttctggca tcatgatgct gggtccttat
    56881 aaacactcgc cgggcgacgg tggttagctt ccatcgcttt ctcaacttct ggcggcgtgt
    56941 agttttcatc gtgtttcgcc agcacttctt tcgcgaggat atgattgcct ttttccagtt
    57001 cgccctgcac cacaacgccc tgcccttcac ggaacagatc cggcaaaatg ccttcgtaag
    57061 agacatctac tgaaccttca gcgtcgtaaa tggtgaaagt tacttttagc gaattgggat
    57121 cgcgctgcac actccccggc atcaccatcc cgccaacgcg cagacgctga ccgacttccg
    57181 gcatctgctg agtttcacgc ttgccgtaga gaatctcccc cggcgtataa aagagatcga
    57241 tattcgagcg cagcgcatat agcaccagac cgatagtcag cgccagcccc gccagcacgg
    57301 cacaggcaat ccacaagcgg tttttacggc gaatattcat gcagcctcct gctgttgcgc
    57361 agcacgtaaa cgcgcctcac gcgcccgctg ttgcgccacg ccacgcagaa ttgcgcgatg
    57421 ttgcatcacc gagtgcacga ctaaaaccac cagcggaata acggtcatca ccaccgccag
    57481 ccagacaaaa aaggcgtaac cgcccattgc gaaaaattca ttccaggaag caaatgcagg
    57541 ggtcatttac ggcctctttt cagtatcagt tcactcaccc acggacggcg tttttccatc
    57601 agcaaaatca aattacgcat ccgcatcagc gtcagcgtgg cagacaggag caggaagccc
    57661 aaaatcgacc agcgcagcgg cgaacgcatc gccggatcga tactttgctg cattcgcgtt
    57721 gatccctgat gcagggtgtt ccaccactct acggagtaat gaataatcgg cagattcacc
    57781 acgccaatca gcaccaggat acctgccgca cgcccagcca gacgacggtc gtcgaaggcg
    57841 tgccacaggg caatgacacc aacatacaga aacagcagca ccagttcaga agtcagacgt
    57901 gcatcccata cccaccaggt gccccacatc ggttttcccc atgcagagcc ggtaaccagg
    57961 gcaataaagg taaacacggc accaatgggg gccatcgccg ccaccgccag gttggccatt
    58021 ttcatctgcc agacaaggcc aataaacgcc gccaccgcca ttgatgcata aatgcccatc
    58081 gaccagatcg ccgcaggcac atgcaggtag ataatgcggt agctatttcc ctgctgataa
    58141 tcagccggag caaagccgaa cccccagatc cagccgacgg taagcacgac cacactggca
    58201 attgccagcc acggtataaa ccagccacag atttgataca gccgtggtgg gatcgccagt
    58261 tgatgcagtg ttttccacat agtttcgata ccagactcga acaaaaatca gtaatccagc
    58321 gttattgaat gctgattcgt aacgctgccg ccgtcgcaaa aggacttaat gtcgcggtgc
    58381 ctgccagcaa cgcgcctaaa attgccagat acccatcaac gggcaaatgc atagaagccg
    58441 cgtccatcgc ggcggtggca aagatgagta atgggatagt cagcggtaac accagtatgc
    58501 tgagcagcac accaccgcgc ttaagtccca ctgtcagcgc cacgcccggc gcaccaagaa
    58561 agccaagcgt aggggttccc agcagtagcg ttaacgccat cacttgccag ccataaacat
    58621 ccattcccag tagcattgct accaggggcg aaaggatgag taacggcaga ccggttacca
    58681 tccagtgcgc catcaccttc gccagcacaa cggcgggcaa gggtaacggc aacaacatca
    58741 attgttcaag actgccgtcc tgcaaatcgt cacggaacag tcgctcgagt gccagcaagg
    58801 atgaaagcag cgcggcaacc cagataatac ccggcgcaat acgcgccagc agttgcggct
    58861 ccggaccgat actgagcgga aaaagggtaa tgacaatcag gaagaaccac agcgggttgg
    58921 cgatttcggc gctgtggcga aacgctacgc gcagctcaag acggaaaatg cgccagaaca
    58981 tcatgcggcc ctcgtttgcg tcagtgaaat gcggcgaatt ttactttcag caacgttgag
    59041 cggctggtgg gtagtcagaa tcacaatccc cccctgctcc gtatgctgcg ccatacgctg
    59101 ggtcagacga tcgacgccat taacgtcaat cgcggtaaaa ggctcgtcga ggatccataa
    59161 cgtggcacgg gtcagccaca gacgcgctaa agcgacgcgg cgttgttgcc cggcagaaag
    59221 ttgatttacc ggaatatctt cgaatccggc aagcccggcc tgcgccaggg cttccagaca
    59281 ttgtgcggta tcgccatcgc gatgataaaa gtgcagattt tctaacgccg tcagccgggt
    59341 tttgatcccc ggctgatggc ctatccataa caggttttga tggtagctgt cgcgtacctg
    59401 atgcaagggc tgcccttgcc agagaacctc gcctgcatca gggcgagaca accccgtcag
    59461 caaacggaga agcgttgtct tccccgcgcc gttgctaccg gtgatttgta cccactctcc
    59521 tgcgttcagc gtaaatgata aaccactaaa taaggttcgt tcatcccgct cgcaaagtaa
    59581 ctctctggct tcaagcatac tcacgcaata accctgttaa aaacctggct cgacttcacg
    59641 catgtcgggc agcttgtgcg ctatcccttt atggcaatca atacaggttt gcccatcttt
    59701 caccgcctgg tcatgcatct tcgcggcaac cgatttctgg gcggttgtat ccatatactc
    59761 gaagttgtga cagttacggc actcctgcga gttgttgtcc ttcatgcgcc gccactcatt
    59821 ctgtgccatc gtcaggcgat gagcttcaaa tttctgcggc gtgtcaataa cgccaaaaat
    59881 tttaccatac agctctttac ttgctttgag cttgcgtatc atctttggca caaactcgtg
    59941 cggaacgtga caatccggac aggtcgcacg gacgccgcta cggttgttgt agtgcacgga
    60001 atccatgtat tcctgataca ccgtgttacg catttcgtgg cagctaatgc agaactcttc
    60061 ggtattggct ttttccatcc cggtgttaaa gccgccccag aagacgatgc caccgacaaa
    60121 accgatcaac agcagcgtcc ccagcgccag acggctgggg gtacgccacc atttccacag
    60181 gcgcttaatc agaccaggct tacggtcaga atttcccata ataacctctt atttcccgta
    60241 accttttgac ggggtaaagg tattccccac gattggcgcg gtatcggcct gcggtacgtg
    60301 acattgcaga cagaaataac gacgcggagc cacttccgcg cccactttgc cgtcgctgtc
    60361 cataaagtga gtaggactaa tgcgcggcgc gccagtggtg cgataacttt cgacaccgtg
    60421 gcactgcaag cagcggttgg tattggtcgt tacctgataa ccttcaacgc tatgcgggat
    60481 cattggcggc tgattcacat agttcagcgg catccgatcc tgctcttttg gcatccgaat
    60541 ggccccttcc tgcgtcccgg atacttccgg cgactggcta aaatcgacgc cattagccgc
    60601 ccaaaccgcg ccgcttacca ccagggccag catcgccgtc cattgacaca gcgctttctt
    60661 caggtcatgg cttttcatga tttcgctccc gaactccatc gtgtagttat tgtaaataca
    60721 tcctcagaac agacatccac gcagcgaccg cacgtcatgc aatcgcggct ggtgacctgc
    60781 accgggcttt gttcatccag caccggggca cgtagcacat gcggttccgg gcaaacatga
    60841 aaacaatcca tacagcggtt acatttctgc cgatcggtgg ccgcaacggt aatcacacct
    60901 ttgctaccca gcacgccata cagtgcacca accggacaaa tgtgcccgca ccagccgtgt
    60961 tcaacgacca gtagatcaaa taaaaacagc gcgagaataa gcagcgcgcc gctgccgaag
    61021 cccattacca ggctacgccc catcagggaa acggggttga tccattccca gatgagcgtg
    61081 ccagttaagg ccgatcccac cagaatgatc accaacagca cgtagcgaat gtggcgaggg
    61141 atcgtcgcag actgattgag gtcaaatcgt ctgcgtaacc aatttgctaa gtcggtaatc
    61201 gggttcagcg ggcagaccca gctgcaaaat aatcgctttc ctgccagggc gtagagcacg
    61261 gtgataatca ccgcaccagt cagcgctacc gtggcaggca gatgaccgct ggcaagactt
    61321 tgcagtgtca tcagcggatc ggtcagtgga acggtgtcga ataacaggct actgctgtag
    61381 ttgccgtgca agatccacac accaaaccac ggaccgctca aaaacatccc cagcacgaag
    61441 aactgacaaa ggcgacgtaa cactaaccaa cggtgactgc gccaccagcc ttttttctcc
    61501 agcgcctcgc gcccggcgtc acgtttacga tttgccattg ttcccctcca gccagccgaa
    61561 gcggtaatgg tgacctaact cccctttcgc cagtgacagc ggtaacacct tgattgccgg
    61621 ttgttccagc acgcacactt tttcgcactt accgcagccg gtacaggcgt cgctgtgaac
    61681 cgtcggcaga aagcgggcgt gcttaccggt tcgcgtgttg cgctccagct ccagggtgat
    61741 ggcctcatca attttcgggc attcgcgata acaaacatcg cagcgcagcc cctgaaagtt
    61801 gagacagttt tcctggtcca ccagtaccgc cagccccatc cgcgcgtcgt cgatcgattc
    61861 gatctcacga tccagcgcac cgctcgggca cactttggcg cacggaatgt cctcacacat
    61921 ttcgcaagga atatcccgtg cgacaaaata tggcgtgccc gccgacagac cagaggccag
    61981 cgtcgccagt tttaaggtgt cgtaagggca agcctgaaca cactgaccgc aacgcacaca
    62041 ggcactggca aaggcgttct cgtttatggc tccgggcggg cgcaaccgca caccagatgc
    62101 gcgtgcggtt tgctgttgta accccagcgc cacacccacg gcagccagcc cgcctgctgt
    62161 gcgaacgaca tcgcgcagaa agcggcggcg accattttga ggtttcgctg accgggacat
    62221 aatggcttac accttctcca gtttgaccgc gcacttcttg aagtccgtct ctttcgagag
    62281 cggatcggtc gcatccagcg tcagtttgtt aaccagctgt gcggcgtcga agaacggcat
    62341 gtacaccagg ccctgcggcg gacggttacg accgcgcgtt tcgacaatcg agatcacttc
    62401 gccacggcga gaaaccactt tcactttgtc gccacggcgc agatcgcgcg ctttcgcatc
    62461 cagcgggtga ataaacagaa ccgcttccgg gaaggcgcgg tgcagttccg gtacacggcg
    62521 agtcatactg ccggtatgcc agtgctccag gacgcgtccg gtagagagcc acaggtcgta
    62581 ctcttcatcc ggcgcttccg ccgcaggttc gaacggcagc gcgaagatca ccgctttgcc
    62641 atccggttta ccgtagaact tgtagccttc gcccgctttc acgtacgggt cgttaccttc
    62701 gctgtaacgc cactgcgttt ctttaccgtt caccaccggc cagcgcagac cgcgtgcttt
    62761 gtggtaatca tcgaacggcg cgagatcgtg accgtgaccg cgaccaaacc atgcgtactc
    62821 ttcgaacaac cctttttgca gatagaagcc cagctcgcga gattcgtcgt tcagctgatc
    62881 ttccgccagt tcggataccg ggaatttgct cacttccgga gtggcataca gaacctcata
    62941 cagcgttttg ccacgcagtt ccggtttctt agccagcagc tcttccggcc acacttcttc
    63001 agttttgaag cgacgggaga actgaactaa ctgccagaga tccgattttg cttcacccgg
    63061 tgcctgtacc tgctgacgcc agaactgagt acggcgttcg gcattaccgt aagccccctc
    63121 tttctctacc cacattgcgg tcggcaggat caagtccgct gccagcgcac tgaccgtcgg
    63181 atatggatcg gaaacgatga taaagttgcg cggatcgcgc cagcccggca tacgctcttc
    63241 attaatgttc ggtccggcct gcatgttatt ggtacacatg gtccagtaaa cattcagttt
    63301 gccgtctttc agcgcacggt cttgtgccac cgcgtgcaga ccgattttcg ccggaatggt
    63361 gccactcggg atattccact tcttctcgca aatatcacga tgtttctcgt tagtcaccac
    63421 catgtccgca ggcagacggt gagcaaaggt gcccacttca cgcgcagtac cacacgcgga
    63481 aggctgcccg gtcagggaga acggaccgca acccggctgg gaaattttgc cggtcagcag
    63541 gtgcaggttg tagaccaggt tgttggccca cacaccacgg gtatgctggt tgaagcccat
    63601 cgtccagtag gagatgactt tcttgttcgg atcggcatac agctgcgcca gttgttctaa
    63661 ctggtctttc ggcacgccgg tcatttcggc ggttttttcc agcgtatatt cagcaacgaa
    63721 ggctttgtaa tcttcaaagc tcatcggttc ggaggcgtca gaacccggat tcttcgctgc
    63781 tttttccagc ggatgggtcg gacgtaaacc gtagccgatg tccgtcgccc ctttgcgcag
    63841 gttaacgtgt ttgctgaaga agtcctgatt tatcgcattg ttttgaatga tatagttggc
    63901 gatatagttc aggatcacca ggtcagattg cggcgtaaag atgatgccgt tatccgccag
    63961 ctcgaagcta cgatgctggt aggtagaaag caccgccacg gtaacgttct ggttagagag
    64021 acgacggtta gtgatgcgcg accagaggat cgggtgcatc tccgccatgt ttgcgcccca
    64081 cagcacaaac gcgtcagcct gctcgatgtc gtcatagcag cccatcggct catccatacc
    64141 aaaggtacgc ataaagccaa ctactgccga cgccatacag tgacgcgcgt tcgggtcgat
    64201 gttgttcgaa cggaagcccg ctttaaacag cttggacgcg gcataacctt cccagatagt
    64261 ccactgacca gaaccgaaca taccgatcga ttccggccct ttttctttca gggcggtttt
    64321 gaatttctct tccatcacat cgaaggcctg atcccaggtg attggggtaa attcgccttc
    64381 tttatcatat ttaccgtttt tcatacgcag cagcggctgc gtcaaacggt ctttaccgta
    64441 catgattttg ggcaggaaat agcccttaat gcagttcagg ccacggttaa ccggtgcgtc
    64501 cgggtcgccc tgacaagcca ccacacgccc ctgctgcgtt ccgaccagaa cgccgcaacc
    64561 cgtaccgcag aaacggcacg gcgctttatc ccatttaatg gcttcctgct gaccaacaac
    64621 ggcgcgggca acgcccggca cgctgagacc ggcagccgcc gcagcggccg caacggcgtt
    64681 agctttcata aagctacgac gactgagttt catggtgttt cctcaccttg ctcttcctgc
    64741 tggtgataaa ccagcgacac cgccagcacg ccctctacgt tgcgtactga ctcaatggtt
    64801 tggatcagcg tttcgctgtc ttctgcttcc accaccacaa tcaactgacc gctcggcgcg
    64861 tcgctgacag caacttcaca gccggtaaag gcgttcagtt gggtgctgat gtctgaaatt
    64921 cgttcgcttt tggcctgcac gaccaggctg caaacttgcc agttagtgtg catggagata
    64981 ctccgcagtt atggctgata ccggacagct ggctgcacat gctccacatc cgttgcaaag
    65041 ttggctatta agttgcggct ggtagatccc ggagagcgtc gggcgaaaga taattgccat
    65101 tggctcacag ctatcctgac agcggcggca ttcaactgac tgatacgcca ggcacgcgtc
    65161 cccaatggtg aactgcaaat cccaggccct ggtgtggcgc ggagaaaata gcgattcggg
    65221 gcaagcctgc gcgcaggcgt aacagaagct gcactcgtta tttttgaaat taacgctcgg
    65281 atagctaccc gcgccgcgtt gcagaatgtt gttttcacaa gcattaatac aggcgtcgca
    65341 gcgggtgcaa tgggtcagaa aatgagattc atcaccggac cagggcggac ggataccgtt
    65401 actggctttg cgccagcgac cagtgagtat gccccgacgg gatgcatcaa tcttcacatt
    65461 gaccttccat cattaacgcg ctctgaaaat tgagagcgac caaataaacc gcataattaa
    65521 taagccattt ttatagccgc taagatatta aaggatgtgt caaagatgca taccccgatc
    65581 ggggtaaaac ctgtagcagg atcaaaagag tggcgggaag cgtggcaaaa acgggctttt
    65641 gctcacattt caaatggtta taaacatatt tatatagcga ttaattcgcc agagatattt
    65701 ctgctggttt gtttt
//
LOCUS       DACADA010000006        64430 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.006, whole genome shotgun sequence.
ACCESSION   DACADA010000006 DACADA010000000
VERSION     DACADA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.006"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            10..597
                     /locus_tag="ISB17_000379"
     CDS             10..597
                     /locus_tag="ISB17_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAP1978359.1"
                     /translation="MSFVLLIGLLEIFALICGHMLSGALDAHLDHYDSITTGHISQAL
                     HYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLFVVPVSLLFTIIAVH
                     YTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGKLTDQFGQIHYLLLE
                     PEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            624..2285
                     /locus_tag="ISB17_000380"
     CDS             624..2285
                     /locus_tag="ISB17_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP1978360.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            2525..2584
                     /gene="ibsE"
                     /locus_tag="ISB17_000381"
     CDS             2525..2584
                     /gene="ibsE"
                     /locus_tag="ISB17_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAP1978361.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2704..4137)
                     /gene="hldE"
                     /locus_tag="ISB17_000382"
     CDS             complement(2704..4137)
                     /gene="hldE"
                     /locus_tag="ISB17_000382"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAP1978362.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(4185..7025)
                     /gene="glnE"
                     /locus_tag="ISB17_000383"
     CDS             complement(4185..7025)
                     /gene="glnE"
                     /locus_tag="ISB17_000383"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAP1978363.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     ALLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7048..8349)
                     /locus_tag="ISB17_000384"
     CDS             complement(7048..8349)
                     /locus_tag="ISB17_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAP1978364.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8591..9211
                     /locus_tag="ISB17_000385"
     CDS             8591..9211
                     /locus_tag="ISB17_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAP1978365.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINRLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9275..10513
                     /gene="cca"
                     /locus_tag="ISB17_000386"
     CDS             9275..10513
                     /gene="cca"
                     /locus_tag="ISB17_000386"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAP1978366.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVSLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDSLFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10593..11414)
                     /gene="bacA"
                     /locus_tag="ISB17_000387"
     CDS             complement(10593..11414)
                     /gene="bacA"
                     /locus_tag="ISB17_000387"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP1978367.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGENKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11505..11873)
                     /gene="folB"
                     /locus_tag="ISB17_000388"
     CDS             complement(11505..11873)
                     /gene="folB"
                     /locus_tag="ISB17_000388"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAP1978368.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11978..12595
                     /gene="plsY"
                     /locus_tag="ISB17_000389"
     CDS             11978..12595
                     /gene="plsY"
                     /locus_tag="ISB17_000389"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP1978369.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12608..13540)
                     /gene="ttdR"
                     /locus_tag="ISB17_000390"
                     /gene_synonym="dan"
     CDS             complement(12608..13540)
                     /gene="ttdR"
                     /locus_tag="ISB17_000390"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAP1978370.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13747..14658
                     /gene="ttdA"
                     /locus_tag="ISB17_000391"
     CDS             13747..14658
                     /gene="ttdA"
                     /locus_tag="ISB17_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAP1978371.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14655..15260
                     /gene="ttdB"
                     /locus_tag="ISB17_000392"
     CDS             14655..15260
                     /gene="ttdB"
                     /locus_tag="ISB17_000392"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAP1978372.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15308..16771
                     /locus_tag="ISB17_000393"
     CDS             15308..16771
                     /locus_tag="ISB17_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP1978373.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16814..17827)
                     /gene="tsaD"
                     /locus_tag="ISB17_000394"
     CDS             complement(16814..17827)
                     /gene="tsaD"
                     /locus_tag="ISB17_000394"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP1978374.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18065..18280
                     /gene="rpsU"
                     /locus_tag="ISB17_000395"
     CDS             18065..18280
                     /gene="rpsU"
                     /locus_tag="ISB17_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP1978375.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18391..20136
                     /gene="dnaG"
                     /locus_tag="ISB17_000396"
     CDS             18391..20136
                     /gene="dnaG"
                     /locus_tag="ISB17_000396"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP1978376.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20331..22172
                     /gene="rpoD"
                     /locus_tag="ISB17_000397"
     CDS             20331..22172
                     /gene="rpoD"
                     /locus_tag="ISB17_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP1978377.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22250..22756)
                     /gene="mug"
                     /locus_tag="ISB17_000398"
     CDS             complement(22250..22756)
                     /gene="mug"
                     /locus_tag="ISB17_000398"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAP1978378.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22881..22956
                     /locus_tag="ISB17_000399"
     tRNA            22881..22956
                     /locus_tag="ISB17_000399"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22914..22916,aa:Ile,seq:cat)
     gene            complement(23010..23774)
                     /gene="nfeF"
                     /locus_tag="ISB17_000400"
     CDS             complement(23010..23774)
                     /gene="nfeF"
                     /locus_tag="ISB17_000400"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAP1978379.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
     gene            24062..24685
                     /locus_tag="ISB17_000401"
     CDS             24062..24685
                     /locus_tag="ISB17_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP1978380.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLCVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24792..26312)
                     /locus_tag="ISB17_000402"
     CDS             complement(24792..26312)
                     /locus_tag="ISB17_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP1978381.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            26619..28109
                     /gene="ygjG"
                     /locus_tag="ISB17_000403"
     CDS             26619..28109
                     /gene="ygjG"
                     /locus_tag="ISB17_000403"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAP1978382.1"
                     /translation="MITTFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(28151..28483)
                     /locus_tag="ISB17_000404"
     CDS             complement(28151..28483)
                     /locus_tag="ISB17_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAP1978383.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            28702..29685
                     /gene="ebgR"
                     /locus_tag="ISB17_000405"
     CDS             28702..29685
                     /gene="ebgR"
                     /locus_tag="ISB17_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAP1978384.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29869..32961
                     /gene="ebgA"
                     /locus_tag="ISB17_000406"
     CDS             29869..32961
                     /gene="ebgA"
                     /locus_tag="ISB17_000406"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAP1978385.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
     gene            32958..33407
                     /locus_tag="ISB17_000407"
     CDS             32958..33407
                     /locus_tag="ISB17_000407"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAP1978386.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            33470..34903
                     /locus_tag="ISB17_000408"
     CDS             33470..34903
                     /locus_tag="ISB17_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1978387.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            35037..36107
                     /gene="ygjJ"
                     /locus_tag="ISB17_000409"
     CDS             35037..36107
                     /gene="ygjJ"
                     /locus_tag="ISB17_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAP1978388.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            36124..38475
                     /gene="ygjK"
                     /locus_tag="ISB17_000410"
     CDS             36124..38475
                     /gene="ygjK"
                     /locus_tag="ISB17_000410"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP1978389.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPESWSPLFNGEATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            38701..40719
                     /locus_tag="ISB17_000411"
     CDS             38701..40719
                     /locus_tag="ISB17_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAP1978390.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(40764..41180)
                     /gene="higA"
                     /locus_tag="ISB17_000412"
     CDS             complement(40764..41180)
                     /gene="higA"
                     /locus_tag="ISB17_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAP1978391.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41177..41491)
                     /locus_tag="ISB17_000413"
     CDS             complement(41177..41491)
                     /locus_tag="ISB17_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAP1978392.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(41776..42912)
                     /gene="rlmG"
                     /locus_tag="ISB17_000414"
     CDS             complement(41776..42912)
                     /gene="rlmG"
                     /locus_tag="ISB17_000414"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAP1978393.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            42997..43500
                     /locus_tag="ISB17_000415"
     CDS             42997..43500
                     /locus_tag="ISB17_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1978394.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            43577..44269
                     /locus_tag="ISB17_000416"
     CDS             43577..44269
                     /locus_tag="ISB17_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAP1978395.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            44348..45334
                     /locus_tag="ISB17_000417"
     CDS             44348..45334
                     /locus_tag="ISB17_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1978396.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            45617..46582
                     /gene="alx"
                     /locus_tag="ISB17_000418"
     CDS             45617..46582
                     /gene="alx"
                     /locus_tag="ISB17_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAP1978397.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            46906..48150
                     /gene="sstT"
                     /locus_tag="ISB17_000419"
     CDS             46906..48150
                     /gene="sstT"
                     /locus_tag="ISB17_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP1978398.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(48154..48705)
                     /locus_tag="ISB17_000420"
     CDS             complement(48154..48705)
                     /locus_tag="ISB17_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAP1978399.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(48788..50275)
                     /locus_tag="ISB17_000421"
     CDS             complement(48788..50275)
                     /locus_tag="ISB17_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP1978400.1"
                     /translation="MQYLKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(50290..51702)
                     /gene="uxaC"
                     /locus_tag="ISB17_000422"
     CDS             complement(50290..51702)
                     /gene="uxaC"
                     /locus_tag="ISB17_000422"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP1978401.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            52185..53483
                     /locus_tag="ISB17_000423"
     CDS             52185..53483
                     /locus_tag="ISB17_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1978402.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            53613..54389
                     /gene="exuR"
                     /locus_tag="ISB17_000424"
     CDS             53613..54389
                     /gene="exuR"
                     /locus_tag="ISB17_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAP1978403.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            54734..55396
                     /locus_tag="ISB17_000425"
     CDS             54734..55396
                     /locus_tag="ISB17_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1978404.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKFEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            55400..55783
                     /gene="mzrA"
                     /locus_tag="ISB17_000426"
     CDS             55400..55783
                     /gene="mzrA"
                     /locus_tag="ISB17_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAP1978405.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            55930..56298
                     /locus_tag="ISB17_000427"
     CDS             55930..56298
                     /locus_tag="ISB17_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAP1978406.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            56336..56641
                     /locus_tag="ISB17_000428"
     CDS             56336..56641
                     /locus_tag="ISB17_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP1978407.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            56644..57048
                     /locus_tag="ISB17_000429"
     CDS             56644..57048
                     /locus_tag="ISB17_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978408.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            57038..57337
                     /locus_tag="ISB17_000430"
     CDS             57038..57337
                     /locus_tag="ISB17_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978409.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            57434..57916
                     /locus_tag="ISB17_000431"
     CDS             57434..57916
                     /locus_tag="ISB17_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP1978410.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGMNSLMFMKNLTISGGFLLLAITGPGAFSIDRLLNKKW
                     "
     gene            57986..58972
                     /locus_tag="ISB17_000432"
     CDS             57986..58972
                     /locus_tag="ISB17_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP1978411.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            59255..59623
                     /locus_tag="ISB17_000433"
     CDS             59255..59623
                     /locus_tag="ISB17_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1978412.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(59657..60553)
                     /locus_tag="ISB17_000434"
     CDS             complement(59657..60553)
                     /locus_tag="ISB17_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978413.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            60658..61359
                     /locus_tag="ISB17_000435"
     CDS             60658..61359
                     /locus_tag="ISB17_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP1978414.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            61382..61546
                     /locus_tag="ISB17_000436"
     CDS             61382..61546
                     /locus_tag="ISB17_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978415.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(61607..62917)
                     /locus_tag="ISB17_000437"
     CDS             complement(61607..62917)
                     /locus_tag="ISB17_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAP1978416.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(62945..64276)
                     /locus_tag="ISB17_000438"
     CDS             complement(62945..64276)
                     /locus_tag="ISB17_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1978417.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLM
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT        15451 a        17172 c        16494 g        15313 t
ORIGIN      
        1 cttttcgcta tttcctttgt actcttgatc ggccttttag aaatttttgc acttatctgc
       61 ggtcacatgc tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca
      121 ggtcatatta gccaggcact tcattatctc aatattggaa gattgccagc tctcgtcgtc
      181 ctctgcttac tcgctggttt ttttggtctt attggcatcc tgttgcaaca tgcctgcgtt
      241 acgctctggc aatcaccact ccccaacctg ttcgtggttc ctgtcagttt actgtttaca
      301 attattgcag tgcattatac cggtaaagtc gttgcaccct ggattccacg cgaccatagt
      361 tcagctatta cagaagaaga atatgttggt agcatggcat taattaccgg ccatcaagcc
      421 acttcaggta acccctgcga aggaaaactc accgatcaat ttggccaaat acattatttg
      481 ctactggagc ctgaagaagg aaaaactttc actaaaggag acaaagtgtt aattatttgc
      541 cgactttctg caacgcgata tttggcggaa aataatccct ggccccagat attgtgattt
      601 attaatcaaa aggaaattta ataatggatg atatcgttaa ttctgtgccc tcctggatgt
      661 ttaccgcgat tatcgccgta tgcattctgt ttattattgg aattattttc gccaggctct
      721 atcgtcgcgc ttcggcagag caagcatttg ttcgtactgg tttaagtggg caaaaagtgg
      781 tgatgagcgg tggcgcaatc gtgatgccga tctttcatga aataattccc atcaatatga
      841 ataccctgaa gctggaagtc agccgctcaa ccattgatag cctgattacg aaagatcgta
      901 tgcgcgtcga tgtcgtggtt gctttctttg tgcgggtaaa accttcagta gaagggattg
      961 ccaccgccgc ccagacgctg gggcaacgca ccctgtcgcc tgaagactta cgtatgctgg
     1021 tggaagataa atttgtcgat gccctccgtg caacagcggc acaaatgacc atgcatgagt
     1081 tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa taccgtagct gaagatctgt
     1141 cgaaaaatgg cctggaactg gaaagcgttt cacttaccaa ctttaaccag acctcgaaag
     1201 aacatttcaa tccgaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaga
     1261 cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga tgtggaagtc gcagtgcgtg
     1321 agaaaaaccg tgatgcactt tcgcgcaagt tggagattga acaacaagaa gcgtttatga
     1381 cgcttgagca ggagcagcag gttaaaaccc gtaccgctga gcagaatgcg aaaattgcgg
     1441 cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg aattctggct gaacgacaga
     1501 ttcaggaaac ggaaatcgac cgcgaacagg ccgtccgctc aagaaaggtt gaggccgaac
     1561 gtgaagttcg cattaaagag atcgaacagc agcaggtcac cgaaatcgct aaccagacga
     1621 aatcgatcgc tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaacc
     1681 ttgcactcgc agaagcagta agcgcccaac aaaacgtaga aaccactcgc cagactgccg
     1741 aagccgatcg tgctaaacaa gtggcgttga tcgccgcagc gcaggatgca gaaaccaaag
     1801 cggttgaact gaccgtgcgg gcgaaagcag aaaaagaagc cgcagaaatg caggcggcgg
     1861 ctatcgttga gttagccgaa gctacacgta aaaagggtct ggcggaagca gaagcacaac
     1921 gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca aaccagcctt aaattcaaac
     1981 tggcattatt gcaggcgctg cctgcggtaa ttgagaaatc cgttgagcct atgaagtcta
     2041 tcgacggcat caaaattatt caggtcgatg gtctgaatcg tggcggcgct acgggtgatg
     2101 caaacacggg taatgtgggg ggcggaaacc tggcagaaca agcgttatca gccgcactct
     2161 cttaccgcac acaggcaccg ctgattgact ccttgctcaa tgaaattggc gtttcaggcg
     2221 gctcactggc ggcattgact tcatccttaa gctcaacaac tcccgtcgaa gaaaaagcag
     2281 agtaaaccct cttctatgaa taatccctgg cccaaaaagc cggggatttt ttatatctgc
     2341 gttccgctaa aaggtgcaaa tgatcaggct gttgcagcat tttgcgtgac tgctcgggga
     2401 aggcaaaact gcctctggga aagcgttgcg cggggtccgg cgctcatcaa caatcgggga
     2461 gtcagcaagg ggctgaaacg ggaaagcccc tcccgaggaa ggggccttat agaaggaaag
     2521 ggttatgatg aagttcgtca tcatactggt tgtgttgtta ctgttaagtt tcccgactta
     2581 ctaacaactc atcagagggg ggagaaatcc tcccttaccc ttgttccttt actttaggtt
     2641 gaaaaaacaa cagcgtcaat agacctgcct gctacgaagc gagatctgtg aaccgctttc
     2701 cagttagcct tttttatcca gttggatctt cttgatgatg ttggtcgtcg agcaaccgtc
     2761 ttcaaagttg agcaccagca cttcgccacc gttggcccag acttctttac tcccggcaat
     2821 ctcttctggt ttatagtcgc cgcctttcac cagcagatct ggcaagatcc cggcgatcaa
     2881 gcgctgcggt gtgtcctctt caaacgacac cacccagtcg accgcttcca gtgcgcccag
     2941 cacaatcata cgctgttcga gcgggttaac ggggcgggaa tcccctttca gccgtttggt
     3001 ggaggcatcg ctgttcacag cgacaatcaa acggtcaccc agtttacggg catttgccag
     3061 ataagagacg tgcccggcgt gcaggatgtc aaagacaccg ttggtcatca ccactttttc
     3121 accgcgttta cgcgctgccg ctacagcaag cttcagttcc tcttcggtca tcacaccaaa
     3181 gccggtatct gcacgtccac gtactgcgtt ttccagctcg atcggcgaaa ccgtggatgt
     3241 ccccagtttg ccgaccacca caccagccgc cgcattggca aagaagcagg cttcttccag
     3301 cgaattaccc gctgccagcg ttgccgccag gacgccaatc accgtgtcgc ccgcaccggt
     3361 aacgtcatac acttcctgcg cctgggttgg catatgcagc ggcgctttac ccggttgcag
     3421 cagcgacata ccctgttcgg aacgggtcac taacagagcc gagagttcgt aatcggcaat
     3481 cagtttcatg ccgcgctcaa caatctcttc ttcggtctta catttaccga caacagcttc
     3541 aaattccgag agattcggcg ttaacagcgt agcgccgcgg tagcgctcaa aatcggtacc
     3601 ttttggatca atcagcaccg gaacacccgc tttacgcgcc agttggatca tctgctgtac
     3661 gcttgccagc gcacctttgg cgtagtcaga aagcaccagc gcgccaatcg aactcagtgc
     3721 ctgattaatc cgctcgtgca gcggctgcgg atcaacccct tcgaaacctt cttcgaaatc
     3781 cagacggatc agctgttggt tacgggaaag tacccgtaat ttggtgatag tcggatgcgt
     3841 cggtacagaa acaaagtcgc atttaacgtt gacgtcggcc agagatttac tgagcgcgcg
     3901 cgccgcatcg tcaatgcccg tcaacccgac caggcgtgca ttagcaccga gagaagcgat
     3961 attcatcgcc acgttagccg cgccgcccgg acgttcttcg atggtattca ctttaaccac
     4021 gggtaccggc gcttccggcg agatacggct ggtgggaccg taccagtaac gatccagcat
     4081 cacatcacca accaccatca ctcctgcacg ttcaaactct ggcagcgtta ctttcattcc
     4141 tgtctcctga gagattcaaa atttgcgcgc gataatacca tacttcattc ttccaccagc
     4201 cacttctgcc agcttgcccg caccagttca cgctctgcgg tgaagcaatc ctccgacaca
     4261 tggcccggca attcctgtaa tgccagatga tgaagttcat cgcgcagcgt cgtgtaagca
     4321 cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt tttgcgccag tagttccaga
     4381 atgcgcacgt tgtctgacca gcgcgttaac ttcggttttt cgtgagcgta gcgcaacacc
     4441 agatattgag taataaattc gatatcggta attccgcctt catcggcttt gatatcaaag
     4501 cgatcgcgat gtttattgcc gagatgggca cgcattttct cgcgcatttc ccgcacttct
     4561 gtttgcaggg ttttgccttc gcgcggcagc gtcataatct cccggcgtac tgcgtcaaag
     4621 tgcgcggtga gctgcggatc gccgtacact acacgcgcac gcaccagcgc ctgatgctcc
     4681 cacgtccagg cctcgttttt ctgataatca gcaaatgctt ctgcggatgt caccagcatt
     4741 cccgccgccc cggacggacg cagtcgtgca tccacttcat acaaaatgcc ggaagaggtt
     4801 cgcgtactga acagatgcat aatgcgttgc gccagacgca gataaaactg tcgcccgtcg
     4861 atttcccgct cgccgtcagt catcgcatcc attgggcaat catggaggaa gatcaggtca
     4921 agatcggaac tgtagcctaa ctcccagcca cccagcttgc cgtagccgac caccgcaaaa
     4981 ccacgccctt cgcgttcgtt caggtgattt ggcttaccgt agcgggcaac catttgaacc
     5041 cacgcctgct gaacgacggc atcaatcatg gcttctgcca gccaggttaa gtgatcgctc
     5101 actttcatta ccggtagcgt accggcgata tccgctgccg caatgcgtaa cagctgcgcc
     5161 tgtttgaact gacgcagcgc ctcaagctgt tgttcttcgt catcttccgg cacgcgcagc
     5221 aaatactggc gcagttcatc acggtaggca tccgtcgccg ttggttggta gagggtattt
     5281 ggatcgagca attcatccag caataatgga taacgcgcta gctggctggc aatcatcggc
     5341 gatgcagcac acagagaaat caaatgctta agcgccgcag ggaattcact aagcagctca
     5401 agataagtgg tgcgagtaac aatccccacc agcaaggcgg taatgcgcga cagcgtgaca
     5461 gcagcgtctt cacgcgcaca gacatcactt agcagatgtg gcatcagatg gtcgagcacc
     5521 tgacgtcctc gcggcccgat ggtgcgctta tccagctctt tgcggaaatc agcaatcagc
     5581 attagcacct gtttgcgatc atcctcgcta agatgcgcca gcactggcgt ggtgtcatct
     5641 tcctgcaacg catcctgcca cagctcacgc cactgttccg acagcgactc ttcctgagtt
     5701 tcactttcgt cgtcgccaat caattcatta aacacccggc gcacattggc catatgtgcg
     5761 gtcagcaccc cggtcaattg cggccagtca gcaaagtcca tcgcccacgc cagccgcgca
     5821 cggttaagct catcagaagg aagcgtctgg gtttgttcgt cgttaatgct ttgcagcagg
     5881 ttttccagac gccgcaggaa cagatacgcc actcgcaatt gttcagcatc gttttcagaa
     5941 agcagatgca gcgcggcaat ggcgctgagc gttggcagta aagagcgcga ttgcagcgac
     6001 ggttcgcgcc cgccgcgaat gagctggaac acctggacga taaattcaat ttcgcgaatg
     6061 ccgcctgcgc caagtttgat gttgtcggtc aaaccgcgtc gacgcacttc acgggcaatc
     6121 attcctttca tgttgcgcag cgactgaatc acgctgaaat cgatgtaacg acggaaaaca
     6181 aacgggcgca gcaacgcacg caactcgtta gcatagacgc cgtcgctatc ccccataatc
     6241 cgcgccttga ccatcgcgta acgctcccag tcgcgcccct gctcctggta ataatcttcc
     6301 agcgccgcaa agctcagcac cagcgggcca ctttccccaa acggacgcag ccgcatatcc
     6361 acgcgataga cgaagccatc ctgcgttggt tgatccagta ctttaatcag ccgctgcccc
     6421 atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc cgccctgggt acaaccatgt
     6481 tccggccagg caaaaatcag atcgatatct gaggagaaat tcaattcccc gccgcccagc
     6541 ttacccatgc ctaaaatcag cagcggttgc gcttcgccct gcgcattgca cggcgttccc
     6601 cattcgcggc agcaggcgtc atacagccag tcacgcgccg caacaatcag cgtctccgcc
     6661 agatggctga gctgctgcaa tatgctctct tcagtaacca gtgccagcgt ttgcgcccag
     6721 gcgatgcgca ccataatgcg ccgccggaat agccgcagct cgcgcattaa cccagcctcg
     6781 tcactcacat tactgagcgc ctcctgcaac catgcagcgt aatgctgcca ttcgtcggcc
     6841 tgcggcggtt ggctttccag ttccgtcagc cactctggat gcgcaatcac gctgtcctgc
     6901 acaaaatcac tgaaagtaag tactgacttc gcctgtgcgc taagtgattc ctcggctaaa
     6961 ggctctggca gccgctcaac aacggtctgc cagtactgct gtaacggtga agagagcggc
     7021 ttcataagcg attttatcct tgcctgatta acgttttccg ctgtgcaacc agaacggttc
     7081 ctgattgttt gcctcattac ggaaatgttc aatttcaatg cgttgcccgg tcgcaatagc
     7141 gtgacgcagc ccctgccagt tctccagcca gtcttgcgcg acgacaggat cgtagtaacc
     7201 cgccagcaac agtattgagt caatatcacg cgtcaggcgt ggcaattggt cacggtagcg
     7261 atcgcctaac ggctggcaga aaacgctttt cagttcagcg gcatggcggg aaagatggat
     7321 atcggcaaag cgtttgaagg aatcgctcat tttactctgc gctttggcat ctaaaaacgg
     7381 ctgccaggct ttgcttacca gccattcggt caacgccagc ttcgccattg ccgtttcggt
     7441 agagtagacc gccgttacgg cagaaaccgc agaagcaatg gtcgcctcgc attgagtcag
     7501 cagatcacgt aagtgagtgc tcgctttacg cggcacaatg ccaccgaaca gcatcagtgt
     7561 atgacgaacc aggccaatgg ctgccagcac ctgctctttc gccgcatcgt tgccgcgtac
     7621 ccacagttct tcatgatact gccattgcgc taacgccagc tcgagcgccg cttccagccc
     7681 ctgttccaca tcagcttttg ccgcaacatg caaaatggtg gtcggtttga tttcacgcgc
     7741 cggattgccc tgcgccagat gatagccacg cgctgctttg ctcaggctgc cctggcgtaa
     7801 tccggtttgc gataccagtt ggttcgccag tttcagcacc gcacgcatgt cgccgctaag
     7861 cagttccagt tccagctcac agataggttc agcaaattca cccgctttca cctcaccctg
     7921 atcgagagcg atttcaattc ggctgccatc gacctctact agccattttt cgcgataaaa
     7981 atcggtgcta aacagcggct gcacgcggga agcgagatcg gtaggcaatt cgccgttcgg
     8041 ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt tcgctcaacg ccacgttata
     8101 ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca actttcatgg tcatctcata
     8161 gcgaccgttt tcgccacgaa tacgcaagcc catgtcgtgc ccacgcagcc aattatccgg
     8221 cgtttcgtag taaatattca gcaactgcac ggggtcatgg tgctcgccgc ccagcgtatt
     8281 gagatggtca cgcagcgcct caacggcact gtgattaaca ataaacttta attcgatttc
     8341 ctgagccatg gcctttactt actggttatg tcacataagt gacgatgaac ggcgaactta
     8401 atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa ttgccatgca acgggcaatt
     8461 tgacgggcgt aaaagtttga agcagtggca agacctgaca cagaggattc cgattgatgt
     8521 cgaatgcttt gcgtagcgac actgatgcca ctactatcgt tccactttcc atgacaataa
     8581 cgacagcctg atgccaaaat tacgcctgat cggattaact ttactcgcac ttagcgcgac
     8641 tgccgtctca cacgctgaag aaacgcgcta tgtttccgac gaactgaata cctgggtccg
     8701 tagcggtccg ggagatcatt atcgcctcgt gggcacggtt aacgccggcg aggaagtgac
     8761 cttattacaa actgacgcca acaccaatta tgcccaggtg aaagacagct ctggccgtac
     8821 tgcctggatc ccgttgaaac aacttagcac tgaaccaagc ctgcgctccc gtgtgccaga
     8881 tctggaaaat caggtcaaaa ctctgaccga taaactcacc aatatcgata acacctggaa
     8941 tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagt gacagcgtga tcaacaggtt
     9001 aaaagaagaa aatcagaaac tgaaaaacga gctgattgtc gcgcagaaaa aggtcgatgc
     9061 cgccagcgta cagttggatg acaaacagcg caccatcatc atgcagtggt ttatgtatgg
     9121 cggtggcgtg ctggggcttg gcttgctgct tggtctggta ctgccgcacc tgatcccaag
     9181 ccgcaaacgc aaagatcgct ggatgaacta aatcgccttc ttcgttgcac tgtcttatta
     9241 ttaggaattg tgtatttgac gaagggatga tggcgtgaag atttatctgg tcggtggtgc
     9301 tgttcgggat gcattgttag ggctaccggt caaagacaga gattgggtgg tggtcggcag
     9361 tacgccacag gagatgctcg acgcgggcta ccagcaggta ggccgcgatt ttcctgtgtt
     9421 tctgcatccg caaacgcatg aagagtatgc actggcacgt accgaacgga aatctggttc
     9481 cggttacacc ggttttacct gctatgccgc accggatgtc tcgctggaag atgatcttaa
     9541 gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat aacggcgaga ttatcgaccc
     9601 gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc catgtttccc ccgcttttgg
     9661 cgaagatccg ttacgcgtgt tgcgcgtagc gcgttttgct gcgcgttatg cccacctcag
     9721 tttccgtatt gccgatgaaa ctctggcgct gatgcgcgag atgacccatg cgggtgaact
     9781 ggaacacctg acgcctgaac gggtatggaa agagacggaa agcgccctca ccacccgcaa
     9841 tccccaggtg ttcttccagg tactgcgcga ttgcggcgca ctgcgcgttt tattcccgga
     9901 aattgactcc ctgtttggcg ttccggcccc tgccaggtgg cacccggaaa tcgacacggg
     9961 tattcatacc ttaatgacgc tctcaatggc ggcgatgctg agtccgcagg tcgatgttcg
    10021 tttcgcaacg ttatgccacg atctcggtaa aggactgacg ccgccagaac tttggccacg
    10081 tcatcatggt catggcccgg cgggtgtgaa gttagtggaa caactatgcc agcgtctgcg
    10141 cgtgccaaat gaaattcgcg atttagccag actggtagct gagtttcacg atctcatcca
    10201 cacctttcca atgctgaacc cgaaaaccat tgttaaattg tttgattcca ttgacgcctg
    10261 gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc gaggctgacg tgcgcggcag
    10321 aaccggtttt gaatcagcgg actacccgca aggccgctgg ttgcgcgaag cctgggaagt
    10381 ggcgcagtca gtgccgacaa aagccgtcgt tgaagcagga tttaaaggtg tggagattcg
    10441 tgaggagctg acccgacggc ggattgcggc ggtagccagc tggaaggaac aacgttgccc
    10501 aaagcctgat taacttttta aattcagtat attgcagaaa tcccttaggt cggataagac
    10561 gcacaagcgt catatccgac actatctctc aatcaaaaga acacgacgta caccgcagcc
    10621 gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg
    10681 aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc
    10741 gggatatcgc cggtagtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc
    10801 atcatcatcg gcaccgccag caggaacgag aactcggaag cagcgtaacg gctcaccccc
    10861 atcagcatcc cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga
    10921 cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca
    10981 cgcggctctt tcggcttcag gcattcggca gcaatcagca acagaccgcc aacgaccagt
    11041 gcatacatga catttatcgg gttaaacaat gatttaatcg tgtcgtggaa caacagcccc
    11101 agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgttt
    11161 tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc
    11221 cagaacatca ctactaccgc cagaattgat cctaactgga tcacgacttc aaaggttttc
    11281 gccgtgtcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat
    11341 accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag
    11401 tgcatatcgc tcatcaataa acccctaaat tataaaaatg taacgcttgt ccgaactact
    11461 gcagtatgac caggttataa tcgtttggtt taacagctgt aaaattaatt attttctttc
    11521 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
    11581 ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ttccgccacc
    11641 tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt
    11701 tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg
    11761 ttatcccacg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
    11821 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
    11881 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
    11941 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
    12001 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
    12061 tgggttaccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
    12121 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
    12181 gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
    12241 cgccgcctgt cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt
    12301 tgctaccgct tttggtgcca tcgcaccaat tggctgggat ctcactggag taatggcggg
    12361 aacctggtta ctgaccgtgc tattgagtgg ttactcgtcg ctgggagcga ttgtcagtgc
    12421 actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat
    12481 gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgcca
    12541 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
    12601 ggtggatcta catgacctga tagccttcgt cgggcttgcc cagccgttgc tggcaccatg
    12661 ccgccagaaa ttccacacag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
    12721 cagcccagat attggcgctt tgtgcatact ccggcaatac tcgcaccagt ttgccactct
    12781 ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc
    12841 attgcaacac aatctcgccg ctattggagg aaagatgtcc gcttaccttc accgattttt
    12901 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
    12961 ggcagtcatg acgacttaat tcctgtaagg attgtggctg cggatatttt tgcagatact
    13021 caggtgctgc gcataatatt cttttatttt ttgtcaaaag atgcgcaata taataatcag
    13081 gaattgcgtc attaatacga atatccagat caatattatc ctgcactaaa tcaatttgtc
    13141 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcatcagt tcggtaatag
    13201 ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatgcggatc atcccctccg
    13261 ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca
    13321 gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga gcggagcggt
    13381 tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag
    13441 gcgtttgccc aagcgtcgcc gctgcagcac taaaactccc gctatggacg atttccacca
    13501 gtacctgcaa gtctttggct aaaggccagc tatttagcat tgttaaatat ccgcagtttt
    13561 gataaacgca gtgtatttcg caaaacatta ccattccctg ctctggcgca atattcgttc
    13621 ttattttcgc gttgattcac gaactgttaa ttacatattc acggtggcaa aaaatataaa
    13681 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac ataaactgga
    13741 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac ggagattgtc
    13801 gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa
    13861 cagctaaagg atgccgaaac atcgtcgatg ggaaaaatca tctaccacac gatgttcgat
    13921 aacatgcaaa aagcgatcga cctgaatcgt cctgcctgtc aggacaccgg cgaaatcatg
    13981 ttttttgtta aggtcggttc ccgtttccca ctgcttggcg agctgcaaag catactcaaa
    14041 caagccgtgg aagaggcaac cgtcaaagcg ccactgcgtc acaatgcggt agaaattttt
    14101 gacgaagtaa acaccggcaa aaataccggc agcggcgtac cgtgggtcac ctgggatatc
    14161 gtccccgacg gtgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta
    14221 cctggccgat cgaaagtgtt aatgccgtca gaaggctacg aaggcgtagt gaaattcgtc
    14281 ttcgaaaata tctccaccct cgccgtaaac gcctgtccgc cggtactggt gggcgttggc
    14341 atcgctacct cggtggaaac cgccgcagtg ctctcgcgaa aagccatttt gcgcccgatt
    14401 ggcagccgcc accccaatcc gaaagcggca gagctggagc tacgcctgga agaaggactc
    14461 aaccgtttgg ggattggtcc acaagggctg actggcaaca gttcagtgat gggcgtgcat
    14521 atcgaatctg ccgctcgcca tccgtcaacc atcggcgttg ctgtttctac cggctgctgg
    14581 gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa tctgtctcac
    14641 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
    14701 atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt
    14761 gtcaccgtcg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
    14821 ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa atgggagatg gtatccgtcg
    14881 gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg
    14941 tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat
    15001 tcaaggcgct gcatgtgatt ttcccggctg gctgtgcggt actggcggca actcaagtgg
    15061 aagagattga agaagtgcac tggacagaac tcgggatgcc agagtcgctg tgggtctgcc
    15121 gggtcaaaga gtttggcccg ctgattgtct ctatagatac ccacggcaac aacctgatag
    15181 ccgaaaataa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc
    15241 acgtccacta catcaaataa ccctcacgga gaggctcgcc cctctccttt ttcgcaggca
    15301 taacacgatg aaaccttcca ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc
    15361 cattattgct ctacttccgc ttcccgcagg gctggagagt catacctggc tctactttgc
    15421 cgtttttact ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc
    15481 gatggtgggt atatccatca tcgccattct ttcgccgtgg ctgctgttta gcccggagca
    15541 gctcgcccag ccaggtttta agttcaccgc caaatccctc tcctgggcgg tttctggttt
    15601 ttctaattca gttatctggc ttattttcgc cgcctttatg tttggtacgg gctatgaaaa
    15661 aaccgggctt ggacgtcgca tcgcgctgat tctggtgaaa aaaatggggc atcgcacgct
    15721 gtttctcggc tatgcagtga tgttctccga gctgatactc gcgcctgtaa caccgtccaa
    15781 ctcggcgcgt ggtgcgggga ttatctatcc catcatccgt aacctgccac cgctctatca
    15841 atcacaacca aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat
    15901 tgttgccgac tgcgtgacca gcgccatttt cctgacggcg atggctccaa atttgctgtt
    15961 aattggactg atgaaaagcg catctcacgc catgctaagc tggggcgact ggttcctcgg
    16021 gatgttgccg ctcagcattt tactggtttt gctggttccc tggctggctt acgtgctgta
    16081 cccgccggta ctgaagtctg gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc
    16141 aatgggcccg ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcactggt
    16201 gttgtggatt tttggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc
    16261 gctgatgctg cttctgcgca ttatcagttg ggacgacatt gtcagtaata aagcggcgtg
    16321 gaacgttttc ttctggctgg cctcgcttat caccctcgct accggactca acaacaccgg
    16381 ttttattagc tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat
    16441 agtgatggtg gcgttgattg tggtctttta tctgctgcgc tacttttttg ccagcgccac
    16501 agcgtatacc tccgctctcg caccgatgat gattgccgcc gcgctggcga tgccggaaat
    16561 cccgctgccg gtgttctgcc tgatggttgg tgcggcaatt ggtctgggga gcattcttac
    16621 accatacgcc accggaccca gtccgattta ctacggcagt ggttatctgc caacggcgga
    16681 ttactggcga ctgggggcga tttttgggct gatattcctc gtattgctgg tgattaccgg
    16741 cttactgtgg atgcccgtgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg
    16801 gcacccaata gttttacgcg gccggtaact ctgccagtgg ccagcgcgga cgcacactca
    16861 cgccgagatc cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg
    16921 caccgttatc cgtacaaaat tccggacgcg cgtagaacac ttcgccacgg cgttttttca
    16981 tcatttccgc cagtttcgcc cgcaacgtgc ggttagcact cacgccgcct gccatgacca
    17041 gtcgcttaaa gcccgtctga tccagtgctc gcttacactt aatcatcaac gtatcgacca
    17101 ctgcatcttc aaaggcgcgg gcgatatcgg cacgcgtctg atcgtcagtg ccgttgtcac
    17161 gtatggtatt tgccgcgaag gttttcagac cagaaaagct gaaatccagc cccggacggt
    17221 cggtcatcgg acgcgggaaa acaaagcgcc cggcagtacc ctgagccgcc attttagaca
    17281 gtaacggtcc gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac
    17341 cagcagcatc atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga
    17401 ttaactgcgt atgaccgccg gaaaccagca gcgcgacaaa cggaaattcc ggtgggttat
    17461 cttccagcat cggcgctaac agatgtcctt ccatatgatg taccgggatt gccggaacgt
    17521 cccaggcaaa cgccagagaa cgccccacgg tcgcgccaac cagcagtgcg ccgactaatc
    17581 ctgggcctgc ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta
    17641 gcgcctcctg gatcaacggt acggttttac gcacgtgatc gcgggaggcc agttcaggca
    17701 cgacgccgcc gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac
    17761 ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta
    17821 cacgcatgac ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga
    17881 tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt
    17941 ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca
    18001 ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag
    18061 gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt
    18121 caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga
    18181 aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa
    18241 actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct
    18301 ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt
    18361 tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt
    18421 aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa
    18481 aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtcattc
    18541 accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg
    18601 atcgacttcc tgatgaacta cgacaagctc gagttcgtcg aaacggtcga agagctggca
    18661 gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc
    18721 catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct
    18781 ttacaacaac ctgttgccat gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac
    18841 gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa cgtcctgaag
    18901 cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcgggcat gttggtcact
    18961 aacgatcagg ggcgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgtgat
    19021 aaacgcggtc gggtgattgg ttttggcgga cgcgtgctgg gcaacgatac ccccaaatac
    19081 ctgaactcgc cggaaacgga cattttccat aaaggccgcc agctttacgg tctttatgaa
    19141 gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg
    19201 gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc
    19261 gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc
    19321 gaccgtgcag gccgcgatgc cgcctggcga gcgctggaaa cggcgttgcc ttacatgaca
    19381 gacggtcgtc agctacgctt tatgtttttg ccggatggcg aagaccctga cacgctggta
    19441 cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc actctccgca
    19501 tttctgttta acagtctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt
    19561 ttgagtacgc tggcactgcc actgatatcg caagtgccgg gcgaaacgct gcgaatatat
    19621 cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta
    19681 atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc
    19741 atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg
    19801 cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac
    19861 acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca
    19921 aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt
    19981 gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc
    20041 caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag
    20101 ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag tgccgaagag
    20161 cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct
    20221 cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc
    20281 gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa
    20341 acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct
    20401 atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca
    20461 tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg
    20521 atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc
    20581 tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg
    20641 aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg
    20701 aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg atcacctatc
    20761 tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg
    20821 gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg
    20881 agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggtgacgacg
    20941 ataccgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac
    21001 tgcgcgctca gtacattgta acgcgtgaca ccatcaaagc gaaaggccgc agtcacgctg
    21061 ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga
    21121 agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag
    21181 aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta
    21241 ccctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca
    21301 agccgtggtc ggaaaaactg catgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac
    21361 tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc
    21421 gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact
    21481 tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc
    21541 tgattcagga aggtaacatc ggcctgatga aagcggttga taaattcgaa taccgccgtg
    21601 gttacaagtt ctccacctac gctacctggt ggatccgtca ggcgatcacc cgctctatcg
    21661 cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaagctca
    21721 accgtatttc tcgccagatg ctgcaagaga tgggccgtga accaacgccg gaagaactgg
    21781 ctgaacgcat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc
    21841 caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg
    21901 aggataccac cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa
    21961 cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg
    22021 gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc gacgttaccc
    22081 gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt
    22141 ctgaagtgct gcgtagcttc ctggacgatt aatcggtagg ccggatcagg tgttacgccg
    22201 cacccggcac tagtccctcc gcacaaacgc caccttttcg gtggcgtttt tatcgcccac
    22261 gcaccaccag cgcctggtcc agctcgcgat acgcttcaac cagtttctcc agtgaaacgc
    22321 gacttaaacc gctgggattt ggcagcaccc aaatctgcgt cgaaccaatg gtgagcgttt
    22381 gtttccccca ctgtgcaccg cgctggctga atccctgttc atatgcttgt ttgcccagaa
    22441 tcgccaacgc ctgcggctga taatcttcaa ttttttcaat cagcttacgc ccgcctgcgt
    22501 gtagctcctg ctttgaaact tcattggctt gcaccgttgg acggtctacc agtttggtga
    22561 cgccacaacg ataatccagc agatgctgtg cctcctgcgg cttcaactga cggtcggtaa
    22621 acccggcctg atatatcacc ttccagaagc gatttgccgg atgagcaaag ggaaaaccag
    22681 tcccggcgga tgaaagacca gggttgatac cgcaaaacac gacccgtaac cctggagcca
    22741 aaatatcctc aaccatattt actcctttct aacatcacca ggaaaagtat aacggattga
    22801 aaatacattg tttataaaaa cagcaggcgc gcggtaatgg ctggattgcg acacggagtt
    22861 actttataat ccgctaccat ggccccttag ctcagtggtt agagcaggcg actcataatc
    22921 gcttggtcgc tggttcaagt ccagcagggg ccaccagaca tagcaaaggc tgacgagaaa
    22981 tcgtcagcct ttttcttttt atatatccgt tactttgcgt gccagtaagc cgccgcacgt
    23041 acccgctgtg ggtcatactg ttccgtttca aagcggcggc ttaaattctt aacgactttg
    23101 ccttcgccgg ttatccagat gaagtaatca tcggcaggga tttgcatctg cgccagacgc
    23161 gcatctaccg cctgctcatc atgtgccagc cattcgatat taaaaccatc aaggtgcgcg
    23221 agataatcct gacaggcgtt atcccgcacg ctaaccagcg cactaacttg cggtttaaca
    23281 gcaagtttgc tcaacgtttc caggcggcgg cgcagtgcgg gcattccgga ttcatcgcag
    23341 acatacagct gatacgcgta atcttccggc accaccagcg aaccgcgcgg acctgccacc
    23401 gtaagtttat cgcccggttt cgcctgcatc gcccagccgc tggcgacccc accgtcgtga
    23461 ataaaaaaat caatcgccag ttcatggcgt agttcgtcat acagcggcgt atagtcacgc
    23521 gacggtgggc gtgatccttc cggccagacg atgccctctt ccgttaccgt tggcggcaca
    23581 aagtgagcgt caggttgagg aaagaagagt ttgctgtgat cgtcaaagcc gcgcgatgta
    23641 aaaccgtcca gcgcctcgcc gccgaggaca atgcgctgaa aaccggcgct gatgcgctca
    23701 acgcgtagca cagtcagttc acggaagcgc agatcattgc gaacgcgctg tgggtagcgg
    23761 ggggtgttat tcattgttat cgccttcgtg atggtaatca gatatatcta aataaattcc
    23821 acaaatgata atgattgtta atcatgataa atgcaagtga tttgtagcac tgatatatct
    23881 gtagtctgat aagacgcgca acctcttatc aagcatcatt gctcaatgcc ggatgcggcg
    23941 tgaacgcctt atccggccta catgttgagt aaacctgcgt ttaaaaatca tttttgtact
    24001 tgcagtaatg ctcatatcag atataaatta gatatatcta attaagcaaa aggaggctga
    24061 tatgagccat catcacgaag ggtgttgtaa acatgaaggc cagccacgcc atgagggctg
    24121 ctgcaaaggt gagaagtcag aacacgagca ctgcggacac ggtcaccagc atgaacacgg
    24181 tcaatgctgc ggtggtcgcc acggtcgcgg cggcggtcgt cggcaacgtt tctttggtca
    24241 cggtgaattg cgtctggtga ttctggatat tctctcgcgc gatgacagcc acggttacga
    24301 gttgattaaa gcgattgaaa atctgaccca ggggaattac accccaagcc cgggcgtcat
    24361 ctacccgacg ctggattttc tgcaggagca gtcgctgatt accatccgcg aagaggaagg
    24421 tggtaagaag cagattgcgc tgaccgaaca aggcgcgcag tggctggaag aaaaccgcga
    24481 acaggtagag atgattgaag aacgcatcaa agcgcgttgc gttggcgctg cgctgcgcca
    24541 gaacccgcaa atgaagcggg cgctggataa ttttaaagcg gtgctggatt tatgcgtcaa
    24601 ccagagcgat atcactgatg cacaaataaa aaagatcatt gcggtgatcg accgcgccgc
    24661 ttttgatatt acccaactgg attaatcacc gattgccgga tgcggcgtac cctacacacc
    24721 ccgtaggcct gataagatgc gccagcatcg catcaggcat tgtgctccaa ccgccggatc
    24781 cggcataccg attaatgcag caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc
    24841 accatcgccg acgcctgcgc actctcttcc actaactcgg cgtttttctg ggtgatcagg
    24901 ttaagctcat ccactgcacg ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa
    24961 tggctaatct gagctatcaa ctgggtgacg ttttttacct gcgccacaat atcttccatc
    25021 gtccgtccgg cggcgtgtac ctgctgcgaa ccagattgca ccttatcagc actggcatca
    25081 atcagcttgc gaatatcgtt ggcagcattg gcgctgcggc tggctaaatg acgcacttcc
    25141 cccgccacca ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc caccgccgca
    25201 ttcagggcca gaatattggt ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca
    25261 atgcgctggg tactgtcggc aatatcgtcc attgtcttga tcaccgtagt catcgcctcc
    25321 ccgccctgca ctgccgcatt actggcggtg atagaaagtt tatcggcagc cgacgccgtg
    25381 gcagagttct gtttcaccga cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg
    25441 ttatcaactg tctgctgggt atgttcgttc agttcatcgg tgcctttcgc cagcgtctca
    25501 ctgccgtttc tgacactgga cacctggctt gagacatcat taattagcca acggcacatc
    25561 aggccaagct gccctaccgc gcgtaatgtc agccccagct catcgctgcg cttcagatgc
    25621 tcaacactat tacgttctcc ggtcgccact ttcagtgcct ggctggcgac attttctatc
    25681 ggacgtacaa tttgccattc aaaacaggca tttcccaaca acatcactaa tgcacagaag
    25741 atatacgtca ccaccggggc agcaacaaac cagagcatgg ccgccagcac gataaacatc
    25801 agggtcatca ctccacgcgc ccgccagcga agcggtaatg aaggcagttt acccagccag
    25861 cctttacgca ccaccaggcc tttatgaata cgcttaccgg tacgtccggc attcagcgct
    25921 ttgtacagcg gctccaccgc cgcgatctct tcatccgtcg cccgggtacg aatcgacata
    25981 tagccactga tttttccctc gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg
    26041 tcgccatttt tgcggcgatt tttcacgatg ccgctccagg gttccccttt tttcagggtg
    26101 aaccacatat ccgcaaacgc cgcttttggc atatccgggt gacgcaccat gttgtgcggt
    26161 tgcccctgta actcctgcaa ggtatagccg ctcacctgca caaaagtgtc attagcatga
    26221 gtgatatagc tttgcagatc ggtagtggac atcagagtgg tgtcgtccgc cagcggggta
    26281 ttttgctggg tgacatacgg atgagaagac atgttcgcgt cctgtgcagg ttatatggtt
    26341 gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac caattaattt
    26401 gatttagatc gcaatttgcg attaaagcac aaatctaatt ccttgattta aaatactttc
    26461 actctgttac tatacgaaaa cgttaattat cttgcccaaa aatcaggcaa ttattgccct
    26521 gaaagcgtgc atttgcgcag caatcatcaa atccataccc gacaaaaacc gtgcaaaata
    26581 acagcaaatg ttagcaaata gcattaaata ttgcgcaaat gataaccaca tttgtgttta
    26641 tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta aatcggtatc
    26701 atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc gcatcagctt
    26761 tagcgtgcag cgcccacgcc ctgaatctca ttgaaaagcg aacgttggat catgaggaga
    26821 tgaaagcact taaccgggag gtgattgaat acttcaaaga gcatgtcaat ccggggtttt
    26881 tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag tggcaagcgg
    26941 gaggtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc ctgggaggtt
    27001 ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta cagaatcaac
    27061 ttgcgaaaca accgcttcac agccaggagc tgcttgatcc gttacgggcg atgctggcga
    27121 aaacccttgc ggcgctaacg cccggtaaac tgaaatacag cttcttctgt aatagcggca
    27181 ccgaatccgt tgaagcggcg ctgaagctgg cgaaggctta tcagtcaccg cgcggcaagt
    27241 ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca
    27301 cggcgaaatc gaccttccgc aaaccgttta tgccactgct gccagggttt cgtcatgtac
    27361 cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa accggtgatg
    27421 atgttgctgc ggtgatcctc gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc
    27481 caggctatct caccgccgtg cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg
    27541 atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag catgagaacg
    27601 tacagccgga tatcctttgt ctggcaaaag cgctcggcgg cggcgtgatg ccgattggcg
    27661 caaccatcgc cactgaagag gtgttctcag tcctgttcga caatccgttc ctgcacacta
    27721 ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc
    27781 tggagcagaa cttaccggct caggctgaac aaaaaggcga tatgttgctg gacggtttcc
    27841 gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa gggatgttga
    27901 tggcgattga gtttgttgat aacgaaatcg gctataactt tgccagcgag atgttccgcc
    27961 agcgcgtact ggtggccgga acactcaaca acgctaaaac gatccgcatt gaaccgccac
    28021 tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg
    28081 ccatgcgagt cagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca
    28141 tccgatcttt ttatacgata cgtacgcccg caggcatcat ccgctccggc gttaacagca
    28201 cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac gtttcaccgc
    28261 gcattttcgc tttttgcaga ttgcataaga ccaccactgt ttttcccatc agctcttctt
    28321 cgctgtagta cggcaccaga ctggtcacgg tttgcagcgt tttttccccc acatcaacct
    28381 gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc tttccgacgc
    28441 gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc tcccttcaag
    28501 taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa aaatggagaa
    28561 tgcaggcgtg atcacattcg tgagccgctg tgttaccgtc acagcgtcaa agaaacgcgc
    28621 tttatttact gaaaacaggt gacccgataa gcacttcctc tacaatgggg gcgcacatca
    28681 gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga agctggcgta
    28741 tccctggcga cagtatccag ggtcttgaat gacgatccga cattgaatgt gaaagaagag
    28801 acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag tagcgcccgt
    28861 aaactccaga caggcgcagt caaccaacac catattctgg ctatctacag ctaccaacag
    28921 gagttggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga aactcagtgc
    28981 gaaaagctgg cgatcgaact gaccaactgt tatgaacaca acggcttacc agacattaaa
    29041 aacgtcaccg gtattttaat tgtcggcaaa cccacgtccg ccctgcgcgc cgccgcctgt
    29101 gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg
    29161 gtggatatcg atctggcacg tatcagtaaa gaaatcatcg atttctatat caatcagggc
    29221 gttaatcgta ttggttttat tggcggtgaa gacttacccg gcaaggcgga tattcgtgag
    29281 gtcgcctttg cggaatatgg tcgtctgaaa cacgtggtac gcgaagagga tatctggcgc
    29341 ggcggttttt ccagttcgtc gggctatgaa ctggcaaaac agatgctggc gcgggaagac
    29401 tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact gcgggcaatt
    29461 catgaacgtg gcctgaacat cccacaggat atttcgctta tcagcgttaa cgatatcccc
    29521 accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga gatgatggga
    29581 agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgttg
    29641 gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc cttacacact
    29701 gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc agttaaagta
    29761 aatgcattta cctgctattt tttagtaaaa attttactaa actccccagc aattacacaa
    29821 actaccatca ccataaatgg ttccgatttc tctctaccgg gaggccctat gaatcgctgg
    29881 gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat
    29941 gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt tctgtcctta
    30001 agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga agccttcacc
    30061 tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca aatggaaggt
    30121 cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtc
    30181 cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga cggctggcag
    30241 ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt ctacgttaac
    30301 ggtcagtatg tggggttcag caaaggcagt cgcctgaccg cagagtttga catcagcgcg
    30361 atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcaatgggc ggactctacc
    30421 tacgtggaag accaggatat gtggtggtcg gcggggatct tccgcgatgt ttatctgatc
    30481 ggaaaacaac tcacgcacat taacgatttc accgtgcgca ccgactttga cgaagcctat
    30541 tgcgatgcca cactttcctg cgaagtggtg ctggaaaatc tcgccgcctc ccctgtcgtc
    30601 acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag cgccattgat
    30661 catttggcaa ttgaaaaact gaccagcgcc cgctttgatt ttactgtcga acagccgcag
    30721 caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa agacgccgac
    30781 ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa agtgcgcgac
    30841 ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg tcacgacaac
    30901 gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct ccagttgatg
    30961 aagcagcaca acatcaactc cgtgcgtacc gctcactacc cgaacgatcc gcgtttttac
    31021 gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga atcgcacggc
    31081 tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga aaacgtctac
    31141 gtcgagcgca ttgttcgcca tattcacgcg cagaaaaacc atccgtcgat catcatctgg
    31201 tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca tgcggctaag
    31261 gcgctggatg acacgcgact ggtgcactac gaagaagatc gcgatgctga agtggtcgat
    31321 attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga atacccgcat
    31381 ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc gggcgggctg
    31441 acggagtacc agaacgtctt ctataagcac gattgcattc agggacatta tgtctgggaa
    31501 tggtgcgacc acgggatcca ggcgcaggat gacaacggca atgtctggta taaattcggc
    31561 ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt gatctattcc
    31621 gatcagacgc ctgggccggg cctgaaagag tacaaacagg ttatcgcgcc ggtaaaaatc
    31681 cacgcgttgg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg gtttaccacg
    31741 ctggatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct cgcgacgcag
    31801 cagattaaac tgtgcgacgt tgcaccgaac agcgaagccc ccttgcagat cacgctgccg
    31861 caactggacg ctcgtgaagc gtttctcaac attacggtga ccaaagattc ccgcacccgc
    31921 tacagcgaag ccggacaccc tatcgctact tatcagttcc cgctgaagga aaataccgcg
    31981 cagcctgtac ctttcgcgcc aaataatgcg cgtccgctga cgctggaaga cgatcgtttg
    32041 agctgcaccg ttcgcggcta caacttcgca atcaccttct caaaaatgag tggcaaaccg
    32101 acatcctggc aggtgaatgg cgagtcgctg ctgacccgcg agccaaagat caacttcttc
    32161 aagccaatga tcgacaacca caagcaggag tacgaagggc tgtggcagcc gaatcatttg
    32221 cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg tgaagtgttg
    32281 atcatcagcc gcacggttat tgccccaccg gtgtttgact tcgggatgcg ctgcacctac
    32341 atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga gcgttacggc
    32401 gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg cgaatacgat
    32461 caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca gcaggctaac
    32521 atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc cttcccacag
    32581 aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca cggcaatggt
    32641 ctgctggttg ttccgcagcg cccaattaac ttcagtgcct ggcactacac ccaggaaaac
    32701 atccacgcag cccagcactg taacgagctg cagcgcagtg atgacatcac cctgaatctc
    32761 gaccaccagt tgcctggcct cggctccaac tcctggggca gcgaggtgct ggactcctgg
    32821 cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc tggcggagaa
    32881 gctaccgcgc aaagccaggc gtcgtatgag ttcggcgcag ggttcttttc cacgaatttg
    32941 cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc cagatttacg
    33001 cctctggcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac aacattcagc
    33061 ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagacg gactccgcga
    33121 ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac ctgcaagggc
    33181 agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat tatcgtgatg
    33241 aaactgaccg cgaatattta aaaggctgcg gagaaaccgt agaggtccac gaagggcaaa
    33301 tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg gtcaaaaaag
    33361 tggttctcaa agtcaccatc gaagatggtt atttccataa caaataaaaa ctacggcggc
    33421 aaaaggagtt tgccgccacc gctaccctac tcattttcgg agatgtgtta tgtctgatac
    33481 caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgccg ccgtttacag
    33541 ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac cgatgttttt
    33601 cctcgcaaca attttttatt ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt
    33661 aaataaaaac tcggaagccg gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg
    33721 ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca cctcattatt
    33781 gccgcgcgtt attgcttacg cctcgtatgc attcctcggc tacgaatata ttatgacgcc
    33841 ggttgccacc accattatca gtatggtgct gttcgccttt tccacctggg tttctaccaa
    33901 cggggcgaaa atgctggggc caattacctc cgtcacttca acgctgatgc tgctgttaac
    33961 gctctcctac attttactgg caggtacggc actggttggc ggcgtacagc ctgctgaccc
    34021 catcactgtt gacgcgatga tcccaaactt taactgggcg ttcctcggcg ttaccacctg
    34081 gatctttatg gctgcaggtg gcgcggagtc cgtcgcggtg tacgttaacg acgtcaaagg
    34141 cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttattg gcgtactgta
    34201 ctccgtctcc tcggtactga ttaacgtctt cgtcagcagc aaagagttga agtttaccgg
    34261 cggatcggta caggtattcc acggcatggc ggcgtatttt ggtctaccgg aagcactgat
    34321 gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc tgatgtggac
    34381 ggcaacgccg gtgaaaattt tcttctccga aattccggaa ggcatctttg gtaagaaaac
    34441 cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt
    34501 catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc tgatgaatac
    34561 tattattaat atgactgctg cagcgtccat gcttccgccg ttattcatca tgctggctta
    34621 cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg gctcccgccg
    34681 caccggtatt atcgttgttt caatgctgat tgcgatattt gcagtagggt ttgtcgcttc
    34741 gacattcccg actggcgcga atattctgac catcattttt tataacgtcg gcggtattgt
    34801 tatcttcctc ggctttgcgt ggtggaaata cagtaaatat ataaagggat taacggctga
    34861 agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat aataaaaaat
    34921 gagcaggcat tttccctctt cctggatggg gagagggaaa atagtttctg cctttatatt
    34981 tttataatta caacgataaa aggctgtact ttttctttag ctcatggatt aacacaatga
    35041 aattaatcac tgcaccatgc agagcattac ttgccctgcc tttttgctac gctttttctg
    35101 cacttggcga agaagcacgt ccggcagaac atgacgacac aaaaaccccc gaaatgatct
    35161 cgacatcttc tccttcattt cgtttttacg gcgaattagg ggttggtggg tatatggatt
    35221 tagagggtga gaataaacat aaatacagcg acggcaccta tattgaaggt ggcctggaga
    35281 tgaagtacga ctcctggttc ggcctgattt acggcgaagg ctggaccgtg caggccgacc
    35341 acgacggtaa tgcctgggtg cctgaccata gctggggtgg tttcgaaggt gggattaacc
    35401 gtttctatgg cggttatcgt accaatgatg gcaccgaaat catgctcagt ctgcgtcagg
    35461 attcctcgct ggatgacctg caatggtggg gcgatttcac gcccgatctg ggctacgtca
    35521 ttcccaatac ccgcgacatt atgactgcgc tgaaggtaca gaacttaagc ggcaactttc
    35581 gttatagcgt taccgccaca cctgccggac atcatgacga aagcaaagcc tggctacatt
    35641 ttggcaagta cgatcgctac gacgacaaat acacctatcc ggcaatgatg aacggttaca
    35701 tccagtatga ccttgcagaa ggtatcacct ggatgaacgg tctggaaatc accgacggca
    35761 caggccaact ctatctcacg ggcctgctta cccctaactt tgccgctcgc gcctggcacc
    35821 ataccggacg cgccgacggg ctggacgtac cgggaagtga aagtgggatg atggtgagcg
    35881 ccatgtatga agcgttaaag ggcgtttatc tctccaccgc ttacacctac gccaaacatc
    35941 gccctgacca cgctgacgat gaaaccacct ctttcatgca gtttggtatc tggtacgaat
    36001 acggcggcgg acgtttcgca acggcttttg atagccgctt ctacatgaaa aatgcctctc
    36061 acgatcccag cgaccaactc ttcctgatgc aatattttta ctggtaataa ggacagtaat
    36121 ctcatgaaaa tcaaaactat tttaacacca gtagcctgtg ctctgctgat gagcttttcc
    36181 gcccatgccg ctaacgctga caattataaa aacgtgatta accgtactgg cgcgccgcag
    36241 tacatgaagg attacgatta cgacgatcac cagcgtttta atccgttttt cgatctcgga
    36301 gcctggcatg gtcatctgtt gccagacggc ccgaacacta tgggcggctt tccaggcgtt
    36361 gcgttgttga cggaagagta catcaacttt atggccagca atttcgaccg cctgaccgtc
    36421 tggcaggacg gcaagaaagt cgatttcacg ctggaggcat acagtattcc cggcgcgctg
    36481 gtgcaaaagt tgatatcgaa agatgtacag gtcgaaatga ttctgcgctt cgccacgccg
    36541 cgcacatcac tactggaaac caaaatcacc agcgataaac cgctggatct ggtgtgggat
    36601 ggcgaactgc tggaaaaact ggaagcgaaa gaagggaaac cgctttccga taaaaccatt
    36661 gctggcgagt accctgacta tcagcgcaaa atcagcgcca cccgcgatgg cctgaaagtt
    36721 acctttggca aagtgcgcgc cacctgggat ctgctgacct ccggtgaatc agaatatcag
    36781 gtgcataaat cattaccgat gcagactgaa atcaacggca atcgctttac cagtaaggcg
    36841 catatcaacg gatcgacgac gctctatacc acctattccc atctgctgac cgctcaggaa
    36901 gttagcaaag agcaaatgca gatccgcgat attctggcgc gtccggcgtt ttatctcacc
    36961 gcctcgcagc aacgttggga agaatacctg aagaaaggat taaccaatcc ggatgcgacg
    37021 ccggaacaga cgcgcgtcgc ggtgaaagcc atcgaaacgc tcaacggtaa ctggcgatcg
    37081 ccgggcggtg cggtgaaata taacaccgtc acgccgtcgg tgaccgggcg ctggttctcc
    37141 ggcaaccaga cctggccgtg ggatacctgg aagcaggcgt ttgcgatggc gcatttcaat
    37201 ccggacatcg ccaaagagaa tatccgcgcg gtcttctcct ggcagatcca gccaggcgac
    37261 cgcgtgcgtc cgcaggatgt gggctttgtc cccgacctga tagcgtggaa ccttagcccc
    37321 gagcgtggcg gcgatggcgg caactggaac gaacgtaata ccaaacccag ccttgccgcc
    37381 tggtcagtaa tggaagtgta taacgtcacc caggataagg cctggttggc agagatgtac
    37441 ccgaagctgg tggcctatca cgactggtgg ttacgtaacc gcgatcacaa cggcaacggc
    37501 gtgccggaat atggcgcgac ccgcgacaaa gcccacaaca ccgagagcgg cgagatgctg
    37561 tttacggtga aaaaaggcga caaagaggag acgcagtctg ggctgaacaa ctacgcccgt
    37621 gtggtggaga aaggccagta cgacagtctg gaaattccgg cacaggttgc tgcgtcgtgg
    37681 gaatcaggtc gtgatgacgc cgccgttttc gggtttatcg acaaagaaca gctggataaa
    37741 tatgtcgcta acggcggcaa acgtagcgac tggacggtga aattcgccga aaaccgcagt
    37801 caggacggaa cgttgctggg ctactcgcta ttgcaggagt cggtggatca ggccagctat
    37861 atgtacagcg ataaccatta tctggcggag atggcgacga ttctcggtaa gccggaagag
    37921 gccaaacgct atcgtcagtt agcacaacag ctcgcggact acatcaacac ttgtatgttc
    37981 gacccgacta cacagttcta ctatgacgtg cgtattgaag ataaaccgct ggcgaacggc
    38041 tgcgcgggca aaccgattat tgagcgcggt aaagggccgg aaagctggtc gccgctgttt
    38101 aacggtgagg caacgcaggc caatgccgac gcggtggtga aggtgatgct ggatccgaaa
    38161 gaattcaaca cctttgtccc gctgggaacg gcggcgttaa ccaatccggc ttttggcgcg
    38221 gatatctact ggcgcgggcg cgtatgggtg gatcagttct ggtttggtct gaaagggatg
    38281 gagcgttacg gttatcgtga tgatgccctg aagctggcgg atacgttctt ccggcacgcc
    38341 aaaggattaa ccgccgatgg cccaattcag gagaattaca acccgctcac tggctcgcaa
    38401 caaggcgcac caaatttctc ctggagtgcc gcgcatttgt atatgttgta taacgatttt
    38461 ttccgtaagc agtaatttgc aacggctggc ggatgcggcg tgaacgcctt atccgcccta
    38521 catgtgcaat cccccaggcc ggataaaacg cggcaagcgt cgcatccggc gttaccaccg
    38581 ggcgtattct ttttgaatcc catcacaaac cccgcactcc ccttttccct tttctccggc
    38641 gacggctaaa ttagaactca tccgaccaca taacaattat tttacatact ggacaatttt
    38701 atgagctacc cgtcgctgtt cgccccgctg gatttaggtt ttaccacgtt aaaaaaccgc
    38761 gtgttgatgg gctcaatgca caccgggctg gaggagtacc cggacggcgc tgaacggctg
    38821 gcagcgtttt atgccgaacg cgcccgtcac ggcgtggcgt tgattgtcag cggcggtatc
    38881 gcaccagatt taacaggcgt tggcatggaa ggcggtgcaa tgctcaatga cgccaaccag
    38941 atcccacacc atcgcaccat taccgaagcc gtacatcagg aaggcggcaa aatcgccctg
    39001 caaattttgc ataccgggcg ctacagctac caaccgcatc tggtcgctcc ctccgcattg
    39061 caggccccca tcaaccgttt cgttcctcac gaactcaccc atgaagagat cctgcaactg
    39121 atcgacgatt tcgcccactg cgcgcaattg gcacgggagg caggatacga cggtgtagag
    39181 gtgatggggt ccgaagggta tttgatcaac gaatttctga cgctgcgcac caatcagcgt
    39241 agtgaccagt ggggcggcga ttaccgcaac cggatgcggt ttgccgtaga agtagtgcgt
    39301 gcggtgcgcg aacgtgtcgg caacgacttc attattatct accgtttgtc gatgctcgac
    39361 ctggtcgaag gcggcggtac ttttgccgaa atggtagagc tggcgcaggc cattgaagcg
    39421 gcgggcgcga ccattatcaa caccggcatt ggctggcatg aagcgcggat tccgaccatc
    39481 gccacgcccg tgccgcgcgg cgcctttagc tgggtcacgc gcaaactgaa agggcacgtt
    39541 tcgctgccac tggtgaccac caaccggatt aacgatccgc aggttgccga cgacattctc
    39601 tcgcgcggtg atgcggacat ggtatcgatg gcgcgaccgt ttctcgctga tgcggagctg
    39661 ctatcaaaag cgcaatcggg acgagccgat gagatcaaca cttgtattgg ctgcaaccag
    39721 gcctgtctcg atcagatctt cgttggcaaa gtcacctcgt gcctggtgaa tcctcgcgcc
    39781 tgccacgaaa ccaaaatgcc aatccttcct gccgtgcaga aaaaaaatct ggcggtggtc
    39841 ggtgcgggac ctgctgggct ggcgtttgcc attaacgcgg cggcgcgtgg gcatcaggta
    39901 acattgtttg atgcgcacag cgagatcggc gggcagttta atatcgccaa acagatcccc
    39961 ggcaaagagg agttttacga aacgctgcgc tattaccgcc ggatgatcga agtgacgggt
    40021 gtaacgctaa aactcaatca caccgtgacg gcggatcagt tacaggcgtt cgatgaaacg
    40081 atcctcgcca gtgggatcgt gccgcgcgtt ccgcctatcg acgggatcga tcatccgaag
    40141 gtactgagtt atctcgatgt actgcgcgac aaagcgccgg tgggcaacaa agttgccatc
    40201 atcggttgtg gcggaattgg ttttgatacg gcgatgtatt taagtcagcc gggcgaatcc
    40261 accagccaga atatcgccgg gttctgtaat gagtggggaa tcgacagtag cctgcaacag
    40321 gccggaggtt tgagtccgca aggcatgcaa atcccccgta gcccacggca gattgtgatg
    40381 ctccagcgca aagccagcaa accaggacaa gggttaggca aaaccaccgg ctggatccat
    40441 cgcaccaccc tgctctcacg cggcgtgaaa atgatcccag gcgtaagtta ccagaagatc
    40501 gacgatgacg ggctgcatgt agtgatcaac ggcgaaacgc aggtactagc agtggacaat
    40561 gtggtgatct gcgcggggca agagccaaac cgcgcattgg cgcaaccgct gattgatagt
    40621 gggaaaactg tgcatttaat tggcggctgc gatgtagcta tggagctgga cgcacggcgg
    40681 gcaattgccc agggaacacg gctggcgctg gagatttaaa tcgttgtttg aagcgccgga
    40741 tgcgacgcat ccggcacgtc gcgttaatca ataaacaagg cgggagaaat accgaatcgc
    40801 gttgccagtt ttttagcgtg ttccagcgtt aatttccttt tcccgctcaa aacgcgtgac
    40861 accatcgatt tactgcctat ttccggaaga tcggaaaggg ttaaaccata ttgatccata
    40921 agggtacgaa tcacggctat accgccaggc atggcttgag ccatggcatt aaattccaca
    40981 aattcgggcg ctgattcttc ccacgcggtt attttggcac acaccagatc cagtaagggg
    41041 ttttcaggat cgttgagcag cagatgatct accagttcca gcgcctgggt gtattgttcc
    41101 tcgttctgaa tacccgccag aaaaggtgcc acagcagtta gcttttctcc tgcttgcaag
    41161 atgtcggcaa tcgcaatcat tttttcccct tagtacgatg aacggcggta aagaaatcgt
    41221 attctttatg cgtcataact tcacgtatgt agcacttttg cgattcaaaa aagaccattg
    41281 ctacaacacg taattcattg cccccgacat tgaaaacata atgcttatcc agatatttga
    41341 agttatccag agaagggaat actgctttta atgactcagg ttttttgaaa tatcccttag
    41401 caatcgtgtt ccccagagcc accaactccg ttttatgttg cgggtatttt tccgcagcat
    41461 ctttcaatgc tttttgagtt atcaggtgca ttcttcatca cgtccgttga caaattggca
    41521 atatgataac atccgttgac agattggcaa cgaatgaatt tatctgtgtg acggaagaat
    41581 aaacgtagaa aaacgctaaa caagagaatg tagaggatgg tgacaggaat tttttgtttt
    41641 atggaagcgc gattccagaa ctgggcagac attgccggat gcgacgcttg ccgcgtctta
    41701 tccgacctgg gggtaatgtg ctgaacgtat gccggataag gcgtttacgc cgcatccggc
    41761 aggtaaccct caatcttacc gacgacgccc cagcttcact gctttcagca ccacaaattt
    41821 attattcgtg gcgatggtgg tgcagttgcc gaaaattttc ttcagtttat ggaagtaatc
    41881 caagtgacgg ttggcaacga tatacagctc gccgttgatt ttcaggcagc ggcgggcgtg
    41941 gtggaacatc tcccaggcga cgttatcggt cagcgcatgt tgctgatgaa acggcgggtt
    42001 gcagagcaca gcattaaaac ggaaaggctc cacgccggaa agcgcgttgt tgatcataaa
    42061 ctcgcagcga tccagtgcct cgggcatgtt ggtttcaacg ttcaaacggc tggaagcaac
    42121 cgccatcggt gattcatcga caaacaccac tttcgcctgc gggtttttat cgagcaatgt
    42181 caggccaata acgccattac cgcaaccgag atcgacaatc tcaccttcga gattctctgg
    42241 cagatgttgc ataaagaagc gcgcgccaat atccagcccg gtgcgggaga agacattcgc
    42301 atggttgtgg atagtccagt cagtaccttc cagcttccag ctaacggtct gcggcgcatc
    42361 ggccaacggc ggttcgttga aggtgcagtt aatcaggcgc gctttcttcc atgccagcgt
    42421 ggtggtggtc gggccgagca ctttttcgaa caattccagc gtggaagtgt gaatgtcacg
    42481 ggctttggca ccagcaataa tgcgcgtatc tggcgtgacc actttgcgca gcgcgcgcag
    42541 ttgttgttcc agcaatgcca gtgttttcgg cactttgatc agcaccacac ccggctgttg
    42601 cgggtagtcg gcggtgctgt cgagaaactt cacgctcgac tcatcaatcc cgttgaggcg
    42661 taaattctcg cgcgtggcca gttcactgat gtatgagtcg ccaatgctgt acggcttatg
    42721 ttccgccagc acacagctta acgcaccaaa agcatcattc aggatcaaca ccgggccgcg
    42781 gatttctgtg tcgtccaact gttgcagcaa atactcatcc gccgcttccc acgcctgtag
    42841 cgggttaacg tcatccgtcg ccggaaaacg ttgtagtgtc agtgaacgga aaccgttgtc
    42901 taagtggctc atcggccctc ctgaatgata aaatttcgac gttgcccctg aaaagggtgc
    42961 gtgagtatac cctttttctc tttttcgtgg gcggttatga gcaatcttac ttatcttcag
    43021 ggttatcccg agcagctact ttcccaggtg cgaacgctga ttaacgaaca gcgtctgggc
    43081 gatgtgctgg caaaacgtta tccgggaaca cacgactacg ccaccgataa agccctctgg
    43141 caatatactc aggatcttaa aaatcagttt ctgcgcaatg ccccgccgat caataaagtg
    43201 atgtatgaca ataagatcca cgtgctgaaa aacgcgctcg ggttacatac tgccgtttcg
    43261 cgtgtgcagg gcggcaagct gaaagcaaaa gcagagatcc gcgtcgctac cgtgtttcgc
    43321 aacgcgccgg aaccgttttt gcgcatgatc gtggtgcacg agctggcgca cctgaaggag
    43381 aaagagcata acaaagcgtt ttaccagttg tgctgtcata tggaaccgca gtaccaccag
    43441 cttgagttcg acacccggct gtggctaacg cagttgtcgc ttgggcagga caaaatctga
    43501 aaatgatgtc aggcgttatt ttttcctcct ttagcgccta aaatcgatct cctccctttc
    43561 gttaaggata acgaccatgc tgcgcgcatt tgcccgcctt cttcttcgta tttgtttttc
    43621 tcgccggact cttaaaattg gctgtttatt gttgcttata gcaggagcga ccatttttat
    43681 tgccgatcgc gttatggtca acgccagcaa gcagctgacc tggagtgatg tcaacgccgt
    43741 tccggcgcgg aacgtgggtt tattgctggg ggcgaggccg ggtaatcgct actttacccg
    43801 acgtattgat agcgccgcag agttgtacca cgccggaaaa gtgaaatggc tattggtgag
    43861 cggcgataat ggacgtaaaa attacgatga agcgtcagga atgcagcaag ccttgatcgc
    43921 taaaggcgta ccggcaaaag ccatcttctg cgactatgcc ggattctcaa cgcttgattc
    43981 ggtggtgcgt gccaacaagg tgtttagtga aaaccatatc accattatct cgcaagaatt
    44041 tcataaccaa cgcgccatct ggctggcaaa acagtatggt atcgatgcta tcggttttaa
    44101 cgcgccagac ctgaatatga agcacggttt atatactcaa ctgcgggaaa aacttgccag
    44161 agtcagcgcg gtgattgatg cgaaaatcct ccatcgccag ccgaaatatc tcgggccgtt
    44221 ggttatgatt gggccgttta gtgagcatgg ctgtccggca aaagaataat gcgtatctgc
    44281 gcacgtcgaa gataaaaaag gcgtgctaca gtgacgacag aatcccttta tggagtatcc
    44341 acgcgttatg atacgtttcg ctgtgattgg tacgaactgg atcactcgcc agttcgtcga
    44401 ggccgcccat gagagcggta aatacaagtt aaccgccgtc tattcccgca gccttgaaca
    44461 ggcccagcac ttcgccaatg atttttctgt cgagcatctg tttacgtcgc tggaagcgat
    44521 ggcagaaagc gatgccattg acgcggtgta tattgccagc ccaaattccc tgcacttttc
    44581 ccagacacaa cttttcctta gtcacaaaat tcatgtgatt tgcgagaagc cactggcgtc
    44641 gaatcaggcg gaagtggatg ccgccattgc ctgtgcgcgg gaaaatcagg tggtgctgtt
    44701 tgaggcattt aaaacggcct acctgccgaa ctttcatctg ttacgccagg cgctgccgaa
    44761 agtcggcaaa ctgcgtaaag tctttttcaa ctattgccag tattcctcgc ggtatcagcg
    44821 ttacctggac ggagagaatc ccaacacctt caatccggca ttctccaacg gttcgattat
    44881 ggatatcggc ttttactgcc tggcgtcggc ggtggcatta ttcggtgaac cgaaaagcgt
    44941 gcaggcaacc gccagtttgc tggcaagcgg tgtggatgcc cacggcgtgg tggtgatgga
    45001 ttacggtgat ttcagcgtta cgttgcagca ctcaaaagtg agcgattctg tcctggcaag
    45061 cgagattcag ggcgaagcgg gatcgctggt gattgaaaaa ctctctgaat gccagaaagt
    45121 ctgctttgta ccgcgtggca gccagatgca ggatctcacc cagccacagc atattaatac
    45181 catgctctac gaagcagagc tgttcgctac cctggtggat gagcatctgg tgaatcatcc
    45241 agggctggcg gtcagtcgta tcaccgccaa actgctgact gagatccgcc gccagactgg
    45301 ggtgattttt ccggcagata acgtaaaact ataattgcca aagtaaaaca gtgtaaaagg
    45361 tatgtaacag accattgact gactgaatgg tctgtcatac tttgttacct gcaaagggga
    45421 gtaacttcat tgtcggtcga tcgtcattac ggtgtgtgaa aaaccacatc cggtcaccgg
    45481 gcaacccgaa aggaatacgc agacgtattc cttttttgtt gtaagtgaga ccttgccgga
    45541 aggcgaggtc tatgcataaa aagcagcggc tgacgtcttc cgacgttggc cgttttttta
    45601 tgtgtaagga acttctatga atactgtcgg cacgccgttg ctatggggcg gattcgctgt
    45661 tgttgtcacc attatgctgg ctatcgacct gttgttgcag gggcgtcgtg gggcacatgc
    45721 catgaccatg aaacaggctg cggcctggtc gctggtctgg gtgacgctgt cgttactgtt
    45781 taacgccgct ttctggtggt atctggtgca aaccgaaggt cgcgccgttg ccgatccaca
    45841 ggcactggcc tttctcacgg gttatctgat tgagaaatcg ctggcggtcg ataacgtctt
    45901 tgtctggctg atgttgttca gctatttctc tgttccggcg gcattacaac gccgcgtgct
    45961 ggtgtatggc gtactcgggg cgattgttct gcgtaccatc atgatcttca ctggtagctg
    46021 gctgatttca cagttcgact ggatcttgta tatcttcggt gccttcctgc tgtttaccgg
    46081 cgtgaagatg gcgctcgccc atgaagatga atcaggcatt ggcgacaagc cgctggtgcg
    46141 ctggctgcgc ggtcatttgc gcatgaccga caccatcgac aacgagcatt tctttgtgcg
    46201 taagaatggt ttgttgtatg ccacgccgct gatgctggtg ctgattctgg tggagttgag
    46261 cgacgtgatt ttcgccgtgg atagtattcc ggctatcttc gccgtgacca ccgatccgtt
    46321 tattgtgctg acctcaaacc tgtttgcgat cctcggcctg cgtgcgatgt atttcctgct
    46381 ggcaggtgtg gcagagcgtt tctcgatgct caaatatggt ctggcggtga ttctggtgtt
    46441 tatcggtatc aagatgctga ttgtcgactt ctaccatatt ccgatcgccg tctcgctggg
    46501 cgtggtgttt ggcattctgg tgatgacgtt tattatcaac gcctgggtga attatcggca
    46561 tgataagcag cgggttggat gatttttaat ctgcctaagc cgtgtaccct gtcatccaca
    46621 tgagcaccgt tttctccctc tccccaaaag gggcgagggg actgaccgag tacttttttg
    46681 acgttgtcat cagtctggaa gccgcacgtt gggctttatt ttcatgtcaa acaaatgtaa
    46741 ccattaagtt gcgaaatatg acttctcctt aaaatccagc atatttcgct tcccgaagct
    46801 gtaactttcc ttatactcga ccttgcaaac actttgttac atcctgaaag atgcgtcgac
    46861 agaacgcacc agggatgtgc gacaacacaa tgaaaggatc gaaaaatgac tacgcaacat
    46921 tcaccggggc tattccggcg tctggctcat ggcagcctgg taaaacaaat cctggtcggc
    46981 cttatactgg ggattcttct ggcatggatc tcaaaacccg cggcggaagc tgttggtctg
    47041 ttaggtactt tgttcgtcgg cgcactgaaa gccgttgccc ccatcctggt gttgatgctg
    47101 gtaatggcat ctattgctaa ccaccagcac gggcagaaaa ccaatatccg ccctattttg
    47161 ttcctctatc tgctgggcac cttctctgct gctctggccg cagtaatctt cagttttgcc
    47221 ttcccttcta ccctgcactt gtccagtagc gcgggtgata tttcgccgcc gtcaggcatt
    47281 gtagaagtga tgcgcgggct ggttatgagc atggtttcca accccattga tgcgctgctg
    47341 aaaggtaact acatcgggat cctggtgtgg gcaattggcc tcggtttcgc actgcgtcac
    47401 ggtaacgaaa ccaccaaaaa cctggtcaac gatatgtcga atgccgtcac ctttatggtg
    47461 aaactggtga ttcgcttcgc accgatcggt atttttggtc tggtttcttc taccctggca
    47521 accaccggtt tctccacgct gtggggctac gcgcaactgc tggtcgtgtt ggttggctgt
    47581 atgttactgg tggcgctggt ggttaacccc ttgctggtgt ggtggaaaat tcgtcgtaac
    47641 ccgttcccgc tggtgctgct gtgcctgcgc gaaagcggcg tgtatgcctt cttcacccgc
    47701 agctctgcgg cgaacattcc ggtgaatatg gcgctgtgtg aaaagctgaa tctggatcgc
    47761 gatacctatt ccgtttctat tccgctggga gccaccatca atatggcggg cgcagcaatc
    47821 accattaccg tgttgacgct ggctgcagtt aatacgctgg gtattccggt tgatctgccc
    47881 acagcgctgc tgttgagcgt agtggcttct ctgtgtgcct gtggcgcatc cggcgtggcg
    47941 gggggatctc tgttgctgat cccactggcc tgtaatatgt tcggtatttc gaacgatatc
    48001 gccatgcagg tggttgctgt cggctttatt atcggcgtat tgcaggactc ctgtgaaacc
    48061 gcgctgaact cgtcaactga cgtgctgttc actgcggcag cttgccaggc ggaagacgat
    48121 cgtctggcaa atagcgccct gcgtaactaa tacttagccc ctttcgtcta cggcggaagg
    48181 ggttttctcc actttaaacg gatcaattcc ccttctctgc atacgccaga aacgaatgat
    48241 attcaggcca ttcatcagca gaaaactgcc ttcaatcatc gtgccgccta tcgaccccgc
    48301 ccagaagttg tgaatcaccc agcaacacgt tgaaaaccac attacgcagc gcatggtcag
    48361 ccctttacag cggaacagcg cccaggtact gacaatcgtg ccgattaccg gcaatagttc
    48421 aacaggatga tggaacttcg cgaggccaat tccgccagtc agcacaataa aaatcgccat
    48481 tacccataag ctgcgcgtgc gtaaggtaat caatgtacga atggcattaa ggatggcact
    48541 ggcaccggca ggataggtgc ccagaagaaa aaaatgtacg ccaataacgg cgctatagac
    48601 cgaaagttgc tttttgaagc gacgttcgtc acgattgaaa aatgttgtga taccaatcag
    48661 aaaggcgatg acacccacgc cctgggccag ccaatacgcg gtcatgataa atccttagca
    48721 ggtatggaaa agcaaacggc gcttcacatt atgaaacgcc gttttttatt aacaactcat
    48781 ttcgacttta tagcgttacg ccgcttttaa agatcgccag ttcgcggaag tcgttacgct
    48841 cgttacaggt ttgcttaccg ttggcaaact caacgatggt gtcgataaat tcttccagca
    48901 actgcggcat cgctttaccg tggatcagct gacccgcgtc aaagtcgatc cagtgttttt
    48961 tcttcgccgc cagttcactg ttggtggcga ttttcaccgt cggcacaaat ccaccatacg
    49021 gcgtgccacg accagtactg aacagcacca tatggcagcc cgcacccgcc agggcgctgg
    49081 tcgctacggc atcgttaccc ggcgcactta acaagttcag ccccggcgtt ttcagacgct
    49141 cgccgtaacg cagcacgtca accacgacac tggaacccgc tttctgggta cagccaagcg
    49201 atttatcttc cagcgtggtg ataccgcctg ctttgttccc cggtgatggg ttttcgtaga
    49261 tcggctggtc atgggcaata aagtactgct tgaagtcatt gaccatggtg accagttttt
    49321 caaacgttgc ttcgtcgcgg caatggtcca tcagcaactg ctctgcgcca aacatctccg
    49381 gcacttcggt cagtacggta gtaccgccgt tagcaatcac gtagtcagag aaacgcccca
    49441 gcatcgggtt agcagtaata ccagaaagac cgtcagaacc accgcactcc agaccaaact
    49501 tcagttcgct gagtttgccc ggctcgcgtt tatcgttgcg catcacgcta tacagctgat
    49561 gcaaatgcgc gatcccggcc tcgatttcat catcctgctg ttggcagatc atgaaatgaa
    49621 cgcgttcagg atcgatatcg cccaacgttt cacggaatgc ggcaacctgg ttattttcgc
    49681 aacccagacc aatcaccagc actgcgcccg cgttcgggtg gcgcaccatg ttttgcagca
    49741 tggtgcgggt attgatgtga tcgtcgccca gttgtgagca gccgtaggtg tggctgaaga
    49801 ggaacacgcc gtcggtacct tcagcgttgt tggtctcttt caggaaacga ttttggatct
    49861 gccgcgcgat gccgttgaca cagcccacgg ttggcaggat ccacagctca ttacgcaccc
    49921 cgacatcacc gttagcgcga cgatagatct gcacttcacg atctgccgct tgcgcaggca
    49981 gatcctgaaa atcaggttga tagcgatact gatccagatc gctcagattc gtgcgcgtat
    50041 tgtgggcgtg aacgtgttcc cccgccgcaa tatccgccaa tgcataacca atcggcaggc
    50101 catatttaat gacgttggcc ccttttgcga tatccgttaa cgcaaattta tgtccacgag
    50161 caacatcctg gcgcagcgta acagtctggt tatcgacact gacttctgtg ccttcagcca
    50221 aatccgctaa agcaaccgcg acgttatcca gcgcatggat cttgaggtat tgcatatcaa
    50281 ccccagacct tagttcagtt caatggcgaa gtagtcacgc gcattgttaa agcaaatatt
    50341 tttcaccatc tcgcccagca ggttgatgtc cgccggtgct tcgcctgctt ccacccagcg
    50401 accgatcatc tggcacagaa tgcggcggaa gtattcgtga cgggtgtatg acaggaagct
    50461 acggctgtca gtcagcatgc cgacaaagcg gctcagcaga ccgagctgcg ccagttgggt
    50521 catctgacgt tccataccgt ctttctggtc gttaaaccac cagccggaac cgaactgcat
    50581 tttgcccggc ataccttcgc cctggaagtt accgatcatg gtgcccagca cttcgttatc
    50641 gcgcgggttc aggcagtaga gaatggtttt cggcagcagg ttttcttcgt tctgcttgct
    50701 cagcagctta gatagctctt ccgccatcgg acggtcgttg atggagtcaa agccgacatc
    50761 cggccccagc agtttgaact gacgcaggtt gttattacgc agtgcgccaa tgtggtactg
    50821 ctgtacccat ccgcgacgtg catattcggc accaaggaac accagcaccg cagttttgaa
    50881 ctgtgctact tcgtgctcgc tcagggtttc gcctgccaga cggcgcgcga ggatgctgtc
    50941 cagttccgct tcgttcgctt cagcaaacat caccacatcc agcgcgtggt cagacacttt
    51001 acagccgtga gcggcgaagt gatccagacg tttagtcagg gcagtttgca ggtcagcaaa
    51061 gcggcgaatg tcggtatcag aaacttcgcc cagcttcgcc atgtagtcgt taaaggtcgc
    51121 ctgttcaatg ttaaaggctt tgtccggacg ccagctcggc agcactttaa tggtgaaaga
    51181 gccgtctttg gcgatctctg cgtgatgctc cagagaatcg atcgggtcat cggtggtgcc
    51241 gaccattttc acgttcatct gctgcatgat gccgcgcgcg gagaagttat cctgcgccag
    51301 cagttcgtta cattcgttcc agatttcatc ggcagttgac ggagaaagca atttgccagt
    51361 gataccaaac ggacgacgca gttcgaggtg cgtccagtgg tataacgggt tgccgatagt
    51421 gtgcggaaca gtcgccgccc aggcgtcaaa tttttcacgg tcagacgcat caccggtgca
    51481 cagacgctcg gccacaccgt tggtacgcat agcgcgccat ttgtagtgat cgcccttcaa
    51541 ccagatgtca tacaggtttt taaaacgata gtcttccgca atctgctgcg gcggcaaatg
    51601 gcaatggtaa tcgaaaatcg gctggtcttt tgcgtagtcg tgatacagac ggcgggcaaa
    51661 ttcagtatct aacaggaaat cttcagtcat aaacggagtc attttcgtct tcctctcaac
    51721 gagtgagcta gcttgcttat ggtgcgatgc tgacaaagtt atcacaccaa tttccagagt
    51781 ccgaagatat tttcgtgagt tagatcaata aacgtagtta aaaaaattac tctcaaagtg
    51841 gtaaatctcg ctgcaggccg cgccagtact ggcctcgtcg tcgtcaggta atgtccctac
    51901 aaatattcgc acatttgtga tggctctcac cttttaaagt tgtatgacaa gttatctttc
    51961 tgccgtcgca aatcataagt cgacggaatg caaattgccg attcattcat ttgttagatg
    52021 aatcgggtta accggtacgg aagccgaatt agcacgaaac tttcatggca acgttcgggg
    52081 cgtgccggtt ttttttcggt tacccggtcg taactaacat cttcagcctc tggcgggatg
    52141 atggccgcgc ttcctgcgga tataacaaaa cgatgaggtt ttacatgcgt aaaattaaag
    52201 ggttacgttg gtatatgatc gcactggtga cgctcggcac cgtgcttggt tacctgacgc
    52261 gtaacactgt ggcggcagct gcgccaactc tgatggaaga gttaaacatc tccacccaac
    52321 agtattccta tatcatcgca gcgtattctg ctgcttatac ggtcatgcaa ccggtagcgg
    52381 gttatgtact ggatgtgctg ggtacgaaaa tcggttatgc aatgtttgct gtactgtggg
    52441 ccgtgttctg tggtgcaacc gcgctggcag gtagttgggg tggcctggcg gttgctcgtg
    52501 gtgcggtcgg tgccgcggaa gccgcgatga ttccggcggg tctgaaagcc agctccgaat
    52561 ggttcccggc aaaagagcgt tccatcgcgg taggttactt taacgtaggt tcttcgattg
    52621 gtgcgatgat tgcgccgccg ctggtggtat gggcaatcgt gatgcacagc tggcagatgg
    52681 catttatcat ctccggtgcg ttgagcttta tctgggcgat ggcatggctg attttctaca
    52741 agcatccgcg cgaccagaaa catctgaccg atgaagaacg cgactacatt attaatggtc
    52801 aggaagccca gcaccaggtt gacacagcga agaaaatgtc cgttggtcag atcctgcgta
    52861 accgtcagtt ctggggtatc gcgctgccgc gcttcctggc agaaccggca tggggtactt
    52921 ttaacgcgtg gatcccgctg ttcatgttta aagtttacgg ctttaacctg aaagagatcg
    52981 cgatgtttgc ctggatgccg atgctgtttg ctgacctcgg ttgtatcctc ggtggttacc
    53041 tgccaccact gttccagcgt tggtttggtg tgaacctgat cgtttcccgt aagatggtcg
    53101 taacgctggg tgcggtgctg atgattggcc cgggtatgat cggtctgttc accaacccgt
    53161 atgtcgcgat tatgctgctg tgtatcggtg gttttgctca ccaggcactg tctggcgcac
    53221 tgattacgct ctcgtctgac gtgttcggtc gtaacgaagt ggcgacggcg aacggtttaa
    53281 ccgggatgtc cgcatggctg gcaagtacgc tgtttgcact ggtagtcggt gcactggctg
    53341 acaccatcgg cttcagcccg ctgttcgcag ttctggcagt gttcgacctg ttaggtgcgc
    53401 tggttatctg gaccgtgttg cagaacaaac cagcgattga ggtggcgcag gaatcacata
    53461 acgatcccgc accgcaacat taaggcttcg tgtaagtttt agtttccctt caaagccgcc
    53521 ttctcaggcg gctttttcat cactgcgagt agagctaaac tcgcaaaagt ggtataacaa
    53581 atatagtctg ccgtatcatg cctggagcgc atatggaaat cactgaacca cgccgtttgt
    53641 atcaacaact tgccgctgat ctgaaagagc gcatcgaaca gggcgtctat ctggtgggtg
    53701 ataaactgcc tgccgaacgc tttattgccg atgaaaaaaa cgtcagccgc accgtggttc
    53761 gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa ggttcaggta
    53821 ttcatgtggt ttccaaccag ccgcgccatc agcaggcggc tgacaataat atggaattcg
    53881 ccaattacgg tccgtttgag ttgctccagg ctcgccagct catcgaaagt aatattgccg
    53941 agttcgcggc aactcaggtc acaaaacagg acatcatgaa attgatggcc attcaggagc
    54001 aggcacgcgg cgaacaatgc ttccgtgatt ccgagtggga tttgcagttc catattcagg
    54061 tagccctggc gacgcagaac tccgccctgg cggctatcgt tgaaaaaatg tggacccagc
    54121 gtagtcataa cccgtactgg aaaaaactgc acgaacacat tgatgcccgt accgtcgata
    54181 actggtgtga tgaccacgat caaatcctca aggcgctgat tcgcaaagat cctcatgctg
    54241 ctaagctggc aatgtggcag cacctggaaa acaccaagat catgttattt aacgaaacca
    54301 gcgacgattt cgagttcaat gccgaccgct atctgttcgc cgaaaacccg gtggtgcatc
    54361 tcgatacagc caccagcggc agtaaatgaa attcctgtcc gacaggcgct acagtgcgcc
    54421 tgtttgtctc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat cctctcgcct
    54481 ccctcccctg cattcagcaa aatcagactc catggacatg taattttgat aacgaacaac
    54541 gttgaccttt gttacaatta gattcaattt gaatttatgc ttttgaatgc tttcttatct
    54601 cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca gaacatacca
    54661 agacagcaat aacattgcgg tagtgcatca tttaaaacca gcgtggcgtt aaccgattca
    54721 ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg cgcaggattt
    54781 tgaaaccctg gccaatccgt cgatgattgg catgttgtat tttgtcttgt ttgtcatttt
    54841 gttccttgaa aacggcttgc ttccggcggc ctttttaccg ggcgacagtt tactggtact
    54901 ggtcggcgta ttgattgcga aaggcgcgat gggctatccg caaacgattc tgctgctgac
    54961 cgttgccgcc agcctcggtt gctgggtcag ctatattcag gggcgatggc tgggcaatac
    55021 ccgcaccgta caaaactggc tatctcattt acccgcgcat tatcatcaac gcgcacacca
    55081 tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg cgtttgtcag
    55141 aacactgctg ccgacgattg ccgggttatc agggctgaat aacgcgcgct ttcagttctt
    55201 caactggatg agtggtctgc tgtgggtgtt gatcctgaca actctgggtt acatgctcgg
    55261 caaaacaccg gtatttttaa agttcgagga ccagctgatg tcatgcctga tgctgctccc
    55321 ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtgttatgga aaaagaaata
    55381 tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtca cttcgccagc tagcctggtc
    55441 cggcgctgtt ttacttctgg tcagcacact attgctggcc tggtccgcgg ttcgccagca
    55501 agagtctacg ctggcgattc gtgccgttca tcaagggaca acgatgccag acggtttttc
    55561 aatctggcat caccttgacg ctcacggcat tcctttcaaa agtatcaccc ccaaaaacga
    55621 cactctgtta attacctttg actccagcga ccagagcgcc gccgcaaaag cggtcctcga
    55681 cagaacattg ccccatggct acatcattgc gcagcaggac aataacagtc aggctatgca
    55741 gtggctgacc cggttgcggg ataactctca tcgcttcgga taacttcctg ttatccgaaa
    55801 cagatcactc actttggtga tttcaccgta actgtctatg attaatgagg cggtggttaa
    55861 cacgaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat cacaatggaa
    55921 ggttcaagaa tgaaataccg catcgcttta gctgtttctc tctttgctct tagtgccggt
    55981 agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga gatcagctat
    56041 gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct gagtgaagtc
    56101 cgggccaact gttcagatag ccaactgcgt gccgatcatc agaagaaaat cgcaaagcag
    56161 aaagatgagg tggcggaacg ccagcaagat ttagccgagg cgaagcaaaa aggcgatgca
    56221 gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct gaaaaagctg
    56281 gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga aaactatgtc
    56341 gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg atacgctgga
    56401 agaggtgctt agctcatctg gcgagaagtc aaaagaagag ttgagtaaga ttcgtagcaa
    56461 agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg atgccattgc
    56521 caaacaaacc cgtgtcgcgg cggcgcgtgc cgatgagtat gtgcgtgaaa atccgtggac
    56581 gggcgtgggc attggcgcag caatcggtgt agtgctcggc gttctgctgt cgcgtcgtta
    56641 attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg catcgggcag
    56701 cgaattgttt ccatcatggt tgaaatggtg gagacacgtc tgcggctggc ggtggtggag
    56761 ctggaagagg aaaaagcgaa tctctttcaa cttttactga tgctgggcct gacgatgctt
    56821 ttcgctgcat ttggtcttat gagcctgatg gtgctaatta tctgggcggt tgatccgcaa
    56881 tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc actgattggc
    56941 ggcatctgga cgttacgtaa atcgcgtaag tctacgttgc tgcgccacac gcgccatgag
    57001 ctggcaaacg atcggcagct gctcgaggag gagtcccgtg agcagtaaag tcgaacgtga
    57061 acgacgtaag gcgcaactgc ttagccagat ccagcaacaa cggctggatc tttccgccac
    57121 tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata tgctgttgag
    57181 tctgcgctcc tgggcgctgg ttggcagtag cgtgatggct atctggacga ttcgccatcc
    57241 taatatgctg gtccgctggg ccagacgcgg ttttggtgta tggagcgcct ggcgtctggt
    57301 gaaaacaacc ctcaagcaac aacagcttcg cggttaactt cctctctggc cggagaaatt
    57361 ccggccttat tcctcaaatt ttttgaaaat atttgacagt tttccttgct aacaatcacg
    57421 cttcgccacg tttatgattc tctccatcga cagcaacgac gctaatagcg cgcccttgca
    57481 caaaaaaaca atcagcagcc tgagcggcgc agtggagagt atgatgaaaa aattagaaga
    57541 tgttggtgta ctggtagcgc gtattttaat gccgattctg tttattaccg caggctgggg
    57601 aaaaattact ggctacgcgg gtacccaaca atatatggaa gcaatgggcg tccccggctt
    57661 tatgctgccg ctggtgattc tgcttgagtt tggtggtggt ctggcaatcc tgttcggttt
    57721 cctgacacgt accacagccc tgtttactgc gggctttacg ctgctgacgg catttttatt
    57781 tcacagcaat tttgcagaag gcatgaactc gctgatgttt atgaaaaacc tgactatttc
    57841 tggcggattc ctgctgctgg caattaccgg tccaggcgca ttcagcatcg accgcctgct
    57901 gaataaaaag tggtaagcac gcgctatact taacgataaa aaacgagggg gaagctcctc
    57961 gtttttgcta ttggaggata gaaaaatggg tcaactgatt gacggcgtct ggcatgacac
    58021 ctggtacgat accaaatcca ccggcggtaa atttcaacgt tcagcttccg catttcgtaa
    58081 ctggctcact gccgatggcg cgccaggccc cactggcaaa ggcggttttg ccgcagagaa
    58141 agatcgttat catctctatg tttcactcgc ctgcccgtgg gcacaccgca cgctgatcat
    58201 gcgtaagctc aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct
    58261 ggaaaacggc tggacctttg atgacagttt tccgggagca agcggcgaca cgctctatca
    58321 acatgaattt ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac
    58381 tgttcccgtg ctgtgggaca aaaaaaacca caccatcgtc agcaacgaat cagcagaaat
    58441 catacgcatg ttcaataccg cgtttgatgc gctgggtgcg aaagctggag attactaccc
    58501 accagccctg caaccgaaaa ttgacgaact taacggctgg atttacgaca ccattaacaa
    58561 tggcgtgtat aaagccggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa
    58621 agtgtttgaa tcgctggcgc gactggaaca gattttaggt cagcaccgtt acctgaccgg
    58681 caaccagcta accgaagccg atattcgcct gtggaccacg ctggtgcgtt ttgatccagt
    58741 gtatgtgacc cacttcaagt gtgataagca ccgcatcagc gattacctga atctgtatgg
    58801 cttcctgcgc gatatctacc agatgccggg aattgccgaa acagtcaatt tcgatcacat
    58861 ccgtaatcat tacttccgca gccataagac catcaaccct acggggatta tttcaattgg
    58921 tccgtggcag gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc
    58981 accttacgag gtgcctctga taattagcta agtacgtgtt gttcatgtcg gatgcggcgt
    59041 aaacgcctta tcaaacctac aaaacattta tattcaatat gttgcaatga ccgcgcaggc
    59101 cagataagcg tagcgcatct ggcaattttg cgtttgccct tatccttcac agcgccttat
    59161 taattcatcg cgataatatt tactgccatt tcattcacgt ctattcttaa tttgctgctt
    59221 taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga aagtactaaa
    59281 aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt
    59341 caacatcatc tttacgttcg tgctggggct actggataaa atgttaggct ggcaacgtgc
    59401 cggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac catggtgggc
    59461 agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac tgctattctt
    59521 aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg gtacgccagg
    59581 cgaaaaccgc tttggaccag atccgaaact ggaacaagaa taataattaa gcaccttgta
    59641 aaaggtgctt aatcgattat tttccgctaa aaagtttggg aatttcccgc agacaccagg
    59701 atttcgcctc ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg
    59761 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
    59821 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
    59881 ccgttaagcg cggctgtttg tccagcaact gtacggtcaa caccgggcgc tcacgagcgg
    59941 tatccgccac cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct
    60001 cctgatgaat cgggtgatcc ggcgcggcaa catagacgtt cattaaggta tagagtttgc
    60061 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataacg atatccgccc
    60121 gcccctgctc cagccgttcc cacgcccccg ccagcacttc ggtgataatt gccagttggg
    60181 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
    60241 cctcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag
    60301 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
    60361 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
    60421 gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg
    60481 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
    60541 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
    60601 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
    60661 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
    60721 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctctctgcgc
    60781 gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccacgaac ctatcccaaa
    60841 gtcgatattt taaatgtgat tctggatgga gaagctgagt atcgcgacag cgaaggcaat
    60901 catgttcagg ccagcactgg tgaggtgttg ctgctctcta cccagccggg tgtcagctat
    60961 agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa tgcagctttg gctggatgcc
    61021 tgcccgcagc gagagaatcc gctgattcaa aagctggcgc ttaatatgag caagcagcaa
    61081 ttaatcgcct cgccagaagg tgggatggga agcctgcaat tgcgccagca agtgtggctg
    61141 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
    61201 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
    61261 ctaacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
    61321 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
    61381 gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc
    61441 tgctcgtacc gccaaaaatt ttggctatga agagatgttg agcgaactgg aagccatcgt
    61501 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
    61561 atgcggcgca aacgccttat ccggcacaat acccaaaacc gggcgtttat ctggccttgc
    61621 tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac
    61681 acaggttggc aatcgactgc tcaacatcat gcgccacaat cccttcattg ccggtcacgg
    61741 cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct
    61801 tcatcgcgca gctgttcgat gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac
    61861 tgctgatcgc catcgagatg gtttcataac gcccatccac cagccatgcc atcccggcag
    61921 cggcccccat tgctgcggtc gttgcggcac acagcgcaga caaacgcggt aactggttat
    61981 ggatgtaaat cgcgctcaaa tgcgaaagca tcagcgcacg cgccagccgt tcatcatccg
    62041 ctccgaagtg ttcagctacc accaccacgg gcattgttgc ggtgatcccc tggttacccg
    62101 agccggagtt actcattgcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc
    62161 tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc gcgcactgtt
    62221 tttccagcgt cgcaccaata tgcagccccc agttaccgct caaaccttcc tgcgataacg
    62281 cacaatttaa cttcgcagaa tcgagaataa agcggatcgc cgcaaacggg acttcattga
    62341 cgaacttcag gatctcagcc agcgtcgtcc tggaaagcac cgtcagcggc gactcttgct
    62401 cgccctctgc cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca
    62461 caatgttggt atgcccgccg acgatggtga cacacgccca tttctcaccg ttccagactt
    62521 tggcgcgtga gaagaggatt tcatcgcaag gttcctggat cttaactgag actttccccg
    62581 ccgccagcag tgctttggca tcggaaattg cctgcgcagt tgcgtctttc agcacttcca
    62641 gcccggcgtt agcatttcca cctaacgctc ccagcgccgc cgcaattggc agccccacca
    62701 ttcctgtacc gggaacggtg acacccagac cgttcttcat cagatttggc gaaacccagg
    62761 cttctacacg ttcaaccgga ccttccagtt cagccgcagc aaccgccgcc gccagcgcca
    62821 gtgaaatcgg ttcagtacac cccagcgccg gttttacttc ctcctgaacg gcgaggatgt
    62881 aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg
    62941 taaatcaaga aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca
    63001 ggtacaaaga catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg
    63061 ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg
    63121 gggcgttcag tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct
    63181 gaacgagatt ttcgttaatc ttctcggcag gtatcttgcg acgcaggatg ttcattacga
    63241 tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc attacagcaa
    63301 agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact
    63361 gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc
    63421 ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg
    63481 cgatattcat tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgagc
    63541 gataagagat caccatcggg cttaacgtct ggataaatag aatcgacgtc agggtaaacg
    63601 gcagcgtaat aatggcgttt ttcatcagca gccccagcgg cggtagcgaa ccgacgttat
    63661 acagatgcca cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca
    63721 gcaccatgcc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaggag atcgccacca
    63781 gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc
    63841 cgaaggtatg caggtaggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc
    63901 aaatcaccag cattacgaaa tagagtgcac ctaacaggat gccccagttt ttacctaaat
    63961 aaccgctgat gacgctcggg taatctttac attctggtga ttctgccagc gtattaataa
    64021 acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc
    64081 acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc
    64141 cgatactcat aatcacccag ccagtgtcag tactgtcgaa tttgatcgct tctcgccact
    64201 cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat
    64261 tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga
    64321 gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat
    64381 gatacgcgtg agatcagaga aaatcttcac aatcaagact tttaaatagc
//
LOCUS       DACADA010000007        62401 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.007, whole genome shotgun sequence.
ACCESSION   DACADA010000007 DACADA010000000
VERSION     DACADA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.007"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            167..1255
                     /gene="ompF"
                     /locus_tag="ISB17_000439"
     CDS             167..1255
                     /gene="ompF"
                     /locus_tag="ISB17_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAP1978418.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            1440..2630
                     /locus_tag="ISB17_000440"
     CDS             1440..2630
                     /locus_tag="ISB17_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAP1978419.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(2680..3327)
                     /gene="gloC"
                     /locus_tag="ISB17_000441"
     CDS             complement(2680..3327)
                     /gene="gloC"
                     /locus_tag="ISB17_000441"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAP1978420.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3354..3902)
                     /locus_tag="ISB17_000442"
     CDS             complement(3354..3902)
                     /locus_tag="ISB17_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAP1978421.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(4083..5930)
                     /gene="ldtD"
                     /locus_tag="ISB17_000443"
     CDS             complement(4083..5930)
                     /gene="ldtD"
                     /locus_tag="ISB17_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1978422.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(6191..10651)
                     /gene="mukB"
                     /locus_tag="ISB17_000444"
     CDS             complement(6191..10651)
                     /gene="mukB"
                     /locus_tag="ISB17_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP1978423.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVWKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(10651..11355)
                     /gene="mukE"
                     /locus_tag="ISB17_000445"
     CDS             complement(10651..11355)
                     /gene="mukE"
                     /locus_tag="ISB17_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAP1978424.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(11336..12658)
                     /gene="mukF"
                     /locus_tag="ISB17_000446"
     CDS             complement(11336..12658)
                     /gene="mukF"
                     /locus_tag="ISB17_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAP1978425.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(12655..13440)
                     /gene="cmoM"
                     /locus_tag="ISB17_000447"
     CDS             complement(12655..13440)
                     /gene="cmoM"
                     /locus_tag="ISB17_000447"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAP1978426.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            13576..14355
                     /gene="elyC"
                     /locus_tag="ISB17_000448"
     CDS             13576..14355
                     /gene="elyC"
                     /locus_tag="ISB17_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAP1978427.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(14332..15225)
                     /locus_tag="ISB17_000449"
     CDS             complement(14332..15225)
                     /locus_tag="ISB17_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAP1978428.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(15379..16125)
                     /gene="kdsB"
                     /locus_tag="ISB17_000450"
     CDS             complement(15379..16125)
                     /gene="kdsB"
                     /locus_tag="ISB17_000450"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP1978429.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(16122..16304)
                     /locus_tag="ISB17_000451"
     CDS             complement(16122..16304)
                     /locus_tag="ISB17_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAP1978430.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            complement(16356..17588)
                     /locus_tag="ISB17_000452"
     CDS             complement(16356..17588)
                     /locus_tag="ISB17_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAP1978431.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            complement(17625..18611)
                     /gene="lpxK"
                     /locus_tag="ISB17_000453"
     CDS             complement(17625..18611)
                     /gene="lpxK"
                     /locus_tag="ISB17_000453"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAP1978432.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(18608..20356)
                     /gene="msbA"
                     /locus_tag="ISB17_000454"
     CDS             complement(18608..20356)
                     /gene="msbA"
                     /locus_tag="ISB17_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAP1978433.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(20393..22657)
                     /locus_tag="ISB17_000455"
     CDS             complement(20393..22657)
                     /locus_tag="ISB17_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAP1978434.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(22863..23147)
                     /gene="ihfB"
                     /locus_tag="ISB17_000456"
     CDS             complement(22863..23147)
                     /gene="ihfB"
                     /locus_tag="ISB17_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAP1978435.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(23307..24980)
                     /gene="rpsA"
                     /locus_tag="ISB17_000457"
     CDS             complement(23307..24980)
                     /gene="rpsA"
                     /locus_tag="ISB17_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP1978436.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(25091..25774)
                     /gene="cmk"
                     /locus_tag="ISB17_000458"
     CDS             complement(25091..25774)
                     /gene="cmk"
                     /locus_tag="ISB17_000458"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP1978437.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(25947..26729)
                     /locus_tag="ISB17_000459"
     CDS             complement(25947..26729)
                     /locus_tag="ISB17_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1978438.1"
                     /translation="MYLKSNQKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNA
                     YKAATLSDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYK
                     VYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAI
                     VAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQ
                     GLVGSFEKLASLDGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(26873..27262)
                     /locus_tag="ISB17_000460"
     CDS             complement(26873..27262)
                     /locus_tag="ISB17_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1978439.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPEVADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGKLFADEPDADTEKKE"
     gene            complement(27234..27683)
                     /locus_tag="ISB17_000461"
     CDS             complement(27234..27683)
                     /locus_tag="ISB17_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HAP1978440.1"
                     /translation="MQKKRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKKKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLSTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(27685..27891)
                     /locus_tag="ISB17_000462"
     CDS             complement(27685..27891)
                     /locus_tag="ISB17_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978441.1"
                     /translation="MTTETIVCFLFWYMYAGSCSARLHRSLGYGRHYDTAHYILYMTA
                     VMLFWPVTLPAATDIVADRLKKRR"
     gene            complement(27881..28111)
                     /locus_tag="ISB17_000463"
     CDS             complement(27881..28111)
                     /locus_tag="ISB17_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978442.1"
                     /translation="MITLSGKSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHIEWGGIQNDY"
     gene            complement(28108..28791)
                     /locus_tag="ISB17_000464"
     CDS             complement(28108..28791)
                     /locus_tag="ISB17_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAP1978443.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAAYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(28788..29003)
                     /locus_tag="ISB17_000465"
     CDS             complement(28788..29003)
                     /locus_tag="ISB17_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978444.1"
                     /translation="MADILREITAWTLILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            complement(29018..29314)
                     /locus_tag="ISB17_000466"
     CDS             complement(29018..29314)
                     /locus_tag="ISB17_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978445.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLAEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            complement(29324..29596)
                     /locus_tag="ISB17_000467"
     CDS             complement(29324..29596)
                     /locus_tag="ISB17_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978446.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMATQA
                     IVATNGKEAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            complement(29885..30415)
                     /locus_tag="ISB17_000468"
     CDS             complement(29885..30415)
                     /locus_tag="ISB17_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAP1978447.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
                     SDIEDFSIIPFEQDVQ"
     gene            complement(30443..30712)
                     /locus_tag="ISB17_000469"
     CDS             complement(30443..30712)
                     /locus_tag="ISB17_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978448.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            complement(30715..31881)
                     /locus_tag="ISB17_000470"
     CDS             complement(30715..31881)
                     /locus_tag="ISB17_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1978449.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDAGA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            complement(31892..33661)
                     /locus_tag="ISB17_000471"
     CDS             complement(31892..33661)
                     /locus_tag="ISB17_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1978450.1"
                     /translation="MTPALTEKLVETARAARDAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSALTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFITAGRTDETTSEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVAAPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQMAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDTTLPAYMP
                     KRGQASDVRGPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            complement(33677..33994)
                     /locus_tag="ISB17_000472"
     CDS             complement(33677..33994)
                     /locus_tag="ISB17_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978451.1"
                     /translation="MNTNNTQNTLMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQETHPFVLTMEVMLMQQIRRLQNQSALRPVVAPQPVNTVVKTCDGETLCDLARKIAA
                     RIG"
     gene            complement(33994..34914)
                     /locus_tag="ISB17_000473"
     CDS             complement(33994..34914)
                     /locus_tag="ISB17_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HAP1978452.1"
                     /translation="MGRTKSPINTELNADVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERERIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFTNILENDLGLAPQVARR
                     MMQASVKFLGNGDEQPKRSALSVLGKTKLYELMVLDDEELDALADGGTVAGATLDDID
                     RMTSRELKAALREARETNAAQQQVLAGKDEKINELATKLEKKSRLQPPPPDEELKKLR
                     AEVTALAVEAESAIAVRLSSVFETLCEYCTKNMIDTPRDFMAGLVCQIERSVHALRET
                     FDLEAAPSGNDAPAWLTEPEPEINRPEDMQ"
     gene            complement(34925..35233)
                     /locus_tag="ISB17_000474"
     CDS             complement(34925..35233)
                     /locus_tag="ISB17_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1978453.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELATALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            complement(35286..35474)
                     /locus_tag="ISB17_000475"
     CDS             complement(35286..35474)
                     /locus_tag="ISB17_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1978454.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQTKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            35568..35924
                     /locus_tag="ISB17_000476"
     CDS             35568..35924
                     /locus_tag="ISB17_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1978455.1"
                     /translation="MSKDIGERVISVRKKAGLNQREFAARLGISNGGISQIENGKAMP
                     GGEFLLKMHQEFNVDVNWLLTGMSTDGMTRNQNQISPEKQKLIDTFDDMTPDQQRSFL
                     EIGRFYTQPKPGKIAG"
     gene            complement(36041..36805)
                     /locus_tag="ISB17_000477"
     CDS             complement(36041..36805)
                     /locus_tag="ISB17_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HAP1978456.1"
                     /translation="MKMQTLPIVPWIGGKRRLAKHILPLFPAHECYVEPFCGAAALYF
                     LKTPGKIEVINDINGELVNLYRVVKHHLEEFVRQFKWALVSRQIYKWLQITPEETLTD
                     IQRAARFYYLQKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTVIEHM
                     DWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIISVNDI
                     PQMREVFTGLNIQTVNINYSLAGKPTPRRELLICNF"
     gene            complement(36996..37211)
                     /locus_tag="ISB17_000478"
     CDS             complement(36996..37211)
                     /locus_tag="ISB17_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978457.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLYRGELSVCRAAFHFQGI
                     QKQFDTSVFAEGITYALDRIRSENRPG"
     gene            37210..37614
                     /locus_tag="ISB17_000479"
     CDS             37210..37614
                     /locus_tag="ISB17_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978458.1"
                     /translation="MMHSLNDDFYVLTGKIFCRLLNTHPAPQVLIYSGFFDIPDQVDH
                     DQKKCRCFIDTLIWLRDNSYIRYSQLLAVGAADVVLTEKTLCLLNAMPTFLQPRPVPF
                     KESLLDAVKTGENAVIAECVSRIMRYVFNERK"
     gene            complement(37590..38318)
                     /locus_tag="ISB17_000480"
     CDS             complement(37590..38318)
                     /locus_tag="ISB17_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978459.1"
                     /translation="MTQTLNPVITVTVSGPVGSGKSYVLARIEEVVKQEFGNSVIVDA
                     ADVDGERRMSGDDLSTWQKPRAGTVIRLEEYTGQGLLSYGEERICRTNELLDVICPTG
                     RQPWILPDALHATIAALMTVYDQELLMKELRKLTGQSTECCSSPKYGKQEHIPINQPP
                     RFFDEYLLTPENKAEHEEIKAALFREIRKIAEMQLEGGLDRVMARGEWASLFERVSPE
                     LLPLALAIGIMQTADKPHLRSLNT"
     gene            38449..38799
                     /locus_tag="ISB17_000481"
     CDS             38449..38799
                     /locus_tag="ISB17_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978460.1"
                     /translation="MKNLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHDDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            38802..39542
                     /locus_tag="ISB17_000482"
     CDS             38802..39542
                     /locus_tag="ISB17_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1978461.1"
                     /translation="MNWPQITWIVCMSLKLAFEAFRVFIRTERLSVRAGTFLVHMAWV
                     FFVAMLLWCGGFFSQACAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRPDAISPAGAQGLAQFMPATADWISQLIPMLSSREPFNPAWAIRALVSYDRWLWQRV
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILRELAPRYLTWGGSSCVASG"
     gene            39526..40176
                     /locus_tag="ISB17_000483"
     CDS             39526..40176
                     /locus_tag="ISB17_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978462.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLVGLYAMGYRSGHDSAKRDGDT
                     ALSQLQSAFDAYKTEQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAWQCRRWRNQINALLDEREGLQK"
     gene            40173..40499
                     /locus_tag="ISB17_000484"
     CDS             40173..40499
                     /locus_tag="ISB17_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978463.1"
                     /translation="MTLQVEFWTVVSFLLTFMRFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            40499..40810
                     /locus_tag="ISB17_000485"
     CDS             40499..40810
                     /locus_tag="ISB17_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978464.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVELEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            40810..41355
                     /locus_tag="ISB17_000486"
     CDS             40810..41355
                     /locus_tag="ISB17_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HAP1978465.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            41415..42947
                     /locus_tag="ISB17_000487"
     CDS             41415..42947
                     /locus_tag="ISB17_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978466.1"
                     /translation="MDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIEKSR
                     RTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAASEI
                     EEGFWEEDDDDKHIKTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQLDE
                     LLKAALAMLIWGGKVRVISTHDGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSEGLF
                     RRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESRMSA
                     DTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRSGDL
                     SVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNGAYL
                     AEYAMQRYGSGRVQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAVQVV
                     NGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAARMLE
                     GF"
     gene            42947..44443
                     /locus_tag="ISB17_000488"
     CDS             42947..44443
                     /locus_tag="ISB17_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HAP1978467.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRSWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELRAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETPPSERQNNMLPLSFAEAIDADIQAQQQLDDALDILMNGGALNGTLEPVLAPLFRRV
                     ENGVNPSELLGELAELYPQMNTDDLQERLARILFVANIRGRLHERDNG"
     gene            44424..45245
                     /locus_tag="ISB17_000489"
     CDS             44424..45245
                     /locus_tag="ISB17_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP1978468.1"
                     /translation="MSVTTAELAYCMTLPPKRAINYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQELEPVLKRKGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            45248..45706
                     /locus_tag="ISB17_000490"
     CDS             45248..45706
                     /locus_tag="ISB17_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP1978469.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERDGQTLQDTGRLMGSVSTDHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            45921..47036
                     /locus_tag="ISB17_000491"
     CDS             45921..47036
                     /locus_tag="ISB17_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP1978470.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            47051..48004
                     /locus_tag="ISB17_000492"
     CDS             47051..48004
                     /locus_tag="ISB17_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978471.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            48014..48352
                     /locus_tag="ISB17_000493"
     CDS             48014..48352
                     /locus_tag="ISB17_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HAP1978472.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            48354..48800
                     /locus_tag="ISB17_000494"
     CDS             48354..48800
                     /locus_tag="ISB17_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HAP1978473.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            48800..49264
                     /locus_tag="ISB17_000495"
     CDS             48800..49264
                     /locus_tag="ISB17_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978474.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            49261..49515
                     /locus_tag="ISB17_000496"
     CDS             49261..49515
                     /locus_tag="ISB17_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978475.1"
                     /translation="MMKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVA
                     LKHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            49505..50932
                     /locus_tag="ISB17_000497"
     CDS             49505..50932
                     /locus_tag="ISB17_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAP1978476.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            50932..51453
                     /locus_tag="ISB17_000498"
     CDS             50932..51453
                     /locus_tag="ISB17_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP1978477.1"
                     /translation="MSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKALG
                     MVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDEI
                     PLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVGG
                     VDQLTDYRINIGG"
     gene            51456..51737
                     /locus_tag="ISB17_000499"
     CDS             51456..51737
                     /locus_tag="ISB17_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978478.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGIGKSEGKTLLIAFLRNCKGFRTC"
     gene            51835..52170
                     /locus_tag="ISB17_000500"
     CDS             51835..52170
                     /locus_tag="ISB17_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP1978479.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            52094..52252
                     /locus_tag="ISB17_000501"
     CDS             52094..52252
                     /locus_tag="ISB17_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP1978480.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            52328..55279
                     /locus_tag="ISB17_000502"
     CDS             52328..55279
                     /locus_tag="ISB17_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1978481.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAMGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQDLLPLIQGTKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVNVWKTAVLLRTNWHRLTTALGEGGKLRWLVTGFSRLTSGGLKLSKVLAGSLVRGF
                     MSAARAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGAVSGWFKQRWADIQEAFNGG
                     IAGTGKLLINWSPAGLLYKAFAAALKYFGVTLPAKFTDFGGHLIDGLINGIKNKWGSL
                     KSSVTGMGDSISSWFKQCWADIQEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLG
                     VDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFM
                     GFGDNIAQGAAIGLQRTTPLAALAGQRMASELLPRMPVSIQGPEIRDNTSGVRFSMPL
                     PDINGFMSSAKNAIGAVINSLSSMPAIPLSTRASILPGQRLANEMTPDVPRIPSPEIL
                     AAGYSGRGAAATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLHELEKMLER
                     LLAQKQRKEYR"
     gene            55279..56163
                     /locus_tag="ISB17_000503"
     CDS             55279..56163
                     /locus_tag="ISB17_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1978482.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQITLVFHQHYCVPDVELARLRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAVGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            56160..56375
                     /locus_tag="ISB17_000504"
     CDS             56160..56375
                     /locus_tag="ISB17_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011410692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978483.1"
                     /translation="MMFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVL
                     PSGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            56363..57517
                     /locus_tag="ISB17_000505"
     CDS             56363..57517
                     /locus_tag="ISB17_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP1978484.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVSFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            57514..58110
                     /locus_tag="ISB17_000506"
     CDS             57514..58110
                     /locus_tag="ISB17_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1978485.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLIIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGEGVTATIHGNIEIKGNVHATGSMLSDGENSSHHSH"
     gene            58165..58512
                     /locus_tag="ISB17_000507"
     CDS             58165..58512
                     /locus_tag="ISB17_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAP1978486.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            58503..59606
                     /locus_tag="ISB17_000508"
     CDS             58503..59606
                     /locus_tag="ISB17_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAP1978487.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTATCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVNLLVSNLMASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMTGVSDD"
     gene            59599..60177
                     /locus_tag="ISB17_000509"
     CDS             59599..60177
                     /locus_tag="ISB17_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1978488.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            60180..61460
                     /locus_tag="ISB17_000510"
     CDS             60180..61460
                     /locus_tag="ISB17_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1978489.1"
                     /translation="MGNLNETEKWEENIYQLETSDPVLGGADGISNRAPRQLANRTKW
                     LKKKTEEAAQSLAEHVRSRNHPDATLTAKGFTQLSSATNSTSETLAATPKAVKAAYDL
                     AAGKAPASHTHPWSQITGVPAASLTAKGTVQLSSATDSQSETEAATPKAVKAAYDLAA
                     GKAPVSHTHPWSQITGVPAASLTAKGTVQLSSAINSTSEILAATPKAVKAAYDLANGK
                     QPADATLTALAGLATAADRLPYFTGADRAELATLTAIGRAIIAKGSIKDVLNYLGLGE
                     GSALPVGVPVPWPTATPPAGWLQCNGATFTKEQYPVLVRVYPTLRLPDLRGEFIRGWD
                     GGRKVDTGRALLSFQEGTIVSGFDDNDTGDISSLGSTQYGFGDALTSNQLGVLNGKKW
                     IFDASSKGAQRYDWWAYVSARPRNIAFNYIVRAA"
     gene            complement(61467..62081)
                     /locus_tag="ISB17_000511"
     CDS             complement(61467..62081)
                     /locus_tag="ISB17_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1978490.1"
                     /translation="MSNTAVLDENGIATVAGDITVYHYDEETREYTSSSVEYLALGVG
                     TPAHSCADAPPEAISGYVVCRTATLNGWEHVPDHRGETVYSTENGNPVLITQPGDYPA
                     DTTTKQPATPWDTWNGEAWVTDTERQRAAELEVARQQRQQRVKQAMASVDLINLKLRA
                     GRSLKPEETAKLNAVLDYIDELNALDISKAPEISWPEAPLALAG"
     gene            complement(62081..>62401)
                     /locus_tag="ISB17_000512"
     CDS             complement(62081..>62401)
                     /locus_tag="ISB17_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978491.1"
                     /translation="KVDTGRKLLSAQGATLLRTAMLDYYNQDTTGTSGIVGMGFNNED
                     SITDLREGSFKMPDGTTFSDPVVAMSDNGMQATILTSIRSGYAKGITVRPRSIALNYI
                     VRAV"
BASE COUNT        14276 a        16833 c        16585 g        14707 t
ORIGIN      
        1 atctttgtag cactttcacg gtagcgaaac gttagtttga atggaaagat gcctgcagac
       61 acataaagac accaaactct catcaatagt tccgtaaatt tttattgaca gaacttattg
      121 acggcagtgg caggtgtcat aaaaaaaacc atgagggtaa taaataatga tgaagcgcaa
      181 tattctggca gtgatcgtcc ctgctctgtt agtagcaggt actgcaaacg ctgcagaaat
      241 ctataacaaa gatggcaaca aagtagatct gtacggtaaa gctgtcggtc tgcattattt
      301 ctccaagggt aacggtgaag acagttacgg tggcaatggc gacatgacct atgcccgtct
      361 tggttttaaa ggggaaactc aaatcaattc cgatctgacc ggttatggtc agtgggaata
      421 taacttccag ggtaacaact ctgaaggcgc tgacgctcaa actggtaaca aaacgcgtct
      481 ggcattcgcg ggtcttaaat acgctgacgt tggttctttc gattacggcc gtaactacgg
      541 tgtggtttat gatgcactgg gttacaccga tatgctgcca gaatttggtg gtgatactgc
      601 atacagcgat gacttcttcg ttggtcgtgt tggcggcgtt gctacctatc gtaactccaa
      661 cttctttggt ctggttgatg gtctgaactt tgctgttcag tacctgggta aaaacgagcg
      721 tgacactgca cgccgctcta acggcgacgg tgttggcggt tctatcagct acgaatacga
      781 aggctttggt atcgttggtg cttatggtgc cgctgaccgt accaacctgc aggaagagtc
      841 tccccttggc aaaggcaaaa aagctgaaca gtgggctact ggtctgaagt atgacgcgaa
      901 caacatctac ctggcagcga actacggtga aacccgtaac gctacgccga tcactaacaa
      961 atttacaaac accagcggct tcgccaacaa aacgcaagac gttctgttag ttgcgcaata
     1021 ccagttcgat ttcggtctgc gtccgtccat cgcttacact aaatctaaag cgaaagacgt
     1081 agacggtatc ggtgatgttg atctggtgaa ctactttgaa gtgggcgcaa cctactactt
     1141 caacaaaaac atgtccacct atgttgacta catcatcaac cagatcgatt ctgacaacaa
     1201 gttgggcgta ggttccgacg ataccgttgc tgtgggtatc gtttaccagt tctaatagca
     1261 cacctctttg ttaaatgccg aaaaaacagg actttggtcc tgtttttttt atgccttcca
     1321 gagcaatctc acgtcttgca aaaacagcct gcgttttcat cagtaatagt tggaattttg
     1381 taaatctccc gttaccctga tagcggactt cccttctgta accataatgg aacctcgtca
     1441 tgtttgagaa cattaccgcc gctcctgccg acccgattct gggcctggcc gatctgtttc
     1501 gtgccgatga acgtcccggc aaaattaacc tcgggattgg tgtctataaa gatgagacgg
     1561 gtaaaacccc ggtactgacc agcgtgaaaa aggctgaaca gtatctgctc gaaaatgaaa
     1621 ccaccaaaaa ttacctcggc attgacggca tccctgaatt tggtcgctgc actcaggaac
     1681 tgctgtttgg taaaggtagc gccctgatca atgacaaacg tgctcgcacg gcacagactc
     1741 cgggtggcac tggcgcacta cgcgtggctg ccgatttcct ggcaaaaaat accagcgtta
     1801 agcgtgtgtg ggtgagcaac ccaagctggc cgaaccataa gagcgtcttt aactctgcag
     1861 gtctggaagt tcgtgaatac gcttattatg atgcggaaaa tcacactctt gacttcgatg
     1921 cactgattaa cagcctgaac gaagcccagg ctggcgacgt agtgctgttc catggctgct
     1981 gccataaccc aaccggcatc gaccctacgc tggaacaatg gcagacactg gcacaactct
     2041 ccgttgagaa aggctggtta ccgctgtttg acttcgctta ccagggtttt gctcggggtc
     2101 tggaagaaga tgctgaagga ctgcgcgctt tcgcggctct gcataaggag ctgattgttg
     2161 ccagttccta ctctaaaaac tttggcctgt acaacgagcg tgttggcgct tgtactctgg
     2221 ttgctgctga cagtgaaact gttgaacgtg cattcagcca aatgaaagcg gcgattcgtg
     2281 ctaactactc taacccacca gcacacggcg cttctgttgt tgccaccatc ctgagcaacg
     2341 atgcgttacg tgcgatttgg gaacaagaac tgactgatat gcgccagcgt attcagcgta
     2401 tgcgtcagtt gttcgtcaat acgctgcagg aaaaaggcgc aaatcgcgac ttcagcttta
     2461 tcatcaaaca gaacggcatg ttctccttca gtggcctgac aaaagaacaa gtgctgcgtc
     2521 tgcgcgaaga gtttggcgta tatgctgttg cttctggtcg cgtaaacgtg gccgggatga
     2581 caccagataa catggcaccg ctgtgcgaag cgattgtggc agtgctgtaa gcgttaaaac
     2641 cgatgaagcc cgcttgaaag cgggcttttt tatgcgcctt taccagacag gcatttcgtc
     2701 ttgcaagaag ggattatgca ggcgctcata accgagagtg gataatggtc cgtgacccgg
     2761 aataaatgtc acgtcatccc ccagtggcag caatttatct ttgattgaag aaatcagttg
     2821 attgtgatcg ccacgcggga aatcactgcg ccctactccg cctttgaaaa taacatcgcc
     2881 agaaatgagc agccttgcct gctcattaaa aaacaccaca tgacccggcg tatgcccggg
     2941 gcaatgtaac acctgtaaag tcacattccc tatgctgatg gtatcgcctt cgttcagcca
     3001 acgatctggc gtcagcggct ggcactcttc cagaccaaac atacgacttt gcgcaggcaa
     3061 gccttgcagc cagaactcat cttctttttc cgggccaaaa accggcacgc cgtaatgttg
     3121 cgccagttcc gccgctgcgc caacgtggtc cagatgacca tgagtcagca ggatctgcat
     3181 cagtgtcagg ccgctggcat caacttcctg tttgattttt tccgcatcgc cgccaggatc
     3241 gaccagtgcg gccagacggg tttgttcaca ccagatcaat gaacagttct gggagaatgc
     3301 ggtgaccgga ataatacgat agttcatact gcccctgttt cgttaagcga ttactaccag
     3361 tgccgtgctg gcccggtatc aatatgcaca aagttactac gtgggtaata tcctacacca
     3421 cctgcgcgca tagataacgc ggctttgcga atattgctta acgcgatacc ttcaatgtgg
     3481 aaatccatcg cctggccttt ggtgtgatag cttttcttcg ctactccacg gctgcgggcg
     3541 cgtagttcat tgttggtatc aatggaacga taaccggaaa tgagttgcac cggtttgcgg
     3601 gtgcctaaca acccttgcag gcgatacaac tggtcgaata atcctgggtc gatggacttt
     3661 attttgttcg cgcggtaatc gcggaaaaaa tggttaagtt ttgccaattc ttcctgaata
     3721 tagcctctgc catcgaaaaa ctccgctttg attgactctc cggtatgaag attattgagt
     3781 gtcaaaatgc gcgggcgtgg ggtagagagt gttgcaaacg ccggggtcgg caggatggcg
     3841 gcaccgagtg caacgccacc aagcgccagc agtttgcggc gattagcgtc gaatttgtcc
     3901 atgataatca agtctacagg tcaatgttat cgtttatatg cacttctggc gcacaagagg
     3961 caccttaacc gcccgtaaag aagacgtcaa gatgactcaa ccccagcagt gacgggggct
     4021 gcagagaatc gcccccgatc atgctaatta ttacgacaac tgatttcccc gaactgcttc
     4081 atttacctga ttaattgttc cgctttcgat acaatttgcg agctggatcg cgcaggcaga
     4141 tcataattgt aaatatctgt acgatactgg gtacgaccat cagcaccaac aaaggccgtc
     4201 agatagtaga gattcaccgg aatcgactgc cgaatattga cgtaacgcgt atcgccctgc
     4261 ttcagcgcat cagaaatacg tttgtcattc catcctgcat cctgcaacag catattcgcc
     4321 agatcggaag ctttattcac tcgtacacag cctgagctca atgcgcgtgt atcacgtttg
     4381 aacagattgt ggttcggcgt gtcatgcaaa taaatggcct ctgaactcgg catattgaat
     4441 ttatagcgcc ccagcgagtt ccgtgggcct ggagcctgct ggaaacggaa cggtaaattc
     4501 gaggccgtga ttgtagacca gtcaacctgc catgggtcaa tcgcttctct gctgttccag
     4561 ccgcgcatca ccgtatagcc atggctttcg agatatcccg gatcgttgcg cactttcggc
     4621 agaatatctt tgcgtgccag agttggtggt acgttccacg gcgggtttac cactacgttg
     4681 ttaagtgcac tgctcatcat cggcgttttg cgatcggggc gaccgacaat gactcgcgaa
     4741 tccagcacct gattgccgtt ctgatagtag accagcgaat aggctggaat gttaaccatg
     4801 atcccggtag aaagctctgt tggcagcaag cgcaatcgct ggatgttgag tgccaacacg
     4861 ccagcacgct gggcgggcgt tacgtttaac cagtcacgcg ttgccgggcc aatagcacca
     4921 tctgctccca atccttgcca tgcctgaaaa cgtttaacgg cttccaccag ttcattatcg
     4981 taggcggcgc gaacggcagg cgcaggttta ctacgagacg tagtttgttt gtccatcagc
     5041 ttagtttctg ctgtttcaac agtaacagcc gatgggctga ctaccgcgtc agttggcgtg
     5101 tcatcgccag gtagcgtaat tttcggtccc ccgtccaaca tgcctgtacg ttgcaatatt
     5161 tcgcgcaacg ccggtacgtc gttactccac tgtcctgggc gcaacgttgc tttaccggtc
     5221 agttgaggcc acggtttggt gtcactgagt aaggccagta acgattcatg catcgccgca
     5281 tattgcggat gctgcggtgc cagtcctgca acaaacgtcg gcaattgacc tttatccagc
     5341 gccagctgcc attggttaat caccgaaagc ggcggcgttg caagcgcata aggtttactg
     5401 ctatatagcc agcgagtgcc tttgaccgga atatttgcaa tgaaatggag atagcccatc
     5461 atcgcatcag agagcaccac gtcgcgtgcc atcccgttaa caccaggatc ggtcagtaac
     5521 tctacccatt tattaaactg cggctggaaa ccggcaatcg ccacctctgc cagctgttgc
     5581 tggaaggctt taacagcatc acggttttcc cacatgggtt gcatatcgcg tgcggcatac
     5641 aacaattgaa gctggttaag ataaaccggc ttataacctg cgggtaattg agattcgatt
     5701 tgcgtgcggg ctttttcagc cgcaccttca ggcaatggct ggatccccgc cattattgcc
     5761 gtggcttgcg cctgcggcag tgcctcgccc tgttcactga cagccaccgg gctgtcgcca
     5821 gggattactt caggctcatc ggcctgcgca ttgaacagtg gagcgaatgt tacggccagg
     5881 cacaaactga ttgccgacag ccgacgacca cacatcatat taagcaacat cccttgcccc
     5941 ctgtttttat catactctga atagccaggc tgttatttta cgacttgcct gtacaacgcc
     6001 aatactcacg aaagtatgac gagcaagcct gttactcatc aggcttatct tcagtatata
     6061 aacgccgata cgtttttaca taacctaatg taaagaagtt taaagaaaag cgcgccgtaa
     6121 tgtgccactt ttttagtgca gaaacggagt tttcggaaaa agaaaaggcg gcattgctgc
     6181 cgccttaatt ttaactcgcc tgagaaggcg cttcgtcact tcctggaagc gtttcaggga
     6241 gttgaggcgc aaatcctcgc aggccgacga cgtgaacgtg ttcggtattc tggaagactt
     6301 tacgtaccag tttataggtg gtgcctttct ccgggctgat attttccggc gctgcgataa
     6361 tgagctgcat ttgcaaacgt tcacacaatt caaacagcgt ggcgatggaa cgggcgtcca
     6421 gtcgcgctgc ttcatcgagg aacagcaggc ggcaaggaga gatatcttta ccgcgcaggc
     6481 ggcgagattc atcttcccag ctttgtacca ccatcaccag aatcgacata ccggtaccaa
     6541 tcgcctcacc ggtcgacaat gcaccagact ctgcacgcaa ccagccatcg gaaccacggt
     6601 taacctcaac ttccatttcc agatagttgc ggtaatccag cagttcttca ccgatggtct
     6661 gcggcgtgcg ctgccccata tcaatctgcg ggttaagacg ttgatacagt ttcgccagcg
     6721 cttctgagaa ggtcaggcgg ttgctgttaa acagatcctg atgctgctcg tgctgttcag
     6781 agagcacatc cagcagcatg gcgtgcgttt cacgtacgtt cacgttcaga cgtacgctgt
     6841 tcacctggcc aaacgatacg ttctgcaacc cctggttgag catacggata cggttctgct
     6901 cgcgctgaat ggttttgcga atgatgttcg ccacgctgcg ggaactgatc gccagtttct
     6961 gttcacgaga ggttaattct tcggtcagac ggctaagctc gatctccatc tgttcgatag
     7021 cttccaccgg atcatcggta cgaataatat cctgacgaat acgttcacgc agatgctgat
     7081 aaaccgccac gaagaactga attttacgtt ccggacgttt tggatcttcc gacatccgca
     7141 gcacgtcgcg cagatgttcg ttatccgcca ccgccagacg cagcgcacct aacgccttat
     7201 ccgacatgga gcgcaaatca tcagcggaga gataagccag ctcacgacgg tgtaagcgac
     7261 gctcaacgcc attatctttc accatgcgca tcaccgcaca ccagcccgct ttggcggtca
     7321 ctacctgctc gcgcatctca aagtaatccc gctccagctt gcgcagttta cgggtcaggt
     7381 tgtccatctc cgcttcgcag aaggtaagcg ctttttccag ttgattgcgt cgtgaacggt
     7441 tattgctcag ttgcgcgtgc aactcgtcac ggcgaatacg tgcccgctct tctgccccgc
     7501 tatcagcacg cacgccgata tcctgcaatt cacgttgcag atcgttaagt agctcttttt
     7561 tggtgtcata cgaacttttc agcgaagcca gcacctggtt gtactgattc aactgcgctg
     7621 cgtgtccgcg caacgcttca cgagcgcggg tacgctccgc ttccgcctgt tccagacgtt
     7681 cacgcagttt ttcgttgaga tcgctgttac cgctaagcat ttctgccgag tcagagtagc
     7741 taaagtgcgc acgacgctgc accacttccg tcagggcaaa cgcctgctga cgggcatcgc
     7801 gctgcatctg ctgagagtac gcgtaatctt cttttaattg ttcgaactgt tccgggtcgc
     7861 tctgcaatac cgaaacgatc ggctccagtt tcgccagttg attgccaaac tgctgaacaa
     7921 aacgcgcggc ttcctgggct tcatccagac gttcgcggat ttcatcgacg cgatccgcca
     7981 ggctgtcatc agccaacagg ttgagacgcg gcagaatgcg gttcagcgcc gtaacgccct
     8041 ctttcgcctg ctcaaactga atacgctgct gctggttatc attttcatga ttactcaacg
     8101 cccgttccag ttcgacgcga cggctgttca gttgacggat ttctgcttcc gggtcagact
     8161 caaacgccac cgccagatga ctgccgataa agcggctgaa cgcctgatgc agacgctggg
     8221 ttttctgtac atcaaaggaa agcgtggcga agcgttcgga aagcacttcc cgctcggcat
     8281 ggaggctttc aatacggctt tcacgcgcag cacgaccaaa cagcggcact tccgggaaac
     8341 gtgaataacg ccactgacga tcggcgattt tcaccaccac cgctttttcc agctcatcaa
     8401 cgctgaacac gctgtcatcg aatgactgcg gatccccttc gatcaaatag agatcttccg
     8461 ggcaatcggt caagccttcc aggtgttcgg ttacctgtga cagatctggc accacgatgg
     8521 cgtggcgtga cgggccatac agtgctgaga agtacggcgc atcttccagg ctaacgtcgt
     8581 cataaatttc tgacagcagc acaccaccaa aacgctccgc cagcgcgttc agacgctgat
     8641 cttcagaacc acctggctgg cttaaacgtt cgatctcttc atcgacggcg tttttgcgcg
     8701 cgcctacttc atcgcgttca acaattgcct cgcgctcacg ctccagcaac tgttgcagaa
     8761 attcggtgac atcctggctg gaggtgaact cttcgccgca ctgttcgctc aactggttga
     8821 gactgttttg cgctgccagc caaaccggcg cacgctgcat caaactctga atgcgagact
     8881 gcagctgttc ctgctcctgg cgcagtgcca tccgctcttc tcgggcgtta gagacgctat
     8941 cggaaagaga ggcaatgcgt gcttccagct cctgatgcag agcttccagt tcatcaatat
     9001 caaaattctt gccctgacgt ttgcagaaat ctgccagcag acgctcagct tcttgctgct
     9061 cacgcagacg ctgttccagt tcgcttaagc gcatccgcaa cggctgaacc tgctctgcca
     9121 ggtgacgctg atcgacccct tcgcgcaata gttcgcgagc gacatcccac gcctcgttac
     9181 gcgccagcgg gccgttgatt gccaccacca gctgataagc ctgctcaaac tggctgtgcg
     9241 cggtttgcgc catgctcatt ttctgctcaa gagagagcat tttttcggtc gcttccagct
     9301 ctttcgcctg gaaggtttcc agccattcgg cagcgctgtc ggcggttaag tccggcagat
     9361 ggcacagttc tttggcacga ttaagcgcag caatcgcctg gttatactgg atcgcgcgcg
     9421 tttgctggac gtccagcgcc tgctggtagt cagcaagctg gcttttcagc tcgtccactt
     9481 ccagttcggc agcttccgca cgagcctcgt tctcttcctg gcgttcgatg gcttctgcca
     9541 ccacttcgtt ttgctcttcc agacggatct gcagctcatc gagatccgct tcgtagcgtt
     9601 cgatcttctc ctgctgacgc agtgcggttt gcaccaggtt caggtgatcg ctggccgcct
     9661 gataatccgc ttccagatcg ccttcggcac cgttgtgctc agccagctct cgcgccatgt
     9721 cgacgtgttt gtactgctca gccgccagtt gctgacgcga agtatgtagc tcgcgacgaa
     9781 actccagggc cttatcgaga tgaacacggc gctcgttggc gtgacgcatg tagtccgccg
     9841 ccacgtagtt ggtggcttcg ctgatcagat gcttaaacag gtcacggtcc gactgggtga
     9901 cacgaatcgc ttccagcgtc atgcggtttt cacgcagcgc cgcttccatg tcctggaacg
     9961 ctttccacac cccactgttt tccggcaaca ggtagtcgcg cagagaacga gtaatggcgc
    10021 tggagatccc gccatacagc gaggcttcga tcagacgata gaatttacta cggtcagacg
    10081 ctgagcgcag acgacgcgcg atgatgccca gatcgaacat cagcgagtgg taatcggtaa
    10141 tggagttgaa ctgtttaaac tgcacgcctt ccatcgcctc gagcttgtct ttcagctcgt
    10201 taagcggcag cacgcgcgcc tggcgttcgt tcagagtttc ggtcaccagt tgtgtcggct
    10261 gcaccgacat cggcagtccc tgaatggcaa acggcttgat atccacttta cgatcgcgtc
    10321 cggcaacctg ttgcagacgc acaccgacca ccacgcgctg gtgacgcgag ttaatggtgt
    10381 cgagcatcga ataacagaca cccgctttca gcttaccgtg cagaccttta tcgcgcgaac
    10441 cgctggtggc cccggcttcc gtagtgttac ggaaatgcag cagggtcaga tcggggataa
    10501 gcgccgtaac gaacgccgcc atggtggtgg atttacccgc cccgttaccg ccagaaagcg
    10561 tcgtgaccag ctcgtcaagg tcaaaagttc gggcaaaaaa gccgttccag ttaatcagcg
    10621 tcagtgagcg aaatttaccg cgttcaatca ttattcttcc tctccgctat ctggctgact
    10681 ctcttcggtt tcatcgttga gttgcagatg attttcaatc ggcattgctt caccatcacg
    10741 aatcaggcga cgctgtgctt cacggggatc gtcgccagca cgcacatcgg cgccgaagcg
    10801 gaacaccgat tcggtaatgc ggaacttgct gctgtcgtgg cccataaacc acaccatgcc
    10861 taaacgacgc agacggttga gcgaagaacg tactttttcc tgcaacttct gacggtcaac
    10921 gtctgaaccg gttgaacggt tgttcaccag tttcagcagt ttcgcttcat cggccagggt
    10981 gagcagttcg tcgtacagtt cctgttgagt aaaaatcccc tcattcgcca gccgttccgg
    11041 gctgagatag agataacaga ggattttccc gaccatcata tccagttccg acaagacgga
    11101 acgagggatc agcgtggtgg aacgtgggcg tagatagaag aacccttccg gtgcacgaat
    11161 aagctcaacg ttataacgcg cgtaaaactc ttccagatat tcctgaaaat ccatcaggaa
    11221 tgcatgatta tccagttcgt cgaggccaat atggcggcct gaacgtaagg cgctatccag
    11281 cgccggaaat aacggattcg ccagcgcctg cgccagctta accggcatca cttgttcaat
    11341 atttgtcgat gacatgcgcc tgtaccttgg ctccgtaatc attaatcggc tgccatttcg
    11401 ctggcagtcc ggtgaaatct gcttgcgcta cgccaagacg taccgcctga tcaataacaa
    11461 tgcgcgcaac gtcaaagtga cgtgcacgcg gatactgtga cagatattcg cgtaccacca
    11521 gaccaagatc caacggcact tgtctggttt tgtacacggc aagttgttct tcgatgatcg
    11581 ccgccagctg ttcgcggatc tcgttaaact cttcgtactc cagatcctca ggaagttccc
    11641 cagtcacttc ttcatcgcgc agtaccatct cttcgtcacg catatccagc agacgatcgg
    11701 cattggcata agttagcgcc cacggctcat caaaataggt ttgtaccgac tgacgtaacc
    11761 gctgagcaaa gacgcggttt ttatccatat cgatcgcagt acgaataaat ttgtgtacgt
    11821 ggcggtcgta gccaatccac aagtcgatgg attgctggcc ccaactgata atacggtcga
    11881 gtttgctctg aagatcgaac accagacgat cgacaaaatg caggtcgtca tgggtcatcg
    11941 tcgcatcctg aatgcgtaac aggttggcct gcaatttatc gcctgccgct tccagcgtat
    12001 cctgcaattc acgcagcgtt ccggaagttt ctgaaagcaa caattcacag ctggaaatcg
    12061 ccgcccgcca gtctttgttc aacaactggg cgatatcatc cttcacctgc tgctgctgtt
    12121 catccatcag acgttgcgtc aggtcgatac tgtcgaaaat ttctgctacc gaatatttca
    12181 gtggcgcata aacattgcgg tgccagtgaa attcatcacc gccctcttcg gcggcatctg
    12241 ctgcgcgttt gagctcaccc gccacaatcg acaattgcat agaaaggcgc agcgtagaaa
    12301 actcgcgctg acgaatgtaa tagtcagtga tgccgatgcc gagcggcgtc agacggtaaa
    12361 ttgcgttccc ttccgcctgc tcgctggtaa agcggttcag cagacgttga cgcaccatgt
    12421 cgttgatcgc gttgttggcg cgcacgccga tggtttcgct ggtttgctca aacgcatcac
    12481 tcacatggcg gaatgcatcc accagctcgc cttcactcat ctcaccatcc agacgctcgc
    12541 cgttcagcgt ggcaaccgcc agcagaaaag agagtcggtc taccggcagc gagatggaga
    12601 agtcattttt tctggcccag gcaaccagtt cggggactgt ctgggaaaat tcactcatac
    12661 tttatccttg ctctgcggtt tgcgcgcggt gacatgaata taacgcccca gggtaatata
    12721 cggttcctga cggcaataac gcgtttctaa ttcaagtaat gcttcatagc agtcgcgctg
    12781 ctggtgcttc tcacgcagat aatcatgaaa cacgcgaacg cctgtcttac ccagaatttg
    12841 ccaaccagct tcttccagcc acagataaac ctgcgccggg tcgcgtggat aatctggcga
    12901 aagcgtccgt tttttctttt tcggcattcc cgcctgcacg taatcaaaat tcccggcgac
    12961 catgttatgc atcaacaaac catgcgcatt gtagaacatt aacgacaaca cgccgcctgg
    13021 acgcaacact gaccacaggg tctgtaatac gctgcgggga tcggccaccc actcgagcac
    13081 cgcatggaac aatatcagat caacgggcgt ttccaaatgc gaagcaacat cctgagcggc
    13141 gcaatgtata aattgcatgt tgtcgctcac accttttgct tctgccgcct gttttgcgcg
    13201 gtcgatcatc tgcgcagaaa gatcgcataa aatgacctga tgcccgcgct cggccatttt
    13261 gattgcggtc tgcccttctc caccgccagc atccagcaca cgcagttttt gcgggcctat
    13321 ttctgccagc acgcgatcga gatcctgcca cagaatagcc tgtcgaagct gccctttggt
    13381 ggtgccgtaa atgttacggg aaaacttttc cgcaatatca tcaaaattgc gatcctgcat
    13441 tgctttctcc accggttggc taagacgttt tctgataaag acgctatttt gtcacagcca
    13501 cggcgagaat gaacccgtct ggtgtaatat cgctggcgat cccttgctat atggttaaaa
    13561 aaggaaccag aaaggatgct ttttacactg aaaaaagtga ttggcaatat gttgctgcca
    13621 cttccgctaa tgttgttgat tattggcgct ggcctggcgc tgctgtggtt tagccgtttt
    13681 caaaaaaccg ggaagatttt tattagcatt gggtggctgg cgctcttgtt attgagtttg
    13741 caaccggtag ccgaccgcct gctgcgtccc atcgaaagca cctatccgac atggaataat
    13801 tcgcaaaaag tggactatat cgtggtgctc ggaggtggtt atacctggaa cccgcagtgg
    13861 gcgccgagct ctaatttaat caataacagt ctgccacgcc tgaatgaagg tattcgctta
    13921 tggcgcgaaa atccgggatc aaaactgatc ttcacgggag gcgtagctaa aaccaatacg
    13981 gtgagcacag ccgaagtcgg tgccagagtt gcgcaatcgc tgggcgtgcc gcgcgagcaa
    14041 attatcaccc tggatttgcc aaaagatacc gaagaagaag ctgcagcagt gaaacaggcg
    14101 attggtgacg cacctttcct gctggtgacc tccgcatcac atctgccgcg ggcgatgatc
    14161 ttttttcagc aggaagggtt aaatccgcta ccagccccgg cgaatcagtt ggccatcgac
    14221 tcgccgctta atccgtggga acgggcaatc ccttctccgg tttggttgat gcatagcgac
    14281 cgcgttggct atgaaacgct tggccggatc tggcagtggc tgaaaggttc gtcaggcgag
    14341 ccacggcaag agtgattttg atgccagatc gaagttacgc cgattaaatc gtccggtgtt
    14401 gaccagttgc gcaacttcat cccataacac atacaaccaa cgccgccaga tgaacgactc
    14461 cgccactggc gcgcgttgca gataactcca gagcaaatct tccgccagag aattatccat
    14521 tagtcggaac agttcgtatt ctcgcggtgc ccaaagcatt agtcccggtc cgaccatcgc
    14581 cagtaactga tcgctgcgcg aatctttcag catgctgcgt aaacagaagt taccgtgaat
    14641 cagtacacaa ttgtcgttaa agccttcaaa taatgccggg agacattcgc gagtgcgaaa
    14701 caggatccgc ttatcctgca tcgatagacc ggtgttattg aactgattga gcgtggtcca
    14761 tagcacttca acatgttgcc ggtaccatga gggccagaaa ttttcctgag tattgtcgac
    14821 cgcgccgacg caaccacgac tgtcctgacg gtgccaggcc agtaaggctt caacgatttg
    14881 gtctttgagt tgttcccagc gttctggtgt tcgggctggt gcctccaccg aaacaccacg
    14941 cattcgctcc agtagcagga catcggggcc ggggtgttct tcatgcgtca tcacaccata
    15001 aatagtcggc atacggacag tcccacttct tgccagcatg gtggttttcc atgccagttg
    15061 tcgggctttt ccgggcgtac taaagcttct tgccattaac ggcattgggt ttccctggct
    15121 gtcatacaaa gcccacaacg ccgtatccgc tttttcattg acgcactcaa tacgactgag
    15181 tttttcgccc agtaaatggc ttaattcggc acgcagctgt tccattcaga ttaccctcat
    15241 aaaagctact gctttcataa tgagcgccaa tataaaagat gtcacctgaa tgagatcaat
    15301 tgttgaaaag aaaaattgag gaaaatcgta acggtacgac actcctccca aaattggctg
    15361 gagtgtcgtg aagtgaaatt agcgcatttc agcgcgaacg cgctcgagat cttcaggggt
    15421 atccacacct gtgccaggaa cttcctgggc gacagcaacg tggatttttt cgccatacca
    15481 cagaacacga agctgctcta acatttcgat atgttctaac ggacttggct gccaggtaac
    15541 ataacgacgg ataaagcctg ctcggtagcc ataaatacca agatgacgca ggaagttatc
    15601 gccaacggtt tcaaggtctt ttgcaaaacg atcacgatcc cacggaatgg tggcgcgaga
    15661 gaagtacagt gcgtaccctt cggcatcgag aaccactttc accgcattcg ggttaaacgc
    15721 ttcttccgca ttgtggattg gcaccgccag agtcgccata cccacctgac gctgagcgag
    15781 gttatcagca acctgacgaa tgattgtcgc agggatcatc ggttcatcac cctgcacatt
    15841 aacgatcacc gtgtcgtcgc tgaatgcgca tttttcgaca acttccgcca aacgttctgt
    15901 acctgactga tgatcggcgc gcgtcataca tacttcgccg ccagcggctt caacagcgcg
    15961 ggcaacatcc tcatgatcgg ttgccacgat gatgcgctcg gcacctgatt cgcgcgcgcg
    16021 ttcaagaaca tgaacaatca tgggtttgcc gttaatatca accaatggtt taccgggcag
    16081 acgcgtggac gcgtaacgcg cgggaataat gaccacaaaa ctcatgattt accctcatca
    16141 gcagtcagca cgcgggcttc cgtttccagc aacactggaa tgccatcgcg cagcgggaaa
    16201 gccaggttgt ccagtttgca aataagctct tgtttttcct ggttatacca aagttttccg
    16261 ttgcaaaccg ggcaggcaat gatttcaagc agacgatgat ccatagttcc tccagatgga
    16321 tcgggttatg aatgcataaa tcttatcata atcatttatg cgacggggtc tatttcccag
    16381 ccggcgcggc aatcagtaaa gaggccttgc gagcagcgtc ctaatgtcac ccgcgttgcc
    16441 tgctgccagt tagcgaaatc agtaatcgcc tgacgtaacc ctttttgcag cgttgtcgtt
    16501 ggtttaatgc cttcctgtaa ccacagagag ataacttcaa ggatgccttt ctggcgatgc
    16561 attttggaat ccattcgccc aactaattgc ccacgatgta ataacggcag aacaaaatag
    16621 ccatactggc gtttcggcgc tggggtatag cactccagcc ggtagctaaa atcaaaaagc
    16681 tgctccgcgc gtttgcgatc ccagacaaca ggatcaaaag gcgaaagtac cgcgctgtgc
    16741 gttgcagtga gctttccggc gagcgcacgc tcgagtagcg gcagcaaatc agcatgcagc
    16801 caaagattgc ccaatttttc aacatgcaca gcaatgattt gctgctgttc agccctcgct
    16861 tcacgccacg ccgccagtgc cggacgtttt agccggtagt aatctgccag ccactgttcg
    16921 cgaaatattc ccaggctacg cgcactgtta tccagcatga tgatttctgc ttctgcttgc
    16981 gaaacgagat cgcgctcatc atcccagtca ggcatgacac ggtgggttaa atcataaacg
    17041 cgctggaagt tgcgccgttc aatcaccatc acctttccgg cagtaaataa accttccaga
    17101 tgtcgtttat gcggcttcca ctcccaccag ccgcttgcac ctttacgagg atgctcaaaa
    17161 tcggctgaac gtaccggccc cctatcatga atatgctgaa ttaacagtgc aatttccgcc
    17221 gcatgttcct gcatccaggc gtctttatat ttccagccca ttttttcagg tgccagcatg
    17281 cggtggcgaa taagacgaaa atcgctacgc ggcataaagc aggcttcatg cgcccagtat
    17341 tccattaatt cgccacgcgc cagagactca tccagccact gggcaggata atttcccaga
    17401 cgactgaaaa gcaccagata tgggctacgg gcaacaatat tgatggtatc gatttgcagc
    17461 aaggacatgc gggagatcgt tgccggaata tcctccaacg acgctcgacg gtggggtttg
    17521 tttaacaggc cttgtgcagc gagatgaaga ttacgcgcat cagcaaggga gaggtgcggc
    17581 agagacattc atgactccat caatcgaacg ctgccgcggc gtaactagtt gccagaagcc
    17641 agcgaagtta gttgcgcaag cagtttcgct ggttcatcac ctgaaagctg tgcgtctaca
    17701 ggcaaatacc accaattttc ttctgcaaag gcccggcatt tcaccgcatc tttttcagtc
    17761 attaccagcg tttgtccggc gcttaccaac gcgctgacat ccgcatgatt caaaaactga
    17821 tgatcggcca gcggtacaca tttttcgggt tgtacgccac acatcttcag cgtggcaaaa
    17881 aagcgcggcg gatgcccaat ccccgcaatc gccaccacat gttcaagctg agcaacgtca
    17941 caacgtgtac cggtacgtaa attcaccgcc tgacccggca gcagatgcat ggggatttca
    18001 ccgctgcggg ggacaccgcc gttgacgatt accgcatcaa ccaactttaa gcgccctgct
    18061 cgctcacgca ttggccccgc cggcaaccac cagccattgc caaagcgacg cacaccatca
    18121 ataacgacaa tttccacatc acgcgccaga cgataatgct gtaaaccatc gtcagttacg
    18181 atgatctgca catcagggtg ttgcgccaga atggctttta ccgcgtcaga acgcacggga
    18241 gaaaccgcaa caggcgcgcc agtgcgttga taaatcaaca caggctcatc acccgcctgt
    18301 gctgtggtgg tatctgccga caataacagc ggataagatt cagccttacc accatatccc
    18361 cgcgatacga cccccacgcg aataccgcgc tgttgcagct gttccaccag ccagacaacg
    18421 accggggttt ttccgttgcc gcctgcagtg agatttccaa ccacgacaac cggtacgggg
    18481 gcacgccagg cgcgcttcag ttttagttta tagcaaagac ggatcgcgcc actcaccagg
    18541 ccatacaacc aggagagtgg cagcaatagc cgccataaag gggactcacc agaccagatt
    18601 ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgcca cggtgctcaa
    18661 gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacaacc acgatttcgt
    18721 ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg tttttctgca
    18781 actcatccag tgctgcctga atcgcacgtt cggattcggt atccaacgcc gaggtagctt
    18841 cgtccagaat cagtatcggg ctatcacgca acaaggctcg agcgatagca atacgctgac
    18901 gctgaccgcc agagagcaac acgccgtttt caccaatgac tgtatcgaga ccgttatcca
    18961 tcttattgat gaagtccatg gcgtaggcca tacgcgcagc ttcttcaatt tgctcacggc
    19021 tgtactgttc agtccttgcg taagcaatgt tgttggcaac cgtatcgtta aacagatgga
    19081 cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac tcgcgcagat
    19141 cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgtgtg attaggctgg
    19201 cgatggttga tttacctgaa ccagagcggc caaccagagc aaccgtcttc cctgccggaa
    19261 ttttcaggtt gatgttacgc aatgcaggca catcacgtcc cggataagta aaggtgacat
    19321 tgcgaaactc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg tctttctcct
    19381 gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc tggaactggg
    19441 cgttaacgtt ggtcagcgat ttcagcggac gcataagtgc aatcattgaa gagaaaacaa
    19501 cggtaatcgt accggcagtc aggctatcca tgacacttgg gaagcttgcc gcatacagaa
    19561 caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg gaagaggctg
    19621 aaaccatttt catcccctga agacgcattc ggttgctgac tttatcgaag cgtttcgttt
    19681 ccacttcctg accaccgaaa atcaatactt ctttatggcc tttcagcatt tgttcggcgc
    19741 tggtggtcac ctgccccatg gtgttctgca tgtttttact gatgttgcga aaacgcttcg
    19801 atacaacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg atcgacagtt
    19861 gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct tcacgcacaa
    19921 cagtaatcag tgcgccggaa gaagaagaag cgacctgttc ggaatcgtag gtgatacgtg
    19981 acaacagcgt acccgttgac tgtttgtcaa agaatgaaac cggcattccc atcatgtgac
    20041 caaacaggcg gcgacgcata gtcattacca cctttcctga tacccaggag atacagtagc
    20101 tggagatata gctggtgata ccacgtaaaa tcatcagccc gatcaccacc agcggcatcc
    20161 acatcagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc ttaaggagcg
    20221 ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc acgatcagac
    20281 ccgctttaaa aggcgcaatg gttggccaca gtcggcggaa cgtctgccac gtagagagat
    20341 ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata ttctacccgt
    20401 tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc tctctgaggc
    20461 tgctaatcca ccagccttgc gctgaaaaat tgaccgttat ttgcccctga tgtggagtat
    20521 caagccactt ataaccctgt tgatgatagc gatatttaac tttgcttgag ggcagtcgcc
    20581 atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc tgaattaatg
    20641 gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagtaatgtt gcctgcattt
    20701 gctgccagta acggcttagc atcttttgtt cagcgggggc ttcaatatca ccggttagaa
    20761 gaatgctatt cgtcccgtca tcaatcttaa ccacacagga atggttattt cctttatcgt
    20821 tgctaccttg taaaggccag tgcgcgctga aacgcaatcc ttgccattgc cacgcttcgc
    20881 cacgcacaca gggctgatga tgttcccagt ttaacggact tcggatccat aacatcggcc
    20941 atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga ctcaaaataa
    21001 cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt tgcccactat
    21061 ccccttcggg ccaggccagt cccgtgtcat agaggatcgc tttgccgttt cgcgcaatga
    21121 ccattgccag cccctgcccg acatcaagca tgtacacttg ccactcgtca ggtcgaggtt
    21181 tttgccacag cggcaagcac atcagcaagc ctacagccac acacattgct ggcaacgttc
    21241 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgtt agccattgcc
    21301 aacgttcagc aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    21361 ccaaagaccg gtcggcaaga aaccataacc cttgttccag aataaacggc ccgcttaaat
    21421 gcacaaccat cgcggcgaga atcaacggaa ccgtgataaa tgtcaccagg ggaattgcaa
    21481 aaagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacaatt tgcacgggca
    21541 taagcaggag ggtgattccc agttgcagat ggatgaggga aacaaccgcg cgcaataccg
    21601 gcggcagctg ccactccgga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    21661 cagcggcaga aagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    21721 ccgccagaca acatatccat acatcccagc cactccattg tcgtccactt aacttaagca
    21781 ttccccaaat ggcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtaagcc
    21841 aggcataaaa agcagcacag cagattccac caattaatgg catttgccag tggagccagc
    21901 acccaggcag aaaaacttgc ccaccgcgaa tgagtcctgc agccagcaac gccgcaaaag
    21961 cgatgtgcaa tcctgatatc gccattaaat gcgccgttcc agtatcacgc atgatatttt
    22021 tgatttcttt ggggacggat aaccgttccc ccatacctaa tccaagaata accgcgttcc
    22081 acatataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    22141 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggttgatgc tgggcaatgg
    22201 cataacgctg gctatcaaag ccgccatcat taagttgacc atgaactgca cgaactttga
    22261 gtttcattga ccatacttgt ccggcacaaa ccgcttgcgg aagatattcg ccatacagta
    22321 cgaggcctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga ccgtaatgag
    22381 tggtcatgcc gtcagtagca atgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    22441 tttctcctgc ccacaaaatt tgctttgctg ctaatatgcc ccaaacgaaa aacagcaacg
    22501 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacacag gcgaagagag
    22561 tcagaaacgc aagggttact gtccctggca attggggcaa aatcagcaac ggaaaaattc
    22621 cgcaaattat gcatacgccg actgtcgtta ttttcatgca ccctccctgt ttatagaaag
    22681 tgtgcaactt tgttgaacgg ttgtctggtg ggagttttgc gttccccgag gcacgttcag
    22741 aagaatttaa cgatagggtg aaaactgctc aaaactctgc gaggcagctt ccagttccag
    22801 attagagaaa tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt ttgagtaaaa
    22861 acttaaccgt aaatattggc gcgatcgcgc agttctttac ctggtttaaa gtgaggaacg
    22921 tattttcctt ccagttctac tttatcgcca gtcttcggat tacgtccggt acgtggtgcg
    22981 cggtagtgca aagagaaact gccgaaaccg cggatttcaa tacgctcgcc ctgcgcaaga
    23041 gtcgaggcca tatgctccag catctctttt actgcatctt caaccgtctt ggcgggaatg
    23101 tgcgattgct gggtggcaag tctttctatc aattctgact tggtcatgat tcctccggtt
    23161 ccttaaaggc aaattaagcg gctgctgttg ctacattgat taagggcggc cgtagccgcc
    23221 cttagtgctt gattacagga cgaaacctgc aatctgtcaa gtaaactcaa caaacttcgg
    23281 aataaaaatc ccgaagagtc agagaattac tcgcctttag ctgctttgaa agcttcagcc
    23341 attgcgttgt tggagaagtt tgcatcttcc tgtttgttaa cagttgcgat tgcatctttc
    23401 tcgtcagctt cgtctttcgc acgaacagac aggctgattg cgcggttttt acgatcaacg
    23461 ccggtgaatt tagcttcaac ttcgtcgcca acgctcagaa ccagggtagc gtcttcaacg
    23521 cggtcacggg atgcttcaga agcacgcagg taaccttcaa cgccgtcagc cagttctacg
    23581 gttgcgcctt tagcgtcaac tgcagttact ttaccggtta cgatagcgcc tttcttgttc
    23641 agagcaaccc agttgttgaa cggatcttct gcgagctgtt taacgcccag ggagatacgt
    23701 tcacgttctg cgtcaacctg cagaacaact gcagcgattt cgtcgccttt tttgtattca
    23761 cgaactgctt cttcgcctgc aacgttccag gagatgtcag acaggtgaac caggccgtcg
    23821 atgccgccgt ccaggccgat gaagataccg aagtcagtga tagacttgat tttaccttca
    23881 acacggtcgc ccttgttgtg ggtttccgcg aactgctgcc acgggttagc tttgcactgt
    23941 ttcagaccca gggagatacg acgacgttct tcgtcgatat ccagaaccat aacttccact
    24001 acatcgccaa cgttaacaac tttggacggg tggatgtttt tgttggtcca atccatttcg
    24061 gaaacgtgta ccaggccttc aacgccttct tcgatttcaa cgaagcagcc gtagtcggtc
    24121 aggttggtca cgcgaccagt cagtttggta ccttccggat aacgtttagc gatagctacc
    24181 cacggatctt cgcccagctg tttcaggccc agggatacac gggtacgttc gcggtcgaac
    24241 ttcagcactt taacagtgat ttcgtcgccc acgttgacga tttcgctcgg atgcttaacg
    24301 cgtttccagg ccatgtcagt gatgtgcagc aggccgtcaa cgccgcccag atcaacgaat
    24361 gcaccgtagt cagtgaggtt cttaacgata cctttaactt ccatgccttc ctgcaggttt
    24421 tccagcagct gatcgcgctc tgcgctgttt tcggattcga taacggcacg acgagaaaca
    24481 acaacgttgt tgcgcttctg atccagcttg attactttga attcaagctc tttgccttcc
    24541 aggtgcagag tgtcacgcac cggacgaaca tctaccagag aacctggcag gaacgcacga
    24601 ataccgttca gctcaacagt gaagccgccc ttaactttgc cgttgataac accggtaaca
    24661 gtttcagcat cttcgtaagc tttttccagc gtgatccagg cttcgtgacg tttagctttc
    24721 tcacgggaca gcagagtttc accgaagccg tcttctactg cgtccagagc aacgtcaact
    24781 tcgtcaccta cctggatttc cagctcgccc tgggcgtttt tgaactgctc agccgggatg
    24841 gcggactcag atttcagacc agcgtcaacc agtactacgt ctttgtcgat agcaacaaca
    24901 acgccacgaa cgatagaacc cgggcgggtt tcgatttctt ttaaggactc ttcaaagagt
    24961 tgagcaaaag attcagtcat gtttaatctt caggtttata tttaacgtcc acctggctcc
    25021 atgccggatg gggttgttta acatacccgc tgtcattcca ttgcaacggg ggtactgcaa
    25081 attcggtcgc ttatgcgaga gccaatttct ggcgcgcgta ttgtagcgct ttttcaatca
    25141 cttgctcaat gcttaaggtg gtggaatcca acactaaagc atcggctgcc ggaaccagtg
    25201 gcgctaccgc tcgattacga tcgcggtcgt cacgttcttt gatctcggcc aaaaggcgct
    25261 caaagttaac actaaagccc ttctcctgca actgtagcat gcggcgatgc gcacgttctt
    25321 ccgaggaggc gtcaaggaaa attttcactg gcgcatcagg gaataccacc gttcccatgt
    25381 cgcgcccatc ggcaatcagg cctggtaatt cgcgaaacgc gcgttggcga cgcaataatg
    25441 cttcacgaac gcgtgggaat gccgcgactt gtgaagcagc attcgccacc tcctgagtac
    25501 gaatttcgcc gctgacatct tccccttcga ggatcacttc cagattgccg ttggttgaca
    25561 caaaacgtac atccagatgg gatgccagcg gcaccagcgc atcttccgat gcaacatcaa
    25621 catggtgatg taatgccgct agtgccagta cgcgataaat tgcacccgaa tccagcagat
    25681 gccattgcaa cgcttccgcc atagccttac acaaggtgcc tttccctgca ccgcttgggc
    25741 catcaatggt aataaccggg gcaattgccg tcatctttat ctccttaaaa caggcgtacc
    25801 gttaacataa ccgcgcgcat tatacgcgcc gacgccacaa atcgttaacg ttgtgtgcaa
    25861 aaaccagttt tgtctgtatt cagatgggga tttattgcgc aattatagcg ggctggcaga
    25921 agaccagccc gaaagatgac aatgatttac ttaccagagg cgatacgatc acggatgtgt
    25981 tgcgcacgct ctgttgatgg tgggtgagaa tcaaacatgg actgggtgtg accgccatcc
    26041 aggctagcga gtttttcaaa gctaccaacc agcccctggg tactgatgcc acgtttcttc
    26101 aacagatcaa aggagaaatc atctgcttct gactctttat cacgggagta tttagcattg
    26161 atagcgcctt ctgcgatatc acccaattgt gagcgggaaa gctgggaagc cacaccgctg
    26221 gtagctgaaa tggcatcgcg tgcggccacg atcgcataag aagctttcat ttcagccagc
    26281 gagtgaccca acgcgacgtg gcccagttca tggcccagaa cgccttcaat ttcgttgtcg
    26341 ttcatcatgt ccatcaggcc actgtagaca cgaacacaac cgttcgccat cgcccatgcg
    26401 ttgacgtcgc tggtcatata aaccttatag ttgactggcg tgccattaat attgttaccc
    26461 aatgctttgg cgatttttgc cagacgtttc ccgtacttgc tggatttgct tgccacttta
    26521 ttgccgctgt ccatttcgac acagccctga ttggcgattg ctttagcatc agcatcgctt
    26581 aatgttgcgg ctttataagc attcagacct gagctgatag ccaaatcggt attaataccg
    26641 tgcgtattct gacatcctgt cagtaaggct gcagaggtcg caatcgccag caataattta
    26701 gtattcttct gattagactt gagataaatg acagggaata agtgagatat ttatgggaag
    26761 aactgggtca aaatgggaag agactaggag gaatatgcaa taaccttaca gtatgtgaaa
    26821 atcagaccgg cccgaaaaaa aaacagaact cgcgccagcc tgattgcaga tattactctt
    26881 ttttctccgt atctgcatcg ggttcatcag caaatagctt gccctgcatc cgatccagtt
    26941 cttcttttct tacccgctta accacgctgt agacccactg aagcgaaaca ccaaatttgc
    27001 gggccagttc gtggtggttg cgtccgttaa actccctgaa gatttcccgg tcgcgctgac
    27061 tgaccttcca taccatgccc atcgggaaat aaacgttttg cccgccccag acctgcatca
    27121 ttcggttcgc gacggcctga ccaatctggt cggcaacttc gggctcaata tcaataatct
    27181 cgcgaacggt ctcagaggta tgctgtgcca gttccaccag gagttccggc cccttacttc
    27241 gaaactgatt caggtcgctc atgtttgact cccgcagctc tgcgctgcca cttcttcagt
    27301 ttctcaataa cactgcttgc ctgttcagta ctgagccagc gcagggcgct gatgcccgtt
    27361 tcccgcttga tccacctcgc taatgcattt tctgaacggt cacgaacaat gccggcagca
    27421 gccatttcaa gccatagcgc acgaattttc ctggactgcg gatggttatc aagcggtaaa
    27481 ccggagctgg cttttctggc tggtttaacg cgaaagcctt tctttttcat ggattccagc
    27541 acgcagttta gttgtgtggt atccattcct ttggttgagg ctttaccggt cagcccctgt
    27601 aacatctggc ggtaggtgtc ttcatccata cccagttcat tacgggcaat atggataagc
    27661 tgtatcaggc gttttttctg catatcatct cctttttttc agtctgtcgg caacaatgtc
    27721 ggttgcagct ggcagggtga ctggccagaa caacatgacg gcggtcatgt acaggatgta
    27781 atgcgcggtg tcgtagtgcc tgccatagcc cagggaacga tgaagtcttg cgctgcatga
    27841 tcctgcgtac atataccaga aaagaaaaca gacaatggtt tcagtagtca ttctgaatac
    27901 ctccccattc gatatgaata ttacgggcag caatgacagg gtcgttattc caccatgcac
    27961 ctgacatgta tttttcaacc tgttcgcgtc cggcaataac accaattgtg atccccggcc
    28021 tgacgttctt aaaaaaggcg cgggcaaaaa ggtatctggc agatattcgg caggctttta
    28081 atttccggct tttacctgat agcgtaatca tctggcctcc agtttctgtt gttcctgccc
    28141 actgaccggg cggtgcagtc tgacgttctg cccttcacga aaccccgcat agcgcgaggc
    28201 gtcgccattg cggcttcttc ccggtttacg cgccctggtg gtttgcgtct gcgggtattt
    28261 atgttccagc cactgctgca tcagttcacg ctcatcatcg gtcagggcaa aggactgtat
    28321 ttcactgata acggccagca cccagccttc ggcaaactgg tctccacggc tggtgcgggt
    28381 ggcggttttt attcttttgt tctgtgcact gatatactgc tgacgcgcct ttttcagctg
    28441 acgggccagc acttcccagg tgtatgcagc cagtgccgca cgttcccggt tgccgtagaa
    28501 ccccacagac ggatgcgtgc cggggtgaat gatggagtta acaccaaata cctcgcggat
    28561 gatgttcatc aggcccagca tgtagcgcgg tggacggaga ctgcctgtcg gccagtagtg
    28621 actgatggtt tcatcaatat cactcatagc aatgtcggaa tgtgtgatgc cgtgaacatc
    28681 catcagttta cgggctcggc gcagtgccag agcggcctcg tgcgggttgc cggatgcggc
    28741 cagcgccagc aactttttca gtttctcaat gtgtttattc tggtctgtca ttgttctgta
    28801 tctccggtat ttttctgccg tttccatgcc cggacagcgt cggacagctc tttcaggctg
    28861 tatgctgctt taagcttttc ccataaccac tgtgttgtta tgtgcatcag cagggcagcc
    28921 agggctgccc ccgcactgag gcatgtcgcc aggccagtaa gaatcagtgt ccatgcggtg
    28981 atttccctga gtatgtcagc cattgaatgc ctcccggtta ctcgttcgtg taaatcacgc
    29041 ccagtcgtgc agccagacgt tccagttttt tctgtttgtg gaagtcaatc agccggtcca
    29101 tcccctgaag gcgcagttgc tctgtcatga tttccacgtc tgccagctct gccgcgaggt
    29161 cactttcgct gccctgtccg ttcaggttgc gggcggcact ggccgccagt tcagcggcct
    29221 cttctgccag tttcagggcc tgtgcgtccg gcccgaaacg ctgcagggcc agacggtaga
    29281 gggcggtgcg ggtgagttcg gtgtttcgtg tcatgccgcg ccctcagtgc ttccggctga
    29341 cggtgatgtg caggccgcct tctgcagtgg tttccatccg gtacggcacc tcatgctctg
    29401 ccgtgtgggt gagtgtgttc atcagtacct gcagggcagc ctcctttccg ttggtcgcca
    29461 caatggcctg tgtggccatg ctgattagcg ccgtcaggac gtgcttcaca tcggtgaggt
    29521 cgcggcattc acactcgttg acatagtgtt caacaagggt tcgggtgcgc tgtcgtgctt
    29581 cctgtggggt aatcattgcg agtcctccct gtcagggcgg gagaattcca tgacgggcac
    29641 gtctgccgaa aaatgctggc tgcagtacgg gcagaccagg gtgacgcgta ccgcagggat
    29701 gtggtattta ccggacatca cggcgatggc gctgtgaaaa cgcagggctg ttatatccct
    29761 ctcgcactga atacatttaa atatcatgat ttaattctcc tctgtttccg gcgtgcagaa
    29821 gcccgcggcg ctgacgtcgg aataaaaaag aaaatgtttt tattaaataa ttaacgtggt
    29881 gtgtttactg cacatcctgc tcaaaaggaa ttattgaaaa atcctcaatg tcgcttttaa
    29941 tggaaatacc gggaatattt ttcacggcct ctttttcatt caggatagcg tctttattta
    30001 tttcctcttt tacacgaata aagcgctcaa gccccagacg tctcagtaat tcaataacat
    30061 tatccgcccc acggatactg actgatggcg gacggtttcg ccactgcacc tcgccggtgg
    30121 tgaggttagc gaacttcacc ttcccgttgc cggtcagttc atcacggtgt gcctcacacc
    30181 atgtctgaat accggactgc agttcggcca tgcgtttttt caggctctcg gtgagcgggg
    30241 cataacgtgc ggtgatatcg ccaatggcgt cattcatttc tgtttcagcc ctgaccagtt
    30301 cacgttgtgc gtcaccgagt agtctgattc cctcaatgac ctcttcacgt gtccccggca
    30361 cccagagtgc tgctgcggcc ttgatacgtt ttgcaccttt tgtacttttt gccatttttt
    30421 atgatttctc cagttgtgct gattaccaca aagattccgg ccacacgacg cgacagccgt
    30481 gcagttcgaa aacgccctga cggaaatatc ccctgtggtc atgtccggta tacagataac
    30541 aggcctttcc ctgctcaagc atgcgcatgc aatgcgcact ccgggaaacg cggatgacag
    30601 gtttgttacc cctgatggtg atgctttgta catccgtgtt cgtcgcctta agcgccataa
    30661 tggctgactg cactttgctg atttgctggt tgatacctgt ggtggatttc attattaaac
    30721 ccctttgaca acgtcagcgt tgacctgtgg aaccccgatt tcagcggcca gattcatggc
    30781 ggctattacc aggttactga cggccagcgg atacagcagg ctgaccatat ttttacggtg
    30841 acttcccgga ttgctcaggc gggcacgtat ggcatccact gcaccggcgt ccataatgtc
    30901 cgtcagctgt ttaccggccc gttgcagttt gaacgtcaga aattcttcaa ggttattgtc
    30961 cagaggcaga agttcgacca cctcacagcg ctgaacgact tcacggactt ccatattgcg
    31021 ttcagacagt tttgtcgcca gttccggctg gccaatcagc acgatggaca gcagtttttt
    31081 gaaaccggac tccagctcaa aaaagcgttt gaggtgtttc agtgtcggaa tgggcagact
    31141 gtgggcctcc tcaatcacca gaacgtggct gaaccccgcc tggctgctgt ctttcaggac
    31201 gcgatgcaac tggcgaaaac gggcgtcctg actgcgtctg atgctttcca gtggtgcgat
    31261 ggtactgata atggcttcgg caatagctgc tgccttcagg gttttccctt tcacatcgtt
    31321 gtcttccata gcgatgatgt atggctcgat aacaattacc ggcgcattct cgcggttgat
    31381 acgttcagtc aggtcgcggc gcagcgtgga tttacccgca ccggactcac cgatgacggc
    31441 cataaaccca ccatgacggg ctgtctggta cagcgcctca cgcacgtagc gaatgtccgg
    31501 ggtggtgaac acatcatcag aaccctgcat ggcttcgtcg gcgaacgggt cacggaaaag
    31561 accaaacgct tttttggttg ctggaaataa cacctgtttt ttgagtaaca tattctcttc
    31621 ctcactgagg ctcgttttat ctgtggtacc cgctgtacgg ggcgtggccg cgccctgtac
    31681 agcatcaaaa ctcttcgttg tatcaatccc ctgactttcc agccaggacg caagacgccg
    31741 gcgcacttct ccggggctgg tgcggggcca cgcgttatga ttcacaatct gggccagcgt
    31801 ggcctcagaa acatcgacag ctctcgccac caccgcctgt ggaatacggg cctctttcag
    31861 ttgctgcttc agtaccagca tgtttccctc ctcagttgcc gttaacaatg ctgataacac
    31921 tgctgcgggc cggtgtggtc agcgtggcca tgacttcatc cagtgcggct tccggtacgc
    31981 cgtccgggta ctgtgccgtt aactggcggt aatgttccgg cgtccaggta aggccgtcgg
    32041 cgctgaactt atcgcgcagg gctttcgcgg cctccacatg agtcatggga cgttgttcag
    32101 tgcgcggccc gcgtacgtca gaggcctgac cacgcttcgg catataggcc ggaagtgtgg
    32161 tgtcgtcgat atgtttatac gggtcaagcc gcccgccgaa cggcagcgcc ttcgccttgc
    32221 gtgcggcggc tgcatctgcg gcgttgtctg taccggtaac cagcgcttcg gtttcttttg
    32281 ccgccatctg tgccggggtt tccggcaggg ctttgtaact ttcgccaaat accgccgcgc
    32341 cttcagcaaa gccaaactcg ttctttctga cctcttcgac caggaagaac gtctcgtggc
    32401 cgtcctcacc ggtcagaacc acctgtgcca catcgctgcg ccatgggtta cgggtaatca
    32461 tcagtttttc accaaccagt acgcccggta ccgttgatac gtcaaattca gtgccccgga
    32521 acgagacacg aagttttggc gtgactttgc ggagttctgg tgccgccaca gccagttcac
    32581 gacatacctc aacggaaggc gcttttttca gctgctcagc agtaatcttc agccagatat
    32641 ccgtgcgggt tttaccgtgg cggctgtgaa cagccgtggc gttaaagtgg ctgcgccatt
    32701 tcgcggccag cgcgttgagt tcttccagac tgtgaaccgg ccggaacttc agacccggct
    32761 ccagcttgcg ttcgataatg tcacgcgcct tttccacctg tccggtggcg cgggcgttat
    32821 gcggcttgtg cgctatcagg tcgatgccca gtgagcggca catgtttttc gtcatacccg
    32881 cggtgtttgc cgagccgggg tcgagataga gtattttcgg cacgccgtgc agcacgtctg
    32941 cgccgccacg ctcctgcatg gcgttgataa gcacagaaca caggttctca cctgattccg
    33001 cgcccgtcac atactcaacg taaatccagc cgctggcatg gtcggtaatc tcgtaactcc
    33061 acacgcggtc actggcgata cgggcaaggt tagcgggctt gttcttgtag aacttcgcgc
    33121 tgtccatcac ctgcagccct ttatgtccat tgctcaggta gtaaagcgtg cagagtgagg
    33181 cgtcaatctg ccagacatga ttgggatgca gactggccac ttcggtatgt ggtgcagggg
    33241 catccagttg ttccgggtgc aggccatagt tacgcagagc acggctgatg gcatcttcag
    33301 acaacgggaa aaactcactg gtggtttcat ctgttctgcc tgcggtgata aagccgttag
    33361 cccgcagggt ttccactgca tctgcgatgg aatagagacg cttaccgttc ttgcgggtgg
    33421 cctcacgcag tgtggcagat atcagcgcgg cttcgtcgcg ggtcagggcg ctgcgcccgg
    33481 catcggcgcg ttttttgcgt ttatcagtca cagatacctc cttcagcctg cgcagcagag
    33541 tggcgcggga catgccaagt tcagcacagg cagcgtcgta tattgcaccg cgtttaccat
    33601 gccccgcgtc acgtgccgcg cgggctgttt caaccagttt ttcagtcagt gccggggtca
    33661 tagcttgtct tccctgttat ccgattctgg cggcaatttt tctggcgagg tcgcacagtg
    33721 tttcgccatc gcaggttttc accacggtat ttaccggttg cggtgcaact accgggcgta
    33781 atgctgactg attctgcaga cgtcttatct gctgcatcag catcacctcc atcgtcagta
    33841 caaacgggtg cgtctcctgt gcgggtacgc agatttcacc ggaaggggtc accacgcgga
    33901 aaccctgcac catctccggc agcagattac acagtgcgtg ccccagtgcc tccgggctgt
    33961 ccatcagggt attttgtgtg ttattcgtat tcattactgc atatcctccg gtcggttgat
    34021 ttcaggttcc ggttcggtca gccaggcggg ggcatcgtta cccgatggag cggcctcaag
    34081 gtcaaacgtc tcacgtaatg catgaacact gcgttcaatc tggcagacca gaccggccat
    34141 gaaatctctg ggggtatcga tcatgttttt ggtgcagtat tcacacaggg tctcaaaaac
    34201 actggacagc cggacggcga tagccgattc ggcctcaacc gctaatgccg tcacttccgc
    34261 ccgcaatttt ttcagttctt cgtctggcgg cggaggttgc aggcgcgatt tcttctccag
    34321 ttttgtggcc agttcgttga ttttttcgtc ctttccggca agcacctgtt gctgagctgc
    34381 gttggtttcg cgcgcttcac gcagggcggc cttcagttcg cggctggtca tgcgatcaat
    34441 atcatcaagc gttgcaccgg cgacggttcc accatcagct aatgcgtcga gttcttcgtc
    34501 atccagaacc atcagttcat ataacttggt ttttcccaaa acgctcagcg ctgagcgttt
    34561 tggctgttca tctccattgc cgagaaattt cacgctggcc tgcatcatgc gacgagctac
    34621 ctgtggtgct aatccgaggt cgttttccag aatatttgtg aaatcaccat gcggttcatt
    34681 ttcttttaag atgatcagcc gcttacccgc ttccaacata gattcagcac tctgcgccat
    34741 ataaaaacgt gcttcgtgaa cgatgcgttc acgttcataa ggcagtccat caccaaactg
    34801 ctgcatgatc tccatgcgat gctgtgtcat ggcattcagg ctgacgttga gatcatccga
    34861 cagcggtaca tcggcgttca gttcagtgtt aatcggtgat ttggtacgtc ccattgatta
    34921 ctccttacaa acgactacct gaaatgacac gttggttaat ttcgttaata cgatcctgtg
    34981 cacgtgccat ctcgttactg tgggccatgg cgatctgcag taactggatg cccggtgcaa
    35041 aacgtccgtt ctccagtttc agagccagcc cttcttcaat gagggtgttg agtgcccggt
    35101 tgatgtttgc cggtgactcg tgcagtgctg tcgccagttc accgttagaa ataccgttca
    35161 gggcatgacc gcgcagcgct ttgagtaccc ggaggatgcg actgccggag cttgaaacat
    35221 tcgtcttact catctgcata cctcttttgc gatatgtgat aaccttttac aaagactcaa
    35281 aatatttagg ccgcctgagc tgttgatttc agtcccaact tcacggcgat ttcatgggat
    35341 ttaccgtaac gggctttggt ctgcccgttg agaacgcggt atacctcatt gcggttgtag
    35401 ccgttttctt ctgcccagcg ggtgaaagtg atcccgcgct ggcggaagag agctttgact
    35461 tgttctgcag tcatcgttgt ctcctttgtt gatgcaatga tgtttgcctt atatgtgtta
    35521 gattattgct cataattatg agcatatcaa gatagagatt cacttttatg agcaaagata
    35581 taggagagcg cgttatttct gtgaggaaga aagcagggtt gaaccagaga gaatttgcag
    35641 ctcgtttagg tatttctaat ggtggtatca gtcagattga aaatggaaag gcgatgcctg
    35701 gaggggagtt tctgttaaag atgcatcaag agtttaatgt tgatgtaaat tggttgctca
    35761 ctggaatgag cacagatggt atgactagaa accagaatca aatttcccca gaaaaacaaa
    35821 aactaataga tacttttgac gacatgacac cagaccaaca acgctcattt cttgagattg
    35881 ggcgattcta tactcagcca aaaccaggaa aaatcgcagg ctgatactga cccggacaca
    35941 aaggacaggt ggttgatctg gatgctttca gaaaacatcg ccagtgacat agtggaagtt
    36001 gtttggttta ccggaaagca ttgtgctttc cggttttgtt tcagaagttg cagattagca
    36061 gttcacggcg cggcgtgggt ttacccgcca gactgtagtt gatattcact gtctgtatgt
    36121 tcagtccagt gaacacctcg cgcatctgcg ggatgtcgtt caccgaaatt atcatttttc
    36181 ctctgatgca ccgggccagc tctgccagcc gcgagtagtt cccttcagga aaatccacac
    36241 catagccttc cgtacccagg tacggtgggt cgcagtagaa cagcgtgtgc ggacgatcat
    36301 aacgctcaat gcactgttgc cagtccatgt gttctatgac tgttctggaa agccgcaggt
    36361 gtgccgctga cagttcttct tcaatacgta gcaggttgaa gcgtggcggg gatgtggtgg
    36421 aggtaccgaa gctgtgctct gccaccttgc cgccaaacgc ctgcttctga aggtagtaga
    36481 accgtgccgc acgctggata tccgtcagtg tttcttccgg ggtgatctgc agccatttgt
    36541 agatctgacg gctgaccagc gcccatttga actggcggac aaattcttcc aggtgatgct
    36601 ttaccacccg gtacagattc accagttcac cgttgatatc attgatgact tcgatcttgc
    36661 cgggtgtctt aagaaaatag agcgctgctg ccccacaaaa cggctccaca taacattcat
    36721 gcgccggaaa cagcggcaga atgtgttttg caaggcggcg tttgccacca atccacggca
    36781 cgatgggtaa tgtctgcatt ttcataatct gcaagccttt tacaattatt aaaaaatatg
    36841 gcagtctggt caggtctcgc gagactgact gaaccttggt cggctcacag cgcattcctg
    36901 tggggcgatg accagcccgg ctttcgggtg ccgggctggt cgttctttca tgatgcggca
    36961 gggatggcgg gatttctgtt aatgtggttt agggattatc cggggcgatt ttctgacctt
    37021 atccggtcca gggcataagt aataccttct gcaaaaacag aagtatcaaa ctgcttttgt
    37081 attccctgaa aatgaaaagc ggcacgacag acactcagtt ctccgcggta taaccggata
    37141 atatcttcca gaacattgtt cactgcttca gcacggcgct gatgttctgt taacgttaat
    37201 cggggattca tgatgcactc cttaaatgat gatttttatg tactcaccgg caaaatattc
    37261 tgccgattac tgaacacgca tccggcacct caggttttga tttattctgg ttttttcgat
    37321 atcccggacc aggttgatca cgaccagaaa aagtgccggt gttttattga tacgctgata
    37381 tggcttcggg ataacagcta tattcgttac tctcagttgc tggctgtggg agcggctgat
    37441 gtcgtcctca cggaaaaaac gctctgtctg ctcaatgcca tgccgacctt tctgcagccc
    37501 cggcccgtac catttaaaga gtcacttctg gatgcagtaa aaaccggtga aaatgcagtc
    37561 atcgctgaat gcgtatcccg gattatgcgt tacgtgttta atgaacgtaa gtgaggttta
    37621 tccgctgtct gcataatccc gatagccagt gcgaggggaa gtaattccgg ggaaactctt
    37681 tcaaacagcg atgcccattc tcctctcgcc atcactctgt ccagcccgcc ctccagttgc
    37741 atttcagcaa tcttcctgat ttcccggaac aacgccgctt tgatctcttc atgctcagct
    37801 ttattttccg gtgtcagaag gtattcgtcg aagaaccggg gaggctggtt aatcgggatg
    37861 tgttcctgtt tgccgtactt cggggacgaa caacattctg ttgactgacc agtgagttta
    37921 cgtaactcct tcatgagcag ctcctgatcg taaactgtca tcagtgccgc aatggtggca
    37981 tgcagtgcat cgggcagtat ccagggttgc cggcccgtgg ggcatataac atccagcagc
    38041 tcattcgtcc gacagatacg ctcctcgccg tatgacaaca ggccctgtcc cgtgtattcc
    38101 tccagcctga tcactgtgcc agcccgtggt ttctgccatg tgctcagatc atccccactc
    38161 atacggcgct caccgtccac gtctgcggcg tcgacaatga cgctgtttcc gaattcctgt
    38221 ttcaccactt cctctattcg cgccagtacg taacttttac cgctgcccac aggaccggac
    38281 acggtgacag tgatgaccgg attcagtgtc tgcgtcatat cgtattctcc tctgtgaata
    38341 attcatcgtt tatcctgcac atcccgtatc cccggctctc ttaacgcgct ttaaaatcct
    38401 tcgcgccctg tatttgtgat gctgtctcca ccagataagg agacacacat gaaaaacctg
    38461 aaaaaattca ttccccctgt taaaaaacct cgcctcagcg gctggctgct gacctcagtg
    38521 ctgttgctgg gcaccatcgc tctggtctca ccacagcagc tgcctgttgt gatctacaag
    38581 ctggcactca tcacgctggc agcagtgctg ggttactggc ttgaccgttc gctcttcccc
    38641 aaagcccgtc ccggtcagta cctgaaacat gacgacaggc tgatggctga agggcgtttc
    38701 ccggtacaga ccggccttca cctggtgttt tctgctgcgt taatccgccg tgcactgatt
    38761 gttgcagcgg tctgcctggc tgttgcgatg gggctttaat catgaactgg ccgcagatca
    38821 cctggattgt atgcatgtcc ctgaaactgg ctttcgaagc ctttcgggtc ttcatcagaa
    38881 ccgaacgcct gtcagtccgc gccgggacct ttctggttca tatggcatgg gtattctttg
    38941 tcgcaatgtt gctctggtgt ggcggctttt tcagccaggc atgcgcagca caacctccgc
    39001 aggctgcgct gcagtatcgc gatgatgtga tccgtaatgc ccggcttgaa tggggactgt
    39061 ctgcgccggt ggccgatttc gccgcgcaac tgcatcagga aagcggctgg cgacctgatg
    39121 cgatctcgcc ggctggcgct cagggactgg cgcagttcat gcctgccact gccgactgga
    39181 taagccagtt gataccgatg ctgagcagtc gtgagccgtt taatccggca tgggctatcc
    39241 gggcgctggt cagctatgac cgctggctgt ggcagcgtgt cagcgccgcc agtgactgcg
    39301 agcgtatggc catgacactg tcgggctata acggtggtct gggctgggta caacgggaca
    39361 ggcggcttgc atcacagaaa ggtctggaca gcacccgctg gttcggacat gtcgccacgg
    39421 tgaatgccgg acgcaatgcg gccagctggc gggagaaccg ccattatccg cagcgcatcc
    39481 tgcgcgaact ggcaccgcga tatctcacat ggggaggcag cagttgtgtg gcatctggtt
    39541 aaaaagctgc cgtggcgcgg cattctgctg gccattctta tcaatgcctt tctggtcggc
    39601 ctgtatgcca tgggatacag aagtggtcat gactctgcaa agcgtgacgg tgataccgcg
    39661 ctaagtcagt tgcagtcagc atttgacgcg tacaaaacgg agcaggcaac gcttgagaat
    39721 gctgcgctgc gggcctgggc cagacggtat caggagcagg tggccgccgg gcagcgggct
    39781 gaagccggtt atcttgagca gattgctcaa ctggagagcc ggaacaaaca actacagggg
    39841 caaattaacg atgtcacaca gcgctggatt gatgaaaaag gtaagagtca tcccattgag
    39901 tgcgtgttta ctcgcggttt cgtgcgccag tacaacgccg cactcggata tgacaacgca
    39961 tccgtcgaca ccggtcattc agactcagtt gccgccactg gcacccgctc tggcacagcg
    40021 accgggcaac ctgaaaccac tgacacccgg ttacgcgatt cgggtgtctc ccagcgtgac
    40081 gttctcgcca acatcatcga caacgcatgg caatgtcgtc gctggcggaa ccagataaac
    40141 gcgctgctgg atgaacggga aggattacag aaatgacact gcaggttgaa ttctggacgg
    40201 tggtgagttt tctgctcacc ttcatgaggt ttgtgggagg gctcgccaaa tggttgttca
    40261 gtaaaacaga agaacgccag gcggcacgat tcgcctccct tgaacaggcc ctgcaacaat
    40321 ccgcctccaa ctggggcgag ctggaaaaag aatttatgcg atttaaagcg gatttaccgc
    40381 tgaattatgt ccgtcgagag gattatatcc gtggccagac agtcattgag gccaaactgg
    40441 acgcgcttta taacaaactg gaagtggtac agcagtaccg caataccgga ggtcaataat
    40501 ggtcgatatt acccgggtac gccgcgaatc cctgcgctgg agtctgctgg ttgccctgaa
    40561 caagacccgc ccttacaccg ccagcgagac gctgctgctg gatgtgtccc gtgccatcta
    40621 cccggacacc acaccgctgg aactgcgccg cgaactggat tatctggctg accgtaaaat
    40681 ggttgagctg gagaaaaaac cttctggcga ctggtttgct gacctgaccc gcctcggcgt
    40741 agacctggtg gaatacaccg tggaatgtgg tccgggtatt gcccgcccgg aaaagtactg
    40801 gagtgaataa tggccagacg cagcacaata gaaaagctgc cggaagacgt gcgccgctgg
    40861 cttgaacggg cgctgactga atccggcttc agcgggtata acgagctgga gtcactgttg
    40921 cgtgagcggg ggtacgtcat cagcaaatcc gctatccatc gctatggaca gaagattgag
    40981 cgccgctatg gtgctatccg tgcggcaaca gaagcggccc gcatgctgac cgaaggcgca
    41041 gcagacgatc aggatgcgcg ttcggaggct gtgatagccc ttattcagac cgagctgttc
    41101 gagagtattg tccagttgca ggaggcggaa gaaggcgaag tcgatcctaa agaacgcgtg
    41161 gccctgctgt cgaaggtggc gaagaatgtg gctacgctgt cccgcgcgtc cgtcaacctc
    41221 aaaaagttcc agtctgaagt acgggccaga gcgcagcagg cagccagcaa cgccgagaaa
    41281 attgcccgta aggggggact gtcaagcgac gcagtacagg cgcttcgtcg cgagattctg
    41341 gggattgcca catgacaaaa tcatccggag tgattttaaa cgccgctggc ggcggccccg
    41401 aagggatgag tcccatggac gggaggaata accttgctcc cgttttgcct gatacctcgg
    41461 cgctggatgc ccctcccgtt ctgttgcctt accagcagcg ctgggtggca gacacctctc
    41521 cgcttaaggt gatagaaaag agccgtcgta ccggtattac atgggctgag gcatccgata
    41581 acgtactgac cgccgcctct tctgcgccag caggcgggat gaatgtgtat tacatcgctt
    41641 ataaccagga catgaccgtc gaatacattc aggcgtgtgc gatgtgggca cgggcattca
    41701 actatgcggc cagtgaaatt gaagaaggat tctgggaaga ggacgacgac gacaaacaca
    41761 tcaagactta caccatcaaa tttcctgact ccggctttcg tattgttgcg ctctccagcc
    41821 gcccgtctaa cctgcgtggc cgtcagggta ttattgttat cgacgaagcg gcgttccatg
    41881 agcaactgga cgaactgctg aaagcggcgc tggcgatgct tatctggggg ggaaaggtac
    41941 gcgttatctc cacccatgac ggtgacgaca atccgttcaa tacgcttatc ggggatatcc
    42001 gtgccggacg tcagggaggc agcatacatc gcatcacttt ccgggaagcc gtatctgagg
    42061 ggctgttccg gcgcgtctgt ctgcgcaccg ggaaggaatg gtcggaggca tccgagcagg
    42121 cctggatggc atcggtgtac aaattctacg gtgccggcgc atccgaagag cttgactgta
    42181 ttccggccaa cggtggcggt gcctggctgt cccgtgccct gatagagtcc cgcatgtccg
    42241 ctgatacgcc ggtattgcgt ctgacctgca aggaaggtta tgaactgctg tctgatgagg
    42301 ttcgcttccg cgagacgcag gactggcttg atgagtatct gaaaccattg ctggaggcac
    42361 tccccactga tgcccgctct ttcctggggc gcgactttgg ccgtagcggt gatttgtcgg
    42421 tggactatcc cctgctgcag gagaagaacc tggtacgacg cgtgccattc gtactggagc
    42481 tgcgtaacgt gccgttcaga cagcaggagc aaatcacctg gtatctgatg gatggcctgc
    42541 ccggtctgct gggtgcagcg tttgatgccc gtggtaatgg tgcctatctg gctgaatacg
    42601 ccatgcagcg ctacggctcc ggccgggttc agcaggtgat gccaaccgaa ggctggtacc
    42661 gggagcatat gcctccggtc aaagctgcac tggaagacgg caacctggtg gacttaccaa
    42721 aggatgaaga cacactggat gacctgcggg ccgttcaggt ggtgaacggt gtcccccgcg
    42781 taccggagca acgctcaaaa gcaaaggctg acggtggtaa acgccacggg gattcagcca
    42841 tcgcactggc gctggcgtat ttcgccagcc gtgaaattaa caaagggccg gtgaaggcaa
    42901 gctcacgccg tcgtcgtcag gcggcccgta tgctggaggg attctgatgg cgaggggtat
    42961 ctgggtttca cccgatgaat ttgttgcttt ttctgagcct cagaaatcac tgaccgcgca
    43021 gattgcctcc cgcagccgcg cgatcgactt ttacggactg ggcatgtatc tgcccaatcc
    43081 tgatcccatt ctcaaggctc agggacggga tatccgtatc taccgcgaac tgcgcaccga
    43141 cccgctggtc gggggctgta tccgcagacg taaagcagcg ctcaaatcgc tggagcgtgg
    43201 actggagcgt ggtcacgcct ctgcccgggt cttccgtttc atccgcgaca tgctcgacga
    43261 tctggatctg tcccgcatca tcggtgaaat gagcgatgcc gtgctctacg ggtatcagcc
    43321 ctgtgaaatc atgtggggcc gttcggtcag gtcgtgggcg gtgacggata ttgtcggcaa
    43381 accgcctgag tggtttcagt ttgatacgga caactgcctg cgctttcggg cgcgtgatgc
    43441 gggtgtggag ggtgagctgc tgtcaccgtc aaaattcgtg gtgccggcac aggatgcctc
    43501 gtatgacaat ccttacggtt tcccggacct gtccatgtgc ttctggccgg tcgccttcaa
    43561 gaaaggcggg atgaaattct ggctccgctt tgccgaaaag tttggctccc cgtgggtgat
    43621 cggtaagcac ccgaggggcg caaatgatgc agagattgaa aaactgctgg actccatgga
    43681 gcagatggtg gaggacgcgg tggccgccat ccccgatgac agcagtattg agctcagggc
    43741 agcggatggc aaggcagaca gcagcgaggt atttcgcgag ctgatcacac tgtcacgcag
    43801 tgagatctcc attgcattac tcggtcagaa tcagaccacg gaagcgaaca gtaacaaggc
    43861 ctctgcacag gccgggctgg aggtaacggc tgatatccgc gatgcggatg cggacatcat
    43921 tcaggcggca gtgaatcagg tcatcagaac ggtggtcacc ctgaacttcg gcgatgtgcc
    43981 gtgtccggtc tgggccatgt gggaacagga ggccattgat gacacccgtg ccacccgcga
    44041 cgaaaaactc acccgggcgg gtctgcgtct gaccccgcag tacttcaagc gtgagtacca
    44101 actgcaggac ggcgatattg acgagacacc accgtcggaa cgccagaata acatgctgcc
    44161 gctgtcattt gccgaggcga ttgatgctga tattcaggct cagcagcagc ttgacgacgc
    44221 gctggacatt ctgatgaacg gaggcgcgtt aaatggcacg ctggaacccg tcctggcacc
    44281 tctgttcagg cgggtcgaaa acggggttaa cccgtctgag ctgctgggcg aactggcgga
    44341 gctctaccct cagatgaaca cggacgatct gcaggagcgg ctggcccgca ttctctttgt
    44401 ggcaaatatc cggggacgtc tgcatgagcg tgacaacggc tgaactggcg tactgcatga
    44461 cgcttccccc gaagcgggca atcaactacc tgaagtccaa aggatatcag attacctggg
    44521 actgggaaga aatgtggcag gaggcccatg cccgcgcctt taccgttgct aaagtgaccc
    44581 gcctggatat cctggaagat attcgcgggg cactgcagca ggctgtcgat gaaggaaaaa
    44641 ccgatcgctg gttccggcag gagctggagc cggtgctgaa gcgtaaggga tggtggggac
    44701 cacgtgacac gactgacccg gtaacgggtg agccggtcac cattcagcag ggcagtccgt
    44761 ggcggctcga taccatcttt cgcaccaata tgtccgtact ctacagcgcc ggtcgttggg
    44821 cggaacagat ggaaaacgtc gacgacaggc cgtactggat gtataccggc atcaacgaca
    44881 gccatacccg caggagccat ctggcgctgc atggtctggt gctgcgctgg gatgacccgt
    44941 tctggcaggc attttacccg ccgaacggct ggcgctgccg ctgtagtgtt attgccctga
    45001 gtgcggcgga tgtacgtgcc cgtggcctga aggttatcag ctccggctct gccatgggcc
    45061 aggaactgaa actggtctca gagaaaaccg gcgaaatgcg gaacgtggcc acctttaata
    45121 ccggcaccac gaaggtgacc accgacgtcg gctggtctta tgctccgggg gcagcatacc
    45181 gtcccgacct ggcccgctat cagggtacgc ttcagccact ggcacaacag gaactgagag
    45241 gataacaatg gcttccgata acctggtcag tatcaccatt aacgataagt ccctgcgccg
    45301 gagcctccgt gcgctggatc ttgctgccac agacctggag cccgcgatgc gcaaaatcgc
    45361 cggaaccctg ctggcggaaa cacagtttaa ctttcttgat gaggggcgtc cggggtggat
    45421 gccctcgctg gcagcggaag aacgtgacgg gcagacactg caggataccg ggcgtctgat
    45481 ggggtcagta tcaaccgacc atgacgaccg gcaggctgtt gtggggacca acgttgttta
    45541 cggtgccatt caccagttcg ggggtaaaac ggggcgtaat gagtctgttg aacttccggc
    45601 ccgcccgttc ctgccggtga cgggggatgg agaactacag cctgaagtgg taatccccat
    45661 cctcgatacc attgtccgcc atcttgaatc agcggcccgt cgctgagttt tctctcttca
    45721 ggcgggtgat ttatcattgc cagcgaatga ggggctgtat tacctttata aaggctttac
    45781 agcctctgtt ttataaccgc ctccggttca ccgcattgct ttccctgtcc ttctcccctg
    45841 atgttttcta aagcagatta aaatcgccgg gcctgcattt ctcacaaact gtctccgaca
    45901 acataacgcg ggacagcaaa atgtcagcca ttcacatttt taaagccggt actcataccg
    45961 atatgcacgg caaaaaactg ccgttcacgc cagacgatct tgccgcctgc gtgaaagcct
    46021 atgacccgtc cgtccatgaa gcaccactcg tgattggtca tcccagaacg gaagacccgg
    46081 cgtggggctg ggtgaaagcc ctgtcgctca gcggcgtcga tctgatggca gagcctgccc
    46141 agctggaccc gcagtttgct gagatggtca ccgacggacg attcaaaaaa gtgtccgcct
    46201 ctttctacct cccggattca ccgtccaatc cgaagcccgg cgtgctctac cttcgccatg
    46261 tgggctttct cggggcacag ccaccttccg tcaaggggct gaaacaggtg tccttcagtg
    46321 agcaggaaga aggtgtggtg gagttcgccg actggcaggc catcacgaat gcctccctgt
    46381 ggggaaagct gcgcgatttt ctgatcgccc gcttcagtct ggacgaggca gaaaaagtcc
    46441 tgccggaatg gcagctcaac agtctgcgcg aagaggcgta ccgcgacaca ctgtcgcagg
    46501 atgcagcagg tgcacaattc agtgagacag gcccggggcc gtcttccgca agtaacgagg
    46561 aatcatcgat gacaaaagaa gagattgaag cccttcagga ggagaaccgc cgcctgaagc
    46621 agcaggctgc tgatcgcgat gcgcgtgatg cacaggtcag acaggagcaa ctgcataagg
    46681 acaatgtggc ctttgcagaa aaactggtcg cagagggccg tctggctccc cgcgcctcct
    46741 ccgtggtggt tgccctgctg gatgccgtcg ccggtggcga caagccggtg gagtttgctg
    46801 agggggaaag ccgcacaccg ctggccaccg cctttcgttc attgctctcc gacggggagc
    46861 cggtgatgaa tttcgccgaa caggccacaa aagagcgtgt cggcgacacg gtgaaggtgg
    46921 atgtggcaga gtttgcggaa gccgatcctg agcgtctggc cctgcatcag aaagcagtgg
    46981 ccctgtccaa aaaagaaggc atcagctatg aggctgctgt cgcacgctgc ctgtaattta
    47041 aggagagagc atgtctgatt acttaaaagg taaacgtgtc gttgatccgg tactgaccag
    47101 tatcgcccgt ggctataaaa atgccgcatt catcggcgaa cgtattttcc ccgtcgtgct
    47161 gacggacaag gaaggcgtgc gtgtaccgac cttcgggaaa accgcctttg tggaatatga
    47221 caccgagcgt gccgtcgggg cggacagcaa tgttctggtc cgtgaaaaaa caggcacgct
    47281 ggacctggtg ctgggtgaac acgatctggc tgcgccggtg gactatcgcg agcaggcgga
    47341 gtccatgttt aacgaagaga gcaaggccat ccgtcgcgcc acgaatggcg tgaacctgcg
    47401 ccgtgaactt atcgctgccc gtctggctca ggatgaaaag gtctaccgta ccgggcacgt
    47461 caaaaaactg acagccagtg atcgctgggc cggtggtaag ggggacccca tcggggtgat
    47521 tgaagccggt atggaagcgg tccgtacggc cacggggctg cgtcctaacc tgatgaccat
    47581 gggggccggc gtgatggcgc tgctgaagtt ccacccggcg attcaggccg ccatcggagc
    47641 caacgaacgc aagcgcatca ccacagaaat cctgcaggac ctctttcaga tcgaagagat
    47701 cgtcatcggt gccccagtct ccctgccgtc catgaaagcg gcaatggata agaacagcgt
    47761 gccggcggat atctggggag acaatctgat gctgcactat gtcggcaaac cgcagccggg
    47821 ggcggacagc gcggacgaga acgagccgtc cttcggctac accctgcgtc gtaaggggat
    47881 gcctgttgcc gacaaatacg acggagccgg tggcaaggtg aagtactgcc gttataccga
    47941 tatctacaaa gtcgccgtgg ttggtggcga tgccgggtat ctcattaccg gtatcagtaa
    48001 ataaggaggc gttatgggaa ccactcagca ggtcattctg atcacaaccg taacggcagg
    48061 ggcagcactg gcacagcagc gttttgtcgg ggcagataat accccctgta aagccggtgc
    48121 cgcagcgctc ggggttgccg aagtggatgc tgttaccggc gacagcacgc cggtgagcgt
    48181 tctgggcatt attgctgtcg aggccggggc cgctgtcagc cgtggtgtgg ctgttcagtc
    48241 agatgctcag gccagagccg tgccgcagtc cggcgacggt aaatcctgtg gtattgcact
    48301 tgatgaagcc gggggtgaag gcgacgtcat tcgtatcctg cgcggggtgt gacatgtact
    48361 gcaccctgga ggatttgctt gcgcaggtgc cggagcggac gcttatcgag ctcaccagtg
    48421 aagagatgga cttcgactca cctgcaacag tgaatacccg tgtggtggac agctgtatcc
    48481 gctatgccga cgagctgatt gatgcccatc tgcgcggacg ctatatcctg ccactggcag
    48541 agataccgac tgttctgcgg gacattgcca tcacgctggt ccgttaccgg ctctacgccc
    48601 gccgcccgga aggtgacctc ccggatacgg tgaaggatga ccacaaagaa gcgctgcggc
    48661 aactcaggga gttacgtgat aacaggctca cgctggggct gccgtccact cagaaagatg
    48721 tgcctgagcc tggcgagttt cgtgtacgca gtcgcccggc cactttcggc ggtcgtgacg
    48781 gtttactgga gaaatactga tgaacgttct gcccgtcctt gatgcggtac tggcccggtt
    48841 acgcgagaag ctgccgcaac tgcaggtgga gtacttcccg gagaaaccgg ctgaatatcg
    48901 cctgaaccat ccggttggcg cgttgctgtt gagctatgcc ggttcgcgct ttgacaggcc
    48961 ggatgatacc ggtgcggtga tccagtctca gactatccag ctctgcgtca cggtggtctt
    49021 ccgccagctc aacggtaaaa aaggggcgat taatgtcctg gatgctgtcc gccgcattct
    49081 cggtggccac accccgcccg gctgccgccg ccgtatctgg ctgacccgcg aggtgtttat
    49141 cggtgaagtc agggggctgt ggcagtacgc cctcgacttc gcgactgaaa gcgtctttat
    49201 cgaagacagc gatttaccgt ccggcccgct gttaaccgaa gtgaactatg aggaaagcga
    49261 gtgatgaaag aataccgcta ttccggcccg gccagcggcg tcacgctgtc ggacggaacc
    49321 gaaatcctgc tctggccggg gaagacggtt tccctgccgg aggagcatga ctacgtgaag
    49381 gtactggtgg cgctgaaaca tctgacgccg gtacctgaag agactaaacc cgccggcaca
    49441 ccggctgtgc agtcaccaaa gcgcagaagc agcagtgaca gcgaagtgaa aacggaggac
    49501 acccatggca gctaactatc tgcatggtcc cgaaaccatt gaggtggaaa acggtgcccg
    49561 cccggttaaa acggtgaaat ctgccgttat tggcctgatt ggtaccgccc cgatggggga
    49621 tgtcaatacg ctggtacagt gcctgtctga gaaagacgca gcggcatttg gcagccagtt
    49681 caccggcttt accattccgc aggcgctgga tgcgatttat gaccatggtg caggcaccgt
    49741 tctggtcatt aacgtcctcg acccggcgaa acataaaacg gcgatcgagg atgaggtggt
    49801 tacctttgac aaatcgacag ggcaggccag actggcgcat ccggttgtcg ctaatgtggt
    49861 ggtgaaaaac agtgaaggca gcaccaccca cacggcgaac acggactacc gtgttgatgc
    49921 gcaggcgggt gtgctcacga acctgggcaa ggctattgag gccggtggca gcgtgaaggt
    49981 gagctatgag tacgcggacc cgtcgaaggt gactgcggcg gacatcatcg gcggggtgaa
    50041 cagcgccgga aaccgaaccg gcatgaagct gcttaacgac agcttcaacc tgtacggcta
    50101 tttcgccaaa attctgattg cgccggtgtt ctgcacccag aagagtgtcg cagttgagct
    50161 tatcgccatg gcagagaagc tgggcgcggt aacctacatt gatgcgcctg tcggtaccac
    50221 ctttgcacag gctctggcag gtcgtggccc ggaaggcacc atcaacttca ataccagctc
    50281 cgaccgcgtc cgtctgtgct atccgcatgt gaaggtatat gacccggtga caaacacaga
    50341 gcgtctggag ccgctcagcc agcgtgcagc aggtctgcgt gccagagtcg atctggacaa
    50401 gggctactgg tggtcatcct ccaatcagga gattctgggg atcaccggcg tggagcgcca
    50461 gctgtccgcg atgattgatg acccgcagag tgaggtgaac ctgcttaacg aacaggggat
    50521 caccacggta ttcagcagtt acggcagcgg ccttcgtctg tggggtaacc ggacggcagc
    50581 atggccaacg gtcacccata tgcgtaactt tgagaacgtt cgccgcaccg gtgatgtgat
    50641 caacgagtcc attcgttatt tcagccagca gtacatcgac atgccgatta ctcaggcgct
    50701 gattgatgca ctgacggagt cggtcaacgc ctacggtcgc aaaatgactg gtgatggtgc
    50761 ggtactgggc ttccgttgct ggtttgatcc ggcccgcaat ccggagacgg agctggccgc
    50821 cgggcacctg ttgctgagct acaaatatac gccaccaccg ccgctggagc gactgacgtt
    50881 tgagactgag atcacctcgg aatacctgtt aaccctgaaa gggggcaact gatgtcaaag
    50941 attgagataa accgcatcac gaatgccaac atctatctgg atggtactaa cctgctggga
    51001 cgggctgagg aagttaaact ccccgatgtc tccatgatta tgcaggaaca caaggcgctg
    51061 gggatggtgg gtaaggtgga actcccggct ggttttgaca aactggaagg cgaaatcaaa
    51121 tggaacagct tttaccgcga tgcgatgctg tctgccgcga acccgtacag gtcgctggca
    51181 ctgcagtgtc gttccagcgt ccagcgctac agctcgcagg ggctgattga cgaaatcccg
    51241 ctggtcacct tcctgacgat tatgttcaag aagaacccgc tggggacgtt caaacagcac
    51301 gagaacgccg agttctccag tagcttcacc tgcacgtata tcagacaggt actggatggt
    51361 gaagagctgc tgcaactgga ctatctggcc aacatcttcc gggtcggcgg tgttgatcaa
    51421 ctgactgact accgtatcaa tatcgggggc tgacggtgag tgttgaactg acggataaag
    51481 gaggacgatg tgcggcactg ggcatgtcaa atggtacgtg gtttaccctc cttgatattc
    51541 cgggggtgga aacccttttt aatacccgta aaaccaatga cccgattgac tgcacacgtt
    51601 caaaggcccg caaactggcg gatttgattg aagcatggga gcctcccgac cactggttct
    51661 ccggcatcgg caaatctgag ggaaagacgc ttctcatcgc tttcctgcgt aactgcaagg
    51721 ggtttcgcac ttgctgacat cacaggggct ccggcccctt cttcttaatc tcctttaata
    51781 tccgtcacgc gcttctcccg acatactgcc ctgaacttac acaggagcac aatcatgtca
    51841 cagaccccat ccgatacttt taaattgtct tatcccttca ccactgctgc aggcaccaga
    51901 attgagctgg ttgaactgaa acgcctgacg gtaaaagacc tgaagcaggt gcgcaaaatc
    51961 agcaaaaacc cggcagactg ggacgaaccg ctgattgccc gcagtactgg tcttctcccg
    52021 gaggatctcg acaatatgga tctggctgat tacctgcagt tacagaaacg atttcagctc
    52081 atcacgggga tgggtgagag caaccagggc gctgacgcag gcgcaggggc tgctggcgag
    52141 atggttccgg tttcagccgg gggagattga tgccctcgat actgacgatc tggagatgtg
    52201 gctggagcag gctgaagagc aaatcaaaag cgagtacggc gacaaatcat agtacagaca
    52261 gccgccagta gcggctgttc tgcgttatcc cctcacgtct tttcaccttc cccggaggtt
    52321 aaccactatg tcgggacagt tttcagtcgg cgttgttatc ggcgggatga ttggcagcac
    52381 attccgttct gcaatgagcg gtacccgccg tgcgcttgat tccctgagcg atacctcacg
    52441 ccgcctgcag gaacgtcaga acgctttaac ccgtgcaaca gaacgttatg gtcaactggg
    52501 ttcttcccgg atgcagcatc tcaacagcga gctgctgcgg gtaagccgca ccatggagca
    52561 aattgagcgc cagcagcgcc gtctgtcagc ggcatccgct accagtgatg cgctgaaagc
    52621 taaccgcatg gcgctgtatg gtcaggggat tgaagcgtat ggcatggcac agactgttta
    52681 tcatacggtt tcccctgccg ttcagcagtc catgtctttt caggacaaaa tgattgatat
    52741 gtcgatcacc gcaaaatatg acaataaaac gcgggatgca cttgccggac agataaaagg
    52801 ctgggcgctt aaatacaatc agtatcagga tgagctgcag gaggcgatgg gttcactcat
    52861 cagcgacaat attgataatg tgtcagatat cggttttctg atgccggata ttgcccgcgc
    52921 ggcaacggca acacgcacgt ctgctcagga ctgggcaaaa gtggccgcag tctggcaaaa
    52981 ctccctgaaa ggcgcggcca gagattttgg tgccgttcag aatattatgg cttatgccgg
    53041 tgaccagggg tcatttgaaa tcccggatca ggtcaagtgg atgcagtccc tggccccaat
    53101 gatggcgggt attgccagtg gaaaagaggc tgttgctgaa atcggggcca gtctccagat
    53161 agcaaaaatc ggtgcaggtt ccaccgacga agcagccaat aattttaaaa actttcttac
    53221 caaaattttt gcccgcgata ctcagaaaca gtttgctgat ctgggtattg atttgcaggg
    53281 atctattgcg agttataaag ctgcggggat ctctccgatt gaagggatgt tgagtgttat
    53341 agaacgttac ctcaatgcca aaagccccga agcgctggcc ggcttcaaat cagccatgaa
    53401 aataaagaat gatacggcaa gagatgaggc acttcaggct ctggcgaaaa actttggtct
    53461 gggcgatatg ttcgcggata tgcaggtcat ggcatttatc cgcccgatgc tggccaacat
    53521 ggacagatat cgagagatcc gtgccggtgc tctcagggct gcggataacg atttgcttgc
    53581 cagtgcttat gatcagcggc tgaaatctcc ccttgaagcc actaaaacac ttatggtcag
    53641 cagtcgcgat ctggcaatta cgctgggcga tcaattagct ccatctttta tttctctgac
    53701 tcaggacttg ctcccactca ttcaggggac aaaacactgg gtggcgactc acccgcaatt
    53761 tgtcagtggg gcttttaagc tcatcagtgc gctccttgcg attaagatag cgactgttgg
    53821 tctcaaactg gggctgaatc tccttatttc cccctttgtg aacgtctgga aaactgctgt
    53881 tttactccgg accaactggc atcgactgac taccgcactc ggagaaggtg gcaaattacg
    53941 ctggcttgtc actggattca gccgactgac cagcggagga ctgaaactca gcaaagttct
    54001 ggcgggcagc ctcgttcgcg gttttatgag cgctgcacgt gccgttctct ggattgggcg
    54061 agcgctgatg atgaatccca tcggtctcgt tatcaccgct gtcgcggcag cagcttacct
    54121 tatctaccgc aactgggggg cagtcagtgg ctggtttaaa cagcgctggg ctgacattca
    54181 ggaagccttt aacggcggta tcgcgggaac tggtaagctg ctgattaact ggtcgccggc
    54241 aggcctgctc tataaagcct ttgcggctgc gctgaaatat ttcggcgtta ctctgccggc
    54301 aaagttcacc gacttcggtg gccatcttat cgacggtttg attaacggta tcaaaaacaa
    54361 atgggggtcg ctcaaatcca gtgtaaccgg aatgggtgac agcatcagta gctggtttaa
    54421 acaatgctgg gctgacattc aggaagcctt taacggcggt atcgcgggaa ctggtaagct
    54481 gctgattaac tggtcgccgg caggtctgct ctataaagcc tttgcagctg cgctgaaata
    54541 tcttggtgtt gatctgccgg caaagtttac cgacttcggt ggccatcttg tcgatggtct
    54601 gattaacggt atcaaaaaca aatgggagtc gctcaaatcc agtgtaaccg gaatgggcga
    54661 cagcatcagt ggctggttca gcgaaaagct gggcattcat tcgccgagcc gcgtgtttat
    54721 gggctttggt gacaatatcg cgcagggggc cgccattggc ctgcagcgga ccactccgct
    54781 tgcagctctg gccgggcagc gaatggccag tgaactgctc cccagaatgc ccgtgagcat
    54841 tcaggggcca gaaatacggg ataacacttc aggtgttcgc ttcagtatgc cgttgcccga
    54901 tatcaatggg tttatgtcgt ctgctaaaaa tgcgatcgga gccgtaatca atagtttgtc
    54961 ttccatgccc gctattccgc tctccacccg ggcatcgatt ttaccagggc aacgtctggc
    55021 aaatgagatg acaccggatg ttccccgtat cccctcgcct gaaatcctgg ctgccggata
    55081 ttcaggccgt ggtgcagctg caaccggcgg tggaacgtct ggtggtatcc aggtcagctt
    55141 taatcctcag tttttcctca atggcaggga aaccacagcg cctgcaggac tgactggtgc
    55201 cctgaatatg agtctgcatg agctggaaaa aatgctggag cgtctgctgg ctcagaaaca
    55261 acgtaaggag taccgctgat gtttgccgta ctgggtgata ttgagtttga gctgattacc
    55321 tactgggacg gcttcgaggc cacgttcggc gtcgattatg cggagcatgc ccgcatcggg
    55381 ggtaagcctg gtctgcagtt cgtcggcgac aggctggacg aaatccagat aactctggtt
    55441 ttccatcagc attattgtgt acccgatgtg gagctggcga gactgcgaac agccatgaaa
    55501 gcccatcagg cactggcgct ggtcttcggc aacggtgact atcgcggctg gttcgtgatc
    55561 accgatgtga ctgcaaccag cgagcagact gacagtaccg gcaacgtgct ggctgtcagt
    55621 gccaccgtgt ctctccggga atacaccggt gacccgaaaa atcctctgca accaccggca
    55681 atacgcacga agctcccggg tgtcggggcg gtctccggtg ccataccttc accttcaggg
    55741 gtggcgcagt tcatccgcaa cggcgtcaac tatgcgaaac aggcgcagtc tgtactccag
    55801 accactatca gcgccgttcg ggtgacacag aaaatgaagg ataaccccgt tgtcgcactg
    55861 acccgtgtgc cggggctgat gagcggactg ggtaatatct ccggggctct ggggaaaagt
    55921 gttccggcgt ttaacgcact ctctgaatcc atgcccgatg ccatcagtct ggccagaaca
    55981 gccagcgaag cagccacgta tgtacagcag gcacagtctg cgctgagtgg tgtggacaaa
    56041 agaaatattg caggtgctct ggataccgtt tccgggcagc ttaacgccgt cggcacagca
    56101 ttcaaccgca tgtctccggg attaagtgca atggccgcca gaatactgac gaggagtgtg
    56161 tgatgtttct tgaacatgtt acccgtgacg gagagcgctg ggattcgctg gcatggcagt
    56221 actacggtga cccgctgggc tatccccgga ttattgccgc caatccgcac gtggccatca
    56281 cgccggtgct gccctccggg ctgttgttac tgatcccggt gattgaggct gaagaagccc
    56341 gtacagaaga ggatattgcc ccatggctga gataaacagc actgcgcaag tcacatcagc
    56401 gttaaccggc gtcagcgatg tgctgacacc ggtgttcact ctgtggtatc tgcagaaaaa
    56461 catcacctct gatatcgcgc cttatgtcac ccgtgtgacc tggagcgata acatcaaaaa
    56521 tgagtccgat accattgagg tggagctgga cgacaccgat ggccgctggc tggataaatg
    56581 gtatccgggc aagggtgaca cgctgacgct gaaaatgggc tatcagggcg agaagctgct
    56641 gtcctgcggt acgttctcta tagacgagat cgaagtgagt tcgcccgctt ccgttgtttc
    56701 tatccgtggg gtggccacct cggttaacag tgccctgcgg actaaaacca gtcgtggttt
    56761 tgagaacacc acgctggcag ctgttgcggg gcggattgcc agaaagcacc ggctgaaact
    56821 ggtgggcagc attgagtcca tcagaatcga ccgggtgacc cagtatgctg aaaccgacgt
    56881 gggttttctg cgccggctgg ccagcgagta tggttatgca gtgaaagtgg tcagtgacca
    56941 gctgattttt tctcatctgg ccacactgcg cagtcaggag ccggtcaggc agttaaaacc
    57001 gcaggatgtg gcccgctttt ccctgcgtga caccatcaac cgggtctata aatctgcaaa
    57061 ggtaaaacac cagaaaagca gcagtaaaaa actgatcgtc tacgaagctg atggtggtac
    57121 ccgtgaaagc gacaaaaagc tcaaaggtgg taaggttacc agcgctgact cacttaaagt
    57181 taacagccgc gtcagcgacc cggacagtgc ccggattaaa gcggattcag cactggccag
    57241 acataacgaa taccagcaga acggctccct gacgctgacg ggaacacctc aactgacagc
    57301 aggcaacaaa attgaactgg tgagttttgg gcagttatcc gggccatggc tcataaccac
    57361 tgcccgccat gcgtttgacc gtaacagcgg ctacaccaca gagctggaag tggcacgggg
    57421 gccagtcaca agagggaaaa aacaaaaaac tcagaaactc acggtttatc acccggatgg
    57481 cagtacatcg acggtgatta aggagaagaa aaaatgactg gtgtcactcg tcaggtcggt
    57541 acggtcagtg ccgttgatgc cgacagggtt caggcccgcg ttcgtctgcc tgaatgcgat
    57601 aacctgcgca caaactggct taacgtgctg cagcgcaata cccaggataa caaagattac
    57661 tggctccctg acgtggggga gcaggttgag gtgctgctcg atgccaacgg cgaggatggt
    57721 gttattctgg gcgcggtgta ttcagacgtc gataaaccac cgttcagtga caaaaatatc
    57781 cggggaaccc ggtttgctga tggtgcagag tacagctaca accggaagac gcacactctg
    57841 atcatccggg gcggcattga gcatattgtc attgagtgtg gtgctgatgt ggtattgaaa
    57901 acacagaaag ccacgattga cgcaccggaa accgaactta ccggagatct gcgtgtcagg
    57961 ggtaagctga tttacgaagg aggcatggcg ggttctggtg gtgaaggtgt taccgcgacc
    58021 atccatggca atatcgagat taaagggaat gtccatgcca cgggcagtat gttgtctgat
    58081 ggcgaaaact ccagccacca ctcccactga gctttttaaa cgcctttaat atcagcgttc
    58141 ccgcacgggg gcaatactgc ccccatgaaa acaacctcag tattctggca accagccctg
    58201 caggctcccg gcgaaattgt ccgggggctg gatgatatcc ggcaggcgat tcaaattatc
    58261 ctgcggactc cccgcggcag cgacccgcat cgcccggagt tcggcagcaa tctgcatctt
    58321 tatatcgact ggcctgtaga ccgggccatt ccgcatgtgg tgcgcgaatc cgtcgatgcc
    58381 atccggcgct gggagccccg ctgccagctt atgtcagtta aacccgccgt cgacggcgaa
    58441 catcttacgc tccgggtgag ctggaaaggc tcagacggac agacccggac tcaggagctg
    58501 ctatggcgct gacagaaccc gattttattg aacgcgatgc cgacaaaatc acggcagaaa
    58561 tgattgcgaa gtatgaagcg gataccggca aaacgctgta cccggcacag gcagaacgtc
    58621 tgctgattga tctgtgggcc tatcgcgaaa tgctggtcag ggttgcggta caggaggcag
    58681 caaagcagaa tctggtcgcc tttgcccgtg agccgatgat tgattacctc ggtgaactgg
    58741 tcggtgtata ccgtcttgcc gcgcagcctg ccaccaccac gctccagttc tccgtagatg
    58801 aggcactggc cattgatgtg ctgatcccgg caggcacccg cgtcagcgct tccgacagcg
    58861 ttatttttgc caccgataca gatgtggtac tgaaggccgg attgctgctg gtcaatgtca
    58921 cggccacctg taccgaaccc ggtaccgctg gtaacggctg gcaacctgcg caggtcagtc
    58981 agttactcga tgagattgat aacgttaacc tgctggtgag caatctgatg gccagttccg
    59041 gcggttcaga acaggaagac gatgacaggc tccgggagcg tatcaggctg gccccggagt
    59101 cattcaccaa tgccggaagc cgtggcgcat accgctttca tgccatgcag gcccatccca
    59161 acattgtcga tgttgctgtg ctttccccgg ttcccgggac cgtagatctg tatccgctgc
    59221 tcagtaccgg tctgccggac ggcggtgttc tcacgctggt agagagtttc tgctctgatg
    59281 agaaagtcag gccactcact gatacagtgc gggctaaaac accagtgaag gtggattaca
    59341 ccattgaagc caggattacg atctatcgtg atcaggatgc caggtctgta aaagatgccg
    59401 ctaacagcgc catacagaac tgggtggcat cacgtgccgc cacgctgggg cgcgatattg
    59461 tccccagcca gattatcagt gcattgtccg tttccggggt gtaccaggtt gaactggtga
    59521 caccggcact gcgggtggtg gcagaaaacg aatgggcaaa ctgtacggca atcactctta
    59581 acatgactgg agtgtctgat gactgagcca ttacaactcc cgccaccgct tgagggtgat
    59641 atcagtctca ggacgctggg aagactggca ggacggctgg ataacatcga cctgagcgta
    59701 ctgatggtct ttctcgtcga tatcgtcgac agttccgcgc tgccatggct gggcgagcag
    59761 ttctcactgt ctggcgatgg ctgggagctg gcggaatcgg acgatgttcg ccgcatgctt
    59821 ataaaagcag ccatcgaact gcaccggtat aaagggacgc cgtggtcaat ccgggaagtt
    59881 atccgccgtt ttggctttgg tgaagtggat ctgattgaag gcacaggtcg tctcagttac
    59941 gacggcaatc gcagctataa cggactgttt gttcatggag atgcagccgc ctgggcagtt
    60001 tatcgcgtta ttctgaaaca gcccattact aacgatcagg ccgcgatgct gcgtcagacg
    60061 ctggctgcat ttgcaccggc ccgctgccat ctggccagcc tggagtatca gtctgtggcc
    60121 attcgctata acaataccgc catccatgat ggcagttata accacgggag cagttgatta
    60181 tgggaaacct gaatgaaaca gaaaagtggg aagaaaatat ctatcaactg gagacatcag
    60241 atccggttct gggtggtgca gacgggatat caaatcgagc cccccggcaa ctggcaaaca
    60301 ggacgaaatg gctgaagaag aaaacggagg aagccgcaca gtcactggct gaacacgtac
    60361 gttcccgtaa ccacccggac gcgacactga cagctaaggg gttcacccaa ctgagcagtg
    60421 ccaccaacag cacctctgaa acgctggccg ccacaccaaa agcggtcaag gctgcatacg
    60481 acctggcagc cggcaaggcg cctgccagcc acactcaccc gtggagtcag ataacgggag
    60541 tgcctgcggc ttcactgacg gcaaaaggca ccgtacaact gagcagcgcc acggacagtc
    60601 aatcagaaac tgaggctgcc acgccgaagg cggtcaaggc tgcatacgac cttgcagccg
    60661 gcaaggcgcc cgtcagccac actcacccgt ggagtcagat aacgggagtg cctgctgcct
    60721 cgctgacggc aaaaggcacc gtacaattga gcagtgccat caacagcacg tctgaaatac
    60781 tggccgccac accgaaagcg gtcaaggctg catacgatct ggcaaacgga aaacaaccgg
    60841 cagatgccac gctcactgct ctggcaggac ttgccactgc agcagatcgg ttgccttatt
    60901 ttaccggagc agaccgcgca gagctggcaa cccttacagc tattggtcgc gctattatcg
    60961 ctaagggcag tataaaagat gtcctcaatt accttggttt gggggaaggc tctgcattgc
    61021 ctgttggtgt accggttcca tggcccaccg ccacaccgcc agcaggctgg ttacagtgca
    61081 acggagctac gttcaccaaa gaacaatatc ctgttctggt cagagtctac ccgaccctcc
    61141 gtcttcccga tttgcgcggt gagtttatcc gtggatggga cggtggtcgc aaggttgata
    61201 ccgggcgtgc gctactttca tttcaggaag ggactatcgt atccggtttt gatgataatg
    61261 ataccggaga tatcagttcg ctcggctcta cacaatacgg atttggtgac gctttaacct
    61321 ctaatcaact gggggtctta aatggcaaaa agtggatatt tgatgcatcc tctaaaggtg
    61381 cacaaagata tgactggtgg gcttatgtat cagctcgccc ccgcaatatc gcatttaact
    61441 atatcgtgag ggcggcgtga taccgttcag ccggcaagcg ccagtggcgc ttccggccag
    61501 ctgatttcag gtgccttgct gatatccagt gcgttcagct cgtcgatata atccagcacg
    61561 gcgttcagtt ttgccgtttc ttctggtttc agactgcgac cagcacgcag tttgaggttg
    61621 ataagatcga cggacgccat cgcctgtttc acccgttgct ggcgttgctg tctggcaacc
    61681 tccagttctg cggctcgctg ccgttcagta tcggttaccc acgcctcacc gttccaggta
    61741 tcccatggcg tggctggctg ttttgtggtg gtgtccgccg ggtaatcacc cggttgggta
    61801 atcagaacgg ggttaccgtt ctccgtgctg tataccgtct cgccgcggtg atcaggcaca
    61861 tgctcccacc cgttcagcgt ggcagtccgg caaaccacgt aacccgaaat tgcttccggc
    61921 ggtgcatcgg cgcacgaatg tgccggagta cccaccccaa gggcgagata ctccacagag
    61981 gatgaggtgt attcccgggt ttcctcgtca tagtgatata cagtgatatc gcccgctaca
    62041 gtggcgatac cgttttcatc cagaactgca gtgttactca ttaaactgcc ctcacaatgt
    62101 aattaagtgc aatgctacgg ggtctgacag tgataccctt cgcatacccg cttctgatag
    62161 aggtcagaat agtagcctgc ataccattgt ctgacattgc cacgacagga tcgctgaatg
    62221 ttgtcccgtc cggcatttta aagctgccct cacgaaggtc tgtaatggaa tcttcattgt
    62281 tgaatcccat gccgactatc cccgaggttc ccgtagtgtc ctggttataa taatccagca
    62341 ttgctgttct taacagcgtt gccccttgtg cggacagcaa cttacgtcct gtatcaactt
    62401 t
//
LOCUS       DACADA010000008        58554 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.008, whole genome shotgun sequence.
ACCESSION   DACADA010000008 DACADA010000000
VERSION     DACADA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.008"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(75..290)
                     /locus_tag="ISB17_000513"
     CDS             complement(75..290)
                     /locus_tag="ISB17_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978492.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
     gene            complement(353..1825)
                     /gene="der"
                     /locus_tag="ISB17_000514"
     CDS             complement(353..1825)
                     /gene="der"
                     /locus_tag="ISB17_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP1978493.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1943..3121)
                     /gene="bamB"
                     /locus_tag="ISB17_000515"
     CDS             complement(1943..3121)
                     /gene="bamB"
                     /locus_tag="ISB17_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAP1978494.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3132..3752)
                     /locus_tag="ISB17_000516"
     CDS             complement(3132..3752)
                     /locus_tag="ISB17_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAP1978495.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3770..5044)
                     /gene="hisS"
                     /locus_tag="ISB17_000517"
     CDS             complement(3770..5044)
                     /gene="hisS"
                     /locus_tag="ISB17_000517"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP1978496.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5155..6273)
                     /gene="ispG"
                     /locus_tag="ISB17_000518"
                     /gene_synonym="gcpE"
     CDS             complement(5155..6273)
                     /gene="ispG"
                     /locus_tag="ISB17_000518"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAP1978497.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6300..7307)
                     /gene="rodZ"
                     /locus_tag="ISB17_000519"
     CDS             complement(6300..7307)
                     /gene="rodZ"
                     /locus_tag="ISB17_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAP1978498.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7592..8746)
                     /gene="trmG/rlmN"
                     /locus_tag="ISB17_000520"
     CDS             complement(7592..8746)
                     /gene="trmG/rlmN"
                     /locus_tag="ISB17_000520"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAP1978499.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8896..9327)
                     /gene="ndk"
                     /locus_tag="ISB17_000521"
     CDS             complement(8896..9327)
                     /gene="ndk"
                     /locus_tag="ISB17_000521"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP1978500.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9476..11788)
                     /gene="pbpC"
                     /locus_tag="ISB17_000522"
     CDS             complement(9476..11788)
                     /gene="pbpC"
                     /locus_tag="ISB17_000522"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAP1978501.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11789..16750)
                     /gene="yfhM"
                     /locus_tag="ISB17_000523"
     CDS             complement(11789..16750)
                     /gene="yfhM"
                     /locus_tag="ISB17_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAP1978502.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16957..17802
                     /gene="sseA"
                     /locus_tag="ISB17_000524"
     CDS             16957..17802
                     /gene="sseA"
                     /locus_tag="ISB17_000524"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAP1978503.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18295..19071)
                     /gene="sseB"
                     /locus_tag="ISB17_000525"
     CDS             complement(18295..19071)
                     /gene="sseB"
                     /locus_tag="ISB17_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAP1978504.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19213..20496)
                     /gene="pepB"
                     /locus_tag="ISB17_000526"
     CDS             complement(19213..20496)
                     /gene="pepB"
                     /locus_tag="ISB17_000526"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAP1978505.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20555..20755)
                     /gene="iscX"
                     /locus_tag="ISB17_000527"
     CDS             complement(20555..20755)
                     /gene="iscX"
                     /locus_tag="ISB17_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAP1978506.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20767..21102)
                     /gene="fdx"
                     /locus_tag="ISB17_000528"
     CDS             complement(20767..21102)
                     /gene="fdx"
                     /locus_tag="ISB17_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAP1978507.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21104..22954)
                     /gene="hscA"
                     /locus_tag="ISB17_000529"
     CDS             complement(21104..22954)
                     /gene="hscA"
                     /locus_tag="ISB17_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAP1978508.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22971..23486)
                     /gene="hscB"
                     /locus_tag="ISB17_000530"
     CDS             complement(22971..23486)
                     /gene="hscB"
                     /locus_tag="ISB17_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAP1978509.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23582..23905)
                     /gene="iscA"
                     /locus_tag="ISB17_000531"
     CDS             complement(23582..23905)
                     /gene="iscA"
                     /locus_tag="ISB17_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAP1978510.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23922..24308)
                     /gene="iscU"
                     /locus_tag="ISB17_000532"
     CDS             complement(23922..24308)
                     /gene="iscU"
                     /locus_tag="ISB17_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAP1978511.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24336..25550)
                     /gene="iscS"
                     /locus_tag="ISB17_000533"
     CDS             complement(24336..25550)
                     /gene="iscS"
                     /locus_tag="ISB17_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP1978512.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25662..26150)
                     /gene="iscR"
                     /locus_tag="ISB17_000534"
     CDS             complement(25662..26150)
                     /gene="iscR"
                     /locus_tag="ISB17_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAP1978513.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26421..27161)
                     /gene="trmJ"
                     /locus_tag="ISB17_000535"
     CDS             complement(26421..27161)
                     /gene="trmJ"
                     /locus_tag="ISB17_000535"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAP1978514.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27280..28083
                     /gene="suhB"
                     /locus_tag="ISB17_000536"
     CDS             27280..28083
                     /gene="suhB"
                     /locus_tag="ISB17_000536"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAP1978515.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDSTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28228..29082
                     /locus_tag="ISB17_000537"
     CDS             28228..29082
                     /locus_tag="ISB17_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1978516.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29273..30553
                     /gene="csiE"
                     /locus_tag="ISB17_000538"
     CDS             29273..30553
                     /gene="csiE"
                     /locus_tag="ISB17_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAP1978517.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30545..31684)
                     /locus_tag="ISB17_000539"
     CDS             complement(30545..31684)
                     /locus_tag="ISB17_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAP1978518.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            32054..32476
                     /locus_tag="ISB17_000540"
     CDS             32054..32476
                     /locus_tag="ISB17_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP1978519.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32524..33396)
                     /locus_tag="ISB17_000541"
     CDS             complement(32524..33396)
                     /locus_tag="ISB17_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP1978520.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33408..34502)
                     /locus_tag="ISB17_000542"
     CDS             complement(33408..34502)
                     /locus_tag="ISB17_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP1978521.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34535..35533)
                     /locus_tag="ISB17_000543"
     CDS             complement(34535..35533)
                     /locus_tag="ISB17_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1978522.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFSVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35536..37065)
                     /locus_tag="ISB17_000544"
     CDS             complement(35536..37065)
                     /locus_tag="ISB17_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1978523.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(37088..38071)
                     /locus_tag="ISB17_000545"
     CDS             complement(37088..38071)
                     /locus_tag="ISB17_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP1978524.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38168..41449)
                     /locus_tag="ISB17_000546"
     CDS             complement(38168..41449)
                     /locus_tag="ISB17_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAP1978525.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41567..42760
                     /locus_tag="ISB17_000547"
     CDS             41567..42760
                     /locus_tag="ISB17_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1978526.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42824..44077)
                     /locus_tag="ISB17_000548"
     CDS             complement(42824..44077)
                     /locus_tag="ISB17_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP1978527.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44406..45596
                     /gene="hmpA"
                     /locus_tag="ISB17_000549"
     CDS             44406..45596
                     /gene="hmpA"
                     /locus_tag="ISB17_000549"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAP1978528.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45641..45979)
                     /gene="glnB"
                     /locus_tag="ISB17_000550"
     CDS             complement(45641..45979)
                     /gene="glnB"
                     /locus_tag="ISB17_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAP1978529.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46040..47374)
                     /gene="glrR"
                     /locus_tag="ISB17_000551"
     CDS             complement(46040..47374)
                     /gene="glrR"
                     /locus_tag="ISB17_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAP1978530.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRNELDANDFKE"
     gene            complement(47364..48077)
                     /gene="qseG"
                     /locus_tag="ISB17_000552"
     CDS             complement(47364..48077)
                     /gene="qseG"
                     /locus_tag="ISB17_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAP1978531.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48242..49669)
                     /gene="qseE"
                     /locus_tag="ISB17_000553"
     CDS             complement(48242..49669)
                     /gene="qseE"
                     /locus_tag="ISB17_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAP1978532.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50245..54132)
                     /gene="purL"
                     /locus_tag="ISB17_000554"
                     /gene_synonym="purI"
     CDS             complement(50245..54132)
                     /gene="purL"
                     /locus_tag="ISB17_000554"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAP1978533.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54390..55946
                     /gene="mltF"
                     /locus_tag="ISB17_000555"
     CDS             54390..55946
                     /gene="mltF"
                     /locus_tag="ISB17_000555"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAP1978534.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55943..56479)
                     /gene="tadA"
                     /locus_tag="ISB17_000556"
     CDS             complement(55943..56479)
                     /gene="tadA"
                     /locus_tag="ISB17_000556"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP1978535.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56504..57139)
                     /gene="yfhb"
                     /locus_tag="ISB17_000557"
     CDS             complement(56504..57139)
                     /gene="yfhb"
                     /locus_tag="ISB17_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAP1978536.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57348..58196
                     /locus_tag="ISB17_000558"
     CDS             57348..58196
                     /locus_tag="ISB17_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1978537.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58252..58512
                     /gene="yfhL"
                     /locus_tag="ISB17_000559"
     CDS             58252..58512
                     /gene="yfhL"
                     /locus_tag="ISB17_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAP1978538.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13857 a        16309 c        14576 g        13812 t
ORIGIN      
        1 ttattccacc ataacagatt gttttatcat ttgcacatta aatacaaatg acaaaacaat
       61 acattcaagt gatattaagc aataacaaat tcaacgcgtt ttttagatat taaaccgtgc
      121 ccatgttggc agaaataatc cacagcacca caagctttta acacttgtaa cggctgatga
      181 cactcagggc aaagtgcctg gacggtaaag tctttgtcgc aagttgcaca gtgagcagtc
      241 tcgccattgc gttcgagagg ggttttgcag gttggacagt tgagtgccat aaatactcct
      301 cgcgtgttat tcggtttccc tctccgtgtg gaaagggaaa ccagataagt gattacttac
      361 ttttcttgat gtgcttcatc agacgcttac gcttgcgcat ctgagtcggc gtcagggtat
      421 tacgcttgtt cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca
      481 ttacgtccag ggatttgcgg aagtagttca tcaggtaacg cttgtaggaa tcaggcaggt
      541 ctttcacctg attgccgtga atcaccacaa tcggcgggtt ataacccccg gcgtgggcgt
      601 atttcagctt cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg
      661 tcatgatgcg cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac
      721 gtactgattc aaacaagttt ccaacaccac tgccgtgcaa ggcagagata aagtgcacac
      781 gagcaaaatc gataaagccc agacggaagt ccagcgtctc tttcacctgc tctttcactt
      841 cctggctcag gccgtcccac ttgttcacta caatcacaag tgagcgccca ctattgagaa
      901 taaagcccag caaggagaga tcctgatcgg aaataccttc gcgcgcatca atcaccaaca
      961 tcaccacgtt ggcatcttca atggcctgca acgttttgat taccgagaat ttctctaccg
     1021 catcggtgat tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc
     1081 cgtcgcgttc cattggaatg tagatactgt cacgcgtcgt gccaggcatg tcgtaaacaa
     1141 caacgcgctc ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcgggcgac
     1201 ccacaatcgc cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt
     1261 cgccgttctc ttccgcttca aattgcgccc agtattcggc gtcttcgtcg acttcttctt
     1321 gcggtgcgag atcttccatc cacggcagca gcacgtgctc cagcagactt aatacgccac
     1381 gaccgtgaga cgcggcgatc gggtagattt cacctaaacc aagcgcgtag aaatcaacca
     1441 ctgcctgatc gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac
     1501 gggagcgcag atgtttagca atcgcttcat ccgccggcat caggcccgcg cgcgcatcca
     1561 ccataaacag tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg
     1621 tttctacacc gtcttctgtg ccatcaatcc cgccggtatc aatgcagata aactcacggc
     1681 cttcaatttc cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca
     1741 gcgcatctcg ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc
     1801 cgacaagcgc gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac
     1861 gcacgctata ttcgtgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt
     1921 tcaaagtgaa cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct
     1981 ggatcagcag tttgccgtca gcagcaaccg gttcagtctg gaaaccggaa ctatcaactt
     2041 tttgctgggc aacgaaacga ccatcttcga cgttaatcca gtgcagataa ccttcactgt
     2101 cgcctaccac caggttgcca ttatacagca ccggagaagt cagcaggcga tgcagcagat
     2161 cgctttgtgt ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt
     2221 gatcgaccag atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc
     2281 gtttccacat aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca
     2341 gcgcgaaaac aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa
     2401 tttcggtaga accggtcgcc tgggaaatac gctgctgcca aatcatctgg ccctgttcca
     2461 tcagcactgc gctgacgcga ccattatcgc cacccacgac ggccgcacca aaagccgttg
     2521 ccggcgcaga ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga
     2581 cagcgccgtc agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca
     2641 gaccgtcgct gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca
     2701 cagtaccatc gctggtattc agcgcgtaaa cctgcgcctt ttcggtgcca atgtagacat
     2761 gcccaccaga cacggtcacg ccgccagaaa gtaatgcagg atctttagag aaccagccat
     2821 ctttctcggc caggttgaca gaccagattt ctttgccgtc atccgcattc agcgctttta
     2881 ctaaaccagc gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg
     2941 aatagaagtt gccaatgccg ctaccaacgg aagtgctcca cacagtagtc ggcgtaaact
     3001 ggttttcaac ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg
     3061 aacagccgct taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca
     3121 tcgggtccct ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc
     3181 ggggtaacat cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct
     3241 ttgctcagca atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg
     3301 atagcatcaa gggtttttag cgcggcatca gcctgcttga gctgtacctg aacgcgagca
     3361 agacgcagat ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt
     3421 aactgggcgg cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa
     3481 gaagccagcg caccataagt atttttattt tcagcagcaa atttttccgc cgctgggatg
     3541 ctatccggtt tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag
     3601 cgtgcagaat caacctgatg gctattccag tagcgccagc caatcagtgc gccaacgccc
     3661 aaaatcaccc caacagccag tgctttgcca ttttcagcaa aaaagcgttt aaccgcttca
     3721 acctggtcgt tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta
     3781 acgtgcgcaa atgcgcggct acgctatcct gcgcaactgc agtttgctca ccagagcgca
     3841 aatccttcac tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc
     3901 gggcacccca tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg
     3961 tcatcagttt cacacccggt aattcatcac gcagacgctc tgctaatgcc atagccgcag
     4021 attgtgtgtc agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt
     4081 ccggattaac ggcctgaact aacaatacaa gacgttcgag gcccatcgca aaaccgactg
     4141 ccggtgttgc acgaccaccc agttgttcca ccagaccgtc ataacgacca cctgcacaca
     4201 cggtgccctg ggaaccgagg ctgttagtca cccactcgaa aacggtacgg ttatagtaat
     4261 ccagaccgcg caccagacgc tggtttacag tgtaagcgat ccccgcgctc tcaagcagtt
     4321 tgcacagacc ggcaaagtgc tcacgagatt cctcgtccag ataatcgcct aatgccggag
     4381 cgtcgttgag aagcacctgc acttccggat tttttgaatc cagtacgcgc aacgggttag
     4441 tgtacatgcg gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca
     4501 ccagcgcatc gcggtaattc gcacgagctt ccagcgaacc gatagagttc agctcaagag
     4561 ttacgtgctc ggaaataccc agcgcgcgcc accagcgggc agtgagcata atcagttcgg
     4621 cgtcgatatc cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat
     4681 aacgcccttt ctgcggacgc tcgtgacgga acataggccc gatataccac agacgctgtt
     4741 cctgattgta cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag
     4801 ggcgcagagt caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca
     4861 cgtcggtgac ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc
     4921 ggatttcact gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc
     4981 agatggccgt ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg
     5041 ccacgtttat tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttcg
     5101 ggagccatgc gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt
     5161 ttcaacctgc tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat
     5221 gcgcgcttcc agctgatcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc
     5281 ttcatagagg ccgcttttct tgttgccgcc agtgacgccg agtgtagaaa ccagcgcctc
     5341 acctgggcca ttcaccacgc agccgataat cgaaacgtcc atcggagtga ttatatcttc
     5401 caggcgttgc tccagcgcgt taaccgtacc gataacatca aattcctgac gcgaacaggt
     5461 cgggcaggcg atgaagttga tcccgcgcga acggatacgc agcgatttca aaatatcgaa
     5521 accgactttg atctcttcga ccggatcggc cgccagcgat acgcgcagcg tgtcgccgat
     5581 gccttcagac agcagcagac ctaaaccaat agcggatttt actgccccgc tgcgcgcacc
     5641 accggcttcg gtgatcccca gatgcagcgg ctgatcgatt tgttttgcca gcaaacgata
     5701 agactcaaca gcgaggaaga cgtcagacgc tttcacactg actttgaact gatcgaagtt
     5761 caggcgatcg agatgatcaa catgacgcat ggcggattcc agcaacgcct gcggcgtcgg
     5821 ttcgccatac ttttcttgca gatctttttc caacgatccg gcgttaacgc caatacggat
     5881 cggaatgttt ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat
     5941 attgccaggg ttaatacgca gacaatcgac gccgtattcc gctactttca gcgcaatgcg
     6001 atagtcgaag tggatgtcag ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa
     6061 cgcttctgcc gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc
     6121 cagcgctttg atttgattga ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga
     6181 ctgtacggcg atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga
     6241 ttttctacgt tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt
     6301 tactgcgccg gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata
     6361 aaacgactca gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca
     6421 attttcagtt tgtacggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata
     6481 ccgctaaaca gttttttacc ggtagcatcg gtgacttcca gccagcaatc ggaagtaaag
     6541 ttcatcacca gcgcattcgg gtcaaccgcc ggcgtggtca cgccagcctg atcggttggc
     6601 aacggcgcag cgccatctgg tgttgttgtt gccgctggtg ccggagtcgc agcggtatca
     6661 acatttgcct gcgaaggtgg aacaaccgca ttctgttgcg gatcaacagc tggtgctggc
     6721 gcagttacgg caggtgtttg cgtgttggtt gcggtggtat ccacaggtga tgtcggcgtg
     6781 gtcgccatag cctgatctgt ggttgtcgac gtatctaacg gtacactctg gctttgatta
     6841 ttattcagtt ccgcagaaga ttgatcggcc atagtggtga tctcttcctg ctgagctttg
     6901 tggtcttgcc accaccaggc accgctcaga ccaataacca caaacaacac cagccaggtg
     6961 aaggtcatca gccagccgtc acgttttttg cggcgtttac cgagggaaaa actctgcatc
     7021 ggcgcaactt ttgcagcccg tagtggagcc tgcttttcca gccctggcag cagttcttct
     7081 tctggaatat gtaccagacg cgcataagag cggatatatc cgcgcaggaa tgttgaagca
     7141 agatcggcgg gtgccttatc ttcttcaatg tcgcgtaccg tggaaacctt caggcaaagt
     7201 cgctcggcaa cggcctgttg actaagtcct agttgttcgc gagcattacg caggcgagcg
     7261 ccggtagtaa gtgcttcatt ttggtcgtgc gtggcttcag tattcattcg ctgcaggtac
     7321 gtttaaatga gaattgagat gccggtgaac catcatgctc acccacaccg cgaaacatcc
     7381 gttaagttaa ccgttatcat acagtataag accgtctgtg tgctcgtgac aaagcccaca
     7441 ttcgctaagg ctaacttact gttgcatcgt tacatactgc cttaaagtca gcaaaaacgc
     7501 accgttaata ttgaccagac aattgcaact taatacttca ttcataaaag attacgccac
     7561 ggtacataaa gtaaccgtgg cgtaatggct atcagaccgc tttaatgtcg atggcttcac
     7621 cctgcatccg tttacgcagg gtacgtttcg tacggtcgat aacatcgccc gccaactgac
     7681 cacaggcagc atcaatgtca tcaccacgag ttttacgcac aatagtggtg aaaccgtagc
     7741 tcatcagcac ttttgagaaa cggtcgatac ggctgttcga actgcgtcca tacggcgcgc
     7801 ccgggaacgg gttccacggg atcaggttga tcttacacgg cgtatctttc agcagttctg
     7861 ccagttggtg cgcgtgttca gtgccgtcgt taacgtggtc aagcatcacg tattcaatag
     7921 tgactcggcc ctgattggcg ttggatttct ccagataacg gcgcaccgcc gcaaggaacg
     7981 tttcgatatt gtactttttg ttgatcggca caatttcgtc acgaatttcg tcgttcggcg
     8041 cgtgcaggga aattgccagc gcaacgtcga tcatatcgcc gagtttatcc agcgccggaa
     8101 ccacaccgga agtggaaagc gtgacgcgac gttttgacag gccaaaaccg aaatcatcaa
     8161 gcatgatttc catcgccgga acgacgttgt tcaggttgag cagcggctcg cccataccca
     8221 tcatcactac gttagtgatt gggcgctgac cggtgacttt tgctgcgccg acaattttcg
     8281 ccgcacgcca cacctggccg ataatttccg atacccgcag gttgcgatta aagccctgct
     8341 gggcggtgga acagaattta cactccagcg cacaccccac ctgcgaagag acgcagagcg
     8401 tggcacggtc gtcttccggg atatacaccg tttcaacgcg ctgatcgcca acggcgatcg
     8461 cccatttaat ggtgccgtca gatgaacgct gttcttcaac cacttccggt gcacggattt
     8521 ccgccacctc tttcagtttg ccgcgcaaca ctttattgat gtcggtcatc tcatcaaagt
     8581 tgtcgcagca atagtgatac atccacttca tcacctgatc ggcgcggaag ggtttttcac
     8641 ctaaatcttt aaaaaactcc cgcatctgct gacggttgag atccagcagg ttgatttttc
     8701 catctttcgt ggtgacgttt tcaggtgtga ctaattgttc agacatatgc tattccggcc
     8761 tcgttattac acgttatggc ccctggaggg ttgaaaaaag aaacgccccg gtgagcggct
     8821 gctcgtccgg gggcgctgca ttgtacaaat tctggcgcac ggatgccacg tttgcacgcg
     8881 gcatttacga aattattaac gggtgcgcgg gcacacttcg ccttcgccga agaaataagc
     8941 gatttcgcga gcggcagatt cgacggaatc agaaccgtgg gtaccatttt cggtcaggct
     9001 gtcagcgtaa tcagcacgca gagtaccggc cagtgcgttt gccggattgg tcgcgcccag
     9061 cagatcgcgg tgacgctgaa cagcattttc accttccagc acggaaacaa cgatcgggcc
     9121 agaagtcatg aattcaacca gaccatcaaa gaacggtttt ccatcgtgtt cagcataaaa
     9181 gccacgagcc tgttcaacgg tcaggtgcag cattttggtg ccgacaattt tgaaccctgc
     9241 agcttcaaag cgcgcaaaga tactaccaat cacgtttttt gctactgcgt tcggtttgat
     9301 gatggaaaaa gtacgttcaa tagccatttt tacctctgta aattgttctg ttgttgtctg
     9361 taccagcgta cgagatggcg cggattataa tgagcaacag ggccgttgac tattgatgaa
     9421 ggtaacattt ttttaaaata aacccagttt aagcaacaat cagggacaaa acagactatt
     9481 gcatgacaaa tttcactgtc gctatttgcc ccacgtcatc catcaccagc aattgataat
     9541 cgcctttatc cgtcaaatgc agggtcacgt tgcgcccacg ttcagttaac ggttcgccgt
     9601 tcagaaacca ccagcgttca cctgcccctc cacttgattg caacggcaaa gtcgcttccg
     9661 ctgcgcccgg taaacgttta ataatcgcgc catcgcgcac acctgttagc tgcagcggga
     9721 gttgtgcatc gtgaccgtat ggcgggcaaa ttgtcgaggc cggaggcaag cgcacagcgc
     9781 gacgttctga tgcgggcagc cagggttcca gcggtagcgg ccagacattt atcatttctt
     9841 gtcgcgcctg agggcaatca gcggcaacac gtttgccgtt ttcatccagc cagatgggga
     9901 aacgaatgcc attaatgcct tcctgctccg gcagtaatag agttggcggc tggctcccgt
     9961 ccagcagcca ggttgccagg cggcggcgac agttaccgtc accttccggc aaagactgtc
    10021 cgcccggcca gcagataacg ccacgactta cagagtcagg acgtgggtct tccggcaaat
    10081 tcacactgcg cgacagtaag atattattaa cctgattcaa cagcggtacg gcactggcaa
    10141 agccaaactg accaacaacg ggcgtgccgt ccggtctgcc agtccagatc ccaatgacat
    10201 agcgagcgtt aaccccaatc gcccaggcat cacgatagcc atagctggtg cccgttttcc
    10261 atgccagtgg ggcgacgcgc ggcaaggcac catccggcaa gggttgcgct tcatcagcca
    10321 taatccggcg aatgatccac gcagcccccg acgacattaa aggccgttca agcagcggat
    10381 cgtcaggctg taagcgcaat ttgcctgcct tgccgtggcg agcaaacgcg gtatacgctg
    10441 ctgccatatc ttccagtttt gcgccagcgc cgccgagaat gagcgaaaga ttcggcgcag
    10501 caccgttggg cagatacaac ggcaatccaa cattgcgtaa ctttgccgca aaccgtttcg
    10561 gtccataggc ttccagcacc tgcacagcag gtaagttcag cgatcgcacc agcgcctcac
    10621 tcatgctgat cgggccatga aaaccgctgt caaagttgcc aggtcggtaa tcaccggtgc
    10681 gtcgggggac gtcttgcaac agcgatgccg ggtggatcac gccttcatcc agcgccagac
    10741 cataagcaaa cggtttgagc accgatcctg gcgatcggat cgcattgacc atatcaacat
    10801 gaccaaagcg tgaatcatcg ttgagatcaa ccgatcccac ccagccgcga acacgcatat
    10861 cggtatgatc aaccacgatc atcgccagtg agctgcgcgg tggcaatcgt cctttccagt
    10921 tttgcgccag ttcttccaga cgtcgttgaa gaccggcatc cagcgtagta acgattttgt
    10981 cgcttttgct tttaccgagc atcatgcgcg aaaacagcgg tgccagttgc ggcatttgtc
    11041 ggggtgccag ccagatgggt tcttcccttg actcttttac ccgctcacgg gaccacacac
    11101 cttgcacggc catccgttcg agcactttat tacgcgcggc ttcggcacgc tccggccaac
    11161 gatccgggcg aagacggctg ggcgcttgcg gcaacaccgc cagcattgcc gcctcggaat
    11221 agcttaaatt cgcaggcgat ttcccgagat aagcccaact tgccgcacca atcccctgca
    11281 acgtaccgcc aaacggggca cggttaagat acaaggtcag aatttcacgt ttagacagat
    11341 gccattccag ttgcaacgcg cgccagagct gacgaatttt gccgccaaac gttttggggt
    11401 gaggatcaag caggcgcgcc acctgcatgg tgagcgtgct gccgccggaa atgacccgac
    11461 ccgacgtgag atcttgccag gctgcacgcg ccaccgagaa cggattcacc cccggatgct
    11521 tccagaacca gcgatcttca taattgatca gtgcttcaag gtaacgtgga gaaacatctt
    11581 cgattgttac cggataacgc cagatgccgt cagcatcggc gaaacgccag agtggcgtac
    11641 catcctgcgc caccaccacg cgtgcaggat tcacttcttg caatggcaga ggccagagtt
    11701 tatctgctgc ccatgctgcc agaatgatga taaaaggcgc ggctgtcagc attatccagc
    11761 agccgcgttt ggttaacagg cgaggcattt acggtctgac tatcaacaga tcgtcagccg
    11821 caccagtcgc ccgccattga ggaacataca ttgattccac cataggctgc ggcacctgat
    11881 acgttcccgg cgtcaccgcc cgcgccagat acaccagcgt taccggttga tattcatcaa
    11941 cggcaaccgc cgccacaaag cgatcgtcac ggaactctat gtgcttaatg ctcgcctgct
    12001 gcatctggtt cagcaagttc tgcacttcac caccactttg ctccaggctg gcgccgccgt
    12061 tcgccagatt ctggttttcc agttccagcc ccgcaggcaa cagatccacg actaacgcat
    12121 ccggtacact gttactggct ttaacctgca accacaccag caccagatcg ccgctacgta
    12181 acgagtccag cgatttgctc ttaccgtcag tacccagaat atgacgctca atttgcagca
    12241 cattgctcgc cggtaaaggt gcggattgcg gataaccgct ggcatccaca cgcagccata
    12301 acggctgatc gccactgttg gtcacctgca aggtggcaag ttgatcgcta ttcagattgc
    12361 tggtttgcgc tttctcgcct gtcagcggct cagcagagaa agtggtttgc gcctgccatt
    12421 tgcccggtaa atcctgaaga gtacgggcag ccaggaacaa cgcgttactt tcctgcgtcg
    12481 atagccagcg ttcaccgaac gcctgctgcg aaagtgtgtt caacagggag tactgctcat
    12541 cgggtagcag tttgttctct tccagcaatg agagcatcaa cgcgctgtcg cgcagtggac
    12601 taccgtaatc acccagccat atccgctcat cactattacg cggcgttttc agcgccagca
    12661 caatcgcttc ttcaccacgc gttgcatcac ccatgatttt cagcgcaacg ccaagttgca
    12721 gcagcggtaa accagaagcg gcatctgcac gatgctccca gatttcacgc agcgcaccca
    12781 gtggtgcttt ttgctgacgg gccaacacca gcgcagcgta agactgtacg gcgaatttac
    12841 tggctttgag attatcagcg tacgggatcg atatcatgcc cggatcttgt aaatagcgca
    12901 gcagacgctc attaccccgg ttaatggcgt ctgtcggtac gctgtaaccc tgttcgcctg
    12961 cgcggaccag gaaatccatc acgtaagccg tcagccagta ctcttcgtca ccgtttttat
    13021 cccacagcgc aaagccgccg ttatcacgtt gcatttgcag caaacgggaa atgccgatat
    13081 cgaccgatgc acggcgtttc tcatcactgt cgcctttgat gcccaacgcc tgcagttggg
    13141 ctgcattggt ataaagtgac gggaacaggc cgctggcggt ttgctcgaga cagccgtacg
    13201 gatacgcttt taactctttg atataacgtg cgatgttcag cggtggtttg ccgctcaaca
    13261 ataactgccc ttccagcgta acaggcgaga agttttgcaa tccatcagcc gggagcgccc
    13321 aggtttcacc aggctgtaac gccgtaccgt aattgaccgt ttgtgccggg aatgccggac
    13381 ggacgccgat tttccactgc ttatgctgat cggcaacggt ttcccccggt aacgctaacc
    13441 cgctaatggt ggcctgaatg tcaccatcgc cataacccgt cagagcacgc accgggataa
    13501 acagcgtagt acgcacccct ggcgctaatt caacaggtgc gggggaatta ctcaccagtt
    13561 caagcaagcc actggcggtc agagcaacat taagtttttg cggtttatcg gtaagattag
    13621 tgatatccag cgtcaggcgc gaggtatcgc cactcgccat aaagcgcggc atgttaagtt
    13681 cagcaatcac cggtgcggca acaatcactt tgctttcgtt gcttccgaag tcatctgccg
    13741 tccaggcttg cgccatgacg cgcaattcac cgttaaaatc gccaattggc agtgtaaccg
    13801 agccttcgcc ctgttcattg agcgttaccg gcagcgcctg ctgcgcgaca atattgacgt
    13861 gattgaccgg cggtttacca ccacgtttca gctcatcacc atcaccacca aaacgcagag
    13921 ctgccagacg cccctgacct tcaataacct gaccgtaaat atcgtaaatg tctgcgccat
    13981 agcgtttctg accaaagaac gcctgccacg gatctggcgt gacgtaatca gtaatattca
    14041 gcacaccgct atcaacggca gacaccagca cattcacctg ttttggcatc tcaccatttt
    14101 tattgctggc tttaattttc acggttaatg gctgattggg gcgcattttg gtgggggttt
    14161 ccagcgccag atcgaggcga cggttttcat caccaagcgg cagatgcaac accccaaccg
    14221 cgcgttttgg cgtcgcggag cgagatttat cgccaggacg taccaccagc gtacttaaat
    14281 acagatcatg acgattccag gttttatcga ccggaatcgt cagatccagc ccttgagccg
    14341 gaacatcaat ctcttgccac cacaacggcc cttcactgga ctcgaccatc gcataacctt
    14401 tacccgccgt tggcgcggca atatgcaact taatggtatc gccagggcga taactggctt
    14461 tatccagttt cagcgtgaca cggtcgggtc gcactgcacc acccccgtcg ctgttatcct
    14521 gccagctata accagcccag aaacgcacac tactgaccgc ttcattcggc gctttaactt
    14581 ccagacgata agcgccccac tctaccggga aactgacctt accggtttcg tccgctttca
    14641 gatccagagt ttgttcattt tcgatcagat ctttttgatc aaactgtgac tgccagcctt
    14701 catattccga ccagttccag tagtaatcgc ggcgttcgcg aatcaggcgc acctgcaagc
    14761 ccgacacggc ttttttcacg ccttgcgcat cgctataaac gatgtcaaaa gcggcgttac
    14821 tgccttcatc aacaatgggc tgtttcaccg tgctgtcggt gcgataatcg taaaccgatt
    14881 tcgaggcgaa ctgcggacgg atccccggca atgcatcggc aggccagata gcctgctcag
    14941 cacggcgcgt caccgggcga ccgcccgatt ccagcaaact accctggaaa ataacctgta
    15001 atggagaatg tgtttccttc cactggcttt ctgtagaaac ttcgccgcgc cctttatcat
    15061 ccagcgtcaa ctgaacttca tccagcgtgc gggaaagatt ttcggcagct atatcgccga
    15121 attcaaaacc aggtaaggct gacacagctt cacgcagtgg gcgcaggaaa agttgccctt
    15181 gcaaagtatt accattcgca agtgcaccat acaggtagta ccccaccacg gagaatttca
    15241 cttcatcttt cggcgttagc ggggttttct caccggtcag attcagcgcc atacgctctg
    15301 gcataaaatc ttcgacgtgg aaatcccaca tccgatactg gttatcgccc gtgttagcgc
    15361 gaatatgcca cataccggtt gccgcattgc tatcgagtgg ccaggtaaag tggtagaggc
    15421 cattctccgg ctgactaacg acgctcctga gtacctgccc gtcgggttta atcacgtcta
    15481 acttgatggg ttgatcgggc aatgctttac cgtctgcatc acgcagcaaa ccattgagga
    15541 tgaccgtttc acccgggcga taaagatcgc gcgggccaaa catgaaaaac tgtttgctat
    15601 agcctggcgc gccagcaatg ttaaattccg ctaagtccag cgccgggagt tttaaatcga
    15661 gcagcgttgt ctgaccgttt ttacgcgcca gcagtaatgc cgcgttttta tcattttcca
    15721 gttgcacatg cccctgagcg tcactggttg cctgagtcag agtctgccct ttctcattta
    15781 ataaagagac ttcaattcct tgcagggccg cgccgttttc cagactttgg gtaaagatat
    15841 ccagacgatt gtgataacgg tgagctgaaa cgccaatatc acttaacgta aacagcgtcg
    15901 cgggattact gtaatcgtaa cgtccagcct gattcatcac agccagatac acgcccgcct
    15961 gctgaagcgg tttgatatcg cccagcggca gcaataattt ttcacgggtg ttacgcgcag
    16021 gattgagatc aaaccgtccg gtgtagacca gatccgccat ctgcagcagt ttatctgact
    16081 gccagttcgc cagcgaattg cggtattccc actggctaat gaaagcgggc agtgattctg
    16141 gtttcacacg gaagaagtta acatcgacat tgttaacgtt aagcgccatt accggcagcc
    16201 cttcaacgac cttaccagga agcaacgaac cacgactggc aaaaccgacg ctgggttgga
    16261 tatcgcgggt agttatagtt ttttcgtcat ctttactaaa ggttgcgtta ttgagcgctt
    16321 tgacctcctt gccaatagtg acgatcaaat cacgtttcgg ttcgaggtgg cgtaaacgca
    16381 gctctttaag attatctgac agctcccagg caccatccac tttgccgctt tttttatcga
    16441 cgacatgaat aacgcgtgag aaatcctgat ccgggtcgag agggatggag aacgtcagca
    16501 ccagcgtggc tgcgccatcg agttgaactt cagagagatc cagcaatgtc agcgccttac
    16561 cggcactctg ttgggccagt ttttgtcgct ccggcgcgga aagttttgct gaactcacgt
    16621 tttctgaaga ggccgcttta gtaacttcag aaggcgcatc ttttttcacc gctgttggcg
    16681 cgttatcgtt gttgtcgcac cctgccagcg ccagcattag catgcaggcg gctacgcgta
    16741 actttttcat ttttcatccc tgccacaatg gcccgttagc aacgtcgaat aattattatg
    16801 cgtgagaatt tacgttatgt aattcagtat cacgcccata caatccgaat tttagtgaga
    16861 attgtggctc agacagccaa atcagcctct tttgcttgtc gcttttcact aaacatccga
    16921 caatttagcc taaccccggc aaaaatggag atgcctatgt ccacgacatg gtttgtagga
    16981 gccgactggc tcgccgaaca tattgatgac ccggaaattc agattatcga tgcccgcatg
    17041 gcgtcgcctg gacaggaaga tcgtaacgtt gctcaggagt atctgaatgg acatattccc
    17101 ggcgcagtgt tttttgatat cgaagcgctt tctgatcaca cttccccgct tccgcacatg
    17161 ctgccgcgcc cggaaacgtt cgccgtggcg atgcgtgaat taggcgttaa ccaggataag
    17221 cacctgattg tctatgacga aggtaatctt ttctcagccc cacgagcatg gtggatgctg
    17281 cgcacctttg gtgtagagaa agtgtcgatt ctgggtggtg gacttgcagg ctggctgcgt
    17341 gatgatctgc tattagaaga aggcgcagta gagctgccgg aaggagagtt taacgccgcg
    17401 tttaatcctg aagccgtggt gaaagtaacc gatgtattat tggcaagcca tgaaaatacg
    17461 gcgcaaatta ttgatgcccg cccggctaca cgttttaacg cagaagttga tgaacctcgc
    17521 ccaggtttac gtcgcggaca tattcccggt gcattgaacg ttccgtggac ggaactggta
    17581 cgtgaaggcg aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggtgtc
    17641 agctatgaca aaccaattat cgtcagctgc ggttctggtg taacagcagc cgtggttttg
    17701 ttggcacttg cgacgttgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa
    17761 tggggcgcac gggcagattt accggttgag cccgtaaaat aagcttttta cgggcaataa
    17821 aaaaccgccg aatttggcgg ttttttattg ctggtctggt tcgcggcctt tccagcaggt
    17881 tgtattaccg tagtaatgca agcgcgtctc agcggacaca atactcgcca gtaactctct
    17941 ttttgtcaag taaaagagag ttattattgt tctgttagtg tattatccac tgcggccctt
    18001 tccgccgtct cgcaagcggg cgctggctta aggaaaggat gttccgcgac cgttagggca
    18061 agcgcgtctc agcgcttgct atcgcggcaa tatcgccagt ggtgctgtcg tgatgcggtc
    18121 ttcgcatgga ccgcacaatg aagatacggt gcttttgtat cgtacttatt gtttctggtg
    18181 cgctgttaac cgaggtaaat aataaccgaa gtctcttcgg cgacaattta ctggtggtta
    18241 acaaccttca gagcagcaag taagcccgaa tgccgccctt tggggcggca tattttagat
    18301 tatccggttc tgtttaaagt cacgcaaaaa accaccccag cgacgttcat agaatggcgc
    18361 aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct gacaaatatc
    18421 gatgggttca tcaccaggta atgtatcggt cgctacactt cccgccacct gaataatttc
    18481 ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct cttcgttctc
    18541 tttaattgaa caaataaaag cacgcttcac cggcttaatg gttttaaata aggtggtaag
    18601 tgaatcaatc atttgcgctg gcggctctgc gacttccgat aatatcagcg attcaccgcc
    18661 ttccagtact tcctggctac tcagcggatt cccttcttca ccaatcaaca aactgatttc
    18721 acgcggcata aactctttac cggttggcag tttggcatta aggaagagcg tttcgccaag
    18781 ggtcatctca aacaatgtgc gaacgggcat tacgacaaat gcctgttcgt cttcaaccgc
    18841 ctgttgaagt gcttctaacg aggtaaaaaa aggaatgacg ctggtgccgt cttctttttc
    18901 ccagtgctgt aaatcaagcg cgctatcttc aactactgcc tcgccctgcg ccgccgtacc
    18961 aggcacccag acggtggatt ccagtagagt acggaaaaag gccgggcggt gcgccggttc
    19021 ggttgctgct ttttccagca ggtcttcaag ttcgtttttt gtttcggaca taagaaccac
    19081 aattcattca acgttcgggg agcaagtgcc tgatgcgcca cgcttatcag gcctacaagg
    19141 gagtcgcaat ctattggatt ttcgcaattt gtaggccgga taaggcgttt acgccgcatc
    19201 cggcatatta gtttacgccg ttaacagatt agcaatcgtg cgcacaccaa gtcccgtagc
    19261 acccgcagac cactgttcaa ccggcgcttt acggtaagtc gccgagcagt cgatatgcag
    19321 ccagccttgc tgatagttct caacaaagtg cgacaggaag cccgcagccg tacttgcacc
    19381 tgccggatac gccgcgcttc cggtattgtt cagttcggca aagttagacg gcagctggct
    19441 gcggtggaat tccgccagcg gcagacgcca gaacggttcg ttctcttgtg aggcactcgc
    19501 cagcaaacga ccggcaagcg catcgtcaaa actgaacaac gcgtgataat cattacccag
    19561 cgcagttttc gccgccccgg tgagggtcgc cgcatcaatg atcagttccg gtttctgcgc
    19621 actggcatca atcaggccat cggccagcac cagacgccct tcggcatcag tgttcatcac
    19681 ttcaactttt ttaccgttgc gataggtgat gatatcgccc agcttgaacg cattgccgct
    19741 gatcaggtta tccgcacaac agaggaacag cttcacgcgc ttgttcagtc cgcgcgtaat
    19801 ggcaaatgcc agcgccccgg taaccgttgc cgcaccgccc atatccgact tcatcgagtc
    19861 cataaacgcg gtctgtttga tgctgtagcc gccggaatca aaagtgatgc ctttaccaac
    19921 caggcacgcg tacactggcg cttctttatc gccagttggg ttgtaatcca gcgccagcaa
    19981 taccggagaa cgttctgaac cgcgcccgac agtgtgcagc cccatataac cttgctcacg
    20041 cagatcttcg cctttggtga tccggtaagt cacacgatcc cctgcgacgt tgcagatcag
    20101 atcaacagca cgctgtgcca gttgcgatgg tcccagctct tctgccggtg cgttgatggt
    20161 gtcacgcacc cagtcaatga tcatcaggcg gttatccagt tcctgacgct gggcatcgtc
    20221 cagctctggc cactccactt tacgtgtgcc tttcggggct ttgtaacctt gccagaatgc
    20281 ccagcagcga tccgcatccc agccttcacc gcttaactga acatgcttaa tgcccagacc
    20341 atcaaccttg cgcgccgcac gctggatcaa ccccagatcg tctgccccgt tcaaatgcag
    20401 ggtaatgcca tcattattaa tgctgtaagt tgctttttct ccccagcgca cgtcggcagg
    20461 ttgggtagaa agggtaattt tcatcgcttc tgtcatttta gttatcctta ttcgtttaac
    20521 agcaaaacgg gctaccccca ggcagcccgt tcattcactc ggcttcgtcc agccagacca
    20581 acaaaatcgc ttcgaggatt ttctcgttcg atgcctgcgg gtcgtcgtcg aaatcttcca
    20641 gagcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca atatcgggat
    20701 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt cccatactaa
    20761 cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc
    20821 ttcgtcggta acgcgcgcct ggcagcttaa acggctttcc ggctccagcc cccaggcttt
    20881 gtccagcatg tcgtcttcct gctctgagct ttccggcagc gagtcaaaac cttcgcgaac
    20941 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcgatacc
    21001 gttacgcagc gcaacatcga gaatggtttc accgctattc gcttccagaa cagcgccatc
    21061 agggcagaga tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
    21121 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
    21181 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg ccctgcgcca
    21241 cttcactcag gtgagcggca gcatcgtcga taacctgacg ttctgcggcg cttaacagcg
    21301 cggcatcagc agccagcgca ccgtgcagac tttccagcac acgcgccgct tctacttttt
    21361 gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag tctttgatca
    21421 tcgaggcgat ttcgctatcg gtcaggccat aagacggttt gacctgaata gacgcctcaa
    21481 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
    21541 tcacgcgaat atgcgcaccg ccagccggta gcgccggaat acctcgcagc gcaaaacgcg
    21601 ccagcgagcg gcagtcctgc accagttcgc gctcaccctg catcacatgg atagacatcg
    21661 ccgtctgacc atctttaaag gtggtgaaat cctgggcgcg ggccaccgga atcgtggtat
    21721 tacgcgggat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagcggga
    21781 tcacatccag caacagcatt tcgctgtccg gcttgttacc caccagaata tccgcctgaa
    21841 tcgccgcgcc aatagcgacg actttatccg ggtcgataga agtcagcggt ggacgaccga
    21901 aaaattcgcc tactcgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
    21961 cttccagcac ttcatcagct tctacgcccg catctttcag cgcgcgacga caagccagta
    22021 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
    22081 gccagcccgc aacgttaacg gtcacagagt ccgcatcgct cagcgcgatt ttggccgcaa
    22141 tggcagcatc gagcagttca cgctgaacgc ggttatcgct acgatcagga atgcccgcct
    22201 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac
    22261 cgccggtcgc cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg
    22321 tcccgccgcc gaggtcataa acagcgatca cgccttcctg gccggaatcc agcccgtagg
    22381 caatagccgc agcggtcggt tcattaagta agcgcagaac atgcagcccc gccagacgcg
    22441 ccgcgtcttt ggtgccctga cgctgggcat catcaaagta cgccggaacg gtgataacga
    22501 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
    22561 ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccat
    22621 tctcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
    22681 agcgccccat caggcgttta acagaactaa tggtgttggc ggtgtcgagc gctgcgttag
    22741 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gacggcagca
    22801 ggtgacggcc ttcatggtca gctaacgttt cggcctgacc gctgcgcact gttgccacca
    22861 gcgagttggt tgtgcccagg tcaataccgg ccgccaggcg acgctgatgc ggcgcagcac
    22921 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
    22981 agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa acgcagctta
    23041 cgcacagtat ccgccgccgc gtcccacgtc tcgttgtcta actgttcaac catcaactga
    23101 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
    23161 ttcgcctgtt cgatctcgtc cagttcttcg cgcagttcca gctgttccat caggaacgcg
    23221 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgcaaaga aagcaaatat
    23281 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt cgcggattgc
    23341 tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg atgatactga
    23401 cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata acgggcaggc
    23461 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
    23521 gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
    23581 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt gacgttcggg ttggtgaatt
    23641 taaacccttc gttaaggcct tctttgacga agtccagctg cgtaccgtcc agaaattgca
    23701 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
    23761 tcggttcgtc aacaaattcc agtacataag ccatacctga acacccggag gttctcacgc
    23821 ccagacgcag gccaaaccct ttaccgcggt tagccagaaa ggtatttact cgcgctgctg
    23881 cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
    23941 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
    24001 ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg
    24061 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcggaaccg
    24121 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
    24181 aacttcatca cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg
    24241 ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
    24301 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
    24361 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
    24421 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
    24481 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
    24541 taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaaccgc gaggtctttc
    24601 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
    24661 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
    24721 agacgattac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
    24781 cgataggctt cgcccatacc gacgatctgg tgaacaggca gagtgccgga acgcataccg
    24841 cgctcgtgac cgccgccatg catttgtgct tcgatacgta cgcgcggttt acgacgcaca
    24901 tacagcgcac caataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
    24961 actttcaact ggctcagatc gatcggcagt ttgcccacgc tctgggttgc atcaacgtga
    25021 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
    25081 atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgctgcttcg
    25141 agttctttca ggtcgataat accgttacgc tgcggtgcca ggtaagtgac ttcaaaacct
    25201 tcgcgctcca gctgacggca ggtatccagt accgctttat gttcggtttt actggtgatg
    25261 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat
    25321 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
    25381 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgggag
    25441 gccgggttac caaaggttcc gtccatcgtc ataaactgca tcattttctc ggcaacacgc
    25501 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
    25561 actccgtaca tcactcaatg caaggaacca ggctaccggc tggatgtacg accgtgttta
    25621 cggagtgttt agcactccgg cctgattctg aattcttttt attaagcgcg taacttaacg
    25681 tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca
    25741 tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttattgag aaaaccggta
    25801 agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag
    25861 ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacctcg
    25921 ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg
    25981 ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa
    26041 attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca
    26101 acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc
    26161 acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag
    26221 ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg
    26281 tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc ggatgcggtg
    26341 tgaacacctt atccgacctg ggattccgtg cacttacagg cctgataaga cgcgttaagc
    26401 atcgcatcag gcaaccactg ttattcggcc ttgttacctt tattctgctg ctcaatagaa
    26461 gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg ggtaaacaga
    26521 cggcgcaatt tattcatcac ctgtcccgga tggttttcac ggataaaacc ggttgccagc
    26581 agcgtttgct ccagatgacc ataaaaacgc tccagatcat cgaccagcgg atacggcgtc
    26641 tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat gcgcacttca
    26701 taggcaataa cctgaaccgc catcgccagg ttcagcgaac tgtattccgg gttagccgca
    26761 attgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac gcgctcgcga
    26821 ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag accgcattcg
    26881 cgcgggtcga gcatcggcca cggcagcgtg cgggagcgag cgctggtgcc caccaccaga
    26941 ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc aatcacatcg
    27001 ctggcccctg ctgccagggc aatcgcctgg gagtcaggtt tcactagtgg attaaccagc
    27061 cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat attgccggtg
    27121 tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct tcatctaaag
    27181 attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc tatactctgc
    27241 gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat gctgaacatc
    27301 gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga aaccccggac
    27361 gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga taaagctgcc
    27421 gaagcggtga ttatcgacac gattcgtaaa tcttacccac agcacaccat catcaccgaa
    27481 gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga tccactggat
    27541 ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc cgttcgtatc
    27601 aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact gttcaccgcc
    27661 actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggcagcac cgctcgcgat
    27721 ctcgacagta ctattctggc gaccggcttc ccgttcaaag caaaacagta cgccactacc
    27781 tacatcaaca tcgtcggcaa actgtttaac gaatgtgcag acttccgtcg taccggttct
    27841 gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt tgaaatcggt
    27901 ctgcgcccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg cggcatcgtc
    27961 agcgacttca ccggtggtca taactatatg ctgaccggta acatcgttgc tggtaacccg
    28021 cgcgttgtta aagccatgct ggcgaacatg cgtgacgagt taagcgacgc tctgaagcgt
    28081 taatgactca ggcgggtgat atcactcacc cgccctcgcc tttcaggcgc tattccgaaa
    28141 tacttcctca ccgctttact ttctttcgtc actctcccac catctttcgc cgattaatgg
    28201 atgataaaga agtcgcaagg aaaggttatg gcgctgccag tgaacaaacg cgttctcaaa
    28261 attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg cgtcgccatc
    28321 aacttcttct attatcctga cgataaaata tacggtcccg acccctggtc ggcggaatcc
    28381 gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc ttcttcgacg
    28441 ggccctgctg acaacgccat cgcaaccatc attcatgctc acggcaatgc cggaaatatg
    28501 tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt ttttatgttt
    28561 gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc tggccggatt gctggacgat
    28621 acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca acggctggtg
    28681 ctgttcgggc agagtattgg cggggcgaat attctggatg ttattggtca gggtgatcgt
    28741 gaaggcatac gtgcggtgat cctcgactcc acctttgcct cttatgcgac cattgccaac
    28801 cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga aaattacatc
    28861 gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca cgttatccca
    28921 tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc caaaacggtt gatcctaatc
    28981 ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta tcgcgaacag
    29041 atggtggatt ttatccttag cgcattgaat ccgcagaact aacccatgat cgctagcacg
    29101 ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac agtttaagat
    29161 gagaaaaatt ctgtgatgct tgccaacatt tctgatgatt agcattccct tcgccatttc
    29221 cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac agatgatgcc
    29281 tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga tcttgctgac
    29341 gctctttcag ccggggttga ccgccaccac ggcaactttc agcgagctta atggtgtgga
    29401 tgatgatatc gccagtcttg atatcagcga aacaggacgg gagatcctgc gctatcatca
    29461 actcacactg acgactggtt atgacggcag ctaccgggtt gaaggtacag tgcttaacca
    29521 acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt catttattac
    29581 cagccgattc acccccgccc tgaagagtga actgaagcgg cgcggaattg cgcgtaactt
    29641 ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc tgcaaaaacg
    29701 ctttgaaacg cgcgatattc atttcctgtg tctgtatttg caatattgtt tgttgcaaca
    29761 tcacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg aatcctgcct
    29821 tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc tccagcctgt
    29881 accgcctgat gagccactat ttatggcact acttttttcc atgttgcggg ttcccgatcc
    29941 actgcgggat gcgcatcagc gggacagaca actgcgtcag tctatcaaac gtctggtaaa
    30001 ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat gcgatcagct
    30061 ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta ttgataacac
    30121 cctgccggaa gagttcgccc gcctgtatcc acgcctggtg cgcaccaccc gcgcggcgct
    30181 ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg gtctggtcgc
    30241 ggtgattttt ggtgcctggc taatgcagga aaacgacctg catgagaaac agattattct
    30301 actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagctac gcgaactaac
    30361 gttactgccg ctcaacatta aacatatgtc ggtaaaggca tttttgcaga caggcgctcc
    30421 gcgcggcgcg gcactgatta ttgcgcctta taccatgccg ttaccgctct tttcaccacc
    30481 gctgatctat acggacctga cgttgacaac acatcaacag gagcagatcc gcaaaatgct
    30541 cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc gccaccagcg
    30601 ccatcaccca gaacacgccg tggccgagat attgatacag gaaaccggcg aaaacggtca
    30661 tgatggcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa cggatgactt
    30721 cgctaccctg gcgagcagca atatagcgca tggcagccag gtggcagacc gtgaaggtac
    30781 cgcaatgcag aatttgcacc actatcaacc acggcaacgc cgtcgttgct cccataatgc
    30841 cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgtgca ctacaacggc
    30901 ggaaaagttt attactcagc gcaaaaataa tgacttccgc caccacgccc agcgaccaca
    30961 aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg ctaaaaccgt
    31021 aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc cagttctggc
    31081 gaaccaacgc caaccacgca gaccagccgg tgctctcctg ctggcggctt gccccttgtg
    31141 gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc aacgtcaaca
    31201 gcgcgaggat cacccgataa tcaaacatac tgaccagttt gcccgtcagc gccgagccaa
    31261 tgacaaacgc caccgagccc cacagtctca ctttgccgta atcaagcggg aactgctttt
    31321 gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag aggttaaagc
    31381 caatcatcac cagcattagc cacgctacgt gcgcccccgc ccagaaggcg acagcaaaga
    31441 gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga tcgctgacgc
    31501 ggggcgcgat gagcaaactc ccgaggaaac gggcaaccag acccgccccc aataacaggc
    31561 cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa ggtagaaaaa
    31621 tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg gattgcaaaa
    31681 ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt ggtgcaagtt
    31741 gaaaaataag aatattcgag cctgcttcct gacggttatc ctgcatatca ggaaatgaca
    31801 aaaaagtgcg gcccacaaca caacatgtaa aaaagaacga tgaaacaggt gaaaatcgca
    31861 atttattgaa ctgacaaact catgtgagac ggataaggcg tgcatgtcgc attcggcagg
    31921 aaacaataag cacattgtca gccatctgcg ttatgccgtt ataccagaaa cgaatatctc
    31981 aacgcctggg gcgtggcgag gtaccagagt gtgtattacg tttaaatcac atcatcttgc
    32041 aaagggattg gttatgaact cactacgtta ttttgatttt ggcactgccc gccccgtttt
    32101 attattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    32161 gatgggcttt gacggtacgg tccaatatat ggcctcgctg ggcgcgccaa tgccgatgct
    32221 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    32281 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    32341 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    32401 gaatgtcagt atcgctggcg cattcctgct gttggcaatt accgggccgg gggcgatttc
    32461 tctcgatcgc cgttaggaaa gatgccggat gtggcgtgaa cgccttatcc ggcattttat
    32521 aaattacagc aacgcaaccc gcaacgacat ctctgaaatt gttgattccc ctggtgcaag
    32581 cgcaatgagg tcaccacctt ccgggcgatg atggtcatcc ggcgcatgac tcatcggttc
    32641 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtctgaaa caaagatgaa
    32701 ataacacggt gcaggtggcg tcgtttccat gatgatggca tacccctcct gcggctgctc
    32761 gatgcgggcc ttaccattcc atccggcaaa accattgttt acccactggc gcggcaacgg
    32821 cgcgagctgg ttaaaatcca gctcctgcgg tagctgctcg caaaactcac ccgccagcca
    32881 ctgctcccgc tccagccagt aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa
    32941 cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac
    33001 agcgagcgtc accgtcagcg tatccgccgt taaatgaaat gcctggctta cccgatagtg
    33061 ataaacaccg ctgagatgtt catacaccag acacaagcta tcatcgctgc gcgagacaca
    33121 ttgccactcg cccaaccagc catcgccgtg tagatagtgc gcatcccact caacattcgg
    33181 ttgcagttga tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa
    33241 tggtaccagc ggaaaacaag aggcatcggt tgccacaccg ctttttttac caggacgtag
    33301 cagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctgatcag aaacgtccag
    33361 ctttagcggc ccgtgagata aggtatagat ggtcatccgc agactcctta atccgggaaa
    33421 ttaatcacaa ctttcccaca tttgccgctt gccatcagtg cataagcatc tcctgcctgt
    33481 tccagcgaga aacgatgggt aatggcgtta cgcggccaca gtttccagtc cgttaaatcg
    33541 tgggcgcatt tttccatatg gaacaaactg gtcacccagg agccaataat ccgccgttga
    33601 tggtgcatca ggtcagcgct aacctcgaac tcgacttttc cggtttcacc aatgtaaacc
    33661 acgcgtcccc agtcagctgt ggattgcagt gccagtaagc gacctgcggc attaccggaa
    33721 caatcaagcg caacatccgc gccgccgtga gtgagttcgg cgattatctg cggcagacct
    33781 tcggtggtcg ctaaatagcc gtgatccatc actcctaact gtttcgccat cgccagacgt
    33841 tccggcagca tatcaacgcc gatgatcctt tttgcaccac gacctttcgc cagcatcatc
    33901 gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctacc ggaaacttcg
    33961 ccgcgcaaaa tcccttcata cgctgtacca acgccgcaac tgataaacgc accatcttca
    34021 tagctcagcg cgtccggcag caggatcaga tctttttctt ccgccagcag gtattcagca
    34081 tggccgccgt cacgctgcca gccgtaagcc gcttttcctt cgccagtaca agaaatagga
    34141 aaaccgcgac ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    34201 tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca
    34261 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt agcgcggtgc
    34321 tggtggtaga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    34381 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt cgaatttcct
    34441 ggtaaataag ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc
    34501 atccctgccg cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctactacgct
    34561 gggtcagcaa gatatttgcc agcaccgcca ccacaatgat gacgccgcgc accacctgct
    34621 ggaaaaagga gttaatcccg agcagcacca gaccgttacc gattagcgta atcaccagta
    34681 cgccaagcag cgtaccgaac aacgagccgc gaccgccgga aagcgccgta ccgccgacca
    34741 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga
    34801 ggcgcgccgc caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaaaataa
    34861 gaatgcgtac ccgacgaacg ttaataccgc acaactgcgc tgcagtggca ttaccgccaa
    34921 cagaaaaaac cgagcgcccg aaggcggttt tgcggctaat gaacacaaac agcgcaaata
    34981 acaccatcat gatcagcgcg gataccggca caccgagaaa ttgtccgccc agccagtcca
    35041 gcacttcgtt ttcgttaatt ggcactggca aggcgttcgt cataaacagc cccattccgc
    35101 gcagggcgct ccataaaccc agcgtggcaa caaaacttgg cacgttaaac acgccgcgca
    35161 gcaccccggc gagcgttccc atcagcgcac ctaacagcaa caccagcaga caagcaatcg
    35221 ccagcggaac ttcaaattgc agcaaaaacg ccaggcacac tgaaacaaaa gccaccatcg
    35281 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcga
    35341 tcccgatggt ggcggcatcg cgcagcacgt tcatttggtt attcaatgag ataaagccag
    35401 gcgcattcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    35461 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    35521 acgaagcaga catcttcagg ttcctcggta aaattcatca gtggacagaa tggcggacat
    35581 cagctcatcc acattgaccg gcgagtgaaa ctcctgcgag aacgtgccgt gctgtaataa
    35641 caggatgcgg tcacacacca gcggcaactc ctccacttca ctggagataa acaccacgct
    35701 ttttccttcg gcagccagct cgcggacaat acggtaaatc tgctgtttgg cttcgatatc
    35761 gacgccgcgc gttggctcgt cgagcaacaa aatctggctg gcggcataga cccaacgacc
    35821 gatcaccact ttttgctgat tgccgccaga aagcgtgccg atgggcgttt cgctactggc
    35881 ggccttgacc gtcatccgct gcatcacctc ttcggtcagg cggcggatgg tggaccattg
    35941 cagcacaccg ttggcgctga ttttttgccg attggtcagc actgtatttt cgtcaacgcc
    36001 caaccaggga atgatccccg cttctttgcg gttttctggc gtatagccaa tgccgcgttt
    36061 cagcatgtcg ccgtaatcgg ggcgcatgat tttctcgccg ttgataacaa tttcgccctg
    36121 ttcatacgtc tccagaccaa caatcgcctt cagcaattca ctgcgccctg cccccagcag
    36181 accagcaatg ccgagcactt cgccacgacg tagcgtaaaa ctgatatcct ccagcttggg
    36241 cttatggcgt aacgcacgga cttccagcac ggcttgatcc ataatttcct gaggggcaac
    36301 cggcgcaata tcaacgtgat cgcgcccgag cattagcgac acaatatgat gcgtagaggt
    36361 gttttcgagc atcacatcgc ccgccacctg accatcgcgc ataacggtgg cacaggaggc
    36421 aatgcggcga atttcttcca tccggtggct gacataaatc accgccacgc ccagtgccga
    36481 catctttttc accgcgctga tcaccagttc aacttccgca ctggcaagcg agctggtggg
    36541 ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc cgcgcaattt ccaccagttg
    36601 cttttgcgcc gggcttagcg ttgaaacaag ttgttcaggg ctaacgtcaa cgcccagcgc
    36661 ctgtaagcaa cgttgggcat cctgcgccat ttgcaggtaa tcaatcatgc cgttgcggcg
    36721 gggccactga ccgaggcaga ggttttccgc cactgtcagc ccttccacca gacttaactc
    36781 ctgataaacc gcgcgtaccc ccagttcagc ggcacggcga gtcagcgtag cttcgtcacc
    36841 ttccagtcgc gtctcgccaa tccagatatc accgctatcc ggacgttcgc taccggtaag
    36901 cattcgaatg agggtcgatt ttcccgcacc gtttttgcct aacagcgcac gaacttcgcc
    36961 ttttttgagc gtgaagttaa cgttatccag cgcaacgact ccgggataac gcttatttcc
    37021 tgccaccact tttgctaccg ggactgcctc tgttgccgtg aacataggga cctctgcgaa
    37081 tcagcgatta gggcagacca tcaacgtgcg ttgccagcca ctgtttaccg tcttccgttt
    37141 tggtatagag atcgattggc acctgaatca ctttttcacc gtcggcttgt ttattgataa
    37201 ccttcaatgt ttgcgtaaaa acagcattgc ccattttctt accagaaata tccaccaccg
    37261 ctttcagcac ctggttgttt tccagctcct gagcaatttc agtggtcata tcagaaccga
    37321 aaacagcaat ttttccggcc tgattttgat tacgtaccgc ttttaccgcg ccgagtgtcg
    37381 ctccgcccga ttcccccata atggcgttga gatccggcgt ggagataatc agtttttcac
    37441 cgacagaaat cgctttatct aaaacagtcc cttcctgatt agcgacaatt tgcgcgccgg
    37501 gaacgcgggt ttttaatact tcttcaaacc ctttacgacg ttgcacacaa acttcaaagg
    37561 cttcgcaatt gatgacggca attttcggct ggtcaatttt gttggcaata aaataatcgg
    37621 cggcagcgtt acccagtttt ttaccaaatt ccagcgggtc accaaccaga tatgccgaga
    37681 catatttatc gacacccttt tgattaatac aggtgttgta gcaaatcacc ggaatacccg
    37741 cttcactggc gcgacgaacg gtacggctac tgccattttc agacactgcc gataaaataa
    37801 tggcatcgac attacgcgcc acgagggtat caacaaaggt actttctttc gaaatatcgc
    37861 cctgagcgtt agtttcaatt aactgtacct gtactgaaga atcttttgcc gcatcctgaa
    37921 cgccctgacg cactccggcg taatatccct gggtatcaag gtatattgcg ccaatagtca
    37981 tttctttatc agaagccctg gcaaatagtg cgctacccaa tagtgtagcc attaataata
    38041 aattacgggt tcttgtcatt tttttaggca tagtgcctcc tgtagggttt ttatttacaa
    38101 cggcttattt taattatttg agcgtgagcg gcagcacgtg ccgctcaaat attacagtta
    38161 atagggttta gtgaacgtag ctaaaaataa aaggcatcac ggtggtgaat aatgccgctt
    38221 tcggccaggc tggattaatc gccatcaatt cagcgcgagc agattcatag tgcgcaacct
    38281 cccctaatcc cgcggccgcc agcatcgcca ccatcaggca tttttcttta tgctgctgtt
    38341 gtaaatcgcc atacagcgac aacaagtcag gctgcgagac ggcaaagaaa tccgcctcga
    38401 tactggtttt cgccatctct tgtgcccact gtttcatttc actaaacagt tgctgtgcag
    38461 tttgttgttc gcccagcagt cgcagcgcca ttccttgcca gaagagataa tcaaccggct
    38521 gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc agacgtaaac
    38581 aacgcgtcgc ttcagtttca tcgccctgcg cgttggcgca tactgcctgc cagaaccaga
    38641 tgtcgttgtc agtttgcccc ggtaaacggc cttcgcttaa attctccgga taatgcagcg
    38701 cggcatgaag cagttcgctg gcctgctgcg gctctctgtc atcaagatgc tgccaggcgc
    38761 gtaataactg gttgaggata aactggctgg tgaccttccc ttccccacct tcccacgggt
    38821 ggaatttgcg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg agatgccaca
    38881 aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttccaga ttatcttcca
    38941 gacgcgccag tcgtttctcc ggtgtgacac cgcttaactt atccagcaaa tcccgttcga
    39001 aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa cgcgcggcca
    39061 gctcataatc gtgctgctta ttccacgcat ggatcgccaa cccgcgccag ccgtcggcaa
    39121 actccggcga catctctacg caacgctgcc ataaggcaat ggctttgccg tagctacgct
    39181 tgttgtagta gaagcaggcc agtaaatgcc gggcaaacca gcactcttcg atactctcca
    39241 gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag gcatcaatag
    39301 cttttgcgac cagttctccg cgttcagctt ttggtagcaa actggcttgc aggtaaagcg
    39361 gtagcgtgcg ctgacagtcc agtgcgttca gcatatctgc cgccagggcg ggcatgcccc
    39421 agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc gcgtccctga caaagacctc
    39481 gccactgcgc gagggctgat tcgctacgac catcgaacca gttcagccac cacagagtgg
    39541 cgttcagcgg ataatcacgc agcagtttct cgcgctgcaa acgcgcgttg tcctgacgac
    39601 cacttaacac cagtagcagg ttatgcaggc aaagcacttc ctgattggtt gggcaggcgc
    39661 gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc gccgccagtc
    39721 gtgccagacc ataatagccg cccgctttac tgttgccgct ccagaccgca cgccagaaat
    39781 cctcttcggc ttgttgatat tgcccctgac gttcgtaagc actggcgcga atcaaactcg
    39841 cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagtgcc tgactggcat
    39901 ataccaccgc ttgcgggaaa tctgcacggt tatattccag cattgccagc gccagattac
    39961 agcgataatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac ggtgaacggc
    40021 tggcgtgatg atattgctcc agatgttgac cgataaacca ggcttcatct gtactggtaa
    40081 tgtcttgtgc tgccagtggc gctttggcga cgtccggcaa cggcaacgct tgcggctgat
    40141 gttcatgata actcagtaca atattgccgt cggcatcgga gagctcaatg gtcagccttt
    40201 caggattgat accgtgcaac acgccctgga tggcggtcgc tggtgtcagg gccacggcat
    40261 catcgagcaa cgcgttgcat ttgccgattt cgcggatcgc caggcggtat ccgttcaacg
    40321 gagagatggc atacagcccc cactcaatcc cccgctcact acgctggagt tttatcaccg
    40381 catcgcggga ggcattttgc accatgccca gagaatgata aggcaggaaa tactgctcga
    40441 aacgcttctc ctcgtaagca tcaagccagg taaaatcagg ctggttatcg gcaaaaatac
    40501 cggtcatcag ttcgatatac gggccgttat tgtcagtcag gctcttatcc cacgcctggc
    40561 caaattcact gtgtccccag ctccactgtt ttttacctgg cgcaatatgg tggttggcaa
    40621 cgtgtagcaa accaccatct tcatcgtgac accacgcgcc aacaaaatcg tactgtgatt
    40681 tttcagccat atatgaggtt ggaacgggca catttttata gcgagaaatg tccactccgg
    40741 cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag acggcccgtt
    40801 tgccgtgatc aaacaccgcc gttacatccg gcgggaagac gctctgatgc ccttcccccc
    40861 ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgttg ccgttataga
    40921 cgcgactggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct gtcatcacct
    40981 gtaaaccgtg catcgactcc gtttcgccga cccacaccgt ctgtgcgccg tcgtcatggg
    41041 cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atggtgttgc ggccagttaa
    41101 actcaatccc gccggagatc cacggcccca gcagtcccac cagcgccggt ttaatgactt
    41161 cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc acccgaccgc
    41221 ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc gcctgccagg
    41281 atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa accgcgccgg
    41341 acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc ggcccggttt
    41401 cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg gtgccctcaa
    41461 tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa tctggcaatg
    41521 ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga gagcctgcat
    41581 taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc tgtaccggca
    41641 aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc cggcggtcag
    41701 taatattttg caggaactgg aaagcgaaaa acgggtggtg aatatcgacg atgaaagtca
    41761 aacgcgcgga catagtagcg gtacatggct gattgcgccg gaaggtgact ggacgctgtg
    41821 tctgaacgtg acgcccacca gcattgagtg tcaggtcgct aatgcctgtt taagtccgaa
    41881 aggcgaattt gagtatttac agattgatgc accgacgccg caggcgctgc tgtccgaaat
    41941 cgaaaaatgc tggcatcgcc accgtaaatt gtggccggat cgcaccatca atctggctct
    42001 ggcaatccac ggtcaggtgg atccggtgac cggcgtgtcg caaaccatgc cgcaagcgcc
    42061 gtgggcaaca ccgattgagg tgaagtacct gctggaagag aagctgggta ttcgggtaat
    42121 ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata attcgcaagt
    42181 gcgtgatttc tgcgtaatca acgttgatta cggcattggc tcgtcgttcg tgattaacga
    42241 gcaaatttat cgcggcagtt tgtatggcag tggacagatt ggccacacca tcgttaatcc
    42301 ggatggcgtc gtctgcgact gcgggcgtta tggctgcctg gaaaccgtcg cctcgttaag
    42361 cgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata ctcagcttaa
    42421 tcctgaaaaa ttgactaccg cgcagttaat cgctgcctgg cagagtggag aaccgtggtt
    42481 caccagttgg gttgaccaca gtgccaacgc cattggtttg agtctgtata acttcctcaa
    42541 catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg gtgagaactg
    42601 gcttaatacc attattcgcc agacgggatt taacccgttc gaccgcgacg aaggaccgag
    42661 cgtgaaagcg acacagattg gctttgggca attaagccgc gcacaacagg tactgggaat
    42721 tggctatttg tatgttgagg cgcagttacg gcagatttga tggcgcgata acgtagaaag
    42781 gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg taaaccgggt
    42841 aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga
    42901 tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc
    42961 cacgacgggt aatcgccgga gtacctacac gaataccgga agtcacaaat gggctcttcg
    43021 gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg
    43081 cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtac
    43141 cgccggaaac cactttgtag ccgcgttcga ggaacacttc taccatcgct ttggcgtttt
    43201 tcgcgacctg ctgctggtaa gttttgaact caggttccat cgcttctttc agagcaaccg
    43261 ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt
    43321 tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg ccgcgcggac
    43381 ccgccagggt tttgtgagtg gtggtagtta ctacgtgagc atgaggaacc gggttcgggt
    43441 agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg taagcaccga
    43501 tgctgtcagc gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaagc
    43561 caccgataat cattttcggt ttgtgttctt tagcttgttt ttccagatcg gcgtagtcga
    43621 tatgaccagt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa
    43681 ccggagaacc gtgagtcagg tgaccgccat gcgccagatt catacccaga acggtatcgc
    43741 ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga
    43801 cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga
    43861 tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt
    43921 tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga
    43981 tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg
    44041 catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac
    44101 aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa
    44161 tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac
    44221 gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga
    44281 tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc
    44341 agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaaaggaag
    44401 accatatgct tgacgctcaa accatcgcta cagtaaaagc caccatccct ttactggtgg
    44461 aaacgggacc aaagttaacc gcccatttct acgaccgtat gtttactcat aacccagaac
    44521 tcaaagaaat ttttaacatg agtaaccagc gtaatggcga tcaacgtgaa gccctgttta
    44581 acgctgttgc cgcctacgcc agcaatattg aaaacctgcc tgcgctgctg ccagcggtag
    44641 aaaaaatcgc gcagaagcac accagtttcc agatcaaacc ggaacagtac aacatcgtcg
    44701 gtgaacacct gttggcaacg ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg
    44761 cgtggggtaa agcctatggc gtactggcta atgtatttat caatcgcgag gcggaaatct
    44821 ataacgaaaa cgccagcaaa gccggtggct gggaaggtac tcgcgatttc cgcattgtgg
    44881 ctaaaacacc acgcagcgca cttatcacca gcttcgaact ggagccggtc gacggtggcg
    44941 cagtggcaga ataccgtccg gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc
    45001 cgcatcagga aattcgtcag tactctttga ctcgcaaacc ggatggcaaa ggctatcgta
    45061 ttgcggtgaa acgcgaagag ggtgggcagg tatccaactg gctgcacaat cacgccaatg
    45121 ttggcgatgt cgtgaaactg gtcgctccgg caggtgattt ctttatggct gtcgcagatg
    45181 acacaccagt gacgttaatc tctgccggtg tcggtcaaac gccaatgctg gcaatgctcg
    45241 acacgctggc aaaagcaggc cacacagcac aagtgaactg gttccatgcg gcagaaaatg
    45301 gcgatgttca cgcctttgcc gatgaagtta aggaactggg tcaatcactg ccacgcttta
    45361 ccgcacacac ctggtatcgt cagccgagcg aagccgatcg cgctaaaggt cagtttgata
    45421 gcgaaggtct gatggatttg agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt
    45481 tctatctctg cggcccggtt ggcttcatgc agtttgccgc gaaacagtta gtggatttgg
    45541 gcgtgaaaca ggaaaacatt cattacgaat gctttggccc gcataaggtg ctgtgatttg
    45601 atgttgccgg atggacacat ccggcaaccc ttgacgcgtt ttaaattgcc gcgtcgtcct
    45661 cttcaccggt acggatgcga atgacccgtg ccacgtcgaa gacgaagatt ttaccatcac
    45721 cgattttgcc ggtttgcgcc gtgcgaataa tggtatcgac acaggtatcg acaatgtcgt
    45781 ccggtacgac aatctcaatt ttcactttcg gcagaaaatc caccatatac tccgcaccac
    45841 ggtacagctc ggtatggcct ttctggcggc caaaaccttt cacctcagtg accgtcatgc
    45901 cggtaatacc gacttcagcc agtgcttcgc ggacatcgtc cagcttgaag ggtttaataa
    45961 tcgcatcaat ctttttcatg ctattccttg aaaaggtcgc ctgtcttttg atctgctaaa
    46021 cgtaacacat aacgccaatt cattccttga aatcgtttgc atccagctcg tttcgggaaa
    46081 gcagtttata aaattctgtc cggttgcgcc ccgccattct cgccgcgtgg gtgacgttgc
    46141 ctttggtgat ttgcagcagc ttacgcaaat agttgagttc aaactgatta cgtgcctcaa
    46201 caaaggttgg cagcgccgta ttttcaccct ccagcgcctg ctccaccagc gcatcactaa
    46261 ttaccggaga tgaggtcagc gccacacact gttcaatcac gttgaccaac tggcgcacgt
    46321 tgcccggcca gcttgcagtc atcaggcgtt tcattgcatc ggtggagaac gcgcggacaa
    46381 acggtttatg tcgctctgcc gcctggcgca acaggtgatt tgccagtagc ggaatgtctt
    46441 ctgtgcgctc cgccagtgcc ggaattttca ggctgacaac attgaggcgg taataaagat
    46501 cttcacggaa ttccccacgc gccatcgctt ttggcaggtc gcggtgagtg gcggaaataa
    46561 tccgcacgtc aatatcaata tcgcggttac tgcccagcgg gcgcacttta cgctcctgca
    46621 acacgcgcag cagtttgacc tgcaacggtg caggcatatc gccaatctca tcaagaaata
    46681 gcgtaccgcc ttccgccgcc tggaataaac cttcgcgatt gctgacagcg ccagtaaacg
    46741 cgccacgtgc atgaccaaac agctccgact ccagcaattg ctcgggtaat gcgccacagt
    46801 taatagcaat aaatggcttg ctgttgcgcg ggctggcgtt gtggatagcc tgggcgaaaa
    46861 tctctttccc ggtgccgctc tggccgttaa tcaaaacgct aacgtctgat tgtgccacca
    46921 gccgcgcctg ttccagcaag cgcagcatca gcgggctgcg ggtgacaatc gcctcgcgcc
    46981 aacgttcgtc ggtggctggc gcggattgtt ccagcgcatc gtcaattgcc tgatatagcg
    47041 catctttatc gacaggcttg gtgagaaaac tgaacacgcc ctgctgtgtt gcagcaacgg
    47101 catcaggaat agaaccatgc gcggtaagaa taattactgg cattcccggc tgcactttct
    47161 ggatttcagc aaacagctgc ataccgtcca tttcatccat ccgcaggtcg ctgatgacta
    47221 aatctacttt ttcgcgattc agtacccgca atccttcagc accactttcc gccgtgacca
    47281 cactgtagcc ttcgctggtc agacgcaggc caagcagttt cagcaatccc ggatcgtcat
    47341 cgaccagtaa taaatgcgca ggtttatggc tcatcaggag tgacctcatc ggtggatggc
    47401 gcgggctttt cactctcgtg cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc
    47461 gagagctggc gttcaatatc ggtcaggttt tccagcttgc gagtagtgag ttccagttgc
    47521 tgttgtagaa cgtgatgttg ctggcgcaaa gtatccagct cgctgtcgct ggactgctgg
    47581 agtttgctgt agcgttggcg ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc
    47641 caaagttgat acagtgggcg aacctgtgcc gggatctcgg tacttagcgc atcaatgcgc
    47701 gcgaccagct ggcggcgctc atacggcgta attttggcgt cggcgagcaa aatcccttgt
    47761 ttaaaggtat tttgccagtt gccgtcgtca tattggcggg cttgctgacg cgactgcgca
    47821 ggcattaaac gatcagcaca atccatcgcc cgcagccagt aaagcggatt ggtttcggtt
    47881 gatttacctt gcagcgccca gatgtcgcta cattcagtag aaagatagtc agccagttga
    47941 taaaccggaa ttttttcttc tgctggcgta tcaatggctg gcctattgtg actctgcacg
    48001 caacccagca atgccagaca tggcagccct gccagccaca gccgtcgggg caataatcgt
    48061 tgaaaaatgt gtcgcatatt caccagactt aaagcctatc ccagtgggcg taattgttgc
    48121 aggcagtttg gacatggaca gcgcggagaa accggagcgt acatgtcgta cgtgaagatt
    48181 tcgagcactg cccggggccg aactgacaaa taaaatagcc tgatgggatg ggctgttagc
    48241 attatttcgt gtttttcgac gacggtaatt caatgcggaa acaaacgtct tgcccgctct
    48301 cgtcgaccag atacagttcc ccttgcatac ggcgaataca atccctggca atgcttaatc
    48361 ccagaccgct gcccttcacc gccccttttc gctggtggct tccctgaaaa aagggttcga
    48421 agatcatggc gcgttcctct tgcggaatgg gcgtgcctgt attgatgaca tcaatataaa
    48481 cccgcgcacc atgtaaactg ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca
    48541 ccgcattgga gtaaagatta tccagtacgc tcatcagcag cattggctcc gccaggcaag
    48601 ctgttgcttt gagatcaacg tcggtatgca tcattttagc ccgtgcgggc aggctatgag
    48661 cagaaaccaa tgtctccacc agcggtgcta actcaacatt ctccagttcc accgcactgt
    48721 ccgcctgttt acggttgtaa tcaagcagtt gttcgatcag tttttgcaaa ttgcggctgc
    48781 tgctatcaag aatgctcacc acctcttttt gctctggcgt aagcggcccg acaacctgat
    48841 cagccagtaa ttcagtgccc tcgcgcatac tcgccagtgg cgtttttaat tcatgagata
    48901 aatgtcttaa aaattgatgg cgttgggatt ccagccatga caggcgctca cttaaccaaa
    48961 gaatacgttg cccaaccgag cgtaactcgc tcggtccact gaacgagacg ctattgccca
    49021 gagaacgccc ttcccccagc cggttgatca tgcgctcgat atttttcacc ggaccgataa
    49081 tcatcctcgt gaaaagcagt accatcacca gactcaccag aaatagcacc agcgattgcc
    49141 aaccaaaata ttgcccacgt tcggcgattt cacgctgaag ttgctgccca cgagagaaca
    49201 ccactgtgcg cgtggcctgc accatttcgg tattggcact ggcaaaggct tccagacgcg
    49261 cggcggcagc agcatcggga ccgctgttgt tacactgaag ttgagccaga ttgttcaaat
    49321 cctgacgtaa tgcctggtag agtttatcgt ccggcagcac gcctgcgtgg gcatcgagca
    49381 tttcgctgta acgcttgcgc tggctttgat aaaccttcgc aagcgtaggg tcgtccagca
    49441 cgcagtactg acggtagctg cgctccatct ccagtgccgc gttggtcatc gcttcactgc
    49501 gccgggcatc aataagcgta gtgcggttaa ccagcgccgc ctgatcgcta agtgcattca
    49561 ggctttgcca ggcttgccat gccagcacca acaggggcag cagaatcagc aaaaatgcca
    49621 gcattaccag ttgtcgtaat gagcggggaa aaacgggcca gcgtttcaag gcgttactct
    49681 cgtcagacgc gaatagcctg atgctaaccg aggggaagtt cagatacaac aaagccggga
    49741 attacccggc tttgttatgg aataaggcgg tgcctaactc gacgtttcgc ccgatggttg
    49801 atatagctaa gctgatatca gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat
    49861 tttatgtagc acgtcccgaa ggggctgaca taagtcggtg aatgagccac tgcttactat
    49921 tatgcagtaa ctgtgccaat aaagaaaata gtttggtaac atactgatta tacgctgctt
    49981 tgaggggttt atggcttctc cgctatttga atgtaaatca gccactgtgt cgctaaaaag
    50041 agacaactta agataaactt attagataat attaaaatca atgagttaag tgtcaccaaa
    50101 aagcgacacg gcaaaccacc cattgtcgtg atttaaagac acaaaaaagc tcccgaagtt
    50161 gggagctcat aatagcggtt ggtgcttaat gccggatgcg gcgtgaatgc ctcatccggg
    50221 ctgcaatacc aatgggttga cgacttaccc caactgctta cgtgcattgc ggaaaatgcg
    50281 catccatggg ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa
    50341 aactcgttcc gggtgcggca tcataatggt gactcgacca ctttcagtcg tgaccgccgt
    50401 aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt
    50461 atcgacatag cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc
    50521 acgcacttcc acgcgccctt caccatgaga cacagcaatc ggcatctgcg agcccaccat
    50581 cccctgcaac agcagcgacg ggctttgggt tacttctacc agactgaaac gcgcttcaaa
    50641 gcgatcggag gtgttacgca caaaacgtgg ccacaactcg ctacccggga tcagttcacg
    50701 cagattagac atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacgatg
    50761 gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac aggatagact tcgcccaacc
    50821 ttcaccagcg cccagcacat caccgtagga gaaaccacca cacgcgacca gggcgtggaa
    50881 accctccagg cccgtgcgtc cagccagcag atcgctcata tgcacgtcga tagcatcgaa
    50941 gcctgcacgg tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag
    51001 cacggcaact ttcgggcgca cgccagtggc aataaacggt gctgccacat cttcgttgat
    51061 atcaaacgac agtttcacat tcaggcccgg atcggcgtcg tttgatttcg cctggtgttc
    51121 ctgatcggca cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca
    51181 ccagacacgc agcgtggtgc ggctttcgct aaatacagtc tgcccgttgg cagtaatcac
    51241 aaaacggtca ccggaaaccg cctgcccaac ataatggacg caatcagcaa gcccatgctg
    51301 tgccagtacc gcttcaacgg cttcacgatc agcagctcga acctgaatca ccgcacccag
    51361 ttcttcgtta aacaacgccg ccaggcgatc gtcacccaga gaggcgatat ccgcgtcaat
    51421 gccacaatga ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg
    51481 gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc
    51541 tttcagttgg gcaacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg
    51601 cgccagtgcc gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcaacagtgc
    51661 gttatcttcg gtagaaagct gcggcgtaat ggtgtgacgt acatcttcca cgcgggcaaa
    51721 tgcggaaatc accagcgaca acggcgacgt catttcgcgc tcttcgttac cttcctgcca
    51781 gcgggttttc atcgacatag agtctttacc caccggtatc gtcaggccca gcgccggaca
    51841 aagttcttcg cccacagctt taacggcttc atacaggccc gcatcttcac cagggtggcc
    51901 tgccgccgcc atccagttgg cggaaagttt aatgcgtttg atatcgccaa tttgtgttgc
    51961 ggcgatgttg gttagcgctt caccgactgc cagacgggca gaagcggcga aatccagcag
    52021 cgcaactggc gcacgttcgc caattgccat cgcttcgcca tagtagctgt cgaggctggc
    52081 ggtagtgacc gcgcagttag cgaccggcac ctgccacggc cccaccatct gatcgcgcgc
    52141 caccatgccg gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt
    52201 cggcagatgc agcacgcgtt tcaccgcgtc agcgatggtg atcccttcac gggccagcgc
    52261 gtcgcctttc gctttcagcg tttgcacatc gcgggtcatc ttcggcgttt tgccaagcag
    52321 tacgtccagc ggtagatcaa tcggctgatt atcaaaatga cgatcgtgca gagaaagatg
    52381 cagttcttcg gtcgcttcac caatcaccgc gtacggtgcg cgttcacgct tacacagttc
    52441 gtcaaacagc ggtaactgat cggcagcaac tgccagcacg tagcgttcct gggattcgtt
    52501 acaccagatt tccagcgggc tcatgcctgg ctcgtcgctc agaatatcgc gcagttcgaa
    52561 tttaccgccg cgcccgccgt cgctcaccag ttccggcatg gcgttagaaa gaccgcctgc
    52621 gccaacgtcg tggataaaca ggattgggtt ggcatcacca agctgccagc aacggtcgat
    52681 cacttcctgg cagcgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag
    52741 gtcggcatca gactgaccag atgccatgga agacgccgcg ccgccgccaa gaccgatgtt
    52801 cattgccggg ccgccgagaa cgaccagctt cgcaccaacg ttgatctcgt ctttttgtac
    52861 gtgatcggcg cgaatgttgc cgatcccacc cgccagcatg atcggtttgt gataaccgcg
    52921 cagctcttcg ccattgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag
    52981 tgccgggcga ccaaattcgt tgttaaacgc cgcgccgccc agcgggcctt cggtcatgat
    53041 gtccagcgcg gtgacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcaaa
    53101 gcccggaata cgcaagttgg atacggagaa accaaccaga ccggctttcg gctttgcgcc
    53161 gcgcccggtg gcaccttcat cgcggatttc accgccagaa ccggtcgccg cacccggcca
    53221 cggagaaatc gccgtcgggt ggttgtgggt ttcaactttc atcagaatat gcgccggttc
    53281 ctgatggaaa tcgtagcggc ccgtttcgtg gtcagcaaag tagcgaccca cttcagaacc
    53341 ttccattaca gcggcgttat ctttataagc agagagaacg tggtctggcg tggtttcgaa
    53401 ggtgtttttg atcatcttga acagcgattt cggctgctgt tcaccgtcga taatccagtc
    53461 ggcgttaaaa attttgtgac ggcagtgctc ggagttcgcc tgcgcgaaca tatacagctc
    53521 gatgtcgttc gggttacgac ccagctttgt gaacgcatcc tgcagatagt caatttcatc
    53581 ttccgccagt gccaggccaa gacgcaggtt agcgtcgatt agcgcctgac ggccctgccc
    53641 cagcaaatct acgctggtga ccggagtcgg ttgatggtgt gcaaacaact gctctgcatc
    53701 atctaaagca aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg tcacctgctg
    53761 ccattgttca ttggtcagcg taccggcttc tatatagtaa gccatgccgc gctcaaggcg
    53821 gtttacctgt tgtagaccgc aattatgggc gatatcggtc gctttcgaag accagggaga
    53881 gatggtgcca ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag
    53941 tgccgggcca tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc
    54001 attgaggtca gcaaaatgga catactcggc gtaaatagtg tgaaccggga gcctggcagc
    54061 ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac aatgcaggcg aaccacgcag
    54121 aatttccatc ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg
    54181 gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca
    54241 tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt
    54301 acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg
    54361 ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca
    54421 tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta
    54481 aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc
    54541 atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg
    54601 cgaaacagtt tgccgattac ctcggtgtaa aactgaaagt gaccgtgcgg cagaatatca
    54661 gccagctgtt tgacgacctc gataatggta acgccgacct gctggcggca ggactggtct
    54721 ataacagtga gcgggtaaaa aattatcagc ctggtcctac ctattattcc gtgtcacaac
    54781 aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg acggcggagc
    54841 aactcaccgt tgcaccgggt cacgtggtgg ttaacgatct ccagaccctg aaagacacaa
    54901 aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag
    54961 atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc
    55021 agcgcgttca cccggaactc gccgtagcgc tcgatatcac cgatgaacaa ccggtgacct
    55081 ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg
    55141 aaatgaatga agacggtaca ctggcacgca ttgaagagaa atacctgggg catggcgatg
    55201 attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt
    55261 taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg
    55321 cttatcagga atcgcactgg gatgcgcagg ccacttcacc gacgggtgtg cgcggcatga
    55381 tgatgttaac caaaaacacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac
    55441 agagcatcag cggcggcgtg cgttatttac aggatatgat gagtaaagtg ccggaaagtg
    55501 tgccggagaa cgaacggatc tggtttgccc tcgccgcgta caatatgggc tatgcgcata
    55561 tgctggatgc ccgcgccctg acggcaaaaa ccaaaggtaa tcctgacagt tgggctgacg
    55621 taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct
    55681 acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaaatac cagattagcc
    55741 tggtgggtta tctgcaggag aaagagaagc aggctacaga agcggcgatg caactggcgc
    55801 aggattatcc ggcggtatcg cctacggagc tgggcaaaga gaaatttcct tttctctcgt
    55861 ttctttccca gtcgtcatca aactatttga cccactctcc ctctctgctg ttttccagaa
    55921 aagggagtga agagaaacaa aattaatctg tcgaggattg cgcttttttc tgcgctttaa
    55981 tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcacactca tccgccagta
    56041 ttccttccgt aatttccacc cggtgattca tacccggatg atgcagcaca tccattaaag
    56101 atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt
    56161 ggatcattgc tccggcacac attacacagg gttcaagcgt gacatacaac gtggcgtcga
    56221 tcagacgata attttgcatc accagaccgc cctgccgtag ggccatgatt tctgcatgtg
    56281 cggtgggatc atgtcgacca atcggacggt tccagccttc gccaataaca cgattgttat
    56341 gcactaatac cgcgccgacc ggtacttccc gctcatccca ggcacgcttc gccagcgtca
    56401 ttgcatgacg catccagtat tcgtagctaa attcgacttc agacaaaaag aaaactccgg
    56461 ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga
    56521 gttcaccgcg cggggttacc cgccagcgat gctggcaaaa gtaaagcagc gggttgtcct
    56581 ggttactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg cgctccagtt
    56641 gcgcgacctt ctcatgcccg aggcaacgca tcgtcaatac ccaaccacca tagccacgct
    56701 gaatctgact ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt
    56761 caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt
    56821 aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcacga aaccagcgta
    56881 cgaaatcggc ctgcaacgcc tgcaaacgcg cttcattgtg accaaaagtg catccccaca
    56941 gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca atggctatga
    57001 tcggcaacaa cggcagaaca agtaacgcat tcagcggttg gcgacgcagt aaataacgca
    57061 aaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac
    57121 gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc
    57181 tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc
    57241 taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg
    57301 tgtctcatat tgcctgttgt cgccattatt tcatcgcgag gaaagatatg aacggcttac
    57361 ttcgtatccg tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc
    57421 tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg
    57481 gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg
    57541 cactaaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatctccc tccgtgccca
    57601 ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag
    57661 aaaatactgc cgctatgtac gcaacgttaa acgttaatag tgaagagaaa ctgcatgaat
    57721 gcgtggcaat gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc
    57781 tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc
    57841 gcgatatgca tgcgctactc gctaccgtac aggcgtcgtc ccctgacgat ctgttattag
    57901 ccatttccta caccggtgta cggcgcgagt taaacctggc ggcagatgag atgctgcgag
    57961 tgggcggaaa agtgctggcg attaccggct ttactcccaa tgccctgcaa cagcgtgctt
    58021 ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt
    58081 gtcacgcgca gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg
    58141 aactggcacc agaacgtatt cgtcatagcg aagcgctggt gaaaaaactg gtctgagtaa
    58201 agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgctg
    58261 ttaatcacta aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg
    58321 atttcgatgg gtgaacatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg
    58381 cactacgaga caccaacctg ccagaaggtg tgtccgatcc ccaatactat tgtgaaagat
    58441 ccgcaacatg tcgagacgga agaacagttg tgggataaat ttgtgctgat gcaccacgcg
    58501 gataaaattt aatattctgc tctggaagta gagtgttaat tattttatag agaa
//
LOCUS       DACADA010000009        55128 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.009, whole genome shotgun sequence.
ACCESSION   DACADA010000009 DACADA010000000
VERSION     DACADA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.009"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            330..2579
                     /gene="fhuA"
                     /locus_tag="ISB17_000560"
     CDS             330..2579
                     /gene="fhuA"
                     /locus_tag="ISB17_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAP1978539.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            2631..3428
                     /gene="fhuC"
                     /locus_tag="ISB17_000561"
     CDS             2631..3428
                     /gene="fhuC"
                     /locus_tag="ISB17_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAP1978540.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3428..4318
                     /gene="fhuD"
                     /locus_tag="ISB17_000562"
     CDS             3428..4318
                     /gene="fhuD"
                     /locus_tag="ISB17_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAP1978541.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            4315..6297
                     /gene="fhuB"
                     /locus_tag="ISB17_000563"
     CDS             4315..6297
                     /gene="fhuB"
                     /locus_tag="ISB17_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAP1978542.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6332..7612)
                     /gene="hemL"
                     /locus_tag="ISB17_000564"
     CDS             complement(6332..7612)
                     /gene="hemL"
                     /locus_tag="ISB17_000564"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAP1978543.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7837..9258
                     /gene="clcA"
                     /locus_tag="ISB17_000565"
     CDS             7837..9258
                     /gene="clcA"
                     /locus_tag="ISB17_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAP1978544.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            9282..9347
                     /locus_tag="ISB17_000566"
     CDS             9282..9347
                     /locus_tag="ISB17_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978545.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9340..9684
                     /gene="erpA"
                     /locus_tag="ISB17_000567"
     CDS             9340..9684
                     /gene="erpA"
                     /locus_tag="ISB17_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAP1978546.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9731..10354)
                     /locus_tag="ISB17_000568"
     CDS             complement(9731..10354)
                     /locus_tag="ISB17_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAP1978547.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10392..11192)
                     /gene="btuF"
                     /locus_tag="ISB17_000569"
     CDS             complement(10392..11192)
                     /gene="btuF"
                     /locus_tag="ISB17_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAP1978548.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(11185..11883)
                     /gene="mtnN"
                     /locus_tag="ISB17_000570"
     CDS             complement(11185..11883)
                     /gene="mtnN"
                     /locus_tag="ISB17_000570"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP1978549.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11967..13484
                     /gene="dgt"
                     /locus_tag="ISB17_000571"
     CDS             11967..13484
                     /gene="dgt"
                     /locus_tag="ISB17_000571"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP1978550.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            13614..15038
                     /gene="degP"
                     /locus_tag="ISB17_000572"
     CDS             13614..15038
                     /gene="degP"
                     /locus_tag="ISB17_000572"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAP1978551.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            15193..16350
                     /locus_tag="ISB17_000573"
     CDS             15193..16350
                     /locus_tag="ISB17_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAP1978552.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(16404..16790)
                     /locus_tag="ISB17_000574"
     CDS             complement(16404..16790)
                     /locus_tag="ISB17_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAP1978553.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(17100..17924)
                     /gene="dapD"
                     /locus_tag="ISB17_000575"
     CDS             complement(17100..17924)
                     /gene="dapD"
                     /locus_tag="ISB17_000575"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAP1978554.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(17955..20627)
                     /gene="glnD"
                     /locus_tag="ISB17_000576"
     CDS             complement(17955..20627)
                     /gene="glnD"
                     /locus_tag="ISB17_000576"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAP1978555.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(20689..21483)
                     /gene="map"
                     /locus_tag="ISB17_000577"
     CDS             complement(20689..21483)
                     /gene="map"
                     /locus_tag="ISB17_000577"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP1978556.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            21851..22576
                     /gene="rpsB"
                     /locus_tag="ISB17_000578"
     CDS             21851..22576
                     /gene="rpsB"
                     /locus_tag="ISB17_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP1978557.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            22711..23562
                     /gene="tsf"
                     /locus_tag="ISB17_000579"
     CDS             22711..23562
                     /gene="tsf"
                     /locus_tag="ISB17_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP1978558.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            23709..24434
                     /gene="pyrH"
                     /locus_tag="ISB17_000580"
     CDS             23709..24434
                     /gene="pyrH"
                     /locus_tag="ISB17_000580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP1978559.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            24584..25141
                     /gene="frr"
                     /locus_tag="ISB17_000581"
     CDS             24584..25141
                     /gene="frr"
                     /locus_tag="ISB17_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP1978560.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            25233..26429
                     /gene="ispC"
                     /locus_tag="ISB17_000582"
     CDS             25233..26429
                     /gene="ispC"
                     /locus_tag="ISB17_000582"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAP1978561.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            26618..27376
                     /gene="ispU"
                     /locus_tag="ISB17_000583"
                     /gene_synonym="uppS"
     CDS             26618..27376
                     /gene="ispU"
                     /locus_tag="ISB17_000583"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAP1978562.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            27389..28246
                     /gene="cdsA"
                     /locus_tag="ISB17_000584"
     CDS             27389..28246
                     /gene="cdsA"
                     /locus_tag="ISB17_000584"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP1978563.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            28258..29610
                     /gene="rseP"
                     /locus_tag="ISB17_000585"
     CDS             28258..29610
                     /gene="rseP"
                     /locus_tag="ISB17_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAP1978564.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            29640..32072
                     /gene="bamA"
                     /locus_tag="ISB17_000586"
     CDS             29640..32072
                     /gene="bamA"
                     /locus_tag="ISB17_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAP1978565.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            32194..32679
                     /gene="skp"
                     /locus_tag="ISB17_000587"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             32194..32679
                     /gene="skp"
                     /locus_tag="ISB17_000587"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAP1978566.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            32683..33708
                     /gene="lpxD"
                     /locus_tag="ISB17_000588"
     CDS             32683..33708
                     /gene="lpxD"
                     /locus_tag="ISB17_000588"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAP1978567.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            33813..34268
                     /gene="fabZ"
                     /locus_tag="ISB17_000589"
     CDS             33813..34268
                     /gene="fabZ"
                     /locus_tag="ISB17_000589"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP1978568.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            34272..35060
                     /gene="lpxA"
                     /locus_tag="ISB17_000590"
     CDS             34272..35060
                     /gene="lpxA"
                     /locus_tag="ISB17_000590"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAP1978569.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            35060..36208
                     /gene="lpxB"
                     /locus_tag="ISB17_000591"
     CDS             35060..36208
                     /gene="lpxB"
                     /locus_tag="ISB17_000591"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAP1978570.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            36205..36801
                     /gene="rnhB"
                     /locus_tag="ISB17_000592"
     CDS             36205..36801
                     /gene="rnhB"
                     /locus_tag="ISB17_000592"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP1978571.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            36838..40320
                     /gene="dnaE"
                     /locus_tag="ISB17_000593"
     CDS             36838..40320
                     /gene="dnaE"
                     /locus_tag="ISB17_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP1978572.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            40333..41292
                     /gene="accA"
                     /locus_tag="ISB17_000594"
     CDS             40333..41292
                     /gene="accA"
                     /locus_tag="ISB17_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP1978573.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            41390..43531
                     /gene="ldcC"
                     /locus_tag="ISB17_000595"
     CDS             41390..43531
                     /gene="ldcC"
                     /locus_tag="ISB17_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAP1978574.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            43588..43977
                     /locus_tag="ISB17_000596"
     CDS             43588..43977
                     /locus_tag="ISB17_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1978575.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            44042..45355
                     /gene="tilS"
                     /locus_tag="ISB17_000597"
     CDS             44042..45355
                     /gene="tilS"
                     /locus_tag="ISB17_000597"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP1978576.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(45389..45649)
                     /gene="rof"
                     /locus_tag="ISB17_000598"
     CDS             complement(45389..45649)
                     /gene="rof"
                     /locus_tag="ISB17_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAP1978577.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(45636..45836)
                     /locus_tag="ISB17_000599"
     CDS             complement(45636..45836)
                     /locus_tag="ISB17_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAP1978578.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            46002..46547
                     /locus_tag="ISB17_000600"
     CDS             46002..46547
                     /locus_tag="ISB17_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAP1978579.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            46544..46966
                     /gene="arfB"
                     /locus_tag="ISB17_000601"
     CDS             46544..46966
                     /gene="arfB"
                     /locus_tag="ISB17_000601"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP1978580.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            46980..47690
                     /gene="nlpE"
                     /locus_tag="ISB17_000602"
                     /gene_synonym="cutF"
     CDS             46980..47690
                     /gene="nlpE"
                     /locus_tag="ISB17_000602"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAP1978581.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(47846..48670)
                     /locus_tag="ISB17_000603"
     CDS             complement(47846..48670)
                     /locus_tag="ISB17_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAP1978582.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(48723..50441)
                     /gene="proS"
                     /locus_tag="ISB17_000604"
     CDS             complement(48723..50441)
                     /gene="proS"
                     /locus_tag="ISB17_000604"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP1978583.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(50552..51259)
                     /gene="tsaA"
                     /locus_tag="ISB17_000605"
     CDS             complement(50552..51259)
                     /gene="tsaA"
                     /locus_tag="ISB17_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAP1978584.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(51256..51660)
                     /gene="rcsF"
                     /locus_tag="ISB17_000606"
     CDS             complement(51256..51660)
                     /gene="rcsF"
                     /locus_tag="ISB17_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAP1978585.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(51778..52593)
                     /gene="metQ"
                     /locus_tag="ISB17_000607"
     CDS             complement(51778..52593)
                     /gene="metQ"
                     /locus_tag="ISB17_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAP1978586.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(52633..53286)
                     /gene="metI"
                     /locus_tag="ISB17_000608"
     CDS             complement(52633..53286)
                     /gene="metI"
                     /locus_tag="ISB17_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAP1978587.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(53279..54310)
                     /gene="metN"
                     /locus_tag="ISB17_000609"
     CDS             complement(53279..54310)
                     /gene="metN"
                     /locus_tag="ISB17_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAP1978588.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            54498..55070
                     /gene="gmhB"
                     /locus_tag="ISB17_000610"
     CDS             54498..55070
                     /gene="gmhB"
                     /locus_tag="ISB17_000610"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP1978589.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13036 a        13569 c        15100 g        13423 t
ORIGIN      
        1 cgttaaatca aaatgttact attccgttaa aagcacgcta catcaaaatg ggtagcgtca
       61 aaagtggtcg ggttaattct gttgccacct tcgaagtgta ttaccgataa gcttttccct
      121 ttgccataaa tgttaatttt tggtaatgga atttttatgg tttacggtgc aaagattaac
      181 atttttgatt catctgattg attctcgccc cttcatggac gcacagagcg tatatcactt
      241 gcgaacccgc cagcgtttcg aatattatcg catctttata ataatcattc tcgtttacgt
      301 tatcattcac tttcatcaga gatataccaa tggcgcgttc caaaactgct cagccaaaac
      361 actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg
      421 cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc
      481 aagaaagcgc atgggggcca gctgcaacta ttgcggcgcg acagtccgct accggcacta
      541 aaactgatac gccgattcaa aaagtacctc aatctatttc tgtcgtcacc gctgaagaga
      601 tggcactgca tcagcctaag tcggtgaaag aagcgcttag ctatacccct ggcgttgctg
      661 tgggaacccg tggcgcatct aatacttatg attacctgat catccgcgga tttgcggcag
      721 acggccaaag ccagaacaac tatctgaatg gcctgaagat gcagggcaac ttctataacg
      781 atgcggttat tgacccatat atgctggagc gcgctgaaat catgcgtggc ccggtttccg
      841 tgctgtatgg gaaaagcagc ccaggcggcc tgttgaacat ggtcagcaag cgcccgacca
      901 ctgaaccgtt gaaagaagtt cagtttaagg ccgggactga cagcctattc cagactggtt
      961 ttgacttcag cgatgcgctg gatgatgacg gcgtttactc ttatcgcctg accggccttg
     1021 cgcgttctgc gaatgcccag cagaaggggg ctgaagagca gcgttatgct attgctccag
     1081 cgtttacctg gcgtccgaat gataaaacca atttcacttt cctttcttac ttccagaacg
     1141 agccggaaac cggttattac ggctggttgc cgaaagaggg gactgtcgaa ccgctgccaa
     1201 acggtaagcg tctgccgacc gactttaacg aaggcgcgaa gaacaacacc tattctcgta
     1261 acgagaagat gattggttat agcttcgacc acgaatttaa cgacaccttt actgtgcgtc
     1321 agaacctgcg ctttgcacaa aataaagtct cgcaaaagag cgtatatggc tacggcatgt
     1381 gctcagaccc gctctattct tcaaacccct cttcgagtcc atgtgctaac gtgccgcagt
     1441 ctcaatgggg ccatacactg actcgtcagt acgttatcga taatgagaag ttagaaaact
     1501 tctctgttga tactcagttg caaagcaaat tcgcaaccgg ttcagtggat cacactctgc
     1561 tgactggcgt tgactttatg cgtatgcgta atgacatcga ttcctggttt ggttatgccg
     1621 gttcagttgc accatctgat atctataatt tagaccgtag tgactttgat tttggcgctc
     1681 acccgaatcc gtctggtcca taccgcgttc tgctgaaaca gaaacaaacc ggcctgtatg
     1741 ttcaggatca ggcgcagtgg gataaagtgc tggttacgct tggcggtcgc tatgactggg
     1801 ccgatcagtc atctttcaac cgtgattacg gtaataaatc tgaacgtgac gacaaagagt
     1861 tcacctggcg tggtggtgta aactacctgt tcgacaacgg cgtaacgcct tacttcagct
     1921 acagtgaatc atttgaaccc gcttcacaga ctgatgcgaa cggcgatctg tttgcacctt
     1981 cgaaaggcaa acagtatgaa gtcggcgtga aatatgtgcc agaagatcgc ccaattgtgg
     2041 tgactggcgc actgtatcag cttaccaaaa ctaataacct gatggcggat ccgaacggtt
     2101 cactgttctc ggttgaaggc ggtgagattc gtgcgcgtgg cgtagagctg gaagcgaaag
     2161 cggcactgtc ggcgagtgtt aacttggttg gttcttacac ctatactgac gcggaataca
     2221 ccactgacac caactacaaa ggaaatacgc ctgcacaggt gccaaaacac atggcttcgc
     2281 tgtgggctga ctataccttc tttgacggtc cgctttcagg tctgacgctg ggcaccggtg
     2341 gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata
     2401 cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg
     2461 tggcgctgca tgtcaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt
     2521 atggttgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat
     2581 ttctcttttt ggggcacgga tttccgtgcc cgtttcacaa gttggctgtt atgcaggaat
     2641 acacgaatca ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc
     2701 gcacgctttt gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg
     2761 gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct tggtcgtcac cagccaccgt
     2821 cggaagggga gattcttatt gatgcccaac cgctggaaag ctggagcagc aaagcgtttg
     2881 cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg
     2941 aactggtggc gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgttgcag
     3001 atcgcgaaaa ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc
     3061 tggtcgatag cctttctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc
     3121 aggatagccg ctgtctgttg ctcgacgaac cgacttcggc gctggatatc gcccaccagg
     3181 ttgatgtact ggcgctggtg catcgtttaa gtcaggagcg tggcctgacg gtcattgccg
     3241 tgctgcacga tatcaatatg gcggcgcgct actgtgatta tctggtcgcc ctacgcggcg
     3301 gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga
     3361 tttatggcat cccgatgggt attctgccgc atccggcggg tgctgcacct gtgagttttg
     3421 tttattgatg agcggcttac ctcttatttc gcgccgccga ctgttaacgg cgatggcgct
     3481 ttctccgttg ttatggcaga tgaataccgc ccacgcggcg gttattgatc ccaatcgtat
     3541 tgtggcactg gagtggttgc cggtggagtt actgctggcg ctcggcatcg tgccttacgg
     3601 cgtggcggac accatcaact atcgcctgtg ggtcagcgaa ccgccattgc cggactcagt
     3661 gattgacgtt ggtttgcgta cagaacccaa ccttgaactg ctgaccgaaa tgaaaccgtc
     3721 gtttatggtc tggtctgcag gatatggccc ttcaccagaa atgctggcac gtattgcgcc
     3781 ggggcgcgga tttaacttca gcgacggcaa acagccactg gcgatggcgc gtaaatcact
     3841 gacggaaatg gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcacta
     3901 tgaagacttt atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttatt
     3961 gctgacgacg cttatcgatc cgcgccatat gttggtcttc ggtccaaaca gcttgttcca
     4021 ggaaattctc gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg
     4081 cagtaccgcc gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt
     4141 tgatcacgac aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat
     4201 gccgtttgtc cgcgccggac gctttcagcg cgtacctgca gtttggttct atggtgcgac
     4261 gctttctgca atgcactttg tgcgtattct ggataacgcc atcggaggta aagcgtgagt
     4321 aaacgaattg cgctttttcc ggtgttattg ctggcgctgc tggtggttgc tgctgcggcg
     4381 ttgacctgga tgaacttctc gcaggcgctg ccgcgcagcc agtgggcgca ggccgcctgg
     4441 tcgccggata ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg
     4501 gcgatttcgc tgctggtggg cgcgggcctg gggatggtgg gcgtgctgtt tcagcaagtg
     4561 ctgcgtaacc cgctggcgga accgacgacg ttgggtgttg caacaggcgc gcaactgggg
     4621 attaccgtca ccacgctctg ggcgatcccc ggcgcgatgg cgagccagtt tgctgcgctg
     4681 gcaggggctt gtgttgttgg cttaatcgtc tttggcgtcg cgtgggggaa acggctttcg
     4741 ccggtaacgc tgatcctcgc ggggctggta gtgagccttt attgcggcgc aatcaatcag
     4801 ttactggtta tcttccatca tgaccaactg caaagcatgt tcctgtggag caccggaacg
     4861 ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc cgcagctgct gggcggcgtg
     4921 atgctgacgt tattgctact tcgcccgtta actctgatgg ggcttgatga tggcgtggcg
     4981 cgcaatctcg ggctggcctt gtcgctcgct cgtctggcgg cgttgtcgct ggcgattgtc
     5041 atcagtgcgc tgctggtgaa cgcggtgggg attatcggct ttatcggttt gttcgcgcca
     5101 ctgctggcga aaatgctggg ggcgcggcgt ttgttgccac ggttgatgct ggcatcgctg
     5161 attggtgcgt taattctgtg gctttctgat caaatcatcc tctggctgac tcgcgtgtgg
     5221 atggaagtgt ctaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg
     5281 ctgccgcgtt tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg tgtcgcgact
     5341 gaacgccaac acgtgctggc gtttgctctc gcgggcggcg tgctgctgtt gatggctgtg
     5401 gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg
     5461 ctcgaggatt tgatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggtgtc
     5521 atgctggcgg tggcgggctg tattattcag cggctgaccg gaaacccgat ggcaagcccg
     5581 gaagtgctgg ggattagctc cggcgcggcg tttggcgtgg tgttaatgct gtttctggtg
     5641 ccgggtaatg cctttggctg gctgttaccc acaggcagtc tcggggcggc ggtgacgctg
     5701 ttgatcatta tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg
     5761 gggatggcgt taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac
     5821 ccgcgaatgg cgcaagttct gacctggatt tccggttcga cctacaacgc gaccgatgcg
     5881 caggtctggc gtactggaat tgtgatggtg attttgctgg cgattacccc gctgtgccgc
     5941 cgctggctga ccattttacc gctgggtggt gataccgcac gagccgtggg gatggcgctg
     6001 acgccgacgc gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacaatg
     6061 accattggtc cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc
     6121 tttcgacgga cgatgccaca catcgtaatt tcggcgctgg tgggaggatt actgctggtg
     6181 ttcgctgact ggtgtgggcg gatggtattg tttccattcc agatcccagc ggggctgctg
     6241 tcgaccttta tcggcgcgcc atattttatc tatttgttga gaaagcagag ccgttaatag
     6301 gatgcctcgc ccggtgggcg aggcaaaatc atcacaactt cgcaaacacc cgacgtgcag
     6361 catcgatggt gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa
     6421 acgctgacgg tgccagataa acaccttcgt ccagcatcat atggaagaaa cgtttaaagc
     6481 gttccacgtc acaggacatc acatcctggt agcatgtcac ggactcggcg tcggtaaaga
     6541 aaataccgaa catgccgcca acgtggttta ccaccagcgg aattccggct tcttctgccg
     6601 cttcccgcag accttctgcc agacgtgttg tcagctcatc cagcgtctcg tgaacgcccg
     6661 gctgtgcgac ttcattcaga caggcgaaac cagcagccat cgcaatcggg ttaccggaaa
     6721 gcgtacccgc ctgatagacc ggaccggtcg gggccagcgc atccattaca tcacgacgac
     6781 caccgaatgc gcctaccggc attccaccgc cgatgatttt gccgaggcag gtgagatccg
     6841 gttccacacc gtaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt
     6901 catcgatgat cagcaacgcg ccaaattcgt cgcacagcgc gcgcagacct ggcaggaact
     6961 ctggcagcgg tggcacgcag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa
     7021 tctcttgcgg gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg
     7081 ttaaggtatg tttggcgaaa tctgccggaa cacccggcga atttggctgg cctaacgtga
     7141 gtgcgccaga accggctttc accagcaagc agtcagcgtg accgtggtaa caaccttcaa
     7201 atttaataat tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg
     7261 cctcggtgcc ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca
     7321 gttgcgccat tttcacttcc atttcggttg gtgcaccaaa gcttaaacca cgctcggcgg
     7381 cttcaatcac ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg
     7441 aaccgacata atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt
     7501 tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacgc
     7561 cgccagggat cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt
     7621 cctgattcgt agaaaaagtg aatggctgct attctatgtt attcataaca agttaaatac
     7681 tcgtcaaaca tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt
     7741 tgtgcatttg aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc
     7801 gtcatcttat ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta
     7861 gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc
     7921 gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg
     7981 gctgcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcactg
     8041 gtacatactg ctgataatta tccgcttctg ttaaccgtcg cttttctctg ttcggcggtg
     8101 ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc cggaagcagg tggttcgggg
     8161 atcccggaaa ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtgttg
     8221 ccggtgaaat tctttggtgg gctggggaca ctcggcggag gcatggtgtt ggggcgcgaa
     8281 gggccaaccg tgcagatagg cggcaatatc gggcggatgg tactcgatgt tttccgcctg
     8341 aaaggtgacg aagctcgtca tacgctactg gcaaccggtg ctgctgcggg gctggctgcg
     8401 gcctttaacg cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt
     8461 cgctatacgt taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg
     8521 taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg
     8581 cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt
     8641 tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg tggcaatatt
     8701 accaaatggg tactgatggg cggtgcgatt ggcggtttgt gtggattgct ggggtttgtg
     8761 gcaccagcaa cttcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc
     8821 agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt actctgcttc
     8881 tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga
     8941 accgctttcg gaatggttgc cgttgagttg tttccgcaat atcaccttga ggcggggacg
     9001 tttgctattg ccggaatggg ggcgttactg gcggcatcta ttcgcgcgcc gttaacgggg
     9061 atcattctgg ttctggaaat gaccgataac taccagctca ttttgccaat gattattacc
     9121 ggtcttggcg caacactatt agcacaattt accggtggga aaccgctata ctcggcgatt
     9181 cttgcgcgca cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca
     9241 gccagcgaga atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt
     9301 tagaatttgc ccaattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg
     9361 ccgctggagt ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat
     9421 aacccgaatc tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat
     9481 ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt
     9541 ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactatacc
     9601 gaaggtctgg aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt
     9661 tgcggttctt cctttagtat ctaatctgct gttcatgtgc cggataaact atccggcaca
     9721 tctcctctct tcaacgacca ttctcatcca gcgcaaacgt cggtagctta agatgccaac
     9781 gaatagccgc cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca
     9841 gtggtacgga aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag
     9901 ttgcgtagat ttctgtacgt aaaatcatgg ggatttcgcg ggccagtaca tcacgaatga
     9961 ttccgccgcc aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg
    10021 cattaaaggc tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca
    10081 ccggcaacat ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc
    10141 tggtgaccat tgcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat
    10201 ccagcgccat gtcgcgaatt gttccgccgc ctactgcggt aaccacgccc aataccagaa
    10261 caccaaaagg gtccatacgc aattttccgg ctaacaaaac gccagagata gcaaataccg
    10321 ctgtgccgac tatatccagc caatagacga gcattgttca atccccactg agcacctgaa
    10381 aaggtcaggc actaatctac ctgtgaaagc gcattacaga gctgttgtgc agcgaggata
    10441 atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc
    10501 tgttcacccc agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaatc
    10561 gcctgtggcg agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg
    10621 tctttaaaga tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt
    10681 ccactggtaa atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca
    10741 gcatattgcg ctttcaattg cgcgtactga tccagcaggg attgcgcggc ttgttcggcc
    10801 ttatccggtt gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg
    10861 cttgtcgcat cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc
    10921 tcggcattac ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc
    10981 agattcatcc cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg
    11041 gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg
    11101 ggagaaagcg tgatgacgcg cggcgcggca ttgagccaca gcggcgcgag aaaagacagg
    11161 gcgaccagcg ccctgaacag tgacttagcc atgtgccagt ttctgcacca gtgactcaac
    11221 catcaggctg gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg
    11281 ttgatcggcc acgtcggaga tggcgcgtac tacgacaaac gggacgttga aattgtggca
    11341 gacatgggcg attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg
    11401 gattttcgcc agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg
    11461 tacagcgtta agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc
    11521 agctttgaag cctgccgggc agcctggtaa ctgaccgtat tcataaccaa atgccgtgac
    11581 atccgcgtcg tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg
    11641 tgccaggcca ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag
    11701 caaagtggca cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc
    11761 ggttccattc agttggccgg tatagatttc gcaaccgccc agactgatag tttgacggtt
    11821 ttcgattttg tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt
    11881 catagattta ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga
    11941 agcgtatttc tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact
    12001 ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga acatgagatt ctgcggatct
    12061 tcgagagcga tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga
    12121 cccaggtttt tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg
    12181 aagtccagca ggtggggcgt tatatcgcta aagagatttt aagccgcctg aaagagctta
    12241 aattactgga gacgtatggt ctcgatgaac tgaccgggcc ctttgaaagc attgttgaga
    12301 tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcggcga
    12361 taaatgactg gtttcgccag cgtttgtacc cggaagatgc cgaaagccag cctctgactg
    12421 acgatcgctg cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgcgc
    12481 tgcggcgcaa gattcgtcag gacttatgtc attttgaagg taacgcacag ggtattcgtt
    12541 tggtgcatac attgatgcgg atgaatctca cctgggcgca ggttggcggt attttaaaat
    12601 atacccgtcc ggcgtggtgg cgtggtgaaa cgcctgagac acatcactat ttaatgaaaa
    12661 agccgggcta ttatctttcc gaagaagcct atattgcccg gttgcgtaaa gaacttaatt
    12721 tggcgcttta cagtcgtttt ccgttaacgt ggattatgga agctgccgac gacatctcct
    12781 attgtgtggc agaccttgaa gatgcggtag agaaaaggat atttaccgtt gagcagcttt
    12841 atcatcattt gcacgaggcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg
    12901 ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt
    12961 tttttatgta tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat
    13021 ttattgataa tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg
    13081 ccagcgaatg cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta
    13141 gccatccaga tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag
    13201 agatttatcg tcctttatta aacctgccgt tatcagactt tactgaactg gtagaaaaag
    13261 aacgggtgaa acgtttccct attgaaacgc gcttattcca caaactctcg acgcgccatc
    13321 ggctggccta tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat
    13381 gggaatatta ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct
    13441 atgcgtggga tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag
    13501 acgaacaata aatttttacc ttttgcagaa actttagttc ggaacttcgc gctataaaac
    13561 gaatctgaag aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa
    13621 aaaccacatt agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct
    13681 ctgcaacggc ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac
    13741 cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg
    13801 ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct
    13861 gccaggaggg ttctccgttc cagagctctc cgttctgcca ggggggcccg ggcggtaatg
    13921 gcggcggcca gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata
    13981 aaggctatgt cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc
    14041 ttagcgatgg tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg
    14101 cgttgatcca gatccagaac ccgaaaaacc tgaccgcaat taagatggcg gattctgatg
    14161 cgctgcgcgt gggtgattac accgtagcaa ttggtaaccc gttcggcctg ggcgagacag
    14221 taacttccgg gattgtctct gcgctgggac gtagcggcct gaacgctgaa aactacgaaa
    14281 acttcatcca gaccgatgca gcgatcaacc ggggtaactc aggtggtgcg ttggttaacc
    14341 tgaacggcga actgattggt atcaacaccg cgatcctcgc accggacggc ggaaacatcg
    14401 gtatcggttt tgctatcccg agcaacatgg tgaaaaacct gacctcgcag atggtggaat
    14461 acggccaggt gaaacgtggt gagttgggta ttatgggcac tgagctgaac tccgatctgg
    14521 cgaaagcgat gaaagttgac gctcagcgcg gtgctttcgt aagccaggtt ctgccgaatt
    14581 cttccgccgc gaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc
    14641 caatcagcag ctttgccgca ctgcgtgctc aggtgggcac tatgccggta ggtagcaaac
    14701 tgaccctggg cttactgcgc gacgggaagc aggttaacgt gaacctggaa cttcagcaga
    14761 gcagccagaa tcaggttgat tccagcacca tcttcaacgg cattgaaggc gctgagatga
    14821 gtaacaaagg caaagatcag ggcgtggtgg tgaacaacgt gaaaacgggg actccggctg
    14881 cgcagatcgg cctgaagaaa ggtgatgtga ttattggcgc gaaccagcag gcagtgaaaa
    14941 acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc
    15001 agcgcggcga cagcactatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa
    15061 aacgggaagg ggttctcctt acaatctgtg aacttcacca caactccata catcttcatc
    15121 atcctttggg catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat
    15181 aatggaggac ttatggctgg ttggcatctt gataccaaaa tggcgcagga tattgtggca
    15241 cgtaccatgc gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc
    15301 ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag gagcattgct ggtactttca
    15361 caaggacgag tcgtcgatat cgatgacgcg gtggcacgtc atctgcacgg tgtgcggcag
    15421 gggattaatc taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt
    15481 gaaccagaga atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg
    15541 gaacagtcgc ggttgatgca cctgttggcg caggatagcc gtttgcggga agaactggtg
    15601 atgaacctga ttcaggctga ggagaatact cccgcactta ctgaatgggc gcaacggctg
    15661 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    15721 ggtgtggaca gcgcaatggc ggagttacag caactgcaaa atgcgctgac tacgcccgag
    15781 cgtaataatc tagtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    15841 aactcttttg ggcgctggga cgcagaagat catcgtaagc gagttgaaca actgattacc
    15901 cgcatgaaag agtatggcca gctgcgtttt cgcgtttcgc tgggcaacta ttttaccggt
    15961 cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    16021 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    16081 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    16141 atggacaata acggcttgct gcggcgaact ctggcggcgt ggtttcgcca caatgtgcaa
    16201 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ctctggaata tcggcttaat
    16261 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    16321 gtggcgttac aactggatga agagcggtag gtcgattaca aaattacagc cgggtgagac
    16381 tttacccggc ccacagaatg acattattta cgcgtcagtt tttccagatc ggcttcaatc
    16441 tcgctgatct tattggttac tactgactcc aggtgacgta agtcatcgag gatcttacgc
    16501 ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac
    16561 cgtagattcg ggctgatttc ctggacttct ttataaccct gccccacacc atcagcgaca
    16621 accgttttac gctgacgcgg atatttaaac ttcacgctct tggcgaaaaa ctcgcctttg
    16681 tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga
    16741 tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct
    16801 caaaatgagt cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact
    16861 gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta
    16921 cctgctttat tgtgatgata ttccatggga tggattagca catttatctt cattttgcga
    16981 attacatgac agtccgaaac aagatgaaaa cgtatttaaa aaagggggaa aatgagtagg
    17041 acgaattatt ctgagccaga aagcaatcgg gcagcgtcgt gctgcccgtg tacatacttt
    17101 tagtcgatgg tacgcagcag ttcgttaata ccaactttac cgcgagtttt cgcgtcaact
    17161 ttcttaacga taactgcaca gtagagactg tatttgccat ctttcgacgg cagattacct
    17221 gaaacaacca cagaccctgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtca
    17281 taaatacggg tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca
    17341 atcacccctt caaccacttc agagcgtgcg ccgatgaagc agttatcttc aataatggtc
    17401 gggttagcct gcagcggctc cagcacgccg ccgataccca cgccaccgga aaggtggacg
    17461 tttttaccaa tctgcgcaca ggaaccgacg gtcgcccagg tatcaaccat ggtgccttca
    17521 tcaacatatg cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaatgaac
    17581 gcgccctgac gcactgccgc tggtggcacg acgcggaagc cttctttctg gaaacgtgct
    17641 tcatcgtagt tagcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg
    17701 atcacctgat tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc
    17761 gtcacccact gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg
    17821 atcacctgat ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca
    17881 cggcgttcaa aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca
    17941 tatcagtaaa cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc
    18001 acttcctgct gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa
    18061 tcttctactc gctcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg
    18121 gcaaaaattt tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg
    18181 aacgatttgc ggtcggtatg ggtcggcaaa aacgtcactt cggtttcaac agtaaaatgg
    18241 cgcaatttag cgggttggcg acggggctgc ggtggctgcc agctactttg tgtcagcacc
    18301 tgctccagac caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt
    18361 tccagcacta taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg
    18421 tgaacactta aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg
    18481 tccgggctcc agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcagtacc
    18541 agcggtttgc ttaaatcatg ctgtaataaa tggcgtgcgt gccaggccag ttgatttggg
    18601 ctatggcgga caaaatagtt agcgcgacag cgtgaccaaa tttggtgcag cgcctcttcg
    18661 tcgatgttat ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg
    18721 cgcatatccg gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt
    18781 tcgcgcaaca gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca
    18841 gccactgtca ggcataccag atagcgcaga cgattttccg tttggacttc ttcggcaaac
    18901 tgcttgatga cttccgggtc ctgaatatcg cggcgctgag ctgtcaccga catcagcaag
    18961 tgctggcgaa ccagccaggc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg
    19021 gcaaaatgca ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg
    19081 atatcgtgaa acagcgcggc gatgaaaatc agctcagttg acggcagacg cggccagacg
    19141 tccacacaca acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcaac
    19201 atcacgcgga tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc
    19261 ccgacgatat gcgaccattg cggcatatac gcgccgagta cgctatggcg atgcattggc
    19321 aatagcccgc gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgcgct
    19381 tccggaatat tacacagagg ttgttgcaga tggcgacggg cgtggcgtaa ctggcgtagc
    19441 gtggtggagt aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag
    19501 atggcttccg gctggcgcat aaatagtgtt tcatcacgca ggtcgatcag cgttccgcgt
    19561 agctgaaact catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct
    19621 tcatcgaaca gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag
    19681 taatccttca tcatccgctc gaccggttcg ttaccttcac cactgtaatt caaacgctgg
    19741 gcgacgctaa gctggcgatc gaataacagg cgattatcgt aacgactgac gaccagatgc
    19801 agggcaaagc gaatgcgcca caatatatgc aggcattcgt ttaattccgc tcgctccgca
    19861 gaggttaaga agccaaaccc taccatttca tccagcgatg ttgcgccaaa atgacggcgg
    19921 gccacccatt gcagagtgtg gatatcgcgc aagccgccgg ggctactttt gatgtctggt
    19981 tcaaggttgt agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc
    20041 gccgcgtaga acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg
    20101 aggaacagcg caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt
    20161 aaatccgata acccttccag catgcactct tcaagcgtgc gcacgctatg acccacttcc
    20221 agctttacat cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg
    20281 agctttttac ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga
    20341 ccgtagccac cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc
    20401 cataatcgtt gaagaagctg gtcgataaac tcggtacgcg cctcaatcaa ctgttctgcg
    20461 gagataccat tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt
    20521 atcccaccga cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgtgc
    20581 agggtgggga gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca
    20641 taagattaaa ttattgcatt aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg
    20701 cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc
    20761 agtcaccaca atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt
    20821 ccagccatct ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt
    20881 gaatgtcatc cccggtttca gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg
    20941 cggttcttcg tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa
    21001 gccttctgct tcgacaaatt tctgaattgc cgcaccgatt tcgcgcagat taatgcctgg
    21061 ttttaccatg cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc
    21121 catgatggtc ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt
    21181 gattacggtg acatcaatgt taacgatatc gccatctttc agcagcttgg cgtcgtccgg
    21241 gataccgtgg cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata
    21301 gccgaggcag gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc
    21361 cagctcgccg gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc
    21421 agccagtcgg ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc
    21481 cattaattct gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg
    21541 gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt
    21601 tttctataat agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc
    21661 cacgacgtat ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact
    21721 ggacggaagc gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg
    21781 gtgccctttg gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga
    21841 ggttttaatc atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg
    21901 tcaccagacc cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa
    21961 agttcacatc atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact
    22021 gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag
    22081 cgaagcggtg aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct
    22141 gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct
    22201 ggaaactcag tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg
    22261 cactcgtgag ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct
    22321 gccggacgct ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa
    22381 caacctgggt attccagtat ttgctatcgt tgataccaac tctgatccgg acggtgttga
    22441 cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt
    22501 tgctgcaacc gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt
    22561 cgtagaagct gagtaataag gcacgctcgt acgagccctt atagaccagg tagtacacgt
    22621 ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc
    22681 cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa
    22741 gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct
    22801 aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg
    22861 aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
    22921 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag
    22981 gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt
    23041 ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc
    23101 aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg
    23161 cgtatcggtg ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct
    23221 atgcacgttg ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg
    23281 gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
    23341 attgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc
    23401 ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac
    23461 gctgaagtga ctggctttat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact
    23521 gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc
    23581 ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg
    23641 ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg
    23701 aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg
    23761 gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgcatgg
    23821 ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg
    23881 gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc
    23941 acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg
    24001 cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca
    24061 gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta
    24121 caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag
    24181 ccgatgtggt gctgaaagca accaaagttg acggcgtgtt tactgctgat ccggcgaaag
    24241 atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga
    24301 aagtcatgga cctggcggcc ttcacgcttg ctcgtgacca taaattaccg attcgtgttt
    24361 tcaacatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt
    24421 taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg
    24481 ggggatctta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    24541 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    24601 aaaagatgct gaagtacgca tggataaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    24661 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    24721 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    24781 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    24841 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    24901 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    24961 tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    25021 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    25081 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    25141 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    25201 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    25261 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa catttccgcg
    25321 tagttgcgct ggtggccggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    25381 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    25441 agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    25501 cgcttaagga tgttgatcag gtgatggcag ccattgttgg cgctgctgga ctgttaccta
    25561 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    25621 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    25681 atagcgaaca taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    25741 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    25801 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc
    25861 cgaactggtc gatgggacgt aaaatttccg tcgattcggc taccatgatg aacaaaggtc
    25921 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    25981 ttcacccgca gtcagtgatt cactcaatgg tgcgctacca ggacggcagt gttctggcgc
    26041 agctgggtga accggatatg cgtacgccaa ttgctcacac catggcatgg ccgaatcgcg
    26101 tgaactctgg cgtgaagccg cttgattttt gcaaactaag tgcgttgaca tttgccgcac
    26161 cggattatga tcgttatcca tgtctgaaac tggcgatgga ggcgtttgaa caaggccagg
    26221 cagcgacgac agcattgaat gccgcaaatg aaatcaccgt ggctgctttt cttgcgcaac
    26281 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    26341 gcgaaccaca atgtgtggat gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca
    26401 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    26461 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    26521 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    26581 agtaccgtaa accctgtcag ggaataagaa acgcgtgatg ttgtctgcta ctcaaccact
    26641 tagcgaaaaa ttgccagcgc atggctgtcg tcatgttgcg atcattatgg acggcaatgg
    26701 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    26761 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    26821 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    26881 tgtgtgggcg ctagatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    26941 tattggcgat accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    27001 gctaacagcc gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    27061 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    27121 agatcagata gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt
    27181 agatttagta attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat
    27241 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    27301 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    27361 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    27421 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    27481 aacgctggtg gtctgtatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    27541 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    27601 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    27661 ttcgctgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    27721 aatctggcgt aactctaaaa cattgcgcat tatttttggc gtgctaacca ttgttccctt
    27781 cttctggggc atgctggcgt tacgggcctg gcactacgac gagaatcatt acagtggcgc
    27841 aatatggctg ctctatgtca tgatcctggt atggggcgct gactctggcg catatatgtt
    27901 tggcaaattg tttggtaaac ataaactggc accgaaggtt tctccgggta aaacctggca
    27961 aggctttatc ggtggactcg tgaccgcagc ggtaatctca tggggttatg gcatgtgggt
    28021 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    28081 agtgctcggc gatctgaccg aaagtatgtt taagcgtgaa gcgggaatta aggacagcgg
    28141 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    28201 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    28261 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    28321 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    28381 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    28441 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    28501 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    28561 gccgcaggtc cagttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    28621 attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg
    28681 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    28741 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattacg
    28801 gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    28861 tttgagcctg ataaagaaga tccggtatct tcgttgggga ttcgtcctcg tgggccgcaa
    28921 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    28981 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    29041 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag acaggggagt
    29101 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    29161 gtcggtattg agccgaaagt cattcctttg cctgatgagt ataaagttgt acgccagtat
    29221 gggccgttca acgctatcgt cgaagccacg gacaaaacgt ggcagttgat gaagctgacg
    29281 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    29341 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    29401 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    29461 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    29521 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    29581 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    29641 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    29701 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    29761 cggccctcct cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta
    29821 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    29881 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    29941 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    30001 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    30061 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    30121 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    30181 acattgttgg taaccatgct ttcaccaccg atgaactgat ctctcatttc caactgcgtg
    30241 acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    30301 gcgaccttga aaccctgcgc agttactatc tggatcgcgg ttatgcccgt ttcaacatcg
    30361 attctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    30421 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaatctt gccgggcact
    30481 ccgctgaaat tgagcaactg actaagatcg agccgggcga gctgtataac ggcaccaaag
    30541 tgaccaaaat ggaagatgac attaaaaagc ttcttggtcg ctatggttat gcctatccgc
    30601 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    30661 ttgatgcggg taaccgtttc tatgtgcgta agatccgttt tgaaggtaac gatacctcga
    30721 aagatgctgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg gggagcgatc
    30781 tggtcgatca gggtaaggag cgtctgaacc gtctgggctt ctttgaaact gtcgataccg
    30841 atacccaacg tgttccgggg agcccggatc aggttgatgt agtttacaag gtgaaagaac
    30901 gtaacaccgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    30961 aggctggtgt gcaacaggat aactggttag gtacgggtta tgctgttggt atcaacggga
    31021 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    31081 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    31141 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg
    31201 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    31261 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    31321 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    31381 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    31441 ttccgggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    31501 ttgatgacga tcacaaatgg gttgttctgg gtcgtacccg ttggggttat ggtgatggtt
    31561 taggcggcaa agagatgccg ttctatgaga acttctatgc cggtggttcc agcaccgtgc
    31621 gtggcttcca gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta
    31681 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gatctgtgta
    31741 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    31801 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata
    31861 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    31921 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    31981 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    32041 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    32101 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    32161 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    32221 ctcggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg
    32281 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaacgag
    32341 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    32401 aagctgcagt ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct
    32461 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    32521 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    32581 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    32641 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    32701 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    32761 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    32821 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    32881 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    32941 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatt
    33001 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    33061 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    33121 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    33181 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    33241 aacgatcgtg gtaactgggt gaagatccca cagattggtc gggtaattat tggcgatcgc
    33301 gtggagatcg gtgcctgcac tactattgat cgtggcgcgc tggatgacac tgttattggc
    33361 aatggtgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    33421 acggcagttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    33481 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    33541 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    33601 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    33661 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    33721 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    33781 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    33841 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    33901 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    33961 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    34021 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    34081 tgtactactt cgccggtatt gacgaagcgc gctttaagcg cccggtcgtg cctggcgatc
    34141 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    34201 ttgctctggt cgacggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    34261 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    34321 gcgcgtcgat tggcgcgaac gctcacattg gtcctttttg tatcgttgga ccccatgtcg
    34381 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    34441 gccgcgataa tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat
    34501 atgctggcga accgacccgc gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    34561 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    34621 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgc atcctcgcca
    34681 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    34741 ccgcagtcca tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg
    34801 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    34861 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    34921 atgcgtataa gctgatttat cgtagcggta agacgctcga tgaagtgaaa ccggaaattg
    34981 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    35041 cgcgcggtct gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg
    35101 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    35161 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    35221 gtacgaaatg gaagaactgg cagtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    35281 cttactacat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt
    35341 tgttggtatt gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    35401 tatcaaaacc attcattacg tcagtccgtc cgtctgggcg tggcgacaga aacgcgtttt
    35461 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    35521 tgacaaatac aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt
    35581 agatccagat aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct
    35641 ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcctgaa
    35701 aacggcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa
    35761 tgccaaacgc cgtgagcagt ttgaacgcat caaagctgca gtcgcgccag acctgtcggt
    35821 tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc agtgatgcgg cgctactggc
    35881 gtcgggtacg gcagcactgg agtgtatgct ggctaaatgc ccgatggtag tgggatatcg
    35941 catgaagcct ttcaccttct ggctggcgaa gcgactggtg aaaactgatt atgtctcgct
    36001 gccaaatctg ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc
    36061 gcaaaaattg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccatgcgat
    36121 gcacgacacc ttccgcgagc tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    36181 acaagccgtt ctggagttag cacaatgatc gaatttgtct atccgcacac gcagctggtt
    36241 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    36301 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    36361 cgccgtctgg cgctctgtga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    36421 gcggaacctc acgaaatcga cgaactgaat attttacatg cgaccatgct ggcgatgcag
    36481 cgtgccgtcg ctggactgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    36541 ccgaaattac cgatgccttc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc
    36601 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    36661 gttttcccgc aatatggttt tgcccaacac aaagggtatc caaccgcttt tcatctggaa
    36721 aaactggctg aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc
    36781 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    36841 tctgaaccac gtttcgtaca cctgcgggtg cacagtgact actcgatgat cgatggcctg
    36901 gccaaaaccg caccgctggt aaaaaaggcg gcagcgttgg gtatgccagc actggcgatc
    36961 accgatttca ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg
    37021 attaagccca tcgtcggggc ggattttaac gtccagtgcg acctgctggg tgatgagtta
    37081 actcacctga cggtgctggc ggcgaacaat accggctatc agaatctgac gttgctgatc
    37141 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    37201 atcgaattaa acgaagggtt gatccttctt tccggcgggc gcatgggcga cgttggacgc
    37261 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    37321 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat
    37381 ctgcacgctg cggtggaact ggcggaatca cgcggtttgc ccgttgtggc gaccaacgac
    37441 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    37501 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    37561 agcgaagagg agatgtgcga gctttttgcc gacattcccg aagcccttgc caacaccgtt
    37621 gagatagcca aacgttgtaa cgtaactgtg cgtctgggtg aatacttctt gccgcaattc
    37681 ccgaccgggg acatgagtac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa
    37741 gagcgtctgg catttttatt ccctgacgag gaagaacgtc ttaagcgtcg tccggagtat
    37801 gacgaacgtc tggatactga acttcaggtt atcaaccaga tgggcttccc gggttacttc
    37861 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gtgtaccggt agggccaggc
    37921 cgtggttctg gtgcagggtc actggtggcc tacgcgttaa aaatcaccga tctcgatccg
    37981 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    38041 ttcgacgttg acttctgtat ggagaaacgt gaccaggtta tcgaacatgt ggcggacatg
    38101 tacggtcgcg atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg
    38161 atccgcgacg taggccgtgt attggggcat ccgtacggat ttgtcgatcg tatctcgaaa
    38221 ctgatcccgc ccgatccggg tatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    38281 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    38341 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    38401 attactgatt ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt
    38461 gataaaagcg acgttgaata cgccgggctg gtgaagttcg acttcctcgg tttgcgtacg
    38521 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    38581 cccccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    38641 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaaaga cctgatcaag
    38701 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccccggc
    38761 ccgttgcaat cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagagatc
    38821 tcctatccgg acgtgcagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac
    38881 ggcatcatcc tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc
    38941 ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct
    39001 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    39061 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactcg
    39121 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aggcacatta tccggcagag
    39181 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    39241 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    39301 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgcgat caaaggggta
    39361 ggtgaaggcc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    39421 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    39481 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    39541 ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    39601 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    39661 caaccgtggc cggagcaggt ggtgttagat ggggaacgtg aaacgttagg tctgtacctc
    39721 acgggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    39781 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    39841 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    39901 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    39961 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    40021 gggcttaaaa tgactgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    40081 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    40141 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    40201 gcggatgcac gcgcgcgatt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    40261 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    40321 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    40381 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    40441 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    40501 gatctcggtg catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg
    40561 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca
    40621 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    40681 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    40741 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgttt taagatgcct
    40801 atcatcacct ttatcgacac cccgggggca tatccgggcg tgggcgcaga agaacgtggt
    40861 cagtcggaag ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt
    40921 tgtacggtta tcggtgaagg tggttctggt ggtgcgctgg cgattggcgt gggcgataaa
    40981 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    41041 ctgtggaaga gcgctgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    41101 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggtgctcac
    41161 cgtaacccgg aagcgatggc ggcgtctctg aaagcgcagc tgctggcgga tctggccgat
    41221 ctcgacgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    41281 ggctacgcgt aatcggcaaa tgttctgaaa agggtcactt cggtggccct tttttatcgc
    41341 cacggtttga gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat
    41401 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
    41461 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa
    41521 gtttatcgaa cataacccac gaatttgcgg cgtgattttt gactgggatg agtacagtct
    41581 cgatttatgt agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa
    41641 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga
    41701 atatgcgctg gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata
    41761 tcttgataac attacgccgc cgtttacgaa agccttgttt acctacgtca aagagcggaa
    41821 gtacaccttt tgtacgccgg ggcatatggg cggtaccgca tatcaaaaaa gcccggttgg
    41881 ctgtctgttt tatgattttt tcggcgggaa taccctcaag gcggacgtct ctatttcggt
    41941 caccgagctt ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat
    42001 cgcgcggacg tttggcgcgg aacagagtta tatcgttact aatggaacat cgacgtcgaa
    42061 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg
    42121 tcataaatcg ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
    42181 gacgcgtaat gcgttgggga ttctgggtgg gatcccgcgc ggtgaattta ctcgcgacag
    42241 catcgaagag aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgatcac
    42301 caactccacc tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt
    42361 cccgtcgatt cacttcgatt ctgcctgggt gccgtacacc cattttcatc cgatctacca
    42421 gggtaaaagt ggtatgagcg gtgagcgtgt tgcgggaaaa gtgatcttcg aaacgcagtc
    42481 gacccacaaa atgctggcgg cgttgtccca ggcgtcgctg atccacatta aaggcgagta
    42541 tgacgaagag gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc
    42601 cattgttgct tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct
    42661 gattaaccgt tcagtagaac gagcgctgca ttttcgcaaa gaggtccagc ggctgcggga
    42721 agagtctgac agctggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg
    42781 ctggcacgtt gcgcctggcg aacagtggca cggctttagc gatgcggatg ccaatcacat
    42841 gtttctcgat ccggttaaag tcactatttt gacaccggga atggacgagc agggcaatat
    42901 gagcgaggag gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt
    42961 agtagagaaa accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac
    43021 caaagcaatg ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct
    43081 gcggatcaaa aatatgctgc ccgatctcta tgcagaagat cccgatttct accgcaatat
    43141 gcgtattcag gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg
    43201 tttgatgttg cgggcatttg ataccttgcc ggagatgatc atgacgccac atcaggcatg
    43261 gcagagacag attaaaggcg aagtagaaac cattgcgctg gaacaactgg tcggtagagt
    43321 atcggcaaat atgatcctgc cttatccacc cggcgtaccg ctgctgatgc cgggagaaat
    43381 gcttaccgaa gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca
    43441 acattacccc ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta
    43501 ccgcgtacga gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac
    43561 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
    43621 attattgcga cggattatgc gatgagcaaa gctttctact gcgatattct tggtttcacg
    43681 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
    43741 gggcaatatg tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg
    43801 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg atatcgatgc ggcagtggcg
    43861 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa
    43921 cgcttcacgt tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
    43981 ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat
    44041 catgacactc acgctcaata gacaacttct ctcctcacgc cagattctgg tggcctttag
    44101 cggcggcctt gattctactg tcttactgca tcaactggtg cagtggcgga cggaaaatcc
    44161 gggcgtcact ctgcgcgcta tccatgtgca tcacggttta agcgccaatg ctgatgcctg
    44221 ggtgaaacat tgtgaaaaca tctgccaaca gtggcaggtg ccgctggtgg tcgaacgtgt
    44281 gcagcttgcg caagaggggc taggtattga ggcccaggcg cggcaggcac gttatcaggc
    44341 atttgcccgt accttgttgc ccggtgaagt gctggttaca gcgcaacatc tcgacgatca
    44401 atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
    44461 ggcggaagtt tcggagtttg ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg
    44521 gggggaactg gagcagtggg cgcgtcagta tgatttacgc tggattgaag acgaaagtaa
    44581 tcaagacgat tcctacgatc gtaacttctt gcgcctgcgc gtagtgccgt tattgcagca
    44641 gcgttggccg cattttgccg aagcgacggc ccgcagcgcc gcactttgcg ccgaacaaga
    44701 gagcctgctg gatgaactgc tggcggatga tttagcgcac tgccaaacgc aacagggaac
    44761 gttaaagatt gcgccaatgc tggcaatgag cgatgcccgt cgcgcggcga ttatccgccg
    44821 ctggctggca gggcagaatg cacctatgcc ttcccgcgag gcgctggtgc ggatctggca
    44881 ggaagtggcg ctggcgcggg aagatgcttc cccctgttta cgtttgggcg cgtttgaaat
    44941 ccgccgctat cagtcgcaac tgtggtggat taaatccgtt gccgggcaaa gcgaaaccat
    45001 tgtgccgtgg cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagtgtaca
    45061 gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
    45121 caaagcgcca ggactgctgc atattgtcgg gcgcaacggt ggacgtaagc tgaagaaaat
    45181 ctggcaagag ttaggcgttc cgccgtggct acgtgacacc acgccactgc tgttttatgg
    45241 cgaaacgctg attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgtaggtga
    45301 gaatggcgtc agttttgtct ggcagaaaac gcttagtcaa gtgaaagccg gataagacgc
    45361 atcaaacgtc gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt
    45421 ccgggtggct aaagctggta attttatcca gacgcagctc gcgggttgca ccagcggcct
    45481 cgacgaccag atactccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt
    45541 tttcgccatc tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat
    45601 tatcgtaatc atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc
    45661 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
    45721 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
    45781 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
    45841 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
    45901 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
    45961 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
    46021 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc
    46081 tgacgttggc gcgccatcct tcagaaaccc aggagcgtat gatgctgcgc ctgctggcgt
    46141 ggctaaaata tgccgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc
    46201 cggaagcgtg gctgcgtaac gatcatttgg gtattgattt gtggattgag ctggggctgc
    46261 cggatgagcg acggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttgcct
    46321 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg
    46381 ccaatctttc cgtctggtat ctggacgacg agcagctggc gaaagtgagc gcctttgccg
    46441 atcgtaccat gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata
    46501 agaataatct ggaagtgaac ttaaccgtct ggcaacaacc ttcatgattg tgatttcccg
    46561 acatgttgct atccctgatg gcgagtttga gatcaccgcc attcgtgcgc agggcgcggg
    46621 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc
    46681 cagcctgcca gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag
    46741 tgacggagtg attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc
    46801 ggcgctggcc cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgaag
    46861 acctacgcgg cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc
    46921 aagcgtgaag gcgatgcgcg gcaaagtgcg cagcggtcgg gagtaaaaag aaggaatgga
    46981 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctttttact ctgatgggat
    47041 gtaataatcg ggccgaagtc gatacgcttt ccccggcgca ggctgccgaa ctgaaaccga
    47101 tgccgcaaag ttggcgcggc gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc
    47161 tgttcctcga aaaagatgga acctgggtga tgaatgaacg ttatctgggg gcacgtgaag
    47221 agccttcctc tttcgcttcc tacggtacat gggcgcgaac tgccgacaag ctggtattaa
    47281 ccgacagcaa aggtgaaaag tcatattatc gagcgaaagg ggatgcactg gagatgctcg
    47341 atcgtgaagg taatcccatt gaatcgcagt tcaactacac gctggaaccg gcaaaatcga
    47401 gcatgccaat gacgccgatg accctgcgtg gcatgtattt ctatatggct gatgcggcga
    47461 cctttactga ttgcgcgact ggaaaacgtt ttatggtggc gaataacgca gagctggagc
    47521 gtggctacct ggctgcgcgc ggcaacggcg aaaaaccggt gttactgtca gtagaaggtc
    47581 actttacgct tgaggctaat ccggataccg gtgcgccgac caaagtatta gcgcccgata
    47641 cggcaggtaa attttacccg aaccaggatt gcagtagttt ggggcagtaa cccgtcttga
    47701 ggcagaaaca aatgaaatat tgcctcatgc gctgcgctta tcaggcctaa agcatacaat
    47761 gcaatatttt gaattagcac gattttgtag gccggataag gcgttcacgc cgcatccggc
    47821 attttgcgca cgaggctggc ttttatcact gagtcagtcc aacaaagcga cttgctttaa
    47881 tataaatccc tggcttcagg tgcccaatat actgtaactg cgtttctggt ttaaatgcag
    47941 agacatcacc actacgcata tgcatcagat cggcagggct aatccagcct gattgcgcca
    48001 gcgtaagcgg atgcccggct ttggcaaatg catccgtgac aaattccgaa caaaaccacg
    48061 actttttgtc tccttcgcca acgttactta actgcgcttt cgccaggccg ctgacgcact
    48121 gttggcgaaa atcctcggag aacggattca gtgagcacat ctggcgagtc accataaatg
    48181 gaataaattc gacaatgccg cgatagttat aaccgctatc tttaatttta ttggcgaacg
    48241 cggtgatttc tgtggcttgt tgcggggtaa ggtccgggac tcgtaagacg aaaagcttat
    48301 cactgtgctt catggctttt ttaagggaaa caatctggac gccagcgcct gtcgcttcgg
    48361 caacgttatt atttcccaaa tagattgcaa cgtgactcac agaggaagtg ctgaatacgc
    48421 ggataccaaa tgaggttacc ccaaggcttg aggagaaaag caaatcgccg ggtttgagat
    48481 ctggttccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttaa
    48541 cagcccatgt tttcgcctcg gcatttaccg ctgtggctga tgagtctggc tggctgatat
    48601 caacggtaca ggccgaaagt aaaagaaagc aggggagaag caggcggcag tacgcctttg
    48661 gtttatccat tttatacaat ccatgtaaaa aagggccccg aaattcagga ccctttctgg
    48721 catcagcctt taatctgttt caccagataa tcgacgatgt caccagtctt aattaactgt
    48781 ttctcgccgt tacgacgata tttatattcg atatcatcgt tgtcgaggtt acggtcgccc
    48841 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    48901 tctttgcggt catccagcag cacttcgata ccttgtgcgc gcagttcgct gtacagtttc
    48961 tcagcaagtt cctgtacgcg gaaggatttg tgcatgttca ttggcagaat cgccacctgg
    49021 aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc
    49081 gctgctacca cacgcgttac gccgataccg tagcaaccca tcgtcaggat ttggttacgg
    49141 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    49201 atgtgaccga cttcgatacc acgtttgatc agcagcgtac cctgaccatc cgggcttgga
    49261 tcgccagcca ccacgttgcg gatatcagca atttccgggg tagcgacatc gcgatcccag
    49321 ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    49381 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    49441 ccggctttaa ccacggcacg aatttcttct tcggtcgcaa aagtcagcgg gctggcaacc
    49501 tgcggcagtt tttctgcttt aacttcgttc agctcgtggt caccgcgcac cagcagcgca
    49561 accagcggga agctactgcc ttcaacggct ttaaccagca gagtcttaac cgttttctca
    49621 atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc
    49681 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    49741 tcaatgttcg ctgcgtagtc agaagtatcg gagaagacca catcgtcttc accgctctgc
    49801 gccagcacct gaaattcgtg agaggcgcta ccgccgatag aaccggtatc ggcctgtacg
    49861 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    49921 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    49981 tcgcgggaac gcatgacgcc aaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    50041 tgatagaagt tcagcggcag ttgtttgtaa gagctcagct cgttacgaat caggtcagtg
    50101 ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc
    50161 agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga
    50221 accaccggca ttaacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    50281 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    50341 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    50401 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    50461 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc
    50521 aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa
    50581 aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg caaccagacg gcataggttt
    50641 tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga
    50701 taaacagcgt taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg
    50761 cggtaaaaca aaccgccatt tctgccgctg gcgcactttg cgcatagctg gcactggcat
    50821 cgggaagcga ttcagcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga
    50881 ccagatccag actgccgagc ttcagaatca cgctgtcttt atggcaaaca acctctttca
    50941 gctctaccag cgacatacca atcgggttgg ggcgaaaagt ggagcgcgta gcgaaaacac
    51001 ccattctggc attaccgccg agacgaggcg gacgcacagt cggacgccag ccgccttcca
    51061 tcgtttgatg aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg
    51121 cgtcggcctg gttgtaggga gcaatgagat gtagttcgcc gttggcgctt tttaccagac
    51181 ctggctggcg cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct
    51241 cgaactggaa actgctcatt tcgccgtgat gttaagcgca gaaccgatac atacagcctg
    51301 acgatagcaa cctggcgtac cgctggtgac ttcgcagcta tgcagtaata ccgcattggc
    51361 tttcattttc gaggcgttga tttgcatccg cttacgtgcg gttggaatgc tcggcggaga
    51421 gtcctgatta gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt
    51481 gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg
    51541 ttttgcaggc tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct
    51601 gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat
    51661 agtatttcct caatgtatga tcaaaacgtc aatattgaat caggcgcttg taaaaatgac
    51721 aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaatatta
    51781 ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga
    51841 ctgataagcc tgaacgaatt tcttcacgtt ttcggcgtct ttgttgtctt cacgcgttac
    51901 gatcaggttt acgtacgggg actctttatc ttcgacaaag ataccgtctt tcgctggagt
    51961 caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc
    52021 cagagagcgc ggcagctgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc
    52081 aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac
    52141 tttttgcagc agcagcagtg aacgaccaag gttagttggg tcgtttggca cggcaacctg
    52201 cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata
    52261 aacaaatgtg ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag
    52321 gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg
    52381 cagaacatag tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac
    52441 tttctgcgcg acttctgcaa cctgctgttc ggcaccaaca atcacgccga ctttaatgtg
    52501 gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc
    52561 cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca attatttatg
    52621 ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat
    52681 taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc
    52741 gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat
    52801 cgcggaataa ccgaccaggg taatcagggt aatagttgcc gcattcacca gacccggcaa
    52861 cgcttccggt aacagcactt tacggacgat ctgcattggc gtggcaccca ttgcgcggga
    52921 ggcttcaatt aacccggttg ggatctccag aagagcgttc tcgaccatac gggcaataaa
    52981 cggtgctgca ccaacggtta acggaacaat cgctgcctgc aatccaatcg atgtaccgac
    53041 aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa
    53101 aatgttcaca atcgcagaaa tggtacgata caacttcgcg ttagcaataa tttgccccgg
    53161 acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc acaaagccaa aaaaaccgga
    53221 tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc
    53281 agacataacc cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt
    53341 gcgtatcttg ttgtgtgccg tgcatttcag tcagcataat gccgaactta acgccaccgg
    53401 cgtaatccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag
    53461 aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt
    53521 cagtaaatgg ctccgcttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg
    53581 tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg
    53641 tgtcctgctc gatcagttcg ccattgctga tgaccgccac gcaatcacaa atgcgcttca
    53701 caacgtccat ttcgtgagtg atcaacagaa tcgtcaaacc cagacggcgg ttgatgtctt
    53761 tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac
    53821 acagcaatac tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc
    53881 caccggaaag attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca
    53941 attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag
    54001 ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa
    54061 tctggcgacg agctttggtc aactcggatt ctgacagcgt ggtcagttcc tggccatcga
    54121 ccagcacgct accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct
    54181 tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga
    54241 cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa
    54301 gttttatcat tgattattta ttatcgtcat taagttagtc gtggcatttc gaatgcctgg
    54361 aacgggcaac gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg
    54421 tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta
    54481 ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca
    54541 ccattaatgt cgatcacggt tatgtccatg agatcgacaa ctttgaattt atcgacggtg
    54601 ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc
    54661 agtctggcat tgctcgcggt aaatttactg aagcacagtt tgaaacgctg accgagtgga
    54721 tggactggtc gctggcggac cgcgatgtcg atctggatgg tatctattat tgcccgcatc
    54781 atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga ctgccgcaaa ccacatccgg
    54841 ggatgttttt gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg
    54901 gcgataaatt agaagatatg caggcagcgg ctgcggcgag cgtgggaaca aaagtgctgg
    54961 tgcgtacggg taaacctatt acgcctgaag cagaaaacgc ggcggattgg gtgttaaata
    55021 gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgtaa tgattaaaag
    55081 atgagcggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagac
//
LOCUS       DACADA010000010        54091 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.010, whole genome shotgun sequence.
ACCESSION   DACADA010000010 DACADA010000000
VERSION     DACADA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.010"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            215..943
                     /gene="mpaA"
                     /locus_tag="ISB17_000611"
     CDS             215..943
                     /gene="mpaA"
                     /locus_tag="ISB17_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAP1978590.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(918..1883)
                     /gene="ycjG"
                     /locus_tag="ISB17_000612"
     CDS             complement(918..1883)
                     /gene="ycjG"
                     /locus_tag="ISB17_000612"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAP1978591.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2002..2508
                     /gene="tpx"
                     /locus_tag="ISB17_000613"
     CDS             2002..2508
                     /gene="tpx"
                     /locus_tag="ISB17_000613"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAP1978592.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2552..4093)
                     /gene="tyrR"
                     /locus_tag="ISB17_000614"
     CDS             complement(2552..4093)
                     /gene="tyrR"
                     /locus_tag="ISB17_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAP1978593.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4241..5302)
                     /locus_tag="ISB17_000615"
     CDS             complement(4241..5302)
                     /locus_tag="ISB17_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAP1978594.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5299..6696)
                     /locus_tag="ISB17_000616"
     CDS             complement(5299..6696)
                     /locus_tag="ISB17_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAP1978595.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6852..7850
                     /locus_tag="ISB17_000617"
     CDS             6852..7850
                     /locus_tag="ISB17_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP1978596.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(7901..8806)
                     /gene="ompG"
                     /locus_tag="ISB17_000618"
     CDS             complement(7901..8806)
                     /gene="ompG"
                     /locus_tag="ISB17_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAP1978597.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNNWHFNIGAMYEIENVEGYV
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSLGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(8851..9933)
                     /locus_tag="ISB17_000619"
     CDS             complement(8851..9933)
                     /locus_tag="ISB17_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1978598.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     IQKIAKEMIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(9947..10606)
                     /gene="pgmB"
                     /locus_tag="ISB17_000620"
     CDS             complement(9947..10606)
                     /gene="pgmB"
                     /locus_tag="ISB17_000620"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP1978599.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(10603..12870)
                     /gene="ycjT"
                     /locus_tag="ISB17_000621"
     CDS             complement(10603..12870)
                     /gene="ycjT"
                     /locus_tag="ISB17_000621"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAP1978600.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGRDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNERLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(12867..13922)
                     /locus_tag="ISB17_000622"
     CDS             complement(12867..13922)
                     /locus_tag="ISB17_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1978601.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASLWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFAFDTQQLREQVTNGVLGEIYVTTARA
                     LRRSGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(13932..14720)
                     /locus_tag="ISB17_000623"
     CDS             complement(13932..14720)
                     /locus_tag="ISB17_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP1978602.1"
                     /translation="MKIGTQNQAFFPGNILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDHRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            complement(14738..15790)
                     /locus_tag="ISB17_000624"
     CDS             complement(14738..15790)
                     /locus_tag="ISB17_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP1978603.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15821..16663)
                     /locus_tag="ISB17_000625"
     CDS             complement(15821..16663)
                     /locus_tag="ISB17_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP1978604.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16650..17531)
                     /locus_tag="ISB17_000626"
     CDS             complement(16650..17531)
                     /locus_tag="ISB17_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP1978605.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17552..18844)
                     /locus_tag="ISB17_000627"
     CDS             complement(17552..18844)
                     /locus_tag="ISB17_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1978606.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQR"
     gene            complement(18858..20537)
                     /locus_tag="ISB17_000628"
     CDS             complement(18858..20537)
                     /locus_tag="ISB17_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP1978607.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(20748..21062)
                     /gene="pspE"
                     /locus_tag="ISB17_000629"
     CDS             complement(20748..21062)
                     /gene="pspE"
                     /locus_tag="ISB17_000629"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAP1978608.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(21137..21358)
                     /gene="pspD"
                     /locus_tag="ISB17_000630"
     CDS             complement(21137..21358)
                     /gene="pspD"
                     /locus_tag="ISB17_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAP1978609.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(21367..21726)
                     /gene="pspC"
                     /locus_tag="ISB17_000631"
     CDS             complement(21367..21726)
                     /gene="pspC"
                     /locus_tag="ISB17_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAP1978610.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(21726..21950)
                     /gene="pspB"
                     /locus_tag="ISB17_000632"
     CDS             complement(21726..21950)
                     /gene="pspB"
                     /locus_tag="ISB17_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAP1978611.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(22004..22672)
                     /gene="pspA"
                     /locus_tag="ISB17_000633"
     CDS             complement(22004..22672)
                     /gene="pspA"
                     /locus_tag="ISB17_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAP1978612.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            22839..23816
                     /gene="pspF"
                     /locus_tag="ISB17_000634"
     CDS             22839..23816
                     /gene="pspF"
                     /locus_tag="ISB17_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAP1978613.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            23970..24107
                     /locus_tag="ISB17_000635"
                     /pseudo
     CDS             23970..24107
                     /locus_tag="ISB17_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            24241..24486
                     /locus_tag="ISB17_000636"
     CDS             24241..24486
                     /locus_tag="ISB17_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAP1978614.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24799..26442
                     /gene="sapA"
                     /locus_tag="ISB17_000637"
     CDS             24799..26442
                     /gene="sapA"
                     /locus_tag="ISB17_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAP1978615.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            26439..27404
                     /gene="sapB"
                     /locus_tag="ISB17_000638"
     CDS             26439..27404
                     /gene="sapB"
                     /locus_tag="ISB17_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAP1978616.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            27391..28281
                     /gene="sapC"
                     /locus_tag="ISB17_000639"
     CDS             27391..28281
                     /gene="sapC"
                     /locus_tag="ISB17_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAP1978617.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            28281..29273
                     /gene="sapD"
                     /locus_tag="ISB17_000640"
     CDS             28281..29273
                     /gene="sapD"
                     /locus_tag="ISB17_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAP1978618.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     SKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            29275..30081
                     /gene="sapF"
                     /locus_tag="ISB17_000641"
     CDS             29275..30081
                     /gene="sapF"
                     /locus_tag="ISB17_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAP1978619.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(30129..31292)
                     /locus_tag="ISB17_000642"
     CDS             complement(30129..31292)
                     /locus_tag="ISB17_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAP1978620.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(31301..32674)
                     /locus_tag="ISB17_000643"
     CDS             complement(31301..32674)
                     /locus_tag="ISB17_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAP1978621.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(32697..35785)
                     /locus_tag="ISB17_000644"
                     /pseudo
     CDS             complement(32697..35785)
                     /locus_tag="ISB17_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(35785..36906)
                     /locus_tag="ISB17_000645"
     CDS             complement(35785..36906)
                     /locus_tag="ISB17_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAP1978622.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(37055..37621)
                     /locus_tag="ISB17_000646"
     CDS             complement(37055..37621)
                     /locus_tag="ISB17_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1978623.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            38099..38887
                     /gene="fabI"
                     /locus_tag="ISB17_000647"
     CDS             38099..38887
                     /gene="fabI"
                     /locus_tag="ISB17_000647"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP1978624.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            39032..40159
                     /locus_tag="ISB17_000648"
     CDS             39032..40159
                     /locus_tag="ISB17_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAP1978625.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            40227..42161
                     /gene="rnb"
                     /locus_tag="ISB17_000649"
     CDS             40227..42161
                     /gene="rnb"
                     /locus_tag="ISB17_000649"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAP1978626.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(42238..42341)
                     /locus_tag="ISB17_000650"
                     /pseudo
     CDS             complement(42238..42341)
                     /locus_tag="ISB17_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            42395..44380
                     /gene="pdeR"
                     /locus_tag="ISB17_000651"
     CDS             42395..44380
                     /gene="pdeR"
                     /locus_tag="ISB17_000651"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP1978627.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            44528..44701
                     /locus_tag="ISB17_000652"
     CDS             44528..44701
                     /locus_tag="ISB17_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978628.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            44790..45539
                     /locus_tag="ISB17_000653"
     CDS             44790..45539
                     /locus_tag="ISB17_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1978629.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            45808..46026
                     /gene="osmB"
                     /locus_tag="ISB17_000654"
     CDS             45808..46026
                     /gene="osmB"
                     /locus_tag="ISB17_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAP1978630.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(46152..46478)
                     /gene="yciH"
                     /locus_tag="ISB17_000655"
     CDS             complement(46152..46478)
                     /gene="yciH"
                     /locus_tag="ISB17_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAP1978631.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(46478..47215)
                     /gene="pyrF"
                     /locus_tag="ISB17_000656"
     CDS             complement(46478..47215)
                     /gene="pyrF"
                     /locus_tag="ISB17_000656"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP1978632.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRDFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(47407..48576)
                     /gene="lapB"
                     /locus_tag="ISB17_000657"
     CDS             complement(47407..48576)
                     /gene="lapB"
                     /locus_tag="ISB17_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAP1978633.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQLLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(48583..48891)
                     /gene="lapA"
                     /locus_tag="ISB17_000658"
     CDS             complement(48583..48891)
                     /gene="lapA"
                     /locus_tag="ISB17_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAP1978634.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(49040..49804)
                     /gene="pgpB"
                     /locus_tag="ISB17_000659"
     CDS             complement(49040..49804)
                     /gene="pgpB"
                     /locus_tag="ISB17_000659"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAP1978635.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            49974..50564
                     /gene="ribA"
                     /locus_tag="ISB17_000660"
     CDS             49974..50564
                     /gene="ribA"
                     /locus_tag="ISB17_000660"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAP1978636.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(50628..53303)
                     /gene="acnA"
                     /locus_tag="ISB17_000661"
     CDS             complement(50628..53303)
                     /gene="acnA"
                     /locus_tag="ISB17_000661"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAP1978637.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSFKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(53453..53548)
                     /locus_tag="ISB17_000662"
     CDS             complement(53453..53548)
                     /locus_tag="ISB17_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978638.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(53662..53829)
                     /locus_tag="ISB17_000663"
     CDS             complement(53662..53829)
                     /locus_tag="ISB17_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978639.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(53832..53996)
                     /locus_tag="ISB17_000664"
     CDS             complement(53832..53996)
                     /locus_tag="ISB17_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAP1978640.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
BASE COUNT        13254 a        14428 c        13105 g        13304 t
ORIGIN      
        1 cagatgcgat taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta
       61 cgacaattct aatatctgca catcaatgaa aatgcaaaca acttccggca aaaaagtgat
      121 acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa tttgcacaac
      181 actgcctgta atacttcctg aaccagagac gatcatgacc gtaactcgcc cacgcgccga
      241 acgcggcgca tttccgcccg gaacagaaca ttacggacgt tcattattgg gcgcgccgtt
      301 aatctggttt ccggcccctg cagccagtca cgaaagtggt ttgattctgg ctggcactca
      361 cggtgatgaa aactcttcga tcgtcacgct ctcctgtgct ctgcggactt tgacaccttc
      421 tttgcgccgc catcatgtgg tgctgtgtgt gaatcctgac ggctgccagt tagggttacg
      481 ggccaatgct aatggtgtgg atttaaaccg aaactttccg gcagcgaact ggaaggaagg
      541 tgaaacagtt tatcgctgga acagcgccgc tgaagaacgt gatgttgtat tactgacagg
      601 tgacaaacct ggctccgaac ctgaaaccca ggcgctgtgc cagcttatac accgcattca
      661 acctgcctgg gtggtctcat tccacgatcc gctggcctgt attgaagatc ccagacacag
      721 cgaattaggt gaatggctgg cccaggcgtt tgaattgcct ctggtaacca gcgtcggtta
      781 tgaaacgcct ggctctttcg gcagttggtg tgcagatctc aacttgcatt gcatcaccgc
      841 agagtttcca cccatctcct ccgacgaagc cagcgaaaaa tatctctttg cgatggcaaa
      901 cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg tgaactgaag cgccggttcc
      961 acatctaccg ccagccaggt cggtccgtca agatcggcga aactgacctg cggcaccagc
     1021 ggtaaagcgg cgctaatggc acgagaggta cacaacatgc agcccagcat cagacgaaaa
     1081 ccttgtgcac gcgcttcagt cgccagcgcc agcgcttccg tcagaccacc ggttttatca
     1141 agcttaatgt taaccatctc atagcgccct ttcagcgcct tcagattgct acgagtatga
     1201 caactttcat cagcacaaat cggtaacgga tgaataaaat tctccagcgc cgcatcgtcc
     1261 tgcgcaggaa gcggttgttc aagcatcgca acgcctaaat ccgccaatag ctggcaacgc
     1321 gccgccaacc cttctgcacg ccaggattca tttgcatcaa cgatcagcgt cgcatcgggc
     1381 acagctgtgc gaattgccac catccgctca ctgataagat ggttatccag cttcactttc
     1441 agtaatttcg cgcctgcctg ccagagtgtt gatgcactat tggccatctg atcaggcgta
     1501 ccgatgacta tcgtctgtgc cgtggtgact gtctcgggaa gcgttatgcc gatcaaatca
     1561 gccagcgatt gctgctgttt tcgcgccgcc agatcccaca atgcacaatc cagcgcatta
     1621 cgtgctgcgc cggcagggag gattttttgc aactcctccc gtgtcagccc tttctctagt
     1681 tgcggcacga cgctcataat ttgcgccatt accgaggcat cgctttcccc ataacgcgga
     1741 tacggcgtgc attcaccggt gcctttgata ccctcttctt ccagttcaac caccaccacg
     1801 cgcgcttcac tgcgacttcc ccgagcaatc acaaacgggg tatgtaaggg ccaggcctcc
     1861 tcaaatacct taacggttct catctttact ccttgttgtc gcggtggtct gcggattaag
     1921 cgcaaatagc gtttgctgtg ttattgacag ttagcataaa ctaggtgtga cgttaactat
     1981 atgtaaacag gaaagataat catgtcacaa accgttcatt tccagggcaa cccggttaca
     2041 gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga cttttactct cgtggcaaaa
     2101 gatctgtctg acgtcaccct cggtcagttt gcgggtaaac gcaaagtgct gaacattttc
     2161 ccgagtattg ataccggtgt ttgcgccgca tcagtacgta agtttaacca actggcaacc
     2221 gagatcgaca acaccgttgt gctgtgtatc tctgccgatc tgccgttcgc ccagtctcgt
     2281 ttctgcggcg cagaaggtct gaacaacgtt atcaccctct ccactttccg taacgctgaa
     2341 ttcctgcaag cctacggtgt ggcaattgct gatggcccac tgaaaggtct ggcagcgcgt
     2401 gccgttgtgg ttattgatga aaatgacaat gtgattttca gccagctggt ggatgaaatc
     2461 accaccgagc cggattacga agcagctctg gctgtactga aagcataagt taatatgcct
     2521 gatggtattg caccatcagg catattcacg cttactcttc gttcttcttc tgactcagac
     2581 catattcccg caacttattg gcaatcgcgg tatgtgaaac gccgagacgt tttgccagtt
     2641 tgcgcgtgct gggataattg cgataaagct gggttaatac cgagcgttca aaacggctgg
     2701 tgatttcgtc cagcgaacct tccatcgcat cttcgcccac ggctaccgtt gcggcgtcat
     2761 aatccggcaa caaaatatcc tgtggacgca gctcataacc gtccagttgt gtcagtgcac
     2821 gatagatagc gttctttaac tgccgcacat ttcccggcca cgcataacgc gtaagtacag
     2881 tattcagatc agcggccagt ttcggacgcg gcacgccctg ctcgtcggca aagcgggcga
     2941 cgaacagctc ggttaacggc atgatgtcct gcggacagtc acgtagcggc ggcagattga
     3001 gcgtcaacac gttcagacga taatagagat cttcacggaa cacgcctttt tgcaccagtt
     3061 cgaccagatt cttctgcgta gcgcaaatca cccgcacatc gacatgcacc tcatggtctt
     3121 cgccaacccg acggaaggtg ccatcattaa ggaaacgcag taatttcgcc tgcatccgtg
     3181 gtgacatctc acctatttca tccaacagca ccgaaccacc gttcgcctgc tcaaagaacc
     3241 ctttcttccc ttccggagca tgaccaaaca gttcactctc gaccgcatct tccggtatag
     3301 acgcacagtt cagcgccagg taaggtttgc tcgctctggg gcttgcctga tgacaggcgt
     3361 aggcaaagag atcttttccg gtgccggtat cacccgtaat cagcagtggc gcgcttagca
     3421 tcgccagttt ctgcgcctgt tcgacaacat gcttcatttt cgggctgaca gcgacaattt
     3481 gactgaaggc gctgacatcc tgggcggcga cattttgcaa ctgacggccc atacgaatcg
     3541 ttgatcgcag catcaccacc gcaccggtca ggacgtgttg atcattttca tcctgaagat
     3601 aaacaggcgt aatctccatc aggaaattct gcccattaat aacgacatgc tcgttatgcg
     3661 aatcttgcgg ttcgctttcc agccagcgta aaaaattaaa gccgttaatc aattgcgcgg
     3721 cggtatggtt gcgcaggcga tccaattttt gcccaaaaag ctgacagctc gccgggttcg
     3781 ccatatccac tttgcttttc atatcgacag agagcacagg ttcaggcaac gcctccagta
     3841 acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca cgggacagta cgcacatcgg
     3901 taacacctgc aatacggcgt atttcggcca tcagactgct gaaactctca aactccagtt
     3961 cagcaaaatt gaggtagatt cgcccaatgg ggtcaatctc aataccgcgt aaatcaatgc
     4021 ctcttagcac gagtagatcg agtaattcgc gggtcagacc gagtcggtct tcacaaaaga
     4081 cttccagacg catgggaacc ttcacctgaa aaaagaacaa ttaatatgat gatatgacac
     4141 tatttggata gcaggaaggt ttctgtcaac aatcattgac acaaagacgg aaaaacgggc
     4201 acttcggcgt aaagatatcc ggtcagcacc cgatattgca ttatttttcg ctgggcgttt
     4261 tgcctttttg cagagtttct ttcacctgac cgataagctg acgacggaaa tccccgaggc
     4321 gtggcttgtc atcgtcaatc cacggcagcg ggcggcaaag ctccatagct ttaatcccga
     4381 gtcgtgccgt cagaagtcca gcaccaatcc cctgagctgc ccgggtagac aaacgcgcag
     4441 cgagatcttg cgacatccag tccatcccca cttcgcgcac cagttcgctg gctccggcga
     4501 aagcgatatt cagcaatacc agcttaaaca ggcgcaaacg gctgtaatat cccagttcaa
     4561 tgccatacag cgtggcgatg cgattaatca aacgcagatt gcgccaggcg ataaacgcca
     4621 tatccaccag cgccagtggg ctaacggcaa tcattaacgt tgattccgcc gctgagcggc
     4681 tgatttcgcg ccgcgcctgg gcatctaaaa ctggctggac caggtgagca tacaaactga
     4741 ccacttcacg atcgttctgc gtttcatgga ttgaggcata ccagcgctgc agcgccggat
     4801 gtgactgatc gatgcccgcc tgctgcgcca gtttttcgca gaatgcacgg cctttacccg
     4861 tgccgtgact gtgcaacaaa tcgcgcgctt catcgcgttc atgggcgcgc tgtcgcaagc
     4921 gccataagcg ccgccactct gttaccacag aacctacgcc agcgccgata atcaatgccc
     4981 cagcggcaca tccacccagc gccacccagt cctgggtttg ccaggcgttc attgtccact
     5041 gcacgccctg cccgacaacg cttgcgccaa acagagccag cccgcccatc accattttgc
     5101 gccacaggct gcgtttcgga cgtaacgctg cgtccatcac cgcttcaact tgcccctctt
     5161 cttcctgtgc ttcgtcgagc gtggccgggg caaaattttg cgcctgattt tcgtcaaagg
     5221 tctgctgcgc cctgaatttt ggattctgat cgacctccag tggaccgtcg aaatcaatac
     5281 gtggttttaa cggttcggtc atcgcaattt atctcctatt aaaaattcca gcgcagcatc
     5341 aagacgaata tgcggtagcg gtttgtcgac atccatcacc tgcggacgaa acgcctcaaa
     5401 ctggaagcct tgcttatccc agaacgcctg acctggcaaa cgtgcgggaa cttcgccagg
     5461 ataaacagtg agcggtgcgc catcgctgag tcgattaccg cgcagcgccg ggattttctc
     5521 accgttaaca tcaatcattc cgctggtggt cgcctgaaca gacgccagcc ccaggcagtc
     5581 catactgatc ccttcgaacg ccgcattttg ccatgcatcc tgaatcagtt gttgcagcaa
     5641 tgaaaccata ttggcgtgct gatcgatggt cacatggtcc gctttagtgg cagcaaacaa
     5701 tagcttatcg ataaccggcg aaaacaaacg ccggaacagg gtacgctgcc cgtagtggaa
     5761 actttgcatc agctgcgtca gcgccagacg catatcatta aatgcctgtg gcccactgtt
     5821 gagaggttgc aggcaatcca ccagcacaat ctggcggtca aagcgcagaa aatgattctt
     5881 atagaacccc ttcaccactt tctcgcagta ataattaaac cgctcgcgca acattccggc
     5941 attagtgtgc ttatcggcct gcgccagttt ggactcgccc caggcatcga catccggcca
     6001 cgggaagaat tgcagcgcgg gtgctcctgc catatctccc ggcaagacaa agcgcccagg
     6061 ctgaataaag tgcaacccct gctgtttgca gtgatggaga tactcggtcc aggcggcggc
     6121 aatgtctgcc agccggtttt cgtcggcagg cgctagcggg tccagccctt cgcacatcat
     6181 tcgccatttc gccgaccatt cgccgcgctg accattgagt aagcccgtca tctggcgcga
     6241 ccagcttaaa tagtcctgcg ccagcatcgg caggtcgagc aaccattcgc cggggtaatc
     6301 cacaatctcc agatacagcg tggaggtatc cttaaagtgg cgcagcagcg aatcgttcga
     6361 tttaaaacgt aacgccaggc gaatttcact gacaccgcgc gtaggcgtcg gccaggcggg
     6421 aggatcgcca tataactgcg ccaacccttc atcataggtg aagcgcggaa tgccaaagtc
     6481 acgctgggga atgcgtttta cgcccagcag gcgctcttca cgcaccgcac ttaaaagcgg
     6541 caaacgtgct ccggcatgaa tattgagcaa ctgattaacc atcgcggtga taaacgctgt
     6601 tttgccgctg cggctaagtc cggttacagc gaggcgcaga tgtctgtcga caccccgatt
     6661 caccagcgca ttgagttcat ttttaagtcg cttcattgct gtcctgtgct gctctgaaga
     6721 ccagaaaaaa aatcgttcgt aaggatatga atgtgggggc caggccattg attatcaact
     6781 acccgtgttg tcatcgacgg gcgaactcca taagatgtgc agcattcacg ttcggacgga
     6841 gttcctgacc tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat
     6901 ccaccgtatc acgcgtgctg aataagcaaa ccaatatctc cccggaagcg cgcgaaaaag
     6961 tgttacgggc cattgaagaa ttacagtatc aaccaaacaa gctggcccgc gcgctgacct
     7021 cttcgggttt tgatgccatt atggtgattt ctactcgttc gaccaaaact acggcgggta
     7081 atccattttt ctctgaagtt ttgcatgcca tcaccgccaa agcagaagaa gaaggtttcg
     7141 acgtgatatt acaaacgtcg cacaatccgg cagaagatct gcaaaaatgc gaaagcaaaa
     7201 ttaagcagaa aatgattaaa ggcattatta tgctaagttc gcctgccgat gaatccttct
     7261 ttgcacaact cgacaaatac gagatcccgg tggtggtgat cggtaaagtt gaaggtcaat
     7321 acagccatgt ttattccgtc gataccgata attatggcga cagtattgcg ctgaccaatg
     7381 cgttaattga aagcgggcat aagaatatag cctgcctgca tgcgccgctt gatgtacaag
     7441 tttcagtgga tcgggtaaat ggttataagc aaagtctggc tacgcataat attgcagtgc
     7501 gtgatgaatg gattgttgac ggcggttata cccatgagac agcattaaaa gcagcacggg
     7561 aattattaag ccagtcaccg ttgcctgagg cagtgtttgc tactgacagc ctgaaattaa
     7621 tgagtattta tcgtgcggcg gcagagaaaa acattgctat tccgcagcag ttagcggtgg
     7681 tgggttatag taatgaaacg ctgtcgttta tattaacgcc tgcaccaggc ggcatcgatg
     7741 ttccgacgca ggagttaggt caacaaagct gcgagttatt attccagtta attgccggaa
     7801 aaccgtcacc acaaaatatt accgttgcta cgcatatgtc gttgaaataa atgtatgccg
     7861 gatttggcgc tcgccccgca tccggcaatt tagcacatta tcagaacgag taattcaccc
     7921 caacacccgc ataatggaat ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg
     7981 cgtattccag cgaaacggaa agaccattct ggaaatcata accgtaaaat aaacccacac
     8041 ggttaaaatc atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat
     8101 ccagcccaat gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg
     8161 cgcgaatttc ttgcgtggaa aactcaccgt tattgcggct atcatccata ttgaaaccac
     8221 gctcgagata atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacccg
     8281 tttccgtttc gacacgggta tcagcataac cggtcgtatt cagatcgttg gcaaatttat
     8341 acatcgacag ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg
     8401 cgattttcca gcgctgcata tttgccgtgt ctttacccgg ttcatcaacg tagtgataac
     8461 catagttacg gaaaccacca gtcagaccga gactgaaatc atcgctttcg aggaactgat
     8521 aatgtacctc cagctccggg cgatcaaacc acgttccacg tttacccgcg ctataatcta
     8581 ccggcccttc ctgataatag gccagagaaa ttctccacgg cccgttggcg gcattaaaat
     8641 agacagaagg ctctgccagc ccatccatat cttcgacata accctcgacg ttttctattt
     8701 cgtacatcgc gccgatatta aagtgccagt tgttcttttc ctcggcctgt gcgcaggcca
     8761 ttcccgcaca catcaccagt gcggtacagg gtaataactt tttcattatt gttccttgta
     8821 ttatcctggg agaatgcctt ccccctgtat ttagcaaatt gctatttccg tttctgcatc
     8881 aaagaaatga catttagtca tatcaaaatg aatagtgata ttttctcctg catgataatc
     8941 atttaacgca ccagcacgga ccactaactc gtgcccccca accgtggtgt agagcataaa
     9001 ttccgcaccg gttaattccg ccacgctaat tttggcggaa atgttatttt cttcttgcgc
     9061 atccggatga atatcctccg ggcgtattcc catcacaatg ggtttatgta aactttcctg
     9121 cgttttcaga accgctaatt tctcttcggg aatggttaat ttaagtgttt ccgtaacgaa
     9181 tttatcgcca tcgatcgtgc cgcgaataaa attcattgct ggtgacccaa taaatccggc
     9241 aacaaacata ttcgccggtt ggttatagac cgttttcggc gcaccgacct gctgaacaat
     9301 cccatccttc ataatgacaa tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt
     9361 cacgtagatc atggtggtgt tcagtttctg atgcagcttg ctgatctctg cgcgcatttg
     9421 cacacgcagc ttggcatcca ggttagagag cggctcatcc attaaaaaca cgcccgcttc
     9481 gcgcacaatc gcccgcccaa gcgccactcg ctgacgttgc ccgccggaaa gcgcgcccgg
     9541 cttgcgtttc aggtactcac gcaggccgag gatttgcgcc gcccagttaa cccgctcatc
     9601 aatcatctct ttggcgattt tttgtatctt caggccaaac gccatgttgt cgtagaccgt
     9661 catatgcgga tacagcgcgt agttctggaa caccatcgcg atattgcgtg ctttggctgg
     9721 aacgtcattc attcgtttgc cgtcgatcaa cagctcaccg ccgctgatct cctcaagccc
     9781 ggcaatcata cgcagggtgg tcgatttacc gcagcccgac ggaccgacaa acacgatgaa
     9841 ctctttatcg gcaatctcca ggctgaagtc cttcaccaca tgcacctggt tatcgtagat
     9901 cttttgaata tgttgtaacg aaagctgtgc catgttgatt cctttgctat acgttttgcc
     9961 agaaggccga taaccgcggc caggtcaggg agtccgttga gggcaacaat aattgcgccc
    10021 cggttaagcc cgcgccgatc cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc
    10081 ccgcctgcgc atcttcaatg ccgatacatg cctgtggcgg cacgcccagc cctgcacagg
    10141 cggcgagaaa gatttccggg tccggtttcg aatttttgag ttgggaagcg tccgcgcaga
    10201 aggtgaaaaa ctcgcgcagc tccagcgctg ctaaaatcgt cggcgcattc agggagacag
    10261 aagccagccc aactggaatc tgctgtgccc ggagatctac cagcaaattg tgaatgccag
    10321 gcagcacagc attaaccgtc agttcgcgca gtgagtggac gtaaagcaga tttttgcgat
    10381 acgccagttg cgcccgttcc tgtgggttaa aatcgccctc tttacccccg tgctgcagaa
    10441 ttcgccgcag ggactcatcg cggctgatcc cttttaggaa ttcgttaaac tgcgcatcaa
    10501 tgctgatgcc aatttcagcg gcaatctgct gccacgcctg gaaatgcaga tgcgcggtat
    10561 cggtgattac tccatccaga tcgaaaatta ccccttgcag tttcattcac cctcctgatg
    10621 cgtggtagcg gtcccattga agggcaaaat aaaatccccc aaacagaaaa cagattcttc
    10681 agcgacgtaa ataagctgac cgttcaaacg cagtgaaaca ggcgcagaag ttcgaatcgc
    10741 aatacgctgc gcgtcgagag tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga
    10801 caactgttgc cactgctcag gtaacgccgg attgagatgt aattcaccgt cacgcacgct
    10861 caccccggca aaaccctgaa tcgcccccag ccagatagcg ccagttgcgg cagcgtggat
    10921 accgtcatca cagctatgcg gatcagcacc aagatcgatt tcagtcccct cgcgccagaa
    10981 ctgataactt tgggtcagca ggccacagcg tgcggcaaca atgccgtgga ttgctttact
    11041 taatgacgag tcgtgaatag tgcgcggttc ataaaattgc agattggcaa gacacgatgc
    11101 cgctgagaac tgctccggca gcatgtaatt gagcatcacc acatcagctt gtttgaggat
    11161 ctgcatctcg ttcacttctg cgcgtgaata atccagcaga atggtttgct tccccgccgc
    11221 cgctttgtat ttcgccagat taatcaccgg cttagccata aacgaatcat cctgcggcaa
    11281 aacgccgtcg ggctgaattt ctggcagccg cagttctttg aggtacattt ctgcacgatg
    11341 aataaacgcg tcgtcgctac agccaaactg acgagcgata ttcagcgcct gctggacgtt
    11401 gtaatacgcc atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtctgg
    11461 cccaataaca tcatgaattt ccagacgctc gttaaccctc accgcgcggc taatccagaa
    11521 ctttgccgtc tccagaagta gcgccatgcc ttcatgagca atgaaacttt catcccccgt
    11581 ggtccgccag tattgaatca ccgcccaggc gatatcggcg accagatggt gttccgcctg
    11641 cgcagaggcc actttttgcc gcagcccggt acgaatgtta atggcggcaa attccggcgt
    11701 ctcttcttcg ccgctacgcg cgctttccca cggaaacaga gcgccctgcc agccgttgcg
    11761 tcgcgctttc tcctgcgcgc caggcaagtt gtgccagcga taacgcagta aacttcgggc
    11821 aaccgtcgga tcgctaaaca gatggaacgg tagcaaaaat acttctgtat cccagaaaac
    11881 gtggcctttg tagccttcgc cggttaaacc tttcgccgca atgctgctgc gctcatcgtg
    11941 ggcaggcgtc atgatgcgca ggtgataaag cgcataatct aatgcttgct gatcgtgcgc
    12001 atcgcggccg tttaccgtga tacgacgttt ctgccaccat tgacgccagt tttctgttga
    12061 tgctgcaaga agttggtcgt aactctgctg cgcgcacatt tcaagctgac gaagcgacgc
    12121 actgccccat tcgtctaaag cggcttgtct gtcgtcccgc cagtcgatcc acaccagttt
    12181 ttgcagcgtc agcgtctcac ctgcgtgaag ctgcgcgcag gtatgttgca ataaacggcg
    12241 ctctttggcg gtatagcatt gctgcacatc accgctcacc ttacagcaac agctgatggc
    12301 cacatcacta cgcccatcct gggtggtgta gatcccctgc atcagatgct gaccaaacac
    12361 ccgcacctgg atttcgtcaa tatgctggcg accgtggttg gtttgcgtgg cgtcaatgcc
    12421 tgttgaaatc agcactgacg cgtcggcgtc cagtggagta atagtgattt ccagcgcaat
    12481 gagcggcagt tggtctgccg aaacaaagcg acggctggcg atggtgtaac cagtgccgtt
    12541 gctggtacgc cagacaacgt tgcgacgtaa ttcgccactg gcaaagttaa gctcacgctg
    12601 ccatgcttcg cgggataacg agaaaatctc accatttatg gcaatctcca tccccacgac
    12661 atcaggcagg ttcaccagtt cgttgatttc tcctttaccc gctcgatgat acagccccgc
    12721 cagatacatc ccacgggtct ggcgcgtgta atcttcttca tggctggcac gaagcccaag
    12781 atagccgttc ccctgcgcca tcagcgaagc atacttgttc agggtatgct ggctgaaatg
    12841 gggttctgat aacgttactg gcctggtcat aattccacac gcgtccctgt ttccgcggat
    12901 tgatacaacg ccgccaccag ttgctggatg atgtacccct gctcggcgtc ggctatcatc
    12961 acgggcttgc cctgtacgtg attgataaag gcttccatgc tgcgcaaatg gcggttgtcg
    13021 tctgccattt cccgttgcat cagcgtcatt aattcaccgt tgttatcggt gtagatatgt
    13081 gccggaaaca gcgtcgcacc ggctttatca ccacaaaagc tgacattcat aatcgactgt
    13141 tcgcggatgt tgagtgcaaa tgacgtttcc agccacagaa tgccgccgtt atgaaattca
    13201 atggtgccaa acagcgaatc ttcaacgctg taagttgccg ggtcccactc gccaaattga
    13261 ccacagctct tttgcgtgcc gatcttttga aagctatgcg cattcacgct tttcaccgcc
    13321 ggaaagccca gcacatacat cgcagcatcc agcatatgaa tgccgatgtc gatcagcggg
    13381 ccaccaccct gcagttcttt attggtaaag acaccccagc cgggaacgcc gctgcgacgc
    13441 agggcgcggg cggtggtaac gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc
    13501 agctgttgcg tatcgaacgc aaaacgatgg tgaaagtcgt aggccagcac cttgccctgt
    13561 ttgcgcgcgg tatcgcacat ttcccgcgcc tgttctgccg tcatggcggg cggtttttcg
    13621 cacatcacat ggcagcccgc ttcaagcgcc atcagggtat gttcgtaatg aaaacggttc
    13681 ggtgagcaga cgctaaccac atcaggtttc accgccagca gcatggcctg cggatcgtcc
    13741 cacaaggatg cattaccgta tttttctgcc agcgcctgcg cctgggaaag gcggctgtca
    13801 cagacggcca ccagttccag atcgttgcgg gtgcagtagt acgaagcatg aaccttatcc
    13861 gccacctggc ctgcgccgat tatcgcgaca cgcagcggag agcttgtcat tgcacttttc
    13921 acaaagacct cttagcaggt gcgcaaccag gccaacgaat cacggtacgc ctgggcagga
    13981 gcttctgccc ggatacgccc ttcatacacc acataaccct gatagttatc ggcgcgcagc
    14041 tgttcaaaca gcgcgtggaa atcgagggtg ccgctgcccg gctggtaacg atggttatcg
    14101 gcaatatgca catgaccgag caagtcgcgg tggtcatgca gcgcctgcgc caggttatct
    14161 tcttcgatgt tcatgtgata gaaatcgccg ataatctgca catgtttaag atcgttttcg
    14221 acgatgtaac ggcgggcatc ggcgagagta ttgatcatat gatcctgata acggtttaac
    14281 ggttcgagat acaccacggt tccggtacgc gcggcgacct gttccagtac gcgcagggaa
    14341 tcactcacca ttttgcggtc gccgtccagg ctgcgcggcg aggtcatcgg cggtaaacgg
    14401 aaggtaaaca tgccccatgc agcaggaacg acgatacctt taccgcccac ttctgccagc
    14461 gcttcgagaa tgcgttcgat ctgctttaag ccattaagac gacgctcttc gataaaatca
    14521 ccaatccagc cgtcatagcc gccgcaggca gtggtcaccg gtaaaccggt ttctttgatc
    14581 gccgctttga cttcttcgat gttgttgacc agcagtttgc cgtcaatctc aaaaccatcg
    14641 aagcccatct ctttgatgta acgaaatttc tccagaatat ttcccggaaa gaatgcctga
    14701 ttctgtgtgc ctattttcat tttttcattc ctttgaatta aaacgtaacg cccattttga
    14761 tgctctgttc cggatgctga tcgacatact gcatgtagct ttccgggctg ttggcaaagg
    14821 tcactaccgg gtcgattaaa tcttcgcaat tgagataacc gttcatcagc agttcccagc
    14881 aggtttcttc aatacgctta cggctccagc gcggataatc cgggttcggt tcgctgcacg
    14941 cgcgggagaa aacaattttg gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt
    15001 cagcaaatgg tttggcaaac gcgacatagg agatggtgcc gccgtaggcc agaccgcgga
    15061 gcgccgactg cagcgcgtcg gcgtagccgc tggtttcgat aatcacatcg gcaccctgct
    15121 tgccggtcag cgttttgatc tctttaccta cgtcagcgcc aatggggtta agacagaaat
    15181 ccgcaccgtg gcgacgggca atatcacagc gatgagcgat aggatcgaca ccaatcacca
    15241 ccgacgcgcc agcgcgtttt gccagttgaa tggcaatttg accaatcgcg ccaagcccta
    15301 ccaccaccac aaaatcgccc acgcgcacgt tggcatcgcg cacgccgctc atggcaaact
    15361 gcgccgggtc gtaacagacg gcgtttttcc aggagctgcc ttgcggcatt ttgcgcagct
    15421 tgtagttatt cactgcgtta atgatgaccg tctcggagag cgggccgtag ccgcatacgc
    15481 tgtcgcccac cgcgtagtcg ttaacgtcgc tgccgcactc gataatgtcg ccaaccacca
    15541 tgttgccaag ctggaacttg ccaaactcaa tgccgcgcgg cgcatttgcc ggacgcgggc
    15601 tgaacatctg ccattcaccg ttaaagtctt catcgataaa cggactggca gcgcggaaat
    15661 cgaccacttc cgtcccgtgc ttcggagcgc cgaaacgcac gcggatcttc acttcattgg
    15721 ctaaaatcgc ccgctcttca tactcaacca gcgcagctac acgcggtgct gtggctacta
    15781 actttttcat gtaaaactcc ttgtaaagaa attggtcgct ttagcccttc acgccaccag
    15841 cggtcaaacc actcttaata aaacgttccg aaagcgcata cataatcact accggtagtg
    15901 cggtcaccag cgaggccgcc atcatccgtc cccagatgta atcaggcgta ctgaacagcg
    15961 cgttcaggcc cacaggtaag gtgaaattgc tggcgctgga aaggaaaatc gacgcaaaca
    16021 gatagtcgtt ccacgccacc ataaagcagt agacgaatac ggatatcagc ccggacattg
    16081 ccaggggcac ggtgatacgg aagataattt gcagacgatt gaggccatcc atcattgccg
    16141 cttcttcgat ttcatcgggg atggtgtcga agtagctttt cagcataaac acggcggtag
    16201 gcagagtttg cgtcaccatg gtgataatca gcgccatctc ggtgtcgtaa atgccaagtg
    16261 cggtgatgat tttgaacagc ggcaccacca gcaaaatgcc ggagaacatg tacaccgtgt
    16321 aaaagctggc gttgatggtc attcgccctt taaagcgcag gcgggaaagc gcgtaagccc
    16381 caagaatgcc gagaaatacc gccaccacgg atgaaaccac cgacaccacc atgctgttac
    16441 ggaagtagtc gacaaacggg aaaatcaccg gattaaaaat gtcgacgtaa tgctccagcg
    16501 tccactgctg tggcagcaac gtaggatgca gcgagatcgc ctcttttgcg ctcttgaacg
    16561 aggtcatcag catcacaaaa aatggaaaca gcgtgatgat gagaaacagc gccagcccgc
    16621 agtaaaaacc gattcgactg agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc
    16681 ctggtcagca gaatgacagc gaaaataatg atgaaaagca ccaccgagat cgccgccgct
    16741 ttccctaagt cattaaaggc aaaggcggtt ttgtagagat agacaccgag aatatcgacc
    16801 ttggtcgtca gcagataaac atccgcgaac atgtagaaca tccagatagt gcgcagggtc
    16861 accaccgtcg ccagaaccgg cataatcgcg ggcagcgtga cgatgcgaaa ccgttgccag
    16921 gcattcgcgc catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc
    16981 gccagaaacg agataaaggc atacgggaag tagcgccaga tggcgaacag caccaccagc
    17041 acaaaactac tgcccgggtt atcgaaccac agcggcgcct gctcatagag atgcagaaga
    17101 tcgacgccga ggtagttcac aatgccgtag ccgttgttga acatgtattt ccaggcgaac
    17161 accagcgaaa tagacggcgt tacgtaggaa aggatcacca gcgaacgcgc ggttttgcgc
    17221 aggcggaact cgcggttgaa aaacatcgcc acggccagcc cgagcacggt gctgcccgcc
    17281 accacaagcg cggtatacca gaccgtcatc cacagcgagt gccagaaacc aggatcggag
    17341 agaatgcgca catagttact caccccaaca aacgttgact cgatgttggg attaagcggc
    17401 agacgtaaaa aactgatttc gatattcgac accatcggcc acgccaccag accgcccagc
    17461 agtaataagc tgggcgcgag aagcaacagc gcaaagggca tatcggaacg acctgaaaaa
    17521 agcctgttca ttgcggattc ctgtcctggg tttagcgctg ttcgaccaac tcatccagct
    17581 ttttctggct cgcttgcagc gtgccaggga ggtcggcttt acccacggtg acgttatgca
    17641 ccattgaact caccacacca gaccccgtca catcacccat gcgggtaaag tttttatccc
    17701 ctactgcgcc aaagacctga atatttggca attcgctgat tagctgattc ggcaattctc
    17761 ccagcgcctt aataacgtcg ttgtctttcc aggtggcggt agtgacaacc gctttgttca
    17821 ccggcagcgc cgcgcctggt gacatcatca cccagtcggc aatgttgtcc gcctgctcca
    17881 taaaggtgac aaatttttca gccgcttcgg tctcttcagt cttttgcccg gcggtaatgg
    17941 tcagcgaggt caacatgccg tagaccgcag aatttttctc ggttggcacc acgaaaccaa
    18001 cgtttttcgg atcgccttct ttaatcacag ccggaaggat ataggtggag taaatcgcca
    18061 tcggcgcggt gccgttcata aaagcgtctt tcacttccat gatgtcgtta gaacctggca
    18121 tggtgttggc agcaaggttg cggtaatagg tcagcgcctg catcatctct ggtgtatcaa
    18181 gggtgatttt gccttcggcg ttaaagacgt tagcctgatt ggataacgca aactgggaga
    18241 aagactgttc cgtcaacacg ctttctgctg taggcagcgc aataccgtat tttttattcg
    18301 ccgggtcatt cagtttctgt gcaacgtcca gcaattgctg ccagtttttt ggctcctcca
    18361 gcccagcttt tgccagcaca tctttgcgat accagatccc gccaatccat gcgctgacag
    18421 gaacaccggt ccaggcgctg ccatcttcgg tgcgcacaat ccgcagtacg ccatcgtaga
    18481 acgcgccttc accgacgttg ctgatgaccg tggcaaccgc tttacgatcg ataagctgct
    18541 ctttgtccat cactttggcg tagtcatggc tggtttcgat cacttccggc agcgaaccgc
    18601 tacgtgaaag ggtaatgact ttggtgttat aggcgtcttc ttccacgggc acttgtttta
    18661 cggtgatacc tgggttttct ttttcaaaac gggcaatcaa tttgctgatg acggcctggc
    18721 gctcctgctc caccgaagaa tgcataaatt cgatggatac attattttta ttttcttctt
    18781 tacagcctga aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa
    18841 tcatgaaatg ttccttttta tttaatccac ataacctgcc agggatgcag tgttatttca
    18901 ttacccaata tatcaacttc actaatcaga tcccgaccgt gcaggtcagt aatatttata
    18961 tgctgaatat tttcgctgac attaaacaat cccgtcaggc aatcgccatc cgcattgctt
    19021 ctttgaattc gcattacaaa tgaattaatt gcatcgatgg tgaaattatt atccggatga
    19081 aactctttat gactgcgacg aagggtaatt aaatgcgaca acttataata taccgaatac
    19141 cttaatgtcg ctttattgtc cagttctgtc gttatctctt tactagagta ttttttacgg
    19201 ttaatcgcgc gattatatcc aagtttttcg acaccggcgt aatcattacg cgaccccaga
    19261 atgctttgaa tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcgaga
    19321 ataaacctgg cacaccgttc ttcgtcgcta cactcacggc ggctcaacgc atccatatag
    19381 gtgacattca tttcataggg gctacgcgta ccgtcgggat tatttttcca gtttactaat
    19441 gccccttcct gctgtaacac ctcgaccagc gctaatattt cgttttcggg taacaacccc
    19501 cgcagtgggt taagcccgat gccatcgtga gaggcaagga agttaaacca ggtggtgttg
    19561 ctggaaggta gtgtcaggtt ttgcgcccac gcacaaagtg cctcaacgtt ctgtttttgc
    19621 accgcatgca gcaccagcgg cggcagcgag aactggtaca ccatatgcgc ttcgtcatcg
    19681 ccttcgccaa agtaagcaat gttgtcttta tgcggaacat tggtctcggt aatgatcact
    19741 gtgcctggcg cgacgttatc aataatcgaa cgtaacagtt tgataatcag atgtgttttt
    19801 tccagatgga tgcagtttgt tcccggctct ttccacataa agccaacggc atccagacgg
    19861 acatactctg cgcccttttc caggtagcac agcaatacat ccaccatcgc cagcaacact
    19921 tcagggctac ggtaattgag gtcaatttga tcgtcgctaa aggtggtcca taaatgacgc
    19981 gttgaatgat cgcgcatctg gaatggcgtt aataacggta acgcacgcgg gcgagtgacg
    20041 gcgctgagat cggttttcgg atcaacggta ataaaaaaat cttcaaaacc tggctgctgt
    20101 tgtaaatagt ttttaaacca ttcacttttt gccgacatat ggttgcagac aaaatcaaac
    20161 attaaatggc tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
    20221 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatggata aaaaggtaat
    20281 aaatgaacat gtgaaaaaat tctttgcagc cattgagggt aaaactgatt aaatgtgggt
    20341 agtggcttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
    20401 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
    20461 acaagtttct gtaaatgagt cgcggtaaat gttccaccgt agatttcgtc caggtaatcc
    20521 gtaattttct gttgcatgtt gcctttccgt ggtagcggtc ccatgaagcg agagactact
    20581 ccgcataaaa taaactgtca attgagctca aggggtttag tcacgcagga aatctttttt
    20641 tacctgggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat gtaaataagg
    20701 tgtgacagaa aaaaacggcg cataagcgcc gctcatggtg aattctttta acctttgacc
    20761 ttcggcattg cgatgtcttt caggccaccg gcgttctcaa cgtgggtata tcccatctcg
    20821 ctaaggatct cttttgcctg ccctgactgg cgtccggcat tgcaatacac tttcacggtg
    20881 tcgtttttat ccggaacggc ggtagcaatg cgctctttca cttccttcag gggaatattg
    20941 atggccccct gaacgtgctc ttgctgatac tgctctggaa cacgaacatc gatccagtgt
    21001 tcagcggcga aaacgggcag tgaaaacacc agtgtcagag caagtaagcc ttttttaaac
    21061 atgtaagcgt ccttctatgg atgaaaattg ctatccatta tacgcctgag atttaaccat
    21121 ttttgcgaaa ctcccctcac cttttataac gctgcgccag tttattggca gcccgactta
    21181 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
    21241 tcgcccagcc cgccacaccc gccgggccat agcgcagtgc ggtaagaagt accagcttgc
    21301 ctgctaattt gaacccaggc ttcacctttt gcccggcctg ttgccagcga gtattcataa
    21361 ctttcctcac agttgacgga aacggctacg cagcgtgaaa gtgtcggaag tgacataacg
    21421 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
    21481 ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
    21541 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga aaatcgacag
    21601 caccaccagg atacgcacca gttttactgg tacatcaaaa tagttggcaa tcccggcgca
    21661 gacgccacgg accatgccct gctgcggaat acgccataat tttttattga gattaatgcc
    21721 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatggattc cagcgcctga
    21781 atacgctcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg ctgctcactt
    21841 tgtgacaatt cactgcgacc ggaacgattg ctgtaatgca gccataacca gatcggtaaa
    21901 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aagtactcct
    21961 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga ttgtcttgct
    22021 tcattttggc ttttaattgt gccagttgct cgctgattgc atcatcggct ttcagttcgg
    22081 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
    22141 tctggtcgat acggcgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
    22201 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ttgatggcgt aacatcaatg
    22261 cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag ctcgccgatc tctttcttca
    22321 tacgcactag cgtatcgtcc accagcgtca cttcatgttc caggctctta atcagatcgg
    22381 tcagtttctg tttttcaatt aacgccgcac gcgccagatc ctctctctct ttcagcagtg
    22441 ccagttccgc tttttcctgc cattcaattt cacgagccga cgcttgttca atacggcgag
    22501 tcagctgttt cttttctgcc agcgcacgcg ccgaagtaga acgtacttca accaatgtat
    22561 cttccatctc ctggatcatc agacgcacca gtttctgtgg atcttccgct ttctctaaca
    22621 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
    22681 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
    22741 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
    22801 gaattagcga attacactaa caagtggcga atttcatcat ggcagaatac aaagataatt
    22861 tactcggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccgc
    22921 tggacaaacc ggtgctcatc atcggcgagc gcggcaccgg taaagagctg attgccagcc
    22981 gtctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
    23041 taaatgaaaa tttgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
    23101 cgcaaaaacg tcatccaggg agatttgaac gtgccgacgg cggtacgcta tttcttgatg
    23161 aactcgctac ggcaccgatg atggtgcagg aaaaattatt gcgtgtgatt gagtacggtg
    23221 aactggagcg cgtcggcggc agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga
    23281 cgaatgccga tctcccggca atggtcaatg aagggacttt tcgcgctgac ctgctcgacc
    23341 gactagcgtt cgatgttgtg caactgccgc cactacgcga acgtgaaagc gacattatgt
    23401 tgatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct ctgttcccgg
    23461 gttttaccga gcacgccaga gaaacattgc tgaattatcg ctggccggga aatatacgtg
    23521 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
    23581 atgacatcat tattgatccc tttaaacgcc gtccatctga agaagccatc gccgtttcag
    23641 aaaacacctc gcttccgacg ctgccgctgg atttacgcga gtttcagatg cagcaggaaa
    23701 aagaactgct tcaggtcagc ttggaacaag ggaagtataa ccagaagcgc gccgctgaat
    23761 tgctggggtt aacctatcat cagttccgcg cgttgctgaa aaagcaccag ctttagcatt
    23821 tcgacaatgc aacttgccgg gtgtgccgtg aacgccttat caggcctaca aaatcaatac
    23881 gtcgtttgca ggcctgataa gcgctgcgca tcaggcatta ttgcgttaac cctgcttctg
    23941 cgtattaatc tctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcaaccg
    24001 ggatggtatg ggcttcgtta ggcggcgcat atttgtggta tttgatccgc cgctatcgca
    24061 aagtgccatt gtatgatggc gacagaacgc cggtgagcga aacgtaatag tcgaatgcgc
    24121 ggcgcattat cctgcctgag ggtaatgcac cgcgccatta actataaaaa tatgtatgac
    24181 ccggatcacg agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata
    24241 atgaaccacg atatcccgct aaaatattat gatattgccg atgagtacgc gaccgaatgc
    24301 gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc actacttcca gttactgctc
    24361 acccgtttga tgaacaatga agagatcagt gaagaagccc aacatgaaat ggctgctgaa
    24421 gcggggatta atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat
    24481 gagtaattat ttaattgcag actatcggtc agtatcgttg cccggataaa gcgttcgcgc
    24541 cgcatccggc taaaaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta
    24601 ccgctctgct cctcgctaag ttgttctgtg agttatagtt ataaagaaat cacgggtacg
    24661 aatgttgaac tgatggcgac gccacctgtt caatgaccaa ccacacggcc ctcattatta
    24721 cctctgaaca caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac
    24781 ataaaatccc ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt
    24841 gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc
    24901 ggttttgtct attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt
    24961 gggttaattg tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc
    25021 tatacctatc gtctgatgcc ggaacttgcc gaaagctggg aagtactcga caacggcgca
    25081 acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc
    25141 actcgtaaaa tgaatgccga cgatgtggtt tttaccttcc agcgaatttt tgaccgcaac
    25201 aacccgtggc ataacgttaa tggcagcaac ttcccctact tcgacagcct gcaatttgcc
    25261 gataacgtga aaagcgtccg caaactggat aatcataccg ttgagttccg tctggctcag
    25321 ccggatgctt cttttttgtg gcacctcgcg acccattatg cttcggtcat gtcggcagaa
    25381 tatgcaggga agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc
    25441 accggtccgt atcagttgtc ggaataccgc gccgggcaat atattcgctt acaacgtcat
    25501 gatgatttct ggcgtggtaa gccgttgatg ccacaagtgg tggtggattt aggctctggc
    25561 ggtacaggac gattgtcgaa actcctgacc ggggagtgcg acgttctggc ctggcctgct
    25621 gccagtcagc tatccatttt gcgtgacgac ccacgcttgc gtttaacgct gcgtcctggg
    25681 atgaacgtcg cctatctggc attcaacacc gccaaaccgc cgctaaataa tcccgctgtc
    25741 cgccatgcgc tggcactggc aattaataac cagcgcctga tgcaatccat ctattatggt
    25801 acggctgaaa cagcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct
    25861 aaaattactg aatacaatcc ggcaaaatcg cgcgaacagt tgaaggcgtt ggggctggaa
    25921 aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg
    25981 aaaaccgccg aactgattca ggcggatatg gcacaggttg gcgtaaaagt ggtgattgtg
    26041 ccggtagaag gtcgttttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta
    26101 tccggttggg cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc
    26161 gcggcaattc attcgcagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg
    26221 ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta ttgaagccta tgacgaagcg
    26281 caaagtattc tggcgcagga attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag
    26341 gcctatcggt acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct
    26401 ggggtgtatc gcgagaaaca ggatgaggtg aaaaagccat gattatcttc accttacgcc
    26461 gtattttgtt attgattgtg accttgttcc tgctgacctt cgttggcttc agcttgagct
    26521 atttcacccc acatgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt
    26581 ttaatggcct gatccactgg gattttggtg tttccagcat taacggtcaa ccgattgccg
    26641 aacagttaaa agaggtcttc ccggcaacga tggaactgtg catcctcgcc ttcggttttg
    26701 cgctgattgt tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg
    26761 ataacttgat caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc
    26821 ttctgttgac gctgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg
    26881 atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg
    26941 actcaccgtg gcgggatgaa atgatcatta gcgcaatccg ccatatggta ttgcccgtga
    27001 tcaccctgtc ggttgcgcca acaactgaag tgatccgact gatgcgtatc agcactatcg
    27061 aagtgtatga ccagaactat gttaaagcag cggcgacccg cggtttgtcg cgctttacta
    27121 ttttgcgtcg ccacgttctg cataacgcgt tacctccggt tattcctcgc ctgggcttac
    27181 agttttccac catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt
    27241 tagggcgctg gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag
    27301 tgatggtgtg tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca
    27361 tggctaaccc tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag
    27421 cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg
    27481 gtcggcctgt acggctgcgc ggggctggct gtactgtgta tttttggcgg ctggtttgcg
    27541 ccttacggca tcgaccagca atttctcggt tatcaattat tgccgccgtc atggtcgcgc
    27601 tatggcgaag tttctttctt cctggggact gacgacctcg gacgcgatgt tttaagccgt
    27661 ttactgagcg gagctgcgcc aaccgtaggt ggcgcatttg tggtgacgct tgccgcgacg
    27721 atctgtggcc tggtactggg caccttcgcc ggggcgaccc acggcctacg ctcagcggtg
    27781 cttaaccata ttctggatac cttactggcg atcccttcgc tgctgctggc aattatcgtt
    27841 gtagcgtttg ccggaccgag tttgtctcac gccatgtttg ctgtctggct ggcgctgctg
    27901 ccacgtatgg tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt
    27961 atcgccgccc gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgccc
    28021 aacatcaccg ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat
    28081 atcgccgcgc tgggctttct cgatctcggc gcacaactgc cctcgcctga atggggagcg
    28141 atgctcggtg atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgcctggt
    28201 gcggcgatta tgattagcgt gctgttggtt aacctccttg gcgacggtgt ccgtcgtgca
    28261 attattgcgg gggtggaata atgccattac tcgatattcg taacctgacc attgaattta
    28321 aaaccggtga tgagtgggtt aaagccgtcg accgcgtaag tatgacctta accgaaggtg
    28381 aaatccgcgg tcttgttggc gaatccggtt ccagtaaaag tttgattgcg aaagcgattt
    28441 gtggggtgaa taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg
    28501 atttgctgcg tctctccgca cgcgaacggc gcaagctggt tggtcacaac gtgtcgatga
    28561 ttttccagga accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc
    28621 aaaacatccc agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac
    28681 gccgtgccat tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca
    28741 gttttcccta tgagttgacc gaaggcgaat gtcagaaagt gatgatagcc attgcactgg
    28801 cgaatcaacc gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc
    28861 aggcgcaaat ctttcgcctc ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc
    28921 ttatcagcca tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact
    28981 gcggtcaaac ggtggaaacc gcgccgagta aggagttggt gacgatgcca caccatcctt
    29041 atacccaggc gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc
    29101 gcctcaatac gctgcccggc gctatcccgc tactggaaca gttaccgatt ggttgtcgtc
    29161 tggggccacg ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg
    29221 cgaaaaatca tctctatgcc tgtcatttcc cactgaacat ggagaaagag tgagatgatc
    29281 gaaacgctac ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggctggttt
    29341 cgtcgtcaga ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca
    29401 ctggcgatta tcggcgagaa tggttcgggt aaatccacgc tggcaaaaat gctggcggga
    29461 atgatagaac ctaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat
    29521 tattccttcc gcagtcagcg tattcgcatg atttttcagg atccttccac ctcgttgaat
    29581 ccccgtcaac gtatctcgca aattctggat tttccactgc gcctgaacac cgacctggaa
    29641 ccggaacagc gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat
    29701 cacgtcagtt actatccgca tatgttggca cccgggcaaa aacagcgtct gggtctggcg
    29761 cgcgcgctga tattgcgccc aaaagtcatt attgccgatg aagccctcgc ctcactggat
    29821 atgtcgatgc gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt
    29881 tcgtatattt atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg
    29941 gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg cagatgtgct ggcatcgccg
    30001 ctgcatgaac tcaccaaacg gctgatcgcc ggtcattttg gcgaggcatt gacggcggat
    30061 gcgtggcgta aggaccgtta gtgttttgca cctcatcctg acaggccccc ctcaggatga
    30121 ggtgttcatt acttttggtt gtcgattctg gtgagagcaa ccagcaatgc cagcgtataa
    30181 cacaccgtaa ttgccagact catttttaac agtgtttctc cgccaattcc cgacaatggc
    30241 gcggctatac cgccgaagac aaacatgctc atccccatca acgcagacgc cgttccagat
    30301 tcctgcgtcc ctactgcact catagcctct gccccgccaa cagtgccgat gccgctacaa
    30361 aatgcgatgg tgaaaaataa tgccaccagt gccggaatgg gcaattgtat ccatgcgcat
    30421 aagaccgtga gcaatgcaca caaaattgcc gctatcaggc cacctcgcaa caatgtcatc
    30481 gcgttaatcc gtcgcgccag gcgcgagaag atccatgaag caatgataag tccggtgccg
    30541 ttaaggccaa acaccaggct aaattgcatt ggactaaagc caaattcctt ctgcaacacg
    30601 aacgacgaag agccgatata tgcaaacaaa ccggccagca taaaactttg aatcaggcaa
    30661 aagcgcataa aacggcggtt ctgcaccacg cttcgtccgg ttaatagcaa tgatgagccc
    30721 cttttatttt ctggcaaggt ctcatgaata aataacagac agcccagcaa cagtacggtg
    30781 ctaatttcag ccattaccca gaataaagtg cgccagtcaa aagtgctaac aatgtacccg
    30841 cccagcactg gcgacaacac cggtgccagg ccattcaccg tcattaacag cgcaaaaaac
    30901 tgggtcaacg ttactccctg atatttgtca cgagcaatag aacgagagag caccgaacca
    30961 cccgcccccg cgatcccttg aataaaacgc cagaccacca gccagtaaat attgttcgtt
    31021 gtcgcgcata aaatggaaga aacaataaac aatagcaacg acaagattaa cggacgttta
    31081 cgccctattt tgtcgctcag agggccaaat aacagttgtc cgacgccaag accaatcagt
    31141 gatgcggtca gggttaattg cgttatggtc gtggttgccg ccagttgctc agacatctcc
    31201 ggcaaagccg gcaaataaag atcggtacac atcgggccga taccggctaa aagaccaaga
    31261 atcaatgccc atgaaagaga gactctagcc atattattcc ttacttgagt gatgacatac
    31321 cgccgcccag cgactgccag agcgtgattc ggtttgtaaa atcagtcagt tgcagtgcca
    31381 gcagctcctg ctgtgcgctc cacaagctgc gctgcgccgt caacacggtc aggtaatcac
    31441 caacacccgc ctgatagcga cgcaagccaa catcgaccgt ttgttgttca gctttaacat
    31501 actggcgctg tgcatccagt tgctcttcaa gcgtggtccg ccgcgccagt gcgttagcga
    31561 catctttaaa tgcgctctga acggtttttt cataggtggc aatgagtcca cgcttttgtg
    31621 cttctgcgta acgaagctgc gccagattac ttccgccagt aaacagcggt aatgtgaccg
    31681 atggcgcaaa tgaccagatc tgcattccgt ggctgaacaa cgacgacaac gcgtcactgc
    31741 caacaccggc actggcagtc agcgaaatcg tcgggaaaaa gttagcccgc gccgcgccaa
    31801 tatctgcatt ggcgctcttc aaattatgtt ccgcttcctg gatatccggg cgacgcagca
    31861 gaacatcaga tgatactccc gcaggaacca gacttatcat ttgttccggc aggctttcca
    31921 gtgttcccgg tagcagattt tccgcaagcg ttgtacctgc cagtaagttc agcgcatttt
    31981 tatcctgcat cacttgtgtc tgatagctgg caacgctggc gcgcgcttgc tgataaacac
    32041 tcatcgcctc actgacatct gtcgctgccg ccgtgccaac ctgttgctgg cgctggataa
    32101 ttttcaatga gttttctgca ctggccatcg tttcttttgc cagggccagg ttgctgttat
    32161 cagccgccag tgtcagccag gcggtgctga tttcagcaat cagcgttaaa cgagtgttct
    32221 gggctgtaaa ttcactggca agccaggttt cccgcgccgc ccgagataaa ctctgattac
    32281 gaccaaacag atctaacgta taactggaca ccgtgccgtc agcctcggca gttgttcccg
    32341 taccattcgc cagcgaacgg ctgcgggtcg aacttaaggc cgcattcacc gtcggaaata
    32401 acgacgcatt agtttgccca tagagcgccc gtgctgagtc aatatcagca atcgcttttt
    32461 gcacatcgcg gttactgttc agtgcaatgg ttactacctg ctgcaatcgg ggatcgtgga
    32521 tcacctgctg ccagtcatgg ctgattgcct tcccctggcc ctgtgctccc ggcaaggtcg
    32581 ccgggattgg ggattcaggc gtactgtagt gaggatcgag cgagacacat cccgcactca
    32641 acaacaccag aaaaataaga gaacgacgca acataactta ctcctgacgg cgcggtttac
    32701 cggcaaacaa acgtttcacc agtacaaaaa acagaggaac gaagaaaatg gccagtaatg
    32761 tagcggtcag cgtgccgcca ataatgcccg taccaatggc gatgcggctg ttcgcccctg
    32821 cgccggttgc tatcgccagc ggcattaccc ccgcaataaa cgccagcgag gtcatgatga
    32881 ttgggcgtaa acgagtctgc gccgcgcgta atgccgcacg actcaaggaa tagccctctg
    32941 caaccgccgc ttcagcaaat tccacaatca gaatggcgtt tttcgacgac aagccgatag
    33001 tagttaatag cgctacctgg aagtaaacgt cgttgtttaa atcacgcatc caggccgcca
    33061 gcgccgcgcc aagaagcccc agagggatca ccagaatgac cgagaacggg actgaccagc
    33121 tttcgtacag tgctgcaagg cagaggaaaa ccaccagaat agaaactgca tacagactta
    33181 atgcctgacc gctggccagt ttctcctgca atgacaaacc actccaggcc caggttgttc
    33241 ccgcaggaag actgtttgcc agttcctcca tttttgtcat tgcatcgccg gaactaaagc
    33301 cagtcgcgtt ttcgccctga atttcatagg ctgccgagcc gttgtagcgc accagtcttt
    33361 ccggtccata cagccagcgg gtggtcgcaa aagcagagaa tggtgtcatg gcgttatcgc
    33421 tgccgcgcac aaaccattta cctaagtctg acggcgcgga acgaaattcg ctgtcgccct
    33481 gaatgtagac ttttttcaca cgaccacggt caataaagtc attcacataa gtaccgcccc
    33541 acgcactgga cagggtgtcg gtgacatcat tcaaactcaa ccctaatgac accgctttat
    33601 tactatcaat atctacctgc aattgcggca tttgtggcaa atcattggcg cgtacagaat
    33661 gtaactccgg actttgattc gctttttcta tcaactgatt acgcatttgc agcagtgttt
    33721 cacgatcggt tccaccgtta gctaacaatt caaacgtaaa accattgctt tggcccagcc
    33781 catcaacggc tggcggcgtc atcgcaaata ccgtggcatc acgaattgtg cccagctctt
    33841 tggttgcccg tagggcgata gcctgggcgg tgttttctgc ccctttacgt tgagaccagt
    33901 ttttcaacga aacaaacgcc atcccggtgt tctgtccgct accgctaaaa ctgaaaccat
    33961 caacggtaaa gatgacatcg gtatttgctt tctcgttaat caggaaccag tcaacaatct
    34021 ggcgattcac ttctgctgta cgggcagcgg tcgccccggc cggcagcgtg tactgcacca
    34081 tgatttcgcc ctggtcttca gtgggtaaga aactgcccgg cagtttccac atcatcaggg
    34141 ccattccacc acccagcacg acataaagcc ccatcgttcg ggctgcacgg cgcaggacat
    34201 aaattacgcc tcgctgatat ttatcttcag tacggcggta aaagcggtta aatgcgccga
    34261 aaaagccttt tttatgtggt ggaacatgct ggaggacgga accgcacagg gcgggagtca
    34321 aggtcaatgc caccaccacg gaaagcagca ttgcggagat gatggtgatg gagaactgac
    34381 gataaattac ccccgtagat cccccaaaga aggccatcgg caggaatacg gctgacaaca
    34441 ccagcgcaat ggcaaccagt gcgccagaaa tctcgcccat tgatttttcc gtggcttcac
    34501 gcgcgggcaa cccttcgtca cgcataatgc gttcgacgtt ctctaccacc acaatggcat
    34561 cgtccaccag caagccgatc gccagcacca ttgcaaacaa cgtcagggta ttaatcgaat
    34621 agccaaacaa cgcaagaacg ccgaatgtgc ccagcaaaac gacggggaca gctaacgccg
    34681 gaatcagtgt ggcacgcagg ttttgcagga ataaatacat cacgcaaacc accaatacga
    34741 tggcttcaaa cagcgtctga attacgtcct ctacggagat tttaataaat tcagtgctgt
    34801 ctttcgggta tgcgatgtcg tatccctgcg gcatgttccg ctggaattcg gcaatcttat
    34861 ccttgaccag cgtcgccgta ttcagcgcat tcgcaccggg cgaaagcatt acagcaaccc
    34921 cggcagacgg gtgaccgtta agtttgccga tagcggtata atcttcactg cccatctcaa
    34981 cgcgagccac atcttttata cgaacaactg cgccgtctga ctggcttttc acgataatat
    35041 ttttgaactg atccaccgtc tgcaagcgag actgcgcacg taccgttgcg gtcagttgct
    35101 gagtatccgg tgaaggcaat gcccctattt tcccggcagt aacctgcacg ttttgcgctt
    35161 caatagcact ttgcacgtct gaaggcatca gcgagtaaga cgccagtttg gccgggtcaa
    35221 gccagatgcg catagcgtat tccgcgccaa agacttgcag actccccaca ccttcaacac
    35281 gcgccagcgg gtcctgaacg ttactgacca gccagtcggc gatatccgaa ctggaggctt
    35341 tgtcagtggt gtcatacacg gcagcaatca gcaaaaagtt gctttgtgat ttctccaccg
    35401 taacacccgt ttgctgcact tcgctgggta ggcgcgactc cgcctgctga attttattct
    35461 gcacctgaac ctgtgcagta tctggatcgg taccttgttc aaaggtcaca ttaatactga
    35521 ccgaaccatc agagctactg gttgagctga aatagagtaa attatcaagc ccagtgagtt
    35581 gttgctcgat aacctgagtc acgctgttct ccagcgtttc ggcagaagca ccagtataag
    35641 tggctgaaat tttaatggtc ggcggtgcaa cgtcaggata ttgcgcgacg ggcaatgtgc
    35701 gaatagccag aatccctgcc aacataatca aaatggcgat aacccaggca aagaccgggc
    35761 ggcgaacaaa aaagcgcgaa aacatcaggc gttgcctcca ttagcctgaa cttcaacagc
    35821 cttgacggtc tgccctgaag tgactttggc agaaccttca acaatcagtc ggtcgccgtt
    35881 gtgcaggccg ttcagcacca gccatttatc accatacgtt tctcccgttt cgagcgttcg
    35941 ctgctctact ttattgtctt tattcaccac cagcgcagtt gcattgcctt tagcatcgcg
    36001 cgtaacgccc tgttgaggcg cgagaatagc gttttccatc acgccttcat cgacacgagc
    36061 gcgaacaaac attcccggta ataactgctg ttgtggattg gggaaaattg cccgtaatgt
    36121 caccgaaccg gtagactcat ccaccgcgac ttcggtgagt tccagacgcc ctttttcgct
    36181 gtaggttgtg ccatcttcca gaataagtga gacgctcatg gtgtcactgt tggtcgccag
    36241 tgactgttta cgcagacgta ataaatcgac actggagcga gtgaggtcga catacattgt
    36301 atccagacca cgaatagtcg ttaacgctgt atcctgcgac gcggtcacca gtgcgccagg
    36361 ggttaccgac gaaataccaa tacgccccga aatcggtgcg gttaccgtgg tccagtcaag
    36421 attaatgcgc gcagtttcca gtgcggcttt tttcgcctct acactggctt tatcttgtgc
    36481 acaggtagac tgagcatcat cagcatcctg ttgtgaaaca ccattctctt tcaccagtcg
    36541 ggcataacgc tgcgctttct ggcaatcggc ttttaccagt gcctgtgctt gttgtaatgc
    36601 tgcccgggct tcattccatg cagcctgata actggccgca tcaatctggt agagaggctg
    36661 tccagccttg accagatcac cttccttaaa taagcgtttc tggataattc ccccaacctg
    36721 cggacgtact tcagcactga gcgcagcact ggtgcgtccg gttaattcac tgactaccga
    36781 aaccggttga gatttgagcg taacaacgcc gacttctgtt tctgacgggg atgaattgtt
    36841 actttgcgta ttatcacaac ccgataagag cagcattgcc actacagatg ttgctatata
    36901 cttcattatt ttctccaggg tgagaaatac cctcctcatt gaaattaatg aggatatgtc
    36961 agggatattc agttataacg aacccgcatc gcgaaatgaa tttcgcaatg aaataagggg
    37021 gattaatatc ccccgctatt ggttaattct gttcttattt atttatcaac atactaacaa
    37081 tatcctgata tatttcctgc agatgttgtg gatcgctttt ttgcggtgtt aaacgacgat
    37141 aaaccgtacc ttccatcatg gtagcaaaca cttcaacgca gcaacgaatg tgctcgtcgc
    37201 taagatgggg atgcattctt ttcatatgtg cacaaccgtt agcgaacatt ctggcgtcag
    37261 cttcttcaag cattgccata acccgcgggt tgcgtgtggc ttctgcagcc acttccagca
    37321 tcagcgcgtc gtcgtcctca tttaaggatt tacgcaaggc aaggacttcc gggaagtgat
    37381 cggtacgcgc gtcaatgtcc atttgggcaa tacgaaagtc gatgatacgg cgaaccatct
    37441 cttcaataat ggcgtctttg ttggcgaagt aacgatatat ctgacccacg ctaagctgag
    37501 cttcgctggc aatttgcgac atactggcgg catgaaatcc gcagcgacga aaacaccgac
    37561 gggcggcgtt aatgatttca tcctgacgct gtttgtggcg tatctccagc ttgctggtca
    37621 tttctgtctc ctggcctatc acttggtgag aatgatcatt ctcatttttg ctaattatac
    37681 gtttctccaa agtaatggaa agtattttta tcttattcat gatgcatatt ctgataatga
    37741 agagattgtg gagattctgt gaagatagtt gacaatatta agttggagtc aattcatcag
    37801 aaagacagcg acttgttgtc gccagttctg ttttcatcat taaacttata ggagctaata
    37861 cgaatagttt caaagtcaga ttctataaga tatttcccca acctccccct tgcaggcgtc
    37921 aggagtagat aacgcaaaat gctctgtacc gcagcttctc tccgctacag aaagcgcaac
    37981 tatagccacc cacagccagg ttgattataa taaccgttta tcagttcgta ctgtttacta
    38041 aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca acaataagga ttaaagctat
    38101 gggttttctt tccggtaagc gcattctggt aaccggtgtt gccagcaaac tgtccatcgc
    38161 ctacggtatc gctcaggcga tgcaccgcga aggagctgaa ctggcattca cctaccagaa
    38221 cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa ttgggttctg acatcgttct
    38281 gcagtgcgat gttgccgaag ataccagcat cgacaccatg ttcgctgaac tggggaaagt
    38341 ttggccgaaa tttgacggtt tcgtacactc tattggtttt gcacctggcg atcagctgga
    38401 tggtgactat gttaacgccg ttactcgtga aggcttcaaa attgcccacg acatcagctc
    38461 ctacagcttc gttgcaatgg caaaagcttg ccgctccatg ctgaatccgg gttctgccct
    38521 gctgaccctt tcctaccttg gcgctgagcg cgctatcccg aattacaacg ttatgggtct
    38581 ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg
    38641 tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact ctggcggctt ccggtatcaa
    38701 agacttccgc aaaatgctgg ctcattgcga agccgttacc ccgattcgcc gtaccgttac
    38761 tattgaagat gtgggtaact ccgcggcatt cctgtgctcc gatctctctg ccggtatctc
    38821 cggtgaagtg gtccacgttg acggcggttt cagcatcgct gcaatgaacg aactcgaact
    38881 gaaataatcg ttctgttggt aaatattggg cggcgttctg ccgcccgtta tctctgttat
    38941 acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct tgcgtcagga
    39001 taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca ccggcaaaag
    39061 ccactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc ctctggtccc
    39121 ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg aagaaacgct
    39181 ggcccccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggagt tgttccctga
    39241 cccgcttacc gtgactcgtt cacagacctt caccgcttat gaacgcttgt cgacggccct
    39301 gacggttgct caggtttgcg gcgtccaacg gttatgtaac tactattctg cgcgacttac
    39361 gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttggcac aaatcacgca
    39421 atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc gccagcatct
    39481 gaatgacgtt ggtcttactg tctgtgactg tgtgatcatt aaccaaatca ttggttttat
    39541 tggctttcag gcgcggacca ttgcgacatt tcaggcttat ctcgggcatc cagtacgctg
    39601 gttacccggg ctggagatac aaaactacgc cgacgcgtca ctgtttgctg atgaatcatt
    39661 acgctggcga agcagctatg aggtggaaaa actccctgaa gagcacacaa aaagttcaac
    39721 tgcagaactt tgccaactgg ccaacacact ctctctccac cctatttcac tttcccttct
    39781 cgaaaagttg ttaaacagca cacgggtcaa tacacagccg gataatcagc ttgcggcgtt
    39841 gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc gcctgtatga attcatcaaa
    39901 tgaatataaa aaaatcagcc cccttctgcg caagggcgaa aatgaaatta accgatgggc
    39961 tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga cccgagcacc
    40021 cgatcgcttt agcgccgccc agttcagccc attactcgaa cacgaaaaat catcaacgca
    40081 gattattaat ctgctagtat ggagcgggct gtgtggctgg ataaatcgct taaaaatcgc
    40141 gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac tgtcagccgc
    40201 tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta gcgcagctta
    40261 aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc acagaaaaag
    40321 gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg ccgcagatga
    40381 aaaaagtcat gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa gaacgtgaat
    40441 ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt aaagttcagg
    40501 gcaaaaatga ccgtctggcc atcgttcctg accatccact cttaaaagac gccattcctt
    40561 gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg tgactgggcg gttgccgaaa
    40621 tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca caatacatca
    40681 cttttggtga cgatcacttt gtaccgtggt gggttaccct tgcgcgtcat aatctggaaa
    40741 aagaagcacc agacggcgtt gctaccgaaa tgctcgatga aggtctggtt cgtgaagatc
    40801 tgactgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg gatgacgccc
    40861 ttttcgccaa ggcgttaccg gatggcaaac ttcagctgat tgtggcgatt gccgatccaa
    40921 ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc gcattcacca
    40981 actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac gatctctgct
    41041 cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc tccactgatg
    41101 gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa gcgaagctgg
    41161 tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggcagcct gaaagcgaag
    41221 caattgccga acaagtccgt ttgctagcac aaatttgcca acgccgcggc gagtggcgtc
    41281 ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc ggtgaaaaag
    41341 gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc gtcgaagaag
    41401 cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc ggttttggca
    41461 tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca gcgttgctga
    41521 aaacgcacgg tctgcatgtc gatgctgaag aagtgctcac gctggacggt ttctgcaaac
    41581 tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt cgtcgcttcc
    41641 agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt ctggaagcat
    41701 acgccacttg gacttcgccg atccgtaaat atggcgacat gatcaaccac cgtctgctga
    41761 aagcggttat caaaggcgaa actgcgacgc gtccacagga tgaaatcact gtccaaatgg
    41821 ccgagcgtcg ccgtctcaac cggatggcag aacgtgatgt tggcgactgg ttatacgcac
    41881 gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc ggcggaaatt gtcgatatca
    41941 gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc tatcgccttt attccagcac
    42001 ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc actgtacaaa
    42061 ttaaaggtga aacggcttac aaagtgactg acgttattga cgtcaccatt gccgaagtcc
    42121 gcatggaaac ccgcagcatt attgcgcgcc cagtcgcgta atctcctttc acggcccatt
    42181 cctcatggat gggccgttta tttccccgct caaccttcgt catatcccgg cagattttta
    42241 actgactttc gtttgaaaac tggcgttttt ccaaaactgg attacaaata aatatataaa
    42301 aaaacaatgt gtttagccgc cgttaatttc atgtaagtca tgttgttcct tttatttaac
    42361 tgcggactcc gctgttaacc ggaggatatg catcatgaaa accgttaggg agtccacaac
    42421 gttgtacaac tttctcggat cgcacaatcc atactggcgg ttgacggaaa gcagcgatgt
    42481 tttgcgcttt tctaccaccg aaatcacaga acccgatcgt attttgcaac tatctgccga
    42541 acaggctgct cgcatcaggg aaatgacggt catcacctcc agcctgatga tgagtctgac
    42601 cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga aaaatcaata aacgagaatg
    42661 gggaggcaac gcgtctgcat ggcatgacac accggcggtt gctcgtgatt tatcacatgg
    42721 gctttccttt gctgagcagg tagtttctga agcacattcc gcaatagtga ttctcgacag
    42781 ccgggggaat atccagcgct ttaatcggtt atgtgaagat tacacaggct tgaaagaaca
    42841 cgacgtcatt gggcaaagcg tgtttaaact gtttatgagt cgtcgtgaag ctgcggcatc
    42901 caggcgcaat aaccgtgtat tttttcgaag cggcaatgca tatgaagtcg aactgtggat
    42961 accaacacgt aaaggccagc ggctgtttct gtttcgcaat aaatttgtcc acagcggcag
    43021 tggcaaaaac gagatttttt taatctgttc cggcaccgac attaccgaag agcgccgcgc
    43081 tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc cgaatcgtaa
    43141 cgcaatgcag gagttaatcg atcacgctat taatcaggca gataacaata aagttggggt
    43201 tgtgtatctt gatttggata attttaaaaa ggtcaacgac gcctatgggc atttgtttgg
    43261 tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac atgaccaggt
    43321 attggcgcgt ccaggtggcg atgagtttct ggtactggca tccaatacct cacaaagcgc
    43381 gctggaagca atggcatcac gaattttgac ccgcttacgg ctcccctttc gcattggttt
    43441 aattgaagtt tataccagct gttcagtagg tatttcactc tctcccgaac atggttcgga
    43501 cagcgcggct attattcgtc acgccgacac agcaatgtac acagcgaagg aaggcggacg
    43561 aggacaattt tgcgttttta ccccagaaat gaatcagagg gtatttgaat atctctggct
    43621 ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact atcaaccgaa
    43681 aatcacctgg cgtggtgaag tgcgcagtct ggaagcacta gtacgttggc agtcacctga
    43741 acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagaatcag ggctaattgt
    43801 gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtagcgaagt ggcgggataa
    43861 aggcattaac ctgcgagtag cggtaaatat ttctgcacgt cagctcgccg atcaaaccat
    43921 tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc ctatagatgt
    43981 tgaactgacg gagagctgcc tgattgagaa tgatgaactg gcactgtctg ttattcaaca
    44041 atttagccga ttaggtgcgc aagttcatct ggacgatttt ggtaccggct actcttcact
    44101 ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt ttgttcggga
    44161 tattcacaaa cagcctgtct cgcaatcact ggtccgggcg atcgtcgctg tggcccaggc
    44221 attgaatctt caggtgatcg ccgaaggtgt agaaagtgca aaggaagatg cttttttaac
    44281 caagaacggg atcaatgagc gacaaggatt tttgtttgcc aaaccgatgc ccgccgtcgc
    44341 cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat atatatactt
    44401 agcgctatat tccgtcatta tatatcgtat tcccggatga taattagccg ttgctgttaa
    44461 acccttctct tttagcggca atttttgcat tattcatttt cccccttttg ctataaggat
    44521 gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata ttgtgttaga
    44581 tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac ttaaagcaga
    44641 actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt gggagattta
    44701 acaatcgtgc cttcaggttt tcctatttga ggatgccggc gcgtcagtat ttttttattt
    44761 agtattataa cgttataaga attacagcga tgaattcccg acaacaaact attctacaga
    44821 tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact ggagtttctg
    44881 aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc cgacgtgcac
    44941 atggttttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg agcaactata
    45001 cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc gaaaccatct
    45061 ttatcgaaaa tggtagcagc aatgccctgc tcgcccggac tctgggcgag cagaagaaaa
    45121 atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac gcgccttgtg
    45181 aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt ggccctttga
    45241 cacgccagtg catccaacag gtgcatttca gcaaagcatt tattggtatt gatggctggc
    45301 aacctgaaac tgggtttacc ggtcgcgata tgatgcgtac cgatgtggtc aatgccgtgc
    45361 tggaaaaaga gtgcgaggcg atagtcctga ccgacagctc gaaatttggt gctgtacatt
    45421 catactccat cggccccgtt gagcgattca atcgcgtgat taccgattcg aaaatacgcg
    45481 ccagcgatct gatgcatctt gaacaaagta aactcaccgt tcacgtcgtt gacatttaac
    45541 catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta agatgattcc
    45601 tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa aattattatc
    45661 agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc gagcggattt
    45721 cacggaataa tttcacctgg cttattctta gctattatag ttatagagag cttacttccg
    45781 tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat gaccgcggct
    45841 gttctggcaa ttaccctggc aatgtctctg agtgcctgtt ctaactggtc taaacgggac
    45901 cgcaacaccg caatcggcgc aggtgcaggg gcattaggcg gtgcagtact gaccgatggc
    45961 agtacgttgg gtacattagg tggtgcagcc gtcggtggtg ttattggtca ccaggtcggt
    46021 aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt gattctgttc
    46081 agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg atatatccgt
    46141 ggctttttat gttagccgcc tgcaagtttt accttcatcc ctttcgcttc aagcattgat
    46201 tttaataaat cacgcttatc gccctggatt tcaataattc catcttttac tgctccgccg
    46261 cagccgcatt ttttcttcag ttcagctgcc agtttcgtca gttcggcatc atcgagatcg
    46321 acaccggtaa tcaggcaaac gcccttacct ttgcgtccac tggtctgacg ctgaatacgc
    46381 accacaccgt cgcctttagg gcgtacaggg gccgcttttg gctcatcaat acgtccggtt
    46441 tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc tgtaaagagg
    46501 cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg cgaccaatca
    46561 ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg cgctggtcgc
    46621 cagcatcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc tgaccgaata
    46681 cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg ccacattttt
    46741 gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcacg ccaagatcgg
    46801 ccaggtcgca ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc ggtgcatctt
    46861 tgccaaacgg aaccagtgcc tcacgcgctg cggccatcat acgcgcccca ccggaggcat
    46921 gaacattcac catccacacg cctaagtcag ccgcagcagc gacagcgtgc gctgcagtgt
    46981 tgggaatatc gtggaatttc aggtcaagaa agatatcaaa atcacgctgt tgaagttcgc
    47041 gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga cgacaatcgc
    47101 gtgggtcgat cttgtcgaca aaggccagcg cggcatcgcg attatgataa tcaagggcaa
    47161 caactacagg agaattcgta acagcgcggg aagaagatga agcagttaac gtcatgacca
    47221 gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg agcattctac
    47281 ccgtggaaat aaaaaattaa cagttgcgat ttctcctctg gcaggctttt ccgcgccgtc
    47341 ggtaccggaa cacaatagaa gatcaataat tagtatgttg taactaaagt cggatttttt
    47401 taaaaattac aggccatcaa gaccgcgaat tggtttaatg gttgaccagg cccgacaaga
    47461 cggacaatgc cagtagaggg tgtatgcggt aaaaccacat ttctggcagc gataacgagg
    47521 cttactacgt accttctcgc caaccatgtc acgcagcacc atcagactct ctttggcacg
    47581 cccttcttct gcttcattta agtggtaatc cattaattta tggaacacac gcatggtcgg
    47641 atgacgctga agctggcgcg taatatagac ctgtgcagcc tcactaccgt cgcgcgcttc
    47701 gatgatatcc gcaagcatca attcagcatc ggcaccggtg ttctcttcca ccgcgcgctg
    47761 caggaattct gcccattcgg cagttttacc caacagctga tagcatgttt gcagcatttc
    47821 cagcgtttcg ctgaccagtt ctctgtcctg ggatatgaca cgttgcaaac tttcgacggc
    47881 tttggcgtat tctgctttcg ccataaacac gcgtcccatc atgatggata cgcgggcgct
    47941 gtttttatct gccgccgcac ctttttttag caaagtcatg gcacgatcga gatcgtcgct
    48001 ggccatatgc tgcagagcta actcacagta gaaatgggca atttcgacgc gctgtttatc
    48061 tttacccagc ttcaccaggc gttcggcaac atcaattgct ttctgccact cgctggtagc
    48121 ctggtaaatt tgtagcagtt gttgcagcgc gccgatgcgg aagtcagttt catctgtcag
    48181 ctgattgaac atgtcttccg cgcggtcgta taacccggca gccatgtaat cacgccccag
    48241 ttgttgaatc gccaaaagcc gctgttcata ggtcagcgag gcgctttcca ttagggtctg
    48301 atggatgcga atagcgcgat caacttcgcc acgcgaacgg aacaggtttc cgagcgtaag
    48361 gtgggcttca acggtacctg tatcctcttt aagcatatcg agaaacaggt ctaccgcttt
    48421 atcctgttga ttactaagca ggaagttaac ccccgctacg taatcacgcg acaagcggtt
    48481 ggcttcatct tgcttgtttt gttgcgcact tctgcggccc atataccagc cataggcagc
    48541 ggctacaggc aaaagcagaa acaacaactc cagcatagaa agttattcct tcgccgcaga
    48601 cgagtgcggc gctacagcca cgtcagtcgc gggtgaaagc tggttttcca gtcgctttat
    48661 tttacgttca gcgcgcgcca gggaaacacg aactcgcagc cagaacaggc cgcaaatcaa
    48721 ccaaccgata gcaaaccccg cagcaaacaa taccgccagc aatgtggaaa tacggtactc
    48781 cccttgcgct aacagataat taaacgtcac ctgttgatcg ttctgcgcac ccaacgtgac
    48841 cgaaatcacg aagatcgcta acaccagtaa gaaaatgagt aaatatttca cattacttcc
    48901 cgttatgtgg ttagagcgaa taaattatgg tcaacttacc gcaatagccc tttaaagtac
    48961 cattttcgcg ccgtctgcga aatggcatcc acgcgcattg ttgatgattt aagggctaaa
    49021 ttcggaaaat caaccagcgt taactttctt gttctcgttg cgctatttcg cgattttctt
    49081 ccgcaggtgg tgttaatggc ccacaaattc gttgcgcaag ccacgttgcc accgccacca
    49141 gcgcccacga aatcaacgta gctacaacca gatcgcgtgg ccaatgcatc ccgagcagca
    49201 ggcggcttcc catgactccc gttgcccaga ccaacaagat agcaatggtt aacgttcgcc
    49261 gacgcggcca cagcaaacca acggccagca gtgcccaact ggcagcaaac atcgtgtgac
    49321 cggaaggaaa ggcaaacccc gtctctttct gccagtgtga acgcaaatat tgtgggatat
    49381 ttttctcttc agccaactgt tctttcacca gatttccgcg ttctgctcgc tttaaagtgt
    49441 agaactcatc aaccggaata tgatgtgttt tttccagcca gataacaaaa ggtcgtggtt
    49501 cctggacttt gtctttgatc caggatttaa cgccttgtcc cataaggatt gctgccgcca
    49561 gaatggcaaa taacataatg gcagccttaa tgcgaaaacg cagacaccag agaaaccagc
    49621 cgaataaaat caaatgtgta atgacgcccc agggctgggt gacagtttca gtaacccaaa
    49681 aagccgcttt tagtaaccaa ctttgttctc caggttgcca acgccagcca gaaatccata
    49741 cggctactgg catgacaagc aatagtgcag ctcccactgc ggtacgtctg gcaatcgaac
    49801 gcatggcctc tcctttttga taagtcccac aatcataact gaaaatgcca gttccaggaa
    49861 aaattgacag atttgtgcca ttccgtgaac gatcgacgcg tcgtgattag gtgaacccct
    49921 tctcgttatg gcaaaataag ccaatacaga accagcatta tctggagaat ttcatgcagc
    49981 ttaaacgtgt ggcagaagcc aaactgccaa ccccatgggg cgatttcctg atggtgggat
    50041 ttgaagaact ggcaaccgga cacgatcatg tcgcgctagt ctatggcgat atttccgggc
    50101 ataccccggt acttgcgcgc gtccattccg aatgtctgac cggtgacgcc ctgttcagct
    50161 tgcgctgcga ttgtggcttc cagctcgaag cggcattgac gcaaattgcc gaggaaggcc
    50221 gtggtatttt gctgtatcac cgtcaggaag gtcgtaacat tggtctgctg aataaaatcc
    50281 gcgcttacgc actgcaggat caaggttacg atactgtaga ggctaaccac cagttaggct
    50341 tcgccgctga tgagcgcgac ttcactcttt gcgctgatat gttcaaactc cttggcgtca
    50401 atgaagtccg cttgttaacc aataacccga aaaaagtcga aattctgacc gaagcaggga
    50461 ttaatattat tgaacgcgta ccgttgattg taggtcgcaa ccccaataac gaacattatc
    50521 tcgataccaa agccgagaaa atgggtcatt tgctgaacaa ataaccctct tgcattgtgt
    50581 aattaatttg cttgctggaa gcaaaataac cggcaagcaa atactcgtta cttcaacata
    50641 ttacgaatga cataatgcaa aatgccgtcg ttctggtagt aggtcaactc cgtcgcggtg
    50701 tcgatacgac aatggcaggg tacgacttcc tggctaccat ccgcgcgcgt aagcgtcacc
    50761 ggaaccgtcg cgccgggttg tagattttgc agatcaccaa tatcaatctt ctcttccccg
    50821 gtaagctcta acgttttacg cgtcacacct tgtggaaatt ccagcggcag gatgcccatg
    50881 cctattaaat tcgaacggtg aattcgttca aacgattcgg caatcacaac acgaatacca
    50941 agcagacgcg gaccttttgc cgcccagtca cggctggagc ctgatccata ctctttcccg
    51001 gcaatcaccg ccagcggcgt ttgctcctgc ttatagcgca ttgcggcatc ataaatagag
    51061 acgacatcgc tgtcaggtaa atggcgcgtc atcccccctt caacgccagg caccatttca
    51121 ttacggatgc gaatattggc gaaggtgccg cgcatcatca cttcatggtt accacgccgc
    51181 gaaccatagg agttaaagtc tttccgctca acaccccgac cttgtagata tcgacccgcc
    51241 gggctgtcgg gcttaatact gcccgccgga gagatatggt cagtggtgac tgaatccccc
    51301 agcattgcga ggatccgcgc accgtgaata tcttccacag gtgctggtgt tgcctgcatt
    51361 tcatcaaaga aaggcgataa gcgaatatag gttgaatcct cctgccaacc gtaggtatcg
    51421 gatcgtgtga cgttaatttc cttccactcg gctgtgcctt caaaaacttc tgcgtactct
    51481 ttgcggaaca tttctgtgga gacttggtct accgcacggg caatttcttg tgccgatggc
    51541 cagatatctt tcagaaatac cggctcgcct ttgcgatcat ggccgatagg ctcagaagcc
    51601 agattgatat tcatatttcc cgccagcgca taggcaacca ccagcggcgg cgaggccagc
    51661 cagttggttt taaccagcgg atggatacgg ccttcaaagt tacggttgcc ggacagcact
    51721 gcaccgacgg ttaaatcgcc ttttttgatt gccgtttcga taggatcggg caacggtccc
    51781 gagttaccaa tacaggtggt acaaccgtat cccacaaggt taaatcccag ttcatcgaga
    51841 tacggtgtca gtttcgcttt tgccagataa tcagaaacga ctttcgaacc cggtgccagc
    51901 gacgctttga cccatggttg ccgcttgagg cccagagtta cggctttttt cgccagcaag
    51961 cctgcggcca tcagcacact tgggttagag gtgttggtgc acgacgttat cgcagcaatg
    52021 accacagcgc catcaggtaa ctgatactga tgtccgttca taacataatc gaccggcagg
    52081 cgatctttat gcgtggcatt cacttccagt tcgctactgg cagcaaatgc ttttggtaca
    52141 tcgggcagtg caacgcgatc ctgcgggcgt tttggccctg ccaggctcgc ttcaacgtca
    52201 ttcatatcca gttctaacac actggtaaaa attggttcat cgcccggatt acgccacatg
    52261 ccctgcgctt tggcatattt ttcgaccaac tcgacctgat cttcactgcg cccgcttaaa
    52321 cgcatgtaat cgagggtgac agcatcgatt gggaagaagc cacaggtggc accatattct
    52381 ggcgacatat tggcaatggt ggcgcgatcc gccagcggta gtgaatccag cccatcacca
    52441 taaaattcga cgaatttccc caccacgcca tgtttgcgca acatttgggt aacagtgaga
    52501 accaggtctg tggcggtaat accttcacgt aattttccgg taagtttaaa gcccactacg
    52561 tccgggataa gcatggaaac cggctggcct aacattgcgg cttctgcttc gatgccacca
    52621 acgccccacc ccagcacgcc aaggccgttg atcatggtgg tgtgcgagtc agtaccaacg
    52681 agtgtatccg gataagcaat ccattcaccg tcctgcaatt cgctccacac tgctttgccg
    52741 agatattcga ggttaacctg atggcaaatg cctgtgcctg gcggcacgac gctaaaccga
    52801 ctgaacgcct gctttcccca tttcaggaac acataacgtt cgtggttgcg ctccatttcc
    52861 aggcgtacgt tttcttcaaa tgcctcatca tcaccaaaac gatcgacggt caccgagtgg
    52921 tcaatgacca ggtcgaccgg tgagagcgga ttaacctttg cagtatcgcc gccgaggcgt
    52981 ttaaccgctt cgcgcattgc cgccagatca acaacggcag gtacgccggt aaagtcctgc
    53041 atcagcactc ttgccgggcg gtaggcaatt tcacggtcag catgggcatt tttcagccat
    53101 cccgccagcg cgtggatatc ctcttcggta accgatttac catcctgcca acgcagcagg
    53161 ttttcgagca aaactttgaa tgacttgggt agacgggtga tatcgcccag tgatttggca
    53221 gcaagcggca agctgtagta gtggtaagtt ttatctttgg cctgcaacgt gtccttactg
    53281 gcttctcgta gggttgacga catagctcct ccttaatgac agggttgcga agccctgatt
    53341 ctcttcaggg tgaatattaa agataacaca aacatcgcgt aacgatttga taacaaccca
    53401 aattgataaa agaggagaaa ccttgaggca aagcaaacag cgggatcagg gatcagtgag
    53461 taatgatcca gaggagttcg gcccagaaga gcatcgacag agaaaatgcg cccatccagg
    53521 accaatattt catatttgtg ttgatcatag gctacaccct taacggttat tactttgtgt
    53581 tgagataact caacctgtta ttgctgcggt gtattaaaag ccctgtaatt cattaaaaat
    53641 gggtaataag aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat
    53701 aaaggcttgt tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg
    53761 ttttgcttct ggttcattgt cactatgctg acataatggt gacgactcca gttgctcctg
    53821 accgaccatg gttatcccca caatttccaa ataagcagtg cgacaaccac ccagaaaagt
    53881 gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta attcaggatt gcggcggggt
    53941 tcctgatttc cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg
    54001 ttgaacgatt ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagtcaaa
    54061 aatccagcga atttacgaat cgtctccagt g
//
LOCUS       DACADA010000011        53215 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.011, whole genome shotgun sequence.
ACCESSION   DACADA010000011 DACADA010000000
VERSION     DACADA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.011"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..1982)
                     /locus_tag="ISB17_000665"
     CDS             complement(<1..1982)
                     /locus_tag="ISB17_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP1978641.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEV"
     gene            complement(1979..3520)
                     /locus_tag="ISB17_000666"
     CDS             complement(1979..3520)
                     /locus_tag="ISB17_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP1978642.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            complement(3530..4306)
                     /locus_tag="ISB17_000667"
     CDS             complement(3530..4306)
                     /locus_tag="ISB17_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAP1978643.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4316..5158)
                     /locus_tag="ISB17_000668"
     CDS             complement(4316..5158)
                     /locus_tag="ISB17_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP1978644.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5168..5959)
                     /locus_tag="ISB17_000669"
     CDS             complement(5168..5959)
                     /locus_tag="ISB17_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAP1978645.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6172..6819
                     /locus_tag="ISB17_000670"
     CDS             6172..6819
                     /locus_tag="ISB17_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1978646.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(6803..7441)
                     /gene="ytfB"
                     /locus_tag="ISB17_000671"
     CDS             complement(6803..7441)
                     /gene="ytfB"
                     /locus_tag="ISB17_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAP1978647.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     ESQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7660..8280
                     /locus_tag="ISB17_000672"
     CDS             7660..8280
                     /locus_tag="ISB17_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1978648.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8589..10001
                     /gene="cycA"
                     /locus_tag="ISB17_000673"
                     /gene_synonym="dagA"
     CDS             8589..10001
                     /gene="cycA"
                     /locus_tag="ISB17_000673"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAP1978649.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10046..10708)
                     /gene="ytfE"
                     /locus_tag="ISB17_000674"
     CDS             complement(10046..10708)
                     /gene="ytfE"
                     /locus_tag="ISB17_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAP1978650.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(10816..11781)
                     /locus_tag="ISB17_000675"
     CDS             complement(10816..11781)
                     /locus_tag="ISB17_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1978651.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(11889..12749)
                     /gene="qorB"
                     /locus_tag="ISB17_000676"
     CDS             complement(11889..12749)
                     /gene="qorB"
                     /locus_tag="ISB17_000676"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP1978652.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETDKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12838..13218
                     /locus_tag="ISB17_000677"
     CDS             12838..13218
                     /locus_tag="ISB17_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP1978653.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13347..15290)
                     /gene="cpdB"
                     /locus_tag="ISB17_000678"
     CDS             complement(13347..15290)
                     /gene="cpdB"
                     /locus_tag="ISB17_000678"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAP1978654.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15480..16220
                     /gene="cysQ"
                     /locus_tag="ISB17_000679"
     CDS             15480..16220
                     /gene="cysQ"
                     /locus_tag="ISB17_000679"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAP1978655.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16210..16767)
                     /locus_tag="ISB17_000680"
     CDS             complement(16210..16767)
                     /locus_tag="ISB17_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP1978656.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17092..17298
                     /locus_tag="ISB17_000681"
     CDS             17092..17298
                     /locus_tag="ISB17_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAP1978657.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17360..18703)
                     /locus_tag="ISB17_000682"
     CDS             complement(17360..18703)
                     /locus_tag="ISB17_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP1978658.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19026..19664)
                     /gene="msrA"
                     /locus_tag="ISB17_000683"
     CDS             complement(19026..19664)
                     /gene="msrA"
                     /locus_tag="ISB17_000683"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP1978659.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19870..21603
                     /gene="tamA"
                     /locus_tag="ISB17_000684"
     CDS             19870..21603
                     /gene="tamA"
                     /locus_tag="ISB17_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAP1978660.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21600..25379
                     /gene="tamB"
                     /locus_tag="ISB17_000685"
     CDS             21600..25379
                     /gene="tamB"
                     /locus_tag="ISB17_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAP1978661.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25382..25723
                     /locus_tag="ISB17_000686"
     CDS             25382..25723
                     /locus_tag="ISB17_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP1978662.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25935..26186
                     /locus_tag="ISB17_000687"
     CDS             25935..26186
                     /locus_tag="ISB17_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpS family
                     antitoxin"
                     /protein_id="HAP1978663.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            26180..26530
                     /gene="chpB"
                     /locus_tag="ISB17_000688"
     CDS             26180..26530
                     /gene="chpB"
                     /locus_tag="ISB17_000688"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HAP1978664.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(26610..27140)
                     /gene="ppa"
                     /locus_tag="ISB17_000689"
     CDS             complement(26610..27140)
                     /gene="ppa"
                     /locus_tag="ISB17_000689"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAP1978665.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            27450..28406
                     /gene="ytfQ"
                     /locus_tag="ISB17_000690"
     CDS             27450..28406
                     /gene="ytfQ"
                     /locus_tag="ISB17_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAP1978666.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            28546..30048
                     /locus_tag="ISB17_000691"
     CDS             28546..30048
                     /locus_tag="ISB17_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1978667.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            30062..31084
                     /locus_tag="ISB17_000692"
     CDS             30062..31084
                     /locus_tag="ISB17_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1978668.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            31071..32066
                     /gene="yjfF"
                     /locus_tag="ISB17_000693"
     CDS             31071..32066
                     /gene="yjfF"
                     /locus_tag="ISB17_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAP1978669.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(32099..33097)
                     /gene="fbp"
                     /locus_tag="ISB17_000694"
     CDS             complement(32099..33097)
                     /gene="fbp"
                     /locus_tag="ISB17_000694"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAP1978670.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            33273..34646
                     /gene="mpl"
                     /locus_tag="ISB17_000695"
     CDS             33273..34646
                     /gene="mpl"
                     /locus_tag="ISB17_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAP1978671.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(34797..35348)
                     /locus_tag="ISB17_000696"
     CDS             complement(34797..35348)
                     /locus_tag="ISB17_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAP1978672.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            35442..36794
                     /gene="pmbA"
                     /locus_tag="ISB17_000697"
     CDS             35442..36794
                     /gene="pmbA"
                     /locus_tag="ISB17_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAP1978673.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            36977..37363
                     /gene="cybC"
                     /locus_tag="ISB17_000698"
     CDS             36977..37363
                     /gene="cybC"
                     /locus_tag="ISB17_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAP1978674.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(37408..37872)
                     /gene="nrdG"
                     /locus_tag="ISB17_000699"
     CDS             complement(37408..37872)
                     /gene="nrdG"
                     /locus_tag="ISB17_000699"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAP1978675.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(38031..40169)
                     /gene="nrdD"
                     /locus_tag="ISB17_000700"
     CDS             complement(38031..40169)
                     /gene="nrdD"
                     /locus_tag="ISB17_000700"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP1978676.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(40563..42218)
                     /gene="treC"
                     /locus_tag="ISB17_000701"
     CDS             complement(40563..42218)
                     /gene="treC"
                     /locus_tag="ISB17_000701"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP1978677.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(42268..43689)
                     /gene="treB"
                     /locus_tag="ISB17_000702"
     CDS             complement(42268..43689)
                     /gene="treB"
                     /locus_tag="ISB17_000702"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAP1978678.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGNFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(43808..44755)
                     /gene="treR"
                     /locus_tag="ISB17_000703"
     CDS             complement(43808..44755)
                     /gene="treR"
                     /locus_tag="ISB17_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAP1978679.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            44940..44993
                     /gene="mgtL"
                     /locus_tag="ISB17_000704"
     CDS             44940..44993
                     /gene="mgtL"
                     /locus_tag="ISB17_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAP1978680.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            45134..47830
                     /gene="mgtA"
                     /locus_tag="ISB17_000705"
     CDS             45134..47830
                     /gene="mgtA"
                     /locus_tag="ISB17_000705"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP1978681.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(48036..48422)
                     /gene="ridA"
                     /locus_tag="ISB17_000706"
     CDS             complement(48036..48422)
                     /gene="ridA"
                     /locus_tag="ISB17_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAP1978682.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(48495..48956)
                     /gene="pyrI"
                     /locus_tag="ISB17_000707"
     CDS             complement(48495..48956)
                     /gene="pyrI"
                     /locus_tag="ISB17_000707"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAP1978683.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(48969..49904)
                     /gene="pyrB"
                     /locus_tag="ISB17_000708"
     CDS             complement(48969..49904)
                     /gene="pyrB"
                     /locus_tag="ISB17_000708"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAP1978684.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(49908..50042)
                     /gene="pyrL"
                     /locus_tag="ISB17_000709"
     CDS             complement(49908..50042)
                     /gene="pyrL"
                     /locus_tag="ISB17_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAP1978685.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(50323..50718)
                     /locus_tag="ISB17_000710"
     CDS             complement(50323..50718)
                     /locus_tag="ISB17_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP1978686.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(50849..51562)
                     /gene="bdcA"
                     /locus_tag="ISB17_000711"
     CDS             complement(50849..51562)
                     /gene="bdcA"
                     /locus_tag="ISB17_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1978687.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            51633..52226
                     /locus_tag="ISB17_000712"
     CDS             51633..52226
                     /locus_tag="ISB17_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1978688.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            52371..52823
                     /locus_tag="ISB17_000713"
     CDS             52371..52823
                     /locus_tag="ISB17_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1978689.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            52946..>53215
                     /locus_tag="ISB17_000714"
     CDS             52946..>53215
                     /locus_tag="ISB17_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978690.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKCSNEAGRNMMAHIESSSY"
BASE COUNT        12647 a        13458 c        14124 g        12986 t
ORIGIN      
        1 acttcggtaa taaaagaacc ttgcgaatat tgaattgtta atttgtgatt tttgtcttca
       61 gcgagttcag agcgaaaacg ccattcttct tttgcgccgc gacgagtttg tggtaggcat
      121 ttgccttgtg gatcaataac catacgcgcc ccacggctac caccaccagc atcaatataa
      181 tgcgttaatt gtgttaatac cgcggcagaa gtcagcgtca tatgccgcca cataaacagc
      241 tcggcaactt cacccacatg tttgattgcc aatccatgtc gttggacaaa ttcgttcaat
      301 agcagggcat cacgagtggc gcgtcggact ttatcggcat ggcaaataaa tcccgcatgg
      361 tcagacattc gtgcctgaat tttttctctc acaaccgaca acggcatccc ggtcccgtta
      421 tcgtgcgcct gagtaatgat ctctcttata gaagccagtg ccggagctgc cagctgtgct
      481 atatctccat caatattccg cttttgcgtg caaccaataa aacgtgccag acgaacagca
      541 aaaacctgcc cggcattcaa tgccgcgccg ccaggtcggg tgacgccgtg tgtgccagca
      601 acttccccca ccgcaaaaca accgggcaga gatgtttgtc cccagatatc gacttcaatg
      661 ccgccattca tgtgctgatt attcatggca aactgcaatg gctgcgtggt gagatcgtaa
      721 ccgtgcatct tatacagcga gatagacagc ggattcattc gttgcaggcg ttcgatgggc
      781 gatggtgcca gagcgtcgtt attttccaga tacgtgcgaa cgtcgtcgtc cagtcgatct
      841 aatgagaagg gcaggtcacc cggcaccgct tcaggattgc gattgaaatc cataaatacc
      901 tgacgccctg attgctgctc ttgcgctact gccatatcta acaagctgga gccaaaatcc
      961 attacccgcg tggcgtggaa cggccactga tagcctttac ggaagatgtt tgaagctagc
     1021 tcctgggtgg tgcgatagta atccgcgagg aagttatact cgttaccctc agcatctacg
     1081 gaatagatat acgggatcac ctgcacatag gtgccggaca aattccacgg aaacgtgctg
     1141 cgcggtgtgc cgataccaaa ctggctttca gtcagattgg ttagcgtcag accttcctcc
     1201 agcgccagac ccagcgagcc gaaacatttg tgtggataaa cactgtcgcg ataaagctcg
     1261 ccaggccctc ctgttgccag taccacattg ggcgcagtca caattgccag cccccatggg
     1321 ttatgggcgc gatgaccggt cgcgaggatt gcccccgcca cacggtcttc accgttttcg
     1381 tcacgctgat gcagcagttt aatcactgtt gcactggtca acattgggat agcaagacgc
     1441 tgtacttctt ccagcagcac tttcaccatt aaccttgagg tacgcggccc acacgaggtt
     1501 gcacgcccgg cttcgtcatg gtcggtttga taacgaagaa tcgcgccata gcgatcttcc
     1561 ggtaattcca gaccaagata ttgcaacccg ccaagagtgt gcagagaacc taccgcttca
     1621 acataagcgg tgtcgtgatc catcgcccca ccgctcgcca gtgcctctgc cagtttggtg
     1681 aagttgtcgc cgttgcccgc agtagcggcg gtaaacagtg tttgtttatc agagccagaa
     1741 catgccgacg tccccatata taacccggcg gtggcgatta acacattttg ctggcggcgt
     1801 ttaagttcca cagctgcacg tagcccggct gcaccactgc cgactaccag ggcatctgtt
     1861 ttccagagtg ggtaatcaac gccgtcaacc gtgacaaccc atgcgggttc tgcctgtgca
     1921 aaaccctttg ggaaagtaac gtgcgtcagt gcatcaagga tggtgtcatc aagctgactc
     1981 atgcatcctc cattggcata aacaggctac tttccatcag acgtgggtgc ctgataatcg
     2041 gacggaaggc catatacggc agaatgtcgc gctcaatctc cacacccggc gcgatttcaa
     2101 ttaattcaac gccttctgcc gttaactgga atactgcgcg ttcagtgata tacaacactt
     2161 cctgaccact ttgctgtgcg taggcggcgc taaaagtaat tttttccacc tgggtaacca
     2221 gtttagggat ttgacctgac tgggcgatat gcaagccatc tggcgttaca tcaatcttgc
     2281 tgcctttggc gtcgaacgta ccgcaaaaca ccactttacg ggcgttttgt gcaatttcga
     2341 gaaatccgcc ggggccaatc agattgccat tgagatggga tacgttgacg ttaccgtatt
     2401 gatccatctc tcccatgccg aggaaggtga tatcgatgcc gccgccactg tagaattcaa
     2461 attgatcgag tgatggaata atggcatcgg cgttacgggc gcagccgaac agagcatcat
     2521 caagcatcat gccgttatgc acaccctgtt ctacactcag ccacaaactt tgataaggca
     2581 cgccttcgcg cagcgcaacc ccaggaatac cgccgggaat accaaagcca aagttggttg
     2641 aagcgcctgc caccagttcc cgcgctgccc gacgggcaat caggcgacgc accggatgac
     2701 taaccagttc aatagcgtcg ttagaactta gacggcgatg ttgaccgctg atggtcaggt
     2761 cgtaaccacc aagataggtt tgcggttgtt cgcggtgctc aacgatgcca tcaaccagaa
     2821 ttcctggtag cttgacccga cgtggttcaa tggtacctgc ttccagcaca tcgcgtacct
     2881 gtacgaacac tttgccgccg ctgttgtgtg ctgccagcgc catcgaatag ctatccatat
     2941 caatcgcttc ttcttcgagg ctaacgttgc ctcgtggatc ggcataagtg ccacgcaaaa
     3001 tcgcgtaatc cactttgaaa gggcgataac gaagtttggt ctcaccgtcg atttcaatca
     3061 gttctaccag gtcgtcggta gtgcattcat tcgacttacc gccaccattg cgtggatcaa
     3121 caaacgatcc cagcccaacg tgggtaaaaa gccccggacg tccggcaccg atctcccgca
     3181 gcagtgcctg aattacgcca ccagggaaac agtaggcttc aatagcattg tttttcacca
     3241 gttcctgcat cttgggcgac caggtaaaat gtccggcaat aatgcgtttg agcatttcgg
     3301 cgtgagcaaa acggttagtg cctttgctgt cgcgatcgcc aatacccagc gagtggatta
     3361 atgtcagatc gcgcgggtgt ccggtttgca gaaagcgcgc ttcaatggcg gccagaatat
     3421 ggtcagcttc caccatgccg ccgccgttgc cgctaatggc aatggttgcg ccgtcctgaa
     3481 tttgcgccgc cagtgtctcg gcggttgata acttacgcat acaatttcct tattgattgt
     3541 ggaagctcac gccggctttt tcggcaaatg cgtgcgtcgc ctgttggcaa tcctgagtgg
     3601 cggacattaa tccagccagc aactggtgcg agtaatcggc gtgtttattt tccagcgcca
     3661 gaatcagttg cttaatatgt gccagtgcca ccggaccaca tttttccagc tgcgctaact
     3721 ggttcgccat ccagctttcc acctgttctt tttctaccac ttcatcaatt aaattcgctg
     3781 tctgagcttc ctgcgccgtc aggcgtttac ccagcatcag catctggcga ccgaccacag
     3841 gaccaacctg attaagcact cgctcaatgc ccatccagcc tggcaccatg ccgagcatca
     3901 cttccgggtt agagaatttc gcgccggggc gagcgatacg aatatcgcag cacaaggcaa
     3961 gttcaatacc accgccgatg gtatgaccgt tgagattcgc aacggttaat tggggtaagt
     4021 ttcgcaggcg attaaaaacg tcattaccgc gtttaatcca ttttctgccc atatctaatg
     4081 gcgaataggc cgaccacgat ttaatatcgc cacccgcaca aaagaattta tcgccggttg
     4141 ccgttaattc gactaagcgt aatgccgtgt cattttcaat ttcatttaaa taatggtcga
     4201 gagattcgat catttcttca ttaatagcat gacatttatc ttcacggttc aatgtcaggc
     4261 ggcaagaagc cgccgctctg ctgaacagaa cgggttggtc attcatgatc attccttaac
     4321 aataatgcgc gatgcgatcg acgctaatat ccagcgtgct gttaatatct ttctgccacc
     4381 agtacggtga gaatatttcc agttcaatag cgccattaaa tccggcgttt tcaaccagcc
     4441 gacgaattga cggaatattg atgacaccat ctcccggcat tccacggtca ttgaccagat
     4501 cggtggtcgg cactaaccag tcggaaacat gaaacgcgag gatgcgtttt ccggcacgca
     4561 ggatctgact ggcgagatcc gggtcccacc agacatggta gacatctacc gccacgccga
     4621 ggccaaattc gccatcggga tcgagttcat cacaccagtc cagcgcctga cgtaacgtgc
     4681 acagacaaga acgattggca gcagtcatcg ggtgcagtgg ctccagtgca attggcacgc
     4741 cgacctcttt cgaatgtggc agcaactggc gaatcccttg tttcacctgt tcccgtgctt
     4801 cgtacagatc ttttgttccc attggcagac cgccaaccac ctgcatataa caggcggcgt
     4861 tcagtacagc ggcgtcgtcc agcgcccggc ggttatcgtc aatcgccagt ttgcgctccg
     4921 ccagcgtcgg tgcggtgtag taggtgctac ggcacaaacc ggaaacgttc atgttactgg
     4981 cagcaagctg gcgggcggtt tgctggagat cttcactctg caactccctg cgccaggggg
     5041 caatcgcgcc aataccgcgt gcggcgcagg catcaataat ggcgggtagc ggcgttttat
     5101 agcccagtgt cgcggtattg atactgaata aatccgggcg ttgtaacaga tctctcatga
     5161 ttcagcctca gaagcgcggc atacttaagc cgccgtcgat ggcgaccgcc tgaccacagg
     5221 tgtaaataag tttaccttcc gccatcgcgc ggacagtgga agcaatatcc gccggataac
     5281 cccaacgtcc ccacggcacc agacctttgg caatcagttc gtcgtaatag gccgttgccg
     5341 gaatagtcat atcggttttg atgagaccag gacgcacttc atatacgcca atttgttcat
     5401 tacaaaggcg agcagcaaac agacgagcag cggcagagac ggcagttttg gcaatggtat
     5461 attcgccgcg attcatcgcc agcatgatgg cgttgatgga actgacaaaa ataatcgagc
     5521 ggtgcggcag ttctgcttcc ggtttcggct gtgccagtaa gcgcttactg aatgcctgag
     5581 caaggaagaa cggtgcgcgg gtgttaatgg caatattttg gtcgaagctg tctggttcga
     5641 ggtcgagaag gtcaccgcgt tttttcactg aaatgcctgc gttgttgagc aggcagtcca
     5701 gacgtcccca ctggttttgc gctgcgtcga gcatctcttc atgcagggag agatcgccca
     5761 catcagcagg gaagcaaatc acttccacgc cttccgccag acattcttgt tgtgtggcat
     5821 gtaatttttc tacgctatcc gcatccggac gatcattaat cagcagatta aatccgccac
     5881 gcgcaagctc cagcgcacag ccctttccaa taccacgagc ggctccggta atcacggcaa
     5941 caggacgagt ggatttcata caatagctct ctttttttga attttgtttc cttagtgatg
     6001 attagcttgc gactggagga taaaaagatc aaccatctag ttggttgata tgcgatggcg
     6061 atcacaggac atttgataac ctgatgaaag tacaagtttt tgcccttttt tctgaagctg
     6121 aaatcaaatc aggttatgat agtcaccacg aagaggtcgt ggggaagcgt tatgtatctt
     6181 gataccagca acgtacagag cctgaaagaa aagctgctgt tatgcgcggt taatgaattt
     6241 gccgaatatg gttacgaagg ggccagagtc gataatatcg tcaaggccgc ggggtgcagt
     6301 aaacagacgg tttatcacca ctttggtaat aaagaaaatt tatttatcga agtactggaa
     6361 tatacctgga acgatattcg acaaaaagaa aaggcccttg atttctcaga tcttccgcca
     6421 cagaaagcca ttgagaaaat aatcgatttt acatgggatt attatatttc taatccctgg
     6481 tttttaaaaa ttgtgcatag tgaaaatcaa agtaagggtg tgcattatgc aaaatcacag
     6541 cgtcttttag aaattaacca tgcgcacttg cagttaatgg aatccttatt ggatgaaggg
     6601 aagaaacaaa atatttttaa gccagatatc gacccattgc aggtgaatat taatattgct
     6661 gcgcttggcg gatattattt gatcaaccag catacgcttg gcctggttta tcatatcagt
     6721 atggtttccc cccaggcact ggaagccaga cgtaaggtca tcaaagagac aatccttagc
     6781 tggcttttgg ttgacccttc atctaccgcg cacgaataaa actgccatcc ggctggcggg
     6841 tgaacaggac ctgttgatta ttccccgtat caatggttaa gcccgtcacc acgccgctgg
     6901 cgttctggcg gattttcacc atttgcccgt tttgcaaatt actcagcggc ttacccgcgc
     6961 cttctacctg cgccattgca tagacatcgg tagcgggcag tccgtggtcg cggaacagct
     7021 gcgccatcgt tttaccgggt tcgacacggt aagagcgcca ttgattatca attccgctat
     7081 ctggctggaa tggttgtgtt tgggtagttt gcggctgttc ttctggctgg ctttcctgta
     7141 taggttctgg agcgaccgga gccacctgat caggatcatt ttgtggtgtg actaattgcg
     7201 cccgcagctg ttcttctgtc ggcggttgtg attgcgactg aatatctaac tgcgcttcac
     7261 gcgtcaccac cggcgcgttg ggcgtatcat cagatggaag cagaaatccg accaccaata
     7321 taatggcggc aataatgatc ccccgacgat gcatcggcgg cagcgggtcc atgaagcgaa
     7381 aattgtccgg cgcgtgccag actttctcca gggttggttt tagttcaaag cgcccgggca
     7441 tggtttcctc ctgctccgcg tcttgttcct caatcatagc ctatgaataa gctaacgcta
     7501 tgatgtccgt ggtaaacccg cctttattat attcatacgg gatattgcta ttgtttcttt
     7561 ttccccggga tttgtcatca ttcctgcgac aaagttttac ccaaagaagt gtggctgata
     7621 tgctgcccgc tacttttata ccctaagaaa ggaaatacga tgaccacccc aacttttgac
     7681 accatcgaag cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca actgagtgaa
     7741 tctggcctgg aagggctgct gccagaagca ctggttgcag gtattgccga tgcgctggaa
     7801 ggcaaacatc cggctgttcc ggttgatgtg gtgcatcgcg cgctgcgtga aatccacgag
     7861 cgcgccgatg ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg tgtgaaatac
     7921 ctggaagaaa acgccaaaaa agaaggtgtg aatagcaccg aatctggcct gcaattccgc
     7981 gtgatcaacc agggtgaagg cgcaattccg gcacgtaccg accgcgttcg tgttcattac
     8041 accggtaaac tgatcgacgg caccgtgttt gacagctccg ttgctcgtgg tgaacccgct
     8101 gaatttccgg ttaatggcgt gatccctggc tggattgaag cactgactct gatgccggta
     8161 ggttctaaat gggaactgac tatcccgcag gaactggcat atggcgagcg cggcgcaggc
     8221 gcatccatcc ctccgttcag caccctggtg tttgaagtcg aactgctgga aatcctctaa
     8281 gcagcgcatt ctgttcccct cgaaccagag gggagcaggc attcagcaat aaacccttca
     8341 gtttgccaaa cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat ttttgtgagc
     8401 tgtttcgcgt tatcaccgtg atatgacact cactttaaac ataaaattaa cattagatct
     8461 aaatcttagt attcatcccg cgtattgtta cctaatatcg atgagtcccg atacagattc
     8521 gtcgtatcat agactgacta aaggccgtag agcctgaaca acacagacag gtacaggaag
     8581 aaaaaaacat ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg gctgaacagt
     8641 cgctacggcg caatctcaca aaccgacata ttcagcttat tgccattggc ggtgccattg
     8701 gtacggggtt gtttatgggg tctggcaaaa cgattagcct tgccgggccg tcgatcattt
     8761 tcgtttatat gatcatcggt tttatgctct ttttcgtgat gcgggcaatg ggggaattgc
     8821 tgctttcgaa tctggaatac aaatctttta gtgacttcgc ttccgattta ctcgggccgt
     8881 gggcaggata tttcaccggc tggacttact ggttctgctg ggttgtaacc ggtatggcag
     8941 acgtggtggc gatcactgct tatgctcagt tctggttccc cgatctctcc gactgggtcg
     9001 cctcgctggc ggtgatagtg ctgctgctga cgctcaatct cgccaccgtg aaaatgttcg
     9061 gtgagatgga gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg ctgattgtcg
     9121 tcggcctggt tatggtggcg atgcactttc agtcaccgac tggtgtggaa gcgtcattcg
     9181 cgcatttgtg gaatgatggc ggctggttcc cgaaaggttt aagtggcttc tttgccggat
     9241 tccagatagc ggttttcgct ttcgtaggga ttgagctggt aggtacaacc gctgcggaaa
     9301 ccaaagatcc ggagaaatca ctgccgcgtg caattaactc tattccgatc cgtatcatta
     9361 tgttctacgt cttcgcgctg attgtaatta tgtccgtgac gccgtggagt tcggtagtcc
     9421 cggagaaaag cccgtttgtt gaactgttcg tgttggtagg gctgcctgct gccgcaagcg
     9481 tgatcaactt tgtggtgctg acctctgcgg cgtcttccgc taacagcggc gtcttctcta
     9541 ccagccgtat gctgtttggt ctggcgcagg aaggtgtagc accgaaagcg ttcgctaaac
     9601 tttctaagcg cgcagtaccc gcgaaagggt tgaccttctc gtgtatctgt ctgctcggcg
     9661 gcgtggtgat gttgtatgtg aatcctagcg tgattggcgc gttcacgatg attacaaccg
     9721 tttccgcgat tctgtttatg ttcgtctgga caattatcct ttgctcgtac ctggtttacc
     9781 gcaaacagcg tcctcatctg catgagaagt cgatctacaa gatgccgctc ggcaagctga
     9841 tgtgctgggt atgtatggcg ttctttgtgt tcgtggtcgt gttgctgaca ctggaagatg
     9901 acactcgcca ggcgctgttg gtcaccccgc tgtggtttat cgcactgggg ctgggctggt
     9961 tgtttattgg taagaagcgg gctgctgaac tgcggaaata accgcattat catgctggat
    10021 ggcgcaatgc catccagctt ttagattact cacccgccag cgcgcgtggg aacagtacat
    10081 tgttttccag gctgatgtga tccatcaggt catcaatcag ttcattaata ccgttataca
    10141 tcgctttcca ggtggtgcag gcttccggcg gcggtgtgac gttattggtg gtgtgtttaa
    10201 tcacttccag cagttcgccc gcttcatcgt gctcgctttc catcacgctg attggtccca
    10261 ttgcctggct gcccatgcct tgtttgatca tcgggaagag gatctgctct tctttcatca
    10321 tgtggctgga aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacgc
    10381 tcggtttgtc ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagct
    10441 gctcgcggtg acgatcgtgg tagcgcacga tgatatggtc aatgatttct gccagcgggg
    10501 cgctacgcca gtctttctca atcggttgtt cagcaagctt tgccagttca gcttcaatga
    10561 cctcaacatc cagttcttta cgtgccgccg cgcgcgccag cgtctgctta ccgccacagc
    10621 agtaatccat atcatattta cgaaacagag ccgaagcgcg agggatagag agcgccagtt
    10681 cacctaaagg ttggtcgcga taagccatag ctgatacctc attcttaata atataagatg
    10741 tattttaaat gcatctttaa ggcaaaagct ataaccctta cgtagcagag gcttatttaa
    10801 ctcactgcca gcgtgtcaac tttctgagac agattcagtt aaaggttttt ctggttttgc
    10861 tctgaccgca atgaccacgc caatcactaa cagcgcgata ccgctcagcg tcattagcgg
    10921 cggcatttgc tggcggagta aaaaggtgta cagcaaacct gccagcgttt cgaaaacaat
    10981 cagcggcccg agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc
    11041 aacccatgag caaagcacgg ctatcgcaac catcagacta ataaacacca gcggacgggg
    11101 gccaaaaggt aaggagaagt ccggcgtttg tatattcagc cagtaacagg cgacgagata
    11161 gccgatgaga gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg
    11221 tttgtcggga ttttcccgca gccagcgggc gttgcgcagg gcataccatg cccagcagac
    11281 cacggaaact aacgctagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag
    11341 tccgtggttt aactcagcaa tattcacact cgccaggccg atgccaatac aaatcagtgc
    11401 cggggcgagt tttccccacg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa
    11461 gacaggaatg accaccggca gggtgccgat aatcatcgtg gaaacaggcg cgccagtacg
    11521 ttgaatggca ctggcaaggc agaagtaata aatgaggttg cccatcatag tgagcatcaa
    11581 ggcggtaagc cagtcccgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat
    11641 gggcagcgca attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta
    11701 ttccggtacg atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc
    11761 gtacagcacg ccgctaatca ttactggcat ccattggtct gtcagaagaa agttcaggaa
    11821 gcagacatga ttgctcccgg catcccgtca ttattagaga ggatgcggga ggatgccact
    11881 ttaattaact agttattaac attaaaaaga tggcttacgc tttcggctaa cgttgtcgtt
    11941 gggcggccaa tcaatttgct aagcgttttg ctgttatcaa acagaccacc tttcgatgcg
    12001 ccaacgtcag aatccgccag catatccgcc agtccgtcgg gcagtccgac gcttttcagt
    12061 gccgcggcga aatcggcttc gctcagattt tgataggtaa cggattttcc gctctgtttg
    12121 gtcagctctg ccgctaactg tgtcaacgtc caggcactat cgcccgccag ttcgtaaacc
    12181 ttgccttcgt gaccggcttc gctaatcacg cgtgccgcag ctgccgcata atctgcccgc
    12241 gttgctgagg cgattttgcc atcgcccgcc gcaccgataa atacgccgtg ttccagtgct
    12301 gccggggcgc tggcgaggta gttttcggtg taccagccgt tgcgcagcag ggtgtaaacg
    12361 atgccagaat cagccagcat tttatccgtc tcgatgtgct catcggcgag gccgagcggg
    12421 gaggtatctg catgtagcag gctggtataa gcgataaatt tcacgccagc cgcctttgcg
    12481 gcattaataa cattacgatg ctgcggggca cgttgaccca cttcgctgga agagatcagc
    12541 agtagttttt ccactccctg aagtgcagat gtcagtgcgg cttcatcgcc gtagtcagcc
    12601 tgacgcacgg taatgccttg tgctgccagg gcctgggctt ttgccggatt acgaacgata
    12661 gccactattt ggctggcagg aaccgttttc atcaaggatt caataacata gtgaccaagt
    12721 tggccagtgg caccagtaat agcgatcatg ggaagtctcc atcgttttgt cttgtgttta
    12781 aatacgctaa cacctaaact tacttttagt aagtacgtac aaaaaggtaa gtatgaaatg
    12841 agtcaggtta gcctgtcgca acaactgaaa gagggtaatc tctttgcgga acagtgcccg
    12901 tcgcgcgagg tgttgaaaca cgtcaccagc cgttgggggg tattgattct ggtggcgcta
    12961 cgcgaaggta ctcatcgctt tagcgacctg cggcgcaaaa tcggtggggt gagtgaaaag
    13021 atgcttgcgc agtcgttaca ggcgttagaa caggatggtt ttcttaaccg tatcgcgtat
    13081 ccggtggtgc cgccgcatgt ggaatacagc ctcacgccgc tgggcgagca ggtgagcgaa
    13141 aaggttgctg cactggcgga ctggattgag ttgaatttgc ccgaggtgtt ggcggtgcgg
    13201 gatgaacgtg cggcataact gccggatgcg ctgagcttat ctggtctacg gtgcatggct
    13261 tgtagggcgg ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcggttgat
    13321 gatttatagg ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac
    13381 ccgatatcat cggtcgcgac tttattcatc ggatattgcc ctttctcttt aataaacgct
    13441 gcggctttat ctgacgggga agtttcgaaa cggatatcca gtttcttatc gccagcaatc
    13501 ggtgctaaac gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca
    13561 tcagcaatcc acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg
    13621 ctgtcgcccg taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg
    13681 aacatggcgt tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca
    13741 ttggcattaa tcatctggca ctcgccgtca taacgggcag gctgggtaac atcaatctga
    13801 taattcacac catcaatcac atcaaagtta taggtgcgga aaccatccca gttgatgagt
    13861 gattgcggtt tcgtgctgtt aggatcaatc tggttaaact gtccggcgga gcactccagc
    13921 cactctttca cctctttacc gctggctttc actacaatca gcgtattggg gtagagataa
    13981 agatcggcgg cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg
    14041 tcatttttgc gaccaccaac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc
    14101 agatccggat cgccctgaat gtaatgttcg acatacgctt tttgcgcgtt gttcactact
    14161 tgcacggtcg gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta
    14221 ccgattggct tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc
    14281 agcttgctgt cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgagct
    14341 tccgctttcg cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc
    14401 acaccaagat gatcgcccca catgcctggc attaccgccg gaacaccatt cagcgtgcct
    14461 ttcgcgatat cagctccttc gatatcagca aaatctttac ttgggaaaac ggcgtgagca
    14521 tggccaaaca taatggcgtc aacacccgga atttcactga gataataaac tgagttttcc
    14581 gccatcactt tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgagatcg
    14641 gcacctttct cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc
    14701 gtcactttcc cggataaatt agctttatcc cagcccatga tttgcggcgg tacgacgcca
    14761 atatagccaa tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta
    14821 attaaatacg gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca
    14881 taagggaatt tcgctcccgc cagcgcattt ttcaggtaat ccagaccgta gttaaactca
    14941 tggttgccaa gcgtgccgac ggtatagtcc agcgtattta atgccttata cactgggtga
    15001 atatcacctg cttttaatcc tttcgccgac atgtaatctg ccagcggact cccctgaatc
    15061 aaatcgccgt tatcgaccag tacgctgttt ttcacttcat tgcgggcatc gttaatcagg
    15121 cttgccgtac gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc
    15181 atcatgttgc tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc
    15241 acactggcgg caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc
    15301 cttttatcat cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg
    15361 aagaatgcca caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt
    15421 aacgataggg tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa
    15481 tgttagatca agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg
    15541 acgggacgaa accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg
    15601 atattgccgc acacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg
    15661 tcctttctga agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc
    15721 tggtagaccc gctggatggt actaaagagt ttattaaacg caatggcgaa ttcaccgtta
    15781 acattgcgct cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga
    15841 acgtaatgta cagcgcggca gaaggcaagg cctggaaaga agagtgcggt gtgcgcaagc
    15901 agattcaggt ccgcgatgcg cgcccgccgc tggtggttat cagccgttcc catgcagatg
    15961 cggagctgaa agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt
    16021 cgctgaaatt ctgcctggtg gcggaaggac aggcgcagct gtacccgcgc ttcggaccaa
    16081 cgaatatttg ggacaccgcc gctggacatg ctgtggccgc agctgccgga gcgcacgttc
    16141 acgactggca gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatcctgggt
    16201 tcagagtgtc tatttattaa ttaatttatg cagcaggtcc atcacttgct gcacctcttc
    16261 ctgagtaagc gccccgtctt tggcccattg cacgcgaccg tctttatcca gcaccactac
    16321 agcggaactt ttttcatcca gctgccaggc accgcgtgcg acgccattgc tatcaacaat
    16381 aaactgcgac cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc
    16441 ggaacccgga attgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg
    16501 taacttcgct gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg
    16561 accagcaata tgttgcagta ctcgcacttt tcccactaac tgcgcgctgt tccaggtttt
    16621 gtagctaaac tgatctttat caagcaccaa ctcgccacga tcggtgatgc caatcggcgg
    16681 cactcgctga ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt
    16741 gagtgccaga atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg
    16801 accacttggg tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat
    16861 gccagtttgc ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagaccgc
    16921 gcttcggtaa aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg
    16981 gtggaaacat tgcccggata gtctatagtc actaggcatt aaaatttgcg cctcataata
    17041 gttgggccga ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt
    17101 ttccaacggt acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg
    17161 ttatacatca aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa
    17221 gtgaaggata agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg
    17281 caaacagaga tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag
    17341 ccgttgatgt ttctgggggt tacgcgacgc tttcttcttt atctttagcg tcaggcagtt
    17401 ttggcgaaag ggcggtggcc ttgctgtcga tccgggtcac cagcagctgg tcgatgcggt
    17461 agttatcgat atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt
    17521 tcgggatctt acgcagcata aacatcataa agcctccgat ggtttcgtag ttgcccgact
    17581 gcgggaactc gtcaatatcc agcacgcgca tgacgtcgtc aattggggtg ccgccgtcaa
    17641 tcagccatga gttctcatca cgggcgacaa tctgctcttc cagcccctga ccgaccagat
    17701 cgcccatcag cgtggtcatc acgtcgttga gggtgatgat ccccaccacc agcgcgtact
    17761 cgttcatgat caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa
    17821 gggttaacgt atccggcaca atcagcgtgt tgcgaatttg tacgccgctg ttcagtgcca
    17881 ggctttggtt agccagcacg cggttcagca ggtctttaga atcgacataa ccgatgatgt
    17941 ggtcaatatc ttcattacag acgaggaact tagagtgcgg atgttccgcc accttattct
    18001 tcaggctttg ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg
    18061 aagacggaac ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt
    18121 tacgtaacac gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga
    18181 tgtcatcttt acgtaccatt ggcagtttga aaatacggaa gatcatgttc gccaggccgt
    18241 tgaagaacca caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac
    18301 gcaaagccac cgcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca
    18361 ggataaacat gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagtt
    18421 ctgccgacat atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga
    18481 cgataccgcc gagaatcgca actgcgttca gaccgatttg gaccaccgta aagaacatgc
    18541 cgggattttc ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca
    18601 gtttaagttt gattttgcgt gaggcggcaa gtgagatctc agacatcgag aagaacgcac
    18661 ttacagcgat caagcagagt atgactaaaa tactgtttaa catatcttat ccgacctttc
    18721 aggccagatc ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga
    18781 ttcgaagaaa gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagtcg
    18841 ccagagggtt aaaattcaga ctgctgataa atgcaatact gcctgatgcg ctatgccgat
    18901 caggcctacg cagtccctgc aatatattga atttggacga ttttgtaggc ctggtaaggc
    18961 attcacgccg catctggcat aaataaagcg cactttatca acaatctaca tttgtacccg
    19021 taacgctatg cttccggtgg cagacagacg ccaattccgc caattccaca gtaaccatac
    19081 gggtttttat gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta
    19141 gcgatttccg tggtgatgtg acgatcgtca tcggcggcaa gcatcgccgc ctgaaaacgt
    19201 tccagactgg cgcgagctgc ggcatcctgt tctggggtca gcggataaat cgctgaacga
    19261 tactgcgtgc cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa
    19321 aatacctgta gcaactgctc atagctgatg acggaaggat cgtaaacaat gcgtaccgct
    19381 tcggcatgac ccgtatcacc ggagcacact tcccgataag tcggatttgg cgtatagcct
    19441 ccggtatagc ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccact
    19501 ccccagaaac aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt
    19561 gagtgaccgt tgaccgcatg cagcgtggct acgggcatcg gggtgttacg tccaggcagg
    19621 gcatcggcgg gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga
    19681 aaatcggtca ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt
    19741 gaataggtct aaagtaaaac ataagaaata tttggggttt agtctgcttt ttaatccata
    19801 ttactggatt tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca
    19861 ggagaaaatg tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga
    19921 tctgccgtcg ccgcgaacgt ccgtctacag gtcgaggggt tatcgggaca gctggaaaag
    19981 aacgttcgtg cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt
    20041 cgcgcacgcg tcgatgatgc catccgcgaa ggtctgaaag cgctgggtta ttaccagccg
    20101 accattgaat ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa
    20161 gtcacgccag gtgtgccggt gttaattggc ggcaccgatg tggtattgcg cggcggcgcg
    20221 cggaccgata aagactattt gaaattgctc gatactcgcc cggctattgg cacggtgctg
    20281 aaccagggcg attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt
    20341 tatttcgata gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagcc
    20401 ttctgggata ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa
    20461 ggatcacaaa tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag
    20521 tacgaatcga aagatctggc ggaactgaac cgccgacttt ctgctaccgg ctggtttaac
    20581 tcggtggtgg tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt acccttgacg
    20641 ggcgtggttt cgccgcgaac tgaaaacacc atcgaaaccg gggtcggtta ctctacggac
    20701 gtgggaccgc gcgtgaaagc gacgtggaaa aagccgtgga tgaactctta tggtcacagt
    20761 ctgaccacca gtaccagtat ttccgcgccg gaacagaccc tcgacttcag ctataaaatg
    20821 ccgctgctga agaatccact ggaacaatat tatttggtgc agggcggttt taagcgcact
    20881 gacctgaacg ataccgaatc tgactccact acgctggtgg cttctcgcta ctgggatctc
    20941 tccagcggct ggcagcgtgc cattaacctg cgctggagcc tcgaccactt tacccagggt
    21001 gaaattacca acaccacgat gctgttttat cctggggtga tgattagccg cacgcgttct
    21061 cgtggtggcc tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac
    21121 acggcctggg gttcagatgt cgatttctcc gttttccagg cgcagaacgt ctggatccgc
    21181 acactgtacg atcgccatcg tttcgttaca cgcggcacgc tgggctggat tgaaaccggt
    21241 gatttcgaca aagtaccgcc ggatctgcgt ttcttcgccg ggggcgatcg cagtattcgc
    21301 ggctacaaat acaaatctat cgccccgaaa tacgccaacg gtgacctgaa aggggcctcg
    21361 aagttgataa ccggatcgct ggaataccag tacaacgtga ccggaaaatg gtggggcgcg
    21421 gtgtttgtcg atagtggcga agcggtaagc gatattcgcc gcagcgactt taaaaccggt
    21481 accggggtcg gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta
    21541 ccggtcgcgg ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta
    21601 tgagtttatg gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat
    21661 cggtggcgtt tctggtgggc accaccagcg gcctgcatct tgtatttaaa gcggcggatc
    21721 gctgggtgcc aggactggat attggcaagg tcaccggcgg ctggcgcgat ctcaccttgt
    21781 ctgacgttcg ttatgagcag ccaggcgtgg cggtaaaagc gggtaatctg catctggcgg
    21841 tcggtcttga gtgcctgtgg aacagtagcg tttgtattaa tgacctggcg ctgaaagaca
    21901 ttcaggtcaa catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag
    21961 atagcggtcc gctggatctc tccacgccgt atcccatcac cctgacacgg gtggcgctgg
    22021 acaacgtcaa catcaagatt gatgacacca cggtgtcggt gatggacttc acctccggcc
    22081 tgaactggca ggagaaaacc ctgaccctga aaccgacgtc gctgaaaggc ctgctgattg
    22141 ctctgccgaa agtggcggaa gtggcgcagg aagaagtggt cgaaccgaaa attgaaaatc
    22201 cgcagccgga ggaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat
    22261 tgccggaaat gaccgacgtg catttgccgc ttaacctgaa cattgaagag tttaaaggcg
    22321 agcagttacg cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga
    22381 gcagcattga cggcaatact aaactggacg ccctggatat cgattccagt caagggatcg
    22441 tcaacgccag cggcacggcg cagctgtcag acaactggcc ggtggatatc accctcaaca
    22501 gcacactgaa cgtggagccg ttgaaaggag aaaaactgaa gctgaaagtg ggcggtgcgc
    22561 tgcgcgaaca gctggagatt ggcgttaacc tttccggtcc ggtggatatg gatttacgcg
    22621 cccagacgcg actggcggaa gccggattgc cgctcaacgt ggaagtgaac agcaaacagc
    22681 tttactggcc gttcactggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta
    22741 ccggcaaaat gaccgattac acgctctcta tgcgtacggc agtgaaggga caggagatcc
    22801 cgccagccac cattaccctt gatgccaaag gtaatgaaca gcaggtcaat ctcgacaaac
    22861 tcaccgtcgc ggcgctggaa gggaaaactg aactcaaggc gttgctcgac tggcagcagg
    22921 ccattagttg gcgcggtgag ctaacgctta atggcattaa caccgccaaa gagttcccgg
    22981 agtggccgtc gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct
    23041 ggcagatgga ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg
    23101 ttgacggcac gctgaaaggc aacagttata tgcagtggat gatcccaggg ctgcatctgg
    23161 aactcgggcc aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg
    23221 atgccaccat caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga
    23281 aagggctggt gaaagtacgc ggcacggtgg aagcgccaca actactggca gatatcaccg
    23341 cgcgcggcct gcgctggcag gaactttccg tggcgcaggt tcgcgtggaa ggcgacatta
    23401 aatccaccga tcagatcgcc gggaaactcg acgtacgcgt tgagcaaatt tcgcagccgg
    23461 atgtaaatat caacctcgtc accctgaatg ccaaaggcag cgaaaagcag cacgagctac
    23521 agttgcggat tcagggcgag cctgtctccg ggcagcttaa tctggcagga agttttgatc
    23581 gcaaagaaga acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc
    23641 cgtggtcgct gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca
    23701 tcgggccaca ctgctggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg
    23761 cgggggccga agggcgtgcg gtggtgaatc tcaaccgctt cgacctcgcc atgctgaaac
    23821 cgtttatgcc agaaaccact caggccagcg gtatcttcac gggtaaagcg gatgttgcct
    23881 gggacaccac gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc
    23941 aggtaacgca aaccgtcaac gatgcggcgc tgcccgtggc gtttcagaca ctgaatctga
    24001 cggcggaatt gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg
    24061 gccagtttga tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca
    24121 acgtcaatat ccgtaacttc aaccttgcga tgataaaccc catctttaac cgtggggaaa
    24181 aagcagcggg gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt
    24241 tgtttggtca gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata
    24301 tgcagccgag ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gccggtacag
    24361 tacggaccca gcagggtgaa atctacctga acggtgatgc cgactggagc caaattgaaa
    24421 actggcgggc acgagtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg
    24481 tacgaatgga tgtatcgcca gatgttgtat tcgaggctac accaaacctg tttaccctcg
    24541 atggtcgcgt ggatgtcccg tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag
    24601 taggcgtctc cagcgatgtg gtgatgctta acgataacct gcaaccggaa gagccgaaaa
    24661 cggcgtcgat tccgattaac agtaacctga ttgtccacgt tggcaacaat gtgcgcattg
    24721 acgcctttgg cctgaaagcg cggctgacgg gcgatcttaa cgtcgttcag gacaaacaag
    24781 ggctgggcct gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg
    24841 atctgattgt gcgtaagggt gagttactgt tctctggtcc gccggatcaa ccgtatctta
    24901 atattgaagc tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagttcgcg
    24961 tcactggtct ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac
    25021 aagctgcatt gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg
    25081 cggcaatgac ctcgatgctg attggtctgg gggttgcgca aagtggccag attgtgggta
    25141 aaatcggcga gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact
    25201 cctcccaggt agtagtcagc ggctatgtat tgccaggtct gcaagtgaaa tacggcgtgg
    25261 gtatatttga ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc
    25321 tggaagccgt gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc
    25381 aatgcgaata tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac
    25441 caatgcccag ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca
    25501 ctatccaggc gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgacaa
    25561 cgccacgctg gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt
    25621 gattcagacg ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt
    25681 aaagctaatt gaaagcggcg actggttaga cagggataag taaccatatg catacgccac
    25741 cttcgggtgg cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcaccc
    25801 tttgcttttc tctccgagcc gctttccata tctgttaacg cataaaaaac tctgctggcg
    25861 ttcacaaatg cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac
    25921 ttaaggaggt gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca
    25981 ttcccaatat cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaggcgcaag
    26041 tgagcaacaa tcaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc
    26101 tggcacagtg tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca
    26161 cccctgcggg tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct
    26221 ggttggcttt gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt
    26281 gctctccgtt caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg
    26341 cggaaatttt gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt
    26401 gcacggcgtg gtgctggtga atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa
    26461 gcgtattggt ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt
    26521 ggtggaataa ggtgtgttta tttatcgcgg gcataaaaaa acccttacta accgaagctc
    26581 ggcgttcagg gttattgcgc tagaagaact tatttattct ttgcgcgctc gaaggaagca
    26641 acgatttcag ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct
    26701 ttttcgaggt ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggc
    26761 agatcgttaa cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca
    26821 accagtttcg catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa
    26881 cggatcacag aacccggctg cagcgggtac ggagtcggga ccagtacgtc aaccgggtca
    26941 ccgtccagag acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg
    27001 gacatgaagc ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga
    27061 tctgcgttag ccgggatctc aataaccacg tagatgtctt ccggcagatc tttacccgca
    27121 gggacgttga gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag
    27181 tattatagcc aactcgcgcc gaatgtcttt gcttgttttc gtcttcattt tcctttttcg
    27241 ccagttttaa gacggtatac agagcaggaa aatccataac cgtagccgca tttttcatag
    27301 tgagatgaaa gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat
    27361 gtaacgcaat ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca
    27421 ataataatta ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag
    27481 tctcggcagc catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg
    27541 tcggatcgga atccggctgg cgcgctgcag aaaccaatgt ggcgaaaagt gaggccgaaa
    27601 aacgcggaat tacgttgaaa attgccgatg gtcagcaaaa gcaggaaaac cagattaaag
    27661 cggtacgttc cttcgttgca caaggggtgg atgcgatctt tatcgctcca gtggttgcga
    27721 cgggttggga accggtatta aaagaggcga aagatgccga aatcccggtc ttcttgcttg
    27781 accgttccat cgatgtgaaa gacaaatctc tctatatgac caccgtcact gccgacaaca
    27841 tcctcgaagg caagttgatt ggtgactggc tggtaaaaga agtgaatggc aaaccatgca
    27901 acgtggtgga actgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct
    27961 ttgccgaagc cattaagaat gcgccaaata tcaaaatcat ccgctcgcag tcaggtgact
    28021 tcacccgcag taaaggcaaa gaagttatgg agagctttat caaagcggaa aacaacggca
    28081 aaaacatctg catggtttac gcccataacg atgacatggt gattggcgca attcaggcaa
    28141 ttaaagaagc gggcctgaaa ccgggcaaag atatcctcac gggttccatt gacggtgtac
    28201 ccgatatcta caaagcgatg attgatggcg aagcgaacgc cagtgttgaa ctgacgccga
    28261 atatggcagg tcccgccttc gacgcgctgg agaaatacaa aaaagacggc accatgcctg
    28321 aaaagctgac gctgaccaaa tccacccttt atctgcctga taccgcaaaa gaagagttag
    28381 agaagaagaa aaatatgggg tattgagggt tgctatgcct gatgccgatt cgtaggccgg
    28441 ataaggcgct cgcgccgcat ccggcgatgg tgcactgaag cctgatgcga cgcttaccgc
    28501 gtcttatcat gcctactggg agcacgcttt acaccggggg aaaccatgac gaccgaccaa
    28561 caccaggaga tcctccgcac cgaaggatta agtaaatttt tccccggcgt caaagcgtta
    28621 gacaacgttg atttcagcct gcgccgtggc gaaatcatgg cgctgcttgg tgaaaacggg
    28681 gcgggaaaat caacgctaat caaagcatta actggtgtat accacgccga tcgcggcacc
    28741 atctggctgg aaggccaggc tatctcaccg aaaaataccg cccatgcaca acaactcggt
    28801 atcggcaccg tctatcagga agtcaacctg ctacccaata tgtcggttgc tgataatcta
    28861 tttataggcc gcgaacccaa acgtttcggc cttctacgcc gtaaagagat ggaaaagcgc
    28921 gccaccgaac tgatggcatc ttacggtttc tccctcgacg tgcgcgaacc gcttaaccgt
    28981 ttttcggtgg cgatgcagca aatcgtcgcc atttgccggg ctatcgatct ctccgccaaa
    29041 gtgctgatcc tcgatgaacc caccgccagc ctcgacaccc aggaagtgga gctattattt
    29101 ggcctgatgc gtcagttgcg cgatcgcggc gtcagcctga tctttgtcac tcactttctc
    29161 gatcaggtct atcaggtcag cgatcggatc accgtcttac gcaacggcag tttcgtaggc
    29221 tgtcgggaaa cgcgcgagct accgcagatc gaactggtaa aaatgatgct ggggcgcgag
    29281 ctggataccc acgcgctaca gcgtgccggg cgaacattgt tgagcgacaa acccgttgcc
    29341 gcgttcaaaa attacggcaa aaaaggaacg atagcaccgt ttgatctcga agtgcgcccc
    29401 ggcgagatcg tcggtctggc tggcttgctg ggatccggac gtaccgaaac cgccgaagtg
    29461 atcttcggta tcaaacctgc tgacagcggc acggcgttga tcaaaggcaa accgcaaacc
    29521 ctgcgatcgc cacatcaggc ttcagtactg ggcatcggat tctgcccgga agacaggaaa
    29581 accgatggca tcatcgctgc cgcctcggtg cgggaaaata tcatcctcgc tctccaggcc
    29641 cagcgcggct ggctacgacc catttcccgc aaagagcagc aagagattgc cgaacgcttt
    29701 atccgccagc ttggcattcg cacaccttca actgaacaac cgattgaatt tctctctggc
    29761 ggcaatcagc aaaaagtgtt gctttcacgt tggctactga cccgaccgca atttctgatc
    29821 ctcgacgagc caacccgcgg cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt
    29881 gaaacgctgt gtgctgacgg tctggcgctg ttggtgatct cctccgaact ggaagagctg
    29941 gtgggctatg ccgatcgggt gattatcatg cgcgatcgca aacaggtggc ggagatcccg
    30001 ctggcagagc tttccgttcc ggcgatcatg aacgccattg cggcgtaagg agaacagtgt
    30061 gatgcctcaa tctctcccgg acactacgcc gccgaaaagg cgctttcgct ggccaacggg
    30121 aatgccgcag ctggcggcgc tattgctggt gctgctggtc gatagcctgg tagccccgca
    30181 tttctggcag gtggtgcttc aggatgggcg tttgttcggt agccctatcg acattcttaa
    30241 ccgtgcagct cccgttgcgt tactggcgat tggtatgacg ctggtgatcg ccaccggtgg
    30301 gattgatctc tccgtagggg cggtgatggc tatcgccgga gccacaacgg ctgcgatgac
    30361 ggtcgcggga ttcagcctgc cgattgtttt gttaagcgcc ctgggcaccg gcatcctggc
    30421 gggattgtgg aacggcatac tggtagcgat cctcaaaatt cagccgtttg tcgccaccct
    30481 gatcctgatg gtcgccgggc gcggcgtggc gcaactgatc accgccgggc agatcgtcac
    30541 gtttaactcg ccggatctct catggtttgg cagcggatcg ctgttgttcc tgccaacgcc
    30601 ggtcattatc gcggtgctga cgcttctcct gttctggctg ttgacccgca aaacggcgct
    30661 ggggatgttt atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa
    30721 cacgcggatc atcgtcatgc tcacctacgt gttgagcggg ctgtgtgcgg cgattgcggg
    30781 cattatcgtg gcggcggata ttcgcggtgc cgatgccaat aacgccgggt tatggctgga
    30841 gctggacgcc attcttgcgg tggtgattgg cggcggatcg ctgatgggcg ggcgctttaa
    30901 cctgctgctc tcagtcgtgg gggcgctgat tattcagggg atgaacaccg gaattttact
    30961 ttctggcttt ccgccggaga tgaaccaggt cgtaaaagcg gtggtggtgc tttgtgtgct
    31021 gattgttcag tcgcaacgct ttatcagtct gattaaagga gtacgtagcc atgataaaac
    31081 gtaacttgcc gctgatgatc accatcggcg tctttgtgct gggctatctc tactgcctga
    31141 cccagtttcc cggttttgct tccacaagag tgatctgcaa tatcctgacc gataacgcct
    31201 ttcttgggat aatcgccgtt ggcatgacct ttgtgatcct ctctggcggt atcgatctct
    31261 ccgtcggttc ggtgatcgcc tttactggcg tgtttctggc aaaagtgatt ggcgatttcg
    31321 gcctctcgcc gctgctggcg tttccgctag tgctggtgat gggctgtgcc tttggcgcat
    31381 ttatggggct tttgatcgac gccctgaaga tcccggcatt tatcattacc ctggcaggaa
    31441 tgttcttttt gcgcggcgtc agctatctcg tttcagaaga gtcgatcccg ataaaccatc
    31501 ccatttatga cacgctctca agccttgcgt ggaaaatccc tggcggcggt cgcttaagtg
    31561 cgatgggact gctgatgctg gcggtggtgg ttatcggcat attcctcgcg catcgtaccc
    31621 gttttggtaa tcaggtatac gccattggcg gcaacgcaac gtcggcaaac ctgatgggga
    31681 tttctacccg cagcaccact attcgcattt atatgctctc caccggactg gcaacgctgg
    31741 cggggattgt cttctcgatt tatacccagg ccggatatgc gctggcgggc gtaggtgtgg
    31801 aactggatgc tatcgcctca gtggtaattg gcggtacgct tttgagcggt ggcgttggaa
    31861 cggtattagg gacgcttttt ggcgtggcga ttcaggggct aattcagact tatatcaact
    31921 ttgacggtac gctgagttcc tggtggacga aaatcgccat cggcattttg ctgttcattt
    31981 ttattgcctt acagcgtgga ttaacggtgc tgtgggagaa tcgtcagagt tcgccagtga
    32041 caagagtcaa tattgcgcag cgataaaaac gcctctccgt gtggagaggc gcaggagatt
    32101 acgcgtccgg gaattcacgg ataaagcgtt cgacatcttc aaccatatgg tcgttgccga
    32161 caaagaacga acggcgctgg tgcagagttt ccgggatgat atccagaata cgctctttgc
    32221 catcgctcgc tttaccgccc gcttgttccg ccaggaatgc catcgggttg cactcataca
    32281 gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg gtagagataa ataccgcctt
    32341 tcagcaggtt acggtggaaa tccgcgacca gtgaaccgat ataacgtgag gtatacgggc
    32401 ggttggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc ccgttcggaa
    32461 acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg aagcgcatcc
    32521 gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg tgaacaccgc
    32581 atccggtggt gtaaaccagc atggtagagg agccgtatac cacgtaacct gccgcaacct
    32641 gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc
    32701 ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg gacgagccat
    32761 ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag acgacaatct
    32821 catcttcttc ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt gcggctttca
    32881 gtttttcatt agcgaacaag tcgagtttct gctgaacctc gccctgcacg ttctcagcac
    32941 cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg ataatcttgg
    33001 cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga gaaaactcgt
    33061 gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg caatgtttat
    33121 ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg
    33181 cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa
    33241 atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta ggaatttgtg
    33301 gcacatttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt
    33361 cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc attgagctga
    33421 ttcagggtta cgatgccagc cagctcgatc cgcagccgga tctggtgatt attggcaacg
    33481 ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc ccttatatgt
    33541 caggtccaca gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg gccgttgccg
    33601 gtacacacgg caaaactacc accgcgggaa tggcgacctg gattctggaa cagtgcggtt
    33661 acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg gcgcgtctgg
    33721 gcgaaagcga cttctttgtt atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac
    33781 gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt gagttcgatc
    33841 acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat ctggtgcgta
    33901 tcgttccggg gcagggccgt attatctggc cggaaaatga catcaacctg aaacagacca
    33961 tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac tggcaggcga
    34021 aaaagctgac cactgacgct tctgaatggg aagtcttgct ggatggcgaa aaagtgggcg
    34081 aagtgaaatg gtcgctggtg ggcgaacata atatgcacaa tggcctgatg gcgattgcgg
    34141 cggctcgcca tgttggtgta gcgcctgcag atgccgctaa cgcgctgggt tcgtttatta
    34201 acgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtgacggta tatgacgatt
    34261 ttgcccatca cccgacggca attctggcaa cgcttgcggc gctgcgtggc aaagttggtg
    34321 gtacggcgcg cattattgct gtgctggagc cgcgctcgaa taccatgaaa atggggatct
    34381 gcaaagacga tctggcacct tcattaggtc gtgccgatga agtcttcctg ctgcaaccgg
    34441 cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct gcacactgga
    34501 gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct ggcgaccata
    34561 ttctggtcat gagcaacggc ggttttggtg ggatccatca gaaactgctg gatggtctgg
    34621 cgaagaaggc ggaagctgcg cagtaattcg gcctcagcct gagatagcat tgccggataa
    34681 ggcgtttacg ccgcatccgg catttgagca tagtgcctga tgcgacgctt gatgcgtctt
    34741 atccggccta caggtgcacc gcatccggca aaccatactc acatcaacaa cgaaaattac
    34801 ccttcattct ccgccagttc gcgcagatac tggaaaatct ggcgtgcgga tttcggcggc
    34861 ttattccctt ctttctcttt cttcgcgtta cggatcagag tacgcagctg ctgacgatcg
    34921 gcatcgggcc acagattcaa tacttcagcg atggcgtcat caccttgatc gatcagacga
    34981 tcgcgcaggt tttccagttt atggaacagc accacctgct ggttgtgacg gtttttcagc
    35041 ttatccagcg cctgacgaat aggctctacg tcgcgctggc gcagcatctt accgatgagc
    35101 tgcaactggc ggcggcgacc ttccatctta atacgctggg cgagctcaat agccgcgcgc
    35161 agatccgcat ctaacgggat cttatccagc gcgtttttcc ccagatcaac aatttccgcg
    35221 ccaaggcgtt ttagctcctc ggcatcacgt ttaatttcac ttttactgac ccagataatt
    35281 tcatcgtctt cgtcttcgat gtcatcaccg ggaacgtcgt cgagccagtc ttcgggctgc
    35341 ttagtcatct caggctcctt aaaaaaagag gctaatgtta ccagttaaga tgcgcactga
    35401 aaaacggttc tctgttagac ttcagagaaa ctctctacat tatggcactt gcaatgaaag
    35461 taatctctca agttgaagcg cagcgcaaga ttctggaaga agcagtttcc actgcgctgg
    35521 agttggcctc aggcaaatcg gacggtgcgg aagttgccgt cagcaagacc accggcatta
    35581 gcgtaagcac gcgttatggt gaagtggaga atgttgaatt caatagcgat ggcgcgctgg
    35641 ggatcactgt ttatcaccag aaccgcaaag gtagcgcatc atccaccgat ttaagcccgc
    35701 aggccattgc ccgcaccgta caggcggcgc tggatattgc ccgttatacc tcgccagatc
    35761 cctgtgccgg cgtggcagac aaagagctgc tggcctttga cgcaccagat ctcgacttgt
    35821 tccaccctgc ggaagtttcc ccggatgaag ccattgaact ggcggcccgc gcagaacagg
    35881 cggcattgca ggcggacaaa cgcattacca ataccgaagg cggcagtttt aacagccact
    35941 acggtgtcaa agtttttggc aacagccacg gcatgttgca gggttactgc tctacgcgtc
    36001 attcactctc cagctgtgta attgccgaag aaaatggcga tatggagcgt gattacgcct
    36061 acaccattgg ccgcgcgatg agcgatctgc aaacgccaga gtgggtcggg gccgactgtg
    36121 ctcgccgtac tttatcgcgc ctgtcaccgc gtaaactctc caccatgaaa gcgccagtca
    36181 tttttgccaa tgaagtggca accgggcttt ttggccatct ggtgggggcg atagcgggtg
    36241 gctcggttta ccgtaaatct acctttctgc tggattcgct gggtaaacaa attctgccgg
    36301 actggctgac cattgaagag catccgcatc tgctgaaagg gctggcgtcg acgccattcg
    36361 acagcgaagg tgtgcgcacc gagcgtcgcg atattattaa agatggcatc ctgactcagt
    36421 ggctgctgac cagctactcg gcgcggaaac tggggctgaa aagcaccgga catgcgggcg
    36481 gtattcacaa ctggcggatt gccggacaag ggctaagctt cgaacaaatg ctcaaagaga
    36541 tgggcaccgg gctggtggtg acggagctga tgggccaggg cgtgagtgcc attaccggtg
    36601 attattcccg tggtgcagcg ggcttctggg tagagaatgg cgaaattcag tatcccgtta
    36661 gtgaaatcac catcgcaggt aatttaaaag atatgtggcg caatattgtc accgtcggta
    36721 acgatattga aacacgcagt aatatacagt gtggttctgt gctgttgccg gagatgaaaa
    36781 tcgccgggca gtaaaaaact ggcgcgacct gccgcgctat aaactaaaaa attacacaaa
    36841 atcattcgca ctgcatcgag acggaaagtg aatgagggcc ggggagcgta ctcgcagtac
    36901 gtgacccggg cgaatgagcg tagccgtcga tgaggcagtg tgaaggatga agtgtaaata
    36961 aaaaaggaag tgagcaatgc gtaaaagcct gttagctatt cttgcagtct cctcgttggt
    37021 attcagttct gcgtcgtttg ccgctgatct tgaagacaat atggaaaccc tcaacgacaa
    37081 tttaaaagtg atcgaaaaag cggataacgc ggcgcaagtc aaagacgcgt taacgaagat
    37141 gcgcgccgca gcgctggatg cgcaaaaagc aacgccgccg aagctcgaag ataaatcacc
    37201 ggacagcccg gaaatgaaag atttccgcca cggtttcgac attctggtcg gtcagattga
    37261 cgacgcgctg aagctggcaa atgaaggtaa agtaaaagaa gcgcaggctg ctgcagagca
    37321 actgaaaacg acccgcaacg cctatcacca gaagtatcgt taattcctca tttccctgtt
    37381 gcctgcactc aggtaacagg gaacttatca tcgcaaatga tgcaccacct gattgctgct
    37441 gccgcgccag atcagggaag ggtcttttaa atcctgcaca aatttgccgt cgaccagtac
    37501 gttaatcaaa tcaacaacct gcatttgcgc agcgttgagt tcgtcgagtt tatagcctgt
    37561 ccacacccag atgtctttac ccggacactc ggcgcggatg cgttttacca gtttcagaat
    37621 atccggcacg ttttgcggat gcagcggatc gccgccggag agggaaatac cctggcgttt
    37681 gatacgagtg tcattcagat cgttaataat ctggtcttcc attgctttag taaatgtctg
    37741 accggaattt acccgccatg tgcttttgtt atagcaaccg gggcattcat gaacacaccc
    37801 ggagacaaac agggtgcagc gagtgccggg gccgttgacg atgtcgacag gatagtactg
    37861 atgataattc attgtttcgc ctggcttatc agtccgcgta taggatgcat ccggtaatca
    37921 acgcctgatg cgacgctgtc gcgtcttatc agacctacaa tcgttgttgc catgtaggcc
    37981 ggataaggcg tttacgccgc atccggcatt tgcttaacgt agagtgaaga ttaacctatc
    38041 tgcccattcc ccaaatgttt aacgcggcgc ttaacttctt cctgcttacc ggcgttaaat
    38101 ggacgtgcat ccgggctacc taaatatccg cacacgcggc gagtcaccga cacacgggag
    38161 gcgtcatggt taccacattt cgggcaagtg aagcctttgc tggtgcactc gaactcaccg
    38221 gtaaagccac actcgtagca ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc
    38281 tgatagctgt aatcccagac atcttccagc gccttcaggt tgtgctgaat gtttggatac
    38341 tcgccgtagc aaatgaaacc accgttcgct agcggcgggt aaggcgcttc aaagtcgatc
    38401 ttgtcgtacg ggttcacctt cttctccaca tcgaggtgga aactgttggt gtagtaacct
    38461 ttatcggtca cgcccggcac cacgccaaac tcagcagtat cgagacggca gaagcggtcg
    38521 cacaggtttt cactcggcgt gctgtagaga ctgaaaccat aacccgtttc ttctttccac
    38581 tgatccactg cctggcgcag acgttcaaca atggcgatac ctttcgcgcg aagctgctcg
    38641 ttgtcgtaaa catgctcgcc gccgaacagc gcgttaatgg tttcgtggat gccgatgtaa
    38701 cccagcgaaa tagacgcacg accgtttttg aagatttcag aaacatcatc gtcagcattc
    38761 agacgtacgc cacaagcacc ttccatatag aggatcgggg cgacgcgcgc tttcacgcct
    38821 tcgagacgag cgatacgggt catcagtgcc ttacgtgcca gcaccagacg ttcatccagc
    38881 agcttccaga aggtggcttc atcgcctttt gcttccagag caatacgcgg caggttcagg
    38941 ctgatcacgc cgaggttgtt acgaccatcg tggatctgct cgccgttttc attttcccac
    39001 acgccgagga agctgcggca gcccatcggg gttttaaacg aaccggtgac tttcactacc
    39061 tgatcgtagt tcaggatatc cggatacatg cgcttgcttg cgcactccag cgccagctgt
    39121 ttgatgtcgt agttcggatc gcctttttta tggttcaggc catcgcgaat cgcaaacacc
    39181 agtttcggga acaccgcagt tttacggttt ttaccaaggc ctgcgatacg gttacgcagg
    39241 atagattcct gaatcaggcg cgactcccag ctggtgccca gaccaaaacc aaaggttaca
    39301 aacggcgtct gaccgttggc ggtgtgcagg gtgtttactt cgtactccag cgactggaag
    39361 gcatcgtagc actctttaat ggtacgagag ttagcgtagc cttcggcgtc cgggatgctc
    39421 cactcttctg cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg tgccagcact
    39481 tcatcgatac ggttaatggt ggtgccgcca taaatatggc tggcaacctg agcaataatc
    39541 tgcgcagtta ccgcggttgc cgttgagatc gacttcggcg gttcaatctc ggcgttcccc
    39601 attttaaagc cctgggtcag catgcctttc aggtcgatca acatgcagtt gaacatcggg
    39661 aaaaacggtg agtaatcgag atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc
    39721 acgtcacgcg gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc ggccagcagg
    39781 tcgcgctggg ttggaatcac cttgctgtct ttgttggcgt tttcgttgag taacgaggcg
    39841 ttggtctgct cgaccagacc acggatctcc tggttcaggc gaccgcgttt ttcacgttca
    39901 atgtcgcgat cgtgacggta ctcgatgtaa gcgcgagcca gttgtttgta tggacccgac
    39961 atcagctggt tttcaactgc ggtctggatc tcgttgatat ccacctggtt gcggccctgc
    40021 atctgctcgc tgacaaccgc ggcaacagtg gcgcaataat cggcatcatc gacttccgct
    40081 gctttagctg cacgcagaat cgcttctttg atgcgctctg atttaaacgg cactttgcag
    40141 ccgtctcgtt tcatcacatg cggtgtcatg atcgctccat atttttaaga acaggttatc
    40201 cacagaaatt gggaaaggcg ttttccggtt ttttcgtttc gttttccggt gctttccgca
    40261 atcgccatcc gcttatccac aacgccgcac ctttttcgtg gggagctgta gtagcaatta
    40321 tagtcgatta atacaacata ttgggttggg acgcatttta aagtctatat atagtgcttt
    40381 gcatcaagga tgttttaact ttttttgatg tagctcaaag taaaaagcag agcgtacgga
    40441 tgacgggcgc tacagcgtta tgtaaatttt ttaatgaatt tgctggttga aaaatcaaca
    40501 aaaacaacat gctgacagac aaaaccccgg aatgaccggc attaccgggg cttagggaag
    40561 atttacttct gtaaccacca gacagcctca aaaggccgta aattcatggc acagggttgt
    40621 ggtgaggctt cttcgtagtt atgcatcaca agctgccagt tgccgcgcat ttgccctggc
    40681 tgccagggtt ggatctcacg gctaaggttg gcaatgacca gcaaggtttg ccccttccat
    40741 tcacggcgat agcaccacaa tacagggctg tttggcagca gatcctggta attgccccat
    40801 gtcaggatgg cttcttgctt acgcagtgcg attaactttt ggtaggtgta aaacaccgag
    40861 gactcatcgg ccagcgcggc ttctacgttg atttgttgat agttatcgcc caggccaatc
    40921 cacggttcgc cagccgtaaa cccggcatta tcgccgttgc tccattgcat gggcgtgcgg
    40981 ctgttgtcac gggatttact ggcgaggatt gccaataact cgtcggcatc acgaccatcg
    41041 ttgcgcagct cggcaaacat attgaggctc tccacgtcgc gatagtcagt aatgcgcgtg
    41101 aaatgcgggt tggtcatgcc aatctcttcg ccctggtaga tatacggcgt tccctgcatg
    41161 ccatgcagca ccatcgccag catttttgcc gcaggcacgc ggtattcacc ttcatcacca
    41221 aagcgagaaa caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt ccatgccacg
    41281 ttgtgcattc cttgttgcca gtggcggaac aatgttttca acgccacaaa gtcaggttta
    41341 gccagcgtcc atttttcacc accgggataa tcgaccttca ggtgatgaaa attaaaggtc
    41401 atcgacaatt cactgcctgt cagtgccgcg tatcgctggc aatgctcaag gctggtggag
    41461 gacatttcac ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc
    41521 aaaaactcgt gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc atccaggtct
    41581 tcagggaaac gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc
    41641 ccacgatcgg cccagaattc acagactttt ttcagctctg cgcgtaccgc tggattctcc
    41701 cagttgagat ccgcctgttc tggtgcaaag agatgcaaat agtactgttc gctttccgca
    41761 tgccagcgcc acgcactacc gccaaatttt gaacgccagt tgttcggtgg cgtttctggt
    41821 tcgccatcgc gccagatata aaactggcgg taagggcttt ctttgttcag cgcctcgcga
    41881 aaccaggcat gttgggtaga ggtatggtta aacaccatat cgagaatgat acgaatcccg
    41941 cgcgattttg cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca
    42001 atcgccgtat agttcgctac atcgtaaccg ttatcgacct ggggagagac ataaaagggg
    42061 gttagccaga tggcatcaac gcccagttta tgcagatagt ccaggcgttg gataacgcca
    42121 cgtaaatcgc cggtgccgct acccgtggtg tcctgaaaac tctttggata aatctggtag
    42181 ataacgccgt tttgccacca gtggggaaga ttagtcatta cgttattcct gcaaatgcga
    42241 gggggcgcaa ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca ggcggtattt
    42301 ccgctgatag ataaacgagg tgagtacaat cgggatgatg atggcgatag ccattgccag
    42361 cgcaaatacc tgccagtagc tcggttgaat cgagagaatt cccggcaggc cgcctacgcc
    42421 gatgccattc gccataacgc cgttcaggcc gcatagcaat cctgccagac cagaaccaat
    42481 catcgcgcac agcatcggga agcgatattt caggttgatg ccgtacattg caggctcagt
    42541 gaccccaagc caggcggaga tagcggcagg cacggagatc tcgcgttcat tgtgcttgcg
    42601 gctggaaatg ataatgccta tcacggcgga gccctgagcg atattcgaca gcgcaatcag
    42661 cggccacact ggcgtgccac ccatgctttg aatcatctgc aaatcaatag caagggtggt
    42721 ctggtgtacg ccggtgatca ccagcggcgc gtacaggaag ccaaacaatg ccgcaccaat
    42781 cggagcaaag ttgccggtca tcaggtgacg taccgcaaag gcaacgccat cgccaatcat
    42841 gcgaccaaac ggaccaatca gcgcatgggc gaggaacacc gcgaggatca gcgaacagac
    42901 gggtaccacc accagataga ggtaatccgg cacgatgcgt ttaaggcgag tttcaataac
    42961 gcccagcgcc agcccggcta acagtgccgg gatcacctgc gcctgatagc ctactttggc
    43021 gatgctgaac atgccaaagt cccacacttc cggcagctgc tgcccgagca gataagcgtt
    43081 catcagctgg ggagaaacca gtgtcacgcc aagcacgata ccaaggatcg gcgtgccgcc
    43141 catttttttc accgctgacc agcaaatacc gaccggcagg tagaagaaga tcgcttcacc
    43201 gatcaaccac agaaaatcgt agatcgtttg cagggaaggg tacatttgcg ccagcgtctg
    43261 accgttgctc atgggcaaat cgccgatcac attgcgaaaa ccgaggatca aaccgccgct
    43321 aatcaacgcg ggcagcagcg ggaagaagat ctccgcgaaa tgagagatca actgctcatg
    43381 ccatttcata ttctgccggg cggctttttt tacctgctct ttatcaacct gcgcctgtcc
    43441 ggttgacgca atcagtgctt gatagtaatc acccacattg gtgccaatca ccacctgaaa
    43501 ttgcccggca ttggtgaaac agcctttcac catggggagt tgctcaattt ctttcggtct
    43561 ggcattggcc ggttggttga ggacaaagcg caggcgagta atacagtggc tcaccgtcgc
    43621 aatattgccg cgcccgccga ccagttcaat caaccgatcg atatccgttt ggtttatttt
    43681 gctcatcata aagccccatg gcagatgaca tttttggttg gctgcagaat atattgcgcg
    43741 gaaaaattta aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag
    43801 gaaacgatca ggacagggtg gcggggatga tgatttgttg cggttcgctg cgcccggtta
    43861 cctgtgcgat caattggcag gccgcctggc gtccagcttc ggcgtaaccg ggatctacgg
    43921 tgacgatctc cggatggagg aatttcatta acggtgtatt accgacgctc gccagttgca
    43981 aggtgtcgat gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc gtgtcggttg
    44041 cgcacagtaa cgcagtagtt tcaggcgtaa tcacttttgc cacgttctca tagccttgct
    44101 tcatagcaag ccctggcaga gcggcaacgg gatgcagttt atgcgctttg cagaacgcca
    44161 ggtaggcttc gtgacgtcgc ttaccggttg tcacgtcact atgcggcacg ccgagataac
    44221 tgatattacg atgcccctgg tcatacagcc gttgcatcag gatcttgatt gccccttcgt
    44281 cgtcataaca gaccgaagca aagccttttg cgtcacgcgc cagcagaacc agcgatgact
    44341 gccagtgggc taacatttct tctgttatgc cagtaaaacc gaacagcact acgccgtcga
    44401 tattacgccg tttcagcacc cccaaatgtt cggcaactaa ttgtggggaa aactgacttt
    44461 ccatcatgat tgggtcgtaa ccttgttcat agaacgctgg cagcatggtt tgaacggcga
    44521 gattttctga caacgaatcc agacgggtaa caatgatggc gaccacttta tcgctttgcc
    44581 cacgcatagc gcgcgcagag cgggaagggg aaaatccatg ctgattcatc actgcttcaa
    44641 cacgctcgcg ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg gaaactgtag
    44701 atttccccac gccgcttaag cgtgcgatgt ctttgatggt cagccgattt tgcatcctgt
    44761 tgtcctgtaa cgtgttgttt aattatttga gcctaacttt acccgtgcat tcagcaatgg
    44821 gtaaagtctg gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc atagatgcta
    44881 cgaatattat tggattctcc ttattatttg cggcgctttt ttcacttacc ggaggttata
    44941 tggaacctga tcccacgcct ctccctcgac ggagattaaa acttttccgg taagcccgtc
    45001 ttttcacggc gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg ctcagcttta
    45061 ttaccttcag gtaaggcttc gccacgcctg aagacatttc tgtactgttt cagacagtgc
    45121 ggagggactc cttatgttta aagaaatttt tacccggctc attcgccatt taccttcccg
    45181 tctggttcat cgcgatccat tgcctggcgc gcagcagaca gtgaatacgg cggtcccgcc
    45241 gtccttaagt gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat tatggaaaac
    45301 gttcgacacc catccggaag ggttaaatca ggcggaagtg gaatctgccc gcgaacaaca
    45361 tggtgaaaat aaattacccg cacaacaacc gtcgccgtgg tgggtacatt tatgggtctg
    45421 ctatcgcaac ccatttaata ttttactcac cattctcggc gctatttctt acgccacgga
    45481 agatttattt gccgcgggcg ttatcgcgct aatggtcgct atttctacgt tgctgaactt
    45541 tattcaggaa gcacgttcca ctaaagcggc agatgccctg aaagcgatgg tcagcaatac
    45601 tgcgacggtg ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg agatcccgat
    45661 cgaccagctg gtgcccggcg atattataaa actggcggcg ggggatatga tcccggcaga
    45721 tttacgtatc ttgcaggcgc gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc
    45781 tctgcccgta gaaaaagcag ctaccactcg ccagccggag cacagcaatc cgctggagtg
    45841 cgacaccctg tgttttatgg gcaccaccgt ggtgagcggc acggcacaag caatggtgat
    45901 tgctacaggt gccaatacct ggtttggtca actggcgggg cgtgttagtg agcaggaaag
    45961 cgagccgaat gcctttcagc aagggatcag ccgcgtcagt atgctgctga ttcgctttat
    46021 gctggtgatg gctccggtgg tgctgttaat caatggttac accaaaggcg actggtggga
    46081 agcggcactg tttgcgcttt cggtcgcggt aggcttaacg ccggaaatgt tgccgatgat
    46141 tgttacctcg acgctggcgc gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa
    46201 acatctggat gctattcaga actttggcgc aatggatatt ctgtgcactg ataaaactgg
    46261 caccctgaca caggacaaaa ttgtgctgga gaatcatacc gatatctccg gtaaaaccag
    46321 cgaacgcgtg ctgcatagcg cgtggttgaa cagtcattat cagaccggac ttaaaaacct
    46381 gctcgataca gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc tggccagtcg
    46441 ttggcaaaaa atcgatgaga ttccgtttga tttcgagcgt cgccggatgt cggtggtagt
    46501 ggcagaaaat accgagcacc atcagctggt ttgcaaaggt gcattgcagg aaatcctcaa
    46561 tgtgtgttcg caggtgcgtc acaatggcga gattgtgccg ctcgatgaca tcatgctgcg
    46621 taagattaag cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc
    46681 gacgaaatac ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat
    46741 cctcgaagga tatattgctt tccttgatcc gccgaaagag acaactgctc cggcactgaa
    46801 ggcattaaaa gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg agttagttgc
    46861 agcgaaagtg tgccatgaag tggggctgga cgcgggagag gtggtcattg gtagtgatat
    46921 tgaaacgcta tctgacgacg aactggcaaa tctcgcacag cgtaccacgc tgtttgctcg
    46981 tctgacgccg atgcataaag aacgcattgt gaccttactg aagcgcgaag ggcatgtggt
    47041 tggctttatg ggcgatggta ttaatgatgc gcccgcctta cgcgctgcgg atatcggcat
    47101 ttctgtggac ggcgcggtag atattgcccg tgaagcggct gatatcatcc tgctggaaaa
    47161 aagcctgatg gtgctggaag agggggttat tgaaggacgt cgcactttcg ccaacatgct
    47221 gaaatacatc aaaatgacgg cgagctccaa cttcggtaat gtgttcagcg tgctggtggc
    47281 gagtgctttc ttgcccttcc tgccgatgtt gccgttacac ttgcttattc agaacctgct
    47341 gtatgatgtg tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa
    47401 gccgcagcgt tggaacccgg cggatctggg gcgctttatg atcttcttcg gaccgatcag
    47461 ctcgatcttc gatattctga cgttttgcct gatgtggtgg gtattccatg ccaacacgcc
    47521 ggaaacgcaa acgctgttcc agtcgggatg gtttgtggtg ggcttactgt cgcaaacgct
    47581 gattgtgcat atgatccgca cccgccgtgt gccgtttatt cagagctgtg catcgtggcc
    47641 gttaatgatc atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct
    47701 ggccagttat ctgcaattac aggcgctgcc gttaagctat ttcccgtggc tggttgcgat
    47761 tctggcaggg tatatgacat taacccagtt ggtgaaaggg ttctatagcc gtcgttacgg
    47821 ctggcaataa agaataaact gggcacgata gccccgattc agattgcaga caaagtaagc
    47881 tttgttcatg ccggatgcgg cgtaaatgcc ttatccggcc aacaaattca ataaattgca
    47941 ggaatcacgt aggcctgata agcgtagcgc atcaggcgat tttgcgtttg tcatcagact
    48001 taatccgggc atgatagccc ggatttccat caagattagc gacgaacagc gatcgcttcg
    48061 atctcaatct tcacgtcttt cggcagacgg gcgacttcaa cgcaagaacg tgccgggaag
    48121 gtggcgttgt gttcggtgaa gaaggcttcg taggtagcat taacggtagc gaagtcgttc
    48181 agatctttca caaacacggt ggttttaacg atgtcgccca ctttcaggcc agcggcttcg
    48241 acgatcgctt ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc tgccggtact
    48301 tcacccgttt tcggatttac cgggatctga ccggaggtga tgatcatatt gcccagatca
    48361 acgccctgta cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc
    48421 atgatttctc ctttattaca gcggtaaaaa gtctggctca ttatagggag ccagactttt
    48481 attaccaacc gcaattaatt ggccagcacc acattatggg aaaactcttt ttcacagtat
    48541 ttgcatttga gcgcgatgtc attggcgcgt tttcgcacgg caaagctgga agaaaccggt
    48601 tcggcatggc tgatacagtt gctgttcggg cagaccagca cattgtcgat gcgctctggc
    48661 agactggggc gcgatttacc caccacttca tagttgtcga tacggttaac cgtggcttgc
    48721 ggcgcataca atgccagttg atctacctga tcttcactca aaaaggtatt ttcgattttg
    48781 atcagatctt tgcggcccat ctcgccagaa ggcaagttca gaccgatggt gatgcgctga
    48841 tccgtttcgg tcagcttgaa cagactcaac agcttaaaac cgatctgggc ggggatatgg
    48901 tcaattaccg tgccgcgttt aatagcttca acctgcaatt tattatcgtg tgtcatctct
    48961 atttcccctt acagtaccag atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg
    49021 aaaatcccgt tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc cacatcggtg
    49081 gcaatctcat caacacgcgg cagcggatgc agcactttca tattggcttt ggcgttgtgg
    49141 agatcgctgg cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga cgggtccaga
    49201 cgctcttttt gcacgcgggt catgtacagg atgtctactt ccgccatcac ttcttcaata
    49261 gagctgtgca gactccatgc gatccctttt tcatcgagca tatccagaat gtattgcggc
    49321 attgccagcg cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc
    49381 tgagtcaggg agtgaacggt gcggccatat ttcaggtcac caaccattgc gacgtggaga
    49441 ttgtccagac gcccctgggt ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga
    49501 tgttggttgg agccatcacc ggcattcagt accggtacat tgccggaaaa ctcggtggcc
    49561 aggcgcgccg caccttcctg cggatgacgc atcactatcg catcgacgta agtgctgata
    49621 accgaaatgg tatcggccag cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg
    49681 gagaagccca ccacgctggc acccaggcgg tgcatagatg tttcgaaaga gaggcgggta
    49741 cgggtagagg cttcgaagaa acagctggca atgactttgt gcttcaacag ctctggttgc
    49801 gggtttgctt tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc gcgactaagg
    49861 tcgtttatgg aaatgatatg tttctgatat agcggattag ccatctttta tctcctgacg
    49921 cctgggcaaa aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa
    49981 aacggcaggc cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac acactgaacc
    50041 atacatcctc ccggcaaatt gtccggcatt atactcatag ccagaagcgg tgcaagcatt
    50101 tgatgcaata ttttgtcagc gcaaacggtt tatttgaatt aaaagtcaaa atgtatgcat
    50161 ttttatttgc tttgattctg gaagagaact gtcgttcggt atcaataatt gcagacattt
    50221 cctgcttttt tctttttcac tttcacgcaa tcagatatgc attttattcc attcatccga
    50281 cttatacggc ggagtgtgaa aacgaacggc taacactatt gcttacagct cagggatgcg
    50341 cgctatcact ttaatttcaa aatcaaagcc tgccagccag gtaacgccca ccgccgtcca
    50401 gtttggataa ggaggggcgc taaatatttc atttttgacc gtcatgatgt cttcaaattg
    50461 tttttccgga tcggtatgaa agctcgtaac atcaatgata tcgtcaaaag tgcatcccgc
    50521 agctgccagg gtcgcatgca aattatcaaa tgccagtctg acttgttgct gaaaatcggg
    50581 ttctggtgtt ccgtcctctc gacttcctac ttgcccggaa acaaacagca aatcgccgga
    50641 acgaatagcc gccgaataac gatgttcagc atacagtgaa tgtcggccag cagggaaaac
    50701 agcggttctt tctaccattt ggttatcctc aagatttacg acatgaacag aagatttata
    50761 tttaccggga gccgctttta gggaacgacg tgagtaaaca aaacccagac atcatggata
    50821 atggctgggc ttaattgagc gtagtcggtt atgccccaaa cgcgccatca atggtatgca
    50881 tcgcgccggt aacaaaactg gcttctggcc ctgctaacca tgcgaccatt ccagcgacct
    50941 cttccggttg cccatgtctt ttgatagcca taaaactatg caacatatcg cgcattggcc
    51001 cgttggcggg attagcgtcg gtatctattg gccctggctg gacgacgttg atggtgatcc
    51061 cacgcggtcc aaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg
    51121 cggcataagc agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta
    51181 agatgcgccc gccttcgggc atctgccggg cggcttcaac agaggcatgg taaggggcat
    51241 gaatattgat tttgaaaagg cgatcaatat cgtcggcatt taattccagg gcatcgccaa
    51301 agacgccaat acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat
    51361 caataacagc gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcatgtg
    51421 ccaggcgttc agcggcatct ttcgaccccg cataggtgaa tcgtacattg gctccatcgg
    51481 tgacgaaacg acgtacgata gcggcaccta ttccacggct accaccgagg atgagaactg
    51541 tcttacctgt aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa
    51601 actgttttaa ctttctgtca acaagttttg taatggtcac taaaaaacaa tctcgcgttc
    51661 caggtcgtcc cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc
    51721 accaaaaagg tttcgatgct gtcagtgttg ctgaagttac tgattatctt ggtattaacc
    51781 ccccgagtct ctacgcggct tttggcagta aggctgggtt atttagccgt gtactcaatg
    51841 aatatgtcgg tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccggtag
    51901 gggagtgcct ggctgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct
    51961 gcgctggctg tatggttctt gaaggcattc atagtcatga tccacaagcg cgtgatattg
    52021 ccgttcaata ttatcacgcc gcagaaacga ccatttatga ctatatcgcc aggcggcatc
    52081 cacaaagcgc acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga
    52141 aggcacgaga ggggcactcc ctcgaacaac tctgtgcaac agctgcactg gcgggggaag
    52201 cgataaaaac tattctcaag gagtgatgct gaccttgatc cgaaaggcgg gaacgcgcct
    52261 gccgataagt tgtgataaga caataattca cgcgttaagg ctggcggaat tgatcatctt
    52321 ttcgtataac ggtggaaatg aaacattgtt ttaattaagg agtggaaaag atgatcatcg
    52381 gaaatattca taatcttcag ccatggctac cccaggagtt acgccaggcg attgagcata
    52441 tcaaagcaca cgttacggca gaaacgccaa agggtaagca cgatatcgaa ggcaatcgtc
    52501 tgttttatct tatctcggaa gatatgaccg agccgtacaa agctcgccgt gcggagtacc
    52561 atgcccgtta tctcgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca
    52621 cacaacctgc aggtacgccg gagaccgact ggttagtaga taaagacatc gcatttttgc
    52681 cggaagtcgt tgatgagaaa acagttatcc tgaatgaagg tgattttgtt gtgttttatc
    52741 cgggggaagt gcataaaccg ctgtgcgcag tgggtgcacc agcccaggtt cgcaaagcag
    52801 tagtgaagat gctgatggcg tgatgacttt tcgccgtaaa taactccagg tttacggcga
    52861 gtttgtgaaa agagcgattt ttgatatttt tttgtgagta aaatttgtaa tgcttagacg
    52921 ttcttattaa ctcaaggagt tcgtcatggc aaaaatatca gatttgaatt gttatcaaca
    52981 catggcatca gccgacaatg ttaaaccaaa aaatgaagtc ttaaatacct ggtgtgtggg
    53041 aatgaatgat tttgctcgta atgccgaggg gcaggatagc agaaggaata ttctatctcc
    53101 tggagcattt ttggagtttt tggcaaaaat atttaccctg ggttatgtgg attttagcaa
    53161 atgctccaac gaagcgggta gaaatatgat ggctcatatt gagtcctcat cttat
//
LOCUS       DACADA010000012        52302 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.012, whole genome shotgun sequence.
ACCESSION   DACADA010000012 DACADA010000000
VERSION     DACADA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.012"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(20..910)
                     /gene="rarD"
                     /locus_tag="ISB17_000715"
     CDS             complement(20..910)
                     /gene="rarD"
                     /locus_tag="ISB17_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAP1978691.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRISK"
     gene            complement(962..1429)
                     /locus_tag="ISB17_000716"
     CDS             complement(962..1429)
                     /locus_tag="ISB17_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAP1978692.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1594..2463
                     /gene="pldA"
                     /locus_tag="ISB17_000717"
     CDS             1594..2463
                     /gene="pldA"
                     /locus_tag="ISB17_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAP1978693.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2588..4417
                     /gene="recQ"
                     /locus_tag="ISB17_000718"
     CDS             2588..4417
                     /gene="recQ"
                     /locus_tag="ISB17_000718"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP1978694.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4482..5102
                     /gene="rhtC"
                     /locus_tag="ISB17_000719"
     CDS             4482..5102
                     /gene="rhtC"
                     /locus_tag="ISB17_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAP1978695.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5140..6000)
                     /locus_tag="ISB17_000720"
     CDS             complement(5140..6000)
                     /locus_tag="ISB17_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1978696.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6159..6779)
                     /gene="rhtB"
                     /locus_tag="ISB17_000721"
     CDS             complement(6159..6779)
                     /gene="rhtB"
                     /locus_tag="ISB17_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAP1978697.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6890..7912
                     /gene="pldB"
                     /locus_tag="ISB17_000722"
     CDS             6890..7912
                     /gene="pldB"
                     /locus_tag="ISB17_000722"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAP1978698.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7920..8720
                     /gene="yigL"
                     /locus_tag="ISB17_000723"
     CDS             7920..8720
                     /gene="yigL"
                     /locus_tag="ISB17_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAP1978699.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8796..9695
                     /gene="bioP"
                     /locus_tag="ISB17_000724"
     CDS             8796..9695
                     /gene="bioP"
                     /locus_tag="ISB17_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAP1978700.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9583..10536)
                     /gene="metR"
                     /locus_tag="ISB17_000725"
     CDS             complement(9583..10536)
                     /gene="metR"
                     /locus_tag="ISB17_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAP1978701.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10654..12915
                     /gene="metE"
                     /locus_tag="ISB17_000726"
     CDS             10654..12915
                     /gene="metE"
                     /locus_tag="ISB17_000726"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP1978702.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            13254..13811
                     /locus_tag="ISB17_000727"
     CDS             13254..13811
                     /locus_tag="ISB17_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP1978703.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            13853..15361
                     /locus_tag="ISB17_000728"
     CDS             13853..15361
                     /locus_tag="ISB17_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP1978704.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            15412..17409
                     /gene="tkt"
                     /locus_tag="ISB17_000729"
     CDS             15412..17409
                     /gene="tkt"
                     /locus_tag="ISB17_000729"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1978705.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(17439..18284)
                     /locus_tag="ISB17_000730"
     CDS             complement(17439..18284)
                     /locus_tag="ISB17_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1978706.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(18484..19389)
                     /locus_tag="ISB17_000731"
     CDS             complement(18484..19389)
                     /locus_tag="ISB17_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978707.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNQQLMTRFVFN"
     gene            complement(19467..20690)
                     /locus_tag="ISB17_000732"
     CDS             complement(19467..20690)
                     /locus_tag="ISB17_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP1978708.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(20716..21105)
                     /locus_tag="ISB17_000733"
     CDS             complement(20716..21105)
                     /locus_tag="ISB17_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HAP1978709.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(21122..22078)
                     /gene="arcC"
                     /locus_tag="ISB17_000734"
     CDS             complement(21122..22078)
                     /gene="arcC"
                     /locus_tag="ISB17_000734"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1978710.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(22071..23495)
                     /locus_tag="ISB17_000735"
     CDS             complement(22071..23495)
                     /locus_tag="ISB17_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1978711.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(23492..25051)
                     /gene="fdrA"
                     /locus_tag="ISB17_000736"
     CDS             complement(23492..25051)
                     /gene="fdrA"
                     /locus_tag="ISB17_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1978712.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(25146..26006)
                     /locus_tag="ISB17_000737"
     CDS             complement(25146..26006)
                     /locus_tag="ISB17_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1978713.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(26012..26680)
                     /locus_tag="ISB17_000738"
     CDS             complement(26012..26680)
                     /locus_tag="ISB17_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP1978714.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(26937..27746)
                     /locus_tag="ISB17_000739"
     CDS             complement(26937..27746)
                     /locus_tag="ISB17_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP1978715.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            28008..28772
                     /gene="udp"
                     /locus_tag="ISB17_000740"
     CDS             28008..28772
                     /gene="udp"
                     /locus_tag="ISB17_000740"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAP1978716.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(28807..30297)
                     /locus_tag="ISB17_000741"
     CDS             complement(28807..30297)
                     /locus_tag="ISB17_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HAP1978717.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(30310..30816)
                     /locus_tag="ISB17_000742"
     CDS             complement(30310..30816)
                     /locus_tag="ISB17_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HAP1978718.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(30833..31807)
                     /locus_tag="ISB17_000743"
     CDS             complement(30833..31807)
                     /locus_tag="ISB17_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HAP1978719.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(31832..32461)
                     /locus_tag="ISB17_000744"
     CDS             complement(31832..32461)
                     /locus_tag="ISB17_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP1978720.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(32451..33455)
                     /locus_tag="ISB17_000745"
     CDS             complement(32451..33455)
                     /locus_tag="ISB17_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAP1978721.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(33479..34246)
                     /locus_tag="ISB17_000746"
     CDS             complement(33479..34246)
                     /locus_tag="ISB17_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1978722.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            34463..35890
                     /gene="rmuC"
                     /locus_tag="ISB17_000747"
     CDS             34463..35890
                     /gene="rmuC"
                     /locus_tag="ISB17_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAP1978723.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQNLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            35985..36740
                     /gene="ubiE"
                     /locus_tag="ISB17_000748"
     CDS             35985..36740
                     /gene="ubiE"
                     /locus_tag="ISB17_000748"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAP1978724.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            36754..37359
                     /gene="ubiJ"
                     /locus_tag="ISB17_000749"
     CDS             36754..37359
                     /gene="ubiJ"
                     /locus_tag="ISB17_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAP1978725.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            37356..38996
                     /gene="ubiB"
                     /locus_tag="ISB17_000750"
     CDS             37356..38996
                     /gene="ubiB"
                     /locus_tag="ISB17_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAP1978726.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            39075..39344
                     /gene="tatA"
                     /locus_tag="ISB17_000751"
     CDS             39075..39344
                     /gene="tatA"
                     /locus_tag="ISB17_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAP1978727.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            39348..39863
                     /gene="tatB"
                     /locus_tag="ISB17_000752"
     CDS             39348..39863
                     /gene="tatB"
                     /locus_tag="ISB17_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAP1978728.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            39866..40642
                     /gene="tatC"
                     /locus_tag="ISB17_000753"
     CDS             39866..40642
                     /gene="tatC"
                     /locus_tag="ISB17_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAP1978729.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            40684..41466
                     /gene="tatD"
                     /locus_tag="ISB17_000754"
     CDS             40684..41466
                     /gene="tatD"
                     /locus_tag="ISB17_000754"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAP1978730.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(41463..41951)
                     /gene="rfaH"
                     /locus_tag="ISB17_000755"
     CDS             complement(41463..41951)
                     /gene="rfaH"
                     /locus_tag="ISB17_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAP1978731.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            42118..43611
                     /gene="ubiD"
                     /locus_tag="ISB17_000756"
     CDS             42118..43611
                     /gene="ubiD"
                     /locus_tag="ISB17_000756"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAP1978732.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            43657..44358
                     /gene="fre"
                     /locus_tag="ISB17_000757"
     CDS             43657..44358
                     /gene="fre"
                     /locus_tag="ISB17_000757"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAP1978733.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(44539..45702)
                     /gene="fadA"
                     /locus_tag="ISB17_000758"
     CDS             complement(44539..45702)
                     /gene="fadA"
                     /locus_tag="ISB17_000758"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAP1978734.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(45712..47901)
                     /gene="fadB"
                     /locus_tag="ISB17_000759"
     CDS             complement(45712..47901)
                     /gene="fadB"
                     /locus_tag="ISB17_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAP1978735.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            48091..49422
                     /gene="pepQ"
                     /locus_tag="ISB17_000760"
     CDS             48091..49422
                     /gene="pepQ"
                     /locus_tag="ISB17_000760"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAP1978736.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            49422..50036
                     /locus_tag="ISB17_000761"
     CDS             49422..50036
                     /locus_tag="ISB17_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAP1978737.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            50075..51526
                     /gene="trkH"
                     /locus_tag="ISB17_000762"
     CDS             50075..51526
                     /gene="trkH"
                     /locus_tag="ISB17_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAP1978738.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            51538..52083
                     /gene="hemG"
                     /locus_tag="ISB17_000763"
     CDS             51538..52083
                     /gene="hemG"
                     /locus_tag="ISB17_000763"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAP1978739.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        12446 a        13154 c        13928 g        12774 t
ORIGIN      
        1 ttcagaatat atttattttt tatttggaaa ttctacgctg cgtataaatc gcatccatca
       61 caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg gcacccggtt
      121 tttcaccata aaacgtcaca gccagcagga acatcagcgt cggaccaatg tactggaaaa
      181 agcctaacgt tgagagacgc aggcgtgtcg cagctgcagt aaaacacaac agtggcacgg
      241 tggtgacaat accggcggcg atcagcagta aattcagcga catcgggttt tgccccatat
      301 gactggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc agcaaccaca
      361 tagtttcgat taacatgccg gtttgcgctt caacagcaat cttcttgcgc accaggccgt
      421 agaaagcaaa actaaacgcc agccccagcg cgatgatcgg cagtgaacca aaagtccaca
      481 actgaaccaa cacgccacaa atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      541 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag tgggttaata aagtaaccaa
      601 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
      661 caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
      721 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
      781 tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagtttg aagtacgctg
      841 gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
      901 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
      961 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
     1021 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1081 aacgctctcc tctgcctggg cgcagataat cgacgcgaag atcaatggtc cccatccgcg
     1141 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccca
     1201 cgcacaccag acctgcggcg acatccagcg ccgacgcaat gaccccgccg tgtaaaatgc
     1261 tttgcgccca gttgcccacc atcatcggct gatttttaaa ggccagctgt gcgaactctt
     1321 tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga taaacaaaca
     1381 tctcacccac taatttgagg gcttgttcag cggtcagtac ggcagacata acatccttac
     1441 acttcattag ttaatgaaat gttgatttta tgcttattta ttgttggttt ctactttagg
     1501 aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa aaatattcac
     1561 cttatcaata attcgttacg gagaacacga gcgatgcgga ctctgcaggg ctggttgttg
     1621 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     1681 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     1741 ttcacccttt atccttatga caccaactac atcatctaca cccaaaccag cgatctgaat
     1801 aaagaagcga ttgccagtta cgactgggcg gaaaacgcgc gtaaggatga agtaaagttc
     1861 cagttgagcc tggcgtttcc gctgtggcgt gggattttgg ggccaaactc tgtactgggc
     1921 gcctcctaca cacagaaatc ctggtggcag ctttctaata gcgatgagtc atcaccgttt
     1981 cgtgaaacta actacgaacc gcaattgttc ctcggttttg ccacggatta caactttgct
     2041 ggctggacgc tgcgcgatgt ggaaatgggc tacaaccacg actcaaacgg gcgttcagac
     2101 ccgacttccc gcagctggaa ccgcctttat acccgcctga tggcagaaaa cggtaactgg
     2161 ttggtagagg tgaagccgtg gtatgtggtg gggaatacgg acgataaccc ggacatcacc
     2221 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtactgagc
     2281 gcgaaagggc agtacaactg gaacaccggt tacggcggcg cggagttagg cttaagctac
     2341 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggttacgg cgaatcgctc
     2401 atcgactata acttcaacca gacccgcgtt ggtgtgggag ttatgctaaa cgacctgttt
     2461 tgatgaatgg ttggcaaatc tggaatccag catccaggat taccctctca gagactaaaa
     2521 gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg
     2581 ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca
     2641 agaaaccttt ggctaccaac agtttcgccc cggccaggaa gagattatcg acaccgtact
     2701 ttccggtcgc gattgcctgg tcgtcatgcc cactggtggc ggaaaatccc tttgctatca
     2761 aatccctgcc ttattgctaa acggccttac cgtggttgtc tcaccgctga tttcgttgat
     2821 gaaagatcag gtagatcaac tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac
     2881 gcaaacccgc gagcagcaac ttgaagtgat gacaggctgt cgcaccgggc aaatcagcct
     2941 gctttatatc gcgccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg
     3001 gaatccggtg ttattagccg ttgatgaggc gcactgtatc tcccaatggg gtcacgattt
     3061 ccgcccggaa tacgctgccc tcggtcagct gcgtcagcgt tttccgacgc tgccgtttat
     3121 ggcgctgact gccacagccg atgacaccac gcgccaggat atcgtgcgcc tgctggggct
     3181 gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct acatgctgat
     3241 ggagaaattc aaaccgctcg atcagttgat gcgttatgta caggaacaac gcggtaagtc
     3301 ggggattatt tactgtaata gtcgtgcgaa agtagaagac accgctgcgc gcctgcaaag
     3361 caagggaatt agcgcggcgg cctatcatgc cgggctggaa aataatgttc gcgctgatgt
     3421 gcaggaaaaa ttccagcgcg atgacctgca aattgtggtg gcgacggtgg cgttcggcat
     3481 gggcatcaac aagcctaacg ttcgcttcgt ggtgcatttt gatattccgc gcaatatcga
     3541 atcctattat caggaaactg gacgcgccgg gcgtgatggc ctgcccgcgg aagcgatgct
     3601 gttttacgat ccggctgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg
     3661 gcagttgcag gatatcgaac gtcataaact caatgcgatg ggcgcgtttg ctgaagcgca
     3721 aacttgccgt cgtttggtat tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg
     3781 gaactgcgat atctgcctcg atccgccaaa acagtacgat ggttcaaccg atgcgcagat
     3841 tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg tggtggaagt
     3901 gattcgcggt gctaataatc agcgtatccg cgactatggt catgacaaac tgaaagtcta
     3961 tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc agctgattca
     4021 cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga cagaggctgc
     4081 gcgtccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct
     4141 caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa
     4201 attacgcaaa ctgcgtaaat cgattgccga tgaaagcaat gtcccgccgt acgtggtgtt
     4261 taacgacgct accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct
     4321 cagcgttaac ggcgttggta tgcgcaagct ggaacgcttt ggcaaaccgt ttatggcgct
     4381 gattcgtgcg catgtcgatg gtgacgacga agagtagtca gcagcataaa aaagtgccag
     4441 gatgaagact ccgtaaacgt ttccccccgc gagtctaatg tatgttgatg ttattcctca
     4501 ccgtcgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc ttttttgtat
     4561 ctcagaccgc tgttagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggcattacct
     4621 gcggcgtaat ggtttgggct gggattgcgt tgcttggcct acatttgatt atcgaaaaaa
     4681 tggcctggct gcatacgctg attatggtgg gcggtggcct gtatctctgc tggatgggtt
     4741 accagatgct acgtggtgca ctgaaaaaag aggttgtttc tgcccctgcg ccacaagtcg
     4801 aactggcgaa aagcgggcgc agttttctta agggcttact gaccaatctc gcgaatccta
     4861 aagcgattat ctactttggt tcggtgttct cgctgtttgt cggtgataat gtcggcacca
     4921 ccgaacgctg gggcattttc gcgttgatta ttgtcgaaac actggcgtgg tttaccgtcg
     4981 ttgccagcct gtttgctctg ccgcaaatgc gctgtggtta tcaacgtctg gcgaagtgga
     5041 ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcgtt
     5101 aagcgcttcc cccctgatac gctatcaggg ggaataacat caggattgca attgataaag
     5161 gtcttcctga gaaagcccgg tcatttccat aatcaactgc aaatcaaagc cacgctgtag
     5221 catggctttt gcgacattga gaagggcatc gcgccgaagt tcttccgcta aagtcatcag
     5281 ttcctctcct tgttgaggca ctcgttgtgc cagaccatgt actaatgttc gtacatcctg
     5341 tgcttcacct accagcgcca tgtagtttat caacatgcgt atttgctgtc cgctcatctg
     5401 ccccagaatc attaagcggg ataacttatc taacagcgtt gtcatatccc gttgacggat
     5461 atgcttctgt accagcgtaa aggcggccat gctgcggtgt tgcatgattt cgtcatcagg
     5521 aattagcgta atatcaacca gcggaaaagc atgaaaatag agatcgcgtg caatgtccgg
     5581 atccggaaaa ccatcgagcc agtttgttga ccatggatat gggctacgct ttccctggta
     5641 aaaaagaatg gggatcacca gaggcagata atcatgtccc gcttcaagat gccgctgcat
     5701 ggtggcaata gcatagcgaa gcatacggaa cgccatattt tcatcaggtg aactttgatg
     5761 ctcaatcaac acatagatat atccgcaccc tttcgtcgtc tctactgaat aaagtacgtc
     5821 gttgtaatac tggcgtaggt tttcctctac aaaactgctg gactcgagat ggagcgtatc
     5881 aagattgcag agtttaacta aagaaggcgg cagatgaatt tcaataaagt cacgggccgt
     5941 ttctggtcgg gataaaaaag ccttaaagat tgcgtcatgt ggtgtcgttg cttccgtcat
     6001 ttttcatcat ccgtgggtga aatatggcgg cattctggca ctaacaaaga gaagtaaata
     6061 gtaaataaat taacagatta cgcttattac gagatgcctc gagcgcggat gtaacaatct
     6121 ctcgaggcac attaatttac gtgagcttta ttccagtttt aagcatgcct cgccgatgct
     6181 aacagcgctc ccaccagcat aaataacgag ccgaaaatct tattcagcgc cttcatctgc
     6241 tttggtcctt taatccatag agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca
     6301 ataatatcga ccacaatagt ggtgacgccg agcacgatat actgcatcag ctgcggctgt
     6361 tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca caatactttt gggattggtg
     6421 agattgacaa aaactgcgcg ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat
     6481 ttaagatcaa ttgcgccagc ggcgcgccac tgctggatcc caagccaaat caagtaagct
     6541 gcgcctgccc acttcaacac ttcaaacgca atcactgagc gggaaaatag cgtccccaac
     6601 cccacgccaa ccagcacaat atgaatcgcc agtccggtct gaagcccagc aatagacgcc
     6661 accgcgccgc gataaccgtg gttgagcgag gtggtcatgg tgttgattgc gccagagcct
     6721 ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa accaccattc taaggtcatg
     6781 atgaactccc ggtgtgtcta tttttgtgcc acaatacgct actgtcgcag cgttgtgtca
     6841 ggcacgctta aaaaaacgat tttacgtggt ttaagaggca gattacccga tgtttcagca
     6901 gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg gaccgctgac
     6961 tgatttctgg cgtcagcgtg atgaagcaga gtttactggt gtggatgaca ttccggttcg
     7021 ctttgtccgt tttcgcgcac agcaccatga ccgggtggta gttatctgcc cggggcgtat
     7081 tgagagctac gtaaaatatg cggaactggc ctacgacctg ttccattcgg ggtttgatgt
     7141 tttaatcatc gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg atccacatct
     7201 cgggcatgtt aatcgcttta atgattatgt tgatgatctg gcggcattct ggcaacagga
     7261 ggttcagccc ggtccgtggc gtaaacgcta tatactggca cattcgatgg gcggtgcgat
     7321 ctccacatta tttctgcaac gccatccagg tgtatgtgac gccattgcgc taactgcgcc
     7381 aatgtttggg atcgtgattc gtatgccgtc atttatggca cggcagatcc tcaattgggc
     7441 cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt
     7501 gccgtttgct atcaacgtac tgacccacag cagacagcga tatcgacgta acttacgttt
     7561 ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag
     7621 tattctggct ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc caacgcttct
     7681 cttacaagct gaagaggaac gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact
     7741 acgcaccgct gcgggccatc ctgtcgaagg aggacggccg ttggtaatta aaggtgcgta
     7801 ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg ctatcgttga
     7861 ttttttcaac aggcataact cacccagcgg aaaccgctct acagaggttt aaatttctta
     7921 tgtaccaggt tgttgcgtct gatttagatg gcacgttact ttctcccgac catacgttat
     7981 ccccttacgc caaagaaact ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg
     8041 cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga taatctggag attaagtctt
     8101 acatgattac ctccaatggc gcgcgcgttc acgatctgga tggcaacctg atttttgctc
     8161 ataacctgga tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac aatccggaca
     8221 tcattactaa cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga
     8281 tgcgcttttt caaagaagcg gtgttcaatt atgcgctgta tgagccggga ttgctggagc
     8341 cggaaggcgt cagcaaagtg ttcttcacct gcgattccca tgaaaaactt ctgccactgg
     8401 agcaagcgat taacgctcgc tggggcgatc gcgtcaatgt cagtttctct accttaacct
     8461 gcctggaagt catggctggc ggcgtttcaa aaggccatgc cctggaagcg gtagcgaaga
     8521 aactgggtta cagcctgcaa gattgtattg cctttggcga tggaatgaac gatgccgaaa
     8581 tgctgtcgat ggcggggaaa ggctgcatta tgggcagcgc gcatcaacgg ctgaaagatc
     8641 tgcatccaga gctggaagtg attggtacta acgccgaaga cgcagtgccg cattatctgc
     8701 gtaagctcta tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg atacttcgct
     8761 acaatggata ctcgttaatc aaagagtttt ccattgtggc gctacttatc atcaccacga
     8821 ttctgtgggc cttctccttt agcttttatg gcgagtacct tgcggggcac gtcgatagct
     8881 attttgcggt gctggtgcgc gttggtctgg cggcacttgt ttttctgccg tttctgcgta
     8941 cccgtggcaa tagcctgaaa acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg
     9001 gtgtgatgta tatgctgagt ttccgcgcct atctctacct gacggtttct gaactgctgt
     9061 tgttcaccgt actgacgccg ctctacatca cgctgattta tgacatcatg agtaagcgcc
     9121 gtctgcgctg gggctatgct tttagcgcct tgttggcggt gattggtgcc gggattattc
     9181 gctatgatca ggtcaccgac catttctgga ctggcttgtt gctggtgcaa ctctccaata
     9241 tcacttttgc catcggcatg gtgggttaca aacgtctgat ggaaactcgc ccgatgccgc
     9301 agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct ggtggcagtg attgcatggt
     9361 tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc attctggtgt
     9421 ttcttggcgt ggtggcttcc gggattggct actttatgtg gaactacggc gcgacgcagg
     9481 tggacgccgg aacgctgggc attatgaata atatgcacgt tccggcaggg ctgctggtaa
     9541 acctggctat ctggcaccaa cagccgcact ggccaacgtt tattacaggc gcgctggtga
     9601 tcctggcctc actgtgggtg catcgtaagt gggtcgctcc gcgctcttca caaacggcag
     9661 atgatcgcag gcgtgattgc gcgctgaacg aataaacgct tctgtaatcg gctgacgctg
     9721 ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag ccttcgccca gggttttggt
     9781 gaccaccaga ccctggcgtt caaaactctc caccacccaa tgaggtagtg cggcaatacc
     9841 catccgtgcg gcaaccatct gaatcaacaa taaggtgtta tcgacgcttt tcagtgacgg
     9901 gctgacgcct gctggctgaa gaaaatgccg ccagacatcc agtcggctac gctgcaccgg
     9961 ataaatcaac agcgtttcgc tggcgagatc ttccggtgta atacgcgttt ttgccgccag
    10021 tggatggtca ggtgctaaca ccagacgtac ttcatagtcg aacatcggcg aataatgcag
    10081 gccactgcgc ggcagaatat cggacgtcat caccagatcc agctcgccct gttgcaacgc
    10141 aggctgcgga tcaaacgtta cgcccgattt aaaatccatc tccacctgcg gccagttctt
    10201 atggaaattt tctaatgcgg gcgtcagcca ctgaatacag ctatggcact ctatggcaat
    10261 gcgcaggcgc gtctgctgcg gttcattgca ggcttgcagt gcctggctaa tttgcggcag
    10321 cacctgattt gccagttgca acaggatttc tccctgcggt gtaaagcgaa gcggctggct
    10381 tttacgcaca aatagccgga agccaaggcg ttgttccaga tcgctaaact ggtgagacag
    10441 ggcggattgc gtttgatgca acgtcgccgc agcggctgcg agtgagccgc agttccgcaa
    10501 cgcttgtagc gttttcaggt gttttacttc gatcatgaaa gtccttcact tcggcatgaa
    10561 taatttgcgc ttgaggaata tacagtaacc gccaattatg gatgtgtaaa catctggacg
    10621 gctaaatact aaaaattcca taaggggcat ataatgacaa ttcttaatca caccctcggt
    10681 ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc aagagagtta ttgggcgggg
    10741 aactccacgc gtgaagaact gctggcggta gggcgtgaat tgcgtgctcg tcactgggat
    10801 caacaaaagc aagcgggtat cgacctgctg ccggtgggcg attttgcctg gtacgatcat
    10861 gtactgacaa ccagtctgct gctgggtaat gttccgcaac gtcatcagaa caacgatggt
    10921 tcggtagata tcgataccct gttccgtatt ggtcgtggac gtgcaccgac tggcgaacct
    10981 gcggcggcag cggaaatgac caaatggttt aacaccaact atcactacat ggtgccggag
    11041 ttcgttaaag gccaacagtt caaactgacc tggacgcagc tgctggagga agtggacgag
    11101 gcgctggcgc tgggccacaa ggtgaaacct gtgctgctgg ggccggttac ctacctgtgg
    11161 ctgggtaaag tgaaaggtga acagtttgat cgcctgagcc tgctgaacga cattctgccg
    11221 gtttatcagc aagtgctggc agaactagcg aaacgcggca ttgagtgggt acagatcgac
    11281 gaacccgcgc tagtgctgga actgccgcag gcgtggctgg atgcatacaa acccgcttac
    11341 gacgcgctcc agggccaggt gaaactgctg ctgaccacct attttgaagg cgtaacgcca
    11401 aacctcgaca cgattactgc gctgcctgtt cagggtctgc atgtcgatct tgtacatggt
    11461 aaagatgacg ttgctgaact gcacaagcgc ctgccttctg actggctgct gtctgcgggt
    11521 cttatcaatg gtcgtaacgt ctggcgcgcc gatcttaccg agaaatatgc gcaaattaag
    11581 gacattgtcg gcaaacgtga tttgtgggtg gcatcttcct gctcactgct gcacagcccc
    11641 atcgacctga gcgtggaaac gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc
    11701 ctgcaaaaat gtcatgaact ggcattgctg cgcgatgcgc tgaacagtgg tgatacggca
    11761 gctctggcag agtggagcgc tccgattcag gcgcgtcgtc actctactcg tgtacataat
    11821 ccggcagtag aaaagcgtct ggcggcgatc accgcccagg acagtcagcg tgcgaatgtc
    11881 tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac tgcccgcgtg gccgaccacc
    11941 acgattggtt ccttcccgca aaccacggag attcgtaccc tgcgtctgga ttttaaaaag
    12001 ggtaatatcg acgccaataa ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt
    12061 gttgagcagg aacgtttggg actggatgtg ctggtacatg gcgaggccga gcgtaatgac
    12121 atggtggaat actttggcga gcatctggat ggctttgtct ttacgcaaaa cggttgggta
    12181 cagagctacg gttcccgctg cgtgaagcca ccgattgtta ttggtgacgt tagccgcccg
    12241 gcaccgatta ccgtggagtg ggcaaaatat gcgcaatccc tgactgataa accggtgaaa
    12301 gggatgttga ctggcccggt gactattctc tgctggtcgt tcccgcgtga agatgtcagc
    12361 cgtgaaacca tcgccaaaca gattgcgctg gcactgcgtg atgaagtggc cgatctggaa
    12421 gccgctggaa ttggcatcat tcagattgac gaaccggcat tgcgcgaagg tttaccactg
    12481 cgtcgcagcg actgggatgc ctatctccag tggggcgtgg aggctttccg tatcaacgcc
    12541 gccgtggcga aagatgacac acaaatccac actcacatgt gttactgcga gttcaacgac
    12601 attatggatt cgattgcggc gttggacgca gacgtcatca ccatcgaaac ctcgcgttcc
    12661 gacatggagt tgctggagtc gtttgaagag tttgattatc caaatgaaat cggtcctggc
    12721 gtctatgaca ttcactcgcc aaacgtaccg agcgtggaat ggattgaagc cttgctgaag
    12781 aaagcggcaa aacgcattcc ggcagagcgt ctgtgggtca acccggactg tggcctgaaa
    12841 acgcgcggct ggccagaaac ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat
    12901 ttgcgtcggg ggtaaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag
    12961 cgacgtcttc ccagcatact gcgtaaacca ttccagatat cagttcatac cctgtttaag
    13021 aaaatcttct ttattccctt ctcatgaaat aaatttcgtc atttccgcca ttttttctat
    13081 ctgcctctcc atcacatatt aaaaattact tgatatctct atgtttttta atgaaaaaca
    13141 tatcaaccta tgatctttct tgtgactagc ttcacatttc atactgatgc attggaatga
    13201 aataaaattt catgagattt tgtgtttgag aaaattattt catgaggtgt gaaatggacg
    13261 caaagctgta catcactgag atcatcaatg aattaacctt cgttaaggac tctatccgtg
    13321 aggatgactg caccaaattg atcgacacta ttcagcaaag ccagcgcgtt ttctgctatg
    13381 gcctgggacg cgccggtttt tctatgaaag ccttcaccat gcgcctgatg catatgggaa
    13441 aagaggttta cttcctgacg gaaaccatta cgccgaactt tgggcctggc gatctgttta
    13501 tcgtttcttc tgcttccggt gagacagcac aactggtggc gctggcgaaa aaagcacgtc
    13561 agctcggcgg cgctgtggcg gtgttaacca cgaatcgaca cgccactatc accgagtttg
    13621 tcgacgtcat cgtgcaaatc aatgcccctt ccaaaaatca aaaagattct gttttccgct
    13681 cggcacagcc gatggcgtcg ttgtatgagc aagctttgct ggtgattgcc gatgcattag
    13741 tgatgaagat ggctgctgaa agtggtgcgc cggaaagtga actgtttaag cgccacgcca
    13801 atctggaata gtcgtcgccg ttatttgctc gataccctac aggagaataa aaatgagcaa
    13861 aaaaataaca tccttgttta acttcaagaa gttgcagaaa ttttcttctg ctctgatgtt
    13921 accaatcgcc attcttccgg cggcgggttt gattttagcc atcgggattt catttaacat
    13981 tgagctgctg gcgaattgcg gcggcctgat tttcgccaac cttcctttgc tgttttgtgt
    14041 cggtattgtg attggcctga caggtgatgg ttcttcatca ctttcagcga ttatcagtta
    14101 catgatcatg aatgccacga tgggaacgca acttggcatt actgcggagt ccgttgcagc
    14161 cagtaacggt gattatgcga tgatcctcgg ggtaccgacg ttacaaacgg gagtgtttgg
    14221 cggtctgatt gtcgccatct ttgtttatct gctctatacc cgcttccaca atattgaatt
    14281 gcctcagttt cttggtttct tcggtggaaa acgctttgta ccgattatta cggctgtggc
    14341 ggcaattatt gtcggctatt tattgccgtg gctgtggata ccggtacaaa aaggtctggc
    14401 gtcgctttca acgctggtga ccagtggaca taacgcgaac ttcgcggcct ttatttacgc
    14461 cgttggcgaa cgttcgctga ttccttttgg cctgcatcat atctggaacg tgccgtttta
    14521 ttacaacttt ggcgactaca tgaccaaatc cgggcaactg gtgacgggcg atattccggt
    14581 gttctttgcc caattgcgcg acggtgctcc gttgacggcg gggctgttta tgacaggtcg
    14641 tttcccgatc atgatgtttg cgttacctgc cgccgcgttg gcgatgtatc aggaagcaaa
    14701 accggaacgt aaggcgctga ttaaaggcct tttactctcc ggtgcgctga ccacatttgt
    14761 taccgggatt acggagccgc tggaatatgc atttttgttt gcagcgccgg ttctctacat
    14821 cattcacatc ttcttatatg ccacttcatt tgtgctgatg aacatcctga atgtgcacat
    14881 tggtcatcct tttgctggcg gtcttattga tttcgtactg aatggcgtta tgccaaaccg
    14941 cacgccgtgg tatctggtgt tcctggcagg cgcgggctat gcagcgattt actacaccgt
    15001 atttcgggta ctgatccgca agttgaatct gaaaacaccg gggcgtgagg atgaagagat
    15061 tgccgttgca accacattaa cgcgcgaaga acgtccatac caaatcatcg aagcagtagg
    15121 tggtttcgac aatatcgaag atgtggatgc ctgcgcaaca cgcctaaggc tggcgctggt
    15181 agacgataag aaagtgaatg aaaaacggct gaaggagttg ggggctgcgg gtttagtgaa
    15241 actgggtgat ggcggtgtac aggtgatatt tggcggcaaa tcgcaaatcc tgcgcgacga
    15301 aatcaaaacc gtaatgagca gaccgcgccc gacagaaatg acagtggcct gtgctaacta
    15361 attcgagatt agcgggctgg tttcagcccg ccagacagag agagctgaag tatgaattca
    15421 caattactgg ctaatgccat ccgaatgtta agtgttgatg cgatccagaa agcgaactcc
    15481 ggtcatccgg gcgcgccgat ggggatggcg gatattgccg aagtggtctg gcgtcgtcac
    15541 ctgcgtcata acccaaaaaa tccgcagtgg tttaaccgcg accgctatgt tcagtcgaac
    15601 ggtcacggct ccatgttgat ttacgctttg ctgcatctta caggctacga cctgtcgatg
    15661 gatgatattc gcgactttcg tcagctccac tcccgcacgc cgggtcatcc agaatatggt
    15721 tatacgccgg gtgttgaaac caccaccggc ccgttggggc agggcgtagc taatgcggtg
    15781 gggatggcga ttgcggagaa ggcgctggcg gcagaattta acaaaccggg ctttaatatt
    15841 gttgatcatc acacatggtt atttttgggt gatggctgtc tgatggaagg gatctcccac
    15901 gaggcctgtg gtcttgcagg aacgctgaag ctcggcaatc tgattgctat ctgggacgat
    15961 aacggtattt cgatcgatgg tcacgtagaa ggctggttcg ctgaagatac ggctgcccgt
    16021 tttcgtgcct acggctggca tgtcattgaa ggcgttgatg gtcacgatcc agaagcggta
    16081 gatgctgcgg tgcgtgaagc gaaaagcgtc acggataagc ccagtctgct gtgctgcaaa
    16141 acgattatcg gttttggttc gccaaataaa gccaacagcc acgattgcca cggctcggcg
    16201 ctgggtgcag atgaagtggc gctggtgcgt gaacgtctgc aatggccgta tgcccctttc
    16261 gaaattcctg gtgaaattta tgccgaatgg gatgcgaccg agaaaggcgc gcaggttcaa
    16321 caggaatggg atgcactgtt tgccgactac gcgaagcagt ggccggaact ggcggcggag
    16381 tttactcgtc gcatgaaggg tgatttgcct gctggctggg tcgagaatat gcagaaatat
    16441 gtgcacgatc tgcaatccca tccggcggcg ctggcaaccc gtcaggttag ccagaaatgc
    16501 ctcaatcact tcgcagatat gctgccggaa ctgatgggcg gctcggcgga cttgtcgccc
    16561 tctaacctga ctcgccatca gaaatcggtt gatttcaccg gagaaaatcc ggcggggaat
    16621 tacatctcct atggcgtgcg tgaatttggt atgtcggcca tcatgaacgg tcttgcgctg
    16681 catggtgggt ttatccctta cggcggtact ttcctgatgt ttatggaata tgcgcgtaat
    16741 gcactgcgca tggcggcgct gatgaagatc cgctcggtgt ttgtttatac ccacgatacc
    16801 atcggcctgg gcgaagacgg cccgacacat cagcccgttg agcaactggc gtcgctgcgt
    16861 ctgacgccga atatggaaac ctggcgcggt tgcgatcagg tggaagtggc ggtggcctgg
    16921 caacaggcga ttgagcgcaa agatggccca acgtcgttgg tattaacgcg ccaacctctg
    16981 gcgcaacagc cgcgcaccgc tgcacagttg gcagagattg cgcgtggtgg ttatgtactg
    17041 agtgattgtg acggtcagcc tgagatgatc ctcatttctg cgggttcaga gattgagtta
    17101 gtggtcagcg ccgcaaaagc gttaacggaa gaagggcgta aagttcgcgt ggtttccatg
    17161 ccgtgtaccg agcgtttcga taatcaggat gcggcgtaca aagagtcggt attgccaaaa
    17221 gctgtacgca aacgtctggc ggtagaagcc agcatcgccg gattctggga acgctacgtg
    17281 ggcctggacg gtaaagtgat cggcatgacg agctttggtg agtctgctcc ggcgaatgtg
    17341 ttgttcaaac actttggttt tacgccggaa aatgtcctgg cgcaggcgcg tgagctactg
    17401 aacagctaaa cctgcacaag gggtgaaagg atgacctttt accccttgaa acgggagcca
    17461 agaaactctt cacgggtgcg gtatttctcg taatgcgcct tgtttttata cataaacaac
    17521 agcaccagca tatcaataac gcccattgcc ggaacgcgag tggagtcgaa ggcttcaaag
    17581 aagattttat cgctgaccgc ggtattgagt tgatgggtgg agagttttga taacggagat
    17641 ttaatatagt tagtgataca aacggatttg gcaccgcgct ctttcaccac tttaaagata
    17701 tcgaccatta agttggtgct gccagagtta gaaataccaa tcgccaggtc gcgtttcccc
    17761 atagaatatg ccgcattaat ttgcatttcc gggctgacgt gaacaacggt ttttaatccg
    17821 agttggctga atttataagc actataaacg gccagtgggt ttgaatttcc ggcagcaaaa
    17881 aagaaaatag tatcgcaatt agcaagtagt ttgagaatgg actcaagcac gtcggcatta
    17941 aaaaattgcg ttgattgtat taaatcttcg gcctgttttt ttaataaaaa ggttgccaaa
    18001 gatgttatat cgctgaagtc ggtattttca ggatagctat tgtcaggatt aatgacttct
    18061 tttgctaatg caatttttaa atggtaataa ccctgataac ctaatttctt acaggtgcgt
    18121 acgatagtcg cttcactggc atggcttttt tctgatagtt cagtgacact atagttcacg
    18181 acttcatgtg cgtggctaag aatatagtca gcgacctttt tttccgcctg atgcagtgta
    18241 gggtaattgc tttgaatcag actttttacg gttatgttat tcataattag taaatttcat
    18301 tctgacagaa agtgctgatt ataacatagt tttcaggcgt ggataggtgt gaaaaaatga
    18361 aggtgtgcag tgaacgtaat gattaacaga ccggttaatt ttcatgaatt acgtcaactg
    18421 cacaaaaata atttaaacgg gcgctctaat taaagcaaga cttatatttt tatcattgta
    18481 ttgtcaatta aatacaaatc gtgtcattaa ttgctggttt cttaaatatt ctatgcacca
    18541 ctgacaggct ttgcccatac catcggaacg agcggcaaga aataacttgg tggcgtgttt
    18601 atgttcgcgc attggctttt taagcaattt accgctgttc aataacggta atgccagatg
    18661 atgtggaata taaccaatac cgacgttttg ttctattaag gcgattgccg tagcgaagtc
    18721 aggaacaaaa atgggttttt gtccttccaa caaccaggct tgcatcgggg ggaagttaac
    18781 ggaggtatcg cggatacaaa ccgctggata atgacgcaat tcactgtttt ctaatggatg
    18841 ccgcttcgct gccagtgggt ggagtgggct gacgacaaaa tcccattcca tttgtccgac
    18901 cggttcgcta atgatgcctt cactgcttgg tactgcatga ggagcaccgt agaccagatc
    18961 agcacgctta ctgtacagcg catcccagca gccgttgtat acctcaagat ctaccgtcag
    19021 tgttgttgat gtgaattgcc gttcaaaatc tcgaataaat gccaccagca aatcaccagg
    19081 aataatattg tttaccgcaa ttgtcagttc acgctcaata ccatcgtgaa tcagcactgt
    19141 gttgcgcttt aatgcttcca ggtcgccaag aatagttttg ctatgttcga tgaagtaact
    19201 tcccgctggc gataattcaa tatagcgtcc cttacgaatg aacagttcaa cgcccaggct
    19261 ctcttccgct ttcttgatgg tgtaactgat ggcggaaggg actttattta actgctcggc
    19321 tgcggtggta atgctctggt gtctggcgac cagatgaata acacgaagcg tttcgtctga
    19381 gatgaacata aggctccctg gtcagtggta aagtggctga atctctccag attcagccgt
    19441 aatcattgcc tgagtatacc caggaattag cggtgttgac ttgcccgact gagaatcgca
    19501 tacgcgatac atgccaccag aatcgagtct accgaaggga tcgtcgtcag ggtgaataag
    19561 ccttttacgg tcataaagcc aaccgctgcg cctatgatcc atgctgcgaa tgtcagcact
    19621 ttaatctgtg actcctgggc taacactgac tcttgatagc cattacggcg ataaaggaaa
    19681 aagtcagcga tataaattcc ggcaacgggt ggcgtggcga tacccaggaa cagcagaaac
    19741 ggaataaacc acgccataat atccatactg cctacgatgg cggaaaggat acccagcgcc
    19801 acggtaatct gccatttggg aaagcgggta agcagtgtgg aaaccaccag cgtgccctgg
    19861 aacatattgc ccgcgttacc ggtgacgcag gcgaaaatca acaacagcgc cgcaggcaga
    19921 actgcaccga ataccgccat cgcacccagt aaagatccct gaccactaat ggcgctcggc
    19981 gttgcacccg cccagtagag cagagggtag gcaaccagaa acgtcacgcc tgcggcaatc
    20041 agcgcatgtt tgcggttatg cacaaaacta ccgaaatcag gcagtgtggc aaccagcaca
    20101 ataatggtgc caacaacggt ggaaacggcg acacctgtat tcatctccac aatcgccgct
    20161 ggtactgcca cttcggtatg ggttgccaca tagaggatgt aacacagcac cagcgcgata
    20221 accggcacgg caacctgggc aatttttccc agaacggcaa agccaaatgc cgtagaggcg
    20281 acaaaaatga cacagccagc cgccactaac agtgccagtg gaacttccag tccatgttga
    20341 gccagtaagt catgtaccga atgaccaaac atattggcgg taacggcaat ccagccgaac
    20401 agacttatcg ccatcaaaat gttaatggcg atggcccctt tttcgccaaa cgaatatttc
    20461 acaatgccat acgttggcag gcgggctttt tctcccacgc taatggtgat ggctgaaagt
    20521 aaggtcagaa tgacgccgcc taacaatacg acgctaatta aactgccgct ggaaagttga
    20581 ttgccgaggc ttgaggatga caacagcaca ggtgtgacgg caatacccag caaaatcatg
    20641 gcaatacgca atcctgatgc cgtattcaga ttctcttctt ttttacgcat agtatgttcc
    20701 ctttataaat gcgcgttaca cagtatgaaa atgcccggtg tatttacgcc agctacccag
    20761 ttctgtaatt tcccgttggc cttcggtcag ccagttttct gccagtacgc ccagcacttc
    20821 gctaccatcg gcaagtttga ttttgccaat tgccagccca gcgggttcgc tcattaacag
    20881 agcggcaaaa ctggccagcg gtaattgcca aatttctacg tcgacgtgtg ttcctccttc
    20941 attcacgcga atcattcccg gatggcgatc attaatactc cacaaccggt aatgggcgtc
    21001 agttcggtct tcacggacaa aaataccgcc cgctttttgc atatttgggt tgagttccag
    21061 gccgcgcatc agcgttccgt taacggctaa aagcgttgta gtcattgtgt tctccagaga
    21121 attaaagggt tatccatgta ccgctctgat gcgccagggc tgcttttatg gtttgcggtg
    21181 aagtaatcag gccgctggcc tgtttgcctt gttgctggct ggcattgatg aaatcaacaa
    21241 tggcttccac tttgggttgc atactgccga caccaaactg accttcccgc aacagcgttt
    21301 gcgcttcggc aacgctgata gcgtcgagcc actgttgttg tggtgtacca aagttaatcg
    21361 ccactttttc tacaccggtg gggatcacca gcagatctgc gcctaactga ctggcgagca
    21421 gcgcggaggc cagatcttta tcgatcacgg cttctacacc tttcagttgt tgcccgtctc
    21481 gcaccaccgg aataccgccg ccgccgcagg caataaccag atatccctgg cgcaccaggt
    21541 gagcgatggt gtcgtgctca ataatttcca gtggtgcagg agagggaact gtacgccgcc
    21601 aaccacgccc ggcgtcctcc atcagcgtcc agccgagttg ttgttggcgt tgttgggccg
    21661 ttgcttcatc gagaaacgcg ccaatcggtt tactggggct ggcgaaagca tcgtcatgtg
    21721 ggctgactcg cgtttgtgtc accagggcaa ttaccggttt gtttatgcca cggcgagcga
    21781 gttcgttatg cagcgctttc tggaacatgt agccaattgc accttgtgta tcacccacgg
    21841 cgtaatcaag tggaaccgga gaaacttcgt tactggcgag ttcagagcgg cgcagaataa
    21901 agcccacctg cgggccatta ccgtgggtta gcaccaggtc ccatccggct tcaaccatat
    21961 caacgatatg ctgcacgctt tccatcactg caacatattg atcgggaata ctattgcgtt
    22021 gctcgtcctg aatgagcgca ttgccgccaa ccgcgacgac agccagtggc ttaaccatga
    22081 tggttctcct ttgcgacgat ctccgccagt gcacgtacag cggcgacaaa acaggccatc
    22141 ggcgcagtgg tgatcccggc accaatttgc ccgacccccg cttgtttgtg ggcgataccc
    22201 gtattgatga ccggtaaaat gccgcgatcg gtcactttac gcgcatcaat ccctgctgcg
    22261 gtgggggcaa aattgagggc tggaagtgta aatgcggggt taccgccgag ggtgatggct
    22321 tgcatccgac ggctgttatt ggtggcatcg gcaggggtgc ctcccacaaa tttgacgatc
    22381 gccggagacg acgccatcgc gaagccaccc acacctgcgg tttcagtaat tgcgctatca
    22441 cccaggtcag cagcagcgtc gtcaacgcca aatccgggga agaacagacc ttcaacggca
    22501 ttggcaggag cctggaacca gcgatcgcca gtaccggaaa tccgaatacc aaaattcacc
    22561 ccgttacgcg ccatcaccgt aaccattgaa ctgaacggta cattggcggc ggcatccatc
    22621 attgctttac aggcggccat cgacagattg aggaagaagt gatcgttacc ggtgataaat
    22681 gccacgacgc gctggatatg ttcctgtgga agagagcagg ccagcaatgc cgggagcagg
    22741 cgcttaatca gcaaaccggt agcggcagca ttgcggttat gaacttcatc ccccatatgc
    22801 agcgcctgcg ccattaatgg cttgagttcc agctcgccgt gttgtgcgat tgctgctttc
    22861 attgccggcg tcagttcatc gcgcatccag gccagacgat tgagaacttc atcgttattg
    22921 gcgccaaaac ggagaacctt acccagacct tcattgaaat tgctaaaagt acggtggccg
    22981 ttggttttgt tttctaccac ccataacggc atcgacgggc tgatgatgcc tgccattggg
    23041 ccaaccgcgt gataatgatg acaaggggca aggtcgattt cgcctgcgtt tatctggttt
    23101 tcagcgtctt gctggctggt ggcccaacct tcgtacagca tggcaccaat aatcgcccct
    23161 tttaccggac cacacatctc ttcccaggca atcggcgggc cagagtgcaa aatacgtttt
    23221 tgttcagcca ttgctggaat ggcctcttta gcaagcatga catccaccag cacgggctgt
    23281 gcttcgagat aacgtgccat ggcgatttgg ttggcatttt ctaccagggg atgacgtagc
    23341 aacgatgcca gatccaatcc agcgtcaata tcgccttgtg ctggtggctg ccagttgagt
    23401 gcaattgcgc ttcccccggc atgttgaata ttgtcagcaa aactgtgtag tcccgcgttc
    23461 acaaccttca aaggctgatt aaataaggta ttcatgcgtt tagccccttt ttctgcaact
    23521 gagtctgtgc aacggtgctg gcccatagcg ccgcctgtgc attgctgctg gcgatgatga
    23581 cccctgcatt ttgtagtgcg cttatctgct gactgcgtat ttgcggatca gcttccgttc
    23641 cgcaaacgtg cgcaatcagc aacggagcgt tattcatgtc gatgtgtgaa agttggtcca
    23701 gtaattcgct ggcaggcgtc gtggaagcgc cataaccaag taccaggtcg aataacacga
    23761 cggcagtgtg tgagtcgttg agttcgttga gcagacgttg attgcgcaga gtcgggtcga
    23821 tcattggatg cggtttgcca cgggtgaaat catcgtcgcc catatcgatc aacgtgtgat
    23881 cttcgctttg ccagatattt gccagagcac gattacccgc aactggcgta ttggaagctg
    23941 cgctgaatcc cttttgttgg cagataagtt gcgcttcgta gcagaaggtt cctccggcga
    24001 aaacaccgcg tatcgcctgg cgatgggctg gcaggcgctg gcaggcgttt tgtaacattg
    24061 tgagatcgtc gcagggtaca tcggtttcga cagccggaag tggctgagca ttcagtaacg
    24121 ccacagcgat gtcggcggcg ctggcaagtg tggtggcggc agtgatattg ggacgtgcca
    24181 gatcatgcgg attcgccccg aggaaattca ccaccacggg ttttccacaa gcctcggcgc
    24241 gctccagaat agtccgggca acgatcggtg aaggcggttt tgaaatcagc acaattacgc
    24301 gagtttcatc gtcttgcgcc agcgcatcta aagcgaagag catcgatatt ccaccaatct
    24361 ctttactgag atcatgtccg cctgttccca acgcctgaga aatacccgct ccgagctggt
    24421 cgattcggca agtcacttcc tgtagcccgg tgcctgacgc gccaataaca ccaattgctc
    24481 cgcgtttcac cacattagcg aagcctaacg gaatgccgtt aacaatggcg gttccacagt
    24541 caggtcccat tacgatgcgc tgacgttctc gggcaagcgt tttgatggat ttttcctgca
    24601 cgatactgac gttatcgctg aacatcatca cgttcattcc gaggtttaac gctttaattg
    24661 cttcggcggc ggcgtagtct cccggtacag aaatgagtgc caggttggct tcgggttcgg
    24721 cttccagtgc catttccagg ctgacctttt ccggtgtttt aatgccacta tccgtttcat
    24781 catcgggttt gctggtcaga cgttgctggg ccagtatcag tgcttcattg caaatatctt
    24841 cttcacccat gactgcaatc actaaatcat tgggactggc gttgatttcg ttacccagac
    24901 ccgcatcgcg caattgttcg agattattgg gcgttcccat gacgaccgag gcctgttgaa
    24961 taccgggcaa cttgctgatt tgcgcggaaa tctgcatcag agaaactgag tcctgataaa
    25021 gattactgaa aactttatat ttaactttca tgctgttacg cctgtttcat tccgcccgat
    25081 agcaaaattc gggcaaagca tccccgtgta gccccataaa cgggctacgg gagaaaaggt
    25141 tgaaatcaat tcatggtgtt cagggttcga aagccatgca gcattcctgc aaggcaatct
    25201 actcctgacg ttgcgccaaa ttgcatgacc tgttctgcac aggatgcgat tgtcattgcc
    25261 gatgcgctcc caaccagttg tgcgagtaac tggcaaatcg gttctgaaaa atgtccctga
    25321 agcgcacgct ccagataatg acgactgata tccgtggtgt tgtgcgcatg ttgatcaatt
    25381 gcctgctgca accggtattg atgatcggca agtggtgcag ggagttgcca taaccagagc
    25441 gctgccagat agcccaacaa atagtcatct ccatcgggcg ttaagccttt accaaagccg
    25501 atcagttgtg gcacctggcg ttccagcgca tctggatgct ccataatcgc taatccctgg
    25561 tgcgtgagac tatccggcag gtgcaatgtg ctttgaacgt tatgctccag acaaaataaa
    25621 agccgttgcg atgccatcgt tgggtagtgc tggaaaacca aagggaatgc ctcaagtgtc
    25681 agcattcccc ccacaaatgg cgactgccag agcgttgcgt tatcgagcgc cacacaaaat
    25741 cgaggtgtcc gcagcaagcc tggggttaac tgaatgggat ctgaatgggc aatctctctt
    25801 cgccagtccc aacattcagg tagccagact ctggcagcat cagggagatt cagatacgtt
    25861 tgcgcacaca acagagtgat gaatgttccc gcgtctgaat acagatttac cgcatgttta
    25921 aaagcgctat gtagacgtaa atcttgcgtt tcatcctgcg ggaaaagata tcccaacgac
    25981 gatgcgcaaa gcgtaacctg taccatcggc gttagccttc cagacctgca ataatggctt
    26041 tggaatcagt aacccaaccg aaaatggcac cttgtgcttt gatcatctcg agggcgtatt
    26101 tctggaattc cgggaaatag gagccgacac aatcttccgg gataatgcat tcatagccac
    26161 gatcatttgc ttcgcgcacg gtggtggtta cgcaaacttc ggttgttaca ccacaaacta
    26221 tcagcgtctt gataccgtgg ttttgcaaaa tcagatgtaa gtcggtttga taaaacgcgc
    26281 ctttgccagg cttatcaatc accggttcgc ctgccaccgg gtacagttca ggaatgatgt
    26341 catgacctgc ttcacctctg accagaatgc gtcccatcgg tcctggttca ccaataaagg
    26401 tttttccgcc gcgcgtcagt ttggctggcg ggcagtcgct taagtctgca cgatggcctt
    26461 cacgagtatg aataaccata attccttttt gacgtgcggc tgccagcacc tctgtacatg
    26521 gtgcgatagc cgtgcgtacc cgtgaaacat cattgcccag tgcctcaccg aagcccccgg
    26581 cttcgacaaa gtcacgctgc atgtcgatca tgaccagtgc agttgtgcgc ggatcaaaag
    26641 gtaattcaaa gggttgagcc tgaaaaatgg attgcgtcat ggtaaatctc ctgtctgatg
    26701 tttaaacgat ccagttgttc tgagaacgag tattcaggag aaaagcggca caaaacagga
    26761 aaaaagtttg ccgcacagca acgaaatttt tgcattcagc aggtggtgtg atgacgaatg
    26821 tgaagggagt caaagagtgg catacagcaa caaatttttt gatgctgtat ggttttgaga
    26881 ttaaaaaaat cgttgaaatt gtgtgagctt aatcatcaga tttagcgact atggcgttac
    26941 tttttaacac catactgtgt aaaccattcc aacattcgct gccagccgtc tttcgcagac
    27001 tcggcatgat agctcggacg ataatcggcg ttgaacgcat gctctgcatc cgggtacacg
    27061 ataatctctg ctttcgcgtt tgctgcccgc agcgcctggc gcatcgtttc cacactctct
    27121 tgcggaatgc tgttatcctg gccgccatat aagccgagaa ccggcgcgtt aagatcggtt
    27181 gcgatatcaa caggttgttt cggtgaattc agcgacttat cgcccgtcag tttgccgtac
    27241 cacgccactg cggcttttag ctgtggatta tgcgcggcat acagccaggt gatacgtcca
    27301 ccccagcaga atccggtgat cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa
    27361 ctggcgacat gatcgagatc ggccagcacc tgcgagtcag gcacttttgc taccagaccg
    27421 ccaagcaacg tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca
    27481 ggtgcgatag ccagataccc ctccagcgcc agacggcgac aaatgtcgcg gatatgttca
    27541 tgcacgccaa aaatttcctg cactacaatg accactggca gtgggccatc gctttgcttt
    27601 ggtctggcat ggtaagcagg catgttatcc ccttgtgaag ggatagaggt gaagcccgcc
    27661 acaatcgcgt cgtccggagt ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca
    27721 aatccagatt gttgtgttgt tgccatggta ttctccgtac ccttataaaa atgttgcgca
    27781 atgttaacta tagtcagcat gcaacaaatc acattgcctg aatcggctca tcttttatgc
    27841 agtcctgcag aatgaagagt gatttatgtg atttgcatca cttttgatgg gtaaatttat
    27901 gcaacacatt ttcgtcatgg tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag
    27961 tgtctttttg cttcttctga ctaaaccgat tcacagagga gttgtatatg tccaagtctg
    28021 atgtttttca tctcggcctc actaaaaacg atttacaagg ggctacgctt gccatcgtcc
    28081 ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg gttaagctgg
    28141 catctcaccg cgaattcacc acctggcgtg cagagctgga tggtaaacct gttatcgtct
    28201 gctctaccgg tatcggcggc ccgtctacct ctattgctgt tgaagagctg gcacagctgg
    28261 gcattcgcac cttcctgcgt atcggtacaa cgggcgctat tcagccgcat attaatgtgg
    28321 gtgatgtcct ggttaccacg gcgtctgttc gtctggatgg cgcgagcctg cacttcgcac
    28381 cgctggaatt cccggctgtc gctgatttcg aatgtacgac tgcgctggtt gaagctgcga
    28441 aatccattgg cgcgacgact cacgttggcg tgacagcttc ttctgatacc ttctacccag
    28501 gtcaggaacg ttacgatact tactctggtc gcgtagttcg tcactttaaa ggttctatgg
    28561 aagagtggca ggcgatgggc gtaatgaact atgaaatgga atctgcaacc ctgctgacca
    28621 tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg ggttatcgtt aaccgcaccc
    28681 agcaagagat cccgaatgct gagacgatga aacaaaccga aagccatgcg gtgaaaatcg
    28741 tggtggaagc ggcgcgtcgt ctgctgaaat aaaaaaacgc tccccaactt cggggagcga
    28801 tatcagttat tgacgccgta aacgcattat tttgcggatt attggcgtca gtagcgtaaa
    28861 ggctgccaga gtcagcaatg ccagagtgat cggtcgggta tacagaaagt cgagtgaccc
    28921 accagaaagc gacagcgcac ggcgaaaatt actttccgcc attggcccca taatcaatgc
    28981 cagcactacg ggtgatgccg gatatcctcc tttctgcatg aaatagccca ccacgcctgc
    29041 aatcagcata atgccaacat caaaaaggct attgttcaac gcataagtcc cgaccacaca
    29101 aagtgcgagg atcatcggtg tgagaatatt gccgggaatt ttcaccacat taccgatgac
    29161 tttcaaagag agcagtccaa gaactagtag catgaagtaa cagaagatca tgccgacaaa
    29221 cagtgtataa accatatcgc cgtggtcggt aaacatcagc gggcctggtt gcattccctg
    29281 taacgttaac gcgcccagca tgatggcggt taccgcatcg ccagggatcc ccagcgtcag
    29341 cataggtaac aatgcgccac cggtgcagcc attcgcgcct gcctcacagg aggccacagc
    29401 tttaatttca cccttaccaa agttttctgg tgtcttactg aaacgacgag tttcgttata
    29461 ggcaacaaaa gctgcaatat ccgcccccgc accggggatc atgccgataa aaacccctaa
    29521 tcctgatgag cgcaaaatgg ttaatgcgat gcggcgtagt gtttgccagg gcagtaacag
    29581 actgccgata gccaccttcg ccccctgacg aatttgctgg ttttgctcca tagagcggaa
    29641 tgcttctgaa gcagcaaata aaccaatcat caccgggata aatggcacat tcatcagctc
    29701 cacaaatcca cctgtaaacc gtggaaaacc gcccattgga tcaaggccta tcgttgcgat
    29761 cagcaatccg ccaaccccgg caatcagccc cttaatcagc gactcaccgg aaatactggc
    29821 aataatgctc agtccgaagg ttgccagggc gaaggactcc gaggcactaa atttcagcgc
    29881 ccaactggca agtaccggag agagaaacat cagcacgata atgctgagcg ttccgccact
    29941 aaaggaagag aacgtggcgg cactcaatgc cagccctgct tttccctgct tcgtcagctc
    30001 atatccgtca atggcggtgg ctgcggcggc aggcgtaccg ggaattttga gcaaaattgc
    30061 ggtgatagaa ccgccataga cgccgccaaa aaagacgccg caaatcatca acaagccaga
    30121 aacaggctcc atgccgtagg tgaaaggcag caaaatcgcc acacccatgg tcgctgtcag
    30181 accaggcaga gcgccaatca caatacccag catcacccca aaagtggcaa acagcagcgc
    30241 ctgaggatta ttgagtaatg tcgaaaaacc ctgagttaaa agttcacttt caaacattgc
    30301 tggcccctgt tattcgaaaa atgtgccgac aggcaatggc acctgtaacg cttcgacaaa
    30361 cagcaaccag aggattgccg ttaacagcag cgcaataccc ggtgtgagta cccatcgcct
    30421 gtagcccatc aacgtcataa tgacgatgag gaaaacagga gtagtaatga gataaccgca
    30481 gtagctcatc gccacaatat aaaacgcggt tgccgcaatt cctgttgccg ttttcctgaa
    30541 tgccggtttt tcctgtgcgt tggcgcaaga ggctggtttt gcctgcaaaa gatcgcgtat
    30601 caccagcaag acagcgagca caatcagggc acaggcatag aaggtaggga aggcacttgc
    30661 accgggatcc gtttcaatgg cagttgctgg aaaatcagcg gcattaatca tcaccgcgcc
    30721 agcaaccaca caaagtaaca gcgccaccac catcgttgat gaaaggcgct tgcggtgcgg
    30781 tacggcgagt tgagcaacag actgttttgt gttcatcaac cgtacctcag gtttattttt
    30841 tcaggcccag gcgagtcagc aactcgtcaa agtacttttc ctgttcgctg atttgggtct
    30901 ggaagctggc agcgtcaagc catgcatagt tgagattcaa cttttgtagt gcatcctgga
    30961 atgcaggctc ttcagccgtc tcttttgctg cctttgccag aacatccacc acatcctgcg
    31021 gcgttttttg cgacacaatc aggccgcgcc aggtgccgat ggagagatca acgcctttct
    31081 cttttaacgt cgggacgtca ggcatggttt tcattcgctc atccgccatc actaccagtg
    31141 tcttcagctt gccgcctttc acatggttga taacttctcc tgggcttacg gaaaccgctt
    31201 caatgtgccc gcctaacagg cctgtaatgg caggggctgc gccgtcatac gggacatgag
    31261 aaaactttgc gcccgttttg tcttccagtg cagctgccgc cagatgccag atagctccgg
    31321 tgcctgagtt accaatgcgt acttttccgg gattcgcttt ggcgtaagtc ataaattctt
    31381 catagctatt ccacggcgca tcggcacgga ctgtgatagc agccggatcc gcattcagac
    31441 gggcaatggg tttaaagtcg ctggttttaa aacgcaccat tccgaggctg ggaagggtgg
    31501 tcagttcaac cgtgcctaaa ccaattttgt aaccgccagg gcgggcagcg gcgatttcac
    31561 tcaggccgat agcaccgcct ccgccaggtt tattgataac cccgatgctg acgggtaaat
    31621 ggtttttggc ggcatcagca aaggcacggg caaccagatc cgtaccgcct ccggcagcgt
    31681 agggaacgac taactcgatc tgtttggttg gccaggaggc agcctgtaca ggcgcaatgc
    31741 tgagggcagc gcctgcaaga agtaccattg cagccatttt tttcggtaag gtaagatgtt
    31801 ttatcattgc ttttatatcc ttatttatca gttagttgct atttaaattg ttccatgcgt
    31861 tcataaacag tactgcattt tcatagactt gctctggcgt catgccgggg tgatatagcg
    31921 cagaaccgag gccaaagccg tttgcgccgt ggctcagata acgcgccatg gtttccggtg
    31981 tgataccgcc aaccggtaac atcggaacgg attgtggaat aaccgcacgc caggctttgg
    32041 taacttctgg cgtaatttgt tcagccggga acagcttgat ggcattagca ccatgttcaa
    32101 tggcagtaaa ggcttcggtg ggcgtggcga cgccaggcgc acaatacata cccagcgcac
    32161 gggcgcgacg gatcaccgca gtatcgcaat gcggcatcac gataatctgg cccccagcct
    32221 ctttaaccgc aaccacctgc tctaccgaaa gcacagttcc ggctccgacc aggcagtcct
    32281 taggcagggc gtcgcgcagt aaactgatac tttgcaacgc ctccggcgag ttcataggaa
    32341 tctcaatcag gcggaatccg gcggcgtaca gtttttcacc gatggcgaca atctcatccg
    32401 gtttcacgcc gcgtaaaatg gcgactaacc cggttttttg caaaagcgta ttaaatggca
    32461 tgggtacatt ccctggcgtt ggtggacagt aacccggcag cgtgggcgat tcgccataat
    32521 ccctgttcag tggcattagg aatggcgtgt atctgtctgt cgggaaaaac gtggctgaat
    32581 gcttgtgaat agcggctgtt cagatttgcg ctaccgatta aggcgatcgg tgttgtggcg
    32641 ggtaaatcac gtagcaaact ggatgccaga ccacataatt catggcctat cagtaatccg
    32701 gaaaggtaat ccccctgcga ggacgacgta agattactgg tcagcaggcg cgaacgggtc
    32761 gaaaacagcg tcgataataa tccggtctga tgatgctctt gtgctgctgt cagcccgtgg
    32821 gtgaaggcat cccagtgcag ttcatctcca ggatgcatgg tggcaccgag aatcgaatgg
    32881 cgggataaaa catcaaataa ctcgcccgtc ataaaggttc ggaaatcggt aatggtgttg
    32941 ttttcaacta ctgcccattt cgcatgtgtg ccgggtaagc ctatcagcac tggcataccc
    33001 tgatggatcg cattttgcag ggacggttcc atcgagatag caccgaagat ttgtgtctct
    33061 tcgccacgca tcacttcggg catttcacct tctttaatca cgccaggaat gatgtgcagc
    33121 attgtctgct gctggtggat aacaggcgtc aactgcttcg ccagaccctc gagagtgacc
    33181 gggcaaggta aataagctgc tggttgccac ccttgcgcac tgccgagcat gccgcaagcc
    33241 agtaccgggc atggggtttg tgccagccag tcgccgcaga cacgtaaaaa cgtatcgtgg
    33301 aaagcgtcct cacgtgttgc tgcctgggag ggtaatttca tgatccccca ggggaattca
    33361 cgtttttctt gtggatttgg tgattcgcca aaacgccatg cccgaagcga tgacgttccc
    33421 cagtccaggg caataaatga gggtgctccc ggcattagtc gtttccttta atcatggttt
    33481 atttatcttt gcggacgtgc ttaccccagc ccagttcagc ggatatctct tctgcacagg
    33541 atttgacgta aggtgccagt tcttccagac gcgccagcga catatacgtg gtggtactgg
    33601 cgacagaaat agccgccaca atttcatccc gggcgttgta aaccggcgct gcaacgcaac
    33661 ggatagtcgg ctcgttctct tccagatcaa aagcaaaacc actggcggca taacgacgca
    33721 tgttctgaat aaatgcgccc agttttgttt catcaccaca ggtcttaaac agcgtgcgcc
    33781 actcttcttc tgttcggttg aggatcaatg ccttgccgat gccggtgatt gccagtggca
    33841 tgcgatggcc tgggcgtgag cgcatttcca gcccgcgctg actgttgatc ttctccagat
    33901 acagcacttc gtcgccttca acgatcccca gatgcacggt gtccagggtg taacgggcaa
    33961 ggcgttgcaa aacgggctgg gcaatttcgt acagcgaggt gctttccagt gctctggcac
    34021 cgaattcgat cagtttggca cccagttggt agccctgcgt tgggacatgg cgcaggtagc
    34081 gttgttccac cagaacggtg actaaacgat gggttgtgct ccgggtcgtg ccggtgaact
    34141 cgccgatact ttttaggtca tggcagccgt tataaacagc attaagaacc gccagtccac
    34201 gtaataacgt ttgtgcgcct gcggtggttg aagaggtttc tgccatacgt ttctcctgat
    34261 gatattgctg aagtgttcca cataatgacg tggggaaaag gtgatatatc ccacaatata
    34321 aacttcatct catattatgg gataaaataa tgaaaattaa cgatcgtttt gcgtagcgcc
    34381 tcgcaggaaa cttctttcaa actggacgtt tgtacagcac aattctattt tgtgcgggta
    34441 agttgttgcg tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag tcattgcgtt
    34501 ggtgggtgtg gcaattggct ggctgtttgc cagttatcaa catgcgcagc aaaaagccga
    34561 gcaattagct gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac aacaaattac
    34621 ccaaagcgag cactggcgcg cagagtgtga attgctcaat aacgaagtgc gcagcctgca
    34681 aagtattaac acctcgctgg aggccgatct gcgtgaagtg accacgcgga tggaagccgc
    34741 acagcaacac gctgacgata aaattcgcca gatgatcaac agcgaacagc gcctcagtga
    34801 gcaatttgaa aaccttgcca accgtatttt tgaacacagt aatcgtcggg ttgatgagca
    34861 aaatcgccag agtctgaaca gtcttttgtc gccgttgcgt gaacaactgg acggttttcg
    34921 ccgtcaggtt caggacagtt tcggtaaaga agcacaagaa cgtcataccc tgactcacga
    34981 aatccgcaat ctccagcaac tcaacgcgca aatggcccag gaagcgatca acctgacgcg
    35041 tgcgctgaaa ggcgacaata aaacccaggg caactggggc gaagtggtgc taacccgcgt
    35101 actggaggcg tctggtctgc gtgaagggta tgaatatcag actcaagtga gtatcgaaac
    35161 cgacactcgt tcgcgaatgc aaccggatgt catcgtgcgg ctgccgcagg gcaaagatgt
    35221 ggtgatcgac gccaaaatga cgctggttgc ctatgaacgt tatttcaacg ctgaagatga
    35281 ctacacacgt gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt
    35341 gctgggacgc aaagattatc aacagctgcc ggggctgcga actctggatt acgtgctgat
    35401 gtttattccc gttgaacccg cttttttact ggcgcttgac cgtcagccgg agctgatcac
    35461 cgaagcgttg aaaaacaata tcatgctggt tagcccgact acgctgctgg tggcgctgcg
    35521 cactatcgcc aacctgtggc gttatgagca tcaaagccgc aacgcccagc aaatcgccga
    35581 tcgtgccagc aagctgtacg acaagatgcg tttgttcatc gatgacatgt ccgcgattgg
    35641 tcaaaatctc gacaaagcgc aggataacta tcgccaggca atgaaaaaac tctcttcagg
    35701 tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt ttaggggtag aaattaaacg
    35761 cgagattaat ccggatttgg ctgaacaggc ggtgagccag gatgaagagt atcgacttcg
    35821 gtcggttccg gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca
    35881 gtcgcgctag cccattgggg gtagttaagc cgggtagaaa tctagggcat cgacgcccaa
    35941 tctgttacac ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac
    36001 aagaaacgac gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg
    36061 cccacgtttt ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcattcg
    36121 gtattcatcg tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga
    36181 ccgtgctgga tctggctggt ggcaccggcg acctgacagc gaaattctct cgcctggtcg
    36241 gagaaactgg caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggccgcg
    36301 agaagctgcg taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg
    36361 cgctgccgtt cccggataac acctttgatt gcatcaccat ttcgtttggt ctgcgtaacg
    36421 tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc
    36481 tgctggtgct tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat
    36541 actccttcca tgtgctgccg cgtattggct cactggtcgc gaacgacgcc gacagctacc
    36601 gttatctggc agaatccatc cgtatgcatc ccgatcagga taccctgaaa accatgatgc
    36661 aggatgccgg attcgaaagt gttgactact acaatctgac ggcaggggtt gtggcgctgc
    36721 atcgtggtta taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca
    36781 ggaattgaaa gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacggcccgc
    36841 tcgcgtctgt tgggcaaagt gctgcgcgtg gaggtaaaag gcttttcgac gtcattgatt
    36901 ctggtgttca gcgaacgcca ggtcgatgta ctgggcgaat gggcaggcga tgctgactgc
    36961 accgttatcg cttacgccag cgtgttgccg aaacttcgcg atcgccagca gcttgccgca
    37021 ctgattcgta gtggtgagct ggaagtgcag ggcgatattc aggtggtgca aaacttcgtt
    37081 gcgctggcag atctggctga gttcgaccct gcggaactgc tggcccctta taccggtgat
    37141 atcgccgctg aaggtatcag caaagccctg cgcgggggcg caaagttcct gcatcacggc
    37201 attaagcgcc agcaacgtta tgtggcggaa gccattactg aagagtggcg tatggcaccc
    37261 ggtccgcttg aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat
    37321 gccctgacca aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc
    37381 tatatttcat cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc
    37441 gtatcaccct gccgctacgg ctatggcgat actcattatt ctggatgcca aatcggcata
    37501 aagacaaacc tttaggtgag cgactacgac tggccctgca agaactggga ccagtatgga
    37561 tcaagttcgg gcagatgtta tcaacccgcc gcgatctttt tccgccgcat attgccgatc
    37621 agctggcgtt attgcaggac aaagtcgctc cgtttgatgg caagctggcg aagcagcaga
    37681 ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc
    37741 cgctggcttc tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag
    37801 aggtggtgat taaagttatc cgcccggata ttttgccggt cattaaagcg gatctgaaac
    37861 ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctgcgcc
    37921 caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg
    37981 aatctgccaa cgccattcag cttcgacgca attttgaaga cagcccgatg ctctacatcc
    38041 cggaagttta ccctgactat tgtagtgaag ggatgatggt gatggagcgt atttacggca
    38101 ttccggtgtc tgatgttgcg acgctggaga aaaacggcac caacatgaaa ttgctggcgg
    38161 aacgcggcgt gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgctgata
    38221 tgcaccctgg caacatcttc gtaagctatg aacacccgga aaacccgaaa tatatcggca
    38281 ttgattgcgg gattgttggc tcgctaaaca aagaagataa acgctatctg gcggaaaact
    38341 ttatcgcctt ctttaatcgc gactatcgca aagtggcaga gctacacgtc gattctggtt
    38401 gggtgccacc agataccaac gttgaagagt tcgaatttgc cattcgtacg gtctgtgaac
    38461 ctatctttga gaaaccgctg gccgaaattt cgtttggaca tgtactgtta aatctgttta
    38521 atacggcgcg tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc
    38581 tgctctacgt cgaaggggta ggacgccagc tttatccgca actcgattta tggaaaacgg
    38641 cgaagccttt cctggagtcg tggattaaag atcaggtcgg tattcctgcg ctggtgagag
    38701 catttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaactggttt
    38761 acgacagttt gcgccagggc aagtatttac agcatagtgt tgataagatt gcccgcgagc
    38821 ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt
    38881 tagtattaag tggcacattc ttgttggtca gccgacctga atgggggctg atgcccggct
    38941 ggttaatggc aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt
    39001 ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc atcatctacc
    39061 acagaggaac atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg
    39121 ttgtactgct ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga
    39181 tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagccagg
    39241 atgctgattt tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg
    39301 ctaaaacaga agacgcgaag cgccacgata aagagcaggt gtaatctgtg tttgatatcg
    39361 gttttagcga actgctattg gtgttcatca tcggcctcgt cgttctgggg ccgcaacgac
    39421 tgcctgtggc ggtaaaaacg gtagcgggct ggattcgtgc gttgcgttca ctggcgacaa
    39481 cggtgcagaa cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa
    39541 aggttgaaaa ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaat
    39601 tacgccaggc tgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga
    39661 gcgatgaagc gcacaccatc cataacccgg tggtgaaaga caatgaaact gtgcatgaag
    39721 gcgtaacgcc tgctgctgca caaacgcagg ccagttcgcc ggaacagaag ccagaaacca
    39781 cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc
    39841 cttcgtcgag tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca
    39901 tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc tcggtgatcg tgatattcct
    39961 gtgtctggtc tatttcgcca atgacatcta tcacctggta tccgcgccat tgatcaagca
    40021 gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat
    40081 caagctgacc tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg
    40141 ggcgtttatc gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt
    40201 ttccagctct ctgctgtttt atatcggcat ggcgttcgcc tactttgtgg tctttccgct
    40261 ggcatttggc ttccttgcca ataccgcgcc ggaaggggta caggtatcca ccgacatcgc
    40321 gagctattta agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct ttgaagtgcc
    40381 ggtggcaatt gtgctgctgt gctggatggg gattacctcg ccagaagact tacgcaaaaa
    40441 acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc cgccggatgt
    40501 cttctcgcaa acgctgttgg cgatcccgat gtactgcctg tttgaaatcg gtgtcttctt
    40561 ctcacgcttt tacgttggta aagggcgaaa ccgggaagag gaaaacgacg ctgaagcaga
    40621 aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac
    40681 aggatgtttg atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat
    40741 gttgtagcgc gcgcttttga cgcgggagtt aatgggctac tcatcaccgg taccaatctg
    40801 cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg gtcaacggcg
    40861 ggcgtacatc ctcacgacag cagccagtgg caagctgtga ctgaagaagc gattattgag
    40921 ctggccgcgc agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgcaac
    40981 ttttcgacgc cggaagagca ggaacgcgct tttgttgccc agctacgcat tgccgcagaa
    41041 ttaaacatgc cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattgctg
    41101 gagccgtggc tggataaact gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa
    41161 gagatgcagg cgtgcgtggc gtgtggaatt tatatcggca ttaccggttg ggtttgcgat
    41221 gaacgacgcg ggctggagct gcgggaattg ttgccgttga ttccggcgga gaaattgctg
    41281 atcgaaactg atgcgccgta tctgctccct cgcgatctca cgccaaagcc atcatcccgg
    41341 cgcaacgcgc cagcccatct gccccatatt ttgcaacgta ttgcgcactg gcgtggagaa
    41401 gatgccgcat ggctggctgc caccacggat gccaatgtca aaacactgtt tgggattgcg
    41461 ttttagagtt tgcggaactc ggtgttcttt acgctgtgct taatttcttt attgataaga
    41521 ttaagcaata gcatggagcg agcctctccg tctggctcgg tgaaaatggc ctgaaaacct
    41581 tcgaatgcgc cttcagtaat aatcacttta tcaccaggat acggcgtcga tggatcgaca
    41641 atatctttcg gtttatacac tgataactga tgaatcaccg ccgatgggac tatcgctggc
    41701 gacgcgccaa agcgcacgaa gtggctgaca ccgcgggtcg cgttgatagt cgtggtatga
    41761 atcacttccg gatcgaactc cacaaacaag tagttgggga acaatggctc actgactgca
    41821 gtacgtttgc cacgcacgat tttttccagg gtgatcatcg gtgccaggca attcacagcc
    41881 tgtctttcga ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac
    41941 caggattgca taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac
    42001 gctaagttaa ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca
    42061 aatttacagc atcgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg
    42121 gacgccatga aatataacga tttacgcgac ttcttgacgc tgcttgaaca gcagggtgag
    42181 ctaaaacgta tcacgctccc ggtggatccg cacctggaaa tcactgaaat tgctgaccgc
    42241 actttgcgtg ccggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta ctcaatgccg
    42301 gtgctgtgca acctgttcgg tacgccaaag cgcgtggcga tgggcatggg gcaggaagat
    42361 gtttcggcgc tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc ggagccgcca
    42421 aaaggtttcc gcgacctgtt tgataaactg ccgcagttta agcaagtatt gaacatgccg
    42481 acaaagcgac tgcgtggtgc accctgccaa caaaaaatcg tctctggcga tgacgtcgat
    42541 ctcaatcgca ttcccattat gacctgctgg ccggaagatg ccgcgccgct gattacctgg
    42601 gggctcaccg taacgcgtgg cccgcataaa gagcggcaga atctgggcat ttatcgccag
    42661 cagctaattg gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg cggcgcgttg
    42721 gattatcagg agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc tgtggcgctg
    42781 ggtgccgatc ctgccacgat tctcggtgca gtcacccccg ttccggatac gctttcagag
    42841 tatgcgtttg ccggattgct gcgcggtacc aagaccgaag tggtgaagtg tatctccaat
    42901 gaccttgaag tgcccgccag tgcggagatt gtgctggaag ggtatatcga acaaggcgaa
    42961 actgcgccgg aagggccgta tggcgaccat accggttact ataacgaagt cgatagtttt
    43021 ccggtattta ccgtgacgca tattacccag cgtgaagatg cgatttatca ttccacctat
    43081 accgggcgtc cgccagatga acctgcggta ctgggtgtag cactgaacga agtgttcgtg
    43141 ccgattctgc aaaaacagtt cccggaaatt gtcgattttt atctgccgcc ggaaggctgt
    43201 tcttatcgtc tggcggtagt gacgatcaaa aaacagtacg ccggacacgc gaagcgcgtc
    43261 atgatgggcg tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc
    43321 gatgatgacg tcaacgcccg cgactggaac gatgtgattt gggcgattac cacccgtatg
    43381 gacccggcgc gggatactgt tctggtagaa aatacgccta ttgattatct ggattttgcc
    43441 tcgcctgtct ccgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggt
    43501 gaaacccagc gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt
    43561 gacgccattt gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt
    43621 gttttgccct atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg
    43681 acctcggtag aagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc
    43741 ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg
    43801 ttctcaatgg cttcgacgcc agatgaaaaa gggtttatcg agctgcatat tggcgcttct
    43861 gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg
    43921 gtcgatattc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg
    43981 attgcgggcg gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt
    44041 aacccaaacc gtgatatcac catttactgg ggcgggcgtg aagagcagca tctgtatgat
    44101 ctctgcgagc ttgaggcgct ttcgttgaag catcctggtc tacaagtggt gccggtggtt
    44161 gaacaaccgg aagcgggctg gcgtgggcgt actggcaccg tgttaacggc ggtattgcag
    44221 gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa
    44281 attgcccgtg acctgttttg cagcgagcgt aatgcgcggg aagatcgcct gtttggcgat
    44341 gcgtttgcat ttatctgaga tataaaaaac ccgcccctga caggcgggaa gaacggcaac
    44401 taaaacttta tacagtggca ttactctacg ttgcatccgg caaacagtgc ccgatgcaac
    44461 gctgccgcgt cttatcgggc ctacaaaagt tctgaaccgt gggccggata aggcgttcac
    44521 gccgcatccg gcaagtggtt aaacccgctc aaacaccgtt gcaatgccct gacccagacc
    44581 gatacacatc gtcgccagac caaactggac gtctttgtgt tccatcagat tcagcagcgt
    44641 ggtgctgata cgcgcaccgg aacaacccag cggatgaccc agcgcgatcg cgccaccgtt
    44701 gaggttgatc ttctcgtcaa tctgctccat tagcccgaga tctttaatac acggcaggat
    44761 ctgcgcggca aaggcttcgt tcatctcgaa tacgccgata tcgctggcag aaagccccgc
    44821 ttttttcagc gccagcttcg aggccggaac cggaccataa cccataatag atgggtcaca
    44881 acccacaacc gccatcgaac gcacgcgggc gcgcggctta agacctaatt cacgggcgcg
    44941 gctttcactc atcaccagca tggcagctgc gccatcggaa agcgcagaag atgtacccgc
    45001 cgttaccgta ccgctgacgg gatcaaacgc cggacgcagt gtggcgaggg cttccacggt
    45061 ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aatacgccgt cggcatcgtg
    45121 accaccggtc gggatgattt catttttaaa tgcgcctgac tgcgtggcgg cccacgcgcg
    45181 ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt gcatacgcgc
    45241 cagcatttct gccgttaagc ccatcatgcc cgccgctttg gcgacattac ggctcaggcc
    45301 ggggtgaaaa tcgacgccgt gactcattgg cacatggccc atatgctcca cgccgccaac
    45361 cagacatgcc tgcgcatcac cggtcatgat cattcgtgct gcgtcatgca gtgcctgcat
    45421 ggatgaacca cacaagcgat taacggtaac cgccgggaca gagtgcggca cttcagccaa
    45481 cagtgccgcg ttacgggcga tattaaaacc ctgctccagt gtttgctgca cacaacccca
    45541 gtaaatatcg tcaagggccg ccgcttccag tgccgggtta cgcgccagca ggctacgcat
    45601 taaatgagcg gagagatctt ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc
    45661 catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc cttaagccgt
    45721 tttcaggtcg ccaaccggac gggctggctc aaccggggga tagtacggtt cgttatgacg
    45781 tgctttatta cgcagacctt ccggtacttc atacagcggg ccgaggtgct gatattgctg
    45841 tgccatatcg aggtattttg cgctgccgag ggtgtccagc cagcggaacg cgccgccgtg
    45901 gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttctgccg gagtggcgat
    45961 aatgccctct tccagacagc ggaccacttc gttgaccatc gggatcatca tgcgggcgat
    46021 aatctcttct tcgctgaaat cgcgcttcgg ctggctcact tttgccagca ggtcgtcaac
    46081 gacgacatct tcttctttct tcggcttacc tttgctgtct tctttataac gccagaaacc
    46141 gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg catcgcgata
    46201 atctttctgc atccgctgcg ggaagcctgc tgccatgaca gcctgggcgt gatgcgcggt
    46261 gtcaatgccc acaacatcca gcagatatgc cggacccatc ggccagccaa actgtttttc
    46321 catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcaactggc tgaaaccggc
    46381 gaaatagggg aacagtacac ggttgacgaa gaagccgggg cagtcgttaa ccacaatcgg
    46441 cgttttgccc atcttgctcg cccaggcgac gactttcgcg atggtttcgt cggagctttt
    46501 ctcgccgcga ataatttcta ccaacggcat tcggtggacc gggttaaaga agtgcatccc
    46561 gcagaagttt tccggacgtt ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt
    46621 gttcgacgcc agtacggtat ccgggcgtac tttctgctcg gtttctgcca gcacggcttt
    46681 tttcactttc gggttttcaa caaccgcttc taccacaaca tccacgcggt caaacccggc
    46741 gtagtcgagc gttgggtgaa ttgtggagat cacgccagcc agtttcagac catcgatctt
    46801 gccgcgctca agctgcttgt tcagcagttt cgcggcttcg gtcatgccga gggttaacga
    46861 cttgtcgttg atatctttca tgacaaccgg cacgcctttc cacgcagact ggtaagcgat
    46921 gccgccgccc ataatgcctg cgcccagcac agcggcctgt ttcggggttt caacatcttt
    46981 ggtgagtttc ttcgctttac cttttacata ttgatcgtta aggaaaatgc cgaccagtgc
    47041 gcgggcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt ttaaggcttc
    47101 ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg gggccggata
    47161 atgtttcccc gccgtttgtg cgaccatccc tttagcgatg gtgaagctca tggtggcttc
    47221 aatcttgctc agttttagcg gttccagctt cggctgacgt ttcgctttcc agtcgaggtc
    47281 gccgttaatg gcctggcgta aaatcgccat cgccccttca atcagttttt ctgctttgac
    47341 tacaccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt taccggcggc
    47401 aatgatttcc agcgcactgt cagcgcccag catacgtggc atacgtacag aaccgccaaa
    47461 gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg gcgtcgccag
    47521 acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc cgttgacggc
    47581 agcaatggtc ggcaccggga gatcttccag gcgattaaac acgctattgg caaagtgcag
    47641 ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg tgatatcagc
    47701 accgacgata aaggctgctt tgttcgaacg cagcagcagc ccttttagat ctgattgctg
    47761 ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga gtttattaac
    47821 tgaacctggg gcatcaaata ccagttcggc aatgccatct tccagccagt caaggtacag
    47881 ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca
    47941 gatgcatcga agtgtgtatt ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc
    48001 gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa gatgcggaga
    48061 cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcact ctataaaaat
    48121 catatcgcta ccttacaaga acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg
    48181 ttacttattc actccggcga actgttcaac gtttttctcg acgatcatcc ctatccgttt
    48241 aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa ctgctggctg
    48301 ctggtggatg gcgtgaacaa gccgaaactg tggttttatc tgccggttga ttactggcac
    48361 aacgtcgaac cgctaccgac ctccttctgg actgaagatg tagaagtgat cgcgctgccg
    48421 aaagccgatg gcattggtag cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt
    48481 ccggtgccgg aacgtgcgct gcaactgggt attgaggcca gcaacatcaa cccgaaaggg
    48541 gtgatcgact acctgcatta ctaccgctcc ttcaaaaccg agtacgaact ggcctgtatg
    48601 cgtgaagcgc agaaaatggc ggtcaatggt catcgtgcgg cagaagaagc gttccgttct
    48661 ggcatgagcg agttcgatat caacattgcc tatctgaccg cgaccggtca tcgtgatacc
    48721 gacgtacctt atagcaacat tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc
    48781 aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc cggggccgaa
    48841 tataacggct atgcggctga tctgacccgt acctggtcgg caaaaagcga caacgactac
    48901 gcacagctgg tgaaagacgt aaatgatgag caactggcgc tgatcgccac catgaaagca
    48961 ggcgtcagct atgtggatta ccatatccag ttccatcagc gcatcgccaa actgctgcgt
    49021 aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga
    49081 ccgtttatgc cgcacggtat cggccatccg ctgggcctgc aggtgcatga cgtagccggt
    49141 tttatgcagg atgatagcgg tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc
    49201 tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat ctacttcatc
    49261 gaatcgctgc tggcaccgtg gcgtgaaggg cagttcagca aacacttcaa ctggcagaaa
    49321 attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt gattcacgaa
    49381 aacaacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc tggttaattc
    49441 ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag ccgcttcatt acgctgttgg
    49501 cgcataccga tggcgttgag gcggcaaaag cgtttgttga atcggtgcgg gcagaacacc
    49561 ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat tctcaacagc
    49621 tgggtttctc tgacgacggg gagccggcgg gaacggcagg taaaccgatg ctcgcccaac
    49681 taatgggcag cggcgtcggg gaaattaccg ctgtggtggt gcgctactac ggcggcatat
    49741 tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc
    49801 agctaacgac ccaacgcaag acaccgttaa ccgaatatac tttgcaatgt gaatatagtc
    49861 agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc aacagtgatt
    49921 atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct gaattttccg
    49981 caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa gaataatccc
    50041 cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca ttacccgaat
    50101 cgttggacta ctggttatct tattttcagg gaccatgatt atccctgggc tggtagcact
    50161 catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg ccctcgccat
    50221 tggctctatg ctttggtggc cgaaccgcaa agagaagggt gaactgaaat cccgtgaggg
    50281 gtttctgata gtggtgctgt tctggaccgt gctgggtagc gtcggtgcgc tcccttttat
    50341 cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt tctctggcct
    50401 gaccaccacg ggggccacta cgctggtggg gctggattcg ctccctcacg ccatcctctt
    50461 ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttgg cggttgcgat
    50521 actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc ccggcccgct
    50581 gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta
    50641 tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gctggaatgg atgcctttga
    50701 tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac atgatgccag
    50761 tatcggttat ttcgacagcc cgactattaa cactatcatt gctatcttcc tgctgatctc
    50821 tggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga aggtttattg
    50881 gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt tcactggtgg ttatttgtac
    50941 actcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta accaggcgtt
    51001 tttccaggtg gtgtcgatgg cgacaaccgc cgggtttaca actgacagca ttgcccgctg
    51061 gccgctcttt ttgccggtac tgcttttatg ttcagctttt atcggcggtt gcgccgggtc
    51121 aacgggcggt ggcctgaaag tgatccgcat cctgttgctg tttaagcagg ggaaccgtga
    51181 gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga atcgcgcact
    51241 gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat tggtgtttat
    51301 tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct ttgcctcggt
    51361 tgttgcaaca ttgaataacc tggggccagg gcttggcgtg gttgctgata actttaccag
    51421 tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt
    51481 ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga gtaatacgtg
    51541 aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc ctcctacctg
    51601 gcttcggaac tgaaagaact ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa
    51661 gaaccacaat gggaaaacta tgaccgtgtg gttattggtg cttctattcg ctatggtcac
    51721 taccactctg cgttccagga atttgtcaaa aagcatgcga cgcgactgaa ttcgatgccg
    51781 agcgcctttt attccgtaaa tctggtggcg cgtaaaccgg agaagcgtac tccacagacc
    51841 aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg ctgtgccgtc
    51901 attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat gatcaagctg
    51961 attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta taccgattgg
    52021 gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa
    52081 taagcataaa gaataaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    52141 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
    52201 ggtcagcggg gttctcctga gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt
    52261 agcgggaaag cgtattatgc acaccccgcg ccgctgagaa aa
//
LOCUS       DACADA010000013        50174 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.013, whole genome shotgun sequence.
ACCESSION   DACADA010000013 DACADA010000000
VERSION     DACADA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.013"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            194..1141
                     /gene="cyoA"
                     /locus_tag="ISB17_000764"
     CDS             194..1141
                     /gene="cyoA"
                     /locus_tag="ISB17_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAP1978740.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1163..3154
                     /gene="cyoB"
                     /locus_tag="ISB17_000765"
     CDS             1163..3154
                     /gene="cyoB"
                     /locus_tag="ISB17_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAP1978741.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3144..3758
                     /locus_tag="ISB17_000766"
     CDS             3144..3758
                     /locus_tag="ISB17_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAP1978742.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3758..4087
                     /locus_tag="ISB17_000767"
     CDS             3758..4087
                     /locus_tag="ISB17_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAP1978743.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4099..4989
                     /gene="cyoE"
                     /locus_tag="ISB17_000768"
     CDS             4099..4989
                     /gene="cyoE"
                     /locus_tag="ISB17_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAP1978744.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5138..6502
                     /locus_tag="ISB17_000769"
     CDS             5138..6502
                     /locus_tag="ISB17_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1978745.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6630..7121)
                     /gene="yajQ"
                     /locus_tag="ISB17_000770"
     CDS             complement(6630..7121)
                     /gene="yajQ"
                     /locus_tag="ISB17_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAP1978746.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7289..8200
                     /gene="panE"
                     /locus_tag="ISB17_000771"
     CDS             7289..8200
                     /gene="panE"
                     /locus_tag="ISB17_000771"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP1978747.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8163..8753
                     /gene="yajL"
                     /locus_tag="ISB17_000772"
     CDS             8163..8753
                     /gene="yajL"
                     /locus_tag="ISB17_000772"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAP1978748.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8807..10255)
                     /gene="thiI"
                     /locus_tag="ISB17_000773"
     CDS             complement(8807..10255)
                     /gene="thiI"
                     /locus_tag="ISB17_000773"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP1978749.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10461..10703
                     /gene="xseB"
                     /locus_tag="ISB17_000774"
     CDS             10461..10703
                     /gene="xseB"
                     /locus_tag="ISB17_000774"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP1978750.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10703..11602
                     /gene="ispA"
                     /locus_tag="ISB17_000775"
     CDS             10703..11602
                     /gene="ispA"
                     /locus_tag="ISB17_000775"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAP1978751.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            11627..13489
                     /gene="dxs"
                     /locus_tag="ISB17_000776"
     CDS             11627..13489
                     /gene="dxs"
                     /locus_tag="ISB17_000776"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP1978752.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13544..14518
                     /gene="yajO"
                     /locus_tag="ISB17_000777"
     CDS             13544..14518
                     /gene="yajO"
                     /locus_tag="ISB17_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAP1978753.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14572..15090)
                     /gene="pgpA"
                     /locus_tag="ISB17_000778"
     CDS             complement(14572..15090)
                     /gene="pgpA"
                     /locus_tag="ISB17_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP1978754.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(15068..16045)
                     /gene="thiL"
                     /locus_tag="ISB17_000779"
     CDS             complement(15068..16045)
                     /gene="thiL"
                     /locus_tag="ISB17_000779"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAP1978755.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16123..16542)
                     /gene="nusB"
                     /locus_tag="ISB17_000780"
     CDS             complement(16123..16542)
                     /gene="nusB"
                     /locus_tag="ISB17_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP1978756.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16562..17032)
                     /gene="ribE"
                     /locus_tag="ISB17_000781"
     CDS             complement(16562..17032)
                     /gene="ribE"
                     /locus_tag="ISB17_000781"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP1978757.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17121..18224)
                     /gene="ribD"
                     /locus_tag="ISB17_000782"
     CDS             complement(17121..18224)
                     /gene="ribD"
                     /locus_tag="ISB17_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAP1978758.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18228..18677)
                     /gene="nrdR"
                     /locus_tag="ISB17_000783"
     CDS             complement(18228..18677)
                     /gene="nrdR"
                     /locus_tag="ISB17_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAP1978759.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18828..19367
                     /locus_tag="ISB17_000784"
     CDS             18828..19367
                     /locus_tag="ISB17_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP1978760.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            19666..20550
                     /locus_tag="ISB17_000785"
     CDS             19666..20550
                     /locus_tag="ISB17_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAP1978761.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20588..20935)
                     /locus_tag="ISB17_000786"
     CDS             complement(20588..20935)
                     /locus_tag="ISB17_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAP1978762.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21065..22036)
                     /gene="secF"
                     /locus_tag="ISB17_000787"
     CDS             complement(21065..22036)
                     /gene="secF"
                     /locus_tag="ISB17_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAP1978763.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22047..23894)
                     /gene="secD"
                     /locus_tag="ISB17_000788"
     CDS             complement(22047..23894)
                     /gene="secD"
                     /locus_tag="ISB17_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAP1978764.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23922..24254)
                     /gene="yajC"
                     /locus_tag="ISB17_000789"
     CDS             complement(23922..24254)
                     /gene="yajC"
                     /locus_tag="ISB17_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP1978765.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24277..25404)
                     /gene="tgt"
                     /locus_tag="ISB17_000790"
     CDS             complement(24277..25404)
                     /gene="tgt"
                     /locus_tag="ISB17_000790"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP1978766.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25459..26529)
                     /gene="queA"
                     /locus_tag="ISB17_000791"
     CDS             complement(25459..26529)
                     /gene="queA"
                     /locus_tag="ISB17_000791"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP1978767.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26622..27203
                     /gene="acpH"
                     /locus_tag="ISB17_000792"
     CDS             26622..27203
                     /gene="acpH"
                     /locus_tag="ISB17_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAP1978768.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27208..29025)
                     /gene="malZ"
                     /locus_tag="ISB17_000793"
     CDS             complement(27208..29025)
                     /gene="malZ"
                     /locus_tag="ISB17_000793"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAP1978769.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29181..30554)
                     /gene="proY"
                     /locus_tag="ISB17_000794"
     CDS             complement(29181..30554)
                     /gene="proY"
                     /locus_tag="ISB17_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAP1978770.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30630..31949)
                     /gene="brnQ"
                     /locus_tag="ISB17_000795"
     CDS             complement(30630..31949)
                     /gene="brnQ"
                     /locus_tag="ISB17_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAP1978771.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32356..33651)
                     /gene="phoR"
                     /locus_tag="ISB17_000796"
     CDS             complement(32356..33651)
                     /gene="phoR"
                     /locus_tag="ISB17_000796"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAP1978772.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            complement(33709..34398)
                     /gene="phoB"
                     /locus_tag="ISB17_000797"
     CDS             complement(33709..34398)
                     /gene="phoB"
                     /locus_tag="ISB17_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAP1978773.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34588..35790
                     /gene="sbcD"
                     /locus_tag="ISB17_000798"
     CDS             34588..35790
                     /gene="sbcD"
                     /locus_tag="ISB17_000798"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAP1978774.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35787..38930
                     /gene="sbcC"
                     /locus_tag="ISB17_000799"
     CDS             35787..38930
                     /gene="sbcC"
                     /locus_tag="ISB17_000799"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAP1978775.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39056..40240
                     /gene="araJ"
                     /locus_tag="ISB17_000800"
     CDS             39056..40240
                     /gene="araJ"
                     /locus_tag="ISB17_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAP1978776.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(40509..41417)
                     /gene="mak"
                     /locus_tag="ISB17_000801"
     CDS             complement(40509..41417)
                     /gene="mak"
                     /locus_tag="ISB17_000801"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAP1978777.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            41542..42453
                     /gene="rdgC"
                     /locus_tag="ISB17_000802"
     CDS             41542..42453
                     /gene="rdgC"
                     /locus_tag="ISB17_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAP1978778.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42534..42614)
                     /locus_tag="ISB17_000803"
     CDS             complement(42534..42614)
                     /locus_tag="ISB17_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978779.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42611..42952)
                     /locus_tag="ISB17_000804"
                     /pseudo
     CDS             complement(42611..42952)
                     /locus_tag="ISB17_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43100..43384)
                     /gene="ppnP"
                     /locus_tag="ISB17_000805"
     CDS             complement(43100..43384)
                     /gene="ppnP"
                     /locus_tag="ISB17_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAP1978780.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43456..44133)
                     /locus_tag="ISB17_000806"
     CDS             complement(43456..44133)
                     /locus_tag="ISB17_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAP1978781.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44391..44582)
                     /gene="yaiA"
                     /locus_tag="ISB17_000807"
     CDS             complement(44391..44582)
                     /gene="yaiA"
                     /locus_tag="ISB17_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAP1978782.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44632..45156)
                     /gene="aroL"
                     /locus_tag="ISB17_000808"
     CDS             complement(44632..45156)
                     /gene="aroL"
                     /locus_tag="ISB17_000808"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAP1978783.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45339..45797)
                     /locus_tag="ISB17_000809"
     CDS             complement(45339..45797)
                     /locus_tag="ISB17_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP1978784.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45917..46726
                     /gene="proC"
                     /locus_tag="ISB17_000810"
     CDS             45917..46726
                     /gene="proC"
                     /locus_tag="ISB17_000810"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP1978785.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46743..47858)
                     /gene="adrA"
                     /locus_tag="ISB17_000811"
                     /gene_synonym="dgcC"
     CDS             complement(46743..47858)
                     /gene="adrA"
                     /locus_tag="ISB17_000811"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAP1978786.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47960..48280)
                     /gene="psiF"
                     /locus_tag="ISB17_000812"
     CDS             complement(47960..48280)
                     /gene="psiF"
                     /locus_tag="ISB17_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAP1978787.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48399..49814)
                     /gene="phoA"
                     /locus_tag="ISB17_000813"
     CDS             complement(48399..49814)
                     /gene="phoA"
                     /locus_tag="ISB17_000813"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAP1978788.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49915..50163)
                     /gene="iraP"
                     /locus_tag="ISB17_000814"
     CDS             complement(49915..50163)
                     /gene="iraP"
                     /locus_tag="ISB17_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAP1978789.1"
                     /translation="MAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQNDQQRL
                     IDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12210 a        13396 c        12675 g        11893 t
ORIGIN      
        1 tgcacttccc tttcgttata acgccctttt gcaacagctt cttaaaatca acttgatatg
       61 ttttgcaaca tatgtgacct gatagccaaa tccaagtaac aggaatttaa tcatgtttac
      121 agtaatgtaa ccttcccgta aaatgcccac acactttaaa cgccaccaga tcccgtggaa
      181 ttgaggtcgt taaatgagac tcaggaaata caataaaagt ttgggatggt tgtcattatt
      241 tgcaggcact gtattgctca gtggctgtaa ttctgcgctg ttagatccca aaggacagat
      301 tggtctggag caacgttcac tgatactgac ggcatttggc ctgatgttga ttgtcgttat
      361 tcccgcaatc ttgatggctg ttggtttcgc ctggaagtac cgtgcgagca ataaagatgc
      421 taagtacagc ccgaactggt cacactccaa taaagtggaa gctgtggtct ggacggtacc
      481 tatcttaatc atcatcttcc ttgcggtact gacctggaaa accactcacg ctcttgagcc
      541 tagcaagccg ctggcacacg acgagaagcc cattaccatc gaagtggttt ccatggactg
      601 gaaatggttc ttcatctacc cggaacaggg cattgcaacc gtgaatgaaa tcgctttccc
      661 ggcgaacact ccggtgtact tcaaagtgac ctccaactcc gtgatgaact ccttcttcat
      721 tccgcgtctg ggtagccaga tttatgccat ggccggtatg cagactcgcc tgcatctgat
      781 cgccaacgaa cccggtactt atgacggtat ctccgccagc tacagcggcc cgggcttctc
      841 aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc gcattcgacc aatgggtcgc
      901 aaaagcgaaa cagtcgccga acagcatgtc tgacatggca gcgttcgaaa aactggccgc
      961 gcctagcgaa tacaaccagg tggaatattt ctccaacgtg aaaccagact tgtttgccga
     1021 tgtgattaac aagtttatgg ctcacggtaa gagcatggac atgacccagc cagaaggtga
     1081 gcacagcgca catgaaggta tggaaggcat ggacatgagc cacgcggaat ccgcccatta
     1141 aaggggttga ggaagaataa agatgttcgg aaaattatca cttgatgcag tcccgttcca
     1201 tgaacctatc gtcatggtta cgatcgctgg cattattttg ggaggtctgg cgctcgttgg
     1261 cctgatcact tacttcggta agtggaccta cctgtggaaa gagtggctga cctccgttga
     1321 ccataaacgt ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc tgcgtggttt
     1381 tgctgacgcc attatgatgc gtagtcagca ggcccttgcc tctgcgggcg aagcgggctt
     1441 cctgccacct caccactacg atcagatctt caccgcacac ggcgtgatca tgatcttctt
     1501 cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc agatcggcgc
     1561 gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc tggtttaccg ttgttggtgt
     1621 gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct ggctggccta
     1681 tccaccgcta tcgggaatag agtacagtcc gggagtcggt gtcgattact ggatatggag
     1741 tctccagcta tccggtatag gtacgacgct taccggtatc aacttcttcg ttaccattct
     1801 gaagatgcgc gcaccgggca tgaccatgtt caagatgcca gtatttacct gggcatcact
     1861 gtgcgcaaac gtactgatta ttgcttcctt cccaattctg acggttaccg tcgcgttgtt
     1921 gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg gcaacatgat
     1981 gatgtatatc aacctgattt gggcctgggg ccacccggaa gtttacatct tgatcctgcc
     2041 tgtattcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc tgtttggtta
     2101 tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg tcgttcatcg tctggctgca
     2161 ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta tcaccacgat
     2221 gattatcgcc atccctaccg gggtgaagat cttcaactgg ctgttcacca tgtatcaggg
     2281 ccgcatcgtg ttccattctg cgatgctgtg gaccatcggt tttatcgtta ccttctcggt
     2341 aggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc tgcataacag
     2401 cctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct tcggctgctt
     2461 cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg aaacctgggg
     2521 taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc cactgtatgc
     2581 gctgggcttc atgggcatga cccgtcgttt gagccagcag attgacccgc agttccacac
     2641 catgctgatg attgcagcca gcggtgcggt actgattgcg ctgggtattc tctgcctcgt
     2701 tattcagata tacgtttcta ttcgtgaccg cgaccagaac cgtgacctga ctggcgaccc
     2761 gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct ataactttgc
     2821 cgttgtgccg cacgttcacg aacgtgatgc attctgggaa atgaaagaga aaggcgaagc
     2881 gtacaaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg gtgccggtat
     2941 cgtcattgcg gctttctcca ccatcttcgg tttcgccatg atctggcata tctggtggct
     3001 agcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct tcgacgagga
     3061 cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc atttcgatga
     3121 gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg actgcccacg
     3181 cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat tttcggattt tggatctacc
     3241 tgatgagcga ctgcattctg ttctctatct tgtttgctac ctatgccgtt ctggtgaacg
     3301 gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt ctggttgaaa
     3361 ctttcttgct gttgttcagc tccatcacct atggcatggc ggctatcgcc atgtacaaaa
     3421 acaacaaaag ccaggtgatc tcctggctgg cgttgacatg gttgtttggt gccggattta
     3481 tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt ccggatcgca
     3541 gcggcttcct gtcagcattc tttgcgctgg tcggcacgca cggtctgcac gtcacttccg
     3601 gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg accagcacta
     3661 accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg gtttggatct
     3721 gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta acgcaagcag
     3781 cggcgcatcc catggcagcg taaagaccta catgacaggc tttatcctgt cgataatcct
     3841 gacggtaatt ccgttctgga tggtgatgac aggggctgcc tctccggccg taattctggg
     3901 aacaatcctg gcaatggcag tggtacaaat tctggtgcat ctggtgtgct tcctgcacat
     3961 gaataccaaa tcagatgaag gctggaatat gacggcgttt gtcttcaccg tgctaatcat
     4021 cgccatcctg gttgtgggct ccatctggat tatgtggaac ctcaactaca acatgatgat
     4081 gcactaagag cggcggttat gatatttaag caatacctgc aagtaacgaa accaggcatc
     4141 atctttggca acctgatctc ggtgattggg ggattcttgc tggcctcaaa gggcagcatt
     4201 gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc gtcgggttgt
     4261 gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac gaagaatcgg
     4321 gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac cttgctgggt
     4381 attgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg gctgggggtg
     4441 atgggctttg tggtttatgt cggcgtttat agcctgtaca tgaaacgtca ttctgtctac
     4501 ggcacattga ttggttcgct ctccggcgct gcgcctccgg tgatcggtta ctgtgcggta
     4561 accggcgagt tcgatagcgg cgcagcgatc ctgttggcta tcttcagcct gtggcagatg
     4621 cctcactctt atgccatagc cattttccgc tttaaggatt atcaggcggc aaacattccg
     4681 gtactgccgg tggtgaaagg catttcggtg gcgaagaatc acatcacgct gtatatcatc
     4741 gcctttgccg ttgccacgct gatgctctct cttggcggct acgctgggta taaatatctg
     4801 gtggtcgccg ctgcggtgag cgtctggtgg ttaggcatgg ctctgcgcgg ctataaagtt
     4861 gctgatgaca gaatctgggc gcgcaagctg ttcggtttct ctatcatcgc tatcactgcc
     4921 ctctcggtga tgatgtccgt tgattttatg gtgccggact cgcatacgct gctggctgct
     4981 gtgtggtaac acaacctctc agttaaaaag gtgctacggc accttttttc ttagcattag
     5041 aatcatatcc ctctcgaaat atttactaaa aaatccgcat gtttacccca ttcgtttgcc
     5101 gctttacact agtcgcgaat ttaaaacaga ggtggtaatg aacgattata aaatgacgcc
     5161 aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt tcattacgca tgctgggcat
     5221 gttcatggtt ctgccggttc tgaccacgta cggcatggct ctgcaaggtg ccagcgaagc
     5281 attaatcggt attgccattg gtatttatgg tctgactcag gccgtttttc agattccgtt
     5341 tggcctgctt tcagaccgca ttggtcgcaa accattaatt gtcggtgggc tggcggtgtt
     5401 tgccgccggt agcgttatcg ctgcgctctc tgactccatc tggggaatta ttctgggccg
     5461 ggcgctacaa ggctccggtg cgattgccgc agccgttatg gcgctgcttt ccgatctcac
     5521 gcgcgaacaa aaccgcacca aagcgatggc gtttatcggc gtgagctttg gcattacctt
     5581 tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc acgtgctgtt
     5641 ctggatgatc gctattctgg caacaaccgg cattgcgttg accatttggg ttgtgcccaa
     5701 cagtagcact catgtactta atcgtgagtc cggaatggta aaaggcagtt tcagtaaagt
     5761 gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt atgtgtctgc atattttgct
     5821 gatgtcgacc tttgttgccc tgcccggaca gatggctgat gcggggttcc cggcggctga
     5881 acactggaag gtctacctgg caacgatgtt aatcgccttt ggctcggtcg tgcctttcat
     5941 tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg tcgggttgat
     6001 cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac tggtggtcgg
     6061 cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc cttctgcctt cacttatcag
     6121 taaagaatcg ccagcaggtt acaaaggtac ggcgatgggt gtttactcca ccagccagtt
     6181 tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt ttgacggtca
     6241 gggagtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg ccagtaccat
     6301 gaaagaaccg ccgtatgtca gcagcttgcg cattgaaatc ccggcgaaca ttgccgcaaa
     6361 cgaggcgtta aaagtgcgtt tgctggaaac tgaaggcatc aaagaagtgt tgattgcaga
     6421 agaagaacat tcagcttatg tgaaaatcga cagcaaagtg acgaatcgct ttgaggttga
     6481 acaggcaatt cgccaggcat aaaagcgagg cgaaattgcc tgctgcgcta cgcttatcag
     6541 gcctactaag ttcctgagtt acaaaccttt gtaggccgaa tcaggcgttc atgccgcatc
     6601 tgacatgaac aaagcgcagg ttgtcgcgat taatcgcgga agtttttgaa ctggaacggc
     6661 tgacccagat cgccaccacg taccatcgcc atgacagcct gtaaatcatc acgagatttg
     6721 cccgtcacac ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     6781 atcattttga cgattttctt ctgagtcgcg ctctcaatgc cctgtttcag tttcgcttcc
     6841 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     6901 atgccgcgct tcagcaactt ggcacgcaga atatccagca actgattgac ctggaagtcg
     6961 gactcgctca acactttgat ggttttgctg gcgtcattca gctcaaatga ggcttcaacg
     7021 ttacggaagt caaaacggga ctccacttcg cggctcgcgt tatcgaccgc gttacgtgct
     7081 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     7141 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     7201 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     7261 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     7321 agggcaatta tggcttacag cactttgtaa acagggtcac gacgttcagg gctggctgcg
     7381 cgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaatga
     7441 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     7501 gaaagcatgg caggtttccg atgccgtcaa aagcctcgcg tccacactgc ctgtaaccac
     7561 gccaatactg ttaattcaca acggcatggg caccatcgaa gagttgcaaa acattcagca
     7621 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     7681 tgtggcaaac ggtatcacgc atattggtcc ggcacggcaa caggacggcg attacagtta
     7741 tctggcggat attttgcaaa ccgtattgcc tgacgtcgcg tggcataaca atattcgcgc
     7801 cgagctgtgg cgcaagctgg cagtcaactg tgtgattaat ccactgaccg ccatctggaa
     7861 ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt atgcagatat gcgaagaagt
     7921 cgcggcagtg atcgaacgcg aagggcatca tacttcagca gaagatttgc gtgattacgt
     7981 gatgcaggtg attgatgcaa cagcggaaaa tatctcgtcg atgttgcagg atatccgcgc
     8041 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     8101 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     8161 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     8221 ccactatcga cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgccagcg
     8281 atggtaacct ggcgattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     8341 tcgaagtggc tgatggcgaa tatgacgtga ttgtgctgcc tggtggcatc aaaggcgcag
     8401 agtgttttcg cgatagcacc ctgctggttg aaaccgttaa acagttccac cgttccgggc
     8461 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtcccgcac gatatcttcc
     8521 cgattggcaa tatgaccggc ttcccaacgc tgaaagacaa aattcccgcc gaacaatggc
     8581 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     8641 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     8701 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     8761 tgcggtaagc gtcgcatctg accagatgcg acgttacaac cacatattac gggcgataca
     8821 ccttcacatt gttaaagccc tgctcgcgca gatagagcgc ctgcaggcgg ctcatcaccc
     8881 cgcgctcaca ccacaacagc cagtttttgc tctggtcgag atcgccaaat ttggtgctca
     8941 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     9001 gttcatcgat agaacggata tcgaggatca cgtcgttcgg gccgaagcca ttgacggttt
     9061 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     9121 cttcctccac cactttatcg agaatgctga agtcgaactt ctcttcttcc gcttcaatct
     9181 tcgatttaac cgctttcacc gtcgggcttt tggaaatcac gccgcaatat tccggcatcg
     9241 tgcgggcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     9301 aagagatcag cggacgcagg atcagcgtat cggagacgtt atcaatcagg cgcaggttgg
     9361 tcagcgtctg gctggacacc tggccgagcg cttcaccggt aaccagcgcc tgtacgccgt
     9421 aacgttctgc cactttggac gcggcacgca ccatcatacg tttgaggata acgcccatct
     9481 gaccgtcgtc gattttctcg agaatttcac cgacgaccgg ctcaaagtta atggcgacaa
     9541 aacgcacgcg gtgggagcta ccaaaacggt tccacagata atgcgccacc tgacgaacac
     9601 caatttcatg cgccgcgccg ccgagattaa agaagcagta atgcacgcgg cagccgcgac
     9661 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacaacacat
     9721 cttcctgggt gccgatcggg aaaccgccaa taccttcgta gcggccttta atcagcagca
     9781 gacgatcgtc ttccacttcc agatggacag tcacttccgg attggtcagc ttcacgcgcg
     9841 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     9901 aatcatgttt gccgcggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     9961 gatattgaac caacgctttc tcgaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
    10021 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gccagacgct
    10081 ggttttcatc ttttgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
    10141 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
    10201 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
    10261 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
    10321 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
    10381 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
    10441 ttgagagtca gacattcatt atgccgaaga aaaatgaggc tcccgccagc tttgaaaagg
    10501 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
    10561 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gctaaattac
    10621 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
    10681 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
    10741 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
    10801 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
    10861 tgccactggt catatgttcg gcgttagcac aaacacactg gacgcacccg ctgccgccgt
    10921 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
    10981 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
    11041 tggtgacgct ttacaaacgc tggcgttctc gattctaagc gatgccgata tgccggaagt
    11101 gtcagatcgc gacagaattt cgatgatttc tgagttggcg agcgccagcg gtattgccgg
    11161 aatgtgcggt ggtcaggcat tagatttaga cgctgaaggc aaacacgtac ctctggacgc
    11221 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
    11281 tgcattaagc gccggagata aaggacgtcg tgctctgcca gtactcgaca agtacgcaga
    11341 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtgg gagatactgc
    11401 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
    11461 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcagtc
    11521 gctgaaacaa ctgactgaac agtcattcga tacctcggca ctggaagcgc tagcggacta
    11581 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
    11641 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
    11701 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
    11761 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
    11821 tgtctacaat accccgtttg accagttaat ctgggatgtg gggcatcagg cttatccgca
    11881 taaaattttg accgggcgcc gcgacaaaat cggcaccatc cgtcagaaag gtggcctgca
    11941 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
    12001 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
    12061 caccgtctgt gtcattggcg atggcgcgat tactgctggc atggcgtttg aagcgatgaa
    12121 tcacgcgggc gatatccgtc ctgatatgct ggtggttctc aacgacaatg aaatgtcgat
    12181 ttccgaaaat gttggcgcgc tcaacaacca tctggcgcag ctgctttccg gtaagcttta
    12241 ctcttcactg cgcgaaggcg gaaaaaaagt tttctctggc gtgccaccca ttaaagagct
    12301 gctcaaacgt accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgaaga
    12361 gctgggcttt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
    12421 gcttaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
    12481 tcgtggttat gaaccggcag aaaaagaccc aatcaccttc cacgccgtgc ctaaatttga
    12541 tccctccagc ggttgtctgc cgaaaagtag cggcggttta ccaagctatt caaaaatctt
    12601 tggcgactgg ttgtgcgaaa ccgcagcgaa agataacaag ctgatggcga ttactccggc
    12661 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gctactttga
    12721 cgtggcaatt gccgagcaac acgcagtaac ctttgctgcg ggtctggcga ttggaggcta
    12781 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    12841 tgacgtggcg attcaaaagc ttccggtcct gttcgccatc gaccgtgcgg gcattgttgg
    12901 cgctgacggt caaacccacc agggcgcttt tgatctctct tacctgcgct gcataccgga
    12961 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    13021 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcag ttggcgtgga
    13081 actgacgcca ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc gtggtgagaa
    13141 actggcgatc cttaactttg gtacgctgat gccagaagcg gcgaaagtcg ctgaatcgct
    13201 gaacgctacg ctggtcgata tgcgtttcgt gaaaccgctt gatgaagcgt taattctgga
    13261 aatggccgcc agccatgaag cgctggttac cgtagaagaa aacgccatta tgggcggtgc
    13321 aggcagcggc gtgaacgaag tgttgatggc ccatcgtaaa ccagtacccg tgctgaacat
    13381 tggcctgccg gacttcttta ttccacaagg aactcaggaa gaaatgcgcg ccgaactcgg
    13441 cctcgatgcc accggtatgg aagccaaaat caaagcctgg ctggcataat ccctactcca
    13501 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    13561 aggaaaaacc gaccttcgcg tttcccgact ttgcctcgga tgtatgacct ttggcgagcc
    13621 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca tcattaaacg
    13681 tgcgctggaa ggcggcataa atttctttga taccgccaat agctattccg atggcagcag
    13741 cgaagagatc gtcggtcgcg cactgcggga tttcgcccgt cgtgaagacg tggtcgttgc
    13801 gaccaaagtg ttccatcgcg ttggtgattt acctgaagga ttatcccgtg cacaaatttt
    13861 gcgctctatc gacgacagcc tgcgccgtct cggcatggat tatgtcgata tcctgcaaat
    13921 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    13981 aaaagtcggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    14041 agcactggaa ctacaaaaac agcacggctg ggcgcagttt gtcagtatgc aggatcacta
    14101 caatctgatt tatcgtgaag aagagcgcga gatgctgccg ctgtgttatc aggaaggcgt
    14161 ggcggtgatt ccatggagcc cgctggcgcg gggccgtctg acgcgtcctt ggggagaaac
    14221 caccgcacgg ctggtgtcgg atgaagtggg gaaaaatctc taccaagaaa gcgatgaaaa
    14281 tgacgcacaa attgcagaac gcttaacggg cgtcagtgaa gaacttggtg caacgcgagc
    14341 acaagttgcg ctggcctggt tgttgagtaa accgggcatt gccgcaccga ttatcggaac
    14401 ttctcgggag gaacagcttg atgaactgct aaacgcggtg gatatcacct tgaagccgga
    14461 acaaatcgcc gagctggaaa cgccgtataa accgcatccg gtcgtaggat ttaaataggt
    14521 atacaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    14581 aatacccagc ggccagtgat gtccaataaa gtacaggatg actgcggaga tcaccccggc
    14641 gacaatatcg tcaatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    14701 cggccacggc ttccacatat ccagaatacg gaaaatcaca aatccggcgg taacccactg
    14761 ccagtcattg gtcggcagcg ccatgagcgt aatccacata ccaataaatt cgtcccagac
    14821 aatactgccg tgatcgtgca cgcccatgtc tttcgccgtt tgatgacaaa gatagacgcc
    14881 gatacagatc cccagcatca ccaccagcga atagagctgc cagggcaaaa aagtcatcag
    14941 ataccagaag ggaatcgccg ccagcgaccc catcgtgcca ggaacgatcg ggcttaatcc
    15001 acttccgaat ccgacagcaa gcagatgcca cggattactc attttcaggc gacttttcgc
    15061 gacatcttta tggcgtggca aaatggtcat atcctttcca gtctaacgtg acaggttcgc
    15121 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    15181 gtacgcccag gtgtccgaga gccacatcca gcgcgccacg gttcagttcc ggcaccgtaa
    15241 aacacaactc gtaatcttcg ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    15301 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    15361 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    15421 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg tggcgacgga cggagatgac
    15481 gtttgatcaa gtagtccgca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    15541 ccagcccggc agcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    15601 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    15661 ataatggccc acgcgtggta tcgccgccaa taagttgcat atcgtaataa ttgagaagat
    15721 caaacaagct gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    15781 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    15841 gtgctttata agccagatca gcaggatcga tatcagggag gaagtggttg cccgccacca
    15901 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    15961 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    16021 tcagggagaa ctcgccacat gccatacgtt acgcctcagc agataaaaaa gaaaaggccg
    16081 gcgatcatgg aacggtcttc cgtgaatcta ccggcttgga tattactttt tgttagggcg
    16141 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    16201 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcgctacg
    16261 tttagacagc tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    16321 cagcagacgg gacaggtacg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    16381 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    16441 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    16501 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    16561 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    16621 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    16681 tcagaacacc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    16741 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    16801 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    16861 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    16921 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    16981 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    17041 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    17101 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    17161 tgacgtatct ctttgaattt aaattgggga gcgtcggcta atttctcaag ccctggcagc
    17221 gtgcataatc cacgggcgtc gttgcctaat agtttaggtg cgatatagac aatcagctca
    17281 tcgactaaac ccgcctgtag caatgcgcca gcgagcgttg gccccgcttc cacccagatg
    17341 ctgttaatct gctgtttacc cagttgcatc attagtacaa ccagatccag gtgaccttta
    17401 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    17461 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    17521 tggctatcaa tcacaatacg tactggctga cggagatttt gttgcgggta gagcccctga
    17581 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    17641 gcgctgctgg ttaaaatggc atgactttgc gcgcgcagtc gttgtacgtc gcgtcgcgcc
    17701 tgaggcgaag tgatccactg gctttcgcca ctcgccatcg ccgtgcgacc atcaagcgat
    17761 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    17821 ttcaattgct cagcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    17881 agacggtaaa gtccacgtcc agcaacctgc ggattggggt cttgcattgc agcaaccacg
    17941 cgcgccacgc ccgcagcaat taatgcatca caacacggtg gcgtacgacc atgatggcta
    18001 cagggttcga gtgtgacata cgccgttgcg cctttggctt tttcgccggc catgcgcaac
    18061 gcgtgtacct cggcatgcgg ttcacccgct cgttgatggt aaccttcacc gacaatttcg
    18121 ccatctttga caatgacgca cccgacattc gggttgggat gcgtggtaaa acgtcctcgt
    18181 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    18241 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    18301 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    18361 ttgctcggca cttcgcgctc accggtggcg cgcagctgcg attttatatg attgattgcc
    18421 atttcgacgt catcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgt
    18481 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    18541 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    18601 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    18661 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acagaaatct attttaccct
    18721 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcactgc
    18781 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    18841 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    18901 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaata
    18961 aagaaacggt caaaatcact cagcaaaaca agctgaatgc aaaatccagc agtggggttt
    19021 accttctgcc tggagcaaaa acaccagcaa gactggaaag ccagatcggt actttacgta
    19081 tgtcgctggt gaatattacg cctgacacag atggcactac cctgacgcta cgtattcagg
    19141 gcgaatccaa tgacccttta cccgcattca gcggaaccgt tgaatatggg caaattcagg
    19201 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaac gcccctgcca
    19261 gcgtcctcgc ccccagcgat gttgatattc cgttacagtt aaagggtatt tctgtggatc
    19321 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tattgcgggg
    19381 cgatattgtg cgtcccgcaa catctttctc cgtcattttg ttactccgct tacatcacct
    19441 ggattgatag taaaagtttg caacaagggt gaaagtcagt acaatccccg cccgaatgtg
    19501 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    19561 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    19621 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    19681 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    19741 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    19801 cacacccgtt tcggaccgca gctccgcaac gatacctacc ttgagtacga agcattcgct
    19861 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    19921 tccgatgcaa aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccgcgt
    19981 ttctccattg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    20041 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggccgcca gagcacctgg
    20101 tacatgggtc tgggtaccga catcgatact ggtctgccga tgagcctgtc catgaacgtc
    20161 tatgcgaaat accagtggca gaactacggc gcagcgaacg aaaacgagtg ggacggttac
    20221 cgtttcaaag tgaagtactt tgtgccgatt accgatctgt ggggcggcca gctgagctac
    20281 atcggcttca ccaacttcga ctggggttcc gacctgggcg atgacagcgg ttatgcgaac
    20341 aacggtatta aaacacgtac caataactct attgcgtcca gccatattct ggcgctgaat
    20401 tacgatcact ggcactactc tgtcgttgcc cgttactggc ataacggtgg tcagtggaat
    20461 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    20521 ggttatctgg tagtaggtta caacttctaa ttgtgagaat gccggatttc atatccggca
    20581 ttttttatca cttcttcttg ttcatcatcg ctttcaaatc agcgaatggg ttgtacttcg
    20641 cttcaccgac atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg
    20701 cttcggtgta tttcgaatgc tcatgatcgt ggcaatagag acaaagtaat tcccagttac
    20761 tgccatcttc cgggttatta gtatggtcgt gatcaatgtg gtgaacggta agttcacgca
    20821 ggttggaata aacaaactcg cgggaacagc gaccgcagac ccacggatag atttttaatg
    20881 ctttttcgcg atagccgctt tctaaccgcg catagttttt tgggatgata gccatgaaaa
    20941 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
    21001 tcaccttaca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat cagctgaaac
    21061 ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca
    21121 tgtgttcgcg cttcataccc agtttcagag ccagcgcaga tgccacatag atggaagatg
    21181 cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac
    21241 cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt
    21301 gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc
    21361 ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa
    21421 ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac
    21481 ccagcgtaat gataacgtcg tgcgccagcg caataacaac ccctgccgcc agtcgccact
    21541 caaagcggaa accaacgtac acgaggatag acagcagcgc cgccatcaac gccatcgcac
    21601 cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgccg
    21661 cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccac
    21721 cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt
    21781 gcagcatcgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag
    21841 ccggtttttc gagcgtaatt tcaataaccg taccaccggt gaaatccagc ccccagttaa
    21901 agccgcgcac gcccataata acgatagcgg cgattaacag cagaccagag atgccgaaag
    21961 cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag
    22021 tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca
    22081 tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca
    22141 ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg
    22201 atgactttaa tcagcgtggt gatgttcgca tcgaagatgg aactgaatgc gccacgataa
    22261 ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg
    22321 ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc cgcgatacct
    22381 ggcatactca gcgttgcgcc tggcaacagc gacataatac cgacaatcaa gatcaagttg
    22441 gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga
    22501 atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt ctgcataccc
    22561 agggttgggc caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca
    22621 cgcagcagca gtgaaagctg acgcgcttcg cttgggttat tgatgccagt aatacggaag
    22681 ctgttaccca gacgagactg gatgttggcg atattaatca cctcttcctg tttcaccaga
    22741 accgcacgac cgtttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc
    22801 atcggtttgc cgatgttgtc cttggtgaag ttagacatga tgttaccacc cgcgctatcg
    22861 agagagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata
    22921 tggtcaccgg tcagaattac gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt
    22981 ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc aacgttggtg
    23041 tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg
    23101 tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc
    23161 tccgccacgc caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca
    23221 tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg gttgctgccc
    23281 tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga
    23341 gctttagcat cgcggaaagt gatgctcagg ccgtagttgt tttctttacg aacggtggta
    23401 tacgggatgc ccttttcacg caggtcgctg cgcaggctat cgatattttg ttcctggagt
    23461 ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca
    23521 aggccgagct tcatcggctc agcatgaata gctgccagcc agcgcggcgt tgccggggca
    23581 agattaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc
    23641 agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac
    23701 ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg
    23761 gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga
    23821 agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga
    23881 taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg
    23941 cctttcggca gaacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc
    24001 agtgcgatag caatgtagcc gttttccgct actttggtta cgcgaccgac caggccaccg
    24061 ttcgtcagca cttcatcacc tttagcgatg gagtccatca gctttttgtg ttctttggtg
    24121 cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc
    24181 aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa
    24241 ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac
    24301 ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat
    24361 agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtgttgag
    24421 tcgcgcgcct aatatttcgt tgcaacggtc gagatgatgc aagtaagcgc gtgaataatt
    24481 gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc
    24541 attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt
    24601 tggcattacg cagtcaaaca tatcgatacc gcgacgtacg ccctcaacca ggtcttctgg
    24661 tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc
    24721 cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc
    24781 atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacact
    24841 gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct
    24901 acgcttcgcc caacgcagag acatctccat ggagcgtttt gcataatccc agtcagcagg
    24961 atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc
    25021 catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac ggaagtgaac
    25081 accctgttcg gtgattttac gaatatcacc aaggctgaaa acctggaagc cgccggagtc
    25141 ggtaaggatt ggtcctttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat
    25201 ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt
    25261 ggcttcaact tcttccggcg tcatcccttt tacggtgccg taagtgccaa caggcataaa
    25321 acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc
    25381 ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt
    25441 taaaccagcg ccgcggaatt actccccaac gcgctcatta attgcctgcg gattgtacgt
    25501 gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ccgctttata
    25561 ggcattcatg gtgtgttgat aaccagcaaa tgccgaaacc agcataatca gcgtcgattc
    25621 cggaaggtgg aagttcgtca ccagcgcatc gaccactttg tactggaagc ccggatagat
    25681 aaagatttgg gtatcgtcaa agaacggttc aatgagatcg tttttcgctg cctgagccgc
    25741 actttccagc gaacgtactg aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt
    25801 cgccgccagt accgcgtcta ccacatcctg cggcacttca gcgtattccg agtgcatgat
    25861 gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt
    25921 cacaaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt cgtcaaagtg
    25981 cagtccggcg gtcggtgctg ctaccgcgcc gggtttttcg ctataaaccg tttgataaag
    26041 ttcgcggtct gcatcttcgt ccggacggtc gatgtacggc ggcagcggca tatggccgat
    26101 gctgttgaga atatccagca ccgaacgttg atcattaaat tcgacttcaa acagtgcgcc
    26161 gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg cccagcagca gttctgcgcc
    26221 aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg
    26281 ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacagac gcgccgggat
    26341 cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga gtaaatcggt
    26401 gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact
    26461 gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaaag agaaatcggt
    26521 aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct
    26581 ctccctgcaa cctttacccc cctttagata gaatataatc gatgaatttt ttagctcacc
    26641 tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttactggct gatttcgtac
    26701 gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcggc
    26761 gaatcgacgt attaaccgac aatctgccag aagtccgcga agcgcgggag tggtttcgta
    26821 atgaaacgcg ccgtgtcgcg cctattacgc tggatgtgat gtgggatcac tttctgtccc
    26881 gccactggtc gcagctgtcg cccgattttc cgctacagga atttacctgt tatgcccggg
    26941 agcaagtgat gactattctt ccagactcac cgccacgctt tatcaatctg aacaactact
    27001 tatggtcaga gcagtggttg gtgcgctatc gcgatatgga tttcatccag agcgtgttaa
    27061 acggcatggc aagccgtcgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag
    27121 acgcccatta cgatgctctc gaaacccgct tctggcagtt ttatccgcgg atgatggagc
    27181 aggcgtcacg caaggcgtta taagacgtta attcatccac accgtagccg aaatggcagg
    27241 caaagccaga atcccgtccg tcagttgccc gtgaccttct ttgcgttgcc attgcgcgac
    27301 attaagcaac ggcgacgcgg gtagcaccac ttcacaggct tcgccacggt tgattgccac
    27361 cagtacacgc tgctgattca gcacgcggac aaataccacc acgttatctt ccgcatacag
    27421 cacctgacag ccgccacgac gcaacgcctg acttttctta cgcagcgcaa tcattcgctg
    27481 gtacagcgcg aataacgccg aatcctgctt ttccacctgc caggggaacg gcttacggca
    27541 aaacggatcg tttttgccat ccagtcccac ttcatcaccg taataaatgc acggtacacc
    27601 aggccaggtg aacagccaga ccaccgccag gggcaggcgc gcaacatccc gaccgagcag
    27661 cgttttaaat cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgttgttg
    27721 atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg
    27781 ctgcggatcg taagagatat cggtgttggc aagaaatccc cacaacggga atgtgaagcc
    27841 gcgatagttc atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa
    27901 atgttcgccg acaatatacg catccggctg ggtttctttc gccgcttcgg tgatcccggc
    27961 aacgtgctgc aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag
    28021 ccgccagcca tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc
    28081 gcgataaatt tcattcacca gactttccga ctgataatcc agcttcggca agctggcata
    28141 gcccagccag tcgagcgccg tgccatcatc actaaacgag taccagtcac gccagggtga
    28201 ttcggggttg tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga
    28261 atcgccactg tggttaaaca cgccgtccag caccagtcgt attcccagct gctgcgtatt
    28321 gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc
    28381 ctcggtatcg tatttatgca cgctgggagc tttaaacacc ggattgagat acagcgccgt
    28441 cacgccaagt tttttcagat acggcagttt ttcacttatc ccgtccagat cgccgccata
    28501 gaacgttgat ccgccagcct gcgccgtgac cggttcatcc cagtcacgca atatgatctc
    28561 ttgtccggct gcgtgatggt aatagacatg atcctgttca gcttcacgag gaagattacg
    28621 ggcgaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat
    28681 atccggtaca tcgatggcaa actgctcctg tcgtgccggc ggcattcggc tgaagccttg
    28741 cggtgtaaac caacgctggc gatcgtgcca cagcaatttg aaactataac gccgccgagg
    28801 ttgtccgcag gagagctcga tcgccgcccg ccatgcggtg acgccaggct gcggctgact
    28861 gcgctgctta tgcatcggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat
    28921 gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg
    28981 tttaacaaat gggggcaccg gcagatgcca tgcatttaac atcataattc ccctgtgatg
    29041 aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga aggatatttg
    29101 ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac
    29161 cggcttacag aacgcaagga ttactggttt tcagccagct gacgatcgtg gcggcgctta
    29221 aacatccagc caatcaacag cacaacaatc cacgcgaaac caacatacag cgagatacgc
    29281 gtatcagggt gataaccaat caacccgata ataaagagca ggaaaatcaa accgccgatg
    29341 gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa
    29401 cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccacac cgtggcgaag
    29461 gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc
    29521 gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgag
    29581 gttttgctga aaatttttgg cgcgctgccc tgctctgcca taccgtggag catacggcct
    29641 acaccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga
    29701 atgctggcgg caaaggtaat gcccatatgc tggaacgtca gcacaaacgg gctaccggca
    29761 gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac
    29821 accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc
    29881 gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc
    29941 aacgacatca ccataccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta
    30001 tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg
    30061 acaatcatga tgatgatagt ggcgacttta aagaacgaga accagaactc cagctcaccg
    30121 aataccttca cgctcatcag gtttacggcg caaatgatca gcaccacact cagtacccaa
    30181 atccagtgcg gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg
    30241 gcgatggcga caataaggat ttcaaagcag taggtccagc cggtaatgta acctgccagc
    30301 gggccgaggt tttcctgcgc ataacgcgag aaagagctgg ctgccgggtt atgtaccgac
    30361 atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac
    30421 aacacgcttg gaccggccat tttgatggca tctgccgaac cgtaaaacag cccggtgcca
    30481 attgctgaac ccagtgccat aaagcgtatg tgtcgggtac ttagcccacg ctttagcttg
    30541 ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag
    30601 ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct
    30661 gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc
    30721 agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca
    30781 gatgccttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc
    30841 acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac
    30901 ggcggataaa tggcggtcag caccggtaca gagatctgaa tcagctggct caagccgagg
    30961 ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt
    31021 acgtactggg cgaagaattc tgcacaagca caggtcaggc caaccgccgt gaccaggcag
    31081 gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt atgctgaacg
    31141 taagcatgca ggatcgccgc accgtttgca gactgatcga ccagcgacgc gctgtctgaa
    31201 cccagacgga acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc
    31261 cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta
    31321 acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttgacg
    31381 aagccgttag aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc
    31441 ggccagataa ttgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg
    31501 aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg
    31561 aaatagacca ggctgtaaat aaacagcggc agcgcggaat cacccgtcag cggcgcaatg
    31621 cccacttcaa aagaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg
    31681 taacaaactg ttgccagcag tacgccagcg actttaccaa ttggcgtgct gagactgtca
    31741 acaccgccgc caacttttgc cagcgccact accgttaata ccggtaggcc aacggcagta
    31801 atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgcaagcc gaccattgga
    31861 gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg
    31921 atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta
    31981 acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag
    32041 cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgtaaatgc
    32101 agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaagaag
    32161 ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt
    32221 tcaatgaaaa agacatggct aatcatgcga acaaaaaatc accaaccatt gtaaaatcta
    32281 acgcccgtat ttcgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa
    32341 gatgacaaag gcgaattaat cgctgttttt ggcaattaaa cgttccggga taataaaact
    32401 gaaacgtgta ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac
    32461 cgcatgtttc acgatcgcta accctaatcc gctaccaccg gtttgccggg aacgcgcttt
    32521 atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc
    32581 gttatcttca acactaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg
    32641 cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactacgtag
    32701 ctgatcttcg ttgcctgaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt
    32761 tttctgactc agagtctgag cctcgcgctc aacgacgcgc agcatcatcg gcacatcaac
    32821 cttttcattg agcaaatgcg tcggtgcggc ttctattttc gacagcgtca gcaattgctt
    32881 caccaaccct tccatccgct gggtctgctc acgcatggtg tgcaacgctt tttcacgtac
    32941 cgcgccttcc agcggttgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg
    33001 cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg
    33061 catttgcgtg acatctcgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg
    33121 aatctccaga tgccgcccgg tattgagcac cagattgagc gggcgagaga aatcacgcgt
    33181 tttcagatat tgcgtaaact ccgggtaacg cagcaagtta aggatgttct gcccgttatc
    33241 ttccggccag cgcaagccaa gaatctgttg cgccagaccg ttgcaccaga aaataccgcc
    33301 ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt
    33361 aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc
    33421 gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactac gatccaccca
    33481 cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga
    33541 tgccagcaaa aaccagggca ggtaacccaa aaatgcaccc aggatgaaag ccgggaggca
    33601 gcaaaatagc agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac
    33661 tccagttaag aaatcataag ccctgctcta cgtccgatga gcaaggcctt aaaagcgggt
    33721 tgaaaaacga taccctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc
    33781 cagtgcttta cgcaggcgac gaatgtggac atcgaccgta cgatcttcca catacacgtt
    33841 ggttccccat acgtgattta acagttgctc gcgactgtac acgcgctcgg gatgcgtcat
    33901 aaaaaagtgc agcagtttaa attcagttgg ccccatctcc agcggttctt cgcccgccat
    33961 cacccggtga gatgtcggat cgagacttag cccctgcatc tcaatcacct cttccaccgc
    34021 catcggcgat atacggcgca ttaccgcttt aattcgcgcc accagctcct tcggagaaaa
    34081 cggcttggtg atatagtcat ccgcaccggt ttcaaggccg cgcacgcgat cttcttcttc
    34141 ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg
    34201 tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg
    34261 ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa
    34321 gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac
    34381 cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg
    34441 acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag
    34501 gttattgatt tccgtggcac agaaaaaagc aaatggcaca tctgtttggg tataatcgcg
    34561 cccatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc
    34621 ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg
    34681 ctggagacag cacaagccca tctggtggat gcgattattg ttgccggtga tgttttcgat
    34741 accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag
    34801 caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat
    34861 gaatcgcgcg atatcatggc gttcctcaat actaccgtgg tcgccagcgc cggacatgcg
    34921 ccgcaaatat tgccccgtcg cgacgggacg ccaggcgcag tgctgtgtcc catacctttt
    34981 ttacgcccgc gtgacattat taccagccag gcggggctta acggtattga aaaacagcag
    35041 catttgctgg cggctattac tgattattat caacaacatt atgccgatgc ctgcaaactg
    35101 cgcggcgatc agcctctgcc catcatcgcc actggacatt taacgaccgt gggtgccagt
    35161 aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac
    35221 tttccaccag ccgactacat cgcgctcggg catattcacc gcgcgcagct tattggcggc
    35281 atggaacatg ttcgctattg cggctccccc attccactga gttttgatga atgcggtaag
    35341 agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg
    35401 aacgtaccgg taacgcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca
    35461 cagctggaac agtggcgcga tgtatcacag gagccgcctg tctggctgga tatcgaaatc
    35521 actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg
    35581 cctgtcgaag tattgctggt acgccggagt cgtgaacagc gcgagcgtgt gttagccagc
    35641 caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca
    35701 ctggaagaac tggatgaatc acagcagcaa cgtctgcagc atcttttcgc cacgacgttg
    35761 catagcctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga
    35821 actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc
    35881 tgtttgctat taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc
    35941 tggcgctgta tcacgaaact ccgcgcctct ctaacgtttc acaatcgcaa aatgatctca
    36001 tgacccgcga taccgccgaa tgtctggcgg aagtggagtt tgaagtcaaa gatgaagcgt
    36061 atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc
    36121 cacgcgtaga gctggcgcgc tgtgcagacg gcaaaattct cgccgacaaa gtgaaagata
    36181 agctggaact gacggcgacg ttaaccggac tggattacgg gcgcttcacc cgttcgatgc
    36241 tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcagaat
    36301 tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc
    36361 agcacaaatc ggcccgcaca gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt
    36421 tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag
    36481 aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc
    36541 tggacgaatt gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcattagccg
    36601 aagaagaaca agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc
    36661 ttcgtccgca ctgggaacgc atcgcagaac acagcacggc gctggcgcat actcgccagc
    36721 agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgtgcg agcattcgcc
    36781 accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg aatgcctggt
    36841 tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt
    36901 tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg
    36961 ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg
    37021 ctagcgccca ggcgcaacat gctgagcaac gcccactgcg tcagcgcctg gtcgcactgc
    37081 atgggcagat tgttccccaa caaaaacgtc tggcgcagtt acaggtcgct atccagaacg
    37141 tcactctgga gcagacgcaa cgtaatgccg cacttaacaa aatgcgccat cgttataaag
    37201 aaaagatgca acaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcattaaaa
    37261 cgctggaagc ccagcgcgca cagttacagg cgggtcagcc ttgcccactt tgtggttcca
    37321 ccagccaccc ggcggtcgag gcgtatcagg cgctggaacc gggcgttaat caggcccggc
    37381 tattaacgct ggaaaaagaa gtgaaaaagc tcggcgaaga aggtgcgacg ctacgtgggc
    37441 aactggacgc cttaacaaag caacttcagc gcgatgaaaa cgaagcgcaa agcctccgac
    37501 aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc aatatcaccc
    37561 tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc
    37621 tgcggttact cagccaacgg catgaattac aagggcagat tgccgcgcat aatcagcaaa
    37681 ttatccagta tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg
    37741 gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg acgcgtcagc
    37801 aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtatgc
    37861 agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgaactcccg cacagcgaag
    37921 aaactgtggc gctggaaaac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc
    37981 agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg
    38041 cgcagtttga caccgcgcta caggcgagcg tctttgacga tcagcaggcg ttccttgcgg
    38101 cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc
    38161 agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctgaca cagcatcagc
    38221 aacaccgacc tggcgggttg tctctcactg tgacggtgga gcagattcag caagagttag
    38281 cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc
    38341 tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa
    38401 tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg
    38461 ataaattccg caaatttgcc caagggctga cgctggataa tttagtccat ctcgctaatc
    38521 agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg
    38581 aagttgaggt tgttgatacc tggcaggctg atgcggtacg cgatacccgt accctttccg
    38641 gcggcgaaag tttcctcgtc agtctggcgc tggcactggc gctttcagat ctggtcagcc
    38701 ataaaacgcg tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa
    38761 cgctggatac cgcccttgat gcgttggatg ccctgaatgc cagtggcaaa accatcgggg
    38821 tgattagcca cgttgaagcg atgaaagagc gtattccggt gcagatcaaa gtgaagaaga
    38881 tcaatggact gggctacagc aaactggaaa gtgcgtttgc agtgaaataa ctattcagca
    38941 ggataatgaa tacagagggg cgaattatct cttggcccga ctggtcgtta tcctgcaagc
    39001 tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa
    39061 aaaagttatt ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat
    39121 tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ccgccgggca
    39181 tatgatctcg tattatgcgc tgggggtggt ggtcggtgcg ccaatcatcg cgcttttttc
    39241 cagtcgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa
    39301 cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtttccgg
    39361 cttcccgcat ggtgcatttt ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc
    39421 cggcaaagtc accgccgccg tggcagggat ggtttccggg atgacagtcg ccaatttgct
    39481 gggcattcca ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttgtt
    39541 gatcgctatt tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg
    39601 cgacgaggcg aaaggaaaac tacgcgaaca atttcacttt ttacgcagcc ctgccccgtg
    39661 gttaattttc gctgctacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt
    39721 aaagccatac atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat
    39781 gttagttggg ctagggatgg tgctggggaa tatgctaagt ggcaggattt caggacgtta
    39841 ttcaccactg cgcattgcag cagtgactga ctttatcatt gtactggcac tgctgatgct
    39901 ctttttcttc ggcggtatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg
    39961 attatttgcc ctttcagcac ctctacaaat attattactg caaaatgcca aaggcggaga
    40021 gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata
    40081 ttgcggtggt atgatgctga cgctggggct ggcatataat tacgtggcgc tgcccgccgc
    40141 cctgctttcg tttgctgcga tgtcgtcgtt gctgctgtat ggtcgctata agcgccagca
    40201 agcggcggat agtccggtgc tggcgaaacc actggggtag attgcagtca cggtgcacgt
    40261 tgcttattgc cggatgcggc gtgaacgcct tatccggcct acgggactgt tcattttgta
    40321 ggcctgataa gacacgccag tgtcgcatca ggcatcggag caccgggggg ttgccgccac
    40381 tgtgcgcgtt atttgttgcc ggatgcggcg cgagcgcctt atccggccta ccggacggct
    40441 cactttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatac gccaattgcc
    40501 tgggtttttt actcttgtgg ccataaccat gcagcgccgc gtacgccgct cgaatcacca
    40561 tgtttcgcct tgtgtaccgg cgtttcacat tctccgccga agacaaattg tttaatcaac
    40621 tgcggaaccg tttgatataa acggtctaca ttgctcattc cgccgcccag gacaatcaca
    40681 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    40741 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    40801 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc tgaaataaag
    40861 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    40921 tcttcgtcca tccacggtag cggattgtgt ccccactcgc ctgccgtgcc attgccgccg
    40981 atatgcgccc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    41041 aaaaccgtct gcgctcccgc tgccgcgcca tctaccgctt ctgaaaccgc cagacagttg
    41101 gcgtcatttg ccagccgcac ttcccgctgc aaccgcgcgc ttaagtcttt atcgaatggc
    41161 tgaccgttga gccaggttga attggcattc ttcaccacgc cggtgtaggg cgaaatcgaa
    41221 cctggaatgc ccatacctac cgttccgcgc tgccctgttg cctgctccgc catatcaacc
    41281 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    41341 cggtacaact gctcccctgc atcgcccaat gcaatcactt cagttttggt gccgcctaaa
    41401 tcgataccta tacgcacggt actctcctta tttttttcaa tatcaatagc gtagagacgg
    41461 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    41521 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    41581 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    41641 ccatgcggca gccaggacat ggcgaagatg ggctgggtac ctccgatggg atcgcacagc
    41701 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gtgcgcgcaa agaagaaaaa
    41761 atcctcccgt ccccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    41821 gaacaggtgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    41881 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggat cgatacggtt
    41941 aacggtttga ttatggtgga ctgcgccagc gccaaaaaag cggaagatac gctggcactg
    42001 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    42061 ctgacgctga ccgaatgggt acgttccggc agcgcggcac agggcttcca gctgcttgat
    42121 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    42181 ctgaccagcg aagagatcac caaccacatt gaagccggaa aagtggtgac taaactggcg
    42241 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctg
    42301 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    42361 cgctttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    42421 attgaaggat taggtggcga agcacaacgt taattgctga tttaccttta atgccggatg
    42481 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggaatataa tcgttagcta
    42541 cagcgggcaa taattcttag cgcagcaata ttatgcgttt tatgctgtaa cttgctccag
    42601 gggcgttgtg tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga atttttcgcg
    42661 ttgccccggt tttcactaaa tcacgtaaca gcgaaagatc gggttgttcg acaaataaga
    42721 gtaatgatgg atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtgaattat
    42781 taatagccag cgaccgctcc tgaggctcag tcaatgtcgt taacaacaac accggggtat
    42841 gtgcgttgtg caatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc
    42901 accattcatc atctgattta tcttttatcg tcgccagttc cgcaacaata tctggagatc
    42961 ttcgctgata ttgatatcaa ccttgatgaa ttatgaatat atcggaaatc gaaaaaatcc
    43021 cagcaaagga aatattatta tgggttaaag agaggacatt tcagaaaaat gccctcccgt
    43081 tcaaggggaa ggcgaggaat tacagatagc ggcacagata agaggtgggt tcggcaactt
    43141 gcagatgaaa ctcgctgtga ccgggaacat taaacaccga accggcttca tacacttgcc
    43201 agtcggtcgc gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttcag
    43261 gctcagcggt gctgaaggtg tattcacctt caaccataac acccacgctg gcgcgaccag
    43321 tgctgctgct ggaaaagccg atcgatttca ctttgccgga aaagtactca ttactttgaa
    43381 gcataaactg gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct
    43441 ggctgtcacg caaaattaca tcagtaattc cgcagccagc cgtgcaatca atacgttaga
    43501 gagcaagaca ggaacatcga gctgtttttg cagtaaatcg cgatgacgct ggttaaatcc
    43561 caaacaatcc agcatgatga catctgcacc ttttgccagt aattctttcc cggcatcaat
    43621 gattttttgt tccgaatcat gaatggggtt acccaatgaa aatactggcg gtttctgcaa
    43681 aatttgccat ttttgcgcct gaacggtcag caactcctca accggtacga taacccccac
    43741 ctgatgatct tcaacaatag aggaaaccag cggtggcaat atccgcgacg gctcaagaaa
    43801 gatcgtatta cgcgcggtca tactactaat gtttgctgta ctcattaata taatgacgtc
    43861 gtaaccctga ttatcgagca cttcaaccac accttgcagg tctcgctcaa ctttgcgacg
    43921 cgaaacatgg gccagatggt tgtcatttaa taatgtgaga attgtgtctt cgcctgcttc
    43981 tggcgcgtac tccgccatca cttcttcgcg gcttaacttc cccagcaggc tatgatggga
    44041 aatattatct tcgtcaatgt attctgtcag gagcggcaaa acttcctgca taggtacaat
    44101 gccgatagtc aggatcgcca acgacgcact cattgttctt ccaccttact tttatttcgt
    44161 cgttaattac cgggcaagtg ttaagcacca ctgctgacac ttacttgtgc tacaaatgac
    44221 aaaaagcgta gcagcagacc tgcactgcat aatgtaaaga ttccaaatga ttccagtaat
    44281 ggattgttat tgtttaatat tctaattatt tgaaaaacat gaattgtgaa aaatgtgacg
    44341 cagatccctt gtggcggcac tcagaatccc ttccgagcgg tctgatgcgg tcactctttg
    44401 tcagggtcct catagcgttt tttcgcatcc atcgcttcct gagcggtcgg atgttcactg
    44461 atcaacgagt ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct
    44521 ggctttcctt tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc
    44581 atcgttcttc cttatttcac gggatgaacg ttaagtatag gcgctcgaaa atcaacaatt
    44641 gatcgtctgt gccagagcgc tgcgaatttc agaaatcacc tggctgggtt cgtttgttgc
    44701 gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg
    44761 aacttcttcg ctcagtggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc
    44821 ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt
    44881 ttgcatgaag tgacgattaa attccgtcag aataatgccg ccgcccgtgg cgataacggt
    44941 ggatggcgca gttaccgctt ccagtgccgc cgtttctctg gcgcggaatc ccgcccactc
    45001 ttccctttcg acgatctccg cgaccgtcat attgagctgt gattgcaacc actggtcggt
    45061 atcgacaaac cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca
    45121 gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc
    45181 aatgcggcga aagccggtgt catgagaatg gcgatcatac catcaaacta gtacaatttc
    45241 gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa
    45301 taaatattca agaatgaagt gtaaataata aattacattt agccacgact acgttgcact
    45361 tccagccacc acttctccag ctccgcagca aaagcctggc ggtcacgttg tgaaagacta
    45421 tctggtcctc cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc
    45481 aggcgctcac gaatggtcgc tggcgtataa cgttcgccgc gcggattaag cgccgccgcg
    45541 cctttctcaa tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct
    45601 tcacactgcc gaacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta
    45661 cgaataaatc gtgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc
    45721 tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca
    45781 tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt
    45841 tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca
    45901 cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa
    45961 agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata
    46021 taccccctcc ccggataaag tcaccgccct gcatgaacag ttcggcatca acgccgcaga
    46081 atcggcgcaa gaagtagcgc aaatcgccga catcattttt gccgccgtta aaccaggcat
    46141 catgattaaa gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc
    46201 tattgctgca ggtatcacgc tcgaccagct tgcccgcgcg ctgggccatg accggaaaat
    46261 tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc gggatgacct ccgtaacgcc
    46321 aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg
    46381 cgaagcggaa gtgattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc
    46441 gccagcctac gtctttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat
    46501 gccacgcgcc caggcgtata aatttgccgc tcaggcggta atgggctccg caaaaatggt
    46561 gctggaaacg ggagaacatc cgggaacact gaaagatatg gtctgctcac cgggaggcac
    46621 caccattgag gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag tgatcgaagc
    46681 gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa tcctgatggc ttttgccgga
    46741 cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgctttgta gagcgccaaa
    46801 tctgccgatt tcaaccactc gcgatagtga ctcatttgtg gattcagcgg cgcaaccccc
    46861 acactaatcc gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt tagcccttca
    46921 tgcacccgta acatggcggt gatggcgctc tcagctggcg taccggacat gattactgca
    46981 aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt
    47041 cgggtaagcg ccacaatcgc ttcatcaccc acatcatggc cccaggtatc gttgatgctc
    47101 ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta
    47161 tcaaattcat tgcgtaacat agtttcccaa tgacgtctgt tatacacgcc cgtcataccg
    47221 tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttagt cgccgtctgg
    47281 tagctgaccc agccaaacag cagaggataa atgacaataa tgggaaggga gagccaccat
    47341 tccagcggtg cgctattgaa cgacacggtg atgcccgtca gttcgagggt gacaaggcag
    47401 gaaaccacca tcaacaccag acccgcaaca aacagacggg ggccgcctgc ccccatcaaa
    47461 ttcagacaca taatcatcaa catcgcggtg gaaggcagca cgtttacgcc cattacgcct
    47521 acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga
    47581 tcgacggccc tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc
    47641 aacaccagcc accaccagcc cggcggcggg tgcgaaacca gcgttgaagc aatcggtaag
    47701 aacatgccag ccagaccaac cgcgcggggt agtctgacgc gacgggcgaa gcgcagcccg
    47761 gaccgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc cgccgctttt
    47821 ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt
    47881 atagagacgg gactaaaggc agaagtatga aatttcgggt gagccatttt cgtagctcac
    47941 ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcactcata aacttattac
    48001 gatcatcccc tttcagagat tgttgtgttg cttgattatt acattcgcgc atcttttgct
    48061 gctgtggcgt caaacttttt tcaccaggcg cagatttgct gttcttcagg caatcactca
    48121 tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga ttacaagagg
    48181 tcatacgctg ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa
    48241 ccagaccgaa aagcaaggta accagtaatg taattttcat cgcaccatcc ctcttcatgt
    48301 tttaaccatg agcgtatgcg cccgtgatct gtcattaagt ctggttgcta acagcaaaaa
    48361 aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc cagggcggct
    48421 ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg
    48481 ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcggagttc
    48541 ccgtaactca tcaccatcac tgcgccatct ttggtattta gcgcctgggt gaggcccgga
    48601 gctttggtat ctggcgcaac tatctggctg gcatgggcgt gatcagcggt gactatgacc
    48661 aacgtgttac catccttttt agcgaattcc agcgcccgtt gtacggcttc atcgagatcg
    48721 accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc gattgacgca
    48781 ccttcaactt gcaggaaaaa gcctttctca tttttactca acaattcaat ggctttgtcg
    48841 gtcatctgcg ccagggtcgg tacactgtca ttacgttgcg gattaggcgt acaggtgact
    48901 gcgggcttat ctatattacc gtggtacgtt gctttcggtc cttgccagcg cactggcata
    48961 ttgccgtcag caaacagtcc taataggggt ttttgctgat tcgcttccgt caccgaattc
    49021 agtgaggcag catcgctcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt
    49081 tttccctgcc attcaccggc ggttgccgtt tcagcaaagg tttttgcacc gccgccaagc
    49141 gtaacatcgg cacgggcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc
    49201 agagcgttac ccggacattt ttcactggtc gcacttggac cgtagcattt gcgcgaggtc
    49261 acatgcgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg
    49321 gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata
    49381 tcgacgccca gcgcgccgtt ataggttttg acaccagttg accaggcggt tgctgatgca
    49441 gccgagtcgg tgacgtagtc cggtttgccg gtttttttat tcagcgcata gtgagtgtat
    49501 tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc ttcggcataa
    49561 ttgcgtgcgg cagtaatttc cgagtccccc atcccatcgc caatcagcaa aataatattt
    49621 tttgcaggtt tatcgctaag agaatcacgc agagcagcgg tctgatcacc cgttaagcgg
    49681 cgagcaccgc cgggtgcagt aatatcgccc tgagctgccc ggttttccag aacaggcatt
    49741 tctggtgtcc gggcttttgt cacaggggta aacagtaacg gtaagagtgc cagtgcaata
    49801 gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg
    49861 actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg
    49921 acgaggatgc ctcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg
    49981 aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg
    50041 ttgatcattt tgcgccatat tgcgaagcat tgccgtgacg ataatctcca aagcttctac
    50101 ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc
    50161 aatgagattt ttca
//
LOCUS       DACADA010000014        49795 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.014, whole genome shotgun sequence.
ACCESSION   DACADA010000014 DACADA010000000
VERSION     DACADA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.014"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            39..143
                     /locus_tag="ISB17_000815"
     CDS             39..143
                     /locus_tag="ISB17_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAP1978790.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            453..2651
                     /gene="speF"
                     /locus_tag="ISB17_000816"
     CDS             453..2651
                     /gene="speF"
                     /locus_tag="ISB17_000816"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAP1978791.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2648..3967
                     /gene="potE"
                     /locus_tag="ISB17_000817"
     CDS             2648..3967
                     /gene="potE"
                     /locus_tag="ISB17_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAP1978792.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4024..5664)
                     /gene="pgm"
                     /locus_tag="ISB17_000818"
     CDS             complement(4024..5664)
                     /gene="pgm"
                     /locus_tag="ISB17_000818"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAP1978793.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5690..6235)
                     /gene="seqA"
                     /locus_tag="ISB17_000819"
     CDS             complement(5690..6235)
                     /gene="seqA"
                     /locus_tag="ISB17_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAP1978794.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6420..7184
                     /gene="ybfF"
                     /locus_tag="ISB17_000820"
     CDS             6420..7184
                     /gene="ybfF"
                     /locus_tag="ISB17_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1978795.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7255..7617
                     /gene="ybfE"
                     /locus_tag="ISB17_000821"
     CDS             7255..7617
                     /gene="ybfE"
                     /locus_tag="ISB17_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAP1978796.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7757..8287
                     /gene="fldA"
                     /locus_tag="ISB17_000822"
     CDS             7757..8287
                     /gene="fldA"
                     /locus_tag="ISB17_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAP1978797.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8497..8583
                     /gene="uof"
                     /locus_tag="ISB17_000823"
     CDS             8497..8583
                     /gene="uof"
                     /locus_tag="ISB17_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAP1978798.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8576..9022
                     /gene="fur"
                     /locus_tag="ISB17_000824"
     CDS             8576..9022
                     /gene="fur"
                     /locus_tag="ISB17_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAP1978799.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9106..9432)
                     /locus_tag="ISB17_000825"
     CDS             complement(9106..9432)
                     /locus_tag="ISB17_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978800.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9482..10888)
                     /gene="chiP"
                     /locus_tag="ISB17_000826"
     CDS             complement(9482..10888)
                     /gene="chiP"
                     /locus_tag="ISB17_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAP1978801.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11330..12994)
                     /gene="glnS"
                     /locus_tag="ISB17_000827"
     CDS             complement(11330..12994)
                     /gene="glnS"
                     /locus_tag="ISB17_000827"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAP1978802.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13214..13975)
                     /locus_tag="ISB17_000828"
     CDS             complement(13214..13975)
                     /locus_tag="ISB17_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1978803.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(13977..14963)
                     /locus_tag="ISB17_000829"
     CDS             complement(13977..14963)
                     /locus_tag="ISB17_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HAP1978804.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(14956..16191)
                     /locus_tag="ISB17_000830"
     CDS             complement(14956..16191)
                     /locus_tag="ISB17_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAP1978805.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            16361..17518
                     /locus_tag="ISB17_000831"
     CDS             16361..17518
                     /locus_tag="ISB17_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP1978806.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            17522..18409
                     /locus_tag="ISB17_000832"
     CDS             17522..18409
                     /locus_tag="ISB17_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1978807.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            18469..19227
                     /locus_tag="ISB17_000833"
     CDS             18469..19227
                     /locus_tag="ISB17_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1978808.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            19263..20432
                     /locus_tag="ISB17_000834"
     CDS             19263..20432
                     /locus_tag="ISB17_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP1978809.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            20429..20899
                     /locus_tag="ISB17_000835"
     CDS             20429..20899
                     /locus_tag="ISB17_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAP1978810.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            20913..22193
                     /locus_tag="ISB17_000836"
     CDS             20913..22193
                     /locus_tag="ISB17_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane transport protein"
                     /protein_id="HAP1978811.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(22282..24228)
                     /gene="nagE"
                     /locus_tag="ISB17_000837"
     CDS             complement(22282..24228)
                     /gene="nagE"
                     /locus_tag="ISB17_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAP1978812.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24561..25361
                     /gene="nagB"
                     /locus_tag="ISB17_000838"
     CDS             24561..25361
                     /gene="nagB"
                     /locus_tag="ISB17_000838"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP1978813.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25421..26569
                     /gene="nagA"
                     /locus_tag="ISB17_000839"
     CDS             25421..26569
                     /gene="nagA"
                     /locus_tag="ISB17_000839"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP1978814.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            26578..27798
                     /gene="nagC"
                     /locus_tag="ISB17_000840"
                     /gene_synonym="nagR"
     CDS             26578..27798
                     /gene="nagC"
                     /locus_tag="ISB17_000840"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAP1978815.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27846..28598
                     /gene="nagD"
                     /locus_tag="ISB17_000841"
     CDS             27846..28598
                     /gene="nagD"
                     /locus_tag="ISB17_000841"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAP1978816.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28854..30518
                     /gene="asnB"
                     /locus_tag="ISB17_000842"
     CDS             28854..30518
                     /gene="asnB"
                     /locus_tag="ISB17_000842"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAP1978817.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(30560..30997)
                     /locus_tag="ISB17_000843"
     CDS             complement(30560..30997)
                     /locus_tag="ISB17_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978818.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            31331..31407
                     /locus_tag="ISB17_000844"
     tRNA            31331..31407
                     /locus_tag="ISB17_000844"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31365..31367,aa:Met,seq:cat)
     gene            31418..31502
                     /locus_tag="ISB17_000845"
     tRNA            31418..31502
                     /locus_tag="ISB17_000845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31452..31454,aa:Leu,seq:tag)
     gene            31526..31600
                     /locus_tag="ISB17_000846"
     tRNA            31526..31600
                     /locus_tag="ISB17_000846"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31558..31560,aa:Gln,seq:ttg)
     gene            31635..31709
                     /locus_tag="ISB17_000847"
     tRNA            31635..31709
                     /locus_tag="ISB17_000847"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31667..31669,aa:Gln,seq:ttg)
     gene            31725..31801
                     /locus_tag="ISB17_000848"
     tRNA            31725..31801
                     /locus_tag="ISB17_000848"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31759..31761,aa:Met,seq:cat)
     gene            31850..31924
                     /locus_tag="ISB17_000849"
     tRNA            31850..31924
                     /locus_tag="ISB17_000849"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31882..31884,aa:Gln,seq:ctg)
     gene            31962..32036
                     /locus_tag="ISB17_000850"
     tRNA            31962..32036
                     /locus_tag="ISB17_000850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31994..31996,aa:Gln,seq:ctg)
     gene            complement(32191..33366)
                     /gene="ubiF"
                     /locus_tag="ISB17_000851"
     CDS             complement(32191..33366)
                     /gene="ubiF"
                     /locus_tag="ISB17_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAP1978819.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            33512..34936
                     /gene="miaB"
                     /locus_tag="ISB17_000852"
     CDS             33512..34936
                     /gene="miaB"
                     /locus_tag="ISB17_000852"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAP1978820.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            35050..36129
                     /locus_tag="ISB17_000853"
     CDS             35050..36129
                     /locus_tag="ISB17_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP1978821.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            36126..36593
                     /gene="ybeY"
                     /locus_tag="ISB17_000854"
     CDS             36126..36593
                     /gene="ybeY"
                     /locus_tag="ISB17_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP1978822.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            36683..37561
                     /gene="corC"
                     /locus_tag="ISB17_000855"
     CDS             36683..37561
                     /gene="corC"
                     /locus_tag="ISB17_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAP1978823.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37586..39124
                     /gene="lnt"
                     /locus_tag="ISB17_000856"
     CDS             37586..39124
                     /gene="lnt"
                     /locus_tag="ISB17_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAP1978824.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSTAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYSRTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(39251..41128)
                     /locus_tag="ISB17_000857"
     CDS             complement(39251..41128)
                     /locus_tag="ISB17_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP1978825.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            41582..42490
                     /gene="gltI"
                     /locus_tag="ISB17_000858"
     CDS             41582..42490
                     /gene="gltI"
                     /locus_tag="ISB17_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAP1978826.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            42660..43400
                     /gene="gltJ"
                     /locus_tag="ISB17_000859"
     CDS             42660..43400
                     /gene="gltJ"
                     /locus_tag="ISB17_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAP1978827.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            43400..44074
                     /gene="gltK"
                     /locus_tag="ISB17_000860"
     CDS             43400..44074
                     /gene="gltK"
                     /locus_tag="ISB17_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAP1978828.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            44074..44799
                     /gene="gltL"
                     /locus_tag="ISB17_000861"
     CDS             44074..44799
                     /gene="gltL"
                     /locus_tag="ISB17_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAP1978829.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            44917..45852
                     /gene="rihA"
                     /locus_tag="ISB17_000862"
     CDS             44917..45852
                     /gene="rihA"
                     /locus_tag="ISB17_000862"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAP1978830.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            45936..47606
                     /locus_tag="ISB17_000863"
     CDS             45936..47606
                     /locus_tag="ISB17_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAP1978831.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(47666..49117)
                     /gene="djlC"
                     /locus_tag="ISB17_000864"
     CDS             complement(47666..49117)
                     /gene="djlC"
                     /locus_tag="ISB17_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAP1978832.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(49114..>49795)
                     /locus_tag="ISB17_000865"
     CDS             complement(49114..>49795)
                     /locus_tag="ISB17_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAP1978833.1"
                     /translation="FALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDL
                     IQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGE
                     GGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMHYFSAYSLGR
                     LYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPV
                     TLKDVSDL"
BASE COUNT        12128 a        12867 c        12499 g        12301 t
ORIGIN      
        1 accctacctt caactgacac agaaaaaggt aaaggtaaat ggaaaataat aaccgcttaa
       61 tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact
      121 ttcatttctt taatgcccgc tagtcttctg actaaagggc accccagcgt acaggtctcc
      181 ctgactttaa gcattacagg ttaatacctg tatccctcgg tgctcatata ctgctaaccc
      241 ttttaaactc taaataattc gagtcgcagc acatgcaact tgaagtatga cgagtatagc
      301 cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat
      361 cagcggtggg gttttgcttt tttccggcgt gatcaatttc tcctttgaga aattgaggac
      421 ctgctattac ctgaaataaa gagatgaaca aaatgtcaga attaaaaatt gccgttagcc
      481 gttcttgccc ggattgtttt tccactcatc gtgaatgcgt gaatattgac aaaagtaatt
      541 atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacatgga aaactcgatg
      601 aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat gaagaacgtg
      661 taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa tcacggaaag
      721 agttttatgg acgtcagtta gaaaccgctg ccagccatta tgaaacgcaa ctgcgcccgc
      781 ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt gactgcccgg
      841 gtcatcaggg aggtgagttt ttccgtcgcc atccggcagg caatcagttc gtggaatatt
      901 ttggcgaaat gctcttccgc tccgacttgt gtaatgccga cgttgcaatg ggcgatctac
      961 tgattcatga aggtgcgcca tgcattgcac agcaacatgc ggctaaagtg tttaatgccg
     1021 ataaaacgta ctttgtttta aacggcacat cctcctccaa caaagtggtt ttaaacgccc
     1081 tgctgacacc gggtgatctg gtgctgtttg accgtaataa ccataagtct aaccaccacg
     1141 gagcgttgct acaggctggt gcaacaccgg tttatctgga aacagcacgt aacccgtatg
     1201 gttttatcgg tggcattgat gcgcactgct tcgaagaaag ttatctgcgt gaactgatta
     1261 cagaagttgc accgcaacgg gcaaaagagg cgcgtccgtt ccgcctcgct gtgattcagt
     1321 taggcaccta cgacggtact atttataacg cccgccaggt ggtggataaa atcggccatc
     1381 tgtgtgacta catcctgttt gactccgcat gggtcggcta tgaacagttt attccgatga
     1441 tggcggactg ttcgccgctg ctactggagc ttaatgagaa cgatccgggt attctggtta
     1501 cgcaatctgt gcataaacaa caggctggtt tttctcagac ttcacaaatt cataaaaaag
     1561 acagccatat caaagggcaa cagcgttatg taccgcacaa acgcatgaac aacgccttta
     1621 tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat atcaacgcca
     1681 aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggtt aacggtatta
     1741 atgcccgcaa attgatcctc gataactgcc agcacattcg cccgttcgtt cctgaactgg
     1801 tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat ctgcgcttct
     1861 ttaaatttgt acccggtgag cactggcatt cttttgaagg ctacgcagag aaccaatact
     1921 ttgtcgatcc gtgcaaactg ttgctgacaa ctccaggtat tgatgcacgt aacggcgaat
     1981 atgaagcgtt cggtgtaccc gcaacgattc ttgctaactt cctgcgcgaa aatggcgtag
     2041 tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca gaagatatgg
     2101 ccaaactgca gcaacttgtt gccctgctgg tacgcttcga aaaactactg gaagctgatg
     2161 cgcctctggc ggaagtactg ccttccatct acaaacagca tgaagagcgc tacgccggtt
     2221 ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgtcac aacgtgaaac
     2281 aactgcaaaa agagatgttc cgtaaggaac acttccctcg cgtcagcatg aatccgcaag
     2341 aagccaacta cgcctactta cgcggtgaag tggagctggt tcgtctgccg gatgcagaag
     2401 gccgtatcgc tgccgaaggt gcgcttcctt atcctccggg tgtgctgtgt gttgttcctg
     2461 gtgaaatctg gggtggtgct gttctgcgtt acttcagcgc tctggaagaa ggcatcaacc
     2521 tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat gatggtcgta
     2581 agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcgccctg ttgaaagggg
     2641 aaaaattatg agtcaggcga aatcgaacaa aatgggtgtc gttcagttaa ccatactgac
     2701 gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg ccgaagtcgg
     2761 gacaatctca attatctcct ggctggtgac agccgtaggc tcaatggcac tggcatgggc
     2821 attcgcaaaa tgcggtatgt tcagccgtaa atcaggaggt atgggcggtt atgccgagta
     2881 tgctttcggt aaatccggta actttatggc gaactatacc tacggcgtct cgctgctgat
     2941 tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc tcggcgcgag
     3001 tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt gtaccgtggc
     3061 taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt ggggggtcat
     3121 tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga cgctttacgt
     3181 tgattcctgg aatccgcatc atgcaccctt cttcagtgcg gtaggttctt ccatcgctat
     3241 gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg tagtggaaaa
     3301 cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg cggtgattta
     3361 tatcgtctcc accaacgtga ttgccgggat tgtgccaaat atggagctgg caaattcaac
     3421 ggcaccattt ggtctggcct tcgcgcagat gttcacgccg gaagtgggta aagtcattat
     3481 ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca ccattgccca
     3541 ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc gtgtaaccaa
     3601 agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg ggttggcgct
     3661 gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc tggccgtggt
     3721 gactaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc agaaggtcgc
     3781 taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg gcgcgatgta
     3841 cagcttttat gcgctgtact catccgggga agaagccatg ctatacggtt ccatcgtgac
     3901 cttcctcggt tggacactgt atggtctggt ctcaccacgc tttgaactga aaaataaaca
     3961 cggttaatta agcaaagcaa aaaagggcga tcttccgacc gccctttttc ttatttgata
     4021 tgtttacgcg tttttcagaa cttcgctaac aatttcaacc gcttctttct caatctgctt
     4081 gcgatgttct tcaccaagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
     4141 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccga tagaagcgcc
     4201 attacccggt gccgccgtca gacgcgcggt gatcgggtca cctgccaggg tgctggcgct
     4261 caccatttcc ggagacagct tagacaacgc cgctttttgt gcggaagtcg ctgctgcctg
     4321 caaacggtta tagctcggcg caccaaagcg ttttgccagt tcgttatagt gttcctgcgg
     4381 gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt
     4441 atcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
     4501 gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccgggac
     4561 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag atgaaaccag
     4621 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtaattgat
     4681 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
     4741 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcaggcc
     4801 cgccatcgca cactcggagg agcagtccat acggatcgcg ccgtctttat caaggtgcat
     4861 aaagcggaag gtttgatcga cctgatcgtt aacaatagtc aggttgaggt tgtaatactc
     4921 gccaatacgc ttccagtatt cgataccgga accgcccagc ggatcaacgc caagcgtcag
     4981 gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca caaacggctg
     5041 caccagatcc tgctctttca catgaccgga tgccatcgct tcgtcgaggg agatacgctt
     5101 cacgcctttc aggccatcag ccagcagtgc gtttgctctg tcttccacca ctttagtgac
     5161 gttggtatca gccgggccac catttggcgg attgtacttg ataccaccat cttccggcgg
     5221 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
     5281 cagaatggca ttggaaatgg caggtgtcgg agtgaagcca ttgttttcct gcacaatgac
     5341 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc
     5401 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
     5461 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
     5521 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
     5581 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
     5641 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
     5701 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
     5761 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgta ccaggcacat
     5821 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa
     5881 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
     5941 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
     6001 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
     6061 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
     6121 atgcggcggt aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
     6181 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
     6241 tccagtgcag tgtcttagct gaatacaagt cgccaggagt atatgaataa atcccggcag
     6301 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
     6361 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
     6421 tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
     6481 ttgtccatgg tctgtttggc agcctcgaca accttggcgt actggctcgc gatctggtaa
     6541 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagatccgg
     6601 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca cagcagatcg
     6661 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggct cttactgcac
     6721 tagcccctga tcgcattgac aagctggtgg cgattgatat tgcgccggtc gattatcacg
     6781 ttcgccgtca tgatgagatt ttcgcggcca tcaacgcagt cagtgaatca gacgcacaaa
     6841 cacgccagca agcagcggca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
     6901 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccagta ttgtgggatc
     6961 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcatcct accctgttta
     7021 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
     7081 ttccacaggc tcgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
     7141 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
     7201 cccagcgtcc gcgtcgcttt ccgcgcgtgg acgttggcgt gactcatcgc gctgatgtat
     7261 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
     7321 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
     7381 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
     7441 aaccaactaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
     7501 gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta atatgagccg tagcgaactg
     7561 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
     7621 tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
     7681 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
     7741 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
     7801 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
     7861 cattgcaaaa agcagcaaag aagatctgga agcatatgac attctgctgc tgggcatccc
     7921 aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
     7981 gattgatttt aatggtaaac tggttgcgct gtttggttgt ggcgaccagg aagattacgc
     8041 agaatacttc tgcgatgcat tgggcaccat ccgcgacatc attgaaccgc gcggcgcaac
     8101 tatcgttggt cactggccaa ccgcgggcta ccacttcgaa gcgtcaaaag gtctggctga
     8161 tgatgaccac tttgtcggtc tggctatcga cgaagatcgt cagccggaac tgaccgctga
     8221 acgtgtagaa aaatgggtta aacaaatttc tgaagagctg catctcgacg aaattctcaa
     8281 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
     8341 aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccggtgttca
     8401 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
     8461 gacttgtagt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
     8521 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
     8581 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat
     8641 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
     8701 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
     8761 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
     8821 gacacagcaa catcaccacg atcacctgat ctgcctcgac tgcggtaagg ttatcgaatt
     8881 tagtgatgat tccatcgaag cgcgtcagcg tgaaatcgcc gcaaaacatg gcattcgcct
     8941 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
     9001 gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg caggttggct
     9061 tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact
     9121 gcgaatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg
     9181 cgttgcctga atgcactgct gataatccag tacgcggaca ctttcctgct cggcaaactg
     9241 ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc
     9301 cggcgaacct tgcgcggtgt tgatacaggc gctgtaagcc tctttcagac gactgtcttc
     9361 ctgtggcgcg gttgattgcg cacaagccac cagccccgat gccatcatgg cgatgagaat
     9421 cagttttttc atagcaaatt cctcaaacgt gcggcaggca caaaacctgc cgcatcgggc
     9481 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     9541 tgttgccata accaccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     9601 gcagtttgaa catcgtgcct ttggcgcggc cgtcctgaat ggtgtagacc gcatccaggc
     9661 tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga ttgctctgcc
     9721 aggtagcggg tttagcatcc catgcgtaaa cgtaggaggc gccgatggca aaacctggaa
     9781 gattccagtt tttcaggtca tacatcgcgc cgaagaagac cgctttttcg ccgttggcgt
     9841 tgaagtcaga acggttatcc caccagatat ccaggcgacc gttcgaggaa gcgtaggttg
     9901 gtgtcatacg ttgcaggaag tatccctgct gaccgtcggc tttaacccag gtgccttcca
     9961 gacgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcctgc agccaggcgg
    10021 tgccgtcata aagatcgttg gcgctgcgat cgtcaacttt atcgcgcgtg ccatagaact
    10081 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
    10141 attgatcgat atacccttcg gcctgaccaa acgccgcttc cagtacgaag ttatttttga
    10201 agtcgtactt cgccccaagg gagtgtagat aatcaacttt ggtggtttta tcgttctgat
    10261 aaaactcatc catttccaga tgccacggtg ctttgtattc gttggtccac atgtaggaga
    10321 aactcaacgc accagcatcg ccgtaatcaa aattcgcccc ggcctccgcc ccctgataag
    10381 tacccggcat aaagctccag tgtggcgcta acagcgtctg accggttggc tgaatataac
    10441 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc tgctttatac aggcttatac
    10501 cgcttttatc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
    10561 cgttcgggtg agagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcggcaatat
    10621 caaggccgaa catatcagca gcataaccag actgaaaatc gaggttggcg ttccaggtgg
    10681 agtgagaaag gttggttttg tatttatcgc cgtcggtaac atctttacgg tcgcgttcac
    10741 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10801 cctccggaat tgccataaag cccgacatag ctgtaacacc ggcgatagcc agcgccagcg
    10861 tactacgttt gccactaaac gcacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10921 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10981 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    11041 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    11101 cagttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    11161 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    11221 attaattcgc gaagaaaagc agaaaaaaac gccgcagagc gcggcgtttg ggataatttg
    11281 catatttctt gccggatgcg gcgtaaacgc tctatccgac aacgtaaact tactcgccta
    11341 ctttcgccca ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag
    11401 aatggcggct gtcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa
    11461 ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa
    11521 tcaccgacag gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcataca
    11581 aacggatttc aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt
    11641 tacgaccatc tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg
    11701 tgatattgcc ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca
    11761 gacgcacttc tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat
    11821 cggcgcgatc gatccaaatc tcaccgctaa atggcacctg acggctgccc atttccggtt
    11881 tattcggatg gttcggcatg gtaaccattt cgccttcgcc ctggtagttt tcgataacca
    11941 gtttcaccgg atcgataacc gccattgcgc gcggcgcatt ttcgttgaga tcttcacgga
    12001 tgcaggattc cagtgacgcc atctcaatgg tgttgtcctg cttggtcaca ccaatgcgtt
    12061 tgcagaactc acgaatagaa gccgcagtgt aaccacgacg acgcagacca gaaatggtcg
    12121 gcatacgcgg gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac
    12181 gcttggacat cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa
    12241 caggaatggt gatgttgtcc agtacccagt cgtacagacg acggttgtcc tggaactcaa
    12301 gcgtacacag agagtgcgta ataccttcca gcgcatcgct gatgcagtgg gtgaagtcgt
    12361 acatcgggta gatgcaccac ttgttgccag tctggtggtg ttcagcaaac ttaatgcggt
    12421 acagcaccgg atcgcgcatc acgataaacg gcgaagccat gtcgattttc gcacgcaggc
    12481 aggctttacc ttcttcaaaa ccgccggtac gcattttctc gaacagcgcc aggttctctt
    12541 caacgctgcg atcgcggtac gggctgtttt tacccggctg cgtcaaggtg ccgcggtatt
    12601 cgcggatctg ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga
    12661 tcgcataggc gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt
    12721 gaaaacctaa ccactctacg tcgtttttga tcgaatcgac atactcgata tcttctttta
    12781 ccgggttagt gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga
    12841 agttcaggca gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga
    12901 aacgggtgtg tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga
    12961 taaagttagt cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac
    13021 gtataacggc gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaaaa
    13081 tggttttgcg taagatattg gggcgttttc tgcgctactg ccagcagatc ctcgcgctgg
    13141 aaacgtaagg ccaaacgtcg ccagcctgac gcatttatta aatttccgga tgagctggcc
    13201 gcagagtcgt agattagtcg gctatgatta aacgcgtcga caaggacgca agcgttctac
    13261 gcgtctgccc atcaagctga ctatccgtaa taatctctgt cagttcatta agatgcgcga
    13321 tattaaacag cgaccatgaa ccgtattttg agctgtcagc caggagaatt ttgcgcgaag
    13381 catgcgcgat aagatcgcgt tttagtgcgg ctttctcttc cgtgggtgct gtaataccct
    13441 tttcaagatc ccagctgtta cagcttaaaa atgccacgtc cggccaggta ttttgcagca
    13501 atttacgacc atgttcgcca atgcaggact ggctggagtc gtcaatacgt ccgccgataa
    13561 tcgtcacttc aatttgcttg aattcagcca aaaaaagcgc gatatgcagg tcgcttgtga
    13621 tcacgcgaag cggtaaatgt gtaatctgcc gcgcaagttc aatcattgtc gtaccggcat
    13681 caaggacgac ggaatccccc gctttgacca ttgacgccgc tgcgctggcg atggcatgtt
    13741 tttccgccag gctgcgctgc attttctcat gggtagtcgg ttgggtagga ataaaccggt
    13801 tgagagtcac accgccgtgt gtacggctga taacaccctg ctcatcgagt tttatcaggt
    13861 ctcgacgaat cgtagccggc gacgcattgg ttaccgccac cagctcatct acggtgacca
    13921 gattatggct tttcaggtaa tccatgatct gctctaaccg gttatatccc ttcattttat
    13981 tctcgcgaaa tctgcattgc taactggatg gaaactgcca tgctctcaga cttggctttt
    14041 cctgtccagg caatatcaaa cgcggtgccg tggtcggcag aagtgcgaat aaatggcaac
    14101 ccggcggtga tattcacccc atcataaaat ccaagcagtt ttaaagggat atgcccctga
    14161 tcgtggtaca tagccactac catatcgaat agcccttcat ggcattgcat aaacacggta
    14221 tctggcgggc acgggccagt aacatcaata tcttcggcct gcattgcttc aatcgcaggc
    14281 gcgataatct cgatttcctc agtgccgaat aaaccatgtt cccctgcatg tggatttaca
    14341 cccgccacgg caatacgcgg acgctcaata ccaacacgtt tcaggaagtg gttcgcaact
    14401 cgaataaccg ttttgaccct ttcaccattc aaagtatcca ggaatttacg cagtgagatg
    14461 tgagtcgaaa tatggataac cttgagcttc tcggtataga gcaccatcgc gtactctttg
    14521 ctgccggtaa gatgggccag caattccgta tgccccggat agttatgccc gccaagatgc
    14581 agcgcttctt tattcagcgg cgcagtggct atggctttca cctcgcctga taaggccaga
    14641 gccgttgcac gtttgataca gcgatacgct aaatcgcctg ccacagcctg taccactccg
    14701 ggtgtcagcg catcggggtt agccagcggc tcatccatca cgttaatcac gccgggagca
    14761 aaatgggcgt cgcacacctt ttgaatgacg cgtaactctg cctgcggcgt gatattcatc
    14821 gccataatac gtcgcagcgt gcgcacacag cccaccacca ccaccggggc accagcaagc
    14881 ccggcttcgc tcagggattt aataatgatc tccgggccaa tacccgccgg atcgcccatc
    14941 gttaccgcaa taatattatt cactacgctt ctcctcgata aaattcagaa cctgatgcaa
    15001 cgtggtctca tcgccaaacc caccggcttt cgtcatcacc gaacgggacc agacgccgcc
    15061 cagaaagtga ccgtatggaa cgcattgcgc caccttcccc gtaatacgaa atccggtcgc
    15121 gcctaacgca ctggctgttg ccattgcgac atcaccacca gagagataaa gcgcatcggg
    15181 cattgcgcaa agcagaactt gccgggtaag ttcgccaaga aaccggcaaa ttttttcccc
    15241 aagtgcagcg cgactcatct gccaacgctg gcaaagggtg tcgatctgat gccttgctac
    15301 agaatcgtta caggtgtgga caatacagtg gtcgcctttc tgcaaggcct gcacaatacg
    15361 cgcgtcatag tcactggcat tgcctgccag aatgtgttca acatcgacgt atatctgtgt
    15421 gatacgtggg tgtgaatgaa gtgtggcgat ctgccgttga gcgatttcgc tcattgaacc
    15481 aataatggcc agcacggtgg gtggcggcat aatcgcggag cgccgggcaa cggcatcaca
    15541 aataccagcg gagccgacca gcagcagcgg ttgtttagcc tgcatcacag cggcagcgat
    15601 gcgatccagg tcgctgtccg tctgcgcatc tatcacgcca atccacggtt gctcataaga
    15661 caaatggctg agctgagaaa ccgtaacggg ctggcatgga atggccgtct gcaaccggat
    15721 gatgtccgca attcgggcgc tcaataccgg ggttttcgga tcgctagcaa actcggtttc
    15781 cgttatccgc acaccgttta cccaacactc accggccact gtggtccttc ccgcctgagg
    15841 aaatgcgggg gcaaccactg cgcccgttat accgcatgcc ttcatcaggg cttcaatttc
    15901 tgcgccgata ttgcctcgca gcgtggaatc tatcttttta atcacgagcg gcgggttatt
    15961 cgccagaggg agatgactca tcagcgaggt tattttcatc gccgccagct taggatccat
    16021 cgcccgactg tcgctgttaa tcacccatac gctggcatca ccgcgataag gcatttcaaa
    16081 ggccacatca actgtatgcc ccacctgcgc caggcttacc cccgcgtcgt tggctcccgt
    16141 gaaatcatcg gcaacgatga gaatgctcgg cttagtgatt cccttttcca tcatcactcc
    16201 aggttgtttt atgataacaa tgattatatt caatcatgat tgattaaatg tgagcaagct
    16261 caagttatta atcactcgaa taaattataa aattagacca tgttacgtag gctacggcat
    16321 tttgaatgat tgaattcaat caccagaaga gaggttattt gtggtaatta tcaatagcac
    16381 gatactgagc ggcgcaggcg ctatcccttc cctgacgtcg ctcttacccg acatcagaaa
    16441 aatgctgctg gtcactgacc gtaatattgc gcagctcgac ggtgtgcagc agattcgcgc
    16501 cttactggaa aagcactgcc cgcaggttaa cgttatcgat aatgtgcccg cagagcccac
    16561 gcatcatgat gtgcgccagc taatggatgc ccttggcgat gcctcttttg atgtggtggt
    16621 cgggatcggc ggtggcagcg tgttggatgt ggcgaagctg ctatcggtgc tttgccatcc
    16681 acaatcaccg gggctggatg cgctgcttgc gggtgaaaaa ccgactcagc gggtgcaatc
    16741 atggttgatt cctacaaccg ccggaaccgg ctcagaagcc acgccgaatg cgattctggc
    16801 aatccctgag caaagcacga aggtgggtat tatttcccag gtgctgttac cagactatgt
    16861 ggcgcttttc ccggaactga ccaccagtat gcccgcgcat attgcggcgt ccacgggcat
    16921 tgatgctctt tgccacttac tggagtgttt taccgcgacc gtggcaaatc cggtcagcga
    16981 taacgcggcg ctgactgggt taagtaaact tttccggcac attcaacccg ccgtgaacga
    17041 tcctcaggat ctgcgcgcaa aactggaaat gctgtgggcg tcttactatg gcggcgtagc
    17101 gataacccat gcgggcacgc atctcgttca tgcgctctcc tacccgttag gtggcaaata
    17161 tcatctgccg catggcgtcg cgaatgccat cttgctggcg ccgtgcatgg cgtttgttcg
    17221 cccctgggcg gtcgagaaat ttgcccaggt ctgggattgc attcccgatg cggaaaccgc
    17281 cctgagcgcg gaagaaaaat ctcatgccct ggtgacctgg ttacaggcat tagtcaatca
    17341 actcaagcta cccaacaatc tcgcggatct cggcgtaccg ccagaggata ttgcctctct
    17401 gagcgaggcg gcactgaacg tgaagcgcct tatgaacaat gtgccgtgcc agcttgatct
    17461 acaggacgta caggccattt accaaacact gtttccgcaa catcaattta aggaataaat
    17521 catgccgaaa accattgaag gtgtattaac cgctatcgtc accccgttta ccgcgaccgg
    17581 cagtctgaat atcccggcgt taaaagtgca ggtgaatcgt caactggaag cgggtaacgc
    17641 gattttttgc ggcggcacca acggcgagtt ttttgtgttg aatgaacagg agaagctcag
    17701 cgttacgcaa acctgcgtgg atgaagtggc tggtcgtgcg ccagtcgtgg cacacattgg
    17761 cgagatctct acgcgcgaaa cgattcgtct gggtcagcag attgaaaaac ttggcgtaga
    17821 tgccgtgtcg gtcattgccc cttacttcgt accgctgaaa caggaagagt tgattgcgca
    17881 ttacagcgca attgccgaca cactgagcat tccgatgttt ctctataaca tcccggcccg
    17941 cactggcaat acgatccagc cagaaaccgc gcgtgctctg gcgtcgcacc ccaatatcat
    18001 tgggattaaa gacagcgacg gaagcgacga aagcctgaag ggttttctcg atgcggtgcg
    18061 cgacattgac ggctttaatg tgctcaacgg cccggattcg ctgatccata aagggtttgt
    18121 cgagggatgt tcagcctgta tctctgggct cgccaacgtc gcgccgcgag aaatcaatgc
    18181 catctggtct cgcttccatg ccggtgatat tgaagggtct tatgaagcac aggagagtgt
    18241 aacgggcctg cgtaccgatc tttataaagt agcattctcc ccggctgcgg tgaaaaaagc
    18301 gttgcagctt atggggcatg atgttggtga tagccgctac gcggttaatt tcacgcctga
    18361 acagatcagc gagattcaac aaattattcg tcattataac attaattaaa ttaactctat
    18421 tatccagccc gctataaagt ttagataaag agaataaagg attaccccat gtttttgtgg
    18481 tttatgcagc cagatatctg gctcacttta attaccctca cctttctgga aattattctt
    18541 ggggtggata atattatttt tctttcactg gtggtggcaa aacttccgtt atcacaacaa
    18601 aatatggcgc gtaaactggg tttaagctgc gcgatgatta tgcggatatt attgctgata
    18661 tccattgcgt ggctttcaca tattacccag ccgcttttta ccgtgctgac gctggagatt
    18721 tcgttccgca cgttgattct gctcgggggc gggctgttcc tgatttataa agcgatacag
    18781 gagatacgcg aagagctgtc gcccgcgcag gaagacgaac atggcagcgg atcgcggcaa
    18841 ctgtcgttgt gcggtgccat tgtacagata atgctgctcg atatcgtgtt tagcctggat
    18901 tcggtgatca ccgccgtcgg cctttctcag catatattta tcatgattgc cgccgtaatg
    18961 attgccgtcg gtgtcatgat gtttgcggcg aaaacaattg gtgatttcgt taacgccacg
    19021 ccgtcaataa aaatactggc gttaaccttt ttattgtttg tcggtgtatt gctggttgcc
    19081 gatagtctca acattcatat tgccaaagag tatctctact tcgcgatatt tttctcatta
    19141 agtgttgaga cactcaatat tatccgtgaa agaaaaaata aaaaagtttc gacattgatc
    19201 gaaacgcatg aatcgcactc gcattaataa atctattacc aactttaaga tggagtctaa
    19261 ctatgtctgt agcccttatt cgtgacggcg tcatccgtcc atccgaaaac gattcgcatg
    19321 tgcttaccgc catgctgccc tcgtcctgcc cgcaaaacca cgcggcgaat attctgcctt
    19381 tgccggatgg cgcgttaatg tgcgtctggt ttgcgggaac gcaggaaggt attgccgata
    19441 tttctgtctg gggatcgcga ttgcccgctg gcggaatgca gtggagcgat gcggccaaac
    19501 tttcccacga tgacacgcgt tcagagcaaa acccggtgct gtttctcgcg ccggataacg
    19561 tgctgtggct cctatggacc gcacaaatat ccggtaatca ggacaccgcc atcgtccgct
    19621 accgtaagtc agacgacctc ggacaaacct ggggcgagat tgccaccctg cttgataaac
    19681 ccggtacgtt cattcgacag ccaatcacgg tgcttgataa cggcaactgg ctgctgcccg
    19741 tcttttattg ccgtacccaa cccggcgaaa aatgggtcgg caatgatgat atcagcgcag
    19801 tgaaaatttc cgctgacggt ggtcactcct ggcgcgatgt cgaggtgccg caaagtctcg
    19861 gctgtgttca tatgaatatc acaatgttgc acgacggtac gttggttgct ctctttcgca
    19921 gccgttgggc agacaacatc tatatcagcc attcagtgga caacggcgag agttggtcgg
    19981 tgccgcaagc aaccgaattg cccaacaaca attcgtctat tcaggtaacg acgcttgcca
    20041 gcggcgaact ggcgctggtg tacaacgcga tgagtgcggc aggcgcagta gaacgtcgcg
    20101 cgtcgcttta tgacgaaatc gacgatggcg acgacagccg caaagaaccg acagccgtcg
    20161 gacgctccgc tttctggggt gccccacgcg cgccgatgac ggtagcgatt tctgccgatg
    20221 gtgggaaaag ctggtcgtgg cggcgcaatc tcgatgaagg cgacggctac tgcatgacca
    20281 acaattccct ggaaaagcta aatcgtgagt tctcctaccc cagcatcaaa caaagcccgg
    20341 acggtacgct acatatcgct tacacctggt ggcgtcaggc catcaagtac gtacgcatct
    20401 cccccgagtg ggttaagggg caggcatcat gattatcggt aatctcaacc atttgtcgct
    20461 ggcggggctt cctgcctggg tgagaaatat tctgctgcgc ccggagtgtt cgttatcggc
    20521 gttatccacc agagaggacg gtcgctggca acctgaagga tgtcggtggt tctgctcgct
    20581 gggaacttca gacacgcaac ccgccgaact tcgccatacg gagtatcacc atctatgggc
    20641 cgatatacag gtggtgatta ctggttgtga gggcattaat gcgggaacgc ggcccatcgc
    20701 tcgcgaaaac gatgaagaac gtaagcccga tctgtttatc gccccatccc ctgaaaacag
    20761 tgtcgccatc accctacacg ccggtgattt cgccgtcttt atgcctgagg aacctcacca
    20821 ggcactgtgc gcaattggca cggctacttc aatacgcaaa gcggtcttta aagtaccgcg
    20881 cgacatgctg gaagcctaaa aggagagaaa aaatgacaat ggaaatgatc ctggcattag
    20941 gaatactggt cctgatgatc gtgctgatca tgtcggacaa aatgcctttt ggcgcaccgc
    21001 cactgcttgc gtgtttattg ctggttgtat cgggactttc gaccgttcaa caggcttttg
    21061 ccggattcgt caatccaagc gtggtgatga ttgcagggtt tatggtggtc atggcggcgc
    21121 tacaaaaaac ccggcttatc agcaacgtta aatctgcgat gataagcctg gtcaataaag
    21181 gcagttaccg cagctatggg ctgttgttag tcattgttat gctgggtgcc agtcttgccg
    21241 gcaccggagc gaccggttat tacgtgctaa ttctgtcgct ggtatccacc attccgtaca
    21301 gtaaaaagct accaacatcg aaattaatga tgcccctggg atttgccacc aaccacccgc
    21361 ttcttcctat caatctcgcg cttctctttg gcgttacggc gaccgtactc gaaaccgcgg
    21421 ggtttcatca ggcgatttcg atggggcgct ttgcgctggt gaatctcatc atgtcagcgg
    21481 ctttcctcgc atggagtctt attgcctacc gttttcttcc ggatcatccc attgcggatg
    21541 cttccgagga tgcgctggcg gcacgcgagg aaacgtttaa cgcccttcct gcctggaaag
    21601 aatattgcac tattgccgca ttcgctgtca gcgttatcgg catgatgcta atgaacattc
    21661 tgggaaatat cgccttcgtt attccgggtc ttgcgggtgc gtttgttctg atgatcgatg
    21721 tactggactt caaagaagtt cgcgatcata tgggggctcc ggtcatttta atgatggcgg
    21781 gggtgatcgg tgtagctgat gcactggcgg gcaccggctt tacggcgatg gttggcgatg
    21841 ccgttgcagg tgtgctcggt tctggcgtct caccgtttgt atttattgtc gcctttgctt
    21901 tgcttaccag tacctgcgcc accttcacgg gttcgaatat ggggtcagta tatatttttg
    21961 cccccatcgc cattgccgcc tgtactagcc ttgggcttaa cccgaccgct gccgcgattg
    22021 cggtggtgat ttctggctgg aatggcggct atatgcctat tgacggaatg ccagccatga
    22081 tcctcggaat ggggaaatac aaacttcccg aattctgggt gttctctgta ccaatgtatc
    22141 tcatccgcat tctggcgctt tgtgcaggcg ctgtttttat cttcccaatg taatctcaga
    22201 caaaaaaagc ggcagagaag atccctgccg ctttttattg ttggatgcga cgctgccgcg
    22261 tcgcatcaga cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac
    22321 aacatggccc tgagctttaa tgataaggcc gctgaaatca tcgatattgc tgcaaaccac
    22381 cgggctaatc atcgaacggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg
    22441 ttgccctgcg cttacctgcg cgccctcttc caccagacgt ttaaagcctt taccttccag
    22501 cgctacggtg tcgataccca tatggacgac gatctccgcg cctttttcgg tttccagaca
    22561 gaacgcgtgg ttggtgttga agattttcac gattgttcct gcggctggtg atacgacgat
    22621 tttatctgtc ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc
    22681 aggaatctga tccagtgcca caacatcccc ggtaatcggc gataccagct ccgcgataga
    22741 taccgcgttt ggtacagcct gcggttttgc tacaggagcg gcagttgccg gagttgcttc
    22801 agctgacgca gcggctaccg gaccacgggc aacgactttc ttcatcgcat cgccgatgga
    22861 ttctgctttc gcgccaacaa tcacctgaat agtctgtttg ttcagtttta ctaccccaga
    22921 agcacccaga cgtttacaca tcgcatcgtt gacgcgagct gagtcaacca cggtaaggcg
    22981 cagacgagta atacaggcgt caatggcttt caggttgtca gtaccgccaa ccgcagcaat
    23041 atagttggtt gccagttgat tgagaccttc ttcagtattg ctgttagcct cttcagtaac
    23101 gatctcgtct tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa
    23161 actgaacacc acgaagtaga tagcgaagaa gacaaccccc atcaccagca gcatccagac
    23221 gttctggctg gcggccggca ggttatacat caacgcgtag tcgatagccc ccgcagagaa
    23281 agagaagccg gcatggatac ccagcaacgt tgccacaaac aggctgatac cggtcagcag
    23341 tgcgtgcagg aggtacagca gcggtgcaag gaacatgaac aggaattcca gcggctcagt
    23401 cacaccggtc aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg
    23461 acgctctttc ggtgctgcga agtacatcgc cagcgccgca cccggcaggc cgaacatcat
    23521 aatcgggaag aagccggaca tgaacatccc cgcggtgccg tcaccggcat agaagcggtt
    23581 gatatcaccg tggaaaaccg ttcccgccgc gttggtgaat tcaccaatct ggaaccaggc
    23641 gatggtgttc agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa
    23701 gataccggaa cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg
    23761 ctgtaccggt ggccagacgt aaccaaaaat ggccgccagc accagacaga agaagccggt
    23821 ggctatcggc acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc
    23881 ggaccaacgg ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc
    23941 catgttaatt tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc
    24001 acccgccagt gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca cgccgatggc
    24061 gaatattaat gcgaggttat caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt
    24121 aagtaaatct ggctgaccga atcgcagcaa cagcgccgcc accggcagca ccgcgatagg
    24181 gagctgtaac gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct
    24241 acgagaaccc tatttggctc gtttaaagcc gtatttttat tttgctgcaa attgtactgc
    24301 cgatgttctg taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg
    24361 tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac caaatatcgt tatcatcact
    24421 cccttttact ggctaaacca gaaaacttat tttatcattc aaaaaatcag gtcggattga
    24481 cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct aatccgccaa cggcttacat
    24541 tttacttatt gaggtgaata atgagactga tccccctgac taccgctgaa caggtcggca
    24601 aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact gccgatcgtc
    24661 cgtttgtact gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg
    24721 aaatgcataa agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat
    24781 atgtcggtct gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct
    24841 tcgatcacgt tgatattcca gcagaaaata tcaaccttct caacggcaac gccccggata
    24901 tcgacgccga atgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt
    24961 ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg
    25021 cttctcgtac tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct
    25081 ttgataacga tgttaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacattgc
    25141 tggatgccga agaagtgatg attctggtgc tgggtagtca gaaagcactg gcactgcagg
    25201 ccgccgttga aggttgcgtg aaccacatgt ggaccatcag ttgtctgcaa ctgcatccaa
    25261 aagcgatcat ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat
    25321 atttcaatga attagaagca gaaaatatca agggtctgta attgttatcc ctgccctggc
    25381 tccttgctca gggcaatatt ttttaaaatc gggggtcaga atgtatgcat taacccaggg
    25441 ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct
    25501 gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa
    25561 cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca
    25621 gtttaacgac accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa
    25681 tgagaaatca ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat
    25741 gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaaa catccgaacc aggcgttagg
    25801 tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt
    25861 tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gagaacgccg acgtgattac
    25921 caaagtgact ctggcaccgg aaatggttcc agcggaagtc atcagcaaac tggcaaatgc
    25981 cgggattgtg gtttctgctg gtcactccaa cgcgacgttg aaagaagcga aagccggttt
    26041 ccgcgcgggg attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg
    26101 tgaaccgggc ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc
    26161 tgatggcctg catgttgatt acgccaacat tcgtaacgct aaacgtctga aaggcgacaa
    26221 attgtgtctg gttaccgatg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt
    26281 tgcgggtaaa acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag
    26341 cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gtggtatcgc
    26401 actggatgaa gtgctgcgta tggcgacgct ctatccggca cgtgcgattg gcgttgagaa
    26461 acgtctcggc acgctcgccg caggtaaagt agccaacctg actgcattca cacctgattt
    26521 taaaatcacc aggaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg
    26581 acaccaggcg gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg
    26641 gcggtttatc gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa
    26701 agtcagcttg cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg
    26761 atcaaagaag ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc
    26821 gaaacccgca atttccacgc aatcggcgta cggcttggtc gtcacgacgc caccatcacc
    26881 ttgtttgatc tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc
    26941 cagcagacgc tggaacatgc cctgctgaat gccattgctc agtttattga tagctaccag
    27001 cgcaagctac gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt agacccggac
    27061 agcggcaaaa ttcattacat gccgcatatt caggtagaaa actgggggct ggttgaagca
    27121 ctggaagagc gttttaaagt gacctgtttt gtcggccacg atatccgcag tctggcgctg
    27181 gcagagcact acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat
    27241 cgcggaaccg gggccgggat tatctctaac gggcgaattt ttatcgggcg caacggcaac
    27301 gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac
    27361 tttggctgcc tggaaactat cgccgccaat gctgccattg aacaacgggt gttgaatctg
    27421 ttaaagcagg gctaccagag ccgtgtgcca ctggacgact gcaccatcaa aactatctgc
    27481 aaagccgcga acaaaggcga tagcctggcc tcggaagtga ttgaatatgt cggtcgtcat
    27541 ctgggtaaaa ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgct
    27601 ggagaaatca ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc
    27661 caggcgctga aggcgtttcg tactaatcta ccggtggtac gttctgagtt ggatcaccgc
    27721 tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag
    27781 catttgctgg aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt
    27841 agtccatgac cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg
    27901 tcgccgtacc gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg
    27961 tgttgctgac caactatcct tcgcagaccg ggcaagatct ggcgaatcgc tttgccactg
    28021 ccggtgtcga tgtaccggat agcgtgtttt atacctccgc aatggcgacc gccgattttc
    28081 tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg attcatgaac
    28141 tgtacaaagc cggtttcact attaccgatg tgaaccctga ttttgtgatt gttggcgaaa
    28201 cgcgttccta caactgggac atgatgcata aagcagccta tttcgtcgct aacggtgcac
    28261 gctttatcgc caccaacccg gacacccacg ggcgcggttt ttatcccgcc tgtggcgcgt
    28321 tgtgtgcagg gatcgagaaa atctccgggc gcaaaccgtt ctatgttgga aagccgagtc
    28381 cgtggatcat ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa acggtgattg
    28441 tcggcgataa cttgcgtacc gatattctgg cgggcttcca ggccggtctg gagacgattc
    28501 tggtgctttc tggtgtttcg tcgctcgacg atatcgacag tatgccgttc cgcccaagtt
    28561 ggatttaccc atcggtcgct gaaatcgacg ttatctgaat agcaaccacg cctcagggcg
    28621 tggtttctta tttttaccca tcattaaaat caccccattc aataaaaccc gtcaaaaact
    28681 atcgatccat caataaatat acccaaaagc cctccccttt ttcatcaatc agcaatcgcc
    28741 attacgtttt ttatttttcc actgccaaat cgcttgcgca ccgtgctgct ttgcggcatt
    28801 tttttaacaa gtaaacacaa cacgcagtaa acaccaggtt aatggagaag gttatgtgtt
    28861 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg
    28921 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
    28981 acgccattct cgcccacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc
    29041 tctacaacca acaaaaaact catgtgctgg cagtaaacgg tgaaatctac aaccaccagg
    29101 cattgcgcgc tgaatatggc gatcgttacc agttccagac cgggtctgac tgtgaagtga
    29161 tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg
    29221 cctttgccct gtacgacagc gaaaaagatg catatctgat tggtcgcgac catctgggga
    29281 tcatcccact gtatatgggc tatgacgaac acggtcagct gtatgtggcc tcagaaatga
    29341 aagccctcgt gccagtttgc cgcacgatta aagagtttcc ggcggggagc tatttgtgga
    29401 gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgactac gatgcggtga
    29461 aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc
    29521 atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg tggtctggat tcctcaatta
    29581 tttccgctat caccaagaaa tacgccgccc gtcgcgtgga agatcaggaa cgctctgaag
    29641 cctggtggcc gcagttgcac tcctttgctg taggtctgcc gggttcaccg gatctgaaag
    29701 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
    29761 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
    29821 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
    29881 aaatggtgct gtccggtgaa ggttctgacg aagtgtttgg cggttatctc tacttccaca
    29941 aagcgccgaa cgctaaagag ttgcatgaag agacggtgcg taaactgctg gccctgcata
    30001 tgtatgactg cgcgcgtgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc
    30061 cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccacag gataaaatgt
    30121 gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgaagcg tatctgcctg
    30181 caagcgtggc ctggcggcaa aaagagcagt tctccgatgg cgtcggttac agttggatcg
    30241 acaccctgaa agaagtggca gcgcagcagg tttctgatca acagctggaa actgcccgct
    30301 tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgg gagatctttg
    30361 aagaattgtt cccgcttccg agcgccgctg agtgcgtgcc gggcggtcct tccgtcgctt
    30421 gttcttccgc taaagcgatt gagtgggatg aagcgttcaa gaaaatggac gatccgtctg
    30481 gtcgcgcggt tggcgttcac cagtcggcat ataaataaga cgtgtaatat tgccccggag
    30541 aaatccgggg caatttcagt tactggggcg cttgttgatt ctgatatgca gtaaggaaat
    30601 gttccgggtt tgccacatcg ctatttgaca actcatcact gtaggccacc aaccaggtcg
    30661 cctgctcatc cggaatatgc agtctgccat ttccctcttt caggcgtaat aactcaaaag
    30721 atttgccctt aattacggcg gtataggatt tctcaccttc ctgtaatgct ttcactttac
    30781 agcgaaatat ctgaccatcg atgtcattat caacatgcga tacctgaatg agggcttcat
    30841 tagaagcttt cgggcctatc cgcatcatcc acaccacagc gccttcctgc ccacgatagc
    30901 caaaaacata tttaccgatt tccggtttaa ccgtatcggc gaacgcagat tgcataagta
    30961 ataacgcacc catcaacgcc gtcataaaac gcagcatagt attccctcaa taaatcgcct
    31021 gaagtggtag cagagatgca ggcgtagcga tccctgccct gtcctgttgc aacgggccag
    31081 tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc ctgtatgaca
    31141 aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac gaatatagcg
    31201 ccatgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg
    31261 ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa
    31321 aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    31381 caggttcgaa tcccgtcgta gccaccatct tttttttgcg ggagtggcga aattggtaga
    31441 cgcaccagat ttaggttctg gcgccgcaag gtgtgcgagt tcaagtctcg cctcccgcac
    31501 cattcaccag aaagcgttgt acggatgggg tatcgccaag cggtaaggca ccggtttttg
    31561 ataccggcat tccctggttc gaatccaggt accccagcca tattcttcga gtaagcggtt
    31621 caccgcccgg ttattggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
    31681 ccctggttcg aatccaggta ccccagccat cgaagaaaca atctggctac gtagctcagt
    31741 tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc
    31801 aaattctgaa tgtgtcgaat atgttcggca aattcaaaaa ccaatttgtt ggggtatcgc
    31861 caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca
    31921 gccaatttat tcaagacgct taccttgtaa gtgcacccag ttggggtatc gccaagcggt
    31981 aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc cagccacatt
    32041 aaaaaagctc gcttcggcga gctttttgct tttctgcgta tattcaatgc cgaatacgat
    32101 gttgactcgt cttatcctta caaagtcgga tgcgacgctg ccgcgtctta tccgacctac
    32161 ggttggcatg catccagcaa tgttgtgagg ctacaaccct aacgcatatt tcagcgcctt
    32221 acgtttcaac acgccagcac gctccgccgc catcaaacca agattacgca caaaacgcag
    32281 cggtggcaga ttattgctga atccggcata aaacagatcc ataccacttt gcatcatgaa
    32341 gttatccgcc atacgccgca tctggtaacg cttgagcaca ggataactgg cccacgcttc
    32401 gccgtagctg cgggcgttca ccagaacgtc aatcagggca tcgacatcac gataaccaag
    32461 attcactccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagagcaag
    32521 ccctggttgc acatactgca acgcatggcg acgtataagc ggaaacgcac cagcggcaag
    32581 cggtgtcacg taacccagac gcgacgggaa atgcttcgcg atttccgcct gtagctgcgc
    32641 catattcata ttctgcaact ggcgaatgcg cgccggagag tcataccaca ccagcgatgc
    32701 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32761 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atacacgact gcgcatactg
    32821 ccaggcatga acgccaattc ccgccatctg tcgcacctgc gaatttgcgc cgtcggcacc
    32881 aatcaccagc ttcgcgagga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32941 atggcgatgc agtgcagtca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    33001 cagcgcctgc cacaaagcct gttgcaggac agtgttttcc accatgtagc caagcaacgg
    33061 tagcttaagt tcagcggcgt caaacacgac atgcgccgtt tcccactccc acgtttccag
    33121 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    33181 caatgatacc gaagccgcgc tgatcgccga gatccgcaca tccggttggc tgtcagcgac
    33241 aaacggcgca ggttctgcgt gctcgatcac cgttaccgta aacccgtgct gtgccagccc
    33301 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33361 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33421 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33481 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33541 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33601 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctgctgctga acacctgctc
    33661 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33721 agagaagaat ccagacctga ttatcggcgt cggcggctgc gtggcatcgc aagaaggcga
    33781 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcatcg
    33841 tctaccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33901 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33961 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    34021 tggtgaagag gtaagccgtc cgtccgacga tattctgttt gagatcgccc agcttgcggc
    34081 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    34141 ctacgacggc accaccggaa cgtttgccga tctgctgcgt ctggttgcgg cgattgacgg
    34201 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    34261 agtgtatcgc gacacgccgg agctggtgag cttcctgcac ttgccggtac agagcggttc
    34321 cgatcgcatt ctgaacctga tgggccgtac tcacacggcg ctggagtaca aagcgatcat
    34381 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca tcgttggctt
    34441 ccctggcgaa accaccgacg acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34501 cgacatgagc tacagcttta tcttctccgc tcgtccagga acgcctgccg ccgatatggt
    34561 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag agcgcattaa
    34621 tcagcaagcg atggcatgga gccgccgtat gctcggcacc acccagcgta ttctggtaga
    34681 aggtacatcg cgtaagagca tcatggagct ttccggtcgt actgaaaata accgcgtggt
    34741 taacttcgaa ggcacgccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34801 ctatccaaac tccctgcgcg gaaaagtggt gcgtactgaa gatgaaatgg gtctgcgcgt
    34861 cgcagaaaca ccggaatcgg tgattgcccg tacccgcaaa gaaaacgacc ttggcgtggg
    34921 ttattatcag ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt
    34981 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    35041 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    35101 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    35161 tttgatgaca acatcaaaca actcgaacgc cgtctcggca tcgaaatcaa tcgccgtgat
    35221 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    35281 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35341 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35401 tacggcaaag cagtcaatat caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35461 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35521 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35581 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35641 cctggcgatt taagccagaa agtagacccg tatctgcgcc cgctgtacga cgcgctgttt
    35701 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgca
    35761 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct tgatgagagc
    35821 cagaacacca ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35881 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaataccaa gtctggtttg
    35941 cgtcatgcca tcgaagtgct ggctgatgtc gaagagatca gctttaactt cttccacagc
    36001 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    36061 gaagccgaac aaaaacgtaa agcggcgctg gcggcagaac gcaagcgcga agaacaggaa
    36121 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    36181 cggaagagag ccagtttcag acatggctga atgcggtgat cccgcagttt caggaagaat
    36241 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36301 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36361 aaatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36421 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36481 tgttaggtta cgatcacatc gaagatgacg aagcagaaga aatggaagcc atcgaaacag
    36541 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36601 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36661 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36721 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36781 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36841 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36901 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36961 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    37021 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    37081 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    37141 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgtcattg acgaattcgg
    37201 tggggtttcc ggtctcgtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    37261 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37321 cgtgcgcgca ctggcttcta ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37381 tgaagaggtt gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcgcg
    37441 tggcgaaacc atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg
    37501 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata
    37561 aaaccgaaac tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat
    37621 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37681 cgtctggcct gcggcgatta tttcgctgat gggacttcag gcgctgacct ttaaccgccg
    37741 tccactccag tctaccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37801 taactgggtc tatgtcagca tcgcgacctt tggcggaatg cctggcccgg ttaacatctt
    37861 cctggtggta ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37921 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccgccc ctgctctctg
    37981 gcaagtgacc gagtttctgc gcggttgggt actaacaggc ttcccgtggt tacagttcgg
    38041 ctatagccag attgatggcc cgttaaaagg gctggcaccg ataatgggcg tggaagccat
    38101 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    38161 gcgtccgctg gtggtggccg tcgtgctgtt tgcccttccc ttcccgctgc gttacgtcca
    38221 gtggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcaatattcc
    38281 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctgaagattt actacaacgc
    38341 aacggcaccg ctgatgggca aatcatcgct gattatctgg ccggagtcgg cgataaccga
    38401 tctggaaatt aatcagcaac cgttcctcaa agctctggac ggtgagttgc gcgataaagg
    38461 tagctcgctg atcaccggga ttgtcgacgc gcgtctcaat aaacagaacc gctacgatac
    38521 ctacaacacc atcatcacac tgggtaaagg cgcaccgtac agctatgaat cagccgatcg
    38581 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccactggagt cgattctgcg
    38641 tccattagca ccgttctttg atttgccgat gtcgtcattc agccgcggac catatattca
    38701 gccaccgctg tcgttgaatg gcattcaact tactgcggcc atttgctacg aaatcattct
    38761 cggcgagcaa gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa
    38821 cgatgcgtgg tttggtaagt ctattggtcc atggcagcac ttccagatgg cgcgaatgcg
    38881 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38941 tggcccgcag ggtgagattc aggcgatgat cccgcagttc acccgcgagg tgttaaccac
    39001 taacgtaacg ccgaccaccg gactcacccc gtactcacgt accggcaact ggccactgtg
    39061 ggtgctgacg gcactgtttg gctttgccgc tgtactgatg agtctgcgtg ctcgcaaacg
    39121 ttaatgtgcc tgatgcgcga cgcttatcag gcctacgcga tttttgcaat atgttgaatt
    39181 ttcgcgattt tgtaggataa agcgtacttt ttcaacacgc tctggggcat gattatcatg
    39241 cccttttttc ttacggttca gggatacaag ccacatcgcc atattgggga tgccgaataa
    39301 ttgcctgcgg atcaagtgaa ctttctctgt ccagttgtat tagcccgttc ttcgccataa
    39361 caaatatctt tctaccatcc gctgaaaaac gaagatgatt cacgtagctg gcaggataaa
    39421 cgccaataat ctgcccggca ttaatattga gcaggatgac ataaccttgc aggccatatg
    39481 acaccaacgc taaatattga ccgtccgggc taataccctc aacggaataa acgctcctgc
    39541 tacaccattg cgtcataatg ctggcttgtt gatcctcagc tcttatacgt gaaatcccct
    39601 ggctatcggc aaaaataatc tcaccatttt ttggtcggaa tcccatcgca gaaacgacac
    39661 cacgcgcatc acctgcggga aagtcttctt tctgatgcgt gatggtattg atccgcacga
    39721 tgttacctct gcctgctccc ggaaccagta atgttttttc atccggggaa agtgccataa
    39781 cccaggcgcc tggtttaccg ctcatccccg tcccgtcgta tttctccagc gggatatccg
    39841 ccacatgctg ataagccacc agatcaatcg ctgacacact attactggtg cgattcatca
    39901 cccaggcttt tgtgccatca cgcgagagga taaggctgtc aggatgtgcg ccggtggtaa
    39961 tcgactgaat aatctccccc gttgccacgt taatgaccga taatgcattc gcttcaaatg
    40021 aagtcgcata cagcaacgtt tcatccgggc ttagcgccag cgacctgacg ccctgatctt
    40081 tacagccacc acaatgtttg actgactttt ctttcgctat gggggctgta aaggtatgca
    40141 ggattttctt ttgtccgagg tcgaaaacaa caatgctgtt cgtgttttca attgcggcat
    40201 acaacgtatt agcatcccga ctgatcgcca tatcatggat atcggcaatc tcagcgagta
    40261 aatcaccact ggcctgctca cgacttaccg gagcggataa tgtttccctg agggcgttgc
    40321 gctcctccac taactgctgc tttttttgtc gtcgctcttg ttcaatctcc tgcggataaa
    40381 acgcttcacg caggctttgc ctgacctgta aaactgattc atcaatctgc tgctgcacaa
    40441 gccagatagt cccggtcaga agaagagtga ccgcaacaat aatcccgcct tccgtaacga
    40501 gccgattttg cccaactaaa cgcgcactca gccaacccaa cgcgcccccc gcaatcaggc
    40561 cgccaatgtg gcaagcgtta tctattcctg tttgccgggt accatacaac agtgtcagcg
    40621 ccaccatacc caacagagga aatactcgcc gctggttttt atggtgtgtg cccgcacctg
    40681 aagcaagctg tgttgctatc gcagcgcccg ctattcccat aattgccccg gatgccccaa
    40741 cgctgatgac tatctgctca ctttcgcgta acgcataaca ggcgctgatc agcccaccaa
    40801 agatgccgga gaataaccag ataatcagga gtcgccattt ccccagtatc cgttccgcca
    40861 ctgtccccac caccaggagt gcaaacatat tcatcagcaa atgggaaaaa ctactgtgca
    40921 gaaaaacact gctcaataag cgccaggatt catccgacaa cgtgagagaa gcaacgttag
    40981 cgccgagatg gatgagtgcg gattgatcga cttcttcgta actcatccat tgtgcgaggt
    41041 aacaaaatat cccaacacag agcgcaaaaa ggatcagggt tattgcgggt aattgaacat
    41101 ttaacggctt aaccgaagat gcagacataa ggtgattctt ccttgaaaag actagtaaaa
    41161 agcattttcc cgtgtgtagt tatcatcccg gcaaacgtcc cacaccgaaa agttaacact
    41221 gtccgaaata tgactttttc aaactgaaat tacaatcgta attaattcac ccctaagtat
    41281 tttttggcat gtaagttgct ttatttactg tgtaagcaat gaaatcagcc gcaacgcaac
    41341 cgggttgcac caccacaaat cagcggtagc accaaaaagg tgcggtttaa gaattttgct
    41401 tcggaatggt gcggcgcacc tcgcaaaaac aaacaataaa acagcaatat ctttacattt
    41461 atccaaacta aatgttacct aacaagcaca acactgcaca ataaagtcgc agatgataac
    41521 aacacaaaca ctcacaacgg gtatccatgc gtttttaacg cagaagataa aggagttgga
    41581 tatgcaatta cgtaaacctg ccacagcaat cctggccctg gcgctttccg caggactggc
    41641 acaggcagat gacgccgccc cggcagctgg caatacgctg gacaaaatcg ccaaaaacgg
    41701 cgtgattgtc gtcggtcacc gtgaatcttc agtacctttc tcttattacg acaatcagca
    41761 aaaagtggtg ggctactcgc aggattactc caacgccatt gttgaagcag tgaaaaagaa
    41821 actcaacaaa ccagacttac aggtgaaatt gattccgatc acctcacaaa accgtattcc
    41881 actgctgcaa aacggcactt tcgattttga atgcggttct accaccaaca atgccgaacg
    41941 ccaaaaacag gcggctttct ctaacactat tttcgtggtc ggtacgcgcc tgctgaccaa
    42001 aaagggcggt gatatcaaag attttgccga cctgaaaggc aaagccgtag tcgtcacttc
    42061 cggcaccacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg
    42121 catcatcagc gccaaagatc acggtgactc tttccgtacc ctggaaagcg gtcgtgccgt
    42181 tgcctttatg atggatgatg ctctgctggc cggtgaacgt gcgaaagcga agaaaccaga
    42241 caactgggaa atcgtcggca agccgcagtc tcaggaagcc tatggttgca tgttgcgtaa
    42301 agacgatccg cagttcaaaa agctgatgga tgataccatc gctcaggtgc agacctccgg
    42361 tgaagcggaa aaatggtttg ataagtggtt caaaaatcca attccgccga aaaacctgaa
    42421 catgaatttc gaactgtcag acgaaatgaa agcactgttc aaagaaccga atgacaaggc
    42481 actgaactaa ttacaagaac caggggcgga aattccagcc ctctcgattg ttacgtagca
    42541 cggacagact atacgcctgg tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg
    42601 gtaacaatct tcgagggtag cagttaacgc tgctaccctc ttttttctgg agtagattta
    42661 tgtctataga ctggaactgg ggtatttttt tacaacaagc cccgttcggc aacaccacct
    42721 atcttggttg gatctggagt ggctttcagg tgacgatcgc tttgtcgatc tgcgcctgga
    42781 ttatcgcttt cctcgtcggt tcatttttcg gcattttacg taccgttcca aaccgttttc
    42841 tctccggtct gggtacgttg tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat
    42901 tctttacctg gtacctagtg atcccggagc tgctgccaga gaaaatcggc atgtggttta
    42961 aggcagagct ggatcccaat attcagtttt tcctttcatc catgctctgc ctggggctgt
    43021 ttaccgccgc ccgtgtttgc gaacaggtac gcgcagctat tcagtcgctg ccgcgtgggc
    43081 aaaaaaatgc cgcgctggcg atgggtctga cgctaccgca ggcttaccgc tacgtactgc
    43141 tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacctc agagatgatg aacctggtga
    43201 aaaactccgc catcgcctcg actatcggtc tggtggatat ggcggcgcag gcgggtaaat
    43261 tgctcgatta ctcggcccac gcctgggagt cgtttaccgc catcacgctg gcgtatgtat
    43321 tgattaacgc ttttatcatg ctggtgatga cgctggttga acgtaaagtt cgcctgcccg
    43381 gcaacatggg gggcaaataa tgtacgagtt tgactggagt tccattgttc cttccctgcc
    43441 atatctgctc gacgggctgg tgatcacgct gaaaatcacc gtcacggcag tggtgattgg
    43501 tattttgtgg ggcaccatgc tggcggtgat gcgcttgtcc agctttgcgc ccgtagcgtg
    43561 gtttgccaaa gcctacgtta acgtattccg ctcgattcct ttagttatgg ttttgctgtg
    43621 gttttacctg atcgtaccgg gttttctgca aaacgtgctg ggattatcac ccaaaaatga
    43681 tatccgttta atttcggcga tggtggcgtt ttcgatgttt gaagcggcct actattcaga
    43741 gattatccgt gctggtattc aaagtatttc tcgtggtcag tcgagcgccg cgctggcgtt
    43801 gggaatgact cactggcagt cgatgaaact gattattctg ccgcaggcgt tccgcgcgat
    43861 ggtgccgctg ttgctcactc agggcatcgt actgtttcag gatacctcac tggtgtatgt
    43921 gttatctctg gccgatttct tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca
    43981 ggttgagatg atcctgtttg ccggatttgt ttatttcgtt attagcctta gcgcgtcgtt
    44041 gttggtcagc tacttgaaaa gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa
    44101 tggtatggtc actttcaggt gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtt
    44161 gtggtggttt gcggcccgtc tggttccggc aaatcaacgc tgattaaaac cgtcaacggc
    44221 ctcgaaccgg tgcagcaagg tgaaatcacc gtcgatggca tcgtggttaa cgacaagaaa
    44281 accgatctgg cgaaactgcg ttcccgcgtc gggatggtgt ttcagcattt cgagctgttc
    44341 ccccatctgt cgattatcga aaacctgacc ctggcgcagg tgaaagtgct taaacgcaat
    44401 aaagcgccgg ctcgtgaaaa agcattgaaa ttgctggagc gcgtcgggct ttccgcacat
    44461 gccaataagt ttccggcgca gctttccggc ggccagcagc agcgtgtggc gatcgctcgc
    44521 gcattgtgca tggatcctat tgccatgctg ttcgacgaac caacgtcggc gctggatccg
    44581 gagatgatca acgaagtgct ggacgtgatg gtcgaactgg cgaacgaagg aatgacgatg
    44641 atggtggtga cccacgaaat gggctttgcc cgtaaagtgg cgaatcgggt gatctttatg
    44701 gacgagggta aaattatcga agactcgccg aaagacgctt tcttcgataa tccgaaatcg
    44761 gaccgcgcaa aagacttcct cgcaaaaatc ctgcattaat cactctggcg cgcatactca
    44821 attgcgcgcc atctcacgct ttctcgtttc gtccctcgta tctgttacat cccggcgtta
    44881 cccttgtcgc aaagaaacac acaacaagga gcaacaatgg cactgccaat tctgttagat
    44941 tgcgacccag gtcatgacga cgctatcgca atagttctcg ccctcgcctc accagagctt
    45001 gatgtcaaag caattacgtc ttccgccgga aaccagacac cagaaaaaac cttacgcaat
    45061 gttctgcgta tgctgacctt gcttaatcgc accgatattc cggtagcaag cggtgcggta
    45121 aaaccgttaa tgcgtaattt gattattgcg gacaacgtgc acggcgaaag cggcctggac
    45181 ggcccggcat tgccggaacc ggcatttgca ccgcaaaact gcacggcagt ggagctgatg
    45241 gcgaaaacgc tgcgtgaaag tgcggaacct gtcaccattg tttctaccgg accgcaaact
    45301 aacgttgcct tgctgctcaa tagccacccg gaactgcata gcaaaattgc ccgtatcgtg
    45361 attatgggcg gcgcaatggg gcttggtaac tggacgcctg cggctgaatt taatatttac
    45421 gttgacccgg aagcggcaga aattgtcttc cagtcaggaa ttccggtggt gatggccggt
    45481 ctggatgtta ctcacaaagc acaaatccac gttgaagaca ctgagcgttt ccgcgcgatt
    45541 ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt tcttcctcga atatcacaaa
    45601 gacgaaaaat ggggctttgt cggcgcacca ctgcatgacc catgcaccat cgcctggctg
    45661 ttgaaaccgg agttgtttac cactgttgag cgctgggttg gcgtggaaac acaagggaaa
    45721 tatacccagg gtatgacagt tgttgattat tattatctga ctggcaataa accgaatgcc
    45781 accgtaatgg tcgatgttga tcgtcagggc tttgttgatt tactggctga tcgtctgaaa
    45841 ttttacgctt aacatacgtc attgattaag atcaccacgc attacctggg tggtcttaat
    45901 ctggttatct ctttttcttt caagatataa atgcaatgga taatgcagaa cttgctattg
    45961 gtatcgatct cggtactacc aatagtttaa ttgccgtctg gaaagacggt gccgcgcaat
    46021 taattccaaa taagttcggt gaatatttaa caccatccat aattagcatg gatgaaaata
    46081 atcatatttt agtcggaaaa ccggctgtat cacggcggac ttcgcatccg gataaaacgg
    46141 cagcgttatt taaacgtgca atgggcagta ataccaactg gcggttaggc agcgacacat
    46201 ttaacgcgcc agaactgtcc tctttggtat tacgctcatt aaaagaagat gccgaagaat
    46261 ttctgcaacg tccgattaaa gatgtggtga tctccgttcc tgcttatttc agcgatgaac
    46321 aacgcaagca tacccgttta gcagcagagt tagccgggct aaatgcagtg cgcctgatta
    46381 atgagccaac ggcagccgcg atggcgtatg gcctgcatac gcagcaaaat acccgctcgc
    46441 tggtttttga tctcggtggc ggcacgttcg acgttacggt gctcgagtac gccacgccgg
    46501 tgattgaagt taacgcctcc gctggcgata acttcctcgg tggcgaagat tttacccata
    46561 tgctggtcga tgaggtttta aaacgcgcgg aagttgccaa aaccacgctt aacgatagcg
    46621 aactggcagc cttgtacgcc tgcgtggaag cggcaaaatg tagcaatcaa tcgccaatgc
    46681 acattcgctg gcagtaccag gatgaaatgc ggaaatgcga attttacgag aacgaactgg
    46741 aagatttgtg gttgccgctg ctcaatcgct tgcgagtgcc gattgaacag gcgttgcgcg
    46801 atgcacgtct gaagccgagt caaatcgaca gtctggtgct ggttggcggc gcgtcacaaa
    46861 tgccgctggt gcagcgaatc gcagtgcgtc tgtttggcaa attaccgtat caaagttacg
    46921 atccgagcac cattgtcgcg ctgggcgcag caatccaggc cgcctgccgc ttacgcaatg
    46981 aagatattga agaagtgatc ctcactgata tttgccctta ctcgttgggt gttgaagtta
    47041 accgtcaggg cgtatccggc attttctcgc cgattattga acgaaacacc acagtaccgg
    47101 tgtcgcgtgt agaaacttat tcaaccatgc acccggaaca ggattcaatt acggttaacg
    47161 tctatcaggg agaaaaccac aaagttaaaa ataacattct tgtggaatcc ttcgatgtgc
    47221 cgttgaagaa aaccgggact tatcagtcga ttgatattcg ctttagttat gatatcaacg
    47281 gcttgcttga agttgacgtg cttctggaag acggcagcgt taagtccaga gtgattaatc
    47341 acagtccggt aacattgagc gcgcagcaga ttgaagagag tcggacgcgg ttatccgcat
    47401 tgaaaattta tccgcgcgat atgctcatca atcgcacctt taaagccaaa ctggaagagt
    47461 tgtgggcgcg ggcgctgggt gacgagcgag aagagattgg tcgggtgatc accgattttg
    47521 atgcggcgtt gcagtcaaac gatatggccc gcgtggatga agttcggcgg cgggcgagcg
    47581 attatttagc cattgagatc ccataaacac ggctaccgga tgcggcgtaa acgctgcatc
    47641 cggcatcggc gcaaaaaaca catcgtcaac taaacaacat cgcgattttg ccgagagcat
    47701 aacctatcac cagcaccgtt aaactgattt tgataatgct ggtaatgctc tgtggttttt
    47761 catcctcatc atttatttct tcatggtttt caggtgttgg ctcatcgaca tcttcatcct
    47821 ccggctcgag cttcactttc gtcaacagca ttcgggtgat aatggtctct aatggatgtg
    47881 cctctccgcg ttccagccgc gcgtccagac gggccagcat ctcttgtttc ggaaacgctt
    47941 caagtactac ctgttccgct acccgataca aatccagtaa cggtgaatct tgctctgtct
    48001 cgctaccgac tctcgatttc caccacgccc atgctttgcg tttgaagaat aacgactcaa
    48061 taaacgattg acctaacggc gataattttc cgctatgtaa aatctccgcc cataacagcc
    48121 gcgtccgggc gctctcacca ggaacatatt tcaacggctt accttgcgca tctaccatgc
    48181 tccgcgcttc gataaccatc actaataacg cgaaataatc cgggcaatga cgcttgctcc
    48241 actgcaacaa caaattatcg gcttgtgtag atgggtaact ttcccggtac gcgcagagat
    48301 cggcaagcag gctttccccc tcgccgcagt acaaccgttg tgcgcactgg taataacggg
    48361 catcttcgct ttctggttct tccgcttgcc actgttgagc gacaggaatg agttccgcca
    48421 tacctgtttg caccgagctg taccagcgca acagtttgcg atgcaacttc gcatcatcag
    48481 gaatgaacca cggaccttcc atcgccaaaa acgcagtgac atagtcagga tggtaacggc
    48541 agcaacgttc cagcgagaag taatactcaa ccgtctggtt ctgcaacgca acgggatatt
    48601 ccagaaggct aaggaaatcg aaacaatcac cagcctgaat ggcctcaaga aaagcctccc
    48661 tttcttcctc atctattcct tcgcagtcat taaacgaatg ccagttcaaa cgctctgcca
    48721 gcaaagaagc acaatttaat gaaagatatc gcgcttctat agcgattgca cacagcggcc
    48781 agcgtaattg atcgacatcc tccatgttcc aggtatttaa ttgctgaata aatttctgcc
    48841 aggcggaggg ctgaaaacga tcattttctg aatccagtaa tgccctgaat acacgtagta
    48901 tttcatgctc agtggcagca tctttttctt catcatctgc ttcctcgaca ggatttaccg
    48961 ccagtcgcag cgcctcttca taggcctggc gtaatgcttt aaacccttgt ggatcggttt
    49021 cgggatgaca taacggtaag cgagcaagat aagcctggcg gataatgtct atttgcgtcg
    49081 tgctttcaat ttcgaggatt tgccaacatg ttttcatagg tcgctcacat ccttaagcgt
    49141 taccgggcat tcaggctgga cagttaatgg ctgccaggga agcgtggcat agaaacggtc
    49201 gcttcccgct gccagctctt cgaacatccg actcccggcg ctatcgtatt tgtacagggt
    49261 tagcgcctcg cgtagcgaag cgttgtcttc gcactgagaa gattgccagt agagacgccc
    49321 taacgaatag gcagaaaaat agtgcatcca gctgtgatag taatggtgtg ctcgtatata
    49381 gacgcgcgat tgcagccata aactctcgtc ttccgtcaac cacttgttga gcacaccaac
    49441 ccgtgataaa aaacccatcc gcacataatc ccaggctttg attccccctt ccccacaaca
    49501 catagcggtt tgggcaacga attgggtata aatcaaccct ctctccgagc cgccagcaat
    49561 taatgctttc cactcttcag gggaagagcg atgccatgtc agatataagc cagctaaatc
    49621 attggcatga ccatcatcgg tcatctgata aatagtctga attaaatcat cacgattatt
    49681 tattccccag gatgatgaga gatcgaattc ttttatcccg gtatacattt ttggcgagtc
    49741 atgcgcgggc ttgcaaccct gattaagtat ttccatgggc gtagaaagcg caaaa
//
LOCUS       DACADA010000015        48749 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.015, whole genome shotgun sequence.
ACCESSION   DACADA010000015 DACADA010000000
VERSION     DACADA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.015"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(132..836)
                     /locus_tag="ISB17_000866"
     CDS             complement(132..836)
                     /locus_tag="ISB17_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP1978834.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(973..1425)
                     /gene="moaE"
                     /locus_tag="ISB17_000867"
     CDS             complement(973..1425)
                     /gene="moaE"
                     /locus_tag="ISB17_000867"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAP1978835.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1427..1672)
                     /gene="moaD"
                     /locus_tag="ISB17_000868"
     CDS             complement(1427..1672)
                     /gene="moaD"
                     /locus_tag="ISB17_000868"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAP1978836.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1665..2150)
                     /gene="moaC"
                     /locus_tag="ISB17_000869"
     CDS             complement(1665..2150)
                     /gene="moaC"
                     /locus_tag="ISB17_000869"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAP1978837.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2153..2665)
                     /gene="moaB"
                     /locus_tag="ISB17_000870"
     CDS             complement(2153..2665)
                     /gene="moaB"
                     /locus_tag="ISB17_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAP1978838.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2687..3676)
                     /gene="moaA"
                     /locus_tag="ISB17_000871"
     CDS             complement(2687..3676)
                     /gene="moaA"
                     /locus_tag="ISB17_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAP1978839.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3664..3808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4073..4981
                     /gene="yvcK"
                     /locus_tag="ISB17_000872"
     CDS             4073..4981
                     /gene="yvcK"
                     /locus_tag="ISB17_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAP1978840.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5019..7040)
                     /gene="uvrB"
                     /locus_tag="ISB17_000873"
     CDS             complement(5019..7040)
                     /gene="uvrB"
                     /locus_tag="ISB17_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAP1978841.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7619..8296)
                     /gene="bioD"
                     /locus_tag="ISB17_000874"
     CDS             complement(7619..8296)
                     /gene="bioD"
                     /locus_tag="ISB17_000874"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP1978842.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8289..9044)
                     /gene="bioC"
                     /locus_tag="ISB17_000875"
     CDS             complement(8289..9044)
                     /gene="bioC"
                     /locus_tag="ISB17_000875"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAP1978843.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9031..10185)
                     /gene="bioF"
                     /locus_tag="ISB17_000876"
     CDS             complement(9031..10185)
                     /gene="bioF"
                     /locus_tag="ISB17_000876"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAP1978844.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10182..11222)
                     /gene="bioB"
                     /locus_tag="ISB17_000877"
     CDS             complement(10182..11222)
                     /gene="bioB"
                     /locus_tag="ISB17_000877"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAP1978845.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11309..12598
                     /gene="bioA"
                     /locus_tag="ISB17_000878"
     CDS             11309..12598
                     /gene="bioA"
                     /locus_tag="ISB17_000878"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAP1978846.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12657..13133
                     /locus_tag="ISB17_000879"
     CDS             12657..13133
                     /locus_tag="ISB17_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAP1978847.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13285..14568
                     /locus_tag="ISB17_000880"
     CDS             13285..14568
                     /locus_tag="ISB17_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAP1978848.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14709..16970)
                     /locus_tag="ISB17_000881"
     CDS             complement(14709..16970)
                     /locus_tag="ISB17_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAP1978849.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17153..18586)
                     /locus_tag="ISB17_000882"
     CDS             complement(17153..18586)
                     /locus_tag="ISB17_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP1978850.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18662..19714)
                     /locus_tag="ISB17_000883"
     CDS             complement(18662..19714)
                     /locus_tag="ISB17_000883"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAP1978851.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            19898..20851
                     /locus_tag="ISB17_000884"
     CDS             19898..20851
                     /locus_tag="ISB17_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978852.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(20892..21887)
                     /gene="pgl"
                     /locus_tag="ISB17_000885"
     CDS             complement(20892..21887)
                     /gene="pgl"
                     /locus_tag="ISB17_000885"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAP1978853.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22042..22860
                     /locus_tag="ISB17_000886"
     CDS             22042..22860
                     /locus_tag="ISB17_000886"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAP1978854.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(22861..23919)
                     /gene="modC"
                     /locus_tag="ISB17_000887"
     CDS             complement(22861..23919)
                     /gene="modC"
                     /locus_tag="ISB17_000887"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAP1978855.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(23922..24611)
                     /gene="modB"
                     /locus_tag="ISB17_000888"
     CDS             complement(23922..24611)
                     /gene="modB"
                     /locus_tag="ISB17_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAP1978856.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24611..25384)
                     /gene="modA"
                     /locus_tag="ISB17_000889"
     CDS             complement(24611..25384)
                     /gene="modA"
                     /locus_tag="ISB17_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1978857.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(25551..25700)
                     /locus_tag="ISB17_000890"
     CDS             complement(25551..25700)
                     /locus_tag="ISB17_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAP1978858.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            25829..26617
                     /gene="modE"
                     /locus_tag="ISB17_000891"
     CDS             25829..26617
                     /gene="modE"
                     /locus_tag="ISB17_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAP1978859.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26685..28157
                     /gene="modF"
                     /locus_tag="ISB17_000892"
     CDS             26685..28157
                     /gene="modF"
                     /locus_tag="ISB17_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAP1978860.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            28420..29436
                     /gene="galE"
                     /locus_tag="ISB17_000893"
     CDS             28420..29436
                     /gene="galE"
                     /locus_tag="ISB17_000893"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP1978861.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29446..30492
                     /gene="galT"
                     /locus_tag="ISB17_000894"
     CDS             29446..30492
                     /gene="galT"
                     /locus_tag="ISB17_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAP1978862.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30496..31644
                     /gene="galK"
                     /locus_tag="ISB17_000895"
     CDS             30496..31644
                     /gene="galK"
                     /locus_tag="ISB17_000895"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP1978863.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31638..32678
                     /gene="galM"
                     /locus_tag="ISB17_000896"
     CDS             31638..32678
                     /gene="galM"
                     /locus_tag="ISB17_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP1978864.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            32881..33633
                     /gene="gpmA"
                     /locus_tag="ISB17_000897"
     CDS             32881..33633
                     /gene="gpmA"
                     /locus_tag="ISB17_000897"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP1978865.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(33800..34852)
                     /gene="aroG"
                     /locus_tag="ISB17_000898"
     CDS             complement(33800..34852)
                     /gene="aroG"
                     /locus_tag="ISB17_000898"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAP1978866.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            35168..35548
                     /locus_tag="ISB17_000899"
     CDS             35168..35548
                     /locus_tag="ISB17_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978867.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35662..36603
                     /gene="zitB"
                     /locus_tag="ISB17_000900"
     CDS             35662..36603
                     /gene="zitB"
                     /locus_tag="ISB17_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAP1978868.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(36600..37319)
                     /locus_tag="ISB17_000901"
     CDS             complement(36600..37319)
                     /locus_tag="ISB17_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAP1978869.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(37357..38400)
                     /gene="nadA"
                     /locus_tag="ISB17_000902"
     CDS             complement(37357..38400)
                     /gene="nadA"
                     /locus_tag="ISB17_000902"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAP1978870.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38678..38753)
                     /locus_tag="ISB17_000903"
     tRNA            complement(38678..38753)
                     /locus_tag="ISB17_000903"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38718..38720),aa:Lys,seq:ttt)
     gene            complement(38787..38862)
                     /locus_tag="ISB17_000904"
     tRNA            complement(38787..38862)
                     /locus_tag="ISB17_000904"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38827..38829),aa:Lys,seq:ttt)
     gene            complement(38911..38986)
                     /locus_tag="ISB17_000905"
     tRNA            complement(38911..38986)
                     /locus_tag="ISB17_000905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38951..38953),aa:Lys,seq:ttt)
     gene            complement(38990..39065)
                     /locus_tag="ISB17_000906"
     tRNA            complement(38990..39065)
                     /locus_tag="ISB17_000906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39030..39032),aa:Val,seq:tac)
     gene            complement(39117..39192)
                     /locus_tag="ISB17_000907"
     tRNA            complement(39117..39192)
                     /locus_tag="ISB17_000907"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39157..39159),aa:Lys,seq:ttt)
     gene            complement(39195..39270)
                     /locus_tag="ISB17_000908"
     tRNA            complement(39195..39270)
                     /locus_tag="ISB17_000908"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39235..39237),aa:Val,seq:tac)
     gene            complement(39306..39381)
                     /locus_tag="ISB17_000909"
     tRNA            complement(39306..39381)
                     /locus_tag="ISB17_000909"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39346..39348),aa:Lys,seq:ttt)
     gene            complement(39546..40337)
                     /gene="cpoB"
                     /locus_tag="ISB17_000910"
     CDS             complement(39546..40337)
                     /gene="cpoB"
                     /locus_tag="ISB17_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAP1978871.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTSNAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(40347..40868)
                     /gene="pal"
                     /locus_tag="ISB17_000911"
     CDS             complement(40347..40868)
                     /gene="pal"
                     /locus_tag="ISB17_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAP1978872.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(40903..42195)
                     /gene="tolB"
                     /locus_tag="ISB17_000912"
     CDS             complement(40903..42195)
                     /gene="tolB"
                     /locus_tag="ISB17_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAP1978873.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42328..43638)
                     /gene="tolA"
                     /locus_tag="ISB17_000913"
     CDS             complement(42328..43638)
                     /gene="tolA"
                     /locus_tag="ISB17_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAP1978874.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAAD
                     KKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNA
                     SPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLD
                     IKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(43703..44131)
                     /gene="tolR"
                     /locus_tag="ISB17_000914"
     CDS             complement(43703..44131)
                     /gene="tolR"
                     /locus_tag="ISB17_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAP1978875.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44135..44827)
                     /gene="tolQ"
                     /locus_tag="ISB17_000915"
     CDS             complement(44135..44827)
                     /gene="tolQ"
                     /locus_tag="ISB17_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAP1978876.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(44824..45228)
                     /gene="ybgC"
                     /locus_tag="ISB17_000916"
     CDS             complement(44824..45228)
                     /gene="ybgC"
                     /locus_tag="ISB17_000916"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAP1978877.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(45378..45671)
                     /gene="ybgE"
                     /locus_tag="ISB17_000917"
     CDS             complement(45378..45671)
                     /gene="ybgE"
                     /locus_tag="ISB17_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAP1978878.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGFFCPLLADIVLIVGLIFFFF"
     gene            complement(45671..45784)
                     /gene="cydX"
                     /locus_tag="ISB17_000918"
     CDS             complement(45671..45784)
                     /gene="cydX"
                     /locus_tag="ISB17_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAP1978879.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(45799..46938)
                     /gene="cydB"
                     /locus_tag="ISB17_000919"
     CDS             complement(45799..46938)
                     /gene="cydB"
                     /locus_tag="ISB17_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP1978880.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(46954..48522)
                     /gene="cydA"
                     /locus_tag="ISB17_000920"
     CDS             complement(46954..48522)
                     /gene="cydA"
                     /locus_tag="ISB17_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAP1978881.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        11968 a        13017 c        12074 g        11690 t
ORIGIN      
        1 tgcatttatg gatacagaaa acgcttccgc tcgtccataa ggcatatttt atacctggta
       61 cttgttaagt gtcgttttta ataagaaaaa aaatcccgac acatggccgg gatattgatg
      121 atgaaaaaga attaacggcg gttgccgaag atccgcagca acatcaggaa caggttgatg
      181 aagtccagat acaaggttaa cgcgccaaga atggaatatt tgcgcaggtt cgacgtgtcg
      241 cgagtatcaa tctgctcacc catatttttc agtttctggg tgtcatacgc cgtcaatccg
      301 acaaagacaa tcacgccgat gtaggtgact gcccacatca atgcttcgct tttcagccag
      361 aagttaacca gcgatgccag cacaatgccg attaacgcca taaacagcat attgccaaag
      421 ccgcttagat cgcgcttcgt ggtgtaaccg tacaggctca ttgcgccgaa catcccggca
      481 gtaacgacga aagtactggc gatagaagca gcggtataga caatgaaaat actggaaagc
      541 gtaagacccg tcagcgccga ataaagcata aagagcatcg tcgttacacc tgcgctcagc
      601 ttttgaatca tcgctgataa cacaataacc aatgctaatt gcgcgatgat cagaccgatt
      661 aaaaagacac ggttagtaaa caacagctcc atcacggccg cggaattagc cgcataccag
      721 gcaacaaatg ctgtcagcaa caagccaacg gtcatccagc catagacttg cgccatataa
      781 gtttgcaagc cagcccgggg ttgtacgatt gaatcagaac gtgggaatct gtccatgact
      841 ttctcctgaa gatataagga atatcttaag gatactgcaa aatgatgagg ctgtgcatcg
      901 acgcagcgta aacggatgta ctgagcggtg aaattgccgg acgcagcggt gccttatccg
      961 gctaacaata aactaccagc gttttgccgc ctgctgatcg ctctcccgag cttcgaccca
     1021 gcggtcgcct tccggcgtgg cttcgcgctt ccagaacggt gcgcgggttt tgagataatc
     1081 cataataaac tgcccggctt caaacgcact gctgcgatgc gcactggtaa caccgacaaa
     1141 aacaatttca tcgcccggcc ataattcacc aatgcggtga attaccgtga cgcgccccag
     1201 cggccagcgg ttacgcgctt catcaacgat ttctgccagt gctttttctg tcattcccgg
     1261 atagtgttcg agggttaatg ctttgacgct gtcgcccagg ttatggttgc gcaccttacc
     1321 ggtaaaggtg actaccgcac cgtcttcgtc acgctcagcc agccacgggt actcttctcc
     1381 tacgctgaac ggctgcgggc cgacaacaat tttggtttct gccatcttaa cctccggtta
     1441 ccggcgggaa gaaagccact tcgtcgccgt cagtcagcga atggtcaaaa ctcaccagcg
     1501 tctggttgac agcagccagc aatttgccat cttccagcgc cagagcccag cgatcgctct
     1561 gcgcagccag atgctggcgt aacgcttcaa cggttgggaa atccgcagcc acttcggttg
     1621 catcagttcc caccaactcg cgcacctggg cgaaaaaaag aacgttaatc atccgcttcc
     1681 accttaaagt cacccgactt gccgccgctc tttgccagca aacgtaccgg accaatcacc
     1741 atatcttttt gcaccgcttt gcacatgtca taaatggtca gcgccgccac ggaggccgcg
     1801 gttaacgctt ccatttcgac accggttttc ccggtcaggc ggcataaggt ttctatacgt
     1861 acccgattgt gctccggctc ggcctgtaaa ttgacttcaa ctttgctgag catcagcggg
     1921 tgacacagcg ggatcagatc ccaggtgcgt tttgccgcct gaataccggc gatacgcgca
     1981 gtggcaaata cgtcgccttt gtggtggcga ccatcaataa tcatcgccag cgtctcgctg
     2041 cgcatggtga caaaggcttc ggcgcgcgct tcacgcacgg tttccgcttt ggcggagaca
     2101 tcgaccatgt gcgcttcgcc agcggcgttg atatgggtca gttgcgacat acttatttct
     2161 tcaaatgtgg atggaaatta cacggacgcg tacgggcatc cagctgcggc gcaatgatat
     2221 tttcccatgc ggtacggcac gctttggtcg aacccggcat ggcgaaaatc agcgttttgt
     2281 tagcgacgcc cgctaccgca cgagattgca acgtggaagt gccaatctct tcgaacgaca
     2341 acatccggaa cacttcacca aaaccttcaa cttcacggtc gaacaacggc agcagcgctt
     2401 cgggagcctg atctccttcc gtcaggccag taccacccgt aatcaacacc acttgtacat
     2461 cgtcgctggc gatccacgca gagacctgag cgcgaatagc gtagcggttt tctttcacaa
     2521 tggctttatc gacaacgtga tggcccgctt cttgcgccga gtcgcgaaga tagtgaccgg
     2581 aggtatcgtc ttcttcaccg cgacgattag aaaccgtaag aatagcaata cgggtcggga
     2641 taaattcagt gcttacctga ctcatctgat ctctcctttt gacgttttag ccaccaatgt
     2701 acgataagtt ttgcgtaata ccggtgttat tttgatgcag gaaatgggtc tgttttttct
     2761 cccgcagcgc cgctgaaata cgcgcttcca gcgcctgttg ctgggtatcg ttttccagca
     2821 gatcgcgcag gttaacgccg ccttcaccaa acaggcagag atggagttta ccaatggagg
     2881 aaacgcgcag gcggttgcaa gtggcgcaga aatctttttc atacggcatg ataaggccaa
     2941 tctctccggc gtaatccgga tgacaaaaga cttgcgcggg accgtcgctg cgttgacgta
     3001 attggtggat ccagccgcga cgcagtaact cgtcacgcag aacctgacca gagatgtgat
     3061 gcttacggaa gagctcgctg ccctcgcccg tttccatcag ttcgatgaaa cgcagctgga
     3121 tgggacgatg ctggatccag ttcagaaagg tgtcgagctg gtgatgattc acatcacgca
     3181 tcagcacggt attgactttg accttctcaa aaccggcctc aaatgcagca tcgattcctg
     3241 ccatgacctg attgaattta tcctgcccgg taatggcatg aaactggcga gcgtcaaggc
     3301 tatcaacgct gacgttgatg ccagtaagtc cagcatcgcg ccagttcgcc acatcgcgct
     3361 ccagacggta accattggtg gtgaccgcta tctggcgaat agcgtcgttt tctcgcacag
     3421 cggcgatgat atcggtaaag tcgcggcgta aagatggctc accacctgtc agacggactt
     3481 tttcggtgcc cagactggcg aaggcgcgcg taacccggcg aatttcatcg acggtcagga
     3541 agcctttatt ggtgacgccg ctcggcttgt aaccatccgg caggcagtag gtgcaacgaa
     3601 agttacacac atcagtaatc gacaggcgca agtagtaaaa cttacgcgca aatgcatcag
     3661 tcagttgtga agccatgtac acctttccag atacgggagg cgaagtcatt tcttccttcg
     3721 ccctggtggc aatctttacg agcattgtca cggccaaagc accgtatcag ttgacccagg
     3781 tgcagaggct agagtgttta gtggttaagc cgatactagc gtgtaaatga gatttttacc
     3841 atccacactt tcgctatgta atcatgtata tagcgtcatg atcgtgctat ttcgctacag
     3901 aataagggaa aatggcggtt ttactttgat atacatcatc agggaggcat agcgtgcatc
     3961 gtcctttaga ggtagaagaa aatagtttgt atcctcaata ttggcaggtt aattgctgtt
     4021 tcctcgcaat ttgcgctact gtagcgcgga taatctgact ccaggaaact atatgcgcaa
     4081 tcgtacgctg gctgatcttg atcgtgtcgt tgctctcggc ggagggcatg gactgggacg
     4141 cgttctctca tcactttcgt ctttgggttc tcgtttaacg ggtatcgtca ccaccaccga
     4201 taacggtggc tcgacggggc gtattcgccg ctcagaaggc ggcattgcct ggggcgatat
     4261 gcgcaactgc ctcaatcagc tgataactga acccagcgtc gcttccgcaa tgtttgaata
     4321 tcgtttcggc ggtaatggcg aactttccgg gcacaacctt ggaaacctga tgttaaaggc
     4381 actggatcac cttagcgtgc gacctcttga agcgatcaat ctgattcgaa atctgctgaa
     4441 agtggacgca catttgattc cgatgtcgga gcatcctgtt gatctgatgg cgattgacga
     4501 tcaggggcat gaagtttacg gcgaggtcaa tatcgaccag ttaactgcgc cgattcaaga
     4561 gttattgtta acgccgaatg tacccgcaac gcgtgaggcg gttcacgcta tcagtgaagc
     4621 ggatctcatc attattgggc ctggcagttt ttacaccagc ctgatgccaa ttctgctgct
     4681 gaaggaaatc gcacaggcat tacgccgcac gccagcgccg atggtctata tcggcaatct
     4741 ggggcgtgag ttgagtttac ctgcggctaa tctgaagctg gaaagcaagc tggcaattat
     4801 ggagcagtat gttggtaaaa aggtaattga tgcggtcatc gtcgggccaa aagtggatgt
     4861 ctcggcggtg aaagagcgga ttgtgattca ggaagtgctg gaggccagcg atattccgta
     4921 tcgtcatgac cgccagttgt tgcatagcgc gctggaaaag gcattacagg ctttaggtta
     4981 acacgaagcc ggaagaatat tccggcttcg tcatcttgtc acgacgcggc aataaacagc
     5041 tcacgcaact gatgcaactg gtcacgaatt tgcgccgctt cttcgaactc cagattctgc
     5101 gcgtgttgca tcatcaaccc ttccagttca tggatcttct gctgtaacgc tttaggcgac
     5161 atatccatcg gcacattatc cggctcaaca atcgggcgcg attttcctct gcccttcgct
     5221 ttggttttgg caatgttctg ccccagcgcc aggatatcga ccactttctt gttcaagcct
     5281 tgcggcgtaa tgccgtgttc ctcgttgtac ttctgctgtt tctcgcggcg acgttcggtt
     5341 tcgccaatcg ctttcgccat tgatggggtg atcttatcgc cgtagagaat cgctttaccg
     5401 ttaacgttac gtgccgcacg accaatggtc tggatcaacg aacgttcgga acgcaggaag
     5461 ccttctttgt cagcgtcgag gatcgccacc agcgaaacct ccggcatatc cagaccttcg
     5521 cgcagtaagt tgatccctac caatacgtcg aactcaccca gacgcaagtc gcggataatc
     5581 tccatacgtt cgacggtatc gatatctgag tgaagataac gcacgcgctc accgtgttct
     5641 tcgagatatt cagtgagatc ttccgccatc cgcttggtca gagttgtaac cagtacgcgt
     5701 tcgttaattg ccgctcgctg acgaatctcc gatagaagat catccacctg tgtcgctacc
     5761 ggacgcactt caataatcgg gtcgagtaag cctgttggac gcacaacctg atccaccaca
     5821 tcgccaccgg atttttccag ctcgtaatta cccggcgtcg ccgaaacata gatggtttgc
     5881 ggcgctaatg cttcgaactc ttcaaatttc agcgggcggt tatccagcgc tgatggcaag
     5941 cggaagccgt actccaccag tgtctcttta cgcgcccggt caccgcgata catgccgcca
     6001 atttgtggaa tggtgacgtg agattcatcg accaccagca gcccatcagc aggcaggtaa
     6061 tcaaacagcg tcggcggtgg ctcacccggt ccacgaccgg agaggaagcg cgagtagttc
     6121 tcaatccccg aacagtagcc tagctcgttc atcatctcca gatcaaactg ggtacgctgg
     6181 gtcagccgct gctcttccag cagtttgttg ttttccaata gcactttacg tctggcggcc
     6241 agctcttctt tgatctcctc catcgcctgg acgatgcgct cgcgtggcgt gacgtagtgc
     6301 gttttcgggt agatggtaaa acgtggaata gtggaaacaa tctgcccggt cagcgggtca
     6361 aataacgaca atcgttccac ctcctcgtca aacagctcca cgcgaagtgc aatgtcatcc
     6421 gattctgccg ggaagatatc aatcacctcg ccacgaacac ggaaagtacc acgctggaat
     6481 gcttgatcat tacgggcata ttgcagctcc gccagtcggc gcaggatcgc gcgctgatcg
     6541 ataatcatac cgaccgtgag atggagcatc atcttgagat ataaatcagg atcgcccaga
     6601 ccataaatcg cggaaacaga cgccaccaca accacatcac gtcgctccag catcgctttg
     6661 gtggcggata aacgcatctg ttcgatatgt tcgtttaccg aggcatcttt ctcaataaag
     6721 gtgtcggaac tcggtacata ggcctccggc tgatagtagt cgtagtagga gacgaaatat
     6781 tccaccgcgt tttctgggaa gaattctttc atttcgccat acagctgggc cgccagcgtt
     6841 ttgttgggcg caagtaccat ggttgggcgc tgaaggtcag caatgacatt ggcaatggtg
     6901 aaggttttcc cggagccagt cacgccaagt aacgtctggt gcgccaggcc atcttccagc
     6961 ccctcttcga gacgtcgaat cgcctccggc tgatcgccag aaggtttaaa agcggaattc
     7021 agtttgaacg gtttactcat gagtcgctac ctgaaggagt tgggcgggca ggtatgtaat
     7081 tttactcgtc gtacttaatt atgccaacaa attatactgg ataaaaaaac agttcatcac
     7141 cataatattt ctgttacagc gtaaactccg ctgtcaatca tgaacaagat ttactctgcg
     7201 gcgagataaa actccggcct taccgggtta tccccaaagc aacggctttt ttaacatttg
     7261 tcaagatgat tgatgacagt tttatggcaa gagatgcctg ttcagtgact gccattgcaa
     7321 ttatctaacc tgttaaaaaa taaaagatat ttctttgagc tgtctttaac gccaattcgc
     7381 gtgcaagccg cgtattctct cgcttgcctc gtgttttcta actctaatac acatggttat
     7441 ccacaggaat agtggataac tgtctgcagc ccctatcccc cgccgcctgg gcaattccca
     7501 cattcaccgc atgatgcgga ggtaaaattt ttttcgtgat ttttttagaa tttaattggt
     7561 taaattgcag tcaatcggag acacgatctc gctcgcaatt taaccaatta caggatgact
     7621 acattaaggc aagattgatg tactttccgg ttgccgcatt ttctggattt tctgcaagcc
     7681 agggaatttc gcccagcagc ggcgcgggaa tcatgcgggt gagcgtggtc atatattcag
     7741 cgtgacgttt tcccggaggc gtgacatcgt tcgccaccca ccccgccaga gtcagtccgg
     7801 cgtgtagtat tgcctgcgca gtcaacatcg cgtgattaat acagccgagt ttcacgccca
     7861 caaccagtat cactggcagt tgttcctgtg ttacccaatc cgcaaaagtg aaagtgtcag
     7921 aaagcggcgt aaaccagccg ccagcacctt ccactaacac ccagtcagcc tgttgttcaa
     7981 gcgcgcgtaa tccggcgctc attaccaatg cttctatcgg cctgccctct tgcgcgctga
     8041 tgatatgcgg cgaggtgggt tctgcgaagg tgtaaggatt taccgttgcg taatccagct
     8101 gcaggctact gttgcgctgt aacgccagcg cgtcactatt acgtaagcct ttcggggtct
     8161 tttcgctgcc agaggccacc ggtttgtaac ctgccgtccg gtagcctgct gcctttgcgg
     8221 cttgtaaaag cgcgcagctg gcgacagttt tccccacttc ggtatccgtt ccggtgacaa
     8281 aataacattt actcacgagc aatcactccc aaaaaaagat gatacgtcag aggatatcgc
     8341 ccctgttgtt gcggccaggc cagttgcaat cgctgcaact gcgaacgcgt taatattcgc
     8401 gggtcacgcc cttcatgaag atgcgtggca ccgatgcctt tcagcgaacg catggcactg
     8461 agcgcatcat caaaccacag cgtgatgggc tgaatataat gttgataatg cacggcgttt
     8521 agcgactgtt cgatttcatc tggcggtaaa aagcgattag catgcggacg ctcgtccacc
     8581 gcctgccacg cctgatgcag ttcgggtaac gatccctgca ccagcgtggt aaacgcgacc
     8641 acgcctccgg ggcgcaccac ccgatacagc tcgcggagtg ccgtggataa attaccgcac
     8701 cactgcaccg cgagattgct ccatgcaaga tcgaacgtcg cagtcgctaa cggcagggat
     8761 tcgatatctc ccgccagata atggtctgcg gcatccttct ggcgtgcctg aacaagcatt
     8821 ggcggagaga gatctaacgc agtcacctgc gcgtgacgct ctcgccagcg gcggctcatc
     8881 cagcctggtc cacaacccgc atccagtacg cgggtgtatt tacgctgtgg gagcattgcc
     8941 agtaaggcgt cagcactctg gcgctgtaga tcggcgtgtt gctcatagtg tgcggctgcc
     9001 cgtccaaatg ccgcagcaat ggcttgttta ttaaccgttg ccatgcagca cctccagcag
     9061 acggtcgata tcctgcattt catgcgctgc ggttagcgtt aagcgcagtc gcgcagtacc
     9121 agcgggtacg gttggcgggc gaattgccgt gacccaacag ccttgctggc gcagtttttc
     9181 tgccagttgt aacgcacggc tgttatcacc gacgatcaat ggctggatgg cgctccatga
     9241 atcagcaagc gtaaacggca aatcctgcac tccggcacga aaacgcgtta tgagtgccgc
     9301 cagtttttcg cgccgtgcat ccccctcatc actgcgaatg actgccagcg acgcacgtaa
     9361 tgcctgcgcc tgagcgggcg gcatactggt gctgtagata agatggcgag cgaattgcag
     9421 cagataatcc gccaccgtat tggagcaaag caccgctgcc ccgctgacgc caaatccttt
     9481 gccaaacgtc accaccagca attctggttt taccttttgc agccagcagc tgccacgccc
     9541 ctgctccccg ataacgcccg tgccgtgggc gtcatcgacc atcaaccagc catcgtgctg
     9601 ttgcgttacc tgctggattt cctccagtgg cgcactatcg ccgtccatgc tgaacacgcc
     9661 ttctgtcact accagttgct gccccggaca gggggaagca agcagtcgcg ccagatgagt
     9721 gacatcgtta tgagcaaaac ggcgaagcgg cgacgggctt aaacttgcag cctccagcaa
     9781 tgaggcatgg ctaagccggt cggcaacaat acggtcctct ttcgccatca tcgcggcaat
     9841 gactgcctga ttagcagcaa aaccagagat aaacagcagt gcccgcgaat agcccagcca
     9901 ctcggccagt tcttcttcca gcgcctgatg cgccacgcta taaccgctga cgtgaccgga
     9961 gccgccgcta ccgatgccaa attgctccgc accctgcttc caggcacgga taatttgcgg
    10021 atgatggctt aaacctaaat aatcgttact ggaaaagttc agatactggc gatcatccac
    10081 caccagccag cgtccggctc cttgcgccac cggataacgg cgacgcaggg catcggcagc
    10141 acgccgcgca tcgagcgccg cgttgatttt ctcctgccag ctcatagtgc tgccgcgttg
    10201 taatattcgt cagtgtccgg ggtcatcagc gcctgctcca gacgctgctg ttgttcgtta
    10261 tcgcccgcca gcacggcagt ttgctgcgga tttagcccca gtttgcggaa cagttgcagg
    10321 tctttatctt cttccggatt cggcgtggtc agcagtttgc aaccgtagaa aatcgagttt
    10381 gcgccagcca taaagcacat cgcctgagtc tgttcgttca tctgctcgcg tccggcagaa
    10441 aggcgtacgt aagaggtcgg catcatgatc cgcgcaactg caatggtgcg aataaaatca
    10501 aaggcatcga catcatcgtt atccgccagc ggcgtgcctt tcaccttcac cagcatgttg
    10561 attggtacgc tttccggcgg cgttggcagg tttgccagtt gcagcaataa tccggcgcga
    10621 tcttttactg tttcgcctaa gcccacaatg ccgcctgaac agactttgat cccggcgtcg
    10681 cgcacttttt ccagcgtatc gaggcgttcc tgataggtac gcgtggtgat gatattgccg
    10741 taaaactccg gcgaggtgtc gaggttgtgg ttgtagtaat ccagcccggc attcgccagg
    10801 cgctgcgcct gagattcact caacgtgccc agcgtcatac acgcctccag ccccatcgct
    10861 tttaccccct gcaccatttg ttccaggtac ggcatatcgc gttcgtgggg attcttccac
    10921 gccgcgccca tacagaaacg cgtcgatccc gccgctttcg ctttgcgcgc cgactccagc
    10981 acctgttcaa cttccatcaa ccgctcggct tccagcccgg ttttgtagcg cgagctttgc
    11041 gggcaatatt tgcaatcttc cggacaagct ccggtcttaa tcgacagcaa agtgctgacc
    11101 tgcacctgac gaggatcgaa atgctgacga tgcacctgct gcgcttcaaa cagcagatcc
    11161 agcaacggtt tttcaaataa ttctgtgact tgcgacaatg tccagcgtgg gcggtgagcc
    11221 atggggcttc tccaaaacgt gttttttgtt gttaattcgg tgtagactcg taaacctaaa
    11281 tcttttcaat ttggtttaca agtcgattat gacaacggac gatcttgcct ttgaccaacg
    11341 ccatatctgg cacccataca catccatgac ctccccgctg ccggtttatc cggtggcaag
    11401 cgccgaaggt tgcgagctaa ttttgtctga cggcagacgc ctggttgacg gtatgtcgtc
    11461 ctggtgggcg gcgatccacg gttacaatca cccgcagctt aatgcggcga tgaagtcgca
    11521 aattgatgct atgtcgcatg tgatgtttgg cggtatcacc catgcgcccg ccattgagct
    11581 gtgccgcaaa ttggtggcaa tgacgccgca accactggag tgcgtttttc tcgcggactc
    11641 cggctccgta gcggtagaag tggcgatgaa aatggcgttg cagtactggc aggccaaagg
    11701 cgaagcgcgc cagcgttttc taaccttccg caatggttat catggcgata cctttggcgc
    11761 gatgtcggtg tgcgatccgg acaactcaat gcacaacctg tggaaaggct atctgccaga
    11821 aaacctgttt gctcccgccc cgcaaagccg catggatggc gaatgggatg agcgcgatat
    11881 ggtgggcttt gcccgcctga tggcggcgca tcgtcatgaa atcgcggcgg tgatcattga
    11941 accgattgtc cagggcgcag gcgggatgcg catgtaccat ccggaatggt taaaacgaat
    12001 ccgcaaaatg tgcgatcgcg aaggtatctt gctgattgcc gacgagatcg ccaccggatt
    12061 tggtcgtacc ggcaaactgt ttgcctgtga acatgcagaa atcgcgccgg atattttgtg
    12121 tctcggtaaa gccttaaccg gcggcacaat gaccctttcc gccacgctca ccacgcgcga
    12181 ggttgcggaa accatcagta acggcgaagc cggctgcttt atgcatgggc caacttttat
    12241 gggcaatccg ctggcctgcg cagcagcaaa cgccagcctg gcgattctcg aatctggcga
    12301 ctggcagcat caggtggcgg ctattgaagc gcagctacgc gagcaacttg cccccgcctg
    12361 tgacgccgaa atggttgccg atgtgcgcgt actgggggca atcggcgtgg tcgaaacctc
    12421 tcgtccggtg aatatggcag cgctgcaaaa attctttgtc gaacagggtg tctggatccg
    12481 gccttttggc aaactgattt acctgatgcc gccctatatt attctcccgc aacagttgca
    12541 gcgtctgacc gcagcggtta atcgtgcggt gcaggatgaa acattttttt gccaataacg
    12601 ggaagtcagc gtgagggttt ctggctacac tttctgcaaa caagaaagga gggctcatga
    12661 aactcatcag taatgattta cgcgatggcg ataagctgcc gcatcgtcat gtctttaacg
    12721 gcatgggtta cgatggcgat aatatttcac cgcatctggc gtgggatgat gttcctgtgg
    12781 gaacgaaaag ttttgttgtc acctgctacg acccggatgc gccaactggc tccggctggt
    12841 ggcactgggt agttgttaat ttacccgcgg atacccgcgt attaccgcaa gggtttggat
    12901 ctggtctggt agcaatgcca gacggcgttt tgcagacgcg taccgacttt ggtaaaaccg
    12961 gatacgatgg cgcagcgccg ccgaaaggcg aaacccatcg ctacattttt accgttcacg
    13021 cgctggatgt agaacgtatt gatgtcgatg aaggtgccag cggcgcgatg gtcgggttta
    13081 acgttcattt tcactctctg gcgagtgcct cgattactgc gatgtttagt taatcactct
    13141 gccagatggc gcaatgccat ctggtatcac ttaaaggtat taaaaacaac tttttgtctt
    13201 tttaccttcc cgtttcgctc aagttagtat aaaaaagcag gcttcaacgg attcattttt
    13261 ctatttcata gcccggagca acctgtgaac acattttcag tttcccgtct ggcgctggca
    13321 ttggcttttg gcgtgacgct gaccgcctgt agctcaacac cgcccgatca acgtccttct
    13381 gatcaaaccg cgcctggtac ctcttctcgc ccgattctgt cggcaaaaga agcgcagaat
    13441 ttcgatgctc aacactattt tgcatccctg acaccaggtg cggcagcgtg gaatccttcc
    13501 ccgattaccc tgcctgcgca acctgacttt gttgtcggcc cggcgggtac tcaaggtgta
    13561 acgcatacca cgattcaggc ggcggtagat gcggcaatta tcaagcgtac caacaagcgc
    13621 cagtatattg ccgtgatgcc tggtgagtat cagggaacgg tgtatgtccc tgccgctccg
    13681 ggtggaatta ctctgtacgg tacaggtgaa aaaccgattg atgtgaagat tgggctttcc
    13741 cttgatggtg gcatgagccc tgccgactgg cgtcacgacg tcaacccgcg cggcaaatat
    13801 atgccaggta aaccggcgtg gtatatgtac gatagctgcc agagtaaacg cagcgacagt
    13861 atcggtgttc tctgctctgc ggtcttctgg tcacaaaaca atggcctgca actgcaaaac
    13921 ctgaccatcg aaaacacgct gggcgatagc gtagatgcgg gtaaccatcc ggcggtggca
    13981 ctgcgtactg atggcgacaa agtgcagatc aataacgtca acattctcgg tcgtcagaac
    14041 accttctttg tcaccaacag cggtgtgcag aaccgtctgg aaacgaatcg ccagccgcgt
    14101 acgctggtga ccaacagcta tattgaaggg gatgtggata tcgtttctgg tcgcggcgca
    14161 gtggtgttcg ataacaccga attccgcgtg gtgaactccc gtacccagca agaagcgtat
    14221 gtgtttgcac cggctacgct gtccaacatt tactacggtt tcctcgccgt aaacagccgt
    14281 ttcaatgctt ccggtgatgg cgtggcgcaa ctgggccgct cgctggatgt tgatgccaat
    14341 accaacggtc aggtagtgat ccgtgatagc gccatcaacg aaggttttaa cacagccaaa
    14401 ccgtgggctg atgcggtgat ctctaatcgc ccgtttgcgg gtaacaccgg cagcgttgat
    14461 gataacgacg aagtacaacg caatctgaat gacactaact acaaccgcat gtgggaatac
    14521 aataaccgcg gcgtgggtag caaagtggtt gcagaggcga agaagtagga gctgttaact
    14581 atgtgccgga tgcggcgtaa acgccttatc cggcctacag ttcgatacga tttgtcggtc
    14641 ggataagatg cgccagcatc gcatccgaca ataagtgccg gatgcgacct acattcacat
    14701 ggcgcttttt acatctgacg gtttttattg aagttaatca aactacccgc cttgataatc
    14761 tcgcgttctt cggcagtcag actttccata tagagcgtga tttccgttac cggtgcatct
    14821 tcatggatca cataaccttt aaacgtcgtt ccaggattat ccagcgccgc tttaatgcca
    14881 gggatgtaaa tgtaatcccc cacttcaaag gttggcgctt ccgccatctg cagcggtaac
    14941 atcccccagt tgatgacgtt agaacgatag cgtttagtcg cgtactcctc ggcaatattc
    15001 gccagaccgc caatcacacg ctgacaactc gccgcctgtt cacgcgcaga accatcgcct
    15061 ggtttcaccg catagaccat gctgccaatt tcagtttgca gcggatcaac atgttcctga
    15121 ccagcaatct gcttaatgcg cgcaaacacc tctgccatct cgctgacatt ccccgccaga
    15181 cgctgatttt ccagctcagc agtcgcttta cttctgccaa cataacctgg atcgcggcgt
    15241 gacagggtaa actccgccag accaatcgga tttgaacgat aagaagaggt ttcaccggaa
    15301 ggaatcagtt cgtcggtggt ggtcacttcg tcgaggatct tcgagcacac tttgaggacg
    15361 atattgtcag tcagcgcacc caattccggc cagtctttaa tgttcggtcc gtaaatcagc
    15421 ggttgctggg tcgccccttt cacaaagcct tgatagacgc gatttttata cggcgttaca
    15481 tcgaaggcgt actccggcac gttgtcccag caatcgagtt cgctggcaga ggttaaatag
    15541 ccaccgtttg ccgcagtcgc agcgatagaa cgagcgtcca tcaacgccac cgctgacatc
    15601 tgcccattag ctggcttaga gccttcgcgg ttcgggaagt tacgcgtggt gtggcgaata
    15661 ctcaaaccgt tgttgattgg cgtatcgccc gcgccaaagc atgggccgca gaacgcggtt
    15721 ctgatgattg cgcctgcgcc aatcaaatct gcgaccacac cttttttggc gagatccata
    15781 aacaccggct gtgatgacgg gtaaaccgct aacgagaagg tgtcattgcc gcaggattga
    15841 ccgcgcagcg cattcgccgc cgcgatgacg ttttcatagt taccgccaga acagcccgcg
    15901 ataatcccct gctgcacttt caggcgacca ttttccactt tatccagcag cgagagtttg
    15961 gctttaccgt gcgccacgcg ttcggactca atttcaatct cacgcagaat gtcggtcagg
    16021 ttctggttca gcgtgtcgat ttcatacacg ttgctcgggt ggaatggcag cgcaatcatt
    16081 ggtttgatgg cgcttaaatc aacgctgatg cagccatcgt agtacgccat tggttgaggg
    16141 ttaagttggc agtaatcctg gccgcgaccg tgcagcgcca gccagttatg gacttcttca
    16201 tcggtttgcc agacagaact taaacaggtc gtttcagtgg tcatcacgtc aacgctgtta
    16261 cggaaatcgg tagagagcgc tgcaacaccg ggacctacga actccatcac tttgtttttg
    16321 acgtaaccgt ttttgaacac ggcaccgatg atagccagcg ccacatcctg cggccccaca
    16381 tacggcgcag gttttccggt cagatgcacc gcaaccacgc ccggatagtc gatatcccag
    16441 gtgtcattaa gcagttgttt taccagctcg ccgccgccct caccgactgc cattgtccct
    16501 aatgcgccgt aacgggtgtg gctgtctgat ccgaggatca ttttgccgcc gccagccatc
    16561 atttcacgca tatattgatg gatgaccgca atatgcggtg gcacaaaaat accgccataa
    16621 cgctgggccg ccgataaacc aaaaacatgg tcatcaccgt taatggtgcc gccgacggcg
    16681 cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc cataccggac
    16741 gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa tgaatcaaac
    16801 ttaattttaa gtttatccat atttccggac gtattatgag aagagagaat agaccaggca
    16861 atagtgcctt ttttggcttc ttctttttta atttcgccgg tgaaatgttc ttcggcaatt
    16921 atttcgttat tactggcgag aaacacgcct ttttcagata acttgatcat caaatgctcc
    16981 agatatctgc gcaccggaaa gtgcgcagaa agataataaa tggatatact ctaaataatt
    17041 cgagttgcag gaaggcgaca agcgagtgaa tccccagtca cttacataag taagtgactg
    17101 gggtgagcga acgcagacgc agcacatgca acttgaagta tgacgagtat atttacagca
    17161 tgttccagcc gatcagcata ttccaccacc acacgccgag ggtgatatgc accaggaagg
    17221 ttaatatcgt cagtaccgca ccgaccaacc accaggattt aatatcgttg taacccacgc
    17281 caaagatgac cggacctgcc gcgccgccat agtgagtaac catgccgcca taggagttag
    17341 agaacaacag tgccagcgcg gttaacatta acggtgcgcc ggagacattc gccagcatgg
    17401 caaataccgg taacatggca acgatatagg cactaccaga agcgaagaaa taacgcacga
    17461 tgatgctgag gaaaataata acgaagaaag caacgttccc gtgaccatca aatgccaggt
    17521 tatttttaaa gacttcagct aaccattcga agaatttaac tttcgataat aaggagctta
    17581 agccgataat accgccgtac cagattaagg tattccagcc gcctttattt ttaaccacgt
    17641 cttcccaggt aacgataccc agcagcagca tagttgccat aacaacgatt gccacggtgg
    17701 attcatcaac ccccagagac ttactgaaaa tccagcccag cagcgccagc acaaagacac
    17761 cgagcagcat tttttcgcgg attttcatcg gtcccagttc ggcaaggccc gctttagcga
    17821 tggttttgtt atccaccttc ttaatttctg gtggatacat ggtgtaaatc accagcgggg
    17881 tgaccagcag cataatgatg cccggcaatc cggcggctaa cgcccatcca ccccagctaa
    17941 tttgcaggtg cagaatgtcg ttgatcattt tcagcgccag aatgttcccc gccattgcgg
    18001 taaagaacat atagctggtg gttttggtga ccatgtaaat ggacatcatc aggtaatgtc
    18061 caacacgacg cggacttttt tccggttctg atcccaaagc cactgccacg ctgttgatga
    18121 tcggtaacac gatgccgccc gcacgcgcgg tgttagacgg tgttgccgga gccagtacca
    18181 gatcgagaaa taccgtaacg taacccagac ccagggtagt gctaccaatt ttaccaatca
    18241 gcagataggc aatacgttta cctaagcctg tggttacaaa cgcggcgctt aaagtaaacg
    18301 cagaaaacac cagccaggtg gtaccggaag agtaaccgct taatacagcg gtggttttaa
    18361 attccccacc tgataagtta cccaccacta ccatcgatgc ggcgacggca attaacagta
    18421 cgacaggttc cgggaaaggc ttgataacca gccccacaat ggccgccagg taaataccaa
    18481 aaagcaccca cgccagttcg cttaatcctg ccggagctgg cataaagcca ataatacatg
    18541 ggattgctaa tatcagaatt agcttccata acgatttctt attcatttaa tatcctgttc
    18601 tttctgtatg actcatacag aattccttta ttgattgtca ttatcacgac aacaaaattt
    18661 ttcagggaag ataaacttca ccggaaaata tttttctggt cgtccgaata acagatgcgc
    18721 gtaacgtcgt ggcatcctga ccttcattac ttaaatgaac gtcgagcgca ccactggggt
    18781 gttcaatatt gatattgccg tatcctacag aagggacgat ttgtcgggtg acggtacctt
    18841 ccaatgcaca actactggaa atagcaatag caccggttat cgccaaagcg cgatggcaag
    18901 aatgcggcat aaaataacgc acattaattg ccccgccttt ctgcgctgga gaaataagta
    18961 caggtttagg gataaccata ttactgacat cgcctaaacc cattgcttta cccgcttgta
    19021 gacggataga ttcaatgcgg gctaataatg ctttgtcggc atccagctct gccggtaatt
    19081 catagcctgt ttttcccaga tattcagcag gaataatgac aactggcatc gccatatcga
    19141 tacaggtcac cgggacatcg tcaaaataat caatctgatt atccgtcggg aagacttttc
    19201 cggttttcgt tccggcggca ttcaggaaag tgagcgcaac cggtgcggca gtacccggta
    19261 cgccgtcaat tctggcgcta ccctcgtact cgacaacacc atttggcgtt tgcacatcag
    19321 cttcgatgaa cgtacccgta ttgacgttgc ggatacgtac gcgggtaact ggcgaagtcg
    19381 ctgcaatcaa accattttca atggcgaatg ccccaacgcc agacagcata ttgccgcagt
    19441 taggcgtggt atcgacacgt tgctcatgga cgataacttg agcaaacaga taatcgacat
    19501 ctgcacgcgg atcgctggag cggctaataa tggcgacttt actggtcagc ggattaccgc
    19561 cgccaatacc gtcaatttcc agatcgttac cggaacccat aattgccatc aatattttat
    19621 cgcgctgcgt ttgatcttcg ggtagatgtt ccgctaacag gaacgcgccc ctcgaggttc
    19681 cacctcgcat catcacgcag ggtatttttt tcataataat gccctcaatt taaaaacata
    19741 acttattgcg atatggttac attaagggca aagcatctct aatgacttta gctcaacgat
    19801 taataacatt ttttcattaa tttaagttgg ctaaatgcag ttaaaatttt taactgccag
    19861 tcacccaaaa tcgtcgcttc cattttccgg agtaataatg aagcatgaat tatcaagtat
    19921 gaaggcattt gtcatactgg cagagtccag ttcatttaac aatgctgcta aattactcaa
    19981 tattacgcaa cctgcattaa cgcgaagaat aaaaaagatg gaggaagatt tacacattca
    20041 actatttgag cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa ggttgctccc
    20101 ggaggcgcgg gaattaataa aaaaattcga tgaaacgctt tttagtattc gcgacatgaa
    20161 tgcttatcat cgaggtatgg tgacgttagc gtgtattccg accgcggtat tttatttttt
    20221 accattggca atcggtaaat ttaatgagct gtatcccaat attaaagtgc ggattctgga
    20281 acaaggcacg aataattgca tggaatcggt gctgtgtaac gaatcagact ttggcataaa
    20341 catgaacaac gtcacaaatt catccattga ttttacccca ctggtcaacg agccgttcgt
    20401 gctggcctgt cggcgtgacc atccattggc caaaaagcag cttgtagaat ggcaggagtt
    20461 agtgggttac aaaatgattg gcgtacgctc ctccagcggc aaccgactgt tgatagaaca
    20521 acagctagcc gacaagccct ggaagctgga ctggttttac gaagtgcgtc atctttcgac
    20581 gtcattaggg ctggtggagg cgggactggg gatttcggcg cttcctggtc tggcaatgcc
    20641 ccatgcgccc tattcttcga ttattggtat cccactggtg gaacccgtta tacgtcggac
    20701 attggggatt attcgccgca aagatgccgt gctttctccg gcagctgaac gcttttttgc
    20761 cttgctgatt aatttgtgga ctgacgataa agacaatttg tggaccaata tcgtcgaacg
    20821 ccagagacat gcgttacagg agataggtta atacaccggc gcaggagaga ctgcgccggt
    20881 taaatcagcg gttagtgcgc gttgaccact acccacattg gcccctgccc aaccgcatag
    20941 cggccttttt catgcagtag cccctgctcg ccaacaattt cgtataccga gatgtggtga
    21001 gatttttgcc cggcggcaat cagatacttg ccgctgtgat caacattgaa gccgcgcggc
    21061 tgggtttccg ttggctggaa gccttcttta ctcaacacgc tgccatcttc cgaaacgctg
    21121 aaaacggtaa tcagactggc ggtacggtcg caggcgtata aatgacgacc atccggggtg
    21181 atatgaatat cagccgccca acgggtgtcg gagaagtttt ccggcatcat atccagcgtc
    21241 tggacacatt cgatattgcc gtgcggatct ttcagttccc agacatccac tgagctgttt
    21301 aactcattga cgcaatacgc atattgttcg tttggatgga ataccatatg acgcgggccg
    21361 gccccttcaa cggtagtcac ttccgcaggg tcctgcgcca ccagatgacc atcatcgctg
    21421 accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa cccacaacgt acggttgtcc
    21481 ggtgagatat tggcggaatg gcaaccgtcc agcccctcga ccacatcgac gacgcccact
    21541 ggcaggccat cttccagacg cgttacgctc acgttacccg cattgtaaga accgacaaag
    21601 acaaactgcc cctggtgatc ggtggaaata tgcgtcgggc tacccggcag cgcagactct
    21661 gcagcaaagg tcagcgcgcc atcatccggt gcaatacgat acgccagaac acgaaactcc
    21721 ggacggacac caacgtagag gtaacgtttg tccgggctga ccaccatcgg ctgcacctgc
    21781 cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt catgattcag gttccagacg
    21841 tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa tgctcctttg
    21901 catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta
    21961 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
    22021 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc
    22081 ttattgaccc cgaaaaagac cctgcttcca tcatcgatag aagccctggc ccgcgctcgc
    22141 gaagcaggct atcgattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt
    22201 tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat
    22261 tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc
    22321 attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtggatga tgcgatggtc
    22381 tatgagcacc cgaccggaca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg
    22441 gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacggcgca acaagttaac
    22501 gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag
    22561 catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt
    22621 gcacgcggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggctgg
    22681 tcgatggaaa acgtcgtggc gttcggcgat aacttcaatg atattagtat gctggaagcc
    22741 gctggtacag gcgtggcgat gggcaacgcc gatgacgcag taaaagcgcg cgccaacatt
    22801 gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa
    22861 tcaggcggtt atcgacacac ttttaatttg cgcatacagc cacaggccag gtttgatcgc
    22921 cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttacagc cgacttccag
    22981 ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt
    23041 acgaatgctg gtttgctgcg gcggttgtaa aaccagtgag acatcggaag cctgaatgcg
    23101 gatgcgtagc gcagcttgca gcggttcgtc cagcttattg acccacaaat gctgatcgcc
    23161 cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agtactgtca ctttcagaat
    23221 gctactttgt tgctctttcg gtagccacgg attcatcacg ctactacccc agacttcttc
    23281 cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg
    23341 gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt cccgcgtcag
    23401 acgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgatg ccagcggttc
    23461 atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca cgcgctgttt
    23521 ttcgcctccg gacaggctgc ctggtaaacg atcaagcaac ggttcaatgc ctaaaagcgc
    23581 caccagctta tcgaactgat tgaccatgct tttcgccatg ccgtagcgca aattgccacg
    23641 cactttataa tgcggaaaca gccgcgcatc ctgaaacaca tagcctacgc gacgcttttc
    23701 tggcgtcagg cagatacctt tatcggcgtc atttagtacc cgaccattga ggacaatccg
    23761 ccctttttgc ggacgtgtca gcccactgat ggcgttaatc agcgaggttt ttccggcacc
    23821 agagacacca aaaatagcgg tgatgccatt ggcgggcagc gtttcattaa tcgtcaggca
    23881 atggttgccc aacgtctggg aaaaattcag ttccagcatg attagcgccc cgcccgctca
    23941 cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa
    24001 ataatacaca gtctcgccgc gccactttcc ccgccagggg tctggatcag ggtatacatg
    24061 gcagaaggaa tggtccgcgt ttcaccagga atgttcgaca caaaggtgat ggttgcgcca
    24121 aattcaccga gcgaacgagc gaatgccagt accgtaccaa caataattcc cggtaaggtc
    24181 agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt tcttgcggcc
    24241 tgctccagtt tgatatcaac accttccagc gccagacgaa tcgcccgcac catcagcgga
    24301 aaagacatga cagcggcagc gagaaccgca ccacgccagc taaacgcgaa agaaagacca
    24361 aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataatagg
    24421 taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct
    24481 ttgcccggaa acgtgcaacg taccagtaac caggcaaaaa agatcccaaa cggcaggcta
    24541 aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg
    24601 gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgca gcctgcggcc
    24661 ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa
    24721 ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt
    24781 tgctggcaac tgcgtcagaa ccgtaaacaa tacccagcgg cgcttcattg cgttcgacca
    24841 acgccagcgc gccacgaaca tcttccgccg gagccagttt cggggaaagc gtatcccatg
    24901 cgcccagttt ttgcagtgct tctttcgcat aaatgccagc agggacatgt tccggatcac
    24961 caaccgcgag gcgaccgcca ttcagcagcg aagtccagtt ggttttgctg tcgatggtga
    25021 aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct
    25081 ggcgcgaagc cgtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg
    25141 cagaaataaa cagatccgca ggcgcacccg cttcaatctg acgggccaaa gtagatgacg
    25201 aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta gcaatgtcct
    25261 gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg
    25321 cattgccagc aacagcgaaa gagagtgccg ccccggtaaa caagttcaac catttacgag
    25381 ccattggtaa ccccttaatc taacgttatg taggcgtcaa tataacggca agttcggaat
    25441 tttcccagca gttattgcta acctttgtga ggtagatgag aaaaaatatc ggcagaaaaa
    25501 gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa tcaatgattt
    25561 tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg
    25621 taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga
    25681 ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct
    25741 aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc
    25801 acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct
    25861 ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc
    25921 cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc
    25981 cattaacgag atgaatcagt taagtgagca tattctggtt gagcgcgcaa caggcggtaa
    26041 aggtggaggc ggcgcagtac tgacccgcta tggtcagcga ctgattcaac tctatgactt
    26101 actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg ccctgccgct
    26161 gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg
    26221 gttcggtacc atcaccgctc gcgatcatga tgacgttcaa cagcatgttg atgtcttact
    26281 ggctgatgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cgcgtctggg
    26341 gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gtattactca
    26401 ggacgaggcg gtcgcgcaaa acgctgacaa ccaattaccg ggtattatta gtcatattga
    26461 gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc
    26521 cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca cggcctactt
    26581 taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa
    26641 acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa
    26701 ttttgcaagg cacgtttcgt cttggcgaca caaaaacgct gcaattgcct cggctaacgt
    26761 taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa tcggcgctgg
    26821 cccgcgcgct gtcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc
    26881 acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcagcgga
    26941 ataacaccga tatgcttagt cctggcgaag atgacaccgg gcgtaccacg gctgagatca
    27001 ttcaggatga agtaaaggat acttcgcgtt gcatgcaact ggcgcagcag ttcggtatta
    27061 ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc
    27121 tgtgtcaggc gctgatgtcg gagcctgact tgttgatcct tgatgaaccg tttgatggcc
    27181 tggatgttgc ctcacgtcag catctggctg agctactcgc ctcgttacat cagtccggta
    27241 ttactctggt actggtgctc aatcgcttcg atgagatccc ggaatttgtc cagtttgctg
    27301 gcgtgctggc ggattgcacg ttagcggaaa ctggcgctaa agaagaactg ctccaacaag
    27361 cactcgttgc gcaactggcg catagcgaac tgcttgaagg tgtgcaaatg ccggagccgg
    27421 atgaaccttc agcacgtcac gccttacccg ccaacgaacc gcgcattgtg ctgaacaatg
    27481 gcgtggtttc ttataacgaa cgccccatcc ttaataacct tagctggcag gtgaatccag
    27541 gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga
    27601 ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga cgacgtcgcg
    27661 gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc agtagtttgc
    27721 atctggatta ccgggtcagc actactgtgc gtaatgtgat tctttctggc tattttgatt
    27781 cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag cagtggctgg
    27841 atattctcgg cattgataaa cgcacggctg acgctccttt ccatagtctt tcctggggac
    27901 agcagcgtct ggcgctgatt gtccgcgcac tggtgaaaca tccgacgttg cttattctcg
    27961 atgaaccact acaggggctt gatccgctca atcgccagct tatccgccgt tttgttgatg
    28021 tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc
    28081 ctgcctgtat tacccatcgt cttgagttcg tgccggacgg tggactctat cgctatgtgc
    28141 tgacaaaaat aaattgagtc ggtaatgctg accgtgccgg aggcggcgta aacgtgttat
    28201 ccggccaacg gtttgacgca tccaggcatg aaaccgcgtc ttttttttca gataaaaagc
    28261 gcaatcattc ataaagccgc tgttttataa tcacttaatc gcgcataaaa aacggctaaa
    28321 ttcctgtgta aacgattcca ctaatttatt ccatgtcaca cttttcgcat ctttgttatg
    28381 ctatggttat ttcataccat aagcctaatg gagcgaatta tgagagttct ggttaccggt
    28441 ggtagcggtt acattggaag tcatacctgt gtgcaattac tgcaaaacgg tcatgatgtc
    28501 atcattcttg ataacctctg taacagtaag cgcagcgtac tgcctgttat cgagcgttta
    28561 ggcggcaaac atccaacgtt tgttgaaggc gatatccgta acgaagcgtt gatgaccgag
    28621 atcctgcacg atcacgctat cgacaccgtg atccacttcg ccgggctgaa agccgttggc
    28681 gaatcggtac aaaaaccgct tgaatattac gacaacaatg tcaacggtac tctgcgcctg
    28741 attagcgcca tgcgcgccgc taacgtcaaa aactttatct ttagctcctc cgccaccgtt
    28801 tatggcgatc agcccaaaat tccatatgtt gaaagcttcc cgaccggcac accgcaaagt
    28861 ccttacggca aaagcaagct gatggtggaa cagatcctta ccgatctgca aaaagcccaa
    28921 ccggactgga gcattgccct gctgcgctac ttcaacccgg ttggcgcgca tccgtcgggt
    28981 gatatgggcg aagatccgca aggcattccg aataacctga tgccgtacat tgcccaggtt
    29041 gctgtaggcc gtcgcgactc actggcgatt tttggtaacg attatccgac cgaagacggc
    29101 actggcgtgc gcgattacat ccacgtaatg gatctggcag atggtcacgt cgtggcgatg
    29161 gaaaaactgg cgaacaagcc aggcgtacac atctacaacc tcggcgcagg cgtaggcagc
    29221 agcgtgctgg acgtagttaa tgccttcagt aaagcctgcg gcaaaccggt taactatcat
    29281 tttgcaccac gtcgcgcggg cgaccttccg gcctactggg cggacgccag caaagccgac
    29341 cgtgaactga actggcgcgt aacgcgcaca ctcgatgaaa tggcgcagga cacctggcac
    29401 tggcagtcac gccatccaca gggatatccc gattaaggaa cgaccatgac gcaatttaat
    29461 cccgttgatc atccacatcg ccgctacaac ccgctcaccg ggcaatggat tctggtttca
    29521 ccgcaccgcg ctaagcgccc ctggcagggg gcgcaggaaa cgccagccaa acaggtgtta
    29581 cctgcgcacg atccagattg cttcctctgc gcaggtaatg tgcgggtgac aggcgataaa
    29641 aaccccgatt acaccgggac ttacgttttc actaatgact ttgcggcctt gatgtctgac
    29701 acgccagatg cgccagaaag caacgatcct ttgatgcgtt gccagagcgc gcgcggtacc
    29761 agccgggtga tctgcttttc accggatcac agtaaaacgc tgccagaact gagcgttgcg
    29821 gcattgacgg aaatcgtcaa aacctggcag gagcaaaccg cagaactggg gaaaacatac
    29881 ccgtgggtgc aggtctttga aaacaaaggc gcggcgatgg gctgctctaa cccgcatccg
    29941 cacggacaga tttgggcaaa tagcttcctg cctaacgaag ctgagcgcga agaccgtctg
    30001 caaaaagaat atttcgccgg gcagaaatca ccaatgctgg tggattatgt tcagcgcgag
    30061 ctggcagacg gtagccgtac cgttgtcgaa accgaacact ggttagccgt cgtgccttac
    30121 tgggctgcct ggccgttcga aacgctactg ctgcccaaag cccacgtttt acggatcacc
    30181 gatttgaccg acgcccagcg cagcgatttg gctctggcgt tgaaaaagct gaccagtcgt
    30241 tatgacaacc tcttccagtg ctccttcccc tactctatgg gctggcacgg cgcgccattt
    30301 aatggcgaag agaatcaaca ctggcagctg cacgcgcact tttatccgcc tctgttgcgc
    30361 tctgccaccg tacgtaaatt tatggttggt tatgaaatgc tggcagaaac ccagcgagac
    30421 ctgaccgcag aacaggccgc agagcgtttg cgcgcagtca gcgatatcca ttttcgcgaa
    30481 tccggagtgt aagaaatgag tctgaaagaa aaaacacaat ctctgtttgc caacgcattt
    30541 ggctaccctg ccactcacac cattcaggcg cctggccgcg tgaatttgat tggtgaacac
    30601 accgactaca acgacggttt cgttctgccc tgcgcgattg attatcaaac cgtgatcagc
    30661 tgtgcgccac gcgatgaccg taaagttcgc gtaatggcag ccgattatga aaatcagctc
    30721 gacgagtttt ccctcgatgc gcccattgtc gcgcatgaaa actatcaatg ggcgaactac
    30781 gttcgtggcg tggtgaaaca tctgcaactg cgtaacaaca gcttcggcgg tgtggacatg
    30841 gtgatcagcg gcaatgtgcc gcagggtgca gggttaagtt cttccgcttc actggaagtc
    30901 gcggtcggaa ccgtattgca gcagctttat catctgccgc tggacggcgc acaaatcgcg
    30961 cttaacggtc aggaagcaga aaaccagttt gtaggctgta actgtggaat catggatcaa
    31021 ctgatttccg cgctcggcaa gaaagatcat gccttgctga tcgattgccg ctcactgggg
    31081 accaaagctg tttccatgcc gaaaggcgtg gctgtcgtca tcatcaacag taacttcaaa
    31141 cgtaccctgg ttggcagcga atacaacacc cgtcgtgaac agtgcgaaac cggtgcgcgt
    31201 ttcttccagc agccagctct gcgcgatgtc accattgaag agttcaacgc tgttgcacat
    31261 gaactggacc cgatcgtcgc gaaacgcgtg cgtcacatcc tgactgaaaa cgcccgcacc
    31321 gttgaagctg ccagcgcgct ggagcaaggc gacctgaaac gtatgggcga gttgatggcg
    31381 gagtctcatg cctctatgcg cgatgatttc gaaatcaccg taccgcaaat tgacactctg
    31441 gtagaaatcg tcaaagctgt gattggcgac aaaggtggcg tacgcatgac cggcggcgga
    31501 tttggtggct gtatcgtcgc gttgatcccg gaagaactgg tgcctgccgt acagcaagct
    31561 gtcgctgaac aatatgaagc aaaaacaggt attaaagaga ctttttacgt ttgtaaacca
    31621 tcacaaggag caggacagtg ctgaacgaaa ctcccgcact ggcacccgat ggtcagccgt
    31681 accgactgtt aactttgcgt aacaacgcag ggatggtagt cacgctgatg gactggggtg
    31741 cgactttact ttccgcccgt attccgcttt ccgatggcag cgtccgcgag gcgctgcttg
    31801 gctgcgccag cccggaatgc tatcaggatc aggccgcatt tctgggggcc tctattggtc
    31861 gttatgccaa ccgcatcgcc aatagccgtt atacctttga cggtgaaacc gtgacgcttt
    31921 caccaagtca gggcgtcaac caattgcatg gcggcccgga agggttcgac aaacgtcgct
    31981 ggcagattgt gaaccagaac gatcgtcagg tgctgtttgc cctgagttca gatgatggcg
    32041 atcagggctt cccgggtaat ctcggcgcga cggtgcaata tcgtctgacc gacgataacc
    32101 gtatctccat tacttatcgc gccacagttg ataaaccttg cccggtgaat atgactaatc
    32161 acgtctattt caatcttgac ggcgagcagt ctgacgtgcg caatcataag ttgcagattc
    32221 tggcggaaga atatctgccg gttgatgaag gcggcattcc gcacgacggc ctgaaatctg
    32281 tcgccggaac ctcttttgat ttccgcaacg ccaaaatcat cgccagtgag tttcttgccg
    32341 acgacgatca gcgcaaagtg aaaggttacg atcacgcgtt cttgttacag gccaaaggcg
    32401 atggcaagaa agtggcggcg catgtctggt cagcagatga aaaattgcag ctgaaggtct
    32461 acaccaccgc tccggctctg caattctact ccggcaactt cctcggcggc acaccgtcgc
    32521 ggggaaccga accttacgct gactggcaag ggctggctct ggaaagcgaa tttctgccgg
    32581 acagcccgaa ccaccctgaa tggccgcaac cggactgctt cctgcgtcct ggcgaagagt
    32641 attccagcct gacggaatat cagtttattg ctcagtaatg ttaagccctc gtcatgaggg
    32701 ctttatcctc atattgttca aatcaccagc aaacaccgac atatttgcaa ctcaatattc
    32761 acaacaacct tacactgcga cactattttc gctatggtta tgcgtaagca ttgctgttgc
    32821 ttcgtcacgg caatataatg agaattatta tcattaaaag atgatttgag gagtaagtat
    32881 atggctgtaa ctaagctggt tctggttcgt catggcgaaa gtcagtggaa caaagaaaac
    32941 cgtttcaccg gttggtacga cgtggatctg tctgagaaag gcgtaagcga agcaaaagca
    33001 gcaggtaagc tgctgaaaga ggaaggttac agctttgact ttgcttacac ctctgtgctg
    33061 aaacgcgcta ttcataccct gtggaatgtg ctggacgaac tggatcaggc atggctgccc
    33121 gttgagaaat cctggaaact gaacgaacgt cactacggtg cattgcaggg tctgaacaaa
    33181 gcggaaaccg ctgaaaagta tggcgacgag caggtgaaac agtggcgtcg tggttttgcg
    33241 gtgactccgc cggaactgac taaagatgat gagcgttatc cgggtcacga tccgcgttac
    33301 gcgaaactga gcgagaaaga gctgccactg acggaaagcc tggcgctgac cattgaccgc
    33361 gtgatccctt actggaatga aaccattctg ccgcgtatga agagcggtga gcgcgtcatc
    33421 atcgccgcac acggtaactc gttacgtgcg ctggtgaaat atctcgataa catgagcgaa
    33481 gaagagattc ttgagctgaa tatcccgacc ggcgtgccgc tggtttatga gtttgacgag
    33541 aacttcaaac cgctgaaacg ctattatctg ggtaatgctg acgagatcgc ggcgaaagca
    33601 gcggcggttg ctaaccaggg taaagcgaag taaacgtcat tcgcttaaaa atgagaaagc
    33661 cgactgcaaa atgagtcggc ttttttattt ttcgttgcta ccaatggagc gcattgcctg
    33721 atgcgacgct gcgcgtttta tcaggcctac ggtgattaat cggatacgcc actctgacag
    33781 cgcatccgac aattaaactt tacccgcgac gcgcttttac tgcattcgcc agttgacgta
    33841 acagagcatc ggtatcatcc cagccaatgc aggcatcggt gatgctctta ccgtaggcca
    33901 gcggttcccc gctctcgagg ctctgattgc cttccaccag atggctttcc accatcacgc
    33961 caataatggc cttttcgcca ccggcaatct gctggcaaac gtcagcacaa acatccatct
    34021 gctttttgaa ttgtttagac gagttagcat ggctgaaatc gatcatcacc tgtgctggca
    34081 ggcctgcttt gttcagccct tctttcactt cagcaacgtg cttcgcgctg tagttaggct
    34141 ctttaccgcc gcgcagaatg atatggcaat cgccgttacc gctggtattc acaatcgccg
    34201 aatgacccca tttggtcact gaaaggaagc agtgcggcgc accggcggca ttaatggcat
    34261 cgatagccac tttaatcgta ccatcagtgc catttttgaa gcctaccggg caagaaagcc
    34321 ctgaagccag ttcgcggtgc acctgcgatt ccgtagtacg tgcgccaatt gcgccccagc
    34381 tcatcaggtc agcgagatat tgtggggtga tcatatcgag aaactcaccc gccgctggca
    34441 gaccgctgtc gttaatatca agcaacaatt tacgggcaat acgcagaccg tcgttgatct
    34501 ggaagctgtt atccatatgc ggatcgttaa tcagcccttt ccagcccacc gtggtacgcg
    34561 gcttttcaaa atagacgcgc attacgattt ccagctcatc tttcagctct tcacgcagcg
    34621 ccagcaagcg agtggcatac tctttagccg cgacaggatc atgaattgag catgggccaa
    34681 tcaccaccaa caggcgatca tcattacctt tcaggatctt atggatcgct tttcgggcat
    34741 gggcgaccgt attcgcggca ttttcagtag cggggaattt ttccagcaat gcgacaggag
    34801 gaagtaactc tttgatttct ttgatgcgta aatcgtcgtt ctgataattc atgtctgttc
    34861 cagtgttgcc atacttatct tagtgaatgc aatacttcca atctatatct tccgtcagaa
    34921 tgtgtaaacg gggttttaca ctatgaacgg attaatcctg gaattagcaa gaaaaacgcc
    34981 agattgtcgc gaaaaacgag atctctccta caatttctaa ctgtaactcc tttgtttgtt
    35041 aattatttca agattctctg ctgggtatct ttgcctggct gaaatcttaa accattgcct
    35101 tatattcacc tgcaaatgca ctgttggaag aggttatccg acataacgac cataacagga
    35161 gcatcctatg aaaatgacaa aactggccac actatttttg actgccactc taagccttgc
    35221 cagcggtgcc gcactggctg ccgatagcgg agcgcaaact aataacggcc aggcaaacgc
    35281 cgccgctgat gcgggccagg tagcccctga tgcccgtgaa aatgtcgcgc caaataacgt
    35341 cgacaataac ggggtaaata ccggttctgg cggcacaatg ctgcatccgg atggttcttc
    35401 aatgaacaat gacggcatga ccaaagatga agagcacaag aacaccatgt gcaaagatgg
    35461 tcgctgcccg gatatcaata aaaaagtcca aaccggtgat ggcatcaaca atgatgtcga
    35521 taccaaaacc gacggtacca cgcagtaacc ctgcgaaata gccaataatg ggagagcgta
    35581 gtctctccct tttaattttt cccttctaaa agcagtatga tgtcataaag ttaagtgaga
    35641 ataattaagg gatgactcgt catggcgcac tcacactcac acacttcctc acacctgcca
    35701 gaagataata atgctcgtcg cttgttgtat gctttcggcg tgactgccgg gtttatgctc
    35761 gtagaagtca ttggtggttt tctttccggt tctctggcat tgctggccga tgcgggtcat
    35821 atgttgaccg atactgccgc cctgcttttc gccctactcg cagtgcaatt ttcccgtcgt
    35881 cctcccacca ttcgccacac tttcggttgg ctaagattga ccacgctcgc ggcgtttgtg
    35941 aatgccatcg ccctggtggt gattactatt ttgattgtct gggaggcgat agaacgcttc
    36001 cgcactccgc gtccggtcga gggcggcatg atgatggcaa ttgccgtggc tgggctgctg
    36061 gcaaatatac tttctttctg gttacttcat cacggcagcg aagagaaaaa cctcaacgtg
    36121 cgagcagcgg cactgcatgt gctcggggac ctgctgggtt cggttggggc tattattgcc
    36181 gcgttgatta ttatctggac cggctggaca cctgctgacc ccattctctc aatactggtg
    36241 tcgcttctgg ttctgcgtag cgcgtggcga ctgttgaaag atagtgtgaa tgaattactt
    36301 gaaggtgcac cggtatcgct ggatatcgct gaactgaagc gtcgcttgtg ccgggaaatc
    36361 ccggaagtcc gcaatgtgca ccatgtgcat gtatggatgg taggcgagaa gccggtgatg
    36421 acgctgcatg tgcaggtgat cccgccacac gatcacgatg ccttgttgga tcagatccaa
    36481 cactacctga tggatcacta tcagattgag cacgccacca ttcagatgga atatcaacct
    36541 tgtcatgggc cagactgcca cctgaataga ggggtgtctg gccattcaca tcatcatcat
    36601 taatgggaca gcgcacgtga acctctttcg cgtgcgcttt tgatccacat ccggctgccg
    36661 ttgagggcaa taaaggtcag gatgatgtac tccagagaca tggcgtaaac gccctgaagt
    36721 gcaaaaataa cgacgctaat cacgttaata atcacccaca acagccagtt ttccacatac
    36781 ttacgtgtca tcagaatcat tgccacgata gataacacca tcatgcatga atcccagaac
    36841 gggaaagcgt ccggctgtag ttcaggcatc gcaacctgta atcctaatgc ttgcatgatc
    36901 atgactgcca cgcgggtcaa aaatgcaaac acaggattga taaataccgt cattagacca
    36961 atcgaaacaa cgcaaaccgc cagccagctt agcgccttcg gcaatggcaa ccagcgaatt
    37021 ttcaactccg cctcgttctg actggtttgt cgcgaccacg cataccagcc gtaaatattc
    37081 gcggcaaaga aaaacacctg taataacagg ctggcataca gctgaatctg aaagaaaata
    37141 atgccaaaca aggtgacgtt gatcaggcca aagaagtagt tactgatctt ctccagactg
    37201 gcaaggccaa tacacagcaa cccggcgatc gtgcctaccg cttcgatcca tgagagatca
    37261 taaccgcctg cccctattgg tatatgtacc aggatattct gcacactaaa aaaatccatc
    37321 gttcccccaa agcgtaatgt gacgccttat tatcaattat ccacgtagtg tagccgcaaa
    37381 atccagcata cgattgagcg gcaccagcgc cctctctcgc agcctttcat caacataaac
    37441 ctcgtgattg cttccttcca gttctaatgc ctctgcgatg gcctgaaggc cattcatggc
    37501 catccacgga caatgcgcgc agctgcgaca ggttgcgccc tcacccgcgg ttggtgcttc
    37561 cagtaactct ttatctggca ccgcctgctg cattttgtag aaaatgcccc gatcggttgc
    37621 cacgataagc cgctgatgtt gcaatgtttt cgcagcagcg atcagttgac tggtggaacc
    37681 gaccgcatcc gccatctcta caatagcttg cggtgattct ggatgcacca gtatggcagc
    37741 atccgggtat tcttcttgca agcgggttaa cgcctgagtc ttaaactcat catgcacaat
    37801 gcaggcaccc tgccagcata aaatgtctgc gcccgtctgt ttttgcacgt aacgccccag
    37861 atgtttgtca ggtgcccaga tgattttttc acccaaacta tccagatgat caataagttc
    37921 gacggcaatg cttgaagtta ccacccaatc tgcgcgcgct tttaccgcag cagaagtgtt
    37981 ggcgtagacg acgacagtac gatcgggatg ggcatcgcaa aatgcgttaa attcttcaac
    38041 agggcagccg agatccagtg aacattcagc ctgaagtgtc ggcatcagaa ttgttttttc
    38101 cggactgaga attttggcgg tttctcccat aaatctcacc ccagcgacta gcaacgtaga
    38161 agcgggatgc tttgcaccga agcgcgccat ttccagagaa tcagaaatac agccaccggt
    38221 ttcttctgcc agttgttgaa tttcgggatc ggtatagtag tgggcaacca tcaccgcatt
    38281 acgttctttt agtagacgtt ttatcttctc gcggtaattc gctttttcat caatgcttaa
    38341 cggcgtcggc ttcgggggga aaggataaat cgccgtgtct ggatcaaaca tcacgctcat
    38401 cttaccatct cgttctacag gcttaacgtt aaaaccgact taagcgtgat attcgctatt
    38461 atcggcgtaa tttgttttat atactaaaca agatagtcga atggtgggga aaagtcactc
    38521 gaattacgta tgagatgccg aatgccacac tttgcgtctt attcctccga cgtagagttg
    38581 tttacgcagc aaacgcggag tatgtcatca gggtcaagaa ttgaaattga gagatgcctg
    38641 aatagcaaaa aaacgccttt agggcgcttt tttgcattgg tgggtcgtgc aggattcgaa
    38701 cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga ccccttgcgg
    38761 gattatgctg ctttaatcat tcaggatggt gggtcgtgca ggattcgaac ctgcgaccaa
    38821 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccatacgggt gttacctgaa
    38881 agatttctac tcggaaccaa tgtttaaaat tggtgggtcg tgcaggattc gaacctgcga
    38941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgagt ggtgggtgat
    39001 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    39061 cacccgatac tgcgctggat actacaaact acttccaccg aacccaccgt aatcagtggt
    39121 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
    39181 agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg
    39241 gaggtgctct cccagctgag ctaatcaccc gatactacgc tggatactac aactaactat
    39301 gttagtggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    39361 taccaactga gctaacgacc cactttttcg ttgctttcgg tttgtttgat atcccgtggc
    39421 aacggcggca tatattactg atttcagact tgagcgcaac aaaaatttcg atgtagatca
    39481 ctcaactgct tatgattcgc acgacacgac cagaaataat gcgacttctg gtcgtgtgtt
    39541 atgcattaca tcgcgttcag acgtttttgc gcctgtttag cgccatcagt accagggtat
    39601 ttactgataa cctgctggta tacggctttc gcttttgcgg tgtcaccttt gtcctgcatg
    39661 atgacgccga ctttaaacat cgcatctgca gccttcggtg acttcggata gtttttcact
    39721 accgaagcaa aataatacgc cgcatcatct tttttaccct tgttgtagtt taactgaccc
    39781 agccaataat tggcgtttgg caggtaagtt gaatcagggt aatttttgat gaaattctga
    39841 aatgccacca ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat agctgcattg
    39901 taatccgtgt ttgcatcacc gcttttcacc ggcgcgccag cattcgaagt accagcatca
    39961 gctgtcggcg tcgttgatgc caccacaccg ctttgatcgc cgctggttga ttgcgccgct
    40021 gcaccaccgc tgctgaggct gtcgatctgc aacaggatct gcttttgccg ctccacgacc
    40081 tgattcagtt gatactgatt ttcctgaatc tgaccacgca gggaatcaat atcggattga
    40141 ttatcagaga gttgttgctg gagttgggtt aaaagctggc tgtgagcatt agaaatacgc
    40201 tcaagttgag tgacgcggtc ttcgaccgag cctgagccga cactactgat tggtgcctga
    40261 gcaaaagcgg cccagggggc cgctatacca accagtaacg acagactcaa tagttgatgt
    40321 ctgaagttac tgctcatgca attctcttag taaaccagta ccgcacgacg gtttttggca
    40381 tacgccgctt cgtcatgacc cagtactgca ggtttttctt taccgtaaga aacgatggag
    40441 atctggtcag cagaaacgcc tttaccctgc agatacatct taacagcgtt cgcacgacgt
    40501 tcacccaggg agatgttgta ttccggagta ccacgttcgt ccgcgtgacc ttctacggtg
    40561 actttgtaag acgggttgct acgcaggaag tttgcgtgtg catccagcat ttgagcgaag
    40621 tcagaacgga tatcgtactt gtccagatcg aagtaaacga tgttgttctg ctgcagctgt
    40681 tgcatttgca gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc gttcgcatcc
    40741 ataccagtgc cggcacccag catgccttcg ctgccgtcat tgctggcgtt cttgttggaa
    40801 gaacatgccg caattgccat aacaggcaga gcaatcatca gccctttcag cactttgttc
    40861 agttgcattt caatgattcc tttactattc aattaattat tatcacagat acggcgacca
    40921 ggcagggaat ttgacctgtc catcagttgc cggaagacgc gctttgaaac gcccatctgt
    40981 agaaaccaaa ttcagcacgg atcccatccc ctgagaagag ctgtagatta ccatagtgcc
    41041 gttaggtgcc agacttggcg tttcatccag gaacgtggac gacagaactt gtacgcctcc
    41101 cgttgccaga tcttgtttgg caatgtgctg ctgcccaccg ttggagctga ccattaccat
    41161 aaatttaccg tcgctgctga catccgcatc ctggttctgc gaaccttccc aggtaatacg
    41221 ttgtggcgca ccgccgttga tattcacttt ataaacctgc ggacgaccgg cctggtcaga
    41281 agtaaatgcc aggttctggc tgtccgggaa ccaggtcggt tcggtattgt tactgcgacc
    41341 atcagttacc tggcggatct gaccagaagc caaatccatt acgtacaggt tcaggctacc
    41401 ggttttcgac aaggcgaatg ccagtttgct gccgtctggc gagaatgcag gtgcaccgtt
    41461 gtgacgcggg aatgaagcca cctgacgtac agcgccattt gccagcgtct gaataaccag
    41521 cgcggaacga ccgctttcga aggtcacata agccagttta gaaccgtctg gtgaccacgc
    41581 cggagacatc agcggttgcg gtgaacggtg aacgacaaac tggttgtaac cgtcatagtc
    41641 agatacgcgc agttcatacg ggaactgacc gccgttagtc tgaacaacgt aggcaatacg
    41701 ggtacggaac gcacctttaa taccggtcag tttttcaaat acttcgtcac tggcggtatg
    41761 accagcataa cgcagccact gcttgttcac tttgtacgag ttctgagcaa gtacagtacc
    41821 cggtgcgccg ccagtgtcaa caagttgata agcaacattg taagaaccat ccggattcgg
    41881 agtgacctga ccgacaacca cagcgtcaat gcccagcgcg gaccatgcag ctggttgtac
    41941 ttcctgcgca ctacccggct gctgtggcag acgagcacga tctaacggat taaatttacc
    42001 gctgttacgc aagtcagcag caacgatgcc gccaatatct tcaggtgccg caccaggccc
    42061 cgcccactgg aaaggaacaa caccaatagg acgaccggaa tctacaccgc tgtcgatcac
    42121 aatgcggact tcagcatgca gaactgatgc ccacagtatg agaaaaccaa atgctactcg
    42181 taatgcctgc ttcatcatat ctcccttatc tggaccgatg gcccacgata atttagcaga
    42241 atgttaacaa actaaaatac acaaaactac cagaaccccg tggcaaccgg tgcctgatgt
    42301 tgaccgtcgg aacagtcaac atcgcgatta cggtttgaag tccaatggcg cgtttttgaa
    42361 cacttcatat actgcctggc ttggtggttt cgggatcttc gcaagtttag ctgctgccaa
    42421 cgcagcttga caaagtgcgg gatcgccacc ttcaggtttg atatccagta acataccatc
    42481 gggtgccagt tttatgcgca gcgtacaggt tttgcctgca taggacgatg cgtcatagaa
    42541 cttactttcg atagcagatt taatctgccc ggcatagtta ttgatatcgg cccctgatgc
    42601 gccattgttt ttagtattac cactcccggc aggcgaagca ttgttccctt tcgcccctcc
    42661 ccccgttttc ggtgcattct taccagagct tagctcaccg aaaatatcat ctgcctcagc
    42721 ggcagctttt gctgcagcgg ctttttcagc tgctgctttt gccgctgccg cttttttgtc
    42781 tgcggcggct ttttctgccg ctgctttctt atcagctgct gccttttcag cagccgcttt
    42841 ttctgctgct tttttgtcgg ctgccgcttt ctctgctgcc gctttcttat cagctgcagc
    42901 cttttcagca gccgcttttt ctgctgcttt tttgtcggct gccgctttct ctgctgccgc
    42961 tttcttctca gcttctgcct tggctttttc agcagcttcg gttgccgctt tctttcttgc
    43021 ttcagctgca gctgcttctg ccgcttcagc tttcttcttc agcgccgcag cagcagcctc
    43081 ggcttttttc tgcgcttcga ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc
    43141 gtctgccgcc gctttcttcg ctgcttcttc cgcagcttta gcatccgctt cggccttcgc
    43201 tttagcatct gccgccgctt tcgctgccgc ctcttccgct tgcttctgct ttaactcggc
    43261 ctgttttgcg gcttcttcag cctgcttttt ctgctcctga gccgctaacc gctctttctc
    43321 aagttgcttc aggcgttcct gttcagccgc ttgtttctca cgcagttctt cagcagcctg
    43381 ctgctctttc atcttgcgct gctcatcaga acgcttcgcg cttgattcct ggctttgcat
    43441 gcgtttgtac tgctcaacta ccgcacctga atcaaccatg acagcgtcga tggacgaacc
    43501 accgccgcct ccggctgaag cttctatatt ctcatcgaac gaactccaga tcagcgccgc
    43561 aaataagatg acatgcagca ctgctgaaat aattatcgcc cgcttgagct tgtcgttttg
    43621 ttcggttgcc tttgacactc tcggtttcca aaaactgttc gcctgttacc cgctctcttt
    43681 caagcaaggg aaacgcagat gtttagatag gctgcgtcat taaaccaacc gatttcacac
    43741 ccgcactatg taacaagttc agtgctttaa ttatttcatc gtaaggcaca tcttttgcgc
    43801 caccgatcag aaagaccgtt ttcgggttgg ccttgaaacg gctggacact tccgccacca
    43861 cctgctctgg tggtaaacgc tccaggcgat ctttctcaac caccacggtg tactgaccaa
    43921 taccagacac ttcaacaatc actggcggat tatcgttact gctcaccgcc tgtgattcag
    43981 tagcgtctgg cagatcgacc tccacgctct gggtgatgat gggcgctgtc gccataaaga
    44041 tcagcaacag caccagcagt acgtccagca acggtacaat gttgatttcg gacttgagat
    44101 cgcgacgacc tcgtccacgc gctctggcca tggcttaccc cttgttgctc tcgctaacgg
    44161 taaacgcctg gcggtgcaga atcgcggtaa actcttccat aaagttgtcg taattcagtt
    44221 ccagtttgtt tacgcgctgg ttgagacggt tataagccat taccgccgga attgcggcga
    44281 acaaaccaat tgccgtcgca atcaacgctt ctgcgatacc gggcgcaacc atttgcagtg
    44341 ttgcttgttt taccgccccg agggcgataa aggcgtgcat gatcccccag accgtaccaa
    44401 acagaccaat atacgggctg atggagccaa ccgtaccgag gaacggaatg tgcgtttcca
    44461 gattttcaag ttcacgattc atggaaatac gcatcgcacg cgacgcccct tccactacgg
    44521 cttccggcgc atggctattg gcacgatgca ggcgcacaaa ctctttgaag ccgctgtaaa
    44581 agatttgttc cgaaccgctc agattatccc gtttcccctg gctctcttga tagaggcgag
    44641 agagttcgat tccagaccag aatttatctt caaacgcttc ggcttcgcgc gccgctgcgt
    44701 taagaatacg ggtccgctgg ataataatgg cccaggatgc gattgaaaaa ccaatcaaaa
    44761 tcaacatgat aagtttaacc agaaggctag ccttcaggaa caaatcaagg atattcatgt
    44821 cagtcactgc ttaaactccg cgacaataga cttgggaagc gcacgaggct tcattttgag
    44881 tgggtcaacg caaacaacca gaacctctgc ttcattcaac aaagtattct cggcgttgac
    44941 aatacgttgc gtgaaaacca aagaggtgcc acgcattgat gttatttcag tctgtatttc
    45001 gagcatatcg tcgagccgcg caggtgcgta atattccacc gtcattttac gtaccacaaa
    45061 ggcaacgcgt tcagccatca gcgcctgttg actgaaatga tgatgacgca gcatctctgt
    45121 gcgtgctctt tcataaaaag cgacgtaact ggcgtggtac accacaccac cggcatcggt
    45181 atcttcatag tagacgcgaa ccggccatcg aaacagcgtt gtattcactt tacatcccgg
    45241 taatgcaaca aaagttagag cttttaaacg ctgctactat acgcgcgcga ttgaggtttg
    45301 ggaatgaggg caagttaagg gagcgtaaaa atttatgggc ctgactcggc ccataaattt
    45361 ttaaagagaa ttctgattta aaagaagaag aaaatcagcc cgacaatgag aacaatatcg
    45421 gcaagcaatg ggcaaaaaaa cccttgccag agaaccttct gcggacgaaa gcccacgccg
    45481 tgaatcacac cagcacagac ggcccacata agcagtaaac catgccagat ttccagttca
    45541 ctggtcttcg cggcgaaacg tgatgggtcc caaaacatac atcctgctaa cagaagtgcc
    45601 atcacgaagg aaagcgcccg cagggggcgc ttgtccatta ccgcatacaa ggttgcgata
    45661 atcttactca tcagatgtct tcttgaccgg ctttgcctga ttcgacgtgc tcaagcgcca
    45721 gcgcggtgat taccccaaac gaacaggcaa gaagcgttcc cagaatccat gcgaaatacc
    45781 acatttttag ctccttactt agtacagaga gtgggtgtta cgttcaatat cttctttggt
    45841 gatacgaccg aacattttcc agtaacacca ggcggtgtag agcagaatga tcggtaccag
    45901 aaccaccgca acccaggtca tgacgttaag cgtcagctgg ctggaagttg catcccacat
    45961 tgtcagactt gcgttcatca tggtgctgga cggcatcaca aacgggaaca ttgcgatacc
    46021 ggcagtcagg atgatgcagg ccagcgtcag ggaggagaaa acaaacgccc acgcggcttt
    46081 atccatacgt gcagtcagga tggtcagcag cggcagaacc acacccagtg ccggaatagc
    46141 ccacagaatt ggcgtgttgt tgaagttaac cagccatgcg ccagcttcac gaaccacttc
    46201 tttattcagc gggttagagg ctgcgtaatg gtccattgtc gatttcacga cataaccatc
    46261 gataccgtac atcacccata cgccagccag tgcgaagcag accagcgtca ccagtgccgc
    46321 cacctgagcc gttgcacggg tacgcaggtg cagttcgccc acggtacgca tttgcagata
    46381 ggttgcgccc tgagtaatga tcatccctac gctcaccaca cctgccagca ggccgaacgg
    46441 gttaagcagc tggaagaagt taccggtgta gtacagacgc agatattcat caacgttgaa
    46501 tggtacgccc tgcaacaggt taccgaacgc tacaccaatc accagcggcg gaacgaagct
    46561 accaatgaag atgccccagt cccacatgtt acgccagcgg gtttcttcaa tcttggagcg
    46621 gtagtcaaaa ccgaccggac ggaagaacaa agacgccagt acgaggatca tcgccacata
    46681 gaagccggag aacgcagcgg catagaccat cggccaggca gcaaacagtg caccgcctgc
    46741 ggtgatcagc caaacctggt taccgtccca gtgtggtgcg atggagttaa tcataattcg
    46801 acgctcggtg tcgttacgac cgaggaaacg ggtgagcatg cccaccccca tgtcgaaacc
    46861 gtcagtgact gcaaaaccaa tcagcagaac gccaaccagc agccaccaga taaaacgcaa
    46921 tacttcataa tcgatcattt gacgactcct gtcttagcgt gccggctgag tagtcgtgga
    46981 agactgctca aagtgatagc gaccggtttt caggctgctt gggccgaggc gtgcaaactt
    47041 gaacattaag aacaattctg ccaccaggaa cagggtatac aggccgcaaa tcagcaccat
    47101 tgagaagatg agatcgcctg cggtcagtga cgagttcgcc acagctgtcg gcagcacttc
    47161 accgatagcc cacggttggc ggccatattc agccacgaac cagcccgctt ctacagcaat
    47221 ccacggcagc ggaataccgt acagcgcggc gcgcagcagc cattttttct cgccaatgcg
    47281 gttgcggatg acactccaga aagagagcgc aatgattgcc agcagcagga agccacacgc
    47341 caccatgata cggaacgcga agtacagcgg cgctacacgc ggaatggagt ctttggttgc
    47401 ctgttgaatc tgagcttcag tcgcatcagc cacgtttggc gtatagcgtt tcagcagcag
    47461 accgtaaccg agatctttct tcatgctatt gaactggtca cgaacggcct ggtcagtaga
    47521 accagaacgc agttgttcga gcagagagta cgccttcatc ccgttacgaa tgcgttcttc
    47581 atgctgcacc atcagttctt tcagaccgat aactggagta tccacggaac gcgttgcaat
    47641 gatgcccagc gcgtaaggga tctggatcgc aaatttgttc gtctcctctt cctgatcagg
    47701 aatgccgaac agagtaaagg cagcaggtgc aggttgcgtt tcccactcgg cttcaatagc
    47761 agccagtttg gttttctgca cgtcgcccat ttcgtagccg gattcatcac ccagaacaat
    47821 aacagacaga acggcagcca taccgaagct ggcagcgata gcaaaggagc gtttagcgaa
    47881 ggcgaagtca cgacctttca gcatatacca tgcgctgata ccgaggatga acatcgcgcc
    47941 agtcacataa ccagaagcta cggtgtgaac gaatttcacc tgagcaaccg ggttaagcac
    48001 cagctcggag aagctcacca tctccatacg catagtttca aagttgaaat cggacgcgat
    48061 tgggttttgc atccagccgt ttgcaaccag aatccacagt gcggacaggt ttgaaccgag
    48121 cgccaccagc caggtgacac acatatgctg aactttaccc agacgatccc aaccgaagaa
    48181 gaacagacct acaaaggtgg attcgaggaa gaaggccatc agaccttcga ttgccagcgg
    48241 cgcaccgaag atatccccta catagtggga atagtaagac cagttagtcc cgaactggaa
    48301 ctccatggtc agaccggtag ccacacccag agcaaagttg ataccaaaca acttgcccca
    48361 gaacttggtc atatctttat aaatctgttt gccggaaagg acgtagaccg tttccataat
    48421 ggccagcagg aacgccatac cgagcgtcag tggcacaaaa aggaagtggt acatcgcggt
    48481 caaggcaaac tgtaagcgcg acagttcgac tatatctaac atcatgactc cttgctcatc
    48541 gcatgaagac tccgagagtg aaccccgtta gaaagggtca cacgcatgcc ccaatacaaa
    48601 attatttgct cgcctctatc gttgccattt catcaaaaga agaaaagaca atgataaaaa
    48661 agttacaaat atgttaatga ataaaccaaa tttatcccgt aattatatta cgctgcaaaa
    48721 tccttacaat aaacaggttt ttattgagc
//
LOCUS       DACADA010000016        47783 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.016, whole genome shotgun sequence.
ACCESSION   DACADA010000016 DACADA010000000
VERSION     DACADA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.016"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            71..703
                     /gene="comR"
                     /locus_tag="ISB17_000921"
     CDS             71..703
                     /gene="comR"
                     /locus_tag="ISB17_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAP1978882.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(765..1304)
                     /locus_tag="ISB17_000922"
     CDS             complement(765..1304)
                     /locus_tag="ISB17_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP1978883.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1531..2835)
                     /locus_tag="ISB17_000923"
     CDS             complement(1531..2835)
                     /locus_tag="ISB17_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP1978884.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3234..3776)
                     /locus_tag="ISB17_000924"
     CDS             complement(3234..3776)
                     /locus_tag="ISB17_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1978885.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3799..4824)
                     /gene="nagZ"
                     /locus_tag="ISB17_000925"
     CDS             complement(3799..4824)
                     /gene="nagZ"
                     /locus_tag="ISB17_000925"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP1978886.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4835..5659)
                     /gene="thiK"
                     /locus_tag="ISB17_000926"
     CDS             complement(4835..5659)
                     /gene="thiK"
                     /locus_tag="ISB17_000926"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAP1978887.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5640..6281)
                     /gene="lpoB"
                     /locus_tag="ISB17_000927"
     CDS             complement(5640..6281)
                     /gene="lpoB"
                     /locus_tag="ISB17_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAP1978888.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6295..6672)
                     /locus_tag="ISB17_000928"
     CDS             complement(6295..6672)
                     /locus_tag="ISB17_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAP1978889.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6675..7034)
                     /gene="hinT"
                     /locus_tag="ISB17_000929"
     CDS             complement(6675..7034)
                     /gene="hinT"
                     /locus_tag="ISB17_000929"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAP1978890.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7368..9557
                     /gene="fhuE"
                     /locus_tag="ISB17_000930"
     CDS             7368..9557
                     /gene="fhuE"
                     /locus_tag="ISB17_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAP1978891.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGTYQF"
     gene            complement(9617..11050)
                     /gene="ptsG"
                     /locus_tag="ISB17_000931"
     CDS             complement(9617..11050)
                     /gene="ptsG"
                     /locus_tag="ISB17_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAP1978892.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11345..12142)
                     /locus_tag="ISB17_000932"
     CDS             complement(11345..12142)
                     /locus_tag="ISB17_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1978893.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12153..13157)
                     /gene="holB"
                     /locus_tag="ISB17_000933"
     CDS             complement(12153..13157)
                     /gene="holB"
                     /locus_tag="ISB17_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP1978894.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13154..13795)
                     /locus_tag="ISB17_000934"
     CDS             complement(13154..13795)
                     /locus_tag="ISB17_000934"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP1978895.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13785..14807)
                     /gene="yceG"
                     /locus_tag="ISB17_000935"
     CDS             complement(13785..14807)
                     /gene="yceG"
                     /locus_tag="ISB17_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAP1978896.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14810..15619)
                     /gene="pabC"
                     /locus_tag="ISB17_000936"
     CDS             complement(14810..15619)
                     /gene="pabC"
                     /locus_tag="ISB17_000936"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAP1978897.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15739..16980)
                     /gene="fabF"
                     /locus_tag="ISB17_000937"
     CDS             complement(15739..16980)
                     /gene="fabF"
                     /locus_tag="ISB17_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP1978898.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17068..17304)
                     /gene="acpP"
                     /locus_tag="ISB17_000938"
     CDS             complement(17068..17304)
                     /gene="acpP"
                     /locus_tag="ISB17_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP1978899.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17515..18249)
                     /gene="fabG"
                     /locus_tag="ISB17_000939"
     CDS             complement(17515..18249)
                     /gene="fabG"
                     /locus_tag="ISB17_000939"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAP1978900.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18262..19191)
                     /gene="fabD"
                     /locus_tag="ISB17_000940"
     CDS             complement(18262..19191)
                     /gene="fabD"
                     /locus_tag="ISB17_000940"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP1978901.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19207..20160)
                     /locus_tag="ISB17_000941"
     CDS             complement(19207..20160)
                     /locus_tag="ISB17_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP1978902.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20228..21298)
                     /gene="plsX"
                     /locus_tag="ISB17_000942"
     CDS             complement(20228..21298)
                     /gene="plsX"
                     /locus_tag="ISB17_000942"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP1978903.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21379..21552)
                     /gene="rpmF"
                     /locus_tag="ISB17_000943"
     CDS             complement(21379..21552)
                     /gene="rpmF"
                     /locus_tag="ISB17_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP1978904.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21604..22125)
                     /gene="yceD"
                     /locus_tag="ISB17_000944"
     CDS             complement(21604..22125)
                     /gene="yceD"
                     /locus_tag="ISB17_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAP1978905.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22324..22908
                     /gene="yceF"
                     /locus_tag="ISB17_000945"
     CDS             22324..22908
                     /gene="yceF"
                     /locus_tag="ISB17_000945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAP1978906.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23020..23979)
                     /gene="rluC"
                     /locus_tag="ISB17_000946"
     CDS             complement(23020..23979)
                     /gene="rluC"
                     /locus_tag="ISB17_000946"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAP1978907.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24552..27737
                     /gene="rne"
                     /locus_tag="ISB17_000947"
     CDS             24552..27737
                     /gene="rne"
                     /locus_tag="ISB17_000947"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAP1978908.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27932..28885)
                     /gene="flgL"
                     /locus_tag="ISB17_000948"
     CDS             complement(27932..28885)
                     /gene="flgL"
                     /locus_tag="ISB17_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAP1978909.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28897..30540)
                     /gene="flgK"
                     /locus_tag="ISB17_000949"
     CDS             complement(28897..30540)
                     /gene="flgK"
                     /locus_tag="ISB17_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP1978910.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30606..31547)
                     /gene="flgJ"
                     /locus_tag="ISB17_000950"
     CDS             complement(30606..31547)
                     /gene="flgJ"
                     /locus_tag="ISB17_000950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAP1978911.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31547..32644)
                     /locus_tag="ISB17_000951"
     CDS             complement(31547..32644)
                     /locus_tag="ISB17_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP1978912.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32656..33354)
                     /gene="flgH"
                     /locus_tag="ISB17_000952"
     CDS             complement(32656..33354)
                     /gene="flgH"
                     /locus_tag="ISB17_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP1978913.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33408..34190)
                     /gene="flgG"
                     /locus_tag="ISB17_000953"
     CDS             complement(33408..34190)
                     /gene="flgG"
                     /locus_tag="ISB17_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP1978914.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34362..35117)
                     /locus_tag="ISB17_000954"
     CDS             complement(34362..35117)
                     /locus_tag="ISB17_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAP1978915.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35137..37062)
                     /gene="flgD"
                     /locus_tag="ISB17_000955"
                     /pseudo
     CDS             complement(35137..37062)
                     /gene="flgD"
                     /locus_tag="ISB17_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(37074..37478)
                     /gene="flgC"
                     /locus_tag="ISB17_000956"
     CDS             complement(37074..37478)
                     /gene="flgC"
                     /locus_tag="ISB17_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP1978916.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIQSQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37482..37898)
                     /gene="flgB"
                     /locus_tag="ISB17_000957"
     CDS             complement(37482..37898)
                     /gene="flgB"
                     /locus_tag="ISB17_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP1978917.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38053..38712
                     /gene="flgA"
                     /locus_tag="ISB17_000958"
     CDS             38053..38712
                     /gene="flgA"
                     /locus_tag="ISB17_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP1978918.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            38788..39081
                     /gene="flgM"
                     /locus_tag="ISB17_000959"
     CDS             38788..39081
                     /gene="flgM"
                     /locus_tag="ISB17_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAP1978919.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39086..39502
                     /gene="flgN"
                     /locus_tag="ISB17_000960"
     CDS             39086..39502
                     /gene="flgN"
                     /locus_tag="ISB17_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAP1978920.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(39542..41077)
                     /gene="murJ"
                     /locus_tag="ISB17_000961"
     CDS             complement(39542..41077)
                     /gene="murJ"
                     /locus_tag="ISB17_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAP1978921.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41187..42110)
                     /locus_tag="ISB17_000962"
     CDS             complement(41187..42110)
                     /locus_tag="ISB17_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1978922.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42112..42759)
                     /locus_tag="ISB17_000963"
     CDS             complement(42112..42759)
                     /locus_tag="ISB17_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAP1978923.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42770..43354)
                     /gene="rimJ"
                     /locus_tag="ISB17_000964"
     CDS             complement(42770..43354)
                     /gene="rimJ"
                     /locus_tag="ISB17_000964"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAP1978924.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43590..44798
                     /gene="mdtH"
                     /locus_tag="ISB17_000965"
     CDS             43590..44798
                     /gene="mdtH"
                     /locus_tag="ISB17_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAP1978925.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44862..45509
                     /gene="grxB"
                     /locus_tag="ISB17_000966"
     CDS             44862..45509
                     /gene="grxB"
                     /locus_tag="ISB17_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAP1978926.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45642..46202
                     /locus_tag="ISB17_000967"
     CDS             45642..46202
                     /locus_tag="ISB17_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1978927.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46308..47351
                     /gene="pyrC"
                     /locus_tag="ISB17_000968"
     CDS             46308..47351
                     /gene="pyrC"
                     /locus_tag="ISB17_000968"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP1978928.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVK"
     gene            47428..47673
                     /gene="dinI"
                     /locus_tag="ISB17_000969"
     CDS             47428..47673
                     /gene="dinI"
                     /locus_tag="ISB17_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAP1978929.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11559 a        12906 c        11869 g        11449 t
ORIGIN      
        1 tggactggtg aacggaggtg attattttta taataacctt tcattcacag tcaccaggta
       61 caacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa
      121 agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc agcacggtta
      181 tgaagcgact tctcttgcgg acctcgttga agcgaccgga gctaaagcgc ccacgctata
      241 cgcggaattt accaacaaag aggggttatt ccgcgccgta ctcgaccact atatcgatcg
      301 ttttgccgcc aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
      361 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacactc cggctggctg
      421 cttcatgatc aacaactgca ccaccctctc cccggattca ggagatatcg ccaatacgct
      481 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
      541 gcgcggggaa atcccgaccc actgtgacgt gacgcatctg gcagaattcc ttaattgtat
      601 tattcagggg atgtcgatca gcgcacgcga aggtgcatca ctggaaaaac tgatgcagat
      661 tgcccgaacg actttgcgtt tatggccgga actgctgaaa taggtcgcaa aaaaagcccc
      721 tcagcctgag cgaatgaggg gctaaattgc agagtacagc cttgttatac tttgttattc
      781 aaaatcagct gcccgttgct atctagcggg atctgcgtac ccggatcgcg gtccatgcgg
      841 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
      901 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
      961 gagccctgga tctggttacc tgcatatcca ccccccagcg cccccacaac agtggcgaca
     1021 tctttaccgc gaccacctcc aaactgatgc ccaatcacgc cgccagcaac agcgccgagc
     1081 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtaacgttg
     1141 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
     1201 gcgtattgcg ggccacgttc aaacacgttc agactggcca ctgccgctac gcccagcgca
     1261 gctgcgacac caatcccgat acccgccaac attgacttat tcacgggaac acctccttct
     1321 ttgtagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
     1381 agcgcgggaa tggctggaaa tactcaggga ttcggaggag tctgaaggaa tgcctgatgc
     1441 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
     1501 gtttatgccc catccgccag tacaagtcga ttaatgcaac ttcaaacgtg gacggataac
     1561 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
     1621 ctggtgcatt cggtacagcg agatgtatac aaagcgcgca attcgtcctt caatcatcat
     1681 tgagccgcgc gtcaggttac ccatcaggct accgactgtg gaaaagttcg acagcgatac
     1741 cagcgaaccg tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
     1801 aatgttgttc attgcgcagg ttgccatctg atgtgcggcc tgagcgcgcg gcggaacaaa
     1861 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtatatgt ctggatcgcg
     1921 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttggttt caagaccacc
     1981 gatatctttc aggaagtctg gcgctttgat cccggctgcc cacaccatca gatcagcctc
     2041 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
     2101 cgtcagcacg cgaacgccaa gtttcgttaa ctcactatgg gccgcagcag agatacgtgg
     2161 cggcaatgca ggcaaaatac gttcgcccgc ttctaccagc gttacgttca gggcttcgtt
     2221 ggtcagacct ttataaccgt aactgtgcag ttgcttgacc gcgttgtgca attcagcgga
     2281 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg tttactttgc cattcgcgcc
     2341 caggttggcg gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
     2401 gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct ggtgtattga aatcgttaga
     2461 ggtgctaccc agcgccatta ccagggtgtc ataggcgatt ttacgttccg gaaccagcag
     2521 ttcacctttc tcgtcgcgca gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
     2581 aattacggaa cccagctgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
     2641 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacaa
     2701 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttgcgtc ccagcttatg
     2761 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caattttttt
     2821 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
     2881 taacatagct cttaattaac aacaggttat agcaatgaaa atgttcttcg ctattgagaa
     2941 taacatgaat ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgctgaaa
     3001 agataggcga ctttaccggg tgagatcaaa aaacagagtt tgttgttcat tatttgagct
     3061 gatttgtgag aatgatatag agtgaggtgt gtatggaggg cgttcggtag gcctgataag
     3121 aggcgcatca ggcattgatg ctcgactgtc ggatgcggcg gaacgcctta tccaacccac
     3181 agaatcagga gcgcatcagg taccggcaag gttacctgat gcgacgaggg catttacccg
     3241 agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa tttgtgcgtc
     3301 tgctcttcgt cccagacaat ctcgtaataa tgatgcaact cttcagatgt ccgctggctg
     3361 ttaagcgctt catcattacg cgacaaaatc accaggcaac gatcgcgatt cttctcacgg
     3421 aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc aatcttccct
     3481 tccatgttct cataagggaa caaattgggg ttgaagatca cctggcggat atcacagaga
     3541 aaaccaatcc gctcagccca ataaccgccc agaccaacac cacaaatgag cgggcgctcg
     3601 tcgacgttca gttgcaacat tttgtccact tctttgagca aatgctgcat atcatgtttt
     3661 ggatgccgcg tactgtagct tattagccgt acatccgggt caataaactg caattgtaag
     3721 actttctcgt ggttacccgg actgttagag tcaaaaccgt gcaaatagat aatcatcgct
     3781 tcctcacata agccagggtt aatgacctgc cttctcttcc tgccagcgtt catgtaactg
     3841 attcagacgg gcgctgatcg ctttccagcg agccgagtcc atcagttcct gtcgcgaaaa
     3901 tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca gattatctaa
     3961 cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac ctgcatccag
     4021 tgatgcctgt ccacgctcgg cataactgcc cataatcgct gcgccttcca tcgataaatc
     4081 gtcagagaaa atcacgccgt caaaacccaa ttcctgacgc aaaacggttt tcagccagta
     4141 gggagaaccg ctcgccggac gcggatcaac atcactgtag atcacatgcg caggcataat
     4201 ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt tagcgcgaat
     4261 ctccgcttgc ggacgtgggt cgcacggtgt ttctttgtgt gagtctgccg ttactgcacc
     4321 gtgacctggg aagtgtttcc cggtagtttt cattccggct tcatgcatac catcaataaa
     4381 ccggctggca attgccaggg ctttttgtgg atcggcatga taagaacgct cgccaatcgc
     4441 cgcgctgata tgcccgacgt ccagcactgg cgcaaagctg atatcaatat ccatagcaat
     4501 catttcgctg gccatcaacc aacctgcctc ttgcgccagt tttccgcctt cttccattcc
     4561 caacagcgca gcgaatgatt gtgccgctgg caagcgggta aaaccttcac gaaaacgctg
     4621 cacgcgtcca ccttcctgat ctaccgccac caccagatga tttcgcgatg ctgcgcggat
     4681 ctggcgcacc agttcacgta actgggcagg atcatgatag tttcgcgtaa agagaatcag
     4741 ccctcccacc agcggatgcg ccagtatttc acgctcttcc gcgtccagtt cgtaaccttc
     4801 gacatccaac attactggac ccacactgct ctccttattg ttttattaat agctgccgcc
     4861 aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag cggtactcaa
     4921 accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga cgccataatt
     4981 gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccga tgctggtcag
     5041 tattttccac ccacaccgcc gccagttcca gcgcgatatc accatctccg gcatactccc
     5101 agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg acatccatat
     5161 gcaatggact taagcgtaaa agccgtggtt cccgcgcttt gcgcagacgt tttaacattc
     5221 gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt tccagtaacg
     5281 gcaacagcgt tattcgccag ccaaaacgcg gttgttgatg tagataatac agcaagcctg
     5341 ccagttcgtt ggtatccggc aaatacgttt ttacctcgcc gggcagatag tcaaccacca
     5401 tccagtcacg gagatataaa tgcggcttcg gtgcaatgca tgcgggtagt tgtgataaag
     5461 cccgatactg gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa
     5521 caaaacgctg gccctgatgt tcaatgagaa aactcccgcc actaagccca ctattaaacg
     5581 tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc gtgaggggat
     5641 tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg caccagcatc
     5701 agctgcattt gtagggtcgg agcgttaacg ttgccagagg cgctggagta cagcacgtaa
     5761 tgagcgccaa cattgcgggc aatgcctatg gctttactac gggtgcctaa actgtcctgc
     5821 ggcgacaaac ctaattgttg cttagccatc gacagctgct gggcggaaac cagggtaaat
     5881 ttaccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc attcaacgaa
     5941 ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt cacgccatca
     6001 gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata atggcgaata
     6061 tgcggcgcag gcggtgcagt ttgatcttcg tgctcaattg ggcctggctg ctgcggtatc
     6121 gtcggcaccg atggcactgt ggggacaggc tgttgtggct cggttggttg ttccggcgct
     6181 ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc aagaaacatc
     6241 gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca ccccttacaa
     6301 atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt acagcgtgac
     6361 cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca tctccagccc
     6421 tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc tttcgttata
     6481 aagcgttgag gatgctgaag gttgaatatc agatgtcgaa aggacgggct tttctgcact
     6541 gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat tcaccggaat
     6601 ttccggatgt gaacgacagc ccaccagcac taacaacgcc agagacacca gcccaaagca
     6661 tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg acggccaccc
     6721 aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt gttcatgatc
     6781 agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc tacggtgatc
     6841 atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg aatgaggata
     6901 ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc
     6961 agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt gctgaatata
     7021 gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc atgctacgcc
     7081 gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa ccttaactca
     7141 cgatttctta agcaaaaaac cgttttgctg ccgtatatat cgccattatt cccatttctg
     7201 cctgcgaatg cgctgaaaag acattttctg tttggtttac acaccaaata tgaatgcgta
     7261 tatttctcat ttgcatttac atacaaaatt attcgcacac taaatataac cgctcatcac
     7321 cctgctccgg catgagcctg acgccaacat aaaccaagag atttcaaatg ctttcgacac
     7381 aatttaacag ggataatcaa catcaagcca tcatcaaacc gtcactcctt gccggttgca
     7441 tagcactggc actattacct tccgccgctt ttgctgcacc agccactgaa gaaacggtga
     7501 ttgttgaggg ttcagccaca gccccagatg atggcgaaaa tgattacaac gtaacgtcta
     7561 cctctgcggg tacaaaaatg cagatgactc aacgtgatat tcctcagtcg gtcactattg
     7621 ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg atggaaaaca
     7681 cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat tcccgcggat
     7741 tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg cgctggaatc
     7801 tgggcgacgc actttctgat atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga
     7861 caggcctcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt cgaaaacacg
     7921 cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg aacaaagaac
     7981 ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc gcacgaattg
     8041 tcggcggcta ccagaataac gactcatggc tggaccgcta caacagtgaa aagaccttct
     8101 tctccggcat tgtcgatgct gatttaggcg atcttacgat gctttcagcc ggttacgaat
     8161 atcagcgtat tgatgttaat agcccaacct ggggcggttt accgcgctgg aatactgatg
     8221 gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc tgactgggcg tacaacgata
     8281 aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc tggcaagcga
     8341 cgctgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct
     8401 atgtaaacaa agcggatggt atgctggttg ggccatacag taattatggc cctggttttg
     8461 attatgtcgg aggcaccggt tggaacagcg gcaaacgtaa agttgatgcg ctggatctgt
     8521 tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa tttaatgttt ggtggcagtt
     8581 acagcaaaca aaacaatcgt tacttcagtt catgggccaa cattttcccg gatgaaattg
     8641 gcagtttcta caactttaat ggcaatttcc cacaaaccga caggtcacca cagagtctgg
     8701 cgcaggacga taccacacat atgaaatcgt tatatgctgc cactcgtgtc acccttgccg
     8761 atccgctgca tctgatcctc ggcgcacgtt ataccaagtg gcgggttgat acgctgactt
     8821 acagcatgga gaaaaaccac accacgcctt atgctggact ggtgtttgac atcaatgata
     8881 actggtcgac ctacgccagc tatacctcta tttttcagcc gcaaaatgat cgtgacagtt
     8941 caggcaaata tctggcacca atcaccggta acaactacga gctgggtctg aaatcggact
     9001 ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag gataatgtcg
     9061 ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat aaagcggtgg
     9121 atgggacagt cagtaaaggt gtggaatttg aactcaacgg cgcaattacc gacaactggc
     9181 agctgacgtt tggcgcaacg cgctatattg cagaggataa cgaaggaaac gccgttaatc
     9241 ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct gtcatgccag
     9301 agttgacggt cggcggtggt gttaactggc aaaatcgcgt gtataccgac accgtgacac
     9361 cgtatggcac cttccgcgcc gagcaaggta gctacgcgct ggtggatctc ttcacccgct
     9421 accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc gacaaaacct
     9481 acgataccaa cgtggaaggt tctattgtct acggcgcacc gcgtaattta agcattaccg
     9541 gcacgtatca attctgaata acgcctgtaa aaaaggcagc catctggctg ccttagtctc
     9601 cccaacgtct tacggattag tggttacgga tgtactcatc catctcggtt ttcaggttat
     9661 cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg cccgctgcgc
     9721 ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg acgcgcagac
     9781 gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca gcaaccagag
     9841 ccggtgccat ttcgctggta cctgtcgctt ttgcgtcttc agtcgcgtct tcacgacccg
     9901 gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa acaatggcat
     9961 aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac agaacgatga
    10021 agtcgatcag accgtgcgag aacgacgtac cgtcacgcat ccccagaaga atacagattg
    10081 ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca acgaacatga
    10141 aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc gcggagatca
    10201 taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata gcaattgcgg
    10261 cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc gcagtcgggt
    10321 cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt
    10381 caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc
    10441 aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa gcagcccact
    10501 gagagaaggt ctggattgca gagccaatag gcggccaaat gaaggacagc acaacgccag
    10561 taaagatggc agtcaggcct gaaatgatcg gcacaaagcg tttaccggca aagaagccaa
    10621 gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg
    10681 agataatccc cccgagtacg ccagtatccg ccaggtgttt agaggcgatt tcttcagcag
    10741 gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg ccataggcaa
    10801 caactgcggc cagcgcggat acgccatcgt tattggtaaa gccgagggcg acaccgatcg
    10861 caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata acatgcgata
    10921 caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata cctgcgatag
    10981 gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta gcaaatgcat
    11041 tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg
    11101 gcaagggggg agtgcctcgc cgtgtacagg gcatctaagc gccctttatt tattacacag
    11161 agtaaaataa ttcagtgccg atttgtttga cggctatcac gtttcaactt cagatcattg
    11221 gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg ttattcaccg
    11281 cccgattttc ggcggtgaac tttactcgtt ttagccatta attacgagct ttaaaaaaac
    11341 tcattcaacg gatggattga aggcgggaag catcgatgtg aaacagacgg gcgaagttat
    11401 cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc atgtattctg
    11461 caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtact ggcgcaaggt
    11521 aaggtgagtc agtttccacc agtaaccgat ccagggggac ataacgcgca gcatcgcgca
    11581 gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca
    11641 gtaatttacc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg
    11701 tcactttttc ttcgcgcaga attgccagcg tatcggcgcg ggcgtcacgg gtgtggacga
    11761 taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac tcttgctgac
    11821 gtactttagt ttccggcgtg taataataat ccagcccggt ttcacccagc gctacaacgc
    11881 cctcttctgc cgccagacga cgtaaatctt ctacatcgta gggatcattc tggttgagtg
    11941 gatgcacgcc acaagaaaat acgacgttgt cgcgttcgcc taccagatcc cgcatgtgta
    12001 aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt gcggcggctt
    12061 tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca tcgagatggc
    12121 agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg tagcacaacg
    12181 cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg
    12241 atacctgtga cagacattaa ctgttcacga atgtggcaaa catcccccag tatagcctgc
    12301 aggcgcgagg gagaaagatg gtttgccagt tcgacgacca gccccggcac atcaacattg
    12361 gtcacgtgcg cagcgccata atggcgtttt agcgcatcca tcagcaacgt tgccagccag
    12421 tgtaaacgcg ccggagcttg ttcattatta agggccgcta gcagcgaata ccagtcgccc
    12481 gattgcacgc catatgccag cgcctgacac aatgtttcac gagcctgcca gttatctccc
    12541 tgaaacaacg ccagtgccgc cccaggcgaa ccggcgctta agcgcaatgc ggcaagtagt
    12601 gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg ttccggcggc
    12661 ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg ccagtaaacg ttctggctcg
    12721 cgggtagcaa ggaaaaacca cgtctctgct ggtggctctt caagcgtttt cagcaatgcg
    12781 ttagccgcgg cgtcggttag taaggcagca tcggttaccc agacaacttt cgcaccacct
    12841 aagcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat gcccagcgca
    12901 tttttccctt tttcgggagc cagggtgtag taatcgggat gtgtgccagc ctgcatcaac
    12961 tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca gagtaaataa
    13021 cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg aatgagtagc
    13081 gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac
    13141 catggatacc atctcatgcg tccaactcct tcacccaatt ggtcacggta gtgcggattg
    13201 catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatgctt ttatcttgtg
    13261 ctgccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaatgac tcttgctcaa
    13321 tacgatccag ctcaccacgg gcgcgagcac gttttaagcc gacctctggg gtgacatcaa
    13381 gataaagcgt taagtcaggg cgaaaatccc cgagaacagc atcacgcagt gttgccagca
    13441 tatgttggtc aataccacgt cctccaccct gataggcttg agtggagaga tcgtggcgat
    13501 cgccaatcac ccaggtgccg ttagccagcg caggtttgat gaccgtttct accagttgaa
    13561 cgcgcgcggc ataaaacatc aaaacttcgg ctttatcggt aatgacttca tcgcctaccg
    13621 atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg ccaggttccc
    13681 gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt ctcaaccacc acgttacgcg
    13741 cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg cgcatttttt
    13801 tccttaagca ctttcagata atcctgcaca gacttgttat gactggcaag attggtatta
    13861 aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt tgccggatgc
    13921 gcagcagcct tcagcgaatc cgcccccggc gtcgctatcg cacctggcgg cagaccggta
    13981 atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag tttgccatta
    14041 taacgctctc ccatcccgta aatcacggtc gggtcggtct gcaggcgcat accaatgcgt
    14101 aaacggttga taaatactga ggcaacctga tcgcgttcac tggcaacggc ggtttctttt
    14161 tcgataattg atgccatcgt caccaactgg tttttatctt tataaggcag accgtccgca
    14221 cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg tttgagtaac
    14281 gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca accttcaatc
    14341 cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc gtcgctcagc
    14401 gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag acgcatccct
    14461 tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg
    14521 cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga aagatccggt
    14581 tcgatacgca gcagccattg gaaaacccgt gggcgattga tgatcttatc ggcataaagc
    14641 tgttcaccga gcgccagacg tccggtccct ggcttcaggg taaatatcgt ctcttctttg
    14701 ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc agcggcgata
    14761 cccagtacca ccagcaataa caagattatc aataacactt ttttcatgac taattcgggc
    14821 gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag acatcgccac
    14881 aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca tctgcctgca
    14941 acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt gccagcaaac
    15001 ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc ggcgtataaa
    15061 ctacgttgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc cacccttcgc
    15121 tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcgg atcaatactt
    15181 gctcaagccg attaagatgt ttaatacctg caagatgagg attgcgcccc agccgcaccg
    15241 ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc gtaatgtgca ggataagccg
    15301 taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct cgcccgccac
    15361 taccgcgact gatcacgact ttcagtacac cattttgctg ttctgctgcc agcgttttca
    15421 tctcttgttc aagctgaggc cagaagtcac aggaaatcat caaccgctga caagcatcct
    15481 gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact ctggcggtgg
    15541 taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat tcctgcttat
    15601 gaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg gcagcatgtt
    15661 cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg accttttata
    15721 agggtggaaa atgacaattt agatcttttt aaagatcaaa gaaccattag tgccaccgaa
    15781 gccgaaggaa ttacacagag tgtattccat tccgctaacc tgacgcgctt cgtgcggtac
    15841 gaaatccaaa tcgcaacctt catccgggtt atccaggttg atggttggcg gaacagcctg
    15901 atcgcgcagc gcaaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg
    15961 accggtcata gatttcgtgg agcttaccaa cacgcggctt gcagcttcac cgaagatggt
    16021 tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac
    16081 gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca tcgccagagc
    16141 tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa
    16201 gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag
    16261 taccagcata ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg
    16321 gctcgccgct tgcgggttat cattgcgggt agataatgcg cgtgccgcgc caaaaccacc
    16381 aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc
    16441 atacgcgata atacgcgcag catggccaat gttgtgcacg ccggaagtac aggcggtcgc
    16501 gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg ccaccatgtt
    16561 cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt tcatcagaga
    16621 tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg cggcaccaat
    16681 gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct gaacgccagc
    16741 gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg agataatgtc
    16801 ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa
    16861 atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg tagactctac
    16921 ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac gcttagacac
    16981 gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa aactcaggcg
    17041 gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt aatcaatggc
    17101 agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat caaactcttc
    17161 ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt cttcaacgaa
    17221 agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga taattttctt
    17281 aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg ctttcgcgat
    17341 ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca ggtcttacca
    17401 cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt gcactaatta
    17461 ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac
    17521 catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga
    17581 tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac
    17641 ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat
    17701 aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc gcgccagtga
    17761 tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc
    17821 catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt tcatcatagc
    17881 gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga taatatcgtt
    17941 ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg cattattgac
    18001 caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt cgatagatgc
    18061 cgggtcggtc acattcaaca tcagaccttt gccgttggca cctaaataat cactgatcgc
    18121 ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt
    18181 ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga tttttccttc
    18241 aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg aaggttcgtt
    18301 cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa gcactttgcc
    18361 cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtattcaa cagacttcgt
    18421 ccactgaacc gggttataca actgacgtac cagtgcgtca cgaatggcat caccattggt
    18481 ttcgcatttc acatcaacgt tattcacaac aggaactgtt ggtgcgttaa aggtgatttt
    18541 cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt gagacggtac
    18601 gctcactggt agcggcagag cgcgttttgc gcccgcagct ttacaagcag cacccgcacg
    18661 ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa agtttaccgg
    18721 agaaacgacc tgaccttctg cagcttcttc acacgctttc gcaatagacg catcatccag
    18781 accgatgatt gccgccatag cacccgttcc ttccggtacg gcttcttgca tgaacttgcc
    18841 gcgcatctca accagacgca ccgcatcagc gaaatcaatc acaccagcgc aaaccagcgc
    18901 ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc cctgctgctg
    18961 ccatacgcga tacagcgcaa cagatgcagt caacagcgct ggctgggttt gccaggtttt
    19021 attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc
    19081 agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat cagccagcat
    19141 tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca tgttttaatc
    19201 cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa aggcttcaag
    19261 cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca gagcgcacgg
    19321 gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat tgtccatcga
    19381 catgccgagt tttttcgctg ttgcactgat aatacgcagg ttagcctgat gcggaaccag
    19441 ccagtccagt tgagaacggt caagattatt cgccgtcagc gtctcatcaa cgatgtgcgc
    19501 cagttctgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat gaattgaatt
    19561 ctctggattc acacggtcag cattaggcag cgtcagcaac tcaccatagc taccgtcggc
    19621 atgcagatgg gtggagatga ttcccggctc ttcagaggca gccagcaccg cagcgcccgc
    19681 gccatcgcca aaaataataa tagtcccacg atctgttgga tcgcaggtgc gcgccagtac
    19741 atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt gatcggctac
    19801 gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg ggcaaccttt
    19861 aatgcccagc atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt
    19921 cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct caattgcgcg
    19981 tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc
    20041 gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt tttccaaatc
    20101 ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct tcgtatacat
    20161 gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt tcgctggcag
    20221 cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa ccagctgggt
    20281 atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc gcctgcactg
    20341 cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt tttatcaccg
    20401 tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg tgactgaatc
    20461 gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac gaccgttttt
    20521 tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct tccatcgtct
    20581 ttaatgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg ccagttaaca
    20641 actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta agcaccgctg
    20701 aggcatcccg aatactgtcg agacccttta cttcttcttc accaatattg agcaacgcca
    20761 cgcgaggatt aggaatttcc accacctctt cagccagaac tgagcccata atggcaaatt
    20821 gcaccaacat tgtactgtca caatcgacgt tggcccctaa atcgaggacc accgttttgc
    20881 ccttttgctg atgcggtaat accgtcacca gcgccggacg ctcaatcccc tccaggggct
    20941 tgagtaataa ttttgccagc cccattagcg ccccggtatt accggcactg acacaggctt
    21001 gcgctcgacc ttctttcacc agctccaggg ccacgcgcat tgaactccca cgactggcgc
    21061 ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga ataatctgca
    21121 gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg tcgggattgc
    21181 cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct gcaggcactg
    21241 tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga cgtgtcaagg
    21301 tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct cactaagctt
    21361 catcacgcag acgcgtaatt acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc
    21421 agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca ggctggtgac
    21481 tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg gtttattctg
    21541 ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa cggggagcac
    21601 caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt gcgcttcttc
    21661 aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag aatcatgcac
    21721 cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca gatcgatttc
    21781 accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag cctgttcgtc
    21841 tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg gcttcccgca
    21901 acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg cgagacgttg
    21961 gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga ctacggattc
    22021 ggcgacgcgc tcaacctgat caggagtata gataccctgg taatcaaggc gtttttgagc
    22081 cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc gcgcatatta
    22141 actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt tgccaataat
    22201 tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga acatcaacct
    22261 gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct gatggaaaaa
    22321 aatatgcctg aacttatttt agcctccacc tcgccctggc gtcgcgctct tctggaaaaa
    22381 ctgcaaatct cttttgaatg tgcagcaccc gaggtcgacg aaactccacg cagcgacgaa
    22441 tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct ggcgtcacgt
    22501 tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg cgaaatcacc
    22561 ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag cggcaatatc
    22621 gtcaccttct atactggact ggcgttgttt aattcggcga atgggcatct acaaacagaa
    22681 gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga taattacgtg
    22741 cgtaaagagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt tggcattacg
    22801 ctgtttgagc gtttagaggg gcgtgatcct aacacgctgg ttggtttacc gctaatcgcg
    22861 ctgtgtcaga tgttacgtcg ggaaggaaaa aacccgctga tgggatagtt ttttgcgccg
    22921 gatgcggtta cgtcgcatcc ggcaatcggt gctggcggcc tgatgcggca taaacgcctt
    22981 atcaggccaa ccgcccatta tcatgcttat atgctcgtat tagcgcgcgt tacgcagttt
    23041 ttgcaaacaa cgcttcaaac catcatccat cggcgcttcg atacgcatca cctcaccggt
    23101 ccccggatgg gtaaacttca acgctgcggc gtgcaggaac agacgattta atcccgtgcc
    23161 tgcttcagtg agctgtctgt caaattcacg gtcaccgtag cgatcgtcaa aggcaatcgg
    23221 atgacccgca tattgtgtat gcacacggat ctgatgagta cgccctgtta ccgggctaca
    23281 acgcaccagg gtggcaaatg catagcgctc ttccacttta aagcgtgttt ccgacggttt
    23341 gccttcctga ctcacacgca cgatacgttc gccgctttgc agaatatttt tcagtaacgg
    23401 cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca ggtaatcttt
    23461 ttgcatccct ttttcacgta attgctcatg cagagaacgc aacgccgagc gttttttcgc
    23521 taccagcaac acacctgagg tgtcccggtc aagacgatga accagttcaa ggaaccgcgc
    23581 ttccgggcgc aacgcccgca aaccttcgat aacgccgaag cttaaaccac taccgccatg
    23641 taccgccgta ccggaaggtt tattcagcac caggatatgg tcatcttcat ataagatgac
    23701 gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt cccgttcagc
    23761 aacgcgaacc ggtgggatac gcacctcatc acccgcttcg agtttatatt caggcttaat
    23821 acgttttttg ttcacccgca cttcgccttt acgcaaaata cggtaaatca tactttttgg
    23881 tacacctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt cgtcagcggt
    23941 gatagcaaca atttttacgg atggagtctc tgttttcatg gtgggcgatt ctaattagcc
    24001 aacaggattc gcgccactca tttttctatg cttatattta gttttgcacc ttattacttc
    24061 actacatgat cactttattg atggttatta aaccaatcac cagcaagaag tgaaaaaact
    24121 gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct tgcagtggaa
    24181 taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg aataacccat
    24241 tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt tcaggcagtt
    24301 agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag agtaataccc
    24361 agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa ccgacactct
    24421 gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca cgaggccatc
    24481 ggttcacacc cggaaaggcg ttactttgcc cgcagcttag tcgtcaatgt aagaataatg
    24541 agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag ttgcgcgttg
    24601 cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg cacgagcaga
    24661 aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg gaagctgctt
    24721 ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt gcccgcgaat
    24781 atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg ttgcgtgaag
    24841 gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc gcggcattaa
    24901 ccacctttat cagtctggcg ggtagctatc tggttctgat gccgaacaac ccgcgcgcgg
    24961 gtggtatttc tcgtcgtatc gaaggcgacg accgtaccga attaaaagaa gcactggcaa
    25021 gccttgaact gccggaaggc atggggctta tcgtgcgcac cgctggcgtc ggcaaatctg
    25081 ctgaggcgct gcaatgggat ttaagcttcc gtctgaaaca ctgggaagcc atcaaaaaag
    25141 ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta atcgttcgcg
    25201 cattccgcga ttacttacgc caggacatcg gcgaaatcct tatcgataac ccgaaagtgc
    25261 tcgaactggc acgtcagcat atcgctgcat taggccgccc ggatttcagc agcaaaatca
    25321 aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg cagatcgagt
    25381 ccgccttcca gcgtgaagtt cgtctgccat ccggtggttc cattgttatc gacagcaccg
    25441 aagcgttaac ggccatcgac atcaactccg cacgcgcgac ccgcggcggc gatatcgaag
    25501 aaaccgcgtt taacaccaac ctcgaagctg ccgatgagat tgctcgtcag ttgcgcctgc
    25561 gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgccagta cgccaccagc
    25621 gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgggcgcgt attcaaatca
    25681 gccatatttc tcgctttggc ctgctggaaa tgtcccgtca gcgcctgagc ccatcactgg
    25741 gtgaatccag ccatcacgtc tgcccgcgct gctccggtac cggtaccgtt cgtgacaacg
    25801 aatcgctgtc gctctctatt ctgcgtctga tcgaagaaga agcgctgaaa gagaacaccc
    25861 aggaagttca cgccattgtt cctgtgccaa ttgcttctta cctgctgaat gaaaaacgtt
    25921 ctgcggtaaa tgcgattgaa actcgtcagg acggcgtacg ctgtgtgatt gtgccaaacg
    25981 atcagatgga aaccccgcac taccatgtgc tgcgcgtgcg caaaggggaa gaaaccccaa
    26041 ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg tctgaagaag
    26101 agttcgctga acgtaagcgt ccggaacaac ctgcgctggc aacctttgcc atgccggatg
    26161 tgccgccagc accaacgcca gctgaacctg ccgcgcctgt cgtagcccca gcacctaaat
    26221 ctgcaccggc aacaccagcc gctcctgccc aacctgggct gttgagccgc ttcttcggcg
    26281 cactgaaagc gctgttcagc ggtggtgaag aaaccaaacc gtccgagcaa ccaacaccga
    26341 aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag aacaaccgcc
    26401 gtgaccgtaa tgagcgccgc gaccctcgta gtgaacgtac tgaaggcagc gataatcgcg
    26461 aagaaaaccg tcgtaatcgt cgccaggcac agcagcagac tgccgagacg cgtgagagcc
    26521 gtcagcaggc agaggtaacg gaaaaagcgc gtaccaccga cgagcagcaa acgccgcgtc
    26581 gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa gtgaaggcgc
    26641 tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta cgtccggttc
    26701 agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa agcgtagccg
    26761 aagaagcggt agtcgcaccg gtggttgaag aaactgtcgc agccgaacca attgttcagg
    26821 aagcggcagc cccacgcaca gaattggtga aagttccgct gccagtcgta gcgcaaactg
    26881 caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc atgccgcgtc
    26941 gttctcgccg ctcgcctcgt cacctgcgcg taagtggtca gcgtcgtcgt cgctatcgtg
    27001 acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcgcg tctccggaac
    27061 tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat gtacaggttg
    27121 aagagcagcg cgaacaggaa gaagtacaag tgcagccgat ggtgactgag gtccctgtcg
    27181 ccgccgctgc cgaacctgtt gttagcgcgc cagttgttga agaagtggct gaagtcgtag
    27241 aagcccccgt tcaggttgcc gaaccgcaac cggaagtggt tgaaacgacg catcctgaag
    27301 tgattgctgc cgcggtaact gaacagccgc aggtgattac cgagtctgat gttgccgtag
    27361 cccaggaagt tgcagaacac gcagaaccgg tggttgaacc gcaggaagag acggcagata
    27421 ttgaagaagt tgccgaaact gctgaggttg tggttgctga acctgaagtt gttgctcaac
    27481 ctgccgcgcc agtcgtcgct gaagtcacag cagaagttga aacggtagct gcggttgaac
    27541 ctgaggtcac cgttgagcat aaccacgcta ccgcgccgat gacgcgcgct ccggcaccgg
    27601 aatatgttcc ggaagcaccg cgtcacagtg actggcagcg ccctactttt gccttcgaag
    27661 gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc atctgccgct cctgcgcgtc
    27721 cgcaacctgt tgagtaatag ttagctcaag taatcaagcc ctggtaactg ccagggcttt
    27781 tttatttcat ctttgagtca acatattaaa attcatcaaa tggcatcctt gctaaccaac
    27841 aatacaaaat aggcaaaaaa atgcccgctc cggcgatccc agagcgggca gacaaaacat
    27901 acccagtttc atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc
    27961 cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag
    28021 atgaaatagt tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta
    28081 aagcgcgatc gctacctaat gaatccagcg actccagttc gttcagttgc gtgcctaatt
    28141 ccgcgcgaac agtcagcaca ttgttcagcg agtttttcag tccgcggttg gttttatcca
    28201 gtgccgcagc ggcggtttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg
    28261 cgatggcgct atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg
    28321 ctaccgcgtt gctagtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg
    28381 aacgcgaagc atcgacctgt tgtttaatac tttctgctcc gccttcgtat ttgccatccg
    28441 cctcgctaaa cggtgcggat tctgttttat aaccggcaaa aatgtagcgc ccgttaccgt
    28501 cagtggtgtt cgccagattc agcaactggt cacgaagccc ctgaatatcc gtagccagcg
    28561 aggcccggtc atcgtcactc aaggtgccat tgctggcgta gacaattttt tcctgagcat
    28621 tctgtatagc ggttgtgacc tggctaagta cactctcttc cagtgacact ttttgagtgg
    28681 cgaaagtacg cgccagcgtg tactggctgt tttgcgcctg tgcctgggag agaacaacag
    28741 cttgtgatgc agcaatggga tcatctgaag ggttaacgac tcgcttaccc gtcgacatct
    28801 gttcgccgta cttcatccat tctgcctgag aattggtgat gccgcgcatg ttttgctggt
    28861 acatcatctg ggtactgaaa cgcattttat ctccccttag cgaatgttaa tcagcgcatc
    28921 aaaaatcgcg tttgccgtct gcagaacctg cgcattcgcg aggtaatact gctgaaaacg
    28981 ttgcagattt ccgtactcct catcgagatt gacaccggaa atcgactgct gctgattgga
    29041 aagctgcgtc accacattac cttgagtggt gctactggtt ttcaacgtcg cggttttatt
    29101 accgatatca ctcactaacg aggcataagc gtcgttaaag gatttcgcac cgcccaccgt
    29161 tttactgttg ctttgcagat cgagcagggc ctgaccgttg cggttgtcgc tatcacccgc
    29221 atcttctgcg ctcgccatgg cgattttcgc ttcgtcggtg attaatacat ccatgttgac
    29281 gatggcgtca cttaccggtt tcagcgtgaa gctgtcgtta acggcaggcg ttcctgtaaa
    29341 cgtcaactcc agaccatcaa atgccacttt accgttggca tccggcgtca ccgtaaaagt
    29401 ggtattgctg gcaaggcggg tgacctgcca ctgattatta tcgaacgaga ttttgtaatc
    29461 tgtcgccagt accgcggagg tatcagttac cgttgcaccg atcgcaacgt cacctttgtt
    29521 tttcgtgttt tgcagaaccg cgggcttacc gatagcaaag aaatcttcac cggcatcgcc
    29581 gttagcatca aatccggctt tgtgttgggt gttgaaagcc tcggcaaatg ccagcgccag
    29641 ttgtccaagc gtattacgcg tctggtccag atcctgagaa cggaatgtca gaatgccgcc
    29701 cagcgacccg gtattcagta atttctccgg gatctcaata ttgcctgccg tcccatcaac
    29761 ataagcgaca gtcgtacgag aagggtcggc gctggaagga actgccgcaa gttgccgcgc
    29821 cgtacttccc tgaaccagtg agtagccatt ggccatcgtg atgttataag tgccaccatc
    29881 ctgaacactg acttctacgc caacaatctg gtttaattcg ctcaccagtt gatcgcgttg
    29941 atccagcaga ttgttaggtg acgcccctgc ccccacgcct gtcaggcggg agatctgatc
    30001 gttcaggctg gcgatttgtt tagcgtaatt gttgatctga tcaacactgg cacctatcgc
    30061 gatattgacc tgtttgtcct ggtcgcgcag atattgatcg gtggttttaa actgattcac
    30121 caacccttct gatttcccaa tcagcgcctg gcgcgctgcc ggatcttccg cgttactcac
    30181 cagcgtttgc aggctggtga agaaatcctg catctgtgtt gccagcgaag aggtactggt
    30241 ggagagcata ttgtcgattt tcgacatctg ctcatagcgg gcagtcaggc cgctactttg
    30301 cgtctgcgcc gcacgtaact ggttggtaat aaacgcatca tactcacgct gcacaccaga
    30361 aacgtagacg ccattgccaa cccagccgcc agcgcccaac gtgctattgg cctgcgccat
    30421 aatagtggtt tggcgggtat atccggcaac gttatagctg gagatattat tacttgccgt
    30481 atttaacgcc gcctgggccg cgttcagtcc gctcatggcg ttattgatca agctggacat
    30541 ggaggttcct tataagcctt caattacaga gtattatcgg cagcgacccg ccggacttga
    30601 gttattcaga acagattatc aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc
    30661 gatttcatct gctgaatcat gttggtgagt ttgcgggcat agtgaggatc ggtggcatag
    30721 cccgcgtcct gtagggcctg cgccccctgc tccgcactcg cggcggtcgt cacggcggcg
    30781 tagcgcgggt tacgcgttaa cagcccaacg taatccgaca atgcttccag atacgagctg
    30841 tagacccgaa acttcgcttt tactttcttc gcttcgccat tttcatattc agtcgtggtg
    30901 atttcagtga ctggcccttt ccagttgcca gaggctttga caccaaacag gttatagctc
    30961 ggctcgccgt tttcacggcg gatttgccgt tgtccccagc cagattccag cgccgcctga
    31021 gcgaggatca aatgatgtgg cacaccactt tgctggcttg ccagttgggc gggcagcgag
    31081 agttgcgcga ggaatgcttt actgtcaccc ggcagcgaat catcgtagtt acgtggtacg
    31141 gccttttgca ccagctgcga aagcgtctga ttttgataac gcaccacggt ttcgagcggg
    31201 aattttatcg gtgctgctgg cgtggactcc tctggcaatg gttgttctgg cgtcatctgt
    31261 ttaaccatca tctctgccag ccccagacct ttgcccgccg tcatctgttg ggcaatctgc
    31321 tggtcataca tactggtata caggcgagtg tgctcgctgc tgaacaggcc atcttttggt
    31381 aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca tcccttccac ctggcgggcc
    31441 accggacgga tatttgccgc tggatcttcg cccgctttcg cctttagttc gttgagtgat
    31501 tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc tgatcatcag atgatttcca
    31561 gttttgcccg cagacatccc gcactttgca ttgattgcag tatggacatc agatccatcg
    31621 gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt gaggctggcg ctggaacgta
    31681 cgctttgcag cgaaccgccg ctctggcgta aatcgatctg cgtttgtgga gtaaccacag
    31741 tctgcccacc accaaacggt gtatctggct ggctgacatt ggcctgacga ttaactgtta
    31801 ctgagagatt cccctgcgct accgcgcagc tgtcgagggt cacttcgcga ttcatcacca
    31861 ccgaaccagt gcgcgagtta atcactactt tagcgtcctg cggggtgaca ttaacctgca
    31921 tattctggat atcggcaagg aaacggacct gggaactgtt gccacttggt acgcgcacct
    31981 gaatagtccg cgcatctaac gcggtggcgc tgccatatcc acgcacgcgg ttgatggtgt
    32041 cagcgatttg ctgcgccata ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc
    32101 cgacgccaaa ctggctgggc aattcacgtt caataaccgc accattggtg atccgtccac
    32161 cgttcagttg attcacctga acactgctac cgccagcgga ggcccctgcg ccgccaacca
    32221 gaatattgcc ctgcgccagc gcatacacct gactgtcaac gcccttaagc ggtgtcatca
    32281 ataacgtacc gccacgcaga cttttggcat ttcccatgga agaaaccacc acgtcaatgg
    32341 tttgcccctg acgtccaaac ggtggaagtg acgctgtcac cataaccgca gcgacgtttt
    32401 ttagctgcat attagtgccc gtcggaacgg taattcccag ctgtgagagc atgttattaa
    32461 gcgtttgtgt ggtaaacggc gtctgagttg tctggtcacc tgtaccatca agccccacca
    32521 ccaggccata gccaatcagt gagttttgcc ttaccccctg aacactggtg agatcgcgaa
    32581 tacgctcagc ctgagccgcc gtggtaacca gtaaaagaat taatgcagag agaaatttaa
    32641 tcaccacagc ctcacttaca ttggcgacag gttaaggaag aaacgctgca accagcccat
    32701 attttgcgct tcgttaatgt agccattgcc tacgtattca atgcgcgcat ctgccacctg
    32761 agtagacggt acggtattgc tgccgctgat agtgcgtgga ttaaccaccc cagagaagcg
    32821 aataaattcg gtaccctgat taatggcgat ctgtttttca cccaccacat gcaggttgcc
    32881 gttgaccagt acctggtcaa ccgtcaccgt caacgtaccg ctaaaggtat tgctggcatt
    32941 ggccccgcct tttccgttga acgtgttacc accggaggct tcgacatcgg cacgagcgtt
    33001 accaaacaac ccctgcaaat agcgcggcac agtatcaaag ccaaaattag ttttaccgtc
    33061 acggctggca ttcgcagagg agcttttgct ggcgctgacg ttctcctgca acacgatagt
    33121 cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac agcggttgat agccatagtt
    33181 aatcggctga gcagactgga aaatagaccc gttggcgacg ggcgtcggac cgggaaccgg
    33241 ttgtgcactg gtcgccccct gtaccagcgg cgtggagggt atccaggcgc agccggttag
    33301 tgaaagcgcc aacaagctgg aaatggcata agtatgcgca gcgttttttt gcatggctat
    33361 catcttcaaa aatcagtaaa ccggtgaacc tgcccaccgg ttaagcctta gagttgcgtc
    33421 agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt
    33481 tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac
    33541 ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tgcaccggag
    33601 gattgcgttt cggtgtagag gttttcgcca atgctctcca gtccggtgtc attcataaag
    33661 gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg
    33721 accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc
    33781 tgcacctgaa aaccaccggc cgtcaccagc tgcccgttct gatccacctg gaaagagccg
    33841 tcacgggtat aggctgatga gccatctggc aacatcacct ggaaaaagcc ctgcccttta
    33901 atcgcgacgt ctttactgtt attggtttgc gacaggttgc cctggctgtg taaacgttcc
    33961 gtggcgacag ggcgtacccc cgtaccgatt tgtaatccgg atggtaaggt ggtttgttcg
    34021 gaagactgtg cccccggctg gcgaatggtt tgataaagta gatcttcaaa caccgcgcgc
    34081 tgacgcttaa aaccgttggt actaacgttt gccaggttgt tggcaatgac gtccatattg
    34141 gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat aaaatgtcct
    34201 gtagtggatt agtgacaaag tgcatatgtc ttcttgccgg atgcggcgta aacgccttat
    34261 ccggcctaca agagctcgca aaatcgataa attgcgattg cccccataga cccgataaac
    34321 ttgcgcatag ggcaacttat acttgtcatg tatcctttca attaactcat cgacagcagt
    34381 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
    34441 cgccgtgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
    34501 agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggccc acgcgtagcc
    34561 tggctttctg cgcttaaacg aaaaatgcca tcgtcaccgc gctggacttc gctgcccgtg
    34621 gctttcacca gcttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattc
    34681 agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg cacagcaatt
    34741 ggccccgcct cgcctaccac cggatgtccc tgaattgtca gttgcccggt gggatcaacc
    34801 tgaatgctgc cattacgcgt atacccttca ctgccgtcag cggtctgcac gaccagccaa
    34861 ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    34921 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    34981 accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    35041 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    35101 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtatt
    35161 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    35221 gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt
    35281 cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    35341 agcggaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    35401 tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    35461 atcattgatt tgataactca ccagatcgcc cggtttgtag ccgttctggg tggttgccac
    35521 aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    35581 gggatctgcg ccgttaattg cgccggtggt aatatccttt gttggatctg aggtcagaac
    35641 gccattggca ttaaacacca gcgtcgttgc agggttagct gtgcctgttg gatcactgct
    35701 atccagggtg taaacatccc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    35761 atgagcatta ccctgactat cgaaaacagt caccgaacct tttttgttat agctatccgc
    35821 attgctggca tcaaatgcgt taacagaggg aagcgaatca ctggaattca ggttgatctg
    35881 catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcggaatcg aaatattggt
    35941 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    36001 taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    36061 gtagaatacc gaaccattgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    36121 gtccagacca cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccagc
    36181 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    36241 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    36301 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    36361 gaaggcttaa attatctgcc gtacttcgtc gagggtagtg gtgccgtaag tgccaagatc
    36421 cagcgtatta ccgttgttgc cgcggatcac cccctgcacc agagcaaact gcagcggttg
    36481 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    36541 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtggactc cggcagtcag
    36601 ttcaccgata tcaatggtac ggacgaccgc gccgttttta tcggtgatgg tggccgtcac
    36661 tttgtctgct gcctgttgca actcaacacc aaacggcgtg gtcgtggtca cagccccttc
    36721 ttcactgccg gttcccgcaa gaactgtggt accggggatc atcacgccgt gaccgataag
    36781 gttactggcc tgtaacgact gactgttatc aatctgtccg gaaatagatc cgagcgtggt
    36841 attgagtttt tcaatcccac tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    36901 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa
    36961 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    37021 gccggtattc gtcggatcgg tggtggttac cgcaatagac atagctttct cctttattga
    37081 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc
    37141 tgataactgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgtttggc
    37201 attttcacgt agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc
    37261 ggggcctgac tttgtataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    37321 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcggg accggtcacg
    37381 ctatcagcat tcgccagatt actggccgcc acgttcagac gctgggactg ggcagttaac
    37441 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    37501 acgttcatca tgcctttaat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    37561 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cagtattacc gtcaagcgaa
    37621 ggctggtccg gaatacggta ttgcagttct gcggaaggag gcgtcagcgc ctgcgccgga
    37681 atgtgttgcg ttgaggtcat cgtcagagca accacactgg ttgcatcccg tccacgttgc
    37741 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    37801 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    37861 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    37921 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    37981 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    38041 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actcttcagt
    38101 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    38161 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct gctaccgccg
    38221 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    38281 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtacaggc cacaggaaat
    38341 tatgtggttg ccgcgatgcc cattgtgcgt ggaggaaagc tggaagctgg caatgtcaaa
    38401 ctgaaacgcg gacggctgga taccttgcca ccgcgtacgg tgctggatat caatcaactt
    38461 gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccacttt
    38521 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaacg ttatcgccag cggtgatggg
    38581 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    38641 gtgcgcatga tatcgggaca agtggtcagc ggcgttgttg atgcagatgg gaatattctt
    38701 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    38761 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcca
    38821 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacaaacac ccgagcggca
    38881 aaaacaacag cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    38941 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaagct ggcgattcgt
    39001 aacggtgaac taaaaatgga cactggcaaa attgccgatg cgctgatcaa cgaagcgcag
    39061 caagacttgc agagtaattg atcgtatgac gcgtcttgca gagatcctcg accagatgtc
    39121 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    39181 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    39241 gctggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat
    39301 tagtcaacgc tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    39361 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    39421 accgcatcag gaaccgacgc tgtatggggc gaacggtcag acctcaaccc cctatcgcgg
    39481 cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatgtatc
    39541 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtactgcc agtgcagcga
    39601 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaaacgc cagggcatgg
    39661 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    39721 gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt
    39781 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    39841 aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta
    39901 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    39961 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    40021 cccgctgggt catcagcgca tcaaaggcgg taaatttacc gtactggaac agcgaaacgg
    40081 ttaacggacc ggaaagaatg cccaacgcaa ccgcacttgg cagcgccaac aggaaacaaa
    40141 gacgcagccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    40201 tcgacagcga cggtagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    40261 ccattaagcg gtcggcgtaa tacatccacg acaccgaacc ggaagcaaga aacgaggcaa
    40321 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatt gccggtccca
    40381 tctgtttcac cacacgcatt gctccggcat cgtggaagtt aatgcgtggc aaaaccagca
    40441 tgccgatctt cttcagatga ggtagctgat acaccagctg caggatgccg ccgaccgtaa
    40501 cagcccaggc cagcgccagc accggtggat taaagtacgg cgcggcaaac agcgcaaaac
    40561 caatcatgct gatattgagc aatgtcgggg caaacgccgg aattgagaag cggttccagg
    40621 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtta
    40681 tctttagtag ctggctggtc agggcaaatt tgtcggcagt gtcagcgaag cctggcgcgg
    40741 tcaccatgat cacccacggt gcagcgagca tgccagcgac cgtcacaacc gccagcgcaa
    40801 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttgc
    40861 ttttatattc tgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    40921 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    40981 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    41041 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    41101 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    41161 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    41221 acgatacgtt gcgccagcac ggcctgttcg ccggctgttt gcggaactgt ctgattttgc
    41281 acgcattcaa tgaagtgccg cgcacagtcg acaaatccac gttgctcaag cgtactctgc
    41341 caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata
    41401 tccgtaatgt cgatcagcgc accatcagtc acggcctgca cggtttcacg ctgacttccg
    41461 gcccggcgat gcatacaggt ggtgatctgc aaaggaccag ccgaaaaatg gtgctcggca
    41521 aacagcattt cgccggactc attggtcagc aacgtaccgc tttcgagagt tgcattgccg
    41581 ccagacaacc acagcgcggt atcaaccaca tgcaaataat catccagcaa tgtgaaataa
    41641 agatcgtgtg gcccgacgct attggtacga tgtttatcca ttcttagcga ggacgcggtg
    41701 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    41761 aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    41821 gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgcca
    41881 aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat
    41941 aacgaatcag cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta
    42001 ggcgaccagg caccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    42061 ttttgcgcga tgccgcctaa ccccactacg ccgatacgta attttttcac tttaatctcc
    42121 cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    42181 ttcgacgcgg gcctgtaaat caccgtcaac cgcgttcgac atatccgtta ccgccggcgg
    42241 attctcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    42301 ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc
    42361 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg
    42421 cgtctgggca ccacgcaata acagcgtggt gattaacgcc acttctgcgg cgctcagttt
    42481 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    42541 accgctcacc gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    42601 agattcactc agattcataa ccggttcacg gttcgttttc tgattacagg ccgtgactac
    42661 accatttacg gagagcgggt attgctccgg cgtggtcacc tgtttttcca gcaagcaacc
    42721 aatcacgcgg gcttccaggg cggtcagttg atatttcatg agattctcct tagcggccgg
    42781 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    42841 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    42901 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    42961 tataacgaat ggctgcgctc agagcttcaa acatgagtcc tttgccctgc catttttgcc
    43021 caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg
    43081 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    43141 gtttatgaaa ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc
    43201 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    43261 ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    43321 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    43381 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccagaa
    43441 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    43501 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    43561 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg aggaacctgg
    43621 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    43681 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    43741 ccctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    43801 accgctttgg tgccaaaccg atgattgtca ccggtatgct gatgcgtgcc gccggattcg
    43861 ccacaatggg tatcgcccac gaaccgtggc tactgtggtt ttcatgcctg ctttcgggac
    43921 tcggcggcac gttgtttgat cctccgcgtt cggcgctggt ggtgaaacta atccgtccac
    43981 aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    44041 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    44101 cagttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccagca tggaaactct
    44161 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgcgac aagcgttttg
    44221 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    44281 tgccaattat ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca
    44341 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    44401 ttcgtctgga acaccggtta atggctgggc tgttgataat gtcattaagc atgatgccag
    44461 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    44521 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcagac gcaagagctc
    44581 gcggcagcta tatgggattt agccgtctgg gtctggcgat tggcggcgct attggttata
    44641 tcggtggcgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    44701 tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag tttagccaga
    44761 aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc ccctgactcc
    44821 tccatactga aagagaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    44881 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    44941 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    45001 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gacatcgttc
    45061 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    45121 aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca
    45181 aatcggcatt tgatgagttt tctactcctg ccgcgcgcaa atatttcgtc gacaagaaag
    45241 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    45301 tcagcgatga tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    45361 aactttcgga agatgatatt cagctatttc cgctcctgcg taatctgacg ctagtagccg
    45421 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    45481 tcaatttgtt atcatcaatg gcgatttaat cctgacccgt cgcggcagcc tgtattcgcg
    45541 ggttgccgcc gttttcagtt ttttctgctg gcgcgttatc gctggtgaca catgctgcgt
    45601 tgtgtcagga tattgacgca gatttcatgt cgaggactac catgaacaag tttttatttg
    45661 ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc caatacacca
    45721 tcaccgaaca agaaattaac cagtcgcttg cgaaacataa taatttctca aaagatatcg
    45781 gtttacccgg cgtggctgac gcccatatcg ttctgacaaa cctgaccagc caaattggtc
    45841 gcgaagagcc gaataaggtt accctaaccg gagacgccaa tctggacatg aactccctgt
    45901 tcggtagcca gaaagcgacc atgaaactga agctgaaagc gctgcccgtg tttgataaag
    45961 aaaaaggcgc gatcttcctg aaagagatgg aagtggtcga tgcgacggta caaccggaaa
    46021 aaatgcaaac ggtgatgcaa acgttgcttc cctatttaaa ccaggcatta cgcaattact
    46081 ttaaccagca acctgcttac gtcctgcgcg aagatggcag ccagggcgaa gcaatggcga
    46141 agaaactggc gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg tttactgatt
    46201 aatcacgagg gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat
    46261 cctttgtgtc cggcaaaaac atcccttcag ccggagcata gagattaatg actgcaccat
    46321 cccaggtttt aaagatccgc cgcccagacg actggcacct tcacctccgc gatggcgaca
    46381 tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg acgggctatc gtaatgccca
    46441 atctggctcc gcccgtgacc accgtagagg ctgccgtggc gtatcgccag cgtattcttg
    46501 acgccgtacc tgccgggcac gatttcaccc cgctgatgac ctgttattta acagattcgc
    46561 tggatcctaa tgagctggag cgcggattta acgaaggcgt gttcaccgct gcaaaacttt
    46621 atccggcaaa tgcaaccact aactccagcc acggcgttac gtcagttgac gcaatcatgc
    46681 cggtacttga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtaacac
    46741 atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaaccgc
    46801 tgcgccagcg cctgaccgcg ctgaaagtcg tttttgagca tatcaccacc aaagacgctg
    46861 ccgactatgt ccgtgacgga aatgaacggc tggctgccac catcactccg cagcatctga
    46921 tgtttaaccg caaccatatg ctggttggtg gtgtgcgtcc gcacctgtat tgtctaccca
    46981 tcctcaaacg caatatccac caacaggcat tgcgtgaact ggtcgccagc ggttttaatc
    47041 gtgtattcct cggaacggat tctgcgccac atgcacgtca tcgcaaagag agcagctgcg
    47101 gctgcgcggg ctgtttcaac gccccaaccg cgctgggcag ttacgctacc gtctttgaag
    47161 agatgaatgc tttgcagcac tttgaagcat tctgttctgt aaacggcccg cagttctatg
    47221 gcttgccggt caacgacaca ttcatcgaac tggtacgtga agagcaacag gttgctgaaa
    47281 gcatcgcact gactgatgac acgctggtgc cattcctcgc cggagaaacg gtacgctggt
    47341 ccgttaaata ataaaaatcg tagcttcctg ttgtcattag gttattttac ctgtataaat
    47401 aaccagtata ttcaacaggg ggctattatg cgaattgaag tcaccatagc gaaaacttct
    47461 ccattgccag ctggggctat tgacgccctg gctggcgaac tttcccgccg tattcagtat
    47521 gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg ccgcagcaaa taatttatcg
    47581 gttattggcg cgacaaaaga agataaacag cgcattagcg aaattctcca ggagacgtgg
    47641 gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccggg
    47701 tcgccccggc ttttttattt tacttgcgaa ttgtgagtac aaaatttcta cacttactca
    47761 aaataattgc acaatatgct aaa
//
LOCUS       DACADA010000017        47653 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.017, whole genome shotgun sequence.
ACCESSION   DACADA010000017 DACADA010000000
VERSION     DACADA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.017"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            386..1603
                     /locus_tag="ISB17_000970"
     CDS             386..1603
                     /locus_tag="ISB17_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP1978930.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1687..2286
                     /gene="yfbR"
                     /locus_tag="ISB17_000971"
     CDS             1687..2286
                     /gene="yfbR"
                     /locus_tag="ISB17_000971"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAP1978931.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2345..4177)
                     /locus_tag="ISB17_000972"
     CDS             complement(2345..4177)
                     /locus_tag="ISB17_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAP1978932.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4264..4914)
                     /gene="hxpA"
                     /locus_tag="ISB17_000973"
     CDS             complement(4264..4914)
                     /gene="hxpA"
                     /locus_tag="ISB17_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAP1978933.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4925..5419)
                     /locus_tag="ISB17_000974"
     CDS             complement(4925..5419)
                     /locus_tag="ISB17_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP1978934.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5502..5957)
                     /locus_tag="ISB17_000975"
     CDS             complement(5502..5957)
                     /locus_tag="ISB17_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAP1978935.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6295..7497
                     /gene="ackA"
                     /locus_tag="ISB17_000976"
     CDS             6295..7497
                     /gene="ackA"
                     /locus_tag="ISB17_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP1978936.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7572..9716
                     /gene="pta"
                     /locus_tag="ISB17_000977"
     CDS             7572..9716
                     /gene="pta"
                     /locus_tag="ISB17_000977"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP1978937.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9906..11426
                     /gene="yfcC"
                     /locus_tag="ISB17_000978"
     CDS             9906..11426
                     /gene="yfcC"
                     /locus_tag="ISB17_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP1978938.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11459..12001)
                     /gene="yfcD"
                     /locus_tag="ISB17_000979"
     CDS             complement(11459..12001)
                     /gene="yfcD"
                     /locus_tag="ISB17_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAP1978939.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12059..12610)
                     /gene="yfcE"
                     /locus_tag="ISB17_000980"
     CDS             complement(12059..12610)
                     /gene="yfcE"
                     /locus_tag="ISB17_000980"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAP1978940.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12666..13310)
                     /gene="yfcF"
                     /locus_tag="ISB17_000981"
     CDS             complement(12666..13310)
                     /gene="yfcF"
                     /locus_tag="ISB17_000981"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAP1978941.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13446..14093
                     /gene="yfcG"
                     /locus_tag="ISB17_000982"
     CDS             13446..14093
                     /gene="yfcG"
                     /locus_tag="ISB17_000982"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAP1978942.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            14150..14512
                     /gene="folX"
                     /locus_tag="ISB17_000983"
     CDS             14150..14512
                     /gene="folX"
                     /locus_tag="ISB17_000983"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAP1978943.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            14533..15426
                     /locus_tag="ISB17_000984"
     CDS             14533..15426
                     /locus_tag="ISB17_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAP1978944.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15585..16358)
                     /gene="hisP"
                     /locus_tag="ISB17_000985"
     CDS             complement(15585..16358)
                     /gene="hisP"
                     /locus_tag="ISB17_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAP1978945.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16366..17082)
                     /locus_tag="ISB17_000986"
     CDS             complement(16366..17082)
                     /locus_tag="ISB17_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1978946.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17079..17765)
                     /locus_tag="ISB17_000987"
     CDS             complement(17079..17765)
                     /locus_tag="ISB17_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP1978947.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17855..18637)
                     /gene="hisJ"
                     /locus_tag="ISB17_000988"
     CDS             complement(17855..18637)
                     /gene="hisJ"
                     /locus_tag="ISB17_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAP1978948.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18858..19640)
                     /gene="argT"
                     /locus_tag="ISB17_000989"
     CDS             complement(18858..19640)
                     /gene="argT"
                     /locus_tag="ISB17_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAP1978949.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19906..20475)
                     /locus_tag="ISB17_000990"
     CDS             complement(19906..20475)
                     /locus_tag="ISB17_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP1978950.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(20570..22087)
                     /gene="purF"
                     /locus_tag="ISB17_000991"
     CDS             complement(20570..22087)
                     /gene="purF"
                     /locus_tag="ISB17_000991"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP1978951.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22124..22612)
                     /gene="cvpA"
                     /locus_tag="ISB17_000992"
     CDS             complement(22124..22612)
                     /gene="cvpA"
                     /locus_tag="ISB17_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAP1978952.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23053..23715)
                     /gene="dedD"
                     /locus_tag="ISB17_000993"
     CDS             complement(23053..23715)
                     /gene="dedD"
                     /locus_tag="ISB17_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAP1978953.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(23705..24973)
                     /gene="folC"
                     /locus_tag="ISB17_000994"
     CDS             complement(23705..24973)
                     /gene="folC"
                     /locus_tag="ISB17_000994"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP1978954.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25043..25957)
                     /gene="accD"
                     /locus_tag="ISB17_000995"
     CDS             complement(25043..25957)
                     /gene="accD"
                     /locus_tag="ISB17_000995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAP1978955.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26113..26772)
                     /locus_tag="ISB17_000996"
     CDS             complement(26113..26772)
                     /locus_tag="ISB17_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1978956.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(26855..27667)
                     /gene="truA"
                     /locus_tag="ISB17_000997"
     CDS             complement(26855..27667)
                     /gene="truA"
                     /locus_tag="ISB17_000997"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP1978957.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(27667..28680)
                     /locus_tag="ISB17_000998"
     CDS             complement(27667..28680)
                     /locus_tag="ISB17_000998"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP1978958.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(28746..29882)
                     /gene="pdxB"
                     /locus_tag="ISB17_000999"
     CDS             complement(28746..29882)
                     /gene="pdxB"
                     /locus_tag="ISB17_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAP1978959.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            29981..30976
                     /locus_tag="ISB17_001000"
     CDS             29981..30976
                     /locus_tag="ISB17_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAP1978960.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(30973..32151)
                     /locus_tag="ISB17_001001"
     CDS             complement(30973..32151)
                     /locus_tag="ISB17_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HAP1978961.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32416..33636)
                     /gene="fabB"
                     /locus_tag="ISB17_001002"
     CDS             complement(32416..33636)
                     /gene="fabB"
                     /locus_tag="ISB17_001002"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAP1978962.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            33795..35801
                     /gene="mnmC"
                     /locus_tag="ISB17_001003"
     CDS             33795..35801
                     /gene="mnmC"
                     /locus_tag="ISB17_001003"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAP1978963.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(35922..36200)
                     /locus_tag="ISB17_001004"
     CDS             complement(35922..36200)
                     /locus_tag="ISB17_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAP1978964.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(36234..36782)
                     /locus_tag="ISB17_001005"
     CDS             complement(36234..36782)
                     /locus_tag="ISB17_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAP1978965.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(36782..37591)
                     /locus_tag="ISB17_001006"
     CDS             complement(36782..37591)
                     /locus_tag="ISB17_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAP1978966.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(37591..38415)
                     /gene="mepA"
                     /locus_tag="ISB17_001007"
     CDS             complement(37591..38415)
                     /gene="mepA"
                     /locus_tag="ISB17_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAP1978967.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38419..39504)
                     /gene="aroC"
                     /locus_tag="ISB17_001008"
     CDS             complement(38419..39504)
                     /gene="aroC"
                     /locus_tag="ISB17_001008"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP1978968.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(39539..40471)
                     /gene="prmB"
                     /locus_tag="ISB17_001009"
     CDS             complement(39539..40471)
                     /gene="prmB"
                     /locus_tag="ISB17_001009"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP1978969.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            40637..41188
                     /gene="smrB"
                     /locus_tag="ISB17_001010"
                     /pseudo
     CDS             40637..41188
                     /gene="smrB"
                     /locus_tag="ISB17_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899504.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
     gene            complement(41248..42072)
                     /locus_tag="ISB17_001011"
     CDS             complement(41248..42072)
                     /locus_tag="ISB17_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAP1978970.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(42074..42601)
                     /locus_tag="ISB17_001012"
     CDS             complement(42074..42601)
                     /locus_tag="ISB17_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1978971.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDAASTAQGVGIGMEKTDGTAVLINNTSGATFVLSSGNNDLHFNAWLQAKSGRDVT
                     MGDFTASLTATFEYI"
     gene            complement(42598..43077)
                     /locus_tag="ISB17_001013"
     CDS             complement(42598..43077)
                     /locus_tag="ISB17_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1978972.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINLSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(43074..43565)
                     /locus_tag="ISB17_001014"
     CDS             complement(43074..43565)
                     /locus_tag="ISB17_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1978973.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGTTTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(43582..44334)
                     /locus_tag="ISB17_001015"
     CDS             complement(43582..44334)
                     /locus_tag="ISB17_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1978974.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(44354..47002)
                     /locus_tag="ISB17_001016"
     CDS             complement(44354..47002)
                     /locus_tag="ISB17_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1978975.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQSLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGISGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDEDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDASQSDMARTGNDKE
                     MYTITYNQNFAAAGVSVYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNTDKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQINVG
                     TSEQHGSVDGYLSHDGTLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGINAGEHMMVFWEGSAQCEIVLPKPLPADLFRGLLLPCEQKGTTAPDSLAP
                     EIKPVIQDQTRQVTPSEAPTSISATQ"
     gene            complement(47083..47649)
                     /locus_tag="ISB17_001017"
     CDS             complement(47083..47649)
                     /locus_tag="ISB17_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1978976.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        11543 a        12982 c        11944 g        11184 t
ORIGIN      
        1 attccgctta acaatagttt cacaccttgc ccccattgtg acacctcgga aatcatcacg
       61 ctgttaaact ctccgtaatg cggaaatatc atcatcgcga ttcagtagtg aattcttaaa
      121 cgaggcattt tcacacaatt atcttacaga taaaaaaacc acacttacaa ttaagaatca
      181 gaacaattca ctacataaca ttacttatta agcatatgca cctcattatt ttgccattat
      241 taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc tgcagacctt
      301 caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca gggagtggcg
      361 ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca aattagagaa
      421 tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg aagaagaagg
      481 taacaaggtt ctgaaactga acatcggcaa ccctgccccg ttcggttttg acgcgccaga
      541 tgaaattctc gttgacgtga tacgcaacct gcctaccgct caagggtatt gcgattccaa
      601 aggtctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg gcatgcgtga
      661 tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat
      721 gcaagcattg ctgaacagcg gggacgaaat gttggttcct gcacctgatt acccactatg
      781 gaccgcggcg gtttcactct ccagcggtaa agcggtgcat tatctttgcg atgaatcctc
      841 tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta cgcgtgggat
      901 cgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc ttttaatgga
      961 gattgtggag attgcacgtc agcataatct cattatcttt gccgatgaaa tttatgacaa
     1021 aattctctac gacgacgctg agcatcactc cattgcgccg cttgcacctg acctgctgac
     1081 cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat
     1141 ggtgttgaac gggccgaaaa aacacgctaa aggttacatc gaaggtctgg aaatgctggc
     1201 ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg cactgggtgg
     1261 ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc agcgtaaccg
     1321 cgcgtgggaa ttgatcaacg atattccggg cgtttcctgc gtgaaacctc gtggtgcgct
     1381 gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt cacgacgatc agaaaatggt
     1441 gctggatttc ctgttgcagg aaaaagtcct gttggtgcaa gggacggcat tcaactggcc
     1501 gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt
     1561 gagtaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat ttcactgccg
     1621 gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat catacgtaag
     1681 gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact cattaaccgc
     1741 tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt gcaggtagcg
     1801 atggtcgccc atgcgctggc agctatcaaa aatcgtaaat ttggcggtaa tgtcaacgcc
     1861 gaacgtatcg ctttactggc gatgtaccac gatgcttcag aagtgctcac cggagatctc
     1921 cctactccgg tgaaatactt caattcgcaa atcgctcagg aatacaaggc tattgaaaaa
     1981 atcgctcagc aaaaactggt cgatatggtt ccggaagagc tacaggatat ctttgcgccg
     2041 ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca
     2101 ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga attcttgctg
     2161 gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat ggactacttc
     2221 atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca ggattcaccg
     2281 ctgtaagcag ctggagtctg cgtcgcatca ggcaataagc gccggatgcg acatcaggct
     2341 cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc acgataatgg
     2401 tgaacggtac ccccaactta acaaagtcac tgaagctgta attccccgga cctaaaacca
     2461 gtgtgttaac aggtgaagat atcggtgtca taaaggcggc ggatgctgcc atcgccacga
     2521 ccatcgcaaa tggataaggc gacaccccca tcgttttggc agcagccagc gcaatcggag
     2581 ccatcaacac cgccgtcgca gtattagaaa taaatagccc aataaccgcc gacaagacaa
     2641 acaaacaacc cagcatcata tgtggcccat aaccgccgcc aatgtccatc agccctttca
     2701 ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc atcccaacga
     2761 tcaaaataat gctcggccag tgaatggatt tataggcact ttcggcatcg atacagcgga
     2821 atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt tcatcggtca
     2881 gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg
     2941 gcgatgcttc actcacctct tccggtaagt tcagcgccac gaagtcgcgg ccctgtttgg
     3001 ccagcatacc gatcagtttc cagttaccaa caaccagaat gatatcgccc agcagcagag
     3061 gctcatccgc cagcgaacct tccagcgcca caccattgcg ctttagcccc accacattca
     3121 atccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaataagt tctgactcag
     3181 gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa tactcgccgc
     3241 gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg acatcagccg
     3301 cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca ttaacgttca
     3361 cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagca ccataacgtt
     3421 cacgtaattt gagatcgtcc agtcgttgac caatcattgg cgatccgggg cgaatagcca
     3481 gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag gttcggcgcg
     3541 tccagccttc gcgttgtggg gtctgggtat cccctttcag catgaaacgc atcactaaca
     3601 tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta aagaaactga
     3661 agccgtgata gccttcacgc agtaattcac tgttgactac caggttcggc ggcgtcgcca
     3721 ccagcgtcat catgccgctt atcagcccgg caaaacttaa cggcatcatc agacgcgacg
     3781 gcgacgtttg catacgcatg gcaacgctta acaccacggg gataaagata gcgacaacgc
     3841 cggttgaact cataaacgcg ccaagtcccg cgacggtcag catcaacaaa accaacattt
     3901 tgatttcact gttgcccgcc actttgacca gccatgtccc cattacagtg gcaacaccgg
     3961 tacggaccaa accatcgcca ataatgaaca aggcggcaat caggacaacg ttaggatcgg
     4021 aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac gcgacaataa
     4081 caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg atggcaacgg
     4141 ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt taattgaggg
     4201 ggataacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc atgatcaggt
     4261 gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt gctccagact
     4321 gtggaggact aaatcgactt cattcaggcg cggggtatct gccggggcat taaccgcaat
     4381 aacatggcaa cccgccgcca gacctgaaag cacgccagcg ggagcatctt ccaccacaac
     4441 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
     4501 ttttccgcgc ttcacccgct cagcggttac aaacacctct ggcgcgggaa gccccgctat
     4561 tttatgacgc gctcgcgcta ccggcatgga gccagaagtc acaatagccc acggaatacc
     4621 tgctttattc aaatgattga gtaaggcgat tgcccccgga agcgcggtaa taccttcggt
     4681 ttccgtggcc tcgatgtgtt ccagacgcgt aaactcggcg gcaatgtctg cctcggattt
     4741 gcccgccata aaatggcgca gagaggtgat cgcctgttta ccgtgaatga aagccagcac
     4801 ctcttccggc gataacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg
     4861 cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc
     4921 ctcctcaggc gttaataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc
     4981 aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa
     5041 aaccgtgagt tccagcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga
     5101 cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt
     5161 catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct
     5221 ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca
     5281 gttcacgcat ctgtaatcca taaccacgct caatgattgt ttgcaggcga cggtaacgtt
     5341 cagcatttgc cggatcgagc atagtcatca tcttgtactg gttagacaaa ataagacgtt
     5401 gcgcgttggt catttccatt attgactcct gtatcactct actacggtga aaaaaaagaa
     5461 ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa
     5521 gttttatcca gttgtttgaa ggcacgctta agcgtgtcag ctaatgcctg gtaatcaggc
     5581 ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa
     5641 aaccagttga ggattgcagg aggtaatggc gttacagaac gcttgcccag ccaccacaat
     5701 ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa ctgccggccc aagctgaccg
     5761 cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca
     5821 taacgcgtca tcttgataac gcgattttcg acaaagactg gggcaaggcg tttttccagc
     5881 ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc taaaaaaatt aacagaacga
     5941 ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca
     6001 aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt
     6061 atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata
     6121 cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc
     6181 atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta
     6241 cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg
     6301 agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat
     6361 gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct gcccgaagca
     6421 cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct
     6481 cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct
     6541 gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc
     6601 gtagtgattg atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg
     6661 cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa
     6721 gacaaaaacg ttgctgtatt tgacaccgca ttccaccaga ctatgccgga agagtcttat
     6781 ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac
     6841 ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa
     6901 gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac
     6961 ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc
     7021 cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc
     7081 gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg
     7141 accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca
     7201 atggacgttt actgccaccg cctggccaaa tacatcggtg cctacactgc gctgatggat
     7261 ggtcgtctgg acgctgttgt attcaccggt ggtatcggtg aaaatgccgc gatggttcgt
     7321 gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg
     7381 gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt
     7441 atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt
     7501 cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa
     7561 gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga
     7621 ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt
     7681 tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg
     7741 tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag
     7801 gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcatcgcg aactaccacg
     7861 ctaacaccaa agacgctgaa gtcgttctgg tggaaggtct ggtcccgaca cgtaagcacc
     7921 agtttgccca gtctctgaac tacgaaatcg ccaaaacgct gaacgcagaa atcgtcttcg
     7981 ttatgtctca gggcactgat actccggaac agttgaaaga gcgtatcgaa ctgactcgca
     8041 acagcttcgg cggtgcaaaa aacaccaata ttaccggcgt tatcgttaac aaactgaacg
     8101 ctccggttga tgagcagggt cgtacccgtc cggatctgtc cgagattttt gacgactcca
     8161 ccaaagcaaa agtgaacaac gttgatccgg cgaagctgca agaatccagc ccgctgccgg
     8221 ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc
     8281 gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg
     8341 tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc
     8401 tggtgacttc cgcagaccgc cctgacgtgc tggttgccgc ttgcctggct gccatgaacg
     8461 gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta
     8521 aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct
     8581 ggcagacttc tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgagc
     8641 gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgattctc
     8701 tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt tatcagctga
     8761 ctgaacttgc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac gaaccgcgta
     8821 ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta
     8881 atccggcaga gatcaaccgt gttgcagcct ctcagggtgt agaactgggt gcaggcattg
     8941 aaatcgttga tccagaagtg gttcgcgaaa actatgttgg tcgtctggtc gaactgcgta
     9001 agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggttctcg
     9061 gtacgctgat gctggaacaa gatgaagttg atggtctggt ttccggtgct gttcacacca
     9121 ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg
     9181 tatcttccgt gttcttcatg ctgttgccgg aacaggttta cgtttacggt gactgtgcga
     9241 tcaacccgga tccgaccgca gaacagctgg cagaaatcgc gattcagtcc gctgattccg
     9301 ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg
     9361 gtgctggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc
     9421 ctgatctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga
     9481 aatccaaagc accgaactct ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc
     9541 tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgctgacctg atctctatcg
     9601 gaccgatgct gcagggcatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg
     9661 atgatatcgt ctacaccatc gcgctgactg caattcagtc tgcacagcag cagtaatctc
     9721 gtcatcatcc gcagctttgc gctgcggata tctgaaccgg aaatagtcac tatttccggt
     9781 tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact gcgctgcatc
     9841 aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa gggaatattt
     9901 gcgctatgtc cgcaatcact gaatctaaac caacaagaag atgggcaatg cccgatacgt
     9961 tggtgattat cttttttgtt gccattttaa ccagccttgc cacctgggta gttccggtgg
    10021 gcatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag
    10081 tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc
    10141 gcgtacagct gttcacgacg ggcgatgaac gcccgggtct gatgaacttc ccgtttgaag
    10201 ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt atgctggtga
    10261 ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc
    10321 ttattcgcca tacccgcgga aatgaaattc tctttattcc tgcgctgttt attctgtttt
    10381 cacttggcgg cgcaatattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg
    10441 caccgctaat ggtccggctg ggctatgaca gtattaccac cgtccttgtg acctatattg
    10501 ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt gttgctcagg
    10561 gtattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg
    10621 ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc
    10681 ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggct gatgttgaac
    10741 aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct
    10801 gggttatttg gggcgtgatc gttaatgcct ggtttattcc tgaaattgcc agccagttct
    10861 tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg
    10921 ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc cctgccctgc
    10981 tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca
    11041 gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg gataacgcag
    11101 tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg acgtccggtt
    11161 ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta
    11221 accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac atcatttacc
    11281 caacctcagc ttcgttaatg gcgacgctcg gtgtttgccg ggtggacttc cgtaactggc
    11341 tgaaggtggg tgcgaccctg cttggactgc tgtttattat gtccagcgtc gtggtgatcg
    11401 gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcgattgcgg ctcttttttc
    11461 attctgccgt ttcagtctcg acggcttcat ttttggcatt gcgcttcatc cacaacgcca
    11521 gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca
    11581 gccagcaaac ttcactgact tcatcttcct gtagagcgaa gggaccgtga gagacgcagc
    11641 tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg
    11701 caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca
    11761 gctgctcatc ggcctggact acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt
    11821 cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatat
    11881 aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga
    11941 cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca
    12001 ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt
    12061 acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta
    12121 cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc
    12181 cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt
    12241 acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat
    12301 gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga
    12361 aatgcagcag catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat
    12421 gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat
    12481 tacgcgggcc atgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg
    12541 caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca
    12601 tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt
    12661 tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc
    12721 cactggaatg tcgcataatc caccaggcgt tccggcacct catcgccatg tagtaccagg
    12781 cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc
    12841 tgacccagtg ctaacaaatg ctctgccatc gcgaacagtt tctctgcact ggcttttccc
    12901 tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg gcgttcttcg
    12961 cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt
    13021 tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac
    13081 tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg
    13141 cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg
    13201 gtcttgatat gaaacgacag gcctttctcc tgcaacgcca cccaggcgga taacacataa
    13261 ggggagaaaa aatgggcatc tgaccaaagc gtaatagcgg gtttactcat tatgtcctcg
    13321 gcaataaagg gggcttgcta tcaaactata gagccttttt gtcactgtca cgtgacgaat
    13381 actcacgcaa gtcgccgcgc tttctatact tgtggtgttc agatgattag acaaccggag
    13441 ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc
    13501 tcgaagaagc agggctggat tatcgcttga taaaggtaga cctggggaaa ggaggtcagt
    13561 ttcgcccgga atttttgcgc atttcgccta acaacaaaat tccggcaatt gttgatcatt
    13621 ctccagccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc
    13681 tggctgagaa aacgggactc tttttgaatc atgaaacgcg tgaacgcgcc gtcacattac
    13741 agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta
    13801 atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc
    13861 gtctgtacca tgtactgaac aagcggctgg aaaattcgcc ctggctggga ggcgagaact
    13921 acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg
    13981 acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca
    14041 ccgggcaggc actgctaaaa gcacaacacg gtgatgagcg ttcggatagt taacagaaac
    14101 aggttctcgt gtattatttc attctaagta aaacaacgga gaacctgcaa tggcacaacc
    14161 tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga
    14221 agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa
    14281 agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat
    14341 tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc aggatgtgct
    14401 cgatatcgca cgcgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca
    14461 cgcgctgcgc tacaccgatt cggtatccat gaccttaagc tggcagcgtt aaccaccata
    14521 tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg
    14581 attccacgtt tgctggacct gggccatcaa atcacggtag tgacgcgtaa cccgcagaaa
    14641 gccagttccg ttctcggccc ccgagtgaca ctatggcaag ggcttgccga acaaagcaac
    14701 ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg
    14761 actcacgagc gaaaagagcg tctctgccaa agccgctgga ataccacgca aaaactggtc
    14821 gatttgatta atgccagcga cacgccaccg tcggtactca tttccggctc ggcaacgggc
    14881 tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt
    14941 acccataaac tctgcgcccg ctgggaagag attgcctgtc gggcgcaaag tgacaaaacg
    15001 cgagtgtgcc tgctgcgtac cggcgtagtg ctggcaccag acggtggtat cctcggtaaa
    15061 atgctgccgc catttcgttt tggcctgggc gggccgattg gctccggtcg gcagtatctg
    15121 gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga taacgagctg
    15181 cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg
    15241 ctcggtcatg cgctgcatcg cccgaccatt ttgcgcgtcc ccgcgaccgc cattcgactg
    15301 ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc aaaaaagctg
    15361 gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt
    15421 cgctgatgtg gtttactgca aacatccgcc agcggaaacg ctgatgaata aatatagata
    15481 attcagaact atttttagcg gactgcaact tttacatttc agcggaagcg gactcgcaaa
    15541 ttccccatga aatactccgg cagcgttaac caccggagag gaaattattt cagcgaacct
    15601 ttaaggaact gttgcagacg agggctttgc gggttaccga ataactgctc cggcgcccct
    15661 tcttcttcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg acgagcaaag
    15721 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
    15781 cgcaacactt cgcctaccag ttcaggatcg agcgctgaag taggttcatc aaacagtaaa
    15841 acttccggtt ccatcgctag cgcccgcgcg atagaaacac gctgttgctg accaccggac
    15901 aggtgcaccg ggtatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttc
    15961 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    16021 acgttttcca gcaccgtcat atggctccag agattgaagt gctgaaatac catcgtcagg
    16081 cgtgtgcgca ataagcgcag ctgattttta tcggcgactt tgagttgacc atctttatcg
    16141 cgcaccagat tgatcgtctg gccattgacc acgatcgatc catcgctcgg tttttcgagg
    16201 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatg
    16261 acatctccgg cattcgcttg caatgagacc cctttcagca cttcgtgttc gccgtagcgt
    16321 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    16381 aggtttcaca tgctgcaacc agcgtttttc agctctacga aagaggctga tcagaacata
    16441 agagatgatt aaatagagca ccgcggcaat gccgaaagcg gtaaaaggtt gatatgtggc
    16501 ggcgttaata tcgcgggcaa ttttcagcag atccggcacc gtggcagtaa atgccagcgc
    16561 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    16621 agaaggcaaa ataatgcagc gatacatttt aaaggtcgag aagccatagg ctctggcggc
    16681 ttcaatttcc ccatgcggca ctgaacggat tgccccggca aaaatctcgg tagtgtaagc
    16741 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgaa aaaaggcgtt
    16801 aaggaactcg gttcccttaa caatctccag cgtgtacatg ccggaataga acaccagcaa
    16861 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    16921 gtatttatta cttgagacgc gaccaatcgc cagaaacagc gccagaactc cgcctatcac
    16981 taccgacaaa atcagcagcc acagggtgat cgccacgcca gtaaagcgat aaccgtcagt
    17041 ccacagcagt ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
    17101 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaaca
    17161 agataaatca cgccgcagac gatagcgaaa tagaacggtt cccaggtact tttgcctgcc
    17221 agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    17281 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    17341 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
    17401 cctttcggca ctgccataaa cgcgccacga aacgtttcgg taaaataagc accgtagata
    17461 aaaccgagag tgataatacc tgcgaccatc ggatcaatat caatctgccc gacgcccatc
    17521 gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaatact
    17581 aaatccggca cgcctcgaat cagcgtggtg tacccttcga aaataaggcc cgaaagccga
    17641 ttttgtgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
    17701 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacttga aaacccatac
    17761 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    17821 ccgaagggag cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga
    17881 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
    17941 tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
    18001 acagtttttc atctttaaca gacgggccac cgaatttgta atctttaccg acgggttgtt
    18061 tgaggaaacc ttcgctggct gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    18121 tcaggtcaga ataaatgttg tcctgcccct gataagaaac gatttcaatg ccttttggtg
    18181 cccagtgttc gttaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    18241 ctttcagcga ttcgactgtc ggctgaatgt cggaattttt cgccaccacc agacgagaat
    18301 cggctgcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
    18361 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagtg
    18421 ggttttcgac aaacgtacat tgcgtattga tgcgtttgca caactccttg gccagatcga
    18481 tatcgaagcc aaccagttca ccttgtgaat tttttgattc aaatggcgca taggtcggat
    18541 cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc tgcagttgcg ctggagaagg
    18601 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    18661 ttatcgggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    18721 aaaatatccc gccccgctag acgtattatt tttcattaaa aatgcttaag tatgcatttc
    18781 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    18841 agcttccctg cgacgtatca gtcaccgtag acattaaagt caaaatactt tttcgccatc
    18901 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattaaacgc agccgtcagt
    18961 tccgcatcat ctttacgtag ccctacaccg gtgccgtcac cgaagtattt tttgtctttc
    19021 actgatgggc ctgcaaaggc gaaatcttta ccggcgggtt gcttaaggaa accttcgctg
    19081 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagact
    19141 aaatcctggt tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg
    19201 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    19261 gttggctgaa tcggtgaacc tttggcggca atcaaacgag aatctgcggc gtacagcttg
    19321 tcggagaaag caatctcctg ctgacgttta tcggtaatgg agagcgacga aataatggcg
    19381 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcgctggc aacccaggta
    19441 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaag
    19501 tcacctttgg catctttcga tgagaacggt gcgtaggtgg tatcggttcc gatacgtacc
    19561 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
    19621 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
    19681 ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacagcagt
    19741 aacattcaac gttaaatatg ttaataagaa gttgcattat tgtcctaaag ttgaagatag
    19801 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
    19861 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
    19921 gcgggcaaag agatcttcag gaagggttac cgcaaactgg tcaagaacac gattaaccgt
    19981 ctgatttatc acatcatcaa gggattgtgg gcgatgataa aacgccggaa cgggaggcat
    20041 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcagtgg
    20101 cgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
    20161 ggtcagtaag ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg
    20221 taaaatcacc atccccaacg tctggaaaga accggaagaa atgctggcag caatatcgcg
    20281 cgcatcgtgt gtcacatcag ctaacgcctg cacttcgcgc agggaaaaat ccgtttcgag
    20341 ggataaggtc tggcgcgccg cctggctcat taccagatgc gtttcgatat ctgtgacatc
    20401 gcgcagaacc tgtaataaac gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
    20461 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcaggatttc
    20521 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctga gcgaaggcat catccttcgt
    20581 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcgtcattac
    20641 gtaaagtatc gaggaaatcg aggtagccct gatcaacgtc tttggtgacg tagacgccgt
    20701 tgaacaccga gcattcaaac tgctggatat ccgggttttc agcgcgaacg gcttcgatca
    20761 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcta
    20821 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcgataccg taaacgttcg
    20881 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
    20941 ccatctcgat aatctgctca gaagtggtgc cacggacgat agagtcgtcg accagcagga
    21001 cgtttttatc gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac
    21061 gcagctgctg acccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct
    21121 ggcggtacgg tttgcccaga atacgcgcaa tttccagcgc gatatcgcag gaagtttccg
    21181 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca
    21241 gcttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg
    21301 ggcgagcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacact
    21361 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgccaggg gcgacatcac
    21421 gcaggaactc aaagcccagc gtatcgagcg caacgctttc ggaagcgacc atatattctg
    21481 tgcgattatc gtcaatatca cgtttgccca gtacaagcgg acgaatcccg ttaggatcgc
    21541 ggaaagcaac cataccgtgg ccgataatca tcaccacaca ggcatacgcg ccgcggatta
    21601 agcggtttgt agcagcgatg gcggcgaaaa tgttgtcggc ttccagcggg tagtggcgga
    21661 agttgtccag ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
    21721 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
    21781 cgttgtgggc aagcgtaatg ccatacggag agttaacgta aaacggctgc gcttcagagg
    21841 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
    21901 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgctttc cgcaaacgga
    21961 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
    22021 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
    22081 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
    22141 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
    22201 tcagctgtga tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
    22261 agagaatggc agcaacaatc aacacaccgc gcaaggcacc gaaacagacg cccaacaccc
    22321 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag
    22381 caccaacgat cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt
    22441 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
    22501 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
    22561 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
    22621 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
    22681 gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
    22741 tcgacttttt atgcctttgc ggcatcgggc aatgcgtgtc ggatgcggcg tgaacgcctt
    22801 atccgaccta cggctctacc cctgcacagg cctgataaga cgcactggcg tcacatcagg
    22861 caacggctgt cggatgcagc gtgaacgcct tatccgacct acggttctac ccctgcacag
    22921 gcctgataag acgcgctggc gtcgcatcag gcaacggctg tcggatgcgg cgtgaacgcc
    22981 ttatccgacc tacggttcta cccctgcaca ggcctgataa gatgcgccag cgtcgcatca
    23041 ggcaaaaccg tattaattcg gtgtatagcc cattaccaca ccacttaagc cagaaagctg
    23101 cttgagctca cccagcgatc ctttcagctt ctctttggaa gcatctggcc caaccagaat
    23161 acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc
    23221 gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgcgc ccagttgcac
    23281 aacataggct ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt
    23341 cggcgcaggt ggtgcttcaa ccttcggttt aggcgactcc accggtttcg gcttcggtgg
    23401 gacgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc
    23461 gagcgacggt gctgcggcat cgcctgcccg cacctcttcc gctgcgcctt ccggcggctg
    23521 cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcgcgat cgcccgcttt
    23581 cggcaccagc gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag
    23641 ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg
    23701 aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt
    23761 ggaacgaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc
    23821 atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg
    23881 ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca
    23941 accaggccag agttccggca atatctttat catgtagcat accgataacc gccagcacgc
    24001 gcccgttttt cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat
    24061 gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa
    24121 ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ctccagcccg ctggcacgca
    24181 gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca
    24241 gcgtgccgtg agcatcgcta aacgtccagt catgatcggt gacggaatag ttccactcaa
    24301 cgccccgacg ttgtagcagt gcaccttttt cctgccccac atcagcaatg gtagaaggca
    24361 tttccggctc accgacaatt gccggttttt cgctgcgaaa gatacctgct ttctcgcgac
    24421 caatactttc gcgatctggc cccagccagt cggtatgatc cagcgcaata ctggttacta
    24481 ccgcgacatc ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca
    24541 gaatcactac gtcaagttgt gcctgcttga acagccacaa tgccgacagc gtaccgtact
    24601 cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaagcggtgt
    24661 gggccgattc cggcaattcc tgcccctgca cacgtacgcg ctcggtataa cgcaccagat
    24721 gcggcgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac
    24781 ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca
    24841 gaacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac
    24901 tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc
    24961 gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca
    25021 gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc ggtggcacca
    25081 ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa
    25141 tgctcgccag tttcagacgc atttccggac gacggacgat catgtcgatc gcgcctttct
    25201 cgatcaggaa ttcactgcgc tggaatcctg gcggcagttt ttcgcgaaca gtctgttcga
    25261 taacacgcgg accggcaaag ccaattaacg ctttcggttc agcgatgttg agatcgccca
    25321 gcatggcgaa acttgcagaa acaccgccca tggtcgggtc ggtcagcacg gagatgtacg
    25381 gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg
    25441 acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agtgggcagt
    25501 tatcttccag cgcctgctca acggcacgca cgaaacgcgc acctaccaca gaccccatgg
    25561 aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc
    25621 ctttcatcac caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt
    25681 ctttatactt cttggagtca cgaaacttca gcacatcttt cggctcaagc tcgctaccca
    25741 gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca
    25801 tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa
    25861 cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg
    25921 gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct
    25981 ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca
    26041 tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg
    26101 cagagttact ttttattttg cggcgcgtgc cgctgcgcgt ttgtggcgaa taaattcgaa
    26161 aacacctggc aatattgaaa caaaaataat gccaacaatc atcagtttaa ggttgctctg
    26221 aatgaagggg agcgtgccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag
    26281 tgcgccaatc acgttataag cggcgaaatg acggtacgac atgtggccca ttcccgcaac
    26341 aaacggggcg aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc
    26401 atgtttctca taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt
    26461 gggattactg aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc
    26521 cacaatcgcg gcgatcagca tcagcaccac catcatatgg acgttgagat cgttggtctc
    26581 cagtgatgcc aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcgttac
    26641 caccagaccg gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccgta
    26701 ctccgcgacc agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata
    26761 aatcaggtcc atatttgcct atgccttgta ctcgtcatct atcaggctgt aactacgttg
    26821 gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca
    26881 ttggcggttt tggaagatca tatcggtcag ggtaatccac cgcgaccaga tatagccctt
    26941 ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc
    27001 agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca
    27061 tatgatgtac aaaggcattc gctttgatat ctaccaccac ataaggaccg tgacgcgtga
    27121 cattaatatg cataacgttg cgccacgggg tccgggactg gcactgcacc gcacggaacg
    27181 aggtgaaatc attctcgccc agcaaacatt gcgcagcccg atgcatccgt tcagcgtcca
    27241 gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc cgatgattgt
    27301 agatgatgta gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta
    27361 cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaacgtcc
    27421 acgcagcgtc tttgcgctgc gcggtcgttt cgaaatgcac aacctgcccg gtaccgtgta
    27481 caccggcgtc agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga
    27541 gcgccttttc cagcttctcc tgcacactgc ggacttcatt ctgccgttgc cagccgtaat
    27601 acctactgcc gtcgtactca atgcccagcg caattttata aactggcggt tgttgctggt
    27661 cggacattag tacagatact cctgcaccag tttctcggca atttttactg ccatcagcgc
    27721 gccgccaaag cgaacgttat cggccaccga ccagaactga acttgctccg gcataccgta
    27781 gtcattacgt acgcagccaa cagaaagatg cggcgtacca gaagcatccc ctacctgagt
    27841 cgggaattcg ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc
    27901 ttcttctgcc gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccgtagaa
    27961 taccggtgcc tggacgacgc ttgccgaaat catcagccct tcgtcctgca ggattttgcg
    28021 tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg gcagtaacgg
    28081 cagcatgttg aacgccagct gacgcccgaa gaaatcttct tcgtcaatcg gaatgccatt
    28141 gagtaattta gcactctgcc ccgctaacgc atcgaccgct tttttgccct gggcggaggc
    28201 tgaaatcagg ctggtaacgc tgatacgtga taaaccgccc tgatcaatta gcggtttcag
    28261 tgccgccagc agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta
    28321 atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc
    28381 aaacaatccg ctgctgtcga tcaccagaca acctgagttg gtcgcttctt caacccaggc
    28441 agcggtagcc tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc
    28501 agcagcatcc tgcacggtga ttgtcttacc accaaagcgc agttgttcgc ctgcgctttc
    28561 gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag
    28621 cagggcttcg cccacagcgc cagttgcgcc caggacggca atgttccagc cttcagacat
    28681 ggtggtttac tccagaaata gcaaagctcc ctgccaatat tacagcagag agcatgaaga
    28741 agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc
    28801 gcgctggcgt catcacaaat cacatacaga gatgaccatt cacggcgctc aagatagttt
    28861 ttgcgcagct tatcgaactc acccggtatt ccggcgactt tacgcagcgg tgcgtcatcg
    28921 cggcgcacat catacaccaa atgcaccagc ctttttagcg tcggttgatc gagcggacca
    28981 tgcagcgtaa tgcggccaaa ctctggcgcg ggcagcaacg tatccagcgc aacgtgttgt
    29041 tcatgcccaa taagcttgct ataggcttca aacacttgcg tagtaccgcg tgctttacct
    29101 tccagggtat agcctgcgat atgcggtgtg ccgatatcca cttttttcag caattccacg
    29161 ttgagttccg gttcgccttc ccagacatcc agcactacgc ttaacttctg gccttcgttc
    29221 agacaggtca atagggcagt attatcgacg actgcgccac ggcaggcgtt aatcagaatc
    29281 gctcctggtt tcaggctacg gatcagtttt tcatccgcca gatgcagcgt tttgtacggc
    29341 ccgtctttga agagtggcgt atggaaagtc agaatatccg catgctgaac taattcatcc
    29401 agcgagcgga aatccccctc atccccgcgg tcggcgcgag gcggatcgca aagcaaggtt
    29461 gtaatcccta acgcttccag tcgcgcctgt aaacggcgac caacgttacc aacgcccacg
    29521 atccccactg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagtgag
    29581 gagaaaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt
    29641 cccgcctgct ttaaccatgc ttcatcgaca tggtcggtcc ccgcggtggc agtgccaaca
    29701 aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccata
    29761 agcgcatccg cgtctgccag ttgagcgaca ggaattggac gcccgggaac cgcggtcacc
    29821 tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc
    29881 acgtttgtgt tacctgtatg agacgagagt taaccagaca agtgtgccat aatctcgcgg
    29941 ccaggcatac ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg
    30001 ccctcctcct gggcaaccac caggccaggg agataacctg ccgtctggcg cgggcaatca
    30061 gcctttatcc agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact
    30121 gacgcaacaa caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg
    30181 agattcaccg ctgctttcgc gtcacttccc tgccgctgag cacaatctgg cgcaacgtct
    30241 gctggccgcg caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt
    30301 acgtctgggg aataatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac
    30361 gccgctgaat cagctctcac cggagcaatt aaagaccatt ctcaccctgt tgcaggaagg
    30421 gaagatggtt attccgcaac ctcagcagcg cgaggcgacc gaccgtccgt tattaccggc
    30481 ggagcacaat gcgctcaaac agctggtgac caaacttgcg gcggcaacgg gagaacccag
    30541 caagcagatc tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc
    30601 agcgaaactg tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca
    30661 aaatacgccg acgctggaat cactccagat ggcgctaaaa caacctttag atgccagtga
    30721 actggcggcg ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct
    30781 ttcttctgcc caggccgagg atattcttaa tcagctttat caacggcggg ttaaagggat
    30841 tgagccgcgt gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca
    30901 aaatatggca acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt
    30961 ctggctggtt cgttagcccc gacgaaatga cagtatcgtg acaataattc ccagcaccgc
    31021 agagatcgcc ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc
    31081 cagcggcccg gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt
    31141 gccgcgaact tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag
    31201 cgaacatccg gctccggtta acgccgcgcc cgctaatgcg acccacgcac caggggcttg
    31261 ccagagcagc aacaagccca ccgtttctac aagcagagag acgatcgcca ctttcacgcc
    31321 gccaaaacgg tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa
    31381 cgcggtaaga gtaaagcccg ccatcgccca tcctttactg gcaaagtaga gcgaaacgaa
    31441 agtcccgata accgcaaaac caacgccttg tagtgccaga cctaaccctg gtttccagat
    31501 aagcccgaca acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac
    31561 tgtgccgtta caggcccacg ccagtaaggg taatgccatt gtggtgagcg ccagtgcggc
    31621 aaaaccgtaa tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta
    31681 aatcgccatt ccgttccatg acatcacttt gccggaatgt tttggcccaa cgatgcctaa
    31741 tccccaggtc agagcgcccg tcagtaactg gctttcacca aagccaagaa tcaaacgccc
    31801 gacgaccaac agggcaaatt tgaacggtgc ggagacgggc aaaatcgccg ccaacagcag
    31861 cgcgccgcca gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata
    31921 ttgatcggcc agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatgcc
    31981 gacggcaata ccgaccatgg tgttgccata gcccagttca tgatgaacaa acagcgggat
    32041 aaccggcaac ggcagcccta cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa
    32101 gagcgaaaaa ttggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc
    32161 cagaatgcag cgcaaggcga ggaatttccc cgtctcatct ctctggtttc agggttacgg
    32221 tgcgttggca ggatttaacg cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt
    32281 tgcctggaac aggcggcgat tgtcaataat gtgaaaaagg gaaccatcag gttccctttt
    32341 gcgttagtgt cggatgccgc attggcgcgt aatgtcggat gcgacgctgg cgcgtcttat
    32401 ccgacctact ccgaattaat ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc
    32461 gaagccgaag ctgttagaca taactgtggt cagttcgcga tcggtcgttt cggtcacgat
    32521 gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaaacc
    32581 gtgttccagc atcagcagag agtagatagc ttcctgtacg ccagcagcgc ccagagagtg
    32641 accggtcatt gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat
    32701 agctgccagt tctttcacgt cgccaaccgg agtcgaagta ccatgagagt tcaggtagtc
    32761 gattggggta tcaacaccgt gcatcgccat cttcatgcag cgcactgcac cttcgccaga
    32821 cggagcaacc atgtctgcgc catcagaggt tgcgccgtag ccaacgattt cagcatagat
    32881 gtgagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccaccgcc
    32941 agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc
    33001 gttgtattta gtagacagcg cacccatcgc gtcgaattcg caagccattt cccagcacag
    33061 ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc
    33121 gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccgtg
    33181 aattttaaac ggcgtggcga ggcaggcaga aacgccggat gccatcgctt tggtgaccac
    33241 atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaaaacctg
    33301 gaaacgcggg gagccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta
    33361 agcttccggg gagaggccag catcagcaat tgcctgctcc atagaaagga atgcataaat
    33421 ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt
    33481 tacgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat
    33541 ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga
    33601 aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat
    33661 agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca
    33721 attaatagag aaatttgcgc agcctgacac acatcgctaa gatcgagcca ccgcctgtaa
    33781 gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg
    33841 agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat aacggactgg
    33901 aagagacgcg ttatgttttt ctcgggggta accatttaga ggcacgtttt cctgaacatc
    33961 cacatcctct gtttgtggta gcagagagcg gcttcggcac cggattaaac ttcctgacgc
    34021 tatggcaggc atttgatcag tttcgcgaag catatccgca agcgcaatta caacgcttac
    34081 atttcattag ttttgagaaa tttcccctcg cccgtgcgga tttagcgctg gcgcatcaac
    34141 actggccgga gctggccccg tgggcggaac agcttcaggc gcaatggcct ttgcccttgc
    34201 ccggttgcca tcgtttattg ctcgatgaag gccgcataac gctggattta tggtttggcg
    34261 atataaacga actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt
    34321 ttctggacgg ctttgcgcca gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg
    34381 ccatggcccg gctggcgcgt ccgggcggca cgctggcgac ctttacgtct gccggttttg
    34441 tccgccgcgg tttgcaggaa gccggattca ctatgcaaaa acgtaagggc tttgggcgca
    34501 aacgggaaat gctttgtggg gtgatggaac tgacattacc cctcccctgc tccacgccgt
    34561 ggtttaaccg tacgggcagc agcaaacggg aagcggcgat tatcggcggt ggtattgcca
    34621 gcgcgttgtt gtcgctggcg ctattgcgtc gcggctggca ggtaacactt tattgcgcgg
    34681 atgaggcccc cgcactgggt gcttccggca atcgccaggg ggcgctgtat ccgcttttaa
    34741 gtaaacacga tgaagcgcta aaccgttttt tctctaatgc gtttactttt gctcgtcggt
    34801 tttacgattt attgcccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct
    34861 gggatgagaa aagccagcat aaaatcgcac agatgttgtc gatggattta cccacagaac
    34921 tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca
    34981 gcggtattac ttatccgcaa ggtggctggc tgtgtccagc agaactgacc cgtaatgtgc
    35041 tggaactggc gcaacagcag ggtttgcaga ttcattatca atatcagtta caggatttat
    35101 cccgtaagga tgacggttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagtt
    35161 tagtggtact ggcgaacggg catcaaatca gccgtttcag ccaaacgtca tctctgccag
    35221 tatattcggt ggccggtcaa gtcagccata ttccgacaac gccggagctg gcgaaactga
    35281 agcaagtgct gtgctatgac ggttatctca cgccgcaaaa tccggcgaat cagcaccatt
    35341 gtattggtgc cagttatcat cgcggcagcg aagagacggc gtacagtgat gaagatcagc
    35401 agcagaatcg ccagcggttg attgattgtt tcccgcaggt acagtgggca aaagaggttg
    35461 atgtcagtgg taaagaggcg cgctgcggtg tgcgttgtgc tactcgcgat catctgccaa
    35521 tggtaggcaa cgtgcctgat tatgacgcta cactcgtgga atatgcgtcg ctggcggaga
    35581 agaaagatga agcggtaagc gcgccggttt atgacgatct ctttatgttt gctgctttag
    35641 gttcgcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggca cagatgagtg
    35701 aagaaccgat tccgatggat gccagcacgc tggcggcgtt aaacccgaat cggttatggg
    35761 tgcggaagtt gttgaagggt aaagcggtta aggcggggta atctgctctg gcattgtttg
    35821 tcggatgcgg cgtaaacgcc ttatccgacc tacgcgttac ctgtaggcct gataagacgc
    35881 gataagcgtc gcatcaggca tggtgctcca gacgtcacaa cttactgctg tgaggcctgc
    35941 tgaaacaggt gttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg
    36001 gcaccaatgg ctttttccag actctggcta acggttgtat gtaccgcctg agcggagtgg
    36061 tcgtcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg
    36121 gcaaacaact catcatcact ggcatggtca accataccgt cgattaatgc cagaatgcgt
    36181 gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg
    36241 agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt
    36301 agtgctttaa tgaagcgtgc cggacgttcg ggaatgccgt ttgccagata atccatcacc
    36361 tgcgcatgta cccgccgttg aaacaccacg cggtccggtt cgaaatcccc ttccagattg
    36421 tcgcagctaa cattaaacgg atatcccgct gccacgcaga acaaccagtc cagcgcctgc
    36481 ggcttcactt caacatcttc aaactggctt tgtgtctggg catcgcgtcc gtccgggcaa
    36541 taccagtagc cgaaatcaac cagctcacgg cgcgctttcc cggcaataca ccagtgcgaa
    36601 atctcgtgga tggcgctggc ataaaagcca tgagcgaaga cgattctgtt atacggtact
    36661 tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat
    36721 tcatcggcaa agcagctatt aaaaatctca atcaactgct catagtggtg tgtactgttc
    36781 attagttcat ccccaaccag tggaggatct cctgtccatg gctatcgtaa agtaatttgg
    36841 cactcatcac cgccgagacg ataacgatca tcgggcggat cagcttttgt cctttgctca
    36901 acaccagtct tgaacccatg cgcgcgccga ggaactgccc caccagcatc acaaagcccg
    36961 tcgcccaaat cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg
    37021 cgttaagtaa tttggcgtga gcagtggctt tggcgaggtt gaatccgcac agcgtaacga
    37081 aggccagcgc gtaaaacgat ccggcagccg ggccaaaaaa tccatcgtaa aaaccgacgc
    37141 agccccccgc caccagcgca aatggcagtc catacatccg acgctggcga tcttcttcac
    37201 ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaata ggcaaaatct
    37261 gccgtaagac atcggcctga acatactgca ccagtagcgc gccgctcatt gagccgacaa
    37321 aggtcatggc gatattgagt ttttgatcgc ttaaactaac cactttacgg cgaataaagt
    37381 agatagtggc ggaaatagag ccaccgcagg cttgcagttt attggttgcc agcgcgttag
    37441 cgggagacat ccccgccgcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa
    37501 tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc agcagcgggg
    37561 aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg
    37621 gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg
    37681 aggttcaaac cagctttgca gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg
    37741 atcctcacac tccagactat cggcaggaca acgtaatcgt acatgcatat gcgcgcgatg
    37801 ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag
    37861 ttgttgctta atcgccggat taacaaaaat gcgcgtgacg tctttatcct gggcggcgag
    37921 tttgatcaag ctgaaaattt ctggcttcca cagcgtcggg acaacgtgtt taccgtcgcg
    37981 ggaaactaaa tctaacgctt gcgggcgcaa gagttgcgcg gaggtccagc gtgttttcgg
    38041 cagttgcagg aagatatcga catccagtcc ggtctggtgg ctggcgtgac cgccgttgaa
    38101 acgaccacca gcaggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct
    38161 cacctggcga ctcagacgct ggataaacat caccaggtct gggtgaccga aatagcgacg
    38221 ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcaccgat
    38281 gatacagcca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat
    38341 tttttgccac ggcgtcgctg ccaggctggc gctactggca agcagagcca gcagcgcaat
    38401 cgcggttttg ttcatttttt accagcgtgg aatttcagtc ttcacatcgg cattttgcgc
    38461 ccgctgccgt aacaggtgat ccatcaaaac gatcgccagc atcgcttctg cgatcggcac
    38521 cgcgcggatc ccaacacaag gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc
    38581 aaagcggtta atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatgtg
    38641 ggcaatgatt tgctgcccgc tgctgatacc accaagaatg ccgcccgcat ggttgctctg
    38701 gaaaccgtct ttggtgattt cgtcgcggtt ctggctgcca cgcagcgcta ccacgtcaaa
    38761 accatcacca atttccacgc ctttcaccgc gttaatgctc attagcgcat gggcgatgtc
    38821 ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg ggtacgccac tggcaacgac
    38881 ggtgactttc gcaccaatag agtcgccttc ttttttcagc gcgcgcatca gttcatctaa
    38941 cgcgtcgatt ttgtccgggt ccgggcagaa gaatggattt tgctcgacct gagaccagtc
    39001 tttgatttcc agcggaatgt cgcccatctg ggtcaggcaa ccgcgaatct caatgccaaa
    39061 tttctcggcg agatattttt tggcaatcgc ccctgctgca acgcgcatag cggtttcacg
    39121 ggcggaagaa cgtccaccgc cgcgataatc acgcaggccg tatttttgtt cgtaagtgta
    39181 atcggcatga cctggacgga aaacgtcctt aatcgcacta taatcctgag aacgctggtc
    39241 ggtattttcg atcaacaatc cgatgctggt gccagtagta acaccttcaa aaacaccgga
    39301 gagaattttg acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg
    39361 acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc
    39421 atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag
    39481 ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt
    39541 aatctttata aatcgcgaaa tgttctcgtg cggcaataag ctgctcttta gtgagcataa
    39601 acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcc ggatattgtt
    39661 ccataagatg taccatgctg ttgccgactt cacaaatcag cacgccatca tcagcgaggt
    39721 aatctgccgc gtttccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca
    39781 ggcccagttc cggctcgtgg cgatattcgt ttggcaggtc ggacatatct tccgcatcga
    39841 catacggcgg gttagtgaca atcaggtcgt actgcacttt cggcaagtcg cggaacaaat
    39901 cggaccgaat aggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcagcaa
    39961 ccgccagcgc gtcgggagag atgtccaccg cgtcgacttc cgcttccggg aaggcataag
    40021 cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatgtgc tgcggttgct
    40081 tgctgatgag tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca
    40141 gcacgcgttc atcgacgtaa aattcatggc cgcagaacca ggctctgttg gtcaggtaag
    40201 ccaccggaat gcgttcgttg acgcggcgga tcacgcgctc aacaatacgg tgtttttcgc
    40261 tggaggtcag acgcgcggtg cgcatgtctt ccggaatatc cagcggcagg tagagtgaag
    40321 gcaacaccag ttgcacggct tcatcccacg ggttatcggt gccgtggccg taccagatat
    40381 tcgccgcgct gaagcggctc accgaccagc gcaacatgtc ctgaatggtt tgcagctcat
    40441 ttactgcttc atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat
    40501 ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag
    40561 gaaaaatacg tttaaaacga tcgattgcgc tacgagtcgg gtaaactgta ggaaaattag
    40621 aaatagagaa agataaatga aaaagaaaac aacacttagc gaggaggacc aggcgctgtt
    40681 tcgccagttg atggcgggta ctcgcaagat taagcaggac acgattgtcc accgaccgca
    40741 gcgtaaaaaa atcagcgaag taccagtaaa gcggttgatt caggagcagg ccgatgccag
    40801 ccactatttt tcggacgagt ttcaaccatt attaaatact gaaggtccgg tgaaatatgt
    40861 tcgcccggat gtcagccatt ttgaggcgaa gaagttacgc cgtggcgatt attcaccgga
    40921 gttgtttctt gatttgcacg gtctgacgca gctacaagcc aaacaggaac tgggggcgtt
    40981 gattgccgcc tgccgccgtg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa
    41041 gcatattttg aagcagtaaa cgccgctgtg gctggcgcaa catccacatg tgatggcatt
    41101 tcaccaggca ccaaaagagt atggtggtga tgcggctttg ttggtgttga ttgaagtgga
    41161 agagtggctg ccgccggaat tgccctaata aatattgcgg tataaacgtc ttctccgacc
    41221 aggatactga ccggagaaga attaacccta caactcattc aaaatgtact ggaaacgaca
    41281 ggtggcatcg attggctgct gactgctggt gtcgatccgt atttgcatat catgcagttg
    41341 cttatcggta attggatgtg ctaccggcgt tactgtgact ttgctgtcgt cacaccccga
    41401 cgtgctgaat gtgatcatat acgacataaa gcgacaataa gcgccacgat cgtccattcg
    41461 gcttccatca cacacccgcc cgtcgcgcat caggtaagtc gagagcgttt gtgttatgac
    41521 tccccccgtc gattgcagcg ttagcatctt gttatggagg acgtagttct cattccagga
    41581 gatatcatag gggccaaccg gctccgttgg gcaccgggtt tgttttgctg atggaccaat
    41641 gtaagtataa cctctcgcag cagtactcac cgtgttggta ctgaccaacc acgggcaccc
    41701 gtgagtttca ccatgcacga tgatatcgac ccccagatca acacatgacc atccatccgt
    41761 aatccctggg caattggagt tatccacagt attactctga tgagtgatgt tcatcgttgg
    41821 tgctgatccc ggtcccgtcc aaaccgtgtt agggatattc cccaaccaca acgtatatcc
    41881 atcctgtgca tattcgccat aatagacccc ttccggcgtc aagacatcga tacgaaagac
    41941 aacttttgtt gtagccccgt taccccaaaa tccggtataa gtcttcacat ccctgaatgt
    42001 gacgtccgcc caggcggttg cactcagtag cccggtcagc agtaaaaaaa acagtccgct
    42061 catttttttc atgttaaata tactcaaacg tcgcagtgag ggaggccgta aagtcaccca
    42121 tcgtcacatc ccgcccgctt tttgcctgta gccaggcatt aaaatgcaga tcattgttgc
    42181 cgctgctcag cacaaatgtc gccccgctgg tattgttgat tagcacagca gtgccgtcgg
    42241 tcttttccat gccaatcccc acaccttgtg ctgtcgaagc ggcatccagc gccagaaaac
    42301 cgggctgctc gctgtcttcc gttccggtaa gtgtcacccg aacctcatac tgagcaggcc
    42361 ctttgcaatc tttaagctga aacaccaccg gtacgcgcgc agattgcccg gtcaccatca
    42421 gatctctacg gctgaccgtg gggaaatgca cttccgccag cacctcgccc tgaaccacca
    42481 gcgtgcagga cttactgagt aaattgccgt agaaatgcag gttgtctgcc gcctgaacat
    42541 tcggcgtcag cagcaccgcg gccagcgcag ccgcgcagtg aaatatccgt tttaggctca
    42601 ttggtaatcc acctgtaatg ttgcccaggc agaaaaggtt ccttcgctga gtgaagcatc
    42661 actggctttc accggtacag cttgcagcgt tggcggcgtt gacaggttga ttttcagcgg
    42721 cgtacccaac gtgaacggct ggccattttg ttgcagctca atccccagcc cggtgacgtt
    42781 tgtctcaata gccgcgttat cgtagctggt ctggctgccc gtccagctca gcgtcatctc
    42841 ccaggcatcg tcgcgcacgt cgggatcgca tgtaatggta tagggcacat tctggcggaa
    42901 gttattgccg ttgatgttgt cgataatgac gtcacgaaac tcaacttcaa cggttttccc
    42961 gtcactgatg gcacaggcgg gcggcgaaac caatgtgcca tgaaatgtga tatcgctatc
    43021 ggccgcctgt gcaataccgc aataacaacc gaataagcag agcgcaaatc gtttcattga
    43081 tactccacca ccattgtcat ggttgcgcta aactctgatg ccgtaagctg tgtgccgctt
    43141 tgtttcaccg gcacggcttc gaaacccggg cgcgaattaa cgttaaacgg cagccagtta
    43201 ttacccgcca acgcaaccgg cgcatggctg gacgcatcct gtatgcgaat gccaaaacca
    43261 ttgatgcccg tactgaggac cgtttcaccg ttaatggtgg tggtggtccc ctgtaactgc
    43321 atctgtaggt catcgctgac gctgttcgaa cagttcaggg tgtaaccaac aggctgccga
    43381 tagttgctgc cgtcgatgtt tttaataatg acattgccaa actccaccgc gctgcctttt
    43441 accgtacagg gcggcggtgc atccaccagt atacgaagcg ttaagttgac cgttttacta
    43501 tcggcccagg cggagccgca ggccagtaac accatgccag cgacgggtaa taaaactcct
    43561 ttcatgctgg atctcctgtc attagccttt taggctttga gcttcatcca ccttgcaggt
    43621 gtctccttca caacggaaga ccagcggtaa acgtgcgccg tagtcgttga tgtaggtcag
    43681 caccggagcg ctgttcattt ttgcctttaa cggttgtgac gttttcggcg gcatcaccag
    43741 tggtttaaag cccgcagcag ttagcccctt tttctgcgta ctggcatcgc tgatgatgac
    43801 gtggtacggc gtcgggttat tgacgatata gtcctggccg ctgcgcgtga gtgtgacctt
    43861 aaactgccac ggttttctca tgtcaatctt ctccagcgcc tttggtcgcc agaacagctt
    43921 gatgcgggtt tgcagcgcaa tttgcagggt gttggctttc ccggactttg gcgggatctc
    43981 acgcacattg tagtagaaca ggctttcacg gtcagacggc agcgtgtgaa tgtccggcag
    44041 cgcctgaatc ttcacctgac cgttcatcat ggcgtcaata cgctgcaccg gcggcagtac
    44101 cgccagcggc gaagtgattt tgttgccctt ctcgtcttcc agccagcttt gcgccagata
    44161 aggcagcttt tcgttattgt tgcgcagggt tacgcttatc gatttatcac tttcgttaaa
    44221 caccagacgg gtgcgatccg gagtgactga tgcgtgcgcc gccaggttgg tggtggtcaa
    44281 cagtaccgcc agcgtcgtgc tgaccaccat tgaacaggat aatttataaa acattgattg
    44341 ttccttaatc atattattga gtggctgaaa ttgacgtcgg cgcttcagat ggcgtgacct
    44401 gccgagtctg gtcctgaata acgggcttaa tttccggcgc taaagaatcg ggggctgtcg
    44461 ttcccttttg ttcacacggc aacaacaggc cgcggaacag atcggcaggt agcggcttcg
    44521 gcaatacgat ctcgcattgt gcgctgcctt cccagaacac catcatatgc tcacccgcat
    44581 tgatccccgc cagatagaca ttgccttcat catccacaat gccaacctgc tgctgctcgt
    44641 cgttttttac ttccgcgcca aacggcgggt agctaccgtc acgcaaccgc agtaccgcca
    44701 tcgctttttg cccgctgatc actttgaatt tacgatagcc gattgcgcct tccgtcagag
    44761 tcgcctgcac caccgactgg gtggcttccg catcttccgg caagttgttt aaatctatat
    44821 acgcctgatt gcgatagtag ttgttaatat cggcgaccac cgccttgcca aacatattgg
    44881 tgtacaccgg cgcgccgttg ctttcgaccg gcacgttggc aataccgtcg gcgtcaatca
    44941 gcaggcgtgt gccgcccatg ttttgagtgc gatgcagcgc cccaccgtgc gcggtcagcg
    45001 ttgccccgcc ctgtaaggcg atccccgccg aacggtattc tccttcatgg tagttggcgc
    45061 tgagatctac cttcgccaac gtgccgtcgt ggctcagata accatccacg ctaccgtgct
    45121 gttcgctggt gccgacgtta atctggtagt gcgttgcgtc atcgatgcga ttaaagtagc
    45181 cgacctgact gctgtccgac ccgctaccgt aagagccgtt gtaggtcacg gtgctgctgt
    45241 cgctccacgg aatgctcatg ctgaggtaca ttcccttatc cgtattatcg tcatattcgt
    45301 agcgatagcc ggtcaccgag atgctcatat tgcgaatgct ccccatatta aaatagtggg
    45361 aaaacatcag gttatagttt gtctgttccg ggcgatccca gtaagtacga tgggagtagt
    45421 tgagatagac cgagacaccc gctgcggcaa agttctggtt atacgtgatc gtatacatct
    45481 ctttgtcgtt tccggtccgc gccatgtccg actggctcgc gtccagatac tcgctcatag
    45541 tcataaagtt cttttcggag aaacggtagc ctgcaaaggt gacgcggctg ttaagttcgt
    45601 caaagtcttt ggcatagctc acgcgaaagg agttgccgtc cagttttcct ttgccgtatg
    45661 cgctgtcatg atccaggttg acgtgcgagt gagtcacatc gaacgccagc gcgccaaact
    45721 gtgcgaggtc gcgccccacc cccatcgccg ccgactggta gtgctcatct gccagcgcgc
    45781 caccgtaaag cgaccagcca tctgccaccc cccatgatgc ttctccgccg gaaaaaaatc
    45841 cgccttcggt cttgtggttc cagtcttccg gacgccccat catcactttg taacgcacct
    45901 gcccctgacg cgtgaggaat ggcatggatg cggtggtgac gtcatattcc tgcacctgac
    45961 cattctgttc ttcaacacgg acgtgtagtg tgccagagac agaatcgccg atatcctgaa
    46021 tgcggaatgg cccggcggga acctgggttt cgtaaagtac ccggcccatc tggctgatgg
    46081 tcacttttgc gctgctgtgc gccacgccag aaacatctgg cgcatagccg cgcagattgg
    46141 gcggcagcat ctggtcatcg gtgctgacac tgctgccgat atagttaaag ccgtcgaaaa
    46201 tatcggaatt gagataatct tctcccagcg acagcttcgc ttttaaagag ggtaaagctc
    46261 gccaggcgta ataacggctc cagtcccagt ttttactcgt tgtactgtta ctgctgtcat
    46321 cttcatcgtc gttgctgcgg gtgtgctgat aatcactttg ccagtctgca cggaaacgcc
    46381 atgccccaag gtttgcccca actgtaccgt taccgctgat atcatgtgag tcctcgcccc
    46441 cttgctcctg atggcgagtt tgcgcattga ggctgtagtc ggcaatcagc ccagaaatac
    46501 catcgtccca gcgtgaggga gggtcccagt cgctgctggt atattcgagg taagcctgtg
    46561 gcactgtcag cagcaaagcc gactggctta aatcattctc gacttccatc ccgtctaatt
    46621 gccccggttt aaggcattca tcgttgtgcg tccactgcag gctttttgct atcccttctt
    46681 tcagccccag cgccgccact aactcaggtg tcaggcaggc ataagttttt gttggatcgt
    46741 tttcagagac ataccaatta atgtcgtatt catcagaaag ggattgttta tttataaata
    46801 cacgtaaatt atatttaccc gcgtcgacat accctttttt tgaaaactta ccgagatcaa
    46861 ttttcgtgtc gccctttaac tcaaggaaac gggggtcaaa ttgaatggaa tcatcagccc
    46921 atgcattgac tgatccacca gagattgcca gcgcaatata gcaggcgatt ccccgcagcc
    46981 gaaaatttga gtgattaggc atacacatat ccgtttgtga aacagtcgta aatccgttgt
    47041 gcgttgaata accccacagc agaacgctgt ggggttgtca catcagagat aggtaatctg
    47101 gaatgtggtc agggtttcga actggccgag atccggcgca tcggtttcac ccaccagcca
    47161 ggctttaaag ttcagcgttt gcttgtcttt acccttgcta gttgaagcat ccatcacgat
    47221 tggctgaaca atcttttcgc ccactttata acctttgcca tgggtatcgc cgattaccag
    47281 gctcacgtgg ttcatggcaa caccgctgtc aacattgaat aacgcatagt tatctgcgtt
    47341 ggctggcccc gcgtagttag tgccggtgaa cgtggtctcc atgtttgatt cagtggtaaa
    47401 cacgcaatcc tgtaaatgga tctggaaatc tttcggtgtg gtagtgccgc cgccagtcag
    47461 tttagccgca ccgatatcgc ccatatcgac ttccagttta tgatcgtctg gaacgatgga
    47521 gcacggggag tcttcaatag tgccgtaaaa acgtgcggta ccgttgttac ctgcggcaaa
    47581 agcagaactg gagacgactg cgcccatgac cattgcggca gcaatggctg ttttggcaaa
    47641 cttactcatt att
//
LOCUS       DACADA010000018        45262 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.018, whole genome shotgun sequence.
ACCESSION   DACADA010000018 DACADA010000000
VERSION     DACADA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.018"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..5046
                     /locus_tag="ISB17_001018"
     CDS             <1..5046
                     /locus_tag="ISB17_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HAP1978977.1"
                     /translation="DGDNAISNGGTGTQINGDDATANNSGKTIVDGKDSTGTEIAGNN
                     AVVNQDGTLDVSGGGHGIDITGDSAKVDNKGGMTVTDPDSIGIQIDGDKAIVNNDGDN
                     AISNGGTGTQINGDEATVNNNGNTTVDGKDATGTEIAGNNAVVNQDGTLDVSGGAHGI
                     DITGDGAIANTTGAITVADTDSVGIQVDGNAVTVSNDGDSTITNGGTGTQVNGNDAQV
                     NNTGNTTVDGKNSTGTEITGNNGTVNLDGGLIVTGGAYGVEIAGDSAALNNEGDISVS
                     DEGSIGVLINGEKATVSNTGDVNVSNNATGFDITTNEGNISLAGSMQVGDFSTGMDLN
                     GNNNSVTLAAKDLNVTGQKATGVNVSGDANTINITGNVLVDKDQTADNAADYFYDPSV
                     GINVNGSDNNVTLDGKLTVVADSELTTRGSAGAIIFDGSQENITGLSIAGDGNTFRLN
                     GGIQLVGEANKLTDGSTIASERKGSGKVPLVSVDGKSSVHLNGDSTISGDVLLGNTQI
                     IDLRNGAMLDVGADATINMQVDTYERYAMSADHLIDASSGSQLVNNGDINLRSIGFAA
                     ITGEDSFGSNTGNLTLSQYLDGLFANGGYGYLATKGASVVNTGTITTKVAEQESVINL
                     GASLGFNDLVFYNAANSMTGLSAKTHGYVLNENGGKIEMFGRGNVGMLAIDESTAENA
                     GQITLDALWVDVNDTTTLRDNIGNDARGYSVGMGVGTDAGSGPRKNATAVNQQSGVIT
                     VYNAGIGMAAYGAGNTVINQGTINLEKNGNYDSSLGVNSLIGMAAYNSGTAINDQTGV
                     ININADNGQAFYSTGNGVILNYGTICVNTNCLTGNDYNDTDSYTSILYTGGDVITAQS
                     ETQSLTKKATISDKKDGNVVNSGALSGADIAISSGKLVNTSTGTINNAIIINGGALSN
                     EGSVAKVTQNAGTFNNTGSVNSRMTQTGGTFNNQQGGTVKNGSRLANSAIANNMGIWS
                     LGDTSSGSNASMLEINNNAVFKNSGDFILDNSKNAVHLNQSGTFYNTGKMLLSNSSHS
                     GVLNFWGGNGRFINGGIADVTAKSLAVSASDAGSSNAFFWNQSNGVVNFDKDSGVAVK
                     FIHSNYVAQNDGTMNISGNNAIAMEGDKNARLVNNGTINLGTSGTTDTGMIGMQLDSG
                     TTADAVIENNGAINIYANNSFAFSMLGSVGHLVNNGTVTIADGVTGSGLIKQGDSVNI
                     EGVNGNNGNNSEVHYADYTLPDVPGSSVSVAASTPSSDGSQNKLNGYVVGTSSDGSAG
                     KLKVNNASLKGVSVNTGFTAGTSSTSVTFDNVVQGSNLTDAETITSTSVVWNAQGTTD
                     ASGNVDVTMTKNAYTDVATDSSVNTVAQVLDAGYTNNELYSSLNVGTTAELNSALKQV
                     SGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNDTQYDMMAL
                     RKSLTLTEHQNLSLEYGIARLEGNGSDTAGDNGVTGGYSQFFGLKHQMAFDNGMNWNN
                     ALRYDVHQLDSSRSVAYGDINKTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVK
                     LRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKDGWSANATLEGGPNLS
                     YVKSQRTASVSGAGSQRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAFHWKEDGI
                     SDKGVMLNFKKTF"
     gene            5266..6132
                     /gene="pdxI"
                     /locus_tag="ISB17_001019"
     CDS             5266..6132
                     /gene="pdxI"
                     /locus_tag="ISB17_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HAP1978978.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(6172..6777)
                     /gene="azoR"
                     /locus_tag="ISB17_001020"
     CDS             complement(6172..6777)
                     /gene="azoR"
                     /locus_tag="ISB17_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP1978979.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6978..10880
                     /gene="hrpA"
                     /locus_tag="ISB17_001021"
     CDS             6978..10880
                     /gene="hrpA"
                     /locus_tag="ISB17_001021"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAP1978980.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            11153..11953
                     /locus_tag="ISB17_001022"
     CDS             11153..11953
                     /locus_tag="ISB17_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP1978981.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            12163..13602
                     /gene="aldA"
                     /locus_tag="ISB17_001023"
     CDS             12163..13602
                     /gene="aldA"
                     /locus_tag="ISB17_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP1978982.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13644..14645)
                     /gene="gap"
                     /locus_tag="ISB17_001024"
     CDS             complement(13644..14645)
                     /gene="gap"
                     /locus_tag="ISB17_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP1978983.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14834..15364
                     /gene="cybB"
                     /locus_tag="ISB17_001025"
     CDS             14834..15364
                     /gene="cybB"
                     /locus_tag="ISB17_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAP1978984.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15609..15782
                     /locus_tag="ISB17_001026"
     CDS             15609..15782
                     /locus_tag="ISB17_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1978985.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(15854..16003)
                     /gene="hokB"
                     /locus_tag="ISB17_001027"
     CDS             complement(15854..16003)
                     /gene="hokB"
                     /locus_tag="ISB17_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAP1978986.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16266..16709
                     /gene="trg"
                     /locus_tag="ISB17_001028"
                     /pseudo
     CDS             16266..16709
                     /gene="trg"
                     /locus_tag="ISB17_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(16747..17670)
                     /locus_tag="ISB17_001029"
     CDS             complement(16747..17670)
                     /locus_tag="ISB17_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1978987.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            17887..19230
                     /locus_tag="ISB17_001030"
     CDS             17887..19230
                     /locus_tag="ISB17_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1978988.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHKEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            19455..21110
                     /locus_tag="ISB17_001031"
     CDS             19455..21110
                     /locus_tag="ISB17_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAP1978989.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            21250..21474
                     /locus_tag="ISB17_001032"
     CDS             21250..21474
                     /locus_tag="ISB17_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAP1978990.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            21537..22076
                     /gene="rimL"
                     /locus_tag="ISB17_001033"
     CDS             21537..22076
                     /gene="rimL"
                     /locus_tag="ISB17_001033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAP1978991.1"
                     /translation="MTETIKVSESLELHAVAESHVTLLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(22068..23048)
                     /locus_tag="ISB17_001034"
     CDS             complement(22068..23048)
                     /locus_tag="ISB17_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAP1978992.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            23172..24164
                     /gene="tehA"
                     /locus_tag="ISB17_001035"
     CDS             23172..24164
                     /gene="tehA"
                     /locus_tag="ISB17_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAP1978993.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLCLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            24161..24754
                     /gene="tehB"
                     /locus_tag="ISB17_001036"
     CDS             24161..24754
                     /gene="tehB"
                     /locus_tag="ISB17_001036"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAP1978994.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            25057..25725
                     /locus_tag="ISB17_001037"
     CDS             25057..25725
                     /locus_tag="ISB17_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAP1978995.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(25760..26971)
                     /gene="ydcO"
                     /locus_tag="ISB17_001038"
     CDS             complement(25760..26971)
                     /gene="ydcO"
                     /locus_tag="ISB17_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HAP1978996.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            27024..27560
                     /locus_tag="ISB17_001039"
     CDS             27024..27560
                     /locus_tag="ISB17_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1978997.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            27633..29594
                     /gene="rlhA"
                     /locus_tag="ISB17_001040"
     CDS             27633..29594
                     /gene="rlhA"
                     /locus_tag="ISB17_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HAP1978998.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(29686..29916)
                     /locus_tag="ISB17_001041"
     CDS             complement(29686..29916)
                     /locus_tag="ISB17_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HAP1978999.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            30095..30331
                     /gene="hicB"
                     /locus_tag="ISB17_001042"
                     /pseudo
     CDS             30095..30331
                     /gene="hicB"
                     /locus_tag="ISB17_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            30410..31816
                     /locus_tag="ISB17_001043"
     CDS             30410..31816
                     /locus_tag="ISB17_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP1979000.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            32061..33206
                     /locus_tag="ISB17_001044"
     CDS             32061..33206
                     /locus_tag="ISB17_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1979001.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            33224..34237
                     /locus_tag="ISB17_001045"
     CDS             33224..34237
                     /locus_tag="ISB17_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1979002.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            34238..35179
                     /locus_tag="ISB17_001046"
     CDS             34238..35179
                     /locus_tag="ISB17_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1979003.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            35169..35963
                     /locus_tag="ISB17_001047"
     CDS             35169..35963
                     /locus_tag="ISB17_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1979004.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            35985..37409
                     /gene="patD"
                     /locus_tag="ISB17_001048"
     CDS             35985..37409
                     /gene="patD"
                     /locus_tag="ISB17_001048"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAP1979005.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(37506..37601)
                     /gene="yncL"
                     /locus_tag="ISB17_001049"
     CDS             complement(37506..37601)
                     /gene="yncL"
                     /locus_tag="ISB17_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAP1979006.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            37797..37970
                     /locus_tag="ISB17_001050"
     CDS             37797..37970
                     /locus_tag="ISB17_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAP1979007.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            38056..38289
                     /locus_tag="ISB17_001051"
     CDS             38056..38289
                     /locus_tag="ISB17_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAP1979008.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTL
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(38290..38739)
                     /locus_tag="ISB17_001052"
     CDS             complement(38290..38739)
                     /locus_tag="ISB17_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1979009.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(38736..39254)
                     /gene="mddA"
                     /locus_tag="ISB17_001053"
     CDS             complement(38736..39254)
                     /gene="mddA"
                     /locus_tag="ISB17_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAP1979010.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            39435..39503
                     /locus_tag="ISB17_001054"
                     /pseudo
     CDS             39435..39503
                     /locus_tag="ISB17_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase yncb"
     gene            39503..39658
                     /locus_tag="ISB17_001055"
                     /pseudo
     CDS             39503..39658
                     /locus_tag="ISB17_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39735..41837)
                     /locus_tag="ISB17_001056"
     CDS             complement(39735..41837)
                     /locus_tag="ISB17_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1979011.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            42079..43140
                     /locus_tag="ISB17_001057"
     CDS             42079..43140
                     /locus_tag="ISB17_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAP1979012.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(43253..44752)
                     /gene="ansP"
                     /locus_tag="ISB17_001058"
     CDS             complement(43253..44752)
                     /gene="ansP"
                     /locus_tag="ISB17_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAP1979013.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
BASE COUNT        11545 a        10950 c        11769 g        10998 t
ORIGIN      
        1 gacggcgaca acgccatcag caacggcggt accggcacac aaatcaacgg tgacgacgcg
       61 accgcgaaca acagcggcaa aaccattgtc gacggcaaag attccaccgg taccgaaatc
      121 gcgggcaaca atgcggtggt gaatcaggac ggcacgctgg atgtcagcgg cggcggtcac
      181 ggtattgata tcaccggcga cagcgccaaa gtggataaca aaggcggtat gacggttacc
      241 gatccggatt ccatcggtat ccagatcgac ggtgataaag ccatcgtcaa caacgatggc
      301 gacaatgcta tcagcaacgg cggcaccggc acgcaaatta acggcgatga agccaccgtg
      361 aataataacg gtaacaccac tgttgatggc aaagacgcca ccggcacgga aatcgcgggt
      421 aacaacgcgg tggtgaatca ggacggcacg ctggatgtca gcggcggtgc tcacgggatt
      481 gatatcactg gagacggcgc cattgcaaac acgacaggag cgatcactgt agcggatacg
      541 gactctgtcg gtattcaggt tgatggtaat gcagtgaccg tcagcaatga cggagacagc
      601 accattacta acggcggtac gggcacgcag gttaacggca acgatgctca ggtcaacaat
      661 actggcaaca ccacggtaga cggcaaaaac tccaccggaa cagaaattac gggcaataac
      721 ggcactgtaa atctggatgg tggtctgatt gtgacgggtg gtgcctatgg cgtggagatc
      781 gctggcgaca gcgccgcgct aaacaatgaa ggagatattt cggtttccga tgagggctca
      841 attggcgtac tcatcaacgg tgaaaaagca accgtatcca acacgggtga tgttaacgtc
      901 agcaataacg cgacaggttt tgacattacc actaatgaag ggaatatttc gctggcaggc
      961 agtatgcagg tgggcgactt ctctaccggg atggatctaa acggtaataa caacagcgtc
     1021 acgctggcgg caaaagatct gaacgtcacc ggtcagaagg cgacaggcgt gaatgtatcc
     1081 ggcgatgcca acactattaa tatcaccggt aatgttctgg tagataaaga tcaaacggct
     1141 gataacgcgg cagattattt ctacgacccg tctgtgggga ttaatgtcaa cggtagtgat
     1201 aacaatgtca ctctggacgg taagttaacg gttgttgctg acagtgaact tactacacgc
     1261 ggttctgctg gcgccattat atttgatggc agccaggaga atatcaccgg actcagcatt
     1321 gcaggtgatg gtaatacttt ccgtctgaat ggcggtattc aactggttgg tgaggcgaac
     1381 aagctaactg acggttccac tattgcatcc gagcggaaag gcagtggaaa ggtacctctt
     1441 gtcagtgttg atggaaaatc gtccgttcat ctgaacgggg attccactat tagcggtgat
     1501 gtgctgctcg gcaatacaca aatcattgat ttgcggaatg gtgccatgct ggacgtcggt
     1561 gcggatgcca cgatcaacat gcaggttgat acttacgagc gttatgctat gtcagccgat
     1621 cacctcattg atgcatcgtc tggctctcag ttggtcaata acggtgatat taatctccga
     1681 agcatcggtt ttgccgctat tactggtgag gattcattcg gaagcaacac tggcaatctc
     1741 acgctttctc agtacctcga tgggctgttt gcaaacggag ggtatggcta tttagccacc
     1801 aaaggggcca gtgtcgtgaa cacaggcact atcactacga aagtggcgga acaagagagc
     1861 gtaatcaacc ttggagcgtc actgggtttt aatgatttag tcttttataa cgcagcgaac
     1921 agtatgacgg gccttagcgc caaaactcat ggttatgtac ttaatgagaa cggcggcaaa
     1981 atcgagatgt tcggacgtgg caatgtcggt atgttggcca tcgatgaaag tacggcagaa
     2041 aacgcggggc agattacact ggatgcactg tgggttgatg taaacgatac aacaactttg
     2101 cgtgacaaca ttggtaacga cgccagagga tattctgttg gtatgggcgt tgggacagat
     2161 gctggcagtg gaccgaggaa gaacgccaca gcagtcaacc aacagagtgg tgttatcacc
     2221 gtttataacg ccggaattgg tatggccgcc tacggcgctg gtaatacggt tattaaccag
     2281 ggcaccatta atctggagaa aaacggtaat tacgatagta gcctgggcgt gaattcgctt
     2341 atcggtatgg ctgcatataa cagtggtacc gccattaacg atcaaaccgg tgttatcaat
     2401 attaatgctg acaatgggca ggcattttat agcactggta acggtgtcat tcttaactat
     2461 ggcaccattt gtgttaacac taattgcctg acgggcaatg actataatga tactgactcc
     2521 tatacctcga tattatacac gggtggcgat gtcatcactg cacagagtga aacacaaagt
     2581 ctgaccaaaa aagccacgat tagcgacaaa aaagacggta atgttgttaa cagtggtgcg
     2641 ctttccggtg ctgatattgc gattagtagc ggtaagttag ttaatacatc aacgggtacg
     2701 attaataacg caataattat caacggcggt gcgttaagta acgaaggctc tgtggcgaag
     2761 gtgacgcaaa acgccggtac tttcaataac acagggtcgg ttaacagtcg tatgactcag
     2821 acaggaggaa cgttcaataa ccaacagggc gggacggtga agaacggctc cagactggcc
     2881 aacagcgcta ttgctaataa catggggatc tggtcccttg gcgatacgag tagtggtagt
     2941 aatgccagca tgctggaaat taataataat gccgtgttca aaaacagtgg tgacttcatt
     3001 ctggataata gcaaaaacgc tgttcatctg aatcagtcag gtacgttcta caataccggg
     3061 aaaatgttgc tatccaactc ttcccatagc ggcgtactaa atttctgggg tgggaatggg
     3121 cgctttatta acggcggtat tgcggatgtc acggcgaaat ctttggccgt aagcgccagt
     3181 gatgccggga gcagcaatgc cttcttctgg aaccagagca atggggtcgt caattttgat
     3241 aaagacagcg gcgttgcagt gaaattcatt cacagcaatt acgttgcgca gaatgacggc
     3301 accatgaata ttagcggtaa taacgctatt gcgatggaag gggataaaaa cgcacgactg
     3361 gttaacaacg gcacgatcaa cctcggaacg tcgggcacga ccgacacggg catgatcggg
     3421 atgcaactgg acagcggcac gacagctgat gcggtcattg agaacaatgg cgccatcaac
     3481 atttacgcca acaactcctt tgcatttagc atgttgggtt ccgtaggcca cctcgtgaac
     3541 aacggcacag tgactattgc cgatggagtg acaggttccg ggctaatcaa gcaaggtgac
     3601 agcgttaaca ttgaaggtgt gaacggcaat aacggtaaca atagcgaagt gcattatgct
     3661 gattacactt tgcctgatgt acctggctcg tcggtttctg ttgcggccag caccccgtcc
     3721 agtgacggaa gccagaacaa actgaatggt tatgttgtag gaaccagttc tgacggcagt
     3781 gccggcaagc tgaaagtgaa caatgcaagc ctgaaagggg tttctgttaa caccggattt
     3841 acggctggaa ccagctccac ctccgtgacc ttcgacaacg tcgttcaggg cagtaatctg
     3901 accgacgcag agacgattac atcgacctct gtggtatgga acgcgcaagg cactactgac
     3961 gccagcggca acgttgatgt cacgatgact aaaaatgcct acaccgatgt agcaacagat
     4021 tcatctgtta acaccgtcgc gcaggtactg gatgcgggct ataccaacaa cgagttgtac
     4081 agcagcctga acgtaggcac cacggcggaa ctgaacagtg cgctgaaaca ggtcagcggc
     4141 agccaggcaa caacggtatt caatgaagca cgagtgttaa gtaaccgctt cagcatgttg
     4201 tccgatgcag caccggaagt cgcaaacggt ctggccttca acgtggtagc gaaaggcgac
     4261 ccgcgtgctg aactgggtaa cgacacacag tacgacatga tggcgctgcg taaatctctg
     4321 acgctgactg aacatcagaa cctgagtctg gaatacggga ttgcgcgtct ggagggtaat
     4381 ggttcagata ccgcaggcga taacggagtg acaggcggtt acagccagtt cttcggtctg
     4441 aaacaccaga tggcctttga caacggtatg aactggaata acgccctgcg ttatgatgtg
     4501 caccagctcg acagcagccg ctcggtagcg tacggcgata tcaataaaac agccgatgct
     4561 aatgtgaaac aacagtacct ggagttccgt agtgaagggg cgaaaacctt tgagctgcgt
     4621 gaagggctga acgtcacgcc atatgccggg gttaaactgc gtcacacact ggagggtggc
     4681 tatcaggaac gcaatgcggg tgactttaac ctgagcatga acagcggtag cgaaacggca
     4741 gtggacagca ttgttgggct gaaactggac tatgccggta aggacggctg gagtgcgaac
     4801 gccacgctgg aaggcggccc gaacctgagc tatgtgaaaa gccagcgaac tgcgagcgtt
     4861 tcgggcgcgg gaagccagcg tttcaacatt gatgatggtc agaatggtgg tggtttcaac
     4921 agcctggcga caatgggtgt gaaatacagc agccaggaaa gtgcgttgca ggtagatgca
     4981 ttccactgga aagaagacgg catcagtgat aaaggtgtga tgctaaactt caagaaaacg
     5041 ttctaatttt tagcatgtaa tgcataaacc gcaacgctga tacaggttgc ggttttttta
     5101 ttgccggatg cggtgcttga cgtgtcttaa cctgcctggg gtttcgtgtc tttcagggca
     5161 acagtacaac tcatcccgca atcttgttta caccctcgca ttatttatct cttttcgttt
     5221 ctatactgat ttttcttaat ccgttttatt acagggcagg gtgcgatgag cagcaacaca
     5281 tttactctcg gtacaaaatc cgttaaccgt cttggttatg gcgcgatgca actggctggt
     5341 cctggggttt ttggcccccc aagagatcgc cacgtcgcta taaccgtgct gcgcgaggcg
     5401 ctggcattgg gcgtcaatca tattgatacc agcgactttt atggtccgca cgtcaccaat
     5461 cagattatcc gcgaagcgct ttatccttac tctgacgacc tgacaattgt cactaaaatt
     5521 ggtgcgcggc gtggagagga cgcatcctgg ttacccgcat tttctccggc agagctgcaa
     5581 aaagcggtac acgataatct acgtaatctc ggcctggacg tactggatgt ggttaacctg
     5641 cgcgttatga tgggggatgg tcatggccca gcggaaggat cgattgaggc cagcctgaca
     5701 gtgctggcgg agatgcaaca acaaggtctg gtaaaacatc ttggcctgag taacgtcaca
     5761 ccgacgcagg ttgcagaggc gcgaaagatt gccgaaatcg tctgcgtgca gaacgaatac
     5821 aacattgccc accgtgctga tgacgcaatg attgatgctt tggctcgtga tggcattgcc
     5881 tacgtgccgt tcttcccgct cgggggcttt acgccgctgc aatcctccac gctttccgat
     5941 gttgctacaa gccttaatgc gacaccaatg caggtggcgc tggcgtggct gttacagcgt
     6001 tcaccgaata ttttgctgat tcctggcacc tcttcggtgg ggcatctgcg ggagaatatg
     6061 gcgtccgatg aattagtgct aacggatgac gtgctggcaa cgctggacag tattgcgcaa
     6121 aaggcgggtt aaataaaaaa cctcccactt tcgtgggagg catcataaat cttatgcagc
     6181 aacaatactg tcgatggctg cttttgcgtc agactgtgct ttcgctgcca tttccgggcc
     6241 gtacgcgatc ccttcggcga agacaaattt cacatcggta atgccgataa agcccaggaa
     6301 tgtggacaga tatggcgtta ccagatccgt tgggccatct ttatggatcc caccgcggct
     6361 ggtgataacg atggcttttt taccggtgac caggccttcc ggacccttct cggtatagcg
     6421 gaaagtaacg cctgcacgtg caaccaggtc aaaatagttt ttcaactggg tcgagatatt
     6481 gaagttatac atcggtgccg caataacgat aacgtcgtgg gctttcagct cggtaatcag
     6541 ctcatcggaa agcgccagag cttcctgctg acgcggagtc agcggcgcat cgctcggacg
     6601 cagtgcacca accagttcac catccaggac cggaatcgga tttgcagcca ggtcacgcac
     6661 ggtgatttca tcagcggagt gcttttcgcg ccattgttca acaaaataat cggacaactg
     6721 attagactga gagtaccctg ccaggatgct ggatttaaga actaatacct tgctcatggt
     6781 gtttccttat agatatttga atgggcaatg ccccgttgct tgttgacact ttattcacaa
     6841 tcctgccaca gagatagcgc aataaatcga agcctatgtt cgaatttatt gaacaacgca
     6901 tagaaagctg cgatgtggta ctctatatct atcatttaaa agaaaattaa tcaggcagcc
     6961 tactgcccac taacgttatg acagaacaac aaaaattgac ctttactgcc ttgcagcagc
     7021 ggctggattc gttgatgctg cgtgacagat tgcgtttttc tcgccgtctg cacggtgtga
     7081 agaaggttaa aaatcctgat gcacaacagg ccattttcca ggagatggcg aaagagattg
     7141 accatgcggc agggaaagtc ctgctgcgtg aagcggcacg accggagatt acttacccgg
     7201 acaatttgcc ggttagtcag aaaaagcagg acattctcga agcgattcgt gaccaccagg
     7261 tggtgatcgt cgccggggaa acgggttccg gtaaaacgac tcagctaccg aaaatctgta
     7321 tggagctggg gcgcgggatt aaagggctga tcggccatac ccagccgcgt cggctggcgg
     7381 cgcgaacggt ggcgaaccgt attgcggaag agctaaaaac ggagccgggc ggttgcatcg
     7441 gttacaaagt gcgtttcagc gatcacgtca gtgataacac gatggtcaag ctgatgaccg
     7501 acggtatcct gctggcggag atccagcaag accgcctgct gatgcagtac gacaccatca
     7561 ttattgacga agcgcacgaa cgcagcctga atatcgattt tctgctcggc tatttgaaag
     7621 agttgctgcc gcggcgtcct gacctaaaaa tcattatcac ttccgcgact atcgacccgg
     7681 aacgcttttc gcgccacttt aataatgcgc cgattatcga agtctccggt cggacctatc
     7741 cggtggaagt gcgctatcgc ccgattgttg aagaagccga tgataccgag cgcgatcagt
     7801 tgcaggcgat ttttgacgcc gtagacgaac tgagccagga aagccctggc gacattctga
     7861 tctttatgag cggcgagcga gaaattcgcg ataccgccga tgcgctgaac aagctgaact
     7921 tacgccatac cgaaatcttg ccgctttatg cacggctttc caacagcgaa cagaaccgcg
     7981 tgttccagtc gcacagcggg cgacgcattg tactggcgac caacgtcgcg gaaacctcgc
     8041 tgaccgtacc gggtattaaa tacgttatcg acccaggtac agcgcgtatc agccgctaca
     8101 gctatcgcac caaagtgcag cgtttgccga ttgagccaat ttcgcaggct tcagctaatc
     8161 agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg tattcgtctc tattccgaag
     8221 acgatttcct ttcgcgcccg gagtttaccg atccggagat tctgcgtacc aacctggcct
     8281 cggttatttt gcagatgacc gcactgggct tgggcgatat cgccgcattc ccgtttgtcg
     8341 aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga
     8401 tcaccactga tgaacagcaa agtgcttata aactgacgcc gctcggtcgt cagctctcgc
     8461 agttgcctgt cgacccacgt ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg
     8521 tgcgtgaggc gatgattatc acgtccgcgc tctccattca ggatccgcgc gagcgtccga
     8581 tggataaaca gcaggcagct gacgaaaaac atcgtcgctt ccacgacaaa gagtctgact
     8641 tcctggcatt tgtgaatctg tggaattatc ttggcgagca gcaaaaggcg ctttcttcaa
     8701 acgccttccg tcgcctgtgt cgtaccgatt atctcaacta tctgcgcgtg cgcgaatggc
     8761 aggatatcta cacccagttg cgccaggtgg tgaaagaact tggcattccg gttaacagcg
     8821 aaccggcgga ttatcgcgaa attcacattg cgttgctgac cggtttgctt tcgcatatcg
     8881 gcatgaaaga tgccgataaa caagaatata ccggcgcacg taacgcgcgt ttctccatct
     8941 tccccggttc tggtttattc aaaaagccgc cgaaatgggt aatggtggcg gaactggtag
     9001 aaaccagccg cctgtggggg cgcattgctg cgcggatcga cccggaatgg gtggagccag
     9061 ttgctcagca tttgattaaa cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg
     9121 cggtgatggc aacggaaaaa gtcactgttt atggtttgcc gattgttgcc gcgcgcaagg
     9181 tcaactacag ccagatcgat ccggcgttat gtcgtgaact ctttattcgc cacgcgctgg
     9241 tggaaggtga ctggcagacg cgtcacgcat tcttccgcga aaacctgaaa ctacgggcgg
     9301 aagtagaaga gctggaacac aaaactcgtc gccgcgatat tctggtggat gacgaaacgt
     9361 tgtttgagtt ctacgaccag cgcatcagtc acgatgtgat ctctgcacgc catttcgaca
     9421 gttggtggaa aaaagtcagc cgcgaaacgc ctgatttgct caactttgaa aagagcatgt
     9481 tgatcaaaga aggggcggaa aaaatcagca agctggatta cccgaacttc tggcatcagg
     9541 gcaatctcaa gctgcgtttg agttatcagt ttgagccggg ggcagatgct gacggcgtga
     9601 ccgtacatat tccgctgccg ctgcttaacc aggttgagga gagcgggttt gagtggcaga
     9661 tccccggcct gcgccgtgag ctgatcatcg ccctgattaa atcgttgccg aagccggtac
     9721 gccgtaattt tgtacctgcg ccaaactatg ccgaagcgtt tttaggccgc gtcacaccgc
     9781 tggaattgcc gttgctcgac agccttgaac gagagcttcg gcggatgact ggcgtcaccg
     9841 ttgaccgcga agactggcac tgggatcagg tgcccgatca cctgaaaatc accttccgcg
     9901 tggtggatga caaaaacaag aagctaaaag aagggcgctc attacaggat ctgaaagacg
     9961 ccctgaaagg caaagtgcag gaaacgctgt ctgccgtggc ggatgacggt atcgagcaga
    10021 gcggtttaca tatctggagt tttggtcaac tgccggaaag ctacgagcag aagcgcggca
    10081 actacaaagt gaaggcttgg ccggcgctgg tggatgagcg tgacagcgta gcgatcaaac
    10141 tgtttgataa cccgctggaa cagaagcagg caatgtggaa cggtcttcgc cgtttactgc
    10201 tgctaaatat tccatcgccg atcaagtact tgcacgaaaa gctgccaaac aaagccaagc
    10261 tggggctgta ctttaacccg tatggcaaag tgctggagct gatcgatgac tgtatctcct
    10321 gcggtgtgga tcaactgatc gacgccaatg gtggcccggt ctggacggaa gaaggctttg
    10381 ctgcgctgca tgaaaaagtc cgtgctgaac tgaacgacac ggtggtggat attgcgaagc
    10441 aggtcgagca aatcctcact gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg
    10501 atatgaccat ggcgctgggg ctttctgaca ttaaagcgca gatgggcggg ctggtgtatc
    10561 gcggttttgt cactggtaac ggcttcaaac gactgggcga cacgctgcga tatttgcagg
    10621 caattgaaaa acgactggaa aaactggcgg tcgatccaca tcgcgaccgt gctcagatgc
    10681 tgaaagtcga aaacgtccag caggcgtggc agcaatggat caacaaacta ccgcctgcac
    10741 gtcgtgagga tgaagacgtg aaagaggtcc gctggatgat agaagagctg cgcgttagtt
    10801 acttcgctca acaacttggc acgccttatc cgatttcgga taagcgtatt ttgcaggcga
    10861 tggagcagat tagcggttaa cactgctatt tgcctgataa atctaaaacc cggtaagcat
    10921 ttagcgccgg gttttttatt aattctaaaa acgataaggg aaaaaattca ggagttcaga
    10981 aaaatacaat tctctgctgc aagatgaata atgtttatct acagcatttc cttaaaagat
    11041 atgtcaggct tgcggagtgg cagttcagga aatacgactt cctcctttca gagtgctccg
    11101 cttctcacta ttatctcacg cagtattctt aagggaacga taaggaggaa ccatgaatat
    11161 taccccgttt ccgacgcttt cgccggcaac catagatgcc ataaatgtta tcggacagtg
    11221 gctggcgcag gatgatttct ccggtgaggt gccgtatcag gccgattgcg tgatccttgc
    11281 aggcaatgcg gttatgccga ctatcgatgc ggcatgtaag attgcccgcg atcagcaaat
    11341 tcctttactg attagtggtg gtatcggtca ctcgacaact tttttgtata gcgccatcgc
    11401 acagcatccg cactacaaca ctatccgcac cactggcaga gcagaagcta ccattctggc
    11461 ggatatcgct catcagttct ggcatattcc gcatgaaaaa atctggattg aagaccagtc
    11521 aacaaactgc ggtgaaaacg cacgctttag catcgcgtta ttgaatcagg ctgtagaacg
    11581 agttcatacg gctatcgttg ttcaggaccc caccatgcag cggcgcacga tggcgacgtt
    11641 ccgccgtata accggggata atcccgatgc accacgctgg ttaagttatc ccggattcgt
    11701 tcctcagtta ggaaataacg cagagagtgt aatctttgtt aatccgttgc aaggattatg
    11761 gccggttgag cgttatctat cgctactcac tggcgagctg ccgcgtttac gcgatgatag
    11821 cgatggctac ggcccccgcg ggcgagattt tatcgttcac gttgattttc cggcagaagt
    11881 cattcaggca tggcaaacgc tgaaacatga tgcggtgctc atcgaggcga tggaaagtcg
    11941 ctcgttacgt taaaaattgc ccgtttgtga accacttgtt tgcaaacggg catgatttct
    12001 gacttttatt tctgcctttt atttctgcct ttaattcctt ttacacttgt ttttatgaag
    12061 cccttcacag aattgtcctt tcacgatgcc gtctctctga tgattgatgt taattaacaa
    12121 taaatttacc aaaaacaaac atgcaaatca caggagtcgc ccatgtcagt acccgttcaa
    12181 catcctatgt atatcgatgg acagtttgtt acctggcgtg gagacgcatg gattgatgtg
    12241 gttaaccctg ctacagaggc tgtcatttcc cgcattcccg atggtcaggc cgaggatgcc
    12301 cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat gggaagcgtt gcctgctatt
    12361 gaacgcgcca gttggttgcg caaaatctcc gccgggatcc gggaacgtgc cagtgaaatc
    12421 agtgcgctga ttgtcgaaga agggggcaag atccagcagt tggctgaagt cgaagtggct
    12481 tttactgcgg actatatcga ttatatggcg gagtgggcac ggcgttacga gggcgagatt
    12541 attcaaagcg atcgtccagg agaaaatatc cttctgttta aacgtgcgct tggcgtgact
    12601 accggcattc tgccgtggaa ctttccgttc ttcctcattg cccgcaaaat ggctcccgct
    12661 cttttgaccg gtaataccat cgtcattaaa cccagtgaat ttacgccaaa caatgcgatt
    12721 gcattcgcca aaatcgttga tgaaataggc cttccgcgcg gcgtgtttaa ccttgtactc
    12781 gggcgtggtg aaaacgttgg gcaagaactg gcgggtaacc caaaggttgc aatggtcagt
    12841 atgacaggca gcgtctctgc aggtgaaaag attatggcga ctgcggcgaa aaacatcacc
    12901 aaagtgtgcc tggaattggg gggtaaagca ccggctatcg taatggacga tgccgatatt
    12961 gaactggcag tcaaagccat cgttgattca cgcgtcatta atagtgggca agtgtgtaac
    13021 tgtgcagaac gtgtttatgt acagaaaagc atttatgatc agttcgtcaa tcggctgggt
    13081 gaagcgatgc aggcggttca atttggtaac cctgctgaac gcaacgacat tgcgatggga
    13141 ccgttgatta acgccgcggc gctggaaagg gttgagcaaa aagtggcgcg cgcagtagaa
    13201 gaaggggcga gagtggcgtt gggtggcaaa gcggtagagg ggaaaggata ttattatccg
    13261 ccgacattgc tgctggatgt tcgccaggaa atgtcgatta tgcatgagga aacctttggt
    13321 ccggttctgc cggtggtcgc atttgacacg ctggaagagg ctatctcaat ggctaatgac
    13381 agtgattacg gcctgacctc atcaatctat acccaaaatc tgaacgtcgc gatgaaagcc
    13441 attaaagggc tgaagtttgg tgaaacttac atcaaccgtg aaaacttcga agctatgcaa
    13501 ggcttccacg ctggatggcg taaatccggt attggcggcg cagatggtaa acatggcttg
    13561 catgaatatc tgcagaccca ggtggtttat ttacagtcgt agtgagtgaa agaggcggag
    13621 gttttttcct ccgcctgtgc gcgtcagagt ttagcgaatt tttcgagggt gcgaataagc
    13681 tgcgtgacga agccatattc gttatcgtac caggcgaccg ttttcaccag ttgtaaatcg
    13741 cccacggcgg taatttccgt ttgcgtggca tcaaacaccg aaccgaaatg gctgccaatg
    13801 atatcggaag agactatttc ttcatcggta taaccaaatg actcgttatt gtttgtcgcc
    13861 tttttaagcg cgttgttcac ctcttcggca gtcacttttt ttccgagaat cgacaccagt
    13921 tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag tttgccgctc
    13981 agttccggga tcaccaggcc aatggctttt gccgcccccg tagtgtgggg aatgatattt
    14041 tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac cagtgactgg
    14101 gtgccggtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa actgtcatgc
    14161 aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg acgccacgga aacaatggtg
    14221 tcgttgccat ccagagtgtc gtcattgacg ttataaacga tggttttcat ttcaccggca
    14281 ggggcggaaa tcaacacctt cttcgcgcca gcatcaagat gcgcctgcga tttctcggcg
    14341 gaggtataaa agcctgtaca ttcgacaatg atttctgcgc ctttcgcttt ccacggaata
    14401 tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat aagtgaatct
    14461 tccgtataat caacgctcca ggggaacggt ccgtagtttg aatcatgttt cagcaggtag
    14521 gcgagaattt ttggggaagt gagatcatta atagcgacaa cgtctatgtt gcttttgact
    14581 tcaagtaatc gacgcaacac cagtcgaccg atacgaccaa aaccattaat accaacttta
    14641 ctcatggtct tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc aggcttagac
    14701 caggatggcg ggatgggcaa tctacattct tgcagtgaaa cgtaacgtac tgaaaacggg
    14761 tgaacaatat ttaatgaaat tttaagaaaa gcccgttatg ttaacggcaa attatgttaa
    14821 agcaggaaat gttatgggaa ataaatactc aaggttacaa atcagcattc actggctggt
    14881 ctttttgctg gttatcgtag cgtattgcgc aatggagttt cgtggtttct tcccacgtag
    14941 cgatcggccc ctcatcaaca tgattcatgt ttcctgtggc atctcaattc tcgtactgat
    15001 ggtggttcgt cttttgttaa ggctgaaata cccaaccccg ccgattatac ctaagccaaa
    15061 accgatgatg acgggactgg cgcatttggg ccatttggtg atttatctcc tgtttattgc
    15121 gctgccggtg attggtttgg tgatgatgta taaccggggc aacccgtggt ttgcgtttgg
    15181 tttgacgatg ccttacgctt cagaggccaa tttcgagcgg gttgatagct taaagtcgtg
    15241 gcatgaaacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg cggctgcact
    15301 ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccac gtaaacgttc
    15361 ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat
    15421 acgggcctac atcggaagcg cctattatat ttatttgtat gacaaataaa accataatcc
    15481 ttgtctatac gtccatctgg cttatcttta atcaattcac ccgatctttg atctcatcaa
    15541 cggtatcaaa ataaaccagc gtataacgtt caaatcgaac ataagattcg ataaatcatg
    15601 gaggttatat gaaaaaactg gcacttatcc tggttatggg aacgcttgtt tccttttatg
    15661 ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac tgtacggcag
    15721 gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca tgcactaact
    15781 gaagtgtaaa aggggggcca tgagaaaatg aatccgctag tcagatgcgc ggggaaaacc
    15841 cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt
    15901 cccggaaccg cagttcgtag agcgtttgtc gggtcaggag tgtgaatgtc agaatcgtaa
    15961 tgcagataat gagcagacac accaccagag ggttgtgctt catagccttt ctccttgccg
    16021 gatggcgggt aagaggctaa gatctgaatt gctaggttca ttcgttggcc tcggttgata
    16081 gaaatatcgg tcggggcctt cgtctttctg attccaggtt agcctgaaaa cagaaagtct
    16141 caggcacccg tgagcatcct atgtggtttc cttagggacg aaaataatca cctgacgaaa
    16201 tcgcgtgctg ctttccagaa tttttcgtca ttcggtttag ccagtttaat cattcgttac
    16261 tctcgcaagg ccgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagccgca
    16321 gcgcccaggc ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc
    16381 tgggatcgga tgaggtggca acggcaggga aaaccatgtg cactattgtt gatgccgtcg
    16441 cgagtgtcac acatatcatg caggaaatcg ccaccgcctc gggtgaacaa agtcgaggca
    16501 taacgcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc
    16561 tggtagaaga ggcctcaacg gcggcggtgt cccttgaaga acaggcggca cgactaactg
    16621 aggcggtgga cgtattccgt ctgaacaaac attctgtgtc ggcagaacca cgaggagcgg
    16681 gtgaaccagt tagttttgct acggtgtgaa aatgttaaag gagggatcga cagatccctt
    16741 cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga
    16801 gaacgtcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac
    16861 aggcagggct accagcgtgg catgacgcag gtcgtctttt acagcgccag aagggacaaa
    16921 ccacacgtaa tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat
    16981 acaacccgaa ggaattttac atccctggct ctgcactaat gcatctgaat gctggcgtgg
    17041 cgcagtgcct tctggtgata caacgaccgg ccattccagc acccggctta gcgttacgtt
    17101 ctcctgaagt agcgggtgat tagggcggac aaccagcttc agcgattcaa gaaacagtag
    17161 ttcgtaatta agcccgttca tcagttcagg atctgacatc cgaccaatgc cgatatcgat
    17221 ttccccggtt tttaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt
    17281 cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ctagtgccgc
    17341 agtaggtagc gcaccaaccc tgacgacatc attattaaga ccttctttac gatgaagcga
    17401 ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc
    17461 gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc ccgtcagctg
    17521 ctccagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc
    17581 gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat
    17641 gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct
    17701 gacaagtgaa gttgttttat tatgataact tgattgcaaa atattattaa caattaaagc
    17761 aactatgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa
    17821 attcttccag ccggagtgaa tgcggttacc tacactccag attactgacc actggaggca
    17881 gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg
    17941 tcagccatgt accagcaaga agttccgcag tacggcacgc tgctggaact ggtagctgat
    18001 gtaaatctgg cggtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagac
    18061 gaactggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag
    18121 cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat
    18181 ctctcgcagg caggggtgcc ggtacattcg acagcatttc ggcccattga tgctgcttct
    18241 ctggcgcgta atcccttccg cgtttttacc tcattactcc gccttgagct tatcgagaac
    18301 gaattattac gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt
    18361 tgtcgacaac tgttacagga atatgagcag cggggcggtt ttaacgaaac acaggcacag
    18421 gagtttgtgc aggaagccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa
    18481 aaaacctatc gcgccttgca caaagaacat cggttgattg ctgatgtggt ctgttttcct
    18541 ggatgccata tcaaccacct gacgccacgc acgctggata ttgaccgggt gcagtcgatg
    18601 atgcctgaat gcggaattga acccaaaatt ctgatcgaag ggccgccgcg ccgcgaggtg
    18661 ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg
    18721 cagaaacagg gcacacatac cgcgcgcttt ggtgaaattg agcagcgtgg tgtggcatta
    18781 acgccgaaag ggcgacaact gtatgatgat cttctgcgga acgctggaac cgggcaggat
    18841 aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt
    18901 ttaatgcgtc agcaagggct gggatggttc cggtaccgtc tgacgccttc gggtgaggcg
    18961 catcgtcagg cgattcatcc cggagacgat ccacagccct taatagaacg tggttgggta
    19021 gtggcgcaac ccattaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag
    19081 tcaaatctgg gtaatgaaac gcaggcatgc aatcacggtg atgccagtcg cgaagcattt
    19141 gagcaggcgt tggggtgtcc ggttttggat gagttccagc tttatcagga agcggaagaa
    19201 cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg
    19261 cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc caatttatgc
    19321 atattctctg atttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata
    19381 aataagcact ttcttgccgc tgaaaatgac cagcgcggga ccattcacaa caccagaagg
    19441 actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg
    19501 tgtgcggtac cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gccgattctg
    19561 atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg
    19621 acttagcgca aacagcatgg cgtggtgcac cacgtccgtt acctgatact ctggcgacaa
    19681 tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg
    19741 ttgagaaccg tcaactggac gctcagtttt tccatatggg aatgggattc cgtcgccgcg
    19801 ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg
    19861 agttgttcaa atacaacgat gcgggtgtgg ataccaaaca attagaaggg caaagcgatc
    19921 tcggttttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat
    19981 cattcctcgg cgcgagttat ttccgcgccg ttgatgacac atatcaatac ggtttgtcgg
    20041 cccgcggcct ggcgatcgac acttacaccg acagcaaaga agagttcccc gactttaccg
    20101 ccttctggtt tgatacggta aaaccagggg caaccacctt taccgtttat gcgttgctcg
    20161 atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa aatcaggtga
    20221 ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcac
    20281 cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgac accattcatc
    20341 cgcaaatcca tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc
    20401 gtccgctgaa caatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag
    20461 ggtttggttt attgcaactg gatcgtgatt tctcccatta tcaggacatt atgggctggt
    20521 ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag ggcaccatcg
    20581 gcctgatgga aatcccaact acgggggaaa cgctggataa cattgtttgc ttctggcagc
    20641 cagaaaaagc tgtaaaagcg ggtgatgagt ttgcattcca gtatcgtctg tactggagtg
    20701 cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg
    20761 gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg cgacgttttg
    20821 ccgtcgattt cgttggtggc gatctgaaag ctgccgcgcc aaaaggcatt gagccggtga
    20881 ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccattgatg
    20941 gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc
    21001 ggatgtatct gcgttgtcag ggcgacgcta tcagtgaaac atggttatat cagtatttcc
    21061 cgccagcgcc ggataaacgt cagtatgttg atgaccgcgt gatgagttaa tcgttttttc
    21121 ttcggcatct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg cattttttcc
    21181 taagttaaat gagtaatctg ctggtgtgta tttcagatac accttgtcag ccactaacag
    21241 ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct
    21301 cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa
    21361 ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag
    21421 ttaaaagatg agatgctcaa aatcctgcag caggtgagcg tcaaagaggt gtaaactttc
    21481 ctaagccgcc agccaggcgg cttttttaac agctgcatgg attgactgga gataagatga
    21541 ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcatgtca
    21601 cacttcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc
    21661 cgcagtttgt tcaaagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc
    21721 atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta
    21781 tctcttttaa tcgtattgaa ccactgaata aaaccgctga aatcggctac tggctggacg
    21841 aacctcatca ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg
    21901 ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca
    21961 accaggtagc gttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagtttc
    22021 tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca caataaccct
    22081 gccagcagcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga
    22141 atcgtgtcgc catcaaaggc ttcaacgacg gcgtggtcag ttaactccac atggttttca
    22201 attatcaccg cacctgtaat acggctctca ccttgtatta caacgtgctc gtcgagcaga
    22261 atcggtccgc cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa
    22321 ttaccttcca caatggcata ttccgccacc tgtgaactgt aatgaatcgt cggaatggca
    22381 tcttcttcta tgccagcttt cacctgcgcg tgaccgtaaa ctttagcgca atcgcatagc
    22441 cagacgttgt tctcttcatt accttcaata ctggcaaaat caaaaacttc ggcgcgatgt
    22501 tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc
    22561 gaggcgctaa cctttgcgcg atcataaatt tgtagcagga gttgatggtc gggcgttaag
    22621 ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat
    22681 tgaccgcaaa ctaatgagtc actaatggtg acactgtcac ttatatatgc accctggctg
    22741 atttcactat tatcgatcca gacattatcc gttgcgtaaa cctctcccca cagcacgctg
    22801 gtgccggtga tacgcgtatt cccggaaatc accgttccgc cgaacgcaat agcattctgg
    22861 tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat ccagccgcca
    22921 gcgcttcctg ctatcacatc gttaaaatcg ctcatggcga taatttgccg taataacaca
    22981 ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat
    23041 ttacgcatag cgttttccca caggtgactt actataaccg tagcaaattc tgcggctctg
    23101 gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa
    23161 tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg
    23221 tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca
    23281 gccactggtt gggggatggg ctggtgattc tggcgatgat catctgggga ttattgactg
    23341 gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcagaa gtgcgccatc
    23401 cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt
    23461 ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt
    23521 tggcttatgc cgcctggcaa actgcgggat tatggcgtgg atctcaccct gaagaagcta
    23581 ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg
    23641 gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc
    23701 taagcctgga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac
    23761 tgaggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt gcctggctga
    23821 gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat ggactgctgc
    23881 aactgctgtt tatgctatgt ctgatgccat ggtatctctc ccagccattt aatgcttcat
    23941 tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg
    24001 gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt accaatttta
    24061 ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca
    24121 gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc gtgatgaaaa
    24181 ctattttact gataaatatg aattaacccg cacacactct gaagtgctgg aagcggtgaa
    24241 agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta
    24301 cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa
    24361 cgtcgagcgc attaagtcca ttgaaaatct ggataattta cacacccggg tcgttgatct
    24421 gaataacctc acatttgatg gacagtacga ttttattctt tcgactgtgg tgctgatgtt
    24481 ccttgaggct aaaaccatcc cagggctgat tgccaatatg caacgttgcg ctaaacctgg
    24541 cggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt
    24601 cccgtttgcc ttcaaagagg gggaattacg tcgatattac gaaggctggg agatggtgaa
    24661 atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc gtattaaact
    24721 gcgtttcgcc acgatgctgg cacgtaaaaa atgatccggt aagaacaaag cgcgtgaaaa
    24781 ttccccacgc tgagatggtt tactgttctt cttttcggta agcatatttt ttatcgaagg
    24841 gatgtgaaat taatcacagt agtcgaagtt ttttagccgc ttaacgtact gaaatttaac
    24901 tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctaa
    24961 aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa
    25021 cttaaaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa
    25081 gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat cacccagcca
    25141 gataaatatt ctggtttttt aaacaattac tctgatttaa aagaaacaac ctcggcaaca
    25201 ggtaaacctg ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc
    25261 gtctggaacc caatcactta ttatccggta ccgaaacctt ctacccaggt agggcagaaa
    25321 gttctggata aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt
    25381 aaaccactgg ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt
    25441 gtagatacca gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt
    25501 gcggggacgc aaatggctac aggccaccgt accatggata ctcgcttgta ttttgaaggt
    25561 gagctgattg atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt
    25621 aaagacctga ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat
    25681 gacatggcga ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat
    25741 cgttagatgg cgctttttat tatcggtttc tgttagcgat taaattcaac accacgtaac
    25801 aaacgcctcc ggcaattaat ccccaaaacg cagaaccaat cccgaacagc gtcaatccac
    25861 ttgccgttac cagaaatgcc accaccgccg cgtcgcgctc acgctcatta tgcagcgcct
    25921 gatacaaact gccgccaatg gtactcaaca gcgcaagacc tgccagcatc tggatccaac
    25981 ttacgggcag ggcagccatc atcccggtaa tggcactacc aaacagacct gcgatcaaat
    26041 aaaaaatgcc ggcaacggca gcggccagcc aacgttgatc tttatccgga tgcgcttccg
    26101 ggctttggca aatagccgcg gtgattgccg caataccgac ggaataaacg ccgaaagggg
    26161 aaaaaaacag tgccagcaat ccagtaaaca caatcaacgg cgaaacagga gccgaatacc
    26221 cggctgcttt cattgctgcg atacccggtg cgttttgcga tgccatcgtc accagaaaaa
    26281 gggggagtgc aacgctcaga ctgtgagcaa acgaaaaatc aggggtaata taagtgggga
    26341 gaacgggttt aaagacaata tcagttgtaa caacgtcacc ttgcgcggta acgatgacga
    26401 tcccaataat catcgcggca attaccgcat agcgcggcgc aacggccttg gctgccagcc
    26461 atgccagcaa catactgcca cacaacgtaa attgaccatc cagactggca aacgcctgta
    26521 aaccaaaacg taataaaatc ccggcaagca ttgccgccgc aagcgagtgc ggaataatgc
    26581 gcatcagacg agcaaagagt cccgttatgc cgcagaggac tattaacgcg ttggtgacaa
    26641 taaaaacgcc gatggcttcg ttaagtgtta gttcctgcaa tccggtgacc aacaaagccg
    26701 cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata ccataatgtc agagtcagcg
    26761 tactgacgcc cattgccagc cccagcgccg tcatccagcc agagatttgt gcagtggtgg
    26821 ctccggcgac aatcgctgct tgccagatta ttgccgctga actggcgtag ccaattaata
    26881 ccgccagaaa ccccgccagt agcgtgggtg gaggaataga aaacagacgc atagtcacct
    26941 cgtgcgttat aacgtacagg taccgtagca tttgtccgtt ataacgcaca agtgataaac
    27001 ttccgttttg ccggaggagt cgcatggaaa atctcgctcg ttttttatcc accacactta
    27061 aacaactacg tcagcagcgc ggctggagtc tttcgcgact ggcagaagcg acgggcgttt
    27121 ctaaagcgat gttagggcag attgagcgta atgaatccag cccgacggtc gcgacattat
    27181 ggaaaattgc caccgggttg aacgtgccat tttcaacatt tatttctccg ccgcagtccg
    27241 ctacgccttc agtttatgat ccacaacggc aggcgatggt gatcacttcg ctgtttcctt
    27301 atgatccaca actctgcttt gaacacttct cgattcagat ggcacccggt gcaataagcg
    27361 aatcgatgcc ccatgaaaag ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg
    27421 atctgtgtgt tgatggtgaa tggcagagcc ttaattgtgg ggaaggcgtt cgatttgctg
    27481 cagacgtcac gcatgtctat cgtaatggtg gggagcaaac cgtacatttt cattccctca
    27541 tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa
    27601 tagccgccat ttttcagcta ctggataaga aagtgaccgt atcttctcat cgtcttgaac
    27661 tgttaagccc ggcacgcgat gccgccattg cccgcgaagc tattttgcac ggtgccgatg
    27721 ctgtttatat cggcggccct ggttttggtg cccgtcataa tgccagtaat agcctgaaag
    27781 atattgccga gttggtgccg tttgcccatc gttatggtgc aaaaattttc gtcacgctta
    27841 acaccatttt gcatgatgat gagctggaac ccgcgcaacg gctgattact gacctctacc
    27901 agaccggtgt cgatgcgctg attgttcagg atatggggat tctggaactt gatattccgc
    27961 cgattgaact gcacgccagt acgcagtgcg atattcgtac agtagaaaaa gcgaagttcc
    28021 tctctgatgt tggcttcacg cagattgtgc tggcgcgaga actgaatctt gagcagatcc
    28081 gcgcgattca ccaggccacg aacgcgacca ttgaattctt tattcatggc gcattgtgcg
    28141 tggcctattc gggtcagtgc tacatttctc atgcgcaaac agggcgtagc gccaaccgtg
    28201 gcgattgctc gcaggcgtgc cgtttgccat acacattgaa agacgatcag gggcgggtgg
    28261 tttcctatga aaaacatctg ctgtcgatga aagataacga tcagactgcc aacctcggcg
    28321 cgctgattga tgctggcgta cgctccttca agattgaagg gcgttacaaa gatatgagct
    28381 acgtgaagaa tatcaccgcc cattatcgcc agatgctgga tgccattatt gaagaacgtg
    28441 gcgatctggc gcgcgcttca tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa
    28501 agactttcca ccgtggtagc acagattatt ttgtgaatgc ccgtaaaggc gatattggcg
    28561 cgttcgattc gccgaaattt atcggcctgc cggtgggcga agtattgaaa gtagcgaaag
    28621 atcatattga tgttgccgtt accgagccac tggcaaatgg cgatggcctg aacgtgatga
    28681 ttaaacgtga agtcgtcggt tttcgtgcca atacggtaga gaaaaccgga gaaaatcagt
    28741 accgcgtctg gccaaatgaa atgccagcag atttgcacaa aattcgcccg catcacccac
    28801 taaaccgtaa tcttgatcat aactggcacc aggcactgac aaaaacctcc agcgaacgtc
    28861 gggtggcggt agacattgaa ctgggcggct ggcaggaaca actgattctg accctgacca
    28921 gtgaagaggg cgtcagcgtc acgcatacgc tggacgggca gttcgacgaa gccaataacg
    28981 ccgaaaaagc aatgaacaat ctgaaggatg gtctgacaaa actggggcaa accatctatt
    29041 acgcccgcaa tgtgcaaatt aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa
    29101 accagttccg ccgtgaagcc gccgatatgc ttgatgctgc gcgtcttgcc agttaccagc
    29161 gcggcagccg taaaccggtt gctgatcctg cgccggttta tccgcaaacg catctgagtt
    29221 tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta tcatcgctat ggtgtgcaac
    29281 tgattgacgc ggcgtatgag gcacatgaag agaagggcga agtcccggtg atgatcacca
    29341 agcattgtct gcgctttgcc ttcaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa
    29401 gctggaaggc gacgccaatg caactggtta acggcgatga agtattaacg ctaaagtttg
    29461 attgccgccc atgcgagatg cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc
    29521 cgttaccggg aagcgtagtg gcatccgtaa gtccggatga tctgctgaaa acattgccga
    29581 agcgaaaagg gtaaaacgcc agttttcggg ttactcacca ctcatcaaat ctgcatgata
    29641 ttgcctgccg ggtaaggcgt catgccgcat ccggcaacaa atgactcaac gctgtcccgc
    29701 ttctggtttg cgcgattttt gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc
    29761 cgccacagca cgcaattcat cgctgtccgc ttcatgacgc agctgatgat ccacattctt
    29821 tacccacata aattcatgtc ctttgtgccc tgccatgagt tgtcctgaga acagtgcaca
    29881 cgttaataag acaaccgata acgccttcga aaacatcccg ccaccttttt ctaacctttt
    29941 gccgctatga tgccgatcgt ttcttgaggt tattattcag ttttgcaaat tagagcaaag
    30001 aaattctgga atcttccttc ctgattttgc attgaattct gccgttgcgg cgatttagtg
    30061 ctattttcga gcacattaca cacggaggta aacttcgcca ttaaatagtc acgatcactt
    30121 tattgaagtg cctttgagcg tcgcctctaa tgtattgctg ttaaatgctt ttttacagtc
    30181 agaaatcact cagcaagagt tagccaggcg aattggcaaa cataaacagg agattactcg
    30241 cctatttaat ttgcaccatg cgacaaaaat cgacgccgtc cagctcgcgg ctaaggcgct
    30301 tggcaaagag ttatcgctgg tgatggttta attacagtta acgaaaagtt gtcattttta
    30361 acaactgata tagactgccg aatcatctgc acataattac gattcgataa tgaaaaaata
    30421 ccagcagctt gcagaacaat tacgcgagca gattgcgtcg ggtatctggc aacccggcga
    30481 tcgtttgcct tcgttgcgtg accaggtggc gctttctggc atgagcttta tgactgtcag
    30541 ccatgcctat cagttgctcg aaagtcaggg atatattatc gcgcgaccgc aatcgggtta
    30601 ttacgttgcg ccacaggcaa taaaaatgcc gaaagcgaca gtcattccag tcacacgaga
    30661 tgaagcagtc gatatcaaca cctatatttt tgatatgttg caggccagtc gcgatccgtc
    30721 ggtagttccg tttgcctcgg cctttcccga cccgcgactt ttccccctcc aacaactaaa
    30781 ccgctcgctg gcgcaggtaa gcaaaaccgc cacggcgatg agcgtgattg aaaacttacc
    30841 gccaggaaac gcagaactgc gtcaggctat tgctcgtcgc tatgccttac agggcatcac
    30901 catttctcct gatgaaattg tcattaccgc cggggcgtta gaggcattaa acctcagttt
    30961 gcaagcggta actgaaccgg gcgattgggt gatagtagag aatccttgtt tctacggcgc
    31021 gttgcaggcg ctggagcggt tacggctgaa ggcgttatcg gtagcgacgg atattaaaga
    31081 agggatcgat cttcaggcgc tggaactggc gttgcaggag tatccggtga aagcgtgctg
    31141 gctgatgact aatagccaga acccacttgg atttacctta acgccgcaga aaaaagtgcg
    31201 gctggtagcg ttgctcaatc agtacaacgt aacgctgatt gaagatgacg tttatagcga
    31261 actttatttt ggacgggaaa aaccgctgcc tgcgaaagcg tgggatcgcc acgatggtgt
    31321 tttgcattgc tcttcgtttt cgaaatgtct ggtgcctggt tttcgtattg gttgggtcgc
    31381 cgccggaaaa catgcacgta aaattcaacg cttgcagttg atgagtacgc tttccaccag
    31441 ctcaccgatg cagcttgcgc tggtggatta cctttctact cgccgatacg atgctcatct
    31501 tcgtcgcctg cgtcgccagc ttgcggaacg taaacaacgc gcctggcagg ctttgctgcg
    31561 ttatctgcct gcggaagtca aaattcatca caatgacagc ggttattttc tctggctgga
    31621 gctccccgag ccgttagatg caggagaatt aagcctggtg gcactgacgc atcatatcag
    31681 tattgcgccg ggtaatatgt tttctaccgg tgaaaactgg tcacgttttt ttcgttttaa
    31741 taccgcgtgg cagtggggag aacgtgaaga acaggcggta aaacaattag gcaaacttat
    31801 tcaagaacgg ctgtaatagc gtttaattta attcctctca ggttgggtaa tatgaatttc
    31861 gaatagcagt catatatcct aactccttgt ctatactcca gaagataacc ttacagacgg
    31921 cataatgcgc ggtagctcac aacctgatta aattttctca gggccgaagg tgtgcctgca
    31981 agccgccgtc tatggttaaa caaggagata tttttacggc acggcggctg aacaattaat
    32041 tacgacagga gtaagacctt atgagcaaga catttgcccg cagcagcctg tgtgcgctca
    32101 gcatgacaat aatgaccgct cacgccgccg aaccgcctac caatttagag aatccggaag
    32161 ggcgactgga tattatcgcc tggccgggat acatcgaacg cggacaaact gataaacagt
    32221 acgactgggt aacgcaattc gaaaaagaga caggctgcgc ggtgaatgtt aaaaccgccg
    32281 cgacttccga tgaaatggtc agtctgatgg ccaaaggggg ttacgatctg gttacggcat
    32341 ccggcgatgc ctcgctgcgt ttgattatgg gcaaacgcgt gcagccaatt aataccgcct
    32401 tgattcccaa ctggaaaacg ctcgatccgc gcgtggttaa aggcgactgg tttaacgtta
    32461 gtggcaaagt ttatggcaca ccttaccaat ggggtccgaa cctgttgatg tacaacacta
    32521 aaaccttccc gacgccgccg gatagctggc aagtggtttt tgttgagcag aatctgccgg
    32581 acggcaagag caataaaggc cgcgttcagg cttatgatgg ccctatctac attgcggacg
    32641 ctgcgttgtt cgttaaagcc actcagccgc agttgggcat cagtgatccg tatcaactca
    32701 ccgaagaaca gtaccaggcg gtgctgaaag tgctgcgcga tcaacatagt ttgatccatc
    32761 gctactggca tgacactacc gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt
    32821 ccagtgcatg gccctatcag gccaacgccc tgaaagccga aggccagcct gtcgctaccg
    32881 ttttcccgaa ggagggcgtt accggttggg ctgacaccac catgctacat agcgaagcga
    32941 aacatccggt ttgcgcctac aaatggatga actggtcatt aaccccaaaa gtgcagggcg
    33001 atgtggcggc ctggtttggc tcgttaccag tagtgccgca agggtgtaaa gccagtccgt
    33061 tattaggcga gaaaggttgt gaaacaaacg gttttaacta tttcgataaa atcgccttct
    33121 ggaaaacgcc tgtagcagaa gggggcaagt ttgttcccta cagtcgctgg acgcaggatt
    33181 acattgccat tatgggcggt cgctaacttc gctggggtgt tttatgacgt acgcagtgga
    33241 gtttgacaac gtctcacggt tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc
    33301 gataaaagat ggtgagttct tctctatgct ggggccgtcc ggctccggca aaaccacttg
    33361 cctgcgcctg attgctggat tcgaacagct ttccggcggg gctatctcta tttttggtac
    33421 gcccgccagc aatctgccac cgtgggagcg ggacgtgaat actgtcttcc aggactacgc
    33481 gctatttccg catatgtcga ttcttgacaa cgtagcctac gggctgatgg tcaaaggcgt
    33541 gaataaaaag cagcggcatg caatggcgca agaggcgctt gagaaagtgg cgttggggtt
    33601 tgtacagcaa cgtaaaccgt cacaactttc tggtggtcag cgccagcggg ttgctatcgc
    33661 cagagcactg gtgaacgaac cgcgtgtgtt gttgctggat gaaccgctcg gcgcgctgga
    33721 tctcaaattg cgagagcaaa tgcagcttga gctgaaaaag ctgcaacagt ctctcggtat
    33781 cacttttatc ttcgttaccc acgatcaggg cgaagcgtta tcgatgtccg atcgggtggc
    33841 ggttttcaat aatggacgca ttgaacaggt cgattctccg cgcgatctct atatgcgccc
    33901 gcgcacgccg tttgttgcgg gattcgtcgg gacatccaac gtttttgatc cgctaatggc
    33961 cgagaaactt tgtggcatga cgggaagctt cgcactacga ccggaacata tccgcctcaa
    34021 cacccatggc gaaatgcagg ctaatggcac gatccaggcg gtgcaatatc agggcgcagc
    34081 gacccgtttt gaactgaaat tgaacggcgg tgaaaaactg ctggtgagtc aggccaatat
    34141 gacaggcgaa gagctacctg ccacgctcac gcccggacaa caggtgatgg tttcctggtc
    34201 acgtgatgtg atggtggcgc tggttgagga gaggtgaatg gcgatgaacg tattgcaatc
    34261 accttcacgt ccaggtctgg gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg
    34321 gctgtttttg ctgctgcttg gcccgctaat gtggtttggc attgtctatt tcggctcgct
    34381 gctgacgctg ttatggcagg gcttttatac ctttgacgat ttcaccatgt cggtaacgcc
    34441 ggaactgacg ctggcgaata tccgcgcgct gtttaatccg gcgaattacg acatcattgt
    34501 ccgcacgctg accatggctg tggcagtcac tatcgccagc gccattctgg cttttccaat
    34561 ggcgtggtat atggcgcgct ataccagcgg gaaaatgaaa gcgttttttt atatcgcggt
    34621 aatgttgccg atgtgggcga gctacattgt taaagcctat gcctggacgt tattgctggc
    34681 aaaagatggc gtggcgcagt ggtttttaca acatctgggg ctggaaccac tgctgactgc
    34741 gttcctcacg ctaccggcgg tgggaggtaa tacgctgtca acttccgggc tggggcgctt
    34801 tctggtgttt ctctatatct ggttgccgtt catgatcctc ccggtgcagg cggcgcttga
    34861 gcgtttgccg ccgtcattgt tgcaggcgtc ggctgatctt ggcgcacgtc cacgacaaac
    34921 ctttcgctat gtggtgctgc cgctggcaat cccgggtatt gccgctggct ctatctttac
    34981 cttctcactc acgctgggcg attttattgt cccgcagctg gttggccctc caggatattt
    35041 tatcggcaat atggtttatt cccagcaggg ggcgattggc aatatgccga tggcggcggc
    35101 attcaccctg gtgccgattg ttctcatcgc actttacctg gcgttcgtga aacgtctggg
    35161 agcgttcgat gcactctgaa cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg
    35221 tggttttcct acattttccc atcctgatta tcgctgccta tgcgtttaac actgaagatg
    35281 cggcgtttag ttttccaccg caaggcctga cgctgcgctg gtttagcgtg gcagcacagc
    35341 gtagtgatat ccttgatgcc gtgacactgt cacttaaagt ggcggcgctg gcgacattaa
    35401 tcgcgctggt gttagggacg ctggcagctg ccgcgctgtg gcgacgagac tttttcggca
    35461 aaaacgccat ttcgctgtta ctgttgctgc ccattgcgct gccgggcatt gtcactggtc
    35521 tggcgctatt aaccgccttt aaaaccatca atctggagcc aggatttttc accatcgtgg
    35581 tcggtcatgc gactttttgc gtagtggtgg tgtttaacaa tgtcatcgcc cgttttcgcc
    35641 gcacctcgtg gagtctggtt gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct
    35701 tccgctacgt agtgttgccg aatctcagtt cggcgttact ggctggagga atgctggcgt
    35761 ttgccttgtc gttcgatgaa atcatcgtga cgacttttac ggcaggtcat gaacgaacgt
    35821 taccgttgtg gttgctcaat cagcttgggc gaccgcgtga tgtaccggta accaacgtgg
    35881 tggcactgct ggttatgttg gtaacaacct tgccgatcct gggggcctgg tggctaaccc
    35941 gcgaaggcga caatggtcaa taaccactga tacaggaata tgctatgcaa cataagttac
    36001 tgattaacgg agaactggtt agcggcgaag gggaaaaaca gcctgtctat aatccggcaa
    36061 cgggggacgt tttactggag attgccgagg catccgcaga gcaggtcaat gctgctgtgc
    36121 gcgcggcaga tgcagcattt gccgaatggg ggcaaaccac gccaaaagcg cgtgcggaat
    36181 gcctgctgaa actggctgat gttatcgaag aaaatggtca ggtttttgcc gaactggagt
    36241 cccgtaattg tggcaaaccg ctgcatagtg cgttcaatga tgaaattccg gcgattgtcg
    36301 atgtctttcg ctttttcgcc ggtgcggcgc gctgcctgaa tggtctggcg gcaggtgaat
    36361 atcttgaagg tcatacttcg atgatccgtc gcgatccgtt gggggtcgtg gcttctatcg
    36421 caccgtggaa ttatccgctg atgatggctg cgtggaaact ggctccggcg ctggcggcag
    36481 ggaactgcgt agtgcttaaa ccatcagaaa ttaccccgct gaccgcgttg aagttggcag
    36541 agctggcgaa agatatcttc ccggctggcg tgattaacgt gctgtttggc agaggcaaaa
    36601 cggtgggcga tccgctgacc ggacatccca aagtgcggat ggtgtcgctg acgggttcta
    36661 tcgccactgg ggaacacatc atcagccata ccgcgccgtc cattaagcgt actcatatgg
    36721 aacttggtgg caaagcgcca gtgattgttt ttgatgatgc ggatattgaa gctgtggtcg
    36781 aaggtgtacg tacttttggc tattacaatg ccggacagga ttgtactgcg gcttgtcgga
    36841 tctacgcgca aaaaggcatt tacgatacgc tggtggaaaa actcggtgct gcggtggcaa
    36901 cgttaaaatc gggttcgccg gatgacgagt ctacggagct tggaccttta agctcgctgg
    36961 cgcatctcga acgcgtcagc aaggcggtag aagaggcgaa agcgacaggg cacatcaaag
    37021 tgatcactgg cggtgaaaag cgcaagggta atggctatta ctatgcgccg acgctgctgg
    37081 ctggcgcatt acaggatgat gccatcgtgc aaaaagaggt atttggtccg gtagtgagtg
    37141 ttacgctctt cgacagcgaa gaacaggtgg tgaactgggc gaatgacagt cagtacggac
    37201 ttgcatcttc ggtatggacg aaagatgtgg gcagggcgca tcgcgtcagc gcgcggctgc
    37261 aatatggttg tacctgggtc aatacccatt tcatgctggt aagtgaaatg ccgcacggtg
    37321 ggcagaaact ttctggttac ggcaaggata tgtcacttta tgggctggag gattacaccg
    37381 tcgtccgcca cgtcatggtt aaacattaag gataatattg cagatcgtaa gagtatgaga
    37441 tgatcttgcg tactgtcaga gcactgattt ctctgacagt acgtgctgcg tgcaaaatca
    37501 ctacatcaaa taaaccagcc aaatctcata gagataagag taaacaaacc aacagcagca
    37561 aagacattta tcagtactac agttctactg gaaacattca tttttgccac cttttatcat
    37621 tgcccctatg tgtatagtac gggttaagaa aatccgcaat aatactcatt ctgaagatgg
    37681 ataagggcaa gttgctgttt gatgattttt tctcaaactg gtgaaaaaaa cttgatgcac
    37741 gtcaaaaaat gacgcatatt tgcgcgcgtt ttattcatct ggctggacgc ccgtacatgt
    37801 ctctctatca acacatgctt gttttctatg cggttatggc agcaatcgca tttcttatca
    37861 cctggtttct ttctcatgat aagaaacgca tccgtctctt aagcgccttt ctggtggggg
    37921 caacatggcc gatgagtttc ccggtggcgc tgttgttttc tctgttttaa atcttatgca
    37981 ctggcttgac ggcaccacgc agacttatat catttggatg aatcgataaa tttcacaagt
    38041 ggctaaggag aaagtatgtc gcatctggat gaagtcatcg cgcgcgtgga tgccgcgatt
    38101 gaagagagcg tcattgccca tatgaacgaa ttattaatcg ccctgagcga tgacgcggag
    38161 ttaagtcggg aagatcgcta caccctgcag cagcgtttgc gcaccgctat tgctcatcac
    38221 ggtcgcaaac ataaagaaga tatggaagcg cgccacgaac agttaaccaa aggcggtacc
    38281 atcctctgat taaaatgagc gtctggccac cagccaggcg ccaaccacaa gtaaaattgc
    38341 gccacaaatt gggccgatca acgcccgaag tggtacgcca tggctgcgga agatatccag
    38401 caccagaccg ccaatcagct ggctggcgac cagtactgca atagttgtcg ccgctcccac
    38461 attctgatat ccgctgatac tggcgaagac aaaaaacgat cccagcaagc caggtataag
    38521 tgtccaccag cgcacgctgg acaccagttc gccaaacccc gccatgcctt gtttaaacca
    38581 taaaatagaa acaaagagca caatccccac cagtgagttc agcaacatgg cgatgagaat
    38641 ggtagaggag gtctgggtga tgcgcaccat cagcgtgttc tggactacca gaccaatccc
    38701 ggctgcaatc aaaaaggcaa gggtaagtga ctgattcatc caatcgcgtc cggttcagtg
    38761 cgctcgtcga gttgcaactg cataaatgtc agatccagcc agcgcccaaa tttagtgcct
    38821 acctgcggca tttgcgcggt gacgacaaat cccagcgact ggtgcagatg cagcgaggcc
    38881 tgattttgcg attcgatccc ggcgaccatg acatgcttcc cgcaatcccg cgcttcatca
    38941 attaatcggc ttaacaattt acgccccaga cctttcccct gatgatcggg atggacataa
    39001 accgaatgtt ccacggtatg gcgaaaacca tcaaaactac gccagtcgcc aaacgaggca
    39061 tatcccgtca ctacgccatc ttcttcactc accagcaccg gataacctgc tatggtccgc
    39121 gcttcaaacc aggcaatgcg gttatccgca tccaccgttt ggtcattcca gatagccgcc
    39181 gtatacaaca cggcgtggtt ataaatttcc gcaattgcag cacagtcggc tttgcgggca
    39241 aaacggatgg acatgtctgg cctcgataaa ataatgatga tgatgttatc aatactatcg
    39301 aaaacagccc gaagcaacgg attcctctat gaaattattg cgtgttgcag aggggatgtg
    39361 acggctgcaa acaaaaccaa tcctgtgagc ctgctactat tgatggattc atgccaaaag
    39421 cacaggagag cattatgggg caacaaacgc agcgtaaccg atgttgggtt ctgtcctcgc
    39481 gtccgcatgg cgcgccggtt ccttacgtct ggtttcggta aatgcgctct ttgtcgcacc
    39541 agcgcaagca tttacagttc cggaagtggg aaaacgtcaa ttaaatgaaa tcaatcggat
    39601 ccgctacgaa ctggagttaa tggcagttgc tctggctgtc gaaaacctca ctatgcaaaa
    39661 aaacgttgct atgttgtgcc agcaatacag ccgataaata aaatacgccc ggcaaatacc
    39721 gggcttaaag ataattactc aaatcgccac gcaatattta tgccgacacc ataatttcgt
    39781 ccgggcgcag gttcgtaata tcgcccgttt gactcattga caatgacaga accaacgtat
    39841 tctttatcga ataaattatc gacacgacca aataaatcca cggttaaatt gtggtaattg
    39901 tatttatacc cggtgaataa gccgacgagt gtataagaag gcgctttggc cgtattttca
    39961 tcatctgcca taatatcgcc catataacgc gcttccgtgc ctgcatacca accatcttcc
    40021 ggtatataac ctatcgacgc aaagcccata ttacgggcga tccctggcat ccggttaccg
    40081 ttacaatcct gttcattgca gacattgctg cgataggtcg catcaagcca ggtccaggac
    40141 gcttttacgc gaaaatctcc cgcgaaacgt tgatcccatg ccagttcagc gccttgacga
    40201 cgggtctttc cggcattttt gtaagtcgta cgcccaccgc tactgctatc gacaacaatt
    40261 tcatcatcgg tgtcggtctg gaacaatgcg agactaagca gtccatcacc aatacgcgtt
    40321 ttactgccga tctcaattgt atcgttggtg gagggtttta aaccaaagtt cataccgctt
    40381 tgtccatcag cgcgataaga cagttcatta atcgtcggcg tttcaaaacc gcgcccggct
    40441 gccagataga cattccaggc atcggtcatt gcatatttta acgatccggc aggtagccat
    40501 ttgtgataac tggcatcacc gctgtcatcg ccgttacccg gagtaacgta atgatcgttg
    40561 gaatcaaacc acacggagct gtagcgcacg ccagcatcca gcgaaagttt gtcggtcagt
    40621 tgccactggg tttgtaaata gggatcgacg ttccacatca aattgcgttc gtcgcggcgc
    40681 aactcacctt tttgcccgta ttccggcacg ccgcgattca ggcggaagtt attgtaaccc
    40741 ttgcggtttt cactcatgtt ttcgtagttc aggccggttg tgaacgtgac tggaacgccc
    40801 agttcaccac ggtgtgtcca gcggctgtct atcccctggt aatggcgttg cagggtaatc
    40861 acgccgcccg catgtgacgg gttaagttgc ggggccatgg ggattgactg gtactgggtc
    40921 gtttctcgct ctccggcata catcatcaca ctcatatcat cttgtgcgct caggctacgc
    40981 tcatagcgca acccagcctg agtttgcttg atggtttttc gcatgtcgta ctgttctgca
    41041 cgaggcgctt gttgtggatt cgccttccat tctgcttcgg ttagcccacc tgggtcatct
    41101 gctttgatat ccacactatt gaaaatcagg cttaatttgc tggcgtcatc aatgcgtacg
    41161 cccagtttgg cattggctaa atttttctgt gcgccactat ggtcacgata gccgtgggtc
    41221 gtaaaacgtg tggttgcgac ggtgtaatcg acatcgccgg gttgtgtgcc gtcgcccgtt
    41281 gcgcccgttg ctttcagccc atagcgccag ctgccaaaac tgccgtagta actactggct
    41341 tcaatggttg gtggctgttg tccggtctgg gtggtgacat tcattacacc gccagacgcg
    41401 ttgccataca gagcagagaa ggggccacgc agcacttcca cattttgcac actgcttaaa
    41461 tcgatgttag atgtctgccc ttgtccgtcg ggcatggtag cgggaatacc gtccacatac
    41521 aggcgaatac cgcgaattcc gtaagtggag cgggagccaa atccgcgaat cgacagctgt
    41581 aaatcttgcg catagttctg tcggttttgt acctgtaaac caggcactcc ggtcagtgat
    41641 tcagacaagt taatgcgcgg tgttgccagg cgcatctcct cgccatccac cacgctcacg
    41701 gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg cactgacaat catggtctgt
    41761 tcatcagcgg caaaaacaac gggggaaagg acaagcagtg cgggcaaaac ggtctgtcgg
    41821 acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg gaaaacaccg tcatggtaat
    41881 aaaattgtaa atttatggaa aacaaaacgg cacaatacgt taagtaattg agaaaattgt
    41941 agtcataacg gtaagaaatg ctccacattt gagaaaataa tgattaccat tcccatttat
    42001 aacaagggcg taacgatgat tacgcttagc gaagcattgt gaagcagcaa aaatatcggt
    42061 tcatcaaagg gagtcgtcat gcatttacgt catctgtttt catcgcgcct gcgtggttca
    42121 ttactgttag gttcattgct cgttgcttca tcattcagta cgcaggccgc agaagaaatg
    42181 ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt atagccagca agaaaacgcg
    42241 ctgtggattg ccacttcgca aagccgcaaa ctggataaag gcggcgtggt ttatcgtctt
    42301 gatccggtta ctctggaagt gacgcaggcg atccataacg atctcaagcc gtttggtgcc
    42361 accatcaata acacgactca gacgttgtgg tttggtaaca ccgtaaatag tgcggtcacg
    42421 gcgatagatg ccaaaacggg cgaagtgaaa ggacgtctgg tgctggatga tcgtaagcgc
    42481 acggaagagg tacgcccgtt gcaaccacgt gagctggtag ctgacgatac cacgaacacc
    42541 gtttacatca gtggtattgg taaagagagc gtgatttggg tcgttgatgg cgagaatatc
    42601 aaactgaaaa ccgccatcca gaacaccggt aaaatgagta ccggtctggc gctggatagc
    42661 aaaggcaaac gtctttacac cactaacgct gacggcgaat tgattaccat cgacaccacc
    42721 gacaataaaa tcctcagccg taaaaagctg ctggatgacg gcaaagagca cttctttatc
    42781 aacatcagcc ttgataccac caatcagcgt gcatttatca ccgattctaa agcggcagaa
    42841 gtgttagtgg tcgatacccg taatggcaat attctggcga aggttgcggc accggaatca
    42901 ctggctgtgc tgtttaaccc agcgcgtaat gaagcctacg tgacgcatcg tcaggcaggt
    42961 aaagtcagtg tgattgacgc gaaaagctat aaagtggtga aaacgttcga tacgccgact
    43021 catccgaaca gtctggcgct gtctgccgat ggcaaaacgc tgtatgtcag tgtgaaacaa
    43081 aaatccacta aacagcagga agctacccag ccggacgatg tgattcgtat tgcgctgtaa
    43141 tagatatggc gagggtgaaa acggaaagaa ttgcctgact tttatcacgc cgcatccggc
    43201 accttcgtca gtagtctgaa tttgcccggc acgtagccgg gcaattttgc aattaactcg
    43261 ccgtttcagg cttaaacaca atgtcctgtt tttcttcatc ttcctcgacg actggcgcag
    43321 tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca gcctataacc agcagaatac
    43381 cgataatcgg cagcgccgcg atggtataag tcccgttcgg gtaatcgaac gccatcagca
    43441 ccaggacact cagtaaaaac agtaatgtca gccaggaagt gaagggcgcg ccaggcagtt
    43501 taaaactgac atccgctgct ttgccttctt taatcgcttt acgcaggcgc atctggcaaa
    43561 cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc gaagttcaac acaatctcaa
    43621 atacgcgtga cggcaccaga tagttgagga atacgccgac gacatacaca accagtgtcg
    43681 ccagaatccc ggcatacggc acatgctgac gactcatttt cgccataaaa ctcggtgcgg
    43741 aaccgcccat cgccatcgag cgcagaatac gcccggtgca gtacagacct gaattcaggc
    43801 tggagagggc agcggtcagc accacaatgt tcataatgct gcctatatat ggcacaccca
    43861 gtttagagaa aaacgtcacg aacggacttt gccccgcctg atatgcgctc cacggcaata
    43921 acataaccag caacaccacg gagccgacgt aaaacagacc aatacgccaa atcacactgt
    43981 taatcgcttt aggtaccatg gtctgcggat ctttacattc tccggccgct gtacccacca
    44041 tttcaatgga ggcaaaagca aacactacgc cctgaatcaa caccagcgca ggcagcaaac
    44101 cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa gccagtggtg ttgccatcca
    44161 gcggctgacc gctaccgagg aacactgtac ccacgaccaa aaaggttaca atggcgagca
    44221 ctttaataag cgcaaaccag aactccatct ccgcaaacca tttcacaccg atcatattca
    44281 tggtgccaac gatggtcagt gcagcgagcg caaagaccca ctgcggcacg ccgccaaacg
    44341 cgccccagta atgcatatac agagcgacgg cggtaatatc aacaatcccc gtcatcgccc
    44401 agttgatgaa gtacatccag ccagcaacat aagcggcctt ctcacccaaa aactcacggg
    44461 cataagagac aaaactgcca ctggaagggc ggtgtaacac cagctcaccc aatgcacgca
    44521 aaataaaaaa cgaaaacagg ccgcagatca aatagaccag tgccagtgcg ggccccgcca
    44581 tttgcagtcg cgcccccgcg cctaaaaaca aacccgtacc aatcgcgccg ccaatggcga
    44641 tcatctgtac ctggcgattg cccatcgctt tgtgataccc ctcttcgtgg gcattaagcc
    44701 agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtt gtgtttactc attgctctcc
    44761 ctgattgctt taatgaaaaa gtcatataag ttgccatgaa caatgtttat tcatcctggc
    44821 gatgacaaga ttcaccatta ttctttatgg gtataactgt cggcgcagaa tcctaccttt
    44881 tatcatgtaa cgatgcaaaa taatcccgct ataaatatgt ggggataaag tcggctggcg
    44941 acaaaatagc aacctgatga aattatgacg gaaacaacca ctattggttt gatgatacct
    45001 gactgcagtc ctactcttgc gccactcgtt cgtcgctctg aacgccagtg gttgcagtgg
    45061 acactggcgt ggttggctca atgaatagtg aggggctagc acatgatggg tttcaggggt
    45121 aatacgccaa ataccaatac aattgctgat taaatgagcc aattgccgac attacagaaa
    45181 aggctaaaaa atgtaagagg catgtagggt gataaacgcg ctattgccca acaggagttt
    45241 ctggaattag tcctgttcca tg
//
LOCUS       DACADA010000019        44648 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.019, whole genome shotgun sequence.
ACCESSION   DACADA010000019 DACADA010000000
VERSION     DACADA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.019"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(144..1034)
                     /gene="metF"
                     /locus_tag="ISB17_001059"
     CDS             complement(144..1034)
                     /gene="metF"
                     /locus_tag="ISB17_001059"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAP1979014.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(1416..2969)
                     /locus_tag="ISB17_001060"
     CDS             complement(1416..2969)
                     /locus_tag="ISB17_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP1979015.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3102..4232
                     /locus_tag="ISB17_001061"
     CDS             3102..4232
                     /locus_tag="ISB17_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP1979016.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(4196..6628)
                     /locus_tag="ISB17_001062"
     CDS             complement(4196..6628)
                     /locus_tag="ISB17_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAP1979017.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6631..7791)
                     /gene="metB"
                     /locus_tag="ISB17_001063"
     CDS             complement(6631..7791)
                     /gene="metB"
                     /locus_tag="ISB17_001063"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP1979018.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            8068..8385
                     /gene="metJ"
                     /locus_tag="ISB17_001064"
     CDS             8068..8385
                     /gene="metJ"
                     /locus_tag="ISB17_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAP1979019.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            8445..9053
                     /locus_tag="ISB17_001065"
     CDS             8445..9053
                     /locus_tag="ISB17_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAP1979020.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(9114..9326)
                     /gene="rpmE"
                     /locus_tag="ISB17_001066"
     CDS             complement(9114..9326)
                     /gene="rpmE"
                     /locus_tag="ISB17_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAP1979021.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            9529..11727
                     /gene="priA"
                     /locus_tag="ISB17_001067"
     CDS             9529..11727
                     /gene="priA"
                     /locus_tag="ISB17_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP1979022.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            11883..12908
                     /gene="cytR"
                     /locus_tag="ISB17_001068"
     CDS             11883..12908
                     /gene="cytR"
                     /locus_tag="ISB17_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAP1979023.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            13000..13959
                     /gene="ftsN"
                     /locus_tag="ISB17_001069"
     CDS             13000..13959
                     /gene="ftsN"
                     /locus_tag="ISB17_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAP1979024.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            14052..14582
                     /gene="hslV"
                     /locus_tag="ISB17_001070"
     CDS             14052..14582
                     /gene="hslV"
                     /locus_tag="ISB17_001070"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAP1979025.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            14592..15923
                     /gene="hslU"
                     /locus_tag="ISB17_001071"
     CDS             14592..15923
                     /gene="hslU"
                     /locus_tag="ISB17_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAP1979026.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            15990..16916
                     /gene="menA"
                     /locus_tag="ISB17_001072"
     CDS             15990..16916
                     /gene="menA"
                     /locus_tag="ISB17_001072"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP1979027.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            17009..17494
                     /gene="rraA"
                     /locus_tag="ISB17_001073"
     CDS             17009..17494
                     /gene="rraA"
                     /locus_tag="ISB17_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAP1979028.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(17579..17824)
                     /gene="zapB"
                     /locus_tag="ISB17_001074"
     CDS             complement(17579..17824)
                     /gene="zapB"
                     /locus_tag="ISB17_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAP1979029.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            18249..19094
                     /gene="glpF"
                     /locus_tag="ISB17_001075"
     CDS             18249..19094
                     /gene="glpF"
                     /locus_tag="ISB17_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAP1979030.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            19117..20625
                     /gene="glpK"
                     /locus_tag="ISB17_001076"
     CDS             19117..20625
                     /gene="glpK"
                     /locus_tag="ISB17_001076"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP1979031.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            20796..21806
                     /gene="glpX"
                     /locus_tag="ISB17_001077"
     CDS             20796..21806
                     /gene="glpX"
                     /locus_tag="ISB17_001077"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP1979032.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            21903..22649
                     /gene="fpr"
                     /locus_tag="ISB17_001078"
     CDS             21903..22649
                     /gene="fpr"
                     /locus_tag="ISB17_001078"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAP1979033.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(22654..23082)
                     /gene="uspD"
                     /locus_tag="ISB17_001079"
     CDS             complement(22654..23082)
                     /gene="uspD"
                     /locus_tag="ISB17_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAP1979034.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(23109..23408)
                     /locus_tag="ISB17_001080"
     CDS             complement(23109..23408)
                     /locus_tag="ISB17_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAP1979035.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(23620..24060)
                     /locus_tag="ISB17_001081"
     CDS             complement(23620..24060)
                     /locus_tag="ISB17_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1979036.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            24161..24760
                     /locus_tag="ISB17_001082"
     CDS             24161..24760
                     /locus_tag="ISB17_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAP1979037.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            24868..25635
                     /gene="tpiA"
                     /locus_tag="ISB17_001083"
     CDS             24868..25635
                     /gene="tpiA"
                     /locus_tag="ISB17_001083"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP1979038.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(25690..26445)
                     /gene="cdh"
                     /locus_tag="ISB17_001084"
     CDS             complement(25690..26445)
                     /gene="cdh"
                     /locus_tag="ISB17_001084"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAP1979039.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(26552..27541)
                     /locus_tag="ISB17_001085"
     CDS             complement(26552..27541)
                     /locus_tag="ISB17_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1979040.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(27861..28823)
                     /gene="pfkA"
                     /locus_tag="ISB17_001086"
     CDS             complement(27861..28823)
                     /gene="pfkA"
                     /locus_tag="ISB17_001086"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP1979041.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(29004..29906)
                     /gene="fieF"
                     /locus_tag="ISB17_001087"
     CDS             complement(29004..29906)
                     /gene="fieF"
                     /locus_tag="ISB17_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAP1979042.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(30055..30555)
                     /gene="cpxP"
                     /locus_tag="ISB17_001088"
     CDS             complement(30055..30555)
                     /gene="cpxP"
                     /locus_tag="ISB17_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAP1979043.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            30705..31403
                     /gene="cpxR"
                     /locus_tag="ISB17_001089"
     CDS             30705..31403
                     /gene="cpxR"
                     /locus_tag="ISB17_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAP1979044.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            31400..32773
                     /gene="cpxA"
                     /locus_tag="ISB17_001090"
     CDS             31400..32773
                     /gene="cpxA"
                     /locus_tag="ISB17_001090"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAP1979045.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(32944..33618)
                     /gene="yiiM"
                     /locus_tag="ISB17_001091"
     CDS             complement(32944..33618)
                     /gene="yiiM"
                     /locus_tag="ISB17_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAP1979046.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSVAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(33767..34750)
                     /gene="kdgT"
                     /locus_tag="ISB17_001092"
     CDS             complement(33767..34750)
                     /gene="kdgT"
                     /locus_tag="ISB17_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAP1979047.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(35010..35630)
                     /gene="sodA"
                     /locus_tag="ISB17_001093"
     CDS             complement(35010..35630)
                     /gene="sodA"
                     /locus_tag="ISB17_001093"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAP1979048.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQAYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            35915..36949
                     /gene="rhaT"
                     /locus_tag="ISB17_001094"
     CDS             35915..36949
                     /gene="rhaT"
                     /locus_tag="ISB17_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAP1979049.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(36946..37884)
                     /gene="rhaR"
                     /locus_tag="ISB17_001095"
     CDS             complement(36946..37884)
                     /gene="rhaR"
                     /locus_tag="ISB17_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAP1979050.1"
                     /translation="MVFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(37868..38704)
                     /gene="rhaS"
                     /locus_tag="ISB17_001096"
     CDS             complement(37868..38704)
                     /gene="rhaS"
                     /locus_tag="ISB17_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAP1979051.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHCVLQQVRQLVAQLEQQEEGNDLPSTASREILFMQLLLLLRKS
                     SLQESVENSASRLNLLLAWLEDHFADEVNWDAVAAQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            38992..40461
                     /gene="rhaB"
                     /locus_tag="ISB17_001097"
     CDS             38992..40461
                     /gene="rhaB"
                     /locus_tag="ISB17_001097"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP1979052.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            40458..41717
                     /gene="rhaA"
                     /locus_tag="ISB17_001098"
     CDS             40458..41717
                     /gene="rhaA"
                     /locus_tag="ISB17_001098"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP1979053.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLLDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            41984..42808
                     /gene="rhaD"
                     /locus_tag="ISB17_001099"
     CDS             41984..42808
                     /gene="rhaD"
                     /locus_tag="ISB17_001099"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP1979054.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            42818..43132
                     /gene="rhaM"
                     /locus_tag="ISB17_001100"
     CDS             42818..43132
                     /gene="rhaM"
                     /locus_tag="ISB17_001100"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP1979055.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(43173..44567)
                     /locus_tag="ISB17_001101"
     CDS             complement(43173..44567)
                     /locus_tag="ISB17_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP1979056.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        10960 a        11668 c        11383 g        10637 t
ORIGIN      
        1 agggttttag atttgtatcc ttcaagtaat gagggagaaa acaaggctga taaccttatt
       61 ttcccgccct catttcgagg cagcattttg tgctctgttt aaaatttgtg aacactgtgt
      121 gattttcaca aaaggcacaa tacttataaa ccaggtcgaa ctcccagtgt atggcaaatc
      181 gcataactca tttcagcgcg gttaagcgta tagaagtgga aatctttcac tccttcacgg
      241 cttaaaatct tcaccatatc catggcgata ttcgcgccca ccagcttgcg ggtttcagca
      301 tcgtcatcca ggccgtcgaa catttgtgcc atccacgccg gaatacgcac gttggtcatg
      361 tcggcaaatt tcttcgcctg tttaaagttc gaaaccggca aaattcccgg aatgatttcc
      421 acatcaatgc ctgccgagac gcagcgatcg cgaaaacgca ggtagctttc gacatcgaag
      481 aagaactgag taatggcgcg gttggctccg gcatccactt ttcgtctcag gttgagtaaa
      541 tcggcctggg cacttttcgc ttctggatgt acttccggat acgccgccac ggagatatcg
      601 aaatctgcca cttcttttaa cagcgtcacc aggtcagaag catacatttc tggcttacca
      661 cttcccggcg gcaggtcgcc acgcagcgcc acgatatggc gaataccgtt attccagtag
      721 tcgcgtgcaa tgatccgcag ctcggcaggc gtcgcatcaa tgcaggtaag atgcggtgcc
      781 gcttccaggc cggtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg
      841 ccggagtttg cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg
      901 atggagttcc acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ttcgaacgaa
      961 acgttaatct gcccctggac ttctgccaga ctctgattca gggcatcccg ctggctggcg
     1021 tgaaaaaagc tcatacctta cctcatcaat cgcttatcgt tatatgttgt gttgtgcaca
     1081 tctatccgtt tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa
     1141 aaataaccaa tgatggtgag gaaaaatcag ggaagatgaa aaaaactcat cttaattaga
     1201 ggcgataaaa gatttctcta tagcgcaatc cacctcgcac gttttgactc atattctgac
     1261 aacacagcga cacctgggct aattcgaaaa acaacgtcct gccaccggat ggtgatgtta
     1321 aggcatctga ttcggcctgc gccctcgcgt aggccgaata caggatctgc gcaatcaacg
     1381 ggtaacaaac agttgagtca attcagtgcg cacttttatt tgatatcctt cacgcgcata
     1441 ccgttgagct gttcatccgt aatggtgttg cccgctttga agtaatcaac caccgcgtga
     1501 taaacgtaat aaccgcccgt tgtgttacgc gctttccctt cggtaaaggc ggtaaaacca
     1561 tctccaccat cagcaaggaa actctgagta gcaatgtgat aaaccgtcgc atcttcaatg
     1621 ggtttgccat tcaaagtcag tgtgattacc cgctgaccaa ccggcctgtt gctgtcgtac
     1681 ttcatttcca ggcctttcga tacctgtaaa acgccattgc ttaaacttgc gccgtgttcc
     1741 atcagactgc gtaattgttt acccatgagt tccatcgtca ccagttcgtt ggggaacgga
     1801 aacgtactga tgacgccgcc catcgtgatc gcccctgcgg ggatctcatt gcggatacca
     1861 ccagagtttg ttaacgccag ttgggtgttt ttacccgccg ctaccaataa agcgtcagcc
     1921 gccaggttcc cgagggaagc cgattcacca taggcacgtt ttaattcaac cggcgattgc
     1981 gccaccgttt gttgcaccac ttcatcaagc tttttattcc agccgtcgat cacctgtttc
     2041 gtttgcggat cgggcttcca ctcgtcagcg taaatggttt taagctcgaa gtttttcacc
     2101 gtaaaattat gcggcttctc tttgtagtcg agaaccagtt tgcccacatc aatcccgccg
     2161 ctgtcagtgg agaggatgag cgtattgccg actttaatcg gttccggcgt acccacatgt
     2221 gcatgtccgg tgatcaaaat atccagccct ttcacctgac ttgccgtctg aatatcttta
     2281 tccaacgcgc gacgcacatc tgtgcctccc atactggact ggcgggctgg aacaccttcg
     2341 tggatcaggg cgacggtcaa atcaaccttg cctttgagtt catcgatata acgttgtagc
     2401 catttaattt catcgcgcgc ttcaataccc acgcgcgtgg cagcagatac cgtatcatta
     2461 aaggcaaata ctccgtgcaa accaataacg cctattttca caccgtcttt ttcgatgatg
     2521 gtatagggct tatcccagaa tgatttactg ctgttctgat aaaaaatatt gccctgcaca
     2581 atagggaatt ttgcctggct caactgtaat aatgtgttgt cccagccgtg gtcaaattca
     2641 tggttgccta ttgtgacggc atcaaacggc atagtattca taatatcgat aatagcctta
     2701 cctttggtca ggctgctgat atatggcccg gtaaaatagt cgcccgcatc aaaaaaccat
     2761 gtcgctttat tttttgcttt ttcttgctta accagcgtag tgatatttgc ccagccgcca
     2821 atatcccgtt taccgtcagc gatccacggc actttataag gctcgacatg tgcatggagg
     2881 tcgttggtat aaatgatggt gacatcttta gcgcaagccc agaatggcag tgttaatgcg
     2941 atacccgcag caagtatttt aattttcata aacagtccct ttcagtgaga taaaggattg
     3001 ctacattacg ttctgaaaga cttatttatg tgagagtaat cacatccgca ggggatagtt
     3061 gccggacaaa atcaacgacc ttgtgaaatc gttggtgata tatgtcgact ctgtttacga
     3121 ttaatgcctg taagtcgttt ggttgccgta atttagggca gccaacatcg acagattata
     3181 gctggcccga ctatcgactg ggttatccgg cactgcactg tcgggcatgt ggcagttacc
     3241 cgccgttgtt tgacgagcaa caatttcgtg actggttgac agttcactta tctacttttg
     3301 caacagagaa aggccatttt tgcccggtat gttatggcac agagacgatt tgttatggtc
     3361 acaatccaca gggttcgcaa cgcattcaat gccgtaactg caaaaaagta tggacaccca
     3421 aacaatatca gaaagaaata acgactcccg aaataatcga aacggttgct ttgctcgtgc
     3481 catttcaggg agtcagcagc gggcagaaac tgtatgtttt aatcagtttt gatgctcttc
     3541 gcggtaatat tcttcatctt tctactaatt acacgcagca tcaggctgga gaaagtctgc
     3601 attatcgata cagaggaaat gcagaaccag agttacacga caataatatt gtacaacgcg
     3661 ttgatatgcg cgaagcacaa tttctacgcc gcagtcagtt tgatgaaatt cagtatggca
     3721 gcgcagcgct caagcgcaat gccaaaggcg ctattctgcg tccggttatc actgcacacg
     3781 ggcatttcag agtactgaat attttattcc ctacagtaaa aacacatgta atttcacacg
     3841 agtgtttttt aagaggtgcc attattacgg cctgggctga tctgtttcgc caacagcaag
     3901 gtgaaatttg gtttattgaa gaggaaatag ctgacgatac cgataatatg ccctggcgtt
     3961 ttcagggaaa aacttatcac ggctggtgga aaaatcagtg gcaactctgg gttcagggaa
     4021 aaaatcgtaa aatggtatgt gcattaacgg gcggaaagag tagcaaagca gaaatgctct
     4081 ctttagcaac cagcagacat tttatcgact ggctacataa acaagcagta tttactcaca
     4141 gcgccccgct ttccgcaggg cgcgttacgc aaatactgct ttctctggct caggattaca
     4201 acaactgcgc cagtcggtta atatccgact gaatcgcccc ggcggtgacg tcgcgccctg
     4261 cacctggccc gcggatcacc agcgggttgt cgcgatacca gcggctttcg atggcaaaga
     4321 cgttatcgca tggcagtagt gatgccagcg gatggtcttc acgcaccgct tccacgccca
     4381 cacgcgcttt gccattggca tcgaaacgcg ccacgtagcg cagcaccagc cccatttcgc
     4441 gggccgcttc cagtcgttgc accatctgct cgttaagttc atcgccattt tcaaagaaat
     4501 ggtcgatgct gccgccttcg caatgggcag gcaccagtga ttcaacacgt acctggtccg
     4561 gttcaatgtt gtaacccgct tcacgcgcca gaatcaccag cttgcgcatt acgtctttgc
     4621 cggaaaggtc atcacgcggg tcaggttcgg ttaagccttg ctgccacgcc tgatccacca
     4681 gttcggtaaa cggcacgcta ccgtcgaatt gcaggaacag ccaggagagc gtgccggaga
     4741 agatcccact gatcgacaaa atggtatcgc cgctgtcgat cagatcgcgc acggtatggt
     4801 tgatcggcaa acccgcaccg acggtggcgt tgtacagcca gtgacgcccg gttttttcga
     4861 aggcgtcgtg gatctggcga tatttattgc tgtcgcttgc tcctgccagt ttgttggcac
     4921 tgatgacgtg gaaaccgtgg ctggcgaaat caagatactg gtcagcgagc tgctggctgg
     4981 cggtaacgtc cagcaccact aaatcatcat acggatgggc gcgcatccac aggaacagcg
     5041 actcttcatc ctgctcgacc gcttcatcgt tgaagaaggc taacgcgcgg ctggcgtcca
     5101 gaccgtcata gctcaacagg ctacggcggc tgtccaccac gcctgccagc acaaattcaa
     5161 agccggtacg tgccgaaagc gtactctgct cacgggcgaa cagttccagc cagcgggaac
     5221 cgatattgcc cttgccgaac aataccaggc cgatgcgttt ttccgcgcgg aagacggact
     5281 gatgcagccc ctgaatcagg ctttcggtcg ggccggtgcg cagtactgcc accaggctga
     5341 tgccgtcatc ggactgccag gtaaattcga ccggctggcc tttcagttgc tgccagaagc
     5401 ggtggcaatg cagcgggtta cgggtgacgc ccgccccaac catcgccacc agcgccagcc
     5461 cctgacgcag gcgcagttcg ccaggtaatc ccgcttcgtc gaggattttc agcgcactgt
     5521 cggccacttc tgaggtgtag caaaattgca gcaactggcg atcgttatgc acgccaaccg
     5581 ccagtggacg tacctgagca cgtttcagga tctggtcgat ctctttatgc gccagtttga
     5641 aatcctggct ggcgggcacc tgaaactcaa tcaagcagac atcatcatgg ctggtgacaa
     5701 tacgcgcacc agtaccggag gccagcacgc gttcaatgcg cgtggaacct tgatccggcg
     5761 tgtagctaca gcgcagttgc aggtcgattt cgctgccaga aacgggctgt aaagtacggg
     5821 catgaagaac gggagccgcc agacgcgcca gttcgctggc ttcatccaga cgcagcaacg
     5881 gcagcaggca ggcatctttc actttacgcg ggtcggcgct atataccccg gcgacgtcgc
     5941 tccagatggt tacgcgagaa acacccgcca gcgcaccgat ttgtgtcgcg gaatagtcgg
     6001 aaccgttacg ccccagcagc accgtttcac cggcgttgtt acggctgata aacccggtca
     6061 ccaccagacg tttgcccgga tgttgtacca gtagctgttg cagcaacggg taagaaagcc
     6121 cttcatcaac ctgcggttgc gcagcgcgtt cagcgcgtaa aaactcgcgg gcatcaagcc
     6181 aggccgatgg cagcccttgt tgattaagca ccgcagacat caggcgcgcc gaccacactt
     6241 caccgtggcc caccacttcc gcatacaccg catcgttaat accgctgtcg agcaacgccg
     6301 ccaggcgctc aagatcgctg acaaaagcgc taatgaggtt atcggcttct tcggcgggta
     6361 gcaggccgct aatcagatcg cactgataac gacgtaatgt ttgttgaacc tgatgcgcag
     6421 agagacgatc ggtctggctt agtttcaacc agttaatcaa ctggttagtg gtgctaccgg
     6481 cggcggaaac caccatcata tcgtcaggct gagagtactc cgccataatg cccgcgacac
     6541 gcaaataaca cttcacatca gccagactac tgccaccaaa tttatgcagt tgacgacctt
     6601 tcgcccctgc ctgcgcaatc acactcattt ttaccccttg tttgcagccc ggaagccatt
     6661 ttccaggtcg gcaattaaat cttcgccatc ttcaataccg gtggagatac gcagcagcgt
     6721 ctcggagatc ccggcggcag cacgcgcttc tggtgccatg cctgcatgtg tcatggttgc
     6781 ggcgtgagag attaaacttt ccactccccc taatgattcc gccagcgtaa acaacgacag
     6841 cccgcccagg aaacgacgca gcgtctgctc atcgccatcc agttcaaaac tcaacattgc
     6901 gccaaagcct ttttgctggc gcgcggcaat ttcatgcccc tgattttccg gcagcgacgg
     6961 gtgatacagt ttcttcacca acggctgggt ttgcagatat ttcacaatcg cctgcgcgtt
     7021 gcgctgcgcc agctccatac gcggcaccag cgttcgcaac ccgcgcagca gcagatagct
     7081 gtcaaacgcg ccgcccgtca cgccaatatt gtttgcccac caggcaagtt cagtgacaac
     7141 gtccgggtct ttagcaatca ctacgcctgc caccacgtca gagtgaccgt tcagatattt
     7201 cgtgcatgaa tgcaacacca gatcggcacc taatgccagt ggattttgta atgccgggct
     7261 taagaaggtg ttatccacca cgctcaccgc cccgacttcc cttgccagat ggcaaatttt
     7321 cgcaatatcc acgacgcgta acaatggatt acttgggctt tctaccagta ccagtttggg
     7381 tttttctgcc agcgctgccc gtaatgcctg ttcatcgctt tgatcaacaa acaacacgcg
     7441 atagcaaccg cgtttcgcca ggctgtcgaa caggcgatag ctaccgccgt agcagtcgtg
     7501 cggcgcaacc agcagatcgc caggtttcaa aaagacggtc gttaccaggt gaatcgcgga
     7561 catgccggta ttagtaagta cggcaccggc accgccttcc agttctgcca gcgcacgctg
     7621 aaccacatcg cgcgttgggt tgccgcgacg cgagtaatca tgcgcacgtg gttcattaaa
     7681 tccggtaaag ttataggtac tggaaagatg gatgggaggg acaacgcaac catactgttc
     7741 gtcgtcattt aacccgctac gcactgcgat ggtggcctgt ttacgcgtca tgtgatgaag
     7801 ttccctgggc tttgtcggtg aaatgtcagg caccagagta aacattgtgt taatggacgt
     7861 caatacatct ggacatctaa acttctttgc gtatagattg agcaaatccc aaatagccgt
     7921 taaaattata tgcattatca cgccgacagg tgcattacac gatgtcacgg taacgcctgt
     7981 acggtaaact atgcgggttt acggtcagta cccacattaa ctgtgtggtc tggtctcaat
     8041 ttattgacga agaggattaa gtatctcatg gctgaatgga gcggcgaata tatcagccca
     8101 tacgctgagc acggcaagaa gagtgaacaa gtcaaaaaga ttacggtttc cattcctctt
     8161 aaggtgttaa aaatcctcac cgatgaacgc acgcgtcgtc aggtgaacaa cctgcgtcac
     8221 gctaccaaca gcgagctgct gtgcgaagcg tttctgcatg cctttaccgg gcaacctttg
     8281 ccggatgatg ccgatctgcg taaagagcgc agcgacgaaa ttccggaggc ggcaaaagag
     8341 atcatgcgtg agatggggat taacccggag acgtgggaat actaaagcgg aaaagagcgg
     8401 cgcggagtta accagccgct tttttcatac atggacgttt aacgatgaaa atcaggctac
     8461 tgatccccgg cctgctggtt tctgtgcctg catttgcctg gcaaccacaa accggcgaca
     8521 tcatctttca gatctctcgc tcatcgcaaa gtaaagcgat ccaactggcg acccattccg
     8581 attacagcca caccggtatg ctggtgatac gcaacaaaaa gccctacgtc tttgaagccg
     8641 tcggcccggt aaaatatacc ccgctgaaac agtggatcgc ccacggtgaa gatagcaagt
     8701 acatcgtgcg tcgcgttaaa ggcgagctaa atgccgaaca acagcaaaaa ctggcgcaaa
     8761 cggcaaaacg ttatctcggt aaaccgtatg actccagctt ctcgtggagc gacgatcgcc
     8821 agtactgttc ggaagtggtg tggaaggtct accagaatgc gctgggaatg cgtgtgggcg
     8881 agcagcagaa gctgaaagag ttcgatctta gtaacccgct agttcaggca aagctcaaag
     8941 aacgctacgg caaaaatatt ccgcttgagg aaacggtagt ttcaccacaa gccgttttcg
     9001 acgcgccaca actcaccacg gtcgccaaag aatggccgct gttttcctgg taatacgacc
     9061 aaaattcagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
     9121 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
     9181 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
     9241 ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg
     9301 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
     9361 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
     9421 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
     9481 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc
     9541 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
     9601 acggttaaag ccgggagtcg cgtgcgcgtg ccgtttggca aacaacagga gcgcatcggg
     9661 gttgtggtgt cagtgagcga tgtcagtgag ctgccgctta acgagctgaa agcggtagtt
     9721 gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcgattact gctctgggcg
     9781 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg
     9841 cgccaggggc ggcctgcggc aaacgcgcct atgtggtact ggtttgccac tgaacaaggt
     9901 caggcggtgg atctgaacag cctgaaacgt tcgccaaagc aacaacaggc gctggcagcg
     9961 ttacggcaag gcaaaatctg gcgcgatcag gttgccacgc tcgaatttaa tgatgccgcg
    10021 ttgcaggcac tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
    10081 gactggcgaa cgaactatgc cgtttctggt gagcggttgc ggttgaatac cgaacaggcc
    10141 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
    10201 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
    10261 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
    10321 tttcgtgaac gttttaatgc cccggtggaa gttctgcatt ccggcctgaa cgacagcgag
    10381 cgtctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtcat cggcacccgc
    10441 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg ttattgatga agagcacgac
    10501 agttcctaca aacagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgc
    10561 gcgcatagcg agcaaatccc aattattctt ggctccgcaa cgcccgcact ggaaacgtta
    10621 tgcaacgtgc agcagaaaaa ataccgcctg ctgcgcctga cccgtcgggc gggcaatgcg
    10681 cgcccggcaa ttcaacatgt gctggattta aaaggtcaga aggtgcaggc aggtctggct
    10741 ccggcgttga tcacccgtat gcgccagcat ttacaggccg acaaccaggt tatcctcttt
    10801 cttaaccgcc ggggttttgc gcctgcgcta ctgtgccacg actgtggctg gattgccgaa
    10861 tgcccgcgtt gcgatcacta ctacacgctg catcaggcac aacagcattt gcgctgtcac
    10921 cactgtgaca gccaacgccc ggtgccgcgc cagtgccctt cctgcggttc cacacacctg
    10981 gtacccgtgg ggctgggcac cgaacagctt gaacagacgc tcgcgccgct gttccccggc
    11041 gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa
    11101 ctggcggaag tgcatcgcgg cggcgcgagg atcttgattg gcacgcaaat gctggcgaaa
    11161 ggtcaccact ttccggatgt gacgctggta gcgttactgg atgtggacgg cgcgctgttt
    11221 tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt cgctggtcgt
    11281 gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct
    11341 ctgttgcaaa cgttgctcta taaaggctac gacgactttg ccgaacaggc gctggctgag
    11401 cgacggatga tgcagctgcc gccatggacc agccatgtga ttgtgcgtgc ggaagatcat
    11461 aacaatcagc acgcgccgct gttcctgcaa cagttgcgta atctgatcct cgccagcccg
    11521 cttgcagacg acaaactgtg ggttctcggt ccggttccgg cactggcccc taagcgtggt
    11581 ggccgctggc gctggcaaat tttgttgcag cacccttccc gcgtgcgatt acagcacatc
    11641 atcaatggta cgctggcgct tattaataca ataccggatt cccgtaaagt gaaatgggtg
    11701 ctggatgtcg atcccatcga gggctaaacc gctcacgatg cgaggcggat cgaaaaattc
    11761 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
    11821 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
    11881 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
    11941 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
    12001 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
    12061 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgacc
    12121 ctttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg
    12181 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
    12241 tcaccaagca aattgatggc atgttgttgc tgggttcaag gctgccgttt gatgccagca
    12301 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaatttgca ccggaactgg
    12361 agctgcctac cgttcatatc gacaatctga ccgccgcatt tgatgcagta aattatttat
    12421 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc
    12481 actaccgcct gcaaggctat gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc
    12541 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag cagagcaatg caacagctgc
    12601 ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg
    12661 cactttctca ggcaaaacgt caggggctga aagtcccgga agacctttcc ataatcggtt
    12721 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc
    12781 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
    12841 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
    12901 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
    12961 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
    13021 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
    13081 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
    13141 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
    13201 cagaaagtga ccggaaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa
    13261 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa
    13321 gtgaaaacgc cggagcaact gacaccagaa caacgccagc ttcttgaaca aatgcaggcc
    13381 gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgagca gacgccggaa
    13441 cagcgtcagc aaacgctaca gcgccagcgt caggcgcagc aattagcgga gcagcaacgc
    13501 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcgcaa
    13561 gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac
    13621 caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
    13681 ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
    13741 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
    13801 ctggcgttcg aaggctttga ctcaaaaatt accaccaaca atggctggaa tcgtgtggtg
    13861 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcgctt gaagatggcg
    13921 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
    13981 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
    14041 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
    14101 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
    14161 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
    14221 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg
    14281 ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
    14341 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
    14401 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgcgcgc
    14461 tgttagaaaa cactgaactt agcgcccgtg aaattgctga aaaggcgttg gatattgcag
    14521 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
    14581 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
    14641 tcatcggcca ggacaacgcc aagcgttccg tggcgattgc tctgcgtaac cgctggcgtc
    14701 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
    14761 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
    14821 cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctatgtcggt aaggaagtgg
    14881 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
    14941 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtactgatcc
    15001 caccggctaa aaataactgg ggacagaccg aacagcagca ggagccgtcc gctgctcgtc
    15061 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatt gagatcgatc
    15121 ttgccgcagc accgatgggc gttgaaatta tggctcctcc gggcatggaa gagatgacca
    15181 gccagctgca gtccatgttc cagaacctgg gcggtcagaa gcaaaaggcg cgtaagctga
    15241 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
    15301 aagagctgaa gcaagatgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
    15361 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
    15421 ttcagcgtga cctgctgccg ctggtagaag gttgcactgt ttccaccaaa cacgggatgg
    15481 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
    15541 acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt tgagctgcag gcactgacca
    15601 ccagcgactt tgagcgtatt ttgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
    15661 cactgatggc aactgagggc gtaaatatcg agtttaccga ctccggtatt aagcgcatcg
    15721 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacata
    15781 ccgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
    15841 ctatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
    15901 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
    15961 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
    16021 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
    16081 ggacggcgct ggcatggtgg caaggtcact tcgatccact ggtcgccctg ctggcattga
    16141 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa
    16201 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
    16261 cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc
    16321 tggcactggt tgcagtggcg tgccatacgc tggccgattt tgtcggtttc ctgattcttg
    16381 gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata
    16441 tcggtttggg tgatatttcc gtactggttt tctttggctg gttgagcgtc atggggagct
    16501 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcaacc gcatgcggcc
    16561 tgctggcaac ggcagtactg aacattaata acctgcgtga tatcaatagc gaccgcgaaa
    16621 acggcaaaaa cacgctggtg gtgcgcttag gtgcagtaaa cgcgcgtcgt tatcatgcct
    16681 gcctgctgat gggctcactg gtgtgtctag cgctgtttaa tctcttttcg ctgcatagcc
    16741 tgtggggctg gctgttcctg ctggcggcac cgttgctggt gaagcaagct cgttatgtga
    16801 tgcgcgaaat ggacccggtg gcgatgcgac caatgctgga acgcactgtc aaaggagcgt
    16861 tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga
    16921 caaatatcaa ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa
    16981 attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat
    17041 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
    17101 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
    17161 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggaggtt ctgttcgccg
    17221 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggcctggt
    17281 catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
    17341 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
    17401 caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
    17461 tattctttca gaagatccgc tggatattga atgataaaaa aggcaccgca aggtgccttt
    17521 tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg aagtgtaatc
    17581 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt
    17641 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
    17701 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
    17761 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
    17821 tcatacctct cctgaattgc aaggcgttga tggataaaaa tcctcgtccc gattaccggt
    17881 gacgccttaa taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc
    17941 gctgtccagc ggcacgcctt gcagattacg gtttgccaca cttttcatcc ttctcctggt
    18001 gacataatcc acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac
    18061 atgcatcatg tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacggagc
    18121 acacacattt taagttcgat atttctcgtt tttgctcgtt aacgataagt ttacagcatg
    18181 cctacaagca tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg
    18241 atccgattat gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta
    18301 ccgggttgtt gatttttttc ggtgtgggtt gcgttgcagc actaaaagtc gctggtgcgt
    18361 cttttggtca gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc
    18421 tgaccgcagg ggtttccggt gcgcatctta atcccgctgt taccattgca ttgtggctgt
    18481 ttgcctgttt cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt
    18541 tctgtgctgc ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga
    18601 ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt
    18661 accctaatcc tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta
    18721 ttctgatggg gctgatcctg gcgttaacgg acgatggcaa cggtgtacca cgcggccctt
    18781 tggctccctt gctgattggt ctactgattg cggtcattgg cgcatctatg ggcccattga
    18841 cgggctttgc catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tggctggcgg
    18901 gctggggcaa tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt
    18961 ttggccctat cgttggcgcg attgtaggtg catttgccta ccgcaaactg attggtcgcc
    19021 atttgccttg cgatatctgt gttgtggaag aaaaggagac cacaactcct tcagaacaaa
    19081 aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt
    19141 gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc
    19201 attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggctg ggtagaacac
    19261 gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagct
    19321 gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt
    19381 gtctgggaaa aagaaaccgg caaacctatc tataacgcca ttgtctggca gtgccgtcgt
    19441 accgccgaga tctgcgaaca tctcaaacgc gatggcatgg aagagtacat tcgtaacaac
    19501 accggtctgg tgattgaccc gtacttctct ggcaccaaag tgaagtggat cctcgaccat
    19561 gtggaaggct ctcgcgagcg tgcgcgtcgt ggtgaattgc tgtttggtac ggttgatacg
    19621 tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct
    19681 cgtaccatgt tgtttaacat ccatactctg gactgggacg acaaaatgct ggaggtgctg
    19741 gatattccac gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact
    19801 aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag
    19861 gcggcgctgt ttggtcagtt gtgcgtgaaa gaaggaatgg cgaagaacac ctacggtacc
    19921 ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg
    19981 accaccatcg cctgcggccc gactggcgaa gtaaactatg cgctggaagg tgcggtgttt
    20041 atggcgggcg cgtccattca gtggctgcgc gatgagatga agttgattaa cgacgcctac
    20101 gattccgaat attttgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggcc
    20161 tttaccgggc tgggtgcgcc atactgggat ccgtatgcac gcggggcgat tttcggtctg
    20221 actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag
    20281 acgcgtgacg tgctggaagc aatgcaggcc gactccggta ttcgtctgca cgcgctgcgc
    20341 gtggacggcg gtgcggtggc gaacaatttc ctgatgcagt tccagtccga tattctcggt
    20401 acgcgcgttg agcgcccgga agtgcgcgaa gtcaccgctt tgggtgcggc ctatcttgct
    20461 ggtctggcgg ttggcttctg gcagaatctt gacgagctgc aagagaaagc ggtgattgaa
    20521 cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa
    20581 aaagcggtca aacgcgcgat ggcatgggaa gaacacgacg agtaataatg attgcctgat
    20641 gcgacgcttg cgcgtcttat caggcctacg agtcggatac acatccggca tcacgcagac
    20701 tctgaaaccg tgcgggggca accccgcaca catcaataat cccgcccttc ccctgtgcta
    20761 cacttcgcgc cattccttac tgcttagagt ttgctatgag acgagaactt gccatcgaat
    20821 tttcccgcgt caccgaatca gcggcgctgg ctggctacaa atggttagga cgcggcgata
    20881 aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca
    20941 ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga agcaccgatg ctctacattg
    21001 gtgaaaaagt cggtactggt cgcggcgacg cggtagatat tgctgttgat ccgattgaag
    21061 gcacgcgcat gacggcgatg ggccaggcta acgcgctggc ggtgctggca gtaggcgata
    21121 aaggctgttt cctcaacgcg ccagatatgt atatggagaa gctgatcgtc ggaccgggag
    21181 ccaaaggcac cattgatctg aacctgccgc tggcggataa cctgcgcaat gttgcggcgg
    21241 cgctcggcaa accgttgagc gaactgacgg taacgattct ggcaaaaccg cgccacgatg
    21301 ccgttatcgc tgaaatgcaa caactcggcg tacgcgtatt tgctattccg gacggcgacg
    21361 ttgcagcctc aattctcacc tgtatgccgg acagtgaagt cgatgtgctg tacggcattg
    21421 gcggcgcgcc ggaaggcgtg gtttctgcgg cggtgatccg cgcattagat ggcgacatga
    21481 atggtcgtct gctggcacgt catgacgtca aaggcgacaa cgaagagaat cgtcgcattg
    21541 gcgagcagga gctggcacgc tgcaaagcga tgggcatcga agccggtaaa gtattgcgtt
    21601 tgggcgatat ggcgcgcagc gacaacgtca tcttctctgc caccggtatt accaaaggcg
    21661 atctgctgga aggcattagc cgcaaaggca atatcgccac caccgaaacg ctgctgatcc
    21721 gtggtaaatc acgcaccatt cgtcgcattc agtccatcca ctatctggat cgtaaagacc
    21781 cggaaatgca ggtgcacatt ctctgagtga tttgatcgat tgagccttcc agtccttcgg
    21841 gactggaatt tttttgttcg gagaacgaag ataaggcaag tcaatcaaaa caggagaaaa
    21901 acatggctga ttgggtaaca ggcaaagtca ctaaagtgca gaactggacc gacgccctgt
    21961 ttagtctcac cgttcacgct cccgtgcttc cgtttaccgc cgggcaattc accaaacttg
    22021 gccttgaaat tgaaggcgaa cgcgttcagc gtgcctactc ttacgttaac tcgcccgaca
    22081 atcccgatct ggagttttac ctggtcaccg tccccgatgg caaattaagc ccgcgactgg
    22141 cggcgctaaa accgggtgat gaagtgcaag tggttagtga ggcggcaggc ttctttgtgc
    22201 tggatgaagt ccctgactgc gaaacactgt ggatgctggc gactggtacg gcgattggcc
    22261 cttatttatc gattctgcaa ttgggcaaag atttagaacg cttcaaaaac atggtgctgg
    22321 ttcacgccgc acgatatgcc gccgacttaa gctatctacc tctgatgcag gaactggaaa
    22381 aacgctacga ggggaaactg cgcattcaga cggtggtcag tcgggaaacg gctgcgggtt
    22441 cgctcactgg gcggataccg gcgttaattg aaagtggtga actggaaagc gcggttggcc
    22501 tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg caatccacag atggtgcgcg
    22561 atacacaaca gttgctgaaa gagacccggc agatgacgaa acatttacgt cgccgaccgg
    22621 gccatatgac agcggagcat tactggtaag cggttacttg tcgataaacg gcacgatcaa
    22681 gagatccgca ctcaacttat tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa
    22741 tgagtggtga tgaccacaga caaggaggtc gcactgctct ttttgcataa tttccagcag
    22801 tgtttccggc atttctccgc gttcaatacg cagttttgtc ttcggccatt gaatattttt
    22861 cgtcagttta tacagcttgt tatccgactt attcttcaac aattgaagaa tatcttctgt
    22921 tgcagggaag tagatacccg gatacaactc gcttaagcca tcatcaatat gaattaacgt
    22981 caggtgagcg tcattatgtc tggcgagctc cagggcttta ttcaccagta aggcatcttc
    23041 ttcattcccg gaaattgcca cgccaatgtg tttataagcc atgtttaact ccttctaaag
    23101 caactccatc aagctaacga acgcagtgat aactcaaaaa taatcatctc ggcctggcat
    23161 gagaaggtga aggccgcatc aagctcaact tgcccttcca gatctttaat cttataggcc
    23221 acgctggccc cttcatctgc agaggatgca gcggcggcaa gctctttcag tttggtcata
    23281 aaggactctg cttcttcgcg ggtggcaaaa aagcgcgaaa acttactggt acagttgtcg
    23341 ttatcaatca ccgtaccgat atcaatcgca catcctttag tactgcattt atctacgaca
    23401 tctttcatag ggcacctctg tatattcgcc tctctgtcga ttacgactta agaataatgc
    23461 gccgttaaag gaagaacaaa caagtaatga tcttatggat taccattttt tttgatattg
    23521 cttaaggtga agagttcaga aggattttcc gggcattgtc acttgcatcg tggaaataac
    23581 aatcccggaa aaaaatagcg ataagagata tattaaaggt taattacttg atttattgtc
    23641 ggctttatac ttcacatcct gggtatcttt accatattta ttttcgcctt gtgtgccaac
    23701 aaacgcacca agatcgataa gcatcatcac cagaatcagt gttgggataa aacgccccac
    23761 cgcccattgc catacccccg gcaaaattgc ccagttaccc gccagcagca tccacgccac
    23821 aatcatcaga aatgcccatg cgccggaacg cccgcgatca tgcaagcgct taacggttac
    23881 tgccgctgtt ggccaaagca agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa
    23941 atttttaccc gccagtgaga aaagcaccag catgcctgcg aaccacaggc ctatccaaat
    24001 ccagaaatca cggcgtccaa tacgcccttt aaatgagaat aaccattgct gtatggtcat
    24061 gtaagttcct tgatggttgt cttttccagg attctaccct tttgacaagg gcgacagata
    24121 tcgttttaat cggagccagt cataacaaaa ggtactgtca atgaagccag ggtgtacgct
    24181 gtttattctc ttatgttctg cattaaccgt tacaacaacg gcgcatgcac aaacaccaga
    24241 tacggcaacg accgcgcctt atctgctggc tggagcccct actttcgatc tctccatcag
    24301 ccagtttcga gaagacttta acagccagaa tcccagcctg ccactgaacg aatttcgtgc
    24361 catcgacagc agtcccgaca aagccaatct cactcgtgca gcaagtaaaa ttaacgagaa
    24421 cttgtatgct tctactgcgc tggagcgcgg taccttaaaa atcaaaagca ttcaaataac
    24481 ctggctaccc atccaggggc cagagcaaaa agccgcgaaa gcgaaagctc aggcatacat
    24541 ggcagcggtg atccgcacac tcaccccatt aatgaccaaa actcaaagcc agaaaaaact
    24601 gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac accgagacag aaggtgcact
    24661 gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa
    24721 gctggcgcta tctgaatcgc ttgaaggttt gaataaatga caaaaagcaa agcctttgtg
    24781 ccgatgaatc tctatactgt ttcacagacc tgctgccctg cggggcggcc atcttccttt
    24841 attcgcttat aagcgtggag aattaaaatg cgacatcctt tagtgatggg taactggaaa
    24901 ctgaacggca gccgccacat ggttcacgag ctggtttcta acctgcgtaa agagctggca
    24961 ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag
    25021 cgtgaagctg aaggcagcca catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc
    25081 ggcgcattca ccggtgaaac ctctgctgct atgctgaaag acatcggcgc acagtacatc
    25141 atcatcggcc actctgaacg tcgtacttac cacaaagaat ctgacgaact gatcgcgaaa
    25201 aaattcgcgg tgctgaaaga gcagggtcta actccggttc tgtgcatcgg tgaaaccgaa
    25261 gcggaaaacg aagcgggcaa aactgaagaa gtttgtgcac gtcagatcga cgcggtactg
    25321 aaaacccagg gtgctgcggc attcgaaggt gcggttatcg cttacgaacc tgtatgggca
    25381 atcggtactg gcaaatctgc aactccggct caggcacagg ctgttcacaa attcatccgt
    25441 gaccacattg ctaaagttga cgctaacatc gctgaacaag tgatcattca gtacggcggc
    25501 tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc cagacatcga cggcgcgctg
    25561 gttggcggtg cttctctgaa agctgacgct ttcgccgtga tcgttaaagc tgcagaagcg
    25621 gctaaacagg cttaagtctg acaggtgccg gatttcatat ccggcactta ctttcctaaa
    25681 ctcctcgcct taacgcaaaa tctcacactg atgatcctgg atttcttcgg ctgaagcacg
    25741 gttcagcgtc agtagatttc gttgtgtcgc cagcaataca aatgagttat cactctgccg
    25801 taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc tcaggcactt cttccgccag
    25861 catcataaat gggctgcgtt gtaccagttc gctttccgtt acccgacgcg ccaggtactc
    25921 atgcccacgc aaaccacctg gcagtggcaa ccagcggcta ctgatgttcg ccagattgtt
    25981 atccagctgt tcgcgcacat caggacgaat acaagaaata tgaatatgaa aatggttttg
    26041 cgtacgcccg gtgcgggagt tgatcgccag agataccgcg cgatcgggaa caggctggcc
    26101 gtatttttgg ctcataaaat cacgcgcctg ccaggctaac cagaaaaaat taggcgttga
    26161 cggatcggtc aacaatgggc tttcagtacc gttaatacgg tacgttggca ttaacagata
    26221 ttgcagtggg ccatgacgat cttttaaaac cacgtatccg gcgttgggtt tgacttccgc
    26281 acatggcgac gggttttggt tttcctgctg gttgggcaaa cattgctcaa gaactatctt
    26341 acgtaacgtg tctgactctt caccggttaa tttccagtaa ccaataccgg cagtcacaac
    26401 ggcgataact atcatcacca aaaaaagaag acccgctttt ttcatctttt tttccctgta
    26461 cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat aaaaaaccca gtggcgagaa
    26521 accaccgggc ttgataatta tcctggggaa atcagcgttt gctgatctga tcgaacgtac
    26581 cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg
    26641 tgaataactt caattttgga aacgcatttt cgtacttttt cgctacctca gcgtcgcgcg
    26701 gacggtagta gtttttcgct gcaatttcct gaccttctgg cgagtagaga tatttcaggt
    26761 aggcttccgc cacctctttg gtgccttttt tctcgaccac tttatcgacc accgacacgg
    26821 ttggctctgc gaggatagac tcactcggcg tgacgatttc gaatttatct ttccccagtt
    26881 cattcgctgc cagcagagct tcgttttccc aggcaatcag tacatcgcca attccgcgct
    26941 cgacaaaagt gttagtggat ccacgcgcgc cagaatccag aacttcgacg tttttataca
    27001 gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt gttgtgatga agcgcgtagc
    27061 cccaggctgc caggtaattc cagcgcgcgc caccagagct tttcggatta ggcgtgatca
    27121 ccgaaacacc cggtttaatc agatcgttcc agtcatggat ctgcttcgga ttacccttac
    27181 gtaccaggaa aacaatggtg gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc
    27241 actctttatc aatccgcccg cgttccgcaa ttgcgtccac gtcataggcc agagccagcg
    27301 tgacaacatc agcttcaata ccgttgatta ccgacgtcgc ttgtttgcct gaaccaccgt
    27361 gtgactgacg aatcaccacg ttatcgccag tttgctgttt ccagtgggcg ctgaatgcct
    27421 tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga aacgttaaga agctgaatat
    27481 cctttgccat aacgctggtt gccaccagca aaaatgttaa ccctacgccc cactttttca
    27541 tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg tgccagaagg ttgaccaaac
    27601 attaaagaat aaaaaaagat tggctataac ttgcgagtat atgttggggg attaaaaagg
    27661 cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt gtccatgccg gatgcggcac
    27721 aaacgcctta tccggcctac aaaagtttgc aaattcaata gattacagaa ttcatgtagg
    27781 cctgataagc gaagcgcatc aggccttttt gcttctgtca tcggtttcag gctaaaggaa
    27841 tctgcctttt tccgaaatca ttaatacagt tttttcgcgc agtccagcca gtcgcctttg
    27901 aacggacgct tcatgttttc gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc
    27961 tggataccta cgcaacgacc accgtaacct gccagcagca gatcgatagc gtaagcgccc
    28021 atacgggaag ccagaatacg gtcgtaaggc accggagaac caccgcgctg gatgtggccc
    28081 agcacagttg cgcgggtttc acgaccggtt tctttctcga tgaaatgcgc cagttcgtca
    28141 acatcacaca tatgttcggt aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc
    28201 gctttgattt cgtttaccag gtcttcacgg ctgaattcaa cttccggaac cacaacgaat
    28261 tcacagccac cggcaatggc cgcagccaac gtcagatcgc cacaataacg gcccatcact
    28321 tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct
    28381 acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg
    28441 tcgatagtgc ccggcaggcc gatgcacggg aagcccattt cggtcagacg cattgcaccc
    28501 atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga tcccacgttt tttcaggttt
    28561 tcgatagcca cggcgcggat gttctcgtcg cggaattccg ggaaacgcgc agaaccgagg
    28621 aacgtaccgc cacggttgat catgtcagaa acgctgtaac ggtctagctg taccatacgg
    28681 tcttcataca gacccagata gccgtcataa atgcccatta cttccagacc ttctgtcagc
    28741 gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc
    28801 aacacaccga ttttcttaat catgactacc tctgaacttt ggaatgcaaa atgaaatctg
    28861 ttgccggaag tcttcttgca catcgaagtg atccaacgaa tgtgcaaata gtataacaat
    28921 cacttcctgc tgaattgatt caggtcaggc caaatggcgg tattttatac acaaaatgcg
    28981 ggtctggctc tcttttatac tgattatgaa agcatagacc gtttaccctc cctgggtacg
    29041 acggaacagg ggtcctgatg gataatcaca tccgatcccg gaaaacgccg taaaatagcc
    29101 tgctctacct gatccgccac catatgtgcc tgaaccaaag gcagagagtc ttccatttcc
    29161 aaatgaatct gaataaagcg ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg
    29221 ctaacacccg gccaggaagt cacgatatca ataatttctt gccgttcctc atcaggcaat
    29281 gcgcgatcca gtaatgactg taccgcctca tatcccatgc gtaacgcgct atataaaata
    29341 tagatgccga tccccaatgc aaacagagca tcggcgcgat gccagccgta ccaggacaac
    29401 cccagcgcca gcagaattgc gccgttcatc ataacatcag actggtaatg tagcatatca
    29461 gcccgcaccg cctggctttg cgtccggcgc accacccaac gctgaaacga gacaaggata
    29521 atcgtacaaa ttagcgccac aattgtcacg ataaccccga cgcctggatc tgtcatcggt
    29581 gttggagata ccagatgttg aatacccgtc aaaaacagga atagtgccga accggagata
    29641 aacatacttt gcgccagcgc cgcgagggac tcagctttac cgtgaccaaa cgagtgatta
    29701 tcgtcggcag gttgcaggga atatcgcacc accagtaaat tcgtcaacga cgcaccgata
    29761 tccaccagcg aatccaccag cgcggcgaga atactcaccg acccggtata ccaccatgca
    29821 aaaattttaa tcagcaatag cagcgaagcc atcgccgtcg cagcaatcgc cgcccgactg
    29881 accagccgtc cataagattg attcataaat actcccgcta tcaactgacg gtagtataac
    29941 ggaagcaaat catctgcaat gcattaagca gcaggcaaat tgaggataaa aaaaaccccc
    30001 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    30061 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    30121 gtcgcgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    30181 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    30241 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    30301 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    30361 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    30421 catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga
    30481 accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc
    30541 ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt
    30601 ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta
    30661 gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt
    30721 tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct
    30781 tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg
    30841 atttactttt gcttgatgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac
    30901 ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc
    30961 gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg
    31021 agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgaa caacagcaaa
    31081 acaacgacaa cggttcaccg acactggaag ttgatgcctt aagtctcaat cctggtcgcc
    31141 aggaagccac cttcgacggg caaacgctgg agttaaccgg tacggagttt accctgctct
    31201 atttgctggc acagcatctg ggtcaggtgg tttcccgtga gcatttaagc caggaagtgc
    31261 tgggcaagcg cctgacgcct ttcgaccgcg ctatcgatat gcacatttcc aacctgcgtc
    31321 gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct
    31381 atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg
    31441 gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca
    31501 gatgaccgag cttctggata gcgaacagcg tcagggtctg atgattgagc agcatgtcga
    31561 agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc
    31621 gattgataag tgggcaccgc caggacagcg tttgttactg gtgaccaccg aaggccgcgt
    31681 gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa
    31741 cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt
    31801 gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc
    31861 cgatttcata aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgctggt
    31921 cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa
    31981 aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg
    32041 gccacaggaa ttccttgccg ccggtgccag ttttaaccag atggtcaccg cgctggagcg
    32101 catgatgacc tctcagcagc gtctactttc tgatatctct cacgaactgc gcaccccgct
    32161 gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa gcaaggaact
    32221 ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc tgttggtgat
    32281 gtcacgtaat cagcagaaaa atgcgctggt tagcgagacc atcaaagcca accagttgtg
    32341 gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt cgttgacggt
    32401 taacttccca cctgggccgt ggccgctgta cggcaacccg aacgccctgg aaagtgcgct
    32461 ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag tgggctttgc
    32521 ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga
    32581 agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg atcgtgaatc
    32641 tggcggtaca ggtttggggc tggcgattgt tgaaaccgcc attcagcagc atcgtggctg
    32701 ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta
    32761 taagcggtca taacgtcaac cttcaggtaa aaaaatgcct gatgcactac gcttatcagg
    32821 cctacaaaac ctgttgaatt tatgggtttt gtaggctgga taaggcgttt acgccgcatc
    32881 cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg
    32941 gggttatttt ccccacaaac ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt
    33001 ttgcatcgtt cgcgtccagc ttttcgataa ccctgcaacg gagagtaaac ggtgatactg
    33061 atcgtcatca aacggcatat gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct
    33121 gacacgggaa accaactcca gtggcgcatc agcagatacc aaccctggtg caattacgct
    33181 atacaaccac cccactttac ccgtgttttg catcaactgc gctatatcgc tgatatcaaa
    33241 atggtaattg agtttatagc aaggtgagcg tggctggctg acctgaatta atgcctctcc
    33301 ccagcggaaa atatcaccaa tataaacatt actttccgtc agaccgtcgg ttgagagatt
    33361 ttcgccaaac gcaggcgcaa caaacagctc cgcctgttcc ggaaattccc gcgcccagta
    33421 gagataatgc tcacgaggat aatgacacag cgctctgtct ggcccgccgt ggatcttttt
    33481 ctccgcctgt tcgtcacctt ccagccccag gtccgtcagc atcaactcac catcaacctg
    33541 gattttggca attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac
    33601 atcaaccgga tatcgcatct gttccctctc cacgagaaat ttccccatta gaccatattt
    33661 attttatgtc aggcgtgctg ttttcgcggt aagtatccag gcagaaacgc aattgaaaac
    33721 cggacgaacg ttagttcatc cggctgtggg gattaaggaa gctggtttat ttcaccgtac
    33781 ctaaaatttc gattttcgcc gctctggctt tgactttacg tgaccagata gaggtaagaa
    33841 ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac cagcgaagtt gctgccggag
    33901 ccataggttt aaacgcaggc accatttctg caatcagcac aggtgtcgct accgcggccc
    33961 cagcggaact ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg
    34021 cgataatcaa cggaataccg gtcacgataa ttactgccac acccaacagg atccccagca
    34081 aacctgtctg ggcaattaca gtcaaatcaa tggtattgcc cagtgcgaag gcaaagaatg
    34141 gaatcagcgt ctgcaccgct ttgctgaaaa attctcgtaa ctcagggtca aggttcccca
    34201 gggcaaagcc caccaggaac ggcaatactg cgccgacgaa aacatgcggt tcaaacgagg
    34261 caatcccggc agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca
    34321 acacaaatgc cccagcttct tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc
    34381 cgccgttggt catatccatc gccgccacca gcgccaacgt tgaaagtccg gcaaaaaatc
    34441 caacttcaac accatgttcc ggaataatgc gcgaggcaat tgccgcaacc acccacgcga
    34501 cggcaatttt agttaccacc agcgtaccgg atttacgcag taccgttccc gtcgcgctta
    34561 attttattga cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa
    34621 tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca
    34681 gtgcgccaag gaatagcggg acgagcatca tcccccccgg gattttctca atcgagcgtt
    34741 ttatctgcat ttccatcacc tgtcggcata tttttaaggg ctgatgcgtc attcctcaac
    34801 ggagcatcag tgttgatgga aacgaagata aacattcatt tgatcaataa aagtgatcaa
    34861 aaccacatca attgaaacga tgttttattt ttatcggatc gttgttttta ttttgaaagt
    34921 gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt ttaagctgca atgcggcgta
    34981 aacgcctcat tgcagcaggc gtcaaatgat tattttttcg ccgcgaaacg tgccgctgct
    35041 tcgtcccagt tcaccacgtt ccagaactct ttaatgtagt ccggacggcg gttctggaat
    35101 ttcaggtagt aagcatgttc ccacacatcc aggcccacaa tcgggaagcc ggaagcgcca
    35161 gaaatggctt cgcccatcag cggagagtcc tggttagcag tagaaaccac cgccagttta
    35221 tcgccttttt gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttttca
    35281 aattctgctt tgaaattatc aacggaaccg aaatcacgtt cgatagccgc tttcaggtcg
    35341 ccctgcaggg tggtgccttt tttcagacct ttccagaaca gactgtggtt agcgtgaccg
    35401 ccagcgttat tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc
    35461 agctcttcaa ccggcaggtt agcgaattct ggcaggcttt ccagcgccgc attggcgttg
    35521 ttaacgtagg cctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc
    35581 ggttccaggg catcgtaagc atacggcagg gatggcaggg tataactcat attcatctcc
    35641 agtgtggtcg ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt
    35701 aaatgatatt gaaaatgatt atcaatgccg tacttttcat aagggtatgg ttttgcagga
    35761 aaatgcccaa gatgtgaagc aaatcaccca cttaatgccg tgattgccag taaatcggca
    35821 gcggcggcaa caggcgaaag gttaatcgac agctcaattt ttacactcat ctcgtcggag
    35881 atgtgacgcg acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat
    35941 attttggcat ttgatcggcg cggccagtgc agcctgtttt tacgcgccgt tcaaaaaagt
    36001 aaaaaagtgg tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattatcct
    36061 gccgtgggcc atcagcgccc tgttactacc gaatttctgg gcgtattaca gctcatttag
    36121 tctctctacg ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa
    36181 ctacggcctg accatgcgtt atctcggcat gtcgatggga attggcatcg ccattggcat
    36241 tacgttgatt gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgctgat
    36301 taataccgaa ggcggacgca tgacgttgct cggcgttctg gtggcgctga ttggcgtagg
    36361 gattgtaact cgcgccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt
    36421 caatctgaaa aaagggctgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc
    36481 ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct gccgcgctgg gcgtcgatcc
    36541 cctgtatgtc gctttgccaa gctatgttgt catcatgggc ggcggcgcaa tcatcaacct
    36601 cggcttctgc ttcattcgtc tggccaaagt gaaagatttg tcgctaaaag ccgacttctc
    36661 gctggcaaaa ccgctgatca ttcacaatgt gttactctca gcactgggcg ggttgatgtg
    36721 gtatctgcaa ttctttttct atgcctgggg ccacgcccgc attccggcgc agtatgacta
    36781 catcagctgg atgctgcata tgagcttcta tgtattgtgc ggcggtatcg tcgggctggt
    36841 gctgaaagag tggaacaatg caggacgccg tccggtaacg gtgttgagcc tcggttgtgt
    36901 ggtgattatt gtcgccgcta acatcgtcgg catcggcatg gcgaattaat ctttctgcga
    36961 attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa
    37021 atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg
    37081 ctggagaaga tattgcgcat gacacactct gacctgccgc agatattgat tgatggtcat
    37141 tccagtctgc tggcgaaatt gctgacgcaa aacacgctca ctgcatgatg cctcatcaca
    37201 aaatttatcc agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc
    37261 cagcaatgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag
    37321 caacatcacc aactgcccga acagcaactc agccatttcg ttagcaaacg acacatgctg
    37381 actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc ccacgctacc
    37441 taagcgccag tgtggttgcc ctgcgctggc gctaaatccc ggaatcgcgc cctgccagtc
    37501 aagattcagc ttcagccgct ccgggcaata aataatattc tgcaaaacca gatcgttaac
    37561 ggaagcgtag gagtgtttat cgtcagcatg aatataaaag agatcgccac gggtaatgcg
    37621 ataagggcga tcgttgagta catgcagacc attaccgcgc cagacaatca ccagctcaca
    37681 aaaatcatgt gtatgttcgg caaagacatc ttgcggataa cggtcagcca cagcgactgc
    37741 ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc
    37801 tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag
    37861 attcgcgtta ttgcagaaaa ccatcccgtc cctgacgaat atcacgcggt gaccagttaa
    37921 actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcag cgataggcga
    37981 tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc
    38041 ggttcaggta acgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac
    38101 gcaatgaaag agaaaattga gccgccactg catcccaatt cacctcatcg gcaaaatggt
    38161 cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccacg ctctcctgca
    38221 aactgctttt acgcagtaag agcagtaatt gcataaacag aatttcacga ctggcggtcg
    38281 agggtaaatc atttccctct tcttgttgtt ccaactgtgc aaccagttgg cgcacctgct
    38341 gtaatacgca atggttaacg cgccagtgag acggatactg cccatcctgc tcttgtggca
    38401 gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg
    38461 tcagacacag attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaaa
    38521 ccgtcccacc ggtgatggta tagggctgcc cattaaatac atgaataccc gtgccatgtt
    38581 cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc
    38641 ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg
    38701 tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt
    38761 accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga
    38821 attttcagga aatgcggtga gcatcacatc accacaatga agcaaattgt gaacatcatc
    38881 acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcacg
    38941 aattcaggcg ctttttagac tggtcgtaat gaaattcagc aggatcacat tatgaccttt
    39001 cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac
    39061 gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc gttttaacaa tggcctgcat
    39121 agccagaacg gttatgtcac ctggaatgtg gatagcctgg aaagtgccat tcgccttgga
    39181 ttaaacaagg tgtgcgagga agggattcgt atcgatagca ttgggattga tacctggggc
    39241 gtggactttg tgctgctcga ccagcacggt cagcgcgtgg gtctgcccgt tgcttaccgc
    39301 gatagccgca ccaatggcct aatggcgcag gcacaacagc aactcggcaa acgcgatatt
    39361 tatcaacgta gcggcatcca gtttttgccc ttcaatacga tctatcagct acgtgcactg
    39421 acagagcagc aaccagaact tattccacac attgctcacg ctttgctgat accggattac
    39481 tttagttatc gcctgaccgg caagatgaac tgggaatata ccaacgccac gaccacgcaa
    39541 ctggtcaata tcaatagcga cgactgggac gagtcgctac tggcgtggag cggtgccaac
    39601 aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca taggtcactg gatttgcccg
    39661 cagggtaatg agattccggt ggtcgccgtt gccagccatg ataccgccag cgcggttatc
    39721 gcctcgccgt taaacggttc acgcgccgct tatctctctt ctggcacctg gtcattgatg
    39781 ggcttcgaaa gccagacgcc atttaccaat gacacggcgc tggcagccaa tatcaccaac
    39841 gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt atggctgctt
    39901 cagcgagtgc ttcaggagcg gcaaatcaac gatcttccgg cgcttatcgc cgcaacacag
    39961 gcacttccgg cctgccgctt catcatcaat cccaatgacg atcgctttat taatcctgac
    40021 gagatgtgca gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt
    40081 gatgctgaac tggcgcgctg tattttcgac agtctggcgc tgctgtatgc cgatgtgttg
    40141 catgagctgg cgcagctgcg tggtgaagat ttctcgcaac tgcatattgt cggcggcggc
    40201 tgccagaaca cgctgctcaa ccagctgtgt gccgatgcct gcggtattcg ggtgatcgcc
    40261 gggcctgttg aagcctcgac gctcggcaat atcggcatcc agttaatgac gctggatgaa
    40321 ctcaacaatg tggatgattt ccgtcaggtc gtcagcacta ccgcgaatct gaccaccttt
    40381 acccctaatc ctgacagtga aattgcccac tatgtggcgc agattcactc tacacgacag
    40441 acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctt gcgaaacagc
    40501 gtttcgcggc agtagggatt gatgtcgagg aggcactgcg ccaacttgat cgtttacccg
    40561 tttcaatgca ctgctggcag ggcgatgatg tttccggttt tgaaaacccg gaaggttcgc
    40621 tgaccggtgg tattcaggct actggtaatt atccgggcaa agcgcgtaat gccagcgagc
    40681 tgcgtgccga tctggaacag gctatgcggc tgattccggg gccaaaacgg cttaatttgc
    40741 atgccattta tctggagtca gacacgccag tctcacgtga ccaaatcaaa ccagagcact
    40801 tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg tctggatttt aacccctcct
    40861 gtttttcaca tccgctaagc gccgatggct ttacactttc ccatgccgac gaccgcattc
    40921 gccagttctg gattgaccac tgtaaagcca gccgccgtgt ttcagcctat tttggtgagc
    40981 aactcggcac accgtcggtg atgaacatct ggatcccgga tggcatgaaa gatatcaccg
    41041 ttgaccgtct cgccccgcgt cagcgtttgc tggcagcact ggatgaagtg atcagcgaga
    41101 agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc attggcgcgg
    41161 agagctacac ggttggctcc aatgagtttt acctggggta tgccaccagc cgccagacgg
    41221 cgctgtgcct ggacgccggg catttccacc cgactgaagt gatttccgac aagatttccg
    41281 ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca
    41341 gcgatcacgt agtgctgctg gatgatgaaa cccaggcgat tgccagtgag attgttcgtc
    41401 acgatctgct cgaccgggtg catatcggcc tcgacttctt cgatgcctct atcaaccgca
    41461 ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg
    41521 aacctaccgc tgagctacgc aagctggaag cggcgggcga ttacactgcg cgtctggcgc
    41581 tactggaaga gcagaaatcg ttgccgtggc aggcggtatg ggaaatgtat tgtcagcgtc
    41641 acgatacgcc agcgggtagc gaatggctgg agaacgtgcg gacttatgag aaaaaaattt
    41701 tgagtcgccg cgggtaaaca ctgccggatg cggcacaagt gccttatccg gcctacgggt
    41761 cggcaatagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga
    41821 tgcggcacga gtgccttatc cggcctacgg gtcagcaaca gttgtaggcc tgataagacg
    41881 cgacagcgtc gcatcaggca ttgattgccg gatgtggcgc gagtgcctta tccggcctac
    41941 aggtcgtgca tccgacaaca ccgaattaac aggaacacag aacatgcaaa acataaccca
    42001 gtcctggttt gtccagggaa tgatcaaagc caccaccgat gcctggctga aaggctggga
    42061 cgagcgcaac ggcggcaacc tgacgctgcg cctggatgac gccgatatcg caccatatca
    42121 cgacaatttt caccaacaac cgcgctatat cccgctcagc cagcccatgc ctttactggc
    42181 aaatacaccg tttattgtca ccggctccgg caaattcttc cgtaacgtcc agcttgatcc
    42241 tgcggctaac ttaggcatcg taaaagtcga cagcgacggc gcgggctacc acattctttg
    42301 ggggttaacc aacgaagccg ttcccacttc tgaacttccg gctcactttc tctcccactg
    42361 cgagcgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc acgccaccaa
    42421 cctgatcgcc ctcacctatg tacttgaaaa cgacaccgcg gtcttcactc gccaactgtg
    42481 ggaaggcagc accgagtgtc tggtggtatt cccggatggc gttggcattc tgccgtggat
    42541 ggtgcccggc acggacgaaa tcggccaggc aactgcacaa gagatgcaaa aacattcgct
    42601 ggtgttgtgg cccttccacg gtgtgttcgg cagcgggccg acgctggatg aaaccttcgg
    42661 tttaatcgac accgcagaaa aatcagcaca agtattagtg aaggtttatt cgatgggcgg
    42721 catgaaacag accatcagcc gtgaagagtt gatagcgctc ggccagcgtt tcggcgttac
    42781 accactcgcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag cctttgtcat
    42841 gcaggtaaac cccgacgccc acgaagagta tcagcgtcgg cataatccca tctggccgga
    42901 actggaagca gtgctgaaat ctcacggtgc gcataactac gccatctatc tcgacaaagc
    42961 gcgtaatctg ctatttgcca cgatagagat tgaatctgaa gagcggtgga atgcggttgc
    43021 cagtacagat gtttgccaac gttggtggaa atatatgacc gacgttatgc ccgctaatgc
    43081 ggataacagc ccagtcagta gcgaactgca agaagtgttt tacctgcctt aatgagttgc
    43141 ggtgccggaa aatatccagc accgcccttt catcagaaga agtatttaaa gcgaacgcgt
    43201 gcgccgtaac ggtcatcact atcgtcgccg tgtatcttat cgccgtcgct atccagaatg
    43261 gagtaatacg tgccgaggta aatattaaag ttctccatat ccatcacatt agcgaactga
    43321 taagacgcat gaatggtgtg aatgttgtat gttccttcat catcaatcca gcaatcgtta
    43381 tcacaaacca cgccactaaa ttcatcaatc ttattatgtg cgtagatata acccagctcg
    43441 aaacgtttcc acagcgcgtt aatcccagcg gtgaaatctt tctcattatt agcatctaaa
    43501 taggcggtat taagattaac cacgatgcca ttttccggat cggttttcag gccattccag
    43561 gtcatgctca tgccataacc ggtacgatcg gactgatcga caaaattacc cttgctatcg
    43621 gtataaccat aagcattatt taccacgttc gcttccatcg ctgcggaaac ggtgaattct
    43681 tccgtcggcg accaggcaat taccggacgc agataagcaa catttttctg ctgttccata
    43741 tcgcgtccat gataattacc gtcgttatat aaagatgttc cgtcttccag taacgtgttt
    43801 aattcaaaat accagttatc gagttgtttg ctgacgagga aattaccgcc agcgttagaa
    43861 cgcccgcggc cctctttcat catatagata taaccgctgc catcgtcata aagatcgttc
    43921 gcagtattac cggaatgttc aacaaaggta tcctgattca gcgggaacat atcgtaggct
    43981 tcaaaacggc ctactttgac cttccagtcg ttttctttgc caaagaagaa aaccgcatca
    44041 tcgatattta ctgaaccgtg catatccccc agcggttgtg cggagaaccc agcgaaatag
    44101 ccattatcca gttttcgcat accatcaaaa cctaacagaa tacgaccatt gagattccac
    44161 tgttcattag tgggatcgct attcacatca gcgttggtca ttgccagcag gccatgatta
    44221 ctttccgcat ccatattaaa ttctacgtca ccgtagattt ttaaatcgcc ataccctgaa
    44281 agcaggagat taggcggggt ggcgtttttc ttctctgact ccgtcgttga ttgaccgttt
    44341 accggcgtgt tgccctgtgc ggttttaatt tcgcggatct cggcggcttg ttgtgtctga
    44401 agctgtttaa cctgcccctc tgcttttgct gcccgcattt ctgccgcttc caggcgttct
    44461 tccagcaatg ccatgcgttg ttccagcgtt aaatgctgtg tttttgccat accggtacta
    44521 catgccaata ataaaccgat ggctaaagat aatgtcgatt ttttcattat ttacgttccc
    44581 tgaaataaag gagaccctac gctttccaaa aggaatacgt atatagagaa atataaggtg
    44641 tgcacttt
//
LOCUS       DACADA010000020        44072 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.020, whole genome shotgun sequence.
ACCESSION   DACADA010000020 DACADA010000000
VERSION     DACADA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.020"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            257..2962
                     /gene="malT"
                     /locus_tag="ISB17_001102"
     CDS             257..2962
                     /gene="malT"
                     /locus_tag="ISB17_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAP1979057.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3005..3487)
                     /locus_tag="ISB17_001103"
     CDS             complement(3005..3487)
                     /locus_tag="ISB17_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HAP1979058.1"
                     /translation="MMRPLVAKVAIRVTLAESSRKLPRHVAEREIATLAGSFSLHEQN
                     IHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAV
                     GEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE
                     "
     gene            3396..3791
                     /locus_tag="ISB17_001104"
     CDS             3396..3791
                     /locus_tag="ISB17_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1979059.1"
                     /translation="MSRSATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRY
                     NWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKA
                     SVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(3773..4531)
                     /locus_tag="ISB17_001105"
     CDS             complement(3773..4531)
                     /locus_tag="ISB17_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAP1979060.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(4548..5378)
                     /gene="glpG"
                     /locus_tag="ISB17_001106"
     CDS             complement(4548..5378)
                     /gene="glpG"
                     /locus_tag="ISB17_001106"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAP1979061.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(5423..5749)
                     /gene="glpE"
                     /locus_tag="ISB17_001107"
     CDS             complement(5423..5749)
                     /gene="glpE"
                     /locus_tag="ISB17_001107"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAP1979062.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            5939..7444
                     /gene="glpD"
                     /locus_tag="ISB17_001108"
     CDS             5939..7444
                     /gene="glpD"
                     /locus_tag="ISB17_001108"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP1979063.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(7498..8103)
                     /locus_tag="ISB17_001109"
     CDS             complement(7498..8103)
                     /locus_tag="ISB17_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979064.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(8106..8873)
                     /locus_tag="ISB17_001110"
     CDS             complement(8106..8873)
                     /locus_tag="ISB17_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979065.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(8976..10484)
                     /locus_tag="ISB17_001111"
     CDS             complement(8976..10484)
                     /locus_tag="ISB17_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1979066.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(10609..13056)
                     /gene="glgP"
                     /locus_tag="ISB17_001112"
     CDS             complement(10609..13056)
                     /gene="glgP"
                     /locus_tag="ISB17_001112"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAP1979067.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(13075..14508)
                     /gene="glgA"
                     /locus_tag="ISB17_001113"
     CDS             complement(13075..14508)
                     /gene="glgA"
                     /locus_tag="ISB17_001113"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAP1979068.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(14508..15803)
                     /gene="glgC"
                     /locus_tag="ISB17_001114"
     CDS             complement(14508..15803)
                     /gene="glgC"
                     /locus_tag="ISB17_001114"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAP1979069.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(15821..17794)
                     /gene="glgX"
                     /locus_tag="ISB17_001115"
     CDS             complement(15821..17794)
                     /gene="glgX"
                     /locus_tag="ISB17_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAP1979070.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(17791..19977)
                     /gene="glgB"
                     /locus_tag="ISB17_001116"
     CDS             complement(17791..19977)
                     /gene="glgB"
                     /locus_tag="ISB17_001116"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAP1979071.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(20250..21353)
                     /gene="asd"
                     /locus_tag="ISB17_001117"
     CDS             complement(20250..21353)
                     /gene="asd"
                     /locus_tag="ISB17_001117"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP1979072.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            21545..22138
                     /gene="yhgN"
                     /locus_tag="ISB17_001118"
     CDS             21545..22138
                     /gene="yhgN"
                     /locus_tag="ISB17_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAP1979073.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(22184..23479)
                     /locus_tag="ISB17_001119"
     CDS             complement(22184..23479)
                     /locus_tag="ISB17_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP1979074.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(23386..25482)
                     /locus_tag="ISB17_001120"
     CDS             complement(23386..25482)
                     /locus_tag="ISB17_001120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP1979075.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(25623..26963)
                     /gene="gntU"
                     /locus_tag="ISB17_001121"
     CDS             complement(25623..26963)
                     /gene="gntU"
                     /locus_tag="ISB17_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP1979076.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(26967..27494)
                     /gene="gntK"
                     /locus_tag="ISB17_001122"
     CDS             complement(26967..27494)
                     /gene="gntK"
                     /locus_tag="ISB17_001122"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP1979077.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(27633..28628)
                     /locus_tag="ISB17_001123"
     CDS             complement(27633..28628)
                     /locus_tag="ISB17_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HAP1979078.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(28721..29416)
                     /locus_tag="ISB17_001124"
     CDS             complement(28721..29416)
                     /locus_tag="ISB17_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP1979079.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(29539..30576)
                     /locus_tag="ISB17_001125"
     CDS             complement(29539..30576)
                     /locus_tag="ISB17_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1979080.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            30910..31398
                     /gene="yhhY"
                     /locus_tag="ISB17_001126"
     CDS             30910..31398
                     /gene="yhhY"
                     /locus_tag="ISB17_001126"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1979081.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(31459..33192)
                     /gene="ggt"
                     /locus_tag="ISB17_001127"
     CDS             complement(31459..33192)
                     /gene="ggt"
                     /locus_tag="ISB17_001127"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAP1979082.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            33312..33752
                     /locus_tag="ISB17_001128"
     CDS             33312..33752
                     /locus_tag="ISB17_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAP1979083.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(33739..34482)
                     /gene="ugpQ"
                     /locus_tag="ISB17_001129"
     CDS             complement(33739..34482)
                     /gene="ugpQ"
                     /locus_tag="ISB17_001129"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP1979084.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(34479..35549)
                     /locus_tag="ISB17_001130"
     CDS             complement(34479..35549)
                     /locus_tag="ISB17_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAP1979085.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(35551..36396)
                     /gene="ugpE"
                     /locus_tag="ISB17_001131"
     CDS             complement(35551..36396)
                     /gene="ugpE"
                     /locus_tag="ISB17_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAP1979086.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(36393..37280)
                     /gene="ugpA"
                     /locus_tag="ISB17_001132"
     CDS             complement(36393..37280)
                     /gene="ugpA"
                     /locus_tag="ISB17_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAP1979087.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(37378..38694)
                     /gene="ugpB"
                     /locus_tag="ISB17_001133"
     CDS             complement(37378..38694)
                     /gene="ugpB"
                     /locus_tag="ISB17_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAP1979088.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEARIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(39091..39804)
                     /gene="livF"
                     /locus_tag="ISB17_001134"
     CDS             complement(39091..39804)
                     /gene="livF"
                     /locus_tag="ISB17_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAP1979089.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(39806..40573)
                     /gene="livG"
                     /locus_tag="ISB17_001135"
     CDS             complement(39806..40573)
                     /gene="livG"
                     /locus_tag="ISB17_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAP1979090.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGMPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(40570..41847)
                     /locus_tag="ISB17_001136"
     CDS             complement(40570..41847)
                     /locus_tag="ISB17_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAP1979091.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(41844..42770)
                     /gene="livH"
                     /locus_tag="ISB17_001137"
     CDS             complement(41844..42770)
                     /gene="livH"
                     /locus_tag="ISB17_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAP1979092.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(42818..43927)
                     /gene="livk"
                     /locus_tag="ISB17_001138"
     CDS             complement(42818..43927)
                     /gene="livk"
                     /locus_tag="ISB17_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAP1979093.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT        10861 a        12057 c        10962 g        10192 t
ORIGIN      
        1 atgaagaaga aacccttgct taaatgaaac tgatgaacat aaggaaaata ccgtacaacc
       61 cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaacat ttggaattgt
      121 gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg
      181 gccgacctta taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttccac
      241 agtgaagtga ttaactatgc tgattccgtc aaaattaagt cgcccggttc gactcgacca
      301 taccgtggtt cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc
      361 gctgatcaca agtcctgcgg gctacggaaa gaccacgctc atttcccagt gggcggcagg
      421 caaaaacgat atcggctggt actcgctgga tgaaggtgat aaccagcaag agcgtttcgc
      481 cagctatctc attgccgccg tgcaacaggc aaccaacggt cactgcgcga tatgtgagac
      541 gatggcgcaa aaacggcaat atgccagcct gacgtcactc ttcgcccagc ttttcattga
      601 gctggcggaa tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa
      661 tcctgtgatc cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct
      721 tgtggtgttg tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgacca
      781 gctgctggaa attggcagtc agcaactggc atttacccat caggaagcga aacagttttt
      841 tgattgccgt ctgtcatcgc cgattgaagc tgcagaaagc agtcggattt gtgatgatgt
      901 ttccggttgg gcgacggcac tgcagctaat cgccctctcc gcccggcaga atactcactc
      961 agcccataag tcggcacgcc gtctggcggg aatcaatgcc agccatcttt cggattatct
     1021 ggtcgatgag gttttagata acgtcgatct cgcaacacgc cattttctgt tgaaaagcgc
     1081 cattttgcgc tcaatgaacg atgcactcat cacccgtgtg accggcgaag aaaacgggca
     1141 aatgcgcctc gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg
     1201 cgagtggttt tgctatcacc cactgtttgg taacttcctg cgccagcgct gccagtggga
     1261 actggcggcg gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg
     1321 atttcccagc gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga
     1381 tattctgctt aatcacgcct ggagtttgtt caaccatagc gaactgtcgc tgctggaaga
     1441 gtcgcttaag gccctgccgt gggacagttt gctggaaaat ccgcagttgg tgttattgca
     1501 ggcgtggctg atgcaaagcc agcatcgcta cggcgaagtt aacaccctgc tggcccgcgc
     1561 tgaacatgaa atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg
     1621 cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg gaacggctgg caaaactggc
     1681 actggaagag ctgccgccgg gctggttcta tagccgcatt gtggcaacct cggtgctggg
     1741 tgaagtgttg cactgcaaag gcgaattgac ccgctcactg gcgttaatgc agcaaaccga
     1801 acagatggca cgccagcacg atgtctggca ctacgctttg tggagtttaa tccagcaaag
     1861 tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg aaaaagcgtt
     1921 ccagctgatc aacgagcagc atctggaaca gctgccaatg catgagtttc tggtgcgcat
     1981 tcgtgcgcaa ctgttatggg cctgggcacg gctggatgaa gccgaagcgt cggcgcgcag
     2041 cgggattgaa gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt
     2101 gattcaatgc tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct
     2161 ggaaaacctg ctggggaatg gcaaatatca cagcgactgg atctctaacg ccaacaaagt
     2221 ccgggtgatt tactggcaaa tgaccagcga taaagccgcc gctgccaact ggttgcgtca
     2281 tacggctaaa ccagagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc
     2341 ccgtgcacaa atcttgctgg gcgagtttga atctgcggaa attgtcctcg aagaactcaa
     2401 tgaaaatgcc cgcagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgcttaa
     2461 tcaactgtac tggcaggccg gacgtaaaag tgacgctcag cgtgtgttgc tggacgcatt
     2521 aaaactggcg aatcgtaccg gatttatcag tcattttgtc atcgaaggcg aagcgatggc
     2581 gcaacaactg cgccagctta ttcaactcaa tacgctgccg gagctggaac agcatcgcgc
     2641 acagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga
     2701 gaatttcgtt gaacgtctgc taaatcatcc ggaagtgcca gagctgatcc gcaccagccc
     2761 gttgacccaa cgtgaatggc aggtcctggg gctgatttac tccggttaca gcaatgagca
     2821 aattgcaggc gagctggaag tggcggcaac caccatcaaa acgcatatcc gtaatctgta
     2881 ccaaaaactc ggtgtcgccc accgtcaggc cgcggtacaa cacgcgcaga aactgctgaa
     2941 gatgatggga tatggggtgt gagtttagcc ggataatgcg tcggatccgg cttacatctt
     3001 tacatcattc aatgctaacc cgcgttacgc catctgtttc tatcaaacca aaccgcaccg
     3061 gcaagaaacg ctccaccacg gcgatattgg tcagcagatg acatgaggga tgggcgaccg
     3121 taaattctcc cgcgcccgcc agcgccatcg gtagcaccag ttggtcggcg agatattccc
     3181 ccaccgccgc cgggcttgcc aggtagcgtt tcacctcttt caccaactgt gccgcgacca
     3241 cctcggcact gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata ttttcacttt
     3301 cgacttcaag cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct
     3361 gttcatgcaa ggaaaaacta cccgccagtg tagcgatttc acgctcagca acatggcgcg
     3421 gtaactttcg cgaactttct gccagcgtca cgcggatggc cacctttgcc actagcggac
     3481 gcatcacact ggatacagtg gaagatgaga tcaaccgtct gcgctataac tggcaggaga
     3541 gtcgcccctc cacgcttacg gcgttgctgg gcgcagaggc agaaaacatc gatctcttcg
     3601 accgtatgca actggaacac gttatcgcta tctgccgcca ggcaaagtcg ctttctgctg
     3661 ccggacgtga actgtttgac atttcgcgcc agggtaaagc cagcgtcaat gacgcggatc
     3721 ggctacgcaa atacctggcg cgttttggtc tgacatggga agcaatgcag gatcagcaca
     3781 gctccagttg aatatgatgg tccttcagca cctgcatcac gctcgcaggc ggcatcgtgt
     3841 cggtgtagac agcatcgacc atgctgatgc tacccatatt gaccattgcg ttacggccaa
     3901 attttgagtg gtcgacaacc agcataacgt ggcgcgagtt ctcaatgatg gcgcgtttgg
     3961 tgcgaacttc gtggtaatcg aactccagta gcgagccgtc gctatcgatg ccgcttatcc
     4021 ccagaatgcc gaaatcaagg cggaactggg agataaaatc gagcgttgct tcgccaatga
     4081 tcccgccatc gcggctgcgt aattcgccac cggcaagaat gatacggaaa tcctctttta
     4141 ccatcagcgt gttagcgacg ttgagattgt tggtcacaat gcgtaaattg ctgtgattaa
     4201 gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc gataaacagc gtcgagccat
     4261 tgggaatttg ctccgccact ttgcgggcga tgcgctcttt ttcttcggtc tgggtggcct
     4321 tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag cgccgcaccg ccatgatggc
     4381 gcaggatcag gttttgctct gccagctcgt tgagatcgcg gcgaatagtc tgcgggctaa
     4441 cggagaaatg ctctaccagc tcttcggtgc tgacataacc ctgctgttta accagttcga
     4501 taataccgtt gtgacgttgt gtttgtttca ttgataaatc cctggaatta ttttcgtttt
     4561 cgcgcattga gcgaatcaac aaaagccatc gctaaaccca cggctaaccc ggcgatatgt
     4621 gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc cggcgacaat ccagatcaac
     4681 gcaaagataa ttaacccacg ttgcaggtaa atgccacttt gcggatcgcg ttcgccacgt
     4741 agccagacgt agcccatcag cgcatacacc acgccagaaa gcccgccaaa ccacggcccg
     4801 ctgaattttt gctgcacata gccgcttaac agggcgctaa tgagagtaat gacaattagc
     4861 ttaccgctac cgaggcgttt ttccaccgca ccgccgagat accaccacca gagcaggtta
     4921 aagaggatat gcatcagcga gaagtgcatt aacgcgtggg tgaagtaacg ccagaactca
     4981 aatttcagcg tcggatcgaa tggccaggcc agccataaca tcacttcctg atcgccgaga
     5041 atttgcatgg cgataaacac caccacgcag gcgatcatca tcacccaggt taccggacct
     5101 gcgcgttcac gcaaggcggc aaagaaagga taacggcgat aatgcaggcc actgtcggta
     5161 tgaccggact gccaactggc ggcgagataa cgtggatctg ccgggttttc gagaaaacga
     5221 gccagctccg cccgcacgcg ttcggcctgg gactcatccg ccagccagac atcgctttgg
     5281 ttatgttgtt gaatcgtgag gataacaccc tgtgtcgcca tgtaatcaac aaacgcctgc
     5341 gccactcggg ggttagcaaa agaggtaatc atcaacatcg ttgctgtcgc ttattccaca
     5401 caaaagggga cagtataaag cgttacgcgc cgtacgccac ctctgcggga aactggcgct
     5461 gccaggcttc aaaaccgcca tcaatgctgt agaccacatc gtagccctgt tgcagcagat
     5521 actgcgccgc gcctttgctg ctattgccgt gataacacat caccatcacc ggtgtgtcaa
     5581 agtcgttatc acgcataaac gcgcccagtg tgtcgttagt taaatggaaa gcctgggtcg
     5641 catgccccat tgcgaaactc tgtggatcgc gaatatcgac cagcaccgcc tctttttcct
     5701 gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa ctgatccatg cgtctctctt
     5761 tctttacaaa caagtgggca aatttaccgc acagtttacg tcgaagcggc agataaacgc
     5821 cataatgtta tacatatcac tctaaaatgt tttttcaatg ttacccaaag cgcgattctt
     5881 tgctaatatg ttcgataacg aacatttatg aactttaacg aaagtgaatg agggcagcat
     5941 ggaaaccaaa gatctgattg tgataggtgg cggcatcaat ggtgctggta tcgcggcaga
     6001 cgccgctgga cgcggtttat ccgtgctgat gctggaggcg caggatctcg cttgcgcgac
     6061 ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc taccttgagc actatgaatt
     6121 ccgactggtc agcgaggcgc tggctgaacg tgaagtgcta ctgaaaatgg ccccgcatat
     6181 cgccttcccg atgcgttttc gcctgccgca tcgtccgcat ctacgcccgg cgtggatgat
     6241 tcgcattggt ctgtttatgt acgatcatct gggcaagcgc accagcctgc cgggatcaac
     6301 cggtttgcgt tttggcgcaa actcagtgtt aaaaccggaa attaagcgcg gattcgaata
     6361 ttctgactgt tgggtagacg acgcccgtct ggtactcgcc aacgcccaga tggtggtacg
     6421 taaaggcggt gaagtgctta ctcgtactcg tgccacgtct gctcgccgcg aaaacggcct
     6481 gtggattgtg gaagcggaag atatcgatac cggcaaaaaa tatacatggc aggcgcgcgg
     6541 cctggttaat gctaccggcc cgtgggtgaa acaatttttc gacgacggga tgcatctgcc
     6601 ttcgccttat ggcattcgcc tgatcaaagg cagccatatt gtggtgccgc gtgtgcatac
     6661 ccagaagcaa gcctacattc tgcaaaacga agataaacgt attgtcttcg tgatcccgtg
     6721 gatggacgag ttttccatca tcggcaccac cgatgtggaa tacaaaggcg atccaaaagc
     6781 agtgaagatt gaagagagtg aaatcaatta cctgttgaaa gtgtataaca cgcactttaa
     6841 aaagcagtta agccgggacg atatcgtctg gacctactcc ggcgtgcgtc cgctatgtga
     6901 tgatgagtcc gactcgccgc aggccattac ccgtgattac acccttgata tccatgatga
     6961 aaacggcaaa gccccgctgc tgtcggtatt cggcggtaag ctgaccacct accgcaaact
     7021 ggcggagcac gcgctggaaa aactgacgcc atattatcag ggcattggtc cggcgtggac
     7081 gaaagagagc gtgctgccgg gtggtgccat tgaaggtgac cgtgacgatt acgccgcccg
     7141 cctgcgccgc cgttacccgt tcctgaccga atcgctggca cgtcactacg cgcgcactta
     7201 cggcagcaac agcgaactgt tgctcggcaa tgcaggggcg ataagcgacc tcggtgaaga
     7261 tttcggtcat gagttctacg aagcggagct gaaatatctg gtcgaccacg aatgggtccg
     7321 ccgcgccgac gatgccctgt ggcgtcgcac gaaacaaggc atgtggctaa atgcggatca
     7381 acaatctcgt gtgagtcagt ggctgatgga atatacgcag cagaggttgt cgctggcgtc
     7441 gtaaggatga tgcccggttg cgaagtgcaa ccgggctatt tgaattcctt agactattca
     7501 ttgtaaataa ttgaataacg gccccagagc taaaatcgcc aaacttaagc acgttaccca
     7561 atcgccccac ttcttcctat ggcttctatt tttgctacgc cactggcaat aatatcccaa
     7621 cgcaaaacca agctgggcac taatcgggcc gacaaaatca gtccacggct gcggccaaaa
     7681 aggggaaaaa ttcaggggaa gccagacaaa tgtcagaaag aaatttcgga tattttccag
     7741 caacgctgat ttctctggag aaatataaag aatgaaattc gagagaatta accagaaata
     7801 gaccggagaa aaaaccagcc agcaatgtag cctgtttttt aattcaggag gcgttctgcg
     7861 ggagaattgc caaccgatgc cacccattac actgaaaaaa agtgcatatt caaccaataa
     7921 ctcaagttgt aattcctgaa gagaaatacg cacaaccagg agcggaaagg agaatattat
     7981 ttcagccaga aaagttaacg tgaatataag tacggctaag gcaataaccg tcggccatac
     8041 caataatggt acgataacaa aaccgaaaat tacagggtaa atataaagtc tgattttatt
     8101 catctttaat gtcctgatga ttcggatgat gcgtcataca tggcacataa acactcatca
     8161 atcatccact cgtcttccca ggcctgatac tgtgtgccct gttcggactg cttaagcgga
     8221 ttctcaatcg cctgccgcgc gcgttctttc aggatggatt gattattact agcccaagta
     8281 ttagtttgag tggtaatggc tttatcgata tcgtcctgat tatctctgat ataaccgtca
     8341 cgatacgtat tcaataaaca ggcaagagac gctgccgacc aaaatccggg aactgaaata
     8401 atggcatcaa gcaatggcga aagagcgata tcggcacgga aaaacatttc cagcttgtac
     8461 tgtaattcga cagcctcatc cccggatacc caatcagtca tatactggca agcaaattgc
     8521 tgcaatattt cccgttcatc ctcacgccag ttttctttac tggcaaggtt gatacgccgt
     8581 agagaaaata cttcgtgaat attgtcaaat tgcccctgcg caacaaactc cagtaaacaa
     8641 ggcagaaaat aacgaacctc atcgctgttt tgcggatcag gtcctggact actattccag
     8701 gcattgatta aggaataagg aatcgctcgc aacgatgtat tccgtaatgc tttttgttcc
     8761 tgttgactca gacaatattc acaacaaacc acaaattgct gcggcgccgg ataacgacga
     8821 aaaatatgat atatctgctc aattaatgcc tgcatggcag gagtggggtg cataattacc
     8881 ccctcgcccg ctgatcgtgc ataaacggct gatagataaa aagggcattc gccgcgttca
     8941 ggaaaatatt aatgatatcc atattgtaat cctgcttaat gcaacgtttt atccatcggc
     9001 acatagccca catcttccac caaactctgc ccctgcggcg ttaaaaacca ctccaccaat
     9061 ttttgtgtct ctgacgtcgt attttcccgc gttaccatta aggcatcgac gatataggga
     9121 tatttgtcgt tgcgaatatt ttccgcagtc ggtgcaatac cgttaatcgc tagcagtttt
     9181 atatttttat cggcattcat ttgtgttgcg taatagcgga aggtgtagcc tattgcgttt
     9241 tttgtattac ggtattcggc aacaacgtta atcattccct ccatcacgct tgccacttcc
     9301 gtttcctgcg gcgagatcat gcggacattt ttcatgacct gcgatagcat caccgtctgg
     9361 ctgccagagt cttccgggcg ttgccaggtc tggatctcct gatcgtcacc gccaaccgcg
     9421 cgccagttgg taattgcgcc actgaaaatg tcacgcacct gttgttcagt cagggaatta
     9481 accggattat ccgcattgac gatgaaaaca aacgcttcac gggcaaatgg cgtgtacagc
     9541 aaagtgacgc ccgatttctc agcgcgtttt ttttgcccat cggaaggttg cgccacgaaa
     9601 ataatatcgg catcaccgtt aacgattttg ttatatgctt cttgcgtacg agagttatcc
     9661 agataatccc aaacgtgaaa atcctctggt attacactta atgcataaaa tgcagaggca
     9721 taaatggggt acgccgccgt agcgccatcg agtcgcggcc agttttgcgt aaattgaatt
     9781 tgtggtttac cacgcagtgg cgtcagttga ttattaagtt tatcgggtcg ccaggcccag
     9841 gtatcaatat tttcacgcac accgacaccg ggattaagct gtttatataa ataagcctga
     9901 tacccagcga caaaaccaag taagagaagt agaaacagga gtaagttacg gcaacgtagc
     9961 ccggacgtgc cggttaaagg atgccttgtc ggccagccgt atcccagcga aaaactgata
    10021 tgcgcgccaa cgggtataat gactatccac cagtagtcgc cactgattaa tgagagtaac
    10081 aaaacaccct gccaggaaag cataaggttt gccaaccagc ccgatcccat ctggcgaaat
    10141 tgataatctg ccagatgtag cgcgattatc cagccaacca gggttaatag cgcgggtaag
    10201 agaaaaaagc attgtcgcca ggcggctatt tctctttttt ctggtgcgct atcaccagcc
    10261 cacacgcgac cagagaaaaa tatgattatc gcccccagcg tcagcataaa gacgttgagt
    10321 agaagataaa ttgttggcgt aaagagtaaa ttgcaaacct caagaatagc ggcaagcccg
    10381 gcccccagca tggcaacaac cgctgccaaa aattgcgcca gtatggggat gccaaaaaaa
    10441 gtcattacca gcgaggtcaa aatccatttt ccgctttgca ttattctttc cattcttgct
    10501 gaatggcgaa attatactcc ccgataaccc tcgccccctc tggacacttt tcctaaataa
    10561 aggcgaaaaa aacgggacct tttggccccg ttctgttttt tagcgaactt ataatctcac
    10621 cggatcaata tgccaaatat aatcggcata ctctttgatc gtacggtcag aagagaagta
    10681 acccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgac gctcgtagag
    10741 ttcgtcaact ttatcctgac aatcgacata gctgcgataa tccgccagta cctggtagtg
    10801 atcgccgaag ttgatcagcg aatcgaccag atcgcgatag cgacccggat cttccggact
    10861 gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta
    10921 ttcacgcggt ttgtagccct gacggcgcaa ttcttccact tcttccgctg tgttaccaaa
    10981 gataaagata ttgtcagcac cgacatgatc cagcatctcg acattcgcac cgtccagcgt
    11041 accgatagtc agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt
    11101 ccccgccagc gaaatctgtt cagacagatc ggccgccgga atgatcaact gcgccaggct
    11161 aacactgtag ttcgggatga acacgacttt cagcttgtcg ccaatctgcg gatcgttgtt
    11221 gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga
    11281 agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt
    11341 gatgcggtta tagcgggtga tcacgtgcaa cacattcatc aattgacgtt tgtattcgtg
    11401 aatacgtttg atttgcacat cgaacagcgc tttcggattt accaccacat tcagctgctg
    11461 ggcgatatac tccgccagac gctttttgtt ctccagcttc gcctgatgca ccgcgtgatt
    11521 aaccattggg aaatcacagt gttgttgcag atcattaagc aggctaaggt cggtgcgcca
    11581 gttgcggccc aggtgttcgt ccaacacggc tgaaagcgat gggttcgcta ccgccagcca
    11641 gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa
    11701 gtcggcaaat agcgattgca ccatcaggtt agagtgcagt tccgatacac cgttaacttt
    11761 gtggctcacc acaaccgcca gccaggccat acgcacacga cgaccattgg attcatcaat
    11821 gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag
    11881 gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat
    11941 atcgactggc caggtttcca gcgcctcgct cattagcgtg tggttggtgt aggagaagac
    12001 ctgacaacac acttcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg
    12061 catcatctca ggaatcgaca gcaccggatg ggtatcgttg aggtgaatcg caattttatc
    12121 cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt
    12181 cgaggaaacc aggaagtatt cctgacgcag tcgcagctca cgcccggagt aggtggagtc
    12241 gtccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa
    12301 gtagtcgccc tggttgaatt taccgaggtt aatttcacta ctggcttggg cactccacaa
    12361 acgcaacgtg ttggtcgcgt cggtgtcgta acctgggatt atctgatcgt aagctactcc
    12421 cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc
    12481 aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag
    12541 ccagtagtca ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta
    12601 gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcgaggaa
    12661 gcaagccgcc agacggccca ggccaccgtt accgaggccc gggtcatttt cttcatcaat
    12721 cagctcttcg agatttaatc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc
    12781 tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta
    12841 aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag
    12901 acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg
    12961 atcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct
    13021 aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt
    13081 caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc
    13141 catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta cgaaagcacg
    13201 tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac
    13261 gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg
    13321 ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc
    13381 aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc
    13441 gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg caaggaaacc
    13501 ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag
    13561 accaggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac
    13621 tgcaaaaagc ggcactttat cgtcaacctt aagccccatt gcgatttgta actggcgctt
    13681 attttccgct ttatcttcca acgtatcgcg ggtgtaacgc gaggccaaca gtaagtccgt
    13741 ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg
    13801 gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg
    13861 agcgtaggtt gggctgactg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa
    13921 agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat
    13981 gtcattcatg tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca cagtaaacac
    14041 cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc
    14101 gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc
    14161 aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc
    14221 gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac
    14281 cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac
    14341 tacctgcgca tcggtcacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc
    14401 gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc
    14461 ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt
    14521 ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga
    14581 tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca
    14641 caagcacgat cgatgacgca gcgacgcaga cggcacgagc gacctaccca tacttccggt
    14701 aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg
    14761 gactgcacca ccaccgaacc ggagatcaca caaccgccgg ataccagtga gttaagggtc
    14821 atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag
    14881 gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg
    14941 aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc
    15001 gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg
    15061 atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcgcg atcgtcttct
    15121 tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat
    15181 ttgcctggat cgttcggcat tgacggcggg ttagctggtt tttcaacgaa ttcgataatt
    15241 ttatcgttct catcaaccgc cataacgcca aacgcggagg cttcttcaat cggtactggc
    15301 atacaagcaa cggtgcaacg tgcgcctttt tcgacgtggt cgataagcat acgcgagtag
    15361 tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg
    15421 ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc
    15481 attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac
    15541 cagccgcgct ggatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata
    15601 cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg
    15661 ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt
    15721 cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgtgccaa cattaagtga
    15781 tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca
    15841 atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg
    15901 ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt
    15961 taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc
    16021 actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt
    16081 cttcccacca gcgattctcc atcaaagcgg gaatgcgctt gcgcagatgg attaacgcgg
    16141 cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat
    16201 cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta
    16261 acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc
    16321 gccgttcaac cagatcaaga gtaccgccta acccttcttt accatgattg ttactgtaat
    16381 tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc
    16441 aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg
    16501 gcagacgatc attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc
    16561 ccagaggcaa atcataatgc agccagaaac gacgggctgc atcgcggaaa tgatcgttcc
    16621 actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg
    16681 gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg
    16741 gcgcatcctg acgaaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac
    16801 catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa tctaccaccg
    16861 ccggatgact caaattgagc gtgttgccac aaccggtcca gttgtgataa tcgccgtctt
    16921 ctcttatcca ataatagcta cggttgtcga tcccacgcag cgagaataac gggccgtcga
    16981 ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat
    17041 gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag caggcatacg
    17101 ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttacta agccccatac
    17161 gttgcagacg tggttcactg gcaaattgtg ccaccggcag cagttccagc gcggtaatgc
    17221 ccaactgttt caaatagttg atcatcaccg gatgccccag ggctttataa gtgccacgga
    17281 tctcgaccgg gatctccggg tgcaggtacg ttaatccttt gacatgggct tcataaatga
    17341 tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaatca
    17401 ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg
    17461 cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca
    17521 acaacttcgc cgggttaaag cgatgcccct cggcaggttg ccaggggcca tgaactcgat
    17581 aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt
    17641 gccctggcaa gtcatagcga tgttcctggc cattggcatc aaagacacac agctctaccc
    17701 gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga
    17761 ggggagtggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt
    17821 ggccagaggt ggtagcgtca ggctcagtga atgttgacga ccgtggctgg caatctcatc
    17881 gctgtgtact gcgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt
    17941 gaggatttca cgccatttac ccggctggtt aataccgaag cgataatcat gacgcggcac
    18001 cggtgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat
    18061 cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc
    18121 cagttcatgc attgctttat ggtggcggta ggtgtggttc agatcgcgca ccagacgctg
    18181 gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg
    18241 gttccactcg cggccctggg caaattcgtt ccccatgaac agtagtttct tgcccgggaa
    18301 tgcccacatc cagccgtagt aggcgcgcag gttagcgaat ttctgccatg cgtcgcccgg
    18361 catccggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac
    18421 gaagttttca gtgtagttgt agagcatccc gaaggtcagt ttatcgtgat gatactgacg
    18481 ataaatcggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt
    18541 gtaccagaag cccagaccgc ccatatcctg tggacgagaa acgccaggga aatcggtaga
    18601 ctcctccgcc attgtcactg caccggaaac ctgctcacca agaatacggt tggtattacg
    18661 caagaattca atcgcttcaa gattctcgcg accgccaaat tcgttcggga tccactcccc
    18721 ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc
    18781 aataccaaaa cgttcaatcc agtaaagcgc gttaccgaca aggaagttac tgacttcacg
    18841 gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcgc gcggatcgct
    18901 gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcgg tcgggaagtg
    18961 gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata
    19021 acggaagtcg tcgcgagtac caaaacggcg ggtcggtgca tacaggccgg ttggctgata
    19081 accccaactg ccatcgaagg gatgctcgtt aatgggcagt agctcgagat gggtaaagcc
    19141 catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt
    19201 gttgtcggtg tggcgacgcc aggagcccag gtgaacttca taaatagaga ttggcgcatc
    19261 aaactgattc gcttttttgc gctcttcagt ctgcacaacc ttttccggca gcccgcaaat
    19321 aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg
    19381 caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg
    19441 gataaacagt tcccagattc cgctctcttt acgcaggcgc atcggatgac ggcgaccgtc
    19501 ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagaaaa
    19561 acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg
    19621 taggtgagta ccttcagata atagccaggc atccatttcc tggattagcg ggccaaaacg
    19681 gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa
    19741 aaaattctta cgtcgcggaa tgacaccgct aaagaatccc cgtgagtcga gacactccag
    19801 ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa
    19861 aagggcacgg acttccagtc ccgcggtggt tttatgcatc cccagtacgg aaaaaggatc
    19921 cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct
    19981 tgtcttcctg ttttattgtg tcaccccatc caactgaatt tttgacttct ggttgtgaca
    20041 tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag
    20101 gtaaggctgt gaatactcgt gtattccgcc acccttaaag aatagccaat gctctattta
    20161 actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta gcaggggcgg
    20221 catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc
    20281 ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt
    20341 cccatattca gcttacgcag acggcctacc ggcgtggtca gcgtgccggt aacggcagct
    20401 ggggttagct cacgcatagt gatttcccga tcgtttggaa caactttcgc ccacggattg
    20461 tgcgcagcca gcagttcttc cacggtcgga atagacacat cttttttcaa tttaatagtg
    20521 aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt
    20581 acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc
    20641 tgaccgttat caagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca
    20701 aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg
    20761 agaatagcag aggacgggtt cgcgagttca tcagccacat ggccatacag atggcccatc
    20821 tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg gtaggttgca
    20881 acggacaccc aatcaacaag atcattggcg aataaaccac ccagcgacat caacatcagg
    20941 cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg
    21001 acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagatgat
    21061 gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg
    21121 gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca
    21181 aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg accaagctga
    21241 gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc
    21301 atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt
    21361 ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcctggca catctttcac
    21421 catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta
    21481 atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg
    21541 agttatgaat gaaatcattt ctgcaacagt tttattgatc ctgattatgg atccgctcgg
    21601 aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat
    21661 catggtgcga gagttgctta ttgctctcct tgtgatgctg gtgttcctgt ttgcgggcga
    21721 gaaaattctg gcatttctta gcctgcgggc agaaaccgtc tccatttctg gcggcatcat
    21781 tctgtttctg atcgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct
    21841 tccggcaggt gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg ccgggccgac
    21901 tattctcgcc acgctgatgt tgttgtctca ccagtacccg aatcagatgg gacatctggt
    21961 gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt
    22021 tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct
    22081 ggtgatgatg gcaactcaga tgttcctcga cggcattcga atgtggatga aggggtaagg
    22141 ttcaaacagg tgttgcacac aaccagacgc ggcacctgtt ttattacctg aactgataat
    22201 gaccttcgcc caatcggata acccttccct cttcttctgc gcggttcagc cacacactaa
    22261 tttgttcgat ggttaaacca gtacaagacg ccagcctttc aatacttatc gattcctttg
    22321 caaggatcgc cagttccgcc acaaagagtt gataaaaatc gacaggggta tgcgcgggca
    22381 ttactgcgta atttgcgtca tcttcgtgca gcgaaaacag ttctggttga gcgttacgga
    22441 gtgctctgtc atacatcagc ccaggaacgt ctggcagtct gatcgtcaca ttctcagcct
    22501 gttcagcact ccatgacgcc ccattttcta ctaatcgtga attggctgat tgcatatcct
    22561 gatttggttt aacctgcatg ggcagccact gatgttttaa tgcctccatc gccccggtta
    22621 tcgttccacc ggtcattccc gcacgtacaa ccattgcgct ttcagcaagg caataaatat
    22681 atttattccg cgccatcgca ttgccgacgg tgaaacgtac ctctgggtaa aacggcgaaa
    22741 tcaacaccag gttgcctgct ataagccctt cacgccattt cactaacgta ctcgttttta
    22801 acaagctatc agctaatacg ccaacggcag ttcccccgac ctccagagcc gaggccattg
    22861 cacattcatc aatacctcgc gcaccaccag agataacgca aatcccctgt tgggccagtt
    22921 tcgcggccag ttgttgggta tagcgcaaat cgtcagtcgg agcatctcgc gagccaacaa
    22981 tcgccatacc ttccgcttgc agtaatgctt tattcccgca gccaaataaa acgggaggtg
    23041 catccgttcg caatcggttt ttcaagcgaa cgggataatc ggcatctccg cgggttaaga
    23101 tccataaacc ggcgcgttgc cacttatcta cagccaacgc cagactatga ccacgcgcca
    23161 gcaagccaag aattcgctcc tgagaaatac gcggatcgct ccagcctgta agtttctctt
    23221 gcggttgcgg caccagaagc tcggcgggac tgatacgttg gtgcttcagc cataatgcaa
    23281 acttccccca ttcactatta ctaagaggtt tatactcact cgccgccgcg cgagaaaaat
    23341 cgctggttag caggagagtt gcttgagcat tggctgaaag attcatcaat tttttaccga
    23401 ggtagaagca agggcaagag gataaaccgt cgggcaacct gcctggcgta acagtgctgt
    23461 cagaactgtc agcgtccatg cagaatccac gatatcatca accagcaacg ccggacctgg
    23521 catcaaagga ggggtaatca caaacgcccc gtcgagattt tgacactggt gaaaacggtt
    23581 ttgctgcatt ttctgcggtg gattgtccac gactttttca atggcatcaa taaaaggtaa
    23641 gccaagtttc gccgccagtc gccgggcaaa atcaggaacc aggtcgaggt gccttaatga
    23701 aggaacgcag catacccagg tgggttcagg atgcggattc cagcgttggc gaaccatttc
    23761 cgcacatgcc tccaccagct catcactgaa gcgccccgtt tttttctcct gggctacctg
    23821 ctttccccac ccggaatctc cccaacgaga aaggattcgc cccgtagatc cttgtaaacc
    23881 cgcaggcaag ttccctttaa atccgtattg cgtaaaagcc ccagatgcca cctgtttatt
    23941 gagatttaat ggcaggtcag cgtgtttaat aaataaattt gccgcatgga gtaagtcgct
    24001 atcaatgtcg ggcgacaaca gcggatgctg aagacaactg ctacatttac cacaacgttc
    24061 ggcacttggc tcatcgagtg cgcggcgcag aaattgcatt ttgcactcct tggtcgtgat
    24121 atagctttca acgtctgcca actcactttt tctctgattc atcaaatggt taattcgttc
    24181 atgatcagga gaaaaactga cgatagttct gcgccataat ttctcggtat acaccacagg
    24241 cgatggattt tcagctacca ataatttcag tgctttttct atttgcccat aacgaagatt
    24301 cgtccgttgt tcaatgcctc gtagggtaag accgtcattc tcgctaagta cgttgagtat
    24361 ttcatgaatt tgcgcctccg caggaaaggc gctttcacgg aagaattgat gaatagcgcg
    24421 gtcttcacca ccacaaagca atatgccaac tgcggaatct atagcacgcc cggcacgccc
    24481 cacctgttgg tagtaaccga caatagaccc cggcatctga taatgaatga cgaaacctaa
    24541 atcaggttta tcaaatccca tacctaatgc cgtcgtcgcg accagcactt tgattttatt
    24601 tgccagcagt gcctgctcca gatactccct ggcagtatta gaatcttccg caccttcaca
    24661 agtcacgccg ctgtaataag caaatgcact gataccattt ttccttaacc actcggcgac
    24721 aagttcagca tcgcgagtcg tcagggtata aacaattccc gatttggaaa actgagggat
    24781 aaccgttgct aaccatgcca gacgggatga ctgttctcct aagactaagg catcaagcgc
    24841 caaactttcg cgagccagcg ttccacgctg aatcacaatg tcacccaatt gctgacggat
    24901 atcctcaacg acacggttat tcgctgtcgc ggttgtaccc agaatagggg tattcgcagg
    24961 cagttggcgc aaaatatcta atatacgtcg gtaatccggg cggaaatcgt ggccccaatc
    25021 agagatgcag tgcgcctcat cgaccaccag taaaccgatg cggtcagcga ctggatataa
    25081 aaccgtttca agaaaatcct gattcgccaa ccgttcaggt gaaatcagca gacaatctac
    25141 gccaccttgc agcaacttat cgctaatgcg ctgccattct tccctgtttg tagaattcaa
    25201 cgtttcagcc gtaataccta accgttcagc ggcggcaacc tggttacgca tcagggcaag
    25261 caaaggagag atgataattg tcgggccagc gccgcgatcg cggaagattt tactggcgat
    25321 gaagtacacg gcacttttcc cccagccggt gcgctgtaca accagtagtt ttcgccgctg
    25381 attaaccacc gcatctatag cttcccattg cccttccctg aaagtagcgc tggtgttact
    25441 taacatccgc tgtaacagca attcagctcc gtgtttctcc atcatcttct ccctcgtccg
    25501 ttaacacagt gattagaacg gataatgttc cggtcttttt gcgagagaga tcaaaaagat
    25561 gaagacagga gaaaagagaa atgaattact gcgagcaagc tcgcagtaat ttgtcgcagt
    25621 aattaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt gccgaggatg
    25681 gtttccatca tggtccaggt tttaagcgtt tcggcttcgg tcgcgccggt aaatttaccg
    25741 aacaaccaga aacccgcgtc gttaacgtgg ctgacaacaa tcgaaccacc ggcgatacaa
    25801 atcgacagtg ccgccatttg cgcaccggag tagttcagtt gttcaatgac cggcattacc
    25861 aatcccaccg ccgttaaaca ggcaacggta gcagaaccct gaatgatgcg cactgcagct
    25921 gccagcacga agcaggtgat ggcaatcggc aggcccatgc cggttaacgc ttcgcccagt
    25981 gccggaccga caccagagtc aaccagcacc tgtttgaaca cgccgcccgc accaatcacc
    26041 agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac tttgtctttt
    26101 ggcataccct gacgcattgc cagaccgtaa atcgccacca ggcaagcaac cagaatcgcg
    26161 gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagaaccttc tggcacaaaa
    26221 cgcgcggcaa tggttttcag ccccaccagc accagtggca acaggatcag cgacaggctg
    26281 aatccgaagg acggcatttt gccttcgccg agatgcggtt cgctgatgtc gtcaggaata
    26341 tgcaactcca cgtagcggct gatgaaatta ccccacagcg gcccggcaat aatcattccc
    26401 ggaattgccg cgcacaggcc aatcaggatc atccagccaa agtcggcgtt catctgcgat
    26461 gccagcagca ttggcgctgg cccaggcacc aggaacgcag ccgctgccgc cacgccggcg
    26521 aataatggga ttaccagctt caccaggttc gtgccggtgt ggcgcgccat tgagaaagca
    26581 acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca aaccagcccc
    26641 gcaaggccga tggcataatg cacgcggctg tgaccgaagg atttgagcat tttgacggca
    26701 atctgatcga ccgcgccggt ttcatgtaag atcttaccaa acatggctcc cagggcgaca
    26761 accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc tgcgatttta
    26821 tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccattaa agccaggaaa
    26881 gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc tactgctgtt
    26941 aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc aatggtgctt
    27001 gccacaacac cttccagcgg ttgatcaata tccaccacca gtacatcggt ttcgtccgca
    27061 cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa gaaatggcct
    27121 ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc ctttcaggta gatgaaagag
    27181 agattaggat taccttcacg cagcaagtcg cgatagtgtt ttttcagtgc agaacagacg
    27241 atcagcgata ctttattggt gcgctgcata gcaaacgcgg cgtcgttcag cgcctgcaac
    27301 cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc gatattgcag
    27361 cgcggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc cacttcactg
    27421 gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg gtgatcatgg
    27481 ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta cgggtaactg
    27541 ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg tgccttaagt
    27601 gtataagtgt gagctacttc aaatttgtag gcttaaatag atccgcccgg tgacaaggtg
    27661 aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc cagcaggcgc
    27721 tcagcgccaa tgctgcccat ccgctcacgc ggcgtcagca cactcgccag ccgtggctcc
    27781 atcacctgac caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc aggaactttc
    27841 aaccccagac gctgacattc aaacgccgcg ccgaccgcca ggtcatcatt agtacagaac
    27901 acgccatcca gctgcggata ttcccgccgc gcctggcgaa tcagttcaat accggaagag
    27961 taagaagaag attgctcaac catcacgcta tacggcacca ggcctgcatc cagcatcgcc
    28021 tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgtgcgcc gagataggca
    28081 atgtggcgat gcccgcgagc aataatggcg gtggtcatct ggcgtgccgc ttcaaagtta
    28141 tcaaaaccga cggcgatatc gagacacggc gacttactgt ccatcaactc caccaccgga
    28201 atccccgcca cttcaatcat ctttaaggtg cgtggcgtgt gggtacgttc agtgaggatc
    28261 aggccgtcga tattccatga gagcattgat tcgaggcgtt cttgctccat ttccggttta
    28321 taaccgtagt gcgccagcat ggtttgataa ccgtgcgcgt cggtgacact ttcgattccg
    28381 cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc aatcgcccgg
    28441 ctggtggcgt tagagagtat atcgggcgcg cgattgggaa tataacccag ttcatcaaga
    28501 gcggcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg taaaaaacgg
    28561 ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac gggtcttttc
    28621 tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca cggacgcatc
    28681 acaaccttcc tgattcctca ggaaggtttg tgagataaat ttagactggc ggtaaatcga
    28741 acagcaggat ttcactgtcg ctatcagcat ggatggaaat cgcctgctca tcccagattg
    28801 ccagaccatc gctggtcgaa gcttttacgc cgttaatggt gacattgcct ttcaccacct
    28861 ggatccagat gcggcgttca gcggcaatct gatgcaccga ctgctcatct ttcaccagcg
    28921 cccaacgata cagttccata tcctgatgca ctttcaatga accatctcgc gcatccggcg
    28981 aaagcaccag ttgtttgccc tgcacggcat cgaagcgacg ctgttcataa cgcggcgtaa
    29041 taccgttttc ttcaggcatg atccagatct gatacagatg cagacgctcg gtgctgcttg
    29101 ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac tcgcccgccg
    29161 gaacctgctc tttattgccc atgctgtcct ggtgctcaac agtaccttcc agcacgtagg
    29221 tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctgct tcaataacgt
    29281 cgtcgttaat cacgcgcagc gcagagaagc ccataaagtt cggatcgtaa tagttggcaa
    29341 aagagaaagt atgccaggag tccagccagc catgatttgc atgaccgcgt tcatttgctt
    29401 tgcgtaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt agtgtggacc
    29461 cgatccgcct gggatgatag agggtgaaaa ttgacccctc tgttcaaaaa aattgaacta
    29521 ttcgaggggc catcaaactt acctcgcgag actcactgcg gagggagaag cttgctcaaa
    29581 accgcgttca aggatttcca gattcgtaag aacttcagat tccttgacgt aatttggcgc
    29641 accgttggta atggtttgat acaacgcatc ataaacgcgc ccataatcgc ccatctctgg
    29701 cttcatctct tctcttaccg tcacgccctc gtcattgaca tactccagca caccgaccga
    29761 atcatccgct gcgaatcctg gttcgcccgg cataatatta gccttcaggc tggtttcctg
    29821 ctggtcgata ccgtatttaa taaacgaacc tttcttaccg tgaacgataa atttcggata
    29881 atcgattttc accagatggc tggttttgac gatggctttt agatcgccat aaaacagctg
    29941 cgcttcaaag gtgtcgtccg gattggcttt attacgcagg ctgcggatgt cataagcgac
    30001 gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac caaggccata
    30061 gaacgcgcca tcctgcggca gccccggttt ggtttctgcc accgggcggt aatagtcaaa
    30121 atggctttcc acttcgacaa tctctcccag cttgccgctt tcaatcgctt ttttcgctgt
    30181 caggaagcag gaatcaaagc gacgattctg atacggcgta acgatcagcc ctttgctttt
    30241 cgccagtgca aacagctctt tcgcctgcgc aattgtcgga gtgaacggtt tttcgaccag
    30301 cacatttttc ccggcttcca gcgcgcgctt cgcgtactcg aagtggctgt ccgcgtgggt
    30361 gcagacgaca accagcttaa catcgggatc gtttagcact tcgtcgagat cgctggtgaa
    30421 atggatatgg gaataaatgg gggcctgttc ttccggcttc gcatggcggc gaaaaatatg
    30481 cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac gggtggtgct
    30541 tttgccgaag ccaataaagg cgcagttaat gaccatgatt tcgtcctttt taaggtggtt
    30601 atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa cgaacacaag
    30661 cacttcccga ggataaattg agaacgaaag atcaaaaaaa aagccagcac ccggctggct
    30721 aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc gagggtcttc
    30781 ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt tttgcaaaaa
    30841 aatgcatttg actcgcattt gaaagtcaat gatgttgaaa gggacattta ccccaaagag
    30901 gacaaaggaa tgagtgagat agtaatacgc cacgcagaaa cacgcgatta cgaggccatc
    30961 aggcagattc acgcccagcc ggaggtgtat tacaacacgc tacaggtgcc tcatccttca
    31021 gaacaaatgt ggctggaacg actcaccgct cgccctggca tcaagcagtt agtcgcctgt
    31081 attgatggaa tcgtcgtagg ccatctcacc attgacgtgc aacaacgccc acgccgcagt
    31141 catgttgccg attttggtat ctgtgtcgac tcccgttgga agaaccgcgg cgtcgccagc
    31201 gccctgatgc gagagatgat tgaaatgtgc gacaactggt tgcgggtaga tcgtatcgag
    31261 ctaacggtgt tcgtcgataa cgcgccagca attaaggtct ataaaaaatt tggctttgag
    31321 attgaaggga ctggcaagaa gtacgcatta cgaaacggtg aatatgtcga tgcgtattat
    31381 atggcgcgtg tgaaatagag aatttgcaat ctacgtttgt catttaagcc ctcacctacc
    31441 gggagagggc ttaagatatt aataccccgc cgttaaatca tccaccgagc gcgggtcgga
    31501 tgcaccgtac agctcaccgt ccggcccaac cataatgctt tgtgtactgc ccatcgcctc
    31561 tttcagcgcc actttctgac cttttgcttc cagcagcttg atcgtatccg ggctaaaccc
    31621 tttttcgaca cgcagctcgt ccggcaacca ctgatggtgg aaacgcggcg cattggtcgc
    31681 ttcggcgacg ttcattccat aatcgatgct attcaccacc atttgcagca cagtggtgat
    31741 aatccggcta ccgcctgggc ttccggtgac cagccaggtt ttaccatcct tgaccacaat
    31801 ggttggtgac atcgacgaca gcgggcgttt attcggcccg acggcgttgg catcgccgcc
    31861 caccagcccg taaacgttcg gtacgcttgg tttggcggag aaatcatcca tctggttatt
    31921 gagcaaaata ccgctctcgc ccgcgacaat gcccgtaccg aaggtggtgt tcagtgtata
    31981 ggtcaccgcc acggcgttac cgtctttatc cactactgag taatgggtag tttgattact
    32041 ctcataaggc gcaagcttgc caggacgaat ttcgctggat ggcttcgctt tattgatatc
    32101 gatttgatcg gcaatagatt tggcataggc tttattggtc agcgcctgcc acggtacttt
    32161 gacaaaatcc gggtcgccaa gatattccga gcggtcggcg taggcgtatt tctcagcttc
    32221 tgccatgatt tgcatcgcgt cggcgctgcc aaagccgtac ttctgcatat cgaagttttc
    32281 cagaatattg aggatttgta cgatatgaat accgccggaa gatggcggtg gcatggagta
    32341 aacctgatac ccgcgataat cgccgcttat cggtgtgcgt tcgaccgctt tatatgccgc
    32401 taaatcttct ttgctgatca agccaccgtt tttctgcatc tcctgggcaa tctgttccgc
    32461 tatcgtgcct ttgtagaatt catccgggcc gttttcggca atcatctcca ggctctttgc
    32521 caggttcgcc tgcaccagct tgtcgccctt tttcagcggt tcgccctctt tccagaagat
    32581 agctttgctg ttttcgtggt tcggcagcac ttcactgccg taggttttga gatcgtcagc
    32641 cagcgcgtcg ttaacgataa aaccatcgcg tgccagttta aacgccggtt gcaccacttt
    32701 attcaacggc atggtgccgt atttatccag cgccaacgag aaacccgcta ccgtaccggg
    32761 tgtgccggaa gccagatgcg aagtgagtga ttttttgctg tccgtgttgc cctgatcatc
    32821 gaggaacata tcgcgggtcg ctttggcggg tgccatttcg cggaaatcga tagccgtggt
    32881 attgccattt ttcgagcgga tcagcataaa accaccgccg cccagattcc ctgcctgagg
    32941 atgcgttacc gccagcgcgt agcccaccgc cacggcagca tcaacggcat tcccgccctc
    33001 cttgaggata tccactccca cctgagtggc agtaacatct acagacgcca ccatcccttg
    33061 tttcgcgcgt accggatgga agacatcttc ctccacacca taagaaacag gaggcgcggc
    33121 taccgtacta aaacaacttc ctgtgagcag agcagcaatg gccacccggc gtaaaaacgt
    33181 cggttttatc atcgttattc tccagagatt aaggggcaac cccagctaag cctggtatat
    33241 aactctgaat taatcatcgt tttgccggga agcgagtaaa cttaaagaat atcttcagag
    33301 gaggatacgc gatgaaacga cttctgcttt tgacggcact cctgccgttt gtcggctttg
    33361 cacagcccat taatactctg aacaacccta accagccggg atatcagatc cccagccagc
    33421 agcggatgca aactcaaatg cagacgcagc aaattcagca aaaagggatg ctgaaccagc
    33481 aactgaaaac gcaaacgcag ctgcaacaac agcatttaga aaaccagata aacaataatt
    33541 ctcagcgggt gttgcagtca caaccggggg agcgaaatcc cgcccggcag caaatgctgc
    33601 ccaacaccaa cggcgggatg ttaaacagca accgtaatcc ggatagttcg ttgaatcagc
    33661 agcatatgct gccggagagg agaaacggcg acatgctgaa tcagcccagc acgccgcagc
    33721 ctgacattcc gttgaaaact attgggccgt aaagttcgga ccaatcacgt caatcgcatc
    33781 ggtacagatg caatccacgc cccagcgcag caactctgcc gcgcgctggg gtttattgac
    33841 ggtataaacc agaattcgca gtcctgcatc tttcaactgc atcactcgcg ctttatcgag
    33901 caacttataa ttgagatgaa tagagacgca gcccagccgc gcggtcaatt ctcgccagtc
    33961 gtcgcgccat tcatcaagca ataaaccacg cggcagttcc ggtgctgcct gttgagcggc
    34021 ttctaaagca tcaatctcaa acgatgacag cagcggcggc gtcataccgg tccacagttc
    34081 gcgtgccgcc aacgccacca ttttgcccgt taatggtcca gtgtcagtgg tgggtttgat
    34141 ttcgatattc gccatcatcc cgtgtttgcg acagcgttcc gccacctgcg aaagcaacgg
    34201 cagtggctca cctttaaacg ctttgctgta ccaactgcct gcatccacgc gcagtaaatc
    34261 ctgccagttc agttcacccg cgacgcccca gccgttgctg gtgcgttcaa gattgtcgtc
    34321 atggagcagg aagatctcgc catctttcga taacttcgcg tcaaattcga tcatcttatg
    34381 accgtatttt gccccgacgt cgattgctgc cagggtgttc tccggggcca gtttaccgcc
    34441 gccacgatgg gcgacgatgc ggggataagg ccagttactc atactcgttg tcctgtttca
    34501 ccatcaaaaa gatgtagctg attttccggc agatgcagcc acagcgtgct gcctgccgtc
    34561 gggcgctcct gatgtgccag tcgcaccacc agcttctgtt cgccccaatg tccgtgcgcc
    34621 aggttatctg cgccgaggat ctccagcgtg tccatcacca gcggcacacc accttctgcc
    34681 tgcgagctta gcgctatatg ttccgggcga atgccgaggg tcattttacg cccgacatac
    34741 tgacggtagc caccgtttag cggcagcgca ataccgccat ccagttcgaa atgcgtacct
    34801 tcgttattca cacggccagc cagtaggttc atcgccgggc tgccgataaa actcgccaca
    34861 aacaggctgg cgggcttttc gtacacttca actggcgtgc caatctgttc ggcaacgccg
    34921 ccgttcatca ccattactcg ctgggcgagc gtcatcgcct caacctgatc gtgggtaacg
    34981 tagagtgaag tcgttttcag gcgacggtgc agctgttgca gttcaagacg catctgcaca
    35041 cgcaacttgg catcgaggtt agagagcggc tcatcaaata ggaacactgc cggctcacgc
    35101 acaatcgcac gccccatcgc cacacgctga cgctgaccgc cagatagctc acgcgggcgg
    35161 cgcttaagca ggccgtccag ctccagaatg cgcgccgctt ctttaacacg ttcggcaatt
    35221 tgttgcttgc ccatgccgcg aattttcagc ccccacgcca tgttctcctc tacgctcata
    35281 tgcggataga gcgcgtagtt ctggaacacc atcgcaatcc cgcgatcttt cggctccatt
    35341 tcggtgacac gcttgcggtc gatccagata tcgcccgtcg ttacccgctc cagtccggcc
    35401 accattcgca ataacgtcga tttcccgcag ccagacggcc cgaccatcac gataaattcg
    35461 ccatccgcca catcaagggt cagcggttta atgacctggg ttttgccatc ccagcttttg
    35521 gttactgcct gtaatttcag tcctgccatc ttatttctca ctatcgacca ggccgcgcac
    35581 gaaggcacgc tgcatcacta aaacaatcac tactggtggg atcagggtta acaacatcgc
    35641 cgccatcacc gagttccatt ccgttgtgcc ttcgcctgtg gcgatcatcc ctttaatccc
    35701 tgccacggtg gtgccgagat caacgtcggt aataatcaac aacggccaca aatactggtt
    35761 ccagccgtaa ataaaggtga tcacaaacag cgccgccaga ttggttttgg acagcggaaa
    35821 aacgatgtcg caaaagaaac gcattggcga cgcgccgtcg atccgcgcgg cttccaccag
    35881 ttcatccggt agggtcataa agaactggcg aaacagaaac gtggcggtcg ccgaggccat
    35941 cagcggcagc gttaaacctg cgtagctgtc gagcattttc aggttggcga tgacttccac
    36001 cgtcgggaag atacgcactt caaccggcag catcagggtg ataaaaatca tccagaagaa
    36061 gaggttacgt agcggaaaac gaaaccagac aatggcaaat gccgagagca tcgagacggt
    36121 aattttgccg agcgtaatgc tgaacgccat cacaaagctg ttaagcaaca tccgccagaa
    36181 cggcgcgcta ttcgtgccta ccccgtttac ccagatgttg tggatgtttt ccagcagatg
    36241 tgtgccggga ataagcgtca tcggcgcggc gtagacttct tgtttatcca gcgtcgccgc
    36301 gacaaacgcc acgtacagcg ggaagaggat caccgcgatc ccgaggatca gcatggtatg
    36361 gctgaatatt gtcagccacg gacggttctc aatcattggt aacgtacctt gctttcaaca
    36421 tagcggaact gcaccaccgt cagcacgatg acgaggaaca tcaacaccac cgactgcgcg
    36481 gcagacgaag ccaaatccag tccggtaaag ccttcacggt agatcttata aatcagcgtc
    36541 gtggtggcct gtactggccc gccggacgtg gcggcgtcga tcaccgggaa ggtgtcgaag
    36601 aaggcataca ccaggttcac caccagcagg aagaaactca ccggagcgat aagcggcagc
    36661 gcaatcttaa agaagcggcg aatcgggcca gccccatcaa tggctgcggc ttcgatcaac
    36721 gaacggggga tggattgcag cgcggcatag aagaacagaa agttgtagct gatttgcttc
    36781 cacactgagg caaacaccac caggaacatc gcctgaccgc tgttttgcgc gtggttccag
    36841 tcgtagccga actcagcgag aaaatgggtg atcagcccgc gaccggggtt aaacagaaag
    36901 atccacaata cggcggcaac ggcgggagcc acggcatacg gtagcaacat caaggtttga
    36961 tagaaacgac tgccgcgcac gatgtactcc accagcgctg cgaagaacag cgataccagc
    37021 aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg tccagaaggc gtcgagatag
    37081 tagctgtcgt gaaacagcgt gacgaaatta tccaggccga caaactggct ggagaaacca
    37141 aacggatcga cgctttgtag cgagtaccac aacgcttcgc ccgcaggcca gataaaaaag
    37201 ataacggtga tgatgagctg cggcgcgacc agcaaataag gtagccagcg cgagcggaac
    37261 accggacggg atgatggcat tactgttaat tcctgaactg tgccggatgc gcttcgctta
    37321 tccggcctac acgcgatgcc aggtcagata agcgaaacgc atccgacatt acactgatta
    37381 agacttcgtc gatttctcaa agcggcgcag taactggttc ccacgctcaa cggcagtatc
    37441 cagcgcctgc tgtggtgtct tcttaccggt ccacacgctc tccagctctt catccacaat
    37501 cacgcggatc tgcggcatgt tgcccagacg caaacctttg gtgaacggca acggtggctt
    37561 gttcagcatc tgacgcgtgg caatatccgc tcctgggttt ttctcgtaaa agccctgctc
    37621 acgggtcagg tcatacgccg ctttagtgat tggtagataa ccggttttct gatgccactc
    37681 ggcagcgttt tctggttttg cgaggaagtc gaggaacttc gccacgccgg tgtaagtttc
    37741 tttatcttta ccctgcatca cccacaggct ggccccgccg ataatggcgt tttgcggcgc
    37801 gtctttcgca tcggcgtcgt aaggcatcat gcctacgcca tagttaaatt tggcgtactc
    37861 gcgaatgttg gcaagagaac cggaagaggc ggtcgtcatc gcgcaatcac cgttatagaa
    37921 cttctcggtg gattcatcct tacgcccaac gtagctgaaa tcgcccttct tgttcatctc
    37981 ttcgagcatg gcgatgtgtt tcacctgctc cggcttgttg aactcaagca ccgcgtctgt
    38041 gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg ttccaggcgc taaagttttc
    38101 cagttggatc cagccctgcc agccgctggc gtagccgcac ttcatgccgg aggcttttag
    38161 ttttgcggca tagtccgcca gatcctgcca ggtttttggc ggctgttccg ggtctaatcc
    38221 tgctttcttg aaggcgtctt tgttgtaata gagaacgggg gtcgaactgt tgaacggctg
    38281 ggaaagtaag tgcccagttt tgctgtcgga gtagtaaccg gaaaccgtcg gcacaaactg
    38341 cgactcatcg aattgaatcc ttgcctcttt aaatacgtca tacaccggtt taatggcttt
    38401 cgacgccatc atggtggcgg tgccaacttc ataaacctgc aaaatagccg gagcgttgcc
    38461 ggtacgaaat gcggcaatcc ccgcgcttaa attctgttcg taattgcctt tataggtcgg
    38521 tacaattttg taatccgggt tttcggcgtt aaaacgttgg gccagagaat ccacctcttt
    38581 acccagttcc ccttccatag aatgccagaa cggaatggtc gtcactgcct gcgcatttgc
    38641 cattaaagcc agtccgagcg ccagtgctga agctgtataa cgtaacggtt tcattgatta
    38701 tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta tgctcgcgga gtaacatgac
    38761 atgctcgaat tacagaaaaa taactatttt gttacatttt taagatagta aggtgtcaga
    38821 aagatgacaa ggcggtgacg gcgcgggcga gggaaaatgg gagatggggc acggataagc
    38881 ggaaaaatag aaggtctgaa tcaaactcta cagattactc atcgtttcat gccggatgcg
    38941 gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc aatatattgc aactcaccca
    39001 gtaggcctga taagcgcagc gcatcaggca attttacatt tgtcacctgt ctcaaaggag
    39061 tcttttgact ccttatcaat caacgtgtta ttacccgcct aaatatgcac ttctcactgc
    39121 ttcattcgcc agcagcgcgt cgccggtatc ggagagcact acatgaccgt tttccagcac
    39181 gtagccgcga tcagccagct ttagcgcctg gttggcgttc tgctcgacga ggaagatggt
    39241 catcccctgc tcgcgcagtt gttcgatggt gtcgaaaatt tgctggatga taatcggtgc
    39301 cagcccgagc gatggctcat caagcagtag caaacgcggg ttactcatca aagcgcgacc
    39361 aatcgccagc atctgctgtt caccgccaga catagtgccc gctcgctgaa cacggcgctc
    39421 atgcagacgc ggaaacagtt catagaccca ctttatgcgc tcctggaact ggtcgcgttc
    39481 ggcaaaaaag ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga agacgcgacg
    39541 cccttccggg acaatcgcta ccgcttcgcg catgattttc gccgtctgcc agtcggtaat
    39601 gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc ggatcgccgc ataacgtgcc
    39661 gagcaaggtg gttttccccg ccccgttcgc gccaatgagg gtgacaatct cgccctgatt
    39721 gatatgcagg ctcacctcat gcagcgcctg gattttgccg tagtgggcgc tgactttgtc
    39781 aaaggacaac atgacttttt ccatcttatg cctcacctaa ataggcacgg atcacatctg
    39841 ggttattacg gatctgctcc ggtgtaccgt ttgccagcgg catcccctga ttgaccacgt
    39901 aaattctgtc tgaaatcccc atcaccagct tcatatcgtg ttcaatcaac aagatagtgg
    39961 tgttgtgatg gttacgcagt tcggcaatca gctcatccag ctctttcgtc tctttcgggt
    40021 taagacctgc tgccggttcg tcgagcatta aaatctccgg ctgcgtcacc atacagcggg
    40081 caatctccag acggcgctgg tcaccatagg ccaggttact cgcctgacgg ttggcatgtt
    40141 ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc gagcgcttcg ctctgggcgc
    40201 gacggaaaga gggggttttc aacaggccgg agaacagccc agttttcagt tgctgatgct
    40261 gtgccaccag caggttttca atcaccgtca tttcacggaa cagacgtaca tgctggaagg
    40321 tgcgcaccac gcccatgcgg gcaatttgct gccccggtaa accttccagg tgctgatcgc
    40381 gcagtaaaat ggtgccgccg gtgggtttat agaatccggt caggcagttg aagaccgtgg
    40441 tttttccggc accgttaggg ccgattaacg agacgatctc ctgcgggtac agttcaagat
    40501 tgacgttgtt caccgccagc aggccgccga agcgcatcat caggccgtta acagataata
    40561 atggctgact catgcctgct ctccttttgc tgcgccgttt ttcagcttga gttgcgggcg
    40621 cgtcatgggc agcaagccct gcggacgcca gatcatcatc agcaccatca aaccaccgag
    40681 cattaacatg ctgtattcgt tgaaatcacg catcaactcg cgcgatacca ccagcaaaac
    40741 agccgcaaga atcactgcaa attgcgagcc cataccgccg agcaccacta tcgccagcac
    40801 aaacgccgat tcggcaaaag tgaaggattc agggctgaca aagccctggc gcgcagcaaa
    40861 caacgttccg gcaaaaccgg caaacgcagc gctgatggta aaggcagtca gcttgatacg
    40921 acgcgggctt aagcccagcg aacgacaagc gatttcatct tcacgcaacg cttcccacgc
    40981 acgccccagc ggcatccgca gcaggcggtt aatgacaaac aggcttaaca ccactagcag
    41041 caacgccacc aggtagagga agatgacgcg atcggaggga tcgtatttca ggccaaagaa
    41101 attgctgaac gtgtcccagc cgccttcgcg ggcggtacgg ctgaactcga gtccgaagag
    41161 cgtcggtttc ggaatctgac tgataccgtt cggtccgccg gtaatttcgg tgttattgag
    41221 cagcaatatg cgcacaattt cgccgaaccc gagggtaacg atcgccagat agtcaccgcg
    41281 cagacgcagc accggaaaac cgagcaggaa gcccgccgcc gccgccatta acccggcaat
    41341 cggcagacag gtccagaagc ccaggccgta atagtgattg agcagcgcaa aagtgtaagc
    41401 gccgatggcg taaaagccgc cgtatcccag caccagcaga ccagaaagac caacaaccac
    41461 gttcagcccg agaccgagaa taatgtagat catggtcaga gtggcgatat ccaccgtccc
    41521 acgcgacacc ataaacggcc acgccactgc aagcaccagc agcgccacga ggaacagttt
    41581 ctgcttcacc gtggagccat caatcgcggg cagaataaac ttcggcccgg aaacgctttt
    41641 tagccctttc tggaaagcag gccgcacaag ctggaagaaa aagaccaccg ccgtgccgat
    41701 aaacacccac tgccaacgga tctctgaagc cgtgtcgacc accagtttgg tgccatccag
    41761 ctccagttgc acgcccataa agacgcccgc cagcacaaag aacatagcgg cagagagcag
    41821 cgccattgca atatgcatcg gtttcatact ttctctacct ccgggcgacc cagaataccg
    41881 gtcggcatca ccagcagcac caggatcagc agggcgaatg agaccacatc tttatattcc
    41941 gtactcagat aggcagaaga gagcgcctcc gcaatcccca ggatcagccc gccaatcatc
    42001 gcccccggaa tgctgccaat cccaccgagc accgcagcgg taaaggcttt catcccggcc
    42061 ataaagccga tgtaggggtt aattacgccg tagaactgac cgagcagcac gcccgccact
    42121 gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca cccggtcggt gttgatgcca
    42181 agcaggctcg ccattttcag atcttccgcg caggcacggc aggcgcgccc catacgggaa
    42241 tagcgaatga aaatcgtcag cgccagcatg gcgaggaaag taacaatcca tatcaccgcc
    42301 tgcatggtgg taatagacgc agagaagttt tcgctatggc ccaccaccca ctgaccgtta
    42361 aacaggctgg gcagcgccac atcgcgcgaa ccttcggtca ggctgacgta gttttgcagg
    42421 aagatagaca tgccgattgc cgagatgagt gcaatcaggc gcttagagtt gcgcaccggg
    42481 cgataggcta cccgttcgat actccagccg taggcgctgg caatgacgat tgcgccgacg
    42541 aatcccgccg ccaccagcag ccagccggta tcaatgccca gcatcatcag tgcggcgatg
    42601 atcataaagg agacgtagct gccaatcatg taaacctcgc cgtgggcgaa gttgatcatg
    42661 ccgataatgc cgtaaaccat ggtgtagccg atggctatca gcgcgtaggt actgcccagc
    42721 gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact gctcagacat aaggtaacct
    42781 ttctaaaccc gcccgttttt cacggacggt gggatgatca cttggctgcc gtggatgaac
    42841 cgtcggcgtg ccactggaag acaccaaaat cgaatccctt aagatcgcct ttttcatccc
    42901 agttcagcgg cccaattacg gtgtttgcac cgttagcttt taaatctttc accagcgcca
    42961 gcggctcatc gctgccggta cgttcaaggg cagttgccag agattgcacc gccgcgtagg
    43021 tgatccagac atagggcccg gacggatctt tcttgtcggc tttcagcgca tcaacgatgc
    43081 cctggtttgt cggatcctgg tcatagcgtt ttggcatggt gaccaacatg ccttcagcgg
    43141 catcaccggc aatgttcgac agtgacgcat taccgacacc ttccggcccc ataaactggg
    43201 ttttcaggcc aacggaacgg gcctggcgca gcatctgccc catttccggg tagtaaccgc
    43261 cgtagtaaac gaagtcgatg ttttcttttt tcaggcgggc gatcagcgcg gagaaatctt
    43321 tctctccagc ggtaatgccg tcgaagaaga cgacgttggc gttagccgct ttcagcccgt
    43381 cctgcaccga acgtgccagc ccttcgccat actgttgttt gtcgtgaatg atggcgatgc
    43441 gctggggctt caccgtctca agaatgtatt ttgccgccgt tggcccctgg gaagagtcca
    43501 gcccggcagt acgcatgatg tattgataac cgcgttgggt cagctccgga ttggtcgctc
    43561 ccggcgagat catcagaata ccttcgtctt catagatatc tgatgcaggt tgggtagagg
    43621 aggaacacaa gtgcccaata acgtatttaa tgccgtcgtt aacgattttg ttggcgaccg
    43681 caacggcttg tttcgggtcg caggcgtcgt catattccac gccaaccagt ttatcgccct
    43741 taattccccc tttggcatta atgtctttaa ttgcctgacg cgcgccgtta aattccatat
    43801 cgccccactg ggcaatcggg ccggacatcg cgccgacaac ggcgacttta atatcgtcag
    43861 ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt ttcgcattcc
    43921 gtttcatagt caaaaatccc cattcgtgat gttgtgttgc tttgttctta tgtgttaaca
    43981 aatcagactg ttctttttta tactgcactg tttttacctg tctgattaag ggtttagcgc
    44041 agtattttgt gatgatagcg attaaaatcc ct
//
LOCUS       DACADA010000021        44023 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.021, whole genome shotgun sequence.
ACCESSION   DACADA010000021 DACADA010000000
VERSION     DACADA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.021"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(92..1375)
                     /locus_tag="ISB17_001139"
     CDS             complement(92..1375)
                     /locus_tag="ISB17_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAP1979094.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1488..3422)
                     /gene="yeaG"
                     /locus_tag="ISB17_001140"
     CDS             complement(1488..3422)
                     /gene="yeaG"
                     /locus_tag="ISB17_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAP1979095.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3858..4604
                     /locus_tag="ISB17_001141"
     CDS             3858..4604
                     /locus_tag="ISB17_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAP1979096.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4694..5548
                     /gene="yeaE"
                     /locus_tag="ISB17_001142"
     CDS             4694..5548
                     /gene="yeaE"
                     /locus_tag="ISB17_001142"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAP1979097.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5599..6483)
                     /locus_tag="ISB17_001143"
     CDS             complement(5599..6483)
                     /locus_tag="ISB17_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAP1979098.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6567..7562)
                     /gene="gapA"
                     /locus_tag="ISB17_001144"
     CDS             complement(6567..7562)
                     /gene="gapA"
                     /locus_tag="ISB17_001144"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP1979099.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7904..8317
                     /gene="msrB"
                     /locus_tag="ISB17_001145"
     CDS             7904..8317
                     /gene="msrB"
                     /locus_tag="ISB17_001145"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP1979100.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8359..8631
                     /locus_tag="ISB17_001146"
     CDS             8359..8631
                     /locus_tag="ISB17_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAP1979101.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9001..10077
                     /locus_tag="ISB17_001147"
     CDS             9001..10077
                     /locus_tag="ISB17_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1979102.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10104..11483
                     /locus_tag="ISB17_001148"
     CDS             10104..11483
                     /locus_tag="ISB17_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979103.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11500..12543
                     /locus_tag="ISB17_001149"
     CDS             11500..12543
                     /locus_tag="ISB17_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAP1979104.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12564..13400
                     /locus_tag="ISB17_001150"
     CDS             12564..13400
                     /locus_tag="ISB17_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP1979105.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            13405..14352
                     /locus_tag="ISB17_001151"
     CDS             13405..14352
                     /locus_tag="ISB17_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAP1979106.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14362..15342
                     /gene="ydjG"
                     /locus_tag="ISB17_001152"
     CDS             14362..15342
                     /gene="ydjG"
                     /locus_tag="ISB17_001152"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAP1979107.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15479..16237
                     /locus_tag="ISB17_001153"
     CDS             15479..16237
                     /locus_tag="ISB17_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1979108.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16355..17713
                     /locus_tag="ISB17_001154"
     CDS             16355..17713
                     /locus_tag="ISB17_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979109.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17806..18447)
                     /gene="pncA"
                     /locus_tag="ISB17_001155"
     CDS             complement(17806..18447)
                     /gene="pncA"
                     /locus_tag="ISB17_001155"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAP1979110.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(18458..19474)
                     /gene="ansA"
                     /locus_tag="ISB17_001156"
     CDS             complement(18458..19474)
                     /gene="ansA"
                     /locus_tag="ISB17_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP1979111.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQKLDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19606..20316)
                     /locus_tag="ISB17_001157"
     CDS             complement(19606..20316)
                     /locus_tag="ISB17_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HAP1979112.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(20432..22288)
                     /gene="sppA"
                     /locus_tag="ISB17_001158"
     CDS             complement(20432..22288)
                     /gene="sppA"
                     /locus_tag="ISB17_001158"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP1979113.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            22449..23000
                     /locus_tag="ISB17_001159"
     CDS             22449..23000
                     /locus_tag="ISB17_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAP1979114.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            23117..24160
                     /gene="selD"
                     /locus_tag="ISB17_001160"
     CDS             23117..24160
                     /gene="selD"
                     /locus_tag="ISB17_001160"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAP1979115.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGVSFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            24165..26120
                     /locus_tag="ISB17_001161"
     CDS             24165..26120
                     /locus_tag="ISB17_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP1979116.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(26197..27540)
                     /gene="gdhA"
                     /locus_tag="ISB17_001162"
     CDS             complement(26197..27540)
                     /gene="gdhA"
                     /locus_tag="ISB17_001162"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP1979117.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            27776..28048
                     /locus_tag="ISB17_001163"
     CDS             27776..28048
                     /locus_tag="ISB17_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAP1979118.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(28014..28421)
                     /gene="nudG"
                     /locus_tag="ISB17_001164"
     CDS             complement(28014..28421)
                     /gene="nudG"
                     /locus_tag="ISB17_001164"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAP1979119.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            28508..29128
                     /locus_tag="ISB17_001165"
     CDS             28508..29128
                     /locus_tag="ISB17_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP1979120.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(29137..30444)
                     /locus_tag="ISB17_001166"
     CDS             complement(29137..30444)
                     /locus_tag="ISB17_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAP1979121.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(30511..31164)
                     /locus_tag="ISB17_001167"
     CDS             complement(30511..31164)
                     /locus_tag="ISB17_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP1979122.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(31164..32699)
                     /locus_tag="ISB17_001168"
     CDS             complement(31164..32699)
                     /locus_tag="ISB17_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1979123.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVVKWVGYVRQ
                     GLR"
     gene            complement(32672..33838)
                     /locus_tag="ISB17_001169"
     CDS             complement(32672..33838)
                     /locus_tag="ISB17_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1979124.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(33848..34396)
                     /locus_tag="ISB17_001170"
     CDS             complement(33848..34396)
                     /locus_tag="ISB17_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP1979125.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(34396..35103)
                     /locus_tag="ISB17_001171"
     CDS             complement(34396..35103)
                     /locus_tag="ISB17_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP1979126.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(35117..35794)
                     /gene="ydjY"
                     /locus_tag="ISB17_001172"
     CDS             complement(35117..35794)
                     /gene="ydjY"
                     /locus_tag="ISB17_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAP1979127.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(35799..36509)
                     /locus_tag="ISB17_001173"
     CDS             complement(35799..36509)
                     /locus_tag="ISB17_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP1979128.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(36676..37482)
                     /gene="xthA"
                     /locus_tag="ISB17_001174"
     CDS             complement(36676..37482)
                     /gene="xthA"
                     /locus_tag="ISB17_001174"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP1979129.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            37928..39148
                     /gene="astC"
                     /locus_tag="ISB17_001175"
     CDS             37928..39148
                     /gene="astC"
                     /locus_tag="ISB17_001175"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAP1979130.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
     gene            39145..40179
                     /gene="astA"
                     /locus_tag="ISB17_001176"
     CDS             39145..40179
                     /gene="astA"
                     /locus_tag="ISB17_001176"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAP1979131.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            40176..41654
                     /gene="astD"
                     /locus_tag="ISB17_001177"
     CDS             40176..41654
                     /gene="astD"
                     /locus_tag="ISB17_001177"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAP1979132.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            41651..42994
                     /gene="astB"
                     /locus_tag="ISB17_001178"
     CDS             41651..42994
                     /gene="astB"
                     /locus_tag="ISB17_001178"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAP1979133.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            42987..43955
                     /gene="astE"
                     /locus_tag="ISB17_001179"
     CDS             42987..43955
                     /gene="astE"
                     /locus_tag="ISB17_001179"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAP1979134.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT        10756 a        10743 c        11580 g        10944 t
ORIGIN      
        1 ttaaagcgat tgtaagctaa tgtatgtaat aaaagagata atttacagtg agctaaaaat
       61 cagccaggcg ataatgaccc ggctgacagt tttagtcttt agctgttgca ttttgtttat
      121 gaaacagttc acggaacacc ggataaatat catcctggtc gcggatatgc tgcatcgcaa
      181 agttgtcgaa agtagattgc agatgctcat attctcgcca cagtgtctga tgcgcgcgac
      241 gtgtaatttc gatatagctg taataacgaa caacgggtaa tatttttttc gccagaattt
      301 catggcaaag cggagagtca tcggcccagt tatcgccgtc tgatgcttgg gctgcgtaaa
      361 tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt tttagggcgc
      421 tggaaacaat ggtgccgcca gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt
      481 tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt ctgctgagga
      541 acagatacag cagaatataa aaacgtttag ccatatcttt agtggactga tccattgaac
      601 cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc cgtttttcgt
      661 agttcttgta acgtaaatcg aaggtgtcaa tgaaagggac gcgttcaatt ttggcacgta
      721 attctgcaat ttctttgcgc agacgttcct cttccagcag ttgcgcaggt tcactgttgc
      781 tgatgatggc caaattctct tccagcgcat gaagttcccg ccgcttgccc gccgtcatag
      841 ctgtgcgccg cgccagtgag ttctgcaatg aacgtacaac gctgatattg gccggaacgc
      901 cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt tgttggtttt
      961 gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca tctttcgaaa
     1021 tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc tgaccactgc
     1081 cggaacctcc gccaccaccc tggggacgtt caattcggtc gttctggacg aaatggtcat
     1141 tgcccggatg cacacggtgg cgcaggcccc cacgcccctg atgaaacatc ggttcgctaa
     1201 tatcttccgt gggaatggat acggactcgc cgctgtcgac gtcagtcacc gaacgcttat
     1261 taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaag aaacgctggc
     1321 gattcaccat gcttttattt ttgccgttca gacgccggtc aataaaccag gtcatatgcc
     1381 ccccgtacta catttgccaa cggtatgcta cgcttgtgtt gccgcttctc tggcccacgg
     1441 ttcgctgtcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta agacgattta
     1501 cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc
     1561 atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta
     1621 aacgaaataa ccggcaacag ctcttcggta ttggagaaca ttttcttctc gataaccgtg
     1681 cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc tctggctcgc
     1741 agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc cgccggtttc
     1801 tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc ggtatccgga
     1861 tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg gtcgaaaatg
     1921 ttttgcccat attctgaata ggattcaagg taggccgtct ggatctcttt gccgataaac
     1981 tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc agcctgctct
     2041 tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg gaccgggttt
     2101 gccgccactt ctacatgatc gaagttgaac acgcgggaga ggatcttaaa cgcaaaacgc
     2161 gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc ctgatacgac
     2221 ttggctttgg gatcagtgtc tttcagactt tcaccatcat aaacgcgcat ctttgaataa
     2281 atgctggagt tttctggctc tttcagacgc gaaagaatag aaaaacgtga cagcgtttcc
     2341 agcgtgccag gggcgcaggg ggcgtgagtc aattcactgt gattaagcaa tttctcgtag
     2401 attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta aacacggtcg
     2461 aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggattc gttcgagtgg
     2521 gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt gtagttacct
     2581 tcctgggtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa catctcgaca
     2641 aattccatga tcccctgatt ggcgcggcac agcgcaccgg aatagccgta ggcgtccggg
     2701 tcattctgcg cgtagtgttc gagtttacga atatcgactt tccctaccag ggcggagatg
     2761 tcctggttgt tctcatctcc gggttccgtt ttggcgatgg caatttgttg cagaattgac
     2821 ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg caggcgcttt
     2881 gccgcccacg gcgacatgat ggtgccgaga taacggcgag gaatgccgta ctctttttcc
     2941 agaatctgcg catcttcctg cggattgaaa agacagaacg gatgatcgtt gaccgggcta
     3001 cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga tttcagtcgc
     3061 tcagcaagtg atgatttacc cccacccaca ggccccagca gatacagaat ttgtttcttc
     3121 tcttccagcc cctgagccgc gtgtttcaga taagaaacaa tctgttcaat cgcgtcttcc
     3181 atgccgtaaa actcttcaaa cgccggataa cgtgcaatga cccggttaga aaagagtcga
     3241 gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccaatagc catcaatagc
     3301 cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag aaactcctgc
     3361 agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc gaatatattc
     3421 atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat gaatagtaga
     3481 ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc
     3541 attttattga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg
     3601 gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca acaactcatc
     3661 cataaatttt gcataattaa tgtaaataat agggtcgccg gtaacgctaa attcatttgg
     3721 ctgtaagcgt ggtgtcatct gcgtcaggaa aattaaacag ttactataaa aaatgaaaac
     3781 gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt
     3841 ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg cttatcgcaa
     3901 cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc gtaggtgtcg
     3961 ttgaacaccc atataaagat tacgataccg atgtttaccc agtaccggta atcaactatg
     4021 aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg aatgacgcga
     4081 cggacaaact ttcaattacc gcttactggt cgccgcttta cttcaaagct aaagacagtg
     4141 gcgatcacca aatgcgtcac ctcgatgacc gtaagagcac catgatggct ggtctgtctt
     4201 atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat accctggata
     4261 acagcaacgg catcgtctgg gatatggcct ggttgtatcg ttacaccaac ggtggcctga
     4321 ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa tactattatg
     4381 gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg ctataacccg aacgacagct
     4441 ggagccctta cctggagctg agcgccagct acaacttcct cggcgactgg agtgtttacg
     4501 gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccaatg gtggataaat
     4561 cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc atctttccag
     4621 ggcattttgt gcataaaaac agggcgtaat gccctgtttt gcattacggt ggtgcaatac
     4681 ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct cactgccagc
     4741 catagggcag ggaacatggt atatgggcga agatgccagt cagcgtaaaa cagaagttgc
     4801 tgcactacgc gcgggcattg aactcggttt aaccctcatt gataccgccg aaatgtatgc
     4861 cgatggcggt gccgaaaaag tggttgggga agcattaatc ggtctgcgtg agaacgtctt
     4921 tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga
     4981 agccagttta cgccgtctca atactgatta tctcgatctc tacttattac actggtctgg
     5041 cagtttcgct ctcgaagaga ctgtcgcagc gatggaaaaa ttgatcgccc agggaaaaat
     5101 ccgccgctgg ggcgtttcta accttgatta tgccgatatg caggaactct ggcagctgcc
     5161 ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac gagggattga
     5221 gtacgatcta cttccctggt gccagaaaca gcagatgccg gtgatggctt acagtccatt
     5281 agcccaggcc gggcggttgc gcaatggact gttaaaaaat gcggtagtca acgaaattgc
     5341 acatgctcac aatatcagtg cggcacaagt attgttggcg tgggtaatca gccatcaggg
     5401 tgtgatggcg attccaaaag cggcaactgt cgcacatgtc caacaaaatg cggctgtgct
     5461 tgaggttgaa ctttcgtcag cggaattagt tatgctggat aaagcgtatc cggcaccaaa
     5521 aggaaaaact gcgctggata tggtgtgatg ttgattcgtg ccggatgcat cgcacccggc
     5581 acacaatatt aacgcaaatt aacgtttagc aacgcgaatg gattgcgcca gatgtgcagg
     5641 tttctctttg gtgacttttt gcgtttctga agcgtaagcc gtttctacac aaacaaaggt
     5701 cttgtagcca tcatccggca tatcgcccat gctaactgaa agcgccggtc ctgggttcca
     5761 gcctacaacg ttcagatgat gctggtggcc tacggcgata atacgattca gcgcttcgtc
     5821 attaattacg ctgcaatctt gcggattcag atacacgcga tcggtacggt cagggaaggt
     5881 ctgaataccg tcggtcagta catcttcttt cgcgtcattt actttatcaa tgaagcgatc
     5941 gcctagtcca ctgacgctta ccttagcgat atcacccacg ttaaagtagg tatgcagagc
     6001 agaggtagtt tcaaattcgc cgtgtgcttc aagatcgatt tcacaggttt tacccacgtg
     6061 gaaatgcgct aacagcgtaa agtcgtgcgg ccagaatttt ttcgtctctt cgctttgtgt
     6121 caattcaaaa gtcagcgcta caccatcagc atcttcacga tgtgatttca gcgtccacgg
     6181 caggttgcgg gcaaaaccgt gcgcaggcag accttgttgt gcagccggac caaaccacgg
     6241 ccagcaaacc ggtatgccac cgcgaatggc gacgccattt ttgaacggtg tgttgttact
     6301 caaccacaga acttcttctt cacccgcagg tttccacgag agaaggtgtg cgccctgtaa
     6361 tgcaaaagag gcttttacct ggggatgatc gaccacaatg aggtccagtt catccagttt
     6421 acgacgggag aggacagggg agatttgttc gatgaccgga agggcaaaaa ttttcttaat
     6481 catgacgcag tcctttaact tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc
     6541 tttttagatc acagtgtcat ctcaacttat ttggagatgt gagcgatcag gtccagaact
     6601 ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc
     6661 agagcgatac ccgctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta
     6721 gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcagct
     6781 ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca
     6841 actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt
     6901 tctggcagta ctttacctac agctttagca gcaccggtag aagacgggat gatgttctgg
     6961 gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta
     7021 gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata
     7081 actttagcca gcggagccag gcagttggtg gtgcaggatg cgttggaaac gatgtcctgg
     7141 ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga
     7201 ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc
     7261 aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta
     7321 gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct
     7381 ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc
     7441 atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga
     7501 gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc
     7561 atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct
     7621 tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca
     7681 ttacgcgact gactcgcctc actcttcctt tgggcttgag accacatggg gtcggcgccc
     7741 cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca
     7801 tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa
     7861 tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc
     7921 ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttc tacgtgacgc agaatcatgg
     7981 gacagaaccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg tatatcactg
     8041 tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg gctgtggctg
     8101 gcccagtttc tacgaaccgg tgagtgaaga atctattcgt tatatcaaag acttgtcaca
     8161 tggaatgcag cgcatagaaa ttcgttgcgg taactgcgat gcccatctgg gccatgtctt
     8221 tcctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt ctttacgctt
     8281 cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc
     8341 cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac gcctgaggta
     8401 taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tacgttgacc
     8461 gaggaacaaa aagagaactg cctgcaactg gtgatgctgt ggcaagcccg ccataatacc
     8521 gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag
     8581 ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta agtggtttat
     8641 cccctttttg ccgcggttat aaaggggata gccaacaatg accccacgac tcatctcaat
     8701 tatctcgccc cgacctggct aagtcccagt ccgatctctt gcagtcgcgt tcacacctga
     8761 atgacttctc ctgaacttaa gtctcaggcc tatttggccg ggaatccctc tcgaatttgc
     8821 tctttttgcg tcccgccttc catcaacctg taacaacccc agccattttc tgcgtaattg
     8881 tgatctaagt tgtaaaacgc agatcattat ctgtgtttta cagatttaat gttgacctca
     8941 aaaatcatgt gggtaagtat gatgaccatc taagcaaaac aaaacacaga ggatgacgca
     9001 atgaaagcac tggctcggtt tggcaaggct tttggtggct acaagatgat tgatgtccca
     9061 caacccattt gtggcccgga agatgtcgtg attgaaatta aagccgcggc aatctgcggc
     9121 gcagacatga agcactacaa tgtcgatagc ggttcggatg agtttaactc tatccgcggc
     9181 catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg
     9241 caacgcgtcg tgtcggataa cagcggtcac gtttgcggcg tttgcccggc ctgtgaacaa
     9301 ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa tacctggggc
     9361 ggtggttttt ccaaatattg tcttgttcct ggtgaaattc tcaaaattca tcgtcatgcg
     9421 ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga ccctatctgt
     9481 aatgcctata aatccatcgc gcagcaatcg aaattccttc caggtcagga tgtggtcgtc
     9541 atcggcactg gcccactcgg gctgttctcc gtacaaatgg cgcggattat gggggcggta
     9601 aatatcgtcg tcgttggtct gcaagaagat gtaacggtcc gcttcccggt tgcaaaagaa
     9661 ctgggtgcga cggcagtagt aaatggttct accgaagatg tggtggcacg ctgccagcaa
     9721 atttgtggca aagacaatct ggggctggtg attgaatgct ccggtgccaa tatcgcattg
     9781 aaacaagcca tcgaaatgct ccgcccgaat ggggaagtag tacgcgttgg aatgggcttc
     9841 aaacctcttg atttctcgat taatgacatt accgcctgga acaaaagcat cattgggcat
     9901 atggcctatg actccacttc atggcgtaac gctatcaggc tattagccag cggcgctatc
     9961 aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat
    10021 gcgatggtcg ataaaactgc aatcaaagtg atcatgactt acgactttga tgaataacta
    10081 ctctcctgaa ggaacgttat cgcatggaac agataacaaa accgcattgt ggtgcacggc
    10141 tggatcgctt accggattgc cgctggcatt cgtcgatgtt tgccatcgtc gcgtttggcc
    10201 tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg
    10261 gctggacaga taattccacc actgccacat tctcagcaat cacgaccgcc ggaatgtttc
    10321 tcggtgcgct ggtgggcggc atcatcggtg acaaaaccgg tcgcagaaat gcgtttatcc
    10381 tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt
    10441 tccttattgc ctgccgtttt gtgatgggcg ttgggctggg agctttactg gttacactgt
    10501 ttgctggttt caccgaatat atgcccggaa gaaatcgcgg aacgtggtcc agtcgggttt
    10561 cctttattgg taactggtca tatccgctct gttcattgat tgcgatggga ctcacgccgc
    10621 tgattagtgc agagtggaac tggcgggtac aactgcttat ccctgcaata ttgtcactta
    10681 tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg
    10741 gacgttatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc atacgccaga
    10801 cgggtaaacc tttgccgcct gtggttattg ctgatgacgg taaagcacca caagcggtgc
    10861 cgtattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc
    10921 tgattgccat gaacgttgtg cagtacacac taattaactg gttgccaacg atattcatga
    10981 cccagggaat taatttaaaa gactcgattg ttttaaatac catgagtatg tttggcgcgc
    11041 catttggtat ttttattgcc atgctggtga tggataaaat tccgcgtaaa acaatgggtg
    11101 tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa accagtatgt
    11161 tgctcataac gctgattggt ttcttcctga ttactttcgt ctatatgtac gtttgctatg
    11221 cctcggcagt gtatgtccct gagatctggc cgacagaggc caaactccgt ggctccggtc
    11281 tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc
    11341 tcagtagtta tggcgtaacg ggagtcttta ttcttctggg ggctgtttca attattgtcg
    11401 caattgctat cgccaccatt ggaattgaaa ccaaaggtgt ctccgttgaa agtttaagta
    11461 ttgatgcagt agccaataaa taatactggt ggattcaaaa tgaaaaattc aaaagcaatg
    11521 ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa tacctgtacc taaagaagat
    11581 gaagttttga ttaaagtgga atatgtcggt atttgtggtt cagatgtaca tggttttgaa
    11641 tcaggcccgt ttattcctcc taaagaccca aatcaagaaa ttggcctggg tcatgaatgc
    11701 gccgggacgg ttgtggctgt gggaagccgc gtgcgcaaat ttaaaccggg ggatcgggta
    11761 aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg caaatataac
    11821 atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc attaacgcac
    11881 tatctgtgtc atccggagag ctttacttac aaactgccag acaatatgga cacgatggaa
    11941 ggggcgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc agatgttaaa
    12001 ccgggtaaga agataattat tctgggagcg ggttgtattg gtttgatgac gttgcaagcg
    12061 tgcaaatgcc tgggagcaac ggaaattgcc gtcgttgatg tgctggaaaa acgtctgaca
    12121 atggcggaac agctcggcgc gacagtggtt attaacggcg caaaagaaga cactattgca
    12181 cgctgtcagc aatttaccga agacatgggc gcagatattg tttttgaaac agcgggttct
    12241 gcggtcaccg ttaaacaggc accttatctg gtcatgcgcg gcggtaaaat tatgattgtt
    12301 ggtactgtac ccggcgattc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc
    12361 cagacggtat tccgctatgc caatcgttat ccggtcacga ttgaagctat ttcttcaggg
    12421 cgattcgatg tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca
    12481 tttgaagagt cagttaacaa caaacgcgac attattaaag gcgttattaa aattagcgat
    12541 taattaatac gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca
    12601 caaataaaca ttatgcaatt gcccatttta acgtatggaa tgcagaaatg ttgatgggcg
    12661 ttatcgacgc agccgaagaa gcgaaatcac cggttattat ttcttttggc acaggttttg
    12721 tcggcaacac ctcatttgaa gatttctctc acatgatggt atcaatggca caaaaagcaa
    12781 cggtgccggt aataactcat tgggatcatg gtcggagtat ggagattatt cataacgcct
    12841 ggactcatgg tatgaattca ttaatgcgtg atgcttctgc atttgatttc gaagaaaata
    12901 ttcgtttaac caaagaggct gttgatttct tccatccgct gggtattccg gtagaggcgg
    12961 aattagggca tgtcggtaat gaaaccgttt atgaggaagc gctggcaggc tatcactata
    13021 ccgatcctga ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg
    13081 ccatcggcaa ccagcatggg gtttatacgt cagagccaca attgaacttt gaggtcgtca
    13141 aacgcgtacg cgatgctgtt tctgttccgc tggttttgca tggcgcatca gggatcagtg
    13201 atgccgacat taaaactgca atttcgttgg gtattgcgaa aatcaacatt catacggagc
    13261 tctgtcaggc cgcaatggta gcagtgaaag aaaaccagga tcagcctttc ctgcatttag
    13321 aacgtgaagt gcgtaaagct ataaaagaac gggcattgga taaaattaaa ctgttcggtt
    13381 ctgatggcaa agcggaataa taatatggat aatctcgacg ttatttgtat aggtgccgct
    13441 attgttgata ttccattgca accggtcagt aaaaatatct ttgatgtgga ttcttatcct
    13501 cttgaaagaa tcgcaatgac caccgggggt gatgcaataa atgaagcaac aattatttct
    13561 cgtctgggcc atcgcacagc attaatgagt cgtattggta aagatgccgc agggcaattt
    13621 attctcgacc attgccgcaa agagaatatt gatattcaaa gcctgaagca ggacgtcagc
    13681 atagatacct ctattaacgt tggactggtg acggaggatg gcgagcggac ctttgtcaca
    13741 aaccgtaacg gcagtctgtg gaaattgaat attgacgatg ttgattttgc gcggttttct
    13801 caggcgaaat tattatcact ggccagtatt ttcaacagtc ctctattgga tggtcgagcg
    13861 ctaacagaaa tttttacgca agctaaaacc cggcagatga ttatctgtgc cgatatgatc
    13921 aaaccgcggt tgaatgaaac gctgaatgat atttgcgaag cattgagtta tgtcgattat
    13981 ctgtttccta attttgccga ggcaaaatta ctcaccggga aagagacact ggatgaaatt
    14041 gctgactgct ttcttgcgtg cggcgtaaaa acggtggtga ttaaaacggg taaagacggc
    14101 tgctttatca agcgtggtga catgacgatg aaggtgccgg cggtcgcagg aataaccgct
    14161 atcgacacca ttggtgcggg cgataacttt gcttcaggtt ttattgcagc actgttagaa
    14221 ggcaaaaatc tgcgagaatg cgcacgcttt gccaatgcaa cggcggctat ctcggttcta
    14281 agcatcggcg ccaccaccgg tgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa
    14341 tacgaaggat aatgaaggca aatgaaaaag atacctttag gcacagcgga tataacgctt
    14401 tcgcgaatgg ggttgggtac atgggctatt ggcggcggtc ctgcatggaa tggcgatctc
    14461 gatcgacaaa tatgcattga tacgattctt gaagcccatc gttgcggcat taacctgatt
    14521 gatactgcgc caggatataa ctttggcaat agtgaagtta tcgttggtca ggcgttaaaa
    14581 aaactgcccc gtgaacaggt tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa
    14641 ggaagtttat tcaacaaagt tggcgatcgg cagttgtata aaaacctttc tccggaatct
    14701 atccgcgaag aggtagaagc cagcttgcaa cgtctgggta ttgattacat cgatatctac
    14761 atgacgcact ggcagtcggt gccgccattt tttacgccga tagctgaaac tgttgcagtg
    14821 cttaatgcgt taaaggccga agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct
    14881 gaccatatcc gcgagtatct gcaacatggt gaactggata ttattcaggc gaaatacagt
    14941 attctcgacc gggcgatgga aaacgaactg ctgccgctat gtcgtgataa tggcattgtg
    15001 gttcaggttt attccccgct agagcaggga ttgttgaccg gcaccatcac gcgtgattac
    15061 gttccgggcg gcgctcgggc aaataaagtc tggttccagc gtgaaaacat gctgaaagtg
    15121 attgatatgc ttgaacagtg gcagccactc tgtgctcgtt atcagtgcac aattcccact
    15181 ctggcactgg cgtggatatt aaaacagagt gatttaatct ccattcttag tggggctact
    15241 gcgccggaac aggtacgtga aaatgtcgcg gcactgaata tcaacttatc ggatgcagac
    15301 gcaacattga tgagggaaat ggcagaggcc ctggagcgtt aaatactcaa cacgattcac
    15361 ctctgtttct gtgaaaactg tgatgaaaat ctgtgattca aacaggttat tttgaaagta
    15421 aacatcggtt atgcgataat tgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt
    15481 ggcggcaaaa gacaggattc aggcaattaa gcaaatggtt gccaacgata aaaaggtgac
    15541 agtctcaaat ttgagtggga tttttcaggt aaccgaagaa accattcgcc gcgatcttga
    15601 gaagttggaa gatgaaggct ttttgaccag aacctatggt ggtgctgttt taaatacagc
    15661 gatgttgacg gagaatatcc atttttataa gcgcgcttca tcgttttatg aagagaagca
    15721 gcttattgca cgcaaggcac taccctttgt cgacaataaa accaccatgg cagccgattc
    15781 cagtagtacc gtaatggaat tgctcaaatt gttacaggac cgtagtgacc tgacgttgct
    15841 aaccaactcg gcggaagcga ttcatgttct ggctcagtca gaaattaaag tcgtttcaac
    15901 gggtggggaa ctaaacaaaa atacactttc actgcaagga agaattacta aagagatcat
    15961 cagccgctac catgtcgata tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg
    16021 cgcgctggac tctaatgaag cggaagctga aatcaaaaag acaatgatcc gtcaggcgac
    16081 agaagttgcg ttattggttg atcactctaa gtttgatcgc aaagcttttg tccagttagc
    16141 tgactttagt catattaatt acataataac tgataaatca ccgggtgcag agtggatagc
    16201 attttgcaaa gacaataata tccagctggt gtggtaattt aagcacacat tgtcaatatt
    16261 atttgttcct gcgcctggaa ataaaatgat gataaagatg cggcaagtga atttgccgca
    16321 attgctacct tttttagcaa atccgaggca cccaatggaa caatatgatc aaattggcgc
    16381 aagactggac cgcttgcctt tggcccggtt tcattatcgt atatttggta taataagctt
    16441 tagtctgtta ttaacggggt ttttgagtta ctccggtaat gtcgtcttag caaagctggt
    16501 aagcaatgga tggtcaaata atttcctcaa tgccgccttt acctcggcat taatgtttgg
    16561 ttatttcatc ggctctctta ccggtgggtt tattggtgac tactttgggc ggcgcagggc
    16621 gtttcgcata aatcttctca tcgtcggtat tgcggcaaca ggggccgctt ttgtccctga
    16681 tatgtactgg ctcatcttct ttcgcttcct gatgggaaca ggaatggggg cgctgattat
    16741 ggttggctat gcctcattta cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc
    16801 gcggctctca tttgttggaa actggtcgcc catgctgtct gcggcgatag gcgtggtggt
    16861 tatcgctttt tttagttggc ggataatgtt tctgctgggc ggtattggca tactgttagc
    16921 ctggtttctc tcaggtaaat actttattga gtcgccacga tggttggcag ggaaagggcg
    16981 aatcgcaggt gcagaaagcc aacttcgtga agtagagcag caaattgaaa gagagaagag
    17041 tattcgttta cccccgctta ctttgaacca gagcaacagc aaggttaagg taatcaaggg
    17101 taccttctgg ctcctgttta aaggggaaat gttacgacgt acattagtcg cgattactgt
    17161 tttaattgca atgaacattt cgctttatac catcaccgta tggatgccga ccatatttgt
    17221 taactccggc attgatgtcg ataaatcaat attaatgacc gctgttatta tgattggcgc
    17281 tccggtaggg atatttattg cggcattaat tattgatcat ttccctcgtc gattatttgg
    17341 ttccgcctta cttattatta ttgccgtgtt aggctatatc tattcaattc agacaacaga
    17401 gtgggcgatt ttaatctatg gcctggtgat gatcttcttt ttatacatgt atgtttgctt
    17461 cgcgtcggcg gtttatatcc cggaactttg gccaacgcat ttacgcctgc gtggttcggg
    17521 tttcgttaat gccgtaggac ggatcgtcgc agtcttcacg ccctatggcg ttgcggcatt
    17581 attaacacat tatgggtcga tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg
    17641 tgcgctggtt ctctccattt ttggcatcga aacgcggaag gtgtcgctgg aagagatttc
    17701 tgaggtgaat taaatctttc gttgatggcg tcaattacca gatgtatcac tgatgtgttt
    17761 tatctggcct acaatgccag tacagaattg taggccagcg taaaattact cctgcgtttc
    17821 ttcccagtct gccagcgtat atagcgttgc cccagctgct gacatttcca taaacgcgtg
    17881 cgcactgtcc tgtggctgga tatttacgcc acgacaacca tcggtaatca cgttcacctt
    17941 atagcctaac tgtaaagcgt ccagcacggt aaacttaacg caatagtcag tagccagacc
    18001 cataacgatc aattcattga ttacatgagc gcgcaaccag tcatcgagcg cagttttctg
    18061 ccgacgaccg ttatcaaaaa aggcactgta actgtcaact aaaggatttt cgcctttatg
    18121 gaacaccgct gcgatcgctt tttgcttcag taacggatgt aattgtgcgc cttcactgtt
    18181 ctgcacacag tgatcgggcc agaaggtttg tggcaaaccg tcgagttggc ctggcgtata
    18241 aggctctaca ccgtgctgac tggcaaaact gccgtgattc gccggatgcc agtcctgact
    18301 ggcgataact gcttcaccgc gcgactggca ccagtcaatc aggcggttag cgacatccac
    18361 cgtactgtca ccttccggca cggcgagcgc gccaccagca cagaaatcat tttgtaaatc
    18421 gaccagtaac agggcgcgag ggggcattgc agtctcctta atcatccggc gtcagttcgc
    18481 cacgcaggtt ttggctcatg gccttgcgaa tggtttcagt atcaagtttc tggctaagca
    18541 ggtaatgcag tttggttagc gtggcttcta cagtcatatc tgcaccgcca ataacgccgg
    18601 catgggcgag ggcgttaccg gtggcataac cacccatgtt cactttaccg gacatacatt
    18661 gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg
    18721 ctttgttttg tggcgcatta cccacgccat atgagcgcag aatcaatgct ttcacaggtt
    18781 ggcgcagaaa attgcgcacg acgtcagcag aaatccccgg ataaatcgtc actacgccaa
    18841 ttggttgcgg cgtgattgga tgaacgatca attccccttc gccgtgcggg gcgggtggcg
    18901 tattcaaacg acgaatatgg atacctgctt ccagtaacgg aggaaggttt ggagaggcaa
    18961 acgcatcaaa accatcggca tgggctttgg tggttcggtt gccacgatac agtcgattat
    19021 taaaaaagag agttacttcg ttgataggat aattcgccgc aacgtacaac gcattcagca
    19081 gattaatttg tccgtcagag cgtaactcag ccagcgggat ttgtgaccct gtcacaatga
    19141 ccggtttact gagattctcg agcatgaacg acagtgcaga ggcggtatac gccatcgtgt
    19201 ctgtgccgtg cagaatgaca aaaccatcat agtcgtcata gtgcgcttta atatcttcag
    19261 caatatgctg ccagtcttct ggcgtcatat cagaagaatc catcaatggc gtatattcat
    19321 gaatggtgaa atctggcatc tccgggcgat ggaattccgg catcagcgcc agttggcgct
    19381 gtagatgacc tgacaccgga atataaccct gctcggaacg ctgcatcccg atggtcccgc
    19441 ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt gatatgaatg atatcgaaag
    19501 agcaaaagta tatacgtgag gggcagcgag cgcacctgga tactctgtgg atatgagaaa
    19561 agtccggcag aacggaggga ttagtgtccc gccggagaaa agtggttaat cctgcaacgc
    19621 ataggttacg gtggtgacta cgcgtaccgt tttattaatc gccgtaggat catcttcatc
    19681 actgccactg gcgaagatct ggaacacgcc ttgtgaggca tcgctaattg accccacctg
    19741 gctacctgag tccgttgcaa attgtaatgc tgcgttgcgt gcatctttgg tggcggcggc
    19801 aatcatctca gcgcgcagtg cgccaatacc attaaagcga taggcgactt tgctctcagc
    19861 gatagcgccc tccgtagcga cttgcagttc gtttaatttc gcgacgactt tttcgacagc
    19921 agcaacatta tgggttttta tcgtcactga cccgtctgcg ttatagcgtg gcattgtggg
    19981 atcatctttc agataaaagg aatctgttcg cttgcttgtc cattggccca gggttatctc
    20041 tttctcgtta atcccctgag cattgagttc ggcaacaatt tttttctgcg cgtcttccag
    20101 tttcgggaac agttcgccgg gaatattacc cttaaagtta atcgcaattg ttaattctgc
    20161 cgtatctgcg cgaacttctt tttcagaaag cccacgcaca tttacataac ggttattggt
    20221 ttttaaatgt ttaacaccat cgccaacaaa ataaccacag gcgctaatcc ccagtccgag
    20281 gacgatagcg gcaattaaag agggggcctt attcactgat gactccatat cagatgctaa
    20341 atgagcaagg gaatgtagtg gagagaggga gtgaaagaca gtccgattca ggagtgtggc
    20401 cgacagcata tcggccacat ttttgcagga cttaacgcac gttggcgcag gtcaggcaaa
    20461 acgcataacg gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg
    20521 tagaggctac cgaggccagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg
    20581 cccggacaga accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt
    20641 agtattccag atgccactgt ttcacttttg ccagctctgc tgctttggcg accgcatcat
    20701 cgaaatcccc gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt
    20761 ggccctgggc gattttatca atctgctccg gcgttgaatg acgcgcatca gcaaccagcg
    20821 tgataaagcg tttatagcca ttctcaatgc ttaactgcat catctgctgc acttccggcg
    20881 gcagtgccct ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa
    20941 caccaatcga atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac
    21001 cggtcagggt gctggggtta gccacaatgt aattagctgg tgtggaaatc cagtaaccac
    21061 cagatgccgc catgccaccc atcgatacaa ccacaggctt acccgctgcc cgggctgctg
    21121 ccagttcagc gcgaatcact tcagacgcgg taacgctgcc gccgggacta ttaacacgca
    21181 gaacaatcgc tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat
    21241 caccgccaac attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga
    21301 caccgatact gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg
    21361 cgcgataatt tttatcagtt ttactccagc cgaactcttt ggtcagagtt ttttcgattt
    21421 cggcactcga tgccagtgca tcgaccagct tgttttccag cgcatatttc gcggtatcgc
    21481 caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag
    21541 ggatctggcg gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc
    21601 ggctgtcagc ttcgcgggct gccggcgaca tatcatcacg aataaacggt tcaacggcag
    21661 atttatacgt acccacgcgg aacacatggg tggaaacttt cagcttatcc agtaacgatt
    21721 tgtagtacag accgttggtg gcaaagccgt gcagatcaac cacgccttgc ggagacagcc
    21781 aaattttatt ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg
    21841 cataaaccgg tttcccgctg tcacgaaact ctttcagagc tttgccgatg tactgcatag
    21901 acggctggtc gccgcctgcg aagtttttca gatccatcac aataccggtg atattgcggt
    21961 cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgaagacgat
    22021 cggaactggc accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtcgggtt
    22081 tatcgacgat cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg
    22141 aatcaccacc actgacctgc atccagatcc ccacaccaac cagcacgaga aaaataaaga
    22201 acaggttaag taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc
    22261 cggcaataaa tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtttc
    22321 aggcgcaata gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg
    22381 actgggctgt aacaaaatcc actctcgtgt taattttgtg aataaatatc atgacaggag
    22441 ttaatcaaat ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg
    22501 aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg
    22561 accataagtc catgcaaccg tggcatttct ttgtgattga aggggaaggg cgcgagcgtt
    22621 tcagcgccgt actggagcag ggggcgattg ctgccggtag tgataacaaa gcaatcgaca
    22681 aagcgcgtaa tgcgccgttc cgcgcaccgc tcatcataac ggtggtggcg aaatgtgaag
    22741 aaaatcataa agtcccgcgc tgggaacagg aaatgtctgc gggatgcgcg gtcatggcga
    22801 tgcaaatggc agcagttgcc caggggtttg gcggcatctg gcgcagtggt gcattaactg
    22861 aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc
    22921 tctacctcgg tacgccgcag ttgaaagcat ctacgtcgat aaacgtcccg gacccgacgc
    22981 cgtttgtgac ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca
    23041 ggaattcaga cactctcact tatcacttca tggaatgagg gttaccatac ccggattgca
    23101 ttgacaggag atgtccatga gcgagaactc gattcgtttg acccaataca gccacggcgc
    23161 tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc
    23221 gaagtttgtt gatccgaatt tgcttgtggg taatgaaacc cgcgacgatg cggcggtgta
    23281 cgatctgggc aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga
    23341 taatcctttc gattttggcc gcattgcggc gactaacgcc atcagcgata tctttgctat
    23401 gggcggcaaa ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc
    23461 ggaaattgcc cgcgaagtga ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc
    23521 gctggctggc ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg
    23581 gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt
    23641 cctgacgaaa ccgctgggga ttggcgttct taccacggct gagaaaaaat cactgctgaa
    23701 accagaacat cagggactgg cgacggaagt gatgtgccgg atgaacatcg caggcgtgtc
    23761 ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt acgggttttg gtttactggg
    23821 gcatttaagc gaaatgtgtc agggcgcagg tgtgcaggcg cgcgtcgact atgacgcgat
    23881 cccgaaattg ccgggggttg aagagtacat taagttgggc gcagtgcctg gcggcaccga
    23941 acgtaacttt gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgatct
    24001 gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa
    24061 tgaggtcaaa gctacagccg ccgagtttgg cattgaactg acggcgattg gcgaactggt
    24121 gccagcgcgc ggcggtcgtg ccatggttga gatccgttaa ttcaatgcgg ttgtttattg
    24181 ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct gcctaaaccg caccggaaag
    24241 gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc
    24301 ttgagcaggc gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt
    24361 tgccaattgt cccggaaaag tggcaattac agccccgacc ctccgtgact aaacaactta
    24421 atgtcatcaa acgcttcctg catgaagcca gcgaaatcgt tcacgccgga gacccggatc
    24481 gcgaagggca actgctggtg gatgaagtgc tggactacct gcaactggca ccggaaaagc
    24541 gccagcaggt acagcgttgt ttgataaacg acttgaaccc gcaggcggtt gagcgggcga
    24601 tcgatcgtct tcgctccaat agcgagtttg taccgctgtg cgtttctgca ctggcgcggg
    24661 cgcgggctga ctggctgtac ggcattaata tgacccgtgc gtataccatt ctcggccgca
    24721 atgccggtta tcagggtgtg ctttccgtgg gacgcgtgca gacgcccgta ctcggactgg
    24781 tggtgcgccg cgacgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag
    24841 cacatatcgt gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt
    24901 gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg
    24961 ttaaccgtat tagtggtcaa ccagctattg tcaccagcta taacgataaa cgggaatcag
    25021 aatccgcgcc gctgcctttt tcgctttcgg cgttgcagat tgaagcggct aaacgttttg
    25081 gtctgagcgc gcagaacgtg cttgatatct gccagaagct gtacgaaacg tacaagctaa
    25141 tcacttatcc gcgttccgat tgccgctatt tgccagaaga acattttgcc ggacgccacg
    25201 cggtgatgaa tgctatcagc gttcatgcac cggatctgtt gccgcagcca gtggtagatc
    25261 cagatatacg caaccgctgt tgggatgaca aaaaggtcga tgcgcaccac gccatcattc
    25321 cgaccgcacg gagttctgcg atcaacctga cggagaacga agcgaaggtc tataacctga
    25381 ttgcccgtca gtacttgatg cagttctgcc cggatgcggt gttccgcaag tgtgttatcg
    25441 aactggacat tgccaaaggc aaatttgtcg ctaaagcccg ttttcttgct gaagcaggct
    25501 ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa cgacggcacg ccgctgcctg
    25561 tggtggcgaa aggcgatgaa ttgttgtgcg aaaaaggcga agtggtagag cggcaaaccc
    25621 agccgccgcg ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct
    25681 ttgtgcagga caaagatctg aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag
    25741 caacgcgtgc cgggattatt gaactgttgt tcaagcgggg tttcctgacc aaaaaagggc
    25801 gctatatcca ctccaccgac gccggaaaag cgctattcca ttcgctgcca gaaatggcga
    25861 cgcgaccgga catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt
    25921 gtcgctatca ggactttatg cagccgctgg tggggacatt gtaccagttg attgatcagg
    25981 caaagcgcac gtcggtaagg cagtttcggg gaataatggc tcccggcggt ggagaaggga
    26041 agaaaaagga ttcgccacgc aagagagcgc cgaaaaaaag cccgccatca gaagaggcgg
    26101 gcaatggagt gataatataa aggttctttc aaccatctga tactgaagat ttttatggcc
    26161 ggataaggcg tttgcgctgt atccggcaca attgtcttaa atcacgccct ggcttaacat
    26221 cgcatccgca accttcacaa aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc
    26281 accttcacca ccatgctcaa cacaggcatg gtggatatcc agcatgatgt gatgcaaacg
    26341 tgcgtcaact ttctcggctt tccagcccag gcgcgcagcg ttttgcgcca tttccaggcc
    26401 cgatgttgct acaccgccag cattagccgc tttacccggt gcaaacagta cgcctgcctg
    26461 ctggaacagt tcagtcgctt cgatggtggt cggcatattt gccccttcgg cgacggcttt
    26521 aacgccattg gcgataagct gatgcgcggc gtcaacatcc agttcgttct gggtggcgca
    26581 aggcagggcg atatcaaccg gtacagacca cggttgttgg ccttcgagat agaccagacc
    26641 aaattctttg gcgtaatctg ccactcgacc atcgcggctg gctttgattt cgataaggcg
    26701 cgccagtttc tctttcgtga atccgctttc atcaactaca gtgccgctgg agtctgacgc
    26761 agtgatcact cgagcaccaa attccatcgc tttttcgata gcgtactggg cgacgttgcc
    26821 ggagccagaa acggaaacgc gcatcccttc aaaacccata ccgtggcgtt ttagcattgc
    26881 ttctgtgaaa taaaccagac cgtagccggt agcttccggg cgaataagac tgccgccaaa
    26941 tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt tcatcatccc
    27001 cgccataaag ccgacttcac gaccaccaac gccgatatca cctgccggaa cgtcagtatc
    27061 cgcgcccagg tggcgataca gttcagtcat cagcgcctgg cagaaacgca taacttcgcc
    27121 ttcgcttttc cctttcggat caaagtcgct gccgccttta ccaccaccca tcggcagagt
    27181 agtcagggca tttttaaagg tttgctcgaa gccgaggaat ttgagaatgg aaaggtttac
    27241 tgaagggtgg aagcgcatac cgcctttgta cggaccgatg gcggagctga attgcacacg
    27301 ccatgcacgg ttgacctgca cctggttgcg atcatcaacc catactacgc gaaattggat
    27361 cacgcgctcc ggttcaacca gacgctccag taatgacatc tgacgatatt tgggattttg
    27421 ttcaagaaaa ggccagagtg tggtcattac ttcacgaacg gcttgcgcga actcggtttg
    27481 attcgggtcg cgcttttgga catggttgag gaatgactcc agagaatatg tctgatccat
    27541 agatataaaa cccttatata ttaatacgat tatgcttatg ttgtttatgt catgtgcttt
    27601 tgcagttttc gactatacca tcaagaatgt gacggtaagc aagaaaaaaa tgacccagga
    27661 aagcaaatta ataacgagag taatctcata accaaatgcg ataactcaga cgctaaagtt
    27721 ttgccgcccg gctaccgtta tctgtagaag agacaataac gttaaggagt tccctgtgag
    27781 tcgagcattg atcgccgtag tattagcatt tccattaatt acactggcta acccgcatta
    27841 tcatccggac gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc
    27901 gcagccctgt actcagtgct gcgtttatca ggatcaaaat tattctgaag gggcagtaat
    27961 taaagccgaa ggaatactgc tgcaatgcca acgggatgac aaaaccctaa gcactaatcc
    28021 gctggtctgg cggcgcgtga agccataaac gcctctaata atggaatgtc agcaggggcc
    28081 agcggatatc gcagcgcctc ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc
    28141 tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa gatggataat ccgccccgaa
    28201 acttctcgct gatggctggc aacatagtca ccaacagttg cttcgatgcc cagttcttcg
    28261 tttaactcac gcaccagcgc ctgccgctgg ctttcatccg gctcgatttt accaccggca
    28321 aactcccata atcccgcctg atcgctctgg gcagggcgtt gcgcgagtaa aattttgcca
    28381 tcacgttcga tgatggcggc aacaacttca atcattttca taaattacac acgggcagat
    28441 aagcgaaaaa tgccataata tccgcactgt tgctcaatca gaaaggcttt atatttaagg
    28501 agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt
    28561 gcgggttctt gataaaccgg gcattacgcc agatggttta acattagtgg gatttgccat
    28621 cggcgtgctg gcactgcctt ttctggcgct gggctggtat ctggcggcac tagtcgccat
    28681 tttgttgaac aggctgcttg atggtctgga cggtgcgctg gcgcggcgca gagggcttac
    28741 cgacgcgggc ggctttctcg atatttctct cgattttctt ttttacgcgc tggtgccttt
    28801 tgggtttatc ctcgccgcac cggagcaaaa cgccctggca ggcggctggc tgctgtttgc
    28861 gtttatcggc acgggcagca gttttctcgc ttttgctgcg ctggcggcga aacatcagat
    28921 tgataatcct ggttatgcgc ataaatcgtt ttattatctg ggcggcttaa ccgaaggcag
    28981 cgagacgatt ttgctgtttg ttctgagttg cctctttccg gcctgttttc cgtggtttgc
    29041 gtgggtattt ggtgcgttgt gctggatgac gacctttacg cgggtgtgga gtgggtatct
    29101 gacgctgaag tcgctccagc gtcagtaatt gcaatgttat ttactgctgt ccgggccgcg
    29161 ttcaccggtt gctaccggat tttttggatc gctgctccat tcgtaccagc caccgtcgta
    29221 aacggagacg ttattccagc ccattgcgcg tgcgtacata aaggtttctg acgcgcgcca
    29281 gccagtgccg cagtagaatg aaacttgctg atctggtttg atattccatg ctttccacat
    29341 agcggttata tcatcggcgc tgcgcatggt gccatccggg ttatggaaat cttccatatg
    29401 cgtcgagtcg ctacctgcgt gtccccaacg tgctccggct atttcacctt ttggtttaat
    29461 atagctgtaa ccgctggtcg taccgataaa ttctggccac gaacgaatgc tcaccagcga
    29521 tgcatcctgg cgatgcagca gtccacgcgc ttgttccata tcaagcatca gttgcggttg
    29581 tgccgggatc ttcacgccga aatccggttc cgctttcact ttcggtggcg taccgcgctc
    29641 aacgggcaga cccgcgtcgg accaggtttg ccagcctccg tccagcaggc gcacatcttt
    29701 cacgccggca taaagcataa tttgcgccac acgcgctgca gcgtatacgt cacgcccgta
    29761 cagaatgacc gtggtgtcat ggcgaatgcc gtgttttgcc agcatcgctt tcagttgttc
    29821 atcagaaact ttgttccaca gcggttcgct ttccacttcg ttggtgtcga tgtagtcagc
    29881 gccgggaata tggctgataa ggtaaaattt tggagcgccc caggccgctt caatgacttt
    29941 ccagtcaccg gcaggtttcg ccgtaacctc tttaccttgt tgcaggtcat gcagccactg
    30001 cggatatacc agctgttcaa agtgcggcag tttttgcaga cgggaaggtt cgcttagcgc
    30061 gtcactcagg atggagatat gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc
    30121 gacatctttg tcattaccgt acagtgccac cggggcatcg gctttcaggt tatgttgctt
    30181 gatccactcg ttgagctgtt cggtgctcat tttgtcgagc cagctagcag agaggtttaa
    30241 ggcggcaggt tcatgaccgg aagggccatt taaggtttgt ggccagccgt tataaaacgc
    30301 gctggggcga gtatctatcg ctttgccatt ttgttgttga agctggtcaa gtgtaagagg
    30361 cttcgccagt tcagcggccc acgaagaagc gcaagccagc cctaaagcca ttgccagcgc
    30421 ggtcatttga gaaacacgtt tcatcgaaaa tcccgacatt gtagttttga gagcgccatt
    30481 ctgaactgac gcatcgggaa aaactttgcg ttatcgcagt ttgttgtaat tttctgacca
    30541 ctgcgccata tccagaacgg ggctatcagg aggaacatcc tgaagatcgt gcgtcacctg
    30601 cacaacaggg atcgccatgg cgcgaatttc gctgaacacc caatggcgaa aattatcgcg
    30661 cagggccaca tcaagacggc tgaatggctc atccagtagc aacgcttttg gctgagcgag
    30721 aagggcgcga agcagagcaa cgcgcgctcg ctgaccgcca gacaaagtgg caggatcctg
    30781 atggaacgct ccgtcaaggc ctgaacgctc aagtgcatca ttcacggcat tacgtcgggc
    30841 attcccctta agtgtcgccg gtagcgccag cagtaaattt tgcccgacgc tgaactggtc
    30901 aaataacagt gcatcctgga aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc
    30961 aatccgttgc tcattaagcc atagctcccc tgtacaagaa aactgtccgg ccagcgcacc
    31021 aatcatccac gaaaacagag tggatttccc gcagccggat ggtcccatta acgtgacaat
    31081 gtcaccttta tctactgtaa agttaacgtt tgtcagcaag cggctttctg gtaaacgtag
    31141 cgaaacattt ttcacgcaga gcattagcgg agtccttgtc tgacataacc tacccatttt
    31201 acgactaacg cggtcagggc aaaaataata agcggtaaca gcagttgcca taaagcctgg
    31261 gcggcgagaa taccgttgct gccgccgctg cttaatgcca ccgcttcagt ggtgagcgtc
    31321 ggaaaacgcc ccgcgcccag ccacagcgtc ggcatatact gcgcaatact gactgaaaat
    31381 cccaccgcga aggcaatcag cgcagggcgc aacatgagtg ggcattttac gtaaaagaag
    31441 attttggccc gcgaccagcc cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc
    31501 tgccaggcgg gttgcaggat aaacagcatc cacggcatta cccacagcag atgcccccag
    31561 accaccgccg tccagcttcc atccaggttc agccatagcg ccagcgtgta ctggcccgcc
    31621 accagcggca gagcgggtaa taaaatgggc aaccatagcc acaactggcg acgctgtggc
    31681 ccccattcca gccacagcag taacaagagc aaagcgatga atgccgccac cagccccatt
    31741 gtcaggctgt tgatgagcgc ttgactattg atcgtcgact gatccgcgag gatcgccagc
    31801 agaaccacac acagcacacc ggttaagggt aaaaaactcg ccagcgtatt gcttggcaat
    31861 aaaggcgtgg caggcttacg aacgccatct acgcggggaa tagtacgccg ccagccacgc
    31921 catagcagat agcccagcaa aacgtaggcg gcgagtaaca gcatcagcag caggctggcg
    31981 agtgcgcctt tcgtttgttg atcggcgtcg ccctgagtta accactgcca gctaattacc
    32041 gccagcgtcg ggggattacc tggcccaaga ataatcgcca catccacgac cgacagcgac
    32101 caggcgacaa tcgccagcat cgccattgct aacgcagacg cgacggaggg caacagcagc
    32161 cagttcaggc attgccagcg gctgtagccc agcgaatcca gcacaatgag ttgctgcaac
    32221 agccgttttt cgctcaatac tgccgctaag atccacagca gaaatgcgct ttctttcacc
    32281 gccagggtga ggcccagccc aatacccaat ttgtccattg gcggagtgaa atacgggaaa
    32341 tagtcataaa gcagccctcc gtcagcaaag agcagaaggg cgctggtggc aaaagccaca
    32401 tggggaatgg cgagcagcca cggcagacgg gcgcacatac gctgccattt cggccctggc
    32461 cacaacgcca caatcaccag cagggcaatc aacaacgccc cgaccgccgc aatggttgtc
    32521 gacaccagcg tcgccagtaa tgcctgcggt aactgtggat cggcaaataa cgcctgccag
    32581 tgcgtcaacg acaaagcagg cgatatcagc gtgagagctg ccgggatcag cggtgcataa
    32641 atcaccgcta tcatcgccca cagcaaaaag attaatgcgt accgtaacgg tgtagccatt
    32701 cttgttccag cgcatttacc caacctgcgt gcggttcagc cagtaccggc ggcagatcct
    32761 gcggcattct cgattgcaat gtttcgcgct gcccgtcagg cagtttttgc gggtcgagaa
    32821 cagaaggatc gccccagaca gcaggatctg ctttacgcag ttgcgcatcg ggtgagagca
    32881 ggaaattggc aactaccttc gccgcagcac tggcattcgc gttggcagga atggtgacga
    32941 aatggacgtt gccaatcatc ccctcgcgaa aaccaaaact gtaactgctt gcaggcaaat
    33001 cgccgctggc gattttttgc tgcgcatgcg cggggttaaa ggtcagcgac aggcgcaatg
    33061 tgttagcttt cagcagagca tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc
    33121 gccacaaata cggatgcagt gcatcaagat attgccacaa gggcgcagtg acgcgggcga
    33181 aagtggcatc gtccggcgct tcttttaatg ctgtgggatc tggcgttagc ataatcagca
    33241 actgttcaag aaacgccgtg ccggtaaagt ccggtgggcg cggataggta accgtgccgg
    33301 gattggcttt agcaaactcc agtaaggctt gcggcgtttg tggtggctgt agcgtaacat
    33361 cgcggcgggc gataaacgtc agttgtgcgc cgccccaggg cgattcagcc ccttctgtag
    33421 gcactgaaaa atcttcccgc accggaagct gtgtgtcgac atagcgccag ttgggcagag
    33481 tctccgccca gccggtttgt aataaattgg cctcttttaa ggtgcggaag ttttcgccgt
    33541 tcacccagag cagatccacc gaaccgcccg ttttacgtcc ggcagcaact tcggtctgaa
    33601 tgcgtttcac cgcgtctgcg gcatccgcga gacgaacaat cttcaggttt atagcgtaat
    33661 gggttttcat ctcgccgcta acccagtcga gatagcggtt aattgcggta tcgccgcccc
    33721 aggcgttaaa ccagacggtt tgtcctttgg cctcattttt tatttcctgc cagtcactgg
    33781 catgtgttgc caaagaaaac agcgataaca atcccagcaa ccacccacaa tggcgcatct
    33841 tctctcctta agaacgtttt tcaggaatac ggcacagccc ctgagcggga atgtccatgg
    33901 cagaattaaa acgggctgac agattttgca ggcctatcaa tgccgtcagt tcggtgagcg
    33961 cctgagcgtc aaaatgcgta gccagtcggg cgcgcagagc atcatcgacc gttggcggcg
    34021 ttacgcttgc ggcttcggcg tactccagcg ccagccgttc ttcatcgcta aagagcgggc
    34081 tttgccgcca gtcagccact gccagcagtt tatcggtgct gccggtacgc tcggcgactt
    34141 tcatactggt gatatccacg caaaactcac acaggcacat ttgcgcaatg cgcgcgctga
    34201 caagcgatcg tactaccgga tcgagcggtg agcgtttgcg ctccagccag ccgacaaaca
    34261 tcgacaccag ataaaagata aacgggatcc gcccccacca gcgaatcggg cttagcacct
    34321 cgccgtaatg gcgacgatgc agccacgcct gcggacgtaa aatgaaagga attttgctaa
    34381 gcggcggtaa acccatcagg cattcctctt ctggcgctca agccatattt tcttcgccat
    34441 aaaaatcacc acggtcaggg caaacagaat aaacagtccg gtgacaaacc agaaagtacc
    34501 gcctgttaac atgctgcccg cccaggaata aacaatagtc gccggtaatt gaccaagccc
    34561 ggtggcgata aaaaacgagc gaaaacgtat tgaagtcaaa ccggcagcat agctgattgg
    34621 atcgaaaggg acaaaaggca ataaccgaca taccaggatg gtgtgtttgc cgtagcgagt
    34681 gaaaaagccg tccatactgt caagtacggt tttgccggtt aatttttcca ccacttcgcg
    34741 gcccatcact ctggcgataa aaaagcacag cgccgcgccg gccatcgaac tggtccacga
    34801 cagtaagccg ccccagaacg cgccaaacag cgacgcattg gcaaaggtga tcaaaaacgc
    34861 aggtagcggt gcagcaatgg cctgtaaaat catcaacaag aacgagacaa ctgccgctag
    34921 tgcgccgtaa gactgaataa agcgttctat accctgttgg tccacggcgg caaacgccgc
    34981 aacgctgcga ttgataaact catggacgcc gggaagcagc gcccatgcca gcagcacagc
    35041 gaacaacagg atgatgaggg ttatacggta gtaccagatt ttacgcgatt gcatcttcat
    35101 catcctttat cctgctttat tcggtgattt taaaggtaac cgttgccagc gtgttatccg
    35161 ccgggagaac tgaggcattg cctttgaatt tcacttcgcc gcgtttttcc accgcaccat
    35221 aagtgtatgt tgcattgctg acgatgccga ccgggcagct atccagacac accaggcaac
    35281 ccgttttctt ctcttctgct gccgttaaat taccgccaaa gcgcatgtcc agtttcttgc
    35341 cattactgtc aacaatcact tcatcgaagg aatacgcttt tgccgcccct tgccagttga
    35401 ccgaaatatc cagtttgctg cctgtgacat gagtcgtttc tttattatcc attgtcatgt
    35461 tttcgcccgg cgtaccacct gcctctttca gggcttcata aaatgctttc ggggtcgcat
    35521 aacccataaa cagcgattta tgtccgttgg agccatcttt aaacacaata ccgtgacgag
    35581 tgtcgtcggt gaggaaacga ccattaattt gcaccagcat ggttaccgta tggttattgg
    35641 catcgacacg cagcggttgg ctgttcgaaa gtccgtcgta ttccactttt gcagccgcat
    35701 tctctttttg atcgcagccg ctaagcccag caacagccag taagcagagt gcagccagtc
    35761 cttttttcca tgacactgaa taatgttgca acatcgttct atccttcatt ttttgggtga
    35821 gaaagtgggc tgcggcgaga agcagacaga tccacatgtt tgtgtcgggc gtagagtttt
    35881 gcgagctgga cgagaataaa cagtgccagg cccgccagac agagttgtaa aataaagcgc
    35941 agcgtaatgc cttcattggc gagatcgctt gccatcacgg tataaataac aataccgggt
    36001 agggtcgtga gtgccgaaat aagggtataa ggccagaagg cgattgtggt taatccgtaa
    36061 gcgtaatttt gaatattgta aggaaacaac gggattaagc gggtcagaat aagaaaatca
    36121 ataccgttac gcgcaatgcc tttttcaatg gcctgaaagg tatggctatg gccaacgtat
    36181 ttcagcagta aatcacgtcc cagccagcgc gccagaagga atgagcacga ggaggccagc
    36241 gtggcggcaa ttaatgatag tagtgtccct aagagcgggc caaaaactat tcctcccgcg
    36301 atcaccagta tgcttcctgg taacagaaag agggtggcaa tgatgaataa cagaatatag
    36361 aggctatagc cgaacaatcc gctctggcga atgagtgtct gtaagtgggg taaatcggtg
    36421 agcagatcga ataaaccgaa agcgtggata gcgtaaatga ccagcgcaaa aataagacag
    36481 gcaaaaagaa atttacgctc agcgttcatc gtggagtttc ctatatcaaa cttgataata
    36541 gagaagtggt cagacaacta aaaacgcagt gacggtataa caaaggacgg cgggcaacaa
    36601 atcattactg acagtttgtt gatttttctc aaggttaatt ctcctgaccc agtttgagcc
    36661 aggagatcag ctaaattagc ggcggaaggt cgcccagacg ggggcgtgat cggacggttt
    36721 ttccatgctg cgaatttcat agtcgatgcc ggtttctacg caacattctg ccagcggttg
    36781 gctggcgagc agcaggtcga tgcgcagacc gcgattatcg tcaaaacctt ttgagcggta
    36841 atcaaaccat gagaaacgat ctgctgtttg cggattcgca tggcggaagg tatcgaccaa
    36901 cccccagctc atcagcctct ccatccattc gcgttcctcc ggaaggaaag aacattttcc
    36961 ggtacgcagc cagcgcttac ggttctcttc gccaatgcca atatccaggt cgccaggact
    37021 gatattcata tcgcccataa tcagcaccgg attttcacgc ttgagttcgg tttccaggta
    37081 gttttgcaga ttctgataaa actgcgcctt tgccgggaat tttatcggat ggtcgcggct
    37141 ttcaccctgc gggaagtaac cgttgatcac ggtgatattg cccagcggcg aggggatttc
    37201 cgccataata atccgccgtt gcgcctcttc gtcgtcaccg ggaaagccgc gacgcacgga
    37261 aatcggcgtc tctttggtca gcagcgccac gccataatgg cctttctgcc cgtgataaaa
    37321 cacgttgtag ccgagcttcg ccacctcttc gagcggaaac atatcgtcat gaacttttgt
    37381 ctcctgcagg ccaatcacat ccggttggtg cttttcgacg atggcttcaa gctggtgagg
    37441 tctggcgcgc aggccgttga tattaaaaga gacaaatttc atagtcgctg ccatttattc
    37501 agataaagag tgcgtggatg gtagcagaat ttcgctttgc ttaccgcctg ttgttaagtg
    37561 atttgatcga tggttgttaa tgatgtcaac gatggcgcaa aaaatgcccg cttttggtgc
    37621 gcgttgcgtc agaatggcgc agtaatttcc agtaaatttc gtcaatgatc acaattccag
    37681 aattatattt tacttctgca taacattgcg ttttttattc tttttattca cccacatcgc
    37741 aaacgtattc actttatatg cactttaaat gcatatgatg tggttataac tgcttgattt
    37801 ccagttagcc tccgccgttt atgcactttt atcactggct ggcacgaacc ctgcaatcta
    37861 catttacagc gcaaacatta cttattatta acatataaat aactaattat ttactgtaga
    37921 ggtcgctatg tctcagccaa ttacgcgtga aaactttgat gaatggatga tacctgttta
    37981 cgctccggca ccgtttatac cggtacgtgg cgaaggttcg cgcttgtggg agcagcaggg
    38041 gaaagagtat atcgacttcg cgggtggcat tgcggtgaac gcgctgggcc atgcgcatcc
    38101 ggaactgcgt gaggcgctga acgaacaggc gagtaagttc tggcataccg gcaacggtta
    38161 caccaacgag ccggtactgc gactggcgaa aaaattgatc gacgccacgt ttgccgatcg
    38221 cgtcttcttt tgtaactccg gtgcggaagc caacgaagcg gcgctaaaac tggcgcgtaa
    38281 attcgctcac gaccgctacg gcagccataa gagcggcatc gtggcgttca aaaatgcgtt
    38341 tcatggtcgc acgctgttta ctgtcagtgc gggtgggcag ccagcctatt cacaggattt
    38401 tgcgccactg ccgccggata ttcgtcatgc tgcatataac gatattaact ctgccagtgc
    38461 gctgattgac gacgccacct gtgcggtgat tgtcgaaccc atccaggggg aaggcggtgt
    38521 ggtgccagcc agcaacgcat ttttacaagg tctgcgtgaa ttgtgtgacc gccacaatgc
    38581 gttgcttatc tttgatgaag tacaaaccgg tgtcgggcgc accggggaac tgtatgccta
    38641 tatgcactac ggcgtgacgc ctgatctgtt aactaccgcc aaagcgctgg gcggcggttt
    38701 cccggttggt gcgttgttgg caaccgaaga gtgcgccagc gtgatgaccg ttggcactca
    38761 cggcaccacc tatggcggca atccgctggc ctcggcggtg gcaggcaaag tgctggatct
    38821 catcaacacg ccagagatgc ttaacggggt taaacagcgt cacgactggt ttgttgagcg
    38881 tctaaattcc attaatcacc actacagttt gttcagtgaa gttcgcggct taggtctttt
    38941 gattggctgt gtactgaatg ccgattacgc cgggcaagcg aaacagctct ctcaggaagc
    39001 ggcgaaagca ggcgtgatgg tgctgattgc gggtggcaac gtggtgcgtt ttgcgccagc
    39061 gctcaatgtc agcgaagaag aggtgacgac cggactggag cgctttgcag ctgcttgcga
    39121 acactttgtt agccgaggtt catcatgatg gtcattcgtc ccgttgagcg ctcagatgtc
    39181 tcggcgctga tgcagcttgc cagcaaaacg ggcggcggcc tgacgtcgct tcccgccaat
    39241 gaagccacgc tttcggtgcg tattgaaagg gcaatcaaaa cctggcaagg cgaactgccc
    39301 aaaagtgagc agggttatgt gtttgtactg gaagatagcg agtcaggcac tgtggcgggg
    39361 atttgcgcca ttgaggtggc ggttgggctg aacgatccct ggtacaacta tcgcgtcggc
    39421 acgctggttc acgcttcgaa agagctgaat gtctataacg cattgccgac gctgtttctc
    39481 agtaacgatc acaccggcag cagcgagctg tgcacgctgt ttctcgaccc ggagtggcgc
    39541 aaagagggca acggctattt gctgtcgaaa tcgcgcttta tgtttatggc ggcttttcgc
    39601 gacaagttta atgacaaagt ggtcgccgaa atgcgcgggg tgattgacga acacggctat
    39661 tcaccgttct ggcaaagcct cggtaaacgc ttcttttcga tggattttag ccgcgctgat
    39721 tttctctgcg gcaccgggca aaaggcattt attgcagaac tgatgccgaa acatccgatc
    39781 tatacgcact ttttatccca ggaagcacag gacgtcatcg gtcaggtaca tccgcaaacc
    39841 gcacctgccc gtgcggtgct ggagaaagaa ggttttcgct accgtaacta tatcgacatc
    39901 tttgacggcg ggccgacact tgagtgtgat atcgaccgcg tgcgcgccat ccgtaaaagt
    39961 cggctggtgg aagtggccga agggcagcct gcgcagggcg attttccggc ctgcctggtc
    40021 gccaatgaaa attatcacca tttccgcgtg gtgctggtgc gtaccgatcc agcgaccgag
    40081 cgtttgattt taaccgccgc acaactggat gccctcaaat gccacgcagg ggatcgcgtt
    40141 cgtctggtgc gcctgtgcgc agaggagaaa acagcatgac tttatggatt aacggtgact
    40201 gggtaacggg ccagggcgca ttgcgtgtga agcgtaatcc ggtatcgggc gaggtgttat
    40261 ggcagggcaa tgatgccgat gctgctcagg tcgagcaggc ttgtcgggct gcccgagcgg
    40321 cgtttccgcg ctgggcgagg ctctcattta gcgatcgtca ggtaagagtt gaacgctttg
    40381 ccggattgct ggaaagcaat aaagctgaat taaccgcgat tattgccaga gaaaccggca
    40441 agccgcgctg ggaagcggca accgaagtga cggcgatgat caataaaatc gcgatatcaa
    40501 ttaaggcgta tcacgttcgt accggtgagc agcgtagcga aatgccggac ggcgcggcga
    40561 gcttgcgcca tcgtccgcac ggcgtactgg cagtgtttgg accgtataat ttccctggtc
    40621 atctgccgaa cggacatatt gttccggcat tgctggcagg taacaccatt atttttaaac
    40681 ccagcgaact gacaccgtgg agtggcgaag cggtaatgcg tttatggcag caggctggct
    40741 tgctgccggg cgtgctgaac ctggtgcagg gcgggcgtga aacgggtcag gcgctgagtg
    40801 cgctggagga tctcgacggt ttgctgttta ccggcagcgc caataccggc taccagctgc
    40861 atcgccagct ttccggtcag ccggagaaaa ttctcgccct tgagatgggc ggtaataacc
    40921 cgctaattat cgatgaggtg gcggatatcg atgccgctgt ccatctgacc attcagtcgg
    40981 cgtttgtcac tgccgggcaa cgctgcacct gcgcccgccg tttattgctg aaaagtggaa
    41041 cgcagggcga tgcatttctt gctcgcctgg ttgccgtcag ccagcggtta acgccgggca
    41101 cttgggatga cgaaccgcag ccgtttattg gcgggctgat ttctgagcag gccgcacagc
    41161 aggtggttac tgcctggcag gaactggagg cgatgggcgg acggcccctg cttgcgccgc
    41221 gcttattaca agcagggaca tcgttgctga cgccggggat cattgaaatg acaggcgtta
    41281 ctggcgtgcc ggatgaagaa gtgtttgggc cgttattgcg cgtctggcgt tatgacaatt
    41341 tcgatgaagc gattcgaatg gcgaataaca ctcgcttcgg cctctcttgc ggtctggttt
    41401 cccccgagcg ggaaaaattc gatcaactgt tgctggaggc acgggcgggg attgttaact
    41461 ggaacaaacc gcttaccggt gctgccagta ccgcgccatt cggcggcatt ggcgcttccg
    41521 gcaatcatcg ccccagcgcc tggtatgccg cagattattg tgcctggccg atggcaagtc
    41581 tggagtcgga ctcgttaacg ttgcccgcaa cgcttaaccc cgggctggat ttttccgatg
    41641 aggtggtgcg atgaacgcct gggaagtcaa tttcgacggg ctggtagggc tgacgcacca
    41701 ttacgcgggc ctgtcgtttg gtaatgaagc ctctacccgt caccgttttc aggtgtctaa
    41761 cccgcgactg gcggcaaaac agggcttact gaaaatgaaa aaacttgccg atgcgggatt
    41821 cccccaggcc gtgatcccgc cgcacgagcg tccgtttatt ccggtgctgc gtcagttggg
    41881 attcagtggt agcgatgagc aggtactgga aaaagttgca cgccaggcac cgcactggct
    41941 ttccagcgtc agttccgctt cgccaatgtg ggtagccaat gcggcaacga tcgcgccatc
    42001 cgccgatacg ctggatggca aagtgcatct cactgttgct aacctgaaca ataaatttca
    42061 ccgttcgctg gaagcccccg tcaccgaatc gctgttaaaa gcgattttta acgacgaaaa
    42121 gaaatttagc gtccattcgg cgttgccgca ggtagccttg ctcggtgatg agggggcggc
    42181 aaaccacaat cgtctcggcg gtcattacgg tgaaccgggt atgcaacttt ttgtctacgg
    42241 gcgagaagag ggcaatgata cccggccttc ccgttatccg gcgcggcaga cccgtgaagc
    42301 cagcgaggcg gtggcaaggt tgaatcaggt gaatccccaa caggtgattt tcgcccagca
    42361 aaacccggac gttatcgacc agggcgtttt tcataatgac gtgattgccg tgagtaaccg
    42421 ccaggtgctg ttttgccatc aacaggcgtt cgctcgccag tcacagttac tggcaaatct
    42481 gcgtgcacgg gtcagtggtt ttatggcaat agaggtgccc acggcgcagg tttccgtgtc
    42541 agatgcggtg tctacttatc tgtttaacag ccaactgctg agccgcgatg atggttccat
    42601 gatgttggtg ctgcctcagg aatgtcggga acatgctgga gtatggtgtt atctcaatga
    42661 attgcttgct gccgataacc caataagctc actaaaagtt tttgatttac gtgaaagcat
    42721 ggcgaatggc ggtggcccgg cgtgcctgcg gctgcgcgtg gtgttgacag aagaagaacg
    42781 ccgggcggtg aatccggcgg tgatgatgaa cgatacgctg tttaatgcgc tcaatgactg
    42841 ggtggatcgt tactaccgcg atcgtcttac tgcggcggat cttgccgatc cacaattgct
    42901 gcgcgaagga cgggaagcgc tggatacact gactcaatta ttggatcttg gttcggttta
    42961 tccattccag cgcgagggag ggggaaatgg ataattttct tgctctgacc ttaacgggta
    43021 aaaaaccggt tatcaccgag cgagaaatca acggcgttcg ctggcgctgg ctgggcgatg
    43081 gcgtgctgga actgacgcca ttaacgccac cgcaaggcgc actggtgatt tcagcgggaa
    43141 tacacggtaa tgagacggca cctgtggaaa tgctggatgc gttgcttggc gcgatatctc
    43201 acggcgagat cccgttacgt tggcggttgc tggtgatcct cgggaatcct cctgcgctga
    43261 agcaagggaa acgttattgc catagcgata tgaaccgaat gtttggcggt cgttggcagc
    43321 tatttgctga aagcggggaa acctgtcggg cgcgcgaact ggaacagtgc ctggaagatt
    43381 tttatgacca gggcaaagaa tctgtgcgct ggcaccttga tctccatacc gcaattcgtg
    43441 gctccttgca tccgcggttc ggtgtattac cgcaacgcga cattccctgg gacgagaaat
    43501 ttctgacgtg gctgggtgcg gcgggtctgg aggcactggt gttccatcag gagcctggtg
    43561 gtacgtttac ccatttcagc gccagacatt ttggcgcgct ggcctgtacg ctggaactcg
    43621 gcaaagcgtt gccctttgga caaaacgatc ttcgccagtt tgcagtaact gccagcgcaa
    43681 ttgctgcgct gctatctggt gagagtgtcg gtatcgtgag aacaccgccg ctccgttatc
    43741 gggtggtttc gcaaattact cgccactcgc cgtccttcga aatgcatatg gcaaatgaca
    43801 cgctgaattt tatgccgttt gagaaaggaa cattgctggc gcaggacggg gaggaacgtt
    43861 ttaccgtaac ccatgatgta gagtatgtgt tattccctaa tccgttggta gcgttgggat
    43921 tacgtgcggg attaatgctc gaaaaaataa gctaatactt acccgcagaa atcattctgc
    43981 gggtattaag ttgctttctt ataaattaat acagattaat cct
//
LOCUS       DACADA010000022        43786 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.022, whole genome shotgun sequence.
ACCESSION   DACADA010000022 DACADA010000000
VERSION     DACADA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.022"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(87..3236)
                     /locus_tag="ISB17_001180"
     CDS             complement(87..3236)
                     /locus_tag="ISB17_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP1979135.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3259..4452)
                     /gene="acrA"
                     /locus_tag="ISB17_001181"
     CDS             complement(3259..4452)
                     /gene="acrA"
                     /locus_tag="ISB17_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAP1979136.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4594..5228
                     /gene="acrR"
                     /locus_tag="ISB17_001182"
                     /pseudo
     CDS             4594..5228
                     /gene="acrR"
                     /locus_tag="ISB17_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
     gene            5356..8718
                     /gene="mscK"
                     /locus_tag="ISB17_001183"
     CDS             5356..8718
                     /gene="mscK"
                     /locus_tag="ISB17_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAP1979137.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8757..8921)
                     /gene="rsmS"
                     /locus_tag="ISB17_001184"
     CDS             complement(8757..8921)
                     /gene="rsmS"
                     /locus_tag="ISB17_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAP1979138.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(8935..9462)
                     /gene="priC"
                     /locus_tag="ISB17_001185"
     CDS             complement(8935..9462)
                     /gene="priC"
                     /locus_tag="ISB17_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAP1979139.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9532..9909
                     /locus_tag="ISB17_001186"
     CDS             9532..9909
                     /locus_tag="ISB17_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAP1979140.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10062..10613
                     /gene="apt"
                     /locus_tag="ISB17_001187"
     CDS             10062..10613
                     /gene="apt"
                     /locus_tag="ISB17_001187"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP1979141.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10742..12673
                     /gene="dnaX"
                     /locus_tag="ISB17_001188"
     CDS             10742..12673
                     /gene="dnaX"
                     /locus_tag="ISB17_001188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP1979142.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     gene            12726..13055
                     /locus_tag="ISB17_001189"
     CDS             12726..13055
                     /locus_tag="ISB17_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP1979143.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13055..13660
                     /gene="recR"
                     /locus_tag="ISB17_001190"
     CDS             13055..13660
                     /gene="recR"
                     /locus_tag="ISB17_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP1979144.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13770..15644
                     /gene="htpG"
                     /locus_tag="ISB17_001191"
     CDS             13770..15644
                     /gene="htpG"
                     /locus_tag="ISB17_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAP1979145.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15825..16469
                     /gene="adk"
                     /locus_tag="ISB17_001192"
     CDS             15825..16469
                     /gene="adk"
                     /locus_tag="ISB17_001192"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP1979146.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16601..17563
                     /gene="hemH"
                     /locus_tag="ISB17_001193"
     CDS             16601..17563
                     /gene="hemH"
                     /locus_tag="ISB17_001193"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAP1979147.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17560..18519)
                     /gene="aes"
                     /locus_tag="ISB17_001194"
     CDS             complement(17560..18519)
                     /gene="aes"
                     /locus_tag="ISB17_001194"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAP1979148.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18671..19975
                     /locus_tag="ISB17_001195"
     CDS             18671..19975
                     /locus_tag="ISB17_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAP1979149.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20105..21781)
                     /locus_tag="ISB17_001196"
     CDS             complement(20105..21781)
                     /locus_tag="ISB17_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAP1979150.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22020..23240)
                     /locus_tag="ISB17_001197"
     CDS             complement(22020..23240)
                     /locus_tag="ISB17_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979151.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23458..25110
                     /locus_tag="ISB17_001198"
     CDS             23458..25110
                     /locus_tag="ISB17_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAP1979152.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25148..25651)
                     /locus_tag="ISB17_001199"
     CDS             complement(25148..25651)
                     /locus_tag="ISB17_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979153.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25648..26448)
                     /locus_tag="ISB17_001200"
     CDS             complement(25648..26448)
                     /locus_tag="ISB17_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979154.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26472..26951)
                     /gene="ybaK"
                     /locus_tag="ISB17_001201"
     CDS             complement(26472..26951)
                     /gene="ybaK"
                     /locus_tag="ISB17_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAP1979155.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(27155..27949)
                     /locus_tag="ISB17_001202"
     CDS             complement(27155..27949)
                     /locus_tag="ISB17_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979156.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            28082..28384
                     /locus_tag="ISB17_001203"
     CDS             28082..28384
                     /locus_tag="ISB17_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1979157.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            28420..28761
                     /locus_tag="ISB17_001204"
     CDS             28420..28761
                     /locus_tag="ISB17_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP1979158.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(28819..31323)
                     /gene="copA"
                     /locus_tag="ISB17_001205"
     CDS             complement(28819..31323)
                     /gene="copA"
                     /locus_tag="ISB17_001205"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAP1979159.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            31585..32517
                     /gene="glsA"
                     /locus_tag="ISB17_001206"
     CDS             31585..32517
                     /gene="glsA"
                     /locus_tag="ISB17_001206"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP1979160.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            32520..33812
                     /locus_tag="ISB17_001207"
     CDS             32520..33812
                     /locus_tag="ISB17_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1979161.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            33937..34344
                     /locus_tag="ISB17_001208"
     CDS             33937..34344
                     /locus_tag="ISB17_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAP1979162.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(34345..34996)
                     /locus_tag="ISB17_001209"
                     /pseudo
     CDS             complement(34345..34996)
                     /locus_tag="ISB17_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35174..35632)
                     /locus_tag="ISB17_001210"
     CDS             complement(35174..35632)
                     /locus_tag="ISB17_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAP1979163.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(35629..36546)
                     /locus_tag="ISB17_001211"
     CDS             complement(35629..36546)
                     /locus_tag="ISB17_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAP1979164.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36692..37369
                     /gene="fetA"
                     /locus_tag="ISB17_001212"
     CDS             36692..37369
                     /gene="fetA"
                     /locus_tag="ISB17_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAP1979165.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            37356..38135
                     /gene="fetB"
                     /locus_tag="ISB17_001213"
     CDS             37356..38135
                     /gene="fetB"
                     /locus_tag="ISB17_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAP1979166.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(38318..39172)
                     /gene="cnoX"
                     /locus_tag="ISB17_001214"
     CDS             complement(38318..39172)
                     /gene="cnoX"
                     /locus_tag="ISB17_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAP1979167.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RVLLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(39233..40042)
                     /gene="ybbO"
                     /locus_tag="ISB17_001215"
     CDS             complement(39233..40042)
                     /gene="ybbO"
                     /locus_tag="ISB17_001215"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP1979168.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAVMVLKRLLPGRVMDKILQG"
     gene            complement(40032..40655)
                     /gene="tesA"
                     /locus_tag="ISB17_001216"
     CDS             complement(40032..40655)
                     /gene="tesA"
                     /locus_tag="ISB17_001216"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAP1979169.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            40626..41312
                     /locus_tag="ISB17_001217"
     CDS             40626..41312
                     /locus_tag="ISB17_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1979170.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            41309..43723
                     /locus_tag="ISB17_001218"
     CDS             41309..43723
                     /locus_tag="ISB17_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1979171.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT        10519 a        11452 c        11583 g        10232 t
ORIGIN      
        1 tgagttggtg gttcaataca tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg
       61 cgcggcctta gtgattacac gttgtatcaa tgatgatcga cagtatggtt gtgctcgata
      121 tcttcattct tgcggctaaa gcggcggcga accaccacaa agaataccgg aacgaagaag
      181 attgccagta ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc
      241 gcgccggaac cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccaac
      301 gaggtcatca ggattgggcg taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga
      361 cctttacctt ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc
      421 gccgacaaac caatggttgt gagcaggcct acctggaagt aaacgtcatt ggtcaggcca
      481 cggaaggtgg cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag
      541 aacggaatcg accagctctc gtacagcgca gccagacaca ggaacacgac aatcaacgaa
      601 atcgcgtaca gtgaaggtgc ctggttgccg gagagacgtt cctgatagga catccccgtc
      661 cagtcatagc caacaccggt aggcagtttg ctcgccagtt gttccatcag ctccattgct
      721 tcaccggtac ttttacccgg tgccgcctgg cctaagattt ccatagatgg caggccgttg
      781 taacgttcca gacgcggcga accgtactcc caacgagaag aggagaacgc cgagaacggc
      841 accatctgac catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg
      901 tatttcgcct ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc
      961 acatagctac cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc
     1021 agcgcctgcg ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg
     1081 tttggacgta cgctggtcaa catatcaggg tgcttcgctg cttctgcaag caactggtta
     1141 cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag
     1201 ccggttgcgg tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct
     1261 ttgatttgcg agaaagcacg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg
     1321 cccggacgat cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt
     1381 cccgcaaagc cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc
     1441 agatagtaat tcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct
     1501 ggcagctgaa ccatggtcat aaatacgccc tggtcctcat ctggcaagaa ggagcttggc
     1561 agacgcacga acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc
     1621 cccgtactgc gtagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac
     1681 atgcggttaa accagccgaa gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc
     1741 ggtttcagca tggtggcaca aagagccgga gtcaggatca acgccaccag taccgacagc
     1801 gccattgctg aaacaatggt aatagagaac tgacgataga tagcaccagt agaaccgcca
     1861 aagaaggcca tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc
     1921 tgaatctgcc ccatcgactt acgggtggct tcttttggtg gcaaaccttc ttccgccatc
     1981 acacgctcaa cgttttctac cacaacgatg gcgtcatcca ccaacaggcc gatggcgagc
     2041 accatcccga acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag
     2101 gtcccgagca ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc
     2161 aagaacagat acattaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact
     2221 tcgtgaatag agattttcac gaacggtgtg gtgtcatacg ggtaaacaat tttcagaccc
     2281 gacgggaaga acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc
     2341 gcgttcgcac cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct
     2401 gcgatgatgt cgtagttctc accacccagc tcaattttcg ccacatcacg cagcagcacg
     2461 cgggaaccat cctgattcac tttcagcagg attttgccga actcttcagt agaggtcaga
     2521 cgcgtctgag caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg
     2581 agctgaccag ctgcaacctg ggcgttctgt gctttgatgg cggtaatgac atcaaccggc
     2641 gttagctgga atttgttcag ttcattcggg ttcatccaga tacgcatcgc gtactgtgaa
     2701 ccgaacaact gaacgtcacc cacgcccgac gtacggctga tggcatcttt catattcgcc
     2761 gccacgtagt cggagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca
     2821 accatcagga agctgctgga tgatttctca acgctcaccc cttgctgctg tacttcttgc
     2881 ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatatccgca
     2941 tcagtaccag actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag
     3001 gacatgtaca tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg
     3061 tcctgcactg ttttcgcatc agcgccaggg taggaggcgg agatcgttac tgccggcggt
     3121 gcaatcgtag gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg
     3181 atgataatgg cgatcaccca cgcaaaaatt gggcgatcga taaagaaatt aggcatgtct
     3241 taacggctcc tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg
     3301 ctggttatta tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg
     3361 cagcccactt attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc
     3421 gccgatagcc tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaaccac
     3481 cagtacggtg gcatcgccac gtggcgtacg ggttacgccc tgttgcggga ctaaaatagc
     3541 gtttggatta agcccttctt ccagacgtgc acgcacgaac atacccggca gcagagtgtg
     3601 atccgggttc gggaagatag cgcgtagggt gatagaccca gtggtctgat caacggtaac
     3661 gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga
     3721 cactttggct ttgccgttct cttgtttcag cgtgccattc gccagttcct gtttcaggcg
     3781 caggaagtcg ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt
     3841 tgccagcgca gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt ttgacttacc
     3901 aatgcgacca ctaatcggag aggtgacttt ggtgtaagcc agattgattc gcgcagtttc
     3961 aacggcagct ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg
     4021 atcgtactct tgtttactga tgtactgagt accgagcaat ttctgataac gattcaccgt
     4081 caattgcgcg atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta
     4141 tgccgcctga taggtcgcag gatcaatctg atagagagag acacctgctt cgatgtcgct
     4201 accttctttg aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat
     4261 ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac
     4321 tgttactacg ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca
     4381 tcctgttagg gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct
     4441 gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt
     4501 aataaaccca ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt
     4561 atgtaaatct aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg
     4621 caagaaacgc gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta
     4681 tcatccacct cgctgggtga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac
     4741 tggcatttta aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat
     4801 attggtgaac tcgagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta
     4861 agagagatat taattcatgt tcttgagtcc acggtgacag aagaacggcg tcgattattg
     4921 atggagatta tattccacaa atgcgaattt gtcggagaaa tggggcacaa cgtaatctct
     4981 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5041 tgcctgcgga tttaatgacg cgtcgcgcag caattattat gcgcggctat atttccggcc
     5101 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5161 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaac cctgccacta
     5221 acgaataacc ctgaatctga ctccaggaat tttcctggac attttcgtcg ttgctattct
     5281 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5341 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5401 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcacgggcg
     5461 tcatcgaatg gtgatttacc gacaaaagcg gacctgcagg cacaacttga ctcactgaat
     5521 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5581 gccaccctcg ataaaatcga tcgcgtaaaa gaagagacag ttcagctacg gcaaaaagtc
     5641 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagtgatgtc
     5701 gataacgacg aagaaacgcg caaaattctg agcgcgcttt cgttgcgcca gctggaaact
     5761 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa atgatctggc gtcttataac
     5821 agccaactgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5881 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     5941 ttacgtccca gccagaaagt gttaatgcag gtccagcagg ctttgctgaa tgccgagatt
     6001 gaccagcagc gtaaaagcct ggaagggaac actgtcttgc aggatacctt gcaaaagcag
     6061 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttacaact gttgcaagaa
     6121 gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg cgcaggaagc tgtctccccg
     6181 gatgaagccg cgcgtattca ggctaatccg ctggtgaagc aggaactgga aattaaccag
     6241 cagttaagtc agcgtctgat taccgcgact caaaacggta atcagttgat gcagcaaaac
     6301 attaaagtca aaaactggct ggagcgtgcg ctgcaatcgg aacgtaatat taaggagcag
     6361 attgccgttc tgaagggcag tctgctgttg tcacgtatcc tgtaccagca acaacaaaca
     6421 ctcccctcgg cagatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6481 cagtttgaag tcaaccagca gcgtgacgcg ctgttccaga gcgatgcgtt cgtcagcaaa
     6541 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcatt attgcaagta
     6601 gttgatatgc gtcgcgaatt gctggatcag cttaacaaac agttgggtaa tcagctgatg
     6661 atggccatta acctgcaaat taaccagcag cagttaatga gcgtatcgaa aaatctgaaa
     6721 tccatcctga cgcagcaaat tttttgggtg aacagtaacc gcccgatgga ctgggactgg
     6781 attaaagcgt tcccgcaaac gctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6841 tgggaaaaag cctggcccgc cgtattcatc gccttcctcg ctggtttgcc gctgctgttg
     6901 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca aaaactggct
     6961 tccgctgtgg gttccctgcg taacgacagc cagctcaaca caccaaaagc gatccttatc
     7021 gacctgatcc gcgcactgcc ggtgtgcctg attattctcg ctgtcggcct gattctgctg
     7081 actatgcagc tcaatatcag cgagttattg tggtcgttca gcaaaaaact ggcgattttc
     7141 tggctggtgt ttggcctgtg ctggaaagtg ctggagaaaa atggtgttgc cgtgcgtcac
     7201 tttggtatgc cggaacagca gaccagccac tggcgtcggc aaattgtccg catcagcctc
     7261 gcgttgctgc cgatccattt ctggtctgtg gtggcagaac tctccccgct gcatctgatg
     7321 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggtg
     7381 tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7441 attaccgtgc tgtcgattat cccgattgcg ttgatggtgc tgaccgccac gggttacttc
     7501 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatttggt gatcatctgg
     7561 aacctgctgt accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7621 cgccgtgcac tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7681 ccggaagaac ccaccattgc gctggagcag gttaaccagc agacgctgcg tattaccatg
     7741 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtctga tttgatcacc
     7801 gtgttcagct atctcgacag cattacgctc tggcattaca acgctactga agctggtgct
     7861 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7921 gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag tgctggtgct ctcgcgactg
     7981 aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8041 gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt gggataaact ccagtggctg
     8101 gcggcagcat tatccgtagg tcttggtttt ggtttacaag agatcttcgg taacttcgtt
     8161 tccggtttga tcattctgtt cgaacgtccg gtgcgcattg gcgacacggt aaccattggt
     8221 agcttctcgg gtacggtcag caagatccgt attcgtgcga caacgattac cgatttcgat
     8281 cgcaaagaag tgattatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8341 ttgactgaca cgactacgcg tctggtgatc cgtctcggcg tagcctatgg ctccgatctg
     8401 gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8461 ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt
     8521 ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatt
     8581 gatcagttgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8641 cacaacgaga agggcgatga agtgacggaa gtgaaacgcg actacaaagg cgacgatccg
     8701 acgccagcgg tagggtaagg acaataaagg gcgacagtga tgccgccctt tattttttac
     8761 tcgctttgtg aagtctcatc gctctgtaac ttcttttcat aatcgcgttt tacaatatcc
     8821 agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg
     8881 acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc attgctagcg
     8941 ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcaac gtgccaggcg
     9001 cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat cggtcgctcg
     9061 cgccagacgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg
     9121 ctgaatacgt ttacgctgcc agcgggcaat tttcggtggt gcgctatccc actcgcgtag
     9181 cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat gttctgccag
     9241 ccaggcgact tgcggcagct gttgctgctc aaccgcatga cgaagcgcag ccaggttatc
     9301 gcccgactca tcgagacaag cctgtagtgt tgtcgcacgg gtctgaaaaa ggtgcctgtc
     9361 aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac gcagcgtggc
     9421 gagctgtcct tccagttttt ccagtagcag ggcggttttc acgataagac ctcaatgaaa
     9481 atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga
     9541 atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta
     9601 ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc
     9661 ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg
     9721 cagaaacatc atgcgatgcc gcgcggcgca aaaccacggg cgattttgct tattttgatt
     9781 acgtttacga tttcgctgtg gttcgtccag atgccgtggg tgcgcatcat gttgctggta
     9841 attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga aaagcaagaa
     9901 aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg
     9961 ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgttcaa
    10021 aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc
    10081 agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc
    10141 ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact
    10201 tgctggttga gcgttacaaa aatgcgggca ttaccaaagt ggtcggcacc gaagcgcgtg
    10261 gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta
    10321 aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg
    10381 atcaactgga aatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg
    10441 atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg
    10501 gtgaagtagc tgacgctgcg ttcattatca acctgttcga tctcggcggt gaacagcgtc
    10561 tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgt
    10621 cagtcttgcg ctgctcacgc tacgggcagc acaagatgtg cattcagcct cgccgttctg
    10681 acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc
    10741 aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg
    10801 ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc
    10861 ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa
    10921 ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg
    10981 tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgtaccaa
    11041 agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt
    11101 caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta acgcactgtt
    11161 aaaaaccctt gaagagccgc cagagcacgt taagtttttg ctggcgacga ccgatccgca
    11221 gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaagg cgctggatgt
    11281 cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc
    11341 gcgggcgctg caattgttgg cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct
    11401 gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggggg tcagtgcgat
    11461 gctgggtacg cttgacgacg atcaggcgtt atcgctggtt gaagcgatgg tcgaggccaa
    11521 cggcgagcgc gtaatggcgc taattaatga agctgctgcc cgtggtatcg agtgggaagc
    11581 gttgctggtg gaaatgctcg gtctgttgca tcgtattgcg atggtacaac tttcgcctgc
    11641 tgcacttggc aacgacatgg ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat
    11701 accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc
    11761 gtatgcgccg gaccgccgta tgggcgttga gatgacgctg ctgcgcgcac tggcgttcca
    11821 tccgcgtatg cctctgcctg atccagaagt gccacgccag tcctttgcgc ccgtcgcgcc
    11881 aacggcagta atgacgccaa cacaggtgcc gccgcaaccg caatcagcgc cgcagcaggc
    11941 accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca
    12001 gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg
    12061 gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg
    12121 cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc
    12181 gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc
    12241 gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag ccattgagcg
    12301 cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg aacaggtggc
    12361 gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc gctcctctca
    12421 gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga gtacgttcaa
    12481 aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc gtacgccgct
    12541 ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc
    12601 ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg aagaaagtat
    12661 ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga ttgagagaga
    12721 aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag cagatgcaag
    12781 aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc gaatctggcg
    12841 caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta gagatcgacc
    12901 cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca gcattcaacg
    12961 acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa
    13021 tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc tgttaacaca
    13081 gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc agcgtatggc
    13141 gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg
    13201 ggcgatgtcg gaaatcggcc actgcgccga ttgtcgcacc ttcaccgaac aggaagtctg
    13261 taacatctgt tcgaatccac gtcgtcagga aaacggtcaa atctgcgtgg tggagagtcc
    13321 ggcggacatt tacgccatcg agcagacggg gcagttttca ggtcgttatt ttgtgttgat
    13381 gggacacctg tcaccgctgg acggcatcgg tcctgatgat atcgggcttg atcgtctgga
    13441 acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc ccacggttga
    13501 aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg tggaagccag
    13561 ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg gcaccacgct
    13621 gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga gcaggatcac
    13681 ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct gtttttaacc
    13741 ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt ggttttcagt
    13801 cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat aaagaaatct
    13861 tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt ttccgcgcgc
    13921 tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc tctttcgata
    13981 aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc gacgaagtga
    14041 ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa tccctgggtt
    14101 ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc tactctgcgt
    14161 ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg
    14221 gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc actaaagaag
    14281 atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc ctcgatgact
    14341 ggcgtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg gtagagatcg
    14401 aaaaacgcga agagaaagac ggcgaaacca ttatctcctg ggagaaaatc aacaaagcgc
    14461 aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa gagttctaca
    14521 aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc gttgaaggta
    14581 agcaggagta caccagcctg ctgtacattc cgtctcaggc accgtgggat atgtggaacc
    14641 gcgatcataa acacggtctg aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag
    14701 aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc
    14761 cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac ctgcgcaatg
    14821 cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac gcggaaaaat
    14881 accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg gaagatttcg
    14941 ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tacccatacc gattcttctg
    15001 cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag gagaaaatct
    15061 actatatcac cgcagacagc tacgcggcag cgaagagcag cccgcacctg gaactgctgc
    15121 gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact
    15181 atctgactga gttcgacggt aaaccgttcc agtctgtgtc taaagttgac gagtcgctgg
    15241 aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca ctgactccgt
    15301 tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc
    15361 gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc actcagatgg
    15421 cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc ttcgaactga
    15481 acccggatca cgtactggtg aaacgtgcgg cggatactga agatgaagcg aagttcagcg
    15541 agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg ctggaagatc
    15601 cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat gccggatgac
    15661 cttcgtgtca tccggcattt ttcttttctt catctgcact ttccgcaaat tatcttgcca
    15721 ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg gtatcgttta
    15781 tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt atcattctgc
    15841 ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag aaatatggta
    15901 ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc tccgagctgg
    15961 gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa ctggtgatcg
    16021 cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg ttggacggct
    16081 tcccgcgtac cattccgcag gcagacgcaa tgaaagaagc gggcatcaat gttgattacg
    16141 ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc cgccgcgttc
    16201 acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta gaaggcaaag
    16261 acgacgttac cggtgaagag ctgactaccc gtaaagacga tcaggaagag accgtacgta
    16321 aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac tccaaagaag
    16381 cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg gctgaagttc
    16441 gcgctgatct ggaaaaaatc ctcggctaat caagccgcag cagatctgct ttcgggcagg
    16501 tctgctcatc ctcctcaggc aatacctctt acagcaatta gttcttcttc ttcacttttc
    16561 cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg
    16621 tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg taaaacgcta
    16681 tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt tatggtggcc
    16741 gttgctgcgc ggcgtgattt tgccgctgcg ttcgccgcgt gtggcgaagc tgtatgcctc
    16801 tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac agcaggcgct
    16861 ggcgcaacgt ttaccggaga cgcccgtggc gctgggaatg agctacggct cgccatcact
    16921 ggaaagcgcc gtagatgaac ttctggcaga gcatgtagat catattgtgg tgctgccgct
    16981 ttatccgcaa tactcctgtt caacggtcgg tgcggtatgg gatgaactgg cacgcattct
    17041 ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg ataaccacga
    17101 ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg gcgaaccgga
    17161 tctgctgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag gcgatgatta
    17221 cccgcaacgt tgccgcacaa caactcgcga actcgcttcc gcactggaga tggcaccgga
    17281 aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga tgccttatac
    17341 cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtgggt catattcagg tgatgtgccc
    17401 gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa accgtgaagt
    17461 cttcctcggc gctggcggga aaaaatacga atatattccg gcgcttaatg ctacaccgga
    17521 gcatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa
    17581 ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa
    17641 ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt
    17701 ctggtaaagc agatggctgt catccagcag cggatcgaac tccgccccgg caataaaaca
    17761 gggcggaact tcgcgagtga gatcattatt aaacaggcag taatatggcg actcgcggtc
    17821 cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg ttaagccatc
    17881 ccagacacca cccaacagac gacgagccac ggaatcccgt aatccgtaaa gcccatacca
    17941 cagcaaaacg cccgcaactt taccgcaatc gatctgttta tcgcgcaacc acaacgcact
    18001 ggcgagcgcc agcatggcac ctgcggaatc accggcaaaa ccaatgcggg acatattgat
    18061 ttgataatcc tccgcctgct ggtggaaata acaacaggca gccacaattt cctctatcgc
    18121 ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaatg ccaatcaccg tacattggct
    18181 gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga gaataaaacc
    18241 gcctccatgc aaataaaata gcgtcgccgg gctatctggc tgcggataaa agatacgtgt
    18301 tttcacctgc ccatattttg ttggaaccct gtaagctctg gttgccattt ctggagcgtc
    18361 cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa tcgctcccgt
    18421 tgcaggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca tttcagcaga
    18481 gataaggtcc agaacgggta atttgttttc cggcttcatg caaaaactcc tttcaaatta
    18541 cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg
    18601 ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt aaccacccgt
    18661 aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc ccgtaaacgc
    18721 acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgctg cctgggtagt
    18781 gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat tcattgagcg
    18841 ttatggatta agcgccgggc attcactggt gattgaggat gacgttgccg aagcgcttta
    18901 tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca ccattggtaa
    18961 caccatgcac aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg
    19021 cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacacct ccagccgtac
    19081 cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta cgctgattgg
    19141 cgagtccggg gaacgtacct ttgctatcag cccgggccac atgaaccagc tgcgggctga
    19201 aagtattccg gaagatgtga ttgccggagc ctcggcactg gttctcactt cttatctggt
    19261 gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagccattg agtacgcgaa
    19321 gaaatataac gtaccggtgg tgctgacgct gggcaccaag tttgtcattg ccgagaatcc
    19381 gcagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga acgaagatga
    19441 agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg cgctggactg
    19501 ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg gctttaccga
    19561 agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggggctatag cggaatttaa
    19621 tcagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc tgcgtgtcta
    19681 ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta atggagcagg
    19741 ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc accgtagcaa
    19801 cgtaccaaac tccagcaaac ataaattcac ctggttgacg tattcgtcat tagcgcaggt
    19861 gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac ctcgtttaac
    19921 gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc gttaagttat
    19981 cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcattttgc tctcaatgcc
    20041 tgatgcgacg ctggcgcgtc ttatcaggcc tacatatccg cgcctattat ccggcaacca
    20101 ccgtttacct cgtcaccacc tcacccgccg gtggcgtttc cagcagttcc agcatagtac
    20161 gggcaatttc acgctcaccc atcactacct gattcgcacc acgttcggtg atatacgcca
    20221 cttcatcgtc ataatgggcg cgggcaataa tctcaatatc cggatttttc gcgcgggcag
    20281 atgccacaat ctcgcccgct tcatagccgt tgggaatcgt caggatcagc cattttgcac
    20341 attccagatg cgccagttgc ataatttctt cgttagccgc attgcccaat actgcgcgga
    20401 ccccgcgctc gcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa
    20461 tatcagaggc gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccagta
    20521 gcgcatggtt gcaaatatcc actgggatct gcttctcttc ttcgattgcc tcttccagcg
    20581 tctgctcttc cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg
    20641 ggttgagcat aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca
    20701 gtaaattcaa tgccattcct agtcccgcca ggataaacgc gaactcacca atctgcgcca
    20761 ggctggcggc gatggttaac gccgtgcgct gggagtgacc aaacaaacgc acaaggaaca
    20821 gggccgcgac cgatttacca aacagaataa ttgccagcgt cgccagtact gccagcggtt
    20881 gctgaatcag aattaacgga tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa
    20941 acgcgtcgcg caatggcagt gtatcgtggg cggcacggtg actcagttca gactcgttca
    21001 gtaccatccc ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac
    21061 caaaggcaat ccctaacgcc agcgccagca ccgacagggt aaacagctcg cgggaaccgg
    21121 ttgctgcgct gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg
    21181 cgataaatgc gatcaccttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc
    21241 ccacatcgcc ctgttccatc attcctgcta ccgcgggtag caataccagt gtcaggacca
    21301 tcaccaggtc ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtcaatta
    21361 attgccgttc ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataagc
    21421 cgaacacgat accggtcatt aacgaccagc ccagcacggc ggagagcgcc atacccagca
    21481 gcgtcgccac ggctatctgg gcgatcgcgc cgggaatggc gatggacttt accgccatca
    21541 aatccttgag cgaaaaatgc aaaccgacgc cgaacatcag caaaataacg cccagttcag
    21601 ccagttccgg cgcaagctta gtatcggcaa caaagcccgg agtgaatggt cctgccagca
    21661 cacccgctaa cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga
    21721 ggataaaggc gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca
    21781 ttccgtctcc ttttcctggt ggttattgtc catttttggc cgggaaaacc aaaattacag
    21841 gtaatagttt atgacaattt cattgatgat gtttatgaat aattgttgaa ttttgcagaa
    21901 aaatggaatt agctgcaaaa aaaagcacgg atcggaaaac agaaggcggt ttaacagagg
    21961 aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct gcaatgaaat
    22021 cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag tggcaggaaa
    22081 gcacagattt tatagactaa ctctatgctg gtgtggtcgg cgataagccc cagaactgcc
    22141 gcccccagac ctcccatgcc gaaagcaaaa ccgaaaaata gtccagaaac cataccgata
    22201 cgtcccggaa gcagctcctg ggcatagacc agaatggcag agaatgccga agcgaggata
    22261 aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc
    22321 gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgtcc aattttatcc
    22381 cctacaggcc cgccaatcac cgtacctgcc gcaacggcaa acaggaaggc aaacaaatga
    22441 agctgagcat tctggataga taatccgaat ttttgcatca gataaaaggt gtaatagctg
    22501 ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat gctgaccgcc
    22561 agaacaactt tattgcgcgg cagtggattg ataatcgtcg ctttgggttt tcctttattc
    22621 attcggtgct gtgccgagta ccaacggctg atttgcgcca acaccacgat cgccagcagt
    22681 gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat caccgccgcc
    22741 agcaaggggc ccagggaact gccaaagttg ccgccgacct gaaatataga ttgcgccagg
    22801 ccatgacgcc cgccggaagc catacgggcc acgcgagaag attccggatg aaagaccgat
    22861 gaaccggtac cgaccagcgc cgctgccagc agaactgcgc cgaaactgcc cgccagcgcc
    22921 agcagcacca gaccacttaa ggtaaagcac atgccaatcg gcaacgacca cggcatcgga
    22981 tatttatcgg tccagtagcc gaccaccggt tgcagtagcg aagaggcgag ctggaaggtg
    23041 agggttatca tgccaatctg cataaatgtc agagaaaatt ctgactgaag cagtggataa
    23101 atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct gatagcacct
    23161 aaaataccaa acgatgttcg ggccttagtc gttgacgcag ccgcgcccgc cacaggctgg
    23221 gtttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat
    23281 cttatttgtg attattaccc gactaacata cctgtatgcg ccgcctgaag gaagtctcaa
    23341 cacggaatac agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtt
    23401 tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga gagaagtatg
    23461 aaattattgc agcggggcgt ggcgttagca ctgttaacca catttacact ggcgagtgaa
    23521 actgctctgg cgtacgagca ggataaaacc tacaaaatta cagttctgca caccaatgat
    23581 catcatgggc atttttggcg caatgaatat ggtgaatatg gtctggcggc gcaaaaaacg
    23641 ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc
    23701 ggtggcgaca ttaacactgg cgtgcccgag tccgacttac aggatgccga acctgatttt
    23761 cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataac
    23821 ccgctcaccg tattacgcca gcaggaaaag tgggctaagt tcccgttgct ttccgccaat
    23881 atctaccaga aaagtaccgg cgagcgcctg tttaaaccat gggcgctgtt taagcgtcag
    23941 gatctgaaaa ttgccgttat tggcctgacg acggatgaca cagccaaaat tggtaatccg
    24001 gaatatttca ctgatatcga atttcgtaag cccgctgatg aagcgaagct ggtgattcag
    24061 gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac
    24121 gacaatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct
    24181 gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg tatggcggca
    24241 gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccgga tcaacaaaac
    24301 ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgattttgag
    24361 tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag
    24421 aaagtgacct gggaagacgg gaaaagcgag cgcgtgctgt acacccctga aatcgctgaa
    24481 aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc gcagctggaa
    24541 gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta
    24601 cagaccaata tggggcggtt gattctggca gcacaaatgg atcgcactgg tgccgacttt
    24661 gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggtgatat cagctataaa
    24721 aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat gaccggtaaa
    24781 gaggtgattg attacctgac agccgtcgca caaatgaagc cagattcagg tgcctacccg
    24841 caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct gaaaatcaaa
    24901 ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga cattaaactt caatgccacc
    24961 ggcggtgatg gctatccgcg ccttgataac aaaccgggct atgtgaatac cggctttatt
    25021 gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt gagtgtttat
    25081 gaaccgaaag gtgaggtgag ctggcagtaa tgaaccccgt cgcggcgtca tgatatgacg
    25141 ccgccattta tgaatattga aatcttgcat cgtaatcgcg atgtgtcaaa ccaaatgcca
    25201 tcagcatata attctgcgga tcaccaggct gttcttcatc tgcttcaaac caaagtgtca
    25261 cgcctgagcg attgacgtgg attaagagtg tatggggcga ttgacggcgg atttgcagat
    25321 cagcgccttc gttaacaagc atgttaaata cacgcgtaac gttcaactca gcgacgttat
    25381 tcaatattcc tttatcaaat cggatcgctt tgcccaaatg gatttggctc agataatcgc
    25441 accaaatcat atatagcttg ttgttttcga aatgaaactc aacattacca aatagccaga
    25501 tataaaaacc atttcccata tcatttttag agtccggctc aacaaagttt tgttccaacc
    25561 atgccattgt ttgccccagt tcgacaaaat cgaattttcc gttaaagaca aaattgtgga
    25621 agctaacatc aacaagatgg tcaaatttca tctttcgctc ctttgtgaat tttttacatg
    25681 cttcaaaagc cagcgtaatt tacatcggtg caggcgactc tccagtgttg agcgaaaatt
    25741 tgcgatgtcg acattggacc gatctaccgc agcaaccccc gtgtattgtg ggatcggtga
    25801 agggattttt tgtaactttc ccagcgccag cagttgttga atgatcctgc taccgtcgtg
    25861 gtaatcatcg taaatagcca ctggcagagg ctgccaggcg ggggtcgatt tgataaatcc
    25921 ggaaaaaaaa tacgggtcca gatcgccgaa atcaaagccg taggcgaggg gaacttcaat
    25981 attttgctct gtttttttat tctcaaatgt acaatgaaaa ccgtgaaaac aatactgcca
    26041 ctttcccatt ttgccttttc gcttacggtt gcatttgaag ggggcaaagg taactagcgg
    26101 gttattttcc gccatctcaa cgttcagttg ttcagccaat gcaaatataa gctctgtcgc
    26161 cagcttacga tattctttcg cgcagtgttc gaaaaaatcc tgatggcggt taatctcttc
    26221 aagcgatatt aatctggcga tcctgataac cgggtcggca gaatgcatgc caacaacaaa
    26281 ttcatcgagc tgtgctttca gcaccagccc gaattcctca cgatacctct ctgcttcgct
    26341 ggcaaaatgc agatgtaata ccttctctct cgatgtttgc cgaagtaagg cgatatccgg
    26401 tatctcatct gcacaagcga tacttaatgt ctctgtaatc ctttccacat ctcatcttcc
    26461 gtgtgcatca cttaatcgcg acgggcgata tcagcaaatt tggcatcgag tatctttgcc
    26521 aggtcgcttg ccgccagttc gatatccagt ccgcgcttgc cgccggaaac ataaatagtg
    26581 gcaaattctt gtgcgggggc gtcgataatc gttggcagac gttttttctg ccccagtggg
    26641 ctaatccccc ctaccagata ccccgtcgaa cgctgcgcga ccatcggatc ggccatctca
    26701 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
    26761 gtaacagcca cggcaaggtg tttcatatca ccgttcactg cgaccagcag cgttttgtag
    26821 acctgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagca
    26881 ggatcgtgct cgtaggtgtg gatttgaaac gaaatcttgt ttttttcgag taatttaact
    26941 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
    27001 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
    27061 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
    27121 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatctgc ggcaaattcc
    27181 cttctccata caggtgtagt gcaccgaccg cgaccacata gcgccccggc ggcatggcgc
    27241 gtaatttgtc ccgccaggcg aggtttcgct gatgcatcag cacatcgtac agcgactgac
    27301 tgaacgtatt gggcagcgtt atatcattat tttgcggcgg tgcattcagc caccagctca
    27361 tcatttgttg cagcaaccgt gcgttggtgt gccagtgggt cagcgtatcg tccagcagcg
    27421 ccagtccttt gtcagggagc tggagcaaca tggcaatctg gttttcagcc ccttccagtt
    27481 caatcacggg tttatgttgt tgcttagccg cctgcaatag ctggtaatcg ataccgtatt
    27541 ctgcccgcag ccccagtttt tgcgcctgcg tcgcctgaag aaccatcgcg atttgccaca
    27601 gcggttgggt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcaggt
    27661 tttggagttg gtcctcgcta atgcgctctt ccagggcctc gcaggcaggc aaattggcaa
    27721 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
    27781 ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga atactgccga
    27841 tcagatgaaa atggcgattg ccgggaagag agatatcgat agcaggccag gtgtaatgat
    27901 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
    27961 ttgtgaaata tcatgctagc gcgcggggag ggatggcgca aatggagttg attgttgcac
    28021 ttgtgcacag tgcttgtgtg cattacaatg attaaataat ggacaaggag atgagagagg
    28081 aatggctcaa aagaaaaata taaggagttt tcgtgatgcc tggttggctg atttttttgt
    28141 ccattcaacg ccacatcgaa agatccctgc tgaaatccat accacgttat cacgaaagtt
    28201 ggacatcatt aatgctgcca cttctcatcg ggatttacgc tcccctccgg ggaaccgata
    28261 cgaagagtta tccgggaaat tacaggaata ctcctccatt agagtgaata aacagtaccg
    28321 actgattttt aaatgggtta atggcaaggc cgaagaattg tttttagacc cacacaacta
    28381 ctaaaacata gctgtcgtat tgacgagaaa ggtaaaacta tgaaacaggc aacaagaaaa
    28441 ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga tttgaaaatc
    28501 aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtgcact gatcaataac
    28561 aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga taccacagtc
    28621 gatttttggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa caacatgcgt
    28681 acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg ccgtgaagag
    28741 cgtgcaaaaa aggtcgcgta gaataatttc cctataacgg atgcgccacc ctaaagcagc
    28801 gcatccgcaa tgatgtactt attccttcgg tttaaaccgc agcaatcggt tggcgttact
    28861 cactacggtg atcgacgaga gcgccattgc cgctccggca accaccgggt taagcagtgt
    28921 tccagtgaac ggccacaaaa taccggcggc gaccggaata ccaatgctgt tgtagataaa
    28981 cgcaccgagc agattctgct tcatgttgcg cagcgttgcg cgggaaattg caagcgcatc
    29041 cgcaacaccc atcaggctat ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc
    29101 actgccgcca cccatcgcaa taccgacatc cgcctgagcc agcgctggcg cgtcgttaat
    29161 gccgtcgcct accattgcca cctgacgtcc ttcactttgc agacgtttga tcgcttcggc
    29221 tttaccgtct ggcagcacgc cggctatcac ctcatcaatc cctgcttctt tggcgatcgc
    29281 attggcggtg gtcgggttat ccccggttag catcaccagg cgatatcccg ctttatgcag
    29341 gcgttgcagc gccgccacgc tatcactacg caacggatcg cgtacagcca gcagggctac
    29401 cgctttcccg tcaaccgcca gcagcacagg cgttgcccct tgcgatgcct gagctgaaat
    29461 atctgcttca attgctttgg tatcgacctg ttggtcattt aacagcgcct gattgcccag
    29521 caataacgca tgaccttcag cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc
    29581 gttgacctgc ggtagctgca tatcacctgc tttatcgagg atcgcccgtg ccagagggtg
    29641 gctggaacct tgctccagcg ccgccgccag acgcaatgct tgcgcttcat caatatcagc
    29701 aaatgttttt actgcgacaa cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa
    29761 cactacggtg tcgagcgtac tggcgcgttg cagcgcgtcg gcgtcccgta ccagcacgcc
    29821 aaactcagcc gcccgcccga cgcccgaaat aatcgacatc ggcgtcgcca gccccagcgc
    29881 acacggacag gcaataatca gcaccgtggt ggcaatcacc agggtataga caatctgcgg
    29941 tgccggacca aagaaatacc agattgccgc actgatcagc gcaatcacca ccactaccgg
    30001 cacaaatacg gctgagattt tatccgccag ctgaccgatt tctggcttac tgctctgggc
    30061 ctggcgcacc atacgaataa tccgcgacag cgtagtatgg ctgccaaccg cactggcgcg
    30121 aaacagcacg ctaccgtcct gcaccactgt cccggcatgg acgctatcgc cttcgccttt
    30181 ttgctgcggg ataggttcgc ccgtcagcat cgcttcatcc agccatgctt cgccctgggt
    30241 aatctctcca tctaccggta cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg
    30301 cacttccgcc agtggcacgc ttttttcacc ttcgtcagta accaggcgcg ccgtcggcgg
    30361 agttaaatcg agcaatttct ccagcgcctt tgaagaacgc tgacgtgcgc gcgcttccag
    30421 catatgaccg agattaatca gaccgataat catcgcactg gcttcatagt aaagatgccg
    30481 cgcttccatc gggaaccact gcggccacag gttgacgctc atcgaataga gccacgccac
    30541 acccgttccc agcgccacca gcgtatccat cgtcgccgca ccgttcagca ggcttttcca
    30601 tgcgctgcgg taaaaatgcc cgccggcgaa aaccattacc gccagggtta tcaggccgat
    30661 aactaaccac aggctgcggt tgtcagcggt gaccatcatg ttatcgccga tcatccccca
    30721 gaccatcacc gggataccca ccgccagcgc gacaattgcc tgccagcgga agcgcttcat
    30781 cgtagcgacg gcggtttctt gctggcgctc gcggcgttta gcgtcatctt caatcgcttc
    30841 cgcgccgtag cccgcttttt ccaccgcctg cactaaatct tgtggggagg cactgcccat
    30901 caccagcgca gtacgctccg ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg
    30961 caacgcattt tgtacgcggg tgacacagct ggcgcagctc atgccgctca gcagcaactg
    31021 ctggctgtca tcgtcatcgg cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc
    31081 ttccgacggg attgatgact ccgccagcgg tttagccttt gggtggctta cagatgcgtc
    31141 ataacccgct tgtttgatgg tttcgattag ctgttctgca ctggcagtcc cggtaacgtg
    31201 cgcttcagtg atagacacat ccgcctgttc aacatccggg cgctgttcga gactttcttt
    31261 cacgcgttta acgcagtgac cgcaggacag gccgtccagg gtcaggtcga tagtttgtga
    31321 cataaaacac tcctttaaga cagttttgac tggctgtgat aaaggttaaa ccttccagca
    31381 aggggaaggt caagaaatta ataaaccagg cgggtaaaag tccgtaaaga ttaaaaaatc
    31441 ggctcgattt gcatcaggat tagacattta tctctttgtt ttcctgtagt taagttgcgg
    31501 gtgctaagtt aaatcaggat gcatgaaaat cggcaccggg gtgaggaatt acctcccgca
    31561 tctataaaaa ggagttaaca aaagatgtta gatgcaaaca aattacagca ggcagtggat
    31621 caggcttaca cccaatttca ctcacttaac ggcggacaaa atgccgatta cattcccttt
    31681 ctggcgaatg tccctggtca actggcggca gtggctatcg tgaccagcga tggcaacgtc
    31741 tatagtgcgg gtgacagtga ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg
    31801 ttagcccttg cgttagaaga tgtcggcccg caggcggtac aggacaaagt tggcgctgac
    31861 ccgaccggat tgccctttaa ctcagttatc gccttagagt tgcatggcgg caaaccgctt
    31921 tcgccactgg taaatgctgg cgctattgcc accaccagcc tgattaacgc tgaaaatact
    31981 gaacaacgct ggcagcgaat tttacatatc caacaacaac tggctggtga gcaggtagcg
    32041 ctctctgatg aagtcaacca gtcggaacaa acaaccaact tccataaccg ggccattgcc
    32101 tggctgctgt actcagccgg atatctctat tgtgatgcaa tggaagcctg tgacgtgtac
    32161 acccgtcagt gctccacgct catcaatact attgaactgg caacgcttgg cgcgacgctg
    32221 gcggcgggtg gtgtgaatcc gctgacgcat aaacgcgttc ttcaggccga caatgtgccg
    32281 tacattctgg ccgaaatgat gatggaaggg ctgtatggtc gctccggtga ctgggcgtat
    32341 cgcgttggtt taccgggcaa aagcggtgta ggtggcggta ttctggcggt cgttcctggc
    32401 gtgatgggaa ttgccgcgtt ttcaccacca ctggacgaag aaggcaacag tgttcgcggt
    32461 caaaaaatgg tggcatcggt cgctaagcaa ctcggctata acgtgtttaa gggctgatca
    32521 tgatgaacac ggaaggtaat aacggtaaca aacccctcgg tctatggaac gtcgtttcca
    32581 tcggcattgg ggcaatggtg ggggcgggga tcttcgcgct gctggggcag gctgcgttgc
    32641 taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg
    32701 gttatgccta tgcgcgtctg ggggcgagct atcccagcaa tggcggtatt atcgacttct
    32761 ttcgtcgcgg attaggcaac ggcgtttttt cgctggcgct ctcgttattg tacctgttga
    32821 cgctggcggt gagtatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt
    32881 tgcatgaagg cagtcaggag gagcacctta ttttgctcta cgcattgggg atcattgcgg
    32941 tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg
    33001 tcggcattaa aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac
    33061 cggcgcatat ttccgtttct gcgcccccca gctccggggc gttcttctcc tgtatcggga
    33121 taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag
    33181 atccggaggt cataatgcca cgggcgtttc tggtggcgat tggcgttacc acgttgcttt
    33241 atatctcgct ggcgctggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg
    33301 ccgataccgc cgtagcgcag gctgcttctc cgctcctcgg gcatgtgggg tatgtgatcg
    33361 tcgtcatcgg cgctttactg gcgacggctt cagccatcaa cgcaaacctg ttcgccgtgt
    33421 ttaacatcat ggacaacatg ggtagcgaac gcgaactgcc gaagctaatg aataaacctc
    33481 tgtggcggca gagtacctgg ggtaacatca tcgtcgtggt gttgattatg ctgatgacgg
    33541 cggcgctgaa tttaggctca ctcgccagcg ttgccagtgc cacctttttg atctgctatc
    33601 tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc
    33661 ttatcgttgg tacgttggtt atgttgttgg tgatcgttgg ctttatctac agtctctggt
    33721 cccagggcag ccgtgcgctg atatggatta ttggcgcact cttactcagc cttattgtgg
    33781 caatggtcat gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acctcctgta
    33841 ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa
    33901 accctttaac aaagcacagg aggcgttgcg cgaacgatga acattagcga tgtagcaaaa
    33961 attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg
    34021 ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc
    34081 ttactgcgcc aggcacgcca ggtgggcttt aacctggaag agagcggcga actggtgaat
    34141 ctgtttaacg atccgcagcg tcacagcgcc gacgtcaaac gtcgtacgct ggagaaagta
    34201 gcggagatcg aacagcatat cgaagaactg caatccatgc gtaaccagct gctggcgctg
    34261 gcgaatgcct gccccggtga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc
    34321 tgctgtcatc atcgggcggg gtgattaagg cttgcgaaaa tagtcgtcca ttattttcgt
    34381 tagctccgga cgttccccgt catcattgat ggcaaaatcc agaaaaaaag catctgcttg
    34441 cggcgttcgg ttttgcgcca tcaaccggaa ggtgaggggg cctgaccagg tttcggatgg
    34501 gctgatgtgc ttatcagggc cgaaactctc ccatgcgctg ggcggtaatg ttttgcacgc
    34561 ggtgagtttt ggtatgtggt attgcagctc tgttttgtgc agacattccc cgccaggaaa
    34621 taccagggtt tgttgctcat cattaaaatg ggttgccgtg ctgagcacca gtgttttgag
    34681 cggtcgccct tccagcgcgg cggcagtagc cttatggtgt ccgtcgagta gaacgcagag
    34741 ataggaatca gggagatacc aggccagacc acgggcaatt ccagttttcg agagcagaca
    34801 aaaaccgggc ggctccctgt tcatcctccg caaaaccgct gtagacaaat tccgcacagg
    34861 tagggcatcc agcctcggac cagaaacgta ttaacgggac atcctgccag cagagcgaat
    34921 ggataagacc gtgcgagtgc ggatcgtaaa tcacacattg acgcagaaat cctttcccgg
    34981 acttcaccga aaaaatgacg cccgcagcgg tatttttcag cagacgtact tcaggttttc
    35041 gaaaggggga aacatggcta ctccttagcc gaacattcaa ttaacactat gtggggggct
    35101 atatgccgga tgcggcgtaa acgccttatc cggcatgggt tttggtacag atttccaggt
    35161 gtgataagac gcgtcaggcg atgaccgcac ggataatcag cgttattcct tctatcgcaa
    35221 tcacctcaac atgcgtacca gcgccgagat cctcgctggc gctgacaggc catgaactgt
    35281 caccgacgcg catatgaccg cgcccgttga ccagcggaga ttccagaaca aaacgtcggc
    35341 caatcaactg ctgcccgcgc tggtttaaat gactgtcgct gtgcttttgt tcgcgcaccc
    35401 gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggacg gcaaacatta
    35461 ccccttgcca ctcccaaccc agcggcagca accagaccac cagaccggta atcaccgctg
    35521 ccacgccgct ccacaacaga taaccattgc cgcccaacat ctcggctgcc agcagcaaac
    35581 cgccgagact tagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt
    35641 ccgtttgttg gcgctgtctt tcaccagctc agcaatcccg gcaatcgacc ccatcaggct
    35701 gctggcctct aatggcatca tcaccacttt gctgttactg gacgaaccga tctgctgtaa
    35761 cgcttcggtg tatttctgtg cgacgaagta gttcaccgcc tgaatatcac cggaggcgat
    35821 ggcttcagac accattttgg tggcgcgggc ttctgcttca gcggaacgtt cacgcgcttc
    35881 agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgtg actgtttttc
    35941 accttcggct ttgaggattt ccgcctgacg gatcccttcc gcttcaagga tgtaagcgcg
    36001 tttggtacgt tccgctttca tctgcgcgtt cattgaagag ataagctctg cgggcgggcg
    36061 cacatcacga atttcgatac gggtgacttt aatcccccac gggttggtgg cctcatcgac
    36121 aatatgcagc agacgcgagt taatgctgtc gcgctgagag agcatttcat caagttccat
    36181 tgaacccagc acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt
    36241 gctgacttca taagccgcgc gcggcgcgtc aatcacctga ataaagcaca cagcgtcgat
    36301 ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatgtcga gcacttgctc
    36361 catcatattg atcttgcgac caatgcgatc cataaacggc accaccaggc ttagacctgg
    36421 ctgtaacgtt ttggtatagc gaccaaagcg ttctaccgtc cattgatagc cctgcggcac
    36481 gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat
    36541 aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa
    36601 acaaattata cctgattact gaaagagagt tcccccttat tcctgcgaag gataaactgt
    36661 ttttagtaaa aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct
    36721 acaaaacgta ggatatctgg cgggtgatac gaagattctt aataacatca atttttcgct
    36781 gcgtgctggc gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacactgct
    36841 aaaaatagtt gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga
    36901 tgtcagcaca ctaaagccag aaatctaccg tcagcaagtc tcttactgcg cccagacgcc
    36961 gacgctgttt ggcgatacgg tatacgataa cctgattttt ccctggcaga tccgtaacca
    37021 gcagcctgac ccggccattt ttctcgattt tcttgaacgc ttcgctttac cggacagcat
    37081 tttgacgaag aatatcgccg agctatctgg tggtgaaaaa caacgcatct cattgattcg
    37141 taacctgcaa tttatgccga aggttttatt gctcgacgaa atcaccagtg cgctggatga
    37201 aagtaataaa cataacgtta atgagatgat ccaccgttat gtacgcgagc aaaatattgc
    37261 tgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataaag tgattacact
    37321 gcaaccgcat gctggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg
    37381 aatcattagc actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa
    37441 aactggcgct ggagaaagat attctctgga gcgtcgggcg cgcgataatc cagctgatta
    37501 ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga
    37561 tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg
    37621 ctaaagcttt tatctcatcg tttattgcca ttacggtcgg ggcgggaatt accctggcgg
    37681 tactgatcct ctctgggtcg attgaattta tcccgatgca ggtgatccct atcgccggga
    37741 tgatagccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg
    37801 tcattagcga acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaaacagg
    37861 cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag
    37921 caaaaacggt aggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga
    37981 ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaaccg
    38041 ccagcttgtc gaccattatt gcctgctatt taacctatcg taagttttac aattcgcgcc
    38101 accaattggt ggtgacgcaa ttgaagaaga aatgatgcga tgccggatgc gtcttatcag
    38161 acctacatgg cacctgcaat gtattgaatt agcacggttt ggtaggccgg ataaggcgtt
    38221 cacgccgcat ccggcggcta tgcgcggatg cctgatgcga cgctggcgcg tcttatcagg
    38281 cctacaaacc agcacagcat cgcatttcaa cggaaaatca atacaacaac gcatacagct
    38341 ggcggcgata cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg atctcctgga
    38401 atgttttacg tgtctgacca tctgcggcgg tgagatcttt acgcagatgc ccaaacagta
    38461 actccagcgc ctcttcattg cgcccaacct ggtgcagttg cagtgccagt tgcgtcgcca
    38521 gtgcggcatc ttctggattc tccgccacct gctgttgcaa ctgttgaatt tccggcgtat
    38581 cagccgcctg cttcagcagt tcgatttgcg ccaccagccc ctgatagcgg gtgtcctgat
    38641 cctgcaacgg aatggttttc agcacggctt ctgcatcttc agaacggttc agcgcaatca
    38701 gcgtttctgc caacagcagg ccgatttccc cgttctgatt cgacaactgc caggcgtctt
    38761 tcagcaatgg cagggcgtcg gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg
    38821 ctttcagctc ttcttcacgc ggcagcactt tatccagcag gacgcggatc gcctcttccg
    38881 gttgcggccc ctggaagccg tctactggtt gcccgttctg gaacagatat acggtcggaa
    38941 tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca
    39001 gaataaattg cccgttgtac tgcgccgcga ggctttccag aattggggtt aactgcaaac
    39061 agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa
    39121 gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc
    39181 tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca actcacccct
    39241 gcaatatttt gtccatcacg cgccccggca gcaggcgctt aagcaccatt accgccctgg
    39301 tcaccagcgt caccggatag cgcatcttcg gcttctcgct aataaaagca tggcgcactt
    39361 tgccgaccac cgcttccggt cccaacgtaa agcgggcggc gatgccagga ttttcgactg
    39421 gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccaggtt
    39481 cgatcaggct gactttaatt ccgctgtggc gcagctccat gcgcagtgcg tctgaccacg
    39541 cctccagcgc atatttactg gccgcgtaag cgccacgacc cggtgtggag attaatccca
    39601 tcaccgatga ggtcatcaca atacgccctt cacggtgcgg caacatcgcg ggtaacaggc
    39661 gcatggtgag ctggtgtgcg ccgaaaaagt tagcggaaaa ctgctgttcc atctgcgcac
    39721 ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca
    39781 gacaattatc ggtcagggcg atcacttcgt cggctgcgcg atcaacactt tctggcgaat
    39841 ccagatcgat caacacgccg gtaaatccca tgctgttcat gcgctcaaca tcatccggtt
    39901 tccggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagtgcg ctttccaggc
    39961 caataccact ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga
    40021 tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg
    40081 gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc
    40141 ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt
    40201 ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt
    40261 atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc
    40321 gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccgt cattgccgcc cagttcaacc
    40381 agcacccagc gcggctgatg ctgtttcagc agagccggaa ggcgcgccag cccttgttgc
    40441 gaggtgtcac cgctgatgct ggcattgact accgacgttt tactctgcca cttatcattc
    40501 aacaaggcag gccaggccgc gctggcagac attcgatacc cggcgctcag gctatcaccc
    40561 agaatcaata acgtgtccgc tgcggcggca cggaaggtta acaggaccag gaacaggaag
    40621 ggcaaatgcc agcggaaaac attgttgaag ttcatcatct taagaagtcc gtcggtcagg
    40681 gggagcatga actctccatc ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca
    40741 tcgcactggt gggcgagtcg ggatcgggta agtcaacctt gctggcgatc ctcgccgggc
    40801 tggatgacgg cagcagtggc gaagtgagtc tggtgggaca accgctacat aatatggacg
    40861 aagaagcgcg ggcaaagttg cgcgcgaagc acgtaggctt tgtttttcag tcatttatgt
    40921 taattcctac ccttaacgcg ctggaaaacg tcgagcttcc ggcgctgctg cgcggtgaga
    40981 gtagcgcaga aagtcgtaac ggggcgaaag cgttgctcga acagttaggg ctgggtaaac
    41041 gtcttgatca tcttccggca cagctttctg gcggcgaaca gcaacgagtg gcgctggcgc
    41101 gagcctttaa tggtcgacct gatgtgctgt ttgccgacga acccaccggt aaccttgacc
    41161 gccagacggg cgataaaatt gccgatctgc ttttttccct caaccgtgag catggcacca
    41221 cgttgattat ggtgacccac gacctgcaac tggcggcacg ctgtgaccgc tgcttacggc
    41281 tggtgaacgg gcagttgcag gaggaagcat gattgcacgt tggttctggc gcgaatggcg
    41341 ctcgccgtcg ctattaattg tctggctggc gcttagcctg gcggtggcct gcgtgctggc
    41401 gctgggcaat atcagcgatc gcatggagaa gggcttaagc cagcaaagcc gtgagtttat
    41461 ggcgggcgat cgggcgttgc gcagttcgcg cgaagtgccg caagcatggc tggaggaagc
    41521 gcaaaagcgc ggcctgaaag ttggcaagca gctgactttc gccaccatga cctttgcagg
    41581 cgacacgccg caactggcga acgtcaaagc ggtggatgat atctacccga tgtatggcga
    41641 tctgcaaact aatccccctg gcctgaaacc gcaggcgggc agcgtattgc tggccccacg
    41701 cctgatggca ctgcttaacc tgaaaacggg cgataccatt gacgtgggcg atgccacctt
    41761 gcggattgcc ggagaagtga ttcaggaacc ggattccggt tttaatcctt tccagatagc
    41821 cccacgcctg atgatgaatc tggcggatgt tgataaaacc ggagccgtgc agccggggag
    41881 tcgggtcacc tggcgttata aattcggcgg cagcgagaac cagctcgacg gctatgagaa
    41941 atggttgtta ccccagctta aacccgaaca acgctggtac ggtctggaac aggacgaagg
    42001 cgcgctgggg cgatcgatag aacgttcgca acagttcctg ctgctttcgg cgcttctgac
    42061 cttgctgctg gcggtggcgg cggtcgcggt ggcgatgaat cattactgtc gcagtcgcta
    42121 cgatctggtg gcgatcctca agacgctggg ggcagggcga gcgcaactgc gtaagttaat
    42181 cgtcggtcag tggttgatgg tgctggtact ttcagccgtt accggtgggg cgataggcct
    42241 gttgttcgaa aacgtgttga tggtgctgct caagcccgtt ctgcctgctg cactaccgcc
    42301 agccagcctc tggccgtggc tgtgggcgct cggcaccatg acggttattt cgctgctggt
    42361 ggggctacga ccataccgcc tgttgttggc aacgcagcct ttacgcgtat tacgtaatga
    42421 tgtggtagcg aacgtctggc cgctgaagtt ttatctgccg attgtcaccg tggtggttgt
    42481 gctactgctc gccgggctga tgggcggcag tatgctgctc tgggcggtgc tggcgggcgc
    42541 ggtagtactg gctttgctgt gcggtgtcct gggctggatg ctgctgaatg tacttcgccg
    42601 catgacgctg aaatcgctac ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg
    42661 ggcgacgttg agccagcttt cggcattttc gctctccttt atgctgctgg cactgttgct
    42721 ggtgttgcgt ggcgatctgc tcgaccgctg gcaacagcag ctaccgccag aaagtcctaa
    42781 ctacttttta atcaacatcg ctacggaaca ggttacgccg ctgaaagcgt ttctcgcgga
    42841 acatcagata gtcccggaat cgttttatcc ggtggtgcgg gcgcggttga cggcgattaa
    42901 cgacaagccg acagaaggca atggagatga ggcgctcaac cgcgagctga atctcacctg
    42961 gcaaaatacg cggcctgatc ataacccgat cgtcgccggt aactggccgc caaaagccgg
    43021 tgaagtgtcg atggaagagg ggctggcgaa acgcttaaac gttgccctcg gcgataccgt
    43081 gacttttatg ggcgataccc aggagttccg cgctaaagtg accagcctgc gcaaagtgga
    43141 ctgggaaagt ctgcggccta atttctattt tattttcccg gaaggggcat tagacgggca
    43201 accgcagagc tggcttacca gtttccgctg ggagaatggc aacggcatgt tgacgcaact
    43261 caaccgccag ttcccgacca ttagcctgtt agacattggc gcgattttaa aacaggtcgg
    43321 tcaggtgctg gagcaggtaa gtcgggcgct ggaagtgatg gtggtgctgg tcaccgcctg
    43381 cggtatgttg ctgttgctgg cacaggtgca ggtgggaatg cgtcagcgtc atcaggagct
    43441 ggtggtgtgg cgcactctcg gtgcgggcaa aaaactgctg cgcacgacat tgtggtgtga
    43501 gttcgccatg ctcgggtttg tatccggcct ggtggccgca attggtgcgg aaacggcact
    43561 ggcggtactg caatcgaaag tgtttgattt cccgtgggag ccagactggc gattgtggat
    43621 tgtgctgccg tgcagcggtg cgctactgct gtcgctttgc ggcggctggc tgggtgcgcg
    43681 actgcttaag ggtaaggcgc tattcaggca gtttgtgggg tgatgtgata aagccgttta
    43741 tttctacctg gtagataaac ggctttatta ttagtgcatc acgtaa
//
LOCUS       DACADA010000023        40726 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.023, whole genome shotgun sequence.
ACCESSION   DACADA010000023 DACADA010000000
VERSION     DACADA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.023"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            62..826
                     /locus_tag="ISB17_001219"
     CDS             62..826
                     /locus_tag="ISB17_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979172.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(865..1791)
                     /locus_tag="ISB17_001220"
     CDS             complement(865..1791)
                     /locus_tag="ISB17_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1979173.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1881..2879
                     /locus_tag="ISB17_001221"
     CDS             1881..2879
                     /locus_tag="ISB17_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAP1979174.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2876..3094)
                     /locus_tag="ISB17_001222"
     CDS             complement(2876..3094)
                     /locus_tag="ISB17_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HAP1979175.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(3096..5111)
                     /gene="ligA"
                     /locus_tag="ISB17_001223"
     CDS             complement(3096..5111)
                     /gene="ligA"
                     /locus_tag="ISB17_001223"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP1979176.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5182..6180)
                     /gene="zipA"
                     /locus_tag="ISB17_001224"
     CDS             complement(5182..6180)
                     /gene="zipA"
                     /locus_tag="ISB17_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAP1979177.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6410..7171
                     /gene="cysZ"
                     /locus_tag="ISB17_001225"
     CDS             6410..7171
                     /gene="cysZ"
                     /locus_tag="ISB17_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAP1979178.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7356..8327
                     /gene="cysK"
                     /locus_tag="ISB17_001226"
     CDS             7356..8327
                     /gene="cysK"
                     /locus_tag="ISB17_001226"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP1979179.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8711..8968
                     /gene="ptsH"
                     /locus_tag="ISB17_001227"
     CDS             8711..8968
                     /gene="ptsH"
                     /locus_tag="ISB17_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAP1979180.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9013..10740
                     /gene="ptsI"
                     /locus_tag="ISB17_001228"
     CDS             9013..10740
                     /gene="ptsI"
                     /locus_tag="ISB17_001228"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAP1979181.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10781..11290
                     /gene="crr"
                     /locus_tag="ISB17_001229"
     CDS             10781..11290
                     /gene="crr"
                     /locus_tag="ISB17_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP1979182.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11333..12184)
                     /gene="pdxK"
                     /locus_tag="ISB17_001230"
                     /gene_synonym="thiJ"
     CDS             complement(11333..12184)
                     /gene="pdxK"
                     /locus_tag="ISB17_001230"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAP1979183.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12289..12657
                     /locus_tag="ISB17_001231"
     CDS             12289..12657
                     /locus_tag="ISB17_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAP1979184.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12660..13571)
                     /gene="cysM"
                     /locus_tag="ISB17_001232"
     CDS             complement(12660..13571)
                     /gene="cysM"
                     /locus_tag="ISB17_001232"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAP1979185.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13706..14803)
                     /gene="cysA"
                     /locus_tag="ISB17_001233"
     CDS             complement(13706..14803)
                     /gene="cysA"
                     /locus_tag="ISB17_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAP1979186.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14793..15668)
                     /gene="cysW"
                     /locus_tag="ISB17_001234"
     CDS             complement(14793..15668)
                     /gene="cysW"
                     /locus_tag="ISB17_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAP1979187.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15668..16501)
                     /gene="cysT"
                     /locus_tag="ISB17_001235"
     CDS             complement(15668..16501)
                     /gene="cysT"
                     /locus_tag="ISB17_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAP1979188.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16501..17517)
                     /locus_tag="ISB17_001236"
     CDS             complement(16501..17517)
                     /locus_tag="ISB17_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1979189.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17675..18466)
                     /gene="ucpA"
                     /locus_tag="ISB17_001237"
     CDS             complement(17675..18466)
                     /gene="ucpA"
                     /locus_tag="ISB17_001237"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAP1979190.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18746..19642
                     /gene="murQ"
                     /locus_tag="ISB17_001238"
     CDS             18746..19642
                     /gene="murQ"
                     /locus_tag="ISB17_001238"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP1979191.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            19646..21070
                     /gene="murP"
                     /locus_tag="ISB17_001239"
     CDS             19646..21070
                     /gene="murP"
                     /locus_tag="ISB17_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAP1979192.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(21248..22147)
                     /gene="yfeX"
                     /locus_tag="ISB17_001240"
     CDS             complement(21248..22147)
                     /gene="yfeX"
                     /locus_tag="ISB17_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAP1979193.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22243..22818)
                     /locus_tag="ISB17_001241"
     CDS             complement(22243..22818)
                     /locus_tag="ISB17_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAP1979194.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22879..23328)
                     /locus_tag="ISB17_001242"
     CDS             complement(22879..23328)
                     /locus_tag="ISB17_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAP1979195.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23315..23740)
                     /locus_tag="ISB17_001243"
     CDS             complement(23315..23740)
                     /locus_tag="ISB17_001243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAP1979196.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            23954..24823
                     /gene="amiA"
                     /locus_tag="ISB17_001244"
     CDS             23954..24823
                     /gene="amiA"
                     /locus_tag="ISB17_001244"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAP1979197.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24827..25726
                     /gene="hemF"
                     /locus_tag="ISB17_001245"
     CDS             24827..25726
                     /gene="hemF"
                     /locus_tag="ISB17_001245"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAP1979198.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25732..26784)
                     /gene="eutR"
                     /locus_tag="ISB17_001246"
     CDS             complement(25732..26784)
                     /gene="eutR"
                     /locus_tag="ISB17_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAP1979199.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26830..27330)
                     /gene="eutK"
                     /locus_tag="ISB17_001247"
     CDS             complement(26830..27330)
                     /gene="eutK"
                     /locus_tag="ISB17_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAP1979200.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27343..28002)
                     /gene="eutL"
                     /locus_tag="ISB17_001248"
     CDS             complement(27343..28002)
                     /gene="eutL"
                     /locus_tag="ISB17_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAP1979201.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28012..28899)
                     /locus_tag="ISB17_001249"
     CDS             complement(28012..28899)
                     /locus_tag="ISB17_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAP1979202.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(28920..30281)
                     /locus_tag="ISB17_001250"
     CDS             complement(28920..30281)
                     /locus_tag="ISB17_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAP1979203.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCILAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30293..31696)
                     /gene="eutA"
                     /locus_tag="ISB17_001251"
     CDS             complement(30293..31696)
                     /gene="eutA"
                     /locus_tag="ISB17_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAP1979204.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31693..32919)
                     /gene="eutH"
                     /locus_tag="ISB17_001252"
     CDS             complement(31693..32919)
                     /gene="eutH"
                     /locus_tag="ISB17_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAP1979205.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(33136..34323)
                     /gene="eutG"
                     /locus_tag="ISB17_001253"
     CDS             complement(33136..34323)
                     /gene="eutG"
                     /locus_tag="ISB17_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAP1979206.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34313..35149)
                     /gene="eutJ"
                     /locus_tag="ISB17_001254"
     CDS             complement(34313..35149)
                     /gene="eutJ"
                     /locus_tag="ISB17_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAP1979207.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(35160..36563)
                     /locus_tag="ISB17_001255"
     CDS             complement(35160..36563)
                     /locus_tag="ISB17_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAP1979208.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGPTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36575..36862)
                     /gene="eutN"
                     /locus_tag="ISB17_001256"
     CDS             complement(36575..36862)
                     /gene="eutN"
                     /locus_tag="ISB17_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAP1979209.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(36969..37262)
                     /gene="eutM"
                     /locus_tag="ISB17_001257"
     CDS             complement(36969..37262)
                     /gene="eutM"
                     /locus_tag="ISB17_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAP1979210.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37301..38317)
                     /gene="pta"
                     /locus_tag="ISB17_001258"
     CDS             complement(37301..38317)
                     /gene="pta"
                     /locus_tag="ISB17_001258"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP1979211.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(38314..39117)
                     /gene="eutT"
                     /locus_tag="ISB17_001259"
     CDS             complement(38314..39117)
                     /gene="eutT"
                     /locus_tag="ISB17_001259"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAP1979212.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(39114..39815)
                     /gene="eutQ"
                     /locus_tag="ISB17_001260"
     CDS             complement(39114..39815)
                     /gene="eutQ"
                     /locus_tag="ISB17_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAP1979213.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(39790..40269)
                     /gene="eutP"
                     /locus_tag="ISB17_001261"
     CDS             complement(39790..40269)
                     /gene="eutP"
                     /locus_tag="ISB17_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAP1979214.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40282..40617)
                     /gene="eutS"
                     /locus_tag="ISB17_001262"
     CDS             complement(40282..40617)
                     /gene="eutS"
                     /locus_tag="ISB17_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAP1979215.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9383 a        11421 c        10428 g         9494 t
ORIGIN      
        1 cttttaatca acctttttat taagcaatca actaataacg atcggcatag ggaattatgc
       61 tatgaaaaaa catcttttta ctctgacact ctcttctgta ttggcgatac cagcagtatc
      121 ccatgcagaa tttaaaggcg gttttgctga tataggtatt cattatcttg actggactag
      181 cagaaccact gagaaatcgt cgaccaaatc acacaaagat gattttggat atctggaact
      241 ggaaggcggc gctaacttta gttggggaga aatgtacggg ttcttcgact gggaaaactt
      301 ttataatgat cgtcatgata aaccgggtag cgagcagcgt tatacattta aaaataccaa
      361 ccgtatttac ctgggtgaca ccggatttaa tctctacttg catgcgtatg gcacctacgg
      421 ttctgcgaat cgtgtaaatt tccatgacga tatgttcctt tacgggatcg gctacaattt
      481 caccggcagc ggttggtggt tcaaaccgtt ctttgcaaaa cgctatacag accaaaccta
      541 ttataccggc gataacggtt atgtcgccgg ttgggttgca ggttacagct ttatgctggg
      601 cagtgagaaa ttcaccctca ccaactggaa cgagtacgag tttgatcgtg atgccaccta
      661 cgcggcaggt aatggcggca aagaaggtct gaacggtgcc gtcgcacttt ggtggaatgc
      721 aacttcacac atcaccacag ggattcagta tcgttatgcg gatgacaaac tgggcgaaga
      781 tttttaccag gatgcgataa tctactccat caaatttaat ttctgattga ttactgccgg
      841 agctcccggc agtatgaatt cactctatat ctgatacaac ggatcccctt cccgcccagc
      901 cattagcgca ttgccctggt ctcgtactac gtcccataac gcttcagcgg cggtggaaag
      961 tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca accactggcg taatgcgctt
     1021 taccaccagt gggctacctt caggcaatgg tagcgccagt gcgggaagaa tactgatgcc
     1081 gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt ccaatctcct gcacaatatt
     1141 cgcctgaata ccgttgcgcg ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta
     1201 atcctgcaac accaatttcg ctccctgtaa tgcctgccag gggacataat cttccacggc
     1261 caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg
     1321 cagatcgccc actgggccag gatcgatgac gatgccaaaa tcgacatctc cctggcgaat
     1381 actttccatc acccactgct gcggacgatc gtgcaggaca aactgaatat ctggatagcg
     1441 gcgatggctt tcagcaatgc attgcggaat aagatgcgcg gaaatggtct ggctggcagc
     1501 gacccgaact tttccgctca gttgttgccc catacgcccg gtatcgcgca acgtgctgtt
     1561 aagttcatcc agcagtcgct caagacgcaa agccaactgc tgccctgcat ctgtaagcac
     1621 cacttcacgc gtggttctgt ccagcaggcg aacaccagta tgattttcca gctccttcac
     1681 actgtgactc actgccgact ggctcaggcc gatacgctct cctgcacgac taaaactttt
     1741 ctcctgcgct actgtgacga aaacctttaa ttgctttaaa gaataattca tctgtttttt
     1801 tcatgaatgg atgcaataaa tcaattttat ttctcaaact ggaagacgca caatagaacc
     1861 atcgatcatc tggagtcttt atgaaacttt ttcgtatcct cgatcctttc accttaaccc
     1921 tgatcacggt ggtgttgctg gcctctttct ttccggccag aggcgatttc gtccccttct
     1981 ttgaaaatct gaccaccgca gcgatagccc tgctgttttt tatgcacggc gcgaagttat
     2041 cgcgtgaggc gattattgct ggcggtggcc actggcgact gcatttgtgg gtaatgtgca
     2101 gcaccttcgt gctgtttccg attctgggcg tgctgtttgc ctggtggaaa ccggtaaatg
     2161 tcgacccgat gctctactcc ggttttctct acttgtgcat tctccccgcg accgtgcagt
     2221 ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt
     2281 cagcatccag cctgctgggg attttccttt caccattgct ggttggtctg gtgatgaatg
     2341 ttcacggtgc agggggcagc cttgagcagg tcggtaaaat tatgctgcaa ctgttgctgc
     2401 catttgtgtt ggggcatctt tcccggccgt ggattggtga ctgggtgtcg cgtaataaaa
     2461 aatggattgc gaaaactgac cagacgtcca ttctgttggt agtttatacg gcattcagcg
     2521 aagccgtcgt taacggtatc tggcacaaag ttggctgggg atcattgctg tttatcgtgg
     2581 tggtcagttg cgttcttctg gctatcgtga ttgtagttaa cgtctttatg gcacgccgac
     2641 tgggcttcaa taaggcagat gaaattacta tcgtcttttg tggttcgaaa aagagtctgg
     2701 caaatggcat cccgatggca aacattctgt tccccacatc ggtgatcggt atgatggtgc
     2761 tgcccctgat gattttccat cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat
     2821 acaaacgcca gaccgaacag ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa
     2881 gcggacgttt caggggctgg accagttgcg tcagcccctc gattttaatc agcagcgtga
     2941 tttgcattag ctcaccgagc ttccctgccg ggaactcatc tttgcgggca aaccacagca
     3001 gatactcctc cggcaggtcg attaagcgac gccctttgta cttgccaaac ggcattatcg
     3061 tattggctat ttcaatcagc tgctcttttt ccatctcagc tacccagcaa acgcagcatt
     3121 tccgtttcgt cgatgacttc aatacccagt tcctgcgcct tcgccagttt agatcctgca
     3181 gcttcacccg ctatcaccag atcggttttc ttcgacacgc tgcccgcgac tttcgccccc
     3241 agttcgacca gtcgagcttt agcgtcatca cgcgacatct ggcttaagct gcccgtaagc
     3301 accacggttt taccagcaaa cggactgtca atctcttccg cattgataac gatcggctca
     3361 ggccagtgaa caccttccgc caacagctcg ctgatgacat tgcggttgct ttcttcggca
     3421 aagaagttgt gaacgtggga ggcaacgaca attccaacat caggcacctt ttgcagctct
     3481 tcaatcgacg cggcttccag cgcttccagc gtgccgaaat atgccgccag acctgctgcg
     3541 gtagcctcgc cgacttcacg gatgccaagt gcatagagga agcgggcaaa ggtggtttct
     3601 ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt tcggccccat acgctccagt
     3661 ccggtcagtt tgcctgcggt gagtttgaac agatccgccg gagtgtggac atattctttt
     3721 tcaaccagtt gatcgatgat tttgtcgccc attccgtcaa catccatcgc acggcgggaa
     3781 acaaagtgtt tcagagactc tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg
     3841 gcaaccgctt caccttccac acgctcgacg tcagaaccac ataccggaca atgcgtcggg
     3901 aatacaacct cacgggtatc ttccgggcgt tcagaaagca cgacgttaac cacctgcggg
     3961 atcacgtcgc cagcgcggcg aataaccact ttatcgccaa tgcgtaaacc aagacgttcg
     4021 atttcgtcag cattatgcag tgtagcgtta ctcaccaaca cgccagcaac atggacaggt
     4081 tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc
     4141 acaaaggtca tctgttcctg cgccgggaat ttaaacgcta ccgcccagcg cggggcacgc
     4201 gcgacaaagc caagctgctc ctgctgtgcc agtgagttga ccttaatcac cacgccgtcg
     4261 atatcaaagc ccagcgtcgg acggtctttt tctactttgt gataaaacgc cagcacttct
     4321 tccgccgatt cacaaagtgt tacccgatca ctgacaggta atccccatgc tttaaattgc
     4381 attaagcgtc ccaaatgggt atccggcaat tcaccgcctt ccagaacacc aacgccgtag
     4441 cagaagaaag tgagcggtcg cttcgctgta atacgcggat caagctgacg cagtgaacca
     4501 gctgccgcat tacgtgggtt agcaaacact ttcccgcccg tgcgtcgcgc atcttcgtta
     4561 atcttttcga accccgcctg cggcaggaac acttcaccac gcacttccag acgcgccggg
     4621 atattctcac cgtgtagctt cagcggaatg gcgcgaatag tacgcacatt agacgtgata
     4681 tcttccccgg tagtgccatc gccacgagtc gcggcactga ctaaaacgcc attttcatac
     4741 agaatactga cggcaagacc atccagcttc agctcacagc accaggtgac tttctcgttg
     4801 cttttcagac ggtcctgcac acgtttgttg aaagcaagaa agctttcttc atcaaaaacg
     4861 ttatccagcg acagcattgg cacttcatgg cggatctggc tgaaagccgc cagcggcgct
     4921 gcacctacac gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc
     4981 tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc
     5041 acatgataaa gatattcatg atggcgaagc gtcgttcgca gttctgtcag ttgttgttcg
     5101 attgattcca tatcgcacca tcaatgctaa aaacccccga caagcggggg ttcgaaaagg
     5161 agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg
     5221 gtactcgcgc aatttctgcg gagtcatcat acggcgctga tcgtcaagca ctacaccacc
     5281 cacttcatcg gcaatatgct gcgcagattg cagcatcagc ttgaagttct gcagctcgtc
     5341 accgtaagac ggtacctgca taaagatagt gacacccgga gtagtgaaat ccttcatttc
     5401 aggatcaaag gttcccggtt tcaccatatt cgccaggctg aataacgccg ggccgctgcc
     5461 atccgggcta agatgacgat ggtaaatgtt catatcacca aaaatgaagc ccgcttgttg
     5521 aatgctgtta agaagcagtt caccgtttaa ctcgctaccg tgatgcgccg cgacgttcat
     5581 gataatcacc gcttctttgc gcttcggttt atccataact ggagccggtt ccgcaacagg
     5641 ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg
     5701 tgctgaatgc actggctgcg gcgctgacgc gacctgtggc gaaactggct gctgcaacgg
     5761 ctgttcaggc tgcggctgat aggcaggctg ctgcacgggt tgctgcaccg gctgcgttgg
     5821 acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg
     5881 ctgcgcagac gcataaggcg gttggtactg gtgttgcggc gacggacgag caacctcatg
     5941 ctcctgggcg ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc
     6001 atcatcttcg acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa
     6061 tggccgatcg cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag
     6121 taaagcgatt atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat
     6181 tatattctct gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat
     6241 ttgccgatta cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa
     6301 tcgtgctttt tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta
     6361 aaatacgcag aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc
     6421 attcacatct gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc
     6481 gcaacctggg attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg
     6541 gggcgcattc tggtggctct ttacgcaact cgatgtctgg atcccgactc tcatgagtta
     6601 tgttccggac tggttacaat ggctgagcta tctgttgtgg cccctggctg tcatctctgt
     6661 gctgttagtg tttggctatt tcttctcaac gattgctaac tggattgctg ctccgttcaa
     6721 cggtttattg gctgaacaac tggaagcaag actgactggc gctacaccgc cagataccgg
     6781 aattttcggt atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgc
     6841 ctggtatctg ccgcgcgcaa ttgtattgct aattctttac ttcatccctg gcattgggca
     6901 aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg
     6961 tgattacccc ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac
     7021 acgcaaaatc accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct
     7081 gcttaatctg ttcattatgc ccgttgccgt ttgtggcgcg acggcgatgt gggttgattg
     7141 ctatcgcgat aaacacgcca tgtggcggta acaatctacc ggttattttg taaaccgttt
     7201 gtgtgaaaca ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct
     7261 tctgcatata gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct
     7321 gttgtatccc aatttcatac agttaaggac aggccatgag taagattttt gaagataact
     7381 cgctgactat cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc
     7441 tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca
     7501 tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccaa
     7561 ccagcggtaa taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactta
     7621 ccctgaccat gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg
     7681 caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag
     7741 aaattgtcgc cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa
     7801 accctgaaat tcatgaaaaa accaccggtc cggagatctg ggaagatacc gacggtcagg
     7861 ttgatgtatt tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca
     7921 ttaaaggcac caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc
     7981 cagttatcgc ccaggcgctg gcaggtgaag agattaaacc tggtccgcat aaaattcagg
     8041 gtattggcgc tggttttatc ccggctaacc ttgatctcaa gctggtcgac aaagtcattg
     8101 gcatcaccaa tgaagaagcc atttctaccg cacgtcgtct gatggaagaa gaaggcattc
     8161 ttgctggtat ctcttctgga gcagctgttg ctgcggcgtt gaaactacaa gaagatgaaa
     8221 gctttaccaa taagaatatt gtggttattc tgccgtcatc gggtgagcgt tatttaagca
     8281 ctgcattgtt tgccgatctc ttcactgaga aagaattgca acagtaatgc cagcttgtaa
     8341 aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc
     8401 cctcctggca ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg
     8461 cgcgaaatta atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat
     8521 tcttccttta gcggcataat gtttaatgac gtacgaaacg tcagcggtca acacccgcca
     8581 gcaatggact gtattacgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata
     8641 caggctaaag tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg
     8701 gggaaataca atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg
     8761 ccctgctgcc cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc
     8821 caacggcaaa agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca
     8881 aggtaccgtt gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct
     8941 ggttaaactg atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag
     9001 taaggtaggg ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct
     9061 ctgcttctga aagaagacga aattatcatt gaccggaaaa aaatttctgc cgaccaggtt
     9121 gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg
     9181 atcaaaacga aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat
     9241 attatgctgc tcgaagatga ggagctggag caggaaatca tagccctgat taaagataag
     9301 cacatgacag ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa
     9361 gagctggatg atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc
     9421 ctgctgcgca acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc
     9481 attctggttg ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg
     9541 ctgggtttca tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct
     9601 ctggaactgc ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac
     9661 tatctgattc tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt
     9721 gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg
     9781 aaagatctgc cagctattac gctggacggt caccaggtag aagtgtgcgc taacattggt
     9841 acggttcgtg acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt
     9901 actgagttcc tgttcatgga ccgcgacgcg ttgcccactg aagaagaaca gtttgctgct
     9961 tacaaagcag tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc
    10021 ggcggcgaca aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc
    10081 tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgtgct
    10141 atcctgcgtg cctctgcttt cggtaaattg cgcatcatgt tcccgatgat catctctgtt
    10201 gaagaagtgc gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa
    10261 ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca
    10321 acaattgcac gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta
    10381 acgcagtaca ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccagcca
    10441 atgtcaccgt ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc
    10501 aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg
    10561 gggatgggtc tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt
    10621 atccgtaaca cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg
    10681 acaacggacg agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa
    10741 tccacgagat gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact
    10801 gaaatctctg gtttccgacg acaagaagga taccggaact attgagatca ttgctccgct
    10861 ctctggcgag atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt
    10921 tggtgatggc attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggcac
    10981 cattggtaaa atctttgaaa ccaaccacgc gttctctatc gaatctgata gcggcgttga
    11041 gctgttcgtc cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat
    11101 tgctgaagaa ggtcagcgcg tgaaagttgg tgatactgtc attgaatttg atctgccgct
    11161 gctggaagag aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat
    11221 caaagaactg atcaaactgt ccggtagcgt aaccgtaggt gaaaccccgg ttatccgcat
    11281 caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt ttttatgctt
    11341 cagccagagg cggcaaaatc aattcatcgc tctcatgctg ctgggtatag cgcatcactt
    11401 ccagtacgcg caaccccgcg cggtgcaccg catcggttaa cgccttccct ttcagcaaac
    11461 cactgataag ctgagcacaa aacaggtcgc cagtcccttt caggtcagtt ttaacccggg
    11521 aatgggaaat gacattcacg ctgtcggcac tgaccaccac aacctgcatc tcctgatttt
    11581 cttcattgcc ggaggcgctg gtaatcacca cccactttaa tgtgtctgaa agcagacttt
    11641 ttgcggcagc aatggcactg tcgagatcgc ggcaatcttt accggtcagg atttccagct
    11701 caaagatatt gggggtaatt ccctgtgcca gcggcagtaa atattgtcga tacgcctcgg
    11761 gaaggtcagg tttgacataa attccgctat caatatcgcc aatcaccgga tcgaccatga
    11821 tcaataggtc aggatggtct ttgcgtagcg cagtcagcca ctcggcaagg attttgattt
    11881 gcgatgccgt tcccatatag cccgtggtta cagcacgaag ttggcgcagc gcatcacgct
    11941 cctgaagcgc acgcaaataa ccgctaaacc attcatccgg aatcgcacca ccgtagaaag
    12001 tgtcataatg cggcgtattg ctcagcaata ccgtcggcac ggcaaagaca ttcaggccgt
    12061 tctgctttat agcaggcacc gcaatgctgt tgcctacgct gccgtacact acctgcgact
    12121 gcacggcgac gatatccgcc tgcagtgccc tgctcttatc gttaaacaac aacaaactac
    12181 tcatttaatt ttttctcctt gccgatgatc ctcatcgtat tccagccgaa actttacctg
    12241 attctggcag tcaaatcggc tattacaaaa caaggataag gtaattcagt gaagaaaatc
    12301 atttgtctgg tcgttacgct gctaatgaca ctccccgctt atgcgaagtt aacggcccat
    12361 gaagaagccc gtatcaacgc catgctggag ggattagcac agaaaaagga tttgatattt
    12421 gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt ctcatctgcg cctgaagctg
    12481 ggcaataccc gtaatcgcat tgatacagcc gagcagttta ttgacaaggt ggcctcgtcg
    12541 tcatcgatta ccgggaagcc gtatatcgtg aagatgcccg gtaagagcga tgagaacgca
    12601 cagccttttt tacatgcgtt aattgcgcag acggataaaa cggtgcctgc gcaataatct
    12661 taaatccccg ccccctggct aaaatgctct tccccaaaca ccccggtaga aaggtagcga
    12721 tcgccacgat cgcagatgat cgccaccacc accgcgccag ggttagcttt tgccacccgc
    12781 agtgctccgg caaccgcgcc gccggagctt acgccgcaga aaatcccttc ccgcactgcc
    12841 agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccaga
    12901 gaagcattga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc gggaatactg
    12961 ctgccttctt ccggctgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag
    13021 cgtgacacgc cggtgatagt gccggttgtt cccatgctgg agacaaaatg ggtaatacgc
    13081 ccgccagttt gctgccagat ttccggcccg gtagtggtgt aatgggcgta agggttatcg
    13141 ggattattga actgatcgag caactttcct tcgccgcgat tcgccatctc cagcgccaga
    13201 tcgcgtgcgc cttccatgcc ctgttctttg gtgaccagaa tcagttctgc accgtaggca
    13261 cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag
    13321 cctttaagcg ccgcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatc
    13381 agcacatcac ccggtttaat ctcgccgcgc ttttccgcct cgacaatcat cgaaagcgcc
    13441 gcacggtctt ttaccgatcc cgccgggtta ttgccttcca gttttaacca cacttcactg
    13501 ccgttatccg gcgccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct
    13561 aatgtactca cgatctctgt ccatacgtgg tgttgcctga tgcgacgctt acgcgtctta
    13621 tcagacctac aggttacaaa accttgccat taaaaaaccc ggatcgcggg aagcgcctcc
    13681 gggcgtttaa cattcactca acctatcagg cgctttgtgc gagagcaagt tcctcatcgc
    13741 gggtttcaat acgctcatcg ccgttataca gccgcgcatg ttgcagacca acgtataaac
    13801 gatcgccacg ctgcggggca tcgtcgccat gcatcacgac cgtcagcggt tcgttgtacc
    13861 accccagcgg ctgcaccact aattgggtgt agtgaccttt cgggctggct tccagtacct
    13921 gtacgggtag cggcgaatcg aggctggtac ggcggctgat atccacttcc cacgggcgca
    13981 ggaagagatc caccggcccc tgatacgcgg gggtgtatcc cagcggccag cgatgtgcgc
    14041 caacgtggaa ctgcccgccg cgaatggttc cctgcaggcg gtttacctcg cccataaact
    14101 ccagcacaaa acgagtcgcc ggttcacgcc atacctgatc cggtgcgtca gcttgttcaa
    14161 tattgccctg actcatcacc actacacgat cagctacttc ggtcgcctct tcctggtcgt
    14221 gggtcacgaa cacgctggtg aatttcagtt cttcatgcag ttgacgcagc cagcgacgca
    14281 gctctttacg cacctgcgca tccagcgcgc caaacggttc atcaagcagc aaaatttgcg
    14341 gttccacagc cagcgcgcgt gcaagcgcca cacgctgttt ctgaccgccg gaaagctgcg
    14401 ccggataacg atccgccaga tgagcaagct gcaccatctc cagcaatttt gtcactttcg
    14461 ctttgatcgc cgccgcattc gggcgttcac gccgtggcaa caccgtcagg ccaaaagcga
    14521 tattgtcaaa caccgtcata tggcgaaaca gcgcgtaatg ctggaacacg aaaccgactt
    14581 tacggtcccg cgcgtgcaga cggctcacgt cggtaccgtg aaagcgaata tgcccactgg
    14641 tttgatgctc cagcccggcg ataatgcgca gcagcgtagt ttttccggaa ccggacggcc
    14701 caagcaacgc gaccatctga ccagaaggaa tatccagtga gatatcgttc agcacctggg
    14761 tgcgaccaaa agacttctta atattggcaa tctcaatgct catgatgttc ctcctgctgc
    14821 gcgcgttttt cctgattttc caggcgccac tgcaacatac tctttaaaaa cagggtgata
    14881 atcgccatca gcgtcaacaa cgcggcggcg gtaaaggagc cgacggtgtt gtagtcctgc
    14941 tccagcaatt caatctgtaa cggcagcgac agggtttcgc cacgaatcga gccggaaacc
    15001 accgataccg cgccaaactc gccaatcgcg cgggcgttgg tcagcaccac gccgtaaagc
    15061 agcgcccagc ggatgttcgg cagcgtgacg cgacggaaca tctgccagcc ggacgcgcca
    15121 agtaaaatcg ccgcttcgtc ttcctggctg ccctgactta acattaccgg taccagttcg
    15181 cgcaccacaa acggacacgt cacgaagatg gtgaccagca ccatacccgg ccaggagaac
    15241 ataatttgca gattatgttc atccagccag ccgcccagcg ggccgttaga gccgtagaac
    15301 agcagataca ccagaccagc aaccaccggc gacacggcaa acggaatgtc cagcagcgtc
    15361 agcaatagct ggcgtccggg gaagttaaag cgcgtcacca gccaggccag cagaatgccg
    15421 aacaccaggt ttaccggtac ggcaatcagc gcgatcatca ccgtcagcca gatggcgtgc
    15481 agcatgtccg gatcggccag attttgtaac accggcatca gtcctttgct gaacgcctgt
    15541 acgaagatat aaatcatcgg caccagcagg atgaacgccg ataccagcat cccgatgcca
    15601 atcagaaacc atttgcccca gttaatcggg cgcgcgtcat aacgcttcaa ttgtgtcact
    15661 tccgccatta atgacctacc acacgccgac caaagcgact ttgcagagtg ttaattgaga
    15721 acagcaacag cagcgatgcc gcgaggatca ccgaggcaat cgcgctcgcc gccgggtaat
    15781 caaactcctg taagcgaaca aagatcatca gcgacgtcac ttctgttttc caggcgatat
    15841 tcccggcgat aaaaatcacc gcgccaaact caccgaggct gcgggtaaac gacagcgcca
    15901 cacccgccac cagtgccgga gatagttccg gcagcaccac tttgcgaaaa ctctgccagc
    15961 gtgttgcgcc gagcgtttct gccgcctctt catattccgg gcctaactct tccagcaccg
    16021 gctgcacggt gcgcaccaca aacggaatgc tggtaaaggc catcgccacc gcaatcccca
    16081 gccaggtata ggtgactttg atatcaaact tcgccagcca ttcaccgtaa aaaccgttca
    16141 cggaaaagag cgaggccagc gttaaaccgg cgactgccgt tggcagcgca aagggtaaat
    16201 ccatcagcgc atcaagcagc gtgcgacccg gaaagcgata gcgggttaag atccacgcca
    16261 tcagcagacc gaaaacgccg ttaaaaatcg atgccacaaa cgccgacagc agcgtcactt
    16321 tataggctgc gaccacctgc gggttggtga tcacctccca gtactgcgcc cagctcatct
    16381 gggccagttg catcaccagc gcggagagcg gcagcagcaa aatcaggcac acaaacagca
    16441 gactggtgcc gaggcttaag gtaaagcccg gcagcacgcg tcttgaagaa actgcaaaca
    16501 ttacttacgc cccgccgcta acagcttgtc taactcgccg ccgctggtga agtgggtttt
    16561 catcacttcc ggccaggagc caaatttatc ttccacgcgg aacagctcgg tctgcgggaa
    16621 tttatctttc agtttgccca tgacttccgg gttatttacg cggtagtaat agtcggtgat
    16681 gatggtttgc gcctgcgggc tgtagagcca gttcaggtag gcttttgccg ctttttccgt
    16741 gccgttggcc tgtacgtttt tatccaccca cgccaccggg aattccgcca gaatgttggt
    16801 tttcggaatc accacttcaa agccctgcgc ttcatactgt ttacggatgt tgttcacttc
    16861 cgactcgaag ctgatcagca catcgcccag gccgcgctcg gcaaaagtgg tggtcgcgcc
    16921 acgaccgcca gtatcgaaca cttcaacgtt tttcaggaac tgggtcataa actgttcggt
    16981 tttggcttta tcgccaccgt cggctttatc cgctgcaccc cacgcggcta aataggtgta
    17041 acgcgcgtta ccggaggttt tcgggttcgg aaaaatcagc ttcacgtcgg agcgcaccag
    17101 atcgttccag tcgtggatat tcttcgggtt acccttacgc accaggaagc ccatggtgga
    17161 gtagaacggc gagctgttgt ttggcagacg cgactgccag tcagccggaa taagcttgcc
    17221 tttgtcgtgc aggatctgca cgtctgtcac ctggttataa gtgacaacgt cggcttttaa
    17281 gccctgcaaa atcgccagcg cctgttttga tgacccggca tgagattgtt ttatcgtcag
    17341 tttgtcgccg ccgttatctt tggcccattg ctgttcaaac ggcgggttca gggcggcaaa
    17401 cagctcgcgc gagacgtcat aagaactgtt cagcagttcc gttgcctgta catggcccgc
    17461 cagcagcaga gaagcgacca gcacgagtga gttctttttc agtaagttaa cggccattgc
    17521 gcacccttat aaatttaatg actttctaat agccatcata tttataacgg acgtgaaagg
    17581 agtaacggtt ttatataccg tttggtgatt tggaagttga aaagggaata agacgaaatc
    17641 ctccccacaa atgcagggag gaaacagagg tgaatcagat gccgacgcta accgtctccg
    17701 gcagtgtgct gccgccatca atcacattct gtgtacctgt taaatagctg gattcatccg
    17761 atgcgaggaa ggccgccagt tcgccgactt ccagcggatc ggcgaggcga cgcatcggga
    17821 ttgctttcgc catttcagtc agcaccgatt ctggatcttc cgggttcgac tggcgggcaa
    17881 tgctttccgc cattggcgtg cgaacgtatc cagggcagat ggcgttaacg cgaataccag
    17941 actgcgcgta ctccaccgcc agcgatttag tcaggccaac aatcgccgct ttcgttaagg
    18001 cgtacgccgt ttcgcccggg tctgccacca tatcgccagt gactgaagac atcatcacaa
    18061 tgcggccatc tttgcgggca atcatctccg gcagcaccgc cttcgtgacg ttccatacgc
    18121 cttttatatt gatatcaata tggaaatcgc gatcttcatc gctcatatcg aggaagctgc
    18181 ccagacgaca aacgcctgcg ttattcacca gaatatcaat gcgtccttct ttttccttag
    18241 cgcgcttaat agctgcggct accgacgccg ggtcacgcac atcggcgaca accgccgtac
    18301 agcgatgacc acgaccacac agttcgtccg ccagcttttc gatctcaggg gagatatcca
    18361 gcaagattag gtttgcgcca tgacgtgcaa aagttctggc aattccttcg ccaattccct
    18421 gcaatgcgcc cgtaatcagt gctgtcttgc ccgtgagttt acccatttta atgctctcct
    18481 tgttaataca gagcgttaca ctccattccc tctgaaaata caggataaac aaaatattgc
    18541 tgcggatcag ctcactaatt caggaaaatc tcaatttagt cgctgcttta tgcagcacct
    18601 caagcgttac gtcagcgcgc aatcattttc agtgacatca catgagtaat attcggactt
    18661 aatgattcct ttgtgtctat caaatcggga tatatgatcc catttatctg caattatttg
    18721 atcgcaaata ttaaggtcac caccgatgca acttgaaaag atgattaccg aaggctcgaa
    18781 cgccgcctcg gctgaaattg accgcgtatc aacgctggaa atgtgccgga ttatcaacga
    18841 tgaagataaa accgtaccac tcgccgttga gcgcgtactg ccggatatcg ccgcggcgat
    18901 cgatgttatc cacacccagg taagcggcgg cggccgtctg atttatctcg gtgcgggaac
    18961 atccggtcgt ctggggattc tggatgccag cgaatgtccg ccgacctacg gcgtgaaacc
    19021 gggcctggta gttggtttga ttgctggcgg cgaatatgcc attcagcacg cggtggaagg
    19081 cgcagaagat agccgggaag gtggtgttaa tgatctgaaa aatatcggct taaccgcgca
    19141 ggatgtggtg gttggtattg cggccagcgg acgcacgccg tatgtgattg ccgggctgga
    19201 atacgcgcgc caactcggct gccgtacagt gggcatttcc tgtaatccgg ggagtgccgt
    19261 ttcatccacc gcagagtttg ctattacgcc ggtagttggt gccgaagttg ttaccggttc
    19321 ttcgcgaatg aaagcaggca cggcgcagaa actggtgctc aacatgctct ctaccgggct
    19381 gatgattaaa tccggcaaag tgttcggcaa cctgatggtc gatgtggtcg ccaccaacga
    19441 aaaactgcat gtgcgccagg tcaatatcgt caaaaacgct accggatgta atgccgaaca
    19501 agcggaagcg gcgttaattg cttgcgagcg caactgtaaa acggccatag tgatggtact
    19561 gaaaaatctc gatgccgctg aagctaaaaa atgcctggat caacacggcg gttttattcg
    19621 caaggctctg gaaaaggaat aacccatggc taaagagatc agcagtgaac ttctaaacac
    19681 cattcttacc cgggtcggcg gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg
    19741 cctgcgtctg ggtgtacatg acagttcact ggttgatccc aatatcaaaa cgctggaagg
    19801 cgtgaagggg gtcattttga ccagcgatca ggtgcaggtg gtttttgggc caggcaaagc
    19861 gcatcgtgcc gcaaaagcca tgagcgagct gctgggcgag gcaccggtac aggatgccgc
    19921 agaaatcgcc gcccagaata aacgccagtt aaaagccaga caaacttccg gcgtgcaaca
    19981 atttctcgcc aaattcgcca ccatcttcac gccgctgatc cccggtttta ttgccgccgg
    20041 tctgctgctg gggatagcga cgttaattgc cacggtgatg cacgttccgg cagatgctca
    20101 gggcacgcta cccgatgcgc tgaattttat gaaggtgttc agcaaaggcc tgttcacctt
    20161 cctggtaatt ctggtgggct ataacgcggc ccaggcattt ggtggcacgg gcgtaaatgg
    20221 cgcaattatc gctgcgctct ttttgctcgg ttataacccc gctgcaacca cgggttacta
    20281 cgccggattt cacgatttct ttggtctgcc tatcgatccg cgcggcaata ttatcggcgt
    20341 gttgattgcc gcatgggcct gcgcccgcat cgaatgcatg gtgcgccgct ttatgccgga
    20401 cgacctcgac atgctgttga cctcattaat caccctgctg atcaccgcta cgcttgcgta
    20461 cctgatcatt atgccgctgg gcggctggct gtttgaaggt atgtcatggc tgtttatgca
    20521 cctgaacagt aatccgctcg gttgtgcggt tttagccggg ctgttcctga tcgccgtggt
    20581 atttggcgtg catcagggct ttattcctgt ttatctggcg ttaatggaca gccagggatt
    20641 caacagctta tttcccatcc tttcaatggc aggcgcaggc caggtgggcg cggcgctggc
    20701 gctctactgg cgggcgcaac cgcacagtgc attacgcagt caggtccgcg gggcgattat
    20761 tcccggtctg cttggcgttg gcgaaccgct gatttacggt gtcaccctgc cccgcatgaa
    20821 accgtttgtt accgcctgtt taggcggcgc ggcgggcggt ttgtttatcg gcctgatagc
    20881 ctggtggggt ctgccgatgg gcttaaacag cgccttcggg ccgtctggtc tggtggcgct
    20941 gccgctgatg acttccgcac aaggcatcct tccggcaatg gcggtttacg ctggcggtat
    21001 tctggtggca tgggttagtg ggtttatttt caccacactc tttggctgcc gtaacgtcaa
    21061 tctggactga ttatgaaacg gacaatgctc tatctttctc tgctggctat tagctgtagc
    21121 gtcagcgccg caaaataccc tgttttggca gaaagtagtt gatcaagtgt atgcggcgtc
    21181 aaagcagtaa taaaaaggcc agtcagttgt gttactggct ggcctttaat caatgaatca
    21241 gaaacgctta cagcgccatc agcttgtcca gcgacggtgc gaagtaatag ccgccagtga
    21301 ccggtttggt gaaacgcaac atcgcatcac gcttaccatc ggtatcgcca aacatgctca
    21361 gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag
    21421 tgccactggc ggtgccgtac ggcaggctct gacgaacaat cttcagccct ttgccatctt
    21481 ctttcagatc aacgcgggtc aggtgagagg tttccggacg ttcgtcgccg tcaatctctt
    21541 cgttggcctc tttggtgcgc ccaatcatca tctcttgatc gtgaacgctc atccgattga
    21601 gctgcttcag gttgtgctcc caacgctgca caaacacata gctgccgccc gcatccacgc
    21661 cgtctttgat aacagccact tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt
    21721 caacaaagcc gctcaggtca cgctcttcaa cccaacggaa gccatggatc tcttctttca
    21781 cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc gacagagaag ttcacgtcgt
    21841 gacgcagaga aagaatgtgg atcaacacat cgaactgggt cgtcggtgca agacctttac
    21901 cgtagcccgg aaaatctttc agctcttcgg ccccaacgcc gccgctcaga gcgcgccagg
    21961 tattgttacc aaaggcaacc accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag
    22021 ttgccagttt gtcggcaaat gttttactgg ccgcacgcag ggcgtcaact tcccctttca
    22081 cgttggcttc gatccaaatc gccgcgcggc aatgttctgg caaaatgcca ctctgaacct
    22141 gagacattgt tcctcctgaa aataataatg ccacgcaatc cgtggcattg ttgggcgcta
    22201 ttttacgtgt tttttcgcgg cggcggtttg ttcagacgca aattaacgcc gccagataat
    22261 tttactgact ttccagtttt tcagggtatc gtccgaaggc attaaccctt caggaccact
    22321 ccattccccg ctgaactggt agctaatatg ttgactgcct tcggctttac attcgacggc
    22381 acgattatca tcactgtcgg ccttttggca attgccaaat gctttgctgt aaaggtcact
    22441 aaacggtgta ccgattttaa caccggtgtc agcaggaata tcgctatcca gcacatcaat
    22501 gcggctgatc gtgccttgat cgccgttaat caccatcgcc acgttgttgc ctttcatcac
    22561 ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt ccgctgcgca ggcgataatc
    22621 gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt gtggacgccg ttaattcacc
    22681 cacgccctgc tcgctcactt tggtggatga tccaaaccag ttccacgggt tagcggccga
    22741 ccagttaact gacgacatcg tggaacagcc ggtcagcatc aacggcatag cgcataacat
    22801 taaacgcagc gatttcatgt cacttccttt ggttattcaa taacgttgct tggagtgcaa
    22861 attcaccaaa aagtgccgtt attctttttc ttcattaaaa caagcgcgta aacgtcgatt
    22921 ggtcagcagc cagattaacg ccacgatatc cgccactacc agcgccagac cgataccgct
    22981 aacggattca ccgttcagcc acagccacgg ttgccaacaa agtaaaacca cttgcgccaa
    23041 cagcaacaga aaatagagca catgccagat acggggaaat gaagcccgcc gaccactcag
    23101 caaaaatgcc agcaccgccg gaataccggg aatcaacccc agccagaaat tatcgtgatc
    23161 gggataaaac agatttagca gcgcagtacc ctgctcgcgc gacgctccgg caatgacaaa
    23221 cagcacccag gttcgcgcct gaagcaatag cacaagccag aagagcaagg gtaaacgcag
    23281 gcgaccgtgc gcatcataat ggacaggatg aaactcagta ctcttcatct tcaatcaaac
    23341 gcttacccag actcagcacg tcggcgtgtt catatcccag gcgttcatac attccgagca
    23401 ccatgtcgtt atcttccggc acgttgatct gaattttcgg acagccgcga gcaatcagct
    23461 ttttctccag ccgattaagc aacgcattag caatcccacg cccacgaaac tcgggatgca
    23521 cgccaagata ataagcagac ccgcgatgcc cgtcataacc gcccatcacc gttccgacca
    23581 cttcaccgtt cacctcagcg accagaaaca aactgacgtc atggttcatc ttacgctcga
    23641 tgtccatttc cggatcgttc cacggactca gcaagtcgca acgctcccaa agggtgatga
    23701 cctcttcgaa atcttcctgg cgaaatacgc gtatctccat ggtattcgtt acctttttgc
    23761 gggttaaaag gctgattatg gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg
    23821 ttgaaaaagt ggcataatgg ggagttgtca actattgaaa tgaaaagtaa aacaattctc
    23881 aacagcaaac cgtcgtaacg gattacgcga tacgatataa catctggaac tttattatta
    23941 caactcaggc cgtatgagca cttttaaacc actaaaaaca ctcacttcgc gccgccaggt
    24001 gctgaaagcc ggtttggctg ccctgacgtt gtcaggaatg tcgcaagcca tcgccaaaga
    24061 agaaccgtta aaaaccagta acggacacag caagccgaaa gcaaaaaaaa gcggcggcag
    24121 acgtgtcgtt gttctcgatc caggccacgg cgggattgat accggagcga tcggacgcaa
    24181 cggttcgaaa gaaaaacatg tggtgctggc aattgccaag aatgtccgtt ccatattgcg
    24241 taatcatggc attgatgcgc gtttaacgcg ttctggcgat acgtttatcc cactgtacga
    24301 tcgcgttgaa atcgcccata aacatggcgc agatctgttt atgtcaattc atgccgacgg
    24361 ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc ctctctaacc gcggggcaag
    24421 tagcgcaatg gcaaaatacc tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa
    24481 aaaagcgact gacaaggatc acctattgca gcaagtgctg tttgatctgg tgcaaaccga
    24541 tactattaaa aacagcctga cgctcggctc gcatattctg aaaaagatta agccggtgca
    24601 taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt
    24661 tccgtcggtg ctggtggaaa cctcgtttat caccaacccg gaagaagagc ggctgttagg
    24721 cacggcggcg tttcgccaga aaatcgccac agcgattgcc gaaggcgtga tcagttattt
    24781 ccactggttt gacaaccaga aagcacactc gagaaagcgg taagtgatga aacccgacgc
    24841 acacctggtt aaacagttct tgctcaacct tcaggataca atttgtcaac aactgaccgc
    24901 cgtcgatggc gcagagtttg tcgaagatag ctggcaacgt gaagctggcg gcggtgggcg
    24961 tagtcgggtg ttgcgtaatg gtggtgtttt cgaacaagcg ggcgtcaact tttcacatgt
    25021 tcacggtgag gcgatgcctg cttccgccac cgctcatcgt ccggaacttg ccgggcgcag
    25081 tttcgaggcg atgggcgttt cactggtagt gcatccgcat aatccgtatg ttcccaccag
    25141 ccacgcgaat gtgcgctttt ttattgccga aaaaccgggt gcagagcccg tctggtggtt
    25201 tggcggcggc ttcgatttaa cccctttcta tggttttgaa gaagacgcca ttcactggca
    25261 ccgcaccgcc cgtgacctgt gcctgccatt tggcgaagac gtttatccgc gttacaaaaa
    25321 atggtgcgac gaatacttct acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct
    25381 gttctttgat gatctgaaca cgccagattt cgaccactgt tttgccttta tgcaggcggt
    25441 aggcaaaggc tacaccgacg cttatttacc aattgtagag cgacggaaag cgatggccta
    25501 cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct
    25561 ggtctgggat cgcggcacgc tgtttggcct gcaaactggc gggcgcaccg agtcgatcct
    25621 gatgtcaatg ccgccactgg tacgctggga gtatgattat cagccaaaag acggcagccc
    25681 ggaagcggct ttaagtgagt ttattaaggt cagagattgg gtgtaactcc ctcaccccca
    25741 ctctcgcatc cgctgatgca gcgtcagtga cggcttctcg gcaaacagtt gctgataatc
    25801 cgtggcaaat tgcccaagat gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt
    25861 gctttgtgac cacggactta tgagttcgcg gcgcacggcg ttcaggcgaa tacgtttcag
    25921 ccacgcgttc ggaccaatgc ccaaaatggc gtgaaatgcg ttttgtagcg tgcggcggct
    25981 gacatgcagt tgattacaca agtccagcac cgtcaccggt tcggacatgt tttccagcac
    26041 atattcacgg gcgcgggaaa gcaatcggcg gtaactctga tggctgatgc tttccgccgt
    26101 catcatcggc tgcgcttctt ccagcatcgc ccccatcgcc atgagcaaat tatcccccag
    26161 cacttttcgc actgcaggct gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc
    26221 ctgttggaca aagccccaca gagcggcttt atgctgctct ttcacttcca gcgccgactg
    26281 gctacgcagc atatgtaata cccgatccgg attatgcaaa aagttagcct gacgagtgat
    26341 gacatcttca gaaagcacta cgccaaggat cgtgtaatcg tccggcgtac ttaattcaaa
    26401 ctcggttcca cccgggcgcg tcgcaatttc tgcgcttccc agacattgcg aaccgataaa
    26461 tcccagctca ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca
    26521 cgactgacgc agcgccagac cagtgtattc acgaaaaacc tgaatatcat cgagtagaat
    26581 ttccgtaaat tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgccaggc
    26641 ggtaattgtc aaagcatgtt catagacgtc cgttgtctgt cgttgatgaa cattatccac
    26701 ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa
    26761 attggctgta cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc
    26821 tccgacaggt taatggggct tgagacggta acgactactg cgtttacgta acgtcccggc
    26881 agaaaagagc tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcagc
    26941 cacttctcct gccgtcattc cctgacgtac tgatgttaac agcgccaaca actcgtcagc
    27001 agattccgcg acgataactg gcgcgtcggg ttcctttacg ggttgcttag gctggcggtt
    27061 aaagccagta accagccact gggtgtcatc gtctggccga ccgatctcct tgcggctgat
    27121 gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc
    27181 cagatcgcct tccaccacca gcgttaggcg accagggtca agcacttcgt gactgagcag
    27241 acgaacgtta gctgctttga gcatggcatc agctgcatct atcgcggcga ccatgccgtc
    27301 cacttccagc aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg
    27361 attacgcgcg atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc
    27421 ctggctaccg gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacataggt
    27481 caccagctgc acgtcggcag atttcaacgc tgcatcaatg ccgtaggtcg cttccagcgg
    27541 cggtgccacc agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgagagata
    27601 agaaccggta cgcgaaacta catgtgccag gaaggctgtg ttttctgcgt cattagccca
    27661 ctggaaagca gcgccatttt caatattcgc aaccatcgca tccagaccag cacgcacttc
    27721 cgccgggttt ggtccaccaa gcataatcag cacctcaccg gcagtcggtg acgggccgtg
    27781 tgcagcgccc gcatacagcg agcgaccata taccacttcg accatcgcct gcttggtcgc
    27841 ttcgtcagcc gcaatgtagg tgacgtcatc tgaatcagca gaaatcagcc cgaggctacg
    27901 aatatgcggc ggcaatttca gttcacgtgc aaattcggcg ttaacggagg caatcacccg
    27961 catggcggtt accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt
    28021 catgttgatg ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc
    28081 tgcggcttca accggcggcg tgccgccctg gtgaatgtta gagatacaag tgcggtcagc
    28141 ctcaacggtg gtcgccatac gcggcgagta aacggcgtag caggagaggc tttctgactg
    28201 ccccaacccc ggacgttcgc ccaccagcag gatcactact ttcgcgccga ggatctcacc
    28261 gatctgatct tcaatcttca cgcgaccata acgtacaaag aatggcgtac cgactttcag
    28321 cccggcttgc ttcaggcccg ccatcagcgg cggcaggatc tcttcgtagt tcacggtgat
    28381 cgcatcagtt gacaggccat cagaaataac gacctgcacg tccggattag caacacactg
    28441 tgctttcagc gcttcaacag cttctgcaca caggcggcgg cccatatccg ggcgagtcag
    28501 gtagagattt ttgtcgctga tttcggagcg gacttccagc aagccctgcg ctttcaccca
    28561 ctcttccggt acttctttca gaacggtatc tttcgaacga gagtgatcgg ccaggaaacg
    28621 cagcaacgcc tgggtgcgcg gacgcggacc ggcacgaccg gtacaaacgc gggccacggt
    28681 gctgcgacgc agttctgtta atacgtctgc gtgatgcggg ttttcaacac caatccacgc
    28741 ttttgcttcc gcagaaccta aatccagcgc gcagctttcc gaggtcaccg gtgccgcaca
    28801 ggtggtggtg gcgcactttg cttctgacgg cgcgggggcc gcttgtccca ttgacgccat
    28861 cacgctgcgt acaatttctt caatctgttt ttgatccatg atgtgttatc tccgcgtcat
    28921 cagaagaaca gtgacggatc gcccgcccgt ttggtcaggc gaccgtttgc cataatgccc
    28981 atgctttcca gccagcgttc aaactccggt gacgggcgca gattcagtaa ctgacgcaca
    29041 gtggcagtgt cgtggaatgc ggtggtctga tagttgagca tgatgtcatc gcccagcggc
    29101 atccccatga tgtagttgca gcctgcggtg gcgagcagga tcatcaggtt ttcgttgagg
    29161 ttctggtcag cgtcagcgtg gttggtgtag cagcagtcac agcccataga gatgccgctc
    29221 agtttgccca taaagtgatc ttctaagcct gcgcggataa tctggcggtc gttgtagaga
    29281 tactccggcc caataaagcc gaccacagtg ttgacgataa acggatcgta atgacgcgcc
    29341 agcccatagt tacgcgcttc catcgtcacc tggtctgcgc cgaagttcgc gccagcagac
    29401 agcgccgaac cttgtccggt ttcgaagtag aggcagtttt ccccggcgat acggttgaac
    29461 tccgcgccca ctgcgcgtgc ttcatcgagc atcgccagct ctacgccaaa ctctttcagc
    29521 cctttttcgc tgccgcagat actctggaaa atcagcccgc ctggtgcgcc gcgacgaatc
    29581 gcttcgattt gggtagtgac gtgcgccagt atgcagccct gagttgggat gttgaatttg
    29641 tcgatcacgc catagatggt atccaacacg cggcttaagt tttccacgtc gtcagtcacc
    29701 gggttaacgc cgatcaccgc atcgcccacc ccgaaggaaa gcccttcgta gatttgcgcg
    29761 gcgatacttt gcacgtcgtc acgggtatcg ttcggctgca agcgggcgct gaaggtgccc
    29821 ggaataccga tggtggtatt ggcctttttg atcaccggca ttttcttcgc gccgtagatc
    29881 aggtccgcgt tggagcaaat cttcgctacc gccgccacca cttccgaggt cagccctttg
    29941 cgggtaaagg caatgtcatc cacgctggtt tcatcgctca gcacatactc acgcagttcg
    30001 ctgatgctcc agtttttaat ctggttgtag gccgtttcgt taacgtcgtc ctgaatcagc
    30061 cgcgtcacgc agtcatcttc ataggcaatc accggattat tgcggatgtc cgctacagtc
    30121 atttccgaca acacctgctt tgccgccacg cgctcctgtg agcttgccgc agcaacgccc
    30181 gccagcacat cccccgaacg cagttcgttg gctttagcca gcacctcttt tacatcctta
    30241 aactgatata cattgccgaa caatgtggtc tttagtttca taagtcgttc cctcaggaag
    30301 gaaatgcgag tgatttcacc gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa
    30361 tgtcgatata gtcccccgcg cggacaatca cttcgtcaat gactgccaac gggagttgtt
    30421 gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa gtcctgcccg gccaccacca
    30481 gcaggggatg cggattcgga aaacgcgcga cgaaatcgac cagcgcgttg atgaccgtca
    30541 gtaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc cagcacgtac gcgtcagttt
    30601 tggggcaaag atccagttga atcagcgcct gttgccaggc actgaccaga tccgtttcat
    30661 caatcgggat cgccaccggc aaattgcgca gcggcagttg tacgccctcc agccatattg
    30721 tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat caccgtggcg cgcacggttt
    30781 gcgccggaaa ctgcacgttc atctcacgca gacgcggatg gtcatgcagc gccgttgcca
    30841 gaagcgggcc gatatcggca aaacagaacg ggtcggcggg ctggtggcga taacattcgc
    30901 ctaccccgcc agaaagcgta atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt
    30961 gcatcaatgc ctgcgcgagc ggcgagagcg ttccgtcaat cacttcgaca atcagttccg
    31021 ccatccggcg ggtaacctgc gccaattgcg cgccggtcag cgaacgggcg tcagtgcctg
    31081 ggccgaagca ctcatccaca atcatctgcc ccggtttatg agcgtaaacc acgcgcccct
    31141 ggctgtcggt ttccagcagg cgaccgccga cgttgaggca ggcagtgccg ctgatttttc
    31201 cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat gtcgatattc agtacccgac
    31261 acagccgttg ttcagaaagg gtttgcgccc cggcaccgtg accggcgatc acggattcga
    31321 gatgcggccc ggcgctggcg acgacaaaat cgcccagcga ccgagagagc gccatcaccg
    31381 ccgggcgagc gttgcgggtt ttcgcgcttt caccggtgat gatgatggca ccagaatcaa
    31441 cgctttccgg cgcaatgccc gcagcctgat attgctcgag gattaaggtt ttcagttccg
    31501 cttcttttaa accaccctgt ttatcgacag gggtaaagaa caccgggctt tgccagctaa
    31561 tttcgcgttt gatgaattcg tagcgcggca cctgcgacac cgccgcacgg ttaaccagct
    31621 ccaggtggga gaagatcacc tgggtggtgg tggtgccgat atcgataccg acgctcagta
    31681 gctggcgagt gttcacgatt gcgcctccgc ttcggtttta gccgcgctcg cgtcttcttt
    31741 tggcaccagc atcatcgcca cgccaattgc cgttacgccg ccgatcaatt tgccgacaat
    31801 catcgggaag atcatggcgt tcatgttggc agcggcgaag cctaaatggt cgcccagcgc
    31861 gaaagcagcg gaaacggcga aggcacagtt gatgactttg ccgcgggtat ccatctgctt
    31921 catcatgcca aacatcggaa tgttgttggc aagcgttgcc accatgccgg ctgccgcgat
    31981 attgttcata ttcagcacct taccgacgct catcagcggt ttttcaaacc agcgagtcag
    32041 cagcagtacc attggatacg cccctaacag aacgcaggag atcgagccga taacttcaat
    32101 ggcgcgcatc acttcaccgg gtttatcgcc aggggccata aagataggat caaggcccgg
    32161 gatcagttcc cagccaagga ggaatttcac taccgcagcg gcaagaccga gggtgatcaa
    32221 tgcaacgagg aatttggcga agatctggaa gccgttgatc attttttccg ggatgaattt
    32281 cagccccagc gccaccagca ccgcaacgat aagtaccggg atcatgttca tcaggatcag
    32341 cgcaaaggtg aattcaacag gctggccgtt gatctgcaca ccggagtaca tcgctaccag
    32401 accaccagcg atacagccaa tcggaatggt cactatgccc gccagcacgc cgagcgccag
    32461 ataacgacgg tcagaaggtt cgataatgcc gagcgccacc ggaatggaaa acacaatcgt
    32521 tggccccatc atcgacccga gaattaaccc agagtatagc cacgctgcta cgtcaccgcc
    32581 cgccagctct ttggcgagga agaagccgcc catatcgcac gccagcagtg ttccggcgaa
    32641 cattgacggg tttgcgccga gcatttcgta aaccgggata atcaccggtc cgagaacgtg
    32701 ggccagtacc ggtgccagtg cggtcatacc gaccatagcc aggcccagag cgcccattgc
    32761 cataaagcct tcttcgaact gaccgccgga tccttcgata cttttaccga acttaccgag
    32821 gaaacgagca gaaccgccga actgcgacag gatcctgtct acggcagcta tcagcataaa
    32881 gaacatcatg atgtacatga tgatttcgtt aattcccatc gcctttactc cctgttagtt
    32941 gttatttatt ggcggatgcg gcgtgaacgc cttatccgcc ctacatgtgc aatcccgaag
    33001 gccagataag acgcggcaag cgtcgcatcc ggcaattgca ccgcgccact ggcggatgcg
    33061 gcgtgaacgc cttatccgcc ctacatgtgc aatcccgtag gccggataag acgcggcaag
    33121 cgtcgcatcc ggcatttatt gcgccgctgc gtacaggccg acaatctgct cgaggctggc
    33181 ggtacgcggg ttactgcgca gacaaatatc ttcctgcgcg gcctgcgccc atgcaccgta
    33241 atgcccggtt gttgcaccca catcagccag ccgcttgttg atcccaactt ccgctatcag
    33301 ctcacttacc gcattaatag cgtcacgatc gtcggatttt ttagttcgca gcgcccgacc
    33361 aatctgacta aaccgatcac gacaaaccat ccggttaaat tccatcaccg ttggcagcaa
    33421 catggcgttc gccagaccgt gcggaatatg cagcgccgcc cctggctgat gcgccatcgc
    33481 gtggcataac ccaagcccgg cgctggaaaa cgccattccc gccatacatg aagccagcaa
    33541 catgctctcg cgggcgacaa ggtcgtgacc gtagcccacc gctttcggca gcgattgacc
    33601 aatcatcgca atggcgccaa tcgccaggct gtcggtaaac ggtgtagcgt tcagcgcgct
    33661 gtacgcttca atggcatggg ttaacgcatc aatgccagtc atcgccgtga catgcgacgg
    33721 cacgccttcg gttagcacag cgtcgagaat cgcgacatcc ggcatcagcg aggcatgggc
    33781 taacacctgt ttgcgcccgc ttaccgcatc gataatcacc gtcacgttgg tggtttcaga
    33841 gccggttccg gcggtcgttg gaatggcaat caacggcagg cgcggttgca gaacgctggt
    33901 ttctgacatt tctgccagcg tgctatccgg gttcgtcacc agcaacgcca cggctttcgc
    33961 cgcatccagc accgagccgc cgccaaacgc gatcaccccg tcacaacctg attcacgcaa
    34021 ctgcgccacg gctgcacaca cgtcggtgat gcacggttcg cccaccggac atggccagag
    34081 cgtcatggcg atacctttaa cggccaggct acgcgtcagc ccagcggtca tccctgcctg
    34141 atgcaaaaag ctgtctgcca tcacgaacag atgtttcaac ccacgcgttt gcgcttgctg
    34201 cccacaactg ctcaccgcgc ccggaccgca aagcgtcacc ggtggaacgc taaatgtttt
    34261 tacccgttgc agattcaggg tatcgaacgc ctgaaagagc gcggtctgca attcattttg
    34321 catatagccc ctccgctttc tctcgcccac tactggcgat tgccagcggg gtcataaaca
    34381 aactgtgctg cggtaaatgc acctgtaacg ccggaaattg tttgcaaaac agcgccgcta
    34441 cgcccggttg catacaggaa ccgcccgcca gccataaatc cgtaatgccc tgcccttcaa
    34501 tatgacgggc cacaatgtcc gccatttttt cgtacaccgg tttcaccgcg ggccaaatct
    34561 cgtcaccgtg accgcgtttg tactgctctg cctcttccag cgaaatacgg cgatttccgg
    34621 cgagagtcag agagatgtga tgtccgccgg tggcttcatc cgccgagtac gtcactttgc
    34681 cctttttcac gatggcaatg ccggtagtgc cgccgccgat atccactaca ccggcgttgt
    34741 ccagttgcag cagatccgca actgcggttg gctcatccag cacatggctc acttccagtc
    34801 cggcagactc cagcacgttg atggaaatac gcgggtccgt accaggagga aatgaggtcg
    34861 ccgcatggct aaaacggcga ccgaattgct gctcaagggt gtcgagatgg cgacgaacaa
    34921 tggtgaccgc accgaagaaa tcccagacga tgccgtcgcg gaccacatcg gcccagtcga
    34981 ggcataccgc caccggctga ccgtcgcggt cgacaaccat cgacaccaca tcgcaggtgc
    35041 ccagatccac ccccagccac agcggagatt ccgtcgcggc gggcgtctgg ttacacagcg
    35101 ttgccgccgt ttgcagacgt ggggtgagcc attgttcatc gtgcgccatc ggttactcct
    35161 taaacaatgc gaaacgcatc gactaataca cagcgacgca gacggacaaa cgtccgcgcg
    35221 ctggttaccc cttcaccggt tggcgtggtg atggtcatcg tggtccagcc ttccccgccc
    35281 agccccagcc cggcaataca cggtccgttc ttaacgaaaa tgctggtatc aatggcgttc
    35341 gccatctggt tcatgttttc gatattgcgc gagtgcattg ccgccgtgtg gtggcaaccg
    35401 ccttccagtt tcaccgccag cgcaatggca tccgccacgt tggcgacgcg cacgacgggc
    35461 agcaccggca tcatcagttc ggtcacggca aacggatgtt ctgcggtggt ttctacaaac
    35521 agcagtcgcg tttgttccgg aactttaagg ccgattgccg ccgcgatttt cgccgcatcg
    35581 cgaccaaccc agtcacggct gacggtgcct tttccgcgct cgtcgatatt tttcagcaac
    35641 accgattgca gttgctgcgc ctgctctgcg gtcagtttca ccgcatgctg gccttccatc
    35701 agacgcatca gttcatcggc tacgctatca acaacaatca gtaccttttc gtcggcacaa
    35761 atgatgttgt tatcgaaaga agcgcctttg acgatggact gagcagcacg ggcgaggtcg
    35821 gcggtttcat ccaccactac cggcgggttg ccagcgcctg cggcaatcag acgtttattg
    35881 gtgtgtttac gcgccgcttc tactaccgct tcgccgccgg tcaccaccaa aaggccgatg
    35941 cccggaaact tgaacaagcg ttgcgcggtt tcgatatccg gatttgttac ggtaaccagt
    36001 aagttttccg gcccgcctgc ggcaacaatc gccaggttga gcagcgtaat cgcccgctgg
    36061 gagacttttt tcgccgccgg atgcggggca aaaatgacgc tgttgcccgc ggcaatcagg
    36121 ctgatggcgt tgttaattac ggttgccgcc gggttagtgg aaggcgtcac tgaagccacc
    36181 acgccccagg gtgcgttttc aatcagggtc gggccgttgt cgccagtcag cacttgcgga
    36241 gagaggcact caacgcctgg tgtgccgcgc gcctgagcga cgtttttggc aaatttatct
    36301 tcaacgcgcc ccatgccggt ttcactgacg gcaagttccg ctaaatctct ggcgtgtttt
    36361 tcgcctgctt cacgaatggc agcaatggct aactggcgca ttgccacgct ttttaaccct
    36421 tgctgggcga ctttggctgc cgcaacggcg tcatccaggg acgcgaaaac gcccatctca
    36481 tgaacggcgg cgggcggcgt gtcactgctt tgcattttca gcagtaccgc tttcaccacc
    36541 tgttcaatat cctgttgatt catgatgttc tgccttattt gtggaaaatg acctgaccgc
    36601 cagacaccac ctcatcgaca atgccaatca cgcacagatc gaccggtgac gtttcgcttt
    36661 tatgcgcctg gcgggcggaa ctgccactca ccagtaacac ccactccccg gttcccgcgc
    36721 caatattgtc gatggcgacg gcgcattgcc cgtcaggatt accttgtgga tcaatcattt
    36781 ccaccatcag caatttgtca tgcgccagtc cgtgatggcg tacggtacaa acaatttgtc
    36841 cagtgacgac tgccagtttc atacccgcct ccgtggcgta tttcaggtaa aagctccccc
    36901 taccctccgc agaaggtaaa atgaaaaagg agagagcgtg acgcccgaat cgacgtcaca
    36961 cagggtgatt acaggttgct gctatcgcct ttcaggccga tcgggaagac ttcttccagg
    37021 tcgccgtgcg gacgcgggat aacgtgtaca gataccagct cgccgatacg ctgtgccgct
    37081 gcggcacctg catcggtcgc ggctttacag gctgcaacat caccacgaac catggcagta
    37141 cacaggccgc cgccaatctg cttaacacca accagcttga cgcgcgcagc tttaaccatc
    37201 gcgtcagaag cctcaatcag tgcaaccagg ccccgggttt cgatcattcc taatgcttcc
    37261 attgtgcttt cctctttatc agggtccaga acgggaccgt tcattcaacc agtgtttgta
    37321 aactgctttc gcggttcact tctgtctgac gcggcacagc tgccaccagc gccagttcga
    37381 taatttcctg cacgctacaa ccacgagaga gatcgtgcat cggcgcggca agtccttgta
    37441 tcaatggccc gacggcacga tatccgccaa gtcgttgtgc gattttgtag ccaatatttc
    37501 cggcttccag cgacggaaaa accatcacat tagctttgcc ctgtaacggg ctggcaggcg
    37561 ctttttgcgc cgccacttcc ggcacgaagg cggcgtcaaa ctgcaactcg ccatcgacaa
    37621 ccagctttgg tgcgcgctca cggacgattt ctgtcgcctg ctggacgttg gcaacacagg
    37681 ggtgacgggc actaccgttg ctggaaaacg acagcatcgc cacgcgcggt tcttctccgg
    37741 tgatggcgcg ccaggtttcg gcgctggcaa gcgcgatatc cgccagttgt gccgccgtcg
    37801 gctgtggcac cacgctgcaa tcggcaaaac ccaacgccgg accgctgtac tgtggcagca
    37861 tcaggaaaat ggatgagagc gttttacagc ctggctgcaa gccgataatg cgtaatccgg
    37921 cacgcagcac attcgccgtg gaagagaggt tgcccgcgat acagacatcc gctttaccgg
    37981 cgctgaccat tgcagcggcg aacatcagcg gatcggtaag tttttccagc gcatccggcg
    38041 gcgttttttc gcccgcgcgg gccagccagc gatgagcaaa ttcttcccgc attgcgaggt
    38101 tgccatgcgg atctatcacc tgtagcccgt ccatcgccac gccgtgactg agcgcaaact
    38161 gacgaagttc aaacggattg gcgaccagaa tgggcgttgc cagaccttgt tgatgtaaat
    38221 attgcgcagc tttcagcaca cgttgatcta acgcatccgg aaaaactact ctggcgggcg
    38281 ctcgcaacgc cagttcacga caacgtttaa taatcattgc gtttccccca gtcgttgttg
    38341 gatctgcttc accgttagcg gctgcttcgt tacgctcagg atcatcatca cgtagaccgt
    38401 gctggaaagg cggttcagcg cctgcaaaat gtccgggcgt aatacttcaa agctgcgggt
    38461 gataaagacc tgcgccgcta cggtttctgt ttcccgcact ttggtgcgta gcagattgag
    38521 caacgccgca tcgcgcccgt ggctggcttc cggcaccaga tgatcgtggt cgagataacg
    38581 cagcggctgg tgagaaagcc gatgcagatc ttcgtcagaa agcccgacaa tcgcttgctc
    38641 gcccagcggt tcccccagcg catcggcacg cataatgttg ccaagacgcg agcggatgtc
    38701 cgccagccac ggctgccacg gttctgccag ttcaatttgc agccacactg ccagcgcaat
    38761 ggtgctgtcg agaacagcgc gaaaacccag acgcggatcg cttttggcaa ccattttttc
    38821 cgccgacagg tgagtcagcg tatccggctt ttttgccacc ggctggcggc acagttcgca
    38881 gcacgcctgc ggatgttcat cgctactggt cagcccgtga acgggctgcg gctgctgctg
    38941 ttcatcgtca acaaacaggc ggccctgctc gtcaataaac ttgatgcgca gatggcggct
    39001 ttccagtaac tcccgggcag agggcgtcag gcgactgtcc gcagggagat ggatctctgc
    39061 gccttcgctg agcgtatggt tcgctcttag ccatgcttcg gtgatgaaat ctttcataag
    39121 gattgccagt tagccggcca ggcgacatac aggaatttca cgctggatgt cgtaccaaat
    39181 tcgatgctgg agcctttcgg gataaacatc acatcgcccg ctttggcgat catagtttcg
    39241 ccttcgtggc gaacatgcag ctccccttcc agcaccatgt cgatttcgtc gtagttcagg
    39301 gtccacggaa agaaggcgtt ctcccactgc atgaaaccag cggccatact gctgccgtcg
    39361 tctccggtca cgagatcagt caagcctacg cagtgcggtt ccgcgccatc gaaacgacca
    39421 aacttgacgc tgctgccatc aataactttg atgccgcctt tgccggtcac cgatttaaag
    39481 ctcggctgca gtgcaccctg ttccagcgac tgtttttcct tcatcacttt ttccatcagc
    39541 tgcgcgacca ggctttcggt aaactgacct tccggcagtt gggcgataat ggtttcacgg
    39601 atgcgctggc tttcggtttt atcggctgat gcgctggcgg gaacagacgc cgttaccggg
    39661 atcgattcat cacattcggt gatggtaaaa cccaacagat cagccacttc acgagcttcc
    39721 ggggtaataa tgctggcgcg cagaaccacg gacattgcca gttcgccgcg cgcgtgtgct
    39781 tcacgaatat cattcgctgt gatgagtttt ttcacctgct tcctcctgtt tggtgagaga
    39841 tgccagataa tccaccagtt gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat
    39901 cggctcttcg aagcccgtct caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg
    39961 catgtccgtt ttactgatga cggcgatttg ccgtttgctg acgccaatat ccagcaaccc
    40021 ggcaggcaac cgactttccg ggtcattcgc gccgtggaca tagatcagca tatccacatc
    40081 ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg ctaaaatatt cacccggcgt
    40141 atcaatatcg ccattatcat taaattccac ggcctgtgtt tttcttgcga gggtataatt
    40201 cccctgcaac gcattaaata acgttgtttt tcctgccccg acggtaccca caaacgcaat
    40261 tcgtttcatg aatgcctcgt attaactttt ggtcatttca caaagcgtat aatttaataa
    40321 gcgtcctaaa ccactgacgg tttgtgataa cgcttcttct actgcgccca ccgagccata
    40381 aatcaccagc gcgccactaa aacgatcgag aaaaccgata tgcacatcgg cggctttcaa
    40441 cgctaaatca ccggcgatca tcgccgtttc tccgggagtt agcgtcataa tgccgattgc
    40501 gcccgcatcc ggaacgccga ttttcttcgc cagttcctcg ccgggatgcg cgatgagatg
    40561 cgccagtgtg acctgtttac cgggcacaaa ttcctgaatg atgcgttctt tatccatgag
    40621 tcgccgcctg tgaaaaactt tcgtgtatcg tgacaatttt tcgtcagagt aaataacaaa
    40681 attcggcaag cgcgtttaaa aggtgaggta gatcactaag aatgga
//
LOCUS       DACADA010000024        40475 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.024, whole genome shotgun sequence.
ACCESSION   DACADA010000024 DACADA010000000
VERSION     DACADA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.024"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(14..295)
                     /locus_tag="ISB17_001263"
     CDS             complement(14..295)
                     /locus_tag="ISB17_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAP1979216.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(558..1667)
                     /gene="apbC"
                     /locus_tag="ISB17_001264"
     CDS             complement(558..1667)
                     /gene="apbC"
                     /locus_tag="ISB17_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAP1979217.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1799..3832
                     /gene="metG"
                     /locus_tag="ISB17_001265"
     CDS             1799..3832
                     /gene="metG"
                     /locus_tag="ISB17_001265"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP1979218.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3973..7773
                     /locus_tag="ISB17_001266"
     CDS             3973..7773
                     /locus_tag="ISB17_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HAP1979219.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPLMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            7786..11547
                     /locus_tag="ISB17_001267"
     CDS             7786..11547
                     /locus_tag="ISB17_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HAP1979220.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            11557..15189
                     /locus_tag="ISB17_001268"
     CDS             11557..15189
                     /locus_tag="ISB17_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAP1979221.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15250..15566
                     /locus_tag="ISB17_001269"
                     /pseudo
     CDS             15250..15566
                     /locus_tag="ISB17_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16268..17356
                     /locus_tag="ISB17_001270"
     CDS             16268..17356
                     /locus_tag="ISB17_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP1979222.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17367..19646
                     /locus_tag="ISB17_001271"
     CDS             17367..19646
                     /locus_tag="ISB17_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979223.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            19639..20775
                     /locus_tag="ISB17_001272"
     CDS             19639..20775
                     /locus_tag="ISB17_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1979224.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRYSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20772..22775
                     /locus_tag="ISB17_001273"
     CDS             20772..22775
                     /locus_tag="ISB17_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979225.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22900..23361
                     /locus_tag="ISB17_001274"
     CDS             22900..23361
                     /locus_tag="ISB17_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAP1979226.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23402..23872)
                     /locus_tag="ISB17_001275"
     CDS             complement(23402..23872)
                     /locus_tag="ISB17_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP1979227.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23919..24638)
                     /gene="yehT"
                     /locus_tag="ISB17_001276"
     CDS             complement(23919..24638)
                     /gene="yehT"
                     /locus_tag="ISB17_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAP1979228.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24635..26320)
                     /gene="btsS"
                     /locus_tag="ISB17_001277"
     CDS             complement(24635..26320)
                     /gene="btsS"
                     /locus_tag="ISB17_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAP1979229.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26542..27273
                     /gene="mlrA"
                     /locus_tag="ISB17_001278"
     CDS             26542..27273
                     /gene="mlrA"
                     /locus_tag="ISB17_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAP1979230.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27333..27440
                     /locus_tag="ISB17_001279"
     CDS             27333..27440
                     /locus_tag="ISB17_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAP1979231.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27421..28152)
                     /gene="yehW"
                     /locus_tag="ISB17_001280"
     CDS             complement(27421..28152)
                     /gene="yehW"
                     /locus_tag="ISB17_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAP1979232.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28157..29083)
                     /gene="yehX"
                     /locus_tag="ISB17_001281"
     CDS             complement(28157..29083)
                     /gene="yehX"
                     /locus_tag="ISB17_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAP1979233.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(29076..30233)
                     /gene="yehY"
                     /locus_tag="ISB17_001282"
     CDS             complement(29076..30233)
                     /gene="yehY"
                     /locus_tag="ISB17_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAP1979234.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30240..31157)
                     /gene="osmF"
                     /locus_tag="ISB17_001283"
     CDS             complement(30240..31157)
                     /gene="osmF"
                     /locus_tag="ISB17_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAP1979235.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31347..33644)
                     /gene="bglX"
                     /locus_tag="ISB17_001284"
     CDS             complement(31347..33644)
                     /gene="bglX"
                     /locus_tag="ISB17_001284"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAP1979236.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            33840..35555
                     /gene="dld"
                     /locus_tag="ISB17_001285"
     CDS             33840..35555
                     /gene="dld"
                     /locus_tag="ISB17_001285"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP1979237.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35594..36526)
                     /gene="pbpG"
                     /locus_tag="ISB17_001286"
     CDS             complement(35594..36526)
                     /gene="pbpG"
                     /locus_tag="ISB17_001286"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAP1979238.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36700..37287)
                     /locus_tag="ISB17_001287"
     CDS             complement(36700..37287)
                     /locus_tag="ISB17_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAP1979239.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37457..38035
                     /locus_tag="ISB17_001288"
     CDS             37457..38035
                     /locus_tag="ISB17_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1979240.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38165..38926)
                     /locus_tag="ISB17_001289"
     CDS             complement(38165..38926)
                     /locus_tag="ISB17_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1979241.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38979..40415)
                     /gene="mdtQ"
                     /locus_tag="ISB17_001290"
     CDS             complement(38979..40415)
                     /gene="mdtQ"
                     /locus_tag="ISB17_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAP1979242.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT        10063 a        10771 c        10193 g         9448 t
ORIGIN      
        1 ccagggtggc aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctatctg
       61 cagcaatcac ttccgtcgtt acacctttaa ctggcgaaga aacctgatga cgggtatcaa
      121 cattatacgt taactgacca caatgctgtt gaggttgacc attaaccata gccatactgc
      181 atgtcggggg actgatccta ccattaatcg ttaatgtggc agtttctgaa gagaacgcgg
      241 gagaagccaa tccataggca agaaaaataa ttcctgacag ccagtactta ttcattgaac
      301 gttatccctg tagtaaaggt tatgcctgga aagaatgagt gcataacaaa ctatagctgt
      361 acatccacta cacagccacg aaggatgata atgaagcatc gccggtacga tcaatcgact
      421 ttgtagagtt tcggacgaag gtccgcagaa tattcgcaac attaaataag tgttcattaa
      481 tgttgcaaaa acaaatacac cgcatcaatg taacatctct gcggtgtatg aagaataacc
      541 agaatggtta atacgcgtta gaccgcgcgg aaggaaatct cgccaggaat gacttcaccc
      601 tgccagtaga gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg
      661 ctctcaggac ggctgataac cgttggcgtg cctttatcga gatcctcacg taaggagatg
      721 tgtaacggca tctgccccag cagctgcgtg tggtatttct cggccagttt ctctgcacca
      781 ccggtaccga aaatcggctc atggtgacca cagttactgc aaatatgcac actcatgttc
      841 tcgacaatac cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca
      901 tcgatcaacg cgatgtcctg cggggtggtg accactaccg cgccagttac tggaatgttt
      961 tgtgccagtg tcaattgaat gtcaccggta cctggcggca tatcaagcac cagatagtcg
     1021 agatccggcc acaaggtttc ctgcaacatt tgcattaacg ccttgctcgc cattgggcca
     1081 cgccacacca tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca
     1141 tgagacataa tcggtgccat atgggtaccg tccggcgaag ttggacgctg attttctgcg
     1201 cccagcatgg tagggattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc
     1261 ccttctgccg ccagtgccaa cgccaggttt accgccgtgg aggatttacc cacgccacct
     1321 ttaccggaac tgaccgcgat aatgttcttc acgccgttaa ttcccggctg attcttcacg
     1381 cgtttcagag tggctatgtt atgcgacagt ttccagtcga tagctttcgc tccggtgatg
     1441 cgcagcagtt cggcgctaca ttgctctttt aactcttcga acgcgctatg ccagacgaat
     1501 ggcatgacca gttccacatg cagcgtgtcg tccatccagg cgacatggtg caacgctttc
     1561 agcgttgtga ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc
     1621 attgcgcgca aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt
     1681 ttgtatagat aaaccatcag ctgatagttt acctgaagaa tatagagaag tacttactta
     1741 acattttccc atttggtact atctaagccc ttttcactat taagaagtaa tgcctactat
     1801 gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat
     1861 ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat
     1921 gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc cacggcacgc cgatcatgct
     1981 taaagctcag cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggaaca
     2041 tcagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga
     2101 agagaatcgc cagttgtcag aacttatcta ctctcgtctg aaagaaaacg gttttattaa
     2161 aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt
     2221 tgtaaaaggc acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt
     2281 ctgcggcgcg acctacagcc cgactgaact gatcgagccg aaatcggtgg tttctggtgc
     2341 taccccggta atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat
     2401 gctgcaggca tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga
     2461 gtggtttgaa tctggcctgc aacagtggga tatctcccgc gacgcgcctt atttcggttt
     2521 tgaaattccg aacgcgccgg gcaaatattt ctatgtctgg ctggacgcac cgattggcta
     2581 tatgggctct ttcaagaatc tgtgtgacaa gcgcggcgac agcgtaagct tcgatgaata
     2641 ctggaagaaa gattccactg ccgagctgta tcacttcatc ggtaaagata tcgtttactt
     2701 ccacagcctg ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgaccaacct
     2761 gtttgttcac ggctatgtga cggtgaacgg cgcgaaaatg tccaagtctc gcggcacctt
     2821 tattaaagcc agcacctggc tgaatcattt tgacgccgac agcctgcgct actactacac
     2881 cgccaaactg tcatcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg
     2941 cgtgaatgcc gatatcgtga acaaagtggt taacctggcg tcccgtaacg ctggctttat
     3001 caataagcgt tttgacggtg tgctggcaag cgagctggct gacccgcagt tgtacaaaac
     3061 cttcactgat gccgctgaag tgattggcga agcgtgggaa agccgtgaat ttggtaaagc
     3121 tatccgtgaa atcatggcgc tggctgacct ggctaaccgc tatgtcgatg aacaggctcc
     3181 gtgggtggtg gcgaagcagg aaggtcgcga tgccgatctg caggcgattt gctcaatggg
     3241 catcaacctg ttccgcgtgc tgatgactta cctgaaaccg gtactgccga aactgaccga
     3301 gcgcgcagaa gcattcctca atacagaact gacctgggat ggtatccagc aaccgctgtt
     3361 gggccataaa gtgaatccat tcaaggcgct gtataaccgc atcgatatga aacaggttga
     3421 agcactggtg gaagcatcca aagaggaagt aaaagccgct gccgcgccag taactggccc
     3481 actggcagat gatccgattc aggaaaccat cacctttgac gactttgcta aagttgacct
     3541 gcgcgtggcg ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct
     3601 gacgctggat ctcggcggtg aaaaacgcaa tgtcttctcc ggcattcgtt ctgcttaccc
     3661 ggatccgcaa gcactaattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa
     3721 aatgcgcttc ggtatctccg aaggcatggt aatggctgct ggtcctggtg ggaaagatat
     3781 cttcctgcta agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt
     3841 caaggcgctg catcgacagc gccttttctt tataaattcc taaagttgtt ctcttgcgat
     3901 tttgtctctc tctaacccgc ataaatactg gtagcatctg cattcaactg gataaaatta
     3961 cagggatgca gaatgagaca ctttatctat caggacgaaa aatcacataa attctgggcg
     4021 gtggagcagc aaggcaacga gttacatgtc agttggggca aagtcggcac taacgggcaa
     4081 aaccaggtaa aaagctttgc tgatgctgcg gcggcggaaa aagcaaagct taagctgatc
     4141 gcagagaaaa cgaaaaaagg ctatgtggaa aatgcttctg caaacgtgca tatcaccccc
     4201 atcaccaaag ccactcctga agttgaaact tctcctgaga gtaaaaacca acgcccctgg
     4261 ctggctgatg atgcggtcat tcggctaact gacgatatta atcgatttgc ttttcctcat
     4321 cgctctcgcc ccagagagat caattatctg cacaaagata gtcaaatatg gcagcgcatc
     4381 gcgaataaca ccagggcata tgatcccgcc aacaattacc gttcttatcc agaaaactgg
     4441 cagcaggctt ttgctgagtt acaaaggcga attctggata atcaacagac agggagtgcg
     4501 caatctgatg cggcattgct ctggagtttc tggaattctt actctgcaga tgaactagtc
     4561 gatgacttaa tcattcgctg tggtatggaa agtgccgttg aaattgccct tctcgctctt
     4621 caactgagat ataaaccagt aaaaggcgca gtaaccacca tcattccacc taatcacgaa
     4681 gcagaatcgc taccgagttg gcatcaacgc ctatgtcatc atctttcact cgcttcagaa
     4741 gatgagtggc aacgctgtgt agataaagta ctcgcggcta ttacgacgct atccccagca
     4801 cggcaacctt ttgcggcgtt actgctccct gaacgcccgg atattgccaa tgctatggct
     4861 ctacgctatg cagatcaaaa cgttccggcg atgacctggt taagactggt agtaaccgat
     4921 gaagccgctc tgactgcagc cgaaaaatac gactttccac cgttatatcg tgattttcgc
     4981 gcttatctgg caatgttgct ggcaaataat ggggtgagcg gtgtaagccg gatacttact
     5041 gagtttgtgg aagagcattc tgacaatccc acttatctct tcgagcgcat cagcgagact
     5101 gaagatcttg tcaaatggtt atggaaaacg aatcacccgg atgcgattca aattctgatc
     5161 ctcggtgtaa ttggcaagaa aaagcacctg gaatacttaa gcaaagcatg tcaaaaacat
     5221 cccgctgcgg ctattgccgc ttatgcaact ttgctggcaa tacatgaaga taaagagtgg
     5281 cataaagcgc ttgtcaaatt gattaccatc acgccagagt tagtatgcga cgtcattccc
     5341 tgggttagtg ctaaagcggc aggcatctta tctgaatgtc gtcctcagtc agtagctgaa
     5401 gaatgtgagt atgccactgc ggatatgttg ccagagctgt ttgtttcgcc gccctggatg
     5461 actaaagaga aaaagaaaaa ttcgccggtg tttgatcttc ctgtactgcc agttccttct
     5521 gtttctgacg tcactccaga gattgccaaa aaactaaccc gtacatacct tgtcacccgc
     5581 ttccagcaaa tagcccaaca gcaggcaaca aaacaaatat tgtttaccga cctgccgctg
     5641 atggaaaaag caagctggga gagacactta gtccccctca caccagaaca gcaaattctg
     5701 tggtgtctgg gttttgacaa atggcgggag agcggagaaa aaatatacga aaaaattccg
     5761 gcgccacaaa gtgccgttga tgccctgcta cgcttcgatt ttccagcact caatgctgaa
     5821 tttgcgcaat accataacaa tgcttacaaa agctggaatc tcattgccct ctgttatcta
     5881 cctggtcaac aggccatttc ttttcttaac cagatagtca aagaagataa ctactctggt
     5941 gagggatata ttctggccat ttttggtagc gccgccattc ctgcatttat gacctgcctg
     6001 caacgtgagc cacaacggtt gtggtttttc accttcttcc ttggtgtcaa tgaactggca
     6061 ttgccaatgg cgcaacgact tcagaaaaaa atgtcgtatg aagatgcacg aaactggcta
     6121 acggattacc cacgccatgc cgccgccggg ctacttcctg tggcactggg caaacaagga
     6181 aaagatcgtg attgtgcccg ccaggcgctc cgtctgctgg tgaatttaaa ccagcgagaa
     6241 acgattgagg aaatcgccca aggatacaac caacctgatg ttttagctgc gctggcaaca
     6301 ttatttgata gcgagccgct ggaagaatat cccgctaaaa tcgccccact gcccggcttt
     6361 tatcagttca ccttatggcg cagaccacgg cttaaaagta acaaccagcc tctgccagat
     6421 gacgctatgc gccacctcgg cataatgctg agctttccac gcgacattac cccctacgca
     6481 gggcttgcga tcatcaaaga aaccttcacc cgcgaatcac tggctgaatt tggctgggat
     6541 ctgtataccg cctggacaga agctggcgca cccgcaaaag aaaactgggc atttacctcg
     6601 ctggggattt taggcaatga cgacaccgcg cgtaaattaa ccccgctcat ccgcacctgg
     6661 ccgggtgaat cccagcataa acgcgcggtt tacgggctgg atgtgttagc cagtatcggt
     6721 agcgacattg cgctaatgtt gcttaatggc attgcgaaaa agattaaatt cgttgcatta
     6781 caggaacatg cctgcgacaa aatcaacatg gtcgctgaaa atcgcgggct gactatggct
     6841 gaactggaag accgcctggc accggattta ggacttgata gcagtggctc gttgacactg
     6901 gatttcggcc cacgtcaatt taccgttggt tttgacgaaa ctttaaaacc cgtggttcgc
     6961 gacgcaaacg gcaaagttct gaaagattta cccaaaccaa atcagagcga tgataaaacg
     7021 ctggcaactg acgcggttaa tctcttcaaa cagttgaaaa aagatgtacg cgccatagcc
     7081 agccagcaga ttgatcgtct ggagcaggct atgtgccagc gcagacgctg gacggcagag
     7141 cagttccgcc tgttcctggt ggagcatccg ctggtacgtc acttaacccg acggttgtta
     7201 tggggggtat atactgagga aaacacgctg attgcctgct tccgggtcgc agaagacagc
     7261 acttacagcg atgcgcagga tgagctattt acgttgccag caggaaacat cggtattccg
     7321 catgtgctgg aaatatctcc tgaatccgcc gctgcatttg ggcaaattta tactgattac
     7381 gaacagcttc ctccgttccg ccaactcgat cgtggttatt accaccttgc tgataatgaa
     7441 cgtgattccc atgaattgat acgctggcaa ggacgtcttt gccaggccgg gcgcattgtc
     7501 gggctggaac gccgaggctg gcaacgtctg gaagaaagcg gcagcgttta cgcgatgcgc
     7561 aaatctactc cttatggcga tctcgaactt gaaacagaac ctttttcatt aatttatggt
     7621 gaaacggggt atggcgacca gctaccggtt gaaagcgtaa aaatcacctc gcctgataat
     7681 cgctacggta aacaatcctc actcactttt tccgcgctcg acgacatcac cgccagcgaa
     7741 ctcattaacg acattgaatc actgtttgat taaaaggatt tagcaatgaa aacctatatt
     7801 tatcaggacg aaaaatcaca taaattctgg gcagtagagc agcaggaaaa tgaactgcat
     7861 atcagttggg gcaaagtcgg cactaacggg caaagccaga taaaaagctt tgctgatgcg
     7921 gcggcggcgg aaaaagcgga gctgaagttg attgcggaga aaacgaaaaa gggctacgca
     7981 gaagaagtat ccgtgacgac accgacatct gcccccgtcc aggttatcgc atgctctgaa
     8041 aaagcccctt tgccgcaaga caaaccacca tttgctgaag accaccttcc ctggctggca
     8101 gacgacgccc aaatcatttt accgacagag gtcgcaccaa ccacactaag tcatcgacgc
     8161 tggcctggcg attctgtgcc acaggagaat gaattaactc tgctacgctc cgtcgccgcg
     8221 catactcacc ggcgttttaa aaaggtgatc accttcgatt acagcacctg ttcactggac
     8281 tggcagcagg cgattacaca agcagtgggc cttatcgact caccgattag caccgcacta
     8341 ccaccgatgg ttctggcggt actggttgcg ctggaacaag gcttcaatcg caacgatcac
     8401 gaagagttaa tggatcaaat tgtccaggag ggtggtctgg aatatgccac tgaggttgtc
     8461 attgcattgc aagccatccg ctttgactgg aattatgatg cccacctcat tacgtttacc
     8521 ccggataaca agcagcctgg ctatctgtca cgctttgcct cggttgaaat gcgtctgcgt
     8581 aaacatctct cactagcaaa tgacgatgta tggcagcgct gcgccgacaa actgattgca
     8641 gctctcggaa acatacccgc ctggcatcag ccgctggttg cgttattgtt gccggaaaaa
     8701 caagatgtga gccatgagat tgccagacgt ctttgcggac aaaaagggtt atatgcgctg
     8761 gaatggctaa aactaacggc cgccgacgag caggtattgg cagaactggg gaaatactat
     8821 ccaggacagc cagggcaggt atttgatgat tattacggcg gtaaaatctg gtgcgcaacg
     8881 atactcaagg agcaaggtgt cggtgcgctg gcccgttttg ctccttacgc tgctggtgac
     8941 acatgtggtg aagtgttgat gcatatcaac cacccgcagg cacttacact acttatccat
     9001 gcaagcgagc agggaaagcg ttgccacgat cgcatgacaa agactttcgt tcgctttcca
     9061 catgcagcgc tggcggcatt agcagaactt ctggcacaaa aagatcaaaa gcgttggcgc
     9121 atgatgctaa tgacaatgct tatctcacaa ccaacattag cagagcgagt aatcccctgg
     9181 ctctcaacac ccgcagttgc cgtactgaaa tcatgccagc aacaactgac acagccctca
     9241 aaccatgcca gcgctgatat gctgcctgcc gtattagtct caccgccctg gctttcgaaa
     9301 aagaaaaaat ctgtaatgcc ggtattggat ttaacgccat taccgctgga gtcatgctgc
     9361 acattgaccg agacggcaga aaaagagatt catgcaaggc atcgctggca cgcacatcaa
     9421 atagacatcg gccaaaaaga ggatattcag aactacctga cacgtctggg tttcaatcgc
     9481 tggaataatg gccagtatat gaaagcgtct gatgctgttg tagaactctg gcagagggga
     9541 gattattctg cgctaatcag cgaatttaaa accttctggc actcctatca acgggaatgg
     9601 cagctttaca tgttagccgc cctgcctatc gaaaaaacag cgcaggcgtg gaatgtcctc
     9661 agtaaagagc cacacgtcgg tgtcgagttt gtcatgacgc acctgcaact cgctgggtta
     9721 cagggtttta ttcactcctt ttcacgctat ccacaagaag ctctgcctgt agcacaatat
     9781 tttgcggcta tcgaattagc gccactgatc gcccgcgcct tcaacaaact caaaacatta
     9841 cgacaagacg cccgtatctg gctgctgaaa tacccggaac acgccattac cggcctgctg
     9901 cctgcagcgc tcggcaaaac cggtgaagca caggacaacg cccgtgctgc cttgcgtatg
     9961 cttaccgaaa acggtcatca gccattgctg caagaaatcg cccggcgtta taaccagccg
    10021 gaagtaaccg atgcggtgaa cgctctactt gcgctcgatc ccttagataa tcacccgaca
    10081 aaaatcccca ctcttccgac cttttatcag ccatcgctct ggacgcgccc gttattaaaa
    10141 gcaaacgccc aatcactgcc tgatagcgca ctcctccacc tcggtgaaat gctccgcttc
    10201 cctcaggaag aggcactgta tccaggatta ttgcaggtga aagatgcctg taccaccgac
    10261 tcactggctg aatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg
    10321 aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa
    10381 ctgacgccat taatccgtgc ctggcctggc gaatcccagc ataaacgtgc caccgttggg
    10441 ttggatatcc tcgccgctat cggtagtgat atcgccctga tgcagcttaa cggcatcgcc
    10501 cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaatagc caacattgcc
    10561 gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg
    10621 gatgataacg gttcgttgct gctggatttc ggcccacgcc agttcaccgt cagctttgat
    10681 gaaaccttaa aaccgtttgt gcgtgacgct tccggcagtc gtctgaaaga cctgcccaaa
    10741 ccaaacaaaa gcgatgatga atcacaggcg aacaatgctg ttaaccgcta caaattgctg
    10801 aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa ggctggaatc cgccatgtgc
    10861 ctgcgtcgcc gctggtcacc ggaaaacttt cagctcttcc tggttgagca tccgctggtc
    10921 cgccacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct
    10981 tgctttcgcg tagcggaaga taacagctac agcaccgctg acgatgatct tttcaccctg
    11041 ccggaaggcg atatctctat tggtattcct cacgttctgg aaatatcacc gacggatgct
    11101 gccgccttcg ggcagctttt tgccgactac gaactgttac caccgttccg ccagcttgat
    11161 cgtaacagtt acgcgctgac agaagccgag cgcaatgcca gcgaactgac acgctgggca
    11221 ggcagaaaat gcccgagtgg tcgggtaatg gggctggcaa ataaaggctg gatgcgtggt
    11281 gaaccgcagg acggtggctg gatcggctgg atgatcaaac ctctgggccg ctggtcgtta
    11341 atcatggaaa tcgatgaagg ttttgcggtc ggcatgtcgc cagccgaact cagtgccgag
    11401 caactcttaa gcaagctgtg gctatgggaa ggcaaagcgg aaagctatgg ctgggggagt
    11461 aattcaacac aggaagcgca gttctccgta ctcgatgcca tcaccgccag cgagctaatt
    11521 aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg
    11581 gcggataacg cccaactgga actgaaatat aaaaaaggca aaacaccact cagccatcgc
    11641 aattggccag gcgaaccggt acctgttatc actgaaaaca tcattcagac actgggtgat
    11701 gaattgctac aaaaagcaga gaagaaaaaa aacattgtct ggcgttatga aaacttttca
    11761 ctggagtggc agtccgccat cacgcaggcc atcaacttga tcggcgaaca caaaccctca
    11821 atcacggccc ggacaatggc ggcgctagcc tgtatcgcgc aaaatgatag ccaacagttg
    11881 ctcgacgaaa tcgtccaaca agaggggctg gaatatgcaa ctgaggtggt gattgcacgc
    11941 cagtttattg tacggtgtta tgagagtgat cctctggtag taacattgca atatcagaac
    12001 gaggactatg gctatggtta tcgctcagaa acctataacg aattcgatct ccgattgcgt
    12061 aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc
    12121 gcactgccag gaataaccaa agttcgccgc ccttttattg cgctgaacct cccggaaaaa
    12181 cccgaaatcg ccaacgagct ggtaagcctt gaatgttcgc aaactcattt tcgctcaaaa
    12241 gagtggttaa aagttgttgc tgatgaccct aaggcggtga aagaactcgc acgctactgg
    12301 agccaggata tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc
    12361 tacgcggcct gcgctgccct tttgcgcgaa caaggactgg cagccgttcc gcgcctcgcg
    12421 atgtatgcac ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc
    12481 atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg tgtggctaaa
    12541 tacagtaaaa acttccccca tgcgacgctc gccgcactgg cagagctgct ggcgttaaaa
    12601 gccccccctg cgcgccctgg ttatcccatc atcgaagaca aaaagctgcc tgcacagcaa
    12661 aaagcacgtg atgaatactg gcatacgctg ttacaaacgc tgatggcatc gcagccacaa
    12721 ctggcagaag aggtgatgcc gtgcttatgt acacaagccc aggcagtggt gaatggttat
    12781 ttatcggcat cgccaaaact tgctttcgag agtactcata gcaacgacct gccagaaata
    12841 cttgtttctc caccgtggcg aggtaagaaa aaaacggcat tgctgcgcct tgatttagtg
    12901 cctcttgaat tagcgcccaa agcccgctgg caaccaggcg aacgagagag acttgccgcc
    12961 acagagtctg cccgttattt cagcacggga tcttttacgg aacgcatgga acgaaaaagt
    13021 ggtcgagttg tactacagga gctaggtttt ggggatgatg tatggctgtt ccggaattat
    13081 atactgcccg gcaaactgga tgcggcgcgc aaatcattag tgggtcagtg gcattactcc
    13141 ccgcgcagag tagaggaaat caacaatggc tggcactcca cggaagcaaa atcagccgaa
    13201 caggcgcttc gttccggcga cgttgaagcg ttaattaaca cctgggaaaa tgatagctac
    13261 tcgcattatc gtcaggaaaa gagtgtctgg aacctgtatt tattggcgca gttgccacgt
    13321 gagatggctt tgactttctg gctgcgtatc aatgagaaaa agcatctatt cgcgggtgag
    13381 gactattttc tcagtatcct cgggttggat acattacctg gcctgttatt ggctttttca
    13441 catcatccaa aagaaacatt tccgttaatt ttaaatttcg gcgcaacaga actggccctg
    13501 cccgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg ccagtggatt
    13561 ttacaatggc cggaacatac ggctactgca cttatccctc ttgtctttac aaaacccagc
    13621 gataatagcg aagctgcatt acttgccctg cgtttactgt atgaacaagg gcatggcgaa
    13681 ttactgcaaa ccgtggcaaa ccgctggcag cgtacagatg tatggcctgc cctggagcaa
    13741 ttacttaaac tgggtccaat agaaatttac ccggcacgta ttccaaaagc tcctgatttc
    13801 tggcatccgg gaatgtggtc caggccgcgc cttatcacta ataatcagcc tgttaccgat
    13861 gacgctctgg aaattatcgg cgaaatgctg cgctttaccc agggcggacg tttttatggc
    13921 ggactggaac agctgaaaac gttttgccag ccgcaaacgc tggcagcttt tgcctgggat
    13981 cttttcaccg cgtggcaaca agctggtgcc cccgcaaaag acaactgggc atttctggcg
    14041 ttaagtctct ttggtgacga aaccacggca cgagatctga cgacacagat cctcgcctgg
    14101 ccacaagaag gcaaatctgc ccgtgctgtc agtggcctga acatccttac cctgatgaat
    14161 aatgatatgg cgctgataca gctgcaccat atatcccaac gggcgaaatc ccgctcctta
    14221 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa
    14281 gagttagcgg acagattagt cccaacattg ggccttgatg atccgcaggc gttgagtttt
    14341 gattttggtc cccggcagtt taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt
    14401 gatcagcaaa acgttcgcca gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg
    14461 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgccac tcaggtgagc
    14521 aaaaacctgc tcccacgtct tgaagcagcc ctacgtatca cccgccgctg gtcgctggca
    14581 gattttcatt ctctgtttgt taatcatccc tttacccgcc tggttaccca gcgattaata
    14641 tggggggttt atccggcaaa tgaaccgcgt cgtttactca acgcctttcg tgtggccgca
    14701 gagggggagt tctgcaatgc acaagatgag cccattgacc tgcctgcgga tgctctgatt
    14761 ggcattgccc acccgttaga aatggcagca gaaatgcgca gtgaatttgc acagcttttt
    14821 gccgattacg aaattatgcc gccttttcgc cagttggcgc ggcgcacggt gctgctcaca
    14881 cctgacgagt cagccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg
    14941 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    15001 gacttaggtg cgtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    15061 gatagcaaag cgctaatgag cttccgttct cttcgggttt accgtgacaa taaatccgtc
    15121 acttttgccg aacttgatgt gtttgatttg agtgaggcat taagcgcacc tgacgtcatt
    15181 ttccattaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cctaccagcg
    15241 ggggaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga gatccctgta
    15301 cctgaagagc cgttttcctt cagacttgaa atccataaat gctctgaatt atttacaggt
    15361 tccgtctatc gactggagcg attccggcta catccaacat ttcatcaacg tgatcgagaa
    15421 gatgccgacc actaataaat gatgcgttga tttatataag agatgagttt attgatgagc
    15481 ggaaattacg aggtgaatca catgaaacag taatagcaat ttttaattgt gaactacaga
    15541 atatattcaa ccaagaaata gaataatata ctctagataa ttcaagtcgc aggaaggcga
    15601 taagcgagtg agtccccagg agcttactaa agtaagtgac aggggtgaac gaacgcagtc
    15661 gcagcacatg caacttgaag tatggcgagt atagctttat taaaggggga aacggtattc
    15721 ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc
    15781 cactaatagc tccagtacag caatgagcac ggttaacttt tgtgtcagcg tgtccatccg
    15841 ctctcctttt aaggagcgca acaccagcac cttaccctta cactgcctgt cgatactggt
    15901 ttacgtatgt tggaccacga tgttgcactc cggatcaggc accacctgca gcaccacccg
    15961 tccggtagcc aggacttgaa attggttcga tccggcgcaa cctcccaaca gtcaggatta
    16021 taaatctcat tatcttaatt gcaacggggt ccagcggtgg ttataattcg ttcgttggaa
    16081 cacgatgttg cacaggctgc ggtgttaggc ctgaaagtaa taaataaaag aacctgcctc
    16141 accagcaggt ttttttattt tttgcgatct gctttccaga tattttccgc tcaaacaact
    16201 aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac atttcaggga
    16261 tgaatatatg tcaccgcaga ataatcacct gcagcgtccg cctgctgctg tgttatacgc
    16321 cgttgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg gttggcagtt
    16381 aagtttgcct gcggcccgtg cttttattct tggcgacgaa gcgcaaaata ttcgccgtaa
    16441 agtggtgatt agtccctctg ctgtcgaacg catgttagtc actcttgcca ccgggcgcgg
    16501 tttgatgttg gtgggggaac cgggtaccgc aaaatctctc ctgtctgaat tactggcaac
    16561 cgctattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacca ccgaagatca
    16621 aatcaagtac ggctggaact acgccctgct tatcaatcat ggtccgtcaa cggaagcgct
    16681 ggtccctgcc ccgctttacc tgggaatgcg cgacggcaaa atcgtccgtt ttgaagagat
    16741 aacgcgtact ccactggaag tacaagactg tttattaggt atgctttctg acagggttat
    16801 gacggtgccg gaactcactg gtgaagccag tcaactctat gctcgggaag gctttaatat
    16861 tattgccact gccaatacgc gcgatcgcgg cgtcaatgaa atgagtgccg cgctaaagcg
    16921 tcgcttcgat tttgaaactg tctttccgat tatggatttc gcccaggagc tggagcttgt
    16981 cgccagtgct tcggcgcgtt tgctggccca tagcggcatt ccgcataaag tgcctgacgc
    17041 ggtactggag ttactggtcc gcacctttcg cgatctgcgt gccaacggcg aaaagaaaac
    17101 gtcaatggat acactgacag cgattatgtc caccgctgaa gccgtgaacg tggcccacgc
    17161 ggtgggcgtc cgcgcctggt ttttagcgaa tcgagcaggt gagccagccg atctggtgga
    17221 ctgtattgcc ggaaccatcg tcaaagataa cgaagaagac cgcgcgcgcc tgcgccgtta
    17281 cttcgaacaa cgtgttgcca cacataaaga agctcactgg caggcttatt atcaagctcg
    17341 ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc atccggcatc
    17401 atagcccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg ccacgatacg
    17461 tattgattga aggcccggct gattttaatg accgggtaga cgaactgttt ttagcccacc
    17521 agcttccggt agctatttac agttattgcc agtatcagga cggtgcggcc cccgggcgtg
    17581 gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa gccgcacgtc
    17641 gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt gaagaagagg
    17701 acgattcgcc tgatacacaa gatgaaagcc agaccttact gctgcgtgcc acccgcatgg
    17761 ataacagcga taccctgtgg gatcacttat tcgaagatga aagccagcaa actgcattac
    17821 cctctacgct ggcgcactat tttgcccaac tgcggggcga ttcccccgga gatgctctca
    17881 atcgtcagcg cgaagccttt atggctcgct ggattacatg ggcgatgcag caaaacaatg
    17941 gcgacgtgtt agtcgtctgc ggcggctggc acgctcctgc actggcaaaa atgtggcgca
    18001 aatgcccgca ggaaatgaac aagccagaat tgtcctcgct ggcagatggc gttacaggtt
    18061 gttatctcac accctacagt gaaaagcgcc ttgatgtgct ggcaggatac ctttccggaa
    18121 tgcctgcccc ggtctggcaa aactggtgct ggcagtgtgg cttacagcag gcaggtgaac
    18181 aactgctgaa aacggttctt acccgtttgc gccagcacaa attgcccgct tctaccgctg
    18241 atatggctgc tgctcatctg catgcgatgg cactggcaca gttgcgcggc catacactac
    18301 ctctacgcac tgactggctg gatgccatag ctggctcgct gattaaagaa gccctgaatg
    18361 cgccgttgcc gtggagctat cgcggagtta ttcatcccga taccgatccg attttgctaa
    18421 cggtgataga cacattagcg ggtgacggat tcggtaaact ttccccttct acgccacaac
    18481 cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cggaatctct cttccggcgg
    18541 agcttacctt aaatcgcttt acccccgatg gactggcgca aagtcaggtg ttacatcggt
    18601 tggcaatact ggagatccca ggggttgtgc gccagcaggg aagtacgctg acacttgcag
    18661 gcaacggtga agaatgctgg aaattaaccc gcccgcttag ccagcatgcg gcattgattg
    18721 aggccgcctg ctttggtgcc acactccagg aagccgcacg ccataaatta gaagccgata
    18781 tgctggacgc gggtggaatc ggctgtatca ccacatgtct tagccaggca gcgttagcgg
    18841 gtctggcgtc tttcagtcag caattactgg aacaactcac attattaatt gcccaggaaa
    18901 atcaattcgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg cggctggatg
    18961 aaattagcgg tatgcaaggc gcgcagatat tacaaacgac attatgcgcg gccatcgatc
    19021 gcacgctgtg gctatgtgaa tccaacggca gaccggatga aaaggagttt cacgctcacc
    19081 tgcatagctg gcaggcgctt tgccatatcc tgcgcgatct acatagcggc gttaatttac
    19141 ccggcgtttc gctttctgcg gcggtagcct tactggagcg ccgcagtcag gcgattcatg
    19201 ccccggcgct ggatcgcggc gcgactcttg gcgcactgat gcgtctggaa catcccaacg
    19261 ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa tccggcgagg
    19321 cgctgcacgg tttgctggca ttagcccgtc atcaactggc ctgtcagccg gtatttatcg
    19381 ccggtttcag cagtcattta aatcaactga gtgatgccga ttttatcaat gccctgcccg
    19441 atttacgcgc ggcgatggcc tggctaccac cacgagaacg cgggacgctg gcgcatcagg
    19501 tgcttgagca ttaccagctc acacagcttc ccgtttcagc gctgcaaatg ctgttgcatt
    19561 gtccaccaca agccattgca catcatcaac aactcgaaca gcaggcactg gcatcgctgc
    19621 aacactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg tgagctacaa
    19681 cgctggcgat taattcttgg cgaagcggca gaaacgaccc tttgtgggct ggatgacaac
    19741 gcccggcaga tagaccacgc gctggagtgg ctgtatgggc gcgatcctga acggctccag
    19801 cgtggtgaac gttacggtgg attaggtcgt tcaaatctca ccacccctga gtggattaac
    19861 agcattcaca cgctgtttcc gcaacaggtg attgagcggc tggaaagcga tgccgtgctg
    19921 cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc tggaacgtat gcagccttct
    19981 gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaatcccga agtactggct
    20041 gccgcccgcc ggatagtgca ccaggttgtg gaagaaatta tggctcgact ggcaaaggaa
    20101 gttcgtcagg ctttttctgg agtacgcgat cgccgtcgcc gctcatttat ttcactggcg
    20161 cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca cccgcaacac
    20221 ggcaagttgt atatcgaatc cccccgcttt aacagccgca taaaacgcta tagcgaacaa
    20281 tggcaactgg tcctactggt tgatcaaagc ggatcgatgg ttgattcggt gatccactcc
    20341 gcagtgatgg cggcctgttt atggcagtta cccggcattc gtacccatct ggtcgctttt
    20401 gacaccagcg tggttgatct cacggcagac gttgccgatc cggtagagct attaatgaaa
    20461 gtgcaattag gcggcgggac caatatcgcc agtgctgtgg aatatggtcg gcaacttatt
    20521 gaacaaccag cgaaaagcgt cattatcctc gtgagcgatt tttacgaagg ggggtcatca
    20581 tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt gctgggactg
    20641 gcggcgctcg atagcagcgc aacaccttgc tatgaccacg atacggccca ggcgctggtt
    20701 aatgtcggcg cacaaatagc cgcaatgaca ccgggcgagc tggcttcctg gcttgcggag
    20761 aatcttcagt catgaattca ctacgtccgg aattattaga actgacgccg caggctctga
    20821 cggcgttaag caatgccggt tttgttaagc gcagccttaa ggaactggaa aatggcaacg
    20881 tcccggaaat cagtcatgag aacggcgctt taatcgccac cttcagtgac ggtgtccgta
    20941 cccagctggc gaacggccag gcactgaaag aggctcagtg cacctgcggg gccagcggca
    21001 tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgcc accgcccagc
    21061 ctacggaaaa aaaagaagaa gagtgggatc cagcaatctg gctgaaagaa ctggctaacc
    21121 tgcccgatgc cacccgcaaa cgcgcgcagg cgctggtcgc taaaggcatc accattgagt
    21181 tgttctgtgc gcctggcgaa attccctctg cccgattacc gatgagcgat gtgcgttttt
    21241 attcccgcag cagcattcgc ttcgcacgct gtgattgcat tgaaggcaca ctttgcgaac
    21301 atgtcgtact ggctgtgcag gccttcgtcg aggccaaaac tcagcaagcg gaatttactc
    21361 atttaatctg gcagatgcgc agtgaacacg tgacatcatc tgatgatccg tttgccagcg
    21421 aagaaggcaa aacgtgtcgt caatatgttc agcaattaag ccaggcatta tggctgggcg
    21481 ggataagtca accgccaatc cattacgagg ccgctttcag tcgcgcgcag caagcggcgg
    21541 aacgctgcaa ctggcgatgg gtgagtgaat cgctacggca attgcgcgcc agtgttgatg
    21601 ccttccacgc ccgtgccagc cactaccatg ccggagaatg cttacgtcag cttgcggcat
    21661 taaacagtcg attaaattgc gtacaagaga tggcgcgacg cgacagtatc ggtgaagttc
    21721 ctcccatgcc gtggcgcacg gtcgttggcg ctggcattgc cggagaagca aaacttgatc
    21781 atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcag tatggtttac
    21841 gcatctggtt taccgatccc gacacaggaa gtattttgca tctttcgcgt agttggcaga
    21901 gaagcgaaca ggaaaactca ccggcagcga cgcgtcggct gtttagtttt caggctggcg
    21961 cactggcagg tgggcaaatt gtttcacaag ctgcaaaacg cagtgccgat ggcgagctgc
    22021 tgttagcgac ccgtaaccgc ttgagtagtg ttgttccact gtcgccagac gcctggcaaa
    22081 tgttgagcgc gccgttgcgc cagccgggca ttgtggcttt gcgggaatat ttacgccagc
    22141 gtccccccgc ctgcatacga ccactcaacc aggtcgataa cttatttatt ctgccggtag
    22201 cggagtgtat ttcgctcggt tgggatagca gccgccagac actggatgcg caggtaatca
    22261 gcggcgaagg agaagataat gtgctgacgt tatcccttcc ggcatctgcc tgttctcctt
    22321 ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg agtctggttt
    22381 ctggctttgt cagttttgta gaggggcaat tgacactgga accacgggtg atgatgacaa
    22441 aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta ccttctgcca
    22501 gcgttctgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag gcgttactta
    22561 ttcaactgct ccataacggc tggcgctatc aggaacagag tgctattggt gaggcagagt
    22621 tgctggcgaa tgacctcacc gcggtgggtt tttatcggct ggcacatgtg ttgggacaat
    22681 ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt ttgctttgcg
    22741 aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttat taagagttct
    22801 actcctgttc cggactgctc acgccacagt attaggcata tcctatatag cccctggtga
    22861 gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg
    22921 ttttcccttg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa
    22981 gaatcaaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac
    23041 aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc ctccattggt
    23101 gcaaccacca aagaagatgc cgccaggaca cttgagccgt taagcgccaa atacaaaaac
    23161 atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga aaacgtgact
    23221 attgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caatgtttct
    23281 gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct
    23341 ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg ccatacccgt
    23401 cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt
    23461 gatcgccgca ttcgcggaag tttttatgat ccggtgcacg catcatcgcc gtaatttccg
    23521 gcatcgaaac gcggaactgc tgttcggtga ggattgccag aatgtcatcg gttttaagcg
    23581 aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca
    23641 gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgaag
    23701 acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg
    23761 cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc aggtcattat
    23821 tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt
    23881 caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt
    23941 aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg
    24001 gcctggccgt tatcttccag acggatctcc tgtaaatgcg cgaggttaac cagatactga
    24061 cgatggcagc gcagtaatgg tgtacgactt tccagggtac gtaatgtcag ttcggtaaag
    24121 ccctctttcc cttcgtgact ggtaacgtag acaccgctca tccgactgct gacaaatgcc
    24181 acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc
    24241 gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc
    24301 gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcatgt
    24361 tcttcaaagg ctttaatggc gtattcgtca aacgcggtga gaaaaacaat atacgggcga
    24421 tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg
    24481 agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa
    24541 cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt
    24601 gcaagcggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc
    24661 gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttatccca tagtcatcgc
    24721 caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta
    24781 ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc
    24841 gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt
    24901 tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa
    24961 tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt
    25021 tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc
    25081 ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca
    25141 ccgctttaat ggtgttaagc gcattaaaca aaaaatgggg attcacctgg gcgtgaagca
    25201 gtttgatctc tgactgggtg agcatcgctt tttgccgctc atattgcccg gcgaggatct
    25261 gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gttgattgaa ctgaataaac
    25321 ggtttttggc ttcatacaat ttgatggtac ccatcactcg ctgattttca ccacgcaacg
    25381 gaatgaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacgg taaggcactt
    25441 cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggta taagtcgaag
    25501 aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct
    25561 ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc tgtgccactt
    25621 tcatgctgtt cacttcgtta aacccctggc gcaaaatgcc ttccgtcgag gccgccactt
    25681 tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca
    25741 atatacgcat aaacaacgcc gcgccgacgg tattggtgac catcattggc gcagcaatat
    25801 tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg atcaacattt
    25861 gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag
    25921 tgcgcccgcg acggatcagg atgctgtgaa ccaggccgcc gagcaatcct tcaacgatgg
    25981 tcgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt aacccgccgg
    26041 tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta
    26101 tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc
    26161 agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca
    26221 ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca
    26281 tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc
    26341 tttaaaatgc gtaacataca ttgcttgcgt ttaactttct ttgaactctt gcagaaaaat
    26401 gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatagat
    26461 gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac
    26521 ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt
    26581 gatattaacc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa
    26641 cggacagacg gcggccatcg gctgttcaac gatgccgata ttgaccggat ccgcgagatc
    26701 aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtaa aaatgctgct cagtaatgaa
    26761 aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ttacctgcaa
    26821 agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc
    26881 cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg ccaacaaccg
    26941 actctccaga cgctgctggc gatcctcgac ggcgtactga tcaactacat ctctatttgc
    27001 ctggcttcgg cgcgcaaaaa acagggtaaa gatgcactgg tggttggctg gaatattcag
    27061 gataccactc gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat
    27121 gtcctcgccc actcgctcaa tcaactacgc cctgaactgt tcgaaggccg tacattgctg
    27181 gtgtggtgcg gtgataatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa
    27241 caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg
    27301 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
    27361 ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct
    27421 ttattttgcg tgctggctga acgcctgcac cagcctttca aacaggcggt ctgcgatgat
    27481 cgccgccagt gccaccagta acgctccctg gatcacatac gcggtattaa atccgctaag
    27541 tccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt
    27601 accaatgttg ataatcaccg aagttcgcac gccagccaga atcaccggag ccgccagcgg
    27661 tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg caacttctgt
    27721 cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg gcagcacgcc
    27781 gtaaaggatc aaggcgataa tcgctggttt cagaccaaag ccgatcaccg gaacggcaat
    27841 cgccagcacc gcgacgggcg gaaaagtctg cccaacggcg gcaatagttt ccaccagtgg
    27901 gcgaaattcc gcaccccacg ggcgagtgac agcaattccg gcaccagtac caatgatcac
    27961 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact
    28021 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
    28081 ataaggtagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca gcggatcgcg
    28141 caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc gtgccgcaag
    28201 gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagagaaa
    28261 gcgcatcgcg tagcgtcatc ctttcttcca gtgcctcacc ttcagcccgt tcttcacggc
    28321 gcacgtaatc tgccacgcta cgtaacgaaa gcaggcgtac acccagctca ctacgaccaa
    28381 aaaactggcg gacaaaatca ttcgccggat gagtcagcat cgccagagga ttgccctgct
    28441 gcacgacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa
    28501 tatcatgagt gaccagcaca atggtacgcc cctgcaaacg gtgaatgcgc gtcatctctt
    28561 gttgcaacgc gccgcgcgtt accgggtcca gcgcgccaaa aggttcatcc atcagtaaga
    28621 cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga ccaccggaaa
    28681 gctgatgcgg ataacgctca cgcaaatttg gctccagccc cagtagcgcc attaattcgt
    28741 cgatacgatc gtcgatccgc gcccgtgacc atttttgcaa ttgcggcacg gtggcgatgt
    28801 tttgtgccac gctccagtgg ggaaacaggc caatagattg aatggcatag cccatccggc
    28861 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atcaccccgc
    28921 tgtcatgctc caccaggcgg ttaatcattt tcagagtggt ggatttaccg gagcctgatg
    28981 taccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt
    29041 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
    29101 agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat taccgggatc
    29161 acccccagca acactaaatc aatggcactg cttagcagcc cctggaaaac cagcgcacca
    29221 aaaccgcctg cgccgattaa cgccgcaatc accgccatgc caacggtttg caccatcacc
    29281 acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaaacgc
    29341 tgcaccccgc tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
    29401 aagccgacta ctacgccgcg caccagcggc agcaaggcat agagcaccag cgcaataagt
    29461 gcgggtgtca ttccggttcc tgctatgccg agcgttccca gccacggaaa ggccgtaacc
    29521 agcgcggcaa gcggcgcaat cagcaggcca aagagcgcta ccgaaggcac ggtctgaatg
    29581 acattcagca gagaaaaaat cgccccctgc cgcgcggtgg aaaagtagca ccagatgccc
    29641 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
    29701 gccagagcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattaa cgagagatcg
    29761 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
    29821 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcagtgcc
    29881 agcgccgcag ccagccagaa accactgccg aggctggtac gtgccagcgc actgccattt
    29941 tgcgccagtt gtgtcgccgc ctttccagct ccccacacca gcaatacgaa gacgaattgc
    30001 gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc tgtcagccag
    30061 gcgcaaccaa cgcccaccag catccagaga gtttgcggcc acagctgcca gagatgacgc
    30121 ccctcacccg aaactaaacg attaggcgcg taactgataa acggcagcgc cgctgcgatt
    30181 gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt cacgggaaat
    30241 tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct
    30301 tccacagcaa tgctggcatt cagttgctgc aatgtttttt catcgaggct ggcgaagact
    30361 ggctgtagcc actgtgccat ttgtggatac tctttcagca ccgactcacg caccactggt
    30421 gcaggcgcgt agataggttg cacgccttgc ggatcgctta aggtttgcag ccccagcgcc
    30481 gcgaccgggc cgtcagtgcc gtaagccatt gcggcattaa cgccagaggt ttgctgggca
    30541 gcggctttga tcgttaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg
    30601 agcttaaagc cataggcttt ttcaaacgcg ggtaaggcat cggcgcgttc aataaactct
    30661 gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat aacgactcag gtcggcaagc
    30721 gaagtgagct tgtttttctc tgccacatcc tgacgcacgg cgatggtcca ggtgttattt
    30781 gcaggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt tttgactttc
    30841 tcgtaacctt gctgcacgtt tttccacgct gcatcgtttt catctttaaa gaagaacgcc
    30901 ccattgccgg tatattccgg atagatatcc agctcgccag aagtgatcgc gccgcgcacc
    30961 acaggagtcg ttccaagttg cactttattt accgtaggaa cgccgtgact ttccagcacc
    31021 tgcaaaatga tattgccgag cagcgcgcct tccgtatcga tttttgaacc aaccttaacg
    31081 ggggaagctg cctgtagcgg caggctcacg gctgccaaca aaaccagtga acctgcccag
    31141 acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag
    31201 agaaaagcat agttgataat gacagggtta gcctgtctgg gtggattcag ttgcagaatc
    31261 agataaatac ttaaaaggca tattttgccc ggcgtttctt gccggatgcg gcgtaaacgc
    31321 cttatccggc ctacaaacat cgaaaattac aacaactcaa actcggcttt cttaacgcgt
    31381 gcggaatcag tgccgataaa gacattgaac ttgccaggct cggcgtcata tttcatctgt
    31441 tgattccaga acttcagcgc ctcaatatca atcgggaagc tgacggtctg agtttcgccc
    31501 ggtttcagag tgattttctc aaagcctttc agctgcttca ccgggcgact catggaagcc
    31561 gtcacatcct gcaagtacat ctgtactacc gtggcccctt cgcgtttacc ggtgttcgtc
    31621 acctgcacgc tggccgtcac tttgccgtca cgcttcatgg tcggcgcaga aagtttcaca
    31681 tcagagacgg tgaaagtggt gtagctcagc ccatagccga acggataaag cgccccgtta
    31741 gcttcatcaa aataacgcga agtgtatttg ttcggcttgt cggcattata cggacgcccg
    31801 gtattcagat ggctgtagta caccgggatc tgcccgacag aacgcgggaa ggacatcggc
    31861 agcttgccgg acgggttata atcgccaaac aatacatcgg caattgcatt accgccttca
    31921 gtccccgcaa accaggtttc cagaatcgca tcagcctgct gatcttcttt caccagcgcc
    31981 agcggacgcc cattcatcag caccagcacc agcggtttac cggtggcttt cagcgccgca
    32041 atcaagtcac gttggctttg cggaatagtg atatcggtac gactggaagc ttcgtgcgcc
    32101 atcccctgag cttcaccgac cacagccacc accacatcag attgtttcgc ggtttgcacc
    32161 gcttcatcaa tcatctcttg cggcgaacgc ggatcgactt tgaccgcttc ttcatactga
    32221 ttcaggaaat cgataatgcc tttgtcactg gtaacgttcg cccctttggc atacagcact
    32281 ttaccgtttt caccgacggc gtttttaatc ccggttagca cagtaacaga ttgatcggcg
    32341 acacctgctg ccgaccagct tcccatcacg tcacgcttgc tgtctgccag cgggccaacc
    32401 accgcaatgg ttgcagattt tttcagcggc agcgtttcga gacggttttt cagcaacacc
    32461 aggctttcgc gcgccacttc acgcgcttct ttgcggtgca ggcggctttc ggcattggta
    32521 tccaccgggt cagactcttt cggccccaaa tggctgtatg gatcgttaaa caatcccata
    32581 tcatatttaa cgttcagtac atggcgggca gcatcgtcca gctcttccat cgtcactttg
    32641 ccggatttga tcaacccagg cagatacttc gagtaatact cgtcgctcat gctcatgttg
    32701 attccggact tcagcgccac gcgtaccgca tcctctgggt ctgccgccgt gccatgttta
    32761 atcagttctt tgattgcgcc gtgatcggaa acggtgatgc ctttaaagcc ccactggtcg
    32821 cgcagaacat ctttcagcag ccaggagtcg gaggtggctg gcgtgccgtt cagcgagttc
    32881 agcgccacca tcaccgcacc gctgcctgca tccagccccg ctttgtacgg cggcatatag
    32941 tcgttaaaca ggcgctgcgg actcatatcg acggtgttgt actctttacc gccttctacc
    33001 gcgccgtatg cggcaaagtg tttgacgctg gtcatcaccg agtagcgatc tgccgggctt
    33061 ttaccctgca tcgcttccac catggtttta cccattattg aggtgagata cgtatcttcg
    33121 ccaaaacctt cggaagcacg tccccagcgc ggatcgcgcg agacatcgac catcggtgcc
    33181 caggtcatat tcaggccatc atctgccgct tcataagcag agacacgccc gactgttttc
    33241 accgcatcga ggttaaaaga cgaggccaga ccgagactaa tcgggaacac cgtgcgctga
    33301 ccgtgcagca cgtcgtaagc aaagaaaaga ggaattttca ggcggcttaa ttccatcacc
    33361 tgatcctgca tggcgcggat atcctgacgg gttacggtgt tgaaaatcgc cccaacctga
    33421 ccgtctttga tcatctcgcg gatcgcctct ttcgggttat ccgggccgac gctgattaag
    33481 cgcagctgac caattttctc atcaactgtc attttcttaa gcagttcggt gacgaacgca
    33541 tcccgcgctt cgggcgttaa tggatggttg ccgaataaat catccgccag tgctggctgc
    33601 agggccagac tcaccgcgat tcctactgaa catagccatt tcatatggat ttttcttcct
    33661 ttgttgcccg acgtggcagc gaaaatggtg caaaaaccgt agtttgccat aagcatgatg
    33721 gagagagaaa aagaatgctc agtttattgt ctgaattttc aaaatattca ctcgctgaat
    33781 tgtcatacaa ggcgctattc tagtttgtga tattttttcg ccaccacaag gagtggaaaa
    33841 tgtcttccat gacaacaact gataataaag cctttttgaa tgaactttcc cgtctggtcg
    33901 gtcattcaca cctgctcacc gatcccgcaa aaacggcccg ctatcgcaag ggcttccgtt
    33961 ctggtcaggg cgacgcgctg gctgtcgttt tccctggctc actactggaa ttgtggcggg
    34021 tgctgaaagc ctgcgtcacc gctgacaaaa ttattctgat gcaggcggcc aatacaggcc
    34081 tgaccgaagg atcgacgcca aacggtaacg attatgatcg cgatatcgtg atcatcagca
    34141 ccctgcgtct cgacaagctg cacgtactcg gtaagggcga acaggtgctg gcctatccgg
    34201 gcaccacgct ctattcactg gaaaaagccc tcaaaccgct gggacgcgaa ccgcactcag
    34261 tgattggatc atcgtgtata ggcgcatcgg tcatcggcgg tatttgtaac aactccggcg
    34321 gctcgctggt gcaacgtggc ccggcgtata ccgaaatgtc gttattcgcg cgtataaatg
    34381 aagatggcaa actgacgctg gtgaaccatc tggggattga tctgggcgaa acgccggagc
    34441 agatccttag caagctggat gacgatcgca tcaaagatga cgatgtgcgc cacgatggtc
    34501 gtcacgccca cgattatgac tatgtccacc gcgttcgtga tattgaagcc gacacgcccg
    34561 cacgttataa cgctgatccg gatcggttat ttgaatcttc tggttgcgca gggaagctgg
    34621 ccgtctttgc agtacgtctt gataccttcg aagcggaaaa aaatcagcag gtgttttata
    34681 tcggcaccaa ccagccggaa gtgctgaccg aaatccgccg tcatattctg gctaatttcg
    34741 aaaatctgcc ggttgccggg gaatatatgc accgggatat ctacgatatt gcggaaaaat
    34801 acggcaaaga caccttcctg atgattgata agttaggcac cgacaagatg ccgttcttct
    34861 ttaatctcaa gggacgcacc gatgcgatgc tggagaaagt gaaattcttc cgtccgcatt
    34921 ttaccgaccg tgcaatgcaa aaattcggtc acctgttccc cagccattta ccgccgcgca
    34981 tgaaaaactg gcgcgataaa tacgagcatc atctgctgtt aaaaatggcg ggcgatggcg
    35041 tcggtgaagc caaatcgtgg ctggtggatt atttcaaaca ggccgagggc gatttctttg
    35101 tctgtacgcc ggaggaaggc agcaaagcgt ttttacaccg tttcgccgct gcgggcgcag
    35161 caattcgtta tcaggctgtg cattccgatg aagtcgaaga cattctggcg ctggatatcg
    35221 ctctgcggcg taacgacacc gaatggtatg agcatttacc gccggagatc gacagccagc
    35281 tggtgcacaa gctctattac ggccatttta tgtgctatgt cttccatcag gattacatcg
    35341 tgaaaaaagg cgtggatgtg catgcgttga aagaacagat gctggaactg cttcagcagc
    35401 gtggcgcgca ataccctgcc gaacataacg tcggtcattt gtataaagca ccggagacgt
    35461 tgcagaagtt ttatcgcgag aacgatccga ccaacagtat gaatccgggg atcggtaaaa
    35521 ccagtaagcg gaaaaactgg caggaagtgg agtaataatt acggatggca gagtatcgcc
    35581 atccgtcatt catttaatcg ttctgtgccg tctgccccgc cgccgccatt tgggcggctt
    35641 tttgtttttt atagctcaac gctgctgccg gaacaggcat cactttaccg gtttcaatcc
    35701 aggtacgcag gcggctggca tcggcaaaat gggtatattt gccaaacgcg tccatcacta
    35761 ccagcgccac cggtttatta ttgataaccg tgcgcatcac cagacaatgg cccgccgcat
    35821 tggtaaaacc ggttttggtt aactgaatat tccagttatc acgatacacc agatggttag
    35881 tattgcggaa cggcagcgta tacgtcgggt tagagaaggt tgccatatct tcccgggtag
    35941 tacttaactg cccgatcaac ggatattgtt tgctggcaat gagtagtttg gttaagtcac
    36001 gggcggttga aacgttatgc accgacaatc cggttggttc aacaaagcgc gtgttgttca
    36061 ttccgagcga tttcgctttc gcattcatcg ccttaataaa ggctttgtaa ccaccgggat
    36121 aatggtgcgc aaggcttgcc gccgcgcggt tttctgaaga catcagcgcc agcaacagca
    36181 tatctttacg gctgatttcg ctgttcagtc gtacgcgcga atagacccct ttcatctccg
    36241 gcgtctggct gatatccact tttagttttt catccagcgg cagtcgtgca tccagcacaa
    36301 ccatcgcggt cattaatttg ctgatagacg caatcggacg caccagatcc gggtggttcg
    36361 aatagatcac tttgttggta ttcagatcaa caatcatcgc gctaccggag gcaatttccg
    36421 gttgtgacgc ggtggtagcg gctgccgttt tcgcaacggc ctgcggtgca aaaggcacag
    36481 ccagcatcag ggccaggcta aataaagaaa ctcgaaattt cggcatgatg agcattcaga
    36541 tagtggttca cgcgcacggg ttgcgcaccg ccggagtaag gatttactga ggctagcgac
    36601 gccatcataa cgagcaaaaa gtgcgatcgt caaaggagaa tcgtgaggaa atgctgcatt
    36661 gctgacattt acgccagcaa tgcagcgcaa aagaactttc tagaacaaac gataaccgta
    36721 gccccatagt ataacggtaa gggcgagcag cacttccagc accagcacgc ctatcgccag
    36781 tgtcgaactg gagaagctaa gtccttcctc tttgttgata ttcaggaagc tcggaatacc
    36841 gagatacagc aggtagccgg tgtaaaacag cgccaccgtg ccgaccagcg cacacaacca
    36901 gaccagcggg taaagcgcca ccagaccgct taaaaacagc ggagttgcaa cgtagcccgc
    36961 gaagaccatg cagtgcgcaa gtgacggacg ctgcggataa ttacgcgcca tccaccagat
    37021 gacccgcccc atcaccgcaa ccccagccag cataacgccg taaaacaaga cagccagcgc
    37081 cagtccggta aaccaggata acttcaggat agtgccatcg ccaaaattcc agccaatctg
    37141 tgtagtgcca atgaaggcgc aaatcaccgg aatcgccgcc atcagcaaaa cgtggtgggt
    37201 gtagtgatga gaaatcgttt cgttttcgcg attaatcacc tgcatttcac gatcgggatg
    37261 ggaaaacagt ccccagacat ggctcatacc gcccccttgt tgtgagttca tgaacctgac
    37321 agttcaagta taagtcagct tgtgattatt ttttgttcac tcatgtaatt tgcaccgtct
    37381 gatgtacgtc ctttttcagc gtgaatgatt cacagggtgt atgcttacag gcaagcaaag
    37441 ggagacagac tgtcctatgg atctcaatac actcatctca caatatggtt atgccgcgct
    37501 ggtgatcggt agcctggcgg aaggtgaaac cgtgactttg ctgggaggcg ttgcggcaca
    37561 tcaggggcta ttaaagttcc cgctggtggt actttctgtg gcgcttggcg gcatgattgg
    37621 cgaccaggtg ctttatctgt gcgggcggcg gtttggcggc aagctattac gccgtttctc
    37681 gaaacatcag gataaaattg agcgggcgca gaaactgatc caacgccatc cgtatctgtt
    37741 tgtcattggt acgcgtttta tgtatggctt tcgggtcatt ggcccgacgc tgattggtgc
    37801 cagccagctg ccgccgaaaa tctttctgcc actgaatatc ctcggcgcat ttgcctgggc
    37861 gttgattttt accactatcg gttacgctgg tggtcaggtg attgcgccgt ggttgcataa
    37921 tctcgaccag catttgaagc actgggtctg gttgattctg gtcgtggttc tggtggtagg
    37981 cgtgcgctgg tggctgaaac gacgcgggaa gaaaaagccg gataatcagg cgtaaaacca
    38041 ttgcctagga taaggcgttc gcgccgcatc cgacatccgg tgcaccatgc ctgatgcgac
    38101 gcttgtgcgt cttatcatgc ctacccaaat attacccatt gaaataacca cgcacctcac
    38161 tgcgctattc tgggttgaac tgcggattcg ccagcataaa gccgccatcc actatcaacg
    38221 actgcccagt ggtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct
    38281 catgcgttgc gccaaaacgc cgcaagggaa tcgaaggctc cgcgccgggc ttcacgtcgc
    38341 catcatccat gccgttcatt ggcgtggcga tcgccccagg tgcaaccgca ttcaccaaaa
    38401 tcttatgcct gaccagctcc agcgccatcg ctttggttaa cccgccgagc gcatgtttag
    38461 cggctgtgta ggcgctggca tccggcaacg gcgtatgttc atgcaccgac gtaatgttga
    38521 tgatgcgacc accctgccct tgtttcacca tctggcgggc agcaatttgc gagcataaaa
    38581 atgcaccatc gacatcaacg gtaaaaatct tgcgccactc atcgaaagcc atatcaagaa
    38641 acggcgcttt ggtcattgcg cccgcattat tcaccagcac atcaatacgc cccagccgtt
    38701 gaatgagttt ctccagcgcc agtgcccctt ctggtagatt gccgagatcc agctgcacga
    38761 tctccgcacg tacgccgtgg ctaactacct cacgcgcggt atcttttgcc ccttcttcat
    38821 ctgagtgcca ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt
    38881 tgccgatccc cgaatcggag gcggtaataa tcgcgacctg tgccatcgag ttctccactt
    38941 aacgctgaat aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc
    39001 aacgcaccag tgagttgaat ggaggcatcc agccactgcc cttgcaataa caggccattg
    39061 gcccgctcac gcagcgcggg gattctggct tcgctgacgc gggaacccgc aatgatgccc
    39121 gcgttaaagc gcgcctgcgc aagacctact acacgcaagg catcgcgctc aatttgcgcc
    39181 tgatgctggt ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc
    39241 accgcttcaa ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg
    39301 atatcgaggt tggcgttaag acgaccacta tcgaaaatgg gtagcgtcag gcctgccgta
    39361 acgcccattt gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc
    39421 aggaaggcca tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt
    39481 aacgatgact caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag
    39541 taccccagtt catcaggaag ctgacttgcc actttcggca acgcgaccgg attaagcttc
    39601 aatgcctttg tctggtgatt tgtaagtgcg cttaaccgtg cctcaataat tttcattttc
    39661 cccgcgacat cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacacct
    39721 tcaactgaag aagtaatccc gttctgatat agctggcgat cggtggcgat aatggtgttc
    39781 tgctcttttt cgatttgctg taagacagtg tttaacgccg cctgggtttg ccactcccag
    39841 tacaggcggg ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgctcgt
    39901 gctttaaccg tacccaggcg agcagttacc tctgcccgat tctttcccca gatatcgaga
    39961 tgccagcccg ccgttaagcc aaaagtaccg ttggtgtacc acggaccggt cgtacctgcg
    40021 gcaggatcgt tcagagcaaa cggccccatt aacccttctg cagacatttt ttgccgctcc
    40081 atatccgcag aaaagtcgat ctgcggacca tcctgagtag caactgcctt cgcctgggct
    40141 tcagccagtt gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg
    40201 ttaattaagg aagtgagttg attatcgtga tactccagcc accattggct gtctggccaa
    40261 ccatttttca gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt
    40321 aacgcctggc gggtttcatg cataggtgca cacccggcca gcatcagtaa cagcggaaaa
    40381 caggcgatgg cgggataaaa ggaatcacga ttcatggggg aataatcagg taagaaaagg
    40441 tgcgcggaga ttaccgtgtg ttgcgatata ttttt
//
LOCUS       DACADA010000025        39117 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.025, whole genome shotgun sequence.
ACCESSION   DACADA010000025 DACADA010000000
VERSION     DACADA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.025"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            72..1100
                     /locus_tag="ISB17_001291"
     CDS             72..1100
                     /locus_tag="ISB17_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1979243.1"
                     /translation="MSVLYRWLEDRQIDLRARLKIGTLFTEAEFSGGLIEFLRKDMES
                     EDKVVSPHTFNNRLATIRQYFVWELDVYISSLPLSDSQYEVLQAARKRLMGWIDRAFI
                     NTPRAGSIPRKSLTNDEAEFLLQCLNPESPDSFAHFEPVKFRNFAAVSLMLNCGLRPG
                     ELLSLRVEDIQIGSISSVTVKRRSPDPLDKRKPRPSVKRNGRVLPLEGHHFVRMLDRY
                     IVEWREILEQKSSSETDYLILSDEGDPLSHSSLTQLFSRLRSEYSGLLPEILTPKSLR
                     HTFSSRMEQVLRAAGMEEDRRKQALAMLRGDSSLESQSVYIAQEVEEQARRALSDYQK
                     KLITGFNK"
     gene            1097..2695
                     /locus_tag="ISB17_001292"
     CDS             1097..2695
                     /locus_tag="ISB17_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1979244.1"
                     /translation="MNLLLHKAPQISDSALELPSQFFTREGVKVEITGDRWTFPLLYR
                     HSRLDFSRVENDTLKTSIKRCIIDQASRVSTHAGCQYWSDISAAIVSRQKKHSLLPDI
                     SADEFQERLIAVMEEAISQARNKHRLWALYRPIQWYIWCSENYSELGFSIAYAQELDS
                     LEIPGNPKGEAVRQEDPECGPLHRTLELQPIINALKQDQSKVYEHLQQKTAVALSIAF
                     GRNPANLTFLKETDLVDLTPGSSPPCYVLKMPRIKKRQLAPRDDMIDEYLEPEIAKHV
                     LELIDASRHRKLLIEVDGKAVEVTRPLFINPRKNAYAIDSKRWDEAYNMLSTGIANLV
                     KAFIKRHNIISPLTGELLHVTPRRLRYTLATGLAAEGISKRELARILDHTDTQHVNVY
                     FEMAGKIVEHLDKATAKGFSKYLNFFKGRLIDSDEDAVKGERDDKHLTFVDDQNPADQ
                     ADIGVCGESSVCHLNPPYSCYLCPKFQPYRHADHEHVLECLLAGREERLKKYENARLG
                     MQLDEVIAAVAQVAKLCEEGGHHV"
     gene            2688..4697
                     /locus_tag="ISB17_001293"
     CDS             2688..4697
                     /locus_tag="ISB17_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP1979245.1"
                     /translation="MSDGRSNRKAKVVPFVDRLERDRNENLNSLVSKAKLMKLEGFES
                     VVWEDPEWQVDAGRLVKLTGKNTKSASFGFLLSPKLGSAPLDGCWELVAKALFVLRFH
                     RKYQSAPNQRSFITAIGYVSFAAKQLGQELVGLTPEALDNASGLISKHYSETTAYNLH
                     KHVAEFAAHCDSNGLCRVLFQYKYAKMKRPASTGGINHKRLDDPEALDTKSDKLVDPA
                     VFRVIGELYLNVPKEHKYRFYILMLTLLACTGRRFSEISLLPNQQLSSDEEGIAYIEY
                     FPRKASRGDVFTPKRRLYLPSEVTPIVGDVLVELAEITFEARLTAEEIHKVGEPDLRF
                     LDGIPEDKKLYAADLTELGISHTVLGTTGWLRKQNLAWPDHSALTKQGIKPANPIFYT
                     YKSGLVEYCFRDFSGAYLSALHTDQFGKEYYLKDLLFIRSLGLSSGSYAHWLATSCSQ
                     SMFTTFLRYFPALAAEYASSSIEVDFTSHHFRHTLNTLLDEGGLSDLLQTEWFGRTNP
                     RDTKAYQHTSREKRALMLREDIKKGLVGGQLAEQIKVVPVEVQDAILKARIQAVHDVG
                     TGICIHNFSQTPCERHLQCSADCKDYVWAKDDKGRLDEQKRQYALTALARKKAEQQLD
                     STKPKKSADWLAHNDKKLKTLAAQLADNGVEHFDPEQYLHEVEHG"
     gene            4690..5166
                     /locus_tag="ISB17_001294"
     CDS             4690..5166
                     /locus_tag="ISB17_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAP1979246.1"
                     /translation="MVKRFRRMSDSDINTIVADLDRWALGELGSKLTWAVLEERFGFS
                     RQSLQAKSEIKAAYDNAKRALSGGLVKTKEQATKEAEELQVEVDRLKAELDAYKRKEE
                     QWLRRWQQIAFHVRQKGIQMASVDKAPPEGAELPSNTETAQILRPFDKEVPPSGRV"
     gene            5227..6369
                     /locus_tag="ISB17_001295"
     CDS             5227..6369
                     /locus_tag="ISB17_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979247.1"
                     /translation="MQKRLNTDKAQQAIDKAGLTQTAVADALGVTKEAVSQWLLHKSF
                     PRPNKLLQLGKLLNLSFDDLVIKEDPHAPKVAFRKMRGTKTKDYHIEKAQEIGRFLRH
                     LVPYLPFDTFEMPPVLKSPSCDYDYLRTVTAKVREDINLGPVECVDFTHLIRRFSELQ
                     AVVVPVMWGAKQRHENAVHIHLPDSGSTWVYLNLDTNTHDFKFWMAHELGHCLSPSLE
                     GDDAEDFADAFAASLLYPHELAEQAYEVLVKKPSTAAKINTLKQMAELQVISPNTILQ
                     QVNKYAKRLDRAEIIMGNSFFGAVTKFNQQFLNISEALFGDNELDEYGKPSARDYIEK
                     VEGAFETPFFDMLRQYLKEHDKGPGYVQTVLDMPLLDAQSIHAELT"
     gene            6369..6920
                     /locus_tag="ISB17_001296"
     CDS             6369..6920
                     /locus_tag="ISB17_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1979248.1"
                     /translation="MPQSKILVDTNAYLRLAKTVRPLLFVPFGDNEYCLYILPELNEE
                     LAGRKLQSKFPWVDDEEFAENRKHFPQIGKKQKKSIQQTFEYVWDHVQTELPGPSKVD
                     TWYVAYALELGVPVVTDDQDMTDLAKAFDAQVMPTLELLKIMLDCGHTDMKTINGLVE
                     YWKYFADMPANFKPDYQRLFGDQ"
     gene            7018..7242
                     /locus_tag="ISB17_001297"
     CDS             7018..7242
                     /locus_tag="ISB17_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979249.1"
                     /translation="MSDPILTIKDVADYLKVNERTIYRLAASSELPGFKVGNSWRFKQ
                     SELEQYIAAQHNRAGVSEAMKSPNSKSEDN"
     gene            7255..9396
                     /locus_tag="ISB17_001298"
     CDS             7255..9396
                     /locus_tag="ISB17_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAP1979250.1"
                     /translation="MAQLENIEAIEKRLWKSADTLRANSELASNEYFLPVMGLIFLRH
                     AYSRYLSLKDEIIANLPSRGGKTRELTKEDFSKKSAIYLKPEAQFDYLIALTDADNRA
                     EAIIHAMDSIETDYTNLRNQLPKQEYNNIPNDVLGMLLRTLNPEELKKATGDIFGRIY
                     EYFLTQFADQGAHDGGEFFTPVSLVQLIVNVLEPDHGKIFDPACGSGGMFVQSAHFME
                     RHAQDPHELTFYGHEKNRVTTRLAKMNLAVHGLEGNVEGGEAAITYYNDPHEGLFGTV
                     DYVMANPPFNVDEVDADKIKGDKRRLPFGLPGVNKNKKVSNANYLWIQYFYSYLNDSG
                     RAGFVMSSQASSAGRDEAKVREQLVKTGHVDIMVDIRGNFFYTRTVPCQLWFLNKNKP
                     AHLKDKVLMLDARNVYRKVTRKIYDFSPEQQQNLTAVVWLYRGEGERFVELVQQYIDK
                     SIFEARSCEQSEALACEPIPDFITQLGKFGQAFQPFMDKLEQGGVSVEPYVDFLNAKG
                     SVEGGWSAFQILTNDLQKWWNEYDYNDAASLLDFIDKDTCLKDLAEQSRNLIKEIDLA
                     YKLATRVIELAEANDAKDSELWDNTLLNGRSRSNLKKTADDARKLAVEQLKQVRYFYK
                     QAHWLLSRFPEGKLCDVEGLVKLVDIKAIEAADWSLTPGRYVGVVPEEVDEDFDFEET
                     LHDIHIELQGLNSEAIELAEKIARNFEELGV"
     gene            9393..10547
                     /locus_tag="ISB17_001299"
     CDS             9393..10547
                     /locus_tag="ISB17_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP1979251.1"
                     /translation="MSWQSEPLSALTLKIGSGSTPRGGDSVYISEGTTLIRSQNVYNN
                     EFSTDGLVYIDEKTAEKMKGVSVEKEDVLVNITGDSVARCCLVHDAVLPARVNQHVAI
                     LRTNPKKLLPHFLAFYMVSPFMQAKMLSWAGTGGTRKALTKGMLEGFEIPLPPVDVQD
                     KIVQKIKTYNDLIENNRRRIQLLEESARLLYQEWFVHLRFPGHKQVKITDGVPEGWSK
                     EPLENLLVLQRGFDLPVSKRIEGSVPIYASTGINGFHNVAKVKGPGVVTGRSGSLGTV
                     MYVAKDYWPLNTTLWVKEFKKASPIFATYLLRAMKLEGYNGGAAVPTLNRNDVHKVDV
                     LCPESKLMNEFEVQVENIFKQIDKLKEYNEKLAQARDLLLPKLMSGELTV"
     gene            10562..12694
                     /locus_tag="ISB17_001300"
     CDS             10562..12694
                     /locus_tag="ISB17_001300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP1979252.1"
                     /translation="MNRAQAQQLLQTALANPAAEFRDGQWEAIDALVNHRQKLLVVQR
                     TGWGKSSVYFISTKIFRDRGMGPTIIVSPLLALMRNQIESAARLGIVAETMNSTNTDD
                     WRSVTQRILNNQIDCLLISPERLANDSFIETVLQPIADRIALMVIDEAHCISDWGHDF
                     RPDYRRIVNILRQLPANTPVLGTTATANNRVVADIQTQLGNIQIHRGPLIRESLSLQT
                     MVLPDQASRLAWLAQVIPTLPGTGIVYVLTQRDADLVAKWLNQQGISALAYYSGVTHP
                     NFVNYAGKEDTDLYRQFLETQLLENKIKVLVATTALGMGYDKPDLGFVLHYQAPSSIV
                     AYYQQVGRAGRGIENAIGVLMSGTEDNDIHAFFRRTAFPSQDDVQRLLAALEDADGLS
                     LEKLEKEANLPQKRIEHVLKYLRTESPSPVIAIGKTWFRAPVRYQMDLEHIRKITEIR
                     EREWSEINDYLNTSGCLMLALRNALDDPSQETCGKCENCIAEPVLTKVFDQEIGIRAT
                     QFLRHSEEIIKPRKQIAASNDEAARTFVEYEFPRNLGDLVAQEGRVLSRWGDAGWGRT
                     VADNKHAGRFSDELVEAMAEMIQQRWQPNPAPQWVCCVPSRNHPELVPDFARRLAARL
                     GLPFVDAVSKVKDNQPQKGQQNRFHQCRNLDGAFAVTQLYQGQPVLLVDDIVDSGWTL
                     TVVAALLQQAGSGVVYPIALASSSVKDS"
     gene            12691..14079
                     /locus_tag="ISB17_001301"
     CDS             12691..14079
                     /locus_tag="ISB17_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP1979253.1"
                     /translation="MNVSRFSSADGSLPANGSLSPTAQATLLLTSYFSKASSESAKPL
                     TNAEWGRFALWLKEKSTTPADLLVSDPKALLQGWHDARISAERIIELLSRGHSLALAM
                     EKWQRAGLWVVTRSDPEYPRRLKQQLKTDSPPVLFGCGNKALLSAGGLAVIGSRNASE
                     SDLAFTDQVGAKAAAEGIAIVSGGARGVDETAMLGAMKQGGVVIGVMADSLLKAATSS
                     KWRKGLMNGHAVLVSPFYPEAGFSAGNAMARNKYIYCLADSSLVIHSGKKGGTLNGAE
                     ENLKKGWVPLWVKHTTDKDAANADLVTKGGRWLEADNTQAFKIAELMCNEGGRVNQVK
                     EQMGDLFSMPAQPELFFEPESNPEPDSGPEEVIESEREIVVSESQTKIQAIPEQTAES
                     EAAPSGPVDFYQMFISELQHLAEKPVTVDALIESTQLHKSQLTEWLKRAVDDGVVKKL
                     NRPARFQVKNKK"
     gene            14092..17334
                     /locus_tag="ISB17_001302"
     CDS             14092..17334
                     /locus_tag="ISB17_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1979254.1"
                     /translation="MAMTEETLVQEVTADYLLNQLQWDESVLGMYEKLGKEGDLGRLS
                     EQEVVLTRYLGEKLIELNPGLPDVAYQEALRVVCEMPASTNIVAVNKEKYSLHKNGVE
                     VSFHNEKGERVKKRLRLFDFDNYENNHFLLVREFWIKGDIYRRRADLVGFVNGIPLLF
                     MEVKNVHKDIRAAYEQNLADYKDTVPHLFYHNAFIILGNGIDAKIGSVSSKFEHFNDW
                     KRLNESEPGVVDMETLLKGTCSKANLMNIFENFTLFDQSSGRLVKIIARNHQYLGVSR
                     AIDAVINRGERMGKLGVFWHTQGSGKSYSIVFFAQMVHRKLGGNYTFVVLTDREDLDT
                     QIYKTFAGCGLVDNDKDPCRADSGKDLKELIGQQKAFVFTLIQKFNEKVDPKKPYSER
                     DDIIVITDEAHRTQYGTLSLNMRNALPNASFIGFTGTPLFKDDEITKKVFGDYISTYD
                     FQRAVEDKATVPLYYDARGEELVFTDEDGNEHTVADPKGINERIAEKLDELEIEDVDV
                     QQRLERELKRDYHIITATSRLDQIARDFVAHYANAWETGKAMFVCLDKITCVRMHKLI
                     DFYWQQEIKTKEKALLSATDEQDLAWRERQLAWMKETLMAVVVSEEQGEVAKFEKWGL
                     DIKPHRQLLKAGLELADGSRLDMEEAFKSSEHPFRVAVVCAMWLTGFDVPTLSTLYLD
                     KPLKAHTLMQAIARANRVAEGKNNGLIVDYCGILKNLRKALATFAGAGDEGHGGGEGE
                     NEPAKPNEELLESLNESISFVAEFLRKHDFELSRIITETGFAKNAAIAQAKEIINQND
                     ETRKRFEVMAREVFNKFKACINVPGVNGYRDMRDAINVLYKSLQADKEKADISDIMKD
                     LYEIVDGSIDTTHKVKEPKPDGDRIYDISKIDFERLRQEFSRSERKNTTVQSLKHAVE
                     KKLARLMMQNPLRTDYQEHYEKLVKEYNQEKDRVVIEKTFEALLKLNEELSHEEKRAI
                     RECLDEESLVLFDLLSKPNLQPKEIAKIKQVATTLLATLKAERLKVANWQQKESTRDA
                     VKQQIFDFLYDERTGLPVEQYEENEISDITERVFLHIYRAYPELPSPIYG"
     gene            17735..18855
                     /locus_tag="ISB17_001303"
     CDS             join(17735..17993,17993..18855)
                     /locus_tag="ISB17_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HAP1979255.1"
                     /translation="MKKRFSDEQIISILREAEAGVPAGELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     ATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
     gene            complement(19165..19446)
                     /locus_tag="ISB17_001304"
     CDS             complement(19165..19446)
                     /locus_tag="ISB17_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979256.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            19875..21461
                     /gene="pdeC"
                     /locus_tag="ISB17_001305"
     CDS             19875..21461
                     /gene="pdeC"
                     /locus_tag="ISB17_001305"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAP1979257.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLFLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(21464..21787)
                     /gene="soxS"
                     /locus_tag="ISB17_001306"
     CDS             complement(21464..21787)
                     /gene="soxS"
                     /locus_tag="ISB17_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAP1979258.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            21873..22337
                     /gene="soxR"
                     /locus_tag="ISB17_001307"
     CDS             21873..22337
                     /gene="soxR"
                     /locus_tag="ISB17_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAP1979259.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            22884..24233
                     /locus_tag="ISB17_001308"
     CDS             22884..24233
                     /locus_tag="ISB17_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1979260.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            24384..26033
                     /locus_tag="ISB17_001309"
     CDS             24384..26033
                     /locus_tag="ISB17_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAP1979261.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(26069..26953)
                     /locus_tag="ISB17_001310"
     CDS             complement(26069..26953)
                     /locus_tag="ISB17_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1979262.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            27057..27467
                     /locus_tag="ISB17_001311"
     CDS             27057..27467
                     /locus_tag="ISB17_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP1979263.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27460..28149
                     /locus_tag="ISB17_001312"
     CDS             27460..28149
                     /locus_tag="ISB17_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP1979264.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(28188..29837)
                     /gene="actP"
                     /locus_tag="ISB17_001313"
     CDS             complement(28188..29837)
                     /gene="actP"
                     /locus_tag="ISB17_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAP1979265.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(29834..30148)
                     /locus_tag="ISB17_001314"
     CDS             complement(29834..30148)
                     /locus_tag="ISB17_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAP1979266.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(30358..32316)
                     /gene="acs"
                     /locus_tag="ISB17_001315"
     CDS             complement(30358..32316)
                     /gene="acs"
                     /locus_tag="ISB17_001315"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAP1979267.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            32708..34144
                     /gene="nrfA"
                     /locus_tag="ISB17_001316"
     CDS             32708..34144
                     /gene="nrfA"
                     /locus_tag="ISB17_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAP1979268.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            34189..34755
                     /gene="nrfB"
                     /locus_tag="ISB17_001317"
     CDS             34189..34755
                     /gene="nrfB"
                     /locus_tag="ISB17_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAP1979269.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            34752..35423
                     /gene="nrfC"
                     /locus_tag="ISB17_001318"
     CDS             34752..35423
                     /gene="nrfC"
                     /locus_tag="ISB17_001318"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAP1979270.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            35420..36376
                     /gene="nrfD"
                     /locus_tag="ISB17_001319"
     CDS             35420..36376
                     /gene="nrfD"
                     /locus_tag="ISB17_001319"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAP1979271.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            36396..38114
                     /locus_tag="ISB17_001320"
     CDS             36396..38114
                     /locus_tag="ISB17_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP1979272.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            38107..38490
                     /gene="nrfF"
                     /locus_tag="ISB17_001321"
     CDS             38107..38490
                     /gene="nrfF"
                     /locus_tag="ISB17_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAP1979273.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            38487..39083
                     /gene="nrfG"
                     /locus_tag="ISB17_001322"
     CDS             38487..39083
                     /gene="nrfG"
                     /locus_tag="ISB17_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAP1979274.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         9579 a         9360 c        10429 g         9749 t
ORIGIN      
        1 cctctccaaa tgaatttctg ttgagtcgaa gaaacttaag tacaaacacg ctaatcagga
       61 atcttcgaga gttgtctgtt ttgtatcgat ggctggagga cagacaaatc gatctgagag
      121 caaggctgaa aattggcaca ctattcacag aggctgaatt cagtggtggt ctgatagagt
      181 tcctaagaaa agatatggaa tctgaagata aggttgtctc gcctcataca ttcaataatc
      241 gattagcaac gatccgacag tacttcgtat gggagctcga tgtttatatt tcatctttac
      301 cactaagtga ttcccaatat gaggttcttc aggctgcaag gaagcgccta atgggatgga
      361 ttgatcgagc ctttataaat actccaaggg ctggaagtat tccgaggaag tctctaacca
      421 atgacgaagc cgagttctta ctccagtgtc tgaatcctga aagtccagat tcgtttgcgc
      481 atttcgagcc tgttaaattt agaaacttcg cggcggtgtc tctgatgctt aattgcggac
      541 ttcgccctgg ggaattgctt agtttgcgtg tagaggatat tcagattggc tcgatctcgt
      601 cggtgactgt taaaagaagg tccccggacc cgctcgacaa gagaaaacca agacccagtg
      661 tcaagcgcaa tggcagggta ttgcctttag aaggacatca ctttgtgcga atgctggatc
      721 gctacatagt tgagtggagg gagattttgg agcagaaatc gagcagtgaa accgactatt
      781 tgatcttaag tgatgaaggc gaccctctgt ctcattcgtc tcttacgcaa cttttttctc
      841 gtctacggag tgaatattct ggtttgcttc cggagattct tacacctaaa tctctgaggc
      901 ataccttttc tagccgtatg gaacaggtac ttagggcggc aggcatggaa gaggatagaa
      961 ggaagcaagc cttggctatg ttgcgaggag actcaagttt ggagtcccaa tctgtgtata
     1021 tcgctcaaga ggttgaagag caggctcgtc gggcactttc tgactatcaa aagaaattga
     1081 ttacagggtt caataaatga atttgctctt gcataaagcg ccccagattt ctgactctgc
     1141 gctagagtta ccctctcaat tttttactcg agagggtgta aaagttgaga ttactggtga
     1201 tagatggaca tttcctcttc tgtatcggca tagcaggctt gatttttctc gagttgaaaa
     1261 cgacacgctt aaaacttcga ttaagcgctg tattattgat caggcatcaa gggtgtcaac
     1321 tcatgcaggg tgccagtact ggagtgatat atccgctgca atagtctctc gccaaaaaaa
     1381 gcactccttg ttgcccgaca tttccgctga tgaatttcaa gaacgtctga ttgctgtaat
     1441 ggaagaggca atcagtcagg cgcgaaataa gcacaggctg tgggcgttgt accggccaat
     1501 tcagtggtac atctggtgct ctgaaaacta ttcggagctc gggttctcga tagcctatgc
     1561 acaagagctc gattctctag aaattccagg caaccctaag ggtgaggcgg ttcgtcagga
     1621 agatccagaa tgtgggccgt tgcataggac gctggagctt cagcctataa tcaatgcgct
     1681 taagcaggat caaagtaaag tctacgagca tttgcagcaa aaaacagctg tggctctatc
     1741 tatagctttt ggccgaaatc ccgcgaattt aacattttta aaagaaactg atttagttga
     1801 cctgacaccg ggttcatcgc caccgtgtta tgtcttgaaa atgccaagaa taaagaaaag
     1861 gcagttggct ccaagggatg acatgatcga tgagtattta gagcctgaga tcgcaaagca
     1921 cgttttggag cttatagatg cgtccaggca taggaagctt ctaattgagg tagatggaaa
     1981 ggcagtagaa gtcaccagac ctctatttat aaaccccaga aaaaatgctt atgcgattga
     2041 ttcaaagcgt tgggatgagg cttataacat gctgtccact gggatagcga accttgtgaa
     2101 agcttttata aaacgtcaca acattatctc gccgcttact ggcgaattat tgcatgtcac
     2161 gccaaggcgg ctgcgatata ccttggcaac tgggctggct gctgagggaa tcagtaagcg
     2221 tgagctggcc agaatactgg accatacaga tacccagcac gtgaatgtct attttgagat
     2281 ggctggaaaa atagttgaac acctagacaa agcaaccgcg aaaggtttct cgaagtacct
     2341 caattttttc aaaggcaggc taatcgatag tgatgaagat gctgtcaaag gtgagcgaga
     2401 tgacaagcat ctaacgtttg tagatgatca gaatcctgct gatcaagctg atattggcgt
     2461 gtgtggtgaa tctagcgtct gccaccttaa tcccccctac tcatgttatt tgtgccccaa
     2521 gtttcagcca tatcgtcatg ctgatcatga gcatgtcttg gagtgcctgc tggccggtcg
     2581 cgaagagcgt ttgaagaagt acgagaatgc gcgattgggc atgcagctcg atgaggtaat
     2641 cgcagcggtc gcacaagtag ccaaactctg tgaggaaggg gggcatcatg tctgatggtc
     2701 gctctaatcg caaggccaag gtcgtccctt tcgttgatcg tcttgagcgt gacaggaatg
     2761 aaaatctcaa ctctttagtc agcaaagcaa agctaatgaa gctggaaggg tttgagtcgg
     2821 ttgtttggga ggaccccgag tggcaagtcg atgcaggtcg cctagtcaag ttaactggta
     2881 aaaatacgaa atcagcctct tttggttttt tgctatctcc gaagctgggt tcagcgccac
     2941 tagacggttg ctgggagctg gttgccaaag cattgtttgt tctcaggttc cacagaaaat
     3001 atcaatcagc accgaatcag cgcagtttca ttaccgctat tggatatgtc tcatttgcag
     3061 ctaaacagtt agggcaggaa ttagttgggc ttacgccaga ggctctggat aatgcgtctg
     3121 gtttaatttc aaaacactac agtgaaacca ctgcctacaa ccttcacaag catgttgcag
     3181 agttcgcagc gcattgtgat tcgaatggtc tatgtcgggt tttatttcag tacaaatacg
     3241 ccaaaatgaa gcgtcccgcg agcacgggag ggataaatca caaaaggttg gatgatccag
     3301 aggctctgga tacgaagtcg gacaaacttg tcgatcccgc tgtgtttcga gtcattggcg
     3361 aactctactt aaacgttccc aaagagcata agtatcgctt ctatattctg atgttgacgc
     3421 tacttgcttg cacaggaagg cgattcagtg agatttcact attaccaaat cagcaattgt
     3481 cttctgatga agaggggatt gcctatatcg aatactttcc gcggaaggca agtcgtggtg
     3541 acgttttcac acctaagcgt agactttact tgccgtcaga agtgaccccc atcgttggtg
     3601 atgtgctcgt tgagttagcg gaaattacat ttgaagcgcg tttaacagcg gaagaaattc
     3661 acaaagttgg tgagcctgat ctaaggtttc tggatggcat acctgaagat aaaaagctct
     3721 acgcggctga tttaactgaa ttggggatca gtcatactgt tttgggcaca actggctggc
     3781 tcaggaagca aaacctcgcg tggccagatc atagcgcatt aaccaagcag ggaattaagc
     3841 ctgcaaatcc cattttttac acttacaaaa gtggattggt tgaatactgt ttccgtgatt
     3901 tctcgggagc atatttatcg gctcttcata cggaccaatt tggtaaagaa tactacctaa
     3961 aagatctttt attcattcgt tctcttggac tttccagcgg aagctacgct cattggctgg
     4021 ccacaagttg tagccagtcc atgtttacta catttctccg ctatttccct gcgctcgccg
     4081 ccgaatacgc ctccagcagt atcgaggtgg acttcaccag tcatcacttc cgccatacgc
     4141 tcaatactct gcttgatgag ggcgggttaa gtgacctgct gcaaactgaa tggtttggtc
     4201 gaacgaaccc aagagacacc aaagcctatc agcatacatc ccgtgaaaaa cgagcactga
     4261 tgctccgtga agacatcaaa aaaggtttgg ttggaggtca gttagctgag caaatcaaag
     4321 tggttccggt agaagtacag gatgccattc tgaaagcacg aattcaggcc gtacatgatg
     4381 tcggtacagg tatctgcatc cacaatttct ctcaaacccc ctgcgagcgc catttgcagt
     4441 gttcggcgga ctgtaaagac tacgtgtggg ctaaggatga taagggtcgt ctggacgagc
     4501 aaaaacgcca atatgcgctt actgctctag cccgaaaaaa agctgaacag cagttggata
     4561 gcactaagcc aaaaaagagt gctgactggc tggcacacaa cgataaaaaa ctgaaaacac
     4621 tggctgccca actggccgac aacggggtgg agcactttga tcccgaacaa tatttacacg
     4681 aggtagagca tggttaagcg cttcagacgc atgtccgaca gtgacatcaa tacaatcgtt
     4741 gccgatttgg atcgctgggc gttgggtgag cttggatcaa aactcacctg ggctgtgcta
     4801 gaagagcgct ttggctttag tcgccagtcg ctccaagcta aatctgagat caaggccgcc
     4861 tatgacaacg ccaagcgagc attgtcgggc ggattggtga aaaccaaaga gcaggccact
     4921 aaagaggccg aagagcttca ggtagaggta gatcgcctca aagcggagct agatgcctac
     4981 aagcgaaaag aagagcaatg gctgcgccgc tggcagcaaa tcgcctttca tgtgcggcag
     5041 aaagggatac agatggctag cgttgataag gccccaccag aaggggctga gctgccctca
     5101 aacactgaaa ccgcgcagat actccggccg tttgataagg aagttccacc ttcagggagg
     5161 gtttagtagg cactagtctg gagattttaa acttgacaaa acttgactgc ggcatatact
     5221 tcgcgcatgc aaaaaagact aaatacagat aaagcacagc aggcgatcga taaggcaggg
     5281 ttaacccaaa ctgccgtcgc ggatgctcta ggcgtaacca aagaagccgt ttcccagtgg
     5341 cttttgcaca agtcttttcc ccgacctaac aagctacttc agttaggcaa gttgttaaat
     5401 ctctcttttg atgatttggt catcaaggaa gatccgcacg ccccgaaagt ggcattccgc
     5461 aaaatgcgcg gtaccaaaac caaggattac catatcgaaa aagcgcaaga gattggtcgc
     5521 tttttgcgtc acctcgtgcc ctatttgccg tttgacacct ttgaaatgcc gccggtactc
     5581 aagtcgccga gctgtgatta tgactatctt cgaacggtaa cagcgaaagt cagagaagat
     5641 attaatcttg gcccggtgga gtgtgtggat ttcacgcatt tgattcgccg cttcagcgaa
     5701 ctacaggccg ttgtggtccc agttatgtgg ggagcaaagc agcgtcatga aaatgcagta
     5761 cacatacacc ttcctgactc gggcagcaca tgggtatacc tgaatctgga taccaatacc
     5821 catgacttca agttctggat ggcacatgag ttggggcact gtctttcgcc aagccttgag
     5881 ggtgatgacg cggaagactt cgccgatgct tttgccgcga gcttgcttta ccctcatgaa
     5941 ttagcggagc aggcctacga agtgttggtt aaaaagccgt cgactgctgc aaagatcaat
     6001 acgttaaagc agatggcaga gctgcaggta atttccccaa atactatcct tcagcaagtt
     6061 aacaagtacg caaagcggct tgatagagct gaaattataa tgggtaattc cttctttggt
     6121 gcggtaacca aatttaacca gcaattccta aatatcagcg aagccctgtt cggtgataat
     6181 gagctggacg aatacggcaa gccaagcgcg cgcgactaca tcgaaaaagt agaaggcgct
     6241 ttcgaaactc ctttcttcga tatgcttcga caatatctga aagagcacga taaagggccg
     6301 ggttatgttc aaactgtgct ggatatgcca ctcctcgatg cacagagtat ccacgcagag
     6361 ttgacctgat gccgcaatcg aaaatcctcg ttgataccaa cgcctaccta aggttggcca
     6421 aaaccgtacg ccccttactg tttgtgccct ttggtgacaa cgagtattgc ctgtacattc
     6481 tgcctgaact caatgaagag ctggcgggta ggaagctgca aagtaagttc ccttgggtag
     6541 atgacgagga attcgccgag aatcgcaaac acttccccca aatcggcaaa aaacaaaaga
     6601 agtccattca gcaaaccttc gaatacgtgt gggatcatgt gcaaaccgag ttgccgggtc
     6661 cgtccaaggt ggatacttgg tatgtcgcct atgcattgga gttgggtgta ccggtggtga
     6721 cggacgatca ggatatgact gatttggcca aagccttcga tgcgcaggtc atgccaacct
     6781 tagagctgct caaaatcatg ctggactgtg gccatacaga catgaagacc atcaatggcc
     6841 tggttgagta ctggaagtac tttgcagaca tgcctgccaa cttcaagccc gactaccagc
     6901 gcctgtttgg tgatcaataa gcctgaaagc ttttcttact tgatctccct cccagatcct
     6961 atatattgac agtcttgtac tttttcgaca cctccgacat agctgagata cctcgtcatg
     7021 agtgacccca tattgaccat caaagacgtg gccgattacc tcaaggtgaa tgaacggacc
     7081 atataccggt tggctgcaag cagcgaatta ccgggcttta aagtgggtaa ctcctggcga
     7141 ttcaaacaga gcgagctgga gcaatacatc gccgcccagc acaaccgcgc cggtgtcagc
     7201 gaagcaatga aatcaccaaa cagtaaatca gaagataact aggaatccgg ccccatggca
     7261 caactggaaa acatcgaagc aattgaaaaa cgcctgtgga aatcggctga taccctacgc
     7321 gccaactccg agctggccag taacgaatac tttttgccgg tgatggggct gatcttcctg
     7381 cgacatgctt acagccgcta cttatcgcta aaagacgaga taatcgcaaa cctgcccagc
     7441 cgtggcggta aaacccgcga gcttacaaaa gaagatttct ctaaaaagag cgccatctat
     7501 ctcaagccag aagcacagtt cgactacctc attgctctga cggatgctga taatcgcgcc
     7561 gaagccatta ttcatgccat ggactccatc gagaccgatt acaccaacct ccgtaaccag
     7621 ctgccgaagc aggaatacaa caacattccc aatgatgtat taggtatgtt gctgcgtacg
     7681 ctcaacccag aagagcttaa aaaagccacg ggtgatatct ttggccgtat ctatgaatac
     7741 ttcctcaccc agtttgccga tcagggtgcg cacgatggtg gtgaattttt cacgccggta
     7801 tcgttagtgc agttgattgt gaatgtgttg gagccagatc acggcaaaat ttttgacccg
     7861 gcttgcggtt ccgggggcat gttcgtacaa agtgcccact ttatggagcg gcacgcgcaa
     7921 gacccgcatg aactcacctt ttacggccac gaaaaaaatc gcgtgaccac acgcctggcc
     7981 aaaatgaacc tggcggtaca cggccttgaa ggcaacgtgg aaggtggtga agccgccatt
     8041 acctactaca acgatcccca cgaaggcctg ttcggcactg tcgactatgt gatggccaac
     8101 ccgcccttta acgtggatga agtcgacgcc gataaaatca aaggcgataa gcgccgcttg
     8161 ccgtttggct tgccgggcgt aaataaaaac aaaaaagtca gtaacgccaa ctacctgtgg
     8221 attcagtact tttatagcta tctcaacgat tcgggccgag caggttttgt catgtcatcc
     8281 caggcttcca gtgctgggcg ggacgaagct aaggtgcgcg agcagttggt caaaaccggt
     8341 catgtggaca tcatggtcga tattcgcggt aactttttct atacccgcac cgtgccctgc
     8401 cagttgtggt ttctgaataa aaacaagccc gcgcacctga aagacaaggt gttaatgctg
     8461 gatgcacgca acgtctaccg caaagttacc cgcaagattt acgacttcag ccccgagcag
     8521 caacagaacc tgactgccgt ggtctggcta taccgtggcg aaggagagcg ttttgttgag
     8581 ctggtgcagc agtatatcga caagtcaatt ttcgaagctc gctcctgtga acaatctgaa
     8641 gcgctagcct gcgagccaat accagacttt atcactcagt tgggaaaatt cggccaagcc
     8701 tttcaaccct ttatggacaa actggagcag ggcggcgtga gtgttgagcc ttatgtcgat
     8761 tttctgaatg ccaaaggctc ggtcgaaggc ggttggtccg cgtttcaaat cctgaccaac
     8821 gacctgcaga aatggtggaa cgaatacgac tataacgatg ctgccagcct gctggacttc
     8881 atcgataaag atacctgcct aaaagacctg gccgaacaaa gccgcaacct gattaaagaa
     8941 atcgacctgg cttataagct ggcaacccgc gtgattgaac tggccgaagc caatgatgca
     9001 aaagacagcg aactctggga taacacgctg ttaaatggcc gcagtcgcag caacttaaag
     9061 aaaaccgccg atgacgcccg caagctggcc gtagagcaac tcaagcaggt acgctacttc
     9121 tacaagcaag cccactggct gttaagccgc ttcccggaag gcaagctctg tgatgtggaa
     9181 ggcctggtca aactggtaga tatcaaagcg atcgaagccg ctgactggag cctgacgccg
     9241 ggccgctatg tgggtgtggt gccggaagag gtggatgaag acttcgattt tgaagaaacc
     9301 ctgcatgata ttcatattga actgcaaggt ttgaattctg aggccataga gctagccgaa
     9361 aaaattgccc gtaactttga ggagttgggg gtatgagctg gcaaagcgaa cctctgtcag
     9421 cgttaaccct caaaattggt agcggctcaa ctccaagagg tggtgattct gtctatatat
     9481 ctgaaggtac aacgttaatc cgtagccaaa atgtatataa caatgaattt tcaacggatg
     9541 gcttagttta tatcgatgaa aaaacagcag aaaaaatgaa aggcgttagt gttgagaagg
     9601 aggatgttct tgtcaacatt accggcgatt ctgttgcacg atgctgtttg gtccatgatg
     9661 cagtactccc agcaagggta aatcagcacg ttgcgatttt aagaacaaat ccaaagaagc
     9721 ttctgccaca ttttcttgct ttctacatgg tatcaccctt catgcaggcc aagatgcttt
     9781 cttgggctgg tactggtgga accagaaaag cactgacaaa aggcatgcta gagggatttg
     9841 aaataccact cccccctgtt gatgttcaag ataaaatcgt ccaaaagatt aagacttata
     9901 acgacctcat cgaaaacaac cgccgccgta ttcagttgct ggaagaatcc gctcgcctgc
     9961 tgtatcagga atggtttgtt cacttgcgct tccccggaca taagcaagtt aagattactg
    10021 atggtgtgcc ggaagggtgg tcaaaggaac ctttagagaa cttactggtt cttcagcgtg
    10081 ggttcgactt gcctgtttcc aaacgtatag aaggaagcgt tcccatctat gcatccacag
    10141 gtattaatgg attccataat gtggcgaagg taaaaggccc tggtgtcgtc acgggaagat
    10201 ctggttcact gggaacggta atgtatgttg ctaaagacta ttggccactc aatacgacat
    10261 tgtgggtgaa ggaattcaaa aaagcgtcac ccatattcgc aacatatttg ctaagagcaa
    10321 tgaagcttga aggctataac ggcggtgcgg ctgttcccac attaaatcga aatgacgttc
    10381 ataaggtcga tgttctgtgc cctgaatcga aattaatgaa tgaattcgaa gttcaggtag
    10441 aaaacatttt caaacagata gacaaactaa aagaatataa cgagaagcta gcccaagcac
    10501 gtgacctgct actacccaaa cttatgagcg gcgagctaac cgtgtaaatc aaggagtgat
    10561 catgaaccgt gcccaagccc aacaattatt acaaaccgct ctagctaatc ccgccgccga
    10621 attccgtgat ggacagtggg aggccattga tgcactggtg aatcatcggc agaaactgtt
    10681 ggtggtacag cgcaccggtt ggggtaaaag ctcggtttac tttatcagta ccaaaatttt
    10741 ccgcgatcgt ggtatggggc caaccattat cgtatcgcct ttgctggcct tgatgcgtaa
    10801 ccaaattgaa tctgctgctc gacttggcat agtcgccgag acgatgaatt caaccaatac
    10861 tgatgattgg cgttcggtaa ctcagcggat tctgaataat caaatcgact gcttgttgat
    10921 ttcccctgag cgcttggcta atgacagctt tattgaaact gtgttgcagc ccattgccga
    10981 ccgcatagcc ctgatggtga ttgatgaagc gcactgtatt tccgattggg gtcatgactt
    11041 ccgccccgac taccgtcgta ttgtgaatat tcttcgccag ttgcctgcca atacgccggt
    11101 gctgggcact acagccacgg cgaataaccg cgtggttgcg gatattcaaa ctcagcttgg
    11161 aaatattcaa attcaccgtg gtccgttgat tcgggaaagt ctctccttgc agaccatggt
    11221 gttaccggat caggcatctc gtttggcttg gttggcgcag gtgataccca cgttgccggg
    11281 tacaggtatt gtctacgtac tgactcagcg tgatgccgat ttggttgcaa aatggttgaa
    11341 tcagcagggg atatctgcac tggcatatta cagtggcgta acgcatccta attttgttaa
    11401 ttatgcaggg aaagaagata cagatctcta ccgccaattt ctggaaactc agttactaga
    11461 aaacaaaatc aaagttcttg ttgcaactac tgcattaggg atggggtatg acaaacctga
    11521 cctaggcttt gtgcttcatt accaagcccc aagttcgata gttgcttact atcaacaagt
    11581 aggtcgcgcc ggtcgtggaa tcgaaaatgc gattggcgta ctaatgtcag gtactgagga
    11641 taacgacatt catgctttct ttcgccgaac agcctttcca agtcaagatg atgttcagcg
    11701 tctacttgca gcattagaag atgcagacgg attgtcactt gagaagttag agaaagaagc
    11761 aaacctgccg caaaagcgaa tagagcatgt tttgaaatat ttgcgtactg agagtccatc
    11821 gcctgtcatt gcgattggaa agacttggtt tagagctcct gttcgctacc aaatggatct
    11881 agagcatatt cgtaaaatta cagagattcg tgagcgcgaa tggagcgaga ttaatgatta
    11941 tctcaatact tcaggttgtt taatgttggc tttacgtaat gcgcttgatg acccttcaca
    12001 ggaaacttgt ggcaaatgtg agaactgtat tgcggagcca gtgcttacca aagttttcga
    12061 tcaagaaatt ggaataagag caactcagtt tttaagacac tcagaagaaa ttattaagcc
    12121 ccgtaagcaa atagcagctt cgaatgatga ggcagcgaga acatttgttg aatatgaatt
    12181 tccgcgtaat ctcggtgact tagtcgctca agagggccgt gttttatccc gctggggcga
    12241 tgccggttgg gggcggacgg ttgccgacaa caaacatgcc gggcgtttca gtgatgagtt
    12301 ggttgaagcc atggcagaaa tgattcagca gcgttggcag cctaaccccg cacctcaatg
    12361 ggtatgttgc gtgccgtcgc gtaatcatcc tgaactggta ccggattttg ctcgccggct
    12421 ggctgcacgc ttggggctgc cgtttgtgga tgcagttagc aaagtcaaag acaatcagcc
    12481 gcaaaaaggc cagcagaatc gcttccacca atgtcgcaat ctggatggtg catttgctgt
    12541 gactcagctt tatcaggggc aaccggtttt gttagtggat gacatagtgg actccggctg
    12601 gacgctaacg gtcgtcgcgg cactcctcca gcaagcaggc agcggcgtgg tttaccccat
    12661 cgcgctggct tcatcttcgg tgaaagactc atgaatgtat cccgcttttc atcagctgat
    12721 ggctctttac cagctaacgg ctctttgtca cccacggcgc aagcgacctt gctgttgacc
    12781 agctatttct caaaagccag tagcgaaagc gcaaagccgc tgaccaatgc cgaatggggt
    12841 cgctttgcct tgtggttaaa agaaaagtcc accacaccag ctgatctgtt ggtttccgac
    12901 ccgaaagctt tgctgcaagg ctggcacgat gcgcgtatca gtgcggagcg catcattgaa
    12961 ttgctgagcc gtggtcatag cctcgcactg gccatggaaa aatggcaacg ggcaggcctg
    13021 tgggtggtca cccgttccga cccggaatat ccaaggcgtt taaagcagca gctaaaaaca
    13081 gattccccgc cagtgctgtt tggttgtggt aacaaagcct tgttgagtgc cggtggcttg
    13141 gcggttattg gctcgcgcaa tgccagtgag tctgatttgg cttttaccga ccaagtgggg
    13201 gctaaagcag cggcagaggg tattgccatt gtctctggtg gtgctcgggg tgtcgatgaa
    13261 acggcaatgc tgggggctat gaaacaaggc ggtgtggtga tcggcgtgat ggccgatagc
    13321 ctgctaaaag cagcgaccag cagtaagtgg cgtaaaggtc tgatgaatgg ccatgcagtg
    13381 ctggtatcgc ctttctaccc cgaggcgggg tttagtgcgg gtaatgccat ggctcgtaat
    13441 aaatatatct actgtctggc agacagctct ctggtgattc actccggtaa aaaaggtggc
    13501 acgctcaacg gagcagaaga aaatctgaaa aagggatggg tgccgctgtg ggttaaacac
    13561 acgacggaca aggatgccgc caatgccgat ttggtcacca aaggtggccg ttggctggag
    13621 gctgataata ctcaagcctt caagattgcg gaattaatgt gcaatgaagg tgggcgtgtg
    13681 aatcaggtga aggaacaaat gggcgatctc ttttccatgc ctgcacagcc cgaattgttt
    13741 tttgagcctg aatctaatcc tgagccagat tctgggcctg aagaagttat agagtcagag
    13801 cgcgagatag tcgttagcga aagccagaca aagattcagg ctattccaga gcagactgcc
    13861 gaaagcgaag ctgccccatc aggccctgtc gatttctatc agatgtttat ctccgaactt
    13921 caacacttgg ctgaaaagcc agtaaccgtt gatgcgctga ttgaaagcac acagcttcat
    13981 aagtctcagt taactgagtg gctgaaacgt gctgttgatg acggggtggt gaaaaagctc
    14041 aatcgccctg cacgttttca agtcaagaac aagaaataga aggatttgct catggccatg
    14101 acagaagaaa ccctggttca ggaagtcact gcagactact tgctgaatca gttgcagtgg
    14161 gatgaatctg tcctgggtat gtatgaaaag ctgggcaagg agggtgattt aggtcggctt
    14221 tcagagcaag aggtggtgtt aacccgctat ttgggcgaga aactgataga gctcaaccct
    14281 ggcttgccgg atgtcgccta ccaagaggcg ctgcgcgtgg tttgtgagat gccagcatcc
    14341 accaatattg ttgctgtaaa taaggagaaa tactcccttc ataagaatgg cgtagaggtg
    14401 agcttccata acgaaaaagg tgaacgggta aagaaacggc ttcgcctgtt tgattttgac
    14461 aactacgaaa acaatcactt cttgctggtc agagagtttt ggatcaaagg cgatatttat
    14521 cgtcgcagag cagaccttgt gggctttgtg aatggtattc cgctgctgtt tatggaggtg
    14581 aaaaacgttc acaaggatat ccgtgctgcc tatgagcaaa atcttgcgga ctacaaagac
    14641 accgtcccgc acctgtttta tcacaacgcc tttattatct tgggcaatgg tattgacgcc
    14701 aagattggct ccgtttccag taaatttgag catttcaatg actggaagcg tttgaatgaa
    14761 agtgagcctg gtgtggtgga tatggaaacc ttgttgaaag gtacctgctc caaagccaat
    14821 ctcatgaaca tttttgagaa cttcactctg tttgaccaga gctcgggccg cctggtaaag
    14881 attattgccc gcaatcacca atatcttggt gtcagccgtg ccattgatgc cgtgattaat
    14941 cgtggcgagc ggatgggtaa gttgggagtt ttctggcata cccagggctc tggaaagtcc
    15001 tattcgattg tctttttcgc tcagatggtg catcgaaaac tgggtgggaa ctataccttt
    15061 gtggtgctga ctgaccgaga agatctcgat acacagattt ataaaacctt tgcaggctgt
    15121 ggcttggtcg acaacgataa agacccgtgc cgcgccgata gtggtaaaga cctgaaagag
    15181 ctgatcggcc agcaaaaggc gtttgtattt accttgattc aaaaatttaa cgaaaaagtc
    15241 gaccctaaaa agccttattc cgagcgcgac gacattatcg tcatcaccga cgaagctcac
    15301 cgcacgcaat atggcacgtt gtcgttaaac atgcgcaacg ccttgcccaa tgccagtttt
    15361 atcggcttca ccggaactcc attatttaaa gatgatgaga taaccaagaa ggtttttggt
    15421 gattacatct ctacctatga ctttcagcgg gctgtcgagg ataaagcgac agtgcccttg
    15481 tactacgatg cccgtggtga agaattggta tttaccgatg aagatggcaa cgagcatacg
    15541 gttgcggacc ccaaaggcat caatgagcgc atagcggaaa agctggatga gttggaaatt
    15601 gaggatgtag acgttcagca aaggttggag cgagagctga agcgtgatta tcacattatc
    15661 actgcaacca gccgactgga tcagatagca cgtgactttg tggctcatta tgccaacgct
    15721 tgggaaaccg gcaaggcgat gtttgtgtgc ctcgacaaga tcacttgtgt acggatgcac
    15781 aaattgatcg atttttactg gcaacaggaa attaaaacca aagaaaaagc acttttgtcg
    15841 gctacggatg agcaggatct agcttggcga gagcggcaat tggcgtggat gaaagaaacg
    15901 ctgatggcgg ttgtggtcag tgaagagcaa ggtgaagtcg ccaagtttga aaaatggggg
    15961 ctggatatca agcctcaccg ccagttgctt aaagcaggac ttgaactggc tgatggctct
    16021 cgactggaca tggaagaagc ctttaaatcc agcgaacacc cttttagagt cgccgttgtc
    16081 tgtgccatgt ggctgaccgg atttgatgtg cccacacttt ctaccctcta tttggacaag
    16141 cctttgaagg cgcatacctt gatgcaagct atcgccaggg ctaaccgagt agcggaaggc
    16201 aagaataacg gtttgatcgt ggattactgc gggatactga aaaacctgcg taaagcattg
    16261 gcaaccttcg ccggtgctgg tgacgagggt catggcggag gtgagggcga aaatgaaccc
    16321 gccaagccca atgaagagtt gttagaaagt ctgaacgagt cgatcagttt tgttgctgag
    16381 tttttacgaa agcatgattt tgaactaagc cggattatta cagaaacagg ttttgctaaa
    16441 aatgcagcga ttgctcaagc caaggaaatc atcaatcaaa atgacgaaac acggaaacgt
    16501 ttcgaggtaa tggccagaga ggtgttcaac aagtttaaag cctgcattaa cgtgccgggc
    16561 gttaatggct atcgtgatat gcgcgatgcc atcaacgtac tctataagag tttgcaggca
    16621 gataaagaga aagccgacat ctccgatatt atgaaggatc tgtacgagat tgttgacggt
    16681 agcatcgata ccacccataa agtcaaagaa ccgaaaccgg atggtgatcg aatttacgat
    16741 atcagcaaga tcgattttga acggctccgc caggagtttt ctagatcaga gcgaaagaat
    16801 actacagtgc aatcgctcaa gcatgcggtt gagaagaagc tagccaggct catgatgcag
    16861 aacccgttac gtaccgatta tcaagagcac tacgaaaaat tggttaagga atataaccaa
    16921 gaaaaggatc gcgttgttat tgaaaaaacc tttgaggcac tgttaaaact gaatgaagag
    16981 ctttctcatg aagagaagcg cgcaatcagg gaatgcttgg acgaggagtc tttggtattg
    17041 tttgatctcc ttagtaagcc gaatttgcag ccaaaagaga ttgccaagat caaacaagtc
    17101 gccactacgt tgttggcaac gctaaaggca gagcggctga aagttgcaaa ctggcagcag
    17161 aaagaatcga cgcgcgacgc ggttaagcag cagatatttg attttcttta tgatgaaaga
    17221 acagggttac ccgttgagca atatgaagaa aatgagattt cagatatcac tgaaagggtg
    17281 tttcttcaca tatatcgggc gtaccccgaa ctgccatcgc ccatttatgg gtgaagaatt
    17341 acgcggagtt acgcatggtt tgcgtaatta cgcatatgat gcataattgt gcgtaatttg
    17401 aggcaactct atcccaaacc tcaaataggc cggttacctg ccggatggtg ccttggtcta
    17461 atgagctctt agccggtgac ggcttgaaag cgggtgtaaa taccaaggtt tgacatagag
    17521 ttgatggcaa gagcgggaaa tggattttat ttcgccgaga tcttgctaaa atgcgattca
    17581 atgttcggta gaaacaacca gccttccaat acccgccttt ttgattttgg gtgttggagt
    17641 cgcctgagtt gatcaggtgc tgttgacctg ctccccgttg attagtacac cccgatgtta
    17701 gtaatgtctt cataagccac atgaggacat ccccatgaag aagcgttttt ccgacgaaca
    17761 gatcatcagt attctccgcg aagccgaagc tggggtaccc gccggtgaac tctgccgcaa
    17821 gcatgccatt tccgatgcca cgttttacac ctggcgtaag aagtatggcg gtatggaggt
    17881 gcctgaagtt aagcgcctga agtcgcttga ggaagagaac accagactca agaagctgct
    17941 tgccgaagcc atgctggata aagaggcgct tcaggtggct cttgggcgaa agtactgacg
    18001 acagaccaga agcgggaagc cgtgatgttg atgtgtgatg cgaccggtct gtcgcaacgt
    18061 cgtgcctgca ggcttacagg tttatccctg tcgacctgcc gctatgaggc tcaccgtccg
    18121 gctgctgatg cgcatttatc agggcgcatc actgagctgg cactggagcg caggcgtttt
    18181 ggctaccgtc gtatttggca gttgctgcgc cgtgaagggc ttcatgttaa tcataagcgc
    18241 gtgtaccggc tttatcacct cagtggtctg ggcgtaaaac gcagaagacg tcgtaaaggg
    18301 ctggcaacag aacgtctgcc gctgctccgt ccggcggcgc ccaatctgac ctggtcgatg
    18361 gatttcgtca tggacgcact ttccaccggt cgcaggatca agtgtcttac ctgcgtcgat
    18421 gatttcacaa aggaatgcct gacggtcact gttgcctttg ggatttcagg cgttcaggtc
    18481 acgcgtattc tggacagcat tgcactgttt cgaggctatc cggcgacgat aagaactgac
    18541 caggggccgg agttcacttg ccgtgcactg gatcaatggg cctttgagca tggtgttgag
    18601 ttgcgcttaa tccagccggg caagccaacg cagaacggat ttattgagag ctttaacgga
    18661 cgatttcgcg atgaatgttt gaatgagcac tggttcagcg atatcgttca tgccaggaaa
    18721 attattaatg actggcggca ggattataac gaatgccgcc cgcactccac gctgaattat
    18781 cagacaccgt ctgaatttgc agcgggctgg agaaagggtc attctgagaa tgaagattcc
    18841 gacgttacta actgagtgtt gtatctaatc gtgggggcag gtcactgtcg ctgaaagtgt
    18901 caagattcta tctttacgct tggcggctaa tacttccaca gtacctgccc aggaagttga
    18961 caatattggg cttaacttaa cagctttggc tttctttatg ataaaatcca atattttcaa
    19021 tgggttgaat ggtttgtttg ctgctttatg tgaactaccg ttctgatttg tctttaaaac
    19081 aatagggtat attgttttaa ggtggatgat taaagcatct gccagccata aaaaagaagc
    19141 ctccgttatg gaggcttcta catatcaagt caaaatcatt ggccattgtg gcggtgtctg
    19201 attcatctct tctatcatca ccgactcgat gttgtgccag atggcggtga tatccgccat
    19261 tgtgatgcca agcaacccca ttgcgaacca gcaggcggaa actacgccca aaccgctgct
    19321 gatcactgca agcccgacat aatcagcccg acgaaaacgg atgttgagcg tgctggccag
    19381 aaacatcatt acggcactaa gaagttgcca gcgaagaaga accacgccag tggtgagggt
    19441 agccatcaaa caattcctcg aaaaagagct gatgccctgg acagcgcggc gttgttcacg
    19501 ggaggcgtga tacactctgg ctatcgcggg gcttgcagaa cacaaaaatg aaacactctg
    19561 tttgtttcat gaattttgtg aactatatca caattggttg tttgttagcc agattaggcc
    19621 gtgactttta ttgccgtaca gattatgtgt tttttcagcg gcattaaggg tatatcttcc
    19681 cgccgcctct gcattcctga aggtgattaa tttttaagat caatgaatta tcgtcatcct
    19741 ggtgacgatt aaaaaggtct caattttata tcaggcaaaa gtgctattta taccgtaaga
    19801 tttatctaaa gacgtcggta gccagggttt caccttgcaa taaccgggta taaacaggca
    19861 gggaattgat agcaatgagt catcgtgcac gacaccaatt actggcgttt ccgggcatta
    19921 tctttttagt tctctttccc atcattctgt cgctatggat tgccttcttt tgggcaaaat
    19981 cagaagtgaa taatcagctc cgaacttttg ctcagctggc gttggataaa tctgagctgg
    20041 tcattcgtca ggcagattta gtgagcgatg cagctgaacg ctatcagggg caagtttgca
    20101 ctccagccca tcaaaagcga atgttgaata ttattcgtgg ctatctttat attaatgaat
    20161 tgatttatgc tcgtgataac cattttttat gctcatcgct gatagcgtct gtaaatggct
    20221 atacgattgc accggccgat tataagcgtg aacctaacgt ttctatctat tattaccgtg
    20281 atacgccttt tttctcaggc tataaaatga cctatatgca gcgggggaat tatgtagcgg
    20341 tcattaaccc tctcttctgg agtgaagtga tgtctgatga tccgacattg caatggggtg
    20401 tgtatgatac ggtgacgaaa acctttttct cgttaagcaa cgaggcctcg gcagcaacgt
    20461 tttctccgct gattcgtttg aatgatttaa ccgtacaacg aaatggctat ttatatgcga
    20521 cagtttattc gacaaaacgc ccaattgcgg ccattgttgc gacttcatat caacgtctta
    20581 taacccattt ttataatcat cttatttttg cgttgcccgc cggtattttg gggagtcttg
    20641 ttctgctatt actctggcta cgtattcgac aaaactattt gtctcccaaa cgcaaattgc
    20701 aacgcgccct cgaaaaacat caacttttcc tttattacca gccaataatc gatatcaaaa
    20761 cagaaaaatg tatcggcgct gaagcgttgt tacgttggcc cggtgagcag gggcaagtaa
    20821 tgaatccggc agagtttatt ccgctggcag aaaaggaggg gatgatcgaa caggtaactg
    20881 attatgttat tgataatgtc ttccgcgatt tgggcgcgta tctggcaaca catgccgatc
    20941 gctatgtttc tattaacctg tcagcctccg attttcatac ctcacggttg atagcgcgaa
    21001 ccaatcagaa aacagagcaa catgcggtgc gcccgcagca aattaaattt gaagtgactg
    21061 aacatgcatt tcttgatgtc gacaaaatga cgccgattat tctggctttc cgcaaggccg
    21121 gttacgaagt ggcaattgat gattttggta ttggctactc taacttgcat aaccttaaat
    21181 cattgaatgt cgatattttg aaaatcgata aatcgtttgt tgagacgctg accacccaca
    21241 aaaccagtca tttgattgcg gaacacatca tcgagctggc gcacagcctg gggttaaaaa
    21301 cgattgctga aggtgtcgaa actgaggcgc aggttaactg gctgcgcaaa cgcggcgtgc
    21361 gctattgcca gggatggttc ttcgcgaagg cgatgccgcc gcaggtgttt atgcaatgga
    21421 tggagcaatt acccgcgcgg gaattaacgc gcgggcaata aaactacagg cggtggcgat
    21481 aatcgctggg agtgcgatca aactgccgac ggaaaacgcg ggagaaggtc tgctgagaga
    21541 cataacccag gtccattgcg atatcaaaaa tcggacgctc ggtggtgcgc aactcaacgg
    21601 cggccagtaa cagacggcgc tggcgaatgt aatcgccaag cgtctgatgc gtcaccgtgc
    21661 ggaacatccg ttgcaagtac cactttgaat agccagattt ttttgcgacg acatcaatgt
    21721 taagcggctg gtcaatatgc tcgtcaatcc atgcgataag atcctgaata attttctgat
    21781 gggacataaa tctgcctctt ttcagtgttc agttcgttaa ttcatctgtt ggggagtata
    21841 attcctcaag ttaacttgag gtaaagcgat ttatggaaaa gaaattaccc cgcattaaag
    21901 cgctgctaac acccggcgaa gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt
    21961 tctatgaaag caaagggttg attaccagta tccgtaatag cggcaatcag cggcgatata
    22021 aacgtgatgt gttgcgatat gtcgcaatta tcaaaattgc tcagcgtatt ggcattccgc
    22081 tcgcaaccat tcgtgaagcg tttggcgtgt tgccagaagg gcatacgtta agtgcgaaag
    22141 agtggaaaca gctttcgtcc caatggcgag aagagttgga tcggcgcatt cataccttag
    22201 tggcgctgcg tgacgaactg gatggatgta ttggttgtgg ctgcctttcg cgcagtgatt
    22261 gcccgttgcg taacccgggc gaccgcttag gagaagaagg taccggcgca cgcttactgg
    22321 aagatgaaca aaactaaagc gccacaaggg cgctttagtt tgttttccgg tctttgtctt
    22381 tctctctatc ccgctggtac acaggagggt ttcccccgac gtcaacacac ctcattcgag
    22441 cacgtggtgg aggttccggt tggtgttgat gctttaattg tatgttaccg gcgtttcttc
    22501 gccagtgtaa aagtacactt tttagccgca atatttttgt catctcagac gattttttat
    22561 cgcaatcctg aacggtatac ggctcgataa cgctgcaatc ttgcgcatcg acgataacgt
    22621 ttgcgcatca attgcctggt ttttcatcgt caagacaata aaagagaaaa aagcagcaaa
    22681 cttcggttga aaaagccgct atgatcgccg gataatcgtt tgcttttttt accacccgtt
    22741 ttgtatgcgc ggagctaaac gtttgctttt tttgcgacgc agcaaattgt cgcaaacctg
    22801 gagcaggaag ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt
    22861 aaactctcag gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg
    22921 acgcctggtt taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt
    22981 taacaacgtt tctggcgatg gtctactcgg ttatcgtcgt tccaggcatg ttgggtaaag
    23041 cgggcttccc gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta
    23101 tcgtgatggg tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tctctgacgg
    23161 cgtttaccgc attcagtctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg
    23221 ccgtgttcct gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga
    23281 ttttgcgcaa cttgccgcac ggtgtggcgc acggcacggg gattggtatc ggtctgttcc
    23341 tgctgctcat tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc
    23401 ccgtcgcgct gggggatttt acgacctttc cggtgatgat gtcgttggtg gggctggcag
    23461 tgattatcgg cctggaaaaa ctgaaagtcc ctggcggcat tctgctgacc attatcggta
    23521 tctcaattgt cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc
    23581 cttcattgag cgatgaaaac ggcaattcac tgataggcag cctggacatt atgggcgcgc
    23641 tgaatcctgt agtcctgcca agtgttctgg cactggtgat gacggcagta tttgatgcca
    23701 ccggaactat ccgtgccgtc gccggtcagg cgaacctgct ggataaagat gggcagatca
    23761 tcgacggcgg gaaagcactg accaccgact ccatgagcag cgttttttct ggcctggtgg
    23821 gtgcagctcc ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa
    23881 ccggtttgac ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc
    23941 tctcttacct cgttccgggg tacgcaacgg ctccggcgct gatgtacgtt ggcctgctga
    24001 tgctgagcaa cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg
    24061 ttacggcggt attcatcgtg ctgacctgta acatcgtaac aggcatcatg attggcttcg
    24121 cgactctggt gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg
    24181 tcgttattgc cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatcttaca
    24241 taggattctg aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg
    24301 agcttttgcg atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa
    24361 aaaacatcgc aaacagggaa cgcatggaaa tcttcttcac catactgata atgaccctcg
    24421 tggtctcgct gtccggggtg gtcactcgtg tcatgccctt tcagatcccg cttccgctta
    24481 tgcaaatcgc catcggtgcg ctactggcgt ggccgacgtt tggtttgcat gtggagtttg
    24541 atcctgaact ctttttagtc ttgtttatcc cgccgttgct gttcgctgat ggttggaaaa
    24601 cgccgacccg tgaatttctc gaacatgggc gagagatttt cggcctcgcg ctggcgctgg
    24661 tggtggtcac cgtggtcggc attggcttcc ttatttactg ggtggtgccg ggcattccgc
    24721 tgatccccgc ctttgcgctg gctgcggtgc tttctccgac cgatgctgta gcgctctccg
    24781 ggattgttgg cgaggggcgc atcccgaaaa aaatcatggg cattttgcag ggcgaagcgt
    24841 tgatgaatga cgcctccggc ctggtgtcgt tgaagtttgc cgtggcagtg gcgatgggga
    24901 caatgatctt caccgtcggc ggtgcgacgg tcgaatttat gaaagtggca ataggcggta
    24961 ttctcgccgg ttttgtggtg agctggctgt atggtcgctc gctgcgattc ctcagccgct
    25021 ggggcggtga tgaacccgcg acgcagatcg ttctgctgtt cttgctgcca ttcgcttctt
    25081 atctgattgc ggaacatatt ggcgtttccg gcatcctcgc tgcggttgcc gccgggatga
    25141 ccatcacccg ctccggtgtg atgcgccgtg cgccgctggc aatgcgcctg cgcgcaaaca
    25201 gcacctgggc gatgctggaa tttgtcttta acggcatggt gttcctgctg ttaggtctgc
    25261 agttgccggg gattctggag acgtcgctga tggcggcaga aatcgatcct aacgtcgaaa
    25321 tctggatgct gtttaccgat attattctga tatatgcggc gctgatgctg gtccgtttcg
    25381 gctggctgtg gacgatgaaa aagttcagca accgcttcct gaagaagaag ccgatggagt
    25441 ttggttcgtg gactacacga gaaatcctga tcgcgtcttt cgccggggtg cgtggggcga
    25501 tcactctggc cggtgtgctc tctatcccgc ttctcttgcc ggatggtaac gtcttccctg
    25561 cgcgctatga gttggtgttc ctggcggctg gcgtcattct cttctcgttg tttgtcggcg
    25621 tggtgatgtt gcctattctg ctacaacaca tagaagtcgc ggaccattcg caacaattga
    25681 aagaggaacg tattgcacga gcggcaacgg cagaagtggc gattgtggcg atccagaaaa
    25741 tggaggagcg tttggcggcg gataccgaag agaatatcga taaccagctg ctcacagagg
    25801 ttagttctcg cgtcattggt aacctgcgtc gtcgcgccga tggacgtaat gatgtagaaa
    25861 gttcaatcct ggaagagaac ctggagcgtc gcttccgtct ggcggcattg cgttctgaac
    25921 gtgctgaact gtaccacctg cgcgccacgc gggagatcag taacgaaacg ctgcaaaaat
    25981 tactgcacga tctcgatttg cttgaagcgt tgctgattga ggaaaatcag taatctgcat
    26041 ggttggcggg cgagcccgcc aacccgactt actctggcgt taaccagtat ccttcacaac
    26101 agctcagcca cgcgtgggca gagtgggaaa ggtaaacgcc ctcacgccaa atcattccta
    26161 atttccagct tagttcgctt tccagcggta gccattttaa ggtgttttta tctaatcgct
    26221 ggcaaatggg ttccggcaat atggctattc cgacaccggc ctgaaccatc gcggcgagaa
    26281 aatcccattg cccgctgcga actgcaattc tcggcttaac gccatgttca tcgaataact
    26341 tcatcaattg cttacttaag gcaaaatctt cgttatagat aagtagagga tgttccgcca
    26401 atgtctccgg tgagacggca tcacattcaa gccatttacc tgaatgtggc gtcagtacac
    26461 atagtggatg actaaacagc gataacgttg tcaggccgct ttcttctact ggcagggctg
    26521 tcagtgccat atccagttca ccgttcatca ccgcttgttg taccgtcaga ccaccaaact
    26581 ccgaaatttt caattctacg ccggggtaac gctggcgaaa aaggccaatc ggcccggcca
    26641 tcaacatccc aaccatcggc gggattccca gccgcaaaac gccggtttta agctgattga
    26701 tatcgctaag ctcagcttcc agttgccgaa actcagccag taaagccagc ccgcgctcaa
    26761 atacgacgcg accagtatca gtgagaagca gccgacgacc atcgcggatc aacaatatac
    26821 agttcagctc atcttcgaga ttcttcagca tcttgctgat agttggttgg gtgacaaaca
    26881 acttctccgc tgcgcgggtg aagctttgct ggcgcacgac ttcgacaaaa taacgcagcg
    26941 ttctgatatc cataactatt ccctttgact atacctgcaa tgattttaat tcatttcagt
    27001 caggggcgag cgctctctat acttaccatt ctgttttatt ttcaggatat cctctcatgc
    27061 ccgtggcgct tcgccgcatt acccctgccg tagtgcaatc tatccaggta ttttttcagg
    27121 ttgtgctgta tgcaggttta tttatttttg cgcagtatct cgtctcctgg ctgcatttgc
    27181 cgctgcctgc caacctggtg gggatgatcc tgatgctggc gctgattgtt tgtcgcattc
    27241 ttcctctgca atgggttcga gccggggcgc gttggctatt ggccgaaatg ttgctgtttt
    27301 ttgtccctgc cgtggtggct gtggtgaact atgcacaact attactggtg gatggctggc
    27361 gtatttttgc tgtgatcgcg ctgagtacgg tgatggtgct gggcacgact gcctgggtag
    27421 tggaaaaagt ctatcgctat gaaatgcgca ggttaaaccg tggctaattt tcagctaagt
    27481 atgctctgtc tggcaattac gctagttatc tattttctta acaaacgcct ttatcgccgc
    27541 tttcgtaaat tgccattgat gcctctggtg ctgacgccta cgctactggt tatgctgctg
    27601 gtttttggca atatttcctg gcaaaactat attggcgaag cgcactggtt actatggcta
    27661 ttaggtccgg ccaccatcgc ctttgcggta ccagtgtatg acaatcgggc gatcattaag
    27721 cgtcactgga tgtcgttgac ggcaggcgta ctgacggcaa cggtggtggc agtgaccagc
    27781 tcagtgtggc tggcgcgatt gtttaccctg ccagatgaga tccagcgtag cctggcggtg
    27841 cgttcggtca ctacgccatt tgctttggca gcgagccagt cgctcggcgg acagcctgat
    27901 ctggtggcgc tgtttgtggt ggtcaccggt gtgtttggca tggcgattgg cgacgtgttg
    27961 tttttacgtc tctctattcg cgaagggatg gcgaaaggtg ctggattcgg cgcggcatct
    28021 catggtgccg ggactgcgcg gtcgtatgaa ctgggtcaac aagaaggcgt agttgcaagc
    28081 ctggtcatga tgctttcagg tgtattgatg gtattggttt cgccgctggt ggccaggatg
    28141 atgttctgaa gaaattcccc gatcaagcga ccggggaagg ggagagatta atgcgcgcgg
    28201 ccctgctcaa cgccaaagcc tgtctgggag cggataaact gcgcacggaa cagttcacgc
    28261 tcgcgcgcac cttccgctga attatccgtt gccgagaaga accagatacc gaggaatgcc
    28321 acgctgatag agaacagcgc cgggtattca tacgggaaaa tggctttttc gtgaccgagg
    28381 atctgtaccc aaatcgtcgg gccgaggatc atcagcacca ccgcagtaat cagtcctaac
    28441 cagccgccca gcattgcgcc acgggtagtc agtttcgacc agtacatgga aagcagaatg
    28501 atcgggaagt tacagctcgc cgcgatggca aacgccagcc ccaccataaa ggcgatgttc
    28561 tgattctcaa acagcacgcc gaggataatg gcaatcacac cgaggatcag tacggtgatt
    28621 ttcgataccc gcagctcttc acgttcggtc gcaccttttt tgaagacgtt agcgtacagg
    28681 tcgtgagaca ccgccgatgc gcccgccagc gtcagacccg caaccaccgc aagaatggtg
    28741 gcgaaagcaa ccgctgaaat aaagccgagg aacaggttgc cgcccaccgc attggccagg
    28801 tgaactgctg ccatgttgtt accaccaatc agatggcccg ccgcgtcttt atattccgga
    28861 ttcgcaccga ccagcatgat cgcgccaaag ccgataataa aggtcaggat gtagaagtag
    28921 cccataaatc cggtggcgta gaacacgctc ttacgtgctt cgcgggcatc gctgaccgta
    28981 aagaagcgca tcagaatatg cggcaagccc gccgtaccaa acatcagtcc tagccccaaa
    29041 gagagcgcgg agataggatc tttcaccagc ccgcccggct tcatgatgtc gacacctttc
    29101 gggtgtaccg ccatcgcttc gctgaacaga ttgttgaagc taaagccgac gtgtttcatc
    29161 accataaagg ccataaagct ggcaccgaac agcaacagca cggctttaat aatttgtacc
    29221 caggtggtcg ccagcatgcc gccgaacagg acgtacatca tcatcagcac gccgaccagc
    29281 actaccgcaa tgtgatagtt aaggccaaac agcagctcga tcagtttacc tgcgcccacc
    29341 atctgggcga taaggtaaag cgccaccacc accagagaac cacaggccga aagaatacga
    29401 atcggccctt gtttcagacg gtaagaggcc acatcggcaa aggtgtagcg accgaggtta
    29461 cgcagacgtt cggcaatcag gaacagaatg atcggccagc ccaccaggaa gcccagcgag
    29521 taaattaagc cgtcatagcc ggaggtaaac accagcgcgg agatccccaa gaatgaggcg
    29581 gcggacatat agtccccggc aatcgccagc ccgttctgaa aaccggtgat attgccgcct
    29641 gcggtgtagt agtcattacg cgaacgtacg cgttttgacg cccagtaggt aatgccgagc
    29701 gtaaacacga cgaaaatcag gaacataata atcgcctgcc agttcgttgg ctggcgctct
    29761 acggccccgc taatagcatc cgcggcgtta gctgcgaaag ggagtgtggc ggcaagcgcc
    29821 gtcagaactc ttttcatgat gcttgtacct catgcaggac ttcattatta agacggtcga
    29881 attcgccgtt cgcccgccag atgtagatac cagtgagaac aaaggagatc acaattaccc
    29941 cgacaccgac tggaataccg cgtgtgacgc tggtgttcgg attcagcggg gtgcccagcc
    30001 agccgggcgc gaaggcgatc agtaaaataa agccgatata aactgccagc ataataatcg
    30061 acaggatggt ggcaaaccgt tgccgttttt cgactaactc cctgaaatgc gcattgtctt
    30121 ctatccgctg ataaatcgta ccattcatca cagattctcc agaggtaatg tagggattgt
    30181 tttaattccc gctcccttat gggagaaggt taacgcttgg gtaacccttg ccgaatgtag
    30241 gccggataag gcattcacgc cgcatccggc gcttgttgtc ggatgcgatg ctggcgcatc
    30301 ttatccgacc tacagccgtt gccagatgta ggtcggagcg catccgacaa ttgtgggtta
    30361 cgatggcatc gcgatggcct gcttctcttc aagcagcttc tcgactacgc caggatcggc
    30421 aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc gcagaatacg
    30481 gcgcataatt ttgccggagc gggttttagg cagggagtcg gtccagtgca gcacgtctgg
    30541 cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt acagttctgg
    30601 tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac ctttaatatt
    30661 gtgcggaata ccgaccacag cggcttcggc aattttcgga tgcgccacca gcgccgactc
    30721 aatctctgcc gtccccaggc ggtgaccgga gacgttcagc acgtcgtcca cgcgcccggt
    30781 gatccagtaa tagccatctt catcacgacg cgcgccgtcg ccgctgaaat acatattttt
    30841 gaaggtggag aagtaggtct gctcaaaacg ttcgtgatcg ccaaacagcg tacgcgcctg
    30901 acccggccag gagtcggtga tcaccagact gccttcggtg gcccccttca gcgggttacc
    30961 ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg aaccggcttt
    31021 cagctcggta gcgccaggca gtggtgtgat catgaaaccg ccagtttcgg tctgccacca
    31081 ggtgtcgacc accgggcatt tctcgttgcc gatcttcttc cagtaccact cccacgcttc
    31141 cgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt cggtgccttc
    31201 gatcgcttta tcgccttccg ccatcagcgc gcgaatcgcc gtgggcgcgg tatagagaat
    31261 attgacctga tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc agttcggtac
    31321 gccttcaaac atcagcgtgg tcgcgccgca ggccagcggg ccatacagca aataactgtg
    31381 tccggtaacc cagcccacat cggcggtgca ccagtagatg tcgcccggat gataatcaaa
    31441 gacatattta aaggtcagcg ccgcgtacac cagataaccg ccggtagtgt gcagcacgcc
    31501 ttttggctta ccggtagaac cggaggtata gagaataaac agcggatctt cggcgttcat
    31561 ctcttccgcc tggtgctgat cgctggcttg ctcaaccagg tcgtgccacc acaggtcgcg
    31621 cccttcctgc cagtcaattt ttccgccagt acgcttcagt accaccacat gctctacgct
    31681 ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa tactacgccc
    31741 ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgaa ctggagtcga taatgcgtcc
    31801 ggcaacggct tccggcgaga agccgccgaa aatcaccgaa tgtaccgcgc caatgcgggc
    31861 gcaggccagc atcgcgaccg cggcttccgg taccatcggc atataaatcg caactacgtc
    31921 acctttttta atgcccagtt cgagcagggt attggcgaag cggcagacgt cgcggtgcag
    31981 ctctttatag ctaatatgtt tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt
    32041 acgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat tcagcgtgcc
    32101 gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtgtttt tcaccttctg
    32161 gtacggtgtg atccagtcga gaatttttcc ctgttcgccc cagaaggtat ctggtgcgtc
    32221 aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac ggtctgcgat
    32281 gttggcagga atggtgtgtt tgtgaatttg actcatgctt ttgttctcct tgtaggatgt
    32341 taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt tagtatttgg
    32401 gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt tttgaaggtc
    32461 agcaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgttg ttgaaaaaga
    32521 ggaagatacc gactaactct aaagtggtat tttacatgca cttacaattg attaaagaca
    32581 acattcatag tgtggttatt tgttacacat aggggcgagc aatgtcatga cagtgtaggt
    32641 gcggttactg tcgtgaaaaa caataaaaac cgccattgca acaatggctc aattcggatg
    32701 aagccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat tgattccttt
    32761 tttctttttc acttctgttc acgctgaaca aacggctgct cccgcaacac ctgtaactgt
    32821 ggaagcgaag aatgaaacct ttgccccgca gcatcccgat caatatctct cctggaaagc
    32881 tacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc tggtgatcct
    32941 gtgggcgggg tatcccttct cgcgcgatta caacaagccg cgtggacatg cctttgctgt
    33001 gaccgatgtg cgtgaaacct tgcgtaccgg tgcgccgaaa aacgctgaag atggtccgct
    33061 accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc agaaagacgg
    33121 cgaagatggc tacttccacg gtaaatgggc gcgcggcggc ccggaaatcg tcaacaactt
    33181 aggttgtgcc gattgccata acaccgcctc accagagttc gccaaaggca aaccggagtt
    33241 aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac cttttgagaa
    33301 agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg agtattactt
    33361 cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag tcgaaaatat
    33421 ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt cgaaaacgcc
    33481 aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc acggcaaaaa
    33541 caacgtgacc tgtatcgatt gccatatgcc aaaagtgcag aacgccgaag gcaaactcta
    33601 caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg cgaactgcca
    33661 tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt cgattaacga
    33721 cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga aagcggcgct
    33781 ggatgcaggc gcaacggaag ccgaaatgaa gccgattcag gacgatatcc gtcatgccca
    33841 gtggcgttgg gatttggcga tcgcttccca cggcattcat atgcacgcac cggaagaagg
    33901 tctgaggatg ctcggtacgg cgatggataa agcggcggat gcacgcacca aactggcgcg
    33961 cctgctggcg accaaaggca ttacccatga aatccagatc ccggatatct cgaccaaaga
    34021 gaaagcccag caggccattg gcctgaacat ggagcaaatc aaggccgaga agcaggactt
    34081 catcaaaacg gtgatcccgc agtgggaaga gcaggcacgt aaaaacggtc tgttaagcca
    34141 ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat gagcgtatta
    34201 cgttcgttat taactgccgg ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt
    34261 accgctaccc ctgcggcgca ggcatctggt gatcgctacg aagttaccca gcagcgtaac
    34321 ccggatgccg cctgtctgga ctgtcataag ccagataccg aaggtatgca tggcaaacat
    34381 gcttccgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg tcacggccag
    34441 ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga gccgatgtac
    34501 aaggttggag agcagaacag cgtctgtatg tcctgccatc tgccggaaca gttgcaaaaa
    34561 gcattctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg ccattccctg
    34621 catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa gatttgcgtc
    34681 gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt tccgttgctt
    34741 aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggt gtgctggccg
    34801 ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat ggggtgcgtt
    34861 acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc
    34921 ggaaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt agcgagccgc
    34981 agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag cactgcgatc
    35041 atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgcgacgct gccagtggga
    35101 ttgtcgatgt taatccggat ctctgcgtcg gttgtcagta ctgcatcgcc gcctgtccgt
    35161 accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat ttctgccgta
    35221 agacccattt gcaggccggt aagctgcccg cgtgcgttga agcttgcccg acgaaggcgc
    35281 tgacgtttgg caatctggac gatcccaaca gtgagatttc gcaactgctg cgccagaagc
    35341 ccacttaccg ctacaagctg gcgctgggca ccaaaccgaa gctgtaccgc gtaccgttta
    35401 aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat cgctggtgtg
    35461 ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc tggtgacact
    35521 ggccgtgctg ttacgtcgct tctacccgca ggcaggcggt gcagacagta cgttgctgcg
    35581 caccacgctg attgtcgggc ctggcgcggt gatcctcggt ctgttgatcc tcgtcttcca
    35641 cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta cctcggtgat
    35701 gtcgatgggg gtgatgctgt ttcagcttta catggtggtg ctggtgctgt ggctggcgaa
    35761 aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc tggggatcgt
    35821 gcaaaaggtc ttgagcctgc tgacgcccgt tcatcgcgga ctggaaacat tgatgctggt
    35881 gctggcggtg ttgttggggg cttataccgg ctttctgctg tcggcgctga aatcgtatcc
    35941 gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct cgtccggtgc
    36001 ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt ccacggaagc
    36061 gcactttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc tgctggtggc
    36121 gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg tggcggcatt
    36181 gggcggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg ggctgattgt
    36241 gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg tgctggcggc
    36301 atgtggggcc agcctggtcg gcgtgttgat gctgcgcttt ttcattctct acgccgggca
    36361 gttaacggtg gcgtaagcca gaacagaggt ggtttctgga cgtattcctt cctgaagtcg
    36421 gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gatcccgttg acggccttcg
    36481 cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc attctggcgc
    36541 agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc atcagcgatt
    36601 tctcggttat ttatgtcgcc caacatagct atagcctgct gtcgtgggaa ctcaaactgg
    36661 cggcggtgtg gggcggtcat gaaggttcgc tgttgctttg ggtgctgctg ctttccgcct
    36721 ggagcgcgct gtttgcctgg cattaccggc agcaaaccga tccgctattt ccgctgacgc
    36781 tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtggtgctg tggtccgatc
    36841 ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg atgctgcaac
    36901 atcccggtct tatctttcat ccaccgctgc tttatctcgg ctacggcggt ttgatggtgg
    36961 cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtacc tgcgcccgaa
    37021 tttgctggcg ttgggcacta cctggctgga gcgcattaac agcggggatc atcctcggtt
    37081 cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat ccggtggaaa
    37141 acgcctcttt actaccctgg ctttctgcca cagcgctgct gcacagttta tccctgacac
    37201 gccagcgggg gattttccgc cactggtcgc tgttgctgac gatagttact ctgatgctgt
    37261 cgctgctggg cacgttgatt gtccgttctg gcattctggt ttcggttcat gcgttcgcgc
    37321 tggataacgt ccgcgccgtg ccgttgttcg gcctgtttgt actgattagc cttgcgtctc
    37381 tggctctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt tcggggttat
    37441 cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc
    37501 tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc ctctccgttg
    37561 gcgcgccgta ttttaaccgc gcgacgttac cgtttgctct gttgatgctg gtggtgattg
    37621 tgctggcgac gtttgtctct ggcaaacgcg cgcggcttcc gacgctgctg gcgcatgcgg
    37681 gcgtgctgtt atttgccgcg gggatcgtgg tttccagcgt cagccgtcag gagatcagcc
    37741 tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt tttgagcgcc
    37801 tcgatctgca agccaaaggc aattacacca gcgaaaaagc gatagtggca ctgtttgacc
    37861 atcagcaacg cattggtgaa ttaacgccgg agcggcgttt ttacgaagca cgccgtcagc
    37921 aaatgatgga accctcgatt cgctggaacg gcatccatga ctggtatgcg gtcatgggag
    37981 agaaaaccgg agcggatcgt tacgcatttc gtttgtatgt acaaagcggt gtgcgctgga
    38041 tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg cggggaagga
    38101 agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt tgcgcgcgct
    38161 caggttgtag acacctggca attcgctaat ccgcaacaac aggaacaggc gttaaatatt
    38221 gccagccagt tacgttgtcc gcagtgccag aatcagaact tactggaatc caatgcgccg
    38281 gtggcagtca gtatgcgcca tcaggtttac accatggtgg aagaggggaa aagtgaagtc
    38341 gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa cccaccgtta
    38401 acgggtcaga ctctggtgtt gtgggcgctg ccagtagtgt tgttactgct gatggcactg
    38461 atcctctggc gagtgagggc gaagcgatga aacagcccca aataccggtg aaaatgctga
    38521 caacactcac gattttgatg gtatttctct gtgtcggcag ttatctgtta agtccaaaat
    38581 ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctgcatcag tttgccagcc
    38641 agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgtgct aatccacaaa
    38701 acagcgaaca gtgggcgtta ctgggcgagt attatctgtg gcaaaacgat tacagcaatt
    38761 cgctgctggc gtaccgtcag gcgttgcaac tacgtggtga gaacgctgaa ctgtatgcgg
    38821 cgctggcgac ggtgctgtat taccaggcca gccaacatat gaccgtccag gctcgcgcaa
    38881 tgatcgacaa agccctcgcg ctggactcta acgaaatcac cgctctgatg ctgctggctt
    38941 ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa aaagtgatgg
    39001 atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat atggcgaaat
    39061 tgttgcagcg gagatcagat taatcatctg gtgatgaaga ttatgttatt gagctgc
//
LOCUS       DACADA010000026        38314 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.026, whole genome shotgun sequence.
ACCESSION   DACADA010000026 DACADA010000000
VERSION     DACADA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.026"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            38..580
                     /gene="ssb"
                     /locus_tag="ISB17_001323"
     CDS             38..580
                     /gene="ssb"
                     /locus_tag="ISB17_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1979275.1"
                     /translation="MAARGVNKVILVGHLGQDPEVRYMPNGGAVVNLTLATSETWRDK
                     QTGEMRENTEWHRVVMFGKLAEVAGEYLRKGAQVYIEGQLRTRNWQDDAGVTRYVTEV
                     LVGQNGTLQMLGGRRESGVPESAAQPQNPATPAQPAQAAAKSPKAKGGKKGRQDAAPS
                     QQPPQPLPDDFPPMDDDAPF"
     gene            629..877
                     /locus_tag="ISB17_001324"
     CDS             629..877
                     /locus_tag="ISB17_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1979276.1"
                     /translation="MDHNVLSPLVSLPDGTFTREQAQVVAAQYQNVAIEDDQGTHFRL
                     VVRHQDDGSMIWRVWNFEPGGEDMMNRYIRDYGVRKTK"
     gene            947..3004
                     /locus_tag="ISB17_001325"
     CDS             947..3004
                     /locus_tag="ISB17_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1979277.1"
                     /translation="MSATESKVKTAPKTSKKTLKSAEAEALKVALDAAQVEYVPVTAL
                     VKSPLNVRTIPYPAEKVCSMADSIEAIGLLQNLVVHNLPDGRCGVAAGGRRLKALQLL
                     QSENRIDAGYQVMVKKVPDELAVAASMAENEQQMAMHPSEQIAGFRTLAEQGKTAAQI
                     GDLLGFGTRHVQRMLKLTELAPEILAALAKDEITTEHCQALALESDQKRQVEVLESAR
                     KRSWNNEASVSSIRNLIISEEVSTNGDKFRFVGEAAFSPDEIRVDLFSSENGGYVKSA
                     SLDTALLEKLQNIAEHLREAEGWSWCDGRLDPISHYGKDTKIWRLHAVPPVEYTEAES
                     ERLAELEALEAKYEDENPGVNDDVVAGALEAVWEEQQTIAHRAKHRAWTDEMKQSAGV
                     VVSWTGQEVKVQRGVVLCADEKMEEKDASTDQAPEKVDPLDAVSVPLLTRLSSERTLA
                     VQAALLQQPQKAVALMVWKMCNSVFHTTTSVKEPFCISVSVSHYALTREAPDGENSVA
                     FQAIQSEKERLEALLPENWRKDMTTFFTLDGATLMALMAFCTACSIDGVQGKDESGRK
                     HQSSLDGVENAIQFDLRDWWKPTADNLFSHMKLPHIVQALSQAGLAGAAQDAAKMKKK
                     DAAEHAEHFLSKIRWVPEWMTSADNQKQPAAKSELSLATSQNDTDADAGDVTDHNNPA
                     CAA"
     gene            3049..3480
                     /gene="psiB"
                     /locus_tag="ISB17_001326"
     CDS             3049..3480
                     /gene="psiB"
                     /locus_tag="ISB17_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1979278.1"
                     /translation="MKTLLTLDVLKTMSSDELEDYRAAGEDFRRELSHAVMRDLTSPS
                     GWSVNAEYRCEFGGFFPVQIRFTPSHGHFDVAVCSPGELNPRWIVVFVTRDGQPFSVV
                     RVMDAFNPELITHTLDLIECLDAGGYSFASIISTLSQEGAQ"
     gene            3477..4205
                     /locus_tag="ISB17_001327"
     CDS             3477..4205
                     /locus_tag="ISB17_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1979279.1"
                     /translation="MIPSSSALVSLKPARQAALQAIMTVEEARQRGARLPSMPHVRTF
                     LRLLTGCSRINSDVARRIPGLHRDPKDRLSSLKQVEEALDMLISSHGEYCPLPLTMDV
                     QAELFPEVLHTRTVRRLKRQDFAFTRKMRREARQVEQSWLLRQNLLGQAVTELNFQSP
                     ETVCTWYTRWSDEFDAAELAAPFWRWQSRFTSLKELDWLRISGEPLYAVMYEIPLIVR
                     ETPEHIRVAERWQVPNKLADRSGV"
     gene            4202..4528
                     /locus_tag="ISB17_001328"
     CDS             4202..4528
                     /locus_tag="ISB17_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HAP1979280.1"
                     /translation="MKWRYSLRWKLPHRPCPGPRELISVVVEAGQAAPEEVMSRWVAG
                     SGYAVCVDFHGQKQIQRWSDERKAAVRRRNMQARIHRVAPLFADELIERELAARPEYF
                     NGKSAR"
     gene            4584..4958
                     /locus_tag="ISB17_001329"
     CDS             4584..4958
                     /locus_tag="ISB17_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979281.1"
                     /translation="MRYWLLVILMNVLFWLIVKHVAPEWYGSPCYYAGQIICTLIAAV
                     ITWRDPPDWLDGILLGVIEFGVLIAGIIFFVWGIGYKLAFWGQHDPNTIYASANDVIL
                     PNLALGTGVLIAGWVYIRYLKR"
     gene            5186..5344
                     /locus_tag="ISB17_001330"
     CDS             5186..5344
                     /locus_tag="ISB17_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1979282.1"
                     /translation="MKLPRNTLLGCVLIVCLTLLIFTYLTRKSLCEIRYRDTNREVAA
                     FLAYESAK"
     gene            5413..5577
                     /locus_tag="ISB17_001331"
     CDS             5413..5577
                     /locus_tag="ISB17_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979283.1"
                     /translation="MKGLPFTGSPFFLLTGKKARTTGGQKSFIYLQHRFNESQVTLTS
                     FIHRNTLSSV"
     gene            5654..5989
                     /locus_tag="ISB17_001332"
     CDS             5654..5989
                     /locus_tag="ISB17_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979284.1"
                     /translation="METATAELKDHESVRLSKGSPGKPGATSGCIALNPPACRVFLPE
                     KCCYNLFVQCAVSAFLVNQSPGLPGDTAVHSLYDTVMFACFLSRLFLLIMYDICHTVD
                     CVMNRNCST"
     gene            6037..6309
                     /locus_tag="ISB17_001333"
     CDS             6037..6309
                     /locus_tag="ISB17_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979285.1"
                     /translation="MNFTIKSRKTGEIFSFYAPDSGGYVHLESPGHPGNTGAQICRGG
                     GFMGSTLSCGASEDDLASVARKWYRQFVRERRKFLIMSGQYSEDNQ"
     gene            6306..6656
                     /locus_tag="ISB17_001334"
     CDS             6306..6656
                     /locus_tag="ISB17_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979286.1"
                     /translation="MKKEISRNPSFTPSPKLRAHLNSHREGVTERLNNIFDRYAHLVR
                     ACALPLDDDETQVLLNVLNGSVVEPAFIEYLAQEIRDSDDYLEGIPAAKSLYEKCQSA
                     TYPQLLATVERLDR"
     gene            6671..6988
                     /locus_tag="ISB17_001335"
     CDS             6671..6988
                     /locus_tag="ISB17_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979287.1"
                     /translation="MSDVLSCRQLTANLKMIAGAIGCLNRNDVAQIISLGGVPCSKSR
                     ADSIIRSAGAEKNASGNSHLRGARIKRSADVTPEEFNAFCAGLKTFLVSFETNNVSEN
                     NDK"
     gene            7118..7252
                     /locus_tag="ISB17_001336"
     CDS             7118..7252
                     /locus_tag="ISB17_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979288.1"
                     /translation="MLIVLDSSIGSARYFSAGFCSYIPPFLPVMALFPAVSLFKTEKD
                     "
     gene            7396..7572
                     /locus_tag="ISB17_001337"
     CDS             7396..7572
                     /locus_tag="ISB17_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979289.1"
                     /translation="MSEVRVNGSACCDRSGRAAFTVPAIKLPALCRATPFRARALRRC
                     GGCLLLSPGAVSPV"
     gene            7708..7824
                     /locus_tag="ISB17_001338"
                     /pseudo
     CDS             7708..7824
                     /locus_tag="ISB17_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            7829..8185
                     /locus_tag="ISB17_001339"
     CDS             7829..8185
                     /locus_tag="ISB17_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979290.1"
                     /translation="MSTQNVNVKPSTKESTTLRTDGFVRNIHSRNPFDVIRADVVLER
                     IEKEAGWCCGMHYELYQAHLLGGALDYLDALPLKDRPVLMGAAAKRGYLLTLAEEERA
                     LEARDVLMSELAANDC"
     gene            8246..8458
                     /locus_tag="ISB17_001340"
     CDS             8246..8458
                     /locus_tag="ISB17_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979291.1"
                     /translation="MKVYGHQHSSEVFSRETSPSCFEPKAEKLIAFHGKDASYAYNVL
                     ADLKAAGASYTRRNIIADMFRRLGVE"
     gene            8469..8693
                     /locus_tag="ISB17_001341"
     CDS             8469..8693
                     /locus_tag="ISB17_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979292.1"
                     /translation="MEHVKRQIQQGAGNAYYAGMQWRGITLGHADAAALNQFDIDISK
                     AQAPEARTWLLQNKAEFIALMLGIEIVKIG"
     gene            8774..9094
                     /locus_tag="ISB17_001342"
     CDS             8774..9094
                     /locus_tag="ISB17_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1979293.1"
                     /translation="MFTVIFHDEAEKEFTALPAAIRAKMARLLMKLEANPRQLREPDT
                     KPLGNGLFEIRTMGADIARGIWVYQSGERIFLLRIFIKKSPKTPPAEIDLALRRLEEM
                     QNEI"
     gene            9084..9362
                     /locus_tag="ISB17_001343"
     CDS             9084..9362
                     /locus_tag="ISB17_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1979294.1"
                     /translation="MKYKTLSQVHTEAMNDHEYSAAFEAEEASELLRETLATWRKEAG
                     LTSAQVAERMGIKAPTISRMEKNASRMSIQTLVRYARACGVNFKIQQV"
     gene            complement(9366..9581)
                     /locus_tag="ISB17_001344"
     CDS             complement(9366..9581)
                     /locus_tag="ISB17_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979295.1"
                     /translation="MRAARQRGPVFPAPQARGLSRRGQTGTDAPFACSVPESVNKHRD
                     RDLKSICQRPHVIEGQTAFTTEDPADE"
     gene            9847..10188
                     /locus_tag="ISB17_001345"
     CDS             9847..10188
                     /locus_tag="ISB17_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979296.1"
                     /translation="MLRPSRSRRFHRAGEGLPALCRATPFRARALRRCGGCLLLSPGT
                     RITRLSPFAGAETSTTRLAVQGTAEPFAINFSRPFRCASLDRKKFPAHPRTSAPGRAR
                     LRPRGERLSNG"
     gene            10185..10271
                     /locus_tag="ISB17_001346"
     CDS             10185..10271
                     /locus_tag="ISB17_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979297.1"
                     /translation="MKVISRGQVQQVPAQSQNRGPGNSTGDG"
     gene            10299..11120
                     /locus_tag="ISB17_001347"
     CDS             10299..11120
                     /locus_tag="ISB17_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1979298.1"
                     /translation="MRLASRFGLTHSIRQERPLTNDELVKVVPSVFSEEKHNSRSDRY
                     TYIPTITLLDKLREEGFQPFFACQSRVRDEDKRGHTKHMVRLRREGANKGTEVPEIIL
                     LNSHDGSSSYQMIPGMFRFVCTNGLVCGTSFGEIRVPHKGDIVGRVIEGAYEVLGIFD
                     KITEGVDVMKSIALTKEEQRLFGQAALTYRYEDENKSPVSIEQIIHPRRYEDKKDDIW
                     TTYQRVQENLIKGGLPGRTEKGKRTTTRPVKAIDGDVKLNKALWLIAEKFRTLKG"
     gene            11153..11482
                     /locus_tag="ISB17_001348"
     CDS             11153..11482
                     /locus_tag="ISB17_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979299.1"
                     /translation="MVGILMFEFKAVCISISHMEEGDIRVLQVLSSTTNYLTCDSYLE
                     WIHDTPYGCVIRMYDFKRHLLALKRGGVSKFLRKTLILMHREGGYLLFHFDSDADYYP
                     GIDAVDF"
     gene            complement(11515..12000)
                     /locus_tag="ISB17_001349"
     CDS             complement(11515..12000)
                     /locus_tag="ISB17_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1979300.1"
                     /translation="MKGVAFGIILSLIPLSSMAADCFDMAGRDYKIDPDLLRAISWQE
                     SGFKIDAIGRNPVTGYGSGLMQIDSQHFNELSRYGIKPDHLLSDACLNIYTGAYYLAQ
                     AFKKWGVSWDAVGAYNAGFKKTPRQAARRYEYAKKIHYYYTAIKASKRTSSKDQKIAM
                     N"
     gene            12391..12807
                     /gene="traM"
                     /locus_tag="ISB17_001350"
     CDS             12391..12807
                     /gene="traM"
                     /locus_tag="ISB17_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAP1979301.1"
                     /translation="MFDAVFNKGTDMARVQAYASDEVAEKINAIVEKRRAEGAKEKDV
                     SFSSVASMLLELGLRVYEAQMERKDSGFNQTEFNKILLENVMKTQFTVSKLLAIGSLS
                     PHVTGDERFEFRSMVSNIREDAKDVISHFFPEQEEE"
     gene            13007..13744
                     /locus_tag="ISB17_001351"
     CDS             13007..13744
                     /locus_tag="ISB17_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HAP1979302.1"
                     /translation="MCAKDRSHIAVRSQTDSIHSMREMLNISSCPAFIRDSRGEVIHT
                     SPTFDKIFLTSGGTGAFDTPRAPGSWFLDLSLEIKLELMQSELKSFSEGSAVLVKNIW
                     LAGSLWTVFIETFSINEDTYSKWVFINEDEPIPHPSMEYNDFSIKMQRYIERIQRSAK
                     SDWAIFNLYAVGFSHASISKITGVGVQTSKNTVSKIKKELSFDDRDYIIMSSIYTLSY
                     GKFISNVVSILKDGVNFLLNQKYHRYF"
     gene            13895..14065
                     /locus_tag="ISB17_001352"
     CDS             13895..14065
                     /locus_tag="ISB17_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HAP1979303.1"
                     /translation="MLLKLDEETNRRLIKAKDRSRRSKTAEAYLRLKDHLERFPDFYN
                     SELAVPGGEKEE"
     gene            14127..14495
                     /gene="traA"
                     /locus_tag="ISB17_001353"
     CDS             14127..14495
                     /gene="traA"
                     /locus_tag="ISB17_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAP1979304.1"
                     /translation="MNAVLSVQGSAVSEKTKPSFMSRVFNKKKALKVAKVALPVAAVA
                     AFFPDAAMATTTATDLMKSGDATVKGTFGKQSSVVKWVVLAEVVAGGIMYMMTKNVKF
                     LFGFAIISTFITIGMSVAGY"
     gene            14509..14814
                     /gene="traL"
                     /locus_tag="ISB17_001354"
     CDS             14509..14814
                     /gene="traL"
                     /locus_tag="ISB17_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAP1979305.1"
                     /translation="MTGDELKRYRFPETLTNQSRWFGLYLDELIPAVICLGWGFWTSK
                     FIFGIASAVLVFWGIKKLKKGRGSSWLRDLIYWYLPTSLLKGFFHDVPDSCFRQWIK"
     gene            14834..15400
                     /gene="traE"
                     /locus_tag="ISB17_001355"
     CDS             14834..15400
                     /gene="traE"
                     /locus_tag="ISB17_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAP1979306.1"
                     /translation="MEHGARQNTTRVIAVGFISLGTLLTLSLATNIIQGINNYRLQTE
                     QKVAVTPMLFRAPFAVSQNQADASYIEQLGLSFVALRLNVTPETVDAQHQQLLRYVLP
                     ASQNSLKVQLAEDAKRIKDNNVNSTFYMTSMRAWPAENRVDIRGELKTWIGDSKPYSE
                     IKSYVIQFSRVDGVSWLARFGEINNEKN"
     gene            15387..16127
                     /gene="traK"
                     /locus_tag="ISB17_001356"
     CDS             15387..16127
                     /gene="traK"
                     /locus_tag="ISB17_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAP1979307.1"
                     /translation="MKKINPLFISGCLLLAAPAMSATLSGTLAPTVVPLTNGGQANIA
                     VSNTDPNLFTVPGDRITAINSLDGGLTNQEQTDSGGAILATVSKKPFTFIVETERGLN
                     FSIRAVPRAGSGRTIQLVSELAGTPGPAKAWEESNPYESLLVSLNRAVRQGSVPDEYQ
                     SVPVTSEVLQVPAGLRATADRVWVGHHLKVVRYSLDNVSLSARMVRESDFWQPGTRAV
                     MFSTPAGLLTAGGRMQIWVTTSDEGVKR"
     gene            16127..17551
                     /locus_tag="ISB17_001357"
     CDS             16127..17551
                     /locus_tag="ISB17_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraB"
                     /protein_id="HAP1979308.1"
                     /translation="MANFNTLIKRKQYIWLGLILAGGAAAVGGGLYLSDLNMSSDEEA
                     PAQGEPAPDMTGVVDSSFNSKVEQHATTEMQATAADLNKRFDSLQGEVELLSKARTAD
                     QIRIDKLSSDNEAMQNQLKSLGVKPAVSGGEPAPTPPSPPPGPEGEPQPASYPPQTGA
                     AVPPPTAFYPGNGMTPPPQVSYQSVPVPNQIQRKTFSYDKGKKTKSLPYIPSGSFAKS
                     MLIEGADANASVTGNESTVPMQLRITGRVEMPNSKTYDLTGCFVGLEAWGDVSSERAI
                     VRTRNISCIKGDKTIDQPINGHVSFMGKNGVKGEVVMRNGKILGWAWGAGFVDGIGQG
                     MERASQPAVGLGATASVGAGDVLKMGVGGGASKAAQTLSEYYIKRAEQYHPVIPIGAG
                     NEVTVVFQDGFQLKTIEELEAEKNGQQQAQNQEAEPAQQADAHHQSGGGMNGFNTDEM
                     LKKLGKLDPREFSPGAAPQGENNG"
     gene            17623..18207
                     /gene="traV"
                     /locus_tag="ISB17_001358"
     CDS             17623..18207
                     /gene="traV"
                     /locus_tag="ISB17_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAP1979309.1"
                     /translation="MKELMLLIPLGSALLLTGCAGTETEFQCNATTSDTCMTMEQANE
                     KAKLKEERSDAKPAAAGLPQLAEGNFGTTSVNPYPLPPQPNLNRARVAAERERARAVY
                     LNNPTAKNEAIYFETGKRLQSLPVVKMSLPVVASTFTSPSTPPGNYPRPLRKGEETTS
                     LWIAPYVDSDDVYHQPATVLFVVKPSAWGQPRLN"
     gene            complement(18339..18464)
                     /locus_tag="ISB17_001359"
     CDS             complement(18339..18464)
                     /locus_tag="ISB17_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979310.1"
                     /translation="MPIATYRSVRIRLAYIFNNITTLKSILPGEKNPLSSTRPFK"
     gene            18530..18748
                     /locus_tag="ISB17_001360"
     CDS             18530..18748
                     /locus_tag="ISB17_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979311.1"
                     /translation="MHAFIFLVLMTPSSGGGIWDITPMPNTDVCIKMLRVLEARGGRG
                     YGSNTGECIEIKTREPLSSVIETLNEGG"
     gene            18749..19060
                     /locus_tag="ISB17_001361"
     CDS             18749..19060
                     /locus_tag="ISB17_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979312.1"
                     /translation="MAEYQLGFDGFVRLFFAMAIIICGYGYVIIGVFIRDTLSCILML
                     FGLTGLSALFSLMVFMDKNMWAGSPDLTLSVITGFFTATVVFYMFYVLIYYFDRMGAG
                     E"
     gene            19127..19531
                     /locus_tag="ISB17_001362"
     CDS             19127..19531
                     /locus_tag="ISB17_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979313.1"
                     /translation="MKAYYILGHNVAWLNGICLILFVIGVVGALAMVAIPEKFNLRVN
                     RGDTFIYCALIAVVGFSGMFVISIHSFSMDELEAGRHWKDDCRTLEVNMPTGAFTSPV
                     NKLDCDGIIINVPGGQYYSYIHQWELYKANSK"
     gene            complement(19518..19724)
                     /locus_tag="ISB17_001363"
     CDS             complement(19518..19724)
                     /locus_tag="ISB17_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979314.1"
                     /translation="MNNTLQLFWSSGLHIARIFNILHKKNTQLTQLKEELSKNQIAGQ
                     FLAVFPLFHPYRSIQFVNQHFILS"
     gene            19908..20306
                     /locus_tag="ISB17_001364"
     CDS             19908..20306
                     /locus_tag="ISB17_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979315.1"
                     /translation="MDMMLFLRKYGFLFIPPVYWLIYLGARLTYNGLAGEFSPDFFQG
                     IRHEGVYIICSLCVFYFFCLEVEVGDGEFSANILFLIVFFHVVLLFITLLICLWQGEV
                     LLDSIFMGQIIASMCLLFFIPVLSIIRRSF"
     gene            20378..23017
                     /gene="traC"
                     /locus_tag="ISB17_001365"
     CDS             20378..23017
                     /gene="traC"
                     /locus_tag="ISB17_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAP1979316.1"
                     /translation="MSNNIIDAVTQTVNSLVSALKLPDESAKANDTLGSMNFPQFSRI
                     LPYKDYDSATGLFINNKTIGFMFEARPLPGADKSIVATLEHLLRSKLPRGVPVSFHLV
                     SSKLVGNDIDYGLREFRWSGKQAKKFNAITQAYYLRAAETKFPLPPALDLPLTLRNYR
                     VYISCCVPRKKNSTTQIVEMENQIKILRASLGGAYIPTRILDAAGLVELMRELINPDP
                     HEMYRVPYKLDPYQDLNYQCVDDSFDMQVTAGHLKIGRLGRDGKECVTRVTSYHLESD
                     PEMAFLWTSADNYANLLNPELSISCPFVITLTLMVEDQVKTQNEANMKFMDVEKKSKT
                     SYAKFFPNVIKEMQEWGDIRQRLATNQTSLVSYFFNITTYTADSTEASLAAEQQVLNS
                     YRKGGFQLIPARYHHLRNFLAMMPFKCGEGLFKELQAAGVVKRAETFQVANLLPIVAD
                     SPLAPAGLLAPTYRNQLAFIDLFYEGMNNTNFNMAVCGTSGAGKTGLIQPLIRSVLDS
                     GGFAWVFDMGDGYKSLCENMGGVYLDGDTLKFNPFANVLDDAHFDMSAERIRDQMSVM
                     ASPNGNLDEVHEGLLLQAVQAAWLSKRNHARVDDVVQFLQDAKDSDEYADSPTIRGRL
                     DEMIILLDQYTVNGIYGDYFNSDKPTLHDDARMVVLELGGLESRPSLLIAVMFSLIIY
                     IENRMYQSPRGLKKLNVIDEGWKLLDFKNEKVGQFIEKGYRTARRHTGAYITITQNIV
                     DFDSPTASSAARAAWGNSSYKAILKQSAKEFAKYNQLYPDQFSKLEKDMINGFGSAKE
                     QWFSSFMLQVEANCSWHRLFVDPLSRAMYSSKGPDFEYMQARRQEGVDIHDAVYGLAC
                     RNFKDEMAELESRIPVNDMEDKQ"
     gene            23017..23406
                     /gene="trbI"
                     /locus_tag="ISB17_001366"
     CDS             23017..23406
                     /gene="trbI"
                     /locus_tag="ISB17_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAP1979317.1"
                     /translation="MKNNTEQPVSTTSLTFRRIPVIVALVLFALATSAFLSRVVLDYS
                     TPRVVAFDMKKTLDSFMDSVSQKQLTEAQSKALSDRFNDALEKSLAEYQQQHHVVILV
                     SPAVVQGAPDVTRNIQHDIARRMKGEE"
     gene            23406..24041
                     /gene="traW"
                     /locus_tag="ISB17_001367"
     CDS             23406..24041
                     /gene="traW"
                     /locus_tag="ISB17_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAP1979318.1"
                     /translation="MIRLKDCLLAACLLSPLTQAADLGTWGDLWPVREQDMLQLITQR
                     LQSLQSSGQWGQTMDAFKQRVIENSQRPAPVEGIKRAEKYEQRWFDPSIRLTEDLKDN
                     EGRVFARKGEVVNPLKTVPFVQTLYFINGDDADQLAWMKRQVPETLMSKIILVRGSIP
                     DTSAALDSRIYFDQNGVLSKRFGLTAVPARITPAPSGERLNIETFPPVPHP"
     gene            24076..24477
                     /locus_tag="ISB17_001368"
     CDS             24076..24477
                     /locus_tag="ISB17_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979319.1"
                     /translation="MPMILALIILLTMLLLTLMWSTIRKQENVIRELSTRESWLEREV
                     ERLRTDNEKLYRWLRYNGEKVIDEMTCELDALSAVRQTNRDSYRRQLYLFDCLSLMVW
                     AGMEGAEDSTREKVRNKTDALRLVLREKTDE"
     gene            24474..25463
                     /gene="traU"
                     /locus_tag="ISB17_001369"
     CDS             24474..25463
                     /gene="traU"
                     /locus_tag="ISB17_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAP1979320.1"
                     /translation="MKAIFFLICIAFSTFSGRAAADPSCEGRFVNPITDVCWRCIFPL
                     SLGSVQVGKGDLPDTSNPGSPLQLCPAPPPIFVRPGLAIGYWEPMAMTDVSRSPGCMV
                     NLGGFSINLGKTGMGTARKDDKQVNGAFYHVHWYKYPLTYWLNIITSAGCLEGGDMDI
                     AYLSEIDPTWVDSSLTTILNPEAILFANPIAQGACAADAMASAFHMPLDILFWCAGSQ
                     GSMYPFSGWVSNESSPLQSSLLVSERMAYKLHRQGQIMESIGKDKAVCYEYPSPIIPK
                     ERWRYQMVNMYPDSGQCHPVGRSVMRWEAGKNPPNTRKNYGYLMWRKRNCVFL"
     gene            25476..26114
                     /gene="trbC"
                     /locus_tag="ISB17_001370"
     CDS             25476..26114
                     /gene="trbC"
                     /locus_tag="ISB17_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAP1979321.1"
                     /translation="MKLMNLFMVMLLLTGRAVLAETSSVTDREWLKQQENLSEQLRQH
                     PDKQLQQELEAQINRNPLPKSDRQFIDNLVSQQKAANQEKPAEGALYFVSFSIPEEGL
                     KRMLHETRQYGIPATLRGLVNNDMKTTTDAVLQLVKDGVTDGVQIDPTLYTQYSIRSV
                     PSLVVRCQAGYDVVRGNIHVKQALEKVAQTGDCAQVARQILDAPSNAAGSQP"
     gene            26173..28128
                     /gene="traN"
                     /locus_tag="ISB17_001371"
     CDS             26173..28128
                     /gene="traN"
                     /locus_tag="ISB17_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAP1979322.1"
                     /translation="MKTVISVLTAHFFVLSAFIWLASPACADSGSDYKAGSDFAKQVQ
                     GNGLNSLKNFSGEQNLPGYTDNPDQTKYYGGVTASGDSSLKSDSALEFSQGDTGKTIT
                     ESFTNRPPDQISQDAPFIQAAKDTESRADSIVGDTGQSCTAQVVNRSEFTNHTCERDL
                     QVENFCTREATLKDNATTQKVNRTYQQVVTLNYARSTRQWSGNLTIPTNGRLLNASVD
                     GEPLVIPWIEECDSEGKVRDSCKSAVSESLTLFERTFPIDVISWPRSESMCSGGQNTH
                     CTKYTYDGKGKIHQSFGVDKAVIAGQNFSVSKTSRTVSSASQKPVQVTVTLVMEETET
                     VYAPEVVWVESCPFSKDEGKKTGEECISPGGTRTITLGGRDYSFTEACWKYKDTWLTQ
                     PADNGSCESLMKNTACTLSSRQCAFSSEEGTCLHEYATYSCETRTSGKQMICGGDVFC
                     LDGECDKATSGKSNDFGQAVSELAALAAAGKDVAALNGVDVRAFTGKAKFCKKFAAGF
                     SNCCKDSGWGQDVGLASCSSEEKALAKAKKDKLTVSIGEFCSKKVLGICLEKKRSYCQ
                     FDSKLAQIVQQQGRNGQLHIGFGGASSPDCRGITVAELQGIDFNKLDFTNFMDDLMKN
                     QKIPENDVLTNKTRERIKEIMSQQSAQ"
     gene            28160..28414
                     /gene="trbE"
                     /locus_tag="ISB17_001372"
     CDS             28160..28414
                     /gene="trbE"
                     /locus_tag="ISB17_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAP1979323.1"
                     /translation="MKMSFHKKPVIDFVLRLTLTIIIISPVIYFSWDAVKGTSGSDYV
                     ESVVFILMAGFAMSVCYLFFSVLEKVINARKSKDESIYHD"
     gene            28392..28640
                     /locus_tag="ISB17_001373"
     CDS             28392..28640
                     /locus_tag="ISB17_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979324.1"
                     /translation="MNQYITIEKFIDILNEENLPQEHHVMVLAVLADISLHTDRFLIN
                     SSELVQMAAQYSPAFQKLPADRQAFISSVLSMPLFLIM"
     gene            28653..28979
                     /locus_tag="ISB17_001374"
     CDS             28653..28979
                     /locus_tag="ISB17_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979325.1"
                     /translation="MEEKKAYGLVMVFVGVFVFLLVSIMSYSLWRDRQVNAFMTTNRA
                     WGIQCDTVSQAAWVIRDGERVDLQINYLPLYCSGYRFEARDDAGKVQRQLDKYSVYQH
                     LSRQSH"
     gene            29000..29752
                     /gene="traF"
                     /locus_tag="ISB17_001375"
     CDS             29000..29752
                     /gene="traF"
                     /locus_tag="ISB17_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAP1979326.1"
                     /translation="MRTIHAAMLAGCLCCALMPAFAKDAGWQWYNEKLKPRDEDRKAV
                     PAAPPPQMDILEKLATLQAATKRSLYEAILYPSSENFVKYFRLQNYWTQQAGLFSMSA
                     KKAMLENPELDYNLQYSHYNGTVKNQLAADYAEQRQAISTLAQHYGVMFFYRGREAID
                     GQLVQVIKNFRETYGLSVIPVSVDGVINPMLPDSRTDQGQAEQLGVKFFPAMMLVNPK
                     SGQVKPLSYGFISQDDLAKQFLYVSSDFKPNF"
     gene            29763..30002
                     /gene="traQ"
                     /locus_tag="ISB17_001376"
     CDS             29763..30002
                     /gene="traQ"
                     /locus_tag="ISB17_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAP1979327.1"
                     /translation="MRNIRFPDLDITGMWVLAVGVFFHLIARLVRKQPELAVQAGEIF
                     GLGMVVFGGYRILNALIAEAEKEEKACEKQPDEPR"
     gene            29974..30531
                     /gene="trbB"
                     /locus_tag="ISB17_001377"
     CDS             29974..30531
                     /gene="trbB"
                     /locus_tag="ISB17_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAP1979328.1"
                     /translation="MKNSLMNLAKTPLAALALSLALAGVAHAGTLDEVKSLWDPRGIS
                     GTETASPQSDTPQAVSGASQPRWLRLSNGKQVDLRDWKVVLFMQGHCPYCHKFDPVLK
                     QLAGQYGFSVFSYTIDGQGDDAFPEALPAPPDVMQTFFPNIPVATPTTFLVNVNTLAA
                     YPILQGATDAQGFMARVDTVFQMMH"
     gene            30577..31020
                     /gene="trbF"
                     /locus_tag="ISB17_001378"
     CDS             30577..31020
                     /gene="trbF"
                     /locus_tag="ISB17_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAP1979329.1"
                     /translation="MNKYIRSTGLYAFLFPASLKDSGQTAAERIEQLKPEFIHRERRL
                     EIYLELFIVFLTAGALLLWIMRFLFNLCVDDWIASGDLRVKDLWNIMMYAIPYALIAV
                     GVGFFVAGVTLAIRNFFSYHLKTLFILRNDRVKKNAVRNGGQDAN"
     gene            30998..32377
                     /locus_tag="ISB17_001379"
     CDS             30998..32377
                     /locus_tag="ISB17_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HAP1979330.1"
                     /translation="MEDRMRIKLLSLFCGAALGLSFSAAADVNGDMNNFFNKLGFASN
                     TSQPQVWQGQAAGYASGGSLYARTQVKTIQLVSMTLPDINAGCGGIDAYLGSFSFINS
                     EQLQRFGKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMATDMNSMNLSSCQAAQGI
                     VGGLFPRTQVAQQKVCQDIAGESNIFSDWAASRQGCTVGGKMDSVQDKASDKDKERVM
                     KNINIMWNALSKNRLFDGNKELKEFVMTLTGTLIFGENSEITPLPARTTDQDLIKAMM
                     EGGTAKIYHCNDSDKCLKVVADATVTITANKALKNQIRALLSSIQNKAVADEKLTEQE
                     KGFISSTTIPVFKYLVDPQMLGVSNSLIYQLTDYIGYDILLQYIQELIQQARAMISTG
                     NYPQSTMDMIMENLNQASVQIAAFQSRVQVQQDALLVVDRQMSYMRQQVSARMMTRYQ
                     NNYHFGGNL"
     gene            32377..35226
                     /gene="traG"
                     /locus_tag="ISB17_001380"
     CDS             32377..35226
                     /gene="traG"
                     /locus_tag="ISB17_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAP1979331.1"
                     /translation="MLEIYAIAGGDWLRGNLNAIAAFMGTSTWSTIEKMCIAISVLIV
                     AGNWVKKHNVMDLIGWVFSLTLVSMLVVIRTPVQIIDYSNVAQVYEVDNVPIGLAIPA
                     SLTTRVGNALIQSYEMVFALPDSVTYSKTGMLFGSNLVAKSTDFLSQNPQITTLFSDY
                     VQNCVMGDIFLNHKYSFEELLNSPDPYTLIFANPSPLRGVFDKNNQFQTCEEASRDLK
                     SALALDTQTGGKTWNYYVRQLFGGKPNPDLLFSQMIGDSYNYFYSSGQSAGQIIRQNV
                     TMNALRSGIQSYAARSGDTASLVNMANTSSLEKQRLAQATMGHQALRALPLMQTVIMG
                     LMIGMFPIMVMAAMFNMMTLQVLKGYVFALIWLQTWPLLFAILNSAMAYYAKQNGVPV
                     VLSELSQVQLKNSDIATTAGYIAVMIPPLSWGIVKSMGAAFSSAYSHFSSSGLSATSQ
                     AASGVVDGNYSFANMQMENVSGYSWGTNSTTSFGQMSRQLANGGMSTQTRDGSMVWDS
                     GGAMSKLPVDINVGRQIASAQQQMAREADVQAESALHGYNSSVTSAWNSLQQFGTNKG
                     NSTSTTTGADTTESSQDSMARSKMWNAVVANAKANNISNEESFQQLMDDSSKRTQSFD
                     AHVSGKWSSGDQLFGKLGKWGSGFSAEVGGKGSAGWGHSSGNTDNVGTSGRESNDSRH
                     DTSSQAAKDFKEASDYFTSRKTTTSGNITDNNASSRVDQFAASLSSAKNSYDQYTSSR
                     TRSHEYSEMASRTESMTGQMNENLTQQFANFVQHRAPQDAEAILTNTSSPEIAAQREA
                     LAREFVKEQVEPRVDAAYQQGRESIGQNMAGVSGGGDNGSVMADYGQNSRRIDVMTQD
                     AGIKDNVGQKVDRMITENKQQHQETRENIQRQGAEVKSENAEMEKNHKTKANEFKGDY
                     NERKSKVNSLPGADSQAELEEKAAKTQKDFLDGKR"
     gene            35229..35762
                     /locus_tag="ISB17_001381"
     CDS             35229..35762
                     /locus_tag="ISB17_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HAP1979332.1"
                     /translation="MMMTNKILIEEVDELKNVFSRGEMEIPSFWSCLWPGVIVAAWNM
                     ICALISVGGGYLSVKFIFWVLVFPGVISLIVLLGVASARSLFLSVPRTYRMRSQVYRF
                     ISRKVMVYALVYMALILALALFNRVYYASPFPFSFMILIATIFVWFVMNIDFGRYQLS
                     VLSSAINSFRAEKLGGG"
     gene            35948..36679
                     /gene="traT"
                     /locus_tag="ISB17_001382"
     CDS             35948..36679
                     /gene="traT"
                     /locus_tag="ISB17_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAP1979333.1"
                     /translation="MKLNKFMAVMVVSSALVLSGCSAMGTAIKKRNLEVKTQMSETIW
                     LEPSNNKTVYLQIKNTSDKDMSGLQAKIASAVTSKGYQVVSNPDTAGYWIQANVLKAD
                     KMDLRESQGWLSRGYEGAVTGAALGAGITAYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKTQVQTDNVAVLRQGTSGAKVQTSTETGNQHKYQTRVVSNAN
                     KVNLKFPEAQPVLEDQLAKSIANIL"
     gene            36872..37561
                     /locus_tag="ISB17_001383"
     CDS             36872..37561
                     /locus_tag="ISB17_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979334.1"
                     /translation="MTISVPVRSLDTHELMPGDIYPHPAAGGIDSLSHREVCSLFSDH
                     IMYICSALVILLLVFIAGVFFRKIMTSSVERKRNTASVISDAKPEVQSKPEYFHLSIC
                     VEGPASTDDSALSSLASRVVREAENLYIPGVNRRDSADDVFAWSVQTQFPSDRLFGTR
                     IFPSTGWFERPDMIKHPVKCYCVCLQYTTETLPSTLYALVDEAGRQVAKSYIKGGHCP
                     GSEGYTFCITS"
     gene            37688..>38314
                     /locus_tag="ISB17_001384"
     CDS             37688..>38314
                     /locus_tag="ISB17_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HAP1979335.1"
                     /translation="MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLM
                     LMYRLSWQTFVNGCVYWWCTTLGPMRDIIRSQPVYTIQYYGQSLEYTSEQILADKYTI
                     WCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQQSEDENTGGRQLSDKPKEVA
                     RQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYV"
BASE COUNT         9605 a         9236 c        10300 g         9173 t
ORIGIN      
        1 ttttaaaagg caaaacaacg gattaaggag attcatcatg gcagcacgtg gcgttaacaa
       61 agtcattctg gttggacatc tgggacagga cccggaagtg cgttacatgc ccaacggggg
      121 cgcagtggtc aatctgacgc tggccacgtc tgaaacatgg cgtgacaagc agacgggaga
      181 gatgcgcgaa aacaccgagt ggcaccgtgt tgtcatgttc ggcaagctcg cggaagtggc
      241 gggtgaatac ctgcgtaaag gcgcacaggt ctacatcgag ggccagctgc gcacccgcaa
      301 ctggcaggac gatgccggag tcacgcgcta cgtcaccgag gtgctggtcg ggcagaacgg
      361 caccctgcag atgctcggcg gccgccgtga atccggagtg cctgagagcg ccgcgcagcc
      421 gcaaaatcct gccaccccgg cacaacctgc tcaggccgca gctaaatccc cgaaagcgaa
      481 gggcggtaag aaaggccgtc aggatgccgc cccgtctcag caaccgcctc agccactccc
      541 ggatgatttc ccaccaatgg atgatgacgc gcccttttaa accacgatga agacgccccg
      601 ttcaggcggg gcagaaaagg agaaacacat ggaccataac gttttgtccc cgcttgtatc
      661 cctgccggat ggcacgttta cccgcgagca ggctcaggtt gtcgcagcgc agtatcagaa
      721 tgtggcgatt gaagatgacc aggggaccca ttttcgcctt gtcgtccgtc atcaggatga
      781 cggcagcatg atctggcgcg tctggaattt tgaaccgggc ggcgaggaca tgatgaaccg
      841 ctacatccgg gactacggag tacgcaaaac gaagtaagca gtaagaagaa ggccggacag
      901 gtgggctgtc cggcagacat tcatcaatag ctaagaggta tcaattatgt ctgcaacaga
      961 gtctaaggta aaaactgcac cgaaaaccag caaaaaaacg cttaagtctg ccgaagctga
     1021 ggcgcttaaa gtggctctcg atgccgcaca ggtcgagtat gtcccggtga cggcactggt
     1081 gaagtctccc ctgaacgtca ggaccattcc ctatccggcg gagaaagtct gttcaatggc
     1141 agattccatt gaagccattg gcctgttgca gaatctggtc gtgcataacc tcccggatgg
     1201 tcgctgtggt gtggctgccg gtggtcgtcg cctgaaagcg ctgcagctcc tgcagagtga
     1261 aaaccgtatc gatgccggat atcaggtgat ggtgaaaaag gtacctgatg aactggctgt
     1321 tgcggcatca atggcagaaa acgaacagca gatggcaatg catccgtctg aacagatcgc
     1381 cgggttccgc acactggcag agcaggggaa aaccgccgcg cagattggcg atttgcttgg
     1441 gttcggtacg cgtcatgttc agcgcatgtt gaagctgacc gaactggctc ccgaaattct
     1501 ggcggcgctg gcgaaggatg aaatcaccac cgaacactgc caggcactgg cgctcgaaag
     1561 cgatcagaaa cgccaggtgg aagtgctgga atccgcccgc aaacggtcct ggaataatga
     1621 agcatcagtt tcctcgatcc gtaacctcat tatctcagaa gaggtttcca caaacggcga
     1681 caaattccgc tttgtgggag aggccgcatt cagtccggat gaaatccggg ttgatctctt
     1741 cagcagcgaa aacggcggct atgtcaaaag tgcatcgctc gataccgccc tgctggaaaa
     1801 actgcagaac atcgcggagc acctccgcga agcggaaggc tggtcatggt gtgatggtcg
     1861 tcttgacccg atttcacact acgggaagga cactaaaatc tggcgcctgc atgctgtccc
     1921 gcctgtggaa tataccgaag ccgaatcaga acgtcttgca gagctggaag ctctggaagc
     1981 taagtatgaa gacgagaacc ccggcgtgaa tgatgacgtc gtggccgggg ctctggaagc
     2041 cgtctgggaa gaacagcaga ccatcgcgca ccgggcaaaa caccgggcat ggacggatga
     2101 aatgaagcaa tctgccggag tggtggtttc ctggactggc caggaggtga aagtacagcg
     2161 cggtgtggtg ctgtgcgccg atgaaaaaat ggaggagaag gatgcatcca ccgatcaggc
     2221 ccctgaaaaa gtcgatccgc tggacgccgt cagtgtcccg ttgctgaccc gcctttcttc
     2281 tgaacgcaca ctggcggttc aggcggccct gttgcagcag ccgcagaaag ccgtcgcgct
     2341 gatggtctgg aaaatgtgta actcggtttt ccataccaca acgtccgtca aagagccgtt
     2401 ctgcattagc gtgagtgtgt cgcattacgc gctgacccgt gaagcacctg acggtgaaaa
     2461 cagcgttgcg tttcaggcca tccagtcaga aaaagaacgt cttgaagccc tgctgccgga
     2521 aaactggcga aaggatatga ccaccttctt tacgctcgat ggcgcaaccc tgatggcact
     2581 gatggctttc tgcaccgcct gctccataga tggtgtacag ggcaaggatg aatctggtcg
     2641 taaacaccag agttcgctgg atggggtgga aaacgccatt cagtttgacc ttcgtgactg
     2701 gtggaaaccg acggctgata accttttctc gcacatgaag ctgccacaca ttgtacaggc
     2761 actctctcag gccggactgg ctggcgcagc acaggacgcc gcgaagatga agaaaaagga
     2821 tgccgctgaa catgcagagc acttcctctc aaaaatccgt tgggttccgg aatggatgac
     2881 cagtgctgac aatcagaaac agcctgccgc aaagtctgaa ctcagtctgg caacaagtca
     2941 gaacgatacc gacgctgatg ccggcgacgt caccgaccat aacaacccgg cctgcgccgc
     3001 ctgataacgg agacgccgcc ctgcggggcg gcgataagga gactgaaaat gaaaacgtta
     3061 ctgactctgg atgtcctgaa aaccatgtct tctgatgagc tggaagacta ccgcgccgcc
     3121 ggtgaagact ttcgccggga gctgagtcac gctgtcatgc gcgatctgac ctcaccgtcc
     3181 ggctggtccg tgaatgccga ataccggtgc gaatttggcg gcttctttcc tgtccagatc
     3241 cgttttaccc cctctcatgg tcactttgat gtggcggtat gcagtccggg ggagttgaat
     3301 cctcgctgga tagtggtctt tgttacccgt gacggtcagc cgttctctgt cgttcgcgtg
     3361 atggatgcat tcaaccctga actcatcacc catactctgg atctcatcga gtgtcttgac
     3421 gccggtggct actcgtttgc cagcatcatc agcacgctgt cacaggaggg cgcacaatga
     3481 tcccgtcatc cagtgctctt gtatccctga aaccagcccg tcaggcagcg ctgcaggcca
     3541 tcatgacggt ggaagaggct cgccagcgcg gcgcacgtct gccatcgatg ccacacgtcc
     3601 ggacattcct gcgcctgctg acaggctgta gccggattaa cagtgacgtt gcccgccgta
     3661 ttccgggcct tcaccgtgat ccaaaagacc gcctttccag cctgaaacag gtggaagagg
     3721 cgctggatat gcttatctcc agccacgggg aatactgtcc gctgccgctg acaatggatg
     3781 tgcaggctga actttttccg gaagtccttc atacccgaac agtacgcagg ctaaaacgtc
     3841 aggactttgc cttcacccga aaaatgcgcc gtgaagcccg tcaggtcgag cagtcctggt
     3901 tactgcgtca gaacctgctg ggacaggctg tcacagaact gaattttcag tcaccggaaa
     3961 ccgtctgcac ctggtacacc cggtggagcg acgagtttga cgcggcagag cttgcagcac
     4021 cgttctggcg ctggcagtcg cgattcacat cgctgaagga actggactgg cttcgcatca
     4081 gcggtgagcc gctgtatgca gtgatgtacg aaattccgtt aattgtccgg gaaacacctg
     4141 agcatattcg tgttgctgaa cgctggcagg tgccaaataa gctggctgat cggagtggtg
     4201 tatgaagtgg cgctattctc tgcgctggaa actgccgcac aggccctgcc cgggtccacg
     4261 ggaactgatt tccgttgtcg tggaggctgg gcaggcggca cctgaagagg tgatgtcacg
     4321 ctgggtggct ggctcaggtt acgctgtatg cgtggatttt cacggccaga aacagatcca
     4381 acgctggagc gatgaacgga aagctgcggt gagaaggcgc aacatgcagg ctcgtattca
     4441 tcgtgttgcc cctttgtttg ccgacgagtt aatcgagcgt gaactggctg cgcgtccgga
     4501 gtatttcaat ggcaagtccg cccgatgaat aagtacaatg ctgtctgaca actcagatgg
     4561 ccaaatacaa ggaaagcaga actatgaggt actggttgct cgttatctta atgaacgtgc
     4621 tgttctggtt gatcgtgaag catgttgctc cggaatggta tggttcccct tgttattatg
     4681 caggccagat tatctgcaca ctaatagctg cggtcatcac atggcgtgat ccgcctgact
     4741 ggttagatgg aatcctgctc ggcgttatag aattcggcgt tctgattgcc gggattatat
     4801 tctttgtctg gggcatcggg tacaagctgg cattctgggg gcaacatgat cctaatacca
     4861 tatatgcaag tgcaaacgat gtgattttgc ctaatcttgc gctgggcacg ggcgtcttga
     4921 ttgcgggatg ggtatacatt agatatttaa agcgctaagc agcataaagc gtggatatac
     4981 ctggtaagaa atagcaccgt cccggtataa tcgacgtggc gcttgaggct ttttgcctca
     5041 tgacgtgaag gtggtttgtt accgtgttgt gtggcaaaaa gcagaaagcc ccgtagttaa
     5101 ttttcattaa cccacgaggc cacctgtatg cctagacaac atcaggatag cctcttagat
     5161 gccgcaaggc aaggagaaga aggccatgaa actgccgcga aacactctgc tcgggtgcgt
     5221 attaatagtg tgcctcacac tgctgatatt cacttacctc acccggaaat cactttgtga
     5281 aatccggtac agggacacga acagggaggt ggcggctttt ctggcttacg aatccgctaa
     5341 gtagctaacc ccggcggggg gaagcccccg ccattcaggg gagatgatgt ctgacaagcc
     5401 tgaagcgcct ttatgaaggg gttaccgttc accggtagcc cctttttcct gcttacagga
     5461 aaaaaagccc gcacgacagg cgggcaaaaa tctttcatct acctacaaca cagattcaat
     5521 gaatcgcaag tcacattaac atccttcatt caccgtaaca ccctgtcatc agtctgaaaa
     5581 aaaagtgatt gattgtgaac atttgggttc tggtgataag ttctctctgt agcgtagccc
     5641 tctttacgga ggggtggaaa cagcaacggc agagttaaaa gaccacgagt cagtgaggct
     5701 gtccaaaggc tccccgggga aacccggcgc tacatcaggt tgcatagcac ttaacccgcc
     5761 cgcctgccgg gttttccttc cggaaaagtg ctgctataac ttattcgtgc aatgcgcggt
     5821 atccgctttc ctggtaaatc agtcgcccgg tctgccaggt gatactgcag ttcattcctt
     5881 atatgataca gtgatgttcg cctgcttttt aagcaggctt tttttgttga ttatgtatga
     5941 catctgtcat acagttgatt gcgtaatgaa cagaaattgt tccacttaac tcagtgaggc
     6001 gaagcgcagt cgcgggtgag ccaaaggaga gccagcatga acttcacgat aaaatcccgt
     6061 aaaaccggcg aaattttcag tttttacgca cccgacagtg gtggttatgt tcacctggag
     6121 tccccggggc accctggtaa caccggcgcc cagatttgcc gtggtggagg gtttatgggg
     6181 agtacgcttt cctgtggcgc ttctgaggac gatctggcca gtgttgcccg taaatggtac
     6241 cgacagttcg tgagggagcg gcgcaaattc ttaattatgt cgggccagta ttctgaggat
     6301 aaccagtgaa aaaagagatc tcccgcaacc cctctttcac accttcgcct aagctgcgag
     6361 cacatctgaa ctcccacagg gaaggggtga ctgaacggct gaataatatc tttgatcgct
     6421 atgcgcatct ggttcgcgcc tgtgcccttc ctctcgatga cgacgaaaca caggttctgc
     6481 ttaatgttct gaatggctcc gttgtggaac ccgcctttat tgaatatctg gctcaggaaa
     6541 ttcgggactc ggatgattat ctggaaggga tacccgcagc taaatcactg tacgaaaagt
     6601 gtcagtcagc gacctatccg caattgctgg ccaccgtcga acggctcgat cgctgaagaa
     6661 ggtaaatccg atgagtgacg tactttcctg cagacaactt acggctaatc ttaagatgat
     6721 tgccggggcg attggctgcc tcaacaggaa cgatgttgca caaattatca gcctgggcgg
     6781 tgttccatgt tcaaaaagcc gggccgactc gataatccgc agtgccgggg cggaaaaaaa
     6841 tgcatccggt aattctcatc ttcgcggagc cagaataaaa cgatccgcag atgtcacacc
     6901 ggaagaattc aatgcgtttt gtgcagggct gaaaacattt cttgtctcgt ttgaaacgaa
     6961 taatgtgagt gaaaacaatg ataagtaagc tcctgacctg tctgtgctcc ggacaaatgc
     7021 agtttcagga attaataaat ccctatatat ggcagcgtta attttgtgac gcggattatt
     7081 acatccagcc cgcttattga gctgtagata ttgatttatg ttgattgttc tcgattcgtc
     7141 cattggatca gcccgttatt tctctgcggg cttttgttct tatattcctc cgttccttcc
     7201 tgtgatggct ttatttcctg ctgtgtccct ttttaaaact gaaaaggatt aaaagcacac
     7261 ccacactgga caccttcggc tcctggccag ctgcgctgtc cagtcgtcga gccacactcc
     7321 gccagggcag ctgcttcgca cccgccgggc tccgccgaac ccgcaagcgg tttcgggcct
     7381 gacggctagt gtctgatgtc tgaggtcagg gtcaacggca gcgcttgctg cgaccgttcc
     7441 ggccgcgccg ctttcaccgt gccggcgata aagctgccgg cactctgcag ggcaacgccg
     7501 tttcgcgccc gcgctcttcg tcgttgcggc ggatgcctgc tcctttcccc tggcgccgta
     7561 tcacccgttt gagtccattc gccggggctg agacaagcac agtcgccatc atccgtccag
     7621 ggtaaatggc acgcggtaaa ccgcgccctg gacagctgct gtcgcccgtg ctcgtttacg
     7681 ccctcgcggc gaacggctct caaacgggtg aaggttttat ccagggggca ggttcagcag
     7741 gtccccgcac aaagtcaaaa ccgcggcccc ggcaactcaa cgggtgatgg ttttaaaagg
     7801 cagaaaaaca gacaaaggag aaagctggat gagcacacaa aacgtcaacg taaaaccctc
     7861 caccaaagaa agcacaaccc tccggactga cggttttgtg cgcaacattc attcccgaaa
     7921 cccgttcgat gttatccgcg ccgatgtagt gctggaacgt atcgagaaag aggctggttg
     7981 gtgctgcggg atgcattacg agctgtatca ggcccatctg ctgggtggtg cgctggatta
     8041 tctggatgcg ctgccgttga aagaccgtcc ggtgttaatg ggcgctgcgg caaagcgcgg
     8101 ctatctgctg acgctagccg aagaggaacg cgcgctggaa gcacgggatg tactgatgag
     8161 tgaactggcg gcaaacgact gctgagaaat aaacacggat ggcggcgtat cgctgccatc
     8221 ccttaaagac cagaggtaac gcaaaatgaa agtatacggc catcaacact catcagaggt
     8281 tttctcccgg gaaacttccc catcctgttt tgaaccaaaa gcggaaaagt tgatcgcttt
     8341 tcatggaaag gatgccagtt acgcctataa cgtgctggcc gatctgaaag ccgcaggtgc
     8401 ttcatatacg cgccggaata tcatcgccga tatgttccgc cgtctgggtg tggaatgagg
     8461 tctgggcaat ggaacacgtg aaaagacaaa tccagcaggg ggcagggaac gcctattatg
     8521 cggggatgca gtggcgcggt attacactcg gacacgccga tgctgcagcg cttaatcagt
     8581 ttgatatcga tatcagcaaa gctcaggcac ccgaagcacg cacctggttg cttcagaaca
     8641 aagcggaatt catagctctt atgctgggta ttgagattgt aaagatcggg tagtgagaag
     8701 ttagctaact tagctcttga gctaagttag cttttaagct aacgtataca catacggacc
     8761 agagattaca ttcatgttca cagtcatctt tcatgatgag gctgaaaaag agtttacggc
     8821 tctgcctgct gccattcgtg caaaaatggc caggctactc atgaagctgg aagcaaaccc
     8881 tcgccagcta cgcgagccag ataccaagcc gcttggaaac ggccttttcg aaatacgtac
     8941 aatgggggca gatatcgccc gcggaatctg ggtatatcag agtggtgaac gtatttttct
     9001 acttcggatc tttatcaaaa aatcgccaaa aacgcccccg gcagaaatag atctggcact
     9061 tcgcagactg gaggaaatgc agaatgaaat ataaaaccct tagccaggtt cataccgaag
     9121 caatgaacga tcatgagtac agcgcggcat tcgaggcaga agaagcctcg gagctacttc
     9181 gggaaacctt agcaacatgg agaaaagaag cgggtttgac gtcggcacag gttgcagaac
     9241 gtatgggcat caaagcgcct actatctcac gtatggagaa gaacgcttcg cgaatgagta
     9301 ttcagacgct ggttcggtac gccagagcct gcggtgttaa ctttaaaatt cagcaggttt
     9361 gaatgtcact cgtcagccgg atcctcagtg gtgaacgcag tctgaccctc gatcacatgc
     9421 gggcgctggc aaatcgattt caaatcccgg tctcgatgtt tgttgactga ttcgggaaca
     9481 gagcaggcga agggggcatc tgtccccgtc tggccgcgcc tcgaaaggcc gcgcgcctgc
     9541 ggcgccggga aaaccgggcc gcgctgacgc gccgcccgca tttcctttcc gcgtcttccc
     9601 tgtttgctgc ctgctgtctg tctgcttcct cttcttctcc ctgctgtgat gcctcttttt
     9661 aacccttaaa ggctttaaaa gcacacccat actggacacc ttcggctcct ggccagctac
     9721 gctgtccagt cgtcgagcca cactccgcta accgcgaaaa gacgcggtaa gctccgccga
     9781 acccgcaagc ggtttcgggc ctgacggcta gtgtctgatg tctgaagtca aggtcaacgg
     9841 cagcgcttgc tgcgaccgtc ccggtcgcgc cgctttcacc gtgccggcga aggactgccg
     9901 gcactctgca gggcaacgcc gtttcgcgcc cgcgctcttc gtcgttgcgg cgggtgcctg
     9961 ctcctttccc ctggtacccg tatcacccgt ttgagtccat tcgccggggc tgagacaagc
    10021 acaacccgtc ttgctgtcca ggggacggca gagcctttcg caataaattt ttcccggcca
    10081 ttccgctgcg cttcattaga ccgaaaaaaa tttcccgctc acccccggac atctgcaccg
    10141 ggccgtgctc gtttacgccc tcgcggcgaa cggctctcaa acgggtgaag gttatatcca
    10201 gggggcaggt tcagcaggtc cccgcacaaa gtcaaaaccg cggccccggc aactcaacgg
    10261 gtgatggtta aaaaattaaa caggtgaagg agaataatat gcgtttagca tcccgatttg
    10321 gacttactca ttccattcgt caggaacgcc cactgactaa cgatgaatta gtcaaggttg
    10381 taccttctgt gttttcagaa gaaaagcata attcacgtag tgatcgctat acctatattc
    10441 caaccattac actgctggat aaattacgtg aagaaggttt ccagccattt tttgcctgtc
    10501 agtcacgagt aagggatgag gataaacggg ggcatactaa gcatatggta agacttcgcc
    10561 gtgaaggggc caataaagga acagaagtac ccgaaatcat tcttcttaac tcgcatgatg
    10621 gatcgtccag ttatcaaatg atcccgggta tgttccgttt tgtatgcaca aatggccttg
    10681 tgtgcggtac gtcgtttggt gagattcgtg tacctcataa aggtgatatt gttggaaggg
    10741 ttatcgaagg tgcttacgaa gttctgggaa ttttcgataa aataactgaa ggcgttgatg
    10801 tcatgaagtc tattgcctta accaaggaag aacagcgtct gtttggacaa gctgcattaa
    10861 cctaccgata tgaagatgaa aataaatctc cggtcagtat tgaacagata attcatccgc
    10921 gccgttatga agataaaaaa gatgacatct ggactacata tcaaagggtc caggaaaatc
    10981 tgattaaagg cggtttgcct ggccgaactg aaaaagggaa aagaacaaca acccggccag
    11041 taaaagcaat cgatggtgat gttaaactca ataaggcatt gtggttaatt gcggagaaat
    11101 tccgcactct taaaggctaa ttataagcgc cgggcagctg tccggcgttt tgatggtggg
    11161 aatacttatg tttgaattta aagctgtctg tatcagtatt tcacatatgg aagaaggtga
    11221 tatcagagtt cttcaggtgc tttcatcaac aaccaattat ctaacttgcg atagttactt
    11281 agagtggata catgatacac cttatggttg tgttataagg atgtatgatt tcaaacgcca
    11341 tcttctggcg ctaaaaagag gtggggtgtc aaaattttta cgcaaaactc ttatactaat
    11401 gcaccgggag ggtgggtatc tgctttttca ttttgatagt gatgccgatt attatccagg
    11461 tatagatgcc gtagattttt aagaaattca agccttctta tatcagaagg ctttttagtt
    11521 catggcgatt ttctgatctt tagaagaagt tcgtttgctt gctttgatag ccgtgtagta
    11581 gtaatggatc ttcttcgcat actcgtaacg tcttgctgcc tggcgcgggg tctttttgaa
    11641 gcctgcattg tatgccccga ctgcatccca cgaaacgccc cactttttaa acgcctgagc
    11701 cagataatag gctccggtgt agatgttaag acaagcatcg ctaagaagat gatccggttt
    11761 tattccatat cgcgacagtt cgttgaaatg ctgcgagtca atttgcatca agcctgatcc
    11821 ataacccgta acaggattac gcccaatcgc atcaatttta aatcctgatt cttgccatga
    11881 tatagcacgc aggagatccg gatcaatttt gtaatccctg ccagccatat caaagcagtc
    11941 tgctgccatt gatgacaatg gtattaaaga caggatgata ccaaacgcta ctcccttcat
    12001 gaattcccct ccgtgccaaa tcccggaata accttaaccc atccaagacc gagactcaac
    12061 atatatgcac tcctcaccac aaaatcacca cacactacgc aaaattaaaa ttttgcagat
    12121 ttcactttag cttcagtggc ttaactgtag ttatattaca ttatgttatt tttctttata
    12181 tatatgtgag ttgccggtga gtcagtagta agtcgtcggt aagtctatag cgactcgttt
    12241 atgactcact cttttataat catatattta aagcgactta actctagact taagtcgcca
    12301 tatgactcgt aatgcaactt tcttagactc tagacttaag tttagagttg taaagaaggt
    12361 aaactcgcgt tatcttctcg ccataataac ttgtttgatg ctgtttttaa taaaggaacc
    12421 gatatggcca gggtacaagc ttatgctagt gatgaagttg cagagaaaat taatgcaata
    12481 gtggaaaagc gaagggctga aggggcaaag gaaaaggatg tcagtttttc aagtgtcgct
    12541 tcgatgctcc ttgaattagg gcttagggtt tatgaggctc aaatggaaag aaaagattca
    12601 gggtttaacc aaactgagtt taataagatt ttgcttgaga atgtcatgaa gactcagttt
    12661 accgtatcca aacttttggc tattggctct ctaagccctc atgttacagg tgatgaacga
    12721 ttcgagttcc gcagcatggt gagtaacatt cgagaagatg caaaagatgt tatttctcac
    12781 ttcttccccg agcaggaaga agagtaagaa ctggtgtgtg tttatggtgg tttgtaatta
    12841 attcagcagt aaaaaaagaa taatgatcta gctttaaaaa gtgacatgga agtataagga
    12901 atatagaata cttacagggt tgtactaaaa tttgttgaag cgttgacgtc cggggcttgt
    12961 ccccccgaac gtcaactatc catagtgtcc tttccaggag gcgcctatgt gtgcgaagga
    13021 tcgtagtcat attgctgtgc gcagtcaaac agattccatt cattctatgc gcgagatgct
    13081 taatatttca tcttgccctg cttttattcg tgattcccgg ggcgaagtaa ttcacaccag
    13141 cccaaccttt gacaaaattt tcttaacatc tggtggaact ggagcatttg atacacccag
    13201 agcaccggga agctggtttc tggatctctc cctggagatc aaactggaac tgatgcagtc
    13261 agaactgaaa tcgttttcag aaggttctgc tgtacttgtt aaaaatattt ggttggcagg
    13321 ttcactttgg actgttttta ttgaaacgtt ctcgattaat gaagatacat acagtaaatg
    13381 ggtttttata aatgaagatg aaccgatacc tcatccttca atggaataca atgacttttc
    13441 tataaagatg caaagatata tcgaaagaat tcagcgttca gcaaagtctg actgggctat
    13501 ctttaatctg tatgcggttg ggttttctca tgcatctatt tctaagatta caggtgttgg
    13561 ggtgcaaacc tcaaaaaata cagttagtaa aataaaaaag gaactgagct ttgatgatcg
    13621 tgactatatc ataatgtcat cgatctatac tctaagttat ggaaagttta tatcgaatgt
    13681 ggtttcgata ttaaaagacg gtgttaattt tttgttaaat caaaagtacc atcggtactt
    13741 ttgatttgtg aaaataattt actcaagatt tctccttagt ttgtttactt ttaaatcctc
    13801 caatgtaaga tttattaaag gttggtaatc ttactttgga ggtcgattgg aaagagcgat
    13861 taggagcaac gcacgacctg cgcctggaaa aaatgtgctt ttaaaattag atgaagaaac
    13921 taatcgtcga ctcatcaaag caaaagacag aagcagaaga agtaaaacag ctgaagctta
    13981 tttgagactg aaagatcatc tagaacgttt tccggacttt tataactcgg aactagccgt
    14041 accaggaggt gaaaaggaag agtgatttaa catcatagtc aaaaatctgt tttatatatc
    14101 aaccattctt tttaaaggaa ataaatatga atgctgtatt aagtgttcag ggctctgctg
    14161 tctctgaaaa aaccaaaccg tcttttatgt cccgcgtttt caataaaaag aaagccctga
    14221 aagtcgctaa agttgcgctg ccggttgctg ctgtggcggc gttcttcccg gatgctgcga
    14281 tggccaccac cacggcaacc gacctgatga agtccgggga tgcgaccgtt aaaggcacgt
    14341 ttggtaagca gtccagcgtc gtcaaatggg tggttctggc agaagttgtc gccggcggca
    14401 tcatgtacat gatgaccaaa aacgtgaagt tcctgtttgg cttcgcgatt atttccacct
    14461 ttatcaccat cggtatgtcg gtcgccggtt actgacagga agcacgtgat gacgggagac
    14521 gaattaaagc gataccgttt cccggaaaca ctgactaacc agtcccgctg gtttggctta
    14581 tatctcgatg aacttatccc ggccgtgatt tgtctcggct ggggtttctg gaccagcaag
    14641 ttcatttttg gcatagcttc agcggtactg gttttctggg gtatcaagaa actgaaaaaa
    14701 gggcggggca gttcctggct acgcgacctg atttactggt atctgccaac ctcccttctt
    14761 aaaggtttct ttcatgatgt tccggactcc tgtttccggc agtggattaa ataaatcaac
    14821 ggggaaataa catatggaac atggtgccag acaaaatacc acccgcgtta tcgctgtggg
    14881 atttatcagt ctggggacac tcctgaccct cagtcttgcg accaatatta tccagggcat
    14941 aaataattac cgtctgcaga cggaacagaa agtggcggtg acaccgatgt tattccgtgc
    15001 accctttgcg gtgtctcaga accaggctga cgcctcttat attgagcagc tgggcctgtc
    15061 ctttgttgcc ctgcgtctga atgtcacgcc ggaaacggtt gatgcccagc atcagcagct
    15121 actccggtat gtattacccg cctcgcagaa cagcctcaag gtccagctgg ctgaagatgc
    15181 gaaacgcatt aaagacaata acgttaattc caccttttat atgacgtcga tgcgtgcctg
    15241 gccggcagag aaccgcgtgg atatccgggg tgagctgaaa acgtggattg gggattccaa
    15301 accctacagt gaaattaaaa gctatgtcat tcagttcagt cgtgtcgatg gtgtcagctg
    15361 gctggcacgt tttggagaaa taaataatga aaaaaattaa cccgttattt atttcaggct
    15421 gtctgctgtt ggccgccccc gcgatgtccg cgaccctcag cggtacgctg gcgccgacag
    15481 tggtccccct aaccaatggt ggtcaggcaa atattgccgt gagtaacacc gacccgaatc
    15541 tgttcacagt acctggcgat cgcatcacgg cgataaacag cctggacggt ggcctgacaa
    15601 accaggagca gaccgacagc ggcggtgcca ttctggcgac cgtcagtaaa aaaccattca
    15661 ccttcattgt ggaaaccgaa cgcgggctga acttctccat tcgtgccgtg ccccgtgcgg
    15721 gctcaggccg gacaattcag ctggtcagcg agctggcggg tacccccggt ccggcaaaag
    15781 cctgggaaga gtcgaacccc tatgaatcgt tactggtttc gctgaatcgc gccgtgcgtc
    15841 agggcagcgt acccgatgag tatcagtctg tccccgtcac ttctgaagtg ctgcaggtgc
    15901 cggctggtct gcgcgccact gccgatcggg tctgggtcgg tcatcacctg aaagttgtcc
    15961 gttacagtct ggacaatgtc tccctgtcgg cccgtatggt gcgtgaaagc gatttctggc
    16021 agcccggaac acgtgccgtc atgttcagta ccccggcggg cctgctgact gccggtggtc
    16081 gcatgcagat ctgggtcacg acgtcagatg aaggagtgaa gcgctgatgg ccaacttcaa
    16141 cacgctgatc aaacgtaagc aatatatctg gctgggactc attctggcgg gcggcgcggc
    16201 agcagtcgga ggcgggctgt atctgtcaga tctgaacatg agctctgatg aggaagcccc
    16261 ggctcagggc gaacccgcac cggatatgac cggggtggtc gacagcagct tcaacagtaa
    16321 ggtggagcag cacgcgacaa cagaaatgca ggcgactgcc gctgatctca acaagcgttt
    16381 tgactccctg cagggggaag tggaactact gagtaaggcg cgaaccgctg accagatacg
    16441 tatcgacaaa ctcagcagtg acaatgaagc catgcagaat cagctgaagt cgctgggcgt
    16501 gaaaccggct gtctctggtg gcgagcccgc gccgactcct ccgtcaccgc cacccggtcc
    16561 ggaaggggag ccgcagccag ccagttatcc gccgcaaacc ggcgccgcgg taccgcctcc
    16621 gacggcattc tatccgggga atggtatgac accgccgccg caggtcagct accagtctgt
    16681 accggtacca aaccagatac agcgtaaaac gttcagttac gacaaaggaa aaaaaacgaa
    16741 gtcattgccg tacattccgt cggggagctt tgcaaaaagc atgcttatcg agggggcaga
    16801 tgccaacgcg tctgttaccg ggaatgaatc aactgtaccg atgcagttgc gtatcaccgg
    16861 tcgcgtggaa atgccaaaca gcaaaaccta cgacctgaca gggtgttttg tgggccttga
    16921 agcctggggc gatgtctcca gcgaacgcgc cattgtccgt acccgcaata tcagctgtat
    16981 caaaggtgat aaaaccatcg atcagcccat taacggccac gtttccttta tggggaaaaa
    17041 cggggttaag ggagaggtgg tgatgcgtaa cggaaaaatc ctcggctggg catggggggc
    17101 cggttttgtt gacgggattg gtcagggcat ggaacgggct tcacagccgg ctgttggcct
    17161 gggggcaacc gcatctgttg gcgccggtga cgttctgaaa atgggggtcg gcggtggtgc
    17221 atctaaagct gcgcagacgc tgagcgagta ctacatcaaa agggccgagc agtatcaccc
    17281 ggtcattccg attggtgccg gcaacgaagt gaccgtcgtg ttccaggatg gattccagct
    17341 gaaaacgatt gaagaactgg aagcggaaaa gaacgggcaa cagcaggctc agaatcagga
    17401 ggcggagccg gcacagcagg ctgatgcaca tcatcagtca ggcggcggta tgaacggatt
    17461 caatactgac gagatgctga agaagctggg caaacttgat ccgcgtgagt tctctccggg
    17521 cgcagcgccg cagggggaaa ataatggcta gttcgggcga ttaaggaaaa tacccagctc
    17581 aggatgaacc tgaaaaaaaa ccgataaata gcgagaaata ttatgaagga attaatgctg
    17641 ttaattcctc tgggcagcgc tttgctctta accgggtgcg cgggcacaga gactgaattt
    17701 caatgtaatg ccacgacgtc ggatacctgt atgacgatgg agcaggcaaa tgaaaaagcg
    17761 aagctgaagg aggaacgcag tgatgcaaag ccggctgcgg ccgggttgcc acaactggct
    17821 gagggtaatt tcggaaccac gtcagttaat ccgtacccgt tgccgcctca acccaacctg
    17881 aaccgggcca gagtggcagc ggagagagaa cgagcacgtg cagtgtacct aaataatcca
    17941 acggcaaaga atgaggcaat atatttcgaa accgggaaaa ggctccagtc tttgccggta
    18001 gtgaaaatgt ctttaccagt agtggcgtca acgtttacgt caccgtcaac accgccaggt
    18061 aattatcccc ggccattaag aaaaggggag gagacaacgt cattgtggat agcgccgtat
    18121 gtggattcag atgatgtata tcaccagccg gcaacggttc tttttgtcgt aaaaccatcg
    18181 gcgtggggac aacctcgcct taattgattc acagcgccat tactggcgct gcagtaagtt
    18241 ttcatttctg aattataacg agtgccctct gcgttgaaag ggatgggcgg tagcgtgttc
    18301 agacgaagat attacgctca tgtatcttta tgtaagggtt atttaaaagg gcgggttgat
    18361 gataaagggt ttttctctcc aggcaaaatc gacttcagtg tagtgatgtt attgaaaatg
    18421 taagcaagtc tgattctgac agagcgataa gtggcgatag gcaaacaact atcgcagagg
    18481 caatgaaaga aatattcatt cgttaatata taaacagagg caataaagta tgcatgcctt
    18541 tatttttctg gtgctgatga cgccgagtag tggcggtggg atttgggata ttactcccat
    18601 gcctaataca gatgtctgca taaaaatgct acgggtactc gaagcacgtg ggggcagagg
    18661 ttacggaagt aatacaggag agtgcattga aattaaaacc cgcgagcctt tgagcagcgt
    18721 gatagaaaca ttgaatgagg gtgggtaaat ggctgaatac cagttaggtt ttgatggttt
    18781 tgttcgactt ttttttgcaa tggcaatcat tatctgcggt tacggctatg taattattgg
    18841 tgtttttata agagatacgc tttcctgtat tttaatgttg tttgggctta caggtctcag
    18901 tgctttattt tcattaatgg tattcatgga taaaaacatg tgggccggct ctcctgattt
    18961 gacgctgtca gtaataacgg gattttttac agccacagtg gttttttata tgttttatgt
    19021 cttgatttat tactttgatc ggatgggggc aggtgaatag taagaccgat ggtcagtatt
    19081 aacaatctca cgttatttga aagtatgcag cataaggaat aagaaagtga aagcgtacta
    19141 tatacttgga cataacgttg catggcttaa cggtatatgc ctgattcttt ttgttattgg
    19201 tgtggtcggt gcgctggcaa tggttgccat ccctgaaaaa ttcaatcttc gagttaacag
    19261 aggcgataca ttcatatact gtgcgcttat agcggtggtt ggattttccg ggatgttcgt
    19321 tatctccatt cactcatttt cgatggatga acttgaagca ggtagacact ggaaagatga
    19381 ctgccggaca cttgaggtaa acatgccgac aggcgctttt accagccctg ttaataaact
    19441 ggactgtgat ggcatcatca ttaatgtgcc tggtgggcag tattattcct atatccatca
    19501 gtgggaatta tataaagcta actcaaaatg aaatgctgat taacaaactg gattgaccgg
    19561 tagggatgga acaatggaaa gacagcaaga aattggccag caatttggtt ttttgatagt
    19621 tcttctttta attgtgttaa ctgggtattt tttttgtgca gtatattgaa gattctagcg
    19681 atatgcagcc cgctactcca gaacaactgc aaagtattat tcattagaca ccttgtgcag
    19741 gtgaggcgtt acgtttagca attcaaacta acacaggcac ttcgtcagag ccattaactt
    19801 tcggtgacgc aaaaaagatg gcatcagaat gcaagtataa agaagagatt aaagcagtac
    19861 gtgaaaaaaa cttaacgcac tgaacgctat gagcaaataa tcaggggatg gatatgatgc
    19921 tgtttcttcg aaagtacgga tttttgttta tacctccagt ctactggctc atctatctgg
    19981 gagccagact gacttacaat ggactggcag gagagttttc tcctgatttc tttcagggta
    20041 ttcgacatga aggcgtctat attatctgta gtttgtgtgt attttatttt ttctgcttag
    20101 aggtggaagt tggtgatggg gagttctcag cgaacattct gttcctgatt gttttctttc
    20161 atgtggtttt attatttatc actctgctta tttgtctgtg gcaaggggag gtgttgttgg
    20221 attcgatctt tatgggtcag attattgctt ccatgtgttt attgtttttt attccagtgc
    20281 tttccattat aagaaggagt ttctagtgcg gatatgaata ttcttcttgc catttattac
    20341 ccttgttatt ttgaaatgct cttaaaggaa cgcctgcatg agtaataaca tcatcgatgc
    20401 cgtcacccag actgtgaatt cactggtatc agcactgaag ctgcccgacg aatccgcgaa
    20461 agccaatgac acgctgggca gcatgaattt cccccagttc agccgtattt tgccctataa
    20521 ggattatgat tccgcgaccg ggctgtttat taataacaaa accatcggtt ttatgtttga
    20581 ggcgcgtcct ttacccggtg cggataaaag tatcgtcgcc acactggagc atttactgcg
    20641 cagcaaactt ccccggggcg tcccggtcag ttttcatctg gtctccagca aactggtcgg
    20701 caatgatatc gactacggtc tgcgtgaatt ccgctggagc ggtaaacagg ctaagaagtt
    20761 taacgccatc acccaggcct actatctccg ggcagcggag acgaagttcc ccctgcctcc
    20821 tgcactggat ttacccctga cgctgcgtaa ctaccgcgtg tacatctcct gctgcgtgcc
    20881 ccggaagaaa aactccacca cgcaaattgt ggagatggag aaccagatta aaatcctgcg
    20941 ggcgtcactg ggcggtgcct atattccgac gcgtatcctt gatgctgccg ggctggtcga
    21001 actgatgcgc gagctgataa acccggaccc gcacgagatg taccgcgtac cgtataagct
    21061 ggacccctat caggatctga actatcagtg tgtcgatgac agctttgata tgcaggtcac
    21121 cgccggtcat ctgaaaattg gccgcctggg gcgcgacggg aaagagtgcg taacccgggt
    21181 gacgagctac catctggaga gtgatccgga aatggccttc ctctggacct ccgcggataa
    21241 ctacgccaac ctgcttaacc cggagctctc catctcctgc ccctttgtca tcacgctgac
    21301 gctgatggtg gaggaccagg tgaagaccca gaatgaagcc aacatgaaat tcatggacgt
    21361 ggagaagaaa agcaaaacct cctacgcgaa gttttttccg aacgtgataa aggaaatgca
    21421 ggagtggggc gatatccgcc agcgactggc gaccaaccag acctccctgg tgtcctactt
    21481 ctttaatatc accacgtaca ccgcagacag cacagaggcc tctctcgccg ctgaacagca
    21541 ggtgctcaac agctaccgta aaggcggttt ccagctgatc ccggcccgct accatcacct
    21601 gcgtaacttt ctggccatga tgccgttcaa gtgtggtgag ggcctgttta aagagctgca
    21661 ggcggcaggc gtggtgaagc gggcggagac gttccaggtg gctaacctcc tgccgatcgt
    21721 cgctgacagc ccgctggcgc cggcgggact cctggcgccc acctaccgaa atcagctggc
    21781 gttcatcgac ctgttctacg agggaatgaa caataccaat ttcaacatgg cagtctgcgg
    21841 gacgtccggg gcgggtaaaa ccggtctgat tcagcccctc attcgcagtg tgctcgactc
    21901 cggcgggttt gcctgggtgt tcgacatggg cgatggctat aagtccctgt gtgaaaacat
    21961 ggggggcgta tacctcgatg gcgataccct caagtttaac ccgttcgcca acgtgctgga
    22021 tgatgctcac tttgacatgt cagcagaacg tatacgcgat cagatgtcgg tgatggccag
    22081 tccgaacggc aacctcgatg aagtccatga aggtctgctt ctgcaggcag ttcaggcggc
    22141 ctggttaagc aagcgtaatc atgcacgcgt tgatgacgta gttcagtttt tacaggacgc
    22201 gaaggacagc gatgaatatg ctgattcccc gacaatccgg ggccgtctgg acgagatgat
    22261 aatcctgctc gatcagtaca cggtgaatgg gatctacggg gactatttca actcagataa
    22321 accaacactt cacgatgacg cccggatggt ggtactggag ctcggtggcc tggagtcgcg
    22381 gccctctctg cttatcgcgg tgatgttctc attgattatt tacatcgaga acaggatgta
    22441 tcagtcaccg cgtggcctga aaaaactgaa cgtcatcgat gagggctgga agctccttga
    22501 tttcaagaat gaaaaagtgg ggcagtttat cgagaaaggc taccgtaccg cgcgtcgaca
    22561 taccggcgca tacatcacca tcacgcaaaa tatcgtggac ttcgactccc cgaccgcatc
    22621 gagcgctgca cgcgctgcct gggggaactc gtcgtacaag gccattctga agcagtcagc
    22681 caaagagttt gccaagtata accagctgta cccggatcag ttcagcaagc tggagaaaga
    22741 catgattaac ggcttcggtt cggccaaaga gcagtggttc tcctctttta tgctgcaggt
    22801 ggaagcgaac tgctcctggc accgcctgtt cgttgacccg ctcagccggg cgatgtactc
    22861 ctccaaaggc ccggattttg agtacatgca agcgcgccgg caggaggggg tcgatatcca
    22921 cgatgcggta tacggactgg cgtgccgcaa ctttaaagac gaaatggcag agcttgaatc
    22981 ccgaataccg gtgaatgaca tggaggataa acagtgatga aaaacaacac cgaacaaccg
    23041 gtcagtacga cctctcttac ctttcgccgg atacctgtca tcgttgctct cgtgcttttc
    23101 gccctggcaa ccagcgcgtt tctctcacgc gtggttctgg actactccac gccgcgggtg
    23161 gtggcttttg atatgaagaa aacgctcgac agctttatgg acagtgtgag tcagaagcag
    23221 ctgacagagg cgcagtccaa agcgctctct gatcggttta acgacgcgct ggagaagagt
    23281 ctggcggagt atcagcagca gcatcatgtg gtcattctgg tttcacctgc cgtggttcag
    23341 ggcgcgcctg atgtgacccg caacatccag cacgacattg cccgccggat gaaaggagaa
    23401 gagtaatgat acgactgaaa gactgtctgc tcgccgcctg cctgttatcg ccgctgactc
    23461 aggcggctga tctcggtacc tggggggatt tatggcccgt ccgggagcag gacatgctgc
    23521 agctcatcac gcagcgtctg cagtcgctgc aatcctctgg ccagtggggc cagacgatgg
    23581 atgcgtttaa gcagcgggtt atcgagaaca gtcagcgtcc ggcgccggtg gaaggcatta
    23641 agcgtgcgga gaaatacgag cagcgctggt ttgatcccag catccgactg accgaagacc
    23701 tgaaggacaa tgaggggcgg gtgtttgccc gcaagggcga agtggtgaac ccgcttaaaa
    23761 ccgtaccgtt tgtccagacc ctgtacttca tcaatggtga cgatgccgac cagctggcct
    23821 ggatgaagcg tcaggtgccg gaaaccctga tgagcaaaat tatcctggtg cgtggcagta
    23881 tcccggacac ctccgcagcg ctggacagcc ggatctattt cgaccagaac ggtgtactga
    23941 gcaagcgatt tggcctgacg gcggtcccgg cgcggatcac gccggcgccc tccggtgaac
    24001 ggctgaatat tgagaccttc ccgcccgtcc cgcacccctg atctggggct gcgttttatt
    24061 caaaagaggt aaccgatgcc gatgatactg gccttaataa tcctgctcac gatgctcttg
    24121 ctgacgctaa tgtggtccac catccgtaag caggagaatg tcatacggga actcagtaca
    24181 agagaaagct ggctggagag agaggttgag cgtctcagga ccgataacga gaagctgtac
    24241 cgctggctga gatacaacgg tgaaaaggtt atcgatgaaa tgacttgtga actcgatgca
    24301 ctgtccgccg ttcgccagac gaacagggat tcataccgtc gccagcttta cctcttcgac
    24361 tgcctgtccc tgatggtgtg ggccgggatg gagggcgccg aagactctac acgggaaaag
    24421 gtccgcaata aaaccgatgc cctccgtctt gttctcaggg agaaaactga cgaatgaaag
    24481 ccatattttt tttaatctgc atcgcgttct caacgttcag tggccgcgcc gccgcagacc
    24541 cctcctgtga gggccggttt gttaacccga tcaccgatgt gtgctggcgc tgcatatttc
    24601 ctttatcact cggcagcgtg caggttggta aaggcgatct acctgatacc agcaatccgg
    24661 gtagtcccct gcagctgtgt ccggcgccgc caccgatttt tgttcgtccc ggcctggcca
    24721 tcgggtactg ggagccgatg gccatgacgg acgtcagccg ttcgcctggc tgtatggtca
    24781 atctgggggg cttcagtatc aatctcggca agaccggtat ggggaccgcg agaaaggacg
    24841 ataagcaggt gaacggcgct ttttatcacg ttcactggta caagtacccg ctgacctact
    24901 ggctgaacat catcacctcc gcaggctgtc tggaaggcgg agacatggat atagcctatc
    24961 tgtccgagat tgacccgacc tgggtggaca gcagcctcac caccattctg aatccggaag
    25021 ccatcctgtt tgccaacccc atcgcacagg gggcgtgcgc ggctgacgct atggccagcg
    25081 cgtttcatat gccactcgat attctgttct ggtgtgccgg ctctcagggt tcgatgtacc
    25141 cgtttagcgg ctgggtaagc aacgagtcca gtccgctgca gtcctcgtta cttgtcagcg
    25201 agcgcatggc gtacaagctt caccggcagg gacaaattat ggagagcatc gggaaggaca
    25261 aggcggtctg ctatgagtat ccgtccccga tcatccccaa agagcgctgg cgctatcaga
    25321 tggtgaacat gtacccggac agcggccagt gccatcccgt ggggcgcagc gtgatgcgct
    25381 gggaggccgg taaaaatcca cccaacaccc gtaagaacta tggctatctg atgtggcgta
    25441 aacgcaattg tgtctttctc tgacaggagt ctctcatgaa attaatgaat ctgtttatgg
    25501 tcatgctttt gctgaccgga cgagcagtgt tggctgaaac ctcctctgtt acagacagag
    25561 aatggctgaa gcagcaggag aacctcagcg aacagctgcg ccagcatccg gataaacagc
    25621 tgcagcagga gcttgaggcg caaatcaacc gtaatcctct gcccaaatca gaccgccagt
    25681 ttatcgataa tctggtcagc cagcagaaag cggcgaatca ggaaaagccg gcagaagggg
    25741 cgctgtactt tgtctccttc tcgatacctg aagaggggct gaagcggatg ctgcacgaaa
    25801 cccggcagta cggcattccg gcgacactgc gtgggctcgt caataacgac atgaagacca
    25861 ccactgacgc ggtgctgcag ctggtgaagg atggcgtgac cgacggcgtg cagatcgacc
    25921 cgacactata tacgcagtac agcatccgga gtgtgccgtc tctggtggta cggtgtcagg
    25981 ccggatatga cgtggtacgc ggtaacattc acgtcaaaca ggcgctggag aaagtggcac
    26041 aaaccggtga ctgcgcacag gtggcccggc aaatacttga tgcgccctcg aatgccgccg
    26101 ggagccagcc atgaaagata aactgtactt catggcgtta ccggaaatgg tgagggatga
    26161 gggagggtgt aaatgaagac ggttatttcc gtgctgaccg cgcatttttt tgtcctgagc
    26221 gccttcatct ggctggcatc ccccgcctgc gctgacagtg gaagtgacta taaagccggt
    26281 tcggattttg cgaaacaggt gcagggcaat ggtctgaact cactaaaaaa tttcagtggt
    26341 gagcaaaacc tgccggggta taccgacaac ccggatcaga caaaatacta cggcggcgtc
    26401 actgccagcg gagacagctc tctgaaaagc gattcagccc tggagttcag ccagggagat
    26461 accggcaaaa cgattacgga gtcgttcacg aaccggccac cggatcagat atctcaggat
    26521 gcgccgttta ttcaggccgc aaaggataca gaatcccgtg ccgacagcat tgtcggtgat
    26581 accggacagt cctgtaccgc ccaggttgtg aaccgcagcg agttcacaaa ccatacctgc
    26641 gaacgtgatc tgcaggtgga aaatttttgc acacgggaag cgaccctgaa ggataacgcc
    26701 accacacaga aagttaaccg aacctatcag caggtggtga cgctgaatta tgcccggagt
    26761 acccggcaat ggagtggcaa cctgacgatc ccgaccaacg gtcgtctgct gaatgcctcg
    26821 gtggatggtg agcctctggt gatcccctgg attgaagaat gcgattccga agggaaggtg
    26881 cgggatagct gcaagagtgc agtgagtgaa agcctgaccc tctttgaacg aacgttcccc
    26941 attgatgtga tcagctggcc acgttcagaa tcgatgtgtt caggcggtca gaacacgcat
    27001 tgtacgaaat atacctatga cgggaagggg aagattcatc agtcgtttgg tgtggacaaa
    27061 gcggtcattg ctgggcagaa cttttcagta agcaaaacat cacggaccgt atcatcggcc
    27121 agccagaaac ccgtacaggt cacggtcacc ctggtgatgg aagaaaccga aaccgtctat
    27181 gcacctgaag tcgtctgggt tgaaagctgt ccgttcagta aggatgaagg gaaaaaaacg
    27241 ggcgaggaat gtatcagtcc gggaggaacg cgaaccatca ccctcggagg cagggattac
    27301 tcgttcacgg aggcctgctg gaaatataag gatacctggc tcacgcagcc ggcggacaac
    27361 ggctcctgtg aaagcctgat gaaaaatacg gcctgtactc tgtcatcccg ccagtgcgcc
    27421 ttctccagcg aggaaggcac gtgcctgcat gaatatgcaa cgtattcctg cgaaaccagg
    27481 acgagtggca agcaaatgat ctgcggcggt gatgtcttct gccttgatgg tgaatgtgac
    27541 aaagccacgt ccgggaagag caacgacttt gggcaggcgg tgtctgagct ggcggcactg
    27601 gctgccgcgg ggaaggacgt cgcggcactg aacggggtgg atgtccgggc attcaccgga
    27661 aaagcgaagt tctgtaagaa gtttgctgcc ggcttcagta actgctgcaa agacagtggc
    27721 tggggccagg atgtggggct ggcaagctgc agcagcgagg aaaaggcgct ggccaaagcg
    27781 aaaaaagaca agctgacggt cagtataggg gaattctgct ctaaaaaggt tctggggatt
    27841 tgcctggaga aaaaaaggag ctattgccag ttcgactcaa aactggcgca gatcgttcag
    27901 cagcagggaa gaaacggtca gctgcatatc ggttttggcg gcgccagcag tcctgactgt
    27961 cggggcatta ccgtcgcgga actccagggg attgatttta acaagctgga tttcaccaac
    28021 tttatggacg acctgatgaa aaatcaaaaa ataccggaaa acgatgtgct caccaataag
    28081 accagagaac gaattaagga aatcatgagt cagcaatccg cgcaataaac catgctgcct
    28141 aataaagaga ccgataatca tgaaaatgag ttttcacaaa aagcctgtta ttgattttgt
    28201 cctgagatta acgctaacaa taatcattat ttctccggtg atttatttct cctgggatgc
    28261 agtcaaaggg acgtcgggaa gtgattatgt cgaaagtgtg gtctttattc ttatggctgg
    28321 gtttgccatg tctgtatgtt atttgttttt ctcagtcctg gagaaagtta taaatgcaag
    28381 gaaaagcaaa gatgaatcaa tatatcacga ttgagaaatt tatagacatt ctgaatgaag
    28441 agaatttacc ccaggagcat catgttatgg tgctggctgt tcttgcggat atctctctgc
    28501 atacagacag gttcctgatt aactcgtccg aactggttca gatggccgct cagtactcac
    28561 ctgctttcca gaaactgcca gcggaccggc aggcttttat atcttcagta ctgagcatgc
    28621 cgctattcct tattatgtga ccaggagagt caatggaaga gaaaaaagca tacgggctgg
    28681 tcatggtgtt tgtcggggtg tttgtctttc tcctggtcag cattatgtcg tacagcctgt
    28741 ggcgggacag gcaggttaac gcatttatga caaccaaccg ggcctggggg atccagtgcg
    28801 acactgtttc tcaggccgcg tgggtgatca gggacgggga gcgggtggac cttcagataa
    28861 attatctgcc tctgtactgc agcggttatc gctttgaagc ccgtgatgat gcaggaaagg
    28921 tacagcgcca gctcgataaa tacagtgtct atcagcatct gtcacgccag tcgcattaat
    28981 tcataaaagg gtatttgcca tgcgaacaat acatgccgcc atgctggcgg ggtgcctttg
    29041 ctgcgccctg atgccggctt tcgccaaaga cgcgggctgg cagtggtaca acgagaaact
    29101 gaagccccgt gatgaggaca ggaaagccgt accagcggca ccaccgccgc aaatggatat
    29161 tctggagaaa ctcgccacac tccaggcggc caccaaacgg tcgttgtacg aagccattct
    29221 gtatccatcg tctgaaaact tcgtgaagta tttccggctg cagaactact ggacgcagca
    29281 ggcaggtttg ttctcgatga gtgcgaagaa agccatgctg gaaaaccccg aactcgatta
    29341 caacctgcag tacagccact acaacggcac ggtaaaaaac cagctcgctg ccgactatgc
    29401 cgaacagcga caggctatct ccaccctcgc acagcactac ggcgtgatgt tcttctaccg
    29461 gggccgggaa gcgatcgatg gccagctggt ccaggtgatt aaaaattttc gcgaaaccta
    29521 tggcctgagc gtgataccgg tgtcggttga cggagtgatt aaccccatgc ttcccgacag
    29581 ccggacggac cagggccagg cagagcagct tggcgtgaag ttcttccccg cgatgatgct
    29641 ggttaacccg aaaagtggcc aggtgaagcc tctcagttac ggctttatca gtcaggacga
    29701 cctggcgaaa cagttcctct atgtctccag cgactttaaa ccgaatttct aaggagtgtg
    29761 tcatgcgaaa tattcgtttt ccggatttag atatcaccgg gatgtgggtg ctggccgtgg
    29821 gcgtgttttt ccacctgata gcccggcttg tcagaaaaca gcctgagctg gccgtccagg
    29881 ccggggaaat cttcgggctg ggtatggtgg tgttcggggg ataccgcatt ctaaacgccc
    29941 tgattgctga agccgaaaaa gaggagaaag cctgtgaaaa acagcctgat gaacctcgct
    30001 aagacgccgc tggcggcact ggctctttcc cttgcactgg ccggggtggc acatgcggga
    30061 acgctcgatg aggtgaagtc gctctgggat ccgcggggta tctccggtac tgaaacggcc
    30121 tccccgcaga gcgacactcc ccaggcggtg agcggggcat ctcagccacg ctggttacga
    30181 ctcagcaacg gaaagcaggt ggacctgcgc gactggaaag tggtgctgtt catgcaggga
    30241 cactgtccct actgtcataa gtttgacccg gtgctgaagc agctggccgg acagtatggc
    30301 ttctccgtgt tctcttacac cattgacgga cagggtgatg acgcgtttcc ggaagcgctg
    30361 ccggcgcctc ctgacgtgat gcagaccttt ttccccaata tcccggtggc cacgccgact
    30421 acgtttctgg tgaacgtaaa cacgctggca gcgtacccga tcctgcaggg cgccacggat
    30481 gcacagggct ttatggccag agtggatacg gtatttcaga tgatgcactg agtctctgtt
    30541 atatccggtc ttagcaagtg attaaggaaa atagccatga acaaatatat acgttcgacg
    30601 ggactgtatg cgtttttatt tccagccagt ttaaaggact ccgggcagac agcagcagaa
    30661 agaatagaac aactgaagcc ggaatttatc caccgggaaa ggcgtctgga aatctatctg
    30721 gaattattca ttgtgtttct cactgcaggt gctctgctgt tatggataat gagattttta
    30781 tttaatctct gcgtggatga ctggatagcc agcggtgacc tgcgagtaaa agacctgtgg
    30841 aatattatga tgtacgccat cccttatgcg ctgatcgctg ttggggtggg gttctttgtt
    30901 gccggagtga cattagcaat ccgcaatttt ttcagttatc accttaagac cctttttatt
    30961 ctgagaaatg acagagttaa aaagaatgct gtgcgtaatg gaggacagga tgcgaattaa
    31021 acttctgtca cttttctgcg gtgccgcgct tggcctgtca tttagcgccg ccgccgacgt
    31081 gaatggcgac atgaataact tcttcaataa gctcgggttt gcgtccaata ccagccagcc
    31141 acaggtctgg caggggcagg cggcgggtta tgcctctggc ggttcgctgt atgccagaac
    31201 gcaggtgaaa acgatccagc tggtcagcat gaccctgccg gatattaacg ccggctgcgg
    31261 cggcatcgat gcgtacctgg ggtcgttctc ctttatcaac agcgagcagc tgcagcgctt
    31321 tggcaaacaa attatgtcca acgccgcggg ttacttcttt gaccttgccc tgcagaccac
    31381 ggttccggag atcaaaaccg caaaggactt ccttcaaaag atggccactg acatgaacag
    31441 catgaacctc agttcctgcc aggctgctca gggaattgtg ggtggcctgt ttcctcgtac
    31501 ccaggtcgct caacaaaagg tctgccagga cattgccggt gaaagcaata tcttttcgga
    31561 ctgggccgcc tcgcgccagg gttgtacggt gggcggcaag atggacagcg tccaggataa
    31621 ggccagcgat aaagacaaag aacgtgtgat gaagaacatt aacatcatgt ggaatgcctt
    31681 atcgaagaac cggctctttg atggcaataa ggagctgaaa gagtttgtga tgaccctgac
    31741 cggtacgctg atttttggtg agaacagtga gatcaccccg ttgccggcac gcaccactga
    31801 ccaggatctg attaaagcca tgatggaggg cggcacagct aaaatctacc actgcaatga
    31861 ctcggataaa tgtctcaagg tcgttgctga tgcgaccgta accatcaccg ccaataaggc
    31921 gctcaaaaat cagattcgcg ctttgttgag cagtatccag aataaagcgg tggctgatga
    31981 aaaactgaca gagcaggaga aagggtttat ctccagcacc accattccgg tgttcaaata
    32041 cctggtcgat ccgcagatgc tcggggtgtc taactcactt atttaccagc tgacggacta
    32101 tatcggctac gacatcctgc tgcagtacat tcaggaactt atccagcagg cccgggcgat
    32161 gatctcaacc ggtaattatc cccagtcgac aatggatatg atcatggaaa atctcaacca
    32221 ggcgagtgtg cagattgccg ctttccagtc ccgtgttcag gtccagcagg acgccttgct
    32281 tgtggttgac agacaaatga gttacatgcg ccagcaggtc tctgcccgca tgatgacccg
    32341 ctaccagaat aactatcatt ttggggggaa tctgtaatgc ttgagatata tgccattgct
    32401 ggcggcgact ggttacgggg caacctgaac gcgattgcgg cgtttatggg gaccagcaca
    32461 tggtcgacta tagaaaagat gtgtatcgcc atctcggtac tcattgtggc ggggaactgg
    32521 gtaaaaaaac acaacgtaat ggaccttatt ggctgggtct tttccctcac cctggtctct
    32581 atgctggttg tcattcgtac ccctgtgcag atcatcgatt acagtaacgt tgcgcaggtc
    32641 tatgaagtcg ataatgttcc tattggtctt gccattcctg cgtcgctgac aacgagggtg
    32701 ggaaacgcgc ttatccagag ttatgagatg gtcttcgcgc tgccggacag cgtcacgtac
    32761 agtaaaacgg ggatgttatt tggcagtaat ctggtcgcga aaagtacgga ttttttgtca
    32821 caaaatcctc agattacgac gctgttttcc gattacgtac aaaactgtgt gatgggcgat
    32881 attttcctca atcataagta ctcttttgaa gaattactga attcacctga tccgtatacc
    32941 ctgatttttg caaacccaag cccactgcgt ggcgtgtttg ataaaaacaa ccagttccag
    33001 acatgcgaag aggcctcacg ggatctgaaa tcggctcttg cgcttgatac tcagacaggc
    33061 gggaaaacct ggaactacta tgttcgtcag ctgtttggcg gcaaacctaa tcccgatctg
    33121 cttttcagcc agatgattgg cgacagctac aattacttct acagctcggg acagagcgcc
    33181 gggcagatta tccgccagaa cgtcacgatg aacgccctgc gcagtgggat ccagagttat
    33241 gcggcccgca gcggggacac cgccagtctg gtcaatatgg ctaacacatc atcccttgaa
    33301 aagcagcggc tggcacaggc cacaatgggg caccaggcgc ttcgcgcact tccactgatg
    33361 cagaccgtca ttatgggttt aatgattggg atgttcccga tcatggtgat ggctgccatg
    33421 tttaacatga tgacgctgca ggtgcttaaa gggtatgtgt ttgcgcttat ctggctgcag
    33481 acgtggccat tactgttcgc catcctgaac agcgcgatgg cctactatgc gaagcaaaat
    33541 ggcgtgccgg ttgttctctc tgagttgtcg caggttcagc tcaaaaactc ggatattgcg
    33601 accaccgcgg gttatattgc cgtgatgata ccgcccttgt cgtgggggat cgtgaagagc
    33661 atgggggccg ctttctccag tgcctacagc catttttctt cttcggggct cagtgcaaca
    33721 tcgcaggcgg cctccggcgt ggtggacggg aattactctt tcgccaacat gcagatggag
    33781 aacgtcagcg gctacagctg gggtaccaac agtacaacca gtttcggaca gatgtcgcgg
    33841 cagctggcga atgggggtat gagcacgcag acgcgggatg gatcgatggt ctgggacagt
    33901 ggtggtgcaa tgtcaaaact gccggtggat atcaacgtcg gtcgtcagat agccagtgcg
    33961 cagcaacaga tggcccgtga ggctgatgtc caggccgaaa gcgcgctgca tggctataac
    34021 agcagcgtga caagtgcctg gaatagtctg cagcaatttg gcaccaataa agggaacagc
    34081 acatcaacaa cgacaggggc cgatactacc gagtcttcgc aggattcgat ggccagaagc
    34141 aagatgtgga atgcggttgt tgctaatgcg aaagccaaca acatcagtaa tgaggagtct
    34201 ttccagcagt tgatggatga ttcatcgaaa agaactcaat catttgatgc acatgtatct
    34261 ggtaaatgga gttctgggga tcagctgttt ggcaagttag gaaagtgggg cagtggtttt
    34321 tctgctgaag ttggtggtaa aggaagtgca ggctggggac acagctcagg taatacagat
    34381 aacgttggaa cttccggtag ggaatcgaat gacagccgac atgataccag cagtcaggcg
    34441 gccaaagact ttaaggaagc atccgattac tttaccagcc gcaaaaccac cacatcgggc
    34501 aatattaccg ataataatgc cagctcgcgt gttgaccagt ttgccgcgtc actctccagt
    34561 gccaaaaaca gctatgacca gtatacaagc agccggacac gcagccatga gtacagtgag
    34621 atggcatcac gcacagagag catgactggc cagatgaacg aaaacctgac tcagcagttt
    34681 gcgaactttg ttcagcatcg tgctccacag gatgccgaag ccattctgac caataccagt
    34741 tctcctgaaa ttgcggcgca gcgtgaagcc ctggcgcgtg aattcgtcaa agagcaggta
    34801 gaacccaggg ttgatgctgc gtatcaacag gggcgtgaaa gcatcggaca gaatatggct
    34861 ggggtgtcag gtggtggtga caatggttcg gtaatggccg actacggaca aaattctcgt
    34921 cgtatcgatg ttatgacaca agatgcgggt attaaggata acgtgggtca gaaggtggat
    34981 aggatgatta cagagaataa acaacagcat caggaaaccc gcgaaaacat acaacggcaa
    35041 ggcgcagagg tgaaaagtga aaatgctgaa atggaaaaga accataaaac taaagcaaat
    35101 gagtttaaag gtgattataa tgaaagaaag agtaaggtaa attctttacc tggagctgac
    35161 tctcaggctg agttagagga aaaagcggcg aaaacgcaaa aggattttct tgatgggaaa
    35221 agatgaaaat gatgatgacc aataaaatcc tcattgaaga agtagatgag ctgaaaaatg
    35281 tgttttcacg aggtgaaatg gagataccat ctttttggtc atgtctatgg cctggagtaa
    35341 ttgttgccgc ctggaatatg atttgcgccc taattagtgt agggggcggg tatttgtctg
    35401 ttaagttcat attttgggtg ttggtgttcc ccggtgttat aagtcttatt gttctgcttg
    35461 gtgtggcaag tgcgcgttca ctttttttat ctgttcccag aacctataga atgcgctcgc
    35521 aggtatatcg gtttatatct agaaaagtta tggtctatgc acttgtgtat atggctttaa
    35581 ttctagcgct tgcactattt aatagagtgt attatgcctc tccatttcct ttttcgttca
    35641 tgattcttat tgcgacgatt tttgtatggt ttgttatgaa tattgatttt ggacgatatc
    35701 aactttcggt actctcatct gcgataaact ctttcagagc agaaaagttg ggggggggat
    35761 gatccagagg caacctatca attatggtgg tttgttaaca gcctaaaagt ttcctttgtt
    35821 attattggtt tcttagactg taattagttt tcacatctcg tttaaaattc gtatatagtg
    35881 aatgcgcatt ccgaaatcat aagagaatgt gtattcactc gtattgatta tgtcacaagg
    35941 attcaggatg aagcttaata aattcatggc tgtgatggtc gtcagctctg ctctggtgtt
    36001 gtctggatgt agtgcaatgg gaaccgctat taaaaagaga aatctggaag taaaaaccca
    36061 gatgagcgaa acaatctggc tggaaccttc aaataataag acggtttatc tgcaaatcaa
    36121 aaatacctcc gataaggata tgagtggcct gcaggcaaaa attgccagcg ctgtcacttc
    36181 aaagggatat caggtcgtca gtaaccccga tacggcgggc tactggatcc aggcgaatgt
    36241 gctcaaggcc gataaaatgg accttcgtga gtctcagggc tggttaagcc ggggttatga
    36301 gggcgctgtg actggcgccg ctcttggggc ggggattacc gcttacaact ccagttccgc
    36361 gggagcaacc ctgggagtag ggcttgcagc cggtctggtc ggaatggcgg ctgacgcgat
    36421 ggtggaagat gtgaactaca ccatgatcac cgatgttcag atcgcggaaa gaaccaaaac
    36481 acaggttcag actgataacg tggccgtatt acgtcaggga acatccggag caaaagtgca
    36541 gacaagtact gaaacgggta atcagcataa ataccagact cgcgtcgtct ctaacgcgaa
    36601 taaagttaac ctcaagttcc ctgaagccca gcctgttctt gaagaccagc tggcaaaatc
    36661 gatcgctaat atcctgtaaa cgttctattc cacggcccgg gtactccggg ccagactggc
    36721 atcacctttc cggcgtgtca gaattcattc gtcaaataat cagttaattc cacatctaaa
    36781 cggtgaacag ttgcgcaatt tatttgtgca tctgtgaggc gttgccgcct tcggcggagt
    36841 cttttcgtct ttatttgaac agaggttatt tatgacaata tctgtgcctg tcaggtctct
    36901 tgatacacat gaactgatgc ctggcgatat ttacccacat cctgcagcgg ggggaattga
    36961 ttccctgtca catcgcgagg tctgttctct cttcagtgac cacatcatgt atatctgcag
    37021 cgcgttagtt attcttctgc ttgttttcat cgcaggtgtc tttttccgaa aaataatgac
    37081 gtcttccgtg gagcgtaagc gaaacacggc gtcagttatt tccgatgcaa agccagaagt
    37141 acagagcaaa ccggaatact tccatctttc catatgtgtg gaaggcccgg ccagcacgga
    37201 tgactccgcc ttgtcctctc ttgcctcccg tgttgtccgc gaagcagaaa atctgtatat
    37261 ccccggcgtc aatcgccgcg acagcgctga cgacgtgttt gcctggtccg tgcagactca
    37321 gtttccttct gacaggctct tcggcacacg gattttcccg tcaaccggct ggtttgaacg
    37381 tccggatatg attaaacatc cggtgaaatg ctactgcgtc tgcctgcagt acacgactga
    37441 aacattgcca tccacactgt atgccctggt cgatgaggcc ggacggcagg tggcgaagtc
    37501 gtatattaaa ggcgggcact gccctggctc agagggctat accttctgca taacctctta
    37561 atagaagtca gtatccggcc tggccatcaa tggatccggg catgactgta atcctgtttt
    37621 gtgccggtgt atttccgctg attaatttgt ccttccgacc ctaatacttt actgacggat
    37681 tagtgccatg agtttcaatg ccaaagatat gacgcagggc ggtcagatcg ccaatatgcg
    37741 ctttcgcatg tttggtcaga ttgccaatat tattttctac gtgctgttta ttttattctg
    37801 ggtgctgtgc gggctgatgc tgatgtatcg gctcagctgg caaacctttg tgaacggctg
    37861 cgtatactgg tggtgtacga cgctgggtcc gatgcgcgat attattcgtt cccagccggt
    37921 atacaccatt cagtattacg gccagtcgct ggaatatacc tcagaacaaa tcctggccga
    37981 taaatatact atctggtgcg gagagcagct gtggaccagt ttcgtctttg cagccgtggt
    38041 ttctcttgtt atctgtatcg tgacgttctt catcgccagt tgggtgctgg gccgccaggg
    38101 gaaacagcag agtgaggatg agaataccgg cgggcgccag ctttccgata agcccaaaga
    38161 agtggcccgc cagatgaagc gtgatggtat ggcctccgat attaaaattg gcgatctgcc
    38221 tattcttaaa aactccgaaa tccagaactt ctgcctccac ggtaccgtgg gttccggtaa
    38281 atcagaagta atccgccggc tactgaatta tgtt
//
LOCUS       DACADA010000027        38189 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.027, whole genome shotgun sequence.
ACCESSION   DACADA010000027 DACADA010000000
VERSION     DACADA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.027"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            4..1659
                     /locus_tag="ISB17_001385"
     CDS             4..1659
                     /locus_tag="ISB17_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HAP1979336.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1750..2985)
                     /locus_tag="ISB17_001386"
     CDS             complement(1750..2985)
                     /locus_tag="ISB17_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAP1979337.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPELNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3132..3208)
                     /locus_tag="ISB17_001387"
     tRNA            complement(3132..3208)
                     /locus_tag="ISB17_001387"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3172..3174),aa:Pro,seq:tgg)
     gene            complement(3251..3337)
                     /locus_tag="ISB17_001388"
     tRNA            complement(3251..3337)
                     /locus_tag="ISB17_001388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3301..3303),aa:Leu,seq:cag)
     gene            complement(3358..3433)
                     /locus_tag="ISB17_001389"
     tRNA            complement(3358..3433)
                     /locus_tag="ISB17_001389"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:His,seq:gtg)
     gene            complement(3492..3568)
                     /locus_tag="ISB17_001390"
     tRNA            complement(3492..3568)
                     /locus_tag="ISB17_001390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3532..3534),aa:Arg,seq:ccg)
     gene            complement(3671..5056)
                     /locus_tag="ISB17_001391"
     CDS             complement(3671..5056)
                     /locus_tag="ISB17_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1979338.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5247..5987)
                     /gene="rffM"
                     /locus_tag="ISB17_001392"
     CDS             complement(5247..5987)
                     /gene="rffM"
                     /locus_tag="ISB17_001392"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAP1979339.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(5990..7342)
                     /gene="wzyE"
                     /locus_tag="ISB17_001393"
     CDS             complement(5990..7342)
                     /gene="wzyE"
                     /locus_tag="ISB17_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAP1979340.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7339..8418)
                     /gene="rffT"
                     /locus_tag="ISB17_001394"
                     /gene_synonym="wecF"
     CDS             complement(7339..8418)
                     /gene="rffT"
                     /locus_tag="ISB17_001394"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAP1979341.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8415..9665)
                     /gene="wzxE"
                     /locus_tag="ISB17_001395"
     CDS             complement(8415..9665)
                     /gene="wzxE"
                     /locus_tag="ISB17_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAP1979342.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9667..10797)
                     /gene="rffA"
                     /locus_tag="ISB17_001396"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9667..10797)
                     /gene="rffA"
                     /locus_tag="ISB17_001396"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAP1979343.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10802..11476)
                     /gene="rffC"
                     /locus_tag="ISB17_001397"
                     /gene_synonym="wecD"
     CDS             complement(10802..11476)
                     /gene="rffC"
                     /locus_tag="ISB17_001397"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAP1979344.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11454..12335)
                     /gene="rfbA"
                     /locus_tag="ISB17_001398"
     CDS             complement(11454..12335)
                     /gene="rfbA"
                     /locus_tag="ISB17_001398"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP1979345.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12354..13421)
                     /gene="rffG"
                     /locus_tag="ISB17_001399"
     CDS             complement(12354..13421)
                     /gene="rffG"
                     /locus_tag="ISB17_001399"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP1979346.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13421..14683)
                     /gene="wecC"
                     /locus_tag="ISB17_001400"
     CDS             complement(13421..14683)
                     /gene="wecC"
                     /locus_tag="ISB17_001400"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAP1979347.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14680..15810)
                     /gene="wecB"
                     /locus_tag="ISB17_001401"
     CDS             complement(14680..15810)
                     /gene="wecB"
                     /locus_tag="ISB17_001401"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP1979348.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15866..16912)
                     /gene="wzzE"
                     /locus_tag="ISB17_001402"
     CDS             complement(15866..16912)
                     /gene="wzzE"
                     /locus_tag="ISB17_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAP1979349.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16924..18027)
                     /gene="wecA"
                     /locus_tag="ISB17_001403"
     CDS             complement(16924..18027)
                     /gene="wecA"
                     /locus_tag="ISB17_001403"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAP1979350.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18267..19526)
                     /gene="rho"
                     /locus_tag="ISB17_001404"
     CDS             complement(18267..19526)
                     /gene="rho"
                     /locus_tag="ISB17_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP1979351.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19611..19712)
                     /gene="rhoL"
                     /locus_tag="ISB17_001405"
     CDS             complement(19611..19712)
                     /gene="rhoL"
                     /locus_tag="ISB17_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAP1979352.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19853..20182)
                     /gene="trxA"
                     /locus_tag="ISB17_001406"
     CDS             complement(19853..20182)
                     /gene="trxA"
                     /locus_tag="ISB17_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAP1979353.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20313..21578
                     /gene="rhlB"
                     /locus_tag="ISB17_001407"
     CDS             20313..21578
                     /gene="rhlB"
                     /locus_tag="ISB17_001407"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAP1979354.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21714..23198
                     /gene="gppA"
                     /locus_tag="ISB17_001408"
     CDS             21714..23198
                     /gene="gppA"
                     /locus_tag="ISB17_001408"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAP1979355.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPPQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFEQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFANRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23245..25266)
                     /gene="rep"
                     /locus_tag="ISB17_001409"
     CDS             complement(23245..25266)
                     /gene="rep"
                     /locus_tag="ISB17_001409"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAP1979356.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25353..25634
                     /gene="ppiC"
                     /locus_tag="ISB17_001410"
     CDS             25353..25634
                     /gene="ppiC"
                     /locus_tag="ISB17_001410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAP1979357.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25680..27155)
                     /gene="ilvC"
                     /locus_tag="ISB17_001411"
     CDS             complement(25680..27155)
                     /gene="ilvC"
                     /locus_tag="ISB17_001411"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAP1979358.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27305..28195
                     /gene="ilvY"
                     /locus_tag="ISB17_001412"
     CDS             27305..28195
                     /gene="ilvY"
                     /locus_tag="ISB17_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAP1979359.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28192..29736)
                     /gene="ilvA"
                     /locus_tag="ISB17_001413"
     CDS             complement(28192..29736)
                     /gene="ilvA"
                     /locus_tag="ISB17_001413"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAP1979360.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29739..31589)
                     /gene="ilvD"
                     /locus_tag="ISB17_001414"
     CDS             complement(29739..31589)
                     /gene="ilvD"
                     /locus_tag="ISB17_001414"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAP1979361.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31654..32583)
                     /gene="ilvE"
                     /locus_tag="ISB17_001415"
     CDS             complement(31654..32583)
                     /gene="ilvE"
                     /locus_tag="ISB17_001415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAP1979362.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32603..32866)
                     /gene="ilvM"
                     /locus_tag="ISB17_001416"
     CDS             complement(32603..32866)
                     /gene="ilvM"
                     /locus_tag="ISB17_001416"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAP1979363.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32863..34509)
                     /locus_tag="ISB17_001417"
     CDS             complement(32863..34509)
                     /locus_tag="ISB17_001417"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAP1979364.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34512..34562)
                     /locus_tag="ISB17_001418"
     CDS             complement(34512..34562)
                     /locus_tag="ISB17_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979365.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34649..34747)
                     /gene="ilvL"
                     /locus_tag="ISB17_001419"
     CDS             complement(34649..34747)
                     /gene="ilvL"
                     /locus_tag="ISB17_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAP1979366.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35100..36620
                     /locus_tag="ISB17_001420"
     CDS             35100..36620
                     /locus_tag="ISB17_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAP1979367.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36645..36983)
                     /gene="maoP"
                     /locus_tag="ISB17_001421"
     CDS             complement(36645..36983)
                     /gene="maoP"
                     /locus_tag="ISB17_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAP1979368.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37102..37941
                     /gene="hdfR"
                     /locus_tag="ISB17_001422"
     CDS             37102..37941
                     /gene="hdfR"
                     /locus_tag="ISB17_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAP1979369.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38037..38112)
                     /locus_tag="ISB17_001423"
     tRNA            complement(38037..38112)
                     /locus_tag="ISB17_001423"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38077..38079),aa:Trp,seq:cca)
BASE COUNT         9327 a        10446 c         9653 g         8763 t
ORIGIN      
        1 tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc agctcttcct
       61 ctcatagcca gcgcagcaga taccccgtca accgccaccg cacgcaaagg ctttgccgga
      121 tacgatcacc caaaccagta tctggttaaa ccggcgacca ctattgccga caacatgatg
      181 ccggtgatgc agcatcctgc acaagacaaa gaaacccagc agaaactggc ggaacttgag
      241 aaaaaaaccg gtaagaaacc gaacgtggtt gttttcttgc tggatgatgt gggctggatg
      301 gacgttggct ttaacggcgg cggcgtggcg gtgggtaacc ctacaccaga tatcgacgcc
      361 gttgccagcc aggggctgat tttaacttcg gcgtattctc aaccgagttc ttccccaacc
      421 cgcgccacga ttctcaccgg acaatactcc atccaccacg gcattctgat gccgccaatg
      481 tacgggcaac cgggcggtct gcaagggtta accacgctgc cacagttgct gcacgatcag
      541 ggctacgtca cccaggctat cgggaaatgg catatggggg aaaacaaaga gtcgcagccg
      601 cagaacgttg gctttgatga tttccgcggc tttaactcgg tatctgatat gtataccgaa
      661 tggcgtgatg ttcatgtcaa cccggaagtc gccctgagcc cggatcgttc tgaatacatc
      721 caaaaactgc cgtttagcaa agatgacgtt catgccgtgc gcggcggtga acaggaagcc
      781 attgccgaca ttacgccgaa atatatggaa gatctggatc aacgctggat ggaatatggc
      841 gttaagttcc tcgacaagat ggcgaagagt gataagcctt tcttcctcta ttacggcact
      901 cgcggctgtc acttcgataa ctacccgaac gccaaatatg cgggtagctc tccggcgcgc
      961 acctcttacg gcgactgcat ggtggagatg aacgatatct tcgccaatct gtataaagca
     1021 ctggagaaaa acggtcagct tgataacacg ctgattgtgt tcacctctga taacggtccg
     1081 gaagccgaag ttccgccgca cggtcgcacg ccgttccgcg gtgcgaaagg ttctacatgg
     1141 gaaggcggcg ttcgtgtgcc aaccttcgtt tactggaaag gcatgatcca accgcgtaaa
     1201 tctgacggca ttgtcgatct ggcggatctc ttccctacgg cgctggatct ggcggggcat
     1261 cctggggcga aagtagcgaa tttagtaccg aaaaccacct ttatcgatgg tgtggaccag
     1321 acatctttct tcctgggaac aaatggtcag tctaaccgta aggccgagca ttacttcctc
     1381 aacggtaaac tttctgctgt gcgtatggat gagttcaagt atcacgtcct gattcagcaa
     1441 ccttacgctt atacccagag cggatatcag ggtggattca ccggcacagt aatgcaaacg
     1501 gcgggatcgt cggtgtttaa cctgtacacc gatccgcagg aaagcgactc tatcggcgtg
     1561 cgccatatcc caatgggtgt accgctacag accgaaatgc atgcgtatat ggagatcctg
     1621 aaaaaatatc caccacgcgc gcagattaaa tctgattaag ccggcgctat cgctgagatc
     1681 tacctttgcc ggatgcgatg ctgacgcatc cgacaatcaa ccgcaggcgg ccacaaaatg
     1741 gctgcttttt tacttattca ccaccagcaa atgcgcctgc ataatgtcgc tggccggacg
     1801 accgtgcgcc agcaaatcaa ccattgcttt aagatatggc ggtagatggc ggaaataacg
     1861 ctgatacccg gcacacaaat aattcagctc gggtttgccg ctggcatcga gcataaagcg
     1921 gtgtttcggg cagcctcccc agcacgcttt taacacgttg caactgcgac actgtgccgg
     1981 taactgcttg aatttatctt caccaaatgc ctgctgttgc ggggaatcaa tcatttctgc
     2041 aatcatctgc tgaagcatat tccctagtcg atactgcgga taaacatagt gatcgcaggc
     2101 ataaacgtca ccgttgtgct caacaatcac cgagcgccca caggttggct gatgatggca
     2161 aaccgcgccc ggtgcgccga caaaattggc gaacgcccat tcgatattca ttacgaaaat
     2221 cttgccgacg tcgcgcttaa tccagtggtc gaaaatcgcc accagaaact caccgaactc
     2281 ttgcgggcac accgaccatt ccgtcagttc gccctgaata tcgccgggag catgtagctt
     2341 aagtccagca tgagcagccg tttcattagc caggcgctcg accaccggaa taaactggat
     2401 gaactcaaca cctgcatcac acagaaaatc atagacttgc aaaggttgta acgcactggt
     2461 gcgattgacg cagaccagca cgttatagtc gacatgatgt ttttgcagga gcgtcagggc
     2521 acgcatcacc agcttatgcg tgggtctgcc acctttggtc acgcgatatt gattgtggat
     2581 ctcagccgga ccatccagcg ataaccctac aagaaaatga ttttctgcca gaaatccaca
     2641 ccattcgtca tcgagcagca cgccgttagt ctggaagcta ttacttatct tcctgccagc
     2701 accatatttc gcctgtagcg ccacggcacg gcggtaaaac tccagcccga gtaacgttgg
     2761 ttcgccgccc tgccaggtaa aagccacttc gttttgggtt tcgctggcag cgatatagtg
     2821 acggacatac gcttccagcg tgtcatcgtc catatgcgtg actggctttt cgtggtaaag
     2881 ggattgtttt tcgagataaa aacagtagtc acagttcaga ttacaatcag aaccgctcgg
     2941 tttcgccatc acatgaaaag cacgcgttgg aacctgttgc tgcatggtcg ctccttgcct
     3001 gaagtcatcc ttcaataagt taagggcgaa atcataccgt ggcgcaatgc gttattacgg
     3061 gcgtttattg cgaagggaaa gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg
     3121 gggttcaaaa ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag
     3181 ttgcgctacc aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc
     3241 tttaaagtcg tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac
     3301 ctgaagctag cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg
     3361 ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact
     3421 gagctatagc caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg
     3481 ccgggactaa atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca
     3541 gcgctctatc cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac
     3601 ttcccacccc tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc
     3661 gttttgctgc ttattcctcc agtttatgcg ctttaccgtg gcgattaagc ccaaaaactt
     3721 tataaatcgc cgtcaccgtc agcataaaga tgatgccgac aaacagcgac atacgcgtat
     3781 cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg
     3841 cccaagggaa caggattgag cggaacggat ggctggcaat tgccgcttta tgcgcacgac
     3901 gaaaacgcag ctggctaatc aatatcacaa accatggcac catccccgga agcacgctgg
     3961 cactgtagac atagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc
     4021 caattagcag aatagcaata gataccgcca cacccgcaac cggtacgccg tgacgggaaa
     4081 ctttcgccat cgccgccggt aactgacggt ttttcgccag tgcatagagc atacgtccgc
     4141 aactgtacat gccactgtta cagccagaaa gcgctgccgt cagcaccaca aagttaataa
     4201 tgcccgccgc agcggtaatg ccgattttgg cgaaagtcag tacgaacggg ctgccgttgc
     4261 tgcctatttc attccacggg aagatggtaa cgataacgaa aatcgcgcct acgtagaaaa
     4321 tcaggattcg ccacagcacc ttgcctacag cgctacgcag cgtcacctgc ggattcttcg
     4381 cttcaccggc agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca
     4441 atgcggtcag gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc
     4501 taaaaccaat cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga
     4561 caatcatcac gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt
     4621 acaacctcac cgcagccaga ttcgccaacg ccaccagcgc caccgcgatc aatgcgggta
     4681 tccactgcgc catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg
     4741 agatccccac cgccatccac ataaaccagt aagaccaggc ggtgagatag ccaaagaacg
     4801 ggctcatata acgatgcgcg taaacggcga acgaaccggt aaccggttcg aggaacaaca
     4861 tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca
     4921 atacggatgg cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa
     4981 tggtgccccc cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg
     5041 gtttgttatc tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta
     5101 tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa
     5161 agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg gagtgaccac
     5221 ttcgtcgctt tacaaagaga ggaaaatcag aggttgccgg tatagtgcca gcgtaaataa
     5281 cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac
     5341 tccagcccca gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg
     5401 taggtcccgc caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg
     5461 aagatctcct gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga
     5521 atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat aaccatcctg gctgccaacg
     5581 atattcacat tccactggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta
     5641 ccgcccacaa gaaataccgg tgtcccttct ttgcctgcgc gcgccatcag ctcttcccag
     5701 agatcggccc cagcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt
     5761 acaacgctta tgccatccgc atatttaaat tcggcagcgt taattagctc ccggacctcg
     5821 gcgttatctt caatggtcag cattttttcg gcattaatgg caaccagcgt tccttgctta
     5881 agctgcccgt cagcgaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac
     5941 tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt tattcattat tatccttcaa
     6001 cctgcgtccg gagcgatgat tttgttcgtt tatgaatgag tccggcgctt tcaaaaagcc
     6061 agtacaacag ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagaccacgc
     6121 gtgagacaaa cgaatccagc ccttcacgcg ccagcacgat catattgaag atcgccccga
     6181 agcagaaact gtgcaatatc gcagccttat agcgattagt ctcgcggttg cccagctcat
     6241 acagccagtc gaaccatttg atgatcagtc caacaacaat cgccccgagc gggatgaaca
     6301 acgcgccgcc catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt
     6361 ggttattcag cacttcccag gtaaagtagt tggcggagtt cagcaccata ctcgggcgac
     6421 ccggccacag ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct
     6481 ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggtgaaaagg
     6541 tgtcgcgagt gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat
     6601 agcgttttaa cgccagccag aacatgccca caatccccag cacgcccgcc gccgccagca
     6661 tccacaacga aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga
     6721 tgatattggc gcgagtgccg ccgacaatca tataagtcag caagccaaaa gcgaccgtgc
     6781 tgacgaggaa aaacagccac gctttgctgt cctggcgcag aaagtagacc accagcatcg
     6841 ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggagaaga
     6901 tctgactgta ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga
     6961 cgcttaccag cgcgatgccc atcaggatca cccacgtgag attggtttcc acgcggttca
     7021 tggtaaacag cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg
     7081 tgacgtagta aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg
     7141 gcggcgcggc accaacatca aagcgaaaaa ccagcacgct ggtcagcggg aagccgaaga
     7201 aaaaggtgag caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa
     7261 actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag cagccagaca acaaacaggc
     7321 cactgaattg cagcagactc atgcgacctc cccggcggca atcgccaacg cccgctgcca
     7381 gccttgcaga tagttcgggc taaagaaggc aatggtgttt ttatccaccg acaccaactg
     7441 gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcaccggcaa
     7501 atgctgttcc gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc
     7561 cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc
     7621 aagatcgcac tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcagagt
     7681 ttgtagattt tcttcgctga ataactccag ccccgcctga cgaacttcct cgatgtacgc
     7741 ttcgttatta ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg
     7801 ataaacggcg cgcaaggcag caatatgttc attgctgcgg tcaccggagt tccccaccag
     7861 aatggtcatt ttcccttcac gttgccgatc attcgccatc atattgagtg aagggtccat
     7921 ccgcgtcggg aagtacagca gttctccccg cacctttgga tgcgttttgg caaaaaagct
     7981 caaatcaccg cgggtggcaa atacacagcc gactctcttt tgcgccaggc gacgtagcgg
     8041 gtaaaaaagc ttatatctca agccactgga aagctcgtac aggtctgccc cccagatatg
     8101 ccagtaaaac tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt
     8161 gaactgaccg tggaagaaaa aacgctgctg acggttagct tttgctttcg cgatgaccgc
     8221 ttccgccagc gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact
     8281 taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag
     8341 cgcgtcattg aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac
     8401 gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa
     8461 aaatagacga tataggttgc catataggcc tgcgccgcgc ccagtgcacc atgcgcaggg
     8521 attagccagt gggcaaatac catcaataaa gtgaactggc tgacttccgc cagaatataa
     8581 aaccgcagtg acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac
     8641 acatcaccca ccaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac
     8701 agcagccaga tagcaaaatc acgcagcaac cagacggtaa aactcgccgc cgccactgcc
     8761 ggcaagacga atttaagcga tttaaccact tcccgggtga tatcgcgctt ttccgttagc
     8821 cgcgacaacg tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca
     8881 tcggaaatac tgctcacccc ttgccagatc cccacctcat cccagctata ctgcgccgcc
     8941 agcagtttac gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg
     9001 agcgtaaatt tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt
     9061 aacgggatga caccacgttt gatcaacatg atggcggcag gaattaccac cagcgcggga
     9121 atcagcgcca gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac
     9181 gcgagaacgc caatcaagct gccaacaatc agagataacg cattacctgc ggcatcgcga
     9241 aagcctttca tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc
     9301 actaaacgca ccagcccctg atagtcggta ttaccaaaca atccctggct gattggcgca
     9361 gctgccagca caaaaaccag cgccatcagc gtagagaaac caagtaccat cgctgatgaa
     9421 gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt
     9481 acaccgttaa agatgccagc cccggcaagc acgccgagca cggtaatcaa ctggcggaaa
     9541 tttgccgcca gcccaagccc cgccggacca aatgacaccg ccagcaactt accgactagt
     9601 aacccggcac caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac
     9661 gacatatcag gagaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga
     9721 caggttgtag aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc
     9781 ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg
     9841 aaacaccgcc atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat
     9901 atcccgcagt ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg
     9961 cagctcgata cgcccatttt ttgccagagg cgccagtgca tcgtagtagt tttgccacag
    10021 cgccagacgc tgctgattga tacgatccgc tgcttccagt tgcgcccaca ggtacgctgc
    10081 ttgcagatcg gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg
    10141 accacggaag aactggctgc ggtttgtgcc tttttcacgg atgatctcgg ctcgttcgat
    10201 caacgcttta tcgttaatca gcgtcgcgcc gccttcacca cccgccgtgt agtttttggt
    10261 ttcatggaag ctaaagcagc caatatgacc aatggttccc agcgcacgcc ctttgtaagt
    10321 ggacatcacg ccctgagcgg catcttccac cacaaataga ttatgcttct tcgccaacgc
    10381 cataatagtg tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt
    10441 tttgtcggtg atcgccgctt caatcagcgt ttcgttgatg ttcatggtgt ccgggcgaac
    10501 atccacaaag acgattttcg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt
    10561 gtagctcggc atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc
    10621 cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag
    10681 ccactgctgg cagcgacggg taaaaccacc atcgccgcac agtttgccgc tacccattgc
    10741 cgactgcata tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg
    10801 atcacctgta taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt
    10861 taagcgcggc ggtgttgccc atctgggtcg ccacccgcaa agttgtttta ccgcgagcat
    10921 acgcccagtt tagcgccgtt tgcattagct cagcacctgc accgcgtcca gccagcaggc
    10981 caattcgcgc atctgtcgca ttgagttccc gtaaagagac ataaccgcga atatcgccgg
    11041 acgccgcacg taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc
    11101 actgtgcata aaagcgaccg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt
    11161 gcgcaaatgc ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta
    11221 ctgcaccgct atcactgaca ttgttcacgg gtagtgccaa atcaacttca ccttctatca
    11281 gggagaatcc cagctgttgc agagcatcca gttcacccgt atttgatgcc gcaattttgg
    11341 cctgcacccg tgaccacggc gctaaggcgt ctggcgtcag gagtgccgcg tcagggctaa
    11401 tacgcacaat ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac
    11461 tggcgcggac gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat
    11521 gaactggcag cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcaatctct
    11581 tccaggcagg caatcttaaa gccctggcgt ttttctactg tctgcacaaa agtgctggcc
    11641 tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa atccgcgccc gagcagttca
    11701 acggtcaggt tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca
    11761 cgctccgacg gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc
    11821 ccggtcaccg cccagtttga tttcggctgt tttggttttt cttccagcga gatagcgcgg
    11881 aaattgtcgt caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat
    11941 accgtcgccc cttccgtgcg cgccgcaaca tgacgcagtt tcggactgaa gccctgtccg
    12001 aagaagatgt tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg
    12061 atgataaagg cctgcgccag gccgtccgga ctgggctgtt cggcatattc cagctggata
    12121 ccgaactcac tgccgtcacc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg
    12181 atgatgagaa tttcgcggat accggccagc atcagcaccg acagcggata gtaaatcatt
    12241 ggcttatcgt aaatcggcaa cagttgctta gatacgccgc gcgtaatcgg atgcaatcgg
    12301 gtgccggaac cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct
    12361 ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca
    12421 ttagccagat accactgcac cgttttacgc attccacttt caaaggtttc ctgcggcagc
    12481 cagccaagtt cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga
    12541 cggtcagcga caaaggtgat caggtcacga tagtgcgcca cgccgtgcgg cttattcgga
    12601 gccagttctt ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg
    12661 tgaccaccaa tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg
    12721 cgggcgtgat cttcaacata cagccagtca cggatttgct gcccgttgcc atataccggc
    12781 agcggtttac ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg
    12841 taaggaccgt agttattcga gcagttggtg ataagcgtcg gcagaccata ggtccgcagc
    12901 caggcgcgca ccagatggtc gctgctggct tttgacgcgg aataagggct gctcggcgca
    12961 tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg
    13021 gtggagatat gatgaaaacg gaacgctgat tttttatctt ccgtcagcgc agtccagtaa
    13081 gcccgcgccg cttcaagcag tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc
    13141 gggccgtcaa tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc
    13201 tgatactcag tgaatacgcg cgccagttct gcccgatcgc agatatcaac tttctcaaag
    13261 gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc
    13321 gtatcgacca ccaccaccgc gtcgctcgtt tcgttgatga tataacgcac cagcgccgag
    13381 ccaataaacc cggcaccacc tgttatcaga atctttctca tcagcgccag actcctttgg
    13441 catcgacgac atactgctga tggacgttgt cgccattgat aactttgaac tgactatgat
    13501 cgaccagcat caccagcaca tctgccgttg ccagcgcctc gtcaagctgc gccagagtac
    13561 aaagcccggt cagtttcttc ggcaactggt ggatgttagg ctcaacaacc agcgtttcgc
    13621 cgctgtgcca ctgggcgatc agttcagcga tttccattgc cggactttcg cgcaggtcat
    13681 caatattcgg tttaaacgcc agaccaaagc aggcgatttt cagttcattg gcgcgtttat
    13741 cggtagcagc cagacaatca gccaccgccg ctttcacctg atcgataacc cagaacggtt
    13801 tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca
    13861 cgataaacca cggatcaaca gcgatgcagt gaccgcccac gccagggcct ggctgaagaa
    13921 tattgacgcg agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat
    13981 cggcacaaat cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt
    14041 cggtgagctt acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga
    14101 aaattttgta cagttcgctg gcgcgggccg aacaaaccgg cgtcatacca ccaatcacgc
    14161 gatcgttttt aatcagctcg accataacct ggcccggtaa tacgcgttcc gggcagtaag
    14221 caatgttgac gtccgccaac tcgcccacct gctgtgggaa agtgagatct ggacgcatct
    14281 ctgctaacca ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca
    14341 ctaacgcgcc ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca
    14401 tatctggctc atgatcgcct ttaaacggtg tgggtacagc aatcaaccag gcatccgctt
    14461 caactggcgt cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg
    14521 ccaaatcagg ctcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt
    14581 tgatatcgac accaatcacc tgtttttgcc ttgaggcaaa cgcagctgct gtaggcagcc
    14641 cgatgtaacc cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt
    14701 ttttaacgct tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg
    14761 gctcatagct tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg
    14821 ctgcttatcc gtgccgacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt
    14881 ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc
    14941 tgagtcggtc aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg
    15001 gggatcgatc agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac
    15061 gttcgggttg agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc
    15121 tgccagcgca tggcagattt cttcaaagcc acgaccgaag ctctcacgcc tgtgaccggt
    15181 caccagaatc atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg
    15241 cagcgtatcg ctgctcatca cctggtcacg cacccataac agtgcatcaa tgactgtatt
    15301 accagtaatg aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt
    15361 ttcggttgga gaaaaatgat acatcgccag atgcccggtc aatgtacggt tagcctcttc
    15421 cggccacggc gaatagagat cgcccgtgcg cagaccagcc tcaacgtgac caacaggaat
    15481 acgctgataa aacgccgcca ggctggttgc cagcgtcgtc gtagtatcgc cgtgaaccag
    15541 cacgacatct ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt
    15601 tatctctgta aggccttgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga
    15661 aaagagtttc agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc
    15721 ctcaaaaaaa ggatcttttg ccaacgcatg caccaacggt gccatcttga tggcttccgg
    15781 gcgcgtacca aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc
    15841 ctttgcgctc accgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag
    15901 ccccgatcag tcccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat
    15961 cgcgttttac cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca
    16021 gggttggacc aacattcagg gtgtttaaca tggcccgatt ttgatcatag tcgagatcaa
    16081 aggccggacc gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca
    16141 ggaacatttc tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat
    16201 tatgctgctc agcaattttc agcgcctgtt caatactgtt catccggcgg tcatagatgg
    16261 ctttcgccac ctcttcctga cgcttcacct gagctttcat ctggatagtg cgtgccgccc
    16321 atgcgccttt cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat
    16381 actgacgtaa caggttatta gcgtcaggcg cggtttcggc aataagcttc acgctgtcat
    16441 tgaccgcgcg ggtaaagtca ccgggaataa acacgatgtt gttaatcatt tcatccagca
    16501 acgccgcatc ggctttgctg ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc
    16561 agaactctct gcgggtatcc cacgaggcca gttgcataac aaactcttta taagcttcgt
    16621 ccatgaccga tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa
    16681 attgctgctg tgagtagtac ccgcccagca tattcaccgt tggacgatcg gtaatcgccg
    16741 tcgcgctcca ctcctgacga gcaaaaaaag tatacgccag cgcgattaac gcaaacgcca
    16801 gccccatgcc aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat
    16861 ccagttcatt ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct
    16921 catttatttg gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg
    16981 cttaataaag cgagcaactt tccaggcacg cttgatgcaa tatccataga ggaagaatgc
    17041 tagcaaaaag agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac
    17101 gccaatggaa gcgagcaatg ctgcggcaag ggtaatcagc acaaacgcct ggcgggaagt
    17161 aaacccggca cgcatgatca aatggtgaat atgctgacgg tctggagaga atgggctcat
    17221 gcctttacgc aggcgacggt acataatcgc caccatatcc attagcggaa tggcgataat
    17281 ccacaaagcg gtaaccgggc tgatgggatg agttttgccc tgggtcgttt cgagcaggat
    17341 ccagataacg gtaaaaccaa tcagcgtact gcccgcatcc cccataaaga ctttgtagcg
    17401 gcgacccagg ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa
    17461 gcaccagatt gcgagactgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa
    17521 cgagacgcag gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt
    17581 aatggccgcc cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc
    17641 ccaggagcca aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac
    17701 aatgccaaca gcggcctgta tggtggctcg gatttttacg ctgatatcaa aacggtcatc
    17761 cagcgcgcca atgaaaacaa gcacaccagc acaagcgaga tagagagatg catgcggaat
    17821 atagtagtcg acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac
    17881 gagaggtatc aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt
    17941 ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag
    18001 atcagtactc actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa
    18061 gtataaccac gaagaccttt attcagaaag gaaagtccgt tccgaaccta ccagcactat
    18121 gcgaaaatta accacttgat atgcaaattg cataacacac cagcaaattc caggaatttt
    18181 cctaacatcg ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca
    18241 cgtggcgttt ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca
    18301 ttggtcttgg tcattgccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc
    18361 gggtgaatga ttttgcgcag gatccacatt ttctgcagtt cttcctgagt cgtgagcagc
    18421 tcttctttac gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg
    18481 atcttacgag agaggtgcag ttccatgttg cctgtacctt taaactcttc gtagataact
    18541 tcgtccattt tagaaccggt atcgataagc gccgtcgcga taatggtcag gctgccgccc
    18601 tcttccacgt tacgcgccgc accgaagaag cgtttcggac gatgcagggc gttggcgtcc
    18661 acaccaccgg tcaacacttt acctgacgcc ggaacaacgg tgttgtaagc gcgcgccaga
    18721 cgagtgatgg agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttcgcc
    18781 ttctcgatca ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa
    18841 gcaacaactt caccttttac cagtcgctgc atctcggtta cttcttccgg acgttcgtcg
    18901 atcagcagaa ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc
    18961 tgcagcagca tggttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga
    19021 ccgataggtg atgccagatc cagtacgcga gcggttaaat cttccgtaga accgttacca
    19081 cgttccatac gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg
    19141 ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag
    19201 cgttcacctt ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg
    19261 aaacggcgga tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg
    19321 tctgcggaac ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag
    19381 atatcttcgc cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc
    19441 atacgagcca ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc
    19501 gtattcttta attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc
    19561 gaacttaatg ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc
    19621 atgtcttttc gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg
    19681 agtgacgagc cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat
    19741 tacaagattc aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac
    19801 tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca
    19861 ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcgcccact ttggttgccg
    19921 ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg
    19981 cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt
    20041 cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga
    20101 aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca
    20161 ggtgaataat tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc
    20221 gttgttggtg taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt
    20281 aaagcaatag taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga
    20341 agttttccga cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc
    20401 ataactgtac gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag
    20461 ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt
    20521 atcttctctc tcatcccgcg attgctgatc gcaaggtgaa tcagccgcgt gccttaatta
    20581 tggcaccgac gcgtgaactt gccgtgcaga tccacgccga cgcggaaccg ctggcggaag
    20641 ctaccggcct gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag
    20701 tgctggaaag cggcgttgac attctgattg gcaccacggg tcgtttaatt gactacgcca
    20761 agcagaacca cattaacctc ggtgccattc aggtggtggt gctggacgaa gccgatcgca
    20821 tgtacgatct gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa
    20881 accagcgcct caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt
    20941 tcgagcagat gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc
    21001 gtattaaaga agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc
    21061 tgatcgaaga agagtggcca gacagagcga ttattttcgc caacaccaaa caccgttgtg
    21121 aagagatctg gggccacctg gcggcagatg gtcatcgtgt cggtttattg acaggcgatg
    21181 tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc
    21241 tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc tgctgtgaca cacgtcttta
    21301 actacgattt acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg
    21361 ctggcgcaag cggtcattcc atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg
    21421 ctattgagac ctatattggt cactcaattc cggtaagtaa atacaatccg gacgcattga
    21481 tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg cacaggcaat ggtccgcgtc
    21541 gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaag aaaaaatgtc atactccaaa
    21601 tcattcaagc tgtatcaaga tggcaagaga atgaatcccg atgagtttac tgtcgtaagt
    21661 gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt
    21721 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    21781 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg
    21841 ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat
    21901 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc cccgcaaatt cgcgttgtcg
    21961 ctacggcgac attacgcctt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa
    22021 tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcgcgtctg atttatcagg
    22081 gcgttgctca caccactggc ggtgccgatc agcgcctggt ggtggatatc ggcggagcca
    22141 gtactgaact ggtaaccggc acgggtgcac aaaccacctc tttgttcagc ctgtcgatgg
    22201 gctgcgttac ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg
    22261 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc
    22321 acggctggaa agtgtgcgtt ggcgcttccg gcaccgtgca ggcgttacag gaaatcatga
    22381 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaactg aaacaacgcg
    22441 ccattcattg cggtcggctg gaagagctag agattgacgg gctgacgctg gaacgtgcgt
    22501 tagtgttccc gagtggtctg gcgatcctga tagccatttt taccgaactg aatattcagt
    22561 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggtatg ttgcatctta
    22621 ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg
    22681 atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttcttcgaa caggtggaaa
    22741 atgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    22801 aaatcggcct gagcgttgac ttcaaacaag cgccgcaaca cgctgcttat ctggtgcgta
    22861 acctggatct tcccggtttt acccccgcac agaaaaaact gctggcgacg ctactgctca
    22921 accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg
    22981 tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccaac cgtcgccgtg
    23041 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    23101 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgatcag gaaagccagt
    23161 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    23221 ctgacacatt ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    23281 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc
    23341 ctgttcccaa atcaaatcat cctgcggcag ctccagcagg aagcggctcg gctccgggcg
    23401 caccagttcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    23461 gcgggtaatg ccgacatagg ccagccgccg ctcctcatca atattgtctt catcgatgct
    23521 gctctggtgg ggcaaaaacc cttcttccat accaaccatg tagacatagg gaaactccag
    23581 ccctttcgac gcgtgcagag tcatcagttg cacctgatcc agttcttcct cgctctcgcc
    23641 gcgctccatc atgtcgcgca gagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    23701 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    23761 catgcgcatt tcggcagctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    23821 catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    23881 ttctgccagc cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    23941 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccattc
    24001 acctagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag
    24061 aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    24121 ttcaggacga gagaaaaacg acgtaccgcc agatattttg tacgggatgc ggttttgcat
    24181 caggaatttt tcaaataccc gcgactgatg gttgccgcga taaagaatgg cgtaatcttt
    24241 gtactgcgtt ttattgacga agtgatgggc gatcagctcg ccggtgacgc gctcggcttc
    24301 atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa
    24361 cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    24421 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    24481 gctcagcagc accagattct gcggacgtgc gccgcgccag gagtagatcg actggtcatc
    24541 gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg
    24601 gctggtgttg gtgtcctgat actcatccac cagcaggtag cgaattttgt tctgccagcg
    24661 ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa
    24721 gtcgagaacg ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    24781 acggtcccgc tcgccctttg cctctgccgc cgcctgcgcc ggtgttttga gatcgttctt
    24841 ccagttggag atggtcgaaa ttagctgttg caggagaact ttgtcatctt caatcagccc
    24901 ctcggtcaac tctttaagca aagcaagctg atcggtatcg tcaaacagcg aaaagttagc
    24961 cttcatcccc agcgccgcat actcgcgttt gatgatatcc agccccagcg tgtggaaggt
    25021 ggaaatcatc agcccacgcg cctctttgcg ccccagcgtc tgcccgacac gctctttcat
    25081 ctcgcgtgcc gccttattgg taaaggtcac agccgcgata tgtcgcgcct gataaccgca
    25141 accgcggatc agatgggcga ttttattggt gataacacga gttttaccgg aacccgcgcc
    25201 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    25261 acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag
    25321 ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata
    25381 tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagatcaag aatggtgccg
    25441 atttcggcaa gctggcgaag aaacactcca tttgcccgtc aggcaaacgc ggcggtgatt
    25501 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagtggtg ttttcttgcc
    25561 cggtgctgga accaaccgga ccgctgcata cccagttcgg ttaccacatc attaaggtgc
    25621 tataccgtaa ttaacagcaa ggccttctcc gggagaaggc cttgaactct gaatcgctat
    25681 taacccgcaa cagcaatgcg tttcatatct gtcatatagc cgcgcagttt cttacctacc
    25741 tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc
    25801 gcaccttccg gaatagcttt acccaggtcg cccggttgca gctctgccat aaacggtttc
    25861 agcagtggca cacaagcgta agagaacaga tagttaccgt actcggcagt atcggagata
    25921 accacgttca tttcgtacag acgcttacgg gcgatggtgt tggcaatcag cggcagttcg
    25981 tgcagtgatt catagtaagc agattcttcg atgatgccag aatcaaccat ggtttcaaac
    26041 gccagttcaa cgcctgcttt taccatcgcg atcatcagta cgcctttatc gaagtactcc
    26101 tgttcgccga ttttgccttc atactgcggc gcggtttcga atgcggtttt gccagtctct
    26161 tcacgccagg tcagcagttt cttatcgtcg ttggcccagt cagccatcat gccggaggag
    26221 aattcgccgg agatgatgtc gtccatatgt ttctggaaca gcggcgccat gatctctttc
    26281 agttgctcag agagcgcgta agcacgcagt ttcgccgggt tagaaagacg gtccatcatc
    26341 agggtgatgc cgccctgttt cagcgcttcg gtgatggttt cccaaccgaa ctgaatcagt
    26401 ttttctgcgt atgccggatc ggtgccttct tccaccagct tgtcgaagca cagcagagag
    26461 ccagcctgca acataccgca caggatggtt tgctcgccca tcaggtcaga tttcacttcc
    26521 gcaacgaaag aggattccag aacgcccgca cggtgaccgc cggttgcagc cgcccaggct
    26581 ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc
    26641 ggtacgccga atccacgttt gtactcttca cgtacttcgg tgccagggca tttcggcgca
    26701 accattacga cggtgatgtc tttacggatc tgctcaccca cttctacgat attgaaacca
    26761 tgagagtagc ccagcgccgc gccgtctttc atcagcggct gaacggaacg cactacgtca
    26821 gagtgctgct tgtctggcgt caggttaacc accagatccg cctgcgggat cagttcttcg
    26881 taagtgccaa ctttgaagcc gttttcggtt gctttacgcc aggatgcgcg cttctcggca
    26941 atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat gttcaggccc
    27001 tggttcagac cctgtgcacc acagccgacg atgactactt ttttaccctg aagatagctc
    27061 gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccagctgc
    27121 tggcgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt
    27181 cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg
    27241 gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat taagggctgc
    27301 gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg
    27361 ccgcagcgcg cgggcaatgc acgttagccc gtccacgctc tctcggcaga ttcagcgcct
    27421 ggaagacgat ctcggtcagc cgctgtttgt gcgcgataac cgcacggtaa cgctaaccga
    27481 agcaggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc aacagctgcg
    27541 ccacaccatc gatcagcaag gaccgtcgct ctctggcgaa ttacatatct tctgctcggt
    27601 gaccgctgcc tacagccatc tgccgccgat tctcgatcgc ttccgcgcgg aacacccgtc
    27661 ggtggagatt aaactcacga ctggtgatgc ggcagatgcg atggaaaagg tcgtcactgg
    27721 cgaagcggat ctggcgattg ccgggaaacc ggagactctg cccggcgcag tggcgttttc
    27781 gatgctggag aatctggcag tagtgctgat tgcccccgcg ctaccctgcc cggtgcgtaa
    27841 ccaagtttcg gcagataagc cggactggtc tacagtgccg tttatcatgg ccgatcaggg
    27901 accagtacgc cggcgcattg agttgtggtt tcgacgcaac aaaatcagca acccgatgat
    27961 ttacgccacg gtcggcgggc atgaagcgat ggtgtcgatg gtggcgctcg gctgtggcgt
    28021 agccttgttg ccggaagtgg tgctggaaaa cagcccggaa ccggtgcgta accgcgtgat
    28081 gattttagag cgcagtgatg agaaaacgcc gtttgaactg ggcgtgtgtg tgcagaaaaa
    28141 gcggctacat gagccgctga ttgaggcatt ctggaagatt ttgccgaacc attaacccgc
    28201 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtaac ccaactcatt
    28261 caaccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc
    28321 gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg tacccagcgt
    28381 gttgaggaag cgcaacagcg cgcccggcga ttccgggaat tcgaagctgt agaggcgttc
    28441 ctgcaacgga tgcgacggac gcccgccgac catatagcgc acatgcagct tcgccatttc
    28501 gtcgtcggag agatccacta cgctgtagcc gccatcgttg agcatctgca aaatttcttt
    28561 gcgctcttcg aggccacggc tcaggcgcac accgacaaag atgcaggcgt ttttggcatc
    28621 ggcaaaacgg tagttgaact cggtgaccga gcgcccgcca agcagctggc agaacttcag
    28681 gaagctgccc ttctcttccg ggatggttac cgccagcaac gcttcacgct gttcgcccag
    28741 ttcgcaacgt tccgagacgt agcgcaggcc gtgaaagttc acgttagcac cagaaagaat
    28801 atgcgccagc cgctcaccgc gaatgttgtg ctgggcgata tattttttca ttcccgccag
    28861 tgccagcgcg ccagagggtt ccgctaccgc gcgcacatct tcgaacaggt ctttcatcgc
    28921 cgcacatatg gcatcgctat cgacggtgat gatgtcgtcg agatactcct ggcataaacg
    28981 gaaggtttcg tcgccgatgc gtttcaccgc aacaccttcg gcaaacagcc ccacgcgtgg
    29041 cagatcaacc ggatgacctg catccagcgc cgctttcagg caggcggagt cttccgcttc
    29101 cacggcaatc actttgattt gcggcatcag ttgtttgatc agtaccgcca cgcccgccgc
    29161 cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct gctggagcag
    29221 ttccagcgcc agcgtgcctt gcccggcgat caccatcgga tggtcgaacg gcggcaccca
    29281 ggtgaagccc tgctgctgtg acagttcgat cgctttcgct tttgcttcat caaagttcgc
    29341 gccgtgaaga agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga tatcggcggt
    29401 ggcggttggc atgacgatca gtgccttcac gcctaaccgt gcggaagaaa acgcgacgcc
    29461 ctgcgcgtgg ttacccgcag aagcggtgat cacgccgtgc gctttctgtt cttccgtcag
    29521 gcccgccatc atggcgtatg cgccgcgcag cttaaagcta tgaactggct gacggtcttc
    29581 gcgtttcacc agaatcacgt tatcgagacg cgacgagagt ttttccattt tttgtagcgg
    29641 cgtgacctgc gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat attcggcgcc
    29701 ttccggggcg ccggagagag gttgtgagtc tgccatcatt aaccccccag tttcgattta
    29761 tcacgcaccg cgcctttgtc ggcgctggtt gccaggctgg cgtaggcacg cagcgcaaag
    29821 gaaacctgac gttcacgatt tttcggtgtc caggctttgt tgccacgagc ttcctgcgct
    29881 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcggaatg
    29941 tcgatagcga tcaagtcacc atcttcaatc aggccaatgc tgccgccact tgccgcttcc
    30001 ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc gtcggtgatc
    30061 agcgcacagg ctttaccgag acccattgat ttcaggaaac tggttgggta gagcatttcc
    30121 tgcatccccg gaccgccttt cgggccttca tagcgaatta ctaccacatc tccggcgaca
    30181 actttaccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtagac tttcgccggg
    30241 ccagtgaatt tgaggatgct gtcatcgacg cctgcggttt taacgatgca gccgttttct
    30301 gcgaaattac cgtagagtac cgccaggccg ccgtctttgc tgtaagcgtg ttccagcgag
    30361 cggatacagc cattggagcg atcgtcgtcc agcgtatccc agcggcaatc ctgcgagaat
    30421 gcctgtgtag tacgaatgcc cgccgggcca gcgcggaaca tattttttac cgcatcatcc
    30481 tgggtcacaa tgatgtcgta ttgttccagc gtttgcggca acgtcaggcc aagtacattt
    30541 ttcacatcac ggttcagtaa ccccgcacga tccagctcgc cgagaatacc gataacacca
    30601 ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcgac tttacacagc
    30661 tgcggtactt tgcgggagag cttatcgata tcactcatgg tgaagtcgat ttccgcttcc
    30721 tgcgccgccg ccagcaggtg aagaacggtg ttggtagagc cgcccatcgc gatatccagc
    30781 gtcatggcgt tttcaaacgc cgccttactg gcgatgttac gcggcagtgc gctttcatca
    30841 tcctgttcgt agtaacgttt ggtcaattca acaatgcgtt taccagcatt gaggaacagt
    30901 tgcttgcgat cagcgtgggt tgccagcagc gagccgttgc ccggctgcga caggcccagc
    30961 gcttcggtca ggcagttcat tgagttagca gtaaacatcc cggagcagga accgcaggtt
    31021 gggcaggcgg atcgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcgccc
    31081 tggatcatcg catcaaccag atcgagctta atgatctgat cggacagttt ggttttcccg
    31141 gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc
    31201 agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtgg
    31261 gcgttgacca tatattcaac ggaatcagcg atcagttcgc gagatggcag tgaataaagc
    31321 atgcccccgt ggcccatagc gatcccatca tccaccgcaa tggtgttgaa ctctttggca
    31381 acgccgcccg ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatgaacg
    31441 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt accgaaatcg
    31501 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    31561 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    31621 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    31681 ccatttatct tcggtctcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt
    31741 aaccgggccg caacggcctt caccaacctg aataccatct acactgcgca ctggcgtgat
    31801 ttctgccgcc gtaccggaca taaagacttc atccgccagg tacagggatt cgcgcgacag
    31861 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt
    31921 aatacccggc agcgcggagg aggtgaacgg tggagtaaac agcacaccgt ctttcacttc
    31981 aaacaggttt tcgcctgcgc cttcagagat ataaccattc acatccaacg cgataccttc
    32041 ctgataaccg tggcggcgcg cttcgctacc caccaacagg gaagagaggt agttaccacc
    32101 agctttcgct gcggttggga tggtgtttgg tgcagcacgg ttccaggagg aaaccatcgc
    32161 atcgatcccc tgctccagcg cttctgcgcc cagatacgcc ccccacggga aagcggcgat
    32221 aatcacatcg gttgagtatc ccgctggcgg gtttacgccc atgccaacat caccgacgaa
    32281 aatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cgcgacaagc
    32341 ttccatcagc tcatcaatgc tctgcgaaac cgggaaacga tagattttgg cggagtcatg
    32401 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg agtcgtagca
    32461 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    32521 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    32581 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    32641 agatagcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    32701 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcagcca
    32761 tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    32821 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    32881 catttcagaa ttactggcac caggcggcac cagcggccag acgttctcaa gttcgtcgat
    32941 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    33001 ctggtcttta tgggtgatgt gttggccagg gatgccgaag gcgctggcta acatgaggaa
    33061 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tggaaaaaca gttgctgcca
    33121 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    33181 gcgttttacg gtgcccagct cttgcacatt catcataaaa gagccgtcac cggagataca
    33241 gacgacagta tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta aaccgaaacc
    33301 catggtgcct aagccgctgg aggtgatgaa gttttccggg cgagtgtgga cgatgtgctg
    33361 ggcagcccac atctggtgct gccccacatc tgtggtcacg atgcaatccg caggtttacg
    33421 atccgacagt tgttttaaca acaacggcgc gtagatagcg tcaccaggat ggtcgtaacg
    33481 ccaggcatgt tcatcacgca gttgcgcgca gtgttgctgc cagtcatcga tatttaacgg
    33541 ctgctgtaat gctggtaaca gagcatttaa atcaccctgt aatgccacat gtgcctgacg
    33601 cagcttgttc atttctgccg gatcgatatc catatggata acgctggcgt gtggtgcgaa
    33661 ggtgttcagt ttgccggtca cccggtcatc aaaacgtgcg cccacggcta tcagtagatc
    33721 gcactcctgc actgcgaagt tcgccgcttt ggtgccgtgc atccccagca tgcccagata
    33781 gtacggataa tctgcttcta ctgcgcccag ccctttcaac gtacaggtgg caggcatttt
    33841 tgtggcagcg agaaattcac gcaaagccga aactgcctgc gccatgccca cgccaccgcc
    33901 aacgtacagc atcggttttt gcgcttttgc cagcatctga cgcgcttgct cgacttcggc
    33961 atgtgggaaa gtcacttcgt tttcaacagt ggtgaaccac ggttccagat cgccgctggc
    34021 taactggata tcttttggaa tatcgaccaa aaccggacca ggacgaccag agctggcgac
    34081 gtcaaacgct tcggccatga tacgcggtaa ctcttccagt gattgcacca ggaaactgtg
    34141 cttggtgcag gctaacgaca accccagaat atccacctcc tgaaatgcgt cagtgccgat
    34201 aaacggtgcg gatacctgac cggtgatagc aacaacgggg atggaatcta acagcgcgtc
    34261 cgcaagcccg gttatcaggt tggttgcacc tgggccagac gtggcgatgc aaacgccagt
    34321 tttgccggta gcacgggcat aaccgatagc cgccattgct gcaccttgct cgtgtcggca
    34381 cagcaagtgt tccacgccac cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    34441 cggataaccg aaaacggtgt ttacaccctg tgcccgcaac gcatgtacca cccactgtgc
    34501 accattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc
    34561 attgtctgct cctcagttat gtttttaagg tcaaaaaaaa cccccggacc tttcggtgcg
    34621 ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt gcagccccgc
    34681 atggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact cgtagaaggg
    34741 ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt taccatagat
    34801 tttgcgaata gcacaagata ttttttggcc gatttttatg atgtctttaa taatctcaag
    34861 agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa aaattcaatt tcagtatgaa
    34921 tgttgttccc ttcctcgtag ttcatccttt gcccgctgca tttttgcgag catcattcca
    34981 ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac
    35041 gaatttgcgg gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggctta
    35101 tgtcactgtc aattgttcat acccgcgcag ccctgggagt aaatgcgccc ccaatcactg
    35161 ttgaggtaca tatcagtaaa ggtctaccag gcttaacgat ggtgggctta ccagaaacaa
    35221 cggtaaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc
    35281 cggcgaaaaa aatcaccatc aaccttgctc cagccgatct gccaaaagaa gggggacgat
    35341 atgatttgcc tatcgctatt gcgttgctgg cggcctcaga acagcttaca gccaataagt
    35401 tagatgaata tgaattagtc ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg
    35461 gcgcaatctc cagtgcaact gaagctatta agtcgggcag aaaaattatc gtcgcgaaag
    35521 ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc
    35581 aggctgtctg cgcgtttctg gaaggtaagc acgctctcga acacccgaaa ccaactaatg
    35641 cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag caaggaaagc
    35701 gaggactgga aattaccgcc gctggcgggc acaacctttt actgattgga ccgccgggaa
    35761 caggtaaaac aatgctcgcc agccgtatta atggcctttt gccagattta agcaatgaag
    35821 aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat
    35881 ggcggcaacg cccgttccgc tcacctcatc acagtgcgtc gttaactgcg atggtgggcg
    35941 gtggtgcaat tccagggcca ggtgaaattt cgctggcgca taacggcgtg ctttttcttg
    36001 atgagctacc tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg
    36061 ggcagatcca tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccagcttg
    36121 tcgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacac
    36181 cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc
    36241 tctcactgga gatcccatta ccgcctcccg gcattttgag taaaacggta gtgccgggag
    36301 aaaacagcac caccgttaaa caacgtgtaa tggccgccag agagcgccaa tttaagcggc
    36361 agaataaact gaacgcctgg ctggataatc cggaaatacg ccaattctgc aagcttgaga
    36421 gcgaagatgc gcagtggctg gaagaaacgc tgatccatct ggggttatcg attcgtgcct
    36481 ggcagcggtt attgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca
    36541 cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc
    36601 tgcaaaaact actgacataa aaaaagggca tttcgccctt tttattaatc gtcagaatcg
    36661 gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt
    36721 ggacgcttga tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggttca
    36781 cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg
    36841 ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg
    36901 gtgaagtcac catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta
    36961 gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg
    37021 gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca
    37081 aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc
    37141 cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccaatc agccgtgagc
    37201 tttcgaatca gacaactgga aaatcaactg ggtgtgaatc ttttcacccg ccacagaaac
    37261 aatatccgtt taaccgctgc cggagaaaag cttctgcctt atgcagaaac gctcatgagc
    37321 acctggcagg ccgcccgcaa ggaggtggcg catacctcac gacataatga gttttctatc
    37381 ggtgccagcg cctcgctgtg ggagtgtatg cttaatcagt ggctgggacg cttgtatcaa
    37441 aatcaggatg tccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg
    37501 gtaaaacagt tgcatgaacg ccagcttgat cttcttatta caactgaagc ccccaaaatg
    37561 gacgaatttt gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct
    37621 tctaaactaa agggagatct taactatctg cgactagagt gggggccaga ctttcaacag
    37681 catgaggcag gtttgatcgg tgctgacgaa gttcccattc tgacaaccag ttctgctgaa
    37741 ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt cagttgggca
    37801 cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat
    37861 gccatatggc tacaaaatag cgataaaaat gcgttgattc gcgatctatt gaaaattaac
    37921 gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact
    37981 tcggagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
    38041 aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg
    38101 aactacgccc ctaattaggg tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    38161 gacaggcagg tattctaacc gactgaact
//
LOCUS       DACADA010000028        37266 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.028, whole genome shotgun sequence.
ACCESSION   DACADA010000028 DACADA010000000
VERSION     DACADA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.028"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            163..1533
                     /gene="aroP"
                     /locus_tag="ISB17_001424"
     CDS             163..1533
                     /gene="aroP"
                     /locus_tag="ISB17_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAP1979370.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1576..2430)
                     /gene="ampE"
                     /locus_tag="ISB17_001425"
     CDS             complement(1576..2430)
                     /gene="ampE"
                     /locus_tag="ISB17_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAP1979371.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2427..2978)
                     /gene="ampD"
                     /locus_tag="ISB17_001426"
     CDS             complement(2427..2978)
                     /gene="ampD"
                     /locus_tag="ISB17_001426"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAP1979372.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3066..3959
                     /gene="nadC"
                     /locus_tag="ISB17_001427"
     CDS             3066..3959
                     /gene="nadC"
                     /locus_tag="ISB17_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAP1979373.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4163..4603
                     /gene="ppdD"
                     /locus_tag="ISB17_001428"
     CDS             4163..4603
                     /gene="ppdD"
                     /locus_tag="ISB17_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAP1979374.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4613..5998
                     /gene="gspE"
                     /locus_tag="ISB17_001429"
                     /pseudo
     CDS             4613..5998
                     /gene="gspE"
                     /locus_tag="ISB17_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            5988..7190
                     /gene="hofC"
                     /locus_tag="ISB17_001430"
     CDS             5988..7190
                     /gene="hofC"
                     /locus_tag="ISB17_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAP1979375.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7225..8268)
                     /gene="guaC"
                     /locus_tag="ISB17_001431"
     CDS             complement(7225..8268)
                     /gene="guaC"
                     /locus_tag="ISB17_001431"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP1979376.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            8493..9113
                     /gene="coaE"
                     /locus_tag="ISB17_001432"
     CDS             8493..9113
                     /gene="coaE"
                     /locus_tag="ISB17_001432"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP1979377.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9113..9856
                     /gene="zapD"
                     /locus_tag="ISB17_001433"
     CDS             9113..9856
                     /gene="zapD"
                     /locus_tag="ISB17_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAP1979378.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9866..10063
                     /gene="yacG"
                     /locus_tag="ISB17_001434"
     CDS             9866..10063
                     /gene="yacG"
                     /locus_tag="ISB17_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAP1979379.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10151..10549)
                     /gene="mutT"
                     /locus_tag="ISB17_001435"
     CDS             complement(10151..10549)
                     /gene="mutT"
                     /locus_tag="ISB17_001435"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAP1979380.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(10609..13314)
                     /gene="secA"
                     /locus_tag="ISB17_001436"
     CDS             complement(10609..13314)
                     /gene="secA"
                     /locus_tag="ISB17_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP1979381.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13376..13888)
                     /gene="secM"
                     /locus_tag="ISB17_001437"
     CDS             complement(13376..13888)
                     /gene="secM"
                     /locus_tag="ISB17_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAP1979382.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14118..15035)
                     /gene="lpxC"
                     /locus_tag="ISB17_001438"
     CDS             complement(14118..15035)
                     /gene="lpxC"
                     /locus_tag="ISB17_001438"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAP1979383.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15136..16287)
                     /gene="ftsZ"
                     /locus_tag="ISB17_001439"
     CDS             complement(15136..16287)
                     /gene="ftsZ"
                     /locus_tag="ISB17_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP1979384.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16348..17610)
                     /gene="ftsA"
                     /locus_tag="ISB17_001440"
     CDS             complement(16348..17610)
                     /gene="ftsA"
                     /locus_tag="ISB17_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP1979385.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17607..18437)
                     /gene="ftsQ"
                     /locus_tag="ISB17_001441"
     CDS             complement(17607..18437)
                     /gene="ftsQ"
                     /locus_tag="ISB17_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAP1979386.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18439..19359)
                     /locus_tag="ISB17_001442"
     CDS             complement(18439..19359)
                     /locus_tag="ISB17_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP1979387.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19352..20827)
                     /gene="murC"
                     /locus_tag="ISB17_001443"
     CDS             complement(19352..20827)
                     /gene="murC"
                     /locus_tag="ISB17_001443"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP1979388.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20881..21948)
                     /gene="murG"
                     /locus_tag="ISB17_001444"
     CDS             complement(20881..21948)
                     /gene="murG"
                     /locus_tag="ISB17_001444"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP1979389.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21945..23189)
                     /gene="ftsW"
                     /locus_tag="ISB17_001445"
     CDS             complement(21945..23189)
                     /gene="ftsW"
                     /locus_tag="ISB17_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAP1979390.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23189..24505)
                     /gene="murD"
                     /locus_tag="ISB17_001446"
     CDS             complement(23189..24505)
                     /gene="murD"
                     /locus_tag="ISB17_001446"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP1979391.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24508..25590)
                     /gene="mraY"
                     /locus_tag="ISB17_001447"
     CDS             complement(24508..25590)
                     /gene="mraY"
                     /locus_tag="ISB17_001447"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP1979392.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25584..26942)
                     /gene="murF"
                     /locus_tag="ISB17_001448"
     CDS             complement(25584..26942)
                     /gene="murF"
                     /locus_tag="ISB17_001448"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP1979393.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26939..28426)
                     /gene="murE"
                     /locus_tag="ISB17_001449"
     CDS             complement(26939..28426)
                     /gene="murE"
                     /locus_tag="ISB17_001449"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAP1979394.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28413..30179)
                     /locus_tag="ISB17_001450"
     CDS             complement(28413..30179)
                     /locus_tag="ISB17_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAP1979395.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30195..30560)
                     /gene="ftsL"
                     /locus_tag="ISB17_001451"
     CDS             complement(30195..30560)
                     /gene="ftsL"
                     /locus_tag="ISB17_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP1979396.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30557..31498)
                     /gene="rsmH"
                     /locus_tag="ISB17_001452"
     CDS             complement(30557..31498)
                     /gene="rsmH"
                     /locus_tag="ISB17_001452"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP1979397.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31500..31958)
                     /gene="mraZ"
                     /locus_tag="ISB17_001453"
     CDS             complement(31500..31958)
                     /gene="mraZ"
                     /locus_tag="ISB17_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP1979398.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32560..33564)
                     /gene="cra"
                     /locus_tag="ISB17_001454"
     CDS             complement(32560..33564)
                     /gene="cra"
                     /locus_tag="ISB17_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAP1979399.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33646..33732)
                     /locus_tag="ISB17_001455"
     CDS             complement(33646..33732)
                     /locus_tag="ISB17_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAP1979400.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33744..34235)
                     /gene="ilvN"
                     /locus_tag="ISB17_001456"
     CDS             complement(33744..34235)
                     /gene="ilvN"
                     /locus_tag="ISB17_001456"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP1979401.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34238..35962)
                     /gene="ilvI"
                     /locus_tag="ISB17_001457"
     CDS             complement(34238..35962)
                     /gene="ilvI"
                     /locus_tag="ISB17_001457"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAP1979402.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEIGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGARFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36280..37224)
                     /gene="leuO"
                     /locus_tag="ISB17_001458"
     CDS             complement(36280..37224)
                     /gene="leuO"
                     /locus_tag="ISB17_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAP1979403.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9001 a        10399 c         9072 g         8794 t
ORIGIN      
        1 gtgcattctc gcgcgtatac cattatcttg tgatagttga aagtactatc atcataattc
       61 agttacataa aatgtaaaga aaccaataca acaaacggaa ttacaaaatt acacacgcat
      121 tgctgcgtag ttcaaaaaaa caacacaaca cgaggtttca taatggaagg tcaacagcac
      181 ggcgagcagc taaagcgcgg ccttaaaaac cgccatattc agcttatcgc gctgggtggc
      241 gcgataggga cagggctatt cctgggtagc gcctccgtaa tacagtccgc agggccaggg
      301 attatcctgg gttacgccat tgctggtttt atcgcctttc tgatcatgcg tcagctaggt
      361 gaaatggtgg tcgaagaacc tgtcgcaggc tcctttagcc actttgctta taaatactgg
      421 ggcagttttg ccggtttcgc ctctggctgg aactactggg tactgtacgt tttagttgcc
      481 atggcagagc tgactgccgt gggtaaatac attcagttct ggtatccgga aatcccaacc
      541 tgggtttctg ccgccgtgtt ctttgtggtg atcaacgcca tcaacctgac aaacgtaaaa
      601 gtgtttggcg agatggagtt ctggtttgcc attatcaaag ttatcgcggt ggtagcgatg
      661 atcatcttcg gcgcctggct gctattcagc ggcaacggcg gcccgcaggc gagcgttagc
      721 aacctgtggg atcagggtgg tttcctgcca cacggcttca ccggtctggt gatgatgatg
      781 gcgattatca tgttctcttt cggtggtctg gaactggtgg ggatcaccgc agcagaagct
      841 gataacccgg agcaaagtat cccgaaagca actaaccagg ttatctaccg catcctgatt
      901 ttctatattg gctcgttagc cgttctgctc tcactaatgc cgtggacccg cgttaccgcc
      961 gataccagtc cgtttgtgct gatcttccac gagttaggcg atacctttgt agcgaatgcg
     1021 ctgaatatcg tggtactgac tgctgcgctc tccgtgtaca acagctgcgt atattgcaac
     1081 agccgtatgc tgttcggtct ggcacaacaa gggaacgcgc caaaagcgct ggcgtctgtc
     1141 gataaacgcg gcgtaccggt taacactatt ctggtgtctg cgctggttac ggcattgtgc
     1201 gtactgatta actaccttgc cccagagtcc gctttcggac tgttaatggc gctggtggta
     1261 tctgcactgg tcatcaactg ggcgatgatc agtctggcgc atatgaaatt ccgtcgtgcc
     1321 aaacaagaac aaggcgtggt aactcgcttc cctgccctgc tttatccgct gggtaactgg
     1381 atctgcctgc tgtttatggc ggcggtactg gtgattatgc tgatgacccc aggaatggcg
     1441 atttcggtat acctgatccc ggtatggctg atcgtgttag gtatcggcta tctgtttaaa
     1501 gagaaaaccg ccaaagccgt aaaagcgcat taatctctct acgccctcac ccgtgcaggg
     1561 tgagggcaat aatctttaca ccaacgcccc gtaaatcgtc agtagcgcaa ttaccaccac
     1621 gaccacgaac gaggttttct tcgccattga aaccgcggcc tttggcgtct ccaccttatc
     1681 gacatgcggt tcacgcgcca gagagaactg tgccagacgc gttaatacct gatattgcga
     1741 agtatggaaa tcacccagcg aagcaaacca ggccggtaac gctttctcac catgaccgat
     1801 caaggcatat accacacccg caagacgaac cggcacccaa tccagtgcat gaagcacggc
     1861 atcaatgccg gactgtaaac gatgatgcgg cgtctggtat cgcgccagcc agtattgcca
     1921 tgcacgcaaa aacgcatacc ccatcagcgt aacgggtccc caggttcccc ccacaatcag
     1981 ccagaacagc ggagcaagat aaaaacgaaa gttaatccac agcaatgcat tttgcagctc
     2041 acgcaaatac tcacgttcat cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc
     2101 agccatcgtg gcatgggcat ggctatcatt acgtgaagca gctgtcagat aagcatgata
     2161 atgaagacga actttacctg cgccaataca cagcaaacca atcagcagcc acaccagcaa
     2221 cgtgggaacg ttgaacaata caccctgcaa tgcgcgtaac agtaaaaaag tcacgcccat
     2281 cgcaataatg gtcatgcata acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa
     2341 cgcttcaaga cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat
     2401 taacaccagt aaggttgtaa atagcgtcat gttgtctcct tgctgaccag cgcacgaaac
     2461 cgtgcccaat caaatgcagg accgggatcg gttttccgat ccggcgcaat atcacaatgg
     2521 cccgtcatgt tattagcgat atccggatag cgatcaatca gtgcccgtgt aaccgccgca
     2581 agctgttgat actgcgcatc ggtatacgcc agcgtatcgg tgccttccag ctcaatccca
     2641 atagaaaaat cattgcagcg ttcgcgcccc tgatactgag agactcccgc atgccaggct
     2701 cgtttatcga aaggaacata ctggactatt tcaccatcac ggcgaatcaa acagtgagcg
     2761 gagacgcgca aatgggcgat ctccgcaaag aaaggatgcg cctgcggatc aatagttcca
     2821 gtgaataatg cgtcgatcca cggaccgcca aactcgcctg gcggcaggct aatattgtgc
     2881 accaccagca gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg
     2941 cggcgcgcgc cagccagcca cccctgttct aacaacatgc aggatctcct tatatgtggt
     3001 gctaataccc ggttcagagt agcatgtttc taccttatga ttcgttagct atctggagtt
     3061 ttaacatgcc gcctcgccgc tataaccctg acacccgacg tgacgagctg ctggaacgca
     3121 ttaatctcga tatccccggc gcggtggccc aggcgctgcg ggaagattta ggcggaacag
     3181 tcgatgccaa caatgacatt acggcaaaac ttttaccgga aaactctcgc tctcatgcca
     3241 cagtgatcac ccgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa gaggtgttta
     3301 ttcaactggc aggcgacgat gtcaccataa tctggcatgt ggatgacggc gatgtcatca
     3361 acgccaatca acccttgttc gaacttgaag gcccatctcg cgtgctgtta acgggcgaac
     3421 gcactgcgct taattttgtg caaacccttt caggagttgc cagtaaggta cgccactatg
     3481 tcgaattact ggaaggcacc aacacgcagc tgttggatac gcgcaaaacc ttacccggcc
     3541 tgcgttcagc tctgaaatac gcggtacttt gcggcggcgg agcgaatcac cgtctggggc
     3601 tttctgatgc cttcctgatc aaagaaaacc atattattgc ctccggctca gtgcggcagg
     3661 cggtcgaaaa agcgtcctgg ttgcacccgg atgcgccagt agaagtcgaa gtagagaatc
     3721 tggaagaact tgatgaagcc ctgaaagcag gtgccgatat catcatgctg gataacttcg
     3781 aaacagaaca gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt
     3841 ctggcaacgt cactgacaaa acactgcgcg aatttgccga aacgggcgtg gactttatct
     3901 ccgttggtgc actaactaaa cacgtacagg cactcgacct ttcaatgcgt tttcgctaat
     3961 tgctaacaga tttatccgct ccatcgcggg cggataaatc tccccttccc caactttctt
     4021 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa gtaaaaaacc tcggaagctg
     4081 ctttcccgtt tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca
     4141 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta ttgaactgat
     4201 ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta
     4261 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
     4321 agagttgtgc gcgctggaac atggtggatt aaatacctgc gacggtggca gcaatggcat
     4381 tccttcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
     4441 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacaccgg gttgggataa
     4501 cgcaaacggc gtcaccggct ggacgcgtaa ctgcaatatt caaagtgaca gcgcattgca
     4561 gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
     4621 tccacagctc actgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga
     4681 ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ctactggacg cattgcattt
     4741 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
     4801 cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga
     4861 gttgctgact cgaacgttac aatctgcgct agaacaacgc gcgtctgata ttcatatcga
     4921 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
     4981 ggacgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct
     5041 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
     5101 cgccgtctca tttcgtattg cgaccttagc atgtcggggt ggtgaaaagg tggtattaag
     5161 gttgttacag caggtgaacc aggcactgga tgttaacacg cttggaatgc agccgtcaca
     5221 actggtggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
     5281 tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaacgctga ataccgctga
     5341 cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggat taaaccagac
     5401 gcaaatccat tcgcgtgccg gactcacctt tcagggcgtt ttgcgtgcgt tattgcgcca
     5461 ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat
     5521 aaaagccgcg caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga
     5581 aacgctggta cgtttacagc aaatgggagt cgcccgctgg atgctatcat cggcgcttac
     5641 gctggtaata gcccagcgtc tggtacgcaa actttgcccg cattgtcgcc ggcagcaagg
     5701 ggagcccatc catattccag acaatgtatg gccgtcgccg ctgccccact gacaggcacc
     5761 cggttgtgta cattgctacc acggttttta tggtcgcacg gccttatttg aagttctgcc
     5821 cataacaccg gtcatacgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac
     5881 gcacgcccga caggcgggta tgcgaacgct ttttgaaaac ggctgcctgg ctgtggagca
     5941 aggcttaacc acctttgaag agttaatccg cgtattgggg atgccgcatg gcgagtaagc
     6001 aactctggcg ctggcgtggc ataaccggcg acggcaatgc gcaagatggg atgctatggg
     6061 cagagagccg tgctttgctg ctcatggcac tacagcaaca gatggttacc ccactaagcc
     6121 tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc
     6181 atcaactggc gacgctactc aaagccgggt taacgctttc tgaagggctg gctctgctgg
     6241 cggaacagca tcccagtaag cagtggcaag cgttgctgca atcactggcg cacgatcttg
     6301 aacagggcgt tgccttttcc aatgccttat taccctggtc agaggtattt ccgccgctct
     6361 atcaggcgat gatccgcaca ggtgaactga ccggtaagct ggatgaatgc tgctttgaac
     6421 tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt
     6481 atcccattat cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc
     6541 tgccggagtt tgccgctatc tataagacct ttaacactcc gctaccggcg ctaacgcagg
     6601 ggatcatgac gctggcaagt tttagtggcg aatggggttg gttgctggtg ctattcggct
     6661 ttctgctggc gatagccaat aagctgctga tgcgccaccc aaccaggctt atcgcacggc
     6721 agaaattact gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc
     6781 agatctttac gattctgacg ctgacacaaa gtgcaggcat ttctttttta cagggcgttg
     6841 agagcgtcag agaaacaatg cgctgtccgt actgggtgca acttctgaca caaatccagc
     6901 acgatatcag taacggtcac cccatctggc tggcgctaaa aaatgccggt gagtttagcc
     6961 cgctctgttt gcaattagtg agaacaggag aggcatccgg ctcgctggat ctcatgttag
     7021 acaacctcgc ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct
     7081 tactggaacc ggcgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcaa
     7141 tgtatctgcc aattttccat ttaggcgatg cgatgagtgg aatgggataa cgctggcgtg
     7201 ttgctccacg ccagcgttgg tatattacag gttgttgaag atgcggtttt cttgttcctg
     7261 cacacggata aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt
     7321 acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag
     7381 cggcagctta acggttttac cttctgctgc gcgatattcc gcaacaccgc caacgtgacg
     7441 tttcatcgca gactctgagc tcatgccgta gaacagcata aatttctcgc cgttctcctc
     7501 aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc
     7561 ggcaccgccg ccaaaggctt tcgcgacatc gcccggcgtg gtgcagccac cgtcgctgac
     7621 gatcattccg cccagaccat gcgcagcatc ggcacattcg atcaccgcag aaagttgcgg
     7681 ataaccgacg cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt
     7741 aacgatatca gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc
     7801 agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg
     7861 ttcggaataa ccattcgcca cgtcaataca aacgaagttt aatgccgggt tcaggtcgag
     7921 aatctgttta gttttttcga aatccgcatc agacgtgccg gtagaaacca tcacatgttt
     7981 cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt
     8041 atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtgcctac
     8101 ggtgtccata ttggcggcga taattggcac gccggaccag cgctgacctg aatgtttgaa
     8161 ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg
     8221 gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct
     8281 ggggttaatg gctataaggg taagagcgca actccagtga cgttatcata cgcactaatc
     8341 aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg
     8401 gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cgctcatttt taacgttttg
     8461 ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag
     8521 gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca
     8581 ttgatgccga tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca
     8641 ttgctgatca ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct
     8701 tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc
     8761 cgctgattca gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactat
     8821 gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg
     8881 tggatgtcag cccagaaacg caacttaagc gcaccatgca gcgcgacgat gtaactcgcg
     8941 agcatgtcga acaaatcctt gctgctcagg caacgcgcga agctcgcctt gccgtggcag
     9001 atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc
     9061 acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac
     9121 ccaggtcctt tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt
     9181 tttgattcag caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt
     9241 cttccgtaat gtcagcgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct
     9301 gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg tgcccggcgt
     9361 ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat
     9421 ttccgcgccg cgtatcgggc aatttctacg tgaagatcgt ttgattgctc tggtgcgtca
     9481 gcgactgagc atcccgggcg gctgttgcag ctttgattta cctacattgc acatttggct
     9541 gcatctaccc caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc
     9601 acttacccag gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa
     9661 acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct
     9721 gaatctgtcg ctcgattcac agctttatcc gcaaatttcc ggtcataaga gccgttttgc
     9781 cattcgtttt atgccgctgg acagtgaaaa cggacaagta ccagaacgtc tggatttcga
     9841 actggcctgt tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg
     9901 cgggaaaacg gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg
     9961 tcagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga
    10021 tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacattcta tctgatgcga
    10081 tgctggcctg ataaaacgcg ccagcgtcgc atcaggcatc atatgcaaat gtcggatgca
    10141 gcgtgacgcc ttattcgaca tacacacgct taagtttcgc aattaccggt tcattggctg
    10201 gcggaaaatc atcggcatta agaccgacca gcgacatcca ctcaccgggt tgcccttctt
    10261 taccccacgg ctccccttcc cagctttcga ccagccagaa ccacagagtt atatgcctgt
    10321 ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgactt
    10381 cttcctgaag ttcacgtacc actgcctgtt ccggcgtttc gcccatttca attttaccgc
    10441 cggaaaactc cagtttattc gccatgtgcg catctgctgc gcgacgcgtt ataaagattt
    10501 cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt gtcttaaacc
    10561 tataaaaaag gcgcagaatc ctgcgccttt tacttcaaca gttagctttt attgcaggcg
    10621 gccatggcac tgcttgtatt ttttaccaga accacacggg caaggatcgt tacgtcctac
    10681 ttttcgttca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    10741 gctaagctgc tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    10801 ctcaacctct tcaggcatac gcacctgaac tttgctcagc gtgctgataa cttcatattt
    10861 taacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    10921 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgcagccag
    10981 gtgctctttc cacagagagt caagcgtttg cagcatgacg cctttctcga agtgacgcat
    11041 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    11101 gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cggcaattgg
    11161 caaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    11221 ttccagcgac tgtggcggaa tgtaggcatc aatggtcgct ttgaacacat cttcacgaat
    11281 gctgttgatg gtttcgctca catcgctgac atccagcagt tcgttacgct gggagtaaat
    11341 ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    11401 gttacggctt tcaactttac gctgggcgtt ggcaatcgct ttggtcaccc acgggtgctc
    11461 aatggcttcg cctggcttca tacctagttt acgcatcatg ccggatactc ggtcggaggc
    11521 aaaaatacgc atcagcgcat cttccatcga cagatagaaa cgggaagaac cagcatcccc
    11581 ctgacgacca gaacgaccgc gcagctggtt atcgatacga cgggattcgt gacgttcagt
    11641 accgatgata tgcaggccac ctgctgccag taccgcatcg tgacgtacct gccagtcggc
    11701 tttaattttt tcaatttgct ctgcagtcgg attttccagc gcggcaactt ctgcctgcca
    11761 gctaccaccg agcacaatat cggtaccacg acccgccatg ttggtcgcga tagtcaccgc
    11821 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcatggaatt tggcgttcag
    11881 gacgttgtgc ttaataccgg ctttggtcag ttcatttgac accagctccg atttttcgat
    11941 ggagattgta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    12001 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    12061 cattggacgg ttggtcggaa caacgacagt atccagctta tagatggagc tgaattcgaa
    12121 agcttcggta tcagcagtac cagtcatccc cgccagtttt tcatacagac ggaagtagtt
    12181 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    12241 ttccacagcc tggtgcagac catcagacca gcgacgcccc tgcatggtac gaccagtgtg
    12301 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac gggtaaacag
    12361 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    12421 cagagactcc ccttcatcca tgatgccttc tttcaccagc agttcttcaa tcagcaccag
    12481 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    12541 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    12601 gcgtttatac atttccgagc tgtcttctgc cgggccagaa atgatcagcg gtgtacgcgc
    12661 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    12721 acgttcttca ggactgaacg ccatgttgtc acgcaggtag tcaaagccgt attcgttgtt
    12781 cgtaccgtaa gtgatgtcag cagcgtaggc ttcacgcttt gccggtgctg gcatgcccgg
    12841 caggttgata ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    12901 acgttgcgcc aggtagtcgt tgacggtaac gacgtgcacg cctttaccgg ttagtgcgtt
    12961 caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttccgcgat
    13021 gcagcgttca ttaagaacca taccgccgag cagctgtacg tcgaagtggc gcatgccaaa
    13081 aacacgctta ctggcttcac gcaccacggc gaaggcttcc gggatcaagt tttccagcac
    13141 ttcgcctttt tccagacgcg cgcggaactc tgccgttttt cctttcagtt cttcgtcgga
    13201 gagtttttca atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    13261 cagggtgcga tcgttacgac taccgaaaac tttagttaat aatttgatta gcataataaa
    13321 atctcaaacg ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagc
    13381 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    13441 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    13501 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    13561 gcaaagattc ttcagcaacg ggcagcgttt gcggtgccat tgcaaaggaa agatggcgga
    13621 ttaccgtgcg aatggcatgt tgatgccagt aatcaacgga atagttcgaa ttcgggcggc
    13681 gcgtgttcgt ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    13741 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc attgctgagt gcaggcaaac
    13801 ctaaactcgc cgcgaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    13861 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgtat tcaaacgtat
    13921 ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa gataagttgt
    13981 gcgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagctctt ataaaattca
    14041 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    14101 cgacagtata aatgtcgtta tgccagtacg gctgaaggcg ctttgaaggc caacggcagt
    14161 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    14221 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    14281 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    14341 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    14401 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    14461 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    14521 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    14581 ccattgtacg gtttaaattc agcccactta tcgccatctt cgacacgaac agtctcttta
    14641 atgcgaacaa atttcttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    14701 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    14761 atgttatcga tgcccaagcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    14821 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    14881 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    14941 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    15001 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    15061 ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    15121 aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat ccagataatc
    15181 cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg gcttctgctc
    15241 ctgggtcagc ggagccatac cgtgctgctg gtagcgatcc atcactggct gctgaacctg
    15301 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    15361 agttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacggtcgc
    15421 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    15481 gtcgaagccc gctgtgatgt ttaccagcac gccgcgtgcg ccagacaggt cgatatcttc
    15541 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    15601 cacaccagaa cccatcattg cgtagcccat ttcagacatt acggtgcgta cgtctgcaaa
    15661 gtccacgttc atcaaacccg gacgagtaat cagttcggcg ataccttgca cagcgccttt
    15721 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccacggc ccagaacttt
    15781 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    15841 cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    15901 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    15961 accggtgcca ccacccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    16021 acgcagtgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    16081 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    16141 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc gctcacgcac
    16201 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    16261 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagg
    16321 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    16381 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    16441 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    16501 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    16561 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    16621 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    16681 ttcgttgacc agattgagca gctcggtata gcgtggctcg atcacctctg ccagggtctg
    16741 acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc tctcatcttt
    16801 tccaacgatg gagcccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcacttgg
    16861 cggtgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    16921 cttcgtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    16981 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    17041 cagaccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    17101 gtttttcgcc atatcattgt gacatgtgat caggtgcact tttgcctgca tccgcacgcc
    17161 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    17221 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    17281 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    17341 gcagctgatg tgcttaccag aaagcgccag atataccgaa gatatctgac aatctgccat
    17401 caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga ggtcgttcac
    17461 cccgccttta tccataccac gcgacgggca actacccacg ccaatgatat tgaccatacc
    17521 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac
    17581 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    17641 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    17701 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    17761 agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    17821 tcgttattca gcgtcaactg ccaggaacgt cgcgcggtca tcgccgcttc cttcagagta
    17881 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattggcg
    17941 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    18001 ctgaaggtgt ttccttccgc gtctaccata tgttgatcat tccaccgcgc aatcggcaca
    18061 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    18121 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg ggtcataaag
    18181 gtgcctggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    18241 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagccc
    18301 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    18361 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctatt tcgcgtgttc
    18421 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    18481 gctcatacct gcctgacgtg ccgccatcgg caccagactg tggctggtca tacccggtga
    18541 ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt caatacgtcc
    18601 ccatcctttg caacctaacg tcgtccatgc ttttagcact aatgcctgca aattggcctc
    18661 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    18721 ctcataatca tagaaggttc cggccggttg aatacgtatt gacggtaaaa tttcttcacc
    18781 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    18841 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    18901 tcccacactg gaaccttcgc ggctcggctt aacgattacc ggcaaaccca gagaagaaat
    18961 ttctgctaac tgcttatcgt taaggccttt ttcaaactct gcgcgggtta atgctaccca
    19021 cggcgcgacc ggtaaaccgg cactctgcca cagaagtttg ctgcgtagtt tatccattga
    19081 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    19141 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    19201 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg cgtcaatacc
    19261 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    19321 ggaggtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    19381 gcggcttcag tttgatttca gctaaagaac gagcaatttt tccgatatta ccagccccct
    19441 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgccg
    19501 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    19561 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    19621 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    19681 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcacgc gccgctttaa
    19741 tagtggcgtc cacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    19801 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcgaaacgg cgccccgtcc
    19861 cctggaagct ctccagcgca cgcaaaatag cctcgtcgtc aattccctct tccgtagcaa
    19921 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc
    19981 gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    20041 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc tgacgcccca
    20101 cacgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgcg cgaccgtaaa
    20161 acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag tcgccctggt
    20221 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    20281 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    20341 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    20401 ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt ccggcaatgg
    20461 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    20521 tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatcgcg ctggaaacaa
    20581 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgctccca
    20641 gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    20701 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg ccgacaaaat
    20761 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    20821 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    20881 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    20941 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    21001 gttcgcgaca gcatccacgc taagctgtga ctgctcgata attttggctg cgcccgcttt
    21061 ttccagcggt agcgcattcc agtattgctg acggtcttta tgttgaaacg gcacaaacaa
    21121 cgccggtagt cctgccgcgg cgatttcact caccgttaac gcaccggagc ggcaaacgac
    21181 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca ctttatgctg
    21241 cggttgcccc acttcggcat acgcctgttc aacggattgt tgcgaacctt tgccgctctg
    21301 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctgattaag
    21361 aatgcgtgcg ccctgggaac cacccactac caacacacga accggacctt cacgtccagc
    21421 caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt tacccaccac
    21481 ttccgcatta gggaaagcac ctggaaacgc ctgcatcact ttggtagcaa tcttcgccag
    21541 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    21601 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gcaccacgtc
    21661 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    21721 cgcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa tgaaatcaat
    21781 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    21841 aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agccccggga atacatgtcc
    21901 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    21961 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    22021 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa tgtcagacct
    22081 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctgaaa actaaaccag
    22141 ataccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa tgctttgcgg
    22201 ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag caccacaccg
    22261 acatatccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    22321 tactccagtt tttgtaccga gttacctaaa ccttgtcccc acagttcacc gcgaccaaac
    22381 gccatcaacg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cggattccag
    22441 aatgcggtaa cacggcggat acggtacggt tcggcgagga tcagcaacac aaccgctgaa
    22501 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    22561 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    22621 acagccaaca ccagaattac gcccatcggt ttcagaaagc cgcgcaggtt attacgtact
    22681 tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag ttttgtcagc
    22741 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaaccgag
    22801 ctacccacta ccagaacgat catcagcagg atgatagatc caagcagcat cgtggcactg
    22861 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat gaatgccagg
    22921 atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    22981 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc gaaggtcagc
    23041 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    23101 acccagccct ttagcgccgt ggagatccag accagaatac tgaatcctgg caggcgcggc
    23161 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    23221 cattgcctcg ttgttcaaag ttcttgaatt gatcaaggct ggcacaggcc ggggagagca
    23281 gaaccatatc gcccggctga acacgcggag caagcaagcg catcgcctgt tccatggttt
    23341 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtcc cgaccgaaac
    23401 aatacagacg tacattatcg ccattcaggt aacgcgccag tgggctaaag tccgccgatt
    23461 taccatcgcc acccagcaac aaatgcagcg tgccgtctac atgcaggcca ttcagcgccg
    23521 cttccgtact gccgacgttg gtcgcttttg aatcgttaac ccagcgcaca ccgttatgct
    23581 ccagcacaac ttcaaagcga tgcggcagac cagtgaatgt ggttaacgct ttcaggctgc
    23641 tggcacgcgg taaccctgcg gcatctgcca gcgccagcgc cgccagcgca ttggtgtagt
    23701 tatgctgccc ggaaagtttc atttctttca cgttcagcac tttctcgcct ttcacccgca
    23761 gccaggtttc gccctgctga tgattcaggt gatagtcgcc catgttgacg ccaaagctga
    23821 cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca ttaaccacgc
    23881 aaacttttgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg
    23941 gatagcgatc catatgatct tcagtcacgt tcagaatggt cgccgccacc gcttgtaagc
    24001 tggaagtggt ttccagctgg aaactcgaca gttccagcac gtacagttcg cactcatcat
    24061 ccaacagcat caacgcaggc aggccaatat taccacccac accaacgtta acccccgccg
    24121 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtgatcg
    24181 ccacaatcgg tgcttgtgct tcacgacaga acagctcgat atcgccaacg atttcgattc
    24241 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    24301 tcagatctgc cgccatcagc cattcatcat tcagaccgcc cgtgtggcgt tctacggctt
    24361 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    24421 cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg ataatgacga
    24481 catttttacc ctggtaatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    24541 gaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    24601 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    24661 ggagccgacc tgcaggatga cggaaagcgt ttctaccacg aacacgcccc ccataatcac
    24721 cagcaggaat tcctgacgca gcagtacggc gataatacct aacgcaccac ctaacgccag
    24781 cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    24841 ccctgccccg actatcgcgg tacagacaat gaccagttcc ccggcgtgtc gtagatacgg
    24901 tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc
    24961 accggcgaca aatacggtcg gcataattgc cagaccgtcg agaccatcgg tcaggtttac
    25021 cgcgttgcca gtacccacaa tgacaaagta agccagcaga atgtagaaca gccccagttg
    25081 cggcatcaca tctttaaaga acggaaccac caactgcgtt gcgggcgtgt ctttgccggc
    25141 aaggtacagc gcgaaggcaa cacccagtgc aatgaccgac atccagaaat acttccaacg
    25201 ggcaatcagg cccttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    25261 aacaccgtaa cctaccagca ccaccagtac gcaccagacg tacggattgg acgggtaagc
    25321 ccacagcagc acggagatca caatcgccgt caggatcatt atcccgccca tggtcggtgt
    25381 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac caaaggaaag
    25441 tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca gcgcggtcag
    25501 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    25561 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    25621 tcttccatgg cagcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    25681 atcagtgatt taagacgcgc gataagcgca gttttatcgg aaaaatgttc gccaacgccg
    25741 ctggcggtgc tgatagcatg gctttgttta cccacactta acacgcagtc aataccagca
    25801 gcttttgccg cttcgcccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    25861 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagaacctg aacagcggcg
    25921 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    25981 tttatgggga acagacggcc tggaacagct ttcagattta ccagccccgc tttgatagca
    26041 tcaagcgttg cgcccacaga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    26101 tgacgccccg gcaacggcag cagaacatcg acgctacctg taggggtttg cagggtaaat
    26161 tccgtaccgt gcgaggttac atggatattg gtggcggtga aatcgctgtt ggcggcatta
    26221 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcaa ccagtcgttg
    26281 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    26341 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    26401 accagcgccg cttccgggcg agtcagactc acagtccagg caatttcacc ctgatggttc
    26461 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    26521 acaccgatgt cgttattgag attgcctgcc gtataaagtg tgttgccgca ctggcttaaa
    26581 attgccgccg tcatctcttt aacggaggtt ttgccggagg aacctgtcag agcaaccacg
    26641 cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    26701 ttgacgatta actgcggcag atcaatatcc agcggacggc taaccagtag tgcgcctgcg
    26761 ccgccagctt tcgcctggtc ggcaaaatca tgagcatcga aacgttcgcc tttcagggca
    26821 acaaacaggc agcccggcgt cagttttcgc gtgtcagtgg ttacagcatc aagggtaata
    26881 tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt tacgctaatc
    26941 atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    27001 tgccaacaat ctggtagtcc tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    27061 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatatc
    27121 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgaggctct tcggtacgcg
    27181 ggttatcgtc cgtcaccacc accacgtcag caaactcttc agcaattgcg cccatcagtg
    27241 gacgtttgcc tttatcacga tcgccaccgc agccaaagac gcaccacagc ttgcccgcac
    27301 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    27361 ccaccaccgt cggtttgcct ggtgcagtga acacttccat acgtccgcaa accggttgca
    27421 gacgcgcggc ggttttcagc aaatcagcca gcggatagcc gagtgccagc agtgtcgcca
    27481 gcgccagcag caggttgctg acgttgaatg cgcccatcag gcgactttca atttccccat
    27541 cgccccaact cgagctaaag caaatggtcg caccgctgtc gtgatagttc acgtcggtcg
    27601 ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    27661 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta ataatcgcct
    27721 gaccgcaatg atgtgcagaa taaagcagcc atttcgcggc ttcgtagtgt tccatatcac
    27781 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca aatttcaatg
    27841 ccgccacacg gtgttgaacc agcccgtggg atgaaacttc cattgcgcaa aaagtcgcgc
    27901 cctgatccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg gttgtatttt
    27961 ctgtcgggat cactttcccc agcaggccgt taccaacggt gcccattacc gcgctggttt
    28021 cgccaagcag ttgactccac tgcgccaaca gctgggtagt cgtggttttg ccgttggtgc
    28081 ccgttacgcc cacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    28141 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    28201 tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    28261 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    28321 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    28381 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt accctcgcct
    28441 tgattaatca caaattcatt tttatcgcct gttgtcagcg catccggctc gatgttcatg
    28501 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    28561 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    28621 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccatc cggcccgacc
    28681 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    28741 ccgccgcccg gtagcgccac gctttccatc atatgcacca cggtgcgaac aatggattcc
    28801 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    28861 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    28921 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    28981 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagccc aaaacgtgag
    29041 taagtatcta ctaacgctga ggacggcatc gctaacgcca gtttggaaac accgacgtta
    29101 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    29161 tcgtggccgt taattcgata aggaacggta ttgagtaccg agttttcccg caccacgcca
    29221 cgttgcaacg cggtcatcac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    29281 gtgatggtac ggttacgcat cgcttctttc ggcgtgccgc tcagattatt tgggttgtat
    29341 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    29401 ccagattcag ccttgttaaa ggccactgcg ttgttcagtt cgcgataaac cagcgcctgc
    29461 aggcgttcat caatactcag cgtcaggttg tgcgctgcct ggctgtcagt agaagaaata
    29521 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    29581 agccatttat cgaaactctt ctcgaccccc tcaatccctt ggctatcgac gttagtaaag
    29641 ccgatgaggt gagcagtcac ttcgccggat ggataataac ggcgagattc ttcacgcaga
    29701 tgaattcccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt tacctgacgc
    29761 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgagag ctgatccagc
    29821 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    29881 tcatgcactt ctttcgggtc agcccaaatc gcttttactg gcacactcac cgctaacggg
    29941 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    30001 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    30061 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    30121 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    30181 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    30241 atttgcagct tttccgtggc tatcctttcc acccggctat ggtcgccgag cgcattctct
    30301 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    30361 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa
    30421 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    30481 acgccaggca aggcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    30541 tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat acgcagaact gaactacggg
    30601 cacgagggtt ctcagccacc tcttcttcgc ccggcatcaa cttacctagt gctcgcaact
    30661 gacgcccccc cagttttttg agctgctctt ctgtcatcgg taaccctgcc ggaacttgcg
    30721 gaccgcggct gttttcacgc ataaaacgtt tcacaatgcg gtcttcaagt gagtggaagc
    30781 tgatgatcga aagccgcccc cccggggcca gcacattgag cgagcttttt agcgcctgct
    30841 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    30901 gatgtttaaa cttatctttc accggcgttg cggcagccac gacttccgcc agttctttgg
    30961 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgagcaatg cgtttggcaa
    31021 aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    31081 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    31141 gcataaagga aaagccgcgt tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    31201 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacatattcg cccagcgcag
    31261 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    31321 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    31381 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    31441 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    31501 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    31561 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    31621 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    31681 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    31741 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    31801 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    31861 tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga taagcgccct
    31921 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    31981 actttgcccc acaatttccc actaaaagga gtttacggag cggaggaaaa gcttgtcaag
    32041 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    32101 tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    32161 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    32221 tttgcgacgc gagcggcatt ttaggacata tcttctctgg ttaacagtcc ctgttgcgtc
    32281 tgtgtggcgc ttgatgccag caggtttgcg caaaaacaaa gaaaagtgtt tgttaaatcg
    32341 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcaata tcatttaatt
    32401 attgtgcatt tcagcaggtg agttaattaa cgggcgggtt taaaagcatt tatcaataat
    32461 agccaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctcaacgcgc
    32521 cagagagaaa ttcacctggc gcgtactttt gttcgcggtt tagctacggc tgagcacgcc
    32581 gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac gcggttcgtc
    32641 caggctcgcc aggacaatct ccagcacacg ttctgcgaca tcgcggtgac gttgagccac
    32701 tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg tggcaattgc
    32761 caggtcagaa ggcagtttgc cgtcgcgacg tagcgtgaca tccatcaccc cttgcaacaa
    32821 cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc atttttcgaa
    32881 taactgggca gctgcctccc gctcatagct gttggcataa aggaaatgca cttcgcgcgg
    32941 atcatctttc caggcagtac ggaagccttg ttcacgcagg aagctgacag aaagctccgg
    33001 cagcgcgcca agataaagca ccgtctcggc gggaaactta cgtaactctt ccgccagcat
    33061 ttcggcatca tcctgatcgg caccaaccac gctggtgaag tgttcacgat cgagggcgcg
    33121 gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag gatgctcagg
    33181 tggcaacgac gtcgaaacaa taatggcatc aacctgacgc tgtaaaaggt gctcaataca
    33241 gcgcatttcg ttgtcgggct gatcttccga gcaggcaatc agcagttgat aaccccgttg
    33301 ccgcgcctgg cgttcaagat agttagcgat gcgggtatag ctggtgttct ccagatcggg
    33361 gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg
    33421 gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt cgctcacacg
    33481 gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca ctcccgccag
    33541 ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa accatagcca
    33601 ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac tgcggcaact
    33661 gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg
    33721 caaattgcgc attgagatca ttctcaacgc attattttat cgccgcgcga aagtccgacc
    33781 acaccagagc gagcaacttc cacaattttc gccacatcgc gaatcgatgc taaaaatgca
    33841 tcaagcttat cgctggtgcc tgctaattga acggtataaa gcgagggtgt gacatcgata
    33901 atttgcccac ggaatatttc cgtattacgt ttcacttcgt cacgcccgta accgctggcc
    33961 tgaattttca ccagcatgat ttcccgctca acgtgcgcgc cctgccccaa ctcgctcacg
    34021 cgcaagacat cgaccagctt gtgtaattgc ttttcgattt gctcaagcac tttttcatcg
    34081 cccacggtct ggatggtcat acgcgataat gtcggatcgt cggttggcgc aacggtcagg
    34141 ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat
    34201 tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc gttttgctta
    34261 accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat
    34321 cgacggtaac atcaacaaac accaggcgat tattgcgcac ctgttccagc gcctcgctaa
    34381 gtttgctttc cagttcttgc ggatgagaga tctggatccc gacgtgacca taggcttccg
    34441 ccagacggac gaagtctggt aaggactgca tataagattg tgaatgacgg ccggaataga
    34501 tcatgtcctg ccactgcttc accatcccca gatagcgatt attgagattc accaccagta
    34561 cgggcaactc gtattgcaag gcggtagaca gttcctggat gttcatctga atactgccgt
    34621 caccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc agtgccgcag
    34681 gtaaaccaaa acccatcgtg ccgaggccgc cagagttgat ccagcgacgc ggtttgtcga
    34741 atggataata aagcgcagca aacatctggt gctgcccgac atcggacgtc acgtaagcgt
    34801 ccccattagt caaccgccaa agagtctcga tcaccgcctg cggtttaatc ttttcactgt
    34861 gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatctgctgc caccagtcgc
    34921 ggatctcatc cagcggttga tgggcggatt cttgcgacag gagttcaagc atttgttcta
    34981 ggacctggcg agcatccccc acaatcggga tatcagcagt cacggtttta gaaattgagg
    35041 ttggatcaat atcgatatgc agaacagtgg catttgggca gtattttgcc agattgttcg
    35101 tcgttcggtc atcaaaacgt gccccgacgg cgaaaatcac atccgcgtta tgcatcgtca
    35161 tattggcttc gtaggtaccg tgcattccca acatgcccag tgcctgacga tgcgttgccg
    35221 gaaacgcccc cagccccatc aaagaggaaa caacgggcag attcaacgcc tccaccgctt
    35281 ctttcaactg cagatggcag cccgccgtga ttgccccacc gcctacgtag acaaccggtt
    35341 tttttgccgc caccagcgtt tgcagagcgc gcttaatttg ccctttatgc ccggaggtcg
    35401 tgggattgta agaacgcata ctgaccgact ccggccagac atagggtaat ttgttcgccg
    35461 gatttagaat atctttcggt aaatcaacga ccacgggtcc aggacgacca cttgccgcta
    35521 gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt
    35581 gtttaaccac cgggcgagaa atccccacca tgtcgcactc ctgaaaggca tcataaccta
    35641 tcaacgaagt cgctacctgc ccggaaagga caactaatgg aatggaatcc atataagcgg
    35701 tggcaatgcc agtaatcgca ttggtcgccc ctggacccga cgttaccagc acgacgccga
    35761 tttccccggt cgcgcgcgcc agaccatcgg ccatatgcac cgccgcctgc tcatggcgaa
    35821 ctaaaacatg atcgatacca cccacggtat gcaatgcatc gtaaatatca aggaccgcgc
    35881 ctccaggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg acgaccatct
    35941 cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac
    36001 acattaaccg tccgatcttg cttaagcaat tggcagacag agacataaac acggggtaat
    36061 aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac agaggatgaa
    36121 attgataata aatcggctga atcctacaac ttacagcaat ttctctattt ttttaattaa
    36181 tcctaaaata gagagcacaa atattcagaa taaaattcta cattcaataa aaaatgcaga
    36241 ataaaccaga cattgatgtc tgacctattc tgcattcagt tagcgtttgc aaattgagac
    36301 taattgctct tccatccact gatggccttt atcgcgcccg gcagcttcat gccaggagag
    36361 ataacaggtt ctgctgtttt gttttaacgg cagcggtaat acctgtaact ctaaggattc
    36421 agcgaactct tcagccaacc aacgcggcgc aatagcaacc agatgcgttt gcgacaccac
    36481 gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct tatctaccgt
    36541 gtcataccag gtttgactaa atgacgcgaa acgatcgagc gaaaccgccg catgttgttc
    36601 gttataaaca tcatgtttca gtaacgggcc tttaattgtt ggatgatttt tgctggctac
    36661 caacaccatt tcatctttaa ataatggcac gctggtaaat tcaggacgat ggaagtcttc
    36721 ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt tctgatttaa
    36781 tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat tataaatctg
    36841 cgaggtcaga atgctgtcta acgggctgca gacacaaaga tgaaatacac gttcactact
    36901 ggcaggctca aagcctgaac ctggtaattc attttgtact aattgcaatg cctgacgaac
    36961 tgagccaaaa agttgaaatg cgcgagcagt cggttgaata ccacgaccat aacgaacaaa
    37021 aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg caggttgcga
    37081 cattcccaga gcatgagcgg cacgggtgat gttttgctcc tgcatcacgg catcgaaaac
    37141 ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg ccgtctctgg
    37201 atgatctgtt tgtacctctg gcatacttaa ctccactgtc acacttaact ccctttccct
    37261 tattgg
//
LOCUS       DACADA010000029        36772 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.029, whole genome shotgun sequence.
ACCESSION   DACADA010000029 DACADA010000000
VERSION     DACADA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.029"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(314..2635)
                     /locus_tag="ISB17_001459"
     CDS             complement(314..2635)
                     /locus_tag="ISB17_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP1979404.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMEHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            complement(2732..3244)
                     /gene="greB"
                     /locus_tag="ISB17_001460"
     CDS             complement(2732..3244)
                     /gene="greB"
                     /locus_tag="ISB17_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAP1979405.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            3436..4155
                     /gene="ompR"
                     /locus_tag="ISB17_001461"
     CDS             3436..4155
                     /gene="ompR"
                     /locus_tag="ISB17_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAP1979406.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4152..5504
                     /gene="envZ"
                     /locus_tag="ISB17_001462"
     CDS             4152..5504
                     /gene="envZ"
                     /locus_tag="ISB17_001462"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAP1979407.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5581..7203)
                     /gene="pckA"
                     /locus_tag="ISB17_001463"
     CDS             complement(5581..7203)
                     /gene="pckA"
                     /locus_tag="ISB17_001463"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP1979408.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7581..9305
                     /locus_tag="ISB17_001464"
     CDS             7581..9305
                     /locus_tag="ISB17_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAP1979409.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGLLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9403..9600)
                     /locus_tag="ISB17_001465"
                     /pseudo
     CDS             complement(9403..9600)
                     /locus_tag="ISB17_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9608..10816
                     /gene="tnpB"
                     /locus_tag="ISB17_001466"
     CDS             9608..10816
                     /gene="tnpB"
                     /locus_tag="ISB17_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP1979410.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(10939..11817)
                     /gene="hslO"
                     /locus_tag="ISB17_001467"
     CDS             complement(10939..11817)
                     /gene="hslO"
                     /locus_tag="ISB17_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP1979411.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(11842..12243)
                     /gene="hslR"
                     /locus_tag="ISB17_001468"
     CDS             complement(11842..12243)
                     /gene="hslR"
                     /locus_tag="ISB17_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAP1979412.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(12254..12922)
                     /gene="yrfG"
                     /locus_tag="ISB17_001469"
     CDS             complement(12254..12922)
                     /gene="yrfG"
                     /locus_tag="ISB17_001469"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAP1979413.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(12987..15122)
                     /gene="igaA"
                     /locus_tag="ISB17_001470"
     CDS             complement(12987..15122)
                     /gene="igaA"
                     /locus_tag="ISB17_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAP1979414.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMLFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            15442..16002
                     /gene="nudE"
                     /locus_tag="ISB17_001471"
     CDS             15442..16002
                     /gene="nudE"
                     /locus_tag="ISB17_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAP1979415.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(16168..18720)
                     /gene="mrcA"
                     /locus_tag="ISB17_001472"
     CDS             complement(16168..18720)
                     /gene="mrcA"
                     /locus_tag="ISB17_001472"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAP1979416.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            18840..19619
                     /gene="hofM"
                     /locus_tag="ISB17_001473"
     CDS             18840..19619
                     /gene="hofM"
                     /locus_tag="ISB17_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAP1979417.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            19619..20158
                     /locus_tag="ISB17_001474"
     CDS             19619..20158
                     /locus_tag="ISB17_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HAP1979418.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            20142..20582
                     /gene="hofO"
                     /locus_tag="ISB17_001475"
     CDS             20142..20582
                     /gene="hofO"
                     /locus_tag="ISB17_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAP1979419.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            20572..20976
                     /gene="hofP"
                     /locus_tag="ISB17_001476"
     CDS             20572..20976
                     /gene="hofP"
                     /locus_tag="ISB17_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAP1979420.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            20888..22126
                     /gene="hofQ"
                     /locus_tag="ISB17_001477"
     CDS             20888..22126
                     /gene="hofQ"
                     /locus_tag="ISB17_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAP1979421.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            22527..23048
                     /gene="aroK"
                     /locus_tag="ISB17_001478"
     CDS             22527..23048
                     /gene="aroK"
                     /locus_tag="ISB17_001478"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAP1979422.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            23105..24193
                     /gene="aroB"
                     /locus_tag="ISB17_001479"
     CDS             23105..24193
                     /gene="aroB"
                     /locus_tag="ISB17_001479"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP1979423.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHSIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            24285..25571
                     /gene="damX"
                     /locus_tag="ISB17_001480"
     CDS             24285..25571
                     /gene="damX"
                     /locus_tag="ISB17_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAP1979424.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            25678..26514
                     /gene="dam"
                     /locus_tag="ISB17_001481"
     CDS             25678..26514
                     /gene="dam"
                     /locus_tag="ISB17_001481"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP1979425.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            26532..27209
                     /gene="rpe"
                     /locus_tag="ISB17_001482"
     CDS             26532..27209
                     /gene="rpe"
                     /locus_tag="ISB17_001482"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP1979426.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            27202..27960
                     /gene="gph"
                     /locus_tag="ISB17_001483"
     CDS             27202..27960
                     /gene="gph"
                     /locus_tag="ISB17_001483"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAP1979427.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            27953..28957
                     /gene="trpS"
                     /locus_tag="ISB17_001484"
     CDS             27953..28957
                     /gene="trpS"
                     /locus_tag="ISB17_001484"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP1979428.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            29119..30024
                     /locus_tag="ISB17_001485"
     CDS             29119..30024
                     /locus_tag="ISB17_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979429.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            30041..30403
                     /locus_tag="ISB17_001486"
     CDS             30041..30403
                     /locus_tag="ISB17_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP1979430.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            30487..31650
                     /locus_tag="ISB17_001487"
     CDS             30487..31650
                     /locus_tag="ISB17_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAP1979431.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            31651..32877
                     /locus_tag="ISB17_001488"
     CDS             31651..32877
                     /locus_tag="ISB17_001488"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP1979432.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            32874..33752
                     /locus_tag="ISB17_001489"
     CDS             32874..33752
                     /locus_tag="ISB17_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAP1979433.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            33763..34116
                     /locus_tag="ISB17_001490"
     CDS             33763..34116
                     /locus_tag="ISB17_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAP1979434.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            34128..35432
                     /locus_tag="ISB17_001491"
     CDS             34128..35432
                     /locus_tag="ISB17_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979435.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            35444..36529
                     /locus_tag="ISB17_001492"
     CDS             35444..36529
                     /locus_tag="ISB17_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP1979436.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(36637..>36772)
                     /locus_tag="ISB17_001493"
     CDS             complement(36637..>36772)
                     /locus_tag="ISB17_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAP1979437.1"
                     /translation="AVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ
                     "
BASE COUNT         8565 a         9204 c        10092 g         8911 t
ORIGIN      
        1 tggtttctat cagcaatact taaaatgtgt gtgaatgttg gtttgaataa taatctgact
       61 caatgttgat atggctcaag cttatatcgc agcgaaaata taattcgcat gacgaattat
      121 tagggctggt taatgtttaa ttaaggtttc agttgttaat gaagtgataa atattaacgg
      181 gatatattat gcgtttgctt gtggaataaa aaggccggag atattctccg gcacttttat
      241 gtcaggcaag gcgttcgttg ccagatgtgg cgtgaacgcc taatcgcgca ggtttagggg
      301 ccagtccggg cgtttaacgt tttttgccca ttgccgccgc cagcgcatcc atcatcgcgc
      361 tgttaccggc aggctgcact tcacgcccac gcggtttggc tgccgggcgg ttgttttgcg
      421 ggcgttcatt accgccgccg cgacgggcgt tggtttcgcc aggctgctcg tccagacgca
      481 tggtcagggc gatacgttta cgctgaagat ccacttccag cactttcacc ttcacaatgt
      541 cgcccgcttt caccacggta tgcggatctt ccacaaactt gttcgacaat gaagagatgt
      601 gaaccaggcc gtcctgatgc acgccaatat cgacaaacgc gccaaagttg gtgacgttgg
      661 tcaccgcacc ttcgaggatc atacccggtt gcaggtcgtt cattgtctcg acaccatcgg
      721 caaactgagc ggttttaaat tccggacgcg gatcgcgacc cggtttttcc agctctttga
      781 tgatgtcggt taccgtcggc acaccgaatt tctcatcagt aaagtcagac gctttcaggt
      841 tacgcagttc gctgctgtta cccatcagat ctttcagtgc ctgctgtgtt gctgccagaa
      901 tgcgttccac caccggatag gcttccgggt gaacggtaga agcgtccagt gggttatcac
      961 cgtggttaat gcgcaagaag cccgcgcact gctcgaaggc tttcggcccc agacggctca
     1021 cttttaacag ttgctgacgg ttctggaact gaccgttctc atcgcgccag gctacgatgt
     1081 tttgcgccat catgcgcgtc aggcccgcca cgcgggttaa cagaggaaca gaagcggtgt
     1141 tgagatcgac gccaacggcg tttacgcagt cttctactac tgcatccagt ttgcgtgcca
     1201 gctgcgtctg gctgacgtca tgctgatact gacctacgcc gatagatttc ggatcgattt
     1261 tcaccagctc cgccagcgga tcctgcaaac gacgggcgat agacaccgcg ccacgcagcg
     1321 aaacgtcgag atccgggaat tcctgtgctg ccagctcgga agccgagtaa accgacgcgc
     1381 cagcttcgct gacgatcact ttctgcgcgg tcactttcgg gaactgcttc tgcacgtcga
     1441 gatagaaacg ttcggtttcg cgggaagctg taccgttgcc gatcgccacc agttcaacgt
     1501 tatgcttttc acacagcgca gcaacggtca tcgctgcttt tgcggcctgc ccggtatgcg
     1561 gataaatggt atcggtcgcc accagtttac cagttgcatc gactaccgcc acttttaccc
     1621 cggtacgcag acccggatcg aggcccatcg ttgcacgcag tccggcaggg gccgccatca
     1681 gcagatcgtg caggttacgg gcaaagacgt tgattgcttc atcttccgcg cgttcgcgca
     1741 cggtgcccat cagttcagtt tccagatgca tcaacacctt gatgcgccac gtccagctca
     1801 ccacaccttt gcgccagcta tccgccgggg cattgttcag gcgcaggcca aggtgttcca
     1861 tgatgatttg ctcgcaatag ctctctttcg gcggctcttc gaactgcgga tcggcattca
     1921 gcgaaagctg aagtatgcct tcgttacgac cacggaacat cgccagtgcg cggtgagaag
     1981 gcaccgttga cagtggttcg tgatgatcga aatagtcgcg gaattttgcc ccttcctctt
     2041 ctttaccgct caccacggtt gagaccagat gcgcgttctt ccacagataa tcacgcactt
     2101 tcgccagcag cgcggcatct tcggcaaacc gttccatcag gatatagcgc gcgccgtcca
     2161 gcgcggcttt ggtatctgcc acgcctttat cggcatcaat atattgtgca gcggcgactt
     2221 ctggcgtgtg tgacggatcg ctccacagca gatcggccaa cggctcaagc cctgcttcaa
     2281 tggcgatttg cccacgggtg cggcgtttag gtttgtaggg caggtagagg tcttcgagtt
     2341 cggttttgct tagggtggcg ttgatggcat tcgccagatc atcggtgagt ttgccttgct
     2401 cggaaataga cttgaggatc gcctgacgtc tctcttccag ctcgcgcaga tagctcagac
     2461 gcgtttccag attacgcagc tgcgtgtcat ccagaccgcc ggtgatttcc ttacgataac
     2521 gtgcgataaa cggcacggta ttcccttcgt caagcaggcg aacggcagcg tcaacctgtt
     2581 ccgggcgcgc ctgaatttca cccgcaataa tgcggcagaa cgaatcattc atcatggttt
     2641 gagttcatct tttcggatca aaaatcaggg gatagttata cggactggct ggcaaaaatg
     2701 ccagccatcg gcaggaggtt aagactcttc cttacggttt cacgtactcg atagcattaa
     2761 cataccagct cgcttccccg gcaggggtat tcaccaccgc cagatcgccg acttcttttt
     2821 tcagcaatgc gcgggccatc ggggaatcga tagagatgta atctttacgg ccaaaaattt
     2881 catcgtagcc gacaatacgg aaacggtgag tcacaccatc gtcgttttca atctccaccc
     2941 acgcaccaaa aaagactttg ccttcctgct gcggggagta atcgacgatt ttgagatttt
     3001 ccatgcattt agtgagatag cgcacgcgac ggtcgatttc acgcaggcgc tttttattat
     3061 actgatagtc agcattttcg ctgcggtcgc ccagacttgc ggcccaggtc actttctttg
     3121 tgacctccgg gcgttcttca cgccagagat aattaagctc ttgtttgagt ttttcatacc
     3181 cttcccgggt aaccaggggc gttttcatct cgttgattcc ctttgtctgt ttgataatgc
     3241 gcacattggg tataacgtga tcatatcaac agaatcaata atgtttcgcc gaataaattg
     3301 tatacttaag ctgctgttta atatgctttg taacaattta ggctgaaatt cataccagat
     3361 ttagctggtg acgaacgtga gcttttttaa gaatacacgc ttacaaattg ttgcgaacct
     3421 ttgggagtac aaacaatgca agagaactac aagattctgg tggttgatga cgacatgcgc
     3481 ctgcgcgcgc tgctggaacg ttatctcacc gaacaaggct tccaggttcg aagcgtcgct
     3541 aatgcagaac agatggatcg cctgctgact cgtgaatctt tccatcttat ggtactggat
     3601 ttgatgttac ctggtgaaga tggcttgtcg atttgccgac gtcttcgtag ccagagtaac
     3661 ccgatgccga tcattatggt gacggcgaaa ggggaagaag tggaccgtat cgttggcctg
     3721 gagattggcg ctgacgatta cattccaaaa ccgtttaacc cgcgtgaact gctggcccgc
     3781 attcgtgcgg tgctgcgtcg tcaggcgaac gaactgccag gcgcaccgtc acaggaagag
     3841 gcggtaattg ctttcggtaa gttcaaactt aacctcggta cgcgcgaaat gttccgcgaa
     3901 gacgagccga tgccgctcac cagtggtgag tttgcggtac tgaaggcact ggtcagccat
     3961 ccgcgtgagc cgctctcccg cgataagctg atgaaccttg cccgtggtcg tgaatattcc
     4021 gcaatggaac gctccatcga cgtgcagatt tcgcgtctgc gccgcatggt ggaagaagat
     4081 ccggcgcatc cgcgttacat tcagaccgtc tggggtctgg gctacgtctt tgtaccggac
     4141 ggttctaaag catgaggcga ttgcgcttct cgccacgaag ttcatttgcc cgtacgttat
     4201 tgctcatcgt caccttgctg tttgtcagcc tggtgacgac ttatctggtg gtgctgaact
     4261 tcgcgatttt gccgagcctc cagcagttta ataaagtcct cgcgtacgaa gtgcgtatgt
     4321 tgatgaccga caaactgcaa ctggaggacg gcacgcagtt ggttgtgcct cccgctttcc
     4381 gtcgggagat ctaccgtgag ctggggatct ctctctattc caacgaagcc gccgaagagg
     4441 ccgggctgcg ttgggcgcaa cactatgaat tcttaagcca tcagatggcg cagcaactgg
     4501 gcggtccgac ggaagttcgc gtcgaggtta acaaaagttc gcctgtcgtc tggctgaaaa
     4561 cctggttgtc tcccaatatc tgggtacgcg tgccgctaac cgaaattcat cagggcgatt
     4621 tctccccgct gttccgttat acgttggcga ttatgctatt ggcgataggc ggggcgtggc
     4681 tgtttattcg tatccagaac cgaccgttgg tcgatctcga acacgcagcc ttgcaggtgg
     4741 gtaaagggat tattccgccg ccgctgcgtg aatatggcgc ttcggaggtg cgttccgtta
     4801 cccgcgcctt taatcatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc
     4861 tgatggcggg ggtaagccat gatctgcgca cgccgctgac gcgtattcgc ctggcaaccg
     4921 agatgatgag cgagcaggat ggctacctgg cagaatcgat caataaagat atcgaagagt
     4981 gcaacgccat cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg
     5041 aaatggcgga tctcaacgca gtactcggtg aggttattgc tgccgaaagt ggctatgagc
     5101 gggaaattga aactgcgctt taccccggca gcattgaggt gaaaatgcac ccgctgtcga
     5161 tcaaacgcgc ggtagcgaat atggtggtca acgccgcccg ttacggcaat ggctggatca
     5221 aagtcagcag cggcacggag ccgaatcgcg cctggttcca ggtagaagat gacggtccgg
     5281 gtattgcgcc ggaacaacgt aagcacctgt tccagccgtt tgttcgtggc gacagtgcgc
     5341 gcactattag cggcacggga ttagggctgg cgattgtgca gcgtatcgtg gataaccata
     5401 acgggatgct ggagcttggc accagcgaac ggggcgggct ttccattcgc gcctggctgc
     5461 cagtgccggt aacgcgggcg cagggcacga caaaagaagg gtaaataaaa cgggaggcga
     5521 aggtgcctcc cgttttgctt tctataagat actggataga tattctccag cttcaaatca
     5581 ttacagcttc ggaccagccg ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc
     5641 gaagttgtcg ataaacagtt tcgccagcgt ttcggctttt tcctgccact gttccggaga
     5701 agcgtaggtg ttacgcggat cgaggatctt cgtgtctacg cccggcagtt cggttgggat
     5761 cgccaggtta aacatcggca gagtgaaggt ttctgcgtta tccagcgaac cgttgaggat
     5821 ggcgtcgata atagcgcggg tatctttaat ggagatacgt ttgccagtgc cgttccagcc
     5881 ggtgttaacc agatacgcct gcgcacccgc tgcctgcata cgtttcacca gcacttctgc
     5941 gtattgggtc gggtgcagcg acaggaacgc cgcgccgaag caagcggaga aggttggcgt
     6001 cggttcggtg atgccgcgct ctgtaccagc cagtttggcg gtgaagccag agaggaagtg
     6061 gtactgggtt tgatcggcag tcagacgaga aaccggcggc agcacaccaa aggcgtctgc
     6121 tgtcaggaaa ataactttag ttgcgtggcc cgctttggaa accggcttaa cgatgttatc
     6181 gatgtgatag atcggataag aaacgcgggt gttctcggtt tttgaaccat catcaaagtc
     6241 gattgtgcca tcttcacgca cggtgacgtt ttccagcagc gcatcgcgac ggatagcgtt
     6301 gtagatttca ggttccgctt ctttcgacag cttgatagtt ttcgcgtagc agccgccttc
     6361 gaagttaaac acaccgtcat catcccagcc gtgctcgtca tcgccaatca ggcggcgttt
     6421 cgggtcggtg gaaagggtgg ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc
     6481 gcctttctcg ccaacgttgg cggagcagtg catagaagcg atacctttca gcggcagcag
     6541 gtagttcatc atcgagaaca tccctttctt catttcgccg ccgtaccagg tgccgccaat
     6601 cagctgcatg cgctcggtca ggttaaacgc cacgaagttt tcggagttca gaccctgttc
     6661 tttccactgc gggttagtgc acttcgcgcc gttcataacg ataaagtctg gtttgaaacc
     6721 tgccagctct tcatcgctcg ggcgaataaa catgtttttg acaaaatgcg cctgccaggc
     6781 cacttcggtg atgaaacgga cggaaagacg agtatccggg ttcgcaccac agaaggcgtc
     6841 gacaacgaac agacgtttgc cggaaagctg cctggtcacc aggcctttca gatgctgcca
     6901 ggtttccgga gagagaggtt tgttgtcgtt cttacctttg cctttgtctg cccaccagaa
     6961 agtatcgcga gtggtatcgt cacgaacgat atacttatct ttcggtgaac gaccggtgaa
     7021 gatcccggta tcgacggcaa cggcacccag attagttaac accccgcgct cataaccggt
     7081 caggctcggg tccagctctt cctgatacag cagatcgtag cttgggttgt aaacgatatc
     7141 atgtacgtca ctgataccat aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg
     7201 catttcactg ctccttagcc aatatgtatt gcctgaatag taaagtcttt ttgggggtgt
     7261 taaccgcgac aaggctcata gatttacgta tctggagaaa ttcatatttg acaaaaaact
     7321 ccgtgattcc tgtcacgaaa cggttgctat tatcgcagaa atagcatttt aggtgtggaa
     7381 aatgttctca aaaggttaaa tcttggttta tatggcggga atattgtgag tggaaccgca
     7441 ttcatgaatg aaattgatgc aaattcaatc ggtaaagtat cgattcacca cgctaacttt
     7501 ttttgattat tttctgaaaa aagcaaaata ctactgcccg atgcgataat taacccacct
     7561 tttgattctg gataaccaac atggataacg ttgaactttc accggcaaca cgctggggga
     7621 tgattgctac cggattactt caggggctgg tttgctacct gctgatcgcc tggcttgccg
     7681 gaaaaaatct cagttggatt gtttatggcg tgcctgcgac cgtcgcgttt tcatccgttt
     7741 tactcttctc cgtgatctcc tttaaacaga atcgcctttg gggatggctg gcgctggtgt
     7801 ttattgccac gctgggaatg agcggcttgc tgaagtggca aactgatggc atgaccccct
     7861 ggagagctga aaaggcgctt tgggattttg gctgctatct gctgctgatg gcaatgttgt
     7921 tgctaccgtg gatacaacaa agccttcgca tccgcaatga cagtagccgc tatcgctact
     7981 tttaccagtc agtatggcat aacgtactta tattactggt gatttttctc gccaatggcc
     8041 taacgtggct ggtactttta ctgtggagtg agttgtttaa acttgttggc atcacgtttt
     8101 tcaaaacgct cttttttgca accgactggt ttatttatct cacgttaggt ctggttaccg
     8161 cgctggcggt gatcctcgcg cgaacacagt cacgtttaat cgactctatt caaaagttgt
     8221 tcacgttaat cgccacgggg ttgctgccgt tagtatcatt gctaaccctg atgtttatta
     8281 tcaccctgcc gtttacgggc ctgagcgcga tttctcgcca catctccgcc gccgggttgc
     8341 tgttgacgct ggcctttttg caattgatct taatggctat tgtccgcgat ccgcaaaaag
     8401 cgtcacttcc ctggacgggg ccattgcgtt gcctgattaa aaccgctttg ctggttgctc
     8461 cgctgtatgt gttcgtcgcc gcctgggcgt tatggctgcg ggtcgctcag tacggctgga
     8521 ctgtcgaccg cttgcagggc gcgctggcgg tgctggtgtt actggtatgg tcgctggggt
     8581 attttgtcag tatcgtctgg cgtaaagggc aaaatcccct tgatcttcag ggcaaagtga
     8641 accttgcggt ttcgttattg gtgttggtaa tactggtgct tcttaattcg ccggtgctgg
     8701 acagtatgcg cattagcgtg aacagtcata tggcgcgtta tcagagcggt aaaaacacgc
     8761 cagaccaggt aactatctac atgctcgaac agagcggtcg ctatggacgt gcggcgcttg
     8821 agtcgctgaa aagcgatgcc gagtatatga aagacccgaa acgcgcgcgg gatctgctga
     8881 tggcgttaga tggagagcaa catcttcagg aacaggtatc ggaaaaagta ttagccgata
     8941 atgtgttaat tgccccaggt tctggtaaac cggatgcgac attctggtcg gccttaatcc
     9001 aggatcgcta taacgtgatg acctgtattg aaaaagacgc ctgcgtactg gtcgagcaag
     9061 atctgaatag tgatggtcag gcggagcgga tcctgtttgc ttttaatgat gacagagtca
     9121 ttgtctatgg ctttgactca gccagaaaag aatgggacgc gcttgatatg agtttacttc
     9181 cgcgcaaaat aacgaaagaa aaattactca cagctgcgaa ggatggaaaa ctggggacga
     9241 ggccgaaagc gtggcgcgat ctgacggtag atggtgaaac gctggaaata aacttaagta
     9301 agtgacaacg cttttaacgg atacgacgct gtatctgacc aaaataactt gcgatttatc
     9361 cggtgcgcgg taaaaccccg tccttcaggg cggggatata agttcgccat ccatttcaac
     9421 ccgttcagct tcaaaatcag cacatacatt tgaaaagtaa gtgcgtagtt tttctgtcgc
     9481 gtcatggtca aaaatctggc gttggtattt ggcgacaaag accaggtgaa catgcatcag
     9541 gaaaacacaa tgccttccac gccggatatc ggtttctttt ttcatagacc aaaatataat
     9601 ttgacctgtg aaacgactac aggcatttaa attccagtta agacccggtg gtcaacagga
     9661 gcgtgaaatg aggcgcttcg caggcgcatg tcgtttcgtt ttcaatcgtg ctctggcgct
     9721 tcagaatgag aatcatgagg ccggtaataa atacatccct tacgggaaaa tggcttcctg
     9781 gctggttgag tggaaaaatg ccactgaaac gcaatggctt aaagattctc cctcacagcc
     9841 attgcaacag tcactgaaag accttgagcg ggcttacaaa aacttcttcc agaagcgggc
     9901 tgcttttccc cgattcaaaa agcggggaca gaatgatgca ttccgctacc cgcagggcgt
     9961 taagctcgat caggaaaaca gccgtatttt tctgccgaaa cttggttgga tgcgctaccg
    10021 gaatagccgt caggtcacgg gagttgtgaa aaatgtcact gtcagccagt cctgcggtaa
    10081 gtggtacatc agtattcaga cagaaagtga agtatcaact ccggttcacc cttcagcatc
    10141 aatggtcggg ctggatgctg gcgtggctaa actcgccacg ctgtcagatg gcacagtctt
    10201 tgagcctgta aacagttttc agaaaaacca gaagacgctg gcgagacttc agcgacagtt
    10261 aagccgcaag gtcaaattca gcaacaactg gcagaagcag aaacgcaaaa tacagcggct
    10321 gcattcctgt atcgcaaata tccgcaggga ctaccttcac aaagtcacaa cgaccgtcag
    10381 caaaaaccac gcaatgatag tcattgagga tttgaaggtc agcaacatgt caaagtcagc
    10441 agcgggtacg gtcagccagc cggggcgcaa tgtccgggca aaatcaggtt taaaccgttc
    10501 gatactggat cagggctggt atgaaatgcg ccgccagctt gagcacaagc agctctggag
    10561 tggcggtcag gtgcttgctg ttccgccagc gtacacaagc cagcgttgcg cgtgctgtgg
    10621 tcatacagcg aaagaaaacc gcctgtcaca aagtaaattc agatgccagg tatgtggata
    10681 tacagcgaac gccgatgtaa atggcgctcg taacatttta gcggcggggc acgccgttct
    10741 tgcctgtgga gagatggtgc agtcaggccg cccgttgaag caggaaccca ccgaaatgat
    10801 tcaggcgaca gcctgaacgt agcagggatc cacgtccttc agggcgtgga ggatgtcaat
    10861 tcataggcct ggtaagcgca gcgcatcagg ccattacgtc gtaaaatccg gcagagtcct
    10921 ctctgccgga catactcatt aatgaacttg cggatctgcc ggagacgcgt tgttgcggat
    10981 ctcggcaata tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc
    11041 aatttcgcca tcttccgcca ggatgctatc gacttcttca tcaggcagcg ttttcagcgc
    11101 atcggcgcaa cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac
    11161 cgtcacctct tcttcgtgat acaaacgcca caacacttcg tttgccggta aagtcagcag
    11221 ttcttcggtt ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgctgggc
    11281 attttgcgca ggcattacct gcaacaacat accgcctgca gccggtttgc cgtctacgtc
    11341 gccggtgcga ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttccag
    11401 gcaggccgcc agggtatcac cttccagacc aaccacgccc tgatagcgtt cgccttcgct
    11461 cggggtgatg gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc
    11521 tggaatttcg ccctgcacgc gcgccacacc gcgcatctgc tggttgttgt taccgttaat
    11581 aaccgccaga ttcatcggac cgtcgccttg cagctgtacg gtgatatcac catcaaactt
    11641 caacgtagcg gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg
    11701 ctgcggataa tcgtgattct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc
    11761 gccgcgaacg gcaaagtttt caaacagata gcgatgtaat tggtcatgtt gcggcataat
    11821 catctctctt gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg
    11881 gcgctctttt ttgtccgggc gtcggtccgg atgcggcatg gttaaggcat taagtttacg
    11941 tgccagcgcc attttttcgc gtttctctac actttccgca gtctcttcat acagcaagac
    12001 tgcctcgctg gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc
    12061 atttccctgg cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg
    12121 ctgcccgttg taatgcacct taccaccttc gatcatttcg cgggccagcg cgcgggtttt
    12181 ataaaaacgg gcggcccata gccatttatc cagtcgaacc tcaacagcag gtttttcttt
    12241 catggcgtct ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat
    12301 ggcgctgata ctgtttctcg gcaatcccgg agtcaggatt agtcacaccg aggcagtaac
    12361 gaataccaaa ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc
    12421 tttcagcttt aagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg
    12481 gataaccaaa tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga ccggtatgct
    12541 caagttttac cgccaggttg tgcggatgcg cattggtgag caaaattcgc tgtttaccgc
    12601 tggctttcag tgcctcaaga aacggaatgg tatcttcacg cagtacggca cgcggtccca
    12661 tctcggtggt catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt
    12721 accagtttag cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct
    12781 gtggcgtaac tccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt
    12841 tatcgaaggc gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct
    12901 gccaggcaat gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg
    12961 gtagcataac ctgccgcgca aacgtgttat tcgataaggc tttcggaagg ggtgatcagt
    13021 tgcgggttca ggcagctttc ataataggcc tgaatcttca tcatgcgagt acgatgacgc
    13081 tggtaacgac gccaggcctg tacgccatta taaatggcgc tacccagcat cgtcagtagc
    13141 agcagcgtgg tgctgagata gcgccacaga ccggaacgat ccgggatcgg atgaaggcca
    13201 atatgctgcg taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac
    13261 ggcgtatgca tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcgga
    13321 tagtcgtaaa gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg
    13381 acaatcagga acccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca
    13441 tgcgtgataa acggcgcggt agaggtggcc accaggttat ccagcgattc cgcactcacc
    13501 gggcgtaata acacattcac gccatcgagt ttcccggcgt tggcgcgttt taccagcgcg
    13561 tcccagtctt tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct
    13621 ttggcagaac acagatccgc tgtcttcagt actatgtcgc caaaatcatc aagcaatacc
    13681 atgccggatt tttgaattgc tgaacgtaat gaggcgctga cgcgagattc atcttccggt
    13741 ttagggtgca gctggcgatt aactgcttcg gtcaatgccg tcgctttgtt gaccaattca
    13801 gattctggta atggcaatga gcgggcgtcg ttccagatga tctgcgagca gtcaaacggt
    13861 aaaaaaggtg agttggtttt cgcgctccag gtaccggaag tgcgaatatt acacattccc
    13921 gtaccgctaa tacgcaatgt atcgccaacc cgcacgccag cgtcagccag ttgtttcacg
    13981 ctggtggctt caatggtctg cgcgcctttc atccacgaga gggtgaattt cagcggcata
    14041 tccagcggga tccagaataa cagcataaac agcaccagta gcgagcccgc agcgataatc
    14101 gtactgcgca gccagtgctg taacggaaag ttttttactt catcatggag cgaaagatat
    14161 cgcccctgac gcactacgtg gcggtcgaga tagatatcga tatcggtttg ttggccgaga
    14221 tcctgagcaa tgtagggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa
    14281 atattgttga tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg
    14341 cgcagacaat gaatttcccg cagggaggat tttgccgggg gcgcgaatag cccccacaga
    14401 cctgcgccca gcagcagtaa cgcgccgcct gccagccacg gaacaaagac atccggggta
    14461 atcaggcaga aaaagagcat caggaaggag gcgacaatca gcaacgcttc gcgcagcccg
    14521 cgcggacgac tcagcgcata ttcttcatgc gtttctttgc gaatattgag cagctctatt
    14581 tgctcgctct cttccccgcg aatcgacgcc tgcgttgacg gaacaggttg caaggcatag
    14641 ccgcgaattt cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccagcggc
    14701 agcgaatcgg tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaaa
    14761 gggggcaggt ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta
    14821 gagatgccat aacgcgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc
    14881 agcgccagag agatcggtgc cgcgctggct cccgttgggc caggcacctg taatacctgc
    14941 gtcaggctct caagataatt ttcaacggcg ctacgttctt cagatgtgag tttacgcgtt
    15001 tgcgcatccg cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc
    15061 agccatcccg caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc
    15121 atgctttccc catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat
    15181 gattctgcgg ttggctatcg gcaagcgtgg agtttaactt gagcatcatt aagcacatca
    15241 cagtaaccgc tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt
    15301 atttcaacct attgactttt aatttgattt gaggacgatt atttattaag ttacataaat
    15361 gtaaataacg cgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga
    15421 tttcacagca tagatcccac gatgagcaaa tcattacaaa aacccaccat tctgaatgtt
    15481 gaaactgtag cccgttcccg actgtttacc gtcgaaagcg tggatctgga gttcagcaat
    15541 ggcgtgcggc gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg
    15601 ccgattgtgg acgatcacct gatcctgatt cgcgaatacg cagtgggaac tgaatcctac
    15661 gaattaggtt tttcgaaagg attaattgat ccgggtgaaa gcgtctttga agccgctaac
    15721 cgcgagctaa aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc
    15781 agcatggcac cgtcttactt ttccagcaaa atgaatatcg tggtagcgca agatctctac
    15841 ccggaatcac tggaaggcga tgagccagag ccgttaccac aggtgcgctg gccgctggcg
    15901 catatgatgg atttgctgga agcccctgac ttcaacgaag cgcgtaatgt cagcgcgctg
    15961 ttcctcgtgc gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttacctg
    16021 atgcataatg cttatcaggc ttacgaagtt cctgcaattt attgaatttt caattttttg
    16081 ttggccggat aacgcgttca cccgcatccg gcataaacaa gtgcactttg tcagcaaact
    16141 gaaaaaggcg ccgaagcgcc tttttaatca gaacaattcc tgtgcctcgc cattatcgat
    16201 aatggtcgtt cccacctcgt gcaccgcctg ttgtgtcggc tgcgtacctt cgatgaaata
    16261 ctcttcgcgg ctgttgccac cattagctaa ttgcccggtg ctgcgatcga tattcaccgt
    16321 cacaataccc ggtggcggcg tcagcggctg ttccggtaca ccttcaagaa cggctttcat
    16381 ataagcgtcc catgcaggct gggcactctt ggcaccgcct tcgtaacctg agatctgatc
    16441 tttaatcgct ccggaagccg ttgtatgacc gagattacga cggtgatcat caaagccaat
    16501 ccagaccgag gtcacaacgc ccggaccgta acccgagaac cacgcatctt tcgaactgtt
    16561 agtggtaccc gtttttccgc ctatatcatg ccgttgtaaa tcacgacctg ctcgccagcc
    16621 agtaccctgc cagcctggtt caccaaaaat attggtgttc aaggcactct taatcaggaa
    16681 tgccagcgga gtgttgatga cgtgcggtgc gtactcctgc gcgccggtct tcgccactaa
    16741 cgcctgattt gcctgctcca gctgcggcat tggtacagaa acattctgct gctcgcggga
    16801 gatagcgaca tcttcaacat cgttattttc cagcacgttc gatttctgcg tatcaccgta
    16861 aatcaccgga atatcgcatt ccgggcaggc tactttcggt ttcgcttcga aaattacgcc
    16921 gccctgatcg ttttcaattt tgctgataaa ccacgggtcc accaggaagc cgccgttcgc
    16981 catgaccgcg tagccgcgcg ccacctgcat tggggtgaag gaagctgaac ccagcgccag
    17041 cgattcggtg tggacaatgt tttgtgccgg gaagccgaag cgttgcagat attctgcagc
    17101 gtagtcgacg cccatcgccc gcattgcgcg taccatcacc acgtttttcg actgacccag
    17161 cccctgacgt aagcgaattg gaccggcata ttgcggtggt gagttcttcg gctgccagtc
    17221 agaaccggca cctgcatccc agcgggaaat tggcacatcg ttcaacatac ttgccagcgt
    17281 cagaccttta tccatcgccg cggtgtagag gaacggtttg atgttggaac ccacctgacg
    17341 cagtgcctga gtggctcggt taaacttgct ctgattgaaa tcaaagccac cgaccagcgc
    17401 cataacggca ccgttttgcg gattgatcga caccagcgcc gagttcactt ccggcacttg
    17461 cgccagccac catgcatcgt caacttgacg aacccagatt tgctgacccg tttgcagaac
    17521 atcggtcact ttacgcggcg tcggtccctg ctgagtatcc gaacggtaag gacgcgccca
    17581 gcgaacacct tccatactca atacgacggt cgacccgtct gccagcatcg ccgttgcttc
    17641 ctgaggattg gcgctggtga ctgcggcagg cagcagcgga ccataggtcg gcagcgcctt
    17701 cagcgtatcg gtaatcttgt tgttatccca cgccgattcg cccactttcc acagcacatt
    17761 tgccgggccg cgatagccgt ggcgcatgtc gtagtccagc acgttattac gtaccgcctg
    17821 ctgtgcggcc tgctgcactt tgcgggtgat ggtggtgtaa atgcgataac cgtcttcata
    17881 ggcactttcg ccataacggt tatacatctc ctggcgcacc atttcgctca ggtatggcgc
    17941 agagaaagca atctccggcg cgtgatagtt agcgttaatc gcctcagtgc gtgtctgatc
    18001 gaactgttgt tgggtgatat acccttcatc cagcatccgc gacagcacga cgttacgccg
    18061 cgcgacggca cgatccattg agtagagcgg gttgaaggtg gaaggcgctt tcggcagccc
    18121 ggctatcacc gccatttcgt tcaacgtcag ttggtcgaca gttttaccga aatagacttg
    18181 tgccgcagca ccgacaccat aggcgcggta accgaggtaa atcttgttca gataaagctc
    18241 gaggatctcg tctttcgtta gcaactgttc aatgcgaatc gcgaggaaga cttccttaat
    18301 cttgcgcatc agcgtgcgtt ctggactgag gaagaagttt ctcgccagct gctgggtaat
    18361 ggtactcgcc ccttgtgacg cgtgaccgga gaacagcgcc acgcttgccg cacggaagat
    18421 ccccaccggg tcaacgccgt gatgctcgta gaagcggctg tcttccgtcg cgataaaggc
    18481 tttcaccatc tccggcggga tttgatccaa cgtaaccgga atacgacgtt tctcaccgta
    18541 ttgagcaatc agctcgccat cggcgctgta aatctgcatc ggaatttgca ggcgaacatc
    18601 ttttaatgtc gccacatccg gcagttgtgg ctcgatgtag cggtataggc cataaatcga
    18661 gcctgctccc agcagaatgc aacagactgc aaggatcaaa aaatacttta cgaacttcac
    18721 tggaaatttc ccatttagtt tcatttgggc agtttataaa caaacgcgcg gtagtataaa
    18781 ggcaagccag acgcattgat atacccgtca gagtgacggg tgataaggag atcatcacaa
    18841 tggcatttaa gaactggcaa attggtttgc atttacaaca gcaagaagcg gtagcggttg
    18901 ctatcgtgcg gagcgcaaaa gaatgcttat tgcaccgctg gtggcggttg ccactggaga
    18961 acgacattat caaagatggg cggattgttg atgtgcagcg gctggctaac acgttgttac
    19021 cgtggagtcg cgaactgccg cagcgtcatc acattatgtt ggcgtttccc gccagtcgca
    19081 cattacagcg gtcatttcca cgcccgtcga tgtccctcgg tgagcgggag caaatggcct
    19141 ggctgtccgg gacgatggcc cgtgaactgg atatggatcc ggactccctg cgcttcgatt
    19201 acagcgaaga ctcactcagt cccgcctata acgtgactgc cgcgcaaagc aaagagctgg
    19261 caacgctgct tactctggca gaaaggttgc gtgttcatgt tagcgcgatc actccggatg
    19321 ccagtgcatt acagcgattc ctgccttttt taccttctca tcagcaatgt ctggcctggc
    19381 gtgataacga acagtggctg tgggcgacgc gctatagctg ggggcgcaaa ctggcggtgg
    19441 ggatgactag cgcgaaggag ctggcggcag cgttatctgt tgatcccgaa agcgtcgcgc
    19501 tatgtggcga aggcggattt gacccctggg aggcggtttc tgttcgtcag ccgccgctac
    19561 cgccaccagg tggagacttt gccatcgcgc tggggctggc gcttgggaag gtgtactgat
    19621 gaacccgcca attaattttt tgccctggcg acagcaacgc cggaccgctt ttcttcgttt
    19681 ctggttgctg atgttcgttg cgcctttgct gctggccgtc gggataacgc taatactgcg
    19741 tctgacaagc aatgccgaag cacgcgtaaa cgccgttttg cttcaggcgg aacaacaact
    19801 cacccgcagc ttacagataa cgaaaccacg tttgctggaa cgccagcagt tacgcgaaca
    19861 gcgtttgcaa cgtcagcgcc agcgacaatt tacccgcgac tggcaatctg cgctggaagc
    19921 actagcggcg cttttacccg aacatgcctg gctgacaacg ataagctggc agcagggaac
    19981 gctggagatc aaggggctta caacaagcat taccgcgtta aacgcgctgg aaacgtcact
    20041 ccgccaggat gcttcttttc atctcaatca gcggggggcc acgcagcagg atgcgcaggg
    20101 acgctggcaa tttgagtatc agttaacaag gaaggttagc gatgaacatg ttctttgact
    20161 ggtggttcgc cacatcactc cgcctccgcc agctttgctg ggcagtctgg ttgctggtgt
    20221 tagttacgct catttttctg tcatcgacac accaggaaga gcgcgacgca ttaattcgac
    20281 tacgggcgag tcatcaccag cagtgggctg cactgtatcg cctggtagac gccactccct
    20341 tcagcgaaga aaaaacgctg cccttttcgc cactggattt tcagttatcc ggcgcgcaac
    20401 tggtttcctg gcatccatcc gcgcagggtg gtgagttggc gttgaaaacg ctgtgggaag
    20461 cagtgccgtc ggcatttaca cggctggcag agcgcaacgt cagcgtgagc cgtttttcgt
    20521 taagcgtgga aggtgatgac cttttgttca cgctacaact ggagacgccg catgagggtt
    20581 aaacgctggt tgttggcagg tattgcattg tgccttttaa ccggtatgcg tgaccctttt
    20641 aaaccgccgg aagatctatg ccggattagc gaacttagcc agtggcgcta tcaggggatg
    20701 gtagggcgag gcgagcgcat catcggtgta ataaaagatg ggcaaaaaaa atggcgacgg
    20761 gtgcagcaaa acgatgtgct ggaaaacggc tggacgatct tacagctgac gccagacgca
    20821 ctaacgctgg gaaccgggac aaactgcgaa ccgccacact ggttgtggca acggcaagga
    20881 gatacaaatg aagcaatgga tagccgcact actgttgatg ctgatacccg gcgtacaggc
    20941 ggcaaagccg caaaaagtga cgctgatggt ggatgacgtt ccggtagctc aggtgttgca
    21001 ggcgctggct gaacaggaga agttgaacct ggtggtttcg ccagacgtca gcggtacggt
    21061 gtcgttacat ttaactgacg ttccctggaa gcaggcacta caaactgtag tgaaaagcgc
    21121 cggattgata acgcgccagg agggcaacat tctctcggtg cattccgttg cctggcagaa
    21181 tgacaatatc gcccgccagg aggcggagca gacgcgggcg caggcaaatc tgccgctgga
    21241 aaatcgcaat attactctgc aatacgccga cgccggagag ctggcgaaag cgggggagaa
    21301 gctactgagt gccaaaggga gtatgaccgt cgataaacgc accaatcgcc ttttgctgcg
    21361 agataacaaa acggcgttaa gcacgcttga acagtgggta tcgcaaatgg atctgccggt
    21421 cgggcaggtt gagctgtcgg cgcatattgt caccattaat gaaaaaagtt tgcgtgagtt
    21481 aggtgtgaaa tggacgctgg ccgatgcgca acaagctggt ggcgttgggc aagtcaccac
    21541 gcttggcagc gacctctccg tagcgacggc gacaacgcat atcggtttta acattggacg
    21601 catcaacgga cgtttactgg atcttgagct ttccgcgctc gaacaaaaac agcagctgga
    21661 tattatcgcc agtccgcgtc tgctggcctc acatcttcag cctgccagca ttaaacaggg
    21721 gagcgaaatt ccatatcagg tttccagcgg ggaaagtggc gcgacgtcgg tggaatttaa
    21781 agaggccgtc ctggggatgg aagtcacgcc cacggtgtta caaaaaggtc gcatccggct
    21841 gaaattacac atcagccaga acgttccggg gcaggtgcta cagcaggcag atggcgaagt
    21901 gctggcgatt gataagcagg agatcgaaac gcaggtcgag gtcaaaagcg gagaaacgtt
    21961 ggcgctgggc ggcattttta cccgtaaaaa taaatcgggt caggatagcg taccgttgct
    22021 tggcgacatt ccctggttcg ggcaattatt tcgtcatgac ggaaaagaag atgaacgacg
    22081 cgagttagtg gtgtttatca cgccacgact ggtttccagt gagtaaacag ccgtaaaagc
    22141 ggtaatgttt ttacgctgaa cgtgtttcat ctatttgacg cgcgcaggta tttagcatac
    22201 aaggagtacc gatttgagag ttggtgctct tcgctgcctg cgttccatga tgatgattta
    22261 tcattcaggc ggcattttgc tgtctttttt acgctaatct tacccggtga tttatcgcca
    22321 gagcggtggt agcaaggcag cgcgcttgca gcgaccagat atgcagaggg atgggtgatt
    22381 tattcagttg ccaaacccgc tggagtattg agataatttt cagtctgact ctcgcaatat
    22441 cttatgaggt ttcagttcat gtcctgcggc gctctctgag cgaagcgggt ttatcattaa
    22501 cgaatagtct tagtagtacc gaaaaaatgg cagagaaacg caatatcttt ctggttgggc
    22561 ctatgggtgc cggaaaaagc actattgggc gccagttagc tcaacaactc aatatggaat
    22621 tttacgattc cgatcaagag attgagaaac gaaccggagc tgatgtgggc tgggttttcg
    22681 atttagaagg cgaagaaggc ttccgcgatc gcgaagaaaa ggtcatcaat gagttgaccg
    22741 agaaacaggg tattgtgctg gctactggcg gcggctctgt gaaatcccgt gaaacgcgta
    22801 accgtctttc cgctcgtggc gttgtcgttt atcttgaaac gaccatcgaa aagcaacttg
    22861 cacgcacgca gcgtgataaa aaacgcccgt tgctgcacgt tgaaacaccg ccgcgtgaag
    22921 ttctggaagc gttggccaat gaacgcaatc cgctgtatga agagattgcc gacgtgacca
    22981 ttcgtactga tgatcaaagc gctaaagtgg ttgcaaacca gattattcac atgctggaaa
    23041 gcaactaatt ctggctttat atacactcgt ctgcgggtac agtaattaag gtggatgtcg
    23101 cgttatggag aggattgtcg ttactctcgg ggaacgtagt tacccaatta ccatcgcatc
    23161 tggtttgttt aatgaaccag cttcattctt accgctgaaa tcgggcgagc aggtcatgtt
    23221 ggtcaccaac gaaaccctgg ctcctctgta tctcgataag gtccgcggcg tacttgaaca
    23281 ggcgggtgtt aacgtcgata gcgttatcct ccctgacggc gagcagtata aaagcctggc
    23341 tgtactcgat accgtcttta cggcattgtt acaaaagccg catggtcgcg atactactct
    23401 ggtggcgctt ggcggcggcg tagtgggtga tctgaccggc ttcgcggcgg cgagttatca
    23461 gcgcggtgta cgtttcattc aagtcccgac gacgttactg tcgcaggtcg attcctccgt
    23521 tggcggcaaa actgcggtca accatcccct cggtaaaaac atgattggcg cgttctacca
    23581 gcccgcttca gtggtggtgg atctcgactg tctgaaaacg cttcccccgc gtgagttagc
    23641 gtcggggctg gcagaagtca tcaaatacgg cattattctt gacggtgcgt tttttaactg
    23701 gctggaagag aatctggatg cgttgttgcg tctggacggt ccggcaatgg cgtactgtat
    23761 tcgccgttgt tgtgagctga aagcagaagt tgtcgccgcc gacgagcgcg aaaccgggtt
    23821 acgtgcttta ctgaatctgg gacacacctt tggtcattcc attgaagctg aaatggggta
    23881 tggcaattgg ttacatggtg aagcggtcgc tgcgggtatg gtgatggcgg cgcggacgtc
    23941 ggaacgtctc gggcggttta gttctgccga aacgcagcgt attataaccc tgctcacgcg
    24001 ggctgggtta ccggtcaatg ggccgcgcga aatgtccgcg caggcgtatt taccgcatat
    24061 gctgcgtgac aagaaagtcc ttgcgggaga gatgcgctta attcttccgt tggcaattgg
    24121 taagagtgaa gttcgcagcg gcgtttcgca cgagcttgtt cttaacgcca ttgccgattg
    24181 tcaatcagcg taacaacaag aaaggtcagg ccgcttatca agcggtctat tagcttcagg
    24241 ttaattgcaa cgtggtaagc attaaccttt tagtggggtg ttaaatggat gaattcaaac
    24301 cagaagacga gctgaaaccc gatcccagcg atcgtcgtac tggtcgttct cgtcaatctt
    24361 ctgaacgttc tgagcgtact gaacgtggcg aaccgcagat caattttgat gatattgaac
    24421 ttgatgacac tgacgatcgc cgtccgactc gtgcgcaaaa agcgcgtaat gaggaaccgg
    24481 aaatcgaaga agaaattgac gaatccgaag atgaaaccgt ggatgaagag cgcgtagagc
    24541 gtcgtccgcg taagcgtaaa aaagcagcca gtaaacccgc ttctcgtcag tatatgatga
    24601 tgggtgtcgg cattctggtt ctactgctgt tgatcatcgg tatcggttct gcgctaaaag
    24661 ccccctcgac ctcttccagc gatcaaactg cgtctggcga gaagagtatt gatcttgcag
    24721 gcaatgcgac cgatcaggcc aatggcgtgc agccagcgcc gggaaccacg tctgcggaaa
    24781 atactcagca ggatgtttct ctgccgccga tctcttctac gccgactcaa gggcaaaccc
    24841 cggcggcaac ggatggtcaa caacgtgttg aagtgcaggg tgacctgaac aatgcgctga
    24901 cccagccaca aaatcagcaa cagttgaaca atgtggcggt caattctaca ttgccgaccg
    24961 aacccgcaac ggttgcgcct gttcgcaatg gcaatgcatc gcgtgacacg gcgaaaacgc
    25021 aaaccgctga acgtccggcc actacgcgcc cagctcgtca gcaggcggtg attgaaccga
    25081 aaaaaccgca agcaaccgtg aaaacggaac cgaagccagt cgcacagacg ccgaagcgaa
    25141 ctgaaccagc tgcccctgtg gcgagcacaa aggcaccggc tgcgacttct acgccagcac
    25201 caaaagagac ggcgactacg gctccagtac agacggcatc cccggcgcaa accacggcaa
    25261 caccagccgc tggagggaag accgcaggta atgttggttc gttgaaatcg gcaccgtcca
    25321 gccattacac tctgcagctg agcagttcct ctaactacga caacctgaac ggttgggcga
    25381 agaaagagaa tctgaaaaac tacgttgtct atgaaacgac gcgtaatggt cagccgtggt
    25441 atgtcctggt ttctggcgtg tatgcttcga aagaagaggc gaaaaaagcg gtatctacat
    25501 tgccagcaga tgtccaggcc aaaaacccgt gggcgaaacc gctgcgtcag gtacaggccg
    25561 atctgaagta atcaaggtta tctcccgcaa tggtttatca ttgcgggagt tgcctgaagc
    25621 gctggatgct gtcggagctt tctccacagc cggagaaggt gtaattagtt agtcagcatg
    25681 aagaaaaatc gcgctttttt gaagtgggca gggggcaagt atcccctgct tgatgatatt
    25741 aaacggcatt tgcccaaggg cgaatgtctg gttgagcctt ttgtaggtgc cgggtcggtg
    25801 tttctcaaca ccgacttttc tcgttatatc cttgccgata tcaatagcga cctgatcagt
    25861 ctctataaca ttgtgaagat gcgtactgat gagtacgtac aggccgcacg cgagctattt
    25921 gttcccgaaa caaattgcgc cgaggtttac tatcagttcc gcgaagagtt caacaaaagc
    25981 caggatccgt tccgtcgggc ggtactgttt ttatatttga accgctacgg ttacaacggc
    26041 ctgtgtcgtt acaatctgcg cggtgagttt aacgtgccgt ttggccgcta caaaaaaccc
    26101 tatttcccgg aagcagagtt gtatcacttc gctgaaaaag cgcagaatgc ctttttctat
    26161 tgtgagtctt acgccgatag catggcgcgc gcagatgatg catctgtcgt ctattgcgat
    26221 ccgccttatg cgccgctgtc tgcgactgcc aactttacgg cgtatcacac aaacagtttt
    26281 acgcttgaac aacaagcgca tctggcggag atcgctgaag gtctggttga tcgccatatt
    26341 ccagtgctga tctccaatca cgatacgatg ttaacgcgtg agtggtatca gcgcgcaaaa
    26401 ttgcatgtcg tcaaagttcg acgcagtata agcagcaacg gcggcacacg taaaaaggtg
    26461 gacgaactgc tggctttgta caaaccagga gtcgtttcac ccgcgaaaaa ataattctca
    26521 aggagaagcg gatgaaacag tatttgattg ccccctcaat tctgtcggct gattttgccc
    26581 gcctgggtga agataccgca aaagccctgg cagcgggtgc agatgtcgtg cattttgacg
    26641 tcatggataa ccactatgtt cccaatctga cgattgggcc aatggtgctg aaatccttgc
    26701 gtaactacgg cattaccgcc cctatcgacg tacatctgat ggtgaaaccc gtcgatcgca
    26761 ttgtgcctga tttcgctgcc gctggtgcca gcatcattac ctttcatcca gaagcctccg
    26821 agcatgttga ccgcacgctg caactgatta aagaaaatgg ctgtaaagcg ggtctggtat
    26881 ttaacccggc gacaccattg agctatctgg attatgtaat ggacaagctg gatgtgatcc
    26941 tgctgatgtc tgttaaccct ggtttcggcg gtcagtcttt cattcctcaa acactggata
    27001 aactgcgcga agtacgtcgt cgtatcgacg agtctggctt tgacattcga ctggaagtgg
    27061 acggtggcgt gaaggtgaac aacattggcg aaatcgctgc ggcgggcgcg gatatgtttg
    27121 ttgctggttc ggcaatcttc gaccagccag actacaaaaa agtcattgat gaaatgcgca
    27181 gtgaactggc aaaggtaagt catgaataag tttgaagata ttcgcggcgt cgcttttgat
    27241 ctcgacggta cgctggttga cagtgcaccc ggtcttgctg ctgcggtaga tatggcgctg
    27301 tatgcgctgg agttgcctgt cgcaggtgaa gaacgcgtta ttacctggat tggtaacggc
    27361 gcagatattc tgatggagcg cgcattgacc tgggcgcgtc aggaacgtgc gactctgcgt
    27421 aaaacaatgg gtaaaccgcc cgttgatgac gacattccgg cagaagaaca ggtacgtatt
    27481 ctgcgtaaac tgttcgatcg ctactatggc gaggttgccg aagaggggac gtttttgttc
    27541 ccgcatgttg ccgatacgtt gggcgcgttg caggctaaag gcctgccgct aggcctggtc
    27601 accaacaaac cgacgccgtt cgtcgcgccg ctgctcgaag ccttagatat cgccaaatac
    27661 ttcagcgtgg tgattggtgg tgatgatgtg caaaacaaaa aaccgcatcc ggacccgctg
    27721 ttactggtgg ctgaacggat gggcattgct ccacaacaga tgctgtttgt tggcgactca
    27781 cgcaatgata ttcaggcagc aaaagcggca ggttgcccat cagttggctt aacctacgga
    27841 tataactacg gcgaggctat cgatctcagc cagcctgatg taatttatca gtctataaat
    27901 gaccttctgc ccgcattagg gcttccgcat agcgaaaatc aggaatcgaa aaatgactaa
    27961 gcccatcgtt tttagtggcg cacagccctc aggtgaattg accattggta actacatggg
    28021 tgcgctgcgt cagtgggtaa acatgcagga tgactaccat tgcatttact gtatcgttga
    28081 ccaacatgcg atcaccgtgc gccaggatgc acagaagctg cgtaaagcga cgctggatac
    28141 gctggcgttg tatctggctt gtggtatcga tcctgagaaa agcaccattt ttgttcagtc
    28201 ccacgtgccg gaacatgcgc agttaggctg ggcattgaac tgctatacct acttcggcga
    28261 actgagccgc atgacgcagt ttaaagataa atctgcgcgt tatgccgaga acatcaacgc
    28321 tggtctgttt gactatccgg tgctgatggc agcggacatc ctgctgtatc aaacaaatct
    28381 ggtaccggtg ggtgaagacc agaaacagca cctggaactg agccgcgata tcgcccagcg
    28441 tttcaacgcg ctgtacggcg atatctttaa ggtgccagag ccgtttattc cgaaatctgg
    28501 cgcgcgcgta atgtcgctgc tggagccgac caagaaaatg tccaagtctg acgataaccg
    28561 caataacgtt atcggcctgc tggaagatcc gaaatcggta gtgaagaaaa tcaaacgcgc
    28621 ggtcactgac tccgacgagc cgccagtagt tcgctacgat gtgcagaaca aagcgggcgt
    28681 ttccaacctg ctggatatcc tttctgcggt aacgggccag agcatcccgg aactggaaaa
    28741 acagttcgaa ggcaagatgt atggtcatct gaaaggtgaa gtggctgatg ccgtttccgg
    28801 tatgctgact gaattgcagg aacgttacca ccgtttccgc aacgatgaag ccttcctgca
    28861 acaggtgatg aaagacggcg cagaaaaagc cagcgcacac gcttcccgta cgctaaaagc
    28921 ggtgtacgaa gcgattggtt ttgtggcgaa gccgtaattt aacattaatg ccaaaaaaac
    28981 cggggaaacc cggttttttt atcctcattt gcaataatcc gaaaaaatgt gaagcgcctc
    29041 gccgtttcca catctcgctg ttgcgcctta tcttcaaatt catgaagata taaacattca
    29101 ttttattgaa aatatattat gcgtcgaacg tttatcaaaa aagaaggcgt cgttatcacg
    29161 acactggccc gttatttgtt gggtgaaaag tgcggtaatc gactgaaaac catagatgag
    29221 ctggcaactg aatgccgttc atcggttggc ctgacgcagg ccgcgttgaa aacgctggaa
    29281 tcaagcggag cgatacggat tgaacgccgt gggcgcaatg gcagttatct ggttgagatg
    29341 gataacaaag cattgctgac tcatgtggat atcaacaacg tggtatgtgc gatgcccttg
    29401 ccctataccc gtttgtacga aggcctggcg agcggattga aagcccagtt tgatggcatt
    29461 cctttttact atgcgcatat gcgcggcgcg gatattcgcg tggagtgttt acttaacggc
    29521 gtgtatgaca tggcggtggt ttcgcgactg gcggcggaaa gttatctcac gcaaaaagga
    29581 ttatgcctcg cgctggagtt gggaccgcat acctacgttg gcgagcacca gttgatttgc
    29641 cgtaaaggcg agtccgcaaa cgtgaagcgc gtggggctgg ataaccgttc ggcggatcag
    29701 aaaatcatga ccgatgtctt ttttggcgat agtgatgtgg aacgagtcga tctctcttat
    29761 cacgagagtt tacaacgcat tgttaaaggc gatgttgacg cggttatctg gaacgtagtg
    29821 gcggaaaacg aactgaccat gctgggatta gaagcgacgc cgctcacaga cgatccgcga
    29881 tttttacagg ccaccgaagc cgtggtcctg acgcgagtcg atgattaccc aatgcaacaa
    29941 ctgctgcgtg cagttgttga taaacatgcc ctgcttgctc atcaacagcg agtagtcagt
    30001 ggggaacagg aacccagtta ttaaacgaaa ggcatctgat atggaaacca gactcaacct
    30061 gctttgcgat gcaggcgtta ttgataagga catctgcaaa ggcatgatgc aggtcgtcaa
    30121 tgtactggaa acagagtgcc atctaccggt gcgcagtgag caaggaacga tggcgatgac
    30181 acatatggcg agtgcactga tgcgcagtcg ccgtggtgaa gaaatagagc cgctggatga
    30241 cgaattgctg gcggaactgg cgcaatccag ccactggcaa gccgttgtgc aattgcatca
    30301 ggtattgttg aaggaatttg cactggaagt taacccgtgt gaagaaggct atttgctggc
    30361 caacctttat ggattatgga tggctgctaa cgaagaggtt tgaagctccc gataaatcca
    30421 cgtcgtacaa cctaaatatt caaaaaaatg aatatttaat tcaaggaaca gaaacgaggc
    30481 aacaagatgt ttgtagaagc attgaaacgc cagaacccgg cgctgatttc cgccgcacta
    30541 agtttgtggc agcagggcaa gatcgccccg gacagttggg tgatcgacgt ggatcaagtt
    30601 ctggaaaacg gcaagcggct gattgagacg gcgcggcttt acggcattga actgtatctg
    30661 atgaccaaac aattcggtcg taatccgtgg ctggcagaaa aactgctggc tctgggctac
    30721 agcggcattg tggcggtgga ttacaaagag gcgcgagtca tgcgccgcgc cggtttgccg
    30781 gtagcgcatc aggggcatct ggtacaaatc ccttgtcatc aggtttctga cgccgttgaa
    30841 cagggaaccg acgtcatcac tgtgtttacc ctcgacaaag cgcgggaaat ttctgcggca
    30901 gcggtgaagg ccggacgtgt gcagtctgtg ctgctcaaag tttatagcga cgatgatttt
    30961 ctttatccgg ggcaggagag cggttttgta cagcattcgc tgcacgaggt ggttgctgaa
    31021 atccagaatc tgccggggct acatttggcg ggacttaccc atttcccttg cctgctttgg
    31081 gatgaggctg ccgggaaagt tttgccgaca ccgaatcttc acacgctggt acaagcacgg
    31141 gatcaactgg cgaaatctgg tattgcgatt gagcaactga acgcgccttc agcgaccagc
    31201 tgcacgtcgc tgccattgct ggtggaatac ggtgtgactc ataccgaacc cggtcatgcg
    31261 ctgacgggca ctattccggc aaaccagcgg ggcgatcagc ctgaacgtat cgcgatgctc
    31321 tggttaagtg aaatctccca tcatttccgt ggcgacagct actgctacgg cggcggttac
    31381 taccgtcgtg gtcacgcgca acatgcgctg gtgtttacgc cagaaaatca aagaattact
    31441 gaaacatatc tcaacgccgt ggatgacagc agtatcgact acaccctgcc gctggcaggc
    31501 gagcatccgg taagcagtgc agtggtgctc tgttttcgca cgcagatttt tatcacccgt
    31561 agcgatgtgg tgttggtgtc cggtattcac catggcgaac cagaaatagt tggtcgttat
    31621 gacagcctcg gcaaccctct ggaggcgtaa atggcgcgat ttgtggtgtt agtgattgat
    31681 agctttggcg taggggcaat gaaagatgtc acgttggtgc gtccgcagga tgccggggcg
    31741 aatacctgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagacg
    31801 ctggggctaa tcaacgcatt gggttatgcg cctggcgata tgcagccgtc agactccgca
    31861 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    31921 attttaggta cgcgcccatt accgccgctg cggatgccct ttcgtgatgt gattgaccgt
    31981 gttgagcagg cattagtttc cgctggctgg caggttgagc gtcgtggcga tgatctgcaa
    32041 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggtcag
    32101 gtctataaca tcaccgccaa tctctctgtg atctcttttg acgacgcaat caaaatgggt
    32161 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    32221 gacagccagt gcatcctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    32281 gcgccgcgtt ctggcgccta tgacaacggt ttccaggtcg tacatatggg ctacggtgtc
    32341 gatgaaaaag tgcaggtgcc gcaaaaactg tatgaagcgg gcgtgccaac cgtgctggtg
    32401 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    32461 agccagcgga ttatggatat caccctcaac gaatttaaca cctatccgac ggcgtttatt
    32521 tgtaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    32581 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgaggcgat gcagccagat
    32641 gattgcctgg tcgtgatggc ggatcacggt aacgatccga ccatcggtca cagccaccat
    32701 acccgcgaag tggtgccagt gctggtttat cagcaaggac tggtcgctac gcagctcggt
    32761 gtgcgcacca cgctttctga tgtgggggct accgtgtgtg aatttttccg tgcgccaccg
    32821 ccacaaaatg gtcgctcttt tctttcctcc ttccggtttg caggagacac cctatgagtt
    32881 ttgatccgac gggctacacc ctcgcccatg agcatctgca tattgatctt tccggcttta
    32941 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    33001 tgatggcccg gggcgtgcgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    33061 cgcaatttat gctcgatgta atgcgcgaaa ccgggatcaa cgtggtgggc tgtaccggtt
    33121 attaccagga cgcgtttttc ccggaacatg tggcgacccg cagcgtgcag gaactggcgc
    33181 aggagatggt cgatgaaatt gagcagggta tcgatggcac tgacctgaaa gccggaatta
    33241 tcgcggagat cggctccagc gaagggaaga ttacgtcgct ggaagagaag gtgtttattg
    33301 ccgccgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    33361 cgatggggct ggagcaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    33421 ccgtcggcca ctgcgatctg aaagacaatc tcgacaacat tttgaagatg atcgatctcg
    33481 gcgcgtatgt gcagttcgac accatcggca agaacagtta ctacccggac gaaaagcgca
    33541 ttgcgatgct ccatgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    33601 atattacgcg gcgctctcat ttaaaagcca acggtggtta tggctatgac tttttattga
    33661 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    33721 tacgtgaaaa tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    33781 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc tggctgtttt
    33841 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg acaactggat
    33901 tactacatcg gcgcgtgtaa taccggtgcg ggcgcagcct tgtcgattgc tatcgcggtg
    33961 attggctata acaaaagttg caccattgcc aagccgggca ttaaagcgaa agacgaacat
    34021 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgttgaa
    34081 cacgcgattc cgatgctgat taaccatcta aaataaaagg cacgactatg gatctgtata
    34141 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    34201 cggccgtttt tcatgacggt atccgcccga tcctgccaca actgattgaa ggctatatga
    34261 accgtcgcga ggcggggagc atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    34321 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    34381 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggt gctatctggg
    34441 gaatattgat cctcacctgt ctgttgccag taaaccagct gctgaccgcg ctaccggtgg
    34501 atgtattagg tagcctgggg gaattaagct cgccggtggt ttcagctttt gcactgttcc
    34561 cgttggtggc gattttctac cagtttggct ggaagcaaag tctgatcgcc gccgtggtgg
    34621 tactgatgac tcgtgtggtg gtagtacgct atttcccaca tcttaaccct gaatccatcg
    34681 aaatctttat tggcatggtg atgctgctgg ggatcgcaat aactcatgac ctgcgtcatc
    34741 gtgatgaaaa tgatatcgat gccagcggta tgtcggtgtt tgaagagcgt acatcacgga
    34801 ttatcaaaaa cttaccgtat atcgccatcg tgggtgcatt gattgctgcc gttgccagca
    34861 tgaagatttt cgccggtagt gaagtgtcga tcttcacact ggagaaagcg tactccgcag
    34921 gcgtaacgcc ggaacaatcg caaacactaa tcaaccaggc ggcactggca gaatttatgc
    34981 gcgggctggg ttttgtgccg ttgattgcca ccacggcgtt agccaccggc gtgtatgcag
    35041 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atggttgcag
    35101 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttct atcggcaaat
    35161 ggttggggcg ctacccgtcg gtacgtaatg cgtcggataa catccgtaac gccatgaata
    35221 tgctgatgga agtggcgttg ctggtcggtt cgatctttgc agcaatcaaa atggcgggct
    35281 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    35341 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggcatcctg ctgaatgttc
    35401 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    35461 gcaaagcctg acgcttattg aggcgcagca aaagcagttt gcgctggtgg acacgatttg
    35521 ccgtcatttt cctggcagcg agtttcttac ctgcggtgat ttgggcttaa cgccggggct
    35581 aaatcagccc cgcattaccc agcgtgtgga acaggtgctg gctgacgcgt ttcacgcaca
    35641 ggccgcggcg ctggtacagg gcgcgggtac tggcgcgatc cgtgcggcgc tggcagcttt
    35701 gctcaaatcg gggcagcgtc ttctggtgca tgacgcgccg gtttatccaa cgacgcgcgt
    35761 cattattgag cagatggggc taacgctcat tacggctgat ttcaatgatc tgtcggcact
    35821 gaagcaggtt gttgacgagc aacaacctga tgccgcgctg gtgcagcata cgcgccagca
    35881 gccgcaggac cgctatgtgc tggcagacgt gctggcaacg ttgcgcgcgg caggtgttcc
    35941 agcgttaacc gatgacaact atgcggtgat gaaggtagcg cgaatcggct gcgaatgcgg
    36001 ggcgaatgtg tcgacatttt cctgcttcaa gctatttggg ccagagggcg ttggtgcggt
    36061 ggtaggcgat gccgatgtta ttagccggat tcgcgccacg ctctattccg gcggcagtca
    36121 gattcagggg gcgcaggcat tagaggtgtt gcgtggtctg gtgctcgcgc cagtgatgca
    36181 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggcg gcgcggtggc
    36241 ggaagtgaaa agcgccgtca ttgccaatgc acagtcgaag gtattgattg tggagtttca
    36301 tcagccgatt gccgccagag tgctggaaga ggcgcagaaa ctcggtgcct tgccttaccc
    36361 ggttggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgacttt ccggaacgtt
    36421 tcgccaggcg aatccgcagt tagaacattg cgcgattcgc attaacccaa atcgcggcgg
    36481 tgaagagacg atattgcgga tcttgcgtga aagtattgcc agcatttaat cgttgtaaac
    36541 gatattttcg aaaagaatgc agaaacaaat acgccctcgg ataactccga gggcgtcagg
    36601 caattaaacc ggcgttagcc gatttctgtc agagacttac tgtgcagtcg gaccgcaacc
    36661 gccaatgatt ttagaaatag aaattgccgg gtgcagcagg tagtcgtaag agcagttctt
    36721 atcacggttt tcaatatgac cagtacatgc agttaacgta gccagtactg cg
//
LOCUS       DACADA010000030        36562 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.030, whole genome shotgun sequence.
ACCESSION   DACADA010000030 DACADA010000000
VERSION     DACADA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.030"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(81..782)
                     /locus_tag="ISB17_001494"
     CDS             complement(81..782)
                     /locus_tag="ISB17_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HAP1979438.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSDGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGAMK"
     gene            complement(779..1708)
                     /locus_tag="ISB17_001495"
     CDS             complement(779..1708)
                     /locus_tag="ISB17_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1979439.1"
                     /translation="MANTAEIFNFPVPDAAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGIFIQS
                     GRNTGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGVYPNQVNTKDKL
                     TKDNIKPFSSENSGESSDQPENDLPVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(1795..2334)
                     /locus_tag="ISB17_001496"
     CDS             complement(1795..2334)
                     /locus_tag="ISB17_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAP1979440.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYLEQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2404..2634)
                     /locus_tag="ISB17_001497"
     CDS             complement(2404..2634)
                     /locus_tag="ISB17_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979441.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            2673..3428
                     /locus_tag="ISB17_001498"
     CDS             2673..3428
                     /locus_tag="ISB17_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1979442.1"
                     /translation="MVVFSQQPFSFDGIKPWLYIWTMKTTLSERLKEARLARGLTQKA
                     LGDLVGVSQAAIQKIETGKANQTTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQST
                     VSHSKYFKIDVLDIEVSAGPGVINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINV
                     RGDSMSGTIEPGDLLFVDITVKSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKS
                     YSPWDPIEKDEMNRVFIFGKVIGSMPQTYRKHG"
     gene            4024..4230
                     /locus_tag="ISB17_001499"
     CDS             4024..4230
                     /locus_tag="ISB17_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HAP1979443.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4306..4602
                     /locus_tag="ISB17_001500"
     CDS             4306..4602
                     /locus_tag="ISB17_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAP1979444.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHHVDIDSEV"
     gene            4608..5393
                     /gene="bet"
                     /locus_tag="ISB17_001501"
     CDS             4608..5393
                     /gene="bet"
                     /locus_tag="ISB17_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HAP1979445.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            5390..6070
                     /locus_tag="ISB17_001502"
     CDS             5390..6070
                     /locus_tag="ISB17_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HAP1979446.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6067..6249
                     /locus_tag="ISB17_001503"
     CDS             6067..6249
                     /locus_tag="ISB17_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HAP1979447.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            6222..6413
                     /locus_tag="ISB17_001504"
     CDS             6222..6413
                     /locus_tag="ISB17_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HAP1979448.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            6424..6705
                     /locus_tag="ISB17_001505"
     CDS             6424..6705
                     /locus_tag="ISB17_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP1979449.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            6804..7025
                     /locus_tag="ISB17_001506"
     CDS             6804..7025
                     /locus_tag="ISB17_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HAP1979450.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            7025..7351
                     /locus_tag="ISB17_001507"
     CDS             7025..7351
                     /locus_tag="ISB17_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP1979451.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            7335..7574
                     /locus_tag="ISB17_001508"
     CDS             7335..7574
                     /locus_tag="ISB17_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAP1979452.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            7714..7950
                     /locus_tag="ISB17_001509"
     CDS             7714..7950
                     /locus_tag="ISB17_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP1979453.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            7940..9082
                     /locus_tag="ISB17_001510"
     CDS             7940..9082
                     /locus_tag="ISB17_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1979454.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(9196..10446)
                     /gene="icd"
                     /locus_tag="ISB17_001511"
     CDS             complement(9196..10446)
                     /gene="icd"
                     /locus_tag="ISB17_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAP1979455.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            10618..11271
                     /gene="rluE"
                     /locus_tag="ISB17_001512"
     CDS             10618..11271
                     /gene="rluE"
                     /locus_tag="ISB17_001512"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAP1979456.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            11281..11742
                     /gene="nudJ"
                     /locus_tag="ISB17_001513"
     CDS             11281..11742
                     /gene="nudJ"
                     /locus_tag="ISB17_001513"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAP1979457.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            11796..12902
                     /gene="mnmA"
                     /locus_tag="ISB17_001514"
     CDS             11796..12902
                     /gene="mnmA"
                     /locus_tag="ISB17_001514"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP1979458.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            12938..13579
                     /gene="hflD"
                     /locus_tag="ISB17_001515"
     CDS             12938..13579
                     /gene="hflD"
                     /locus_tag="ISB17_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAP1979459.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            13583..14953
                     /gene="purB"
                     /locus_tag="ISB17_001516"
     CDS             13583..14953
                     /gene="purB"
                     /locus_tag="ISB17_001516"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP1979460.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            15122..15793
                     /gene="phoP"
                     /locus_tag="ISB17_001517"
     CDS             15122..15793
                     /gene="phoP"
                     /locus_tag="ISB17_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAP1979461.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            15793..17253
                     /gene="phoQ"
                     /locus_tag="ISB17_001518"
     CDS             15793..17253
                     /gene="phoQ"
                     /locus_tag="ISB17_001518"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAP1979462.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            17329..18450
                     /locus_tag="ISB17_001519"
     CDS             17329..18450
                     /locus_tag="ISB17_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP1979463.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18499..19725)
                     /gene="pepT"
                     /locus_tag="ISB17_001520"
     CDS             complement(18499..19725)
                     /gene="pepT"
                     /locus_tag="ISB17_001520"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP1979464.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            19975..21111
                     /gene="potA"
                     /locus_tag="ISB17_001521"
     CDS             19975..21111
                     /gene="potA"
                     /locus_tag="ISB17_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAP1979465.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            21095..21952
                     /gene="potB"
                     /locus_tag="ISB17_001522"
     CDS             21095..21952
                     /gene="potB"
                     /locus_tag="ISB17_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAP1979466.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFIFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            21949..22743
                     /gene="potC"
                     /locus_tag="ISB17_001523"
     CDS             21949..22743
                     /gene="potC"
                     /locus_tag="ISB17_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAP1979467.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            22740..23786
                     /gene="potD"
                     /locus_tag="ISB17_001524"
     CDS             22740..23786
                     /gene="potD"
                     /locus_tag="ISB17_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAP1979468.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            23844..24305
                     /locus_tag="ISB17_001525"
     CDS             23844..24305
                     /locus_tag="ISB17_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HAP1979469.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            24302..25090
                     /locus_tag="ISB17_001526"
     CDS             24302..25090
                     /locus_tag="ISB17_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP1979470.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(25228..26049)
                     /gene="cobB"
                     /locus_tag="ISB17_001527"
     CDS             complement(25228..26049)
                     /gene="cobB"
                     /locus_tag="ISB17_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP1979471.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(26065..26976)
                     /gene="nagK"
                     /locus_tag="ISB17_001528"
     CDS             complement(26065..26976)
                     /gene="nagK"
                     /locus_tag="ISB17_001528"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAP1979472.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(27005..28249)
                     /gene="lolE"
                     /locus_tag="ISB17_001529"
     CDS             complement(27005..28249)
                     /gene="lolE"
                     /locus_tag="ISB17_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAP1979473.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(28249..28950)
                     /gene="lolD"
                     /locus_tag="ISB17_001530"
     CDS             complement(28249..28950)
                     /gene="lolD"
                     /locus_tag="ISB17_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAP1979474.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(28943..30142)
                     /gene="lolC"
                     /locus_tag="ISB17_001531"
     CDS             complement(28943..30142)
                     /gene="lolC"
                     /locus_tag="ISB17_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAP1979475.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            30403..31476
                     /locus_tag="ISB17_001532"
     CDS             30403..31476
                     /locus_tag="ISB17_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP1979476.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            31603..35049
                     /gene="mfd"
                     /locus_tag="ISB17_001533"
     CDS             31603..35049
                     /gene="mfd"
                     /locus_tag="ISB17_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP1979477.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            35192..36154
                     /gene="ldtC"
                     /locus_tag="ISB17_001534"
     CDS             35192..36154
                     /gene="ldtC"
                     /locus_tag="ISB17_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAP1979478.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(36237..36494)
                     /gene="bhsA"
                     /locus_tag="ISB17_001535"
     CDS             complement(36237..36494)
                     /gene="bhsA"
                     /locus_tag="ISB17_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAP1979479.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9160 a         9297 c         9123 g         8982 t
ORIGIN      
        1 aatcaatcat ttcgggcgtt acaactagtc ctagattata ttcgctaggt atactttatt
       61 tttaagctaa tactttagct ctatttcatc gctcctttca gcccgaactt agctttgatt
      121 tctgcgatct tcgccagagc ctgtgcacga tttagaggcc taccgcccat gacaggaagt
      181 tgttttactg gttcaggtat agcctcacca cggttaattc gcgcggtcat acaggccagt
      241 tcatcggcag ccttgcgccg taattccgcg tcagtcaacg cattggcccg catgttctga
      301 tacaggttgg taaccagcca gtagtgcgcg tttgatttcc acggataaga ctctgcatcc
      361 ggatacagac cacgcttccg gcaatactcg taaaccatat caaccagctc gctgacgttt
      421 ggcagcccgg cggtaacgga tgcttcttcc cggcaccatg caacaaactg cccgggtgat
      481 ggcagaaatg gtctattctg ccgacgggca acacgcattc cgtcgctcac ctgctccatc
      541 gtggtgatcc cgttttcccg gaaagccaga acccactggc ggcggatttc gttcagttcg
      601 ttctggtcac gattagccag gctcgccggg aaagttgcca gtaactggct gaatacaccg
      661 ttgattatct gcgctacctg ctgtacctgc ggcttttcgt cgtactgttc cggcatgttg
      721 ttggcgatcc gacgcatctg ctcacggtca aagttaatca tctgtgcggc gatgtttttc
      781 atagatccac cccgtaaatc cagtctgtgt ttgtcaggtc gagttttggt ttgctagctg
      841 tcacgcctgc ctgttgcttg ttacggttga tttcgagttg ggtccacttg tcgcggagtt
      901 tggccggact tagcacgtta ccggaccaga agttgtcctg gcatgcccag cggaacagca
      961 cgcacatgtc gcggtggtta cgtccgtcac gttcacgcat caggcggata tcgttagccc
     1021 accctgcaaa attcggtttt ctggctgatg gcgcgatggt cttcaccatg tcaaacatcc
     1081 actctgcggc ggtcaggtct tctgctgtcc cccacttgct gccgctctga attgcagcat
     1141 ccggtttcac cacaggaaga tcgttttctg gttggtcaga ggattcgcca gaattctcgg
     1201 acgaaaaagg ttttatattg tcttttgtta gtttgtcttt tgtgtttacc tgattcgggt
     1261 aaacgccttt acctgatttg ggtaaacttt ttttacctga ttcaggtaaa tttacctctt
     1321 tcaggtaaac tttatttttc ttacctgatt cgggtaatgt tgaccattca ctgaccacat
     1381 tattaatgcc ggtattccgc ccgctctgaa taaaaatccc acgctttacc agaacacttt
     1441 ttgcagcaga acacttgtgc ggcaatatcc cggttaattc ggaaagttgc tcgttgctaa
     1501 cccaatccag ttttttatta aagccatatg ttttgcgcat gacagccaga aagaccagaa
     1561 gctggtgctg tgttaatccg gccagcatca cagcttccag caactcattt gcaatgcgcg
     1621 tataaccatc atcgagatct gccacgcgcg gctccttttg tgccgcatcc ggcactggaa
     1681 aattgaatat ctcagcagtg tttgccataa ttcctcccgc aatgagtgtg ttacgatttg
     1741 cacctgaaag tcggttctgt tccagcagac cggctttcgc catttctgaa cctgtcatat
     1801 cgcccccagc atggtagtaa ccatcgccat caatggacca gccagatctg ggtccacacg
     1861 aaacatcgac acaatacctt cactaatttc cttcagtttc tggtggcgtg gtgcgttgag
     1921 aatgacagcc tgttttgcct cactgagttc cttttccatt tcagccaacc tagccatgaa
     1981 gctatcctgc tcaaccaggt aaccgcgata ttccagcggt agtaccgcca gaattgccgg
     2041 ggtcagttca cgcacgttat ttcggtattt ttcagaatcg aatttgttat cgaggaagcg
     2101 gaacagcttc tggcgtgcac ggctgacatc atcagggaaa tcgatggtgc cgccgccctg
     2161 ctccagatac tcattcacaa tgagtgtggc aacgacatcc tgattatcta cagccgacca
     2221 ggcgcggacg gcatcacgga ttttttcgtg gcctggcacc tgttttgttt gagaacgatt
     2281 tatcaccgca gtcgggctaa atccgctagt ctgttggtat gtaagtggtt gcataattga
     2341 ctcctttagt ttgaattgac tgttaagttg attgcttatt gttaaagagc gtgaaatgga
     2401 aatttaagct gcgttctttt cggtgtgtgg aaacaacttc ggaagatccg ggcgaatctg
     2461 gtatgccttc actactccac cagtagccgt aacaatgctg ccgacatgtt caggggatac
     2521 ctttgctttg ttgtgaagcc acttatagac ggcctgctgt gaaacttcgc aagcagcgcc
     2581 cagtttcttt tgtgaaccaa cgatattgat cgctgttttg atagctgggt tcataacaac
     2641 ctccgtggtt aatttgaatc aagattaaaa ctatggttgt ttttagtcaa caaccatttt
     2701 cgtttgatgg aataaaacct tggttgtaca tttggactat gaaaacaaca ctctcagaaa
     2761 gacttaaaga agccagatta gcgcgaggcc ttacacaaaa ggcgcttggg gatttggtcg
     2821 gggttagcca ggctgctatt cagaaaatcg aaacagggaa agctaatcaa acaactaaaa
     2881 tcgtggagat cgcgaacgct ttgggtgtgc gcgcagaatg gttatcttct ggcgttggaa
     2941 atatgtcaga cagtacagtg caaccaatac aatcaactgt cagccattcc aaatacttca
     3001 agattgacgt tcttgatata gaagtcagtg ctgggccggg agtcatcaac cgtgagtttg
     3061 tagaagttct acgctcggtt gagtactcgt ttgacgatgc tcgtcacatg ttcgatggta
     3121 ggaaggcgga aaatatccgc atcattaacg tgcgtggtga cagcatgtca ggaacgatcg
     3181 aaccaggtga tctgctgttc gttgatatca cagttaaatc tttcgacggt gatggtatct
     3241 atgcgtttct gtacgacgac acagcccatg taaagcgcct gcaaatgatg aaggataagc
     3301 tgctggtcat ctctgataac aaaagctact caccgtggga cccgatcgag aaagacgaga
     3361 tgaaccgggt gttcatcttc ggtaaggtta ttgggagcat gccgcagaca tataggaagc
     3421 atgggtagta ccaattaaaa attatcaact gggcattgtg ctcattcagt aaagaactaa
     3481 ttcctatctt tgctctaggt agtaatatta agccaccgca ataatatctt tacctaacgg
     3541 cgtaagaatc ccggtcaccg tgccgggttt tcttttgccc tcccctcatc acacacaccg
     3601 ttaaaaaaac caccataacc tcgcttcagt tatcgctatg cgattcaagt cacaaaataa
     3661 atccatccta aatacaacca gttatatcta aaacaaccaa taaaacaact tttgttgttg
     3721 acgataaaac aactatagtt ttaaataagt tcatcgcaac aacacaacga tacggcaact
     3781 acctgattca ccgttgcgat gaccgcttag atccgcagtt tgaatttcag caggcttcgg
     3841 ggagtgcgag gggtgaaacg gacgcgtgaa cgtcggtgtg accagctgaa attaactcaa
     3901 catttcatac cttagtcgct tcaacgaggc ggcttagtta tgacaaccgg cggccatcca
     3961 ccgcctgaat acgcgcagaa gtctctatat gttcagcagc ccagcttacg ggcaggagtt
     4021 tttatggttc atcaacatta cggaacgcag accgttaatc gcggtgcggt catgccagga
     4081 atgctggtca aacacaaaga tggtacctgg actgcatcag ctaatttacg cggacggctt
     4141 tatctgcatc gcggcatcga gcgcacttat acccgtgatt tgctcgtgga agtttttctc
     4201 gacggacgcg gcaacggcct gaatcactaa tcccctttcc tgtttaccta atcagcccgg
     4261 catttcgcag gcgatatttt cacagccatt ttcaggagtt cagccatgaa cgcttattac
     4321 attcaggatc gtcttgaggc tcaaagctgg gcgcgtcact accagcagat cgcccgtgaa
     4381 gagaaagagg cagaactggc agacgacatg gaaaaaggtc ttccacagca cctgtttgaa
     4441 tcactctgca tcgatcattt gcaacgctgc ggggccagca aaaaagccat tacccgtgcg
     4501 tttgatgacg atgttgagtt tcaggagcgc atggcagaac acatccggta catggttgaa
     4561 accattgctc accaccatgt tgatattgat tcagaggtat aaaacggatg agtacagcac
     4621 tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat ggattctgtc gacccacagg
     4681 aactgatcac cactcttcgt cagacggcat ttaaaggtga tgccagcgat gcgcagttca
     4741 tcgcattgct gatcgtcgcc aaccagtacg gccttaatcc gtggacgaaa gaaatttacg
     4801 ccttccctga taagcagaac ggcatcgttc cggtggtggg cgttgatggc tggtcccgca
     4861 tcatcaatga aaaccagcag tttgatggca tggactttga gcaggacaat gaatcatgta
     4921 cgtgccggat ttaccgcaaa gaccgcaatc atccgatctg cgttaccgaa tggatggatg
     4981 aatgccgccg cgaaccattc aaaactcgcg aaggcagaga aatcacgggg ccgtggcagt
     5041 cgcatcccaa acggatgtta cggcataaag ccatgattca gtgtgcccgt ctggccttcg
     5101 gatttgctgg tatctatgac aaggatgaag ccgagcgcat tgtcgaaaat actgcataca
     5161 ctgcggaacg tcagccggaa cgcgacatca ctccggttaa cgatgaaacc atgcaggaga
     5221 ttaacactct gctgattgcc ctggacaaaa catgggatga cgacttattg ccgctctgtt
     5281 cccagatatt tcgccgcgac attcgcgcat cgtcagaact gacacaggcc gaagcagtga
     5341 aagctcttgg attcctgaaa cagaaagcca ctgagcagaa ggtggcagca tgacaccgga
     5401 cattatcctg cagcgtaccg ggatcgacgt gagagctgtc gaacaggggg atgatgcatg
     5461 gcacaaatta cggctcggcg tcatcaccgc ttcagaagtt cacaacgtga tagcaaagcc
     5521 ccgctcagga aagaagtggc ctgacatgaa aatgtcctac ttccacaccc tgctggctga
     5581 ggtttgcacc ggtgtggctc cggaagttaa tgctaaggcg ctggcgtggg gaaaacagta
     5641 cgagaacgac gccagaaccc tgtttgaatt cacttccggc gtgaatgtta ctgaatcccc
     5701 gatcatctat cgcgacgaaa gtatgcgcac cgcctgctct cccgatggtt tatgcagtga
     5761 cggcaatggc cttgagctga aatgcccgtt tacctcccgg gatttcatga agttccggct
     5821 cggtggtttc gaggccataa agtcggctta catggcccag gtgcagtaca gcatgtgggt
     5881 gacacgaaaa gatgcctggt actttgccaa ctatgaccca cgaatgaagc gtgaaggcct
     5941 gcattatgtc gtggttgagc gggatgaaaa gtacatggcg agttttgacg agatggtgcc
     6001 ggagttcatc gaaaaaatgg acgaagcact ggctgaaatt ggttttgtat ttggagagca
     6061 atggcgatga cccatcctca cgataatatc cgggtaggcg cgatcacttt cgtctactcc
     6121 gttacaaagc gaggctgggt atttcacggt ctttctgtta tccgaaatcc gctgaaagcc
     6181 cagcggctgg ctgaggagat aaataataaa cggggggcgg tatgcacaaa gcatctcctg
     6241 ttgagttaag aacgagtatt gatttggcac atagccttgc tcaaattgga gtcaggtttg
     6301 tgccaatacc agcagaaaca gacgaagaat ttcatacgtt agccacatcc ctttcacaaa
     6361 agctggaaat gatggtggcg aaagcagaag cagatgagag agaccaggta tgacaaccac
     6421 tgaatgcatt tttctggcag cgggcttcat attctgtgtg cttatgcttg ccgacatggg
     6481 gcttgttcag tgacacctca gcaagaaaat gcccttcgca gcattgcccg tcaggctaat
     6541 tctgaaatca aaaaagtcag acagcagttt ccggataaaa acgtcaatga catttgccgt
     6601 agcgtactga agaagcaccg cgaaacggta acgctgaagg gattcacacc gactcattta
     6661 agcctggcaa tcggcatgtt aaacggcgtc tttaaggaac gatgaacatg aaaagcaaaa
     6721 ttatcaggga gctacaggct ccttttttat tgttcgcatt caccctcaag cgtattaacc
     6781 aacagttcag ggattaatga aagatggcag acatcattga ttcagcatca gaaatagaag
     6841 aattacagcg caacacagca ataaaaatgc gccgcctgaa ctacctggct gtatctgcca
     6901 ctcattgttg tgagtgtggc gatccgatag atgaacgaag acgcctggcc gttcagggtt
     6961 gtcggacttg cgccagttgc cagcaagatc tggagcttat ccgcaaacaa agggggatta
     7021 agtgatggct aacctgcaac ttgctgttaa aagtgaatac ttcgatgcca tgattcgcgg
     7081 agagaaaacg gaagagtatc gcttgtgtaa tgactactgg aataagcgaa ttatgttccg
     7141 ggaatatgac cgcctgatta tcacaaaggg atatccgaag cgcgacgatt ccagtcgcag
     7201 aattgatgtt ccgtatgacg gatatgaagt gaagacaatc acacatccgc actttggcga
     7261 taaaccggta aaggtgttcg ctataaaggt gaatatcagc actgaatatc aatccgcaca
     7321 acacaaggtc aagaatgttc agagtgattg accctaacac ctggtacgtc gaccaccacg
     7381 gcactccctg caaaatcctg cgttctaccc acaacaaagt tcactacatc cgaaaaggca
     7441 gaacatgtat cgccagcatg ttccgcttta atcatgactt tgaacctgtg aataaagctg
     7501 atgcagatcg gatagcagaa gagatcgaaa cggcagaaca cattaagaag ttacgtgaca
     7561 tgcgcaggaa atagaaaaat tgataaattc aatactgcat ttctcagcat taaatttatc
     7621 tctatgacca gtcaagagat gtacctgcca tgagcttaat atcatgtcag atatatcggt
     7681 cacaaactcc ctcagcagct aagaggagga caaatgtctc gactaatcac tttacaggac
     7741 tgggctaaag aagaatttgg ggacttagca ccaagtgagc gagttctgaa aaaatacgcg
     7801 caagggaaaa tgatggcccc acccgctata aaagttggtc gctactggat gattgaccga
     7861 aattcccgtt ttgtaggaac gcttgcagaa ccgcaactcc caataaacgc aaacccaaaa
     7921 ctccaaagga taatcgctga tggctgctag accccgatct cacaaaatct ctatacccaa
     7981 tttatattgc aaattagata agcgaaccgg aaaggtatat tggcaataca aacatccact
     8041 atccggtcgt tttcatagct taggaactga tgagaatgaa gcaaaacaag ttgctactga
     8101 agcaaatacc attattgctg aacaacgtac cagacaaata ttaagcgtca atgagcgtct
     8161 ggaaagaatg aaaggcaggc gctcagacat tacggtgaca gaatggcttg ataaatatat
     8221 ttctatccaa gaggacaggc tgcaacataa tgaactaaga cccaactcct atcggcaaaa
     8281 aggcaaaccc attcgtcttt tccgtgagca ttgtggaatg caacacctca aggatattac
     8341 cgcacttgat attgccgaaa taattgatgc tgtaaaggct gaaggtcata acaggatggc
     8401 gcaagtcgtg agaatggtgt tgatcgacgt cttcaaagaa gcacaacacg caggacatgt
     8461 tccgccagga tttaacccag cgcaggcaac aaaacaaccg cgaaatcgag taaaccgcca
     8521 aagattgtca ctgcccgaat ggcaggcaat atttgacagc gtaagcagac ggcagcccta
     8581 tttaaaatgc ggcatgctac ttgctcttgt tactggacaa cgtttaggcg atatctgcaa
     8641 tttgaaattc tctgatatat gggacgacat gttgcacatt actcaggaaa aaaccggttc
     8701 aaaacttgct attccgctta acctgaaatg cgatgctctg aatattaccc ttcgtgaagt
     8761 tatatctcag tgcagggatg ctgttgttag taaatatctg gtccattacc gtcacactac
     8821 ctctcaagca aacagaggag accaggtttc tgcaaatact ctgacaacgg cttttaaaaa
     8881 ggcccgggaa aaatgtggca taaaatggga gcaaggaact gcgcccacat ttcatgagca
     8941 gcgatctctg tcagaacggt tatatcggga acagggtctg gatacgcaaa agttgttagg
     9001 ccataaatcc agaaaaatga ccgaccgata caatgatgat cgtggtaaag actggattat
     9061 cgtagatatc aaaacagcat agaaaatagc cagttttggg gaagggtttt ggggaaagtt
     9121 ttggggaaga ttttacatca tcataaaaca acgggcgtgt tatacgcccg tttcaatatt
     9181 taacacatgt agtgattaca tattcttgat gatcgcgtca ccaaactctg aacatttcag
     9241 cagtttagcg ccttccatca gacgttcgaa gtcataggtt acggtcttgg cattgattgc
     9301 gccttccata cctttaacaa tcaggtcagc cgcttcagtc caacccatat ggcgcagcat
     9361 catctcagcg gagagaataa tagagcctgg gtttactttg tcctgtccgg catatttcgg
     9421 cgcagtaccg tgggtggctt caaacagggc gcattcgtca ccgatgtttg cgccaggggc
     9481 gataccgata ccaccaacct gcgctgccag ggcgtcagaa atgtagtcac cgttcaggtt
     9541 catacaggcg ataacatcat attcagccgg acgcagcagg atctgttgca ggaatgcatc
     9601 agcaatcacg tctttaatga cgatctcttt gccggtattc gggtttttaa ctttcagcca
     9661 cgggccgccg tcgatcagtt caccgccaaa ttcttcacgc gccagttggt agccccagtc
     9721 tttaaacgcg ccttcggtga acttcatgat gttgcctttg tgcaccaggg tcacagagtc
     9781 acgatcgtta gcaattgcgt attcgatcgc tgcacgaacc agacgtttgg tgccttcttc
     9841 cgaacacggc ttaataccga taccgcaatg ttccgggaag cgaattttct tcacgcccat
     9901 ctcttcacgc aggaatttaa tcactttctc agcgtcggca gagtctgctt tccattcgat
     9961 acccgcataa atgtcttccg agttttcacg gaagataacc atatcggtca gttcagggtg
    10021 tttaaccggg cttggagtgc cctgatagta acgtaccgga cgcaggcaga tgtagagatc
    10081 cagttcctgg cgcagggcaa cgttcagaga gcgaataccg ccaccaaccg gagtggtcag
    10141 cgggccttta atggcaacgc gatattcacg aatcagatca agggtttcag caggcaacca
    10201 gacgtcctga ccataaacct gtgtggattt ttcaccggtg taaatttcca tccaggagat
    10261 tttacgctcg cctttatagg ctttctcgac tgcagcgtcg accactttca gcatggctgg
    10321 ggttacatct acaccgattc catcaccttc aatgtaaggg ataatcggat tttcaggaac
    10381 gttgagtttg ccgttttgca gggtgatctt cttgccttgt gccggaacaa ctactttact
    10441 ttccattcac ctctccttcg agcgctactg gtttgctcgt ctgccaccac gttgcatatg
    10501 cgtttgcgtc ctgcgatacg gatgctttag agcaattttt tgttaatgat ttgtaattag
    10561 cttgtcaata ctaccctatt gtttgccgtc atgaaagatg cgcgttatta ggctataatg
    10621 cggcaattca taatctctga aaataccatg caaaaaactt cttttagaaa tcaccaggtt
    10681 aagcgattca gctcgcaacg ttctaccagg cgtaaacctg acaaccagcc cacgcgtgtg
    10741 atcctgttca ataaacccta cgatgttctt ccgcagttca ccgatgaagc cggacgcaaa
    10801 acattaaaag aattcatccc ggttcagggg gtgtatgcgg caggtcgtct cgaccgcgac
    10861 agcgaagggt tactggtgct gaccaataac ggcgcgttgc aggcgcgttt aacccagccg
    10921 ggtaaacgca ccggaaaaat ctattatgtg caggtggaag gtattcccac acaagacgca
    10981 cttgaagcct tgcgcaatgg cgtaacctta aatgatggcc ctaccctgcc cgcaggcgcg
    11041 gagctggttg aagaacccgc gtggttatgg ccgcggaatc caccaattcg tgagcgcaaa
    11101 agtattccca ccagctggct gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc
    11161 cgcatgaccg cccatgttgg tttccccacg ctgcgactga ttcgctatgc gatgggggat
    11221 tactctttgg ataatcttgc caatggcgaa tggcgagacg cgacagatta aggatgcaaa
    11281 atgtttaaac cgcacgttac cgttgcttgc gtggtgcacg cagaaggcaa atttttagtc
    11341 gttgaagaga cgattaatgg taaagcgtta tggaaccaac ctgccgggca cctggaagcc
    11401 gatgaaacct tagtggaagc cgccgcccgt gagctgtggg aagaaaccgg cattagcgcg
    11461 cagccgcaac actttattcg tatgcatcag tggattgcgc ccgataaaac gccgtttttg
    11521 cgtttcctct ttgccattga gcttgagcaa atatgcccga cgcagcctca tgacagcgat
    11581 atcgactgct gccgttgggt cagcgccgaa gaaattttac aggcgtcaaa tcttcgttcg
    11641 ccgctggtgg cggaaagtat tcgttgttat caaagcgggc aacgctatcc gctggagatg
    11701 attggcgatt ttaactggcc ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat
    11761 acgccgcctt gaagttcaat gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa
    11821 tcgtcggcat gtccggcggt gtcgattcct ccgtttctgc ctggctgtta caacaacagg
    11881 gatatcaggt cgaaggcctg tttatgaaga actgggaaga agacgacggt gaggaatatt
    11941 gcacagcggc agcggatctg gctgatgccc aggctgtctg cgacaagctc ggcattgaac
    12001 tgcacaccgt taactttgct gccgagtact gggacaacgt cttcgaactg ttccttgccg
    12061 aatataaagc cggtcgcacg ccgaatccgg atattctgtg caacaaagag atcaaattta
    12121 aagctttcct cgaatttgcc gccgaagatt taggtgccga ttatatcgct accggtcatt
    12181 acgtacgtcg ggcagatgtc gatggcaaga gccgcctgct gcgtggtctg gacagcaata
    12241 aagaccagag ctacttcctt tatacgctca gccatgagca gattgcgcaa agcctgttcc
    12301 cggtcggcga actggaaaaa ccgcaggtgc gtaagattgc tgaagatctt ggtctggtca
    12361 ccgcgaagaa aaaagactct accggcattt gcttcattgg cgaacgtaaa ttccgcgagt
    12421 tcctgggccg ttatctcccg gcgcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa
    12481 ttggtgagca ccaggggctg atgtatcaca ccctcggtca gcgtaaaggt ctgggtatcg
    12541 gtggcaccaa agacggcacc gaagaaccgt ggtatgtggt cgataaagac gtcgaaaaca
    12601 acattctgat tgttgcgcaa ggccatgaac acccgcgtct gatgtctgtc ggtctgattg
    12661 cccagcagct gcactgggtc gatcgcgaac cgttcaccgg tactatgcgt tgcacagtaa
    12721 aaacccgcta tcgccagacc gacatcccgt gcaccgtcaa ggcgctggac gatgatcgca
    12781 ttgaagtgat tttcgatgag ccggttgccg ccgtgacgcc gggccagtct gccgttttct
    12841 ataacggtga agtgtgcctc ggtggcggta ttattgagca gcgtctgccg ctgccggtct
    12901 gattattatc tttacttaaa acaaggaagc agtgaacgtg gcaaagaatt actatgacat
    12961 caccctcgcc ctggccggta tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca
    13021 ggggcattgt gatggcgatg cgctacacgt ctcactcaac agtattatcg atatgaaccc
    13081 cagctcaacg ctggcggttt ttggcggcag cgaagccaac ctgcgcgtcg ggctggaaac
    13141 cttgctcggc gtactcaatg ccagcagtcg ccaaggctta aacgccgaat taacccgcta
    13201 cacactcagt ctgatggtgc ttgagcgcaa actctcctca gcgaaaggtg cgctcgacac
    13261 tctgggcaac cggatcaacg gcctgcaacg ccagctcgaa cacttcgatt tacagtccga
    13321 aacgctgatg agcgcgatgg ctgccatcta tgttgatgtt atcagcccgc ttggcccgcg
    13381 cattcaggtc acgggttccc ctgctgtact gcaaagccca caggtgcagg cgaaagttcg
    13441 cgcaaccctg ctggcaggca ttcgcgccgc cgtgctctgg caccaggtcg gcggcggtcg
    13501 tctgcaactg atgttttctc gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca
    13561 tttaaccccg gagttgtgat ctatggaatt atcctcactg accgccgttt cccctgtcga
    13621 tggacgctac ggcgataaag tcagcgcgct gcgcgggatt ttcagcgaat acggtttgct
    13681 gaaattccgt gtacaagttg aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat
    13741 caaggaagtt cctgcttttg ctgccgacgc aatcggttac cttgatgcaa ttgtcgccag
    13801 tttcagcgaa gaagatgcag cacgcatcaa aaccatcgag cgtaccacta accacgacgt
    13861 taaagcggtt gagtatttcc tgaaagaaaa agtggcggat atcccggaac tgcacgcggt
    13921 ttctgaattc atccattttg cctgtacttc ggaagatatc aataacctct cccacgcatt
    13981 aatgctgaaa accgcgcgtg atgaagtgat cctgccgtac tggcgtcaac tgattgatgg
    14041 cattaaagat ctcgccgttc agtatcgcga tatcccgctg ctgtcccgta cccacggtca
    14101 gccagccacg ccgtcaacca ttggtaaaga gatggctaac gtcgcctacc gtatggagcg
    14161 ccagtaccgc cagcttaacc aggtggagat cctcggcaaa atcaacggcg ctgtcggtaa
    14221 ctacaacgcc cacatcgccg cttacccgga agttgactgg catcagttca gcgaagagtt
    14281 cgtcacctcg ctgggtattc agtggaaccc gtacaccacc cagatcgaac cgcacgacta
    14341 cattgccgaa ctgtttgatt gcgttgcgcg cttcaacacc attctgatcg actttgaccg
    14401 tgacgtctgg ggttatatcg cccttaacca cttcaaacag aaaaccattg ctggtgagat
    14461 tggttcttcc accatgccgc ataaagttaa cccgatcgac ttcgaaaact ccgaagggaa
    14521 cctgggcctt tccaacgcgg tattgcagca cctggcaagc aaactgccag tttcccgctg
    14581 gcagcgtgac ctgaccgact ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc
    14641 gctgattgcg tatcaatcca ccctgaaagg cgtgagcaaa ctggaagtga accatgacca
    14701 tctgctggag gagctggatc acaactggga agtgctggct gagccaatcc agacagttat
    14761 gcgtcgctat ggcatcgaaa aaccgtacga gaagctgaaa gagctgactc gcggtaagcg
    14821 cgttgacgcc gaaggcatga agcaatttat cgacggtctc gcgctgccag aagaagagaa
    14881 agcccgcctg aaagcgatga cgccggctaa ctatattggt cgcgccatca ccatggttga
    14941 tgagctgaaa taagcctcaa atcagtgccg gatggcgatg ctgtccggcc tgcttattaa
    15001 gattatccgc tttttatttt ttcactttac ctccccttcc cgctggttta tttaatgttt
    15061 acccccataa ccacataatc gcgttacact attttaataa ttaagacagg gagaaataaa
    15121 aatgcgcgta ctggttgttg aagacaatgc gttgttacgt caccatctta aagttcagat
    15181 acaggatgct ggtcatcagg tcgatgacgc agaagatgcc aaagaagccg attattatct
    15241 caatgaacat ttaccggata ttgcgattgt cgatctcgga ttgcctgacg aggatggtct
    15301 gtcactgatt cgccgctggc gtagcaatga tgtttcactg ccgattctgg tattaaccgc
    15361 ccgtgagagc tggcaggaca aagtcgaagt attaagtgcc ggtgctgatg attacgtgac
    15421 taaaccgttt catattgaag aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag
    15481 cggtctggct tcacaggtca tttcgctacc accgtttcag gttgatctct cccgccgtga
    15541 attatctatt aatgacgaag tgatcaaact gaccgcgttc gaatacacca ttatggaaac
    15601 gttgatacgc aataatggca aagtggtcag caaagattcg ttaatgctac aactctaccc
    15661 ggatgcggag ctgcgggaaa gccataccat tgatgtactg atgggacgtc tgcgcaaaaa
    15721 aattcaggca caatatccgc aagaagtgat taccaccgtt cgcggccagg gctatctgtt
    15781 cgaattgcgc tgatgaaaaa attactgcgt ctttttttcc cgctctcgct gcgggtacgt
    15841 tttctgttag caacggcagc agtagtattg gtgctttcgc ttgcctacgg aatggttgcg
    15901 ctgatcggtt atagcgtcag tttcgataaa accacgtttc ggctgttacg tggcgagagc
    15961 aatctgttct atacccttgc gaagtgggaa aacaataagt tgcatgtcga gttacccgaa
    16021 aatatcgaca agcaaagccc cacgatgaca ctaatttatg atgagaacgg gcagctttta
    16081 tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc agcccgactg gctaaaatcg
    16141 aacggttttc atgaaattga agcggatgtt aacgatacca gcctcttgct gagtggagat
    16201 cattcgatac agcaacagtt gcaggaagtg cgggaagatg atgacgacgc ggagatgacc
    16261 cactcggtgg cggtaaacgt ctacccggca acatcgcgga tgccaaagtt aaccattgtg
    16321 gtggtggata ccattccggt ggagctaaaa agttcctata tggtctggag ctggtttatc
    16381 tatgtgctct cagccaatct gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg
    16441 agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga acataaccgc
    16501 gaattgctca atccagccac aacgcgggaa ctgactagtc tggtacgaaa cctgaaccga
    16561 ctgttaaaaa gtgaacgcga acgttacgac aaatatcgta cgacgctcac cgacctgacc
    16621 catagtctga aaacgccact ggcggtgctg caaagtacgc tgcgttctct gcgtagtgag
    16681 aagatgagcg tcagtgatgc tgagccggta atgctggagc aaatcagccg catttcacag
    16741 caaattggct actacctgca tcgtgccagt atgcgcggcg ggacattgct tagccgcgag
    16801 ctgcatccgg tcgccccact gctggacaat ctcacctcgg cgctgaacaa agtgtatcaa
    16861 cgcaaagggg tcaatatctc tctcgatatt tcgccagaga tcagctttgt cggtgagcag
    16921 aacgattttg tcgaggtgat gggcaacgtg ctggataatg cctgtaaata ttgcctcgag
    16981 tttgtcgaaa tatctgcaag gcaaacagac gagcatctct atattgtggt cgaggatgat
    17041 ggccccggta ttccattaag caagcgagag gtcattttcg accgtggtca acgggttgat
    17101 actttacgcc ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac cgagcaatat
    17161 gagggtaaaa tcgtcgccgg agagagcatg ctgggcggtg cgcggatgga ggtgattttt
    17221 ggtcgccagc attctgcacc gaaagatgaa taaatatgtc catacttcac gcattacgtt
    17281 aagcatccgt tataatcggt tgcagatacc agcctgtgga tgcttaacat ggaataccaa
    17341 ctcactctta actggcccga ttttcttgaa cgtcactggc agaaacgtcc ggtggtgtta
    17401 aaacgcggct ttaataattt tattgacccg atctctccag acgagttggc gggtttggcg
    17461 atggaaagcg aagtcgacag tcgactggtc agtcatcagg atggcaaatg gcaggtcagc
    17521 cacggtccat tcgaaagcta cgatcatctc ggtgaaacta actggtcatt gttagtgcaa
    17581 gcagtgaacc actggcatga gccgaccgcc gcgctaatgc gaccgttccg tgaactaccg
    17641 gactggcgta ttgatgatct gatgatctct ttttctgtac cgggcggcgg cgtcggcccg
    17701 catctcgatc agtacgacgt gtttatcatt cagggtaccg gtcgtcgtcg ctggcgagtg
    17761 ggcgaaaagc tgcaaatgaa acagcactgc cctcacccgg atctgttaca ggtcgatccg
    17821 ttcgaagcca tcatcgatga agagctggag cctggcgata ttctttatat tccgccagga
    17881 ttcccgcatg aaggctacgc gctggaaaat gcgatgaact attccgtggg ttttcgcgcg
    17941 ccaaatacgc gggaactgat tagcggattt gccgattatg tgctgcaacg tgaactgggc
    18001 ggcaactact acagcgatcc tgatgttcca cctcgcgctc atcctgcgga cgttctgccg
    18061 caagagatgg ataaactgcg tgagatgatg ctcgaattga tcaaccagcc agaacatttt
    18121 aagcaatggt ttggcgagtt tatatcccag tcacgtcatg aactggatat cgcgccgcca
    18181 gagccgcctt atcagccgga tgaaatctac gatgcgctga aacaaggtga tgtgctggtg
    18241 cgcctggggg gtctgcgcgt attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag
    18301 atcgattccc cgcaccgtcc ggcactggat gcgctcgcca gcaacattgc gctgaccgcg
    18361 gagaattttg gcgatgcgct ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc
    18421 aatagcggat actggttctt cgaagggtaa gtttggattt aagccggatg cggcatcaca
    18481 tgccgcatcc cttttcgctt acttccgttg cgccgtcaac tcggcaatac ggacgatcac
    18541 ctgcaccgct ttttccatac cttccagagt cacaaactca tgcttaccat gatagttgta
    18601 accgccagtg aacaggttcg ggcacggtaa tcccataaac gacaactgcg cgccgtcggt
    18661 accgccgcgg atcggtttca gttccggttc aatatcgcag tcacgcatcg cctgctgggc
    18721 gatatcgaga atatgcggat gctcaaccac tttctcgcgc atattgtagt aactgtcttc
    18781 aatcaccagt tcaatgtagc aatcaggatg taaccctttg cccacttttt tggcgatctc
    18841 catcatttta cgtttacgcg cttcaaactg tttacggtcg aaatcacgga tgatgtagtg
    18901 catatcggcc cgttcaacgg tgcctttcat gctcgccagg tgatagaaac cttcatagcc
    18961 ttctgtcatt tccgggcttt catccgccgg aacttccgca tgaatacgtg ccgccagcga
    19021 cagcgcattt accatcactc ctttcgccgt gcccgggtga acattgttac cgacaatttt
    19081 gatattgacc gacgcggcgt tgaagttttc aaactccagt tcacctacgc caccaccatc
    19141 aacggtgtaa gcccagcggg catcgaaggc gtcaacatca aaatgtttcg cccctttgcc
    19201 cacttcttca tccggggtaa aggcgacgcg aatatcacca tgcggaatgt ttttttgttg
    19261 caataccgcc agcgcggtca tgatttctgc aatacctgct ttgtcatcgg cacctaacaa
    19321 ggttttacca tcggtggtaa tcagcgtctg acccagtagc tgatgcagca ccgggaacat
    19381 aaccggtgat aaaacttcat cgccgatacc cagcgcaata tcgccaccgc gatagttttc
    19441 aacaatttgc ggattcacat ttttgccgct gcaatccggt gaggtatcca catgagaaat
    19501 aaagccaatc gccgggatat cgccagggac gttagccggt aacgtcgcca tcaaagtgcc
    19561 cttctcactt aaggtcacat tgataagccc catctcttcg agctgctctt tcagcagatg
    19621 caataacttc cattggcctt ccgtgctggg aacctgtctc acccctgcct ttgattgggt
    19681 atcaagagac acgtagttca aaaatcgctc aagtagttta tccatgtagt caccctcact
    19741 ttttgtgaca acattattaa gaagcaagaa aagacaaata ttgcgtcagg tcacttttag
    19801 ccctgcaagc gggaatattt atcagcatta ccttaatgaa tataaagcta agccagtaat
    19861 tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa ttaccgccct cattaagagt
    19921 caccaaggtg gttaaccaca aaccccgcat cggtaagcca tccgttgcgt ttacatggga
    19981 cagagtaaaa aattgaataa acaaccgagt tcgctttcac cgctggtgca attggcggga
    20041 attcgcaaat gctttgatgg taaagaggtc attccccagc tggatctgac tatcaacaat
    20101 ggcgagttcc tcacgctgct tggcccttct ggctgcggta aaacaaccgt tcttcgcctg
    20161 attgcaggtc tggaaactgt tgattccgga cgcatcatgc tggataacga ggacatcacc
    20221 cacgttccgg cggaaaaccg ctatgtgaac actgttttcc aaagctacgc acttttcccc
    20281 cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca tgcaaaaaac ccccgcagct
    20341 gaaattacgc cccgcgtgat ggaagccctg cggatggtgc agctggaaac cttcgctcaa
    20401 cgcaaaccgc atcagctctc tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg
    20461 gttaacaagc ctcgtctgtt gttgctggat gagtcgctct cagcgctgga ttacaaactg
    20521 cgtaagcaaa tgcagaacga gctgaaagcg ttacagcgta agcttggcat tacattcgtg
    20581 ttcgtaactc acgaccagga agaagcactt accatgtcgg acaggatcgt ggtgatgcgc
    20641 gatggtcgca ttgagcaaga cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg
    20701 tttgttgccg ggtttattgg cgaaatcaat atgtttaacg ccactgtcat cgaacgtctc
    20761 gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat gtaatatcta cgttaacttc
    20821 gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa
    20881 gagattaacg acgacaacca cgccgaaggg ctgattggtt acgttcgcga gcgtaactac
    20941 aaaggcatga cgctggagtc ggttgttgag ctggaaaatg gcaagatggt gatggtcagc
    21001 gaattcttca atgaagacga tcctgacttt gaccactctc tcgaccaaaa aatggccatt
    21061 aattgggtag aaagctggga ggtcgtactg gctgatgaag aacacaagta agttccagaa
    21121 tgtagtgatt gtcactattg tcggttggct tgtgttgttt atctttctgc ccaacctgat
    21181 gatcattggc accagctttt tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac
    21241 gctggataac tacacgcgtc tgctcgatcc gctctatttt gaagtgctat tgcactcgct
    21301 gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg ggctatcctt ttgcctggtt
    21361 tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt
    21421 ctggaccaac tcattgattc gtatttacgg gctgaaaatt ttcctcagca ccaaaggcta
    21481 tctcaacgag tttttgctct ggctgggcgt tatcgataca ccaattcgca tcatgttcac
    21541 gcctagtgcg gtgattatcg gtctggttta cattctgctg ccgtttatgg tgatgccgct
    21601 gtactccagt atcgaaaaac tggataagcc gttgcttgag gcggcgcgcg atctcggtgc
    21661 cagcaagttg cagaccttta ttcgcatcat tatcccactg acgatgccgg gaattattgc
    21721 cggatgtctg ctggtgatgc tgccagcgat gggcctgttc tatgtgtccg acctgatggg
    21781 cggtgcgaaa aacctgctga tcggtaacgt catcaaggta cagttcctca atattcgtga
    21841 ctggccgttt ggtgcagcca ccagcattac gctgactatc gtaatgggcc tgatgttgct
    21901 ggtttactgg cgcgcttctc gtttgctgaa taagaaggtg gaactcgaat gatcggtcga
    21961 ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc aatcattatt
    22021 ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg ttttaccacc
    22081 aaatggtata gcctgctgat gaacaacgac agcctgttac aggcagcgca gcattcactt
    22141 acaatggcgg tgttttcggc gacgtttgct acgcttatcg gttcactgac ggcagttgcg
    22201 ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg gaatgctgtt tgtggtgatg
    22261 atgtcaccag atatcgtaat ggcgatttcg ctgctggtgc tgtttatgct gctgggtatt
    22321 cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc atttgtggta
    22381 gtgacagtgt attcgcgcct gaaaggtttt gacgtgcgaa tgctggaagc ggcgaaagat
    22441 ctcggtgcca gcgaatttac cattctgcga aaaatcattc tgccactggc aatgccagcg
    22501 gtagcggcgg gctgggtgtt aagctttacc ctgtcgatgg acgacgtggt ggtttcttcg
    22561 tttgtcaccg ggccgagtta tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc
    22621 ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc gctggtgatg
    22681 gtaattgcca gccagcttat tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa
    22741 tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg
    22801 ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac gtgccgccag
    22861 gactgcttga acagttcacc aaagaaaccg gtattaaggt tatctattcg acttacgagt
    22921 cgaacgaaac catgtacgcg aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg
    22981 ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag aagatcgaca
    23041 agtcgaagtt aagcaatttc agcaacctcg atccagacat gctcaacaag ccgtttgacc
    23101 cgaataacga ctattccatt ccgtatatct ggggtgcgac ggcgattggc gttaacggtg
    23161 atgcggtgga tccgaaatct gtcaccagct gggccgatct gtggaagcct gagtacaaag
    23221 gcagcctgct gctgaccgac gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg
    23281 gctactccgg taacaccacc gatccgaaag agattgaagc tgcatacaac gagctgaaaa
    23341 agctgatgcc aaacgtcgcg gcgtttaact ccgataaccc ggcgaacccg tatatggaag
    23401 gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc caggcgggta
    23461 cgccaattga tgtggtgtgg ccgaaagaag gcggtatttt ctggatggac agcctggcga
    23521 tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc
    23581 cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac cttgcggcgc
    23641 gtaagctgtt aagtccggaa gtggcgaacg ataaaacgct ctacccggat gctgaaacca
    23701 ttaaaaatgg tgaatggcag aatgacgttg gcgcagccag cagcatttat gaagagtatt
    23761 atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtgatatta ccgcccgttt
    23821 tttatttagc aaggatgtgg ctaatgagcc aggacagtag agttttcttt cgtatattct
    23881 tcggtatcgg tctggtactg atcctcattt ctgttgttat cttttataac cagttcacct
    23941 atagcaaaga cgcgattcat actgaaggcg tcattgttga taccgtctgg cactccagtc
    24001 attctcatcg cacgggtaaa aatggttcgt ggtatcccgt cgttgccttt cgtccgacgc
    24061 cagattacac gctaattttc aattcgagta ttggcagcga tttttatgaa ggcagcgaag
    24121 gcgataaagt taatgtttat tattctccag gacatcctga aaaggctgag ataaataacc
    24181 cgtgggttaa tttttttaaa tgggggttta tcggcattat gggcgttatt tttatctccg
    24241 tcggtttatt aatcagtatg ccctcctcta aaaagacacg caggaaacgg aagtcccgcc
    24301 catgaaaaaa atcataatat tgctcagttt actgatactc ctgcctctga cggcaaccag
    24361 taagccctta attccaataa tgaagacact ttttacggat gttaccggta ctgtaccaga
    24421 tgctgaagag atcgcgcata aagccgagct ttttcgccaa caaaccggag ttgcaccgtt
    24481 tattgtggtt ttacccgaca ttaataatga agccagtctc aggcaaaatg gcaaagcgat
    24541 gctggcgcat gcggcatctt caatgagtaa tgtaaaagga agtgttctgt tactctttac
    24601 tacccgcgag ccacggttaa ttatgataac caacggccag gttgaaagcg gtctggacga
    24661 taaacatctc ggccttctgg tagaaaatca cacgctggct tatttacatg cggacctctg
    24721 gtatcagggt atcaataatg cattggctgt tttacaagca cagatattaa aacaaccgac
    24781 gccaccactg acatattatc cgcatcccgg acaacagcat gaaaatgatc cacccggcag
    24841 taccacgaca ttaggccttt ttgcctgggc agttgcattc atcgtctttg ctgcattttt
    24901 taactacaca acgcgccttt attatgcgtt aaaatttgca gtggcgatgg ctgttgccaa
    24961 tatgggttat caggctttat gtttgtatat cgataacagc tttgcgatta ccagaatatc
    25021 gccactctgg gcagggctga ttggtgtctg tacgtttatt gccgcgttac tatggacgag
    25081 taaacggtaa aagaggcagt aaagttcgcg tgtattatgc cggatgcggc gcgaacgcct
    25141 tatccggccc acaaaacccg caaattcaat taattgcgcc ccttcacagg cctgacaagc
    25201 gtagcgcatc aggcaatttt tcccgcttta taatcccttc agcaactttt caacaaactc
    25261 tggcaccacc tggcttgccg gaccgtaata tttctcggca aattcattac caacctgact
    25321 cggttcaaga ttcagttcca cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc
    25381 agccgccgga taaacatgcc ccgaagtacc aatggcaatg aaaatatcgg ccatcgacaa
    25441 cgccatataa atttcatcca tgccgagtgg catttcgcca aaccacacta cgtgcgggcg
    25501 caagggtgcc gggaactggc aacaatggca tttatcttct ggcgtaacgt ctccggtcca
    25561 gtcgaggacc tggccacttt gcgaacaacg tactttcagc aattccccat gcatatgaat
    25621 cacattggta ttgcctgcgc gttcatgcag gttatcgata ttttgcgtca ccagcaaaaa
    25681 gcgatcgccg agagcatctt gcagtttcgc cagcgcaaga tgtgcagcgt taggctgaat
    25741 ttctggctgc tgcaactgtc gacgacgggc gttataaaac gtttgcacca gttcaggatc
    25801 gcgatcaaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc acaggccatc
    25861 tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc cctgcccctg tcagtacgag
    25921 tactcttggt ttttccattg cttccggcac cactttatct ctgaaaaaaa tacgctgacg
    25981 caaacgctcg cgcaggcggc gtttattttt acgaaaacga cttaaccgat gaccccgacg
    26041 cgacagcata gcaacctctg ttgattaatc ggttagatgt aggaaggccg caccacgcat
    26101 tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga acacgagcta caggtaagag
    26161 atgacgaggc aacctttccg ccagttgcgt tgtgattgcc gggaaattcg ataagccacc
    26221 accaatgacg accaggtcag ggtcaacaat ggtcaggata tttcccagac aaaccgctaa
    26281 taaatccaga taacgctcaa cgtgcgccct tgcctgctca tcgccttgat cataaagcgc
    26341 aatgatctca ggagcctgca acggttgatg ataatagtgt tgatacagcc acgcaaaacc
    26401 gcgaccagac agataatttt caatgcagcc atgctgacca cagccgcagc ggcgtaacgg
    26461 gaaatccagc cccatcatgg ttaacgcatc aaccggcaga cgcatatggc caaactcgcc
    26521 ggtaatatag cttttaccgg taattggttt accgttgaaa atcagcccgc cgccaacgcc
    26581 ggtgccgaga atcaacccca tcaccagtgg atattgagta aattcatcat cccatgcttc
    26641 agaaagggca aaacagttgg catcgttatc aaggcgtaca tcgcgatcaa gacgtgcgct
    26701 caggtcggca cgcagcggtt taccgctggc agcagggaca ttggcggcat acagcgtacc
    26761 atcttctgtt tccggcatac ccggaatacc aatgccgaca gaacctttac agccaaaacg
    26821 ttggtcagct tcggctacca gctcgcacac tgcatctaaa aatgcgtcat agctgtcacg
    26881 cggtgtcggc acccgctttt cccactgcaa ctgccgaccg ctatcaaaca cgcctagcgc
    26941 aatttttgtt ccaccaatat caaacccgta atacattgcc gctccttgtt ttaatgtact
    27001 gcctttactg gccgctaagg actcgcgcag ggtcaatatt actggcgcgc cgcgccggat
    27061 accaacttgc caaaagactc agcaacaatg ctgtgaccag tacgtagaag acgtccagcc
    27121 agtgcaattc cgatggcagg aagtcaataa aatagatatc gctggagagg aactgatggc
    27181 cgatcagctt ttcaatccac tcaataatcg gggtaagttg cagtgaaaca actacgccga
    27241 taatcacacc acacaggctg ccgaacagcc ctgccagcaa tccataccag acaaagatgg
    27301 cgcggattaa accatctttc gcacccaacg ttcttaatac tgcgatatcg ccactcttgt
    27361 ctttcaccgc catcactaag gtggagacga tgttgaaaca ggccacacca atcaccagta
    27421 ccatcgccag atacataatg gcgcggatca tctgaatatc gcgatacata tagccgtaag
    27481 taccaatcca gcttttaata taaacatagc tgttggtcac ttcacccgca tcgcgtacca
    27541 gcttattggc gttgaaaaca tccgtcattt taagggcaat acctgacacg ctggaaccca
    27601 tatcaagata ttgctgagca tcggccagcg ggatcatggc aaaactgtga tcgagttgac
    27661 cactcaactg caaaataccg gcaacgtgca aacgcacacg ttttggctgc atcagtttat
    27721 gctcgggatt cgaatttggg atcataatcg acacccaatc cccctgcttc actttcagcg
    27781 catccgccac gcctttgccg atgataattt gctgttcgcc tgctttaaaa ttgctccacg
    27841 catcgtcctg gacaaacgag ggtaatgcgc tcaaacgctg ttcctgttgc gggttaacgc
    27901 ccttgacctg gattgcgcgc agattcgcgc cactttccac cagcccggtg aaattgatat
    27961 acggcgcagc ggcggcaatg cccggcactt tctgcacgtt atccagtgct tcctgccagt
    28021 tggtccacgg ctgatctacc gcctcaattt caccgtgcgg tactaccgcc agaatgcggt
    28081 tattcagttc gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat accgccacgc
    28141 cgagggcaat gccaatggta gaaatgacgg agatcaacga caccatgccg ccgcgtcgcc
    28201 gtccgcggct aaaacgcagg ccaatcaata acgataaagg catcgccatt actccgcccc
    28261 catcaggctc agttccgccg tcagacgtcc atcacgcatt tctagttggc ggctcatacg
    28321 tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg gtgccctgca agcgattcaa
    28381 ttccccaagc aactggaaga tgctgtcggc gttacgtgca tcgaggttac cagtaggttc
    28441 atccgccagt accagccgcg ggttattgac cagcgcacgg gcaatcgcca cacgctggcg
    28501 ttcgccgcca gaaagttcag atgggcggtg attcgcacga tgctccagcc ccaccgcttt
    28561 taacatctcc agcgcacggc tgttgatttc agcgggcttt ttcttgccaa tcaacagcgg
    28621 catagccacg ttttccaggg cggtaaaatc cggcagcagg tggtgaaact gataaataaa
    28681 tcccagcttc tggttgcgca gttctgcttt cgccgccgaa gacagtttgc tcatcggttg
    28741 accgttaaag atcacatcgc cggaggttgg cgtatccagc ccgcctaaca ggtgcagcaa
    28801 ggtgctttta ccggaaccag agctgccgac gatcgccatc atttcacctt cgcccacgct
    28861 gaaactgaca ttgtgcagta catcggtttg cacactgcct tcctgatagc gtttgcacag
    28921 gttgtcgcat tgcaacagga tcttattcat aacgtaaagc ctcagcgggt tgagtggcgg
    28981 cagcgcgcca ggaagggtaa agcgtagaca gcagcgcgat agccatcgcc accagcgcaa
    29041 taacaatgac ctgtaaaggc tcgatagcga ccggcagcgc cgcgccatca agcaggacgc
    29101 cgattatcgg catcagatta ttcaactggc tggcaagcag tgcgccaagc gccgctccga
    29161 ggatcgcacc gataatcccg gcgctggccc cttgcaccat aaagaccatc atgatttgcc
    29221 gcggagtcag cccctgagtc tgaagaatgg ccacttcgcc ctgcttctcc atcaccatca
    29281 gtcccagcga ggtaataatg ttaaacgccg caacggcgac aatcaggctc agcagcaagc
    29341 ccatcatatt tttttccatg cgtacggcct ggaacagctc gcctttacgg tcacgccagt
    29401 cctgccattt gctgccttca ggcagttttt gctgacttaa tgagtcgact ttcagcggct
    29461 catccagcca caaacgccag ccggtaatat tgcctgccgg ataacgcatc agacgcgagg
    29521 catcctcaat attcaccagc atttcatagc catcgacttc actgttggcg gcgaaagtgc
    29581 caatcacatt gaacaggcgc tgacttggaa tacgccccat cggcgtgaac tggctagcag
    29641 atggcaccat cacgcggatt tgatcaccgc gattgacgcc caactgtgag gcaagctgtt
    29701 caccgaggat gacattatat ttccccggct cgaggtcagt ttgtttcaca ttgaccagat
    29761 acggcgttag tggatctttt tgcgccgggt cgatacccag catcaccccg accgccacgc
    29821 tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt
    29881 ccagtttgac tgccgtttcc gggagttgct gcgggttaag agagccatgc tcagaagaga
    29941 gaattgcctg tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca aagccgttca
    30001 tcactgacaa tactgtgacc agcgccatca ccccgagggt aatgccgatg gtagaaagcc
    30061 aggagacgaa acgaccgaag cgatccgctg cacgcccacg catgtaacgc aggccaataa
    30121 atagagcgac aggttggtac atgaaatccg tctggttgct gtagcaaaga cacgagtata
    30181 taagcgaaag caggatgggt aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa
    30241 aagtaccagc aaatcaatcg gctaaagtgc ctcattttat catcggccat gtggcctgat
    30301 ctttatctga aaaataacca aatacagaca gatacgcatt acaccacgcg actcgccccg
    30361 caggatcaac cgcatgttaa ataaaaagag cgtgttgtcc cgatgaaaca aaaagagcta
    30421 tggattaacc agatcaaagg gttatgtatt tgtctggtgg tgatttatca ctcggtcatt
    30481 accttttatc cgcatctgac cacttttcag catccgttat cggaagtcct gagcaaatgc
    30541 tggatctatt tcaatcttta ccttgcaccc tttcgtatgc cggttttttt ctttatctct
    30601 ggctatttga ttcgccgcta tatcgacagc gtaccgtggg gaaattgtct cgataaacgc
    30661 atctggaaca tcttctgggt gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt
    30721 gcgctgaacc agtggctggc acctgagcgc gatttaagca atgcctccaa tgccgcttat
    30781 gccgattcca ccggtgagtt cctgcacggg atgatcaccg ccagcaccag cttgtggtat
    30841 ctgtatgcgt taattgtcta tttcgtgata tgtaaaattt ttaaccccct ggccctgccg
    30901 ttatttgttc tgtttgtact gatgagtgtg gctgttaatt tcgtacccac accgtggtgg
    30961 ggaatgaaca gtgtgatccg caatttgcct tattacagcc ttggcgcatg gtttggcgca
    31021 acattaatga cctgtgttaa agcggtaccg ttgcgccgcc atctgctgat ggcttctttg
    31081 ctgaccgttc tggcggtcgg tgcctggttg tttaatatct cgctgctgtt gtcgctggta
    31141 tcgattgtgg taatcatgaa gctgttttat cagtacgagc agcgtttcgg tatgcgttcc
    31201 tccagcctgc tgaatgtgat tggctccaac accattgcta tctataccac ccatcgcatt
    31261 ctggtagaaa tattcagctt aagcctgatt ccgcaaatga acgcggtacg ctggtcgccg
    31321 caagtcgaac tgacgctcct gctgatttac ccctttgtca gtttgttgat ttgtactgtt
    31381 gcgggcttgc tggtaagaaa actttcacag cgcgcattca gcgatctgtt gttctccccg
    31441 ccttctctgc ccgctaccgt cagttactcc cgctaagcca tcgccccagc cggggcgatt
    31501 caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca gagatatgcc
    31561 ccatatgttg aggcatatcc taacgagaat ctgacaaccg ttatgcctga acaatatcgt
    31621 tatacgctgc ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac cggcgctgcc
    31681 tgcgcgacgc tggtagcaga aattgccgaa cgtcacgccg gtccggtggt actcattgca
    31741 ccagatatgc aaaatgctct gcgtttgcat gatgaaatca gccagttcac cgatcagatg
    31801 gtgatgaatc tggcggactg ggaaaccctc ccttacgaca gtttttcgcc tcatcaggac
    31861 attatctcct cgcgtctttc caccctttac cagttaccat cgatgcagcg tggcgtactg
    31921 attgttccgg tgaatacgct tatgcagcgc gtttgcccgc acagctttct ccacggtcat
    31981 gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac ccaactggac
    32041 agcgccggtt atcgccatgt tgaccaggtg atggagcacg gcgaatacgc cacgcgcggc
    32101 gcgttgttgg atctcttccc gatggggagt gagctgcctt atcgtcttga tttctttgat
    32161 gatgaaatcg acagcctgcg ggtgtttgac gtcgatagcc agcgcacgct ggaggaagta
    32221 gaagcgatca atctgttgcc cgcgcacgaa tttccgaccg ataaagcggc aattgaactg
    32281 ttccgcagcc agtggcgtga taccttcgaa gtgaagcgcg acccggaaca tatttatcag
    32341 caagtgagta aaggcacatt acctgccggg atcgagtact ggcagccgct attcttcagc
    32401 gaaccgctgc cgccactgtt cagttatttc cctgccaata cgctgttggt gaatactggc
    32461 gatctggaaa acagtgccga acgtttccag gctgacacgc tcgcgcgttt tgagaatcgc
    32521 ggcatcgacc caatgcgccc actgttgcca ccacaatcgc tctggctgcg ggtggatgag
    32581 ctcttctcag agctgaaaaa ctggccccgt gtgcagctaa aaactgaaca tttaccgaca
    32641 aaagcagcga atgccaattt aggtttccag aaactgccag acctggccat tcaggcacaa
    32701 caaaaagcgc cgctggatgc gctgcgtaag ttcctcgagt ctttcgacgg tccggtggtg
    32761 ttctcggtag aaagtgaagg ccgccgtgaa gcgttgggtg aactgctcgc gcgaattaaa
    32821 attgctccgc aacgcattat gcgtcttgat gaagccagcg accgtgggcg ttatctgatg
    32881 attggcgctg cagagcatgg ttttgtcgat acggtacgta atctggcgct gatttgcgaa
    32941 agcgatctgc tcggtgaacg cgttgcccgt cgtcgtcagg attctcgccg cgccatcaac
    33001 cccgatacac tgatccgtaa ccttgcggag ctgcatattg gtcagccggt ggtccatctg
    33061 gagcacggcg tcggtcgtta tgccggaatg accacgctcg aagctggagg cattactggc
    33121 gagtatttga tgctcaccta tgccaacgac gccaaactgt atgttccggt gtcgtcactg
    33181 catctgatta gccgttacgc gggtggcgca gaagaaaacg caccactgca taaacttggt
    33241 ggcgatgcct ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga tgtggcggcg
    33301 gaattgctgg atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt taaacacgat
    33361 cgcgagcagt atcagttgtt ctgcgacagc ttcccgtttg aaaccacgcc ggatcaggcg
    33421 caggccatta atgcggtgct tagtgacatg tgtcagccgc tggcaatgga tcgtctggtg
    33481 tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt catggcggta
    33541 gataaccaca aacaggtggc ggtgctggtg cctaccaccc ttctcgcgca gcagcattac
    33601 gacaacttcc gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat ctcccgtttc
    33661 cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg cggaagggaa aattgatatt
    33721 ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt aggcctgctg
    33781 attgtcgatg aagaacaccg cttcggggtg cgtcataaag agcgcattaa agcgatgcgc
    33841 gcgaacgtgg atattctgac gcttaccgca acgccgatcc cacgtacgct gaatatggca
    33901 atgagcggaa tgcgcgatct gtcgattatc gccacgccgc ccgcccgtcg tctggcagtt
    33961 aaaacctttg tccgtgagta tgacagtctg gtggtccggg aggcgatcct gcgtgaaatt
    34021 ttgcgcggag gacaggttta ttatctctac aatgatgtgg aaaacatcca gaaagccgcc
    34081 gaacggctgg cagaactggt acctgaagcg cggattgcta tcggtcacgg gcaaatgcgt
    34141 gagcgcgaac tggaacgggt gatgaatgat ttccatcatc aacgtttcaa cgtgctggtt
    34201 tgtaccacca ttatcgaaac cgggatcgac attcctaccg ccaacaccat tatcattgaa
    34261 cgcgcggatc acttcggtct ggcacagttg caccagttgc gcggtcgcgt cgggcgttcg
    34321 caccaccagg catatgcatg gctgctgacg ccgcatccaa aagcgatgac taccgatgcg
    34381 caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg gtgcaggttt tgcgctggca
    34441 acgcacgatc tggagatccg cggcgcgggt gaactgcttg gcgaagaaca aagcggctca
    34501 atggaaacca tcggtttctc gctgtatatg gagttgctgg aaaacgccgt cgatgcgctg
    34561 aaagccggac gcgagccgtc gctggaagat ctcaccagcc agcaaacaga agtcgagctg
    34621 cggatgccgt cgctattgcc agatgatttc atccctgacg tgaacacgcg tctgtcgttc
    34681 tacaaacgta ttgccagcgc caaaacggaa aacgaactgg aagagatcaa agtcgagctt
    34741 atcgatcgct tcggcctgct gccggatccg gcgcgtaccc tgctggatat tgcccgtctg
    34801 cgccagcaag cgcagaaact ggggatcagg aagctggaag gtaatgagaa aggcggcgtg
    34861 atcgaatttg ccgagaagaa tcacgttaat ccggcctggt tgattggttt gctgcaaaaa
    34921 cagccgcagc attaccgcct tgatggtccg acgcgtctga agtttattca ggatttgagc
    34981 gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc gtgaactgga agagaacgcg
    35041 atcgcttaat ggtaaatcgg gggcgtttct gcgcccccat ctgcaatatt tacaaattct
    35101 ttgcacttcc ctgcattatc cgacactgtc accctccata attcagactt acctttttat
    35161 tacaataacc ttatatttat tatggacttt ggtgatgatc aaaacgcgtt tttctcgctg
    35221 gctaacattt tttacgttcg ccgctgccgt ggcgctggcg ctaccggcaa aagccaacac
    35281 ctggccgctg ccgtcagcag gcagtcgtct ggttggtgaa aacaaatttc atgttgtgga
    35341 aaatgacggt ggttctctgg aagccatcgc caaaaaatat aacgtcggct ttctcgctct
    35401 gttacaggct aaccccggcg ttgatcctta cgtaccgcgc gcgggtagcg tgttaacgat
    35461 cccgttgcaa accctacttc cagacgcgcc gcgcgaaggc attgtgatca acattgcgga
    35521 gctgcgtctc tattattatc cgccgggaaa aaattcggta accgtatatc ccatcggcat
    35581 tggtcaatta ggtggtgaca cgctgacacc gacaatggtg accaccgttt ctgacaagcg
    35641 tgcaaaccca acctggacgc caacggcaaa cattcgcgcg cggtataagg cacagggaat
    35701 tgagttgcct gcggtagttc cggctggacc ggataaccca atgggccatc atgcgattcg
    35761 tctggcggcc tatggcggcg tttatttgct tcacggtacg aacgctgatt tcggcattgg
    35821 catgcgggta agttctggct gtattcgtct gcgggatgac gatatcaaaa cactctttag
    35881 ccaggtcacc ccaggcacca aagtgaatat catcaacacc ccgattaaag tctctgccga
    35941 accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg tcagagaaga ttgatgacga
    36001 tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa tcatttaaag atgcagcaca
    36061 aactgacgct gaagtgatgc aacatgtgat ggatgtccgt tccgggatgc cggtggatgt
    36121 ccgccgtcat caagtgagcc cacaaactct gtaaagctcg gataaaaaaa tccccgcgac
    36181 acggcatgtc gcggggaaaa aaacaagcag tagcaggcat taatgagggt taatgcttat
    36241 ttataaatta ctgctgttcc atggagggta ttcggaccgg ttacagaagt aatacggaaa
    36301 gattttgcgc ccatctcatc cgctttttgc gccagctgct cttccagcga tcccagattt
    36361 gtccccgcgt tagcactgat agtaccgact ttttgttggc cttctggcgt tgactgaact
    36421 tcgacagccg caaagctggc aaatgacatg gagcttaaaa tcgccgcagc gatgagggtt
    36481 tttacgtttt tcataatagt ggccttatgc agatgaatga cgtcgcaagc aattacttaa
    36541 cgatcgataa ctaaatgata ga
//
LOCUS       DACADA010000031        36219 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.031, whole genome shotgun sequence.
ACCESSION   DACADA010000031 DACADA010000000
VERSION     DACADA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.031"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(123..410)
                     /locus_tag="ISB17_001536"
     CDS             complement(123..410)
                     /locus_tag="ISB17_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979480.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ISB17_001537"
     CDS             complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ISB17_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAP1979481.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            787..1026
                     /locus_tag="ISB17_001538"
     CDS             787..1026
                     /locus_tag="ISB17_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979482.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1170..1961)
                     /gene="kdgR"
                     /locus_tag="ISB17_001539"
     CDS             complement(1170..1961)
                     /gene="kdgR"
                     /locus_tag="ISB17_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAP1979483.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2138..3511
                     /locus_tag="ISB17_001540"
     CDS             2138..3511
                     /locus_tag="ISB17_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979484.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3557..4438)
                     /gene="htpX"
                     /locus_tag="ISB17_001541"
     CDS             complement(3557..4438)
                     /gene="htpX"
                     /locus_tag="ISB17_001541"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAP1979485.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4630..6678)
                     /gene="prc"
                     /locus_tag="ISB17_001542"
     CDS             complement(4630..6678)
                     /gene="prc"
                     /locus_tag="ISB17_001542"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAP1979486.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6698..7396)
                     /gene="proQ"
                     /locus_tag="ISB17_001543"
     CDS             complement(6698..7396)
                     /gene="proQ"
                     /locus_tag="ISB17_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAP1979487.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7493..7990)
                     /locus_tag="ISB17_001544"
     CDS             complement(7493..7990)
                     /locus_tag="ISB17_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP1979488.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8120..9403
                     /gene="yebS"
                     /locus_tag="ISB17_001545"
     CDS             8120..9403
                     /gene="yebS"
                     /locus_tag="ISB17_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAP1979489.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9372..12005
                     /gene="yebT"
                     /locus_tag="ISB17_001546"
     CDS             9372..12005
                     /gene="yebT"
                     /locus_tag="ISB17_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAP1979490.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12085..13524
                     /gene="rsmF"
                     /locus_tag="ISB17_001547"
     CDS             12085..13524
                     /gene="rsmF"
                     /locus_tag="ISB17_001547"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAP1979491.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13641..13877
                     /locus_tag="ISB17_001548"
     CDS             13641..13877
                     /locus_tag="ISB17_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAP1979492.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13898..14173
                     /locus_tag="ISB17_001549"
     CDS             13898..14173
                     /locus_tag="ISB17_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAP1979493.1"
                     /translation="MAGYLSGLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(14174..14830)
                     /gene="pphA"
                     /locus_tag="ISB17_001550"
     CDS             complement(14174..14830)
                     /gene="pphA"
                     /locus_tag="ISB17_001550"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP1979494.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15225..15566)
                     /locus_tag="ISB17_001551"
     CDS             complement(15225..15566)
                     /locus_tag="ISB17_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAP1979495.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15579..16451)
                     /gene="copD"
                     /locus_tag="ISB17_001552"
     CDS             complement(15579..16451)
                     /gene="copD"
                     /locus_tag="ISB17_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAP1979496.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16455..16829)
                     /gene="yobA"
                     /locus_tag="ISB17_001553"
     CDS             complement(16455..16829)
                     /gene="yobA"
                     /locus_tag="ISB17_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAP1979497.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16968..17198
                     /locus_tag="ISB17_001554"
     CDS             16968..17198
                     /locus_tag="ISB17_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1979498.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17300..17956
                     /locus_tag="ISB17_001555"
     CDS             17300..17956
                     /locus_tag="ISB17_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAP1979499.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17980..18642
                     /gene="exoX"
                     /locus_tag="ISB17_001556"
     CDS             17980..18642
                     /gene="exoX"
                     /locus_tag="ISB17_001556"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAP1979500.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ISB17_001557"
     CDS             complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ISB17_001557"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAP1979501.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20908..21567)
                     /locus_tag="ISB17_001558"
     CDS             complement(20908..21567)
                     /locus_tag="ISB17_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAP1979502.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21894..22250)
                     /gene="yebF"
                     /locus_tag="ISB17_001559"
     CDS             complement(21894..22250)
                     /gene="yebF"
                     /locus_tag="ISB17_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAP1979503.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22317..22607)
                     /gene="yebG"
                     /locus_tag="ISB17_001560"
     CDS             complement(22317..22607)
                     /gene="yebG"
                     /locus_tag="ISB17_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAP1979504.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22741..23919
                     /gene="purT"
                     /locus_tag="ISB17_001561"
     CDS             22741..23919
                     /gene="purT"
                     /locus_tag="ISB17_001561"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAP1979505.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23975..24616)
                     /locus_tag="ISB17_001562"
     CDS             complement(23975..24616)
                     /locus_tag="ISB17_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP1979506.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24653..26464)
                     /gene="edd"
                     /locus_tag="ISB17_001563"
     CDS             complement(24653..26464)
                     /gene="edd"
                     /locus_tag="ISB17_001563"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAP1979507.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26699..28174)
                     /gene="zwf"
                     /locus_tag="ISB17_001564"
     CDS             complement(26699..28174)
                     /gene="zwf"
                     /locus_tag="ISB17_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP1979508.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28512..29381
                     /locus_tag="ISB17_001565"
     CDS             28512..29381
                     /locus_tag="ISB17_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1979509.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29509..30951
                     /gene="pyk"
                     /locus_tag="ISB17_001566"
     CDS             29509..30951
                     /gene="pyk"
                     /locus_tag="ISB17_001566"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP1979510.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31083..32054)
                     /gene="lpxM"
                     /locus_tag="ISB17_001567"
                     /gene_synonym="msbB"
     CDS             complement(31083..32054)
                     /gene="lpxM"
                     /locus_tag="ISB17_001567"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAP1979511.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ISB17_001568"
     CDS             complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ISB17_001568"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAP1979512.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ISB17_001569"
     CDS             complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ISB17_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAP1979513.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34522..35277
                     /gene="znuC"
                     /locus_tag="ISB17_001570"
     CDS             34522..35277
                     /gene="znuC"
                     /locus_tag="ISB17_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAP1979514.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35274..36059
                     /gene="znuB"
                     /locus_tag="ISB17_001571"
     CDS             35274..36059
                     /gene="znuB"
                     /locus_tag="ISB17_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAP1979515.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
BASE COUNT         8665 a         9292 c         8923 g         9339 t
ORIGIN      
        1 atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagttgcac catgacagtg
       61 cgtataatca gcatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag
      121 gattatcgtt cggcgaccag acgaataata tcttcgtctt caaccgattt ttgactctca
      181 acggtctttt tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg taacagtgca
      241 ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccgt
      301 gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac caccaccgat
      361 acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct
      421 ctgagtagag gcgctattct accactgctg gagaggaaga aaatccagtg ctgaaaaaat
      481 gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct
      541 gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa
      601 ccagagcgac ccatcgaaac tttttcactc caatctccgt ttcacctacc ttatgtcatt
      661 aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc tttttcagaa
      721 tgagtcgctt gcttcagcgc atattgccgc tacgattaag cgaacataaa aagagaagag
      781 gttgtaatgc gattcatcat tcgcacagtt atgctgatag cactggtatg gataggatta
      841 ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcggg attaggtttg
      901 caatgtacct atctgaccgc cagaggcacc agcacggtac aatacctgca cactaaaagt
      961 ggttttctgg ggataacaga ttgcccgttg ttacgcaaaa gcaatattgt ggtcgataat
     1021 ggttaagggt aataaataac cagattctca ctcatgagta aaatattctt atcgcaagtg
     1081 cgtttaatat aacgccacct tcaactttat ttttctcatg ctaaataaag aacctgtctg
     1141 tttgatattc agacaggttc ttactctcat cagaacggat agtcgtgata acccatttgg
     1201 gcagaaattt tgcgcgccgc ggtatgcaac attgcgacat attcctgtaa acgctcttca
     1261 gagaaacgca acgtcgggaa ggagatgctc agaccggcaa tgaccacgcc aaagcgatca
     1321 aataccggaa ccgcaatgca acgcagtcct tcttcctgct cttcgttatc ttcgccatac
     1381 ccctgctcgc gcacctggtc cagaacgggt aataacgctt ctgtactgat gatggtccgt
     1441 tcggtactgc gtttatactc cacgccctca agaatttgca tcacttcatc gcgatcgcgc
     1501 catgccagca gtaccttacc aatcgcggtg ctgtacagcg gattacgacg cccaatccgt
     1561 gaatacatgc gcaaattgta cattgagtca attttgtgga tgtaaacaat actgtcttcg
     1621 tccagtgcgc cgaggtggat agtttctttg gtcaggcggg agatctcacg catctggata
     1681 tctgcgctac gaattaaatc gacgttttgc aacgcgcggg cgcccagttc aaacaatttc
     1741 agggtcagcg aatatttctc cgactcccct tcctgcgcca cataacctaa ggttttcatg
     1801 gtctgtaaaa agcgataaac ggtgcttttt gacatcatga cgcgctgcga caactcggtt
     1861 atccctattt cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa
     1921 gatacagaat caggctgttt atccagatct gcgttagcca tttatcacct cattgcgagt
     1981 gttttataaa aatcagaact gttttttatt ataatttcgc acctgggtgg tcgcaatcca
     2041 tcttttgccg gttagttaca attctgcgac atccaccgtg aatatcagtg ctagaatcat
     2101 acccctgttg attattcacc aaagatataa aattcctatg ccaaaagttc aggccgacgg
     2161 cctgccattg cccctgcgat acggtgcgat attaaccatt gtgattggta tttcgatggc
     2221 tgtccttgac ggcgcaatcg ccaacgtcgc cttgccaaca atcgctacgg accttcatgc
     2281 cacgccggcc agttccatct gggtagtgaa cgcctatcaa atcgccattg tcatctccct
     2341 gctctcgttt tcgtttctgg gcgatatgtt tggctatcga cgtatttata aatgcggtct
     2401 ggtcgttttt ctgttgtctt cactgttctg cgccctttct gattcgctgc aaatgctcac
     2461 ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta ataccgcact
     2521 tatccgcctg atctatccac aacgttttct gggtagaggg atgggcataa actcgtttat
     2581 tgttgccgtc tcttctgctg ccgggccgac aattgctgca gcaatcctct ccatcgcatc
     2641 ctggaaatgg ttatttttaa tcaacgtacc gttgggtatt atcgccctgc ttctggcgat
     2701 gcgttttctg ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc
     2761 cgtgatgaac gcgttaacct tcggcctgct tattactgca ttgagtggtt tcgctcaggg
     2821 gcaatcgctg acattgattg gtgcggaact ggtggtaatg gttgtcgttg gtattttctt
     2881 tattcgccgc cagctttctc ttcccgtacc gctgctaccg gtggatttac tgcgtatccc
     2941 gctgttttca ctttctattt gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat
     3001 ggtttccctt cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt
     3061 acttctgaca ccgtggccgt tagcaacgat ggttatggct ccactggcag gctatttgat
     3121 tgaacgcgta catgcaggat tgctgggtgc tttagggtta ttcatcatgg ctgcggggct
     3181 tttttccctg gttctgctgc cagcgtcacc tgcggatatc aatattatct ggccgatgat
     3241 cttatgtggt gctggatttg gcttgttcca gtcacccaat aaccacacca ttattacctc
     3301 cgctcctcgc gaacgtagcg gtggagccag tggcatgtta gggacggctc gtcttctggg
     3361 tcagagtagc ggcgcggctc tggtagcgct gatgctaaat cagtttggtg ataatggtac
     3421 gcacgtctcg ctgatagctg cggctattct ggcagtgatt gctgcctgtg tcagtggttt
     3481 acgtatcact cagccacgat ccagggcata aaaaaagcgc gtcgatcagg acgcgctttt
     3541 tagtatttac gtcatattac ttcaggtatt cacccgtacg cagagcttca atacgtttat
     3601 ccagcggcgg gtgggtcatg aacaactcac tgagcgattt cgacttaccg ttaatgcaga
     3661 aagccatcat gctggttgct tcttgcggtt catagctggt tttcaggcgt tgcagcgcgg
     3721 caatcatttt ctcgcgacca accagttttg ctgaacccgc atcagcatgg aattcacgat
     3781 gacgcgagaa ccacatggtg ataatgctcg ccagaatacc aaacaccagt tccagaaccg
     3841 ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc ttcaccttca tcacggtttc
     3901 cgcccataaa acccgcggca agctgcgcca gaatacggga aataaagata acgaaggtgt
     3961 tcaccacgcc ctgaatcagc gtcatggtga ccatatcacc attggcgatg tggctgattt
     4021 cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt ctgcagcaaa ccagtgctga
     4081 cagcaaccag agaggcatca cggcgcgcac cagttgcaaa agcattgatg tccggcgcat
     4141 ggtagatagc cacttgcggc atagcgatcc ccgcctgacg agcctgggtt gctacagtat
     4201 tgaccagcca acgttccctt tcgttacgcg gttgctcgat cacttccccg ccaacagatc
     4261 gtaatgccat ccatttggac atcagaagcg aaacgaagga accaccaaaa ccgaacagca
     4321 aggccatgat catcagcccc tgaacgctgc tcgactgtat ccctgtcagg ctcagtacca
     4381 gcccgaaaac gaccattacg gccaggttcg ttagcaggaa gagcgcgatt cgcatcataa
     4441 ttttcttttt acctcagttt aacaaaacgc aatatgcgat acccacatcg tatgggttac
     4501 gcgactattt tcaagtctgg atagtgcgta agtcaccaga aagacacaac tttacatttt
     4561 gtagcatctg atttacggca tcttgccgct gttaaaaaat caggcacaat ttcttgtgcc
     4621 tgatagatat tacttgacgg gagcgggttg ttccgcgggt ctggcttttt caagcttcgc
     4681 cagatcgagt gcgatattca ccgtctcatc cagataagga tctggctcct ggtaatcttt
     4741 cggtagatca tccagtttct tcaactccgg tttaccttcg cgtttaaagc gttcgttcaa
     4801 acgcgccaga cgcgtcgcat catcttcatt attctctttc tcacgcacag cgtaattcag
     4861 agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc gcgatatcct tcatgatgtt
     4921 ctggaactca ggatctttcg cgatacgcgc attatgttcc ttcagcagct ccggttcaaa
     4981 ggccgttaaa tctcctgatt tcacataagt cgcggcatca atgctatccc acggcagcgc
     5041 gttatcttcg aatttctcac ccgtttccgt ttcttcatta cccgtcggca tgatgatgtc
     5101 tggcgttacg cctttacgtt gcgtactgcc gccgttaacg cgatagaact tctggatcgt
     5161 gtactgcaca gaacccagcg ctggccattc aggacgtaac atctgatcgt aaatacggtt
     5221 caatgaacgg tactgctgaa cggtgccttt accaaacgtc ggttcaccca caaccagggc
     5281 acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa gccgaagcac tgaagcggtc
     5341 aaccagcacc accagcgggc ctttatagaa gacctgtccg tcggtgtcgc tgtcttcacg
     5401 aaccttgccg ttgttatcgc ggacctgaac aatcgggccg gatggaatga acagaccgga
     5461 gagcgatacg gcttcagtca acgccccacc accattgcta cgcaggtcga taatcacact
     5521 gctgacattc tgtttttcca gtttctgcag ttgcactttg acatcgtctg tcaaacccac
     5581 atagaagcct ggaatatcca gcacgccgac tttctcttta ccgacggtct tcaccgacat
     5641 tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc aacgttacag tacgcgtctt
     5701 ggtcccttta ccagcaggta aaatttccag acgaacttta ctgcccttag gccctttaat
     5761 taaggccacc acatcatcaa gacgccagcc aatcacgtca accatcggct tgcctgtttg
     5821 accaacaccg acaattttgt caccaacgct gatagctttg ctcttcgctg ccggacctcc
     5881 tgccaccatc gaattgataa cggtgtagtc atcatccatt tgcagcactg cgccaatacc
     5941 ttccagcgac aaactcattt cagtgttgaa ctgttcggta ttacgcgggg aaagatagtt
     6001 ggtatgcggg tcgatttcac gtgcaaacgc cgtcattgcc agcgagaaaa catcttcgct
     6061 gttggtttgc gccagacgac gaatggcaaa tttgtagcgg cgagtcaggg tttcacgaat
     6121 ttccttatcc gtttttcctg tcagcttcag gcttaactcg tcgaatttga ctttactgtc
     6181 ccacagcgcg ttcaactcag cctcgttttt cggccagggc gctttgctgc ggtcaaggtt
     6241 ataagtgtcg ttgccggtga aatccatcgg cttttccagt accgacaaag cgtactggta
     6301 acgttcaaaa cggcgctttt gcgccagatt gtagagatcg tagaaaacgt cgagtttacc
     6361 tgaacgcagt tcatcgccta actcagtttt ctttttcgcg aactgttcaa catcgcttgc
     6421 cagcagcaca ttatggctgt aatcgagcag attcaggtag cggtcaaaga ttttggccga
     6481 aaatgcctga tcgaggtcga actggcgata atgagaacgg gtgaagcgcg acgttacgcg
     6541 ctcactcacc gtcgcatgct gcgtctcttc ctttaatacc ggaatttgat cagcacgcgt
     6601 gatatcttct acagcgaagg tctggcctgc tattgcaagc aggccagcta acgcggtaag
     6661 cctaaaaaac atgttcatgc ctggcccggc ctccgtttca gaacaccagg tgttctgcgc
     6721 gcacaatcaa agacataccc gaattcagct ggacgcggac gccgtctttg gtgatttcta
     6781 atacggtggc atccatcgcg ttttgacccg ctttcacctt cagggcttgt ccgacagtca
     6841 gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg aggtgctttt actgtttttg
     6901 gcgctttctc taccggcttt tgcgcacgag gtttacgttc agcgccttct ttgcggcgtg
     6961 gcgtggtcgg gcgtggcttg cgttcgcggc gcggtgcgtc ttctttctca ccagcagctg
     7021 cggcagcttc gcgttttttc gcttgctgtt cagcacgctg tgcctgaaca cgcgctttcg
     7081 cttcttcaag ctgcttgcga gcatgctcga catgttgctc gtccagctca ccgcatgggt
     7141 tgccgtcaag atcaacacgc gttgcgccgg atttaacacc gtaaagataa cgccagctcg
     7201 aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct caggttcatt tccccggcaa
     7261 cacgatcgac caaatcctga aaaataccga ttttcagcgg acgcgcttca ccttccgcac
     7321 tgaaacagtg gggaaaacgt tcggccagaa acgcgattac ttctttactg ctattcaact
     7381 taggttgatt ttccatgaaa tttcctgatt acaacggacg tagccaacaa gcgcaggcat
     7441 gaacaggcgt cattataatg acgctatcag taaatgctac gttatccgtt gattatcctg
     7501 cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag cactttttca agctgtgcca
     7561 caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg accgaagacg gtactatcga
     7621 tgtcgagaac accaataatc tgatttttca ccaccagtgg cagaacaatt tcagaattac
     7681 tcgccgcatc acaggcaata tgcccgtcaa acgcgtgaac atcctcgata cgctgcactt
     7741 gattgcgggc aaccgcagtg ccgcatacgc cgcgcccaac gggtatccgg acacaggcaa
     7801 ttttgccctg aaatggtccg agcaccagtg tatcgtcctc aagcaaataa aaacctgccc
     7861 agtttacgtc agtgagacgc tcatataaca acgcactggt gttcgcaagc gttgccagaa
     7921 aactggtttc tcccgccatc agcgcgttaa agtcgcgatt taaatccgcg taaaattctg
     7981 ctttgttcat tatataatca cttggttgtc ttacctggaa ctgccagcct attaaaataa
     8041 gcattaaatg cgttaatgct caagatcatt cccatcatgg gttaagatta atgttaattc
     8101 ttattacatt tggcacgtca tggctcttaa cacaccacaa attacgccga caaagaagat
     8161 aacagtgagg gctatcggcg aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg
     8221 tgacatgctg tttagcctgc ccgagataaa ttctcatcaa agtgcctatt gtccgcgctg
     8281 tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg cgcctggcgg caatggcctt
     8341 caccatgctg ttgcttatgc cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt
     8401 aggcattcgt attgacgcca acgttatgca aggcatctgg caaatgacca aacagggcga
     8461 tgcgataacg ggggcgatgg tctttttctg cgttatcggt gcccccctca ttctggtgac
     8521 ctccatagct tatttatggt ttggtaaccg attgggaatg aatttacgcc cggtactgct
     8581 gatgcttgag cggctgaaag agtgggtgat gttggatatc tacctggtcg gcattggcgt
     8641 tgcttctata aaggtacagg attatgccca tatccaggcg ggggtcggct tgttctcttt
     8701 tgtggcgttg gtgattttaa cgacggtgac gttgtcacat cttaatgtcg aagaactgtg
     8761 ggagcgattt tatccgcagc gccccgctac gcgaagggac gagaaactcc gtgtctgtct
     8821 tgggtgccat tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc
     8881 gctactcctg cgtcgccgtc atagtctgca aaaatgctgg gcggcgctgt tagcgtcaat
     8941 cgttttgttg ttacctgcca acctgttgcc tatctctatc atttatctga atggtggtcg
     9001 gcaggaagat acgattcttt ccggaattat gtcgctggca agtagcaaca ttgcagttgc
     9061 gggaatcgtg tttatcgcca gtattctggt accgtttact aaagtgatcg tcatgttcac
     9121 tttactgttg agtattcatt ttaaatgcca gcaaggttta cgcacacgca ttctgttact
     9181 gcggatggtg acctggattg gtcgctggtc gatgctcgac ctgtttgtca tatctttaac
     9241 catgtcgctg attaatcgcg atcagatcct cgcttttact atgggaccgg ctgcgtttta
     9301 tttcggcgca gcggtaattt tgactattct tgctgtggaa tggctggaca gccgcttact
     9361 ttgggatgca catgagtcag gaaacgcccg cttcgacgac tgaagcgcag attaaaaata
     9421 aacgccgtat ctcacctttc tggctgctgc cgttcatcgc gctaatgatt gctggttggc
     9481 tgatttggga cagttatcag gaccggggca atactgtcac catcgacttt atgtcggcgg
     9541 atggtattgt tccaggccgt acgcctgttc gttatcaggg cgttgaagtt ggaacagtgc
     9601 aggatatcag cctcagcgac gatcttcgta agattgaagt caaggtcagc atcaagtccg
     9661 atatgaaaga tgcgctgcgc gaagagacac agttctggtt ggtgacgcca aaggcctcgt
     9721 tggcaggtgt ctccgggctg gacgccctcg tcggtggtaa ttatatcggc atgatgccgg
     9781 gtaaaggtaa agagcaggat cactttgtcg cactcgacac ccaaccgaaa tatcggctgg
     9841 acaatggcga tctgatgatc cacctgcaag cccccgatct cggttcactg agcagtggtt
     9901 cattggtcta tttccgcaag atcccggtgg gtaaagtcta cgactatgcc atcaatccca
     9961 acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa
    10021 aaggtagccg tttctggaac gtttccggcg ttgatgccaa cgtcagtatc agtggcgcga
    10081 aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc gattgccttc gattcacctg
    10141 aagagtcgaa acctgccgaa gcggaagata cctttggtct gtatgaagac ctggcccata
    10201 gccaacgtgg cgtaataata aaactggaac tgccaggcgg tgccggatta accgccgact
    10261 cgacgccgtt aatgtatcag gggctggaag tcggacagct gactaaactg gatttaaatc
    10321 ctggtggtaa agtcactggg gaaatgaccg ttgatcccag cgtcgtcacc ctgcttcgtg
    10381 aaaatacccg catcgaatta cgtaatccaa aattatccct tagcgatgct aatctcagtg
    10441 ccctgctgac cggcaaaacc ttcgaactgg tgcccggcga tggcgagcca cgtaaggagt
    10501 tcgtggttgt gccaggcgaa aaagccctgc tgcaagagcc ggatgttctg acactgaccc
    10561 tgacagcacc agaaagttac ggtattgatg ctggtcagcc gctcattctt cacggcgtgc
    10621 aggtaggcca ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca
    10681 tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa atttgtcgtc aacagccgtg
    10741 tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg tgccagcgcc tcagaatgga
    10801 tcaatggcgg aatacgtatt ctgccgggcg ataaaggcga gatgaaagcc agctatccac
    10861 tgtatgccaa tctggaaaaa gcgctggaga acagccttag cgatttaccc accacaaccg
    10921 tgagtttgag tgcagagacg ctgccggatg tgcaggcagg atcggtagtg ctgtaccgta
    10981 aatttgaagt tggtgaagtt attactgtgc gtccgcgagc taacgcgttt gatatcgatc
    11041 tgcatattaa accggagtat cgcaaccttc tgaccagcaa cagcgtgttc tgggcagaag
    11101 gtggggcgaa agttcagctg aatggtagtg gcctgaccgt acaggcatcc ccgctctcca
    11161 gagcattaaa gggggctatt agcttcgata acctcagcgg tgccagcgcc agtcagcgta
    11221 aaggcgacaa gcgtattctg tatgcttccg aaacagcggc ccgtgcggtt ggcgggcaaa
    11281 ttacgcttca cgctttcgat gccggaaaac tggcggtcgg gatgccaatt cgctatctcg
    11341 gtattgatat cgggcaaatc cagacgctgg atctgattac cgcgcgcaat gaagtacagg
    11401 caaaggcggt gctctatccg gaatatgtcc agacctttgc tcgcggtggt acgcgcttct
    11461 cagtggtcac accgcaaatt tcggcagctg gcgttgagca tcttgatact atcctccagc
    11521 cgtatatcaa cgtcgaacca ggccggggca atcctcgccg cgactttgaa ttacaagagg
    11581 ccaccattac tgattcgcgt tacctggatg gcttaagcat tattgttgaa gcgccggaag
    11641 ccggttcgtt aggtatcggt acacctgtgc tgttccgtgg tctggaagta ggtacggtta
    11701 ccggaatgac gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc atcagtaaac
    11761 gctatcaaca cctggtgcgt aacaattccg tcttctggtt ggcatcgggt tacagtctgg
    11821 actttggtct gacgggcggc gtagtgaaaa ccggcacctt taaccagttt atccgtggcg
    11881 gcatcgcctt cgccacgcct ccgggtacgc cactggcacc gaaagcccag gaaggcaagc
    11941 acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga atggggaact gcgcttccca
    12001 aataatgccc actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac tgcacgcctg
    12061 tttttttgcc agtggtatat gctcgtggcc caacacaccg tttatttccc ggacgccttt
    12121 ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt ttgatgattt tcttgccgcc
    12181 tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc tgaaaacctc cgttgctgat
    12241 ttcctgcaat taaccgctcc ttatggctgg acgcttacgc caattccgtg gtgtgaagaa
    12301 ggtttctgga ttgaacgcga caatgaagat gcattgccgt tgggtagtac cgccgagcat
    12361 ttaagcggcc tgttttatat tcaggaagcc agttcaatgt tgcctgttgc cgccttgttt
    12421 gctgacggta atgcaccaca gcgggtgatg gatgtcgctg ctgcaccagg ttccaaaacg
    12481 acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc ttgccaatga gttttccgcc
    12541 agtcgggtaa aagtgttaca tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc
    12601 acgcattttg atggccgcgt atttggtgcg gcagtgccag aaatgttcga tgccattttg
    12661 ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac
    12721 tggtcaccag aaagcaatca ggaaatcgca gcgacccaac gggagctgat cgacagcgcc
    12781 tttcatgcat tacgtcctgg cggtacgctg gtttactcga cctgtacctt aaaccgggaa
    12841 gaaaacgaag ccgtttgcct gtggctgaaa gagacttacc ccgacgcagt agagttttta
    12901 ccgcttggcg atctcttccc tggtgcaaac aaggcgctga ccgaggaagg ctttttgcat
    12961 gttttcccac aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag
    13021 gcgatccccg tcttacccgc cccaaaatac aaagtgggca atttcccgtt tagcccagtg
    13081 aaagatcgcg aagccggtca aattcgtcag gcggctgcaa gtgttggctt aaactgggat
    13141 gaaaacctgc gactctggca gcgtgacaaa gaactgtggt tgttcccggt gggcattgaa
    13201 gccctgatcg gtaaagtccg attttctcgc ttggggatta aacttgccga aacgcacaac
    13261 aaaggttatc gctggcagca tgaggccgtt attgcccttg cctcccccga caatgtgaac
    13321 gcttttgaac tgacaccgca ggaagcggag gagtggtatc gcgggcgcga tgtatacccg
    13381 catgccgcgc cagtggcgga tgatgtattg gttactttcc agcatcagcc gattggttta
    13441 gccaaacgga ttggttcacg actgaaaaac agctacccgc gtgaactggt gcgcgacggg
    13501 aaacttttta ccggtaacgc ctgacagcgc acaaaaaagc gcactttttg actggcacat
    13561 tcgactgcct caactaggct gaaaaatggt gcgatcggac tggtcgtacc acaaccggca
    13621 gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat aggcgctttt gaaatcgacg
    13681 acggcgaatt acacggcgaa tcgccgggtg atcgaacgtt aaccattcct tgtaaatcag
    13741 accccgattt atgtatgcaa ctggatgcct gggatgctga aaccagtatc cctgccctgc
    13801 taaatggcga acactctgtc ctttaccgta cccgttacga tcaacaatct gatgcctgga
    13861 ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg gcgggttatt tgtccgggtt
    13921 attcccccgt tgtaaaatct ctcctaaact taacggtacg gcaccacact tcggggatga
    13981 aatgttcgcg ctggtacttt ttgtttgcta cctggatggc ggttgtgaag atattgttgt
    14041 ggatgtctac aacacggaac agcagtgtct ttattctatg agcgatcaac ggatccgcca
    14101 tggcggttgt tttccgattg aggattttat agatggtttc tggcgacctg cacaggagta
    14161 cggtgatttt taattattgc aattgcacaa gagtcagttc tcccccaaag acagcaccgg
    14221 tatcaatata atgcaggttg ccaatatcca cgcgatggcg caaaggtgta tgaccaaacc
    14281 agaaatgatc agcacctgca attccctgcc ctttttggcg ttcacctaat cgcgagcggc
    14341 tccacaagac ctgatgcaaa tcgacgtcct tttgccattc ataaacatca tctggataat
    14401 cggcatgagc aataacatgt ttgccggtgc ggctgtgtac ttcaagaata aagggcaaat
    14461 gctgacattt ttccagcgcc gttttcgctt gtttctgttg attatctgcc agcgcaataa
    14521 accagtcgcc gccattcatc aaccataaag acatctgccg ggatgccagc gcatccatcg
    14581 ccatctgttc atgattgcct cttaccgcac gaacccaatg ttgttccagt aactgcaggc
    14641 aacgtaaact ttgcggccca cgatcgataa cgtctcccac tgagataagt aaatctcgcc
    14701 acggatcaaa acgacaatgc cataatttgc ggcgcaattg ctcaagacaa ccgtgtatat
    14761 cgcctgaaag ccagatatgt cgccattgat gacccgcaat tctctgataa acgggcgcag
    14821 gctgtttcat caatattttc ctcccgcgct aaagatcaca taatcttaac aagaatgtta
    14881 aaaaacgctg gactcaaaca gtagagtgtg tgttatggtt gactataaag tcagcgaagg
    14941 gaatgcttct ggcttttaac agataaaaag agaccgaaca cgattcctgt attcggtcca
    15001 gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg gcattaatgc aggcttagtt
    15061 gccttgctct ttaagaatag atgacgacgc caggttttcc agtttgcgtg caaaatggtc
    15121 aataaaaagc gtggtggtca tcagcttaaa tattaaaaac cgcccgttct ggtgaaagaa
    15181 ctgaggcggt tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca
    15241 gctcgctcta taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc
    15301 tgaatcaccg aaatgggttg ggctttagtc ttcccactgg caacttcctt ttgtgcgata
    15361 tcgtttaaag gatactgcac gagagtactt gggttaatga catacaaagc attacccggt
    15421 cggcaagtca gcatcacctc ttcgcgatta aacgcccatt tgtctttacc tacttcaaaa
    15481 cggctgacgg taatcacctg cggtgccgcc agcgcagctg cagaactggt gagtaacaga
    15541 aacgccagaa tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca
    15601 agcaggctga tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccag
    15661 acaaaccagg gagccgcacg atcttcatcc tgcctcatgc gcggtacaag aacataacga
    15721 ttagccagcg cgatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg
    15781 ccccagtgag tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg
    15841 cttgccagta cgccgatcac cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga
    15901 atagcctggt gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat
    15961 cccccaaacc acgccgccgc acagatcagg tgaattgcat gattagtctg atggattttc
    16021 gccgttaccc cttcattcag cgtcgcatga ccgactcccg ccagcagaat aaattgcgcg
    16081 gtagtgagca taaatagcaa acgtggcata ttctgcggtt gcataagggc gacgatcaat
    16141 gttacgaggg cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca
    16201 gccagccaaa tgtttggcga aaatacatct gaccatcccg ttcccatcaa cccgccctga
    16261 acggcgagca ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc
    16321 agaaaacgct tcgtcagcaa gcgacgaatc gttaaggggg ccagccaggc accgtacatt
    16381 gcgaagccaa aaaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta
    16441 aacgccagca tgatttattt cacgctaaag gtgtaatgtc ctttcgtttt atgcccatcc
    16501 accgaaacaa catgccagtc aacggtgtag gttccgggtt tcagcgaatc ggccagtgga
    16561 acaatcagtt gcttttggtc ctgctcatta cgcttcgccg gtaatgtttt aatattttcg
    16621 ttttttggcc ccgtgatttt tgcgccactg aatcctgttt caacgccttc cgagaagttt
    16681 aaagtgattg cctgcggagc tgctgtcact tgtgcatttg ccgcaggata ctgatgcgtt
    16741 aaatgcgcat gtgcccagac ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg
    16801 tagcgaaggg agcgtgcagt tgaagccata ttatctattc ctttttgtaa taactttttt
    16861 acagagcata accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcaa
    16921 ggctcactct tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc
    16981 tggctaaact ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggcagggg
    17041 tggcatttaa agaacgctac aatatgccag tgatcgctga agcggttgaa cgtgaacagc
    17101 ctgaacattt gcgcagctgg tttcgcgagc ggcttatcgc ccaccgtttg gcttcggtca
    17161 atctgtcacg tttaccttac gagcccaaac ttaaataaaa cttatacata gttacacttt
    17221 cttacataat gcctgctaaa ttatgagtat tttctaaacc gcccccataa tttgcagtca
    17281 ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg
    17341 taaaacctca ctcaccgaac aggtagctca tcatctggag tttgtgaggg ccgccgccag
    17401 gcaacaatgt cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg
    17461 acgtgccctt cctgccccac ccgatctctc gctattagac ccgctttgtt atgccgcaac
    17521 gacctggcga atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg
    17581 tggaattgcg gtattcgcac cgtggcgaaa aacgccaggg atctaccatc agagttatgg
    17641 tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc gatgaacaac cgcaaggtat
    17701 ggatatggac ccgacctgtt cactgtttac gacagggcaa tgtctgggag aacccgacct
    17761 gctggcgtcg gcccgtcgct tacaattttt ttcacatcag tactcgattg ctgtgctgat
    17821 ggcaaatgcc cgtggtaaca gtgcactatg ggatgaacat ggtcgtctca tcgttcgcgc
    17881 cgatcgtggt tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat
    17941 cattccatta cgctaggctt tttcggcctg gagcatgcca tgttgcgcat tatcgataca
    18001 gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga
    18061 aaaatcgtca atcccatgag ccacctggtg cgtcccgatc gtcctattag tccacaggcg
    18121 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
    18181 atcccgcact attacggtag tgaatggtat gtcgcgcata acgccagctt tgaccgccgc
    18241 gtactgcctg aaatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg
    18301 ccagggatca agtacagcaa tatggcgtta tataaaacac ggaagctcaa tgtacagacg
    18361 ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc tgcgttgctt
    18421 atcgatatta tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt
    18481 ccatcgttga tgacgacttt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
    18541 gccgaacgcg atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg
    18601 gagctgcgtt taacactgaa acattatctg gaaaatactt aatccgcagg cgtagcgggt
    18661 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
    18721 ttaaagcgac cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga
    18781 tggttatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
    18841 acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
    18901 ttgtcatacg ggctgtagct tttcatatac tcgtaatatt gcggatcctg cggattcccc
    18961 cactcttcaa attcgccggt ggtaagagga attgattcat ccagcattgt tgttacaaca
    19021 tcaacaaacg gcacctgggc gataacgccg tggaacaatt ccgggcgttg attaatcgca
    19081 acgcccatca acataccccc cgcactcccg cccatcgcat aacagagcga aggagagcca
    19141 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc
    19201 ttcagaaatt taccgtcctc gtaccattgt tgccccagct caccgccgcc acgaacatgg
    19261 acaatggcgt agacaaatcc acgatccaac aaactcaagc ggctaaaact gaaatcggca
    19321 tcaatacttg cgccgtaaga accgtagcca tacaccagca gcgggttgtg tcctttgcga
    19381 aaatgtttgc gatgatagac cagcgaaacc ggaacttcga cgccatcacg ggcgactatc
    19441 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt ttgttttaat
    19501 acacgacgct caccggtatc catatccagt tcaaacaaag tatctggtgt ggtcatggac
    19561 gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca
    19621 taggccggat catcaaaggc aatgccaatg acttcccggg tcttgcggtt aatttgccgc
    19681 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
    19741 ccttccagca taatgttatc acgtggcgga attaactctt cccactgttg ctcatcacgc
    19801 atacgggtac ggtataagcc aaagttttta ccgtggcggt tggaacgcag ataaaaccga
    19861 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc
    19921 tcggcatcgg ctatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaga
    19981 tgaatgacta catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct
    20041 ttttcttcgt agatcagttt atcctgcgat gctggcgtac cgatggcgtg acgccagacc
    20101 tgataaggaa gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggaatcattt
    20161 gcccagacaa agctggggtc aacgttatcc agcagttccg gataccagtt acctgtttcc
    20221 agattacgaa aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagtgccata
    20281 atggtgttat cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagccgcg
    20341 cgcttgttgg catcgagcaa tgtttcccac tcatcccact cttcgctgaa cgccgattga
    20401 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
    20461 tttatgtagg gcgcagaaac ttctctttgc ggaatgcggt cgatgatttc ctttaagatg
    20521 cgatcctgca aggcttgttg cgaggccatc acccgatgac cgtaactatt ttcttgttgc
    20581 agatagtcca ggacttccgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg
    20641 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
    20701 ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag
    20761 cgaaacaggg tagtcattgc ttagatgatg acaggtaatg gcgcggatat cgactgttat
    20821 gcagacagag aaaatcagcc tgttcaaatg gctgtgcggt tctggataac ccgaaatagt
    20881 caacttcagg ctatccagag agcggaatta ctccgccaaa gtacgttttt gctgttcgag
    20941 atcgcgttca atgccttcac gaacgtcctg ggggattttc agcgcatcac ccagtgcatt
    21001 caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcagctca ggaaatagat
    21061 ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg
    21121 ttcgattgcc tgctcaatga gtacacgccc cttctcttcc acaccggctt cacgcaattg
    21181 caggtcgatt gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac
    21241 cagcgcaagg atcaaacgtt ccgtacgctc atccagcggc gtactttgcg cgccaaactg
    21301 cggttcgtcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc
    21361 cgctaccgct ccgccgccaa ccagtaacgc gtttgtgcca tatttcgtaa gaagtttacg
    21421 tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgccag gcactaaaag
    21481 tttgcccaat ccctgatccg cagacgagga ggtagaagaa cggctttgcc caagcaggga
    21541 ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt
    21601 agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg
    21661 gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtgttcact gtatataagg
    21721 tgataagggt attttaggtg aaagtagttt gacattttga gtggaactgt ttgatgctga
    21781 ttgcccggtt cgacgctact gcgtcttctc aggcctacga ttgtgttgcg ttgtagggcg
    21841 gacaaggcac ccgcgccgca tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc
    21901 cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca
    21961 cttttaccac gcacggttag cggtactgac catttatcat ctttcccctg aatgtcctgc
    22021 aaactgaccc atgccacggg atcggcctga ccgacaattt tttgatcatc agcccagcgc
    22081 gccacgcgat tttgttggta atcacgtttt acgctcgcgg caattccggc agcatccaga
    22141 ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat
    22201 gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc
    22261 atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag
    22321 gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat
    22381 ttgccggttt tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg
    22441 gcttcacgtt gctcgtcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga
    22501 tcggccgtat caagcatctt gtcataagca tcggcttcct ttttgctggt aaacgacatt
    22561 ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca
    22621 taataactgt ggttttatac agtatatttc ttttcagttg ggaaatcaac atcagcaata
    22681 aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt
    22741 atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc
    22801 ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc
    22861 gtcgatcgct atgccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat
    22921 atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg
    22981 ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat
    23041 gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg
    23101 gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaaacctt
    23161 ttccgcgaag cggttgccgc cattggctac ccctgtattg ttaaaccggt gatgagttct
    23221 tccggtaaag ggcagacgtt tattcgttct gcagagcaac ttgctcatgc atgggagtac
    23281 gctcagcaag gtggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt
    23341 gacttcgaaa ttaccctgct aaccgtcagt gcggtggatg gcgtccattt ctgtgcaccg
    23401 gtaggtcatc gccaggaaga tggcgactat cgtgaatcct ggcaaccaca gcaaatgagc
    23461 ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc acttggcggt
    23521 tatgggctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaagtc
    23581 tcccctcgcc cgcatgacac cgggatggtg acgctaattt ctcaggatct ctcggagttt
    23641 gccctgcatg tacgtgcctt cctcggactt ccggttggcg ggatccgcca gtatggtcct
    23701 gctgcttctg ccgttatcct gccgcaactg accagtcaga atgtcacgtt tgataatgtg
    23761 cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc
    23821 agccgtcgtc tgggggtagc actggctact gcagagagtg ttgttgacgc cattgaacgc
    23881 gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca
    23941 aaaatgcccg atcctcgatc gggcattttg acttttacag cttagcgcct tctacagctt
    24001 cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct gccggaacca
    24061 gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg
    24121 gagaaatacc acctgtcggg cagaaacgga cctgggagaa cggacccgcg atcgcctgca
    24181 gggctttcac accgccgtta gcttcagccg ggaagaattt gaactctttc aaaccgtagt
    24241 ccatacccag catcagttcg gaaacagtgc tgatccccgg aatcagagga atagtccctt
    24301 cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcaaac tgtgcacctg
    24361 cttcagtgac ttctgccagc tgctgtggat tcagcaccgt accggcaccc acaatcgctt
    24421 caggcacttc tttggcgata gcacggatag cgtcaactgc gcactcggta cgcagagtca
    24481 cttccagaac gcgcacccca ccagcaacca acgcttttgc tatcggcacc gcgtgttcca
    24541 gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg
    24601 ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag
    24661 tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt
    24721 cacgtcctgt tcccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca
    24781 gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt
    24841 ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ctctggtgtt acgtggatag
    24901 ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga
    24961 aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt
    25021 tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagt aaacccgctt
    25081 caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct
    25141 ggttctctac cggcacggca gaggttttca taaccgctcg ccccaggtta ccgcttaaca
    25201 cttttgtccc accatgatga gagaaaggtt gttcgaagga cgcgatcaca ttgttgtcga
    25261 gtgatttttc tgccccttcc cgccagtcca gttcaccatt attcagccat ggttcaaggg
    25321 tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt
    25381 tgagcagttc acgtaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg
    25441 ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt
    25501 catcccagtt aatctgaata ccggctgcgc gcgccatcgc cactaggtgc atggtgtggt
    25561 tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga
    25621 tcatcttacc gatcggcatc cattcattac cattaccggt catgcgcgta acctggcgcg
    25681 cagctgcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg
    25741 gcaactgcat ccccataaac tccaccacca tctggttggt gttggcagta ccgtagaaag
    25801 tacatgttcc cggcgcatga taagacgcgg cttctgactc cagtaatgcc atgcggtcca
    25861 ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg
    25921 ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aaacgacagg gctgccatgg
    25981 tcagacctgg gacaatcttg tcgcacacac cgagaaacag agcaccatca aacatgttat
    26041 gggacagccc caccgccgca gacatcgcaa tcacttcgcg gcttaacagc gacaattcca
    26101 ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac
    26161 caaccgcatt cgcttcatgc agggctttac gaatgatttc tggatagtgt tcataaggct
    26221 ggtgagcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc
    26281 ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc
    26341 atgccaactg cgaacgatga acggtcgaag ttttcgcttg ttctatccgg gcgaggtaag
    26401 cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac aattgtgggt
    26461 tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacgc gtttcataca
    26521 tggaaattat gattgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag
    26581 tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc
    26641 tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggatgag cgcagatatt
    26701 actcaaactc attccaggaa cgaccatcac gggtaatcat cgccactgag gcaacgggtc
    26761 cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa
    26821 tggagtctac ccatttccag gcttcttcca cttcgtcgcg acgtacaaac agtgcctgaa
    26881 taccacgcat ggtttccagc agcaaacgtt cataggcatc cgccagatgc gtctgattaa
    26941 aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc
    27001 caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca
    27061 gtttattttg cggtagatcc tgccacgact ctttaaacag attcagttca ggtgttttga
    27121 aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg
    27181 gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgccacgaag gtttctgtat
    27241 tgctgctctt gttcgcgccc tcttcttcca gatagcccgg cacttttttg ccctgagcga
    27301 agcccgcagt atattgcccg cgtacggttt tttcgcgcac attggagcgg tcgatgcggc
    27361 gcagagactt cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagacg
    27421 gcggagacat cgcaatcatg caaagaattt gcagcaggtg gttctggatc atgtcgcgca
    27481 tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg
    27541 taatctcaac atgatcaatg gtgcgattgt cccagttgtt cacaaacaga gagttagcaa
    27601 aacgcagcgc caacaggttc agcactgttt ctttaccaag atagtggtcg atacggtaaa
    27661 cctggcactc ctcgaagtat tcgccaacct gatcgttgat ttcctgcgag gtcgccagcg
    27721 acgtccccag cggtttttcc atgactacgc gtgccggttt agcattcagt tttgcctcac
    27781 caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga
    27841 tacgattttt ttgatccagc atcgcgccga gacggctgaa tgcagcagtg tcattgacat
    27901 caagattaca aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt
    27961 ctttcatgaa agtttcgagc gcctcgcgga caactttggt ataagccgct ttatcccagt
    28021 cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt
    28081 gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca
    28141 ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc
    28201 ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag
    28261 cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac ggtaaaagcg cgcaaagtgc
    28321 ggcaaaactg ataaaaaaat catcgttttc cattgggtaa aaatctgaca ctgatcatgt
    28381 tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacagca cacaggcgta
    28441 atatctgaca aaactgcatt tcgatttctt tcagagcgga aatcgtcatt acccgtgagt
    28501 ctctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat
    28561 ctgagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta
    28621 ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca
    28681 gcatggacac acgcggtttt cctgatttta aacttcatct ggcacaaagt ctggcgaatg
    28741 gcactcccta tgttaatcgc aacgtcaatg aagatgacag cgttgaatca tacacgggga
    28801 aaatatttga gtccgcaatg gcaacgctcg atcatgtccg tcattcactg gataaatctg
    28861 ccatcaaccg cgccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag
    28921 gttcttcagc cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg
    28981 tggtgtactc cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag
    29041 acgtggtggt gctgatttct cacacgggaa gaacaaaaaa tctggtcgag ctggcgcagc
    29101 tggcacgcga aaacgacgct atggtaattg ccctcacctc tgcgggtacc ccactcgccc
    29161 gggaagcaac gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca
    29221 tggtttctcg acttgcacag ctgaccgtga tagatgtgct ggcgacagga tttactttgc
    29281 ggcgcggtac aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc
    29341 gttttgataa gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct
    29401 aatcttatac gacatccgaa tgagattaat ttatcgccat cacggcgtta tttcattcgg
    29461 atttcatgtt caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg
    29521 cttcgcagaa ctaaaatcgt taccacgtta ggcccggcaa cagatcgcga taataacctt
    29581 gaaaaagtta tcgcggcggg tgccaacgtt gtacgtatga atttttctca cggctcgcct
    29641 gaagatcaca aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat
    29701 gtggctattc tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc
    29761 aaagttttcc tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa
    29821 ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt tgtgcctggt
    29881 gacatcctgc tgctggacga tggtcgcgtc cagttaaaag tactggaagt ccagggcatg
    29941 aaagtgttca ccgaagtgac cgtcggtggt cccctctcca acaataaagg tatcaacaaa
    30001 cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact
    30061 gcggcgttga ttggcgtaga ttacctggct gtctccttcc cacgctgcgg cgaagatctg
    30121 aactatgccc gtcgtctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt
    30181 gaacgtgcgg aagccgtttg cagccaggag gcaatggatg acatcatcct cgcctctgac
    30241 gtggtaatgg ttgctcgcgg cgacctcggt gtggaaattg gcgatccgga actggtcggc
    30301 attcagaaag cgttgatccg tcgtgcacgt cagctaaacc gcgcggtaat cacggcgacc
    30361 cagatgatgg aatcaatgat tactaacccg atgccgacgc gtgcagaagt catggacgta
    30421 gcaaacgccg ttctggatgg tactgacgct gtgatgctgt cggcagaaac tgctgctggt
    30481 caatacccgt cagaaactgt tgcagccatg gcgcgcgttt gcctgggggc ggaaaaaatc
    30541 ccgagcatca acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagcc
    30601 attgccatgt cagcaatgta cgcggctaac cacctgaaag gcgttacggc gatcatcacc
    30661 atgaccgaat cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctgccaatt
    30721 ttcgccatgt cacgacatga acgtacgctg aacctgactg ctctctatcg cggcgttacg
    30781 ccggtgcact ttgatagcgc taatgacggc gtagcagctg ccagcgaagc ggttaatctg
    30841 ctgcgtgata aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg
    30901 atgagtaccg tgggttctac taataccacg cgtattttaa cggtagagta agtacgttgc
    30961 cggatgcggc gaaaacgcct catccggcct acagttcaat gataattcaa cagatttcga
    31021 atcttctgaa gcaaacttga acttatcatc agtcgaaggc ctctcctcgc gagaggcatt
    31081 ttttatttga tgggataaag atctttgcgc ttatacggtt ggatttcgcc cggtttgcga
    31141 gttttcagca attttaatat ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg
    31201 acttcttcat tcatccgccg cgcaatcgta tgatcatccg cctctaacag atcatccatc
    31261 ggtgggcgca cctgaatcgt cagacgatgc gtcttgccat cataaatcgg aaacagcggt
    31321 acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag
    31381 gtggcaaaga aatcaacaaa ttcgctgtgt tctgggccat gatcctgatc gggtaaataa
    31441 tatccccagt aaccctgacg taccgactgg atgaatggtt taataccatc atttctcgca
    31501 tgcagacgac caccaaagcg acggcgcacc gtgttccaga cataatcaaa aaccgggttg
    31561 ccctgattat ggaacatcgc tgccattttc tgcccttgcg aggccatcag catggcagga
    31621 atatcgacgg cccaaccgtg cggcaccaga aaaatcactt tctcgttatt acgccgcatc
    31681 tcttcgatga tctccagccc ttgccagtca acgcgcggct gaattttctc cggcccgcgt
    31741 attgccaact cagccatcat taccatcgct tgcggcgcgg tggcaaacat ctcatctaca
    31801 atcgcttcgc gttcagcttc actacgttct ggaaagcaga gcgacagatt gattaacgca
    31861 cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga
    31921 tcacggaact ttggcggcgt taaagcgata cccgccatcg ctgctacgcc cagccatgct
    31981 ccccagtagc gcgggtggcg aaaggattta tcaaactcag gaatgtattc gctattattt
    32041 tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt
    32101 agcggcgcaa cttgccccgc accaaataaa aaagccggta ctgactgcgt accggctgcg
    32161 aatggatgtt aattaatcaa accgtagctg cggcacaatc tctttggcct gtgccaggaa
    32221 ttcgcgacga tcggagccgg tcagcccttc ggtacgcggc agttttgccg tcagcgggtt
    32281 tacggcctgc tggtttatcc atacttcata gtgcagatgc ggcccggttg aacgtccggt
    32341 attaccggaa agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt tcaccaggat
    32401 cttgcgcaag tgcatataac gcgtggtgta gctgcgacca tgacgaatag ccacataata
    32461 acctgctgcg ccactacgtt tggcaaccac cacttcaccg tcacccactg aaagcactgg
    32521 cgtaccttgt ggcatggcaa aatcaacacc tctgtgtggc gcaacgcgac cggtcaccgg
    32581 attagtacga cgcgggttaa agttagatga gatacggaac tgtttcgccg tcgggaatcg
    32641 caagaatcct ttcgccagac cagtaccgtt acgatcatag aatttgccat cttcagcgcg
    32701 gattgcataa taatctttac cttctgaacg caaacgtacg cccagcagct ggctttgctc
    32761 acgtttacca tcaagcattt ctcgtgacat taacaccgca aattcatcgc cttttttcag
    32821 tttgcggaaa tccatttgcc actgcatggc tttaatcact gcgctcactt cggcgctggt
    32881 taaaccggcg tttctggcgc tggcaacaaa gcttcccccg acggtacctt tcagcagatt
    32941 gttgacccac tctccttgct gcatttcgct ggtcatttta aaaccgttag cggcagtacg
    33001 gtcataggtt cgggtttcac gacgagacac ttcccaggtg aggcgctgca gttcgccgtc
    33061 cgcggttaat gtccaggaga gttgttgacc gattttcagg ttacgcaatt ctttgtcggc
    33121 agcagccagt tgggtgatat cacccatatc aataccatac tgattgagaa tgctgcttag
    33181 cgtatcgcca gtggaaacaa catattcatg cacgcccgct tcaccggcga ttttgtcatc
    33241 cagttcgtcc tggggaatgg cttcatcttc ttgtgcagct tgatcaatcg gctcactggc
    33301 ttcaggtaag agcgaacgaa tttcgttctg ttccagctca atggttttga caattggcgt
    33361 ggcatcacgg tgataaacat agggccgcca gacagcgacg gccagagtaa gaacggtgag
    33421 cgaccccaac ataacgcggt gtggtcgcgg taaattatta aacgccaggg cgacagagcg
    33481 ggctatctgt tgcacgtaat cacttcctca ttaatctcct ttcaggcagc tcgcatactg
    33541 gttggctaat tgattcagga attctgaata gcttgtttta cccagtttga tattcgtccc
    33601 caggggatcc aacgttccca tacgaacgga tgtccctcgt gcgacgctct caacgaccgc
    33661 tggcctgaac tgtggctcag caaaaacgca ggttgctttt tgctcaacca actgtgttct
    33721 tatttcatgt aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa aatgaccaag
    33781 cggtgtcagt ccgaactgtt tttcgaagta gccgtaagca tcgtgaaaaa cgaaataacc
    33841 tttccccttg agcggcgcga gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc
    33901 ctcaaaatcc ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa gttccactaa
    33961 ttttccatgg attgcaaccg ctgtagcccg cgctatctct ggggaaagcc aaagatgcat
    34021 gttgaaatcg ccgtgatggt gatcttcgtc acttttttcc gcgtgatcgt gatcatcatc
    34081 atcgtcgtga atacttttca tcagcagcgg tttcacatct tctagctgcg caatcgttac
    34141 ctgtttcgct tctggtaatt tacttaccgg tttttgcatg aacgcttcca tctccgggcc
    34201 aacccaaacg actaagtccg cgttctgtaa gcgttttaca tccgatggac gcagtgaata
    34261 atcatgttct gaagcgccgt caggcagtaa aacctgcgtt tctgttaccc catcagcaat
    34321 ggcagaagcg atgaacccaa cgggtttaag cgaagcgaca acggcggcat ctgcggcctg
    34381 tgttgcaccg ccccagagag cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa
    34441 cataatgcga ccaatcatcg taatgaatat gagaagtgtg atattataac atttcatgac
    34501 tactgcaaga ctaaaattaa catgacaagt ctggtttccc tggaaaatgt ctcggtttct
    34561 tttggccaac gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg
    34621 actttacttg ggccaaacgg cgcaggtaag tcgacactgg tacgggtagt gctcggactg
    34681 gtaacacccg atgaaggggt tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg
    34741 cagaagctgt atctcgacac cacgttgcca ctgaccgtaa accgtttttt acgcttacgc
    34801 cctggcacac ataaagaaga tattttgcct gcactgaaac gtgtccaggc cgggcatctg
    34861 attaacgcac cgatgcaaaa gctctcgggt ggcgaaacgc agcgtgtact gttagcgcga
    34921 gcattgttaa atcgaccgca attattagtg ctggatgaac ccactcaggg cgtggatgtg
    34981 aatggtcagg tggcgttata tgaccttatt gaccaactgc gtcgtgaact ggattgtggc
    35041 gttttaatgg tatctcacga tctgcatctg gtaatggcaa aaaccgatga agtgctttgc
    35101 ctgaatcacc acatttgttg ttccggcaca ccggaagttg tttccctgca tccggagttt
    35161 atttctatgt ttggtcctcg tggtgctgaa caactgggta tctatcgcca tcatcataat
    35221 catcgtcacg atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg
    35281 aattattatt tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg
    35341 gttcgtttgt agtctggcgt cgtatgtctt atttcggtga tacgctggct catgcctcat
    35401 tacttggcgt cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg gtgattgcag
    35461 ttacgctgct gctggcgggc ggtctggtat ggctcgagaa gcgtccacag ctggcgatcg
    35521 acacgttatt agggattatg gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc
    35581 tgatgtctaa tattcgtgtt gatttgatgg cttacctgtt cggtgattta ctggcagtga
    35641 cgccagaaga tctcatctct attgcgattg gcgtggtcat cgtggtggct attttgttct
    35701 ggcaatggcg caatttgctg tcgatgacga ttagcccgga tctggcgttt gttgatggtg
    35761 tgaaattaca gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg attggtgtag
    35821 cgatgaaatt cgtcggcgcg ttgattatta cttcactgct gattattcct gctgctactg
    35881 cacgtcgctt tgcccgcacg ccggaacaga tggctggtgt cgctgttttg gtggggatgg
    35941 tggcagtgac tggcggttta accttttccg cattttacga tacacctgca ggcccgtcgg
    36001 tggtgctatg cgcggcactg ttatttatta tcagtatgat gaaaaaacag gccagctaat
    36061 ctttcgctga acatatttgt cggatgcggc gcgagcgcct tatccgacct tgtattatcc
    36121 ctccagtgca gagaaaatcg gtcagttttc tctgcctgca gtccgcatgc cttatccgac
    36181 ctacggttcg ctatctctgg taggcctgat aagacgaac
//
LOCUS       DACADA010000032        34003 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.032, whole genome shotgun sequence.
ACCESSION   DACADA010000032 DACADA010000000
VERSION     DACADA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.032"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            184..1278
                     /gene="gcvT"
                     /locus_tag="ISB17_001572"
     CDS             184..1278
                     /gene="gcvT"
                     /locus_tag="ISB17_001572"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAP1979516.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1302..1691
                     /gene="gcvH"
                     /locus_tag="ISB17_001573"
     CDS             1302..1691
                     /gene="gcvH"
                     /locus_tag="ISB17_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAP1979517.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1810..4683
                     /gene="gcvP"
                     /locus_tag="ISB17_001574"
     CDS             1810..4683
                     /gene="gcvP"
                     /locus_tag="ISB17_001574"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAP1979518.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLIRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4849..6282)
                     /locus_tag="ISB17_001575"
     CDS             complement(4849..6282)
                     /locus_tag="ISB17_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP1979519.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6327..6638
                     /locus_tag="ISB17_001576"
     CDS             6327..6638
                     /locus_tag="ISB17_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP1979520.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6802..7461
                     /locus_tag="ISB17_001577"
     CDS             6802..7461
                     /locus_tag="ISB17_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP1979521.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(7538..8518)
                     /gene="ygfZ"
                     /locus_tag="ISB17_001578"
     CDS             complement(7538..8518)
                     /gene="ygfZ"
                     /locus_tag="ISB17_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAP1979522.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARTALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8761..9027
                     /gene="sdhE"
                     /locus_tag="ISB17_001579"
     CDS             8761..9027
                     /gene="sdhE"
                     /locus_tag="ISB17_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAP1979523.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9008..9415
                     /locus_tag="ISB17_001580"
     CDS             9008..9415
                     /locus_tag="ISB17_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979524.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9455..9976)
                     /gene="fldB"
                     /locus_tag="ISB17_001581"
     CDS             complement(9455..9976)
                     /gene="fldB"
                     /locus_tag="ISB17_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAP1979525.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10088..10984
                     /gene="xerD"
                     /locus_tag="ISB17_001582"
     CDS             10088..10984
                     /gene="xerD"
                     /locus_tag="ISB17_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP1979526.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11009..11719
                     /gene="dsbC"
                     /locus_tag="ISB17_001583"
     CDS             11009..11719
                     /gene="dsbC"
                     /locus_tag="ISB17_001583"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAP1979527.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11725..13458
                     /gene="recJ"
                     /locus_tag="ISB17_001584"
     CDS             11725..13458
                     /gene="recJ"
                     /locus_tag="ISB17_001584"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP1979528.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13549..14647
                     /gene="prfB"
                     /locus_tag="ISB17_001585"
     CDS             join(13549..13623,13625..14647)
                     /gene="prfB"
                     /locus_tag="ISB17_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP1979529.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14657..16174
                     /gene="lysS"
                     /locus_tag="ISB17_001586"
     CDS             14657..16174
                     /gene="lysS"
                     /locus_tag="ISB17_001586"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP1979530.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16217..16765)
                     /gene="idi"
                     /locus_tag="ISB17_001587"
     CDS             complement(16217..16765)
                     /gene="idi"
                     /locus_tag="ISB17_001587"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP1979531.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16820..16891
                     /locus_tag="ISB17_001588"
     CDS             16820..16891
                     /locus_tag="ISB17_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979532.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16888..17013)
                     /locus_tag="ISB17_001589"
     CDS             complement(16888..17013)
                     /locus_tag="ISB17_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979533.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17015..18463)
                     /locus_tag="ISB17_001590"
     CDS             complement(17015..18463)
                     /locus_tag="ISB17_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP1979534.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18899..20818
                     /gene="ygfT"
                     /locus_tag="ISB17_001591"
     CDS             18899..20818
                     /gene="ygfT"
                     /locus_tag="ISB17_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAP1979535.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20818..21306
                     /locus_tag="ISB17_001592"
     CDS             20818..21306
                     /locus_tag="ISB17_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1979536.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(21342..22709)
                     /locus_tag="ISB17_001593"
     CDS             complement(21342..22709)
                     /locus_tag="ISB17_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1979537.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22745..24061)
                     /gene="guaD"
                     /locus_tag="ISB17_001594"
     CDS             complement(22745..24061)
                     /gene="guaD"
                     /locus_tag="ISB17_001594"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HAP1979538.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24079..25479)
                     /gene="xanQ"
                     /locus_tag="ISB17_001595"
     CDS             complement(24079..25479)
                     /gene="xanQ"
                     /locus_tag="ISB17_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HAP1979539.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGISYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25644..28514)
                     /locus_tag="ISB17_001596"
     CDS             complement(25644..28514)
                     /locus_tag="ISB17_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HAP1979540.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28511..29290)
                     /locus_tag="ISB17_001597"
     CDS             complement(28511..29290)
                     /locus_tag="ISB17_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HAP1979541.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29341..30669)
                     /gene="ssnA"
                     /locus_tag="ISB17_001598"
     CDS             complement(29341..30669)
                     /gene="ssnA"
                     /locus_tag="ISB17_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAP1979542.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFTRQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIVLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30672..33770)
                     /gene="ygfK"
                     /locus_tag="ISB17_001599"
     CDS             complement(30672..33770)
                     /gene="ygfK"
                     /locus_tag="ISB17_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HAP1979543.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKSKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         8324 a         8611 c         8974 g         8094 t
ORIGIN      
        1 ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca gtctgtttcg
       61 gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc tccgatgctg
      121 ggtttcgtgg tgataatttc gccatagaaa agttgtcagc cccgcttatt caatgaggac
      181 aagatggcac aacagactcc tttgtacgaa caacacacgc tttgcggcgc tcgcatggtg
      241 gatttccacg gctggatgat gccgctgcat tacggttcgc aaatcgacga acatcatgcg
      301 gtacgtaccg atgccggaat gtttgatgtg tcacatatga ccatcgtcga tcttcgcggc
      361 agccgcaccc gggagtttct acgttatctg ctggcgaacg atgtggcgaa gctcaccaaa
      421 agcggcaaag ccctttactc ggggatgttg aatgcctctg gcggtgtgat agatgatctc
      481 atcgtctact actttactga agatttcttc cgcctcgttg ttaactccgc cacccgcgaa
      541 aaagacctct cctggattac ccaacacgct gaacctttcg gcatcgaaat tactgttcgt
      601 gatgaccttt ccatgatcgc cgtacaaggg ccgaatgcgc aggcaaaagc tgccacactg
      661 tttaatgacg cccagcgtca ggcggtggaa gggatgaaac cgttctttgg cgtgcaggcg
      721 ggcgatctgt ttattgccac caccggttac accggtgaag cgggctacga aattgcgctg
      781 cccaatgaaa aagcggccga tttctggcgc gcgctggtgg aagctggcgt taagccatgt
      841 ggcctgggcg cgcgtgacac gctgcgtctg gaagcgggca tgaatttgta tagccaggag
      901 atggacgaaa ctatttctcc tttagccgcc aatatgggct ggactatcgc ctgggaaccg
      961 gcagatcgtg actttatcgg tcgtgaagct ctggaagcgc agcgtgaaca tggcacagaa
     1021 aaactggttg gtctggtgat gaccgaaaaa ggcgtgctgc gtaatgaact gccggtacgt
     1081 tttactgatg cgcagggcaa ccagcatgaa ggcattatca ccagcggtac tttctcccca
     1141 acgctgggtt atagcattgc gctggcgcgc gtgccggaag gtattggtga aacggcgatt
     1201 gtgcaaattc gcaaccgtga aatgccggtt aaagtgacga aacctgtttt tgtgcgtaac
     1261 ggcaaagccg tcgcgtgatt tacttttttg gagattgatt gatgagcaac gtaccagcag
     1321 aactgaaata cagcaaagaa cacgaatggt tgcgtaaaga agccgacggc acttacaccg
     1381 ttggcatcac cgaacacgct caggagctgt taggcgatat ggtgtttgtt gacctgccgg
     1441 aagtgggcgc aacggttagc gcgggcgatg actgcgcggt tgccgaatcg gtaaaagcgg
     1501 cgtcagatat ttatgcgcca gtaagcggtg aaatcgtggc ggtaaacgat gcactgagcg
     1561 attctccgga actggtcaac agcgaaccgt atgcaggcgg ctggattttc aaaatcaaag
     1621 ccagcgatga aagcgaactg gaatcactgc tggatgcaac cgcatacgaa gcattgttag
     1681 aagacgagta acggctttat tcctcttctg cgggagagga tcagggtgag gaacatttac
     1741 gcctcaccct cactctcttc gtaaggagag aggttcacaa ttcactgcac gtttcaggaa
     1801 ccatcgctca tgacacagac gttaagccag cttgaaaaca gcggcgcttt tattgaacgc
     1861 catatcggac cggacgccgc gcaacagcaa gaaatgctga atgccgttgg cgcacaatcg
     1921 ttaaacgcgc tgaccggcca gattgtgccg aaagatattc agcttgcgac tccgccgcag
     1981 gttggcgcac cggcgaccga atacgccgca ctggcagaac tcaaggctat tgccagtcgc
     2041 aataaacgct tcacgtctta catcggcatg ggttacaccg ccgtgcagct accgccggtt
     2101 atcctgcgta acatgctgga aaatccgggc tggtataccg cgtatactcc gtatcaacct
     2161 gaagtctccc agggccgcct tgaagcactg ctcaacttcc agcaggtaac gctggattta
     2221 accgggctgg atatggcctc tgcttctctt cttgacgaag ccaccgctgc cgccgaagca
     2281 atggcgatgg cgaaacgcgt cagcaaactg aaaaatgcca accgcttctt tgttgcttct
     2341 gacgttcatc cgcaaacgct ggatgtggtc cgtactcgtg ccgaaacctt tggttttgaa
     2401 gtgattgtcg atgatgcgca aaaagtgctc gaccatcagg acgtcttcgg cgcgctgtta
     2461 cagcaggtcg gcactactgg tgaaattcac gactacactg cgcttattag cgaactgaaa
     2521 tcacgcaaaa ttgtggtcag cgttgccgcc gatattatgg cgctggtgct gttaactgcg
     2581 ccaggtaaac agggcgcgga tattgttttt ggttcggcgc aacgctttgg cgtaccgatg
     2641 ggctacggtg gcccacacgc ggcattcttt gcggcgaaag acgaatacaa acgctcaatg
     2701 ccgggccgta ttatcggtgt atcgaaagat gcagctggca acaccgctct gcgcatggcg
     2761 atgcagactc gtgagcaaca tattcgtcgt gaaaaagcga actccaacat ttgtacatcc
     2821 caggtcttgc tggcaaacat tgccagtctg tatgccgttt accacggtcc ggttggcctg
     2881 aaacgtatcg ctaaccgcat ccaccgtctg accgatatcc tggcggcggg cctgcaacaa
     2941 aaaggtctga agctgcgcca tgcgcactat ttcgatactt tgtgtgtgga agtggccgac
     3001 aaagcgggcg tgctggcgcg tgccgaagcg gctgaaatca acctgcgtag cgatattctg
     3061 aacgcggttg ggatcaccct tgatgaaacc actacgcgcg aaaacgtgat gcagcttttc
     3121 agcgtgctgt tgggcgataa ccacgggctg gacatcgaca cactggacaa agacgtggct
     3181 cacgacagtc gctctattca ggctgcgatg ctgcgcgacg acgaaatcct cacccatccg
     3241 gtgtttaatc gctaccacag cgaaaccgaa atgatgcgct atatgcactc gctggagcgt
     3301 aaagatctgg cgctgaatca ggcgatgatc ccgctgggtt cctgcaccat gaaactgaac
     3361 gccgccgccg agatgatccc gatcacctgg ccggaatttg ccgaactgca cccgttctgc
     3421 ccgccggaac aggctgaagg ttatcagcag atgatcgcgc agctggctga gtggctggtg
     3481 aaactgaccg gttacgacgc cgtttgtatg cagccgaact ctggcgcaca gggcgaatac
     3541 gcgggcctgc tggcgattcg tcattatcat gaaagccgca acgaagggca tcgcgatatc
     3601 tgcctgatcc cggcttctgc gcacggcact aacccggctt ccgcgcatat ggcaggaatg
     3661 caggtggtgg ttgtggcgtg tgataaaaac ggcaacatcg atctgactga tctgcgcgcg
     3721 aaagccgaac aggcgggcga taacctctcc tgtatcatgg tgacctatcc ttccacccac
     3781 ggcgtgtatg aagaaacgat ccgtgaagtg tgtgaagtcg tgcatcagtt cggcggtcag
     3841 gtttaccttg atggcgcgaa catgaacgct caggttggca tcacctcgcc gggctttatt
     3901 ggtgcggacg tttcccacct taacctgcat aaaactttct gcattccgca tggcggtggt
     3961 ggtccgggta tgggaccgat cggtgtgaaa gcgcatctgg ctccgtttgt accgggtcat
     4021 agcgtggtgc aaatcgaagg catgttaatc cgtcagggcg cggtttctgc ggcaccgttc
     4081 ggtagtgcct ctatcctgcc aatcagctgg atgtacatcc gcatgatggg cgcagaaggg
     4141 ctgaaaaaag caagccaggt ggcaattctt aacgccaact atattgccag ccgtctgcag
     4201 gatgccttcc cggtgctgta taccggtcgc gacggtcgcg tggcgcacga atgtattctc
     4261 gatattcgcc cgctgaaaga agaaaccggc atcagcgagc tggatattgc caagcgcctg
     4321 atcgactacg gtttccacgc gccgaccatg tcgttcccgg tggcgggtac gctgatggtt
     4381 gaaccgactg aatctgaaag caaagtggaa ctggatcgct ttatcgatgc gatgctggct
     4441 atccgcgcag aaattgacca ggtgaaagcc ggtgtctggc cgctggaaga taacccgctg
     4501 gtgaacgcgc cgcacattca gagcgaactg gtcgccgagt gggcgcatcc gtacagtcgt
     4561 gaagttgcag tattcccggc aggtgtggca gacaaatact ggccgaccgt gaaacgtctg
     4621 gatgatgttt acggcgaccg taacctgttc tgctcctgcg taccgattag cgaataccag
     4681 taatttactg attcgactat cttctaaagg cgcttcggcg cctttttcgc ttgttaataa
     4741 agtgcgcttt gtgtgtttcg gttgaggcgt aatataaaat attgcgagct caatatatta
     4801 aaagagtgcc aggcctgatg agcggagcac atcaggcaca tcggctaatt acaaattctc
     4861 gccgttgctg gcaatcacct ctttgtacca gttaaagctc ttcttacgtg aacgcgacat
     4921 atcgccagta ccgtcgtcat gtttattcac atagataaag ccgtagcgtt tgctgtactg
     4981 cccggtagtg aacgacacgc agtcgatgca gccccacggt gtgtagccca tcagatccac
     5041 gccatcgtaa gtcaccgctt ttttcatctc ttcaatatgg gcgcgcaggt agtcaatgcg
     5101 gtaatcgtca ttgatgctgc catcatcttc cactttgtcg taagcgccaa aaccgttttc
     5161 gacaataaac agcggcttct ggtaacgctc atacagctcg caaagcgcgt aacgcagacc
     5221 aaccggatca atctgccagc cccagtcaga cgctttaaca tacgggttcg gtacgctgcc
     5281 ttcaaaacca gagatcgcat cgccggtgcc gccttcggcc ttcaccgcgt tggtcatgta
     5341 atagctgaag ccgagataat cgcaggtgcc ttcgcgcagt acatccagat cgccgtcttc
     5401 cattttgatg ttaaatccgc ggcgttccca ctcgttcaac acataggacg ggtaatagcc
     5461 acgcaactgc acatcggtaa agacgtaacg ttcgcgcatc gacttctggg caaacatcac
     5521 atcgtccggg ttacaggagt agggatagag cggcaccatc gccagcatac aaccgacttt
     5581 catctccggg ttgatgcgat gcgcggcttt caccgccagg gcgctggcga caaactggtg
     5641 atgcagcacc tgatacatgg tctcttccgg gttttcatgt tcggtataca ccacgccgga
     5701 gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt cgttgaaggt
     5761 catccagtat ttgactttgt gcttatagcg ttcgaatacc acttccgcga aacggacaaa
     5821 gaaatcaacc actttacggt tggtccagct accatattgc tgcaccagat gcagcggcat
     5881 ttcaaagtgg gagagggtga tcaccggttc gatgttgtat ttcagcagtt catcgatcat
     5941 atcatcgtag aacttcagcc cttcttcgtt tggctgagct tcatcgcctt ttggaaaaat
     6001 gcgcgtccag gcgatggaag tacgaaaaca tttgaagccc atttcggcaa atagcttgat
     6061 gtcctccttg tagtgaccat aaaaatcaac ggcttcatgg tttggatagt atttgccagg
     6121 cacaacttct ttggtgattt cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca
     6181 aatgctcggc cctttgccgc ctttgttcca gccgccttcg acctgatgag cggcaactgc
     6241 gccgccccat aagaaatctt tcggtaaggt gagttttttc actatcatag gctccagatg
     6301 agtcaattct tacaggaaat attaccatgc agccaaacga catcactttt tttcaacgtt
     6361 tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag tctgaatcgc
     6421 acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga agatgacggt tatttttgca
     6481 cgattgaagt caccgcaacc tcaaccgtaa cgctggatac gctgacggaa aaacatgcag
     6541 agcaggaaaa tatgaccctg actgaactga aaaaggtcat tgccgacatc tatcccgacc
     6601 agacacagtt ttatgtgatt gaatttaaat gtctttaaat tgaactcacg cctgttagtt
     6661 gaaatttcgc atttaatttc atgatttttc ggagattaat ctcaaatcag aatttatttt
     6721 agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata catgtgcgtg
     6781 tacgtttttc cggagtaagt tatggttcag aagcccctca ttaagcaggg atattcgctg
     6841 gcagaggaaa ttgccaacag cgtcagtcac ggcattgggc tggtgtttgg tatcgttggg
     6901 ctggtgttgc tactggttca ggcggtggat ctcaatgcca gcgccacggc gataaccagt
     6961 tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct ctatcacgcc
     7021 attcctcatc aacgggcaaa aatgtggctg aagaaatttg accactgcgc tatttatctg
     7081 ttgattgccg gaacctacac gccgtttttg ctggtggggc tggattctcc gttagcgcgc
     7141 gggttgatga ttgttatctg gagcctggca ttgctgggta ttctgtttaa actgaccatc
     7201 gcgcaccgat tcaaaatttt atctctggtg acctatctgg cgatgggctg gctgtcgctg
     7261 gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt actggcggta
     7321 ggcggtgtgg tttattcgct cggggtgatt ttctacgtct gcaagcgcat tccatacaat
     7381 catgccatct ggcacggctt tgtgctcggc ggtagtgtgt gccactttct ggcgatctat
     7441 ttgtatattg ggcaggtgta attggcgtgc ctttgtaggc ctgaaaagac acaccagtgt
     7501 cgcatcaggc gataaatgcc tgatgcgata cagagattta ctcttccagc gaatatggca
     7561 gtggctcaat acgcaatgta ttcgcatcgt cacgcacgcg gaagatgctg tccggttcca
     7621 tatcgttatt catgacaacc tgcaccacaa cctgaccatc ttccagtttg accgcagcca
     7681 gcaccgtacc ggtacgacgc cagttctcgc ccattttcag ctctaagtct tcaccagctt
     7741 ccggcagtcg gctggcgcta cccgtcagca accagagcgc gcgtttgttg gcaccacgga
     7801 attttgctcg cgccaccatc tcttgtccgg tatagcagcc tttcttaaag ctgatgccgc
     7861 ccagcgcctg gaggttggtc gcctggggga taaactgccc gctgttggcg gcatcaatca
     7921 ccggaaaacc cgcttcaatg tttaatgcca gccactgttg gctattgttc agttccgctt
     7981 caccgcgcag tttatcggtc agcatattgg cagtagcttc atcggttacg atcaggaaac
     8041 gttctgccgg gtgttcaaac catagcaacg tggtcgcgcc ttctttgact acctgttttt
     8101 ctctcgaagg tagttcacta aagagatttg ccaacgcggt gcgcgcctga aaaccggcaa
     8161 caccaagcag cacacgctcg tcgtctggcg cgatggtcac tttagagaac accgcatatt
     8221 ttttcagttc agtcagctgc ggttcacgca cgctgcgccg ttcaatccat gcaaagccat
     8281 cgccgtcgcg gaacagacgt aaattgctcc acattttacc tttggcgtcg caatgggcgg
     8341 cgagcaggtg ctgatcttca gtcatctggc tgacatctgc tgtcacctgg ccttgcatat
     8401 atttttcgct gtccgcgcca gtaatagtgg caagcgccca gtcatcaagc gtcatcagcg
     8461 tcagtggcaa acgggcagaa gccgtaggct gacggggagg aaaaggtgta aaagccataa
     8521 tagagtcctg agttgcttaa cggcgtatta atcactaatg gtaaaagagc tatgaggcaa
     8581 tgcaagtgct ttcactgcgc gatctgtgcc ttctgcgagg cgacttccaa aaaaatgaag
     8641 cccttgaatt gttgtgaatt aatttcgcga tcgcgattgt gtttactgat attcccgtaa
     8701 aagaagtcga aaaacacgtt acaatagccg ggtactggat tttcgtgaga caggaagaag
     8761 atggacatta acaacaaagc ccgcattcat tgggcatgcc gccgtggtat gcgcgaactc
     8821 gatatttcaa tcatgccgtt tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa
     8881 cgcaccttta ttcgtctgct ggaatgtgac gatccggacc tgtttaactg gctgatgaat
     8941 cacggtaaac cagccgatgc agaactggaa atgatggtcc gactcatcca gacacggaac
     9001 cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc tggcgcgcac agtggctttc
     9061 cttgctgatt catgggctgg ttgccgctgt tattttactc attccctggc cgctcagtta
     9121 caccccgtta tggatggtgt tactttcgct ggtggtgttt gattgcgttc gcagccagcg
     9181 gcgcattaat gctcgccagg gggaaattcg cttgttgatg gacgggcgtt tgcgttggca
     9241 agggcaggag tggagcatcg tcaaagcgcc gtggatgatt aagagcggca tgatgctgcg
     9301 tttacgttct gacagcggaa aacggcaaca tttatggctg gcagctgaca gcatggacga
     9361 agccgaatgg cgggatttac ggcggatatt gttgcaacaa gagacgcaaa gataaagatg
     9421 acggcaaagc atatgtgcta agccgtcagt gacatcaggc gtaatgctct gccatttcgt
     9481 tgaggatttg ctcgcaccag ctttgaatac gctcgtcgct aaggtcatac tggttagttt
     9541 catccagcgc cagacccacg aacagttgcc cgtcagcaat caccggtttc ggactggtaa
     9601 attcatatcc ttccgttggc cagtagccga cgaacttcac gcctttggtc gagagtttgt
     9661 catgcagcat accgagcgca tcgaggaacc actcgccgta tcccagttga tcgccaagcc
     9721 catacagcgc aacaatttta ccttcgaggt tcaggtcgtc gagctgatcc cagacggctt
     9781 cccagtcttc ctggatttcg ccaaaatccc aggtcgggat acccagaatg agcacatcgt
     9841 actgctccat taatttcggg gagtcgtcct tgaggttatg taaggtcacc agttctgggc
     9901 cgataatatc gcggattttt tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa
     9961 aaagacccat attcataacg taagcttctc gattctgctg tggcttttgc ggctagtgta
    10021 tcagaatcgc tttagtcacg gcacaataag gcataatgga tcaaatgagt gaccataagg
    10081 ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt cttgatgctc tgtggctgga
    10141 aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga
    10201 gtggttgcat caccgcgggt tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt
    10261 actggcagaa cggctggagg gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc
    10321 agtgcgccga ttgttccagt atctttatcg cgaaaagttt cgtgaagatg atcccagtgc
    10381 gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt
    10441 cgaacgttta ttacaggcac cattaattga tcagccactg gagctacgcg ataaagccat
    10501 gcttgaagtg ttgtatgcta ccggactgcg tgtctctgaa ctggtcgggc tgacaatgag
    10561 tgatatcagc ctgcgtcagg gcgtggtgcg ggtcattggt aaaggcaaca aagagcgcct
    10621 ggtgccgtta ggtgaagagg cggtttactg gctggaaacc tatctggaac atgggcgtcc
    10681 gtggctgttg aatggtgtgt caattgacgt gttgtttccc agccagcgtg cgcagcagat
    10741 gacgcgacag accttctggc accgcattaa acattatgcc gtgctggcgg gtatcgacag
    10801 cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc actcacttat taaatcatgg
    10861 tgcggattta cgcgtggtgc agatgctact gggccacagc gatctctcca ccacgcaaat
    10921 ttatacgcat gtcgctaccg agcgtctgcg acaacttcat caacagcatc acccgcgggc
    10981 gtgatgtctg aaaagaacag gaaaatttat gaagaaaggt tttatgttat ttaccttgtt
    11041 ggcagcgttt tcaggctttg ttcaggctga tgacgcggca attcaacaaa cgttagccaa
    11101 aatgggcatc aaaagcagcg atattcagcc cgcgcccgta gctggcatga agacagttct
    11161 gactaacagc ggtgtgttgt acatcaccga tgatggtaaa catatcattc aggggccaat
    11221 gtatgacgtt agtggcacgg ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt
    11281 gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg caggaaaaac acgtcatcac
    11341 cgtgtttact gatattacct gtggttactg ccacaaactg catgagcaaa tggcagacta
    11401 taacgcgctg gggatcaccg tgcgttatct tgctttcccg cgccaggggc tggacagcga
    11461 tgcagagaaa gaaatgaaag ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga
    11521 tgtgatggca ggtaaaagcg tcgcaccagc cagctgcgac gtagatattg ccgaccatta
    11581 cgcacttggc gtccagcttg gcgttagcgg tactccggca gttgtgctga gcaatggcac
    11641 acttgttccg ggttaccagc cgccgaaaga catgaaagaa ttcctcgacg aacaccaaaa
    11701 aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc
    11761 gatgaaacgg ccgacttgcc cgctgaattg cctcccttgc tgcgccgttt atatgccagc
    11821 cggggtgtgc gtagcgcgca ggaactggaa cgcagtgtta aaggcatgct gccctggcag
    11881 caactgagcg gcgtcgaaaa ggccgttgag atcctttaca acgcttttcg cgaaggaacg
    11941 cggattattg tggtcggtga tttcgacgcc gacggtgcca ccagcacggc tctaagcgtg
    12001 ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggttccaaa ccgtttcgaa
    12061 gacggttacg gcttaagccc ggaagtagtc gatcaggctc atgcccgtgg cgcgcagtta
    12121 attgtcacgg tggataacgg tatttcctcc catgcggggg ttgaacacgc tcgttcgttg
    12181 ggcattccgg ttattgttac cgatcaccat ttgccaggcg acacattacc cgcagcggaa
    12241 gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact ggcgggcgtt
    12301 ggcgtggcgt tttatctgat gctggcgctg cgcacctttt tgcgcgatca gggctggttt
    12361 gatgagcgcg gcatcgcaat tcctaatctg gcagaactgc tggatctggt agcgttgggg
    12421 acagtggcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg gcaggggatg
    12481 agtcgcatcc gtgccggaaa gtgccgtccg gggattaaag cgttgctgga agtggccaat
    12541 cgcgatccac aaaaactcgc cgccagcgat ttaggttttg cgctggggcc acgtctcaat
    12601 gctgccgggc gactggacga tatgtccgtt ggcgtggcgc tgttgctgtg cgacaacatc
    12661 ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa accagacgcg aaaagagatc
    12721 gaacagggaa tgcaagttga agccctgacc ttgtgcgaga aactggagcg cagccgcgac
    12781 acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt tgtcggtatt
    12841 ctggcttcgc gcatcaaaga gcgttttcat cgtccggtta tcgcctttgc gccagctggt
    12901 gatggtacgc tgaaaggttc cggtcgctcc attcaggggc tgcatatgcg tgatgcgctg
    12961 gagcggctag acacgcttta ccccggcatg atgctcaagt ttggcggtca tgcgatggcg
    13021 gcgggtttgt cgctggaaga ggagaaattc gaactctttc aacaacggtt tggcgaactg
    13081 gttactgagt ggctggaccc ttcgctattg caaggcgaag tggtgtcgga cggtccgtta
    13141 agtccggccg aaatgaccat ggaagtggcg cagctgttgc gcgatgctgg cccgtggggg
    13201 cagatgttcc cggagccgct gtttgatggt catttccgtc tgctgcaaca gcggctggtg
    13261 ggcgaacgtc atttgaaagt catggtcgaa ccggtcggcg gcggtccact gctggacggg
    13321 attgctttta atgtcgatac cgccctctgg ccggataacg gcgtgcgcga agtgcaactg
    13381 gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac
    13441 aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt gggtacaatc
    13501 ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat gtttgaaatt
    13561 aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct tagggggtat
    13621 ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc tggaacagcc
    13681 ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga
    13741 agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt ctggtctgct
    13801 ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg ctgaactcga
    13861 cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg gcgaatatga
    13921 cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag cacaggactg
    13981 ggcgagcatg cttgagcgta tgtatctgcg ctgggctgaa tcgcgtggtt tcaaaactga
    14041 aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacga tcaaaatctc
    14101 cggcgattac gcttatggct ggctgcgtac agaaaccggc gttcaccgcc tggtgcgtaa
    14161 gagcccgttt gactccggcg gtcgtcgcca cacgtcgttc agctccgcgt ttgtttaccc
    14221 ggaagttgat gatgatatcg atatcgaaat caatccagcg gatctgcgca tcgacgttta
    14281 tcgcgcatcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac
    14341 tcacatccca accgggatcg tgacccaatg ccagaacgac cgttcccagc acaagaacaa
    14401 agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa
    14461 tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca gccagattcg
    14521 ttcttatgtc cttgatgact cccgcattaa agatctgcgt accggggtag aaactcgcaa
    14581 cacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg
    14641 gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct
    14701 taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgtgagc aggggattgc
    14761 cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg
    14821 taaagagaac gaagaactgg aagcgctgaa catcgaagtc gccgttgctg gccgcatgat
    14881 gacccgtcgt attatgggta aagcgtcttt cgttaccctg caggacgttg gcggtcgcat
    14941 tcagttgtac gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa
    15001 atgggacctc ggcgatatcc tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga
    15061 actgtctatc cactgtaccg agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga
    15121 taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatt tggatctcat
    15181 ctctaacgat gaatcccgca acacctttaa agtgcgctcg cagatcctct ctggtattcg
    15241 ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc aggtgatccc
    15301 tggcggtgcc gctgcgcgtc cgtttatcac tcaccataac gcgctggatc tcgacatgta
    15361 cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt
    15421 attcgaaatc aaccgtaact tccgtaacga aggtatttcc gtacgtcata acccagagtt
    15481 caccatgatg gaactctata tggcttacgc agattacaaa gatctgatcg agctgaccga
    15541 atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga cctacggcga
    15601 cgtaacgctg gacttcggta aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa
    15661 atatcgcccg gaaaccgaca tggcggatct ggacaacttc gactctgcga aagcgattgc
    15721 tgaatctatc ggcatccacg ttgaaaagag ctggggtctg ggccgtatcg ttaccgagat
    15781 cttcgaagaa gtggcagaag ctcatctgat ccagccgacc ttcattactg aatatccggc
    15841 agaagtttct cctctggcgc gtcgtaacga cgttaacccg gaaatcacag accgctttga
    15901 gttcttcatt ggtgggcgtg aaatcggtaa cggctttagc gaactgaatg acgcggaaga
    15961 tcaggcgcag cgcttcctgg atcaggttgc cgcgaaagat gcaggtgacg acgaagcgat
    16021 gttctacgac gaagattatg tcaccgcact ggaacatggc ttaccgccga cagccggtct
    16081 gggaattggt atcgaccgta tggtgatgct gttcaccaac agccatacca tccgcgacgt
    16141 tattctgttc ccggcgatgc gtccggtaaa ataagcatta cgttatgctc acaaccccgg
    16201 caaccgtcgg ggttttttat ttaagttggg taaatgcaga taatcgtttt ctggcttcgc
    16261 gatttgtcgc ctgcatcacc atccacggac tgaacgccca cggcgtagca tcaataccgc
    16321 gtaaaaccgc tgctaaatca caccattgat aatccatcac ttcatcatcg ttgatctgta
    16381 acgcactggt cgtgcgtgcg gcaaataccg gacacacttc attttccaca atgccattcg
    16441 gatcggtggc gcggtagcga aagtcaggat agatagattc aggaggcgta atttctacgc
    16501 caagctcata acggcaacgg cggatcaccg cctcttcgtt gctttctccc agttgtgggt
    16561 gcccacaaac cgagttagtc cacacgccag gccatgcttt tttgctaagg gcgcggcggg
    16621 taactaataa ttgccctttg gcattaaaca gccaactgga aaacgcgaga tgtaagaggg
    16681 tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc tgtgcattca
    16741 ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca ctcatcatta
    16801 tagataatag aagagtgaaa tgattaacca agtgagcgtt tatcgacaac cgcccgtttt
    16861 gagcggatgt cgacaggtaa aaaccattta aaaaagcagc atcatatatt tgaagcgact
    16921 ttcaatggcc attgcaacac aatcagaaat gacaggaata gagagagtaa aaagcaacag
    16981 cagactgaat atagcgcgca ttaaaagatt catattattc tccatgctca tttttcttca
    17041 gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta tcagagacgg
    17101 attccttcac caggtcagca tgatgctggt agccattaaa gaagacgttc agcacaacgg
    17161 cgctaagggt tgcgagcata atgccgctat gcagcaacgg ttgcagtacg gccggtaact
    17221 tagaaaagaa atcgtgagag agcgttggcg tcatgccaac accgagactg atcgccacaa
    17281 tatagaggtt gtaacggtta gtggtgtagt tacagcgcga cagaattcga atccctgtcg
    17341 ccagtaccat gccgaacatc accagccccg cgccgcccag cacaaattgc ggaatggagg
    17401 cgaccagcac cgccattttg ggcaccatgc caaacaggat taatataatt cccgaagaaa
    17461 tacacaccca gcggctgtgg acgcgcgtca cgctgaccag cccgacgttc tgcgaaaatg
    17521 acgtatgtgg gaagctgtta aacgtgcctc caatcattgt cccgacccca tctacgcgta
    17581 acccacgaat aatgtcctgg gaagagagtt tgcgaccgac tatttcaccc agtgccagga
    17641 acatccccat tgactcaata aatacgatga tgagtactgc ggtcatggtc aaaatggaaa
    17701 cgggatcgaa aatcggcata ccaaatgaca tcggcgtgac aatcgcaaac catgaagcat
    17761 catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag ccaaatacaa
    17821 tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta atgagcaaga
    17881 taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat tgcggattcc
    17941 ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagcccg atagaagtaa
    18001 tcaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata agtggcgcta
    18061 ataatgtggt gataaaaccc gcggcgatag tggcaccgaa tatccccagc aggccgatat
    18121 ccgggttcat accaatggct atcattggtg ttacagcggc gaaggtcacc gacataatca
    18181 ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat gtgacgatcc
    18241 cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgat aggccgagtc
    18301 ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg acgtgttgca
    18361 gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca gttgggcggt
    18421 cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt tctcctttcg
    18481 cccgccagag taaaaagcaa ccgtttatgt tcatgaaact gtctaaacac atgctcgtgg
    18541 agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc aatcacataa
    18601 tcgtggttaa accacgaaaa tgagaagaga acctgacaat ggaattatct gtaattttct
    18661 catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa tcgtttttat
    18721 ctggatagca ttcttttgat cggcaatgtt gaaataatga aagcggcata gattgagggg
    18781 caataatttg aactcagttg caaaattata attaaaacgg tcatgctacc taatatatgc
    18841 ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag ggatgcaaat
    18901 gaataagttt atcgctgctg aagctgcgga atgtataggc tgccatgctt gtgaaattgc
    18961 ctgtgcggtg gcacataatc aagaaaactg gccgctgagt cacagtgact ttcgaccgcg
    19021 tatccacgtt gtagggaaag gccaggctgc gaatccggtg gcctgccatc actgcaacaa
    19081 tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg atagcgtaca
    19141 actggacgag caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc cctttggcgt
    19201 cgttgagatg gtcgatacga ttgcacagaa atgcgacctt tgtaaccagc gcagttccgg
    19261 cacgcaagcc tgtattgacg tctgcccaac acaggcgtta cggctgatgg acgataaagg
    19321 gttgcagcag ataaaggtgg cccgccagcg caaaacggca gcaggaaaag cgtcatcaga
    19381 cgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag gcgcagataa
    19441 aatttcagcg agtgaacgga aaacccactt tggcgaaatc tattgcgggc tggatccaca
    19501 acaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta actgcaactg
    19561 gcattgtccg ctgcataacg ctattccgga ttacatccgt ctggtacagg aaggaaagat
    19621 tattgaagcg gcagaacttt gccaccagac cagttcctta cccgaaatct gcggcagggt
    19681 atgtccacag gaccgtcttt gtgaaggtgc atgtactttg aaagatcact ctggcgcagt
    19741 ctctatcggt aatctggaac gctacatcac cgatactgcg ctggcgatgg gctggcgtcc
    19801 cgatgtcagc aaagttgttc cccgtagcga aaaagtggcg gtgattggcg ctggacctgc
    19861 aggattaggg tgtgctgata ttctggcgcg cgcgggagtt caggttgatg tctttgatcg
    19921 ccatccagaa attggcggta tgctgacttt tggcattcct cctttcaaac tcgataaaac
    19981 ggtattaagc cagcggcgag agatattcac cgcaatggga atcgatttcc atctcaactg
    20041 tgaaattggc cgcgatatct ctttcaacga attaacggcg gaatatgatg ctgttttcct
    20101 cggcgtgggg acttacggga tgatgcgagc agatctgccg catgaagatg cgcctggtgt
    20161 cattcaggcg ttaccgttcc tgaccgccca tacccgccag ctcatgggat tgccggagtc
    20221 tgcagagtat ccgctgacgg atgtggaagg taagcgagtt gtggtattgg gcggtggcga
    20281 tacgacaatg gattgtttgc ggacttccat ccgccttaat gctgccagcg tgacctgtgc
    20341 gtatcgtcgt gatgaagtca gtatgccggg ttcgcgcaaa gaggtggtca atgcccgcga
    20401 ggaaggtgtt gagtttcagt tcaatgttca gccgcaatat atcgcttgtg atgaagatgg
    20461 acgcttaacg gcggtgggcc tgattcgtac cgctatgggt gagccggggc cggatggccg
    20521 ccgtcgtcct cgtccggttg cgggttcaga gtttgaattg cccgccgatg ttctcattat
    20581 ggcctttggt ttccaggcac ataccatgcc gtggttgcag ggcagcggaa ttaaactcga
    20641 taaatggggc ctgattcaaa ccggcgacgt cgggtattta cccacccaga cgcatctgaa
    20701 aaaagtcttt gctggtggtg atgcagttca tggcgcagat ctggttgtca ctgcaatggc
    20761 cgcaggaagg caggcggcgc gcgatatgtt aactctgttt gatacgaagg catcgtgatg
    20821 aaatcgttaa ttatcgttaa tccggctgac tgtattggct gccgtacttg tgaagtggcc
    20881 tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg atatcttttt accccggctg
    20941 aaggtacaac ggctggatag catcagtgcg ccggtgatgt gccatcagtg tgaaaacgcc
    21001 ccttgtgttg gcgcttgtcc cgtgggggcg ctgacgatgg gcgagcaggt ggtgcaggca
    21061 aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt tggaatgatc
    21121 accattcagt cattgccggg ggatacccgg caacaaatcg tgaaatgcga tctctgtgaa
    21181 cagcgggaag aggggccagc ctgcgttgaa tcttgcccga cacaggcgct gcagttgctg
    21241 actgaaagag aactcaggcg agtccgccag cagcgtattg ttgccagcag tgagaatcct
    21301 ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct attagattgc ccaaccaccc
    21361 gcgtaaaacg cgaccagtgc ggcagtaatg atcaccgtac caatattcag cttttgccat
    21421 tcgcgcgcaa agacgcgacc caccaccagt gttacaaagc ccagcataat accggtaacg
    21481 atattacaag tcagaacgat gaacacggca cacaccaggc cagccattgc gtcaataaaa
    21541 tcgttgaaat ccagcttcga gacgttgctt aacatcagca aacccacgta catcagtgcg
    21601 ggagctgtgg cataaccggg gatcagaaac gataacggtg ataaaaacag aatcatcagg
    21661 aacaacacac caaccacggt tgcggttaaa ccggttttcc ccccggcggc ggttcctgcc
    21721 gctgattcga tataaaccgc cgcgggcgct gcgcccacca ggccggagaa tattgaactt
    21781 actgagtcac tggtcagggc tttgccgccg ttgatgatct ggttgtcttt atccaacaaa
    21841 ttcgcctgac cggcgacggc acggatggtg ccagtggcgt cgaacactgc ggtcatcacc
    21901 aatgccagta cactcggaag tacagttggc tggagtgcgc ccataatatc gaggctgaaa
    21961 atcagagact taccatcttc gccagtcagg cttggcatcg ccaccagacc gtggtacttc
    22021 accgctgggt caaagattaa gccgatgatc gaaattgcaa taatcaccaa caagatcccg
    22081 ccgggtacgc ggcacttttc cagaccgaag atgaccgcca gccccagtaa gctcatcatc
    22141 accgggaagg aggtaaaggc accgagcgcc actggcaagc cttcaatcgg gttttttata
    22201 accattccca caccgttagc agcaatcagc agcagaaaca ggccgatacc gatacctgta
    22261 ccgtgagcga tccccatcgg caaattacgt aagatccagg tacgcacacc ggttacggaa
    22321 atagcggtga agatgacgcc catcagaaag actgcgccca gtgcgacagg aacgctaatt
    22381 tgttgcccga gtaccagact gaatgcggta aacgccgtca aggaaatagc gcaaccaatc
    22441 gccattggca aattggccca taaccccatc agcaacgagc cgaagcccgc gaccagacag
    22501 gtggcaacaa acacagctgc gggaggaaaa cctgctttgc ccagcattcc cggaacgacg
    22561 ataacggaat aaaccatggc cagaaaggtc gttaagccag ccagtacttc ctgacgaacg
    22621 gtactgccac gagcggtaat tttaaaataa ttatccagcg cgccctgtgg ctttggtgcg
    22681 tccggtgttt gtaggatgtc tccagacata atgatgtcct cacgaggatg ttaaagtttt
    22741 attattagtt gcgttcgtac accagacgac catcaacgta ggtgcggtag atcgaacggt
    22801 catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa agaaacagag ttgtcatagc
    22861 gcagctgctg tagcggagtg gcagtgggtt ccatcaccac gaaatcagcc tctttgccag
    22921 gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg agcgtggcca
    22981 ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa ttgcaatacc ttgtaggctt
    23041 cgttcagcgt ttgcagcatg ttaaaagttg ttccggcacc gatatccgtt cccatgccca
    23101 ctttgacttt cttctgccat gcttttttca agttgaataa gccgctgccg aggtaaaggt
    23161 tggaggtcgg acagaaagca atgctggatt tggtttcgct gagacgatcc cactcttttt
    23221 cttcgagatg gacgcagtga gcaaagacgc agtttttacc ggtcaggccg tactgatggt
    23281 aaacatcaag ataaccatca tggtcaggat aaagcgattt cacccaggca atttcatctt
    23341 tgttttcaca gagatgggta tgtacccatg tatccggata ttcttctttc aggcgttgcg
    23401 ccatcgccat ctgttcagga gatgaggtag gggcgaagcg tggcgtaatc gcatacaaca
    23461 gacgaccatt tttgtgccag cgttcaatca gttctttgct ttggtgatag ctgctttcgg
    23521 cagtgtcgag cagataatcc ggtgcgtttc ggtccatcat cactttaccg gcaatcatac
    23581 gcatattgat atgactggcg gcttcaaaca gcgcatcaac ggattgtgga tgaacagtgc
    23641 caaacaccag cgccgtggtg gttccgttac gtaaaagctg cttgatgaag aacgccgaca
    23701 tctcgcgggc gtactctaaa tcctcataac gacgttcagt agggaaggtg tgtttattca
    23761 accactccag caattgttca ccataggccc ccaccatttc actttgcgga taatggatat
    23821 gtgtatcgat aaagcccggt actatcagtt tgccgcgata gtcgcgcacg cgaatggtgt
    23881 caggaatttg atgctttccg tcttcccatt cgccaaacca ttccactttt ccctgtttaa
    23941 tgagtaataa accatcctca ataaaccgca gcgcagaggc aatttcttcc gggttatcga
    24001 ccgtacgggt gacatcaata aaactgcctc gtaccgcttt taacgtgtgt tctcctgaca
    24061 tcattgactc ctttactgtt aatccatctc ttccgctgag gtaataccag gcagaacgtt
    24121 ttcctgtcgg tagccaccag ggaggataat atttaataaa atcgcagtta acccgccagc
    24181 acaaataggg ttttcaacta atacataaat agaggctggc aatattttaa aaatttcggg
    24241 atcgtaggag ataccaagcc ctaaacctaa agaagtggca acaataagcg tttcacggcg
    24301 ctttaaaccg ttggtgatga tgatgcgaat ccctgcgatg gcaatcatgg aaaacatcaa
    24361 cgtcattgcg cctcccagaa ctgctgaggg aatggtcgtg aagaagccgc caatcatcgg
    24421 aaataagccg aggataacca gcattaccgc gatggttcgc ccgacataac gtgaagcgac
    24481 gccagtcatc tgaataaccc cattattttg cgcaaacgtg gttaatggta atgaaccgac
    24541 agcggaggcg ataacagaaa ccagaccatc tgccagcacg ccgcctttca gtcgggactg
    24601 atactcttcc ccctgaatgg gacggcggga aaccattgcc gtggcggtga tatcgccgac
    24661 agcttccagc acgctaagca gataaatcgt gccaaccacc aggaactgat ggaagctaaa
    24721 actaaagccg tatttgaacg gatgcgggat ggtgattaac ggcaaattgc gcatactgct
    24781 gaaatccacc atgcccaggc ataacgatgc gatatagccg acacatagcc caatggcgat
    24841 ccctcccatg cgtagcaacg gactgcgaca gcagttaaag ccgatcacca caatcaaaac
    24901 caataaacca acgccgagat gttcgtaatt gccgaacgta ccgctgcttt tggctgcaaa
    24961 tcctccacca aaatcaataa tgccgacttt aatcaggctt aagccgatca tcagcaccac
    25021 aatgccgctg acggtaggcg taataacccg acgtaaatag ggcaggataa aagaagagcc
    25081 gacaaccaga aatgcgccaa cgaaggagac gccgaggagc gacgacatga ttaactcttc
    25141 gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa caaatgaaaa
    25201 attgactgac tgaattgaaa gtaaaccaga accgacgatg ccgtagcggt ttacttgtaa
    25261 ccaggtacca ataccagagg cgatcatcgc catagaaaca agataggcag ttgtttcagc
    25321 ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt accatcggaa cgaaaattgc
    25381 caacagatgg gtaatggcac ctacgagagc ctgatgaaag ggagggcgat cctccagttc
    25441 aaatataagg tcagaacctg catggtttat atcagacatc ccttcactcc ttttaaagta
    25501 aatttgatca tctgaattag taaaataaat tcccacgaat ttggggcgca ggacgccttg
    25561 agaacggctt atctcatttt cgcagtcact atttagtcat cgttcaaaca gagaaacagg
    25621 ccgccgaagc ggccatttat tacttatatt ttttccagcg cggtgagtat tttctccggt
    25681 gtgaaatgcc attcgcgtaa ccagatgccg caggcatcgt gaattgcggt agcaatcgcc
    25741 ggagctgcgc catttacacc gatttccgag atcgatttcg ccccgaatgg gccgacttta
    25801 tcgtcgctcg gcaccagcac agcgcggaaa tcacgcggaa tgtcaccaat tttcggtgcg
    25861 ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg cgtcataaat gatctcttcg
    25921 ctcatactgt ggccgatagc acgcagggtg gcaccgtaga tttgtcccaa cgctaactct
    25981 ggattgaccg gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg gatttcaccc
    26041 gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat acgggaaggc gaaatcaggt
    26101 gtgatataac tgccagttcc caccagtgaa ccaaagccgg tgccggtttc gcctttatgg
    26161 gctatctctc cgaaactaac ttcgcctttc ttgccgcgaa cgacgcccgg cgttgctagt
    26221 tgaacatctg ccactggctc acccaatatt tgcgcgccgt ggaacaggat tttctcccgc
    26281 agattttccg ctgccaaacg cgcagcgtta cccgagaagc aagtaccgga cgaggcatat
    26341 gcgcctttat caaacagtgc atgatcggta tcaccagaga taacatgcac gtcctgcggt
    26401 gggcagtgca gcacttctgc tgccagtttg gtcaccacag tatccagacc agtaccaata
    26461 tccgcaccac cggaatgaac gataaaggtg ccatccgatt ccagtttgat catgcagtta
    26521 gcctgatcga tatccgggat acccgatttc tgcatgataa tggcgacgcc gcgaccgatg
    26581 tgccagtcac cattttgcgg ttttggtgaa gaccattgga tcatctcgcg accctgacgc
    26641 aggatctctt ccagtgcgca gctggcggcg gaaggaacgg aggtcggcgc tttaccttca
    26701 ccgattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg ttcgataatt
    26761 tccagttggt cgatctgtaa ctgttcagcc agttccgcta atgccatggt gatagcgaag
    26821 ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt gctgtagtag
    26881 gtggtgacct gaaaatcgac gttatcgcat ggatataacg gcagcgacag cgccggtccg
    26941 ttacacggta cggtgagtga gtggttaccg taaggacccg tgttggcgcg gaaatccatc
    27001 ttcactgccg tcaggcgacc atcttttttc gcgcccagtt tgacggtgac tttcgcgacg
    27061 tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaaaag taccggacgc
    27121 ccggtcacgc aggttgccca ggcgcacact tcttccagca ggatgtcctg tttggaacca
    27181 aaaccgccgc caactcgctc tttaatgaca tgaactttat gctgtttcat gcccacgagg
    27241 cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat caccagacga
    27301 tcgccgtcca tacgggtaaa gcagatatgt gtttcagtcg ggcactgctg tgcctgcgtt
    27361 gaattatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc catatcgcca
    27421 atatgaccat gaatactggc ggcgatattt ttgcgtgggc gagaaccgat cgggaagttg
    27481 atgatcatat gctcgccgcg ctgggctgca tggctgttat catcttccag agtatctggc
    27541 gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc cgccattgcc
    27601 tcgtcaatcg acattaccgg cttaagcact tcatattcaa cgtcgatgag cttcagtgct
    27661 tcgagcgcaa tgtcttcact ttcggctact accgcagcaa cgcgatcgcc gacgtgacgc
    27721 attttcttgc cgaacattcg gcggtcaagc ggtgacggtt ccggtgcgct ctgaccaccc
    27781 ggggtatagt aaatatccgg gcaattcagg tgagtaataa cgtgaacgac gcccggtaag
    27841 gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg gctacgtaac
    27901 attttaatga cgcaggcgtc agccgttacg cgatcttcaa catagcaggg tttcgcctgc
    27961 accattttcg cggcatcagt tttaggataa tgcttgccaa tgacttcaag gtcgtcacgg
    28021 aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac cgccagttca
    28081 atgacctgat agtattgctg ccagccagca tcgcggctaa acagacctga gagtgcgtcg
    28141 tcgatctctt cgcgggtagg tgcgtcgatg cgatcgagaa gatcggtgat aatcagagcc
    28201 gcagctggat cgttataacc agactgcacc acgccaacat caaccatggc ctgttgaacc
    28261 agacttaact cattccattt gcccagagat tctgcggtac gaatatctgc cttctctaac
    28321 tgtgcggcga taagcaagga cgcgttaacg atattaccgt taaagataat tgcgtcagaa
    28381 ccggcaaacc caaagccatc atcactgttg cgtacagagt gcattcccat gttaaacaac
    28441 agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc atttaaagta
    28501 aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac aggtcggcta
    28561 ccagaactcc cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct tcctgcggga
    28621 aaatggcgtt ggcgacagcc tgttccagtg cattgccttc cagattttga gtttcaacat
    28681 catgcagacg cagtgcttta ctggcgacgc catccagcgc aattcgcata ccgtcgtggt
    28741 ctgttattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg ctaattttgc
    28801 gagtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttaacagg cgatcgcatg
    28861 ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc agttcagcat
    28921 ccagtgccag caggacggga agcagcactg actcttcttg ccgggcggca atttcaccac
    28981 caatggtcga ctgattacga acatggcgag agtagacaaa accgagagct tcccgcagtg
    29041 ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg agacattgcg ccaattcgta
    29101 gtgcaccgtt atcccagtca atccagtcca gctccagatc ctgcaaggaa atggcaatct
    29161 ttttatcggt acgggttggt gtagcgttga gtttgctgcc cccggcgaac cagacggctt
    29221 catcctggta gcggcgcttc agttccagcg cctgttcgac agagtcgggt ctgaaaaatt
    29281 gttcaatcat cgtctttcct taatttcccc tcttctctga aagaagaggg gagctgttat
    29341 ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg cttgcgcata
    29401 aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacac cattgaccat
    29461 cacgctgtga acgctacctg agcccatacc gaaagcgata tgcccggcga tattgtctgc
    29521 cagcagcggc gtcggcgagt tgtaatcgca aatggttaaa tcggctttgt aaccggcttc
    29581 cagaagccca aatttcgcgc caaagttgcg gctcatcagt tcgttgccgt tagccagtgc
    29641 tttggcaaaa ctgtcaggcc acagcgggcc acccgcatcg cgatgtttaa agaaggcaaa
    29701 tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt cccaacgcca gattgcggat
    29761 gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg cgttatgcac
    29821 caggaacgca tcgcgctgat tgagtaggac gatgtcatct ttcgacaggt acagcccatg
    29881 agcgaccagc gttttgctgt cgatgagatc gaattgcgca agtcgtgcca gcaggtcttt
    29941 gccgtaccaa tggtgactgt aggaaacgtc gtaaaggtct tccgcaacgt gaatatgcaa
    30001 accacgacct gtgcttttca cggcttcacg cagcatctcc agaccggcat ccggcacggt
    30061 aaacggcgcg tgagcgccga tatgtgcttc caccagatac ggctcggttg ctgctttctt
    30121 cgcctcatca atctgacggg tgaagcggat gttttcttct acaccttcct gcaactcttt
    30181 gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc caacttttaa
    30241 aaatgcgttg cgcaatgtgg agagcgaccc gtcgatatac gccggagagg catggtgatc
    30301 gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagtc cgctgtaata
    30361 gagcgactct tcatcaagag cgcgatcgag ccgccaccag agatttttca gcgttgagat
    30421 gaaatccggg cagggggcga tgtttgccat aattccgcgg gaaagccccg agtaaaaatg
    30481 gttgtgcgag cagacaattc ccggcatcac aatccgacca tgcatctctt tgtagctggc
    30541 atcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacatcat tttcgatggc
    30601 gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc actgcggtga cattcttcag
    30661 aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg gtggacatgg
    30721 ctaatgatgc ggcacatatc gttcagctcc ggtggtacgt tgttgaactg accttcgctg
    30781 tcgatgttta acacccagct ttggttattc agacgtacgc gtacccggca atcttccaca
    30841 aggaagcctg ggttactgct gttatcaaag tcttgcgaca ggctgaagac ggtgattttg
    30901 tctttgtacg gtttaccgtt ccacgggcag aattgggcgc agttgccgca ttcgttgcag
    30961 taggcatcga gatgcagcgt ctggaaacgg ttctggaagc caggaaccgc aatggatacg
    31021 ttggcgcggt tcgggcagac atccacacac ttgctgcaaa cgtagttaca ttcaaggcag
    31081 cgtgcggctt cctgtgcgac aaacgcgtca cgatcgtcac tgttcaccag agtgacagag
    31141 atatcgcctt tacgttgata gatttccgcc ggatttacgt tgttccagta tttatcgttc
    31201 tggtgggaac ggatattttc ccgactaagg atggtatcgg tcgcccgacg cgcggttccg
    31261 acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa gacgtcagtc
    31321 agacgcgttt cgccattatg gtcgacgtct ggccagccgt ttttgtccag cggcacgccc
    31381 atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag actgtccaca
    31441 tgcagtgtta ctgtttcgtt ggtttcaacc ggacggcgac gacctttctc atcaggttcg
    31501 ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc cggattattc
    31561 agaaaacgga actccacgcc gtcgtgcaac gcttcttcat actcttcgcg ccatgcgggc
    31621 atttcttgca gtgaacgacg gtaaacgacc gttgcttttt ctacgcctgg aacgcgtaac
    31681 gccgcacgag cgcagtccat tgcggtgtta cccgccccga caacgaccac atgtttgccc
    31741 agcttgagcg cggtgccctt gttgtattca cgcaggaaag ggagtgattt ccagacattt
    31801 tggttgtcgc ccgccagttt cacaccgcta tttttatcag tgccggtggc aatcagaaca
    31861 tagtggaagc cctgattttt taactgttca acggtcaaat cgggtgagca accgtactca
    31921 aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct gaattaactc tgcaggaata
    31981 cggaactgag gaatgatatt tttcaccacg ccgcccgcat tggcttcgcg ttcaaacagc
    32041 gtgaccggat ggcccgctct ggcaaggaag taacctgcgg ccagacccgc cggacctgca
    32101 ccaatcacgg ctaccggatg gcgtgaaccg gaaccggctg gtttgtgcca gcgttgctta
    32161 tattcatccc aacctttttc cagggcgact tttttcagtt cgcggatatt cagcgcactg
    32221 tcgtaatcca ggcgggtaca gttgtattgg cactggtgat cgcaaatatg accggtaatg
    32281 gcgggcaggg cgttgcgttg atagatgagt tccagcgcgt cggcatagcg gtgttcgcca
    32341 agcagacgga tgtattccgg aatatcttgc ttgatagcgc aggcagtaac acagggggca
    32401 acgtagcaat cggtcagcgg caggtcttca gccacttcaa tacgctcttc tggcttccag
    32461 tgtttctggg tgtattccat ggttaacgcg tctgctgcca gtctgttcag tcgttcgacg
    32521 tcaacgtggt taagctccca ggcgtcggag ccttccagct cgcgcatgca ggcacttaag
    32581 cgcagatagc cgccaggttt cagcaggtcg gttgccatag taatagggcg aataccagta
    32641 tcaaaaatat cgcggatagt cagctgactg gcaccaccgg aataagaaat gggcagtttg
    32701 ccgtcaaagg cgcgagagag aactgctgca acgttgatgg agagcgggaa cagcgcacgg
    32761 cctgacatat acatctcttc accgggcagt gcgcctttat tgttgatggt gccgagagtg
    32821 ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg ccagtgccat caggcgttcc
    32881 agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa atgactcttc ttttaagccg
    32941 atgtaaccga aaccgcagac atcgaggatc tcacgaacac gcgcgtaccc cagtaaggtc
    33001 gggttgagtt tcacaaaggt gttgagtcct ttttcttcca gcatgtagcg gcaaatggct
    33061 tcgatttcat gcggaggaca gccgtgcatg gtggagaggg taacaccttg taccatgctg
    33121 gtggggatgc gagcgggtaa ggcttgcaag ctttcgcgtt tttcctgcaa tccgtggcgg
    33181 gctaaaaatg cctcatcctg gagtagctta ttaagtgcat cgcggtattg agcgaatttc
    33241 ggatggtcag atgcgtccat catattgtcg atgaactgct gcatcggcgg ttgcttaata
    33301 ccttcgaggt tgtaaccgac gctcatatta aagatgaacg atttaccgga atcagaaggc
    33361 tggaacatcg cttcgagaag gtgcagggca aaccaggctt tgaggtattc atcccaggct
    33421 ttaagcaggg taaattcggt agaccattcg gtgttaaagc actcgtcttc ggcatcgata
    33481 cagggctttt ccagctccag acggtcaaga atttggacgg tttttagttc gatgaagcgt
    33541 ccgccagtca gccaggaagt gacaatattt tgcgcgagtt gcgtgtgcgg gccagcggca
    33601 gggccgacgg gagtggcaca ggtttcaccg aagacgctaa cagttttact ttttacgggt
    33661 gagtaaaact gttgctcggg aataccaaag attgagcgtt gttgttggta ttcatcaaat
    33721 atgcgcgtca aaagttcctc aaacggaatg ggacgcataa tatcccccat aaccctctcc
    33781 ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt ttagggggat
    33841 agagcaacaa tcgcaccact cttgccaggt ttgacgggaa atataaaaaa atgtgaggcg
    33901 gctcgaaact taatggcttt ttgtgaatga actcacaggt gccaaatgag aattaaaatc
    33961 actcctgaaa attattattg ccagcccatt tatcactttt tct
//
LOCUS       DACADA010000033        33874 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.033, whole genome shotgun sequence.
ACCESSION   DACADA010000033 DACADA010000000
VERSION     DACADA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.033"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            93..1067
                     /gene="hemB"
                     /locus_tag="ISB17_001600"
     CDS             93..1067
                     /gene="hemB"
                     /locus_tag="ISB17_001600"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAP1979544.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1107..1958)
                     /gene="tauD"
                     /locus_tag="ISB17_001601"
     CDS             complement(1107..1958)
                     /gene="tauD"
                     /locus_tag="ISB17_001601"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAP1979545.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1955..2782)
                     /gene="tauC"
                     /locus_tag="ISB17_001602"
     CDS             complement(1955..2782)
                     /gene="tauC"
                     /locus_tag="ISB17_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAP1979546.1"
                     /translation="MSVLINEILHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2779..3546)
                     /gene="tauB"
                     /locus_tag="ISB17_001603"
     CDS             complement(2779..3546)
                     /gene="tauB"
                     /locus_tag="ISB17_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAP1979547.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3559..4521)
                     /gene="tauA"
                     /locus_tag="ISB17_001604"
     CDS             complement(3559..4521)
                     /gene="tauA"
                     /locus_tag="ISB17_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1979548.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4827..5102
                     /gene="frmR"
                     /locus_tag="ISB17_001605"
     CDS             4827..5102
                     /gene="frmR"
                     /locus_tag="ISB17_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HAP1979549.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            5137..6246
                     /locus_tag="ISB17_001606"
     CDS             5137..6246
                     /locus_tag="ISB17_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HAP1979550.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            6339..7172
                     /gene="fghA"
                     /locus_tag="ISB17_001607"
     CDS             6339..7172
                     /gene="fghA"
                     /locus_tag="ISB17_001607"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP1979551.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(7297..7836)
                     /locus_tag="ISB17_001608"
     CDS             complement(7297..7836)
                     /locus_tag="ISB17_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HAP1979552.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7938..9149)
                     /gene="mhpT"
                     /locus_tag="ISB17_001609"
     CDS             complement(7938..9149)
                     /gene="mhpT"
                     /locus_tag="ISB17_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HAP1979553.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(9325..10338)
                     /gene="dmpG"
                     /locus_tag="ISB17_001610"
     CDS             complement(9325..10338)
                     /gene="dmpG"
                     /locus_tag="ISB17_001610"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAP1979554.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGGDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(10335..11285)
                     /locus_tag="ISB17_001611"
     CDS             complement(10335..11285)
                     /locus_tag="ISB17_001611"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAP1979555.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(11282..12091)
                     /gene="mhpD"
                     /locus_tag="ISB17_001612"
     CDS             complement(11282..12091)
                     /gene="mhpD"
                     /locus_tag="ISB17_001612"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HAP1979556.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(12101..12967)
                     /locus_tag="ISB17_001613"
     CDS             complement(12101..12967)
                     /locus_tag="ISB17_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxononadienedioate/2-hydroxy-6-
                     oxononatrienedioate hydrolase"
                     /protein_id="HAP1979557.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12985..13929)
                     /locus_tag="ISB17_001614"
     CDS             complement(12985..13929)
                     /locus_tag="ISB17_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HAP1979558.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13931..15595)
                     /locus_tag="ISB17_001615"
     CDS             complement(13931..15595)
                     /locus_tag="ISB17_001615"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HAP1979559.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            15786..16619
                     /gene="mhpR"
                     /locus_tag="ISB17_001616"
     CDS             15786..16619
                     /gene="mhpR"
                     /locus_tag="ISB17_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HAP1979560.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLLEEGYVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            16696..17778
                     /locus_tag="ISB17_001617"
     CDS             16696..17778
                     /locus_tag="ISB17_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1979561.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            17901..20975
                     /locus_tag="ISB17_001618"
     CDS             17901..20975
                     /locus_tag="ISB17_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP1979562.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFL
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            21027..22280
                     /gene="lacY"
                     /locus_tag="ISB17_001619"
     CDS             21027..22280
                     /gene="lacY"
                     /locus_tag="ISB17_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HAP1979563.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLVAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            22346..22957
                     /gene="lacA"
                     /locus_tag="ISB17_001620"
     CDS             22346..22957
                     /gene="lacA"
                     /locus_tag="ISB17_001620"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HAP1979564.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGCHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(23078..24361)
                     /locus_tag="ISB17_001621"
     CDS             complement(23078..24361)
                     /locus_tag="ISB17_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HAP1979565.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(24351..25610)
                     /gene="codB"
                     /locus_tag="ISB17_001622"
     CDS             complement(24351..25610)
                     /gene="codB"
                     /locus_tag="ISB17_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP1979566.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(25935..27821)
                     /gene="prpE"
                     /locus_tag="ISB17_001623"
     CDS             complement(25935..27821)
                     /gene="prpE"
                     /locus_tag="ISB17_001623"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HAP1979567.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(27861..29312)
                     /gene="prpD"
                     /locus_tag="ISB17_001624"
     CDS             complement(27861..29312)
                     /gene="prpD"
                     /locus_tag="ISB17_001624"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HAP1979568.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(29346..30515)
                     /gene="prpC"
                     /locus_tag="ISB17_001625"
     CDS             complement(29346..30515)
                     /gene="prpC"
                     /locus_tag="ISB17_001625"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HAP1979569.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(30675..31565)
                     /gene="prpB"
                     /locus_tag="ISB17_001626"
     CDS             complement(30675..31565)
                     /gene="prpB"
                     /locus_tag="ISB17_001626"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HAP1979570.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            31804..33390
                     /gene="prpR"
                     /locus_tag="ISB17_001627"
     CDS             31804..33390
                     /gene="prpR"
                     /locus_tag="ISB17_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HAP1979571.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(33491..33766)
                     /locus_tag="ISB17_001628"
     CDS             complement(33491..33766)
                     /locus_tag="ISB17_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1979572.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
BASE COUNT         7844 a         9464 c         8865 g         7701 t
ORIGIN      
        1 cttcttgtca acatcgcgac aactttcgta aaacacaccc agcctgcttc aggtatacta
       61 tgcccctcga ttccacaaac atcaggcaga ccatgacaga cttaatccaa cgccctcgtc
      121 gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt agccttaacg
      181 acctggtgtt gccgatcttt gttgaagaag aaattgacga ctataaagcc gttgaagcca
      241 tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa cgcatcgcca
      301 acgccggtat tcgttccgtg atgactttcg gcatctctca ccataccgat gaaaccggca
      361 gcgatgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc aagcagaccg
      421 tgccagaaat gatcgttatg tcagacacct gcttctgtga atacacatct cacggtcact
      481 gcggtgttct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc
      541 aagccgtggt tgcagctgct gcaggcgcag acttcatcgc cccttctgcc gcgatggatg
      601 gtcaggtaca ggcgattcgc caggcgctgg acgctgcggg ctttaaagat acggcgatta
      661 tgtcgtattc gaccaagttc gcctcctcct tttatggccc gttccgtgaa gctgccggaa
      721 gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt cgtgaggcga
      781 ttcgtgagtc actgctggat gaagcccagg gcgcagactg cctgatggtt aaacctgccg
      841 gagcatacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt
      901 atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt gctatagatg
      961 aagagaaagt cgtgctcgaa agcttaggtt cgattaagcg tgcgggtgcg gatctgattt
     1021 tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaatga tcattatgcc
     1081 gggttatcca acccggcatt tcccccttac cccgcccgat aaaacggaat atccccaaga
     1141 atcgtcgccc gatgcataat ccgccgctgt ggcaggtaat ccgcattggc atagtgctga
     1201 gtcactcggt tatcccaaat cgcaatatca ttcggttgcc agcgccagcg cacctgaaac
     1261 tccggtttag tgatatgggc aaacaaaaaa cctaacaagg cttcgctctc tttctcgctg
     1321 acatcaacaa ttcgtgtggt aaaaccttcg ttcacaaaca gtgcctgttt accggtcacc
     1381 ggatgcgttc gcaccaccgg atgtagcaac ggtggatttt tcgcgaccgc ctcacgccag
     1441 cgttgatgct cagcctcggt tttgcggtac ttgtattccg ggaacgattt acggaaatca
     1501 tgctccgcac gcaacccgct caacagctgg cggaagggaa cagagagcgc ctcatatgcg
     1561 gcaataccac tggcccagag cgtatcgccg ccggtcgagg gtaactcttt cgccgccaga
     1621 atcgcccctg cgggtggcgt ttgaataaat gtcacatcgg tgtgccagtt gtcgttatcc
     1681 ggcggattat cattatgggt atccaggacg atgatctcgt caaccccttc agcatgaggg
     1741 taaacagggt gaatatgcag ctcgccaaaa cgctgggcca gcgcgcgttg ctgctgcggc
     1801 gtgatagctt gatcgcgcag aaacaccacc tgatggcgca gcaccgcatg gtaaagctgt
     1861 tcaaactgat tatcgcttaa cgggcgcgtt aagtcggccc ccgtaatttg tgcgccaata
     1921 tacggcccca gcggggtaat gctcagacgt tcactcattg tacttctcca tgccagggcg
     1981 tcaggcggcg ctgtaacgcg cgcagaccca gttctaaaag aaaggcgata atcgcaatca
     2041 ccgcgatccc cgccagcacc acgtcagtcg cgagaaattc acccgctgac tgaaccataa
     2101 atcctaaacc gcgcgttgcg gcaatcagct ccgccgccac cagcgtagac cagcccacac
     2161 ccagaccaat gcgtaatccg gtgaggattt ccggcagcgc gccgggcaaa atgacaaacc
     2221 acagcacctg cgcacggctg gcacccagcg actgggcggc acgaatgcga acctgctgcg
     2281 cgcttttcac ccccgccagc gccgacatcg ccaccggcgc aaaaatcgct aaatagatca
     2341 gcaagatctt cgaggtttcg ccaataccaa accagatcac catcagcggc aaataagcca
     2401 gcggcggcac cggacgataa agctcgatta tcggatccag aatgccgcgc accgtcgggc
     2461 ttagccccat ggcgatcccg accggaatac cgatcaacac cgccgcgagt agcgccagca
     2521 aaatgcgcgt caggctggct gccagatgct gccacagcgt ggcgtccata aatccctgcg
     2581 gcccggcaat ggtgagcagt tttgccagta cctgttgcgg tggcggcaaa aacagcgggc
     2641 taatcagttg cagcgccgcg actgcccacc atacagtgag caaaaccgct aacgtaccaa
     2701 tgcttaaggt cacctgacgc gagagcggcc agcgccattt cagccgatgc gaatgcagta
     2761 tttcattaat gagcacactc atgagaacgc ctcccgttgc tcaaatacgc ggcttaaaac
     2821 atattcgcgc atggcgatga attgtggatc ggacttgatg ctgcggctcg actctcccgc
     2881 aacaaagcgg cgagcaaagt tgagcggcag ccgctccagc acacggcctg ggccgggtga
     2941 aagcaaaacc agttcagtcg ccataaacac cgcttcttct atatcgtggg taatcaacag
     3001 cacctgcttt cccgtctcct gccaaagttt cagcagcagg gtttgcatct ggtcgcgggt
     3061 gaaggcgtcc agcgcaccaa acggttcgtc gagtaataac agctggggat ttgccgccaa
     3121 cgcgcgggca atccccaccc gctgacgttg accaccggaa agctgccaga tgtagcgttt
     3181 ttctgcgcct tccagcccga cttttttcac catctggtgt gcgatttcca gtcgctgcat
     3241 tttctctaca cctgccagtt gcaggccgaa cgccacgttg tcctgtacat tgcgccacgg
     3301 taacaaccct tcattctgaa aaactacgcc acgctctgct cccggtccct gaataccctt
     3361 acccgccagt tgaatgctgc catgctgata aggcacaaaa ccagcaatca aattcagcaa
     3421 cgtagttttg ccgcagccag acggccccag aactaccaga agctcgccgc tttccagcgt
     3481 caggttgata tcttccagtg ccggtttgcc gccataatcg gcgtaaagat gagaaatttg
     3541 cagcatctgc gcctcctttt attgcacgaa gcgcgaggta acgtactggc tgtaatcatt
     3601 cgctacagcc gggactttgc cctgctcttt caaaaactgc gcggtgtcga tgatcgcttt
     3661 gttcaccggt ccggtcagtt ctgccgtttg ttgctgcggc gtcagatagg tattcccctt
     3721 caccagcccc ggaacgtcac cttccggcac gccgcttaac cgcgccagtt tgctgatgtt
     3781 ttccggctgt ttcagccacg cgtctgggtt agcaatgtac ggttgctgag catcgatggc
     3841 gcttttagcg aacgctttca cgacctcagg atgtttctcg gcaaaatctt tccgcacgac
     3901 ccagacgtcc agtgtcggcg cgccccactg cccgacctgt tcagaatcgg tcagcacctt
     3961 gccatctttt tccagggcgt taaccgccgg tgcccataca taagcaccat caatatctcc
     4021 ccgctgccat gcggcgataa ttgcgggcgg ctgcaggttc acaatctcaa cttgcccagg
     4081 tttaataccc cagtgtttca gcgccgccag caggctgtag tgggtggtgg agataaacgg
     4141 tacggcgatg cgtttaccaa tcaagtcttc cggtttactg atagttttct ttaccaccag
     4201 cgcttcggag ttacccagtt ttgacgccag caagaagact tcaatcggca cctgttggct
     4261 ggttgcaacc gctaacgggc tggaaccgag gttgccgatt tgcacatcgc ccgaagccag
     4321 cgcccgcacg atgctggctc cactgtcaaa cttacgccag tccacggttg ctccgctttc
     4381 tttagcaaag gtgttgtcgg cctgagccac tttcgccggt tcggcggagg tttgatacgc
     4441 cacggtgacg ttcaccgctt gcgcctgaaa agcgatgaat gccagtgcgg caagaaatgt
     4501 gttacgcgat gaaattgcca tgattgtctg ctcccccgtc ttgttatggg agcagtattt
     4561 cgttgcgggt aagtttgata aaggaattaa aacgtcttgt taataacaaa gcgtttttag
     4621 aaaaaaatcg gcaatggtta gcgagtttgc gcattttgct acttttaaca aacatcgcct
     4681 gatgtgacgc tcacgcctct tatcatgcct acaaatactg gggccggata aattttattc
     4741 tccagtgtta tatactatag gggggtatgc attgacatat agcatacccc cctatagtat
     4801 attgcgtgca gatgatgagg tgcgaaatgc ccagtactcc ggaagagaag aaaaaggtcc
     4861 ttactcgagt tcgtcgtatt cgggggcaga ttgatgctct ggaacggtcg ctggagggtg
     4921 atgccgaatg ccgtgccata ctccaacaga tcgctgccgt tcggggcgcg gctaacggac
     4981 tgatggcaga agtgcttgaa agccatatcc gggaaacgtt tgaccgaaat gactgctaca
     5041 gccgtgaagt cagccaatcc gttgacgaca ctattgaact ggttcgtgcc tatcttaaat
     5101 agctgaatct attaccatat tgaggaagag cgagagatga aatcacgtgc tgccgttgca
     5161 tttgctcccg gtaaaccgct ggaaatcgtt gaaattgacg ttgcaccacc gaaaaaaggt
     5221 gaagtgctga ttaaagtcac ccataccggt gtttgccata ccgacgcatt taccctctcc
     5281 ggcgatgacc cggaaggtgt cttcccagtg gttctcggtc acgaaggggc cggggttgta
     5341 gttgaagtcg gtgaaggcgt aaccagcgtc aaacctggcg accatgtgat cccactttac
     5401 accgcagagt gcggcgagtg tgagttttgt cgttctggca aaaccaacct gtgtgtcgcg
     5461 gttcgcgaaa cgcagggcaa aggcttgatg ccagacggca ccacccgttt ttcttacaac
     5521 gggcagccgc tttatcacta catgggatgc tcaacattca gtgaatacac cgtggtagcg
     5581 gaagtgtctc tggccaaaat taatccagaa gcaaaccatg aacacgtctg cctgctgggc
     5641 tgtggcgtga ccaccggtat tggcgcggta cacaacacag ctaaagtcca gccaggtgat
     5701 tctgttgccg tgtttggtct tggcgcgatt ggtctggcag tggttcaggg cgcgcgtcag
     5761 gcaaaagcgg gacggattat cgctatcgat accaacccga agaaattcga actggcgcgt
     5821 cgcttcggtg ccaccgactg cattaacccg aatgactacg acaaaccgat taaagatgtc
     5881 ctgctggata tcaacaaatg gggtatcgac catacctttg aatgcatcgg taacgtcaac
     5941 gtgatgcgtg cagcgctgga aagcgcgcac cgcggctggg gtcagtcggt gatcatcggg
     6001 gtagcaggct ccggtcagga aatctccacc cgtccattcc agttggtcac cggtcgcgta
     6061 tggaaaggtt ccgcgtttgg cggcgtgaaa ggtcgttccc agttaccggg tatggttgaa
     6121 gatgcgatga aaggtgatat cgatctggaa ccgtttgtca cgcataccat gagcctggat
     6181 gaaattaatg acgccttcga cctgatgcat gaaggtaaat ccattcgaac cgtaattcgt
     6241 tactgatttc ccgctggttt accccgtcca gttcagatgg ggttcttaat actctccccg
     6301 ggcagccgtc cgggggatta accgtgagat aatgactgat ggaactcatt gaaaaacatg
     6361 ccagctttgg cggctggcaa aatgtgtatc ggcattattc ccaatcactg aaatgtgaaa
     6421 tgaatgtcgg cgtctatctc cccccaaaag ccgagaatga aaaattgccg gtgttgtact
     6481 ggctttcagg cctgacctgc aacgagcaaa atttcattac taaatcgggg atgcagcgtt
     6541 acgcggctga gcacaacatt attgttgttg cgccggacac cagtccgcga ggcagtcatg
     6601 tcgcagatgc tgaccgttac gatctcgggc aaggtgccgg gttttacctg aacgcgacgc
     6661 aagcgccgtg gaatgaacat tacaaaatgt atgactatat ccgcaacgag ctgccgaatt
     6721 tagtgatgca tcattttccg gcaacggcca gaaagtcaat cgcaggtcat tctatgggcg
     6781 ggctgggcgc gctggtgctg gcgttacgta acccagatga atatgtcagc gtctcggcgt
     6841 tttcgcccat tgtctcccca tcgcaagtac cgtggggaca gcaagccttt gccgcatatc
     6901 ttggtgaaaa taaagatgcc tggttggatt acgatccggt gagtcttatt tcacaaggtc
     6961 aacgcgttgc ggaaatcatg gttgatcagg ggttgagtga tgatttttac gcggaacaac
     7021 tgcggactcc aaatcttgaa aagatctgcc aggagatgaa tatcaagacg ttaatccgtt
     7081 atcacgaggg ttatgatcac agctattatt ttgtctccag ttttattggc gagcatattg
     7141 cctaccacgc caataaactg aatatgcgtt gagcatccgc gcacgcatgc cggatgcggc
     7201 gtaaacctta tccggcccac atttcgctcg taaaccgtag gcctgataag acgcgaaaag
     7261 cgtcgcatca ggcatctgca cacgatttat caatgtttac cacatcaaat catcaggcac
     7321 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcgctgagcg cgctgtgcaa
     7381 cacaatactg ctggcatcac gctgagcaat tttatcggca acgctggcgg gaataatggc
     7441 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     7501 caactgcgtt tgcgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     7561 gttaaaacca atatcgccat tggcaatggt aattcggttc atttcaatga gctgcttcac
     7621 ctgggcttta tattctttcg ccaacgccgc ttgtttttgt tgctcgctaa gctgtttatc
     7681 acgctcaagc tgtgcctttt tattttcttc taccgccgcc cgtgcttcac gcgcctgaac
     7741 acgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctagtcccgc
     7801 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7861 atttttagcg agtatacctg taaacgccgt aggtgtattg ggggcaagtc cctttcgccc
     7921 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7981 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     8041 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     8101 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     8161 aacggtgcca gagcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     8221 cccgccagca acatactgtt aaatgacgac accgttccca gcgccagcag cgaagctaac
     8281 atgccgctat aaatcagtag cgacatggtt actggacgca gtttatccat caatgcgccc
     8341 aacattaacg tcccgcttgc cgcccccatt tgcagtgcaa acatcacccc tgccgcctgc
     8401 gatggctgga atccttgctc caccaaaagc agcggcagcc agttgatcaa catgtagacc
     8461 accagcagag tgaaaaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     8521 gcaaataacg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgtcgactcc
     8581 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     8641 accgtttgcc atgctaagtt tgcccccgcg aaacccagcg tcgccgccag cgccgcgcca
     8701 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg tcccgcggct
     8761 tcagacgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     8821 cgcgcaaaga ccagtgaggg gaaatcccat gcaatcgccg ttgccagcga gaacaaacca
     8881 aacagcgcaa ctgagccaat caaaatacgc ttacgcccat aacggtctgc cagcatcccg
     8941 ccaaccagcg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     9001 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     9061 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     9121 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     9181 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     9241 atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgctgggat gggcatacaa
     9301 aatgcacgtt ctcggcagtc aatattattt gttgttgcgc agatccagcg ccacgtcgac
     9361 gatcatatcc tcctggccgc caaccatccg gcgtttgccc agctcaacga gaatatccac
     9421 cgcgcttaag ccataacgcg ccgccgccgt ttcacagtga cgcaggaagc tggagtaaac
     9481 accagcgtat cccagcgcca gcgtttcgcg atcgactcgt accggtcgat cctgcaacgg
     9541 acgcaccagg tcgtcagcgg catccattaa cgcatagaga tcgcccccat gctgccaacc
     9601 cagtttatcc gcggcggcaa taaatacttc cagcggcgcg ttacctgcgc ccgcgcccat
     9661 tcccgcgagg ctggcgtcga ttcggtcgca gccctcttcc accgccgcga tagaattcgc
     9721 cacgccaaga ctcaggttat ggtgagcgtg catgccggtt tgcgtttctg gtttcagcac
     9781 tgctttcagg gcgcggaaac ggtcacggat atcgctcatg ttcatcgcac cgccagaatc
     9841 caccacataa atacaggtcg caccgtagcc ttccatcagc tttgcctgct ttgcgagatt
     9901 ctccggcgtg gtcatatggc tcatcatcag aaaaccaacg gtgtccattc cgagctcgcg
     9961 ggcatactga atatgctggg cggaaacatc agcctcggta cagtgcgttg ccacacgaac
    10021 cacccgcgcg ccagcctgcc aggcattttt cagatcgtga atagtgccga ttcctggcag
    10081 caacaacgtc gcgattttgg cgtgcttcac cacatccgcc gccgcttcaa tccattcaag
    10141 gtcgctatgt gcgccgaaac catagttaaa gctggaaccc tgcaaaccgt cgccgtgggc
    10201 cacttcaatc gaatccacgc gggcatcatc cagtgctttg gcaatctggc ggacattttc
    10261 cagcgaatac tgatgacgaa tggcgtgcat accgtcacgc aacgtgacgt ccgaaatata
    10321 aagtttttta ccgttcatgc cgcttctcct gccttgcgcg ccagtgactg ggccattttt
    10381 tccgctgtcg ccagcgcact ggaagtcata atgtcgaggt tgcccgcata ggcaggcaga
    10441 taatgcgctg cgccttcgac ttccagccag accgccgttt tcagcccgga gaactgcccc
    10501 acgcccggta aattgaccgg tttatcctgc gggatgactt caaactgcac gcgctgtttc
    10561 aggcgataac ccggtacgta agcctgcacc gcctcagcca tttcattgat tgaggcttcg
    10621 atatcatctt gtgaagcttc gtcgctcaat acatacaccg tgtcacgcat catcaacggt
    10681 ggctctgctg ggttaagcac aataatcgcc ttcccttttg ccgcgccacc caccacttca
    10741 atggcgcgcg aagtggtttc cgtaaattca tcgatgttgg cacgcgtgcc gggtccggca
    10801 gatttactgg caatagaagc gataatttcg gcgtaatgaa cacgcgccac gcgtgaaact
    10861 gccgctacca ttggaatggt ggcctggccg ccgcaggtga ccatgttgac gttcagttga
    10921 gcgacgttct cctcgaggtt aaccaccggc acgcagtaag ggccgatggc agcaggcgtc
    10981 aggtcaatta agcgaatatc cggtttcgct tcgcgcaaag cggcatcgtt tttcacatgc
    11041 gcgcccgcgc tggtcgcatc aaatacaatg tcgatatcag caaattcagg catgttcatc
    11101 agcccgatca ccccttcatg ggtggtggcg acgcccatac gtctggcacg cgccagaccg
    11161 tcggactgag gatcaatgcc gaccatcacc gccatctcca gatgctgacc gtgacgcaaa
    11221 attttaatca tcagatcggt gccaatgttg cctgaaccga taatggcgac tttacgctta
    11281 ctcatgacag acttcctttt ggggctgcgc ttgaaaatgt cgcagcaact gaacctatgc
    11341 cttcgatatg ggcttcaaaa cgatcgcccg cattcaccgc caccatcgga cctaatgccc
    11401 cggtaagaat gatatctccg gcgcgcagcg gttcacccag actggccatt ttgcgtgcca
    11461 gccagacggc ggcattaagc ggatgtccca ggcattcgct gccgcgcccg ctggaaacct
    11521 cttcgttatt acgcgtcatc ttcatggcgc agtttttcag gtctaacccc gccgggcgtt
    11581 gcgccggacc gccgatgaca tacaccccgc aggaagcgtt atctgccacg gtatcgacaa
    11641 actgaatcga ccagtcgcga atgcggctcc ccaccacctc cagcgccgga agtacccatt
    11701 caatggcgtt atacaattcg tcgaaggtga tatcggttgc gggtagatcg cggttcaaca
    11761 ccagtgcaat ctccgcttca atgcggggtt gcagaacacg ggaaaaagga atggtttcat
    11821 tatcgccata acacatgtcg gcaaataatg tcccgaaatc cggttgatca acgcccagtt
    11881 gttgctgcac tttcggatgc gtcagcccca ctttgcgccc caccacgcga cgcccctgcg
    11941 caacgtcatg ttgcacattg atgtgctgaa tggcgtaggc cgcttcagcg ttatcgatac
    12001 caatcagatc gcgcagcggc gcaatcgctt cgccctgctc tgcggcgcgg cgtaaatccg
    12061 ccgccagttg ctcaagagta tgcttcgtca tgaccgttcc ttaagggcgt gcgaggaaat
    12121 tcagcaccag ttgattgaaa gcgtcggcat gttcccactg cgcccagtga ccacagtcgc
    12181 ggaagatatg cagttcagaa ccggcaatgc cggacagcag acgcagaccc gcatccatcg
    12241 gcacaaagcg gtcgttgcgc ccccagacaa tcagggtttg cgctttgatt tccgccagac
    12301 gtgggccaaa atccgggaac tgtttcggat tggcttccag gctcttaacg aagttttcca
    12361 ggtgatcgcg gcgcgacagc atattattca ggcgcgcttc aaacagggcg tcggtcaaat
    12421 cgctggtatc aaaaacgaag atatccatca tcagcttcag gttttcgata gtcggctgac
    12481 gataaagctg attcagtcgc ttaatacctt cggttggcat cggcgtaaac aaactcatgc
    12541 cgcccgtacc gccgcccatc agcaccagtt tgccgacgcg ctccggccag ttaagagtga
    12601 acgccacaga actgtggcca cccatcgagt tgcccagcag gtggattttg gcgatatcca
    12661 gttgatccac cacgcttttc aggattcgcg cattaagatc cgatcgcgaa ccactattaa
    12721 cgatcgaatc gctcttaccc caacccggac aatccagcag gatcacccga tagcccgcct
    12781 ctaccagcgg atcgatattg cggctgaagt tcgcccagcc agtagcaccc gggccggaac
    12841 catgcagcag gacaacggtt tcgtcgcctt gtccgcagtc attaaaatgg atgcgcagcg
    12901 ttttacccgc ttcttctaca ttcagaaaac ggctggtggc ggcttcggtt tgtggctgat
    12961 aactcatcat cttctcctgc atattcagtt ctctgttctg gcgcttaacg agccaaatcc
    13021 ggcaatccac tccgggattg ggcggtaata acgcccttcg ctacgccagt tgccaaacgc
    13081 agaaatagcg gcaaaagcgg cgacccaggt tttgatttca tgtgtcgact ttccggcaat
    13141 ggcggaaagc tcttcgttac tgacggcatc cagttcctgt atgcgtccct gctccagcaa
    13201 agtcatgaac tggttatccc aaatcgggtt gagcggatgc agcgttctct gatcctcaac
    13261 aaacttctca gcggcgctaa tcacccgttg ctgacgcaat tcgcgctcac tggcgggtaa
    13321 atctttcccg ctccccaaca gacggtcgcg catatgggca tcggctttcg ccagttcggg
    13381 caccggcggc tgatgggaaa gcccaccgga acccaggaac agcacgcgtt tattgagagt
    13441 actggtgaaa cgtccaatgg cttcccccaa catgcgggta cgctggaaac cgggcagcgg
    13501 cgtggcgaca ccgttgatga acaccggcag tactggcacc ttatccagcc caccgagcag
    13561 gaactccagc ggctgggcga acccgtggtc cacctgcata cagtaagaaa cggcaagatc
    13621 gatcccgctc ttcatgacgg catgcgcaca ggcctccgcc agctccacag gcacgggcag
    13681 ctctcctgcc gcactgccga aatcaccaat tgccgtcgct ccaacgccta aacagaacgg
    13741 tggcatcacg tcatagaaaa agccgttgta gtgatctggc gcaaacagca ccaccagttc
    13801 aggggagaaa gccgcaatac gctcgcgggc gctggcaatc acgccattga cctcatcgag
    13861 tacctcttgt gccgggtcga cataccccac cagcggcgag tgggaaagac agtgaagata
    13921 agcgtgcata tcaggctacc ttttcgacag aaacgtcggc atcagggcgg gtcagcgtca
    13981 tcaccgacgc cagtttattc agggtcttgc ccagggtttg cggaatggcg gtggcggcaa
    14041 caaagcgatc cgggcgcatc accaccagcg aagcattgtg ttgcgcgaac cagctacgca
    14101 ggcgaccttg cgtatccccc acgcgtagta cgccgtcgtg gttatcctgt gcggtatgaa
    14161 tttgcacttc cggcaccacc tgaatgaagc gtgtgcccaa cgcgcgccac tgctggattt
    14221 gctcgtcgct catcccccac agtggattgc atccccagcc aattaccgcg aagttcgcgc
    14281 cgatcgcgtt atcgagtagc gtcacgtcgc cgttttccag cgtgactttc ggctgaataa
    14341 acatcttgcc gaccggagag tgcttcgctt cgccctcacg caccagcgca ccgccgtaat
    14401 attgcggcat cggcttgaag cgcatttcga ggaagtagcg ttttactggc ggcagataat
    14461 tcagcagcca ggaaacgccg tcacgtaacg taccctgcca gcgtttcggc ggagccagca
    14521 cgttgcccgc cgtcacggac aggtcaatca tcgctttggc gtgatcgcga cgttcttgtt
    14581 gataggtatc gagcagcgca tcgcgggctt tcccctggat aaccaacgcc agtttccatg
    14641 cgaggttaaa ggcgtcgcgc ataccactgt tatagccctg cccctgccac accggcatga
    14701 tgtgcgcggc atcgcccgcc agcagtacgc gatcaatacg aaaacgttgc gccagtcgcg
    14761 cgttgtgggt gtagacacgc tggcgaatca attcaacatt gtccggatta ggcagcactt
    14821 tgcttaacag cttgcgcata ttttgcggct cacgcagctg ttcttcggtt tctcccggca
    14881 tcaccataaa ttcaaagcga cgtaccgcat gaggcagcgc ggcagaaaca tacgggcgca
    14941 ccggatcaca acacaaatag atatgcggcg tacttaacgg atcgttggcg atatctacca
    15001 caatccactg atttggcgca gttttacctt caaacggcac attcagggtg cgacggacaa
    15061 aacttgcccc accatcacag gccaccagcc actgggcttt gaccgtttcc cgctgccctt
    15121 cttccgtttt caggtgcaag gtcacttcgt catcttgctg gctgaaggcc tccagctcgc
    15181 gggaaaacag gcagcgcaca ttcggaaaac gcgacaatcc ttccagcatc accgcatcga
    15241 cctgaggctg aataaaggcg ttacggcgcg gccagccaaa ttcatcggtc attggctgaa
    15301 tatcagcaaa acagcgacct tttggggtga gaaaacgcat cgcgtgccac ggcgtagtgt
    15361 gcggcagaac attatcgacc aggccaaccg actgcatggt gcgcagcgcc tcgtcatcaa
    15421 taccaatcgc acgcgggtag tcgatcaact tatcgagttt ctccaccacc agcacgtcaa
    15481 tgcccatctg gccgagatag ttcgccatca tcagcccgac cggaccggca ccagcgatcg
    15541 ccacctgaac gctatggtta acagcaggct ggatgtcagg gtgttgtatt gccatttcag
    15601 tacctcacga ctcggacaaa atgccgttgc gcgcacagta cagcgcaact tattttgtta
    15661 aaaacatgta aatgattttt tattgtgcgc tcagtatagg aagggtgttt tcggctacaa
    15721 tcaaaacatg cccgaatgtg caccaggtgc acaacgttgt tttaactata taaatgtcaa
    15781 ttaatatgca gaacaatgag cagacggaat acaaaaccgt gcgcggctta acccgcggtc
    15841 taatgttatt aaatatgtta aataaacttg atggcggtgc cagcgtcggg ctgctggcgg
    15901 aactcagcgg cctgcatcgc accactgtgc ggcgactgct ggagacgtta ctggaagagg
    15961 gatatgtccg gcgcagtacc tccgatgaca gttttcgtct gaccatcaaa gtgcggcaat
    16021 taagcgaagg atttcgtgac gaacagtgga tttctgcact ggcggccccg ctgctgggcg
    16081 atctgttgcg cgaagtggta tggccgacag atgtgtccac gctggatgtt gatgcaatgg
    16141 tggtacgcga aaccacccat cgtttcagcc gcttatcttt tcaccgggca atggtcgggc
    16201 gacggttacc gcttctgaaa accgcctcgg gcctgacctg gctggccttt tgcccggaac
    16261 aagaacgcaa ggaattaatc gagatgttag cctcccgccc cggtgatgac tatcaactgg
    16321 cacgagaacc gttaaagctg gaagccattc tggcgcgcgc gcgcaaagag gggtacggac
    16381 agaactaccg cggctgggat caggaggaga agatcgcctc tatcgccgta ccgctgcgca
    16441 gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat ggcgagcgca atgaccatcg
    16501 aacaggcagc ggaaaagcat cttccggcgc tgcaacgggt ggcaaaacag atcgaagaag
    16561 gggttgaatc gcaggctatt ctggtagcgg gaaggcgaag cggcgtgcat ttacgctgac
    16621 accatcgaat ggcgcaaaac ctttcgtggt atggcatgat agcgcccgga agagagtcaa
    16681 ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg tcgcagagta tgccggtgtc
    16741 tcttatcaga ccgtttcccg cgtggtgaac caggcctgcc acgtttcagc gaaaacgcgg
    16801 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
    16861 ctggcgggca aacagtcgtt gctgattggc gttgccactt ccagtctggc cctgcacgcg
    16921 ccgtcgcaaa ttgttgcggc gataaaatct cgcgccgatc aactgggtgc cagcgtggtg
    16981 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
    17041 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
    17101 gtggaagctg cctgcgctaa tgttccggcg ttatttcttg atgtctctga ccagactccc
    17161 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcacctggtc
    17221 gcattgggtc accagcaaat cgcgctgtta gcaggcccat taagttctgt ctcggcgcgt
    17281 ctgcgtctgg cgggctggca taaatatctc acacgcaatc aaattcagcc gataacagaa
    17341 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctaaatgag
    17401 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
    17461 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
    17521 accgaagaca gctcgtgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
    17581 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
    17641 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca cccttccgcc caatacgcaa
    17701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca agtttcccga
    17761 ctggaaagcg ggcagtgagc gcaacacaat taatgtgagt cagctcactc attaggcacc
    17821 ccagtcttta cactctatgt gtccggctcg tatgttgtgt gaaattgtga gcggataaca
    17881 atttcacaca ggatacagct atgactatga ttacggattc tctggccgtc gtattacaac
    17941 gtcgtgactg ggaaaaccct ggcgttacac aacttaatcg ccttgcggca catccccctt
    18001 tcgccagctg gcgaaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    18061 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
    18121 ggctggagtg cgatcttcct gacgccgata ctgtcgtcgt cccctcaaac tggcagatgc
    18181 acggttacga cgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt
    18241 ttgttcccgc ggagaatccg acaggttgtt actcgctcac atttaatgtt gatgaaagct
    18301 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
    18361 ggtgcaacgg gcgctgggtc ggttacggcc aggacagccg tttgccgtct gaatttgacc
    18421 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg
    18481 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
    18541 cgttgctgca taaaccgacc acgcaaatca gcgatttcca agttaccact cgctttaatg
    18601 atgatttcag ccgcgcggta ctggaggcag aagttcagat gtacggcgag ctgcgcgatg
    18661 aactgcgggt gacggtttct ttgtggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
    18721 ctttcggcgg tgaaattatc gatgagcgtg gcggttatgc cgatcgcgtc acgctacgcc
    18781 tgaacgttga aaatccggaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
    18841 ttaaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
    18901 tcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgcg
    18961 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
    19021 tgcaggatat cctgctgatg aagcagaaca actttaatgc cgtgcgctgt tcgcattatc
    19081 cgaaccatcc gctgtggtac acgctgtgcg accgttacgg cctgtatgtg gtggatgaag
    19141 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
    19201 tacccgcgat gagcgaacgc gtaacgcgga tggtgcagcg cgatcgtaat cacccgagtg
    19261 tgatcatctg gtcgctgggg aatgagtcag gccacggcgc taatcacgac gcactctatc
    19321 gctggatcaa atctgtcgat ccttcccgcc cggtacagta tgaaggcggc ggagccgaca
    19381 cctccgcaac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
    19441 cggcggtgcc gaaatggtcc atcaaaaaat ggctttcgct gcctggagaa atgcgcccgc
    19501 tgatcctttg cgaatatgcc cacgcgatgg gtaacagtct tggcggcttc gctaaatact
    19561 ggcaggcgtt tcgtcagtac ccccgtttac agggcggctt cgtctgggac tgggtggatc
    19621 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
    19681 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
    19741 cgcatccggc gctgacggaa gcaaaacacc aacagcagta tttccagttc cgtttatccg
    19801 ggcgaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagttcctgc
    19861 actggatggt ggcgctggat ggcaagccgc tggcaagcgg tgaagtgcct ctggatgttg
    19921 gcccgcaagg taagcagttg attgaactgc ctgaactgcc gcagccggag agcgccggac
    19981 aactctggct aacggtacgc gtagtgcaac caaacgcgac cgcatggtca gaagccggac
    20041 acatcagcgc ctggcagcaa tggcgtctgg cggaaaacct cagcgtgaca ctcccctccg
    20101 cgtcccacgc catccctcaa ctgaccacca gcgaaacgga tttttgcatc gagctgggta
    20161 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgatg
    20221 aaaaacaact gctgaccccg ctgcgcgatc agttcacccg tgcgccgctg gataacgaca
    20281 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
    20341 ctggccatta ccaggccgaa gcggcgttgt tgcagtgcac ggcagataca cttgccgacg
    20401 cggtgctgat tacgaccgcc cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
    20461 ggaaaaccta ccggattgat ggacacggcg agatggtcgt cagtgtaggt gttgaagtag
    20521 cgagcgatac accgcatccg gcgcggattg gcctgacctg ccagctggcg caggtcgcag
    20581 agcgggtaaa ctggctcggc ctggggccgc aagaaaacta tcccgaccgc cttactgcag
    20641 cctgttttaa ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
    20701 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
    20761 gcgacttcca gttcaacatc agccgctaca gccaacaaca actgatggaa accagccatc
    20821 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
    20881 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
    20941 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
    21001 ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt
    21061 tcggtttatt ctttttcttt tactttttta tcatgggagc ctacttcccg tttttcccga
    21121 tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta
    21181 tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcgggc
    21241 tgcgcaaata cctgctgtgg attattaccg gcatgttagt gatgtttgcg ccgttcttta
    21301 tttttatctt cggaccactg ttacaataca acattttagt aggatcgatt gttggtggta
    21361 tttatcttgg cttttgtttt aacgccggtg cgccagcagt agaggcattt atcgagaaag
    21421 tcagccgtcg cagtcatttc gaatttggtc gcgcgcggat gtttggctgt gttggctggg
    21481 cgctgtgtgc ctcgattgtc ggcatcatgt tcaccatcaa taatcagttt gttttctggc
    21541 tgggttctgg ctgtgcactc atcctcgccg ttttactctt tttcgccaaa acggatgcgc
    21601 cctcttcagc cacggttgcc aatgcggtag gtgccaacca ttcggcattt agccttaagc
    21661 tggcgctgga actgttcaga cagccaaaac tgtggttttt gtcactgtat gttattggcg
    21721 tttcctgcac ctacgatgtt tttgaccaac agtttgctaa tttctttact tcgttttttg
    21781 ctaccggtga acagggtacg cgggtatttg gctacgtaac gacaatgggc gaattactta
    21841 atgcctcgat tatgttcttt gcgccactga tcattaatcg catcggtggg aaaaatgccc
    21901 tgctggtggc tggcactatt atgtctgtac gtattattgg ctcatcgttc gccacctcag
    21961 cgctggaagt ggttattctg aaaacgctgc atatgtttga agtaccgttc ctgctggtgg
    22021 gctgctttaa atatattacc agccagtttg aagtgcgttt ttcagcgacg atttatctgg
    22081 tctgtttctg cttctttaag caactggcga tgatttttat gtctgtactg gcgggcaata
    22141 tgtatgaaag catcggtttc cagggcgctt atctggtgct gggtctggtg gcgctgggct
    22201 tcaccttaat ttccgtgttc acgcttagcg gccccggccc gctttctcta ctgcgtcgtc
    22261 aggtgaatga agtcgcttaa gcaatcaatg tcggatgcgg cgcgagcgcc ttatccgacc
    22321 aacatatcag ggcggaatga tcgcaatgaa catgtcgatg acagaaagaa taaaagcagg
    22381 caagctattt accgatatgt gcgaaggctt acctgaaaaa agacttcgtg gaaaaacgtt
    22441 aatgtatgag tttaatcact cgcatccatc agaatttgaa aaaagggaaa gtctgattaa
    22501 agaaatgttt gccacggtag gggaaaacgc ctgggtagac ccccccgtct atttctctta
    22561 cggttccaac atccatatag gccgcaattt ttatgcaaat ttcaatttaa ccattgtcga
    22621 cgactacacg gtaacgatcg gtgataacgt actgattgca cccaacgtta ctctttccgt
    22681 tacgggacac ccggtacacc atgaattgag aaaaaacgga gagatgtact cttttccgat
    22741 aacgattggc aataacgtct ggatcggatg tcatgtggtt attaatccag gcatcaccat
    22801 cggggataat tctgttattg gcgcgggtag tgtcgtcaca aaagacattc caccaaacgt
    22861 cgtggctgca ggcgttcctt gtcgggttat tcgcgaaata aacgaccggg ataagcaata
    22921 ttatttcaaa gattttaaag ttgagtcttc agtttaaata agcaaaattg cctgatgcgc
    22981 tccgcttatc aggcctacaa tttcagtgat ctacgtaggc cggataaggt gttcacgccg
    23041 catccggcat aaactacgct cgcggtaacc cagccgatta ccgtttgtaa tcgatggctt
    23101 ctggctgctc cagatatacg gtggtttgtg ccggttttgt gctggcaatc accttgccgc
    23161 cacgtaccga atagcgcacc ggaacctgac ggcgcagcgc gtcaaaccca ttttcagccg
    23221 gcaggataat caggttggcg ctgtttccgg cggcaatgcc gtaatcctgc aaattcaacg
    23281 ttctggcact gtggtgggtg attaaattca ggccgtcgtt aatctgccca tagcccatca
    23341 gctggcaaac atgcagcccc atatgcagca cttgcagcat attcgccgtt cccagcggat
    23401 accacggatc gaagacatca tcgtgaccaa agcagacgtt aatgccggac tccagcatct
    23461 ctttaacgcg cgtgatgccg cgacgttttg gatacgtatc gaaacgtcct tgcagatgaa
    23521 tattgaccaa cgggttggcg acaaagttaa taccggacat tttcagcaaa cgaaacaggc
    23581 gcgaagtata cgccccgtta taggagtgca ttgccgtagt atggctggcg gtgactcgcg
    23641 cgcccatgcc ttcacggtgc gccagggcag caacggtttc gacaaagcgt gattgctcat
    23701 catcaatctc gtcacagtga acatcgatga gacggtcgta tttttgcgcc agggcgaagg
    23761 ttttatgcag cgactccacg ccatattcac gggtaaattc aaaatgcgga atcgccccaa
    23821 caacgtctgc ccctaagcgt aacgcctctt ccagcaacgc ttcaccattg ggatacgaca
    23881 aaatcccttc ctgcgggaag gcgacgattt gtagatcaat ccacggcgca acttcctgtt
    23941 tcacttccag cattgctttc agcgcagtca gcgttgcgtc cgaaacatcg acatgggtac
    24001 gcacatgctg aatgccgttg gcaatctgcc atttcagcgt ttgccatgcg cgttgtttca
    24061 catcgtcatg agttaataac gctttgcgct cggcccagcg ttcaatgcct tcaaacagcg
    24121 tgccggactg attccagttc ggttgtccgg cggtttgcgt ggtgtccagg tgaatatgcg
    24181 gctccacaaa cggcggtaaa actaaacctt gttcggcatc caggctgttt tcagttacgg
    24241 gcatcacgcc ggattgcgca tcaatggcgc tgatttttcc gtcgtgaaaa tgaatctgcc
    24301 acagcccctc tttgccgggt aaccgggcgt taataattgt ttgtaaagca ttattcgaca
    24361 ctgttagcct ccacatgcgt cattgctgct gtcgttttac gattcaaaat cgggttaagg
    24421 atcagatagc tcagcgcgcc acctaatacc gcgttgaccg gaacaattcc cggtaaccag
    24481 tggcctgcgg caatccccag cgcgacagcc agaatcgcca cccaattgac actcatcata
    24541 cgcgtggtcg caaagtgctc atagcggcga cggttcatca gataatcagc gatgatcacg
    24601 ccgcccaccg gaggaatagc tgccgaaagg aaggtcaacc agccgacaaa attgttatac
    24661 agccataatg cgcagaccgt accgataata ccgttaatca ccgaaagggt tttgctcgac
    24721 ataccggtaa tgttggcgaa acctaaaccc gacgcataga gcgcgttatc gttggtggtc
    24781 cagatattca gccccagcac cacaatcgca ggcagcagca ggccctgagc aatcatcaca
    24841 tcagagatat ccgccatccc cagtgccgcc gcgcccgctg caccgaaaat aaacatcaac
    24901 gagttgccga ggaaaaaggc caccatcgcc accagcaccg ccagtttggc attacgacca
    24961 aaccggacaa agtcagcggt gagcgtgccc gcactgataa atgaccccac aaccagcgcc
    25021 agcgcgacat tgaaatctaa cggttgtgcg ggaacgaccg cttttaatgc gtccaggccg
    25081 cccatgccgt taacggccag ccacacggaa taaccgccaa gacaggcgat agccggaacc
    25141 gcaatcaccg aaagaactgt cagcgccgaa atgccaaaga agacggtgac ggtcatcagt
    25201 aaaccggaga cggcaatcag caaattaata tccagcccgg ttgccttgcc caccggaata
    25261 gcaaacatcg ccacgccaac gccaaaccag ccaacctggg tgccgcccag cagcagtgaa
    25321 ggcaaccatg agcctttaac accaaacgag aagcgagcaa gaagatgggt ggtcaggccg
    25381 gtttttgcgc caatgtaacc aagaaatgaa gtgtaaatac cgaggagaag attaccgatg
    25441 agaactgcga ggaagaaatc atgatagcta agaccggttc cgagagtgcc gccggtccac
    25501 atactggcgg aaaagaaggt taatcccagc atgacgaacg tcaatgccaa tacccctttc
    25561 cgcgccgact gcgggactgg cccctggcta aagttgttat cttgcgacac gaaattcccc
    25621 ctttttgctg tttgaaaccc aaaaaatccg ccgcattcta ttcatctgaa gataaaaagc
    25681 aatcgttttc gtatggggat atatttctta tatgaatgag tacaaaaagg cgggggaaac
    25741 ttatctgtgt aaaaacagtg ggtaggcatg ataagacgcg tcagcgtcgc atcaggcatc
    25801 tgcgcatggt gtcggatgcg gcgcgagcgc cttatccgac ctacggttca ggcctgtagg
    25861 catgataaga cgcgtcagcg tcgcatcagg catctgcgca cggtgtcgga tgcggcgcga
    25921 gcgccttatc cgacctactc ttccatcgcc tggcggatct gatccaacga cgccggatca
    25981 tcaatggtcg tcagatcccc aggatcgcgt ccttcgcaaa tcgcctggat cgtgcggcgc
    26041 agcatttttc cggatcgcgt ttttggcaat tgcgagacaa accagacgtg cgccgggcgg
    26101 ccaaagttgc caatctggct gtccaccagc gccataatcg ccttctcttg cgagtgcgcc
    26161 acctcacggt cttccagact gtcgctctct ttcggaatga caaacgccac cgccacctgc
    26221 cctttcagcg catctttcac cccaaccacc gccacttcgg caacgcccgg atgactggag
    26281 atactctctt caatctcacg cgtacccagc cgatgtccgg caacgttaat cacatcgtca
    26341 gtgcgcccga gaataaagtg ataaccgtca gcatcgcgga tgccccagtc aaaagtggcg
    26401 tacaccggac gggaaaacag cgaccagtac gtcttcacaa agcggtcgtc gtcgccccag
    26461 atggtctgaa tacagcctgg cggcaatggc ccctccacta ccagcatccc tttctcattg
    26521 acgccacacg gttcgccggt gacttcattg agcaactgca cgttatagcc atacatcggc
    26581 acgccggggc ttcccagacg cgtcggtctg tcatccagac cgcgagcaat cgccataatc
    26641 ggccagccgg attcggtctg ccagtagttg tcgatgaccg gcacatccag cgtattgctc
    26701 acccaactgg cggtcggctc gtccagcggt tctccagcca gatagagcac ttccagcgac
    26761 gaaagatcgt gtttgcgaat ttcagcggta gggaattttt tcagcacgcg aatggcggtc
    26821 ggcgctgaga acatgcggct aacctgatat ttctcgacaa ttttccacca cacgccgcag
    26881 tccggccagg tcggcaatcc ttcgtaaacg atagtcgcca tccccgccag cagcggcgcg
    26941 taaacgatat acgaatgccc taccacccag ccgatatccg aagcacaaaa gaacacgccg
    27001 cccgctttgc cgccaaaaat ggtgtccatc gaggtcgcca gcgccaccgc atatccgccg
    27061 acatcacgct gcacaccttt aggtttgccg gtcgtgccgg aggtgtagag aatgcaggag
    27121 gtttcgttgg attccagcca tgccaccggc acccgcgcgc cgatgtgctg atggcgcaac
    27181 gacgcgaaat cgacatcccg cccgctaacg cgcgccattt tcgccagccc gcgatccacc
    27241 agtaaaacgt gacgcggctg atgctgtgcc tgacttatcg catcgtcgag caatttttta
    27301 tacggaatga ttttaccgcc gcgcgccccg gcatcagccg agacaatcag caccggttta
    27361 gcgtcatcaa ttcgcgccgc cacgctgtgc gaggcaaaac ccccaaacac caccgagtga
    27421 atagcaccaa tgcgcgcgca ggccagcagg gtaatatgcg cttcggcaat catcggcata
    27481 tacaccagca cccgatcgcc acgctgcacg cccagcgagc gcagcattga cgccaccgca
    27541 ttcacttcgt catgcagttg gcggaaggta aaggtacgct cttcctctgt ttccgaagag
    27601 acggcaatca atgccagcgc ctctggctgt ttctccagcc agcggtcgat agcgttgtga
    27661 cacaagttgg ttcggccttc acaaaaccaa cgggcaaacg gcgggttgct gtggtcgagc
    27721 gtttgcgtaa agggcgtctg ccagtcaata cgccgggcct gctcggccca gaactgctcc
    27781 ggttcgttaa tcgaacgctg ataaaattcg ctaaaagaca taatgctctc ctgttgaact
    27841 tacgccttac cgccgtttac ttaaatgacg tacaggtcga gatactcatt gaccggcatc
    27901 tgttccaggc gagttctgtc gagagaaacc tccagaatgc gctgctgctg gcgagtcggg
    27961 aactggcgcg cgagattgat tttgaattta tcgaccagct tcggaatgcc atcctgacgg
    28021 cggcgagcat gaccaattgg gtactccacc accacttctt caaatcgcgt gccgtcggtg
    28081 aactcaaggg ttatggcatt ggcgatggcg cgtttttccg ggtcgtggta gtcagcggta
    28141 aacaccggat cttcaaagca attgatcttc tcgcgcagag cgtcgatgcg tttatcttgc
    28201 gcaacgttgt cctcgtaatc tgccgccgtt aagcgcccga acagcagcgg gatcgccacc
    28261 atgtactgaa tgcagtggtc gcggtcagcc gggttattga gcggcccttt tttgtcgatg
    28321 atgcgaatac aggcttcgtg ggtgcgaatg gtcacttttt cgatatccgc cgccgttttg
    28381 cctgctgcct gcatctgttc atagagcgtc atcgctgctt caactgccgt ctgggagtgg
    28441 aactccgccg ggaaggagat tttgaacagc acattttcca tcacgtaaga accatacggg
    28501 cgctggaagc ggaacgattc gcctttaaag gagacgtcgt agaagcccca caccggcgca
    28561 gtcagggctg acggataacc catttcgccg gttttcgcca tcagtgccag acgtaccgcg
    28621 cgggaagtgg catcgcccgc cgcccaggat ttacgcgtac cggtgttcgg cgcatggcga
    28681 taggtgcgca gcgactgtcc gtctacccat gccagcgaaa cggcgttgag aatttcctcg
    28741 cgggtcaggc cgagcatttc ggcgaccacg gcggtggaag ccactttcac taacagaacg
    28801 tggtcgaggc cgacgcggtt aaaggagttt tccagcgcga tgcagccctg aatttcatgg
    28861 gctttgatca ttgcggtcag cacctgtttc atggtcaacg gcgctttgcc gctggcgacc
    28921 gcgttgcgcg aaagccagtc cgccgttgcc agaatgccgc cgaggttgtc ggaaggatgc
    28981 ccccactccg ccgccagcca ggtatcgttg aaatcgagcc aacggatcat cgcgccaatg
    29041 ttaaatgccg cctggacggg gtcgagctga aactgagttc cggggacgcg cacgccattg
    29101 ggtacgacgg tgccggggac aattggcccc agcagttttt tacaggccgg atattcgaga
    29161 gcttccagac cacagccgag cgtgtcgagc aggcagtaat gtgcggtgtc gtaggctact
    29221 ctggagctga tttcgtagtt catcacgtaa tcgacgatat caacgatttc acgatcaaat
    29281 tccgggcgaa tgttgttgat ttgagctgac attgggtacg tttccttatt gttattcgta
    29341 gaggtttact ggcgcttatc cagcgcgaca aactggcggt cttccggtcc aacataattg
    29401 gcggaaggac ggataatttt gttgtcctga cgttgttcga taatgtgcgc cgcccagcca
    29461 gtgacgcggg cgataacaaa cagtggtgtg aatatctcgg tgggaacacc catcatgttg
    29521 taggaaacag cggagaacca gtcgagattg gggaacatct ttttgctctc ccacatcact
    29581 gtttccaggc gatcggcgat gttgtacatc ttcagcgagc cgccttcctg cgagagctgc
    29641 tttgccacgc gtttgatcac ctggtggcgc gggtcggcga tggtataaac cggatgccca
    29701 aaaccaatga ccacttcttt gttttccacc cgcttacgga tatcagcttc ggcttcgtcc
    29761 ggcgtttcgt agcgttgctg gatctccagc gacacttcat tcgctccgcc gtgtttcggc
    29821 ccgcgcagtg cgccaatcgc gccaataatg gcggaataca tatcagagcc agtgcccgca
    29881 atcacccggc tggtaaaggt ggaagcgtta aactcgtgtt cggcgtacag caccagcgaa
    29941 atatgcatcg ccttttccca gctttgcgac ggcttttcgc cgtgcagcag atgcaggaag
    30001 tgaccgccga tagagtcgtc atcggtttcc ggctgaatgc gttcgccgtt gtggctgtag
    30061 tgataccagt agagaagaat cgagctaagt gacgccagca gtttgtcggc aatatcccgt
    30121 gcgccagaga cggtatgccc ctctttttct ggcagcgtgc agccgagcgc ggaaacgccg
    30181 gtgcgcataa catccatcgg gtgtgacgcc gccggtaagg cttccagcac ggtacgcacg
    30241 ttagccggta aaccacgcag ggctttcagt ttcgttttgt aggcggcgag ttcgtcacgg
    30301 gttggcagtt tgccgtggat cagcagatgc gccacttctt caaattcaca atgttccgcc
    30361 agatcaagaa tatcgtagcc acggtaatgc aggtcgttgc cgcttttacc cacggtgcag
    30421 agcgccgtat tgcccgccgg aacgccggaa agtgccaccg attttttcgg tttaatgaca
    30481 tgggtactgt tttgcaggat cgttgtgtcg ctcatattgt cctcgtcatt gttatcattt
    30541 gtagggtcga gattgttgtc ggatgcgacg ctatgcgtct tatccgacct acagttcagt
    30601 tcacctcgta ggcctgataa gtcgcgtcag cggcgcatca ggcatcggtt gtcgaataca
    30661 accaacgggc gtttttattt cacctgacca cgggcaaaca ggtcgtcgag cttctcttcg
    30721 tactggtagt agttgatgct ttcgtacagc tcgttgcggg tctgcatggt gtcgatgacg
    30781 cttttctgtg tgccttcctg acgcaggatg ttgtagacat gttcagcggc gcggttcatg
    30841 gcgcgaaacg ctgaaagcgg gtagagcgcc attgcgacat gggcgctgcg taattcgtcg
    30901 gtggtaaaca gcggtgttgc gccaaattcg gtaatgttgg cgaggatcgg tacctgcacc
    30961 gcatcggcaa actggcgata catggcgagt tcggtaatcg cctccgggaa cagcatttcg
    31021 gcacccgctt caacataggc ctgcgcacgc tcgatcgccg catccagccc ctctaccgcc
    31081 agcgcatcgg tgcgcgccat gatcacaaaa tcaggatcgg ttttcgcatc caccgccgca
    31141 cggatccgat ccaccatttc ctctttcgag acgatcgctt tgttcgggcg atgaccgcag
    31201 cgtttcgcac caacctgatc ttcaatatgc aatcctgccg caccggcttt aatcatcgat
    31261 ttcacggtgc gcgccacgtt aaaagcagaa gaaccaaaac cgatatccgc atccaccagc
    31321 agcggtagcg aacaaacgtc ggtgatacgg cgaatgtcag tcagcacatc atcaagagta
    31381 gaaataccga gatcgggcag ccccagcgac cctgccgcca cgccaccgcc ggagagataa
    31441 atcgcctgat atccggcacg ctgcgccagc agcgcatggt tagcgttgat agtgccaaca
    31501 atctgcaatg gattttcttt agtcagtgca gcgcgaaacg ctttacctgg agagtgtaga
    31561 gacatagccc atcctttgtt atcaacttgt tatttgcgtt gataaagaca aagcaatggg
    31621 tgtgccacaa ttgccttaat aaaattaaac atttaatttc aatgagttaa agataaattc
    31681 atgaagtaat ccgcaaatat gcgtttcagt taacgtttca ggcaatgttt caccgcgttt
    31741 cattgcaaca attatgaaac aagactaaac ccaatattcg gtttcttaac tttgcggtgc
    31801 gctatggcac atccaccacg gctgaatgac gacaaaccgg ttatctggac ggtatctgta
    31861 acgcgcctgt tcgagctgtt tcgcgatatc agcctcgagt ttgatcacct ggcgaatatc
    31921 acccctatcc agcttggctt tgaaaaagcg gtgacctaca tccgcaagaa actggccaac
    31981 gaacggtgcg acgccatcat cgctgctggc tctaacggcg catatcttaa aagtcgcctg
    32041 tcagtgccag ttattttgat taaaccgagc ggctacgatg tgttacaggc actggcaaaa
    32101 gccggaaaac tcacctcttc tatcggtgtt gtcacctatc aggaaaccat tccagcgctg
    32161 gtggcgtttc aaaagacctt taatttgcgc ctcgaccaac gtagctacat caccgaagaa
    32221 gacgcccgcg ggcaaatcaa tgagttgaaa gccaacggca ccgaagcggt ggtcggcgca
    32281 gggctgatta ccgatctggc agaagaagcc ggaatgaccg gaatttttat ctattccgcc
    32341 gccaccgtgc gccaggcatt cagcgatgcg ctggatatga cgcgcatgtc gttacgccat
    32401 aacactcacg atgccacccg caacgccctg cgtactcgtt acgtgctggg cgatatgctc
    32461 ggtcaatcac cacagatgga acaggtgcgg cagactattt tgctgtatgc ccgctccagt
    32521 gcggcggtgt tgattgaggg ggaaacgggg acgggcaaag agctggcggc ccaggcgatt
    32581 catcgggaat atttttcccg ccacgatgcg cgacagggca aaaagtcaca tccgtttgtt
    32641 gccgttaact gcggggcgat tgccgaatcg ctgctggaag cggagctgtt tggctatgag
    32701 gaaggggcgt ttaccggctc gcgacgcgga ggacgcgccg ggctgtttga aatcgcccac
    32761 ggtggtacgc tgtttcttga cgagataggc gaaatgccgc tgccgttgca gactcggctg
    32821 ctgcgggtgc tggaagaaaa agaggtcacc cgcgtcggcg ggcatcagcc tgttccggtg
    32881 gatgtgcggg tcattagcgc cactcactgc aatctggaag aagatatgca gcaagggcag
    32941 tttcgccgtg acctgtttta tcggctgagt attttgcgtc tacaactgcc gccgctacgc
    33001 gagcgggtgg cggatattct gccgctggcg gaaagctttt tgaaaatgtc tctggcggca
    33061 ctctctgtcc ctttttctgc cgcattacgt cagggattag agacctgtca gattctatta
    33121 ttgcactacg actggccggg caatattcgt gaactgcgca atatgatgga acgactggcg
    33181 ctgtttttaa gtgtggaatc gacgccggat ttaacgccgc aatttttgca gctgctactg
    33241 ccggaactgg cgcgcgagtc ggcgaagact cccattccag gcttgctgac agcacaacag
    33301 gcactggaga aatttaatgg cgataaaaca gcagcggcga attatttagg tatcagccgg
    33361 acgacgttct ggcggcggct gaaaagctga actgccgaat gcagcgcgaa cgctttatcc
    33421 ggcataaatt tcgatagatg atgatcaccg ataaactaac gcatcgggga tgtttacata
    33481 ggattcagaa ttacttcttc ttataaatat ttgccgtgcc gtggatctta ttgtcagttt
    33541 gaccagaggt cagtaccaac acatcagccc ctttttcatc ggcttttttg atcaaatctt
    33601 ctttcgcatc tgcggtcgac atttcattac tggttgaaat atcacctatt ttttcatatt
    33661 gcgatgcaac cttttcaaat tccgctttgg tcattaattc tgcggcatag acattggtaa
    33721 cggcaaacgc taacgcaccg actaacattt tagagattat tttcatatta cggctccaga
    33781 gttgttatca cactatcacc cacccacatt attgggttga ttaataatta gttcagtcag
    33841 ggcataaacg ccagtaaccg gaaataagac actt
//
LOCUS       DACADA010000034        33171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.034, whole genome shotgun sequence.
ACCESSION   DACADA010000034 DACADA010000000
VERSION     DACADA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.034"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(167..457)
                     /gene="ilvN"
                     /locus_tag="ISB17_001629"
     CDS             complement(167..457)
                     /gene="ilvN"
                     /locus_tag="ISB17_001629"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP1979573.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(461..2149)
                     /gene="ilvB"
                     /locus_tag="ISB17_001630"
     CDS             complement(461..2149)
                     /gene="ilvB"
                     /locus_tag="ISB17_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAP1979574.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(2255..2353)
                     /gene="ivbL"
                     /locus_tag="ISB17_001631"
     CDS             complement(2255..2353)
                     /gene="ivbL"
                     /locus_tag="ISB17_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAP1979575.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            2754..3938
                     /gene="emrD"
                     /locus_tag="ISB17_001632"
     CDS             2754..3938
                     /gene="emrD"
                     /locus_tag="ISB17_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAP1979576.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3946..4443)
                     /locus_tag="ISB17_001633"
     CDS             complement(3946..4443)
                     /locus_tag="ISB17_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP1979577.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(4440..4802)
                     /locus_tag="ISB17_001634"
     CDS             complement(4440..4802)
                     /locus_tag="ISB17_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAP1979578.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(4792..5139)
                     /locus_tag="ISB17_001635"
     CDS             complement(4792..5139)
                     /locus_tag="ISB17_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAP1979579.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(5199..6693)
                     /locus_tag="ISB17_001636"
                     /pseudo
     CDS             complement(5199..6693)
                     /locus_tag="ISB17_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(6690..8405)
                     /locus_tag="ISB17_001637"
     CDS             complement(6690..8405)
                     /locus_tag="ISB17_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP1979580.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            8572..9438
                     /locus_tag="ISB17_001638"
     CDS             8572..9438
                     /locus_tag="ISB17_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1979581.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(9528..11189)
                     /locus_tag="ISB17_001639"
     CDS             complement(9528..11189)
                     /locus_tag="ISB17_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAP1979582.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLHLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(11387..11815)
                     /gene="ibpB"
                     /locus_tag="ISB17_001640"
     CDS             complement(11387..11815)
                     /gene="ibpB"
                     /locus_tag="ISB17_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAP1979583.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(11927..12340)
                     /gene="ibpA"
                     /locus_tag="ISB17_001641"
     CDS             complement(11927..12340)
                     /gene="ibpA"
                     /locus_tag="ISB17_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAP1979584.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            12646..12978
                     /locus_tag="ISB17_001642"
     CDS             12646..12978
                     /locus_tag="ISB17_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP1979585.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(12982..14229)
                     /locus_tag="ISB17_001643"
     CDS             complement(12982..14229)
                     /locus_tag="ISB17_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAP1979586.1"
                     /translation="MAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPS
                     GASFTGETIERVNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYY
                     DFHHRRGVIVESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMH
                     ELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYE
                     EGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPR
                     GVVQRRLTFTHHRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEP
                     RQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVV
                     FSPDGQWLAWMEDGQLWITETDR"
     gene            14297..15397
                     /gene="cbrA"
                     /locus_tag="ISB17_001644"
     CDS             14297..15397
                     /gene="cbrA"
                     /locus_tag="ISB17_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAP1979587.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(15358..16695)
                     /locus_tag="ISB17_001645"
     CDS             complement(15358..16695)
                     /locus_tag="ISB17_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979588.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(16770..17918)
                     /gene="dgoD"
                     /locus_tag="ISB17_001646"
     CDS             complement(16770..17918)
                     /gene="dgoD"
                     /locus_tag="ISB17_001646"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP1979589.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(17915..18532)
                     /locus_tag="ISB17_001647"
     CDS             complement(17915..18532)
                     /locus_tag="ISB17_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP1979590.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(18516..19394)
                     /locus_tag="ISB17_001648"
     CDS             complement(18516..19394)
                     /locus_tag="ISB17_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAP1979591.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            complement(19391..20080)
                     /gene="dgoR"
                     /locus_tag="ISB17_001649"
     CDS             complement(19391..20080)
                     /gene="dgoR"
                     /locus_tag="ISB17_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAP1979592.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            20359..21015
                     /locus_tag="ISB17_001650"
     CDS             20359..21015
                     /locus_tag="ISB17_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979593.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(21059..21871)
                     /gene="yidA"
                     /locus_tag="ISB17_001651"
     CDS             complement(21059..21871)
                     /gene="yidA"
                     /locus_tag="ISB17_001651"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP1979594.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(21985..22392)
                     /locus_tag="ISB17_001652"
     CDS             complement(21985..22392)
                     /locus_tag="ISB17_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAP1979595.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(22527..24941)
                     /gene="gyrB"
                     /locus_tag="ISB17_001653"
     CDS             complement(22527..24941)
                     /gene="gyrB"
                     /locus_tag="ISB17_001653"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP1979596.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(24970..26043)
                     /gene="recF"
                     /locus_tag="ISB17_001654"
     CDS             complement(24970..26043)
                     /gene="recF"
                     /locus_tag="ISB17_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP1979597.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(26043..27143)
                     /gene="dnaN"
                     /locus_tag="ISB17_001655"
     CDS             complement(26043..27143)
                     /gene="dnaN"
                     /locus_tag="ISB17_001655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP1979598.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(27148..28551)
                     /gene="dnaA"
                     /locus_tag="ISB17_001656"
     CDS             complement(27148..28551)
                     /gene="dnaA"
                     /locus_tag="ISB17_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP1979599.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            28848..28928
                     /locus_tag="ISB17_001657"
     CDS             28848..28928
                     /locus_tag="ISB17_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979600.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            29158..29298
                     /gene="rpmH"
                     /locus_tag="ISB17_001658"
     CDS             29158..29298
                     /gene="rpmH"
                     /locus_tag="ISB17_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP1979601.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            29315..29674
                     /gene="rnpA"
                     /locus_tag="ISB17_001659"
     CDS             29315..29674
                     /gene="rnpA"
                     /locus_tag="ISB17_001659"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP1979602.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            29638..29895
                     /gene="yidD"
                     /locus_tag="ISB17_001660"
     CDS             29638..29895
                     /gene="yidD"
                     /locus_tag="ISB17_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP1979603.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            29898..31544
                     /gene="yidC"
                     /locus_tag="ISB17_001661"
     CDS             29898..31544
                     /gene="yidC"
                     /locus_tag="ISB17_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP1979604.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            31650..33014
                     /gene="mnmE"
                     /locus_tag="ISB17_001662"
     CDS             31650..33014
                     /gene="mnmE"
                     /locus_tag="ISB17_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP1979605.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         7964 a         8912 c         8459 g         7836 t
ORIGIN      
        1 ctatcaacac tgctccaggt atttggggcc gtgaagtgaa ttggcctgat gatatgaata
       61 agaaatcaat gttgtcttca atggacgaat atatactctg aataatttga gttgcagaat
      121 ataagctcgc ccggatgtaa atatcccatc caggcgagaa aatcacttac tgaaaaaaca
      181 ccgcaatctt gttaaacatc gtcgggtcgg actgattacg ctgcactttc accacatctt
      241 ccagcttgtc gatttggctt atcatctgct ccagacgctg gtcgtcattg accagtagcc
      301 agatgtggct tttgtcgctg tcctgaatcg gcaggcagag aatgccttca acgttaaaag
      361 cgcggcgggc aaaaaggcca caaacgtggg tcattacacc cggatgattg cgaacggtga
      421 gctccagaat cacgttatca tgagttgtgt tttgcatggc ttattccccc accatttcag
      481 tattcgccgc acctggcggc accatcgggt acactttttc ttcggcatca atgcgcacat
      541 ggatcagcgc cgggccaggg cgattgatga tttcctgcaa tgcagcctgc ggatcggctt
      601 cgttattcaa atcacaggtt tcgaggccga atccggcggc aatctgcata aagttgattt
      661 tgcccggata ggtagcggca aaaacgccct gcttgtagaa cagactctgt tgctgatgca
      721 ccagccccag cgcttcgttg ttcatcaaaa tgattttaac atccagctga ttttcactgg
      781 cggtcgccat ctcctgaata ttcatcatca ggctgccgtc gccggagaag cacaacactt
      841 tgcggtccgg gttcgccagc gccgcgccaa tcgccgcagg caggccaaaa cccatcgtgc
      901 ccagcccgcc ggaggtcagc cactggcgtg ggcgattgag cggataggct tgcgcggtcc
      961 acatctgatg ctgacccacg tcggtagtga taatcgcatt gtcatcgaca caggcggcaa
     1021 cggcgttgat caggccgtaa tggcttaatg gatcgcacgc tttcgggatt ggacacggga
     1081 attcacgctg caaatccgcc accaactggt gccactctgc acgcggttgc gcttccacca
     1141 gcgggatcaa ctgcgccagc acgtcatcaa catccgcctg aattgccaca tgcggttgct
     1201 tgattttgcc cagctctgcg cggtcgatat cgacatgaat aattttggca ttcggacaga
     1261 actgctctgt tttgccaatc gcccggtcat caaaacgcgc accgagtaca atcagcagat
     1321 ccgcctcctg caaaatatag ttggtgctgc gcacaccgtg catccccagc atacccagcg
     1381 acagcggatg cgcttttggc aacataccca gcgccattaa agtcatggtg gtaggcagtt
     1441 gcgctttctc cgccagttca cgcactcgcg caggcgcgtt gatcacacca ccacccagat
     1501 aaagcaccgg gtgtttggcg gcgttaatca tcgccgccgc gtcacgaata ctttcttcgc
     1561 taaaggcggg tgcggcggct ttttctgcca tagcgggctg tgtttcaatc tcaaaaactg
     1621 ccgtttgcac atccttagga atgtctatcc acaccgggcc agggcggcct gattgcgcaa
     1681 tgctgaaggc atcgctcatg acctgcggga gttcttcgat atgtctgacc agatagttgt
     1741 gtttggtgat ggggatagag atgccgtagg tgtcgacttc ctggaaggcg tcggtgccga
     1801 tcatcgaggc ggaaacctga ccagtgatgc aaatcagcgg gatggagtcc agcctcgcat
     1861 cggcaatggc ggtcaccagg ttagtcgcac ccggtccgct acaggccata cagaccgccg
     1921 gtttaccgtc ggtgcgcgcc attccctggg cgataaaccc cgccccttgt tcgtggcgag
     1981 ccagaatatg gcggatttgc gtgctttggc ttaaggcatc gtaaacaggc aggatagaac
     2041 cgcccggaat acccgtcaca atcttaatgc cctgctgttc caggaaatga acgataaatt
     2101 ctgcgccggt aaagcgctta cgcgtcgatg ttgtgcccga acttgccatg ctccagtcct
     2161 tttcttctgg gccgactttc cgggaggtgc ttaaacgaaa aaccccgccc ggtttgcgcc
     2221 ggcggggttt tggaatcgtg tgttgttcca gtccctacgg cgcattgccg acgaccacca
     2281 ccacacgcac gacgaccact gcggcggatg gcgcagttgg tagtagtttt gcgttgaaca
     2341 tggaagtagt cattagggac cttgttggtt ttgtgtttaa caatatttat acaagcacag
     2401 ctttacaggg gagacaatgg aaaatttttc ttccggataa aaatacgtgg cagatcacat
     2461 tttgaactga aactcaggca ataaagcatc atcacctgaa agacggtcag gttgttgaca
     2521 gagtgcgcct tattcatgtc gaatacagcg taaacacttt actctaccaa ccgcaccacc
     2581 actgtttccg gcaacattta ttgccgcttt tgtttacata ttctgccgct aaacaattcc
     2641 ccattcctgg cgtatatctg gctaacattc atcaatgtga tagattcctc tcccgcattt
     2701 atgggaatgc gtagtgactt attctaatta tttttataaa agcatccgtg ataatgaaaa
     2761 ggcatagaaa cgtcaatttg ttattgatgt tggtattact cgtggccgtc ggtcagatgg
     2821 cgcaaaccat ttatattcca gctattgccg atatggcgcg cgatctcaat gtccgtgaag
     2881 gggcggtgca gagcgtaatg ggcgcttatc tgctgactta cggtgtctca cagctgtttt
     2941 atggcccgat ttccgaccgc gtgggtcgcc gaccggtgat cctcgtcgga atgtccattt
     3001 ttatgctggc aacgctggtc gcggtctcga cctccagttt gatggtattg attgccgcca
     3061 gcgcgatgca ggggatgggc accggcgttg gcggcgtaat ggcgcgtact ttgccgcgcg
     3121 atttatatga acggacacag ttgcgccacg ctaacagcct gttaaacatg gggattcttg
     3181 tcagtccgct gctcgcaccg ctaatcggtg gtctgctgga tacgatgtgg aactggcgcg
     3241 cctgttatct ctttttgttg gttctttgtg ccggtgtgac tttcagtatg gcccgctgga
     3301 tgccggaaac gcgtccggtc gacgcaccgc gcacgcgcct gcttaccagt tataaaacgc
     3361 ttttcggtaa cagcggtttt aactgctatt tgctgatgct gattggcggt ctggccggaa
     3421 ttgccgcctt tgaagcctgc tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta
     3481 tgacggtcag tattttgttt attctgccga ttccggcggc attttttggc gcatggtttg
     3541 ccggacgtcc caataaacgc ttctcaacgt tgatgtggca gtcggttatc tgttgcctgc
     3601 tggctggctt gctgatgtgg atccccgact ggtttggcgt gatgaatgtc tggacgctgc
     3661 tcattcccgc cgcgctgttc tttttcggtg ccgggatgct gtttccactg gctaccagcg
     3721 gcgcgatgga gccgttcccc ttcctggcgg gcaccgctgg cgcgctggtc ggcggtctgc
     3781 aaaacattgg ttccggcgtg ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg
     3841 gcagcctggg attgttgatg accttaatgg gattgttgat cgtgctgtgc tggctaccgc
     3901 tggcgacgcg gatgtcgcat caggggcagc ctgtttaagc tcacgtcacc gcagcatcgt
     3961 catcagctcc atgggagaac gatgctgctt tatcagatca cgcatcaccc gcatatgcgg
     4021 cgcggagtaa gaataaaacg cctgatagcc cgcacaaagc acgttgtctg gtttatcttc
     4081 ccgagcgtgc gggcacaggc gatgccagtc gcacgcctgg cagtcacggc gcggttgcga
     4141 cttttcgctg ccacaccatt tttgtaaggt ggtgtcaaaa agttcaatcg aaatacgact
     4201 gatatctgcc cgcacccaac actcgaacag ggcaattaaa aatttccccc acagctctgc
     4261 tgtcaccgaa gcggccgtca gatcgccccg gtcatcaaat tcaagatggg gaataagctg
     4321 tactgagcgg atgcccagct gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc
     4381 cttatactct acaaccagtt tatgcctgtc ctcttcagca tcaatgacct gacttcctgt
     4441 catgcgactc tctcaataaa tacgataagt tgatgtatat gcgttacagc aaacagtatt
     4501 gcgagagata acaccgcgag ggagatcaaa aatttgtcac ggactacgtg aaattgggaa
     4561 aaatcgctat tcgtgacatc cattaaattg cgattacggg tgtagtgcca gaggatcagc
     4621 gccacaatgg cgaggatgcc aatcgaaatc cagaataaca tgcccgcctg gtgccagttg
     4681 tgtttgatag ccaacgccat caacgcgccg tagcccagca tggtacgaaa ccacgccagt
     4741 gatgtgcgtt ccggctgcag ccctgggtcg gcaatgcgtc tggctttgcg gctatccggc
     4801 atacaacacc agtcccatga cgatcactgc cacgaccatc aatattaagc tgatgatgaa
     4861 taagctgttg gtatagggca ggtcctcttt caggcgcatc gctttttcat tgcgtaacca
     4921 gcgcaagtag ccatacatcg ctaacccgcc ggaaaacaga cacaacaaca acgccagcag
     4981 ttcgcggatg acgggcgtgg cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc
     5041 cagaaatcct agtgcagtac ggatccacgc cagaaaagta cgctcattag ccagcgagaa
     5101 gcggtaatcg ggcgcttctc cgaggcggga aattttcatt gttgctcctt gtaaactgtt
     5161 cctcgccgaa aaattaggcc aggaacagca ataatgcatc agaacttctg ttttttctct
     5221 tccaccttca ccccttgtgt cggtaaaccg gtgtcatagt cgcgtaccac tggagaataa
     5281 ccatcctgtg ggcgtggacg aaacgccccc atccagcgcg gttgtgcatc tttgcgccac
     5341 ggacgcaggt tccattgata actgcggaac ggatcgcgaa ttttgtccat gtaatccaat
     5401 aaggcgtcat gcattttgct gcgaacgtcg gcaaaatgga tatcatcgat caggttatgc
     5461 atttcatttg ggtcattacg gcgatcgtaa agttcatcac tggtgaagag gttgagcacc
     5521 agtttaaagt catccgttac ccagcaacgc accggaataa aaccgccaaa gctgtcatgc
     5581 tcaatctcgt agcgattaaa ttccaccaac acgccacgtg gttcttttac ggcaaggata
     5641 ttttcccccg gcagaatctc tggtttttca atatctgcca gcgccatcat tgtcggcagt
     5701 aagtcaatat gactgactgg cgtatcgacc tgtcgccgct ccccttgcgg cgaacggatg
     5761 atcagcggaa tgcgggtgat gtcgtcatac atcgccgctc ctttactgat cagcttatgt
     5821 gcgcccatca tttcgccgtg atcggaggta taaataaccc acgtattttc acgttgctct
     5881 ggcgttaagg cgttgatgac ccgtccgatt tggtcatcaa caaagtcatt gcaggcaaaa
     5941 tagagcggat ggtgataaag cccgtcatca ccgactggcg atggcatcgc ctgcgcccat
     6001 aagcggtgat gttccggttt gttcgccagg tcatcctctg ctttctcacc cagttcgtag
     6061 taaaaatcag tgtatttctc taaatactcc accggacagg tgaacgggtg atgcggctca
     6121 tcatacgaaa tcaccatcag gaaaggttcg tcggcgcgcg cgggctgttg cagaaaatcc
     6181 accgcccgat tgctgatgcg gtgcgcccag gtgaaggttt cgtcaatatg gttcgcctgt
     6241 aaatcttcga cgctgtttag gccattgcgc cacaggctaa tctctttttc cgtcagttca
     6301 ctaagatagt tcgccccatc gaaccagtaa tcagcgtccc actccggcgg acactcgcca
     6361 ggtgccgaaa tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc
     6421 ggcatcctta aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt
     6481 ccacgggccg gactggttag cgtagatacc ggtgaatagt ccggcgcgtg caggcgtaca
     6541 aaccggtgaa caggtgtagg cggagttaaa gcgaatacct tccgccgcca gactatcaat
     6601 attttgcgta ttcagcggct taccgctata gcaaccgacc atattggtgg cctgggtatc
     6661 ggtcatgatg aacagaaaat tggggcgttt cataggctat cctttgcatc gggagtaaag
     6721 gttacggctg ggtcgtgacg atgccgccag ctgtcaaaaa tcaggtagac aatcactacg
     6781 gcggcaatga aataactgat catcgctaac cagcgcgtac cgtagccgcc gaattcagcc
     6841 agcccggcat agacgccaat catcgcgaac aggataccaa ttgacgcagt cttgacgttt
     6901 ttccacggtt tcatgtccac cgcaaacgca tctttgaagg tgaacggcgt ggcgcgcggt
     6961 ttgataaaac cgatcaccag catcacgacc acgttgatgc agaacgtaca ggccagcaca
     7021 tagaggaaat ggaagtcgaa cttcaccaga tagttgatgg tgatgtagct gattatgcca
     7081 atccccatcg ccacttttgc cgccagcgcc ggaatgcgcg ggaagaaaaa gcccataatg
     7141 atgatggtaa ccagcggcac gttgtaaata ccgttgagct gtttcatcca gctatacagc
     7201 ccctgcggcg cgttggcgat ccacggcgcg accatcaccg aaacgatggc gataaagaaa
     7261 ccaaatttgc gcccgacggt gaccagctgt tgcggctcgg cattctggtt aatgatacgc
     7321 cggtaaatac ccatactgaa taacgtactg gcgctattca gaaagccgtt gaaggtactg
     7381 atcaccgcac caaataacac cgcaccgaag aagcccacca gtggcaccgg cagaacgtta
     7441 ttgaccagcg tcgggtaggc catgtcggct ttcggtaaat cctgatacag atgaaacgca
     7501 atcaaccccg gcagcaccag taccagcggg tcgagcattt tcagcaccgc cgttaacagt
     7561 gccccctttt gcccttccgc caggcttttt gacgccagcg tgcgttgcac gatgccctga
     7621 ttggtacacc agtaaaaggt gttaaccaga atcaaaccgg taaatgccgc gccaatcggc
     7681 aagggatcgg tcgggccacc gactgagttt aatttctcgg cgtgaacggt ggtaagttgc
     7741 tcaatgccct gcataaagct gcccttgccc atcgcgatca ggccaaatac cggcaccatc
     7801 aacccgccaa taaccagccc aataccattg atggagtccg ccactgccat tgcgcgcagt
     7861 ccgccgatca ccgcatacaa aattcccgcc agaccaagca aaataaccag taaccagata
     7921 gccgcaccat cggaaagttg tagcgactca cccacatgaa acaggctgtt gagcgccaac
     7981 gcgccggaat agagaacaat cggcagaaag cagacgccgg tggcaatgag gaagcagaag
     8041 tcgatgataa tacgcgtcgt tttatcataa cgttcctcca gaaaatcggg gatggtggca
     8101 atgccgcgct tcagatagcg cggtaaaaag attagcgcga ggaagatcag cgtcactgca
     8161 gaggtgactt cccagcccat caccgacatg ccgcttttgt aggcctgccc ggaaaggccg
     8221 accagttgtt ccgtggaaag gttggttagc attaacgaag cggcaataac cggcgctttt
     8281 agtgaacgtc cggcaagaaa atagccttgt tgcgatccgg tatctgtttt acggaccttc
     8341 caccaggtga tgaccgccac cagcagcgta aaaccgacaa aactcaagat ttgtaacgaa
     8401 ttcatctcat agcccttatt tatgtttatg tcccggacgc atgaatatag ggagaggtaa
     8461 aatgcgtcgg gtcaggcagt cagattctta aaatacaaac gtcgtatccc tgaacggatt
     8521 gttttctgtt aagttcaggt tgtgagcata attcgccgga gggatattaa aatgaatgga
     8581 aaattgcaaa gttcggatgt aaaaaacgaa actccgtaca atattccctt attgattaac
     8641 gaaaatgtga tctccagcgg aatttctctg atatcgctct ggcataccta cgccgacgag
     8701 cattatcggg tgatctggcc gcgggacaag aagaaaccgc ttatcgccaa ctcatgggtt
     8761 gcggtgtata ccgtacaagg atgtgggaaa attcttttaa agaatgacga acaaataact
     8821 ctgcatggca actgtattat atttttaaag ccaatggata ttcactctta tcactgtgaa
     8881 ggtttggtct gggaacagta ctggatggaa tttaccccca ccagtatgat ggatattccc
     8941 gtcggtcagc aaagcgttat ttataatggc gaagtttata atcaggaact taccgaagtt
     9001 gctgagttaa ttacttcacc agaagcaata aaaaataatc tggcagtcgc ttttctgacg
     9061 aaaattattt atcagtggat ttgtcttatg cacgcagtcg gtaaaaaaga tccacaacgg
     9121 cggcaaattg aaaaattaat tgccacttta catgccagtc tacaacaacg ctggagcgta
     9181 gctgatatgg ctgccacgat cccctgtagc gaggcctggt tgcgtcgtct gtttttacgc
     9241 tataccggca agacgccgaa agaatattac ctcgatgcgc gtctggatct ggcgctatcg
     9301 ctattaaagc aacaagggaa ctcggttggc gaagtcgctg atacgctcaa cttcttcgac
     9361 tcctttcatt tcagcaaagc ctttaagcat aaatttggtt atgcgccgtc agctgtgctg
     9421 aagaatacgg acaggtaata accagaatgg cgagtgtgta aatgactcga tgatgttcag
     9481 aaataccagg cggagtacca tttacggcga ctccaccggg aaaagagtta accgatactc
     9541 cagaagagca ccgccagtaa ttggggtgtg ataatgcgca ggaacatcac taacggatag
     9601 acagtggcgt aagagagcgc cgctgcaccg ctggttggat gaagattatt agcaaacgcc
     9661 agcgccggag gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt
     9721 ttggctaaca tccgcgccag aataccaaca gtaatcagcg gaacggcggt gatcagggca
     9781 ccataaccaa tccagcttag cccttcgcca tccaccagtg tatggataaa atcgccgccg
     9841 gatttcaggc caacgaccga gaggaacagt acgatcccca gctcccgcag cgcgaggttg
     9901 gcgcttggcg gcataaacca gtacagcttg ccgatactgc cgatacgccc gagaatcaac
     9961 gccataatca gcggaccgcc cgccagcccc agtttcaacg cggccggaaa tcccggcaca
    10021 aagacgggaa tagaacctaa caatacgcca agcccgatac caataaacac tggcagcatc
    10081 tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg
    10141 gacggacgcc ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gctacttgca
    10201 actaattcaa caccagcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag
    10261 tgcaggtcgc gaatacgttt accgagcacg ttttcattgg tgaccaccac acgctcgacg
    10321 cgcaaatcag tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc
    10381 gcattatgta aatccgcagg ctgccccacc agatgcagca aatcgcccag ttgaataacg
    10441 gtttctggcg aaggcacttt tagggtttct tctcgtttca atcgtgagca aataacttta
    10501 tcgccattga gaatcggcac atctttaatg gctaaattat gcaaattagg attctcaact
    10561 cggatgttaa tcgtcctgat tagcgcaccg ccgttggttc gcgtggactc gtgctgctgg
    10621 gcctcagtct caacattgac gcggaaaata acccgcagca tccacatagt gaacaaaatg
    10681 ccgcaaatcc caaatggata cgccatcgca tagctcatcc ccatttgatc gaccatcgcc
    10741 atcggtgtcc ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtg
    10801 accgcgccgg aaaaaatccc cagcacgacc ggcagtggaa tatcaaacag tttatgcagg
    10861 atggcggtaa ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggtgtaat
    10921 ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg
    10981 aacaggatca ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc
    11041 gcctgagaaa caaaatggcc gacgatgatc ccgccgaaca gcacgccgcc aatgcccaat
    11101 ccaacaccgc gaaacttgac gttgccgata aacaacccga cgaccgccac caaagccaga
    11161 atactgaccg ttaatgctat atcactcatc gtccatttct cttgaaataa cattttaatt
    11221 gctacggatt atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg
    11281 aaagaacgtg ccgaaatatc ttaaacaggc gaaatttaac gcattgttga agaaaaaaga
    11341 caaaaaaaaa gccccaccag aatggcgggg caaagagaat agctagttag ctatttaacg
    11401 cgggacgttc gctgatagcg atacgctgcg ctgcgatggg ttcaggctca ttacgaatta
    11461 aatcaatatg cagtaaaccg tttacgaagg ttgcgccaga gacttccata ttttcagcca
    11521 gcgtaaagct caggctaaaa ggctgattca taagcccttg atgcagccat tttttctctt
    11581 cttttggctg ctccggtgtg ccttttacgc tcaggcgcgt accttccagt tgaatctcta
    11641 aatcttcctg acgaaaacct gccagcgcga gggtaatgcg gtagtggtta tcgtcgcttt
    11701 tctcaatgtt gtacggcggg aagctctggc tttcaccggc gttttgcagt gcgttggcca
    11761 gtttgtcaaa accgatccat tgacgcatca gtggggataa atcgaagtta cgcatagtca
    11821 tttctccttc taagaagcga gtaagtacct gcaaatccga agattcgcat atgctccctg
    11881 acggcgagca tggagatgtc aggccgcgcc aggcggcctt agggaattag ttgatttcga
    11941 tacggcgcgg ttttttcgct tccggaatca cgcgttcgag atcgatatac agcagaccat
    12001 tcaccaggtt agcaccacga acatgaatgt tctcagctaa ctggaattta cgttcaaagt
    12061 tgcgttcagc gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg gcgtgagcac
    12121 ctttcaccac cagcagatta tcctgggcgg taatttccag ttcgctctca gcaaaaccag
    12181 ccacagcgat agcaatgcgg taatggtttt cgtctaccag ttcaacgtta tacggagggt
    12241 agccgccatt actctggctc tggttgtttt ctaagtggtt aaacaaacgg tcaaatccaa
    12301 tagcagaacg gtaaagcggg gataaatcaa agttacgcat aatcaatagc tcctgaaatc
    12361 agcgagaatc taagaccttc cacaatggac aggtcaggta gccagaacac ccatcaggcg
    12421 cgtcctcatc ggctacgatg taaaaatggg tctggaaatg aacttttcaa gccttatcag
    12481 cggacttttt tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt
    12541 aaagtgcgat agccgccaca gtgcgacgta atgatgatgt tattttcagc aagggatcgc
    12601 tataactcat caggccaaca cccacgatca acagatgaat ttattatgat aaaaaatgtg
    12661 ttgttagcgt tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg
    12721 tcccataccg gggggaaaga aggaacgtat cctggcaccc gcgccagcgc aaccatgatt
    12781 ggcgacgatg aaaccaactg gggcacaaaa tcgctggcga tactcgacat gccatttacc
    12841 gcagtactgg acacaatatt gctaccgtgg gatgtgttcc gcaaagacag ctcagtgcgc
    12901 tcgcgcgttg aaaaaagcga agccgacgcg caagaaacca ataacgtcat tccccccgcc
    12961 aaaatgcctg caaactgaga gctagcggtc agtttcggtg atccacaact gaccgtcctc
    13021 catccacgcc aaccattgac catccggcga gaaaaccacg gcgtccgcag aaggtggatt
    13081 ggcgtggttt tcggttaaat actcaacctc gccgctttgc gcatgggcac aagcaatccg
    13141 attatccagc acaaatccca gccattcgcc tgatggatgc cagttaaatg cagactgaat
    13201 atccgtttta ttatgggtta actggcgtgg ttcaccgccc tgtggcgaga taagccacag
    13261 ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct gcggattaca
    13321 gcgcacccag tggcgcggga cgttgactaa tcccggagaa gcccgatggt gcgtaaaggt
    13381 taaacgtcgc tgcacgacac cacgcggtgg cgcgggcagg gttgtttccg ttccacttaa
    13441 cggcgcatca cctgccactt tccagccagc ttcatcttgc ggcaactcga caataaacag
    13501 ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt
    13561 tcctacccat ccttcttcat aagcacgatt gatttcatca ctgccaggct gtggcgtggg
    13621 cgtggtttta cttaccagca cgcaccagtg gctaccgctg tattcacgcg gatgctgctt
    13681 ttgtacgttg accgggccaa acggtgcagc aacaccgaca tttcgcaaat ccagcgccgg
    13741 atcgagttca tgcattacat ggtcgttgta ggtaaagctc accctttcac cgtttgggct
    13801 aaagacatgt acatgactgc cgccgcgcag cgctcctggc gtatatggcg cggtaatatc
    13861 cattgcatcg agattgctca ccttgccgct ttcaacaatc actccgcgcc gatgatgaaa
    13921 gtcgtaatac catgtttcat caggattttc cgggccgtgg atgaagacat acttctctga
    13981 cttcggatga acggtcacca cgccgacgta tgcgccctgt gacgcgcgat agataacctc
    14041 gacctcgccg gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgccccaga
    14101 aggccgcacg tcaaacacca gccactggct gtcgggcgtc caggtattgg tattggtgag
    14161 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggt
    14221 tcccgccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
    14281 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
    14341 gtcggcgttg gcgcgaaagt tagcaggcaa aatgcaggtg atcgcgctgg ataaaaagca
    14401 ccagtgtggt actgaaggtt tcagcaaacc ttgtggcggt cttctggcac cggacgcgca
    14461 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtcatcgcca acccgcagat
    14521 tttcagcgtc aaaaccgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
    14581 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt
    14641 tgaggtttac cacgacagcc tgtgtcgaaa aatctggcgt gaggatgata aatggcatgt
    14701 catttttcgc gcagacggct gggagcagca tattaccgcc cgctatctgg tcggtgccga
    14761 tggtgccaac tcgatggtgc ggcgacatct ctacccggat catcagattc gtaaatatgt
    14821 cgccatccag cagtggttcg cagagaaaca tccggtaccg ttctactcct gcatctttga
    14881 taatgcgata actgactgtt actcatggag tatcagcaaa gacggttatt ttatctttgg
    14941 cggtgcctat cccatgaaag acggtcagac gcgtttcacg acgctaaaag agaaaatgag
    15001 cgcctttcag ttccagtttg gtaaggcggt gaaaagcgaa aaatgcacgg tgctgtttcc
    15061 ctcacgctgg caggattttg tctgcggcaa ggacaacgcc tttctgattg gcgaagcggc
    15121 gggatttatc agcgccagct cgctggaggg gattagctat gcgctggata gcgcagagat
    15181 tctgcgttcg gtattactga agcagccaga gaagctcaat accgcttact ggcgcgccac
    15241 tcgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
    15301 ggctttaaga aagtggatta tgcgcagtgg agtggcacat attccacagt tgaaagatta
    15361 tccaacgcgc ttcacatcgc ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
    15421 ggcggagata taaaccagtg caggcgcgaa accgtaaccc tgcgccaggt accccaccac
    15481 cagcggaacg gtgatgccgc ccagaccacc ggcaaagtta aacacgccgc cggttaaacc
    15541 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gttatcgaag caaaaccgtt
    15601 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttcgc
    15661 acccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
    15721 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccaat
    15781 aaacgccgcg aggaatggca ccgtggtcat aaaacctgct ttcagcgccg tgattccttt
    15841 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgtag aagccaccgc
    15901 aaattgccca agatagacac cgatcagttt acgatggaac accagtttcc agtctttggc
    15961 tgttaacggc tgacgcgcct cttttttcac cggcgcatcg ccatccacca gaccgccgcc
    16021 atcacgaatg taatccagtt cagctttgct gatgcctttg gtcagacgcg gcggctgata
    16081 aaccttaaac caaatcagcg accagataat gccgatacca ccggtgacaa tgaacaccca
    16141 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcagaaacg ccagaccgac
    16201 aaactgacca gacgtataaa aaccaacggc agaagcgcgt tcatgttccg ggaaccaact
    16261 ggttaccatc cggttattgg tcgggaacgc gggcgcttcg aaaataccgg ttatcgcgcg
    16321 cagaccaatt aatgacatta agcccgtggc aaagccctgg aataacgtcg ccactgacca
    16381 gccaaatatc gcaataaaat aagtcacgcg tgaacctacg cgatctaaaa accaaccgcc
    16441 ggggatctga catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttgcgc
    16501 tttagtaata ccaaactctt cctgaatatg ggcagaagcc acggccaggt tagcacggtc
    16561 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
    16621 cggctttgct gcattaacgg gaatatccat agcgagcttt ctcctgattt tgggcatagc
    16681 gaagccgctc accatgcact gtaatttaca gagggttata tttttgtatt gctgttttag
    16741 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
    16801 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
    16861 tcgatttcca cgcctaagcc cggtttcgtt aatggtttaa agaaaccgcc gaccatgctg
    16921 aagtcctctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
    16981 atactttgtt cctgaaggac ggcgttatag gaaacaaagt cgatatgcag gcaagccgcc
    17041 agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca cgtcataggc ttctgccatt
    17101 ccggcaattt tgtagcattc ggtaataccg cctgcgtggg agagatccgg ttgcagaatc
    17161 gaaataccgc ctgcctccag cacacgttta aaatcgaagc gtgagaacat gcgctcaccc
    17221 gccgccagtg gaatatgcgt ttgtgccgcc agtttcgggt agtattcggc ctgttccgcc
    17281 agcaccggct cttcaataaa cagcggacga tacggttcca gctctttaat cagcactttc
    17341 gccatcggcg cgctgacgcg accatggaag tccagaccaa actcaatctg attgccaaaa
    17401 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
    17461 agccccagtt cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgtagcgtt
    17521 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
    17581 ttgtcgcgaa ccaggccgcc catcagttgc cagaccggcg cattcagcac tttgcctttg
    17641 atatcccata aagcctggtc gatcccggcg atggcactca tcagaatcgg accgccacga
    17701 taaaatccgg cgcgatacat cacttgccat aagtcgttga tgcgtgacgg gtcctgacca
    17761 atcaaatagt cactcagctc gtggactgcc gcttccaccg tgcgagcgcg accttcaatc
    17821 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    17881 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    17941 taacaaatgc tgctgcctgc tgcgcagtcc gttctacgga ctgcccggcg cgatagagat
    18001 cgctacctaa gcccgctccg gcacagcctg catctatcca ctgcgccagg ttttctggcg
    18061 tcacaccgcc aacggcaaag accgcgatgt tcggtggtaa taccgctttt aatgctttga
    18121 tgtattgcgg accaaaagcc gatgacggaa atattttcag tgcctgcgca cccgcgtcga
    18181 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca gacggtcatg ccgtagccca
    18241 ccgcacggcg gatcacttca ctatggatat tgggcgtaac gatgagctga cagcccattc
    18301 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    18361 cgccatacgc atcaacgatg gcgggaatgc tctgctccca ttgtggagaa ttcagcggga
    18421 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    18481 gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt ttgccactgc atgagcgatg
    18541 ttccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgtcacgtc acaacccatc
    18601 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatgact
    18661 tgttgatggg ccacatagtc gcgcatactg gcgacctctg cgccaatcaa caaaccagag
    18721 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    18781 aaaagctgtg gcaatacggc gggcgcatta aggccacgct caagaccagc cgcgaaagca
    18841 tcgacagcgt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    18901 tgatgtaatt caccggtcat cacggtgcga aaatcgttaa tttgctggct atcggcctgc
    18961 acccatttgc aatgcgtacc gggcatgaca taaagagagg aaggagcgag agtgcgcgcg
    19021 ccgatcaatt gtgtttcttc gccacgcatc acattgtggt tatcgtcatg agagacgcat
    19081 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    19141 aaacgggcag gaatggataa atacggcgca actttccagc cgacattgct gccgaccatt
    19201 cctgccatta ccactggcgt attctcttca cgccagtcgg tcgtcacttc tgctaacacc
    19261 gcagccggag attttccatt caggcgcgtg acgcctgctt ctgattgcct gctgtccagg
    19321 cagtggtcgc cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg
    19381 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg
    19441 ccgcctgctc tgccgcatcg ccgtcctgat gccttatcgc atcgaacagc gccttatgtt
    19501 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    19561 gctgcagcga actgatcgca atgctcagtt gttgtaacac cgggttatgc accgactgca
    19621 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttag
    19681 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaggtc gcgcgctctg
    19741 ctgcccaacg ggcaatagcc ggctctacca ggtttcgcac ttcgctcatt gcactgataa
    19801 gccgtgggtc atagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    19861 actggttacg cggagccaca aacgccccgc gataacgttt catttcaatc agccgcttcg
    19921 ccatcagcga acgaaatacc tcacggatga tgttgcgcga ggttgcaaac tcctcacaga
    19981 gttctgcctc agccggaagc ggcgagcctg gcacgtattt gccgtgaacg atctgtttac
    20041 ccagcgtaat aacgatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    20101 atgttcctct ctactttacc ccgatcgatg cataacgcgg caattttgta gtaccagcgt
    20161 gatgacgttc gcgtttgctg tgcgcataat gtagtacaaa tttatattgt tgtactacaa
    20221 tttagatcac aaaaagaaca atccataaga aaatgttcta cgtcgggcaa aaatctgaaa
    20281 agggagatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagcactc
    20341 ttgtttaaag gattaatcat gaaattgaat tttaaaggat tttttaaggc tgccggtttg
    20401 tgttctttgg catttgcgtt gaccggatgt atcacctggg gattggtttc caatactgca
    20461 agccgtccac atgccgagca atggcggtca gacacgatta agggtttatc gctggctgaa
    20521 gacagtaacg gtacgaaagg ttatgttttt gttggtgaat cgttggacta tctattgacc
    20581 accggcggcg atgaggtggt taacatgctg aacgatccgg caatccatgg tgaaagaatc
    20641 accgtttctg ataacgcgaa atttatttta agcagcagta ataagaattt ctctggtgct
    20701 attacgctct attacgactg gaacaacgaa gaagataagg cactggcaac acaatatggt
    20761 tttatttgtg atacccggcg ttgtacatgg atgctggacg gtttgaaagg ctcaatccac
    20821 cagaaaaata aaaaagcgga ttactcaaac gtgatggttt tccatcaacc ctttacggtg
    20881 ggattttatg agtacaaagc aacagatggc gtcccgcgcg gcctcgttaa tgcgctgctg
    20941 ccagtcactc ttacgctgga tatagtaacg tcaccactcc agttcctgat tttatgcacg
    21001 accagaaatt gttaatcgct gcgggcaaac gcattcatgc cgtttgcccg ttcactgatt
    21061 aattcagcac atacttctca atagcaaatg ccacgccatc ttcaaggtta gatttggtga
    21121 caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg
    21181 catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt
    21241 taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg cgtttatcga
    21301 ggatttcgag gaagtacggc gcacttttca gcacggtgta tttttctttc acttcctgcg
    21361 gaatacgcgc aatggcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga
    21421 actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaatg gtggcaacga
    21481 aagattcatg caccgtgtag tagctgatat ctcggttggc ggtgtacagc gtggtgcggt
    21541 ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cggtagtcgt
    21601 catagctgag agcggtttgc gccacggtgc taccatcagc ggccttctgt accagcgcgc
    21661 cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc aggtaattgt
    21721 gaacacctgc ataagggcga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg
    21781 cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat
    21841 ccatatcgat agcaatgagt ttgatagcca tgagttcccc aggtagattg gttcctgacc
    21901 catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag gggatgcaac
    21961 gcgcattgcg tgctccccat ttgcttagcg gaagagtttc cctttcagca gttccatacc
    22021 tgcggaaagc agatcgttat tggcttgtgg tgacacttca ccttgcggtg agagtgcatc
    22081 aataatcttc ggcaattgtt ctgccagtaa actggaagct gtactggtat ccacgccaag
    22141 tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gattccactt gctcgccgct
    22201 aactgattga ttgctctgtt gattactcag ccaggttgag agaatggccc ctaagccgcc
    22261 actttgcagt ttttccagca gcacctgaat gccgccctgc tcctcaaccc agctcaaaat
    22321 cgcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc
    22381 catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc tgcgggatga
    22441 taattgcgga ttgcaggaat aaaaagggcg gagatgatct ccgcccggac tgacgactaa
    22501 gtcattttat cagcaacaaa aagccattaa atatcgatat tcgccgcttt cagagcgttc
    22561 tcttcgataa acgcacggcg cggttcaacg gcgtcgccca taagcgtagt gaacaactgg
    22621 tcggcagcaa tcgcatcttt aacggtaacg cgcagcatac gacggctttc cgggtccatg
    22681 gtggtttccc acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag
    22741 aggccgcgac gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc
    22801 tgacgacgtt cgccacgttc gataaatgca tcttcttcca gcaagccacg cagtttctca
    22861 cccagcgtgc agatacgacg atattcgccg ccagtgataa attcgtgatc cagcggatag
    22921 tcagtatcca caccgtgggt acgcacgcga acaatcggct cgaacaggtt ttgttcggca
    22981 ttggtatgga catcaaactt ccactggctg ccgtgctgtt ctttatcgtt cagttcgctg
    23041 accagcgcgt tcacccagcg ggtaacggtt tgctcgtcag aaaggtcggt ttccgtcagc
    23101 gtcggctgat agataagctc tttcagcatt gctttcggat aacgacgctc catgcggttg
    23161 atcattttct gcgtcgcgtt gtactcagac accagtttct ctaacgcttc gccagccagc
    23221 gccggtgcac tggcgttggt gtgcagcgtt gcgccatcca gcgcgataga gatctggtac
    23281 tgatccatcg cttcgtcgtc tttaatgtac tgttcctgct tacctttctt cactttgtac
    23341 agcggcggct gagcgatgta gacgtgacca cgttcaacga tttccggcat ctgacgatag
    23401 aagaaggtca acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg
    23461 atgatgctgt gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacaacca
    23521 agcgcggtga taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc
    23581 tcgacgttga ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc
    23641 tgcttcgcag agccgcccgc ggagtcccct tccactaggt atagttcgga aagcgctggg
    23701 tcgcgttcct ggcagtctgc cagtttgccc ggcaggcccg ctaagtcgag cgcaccttta
    23761 cggcgagtca tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc atcgataatt
    23821 ttgccaacca cgattttcgc gtcggttggg ttttccagca ggtattccgc cagcagttcg
    23881 ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc tttggtctgt
    23941 gaggagaatt tcgggtccgg cactttcacg gaaacgaccg caatcaggcc ttcacgcgca
    24001 tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc cttctttgtc catgtaggcg
    24061 ttcagagtac gggtcatcgc cgcacggaaa cctgccaggt gagtaccgcc gtcacgctgc
    24121 ggaatgttgt tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcaacgcc
    24181 acttcgacgc caataccgtc tttttcggtg gaaaagtaga agatattcgg gtggatcggc
    24241 gttttgttct tgttcagata ttcaacgaac gccttgatgc cgccttcata gtggaagtgg
    24301 tcttctttgc cgtcgcgctt atcgcgcaga cgaatggaaa cgccggagtt gaggaacgac
    24361 aactcacgca gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaggtt
    24421 tcaaggcttg gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc
    24481 agcggggcct gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctgg
    24541 ataaccagct ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga
    24601 ccgccggaca ctttatagga gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg
    24661 atcacttccg ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg
    24721 tcatcctgta cagagacaga gttatcggcg tgaatggtga cgataatttc tttacagtga
    24781 cccgcgagcg cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg
    24841 gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct
    24901 ttcaggactt tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt
    24961 attcttgggt taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc
    25021 cgacatgtct attacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc
    25081 ttttaagcgg ctggcaagca gcccgcgacg ctcatcatca agctcagagg caaaatcatc
    25141 tatcaggtag agacaccgcc gcccgctttc acgggtgagg aactctcctt gcgccagacg
    25201 taaggcgcac atcaacagct taagctgtcc acgcgataag gtatcttcca ccggcgcacc
    25261 gtcggcgcga atgcgtaagt ccgctttatg cgggccgtgc gcggtgtagg ttaactggcg
    25321 atcgcgttca aaattacgtt ccagcacctc agcgtactct gtctctttct cccagccgcg
    25381 ctggaaagag aaagtcagag aaaactcagg gagaaattgc ttacaggtat cagccatatc
    25441 ggctgcgata ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat
    25501 cagttcttta tcccacgggc gtagctgttc gtaacgagtc acctggcgca gcgccgcatt
    25561 gcgctgcttg agcaatcgct tgagattgct ccaggcggtg aaaaatccgg gttcgttgtg
    25621 aaagcatccc cagtcgagga atgctcttct gtatttgggg ccgccattga gtaaagtaaa
    25681 cccttctggc gttatcagct gcattggcat caggtgcgcc agctccgcga ctttatgccc
    25741 gtctgtaccg tcaatacgga ctttgctgtc gccttgtttg tctttggtta aaccaatcgc
    25801 cgtctcacgc tcttcgccct gtaatcgtcc gtggagaaca aacgcctcct gctcgtggcg
    25861 aatgacgcga ccaatctgca aactgcgaaa cgcccgaccg tggccgagcg tatagatggc
    25921 ttccagcacg ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga
    25981 taaggcgaga tccgcggttt caatgttgcg gaaatcgcgg atcaacaagc gggtgaggga
    26041 cattacagtc tcattggcat gacaacataa gccgcgctct ggctggccgc atcttcaatc
    26101 tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagtgcgttc
    26161 agaacatcca gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta
    26221 acgtcgagga tctcttccgc ttcttcctgt tctgggttgt tggcggtgat tttcagctgg
    26281 ttttcgctga catacagacg cacgccgcgg aatttctcgt tagagagaat tgccgcacgg
    26341 gcaaacgcct gcttgagcag atcacagcca gcttccagat gcttgtccgg gttcttcggc
    26401 agaacgcgac ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa gataaagtcg
    26461 ccaacgtggg cgcgaatgtt gttgctgcca atctgcacgc gcagcggatt gtcgccgccg
    26521 tcgagcatac gcatcagttc aattacgcct ttacgcggca cgatcaccga atggcttggc
    26581 aaagattgac caattggcat tgaacagact gccagacggt ggccgtcggt cgccacggtg
    26641 cgcagttctt caccttcggt ttcaaacagc ataccgttta agtaatagcg aacgtcctga
    26701 tgtgccatag aaaactgggt cgcttcaatc agacgcttca tcgtcgcctg cggcagggta
    26761 aattcgactt cgctctgcca gtcatcgagg ttcgggaaat ctgccgctgg cagggtagac
    26821 agcgaaaaac ggctacgccc ggagcgcacc agcatccgct caccttccag ctgcacggca
    26881 atttccgcgc cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc
    26941 gtcgcccctg gttcgtgcgg ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga
    27001 tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc
    27061 ggtagcgtag gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc
    27121 tcacgttcta cggtaaattt cataggttta cgatgacaat gttctgatta aattagaaaa
    27181 atcttctttg atatcgtggc tctcttcacg caactgctcg atcttccggc aggcatgaag
    27241 caccgtcgtg tggtcacgac caccaaaggc atcgccaatc tccggcagac tgtggttagt
    27301 cagctctttc gccagcgcca tcgccatctg gcgcggacgg gccaccgagc gggatcgacg
    27361 cttggaaagg agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt
    27421 gtcgatggtg accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa
    27481 gtcgatggtg atcgcccgtc cggtaaagtt ggcgttggca atgacgcggt tcagcgcgcc
    27541 ttccaactca cgtacgttag atcgtagacg cttggcgata aagaacgcca cttcgcccgg
    27601 caaacgaatg tcgttttcgt cggccttttt catcaggatc gccacgcggg tttccagctc
    27661 tggcggttcg atcgccacag tcagtcccca gccgaagcgg gatttcaaac gatcctcaac
    27721 gccgttgatc tctttcggat agcgatcgga ggtgagaatg atctgttgat taccttccag
    27781 cagggcgttg aaggtgtgga aaaactcttc ctgagatcgt tctttattag caaaaaactg
    27841 aatatcgtcg atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc
    27901 gttgttttgc agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac
    27961 tttggcattc ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt
    28021 acccaaaccc gtgccgccat aaaggaacaa cgggttataa gcaccgccag ggttatctgc
    28081 cacctggcga gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa
    28141 cgtgtgtttg acgtttacgt tagaacgata ggtcggttct gccggagccg ggacgttatc
    28201 ccaacccgag cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg
    28261 ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac
    28321 ttcaaaacgc agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt
    28381 attaaggtac ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggccag
    28441 cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg
    28501 taactcatcc tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc
    28561 cactcgaaca aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc
    28621 gaaaagaccc tgcatgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa
    28681 gatcctgcaa aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt
    28741 ttctcacaga ttttcccgat ttatccacag gatgatccag aactcgctaa gtgtaaacga
    28801 tcctgccgca ggccgggcac gatttacgcc gcatattgga aaatttaatg accatagaca
    28861 aaaaatggct taatcgatct aataaagatc caggacgatc cttgcgcttt acccgtcagc
    28921 ccgtataatc ctccacccgg cgcgccatgc tggttttcac tggtgtgagg ccgtacgttt
    28981 tccccgcgaa aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggagtg
    29041 tacaattatt acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt
    29101 tttcggcgga tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg
    29161 aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt
    29221 atggctacta aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt
    29281 ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga
    29341 gttacgcttg ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg
    29401 cacgccgcaa attactattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct
    29461 tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac
    29521 gcgtgaaagc ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc
    29581 gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg
    29641 gcgccgccac tgtcgcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc
    29701 ctgattagtc cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga
    29761 attgaggcat tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta
    29821 ttaaaatgcc accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat
    29881 accagagaac actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg
    29941 tgtctttcat gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac
    30001 agaccacgca gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca
    30061 gtggccaggg gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc
    30121 gtggtggtga tgttgagcag gccctgctgc cggcttaccc gaaagagctg aactctaccc
    30181 agccgttcca gctgctggaa acttcaccgc agtttattta tcaggcacag agcggtctga
    30241 ccggtcgtga tggcccggat aacccggcta acggcccgcg tccgctatat aacgttgaaa
    30301 aagacgctta tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg
    30361 acgcggcagg caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca
    30421 acgtcaacta caacgtgcag aacgctggcg agaaaccgct ggaaatctcc acctttggtc
    30481 agttgaagca atccatcact ctgccaccgc atctcgatac cggaagcagc aacttcgcac
    30541 tgcacacctt ccgtggtgcg gcgtactcca cgcctgacga gaagtacgag aaatacaaat
    30601 tcgataccat tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcaa
    30661 tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct
    30721 acaccgctaa tctgggtaac ggcatcgccg ctatcggcta taaatctcag ccggtactgg
    30781 ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaattcagg
    30841 acaaaatggc agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt
    30901 tcatctctca gccactgttc aaactgctga aatggatcca tagctttgtg ggtaactggg
    30961 gcttctccat tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag
    31021 cgcagtacac ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg
    31081 agcgtctggg cgatgacaaa cagcgcataa gccaggaaat gatggcgctg tacaaagctg
    31141 agaaggttaa cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg
    31201 cgttgtacta catgctgatg ggttccgttg aactacgtca ggcaccgttt gcactgtgga
    31261 tccacgacct gtctgcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga
    31321 tgttcttcat tcagaagatg tcgccgacca ctgtgaccga cccgatgcag cagaagatca
    31381 tgacctttat gccggtcatc ttcactgtgt tcttcctgtg gttcccgtca ggtctggtgc
    31441 tgtactatat cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc
    31501 tggaaaaacg tggcttgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc
    31561 taaaataagg gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccaca
    31621 catccgccac acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg
    31681 ccacgcctcc gggacgtggc ggcgttggca tcctgcgcat ctccggcctc aaagcccgtg
    31741 aagttgccga aaccgtgctg ggtaaactgc ctaagccacg ctacgccgat tatcttccgt
    31801 ttaaagacgc cgatggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga
    31861 actcgttcac cggcgaagat gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg
    31921 acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg gattgcccgc cctggtgagt
    31981 tttccgaacg cgcatttctg aacgataaac tcgacttagc ccaggccgag gcgattgcgg
    32041 atcttatcga cgccagctcg gaacaggcgg cccgttcggc gctaaactcg ctgcaaggcg
    32101 cgttctccgc acgggtcaac catctggtgg aagccctcac ccacttgcgc atttacgtcg
    32161 aagcggcaat tgatttcccg gatgaagaga tcgatttcct ctccgacggc aaaattgaag
    32221 cccagctcaa tgacgtaatt gccgatctcg acgcggtacg cgccgaagct cgtcagggta
    32281 gtttgttgcg cgaagggatg aaagtggtga ttgccggacg tcctaacgcc ggtaaatcga
    32341 gcctgctgaa cgcgctggcg ggccgtgaag cagcaatcgt aaccgatatc gccggaacca
    32401 cgcgtgacgt gctgcgtgag catatccaca ttgacggaat gccgttgcat atcatcgata
    32461 ccgccgggct acgtgaagcc agtgacgaag ttgaacgtat tggtatcgag cgcgcatggc
    32521 aggaaattga acaggcagac cgcgtgctgt ttatggtcga tggcaccacg acagacgccg
    32581 ttgatccggc agagatctgg ccggaattta tcgcccgtct gccagcgaaa ctgccgatca
    32641 ccgtggtgcg caataaagcc gatatcaccg gcgaaacgtt ggggatgagc gaagtgaacg
    32701 gtcacgcgtt aattcgtctt tcggcgcgga ctggcgaagg cgtggatgtg ctgcgtaacc
    32761 atctcaaaca gagcatgggc tttgacacta atatggaagg cggcttcctg gcgcgtcgtc
    32821 gtcacctaca ggcgctggaa caggcggcag agcatttgca gcaaggtaaa gcgcaactgt
    32881 taggtgcctg ggcgggtgaa ctgctggcgg aagagttgcg cctggcgcag cagaacttaa
    32941 gcgagatcac cggggagttt acttcagacg acctactggg gcggattttc tccagcttct
    33001 gtatcggtaa gtaaccgcgc ttgcgaagcc gcattctgac tgtcagatgc ggcttcgctt
    33061 cattgttagc actcctgtta ttcctcaacc cttttttaaa cattaaaatt cttacgtaat
    33121 ttataatctt taaaatagat atttaatatt gcgtcccgaa cgattgtgat t
//
LOCUS       DACADA010000035        33053 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.035, whole genome shotgun sequence.
ACCESSION   DACADA010000035 DACADA010000000
VERSION     DACADA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.035"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            403..1470
                     /locus_tag="ISB17_001663"
     CDS             403..1470
                     /locus_tag="ISB17_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1979606.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1467..4202
                     /gene="rbbA"
                     /locus_tag="ISB17_001664"
     CDS             1467..4202
                     /gene="rbbA"
                     /locus_tag="ISB17_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAP1979607.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4202..5326
                     /locus_tag="ISB17_001665"
     CDS             4202..5326
                     /locus_tag="ISB17_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAP1979608.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5435..6286)
                     /locus_tag="ISB17_001666"
     CDS             complement(5435..6286)
                     /locus_tag="ISB17_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HAP1979609.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(6318..6587)
                     /locus_tag="ISB17_001667"
     CDS             complement(6318..6587)
                     /locus_tag="ISB17_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP1979610.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKCSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(6577..8085)
                     /locus_tag="ISB17_001668"
     CDS             complement(6577..8085)
                     /locus_tag="ISB17_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAP1979611.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKATALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(8078..9436)
                     /locus_tag="ISB17_001669"
     CDS             complement(8078..9436)
                     /locus_tag="ISB17_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP1979612.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSSGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(9513..9794)
                     /locus_tag="ISB17_001670"
     CDS             complement(9513..9794)
                     /locus_tag="ISB17_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP1979613.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(9791..10264)
                     /locus_tag="ISB17_001671"
     CDS             complement(9791..10264)
                     /locus_tag="ISB17_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1979614.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            complement(10289..11035)
                     /locus_tag="ISB17_001672"
     CDS             complement(10289..11035)
                     /locus_tag="ISB17_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1979615.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(11234..11635)
                     /gene="nikR"
                     /locus_tag="ISB17_001673"
     CDS             complement(11234..11635)
                     /gene="nikR"
                     /locus_tag="ISB17_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAP1979616.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(11641..12447)
                     /gene="nikE"
                     /locus_tag="ISB17_001674"
     CDS             complement(11641..12447)
                     /gene="nikE"
                     /locus_tag="ISB17_001674"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAP1979617.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARTSEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(12444..13208)
                     /gene="nikD"
                     /locus_tag="ISB17_001675"
     CDS             complement(12444..13208)
                     /gene="nikD"
                     /locus_tag="ISB17_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAP1979618.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13208..14041)
                     /gene="nikC"
                     /locus_tag="ISB17_001676"
     CDS             complement(13208..14041)
                     /gene="nikC"
                     /locus_tag="ISB17_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAP1979619.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14038..14982)
                     /gene="nikB"
                     /locus_tag="ISB17_001677"
     CDS             complement(14038..14982)
                     /gene="nikB"
                     /locus_tag="ISB17_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAP1979620.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(14982..16556)
                     /gene="nikA"
                     /locus_tag="ISB17_001678"
     CDS             complement(14982..16556)
                     /gene="nikA"
                     /locus_tag="ISB17_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAP1979621.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(16667..17254)
                     /gene="acpT"
                     /locus_tag="ISB17_001679"
     CDS             complement(16667..17254)
                     /gene="acpT"
                     /locus_tag="ISB17_001679"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAP1979622.1"
                     /translation="MYRIVLGKVSTLSAAPLPLGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17309..18358)
                     /locus_tag="ISB17_001680"
     CDS             complement(17309..18358)
                     /locus_tag="ISB17_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP1979623.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            18457..19707
                     /locus_tag="ISB17_001681"
     CDS             18457..19707
                     /locus_tag="ISB17_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979624.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(19711..20268)
                     /gene="dcrB"
                     /locus_tag="ISB17_001682"
     CDS             complement(19711..20268)
                     /gene="dcrB"
                     /locus_tag="ISB17_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAP1979625.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(20341..21006)
                     /locus_tag="ISB17_001683"
     CDS             complement(20341..21006)
                     /locus_tag="ISB17_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAP1979626.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21216..21461
                     /gene="tusA"
                     /locus_tag="ISB17_001684"
     CDS             21216..21461
                     /gene="tusA"
                     /locus_tag="ISB17_001684"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAP1979627.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(21563..23761)
                     /gene="zntA"
                     /locus_tag="ISB17_001685"
     CDS             complement(21563..23761)
                     /gene="zntA"
                     /locus_tag="ISB17_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAP1979628.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAATGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLSNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(23835..24461)
                     /locus_tag="ISB17_001686"
     CDS             complement(23835..24461)
                     /locus_tag="ISB17_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAP1979629.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            24602..24961
                     /locus_tag="ISB17_001687"
     CDS             24602..24961
                     /locus_tag="ISB17_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAP1979630.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(24964..25233)
                     /locus_tag="ISB17_001688"
     CDS             complement(24964..25233)
                     /locus_tag="ISB17_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAP1979631.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(25223..25819)
                     /gene="rsmD"
                     /locus_tag="ISB17_001689"
     CDS             complement(25223..25819)
                     /gene="rsmD"
                     /locus_tag="ISB17_001689"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAP1979632.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            25969..27465
                     /gene="ftsY"
                     /locus_tag="ISB17_001690"
     CDS             25969..27465
                     /gene="ftsY"
                     /locus_tag="ISB17_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP1979633.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            27468..28136
                     /gene="ftsE"
                     /locus_tag="ISB17_001691"
     CDS             27468..28136
                     /gene="ftsE"
                     /locus_tag="ISB17_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP1979634.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            28129..29187
                     /gene="ftsX"
                     /locus_tag="ISB17_001692"
     CDS             28129..29187
                     /gene="ftsX"
                     /locus_tag="ISB17_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAP1979635.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYASHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            29432..30286
                     /gene="rpoH"
                     /locus_tag="ISB17_001693"
     CDS             29432..30286
                     /gene="rpoH"
                     /locus_tag="ISB17_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAP1979636.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            30557..31660
                     /locus_tag="ISB17_001694"
     CDS             30557..31660
                     /locus_tag="ISB17_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1979637.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            31809..32075
                     /locus_tag="ISB17_001695"
     CDS             31809..32075
                     /locus_tag="ISB17_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP1979638.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            32082..32609
                     /locus_tag="ISB17_001696"
     CDS             32082..32609
                     /locus_tag="ISB17_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1979639.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(32606..32989)
                     /gene="panM"
                     /locus_tag="ISB17_001697"
     CDS             complement(32606..32989)
                     /gene="panM"
                     /locus_tag="ISB17_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAP1979640.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
BASE COUNT         7889 a         8607 c         9003 g         7554 t
ORIGIN      
        1 ataataacgc tatgtgatag tcatcaggtt tccctgttac ggaaacctgt gcaacagaat
       61 ctgtgaacct gtacagaata tttttggaaa aaacaccagc gaacggaaga acatcgcgat
      121 gtatcacaag ttgatggcaa tcaaatagac aacatgaata taactgttag tatttaattg
      181 atttcactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
      241 actacccgtt ttcactaagc cttccccatt ggcgctcaat gctctgtgcg ctggtcgtat
      301 ttgccatttc cttcttatcc cggatggaag aatccgctga acgtcgttcg gtggcagttg
      361 taacacgtgt cgattgctta cagggattat ggcgggtcga ttatggataa gagtaagcgc
      421 catctggcgt ggtggggtgt cggggcactg gcggtggctg ccgtcgtggc gtggtggctg
      481 ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat tgaagcgacg
      541 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
      601 cagtttgttc gagaaggtga agtgctggcg aagatggata ctcgcgtgtt gcaggaacag
      661 cgactggaag ccatcgcgca aatcaaagaa gcacaaagtg ccgttgccgc cgcgcaggct
      721 ttgctggagc aacgccaaag cgaaactcgt gccgcacagt cgctggttaa tcaacgccag
      781 gcagaactgg actccgtagc aaaacgtcat acgcgttccc gctcactggc acaacggggg
      841 gcaatttctg cgcaacagtt ggatgacgac cgcgccgccg ctgagagcgc ccgagctgcg
      901 ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc ggcgcgcacc
      961 aatatcattc aggcgcaaac tcgcgtcgaa gcggcacaag ccactgaacg gcgcattgcc
     1021 gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
     1081 gccgagccag gcgaagtgct ggcagcaggc ggtcgggtgc tgaatatggt cgatctcagc
     1141 gacgtctata tgaccttctt tctgccaacc gaacaggcgg gcacgctgaa actgggcggt
     1201 gaagcacgtt tgattcttga tgctgcacca gatctgcgta ttcccgcgac catcagtttt
     1261 gtcgccagcg tcgcccagtt cacgccaaaa actgtcgaaa ccagcgatga gcggctgaaa
     1321 ttgatgttcc gtgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
     1381 gtcaaaaccg gattgccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
     1441 gacgacctgg tggtgaggtt gccgcaatga tgcatctgga actggttccc gtcccgcctg
     1501 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg aacaatatca
     1561 cactcgatat tccggcccgt tgcatggtcg ggctgattgg accggatggt gtcggtaaat
     1621 cgagcttgtt gtcgttgatt tccggtgctc gcgtcattga gcagggcggc gtgatggtgc
     1681 tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc atcgcctgga
     1741 tgccgcaggg gctgggcaaa aatctctacc acaccttgtc ggtgtatgaa aacgtcgatt
     1801 ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt aatgagctgc
     1861 tgaccagcac cggattagca ccgtttcgcg atcgtccggc agggaaactc tccggcggga
     1921 tgaagcaaaa acttgggctg tgctgtgcgt taatccacga cccggaattg ttgattcttg
     1981 atgagccaac aacgggggtt gacccgctct ctcgcgccca gttctgggat ctgatcgaca
     2041 gtattcgcca gcggcagagc aatatgagcg tgctggttgc caccgcctac atggaagagg
     2101 ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca accggtagcg
     2161 ccgaagagtt gcggcagcaa acgcaaagcg ccacgctgga agaagcattt ataaatctgt
     2221 taccgcaagc acaacgccag gcgcatcagg cggtagtgat cccaccgtat caacctgaaa
     2281 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
     2341 ttgatcacgt caatttccgc attccacgcg gggagatttt tggttttctt ggttcgaatg
     2401 gctgcggtaa atccaccacc atgaaaatgc ttaccggatt gctgcccgcc agcgaaggtg
     2461 aggcgtggct gttcgggcag ccggttgatc caaaagatat cgatacccgc cgtcgtgtgg
     2521 gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa aacctggagt
     2581 tacatgctcg tctgtttcat atcccggaag cggaaattcc cgcgcgagtg gctgaaatga
     2641 gcgagcgttt taagctcaac gacgtcgaag atgtattgcc ggagtcattg ccgctcggca
     2701 ttcgccagcg gctatcgctg gcggtggcgg tgattcatcg cccggagatg ctaatcctcg
     2761 atgagccgac ttctggcgtc gatccggtgg cgagggatat gttctggcag ttgatggtgg
     2821 atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg aacgaagcgg
     2881 aacgttgtga ccgtatctca ctgatgcacg ccggaaaagt gctcgccagc ggtacaccgc
     2941 aggagttggt tgagaaacgc ggagccgcca gtctcgaaga ggcatttatc gcttatttgc
     3001 aggaagcggc agggcagagc aacgaagccg aagcgccgcc tgtggtacat gacactaccc
     3061 acgcgccacg tcagggattc agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
     3121 tggaactgcg acgcgatccg gtacgttcga cgctggcgct gatgggaacg gtgatcctga
     3181 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgcttg
     3241 accgcgatca aaccgtcagt agccaggcgt ggacgctcaa tctctccggt tcccgttact
     3301 ttatcgaaca gccaccgctc accagttatg acgagcttga tcgtcggatg cgtgcgggcg
     3361 atatcacggt ggcaattgag atccctccca atttcgggcg cgatatcgcg cgtggtacgc
     3421 ccgtggaact cggtgtctgg atcgacggtg cgatgccgag ccgcgccgaa acggtaaaag
     3481 gctacgtgca ggccatgcac cagagctggc tacaggatgt ggcgagccga cagtccacac
     3541 ccgccagcca aagcgggctg atgaatattg agacgcgcta tcgctataac ccggacgtaa
     3601 aaagcctgcc ggcgattgtt ccggcggtga tcccgcttct gctgatgatg atcccgtcaa
     3661 tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc aacctttacg
     3721 tgacccccac cacccgtagc gaatttttac ttggcaaaca gctgccatac atcgcgctgg
     3781 ggatgctgaa ctttttcctg ctctgcgccc tgtcggtgtt tgtgtttggc gtaccgcata
     3841 aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatc gccaccggaa
     3901 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc ggtacggcaa
     3961 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
     4021 aaggtccagg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
     4081 gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt atcccgttgc
     4141 tgatagccat accgctggtg atgggcttaa gcatcctgct gctgaaaaaa caggagggat
     4201 gatgcgccat ttacgtaata tttttaatct gggtatcaaa gagttgcgca gtctgctcgg
     4261 tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
     4321 gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata tggatcaatc
     4381 gcagttatcg aaccggatcg ttaacagctt ctatcgcccg tggtttttgc caccggagat
     4441 gatcaccgcc gatgagatgg atgccgggct ggacgccgga cgttatacct tcgcgataaa
     4501 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata ttcaggtgaa
     4561 cgttgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
     4621 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
     4681 gctggaaaca cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg gcggggtgat
     4741 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
     4801 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
     4861 catgatggca aaggtctggt cgatggggct ggtggtgctg gtggtgtcgg gattatcgct
     4921 ggtgctgatg gtgaaaggcg tgctgggcgt accgatagaa ggctcgatcc cgctgtttat
     4981 gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta tggggacgat
     5041 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
     5101 gctttccggt ggttccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
     5161 gaccatgccg acgacacact ttgtcagcct cgcgcaggcc atcctctatc gtggtgccgg
     5221 attcgaaatc gtctggccgc agttcctgac gctgatagcg attggcggcg cgttttttac
     5281 cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaatac agtgcccgat
     5341 acgcaagctt atcgggccta cgcgatggtg caatttattg aattctagtg gttttgtagg
     5401 ccggataagg cgtcacgccg catccggcga ggatttattt taccgaccga tgcccctcat
     5461 tcatcatcag gattttgcta tacacatctt cctcgacggc gttttttgct gatgccatca
     5521 ctcgtgccag attcgcctcg ttgtgattat tcacatacgc tttgccgact gcggtgataa
     5581 acgctttgcg cagatcgctg gcgatattca ctttagcgac gcgataatta acgaaactgc
     5641 gtaatatctc cggggcgata ccggagccgc cgtggataac cagcggcacc gggctgactt
     5701 cggcaatgcg cttcagcagg gggatatcga tccgcggtat atcgtccagg ccatgaacgt
     5761 taccaataga aactgccagc atgtcgcaac cggtgcgttc gacaaaggtc ttcacctttt
     5821 ccggttccgt tttgcaatcg gcctcgctga cgtgatcatc ttctttgccg agaatcgccc
     5881 ccagctccgc ttcaaccggc acgccataag atttacagaa atcgacagct tcctgggtga
     5941 aagcgatatt ctcttcaaaa gagaatgcgg caccgtcaat catgaccgag gtaaagcccg
     6001 cgcgcaccgc ctgtttaaca tcctcctggg ttttcccgtg atccagatga agactgaccg
     6061 gaacatccat tttatcggcg tgacgtttca cgatttcata tatgtaatcg tatcccgaca
     6121 gttgtgcatt ggtgggcgca atctgaatga agttgggtaa tcccgaacgc tcaatagcgt
     6181 ttaaaattga aattgtcgtc tccagattgg tggtattaaa cgcgcccgct aatacccgtt
     6241 tttcctgaat gcggtcgagc agtatccgtc cattcactaa tggcatattg aaaacctcat
     6301 tttttaatat cagttaatta ttcagcttca ccgaagcggt cgcgaaataa ggcggtgaga
     6361 gtctgtatgg cctcgttttc atcatcaccg tcgcagcgta tttccaccgt ttttccgcct
     6421 tttattccgg cgcgcataat attcatcata ctggtggcat taatatcatt accctcatag
     6481 tgcagcgtca ctttacagct acattttttg gcggctttcg ccagacaggc cgccggacgt
     6541 gcatgcaggc cagtactgtt tctgacctcc acggttttag tcaacatagt gctcctcccg
     6601 atgatgtaat ggcatcacgc cttcttgctg taatttcaac tgataccagc ttttccattt
     6661 gtgctgataa cgctgtaatt gatgatatct tgcccgattc ggtaacaaac gttgggctat
     6721 ggtaacttgc ggcggcggaa tatccggtgc cgcggttaat aaggctccga cagaaaccgc
     6781 ctcgctaaat gcaattgctt caaccggacg ctgtaaaata tctgccttta attgcagcca
     6841 aagggggtta tgggtcgccg gaccgatcac ttttaacacg ctggtgccag gcacattaaa
     6901 acaggcaaag cagttggcaa attccatggt taatcccagt gagacgctgg aaattaacat
     6961 gtcgatattt aacgtatcgc caagcccata aaataatccg cgggtgtggc gatttttata
     7021 gggtgaaccc gaaccgcgaa gatgcggaat aaagacggga atgtcatcaa gcgaccagtt
     7081 ccccgccagg taatccgcat acccacgatt gagcgacgcg gcgatctcct catcagttag
     7141 ccggaacgtg ttgcgcagcc actccagcgc gaaaccgccc acgggcagag aagcaaacag
     7201 ggtgaactgg ttggcaagcg agtaacagcc gtttgccagc ttgtcacgtt tggcctgttc
     7261 atccagcgtc ggttgtgtat ccagttgcag aatgccttcc gtggtgcccg tcgagttaag
     7321 gatatcgccc ggcatcatct gcaacgcccg cgcacccacc atatggtcat gtccggccag
     7381 cgtcacgctg accggttgcg agaacccaag cgttttgcag agcgactcgc tgacccatcc
     7441 ggcgtgttcg cctggctgca ccaatggcgc aaatgctgaa cagggaacgt gtaacaacgc
     7501 cgtcgctttc gctgaccatt cgcctttgcg cacgtccaga cacagtgtac ggctggctaa
     7561 ggtgatatcc gttcgctgtt cgccagtcag ccgccagagc agcacttccg gtgcatgtag
     7621 ccagcgcagg ccgcgacgat tgcgcagcgg ataatgctcc agcaaccaac gcattttgaa
     7681 ggcagagtaa ttgctgtgca gtggtaatcc gcaaatgtca taaagtgccg cactgtctgc
     7741 ctcgctaagc gttgccagat actcttcacc gcgacggtca taccatgcca gcattggcgt
     7801 caggatctcg ccatgttcgt caagaaacac ccctgactcg ccaaaactgg caatgctgat
     7861 ccgcctgaca ggcagcggtg aagcggcgtt cagttgcgct atcgcctggc ggacctcctg
     7921 ccagagggcg tcgatatcga aatcgacatc gccctgtggg gagatctgtt ttgccgtcac
     7981 gaatttatgc gcgcccaacg tcgttgcgtc caggcaggaa aagcaggtga ctttgcaatt
     8041 ggtggtgcca atatcgataa cgatagcggc gctgttatca ggcataaacc gtgctccgcg
     8101 tcattcggaa aatgccatac agcaccaggg caataaccgg caccagagca atggcgatga
     8161 gattgccgtt gaagatttcc agcagccaga agcggaacgg gttaccgcca tcaaggaaac
     8221 tggaaacctg gccgctggag ccttcggcaa atttgaagtt aacgtctttc gccattccgg
     8281 taataaacgg cgcgattttg gtagcaatcc acaggtcggc aataatcacc gggatcgccg
     8341 ccagaacggt gcggaaaatg ttgccacggc tggctaaagc aatcatcgac gccattaccg
     8401 ccaggttggc aaggtcgcca agcggcagga cttcattacc tgggaggaca aacgcgataa
     8461 agaccgagat tggcgttagc agcagggcgg tggagataat cgccggatta cctacggcaa
     8521 cggcgatatc caggccgata tagagatcgt cacggtcagg gtatttggca ttgagatact
     8581 ttttaatggc ttcagacagc ggtaataaac cttccatcag aatgcgaacc atgcgtggca
     8641 ggatgaacat cacaccgcca atgctgatgc caagcagcaa tacttttttg aaatcgtatc
     8701 cggcaattac gccgagaaaa atgcccagaa tagtgccaac catcatcggt tcgccgaaaa
     8761 tgccaaactt tttctgtacg gtttccggat cgatatgaat acgattgagg ccggggatat
     8821 ggtcgatgat tttgtcgacc aacagaccga ccggaaagaa caccaccgaa gagagcgtcg
     8881 gcaaagagat cccttccagg ccaaagtatt tttgtaccag tggcgcagac cagtcggcga
     8941 gtttaagtac taccaccgca gcaatcgccg ccgccagcaa accaaagaac aggctgccgg
     9001 agctgtaata aaccagcgta ccggcaaggg caaaatgcca gtagttccac atatctacgt
     9061 caacggtgcg ggttttattt aacgccagca tcgccacgtt aatcagaata gtcaggggaa
     9121 taacaaacgg ggcaattggc gaaccccatg taatagcgga aagcggcggc cagcctaaat
     9181 ccaccaccgg taaattaatt ccggtgcgtt caaccatcgc ctgggccgct ggcccgacat
     9241 tgctggtaag aatatcgaaa atggcataaa taccgacaaa accaatgccg accgttaatg
     9301 acgaacgtaa ggctttagcg ggttttactt tgaaaaagag agcaagaata aataacacca
     9361 acggcaacat taccgtggga ccaaatccca atatatattg cacaatatta tagagggtat
     9421 gcgcgatatc gttcataata aacctctttt tcaggcgaga gcgcacctcg cgttagcgcc
     9481 tgatgtagtg tgttgaaatt aattattatt aattactttt ttaggataga aacaatttgc
     9541 gccaatactt tctcttcgcc aataccagta ataattggca agccgttaac caccggcacg
     9601 tcgagttcat aaggcacttt agtcgttgaa acaaccagtg ctacgccgtc ggtatttcca
     9661 ggtaaagagg caacgttggt ttgagaataa ttaacgttaa gaccgtgctc tttacaatat
     9721 tccatgactt tttctgttac tgccgtggat gtggcaatac ccgtggcgca gacaaataaa
     9781 atggtttgac tcatatttca cctcgttctt tcattttgtt taagattgct gtgtgtattt
     9841 ctgttgtttc catcgtaagc agggcgcaca taaagggcgt atcctgaatc atctccataa
     9901 tccaccccag ggcatttaat tgtttgttac tttcgcctaa ggcgagcaaa aaaatcaccc
     9961 tgacaggtac gggatcaggg tcgccgccca tatcggcaaa cactaccggt tccggcagga
    10021 tgccgacggc aattgcattg tgcctgacat gtttatggtc cgtgtgcgga atggcgacac
    10081 acggtggttc ggtaggcagt cccgttgggt acgtcgcctc acgttccagt acatgatcgg
    10141 caaaatcagg gaccaccagt tcggcggtaa ccaacggttg cgctaactga cggatgatct
    10201 gttcaacgct taaccctgcc ggaagtcgtt gaataaaatt ttcctgaaac tgtatgcctt
    10261 gcattttatt cctcttaaaa aagtgtactt aactcacttc ccgccgttgc agcacggtgc
    10321 caagatagta tttatttcct tttaaccaga cgttgccaaa ctcaacgggt aatccgcggg
    10381 agaaaaagac taactgctcc agatgcaaaa cgggtgcatc ttcactgata tctaaaaaat
    10441 gaccgcgttc attaccaatt aatcgcgcgg catagcgact ttccgagtaa cgaatttttc
    10501 tttgcgacaa actttctatt gttggaaata aattgtgttg attaaaatcg atttccacga
    10561 tgccggggca tagctcaata ttgatacggt tctctatcag catcgctttt tcatcaccaa
    10621 ttgaacgtaa acgctcaagg taaagaatat cctgaccggg cgttattctt aatttctctg
    10681 ccacataacg attagccggt tcaatccgtg acgtaataac ttcagtggta aaatgtattt
    10741 tctggctttc cagcgattcc gcgaatgaca ataatccttc acctaacgga tatgccacgt
    10801 tctctttttt gacaaatgtt cccttcccct gaacctgaat caacacgcct tcttttacca
    10861 gttgggcgac cgctttttta acggttcccc gactcacttc cagcatatcc ataatctggt
    10921 attccgaagg aatgcgatcg tcttccacca gatccccggt ataaatactt tctcttatcc
    10981 actccaccac ttgttgatag agcggtattt gtgacgactt actcaatgat ttcatggcgc
    11041 gcacctgaac atttacagtt gtctatggac aatcatggac aacttcaagc gtatagtagg
    11101 tgaacaggcg aaaaagagga tcgaagcgtc tcacatttta ggggaccaaa tcgtgatcaa
    11161 aaaaatgtga cgtgagtcga tatttggcag gagtctactg cgccgtcagg aaataacggc
    11221 gcgttgggag gtgttaatct tccttcggca ggcactgcaa atgcccgtgc cgcacgccgc
    11281 gctgggcgat gacgtcgtcg gcaaaatgtt gtacgtcacc catgtcacct ttcaacacgg
    11341 cgatttccag acagtcgtcg tgattaatgt gcacatgcag agtagcaacc gagagatcgt
    11401 ggtgatgatg ctgggtggag acaatacggc tggctaagtc acgtttttcg tgttcataca
    11461 catacgacag caccgcgaaa ccttgtgtgc cgtgttgttg agtggcttcc tgcgccagag
    11521 cgctacgcag aatatcgcgg atcgcctcgg aacggttgtt ataaccacga cgctggctca
    11581 ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt tgcatttgcg
    11641 ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg ggtaataccg cgttttgtag
    11701 cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accacctggg tttcgacgat
    11761 ttgtccgttg tccatcacca tcacccgctg acagaagcgt tccaccaggc gtaagtcgtg
    11821 ggtgatgaac aggcaggcgg tgccaaactg ttgttgcagc tttttcagca ggcgaatgac
    11881 gcccgcctgt aacacgagat caaggttcga aacggcttca tccagaatca gcagtttcgg
    11941 ttcgaccgcc agcgcgcgtg ccagacagac acgctggagc tggccgccgc ttaactgcgg
    12001 tgggcgtttg tcgagcacgc tgtcgtcgag atcgaccgcc ttcagcattt cgctggtgcg
    12061 cgccagttgt tcggcttttt tcagcgatag caggtggcgc atcggttcac gcaggatctc
    12121 gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc tgaaacacca tttgaatatc
    12181 acggcggaac gctttacgtt gcgcgcgatt gagcttcgcc aacggttcgc cgcgccagct
    12241 aatatttccc tgtgagggcg actctaaccc taccagcagc cgcgcgaggg tactttttcc
    12301 gcagccgctg cgtcccagca gggcgacggt ttcaccgctt ttcagggcca gggaaacgtt
    12361 attcagcacc gcctggtgct gatgttttcc gctaaatcca ccgtgcgcat agtgatggga
    12421 aaggtcggag acgttaagta aagtcatgat gccagctcca taccgtagag ggcgagatgc
    12481 gcggaaacca ggctgcgcgt taccgcatgt ttgggggcgt taaacagcgt ttctacatcg
    12541 ccctgttcga caattttacc gtgagacatt accgccacat cgtctgccag acgcgctact
    12601 acgcccatat catgggtaac cagcagcatt cccggtgctt gtttttgcat aatgctttcc
    12661 agtagatcga ggatgcgtgc ttgtgctacc acgtcgagat cggtggttgg ttcatcggcg
    12721 atgataaacg gtgattcgca cagcaccgcc atcgcaatca tcatgcgctg caacatgccg
    12781 ccgctcatct cgaacgggta gagcttcagc acgcgcgcgg cgttttccag ccccaccgct
    12841 tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ccagcgccag gcaggtttca
    12901 cgcgcgtggg tgtgcatggt gtgcagcgga ttaaaggcac tgcgcgggtt ctgcatgatg
    12961 gtggcaattt tgatgccgcg cagggcgcag ggagaaaccg gttttccatc ggccaaaatt
    13021 tcccccgccg tctggcgaac gcctgcgggc agaatgccca gcgtcgcggc gcaggtcagc
    13081 gacttcccgc tgccactacc gccgactaac gccagcacac gcccacgttg cagggttaac
    13141 gatacgccgt gcaccagcgg ctgcgcggcc tgtagcgcga tattacggag ttcaatctgt
    13201 tgtggcatta gtgtgcgtgc tccgtcacca gatgagggtc cagatgatcg cgcagtgcgt
    13261 cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt
    13321 gcagcggctg ggtccagata tactgacgcg cgtcgttaat catcacgccc cattcggcgg
    13381 tcggcgcggt cacgccgaga ccgaggaagg acatccccgc gacgtgtagc atcatatgac
    13441 cgatatccag cgtcgccagc accagcagcg aggggatcac cgcacctgcc agatgatcaa
    13501 taaacacccg cacatggccc gcgccagaaa gccgtgatgc cagcacaaac tcgcgctggc
    13561 gcagtgaaat caccagactg cgcaccatgc gcgcgtacca tgcccagtgc gaaagggcga
    13621 tagcgataat tacgttggtc aacccggtgc caagcacgcc gaccataaag aacgacagaa
    13681 tcgaggttgg gaaggtcata aacatatccg cgacgcgcat ggtggcctga tcaacgcgcc
    13741 cgccaatcag cccggcgctg ccgccaataa tcagccctaa tgtcagcacc aacagcaggc
    13801 aggccattac cgagccgagc gacacgcggg tcgctgccat cagccgcgag aaaatatcgc
    13861 gccctaaatg atcggtgccc agccagtgct gcgcatccgg cgagagcagg cgcgacggca
    13921 aatcaatagc ctgagggtca tacggcagcc accactggct ggtgagcgca atcagcgcca
    13981 gcagggcgat aatgatcagc gccaggcgaa ccgaccagcg ggaggagagg aaaaagttca
    14041 cgcgtgcgct ccttcatgac gacgaatacg cgggtccagc gcggcgttga gcaaatcgac
    14101 aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact ggatcaccgg
    14161 atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc aggcaaagat
    14221 gttttcgata atcatcgtcc cgccaatcag ttcgccaatg tgcatcccca cggcagtgat
    14281 catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt cattcaggcc
    14341 gcgcagccgc gcccaggtca catgacgctg accggcgacg tccagcatac tggcgcgcag
    14401 caaacgcgcg ttaatcgcca gcgacataaa ggcgatggat accgcaggca aaatgatgtg
    14461 ctgccagccg ccgtaaccca tcgcgggtag ccattgcaga tacaccgaaa acgccatcac
    14521 cagtaaaaac gccagccaga agttaggcat cgacacgccg agaaacgcga tgaaacggac
    14581 ggcgaaatcg ggcaagcgat cgcggtggcg cgccgcccag ataccgagcg gcacggaggt
    14641 gagcagaatt aataccagcg ccgcacccgc cagttccagc gtggcgggca ggaagttcag
    14701 catatcgtcc agcaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc
    14761 cttccatagc caggtgccat actggacgta cagcggctga tccagcccca gcatagtgcg
    14821 ggtagaggcc agcatctccg gcgtcggcgg caggttagat aaacgcaaat aatcaagcgc
    14881 cgggtcgccg gtgccgaggc gcagcatcaa aaaaatgatc accgaggcgg cgagcaccat
    14941 cgggatcagc agcagaaagc ggcgtaatac ataacgcaac attaaggttt caccggttta
    15001 atctgttcga acgggatttc ggtggcgatc ggcgcgtagg ggatgttacc cagttccggt
    15061 tttgaaacca ccatcattga gatgtaactg ataggcagat aaaccgcctc gtcatgcaga
    15121 cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt cgccagcact
    15181 tcgccgatct ctttatcaat cagcggcttg tcggctaaac cttgctgtgc ctggaagtca
    15241 gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag
    15301 gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct
    15361 tcttcgccaa tcagcgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc
    15421 gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga
    15481 ccatttttct cgcggatgtc tttgccagca ggcagcgtcc aaccggcttt ttccagcaac
    15541 gctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgaggttggc gtagggcaca
    15601 gaaggggcaa acagggtgtc ggcgacctgc tgggtaccat acaacgcgtt atcaatcagc
    15661 gattttttgt tcaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct
    15721 ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg agtgtggtaa
    15781 gccgggttct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac
    15841 agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatcacg
    15901 ttaaaggtga tctttttaat cgccggtttt tcgccccagt agttttcgtt acggacgaag
    15961 acatcgtact gattcagttt cgattcctgc aaaacccacg gtccggtgcc aatcggcgct
    16021 tttatcccgt tcatggtttc gtgattttta aattgtgacg gggcaataaa gcggaaagga
    16081 cgcggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt gatttgcagc
    16141 tcgtttttgc tgagtgcttt gacatcaaca atctggtttg ccagctccag ccaggcgtga
    16201 cgttgacggt tatcgagcac tgcacggaag ttttctgccg ccgcctctgc atcaaacggt
    16261 tcgccgttgg agaatttcac gtcatcacgc aaggtgaagg tccaggtttt gccatcttcc
    16321 gaatgggtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
    16381 agcggttcat aaaccatgct ctgggcgaac atttggttag gcgtgtaaag gtgcgggttt
    16441 agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg
    16501 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtaga gaacatggta
    16561 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata
    16621 ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag ttaactgaat
    16681 cgatccattg cacactgtcg gcggtgagcg taaagggggt aggggtgcag atcgccaggc
    16741 ttaaattttc tagctgacaa tggctgactg acagcgagga gttataggtg ctgtctacgc
    16801 tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga
    16861 tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatctcg gcatgttccc
    16921 cgaggctgaa tacggcgttc gccagccagc gccagttggc gcgcgggcga atcacttcga
    16981 tatcgcagcc gacttcgcct tcatcgctca acagcagggc gatatcgtca ccgctatggc
    17041 ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt tcgccataga
    17101 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt
    17161 cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccag tggcagagga gctgcgctta
    17221 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
    17281 gaaagtgcgt atcgtaacac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg
    17341 gccccagtaa aatcgccagt ttgctgccgc ctttggtggt ttccatccag attttacaca
    17401 cgctggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta
    17461 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggtt
    17521 ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc agaatacatt
    17581 cgtaaatacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcac
    17641 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgca
    17701 cccccatcag ctccaacccc agccagacga ttacgcctgt ccataaactg agtagcgtct
    17761 ttaacgccag ataatgcgaa acgcctttaa gtgcgcggtg tagtccggcg atgtgaatct
    17821 gtggattatt caacgcgaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa
    17881 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
    17941 gcgccgtggt gaaggtaacc actttttccg agtccatccg ctgtagtatt cgctccggcg
    18001 acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct
    18061 ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga
    18121 ccagcgcggt gagtgcgatc agcatcacca caaccacaat cgtaatggca acggggcgtt
    18181 gtactccccg acgaataaac caggtgacga gcgggttgag aacgatggca aaaaacagcg
    18241 ccagtaatag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
    18301 gcgaggccaa cttgagcaga atgcgcatgc ccgttttatc gggttgaggg gtttccattg
    18361 gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca atattctgat
    18421 tcttactgct gattttcacg tcagcacatg gtaaaaatga aacactgttg taaaaatgtg
    18481 gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg cctgaatttg
    18541 cgcattgtct ccattgtcat gtttaacttc gccagctacc tcaccatcgg gttgccgctc
    18601 gctgtattac caggctatgt ccatgatgtg atgggcttta gtgccttctg ggcagggttg
    18661 gttatcagcc tgcaatattt cgccaccttg ctgagccgtc ctcatgccgg acgttacgcc
    18721 gatttgctgg gacccaaaaa gattgtcgtc ttcggtttat gcggctgctt tatgagcggt
    18781 ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactg
    18841 tgcctggggc gcgtaatcct tgggattgga caaagttttg ccggaacggg atcgaccctg
    18901 tggggtgttg gcgtggttgg ttcgctacat atcgggcggg tgatttcgtg gaacggtatt
    18961 gtcacttacg gggcgatggc gatgggtgcg ccgttaggtg tcgtgtttta tcactggggc
    19021 ggattgcagg cgttagcgtt aatcattatg ggcgtggcac tggtggccat tttgttggcg
    19081 atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg tgcggtgctt
    19141 gggcgcgtct ggctgtacgg tatggcgctg gcactggctt ccgccggatt tggcgttatc
    19201 gccaccttta tcacgttgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg
    19261 acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt
    19321 atcggcggct taaacgtggc gatgatttgc tttagcgttg agataatcgg cctgctactg
    19381 gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg tcttactggc gggggcgggg
    19441 ttttcgctgg tgttccctgc attgggcgta gtggcggtaa aagcggttcc gcagcaaaat
    19501 cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgaccgga
    19561 ccactggctg ggctggtgat gagttgggcg ggcgttccgg taatttatct ggcggcggcg
    19621 ggactggtcg caatcgcgtt attactgacg tggcgattaa aaaaacggcc cccggtggaa
    19681 atacctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt
    19741 ttctgcggtg gtctgtgctt tttgctgatt gtcagcgggc agcgtaattt gcatggttag
    19801 cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc ctttcgcgga
    19861 gataatgctg tctaactgct gcattttgtg acctttcagc tcaatggctt tattggttac
    19921 cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg ccagcaccgc
    19981 cagatcttct ttcggatcat cgcccatgat gacgatgact gctttctgcc cggtggcgtc
    20041 ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt cggtcatatc
    20101 cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc cggtcgcgtt
    20161 actttctgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac aggccgcaag
    20221 ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg acttcttcct
    20281 ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga taacgaacct
    20341 ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca acagcatatt
    20401 gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt tgaaacagta
    20461 atcgaccagc gcgatttcca tccagtgttc agccataaag gcatccgggc tacgccagaa
    20521 ggcaatgaag aagaaggcca gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg
    20581 tgccagccac cagtgacgac tctgacgcag gcggttaaaa acgtgcacgt cgaggatctg
    20641 ccccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg
    20701 ggcgagcgcg ccgaatcctt gccatgaccc catataaaac agcgatgaga tgacgtagga
    20761 gatcagcaat gcggggatca ttaccgcaaa gatgatgcga cgagccagcg gcgcgccaaa
    20821 aatacgcacg gtcaggtcag tagcgagaaa gataaacgga aagctaaacg cgccccaggt
    20881 ggtatgaaaa ccaaaaatgg agaccggaag ctgaaccaga tagttactgg aggtgatcac
    20941 cagaagatga aataacgata accagaacaa cgccttatag cgttgcgttt gcgagaaaac
    21001 gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac gtgcgacacg
    21061 ctattttata cgtgctgacc cttttttcga gccgccgcat gatactgctt tacgttgaca
    21121 atgcaatggt taattttcac gcaatcgtta acctggtttg cttacggact cacagggcgt
    21181 aaactagcgc cgttttttta tgtgatgaga agaaaatgac cgatctcttt tccagccctg
    21241 accacacact cgacgcactt ggcctgcgct gcccggaacc agtaatgatg gtgcgcaaaa
    21301 cagtgcgcaa tatgcagcca ggtgaaacgt tgctgattat tgctgacgat ccggcgacga
    21361 cccgcgatat tcctgggttt tgtaccttta tggaacacga actggttgct aaagagacgg
    21421 atggactgcc ttatcgttat ttgattcgta aaggcggttg atgggggaag attggcttcg
    21481 atgccgcctt ttcccctcac cctaaccctc tccccagagg agcgagggga ccgatcgcgc
    21541 tcaatgttgc gatcggtttg ccttatttcc tgcgcaacag ccttaacgca ttcgccgtta
    21601 ccagcaccgt cgcaccggta tctgccagca ctgccagcca cagtccggtc atccctaaca
    21661 gcgtcgtgac gaggaaaacg cctttcagcc ccagcgcaat agtgatgttc tggcggatat
    21721 tggcgtgagt ggcgcgtgcc agttcaatca tttgcaccag accgcgcagg tggttatggg
    21781 ttaatgctgc atcggcggtt tccagcgcca cgtctgtgcc gctacccatt gcaatcccga
    21841 tggcggcagc tttcatcgct ggcgcgtcgt taataccatc accgaccatc gccagcggcg
    21901 catgttgatt cagctcggtc accgctttga ctttatcttc cggcaagagg cccgctttaa
    21961 attccagccc cagttccccg gcgattgccg ctgctgcgcg tggattatcg cctgtaagga
    22021 tcaccccttt gacgcccagc gcgttcagtt cactgatagc agttgcagca tcggcgcgca
    22081 gggtatcctg caacgcaatg acacccagca cgtcatcgtt acgtactacc agcactaccg
    22141 tttgcccggc gctttccagt tcattaatca aaccagcaaa tgcatcagca ggatgttttc
    22201 cggcagcgca tatcaacacg cgctcaccgt taacctgcgc ttcaatgcca gacccgacca
    22261 gcgcccgctg tgattgggcg gtaggaatgg cgagttcagc aacctgtgct tcgcgcacaa
    22321 tggcctgtgc cagtggatgc gtcgcgcctt gctcgaccgc cgccgccagt gtcagcagtt
    22381 cagattcact aatacccgtt gccggatgaa tcgcggtaac gcgcggttta ccgacggtca
    22441 gcgtaccggt tttatcaaac gccacctggg taacacgacc cagctgctcc agcgccgcgc
    22501 cgcctttaat caacgcccca cgacgcgctg ctgccgccag cccggaggta atcgccgcgg
    22561 gcgtggagat aactaaagca cacgggcaac caatcagcag cagcgtcagc cctttataaa
    22621 tccactcctg ccagctggcg gcaaacagca gcggcggcac cagcgttacc agcagggcga
    22681 ccgccataat cgctggcgtg taaatacggc tgaaacggtc gataaaccgc tcaataggtg
    22741 cgcggcgctc ttcggcttct tcaatcagtt tcagaatccg gtcaatggcg ctggcacccg
    22801 gttctgacag cacttccagc gttaccagac ggtctacgct ggtggcgcct gcaggaactt
    22861 tatcgcccgt cgcgcgctcc accggaatgg attcgccggt cagggcactt tcatcaaaac
    22921 tggcaaacgg tgagagcagt ttaccgtcgg caggcaaacg cccacctgcc gcaacttcaa
    22981 tcacatcgcc aggccgcagg ctgtttattg ccacctcttc acgctcaccg ttactcagac
    23041 gcgtggcggt ttctggcttc agcgccatta acgcgctgac cccttgacgc gcgcgactgg
    23101 cggcccagcc ttccagtcgt tcaccaatca aaaacagcag caacaccatc gcagcttcag
    23161 ccgttgcgcc aataaacagc gccccagtag cggctacgct cattaaggtt tcaatggcga
    23221 agtagctgcc agatttgatc agccgtaatg cctgacgagc aattgggtac agcccaacca
    23281 gcgtggtcgc gataaacgcc agttgaccaa acggatgatt gaactgctcc agcccccaac
    23341 tgattgccat catcacgata agcgtaatca gcggcagatt ctctttcagg cgtgattctt
    23401 gtggttcgtc ggaggcctgt tcatcgcgca gggaatagcc cgctttttgc accgcagatt
    23461 caacttgtgc ccggatgtca ttgtcggcat ccaccaccag tttttcggtg gcgaacaaca
    23521 cctgtacctg attcacgcct gcaagctggc gcacggcatt ttccactttg cgcgcacagg
    23581 cggcgcagtc cataccgctg actttccagc tatagcgggt gccggagacg ttttcagaga
    23641 gagttggcgt gctggaacat gcgccgtcgc agcaacagtc gttggtgttc tgtaccgtag
    23701 ttagcggttt gaacgcagca aattgagggg ctttcttgcc gtgattgtca ggagtcgaca
    23761 tggcatcctc cggttaagtt ttttctcatt aaccgaagga tacactctgg agtcgactcc
    23821 agagtcaagt tttatcagag atacagcgag cggacgatga ggaagtgacc agcaaagtag
    23881 caggccgcag caatcgcgtt atccgcacgg aagcgtcggc gatagtgact acccagccag
    23941 acaaagttac tgataaacag caacgaagcg ccaacaaacg cagagagcgc cggagcggtc
    24001 ggacggaaga accacagttc acctgccagc cacaccatca ccagcgtcat gccgataaac
    24061 gtgcagatag gccaacggta ctcttccagg cgcgtccaga taatcgccag taacagcgcg
    24121 cccagaacca gcagcaccag tggcagaggc cagaagaaag agagcgtcat ctgactggcg
    24181 aaatagatgg tgtacagcag gtgcgagagg aaaaacgcgc cgatggcgta catcagacgt
    24241 tgacgtggca acagggttag cgcatcgccc agcagtgagg cacacagccc cgccagcacc
    24301 agatagctaa tggcgtcgaa catcggcgct tgccaggcca gcaacagcag gagaagaagg
    24361 gttaacggtt taaacaccca gcgttgccag gttggcccac gatacgatgc atccacagat
    24421 agccatgcgg aaagacagac agcgataaac gaccaaagca tcttagttcc ttgaattcgt
    24481 tattttttcg taagcggaat gcctacggtc ttgcttccag tttagtggcg agggtggacg
    24541 gatgacaaca ccataactgg cagggtatgc ttatttccgc attttccgat gagggataaa
    24601 gatgagtaaa ccacctcttt tctttattgt tatcattggc ttaatcatcg tcgccgcatc
    24661 gtttcgtttt atgcaacaac gacgggaaaa agctgataat gatatggcac cgctccagca
    24721 aaagctggtg gtggtgagca acaagcggga aaaaccgatt aacgatcgcc gttcgcgcca
    24781 gcaggaagtg acgccggcag gcaccagtat gcgctatgag gcaagcttca aaccgcaaag
    24841 cggaggaatg gagcagacgt ttcgcctcga cgcccagcag taccacgccc tgacagtggg
    24901 cgataaaggt acgctgagct ataaaggaac gcgctttgtc agctttgtag gcgaacaata
    24961 aggttatttt ttaactttaa atttcttctg ccagaccagc agttcaaaca cgccgaaaag
    25021 gaaaatccgc accttttcgg cggtggtcat ctgcgggcca tctttcggta aagtggattt
    25081 caatagcgcc agctgcatac catgcatcag cacggtaaaa atcagcgcca cgttaacgaa
    25141 aatattcagc gggcgtggga agggatgcac caggttgagg attaaaaatc cccaaacaca
    25201 gagcattaac aaacgaccaa tattaatcag catcactttc tccttgtgct tcgcgttgat
    25261 acagccgata agccacctga cccgccactt tttcccgatg taatgaccag tttgccggaa
    25321 cagtgggcag acccttttcg acttcgcttt cgacataaat cagggcttcg tcagccagcc
    25381 agccgttatc ttccagtaaa tttatcgtct cttctaacaa gccacggcgg aacggtggat
    25441 cgacaaacac gatattatgc ggtgtgcctt tttgcgccag gaatgacatc gcgttgctgt
    25501 tcaccacgcg tgcattgcct gcttttagtg tcgccagatt cttaattaac tgctgagaaa
    25561 ccgcacgatc catctcaatc aacgtggccc ccgcagcgta gcgcgataac gcttccagcc
    25621 ccagcgcgcc gctcccggcg aagcaatcca gacattgggc gtcaacaatg accggagcca
    25681 gccagttaaa caacgtttcg cgtacgcggt cggtggtggg gcgcagacct ggactatcag
    25741 gaaccgggag tttacggcct cgccactgcc cgccaataat gcggatttgg ccgctgccgg
    25801 aatgattcgg ttttttcatg ataaattgct caatccgcca gataacacat acttgcaggc
    25861 ggtgatgtga attaagttaa gtgatagact atttcatcat ttttttagct gctatgtaca
    25921 tagcgttaac gctgtgccat gaagcaacag cgaggagtgt agtcgcaaat ggcgaaagaa
    25981 aaaaaacgtg gctttttttc ctggctgggc tttggtcaaa aagagcagac cccggaaaaa
    26041 gagacagaag ttcagaatga acaaccagtt gtagaagaaa tcgttcagga gcaagagcct
    26101 gtgaaggctt ctgaacaagc cgttgaagag cagccgcagg cgcatactga agccgaggcg
    26161 gaaacttttg ctgccgacgt tgtggaagtt actgaacagg ttgctgaaag tgaaaaagcg
    26221 cagcctgaag cggaagtcgt tgcacagccg gaaccggtcg tagaagaaac gccggagcca
    26281 gtggctatcg aacgtgaaga actgccgttg ccggaagacg tcaacgccga agcggtttcg
    26341 ccagaagagt ggcaggccga agcggaaacc gtagagattg tcgaagcggc ggaagaagaa
    26401 gcggctaaag aagaaatcac cgacgaagag ccggaggcac aggcgctggc tgccgaagtg
    26461 gcagaagagg cggtaatggt ggtttctccg gcagaagaag atcagccggt ggaagaaatc
    26521 gctcaggagc aggaaaaacc gaccaaagaa ggtttcttcg cgcgcctgaa acgcagcctg
    26581 ttaaaaacca aagaaaatct cggttccgga tttatcagcc tgttccgcgg taaaaaaatc
    26641 gacgatgatc tgtttgaaga gctggaagaa cagctgttga tcgccgatgt gggcgtggaa
    26701 accactcgta aaattatcac caatctgaca gaaggcgcat cccgcaagca gcttcgtgat
    26761 gccgaggcgc tctatggcct gctgaaagaa gagatgggcg agattctggc gaaagtcgat
    26821 gagccgctga atgttgaagg taaaacgccg ttcgtgatcc tgatggtggg cgtcaacggt
    26881 gtgggtaaaa ccacgacgat tggtaagctg gcgcgtcagt ttgagcagca gggtaaatcg
    26941 gtgatgctgg cggcaggcga tactttccgt gcggcagcgg ttgaacagct tcaggtctgg
    27001 ggtctgcgca acaatattcc ggtgattgcc cagcataccg gtgcggattc cgcttctgtt
    27061 atcttcgatg ccattcaggc cgctaaagcg cgtaacatcg acgtcctgat tgccgatacc
    27121 gccggacgcc tgcaaaacaa atcgcacctg atggaagagt tgaagaaaat cgtccgcgtg
    27181 atgaagaaac tcgacgttga agctccgcat gaagttatgc tgaccatcga tgccagcacc
    27241 gggcagaatg cggtaagcca ggccaaactg ttccacgaag ccgttggctt aaccggcatc
    27301 acgctaacga aactggacgg cacggcgaaa ggcggggtaa ttttctcggt ggctgaccag
    27361 tttggtatcc ctatccgcta cattggtgtc ggcgaacgta ttgaggattt gcgtccgttt
    27421 aaggcggacg actttataga ggcacttttt gcccgagagg attaacaatg attcgctttg
    27481 aacatgtcag caaggcttat ctcggtggga gacaggcgct gcagggcgtt acgttccata
    27541 tgcagccggg tgagatggcg tttctgaccg gtcattccgg cgcagggaaa agtaccctcc
    27601 tgaagctgat ctgtgggatt gagcggccca gcgccgggaa aatctggttt agcggccatg
    27661 acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg ccgccagatt ggcatgattt
    27721 tccaggatca ccatctactg atggaccgta ctgtctatga taacgtggcg atcccgctga
    27781 ttatcgccgg tgccagcggt gatgacattc gtcgccgggt gtcggcggcg ctggataaag
    27841 tcgggctact ggacaaagcg aagaacttcc ctattcagct ttccggcggt gaacaacagc
    27901 gtgttggcat tgcccgcgcg gtggtgaaca agcccgcggt actgctggcg gacgaaccga
    27961 ctggtaacct ggacgacgcg ctgtcggaag gcattttacg tctgtttgaa gagtttaacc
    28021 gcgttggagt aaccgtattg atggcaacgc acgacatcaa cctgatttcg cgacgttcct
    28081 atcgcatgct caccctgagc gatggtcact tgcatggagg cgtgggccat gaataagcgc
    28141 gatgcaatca atcacattcg gcagtttggc gggcgtctgg atcgcttccg taaatcggtc
    28201 ggcggctctg gcgacggcgg tcgtaacgcg ccgaaacgcg cgaaatcctc gccaaaaccg
    28261 gtaaatcgca aaaccaacgt tttcaacgaa caggtacgct atgcctccca cggcgcattg
    28321 caggatctga aaagcaaacc gttcgccacg tttttaacgg tgatggttat cgccatctcc
    28381 ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg ttaaccaggc ggcgacgcaa
    28441 tattatccgt caccgcaaat cactgtttat ctgcaaaaaa cgctggatga tgacgccgct
    28501 gcgggcgtgg tggcacagtt gcaggccgag caaggcgtgg agaaagtgaa ttatctttct
    28561 cgtgaagacg cactgggcga gttccgtaac tggtctggtt ttggtggtgc gctggatatg
    28621 ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc cgaaactcga ttttcagggg
    28681 acggagtcac tgaatacgct gcgtgatcgt atcacgcaga ttaacggcat tgacgaagtg
    28741 cggatggatg acagctggtt tgcccgcctg gcggcgttga ccgggctggt cgggcgcgtt
    28801 tcggcaatga tcggcgtatt gatggtggcg gcagtattcc tcgtcatcgg taacagtgtg
    28861 cgcctgagta tctttgctcg ccgtgattct attaacgtgc agaaactgat tggtgcgacg
    28921 gatggtttca tcctgcgtcc gttcctgtac ggtggcgcat tgctgggatt ttctggcgca
    28981 ttgttgtcac taattttgtc agaaattctg gtgctgcgtt tgtcatcggc ggttgcggaa
    29041 gtggcacagg ttttcggaac gaagtttgat atcaatggct tatcgttcga tgaatgcctg
    29101 ctattgctgc tggtatgctc gatgattggc tgggtggcag cgtggcttgc cacggtacaa
    29161 catttacgcc actttacgcc tgaataataa aagcgtgtta tactctttcc ctgcaatgga
    29221 ttccgtagca gggaaagaga ccccgttgtc tcttcccggt attttatctc tatgtcacat
    29281 tttgtgcgta atttattcac aaacttgcat tgaacttgtg gataaaatca cggtctgata
    29341 aaacagtgaa tgataacctc gttgctctta agctctggca cagttgttgc taccactgaa
    29401 gcgccagaag atatcgattg agaggatttg aatgactgac aaaatgcaaa gtttagcttt
    29461 agccccagtt ggcaacctgg attcctacat ccgggcagct aacgcgtggc cgatgttgtc
    29521 ggctgacgag gagcgggcgc tggctgaaaa gctgcattac catggcgatc tggaagcagc
    29581 taaaacgctg atcctgtctc acctgcggtt tgttgttcat attgctcgta attatgcggg
    29641 ctatggcctg ccacaggcgg atttgattca ggaaggtaac atcggcctga tgaaagcagt
    29701 gcgccgtttt aacccggaag tgggtgtgcg cctggtctcc ttcgccgttc actggatcaa
    29761 agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc gtcaaagttg cgaccaccaa
    29821 agcgcaacgc aaactgttct tcaacctgcg taaaaccaag cagcgtctgg gctggtttaa
    29881 ccaggatgaa gtcgagatgg tggcccgtga actgggcgta accagcaaag acgtacgtga
    29941 gatggaatca cgtatggcgg cacaggacat gacctttgac ctgtcttccg acgacgattc
    30001 cgacagccag ccgatggctc cggtgctcta tctgcaggat aaatcatcta actttgccga
    30061 cggcatcgaa gatgataact gggaagagca ggcggcaaac cgtctgaccg acgcgatgca
    30121 aggtctggac gagcgtagcc aggatatcat ccgcgcgcgc tggctggacg aagacaacaa
    30181 gtccacgttg caggaactgg ctgaccgtta cggtgtttcc gctgagcgtg tgcgtcagct
    30241 ggaaaagaac gcgatgaaaa aattgcgcgc tgctattgaa gcgtaatttc cgctattaag
    30301 cagagaaccc tggatgagag tccggggttt ttgttttttg ggcctctaca ataatcaatt
    30361 ccccctccgg caaaacgcca atccccacgc agattgttaa taaactgtca aaatagctat
    30421 tccaatatca taaaaatggg gtatgtttta gcagagtatg ctgctaaagc acgggtagct
    30481 acgtataaaa cgaaataaag tgctgcacaa caacatcaca acacacgtaa taaccagaag
    30541 agtggggatt ctcaggatga acataaaggg taaagcgtta ctggcaggat gtatcgcgct
    30601 ggcattcagc aatatggctc tggcagaaga tattaaagtc gccgtcgtag gcgcaatgtc
    30661 cggtccggtg gcgcagtacg gtgaccagga gtttactggc gcagagcagg cggttgcgga
    30721 tatcaatgct aaagggggta tcaaaggcaa caaactgcaa atcgtaaaat atgacgatgc
    30781 ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg gcattaaata
    30841 cgtgattggt cacctctgct cttcatcaac gcagccagcg tctgacatct acgaagacga
    30901 aggcatttta atgatcaccc cagcggcaac cgcaccagag ctgaccgccc gtggctatca
    30961 gctgatcctg cgcaccaccg gcctggactc cgaccagggg ccgacggcgg cgaaatatat
    31021 tcttgagaaa gtgaaaccgc aacgcattgc tatcgttcac gacaaacagc agtacggcga
    31081 aggtctggcg cgtgcagtgc aggacggcct gaagaaaggc aatgctaacg tggtgttctt
    31141 tgatggcatc accgccgggg aaaaagattt ctctaccctg gtggcgcgtt tgaagaaaga
    31201 gaatatcgat ttcgtttact acggcggtta tcacccggaa atggggcaaa tcctgcgtca
    31261 ggcacgcgcg gcagggctga aaactcagtt tatggggcca gaaggcgtgg ctaacgtctc
    31321 gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc cgaagaacta
    31381 cgatcaggtt ccggcgaaca aaccgattgt tgacgcgatc aaagcgaaaa aacaggaccc
    31441 aagcggcgcg ttcgtctgga ccacctacgc cgcgctgcaa tctttgcaag cggggctgaa
    31501 tcagtctgac gatccggcgg agatcgctaa atacctgaaa gcgaactcgg ttgatacggt
    31561 gatggggccg cttagctggg atgagaaagg cgatctgaaa ggctttgagt tcggtgtgtt
    31621 tgactggcac gccaacggca cggcgactga tgcgaagtaa cagatagata aatgaaaata
    31681 cccgatgcgc tgaactgcca tcgggttttt ttatatttat cgaaatggtt gtagaaagat
    31741 tgaacaattc aggatatgct tatgtacggt gatatgccgt acatattata cggagcagga
    31801 gagttcacat gtctgctgtt aaaaagcagc gtattgatct gcgtttaact gacgatgaca
    31861 aaagtatgat tgaggaagcg gcagcgatat ctaatcagtc cgtcagccaa tttatgttga
    31921 acagcgcttc gcagcgagct gcggaagtga ttgaacagca tcggcggatg attctcactg
    31981 aggaatcctg gacgcgggtg atggatgcgc tgagtaatcc accgtcacca ggtgaaaagc
    32041 taaaacgtgc ggcaaaacgt cttcagggaa tgtaatactt tatggatgat ctgacgatag
    32101 agattctgac cgatgatgca gattatgatc tacagcgatt cgactgcggc gaggaagcat
    32161 taaatctctt tctgacgaca catctggttc gtcaacatcg caataaaatt ctgcgagcgt
    32221 atatcctgtg tcgcaacact ccagaacgtc aggtgctggg atattacaca ttatccggca
    32281 gttgttttga acgagccgca ctgccctcga aatcgaaaca gaaaaaaatt ccctacaaaa
    32341 atattcccag cgtaactctt gggcgtctgg caattgatcg ttcattacag gggcagggat
    32401 ggggagcaac actggttgct catgccatga aagttgtctg gtcagcctct ttagcggtag
    32461 gtattcacgg tctttttgtc gaggcgctga atgaaaaagc ccatacgttt tatcagtcgc
    32521 tgggctttat cccattggtc ggagaaaacg aaaatgcgtt atttttcccg accaaatcta
    32581 tagagctact ttttacacca gacgattaac gcttctccca gccaccctgt tgtgccgtaa
    32641 atcccagtgc tcgcataaac gccgtcatca cgccgcgatc ttccacgcca gcatccgcca
    32701 tccaccaaca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga tattgcccca
    32761 caccgcgacg tcgggttact tcccgcacgc gtagagaatc cagtgccccc tcggtgccgc
    32821 ttaaggttac ccgcacggca gcgagcaggc gctcgttaaa acgcgcggcg tagatgcggt
    32881 ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt tgcaggtcaa
    32941 tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg gggaacccgt
    33001 gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct ttc
//
LOCUS       DACADA010000036        32883 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.036, whole genome shotgun sequence.
ACCESSION   DACADA010000036 DACADA010000000
VERSION     DACADA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.036"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(30..1073)
                     /locus_tag="ISB17_001698"
     CDS             complement(30..1073)
                     /locus_tag="ISB17_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1979641.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            1423..1524
                     /locus_tag="ISB17_001699"
     CDS             1423..1524
                     /locus_tag="ISB17_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979642.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHLASDLCGGEA"
     gene            1521..1976
                     /gene="ybcN"
                     /locus_tag="ISB17_001700"
     CDS             1521..1976
                     /gene="ybcN"
                     /locus_tag="ISB17_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HAP1979643.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            1976..2146
                     /locus_tag="ISB17_001701"
     CDS             1976..2146
                     /locus_tag="ISB17_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979644.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            2139..2429
                     /locus_tag="ISB17_001702"
     CDS             2139..2429
                     /locus_tag="ISB17_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAP1979645.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            2426..2788
                     /locus_tag="ISB17_001703"
     CDS             2426..2788
                     /locus_tag="ISB17_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP1979646.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            2785..2925
                     /locus_tag="ISB17_001704"
     CDS             2785..2925
                     /locus_tag="ISB17_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HAP1979647.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPSSVLTDYRILKNYAKTLKGA
                     AA"
     gene            2922..3611
                     /locus_tag="ISB17_001705"
     CDS             2922..3611
                     /locus_tag="ISB17_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1979648.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQLYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     IFSQQDIAKLD"
     gene            3933..4238
                     /locus_tag="ISB17_001706"
     CDS             3933..4238
                     /locus_tag="ISB17_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1979649.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            4225..4701
                     /locus_tag="ISB17_001707"
     CDS             4225..4701
                     /locus_tag="ISB17_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAP1979650.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            4918..5100
                     /gene="rzoD"
                     /locus_tag="ISB17_001708"
     CDS             4918..5100
                     /gene="rzoD"
                     /locus_tag="ISB17_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HAP1979651.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(5191..5484)
                     /gene="iss"
                     /locus_tag="ISB17_001709"
     CDS             complement(5191..5484)
                     /gene="iss"
                     /locus_tag="ISB17_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HAP1979652.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            5965..6291
                     /locus_tag="ISB17_001710"
     CDS             5965..6291
                     /locus_tag="ISB17_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAP1979653.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            6498..6680
                     /locus_tag="ISB17_001711"
     CDS             6498..6680
                     /locus_tag="ISB17_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979654.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            7244..7789
                     /locus_tag="ISB17_001712"
     CDS             7244..7789
                     /locus_tag="ISB17_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP1979655.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     DVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            7764..9689
                     /locus_tag="ISB17_001713"
     CDS             7764..9689
                     /locus_tag="ISB17_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAP1979656.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHCMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLHVDEAINKTYT
                     RRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            9686..9892
                     /locus_tag="ISB17_001714"
     CDS             9686..9892
                     /locus_tag="ISB17_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lambda prophage-derived head-to-tail joining
                     protein W"
                     /protein_id="HAP1979657.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            9889..11490
                     /locus_tag="ISB17_001715"
     CDS             9889..11490
                     /locus_tag="ISB17_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1979658.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            11471..12790
                     /locus_tag="ISB17_001716"
     CDS             11471..12790
                     /locus_tag="ISB17_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAP1979659.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNLASDAVNDLLNTPV"
     gene            12800..13132
                     /locus_tag="ISB17_001717"
     CDS             12800..13132
                     /locus_tag="ISB17_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAP1979660.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            13188..14213
                     /locus_tag="ISB17_001718"
     CDS             13188..14213
                     /locus_tag="ISB17_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAP1979661.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLV
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQLEGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            14255..14653
                     /locus_tag="ISB17_001719"
     CDS             14255..14653
                     /locus_tag="ISB17_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAP1979662.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVTLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            14665..15018
                     /locus_tag="ISB17_001720"
     CDS             14665..15018
                     /locus_tag="ISB17_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979663.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            15030..15608
                     /locus_tag="ISB17_001721"
     CDS             15030..15608
                     /locus_tag="ISB17_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979664.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGDSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            15605..16000
                     /locus_tag="ISB17_001722"
     CDS             15605..16000
                     /locus_tag="ISB17_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979665.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            16008..16748
                     /locus_tag="ISB17_001723"
     CDS             16008..16748
                     /locus_tag="ISB17_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979666.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELSAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            16764..17186
                     /locus_tag="ISB17_001724"
     CDS             16764..17186
                     /locus_tag="ISB17_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAP1979667.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKRQAEPAGSDSN
                     RQVTVEDVIRTGAFLVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            17168..17602
                     /locus_tag="ISB17_001725"
     CDS             17168..17602
                     /locus_tag="ISB17_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAP1979668.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            17595..20156
                     /locus_tag="ISB17_001726"
     CDS             17595..20156
                     /locus_tag="ISB17_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1979669.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYAARQEELNKALKDGKILQADYNTLMAAAKKDYETT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSVQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            20153..20482
                     /locus_tag="ISB17_001727"
     CDS             20153..20482
                     /locus_tag="ISB17_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979670.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWQQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            20482..21180
                     /locus_tag="ISB17_001728"
     CDS             20482..21180
                     /locus_tag="ISB17_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP1979671.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            21185..21928
                     /locus_tag="ISB17_001729"
     CDS             21185..21928
                     /locus_tag="ISB17_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979672.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPKGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            21865..22467
                     /locus_tag="ISB17_001730"
     CDS             21865..22467
                     /locus_tag="ISB17_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP1979673.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            22528..26010
                     /locus_tag="ISB17_001731"
     CDS             22528..26010
                     /locus_tag="ISB17_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP1979674.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLLNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKETIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVEASARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PTFSLTPDGRLSARNADISGNVNANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVG
                     KAFPRNGSYASGTITVTVYDDQAFDRQIVVPPVLFRGGKHENFNSNNQQSYWYSTCKL
                     QVLKNGQEIFQQPATDVSRVFSSVIDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLL
                     VVVMKKSTAGISIS"
     gene            26069..28090
                     /locus_tag="ISB17_001732"
     CDS             26069..28090
                     /locus_tag="ISB17_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979675.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVALLVEGFPPSHAGTITVYEDSKPGTLNDFLGAATEDDVCPEA
                     LYRFEKMVEEAARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPCANIVPPLPDVWIPFNDSLDMITGFSP
                     SYKKIVIGDDEITMPGDKIVKFKRASTATYINKSGQLKLAEVDEPRFERDGLLIEGQR
                     TNYLRNSNKPDSWTVHSALNKTFGTDKQGFNYATVTPTESIVGTTGGYTVHGVVAADR
                     FPLASGECFTFSCRVKGAKARCRLRVSVIIGGTDTFSADSYLDLDTRIATVSGNTSLI
                     TAKAEQQGEWTYYEATYTANTDIDTVNCAFYMTNKISNETFYDDSTLTMTTPQIELGN
                     TASSFIVTTMPTTRASDVVTIPSQNNLSTRPFTVLCEVSRNWSTPPNVAPRIFDVGGH
                     SIDDNYLSLGFVATGKISANVGMVQPQIASDGERFIVGVRAKSDLSVNAICNGNYTTN
                     LNGKIFGVTATSYRFGGQTAAGTRHLFGHIRNFRVWFKELNDRQIKEAV"
     gene            28087..28365
                     /locus_tag="ISB17_001733"
     CDS             28087..28365
                     /locus_tag="ISB17_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979676.1"
                     /translation="MKDLTLKFPGNREFKSFLSSLDWEEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCVRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            28378..28671
                     /locus_tag="ISB17_001734"
     CDS             28378..28671
                     /locus_tag="ISB17_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979677.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(28763..29620)
                     /gene="sitD"
                     /locus_tag="ISB17_001735"
     CDS             complement(28763..29620)
                     /gene="sitD"
                     /locus_tag="ISB17_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAP1979678.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(29617..30474)
                     /gene="sitC"
                     /locus_tag="ISB17_001736"
     CDS             complement(29617..30474)
                     /gene="sitC"
                     /locus_tag="ISB17_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAP1979679.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(30471..31298)
                     /gene="sitB"
                     /locus_tag="ISB17_001737"
     CDS             complement(30471..31298)
                     /gene="sitB"
                     /locus_tag="ISB17_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAP1979680.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEDVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(31298..32188)
                     /gene="sitA"
                     /locus_tag="ISB17_001738"
     CDS             complement(31298..32188)
                     /gene="sitA"
                     /locus_tag="ISB17_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP1979681.1"
                     /translation="MLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         8082 a         7959 c         9448 g         7394 t
ORIGIN      
        1 gaatggggag ggcttagccc tccctctttt tacaggttag cgtttgggtt ttgttgttgg
       61 ggagctgttt ttataggcgc tcccaggttt ttagtaaacc actcaacgac tctgtttata
      121 ataattggct gataccatgt cttatcgcca tgctcggccc cttctaccag cacgtactca
      181 gcgttatcac cgttcttctt caacatcctg aatagtttgg cgctttgctc aggtgaaacc
      241 agagtgtctt tgctaccatg cataataaga aatgggggtt ttactccttt catatgtccg
      301 attggactgg cgtttagcgc tttttctttt gacgctgtga tgggggctcc cgcaaaattt
      361 ctgaatgcag ggccattgat cattaaggct tcggttacgg caggagagcg atgaacctcc
      421 tgcactgatt cagggaaccc ctcgccaata ttcaacaagt cagaaatccc ataaagtgtg
      481 gcaactgcct gaacatctga ggattgctga agaaagtcac ctttatcaaa ggttttgtca
      541 ccatttgtag ttcccatcat ctgggcaagc catccaccgg cagagtcacc cagaactccg
      601 attctttgag gatcaatccc ataattgctg gcatgctctc tcaggtaacg tattgcagct
      661 tttccatcct caactggtgc tggaaatgta tcaggaattg ttctgtattc tacagcggcc
      721 acaacaaaac cagcttctgc cagagccatt ctcatttcaa taaatttgtc atgttcagaa
      781 gacatgaatc cgccgccggg ataataaata atggctggtt ttaaatcatt tgttcgcggg
      841 acaagaactg acatgtgaag ctgtctgaca gaacgagttc cttttatctg ggaataaaca
      901 acatcaccaa tgagatcgac ctggtttctg gtcggtgaaa cactaatgat atctgcaccc
      961 ggggtgtaac caggaagatt agtctggaca ggtgttgcac aactcccgac cgacatggcc
     1021 attgacatac catacaatag tgttgcagat gataatatct tgttaatttt catgttttac
     1081 atgccttgac ttatcggatg aaattctctt actaaattta acggcagttt aatagccgta
     1141 caactcgtgg tagccaggga aatatcccag tcgtggttaa ggttgtactt gacggctatt
     1201 atttcaacaa tgcttaaagt gagagcttaa ggttatgcgt atgttttaca gtccgagtag
     1261 attctgacca atagcttctt tgtataattc tgtcagaaat aaatcttggc ttgcatgaag
     1321 tttgagacct tatcttgttt gattatgaat aatcaattcg ccataattgt atcaccggag
     1381 cctgaacaac tccggtgact tctgcgctaa acggggacgt ttatgcgcac acacaatcca
     1441 aactctcttc tcccttcaca gatgcagaaa tgcacctgcg tttttttgca tctagcgtct
     1501 gacctctgtg gaggtgaagc gtgaacctac cacaagatgg catcaaactg catcgcggta
     1561 acttcatcgc tatcggccag cagatccagc cttatctgga gaacggaaaa tgctttcgca
     1621 tggtgcttaa accgtggcgt gagaaacgca gtctttccca gaatgcactc agccacatgt
     1681 ggtacagcga aatcagtgaa tacctcatca gcagggggaa atcgttcgct accgcagcat
     1741 gggtaaaaga tgctctcaaa cacacatacc tcggttatga aaccaaggac ctggttgatg
     1801 tcgtaaccgg cgaaatcact actatccagt cgttacgcca tacctccgat ctcgataccg
     1861 gagagatgta tgtcttcctg tgtaaggttg aagcctgggc ggtgaatatt ggctgccacc
     1921 tgactattcc gcagagctgc gagttccagc tgctgcgcga caagcaggag gcgtaatggc
     1981 tacaccgctt attcgtgtca tgaacggaca catctacaga gtaccaaatc gtcgtaagcg
     2041 taagcctgag ctgaaaccat ccgaaatacc aacactgctc ggatataccg ccagcctggt
     2101 tgataaaaaa tggttgcgac tggcagcaag gaggaatcat ggctgacttg agaaaagcag
     2161 cgcgtggtcg ggaatgccag gtaagaatcc ctggcgtatg taatggcaat cctgaaacgt
     2221 cagtactggc acatatccgg ctggctggat tgtgcggtac cggtatcaaa ccgccagacc
     2281 tgattgccac cattgcatgt tctgcctgcc acgacgaaat cgaccgtcgc acgcattttg
     2341 ttgacgctgg atatgcaaaa gaatgtgcgc tggaaggtat ggcgagaaca caggttatct
     2401 ggctgaaaga gggggtaatt aaggcgtgaa tacttactgc atcacactac cctggccgcc
     2461 gagcaataat cgctattacc gccataatcg cggacgcacg catatcagcg cagaggggca
     2521 ggcataccgc gataatgtcg cccgaatcat taaaaacgca atgctggata tcggcctggc
     2581 tattcctgtg aaaatcagca ttgagtgcca catgccggat cgccgtcgcc gtgaccttga
     2641 taatctgcaa aaggccgctt ttgacgcact caccaaagca ggtttctggc tggatgatgc
     2701 tcaggtcgtt gattaccgcg ttgtgaagat gcccgttacc aaaggtggga agctggagct
     2761 gactatcact gaactgggag atgaatgatg tttgagtctt atatggcaga acgtcttcgc
     2821 cgccgctggg tgcgcctgcg cttatatcgt tttcctagtt ctgttttgac cgattaccgg
     2881 atactgaaga attacgccaa aacactgaaa ggagctgccg catgaatacc caatatttac
     2941 agtatgtccg cgagcaactc attgtggcta ccgctgattt gagcggagca acgaaaggac
     3001 agcttgaagc ctggctggag catgcacaat ttgatactgg tacatacaaa cgaaagaagc
     3061 cgcgcattct ggatgaggta actggcagga tgattacgct ggataatccg ccgatttccg
     3121 gtaagcagtt gtacgcaaaa ggttcatcca ttgcactggt cagccaggtt gagttctcaa
     3181 cctcgtcatg gcgccgcgcg gttctgtctc tcgaagaaca tcagaaagcg tggttgctgt
     3241 ggagttacag cgaaagtgtt cgctgggaac atcaggtcac cataacgcag tgggcatgga
     3301 gcgagtttaa gactctgttg ggtaccagga aaattgcagg taaaacactg gaacgtttga
     3361 agaagttgat ctggctggcg gcacaggatg tgaagaacga gctggcaggg cgtaagacct
     3421 atgaatacca ggagctggca tcactggtgg gagtgacatc aaaaaactgg tctgagacat
     3481 ttactgaacg ctgggttgca atgaagcaca tttttctaca gcttgatagc caagctttat
     3541 tgcttttaac gaaaacacgt tcaaaacaaa agaccatatt ttcacagcaa gatattgcaa
     3601 aactggatta aaaatcatat attttatgta aatctgatat tttgccaatg ttgtacgcac
     3661 tggcagtaat ccaaattcaa gcccgaggtt taaagccttg ggcttttctg tttctgaacg
     3721 gtgagtagcc ttccaaccta ccccagccag ggtgtcttca gctgttgagt tgatattgct
     3781 taaccctctg ttgccagcta catgctggct tttttattcc aggcttgcgg ggagcatcaa
     3841 ctccgtgctt tgtcgttaaa ttaccccgtg agcctgattt ctgacattta acgtcccggc
     3901 cttttgtcgg cggcgaaaca ttggctattc atatgcacga aaaagagagc cttgccggag
     3961 cgttctggct cgttttgctg atcatcgcag gttggggcgg tctggtccgc tacctgatag
     4021 atgtgaagca gagtaaagca acgtggagtt ggataaatgc tctggctcaa atagtggtat
     4081 caggattcac cggtgttatt ggtggcctga tcagcatcga aagtggattc agtatttaca
     4141 tgattctcgc gacagcgggg attagtggtg cgatgggttc ggttgcactg acgtacttct
     4201 gggaacgact gacaggggtg aaaaatgcaa aatcttaatc ctcagcgtaa agccttcctc
     4261 gatatgttgg cgtggtcaga aggaacggat aacgggcgac aaccgacacg taaccacggt
     4321 tatgatgtta ttgttggcgg cgaactgttt actgattact ccgatcaccc tcgcaaactt
     4381 gtcacgctac accccaaact caaatcaaca gccgccggac gttatcagct tctttcacgc
     4441 tggtgggatg cttaccgtaa acagcttggt ttgaaagact tctcccccaa aagccaggac
     4501 gcagtggcat tacagcagat taaagagcgt ggtgctttac cgatgattga tcgcggtgat
     4561 atccgtcagg caatcgaccg ttgcagcaat atctgggctt cactgccggg cgctggttat
     4621 ggtcagttcg agcataaggc tgacagcctg attgcaaaat tcaaagaagc tggcggaacg
     4681 ctcagagaga gtgaggtatg agcagaataa ccgcgattat ctccgctctg gttatctgcg
     4741 tcatcgtctg cctttcatgg gcggttaatc attaccgcga taacgcgatt acctacaaag
     4801 cccagcgcga caaaaatgcc agagaactga cgctggcgaa cgcggcaatt actgacatac
     4861 agatgcgtca gcgtgatgtt gctgcgctcg atgcaaaata cacgaaggag ttagctgatg
     4921 cgaaagctga aaatgatgct ctgcgtgatg atgttgccgc tggtcgtcgt cggttgcaca
     4981 tcaaagcagt ctgtcagtca gtgcgtgaag ccaccaccgc ctccggcgtg gataatgcag
     5041 cctccccccg actggcagac accgctgaac gggattattt caccctcaga gagaggctga
     5101 tcactatgca aaaacaactg gaaggaaccc agaagtatat taatgagcag tgcagataga
     5161 gctgcccata tcgatgggca actcatgcaa ttattgtgag caatacacac gcgcttccag
     5221 cggagtataa atgcctaaag taataaaacc gagcaatcca tttacgaatg tttgctgggt
     5281 ttctgtttta acaacatttt ctgcaccacc acaaattttt gctgcatcga cagttttctt
     5341 ctgcccaatt cccgaaacga agaaatgatg ggtgatggtt tcctttggtg ttactgctgt
     5401 aggtttgttt ccaacagtaa acgtctgttg agcacatcct gtaataagca ttgccagagc
     5461 ggcagaaaac aacatttttt tcatcttatt atcctgcatt gttaaaaacg gcagaatcct
     5521 atgtgacaac aattaaacga tagttaaatg gattgatgaa aattaaaact acacaggtgg
     5581 gctcagacta ttggaggaag ttggggacac tcagaatcct gtggaatgaa ataaaccggt
     5641 ctatccgtcc attacccttt tagctgcgct gtatcgtcgc cgtattcccg cattaaccat
     5701 gaccgtagcc cgacggggaa ttccttctgc gtgagtgtgc gggaataatt aaaaacgatg
     5761 cacaccgggt ttttaccgcg ttaatgattc gcgggtttat cccggtgcga tggtggaaga
     5821 aacaggaagc tgtattacag aaagtgctac tactgtatcc cgatgcgatg tatgtaatgt
     5881 gagtcagata atggcacagg atgtggtgat gtggcagtct ggaacacagg atatattgtc
     5941 agaataagac ccgtaggaat aaaaatgaaa agacgccttt tactactttt tctgttatct
     6001 gtcctggcag tgggatgctc gcagcaaaaa gctgatgagc cccggcaatt agtgacggtg
     6061 tatccacgat atccggaata tgctgcagca aattatatca aggggctggt tgaggttaag
     6121 ttcgatattg gtgctgatgg gactgtgaca cggatcgttt ttctccgctc agagcctcat
     6181 aatttgtttc gtgatgaagt ggtgaaggcc atggcgaaat ggcgatttga aaagaatcgc
     6241 ccctgtcagg gagtgaagag acaatttatc tttacgccgt cacgtccttg atgcttccag
     6301 gtagagaggg gctggaagca ggagaaaaat gaaagagcca gcggttatat ttttgtcatg
     6361 gctgacgagg aatgatggaa gaaggcgttg tatgccacac aacgcctcac tgttcatttc
     6421 ttctttttct ctggtggaac ccgatgaata agagttgcac tggtttccga tgagatggcg
     6481 atatactcgg gcaaagtatg ctggcagttt tccaactggt caaaaatacc tgctctcgtc
     6541 tgttgcaatg cctgcagcat gcggcggcaa tgcgccttgc ttttactaac catctttcct
     6601 tcctctatca gtcgctgcgt gaactcatca tggaatacca ggtaaatgcg gatgttatcg
     6661 gttttggcta cgcagcatag tacaaaacgg acaggtgcat cccgggacgg gggaggcgtc
     6721 acatgtccct gtgatggttg ttccgggtaa tgtactgtgt ggggcataaa aatgtccgat
     6781 aattttactt tctaccgcag ttagttgatt cgttggtcct ggtagcacat tgggcgagga
     6841 tttaaatgcc aggcaactga aggatgatgt cgcaagggag atagcgagaa tatttctgat
     6901 tttcatttga tgatgcctct gtgtgaaatg acggtaaacg acgcacttgt gccggcacat
     6961 aatagcaagc accataatag atcagattcg attcttgctg taagtgataa ttattctcgt
     7021 tttcgggtcc tttccgtcga tccaacaggt tacggggcgg cgacctcgcg ggttttcgct
     7081 atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgccgtaa cttaatgttt
     7141 ttatttaaaa cactccctga aaagaaagga aacgacaggt gctgaaaacg ggctttttgg
     7201 cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tcaacaaaaa
     7261 gcagctggct gacattttcg gcgcgagtat ccgtactatt cagaactggc aggagcaggg
     7321 aatgcccgtt ctgcgaggcg gtgggaaggg taatgaggtg ctttatgact ctgccgacgt
     7381 cataaaatgg tatgccgaaa gggatgctga aattgagaac gaaaagctgc gccgggaggt
     7441 tgaagaactg cgacaggcca gcgagacaga tctccagcca gggactattg agtacgaacg
     7501 ccatcgactt acgcgtgcgc aggccgacgc acaggagctg aaaaatgcca gagactccgc
     7561 tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg cggatcgcag gtgaaattgc
     7621 cagtattctc gacgggatcc ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg
     7681 acatgttgat ttcctgaaac gggatatcat caaagccatg aacaaagcag ccgcgctgga
     7741 tgaactgata ccggggttgc tgagtgaata tatcgaacag tcaggttaac aggctgcggc
     7801 attttgtccg cgccgggctt cgctcactgt tcaggccgga gccacagacc gccgttgaat
     7861 gggcggatgc taattactat ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa
     7921 cactgccctt tcagcgggcc atcatgaatg cgatgggcag cgactacatc cgtgaggtga
     7981 atgtggtgaa gtctgcccgt gtcggttatt ccaaaatgct gctgggtgtt tatgcctact
     8041 ttatagagca caagcagcgc aacaccctta tctggttgcc gacggatggt gatgccgaga
     8101 actttatgaa aacccacgtt gagccgacca tccgcgatat tccgtcgctg ctggcgctgg
     8161 ctccgtggta tggcaaaaag caccgggata acacgctcac tatgaagcgt ttttccaatg
     8221 gtcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcggtgg
     8281 atgtggcggg ttatgatgaa cttgctgcct ttgatgagga tattgaacag gaaggctctc
     8341 cgacgttcct tggcgacaaa cgtattgaag gctcggtctg gccaaagtcc atccgtggct
     8401 ccacgcccaa agtgagaggc acctgtcaga ttgagcgtgc agccagtgaa tccccgcatt
     8461 gtatgcgttt tcatgttgcc tgcccgcact gcggggagga gcagtacctt aaatttggcg
     8521 ataaagagac gccgtttggc ctcaaatgga cgccggatga tccctccagc gtgttttatc
     8581 tctgcgaaca taatgcctgc gtcatccgcc agcaggagct ggacttcact gatgcccgtt
     8641 atatctgcga aaagaccggg atctggaccc gtgatggcat tctctggttt tcgtcatccg
     8701 gtgaagagat tgagccgccg gacagcgtga cctttcacat ctggacggcg tacagcccgt
     8761 tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac gaaaggagat acgggaaaac
     8821 gtaaaacctt cgtgaacacc acgctcggtg agacatggga agcgaaaatc ggtgaacgtc
     8881 cggatgctga agtgatggca gagcggaaag agcattattc agcgcccgtt cctgaccgtg
     8941 tggcttacct gactgccggt atcgactccc agctggatcg ctacgaaatg cgcgtatggg
     9001 gatgggggcc gggtgaggaa agctggctga ttgaccggca gattattatg ggccgccacg
     9061 acgatgaaca gacgctgctg catgtggatg aggccatcaa taaaacctat acccgccgga
     9121 atggtgcaga aatgtcggta tcccgtatct gctgggatat tggcgggatt gacccgacca
     9181 tcgtgtatga acgctcgaaa aaacatgggc tgttccgggt gatccccatt aaaggggcat
     9241 ccgtctacgg aaagccggtg gccagcatgc cacgtaagcg aaacaaaaac ggggtttacc
     9301 ttacggaaat cggtacggat accgcgaaag agcagattta taaccgcttc acactgacgc
     9361 cggaagggga tgaaccgctt cccggtgccg ttcacttccc gaataacccg gatatttttg
     9421 atctgaccga agcgcagcag ctgactgctg aagagcaggt cgaaaaatgg gtggatggca
     9481 ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga ggcgctcgac tgcttcgttt
     9541 atgcgctggc ggcgctgcgc atcagtattt cccgctggca gctggatctc agtgcactgc
     9601 tggcgagcct gcaggaagag gatggtgcag caaccaacaa gaaaacactg gcagattacg
     9661 cccgtgcctt atccggagag gatgaatgac gcgacaggaa gaacttgccg ctgcccgtgc
     9721 ggcactgcat gacctgatga caggtaaacg ggtggcaacg gtacagaaag acggacggcg
     9781 agtggagttt acggccactt ccgtgtctga cctgaaaaaa tacattgcgg agctggaggt
     9841 gcagaccggc atgacacagc gacgcagggg acctgcagga ttttatgtat gaaaacgccc
     9901 accattccca cccttctggg accggacggc atgacatcgc tgcgtgaata tgccggttat
     9961 cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt ggaacccacc gggtgaaagt
    10021 gtggatgcag ccctgctgcc caactttacc cgtggcaatg cccgcgcaga cgatctggta
    10081 cgcaataacg gctatgctgc caacgccatc cagctgcatc aggatcatat cgtcgggtct
    10141 tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagacgcc
    10201 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc
    10261 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
    10321 cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt
    10381 ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc
    10441 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat
    10501 tacgtcagtg aggacggcta tcctggctgg atgccgcaga aatggacatg gataccccgt
    10561 gagttaccgg gcggtcgtgc ttcgttcatt cacgtctttg aacccgtgga ggacgggcag
    10621 acccgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
    10681 cagaacacgc agctgcagag cgccattgtg aaggcgatgt atgccgccac catcgagagt
    10741 gagctggata cgcagtcagc gatggatttt attcttggcg cgaacagtaa ggagcagcgg
    10801 gacaggctga ccggctggat tggtgaaatt gccgcgtatt acgccgcagc accggtccgg
    10861 ctgggaggcg caaaagtgcc tcacctgatg ccgggtgact cactgaacct gcagacggct
    10921 caggacacgg ataacggcta ctccgtgttt gagcagtcac tgttgcggta tatcgctgcc
    10981 gggctgggtg tctcgtatga gcagctttcc cggaattacg cccagatgag ctactccacg
    11041 gcacgggcca gtgcgaacga gtcgtgggcg tactttatgg ggcggcgaaa attcgtcgca
    11101 tcccgtcagg cgagccagat gtttctgtgc tggctggaag aggccatcgc tcgccgcgtg
    11161 gtgacgttac cttcaaaagc gcgcttcagt tttcaggaag cccgaagtgc ctgggggaac
    11221 tgcgactgga taggctccgg tcgtatggcc atcgatggtc tgaaagaagt acaggaagcg
    11281 gtgatgctga tagaagccgg actgagtacc tacgagaaag agtgcgcaaa acgcggtgac
    11341 gactatcagg aaatttttgc ccagcaggtc cgtgaaacga tggagcgccg tgcagccggt
    11401 cttaaaccgc ccgcctgggc ggctgcggca tttgaatccg ggctgcgaca atcaacagag
    11461 gaggagaaga gtgacagcag agctgcgtaa tctcccgcat attgccagta tggcctttaa
    11521 tgagccgctg atgcttgaac ccgcctatgc gcgggttttc ttttgtgcgc ttgcaggcca
    11581 gcttgggatc agccgcctga cggatgcggt atccggcgac agcctgactg ccggagaggc
    11641 acccgcggcg ctggcgttat ccgttgatga tgacggacca cgacaggccc gcagttatca
    11701 ggtcatgaac ggcatcgccg tgctgccggt gtccggcacg ctggtcagtc ggacgcgagc
    11761 gctgcagccg tattcgggga tgaccggtta caacggcatt atcgcccgtc tgcaacaggc
    11821 tgccagcgac ccgatggtgg acggcattct gctcgatatg gatacgccag gcgggatggt
    11881 ggcaggggca tttgactgcg ctgacatcat cgcccgtgtg cgtgacataa agccggtatg
    11941 ggcgctggcc aacgacatga actgcagtgc aggtcagttg cttgccagcg ccgcctcccg
    12001 acgtctggtc acgcagaccg cccggacagg ctccatcggc gtcatgatgg ctcacagtaa
    12061 ttacggtgct gcgctggaga aacagggcgt ggaaatcacg ctgatttaca gcggcagcca
    12121 taaggtggat ggcaacccct acagccatct accggatgat gtccgggaaa cactgcagtc
    12181 ccggatggat gcaacccgcc ggatgtttgc acagaaggtg tcggcatata ccggcctgtc
    12241 cgtgcaggct gtgctggata ccgaggctgc agtgtacagc ggtcaggagg ccattgatgc
    12301 cggactggct gatgaacttg ttaacagtac cgatgcgatc accgtcatgc gtgatgcact
    12361 ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc aaagagactc aatcaacaac
    12421 tgtttcagcc actgcttcgc aggctgacgt tactggcgtg gtgccagcga cggagggcga
    12481 gaacgccagc gctgcgcagc cggacgtgaa cgcgcagatc accgcagcgg ttgcggcaga
    12541 aaacagtcgc attatgggga tcctcaactg tgaggaagct cacggacgcg aagaacaggc
    12601 acgcgtgctg gccgaaaccc ctggtatgac cgtggaaacg gcccgccgca ttctggcagt
    12661 tgcaccacag agtgcacagg cgcgcagtga cactgcgctg gatcgtctga tgcagggggc
    12721 accggcaccg ctggctgcag gtaacctggc atctgatgcc gttaacgatt tgctgaacac
    12781 accagtgtaa gggatgttta tgacgagcaa agaaaccttt acccattacc agccgctggg
    12841 caacagtgac ccggctcata ccgcaaccgc gcccggcgga ttgagtgcga aagcgcctgc
    12901 aatgaccccg ctgatgctgg acacctccag ccgtaagctg gttgcgtggg atggcaccac
    12961 cgacggtgct gccgttggca ttcttgcggt tgctgctgac cagaccagca ccacgctgac
    13021 gttctacaag tccggcacgt tccgttatga ggatgtgctc tggccggagg ctgccagcga
    13081 cgagacgaaa aaacggaccg cgtttgccgg aacggcaatc agcatcgttt aactttaccc
    13141 ttcatcacta aaggccgcct gtgcggcttt ttttacggga tttttttatg tcgatgtaca
    13201 caaccgccca gctgctggcg gcaaatgagc agaaatttaa gtttgatccg ctgtttctgc
    13261 gtctcttttt ccgtgagagc tatcccttca ccacggagaa agtctatctc tcacaaattc
    13321 cgggactggt aaacatggcg ctgtacgttt cgccgattgt ttccggtgag gttatccgtt
    13381 cccgtggcgg ctccacctct gaatttacgc cgggatatgt caagccgaag catgaagtga
    13441 atccgcagat gaccctgcgt cgcctgccgg atgaagatcc gcagaatctg gtggacccgg
    13501 cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga cgaagagctg gccattgctc
    13561 aggtcgaaga gatgcaggca gtttctgccg tgcttaaggg caaatacacc atgaccggtg
    13621 aagccttcga tccggttgag gtggatatgg gccgcagtga ggagaataac atcacgcagt
    13681 ccggcggcac ggagtggagc aagcgtgaca agtccacgta tgacccgacc gacgatatcg
    13741 aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat cgtgttcgat ccgaaaggct
    13801 gggcgctgtt ccgttccttc aaagccgtca aggagaagct ggatacccgt cgtggttcta
    13861 attccgagct ggagacagcg gtgaaagacc tgggtaaagc ggtgtcctat aaggggatgt
    13921 atggcgatgt ggccatcgtc gtgtattccg gacagtacgt ggaaaacggc gtcaaaaaga
    13981 acttcctgcc ggacaacacg atggtgctgg ggaacactca ggcacgcggt ctgcgcacct
    14041 atggctgcat tcaggatgcg gacgcacagc tcgaaggcat taacgcctct gcccgttacc
    14101 cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt caccatgatt cagtcagcac
    14161 cgctgatgct gctggctgac cctgatgagt tcgtgtccgt acaactggcg taatcatggc
    14221 ccttcggggc cattgtttct ctgtggagga gtccatgacg aaagatgaac tgattgcccg
    14281 tctccgctcg ctgggtgaac aactgaaccg tgatgtcagc ctgacgggga cgaaagaaga
    14341 actggcgctc cgtgtggcag agctggaaga ggagcttgat gacacggatg aaactgccgg
    14401 tcaggacacc cctctcagcc gggaaaatgt gctgaccgga catgaaaatg aggtgggatc
    14461 agcgcagccg gataccgtga ttctggatac gtctgaactg gtcacggtcg tggcactggt
    14521 gacgctgcat actgatgcac ttcacgccac gcgggatgaa cctgtggcat ttgtgcttcc
    14581 gggaacggcg ttccgtgtct ctgccggtgt ggcagccgaa atgacagaac gtggcctggc
    14641 cagaatgcaa taacgggagg cgctgtggct gatttcgata acctgttcga tgctgccatt
    14701 gtccgcgccg atgaaacgat acgcgggtac atgggaacgt cagccaccat gacatccggt
    14761 gagcagtccg gcgcagtaat acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc
    14821 ggacagggcg tgcgcgttga aggctccagc ccgtccctgt ttgtccggac tgatgatgtg
    14881 cggcagctgc ggcgcggcga cacgctgacc atcggtgagg aaaacttctg gatagaccgg
    14941 atttcgacgg atgatggcgg aagctgtcat ctctggcttg ggcgtggcgt gccgcctgcc
    15001 gttaaccgtc gtcgctgaag gggggatgta tggccataaa aggtcttgag caggccgttg
    15061 aaaacctcag ccgtatcagc agaacggcgg tgcccggtgc cgccgcaatg gccattaacc
    15121 gcgttgcttc atccgcgata tcgcagtcgg cgtcacaggt tgcccgtgag acaaaggtac
    15181 gccggaaact ggtaaaggaa agggccaggc tgaaaagggc cacggtcaaa aatccgcagg
    15241 ccagaatcaa ggttaaccgg ggggatttgc ccgtaatcag gctgggtaac gcgcgggttg
    15301 tcctgtcccg acgcaggcgt cgtaaaaagg ggcagcgttc atccctgaaa ggtggcgaca
    15361 gcgtgcttgt ggtgggaaac cgtcgtattc ccggcgcgtt tattcagcaa ctgaaaaatg
    15421 gccgatggca tgtcatgcag cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga
    15481 aaatcccgat ggcagtgccg ctgaccacgg cgtttaaaca gaatattgag cggatacggc
    15541 gtgaacgtct tccgaaagag ctgggctatg cgctgcagca tcaactgagg atggtaataa
    15601 agcgatgaaa catacagaac tccgtgcagc cgtactggat gcactggaaa aacatgacac
    15661 cggggcgacg ctttttgatg gtcgccccgc tgtttttgat gaggaggatt ttccggcagt
    15721 tgccgtttat ctcaccggcg ctgaatacac gggcgaagag ctggacagcg atacctggca
    15781 ggcggagctg catattgaag ttttcctgcc tgctcaggtg ccggattcag agctggattc
    15841 gtggatggag tcccggattt atccggtgat gagcgatgtc ccggcactgt cagatttgat
    15901 caccagtatg gtggccagtg gctatgacta ccggcgcgac gatgatgcgg gcctgtggag
    15961 ttcagccgat ctgacttatg tcattaccta tgaaatgtga ggacgatatg cctgtaccaa
    16021 atccagtaat gccggtgaaa ggggccggga ccaccctgtg ggtttataag gggaacggtg
    16081 acccttatgc gaacccgctt tcagacgttg actggtcgcg tctggctaaa gttaaagacc
    16141 tgacgcccgg cgaactgagc gctgagtcct atgacgacag ctatctcgat gatgaagatg
    16201 cggactggac tgcgaccggg caggggcaga aatctgccgg agataccagc ttcacgctgg
    16261 cgtggatgcc cggagagcag gggcagcagg cgctgctggc gtggtttaat gaaggggata
    16321 cccgtgccta taaaatccgc ttcccgaacg gcacggtcga tgtgttccgc ggctgggtca
    16381 gcagtatcgg taaggcggtg acggcgaagg aagtgatcac ccgcacggtg aaagtcacca
    16441 acgtgggacg tccgtcgatg gcagaagatc gcagcacggt gacggcgaca accggcatga
    16501 cggtgacacc cgccagtgct tccgtagtga aagggcagag cactacgctg accgtggcat
    16561 tccagccgga gggcgcaacc gacaagagct tccgtgcggt gtctgcggat aaaacaaaag
    16621 ccaccgtgtc ggtcagcggt atgaccatca ccgtgaacgg cgttgctgca ggcaaggtca
    16681 acattccggt tgtatccggt aatggtgagt ttgctgcggt tgcagaaatc accgtcaccg
    16741 ccagttaatc cggagagtca gcgatgttcc tgaaaaccga atcatttgaa tataacggcg
    16801 tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat tgagcatctt gccctgatga
    16861 aacggcaggc agaaccggcg ggatccgaca gcaaccggca ggttactgtg gaagacgtca
    16921 tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca taaccatccg aagaagacgc
    16981 agatgccgtc catgaatgaa gccgttaaac agattgagca ggaagtgctt accacctggc
    17041 ccacagaggc aatttctcat gctgaaaacg tggtgtaccg gctgtccggt atgtatgagt
    17101 ttgtggtgaa taatgccccc gaacaggcag aggacgccgg gcctgcagag cctgtttctg
    17161 cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa ctggcgcgtg agatggggcg
    17221 acccgactgg cgtgccatgc ttgccgggat gtcatccacg gagtatgccg actggcaccg
    17281 cttttacagt acccattatt ttcatgatgt tctgctggat atgcactttt ccgggctgac
    17341 gtacaccgtg ctcagcctgt ttttcagcga tccggatatg catccgctgg atttcagtct
    17401 gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat gtgctgatgc agaaagcggc
    17461 agggcttgcc ggaggtgtcc gctttggccc ggacgggaat gaagttatcc ccgcttcccc
    17521 ggatgtggcg gacatgacgg aggatgacgt aatgctgatg acagtatcag aagggatcgc
    17581 aggaggagtc cggtatggct gaaccggtag gcgatctggt cgttgatttg agtctggatg
    17641 cggccagatt tgacgagcag atggccagag tcaggcgtca tttttccggt acggaaagtg
    17701 atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag ccgacaggcg ctggctgcac
    17761 agaaagcggg gatttccgtc gggcagtata aagccgccat gcgtatgctg cctgcacagt
    17821 tcaccgacgt ggccacgcag cttgcaggcg ggcaaagtcc gtggctgatc ctgctgcaac
    17881 agggggggca ggtgaaggac tccttcggcg ggatgatccc catgttcagg gggcttgccg
    17941 gtgcgatcac cctgccgatg gtgggggcca cctcgctggc ggtggcgacc ggtgcgctgg
    18001 cgtatgcctg gtatcagggc aactcaaccc tgtccgattt caacaaaacg ctggtccttt
    18061 ccggcaatca ggcgggtctg acggcagatc gtatgctggt cctgtccaga gccgggcagg
    18121 cggcagggct gacgtttaac cagaccagcg agtcactcag cgcactggtt aaggcggggg
    18181 taagcggtga ggctcagatt gcgtccatca gccagagtgt ggcgcgtttc tcctctgcat
    18241 ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa gctgaccaca gacccgacgt
    18301 cggggctgac ggcgatggca cgccagttcc ataacgtgac ggcggagcag attgcgtatg
    18361 ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt gcaggcggcg aacgaggccg
    18421 caacgaaagg gtttgatgac cagacccgcc gcctgaaaga gaacatgggc acgctggaga
    18481 cctgggcaga caggactgcg cgggcattca aatccatgtg ggatgcggtg ctggatattg
    18541 gtcgtcctga taccgcgcag gagatgctga ttaaggcaga ggctgcgttt aagaaagcag
    18601 acgacatctg gaatctgcgc aaggatgatt attttgttaa cgatgaagcg cgggcgcgtt
    18661 actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc cgcccgaaag aaggctgaac
    18721 agcagagtca acaggacaaa aatgcgcagc agcagagcga taccgaagcg tcacggctga
    18781 aatataccga agaggcgcag aaggcttacg aacggctgca gacgccgctg gagaaatatg
    18841 ccgcccgtca ggaagaactg aataaggccc tgaaagacgg aaaaatcctg caggcagatt
    18901 acaacacgct gatggcggca gcgaaaaagg actatgaaac gacactgaaa aagccgaagc
    18961 agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga cagtgctcat gctgccctgc
    19021 tgacgcttca ggctgaactc cggacgctgg agaagcatgc cggagcaaat gagaaaatca
    19081 gccagcagcg ccgggatttg tggaaggcgg agagtcagtt cgcggtactg gaggaggcgg
    19141 cgcaacgtcg ccagctgtct gtacaggaga aatccctgct ggcgcataaa gatgagacgc
    19201 tggagtacaa acgccagctg gctgcacttg gcgacaaggt tacgtatcag gagcgcctga
    19261 acgcgctggc gcagcaggcg gataaattcg cacagcagca acgggcaaaa cgggccgcca
    19321 ttgatgcgaa aagccggggg ctgactgacc ggcaggcaga acgggaagcc acggaacagc
    19381 gcctgaagga acagtatggc gataatccgc tggcgctgaa taacgtcatg tcagagcaga
    19441 aaaagacctg ggcggctgaa gaccagcttc gcgggaactg gatggcaggc ctgaagtccg
    19501 gctggagtga gtgggaagag agcgccacgg acagtatgtc gcaggtaaaa agtgcagcca
    19561 cgcagacctt tgatggtatt gcacagaata tggcggcgat gctgaccggc agtgagcaga
    19621 actggcgcag cttcacccgt tccgtgctgt ccatgatgac agaaattctg cttaagcagg
    19681 caatggtggg gattgtcggg agtatcggca gcgccattgg cggggctgtt ggtggcggcg
    19741 catccgcgtc aggcggtaca gccattcagg ccgctgcggc gaaattccat tttgcaaccg
    19801 gaggatttac gggaaccggc ggcaaatatg agccagcggg gattgttcac cgtggtgagt
    19861 ttgtcttcac gaaggaggca accagccgga ttggcgtggg gaatctttac cggctgatgc
    19921 gcggctatgc caccggcggt tatgtcggta caccgggcag catggcagac agccggtcgc
    19981 aggcgtccgg gacgtttgag cagaataacc atgtggtgat taacaacgac ggcacgaacg
    20041 ggcagatagg tccggctgct ctgaaggcgg tgtatgacat ggcccgcaag ggtgcccgtg
    20101 atgaaattca gacacagatg cgtgatggtg gcctgttctc cggaggtgga cgatgaagac
    20161 cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg gtcccttctg taagaaaggt
    20221 gcgctttggt gatggctatt ctcagcgagc gcctgccggg ctgaatgcca acctgaaaac
    20281 gtacagcgtg acgctttctg tcccccgtga ggaggccacg gtactggagt cgtttctgga
    20341 agagcacggg ggctggaaat cctttctgtg gacgccgcct tatgagtggc agcagataaa
    20401 ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg cgtgttgagt tcagcgcaga
    20461 gtttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa tgaatgcacc
    20521 cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg acctgacaga ggtcggtgga
    20581 gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac ctggcagggg
    20641 cgacagtatc agccgtatcc cattcagggg agcggttttg aactgaatgg caaaggcacc
    20701 agtacgcgcc ccacgctgac ggtttctaac ctgtacggta tggtcaccgg gatggcggaa
    20761 gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg
    20821 gatgcggtga acttcgtcaa cggaaacagt tatgccgatc cggagcagga ggtgatcagc
    20881 cgctggcgca ttgagcagtg cagcgaactg agcgcggtga gtgcctcctt tgtactgtcc
    20941 acgccgacgg aaacggatgg cgctgttttt ccgggacgta tcatgctggc caacacctgc
    21001 acctggacct atcgcggtga cgagtgcggt tatagcggtc cggctgtcgc ggatgaatat
    21061 gaccagccaa cgtccgatat cacgaaggat aaatgcagca aatgcctgag cggttgtaag
    21121 ttccgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa
    21181 tcccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga
    21241 gtcgtgtggc ttcgtggtga gaacgccgaa gggggaaaga tattttccct gcgtgaatat
    21301 ctccggtgag ccggaggcgt atttccggat gtcgccggag gactggctgc gggcagagat
    21361 gcagggtgag attgtggcgc tggtccacag ccaccccggt ggtctgccct ggctgagtga
    21421 ggctgacagg cggctgcagg tgcagagcga tttgccgtgg tggctggtct gccggggtga
    21481 gattcataaa ttccgctgtg tgccgcatct taccgggcgg tgctttgagc acggggtgac
    21541 ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt
    21601 tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata atctggaggc
    21661 cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg
    21721 ttttggttca tcggtgccga atcatgccgc catttactgt ggtgatggcg agctgctgca
    21781 ccatattcct gaacaactga gcaaacgaga gaggtatacc gacaaatggc agcgacgcac
    21841 acactccctc tggcgtcacc gggcatggcg cgcatctgcc tttacgggga tttacaacga
    21901 tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg ggcgctggcc
    21961 acacagctcc cggcgtttcg tcagaaactg agcgacggct ggtatcaggt acggattgcc
    22021 gggcgtgata caggtgaaac ggaattatca gcccgtctta atgaaccgct ggcaaatggt
    22081 gctgtgatcc atatcgtgcc gcgtctggca ggagccaaaa gtggcggtgt gtttcaggct
    22141 gtgctggggg cggctgttat ggcggttgct atatggatgc cgggggtagg aattatggcg
    22201 agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc acagatgctg
    22261 gcccctaaac ccagaacccc ccgcacacag acaacggata acggcaaaca gaacacctat
    22321 ttttcttcac tggataatat ggttgcccag ggcaatgttc tgcccgttct gtacggtgaa
    22381 atgcgcgtgg gatcacgtgt ggtatctcag gagatcagca cagcagatga aggtgatggt
    22441 ggtcaggttg tggtgattgg tcgctgatga aaaacgtttt atgtgaaacc gcctccgggc
    22501 ggttttatcg tttatggagc atgacgaatg ggtaaaggca gcagtaaggg gcataccccg
    22561 cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga tgccatcagc
    22621 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
    22681 gtgctggaca gtgaggggaa taccaatata tccggcgtca cggtggtgtt ccgggccggt
    22741 gagcaggagc agtcaccgcc ggagggattt gaatcctccg gctccgagac ggtgctgggt
    22801 acggaagtga aatatgacac gccgatcacc cggaccatca cgtcggcaaa catcgaccgt
    22861 ctgcgcttta ccttcggtgt acaggcactg gtggaaacca cctcaaaggg agacaggaat
    22921 ccgtcggaag ttcgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa
    22981 gacatcacca ttaagggcaa aaccacctcg cagtatctgg cctcggtggt ggtggataac
    23041 ctgccgccgc gcccgtttaa tatccggatg cgcaggatga cgccggacag caccacagac
    23101 cagctgctga acaaaacgct ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc
    23161 tacccgaaca cggcactggt tggcgtgcag gtggactcgg agcagttcgg cagccagcag
    23221 gtgagccgta attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg
    23281 cagacgcggc aatacagcgg tatctgggac ggaacgttaa aaccggcata cagcaacaac
    23341 atggcctggt gtctgtggga tatgctgacc catccgcgct acggcatggg gaaacgtctt
    23401 ggtgcggcgg atgtggataa atgggcgctg tatgtcatcg gccagtactg cgaccagtcg
    23461 gtgccggacg gctttggcgg cacggagccg cgcattacct gtaatgcgta cctgaccaca
    23521 cagcgcaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta
    23581 tggaacgggc agacgctgac gttcgtgcag gaccgaccat cagataaggt gtggacctat
    23641 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc
    23701 ctgaaggacc gccataatgc cgttgaggtg aactggattg acccggataa cggctgggag
    23761 acggcgacag agctcgtgga ggatacgcag gccattgccc gttacggccg taacgtcacg
    23821 aagatggatg cctttggctg taccagccgg gggcaggcac accgcgccgg gctgtggctg
    23881 attaaaacgg agctgctgga aacgcagacc gtggacttca gcgtgggtgc tgaagggctt
    23941 cgccatgtac cgggggatgt cattgaaatc tgcgatgatg actatgccgg tatcagcacc
    24001 ggtggtcgtg tgctggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc
    24061 acgctgccat cctccggtac cacgctgata agcctggttg acggaagtgg caatccggtc
    24121 agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg tgttcctgac
    24181 ggtgttgctg aatacagcgt atgggggctg aagctgccga cgctgcgcca gcgactgttc
    24241 cgctgcgtga gtatccgtga aaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat
    24301 gtgccggaaa aagagaccat cgtggataac ggggcgcact ttgacggcga acagagtggc
    24361 acggtgaatg gtgtcacgcc gcccgcggtg cagcacctga ccgcagaagt caccgcagac
    24421 agcggggaat atcaggtgct ggcgcgatgg gacacaccga aggtggtgaa gggggtgagc
    24481 tttatgcttc gcctgaccgt ggcagcggat gacggcagtg agcggctggt cagcacggcc
    24541 aggacgacgg aaaccacata ccgcttcagg caactggcgc tggggaacta caggctgaca
    24601 gtccgggcgg taaatgcgtg ggggcagcag ggggatccgg cgtcggtatc gttccggatt
    24661 gccgcaccgg cagcgccatc gcggattgag ctgacgccgg gctattttca gataaccgca
    24721 acgccgcatc ttgccgttta tgatccgacg gtacagtttg agttctggtt ctcggaaaag
    24781 cggattgcgg atatcaggca ggttgaagcc agcgcgcgtt atcttggtac ggcgctgtac
    24841 tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attattttta cattcgcagt
    24901 gtgaacaccg ttggcaaatc ggcatttgtg gaggctgttg gccagccgag tgatgatgca
    24961 tccggctatc tggatttttt caaaggagag atagggaaaa cccatctggc tcaggagttg
    25021 tggacgcaga ttgataacgg tcagcttgcg cctgacctgg ctgaaatcag gacgtccatt
    25081 acggatgtca gcaatgaaat cacgcagacc gtcaataaga aactggaaga ccagagtgca
    25141 gcgatccagc agatacagaa ggttcaggtt gatacaaata ataacctgaa cagcatgtgg
    25201 gctgtgaagc tgcagcagat gcaggacgga cgcctttata tcgcgggtat tggtgccggt
    25261 attgagaata cccctgacgg tatgcagagt caggtgctgc tggcggcgga caggattgcg
    25321 atgattaatc ctgcgaatgg caatacaaag ccgatgtttg ttgggcaggg cgatcagata
    25381 ttcatgaacg aagtgttcct gaaatatctg acggctccca ccattaccag cggtggcaat
    25441 cccccaacgt tttcactgac acctgatggt cgactttctg cgagaaatgc ggatatcagc
    25501 ggtaacgtga atgcgaactc cgggacgctc aataatgtca cgattaacca gaactgtcgg
    25561 attctgggaa aactgtcagc taaccagatt gaaggtgata ttgtcaaaac ggtgggaaaa
    25621 gcctttccga gaaatggcag ttatgccagc ggtacaataa cggtcactgt gtacgatgac
    25681 caggcttttg accgtcagat agtcgtcccc cccgttctgt ttcgcggtgg taagcatgaa
    25741 aacttcaaca gcaacaacca acagtcatac tggtattcaa cctgtaagct gcaggtgctg
    25801 aagaacggac aggaaatctt tcagcaaccc gcgacggatg tcagcagggt attttcatca
    25861 gtcattgata tgcctgccgg acacggccat gtcaccctga ctttcaatgt ttcttcatat
    25921 ggtgcaaata actggacgcc aacgaccagt atcagcgacc ttcttgttgt cgtgatgaag
    25981 aaatcaacag ccggcatcag tatcagttga attttaaaac ccaaattcgg gcaccagaaa
    26041 tggtgccttt tttatttgtg gagtgaatat ggcagtacag atttcaggtg tgctgaaaga
    26101 cggtgcagga aaaccgatac agaactgcac cattcagctc aaagcaaaac gtaacagcac
    26161 cacagttgtg gtgaacacgg tggcctcaga aaatccggat gaagccggac gttacagcat
    26221 ggatgttgag tatggccagt acagcgtcgc cctgctggtt gaaggttttc cgccttcaca
    26281 tgccgggacc atcaccgtgt atgaggattc taagccgggg acattgaatg attttctggg
    26341 cgctgcaaca gaagatgatg tttgtccgga ggcactgtat cgttttgaaa agatggtgga
    26401 agaggcggca cgcaacgctg aagccgcctc tcagagcgca gcggcagcaa agaaatcaga
    26461 aacagcagcg gcatcgtcca ggaacgcggc gaaaacatca gagacgaatg caggtaacag
    26521 cgcgaaagcg gcagcttctt caaaaacagc cgcacaaaac gcagcaacag cggcagaacg
    26581 ttcagagaca aatgcccgtg cgtcagaaga agcctccgca gacagtgaag aggcttcccg
    26641 ccgtaatgca gagtcagccg ctgaaaatgc cggagtcgcc accacaaaag cgcgggaggc
    26701 cgcagcagac gcaacaaagg ccgggcagaa aaaggatgag gctctgtcgg cagcgacacg
    26761 agctgaaaag gcggcagacc gcgcagaagc cgcagcggaa gtgactgcag agccctgtgc
    26821 gaatatagtg ccgccgctgc ctgatgtgtg gataccgttt aacgattcac tggatatgat
    26881 tacgggtttt tcgccatctt ataaaaagat agttattggt gacgatgaaa taacaatgcc
    26941 aggcgacaag attgttaagt ttaaacgtgc ttcaacagca acgtatatta ataagtccgg
    27001 ccaactcaag cttgctgaag ttgacgaacc gcgatttgag cgcgatggct tattgattga
    27061 aggacagagg acaaattatc tgaggaactc aaataaacca gactcatgga ctgttcattc
    27121 cgcactgaat aaaacatttg gcactgataa acaggggttc aattatgcca cggtgacacc
    27181 cacggaaagt atagtgggaa caacaggtgg ctatactgtg catggtgtgg ttgcagcaga
    27241 cagattcccg ctggcaagtg gtgaatgttt cactttttcg tgccgggtta aaggcgctaa
    27301 agcacgatgc aggttaagag tttcagttat tattggtgga acagatacct tctctgctga
    27361 ctcttatctt gatctggata cccggatcgc aacagtaagc ggtaatacat cccttataac
    27421 agccaaagct gaacaacagg gcgagtggac ctactatgag gccacttata cagctaatac
    27481 ggacattgat accgttaact gtgcttttta tatgacaaat aaaataagta atgagacatt
    27541 ctatgatgac tcaacattaa ccatgacgac gccgcaaatt gaactgggca atacggcatc
    27601 gtcatttatt gtaactacaa tgccaacaac acgcgcaagt gatgtagtta ctatcccatc
    27661 gcagaataac ctgtcaacac ggccttttac ggtattgtgc gaagtaagca ggaactggag
    27721 tacaccgccc aatgttgcgc caaggatatt tgatgttgga gggcacagta ttgatgataa
    27781 ttatttatcg ctggggtttg ttgcaacagg aaagataagc gccaacgtag gaatggttca
    27841 gccacaaatt gcctcagatg gagaaaggtt cattgtgggt gtgagagcta aatctgattt
    27901 atcagtaaat gcaatatgca atggtaatta tacaacaaac cttaatggta aaatatttgg
    27961 agttacagca acatcgtacc ggtttggtgg gcagaccgca gcaggaacgc gtcatttgtt
    28021 tggacacatc agaaatttca gagtctggtt taaagaatta aatgacaggc aaatcaagga
    28081 ggcagtatga aagatttaac tttgaaattt cctggtaaca gagagtttaa atccttcctg
    28141 tcatctcttg actgggagga ggatgaagac ctccagaata aactgttagt cgatgaaatt
    28201 ggtttcacct acacagaaac aggggtaaca gaagagggag aacctgtctg tgtccggaat
    28261 gacggttatt ttgtcaacat tcgcattctt gatgacttgt ttgatgtttc tgtattctct
    28321 gattatgtcg tggagctgga aacaccgctt cgggaatgga gctgaaagga ggaaataatg
    28381 gatataagcc ccttacttca tgcactttgt gctgtggctg cgcagatact ggttggtctt
    28441 tttaccggaa actgggctta cggagcgata gccggttgta cgttcttcat tgcgcgtgaa
    28501 catacccagg cagaatatcg ctggattgaa atgttcgggc atggcaagcg aatgaatatg
    28561 ccgtggtggg gcggttttga tccgcgagct tgggatgtgg caagcctgat ggattttgct
    28621 gtgccggtgg tggcgtgtct gctggtctgg ctgttggtta atcgtgggtg aaaaaaggtg
    28681 agctgtatat gcaacggagg aagaaacccc gttgctggaa gccaggaaaa agtatcgggt
    28741 gttgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggagtttc
    28801 cctttttgcg tgcgttgatg ctggtaacgg caaacgtcat gatgaacaaa accgcgaata
    28861 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacaaccag acaccagtca
    28921 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
    28981 ggagtaccgc aatggcgcca ggcgcaatga gcagagaaat ggagagaatg atacctaccg
    29041 ctttcagcgt cgccacaatg gtcagagaga ccatgcacag cagcccataa tgcagccatc
    29101 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
    29161 gccattttac gctaataaca agtgtaacca gcccggcaat aatcatcgtc tggattatat
    29221 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
    29281 tgactgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca
    29341 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcacagaata
    29401 ctccggcgac gaatgcccct gtagccaaag gtaaccccag aatccaggca agcacgatgc
    29461 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
    29521 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
    29581 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcctt
    29641 atgcgcacgc cgacgatttg ccaaaagccc atgggtgggc gcaaagacaa atgctaaaag
    29701 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
    29761 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatca tgagcaggcg
    29821 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
    29881 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
    29941 gaggagtttt aatctgcccg gatgcaatcc aatagcacgg gcgtgattct cgtcgaaaaa
    30001 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
    30061 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
    30121 aatgttcaca gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
    30181 gaagatcaga ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
    30241 gctgcccgca gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
    30301 atatgccccc gcaacacccg ggacaataga gtgcgacaac gcatcgccaa tcagcgacca
    30361 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca tcgccgagac
    30421 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatctttct
    30481 tccctctgca ccgctgacgg tcgatgagcc acaaaagggc gttcatcgtc agtaataatg
    30541 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctctagg
    30601 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
    30661 accgtataat cgcaaaatgt cgtgacggat ccgagattgt gtgttgagac aagcatcgtt
    30721 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tggcttcggt tttcacatca
    30781 acgccagtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat cgctctcgcc
    30841 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgtcgatg gcgaaaattc
    30901 accatatcga cacgttccag cgcatcggtg acaatttgac gatccctctt tttggcgata
    30961 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
    31021 aatgaccagt caacatcttc tgactgaggg acgtaggcaa ccaggttttt ctgtaacgcc
    31081 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
    31141 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
    31201 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
    31261 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccagctt
    31321 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtcggtaccg
    31381 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
    31441 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
    31501 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
    31561 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
    31621 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
    31681 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcattg cgttggtagg
    31741 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
    31801 gagcattatc tggcgacatc catgcatggg ggttaggttt gccctcatag ggtccttcgg
    31861 tgatccctac aggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
    31921 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
    31981 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
    32041 tggttatgga tgagacttct gcagcatctc ccgccacgtt tttggccata tccgcgatga
    32101 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
    32161 aggtgagtgc gagccccccc aagagcatgg ttactttttt tatcgagtgc ataatttagt
    32221 acctatagtg agcaaaatta gaacaaatat agcaacggct atgttttata gcacagccta
    32281 tacaaaatga aaatgattat cattagcagc tttgaggccc aaaacaggga ttaggttgtt
    32341 tcctgctggc gacgcgttat tgaggaaaaa agtaaggcag ctagtaggct ttgaaataac
    32401 gttttgcggt tggctgatgc aatttcgaga gtgcatgtat tgaatgattt ctagccgtta
    32461 ctgattttcg tattttttgt atgggaggat ttgcacctcc ccccaccgag cctccatgac
    32521 tttactccac tgtctctagg gctgctgtgt gccaggagcg gaagcagggg ggccagagac
    32581 tccttaaaag ttgatagctc gttttgcagg gagacttaca cggtaagcgt tattccgaag
    32641 agcttaaaat tgaagcagtc aaacgggttg ttgagcgtgt ttatgcgctg ccatctgatt
    32701 aatcgacgga agcagttccg tttggtctat gatgcagttg tccggcaggc gctctgtgac
    32761 gcaaaagaac gataggagta gcattgaagt gaacgaggtt atgcttgtct tatattatcc
    32821 tctattttaa aaacatcagc tggttgtgta agagttttta ttgttttatc ttgttgactt
    32881 tgc
//
LOCUS       DACADA010000037        32295 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.037, whole genome shotgun sequence.
ACCESSION   DACADA010000037 DACADA010000000
VERSION     DACADA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.037"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(556..645)
                     /locus_tag="ISB17_001739"
     tRNA            complement(556..645)
                     /locus_tag="ISB17_001739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Ser,seq:cga)
     gene            739..1536
                     /gene="mtfA"
                     /locus_tag="ISB17_001740"
     CDS             739..1536
                     /gene="mtfA"
                     /locus_tag="ISB17_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAP1979682.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1637..1712
                     /locus_tag="ISB17_001741"
     tRNA            1637..1712
                     /locus_tag="ISB17_001741"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1670..1672,aa:Asn,seq:gtt)
     gene            1874..3136
                     /locus_tag="ISB17_001742"
     CDS             1874..3136
                     /locus_tag="ISB17_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1979683.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(3330..4634)
                     /gene="ybtS"
                     /locus_tag="ISB17_001743"
     CDS             complement(3330..4634)
                     /gene="ybtS"
                     /locus_tag="ISB17_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HAP1979684.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4662..5942)
                     /gene="ybtX"
                     /locus_tag="ISB17_001744"
     CDS             complement(4662..5942)
                     /gene="ybtX"
                     /locus_tag="ISB17_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HAP1979685.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5935..7737)
                     /gene="ybtQ"
                     /locus_tag="ISB17_001745"
     CDS             complement(5935..7737)
                     /gene="ybtQ"
                     /locus_tag="ISB17_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HAP1979686.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWGHE"
     gene            complement(7724..9436)
                     /gene="ybtP"
                     /locus_tag="ISB17_001746"
     CDS             complement(7724..9436)
                     /gene="ybtP"
                     /locus_tag="ISB17_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HAP1979687.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVVRLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            9693..10652
                     /gene="ybtA"
                     /locus_tag="ISB17_001747"
     CDS             9693..10652
                     /gene="ybtA"
                     /locus_tag="ISB17_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HAP1979688.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFTPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10843..16950
                     /gene="irp2"
                     /locus_tag="ISB17_001748"
     CDS             10843..16950
                     /gene="irp2"
                     /locus_tag="ISB17_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HAP1979689.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNCYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            17038..26529
                     /gene="irp1"
                     /locus_tag="ISB17_001749"
     CDS             17038..26529
                     /gene="irp1"
                     /locus_tag="ISB17_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HAP1979690.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26526..27626
                     /gene="ybtU"
                     /locus_tag="ISB17_001750"
     CDS             26526..27626
                     /gene="ybtU"
                     /locus_tag="ISB17_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HAP1979691.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27689..28426
                     /gene="ybtT"
                     /locus_tag="ISB17_001751"
     CDS             27689..28426
                     /gene="ybtT"
                     /locus_tag="ISB17_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HAP1979692.1"
                     /translation="MCPFAGGSSSAFRHWQAEQLADCALSLVTWPGRDRLRHLEPLRN
                     ITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFETCRLLEQRGLAPQGLIISGCHA
                     PHLHSERQLSHRDDADFIAELIDIGGCSPELRENQELMSLFLPLLRADFYATESYHYD
                     SPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLSHVTGPVVIDGDHFYPIQQARSF
                     FTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28430..30007
                     /gene="ybtE"
                     /locus_tag="ISB17_001752"
     CDS             28430..30007
                     /gene="ybtE"
                     /locus_tag="ISB17_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HAP1979693.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENLAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30138..32159
                     /gene="fyuA"
                     /locus_tag="ISB17_001753"
     CDS             30138..32159
                     /gene="fyuA"
                     /locus_tag="ISB17_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HAP1979694.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6972 a         9680 c         8923 g         6720 t
ORIGIN      
        1 ttttttatta tgtctgtaga aaaaaactga gaaaaaggcg aacaataaat acacaactca
       61 gccatacaga ctggcagcgc aaatgtctcc aggagcaagt catctgtcat ggtaaagcta
      121 accagaaata taggttaatt ttggtgttta aacctttttt acttgttagt atgatacaaa
      181 ggctttcaaa aaagctggga aattcaacaa ctcttggaaa ttatggactt tgtcccgcgg
      241 ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc tctacgcaac
      301 ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa aagaataatg
      361 gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct tcctgtcaga
      421 cccatagcga atcaagtgct gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac
      481 ccttcaagaa tatcctacga ttgttctgtt taggaaaagc aaggcgggaa gtcgggagat
      541 aagtcattga taaagtggcg gagagagggg gatttgaacc cccggtagag ttgcccctac
      601 tccggttttc gagaccggtc cgttcagccg ctccggcatc tctccgttca gatggttgcc
      661 atgatgccag gaaatttggc attttaacag tccctgtccg tgcaattttg ttcaagtgac
      721 gagtttgcga gcaaaacgat gattaagtgg ccctggaaag tacaagaatc agcacatcaa
      781 actgcccttc cctggcagga agcactatcg atcccccttt taacgtgtct gacagaacag
      841 gaacaaagca aattggtcgc tcttgccgaa cgttttttac agcaaaaacg gcttgttcct
      901 ttacagggct ttgagctgaa ttcattaaga agctgccgga tagcacttct attttgcctg
      961 cccgttctgg agttaggact ggaatggctg gatggttttc atgaaatctt aatttatcct
     1021 gcgccatttg tggtcgatga tgaatgggaa gacgatatcg gtctggtgca taaccaacgt
     1081 attgttcagt caggtcagag ctggcagcaa gggcctatcg ttttgaactg gttggatata
     1141 caagattctt ttgatgcttc tggttttaac ctgattattc atgaagtcgc tcataagctg
     1201 gacacccgta acggcgatcg cgccagcgga gttcccttta tttcgttgcg tgaggttgct
     1261 ggctgggaac acgatcttca tgctgcaatg aacaacattc aggaagaaat cgaattagtt
     1321 ggtgagaatg cggcgagcat tgatgcttat gctgccagtg atcctgctga atgttttgcc
     1381 gtactttctg aatatttctt tagcgcccca gaactttttg ctcctcgttt cccttcattg
     1441 tggcaacgtt tctgtcaatt ttatcaacaa gatcctttgc agagactgca tcacgctaat
     1501 gatacagact cgttttcggc gacgaatgtt cattaattaa caactttgca gattaattaa
     1561 ccaattgaaa tggcttatga aatttagtgt tgacagacaa ggtaccgcta agtaatatgc
     1621 gccccgttct cacgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta
     1681 tgtcactggt tcgagtccag tcagaggagc caattttctg ttttcatgca tccttgcgaa
     1741 tctttatctg atgttgattc aacaggttag tgtgaaaact cttctcagtg cattttgatt
     1801 ttaccctacg catcgggaaa aattggtggt caaatctggg gtcaggttag ttcgataatg
     1861 gagtgacccc catatgtccc ttaccagcgc aaaaatccgc accctcaagc cttctgataa
     1921 accctttaaa gtctccgatt ctcacggtct gtatctgcgg gtcaagccgg gtggctcccg
     1981 ccactggtat ctcaaatacc gtattagcgg taaagaatcc cgcattgcgc tgggtaccta
     2041 tccagccatc tccctgtctg atgcgcgaca gcaacgtgaa ggtatccgta aaatgctggc
     2101 gctgaatatc aacccggtac agcagcgggc tgctgtacgt ggctcacgaa caccggagaa
     2161 agtttttaaa aacgtggcgc tggcgtggca taaaagtaac aggaaatggt cgcagaacac
     2221 cgccgaccgt ctgcttgcca gcctgaacaa tcacatcttt ccggtcatcg ggaacctacc
     2281 tgtatcagaa cttaaacccc gtcatttcat tgacctgctg aaagggatcg aggaaaaagg
     2341 tctgctggag gttgcgtccc gcacacggca gcacctgagt aacataatgc gccatgcggt
     2401 ccatcaggag ttaatcgata cgaaccctgc agcaaacctt ggcggcgtga ccacacctcc
     2461 tgtcagacgg cactatcctg ccctgccgct ggagcggctg cctgaactgc ttgaacgtat
     2521 tggggcatat catcagggcc gtgaactgac ccggcatgcc gttctgctga tgctgcatgt
     2581 gttcattcgc tccagtgaac tgcgtttcgc ccgctggtca gagattgatt tcacaaaccg
     2641 agtctggacg atacccgcga cgcgagaacc cattattggc gtgcgttatt ccggccgcgg
     2701 ggcaaaaatg cgaatgccgc atatcgtccc cctctcagaa cagtccatcg ccattctgaa
     2761 acagattaag gatatcaccg gtaataatga actgatcttc cccggcgacc ataacccgta
     2821 taagccaatg tgtgaaaaca cggtcaataa ggcactgcgg gtgatgggtt acgacacgaa
     2881 aaaggatatc tgcggtcacg gcttccgggc aatggcatgc agtgcgctga tggaatcggg
     2941 tttatgggca aaggacgcag tagaacgcca gatgagtcat caggagcgca ataccgtgcg
     3001 catggcttat attcataagg cagagcacct ggaagcccgc aaagcgatga tgcagtggtg
     3061 gtcggattat ctggaagcat gccgagaatc ttatgcaccg ccttatacaa ttggtaaaaa
     3121 taagtttatc ccatagtata ctaacgaatc ccacactctg gcaaattacc accacctcca
     3181 caagaggtgg ttctatatag acttttatca acttcatgac gaaaaacacc tattttatcc
     3241 caaaagaaga caatagagtc acagaggaat caaagagcga ctcaattgtc tttggacagt
     3301 ctggttgtga ggttagaaaa cagttactcc tacaccatta aatagggcgc aatgctcgct
     3361 aatttctccc gggtttctgt cagttcgcgt tccggcgtag attgcgcgat gattcccgcc
     3421 cccgcctgta tccagcagcg ctggctatcc tgaaatacgg aacgcagaac tagcgccgca
     3481 tcaaagcgcg tatcgtccag cagcaggatt gcgccggaat aaagctctcg cggcgtcttc
     3541 tcaatttgca taatcgcgtt cagagcagca tttttaggga tacctgaggc ggtaatcgac
     3601 ggaaacagca cggtaaacgc atcccaggca tccttgtttt ccgcaagctg accgctcacg
     3661 ccagacccca gatgctgaac gctgccgcgc tggcgaaccg acattaaatc ttcaaccacc
     3721 acgctgcccg gctggcaaac ggcctccagt tcagcaatag cttctttgac agaaaggata
     3781 tgctcaagca cctctttact gtcgtgcagc agttctgcct ctttcgcctt attatgctcc
     3841 gggtttccca tgcgatcgcg ggtgcccgca agcggttcag tgaccacttt attgcccgtc
     3901 actgacatca ccagttctgg gctaaagccc agcgcttcgc gtccttcctg acggaacata
     3961 aacgagcgca caggcgtgtt tgcctgccgc ccgtataaca gcgtggcggg catatcaatc
     4021 cgcgatggca ggggaatggc gcgcgagaca atcactttga catactcgcc acggcggatc
     4081 tccgcaacgg cgcgcgcaac ctgttgttta tatgcctcac cattgagggc ggtatccacc
     4141 gccagtggtg cgttctgcgt cgttgtaccg gcctctttta cccactcgca caaacgtcgg
     4201 cacccgtcgg cggagtccgc ataaacggtg acatttccct tttcaaaaat aagttcttca
     4261 cggggaacgg ttaacgtcag cagcggccac tccccggcgt taaaggcaat cccccgcgca
     4321 tgcgccgcaa agttgaaccc aacctgtcca tatacccgac gtcctttcac ctgaggatgc
     4381 gccataaaac gacgcgcgca gtcggcaatg gaatccaccg cccatttttg ctccccggca
     4441 tcatcaataa atgtcgcctg cgttccgtcg gcattaatgt gcagccgtgc ctggcaccct
     4501 ttgcctaaat accaacaggg ttgacgctca tagacataac actcttcttc tgcgaactgg
     4561 cgtaaaacgc cagaaatcgt cggtagccat tgttcctctg gtaacgccag atgtagaaat
     4621 tcactgattt tcatccctta cctctctgtg ttattcgcgg atcaatcaat tgcgcttgtc
     4681 atcagttctc ttgccgaatg cagccggata taagcaaccc acgccgccag caacgtgaat
     4741 gccccggcaa acaggaagca ggccgcatag ccaaaatgtt gagcaacaac gccaccgata
     4801 acgcctgcca gaatggcgat tgccgcatca gtacattgaa agagggtgaa gtcgacaccg
     4861 gcctgcaaag gcgatgaaag cgacatcagc gtggcgtaaa gacagaccag cgcgcaggcc
     4921 agcgaaatgg actggacaat gaccagacac tggagaatct gcagcagcag atgacccggc
     4981 gccatcatga gcgtcatcac caccgccagc agcgcgatcc cctggacgcc ataagccgtc
     5041 aacagcgctc tgccgggtga ggtgtatttc atcagcaatc cgccggccag cgttcctgct
     5101 atgcccgctg caatattgcc gccgctgaac agcgcgccca atgcggacat gctcaaccca
     5161 tgatccaaca acagcggtgc cagaagaggc agcacaaacc gcatgcctga attcagcatc
     5221 aataccagca gtaagcccag gcgcgcctgc ttcctcctta gcgcataacc taacgccgga
     5281 acatgcggga tagtcgctgt tcgcgtcggc tccgtaatgc gccacagcgg gagtgacagc
     5341 gccataatca gcaccgccat catcagcatg gcgacaggcc agccggatgc cgccgacagc
     5401 atcatgaaca ccccgccgcc gcacatcatt cccagatagc tgccgccgac ctgcacgctg
     5461 tttccccagc cgtaacccgt gcgggtcagt tgatcgacac aaaagccgtc gctggcgata
     5521 tcaatggtgg aggcgaccgt gcctgccacc ataaacagaa caaataacgc gacgatttgc
     5581 gtatcgggga atcccccttc tcgcccaaac cagccaatcg ctgcggcaat agtcaggatc
     5641 gccgctagcg cgacctgacc acgcaggatt aacatgcggg aacggcgttc ctggctaccg
     5701 ggcggaagcc gccagcgctc gatccacggc gcccaaataa acttcagcgc ccagggcagc
     5761 ataaacaggg ttgtcgcacc ggcaagcgcc agcgaaccgc cagcagcgcg caccagcgcg
     5821 ggtaaggact gcatagacag cgccgatacc agactctgcg tcacatagac gccggcaata
     5881 gcaaaaatca cccgcccggt cgtcaacgtc agcggtttca cattcgactg aacatcactc
     5941 atgcccccac tctccagacg cggaaacccc gtcgtcgcgc cacacgcgcg cggccatttg
     6001 cgcctgccac agcgcctgat aacgtccatg atgtgagagc aattgcgcat gagtaccctg
     6061 ctcaaccacc tgtccctctt ccatcaccag aatgtttccg gccccggcga tggtggataa
     6121 acggtgagcg atgataatca ccgtccggtt gtaaaccagg ttatcgatcg ctttttgcac
     6181 cgccagctcg ctttcaatat ccagcgcggc agtcggttca tcgagaataa cgaccggcgc
     6241 gtttttcaat aacgctctgg caatggaaat ccgctggcgc tcgccgcccg atagctggcc
     6301 gcccatctct cccattggcg tcagccagcc ttgcggaaga cgggaaataa actcaaggca
     6361 ctgcgccgcg cgggcggcct cttctacctc ctgccgcgtc gcctgtgggc gcgcgatacg
     6421 gatattcgcc agcagcgtgt catcaaacag ccagacatcc tggaaaacaa cagaaatcag
     6481 gctattgagc tgttccggcg tcaggcggcg aatatcgacg ccgccaatag aaatctgccc
     6541 ttgctgcgga tcggcatagc gcattaacag tttggtgacg gtagttttgc ctgcgccgga
     6601 ggcacccacc agcgcgctca tactggctgc cgggaacgtc aaagaaacat gattaagcgc
     6661 gtggccgtca ccttcttcgt agcgatagct gacgttgtca aagcggatat cgtaacgttc
     6721 cggcatctcg ctttgctctg cgacgggaag cggtgctatc gccataaact gctcaatcct
     6781 ttgcagggcg ctggcgatca gttccacaac cgaggtgtag ctgataaaca tcgccatcgg
     6841 ttctgcgaag cgcataatca tcgcgacggc ggcaatcaaa aaggcgaggt tcagagtgcc
     6901 cgtcactacc cagacgatcc cggataacac caccacctgt aggcccaact ccacgacgct
     6961 ggcgatcagc atcgtcgcgc cagcgccctg acggtgagtg cgggtctgta agttttccag
     7021 cgcattgaaa tgcgccagca gcgcccggct tttatcggca tcgctgccgc aggtgcgcaa
     7081 taccatcatc ccctgagcaa attcaacgat atcgccgctc agacgctggt gcgcttcccc
     7141 cagcgtctgc atttgtcgcc gcatcgccgg gcggcgccag taataaaacg gcaccagcag
     7201 agggaagatc agcaacatca ccagccccag ccgccagtct atccacaatg tcgccagcga
     7261 cgccgtcagc ggcgtgacaa tggtgagcag caaaatattg gctatcgcaa taacataatt
     7321 gaggttttca tccacgctgc ccagcagcaa ggcgttcatt tcacccgccc tgccgcgctg
     7381 gagcttctcc agcggcacgc ggcgtaactg ctcgccaagt cgcaggcgca actcatgggt
     7441 agcctgcgcc agatgaccac ggtattcaaa gcccaggcca taccagcgta gcaccagcgt
     7501 cacaattgcg gcgacgctga aggccatagc ccagttaaga agttgcggcg cgtctccccg
     7561 taacagcgca tcaatgatcg gataaagaca ggcaaaggcg atcccctgca tgaatgccgc
     7621 cagcaacagc gccagcatac tgcgtcgcag catcctggcc tgactgccaa cgctggagat
     7681 aagctggcgc cagatgacgc gccaggccag gttatccgca ggattattgt ctttcatttt
     7741 ccatcacctc ttcctgtgtt ccacccggca cccaatgctg cgcctgctga tagtgttgcc
     7801 agagccgttg atacaggccg ccctgcgcca acagttgcgt atggttcccc atttccctga
     7861 gctgtccgtc ggaaaacagc aatatcacat cggcctgagt caccattgag agacgatgcg
     7921 cgaccatgat gaccgtccgg ccacgcatgg ccgccgcgag cgccttgata agcgccgctt
     7981 cgttttccgg gtcagcaaac gccgtcgcct catcgagcac caggatgggg cgatcctgca
     8041 aaagcgcccg ggcgatagta atgcgctgcc gctggccgcc ggagagaaat accccacgtt
     8101 ccccgactcg agtgttgtaa ccttctggca gagcgctaat aaaatcatgg gcctgcgcca
     8161 ctctggccgc cgctataacc gcctccagcg gcgtatccgg cgcgcccaga cgaatgttat
     8221 tggctatcgt gtcggcaaaa aggaagttgt cctgaaacac aaacgagagt tgcttcatca
     8281 gggtgtccgt ctgcatatca cgcagatcca cgccgccaat acggatatgg cctttgtccg
     8341 ggtcggcgta acgcagcagc aagcgcacca cggtgctttt tccggcgccg cttggcccga
     8401 ccagcgccac aatttgcccg gcaggcacat gaaagctcac ctcctgcaac gcggcgccag
     8461 tacgcgcttg cggataatga aagctcacct gctcaaaggt aatgctcgcc tcttgcggct
     8521 gctggtcaga ctgcggcagc gataactccg gcatcgcgag aacctgataa atacgctgaa
     8581 tgcttaaacg cgtttgcgcg accaggttat tgagcatcat cattggcatt acggcttcgg
     8641 ccattccgct gcccagcagt aaaacggcca cccacgcgat aaaatcgaaa ctgccatagt
     8701 gcaacaggcc gtatcccgac cagatcagga caaacagggt cgggagagga ttcagaatcg
     8761 agaaggaaaa acgcgctgaa aaaccggctt tgcgatacca ggttttgagc acatcgaccc
     8821 actcttcaag ggcgcgttga tagcgcaaaa aactggtgct gccgctatca aacgttctca
     8881 ccacgggcat ggcctgcacg aactcaataa ccgccgcgct aacctgctcg cgcgccttat
     8941 gataacgctg agccatatct tccgaacgct ggcgggcgag cacgagaaca accgatccaa
     9001 acgccagtac ccccagcgtc gcgattgcca gccgccagtc cagccagaac aagataacga
     9061 ttgtcgccag cggcatgatg atcgcgcggg catagagcgg cgtgctgtcg gccacaaaaa
     9121 tatgtaacga cttcacgtca tccagcatca ctttcgccac tgacccgctg cccatttgct
     9181 gtaacacgcc aggcggaagc tgcaatgctt tacgggctaa cctgctgcgc agaatgttct
     9241 ccagatgaaa agccgcgtaa tgagaggtgt tgaacgcctg taagcgcaga acaaaagtta
     9301 agaccacgca gccgatcacg ccgcccagcg gccaggccgg aatagcgtca ggcgtcgcgc
     9361 gaatgtcacg caggctccac gccagaaaaa ggagcgcccc caggctggtt aatgccgcca
     9421 gccccgccag cgccatagct aaaataacct tcctgcgaac cggtgtaatc acctgccaaa
     9481 gaggaaatcg ggacagcgat tcagtattgg atgattgaga tgacatggga gtaactgaat
     9541 ttcctgatga atttttacgg acgccaaaaa cgacaattca gggtggcgtt ctgagaatta
     9601 atgtgaataa taaccattat caataactca tctaccccat tcggttctat ttatacccgc
     9661 attggtctaa gccacaggga gataaccagg tcatgacgga gtcaccgcaa acgcaatctg
     9721 aaatctctat tcaccagttg gtggtcggga aacctgcaaa cgatggcaat attcctgctc
     9781 agtgtgaact gctgcgctgt tcgttgcagg aaggtatgga tattttgctc tggaggggcc
     9841 attttgcgcg gcctgagacg ttgcagctac acgacgatct gggcaggatt aacttctcgt
     9901 gcatcctgga aggcacatcg cgctttgcga ttcagggact ccggcgtcac accgactggg
     9961 agcttgcgcg caatcgccat tacattaccc atacgccaga ctgtcgcggc agcgcctcct
    10021 attgtggccg attcgagagc attaccctct ccttcagccc tgaaaccctc gcgctgtggg
    10081 tgccggatat tagcgcggtc attaagaata agatcgactc ccactgctgt tgccagcagc
    10141 accgttgtaa tgctgaaacc cacttaacgg ctcaggcctt gcgtcacgcg ctgatgcgca
    10201 tgcacagcgg cttcagtcat gaacaaaagc cgtcgacgct atggttgcag gggcaaagtc
    10261 tggtcatgct cagtctggtg ctggatgaac atcgcgaaga cgccagttgc ctctcctgcc
    10321 acttcacccc gatggaacgc cagaaactgc tgcgggcgaa ggatctgctg ctggctgatc
    10381 tgacgcaagc gccgggcgtc gcggagctgg ccagagagtc cggtctgagc gttctcaaaa
    10441 ttaagcgagg cttccgcgtt ctgttcaaca acagcgtcta tggcctgttt caggctgaac
    10501 gaatgcagga agccagaaga cgcctggcca acggcaatac ctccgtcatg acggtggcgg
    10561 ccgatctggg ctatgccaac gccagccatt tctctgccgc cttccagaaa cagtttggcg
    10621 taactccttc gacctttaaa cgcgggatgt aaccggggtc gcgcccccct aaactcctcc
    10681 ctgacagagg cccggatacc ccatcagggc ctctgttctt tccgcctgtt accaacccga
    10741 aacgggcata aataaacccg tttcgggtag catccaattg ttaataatta ttattctcat
    10801 atgagtaatg cttttcggta agacgtgcca tcaggaggaa gaatgatttc tggcgcacca
    10861 tctcaggatt cgctgttacc ggacaaccgc cacgcggctg attaccaaca attacgcgag
    10921 cggctcatac aggaactgaa tttaacgccg cagcagttac atgaagagag caacctgatc
    10981 caggccggcc tggattccat aagattgatg agatggttac actggtttcg taaaaattgc
    11041 taccgcctta cccttcgcga gctgtatgcc gcccccacgc tggcggcatg gaaccagtta
    11101 atgctcagcc ggtcgccgga gaacgcggaa gaagaaacgc cgcccgacga atcatcctgg
    11161 ccgaacatga ccgaaagtac ccccttccca ttgacgccag tacagcacgc ctacctgacg
    11221 ggccgcatgc cggggcagac gcttggcggc gtgggttgcc acctgtatca ggagtttgaa
    11281 ggccattgtc tgacggcgtc gcagctggag caggccatca cgaccttgct gcaacgccac
    11341 ccaatgctgc atatcgcctt tcgccccgac gggcagcagg tctggctacc gcaaccttac
    11401 tggaacggcg tcaccgttca tgatttacgc cataacgacg ctgaaagccg ccaggcctat
    11461 ctggacgcac tgcgccagcg cctgagccac cgtcttttac gcgtggaaat cggcgaaacg
    11521 tttgattttc agctgacgct cttgccggac aatcgccacc gcctccatgt caatattgac
    11581 ctgctgatta tggatgcctc cagctttacg cttttcttcg atgagcttaa cgccctgctg
    11641 gccggagaat cgctgccggc tatcgacacc cgctatgatt tccgctcgta tttgctgcac
    11701 cagcagaaga tcaatcaacc actgagagac gacgcgcgcg cttactggct ggcgaaagca
    11761 tcgacgcttc cccccgcgcc cgtcttgccg ctggcctgcg aacccgccac gctacgtgaa
    11821 gtccgtaata cccgacgccg catgattgtc ccggcaacac gctggcacgc ctttagcaac
    11881 cgggccggcg agtatggcgt gacgccgaca atggcactgg cgacctgttt ttctgccgtg
    11941 ctggctcgct ggggcggcct gacgcgtctg ctgcttaaca tcaccttatt cgaccgccag
    12001 ccgctgcacc cggcggttgg cgcgatgctt gccgacttca ccaatattct tctgctggat
    12061 accgcctgcg atggcgatac cgtcagcaac ctggcgcgta aaaaccagct cacgtttacg
    12121 gaggactggg agcatcgcca ctggtccggc gtcgaattac tccgtgaact caaacgccag
    12181 cagcgctacc cccacggcgc cccggtggta tttaccagca atctggggcg ttccctctac
    12241 agcagccgcg cagaatcgcc gttgggcgag ccggaatggg gcatctcgca aacgccgcag
    12301 gtctggatag atcatctggc gttcgagcat cacggcgagg tctggctaca atgggacagc
    12361 aacgacgcgc tgttccctcc ggcgttagtc gaaacattgt tcgataccta ctgccagttg
    12421 attaaccaac tctgcgatga cgaaagcgcc tggcaaaagc cgttcgcaga tatgatgccc
    12481 gccagccagc gcgcgatacg cgaacgggtc aacgccaccg gcgcccccat tcccgaaggc
    12541 ttgctgcatg aaggcatttt ccgtatcgct ctgcaacagc cgcaggcgct ggcggtaacg
    12601 gacatgcgtt atcagtggaa ttatcatgag ctgacagact atgcccgccg ttgcgcgggc
    12661 aggttaatcg agtgcggggt tcagcccggc gataatgtgg ctatcacgat gtcgaaaggt
    12721 gcaggacaac ttgttgcggt tctggccgtc ctgctggctg gggcggttta cgttccggtt
    12781 tcgctggatc agcctgccgc acggcgcgag aaaatctacg ctgacgccag cgtccggctg
    12841 gtgctcattt gtcagcacga cgccagcgcc gggtcagacg atattcccgt ccttgcctgg
    12901 cagcaggcca ttgaggcgga gccgatcgcc aacccggtag tacgcgcccc cacgcaaccg
    12961 gcctacatta tctacacctc cggctctacc ggtacgccga aaggggtagt catttctcac
    13021 cggggagcgc ttaacacctg ttgcgatatc aatacccgct atcaggttgg cccgcatgac
    13081 agggtgctgg ccctctccgc cctacatttt gatttatcgg tttacgacat ttttggcgta
    13141 ctgcgcgcgg gcggcgcgct ggtgatggtg atggaaaatc aacggcgcga tcctcacgca
    13201 tggtgtgagc tgatccagcg ccatcaggtc acgctctgga acagcgtccc ggcgctgttc
    13261 gatatgctgc tgacctggtg tgaaggtttc gccgacgcca cgccggaaaa cctgcgcgca
    13321 gtgatgcttt ccggcgactg gatcgggctt gacctccccg cccgttatcg ggccttccgg
    13381 ccacaaggac aatttatcgc gatgggcggt gccaccgagg cgtctatctg gtctaacgcc
    13441 tgcgaaattc acgacgtccc cgcccactgg cgctccatcc cttacggttt tccgctaacc
    13501 aaccaacgct accgggtggt ggatgaacag ggccgggact gccctgactg ggtgccgggt
    13561 gaattatgga ttggcggcat tggggtcgcg gaaggctatt tcaacgatcc cctgcgtagc
    13621 gagcagcaat ttttgacgct cccggacgag cgctggtatc gcaccggcga tctcggctgc
    13681 tactggccag atggcacaat cgagttcctc ggtcgtcgcg acaagcaggt caaagtcgga
    13741 ggatatcgca tcgagctggg cgaaatcgaa agcgcgctca gccagctggc gggggtgaaa
    13801 caagcaaccg ttctggcgat cggcgaaaaa gaaaaaacgc tggcggcata cgttgttcct
    13861 cagggcgagg ctttttgcgt taccgatcat cggaacccgg cactgccgca ggcgtggcac
    13921 acgcttgcgg gaacgttgcc ctgttgcgcc atctcgccag agatctccgc agaacaggta
    13981 gccgatttcc ttcagcatcg cctgctaaaa ctgaagccgg gtcacaccgc tggcgccgat
    14041 cctctccccc tgatgaactc actcgctatc cagccgcgct ggcaggccgt ggtggaacgc
    14101 tggttagcat ttctggtgac acaacggcga ctgaagcccg ctgctgaagg ttatcaggtc
    14161 tgcgctggtg aagaacgcga ggatgagcac ccgcacttca gcggacatga tttaacgtta
    14221 tcgcaaattc ttcgcggtgc ccgtaacgaa ctgtcgttac tgaacgacgc gcagtggtcg
    14281 ccggaaagcc tggcctttaa ccatccggcc agcgccccgt atattcagga actggcgaca
    14341 atttgccaac agcttgcaca gcgcttacag cgcccggtgc gcctgcttga ggtgggaacc
    14401 cgcactggcc gcgccgcaga atcgctgtta gcacagctca acgccggaca gattgagtat
    14461 gtcgggcttg agcagagcca ggagatgctg ctgagcgccc ggcagaggct cgccccctgg
    14521 cctggcgccc gtctgtccct ctggaatgca gacacgctgg cggcgcacgc tcactcggcg
    14581 gacattatct ggcttaataa cgccctgcat cgtctgctgc cggaagatcc cgggctcctt
    14641 gcgacattac aacagcttgc cgttcccggc gcgctgctct acgtgatgga gtttcgccag
    14701 ttaacgccgt ccgccctact cagcacgctc ctgttaacca atgggcagcc ggaggccttg
    14761 ctgcataaca gcgccgactg ggcggcatta tttagcgcgg ccggcttcaa ctgtcagcat
    14821 ggcgatgagg tcgcggggtt acaacgcttc ctcgtacaat gtcctgacag gcaggtgcgc
    14881 cgcgatcccc gtcaacttca ggccgccctc gccgggcgtc tgccggggtg gatggtgccg
    14941 caacggatcg tcttcctcga cgccttaccg ctgacggcta acgggaaaat tgactaccag
    15001 gcgctgaagc gtcgtcatac ccctgaagcg gaaaacccgg ccgaagcgga tttaccccag
    15061 ggcgacattg aaaaacaggt tgccgccctc tggcagcaac tcttatcaac tggcaatgtc
    15121 accagagaaa ccgacttctt ccagcaaggc ggcgatagcc tgctggcgac ccgtctgacc
    15181 gggcaacttc atcaggcagg ttatgaagcg caattaagcg acctgtttaa tcatccccgg
    15241 ctggcggatt ttgccgccac gctgcggaaa accgacgtcc cggtcgaaca accattcgtc
    15301 cactcccctg aagatcgcta ccagcccttt gcgcttaccg acgtgcagca ggcttaccta
    15361 gtggggcgtc agccgggctt tgccctgggc ggcgtcggct cacatttctt tgttgaattt
    15421 gaaattgccg atctggatct cacccggttg gagacggtct ggaaccgatt aatcgcccgc
    15481 cacgatatgc tacgcgccgt cgtgcgtgat ggacagcaac aggtgctcga acagacgcct
    15541 cgctgggtaa tacccgcaca catcctccat acgcctgaag aggcgttgca ggtgcgcgaa
    15601 aaactggccc atcaggtact caaccccgaa gtctggccgg tattcgatct ccaggtcggt
    15661 tacgttgacg ggatgcccgc ccgcctgtgg ctgtgtctgg ataacctgtt acttgacggg
    15721 ctgagcatgc agattctgtt ggcggagctg gagcacggct accgctaccc gcaacagctg
    15781 cttccgccgc tgcccgtcac cttcagggat tatctgcaac aaccctcgct acagtcgccc
    15841 aatccagatt ctctggcatg gtggcaggcg cagcttgatg atattcctcc ggcgcctgcg
    15901 ttgccgctgc gctgcttgcc tcaggaggtt gaaacaccgc gcttcgcccg cctgaacggc
    15961 gcactggaca gcacgcgctg gcatcggctg aaaaaacggg cggctgacgc ccatctcacc
    16021 ccgtcggccg tactgttgtc ggtgtggtca acggttctct ctgcatggag tgcacagcct
    16081 gagttcacgc ttaaccttac gcttttcgac aggcgaccgc tgcacccgca aatcaaccag
    16141 attctgggcg atttcacctc gctgatgctg ctgagctggc atcccggcga aagctggctg
    16201 cacagcgcgc agtcactaca gcagcggctg agccagaacc tcaaccaccg cgatgtgtca
    16261 gccatccgcg tgatgcgtca actggcgcaa cggcaaaacg tgccagccgt tccgatgccc
    16321 gtcgtcttta ccagcgcgct gggctttgag caggataact tcctcgcccg gcgtaatctg
    16381 ctcaaaccgg tctggggcat ctcccagacg ccgcaggtct ggctcgatca ccagatttat
    16441 gaatccgaag gcgaactgcg ctttaactgg gattttgtcg ccgcgctgtt tcctgccggg
    16501 caggtggagc gccagtttga acagtattgc gcattgctaa accgaatggc cgaggatgaa
    16561 agcggctggc aactgccgct cgccgcgctg gtgcctcccg ttaaacacgc agggcaatgc
    16621 gcagagcgct caccgcgcgt atgccctgag cactctcagc cacacattgc ggcggacgag
    16681 agcaccgtca gcctgatttg cgacgccttc cgcgaggtgg ttggcgagtc tgtcacgccc
    16741 gcagaaaact tctttgaggc gggcgcaacg tcgctgaatc tggtgcaact gcacgttttg
    16801 ttacaacgtc acgaattttc caccctgacg ttgcttgacc tcttcaccca cccttctcct
    16861 gctgccctgg ccgattatct ggccggcgtc gccacggtgg agaaaacaaa acgacctcgc
    16921 cctgttcgcc gtcgtcagcg gcggatatag cgcgaagcaa actgattttc cccggaacgc
    16981 catcgcgaac gcatggcgtt ccattgactt tgtgaatctt aggaaacggg accgattatg
    17041 gataacttgc gcttctcttc tgcgccgaca gcagattcca ttgatgcatc gatcgctcaa
    17101 cactacccgg actgcgaacc tgtcgcggtt atcggctacg cctgccattt tcctgaatcg
    17161 ccggatggcg aaacgttctg gcaaaatctg ctggaaggtc gtgaatgcag ccgacgcttt
    17221 acgcgcgaag agcttctggc cgtcggtctg gatgccgcca tcattgacga tcctcattat
    17281 gtcaatatcg gtacggtgtt agacaacgcc gactgcttcg acgccaccct gtttggctat
    17341 tcgcgacagg aagcggagtc gatggacccg cagcagcgcc tgtttttgca ggcggtctgg
    17401 catgcgctgg aacatgccgg ttatgccccc ggcgccgtcc cccataagac cggcgttttc
    17461 gcctcttccc ggatgagtac ctaccccggt cgcgaagcat tgaacgtgac agaagtcgcg
    17521 caggtaaaag gtctgcaatc tctgatgggc aatgataaag actatattgc cacccgcgcc
    17581 gcgtacaaac tcaacctgca cggcccggcg ttatcggtac agaccgcctg ctccagctcg
    17641 ctggttgccg tgcatctggc ctgtgaaagc ctgcgcgcag gcgaatccga tatggcggtt
    17701 gccggcggcg tggcgctctc tttcccccag caggcaggct accgctacca gcccggaatg
    17761 attttctctc ctgatggtca ctgtcgtccc tttgacgcct cggctgaggg cacctgggcc
    17821 ggtaacggtc tcggctgcgt ggtgctgcgt cgcctgagag acgcgctgct gtcaggcgat
    17881 ccgattatct cggtgatcct ctccagcgcg gtcaacaacg acggcaacag aaaggtcggc
    17941 tataccgccc cttccgtcgc agggcaacaa gcagtcatcg aagaggcgtt aatgctggcg
    18001 gccatcgacg acaggcaggt aggttacatt gaaacccacg gcaccggcac accgctgggc
    18061 gacgcgattg aaattgaagc gttacgcaac gtctatgcgc ctcgcccgca ggatcagcgc
    18121 tgtgcgctcg gttccgtgaa aagtaacatg ggccatctgg ataccgcggc gggcattgcc
    18181 ggactgctga aaaccgttct ggcagtcagt cgcgggcaaa ttcctccctt actgaatttt
    18241 cacaccccca acccggcgct gaaacttgaa gagagcccct ttaccatacc ggtgtcggca
    18301 caggcatggc aggacgaaat gcgctatgcg ggcgtctcct cctttggtat tggcggcacc
    18361 aactgccata tgatcgtcgc ctcgctgccc gacgcgctca acgcgcgcct ccccaatacg
    18421 gatagcggca gaaaaagtac cgcgctgctg ctcagcgccg ccagcgacag cgcgttgcgg
    18481 cggctggcga cggattatgc cggggcgctg agagagaatg cggatgccag ctctctggcc
    18541 ttcacagccc tgcacgcgcg ccgtctcgat ctccccttcc gcctggcggc gccattaaac
    18601 cgtgaaaccg ccgaggcgct cagcgcctgg gccggtgaga aatcgggggc gctggtttac
    18661 agcggccacg gcgccagcgg caagcaggtg tggctgttta ccggccaggg ctcgcactgg
    18721 cgcactatgg gtcaaacgat gtaccagcac tcaacggcgt ttgccgacac gctggatcgc
    18781 tgtttttccg cctgtagcga aatgctcacg ccgtcactgc gcgaagcgat gtttaacccc
    18841 gattcggcgc agctggacaa tatggcctgg gcgcagccgg cgattgtcgc gtttgaaatc
    18901 gcgatggcgg cgcactggcg tgctgaagga ctgaagccag acttcgccat tgggcattcc
    18961 gtcggtgaat ttgccgctgc cgttgtctgc ggacactata cgattgaaca ggtcatgcca
    19021 ctggtttgtc ggcgcggcgc gctaatgcag cagtgcgcaa gcggcgcgat ggtggcggta
    19081 tttgcagacg aagacacgct gatgccgctg gctcgccagt ttgagctgga tctcgccgcc
    19141 aacaacggta cgcaacatac ggtattttcc gggccggaag cccgtctcgc ggtattttgc
    19201 gccacgctct cgcagcatga cattaactat cgtcgcctga gcgtaaccgg tgcggcgcac
    19261 tccgctttac tggagccgat actcgatcgg ttccaggacg cctgcgcggg actgcacgcg
    19321 gagccggggc aaataccgat tatttccacg ctcaccgccg acgtcattga tgagtcaacg
    19381 ctcaaccagg cggattactg gcgccgacac atgcgccagc cggtgcgttt tatccagagt
    19441 attcaggtgg cgcatcagct cggcgcccgc gtttttctgg agatggggcc cgatgcccag
    19501 ttggttgctt gcgggcagcg cgaataccgc gataacgcat actggatagc cagcgcccgg
    19561 cgtaacaaag aggcgagcga tgtcctcaat caggccctgc tccagcttta cgctgccggc
    19621 gtcgccctac cgtgggccga cctgctggcg ggcgatggac aacgtatcgc tgcgccatgt
    19681 tatccgtttg atactgaacg ttactggaaa gagcgcgtct ccccggcctg cgagcctgcc
    19741 gacgcagcgc tgtctgccgg gctggaggtg gcgagtcgcg ccgcgacagc gctcgatctc
    19801 cctcgcctgg atgcgcttaa acagtgcgcc acgcgactgc acgccatcta cgtcgatcaa
    19861 ctggtacaac gctgtaccgg cgatgccatt gagaacggcg tggacgccat gaccatcatg
    19921 cgccgtggac gtctgctgcc ccgctaccag cagctactcc agcgcctgct gaataactgc
    19981 gtggtcgacg gcgattaccg ctgcaccgac gggcgatacg tccgcgcccg ccccattgaa
    20041 catcaacagc gggaatcact gctgacggaa cttgccggtt attgtgaagg ttttcaggct
    20101 attcccgaca ccatcgcccg tgccggcgat cggttatatg aaatgatgag cggcgcggaa
    20161 gaaccggtgg cgattatctt cccgcaaagc gcctccgacg gcgtggaagt gctgtatcag
    20221 gaattcagct ttggccgcta tttcaaccaa atcgccgccg gggtattacg cggcattgtc
    20281 cagacgcgtc agccccgcca gccgttgcgt attcttgaag ttggcggcgg aaccggcggc
    20341 accaccgcgt ggctgctgcc ggaactcaac ggcgttccgg cactggagta ccatttcacc
    20401 gatatctcgg cgctgttcac ccgtcgcgcc cagcagaaat tcgccgacta tgattttgtg
    20461 aagtatagcg agctggatct cgaaaaagag gcgcagtctc agggtttcca ggcacagtct
    20521 tacgatctta tcgtggcagc gaacgtgatt cacgccaccc gccatattgg ccgcacgctc
    20581 gataatctgc gccccctgct caagccgggc gggcgcctgc tgatgcgcga aatcacccag
    20641 ccaatgcgtc tgtttgactt cgttttcggc ccgctggttc ttccgctaca ggatctcgac
    20701 gcccgcgaag gtgagttatt cctcaccacc gctcagtggc aacaacagtg ccgccacgcc
    20761 ggattcagca aagtggcgtg gctaccgcag gatggcagcc cgaccgccgg gatgagcgaa
    20821 catatcattc tcgccacgct gcccggtcag gcggttagcg ccgtaacatt caccgcgcca
    20881 tcagaacccg tgttggggca ggcgctgacg gataacggtg attatctcgc cgactggtct
    20941 gattgcgcag gtcagcccga acggtttaac gcccgctggc aggaggcatg gcgtctgctt
    21001 tcacagcgtc atggcgacgc tctgcctgtg gaaccgcccc ccgtcgccgc cccggagtgg
    21061 ctggggaagg ttcgcttaag ctggcaaaac gaagcctttt cccgcggtca gatgcgcgtt
    21121 gaagcccgtc atcctgctgg cgagtggctg ccgctatcgc ccgccgcgcc tcttcctgcg
    21181 ccgcaaacgc attatcaatg gcgctggacg cccctcaacg tcgccagcat tgaccatccg
    21241 cttaccttta gcttcagcgc cggtacgctt gcgcgcagcg acgagctggc gcaatacggc
    21301 atcattcacg atccgcacgc ctcttcacga ctgatgattg ttgaggagag cgaggatacg
    21361 ctggccttag cggagaaagt gatagcagcg ctcaccgcca gcgcagccgg attgattgtg
    21421 gttactcgcc gcgcgtggcg agtcgaggaa aatgaagcac tctctgcatc ccatcacgcg
    21481 ctatgggcct tgcttcgcgt cgcggccaac gaacagccgg aacggttgct tgccgccatc
    21541 gatctcgccg aaaacacccc gtgggaaacg ctgcatcaag ggttgagcgc agtctcacta
    21601 tcacagcgct ggctcgccgc acggggtgac accctttggc tcccttcact gacgcccaat
    21661 acgggatgcg ccgctgaatt accggcaaac gtgtttaccg gcgatagccg ctggcatctg
    21721 gtgaccggag cgtttggcgg attaggccgc cttgccgtga actggctcag agaaaaaggg
    21781 gcgcgacgca tcgccctgct ggcaccgcgc gtggatgagt catggctacg cgacgtggag
    21841 ggcgggcaga cgcgcgtctg ccgttgtgat gtgggcgatg ccgggcaact ggccacggtt
    21901 cttgacgatc tggcggccaa cggcggcatt gccggagcga ttcatgccgc tggcgtattg
    21961 gctgacgcgc ccttgcagga gcttgatgac caccagctgg ctgccgtttt cgcggtaaaa
    22021 gcgcaggcgg caagccaact gttgcaaacc ctgcgcaacc acgacggacg ctatcttatt
    22081 ctctactctt ccgctgccgc caccctcggc gcgccgggtc agagcgccca tgcgctggcc
    22141 tgcggctacc tggacgggct ggcccagcag ttttccaccc ttgatgcgcc gaaaacgctc
    22201 tctgtcgcct ggggcgcatg gggagaaagc ggtcgggcgg ccacgccgga aatgctggcg
    22261 acgctcgcca gccgaggtat gggcgcgtta agcgatgccg aaggctgctg gcacctggaa
    22321 caggcggtga tgcgcggcgc cccgtggcga ctggcgatgc gcgtttttac cgacaaaatg
    22381 cccccgttac aacaggctct gtttaacatc agcgccacag aaaaagccgc aacgccggtc
    22441 attcctcctg ctgatgacaa cgcctttaac ggcagcctga gcgatgaaac agcggtgatg
    22501 gcatggctga aaaagcggat tgcggttcag ctaaggctga gcgatccggc gtcactgcat
    22561 ccaaaccagg atctgttgca actcggcatg gactcgctgc tcttccttga actcagtagc
    22621 gatattcagc actacctggg tgtacgcatc aatgcggaac gggcgtggca ggatctgtct
    22681 cctcatggac tcacgcagct tatctgttct aagccagagg cgacgcctgc cgcttcgcag
    22741 ccggaagtat tgcggcacga cgccgacgag cgttatgcgc ccttcccttt gacgcccatt
    22801 cagcacgcct actggctggg gcgaacccac ctcattggct atggcggcgt cgcctgtcac
    22861 gtcctgtttg agtgggataa acgccacgat gagttcgatc tcgccatact ggagaaagca
    22921 tggaaccagc tcatcgcacg ccacgatatg ttgcgtatgg tggttgatgc cgacgggcag
    22981 cagcgaatcc tggcgacaac gccggagtat cacatcccgc gtgacgatct gcgcgcgctt
    23041 tccccggaag aacagcgcat cgcactggaa aaacggcggc atgaactgag ctatcgcgtt
    23101 ttgcctgccg accagtggcc tctttttgag ctggtggtca gcgaaatcga cgattgccat
    23161 taccgtctgc atatgaacct cgaccttttg cagtttgatg tgcagagttt taaagtcatg
    23221 atggacgacc tggcgcaggt ctggcgcggt gaaacgctgg cgccgctcgc tattaccttc
    23281 cgtgattatg tgatggctga acaggcgcgc cgacagacat cggcatggca cgatgcctgg
    23341 gattactggc aggaaaaact gccgcaactg cccttagcgc cagagctgcc ggtggttgag
    23401 acgcccccgg aaacgccaca cttcaccacc ttcaaatcga cgatcggcaa gacagaatgg
    23461 caggccgtga aacagcgctg gcagcagcaa ggcgtcacac cgtctgccgc gctgctcacg
    23521 ctgtttgccg ccacccttga gcgctggagc cgtaccacaa catttacgct gaacctgacg
    23581 ttcttcaatc gccagccgat ccatccgcaa atcaaccagt tgattggtga ttttacctcc
    23641 gtcacgctgg ttgattttaa cttctcagcg ccggtgacgt tgcaagagca gatgcaacag
    23701 acccaacagc gcctctggca aaacatggcg cacagtgaaa tgaacggtgt tgaggtgatc
    23761 cgtgagctgg gccgcctgcg cggatcacaa cgtcaaccgc tgatgccggt agtgtttacc
    23821 agtatgctgg ggatgacgct ggaaggcatg actatcgatc aggcgatgag ccatctgttc
    23881 ggcgaaccct gctatgtatt cacgcaaacg ccgcaggtct ggctggatca tcaggtcatg
    23941 gagagcgacg gcgagttgat gtttagctgg tactgcatgg acaacgtgct ggaacccggc
    24001 gctgccgagg cgatgtttaa tgactattgc gccatcctgc aagccgtcat cgccgcccct
    24061 gaaagcctga agactctcgc cagcggcatc gccaggcaca ttccccgccg acgctggccg
    24121 ctgaacgcgc aggcggacta cgacctgcgg gatattgagc aggcgacgct cgaatacccc
    24181 ggcatccggc aggccagagc ggaaataacc gaacagggcg cgttgacgct ggatatcgtg
    24241 atggccgacg atccttcgcc atcagcggcg atgcctgatg agcacgaact tacccaactg
    24301 gcgctgccgt tgcctgagca ggcgcagctt gatgagctgg aggcgacctg gcgctggctg
    24361 gaggcgcgtg cgctacaggg gatcgcggct acgctaaatc gtcacggcct gtttaccacg
    24421 ccggagatcg cccatcgctt tagcgcagta gtacaggcgc tgtccgcgca agcgtctcac
    24481 cagcgtctgc tgcgccagtg gctacagtgt ctgacggaaa gagagtggtt aatccgcgaa
    24541 ggtgaaagct ggcgctgccg cattccgctc agcgagattc ctgagcctca ggaagcgtgc
    24601 ccgcaaagcc aatggagcca ggcgctggcg cagtatctgg aaacctgcat cgcccggcac
    24661 gacgccctct tctccgggca gtgttctccg ctggaattgc tgttcaacga gcagcatcgc
    24721 gttaccgacg cgctgtatcg cgacaacccc gccagcgcct gtctgaatcg ctataccgcg
    24781 cagattgccg ccttgtgcag cgcagaacgg attctggagg ttggcgccgg aaccgcagcc
    24841 actaccgcgc cggtgctgaa ggccacgcgg aacacgcggc agtcgtacca cttcacggac
    24901 gtctccgcgc agttcctcaa tgacgccaga gcccgtttcc atgatgaatc gcaggtgtct
    24961 tatgccttgt tcgacatcaa ccagccgctg gatttcaccg cccacccgga ggcgggttac
    25021 gacctgatcg ttgccgtcaa tgtgctccac gacgccagcc atgtcgtcca gacgttgcgc
    25081 agattaaaac tgttgctgaa agccggcgga cgtttgctga tcgttgaagc gacggagcga
    25141 aacagcgtat tccagctggc gagcgtgggc tttattgagg gattaagcgg ataccgcgat
    25201 ttccgccgcc gggatgagaa accgatgctc acccgctccg catggcagga ggttctcgtt
    25261 caggccgggt ttgcaaacga gctggcgtgg cccgcgcagg aatcgtcgcc gctgcgccag
    25321 catctgctgg tagcgcgttc gcctggcgta aatcgcccgg ataaaaaagc cgtgagccgc
    25381 tatttacagc agcgctttgg caccggtctg cccattttac agatccggca aagagaagcg
    25441 ttatttacgc cgctgcatgc cccgtctgat gcgccgactg agccagccaa acccacgcca
    25501 gttgccgggg ggaatccggc actggaaaaa caggtggctg aactctggca atcgctgctg
    25561 tctcgccccg tggcaaggca tcacgacttt ttcgaactgg gcggcgacag cctgatggcg
    25621 acaaggatgg tcgcgcagct aaaccggaga gggattgcca gggctaacct tcaggatctg
    25681 ttcagccatt cgacgctgag cgacttctgc gcccatctac aggcggctac gtcaggagag
    25741 gacaacccga tacccctttg ccagggcgac ggtgaggaaa ccctgtttgt cttccacgct
    25801 tcagacggcg atatcagcgc ctggctgccg ctcgctagcg cgttgaacag gcgcgttttc
    25861 ggcctgcaag caaaatcgcc gcagcgcttt gccacgctcg accagatgat cgatgagtat
    25921 gtcgggtgca tccgtcgtca gcagcctcac ggcccttatg tgctggcggg ttggtcgtat
    25981 ggcgcgtttc ttgcggcggg cgccgcacaa cgcctgtacg ccaaaggcga gcaggttcgg
    26041 atggtgttaa tcgatcccgt gtgccgacag gatttctgtt gcgaaaaccg ggcggccctg
    26101 ctgcgcctgt tagccgaagg acaaacgcct ctggcactgc ccgaacattt cgaccagcag
    26161 acgcccgaca gccagcttgc cgactttatc agcctcgcta aaacggccgg tatggtgtcg
    26221 caaaacctga cgctgcaagc ggcagaaacg tggctcgaca acatcgcgca tctgctgcgt
    26281 ttactgactg agcatacgcc gggcgaaagc gttccggtcc cctgtctcat ggtgtatgcc
    26341 gccgggagac ccgcgcactg gacgccagca gaaaccgagt ggcagggctg gataaacaac
    26401 gccgacgacg ctgtgattga agccagccac tggcaaatca tgatggaagc cccccacgtt
    26461 caggcttgtg cgcaacacat tacgcgctgg ctttgcgcaa cctcaacgca accggagaac
    26521 acgttatgat gccgtccgcc tccccaaaac aacgcgtact gattgtgggc gccaaatttg
    26581 gcgaaatgta cctgaatgcc tttatgcagc ccccggaggg gctggaactg gtcggcctgc
    26641 tggcgcaggg aagcgcccgt tcaagagagc tggctcatgc gtttggcatt ccgctgtata
    26701 cctcgccgga acagataacc aggatgccgg atatcgcctg tattgtcgtg cgttcgaccg
    26761 tcgccggagg aaccggtacg cagcttgcca gacactttct gacgcgcggc gtacacgtca
    26821 ttcaggagca tcccctccac ccggatgaca tcagttcctt acaaacgctg gcgcaggaac
    26881 agggctgctg ctattgggta aacacgtttt atccgcatac gcgcgcggga cgcacatggc
    26941 ttcgcgatgc gcagcagctg cgtcgctgtc tggctaagac gccgcaggtg gtccacgcca
    27001 ccaccagccg acagttgctc tactccactc tggatttgtt gttgctggct ctgggcgttg
    27061 atgccgccgc cgtggagtgc gacgtggttg gcagctttag cgattttcac tgtctgagac
    27121 tcttctggcc ggagggagaa gcctgcctgc tgcttcagcg ctatctcgat cctgacgatc
    27181 cagatatgca tagccttatc atgcaccgcc tgctgctggg ctggccggaa ggtcatcttt
    27241 cgctggaggc gagctacggc cccgtcatct ggtcatccag cctgtttgtc gcagaccatc
    27301 aggagaacgc ccacagcctc taccgcagac cggagatcct gcgcgatctg ccgggtctga
    27361 cgcgcagcgc ggcgcccctg agctggcgcg actgctgcga aaccgtcggg ccggagggcg
    27421 tcagctggtt gctacaccag ttgcgcagcc atctggcggg cgaacatcca ccagcggcct
    27481 gccaaagcgt acatcagatc gcgttatcac gtttatggca gcagatttta cgtaagaccg
    27541 gcaatgcgga gatcagacgc ctgacgccgc cgcaccacga ccggttagcc ggtttctaca
    27601 acgatgatga taaggaggcg ctgtgacgca atctgcaatg tgcatcccgc tgtggcccgc
    27661 ccggaacggc aatactgcgc atctggtcat gtgccctttc gctggcggca gcagtagcgc
    27721 gtttcgccac tggcaagctg agcaactggc tgattgcgcg ctttctctgg tgacctggcc
    27781 ggggcgcgat cgccttcgcc atctggaacc gctcagaaac attacacaac tggcggcact
    27841 gctggcgaac gagctggaag catccgtatc gcctgacacg ccgcttttac tcgccgggca
    27901 cagcatgggg gcgcaggtgg cgtttgaaac ctgccgactt ctggagcaac gggggcttgc
    27961 gccacaagga ctgattattt ccgggtgcca tgccccgcat ctgcattctg aacgccagct
    28021 cagccatcgc gatgatgccg actttatcgc tgagctgata gacattggcg gatgttctcc
    28081 tgaactgcgg gaaaaccagg aattaatgtc gctgtttctt cctcttctgc gcgctgattt
    28141 ttacgccacc gagagctatc actacgactc gcccgacgtc tgtccgccgc tgcgcacgcc
    28201 tgcgctgtta ttgtgcggca gccacgatcg cgaagcctcc tggcagcagg tcgatgcctg
    28261 gcgtcagtgg ctgagccacg ttacaggccc ggtggtgatt gacggcgatc atttctatcc
    28321 cattcaacaa gcccggtcct tttttacgca gattgtccgc cattttcccc acgcattttc
    28381 tgcaatgacc gcgtggcaaa aacagcccag tacttcagaa aggtgacgca tgaattcttc
    28441 ctttgaatct ctgattgaac agtatccctt acccattgcc gaacagttgc gccactgggc
    28501 ggcccgttat gcctcgcgaa ttgccgtcgt tgatgcaaag gggtcgttaa cctacagcgc
    28561 gcttgatgca caagttgacg aacttgccgc aggtctgtca tcactgggtt tgcgttcggg
    28621 ggagcatgta attgtgcagc ttcccaacga caacgcgttt gttaccctgc tgttcgcctt
    28681 gttaagactg ggcgttatcc ccgtgctggc gatgccctcg caacgggctc tggatatcga
    28741 cgcgctgatt gagctggcgc aacccgtcgc ttacgttatt cacggggaaa acctcgcaga
    28801 gctggcccga cagatggcgc acaaacacgc ctgcttgcgt catgttctgg tcgctggaga
    28861 gaccgtgagc gacgatttta cgccgctctt ctcccttcac ggtgagcgac aggcatggcc
    28921 gcagcctgat gtttccgcca ccgcgttgtt gttgctctca ggcggcacaa ccggcacgcc
    28981 caaactcatc ccgcgccgac atgccgacta tagctataac ttcagcgctt ctgctgaact
    29041 gtgcggcatc agccaacaga gcgtgtatct cgccgtcctc ccggtggcgc ataactttcc
    29101 gctggcctgc cccggtattc tgggaacgct tgcctgcggc ggaaaagtgg tgctgaccga
    29161 cagcgccagc tgtgatgagg tgatgccttt aatcgcgcag gaaagagtga ctcacgtcgc
    29221 cctggttccg gcgctggcgc aattatgggt gcaggccagg gagtgggaag acagcgacct
    29281 ttcgtcgctg cgcgtcattc aggcaggcgg cgcccggctc gacccgacgc ttgctgagca
    29341 ggttatcgcc acctttgact gtaccctgca acaggttttc ggtatggcgg aaggcctgct
    29401 ctgttttacc cggctggacg atccgcatgc caccattctc cacagccagg ggcgcccgtt
    29461 gtcccctctg gatgaaatcc gcatcgttga tcaagacgag aacgacgtcg cgccgggcga
    29521 aaccgggcaa ttgttaacgc gcggccctta taccatttcg ggctattacc gcgcccctgc
    29581 ccacaacgcg caggccttta ccgcgcaagg gttttaccgc acaggcgaca atgtcaggct
    29641 ggatgaggtg gggaacctgc acgttgaggg acgcataaaa gagcagatca accgcgccgg
    29701 agaaaaaata gccgcggctg aagtggaatc ggcactgctg cgtttagcgg aagtgcaaga
    29761 ttgcgcggtg gtcgccgcgc cggacacgct gcttggcgag cggatttgcg cgtttatcat
    29821 cgcgcagcag gtgccaactg actatcagca gttgcgtcaa caactgaccc gtatggggct
    29881 cagcgcgtgg aaaattcctg accaaatcga gtttctggac cactggccgc tcaccgccgt
    29941 cggcaagata gacaaaaaac gcctgacggc tctcgccgtc gaccgttatc gccattctgc
    30001 ccaataagcg caaaccgacc cgaaacaggt tgaaataaac ccgtttcggg tagcaccact
    30061 attagaaata gttatcattt tcaattcacc attgtcggta tttttggcgt ttcgccgtct
    30121 tacagggact cacaacaatg aaaatgacac ggctttatcc tctggccttg gggggattat
    30181 tgctccccgc cattgctaat gcccagactt cacagcaaga cgaaagcacg ctggtggtta
    30241 ccgccagtaa acaatcttcc cgctcggcat cagccaacaa cgtctcatct actgttgtca
    30301 gcgcgccgga attaagcgac gccggcgtca ccgccagcga caaactcccc agagtcttgc
    30361 ccgggctcaa tattgaaaat agcggcaaca tgcttttttc gacgatctcg ctacgcggcg
    30421 tctcttcagc gcaggacttc tataaccccg ccgtcaccct gtatgtcgat ggcgtccctc
    30481 agctttccac caacaccatc caggcgctta ccgatgtgca aagcgtggag ttgctgcgag
    30541 gcccacaggg aacgttatat ggcaaaagcg ctcagggcgg gatcatcaac atcgtcaccc
    30601 agcagccgga cagcacgccg cgcggctata ttgaaggcgg cgtcagtagc cgcgacagtt
    30661 atcgaagtaa gttcaacctg agcggcccca ttcaggatgg cctgctgtac ggcagcgtca
    30721 ccctgttacg ccaagttgat gacggcgaca tgattaaccc cgcgacggga agcgatgact
    30781 taggcggcac ccgcgccagc atagggaatg tgaaactgcg tctggcgccg gacgatcagc
    30841 cctgggaaat gggctttgcc gcctcacgcg aatgtacccg cgccacccag gacgcctatg
    30901 tgggatggaa tgatattaag ggccgtaagc tgtcgatcag cgatggttca ccagacccgt
    30961 acatgcggcg ctgcactgac agccagaccc tgagtgggaa atacaccacc gatgactggg
    31021 ttttcaacct gatcagcgcc tggcagcagc agcattattc gcgcaccttc ccttccggtt
    31081 cgttaatcgt caatatgcct cagcgctgga atcaggatgt gcaggagctg cgcgctgcaa
    31141 ccctgggcga tgcgcgtacc gttgatatgg tgtttgggct gtaccggcag aacacccgcg
    31201 agaagttaaa ttcagcctac gacatgccga caatgcctta tttaagcagt accggctata
    31261 ccaccgctga aacgctggcc gcatacagtg acctgacctg gcatttaacc gatcgttttg
    31321 atatcggcgg cggcgtgcgc ttctcgcatg ataaatccag tacacaatat cacggcagca
    31381 tgctcggcaa cccgtttggc gaccagggta agagcaatga cgatcaggtg ctcgggcagc
    31441 tatccgcagg ctatatgctg accgatgact ggagagtgta tacccgtgta gcccagggat
    31501 ataaaccttc cgggtacaac atcgtgccta ctgcgggtct tgatgccaaa ccgttcgtcg
    31561 ccgagaaatc catcaactat gaacttggca cccgctacga aaccgctgac gtcacgctgc
    31621 aagccgcgac gttttatacc cacaccaaag acatgcagct ttactctggc ccggtcggga
    31681 tgcagacatt aagcaatgcg ggtaaagccg acgccaccgg cgttgagctt gaagcgaagt
    31741 ggcggtttgc gccaggctgg tcatgggata tcaatggcaa cgtgatccgt tccgaattca
    31801 ccaatgacag tgagttgtat cacggtaacc gggtgccgtt cgtaccacgt tatggcgcgg
    31861 gaagcagcgt gaacggcgtg attgatacgc gctatggcgc actgatgccc cgactggcgg
    31921 ttaatctggt cgggccgcat tatttcgatg gcgacaacca gttgcggcaa ggcacctatg
    31981 ccaccctgga cagcagcctg ggctggcagg cgactgaacg gatgaacatt tccgtctatg
    32041 tcgataacct gttcgaccgt cgttaccgta cctatggcta catgaacggc agcagcgccg
    32101 tcgcgcaggt caatatgggt cgcaccgtcg gtatcaatac gcgaattgat ttcttctgat
    32161 tattgtaaaa gggataccga aaaggtatcc cttttacacc actagttaaa accagtaact
    32221 cagcagagtc gcaaaaatat taatccatag tgattattta aacaatgaaa ttgcgattag
    32281 gataaataga tttaa
//
LOCUS       DACADA010000038        32065 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.038, whole genome shotgun sequence.
ACCESSION   DACADA010000038 DACADA010000000
VERSION     DACADA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.038"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..871
                     /locus_tag="ISB17_001754"
     CDS             <1..871
                     /locus_tag="ISB17_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP1979695.1"
                     /translation="NIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKI
                     EQRIAHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSD
                     IVDCDFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQ
                     VFKTWGENAVSEDEVDYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFP
                     EWTIVDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVIT
                     EKLHHATSRVVEI"
     gene            898..1368
                     /locus_tag="ISB17_001755"
     CDS             898..1368
                     /locus_tag="ISB17_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP1979696.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            1386..1700
                     /locus_tag="ISB17_001756"
     CDS             1386..1700
                     /locus_tag="ISB17_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP1979697.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            1725..2816
                     /locus_tag="ISB17_001757"
     CDS             1725..2816
                     /locus_tag="ISB17_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP1979698.1"
                     /translation="MKENKITVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            2828..3679
                     /locus_tag="ISB17_001758"
     CDS             2828..3679
                     /locus_tag="ISB17_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP1979699.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            3760..4620
                     /locus_tag="ISB17_001759"
     CDS             3760..4620
                     /locus_tag="ISB17_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP1979700.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            4668..5600
                     /locus_tag="ISB17_001760"
     CDS             4668..5600
                     /locus_tag="ISB17_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1979701.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            5581..6261
                     /locus_tag="ISB17_001761"
     CDS             5581..6261
                     /locus_tag="ISB17_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP1979702.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(6305..8014)
                     /locus_tag="ISB17_001762"
     CDS             complement(6305..8014)
                     /locus_tag="ISB17_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP1979703.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLKNVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFAPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(8135..9526)
                     /gene="xanP"
                     /locus_tag="ISB17_001763"
     CDS             complement(8135..9526)
                     /gene="xanP"
                     /locus_tag="ISB17_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAP1979704.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9806..11011
                     /gene="gltS"
                     /locus_tag="ISB17_001764"
     CDS             9806..11011
                     /gene="gltS"
                     /locus_tag="ISB17_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAP1979705.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(11045..13126)
                     /gene="recG"
                     /locus_tag="ISB17_001765"
     CDS             complement(11045..13126)
                     /gene="recG"
                     /locus_tag="ISB17_001765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP1979706.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13132..13821)
                     /gene="trmH"
                     /locus_tag="ISB17_001766"
     CDS             complement(13132..13821)
                     /gene="trmH"
                     /locus_tag="ISB17_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAP1979707.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(13828..15936)
                     /gene="spoT"
                     /locus_tag="ISB17_001767"
     CDS             complement(13828..15936)
                     /gene="spoT"
                     /locus_tag="ISB17_001767"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP1979708.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15955..16230)
                     /gene="rpoZ"
                     /locus_tag="ISB17_001768"
     CDS             complement(15955..16230)
                     /gene="rpoZ"
                     /locus_tag="ISB17_001768"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP1979709.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16285..16908)
                     /gene="gmk"
                     /locus_tag="ISB17_001769"
     CDS             complement(16285..16908)
                     /gene="gmk"
                     /locus_tag="ISB17_001769"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP1979710.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17166..18848
                     /gene="ligB"
                     /locus_tag="ISB17_001770"
     CDS             17166..18848
                     /gene="ligB"
                     /locus_tag="ISB17_001770"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAP1979711.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18845..19462)
                     /locus_tag="ISB17_001771"
     CDS             complement(18845..19462)
                     /locus_tag="ISB17_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAP1979712.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19714..20577)
                     /locus_tag="ISB17_001772"
     CDS             complement(19714..20577)
                     /locus_tag="ISB17_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP1979713.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20704..21420
                     /gene="rph"
                     /locus_tag="ISB17_001773"
     CDS             20704..21420
                     /gene="rph"
                     /locus_tag="ISB17_001773"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP1979714.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21486..22127
                     /gene="pyrE"
                     /locus_tag="ISB17_001774"
     CDS             21486..22127
                     /gene="pyrE"
                     /locus_tag="ISB17_001774"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP1979715.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22164..22760)
                     /gene="slmA"
                     /locus_tag="ISB17_001775"
     CDS             complement(22164..22760)
                     /gene="slmA"
                     /locus_tag="ISB17_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAP1979716.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22867..23325)
                     /gene="dut"
                     /locus_tag="ISB17_001776"
     CDS             complement(22867..23325)
                     /gene="dut"
                     /locus_tag="ISB17_001776"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP1979717.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23303..24523)
                     /gene="coaBC"
                     /locus_tag="ISB17_001777"
     CDS             complement(23303..24523)
                     /gene="coaBC"
                     /locus_tag="ISB17_001777"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAP1979718.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24695..25363
                     /locus_tag="ISB17_001778"
     CDS             24695..25363
                     /locus_tag="ISB17_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP1979719.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25580..25816
                     /gene="rpmB"
                     /locus_tag="ISB17_001779"
     CDS             25580..25816
                     /gene="rpmB"
                     /locus_tag="ISB17_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP1979720.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25837..26004
                     /gene="rpmG"
                     /locus_tag="ISB17_001780"
     CDS             25837..26004
                     /gene="rpmG"
                     /locus_tag="ISB17_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP1979721.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26102..26911
                     /gene="mutM"
                     /locus_tag="ISB17_001781"
     CDS             26102..26911
                     /gene="mutM"
                     /locus_tag="ISB17_001781"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAP1979722.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26950..27429)
                     /gene="coaD"
                     /locus_tag="ISB17_001782"
     CDS             complement(26950..27429)
                     /gene="coaD"
                     /locus_tag="ISB17_001782"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP1979723.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27437..28714)
                     /gene="waaA"
                     /locus_tag="ISB17_001783"
     CDS             complement(27437..28714)
                     /gene="waaA"
                     /locus_tag="ISB17_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAP1979724.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29128..30186
                     /gene="rfaQ"
                     /locus_tag="ISB17_001784"
     CDS             29128..30186
                     /gene="rfaQ"
                     /locus_tag="ISB17_001784"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAP1979725.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30183..31307
                     /locus_tag="ISB17_001785"
     CDS             30183..31307
                     /locus_tag="ISB17_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP1979726.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31300..>32065
                     /gene="rfaP"
                     /locus_tag="ISB17_001786"
     CDS             31300..>32065
                     /gene="rfaP"
                     /locus_tag="ISB17_001786"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAP1979727.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLLNMASVKAEH"
BASE COUNT         7634 a         7924 c         8629 g         7878 t
ORIGIN      
        1 taacattttt actcatatcc ttattatgat gtatcgcaac acgcacggga atgcgttatc
       61 aagagaagaa gatcaaacca ggcaatatga tgaaaatatc tttaatgttg ccagtcagat
      121 gattcataag atagaacaac gaattgcaca tacattgccc gatgatgaag tctggtttat
      181 ttatcaatat atcatttcat caggtgtggc gattgatgga caaaaagatg ttagcattat
      241 ttcacatatg caggccagca atgaagcgcg tctgattacc tggcgtttaa ttacggtatt
      301 cagtgacatc gtggactgcg attttagtga agacagcgca ttatatgatg gcttattggt
      361 gcatattaaa ccgctgatta accgactaaa ttatcgtatt catatccgta atccattgct
      421 ggaagatatt aaagcagaat tagcggatgt ctggcggttg acgcaatatg tggtgaatca
      481 ggtatttaaa acctggggtg agaatgcggt gagcgaggat gaagtggatt acctgaccgt
      541 tcattttcag gctgcgatgg aacggcaaat tgcccgtaaa cgcgtattac tggtctgttc
      601 aaccggaatc ggaacttcgc atctactgaa aagccgtatt ctgcgagcat ttcctgaatg
      661 gacgattgtt gatgttattt cagcagcgaa tttatcacag gttttgcctg acaatatcga
      721 actgattatt tcgaccatta atttgcctac agtcactatg ccggtcgctt atgttaccgc
      781 tttttttaat gatgccgata ttaagcgggt cactgaaatg gtgattacgg aaaaattaca
      841 tcatgcgacg tctcgggtcg ttgaaattta atgtcagaat tttatgaggt atgacgaatg
      901 gacataacaa aaatcctgaa tacgaatcgc gttattttag atatgcaagc gacgaataaa
      961 gcggaggcga ttgaagaatt aactaatctt ctcaaaaaag atggtgcgat cgattgtcgg
     1021 gaaaccttta ttaaagatgt ctggcagcgc gaagcggaag gctcgactgg ttttgaaaat
     1081 catattgcga tcccgcatgg taaatcttca gctgtgatta atactacgct ggcaattggc
     1141 cgtacccgcc aggatattcc ctgggaaacg ctggatggca gtagcgtgcg ctgtattatt
     1201 ttatttgcgg tacgtcttga agatcaaaat accacccata tccgtttact ttcccaggtc
     1261 gcgggcgcgc tggcggatga tgacatcata gaacaattgt tagttgagac gagtccgcaa
     1321 aaaatcattg atctctttag tcagtatgca caatccgatg tctgctaaat aacagggagt
     1381 atgtcatgaa tattgtctgt gttgccgcct gtacggcggg tattgctcat acctatattg
     1441 ctcgtgaaaa gttaatcaaa ggagcaaaag ctctgggcca taccattaaa gttgaaaccc
     1501 agggaaccat tggtacagaa aatgaattac aggcggcaga tatctctgct gctgacgtcg
     1561 tgattcttgc tgtagatgtg aaaataaaag gagaagaacg cttcaccaat aaacgtattg
     1621 tgcgagtaaa gacagagatc gtcattaagt cgcccgttca atttcttgaa aaggtcgaaa
     1681 aatcactggg aaacaaatga tccttatact ggagggatct taatatgaaa gaaaataaaa
     1741 ttacggtttc acaggaaatt aaaaaacatt tattaaccgg catctcgtgg atgatacccc
     1801 ttattgtggc cgccgggata tgtattgcgt tggggcaagt tcttggcggt acagatgttg
     1861 gtgaaaaaac aggaacaatc ccgtggatgc ttaatcagat tggcggctgg ggaatggggc
     1921 tgattgtgcc gctgattagc gcggcaattg cctattctat tgccgatcgt cccggatttg
     1981 ccccgggcct gattgtcggc tttctctgtg ggcaaatcca taccggattt attggcggta
     2041 tgttgggtgg tttcctggtg ggctacacca ttttgctact taaacgctat atccgcctgc
     2101 cgcagtcgat gcaaggactc atgccaatca tggtgttgcc ggtattaagc accattatcg
     2161 gtgggctgtt aatgatgacg ctgattggta aacccatcgc ctggttacaa gaagcactga
     2221 ttcatttgct cgagtcgatg cagggcggat cgcgcttcct gatgggggcc atacttggcg
     2281 cgatggcaac cttcgatttc ggtggtccgg tcaacaaaac catgtcgctg ttttccgatg
     2341 gcttgctggt cagtggcgtc tacggaccag aagccgtgaa atttgtcggc tcaatcatcc
     2401 ctccgtttgg catcacactt tcgttcttac tgacacgcca taaatacacc cgtgcggagc
     2461 gcgaggcgct gaaagccgcg tttccgatgg ggatctgcat gattactgaa ggagtcattc
     2521 cgatcgccgc gcgtgaccta ctgcgagtag tgggatcttg tgtggtggca tcggcggtcg
     2581 cgggcggctt aatcatggtg tggggcgtgg agagtccggt tccgcatggc ggcatgtttg
     2641 ttgtgcctct gttcacccat cctctgctgt tttgcctggc gctgggtata ggcacggtga
     2701 tttgtggcgt gatgctttca ctctggaaga aaccggtaac ggaacgcgac gaagagtttg
     2761 acgaactgag cgatcaaaag ctgaaagatg aagagatcac tttcaccctc gaataaccgg
     2821 aggccctatg tttgccgata tgaaaagtat ggtgatgaaa gcctggcatg aacattacgc
     2881 cttgctggcg atcaactgta tgaatctgga aagtgctcac gcagctatcc gcgtggcgga
     2941 gaagaatcgc gctccgatca ttctcaatct ctatcagggg catctggcgc attttcctgc
     3001 acccgtcgcg gcggcagtgg ttaaaacgct cgcggaatct gcctcagtac cggtcgctct
     3061 ggcgcttgat catggcaaaa atccggactg tattcgtcag gcgtttcgtg ctggtttcag
     3121 cggattaatg attgatgctt ctgcttttcc cctggaggaa aacgtgcgcc agaccagggc
     3181 ggtggtggaa ctcgccgcca gcgcgcagct ctgcgtggaa ggcgaactgg gccatctggc
     3241 agatgcgccg cgttacgacc aggccgccaa tgcagacctg atgacgcaac ccactgatgt
     3301 ggaaccgttt atcgcgcaga caggcattga tctactggcc gtctcggtag gcacggcgca
     3361 cggcatgtac gcgcctggcg ttgtacctgc tattgatttt cagcgtctgg cggagatttt
     3421 ccactgctcg agcgtgcctc tggcattaca tggcggtagc ggtacgccgt tcgatcagct
     3481 ccagctctgt acgactctgg gggtagcaaa aatcaacgtg ggggcggcca tttttgaacg
     3541 ggggaaatcc gcgctcctgc ataccctctg tcacgatata tccattgaac tggtggacgc
     3601 gctgaaagct atggaactgg ccttcgaaga agcgattaca ccgtatttac aggcgagcgg
     3661 cgctatcggc aaagcctgat tcattttctg tcatggcctt tatgccagtg caataacgca
     3721 ccggcagggc ggactacacg ctgaaactgg agatttaaca tgttagtttc gatgaaagaa
     3781 ttactgcaac ctacccgcca gcacggcttt gccattggcg catttaatgt ggcggataac
     3841 tgtttccttc gcgcagtcgt ggatgaagca gaagcgacaa atactccggc gattattgcc
     3901 atccatccca gcgaacatga ttttgtgggc gatgcctttt tcgctcacgt tcgcgaaatt
     3961 acccagcgta gtccggtccc gtttgtactg cacctcgatc atggtgcctc tgtagagcat
     4021 gttttgcgtg cgattcgctg cggatttacc tcagtaatga tcgacggttc gctgctgccg
     4081 tatgaagaaa acgtggcgct gaccgcagaa gtggttcgcc tcgctcacgc ggttggcgtg
     4141 tcggttgagg gtgaactggg aaccatcgga caaacaggaa cgtcggtaga aggtggtgtt
     4201 tcacaagtga cctacacaga tccggcgcag gccgccgatt ttattgctcg tacaggtgcc
     4261 gacacactgg ctgtggcgat tggtacagcg catggcatct atccaaaagg gatgcagccg
     4321 aagctgcaaa tggacatttt gcgcgacatt gccggacgta ttgatattcc gctggtgcta
     4381 cacggtggtt cggcaaaccc ggacgcggag attgctgaat cagttacgct cggcgtcggc
     4441 aaaatcaata tctccagcga tatgaaatac gcctatttcc agaaagtgcg tgaaattctc
     4501 gctaaagaga gctggtggga cccgaacgtc atctatccgg atgcgattaa cgcggcgcgg
     4561 gaagtcattc gccacaaaat gaaactgttc ggttcgctcg gtaaagcatc actgtactaa
     4621 gccagaaggg cggggtgacc cgccttttac gcaacaagga acgagatatg cactatctgg
     4681 ggttggatat tggtgggacc aaaatcgccg ccgttgtcat ggatgcgcat ggctgggaga
     4741 ttcgccgtta ccgctgcccg acgcaaaagt cgacatatca acaatttgtc tcatgcgttg
     4801 tggcgcttat cgagcagatt aggcgggacg ttcaacgacc gatgctgaca gggatcgcct
     4861 tacccggcag tatctcgcca ctcactggcc tgattaaaaa cgcgaatatt caggtgatta
     4921 acggtcatgc gttacaggct gatttgcagc aattgcttgg gcaaccggtg gtgatagcca
     4981 atgatggtaa ctgtttcgcg ctatcagaag cttgcgacgg tgccgggcaa gattatgacg
     5041 tggtatttgg tattacgctt ggtacgggct gcggcggtgg cattgccatc aagcaacgac
     5101 cgtttatagg ggcctgggga aatgctgccg aatgcggtca tatcacgttg ccaggctata
     5161 cggagcagga agatggtcca tcagtcagtt gctattgcgg caaacacaac tgcgtggagt
     5221 cgtttgtttc cggcagcggt tttagtgaac gctatcaaca gatgactggt aacttgctca
     5281 ctcctgcggc gattgtcacc ctggcacaac gtggtgatgc ttgtgccatg cagcaggtgg
     5341 cacgttttcg ccaacagctt gcccgcacgc tggcaaccat cgttaacgtt gttgaccctg
     5401 gcgtgattgt gatcggcggc gggctttcga atgtggaact gcttatcacc gatctgaacg
     5461 cagaagtcgc tccactggtt ttcaccgatc aattcaccac tcccattgta aaagcacagc
     5521 acggcgacag tagcggaatg cgtggcgctg cctggcttgc tatgcgcaac ggagaagcca
     5581 atgaaacgtt cacaaattaa ttatgttatt gataaagcac atgccattgc gcagaccttc
     5641 cgcgtttgtt tgccagagtt cgcctatttt acggtggatg actggctgca gcgggaacgg
     5701 gataactggc aagaagtggt cgacttacag ctcggttggg atatcaccga ttttggtcgt
     5761 ggtgatttca accaaacggg actaacactg ctaacgatgc gcaacggcgc gctggggtcg
     5821 gcggcatatc caaaaccgta tgctgagaaa atgttgcaga tccaacaaga tcagcagact
     5881 ccctggcatt ttcacaccca caaaatggaa gatatcctca atcggggcgg tggagactta
     5941 tgcatgcagt tggcctgggc caacgaagcc aatctctgtg atgatcaacg agtcattacc
     6001 gtgagtgttg atggtcagcg gcgtaccatg aaacctggtg aaacgctggt gctaaagccg
     6061 gggcagggca tttgcctgcc gccgcgtctg tatcatcgct tctgggcgga aaaagcgttt
     6121 gttctcggct gggaaatctc gatggtaaat gatgaccaac acgataatta ttttcttgag
     6181 ccgggaggac gttttccggc gatagaagaa gatgaaccgg tgaaatggtt attgtgcggt
     6241 gagtatggaa ttttgcgcta ataacgggga acgctagcgc tgatatccgc tagcgtcctt
     6301 atttttactg caccggctcc gtcgacgtaa cttcaccact ggaaactacg cccgcattca
     6361 tggtttgagt cacttgctgc tttgcggcat tcacggcatg caattgcccg ctaaccgtag
     6421 gtttcagcgg gacattgacc tgaatatcgc cactggcggt aagctgaata ttgccgtcgc
     6481 cagtcaacgg taattcaggc catccccaat gttgcaaaat attcaccggc acaccgcgtc
     6541 cattcaggct gatatgggtc tgacgttgtg gcgtttgtga aacactggcg gtggcttcca
     6601 gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcag
     6661 ttagcgccag cgacggacga cggacatcaa cacgattaaa cgttgcggcg gcggcattca
     6721 gattcgccga gccactccag acgccccatt tatgatcggt aaccagcgtc agattggcac
     6781 cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat
     6841 tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca
     6901 tccacaactg ttgccagttt ttgggcaagg tatattccag tccggcaatt gccgcatcat
     6961 cgaggatcag cgttttcccg tcgcgttgcc agtttcctga cgttctgacc ataccccctt
     7021 cccagcggct ggtgaactgg cgcagtgcta cgccctgcgg ggcaaattcc gcgtttataa
     7081 tcggatcaaa taaatgcagc gagccataaa taaactcgct ggcgttcatc gacagtttac
     7141 cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt
     7201 cggccaccgc ccagtccgga ccttgcaaac gagcgtcgat gacttccagg cgaccaatct
     7261 gcaacgacgg gacagagcgt aatggtgcaa agaagtcggt tagcgatttt tcgctttgta
     7321 gacggatatc cgccatgcgc aggttttcca cttgccagct gccgtcagcg ttacgctgcg
     7381 cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc gtaacgcgat
     7441 cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg
     7501 atccggcact aaactgaatc tgtgccttcg tacccagcac tttaccggct tctggtgacc
     7561 acggaaccac gccgccattt acccgctgcg cgctcagttt ccattcgcta ttcgggctat
     7621 taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt
     7681 cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt ggttcggtca
     7741 gttgccgact gcttagcgca atgtcgacac tttttgctac cagggtcgcg ggctggccat
     7801 cacggccaaa cgtgacgttc ttcagcacga tatgagatgg cgcggaaaaa cggtgatcca
     7861 tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg atatgttctg
     7921 ctccccagcg agtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga
     7981 gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaatgcacat
     8041 aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga
     8101 ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca
     8161 gattcagcac gatggcagta ataccgcccg cggcaatccc ggaggagagc aggtttttca
     8221 gccattcagg ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga ccgaccgcca
     8281 gcgacagcgc gataatcaga atcgcccggc ggttcagcgg ctcacgagaa acgatacgca
     8341 caccggaggc ggcgatggtg ccaaacatta caagcgttgc gccgcccaga accggttctg
     8401 gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc aacatcagcg
     8461 cgacgacaaa accgacatag cggctggcaa caccggttaa ctggatcacc ccgttgttct
     8521 gcccgaagca ggagttcggg aaggtattaa ataccgccga gacaaacgag ttcaggccgt
     8581 ttgccagtac gccgcctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg
     8641 aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca
     8701 gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc ggcgttggca
     8761 ccataatcag ttcttgtgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg
     8821 ccagcgcata tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag taagggttac
     8881 gttgacggtt aagcaggata attaaggcta agaccacgcc tgccagcagc agatttttcg
     8941 gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggag gtcagtccaa
     9001 cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc ggcgtaataa
     9061 tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtacaactt gccagcatca
     9121 acgtgccgaa caaagctgcc atcatggtag gaacatcagc accaccggtc ttcagcgctg
     9181 tgccgcccat aatcagcgga gcaacaaagt tgaagctggt gccctgaata gacaacaacc
     9241 cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca
     9301 gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta
     9361 atagcgctgg cgtgatcacc gcaacgaaca tcgccagcaa atgctgacag gcggcaaaca
     9421 gagtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct
     9481 gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg
     9541 tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt gccacatcct
     9601 catctaaata tttaaatgag ataaatgtac ttttttatat aacttttagt tgttttctgc
     9661 ttaaaatcca tgccattttt ctattttcag cgaagaattc gaaccaggaa agcatgactc
     9721 gatactcgtc atatttcgcc ttactgcaaa tgcgcgatga agtatgacga gtatgaaaga
     9781 gtgatgcgga cacaaaggag taactatgtt tcatctcgat actttagcaa cgcttgttgc
     9841 cgcaacgctg acgttgctgc tcgggcgcaa gttggtccat tccgtctcct ttttgaagaa
     9901 atacaccata ccggaacctg ttgcgggtgg tttattagtg gcgctggcgc tgctgatact
     9961 gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc cgttgatgct
    10021 ggctttcttc gccaccattg gcctgaacgc caaccttgcc agtctgcgag ctggtgggcg
    10081 tgtggtgggg gttttcctga ttgtggttgt tggcctgttg gtgatgcaaa atgccattgg
    10141 cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgctgg ccggttctat
    10201 tacgctttcc ggcggtcacg gtacgggtgc tgcatggagt aaattgttca ttgaacgtta
    10261 tggtttcacc aacgcgacag aagtggcgat ggcctgtgca acgttcggtt tggtgctggg
    10321 cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca caccgaacgg
    10381 tattccggat gaccaggaag ttccgacggc gttcgaaaag ccggatgtgg gccgcatgat
    10441 cacctcgctg gtgctgattg aaactatcgc gctgattgct atctgcctga cggtggggaa
    10501 aattgttgcg caacttttgg ctggtactgc ttttgaacta ccgaccttcg tctgtgtact
    10561 atttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc gcgtctttga
    10621 gcgagcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat
    10681 ggggctgaaa ctgtgggagc tggcttcgct ggcgttgccg atgctggcga ttctggtggt
    10741 acagaccatc ttcatggcgt tgtacgccat cttcgttacc tggcgcatga tgggcaaaaa
    10801 ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac
    10861 ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca tggcgttcct
    10921 ggtcgtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg tgattaagct
    10981 gtatttgatg ttgccgattt ttgccggtta accgatgaag cggcggtctt ctcaccgccg
    11041 ctttttacgc attcgagtaa cgttctgttt ctggcatcca gcgttctatc agggcttttg
    11101 cctgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg
    11161 cctgatcgcg cagtaaatcc gccactttaa attcagcatt acccgtctga cgtgtaccta
    11221 acaattcgcc agggccgcga atctccagat ctttctgtgc aatcacaaaa ccatcgttgc
    11281 tgtcgcgtag tacctgcagg cggatttgcg cggttttgga gagcggcgtt ttgtagagca
    11341 gcacgcagtg agaagccacc gcgccacgac ctacacgccc gcgaagctgg tgtaactgcg
    11401 ccagacccag acgctccggg ttttcgataa tcatcagact ggcattgggt acatcaacac
    11461 cgacttcaat gacagtagtg gcaaccagca ggtgtagctc gccttgttta aacgatgtca
    11521 tcaccgcctg tttctcggcg ggtttcatcc gtccgtgtac caggccaaca ttcaactctg
    11581 gtagcgccag tttcaactct tcccaggtcg cttccgccgc ctgcgcttcc agtaattccg
    11641 actcttcaat caacgtacaa acccagtacg cctgacgccc ttcagtcata caggcgtggc
    11701 gcacgcggtc aatgatgtcg gtacggcggg tatcaggaat agcgaccgta gtcactggcg
    11761 tgcggcctgg tggcagctca tctatcaccg aggtatcgag atcggcatac gcagtcatcg
    11821 ccagcgtgcg ggggatgggc gtggcggtca tgatcaactg atgcggatgg aagccctgct
    11881 gctggccttt ctcccataac gccagacgct gatgcacgcc aaaacgatgc tgttcgtcga
    11941 taatcaccag cgccaggccg ttaaactgca cctgttcctg gaaaatggcg tgcgtaccaa
    12001 caatcatctg cacctgaccg ctggcgatgg cttcttgctg agccagtcgt gctttacctt
    12061 tctgttttcc ggcgagccag cctacttcga taccgagcgg tgcaaaccag ttacggaagt
    12121 tattggcgtg ctgctcggcg agtaattcgg ttggtgccat caatgccacc tgtttgccgt
    12181 gcgcaatcgc acgcaacgcg gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc
    12241 cctgcaccag acgcatcatc ggcacatcca gcgccatatc gcgctcgatc tccgccacta
    12301 cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggcggc gaggagttta tttttcagcg
    12361 cgtcattggc gctcagcggc tgagcatgaa aacgttgtgc accggcacgt aacgccaaca
    12421 tgctgaggtt gtgcgccagc aactcttcca gaatcagccg gcgttgtgcc ggatgctgcc
    12481 cggtttcgag atcgctaagc tgtagcgtcg gcggtgggcg gtgcaaagtg cgcaacgctt
    12541 ccggtagcgt catcattcct tgtgacagtt ccggcggcag gagttcttca atggcgcatg
    12601 tgtcgagcag atccagcgcc tggtcggtta atttacgcag cgtagcctgc tttacgcctt
    12661 ccgtagttgg gtaaaccggc gtgagcgttt cctgtaactc tggcgtgctg agatcgccct
    12721 gtacgcggta ttccgggtgg atcatctccg caccatattt gccgcgcttt gcttcgccat
    12781 aggccagcac gcggcggcct gtcgccaggc tatttttcat tgccgcattg aagttgaaaa
    12841 agcgcatagt gagaatgccg gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc
    12901 cgaaggagat attgcagttc agcacttcgc cttccaccgt ggcataaaca cccggcagta
    12961 gttcaccgat ggggtagaga tgtgtgcgat cttcgtagcg cagaggaagg tgtaagagca
    13021 aatcctgtac ggtatgcagg ttgattttcg ccagcttgtt actaagcgct gcgccaacgc
    13081 ccgttaggga actgagcggg acagcatcta acaggcgacc tttcatcgcg cttaccctgc
    13141 ggcctgcatg gtggaccacc agtcagcatc ggcttcaatc tcgccttgct gattaacgtg
    13201 ggggtagggc agacctttgc gttttgcgac tttcgccagc accggatagc cgccttcaaa
    13261 cagcaggcgt tgttgctcgg cttccggcaa catgctgttt tcacgcaggt acatgcccgc
    13321 attttgccgc tggcgctggg cttcgtaaag aatgagggct gaggcaacgg aaacattcag
    13381 cgactgcacc atgccgatca tcggaatgat gatatcctga tccgccaggg ccaacgcttc
    13441 ctgcgtgatg cccgttttct cctgtcccat caaaatacag gtcgggcgag tgtaatcaat
    13501 ttcgcggaaa tcaacagcgt tatcagaaag atgggttgcc agaatctgca tgccctggcc
    13561 tttcagatga gcgacggcat cgccaatggt gcggtgcgtt ttcacctgta cccagctgtt
    13621 actacccgca gctgccgaag ccatggtgcg catccggctg ccaggccaga cagcatgaac
    13681 ttcatgtacg ccaacggcat ctgcggtacg aataatcgca gaaacgttat gaggtttgtg
    13741 gacctgctcc atgcagacgg tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg
    13801 tgcataacgt gttgggttca taaaacatta atttcggttt cgggtgactt taatcacgtc
    13861 tggcatcacg cggattttgc gcatgatatt cgccagatgc acacggtcac gggcggtcag
    13921 acgaataaag gcgctataga cgcgaccatc tttctcttcc gtattcaaac tttgaatatt
    13981 cgaggtcgtg gtgttaattg ccgccgtcag gtttgccagc gcgccctgat gattgaacat
    14041 ctccaccttg atttcggtga tgaattcctg cgccgtctct ttatcccatt caacagccat
    14101 aaacttctct ggctctttct ggtagccacg gatgttacgg caggattcat ggtggatcac
    14161 cagaccttta ccggggctga cgtgggcgat aatcgggtcg ccaggaattg ggcggcagca
    14221 tttcgcaaag gtgatcagca cgccatcggc acctttaatg ggcagatgtc cgtgactttg
    14281 ggttgccggt ggaatggagg cgtccccatg ttgcagattt ttcgcgacca ccacgctcat
    14341 tgcgttacca aggccgattt ctgccagcag atcgtcaagc gttgccagct tcatgcggtc
    14401 cagctcgcgc tgaatatttt cctgcgggat ttcattgagc ttacggctac cacccaacgc
    14461 atggttgagc agacgacggc ccaggcttac agaatcatca cgcttgaggt ttttcagcaa
    14521 ctgacgaatt ttggcgcgcg ctttcgagct gacgacaaag ttcagccacg cggcattcgg
    14581 gcgagcaccc ggtgcggtaa tgatttcaac ggtttgaccg ctagtaagcg gctgcgacag
    14641 cgggtaaggc tggcggtcga cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac
    14701 cgcataagcg aagtcgacgg gcgttgcacc ggcaggcagt tcgacaatgc gcccttctgg
    14761 tgtgaaaacg taaatctcat ccgggaagag atcggattta acgctctcga taaattcaaa
    14821 cgagctaccg gcactctgtt gcagctccag caggctttgc atccagcgct gggcgcgtat
    14881 ttgcgcggta gtgctggttt cgccgtgctc tttataagcc cagtgcgcgg caacacccat
    14941 ctccgccatc tgatccatat cttcggtacg gatctggacc tcaaccggca cgccgtgcgg
    15001 gccaatcatc gaggtgtgca aagactgata gccgttcgct tttggaatgg cgatatagtc
    15061 tttcacgcgg cccggacgcg gcttgtacag gctgtgcatc tggcccagca cgcgataaca
    15121 ggtgtcagaa tcattgacga tcacgcggaa agcgtagatg tccatgattg agtgaaaacg
    15181 ctgctctttg agcaccattt tgcagtaaat cgaataaaga tgcttttcgc gaccactgac
    15241 gcggcacggt attcccgctt cctgcaaacg cccttcgatt tcagaaagaa tcttctggat
    15301 catctcttta cggttgccgc gcgcggcttt caccacttct ttgattacgc gataacggtt
    15361 gggatacagc gcctcaaaac ccagctcctc gagttcggtt ttaatgtggt ggatacctaa
    15421 acggtgcgcc agcgggctgt atatttcgag agtttcacgg gcgatgcggc gacgtttgtc
    15481 cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt tgatgagaat
    15541 gacgcggata tcctgcacca tcgccataat catcttgcga aagttttcgg cctgcgcctc
    15601 tttcttatcg cggaacttga gtttatcaag tttcgacacc ccctctacca gctcggcgac
    15661 gcttttacca aaaagctgtt ccatgtcctg gtaggtggcg ggtgtatctt caatcacatc
    15721 atgcagcagc gccgccatca gcgtttcata gtcgagtttc atctcggcca gaatgcaggc
    15781 aaccgccacc ggatgcgtga tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc
    15841 atcacgtgca acgagatacg cctgccgcag acgcttgatt tggtcttccg gcaggtagtt
    15901 ttgaatcagc tgattcaggc tttcaaacag atacaagggc gacccgcttt gtgattaacg
    15961 acgaccttca gcaatagcgg taacggcttg taattcagcg gcttcctgct cttgctgctc
    16021 ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga ccttcttcga tttcgcgcag
    16081 cgcgattaca gtggttttat cgttttcttc cggtaccagc ggatcctttc cgcctacctg
    16141 catctgacga gcgcgacgcg cggcgaccag taccaggtca aaacggttac caattttctc
    16201 tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag ctccacaggt gaagaaatga
    16261 ctgggcatga tactgaaatc aggttcagtc tgccaacaat ttgctgatta aagcgtcatg
    16321 acgctgcttt tggcggctca tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt
    16381 caacgcggta tcgaagtcat cattcacaat caggtaatca tattcggcgt aatggctcat
    16441 ttctgcaaca gcttgcgcca tacgctttgc aatgacctct tcgctgtcct gaccgcgacc
    16501 gcgtagacgg cggtccagtt cgattttaga cggcggtaaa ataaagatac tccgcgcgtg
    16561 cggcatcttc tggcgaattt gctgcgcacc ctgccagtcg atatcgagaa aaacatcgac
    16621 accggtcgcc agtacttgct caatggcttc acgcgaagtg ccatagtaat taccaaaaac
    16681 ttctgcgtgt tcgaggaacg catctctact aatcatttct ttaaattcat catgattaac
    16741 aaagaaataa tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac
    16801 agaaacctgg gtgtcataca acggttgggt ttttaataaa gcctgaatca ggctggattt
    16861 acccgcgcca ctgggggcag aaacaatata aagcgtgcct tgagccatga gtatctttcg
    16921 tatgtgatta gcgaaataaa gcctacatac gagcttatta tacacggcgc ggccatgtga
    16981 cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc tgcttccgca
    17041 tttgcctgtg attcactgca ataaagacga ttctcaggaa gcgtcctcgc agactccgtt
    17101 tttgccactc ggctttgcct tcaccacatt gtatatctgc ctgactatct acagcggagg
    17161 tgatgatgaa agtatggatg gcgatattaa taagtatctt gtgctggcaa tcatcggtgt
    17221 gggcggtctg tccggcctgg tcgccagcca gagcacagga agaaatttcc cgcctgcaac
    17281 agcaaataaa acagtgggac gatgactact ggaaggaagg gaaaagtgag gtggaagacg
    17341 gtgtttacga tcagttaagc gcccgtctta cgcagtggca acgctgtttt gggaacgaga
    17401 cccgcgatgt gatgataccg cctttaaacg gtgcggttat gcatccggtt gcccatacgg
    17461 gtgtacgcaa aatggcggat aaaaacgcat taagtctttg gatgcgcgaa cgtagcgatc
    17521 tttgggtgca gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg gacgggaaac
    17581 tgaacaaagc aatcagtcgc ggcaatggcc tgaaaggcga ggactggacg cagaaagttc
    17641 gcttaatttc cgctgtgccg caaaccgtta gcgggccttt agccaacagc acgcttcagg
    17701 gggaaatatt tctcaagcgt aaggggcata tccaacaaca aatgggggga ataaatgccc
    17761 gcgcaaaagt tgctggcctg atgatgcgcc aggggaatag cgatacgcta aactcactgg
    17821 ctgtttttgt ctgggcatgg ccggatggac cgcatttaat gaccgatcgt ttaaaggatc
    17881 tggctaccgc aggttttact ttaacgcaga cgtatacccg cgcggttaaa aatgctgatg
    17941 aggttgcgca cgtacgcaat gagtggtgga aagcaaaatt acccttcgtc accgatggcg
    18001 tggttgtgcg agcggcgaaa gaacccgaat cccgtcattg gctaccgggc caggcggagt
    18061 ggctggtggc ctggaaatat caacctgtag ctcaggttgc cgaagtgaag gcgattcagt
    18121 ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc acttgtacct gtcatgctgg
    18181 atgataaaaa agtccagcgg gtgaatattg gttccgtcag gcgctggcag gagtgggata
    18241 ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca ggggattcct cgcattgatg
    18301 atgtggtctg gcgcggagca gaacgtacaa aaccgacacc accagaaaac cgctttaact
    18361 cgttgacctg ctactttgct tctgatgttt gtcaggaaca gttcatttca cgcttagtct
    18421 ggctggggtc aaaacaggtt ctggggcttg atggcattgg tgaggccggc tggcgcgccc
    18481 tgcatcagac tcatcgcttt gagcatatct tttcctggct tttattaacg ccagagcaat
    18541 tacagaacac gccggggatc gcgaaaagta aaagtgcgca gttatggcat cagtttaatc
    18601 tggctcgtca gcagcctttt actcgctggg tgatggcaat gggaataccg ctaactcggg
    18661 cggcgcttaa tgccagtgat gaacggtcct ggtcgcaact tttatttagc acggaacagt
    18721 tctggcagca gctgccgggg actggatcgg ggcgcgccag acaggttatt gaatggaagg
    18781 aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc ccagcagatc acaggttttg
    18841 aaccttagtg accttcgtgg ctgtagtaaa agaccggtaa tcccagctta agacgtagcg
    18901 ccagtaaacg ggcaaaaaag ccgaatacca gggtggagat aatcaccaca tcatggttag
    18961 aaacatagtg ttgcagcgcg atgtacagca cggcggaggc aaatgagaca cccgcataca
    19021 actctttctg gaataccagt gggatgcgtt tacagaacat atcgcgcaaa acgccgccaa
    19081 acacgccggt ggttaccgcc gcgacaacgg caataatcgg gccgtgcccc atatccagag
    19141 caacctgtgc gccgatgata gaaaatacca ccagtccgag cgcatcgagc accagaaaca
    19201 ctttgcgcag ataaggcatt acaggagcaa ctatagtggt gagtactgca gcggtagcga
    19261 cgataatcac atattccggg tgtttgaccc agccgagcgg atagtggccc agcagaatat
    19321 cgcgcactga ccctccgcca attgccgtgg cggtcgcaat aataattacg ccaaatgtat
    19381 ccatgcgccg tcgtccggcc gccagcgccc ccgtcatggc ttcggcagta atgccaacca
    19441 aatacaaaat gtgtaacagc atggttctct ccctaaaaat ctgtctggaa gggtagcgat
    19501 ttgtgcgcat tgtcacgatt gagatttttt aaggagagaa tagattgata atttgtagtt
    19561 atgtgaaatt gctattttct gtagtaagaa tgcatttttc tgattttgtg ataagtaaaa
    19621 tgcattagaa aaagtatgga gtaaataaat atgaggacga aactatatgc gtagcgccgc
    19681 tgtaattaac aacggcctac ggagatatgt cgcttactcg atattctgaa tctgctcgcg
    19741 catctgttca atcaacactt tcagctcgat ggcggagttt gtcacttcgg cgttgataga
    19801 tttcgacgca agagtgttcg actcgcggtt gaactcctgc atcataaagt ccagacgacg
    19861 accaacggct tcttttttct tcagaatgtt gtaggtctct ttgacatgcg cttcgaggcg
    19921 gtccagttct tcggcaacgt caattcgttg tgccagcaga accaactcct gctccagacg
    19981 gttgttttcc agttgcacct gagcatcttc cagcttcgct accagacgct cacgctgcca
    20041 ttgcaggatt tccggcatat gggcgcggac tttgaccact tcggcggtga cgccttccag
    20101 acgctgctca atcaacactt tcagcgcctg accttcggtt tcgcgcgcga tgataaagtc
    20161 atccagggtg ccatccaacg ccgcgagaat ttcagctgca atcgcgtcga gatcttgctc
    20221 ctgggctgcc atcacgcccg gccagcgtag aatatcaacc gggttgattt ctccttcgtc
    20281 gctctgcatt tttacccagt tcgcggcagt taccagctgt ttagccagtt tttcgttgag
    20341 gatcagctca ccttgcgcgc taacatctgg ctcatagcgc agagtacatt ccactttacc
    20401 gcgcgtcagg cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac ggaactgctc
    20461 cggcagacga aagtaagttt ccagataacg ctggtttacc gagcgcattt cccaggttgc
    20521 gctcccccat tcacccttga tttcacgccg ggcgtaggcg gtcatactgc ggatcataga
    20581 cgttcctgtt tataaaagga gaggtggaag gattatagcc atcgatgcct tgtcaggata
    20641 ggaataaccg ccggaagtcc gtataatgcg cagccacatt tgtttcaagc cggagatttc
    20701 aatatgcgtc cagcaggccg tagcaataat caggtgcgtc ccgttaccct gactcgtaac
    20761 tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa agtattgtgt
    20821 accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc
    20881 accgcagagt acggtatgct gccgcgttct acccataccc gtaacgcgcg tgaagcggcg
    20941 aaaggtaagc agggtggacg cacaatggaa atccagcgtc taatcgcccg tgctcttcgc
    21001 gcggcagtag atttgaaagc actgggtgag ttcaccatta cgctggactg tgacgtgctt
    21061 caggctgatg gtggcacgcg taccgcttcg attacgggtg cctgcgtggc gctggcagat
    21121 gcgctacaga agctggtgga aaacggcaag ctgaaaacta atccaatgaa agggatggta
    21181 gccgcggtct ctgtcgggat tgtgaacggt gaagcggttt gcgatctgga atatgttgaa
    21241 gactctgccg cagagaccga catgaacgta gtgatgaccg aagacgggcg tatcattgaa
    21301 gtgcagggga cggcagaagg cgaaccgttc acccacgaag agttactcac cttgttggcg
    21361 ctggcccgag ggggaatcga atccattgta gcgacgcaga aggcggcgct ggcaaactga
    21421 tttttaaggc gactgatgag tcgccttttt tttgtctgta gaaaagtaag atgaggagcg
    21481 aaggcatgaa accatatcag cgccagttta ttgaatttgc gcttagcaag caggtgttaa
    21541 agtttggcga gtttacgctg aaatccgggc gcaaaagccc ctatttcttc aacgccgggc
    21601 tgtttaatac cgggcgcgat ctggcactgt taggccgttt ttacgctgaa gcattggtgg
    21661 attccggcat tgagttcgat ctgctgtttg gccctgctta caaagggatc ccgattgcca
    21721 ccactaccgc cgtggcgctg gcggagcatc atgaccttga cctaccgtac tgctttaacc
    21781 gcaaagaggc aaaagaccac ggtgaaggcg gtaatctggt tggtagcgcg ttacaaggac
    21841 gcgtaatgct ggtagatgat gtgatcaccg ccggaacggc gattcgcgag tcgatggaaa
    21901 ttattcaggc caatggcgcg acgcttgcag gcgtgttgat ttcgcttgat cgtcaggaac
    21961 gcgggcgcgg cgaaatttcg gcgattcagg aagttgagcg tgattacaac tgcaaggtga
    22021 tctctatcat caccctgaaa gacctgattg cttacctgga agagaagcca gaaatggcgg
    22081 aacatctggc ggcggttaag gcctatcgcg aagagtttgg cgtttaacga aactcgccgg
    22141 atgaaaagtc atccggcgtc atattactgc aactgtgcgg caattagcgg ccagcgggcg
    22201 tcaaaatcat ccgtcgggcg gtatttaaac tcgctgcgga caaatcgtga cagcatacct
    22261 tcacaaaatg ccagcaactg gcttgccagc agggtttcat cggtggtgta accttcaccc
    22321 tcacgcattc tcttttcacg caatacctga cgcaactgcg cttcaatacg ctcgaacagc
    22381 tggttgatgc gcccttgcag acgatcctgt tcgaacatta gcgcatgacc agtgaggatg
    22441 cgagtcaggc caggattacg ctcaccaaaa ccgaggagca acaacacgat cagacgcagg
    22501 cgcgctgtgg tgtctttctc atctttcaga atcaggttga tacgagtaat caggctatct
    22561 tcgataaact caatcaggct atcgaacatg cgggtcttac tggggaagtg gcgatacagt
    22621 gccgcttcgg aaacgccgac agaggcggcc agttttgccg tcgtgatacg ttggcttcca
    22681 tcgctggatt ccagcatcag cgccagagac tgaagtattt cctcgcgacg gttccttttc
    22741 gcagtttgtt tttctgccat gttacaaaat acccctgaaa ataagcactt gccaggcggc
    22801 acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg
    22861 tatgtgttac tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa
    22921 atcttccacc agattaaatt cagcctgtac taccggaaca aaaatcatct gggcgatgcg
    22981 ttcgccaggt tgaatggtga agctgtcctg accacggttc cacacggaaa tcatcaactg
    23041 gccctgatag tcagaatcga tcaatcctac caggttacca agcacgatac cgtgcttatg
    23101 tcccaatccg gagcgcggta gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc
    23161 cagcccggtc ggaaccagcg tagtgtcacc cggagccagt tctacggcgt cgtcgagaca
    23221 ggcacgcagg tcaagtccgg cagagccaga ggtggcataa gtcgggagcg gaaattcctt
    23281 cccaacgcgc gggtccagaa tcttaacgtc gatttttttc atcataacgg gtcacgatct
    23341 cgtcgagtaa taattggcca aggagctctt tgcgctcaag cggtaagact ttatctccgt
    23401 cctgccagaa aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa
    23461 catcgttcgc gcagatcaga tcaaggtttt tacggatacg tttttgccgg gcgtattctt
    23521 ccacattatt tgtttcggcg gcaaatccaa cgacgtaagg tcgatggtct tttagtgcgg
    23581 caacgcctgc gacgatatcg gggtttttaa ccatttttat tgttaattca tcaccctgcg
    23641 tggcctgctt tttgattttt tctggggcca cggtagctgc gcgataatcc gccacggcgg
    23701 cgcaaccgat aaaaatattt tgctgctgta ctgaagcatt cacggcggct tccatttcca
    23761 gcgcggtcat cacatcaaca cggttaacaa acggtggcgt cggtagtgaa accggacctg
    23821 ataccagcgt gacgttcgcg ccacgacggg ctgcggcggc ggcgatagca aaacccatct
    23881 tgccggagct gtgattagag atataacgca cgggatcgag cggttcacgc gtcggaccgg
    23941 cggtaatcat aatgttcaga tgtttcaggt cgttgacggg cgaaaaatgc gctaccgcca
    24001 tatccacaat agttaacgga tcgagcattc gtcctgggcc gatatcacca caagcctgac
    24061 tgccgctgtc tggcccccag atgagcaaac cacgtgaggc aagcacctct aaattatgct
    24121 gggtggcggc ggcacggtac atctgctggt tcatggcggg gagcacggct acaggcgcag
    24181 gtgtagccag acaaatcgtc gataccaggt cattcgccat tccggctgca acacgggcaa
    24241 ttaaatctgc cgtggcaggg gcgagaatca ctaaatcagc ccattttccc agctcaatat
    24301 ggcccatagc ggcttcggct gccgggtcca gcagactgtc ggaaacggga taaccagaaa
    24361 ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc ttcggtcatg gctacgcaga
    24421 catcggcccc gcgatcgcgc aaacgacgca ccagttcagg ggttttatag gcagcaatac
    24481 cgccgctaac gccgagaacg atttttttac cggccaggct catgatgatt tttcctgttg
    24541 ggtgacacca gaagttggcg attttatcac aatacttttg ttgtcgtgcc ttcacctgag
    24601 attcactttg cgaggcgctt tccaggattg aaaactggcc gtcgatttaa aggaacggct
    24661 atgacaggat gcgagcacca caaaggaggt gaaggtgaaa aacaatgtac agctgttgat
    24721 gccgcgcgaa aaaatgctga agtttggtat tagcgcctta acggatgtcg agctgctggc
    24781 gctatttctg cgtaccggaa cgcgcggtaa agatgtatta accctggcaa aagagatgct
    24841 ggagaatttc ggctctcttt atggcttgtt aacctctgaa tatgagcaat ttagtggcgt
    24901 tcatggaatt ggcgtggcga aattcgccca gttaaagggg attgccgaac tggcgcggcg
    24961 ttactacaac gtacggatgc gtgaagaaag ccctttactc agcccggaga tgacgaggga
    25021 atttttacaa agccagctca cgggcgagga gcgggagatc tttatggtga tctttctcga
    25081 ctcccaacac agggttataa cgcatagccg tcttttttcc ggcacgctaa accatgttga
    25141 agtccatcct cgggaaatta tccgcgaagc gataaaaata aacgcctcgg cgctgatcct
    25201 tgcgcataat cacccttcgg gttgtgctga acccagtaaa gcggataaac tcattactga
    25261 acggataata aagagttgtc agttcatgga tttacgcgtg ctcgaccata tcgtgattgg
    25321 gcgtggagag tatgtttctt ttgccgaacg cggctggatt taacccgcta tgcgcgatcc
    25381 ttcgggatct ttgtctgttc gggacttgag cacatcgctg agtcagcgta tactacgcca
    25441 cctttgagaa tctcgggttt ggcatttggg cctggcaatc gagagttcac atagaactgc
    25501 gatgaccggg ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct cgagctaatt
    25561 tgatttttgg agaatagaca tgtcccgagt ctgccaagtt actggcaagc gtccggtgac
    25621 cggtaacaac cgttcccacg cactgaacgc gactaaacgc cgtttcctgc cgaacctgca
    25681 ctctcaccgt ttctgggttg agagcgagaa gcgttttgtc accctgcgcg tatctgctaa
    25741 aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg
    25801 tggcgaaaag tactaagtac ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa
    25861 atcaagctgg tttcttctgc tggtactggt cacttctata ccactacgaa gaacaaacgt
    25921 actaagccgg aaaaactgga actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc
    25981 tacaaagaag cgaaaatcaa ataatttccg ctttaatgta acaaaaaacc tcgccccggc
    26041 ggggtttttt gttatctgct tgcccccata ttgactgcat ctgttcattc ctggagatgc
    26101 tatgcctgaa ttacccgaag ttgaaaccag ccgtcgcggc atagaaccgc atctcgttgg
    26161 tgcaaccatt cttcatgcgg tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga
    26221 gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag cgccgggcta aatatctgct
    26281 gctggagctg cctgagggct ggattatcat tcatttgggg atgtctggca gcctgcgcat
    26341 ccttccagaa gaacttcccc ctgaaaagca tgaccatgtg gatttggtga tgagcaacgg
    26401 caaagtgctg cgctacaccg atccgcgccg ctttggtgcc tggctatgga ccaaagagct
    26461 ggaagggcac aacgtgctgg cccatcttgg accggagccg cttagtgacg atttcaacgg
    26521 tgagtacctg catcagaagt gcgcgaagaa aaaaacggcg attaaaccgt ggctgatgga
    26581 taacaagctg gtggtaggcg tagggaatat ttatgccagc gaatcgctgt ttgcggcggg
    26641 gatccatccg gatcggctgg cgtcatcact gtcgctggcg gagtgtgaat tgttagctcg
    26701 ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggc ggtacaacgc tgaaagattt
    26761 tctgcaaagt gatggtaaac cgggctattt cgctcaggaa ttgcaggttt acgggcgaaa
    26821 aggtgagccg tgtcgggtgt gcggtacgcc gattgtggcg actaaacatg cgcagcgggc
    26881 aacgttttat tgtcggcagt gccagaagta attgatgcgc gccggatggc ataccatccg
    26941 gcataaactc tacgctaact tcgccatcag cgcctgatgg acattctccg gcaggaaatg
    27001 ggtgacatcg ccctgatggc gcgccacctc tttcaccaac gatgaagaga taaacgacca
    27061 ctctttcgac ggcatcagaa acacactttc cagctccggc attaagtggc gattcatatg
    27121 cgccagctgc atttcatatt caaaatccgc caccgcacgc aggccacgaa tcagcaccgt
    27181 agcgtgttga ttacgggcga agttcgccat caaatcacta aacccgacca cttccacgtt
    27241 ctccagatgc gcggttgcct gctgtgccag ctccacacgc tcttccaggg taaacatcgg
    27301 ttttttactg ggactagcgg caatcgccag aataacgtga tcgaacattt gcgaggcgcg
    27361 cgtcacgata tcgatatgac cattggtaat gggatcgaaa gtacccggat aaatcgcccg
    27421 tttttgcata acaacctcaa tgcgttttcg gtggcaggta aggttccagc agttgaagca
    27481 gacgctgtag tgcgccctgg ttttgataca gcacttcaac ggcatgacgg ccatagaaac
    27541 tacggtaatc ggcgtcggtg agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg
    27601 taacggtaat cagcccgctt gcctgctcca gccgcgcgca aatgtcttta aagttaaacg
    27661 tatgcggccc catcaatacc ggaatagcgt gtgcggccgc ttccagcgga ttatgcccac
    27721 cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac agtaacatca
    27781 actcgcccat cgtatcgcca accacaacct gcgtgctggt ggaggggact tcccctgaag
    27841 atcgtgtgat atagcttagt ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt
    27901 ctggatgacg gggtaccagg atgagcaata aattcgggaa ttgctgtaac aatgcctgat
    27961 gtgcggcgat caccacactc tcttcgcctt catgagtgct ggtggcaatc cataccgggc
    28021 ggtgtggtgc ccactggcgg cgcagcgtca cggctttagc agccaactgc ggcgttacag
    28081 aaatatcgaa tttcaggcta ccggtaacgg tcacctgatt attttttgcg cccagcgcca
    28141 caaaacgtgc accatcttct tcattttggg cggcaatcag cgtaatacga cgcagcaagc
    28201 ggcggacgaa tttacccagt ttggcataac ctgcggccga gcgggcagag agtcgcgcgt
    28261 tagcgatcac cagcggaatt ttacgtttat gtagcgccgc aatcaggtta ggccatagtt
    28321 cggtttccat aatcaacacc agtttagggt cgactttatt caggaaacgg ttgagtgcat
    28381 cgggcaggtc atacggcaga taaacgtgct gaacatcctt cccgaaagcc gattgtacgc
    28441 gctccgaacc ggttggagtc atggttgtta cggtaatcgg taaatcagga taacgatgac
    28501 gcagtgcgcg caccaacggg atcgccgcca gagtttcacc gacggagacg gagtgcagca
    28561 taatgccgcc tggttttagc ggatggcggt aaaaaccgta acgttcaccc cagcgttttc
    28621 gataggccgg agccttacgt ccgcgcaccc agagccgtat ccagatcagt ggctgaataa
    28681 ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa tagctgactt atggatgtgc
    28741 tggggattct atgtatttag ctgtggcttt accattactt ttcccgtttt tgacttaaat
    28801 agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt ttatgcgatt
    28861 cattgatttc tgcagtacgt gcaggaagga atcgcagagc aacagtaaaa agaatatggt
    28921 tgctaatgat tttatttgat tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca
    28981 ttcgggtcgg attgccgcga aagaagtgat acactagcgc gtcaatatca gtgcggtatc
    29041 agtattcagg tagctgttga gcctggggcg gtagcgtgct ttttctgctt caccttaatc
    29101 ggataatctc aacaaaaaga gttacttgtg gataagccat ttcgaagaat tttgctcatt
    29161 aagatgcgtt ttcatgggga tatgttatta actactcccg tcattagttc gctgaaaaaa
    29221 aattaccctg acgcaaaaat cgatgtgctg ctttatcagg acaccatccc gatcctgtct
    29281 gaaaatccag agattaacgc gctctacggc ataaaaaata aaaaagcaaa agcctcagaa
    29341 aaaattgcca acttttttca tctcatcaag gtattacgtg ccaataagta tgaccttatc
    29401 gttaatctta ccgatcaatg gatggttgct atactggttc gcttattaaa tgcccgtgtg
    29461 aaaatttccc aggattatca tcatcggcag tctgcttttt ggcgtaatag tttcactcat
    29521 ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct gacgccattg
    29581 ggacttgaat cgttggtgaa gcagacaacc atgagttacc cgcctgcaaa ctggaaacgt
    29641 atgcgtcgcg aacttgatca cgctggtgtt ggacaaaatt atgtggttat ccaacctacg
    29701 gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat tgatgcctta
    29761 catgctcgtg gttatgaagt cgttctgacg tccggcccag ataaagacga tctggcctgc
    29821 gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc cggaaaggtg
    29881 accttcccgg aacttggtgc gttaatcgat catgcgcagc tgtttattgg cgttgattcc
    29941 gcaccggcgc atattgccgc tgcagttaat acgccgctga tatcgctgtt tggtgcgacg
    30001 gaccatattt tctggcgtcc ctggtcaaat aacatgattc aattctgggc gggagattac
    30061 cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc ggttattccg
    30121 gcggcagatg tcattgcagc tgtcgataaa ttacttccct cctccacgac aggtacgtcg
    30181 ttatgatcgt tgctttttgt ttatataaat attttccctt tggcggtttg cagcgcgatt
    30241 ttatgcgtat tgctcagaca gtcgccgccc gaggccatca tgttcgggtt tatacccagt
    30301 cgtgggaagg cgaatgccct gatgtatttg aactgatcaa agtgccggtt aaatcgcata
    30361 ccaatcacgg gcgcaatgcg gaatattttg cctgggtgca aaaacattta cgtgaacacc
    30421 ccgtcgatag agttgttgga tttaataaaa tgccggggct ggacgtttat tatgccgctg
    30481 atgtttgtta tgccgagaaa gtagcgcagg agaaaggctt tttctatcgc ctgacgtcac
    30541 gttatcgcca ttatgctgcc tttgagcggg caaccttcga acagggcaag ccgacacagc
    30601 tgctgatgct gacagataag caaatcgccg atttccagaa acattatcag actgaagcgg
    30661 aacgttttca tattctgcca ccggggattt atcctgatcg taaatatagc cagcagccag
    30721 ccaatagccg tgaaatcttc cgtaagaaga atggaataac cgaacaacaa tatttattgt
    30781 tgcaggttgg ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt
    30841 cgttaccgga ttcgctgcgc cacaacacat tgctatatgt tgttgggcag gataaaccgc
    30901 gaaaatttga ggcgctggca gaaaaacgcg gcgtgcgcag taatgttcac ttcttctcgg
    30961 ggcgcaacga tgtctcggaa ttaatggcgg cggcggattt attacttcat cctgcctacc
    31021 aggaagccgc gggaattgta ctgctggaag cgataactgc aggattaccg gtactaacaa
    31081 cagccgtttg tggctatgcg cattatattg ttgacgccaa ttgcggcgag gctattgctg
    31141 agccattccg ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg caatcttcat
    31201 tgcgccaggc ctgggcggaa aatgcgcgac attatgctga tacacaagat ttatacagtc
    31261 tgccagagaa agcggcggac atcataacgg gtggtctgga tggttgaact taaagagccg
    31321 tttgccacgt tatggcgcgg taaagatcct tttgaggaag ttaaaacctt gcagggtgag
    31381 gtatttcgtg aactggaaac tcgccgcact ctgcgctttg aaatggcggg caaaagctat
    31441 tttctcaaat ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt actctcattg
    31501 cggatgccag tattaggcgc agaccgcgaa tggaatgcga ttcatcgact gcgggatgtc
    31561 ggcgttgata ctatgtatgg ggtggcattc ggcgaaaaag gcattaatcc gctgaccaga
    31621 acctcgttta ttattaccga agatctgaca ccaaccatca gtctggaaga ttactgtgct
    31681 gactgggcga ccaacccacc agatgttcgc gtgaagcgta tgcttattaa gcgtgtcgcg
    31741 acgatggtgc gcgatatgca tgctgcgggc attaaccacc gtgactgtta tatctgtcat
    31801 ttcctgctgc acttgccttt ttccggtaag gaagaggagt taaaaatttc agtaattgac
    31861 ctgcaccggg cgcagatacg tgcaaaagta ccgcgtcgtt ggcgggataa agatttgatt
    31921 ggtttgtatt tttcatcaat gaatattggg ctgacgcaga gagatatctg gcgatttatg
    31981 aaggtttatt ttgggatgcc tttacgtaag atattatctt ttgaacggaa tttattgaac
    32041 atggcaagtg ttaaggccga gcata
//
LOCUS       DACADA010000039        32004 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.039, whole genome shotgun sequence.
ACCESSION   DACADA010000039 DACADA010000000
VERSION     DACADA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.039"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(174..779)
                     /locus_tag="ISB17_001787"
     CDS             complement(174..779)
                     /locus_tag="ISB17_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979728.1"
                     /translation="MPSDDDFNSRFKQEVARRLKDSLNLHRQLAEEVEPEDDETPVQS
                     PDEASPQPKVSHAPKKTAVRIRGIARKRAAEARKMAVAASSKRIPTATPSQQVTRTES
                     KILTEFTSVKQVNSLDTEHERKQLNNKDFEAEIALKKRYGKWFLIILAFQLLIMNGVF
                     IADGAGKLEFEDLTLQLYMGGTLTEVFGLVLVVTKYLFKRK"
     gene            complement(789..1277)
                     /locus_tag="ISB17_001788"
     CDS             complement(789..1277)
                     /locus_tag="ISB17_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP1979729.1"
                     /translation="MATNPREHARRVLDTFWCGRGFPVDPAKIAHDMGLDVFITDLPG
                     KVSGALIKQKDQDPAIFLNSDDNKVRQRFSCGHELGHYIARQASHSDEYEYVDLRGET
                     ASAGTHPDEIFANKFAAELLMPVDEVRKLHADGQPSYIMAHYFGVSDDAMTYRLKNLR
                     LG"
     gene            complement(1676..1909)
                     /locus_tag="ISB17_001789"
     CDS             complement(1676..1909)
                     /locus_tag="ISB17_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979730.1"
                     /translation="MGTLGNQPCRSRYEFDDFNLANRAAALTKLAKETGVSVDTLIKL
                     EIAMQLNRLNSILIENGDKMDENLAGIGEILQK"
     gene            2153..2794
                     /locus_tag="ISB17_001790"
     CDS             2153..2794
                     /locus_tag="ISB17_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979731.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTVVAGHPEIDVSELDIGPLYRYYKRGEKGNPLADLLIELAPPTCERVRISPEVY
                     IIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLIPIYRSDK"
     gene            2946..3125
                     /locus_tag="ISB17_001791"
     CDS             2946..3125
                     /locus_tag="ISB17_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979732.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
     gene            3203..3799
                     /locus_tag="ISB17_001792"
     CDS             3203..3799
                     /locus_tag="ISB17_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAP1979733.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPAERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGSISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            3796..4089
                     /locus_tag="ISB17_001793"
     CDS             3796..4089
                     /locus_tag="ISB17_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAP1979734.1"
                     /translation="MKKIDLRKAARSRACTVRIPGVCNHNPETSVLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEHDSLLLMHAEGVFRTLAIWRDEEFI"
     gene            4089..4760
                     /locus_tag="ISB17_001794"
     CDS             4089..4760
                     /locus_tag="ISB17_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1979735.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMSDFEFADSSWRRSVNSLDASQQ
                     AWLRYCYGGNLAFKHQTAICEAVWSRYKGNIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSTLAGMLSVSRSTWCETYSLHWVGMKEAVRALDEVALLATLHHYQNHL
                     DDVCV"
     gene            4873..5256
                     /locus_tag="ISB17_001795"
     CDS             4873..5256
                     /locus_tag="ISB17_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979736.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            5256..5528
                     /locus_tag="ISB17_001796"
     CDS             5256..5528
                     /locus_tag="ISB17_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP1979737.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRIFYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            5528..6007
                     /locus_tag="ISB17_001797"
     CDS             5528..6007
                     /locus_tag="ISB17_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HAP1979738.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERAGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADEVNIKAFPRSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            6015..6209
                     /locus_tag="ISB17_001798"
     CDS             6015..6209
                     /locus_tag="ISB17_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979739.1"
                     /translation="MKKLLLAVAFFTLAGCTHSTYTEATRADGSSIKHVMIAPGTKIT
                     TANGGCIDSTGAEASCLGGK"
     gene            complement(6269..6514)
                     /locus_tag="ISB17_001799"
     CDS             complement(6269..6514)
                     /locus_tag="ISB17_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979740.1"
                     /translation="MNREKIEALMSVILKEADPKKFPEKLSEDAIVSFYRANSSYLNC
                     STLREWLSGEAEPSDEILQKIMVQNNWRATKWSNQLP"
     gene            6883..7449
                     /locus_tag="ISB17_001800"
     CDS             6883..7449
                     /locus_tag="ISB17_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979741.1"
                     /translation="MASQAEVAAHLLLSDRRLRDLAKLPGAPVPQGRGDWELDAWRHF
                     YIRYLRSNRRDTTSNDEPEAGDNSPEKNREQWLKNEERQERILMARVKRRILAKRYAP
                     IELISVAVSRVAVELRTRVESWPPRLKKVWPEMPQEASSVLREELAIALNELSDIRID
                     FSDYDVSDIERDLDRVESLARDDTDDGC"
     gene            7436..9298
                     /locus_tag="ISB17_001801"
     CDS             7436..9298
                     /locus_tag="ISB17_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAP1979742.1"
                     /translation="MTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTNDAIKI
                     VSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAEMPVI
                     QKIFPDWDKSNERNNLQRKEMSGAILDFRGASAPGNFRRLTKQVVEGDEVDGWPLEVA
                     KKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYLTCPH
                     CGEEQVLVFGFDGIEYGLKWDNSLQTNEAKSSSAYYQCCHCPEHFYYRDLEKMEFGGR
                     WIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKAKGDP
                     LKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYITGGIDSQTSGRY
                     ECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSRWAWD
                     TGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTDTAKD
                     LLAMRLQLEPDSKSATPGAIHFPNDDEIFSTTEAKQLVSEVLIPKLINGRVVYRWDNQ
                     GRRNEALDCWVYGLAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS"
     gene            9298..9531
                     /locus_tag="ISB17_001802"
     CDS             9298..9531
                     /locus_tag="ISB17_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979743.1"
                     /translation="MTSREVLTERLLEAEIALHKLLTGKSTVSLSHGDSAGNNRSYQY
                     SQASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            9528..11102
                     /locus_tag="ISB17_001803"
     CDS             9528..11102
                     /locus_tag="ISB17_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1979744.1"
                     /translation="MTAPQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSAD
                     AALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYLGISREDA
                     RALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESSAG
                     SVFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCR
                     RIPAQLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMY
                     AATIESELDSQQAFEYIAGAGTDIDSNPLNSFIQSYVTYYNGANIKLGGVKVPHLHPG
                     DKLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWR
                     FFMGRRRFIAARQASLMFCCWFEEALARGIITLPRSAVRSFYEARNSWTNALWIGAGR
                     MAIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSW
                     SVSHPSNTNDNGWGGN"
     gene            11102..12409
                     /locus_tag="ISB17_001804"
     CDS             11102..12409
                     /locus_tag="ISB17_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAP1979745.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPTYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYNGIAKRLQQAISDPDVRGVLLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPGDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKMERFMSGTETTAETTTTEQTATTTTVAQVEFNTEQIR
                     ADAASSELARVMAIINCPEAVGREAQAKALAGVPGMTVGQAQVVLAAAPKTAQARTET
                     ALDTLMSTESPATIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            12409..12738
                     /locus_tag="ISB17_001805"
     CDS             12409..12738
                     /locus_tag="ISB17_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAP1979746.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATVG
                     TFKVWDGSVGKAIGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPISVG"
     gene            12797..13831
                     /locus_tag="ISB17_001806"
     CDS             12797..13831
                     /locus_tag="ISB17_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAP1979747.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYLDPVTDTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGISEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            13866..14285
                     /locus_tag="ISB17_001807"
     CDS             13866..14285
                     /locus_tag="ISB17_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP1979748.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNTASGTDNELAQSIASAIVSQSQEQELAPATRRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            14282..14662
                     /locus_tag="ISB17_001808"
     CDS             14282..14662
                     /locus_tag="ISB17_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979749.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIEPDGSVTWSGWTDYVVKEPQP
                     DGTGLTNIFLEPHTSSENSEYSKY"
     gene            14694..15374
                     /locus_tag="ISB17_001809"
     CDS             14694..15374
                     /locus_tag="ISB17_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979750.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMVYNRALNRTA
                     KHMHRISAGMILTALAAKNHKAVDKRIKPFIKRRNFTKEGAGDLSSAKLWYGLNDFRV
                     SELKGRLQNPRRQKQPRDLETGLFLKTKKGARGATFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLDRGGIEEARVPVHDALEDAIDDYIFENIGPVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            15371..15907
                     /locus_tag="ISB17_001810"
     CDS             15371..15907
                     /locus_tag="ISB17_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979751.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDESVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWINGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHEGDYYGPIGR"
     gene            15888..16790
                     /locus_tag="ISB17_001811"
     CDS             15888..16790
                     /locus_tag="ISB17_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1979752.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDDKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVQDRQGTITEV
                     RTTYNGHTHICPDGETQQPNQPMA"
     gene            16793..17134
                     /locus_tag="ISB17_001812"
     CDS             16793..17134
                     /locus_tag="ISB17_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1979753.1"
                     /translation="MLGMDRNTGKLLSGTDHIRQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            17131..18051
                     /locus_tag="ISB17_001813"
     CDS             17131..18051
                     /locus_tag="ISB17_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1979754.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKGSDLDQIGANFDCL
                     RLLITPANPDVIPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGTPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLAIYVDTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDVIAGVGQAPYCTEIKLSVQMG"
     gene            18054..18680
                     /locus_tag="ISB17_001814"
     CDS             18054..18680
                     /locus_tag="ISB17_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1979755.1"
                     /translation="MTSQSVLPPNAITPERALEVVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPRDDPYTFAIKIYLLKSMGLDLELLNTFIAQIFDAKNCRSLLKEINFETGVDG
                     EFFIAGTPYAKIGVNVPADGDGGVKINGGLYISGSPIVSLNVEIGPNG"
     gene            18673..19857
                     /locus_tag="ISB17_001815"
     CDS             18673..19857
                     /locus_tag="ISB17_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979756.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSAENSGNQVIFTLYVPPDTGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVVYDNYVTPEEADKKYLQISKNL
                     SEIPANGETAQQAARKNIGIDGDVAYRDKGNAFTHPNTFKDDVIFEDVLSSKKQININ
                     KPGTDLTLFSFAGLSDVSGDYLDFSTRGENNQKVFYRLRGVHYDGLVLLCGGQTYKYW
                     NEANLKPVKTINGESPDENGNIDISLSPDPSPEIGVVGAVGTYALVAINHAPQILPGS
                     IWAGGDLYYASINNTGSYNTDIATKPFYDTPLIGSWRAMAYSPGGVGDIGTACFLAIR
                     IA"
     gene            19857..20246
                     /locus_tag="ISB17_001816"
     CDS             19857..20246
                     /locus_tag="ISB17_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979757.1"
                     /translation="MISVDSFRKITNPVFNDEHGLSINIICDIDGIGNDIPFTAMKND
                     CEEHGRVLYERALSGDYGSISKFVKKEQTQKLQTTLRTVGDLSIEAFSIQCAVDAGTA
                     SDAQIENLAELKEAIATRIGENREGIK"
     gene            20243..21745
                     /locus_tag="ISB17_001817"
     CDS             20243..21745
                     /locus_tag="ISB17_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979758.1"
                     /translation="MSVTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSSYPTC
                     ASLLWGSELADNLVEFSTVMPGADGNNWIVEIVDLGINGNVATPGYSTLPDGSRKLTM
                     ITDGAMTPSKMYDQNQQYKEGLQIGEYINVTFGGDHAGTGTVFALPPTNLSGGKDESF
                     KYNVPTLIAGSQKKAGLLGGSGTLPPAVSEILNQEDAIIVVVRVEEDSDETKMRQNVV
                     NGINALLTSAQINQVTPRILIAPDYSANDYIAEQLEVVTNKLRGVGYIDSPRGATPAD
                     VVNRRQRYGGRMEILRPRVYSTSDVSGLSRPYSAIAAGLRARIDNEKGFWWSKSNQNI
                     YGITGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLSPQWSFECVR
                     RTADVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDEE
                     LNTKESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETV"
     gene            21763..22275
                     /locus_tag="ISB17_001818"
     CDS             21763..22275
                     /locus_tag="ISB17_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979759.1"
                     /translation="MADSNTYRAFALFVQGERVLNCTEYTPVDMKIIEDDFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMGYLGLIPGAKTRFEIRSAYTDNYGVNFERIDTY
                     EGLITAITDDAQGTDSKSAVGQSVTIAPSYYKRVQNGKIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            22288..22569
                     /locus_tag="ISB17_001819"
     CDS             22288..22569
                     /locus_tag="ISB17_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP1979760.1"
                     /translation="MESLLDSMTITLSRPFIIKGESRDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            22678..24318
                     /locus_tag="ISB17_001820"
     CDS             22678..24318
                     /locus_tag="ISB17_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979761.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLEKWTG
                     RAKGAAKWGGRAAAGAVRATGRGIKWGTLGAAGLIGGAAAGALAMNAETSEKLGLAKS
                     YGVGVEKYAAWENIGKAAGLNGENIGDLSEELTNKIGEIGNEKSLNPMLFQIGLTKKR
                     MAGWDREKQFNEVMRRISEMKDEKQAASLADQLMGGEANKIMTYMKATGKSWEQTMSD
                     AQKSNLLTKEGAEGAARAHVSVTNLWGSITSGLADTLGKIGGELAPTFDSVRETFTSW
                     FKDNQGGFVSTITEWVKPENMKKMWDGIVNFGEGCVKFGKIIWAVVKKLEWLIPDEKS
                     DEEQKTYNDEYNRAYQEFMDSGGKYSPNAGLTADNIAKAKAEEAVENMRHPERQEQAK
                     SQAESMLAFVNPLSGMVNKNTAPAALDSSTLNIDTLKQAVSTPAPEQNNKIEINIVGA
                     TDPQSIQQSAASGVLDGLRQAASSYNRGAMFDKPAAAG"
     gene            24354..24743
                     /locus_tag="ISB17_001821"
     CDS             24354..24743
                     /locus_tag="ISB17_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979762.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRFGKNDALQITGR
                     PNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVKGYWVLRELTET
                     QNSFLMGGTPKSQDFSLTLEYYGASLD"
     gene            24905..25129
                     /locus_tag="ISB17_001822"
     CDS             24905..25129
                     /locus_tag="ISB17_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1979763.1"
                     /translation="MGPQMITYTTRDGDRLDQICLAVYGRASKTTETVLYQVSNYGVT
                     DMCAVFRAGEKIVLPDIEPEPVKKETQLWD"
     gene            25133..26177
                     /locus_tag="ISB17_001823"
                     /pseudo
     CDS             25133..26177
                     /locus_tag="ISB17_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
     gene            26344..26763
                     /locus_tag="ISB17_001824"
     CDS             26344..26763
                     /locus_tag="ISB17_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979764.1"
                     /translation="MVVKNGLACGIMLALVGCATKPVSNEQATPVPEKQIISTALLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
     gene            27235..27900
                     /gene="yecA"
                     /locus_tag="ISB17_001825"
     CDS             27235..27900
                     /gene="yecA"
                     /locus_tag="ISB17_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAP1979765.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(27951..29162)
                     /gene="tyrP"
                     /locus_tag="ISB17_001826"
     CDS             complement(27951..29162)
                     /gene="tyrP"
                     /locus_tag="ISB17_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAP1979766.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            29353..29592
                     /locus_tag="ISB17_001827"
     CDS             29353..29592
                     /locus_tag="ISB17_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAP1979767.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            complement(29630..30127)
                     /gene="ftnA"
                     /locus_tag="ISB17_001828"
     CDS             complement(29630..30127)
                     /gene="ftnA"
                     /locus_tag="ISB17_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAP1979768.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30299..30622)
                     /locus_tag="ISB17_001829"
     CDS             complement(30299..30622)
                     /locus_tag="ISB17_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979769.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            30886..30972
                     /gene="azuC"
                     /locus_tag="ISB17_001830"
     CDS             30886..30972
                     /gene="azuC"
                     /locus_tag="ISB17_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAP1979770.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            31087..31338
                     /locus_tag="ISB17_001831"
     CDS             31087..31338
                     /locus_tag="ISB17_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAP1979771.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(31416..31919)
                     /locus_tag="ISB17_001832"
     CDS             complement(31416..31919)
                     /locus_tag="ISB17_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAP1979772.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT         8678 a         7270 c         8287 g         7769 t
ORIGIN      
        1 tcttggagaa ttattttttt aagggtaggt gttgacgtta gagcaaaaga atggtgattt
       61 ttttgatgtg tattacgttg gaactgatgg gaataactat ctgttttcaa cgcactgcta
      121 atttatcaag gccctttgat cagggccttc gaaagactgt tggaccgaga gagttacttg
      181 cgtttaaaaa ggtattttgt cacgaccagc acgaggccga atacctctgt caaagtaccg
      241 cccatgtaca actgtagtgt taaatcctca aattccagct tacctgcgcc gtcggcaata
      301 aatacaccat tcattatcag aagttggaaa gcaaggataa tcaggaacca tttcccgtat
      361 cgttttttta gagcgatttc tgcttcaaaa tctttgttgt tgagttgttt gcgctcatgc
      421 tcggtatcaa gtgaattcac ctgtttcaca gatgtgaact cggtcaagat tttactctca
      481 gtacgggtga cttgttgaga gggtgtagca gttggaattc ttttgctaga cgcagctaca
      541 gccattttgc gagcttcggc agctcttttt ctggctattc cgcgaatacg gacagccgtt
      601 tttttggggg cgtgcgaaac ctttggctga ggtgaagctt cgtcaggaga ctgaaccgga
      661 gtttcatcgt cctccggctc aacctcttct gctaactgac ggtgtaggtt gagggaatcc
      721 ttcagtctgc gtgctacttc ctgtttgaaa cggctgttaa aatcgtcatc cgatggcatt
      781 ttaaagcgtt accccaacct caagttcttt aaacgataag tcatcgcatc gtcagatacg
      841 ccaaaataat gcgccattat ataacttggt tgaccgtcag cgtgcagttt tctgacctca
      901 tctaccggca tcagaagttc tgctgcgaac ttgtttgcaa aaatttcatc aggatgcgtt
      961 ccggcgcttg cggtttcgcc acgtaaatca acgtactcat attcatcgct atgcgatgct
     1021 tgccgggcga tgtaatgccc caactcatgc ccacatgaaa aacgttgacg aactttgttg
     1081 tcatcactgt ttagaaagat cgctggatct tgatcttttt gtttaatcag tgcgccagaa
     1141 actttcccag gaagatcggt aataaaaaca tctaatccca tgtcgtgagc aattttcgct
     1201 ggatcaacag ggaaaccacg cccgcaccag aacgtgtcca gcactcgccg ggcgtgctct
     1261 cttgggttgg tagccatagc tcctccagtt actgcattac gattattggt tgaaatatag
     1321 acagcatgtt ccgtaccaca ttcctgtaaa agtgcaaatt ctgcacttct ggtacgatat
     1381 taactgttag tagtaaagtc ttccagataa atcactttaa aatcttgtga attgcttgct
     1441 ttttacgctg tattgtggat ggttcaatgt ttccagtaaa aacatacatg ataatcatga
     1501 gtttagtgtt tttttaggcg tctatatgtt tctggaaaat acaggtctaa cctgatctta
     1561 tcgttgagat tttttgtact cagtatgcgt gaacgattcg accaggtgac tgtgtacgtg
     1621 aagaagaccg cccttaatat tttaaatgtg caacccctca gaagaggggc tactctcact
     1681 tctgcagtat ctcgccaata cctgccagat tctcgtccat cttgtcaccg ttttcgataa
     1741 ggatggagtt gagccgattc agttgcatag caatttcaag tttgattaag gtatccaccg
     1801 atacaccagt ttcttttgct aacttagtaa gagccgcagc tcgattggct aaattaaaat
     1861 catcgaactc ataacgactt ctgcacggct ggttgcctaa cgtacccata gtgattcctc
     1921 atgatataaa gacccaacaa tataaattac caggcacatc ataagatagg gttccagccc
     1981 atattttcaa atatttgcaa gtatcaccgt tcagtctaaa aagttggatg atgatttaac
     2041 gagccataac aaaaccctgc ctcggaggtt ttttcttttt tatagaactc agcggcgtga
     2101 aaacgaataa taaattgtgc aaatagtgca gtttttatta ttcgaggtaa ttatgaaaac
     2161 cttctatgag gactggcctg aaactttcgt tagccggtta gatatgttac gggcgttgga
     2221 tgatcgcggt tcgacccgga ggctctatct tgaacggacc ggggcaatat ttgacgctct
     2281 ggcagaagag atacgtacag tcgttgccgg gcatcctgaa atcgacgtga gtgaacttga
     2341 tatcgggccg ctgtatcgct attacaaacg tggagaaaag gggaatccgc tggctgactt
     2401 acttattgag ttggctccac caacttgtga acgggttcgt atatctcctg aagtgtacat
     2461 aatcccgtat ctgttttttg cactgttgat agcgcaaggc gctgacaacg atgcccgcga
     2521 ttttttcaat atgatgatgc gaccgttaat catcgcctac cgtttcaaac aactggcgcg
     2581 atacctggga acaaagggcg gaggacgacc acagcacaga ttaaaaagcg aagccattga
     2641 actggctgat cgttttttta ctgaaaaccc gacagcgcca ttatcgcgtg gcgtgcaata
     2701 catatccggt atttttgtgg cgaaatactc tgacccacct gcagcgtcga cgattagaaa
     2761 atggttaatt ccaatttaca ggagtgataa ataatgacca taaacggttt aatcccctat
     2821 aaaaccgttt aataaaattc acgacgtgaa tataattgtt cattattccc tcattgtatt
     2881 tggcgttatg acaaatgcca taaaatactg tataaatata caggttgtgg cattgggggg
     2941 aagacatgaa tattcaagaa tctataggcg aactaccgga aacatgccgg gctgttatca
     3001 agcgtaagga cggacacatc gttggtgtgc gtgttctgac cgatgatgag cggattgcca
     3061 gcctgatggc gtttctcgag ttggcagaaa tagctggata tactattaca ccccctgacg
     3121 cgtaaaacac ggtataatat cggtgctgga ttgaacaccc ggcaccattt tctgaacact
     3181 gccgcgccac ctggagttaa ccatggcgca gcattcattt atcagggtat ccggcggttc
     3241 gctaataccc gcgacaccag acacgcaacg ctggttgact gaacgagtca aaccaggtgc
     3301 tgttgtgtat gcagatttca aacaggcgcg taatcccgcg tttcatcgta aatttttctc
     3361 acttctcaac ctgggctttg attactggca tccgtccgga ggggctattt ctcctgcgga
     3421 gcgcgaactg gttcacggct acgttaagtt actggcgtat tacggtggac acggtgatgt
     3481 catggcagag ctggctgatc agtatcttct cgatgagtcg gaaaagcgcg cggggagtat
     3541 cagtgcggtg aagtcgttcg aggcgtttcg cgcatgggcg attatggaag ctgggtttta
     3601 tgacgttcat cagatgccag acggcagttt gatgcgcgta cctcgttcaa tctcgtttgc
     3661 agcgatggac gatcttgagt tcggtcaact gtattcagcc gttttagatg tgctgtggaa
     3721 ttatattttg ttccgcacat ttgcctctca ggaggccgct gaaaatgccg ccgcgcagct
     3781 gctggattac acatcatgaa aaaaatcgac ctgagaaaag ctgcacgcag tcgcgcttgt
     3841 actgtgcgta tccccggtgt gtgcaatcac aatcctgaaa ccagcgtact ggctcattat
     3901 cgtctcgccg gaacgtgcgg cacagccatc aaacctcacg atatgcaggg cgctatcgcc
     3961 tgcagtgcgt gtcacgatgc catcgacgga cgtacaaaaa cggattacga gcacgactca
     4021 ttgttgttga tgcacgctga gggagttttc agaacactgg ctatctggcg cgatgaggag
     4081 tttatctgat gagtaacgag tatttattgg aatatacccg cataaaactg cgcgctgcat
     4141 tgcgggattt gtctggtggt tctaaggggc aactggaagc attgtgtgag cacccaccgg
     4201 cagacaaaaa cgcataccca cgcaaacata ttcaccgtgt gcagctggag gaccggaccg
     4261 ttgatgctct ggttacgcca gtttacgctc tggaaagttt cagcagacgt cgccccgcgc
     4321 cgccgatgag tgattttgaa tttgctgatt catcctggcg gcgttctgtg aactcgctgg
     4381 atgcaagtca gcaagcgtgg ttgcgttatt gctacggtgg taacctggca ttcaaacacc
     4441 aaacagctat ttgtgaggct gtctggagtc gctataaagg aaacatcccc gcgtcaactc
     4501 agagaaaagt agttaagcgc ctgctttcgt tggtgtggtt gtccgtgcag gcggtcgcag
     4561 cagcaaataa acgcgaggat tttaaggaga tggccggatc taccctagca ggaatgctgt
     4621 cggtttctcg ttccacctgg tgcgaaacat actccctgca ctgggtagga atgaaagagg
     4681 cggtgagagc acttgatgaa gtggcacttc ttgcaacttt gcatcattat cagaaccatt
     4741 tagacgacgt ttgcgtataa tgcttgcaaa aacgaacaaa ataggccata tttgacgcta
     4801 atttggtatg ttgccaaatt tctaagaacc tcgccacggc ggggttttgt cgtttctgaa
     4861 tcaggaaaaa tcatgtctga acctctaacc gctggcgttg ctgctggctc ggcgggggtg
     4921 acgtttgctg cgttatttcc tgaggcaaca cctgcagtga tgatctgtgc gctggcaggc
     4981 gcagctctct atgtgttgtc gtccgggcag catcgattct ggaagcaggt tattttcgca
     5041 ctcatttcgt ttgttggcgg tgtgtattgc gctgagacag cgtcagccat cattaccggt
     5101 attctgaacg cggtgctgag tcacctgaac cctcccgtaa cagtaaaagt ctctcccgcc
     5161 attggtgcac tggttgcatc agtaatcagc gttacatcac tgttgcggat catgtcacaa
     5221 gctcgtttat ggaaatcaga taaggggatg aaataatgac cctgcactct gtcctcatca
     5281 atgccaatgc aattatttgt ctgatgttgg cactacggtt gatgtttttt caaaaaacag
     5341 gccgctatcg ttttttcatc tcactaactg cttacctcgc gattctgtct gctgcgtgga
     5401 tagccctacg aattttttac gggaaatata cgcaggttga tcccgcagag ttctttctca
     5461 acctcaccat ctgtattgct gtctggcggg cacgagggaa tatttcaaaa ataacaggag
     5521 acaggtaatg acggatccta aatggctaat cgaggctcga aaaaacctcg gcattcggga
     5581 aatgaaaggg aagcaacatg ctgcagaaat tgtgcagtac tggaaagata tcaaacgcgg
     5641 cggcattaag gatgacgaaa caccgtggtg cgctgctttc accggggcaa tgctggaacg
     5701 tgcgggtatt cgctcaacgc gttttgagtc tgcgaattct tatctcgatt ggggtaatga
     5761 actgaaggaa cccgcctatg gatgcattgc tattctgtct cggtctggcg gtggtcacgt
     5821 cggctttgtt gtcgggaaaa atgccgccgg ggatttaatg attttggggg gcaaccaggc
     5881 agatgaagta aatatcaaag cttttcctcg ttcccgcgta acaggctacc gctggccagc
     5941 aggtcaaacg gatgttccac aatcacttcc attggtgaat gctgagaaat ctatctcaga
     6001 agcgtaggta aaacgtgaaa aaacttctct tagctgtcgc gttcttcacg ctggcaggct
     6061 gcacacattc gacatacacc gaagcgactc gcgctgacgg cagcagtatt aaacacgtga
     6121 tgatcgcgcc gggtacgaag attactactg cgaacggtgg ttgtattgat tcaaccgggg
     6181 cagaggcttc atgccttggt ggaaaatagg gtaatttact ttaacgagag gccgcctttt
     6241 tgttttaagg cggcacacat tcaacagatt aaggtaactg atttgaccat tttgttgcgc
     6301 gccagttgtt ctgaaccata atcttctgaa gtatctcatc tgaaggctct gcttcgccgc
     6361 ttagccattc tcttaaagtt gaacaattta gataagagga gttcgcacga taaaaagata
     6421 cgatggcgtc ctctgataac ttctcaggaa acttttttgg gtcagcttct tttaatatta
     6481 cgctcattaa ggcttcaatt ttttctctgt tcattttttg cgttctcatg gttgttgttt
     6541 ttctgtaatg atatcaataa gttcaagata tatacatata tggaaaactt gttcggtagt
     6601 ctatcgatac agcacaaaag gatggctcgc tgccaaagtg aaagcttacg ctgataatca
     6661 gatacacaaa gctgatgtat gcaaacaagt acgccgcgag gctacagttg agtttgatga
     6721 acctgatgcg agcccctaat tcctgtcctg aaagggagat agccccctgg tcgcgggtcc
     6781 tttccgaaat ccaaaacacc gagggtcggt agacgcgcaa aaactcgctc gtttttagta
     6841 ttttttcatt ttgggtattt ccggttccgg tgaggatttt taatggcaag tcaggctgag
     6901 gttgcagcac atttactgct atctgatcgt cgtctgcgcg atctcgcaaa acttccggga
     6961 gcaccagtcc cacaaggacg tggtgattgg gagctagacg catggcgcca tttttatatt
     7021 cgttatctcc ggagcaatag acgcgacaca actagtaatg acgaaccgga agcgggggac
     7081 aattctcccg aaaaaaatcg cgagcagtgg ctgaaaaatg aggaacgaca ggagcgaatc
     7141 ctgatggcac gagtaaaacg ccgcattctc gctaaacgct acgcgccaat tgaattaatt
     7201 agcgtcgctg tatctcgcgt cgccgttgaa ttacgtaccc gcgtcgagtc gtggccacca
     7261 cgcctgaaaa aggtctggcc ggagatgccg caagaggcga gcagtgtatt acgagaggag
     7321 ctggcgatag ccttaaatga gttgtcagac atacgaatcg acttcagcga ttacgatgtc
     7381 agcgatatcg aacgcgatct cgacagggtt gaatcccttg cgcgtgacga taccgatgac
     7441 gggtgttgag tgggctgata aatattttta tctccctgag ggctccagcc acatcgctgg
     7501 gcactggacg actcagccgg tccaggtagt gatgctcaat atgatgacga acgacgcgat
     7561 aaaaatcgtg tctgttcgca aatcagctcg tctcggttat acaaaaatac tcgtcgcggc
     7621 gctgctctat ttcgctgagc acaaaaaacg tagtgccgtg gtctatcagc ctatcgatga
     7681 cgaatcggat ggatttgtcg ccgacgaggt tgaccccgct atcgccgaaa tgccagtgat
     7741 tcagaaaatt ttccccgact gggataaaag caacgagcgt aacaatctcc agcgtaaaga
     7801 aatgagcggc gcgattcttg attttcgcgg cgcgagtgca ccaggaaatt tccggcgact
     7861 aacgaaacag gttgtcgagg gtgacgaagt tgacggctgg ccgctggaag ttgccaaaaa
     7921 aggcaaaggc gagggctcgc ccatcgaact ggcgctcgtt cgaattaaag gggcagcgta
     7981 cccgaaggcg atttttggtt cgacgccgac cgttaccggc aaaagtcata tcgaaatgct
     8041 cgaagacgct gccgatctga cgtttcgttt ttacctgaca tgtccgcatt gtggcgagga
     8101 gcaggtcctg gtatttggtt tcgacggtat cgaatacggc ctcaaatggg ataacagcct
     8161 gcagaccaat gaggcgaaat cgtcgtccgc gtattaccag tgctgccact gcccggaaca
     8221 tttttactat cgcgatctcg aaaaaatgga gttcgggggg cgctggatag ccgaggactg
     8281 cacctggacc cgagacggca ttcatttttt tgatcatgac ggtggcgtcg ttcgcgcgcc
     8341 gaaacacgct gcgatcgtga taaacgccct gtattcacta aacctcgatg gatggggcga
     8401 gattgtcagc gagtggctga aagcgaaagg cgacccgctc aaggaaaaga cgtttcataa
     8461 cacgacgctc ggcgaactct ggagcgacgt ggccagcgag cagctggagc acgatattct
     8521 ggttaatcgc cgggaaaaat acgccagcca ggttcctgac ggtgttgttt atataaccgg
     8581 cggcatcgac tctcagacgt ccgggcgcta cgagtgttat gtgtggggct ggggagccga
     8641 ggaggagtgc tggctgattg ataaaacaat cgtcctcggt cgctacgacg aggaggacac
     8701 gctgcagcgc gtcgacggag tgattcgcaa acaataccgg cgcagcgacg ggaccacaat
     8761 cggcgtcagt cgctgggcgt gggataccgg tggtatagat gcgcaggtcg tttataaccg
     8821 ctcgctgaaa ctcggtccgc tgtgggtcat tccaatcaag ggcgcgagct catacggcca
     8881 gccggtcgta aatatgcctc gtacccgtaa cgcgaataaa gtgtatttgt cgttgattgg
     8941 gacagataca gcgaaagacc tgctcgcaat gcgcctgcag ctggaacccg actccaaatc
     9001 ggcgacacca ggtgcgattc attttcccaa cgacgacgaa atattcagca cgacagaggc
     9061 aaaacagctc gtctccgagg ttctgatccc gaaactcatt aacggtcgcg tcgtttatcg
     9121 ttgggacaac cagggccggc gaaatgaggc gctcgactgc tgggtgtacg gactggcagc
     9181 gctgcgtatc agtaaaattc gtttccagct caatctcgag acgctcgctg agcaacggaa
     9241 aaaatcacaa aacaaactgt ctctcgagga gatggccaga atgctcggag ggagctaatg
     9301 acgtcgcgcg aggttttaac agaacgactg ctggaagcgg aaatcgctct gcacaaatta
     9361 ttaacgggta aatcgaccgt gtcgctgtct cacggcgatt cagcaggaaa taaccggagc
     9421 tatcaatact cgcaggcgag tattgagcag ctccgaacgt acattatcga gctgaaatca
     9481 cagctcggtc tgagtacggg acgccgtcgg cccgtgggag ttcgattatg actgctccgc
     9541 agcagctgct cgggcctgac ggtaaaacgc cactacgccg ttacgcgggg tataacggcg
     9601 gcggtcccgg tttcggtggc cagctgattg actggaacgc accacagcag agcgccgacg
     9661 cagcactgtt gccgaatttt tatcgcggta acgcgcgagc agacgatctc gttcgcaata
     9721 acggcgtcgc gtcgaacgcc gtgcagctgc accaggatca tatcgtcggc aatctgttta
     9781 agttgagtta tcgccctaac tggcgttacc tcggcatatc tcgcgaggac gccagggcgc
     9841 tggcgcgtga cgtcgaggta gcgtggactg aatacgccga ggaccctcac tgcacgattg
     9901 atatcgagcg gaaacggacg ttcacaatga tgatccgcga gggcgtggct actcacgctt
     9961 tcaacggtga aacctgcgta caaccagtct gggagagtag tgccggcagc gtttttcgga
    10021 cgcgattcaa aatggtctca ccgaaacgga ttcgaaatcc gggttacgca gctgacactc
    10081 aatttcgccg cgccggtgtt gatatcgata aaaacggggc cgcggtcggg tactggatag
    10141 ccgaggatac ttaccctctc ggcggagtcg gtaaatgccg gcgtatccca gcacagctca
    10201 gcagcggcag acacgcattc atccacatat tcgagccgct cgaggacgga cagacccgcg
    10261 gggacaatat tttttacagc gtcatggagc gcctgaaaat gctcgacaca ctgcagcaaa
    10321 cacaactgca gagcgccatt gtgaaggcga tgtatgccgc gacaatcgaa tcagagctcg
    10381 acagccagca ggcgtttgaa tatatcgccg gcgcgggtac tgatatcgac tcgaacccgc
    10441 tgaattcgtt tattcagagt tacgtgacgt actacaacgg cgcaaatatc aagctcggtg
    10501 gtgtgaaagt tccccacctg catcccggcg acaaactcag tttacagacc gctcagaatg
    10561 ctgatgctgg ttttagttca ctggaaaaat cactcctgcg ttacgttgca gctggcgttg
    10621 gcgcgtcata cgaagaactt agccgggact acagccaggt cagttactcc agcgcccggg
    10681 ccagtgctaa cgtcagctgg cgatttttca tggggcgtcg acgttttatc gccgcgcgac
    10741 aggcgtcgct gatgttctgc tgctggtttg aagaggcgct ggcacgcgga attatcaccc
    10801 tgccacgctc agctgtccgg tcgttttatg aggcgcggaa ctcctggacg aacgcgctct
    10861 ggatcggcgc tggtcgcatg gcgattgatg ggctgaaaga ggttcaggag agcgccatgc
    10921 gcatcacaac tggcctcagc acgtatcaga acgaactggc gctgcaggga caggattacg
    10981 aggaggttat ggagcagcag gaatacgaaa ttcagcgccg gcgtgaaatg gggctgagcg
    11041 aaccgtcatg gtctgtatct cacccctcaa ataccaacga taacggctgg ggaggtaact
    11101 gatgccgtgg aacaattttc cgcacctcgc cgccagggcg ttcaatcaac cgcttttgct
    11161 ggagcccacc tacgcgcggg tatttttttc ggcgctgagc gaccggttcg gtaccgggcg
    11221 actgattgat acagcgtctg gagaggtaat gaacagcgac gaaatgaacg cgctcgcgat
    11281 ggggtgggac agcagcgagc gaacacgcca gaaatcgtat cgcgtggagc gtggtatagc
    11341 cgttctgccg gttaccggga cgctggttca taaattgggt tatatcaacc cagttagcgg
    11401 gatgagcggt tacaacggaa tcgcaaaacg cctgcagcag gcgatttctg atcccgatgt
    11461 aagaggggtc ctgcttgata ttgactcccc tggcggtgaa gtcgccggcg cgtttgatac
    11521 tgctgattta atcgcccggg cgcgagagca aaaacctgtg tgggcgctgg ccagcgatac
    11581 ggcctgcagc gctgcctatt tgctggcgtc agcgtgttcg cgccggctga taacgcagac
    11641 aggcacagtt ggttcaatcg gtgtcctgat ggctcaccgc tgcgtcgaaa aagcgctgga
    11701 gattgctggc gtcgacgtga cgctgattta cgccggcgcg cacaaagttg acgggaaccc
    11761 gtattcccag ctgccaggcg acgttcgcga cgaattccag ctgagtatta acagcacgcg
    11821 cgagcagttc gcgcaaaaag tctcggatta taccgggctg aaaaaatcca gggtgctggc
    11881 cacggaagct gcagtattta tcggcgcgga cgcgattaaa tctggtctcg ctgatcaact
    11941 cgttaattac gcggacgcta tcgcagtgat ggccgacgca ctgaaaccaa aaatggagcg
    12001 ctttatgtct ggtacagaaa ccacggcgga gaccacgacc acagaacaaa ccgcgaccac
    12061 gactactgtc gcgcaggttg agtttaacac ggagcagatt cgcgcggacg ccgcatcaag
    12121 cgaactggcg cgtgtgatgg ccatcatcaa ttgtcccgaa gctgttgggc gcgaggcgca
    12181 ggcaaaagcg ctcgctggcg tccccgggat gacggtcggg caggcgcagg tagtcctcgc
    12241 tgcagcacct aaaacggcgc aggcgcggac agaaacggcg ctcgatacac taatgagcac
    12301 tgaatcacca gcgactattc aggatgctgg cagtactacg gcaacaggaa caaccgcaaa
    12361 cgtctcgatg ctggttgctg cagggcgttc aattttaggg gatgaataat gactacagaa
    12421 acgtatagcc cggacgattt tattcttggg cctgaccttg ttgtcacgac cgtcggtcat
    12481 tttcagggcg ggatcaacgt accccggctg acaccgatta tgctcgacgc tactgtcggg
    12541 acgtttaagg tttgggacgg ctcagtcggt aaagctattg gcctcactgc caccgcagtt
    12601 aatactggct ccagttctgc agactgctcg tactataaat caggctcgtt ccgttacacg
    12661 gcgattaact ggggtacggt taccgacgta gctaaacgca aatcagcatt tgcgggtacg
    12721 ccgatcagcg tcggctgata gcaaaaaact caaaacaagc cgcctccggg cggttttttt
    12781 ataacaggat atataaatga gcgattcgtt tactacgtca gaactgatta ccgcgacaca
    12841 gcaggtattt aagttcaatc cgttgttttt aaagctgttt ttccgtgaga cctacacgtt
    12901 tacgagcgaa gaggtttttc tggataaaat cccgggaaaa gtgaacatgg ccgtgtactg
    12961 cgcgccgatg atcaccggca aagttgaccg cactcgcggc tattcaacga accatttcaa
    13021 accgggttac acgaaaccca agcacacgat caatccgaat atgagcatca agcgcgcagc
    13081 cggtgagcag attggccagc cggaaacgcc ggtcgaacgt cgtgcaaaaa tcatcatgca
    13141 gaacctgctt gatgaggaac tgagcatcag ccagctcgaa gagttccagg cggtacaggc
    13201 tgttctgtac ggtaaataca ctgtttccgg cagcaatatc gagacctatg agatcgacat
    13261 gagccgcagc gcgacgaata acgtcactca gtccggttcg acagcctggt cgactcagga
    13321 cgcggaaacg tatgacccga gcgacgatat tgaatcctac gcggacctcg cctccggtgc
    13381 ggttaacgtg atcatcatgg atggtaaagc ctggaagcag ttgaagcgtt ttaaaaaatt
    13441 ctggacggca ctggatacgc gccgtggctc aaacagccag ctcgaggtcg cgctgaaaaa
    13501 cctgggcgac gttgtcagct ttaaaggcta ctacggcgac actgcgctgt ttgtctacaa
    13561 agggcaatac ctcgacccgg taacagacac tgaaacgcgt tatatgccgg ataacacgat
    13621 gatccttggc aacacaaaaa accgcgggct ccgcacttat ggcgcaattc aggacgaaga
    13681 cgcgctgaaa gagggtattt ccgaagcgac gcgctatcca aaagtctgga ccactaccgg
    13741 tgatccggca gtgacgcaaa caatgacgca atccgcgcca gcaatggtcc tcacggacgc
    13801 cgacgcgttc gttgtcgtaa aaatcgcgta agagtcgaaa ggctcttttt tgttaaggaa
    13861 tcaacatgag cacaaaaaca gaattgctgg cgcgtattga cgatctgagc gctcagctcg
    13921 gtcgcgaatt accgcgcagc gggactattg cggaactgga atcaatcgtt gccggcgctg
    13981 agtcagagct cgatattctg aacgagcaat ccggtgacgc gggaggcatc gagattacag
    14041 tgaatactgc gtccgggacc gataatgaac tcgctcaatc aatcgcgagc gctatcgttt
    14101 cacagtcaca agagcaggag ctcgcaccgg ctacgcgccg cgtcaagttg cgtaacacgc
    14161 tggacgttta tcactacgtg aacgggcgtc gcgttcgcga gattgttgcc gcaggccgtg
    14221 aaattgttgt tgattcaccg gaggtcgcag accttatcgc agctgatcac gtttacgctc
    14281 tatgagctac tacgatgacc tccgggccgg cgacgaggag atgatccgtg agtggggacg
    14341 gcccgttaaa ttacgcggta aaaccgaccc cattatcgct attttcaacg agccttacgc
    14401 gcgcgttgac gttcctcacg ctggttttat taccggtaca gtaacgagcc tgacggcgct
    14461 ctcggacgac gtcgctggcg ttgttgcgcg tgacgtcgtc caggttccaa agcagcgcag
    14521 catcgaacct gacggctctg ttacctggtc tggctggacc gattacgtgg ttaaagagcc
    14581 tcaacctgat ggcacagggc tcacgaatat ttttttagag cctcacacgt ccagcgaaaa
    14641 cagcgagtat tcaaaatact aagtgggacc tcggtcccac ttaaggggga accgtggccg
    14701 atttacgtag taacgcgcag atgttcgata tcgatgtttc ggcgctggag cagctcagag
    14761 tcgaaatcag cgcgacgcaa catcaaatgt taatggtata caacagggcg ctgaaccgaa
    14821 ctgcaaaaca tatgcaccga atttcagcag gaatgatttt gactgcactg gcagctaaaa
    14881 atcataaggc cgtagataaa cgaatcaagc cgttcattaa gcgccgtaat tttacaaaag
    14941 aaggggcagg agacctgagc agcgccaagc tctggtacgg tctgaatgat ttccgggtat
    15001 ctgagctaaa ggggcgatta caaaatccac ggaggcaaaa acagcctcgc gatcttgaga
    15061 ccggcctgtt tttgaaaacc aaaaaaggcg ctagaggggc aacttttaca cccaaaagtg
    15121 cagggctggc gatgatgagt tggcccgatt cattcgtagc gaaacgctac ggtgcgaaaa
    15181 gtgtctggat tcgactggat cgcggaggaa tcgaagaggc tcgcgtacct gtgcacgacg
    15241 cgctggagga tgctatcgat gattatattt ttgaaaacat tggccctgtt tttatgggct
    15301 tttttgagaa agatttgcgc ggtcgcgtga aaggtaatgt ccacgtagac ccaaaaacag
    15361 gtaaacgatt atgagcggac tggatgcatt tgacgaatat cttgatcgcg ttaaaggcgc
    15421 ggttttacaa ataccgttca tcaaaacatt cggaatttat ccggaaattc cggctggatt
    15481 tgaaacgcca gctctgttcc tggaaatcag caactggtca cagagtgacg aatcggttcc
    15541 aggctcaatt cagtcagtcg agctgtcgtg taatttgtat ttgctgcgtg aatttgcagc
    15601 tgatcagtac ggactgaaat cgcagaatgc agcgctctat atgacgagct ggatcaatgg
    15661 gcgaatgttc ggtcccggaa ctaaaccggc aaaattcagc gacgcggaac cgtgcgactg
    15721 gattaaaaac gggcagtcag tcggttcgca ttcagttcag tgcgtgtcgt tcacgcaggt
    15781 tgtaggcgtt ggtccagata ttttcgatta tccctcacag gggaccctga aaaatatata
    15841 cgtcggaata gcaccagata tcggcgcaga acatgagggc gactattatg gcccaatcgg
    15901 acgatgaata tgctgcagcg gagaacgcgc gcaggctgcg tgacgcagtt aaacgcggca
    15961 cgatagctgc agttaaaatg aatcctccac gctgccgagt ctcatttggt ggcgaacacc
    16021 agtcaggctg gctgcagtgg tttactcacg ccacctcaga acgcgtcgac tggagcgcac
    16081 catcagtggg cgatcccgtt actgttgttt ctgagggtgg ggatacgcgg aacggtgtag
    16141 ttatgctcgg gctgcacatt gacgacaaag ctccgccaag caatgacccc catgatcatg
    16201 tcactgcata ctgtgacggg gctacgatga cgtataacac aaaaaatcac actctgacat
    16261 ggcagggcgt accggacggc gtggtaaaaa tactcggtga gtctgaaata gaaatatttg
    16321 gacgcgcaga tgttactatt aatagcgaaa atgtagttaa tatccatggt gggaaattaa
    16381 ttaacgcaga cgctgatatt attaatgtca cagcgacaga tacaattaac gcacatgctg
    16441 atttagtgaa cgttatagca acgagttctg ttagtgttac tgctgcgaac agaatatcgc
    16501 tgacagctca aacaatcagc gcgtgggctc cgggtggtat aacactagct ggtccaacgc
    16561 atattaccga gacattagta gtagataaat tagcgacatt ccgtaacgat atttctgtca
    16621 ctggagataa cggtggaaca ggtaatatca caactcgcgg tagtgtgtta gcagggcaag
    16681 aggtgcagga ccgacaaggc acaataactg aagtccgcac aacgtataac ggacacactc
    16741 atatatgtcc ggacggggaa actcaacaac cgaaccaacc aatggcgtaa agatgcttgg
    16801 aatggaccgt aacaccggta aacttttatc ggggactgat cacattcgtc agtctatcgt
    16861 tgatatttta acgaccccgc tggggacgcg tgtaatgctg ccggaatatg gcagcaaatt
    16921 atttgatctt gttgataatc ccacagatcc gtcgcttgct atgcgaataa tcatggaaag
    16981 cgctggcgca atagcacgat gggaaccacg cgtcagaatt gacagaataa atgtgttagc
    17041 agtggatatc gggaaaataa caatattaat tatcgcaaca gatatcgaaa cacaacagcg
    17101 attagagttt aataatatgg agctgatatt ttgataacat caacagttca gaattcgata
    17161 gtgaaaacta ttgatatgag tctgctgccg ccgccagcat tcgttaaaac cccgttattt
    17221 tcagatgtta aatctaatct actgtcagag ctgcagatat tatatccaca atttaacgca
    17281 ctcctggagt cagacccggc cgttaaactg ctggaaatag ttgcatacag agaaatcatt
    17341 attacagctc gggtaaatca ggggatgctc gctgtattgc tcgcgtttgc aaaggggagt
    17401 gacctcgacc agattggcgc taattttgac tgcctgcggc tgttgataac gccagctaat
    17461 cccgatgtaa ttcccccgac tgaggctgtt tacgagagtg acgacgagta tcgacaccga
    17521 atacagctat catggtatgc gcggaatact gccggcagta cgaacgccta taactatttc
    17581 gcgctgtcga gtgatcccga cgttctgtca gctcaggcgt acggcccgcc ggtgacccag
    17641 cctggatacg tcgatatgta tgtcttgtcg cgaaccggcg acggcactcc gcctcaatca
    17701 ctactgaata cagtgaatgc ggccctgtct cctgacgata ctcgaccgct gactgatttc
    17761 gtgactgtaa aacctgcgtc gaacctgaat taccgggttg aggccgttat cgtagccggg
    17821 ctcggacccg accagaacgt tttgcttaac ggtgctcaga gcgatttggc gatatatgtg
    17881 gatacgcaac ataaaatcgg agcgacggca gctctgtcag gaatttacga cgcgatacat
    17941 cgtgacggca ctgaacgtgt gattctgata tcgccaacag aggatgtgat cgctggcgtc
    18001 gggcaggctc cgtactgcac agaaatcaaa ctcagcgtgc aaatggggta gccatgacaa
    18061 gtcaaagcgt actgcctccg aacgcaataa cccctgagag ggcgcttgag gttgttttat
    18121 ctcacgtcgg cgatctgcct ggcgatattc gaattattaa aaatcccgat ttgtgccctg
    18181 caaatctgtt gccgtggctg gcgtgggagt acgcggtcac ctactggaac cccgactgga
    18241 gtgagcagca aaaacgcgaa attattaaag cagctgcgtg gcagaataag caccgcggaa
    18301 cgcgtggagc tgttgaacga gcgttattaa ctgttggctt tgagagcaaa atgagagagt
    18361 ggtttgaagc cacgcccagg gacgacccgt atacattcgc aattaaaata tatctgctaa
    18421 aaagcatggg gttagattta gaactattga atacatttat tgcacaaata tttgatgcaa
    18481 aaaattgtcg ttccttatta aaagaaataa atttcgaaac gggtgtcgat ggtgagtttt
    18541 ttatagccgg tacaccttac gcgaaaatag gcgtaaacgt cccagcagac ggcgacggcg
    18601 gtgtaaaaat taacggcggg ctatatatat cgggctcacc aattgttagt ttaaatgtgg
    18661 agatagggcc aaatggctga aaataaaatt tacgcagtac tgacagaccg tggcgcacaa
    18721 cttgaagccg ctgcgctggc atcgggtgta cccgtggtgc tgaataaatt cgttattggc
    18781 gacgcaaacg gaaacgacga cgtaacaccg gacccggcac gaactgcatt aattcacgag
    18841 acgtatcgtg gggatattaa atccgcagaa aatagcggta accaggttat ctttacactt
    18901 tacgtaccgc cagacactgg gggctatact atccgtgagg tggggatatt aacagataaa
    18961 ggcgagctgt actctgtggc gcgctcgccg gacattttaa aacctacgga cagcaacggt
    19021 gcgctgattt caatcacgta taaatacaca ctcgcggtgt ccagcacatc tacggttaat
    19081 gttgtagtat atgacaacta cgtaactccc gaggaagctg acaaaaaata tttgcagata
    19141 agtaaaaact tatctgaaat tcccgcaaat ggtgaaactg ctcagcaggc tgcgcggaaa
    19201 aatattggta tagatgggga cgttgcatat agagataagg gtaatgcatt tactcatcca
    19261 aatactttta aagatgatgt gatttttgaa gacgttttat cctctaaaaa acaaataaat
    19321 ataaacaaac cgggcactga tttgacgttg ttttcatttg ctggtttatc agatgtgtcg
    19381 ggcgattatt tagatttttc gacccgtgga gaaaacaatc agaaggtttt ttatagatta
    19441 cgcggagttc actatgatgg cctggtgtta ctgtgcggcg ggcagacgta taaatactgg
    19501 aatgaggcta atcttaaacc ggttaaaacg attaacgggg agtcgccgga tgaaaatggc
    19561 aacatagata taagtctttc accggaccca tcaccggaaa taggtgttgt tggcgcggtt
    19621 ggtacttatg cgcttgtcgc tattaatcat gcaccacaaa tactcccggg ctccatatgg
    19681 gctggagggg atttatatta cgctagtata aataataccg gttcatataa cacggatatt
    19741 gccacaaaac cattttatga cacaccactt attggctcat ggcgagccat ggcgtattca
    19801 cccggtggag tcggtgatat tgggactgcc tgttttttag ctattaggat tgcgtaatga
    19861 taagcgttga ttcatttaga aaaataacaa accctgtttt taatgacgag catgggttat
    19921 ctataaatat tatttgcgat attgacggga ttggaaacga cattccattc accgcaatga
    19981 aaaatgattg cgaggaacac ggtagggttt tatatgaaag agcgctgagc ggggattatg
    20041 ggagcatttc taaatttgta aaaaaagagc agacgcaaaa actccagact acattacgga
    20101 ctgttggcga tctgtcaatt gaggcattct caattcagtg tgcagtagat gccggaaccg
    20161 catctgacgc gcaaatcgaa aatttggccg agcttaaaga agcaatagcg acgcgaattg
    20221 gtgaaaatag agaaggaata aaatgagcgt aactaatttt ttacacggta ttcgcacgct
    20281 cgaatacgac gacggcacaa aagagatcaa cacagtagat atttccgtga ttgggattgt
    20341 tggtactgct cccgattcca gctacccaac ttgtgcatca ctattatggg gatcagagct
    20401 cgcagacaat cttgttgagt tttcgactgt catgccaggg gccgacggaa acaactggat
    20461 tgttgaaatt gttgatttgg gaattaacgg gaatgtagca actccaggat acagcacact
    20521 gcccgacggt tcgagaaaat taacaatgat cacggacggt gccatgaccc cgtcaaaaat
    20581 gtacgatcag aaccagcagt ataaagaagg actccagatt ggagaatata ttaacgtcac
    20641 attcggtggt gatcacgcag gaacggggac tgtattcgcg ctccccccaa caaatttatc
    20701 cggtggtaaa gacgaatcgt tcaaatataa tgttccgacg ttaattgcag gcagtcaaaa
    20761 aaaagctgga ttgctcggtg ggtctggaac ccttcctccc gctgtatctg aaatactaaa
    20821 tcaggaggat gccattattg ttgttgtccg cgtcgaagag gacagcgacg aaacaaaaat
    20881 gcggcagaac gtcgttaacg ggattaacgc attgttgacg tctgcgcaaa taaatcaagt
    20941 aacgccgaga attttaatcg ctccagatta tagtgctaac gattatattg cagaacaact
    21001 tgaagtggtg acaaataaat tacgaggcgt tggatatatc gattcaccgc gaggcgctac
    21061 gcctgctgac gttgtaaatc gtcgtcagag gtacggcggc agaatggaaa ttctgcgccc
    21121 tcgtgtttat tctacgagtg atgttagtgg tttatctcgt ccgtattcag ctatcgctgc
    21181 tggtttacga gccagaattg ataacgaaaa agggttttgg tggagcaaat cgaatcaaaa
    21241 tatctacgga attacaggac tcgaacaagt cgatgatttt ataatcggtg aaacgaactg
    21301 cacagcaaat ctgctgaacg ccagccaggt cagcacaatt attcgctatg atggattcag
    21361 acactggggt aactatctgt gtagcctcag tccccaatgg tcctttgaat gcgttcgtcg
    21421 gacagctgac gtaattgagg attcaatagc ccgggcaatg atgaccgatt ttattgatcg
    21481 cccgatagac ctgcacctcg gaacagacgt tgtagagtcg ataaacgcgt atctgcataa
    21541 attagaggag cagggcgcga ttaacggagg acgggcgtgg ttagatgaag agttaaatac
    21601 aaaagaaagt ctggccgctg gtaatcttta tattaatgtt gactttggac cgaaatcccc
    21661 ggcacaaaca attacgttaa tgtaccgcat taataatgat tacacagttg aagctctggc
    21721 ctctcttttt aaagaaacag tctaataatc tggagaattg atatggcaga tagtaacacc
    21781 tatcgtgcat ttgcgttgtt cgtccaggga gaacgagttt taaattgcac tgaatacact
    21841 cccgtcgata tgaaaataat cgaagatgat tttaagacgg gcgctatgga tacagctatc
    21901 actctggacg ggggaatgga gaaaatgtca gccagtttta aagtctctgg ctccgattct
    21961 ggtgtgatgg ggtatttggg attaatacca ggtgctaaaa caaggttcga aattcgaagt
    22021 gcatatactg acaattacgg tgtcaatttt gaacgtattg atacatatga ggggcttatt
    22081 acggcgatta ctgatgacgc gcaggggact gattcaaaat cagcagttgg gcaatcagta
    22141 acgattgcgc cgagctatta taaacgagtt caaaatggaa aaattattta cgagattcat
    22201 cctgcaaaaa tgaaacgagt aattaatggt gttgacgttc ttgcgggcgt tgctcgtatt
    22261 ctccatgtat attaaaaggc aaataaaatg gaatcattat tagacagtat gactattacg
    22321 ctctctcgcc cgtttattat taagggggag agccgcgata caattactat tcgcgaacca
    22381 aaattacgcg accgcattat gttcagtaac gataaaagtg gtcttgaaga gcggacagcg
    22441 acaatgctgg cgcgcctggc aaatctggag cgtgaggatt tatatgcact gcctgcgtgc
    22501 gattacgatc aaatggaggc cgcatttaac gaactggtaa agcctccgaa agaccgacat
    22561 cagatatagt tattctgatt ccgtttattg cgaaaaaact tgccatcccg cccgatacgc
    22621 aaatggacct gccgtaccgg gtttttaatt tctatgtaaa cgaggtcatg aaaatcgatg
    22681 gctatttctc aaaactttaa aacgcaggtt gtattcggtg ggcgaataga cccgtcgttt
    22741 cgtcgcggta caacagaact caatgatgcg attcggcaga cgtcgtctac tgtcgggaaa
    22801 ttaacgaaaa gtcaggataa attaaaagac aaaatcgcag caatgaaact ggcaggtaaa
    22861 gacgtttctg atttatctgc tcagtatcaa aagctggacc gtcgaataaa agcgacaacg
    22921 caggaccagg aggcgctgaa tactcaactt gctaaaaaac agcggctgga gaagtggacg
    22981 ggtcgcgcaa aaggagctgc aaaatggggc ggacgagccg ccgcaggtgc ggtgagggct
    23041 acggggcggg gtatcaaatg ggggacactt ggcgcggcag ggctcattgg cggtgctgca
    23101 gctggtgcgc tggcaatgaa tgcagagacg tcagaaaaac tcggtctggc gaagtcctac
    23161 ggcgttggcg tcgaaaaata cgctgcgtgg gaaaatattg gtaaagccgc gggcctgaac
    23221 ggcgaaaata tcggggattt gtctgaagaa cttacaaata aaatcggcga aatcggaaac
    23281 gaaaaaagcc tgaatcccat gttatttcaa attggcctga cgaaaaaacg aatggctggc
    23341 tgggatcggg aaaaacagtt taatgaggtt atgcgtcgca tctctgaaat gaaagatgag
    23401 aagcaagcgg cgagcctcgc tgaccagctc atggggggcg aggctaataa gatcatgacg
    23461 tatatgaagg cgacgggtaa aagctgggaa cagacaatgt ctgacgcgca aaagtcgaat
    23521 ttgttaacaa aagagggtgc ggaaggggcg gcgcgtgcgc atgtatcagt gacaaatctg
    23581 tggggctcta ttacgtcggg acttgcagat acactcggta aaatcggggg ggaactcgcg
    23641 ccgacgtttg actcagtacg tgaaacattt acatcctggt ttaaggataa tcagggtggt
    23701 tttgtcagca caattactga gtgggtaaaa ccagaaaata tgaaaaaaat gtgggatggc
    23761 atcgtcaatt ttggagaagg ttgcgtcaaa ttcgggaaga ttatatgggc tgtcgtaaag
    23821 aaactggagt ggcttattcc ggatgaaaaa tcagatgaag aacagaaaac ttataatgac
    23881 gaatataatc gggcatatca agagtttatg gattcgggag gtaaatattc accgaacgct
    23941 ggattgactg ctgataatat tgcgaaggca aaagccgaag aggctgtcga aaatatgcgg
    24001 catccagaac ggcaagaaca ggcaaaatct caggcagaat caatgctcgc gtttgttaat
    24061 ccattatccg ggatggtaaa taaaaatacc gcgcctgcag cactggattc gtcgacgtta
    24121 aatattgata cgttgaagca ggcagtctcg acacctgcgc cggagcaaaa caataaaatc
    24181 gaaatcaata ttgtcggtgc gacagatccg cagtcaatac aacaatctgc agcgtcgggg
    24241 gtgctcgatg gactgagaca ggctgcgagt tcgtacaatc gtggcgcgat gtttgataaa
    24301 ccagctgcgg cggggtgaca atgagtgatg caataaacgg tgctgaggat atcatgctcg
    24361 gcctgggtga tttcattttt gctatatcaa cagtagcgta caacaaactg caacgtagtg
    24421 acgcctggcg ctgggctcag caaacacgat tcgggaaaaa cgacgcgttg cagataaccg
    24481 gacgaccgaa ccccacaatt actatcgatg gaaagataaa cgctttattt cttgacgggt
    24541 gcggcgtcgg cctgttgaca gatttgcgag cgctcgggaa cacgggagaa ccccagcagc
    24601 tagtgctggg gaccggggag gtgaaagggt actgggtgct gcgagaacta actgaaacgc
    24661 aaaacagctt tttgatgggc ggaacaccta aatcgcagga cttttcactc acgctcgaat
    24721 attatggagc ttcgctagat taatacaaaa tcaagcgcta ccagagcact taacaagtat
    24781 aaaaataata aaaaaaacta cgaacataaa tgtaaaacaa cctgatggta ttttttttga
    24841 cgccgaaata ctaatacctc tccgactact aaaattaatt ctcattatga aatcctttta
    24901 attaatgggg ccccaaatga ttacatacac tacacgggac ggtgaccgtc tcgatcagat
    24961 ctgccttgcg gtctacggga gagcgtcaaa aacgacagag actgttttat atcaggtttc
    25021 gaattacggc gtaacggata tgtgtgccgt gtttcgtgct ggagaaaaaa ttgttttacc
    25081 agatatcgaa ccagagccgg ttaaaaaaga aacgcaatta tgggattgat gaatgagtga
    25141 ttacgtaaat actggcgtgg aggcgtggaa acctaatttt tatatatctg ctgataataa
    25201 aaacatcacg gataaaataa gaaaaggttt gatcaatatc acgctgacag attacggcgg
    25261 ctccagtaaa cagacagacg aactccgcgt agcaatagtt tctgaaactc tgaaaattcc
    25321 ggcccgcgga gtaaaaatca gtattgggct cggtttcggt aatcagataa ttgataaagg
    25381 gatatacatc gttgacggtg ctagcagtgg cggtgaaccc cgggtagttg atttcacagc
    25441 aaaagcggct ccgatgaacg cagcaaaagg atctccgaca gttcagagca aaaagactcg
    25501 ttcctggagc gacgtcactg tcagcgatat cgttgcgaca atagcgagcg ataatgggct
    25561 aaaaccacgc gtatcaaagc ggtttgcaga caatgttatc acgcaactcg atcaggtcgg
    25621 cgagtcagat atgcacctga tgtcgcggct cgcaacgaga ttcgatgcgg tgagtaaacc
    25681 cgcgggcggg tactggatat tcctgccgcg cggcgcgggc gaatctgtta gcggtgctcc
    25741 gctccagcac tacacgctaa ctcgtcgtga taactccagc tggggctatt cgagaaatgg
    25801 acaaagtggc gatagtggta gcggtggcga aaatccggaa ccgacgtatc tgattaaata
    25861 tcacgacaca gcaactgggc agatcaaaga attgcggaca aggtagtggc ggcgatcctg
    25921 tcattgaatg gccggcagtt gagccgtcgc tcgacgcagc gaaagaagct gcaccagggc
    25981 tcaagggcgg tgcagctaaa aaagagttct ctatgacgta cacgacgcca gcgacgctcg
    26041 atttagtgtc gctgacagca gaatgcaaag tcacaactca aggtttcggg accgaggagg
    26101 accgggactg gactatcaac acactgagtc tgacgctcgc tgaaaacggt ttttcggttc
    26161 ggctgtctct ggagtaaatg ggctcggcga gccggtggcg gtatgaattt agtattaaca
    26221 gatgtcgact gcaaacggca cctgcgatat ttttaggcgt aagccggaga acgaagagtc
    26281 tctggtgctg aacgttgtta tcgttagatg ctgacatatt tttaatattg ggagttttca
    26341 aacatggtcg ttaaaaatgg tcttgcctgt gggattatgc ttgctcttgt cggttgtgct
    26401 acaaaacctg tctcaaatga acaagccact cctgttcctg aaaaacaaat tatcagtacc
    26461 gcgttactcg aaaaacgaga cggtacggga gaggtgatca tcaagcgtga ctcgggtttc
    26521 tttggaagcg cgtgtttttc ccgtgtttat gttgatggta aagaggtggc agatttaggt
    26581 acagctcaga aagtcgttgt atacccaacg attggtgatc atgtcttcag cgcgtggcca
    26641 aaaggagtat gtggtggtgg catgagcgaa caagcgggca aggtgacaac agatcgtact
    26701 ttgatgtatc gcatagggta tggatcaaac ggtgaatatg gaattcaccc aaccgcgttt
    26761 taatttgtgt tggggtatgt gagtttttct catacccctt tacctcgctt tgctcctgac
    26821 agtttggtca cataaaggcg cagaacatca ccatttttgc caaaaacctt cagaagacga
    26881 tgagtaagac gcgtcgtaac tatatgatta tttgatattc atacacacca ttatgtccgg
    26941 ataatttgcc ataattattt gaatttattg cacaaagcga ccaatttaaa atccctcggc
    27001 gttcgcgctg tgcgggttca agtcccgctc cgggtaccat gggaaagata agaataaaat
    27061 caaagcaata agcagtgtcg tgaaaccacc ttcgggtggt ttttttgtgc ctgcaacttg
    27121 tcgttacacc ctccttaatt tttaatcacc ggcaaagcca ctcaaaaagt atctctgata
    27181 cggaccggca tgtaagatag gtgctggcga gttgagatcc acaaggaaaa gcgtatgaaa
    27241 acgggaccgt taaacgaaag tgagttggaa tggctggacg atattctgac caaatacaac
    27301 actgaccacg ccattcttga tgtggcggag ctggacggtt tattgacggc ggtgttgagt
    27361 tctccgcaag agattgaacc ggcacagtgg ctggttgccg tgtggggtgg ggctgactat
    27421 gtgccgcgct gggcgtcaga gaaagagatg acgcgcttta tgaacctggc ttttcaacat
    27481 atggccgata ccgcagagcg tctgaacgaa tttccggagc agtttgagcc attattcggc
    27541 ttgcgagaag tcgatggcag cgagctgacg attgttgagg agtggtgctt tggctatatg
    27601 cggggcgtgg cactttctga ctggtcaacg ttgcccgatt cgttaaaacc agcgctggag
    27661 gcgattgcgc tgcacggtac tgaggaaaac ttcgagcggg tagagaagat gtcgccagaa
    27721 gcgtttgaag agagcgtaga tgccattcga ctggcggcgc ttgatctaca tgcgtactgg
    27781 atggcgcatc cgcaggaaaa agctgttcag caaccgatca aagcagaaga gaaaccgggg
    27841 cgtaatgatc cttgcccgtg cggtagtggt aagaaattta agcagtgctg cctgcattaa
    27901 gaggtcaaat tgggtgtcag cgttaattct ggcacccaat aagaatctaa tcaccccact
    27961 tctggtaaca agcctgccgc aatcaaaaat tgcacgccaa tcacagcaat accacagaga
    28021 aacaccacca ccagcgccgg acgaccacct ttcacccggt aacccgcctg aggattgtgc
    28081 ttcctgcttt gccaggtcaa cagcgaaggg ataatcaatg ccagtaccgc cagtgccaca
    28141 ccggcgtaac ccagcgccat cacaaatcct cgtggataaa acagtgcaaa cgccagtggc
    28201 ggcagaaagg taattgcacc agtttgcaat cgtccaccaa cggtatttga acgctgaaac
    28261 aaatctgcca gataatcaaa taagcctaac gctacgccga ggaaggacgt ggcgagggct
    28321 aaatcagcaa ataaatgcac tgccagctca acatgcggag aggccaccat ttcgcgtaac
    28381 gcctgtaaca gcccgtttaa tccggcatga ttagccagca atcccataaa ggttgttgaa
    28441 tcaatgctgc caagggtcgc cagctgccag aaaatatatg ccaccagggg gatcgcacta
    28501 ccgattataa acacccagcg tagcttacga atgttgccat ccatatagct gacaatactc
    28561 ggcacgctac cgtgaaaacc aaacgacgta aaaatcaccg ggattgcaga cagtgccagt
    28621 ccctgttgca acggcagggt taaaagattc actttgtgaa tatgcggtag cagcaatacc
    28681 agcatcacca ccagaaaaat aattttggcg ctgaacagaa aacggttaaa taaatcgacc
    28741 agcgaagttc cgacacaaac cacgccaccg gcaacaaaag tgaacaacag cacgccagcg
    28801 gttgccgaca ttgaaatacc tgtccagtcg ctgatgctgg aggccaacaa ttcaccggca
    28861 ccgctgatgt atgccgcagt cagagcatac attaagaaca tcatactgaa gcccgtcagc
    28921 cactgaccgt agcgtcccag atagcgtttt gccagcgtgc ccagaccggt atctgccggg
    28981 acatgctgat acacctccag cagtaatagc gccgtgtagc acatcaacgc ccaaagccca
    29041 atcaacaaga ttaacgtgac gctaaaacca acacctgccg cagccagcgg cattgccagc
    29101 atgcctgcgc caattgtggt tcccgccacg ataaaaacac ttcccagagt tctgtttttc
    29161 acgctttctt ctgtcctgac gatctttatg agtgatatct gcggcgcagg ttaaggcata
    29221 acggctgatt cgtcaaatcg acgttacata tagtgtaaat ataaatgtac aataaaaagc
    29281 gttcagttct gcgctggcgc aaagtcttca taagcagcaa tcgctacgct aggctttttt
    29341 tgaggaggaa cgatggactc tattcacggt catgaagtgt taaatatgat gattgaatca
    29401 ggcgagcaat atacgcatgc cagtctggaa gcagcaatta aagcgcgttt tggtgaacag
    29461 gcacgttttc acacctgctc ggcagaaggg atgactgcgg gagagttggt ggcgtttctg
    29521 gcagcaaaag gcaaatttat accttcagaa gaaggttttt caaccgatca gagtaagatt
    29581 tgccgtcact gatttgaaac ggcggcagaa aacctgccgc cggagagcat tagttttgtg
    29641 tgtcgagggt agagagttct ttgtcgataa aatacagacc ttcgccactt ttgcctgcca
    29701 ggcttaattt atcaataatc gatttgaaca gtttctcttc ttcatgctgc tcagaaacat
    29761 accattgcag gaaattaaat gttgggtagt cctgattggt cattgcagca tgagccagtt
    29821 cgttaatttt ctgcgtgatt aattgttcgt gtttataggt ttcctggaat aattcatcaa
    29881 gtgaggaata ttcagcaaac ggagattcaa cggtattaat acgcggtaag ttgccggtat
    29941 cagtcaggta atcaaacaga cgctgcatat gcgtcatctc ttcctgggcg tgacggcgca
    30001 ggaacgcggc agcaccttcg aaggtatgat agctgcacca ggcgctcatt tgctgataaa
    30061 gcagtgaaga gtacagttcc aggttcatct gctcattaag tttttcaatc atttctggtt
    30121 tcagcatgat agtgctccac aaaggttata tttgttgctg tagcgtcact ataatttgtt
    30181 attttattat ttgcaaaagg taaaatataa acatcattta ttaaaaatac gaatgggaat
    30241 aaaacgcatt accgctattt atttaatcag cgaagatccc cctcgctgat taaaacgttt
    30301 agagaaagac agggaaggcg gaatgatggc actgatatga caaagtgaat tccgtattca
    30361 aacataagga acccgcaaag aggctacagt tgcctactgg ctgaccaaag gggatagcgt
    30421 gggtatagcc tgcctgctgg catgcgcgat cggcgatccc gcgactgaca taacgatcgg
    30481 tacgggcatg ctgcaaaaaa gcctgatcat aaaccaaccg aacaatacct gttgccgcgt
    30541 caacttcgct cacatgggcc tggcgagtaa ccgtacaccc cgtcagcgtt attaaggaaa
    30601 gagtcagaat cagaagtctc atggtccagc cagaagagta aaagaaagca gtattttacg
    30661 cgttacagcg ctgcgacaat ggccggaaaa gacgcctggc aggcgctatt tcttgccatc
    30721 tgtatgtgtc agaaattgga gggctgcttt tttgattatc atttttataa cattaaaggg
    30781 gtatttatgt gatttacatc acataaagat tgatttttgt gatggttgtc atattataaa
    30841 cgcaaagaca ataacacctg tataacaaat ggtcggagta ccgcgatgaa actgcgcaag
    30901 atcctgaaaa gtatgttcaa taactattgc aagacgttca aagacgtacc gccaggcaat
    30961 atgttccgat aacaaaaaac ctgctccggc aggttttttt gtgttctgat gacggtggat
    31021 gtgactatcg ctgaagatgt gaagccgcta ctataacggc tttcaaaatg ctatcaggag
    31081 tttacgatgt cccagccgct gaatgccgat caggaactgg tttctgatgt ggttgcttgc
    31141 cagttggtta tcaaacaaat tctggatgtt ctcgatgtga tcgcgcccgt tgaagttcgc
    31201 gaaaaaatgt ccagccagct gaagaatatc gactttacta accatcccgc cgccgcagac
    31261 ccggtcacca tgcgggcaat ccaaaaagcc atcgcgttga ttgaactcaa gtttacccca
    31321 cagggcgaat cccattaaaa aaccgctgct tcaaaaaaat gaggcagcgg tttctcaaaa
    31381 acttcatcgg cgcaatgcat tagcgccgat gatgatcaat gtagctgatg cgacacgaca
    31441 ttcagaacat gttggtcggt ctgcacagga cacataccag caagtttcgc actgcgcact
    31501 tcatcaagaa tggtttgcag taacaggcca tcatgctgct gttctttttc cagatcgcgc
    31561 aggaaattga cggttgaatc atcattcagt tctttcgctt catcggctaa ctgtgccagc
    31621 gtgcttgaac gttgctcgta ttcttccatc gttttttgga acagttcttc cagagagttc
    31681 agtttttcac cgggaacatc aatggcttta acgatggggg tagcgccgac actcttcata
    31741 aagttaaaca tgcgcatcat ttgggtcaca ttactctgtg cctgggcgcg aaggaaagtg
    31801 gcggtgccgt tcagactctg ttcagaacac cagttactca ggtgaaggta gagattggat
    31861 gcgtaaaact cgcggttcat ttgagagttg agtttgagaa gcattccagc ggttgccata
    31921 cctaatatcc ttatatccag aagtggagag gtgcaagata aatcaaaaca ccataattac
    31981 tgtttgcaga ataattcctt tctg
//
LOCUS       DACADA010000040        30934 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.040, whole genome shotgun sequence.
ACCESSION   DACADA010000040 DACADA010000000
VERSION     DACADA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.040"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            440..2071
                     /gene="oppA"
                     /locus_tag="ISB17_001833"
     CDS             440..2071
                     /gene="oppA"
                     /locus_tag="ISB17_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAP1979773.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2157..3077
                     /gene="oppB"
                     /locus_tag="ISB17_001834"
     CDS             2157..3077
                     /gene="oppB"
                     /locus_tag="ISB17_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAP1979774.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3092..4000
                     /gene="oppC"
                     /locus_tag="ISB17_001835"
     CDS             3092..4000
                     /gene="oppC"
                     /locus_tag="ISB17_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAP1979775.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4012..5025
                     /gene="oppD"
                     /locus_tag="ISB17_001836"
     CDS             4012..5025
                     /gene="oppD"
                     /locus_tag="ISB17_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAP1979776.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5022..6026
                     /gene="oppF"
                     /locus_tag="ISB17_001837"
     CDS             5022..6026
                     /gene="oppF"
                     /locus_tag="ISB17_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAP1979777.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6079..6408)
                     /locus_tag="ISB17_001838"
     CDS             complement(6079..6408)
                     /locus_tag="ISB17_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAP1979778.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6443..7903)
                     /gene="cls"
                     /locus_tag="ISB17_001839"
     CDS             complement(6443..7903)
                     /gene="cls"
                     /locus_tag="ISB17_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP1979779.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8046..8219
                     /locus_tag="ISB17_001840"
     CDS             8046..8219
                     /locus_tag="ISB17_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAP1979780.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8274..9527)
                     /gene="kch"
                     /locus_tag="ISB17_001841"
     CDS             complement(8274..9527)
                     /gene="kch"
                     /locus_tag="ISB17_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAP1979781.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9827..10123)
                     /locus_tag="ISB17_001842"
     CDS             complement(9827..10123)
                     /locus_tag="ISB17_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAP1979782.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10347..11063
                     /gene="tonB"
                     /locus_tag="ISB17_001843"
     CDS             10347..11063
                     /gene="tonB"
                     /locus_tag="ISB17_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAP1979783.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(11103..11501)
                     /gene="yciA"
                     /locus_tag="ISB17_001844"
     CDS             complement(11103..11501)
                     /gene="yciA"
                     /locus_tag="ISB17_001844"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAP1979784.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11606..12145)
                     /locus_tag="ISB17_001845"
     CDS             complement(11606..12145)
                     /locus_tag="ISB17_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAP1979785.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12175..12918)
                     /locus_tag="ISB17_001846"
     CDS             complement(12175..12918)
                     /locus_tag="ISB17_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAP1979786.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12946..13035)
                     /locus_tag="ISB17_001847"
                     /pseudo
     CDS             complement(12946..13035)
                     /locus_tag="ISB17_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13274..13912
                     /gene="ompW"
                     /locus_tag="ISB17_001848"
     CDS             13274..13912
                     /gene="ompW"
                     /locus_tag="ISB17_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAP1979787.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            complement(13958..15088)
                     /locus_tag="ISB17_001849"
     CDS             complement(13958..15088)
                     /locus_tag="ISB17_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1979788.1"
                     /translation="MARPRKYKTNVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(15066..15314)
                     /locus_tag="ISB17_001850"
     CDS             complement(15066..15314)
                     /locus_tag="ISB17_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP1979789.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(15379..17850)
                     /locus_tag="ISB17_001851"
     CDS             complement(15379..17850)
                     /locus_tag="ISB17_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP1979790.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQ
                     PDLVKVTADEVNKIMQAASISQPDANKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     HQNQSETEQYDQKAEQNSQNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCG
                     AVMGDATYQETFNEESQDEAREKDPEEIENAGLPNKECTEGDQHANDNNEIGETANPL
                     IKVNSHRKSTSTSRLWHHLMIDLETMGKNPDAPIISIDAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFENTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            complement(17943..18134)
                     /locus_tag="ISB17_001852"
     CDS             complement(17943..18134)
                     /locus_tag="ISB17_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HAP1979791.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(18131..18319)
                     /locus_tag="ISB17_001853"
     CDS             complement(18131..18319)
                     /locus_tag="ISB17_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HAP1979792.1"
                     /translation="METLLPNVNTSEGCFEIGVNISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            complement(18885..19103)
                     /locus_tag="ISB17_001854"
     CDS             complement(18885..19103)
                     /locus_tag="ISB17_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979793.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARTKQAE
                     NLSALEQDITRLEKAAALSIARMVTYPR"
     gene            complement(19263..19418)
                     /locus_tag="ISB17_001855"
     CDS             complement(19263..19418)
                     /locus_tag="ISB17_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAP1979794.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(19691..20407)
                     /locus_tag="ISB17_001856"
     CDS             complement(19691..20407)
                     /locus_tag="ISB17_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979795.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            20457..20672
                     /locus_tag="ISB17_001857"
     CDS             20457..20672
                     /locus_tag="ISB17_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1979796.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            20669..21094
                     /locus_tag="ISB17_001858"
     CDS             20669..21094
                     /locus_tag="ISB17_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP1979797.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            21117..22079
                     /locus_tag="ISB17_001859"
     CDS             21117..22079
                     /locus_tag="ISB17_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979798.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            22086..22832
                     /locus_tag="ISB17_001860"
     CDS             22086..22832
                     /locus_tag="ISB17_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1979799.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            22854..23624
                     /locus_tag="ISB17_001861"
     CDS             22854..23624
                     /locus_tag="ISB17_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAP1979800.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            23640..24065
                     /locus_tag="ISB17_001862"
     CDS             23640..24065
                     /locus_tag="ISB17_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP1979801.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            24240..24905
                     /locus_tag="ISB17_001863"
     CDS             24240..24905
                     /locus_tag="ISB17_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP1979802.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            25086..25298
                     /locus_tag="ISB17_001864"
     CDS             25086..25298
                     /locus_tag="ISB17_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1979803.1"
                     /translation="MSHKCRLASYVPKGKGKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRLRTGQTEVAVFTAYEPEE"
     gene            25466..25738
                     /locus_tag="ISB17_001865"
     CDS             25466..25738
                     /locus_tag="ISB17_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979804.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            25740..26795
                     /locus_tag="ISB17_001866"
     CDS             25740..26795
                     /locus_tag="ISB17_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP1979805.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            26796..27176
                     /locus_tag="ISB17_001867"
     CDS             26796..27176
                     /locus_tag="ISB17_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP1979806.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMHEEQVKK"
     gene            27173..27994
                     /locus_tag="ISB17_001868"
     CDS             27173..27994
                     /locus_tag="ISB17_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1979807.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCRGEKFIEGEVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKIREVVKVSCPECKGKGEISTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            28221..28418
                     /locus_tag="ISB17_001869"
     CDS             28221..28418
                     /locus_tag="ISB17_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP1979808.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATAGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            28570..29619
                     /locus_tag="ISB17_001870"
     CDS             28570..29619
                     /locus_tag="ISB17_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAP1979809.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQGLIV"
     gene            29669..29744
                     /locus_tag="ISB17_001871"
     tRNA            29669..29744
                     /locus_tag="ISB17_001871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29702..29704,aa:Ile,seq:cat)
     gene            29845..29921
                     /locus_tag="ISB17_001872"
     tRNA            29845..29921
                     /locus_tag="ISB17_001872"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29879..29881,aa:Arg,seq:tct)
     gene            30421..30552
                     /locus_tag="ISB17_001873"
     CDS             30421..30552
                     /locus_tag="ISB17_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAP1979810.1"
                     /translation="MIAYCFCEWHCCSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT         7923 a         7188 c         7875 g         7948 t
ORIGIN      
        1 gtaatcagaa aagagagaca tgcttaacta acctctattt gattgaattg aaagatgttt
       61 gttaaggcat ggatgcaagc tatagattct ggtacggtca ataaaagaga attgcttaac
      121 aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca
      181 ggcgattctt tattggtaat gagaataatt aacaattaaa gaccgtcgcg aaagaataat
      241 gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga
      301 ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc agaaagtctc
      361 cgagcctgtg cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa
      421 tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt
      481 tttggctgcg cttatggcag ggaatgtcgc gctggcagct gatgtacccg caggtgtcac
      541 actggcggaa aaacaaacgc tggtacgtaa caatggttca gaagttcagt cattagatcc
      601 gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact
      661 ggttagcgat cttgacggcc atccggcacc tggcgtcgct gaatcctggg ataataaaga
      721 cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt
      781 cacagcacaa gactttgtgt atagctggca acgttctgtt gatccaaaca ctgcttctcc
      841 gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa
      901 aaaaccgatt accgatctcg gcgtgaaagc tattgatgac cacacattag aagtcacctt
      961 aagtgaacct gttccgtact tctataaatt acttgttcat ccatcaactt caccggtgcc
     1021 aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa
     1081 cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc
     1141 gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc ctattgcttc
     1201 tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgactt ataacaacat
     1261 gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc
     1321 atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattta atgacgtgcg
     1381 tgtgcgtacc gcactgaaat tgggtatgga ccgcgatatc attgttaata aagtgaaagc
     1441 gcagggcgac atgcccgcct atggttacac tccaccgtat actgatggcg caaaattgac
     1501 tcagccggaa tggtttggct ggagccagga aaaacgtaac gaagaagcga aaaaactgct
     1561 ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgttgt ataacacctc
     1621 cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt
     1681 aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggcac
     1741 ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt ccttcctgaa
     1801 caccatgctg tcgaacagct caatgaatac cgcgcattat aagagcccgg cctttgacag
     1861 cattatggcg gaaacgctga aagtgactga cgaggcgcag cgtgcagctc tgtacactaa
     1921 agcagaacaa cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc
     1981 gcgtttggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg ataataccta
     2041 cacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc
     2101 tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt
     2161 taaaatttat tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta
     2221 tttcgttctt tatgatgcgc ctcgcgccgg gcagtccttt taccggcgaa cgtactttac
     2281 caccggaagt gatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac
     2341 agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg tttaaatata
     2401 aagattattc ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc aaactgggag
     2461 ctgcagcatt ttttcttgcg gtaatactgg gtgttagtgc tggcgttatt gccgcattaa
     2521 agcaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc
     2581 ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc
     2641 cgggcggtgg ctggaatggt ggagcgctta aattcatgat attgccaatg gtggcgttat
     2701 cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt gaggttttac
     2761 actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac
     2821 gccacgcatt aaaacctgct ctgttacccg tgctctccta tatggggccc gcatttgtcg
     2881 gcattattac cggttcgatg gtcatcgaaa ccatttatgg tttaccgggg attgggcaat
     2941 tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagtctg accattctgg
     3001 ttggcgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc
     3061 cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac
     3121 gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg
     3181 tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc
     3241 gttatttgta atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc
     3301 gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg actcatccgg
     3361 tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc
     3421 tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg gttatctggg
     3481 cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat
     3541 gttcttcgtc attttgctgg tgaccttttt cggtcaaaac atcctgctga ttttcgtggc
     3601 gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct
     3661 gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc cgggcattgt
     3721 tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctatgcgt cgcttctggt
     3781 gcccagcatg atcctctttg aatctttcct tagcttcctg ggattaggta cacaagagcc
     3841 gttaagcagc tggggtgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg
     3901 gttactgttg ttcccagcgg gattcctcgt ggtaacgctg ttttgtttca actttatcgg
     3961 cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta
     4021 attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa
     4081 gatttgcgtg tcacctttag taccccggat ggcgacgtca cggcggtcaa tgatttgaat
     4141 ttttcgctac gtgccggaga aacgctgggc atcgtaggtg agtctggttc gggcaaatcg
     4201 caaaccgcat tcgcgttgat gggcctgtta gctgccaacg gacgtattgg cggatcggca
     4261 accttcaatg ggcgtgaaat cctcaatttg ccagagcatg aactcaataa actgcgagct
     4321 gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc
     4381 ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt
     4441 gaagaatcgg tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa
     4501 atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc gatggcattg
     4561 ctatgtcgac ctaagctgct gattgcggat gaacccacta ctgcgctgga cgtcaccgta
     4621 caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac cgccatcatt
     4681 atgattaccc acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac
     4741 gccgggcgca cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgtacatcct
     4801 tattctatcg gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga aacaatgttg
     4861 actattccgg gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca
     4921 cgttgtccgc atgcgatgga tatttgtagt agcgcaccgc cgctggaaga gtttacgcct
     4981 ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag
     5041 gaagaaaagt cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca
     5101 aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgta actcttcgcc
     5161 tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg
     5221 ctcgcgccat cattggcctg gttaaagcga ccgacggtca tgttgcctgg ttaggtaaag
     5281 agttgctggg catgaagccc gatgaatggc gtgccgtacg cagtgatatt cagatgattt
     5341 tccaggatcc gttggcatcg ctgaacccgc gtatgaccat cggcgagatc atcgctgaac
     5401 cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga
     5461 tgatgctgaa agtcggtttg ttgcctaacc tgattaaccg ctatccgcat gagttctccg
     5521 gtgggcagtg ccagcgtatc gggattgctc gcgctcttat tcttgaaccg aagctgatta
     5581 tctgtgatga gccggtgtcg gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc
     5641 tccagcagtt gcaacgtgag atgggattgt cattaatttt tatcgctcac gacctggccg
     5701 tggtaaaaca catttccgat cgtgtgctgg tgatgtatct cggccatgcg gtagaactgg
     5761 ggacctatga tgaggtctac cacaatccgc tacatcctta caccagggca ttgatgtcgg
     5821 cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg
     5881 aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt tgtccgattg
     5941 caggtccgga gtgcgccaaa acacgtcctg tgctggaagg gagtttcaga cacgccgttt
     6001 cttgcctgaa agtcgatccg ctttaaaagc aataagggct gacagttgtc agcccttatt
     6061 cacgccaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt gatctttttc
     6121 gcggcataac agaatgcgcg cgaaaacatc gttgatttca ccatcttcac tgtccgccag
     6181 accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat gttcctgcca
     6241 gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa gattgaacag
     6301 cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat cgtaggcctg
     6361 ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag tcactacctg
     6421 tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac agtcgctcgg
     6481 cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga cgagaacgcg
     6541 aaatataatc gtcctgaacg gcggcgaggt ctgcaccaaa acctttatcg tcgattgcca
     6601 gggtaatctc gaaatttagc cacagactac gcatatcaag gttaacggtg ccaaccagac
     6661 ttagttctcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca aactgataaa
     6721 ttttaacccc agcagccagc agttctgtaa agaatgcgcg actggcccag ccgaccagca
     6781 tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc gccgccgtgc
     6841 aaatcgcatg aagtaaatca tcgcttggca caaaataggg cgttgtcatg atcaaatatt
     6901 cacgagccga ataagcagca gtcaataatg cctggtgaat gagatcttcc ggaaagccgg
     6961 ggccagaagc aattgtgtga atggtgtgac cactggcctg ctcaaacggc ataatattga
     7021 catctggtgg cggcggcaga atacgtttcc cggtttcaat ctcccagtcg caggaataaa
     7081 taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca atccattgcc
     7141 ctacacccgc atcttgtttg aagtagcgag ggtcgaccat attcatgctg ccggtgtacg
     7201 cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata cggcgtaaaa
     7261 acacacgcat cagattgacc tttaaggctt cgacaacttc aataccggca ttacgcatta
     7321 gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc atcaatcggc
     7381 aatgaatacc gcgccgtgca gcggccatta atgattcagc cacctggtcc gccatgccgc
     7441 cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg atgtcgcgga
     7501 ttaacgcctg catcacatca tctgactcgg tcatcagttg tagctgattc cctttgaccc
     7561 cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca ctgctatttt
     7621 cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg gtggaaggcc
     7681 acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca acggcaagat
     7741 aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc atcgcggagg
     7801 gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc aaccagtatc
     7861 ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag aaatcctttt
     7921 gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa aacgagggaa
     7981 aagcactggt cagcgtcagg ggcgggggta taatgaccat tctgttgttg catagagtag
     8041 ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt caggctagcc
     8101 gacgtaaatc gcgttggctt gaggggcagt cgcgccgaaa tatgcgtatc cacagcatca
     8161 ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac aatatctaaa
     8221 tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa actctatttt
     8281 tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct gaaaatgcca
     8341 tgaccggagt tcaacaacat tgaaacgagc atatcattat taatctcttc accatttaaa
     8401 actcgcgcca gaatttcgct gccaaacagt tgtggtgaga gaatgatatc cggatgtacc
     8461 atcttaatct tattcaggtt tttgctatca ctgacggcaa gaactgtttt gacatcactg
     8521 ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc actcagcgcc
     8581 agaatggccc ggcaacgatc gattcccgct ttctttaaca ctgaactgtc attactgtca
     8641 ccggggataa catcggcgtt atcgcctaag cgttgctcaa gttgcttgat atcatcttca
     8701 ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag aatcgtattg
     8761 atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat tgtatgattg
     8821 tttcctttta caagtttgtt gaatcccccg cggataagcg gaccaaaaat tgaggtcata
     8881 gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa tcgtgctgat
     8941 tcagaaacag ggacaatatc gccatagccg acggttgaca tggtttctat cgaaaaatag
     9001 aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata aagcgcaccg
     9061 taagtggagt aaaacagtaa cgtcgtgaaa ctaataaatg cgaaaattgt cccggctgct
     9121 gcgctactgt gggagaagtc tttgctcagt atcagcaaaa acaccagcgt aaaaatgcaa
     9181 aatccaatac taaatttcag ccagggataa aaatgcagag tgtaaattag cgctatcaac
     9241 aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc aatggagttc
     9301 aggacaagaa atacgccgag cataaatagt ggggcgtttg ccagcaagga gagatccagc
     9361 tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc atagattgtt
     9421 ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg ccgtagggtc
     9481 acccataact ttgttgctgt ttgtttgaat gtagtccagt gactcactga tacctctgta
     9541 accacaattt tgtttaatta tctcaacgtg cacattttct gaacatacat gcagctcgtg
     9601 aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt gagtgagcaa
     9661 ctctgcgtca ggaaagggca ttttcctgaa tatgttcaca tcatataaag aaatacagat
     9721 aaagttatta tctgcttgtg gtggtgaatg cactgaccga cctataagga aaggccaaac
     9781 aagaacacgg ttgcaaaaac cgtgccctta aatattgaat ttctattcag aacactttct
     9841 taaatggttt cactgaaacg tgttcataga ctcctgccgc tacgtacggg tcagcatcgg
     9901 cccaggcctg agctgcttcc agcgactcaa attcagcaat aacggttgag ccagtaaatc
     9961 ccgcagcccc tggatcgtta ctgtctaccg ctggcattgg accggcggtc aacaaacgac
    10021 cttcatcatg cagtaactgt aaacgtgcta gatgtgccgg acgaacggaa aggcgttttt
    10081 cgaggctatc agctttgtct tgagcgtaaa taacatacaa cacaggcaca actccttgtt
    10141 cgggaaagtt gtaaagtacg ttatttgaaa gggcgaagat ctgcaacgga aagatgatgt
    10201 ctttgttaag accatgcata aagtaaggtc aattacgcca aaaatgacat tttcactgat
    10261 cctgatcgtc ttgccttatt gaatatgatt gctatttgca tttaaaatcg gggcctggtt
    10321 tttctactga aatgattatg acttcaatga cccttgattt acctcgccgc ttcccctggc
    10381 cgacgttact ttcggtctgc attcatggtg ctgttgtggc gggtttgctc tatacctcgg
    10441 tacatcaggt tattgaacta cctgcgcctg cgcagccgat ttctgtcacg atggttgcgc
    10501 ctgctgatct tgaaccgcca caagccgttc aaccaccacc ggagccggtg gtagagccag
    10561 aaccggaacc tgagccgatc cctgaaccgc caaaagaagc tccggtggtc attgaaaagc
    10621 cgaagccgaa accaaagcca aaaccgaagc cggtgaaaaa ggtggagcag ccaaaacgcg
    10681 atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcgcgcc
    10741 cgacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac
    10801 cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg
    10861 aagggcaggt taaagttaaa tttgatgtca cgccagatgg tcgcgtggat aacgtacaaa
    10921 tcctctcagc caagcctgcg aacatgtttg agcgtgaagt gaaaaatgcg atgcgcagat
    10981 ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta
    11041 acggcactac cgaaattcag taagcataaa gtcaaaagcc tccgaccgga ggcttttgat
    11101 tattactcaa cgggtaaggc gcgaggtttt ccttcgggat caaccgcgac atacttaaat
    11161 aatgcttctg ttgctttata gcgttgcccg attggttctg acgccacttt tttcacccac
    11221 acttcaatat taatgctgac tgatgtcgtc cctttctgga cacagcgtgc atagcagcac
    11281 accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacggtcact
    11341 acacgaccgt gggcaatttc tttcgccaga atagcgccgc caatatccat ttgtgacatt
    11401 aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt
    11461 aaaacaagat cgccctgagg gacgttatgt gctgtagaca tggtaaaacc gacttaaaag
    11521 gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc
    11581 cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga
    11641 tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa
    11701 agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc aggccgcaaa
    11761 ggataaagaa aacagcccag gccagattca gcttcgacca taccgattgc ggcagggtga
    11821 gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattga ctgactaaca
    11881 gggcacccgc aaacagggca taaatgacag taaccttcca tttaataaac tcatcattgt
    11941 gaaagaacag cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct
    12001 tctcaacctt acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca
    12061 gcgccgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg
    12121 gtaaaaaatc aagaaactgc ttcattttat gattccgtaa tcaaatgctt aaggttattg
    12181 gcgaatcaac atatacaggc ggaacagata gatgagcaat acggctgaaa tcaggttgct
    12241 caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa
    12301 aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat
    12361 attcgcccat gtcagccgca tactgctacg catcgaggca aaaacgccca ttttgtcctg
    12421 taccagcatc accggtgcca gagccagtaa aatggccatg ataattcccg gaacgaccac
    12481 cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataactttgg
    12541 caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac
    12601 cagctggata attaatatta cgcccccggc gagaatggcg ttaccgatta atcctgaaaa
    12661 agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac
    12721 caggtcgaac aacccactac tgccgctaac gggtacgccg tcattgagct gcgcaagctg
    12781 tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga
    12841 taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga
    12901 ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa
    12961 ttgtaccctg gttaagcccc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt
    13021 gtaaagcgga ggtaattgct ggccttcagg cgtgggtaaa ccgctgcaag tggcgagcga
    13081 gttaatattt gcgtagcgaa atatataaaa attgatttaa atcacattaa ccaggatttt
    13141 caatgcaact tctaaattaa tctagatcaa taaagggtga attaccatat gtaatgtgat
    13201 ctgtgtagga tcatttgtta ccccaatgtg ggtatattcg tcacgttttt ataaccataa
    13261 cgacggagcg gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctctc
    13321 tggcagtgcc tttgcgcatg aagcaggcga attttttatg cgtgctggtt ctgcaaccgt
    13381 acgtccaaca gaaggtgctg gtggtacgtt aggaagtttg ggtggattca gcgtgaccaa
    13441 taacactcaa ctgggcctga cgtttactta tatggcgacc gacaacattg gtgtggaatt
    13501 actggcggca tcgccgtttc gtcataaagt tggcacagcc acaaccgggg atattgcgac
    13561 agtccatcat ttaccgccaa cactgatggc acaatggtat tttggcgatt ctggcagtaa
    13621 attccgtcct tacattggtg cgggtattaa ctacaccacc ttttttaata atgacttcaa
    13681 tgacaccggt gaaggtcttg gtttatctga ccttagtctg aaggattcat ggggcgtggc
    13741 ggggcaagta ggcctggatt atctcatcaa ccgtgactgg ttgctgaata tgtcagtctg
    13801 gtacatggat atcgatacca cggctaagta caaatctggt gttactaccg taaaagactc
    13861 ggtacgactg gatccgtggg tgtttatgtt ctccgcagga tatcgttttt aattttctct
    13921 gcaaaacctc tgcaaaaccc ctctgcaaaa ctggtcatca aatgaccagt tttttccatt
    13981 ccttaccgcg tgcgtcgttg taaatatcgg tcattttttg attcgaatgg cccagcaaaa
    14041 ttttggtatc aaccccctgc tctctgaaca atcgctctga taaagatctc tgctcatgga
    14101 aagagggtgg ggtgccatta gcacgccagt tgtaatccac agaatcccgg gcttttttaa
    14161 atgcaacggt taatgttgct ggcttaacca tcccgccgcg cttagctgtc cctttcgcgt
    14221 gatggtggtg caatagccac ggactaagaa cgcaatcgcg gcaggatgac accacatcat
    14281 ctagggtgag atttaattta tcgcaacgca gagccagagg gatggcaatc cgggttcctg
    14341 ttttttgctg ttcgacatga agataaccat cccggatatc cgaaaattgc attttgcaaa
    14401 tatctgaaag gcgctggcct gtcatcagtg ccagcagcat accgcgctgt aaaaagtaac
    14461 catccttttc cgctgcgtta taaatcatca tccactcatc aaaagtcagt cgctgtcttg
    14521 atatccgcac ctgcggtttt tttgccgatt ctgcagggtt aaagcctggc gggacatcgc
    14581 ccgtttgctg agcttcccgg aaaacatcga tcagtacctt cctgaaaatt tgtcccattc
    14641 tgttatgtcc tctggccttg tactcttcca gtactgatac cacatctttt acggttatgg
    14701 catctaacgg tctggtgcca aaacgttcat caaataccct gagaggggcc gctttctgtt
    14761 tcagcgtgtt gagtttgatc tcgccgtttt catatctttc ctgttgaatt tttctgtaat
    14821 tattcagaaa aatggtaacg gttgatgaac cgccggtatc actaataatt ttctcctgca
    14881 gactgagcat ttgttccatt tgctgccggg caagacggct gttcgcttct gctgcaatag
    14941 tttctgccag tttctggtca atactgccga gaccgtgatt tttgcctgtt atgggatgcc
    15001 tgtaacgcca gtaaactttg ttatttcttt tgtcaaaata cggagataat cccggaacat
    15061 tggttttata ttttcgcggg cgcgccatct tccagtatcc tcttcaaagc agggtgatct
    15121 gtggcgatca cctccggctt gtttaccatt ccgacaaagc gagcttgcgg atccactcgc
    15181 cagcgtcttc caactttttt ggggagagga aatatcattc cggctttagc gtatttactt
    15241 aacgtactcg gagttgggac cggttcactg aattcctctt ttgcccactc agtgagcaga
    15301 ataagtcttg ccatgagcgt cgttcgctaa tcatggtcgc cgccactata gctggtgggc
    15361 aacgaccggg gttgaacatt aaaaatcagc ctgattcggg atcagttttt gccagataac
    15421 tgaaacgtat tttgcctggt aacgggcgtc atcaagtgca ttatggcgct caccttcgaa
    15481 tggaatagcc gttctggcat cgaagtctat ggctttcccc agctcaacga ttgtgcgtac
    15541 atcgcgatcg ttgtagtaac gccatgggca ggggatcccc tgccgttcgt atgaacggcg
    15601 caaaatcgtg ttttcgaagt tggctccatt tccccagacc tgaacaaaaa attcaccgga
    15661 gttttcgtcg ataaattccc gcaattgtaa cagtgcatca tctaacggga tttcatcggt
    15721 cataatggca gactgcgctt cacgtgattg cttcagccac cttttaatga cgtcacgatc
    15781 aatgactccg ccagcagttt ccagatcgat agtcttacta aattccggtc ccatatctcc
    15841 ggtttgcgga tcgaaaaata ttgcatctat tgagataatc ggggcatcag gattttttcc
    15901 catggtttca aggtcaatca tcagatggtg ccacaacctg ctggtggatg tgcttttacg
    15961 atgactgttc accttaatta agggatttgc tgtctcgcct atttcattat tgtcattggc
    16021 atgttgatcg ccttcggtgc actccttgtt cgggagtccg gcattttcta tttcctctgg
    16081 atctttttcc cgggcttcat cctggctttc ttcgttgaat gtctcctggt aggttgcgtc
    16141 acccatcact gcgccacaat cagggcagtt accaccacca gtctgattgc atgcggtaca
    16201 aactttttcc tcttcctgtt gcacgactgg ttcaggttgt ttcgtttctg gctcgttttg
    16261 taacgcattt tggctgtttt gttccgcttt ttggtcgtac tgttccgttt ctgactggtt
    16321 ctggtgcaca gaatcgcggg tttcaatccc cttcacccat ttcggatcat tcgggtcgct
    16381 aatcccttca acaaattcac cacgtgatgc agcgagcaac ttattagcgt caggctggct
    16441 gatactggct gcctgcataa ttttgtttac ttcgtcagcg gtaactttta ccaggtctgg
    16501 ttgtgcggtc gtgtcagatg catcagtatt ttgttgtgaa cctgtgtatg tgccgttttt
    16561 gcgggcaaaa tattcttctt tcgtgatttc agttgccccg gcagccagcg ccttatccag
    16621 accagaaagt ttgtttgcgc gaccgtattt ttcgccatcc ttgtcggtga agaggaagta
    16681 gaacggcccc tcacgctcta cagatggttc gacttccact ttgcattcgg ttttttcgtt
    16741 gtccggaatt gccgtttcca ctgcatcagt ttctggtact ggcgacgaga gagtatcagt
    16801 tgcgctctga tttcttcctt catcttcaaa cacgcccttt gtagtcaggt attcagtaat
    16861 gtatttgttc agtgccacag ggtctttgtg aatgtcgatc ggacgttcac ggacaaggcc
    16921 aaaaatagtc tggcggtcgt agcgaagggc atcaggctgt ttgcgcattg atgccgagat
    16981 acgcttccag tcttcgcggt cgttgtcgat aacttcattt tttgcccagc gatggatgct
    17041 gccgtcaatg tttccggcat ccacatcacc aggccagaga gcgtaggcca gttcgtcatc
    17101 cagtgttttc catgtctgct tgtattcgcg atgaatggca gcaatgaccg ggctgatttt
    17161 tcctgttgaa ttttcagtgt actgttgatt ggctctggcg cgggcgagat caacaacaga
    17221 cgtgtatttt ccggtttcct tgcgttcacc ttcacgacgt tttttccaga tgcgcatctc
    17281 tgcctgaatt tcgggccatt tggcaccagg cttacattta tgcttaaccc acccgatggc
    17341 atgcagctta agctccggat acatggcgtt aacttctggc attttcatca acgcttcaac
    17401 gatatgtccg tcgaatgttg ccatgtcttc ctgcaacaat tcctgtgcgc taataaccat
    17461 atcaacggtg atgttttcac atgtgtcgaa cttaaccatg acagcgttct gtacttcagg
    17521 ggccagcttg tcaaaagtga cgttcatcgg atcggattca gtctcaaccg ggacaaagga
    17581 agcagactcc tcatcccagc ggttttcctg catatattca gcatcccagg aatcgagggc
    17641 agggcggggt ataccgggtt tatcctcgca gacaagaaat ttataagcgc agtcctgagc
    17701 agccggataa tgttccagga attgccagtg aaattttgcg cgggcgcgac gttcatcacc
    17761 ggcttcaatg gcagtggcta cagcgacggc accttcttcc tttattgcct gttcgtccgg
    17821 aatggcggcg caaataaaga ctttactcat tttgttttac ctcattacag atttaagggt
    17881 gaacaaatcc ctgccattgc tggcatataa gaatgaaacc ggatatttat tacggaactg
    17941 ttttaaagac ctgccgggat ttcgttatta tcctggtgaa taactttatc gaccgggtaa
    18001 cagttaccgg gaattttctg ttcggttgct gcagtcacac actcctgcat tgtcctgtga
    18061 acactgactg caatatcaac tggctctccg gaaacaagaa aaactgtcag aacaagtgca
    18121 aatgctgtat tcattgccag catccttttt gtatcggacg taaacgggcc agcattgaaa
    18181 gaatgcatat tttatttaat agctcccgtt cgtgttttct cttgttaatg gcatcttcag
    18241 taaatacagg gttactgata ttgacaccaa tttcaaaaca accttcagac gtattaacgt
    18301 ttggtaataa cgtttccatt atcgcgtcct caacaatgaa ttttgtgatg cggtgcctgg
    18361 tgcctccagg tgacgttaac cagttaacaa ttaacgccgg atacagagaa tccacccata
    18421 acactgtttt tggttttaac tgttccgcgt gcgctcagcc gcattcacca catcacaaaa
    18481 ttcactttaa aaagggcggc agagcagtca cggagtaaaa ctgataccgc caaacgtcac
    18541 cagaaaattg ataacagagg gcgttgcagc ggagttgtca cttaagcgta tggtcaacct
    18601 gacaacccgg tgtcctcaac ggaggaagga ataaccccgc catacttacc gccgcgccat
    18661 ttcgcggagt gccacaaccg gaagcgcacg gtcgaactaa atttaacgac accgtacaga
    18721 gagaccaatt tcgccgtgcg ctttcgcttt atgccctgac ttttcaggga catatccttt
    18781 cagtaaactg tcagtgccgg atgttcaccc gtgtccggcg cacgcactcc acctgacccg
    18841 tggagaactc cttaattacc aaccttagct tcgttggtta gctattaacg cgggtatgta
    18901 accattctgg caatgcttaa tgccgctgct ttttccagcc tggtgatatc ctgctccaga
    18961 gcggacagat tttcagcctg cttagtcctg gcttcattgg cccatttcag atcctgcgct
    19021 gcattaattt tctggcgcat ccactcataa agttcatcat cggtatagtc tggcgcgatg
    19081 atgacgggtt ctcgtttctg catactgatt cctcgcggtg ctgcttcgct tatcagccgt
    19141 tagattttgc cgagctggaa agcgcctgtt taaactcact gaagctgaga gcttcttcgc
    19201 cttcggcaag gccttcgaag tattcttcgt aagccttttc catgattgtg tcgaaatcca
    19261 tatcactcac ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt
    19321 ttgcttttgg tatacgtcat tgcggtgaac gtgccgtcct ggttggggaa cacgccacat
    19381 accagagatt cgctgttgcc aagatcgata gtatccatgc tgacctcatt tccccttaac
    19441 gccgggggag cggaacaaaa acctgctgca tagttattaa agttgaaccc tgccgtcatg
    19501 ttcttacgcc tcgggctggc tacttacccc ctgaccactg cttggtaact cgaagtattg
    19561 cccggcgttc tgtggggcgg ggtgggttgg tatgttgtta aggtaacaag agttaccttt
    19621 cgagtcaata caatgttgca aaaggtacat ttgagggcat aaaaaacccg caatgaatgc
    19681 gggttctgac tcagtctaag tattgatgta tttgtgaaac tttaccttta atggtgtaac
    19741 caccattcag ttcgatgggt ttgtaaagcg gattcagtga caacagatag atgtttggtc
    19801 cgtcaatcgc aacttttttt agtgttacgt ttggcgttcc ttccaattgg attaagatta
    19861 tttttcccac cagttctcta atgttacttg agcatggtgt gatcagcacg gtagatccgt
    19921 cggggatggt tgggaggccg ttagagtttg tcatcgcatc tccctcaaca tgcaataaaa
    19981 aagagttttc agcggttttt gtcatgacat caacccagtt cttaatacca ggaatcttgg
    20041 ttactggaca actcatatcc caataaccag cctgttccca cgttaaaacg ggcaaccggg
    20101 cgatgttgtc actaatgtaa gggtactgat tcagacgcag atcatcggtt ttatcgtgac
    20161 cgtcctttcc ataaagaatc cattcaggag atttggaaag caattttgac agtagataca
    20221 aattctcacc gtcaggtttt gaagagccat tttcccattt tgttacggat acacgagata
    20281 tgccgattgc tttcgcaacc tgctgttggg ttaatccaac gtcttttcga cgattccgaa
    20341 tacgttcgct gatagtgttt ttcatgtaac caatgttact accaagtgat gttgctatgg
    20401 ttgacattgt tatgtaacta ttgttaccct tctgctcgaa ataacaggag agttttatgt
    20461 tcaaagatga tgttctgcgc tatttcaaaa aaaagcgact agtagctgag gctcttggaa
    20521 tttcacatgt ggctgttgtg cggtggaaag cagttattcc caaacttcgc gcaatggaac
    20581 tggatgaaat tactaacggt gaattgaaat acaacccaga actttacaag aagcaggata
    20641 gcacctcgaa cgaaggaaag aatgattcat gaaaatcaag catgaacaca tccgcatggc
    20701 gatgaatgcc tgggcgcatc cggacggcga aaaagtgccg gctgcgaaaa ttaccaaagc
    20761 gtatttcgag ctgggaatga cgttcccgga actgtatgac gacagccatc cggaagccct
    20821 ggcccgtaat acccagaaaa ttttccgttg gctggataaa gacacccctg atgctgttga
    20881 aaaaatgcag gctctgttac cggcgatcga aaaggcgatg ccgcctttgc tggtggcccg
    20941 tatgcgcagc cacagttctg aatattaccg tgagatcgtc gaacggcggg atcggctggt
    21001 gaaggatgtc gatgattttg ttgcgtcagc ggttgttttg tatgaccaga tgaatcgcgg
    21061 cggcccggca gggaatgctg tggtgatgca ctaaaagcac ggtgttcggg ggttttatga
    21121 gcagcaagct tcatggtctt gtctgggaag ggtgcgcctt caccggcatg atcttatcca
    21181 gggtggcggt tatggcccgt cttgcagact acagcaatga cgagggcgtg tcatggcctg
    21241 ccattgaaac tatccggcgt cagatcggtg caagaagtga atccacagtg aaatcggcta
    21301 ttgcagaact ggcgaaagag ggctggctga cgaaggaaga gcgtaaggtc ggtgggcgta
    21361 atgtaagcaa tatctatcgg cttaatgtgg aaaaactcga agcagctgcg gcggcggcgc
    21421 gtgagtcata taaaccgaaa agaaaaatta gcccggcaaa aaatgacccg ttaacagttg
    21481 acccgtcaaa tattgacccc tcaacggttg acccgtcaaa ttttgatgga tcaactgttg
    21541 ataaaaaact gccgattagg gggccgatga ttgaccccga tccgtcagta ttaaaacctg
    21601 atccgtcaga taaaagatct tcttgtccgg acgcttcgca accggacccg cagacggctg
    21661 aacaggattt tttaacccga caccctgacg cggttgtgtt cagtgcgaaa aaacgccagt
    21721 ggggaagtca ggaagatttg gtgtgcgcac agtggatctg gggacgaatc gtgagtcttt
    21781 acgagcaggc ggccagctat gatggcgaga tcactagacc gaaagaaccc aactggacag
    21841 catgggccaa tgacgttcgc acaatgcgga tgctggatgg cagaactcac agacaaattt
    21901 gtgaaatgtt tgggcgtctc cagcgggatt cgttctgggt aaaaaacatc atgagtccgg
    21961 caaaactccg ggaaaaatgg gatgaactgg ttatccgcct ggggcgttcg cctgcgcagc
    22021 gttgcgtgaa tcacatttct gaaccggaca ctgaaatacc gccgggattc agggggtgac
    22081 gtgtcatgaa aaacattgcg gcagttgggg ttcttgaacg tattcgcaga cttgcaccac
    22141 aggggtcggt tccaccgtac cggacggtgg aggagtggcg ggaatggcaa cttgctgaag
    22201 gacgaaaacg cagcgaggag attaaccgcc agaatcgcca gttgcgggtg gaaaaaatcc
    22261 tgaatcgttc gggcatccag cctctgcaca gcaaatgctc gtttgcaaat tatcaggtgc
    22321 agaacgacgg gcaaaaatac gcgctgagcc aggccaaatc catagctgac gaactgatga
    22381 ccgggtgcac gaattttgtg ttcagcggta aaaccggcac cgggaaaaat caccttgcag
    22441 cggcgatggg caaccggctg atggtgaagg ggcgcagcgt gattatcgtc accgtgtctg
    22501 acgtcatgag cgtgttgcat gacagctacg acaacggcaa atccggggaa aaatttttac
    22561 aggagctttg cggggttgat ttgctggtcc tggatgaaat aggcgttcag cgggagacga
    22621 aaaacgagca ggtggtattg caccagataa ttgatcgccg gacagcatca ctgtgcagtg
    22681 tcgggatgtt aacaaacctg aatcatgccg caatgagtac gcttcttggt gagaggatta
    22741 tggaccgcat gaccatgaac ggtggtcgat gggtgacgtt taactgggat agctggcgtc
    22801 caaatgtcag caatatgagg gttgtgaagt aattttgtcc ggaggaaatt ttaatggaaa
    22861 ccgtatctga cgcactgaaa gcactgaaaa aagcctcttc acatgtggtg gcagctcgcc
    22921 ttggaatcag tcgtgaagag gctgtcaacg agctgtggga actcaaaaga aaaggcgtcg
    22981 ttgataaaac tggtcacacc tggtttctgg ctggcgaagg tgaatcccgg gtaaccgaag
    23041 agcggccagt aaaatctgaa gcacaggata tgctgaccgg ggaggtcgaa caaaaagtta
    23101 ccgcagacat gatgattgag tttatcggtc aggatggggc taaaacgtgt gaggaactgg
    23161 cgggtaagtt cggtgtcagt actcgcaagg ttgcttccac gctggcggtg gtaaccgcaa
    23221 cggggcggct ggcacgcgtt aatcagaacg gtaaatttcg ttactgcatg ccgggcgata
    23281 atttaccagc agagccgaaa gccgcgctgg taacggaaag tgatggtaag gcctttcctc
    23341 agccagcagg tgctgcgtta ccagtccggg aagccgcaac acaggaagaa attaaaacag
    23401 aaactgtggc ggacattgtg cagccgttgc catcgtttac cgaaacgcaa gcagatgagc
    23461 tgatttttcc gtcccttcgc agggcaaacc tggcgctgcg cagggcgaaa agtgatgttc
    23521 agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg gatattgttc
    23581 gacagattac tgattcttcc cgccgtgttg tatcggaaaa gtgattgccg gaggcgctta
    23641 tggcaaaagt atttacacaa gaagagcggg aaaaaattaa agggcaggtt gttgaacttg
    23701 tacgtctgag cggtcgcgag acgttgcggc aactggaagc caagacaggt gcgacaagat
    23761 atctgatgag tgttctcgcc agagagctgg ttgccagtgg cgatgtatac aactctggtt
    23821 acgggttatt cccgtctgaa caggcgcgta aggactggca aaatgctcgc aaaaaactct
    23881 caagggcaaa ggtgaagaaa cctgcagtgg ttgatccgga ccttatctgg tcgttaccag
    23941 acggcgaaat acgccgctac gacaggcgcc tgaatataat ctgtcgcgag tgccggaaga
    24001 gcgaagctat gcagcgtgta ctggcatttt atcaaggaaa tgttaggtat tttagacgtt
    24061 actagattaa agagcattag ttcagatgtg aattgacatt ttcatggcgc agggtagagc
    24121 cagcgtggtt gtccgctttg cgtcaaaacc agatattacc agatttagac atatattccc
    24181 gatagccctg ctctgatgct acactctgtg ctattttcat gaccccaata aaaatattta
    24241 tgactattgc tgatttcaaa cggcctaaat tggagctccc aaacggggca aacaaactac
    24301 tactgcactc ttgctgtgct ccatgttccg gtgaagtgat ggaggcgctt caggcctcgg
    24361 gaatcgacta caccatcttt ttctacaacc cgaacattca tcctcagaaa gagtatttaa
    24421 ttcgtaagga tgaaaatatt cgctttgctg aacaacacgg cgtgccgttt atcgatgctg
    24481 attacgacac cgacaactgg tttgaacgtg ccaaaggaat ggaatgggag cctgagaggg
    24541 ggatccgttg taccatgtgt tttgacatgc gttttgagcg gacagcgttg tacgctgctg
    24601 aaaatggttt cagtgtgatc agcagttcac tgggcatttc acgctggaaa aatatgcagc
    24661 aggttaacga gtgtgggcgg cgagctgttg cgcattatcc gggtatggtg tactgggatt
    24721 ataactggcg caagcagggc ggctcgtccc gtatgattga aatcagcaag cgcgaaaaat
    24781 tctatcagca ggaatattgt ggctgtgtgt attctctgcg cgataccaat ctacaccgca
    24841 aatctcaggg acgccctctt atcaaaattg gccaactcca ctacggaaaa gaagagaagg
    24901 agtgatttta tggatcacct ttctgattga tttcatattg gcgaggtgac gtgagttaag
    24961 tagaatggct gcgggtgctt gaggctatct gtctcaggca tgaacactga aaggcagata
    25021 gagaaaagcc ccagttaaca tttcgcgtcc tgcaagacgc ttaacattaa tctgaggccc
    25081 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gggaagcagg
    25141 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
    25201 tgacggcact ggtaacgagg aaagacctct gcgaggtacg actccgaacc ggccagacgg
    25261 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gacccggcgg gggagaaatc
    25321 cctcgccacc tctgatgtgt caggcatcct caacgcaccc gcacttaacc cggttcggcg
    25381 ggttttgttt ttttctggca ttctggttta caattcgcac gtcagcctga acacctgaca
    25441 cctgctgcgc cagcagagaa aacagatggc gcacaaaacc aaatttcaca attctgatac
    25501 cgaccttgcc atccggcatg ggcggcgttc acacgcattt aaaaccgact ggtaccaaca
    25561 cccaccatgt actgaagaac aggccgaatg gctaattcat aactaccgca gacgcggata
    25621 cgagattaag aaagccctca gcctcgatta tcgtcactgg ataatctccg tcaggcttcc
    25681 ttactctgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
    25741 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
    25801 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
    25861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
    25921 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
    25981 cattgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
    26041 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
    26101 gcgacaacca gctgcgcgac cagacctccg aatcactcgg gcaacttgct caccaaaacc
    26161 tgtttgcatg gatgattgac gtcatacgcc atgcaatgaa tggctcgcag gaacgggaat
    26221 tatcgctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
    26281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg
    26341 aaagcgacat cgtaccggga gagcagaccg ccaccagcat actgaagcag cgcacaaaaa
    26401 atcttgcgcc gctgcctcac gcccaccagc aaaacccgcc acaggaaaag acggtggtca
    26461 gcattgccgt tgatccggag tcaccggctc agtatctcca gcgccagaaa tcacaacggg
    26521 aagagatgcc tgtatacacg cgttgggtaa aaacgcagaa atgcatgacg tgtggcaatc
    26581 aggcagatga tccgcatcac atcattggtc atggactggg agggatggga acaaaggctg
    26641 atgatttgtt tgttattccg ctgtgccgta aatgccatag cgaactacac gccggggtaa
    26701 aagattttga agaaaaacac ggcagccagc tgttgttgct gattcgtttt ttaatgcacg
    26761 cgagaaattc gggtgttctg aagtggaaag cataaatgac tgaacgcata gaatttgttt
    26821 tgccttaccc gccaacggtg aacacttact ggcgtcgtcg tggcagcaca tattttgtat
    26881 caaaagccgg ggagcgttat cgccgggcag tggcgcttat tgttcgccag cagcggctga
    26941 aattaagcct gtccggaagg ttggcaataa aaattattgc agaaccaccg gataagcgcc
    27001 gccgtgatct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
    27061 taatggacga tgagcagttt gatgaaatca atatcgttcg tggtcagcca gtatctggtg
    27121 gacgtctggg ggtgaagatt taccccataa tgcatgaaga gcaggtcaaa aaatgaaact
    27181 ggaagattta ccgaaatact actccccaaa atcccccggc ctgactgatg catcggcctc
    27241 aacgtcgaaa gatgcgctga gtatcactga tgtgatggcc gcgcagggca tgacacagaa
    27301 tcgggctgag atggggtttt ctgcgttcct ggggaaaatg ggcatcagta tgaatgacag
    27361 ggcgcgggca acagaattac tggcagatta tgcactcagt cggtgcgatc gtgtggcggc
    27421 gttgagaaaa cttccggcag aaataaaacc ggtagtgatg cgcattatgg cttcgtacgc
    27481 ttttgaggat tatgcccgca gcgcagcgag taaaaagcag tgcccctgtt gccgagggga
    27541 aaaatttatt gaaggcgaag tttttacaaa caaggttcag tatccggatg gcaagccgcc
    27601 agtatgggca aagtgtacga aaggtgtgta tccgtcttac tgggaagaat ggaaaaaaat
    27661 tcgggaggtg gtgaaagttt cttgtcctga atgtaaaggg aagggggaga tttccactgc
    27721 ctgtaaagac tgccgtgggc gtggtgtcgc cattcatcgt gaagagtcgg taaaacgtgg
    27781 tatgcctgtt atcagagact gccagcgttg tggtggtcgt ggctgtgaaa gactaccatc
    27841 aacggaggca tttaatgcca tatgcaaagt gacgagtgct atcacgcttg atacgtggaa
    27901 aaaatcagtg aaacgctttt acgatacgtt ggtggttcgg tttgacattg aagaggcatg
    27961 ggcggagcgg cagttaaaga gggtaacgcg atagtgttgt tgatttttcc cgaatctgtg
    28021 gtaaatttgc tctaacgatg ggcgttttat gcctgacgtt agaagatttt ttacaccccg
    28081 ccgcctggcg ggttttttat gactgaaatc gcgtcagtac agtaaacgcg ctggtggcgg
    28141 tgaatacctg tctttcagct tgctggcttt ttcgacaaga gttattggtg tgtcacgtta
    28201 accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc gccagagtgg
    28261 tgtttaatga attaagcgtc accgaaccgg cgacagccgg ggagattgcg cagaatactt
    28321 acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg ggtctggcag
    28381 actatcagtt cggttgttac agacgccttc cgcagtgaag gcttttttat ttgtggtaaa
    28441 tgggcggctg gtgggtgtta ggggcaccca ccagccatct gctcatgcgt tggggtcaca
    28501 agcaaacctc aggcccatct gctttgcgca aaagcggtat gagcctatca gagaagtgct
    28561 tattgatcta tggctaatac tgtaaaaata tccagttgtg agttaatcaa cgccgactgc
    28621 ctggaattta tccggtcgtt acccgaaaat tctgttgacc tgatagtcac ggacccgccg
    28681 tactttaaag tgaagcctga gggctgggat aaccagtgga agggcgacga tgattacctg
    28741 aagtggctgg accagtgtct ggcgcagttc tggcgggtgc tgaaacctgc cggaagtctt
    28801 tacctgttct gtggtcatcg cctggcatct gatatcgaaa tcatgatgcg tgaacgcttc
    28861 agtgtgctga accatattat ctgggcgaag ccgtccggac gctggaacgg atgcaacaag
    28921 gaaagcctgc gggcgtattt ccccgccaca gagcgcattc tgttcgcgga acattatcag
    28981 gggccgtatc gtccgaaaga tgccgggtat gcggcgaagg gcagtgcact gaaacagcat
    29041 gtgatggccc cgctgatttc ttactttcgt gatgcgcgcg cggccctggg gataacggca
    29101 aaacagattg cagatgccac aggaaagaaa aacatggtgt cgcactggtt cagtgccagt
    29161 cagtggcagc taccgaacga aagcgattat ctgaaattac agtcgctgtt tgcccgggtg
    29221 gcagaagaga aacatcagcg cggtgaactg gaaaagcccc accaccagct ggtggatacg
    29281 tatacgtcac tgaaccggca gtatgtggag ctgcagagtg aatataagca tctgcggcgg
    29341 tattttggtg tgacggcgca ggtgccgtac acggatgtgt ggacacataa accggtgcag
    29401 ttctatcccg ggaaacatcc gtgcgaaaaa ccggcagaaa tgctgcagca gataatcagc
    29461 gcaagcagtc gtccgggtga cctggttgca gattttttta tgggctcagg ttcaacggta
    29521 aaagcggcac tggcgctcgg gcgtcgtgcg attggcgttg agctggagac cggacgtttt
    29581 gagcagacag tcagggaagt tcagggttta atcgtttgaa acggatgaga ttgcagaatt
    29641 aattacgcac cattattatt ctgctcccgg ccctttagct cagtggtgag agcgagcgac
    29701 tcataatcgc caggtcgctg gttcaaatcc agcaagggcc accatcacaa accgccatta
    29761 gcttatcagg aagagcagac gacacgataa cagggttgtt ggtgcggggg cgggtccccg
    29821 atggcggtcc attatcggta ttcagcgttg ttagctcagc cggacagagc aattgccttc
    29881 taagcaatcg gtcactggtt cgaatccagt acagcgcgcc atattcattc ttccagattc
    29941 cttccggcag agccttatac tgaaatatac ctggctcagg atattgttga aaatattata
    30001 tgtttgtcaa aaataaaagt tctgttaagt gttgattgag tgtttgttat acggtctaat
    30061 ggttttttca gcattaaata tttatcattc atatggtgtg ggtagagtga atattgatga
    30121 ggcgtcgggg tgtttcatcc ttaggcagcg tattgatata gtcaatgcag cacgagcaaa
    30181 ggccttcagc cgtttgacag ttttgttctg tactcctgat cgtctttcgg gaagagacgt
    30241 tattattctg aatagtgatg ctatacagag ggtttgcgat gagttcatgg tggctaattc
    30301 agaattattt actcttgttc aggagtacaa cagaatagcc aggacctgtg gtatggatga
    30361 acttcggatt actcatctgg ggtagataca tatctggatt atcaccggtt acggtaaaaa
    30421 gtgattgctt actgtttttg tgaatggcat tgctgcagcc ggataatgtc agtgctggct
    30481 gacggtgtgc tggtggcggg tgtggtggtt gttgctttcc cgttgctgaa aaagaaaacg
    30541 ccagactgtt agccgggtat cagttagcgg gagaaatttt taaatacttc acaattcagg
    30601 cggttgattg ttgtctggtt tgcggggagt ttgttaaaag aaactggcat ggtgaatccc
    30661 cctgtgcgga ggggcaatca gcgagtaggt atatgggata atcgcggatt caggtgctgg
    30721 tactgaattc accgggaggc acccggcacc atgcaatggc acatagcgcc actctccagc
    30781 ccctctccgg aggggctgtt tatattgatt ttgtcagatg tgagtaaact ccttatggac
    30841 tttgttgttt tagtccataa ggacatattt gcagagtgca acggttatta aagcattcat
    30901 tcaatacgtt atctgtattt gtagggcatt cctg
//
LOCUS       DACADA010000041        30486 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.041, whole genome shotgun sequence.
ACCESSION   DACADA010000041 DACADA010000000
VERSION     DACADA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.041"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            141..608
                     /gene="slyB"
                     /locus_tag="ISB17_001874"
     CDS             141..608
                     /gene="slyB"
                     /locus_tag="ISB17_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAP1979811.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            complement(655..1089)
                     /gene="slyA"
                     /locus_tag="ISB17_001875"
     CDS             complement(655..1089)
                     /gene="slyA"
                     /locus_tag="ISB17_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAP1979812.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            1290..1526
                     /locus_tag="ISB17_001876"
     CDS             1290..1526
                     /locus_tag="ISB17_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAP1979813.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1529..2386
                     /locus_tag="ISB17_001877"
     CDS             1529..2386
                     /locus_tag="ISB17_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1979814.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2386..4398
                     /locus_tag="ISB17_001878"
     CDS             2386..4398
                     /locus_tag="ISB17_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP1979815.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4399..4920)
                     /gene="sodC"
                     /locus_tag="ISB17_001879"
     CDS             complement(4399..4920)
                     /gene="sodC"
                     /locus_tag="ISB17_001879"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAP1979816.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5001..5897)
                     /locus_tag="ISB17_001880"
     CDS             complement(5001..5897)
                     /locus_tag="ISB17_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP1979817.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5946..6185)
                     /locus_tag="ISB17_001881"
     CDS             complement(5946..6185)
                     /locus_tag="ISB17_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAP1979818.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6288..6887
                     /locus_tag="ISB17_001882"
     CDS             6288..6887
                     /locus_tag="ISB17_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1979819.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6924..8021
                     /gene="nemA"
                     /locus_tag="ISB17_001883"
     CDS             6924..8021
                     /gene="nemA"
                     /locus_tag="ISB17_001883"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAP1979820.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8102..8509
                     /gene="gloA"
                     /locus_tag="ISB17_001884"
     CDS             8102..8509
                     /gene="gloA"
                     /locus_tag="ISB17_001884"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP1979821.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8612..9259
                     /gene="rnt"
                     /locus_tag="ISB17_001885"
     CDS             8612..9259
                     /gene="rnt"
                     /locus_tag="ISB17_001885"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAP1979822.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(9631..9978)
                     /gene="grxD"
                     /locus_tag="ISB17_001886"
     CDS             complement(9631..9978)
                     /gene="grxD"
                     /locus_tag="ISB17_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAP1979823.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            10313..11140
                     /locus_tag="ISB17_001887"
     CDS             10313..11140
                     /locus_tag="ISB17_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP1979824.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            11268..11849
                     /gene="sodB"
                     /locus_tag="ISB17_001888"
     CDS             11268..11849
                     /gene="sodB"
                     /locus_tag="ISB17_001888"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAP1979825.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(11995..13164)
                     /locus_tag="ISB17_001889"
     CDS             complement(11995..13164)
                     /locus_tag="ISB17_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979826.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(13330..13419)
                     /gene="ynhF"
                     /locus_tag="ISB17_001890"
     CDS             complement(13330..13419)
                     /gene="ynhF"
                     /locus_tag="ISB17_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAP1979827.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            13718..14743
                     /gene="purR"
                     /locus_tag="ISB17_001891"
     CDS             13718..14743
                     /gene="purR"
                     /locus_tag="ISB17_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAP1979828.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(14740..15672)
                     /locus_tag="ISB17_001892"
     CDS             complement(14740..15672)
                     /locus_tag="ISB17_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1979829.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            15785..16996
                     /locus_tag="ISB17_001893"
     CDS             15785..16996
                     /locus_tag="ISB17_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAP1979830.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17287..18435
                     /gene="cfa"
                     /locus_tag="ISB17_001894"
     CDS             17287..18435
                     /gene="cfa"
                     /locus_tag="ISB17_001894"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAP1979831.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(18475..19116)
                     /gene="ribE"
                     /locus_tag="ISB17_001895"
     CDS             complement(18475..19116)
                     /gene="ribE"
                     /locus_tag="ISB17_001895"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAP1979832.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            19331..20704
                     /gene="mdtK"
                     /locus_tag="ISB17_001896"
     CDS             19331..20704
                     /gene="mdtK"
                     /locus_tag="ISB17_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAP1979833.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(20745..22001)
                     /locus_tag="ISB17_001897"
     CDS             complement(20745..22001)
                     /locus_tag="ISB17_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979834.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            22309..22385
                     /locus_tag="ISB17_001898"
     tRNA            22309..22385
                     /locus_tag="ISB17_001898"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22343..22345,aa:Val,seq:gac)
     gene            22390..22466
                     /locus_tag="ISB17_001899"
     tRNA            22390..22466
                     /locus_tag="ISB17_001899"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22424..22426,aa:Val,seq:gac)
     gene            22575..22880
                     /locus_tag="ISB17_001900"
     CDS             22575..22880
                     /locus_tag="ISB17_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAP1979835.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            23006..24610
                     /locus_tag="ISB17_001901"
     CDS             23006..24610
                     /locus_tag="ISB17_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAP1979836.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(24622..25434)
                     /locus_tag="ISB17_001902"
     CDS             complement(24622..25434)
                     /locus_tag="ISB17_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979837.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(25438..26223)
                     /locus_tag="ISB17_001903"
     CDS             complement(25438..26223)
                     /locus_tag="ISB17_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAP1979838.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(26220..26888)
                     /locus_tag="ISB17_001904"
     CDS             complement(26220..26888)
                     /locus_tag="ISB17_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1979839.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(26952..27590)
                     /locus_tag="ISB17_001905"
     CDS             complement(26952..27590)
                     /locus_tag="ISB17_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAP1979840.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(27603..29705)
                     /locus_tag="ISB17_001906"
     CDS             complement(27603..29705)
                     /locus_tag="ISB17_001906"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAP1979841.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(29726..30352)
                     /locus_tag="ISB17_001907"
     CDS             complement(29726..30352)
                     /locus_tag="ISB17_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAP1979842.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         7459 a         7921 c         7300 g         7806 t
ORIGIN      
        1 tgtttataat tggttgatcc tactttcatt atgatttgct catatttggt agaacatgta
       61 accatggatt cacatatgcc atatactttg accatgaggg atgcttgcgt ggcgtttcat
      121 ggtgaacagg agatttttca atgattaaac gcgtattggt tgtttcaatg gtaggtctgt
      181 ctcttgtcgg ttgtgttaat aacgacaccc tgtcagggga tgtttatacc gcttctgaag
      241 cgaaacaagt acagaatgtc agctatggca ccatcgttaa tgtacgtcag gtacagattc
      301 agggcggtga tgattccaac gttatcggcg caattggcgg tgctgtactt ggtggtttcc
      361 tgggaaatac tattggtggc ggaaccgggc gttctctggc tactgcagca ggggccgttg
      421 caggtggtgt agccggtcag ggcgtacaga gtgcaatgaa caaaacgcag ggtgtcgagc
      481 tggaaattcg taaagatgat ggcaacacca tcatggtggt acagaaacaa gggaacactc
      541 atttctctcc aggccaacgt gtcgtactgg ccagcaatgg cagtcaggtg accgtttccc
      601 cgcgctaaat aagtttgcgt gtggtcaggt tactgaccac acgccccctt catttcaccc
      661 tttggcctgt aactcaatga tattatgctc aagttttgcg atgagcttaa tcagttgttc
      721 cagttcctct gcggagatgc catgtaaaat ttccgcgcgg gttttgttaa taacagcctc
      781 catttcgctg atcagtggct ctgccttttc cgtcagttta atacgtttag cccgacgatc
      841 gctggcacaa gtttgacgcg aaattaaccc tttttcttcc agttggtcca gcgtacggac
      901 cagtgatggc tgctcgatgc caatcgcttt tgccagttga atttgcgact ggtctggagg
      961 taactgatgg atattgtgta acgtaaccca atgggtttgt gttaactcca gcggtttcag
     1021 gcgatggtct atcagagcac gccatatgcg caccaaccgt gccagatcag aacctagtgg
     1081 cgattccaat ttcatctcct tataattagc ttgctaagat attatgcggc ttttagaata
     1141 gtgtgcagca attgtattgc taaaacaaat gtattgctgc atttggttac cgtcagacat
     1201 atttttcaga aattgcgcgt aaatttttcg cacttaaaga atatttatta atctaacgca
     1261 atatattctg tcgtaaagga atctactttg tgaagtttat gctcaatgca acaggattgc
     1321 ccttacaaga tctggtgttc ggtgcgtccg tctactttcc tccgtttttc aaagccttcg
     1381 cgtttggatt cgtcatctgg cttgtcatac accgcctgct tcgtggctgg atctacgctg
     1441 gtgacatctg gcatcccttg ttaatggatt tatcgctgtt tgcgatttgc gtttgccttg
     1501 ctctggcaat actgattgcg tggtaactat gtcaattaaa acaattaagt atttctcaac
     1561 aatcattgta gcggtagttg cggttcttgc cggatggtgg ctgtggaatt attacatgca
     1621 atctccgtgg acacgcgacg ggaaaatacg cgcagaacag gtttctatca ccccacaggt
     1681 gtcgggacgt attgttgagc tgaatataaa agacaaccag ctggttaacg cgggggatct
     1741 tctgctcacg atcgataaaa cgccctttca gatcgccgag ctgaacgcac aggctcagct
     1801 ggcgaaagcg caatctgacc tcgcaaaagc caataacgaa gcgaatcgtc gtcgtcatct
     1861 ctcacaaaat tttatttctg ccgaagaact cgataccgct aacctcaatg ttaaagcgat
     1921 gcaggccagc gttgatgccg cacaggcgac gctgaaacag gcacaatggc aactggcgca
     1981 aacggaaatt cgcgccccgg tgagtggatg ggtgactaat ctcactaccc gcatcggcga
     2041 ctacgctgac accgggaaac cgctgtttgc ccttgttgac agccactcgt tttacgtcat
     2101 tggttatttt gaagaaacca agttgcgcca tatccgcgaa ggtgcaccag cacaaattac
     2161 cctctatagt gataacaaaa cgttacaggg tcacgtgtcc agtatcggtc gggcgattta
     2221 tgatcagagc gttgaaagtg actcaagcct tattccggat gtgaaaccta acgtcccctg
     2281 ggttcgcctc gcccaacgcg ttcccgtccg ctttacgctg gataaagtcc caggcgatgt
     2341 cacgctggtg tcgggcacca cctgtagcat tgccgtaggt caataatgaa cgcatcgtca
     2401 tggtccttgc gcaatttgcc ctggttcagg gccacgctgg cgcaatggcg ttatgcgtta
     2461 cgcaatacca ttgccatgtg tctggcgctg acggttgcct attatttgaa tctggatgaa
     2521 ccctattggg cgatgacctc ggctgcggtg gttagctttc ccaccgttgg cggtgtcatc
     2581 agcaaaagcc tcggacgcat cgctggcagt ttgctcggag ccattgcagc actgcttctt
     2641 gccggacata cgctcaatga gccgtggttt tttctattga gcatgtcggc gtggcttggc
     2701 ttttgtacct gggcctgtgc gcacttcacg aataacgtcg cgtatgcatt tcaactggcg
     2761 ggctacacgg ctgccatcat cgcctttccg atggttaata ttactgaggc cagccagctg
     2821 tgggatatcg ctcaggcgcg tgtttgcgag gtgatagtcg gtattttgtg cggcggcatg
     2881 atgatgatga tcctgccgag cagttccgat gctacagccc ttttaaccgc attgaaaaac
     2941 atgcacgccc gattactgga acatgccagt ttactctggc ggcctgaaac aaccgatgcc
     3001 attcgtgcag cacatgaagg ggtgattggg cagatactga ccatgaattt gctgcgtatc
     3061 caggctttct ggagccacta tcgttttcgc cagcaaaacg cacgccttaa tgcgctgctc
     3121 caccagcaat tacgtatgac cagtgtcatc tccagcctgc gacgtatgtt gctcaactgg
     3181 ccctcaccgc cagatgccac gcgagaaatt ctcgaacagc tgctgacggc gctcgccagt
     3241 tcgcaaacag atgtttacac cgtcgcacgt attatctccc cactacgccc gaccaacgtc
     3301 accgactacc ggcacatcgc cttctggcag cgactacgtt atttttgccg cctttatctg
     3361 caaagtagtc aggaattaca tcgtctacaa agcggtgtag atgatcatgc cagactccca
     3421 cggacatccg gcctggctcg tcataccgat aacgccgaag ctatgtggag cgggctgcgt
     3481 acattttgta cgttgatgat gattggcgca tggagtattg cttcgcaatg ggatgccggt
     3541 gccaatgctt taacgctggc agcaattagc tgcgtactct actccgccgt cgcagcaccg
     3601 tttaagtctt tgtcactgct gatgcgcacg ctggtgttac tttcgctatt cagctttgtg
     3661 gtcaaatttg gcctgatggt gcagattagc gatctgtggc aatttttact gtttctcttt
     3721 ccactgctgg cgacaatgca gcttcttaaa ttgcagatgc ccaaatttgc cgcactgtgg
     3781 gggcaactga ttgtttttat gggttcgttt atcgctgtca ctaatccccc ggtgtatgat
     3841 tttgctgatt ttcttaacga taatctggca aaaattgttg gcgtcgcgtt ggcgtggtta
     3901 gcgttcgcca ttctgcgtcc aggatcggat gctcgtaaaa gccgccgcca tattcgcgcg
     3961 ctgcgccggg attttgtcga tcaactaagc cgccatccaa cactgagtga aagcgaattt
     4021 gaatcgctca cttatcatca cgtcagtcag ttgagtaaca gccaggatgc gctggctcgc
     4081 cgttggttat tacgctgggg tgtagtgttg ctgaactgtt ctcatgttgt ctggcaattg
     4141 cgcgactggg aatcgcgttc cgatccgtta tcgcgagtac gggataactg tatttcactg
     4201 ttgcggggag tgatgagtga gcgtggcgtt cagcaaaaat cactggcggc cacacttgaa
     4261 gaattacagc ggatttgcga cagccttgcc cgtcatcatc aacctgccgc ccgagaactg
     4321 gcggcaattg tctggcggct gtactgctcg ctttcgcaac ttgagcaagc accgccgcaa
     4381 ggtacgctgg cctcttaatt acttaattac accacaggca tagcgttcac cgccaccgcc
     4441 cagcggtttg ggttgatcgg acatattatc gccgccaacg tggactatca gcgctttgtc
     4501 tttgacttca tccagtgatt tcagacgagg cgcgatgacg gcatcggtcg ccttgccgtc
     4561 attattgact accagtgcag gcagatcgcc taaatgcccg gcaccttctg gcccttcatg
     4621 tttaccggta ttttgtggat caagatgccc gcctgcggat tctgcggcgc tggctttgcc
     4681 atctttggtg gctggctggc agcttccttt ggcatgaata tggaagccat gttcaccggg
     4741 aggtaatgct ttcagatcgg gcgaaaactc cagaccttta tcggtttcag taatggtgac
     4801 gctaccaatt gactgcccaa ccccttgcga cgtgacgagg ttcatctcga ctttttcact
     4861 ggcagcttgt gcgccggtcg caacaaccag cgccagaatg gccagactaa aacgtttcat
     4921 aggacctccg ttcattgtgc attcctgaat aagtgtaaac cagtgacaca tttttgaacg
     4981 gcggctattc ctaaaagtgc ttacggtacg tcgtaaccta gtgccgcttt acggatacgg
     5041 aaccattgtt gacgggtcat tttcagtgtt tctgcttcga ctgctgcccg tacgcgctca
     5101 attttacctg agccgataat tggcagcggc tgcgacggta aacgtaatac ccaggcgtaa
     5161 accacctgtt caatcgagcc cgcgtttaac tcctctgcca ccacagccag ttcatcacgc
     5221 agcggctgga aataatcatc attaaacaga cgaccaccac caaggcagga ccacgccatc
     5281 ggacgaacac gcagttgttg cagttggtcg agcgtgccat ccagcagtaa cggctgatgc
     5341 accggggata tttccacctg attagtggca agggtaaatg gcaggcgtga ttgcaacagc
     5401 gcaaattgcg caggtgtaaa gttcgataca ccaaaatgac gcactttgcc gctctgatgc
     5461 agatgtttaa aggcgtccgc cacttcatcg gcatccatta acgggtctgg tcggtggatt
     5521 aacagcagat ccagatgatc ggtcgcgaga ttaattagcg actgttcggc gctcttaatg
     5581 atgtgatcgc ggtcagtgat gtaatggcca atgacgtttt cttcacacgc ggtcgtcgcg
     5641 ataccgcatt tactgacgat ttccatccgt tcacgcaggt gaggtgccag tttcagcgcc
     5701 tcgccaaacg ccgcttcgca ctgatagcca ccataaatat cagcatggtc cacggtggtc
     5761 acgccgagat ccagatgctc ttcaataaaa ctgaccaact ggcgggcgga catgttccag
     5821 tccatcaatc gccagtagcc catcacaaaa tgggaaaact ccgggccttg cggcgcaata
     5881 gtaatacgct gaaccataat cgcttcctct tatcagatat gagaggagta tacgcaagat
     5941 taggctcaaa agagtgatgg ttgctccggt tcgtctgatg acgctggctt atttgcacgt
     6001 aatttgcgca ttaatcgctg ccgacaaagg cgcaacactt cttgtttttc gccatcgctc
     6061 attttattcc agttaaaacg ctcatcccgg ctacgaaaac agccgcgaca aaatccccgt
     6121 tcgtcagact ggcaaattcc ccggcacggg ctctggacgg gaaagaactc taattgctcc
     6181 gtcacctcgc cctcctcaga taagattatt accattattg aagctgttaa tgtccaaagt
     6241 agcaactttg cttgcactag accgactggt ctactacact ccaacgcatg aacaaacaca
     6301 ccgaacatga tactcgcgaa catctcctgg cgacgggcga gcaactttgc ctgcaacgtg
     6361 gattcaccgg gatggggcta agcgaactcc taaaaaccgc tgaagtgccg aaagggtcct
     6421 tctatcacta ctttcgttct aaagaagcgt ttggcgttgc catgcttgag cgccattacg
     6481 ccgcatatca ccagcgactg actgagttgc tgcaatccgg cgaaggtaac taccgcgacc
     6541 gcatactggc ttattaccag caaacactga accagttttg ccaacatgga accatcagtg
     6601 gttgcctgac agtaaaactc tctgccgaag tgtgcgatct gtcagaagat atgcgcagcg
     6661 caatggataa aggggctcgc ggcgtgatcg ccctgctctc tcaggcgctg gaaaatggcc
     6721 gtgagaacca ttgtttaacc ttttgtggcg aaccgctgca acaggcacaa gtgctttacg
     6781 cactgtggct tggtgcgaat ttgcaggcca aaatttcgcg cagtttcgag ccactggaaa
     6841 acgcgctggc ccatgtaaaa aacattattg cgacgcctgc cgtttagcag gcatttttta
     6901 tcaccagacg accgggagcc tttatgtcat ctgaaaaact gtattcccca ctgaaagtgg
     6961 gcgcgatcac ggcggcaaac cgtattttta tggcaccgct gacgcgtctg cgcagtattg
     7021 aaccgggtga catccctacc ccgttgatgg cggaatacta tcgccaacgt gccagtgccg
     7081 gtttgattat tagcgaagcc acgcaaattt ctgcccaggc aaaaggatac gcaggtgcac
     7141 ctggcatcca tagcccggaa caaattgccg catggaaaaa aattaccgct ggcgttcatg
     7201 ctgaaaatgg tcatatggcc gtacagctgt ggcacaccgg acgcatttct cacgccagcc
     7261 tgcaacctgg cggtcaggca ccggtagcgc cttcagcact tagcgcggga acacgtactt
     7321 ctctgcgcga tgaaaatggt caggcgatcc gtgttgaaac atccatgccg cgtgcgcttg
     7381 aactggaaga gattccgggt atcgtcaatg atttccgtca ggccattgct aacgcgcgtg
     7441 aagccggttt tgatctggta gagctccact ctgctcacgg ttatttgctg catcagttcc
     7501 tttccccttc ttcaaaccat cgtaccgatc agtacggcgg cagcgtggaa aatcgcgcgc
     7561 gtctggtgct ggaagtggtc gatgccggga ttgaagaatg gggtgccgat cgcattggca
     7621 ttcgcatttc gccaatcggt actttccaga acacggataa cggcccgaat gaagaagccg
     7681 atgcactgta tctgattgaa caactgggta aacgcggcat tgcttatctt catatgtcag
     7741 aaccagattg ggcgggaggt gaaccatata ctgatgcgtt tcgcgaaaaa gtacgcgccc
     7801 gtttccacgg tccgattatc ggcgcaggtg catacacagt agaaaaagct gaaacgctga
     7861 tcggcaaagg gttaattgat gcggtagcat ttggtcgtga ctggattgcg aacccggatc
     7921 tggtcgcccg cttgcagcgc aaagctgagc ttaacccgca gcgtgccgaa agtttttatg
     7981 gtggcggtgc tgaaggttac actgattacc cgacgctgta atccaacatt gcgagcggcg
     8041 caaagccgcc gctatactaa aacaactttt tgaatctgtt agccattttg aggatataaa
     8101 gatgcgtctt cttcatacca tgctgcgcgt tggcgatctg caacgctcca tcgattttta
     8161 taccaacgtg ctgggcatga aactgctgcg taccagcgaa aacccggaat acaaatactc
     8221 actggcgttt gttggttacg gcccggaaac cgaagaagca gtgattgaac tgacctacaa
     8281 ctggggcgtg gataaatacg aactcggcac tgcttatggt cacatcgcgc ttagcgtaga
     8341 taacgccgct gaagcgtgcg aaaaaatccg tcaaaacggg ggtaacgtaa cccgtgaagc
     8401 gggtccggta aaaggcggta ctacggttat cgcatttgtg gaagatccgg acggttacaa
     8461 aattgaatta atcgaagaga aagacgccgg tcgcggtctg ggcaactaat ctcctgccgg
     8521 gcgtgaactc atcgcgcccg catctttact gcatcgacaa gtaatatttg tcataatgcg
     8581 cgctgcaatt tatccgtatt aagagaatca gatgtccgat aacgctcaac ttaccggtct
     8641 gtgcgaccgt tttcgtggtt tttatcctgt tgtgatcgat gttgaaacag ccggatttaa
     8701 cgccaaaacc gatgcgctgc ttgagattgc cgccatcacc ctgaaaatgg atgaacaagg
     8761 ctggctgatg ccggacacca cattacattt ccacgtcgaa ccatttgtcg gcgcaaattt
     8821 gcaaccagag gcccttgcct tcaatggcat tgatccgaac gatcccgatc gcggcgcggt
     8881 cagcgagtac gaggcgctgc acgaaatttt taaagttgta cgtaaaggta ttaaagcgag
     8941 cggctgtaac cgcgccatta tggtggcgca caacgccaat ttcgatcaca gctttatgat
     9001 ggccgccgca gaacgcgcct cactgaaacg taacccgttc caccctttcg ccacttttga
     9061 cactgctgca ctggccgggc tggcactcgg acaaaccgta ttgtcaaagg cttgccagac
     9121 agctggcatg gacttcgaca gcacccaggc gcactccgcg ctgtacgaca ccgaacgcac
     9181 tgctgtgctg ttttgtgaaa tcgtcaaccg ctggaaacgt ctgggaggct ggccactacc
     9241 tgccgccgca gaggtgtaat cgagtcgatg cctgatgaca tgcaatgatt caggcatcta
     9301 tagtgaggct attccacgca tcatgcatga tattcacggg gaatagcgtt aatggcaaat
     9361 aatccaaacc cttcatcgcc cctgccggac gtgttttcgc cggcgacccg cgactggttt
     9421 cttcgcgcct ttaaacagcc gaccgctgtc tagtcgcgaa ccacggctgc gctttacgct
     9481 aacagaagtg aatgacctac cggtccggca aacgccgatg tttacgctgt tacgtgaggc
     9541 gggattttca agttcgccac aagggctgga ttggggatag agaaaggact gacggatgcc
     9601 cgttcgcatc cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta
     9661 tttagcggca gtttctttga tcagctgctg cagttcacca cgctgataca tttcgatcac
     9721 gatatcacaa ccgcccacca gctcgccgtc aacccacagt tgcgggaagg tcggccagtt
     9781 cgcatatttc ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa
     9841 acgttcgcca catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag
     9901 tttcggtgaa cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt
     9961 ttcgatagtg gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat
    10021 tgtagcgggt cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc
    10081 cctttgctct atttgttgca tttcaaatat tcgtttcttc cctgatgcac ggcaaactgg
    10141 cgtatttata accattttta gctttcactg ctgcattttt ttgcgctcgc cgacgaaacg
    10201 tattttttaa caataaaagc tattaacttt ctcttcttct atgcattaga atcatcaagt
    10261 tttgtaaatc agacgcaggc atgatagacc tgccttaaca gagggacgct cagtggcgcg
    10321 gataaaccgt atttcgatca cgctctgtgc tttacttttt accaccctgc ctttaacgcc
    10381 tatggcccat gcttcaaagc aagccaggga gagttctgct accactcata tcaccaaaaa
    10441 agcagataaa aagaaaagca cggcaaccac ccaaaagacc cagaaagcca gccagaaaac
    10501 agcgaaaaaa gccgccagta aaagtacgac caaaagcaaa accgcttctt ccgttaaaaa
    10561 atcttccatt accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac
    10621 ggcctcagcc agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt
    10681 gaaagtcaaa aatgccgcgt caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa
    10741 agccacaaaa gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg
    10801 tggcagctca ccgcgtaccg gttttgattg cagcggtctg gtttattacg cttataaaga
    10861 tttggtgaaa attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gcgatgcagc
    10921 gccaatcgaa cgtagtgaac tgaaaaacgg cgacctggtc ttcttccgta ctcagggacg
    10981 cggtacagcc gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg
    11041 cacgggccag gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt
    11101 tggcgctcgc cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg
    11161 gcaacagggt aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac
    11221 aataaggcta ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat
    11281 tacctgcact accatatgct aaagatgctc tggcaccgca catttctgcg gaaaccatcg
    11341 agtatcacta cggcaaacat catcagactt atgtcactaa cctgaacaac ctgatcaaag
    11401 gtaccgcgtt tgaaggtaaa ccactggaag agattattcg cagctctgaa ggtggcgtat
    11461 tcaacaacgc agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg
    11521 cgggtggcga accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg
    11581 ccgatttcaa agctcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct
    11641 ggctggtgaa aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc
    11701 cactgaccac cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca
    11761 tcgactatcg caatgcacgc cctggctatc tggagcactt ctgggcgctg gtgaactggg
    11821 aattcgtagc gaaaaatctc gctgcataat aactgatggc aaatgcaaca ttacctgatg
    11881 cgcttcgctt accatgccaa cgcggatgag gcgttaacag cgcattcggc aagataaatc
    11941 gcacgttgcc agcaactgta acgcagaagg ttatccttct gcgtttttgt ttaattagct
    12001 gttggcaacg caaactgctt caggttgttt tctggctgac ataaacacca gcaataatgc
    12061 cagtcccgcg acaatcgccc ccatcaccgg cacaaaactg tatcccagcc cagcggaaat
    12121 taccgcacca ccagcagctg ctcccagcgc atttccaaga ttaaaggcac caatattgac
    12181 tgatgaagac agacctggcg cttcactggc gacacgcatc acgcgcatct gtaacggcgg
    12241 tacgatcgca aaggttgcag ctccccacac caccatgcta atagctgcgc cgaactcatt
    12301 gcgggccagg aacgggattg ccagcataat caccatcagc aacaacaaaa agcctttcaa
    12361 cgtgccgtta actgaacgat ctgccagttt gccgccgaga tagttaccga tagagaatcc
    12421 gacaccaatc agcaccagca ttgccgtgac gaacaccggc gttacgtggg taatgctttg
    12481 cagtaccgga gagatatagg tgtagagggt aaacattgca ccagcgccca gtaccgtcgt
    12541 cagcaatgca gacagcacct gcggacgcat taataccgcc agctcttttt tcacttcagg
    12601 tcgcgcccct gcgccacctt taggtaatga gaggaacaga cttaccattg aaatcacgcc
    12661 cagccccgct gttgccagaa atgacatccg ccagccgatg gtttcgccca accaggtcgc
    12721 cgccggcacg ccaccgatat ttgccagggt taaccccata aacatagtgg caactgcgct
    12781 ggcctgttta tgttttggca ctacgcttgc ggccacgact gaacccaaac caaaaaatgc
    12841 cccgtgattc aggctggtca aaatgcgtga aagcatcagg gtcatataat ccggcgcgat
    12901 ggcggaaagt acgttgccaa gcgtgaaaat cgccatcagg aaaatcaacg cactgcggcg
    12961 ggcacgatga gaaagtagaa gcgtcatcag cggcgcgcca accattacgc caactgcata
    13021 agcactgatt aacattccgg cagcgggaat cgagacatcc acaccgcgcg caatgacggg
    13081 caacaagccc attggcgaga actccgttgt cccgatacca aacgcgccaa tcgccagcgc
    13141 cagcaacgga tagttaattt tcatgcctta tctccacctc ttcgcgtcat tacgcgatat
    13201 tcattaaagt ggcgaaagca tgacagtaat cacaaaaaaa tgaaaataac aaaaagagaa
    13261 aacacttttg ccattttgct aacaaacagg aaggagatgc gagggagaac gcgctccctc
    13321 gagaggaaat cagtgcagcg cggcagtcaa acccacggcc acgatcaaac cgaggacgat
    13381 aatcgttgtt accagtgaaa atttaaggtc ggtgctcatc aagttttctc cttttttatt
    13441 accacacaaa aagtgatatt acgcattttt acacactgtg atgaaaaaat ctcccgtcat
    13501 ttataatgat aagtgttttt actacttccc cttttcgtca agatcggcca aaattccacg
    13561 cttacactat ttgcgtactg gccattgacc ccttcctgac gctccgtgtc gtttttccgg
    13621 cgtaccgcaa catttttgtt gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgaat
    13681 ttgcaggagc tgaagttagg gtctggagtg aaatggaatg gcaacaataa aagatgtagc
    13741 gaaacgagca aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt
    13801 cgctgaagaa acgcgcaatg ccgtgtgggc agcaatcaaa gaattacact actcccctag
    13861 cgcggtggcg cgtagcctga aggttaacca caccaagtct atcggtttgc tggcgaccag
    13921 cagcgaagcg gcctattttg ccgagatcat tgaagcggtc gaaaaaaatt gcttccagaa
    13981 aggttacacc ctgattctgg gcaatgcgtg gaacaatctt gagaaacagc gggcttatct
    14041 gtcgatgatg gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg agtacccaga
    14101 gccgttgctg gcgatgctgg aagagtatcg ccatatccca atggtggtga tggactgggg
    14161 tgaagcaaaa gctgacttca ccgatgcggt cattgataac gcgttcgaag gcggctacat
    14221 ggccgggcgt tatctgattg aacgcggtca tcgcgaaatc ggcgttatcc ctggcccgct
    14281 ggaacgtaac accggcgcag gccgtcttgc tggttttatg aaggcgatgg aagaagcgat
    14341 gatcaaagtg ccggaaagct ggattgtgca gggtgacttt gaacctgaat ccggttatcg
    14401 cgccatgcag caaatcctgt cgcagtcgca tcgccctact gccgtcttct gtggtggcga
    14461 tatcatggca atgggcgcac tttgtgctgc tgatgaaatg ggtctgcgcg tgccgcagga
    14521 tgtttcgctg atcggttatg ataacgtgcg caacgcccgc tattttacgc cggcgctgac
    14581 cacgatccat cagccaaaag attcgctggg cgaaacagcg ttcaacatgc tgttggatcg
    14641 tatcgtcaac aaacgtgaag aaccgcagtc cattgaagtg catccgcggt tgattgaacg
    14701 ccgctccgtg gctgacggcc cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt
    14761 ctcttccggc tcccgcagcc actccttatt cagcgtctca ctatcgccga gatactcaag
    14821 caaccaggtt aacgcaggcg acatatcatt ttgctgccat gtcagacaac atgccgaatc
    14881 cgggaagggg ttttccagtt ttagtgctac ccacttcccc tcattaagcc acggtttggc
    14941 gaaatgtgtt ggcaccatcc ctatgcataa tcctgccgag atacaggttg ccgatgattc
    15001 ccagtcaggc acgacaagtc ttttttggtt atccagcaac caggtaatac gtttaggtag
    15061 cgttcgcgag gtgtcttcgc gcaccaatga cggccagttg cgcaacgtat catcgctgaa
    15121 cgggccatcc atcaacgcca gcgggtggtg gctggcaaca acgcaactcc agcttaacat
    15181 ccccatatcc cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg cgccaatcgc
    15241 cagttccacg cgcccgtcgg aaagtgcatc ccagacaccg ttgaacactt cctgaaagac
    15301 aaggagttcg acatcatcaa aatggcgata aaaatcaacg atcatctgcc gtgttctctc
    15361 tggcctgaca atattatcca ctgcgatagc taactgaccg cgccagccgt tcgctatctg
    15421 ctgacattgc tggcgggtga tctgcatttt tttgacaaca gagcgccctt ctttgagaaa
    15481 ccacgccccg gctgcggtca gttccacatc acggtgacgt cgttcaaaca gcggcaccgc
    15541 cagccactct tccagctgac gcacggtata gctgaccgca gaaggaacgc gatgcaactc
    15601 ctgggctgcg gcgctaaaac taccattacg cgctaccgca tcaacaactt cgagagaata
    15661 ttctgaccac atagtctgcc tgcaaaattt ttgaaaccaa tcatcaaata ttaccgtttc
    15721 acaacactaa tttcactccc tacactttgc ggcggtgttt aattgagaga tttagagaat
    15781 atacatgcaa cctgggaaaa gatttttagt ctggctggcg ggtttgagcg tactcggttt
    15841 tctggcaacc gatatgtatc tgcctgcttt cgccgccata caggccgacc tgcaaacgcc
    15901 tgcgtctgct gtcagtgcca gccttagtct gttccttgcc ggttttgccg cagcccagct
    15961 tctgtggggg ccactctccg accgttatgg tcgcaaaccg gtgttattca tcggcctgac
    16021 aatttttgcg ttaggtagtc tggggatgct gtgggtggaa aacgccgcca cgctgctgat
    16081 attgcgtttt gtacaggctg tgggtgtctg cgccgcggcg gttatctggc aagcattagt
    16141 gacagattat tatccttcac agaaagttaa ccgtattttt gcgaccatca tgccgctggt
    16201 gggtctatct ccggcactgg ctcctctgtt aggaagctgg ctgctggtcc atttttcctg
    16261 gcaggcgatt ttcgccaccc tgtttgccat taccgtggtg ctgattctgc ctattttctg
    16321 gctcaaaccc acgacgaagg cccgtaacaa tagtcaggat ggtctgacct ttaccgacct
    16381 gctacgttct aaaacctatc gcggcaacgt gctgatatac gcagcctgtt cagccagttt
    16441 ttttgcatgg ctgaccggtt caccgttcat ccttagtgaa atgggttaca gcccggcagt
    16501 tattggttta agttatgtcc cgcaaactat cgcgtttctg attggtggtt atggctgtcg
    16561 cgccgcgctg cagaaatggc aaggcaagca gttattaccg tggttgctgg tactgtttgc
    16621 tgtcagcgtc attgcgacct gggctgcggg cttcattagc catgtgtcgt tggtcgaaat
    16681 cctgatccca ttctgtgtga tggcgattgc caacggcgcg atctacccga ttgttgttgc
    16741 ccaggcgctg cgtcccttcc cacatgcaac cggtcgcgcc gcagcgttgc agaacactct
    16801 gcaactgggt ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatca gtatcagtac
    16861 gccattgctc accaccacca gcgtgatgtt atcaacagta gtgctggtcg ctctgggtta
    16921 catgatgcaa cgttgtgaag aagttggctg ccagaatcat ggcaatgccg aagtcgctca
    16981 tagtgaatca cactgatcta tatcgatata cttatactta ggctgctaac aaaattttgt
    17041 tgtatgattg aaattagcgg cctatactaa tttcgagttg ttaaaactac gataaatatt
    17101 atgttattac ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt
    17161 tcctcctttc cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc
    17221 tcacaaagcc caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac cagtgatgga
    17281 gaaactatga gttcatcgtg tatagaagaa gtcagtgtac cagatgacaa ctggtaccgt
    17341 atcgccaacg aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat
    17401 attcgtgtca aaaaccccga tttttttaaa cgcgttctgc aggaaggatc gttggggtta
    17461 ggcgaaagtt atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa
    17521 gtcttacgcg caggtctcga gaaccaactt ccccatcatt tcaaagacac gctgcgtatt
    17581 gccggcgctc gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaagag
    17641 cattacgatt tgggtaatga cttgttcagc cgcatgcttg accccttcat gcaatattcc
    17701 tgtgcttact ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg
    17761 atttgtgaaa aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgtggctgg
    17821 ggcggactgg cgcactacat ggcgtctaat tatgacgtaa gcgtggtcgg cgtcaccatt
    17881 tctgccgaac agcaaaaaat ggctcaggaa cgctgtgaag gcctggatgt caccattttg
    17941 ctgcaagatt atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc
    18001 gaacacgtcg gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa
    18061 ccggaaggta tattcctgct ccataccatc ggttcgaaaa aaaccgatct gaatgttgat
    18121 ccctggatta ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagatagct
    18181 cagtccagcg aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat
    18241 actacgttga tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac
    18301 tacagtgaac gctttaaacg gatgtttacc tattatctga atgcctgtgc aggtgctttc
    18361 cgcgcccgtg atattcagct ctggcaggtc gtgttctcac gcggtgttga aaacggcctt
    18421 cgagtcgctc gctaaaggct attctatcgc ccccgctccg ggggcgattt cagatcaggc
    18481 ttctgtacct ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc
    18541 taccactgcc tgagtttgtg gatcgatttc aatgttgacg cgtgcgccga gttttttctt
    18601 cccaagagtc gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt
    18661 gacttcgccg acggtcaggc taataccgtc gatgccaata aatcctttgt acaggatata
    18721 tttcatcaac tgactatcct ggactttaaa ccagatctga cgattatttt ccgaggttaa
    18781 tattttcgcc acttcagcgg tggtcataat atgacctgac attaagtgtc cgccaatttc
    18841 atcgctgaat ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcaccaag
    18901 attagtaatg cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc
    18961 cgtcacggtc aggcagcaac cgttatgcgc cacggacgca ccggtttcca gtccgtccag
    19021 catgtggtcg ggtaactcca ccacatgcgt gcgaaaattt ggtttctcat caatcgacac
    19081 cagttttgcg gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta
    19141 agacattctg ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa
    19201 tggctatttt ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg
    19261 cttcttcttt ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa
    19321 aggtgttcac gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc
    19381 ggtgattctc gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg tgatggcggg
    19441 cggctatagt gccaccgaca tggcggcggt tgctatcgga acttctatct ggcttccggc
    19501 gatcctcttt ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg
    19561 ttccggtcga cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt
    19621 tgtttccgtt ctcattatgc tggtactgtg gaatgcgggt tacattatcc gctccatgga
    19681 aaacatcgat ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgctatgggg
    19741 cgcgccgggc tatcttttct tccaggttgc ccgtaaccag tgtgaaggtc tggcaaaaac
    19801 caagccgggt atggtgatgg gctttatcgg cctgctggtg aacattccgg tgaactatat
    19861 ctttatttat ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac
    19921 tgcggcggtg tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc
    19981 gatgcgcgat attcgtaacg aaaaaggcac cgcgaagccc gatcctgcgg ttatgaaacg
    20041 actgattcaa ctcggtttgc caattgcgct ggcactgttc tttgaagtaa cgctgtttgc
    20101 cgtcgtggct ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac atcagattgc
    20161 cctgaacttt agttcactga tgttcgtgct tcccatgtcg ctggcggcag cggtaaccat
    20221 ccgcgtaggt tatcgtctgg gtcaaggctc aacgctggat gcgcaaaccg ctgcgcggac
    20281 cgggcttatg gtgggtgtct gtatggcaac cctgacggcc atattcacgg tttcactgcg
    20341 agagcaaatc gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt
    20401 gatgctgctg gcggcggtat atcagatttc tgactctatc caggtgattg gcagtgggat
    20461 tttgcgtggt tataaagata cacgttccat tttctatatc acctttacgg cttactgggt
    20521 gctgggcttg ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg
    20581 gccagcaggc ttctggatag gctttattat tggcctgact tcggcagcca ttatgatgat
    20641 gctgcgtatg cggttcctgc aacgtctgcc gtcagccatc attctgcaac gagcagctcg
    20701 ctagtaaaga caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc
    20761 gttgcgccaa tcccgtcttc gtctggctgt agtttcagtg cgttacacag agtttgccga
    20821 ctggcatctg ttatctcaac gtgtatatca ccagtaactt cccctttcac attgatgaag
    20881 tcaggtgaaa tttctttcgc actggtttgc aggcaaatgg ctccctctcc gcgcaggcgt
    20941 ttcactttca gttgcacatg cgttgccgca tcagtgatca gccacagttt tccgccattt
    21001 agattgatgt taccaatacc aaaagtgaca ctgtcacctt ttaccgtact gccgtcgaga
    21061 atcaatacac cgccctgctg tacagtaatc gttgattgtt gcagatctgc gccatagcca
    21121 ataatcattg aagcgccgtc cagtaatgca acactaccgg tcagttcaac cggtgcgcga
    21181 tcttcctcta cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat cactgtccca
    21241 ccttgtatta ccgcattttc cagcgtaccg gcatgcactt cagcccgacc acccacatta
    21301 acggacaggt tttgcgtctt accggaactg tagagctgaa gaccatccgt ccccagacga
    21361 tagatggctc cattctcaac aatagtgtca gagtcttcac caccgagatt attcatggtg
    21421 ccaccagcaa gcaacgtatc actggctttc cccgccagca taaaaacgcc gccctgatta
    21481 atctcacaat tgcaggcttc cccacctgga tacaccaaca attcaccacc ttcatctacc
    21541 acgaccgctg aggttgtccc attgaccaac aggccaccct cttgatcgag aataattttt
    21601 tccgcgcgat ggccttccag tacacgcagg ttaccgccat tttccagcaa caaaccgcag
    21661 gcataacctt tatcaacgct gaattcacct tcgggatggc ggccattaac cgtagcgagc
    21721 gtagagagac taattgcgcc tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg
    21781 ctgaccgata gtgtgccatt gttaccgtac tgtgtgcatt tggctatccc accggttgcc
    21841 actttcaacc agccagcccc cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca
    21901 atcaactcgc cttcagtaag ttgcgtcgcg ccaataacct cgccggtctt aacaatttgc
    21961 acgttcccgt cgctcatcaa gtttttcgca tcagatccca tgatttattc ctttgctgca
    22021 tctgtgtgcc tttattgcta cctaagtgta aaggctacga aggatttatc cacgacagat
    22081 ttgagatggt ggcaaacaac tctgtttaaa ctctgataca cggattattg ggttgtatca
    22141 gatgtaaatg cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata tagccaattt
    22201 gcttaaaagc tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac
    22261 cttcccactc tgccgctaat attcgtcccc gttgtcacct acaacgttgc gttcatagct
    22321 cagttggtta gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc caattgaacg
    22381 caccatcctg cgtccgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgg
    22441 tggttcgagt ccactcggac gcaccagatt ttcttaatct ggtcttctcc ttttttccct
    22501 ctgtttcttc tctgtatcca atacgttaaa agatttacac tgtcttcgta tgcgttatca
    22561 gaaggagaat cgctatggca actttgttac aacttcattt ttctttcaat ggcccatttg
    22621 gcgacacaat ggctgagcag cttaaaccgc ttgctgagtc gattaatcag gaacctggtt
    22681 ttctgtggaa ggtttggaca gaaagtgaaa agaaccacga agccggtggg atctaccttt
    22741 tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca tactgcccga ctgaaaaatc
    22801 tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgtgccactg agtcaaatca
    22861 atcaggcaaa acttgcctga cagattttaa tcaagggcgg ttagtgccct tttcatccct
    22921 gtctgaaatt tctcaaattc taaaaatctc aaccaaactt acctgataac actaaattcg
    22981 aaagaatgcg tacaggtaag taacaatgaa aacaattgct attgtgggtg ccgggcctac
    23041 ggggatctac accttattct cgcttctaca gcaacaaacg ccactttcta tttctatctt
    23101 cgatcaggct gacgaagccg gtatcgggat gccatacagt gatgaggaaa actcaaaact
    23161 gatgctggca aacattgcca gtattgaaat accgccgatt tattgcacgt atctcgaatg
    23221 gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag aaaccttgca
    23281 cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc aatttttacg
    23341 attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat catgccaggt
    23401 taccgatctg caaattacaa atgctggcgt catgctcgct acaaatctga atttacccag
    23461 cgagacgttt gatttagcgg tgatcgcaac ggggcacgtc tggcctgatg aagaagaagc
    23521 aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg
    23581 taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg cagtggctat
    23641 tcagcatggt tcgttcattg aagatgataa acaacacgtc acttttcacc gcgataacgc
    23701 aaatgaaaag ctaaatatta cgttaatgtc gcgcacaggt attttacccg aagccgattt
    23761 ctattgccct attccctacg agcccttaca catcatcacc gatcaggcat taaatgctga
    23821 gattcaaaaa ggcgaagtgg gccttttgga tcgggtattt agattgatag tagaggaaat
    23881 caagtttgct gatccagact ggagccaacg catagcctta gagagcctga atgtcgattc
    23941 ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact gggcagaaga
    24001 aaatctccag gaagtcgaac gcaataaacg agaaaaacat actgttccct ggcgttatgt
    24061 cattctgcgc ctgcatgaag ccgtacagga aattgttcca catctgaatg aacacgacca
    24121 taaacggttc agtaaaggcc ttgcccgggt tttcatcgat aattatgcgg caatcccttc
    24181 agagtctatt cgtcgactac tggccttacg tgaagcggga atcattcata ttctcgccct
    24241 cggtgaagac tacgaaatgg aaattaatga gtcgcgcacc gtcctgaaaa cggaagacaa
    24301 tagctactcg tttgacgttt ttattgatgc ccgcgggcag cgtccgctta aagtgaaaga
    24361 tattcctttc cctggactac gcgaacaatt acagaaaaca ggggatgaaa tccctaatgt
    24421 tggcgaagat tatacgttac agcaacccga agatattcgt gggcgcgtag cgttcggcgc
    24481 gttgccctgg ttgatgcacg accagccttt cgttcaggga attacggcat gtgcagaaat
    24541 tggagaggcg atggctcggg cggtcgtaaa acctgcatcc cgtgcgcgtc ggcgtctttc
    24601 gtttgattaa aggtaaagct atcagcactc aacaaactga tgttgttcat taagacgata
    24661 gcggactccg gctcgaatat tgttttcacc ttcaatggcg acggcgaagc gaactctttc
    24721 gccgtcatga tatgccagtg cggcgcatcc ccctggcccg agaataattg agtcgaccac
    24781 gcccgtgctc actaccgttg aattttcacc actggcaatg atgtgtacat tgtcgccgct
    24841 gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc accaggtcac cattactggc
    24901 gacatgacac cgttcaccca atgtacaaac gcgtactcgc attccggtat tcgcaatccg
    24961 accgctatct ccagcgctgc ttatccgcga agaattaccg gttacagcaa cccgtgcgcg
    25021 tgcaccggag ctgccaatgt gactgttaaa gccgacactt ccaatccgca cgctataacc
    25081 catactggca atttgcgcgg catacccagc ggttgcgatt ctggcgttat cctcacaaca
    25141 gccgaggcga gcatgatcgt cgctgcggac aaactgactg caaaatagtc gttctccggg
    25201 atctgtggcc agtttttgca tagaggaaac ttcctgatgc gcgaagtttt catctgccag
    25261 ccattttttc cacgcatact caaccagact ctccgcccag tccgtatatc cttcctcaat
    25321 cagtgcatga tgaatatcgg cataactacc gccacgtggg aaatggcgca gaaaccagcg
    25381 atacatcacc gctccgatac gccattctcg caggtctgct cgtgtaatga tcattcgtta
    25441 gtgccggtga tagccatcga ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt
    25501 gcaaagataa agatgaccga agataaaaaa gaggcttata aatgccagag caaaatgcgc
    25561 ctgcaataac cagtatctta cgccaggaaa cacatctccc acggcttgcg gatagagaca
    25621 cagcagcccc gtcaatagta acaatggcaa caatccatac atgacaccaa cataggcgac
    25681 ctgctgtaag ggattaaatt tagactgggt tgttgccggg aaaggatgtt cttccccctg
    25741 cataatgcca aacaaataaa atcgcgtttg ttttgccgct cgatccagcc aaccctgacg
    25801 acgaatgcga tagtggtgac cattaccccc aacggcattg atcagaacaa agccaagcca
    25861 gcatgccagt aacaaaaatc cgcaaacttc atgcaccgca accagacttt taaccgcagt
    25921 tgcgcccacc agcgcaaagt gatttatcag cccacttgcc agcaacaata caaagagtaa
    25981 cgcattcgac cagtgccata atctgaccgc tttgctgtaa agataaatcc tctcactatg
    26041 atcggtggct gatttcttca cgccacgagc acgaagcaag gcatgtagcc ccagcaccaa
    26101 ccacatccca accagcaata gtccggcaat gatcaaccac accggccaaa attggggggt
    26161 gaactgtggc acatagttcg ccaactggct ctgaaactgt tcagcatgtt gcgacgggtt
    26221 catacatttt cctttttaat caaatgttga ccgaaccggc gatacagatg cggtttcccg
    26281 gcgccaggta gctgatattg atagtattta ttttcctgca accacgcctg aatttctggg
    26341 ctatcttcgt gaccaaagat caatgcgtgt tccgggcagg cactgacgca aatgggcgga
    26401 aagccttttg ccagtcgggt ctcagcgcag aaatcacatt tatctgccac tttagtgaca
    26461 gggttaaggt agcgaacctg gtacgggcaa acgccaatgc aataactgca gccgatgcac
    26521 tgcgattttt ctacccgcac gatcccctgc tcatcacgcc acgacgcccc tgtcgggcaa
    26581 acgtcgatgc aaggagcatc ttcgcagtgc tgacatgact gacgaaaaaa gtgatactgc
    26641 gttccattgt cgttatcgat gactggaatg tgtgctatcg ataaacggct tccctgagcg
    26701 gggacgtggt tagttttacg gcatgcgcgg gcgcagatat tacagccatt gcaccgtgac
    26761 tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc tcgtattcgc taacagagaa
    26821 gacgcagaac cagtaaaaaa gattactgtt cccatcccca gaacaaattt gcgtcgagtg
    26881 aaggacatct gattatcccc taatgcagtg gctgcaactg tccaccctat ccatgacatc
    26941 cccctgaaat ttcaggggtt ctgaaactgc tctacctcga accattcgca taagccatac
    27001 gccttgccat aggtcataaa tcgctcgcta aacaagtaca acactgccgt cgatgactcc
    27061 acctgccgta tatcggcata ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc
    27121 tgcgcttcct ggaagtaata tggcgcttgc atcagcatgg cgactttgta aggacgagga
    27181 tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca
    27241 acttgcaaag acatcgccgc gtagttttca ctcatggcct gcgttgagta atagtaatca
    27301 tcctggcttc ccgtcataca agcgatatcc gcgtagtctc cgttctgctt aatgttttcc
    27361 agcaaaactg acagttcctc ttctgcaacc gaatagggtg gcgagagaaa cacggcgcgc
    27421 gccaccagct gcccagccgc agaatgctgg cgaatgaact ctgccaggat ttcagccggt
    27481 tctggttcgg ttaccgtatc aacggggtga cgcatcccct gcaaccattg ccgcttcgcc
    27541 tcatcaattt cgctgacttt cgctaacggc aattcatcct gatggttcat ctctcccatt
    27601 ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg caatatcttc cagccccaga
    27661 cgctgcaatg tttcgcgggt cggacaacca agctgtgggt cccagcccat ttctttgtag
    27721 aacatcgtga gcgaagcatg catatcgtca cggtccattt tgtctgtacc ttcagtaaag
    27781 accgggatct gcggatcctt gtcgaatacc caggaacaga taagatcgtg ttcattacgc
    27841 atatctttgg tttgcatcag ttttaccgtg taggcgcgat gcaacgtaaa gatacgctct
    27901 gcagctaaat ctaatttttc ctgagtcata tcttcgccag tgatcgcttt gaagaatttg
    27961 gcttcaagcg ccagatcacc ccggtaatta cggcttttca gcggcgaaac ggtcattggc
    28021 cagacccagt tgcacagggt gacggcgtta tgcaaacaga cccgcaacag cgaccactta
    28081 gcatatttaa tttttgcgtc attgatcggt gtgtagtttt tggtttcatc gtaagcatct
    28141 tcagaaccaa aaagctcttt cgccacttca cgctgcagtt tcaatggcaa gccggaacca
    28201 ataaaattga tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggaa
    28261 ccgacttgcg ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt
    28321 ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc gatggcatat
    28381 gaaccatcag ccaggtgact caactcaccc acgcgatgcg ccagacggta gtaaaaatct
    28441 ttaataaagt taacgtcacc cgcttccagt tgatcccagc gaatttccgc atactcttca
    28501 gcaggcagaa cacgcttgaa cacacctttg ctgtaacaat aagtaaagtc gcgatgcaac
    28561 tgcccgtagt tacaccatag gccgtagtca tcgaacaaat tcagaccaac caggttacca
    28621 atcacacgac catcgtcctt atcttcaaaa tctttcggcc cgttcgggaa gatggtggta
    28681 tggacaaagt ttgctacaca agtgttacca cctgtgctgg gcacgccaaa ctctttcacc
    28741 cgaggaatat tcatttgggt catacaacgg atcgggcaag agtggcagcc actcattttt
    28801 actgtgtatt tctctgccgc cggacctaag tcaaaaacgg atttataggt acgaaagccg
    28861 accgtattct gattgcctgg cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg
    28921 ccccaaaaca gccctttacg tgctgtccaa cgtgacttgg gatctgaata ctctgcccac
    28981 gattgcggcg tacttggcac gacatggttg ttattcgcac cgataagttc agtcatcatg
    29041 taatcgttga gacgcttcat ctcctgacga tcggcaatgt tgaccccttt cgtcccttca
    29101 accgcaatcg ctttcaggtt tttcgaaccc attattgcgc cagttcccgc accgccgctg
    29161 tggttacggc tattcaacat gccagagaga ggaacaaggt tttctccagc ctgaccaata
    29221 gccgccacac aggtttccgg actggtcaag cgacaaattt cttccgtcgt tgcgcgcgtc
    29281 ccttttcccc ataagaaatc ggctttttca aggctaactt tgtcatcttt aatcttcagc
    29341 cataccggtg atttcgcttt cccttcgata ataatgacgt cgtatccagc gaatttcatt
    29401 tgcgctgcaa aaaagccccc catatgggca tcgacgacta aatttccttt ggtaaaagta
    29461 gaaagtgagg tgatatttac gcgagaacta cagggggcac cagatccagt taatgggccg
    29521 gtagcaaaaa ctaatttatt cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca
    29581 tacataattt tgtagccgaa gcccatgcca ccgacaaaac ttttaaattt actggaatct
    29641 tcgagggtaa tatttcctgt cgtgagattg actcttaata tattacctgt ccaaccgtta
    29701 gccatgattt tttcctttgc aagattcaca cagtaatatc tttccactcg ataattttta
    29761 acgcccctgt tgggcaggcg tttgcgcatt caccgcataa cacacatttc gaggattttt
    29821 tactttcggt atttacggtg gccatcatcc acgggcatgc cgtagtacag gcgctacagc
    29881 caatacaacg tttatgatcg acggtaatac agccttcttt ctgctgccag gtaatcgcac
    29941 caatcgggca gacgttcatg cattgcgact ctttgcattg acggcaggta tccgcggtat
    30001 agttgagatc gccatacagg ccaccgccag agccaacccc gttgtcgcca aagaaataat
    30061 tgcgatggat tttgatacgg gagaagaatg tccctactga gccatcgttg aagttggtac
    30121 aagagatttc acagcggtgg caacccgtgc atcgcgcacg ttgggtcacc agcacccctt
    30181 tgggtgtatt tatcaaccct actgtgccgc tatcaatatc ttcctgtttg caacccaaaa
    30241 gtgacagtaa agcaggggca attgttaaac cagcaaggcc ttttcctgat atacgcaaga
    30301 attctaatcg cgttaagcca atatttaata gtggacgatc aaccgggttc atttatttcg
    30361 catcctcttc gcaaacacag tgctgacaat tcaacagcct gatattaagc actcaactaa
    30421 tacgctgctg acgctgtcct caacaccaga tattttccct aacccgatag tgtatgagaa
    30481 aagatt
//
LOCUS       DACADA010000042        30248 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.042, whole genome shotgun sequence.
ACCESSION   DACADA010000042 DACADA010000000
VERSION     DACADA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.042"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(111..389)
                     /locus_tag="ISB17_001908"
     CDS             complement(111..389)
                     /locus_tag="ISB17_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979843.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(748..1038)
                     /locus_tag="ISB17_001909"
     CDS             complement(748..1038)
                     /locus_tag="ISB17_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1979844.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1039..1350)
                     /locus_tag="ISB17_001910"
     CDS             complement(1039..1350)
                     /locus_tag="ISB17_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1979845.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1579..2487
                     /locus_tag="ISB17_001911"
     CDS             1579..2487
                     /locus_tag="ISB17_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1979846.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2551..3492)
                     /gene="fabY"
                     /locus_tag="ISB17_001912"
                     /gene_synonym="yiiD"
     CDS             complement(2551..3492)
                     /gene="fabY"
                     /locus_tag="ISB17_001912"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAP1979847.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3537..3974)
                     /gene="dtd"
                     /locus_tag="ISB17_001913"
     CDS             complement(3537..3974)
                     /gene="dtd"
                     /locus_tag="ISB17_001913"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP1979848.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3971..4843)
                     /locus_tag="ISB17_001914"
     CDS             complement(3971..4843)
                     /locus_tag="ISB17_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAP1979849.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4837..5436)
                     /gene="yihX"
                     /locus_tag="ISB17_001915"
     CDS             complement(4837..5436)
                     /gene="yihX"
                     /locus_tag="ISB17_001915"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAP1979850.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLRAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5577..6464)
                     /locus_tag="ISB17_001916"
     CDS             complement(5577..6464)
                     /locus_tag="ISB17_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP1979851.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLTSPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6454..7803)
                     /locus_tag="ISB17_001917"
     CDS             complement(6454..7803)
                     /locus_tag="ISB17_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1979852.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7902..9335)
                     /locus_tag="ISB17_001918"
     CDS             complement(7902..9335)
                     /locus_tag="ISB17_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979853.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9405..10415)
                     /locus_tag="ISB17_001919"
     CDS             complement(9405..10415)
                     /locus_tag="ISB17_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP1979854.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10448..11335)
                     /locus_tag="ISB17_001920"
     CDS             complement(10448..11335)
                     /locus_tag="ISB17_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP1979855.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11360..12238)
                     /gene="yihU"
                     /locus_tag="ISB17_001921"
     CDS             complement(11360..12238)
                     /gene="yihU"
                     /locus_tag="ISB17_001921"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAP1979856.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12411..13307
                     /locus_tag="ISB17_001922"
     CDS             12411..13307
                     /locus_tag="ISB17_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1979857.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13347..14135
                     /locus_tag="ISB17_001923"
     CDS             13347..14135
                     /locus_tag="ISB17_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1979858.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14243..16066)
                     /gene="typA"
                     /locus_tag="ISB17_001924"
     CDS             complement(14243..16066)
                     /gene="typA"
                     /locus_tag="ISB17_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAP1979859.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            16439..17848
                     /gene="glnA"
                     /locus_tag="ISB17_001925"
     CDS             16439..17848
                     /gene="glnA"
                     /locus_tag="ISB17_001925"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAP1979860.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18022..19071
                     /locus_tag="ISB17_001926"
     CDS             18022..19071
                     /locus_tag="ISB17_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HAP1979861.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19083..20492
                     /gene="glnG"
                     /locus_tag="ISB17_001927"
     CDS             19083..20492
                     /gene="glnG"
                     /locus_tag="ISB17_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAP1979862.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            20604..20714
                     /gene="yshB"
                     /locus_tag="ISB17_001928"
     CDS             20604..20714
                     /gene="yshB"
                     /locus_tag="ISB17_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAP1979863.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(20947..22320)
                     /gene="hemN"
                     /locus_tag="ISB17_001929"
     CDS             complement(20947..22320)
                     /gene="hemN"
                     /locus_tag="ISB17_001929"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP1979864.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(22509..23018)
                     /gene="yihI"
                     /locus_tag="ISB17_001930"
     CDS             complement(22509..23018)
                     /gene="yihI"
                     /locus_tag="ISB17_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAP1979865.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            23600..24232
                     /locus_tag="ISB17_001931"
     CDS             23600..24232
                     /locus_tag="ISB17_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP1979866.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(24614..27400)
                     /gene="polA"
                     /locus_tag="ISB17_001932"
     CDS             complement(24614..27400)
                     /gene="polA"
                     /locus_tag="ISB17_001932"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP1979867.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            27402..27638
                     /locus_tag="ISB17_001933"
     CDS             27402..27638
                     /locus_tag="ISB17_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979868.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFDFHAVKNKAIP"
     gene            27758..28690
                     /locus_tag="ISB17_001934"
     CDS             27758..28690
                     /locus_tag="ISB17_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP1979869.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVRGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(28731..30161)
                     /locus_tag="ISB17_001935"
                     /pseudo
     CDS             complement(28731..30161)
                     /locus_tag="ISB17_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
BASE COUNT         7520 a         7802 c         7347 g         7579 t
ORIGIN      
        1 catcaggatg gtgtacccga aaaagtcagc agctttaccg caccaggggt taaggtgaaa
       61 tctaccattc ataacgaccg gtcagtgccg caccaaccgg tctttaattg ttagtagtag
      121 taaatttgtg gataacagtc cgggaaccag acgattcctt ccggatcatc cccctgcatt
      181 acccgatgac attggcgtcg atcatctgcg cgacggcgaa gcgtagtata gagccgcttc
      241 cattcatgat cgcccggtgt actgtagttt acccaacaat ccattgaagg gtgacgccat
      301 ttacctgtaa gtgttgggat ccctgaatgc ttatgcggct tcgctttacc attacgatcc
      361 ctatctttac gcgtattaaa cgtggacatt tttagtctcc tgttaagagt tctaaaaatg
      421 tgcttcctta aaatagttca tcattttccc ttaattcgca tagcgtgctt ttgcacgaat
      481 ttccgatacg cggggaccat tatgatagcg tcgatgccca cacatccaac aagaacaaat
      541 acagggagta cgaaagcaaa tacctgatgc ttttttgctt ccatcgcctg cagtgcgata
      601 acgacagcgg atttttttta atcgcaccat atgatggcga cgttcagcac gattacgggt
      661 catagcaaaa ctcctgaaca ggcggcacta tgccgctttc ataggagttc tgaatgctgg
      721 tgctttctgt atggcataac catgcctcta acggttaagt gcttgcagca catgttgcgg
      781 atcattggca atcgctcgca gcaaagcctt tgcaggcccg gtcggtgaac gtcgcccctg
      841 ctcccagtta cgcaacgtgt ctacgcttac ggaaataaga tcggcaaatt tagattgtga
      901 caaccctgaa gcgcggcgta tttctttgat tttcatagca tcaacttcaa aagtccgtga
      961 aggctgtcgc gttccttcaa taatttcatt catctgggcc atgctgtgag tcaaccgctc
     1021 aaaaagtgcc ttatccattt accacctcgt atttaacaac cggagcattg ctttttcctg
     1081 gggagacaga tcatctttaa tacccttacg ataaataagc agtaatctga tctcataatg
     1141 tttaacctga tgaaagtaaa taaccctgac accagcacgt ttcccctttc ctcctgagac
     1201 ccatcgaacc tttcttaacc caccagtttc aggtattacc tcaccatagt caggattcaa
     1261 ggccaggaaa aactgaaaac gactgaattc atcgtcgttc agcagttttt gaacatcttc
     1321 agtaaaaatt tccgtttcga tgaagagcat aattatacgc cattggctta gagcaacaat
     1381 cctttttgta cgtcattgac gtaccattga aaccactagc ttactgatag tgctccagca
     1441 aaaccacgtc tctgccgggt ttgtctttac atttgcgagg cgctttccag atttttcacg
     1501 cccgtgaatt tccctgtatc catccagcaa aaacgggtgt ataaaactgt acattcatac
     1561 agcaaaatga gttctactat ggcactggaa aaaggaattg cagagttagt cgaagagttt
     1621 atcgctgcgg gtcgcccctc atcgcgccaa caaagtatta cgcagcgcag agagggctat
     1681 atcgccagcg ctgtccttgc gggtgagaca gaaacgcggg tagatattca gactatcgaa
     1741 cttgaaggga tgaccttacg aatagtgtca ccgctgaatg ccccgacatt actcccgacg
     1801 ataatttatt actacggtgg ctgttttgtt agcggcgggt tcgcaactca cgacaaccag
     1861 ctgcgccagt tagcctatta cgggcaatgt cgggttattg ccgtccagta tcggctggca
     1921 ccggaacata ctttccccgc cgcccatgat gatgcacaac gaggtgcaga attagtccgg
     1981 caacacgccg aacgactcgg cgttgataaa caacgaataa ctcttgccgg tgacagtgcg
     2041 ggagggcatc tggcgctggt aactgctttg cgattgaagc gtgctggcga gtggcaacca
     2101 gcacaactca tcttaattta tcctatgctt gatgcgactg cgcatttcga aagctacata
     2161 cgcaatggtc acgattacat catcacacgc gacactttac tgagcggctt tgagatgtac
     2221 ctgccaggca ttgaacgcag gcatcctgaa gccagcccta tttggcgcaa tgatttcaat
     2281 ggcttgccac ctgtacatat cattaccgct gaatacgacc cactttgcga tgaaggtgaa
     2341 gcgttgtatc accgcatgac agggcaagga gtgcaatgca cctgccaacg ctaccttggc
     2401 gttatccacg gattttttca gttaggtggt atcagtgagg ctgcgcgatc ggcgttacgg
     2461 gatgttgcct ggagagcggg aaggtaactc cggaccaggg cccagaaaag gttttcagat
     2521 tgaaaacctt ttcagtcaag taaaacgccc ctactcttct tcgttcccgc cctcttcata
     2581 cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct tcaaacactg cacccggcgt
     2641 ctcgtcgcca aagatttcta cttgcatctg cacccgtgct tttcgtccgc gcgccagacg
     2701 gtcgagatcg ccacttaagg caccaaggtc ggctaccgca tggggttttc cgctaatcgg
     2761 cttgctgtag cggatatgcg catccgccag aataatcgtt ccgccgaggt ggcgttcgcg
     2821 cagcatcagc cagataagcc cccaaccggt gagcgtagcc agcgagaata aactcccggc
     2881 aaacagcgtg tgatgcggat tctgattgcc ggtttccggc atggtggtga taaatttttg
     2941 cccggtatat tgctgaatgc gcacacccat tttttcgctt aacgggatat gttcatacca
     3001 cgcttgctgg agctgggcgc accagtcgcc gcgatgaaga atatcatcca gagtggcgac
     3061 gggtttaatc atcagaaaat ggcgaatcgg cgtggtggtt ggcgtggtga tttcaccctg
     3121 attaacaaac cccagcttgg cgaaaaactc caccgcgtct tcacgggcgc tacaggtcac
     3181 gcgcttaacg ccttcctgac gtgccaccga ctccagggtc atcgccatca gcgtgcctaa
     3241 ccctttgtcc tgcacgtcgg gatgaacggc cataaagcga atggacgctt cattgtcggc
     3301 attaatatac agtcggccta ccgctaccag attaccctgc tcgtcgacga ccatctggtg
     3361 atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt ggttgatgca ggggcttacg
     3421 caacatttcc cagcgaaact gatagtaacg ctctaattct tcttctgttt gtggaacccg
     3481 aaggtgatac atagcgatac tctctcttgt ttcccgtgac aaccctggaa gctggctcat
     3541 acctgcaacc agaatgtcac ggggccatca ttgaccagcg atacctgcat atccgcagcg
     3601 aagcgtcctg tttgcgtgtt catatcttgc tgacggcagc gttcgacgaa atagtcatat
     3661 aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac ttgggcgcat cccccgttcg
     3721 gtatctgcgg caagggtaaa ctgggaaacc accagtacac tgccgcccgc ctgttgcacg
     3781 ttgagattca tcttgccttc ggcatcgcta aagatgcggt agccgagtac acgctcgcac
     3841 agacggtttg ctttctgttc gtcgtcatcc ttttcgacac ccaataacac caaaagtccc
     3901 gcgccaattt cacccgtcac ttctccctcc acggtgacgc tggcacgggt tacgcgttga
     3961 attaatgcaa tcatggttcg tcgtcttctt cttgttcagc agcttgtttt aatttgcggt
     4021 attccccgag agtgacagta atttccgcgc caagcaagac gatacaccat gtccagtaga
     4081 cccaaacaaa gagaatgggg atcaccgcca gcacgccgta aatgagctga tatgacggga
     4141 acatggtgat ataaagcgcg aaacctttct ttcctgcttc gaacaggagc gcggcgacaa
     4201 acgcgccgac aatcgcgtcg cggttaggta cgcgaatggt aggaacgatg ctgtacagca
     4261 accaaaagga gatccacgat aacagcagcg gaaaaatacg cagcacgttg tcgatgacag
     4321 tattgagatc gctcgcccag cgcagggaaa gcagatagga gctgatcgcc agactggccc
     4381 ctgccagcag cggccccagc gttaaaatca tccagtacac ggcaaacgag taaattttgg
     4441 gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct atcgatggag tacatcaaca
     4501 ataacgccgt gacgatcagc ccgcacgccc cgacggcggt cattttgttg gaattggcga
     4561 caaactgttc gatatatcgc tggataacat cgccagtagc aggcagaaag ttggcaaaaa
     4621 taaagtgacg caactgaatg ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa
     4681 aaacaacggc aaccagcggc actaatgaga gcaacgacac ataggcaagg ttacctgcca
     4741 gggttgtcat gttgtcctca tcaatgcgtt gccagagtag ttttagccag gcccatagtg
     4801 gacgggtacg atgcctggct ttgtcctgaa tggtttttag cataacacct tcgcgaaata
     4861 gtccgggatg gtggttttat ctttcaccag aatactagta atgcctagcc gattagctcc
     4921 ttctatatta tcggcgttat cgtcgaaaaa gaccgtatcg tctggtgaaa aaccttctgc
     4981 ccgcaaaaca tgctgataaa ttcgtgcttc aggtttgcgc atacccaagt cttgcgacag
     5041 atagatatgg tcagcagcat cacggatttc cgggtattcc tccggccaga aggtagtatg
     5101 caggcggtta gtattggaaa gcaccaccac gcgatgcccc tgctcacgca gtttatgcat
     5161 gatggcgatc acttccggtc gtagcgcaac aaataccgcc tgccaaccgt gggagaactg
     5221 ctcatagctt agcggcaggg ccatctcatg acacagtgcc tctgcgaacg cttcgtcgct
     5281 aatttcccca cgctcatgct gatgaaacgc ctcgcccatg tggaaactct tcttaagcgt
     5341 agccagtgga acacgcgtta aatcgctcca ggcccccagc acacggttaa agtcgatatc
     5401 gacaatcaca ttacctaaat caaagatata gagcattttt gcctcctttc gcgccacaag
     5461 aaattaactg tagcgggaaa ggatgggttt ggctatgtgg caataagggt aatcttgtga
     5521 tgctgattgc gaaaaagcag agtaatttca ctggcttttt cgcaatagat gaatggttaa
     5581 gattcaggtg taaattgcca acgagtttcg atgaccagcg gcttatctgg tgtcacaaat
     5641 tccggcgtta gatttattgc gttaggtgcc tgagtcagcg ggttaataca ggtggcatcg
     5701 ggctgtttat caaataccac cagactgttt gcagaagagg tcatggtcat cgcgagttta
     5761 ccaggccaca gtaacttaac cttcaccccg tcataaaaac caaaacagtc atcccaggga
     5821 ccaacttgag gagagatacg atttccagtt ggcagttcat cactacccgc ttcttcttgc
     5881 cagtccgcat caaaaagcac ctgcaacgat tctgtatttt gcggagtcag ctttttcgca
     5941 aaccacggat gccagcctgc ggaggccggg aatgtatcgg catatgaatg gacttccagt
     6001 tgcaaaacca gcgcatcgtt ctccagcaaa aaagtctgaa tcacttcccc ttgccagggc
     6061 cacggtgaag tcaggggcat acgtaaagtc agggaatctg ctgttttgtc gatgatttcc
     6121 cacgttgagt aacaagccat accatgcaac gcatgtggtg gtttattggc gggtaaagaa
     6181 taacattgcc caccagcatt cagcgtcgca ttccccagtc gccctgccca cggcaccatt
     6241 gggaaacagc catactgaaa agctctgcgc tgcggctgcc attggcgtaa tacttcagaa
     6301 ccaaatgcct ttaacgaggt tatcctggca ccatcatgcg gataaatctg caggtgaata
     6361 ttgccttttt ccagcgtcac taactctcct acaggcccag gccatggcgc gtcaaacgcc
     6421 tgttgtagcg ccaggtaatg ctcgtttttt cgttcagttt tcatgagatt gttccctgat
     6481 gtagcggtaa gaagcggttt cttgttcatc ggttggctca atcagccaga cgcaaacaaa
     6541 ggaaagaact gagacagcaa ataaaaacag agcgacatac cacggttttc ctccccctag
     6601 cgccagcaaa cttgaggcga taagggggct tagtccgcca agaatcgcgg aaaattgata
     6661 agcaaaagaa aggccggtat atcgtacttt ggtaccgaac atgcgggtga aaagtgttgg
     6721 ctgcacggca aacatcatcg tcggacctaa gttgtagcca aggatcatac tgcaccagat
     6781 gatgagcgta cttttgctat ccagtaataa gaagaaagga aacgcgaaca ggacacaaaa
     6841 ccctgctccc gacaaataca gacttttttg acctatacga tctgataacc atccaaccaa
     6901 tggacaactg aaaataccga tcgccgaggc aatcaacata cccgtcaggg gaatatctcg
     6961 cgacaatgct aattggctgg aaatatagac aataccaaag gcgttaataa tattagagga
     7021 gacactttcc gccagcctcg cacccagtgc caataaaata tttcgtggat gttttttgaa
     7081 taattcaatc aacggaggaa cggacttttc tttattattc gtcgtttttt gcttttgaaa
     7141 atccagcgtt tcttcggtat gcaggcggat aaacagccca acgatcaaca tcaccgcaga
     7201 aagccaaaac ggaatgcgcc atccccagga gagaaactgc tccgaagtaa gaaaatggtt
     7261 acagagagca aaaataccgg ttgccagcat aatacccaca gacgcagccg tttgcgggag
     7321 tgaacctaaa aatccgcgtc gggattcggg cgctgattct atggtcatca atgccgctcc
     7381 gccgtactcg ccaccaagac caaagccctg aattaaacgt agcaccatta atatgagcgg
     7441 tgcccagatc ccaatttcct gatacgtcgg aataaaacca atcaaaaatg tggagcaacc
     7501 gacaatagcc aacgtccaga taagcgttat ttttcgccct attttatcgc caaaatgacc
     7561 aaagacgatg ccacctaaag gacgagtcag aaaaccgaca ccaaaggtcg caaaggaagc
     7621 tataagacct ataaaaggat cgctgtctgg ataaaacagc ggtgcaaata ccagtcctgc
     7681 tgccgtgcca aaaataaaga agtcatacca ttccattaac gcgcctgcga aactgcccat
     7741 aacgacacgt ctcatggctt taccggaaag cccttcacgc ggtgcgggta acttttcaga
     7801 cataattttc ctctcctggt aggcgagaaa tctgggatac gaaccactaa acgtgggcag
     7861 tttgaattaa tgcgcatcgg cttaaaccga tgcgcattaa attatttctt caattcagcc
     7921 agaacctctg cgtcctcagc atcaacattt gcacccagtg gttcccaatt aatcaataca
     7981 caggtcagga atcccaggaa tggcacgatt gccaaccagt aaaacgcttc ggttttcaac
     8041 gcaccccaca gcatcggcca gaaaatgagt cctgcaattg cccctgtgcg cataatagcg
     8101 cgggcaaatc cgaccccagt agggcgaata gcgggaggat aagagagtgt ggcaatggtc
     8161 atgcccagcc caccaggacc cgctgaatgg aagaaaataa ttgatcccaa taaccctaaa
     8221 gacagccagg gattagtcgt tgccagtgcg cctaacgaca gcaatgctaa ggtcacaaca
     8281 gcaaatccat acatcgactg tcggcgagta cccagtcgtt ggagaataaa cgagccgatc
     8341 atcccgcctg tcaccccaca aagggcattc acgacggcag aaccggtcaa cgcgccagta
     8401 agcccgccac ttattattcc cgccaacgta agaggaagat aaacacctac ggcgttatat
     8461 tgccacgcct gcatggttgc cacgacacag ccaagaatgg tgcgtttgcg gtaacgatca
     8521 ttaaataaga tgccatatcc accactgtat ttattctctg ctttttccga gaatttcgct
     8581 tctgttgtac ccgatgccgc aacgcgagcc tgaacaccat aacgttttcc cagaatgtcg
     8641 cacgcttcct ggtaacgccc gacgcgtgcg gcccacattg cggattcacc aataaagaag
     8701 taacgcaaaa tcatgaagat gactgcaaaa atagctccga tgaatattcc gtaacgccac
     8761 aattgttctt cagcgatacc cgacagaagc agcggcaaaa gcaccaaata aacaacggtt
     8821 gtcgaaacat accaggccat ttgccagagg ttgaccgacg ttccggtttt cttcgacgtt
     8881 gaaccacgaa gctctgccac ggcattggtt gccaaaggga agtcgatgcc taaaccaaag
     8941 cccatgatga cgcggcacac cagcacccac cagatactgg gagcaatagc taccgctgca
     9001 gcaccgatgg tgcacagccc catgccaata ataaatgccc gcttttgccc aaagcggttt
     9061 attactcggt tcgctaataa accgccaatt aacgcggcga ttaatactga tgcattgacg
     9121 gttgcagcaa gacccggcga aatgccgaat tgagcggcaa tgtatttatt accaaacccc
     9181 accatcgccg cctggtaggc atcaatcaat atgccgccca aagcaataaa aataacgccg
     9241 atactggtgt tgattgcaga gttcttatta acaatatcaa ttacgtcctg tggccgacta
     9301 attgaataag ttgcaggact ggtgatttga ctcatcgcag tgtccttttt aattgttgta
     9361 gggtggcgct gacgtgacgc agcgccagac agagatttag aaaatcattc cggcgcaaat
     9421 tgcagcatga cctttatgga gtcattttgc tgcgcgcgtt cgaatgccgc cgctgcatcc
     9481 tcaatggcaa ataaatcggt aaccagttca ctggtatcaa accgcccatc cgccagccat
     9541 tcgataacag catcgaaatc ttcagggatg tacatgccag agttgagcaa gtcacgctcg
     9601 aaacgttgca tcatcggtaa tggtatttca cgcggcccac ttggtacgcc catgcacacg
     9661 acggtgcctc cggcatatac acgggtacat gcatcgttaa gcgtggcctg cgctgcaacg
     9721 acatcaatcg cccccgtaaa tcgcacatct gcggccagct cacctggtgc ccaaacttct
     9781 gctgcgccca gttttaacgc cagcgcgcgt ttggcagcgt caggttcaat cacggtaatc
     9841 ttacctgccc ccataatgcg cagtgcctgc acgatggata agcctatcgt tccggcacca
     9901 atcaccagta cgctttccag agaagctttc ggcattcggt taacgcagtg acgagcacaa
     9961 gccgccggtt cggcaaaggc caacactttt agcggtaaag aggctggtgc gacatggcaa
    10021 ttacgcgcag gaacaatgtg ttgtgagcga gcaaagcccg gtgcgcgaaa accagcaacc
    10081 tgcggtggtt cacaaagatt aaaacgtcct gctttgcagg ctcggcattc catgcaagcc
    10141 atgatgggat cgaccacaac atgatcgccc ggctgtacat ttttaacgtc gctgcccact
    10201 tccgtaacgc gcgctgcaat ttcatgaccg gggacgacag gcggtttggc aaacggatga
    10261 ccgcctttca gaacatgcag atccgaaccg catatcccgt agaacacggg cgcaatccgt
    10321 acttcgtgcg gtttaagttg ctggtgtggt acttcaacat attgagtggt gactttccca
    10381 atatctgaaa ataaaacctg ggtaatttta tccattgtta ttctccagaa tcgatatcca
    10441 taggggatta gcgttgcgcg ataccttcat cgacaatttc agccagccgt tgcaagcgtg
    10501 gaacggagac atcgcgcagc atggtttgtg gatcttgtgc accaacaacc gacgcccaaa
    10561 ccgctcgtcc agccaggaag cccgacgcac cacctttcat ggcaatactt actgcacggc
    10621 caaaaatatc ggcatcaacg ccagaagaaa gaattaccca cggcatcttc atctgatcgt
    10681 taagttgctg acaggcagca agtaaggttt tttcatcgcc tttgccccct aatggcatct
    10741 cggctttgta aagatcagcc tctgttccac caagctctgc ggcggcggca acaatggcgc
    10801 tttcacgatc aaagtcccaa ccgcgacgag gtggacgcac cacaggttca ataatgctaa
    10861 ccagcccggc actacggcag cgacgaacaa atttatcaac cattgccaga cgttcctctg
    10921 cgggttcatc ttcacgccag agtacaagta atttcattgc cttagccccc atttcacggg
    10981 ctttatgcgg gtcgacggac atatctattt caacgctatc aaccgggatg ccattacctg
    11041 ggataaaaag atcggcagcg acaatcaggc cgcaagaatt agccacagcg ccctgctcga
    11101 ctatttgttc aagacaaaat tgtttatccg ccagaacggc agaagcataa ggagaaagga
    11161 ttcgggtcgc ggcaacttta aaatcggtga gtacactgtc ggcgatgggt tttggctgcc
    11221 ctgctgccgc aaacatcagg cgcatggcct cacgctgatc gacagctaac atggcgaatc
    11281 caccacctgg gcgggagata tctttcagcg taaatggggt gaagtgggat gacatagaaa
    11341 ctcctgttaa tagataaaat taaagaccag cctgctggcg aacctgattt agcagggcgc
    11401 tccagtcctg ccgccctttg ccgtcttgac ttgctgcggc gtaataactt tctgctgcaa
    11461 tccccatatt gagcggcgcc cccgttttcc gagcgacgtc aactgcaata cgcagatctt
    11521 tcaatgccag gtccaccatg aaggcgggag aaagatcgcc tttcagcact ttgccaggcc
    11581 aggtggtcgt aaaatgtcct ttccctgctg ccgtaccact catcacctta attgcgacat
    11641 caagatttaa tcccagtgac tcacataaca cagcggcttc ggaggacagc gcattaagcg
    11701 caatgctcat atagttgtta attagcttca cgcggatccc cattcctggg ccaccagcct
    11761 cgaccagctc attacccatg cacatgagaa tcgggcgtgc acgcgcaatc tgctcatcag
    11821 tgccgcccgc taaaatcagc aatgtgccat caatcgcatt cactgacgtt ctgcccacgg
    11881 gggcgtccat catattgatg cctttttctt gtaattcccg tattaatgca tcggtctgta
    11941 agggatgaat agtcgacata tcaatcacta acgcgtcaga tgaaacagtt tcacagacgc
    12001 ctttttcccc caacagcacc tgtcgaacaa tgtccccatt gggaagcata gtgatgataa
    12061 attcagcgct tgctgccgct tccgctgggg tttgtgccgc agtggccccc tgcgtaacta
    12121 gcgcatcgac ggcctgtgta ttgacatcaa atacctgtaa tgaatgccct ttttgcaata
    12181 gattgttagc cattgggcta cccatctgcc ccagcccgag aaatgcaatg gttgccatga
    12241 atactctcct gtctgacatt gacaaaataa tgacaattga aaattcgttt tcgtctgttt
    12301 ttgagcatat ttgtaattta tgttcaaaaa cacgacacgc atcactaatt tgatcatttg
    12361 tgtcatttaa attgaccaaa gacgacaaaa ttaaacagga caagacaaat atgaccagaa
    12421 ttgcatgcgt gggtatcaca gttcaggaca gaatttattc tttgcccact ctgccggagg
    12481 gtggcgggaa atatcaggca aatcattatc ttgagattgg tggcggcccg gcagcaacag
    12541 ccgctgttgc aatcgcaaag cttggcgtgg aggttgattt catcggtcgc gtcggcgatg
    12601 acagttgtgg aaatacctta cttgcagaac ttgagggttg gggcgtcaat accgcatttt
    12661 gccgccgata tcctaacgcc cgttcgtcgc aatcagcgat cctcgttgat cagcatggcg
    12721 aacggattat cgttaattat ccaagtcctg atctcggtac tgatgccgaa tggttagaag
    12781 cgatcgattt ctctcgttat gatctgattc tggccgatgt tcgctggcat agcggtactg
    12841 aaaaagcatt ctcgctagcg cgccttgcgg gtgtgacaac gcttttagac gccgatatga
    12901 caccgcagga tatttctcca cttgtcgcgc ttgccgatca cgccgtgttc tcaacaccag
    12961 ggctgaaacg catgactggc ctgcagtcac ctgaagaagg tttgttccag gcaacgacac
    13021 aaacagcagg taaagtgtat gttacgctgg gttctgaagg atcgttgtgg attgaagatg
    13081 gtcatttgtg tcaacaggag gcattttctg tcaatgtggt tgataccact ggagcaggcg
    13141 atgtttttca cggcgcactc gcggtagcgt tggcagaaaa aatgccaaca aaagaggcta
    13201 ttcgttttgc cagtgcggtt gccgcgatga aatgtaccca gcctggcggt cgggccggca
    13261 ttcctaatcg tgagcaaacc gaatcatttt tgtcacttta tgcgtaaaat ggtaaacatc
    13321 taccgaggag gggcaaggga gtattcatga gtatcatcga agtgacaggt aatccacgcc
    13381 atgaccaact cgttcatcta atcgcagaac gcggttatat gaatattgaa gaactggcac
    13441 aactgctgga tgtttcaacg caaaccgtgc gccgggatat ccgaaaactc agtgagcagg
    13501 gcttgatcac ccgtcatcat ggaggggccg ggcgcgtctc cagcgtcatg aataccgctt
    13561 ttgagcaacg ggaactttcg ctcaccgccg agaaacgggc gatcgctgag gcagtcgccg
    13621 attaccttcc cgaacgctgt accgtcttta tcaccatcgg tacaaccgta gaagccgttg
    13681 ccagggcatt actcaaccgg cgtgatttac gcattatcac taatagcctg cgtgtggcac
    13741 agattcttta taagaatcag gatattgaag tgatggtgcc gggaggcact ttacgcgctc
    13801 ataacggtgg gattatcggc ccaggagccg tggattttat tgaaggcttc cgcgcagatt
    13861 atttaatcac cagtataggt gccattgaac atgacggcac cctactggaa tttgatgtca
    13921 atgaagcgtt agtcgcgaga acgatgatta aacatgcgcg gaatacattg ttagtggccg
    13981 atcatacgaa gtttaccgcg tctgctgccg tttcaattgg caatgcacgg aatgtcaggg
    14041 ctttttttac tgatgccccg cctcccaatt ctttctgcca gttgttaagt gaagagaatg
    14101 ttgaactggt ggttgccgag caagaagtat cctgaaattg aaacttcgga aaatccgctg
    14161 ggaatttcag cggattttgt tttccataag ccaaaaaaaa acctgccatc gctggcaggt
    14221 tttttatgac tgaaaaacga aattaatcgt ctttcggtgc gcggttagcg cgacgacgat
    14281 cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggagtcact tctaccagtt
    14341 cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa
    14401 caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt
    14461 ttacagtcag gtcgttagag cggctgtgaa taccgataat ctgaccttcg taaacttctg
    14521 caccgtgacc gaggaacagc ttaccgcggt cctgcagacc gaacagtgcg aacgcgaccg
    14581 ctttaccctg accgttagag atcagtacgc cgttctgacg ctggcccact tcacccggac
    14641 gtacgtcgtc gtagtggctg aaggtggagt acagcagacc agtaccggaa gtcatggtca
    14701 tgaactcaga acggaagcca atcagaccgc ggcttgggat cacgtagtcg agacgtacac
    14761 ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca
    14821 ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac gttttcatac ggctcttgtt
    14881 tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt tcgaaacctt
    14941 cacgacgcat gttttcgatc agaacagaca ggtgcagttc gccacgacca gaaacgcgga
    15001 acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt
    15061 tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg
    15121 aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg
    15181 cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgccc aggcccgtga
    15241 tcgccacgat atcgccagct tccgccagat cggtttcgat acgttccaga ccgaggtggc
    15301 ccagcacttt accgactttc gcgttgcggg ttttgccttc gctatcgatg atagtaacct
    15361 gttggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc
    15421 tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg
    15481 caggaacgtg gtcaacaatc gcctggtaca gcggggtcat gtcttccgcc atatcttcgt
    15541 ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag tccagctgct
    15601 cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac
    15661 gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg
    15721 cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca
    15781 gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg
    15841 gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga
    15901 tggtaatccc acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg
    15961 cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat
    16021 ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg
    16081 cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac
    16141 atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag
    16201 ggttatccaa aggtcattgc accaatatgg tgcttaatgt tcccattgaa gcactatatt
    16261 ggtgcaacat tcacatcatg gtgcagccct tttgcacgat gttaagcatg ataacgcctt
    16321 ttaggggcaa tttaaaagtt ggcacagttt tcgctttatc ttttttacgg cgacacggcc
    16381 catttaattg cagatttcgt taccacgacg acaatgacca atccaggaga gttaaagtat
    16441 gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgacttgcg
    16501 cttcaccgat accaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc
    16561 cgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat
    16621 taacgaatcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt
    16681 cgccgattct accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta
    16741 tgaccgtgac ccgcgctcta ttgcgaagcg cgctgaagat tacctgcgtt ctactggcat
    16801 tgccgacacc gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt
    16861 cggatcgtct atctccggtt cccacgttgc tatcgatgat atcgaaggtg catggaactc
    16921 ctccacccaa tatgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt
    16981 cccggttccg ccggtagact cagctcagga tatccgttct gaaatgtgcc tggtgatgga
    17041 acagatgggt ctggtggttg aagctcatca ccacgaagtc gctaccgcag gtcagaacga
    17101 agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata
    17161 tgtcgtgcac aacgtggcgc accgtttcgg taagaccgcg acctttatgc caaaaccgat
    17221 gttcggtgat aacggttccg gtatgcactg ccacatgtcg ctgtctaaaa acggcgtcaa
    17281 cctgtttgct ggcgacaaat acgcaggtct gtctgaacag gcgctgtact acattggcgg
    17341 cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac ccgaccacca actcttacaa
    17401 gcgtctggtc ccgggctacg aagcaccggt aatgctggct tactctgcgc gtaaccgttc
    17461 tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt
    17521 cccggacccg gcggctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga
    17581 tggcatcaag aacaagatcc atccgggcga agccatggac aaaaacctgt atgacctgcc
    17641 gccagaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag cactgaacga
    17701 actggatctg gatcgcgagt tcctgaaagc cggtggcgtg ttcacggatg aagcgatcga
    17761 tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt
    17821 agagtttgag ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg
    17881 ccgggattta gttgccgtgg aaactttcag cccatctctg catgggcttt tttctccgtc
    17941 aattctctga tgcttcgcgc tttttatccg taaaaagcta taatgcacta aaatggtgca
    18001 acctgttcag gagactgctt tatggcaaca ggcacgcagc ccgatgctgg gcagatcctt
    18061 aactcgctga ttaacagtat tttgttaatc gatgacaacc tggcgatcca ttacgccaac
    18121 cccgccgcgc aacaactgct cgcccaaagc tcccgcaaat tgtttggtac accgttaccg
    18181 gaactgttga gctacttctc attaaatatc gagctgatgc aagaaagtct ggaggcgggg
    18241 caaggtttta ccgataacga agtgacgctg gtcatcgacg ggcgctcgca tatcctttct
    18301 gtgacggctc agcgtatgcc ggacggcatg atcctgctgg agatggcacc gatggataac
    18361 cagcgccgct taagtcagga acagctacag cacgcccagc aggttgctgc ccgtgattta
    18421 gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag
    18481 ctgctcagca aagcattacc tgatccgtca ctactcgaat ataccaaagt gattatcgaa
    18541 caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg ggccgcagct gcccggtacg
    18601 cgcattaccg aaagtattca caaagtggct gaacgcgtgg tgacgctggt gtcgatggaa
    18661 ctgccgaaca acgtgcggtt gattcgggat tacgacccca gcctgccgga actggcgcac
    18721 gacccggatc aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct acaggcgctg
    18781 gggccggagg gtggtgaaat cattctacgt acccgcacag cgtttcaact gaccttacac
    18841 ggcgagcgct atcggctggc ggcgcggatt gatgtggaag ataacgggcc gggcattccg
    18901 cctcatttgc aggatacgct gttttacccg atggtcagcg gccgcgaagg tggcaccggg
    18961 cttggcttat ccatcgcccg taatttgatt gatcagcatt caggcaaaat tgaatttacc
    19021 agttggccag gtcataccga gttctcggtt tacctgccta tcaggaaata aaggtgacgt
    19081 ttatgcaacg agggatagtc tgggtagtcg atgacgatag ttccatccgt tgggtgcttg
    19141 aacgtgcgct cgctggagcg ggtttaacct gtacgacatt tgagaacggc gcggaagtac
    19201 tggaggcgct ggcgagcaaa acgccggatg tgctgctttc agatatccgt atgccgggaa
    19261 tggacgggct ggcgctgctc aagcagatta aacagcgcca tccgatgctt ccggtcatca
    19321 ttatgaccgc acattccgat ctggatgctg ccgtcagcgc ctatcaacaa ggggcatttg
    19381 attatctgcc caaaccgttt gatatcgacg aagccgtggc gctggttgag cgcgccatca
    19441 gtcattacca ggaacagcag cagccgcgta atgttcagct taacggccca acgaccgata
    19501 tcatcggtga agcgccagcc atgcaggacg tgttccggat tatcggtcgg ctttcgcgtt
    19561 cttctattag cgtgctgatt aacggcgaat ccggcaccgg taaagaactg gtcgctcatg
    19621 ccctgcatcg ccacagtccg cgagccaaag cgccgtttat cgcgctcaat atggcggcta
    19681 tcccgaagga tttgatcgaa tccgaactgt ttggtcacga gaaaggcgca tttaccggcg
    19741 cgaataccat tcgtcagggg cgttttgaac aggctgatgg cggcacgtta tttctcgatg
    19801 aaattggcga tatgccgcta gatgtgcaga cgcgtttgct gcgcgtgctg gcagacggtc
    19861 agttttatcg cgttggcggc tatgcgccgg tgaaagtaga tgtgcggatt atcgctgcca
    19921 ctcaccagaa tcttgaacag cgggtgcagg aaggcaagtt ccgtgaggat ctgttccacc
    19981 gcctgaacgt catccgcgtt catctgccgc cgctgcgcga acgtcgggaa gatattcccc
    20041 gtctggcgcg ccatttttta caggttgccg cgcgagagct gggcgtagaa gcgaagttgc
    20101 tgcatccgga aaccgaagcc gcgctgacgc gtctggcgtg gccaggcaac gtgcgccagc
    20161 tggaaaacac ctgtcgctgg ctaacggtga tggctgccgg gcaggaagtg ttgattcagg
    20221 atttgcctgg cgaactgttt gaatcaaacg taccggaaag cacttcacac atgcagccgg
    20281 acagttgggc aacgctgtta gcacagtggg cagacagagc gctgcgttcc ggtcatcaaa
    20341 acctgctttc cgaagcacag ccagagctgg aacggacgtt actgaccacc gcgctgcgac
    20401 atacgcaggg gcataaacag gaagcggcgc ggctacttgg ctggggtcgc aacaccctga
    20461 cgcgtaagtt aaaagagctg gggatggagt gattcacggc ttgtgtgtaa agattgatta
    20521 ttgagcgcaa attgctggta ttttgcgctt tactgttccg ataagttcag tatgatcttg
    20581 cccggaaaac ggggagagtc attatgctgg aatcaataat taatctggta tcgagtggcg
    20641 cagttgacag ccacacaccg caaactgctg ttgctgccgt gctgtgtgcc gcgatgatag
    20701 ggctgtttag ctgaagctcg ttgtttacgc cggatgcgga gaaagtcgta ggcctgataa
    20761 gacgcaacca gcgtcgcatc aggcgttgat tgccggatgc ggcgtaaacg ccttatccgg
    20821 cctacaaaag caataatttc aacagatttt gcaggcctga taagcgtagc gcatcaggca
    20881 atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg aagcgccatt
    20941 cacaatttaa atcacccgag agaactgctg catccgcgct ttctggcgca gataggtatc
    21001 aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct gtattccctt
    21061 ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga gatcttccgc
    21121 aaagtaatca gcgaagagca aatcccactg ttgctcaata ggggcgtaat ccagacggaa
    21181 gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat
    21241 accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct tcaactcttt
    21301 ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca tccccagcag
    21361 atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc cttcacgctg
    21421 ggccaccgcc agctcgtcat ccggacgggc aaagtgatcc ataccgataa actgatagcc
    21481 cgattgtgtc aggaaggcga tggtttcctg caggatatcg agtttttgct gcggactcgg
    21541 caggtcagca tctttgattt tgcgctgggc ggcaaaaatg gtcggcaaat gcgcgtagtt
    21601 aaagacgctc agacggtcag ggttcagttc cgccacacgc ttcagggtaa aggcgaaact
    21661 ctccggcgtc tgtttcggca ggccgtaaat caggtcaatg ttggtggagg taaagccaat
    21721 ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc ggttaaccag
    21781 acgttgcact tctttgttga agtcctgcac gcccatgctc agccgattaa agccttcggc
    21841 gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga tcgagatctc
    21901 cgcatcggca ttgaactgga agttttcgcg cagcagcttc atcagacggc tgatttgcgc
    21961 tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc
    22021 gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact gatcggcctt
    22081 gtgctgctgg cgagtaacaa tcttattgca accgcagaag taacaaagct tatggcagaa
    22141 cggaatatgt acatagagag ataatggacg ctcaggatag cgcgccacag cttgtaaaaa
    22201 cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg
    22261 cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct gtacagacat
    22321 gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg cccgtttcga
    22381 gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag tttaacgaat
    22441 aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag gcccacggcg
    22501 gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc gtcttcttcc
    22561 tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc aatacgatcc
    22621 agtttggcat ccacccagga ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt
    22681 tccagcagcg catccagacg ctcatccgtt tccagtaact ccaactccgc ctgcggtgaa
    22741 agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt cacgcccaat
    22801 ggaatagggg ttttactgcc aatacgtgga tcttttggtg cgttctgacc tttgctgcct
    22861 gacgtggtgt taccgcccgc tgcgcggctg cccggcgcat gaccacgacg ttttttctga
    22921 cgcttacggt cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca
    22981 tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct tttagccgtt
    23041 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    23101 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    23161 ggccgccaac acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttcca
    23221 tttaattacg tctccggacg tttatcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    23281 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    23341 tacgcctttg ctttaaataa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    23401 tatataaaag caattcactg atttatatag cttatttgac atacgtcgca gaattttctc
    23461 tgctatttcg cataaagact atcggcaatg tctcacaaag catatggctt ttacttacaa
    23521 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgccg caaagctttg
    23581 actgatggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    23641 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    23701 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    23761 tacctcaaaa accccagggc gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    23821 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    23881 atggcagcgc gcgctcggcg agtacctcga aaaacgtcag agcctgcaag gtctggtggt
    23941 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    24001 agacagcaat atcgccgtgc tggtactgct gaccaaagcg gacaaactgg caagcggcgc
    24061 acgtaaagcg caattgaata tggtgcgtga agcggtgctg gcgtttaacg gtgatgtgca
    24121 ggttgaaacg ttttcttcgc tgaagaaaca aggcgtggac aagctgcggc agaaactgga
    24181 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    24241 ccttaatgct tgtgccggat gtggcgtatc cggcccgtaa attcaataga ttacaattct
    24301 accgttggcc tgataaacag ggcgcatcag gcattacgga tcttttcttt cgcccaataa
    24361 aaaacgcccc agtcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt
    24421 gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac
    24481 tcttttttgt tcagttcaga aagcattttt tgtaattttt tttcagttgt tgcataggaa
    24541 tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat
    24601 gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca
    24661 acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat
    24721 cgacatcatc tttatggact tcaaatacca gttcatcgtg tacctgcatg atcatacgca
    24781 cacgcggttg ctcagcctgt agccacgcat caacggcaat catcgcccgt ttgataatgt
    24841 cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag
    24901 ccccattgct ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt
    24961 agccctgctc tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc
    25021 gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg
    25081 ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacgac
    25141 gttgctcgct ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcacggt
    25201 ggatatcttt tccttccgcg aatgcggtca gcaagccttt gtcacgcgag agatgcgcca
    25261 taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa
    25321 taaacgcctg gcggatacgg cgaccttctt cgttacgcac cggaatgttt tgcaggttag
    25381 gatcggttga cgataaacgt cccgttgcgg ttactgcctg gtgataagag gtatgtacac
    25441 gcccggtttt cgggttgatc atcaacggca gcttgtcggt gtaggtcgat ttcagcttcg
    25501 ccagaccacg atattccaga atcacttttg gcaacggata gtccagcgcc agttcttcca
    25561 gtacctcttc cgacgttgac ggcgcgccac ccggagtttt cttcagcggt ttaatgccct
    25621 gtttttcaaa cagaatggtt tgtaactgct tggtggaaga aaggttaaat tcttcacctg
    25681 caatttcatg cgctttcttt tccagctcag ccagacgcaa ggtgagctct tcagagtgat
    25741 tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca
    25801 ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg
    25861 gccacatttt cagatgcaac tgcaaggtga catctgcatc ttcggcggcg taacgtccgg
    25921 cttcttcgag ggcaatctgg ttaaaggtca gttgattttt tcctttacca gcaatctctt
    25981 caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc
    26041 cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagct
    26101 caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg
    26161 ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgcga gagatttgat
    26221 cgggcgcatc aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag
    26281 caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa
    26341 atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa
    26401 ggatggtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc gcttcgtctg
    26461 caacactggt ttcctgcggc ctggctgcgg gtttcgcccc tttagcctgt agccatttgc
    26521 ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc cctaacaact
    26581 cttccgccgc cggtggctgc acttccagtt gttcacaggt cagctccagt tcaacgtcgg
    26641 ttttaatcgt cgccagctgg tatgagagat aagcaacttc tttgttttgc tcgagcttgg
    26701 ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc tcggcataca
    26761 gcgtatccag cccgccaaga ccttgcagca atgcctgcgc ggttttttca ccgacgcccg
    26821 gtacgccagg gatgttatcg gaggagtcac ccatcagcgc caggaaatcg atgatcagtt
    26881 ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg
    26941 tgttgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctga
    27001 tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg
    27061 cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcagag
    27121 gttcgatttg tgctcgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa
    27181 acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt
    27241 tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct
    27301 cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc gcgataaaga taagatgaac
    27361 catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc
    27421 agattatggg taaaggatgc cacagacagg atgaaaacat gagtttttca ccgcttttcg
    27481 tgaaaatctt ttgtagatct tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc
    27541 ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg atgtttgtct
    27601 ttgattttca tgcagttaaa aataaagcca tcccttgaga taatgggatt ttttctaatg
    27661 tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt cgttttctgc
    27721 taaaatctgc gccttgcgct tctttagcgc actgtctatg gctaaccttt tgaataaatt
    27781 cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat tgaccatttt
    27841 ggtcactatt ttttgctccg tcccgatcat cattgccggg attgtaaaac tattgcttcc
    27901 tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt attgctggtg
    27961 tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag ttcgcgggct
    28021 ggaagggtta agtaagaaaa actggtatct gcttatttgt aaccaccgta gttgggcaga
    28081 tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat atttcctcaa
    28141 gcagcagctg gcctgggtgc cttttcttgg cctggcatgc tgggcgctgg atatgccgtt
    28201 tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagagacgtg gtaaagatgt
    28261 tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca ttgttaattt
    28321 cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acgcactcca cttttcaaaa
    28381 cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg gtaaacaatt
    28441 cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc cgttcttcga
    28501 tatgttaagc ggtaaactga cgcgaattgt cgtacatgtg gatttgcagc ctattgccga
    28561 tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc agcaatggct
    28621 gaactcactg tggcaggaaa aagaccggct tctgacgtcg ttgatgtcat cacagcggca
    28681 ggacaagtaa attttgctcg cctctgaatg agcagaggcg agtgagtatt ttaatgaaca
    28741 aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt aaaaacaact
    28801 ttacccggtg tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt gattaactgc
    28861 aacatctgac ctaaggtcat catcccgctg atttgtttat cagactgctt ttgcgctttt
    28921 tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtggc gacattaagc
    28981 ggtaatttca catcaaaatt gagcgattta atatctttgt ttgtggatga tgaagatttg
    29041 gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc attcttccat
    29101 cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata ctctttgaac
    29161 aatgcagtat tcacttcttg cagagcaact tcatcgtttg ctaactcagg gtgtgcagca
    29221 agctgcttct gcatcgcaat gttatactgg atgataaatt ggcgcaccgc tgagggatca
    29281 atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc ttttgttaac
    29341 ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac gcgatccagc
    29401 ttggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt gataatgtta
    29461 atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc taatcgcgcc
    29521 agagaatcaa tggtaaaact tttcgccgtt actttgttca ttggtgataa ttgcagaaca
    29581 atatcttcaa ctttgccttc aatatccagg ttagacaatg aactatcatc accattaaaa
    29641 gtaaactggc cttcaccaaa agaaaacttt ccatactctg caggggcaac atctaatgtc
    29701 gtggagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga tttatttttc
    29761 gcggcgataa atagcggttg tgtcagctca ttattcacta agttcacttt tgccgctgcc
    29821 agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc gtgctcaaca
    29881 tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc accgttatca
    29941 aatgtcattt gcatttgaaa acgggaatta aaaaaacctt tgtcaaaatt tttataactt
    30001 aacgtcatgt cgtaggcatg ttcacctttc tatttagcat cgttaaaatc tttaataaac
    30061 ttttcgacac ctggctgaat ttgcgtacct gtgtaccatg tgccaccacc ccagattacg
    30121 gataacgcaa caatggcacc tgtagctgac ttacgtatca tataaatatt ccctgataaa
    30181 acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcagcag gacgcattat
    30241 cagaatgt
//
LOCUS       DACADA010000043        30226 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.043, whole genome shotgun sequence.
ACCESSION   DACADA010000043 DACADA010000000
VERSION     DACADA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.043"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            192..2864
                     /gene="rcsD"
                     /locus_tag="ISB17_001936"
     CDS             192..2864
                     /gene="rcsD"
                     /locus_tag="ISB17_001936"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAP1979870.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2881..3531
                     /gene="rcsB"
                     /locus_tag="ISB17_001937"
     CDS             2881..3531
                     /gene="rcsB"
                     /locus_tag="ISB17_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAP1979871.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3731..6580)
                     /gene="rcsC"
                     /locus_tag="ISB17_001938"
     CDS             complement(3731..6580)
                     /gene="rcsC"
                     /locus_tag="ISB17_001938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAP1979872.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            6747..8573
                     /gene="atoS"
                     /locus_tag="ISB17_001939"
     CDS             6747..8573
                     /gene="atoS"
                     /locus_tag="ISB17_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HAP1979873.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8570..9955
                     /gene="atoC"
                     /locus_tag="ISB17_001940"
     CDS             8570..9955
                     /gene="atoC"
                     /locus_tag="ISB17_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HAP1979874.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10151..10813
                     /gene="atoD"
                     /locus_tag="ISB17_001941"
     CDS             10151..10813
                     /gene="atoD"
                     /locus_tag="ISB17_001941"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HAP1979875.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10813..11463
                     /locus_tag="ISB17_001942"
     CDS             10813..11463
                     /locus_tag="ISB17_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HAP1979876.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11460..12782
                     /locus_tag="ISB17_001943"
     CDS             11460..12782
                     /locus_tag="ISB17_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HAP1979877.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12813..13997
                     /locus_tag="ISB17_001944"
     CDS             12813..13997
                     /locus_tag="ISB17_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP1979878.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14071..14847)
                     /locus_tag="ISB17_001945"
     CDS             complement(14071..14847)
                     /locus_tag="ISB17_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAP1979879.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14852..16501)
                     /locus_tag="ISB17_001946"
     CDS             complement(14852..16501)
                     /locus_tag="ISB17_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAP1979880.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKLGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16502..20896)
                     /locus_tag="ISB17_001947"
     CDS             complement(16502..20896)
                     /locus_tag="ISB17_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAP1979881.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKP
                     SRYVQNSQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPL
                     GKQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDGSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTG
                     EAVSGKLQLLYPDGEPVKDARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDN
                     GHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGE
                     SVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDK
                     DGLILAGLSHAVSGKDSTSHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLY
                     MKWSMPTVYRMGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTS
                     DINDSMILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAK
                     HKLTGGGEYWRWVGQGVPDILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAGKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAPGSEWIGMQVK"
     gene            complement(21040..21663)
                     /locus_tag="ISB17_001948"
     CDS             complement(21040..21663)
                     /locus_tag="ISB17_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAP1979882.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21660..23348)
                     /locus_tag="ISB17_001949"
     CDS             complement(21660..23348)
                     /locus_tag="ISB17_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAP1979883.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(23497..26124)
                     /gene="gyrA"
                     /locus_tag="ISB17_001950"
     CDS             complement(23497..26124)
                     /gene="gyrA"
                     /locus_tag="ISB17_001950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAP1979884.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            26271..26993
                     /locus_tag="ISB17_001951"
     CDS             26271..26993
                     /locus_tag="ISB17_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAP1979885.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(27133..>30226)
                     /gene="yfaL"
                     /locus_tag="ISB17_001952"
     CDS             complement(27133..>30226)
                     /gene="yfaL"
                     /locus_tag="ISB17_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAP1979886.1"
                     /translation="KTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNN
                     GVMIDENNSASGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAG
                     TGVIVKEGAGELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGL
                     MVGSTVHSEYQAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEG
                     QLTVAQDGSYLLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVL
                     DLSDFTTWDGDSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVI
                     NAGDQRVTLANNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTT
                     DVDTRSATTGQGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGT
                     LELTASGTATSAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSG
                     GDIDITDGTVLRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYL
                     SDVTVNGNLTNTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLN
                     GNTAGNTTVVINPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDY
                     TLVEDNNDWYLRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGG
                     YFNNLRAANQAFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGN
                     LFSGHWGDDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQK
                     QGAWLDSWLQYAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVI
                     YQGVQQDDFTAANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEI
                     EEDGSTISDDAVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW
                     "
BASE COUNT         7255 a         8154 c         7457 g         7360 t
ORIGIN      
        1 taatttcgtt gggctttttg taggttattt gtacagcaaa atggcgcttg tacatctatt
       61 tcccccaatg ccggatgata aatatcactg gagaatagag aatcatcaat caggtaagag
      121 tctggaattt cacactgtac cctttatact gccctatcac ttcgcgaagt tttaacaggt
      181 cataaacacg aatgcgtcag aaagagacaa cggccacgac ccgcttttca ctcctaccgg
      241 ggagcattac ccgcttcttt ttactgttga tcattgtgtt actggtgacg atgggtgtaa
      301 tggtacaaag cgccgttaac gcctggctga aagataaaag ttaccagatt gtcgacatta
      361 cccacgcaat ccaaaagcgc gtcgatacct ggcgttacgt gacctggcag atctacgaca
      421 acattgccgc gacgacctcc ccctcctccg gcgaaggttt acaagagacg cgcctgaaac
      481 aggatgtcta ctatctggag aaaccacgcc gcaaaacgga agcgttaatc tttggctctc
      541 acgacaactc aacgcttgag atgactcaac ggatgtccac ctatctggac acattgtggg
      601 gcgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg tcaggataac agtctggtgc
      661 tgatctcaac cctgcccctc aaagatctca cctccggatt taaagaatcg accgtcagcg
      721 acattgttga ttcacgtcgt gcagagatgt tgcaacaggc caacgccctc gatgaacgcg
      781 aaagtttttc taacatgcgc cgcctggcct ggcagaacgg tcattacttt accttgcgta
      841 ccacgttcaa ccagccggga catctggcaa cggtcgtggc ttttgatctg ccgattaatg
      901 atttgatccc accgggtatg ccgctggaca gtttccgcct tgagccagac gcgacggcaa
      961 cgggaaacaa tgaaaatgag aaagaaggga cggatagcgt cagtatccac tttaacagta
     1021 cgaagattga aatctcctcg gcactcaact ctaccgatat gcgcctggtc tggcaggttc
     1081 cttacggcac cttactgctg gatacgttgc aaaacattct gctgccactg ctgctgaaca
     1141 tcggtttgct ggcgctggcg ttatttggct ataccacatt ccgccatttc tccagccgca
     1201 gtacagaaag cgtccccagc acggcggtca ataacgaatt gcgcatttta cgggcaatta
     1261 atgaagagat agtctcactg ctgccgctcg gcctgctggt tcacgatcag gaatcaaacc
     1321 gcacagtcat aagtaacaaa attgccgatc atttgctgcc gcatttgaat ctgcaaaaca
     1381 tcaccaccat ggcggaacag catcagggga ttattcaggc gacgatcaat aacgagctgt
     1441 atgagatccg catgttccgc agccaggttg cgccgcgcac acaaattttc attattcgcg
     1501 atcaggatcg cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga
     1561 aaaaccagca ggggcggatg acctttatga aaaacattgg cgatgcgctg aaagaacccg
     1621 cacagtccct ggcggagagc gcggctaaac tcaacgcccc ggaaggcaaa caactggcga
     1681 atcaggctga tgtgctggtg cgactggtcg atgaaataca gttagcgaac atgcttgcgg
     1741 acgatagctg gaaaagtgag acggagctgt tctccgtgca ggatttaatt gatgaagtag
     1801 tgccttcagt gttgcctgcc atcaagcgta aaggtctgca actgctgatt aacaatcatc
     1861 tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt acgacgtatt ttgctgctac
     1921 tgatgcaata tgccgtgacc tcaacgcaat taggaaaaat cacccttgag gttgatcagg
     1981 atgagtcctc cgaagaccgc ctgacgttcc gcattctgga cactggagaa ggcgtaagca
     2041 ttcatgaaat ggataatttg cacttcccgt ttatcaacca gacccaaaac gatcgctatg
     2101 gcaaggcgga cccgctggca ttctggctga gcgatcaact ggcgcgtaaa ctgggcggtc
     2161 atttaaacat caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc
     2221 tcgcagctga cccggaagtt gaagaggaag aagagcgatt actggatgat gtctgcgtaa
     2281 tggtggatgt tacttcggca gaaattcgga atattgtcac tcgccagtta gaaaattggg
     2341 gtgcaacctg tatcacaccc gatgaaagat taattagtca agattatgat atctttttaa
     2401 cggataatcc gtctaatctt actgcctctg gcttgctttt aagcgatgat gagtctggcg
     2461 tacgggaaat tgggcctggt caattgtgcg tcaatttcaa tatgagcaac gctatgcagg
     2521 aagcggtctt acaattaatt gaagtgcaac tggcgcagga agaggtgaca gaatcgcctc
     2581 tgggcggaga tgaaaatgcg caactccatg ccagcggcta ttatgcgctc tttgtagaca
     2641 cagtaccgga tgatgttaag aggctatata ctgaagcagc aaccagcgac tttgctgcgt
     2701 tagcccaaac ggctcatcgt cttaaaggcg tatttgccat gctaaatctg gtacccggca
     2761 agcagttatg tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa
     2821 aatacatcag cgacattgac agttatgtca agagcttgct gtagcaaggt agcctattac
     2881 atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc
     2941 aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca
     3001 ctgatcaaca atctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct
     3061 ggcgataagt acggtgatgg cattacctta atcaagtaca tcaagcgcca tttcccaagc
     3121 ctgtcgatca ttgttctgac catgaacaac aacccggcga ttcttagtgc ggtattggat
     3181 ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatttgcc gaaggctctc
     3241 gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa
     3301 atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga agttctgcgc
     3361 ctgtttgcgg aaggcttcct ggtgaccgag atcgctaaaa agctgaaccg cagtattaaa
     3421 accatcagta gccagaagaa atctgcgatg atgaagctgg gtgtcgagaa cgatattgct
     3481 ctgctgaatt atctctcttc tgtgacgttg agtccggcag ataaagacta atcacctgta
     3541 ggccagataa gacgcgtcag tgtcttatct ggcatttgca cggattgccg gatgcggcgt
     3601 aaacgcctta tccgtcctac gattcccatt atttcaacag attacattaa agtaggccag
     3661 ataagacgcg tcagcgtcgc atctggcatt tgcactgaat gccagatgcg gcataaacac
     3721 cttatccgtc ctacgattcc cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct
     3781 gttttatcac atccagcgtt accggcttcg acaggcagct gtccataccg gactccagac
     3841 accgctgctt ctcttcagcc aacgcattag cagttactcc gattaccggc aacgtcagtc
     3901 ccaactgacg aatgcgttgc gtcaagcggt aaccatccat atttggcata ttgacgtcgc
     3961 taagcacgat atcaatatga ttcttgttaa gtacattaag cgcatcgacg ccatcattcg
     4021 cggttttaca ttgatagccc aacgatccca actgatctgc cagcagacgc cggttaatcg
     4081 gatgatcatc cacgaccaga atcatcatat cgtcattatc gctgaccgct ttgtccgtcg
     4141 acggcagagc gttagcagga tcgtcgctct ccatctcgat caaataaata cgcgccaaca
     4201 atgccggtag ctcatgcgga gcagccacac tatgtaccca ctcccctggc gctttctcca
     4261 gcggaatacc aatatggcga cgacagaagg tcactaccgc tctgccctgc cattttttgt
     4321 tcactacctc gtcagtaatt aacacatctt cgggagtcgg ttcctgccct tcgtatgttg
     4381 taacgacgat gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc gacgcattgc
     4441 ggaccgccag ccagcagcgt ttaccactca acccttccac accttttttc tgcgggtact
     4501 gagcgccgta taacggaata cgcacggtaa actggctgcc cattcccggt tctgaatcta
     4561 ccgagatgtc gccgtccatc atgctgatca gtttttcaca aatcgccaga cccagaccag
     4621 tcccctggaa gttacgctgt acgcccgttc cgacctggaa gaagggatca aacaagcgca
     4681 ccacttcttt cgccggtatc cccacgccgg tatcgcgaac acggatagag agataatcgc
     4741 catcagcgcg aacatgcaaa actatacagc cggtatcggt gaattttatg gcgttactca
     4801 acaggttgga gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc actggcacat
     4861 ccggttcaat aaagcagtac aagcctaact gcttgcgtac caccagcggt aaatagttgg
     4921 cggtgatgtg gttcatcact tcacgcggtg aaaactcacg cggttcgatc ttcaactgtt
     4981 ctgattcaat tttcgagaaa tcgagaatat cgctgataat tttcaacaac aggctggaag
     5041 agttgttcat tgccgtcacc agacgatcga cgcctttcgg taactctttg gtttgcaaca
     5101 gatccaggtt accgataatg ccatacagcg gcgttcgcag ctcatgactg acggtggcca
     5161 ggaacatcga ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact
     5221 cttccatctt cacgcgcgaa gaaacatcca ccagcacaca aatggccacg ttttcattac
     5281 gatagcgcga atggacgaag ctgatttgca gattggtatt gttgctggtc aggacatcaa
     5341 caaaattgac ctgctgccca cagataattt gtgtcagccg ttggcggtcc tcatgcgtaa
     5401 gcatattgag ataagtatgc gccagttcgt tacttaaaat attgacgcca tcagcggtac
     5461 gcaaaatgca gatacccact ggcgcggagg cgacaatctt gcgattaaac tgctcatgtt
     5521 cttccagtcg cagggcgtcg ctttccgccg gaatgaaaat acgccgctcg tacatccgcg
     5581 cgagagtaaa caatgcagct ccggcaagca cattcagcaa aattgcgtta aggatcaaca
     5641 tgcgaatgcg ttccagcacc ttatcaactg gcactgaata aacgatgctt agcgatgagg
     5701 gtggcagatt tttcttcagc accagttccc ggaacccttc cgtatagcca aaccaggagc
     5761 gttcctgcat ccagcgagga tcgcccttaa ttttgctttc tggtccggta agcgaaatca
     5821 gggtatgacc attttcatca agaatggtaa cccccatcgg caacgtacca ggtaagaaaa
     5881 agttctccat ccggatggtt tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat
     5941 aaactggcgt caaggcgtaa aaatacccga cgccagggcg cggaccttcg ctgatccagt
     6001 agaggttact gccactatca tcttgtggtg catttcgata tttattaatg cgttcatgca
     6061 aagcttttag cgcggtatcg cgttccactg gcatatcacg cagaccgaaa ttggccatgc
     6121 agaggttatc gctgccgatt aaaaataccc ggtttagatc gtaagccgca gaaaaattat
     6181 cgcgccagta gcgcataaac cacgccaatg actccagaga acctcgccag gtgttactca
     6241 ttgcggaaca atcagagtca gcaaacagcg gttcaaacgc aggcacatcc gtctgcgttt
     6301 ctcgtccacg cggggaaagc acaccgtttt ctgccgataa gcgattttca gctatgtact
     6361 tcagctcttt catcacatca gaggtgcgtt gaataaagcg ctgagcctga tcggaactca
     6421 gattaaattc ctgacgaatt tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa
     6481 cggatgaaaa agcaatcaac agccagagca ctaacgccaa tgctctgaac atgtagcgcg
     6541 aggctttcag ggttgtacga aaagaagcaa ggtatttcaa aggggcgaag ctccgcctca
     6601 ggtgaccgat ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa tgttctctct
     6661 tctctggaat atgatacacc gccgagaaat catcacctta acctctgata atcgtcatat
     6721 accggacaag actagtggat ttcagcatgc attatatgaa gtggatttat ccacgccgct
     6781 tacgcaatca aatgatcctg atggcaatcc tgatggtcat tgtcccaacg cttactattg
     6841 gttatatcgt agaaacggaa ggacgttcag cagtcttatc tgaaaaagag aaaaaacttt
     6901 ctgccgtggt caacctgctt aatcaggcgc taggcaatcg ctacgatctc tacatcgact
     6961 taccgcgtga ggagcgtatc cgcgcattaa atgcagaact tgcccccatt accgaaaata
     7021 tcactcacgc cttccctggc atcggcgctg gttattacaa caaaacgctg gatgcgataa
     7081 tcacctacgc gccttcagcg ctatatcaga ataatgtggg cgttaccatt gccgcagatc
     7141 accctggtcg cgaagtcatg cgtacaaata cccctttggt ttattcaggc aggcaggtgc
     7201 gcggcgatat tttgaattca atgattccca ttgagcgtaa tggtgaaatc ctcggctata
     7261 tctgggccaa tgaattaacc gaagatattc gccgccaggc ctggaaaatg gatgtgagga
     7321 ttatcattgt gctcaccgcc ggtttgctga taagcctgct gttgattgtc cttttctccc
     7381 gtcgcctgag cgccaatata gatatcatca ccgatggcct ctcgactctg gctcaaaata
     7441 ttcccactcg attaccacaa ttgcccggtg aaatggggca aatcagtcag agtgttaaca
     7501 acctcgccca ggcactgcgt gaaacgcgaa cacttaacga tctgattatt gaaaacgctg
     7561 ccgatggcgt cattgccatt gaccgccagg gtgatgtaac caccatgaac ccagcagcag
     7621 aagtcatcac tggctatcaa cgtcatgaac tggtagggca gccttactcc atgttgttcg
     7681 acaatactca gttctacagt cccgtactgg atacgctgga acatggcacc gaacatgttg
     7741 cactggagat cagttttcca ggccgtgacc gcaccattga actcagtgtc acgaccagtc
     7801 gtattcataa cacgcacggt gaaatgatag gtgctttggt gattttctct gatttaactg
     7861 cccgcaaaga aacccagcgc cgcatggcgc aagcagaacg cctcgccaca ctgggtgagc
     7921 tgatggctgg cgtggcgcat gaagtacgta atccgctaac ggctattcgt ggttatgtac
     7981 agatcttgcg ccaacaaacc cgtgacccaa tacatcagga atatctgtcc gtagtactca
     8041 aagaaatcga ttcaattaac aaagttattc agcaattgct cgaattttca cgcccacgcc
     8101 acagtcaatg gcaacaagtc agcctcaatg cattggttga agaaactctg gtactggtac
     8161 aaaccgccgg cgtacaagcg cgggtcgact tcataagcga actggataat gaattaagcc
     8221 cgattaacgc cgatcgtgaa ctgctcaaac aggtactact gaatatcctg atcaatgccg
     8281 tccaggctat cagtgcacga gggaaaattc gcattcgaac ctggcagtat agcgactcgc
     8341 aacaggccat ttcgatagag gacaacggca gtggcattga tctctcgctg caaaaaaaga
     8401 tcttcgatcc ctttttcact accaaagcct caggaaccgg gcttggtctg gcgttaagtc
     8461 aacgcatcat taatgcccat cagggtgata ttcgcgtcgc cagtttgccg ggctacggcg
     8521 caaccttcac gcttatttta ccgatcaacc cgcagggaaa tcagactgta tgactgctat
     8581 taatcgcatc cttattgtgg atgatgaaga taatgttcgc cgtatgctga gcaccgcttt
     8641 tgcactacaa ggattcgaaa cacattgtgc gaacaacggg cgcacagcat tacacctgtt
     8701 tgccgatatt caccctgacg tggtgttgat ggatatccgc atgccagaga tggacggcat
     8761 caaggcacta aaggagatgc gcagccatga gacccggaca cccgttattc tgatgacggc
     8821 ctatgctgaa gtggaaaccg ccgtcgaagc actacgctgc ggagccttcg actatgttat
     8881 caaaccgttt gatctcgatg agttgaattt aatcgttcag cgcgctttac aactccagtc
     8941 aatgaaaaaa gagatccgtc atctgcacca ggcactgagc accagctggc aatgggggca
     9001 cattctcacc aacagcccgg cgatgatgga catctgcaaa gacaccgcca aaattgccct
     9061 ttctcaggcc agcgtcttga ttagcggtga aagcggcacc gggaaagagt tgattgccag
     9121 agcgattcac tacaattcgc ggcgggcaaa ggggccgttc attaaagtca actgcgcggc
     9181 actgccggaa tcgttgctcg aaagtgaact gtttggtcat gaaaaaggtg catttactgg
     9241 tgcacaaacc ttgcgtcagg gattatttga acgtgccaac gaaggtactc tgctcctcga
     9301 cgaaattggc gaaatgccgc tggtactgca agccaaatta ctacgcattc tgcaggaacg
     9361 ggaatttgaa cggattggcg gccatcagac cataaaagtt gatatccgcg tcattgctgc
     9421 caccaaccgc gacttgcagg caatggtgaa agaaggcacc ttccgtgaag atctctttta
     9481 tcgccttaac gttattcatt taatactgcc gcctctgcgc gatcgccggg aagatatttc
     9541 cctgttagct aatcactttt tgcaaaaatt cagtagtgag aatcagcgcg atattatcga
     9601 catcgatccg atggcaatgt cactgcttac cgcctggtca tggccgggaa atattcgaga
     9661 gctttccaac gttattgaac gcgccgtcgt gatgaattca ggcccgatca ttttttctga
     9721 ggatcttccg ccgcagattc gtcagccagt ctgtaatgct ggtgaagcaa aaacagcccc
     9781 tgtcggtgag cgtaatttaa aagaggaaat taaacgcgtc gaaaaacgca tcattatgga
     9841 agtgctggaa caacaagaag gaaaccgaac ccgcactgct ttaatgctgg gcatcagtcg
     9901 ccgtgcattg atgtataaac tccaggaata cggtatcgat ccggcggatg tataatgcca
     9961 aaacttgcta tgcagaaatt tgcacagtgc gcaattttct gcatagccgc tcattctcct
    10021 tatgaacccc catctaattt atccttttct attcaattag ttaaataaca acattcaata
    10081 atctcattct ggcactcccc ttgctattcc ctgactgtac ccacaacggt gtatgcaaga
    10141 gggataaaaa atgaaaacaa aattgatgac attacaagac gccaccggct tctttcgtga
    10201 cggcatgacc atcatggtgg gcggatttat ggggattggc actccatccc gcctggttga
    10261 agcattactg gaatctggtg ttcgcgacct gacattgata gccaatgata ccgcgtttgt
    10321 tgataccggc atcggtccgc tcatcgtcaa tggtcgagtc cgcaaagtga ttgcttcaca
    10381 tatcggcacc aacccggaaa caggtcggcg catgatatct ggtgagatgg acgtcgttct
    10441 ggtgccgcaa ggtacgctaa tcgagcaaat tcgctgtggc ggagctggac ttggtggttt
    10501 tctcacccca actggtgtcg gcaccgtcgt agaggaaggc aaacagacac tgacactcga
    10561 cggtaaaacc tggctgctcg aacgcccgct gcgcgccgac ctggcgctaa ttcgcgctca
    10621 tcgttgcgac acacttggca acctgaccta tcaccttagc gcccgcaact ttaaccccct
    10681 gatagccctt gcggctgata tcacgctggt agagccggat gaactggtcg aaaccggcga
    10741 gctgcaacct gaccatattg tcacccctgg tgccgttatc gaccacatca tcgtttcaca
    10801 ggagagcaaa taatggatgc gaaacaacgt attgcgcgcc gtgtggcgca agagcttcgt
    10861 gatggtgaca tcgttaactt agggatcggt ttacccacaa tggtcgccaa ttatttaccg
    10921 gagggtattc atatcactct gcaatcggaa aacggcttcc tcggtttagg cccggtcaca
    10981 acagcgcatc cagatctggt gaatgctggc ggacaaccgt gcggcatttt acccggtgca
    11041 gccatgtttg atagcgccat gtcatttgcg ctaatccgtg gcggtcatat tgatgcctgc
    11101 gtgctcggcg gtttgcaagt agacgaagaa gcaaacctcg cgaactgggt agtgcctggg
    11161 aaaatggtgc ccggtatggg tggcgcgatg gatctggtga ccgggtcacg caaagtgatc
    11221 atcgccatgg aacattgcgc caaagatggt tcagcaaaaa ttttgcgccg ctgcaccatg
    11281 ccactcactg cgcaacatgc ggtgcatatg ctggttactg aactggctgt ctttcgtttt
    11341 attgacggca aaatgtggct caccgaaatt gccgacgggt gtgatttagc caccgtgcgt
    11401 gccaaaacag aagctcggtt tgaagtagct gccgatctga atacgcaacg gggtgattta
    11461 tgattggtcg catatcgcgt tttatgacgc gttttgtcag ccggtggctt cccgatccac
    11521 tgatctttgc catgttgctg acattgctaa cattcgtgat cgcgctttgg ttaacaccac
    11581 aaacgccgat cagcatggtg aaaatgtggg gtgacggttt ctggaacttg ctggcgtttg
    11641 gtatgcagat ggcgcttatc atcgttaccg gtcatgccct tgccagctcc gctccggtga
    11701 aaagtttgct gcgtactgcc gcctccgccg caaagacgcc cgtacagggc gtcatgctgg
    11761 ttactttctt cggttcagtc gcttgtgtca tcaactgggg atttggtttg gttgtcggtg
    11821 caatgttcgc ccgtgaagtc gcccggcgag tacccggttc tgattatccg ttgctcattg
    11881 cctgcgccta cattggtttt ctcacctggg gtggcggctt ctctggctca atgcctctgt
    11941 tggctgcaac accgggcaac ccggttgagc atatcgctgg gctgatcccg gtgggcgata
    12001 ctctgttcag tggttttaat attttcatca ctgtggcgtt gattgtggtg atgccattta
    12061 tcacccgcat gatgatgcca aaaccgtctg acgtggtgag tatcgatccg aaactactca
    12121 tggaagaggc tgattttcaa aagcaactac cgaaagatgc tccaccgtcc gagcgactgg
    12181 aagaaagccg cattctgacg ttgatcatcg gcgcactcgg tatcgcttac ctggcgatgt
    12241 acttcagcga acatggcttc aacatcacca tcaataccgt caacctgatg tttatgattg
    12301 cgggtctgct gctacataaa acgccaatgg cttatatgcg tgctatcagc gcggcagcac
    12361 gcagtaccgc cggtattctg gtgcaattcc ccttctacgc ggggatccaa ctgatgatgg
    12421 agcactccgg tctgggcgga ctcattaccg aattcttcat caatgttgcg aacaaagaca
    12481 ccttcccggt catgaccttt tttagttctg cactgattaa cttcgccgtt ccgtctggcg
    12541 gcgggcactg ggttattcag gggccgttcg tgatgcccgc agcccaggcg ctgggcgctg
    12601 atctcggtaa atcggtaatg gcgatcgcct acggcgagca atggatgaac atggcacaac
    12661 cgttctgggc gctgccagca ctggcaatcg ccggactcgg tgtccgcgac atcatgggct
    12721 attgcatcac tgccctgctc ttctccggcg tcattttcgt cattggttta acgctgttct
    12781 gacggcaacc ctacagacag aaggaatata aaatgaaaaa ttgtgtcatc gtcagtgcgg
    12841 tacgtactgc tatcggtagt tttaacggtt cactcgcttc caccagcgcc atcgacctgg
    12901 gggcgacagt aattaaagcc gccattgaac gtgcaaaaat cgattcacta cacgttgatg
    12961 aagtgattat gggtaacgtg ttgcaagccg gactggggca aaatccggca cgtcaggcgc
    13021 tgctaaaaag cggactagct gaaacggtgt gcggattcac ggtcaacaaa gtgtgcggtt
    13081 caggtctgaa aagcgtggcg cttgctgcac aggcgattca ggcaggtcag gcacagagca
    13141 ttgtggcggg gggtatggaa aatatgagtt tagcgcccta cttactcgat gcaaaagcac
    13201 gctctggtta tcgtcttgga gacggacagg tttatgacgt aatcctgcgc gatggcctga
    13261 tgtgcgccac ccatggttat catatgggga ttaccgccga aaacgtggct aaagagtacg
    13321 gaattacccg tgaaatgcag gatgaactgg cgctacattc acagcgtaaa gcggcagccg
    13381 caattgagtc cggtgctttt acagccgaaa tcgtcccggt aaatgttgtc acccggaaga
    13441 aaaccttcgt cttcagtcaa gacgaattcc cgaaagcgga ttctacggct gaagcgttag
    13501 gcgcattgcg cccggccttc gataaagcag gaacagtcac cgccgggaac gcgtcaggta
    13561 ttaacgacgg tgctgccgct ctggtgatta tggaagaatc tgcggcgctg gcagcaggcc
    13621 tgaatcccct ggcacgcatt aaaagttatg ccagcggtgg cgtgcccccc gcattgatgg
    13681 gtatggggcc agtacctgct acgcaaaaag cgttacaact ggcggggctg caactggcgg
    13741 atattgatct cattgaggct aatgaagcat ttgctgcaca gttccttgcc gttgggaaaa
    13801 ccctgggctt tgatcctgag aaagtgaatg tcaacggcgg ggccatcgcg ctcggacatc
    13861 ctatcggtgc cagtggtgct cgtattctgg tcacactatt acatgcaatg caggcacgcg
    13921 ataaaacgct ggggctggca acactatgca ttggtggcgg ccagggaatt gcgatggtga
    13981 ttgagcggtt gaattaacca ataaaaacgc ccgatagcga aagttatcgg gcgttttctt
    14041 gaacatcgac ggcgaaggtt accccattaa tcaccagtca aaacttttca ccagcgtcag
    14101 ctcgccagca ttacgcatcg gtacaataaa agtttcctgt ttctcattga ccgacccttc
    14161 atcggtgatc agcgtcagtt gggccgtagt taattccgtt tcactgcgcc cgccgaagta
    14221 gttgatatac acctgataac ggccgtgaac tggcgctggc atggcgaaaa tctcgggtcc
    14281 gtaccctgtc gtgacatcca tatccagtgc gccactgttt ttcagcactg tgttaccgta
    14341 ccaggcatgt tcaccgtcag gcgtaacgac atgaagatcg aggtcggtat tgtccgtatc
    14401 ccaggagaga accagccgta aacgcgcgcg aatcgcccca gcacccggcg ttgagtaaaa
    14461 ctgcattttt tgtcggcttt gcccatctgg gctgatgacc tgcacgctgt tgctgccttc
    14521 agtgaaaata taagggcgtg caaaagaacc atctgattca attctctgcg gcatactggc
    14581 accgttgacc accagccggc cctgctgaac tttacccgcc gcagcataat tctttatttt
    14641 gccgcggatc tgcgctgaga tattttgatc atctgccatg ttgacggacg acgccgggta
    14701 attgatcgtt tgtgaaaaac tggcatcttc gccctctgcc ggatgccagc cagagagcgg
    14761 tgcatcaatc tcgacttcct gcacaccttc actgtgagcg acaggcgaaa gcgccaccag
    14821 taacaacgga agaaaaatct ttcgcatagc attagtccat taataattgt cgggtcagcg
    14881 tttcgatata gttctcatca agcccggtgg gataaccatc aaaggccagg tgcagatact
    14941 catgggttaa atccagccga tcctgaagcg taaagaaatt acgaataaac agccgtttct
    15001 gctgccgatc ggtatagggg aaacctgaga ctaaacgaca gaccgcaaac acgtctggtt
    15061 cgttgtatcc cgtctcacct tgtaatatac gccgccactg cggcattttt ttcgccagcc
    15121 aggctttcgc tttgggtaat aactggcagg ttgagcgtgg cgctccccag cggctaaggc
    15181 tgttgtcggg ataagcaaac gcgagtatct gatcataacg ctctccctgc cccgcctggg
    15241 cagtagcgtg ccgccaggaa agcgtccctt cggtagcgcg actgccatga taatgaaccg
    15301 gatcgcccgc atagatgaga tcctgcgtcc aggcggtcat cgttcgcgcc cccgtggtag
    15361 ctggcgaagc agaaacacgc tgcgtagcgc tgctgtccgg gatggtcagg caatcgcctt
    15421 cccgatcggc gttttgctgt aaaaacgtgc gaatcgccac ggtcatggct ttcgctgcct
    15481 caggaggcgt acttttcgcc tcgcgatcca gaactctggc aacgtactct tcacgatcga
    15541 gatgcgactg taatttcagt ttgcccttta ccgtcagtaa cgttgtctca ccgtggctga
    15601 caaatgtcat gtggttacca ttggcaaacg tcacacgata gcgcccattg agcacgccta
    15661 gtttgactgc cgtcgtactc ttttcctcag taactttctt cagcggataa cgagcaaaca
    15721 gatccaccag cacacattgc ccactggcaa cttgcgtcgg cactggcaaa acccgattaa
    15781 gcgccgtcgc ataacgggtc agtaccgttt tgctggtgcc actgccagta acccacagcg
    15841 gtgtaccatc ggttaaccag ccagcaaaac caccctgacg gatttcctgt ttatcatcgg
    15901 caaaccagct ccaggttttc acccgtaccc gactaccgag catggatgcg acaccgattt
    15961 tcgcctcgtc cagcaccaca tccagtaaga cttcctgagc tttgttttgc gcaggtaacg
    16021 tcgctaatga atcaagcagc gatttaaccg ttaccgaggt ttctggcttc agtgtagtaa
    16081 gagacgctaa ccacgcaggt gcctgacgct gttgccagta ttgcccccac atatctgcgc
    16141 caatatgcaa ccgctgcggc gcaaaataga gtccacagga acgtaccagc gcggaatcgc
    16201 gcgttatgct ctccccagcc tgacaacaat aaacctcttc cggggagttg ccgcgacact
    16261 ggtatggctg ctccggttga tgggtatcct ccagccaggc gtaaacatac agtttccaca
    16321 aactccctaa tggcgtggtg agcgtgtcag gtaataccga gactttggtc acgcctgatg
    16381 acgacagctg atacagttga tcgtgctgcg caccacgcag cgccagctgt aacggcgtct
    16441 cctccgcaag cgttggcaga gttaccaacg ccaatagcca gacgattctg taccagttca
    16501 cttatttcac ctgcatcccg atccattcgc tccctggcgc aacactttgc tgtgcaggtg
    16561 cataggaacg cacataacgg gcaggcggca gaacgaattg ccctttttgc gagaaacgca
    16621 gcaaatggcg gaaggtgacc gttcccgtca gttcttttac cggcaccata taggccagtt
    16681 cacccatttc attgcgcgct ttttccagca gttgtccttg ctgttttccg gcgttcggtt
    16741 tattgaccga aatgccccag gttgtacgct caacgtcagc ccccggcggc agcggtactt
    16801 ccacctgacc atagcgcaga acggcatcct gctcgctggt aagcgtgatt tcatcgagat
    16861 acagcgcatc gctgtcaatc tcattgctgg tgacaggctg taaggtaaag ctcatctctt
    16921 cttcaccggg gataagccga tacaactggc gttcaacggt caccggaata ttactttgtt
    16981 gagccgtttt tgccggttca cgccagcgga cctgcacgtt ttgcggcggt aattcgtcac
    17041 caaaagagag aatgtccggc acgccctgac caacccaacg ccagtattcg cctcctccgg
    17101 ttaacttatg tttggcccat gcgcccgcag gcgcaggtaa cacaaccgga ggcatcgtcg
    17161 ccatatattt cgccagccag ttcatggcca acgcgcgctc aatggtggat tgctcagcgg
    17221 ttaaaccact taaaatagcg gccgcctggg tagcgtcgcc tccactattg agcagcacaa
    17281 ccgtgtgcgc cagcggttgg ttgctgtttg ctgctttatc ccacgcatat tgctgaacct
    17341 gtggcatcac agtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    17401 ccaccgcatc tgccagtgga gattcaggtg tatcgaggat catgctgtca ttgatatcgc
    17461 tggtgtcttc ctcagagaaa tcttcagttt tagttccgcg ccgggcgata gcttcatcca
    17521 accctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    17581 cccgatgcaa taacggcatg ttgtccgcct gctcggcata actgtcgagc atatgttgcc
    17641 agtattccgg ttgctgcgtt acgccgagcg cctggctggc ctgccagtcg gcgtaccatg
    17701 cccatgccgt gaggaaggcg tcgccattgc catcttcacc ccaccaggta aagcgcgctc
    17761 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    17821 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    17881 ggctaccggt gttgatcacg ccaccccacg gttcatcgac taacgcatca agattgttac
    17941 ggaaaatctc ctgcggcgtt tcgctacttt gtagccggat attgctcgcc tgctcgggca
    18001 acatcagcgc attatcgcca ccgccgagca tgacattttt ctgttgttca acgggccacc
    18061 tgttatccac aaaagacagt tttgtgctaa tgctgtcctg tacttgccca ttttgttgca
    18121 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    18181 ctttgtgcag cgtcagcgtc tggcgcatct cagcacctgc aaatttagtc accagcgcca
    18241 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcacccatgc
    18301 gatataccgt tggcatactc cacttcatgt agagattttt ttccgaacgc aaataagcac
    18361 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatgcgc cagcgggtta
    18421 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    18481 gcatccaggc cgcggtatcc acctcttcac gccgtggacg ttccagcatt tttactcgcc
    18541 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tgatcgtagc
    18601 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    18661 tgccgatatt cggcgcgatt tccggttgca gcgcgtagat catttcatcg accacgccta
    18721 ccgttaactg cgcagaaaca ggtttacctt tcagcgacga ggttaattcg acattgacca
    18781 gttcaccagg ctggtaatgg gttttgtccg ttttcacccg gatatccagc tggggaacgg
    18841 caactttaat cccggcgttc tgaaaactgt actgaccgtt acgggtatac agcaccgaaa
    18901 aagtgatgtt aggcgcaaag gaattgctca ccggtacgcg ggcttcatac tgggtatcgt
    18961 ttaaacgttg tagcgttagc cagtttgccg gatgcgatag cagcgactgc tgttccacgc
    19021 gatcgcgttc cagcgtcaat aatgcttcgt caattggctc cgggaaggta atcagcatct
    19081 tcgcggtttc gccaggctga tacagtgttt tatctgccac gatatctacc gtaccactat
    19141 gcgacgtgct gtccttaccg ctgactgcat ggcttaaccc ggcgagaatt aagccgtctt
    19201 tatcgcgtaa tgtcagattg tagttgccag gtttagcgaa attgacggta aaggatttgc
    19261 cgcctgactg tagctctccg ctgtgactcg tgcggtcttc gagacgcaac cattcatacg
    19321 taacaggaac ctgttttgaa gattccagcg cggcataacg gaacacaacc gactcgccgc
    19381 tattactgta ttgtgcggcg gtacttaaag agtaatgcgc caggccacgt tcaatgagga
    19441 tctctttggt ggtggtaact cgatacgccg cgccgtcact ggcggagacg gttaacaaat
    19501 aacggctcgg tttatcggcg gcggggagat tcaacgccac atgaccatta tcgtcggaaa
    19561 ccgtttcact gccttccatc gacaccggga aacgtccggc ataacgcaaa tcgttaccga
    19621 ccattgataa ttgctgagcg cgcaaactta actgcacgcg ggcatctttt accggctcgc
    19681 catccgggta aagcagttgc agtttgccgc tgaccgcttc gccagttttg aactcttttt
    19741 tgtcgagagc taaaccaatc tcaaaatggg gtttgatgta gtttgccacg cgaaaactgc
    19801 tgctatagac ctgtttgcgg taagcaagac gtaactcata acctccggcg acggcatttt
    19861 ctggcaggcg gaaactcccc tgcccgccat tacgcgcatc cagcgtgaca ttgacggttt
    19921 gcaacagact gccgttggcg tccagcaccg aaagcttcgc cggtgcgctg acgatggggg
    19981 atgaatgcaa cggatcgtgg aactcgcgac ccatcacttt aacatcgaca cgatcgcctg
    20041 cgcgatatag cgggcgatcg gtaaagatat acaagcgggt gttgtagatt tcgctttcgt
    20101 agaaaaagtt ctcggagaca aaaacgccgc cttcagcatc cttacccaga atgtatgaac
    20161 gttctggcga tatatgttgt aactgcaagg taccgctgcc atcggtcaca ccgcgggtca
    20221 tcacgccaag accgtcagtc cacaagatct ctgagccggg cttcgcttca ccctgttttt
    20281 tccccgcggt ccacaccaga agctcgttac ctgacacttt gctaagcgcc acggtatcgg
    20341 aaacaaacac caccgtcgtc gcccgatacc cacccaccat cgcttcgacg agatacagtc
    20401 ccggctcttg tttgccgaga ggaatataga tgttgcccgg ctgcggcgag atgaaattgc
    20461 tggatgcgcc ttccagtttt accccttgct gcggctcgac cggttttgcc tgccatagtg
    20521 gatagcggaa ctgttccaca agtggatatt ttttcagcgg tgaaaactgg ctgttctgta
    20581 cataacggga aggtttaaca atggcattac cgagctgtaa ttcgggtaac gcctgagtca
    20641 cattctgccg tgactgagaa gagaaagtac gctgcatcac gcggcgagat ttgccgtacc
    20701 agttatccca cagccaggtc agcgtattgt tcagtccgtc gcccagatat tgcggttgca
    20761 ccacaatgcg atgcaggttt ttctgctggc gcaaaaatgc catcgggtca ggaatacgat
    20821 acaggcgaac gtccacgccg ccgtactctt ccatctgata gcgccgataa tcacgccccg
    20881 gcgcttccag tcgcactttc gcctcttcac tgctgctaaa actgctgtca gcgagcaaaa
    20941 agaatgttcc cccggcgggc ggcacatagt tactggaagg aagcgaatcc tcagcattag
    21001 caagccctgt tcccacaaga gacagggaca agcacgctgc tatcgcgcca gaaaatttaa
    21061 acgataaatg ccaatgaaat tgggattgga atcgttgggt atccatcggg tgtccttcca
    21121 tgtcataagt tgttgcagac tgactgcgcg cattccgttg tcagttttcg tggcgcttcc
    21181 ggtgtggtag atgatgtaac gccccatcca gaccattaag tgctgggcat cgccctgatc
    21241 gaaaaaaatc atatcgccag gcagtgcctg gtttatgtcc tggccaataa actggctgtt
    21301 gtactgaatc aaattaatcg cggtcacata ggggccggtt ttcccattcc cctgattcca
    21361 gttttgcgcc agttgacgct gttcaggtgt tagcgtcatc tctggcggca aatactggtt
    21421 agataaaccg ttacttttta gccatttact gtcgtgaact ttcagcgcct cgttcgccgc
    21481 aaatcgcacc aggcccgcac aatcctgctg ataccagcgt gggcttggcc cctggcggag
    21541 ctgttcctgt gcaatgcgca caaaccacgc gcgaaacagc ccggattgtt cgacatttag
    21601 catttcgcta tgggcaacaa cacaatacag ccagcaaatc agcgccagca gcccgtgtct
    21661 catagcggtt gccaggttat gggtagccac tgccaggcga caccgggttc catctgggcc
    21721 agcttcatga cataacgcgg ttgttgagat aaagcgtcca gcttcggcat taataaagtt
    21781 tgtgcggcgt tataaaaaac cggttcgaga ttcttcggca gactggtcag cgtttcgtta
    21841 cgcagcagtt tcgccattcc ttgtggattg atatagagcg gaacgatgcc atcagtgggt
    21901 attacatcca ccattgccgg gcgggtttta ttcagtgttt gcagggcgtt attaaccagc
    21961 gtgtcatcga gggagaaaag cagcgttttg ttttgcatcg ccagcgacac gcggaaaaaa
    22021 taatccgaca ttaattgatc gggttgcgcc gcctgcgctt tcggatactg accgtatcgg
    22081 gaactcactt cgcgacgcca gatttgcgct tcgccctgct gagtctggct taccggcaaa
    22141 acaccttctg gcgctttgct ttcgtgcgca ccaatatttt gcgtaaacag tttccctggc
    22201 aattgcgcct gttcggcagt gccatcaaac tgaccaacaa acagtggggt ttgcaatttt
    22261 gagtcttcat accagcacag ccccgcgcca ccgtctaacg ccccattcag cttgtcgttt
    22321 tcctggctga tgtgcgaaag catctcttcg gcaataccgt gtgaatacgg caccgccaca
    22381 cagaagctgg ctccggcagg catactgttc cataccggcg taaaatcgaa actggcatct
    22441 acgctggcgg attcatcgtt taacgccaga aaactgtgcc agccgtcgtt acccatttcg
    22501 aagcgtacgc cagcaaaaga aggcattaat cgctggtagc caaaccccag caacctggcg
    22561 ctgactacga tgcgctggcg tactggcgtt ttttcggcag tacgcgcttc caggccaaag
    22621 cgggtttccc agcgtttttt accactcaac aaatcacttg cgatcgccgt ggcttcggta
    22681 tcctgttggt catctttaaa cagcatatcc gaactggaaa aagccaatat tttgtcctga
    22741 taagtcgcaa acatcaggac gttattgccg ttatagcgca actgataaac aggaatagtt
    22801 tcactattta tcttgatgct actgatttcc gttttactta actggctgtc gctggtagcg
    22861 gcaaacagca atggctccag cagcttgctt aaaccacttc gctggatcag caccatataa
    22921 tgagaaagat ggccttgttt atcgtgccac agtgccgcct gcgcgggctg gtctaagagt
    22981 gacgaaaaga gcttatcttt cagcgtgaga tcgtgttcat agacaatgcg gcgaatgctg
    23041 ccttcaatgc ccagacgatc ggcatgattc tgataataga aaacgaaatc ttcgctcaga
    23101 acatcgtgga gaaacggaat ggtaaggaga tctttgggaa gctggctcag agagtcgctg
    23161 tcgagaaaga ggtccggctc attgagatcg atttgcagat tgttgtgcac caccagtggc
    23221 gacaacgttt tttctggccc actgccagag tattgtaatg cccatacgcc agcggatagc
    23281 agcgctattg ccccaaaacc aacaaggcca taaaaccgcc agcctttcgc ctttttttca
    23341 ccactcattg ccacattcct tgtgtatagc cagccatttt ttacgggcac agccaaactt
    23401 taccgcgccc taatacgaca aaagcccaga ctttacagcc tggacttttc aattcaaaaa
    23461 agggagatgg ctcccttttg gcatgaagcg atgaaattat tcttcttctg gctcgtcgtc
    23521 aacgtccact tccggagcga tttcatcgtc cccttccgcg gcactgccgt cgatggtatc
    23581 cagatcttcc tcgtcaaccg gttcagcaac acgttgcaga cccaccacgt tttcatcttc
    23641 cgaagtacgg ataaggatta cgccctgggt gttacggcct acgatgctga tttccgacac
    23701 gcgagtacgt accagcgtac cggcgtcggt gatcatcatg atctggtcgc aatcatctac
    23761 ctgcaccgcg ccaaccacta aaccgttacg ttcggtaacc ttgatggaga taaccccttt
    23821 cgtcgcacgc gacttggttg ggtattccgc cactgcggta cgtttaccat aaccgttctg
    23881 cgttgcggtg aggattgcgc catcgccacg aggcacgatc agagagacga ctttatcgcc
    23941 ttcgcctaaa cgaataccac gaacaccggt ggtgttgcag cccatcgcac ggacagaaga
    24001 ctctttaaag cgcaccactt taccttcagc ggagaacagc attacttcgt cttcgccgct
    24061 ggtcaggtca acgccgatca gctcatcgcc gtcaaccagt ttgatcgcca ctttaccggc
    24121 ggtacgcaga cggttgaact cggtgaggac ggttttcttc acagtaccgt tagcggtcgc
    24181 catgaagact ttcacgcctt cttcaaactc ggtcaccggc aggatcgcgg tgatacgttc
    24241 gtcctgttcc agcggcagca ggttgacgat cggacgacca cgtgcgccac gagtagcttc
    24301 tggcaactgg tagaccttca tcgaatagac gcggccacgg ctggagaagc acagaatatg
    24361 gtcgtgggtg ttcgccacca gcagtcggtc aataaagtct tcttctttaa tacgtgcggc
    24421 agatttacct ttcccgccac gacgctgcgc ttcgtattca gaaagcggct gatacttcac
    24481 gtaaccctgg tgagaaagcg tgaccaccac atcttcctgt gtgatcagat cttccaggtt
    24541 gatgtcggcg ctgttagcgg tgatttcggt acgacgtttg tcaccgaact gttcacgaac
    24601 cagctccagc tcttcacgga tcacttccat cagacgatcg gcgctaccca gaatacgcaa
    24661 cagctccgca atctgatcca gcagctcttt gtattcgtcg agcagttttt cgtgctcaag
    24721 gccggtcagt ttctgcaaac gcagatccag aatcgcctga gcttgctgtt cggtcaggta
    24781 gtacagacca tcacgcacgc cgaactctgg ctccagccat tccggacgcg cagcatcatc
    24841 gcccgcacgt tccagcatcg cagcaacgtt gcccagctgc cacggattag caaccagcgc
    24901 agttttcgct tctgcaggcg tcggcgcatg acggatcagt tcgatgatcg ggtcgatgtt
    24961 cgccagcgcc acggctaatg cttcaagaat atgagcacga tcgcgagctt tacgcagttc
    25021 gaaaatggta cgacgggtca ccacttcacg gcggtgacga acaaacgccg cgatgatgtc
    25081 tttcaggttc atgatcttcg gctgaccatg gtgcaatgcc accatgttaa taccgaaaga
    25141 aacctgcaac tgggtctggg agtagaggtt gttgagcacc acttcaccga ccgcatcgcg
    25201 tttgacttca atcacgatgc gcataccgtc tttgtcagac tcgtcacgca gcgcgctgat
    25261 gccttccacg cgtttttctt ttaccagttc cgcaattttt tcgatcaggc gcgctttgtt
    25321 cacctgatac ggaatttcgt ggacgataat ggtttcacgt ccggttttgg cgtcaacttc
    25381 tacttcggca cgggcacgga tatatacctt gccgcgaccg gtacggtaag cttcttcaat
    25441 accgcgacga ccgttaatga ttgccgccgt cgggaagtcc ggccccggga tgtgttccat
    25501 cagcccttca atgctgatgt cttcatcatc gatatacgcc agacaaccgt tgatgacttc
    25561 cgtcaggttg tgcggcggaa tgttggttgc catacctacg gcgataccgg aagaaccgtt
    25621 caccagcagg ttaggaattt tggttggcat gacgtccgga attttttccg tgccgtcata
    25681 gttatcaacg aaatcgaccg tctctttttc gagatcggcc atcagttcat gggcaatttt
    25741 cgccagacgg atttccgtat aacgcattgc cgccgcagag tcgccgtcga tggaaccgaa
    25801 gttaccctga ccgtctacca gcatgtaacg cagcgagaat ggctgcgcca tacggacgat
    25861 cgtgttataa accgccaagt caccatgggg atggtattta ccgattacgt caccaacgac
    25921 acgggcagat tttttatagg ctttgttcca gtcattgcct agtacgttca tggcgtaaag
    25981 tacgcgacgg tgtaccggct tcaggccatc tcggacatct ggcagcgcac ggccaacaat
    26041 gaccgacatc gcataatcca gataggagct cttcagctct tcctcaatgt tgaccggtgt
    26101 aatttctctc gcaaggtcgc tcatctaacc gctatccctc tactgtatcc cggattcaaa
    26161 ggtcgcaaat tataacacag ccgcgcagtt tgaggtaaac ctatacgctt tattcacatc
    26221 caatgcctga tatactcgtt tgtcttgcca attacggagt agaagtgcca atgaatgccg
    26281 aaaaatcgcc ggtaaaccac aacgtagacc acgaagagat cgctaaattt gaagccgtcg
    26341 cctcccgctg gtgggatctt gaaggtgagt tcaaaccgct gcaccgcatt aacccgctgc
    26401 gtctgggcta tattgccgag cgtgctggcg gtttatttgg caaaaaggtg ctcgatgtcg
    26461 gttgtggcgg cggcattctg gccgagagta tggcgcggga gggcgcgacg gtgaccggtc
    26521 tggatatggg ctttgagcca ttgcaggtgg caaaactgca cgcactggaa agcggcattc
    26581 aggtggatta cgtgcaggaa accgtggaag agcacgcggc aaaacatgcc ggacagtatg
    26641 acgtggtgac ctgtatggag atgctggagc acgtccccga tcctcagtca gtggtcagag
    26701 cctgtgcgca actggtgaaa ccgggcggcg atgtcttttt ctcgacgctt aaccgcaacg
    26761 gcaagtcatg gctgatggcg gtggttggtg ctgaatatat tttgcgcatg gtgcctaaag
    26821 gtacacatga tgtgaagaag tttattaaac cggcagaatt gctgggctgg gtggatcaga
    26881 ccagtcttaa agaacggcat atgaccgggc tgcattacaa ccctatcact aacaccttta
    26941 agctcggccc cggcgtggat gtgaactata tgctgcatac gcagaataag tgaggttgtt
    27001 gtttggattc atccggcaac cgcgccaagc ctgatgcgac gcttgccgcg tcttatcagg
    27061 cctacaaatg ctccccgtag gccagataag gcgtttacgc cgcatccggc aacagtgccg
    27121 gtcagacagc gattaccatt tcactgtcat cgacaaaaac cctgccatct gggcaaaatc
    27181 atcactccct ttctgccacg ccacgctgcc gcgcagcgaa actcgctgac tgatattgcc
    27241 cgttattccc acttttattt caccccgttg cttcaccgca tcgtcactga tagtgctgcc
    27301 atcctcttca atttccgtcg catggggatc gtgataataa ttcagatcta acgttggcgt
    27361 gacaccaaca gcagtacgcc attcgctgtg taaacccaac cgcatctgaa tatcatcacc
    27421 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
    27481 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataacc
    27541 ggcttccagc gaggcgataa tccccgacga gtggtaatga tccactccat cgtcttgttc
    27601 ggaaacatca ttgttaaacc acgcatattg cagccagcta tccaaccagg ccccctgctt
    27661 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgc ggttctggtt
    27721 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tggttatcgc tgtagccacc
    27781 aacaatccca agcatccact cgccatcatc accccagtgc ccgctaaaca ggttgccgct
    27841 aagctgcacc gtagaagtgt cttcatgctg agccaactgc cctgccgccg tgtaatgata
    27901 acgcccgccg ataacgcgta aattcagcgt ctgaccatcg ccacctgcgt gatcgcgtcg
    27961 ctccatcaca aatgcctgat ttgccgcacg taggttatta aaatatccac caactttggc
    28021 attcaacacc ggctggtaag caggcgtagg ttcagggtcg ggggttgggt cgggatccgg
    28081 cgtgggatca ggatctggag tcgggtctgg atcaggcggc ggcgtcggat tgacttcttg
    28141 tgatcgcaga taccagtcgt tgttatcttc caccagcgtg tagtcatacg cccccatatt
    28201 gacgtagccg cttcctgcca gactgaactg cgcattgttt tgaaactgcg tgggatcagc
    28261 tgcgaaatca accactttaa tacctgtaga tgtcggctca ccaatccctg taatgggatt
    28321 aatcacaacc gtggtattgc cagcagtatt accgttcaac accaactggt cgcttgccga
    28381 gtcatcgccg tttaattcgc tgtcgagcag tagcgtaccg ccgccggtat aatcaccatt
    28441 taccgtcagc gtgtcgccag cgatgccatt ttgcagactg acagcaccgg acgtattggt
    28501 cagattgcca ttaaccgtca cgtcggaaag ataagtcagg ctgtcagttt caaggttggt
    28561 attaagctca cctgtcaacg tcacgccatc ggtggcattc accagcgtac cgtcgccgct
    28621 aaacagcgag gcatccagcg cctggttcgt atcctgctgc gtcaatcgca acacagtacc
    28681 atcggttatg tcgatatcgc cgccagaggt ggcatcgatt gagcgaatat cctgattttg
    28741 cccggtttca aaaacaccgt cttcccccac ccacagtgaa gaaacataag gaataatatt
    28801 ctccgcttca ccttttagcg ttccgtcttc cacacgtacc gcagacgtcg cagtgccgct
    28861 ggcggtcaac tccagcgtcc cgccgccact tttagtcaac gtgcttacgc tatccagttg
    28921 ttgcccggta ctatccgcca tcaacccgcc ccattgcgta tctacgccat cctcaacgtg
    28981 tatttcgcca tcagcgcgca tttcaatatt cctgccctga cctgttgtag ccgagcgagt
    29041 gtcaacatca gtcgtcacat ccatttcact gtgttgttgg ggatctgtga agatcactga
    29101 acggttgtaa cttgtgtccc ccaattgcga attatcgctg acttcaagcg taccggaggc
    29161 gatttgcgta ttaccgagat aaccgttgtt attcgccagc gttacgcgct gatcccccgc
    29221 attaatgacg acataaacgc cattctggct ggcatcactg ccgccaatat gtaaatcatc
    29281 gccactgctg atatcaacga catcattact gccaatcact gtccccccgc tgccgctgat
    29341 ttgcagaccg tcattgtatg agctatcgcc atcccaggtg gtgaaatcag agagatccaa
    29401 tactccgcca tttagcacga tcgattgcgg atcgctctgc attgcccgta aatcggcctg
    29461 attccccgcc agcgacaata cggcgttatc ttccaccaca atatcgccgg ttaatgccat
    29521 cgactgcgcc cctgtcagga gatagctgcc atcttgcgct accgtcaact gtccttcacc
    29581 ctgaatcgag ccggaaaaac cgccctggtt tacggtaaca ttgccgcctg cgtcgatatt
    29641 caaaatgcca ttagcaaaac cggttaatga gtgcacaaac gtttgttgtg tattgccaac
    29701 attcagttct gcctgatact cagaatgaac agtgctgccg accatcagac caaagcaatc
    29761 ctgcggatcg ctctgacagt gcgtgtcgcc gacattcatt aactcatcgc tgcgccccaa
    29821 cgtcacctcg ccattctgga tactcatctc gccggtaaac gcattattgt cggcattgag
    29881 gactaactct cccgctccct ctttcacgat gactcccgtt ccggcaagag aatcaatcgc
    29941 gccatcgttg ctggtattgc ctattaccag cgttttatcg gcggcaatgt taaattcagc
    30001 cacactatga ccgatataca taaagccccc cgccgctgcc gtcgccgaat ggtcatatcc
    30061 cgaggcgctg ttattttcat caatcatcac accattgttt tggtcataat tatcatcaac
    30121 agaaatatca ataagatacg gggatgtggt gctgctggta taaattgctc cgccataacc
    30181 ttctgcatag ttattggtaa aggaggtatt attaattatc gttttt
//
LOCUS       DACADA010000044        29788 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.044, whole genome shotgun sequence.
ACCESSION   DACADA010000044 DACADA010000000
VERSION     DACADA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.044"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..144
                     /locus_tag="ISB17_001953"
     CDS             <1..144
                     /locus_tag="ISB17_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1979887.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ISB17_001954"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ISB17_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP1979888.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ISB17_001955"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ISB17_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP1979889.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ISB17_001956"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ISB17_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP1979890.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ISB17_001957"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ISB17_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP1979891.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ISB17_001958"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ISB17_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP1979892.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ISB17_001959"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ISB17_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP1979893.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ISB17_001960"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ISB17_001960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP1979894.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ISB17_001961"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ISB17_001961"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP1979895.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ISB17_001962"
     CDS             complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ISB17_001962"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAP1979896.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ISB17_001963"
     CDS             complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ISB17_001963"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAP1979897.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ISB17_001964"
     CDS             complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ISB17_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAP1979898.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ISB17_001965"
     CDS             complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ISB17_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAP1979899.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ISB17_001966"
     CDS             complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ISB17_001966"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAP1979900.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ISB17_001967"
     CDS             complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ISB17_001967"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAP1979901.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18154..18254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ISB17_001968"
     CDS             complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ISB17_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAP1979902.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18877..19650
                     /gene="nudC"
                     /locus_tag="ISB17_001969"
     CDS             18877..19650
                     /gene="nudC"
                     /locus_tag="ISB17_001969"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAP1979903.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19690..20754
                     /gene="hemE"
                     /locus_tag="ISB17_001970"
     CDS             19690..20754
                     /gene="hemE"
                     /locus_tag="ISB17_001970"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAP1979904.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20764..21435
                     /gene="nfi"
                     /locus_tag="ISB17_001971"
     CDS             20764..21435
                     /gene="nfi"
                     /locus_tag="ISB17_001971"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAP1979905.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21478..22068
                     /locus_tag="ISB17_001972"
     CDS             21478..22068
                     /locus_tag="ISB17_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAP1979906.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22255..22527
                     /gene="hupA"
                     /locus_tag="ISB17_001973"
     CDS             22255..22527
                     /gene="hupA"
                     /locus_tag="ISB17_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAP1979907.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22540..23235
                     /locus_tag="ISB17_001974"
     CDS             22540..23235
                     /locus_tag="ISB17_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAP1979908.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ISB17_001975"
     CDS             complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ISB17_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAP1979909.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            23894..25270
                     /gene="zraS"
                     /locus_tag="ISB17_001976"
     CDS             23894..25270
                     /gene="zraS"
                     /locus_tag="ISB17_001976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAP1979910.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25267..26592
                     /gene="zraR"
                     /locus_tag="ISB17_001977"
     CDS             25267..26592
                     /gene="zraR"
                     /locus_tag="ISB17_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAP1979911.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ISB17_001978"
     CDS             complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ISB17_001978"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP1979912.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ISB17_001979"
     CDS             complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ISB17_001979"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP1979913.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6971 a         7731 c         8216 g         6870 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcaactg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtacgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgtattactg cgcgccacat tcgccagctg gaaaaagacg
     4981 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     5281 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actctggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     6481 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     6661 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagataaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     8701 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgtctg ctggacctgg ctgcgccgga tatcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggccgttca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caataaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gtaaagtggg tgcaacttac tcccgcgatc tgctgggtat taccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt ttttatgggt gttattcaga gggcaaagtc
    12541 ttcacgggaa atattaagaa aggcagggcg aaacgaggaa gaagcttttc gccctggaca
    12601 acattcctgc tgacggcact accataaaaa aacacaacgc tttcaacaac cttgcgtttt
    12661 tcatcttccg tagcggctcg gaataaattt acgttcttac attctgtctc atggcctttg
    12721 cgatgggcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac cctgagcggt
    12781 taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact gttccagctc
    12841 ggggtggttg tcggcatagc cacctggctg cgttttcgaa aaagcgctga cgttattaat
    12901 tgccagcgga atcacgcgat cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc
    12961 aatctccggt gcaagcagcc ggaaagcgca gatggcttgc actaactggc gttcatccat
    13021 aatcgacgca ggctcaatgc cgccagtaca tggacgcagg cgtgggaagg agacagagta
    13081 gcggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca tatagcagtc
    13141 aactcgccag ctgtcggaaa ggccgattag cgcgccgagg cctatcttat caatccccgc
    13201 acgccctagc cgatccggcg tttccagccg ccagaagaag tcctgttttt tacctttcag
    13261 atgatgatgg gcataagtcg cctcatgata tgtctcctga taaaccatta ctccatcgag
    13321 gccaagctgt tttaactcgg catattccgc ctccgccagc ggttgcactt ccatctgtag
    13381 tgaagagaac tgttcacgca gggcagggag atgacgacga aagtaatcca tccccacttt
    13441 cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc gtatagcggc
    13501 gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcggt tgctcataga
    13561 aaatccgcaa taagtacagt cgttagcgca aagattggaa agataaagcg ggacgtagaa
    13621 gctgacgacg ttgccaaatc gctgacgagt cagacgttgc gctcgctggg ccagtggttc
    13681 cagatagcca ctggcggcgg gtgatagcaa tgccatcatg tcgtcgcggg tgagttgcga
    13741 ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca ggtggatgtc
    13801 atcccagtcc agttgtcgcc agcgatcgct gaaggttttc atgccgatgc ctccagaaat
    13861 ccagtcagcg ggctggtggc atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt
    13921 gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac gggatcgtcc
    13981 gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag cgcctgcgcg
    14041 gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaacggtggc ctgctggata
    14101 ataatctcca gcatggcgcg ggtttccagt ccctgattcg agccaatcgg cgcgccgagc
    14161 ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg atcggccccg
    14221 cagtaaggca gcacgacaaa tccctgttgt accagcgttt cggcggcttt cagggtttcg
    14281 atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag ccagtttgtg
    14341 cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgcggt tttcgccccg
    14401 gaagtattcg gcagcagggc cacacccgcc gcgataagcg gttcgaggat agcgtcgttg
    14461 tgctggcgca agtcgacacg tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga
    14521 atagactcca ccatcagttg tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa
    14581 tcaaacgttt tgtccgcaat atgtaacatc tcaaccccct gcaataactt gaaaaagcag
    14641 gatctggtcg ccatcctgca cgatatgttg cgcccattgt tcccgcggga cgatttgctg
    14701 attaatcgcc agtgccgcac ctgcttgttg ttgttcgagt tgctccagca gttcgtgaac
    14761 agttagttcg gcgacacact gcatcggttg atcgttaaac aggatctgca ttgcatcctc
    14821 cacataccgg gcaaccgctg gcgcggcgca acgtcagact gcgccactgg ctcgatttac
    14881 cgtcgaacag tcggagttct cccgcaggcg tctctatacc acttaataac ttaatcgctt
    14941 ccagtgcctg taaagtgccc ataaccccga ccaccgggcc aaccacgccc gccgtgcggc
    15001 agttgcgttc tggctcctgg ttatctggcc acaggcagcg gtaacatccc tgctcccagg
    15061 gcggcgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg ataagcggcg
    15121 tgttgagtgc cacgcaggtg gcattaatct cctggcgagt cgccatattg tcagtacagt
    15181 cgagcaccac atcggcttgt gcaaccgcat cttttaacgt ctcacccgtt aaccgttgtt
    15241 gtaatgccat cagtcgaata tcgggattca actgtgtcag tcgctgttgg ctgacctgtg
    15301 atttcgggcg atcgatatct tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat
    15361 gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccccgcc aggtatagcg
    15421 cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg agcagttttt
    15481 gctgcccgtc cagagcgata tcgtcgagca ggatttggcg gctataacgc ataaagtcac
    15541 ggtcattcat cgccaactcc tgcaatttcc agcagctgtg ccgttgccaa acgccagtct
    15601 gcggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc tatcaccgca
    15661 ggcgcgcgcg ccagactgat accgccaatc gccacggtgg gataatccgc caggcgctca
    15721 acatgccgtg ccagctgttc cagtccctgc ggtgcagaag gcatctgttt ggtttgtgtc
    15781 ggaaacacat gtcccagcgc gatataagaa ggacgtgccg ccagcgcgac gtcgatctcc
    15841 atatcgtcat gagtcgaaac gcccagccgc aggcccgctg cacggatggc attgagatcg
    15901 gtggcttgca aatcttcctg ccccagatgg acgccatacg cctgatgctt aatcgccagc
    15961 cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc
    16021 accacatcgg cttctacctc ttcatcgcgc tgatctttga tgcgtagctg gagagtacgt
    16081 acgcctgcat ccaacagacg ttcgatccac tgtacgctgt caaccaccgg gtacagtcct
    16141 aaacgaaagg gtacaggagg aaactcaggc tgatacatca cgcttcctcc ttacgcaggt
    16201 agatttctcc gcctctggcg cggaagttct ccgacatatc cgccattccc acttcaatgg
    16261 tttgcgcggc ggcgtaatca cgcacttcct ggctgatttt catcgagcag aatttcggtc
    16321 cacacatgga gcaaaaatgg gcgactttac cggactcttg cggcagggtt tcatcgtgat
    16381 aagcgcgggc agtaaacggg tcgagggcca gattaaactg gtcttcccag cgaaattcga
    16441 agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt
    16501 cggcggcgtg agcggcaatc ttataggtga taagcccctg tttaacatct tctttatttg
    16561 gcaggcccag atgctctttc ggcgttacgt agcagagcat cgcgcagcca aaccagccaa
    16621 tcatcgccgc gccaatccct gacgtgaagt ggtcatagcc cggcgcgata tcggtagtta
    16681 gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc
    16741 ggcgaatcat ctgcatcggc acgtggcctg ggccttcaat catcacctgc acgtcatatt
    16801 cccaggcgat tttggtcagt tcgcccagcg tatgcagctc ggcaaactgt gcttcatcgt
    16861 tggcgtcctg aatagaaccg gggcgcaggc cgtcgcccag cgacagcgaa acgtcataag
    16921 cagcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc tgatgatggg
    16981 agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc aggcgtttcg
    17041 cggtcatcgg cacgtagcgc agcagcaccc ccgcatggat agtgaagtaa tccacacctt
    17101 gctcggcttg ttccagcagg gtgtcgcgga agacttccca ggtaagatct tcagcgatcc
    17161 cgttcacctt ctccagtgcc tggtagatcg gcactgtacc gatcggcacc gggctgttac
    17221 gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccagt ggaaagatcc atcacggtat
    17281 ccgctcccca gcgcgtggac cataccagtt tttccacttc ttcttcgatg gaagaagtga
    17341 ccgccgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca ataatcatcg
    17401 gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca gcaacttcat
    17461 cacggacaaa ttccgcagtg atattttccg gcagacgtgc gccaaagctc attcccggat
    17521 gctggtggcg taaaacttcg ctacgtatgc gctcgcgacc catattctcg cgaatggcga
    17581 tgaattccat ttctggcgtg atgataccct tgcgggcgta gtgcagttgg gtgacgcggc
    17641 gtcctgcttt ggcgcgtttt ggcgttaata cgccgctaaa acgcagctcg tcaaggccat
    17701 catctgccag ccgcgcttta gtgtaatcgg aactgcgtac ggtaagttct tcggtatcgc
    17761 cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg ttaatggcaa
    17821 tctgcggatc gccatacggg ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt
    17881 actgcggctg ttctttgctg ccgccaatta gcgtcgggct aagctggatc tcacgcatcg
    17941 gcacgcgcac gccggggtgt gtgccagtga tgtaaatgcg ttttgagttg ggaaaggcgg
    18001 agccttccag ggtgtcgata aaatgttggg cctgggcgcg ctgttcgcgg cgggtcagtt
    18061 ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga cgacggatga
    18121 agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt tcccttcgca
    18181 ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca gttaaggcac
    18241 tccgacaaga aattagcccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc
    18301 agaactcaag caggatattt gacgcgggcg gcgtcaagca ccagtagaat cagcttatct
    18361 tccagcacaa agcgcgcttc cagtgcctcg ccgatgtcag ataaaacctg ttgaaactca
    18421 aggtaattat catgatcgat ggcggtttcc aggctggaat cgtagtcatc cataatctgt
    18481 tgagtattgg cttcgagttg cggccaaatc ttcgcggcgc gcgcgagttg cccgttgcct
    18541 tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc
    18601 aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca
    18661 ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag
    18721 cgatcaacca gtttgttact tcctctgacg cgctccgtca ggttatcgag ctggttaagc
    18781 atgattgact ccgcaagttt gtattcaaaa actgctctgt gagaaatgta aaaaccatgt
    18841 taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta
    18901 gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg
    18961 ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa
    19021 tggcaggggg aacctgtttg gttagtacaa cagcagcggc gtcacgatat ggggtcggta
    19081 cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg
    19141 gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc
    19201 aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc
    19261 ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc
    19321 catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggtga aaccctcgag
    19381 caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttac
    19441 gtgacctccc aaccgtggcc gtttcctcag tcgttaatga ccgcgtttat ggcggaatat
    19501 gacagcggcg agatcgtgat cgacccgaaa gaattgcttg aggcgaactg gtatcgctat
    19561 gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat agaagatacg
    19621 gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac gcactaagga
    19681 acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc
    19741 gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa
    19801 gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaatgccga gctggcgtgc
    19861 gaagtgactt tgcaaccgct gcgtcgctat ccgctggatg cggcgatcct cttttccgat
    19921 atcctcaccg tgccagacgc gatgggttta gggctctatt ttgaagccgg agaaggtccg
    19981 cgttttacct cgccagtcac ctgcaaagcc gatgtcgata aactgccaat tccggacccg
    20041 gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc
    20101 gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcgaccta catggtggaa
    20161 ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga tgtatgccga tccacaggcg
    20221 ctgcacgcgc tactcgataa actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt
    20281 aaagccggtg ctcaggcagt gatgattttc gacacctggg gcggcgtgct taccgggcgc
    20341 gattatcaac agttctcgct ctattacatg cataaaattg ttgatggttt actgcgtgaa
    20401 aacgacggtc gccgcgtacc ggtcacgctg tttaccaaag gtggcggaca gtggctggaa
    20461 gcgatggcag aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat
    20521 gcgcgccgtc gcgtgggcaa taaagtcgcg ctgcagggta atatggaccc gtcgatgctg
    20581 tatgcgccgc ctgcccgcat tgaagaagaa gtggcgtcta tacttgccgg tttcggtcac
    20641 ggcgaaggcc atgtctttaa ccttggtcac ggcattcatc aggatgtgcc gccagaacat
    20701 gctggcgtgt ttgtggaggc agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg
    20761 attatggatc tcgcgtcatt acgcgctcaa caaattgaac tggcttcttc tgtgatccgc
    20821 gaggatcgac tcgataaaga tccgccggat ctgatcgccg gagccgatgt cggatttgag
    20881 cagggcggag aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg
    20941 gtggagtata aagtggcccg catcgccacc accatgcctt acattccagg ttttctctcg
    21001 ttccgcgaat atcctgcgct gctggcagcg tgggagatgc tgtcgcaaaa gccggattta
    21061 gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtagc cagccatttt
    21121 ggcttattgg tggatgtgcc gaccattggc gtggcgaaaa aacggctctg cggtaaattc
    21181 gaaccgctct ccagcgaacc gggcgcgctg gcccctctga tggataaagg cgagcagctg
    21241 gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg
    21301 gtcagcgtgg acagcgcgct ggagtgggta caacgctgca tgaaaggcta tcgtctgccg
    21361 gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca
    21421 gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga actcaacatg
    21481 ttacaaaacc caattcatct gcgtctggag cgcctggcaa gctggcagca tgtcactttt
    21541 atggcctgct tatgcgaacg catgtacccc aattacgccg tattctgtca gcaaaccggc
    21601 ttcggcgatg ggcagattta ccgccgcatt ctcgatctca tttgggaaac gctgaccgtc
    21661 aaagatgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca
    21721 gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcttgcgt ggcgttaagt
    21781 gaactggttc attcgcgttt aagtggcgaa acgctcgaac acgcggtgga agtgagtaag
    21841 acctccatca cgaccgttgc gatgctggaa atgactcagg ctggtcgcga aatgagcgat
    21901 gaagagctca aagaaaaccc agctgtagag caagaatggg acattcagtg ggaaatattc
    21961 cgacttttag ctgagtgtga agaacgcgat atcgagctga taaaaggcct tagggcagac
    22021 ctgcgtgagg cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg
    22081 tgatttaacg cctgatttgt cgtacctgga gtcttccctt tcgccccccg tctggtctac
    22141 atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc
    22201 atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac
    22261 aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa
    22321 gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta
    22381 caactggttg gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac
    22441 ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc
    22501 aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg
    22561 gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcagg tgcgctgttt
    22621 ctcactgcct gtagtcacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa
    22681 gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tcttcttgcc
    22741 gtgtttagcc cgtggcgcag tggcgatacg acgacgcgag agtatcgctg gcagggcgat
    22801 aacctcacgc ttatcaatat caacgtttac agcaaaccgc cggtaaacat ccgggcgcgt
    22861 tttgacgatc gcggtgactt gagctttatg caacgtgaat ccgacgggca aaagcagcag
    22921 ctttccaatg atcagatcga tttataccgt tatcgtgccg cgcagatccg cgagattagc
    22981 gatgccttac gccaggggag agtcgtgctg cgccaggggc gctggcatgc gatggagcag
    23041 actgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg
    23101 catatcgagc gccgccagag ccgttcttct gttgatgtca gcgtggcatg gctggaagcg
    23161 cccgaaggtt cgcaattact gttggtggca aactctgatt tctgtcgctg gcaacccaac
    23221 gagaaaacgt tctgatttac cagtggccca tacccatatg accgccacca ccgcagccgc
    23281 cgtaacccat tccggtaccg cgcggaatac ccgcttcagc catcgcaata tctcgtttca
    23341 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    23401 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc tttgtcacca
    23461 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    23521 tttgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    23581 caaatgccga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    23641 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttt
    23701 cgtgccaacg gcgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    23761 actcgcctgt tgtgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    23821 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgggca
    23881 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    23941 cgcgatcctc cccgtggtca ttgttgggct ggtggggctg tttgcggtga cagtgattcg
    24001 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24061 tatccgtgct ctcgaatccg gctcgcgcgt cggaatgggg atgcgcatgc atcatgcgca
    24121 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24181 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24241 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    24301 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    24361 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caatttttat
    24421 cgctttcgac gccagtaaca ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    24481 tatcctcttc gccctggcga cggtcttgct ggcaagcgtg ttgtcattct tctggtatcg
    24541 ccgatatctg cgctcgggcc agctgttgca ggatgaaatg aagcgcaaag agaagctggt
    24601 ggcactgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    24661 taaagggctg gcgaaatact ttgccgaacg cgcgccagca gggggagaag cgcatcaatt
    24721 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agctgctgga
    24781 actggttaag ccaacgcatc tggctttgca ggcggtggag ctcaacacgc tgattagcca
    24841 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    24901 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    24961 tctctatctc aacgctattc aggcgattgg tctgcatggc gtgattagcg tgacggccag
    25021 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcgga
    25081 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggactggg
    25141 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg ttgcaagcca
    25201 ggaggggaaa ggcgcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25261 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    25321 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    25381 cgacaggcgc tggagcaggt gcgggaacag gttttcgatc ttgtgctttg cgatgtgcga
    25441 atggcagaga tggacggcat cgccacgcta aaagagatca aagcgttaaa tccggcgatt
    25501 ccggtactga ttatgaccgc gtactccagc gtcgagacgg cggtagaggc actgaaaacc
    25561 ggggcgctgg attacctcat caagccgctg gatttcgata acctgcaggc aacgctggaa
    25621 aaggcgctcg cgcatacgca cagtatcgac gctgaaacgc ctgcggtgtc tgccagccag
    25681 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    25741 gcgccatcgg aagccacggt gctgatccac ggcgattccg gtaccggtaa agagctggtc
    25801 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    25861 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcatgaaaa aggggcgttt
    25921 accggagccg acaaacggcg agaagggcgc tttgttgagg cggacggcgg cacgctgttt
    25981 ctcgatgaga ttggcgatat ctcgccgatg atgcaggttc gtctgctgcg tgcgattcag
    26041 gagcgcgaag tgcaacgtgt cggcagcaac cagactatct cggttgatgt ccggctgatt
    26101 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26161 tactatcggc tgaacgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26221 attcctctgc tggctggcca ttttcttcag cgctttgccg aacgtaatcg caaggcggta
    26281 aaaggcttta cgccccaggc gatggatctg ctgattcatt acgactggcc gggaaatatt
    26341 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    26401 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggtaca aagtcaggat
    26461 attcagccgc tggtggaagt cgaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    26521 ggcaacaaaa ccgaagccgc ccgccagtta gggatcacgc gcaaaacgct attggcaaaa
    26581 ctgtcgcgtt agttctgctc gcgttcgata gcgcgccagc cgatatcttt ccggcagaag
    26641 cagtcgtccc aatggatatc ggtcattaag gcataagcgc gtttctgcgc ttctgccacg
    26701 gtatgaccca gcgcggtaac gcacagtacg cgcccgccgc tggtcactac ctgctcgtca
    26761 tccgccagtt tcgtgcccgc gtggaacact ttgccgtctg ccacttcttc cagcggcagg
    26821 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    26881 ccgagagaag cgcgttcatc ccactcggac gttttctcgt ccagcttgcc ttcacaggcc
    26941 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27001 tcgccaaagc ggcagttaaa ttcaataacc tttggattac cctgtttgtc gatcatcagg
    27061 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27121 cagatgatac gttccatggt gcgctgatga acttcatcgg tcactaccgg cgcaggagag
    27181 taagccccca ttccgccggt gtttggcccg gtatctttat cgcctacgcg tttgtgatcc
    27241 tggctggtgg ccatcggtag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    27301 tcaccgtcga ggaactcttc gataacaata cgatgacccg cgtcgccaaa cgcgttgccc
    27361 gccagcatat cgtgaacagc tgcttcagct tcttccagcg tcatcgccac gataacgcct
    27421 ttcccggctg ccagaccgtc agctttaatg acgattggcg cgcctttctc acgcagatat
    27481 gccagcgcag gttctacctc ggtgaagttc tggtattccg ctgtgggaat gttatggcgg
    27541 gccagaaagt ctttggtgaa tgctttagaa ccttccagtt gtgcagcacc tgcggttggg
    27601 ccgaagattt tcagcccggc ggcgcggaag gtatcgacta cgcctttcac cagcggcgct
    27661 tccgggccga cgatggttag atctaccttt tcgttttgtg cgaaatccag cagcgccggg
    27721 atatcggtta cgccgatagc gacgttctgt agcgcgggtt ccagcgcagt gcctgcattg
    27781 cccggagcaa caaaaacagt ttcaaccagc ggcgactgag ccgctttcca ggccagcgcg
    27841 tgctcgcgcc cgccgttacc gatcactaat actttcatct gtcgctccat taatggcgga
    27901 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaattactt
    27961 cgtcatcacg gatagaaccg ccaggctgga ttacgcaggt cacgcccgca gcggctgcgg
    28021 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc cattgacgaa cctttcactt
    28081 ccaaaccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28141 ggcctgcgcc aataccgatg gtcatattgt ttttggcata gacgatagcg ttggatttca
    28201 cgaacttcgc cactttccag cagaacagcg catcacgaag ttcctgttca gtcggctgac
    28261 gtttggtgac gacgcgcagt tcttccgcac cgaccatccc cagatcgcga tcctgaacca
    28321 gtagaccgcc gttcacgcgt ttgaaatcaa gacccggaat acgttcgccc cactgaccgc
    28381 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    28441 acggcgcaat aatcacttcg acgaactgac gagaaatgat ggcctgcgcg gtttcagcat
    28501 ccagttcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    28561 cgcgatcgta agcatcaaga atggaattgc cgatagccac gccgcaaggg ttggcgtgct
    28621 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    28681 cggcgatgtt gttataagag agagctttac cctgaacctg ggttgcggta gcaacggagg
    28741 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    28801 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    28861 ctttgctttc accgtggtaa gccggaacca tgctgccgaa gtagttggca atcatgctgt
    28921 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    28981 gcgatccttc gttggcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29041 cgattgcgac atctttatgg ttcttggcgg cggagcgcac catcgttggg ccgccgatat
    29101 cgatgttctc taccgcatct tccagcgagc agccttcacg agcgacggtc tgggcgaacg
    29161 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29221 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    29281 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacatt
    29341 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    29401 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    29461 ctggacgacg ttgttgcatg gtaaatcccc tggatttgac tattacagag agcgttagct
    29521 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    29581 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    29641 ttttgacagt ggctaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    29701 tgtgtgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    29761 tgcaattttt ctattgcggc ctgcggag
//
LOCUS       DACADA010000045        29398 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.045, whole genome shotgun sequence.
ACCESSION   DACADA010000045 DACADA010000000
VERSION     DACADA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.045"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            62..1645
                     /locus_tag="ISB17_001980"
     CDS             62..1645
                     /locus_tag="ISB17_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1979914.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2097..3950)
                     /gene="asmA"
                     /locus_tag="ISB17_001981"
     CDS             complement(2097..3950)
                     /gene="asmA"
                     /locus_tag="ISB17_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAP1979915.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3972..4553)
                     /gene="dcd"
                     /locus_tag="ISB17_001982"
     CDS             complement(3972..4553)
                     /gene="dcd"
                     /locus_tag="ISB17_001982"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAP1979916.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4645..5286)
                     /gene="udk"
                     /locus_tag="ISB17_001983"
     CDS             complement(4645..5286)
                     /gene="udk"
                     /locus_tag="ISB17_001983"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP1979917.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5604..8921
                     /locus_tag="ISB17_001984"
     CDS             5604..8921
                     /locus_tag="ISB17_001984"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1979918.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGKINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     SPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9028..9876)
                     /gene="alkA"
                     /locus_tag="ISB17_001985"
     CDS             complement(9028..9876)
                     /gene="alkA"
                     /locus_tag="ISB17_001985"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAP1979919.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSVGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10010..11362
                     /gene="yegD"
                     /locus_tag="ISB17_001986"
     CDS             10010..11362
                     /gene="yegD"
                     /locus_tag="ISB17_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1979920.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11375..13315)
                     /gene="yegI"
                     /locus_tag="ISB17_001987"
     CDS             complement(11375..13315)
                     /gene="yegI"
                     /locus_tag="ISB17_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAP1979921.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTS
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPSGLLRREYI
                     ILIEIALSGLSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKVEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13312..14073)
                     /gene="pphC"
                     /locus_tag="ISB17_001988"
     CDS             complement(13312..14073)
                     /gene="pphC"
                     /locus_tag="ISB17_001988"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HAP1979922.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQIQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLALNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14070..14729)
                     /locus_tag="ISB17_001989"
     CDS             complement(14070..14729)
                     /locus_tag="ISB17_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1979923.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGSAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14947..15003)
                     /locus_tag="ISB17_001990"
     CDS             complement(14947..15003)
                     /locus_tag="ISB17_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP1979924.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(15275..15331)
                     /locus_tag="ISB17_001991"
     CDS             complement(15275..15331)
                     /locus_tag="ISB17_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP1979925.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15610..16857
                     /gene="mdtA"
                     /locus_tag="ISB17_001992"
     CDS             15610..16857
                     /gene="mdtA"
                     /locus_tag="ISB17_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAP1979926.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKAASREDTKKGARS"
     gene            16857..19979
                     /gene="mdtB"
                     /locus_tag="ISB17_001993"
     CDS             16857..19979
                     /gene="mdtB"
                     /locus_tag="ISB17_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAP1979927.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19980..23057
                     /gene="mdtC"
                     /locus_tag="ISB17_001994"
     CDS             19980..23057
                     /gene="mdtC"
                     /locus_tag="ISB17_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAP1979928.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            23058..24473
                     /locus_tag="ISB17_001995"
     CDS             23058..24473
                     /locus_tag="ISB17_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1979929.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24470..25873
                     /gene="baeS"
                     /locus_tag="ISB17_001996"
     CDS             24470..25873
                     /gene="baeS"
                     /locus_tag="ISB17_001996"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAP1979930.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25870..26592
                     /gene="baeR"
                     /locus_tag="ISB17_001997"
     CDS             25870..26592
                     /gene="baeR"
                     /locus_tag="ISB17_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAP1979931.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26772..27104
                     /locus_tag="ISB17_001998"
     CDS             26772..27104
                     /locus_tag="ISB17_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP1979932.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27251..28612
                     /locus_tag="ISB17_001999"
     CDS             27251..28612
                     /locus_tag="ISB17_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAP1979933.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29112..>29398)
                     /locus_tag="ISB17_002000"
     CDS             complement(29112..>29398)
                     /locus_tag="ISB17_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979934.1"
                     /translation="IFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLA
                     RGRISGAENIVKNDAAHFCVTQGKKMQIVKLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6788 a         7875 c         7754 g         6981 t
ORIGIN      
        1 ccgatttatc catgatcgaa ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac
       61 tatggaatgg attgccgatc cgtctatctg ggctgggtta atcacgctga ttgtgatcga
      121 actggtcctc ggcattgata acctggtctt tattgccatc ctcgccgaaa aactgccgcc
      181 gaagcagcgc gaccgcgcac gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt
      241 actgctggcg tcaatctcct ggctggtcac cctgactcaa ccgctgttca gcttccgctc
      301 gtttaccttt agcgcccgcg atttaatcat gctgtttggt ggtttcttcc tgctgttcaa
      361 agccacgatg gagctgaacg aacggctgga agggaaagac agcaataacc ccacgcaacg
      421 caaaggtgcg aagttctggg gggtggtgac acaaattgtg gtactggacg ccatcttctc
      481 actcgactcg gtgattaccg ccgtcgggat ggtcgaccat ttactggtca tgatggccgc
      541 cgtggttatc gctatcagcc tgatgttgat ggccagcaag ccgttaacgc aatttgttaa
      601 cagtcacccg accatcgtta ttctctgcct aagcttcctg ttgatgattg gctttagcct
      661 ggtggcagaa ggtttcggct tcgtcattcc gaaaggctac ctgtacgctg ctattggttt
      721 ctcggtgatg atcgaggcgc tcaatcagtt ggctatcttt aaccggcgac gttttctttc
      781 cgctaatcag acgctgcgcc agcggactac cgaagcggta atgcgcctgc ttagtgggca
      841 aaaagaagat gcggaactgg acgccgaaac cgcgtctatg ctggtggatc atggtaacca
      901 gcaaatcttt aatccgcagg aacggcggat gattgagcgg gtacttaatc ttaaccagcg
      961 taccgtcagc agcattatga cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc
     1021 ggaagacgag atccgccagt tactggagcg aaatcagcat acgcggcttg tcgttactga
     1081 tggcgatgac gcagaagatt tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc
     1141 actgcgcggc gaaccgctca acctgcgggt gttgattcgc cagccgctgg tgttcccgga
     1201 aaccttgccg ttgttacctg ccctggagca gttccgtaat gcccgcacac actttgcttt
     1261 tgtggtggat gagtttggct cggtggaagg gattgtgaca ttaagtgacg tcactgaaac
     1321 cattgccggt aacttaccga acgaagtgga agagatcgac gcccgccatg atattcagaa
     1381 gaatgcggac ggttcctgga cggcgaatgg tcatatgccg ctggaagatc tggtgcaata
     1441 tgtgccgttg ccgctggatg aaaaacgtga atatcacacc attgccgggc tattgatgga
     1501 atatttgcaa cgtattccaa aacctggcga agaagttcag gtgggggatt atttgcttaa
     1561 aacgttgcag gtggaaagcc atcgcgtgca gaaggtgcag attattccgc tgcgtaagga
     1621 tggcgagatg gagtacgagg tgtgatggca tcggccattt tacccaatgc tgcgtaacgc
     1681 cttataactt cagcaatgaa gcgttgtctg tggtacatgg acatcacagc gggggtgtgg
     1741 gtccggctgt aaatcgccga atcgtttcct gtaggccggg gcagcccaca tggatgtggg
     1801 ctgagggcgc gttttacagg gatgttacct cgcgcccgta cccgatagcc gaaaggaata
     1861 agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg
     1921 gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc
     1981 aagtgaacgg aacagccttc gatcttacat attcagaata taatggtgcc gccacgaacg
     2041 cctgatgcga cgctttacgc gtcttatcag gcctacagga caggtcacct ggtaacctac
     2101 atcttctcca gcaacttctt cacatctttg ccattgcggg aatctttatt ccgctcggcc
     2161 cagtcgttaa gacggcgttt tgcttcatcc tgtagatgtt tacgcagtaa ctgatccact
     2221 tgcaggctgt aattaagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct
     2281 ttgaggaaat caatcagttt gctttccccg ttccagccac ccacgacccg aatatcaaac
     2341 tgggtgtcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag cgccagcaca
     2401 ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg
     2461 gtaaagcggt caagacgcgt cacgttatcg aagttttcag cggccttcac atcaccacca
     2521 ttacgttcta ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgtgtatca
     2581 gtcatttcga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca
     2641 gcaccggaga aatcaccagc cagtgacatc tttccggtca acgaaatcgg atagttaaac
     2701 gccttcagaa tggtgccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga
     2761 tttattgatg tcgcgtccaa cgtgcccggc agtgaaacct gtccaccgtt aagtttgccc
     2821 tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta
     2881 aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct
     2941 tgcagtccct gataggccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt
     3001 gattggctct gcccttgctg cgcggcaccg ttctcaccgt tcgctgtttc attaaggggg
     3061 atgaggttgt caagattcag ttgcgggaat tgcagtctca gctgccattc cggtttctct
     3121 gtcaacgtga cctgtgcttg cccgctcagc gtactgtcat tggcggtcaa actgatttgg
     3181 ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga
     3241 ataccttgtt ttggtaaatc ggccccctgc aactgccagt taatttgttc aatagccgcc
     3301 gttaaatcat gcggataatc agaagcatct actgtaccgt taagggataa tgtgagatcg
     3361 cgctgatcgc gattaacccg cccggaaaac tcaaatgagc cacgatgttg gggatcttgt
     3421 tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc
     3481 agaacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac
     3541 agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc
     3601 gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga
     3661 tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc
     3721 tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag
     3781 acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc
     3841 ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg
     3901 gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca
     3961 atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat
     4021 ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggagagcgg ctcaaagctc
     4081 agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtaaaac
     4141 tccagcacaa tgcagccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc
     4201 agccccagac gcgccagtga agaacgcccg tccagccagc ccaccagatc ggctggcagc
     4261 gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata aaacgcctcg
     4321 ccctcgtcga gcacgatttc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct
     4381 ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg
     4441 cccaggcgca catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc
     4501 gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt
     4561 tctcctttcg agcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac
     4621 tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat
     4681 atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga
     4741 aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcactga
     4801 atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca gacagatatc
     4861 cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgtaaacgcg catccgtcag
     4921 caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat
     4981 acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc
     5041 ttgtaaatgc tcaagcagca ggctgtgatc catcgcgctg ggatggtcgt agttggtctt
     5101 aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac
     5161 gccgatgtgt tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag
     5221 actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc
     5281 agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa
     5341 acgcggcaat tatagggatt tcatccgcct gataccagtc gaatagcgtt gccgcgcgct
     5401 cagagttaat tgttgacgaa gaattcccgg tggcaaatta cgttgatcag ttttatacaa
     5461 ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa
     5521 ttcgcatata aaatgtaaaa cttttgtact agcatgaaca cagaaacgaa tactgacgac
     5581 ctggccttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc
     5641 cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttc acccttttct
     5701 cacttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg acgtccatca
     5761 tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctgagctgct
     5821 cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct
     5881 ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc
     5941 cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcactc ggcagcgcca
     6001 ttattccgcc tctgttgggg ggtgttctgg ttgtcctgct gacgcccgga gacgatcctc
     6061 tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca ctggtgccgc
     6121 tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccaagg ttgctttttg
     6181 agtcgctgct cacgttagct atcacgctga cgttaagctg gctttcgatg ctgtatctgc
     6241 cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgc ctgccacgaa
     6301 tggaagcctt tttgatcttc cttaccacag tgatgatggt atcgctgatg atggccgcgg
     6361 atccctctct gcttgctacg ccgcgtacgt acctgatgag tcatatgccg tggctaccgt
     6421 ttttgctgat cctgctgccc gccaacatca tgaccatggt gatgtatgcc tttcgtgcgg
     6481 aacgcaaaca catttccgaa agcgaaaccc gctttcggaa cgcgatggaa tattccgcca
     6541 tcggcatggc attagtgggc accgagggac aatggctgca atccaacaaa gcgctctgcc
     6601 agtttctcgg ttacagtcag gaagaactgc gcggactcac ctttcagcaa ctgacctggc
     6661 cggaggatct caataaagat ctgcaacagg ttgaaaagct gatcagcggt aaaataaaca
     6721 cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg gcgttgcttg
     6781 ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag
     6841 acattaacga gctaaaacgc accgaacagg tgaaccagca actgatggag cgcatcacgc
     6901 tggctaacga agcgggcggg attggcatct gggagtggga gctaaagccg aatattttta
     6961 gctgggataa gcggatgttc gagctgtatg aaattcctcc gcatatcaaa ccgaactggc
     7021 aggtgtggta cgagtgcgtg ctgccggaag atcgccagca cgccgaaaaa gtgattcgcg
     7081 attcattgca atcacgctct ccctttaaac tggaatttcg cattaccgtg aaagacggca
     7141 ttcgccatat ccgcgccctc gctaaccggg tactgaataa agaaggcgaa gtcgaacgcc
     7201 tgctcggcat taatatggat atgaccgagg ttaaacagct taacgaggca ttgtttcagg
     7261 aaaaagagcg cctgcacatt acgctggatt ccatcggtga agccgtggtc tgtattgata
     7321 tggcgatgaa aattaccttt atgaatccgg tggcggagaa gatgagcggc tggacgcagg
     7381 aagaagcgtt aggtgttccg ctcctgacgg tgttgcatat tacttttggc gacaacggac
     7441 cattaatgga gaacatttac agtgccgaca cctcacgttc cgcgattgaa caagatgtgg
     7501 tgttgcactg ccgaagcggc ggcagctacg acgtgcatta cagtattacg ccgttaagta
     7561 ctctggacgg cagcaatatt ggttcggttc tggtgattca ggacgtcacc gaatcgcgca
     7621 aaatgctgcg ccagctgagc tacagcgcct cccatgatgc actgacgcat ctcgccaatc
     7681 gagccagttt tgagaagcaa ctacgcatcc tgctgcaaac ggtaaacagt acacatcagc
     7741 gacatgcact ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac agcgccgggc
     7801 acgccgctgg cgacgcttta ctgcgcgaac tggcgtcatt gatgctgagt atgctgcgct
     7861 ccagcgacgt gctggcgcga ctcggtggcg atgaatttgg tctgctgctg ccagattgta
     7921 atgtcgaaag tgcgcgtttt atcgctacac gcattatcag cgccgtgaat gactatcact
     7981 ttatctggga aggacgtgta catcgggtag gtgccagtgc agggattacc ttgattgatg
     8041 acaacaatca tcaggcggct gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca
     8101 aaaatggtgg ccggggccgg gtgacgattt acgaaccgca gcaagttgcc gcacatagcg
     8161 aacgggctgt gatgtcgctt gatgaacagt ggcggatgat taaagagaat cagttgatga
     8221 tgatcgccca cggtgtcgct tcgccgcgga tcccgcaagc gcgtaatttg tggctgattt
     8281 cacttaagct ctggagttgc gaaggcgaga ttattgatga acaaacattt cgtcgtagct
     8341 tcagcgatcc ggcacttagc catgctcttg accgacgggt attccacgat tttttccagc
     8401 aggccgcaaa agcgattgcc agtaaaggct taagcatcgc cctccccctt tccgttgccg
     8461 gtttgagtag cgccacgctg gtgaatgaac taattgagct gctggaaaat agccctctat
     8521 caccacggtt attacatctg attattccgg cagacgcgat tttagatcac gcagaaagcg
     8581 tgcaaaaact gcggctggcg ggatgtcgga tcgtattcag tcaggtgggc cgcgatctgc
     8641 aaatcttcaa ctcgctgaaa gcgaatatgg cagattacct gctacttgat ggtgagttat
     8701 gcgccaacgt gcagggaaat ttgatggatg agatgctgat tacgatcatt caggggcacg
     8761 ctcagcggct cgggatgaag accatcgccg ggccagtcgt tttaccctta gtgatggata
     8821 cgctttctgg catcggcgtc gatctgattt atggcgatgt gattgccgat gcccaaccgc
     8881 tggatttgct ggtgaatagc agttatttcg cgattaacta agggagcgtt tgcccattgc
     8941 ctgatgcgac gctaacgcgt cttatcaggc ctacaaatcg ctcattcccc aggccggata
     9001 agctcgcacc gcatccggca accaacgtca tgcttcgtct ggttgccagc cttccgtata
     9061 ccagatatgc aacaacgcat aagaacgcca gggcttccag cgttcggcat aacggcggat
     9121 ttgtgccggt gtcattcccg gaaatcgctg tttaatcaga taatcatccg gcagaaaaac
     9181 atctttcgcc tgccagccac gcaaagcaaa ataatttgcc gtccagcgcc cgatacccgg
     9241 aaaggtttgc agcgttttca tcgcttgttc tacgtcgccc ggtattgtca ttggtaaggt
     9301 gccctccagc gccgcatttg ccagatgaat cagcgcctct gcccgtttca acggcatacc
     9361 taacgctttt aatgcctgcg ggtcggctac tgccagccgc tgaggcgtgg ggaagcagac
     9421 ataatccgga aaatcatcca gccgttcgcc ataaagctgt gccactctgg aggtcaattt
     9481 tgccgccatc gccacgctca ccagttggcc taaaatcgcc cgcacgccct gctcaaaagc
     9541 gtcaacacaa ccaggtaaac gcaatccggg ccgccccgcg cctaactttc ccaacgcacc
     9601 gttaacaatc tgcgggttac attgcagatc aaacaggcgg ctcattttcg ccagacactc
     9661 agcggcaaca ggttctaaac ctacacttaa atttatgtgc agagtatggc gggctatatc
     9721 cggaatagca gtcaccacgc cgcgatattc gcctaccgcc agactacggg cataataact
     9781 gtccgcgacc gtttccacgc cgctcaccgc acgggcggcg agaaatccca acatccacga
     9841 ccagtcatac ggcggctgcc agttcagggt atacatcgca tctccttgtt aatccgcttt
     9901 cagcataaac gttattcaga cgttacgctt tgctttcata ttccggttgt cgcgacggca
     9961 acatttcgct aaagtcacgc cccttcttca ccggcatggg gattatttcg tgtttattgg
    10021 ttttgattac ggtacagcaa actgttcagt ggcggtcatg cgcgacggta aaccgcattt
    10081 gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga
    10141 agcggtaagc gaatggctgt accgccatca tgatgttccg gcagacgacg atgaaacgca
    10201 ggcgctgttg cgtcgggcga ttcgttataa ccgcgaagaa gatatcgatg tcacggcgaa
    10261 aagcgtgcag ttcggtcttt cttcactggc acaatacatt gatgatccgg aagaagtgtg
    10321 gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc
    10381 gctgtttgag gatctggtct gcgcaatgat gttgcacatt cgccaacagg cgcaggcgca
    10441 gctgccggag gcaattactc aggcggtgat tggtcgcccg atcaacttcc aggggctggg
    10501 cggtgatgaa gcaaacaccc aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg
    10561 gtttaaggat gtggtattcc agtacgagcc ggtcgcggct gggctggatt acgaagccac
    10621 cttgcaggaa gaaaaacgag tgctggtagt ggatatcggc ggtggtacga ctgactgttc
    10681 attgctgctg atggggccgc aatggcgttc gcgtctcgat cgtgaagcca gcctcctggg
    10741 tcatagtggt tgccgtattg gtggtaacga tctggatatc gcactggcgt ttaagaacct
    10801 gatgccgtta ctgggcatgg ggggcgaaac cgaaaaaggc atcgccctgc cgattctgcc
    10861 gtggtggaat gcggttgcca tcaacgacgt acctgcgcaa agtgatttct acagtagtgc
    10921 caacggtcgt ctgcttaacg atctggtacg cgatgcccgc gaaccggaaa aagtggccct
    10981 gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg gtacgtagcg cagaagagag
    11041 caaaattgcg ctttcaagcg tagcggaaac ccgcgcctca ctgccgttta tcagcgatga
    11101 actggctacg ctgattagcc agcaagggct ggaaagcgcc ctcaaccagc cactggcgcg
    11161 gattctggaa caggtgcaac tggcgctgga taacgcccag gaaaaaccgg atgttatcta
    11221 tctgaccggc ggtagcgccc gttcaccgct gattaaaaaa gcgctgacag aacagttgcc
    11281 gggcattccg attgcaggtg gcgatgactt tggctccgtc accgccgggc tggcacgctg
    11341 ggcggaagtg gtgtttcgtt aattgaaaac tggctcagca acggcttaaa tcagcctgcg
    11401 cctgagccag tttttctgcc gcctgacgta acggttccat caacggcatg gtccgtgctg
    11461 gagcttgaag gcggaatcgc tgcaactctg tcgcaccgac agtcagcgcc gattccagcc
    11521 gatgtcgttt ggcggccatt tttgccatta ccgcttgtct gtctgcaggc gttaccgctt
    11581 cgttcggcct gaaaacaaaa cggcgttcgc aactcgcttt ccagtcgata accgcctggg
    11641 tcagatgatc accaaaaccc ctaacttgct taacgctccg tcgggtaacg tccgctgctg
    11701 tttcaatacc aaaggaccgt aacgccgctt tacgcgcagg gccaacgccg ggaatagagg
    11761 caacatcaat aaaaaatccc tccagaaact tctgcttctg ccgttccctt gcggtgtcgt
    11821 gaagtgctgc cagatcgcgc ttttcttctt ccggtaaccc aagaatttcg tccttcattt
    11881 tttcgagcat cgcccgtttg gcgataaaac cttccagccc gcccaactgt tggatctggc
    11941 tgactaaatg gtcatagtcc atttttgcgc gattaaaagc ctctcttcgt cgctggactt
    12001 ctactttgta cgctttgctt gtcaggctgc caataatcca gatagccgcc agcacgggaa
    12061 ccaaaatgat ataacgcggt tctgtctgaa ggccgcaaag cagcagcgat aaccctgaca
    12121 gtgcgatctc aatcagaatg atgtattcgc gccttaacag gcctgaagga agcggcctgc
    12181 cagcagcctg gaaatgatcg ggtaatggca attgcaatgc tggcggtgct actgacgcca
    12241 tcaccatcgc ccagactttc gccagcacaa aattcccgcc ggtggtaatg acctcttcgc
    12301 cgagatcaat aaaatagata acaccttgat tatccagcgc acaccatggg cagtcagcca
    12361 aatgaccggg ataaacatgc attgccgaaa cggtacattt ctttaactgt tggcgtagta
    12421 aatcaagtgc cgctacccac gcttttgccg tcggacgccc ggttgccacg ccactttccg
    12481 tgaatgcctg ctgaaacatg gcttcaacat cgcccggtaa catcgacagc ggaatcgatc
    12541 gtggcggcgg ttttaaacca cgtcgctgat tatctgacgc gtaggcataa cggaaatggg
    12601 caatatccgt ctccagcgca ttacccgcat cagagataag cggtacaccg gaataaggat
    12661 gccgcccacc aaacaagacg tgaaatatca gcaacgcaag gccaaaatta tcgtgatttt
    12721 ctgtgcgttc aaagccgaca aatgatggca tggtttgcag ctctggcggc gtaaaatgcg
    12781 acacgccgac ttcgcataaa tgcagtgtac cattggcatt aatctgaaag gagtcgctat
    12841 caatcatcac cactttgctg tcgcgaccta ccataaagct gttctggttt acgtcaccca
    12901 cgacgtgccc gtgctcgtga accgtagcaa aagatgaagc aatattgcgc gcaacataga
    12961 gtagaaaatc ccacgcacaa tgagggtaac tctggcgacg atgtgccggg ctatagatca
    13021 tatgaatcgg ttctttacca gaaacttttg gcatcataaa accgataact tttccacctc
    13081 gtccaccgtg aagcgttgcc tgcggccagg cgacataatt caacaactgc gcgtcagctg
    13141 tcgcagccat aaaggcaagt ttgtcctgtt ttaaggcggg aggcgacgtg tgataaatct
    13201 tggcgacgct ctcgacaaac tcctcgatat cataaaccgc accttcgccg cctttgccca
    13261 gttcacggcc tggcgtaacg cactcaccag tagcagtata taaagtgggt ttcatggtaa
    13321 ccacagcgcc agtgccaacg ttttatcatc atccgtacgc tcgtttaccg ccgggctgga
    13381 caaaaactgc tttaacaatt caggtaataa atcgagttgt tcctgcgttg ctgacgccag
    13441 tccattgaaa aacggggtaa agaaaggcat atgcggagaa ttatccagca tatttaacgc
    13501 cagtcgctga ataccatccg taaatgctgc aactttgtgc gcacgctcag tgctggtgaa
    13561 agtatccagt ctggaaacgg catcttcatc agtaatgaag tgcgtcatat tggcatattc
    13621 ccccaccatc ggcgtgagcg gcagttgcaa accgtgaccc aaatctacca ccacgccgcc
    13681 atcgccaatc tgcataatca aggtgccatt agctgatgaa atcaatccca aaaacgtgca
    13741 ggcaaaatcg cgaaccgcta attctttggc ttccgcctcg gcaaaaagtc gctgacgaat
    13801 ggtcagcaca atgtccgttg cgaggacatc attaagcccc aattccccgc cctgcacttt
    13861 ttgcgacata taagccatcg cttcattgac ggcgagcatc gcaccttcgc ctccctgcga
    13921 ggcgctacct gcgccatcag cgacaaatac cgacaataat ggctgctggt catttagcca
    13981 ggcaacctgt atctggcagg catcctggca aggcaagtcc gcactgatgt gcgacgttcc
    14041 aacggctgat gcatagacca gtcgccagct cacactgacg tccagccttt tggcgcttcc
    14101 agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac gcagcgaact ggataaccag
    14161 ctaaacagtt cacggaattg taatccctgc agtggcaaag gctgacgaac gctgatttgt
    14221 gccagcgtct tcatatccgc cccctgaacg ccgatggtaa agaaggcaaa cttcttatct
    14281 tcttccccct gaaacacttt gttggcggca gcctgccact catcggttgg tgcaccatcg
    14341 gtgatcagga aaatccacgg acgataatag gaaataccat tggcacgata ttcacgtttc
    14401 cgctcctcaa ccatatccag ggctttggta atggcagagc ccattggtgt atcgccctgg
    14461 gcaaacagga tgggcgggaa aaaattagcg gcgctggtaa agggttgttc cacatgcacc
    14521 gggccgaacg tcacaatgcc gagttccact ctttttaagg ccagcgaatc ggcaagcagc
    14581 tcatcgcgaa aggtaaccaa tccggcgtta agttcattaa tgggtctacc gttcatagaa
    14641 ccagaaacat ccagcagcaa aatgcagggg caacgtggtt ccgggttact ggcaaaatcg
    14701 ctggtggcaa acgtgatttg ttcgctcata agaaatcctt tcttagagaa ttattgaggt
    14761 ccggtatcat accgactgca ataatgaaat aagagcaaac gaagaaactt tcagaattag
    14821 gcccggcggc gcagaagatt ttgctattcg cctcgcaaga acgtgggctt acagattgac
    14881 aacaagggtt gagtgacata atttcaggag tgagggtaga gcggggtttc ccccgccctg
    14941 gtagtcttag tatgctgggt agctaatgac taagagcacc acgatgatga gtagcttcat
    15001 catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
    15061 cgctgaaatc ttcggcttac ctcctttcgc cctgtatgca gtctatcgct gacttgcgga
    15121 taaaatagtt tcaagtgtga ttattgcgta acgactcgca aagaatattt gctggcagga
    15181 tcgcagactg caaagtctgc ggattgacaa ttggaccgtg aaggcatact ttcaggaatg
    15241 agggttgagc ggggtttccc ccgccctggt agtcttagta agccgggaag cttatgacta
    15301 agagcaccac gatgatgatc cacttcatca tgaccctttc cttatttatg gccccttcct
    15361 cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttatct cctttcgcca
    15421 tgcaagcagt ctatcgctaa cgcgtagata aaatagtttc atgtgttatt actggatgcg
    15481 tgctcgcaaa agtgcccgtc attcagacga ttcccgacag tgtttcataa ttcctccatt
    15541 tttctccctt attggctggc tacactagta tcattccgcg aaacgtttca ggaagagaaa
    15601 ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa tcgtaatcgt ggtggttatc
    15661 gccgccatcg ccgcattctg gttctggcaa ggccgcaatg actcccagag tgcagcccct
    15721 ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc gccgtggtat gcgtgccggc
    15781 ccattagctc ctgttcaggc ggcgaccgca gtagaacagg ctgttccacg ttacctcacc
    15841 gggcttggca ccattaccgc cgctaatact gttacggtgc gcagccgcgt ggacggtcaa
    15901 ctgatggcgt tacatttcca ggaaggccag caggtcaaag ccggcgattt actggcagaa
    15961 attgacccaa gccagttcaa agttgcatta gcacaagccc agggccaact ggcaaaagat
    16021 aaagccacgc tcgccaacgc ccgccgtgac ctggcgcgtt atcaacaact ggcaaaaacc
    16081 aatctcgtat ctcgtcagga actggatgcc caacaagcgc tggtcagtga aaccgaaggc
    16141 accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga ctggagccgt
    16201 atcaccgcac cagttgatgg tcgcgttggt ctcaagcagg ttgatgttgg taaccaaatc
    16261 tccagtggcg acaccaccgg gattgtggtg atcacccaaa cacatcctat cgatctggtc
    16321 tttactctgc cggaaagcga tatcgccacc gttgtgcagg cgcaaaaagc cggaaaaccg
    16381 ctggtggtag aagcctggga tcgcaccaac tcgaagaaat taagtgaagg cacactgtta
    16441 agccttgata accaaatcga tgccactacc ggtacgatta aagtgaaagc acgctttaat
    16501 aatcaggatg atgcgctgtt tcccaatcag tttgttaacg cacgcatgtt agtcgacacc
    16561 gaacaaaacg ccgtggtgat cccaaccgcc gccctgcaaa tgggcaacga aggccatttt
    16621 gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc tggtgacacc aggcattcag
    16681 gacagtcaga aagtggtgat ccgcgcaggt atttctgcgg gcgaccgcgt agtgacggac
    16741 ggaattgatc gcctgaccga aggggcgaaa gtggaagtgg tggaagccca gagcgccact
    16801 actccggaag aaaaagccgc cagccgcgaa gacacgaaaa aaggagcacg ctcctgatgc
    16861 aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct gtttattatg cgtcctgtcg
    16921 ccaccacgct gctgatggtg gcgatcttac tcgccgggat tatcggttat cgcgccctgc
    16981 ccgtttcggc gctgccggaa gtggactatc caaccattca ggtggtcacg ctttatccag
    17041 gtgcaagccc ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc
    17101 agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt
    17161 tacagttcca gctaacattg ccgctggatg tcgccgagca ggaagtgcag gccgcgatta
    17221 acgctgcgac caacctgcta ccgagcgatc tgcctaaccc gccggtttac agcaaagtga
    17281 acccggcaga tccaccgatc atgacgctcg ctgtcacctc aaccgccatg ccgatgacgc
    17341 aggtggaaga tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtag
    17401 ggctggtgac actttccggc gggcagcgtc cggcggtacg cgtaaaactc aacgctcagg
    17461 caattgccgc tctcggcctg accagcgaaa gtgtgcgcac cgccattact ggcgcgaacg
    17521 ttaactcagc gaaagggagc cttgacggcc cttctcgcgc ggtcacgctg tcagcgaatg
    17581 accagatgca atccgccgaa gagtatcgcc agctaatcat cgcctaccag aacggcgcac
    17641 caattcgtct tggcgatgtc gcaaccgtag agcaaggagc agaaaacagc tggctcggcg
    17701 cgtgggcgaa caaagaacag gccattgtga tgaatgttca gcgccagccc ggtgctaaca
    17761 ttatctccac cgccgacagc atccggcaga tgctaccaca gctcaccgag agtctgccga
    17821 aatcggtgaa ggtgacggta ctttctgacc gtaccaccaa tatccgcgca tcggtcgacg
    17881 atacccagtt tgagctgatg atggctatcg cgctggtagt catgattatc tacctgtttt
    17941 tgcgcaatat tccggcgacc atcattcccg gtgttgctgt accgctgtcg ttaattggca
    18001 cctttgcggt catggtgttt ctcgattttt caatcaataa cctgacgttg atggcgttaa
    18061 ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct
    18121 atatcgaaaa aggcgaaaaa ccgctggcag ccgcgctcaa aggcgcgggt gaaatcggct
    18181 ttaccattat ctcgctgacc ttctcgctga ttgcggtatt gatccccctg ctgtttatgg
    18241 gcgatatcgt cgggcgactg ttccgcgaat ttgctattac cctggcagtc gcgattttga
    18301 tctcagcagt ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg ctcagccagg
    18361 agtcgctgcg taaacagaac cgcttctccc gtgcctcgga gaaaatgttc gacaggataa
    18421 tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg accttaagcg
    18481 tagcgctcag cacgctgctg cttagcgtgc tgctgtgggt gttcattccg aaaggtttct
    18541 tcccggtaca ggacaacggc ataattcagg gcactttgca ggcaccgcaa tccagctcct
    18601 ttgccaatat ggcccagcgg caacgccagg tcgcggacgt gattttgcag gatccggcag
    18661 tgcaaagttt gacctcattt gttggcgttg atggcactaa cccgtcgctg aacagtgcgc
    18721 gtttacagat caatctcaaa ccactggatg aacgagatga ccgggtgcaa aaagtcatcg
    18781 cccgtctgca aacggcggtg gataaagtgc cgggcgtaga tctcttcctg caaccaacgc
    18841 aggacctgac tattgatact caggtcagcc gcacccagta ccagtttacc ttgcaggcca
    18901 cgtcactgga tgcgctcagt acctgggtgc cacagttgat ggaaaaactc cagcagctgc
    18961 cacagctttc tgatgtctcc agcgactggc aggacaaagg gctggtggcg tatgtcaatg
    19021 tcgacaggga cagcgccagc cgtctgggga tcagcatggc ggatgtcgat aacgccctgt
    19081 acaacgcgtt tggtcagcga ctgatttcca ctatttatac tcaggctaac cagtatcgcg
    19141 tggtgctgga acacaacacc gaaaataccc caggcctcgc ggcgctggat accattcgcc
    19201 tgaccagcag cgacggtggc gtggtgccgt taagctcaat tgccaaaatt gagcagcgtt
    19261 ttgcgccgct ttccatcaac catctggatc agttcccggt aacgactatc tcctttaacg
    19321 tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat tatggacaca gagaagacgc
    19381 tgaatctgcc ggtggatatc accacgcagt tccagggcag caccctcgcc ttccagtcgg
    19441 cgctgggcag cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg
    19501 gcattctgta cgagagcttt attcacccga tcaccattct ctcgacgcta cccaccgcag
    19561 gggtcggcgc gctgctggcg ttaatgattg ccggtagcga actggatgtg attgccatta
    19621 tcggcattat tttgctgatc ggtatcgtga agaagaacgc catcatgatg atcgacttcg
    19681 cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga agctatttac caggcctgtc
    19741 tgttgcgttt tcgtccgatc ctgatgacca ctctggcggc tctgcttggt gcgctgccgc
    19801 tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg
    19861 gcggtctgat tgtcagccag gtgctgacgc tgtttaccac gccggtgatt tatctgttgt
    19921 tcgaccgtct ggcattgtgg accaaaagcc gttttgcccg tcatgaagag gaggcgtaag
    19981 tgaagttttt tgccctcttc atttaccgcc cggtggcgac gattttactg tcggtcgcca
    20041 ttaccctgtg cggcatactg ggtttccgta tgctgccagt cgccccgctg ccgcaggtcg
    20101 attttccggt gatcatggtc agcgcctcgc tgcccggtgc gtcaccagaa acaatggcgt
    20161 cttccgttgc cacgccgctg gagcgctcac ttgggcgcat tgccggagtc agcgaaatga
    20221 cctccagcag ttcgctcggc agcacgcgca ttattttgca gtttgatttt gaccgggata
    20281 tcaacggcgc agcgcgcgat gtgcaggcgg cgatcaacgc cgcgcaaagt ctgctaccca
    20341 gcgggatgcc cagccgtccg acctatcgca aagcgaaccc atcggatgcg ccaattatga
    20401 tcctcacgct gacatccgat acttattcgc agggtgaact gtacgatttc gcctcgacac
    20461 agctggctcc gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtt ggcggcagct
    20521 cgctgcccgc cgtacgcgtc gggctgaatc cgcaggcgct gtttaatcag ggcgtctcgc
    20581 tggacgacgt acgcaccgcc atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg
    20641 aagatggtac tcaccgctgg cagatccaga ctaacgatga actgaaaacc gccgctgaat
    20701 atcagccgct gattattcat tacaacaacg gcggagcggt tcgtctgggc gatgtggcga
    20761 cggtgaccga ctcagtacag gatgtgcgca acgccgggat gaccaacgcc aaaccggcta
    20821 ttttgctgat gatccgcaag ctgccggaag ccaatattat ccagacggta gacagcatcc
    20881 gcgcccgtct gccggaatta caatccacca tcccggcagc aattgatctg caaattgccc
    20941 aagatcgctc ccctactatc cgcgcgtcgc tggaagaggt tgagcagacg ctggtaatct
    21001 cagttgcact ggtgattctg gtggtgtttt tgttcctgcg ttcgggtcgc gccaccatta
    21061 ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg tacctgtgtg
    21121 gtttcagtct caataatctt tcgttaatgg cgctcactat cgccactggc tttgtggtgg
    21181 atgacgccat cgtggtgctg gaaaacattg cccgtcatct ggaagcgggg atgaaaccgc
    21241 tacaggccgc gctacaaggt actcgcgaag tcggctttac ggtgctgtcg atgagtctgt
    21301 cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg attgccgggt cgcctgttac
    21361 gcgagtttgc cgtgacgctt tctgtcgcca ttggtatttc gctgctggtt tccctgacgt
    21421 taacgccgat gatgtgcggc tggatgctaa aagccagcaa gccgcgcgag caaaagcgac
    21481 tgcgtggctt tggtcgcatg ttggtagccc tgcaacaagg ctatggcaag tcgctgaaat
    21541 gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct tggcaccatt gcgctgaata
    21601 tctggctgta tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga
    21661 tgggtgggat acaggctgac cagagtattt cgtttcaggc gatgcgcggt aagttgcagg
    21721 acttcatgaa aattatccgt gacgatccgg cggtggataa tgtcaccggc tttaccggcg
    21781 gttcacgggt gaacagtgga atgatgttta tcacccttaa accacgcggc gagcggagcg
    21841 aaacggccca acagattatc gatcgcttgc gtaaaaaact ggctaaagaa ccgggagcga
    21901 atctgttcct gatggcggta caggatattc gcgttggcgg acgtcaggct aacgccagtt
    21961 accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc
    22021 gcaaaaaact ggcgacattg ccagagctgg ctgatgttaa ctccgatcag gaagataatg
    22081 gcgcggagat gaaccttatt tacgatcgcg acaccatggc gcgattggga attgacgtgc
    22141 aagccgccaa cagcctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc
    22201 agccgatgaa ccagtacaaa gtggtgatgg aagtggatcc gcgctatacc caggacatca
    22261 gtgcactgga aaaaatgttc gttatcaata acgaaggaaa agcgatcccg ctgtcgtatt
    22321 tcgctaaatg gcaaccggcg aacgctccac tgtcggtgaa tcatcaggga ttatcggcgg
    22381 cctcgaccat ttcgttcaac ctgccgaccg ggaaatcgct ctcggacgcc agtgcggcga
    22441 tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggaa
    22501 cagcgcaggt gttccaggag acgatgaact cacaggtgat cctgattatc gccgccatcg
    22561 ccacggtata tatcgtactg ggtatccttt acgagagtta tgtgcatcca ctgacgattc
    22621 tctccaccct accttcggcg ggcgtcggag cgctgttggc gctggagctg ttcaatgccc
    22681 cgttcagcct aattgcgctg atagggatca tgttattaat cggcatcgtg aagaaaaacg
    22741 ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg
    22801 aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgact accctggcgg
    22861 cgctgtttgg tgcgctgccg ctggtattgt cgggcggtga cggctcggag ttgcggcaac
    22921 cattggggat caccattgtc ggcggtctgg tcatgagcca gcttctgacg ctgtatacca
    22981 cgccggtggt gtatctcttt ttcgaccgtc tgcggatgcg tttttcgcgt aaacctaaac
    23041 aagcggtaac cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttgtggattg
    23101 tggctttcgg cttctttatg cagtcgctgg acaccaccat cgttaacacc gcgcttccct
    23161 caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg
    23221 tactgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc
    23281 gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt
    23341 ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggcgcgatga
    23401 tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg
    23461 cgatgacctt tgtcacgctg cccggtcagg tcgggccgct gcttggcccg gcgctcggcg
    23521 gtctgctggt ggagtacgca tcgtggcact ggatcttttt gatcaacatt ccggtgggaa
    23581 ttattggcgc gatcgccaca ctgatgttaa tgccgaacta caccatgcag acgcggcgct
    23641 ttgatctctc cggctttctg ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg
    23701 acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg
    23761 tggtagcact ggtgctttat ctgctgcacg cccaaaataa caaccgcgcc ctgttcagtc
    23821 tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta
    23881 ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct
    23941 cgccgtttca tgccggactg atgatgatcc cgatggtact cggcagcatg ggaatgaagc
    24001 gaattgtggt acaggtagtg aatcgctttg gttatcgtcg ggtactggtg gcgaccacgc
    24061 tgggcctgtc gctggtcacc ctgttgttta tgactaccgc tctgctgggc tggtactacg
    24121 ttttgccgtt cgtcctgttt ttacaaggaa tggtcaactc gacacgtttc tcctccatga
    24181 acaccctgac gctgaaagat ctcccggaca acctggcgag cagcggcaac agtctgctgt
    24241 cgatgattat gcaattgtcg atgagtattg gcgtcaccat cgccgggctg ttgctgggac
    24301 tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca
    24361 cctggcttag catggcgtct atcatcgccc ttccggcgtt catctttgcc agagtgccga
    24421 acgatacgca tcaaaatgtg gctatttcac ggcgaaaaag gagcgcgcaa tgaagttctg
    24481 gcgacccggt attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttatt
    24541 gatcacgatg cactgggcgg tacgtatcag ttttgagcgc ggctttatcg attacatcaa
    24601 gcatggtaat gaacaacggc tgcaaatgct cggcgatgcg cttggtgagc agtacgccca
    24661 gcacgggaac tggcgcttcc tgcgtaataa cgatcgcttt gtatttcaga tcctacgttc
    24721 actggagcat gataacaacg aagataagcc cggccccggt atgccgccac acggctggcg
    24781 cacacaattt tgggtggttg atcaaaacaa caaagtgctg gttggcccgc gagcaccagt
    24841 tccacccgac ggcacacggc ggcccattat ggtcaatggt gcggaagttg gcgcggtgat
    24901 cgcctcccct gttgaacgac tgacccgtaa tactgatatc aattttgaca gacaacagcg
    24961 gcaaaccagt tggctgattg tggctttatc taccttatta gcggcgctgg cgacattccc
    25021 actggcgcgc ggtttgctgg ctccggtaaa acgactggtg gatggcacgc acaaactggc
    25081 agcgggtgat ttcactactc gcgtagcccc caccagcgaa gatgagttgg gcagactggc
    25141 agaggatttc aatcaactcg ccagcacact ggagaaaaac cagcagatgc gccgcgattt
    25201 tatggccgat atttctcatg aactgcgcac gcctttagca gtactgcgcg gcgaactgga
    25261 agccattcag gatggtgtgc gtaaattcac gccggagacg ctggcttctt tacaggcaga
    25321 agtaggtaca ctgacaaaac tggttgacga tctccatcaa ttgtctatgt ctgatgaagg
    25381 cgctctcgcc tatcaaaaag caccgataga tttgatccca ctgctggaag tggcgggagg
    25441 cgcatttcgc gaacgattcg ccagccgtgg cctgaaattg caattttccc tgccagacag
    25501 tattaccgta tttggcgatc gcgatcgttt aatgcagttg ttcaataact tactggaaaa
    25561 cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa
    25621 aacggtgcgc ctgacctttg ccgacagtgc gccgggtgtc agtgacgatc agctacaaaa
    25681 attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gtggttccgg
    25741 gctggggctg gcgatttgcc tgaacattgt tgaagcacat aacggccgca ttattgccgc
    25801 ccattcgcct tttggcgggc ttagcattac agtagagtta ccgctggaac gggatttaca
    25861 gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa
    25921 gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgcca
    25981 acgttgatta gccacggcga tcaggtactg ccatatgtgc gccagacacc accggatctg
    26041 atcctgttgg atctgatgct ccctggcacc gatggcctga cgctgtgccg ggaaattcgt
    26101 cgtttttctg acattccgat cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg
    26161 ctggggttgg agattggcgc agatgactat atctgcaagc cgtacagccc acgggaagtg
    26221 gtagcgcgcg tcaaaaccat tttgcgccgt tgcaaaccgc agcgtgagtt gcagcaacag
    26281 gatgctgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcctg gcgcggtaaa
    26341 atgcttgacc tgacgcctgc agaatttcga ctgctgaaaa cgctctctca tgaaccagga
    26401 aaagtgttct cccgcgagca gttgctgaac catctttatg acgactaccg cgtggtaacc
    26461 gaccgtacca tcgacagcca cattaaaaac ctgcgccgca agctggaatc tctcgacgcc
    26521 gaacagtcat ttatccgcgc cgtttacggc gtcggttacc gctgggaagc cgacgcctgc
    26581 cgcatcgttt aacattttta cctgccggat ctggccttca gaccagatcc ggcaacatta
    26641 tcccacgcat agtcagcaaa ctgacatgat ctctccgccc tggcaggcac cagaaaatcg
    26701 ctcatacttt aatcggcaaa cagtcctttt agatgctgtt ttcgatacgc aatttcaatc
    26761 aaggagtcat tatggctggt tggtttgaac tcagtaagag cagtgataat caattccggt
    26821 ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag cgagctgtat acctcaaaag
    26881 cctctgcgga aaagggcatc gcgtcggtac gtagcaacag cccgcaagaa gaacgctatg
    26941 agaaaaaaac ggcaagcaac ggcaaattct atttcaatct gaaagccgcc aatcatcaaa
    27001 ttatcggctc cagccagatg tacgccaccg cgcaatctcg tgaaaccgga attgcctccg
    27061 ttaaagcaaa tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt
    27121 aggttgttgg cgtccggcgc tttaccagtt acctccacag cgctacaatg cccgccctta
    27181 aagtgggggc actcccctga ccgcttcatc aggtgaagcg gatctgacct gtcatcagaa
    27241 cgagagaatt atgtttaaac cggaactcct ttccccggcg ggaacgctga aaaatatgcg
    27301 ttacgctttc gcttatggcg cagatgctgt ttatgcgggc cagccgcgtt actccctgcg
    27361 tgtgcgcaac aacgaattca accacgaaaa tcttcaactc ggcattaacg aagcccacgc
    27421 gctggggaaa aagttttatg tcgtggtcaa cattgcaccg cacaacgcca agctgaaaac
    27481 ctttatccgt gacctgaaac cggtggtgga aatggggccg gatgcgctga ttatgtccga
    27541 tccagggctg attatgctgg tgcgtgagca cttccctgaa atgccgatcc atctctcggt
    27601 acaggctaac gccgtgaact gggcgacggt gaaattctgg cagcaaatgg gcctgacccg
    27661 cgtgatcctc tctcgcgaac tgtcactgga agagattgaa gagatccgca atcaggtgcc
    27721 ggatatggaa atcgaaatct tcgttcacgg cgcgctgtgc atggcctact ccggtcgctg
    27781 cctgctctct ggctatatca acaagcgcga cccgaaccag ggcacctgca ccaacgcctg
    27841 ccgctgggag tacaacgtcc aggaagggaa agaagatgac gttggcaaca tcgtacacaa
    27901 gtacgagccg attccggtgc aaaatgttga gccgacgctg ggtatcggcg cgccaaccga
    27961 caaagtgttt atgatcgaag aagcccagcg tccgggcgag tatatgaccg cgtttgaaga
    28021 tgagcacggc acttacatca tgaactcgaa agatctgcgc gccatcgccc atgttgaacg
    28081 cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggc cgtaccaaat ctttctacta
    28141 ttgcgcacgc accgcgcagg tttaccgtaa agctatcgat gacgccgctg cgggcaaacc
    28201 gttcgatacc agcctgctgg aaactctgga aggtctggcg catcgtggct ataccgaagg
    28261 tttcctgcgt cgtcatactc acgacgatta tcagaactac gaatacggtt attcagtttc
    28321 tgaccgccag cagtttgttg gtgagtttac cggtgagcgc aagggggacc tcgcggcggt
    28381 agcggtgaaa aataaattct ccgttggcga cagccttgag ctaatgacgc cgcaaggcaa
    28441 cattaacttt acccttgagc acatggaaaa cgccaaaggt gaagctatgc cggtcgcacc
    28501 aggcgatggt tatactgtgt ggctcccggt gccgcaggat cttgagctaa attacgcgct
    28561 gctgatgcgt aatttctccg gggaaaccac gcgtaacccc cacggtaagt gattaatttc
    28621 gattattttt cccggatgga aaattcttag aaaccgatca catacagctg catttattaa
    28681 ggttatcatc cgtttcgctg aaaaacataa cccataaaat gctagctgta ccaggaacca
    28741 cctccttagc ctgtgtaatc tcccttacac aggcttattt tttatgcata agccctatcc
    28801 ctggtcaccg tcttccattg accacatcga tagaatctcc cttaatagca cgatgccttt
    28861 cacttatcga catcgtgctc gcacaggttc cggttacgca cagccagaac gcgcatgttt
    28921 gacgcttacc aaaaaatgaa cctaaagcat tggaatattt ttgacatcat tttctgatgg
    28981 ctgcataaaa taaaaattct gctttagttt catctatctg tttgtcatta ttactcacat
    29041 tcaataatgg tgttgaagaa tatcccatca cagataaaat aaatatgtgc atggtagtct
    29101 tgaatctatt ctcactctcc acatttgaat gtcagacgag caacgccatg taatcctgca
    29161 ccttctgtct tcaatttaac tatctgcatt tttttgccct gagtaacaca gaaatgggct
    29221 gcatcatttt ttactatatt ttctgcacca gatattctgc ccctggctaa agaagcttcg
    29281 gcttcggtgt agtattggtt atcgagttta cgctgaatat tacttttata tgcaagacca
    29341 aatttaccga tacttgtctc atcattatgt acagcacacc cagacataag aaaaatac
//
LOCUS       DACADA010000046        29224 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.046, whole genome shotgun sequence.
ACCESSION   DACADA010000046 DACADA010000000
VERSION     DACADA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.046"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(173..1648)
                     /gene="ampG"
                     /locus_tag="ISB17_002001"
     CDS             complement(173..1648)
                     /gene="ampG"
                     /locus_tag="ISB17_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAP1979935.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1692..2270)
                     /locus_tag="ISB17_002002"
     CDS             complement(1692..2270)
                     /locus_tag="ISB17_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1979936.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2575..2892
                     /gene="bolA"
                     /locus_tag="ISB17_002003"
     CDS             2575..2892
                     /gene="bolA"
                     /locus_tag="ISB17_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAP1979937.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3236..4534
                     /gene="tig"
                     /locus_tag="ISB17_002004"
     CDS             3236..4534
                     /gene="tig"
                     /locus_tag="ISB17_002004"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP1979938.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4780..5403
                     /gene="clpP"
                     /locus_tag="ISB17_002005"
     CDS             4780..5403
                     /gene="clpP"
                     /locus_tag="ISB17_002005"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP1979939.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5529..6803
                     /gene="clpX"
                     /locus_tag="ISB17_002006"
     CDS             5529..6803
                     /gene="clpX"
                     /locus_tag="ISB17_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAP1979940.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6991..9345
                     /gene="lon"
                     /locus_tag="ISB17_002007"
     CDS             6991..9345
                     /gene="lon"
                     /locus_tag="ISB17_002007"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAP1979941.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9554..9826
                     /gene="hupB"
                     /locus_tag="ISB17_002008"
     CDS             9554..9826
                     /gene="hupB"
                     /locus_tag="ISB17_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAP1979942.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10018..11889
                     /gene="ppiD"
                     /locus_tag="ISB17_002009"
     CDS             10018..11889
                     /gene="ppiD"
                     /locus_tag="ISB17_002009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP1979943.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12040..12411
                     /locus_tag="ISB17_002010"
     CDS             12040..12411
                     /locus_tag="ISB17_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979944.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            12529..12927
                     /locus_tag="ISB17_002011"
     CDS             12529..12927
                     /locus_tag="ISB17_002011"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAP1979945.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12979..13674)
                     /gene="queC"
                     /locus_tag="ISB17_002012"
     CDS             complement(12979..13674)
                     /gene="queC"
                     /locus_tag="ISB17_002012"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAP1979946.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13739..15439)
                     /locus_tag="ISB17_002013"
     CDS             complement(13739..15439)
                     /locus_tag="ISB17_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAP1979947.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15539..16357
                     /gene="cof"
                     /locus_tag="ISB17_002014"
     CDS             15539..16357
                     /gene="cof"
                     /locus_tag="ISB17_002014"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAP1979948.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16510..16968
                     /locus_tag="ISB17_002015"
     CDS             16510..16968
                     /locus_tag="ISB17_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP1979949.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16998..18770
                     /locus_tag="ISB17_002016"
     CDS             16998..18770
                     /locus_tag="ISB17_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP1979950.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18763..20544
                     /locus_tag="ISB17_002017"
     CDS             18763..20544
                     /locus_tag="ISB17_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP1979951.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     SLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20725..21063
                     /gene="glnK"
                     /locus_tag="ISB17_002018"
     CDS             20725..21063
                     /gene="glnK"
                     /locus_tag="ISB17_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP1979952.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21093..22379
                     /gene="amtB"
                     /locus_tag="ISB17_002019"
     CDS             21093..22379
                     /gene="amtB"
                     /locus_tag="ISB17_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAP1979953.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTAKEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22428..23288)
                     /gene="tesB"
                     /locus_tag="ISB17_002020"
     CDS             complement(22428..23288)
                     /gene="tesB"
                     /locus_tag="ISB17_002020"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAP1979954.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23506..24078
                     /locus_tag="ISB17_002021"
     CDS             23506..24078
                     /locus_tag="ISB17_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979955.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24109..24420)
                     /locus_tag="ISB17_002022"
     CDS             complement(24109..24420)
                     /locus_tag="ISB17_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAP1979956.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24583..24679
                     /gene="ffs"
                     /locus_tag="ISB17_002023"
     ncRNA           24583..24679
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ISB17_002023"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24799..25152
                     /locus_tag="ISB17_002024"
     CDS             24799..25152
                     /locus_tag="ISB17_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HAP1979957.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DIPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25194..26744)
                     /gene="pdeB"
                     /locus_tag="ISB17_002025"
     CDS             complement(25194..26744)
                     /gene="pdeB"
                     /locus_tag="ISB17_002025"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAP1979958.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWYRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26908..27378)
                     /locus_tag="ISB17_002026"
     CDS             complement(26908..27378)
                     /locus_tag="ISB17_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979959.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27494..28045)
                     /gene="maa"
                     /locus_tag="ISB17_002027"
     CDS             complement(27494..28045)
                     /gene="maa"
                     /locus_tag="ISB17_002027"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAP1979960.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28219..28437)
                     /gene="hha"
                     /locus_tag="ISB17_002028"
     CDS             complement(28219..28437)
                     /gene="hha"
                     /locus_tag="ISB17_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAP1979961.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28463..28837)
                     /gene="tomB"
                     /locus_tag="ISB17_002029"
     CDS             complement(28463..28837)
                     /gene="tomB"
                     /locus_tag="ISB17_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAP1979962.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7384 a         6993 c         7864 g         6983 t
ORIGIN      
        1 aaataaggac tcatcagctg aatcgtgtaa aaaaaataga attcataaat ggcgtcaata
       61 atttccagac gctaagccgc acaaaagaac aaatatcaat aagcgatgct gatgtttatt
      121 tagccgtaat aattacggcg agtgattact acagctaaat aatatttaca gattacgtca
      181 gatgtgtttt tcgtagcgcc agataatcca gcaaaccacc aagcacgacg ccagagaggg
      241 cgactaaaac cccgacttcc agcagcgcag gcaggaaaga gaagtgcgtc aaatccagcg
      301 cgtccatcgt caacagcagt aaccacacgg ccaacaggct gatgcccgcc gccagtgtcc
      361 acatggcaaa ggcataacct gcaggatatt cggtacggga gataaagttg tcatttactc
      421 gtgtatattc aagcgtctgg cggcaaacca gcagcaaaat aagccctggt acggcagcgg
      481 cgacggagaa tagatagaat gtcgaccagc cgtgtgcttc aacaaaccaa cccgccacgg
      541 ggccgacata aactcgccct acagcagaaa gcgctgagag cagggcaaat tgagtagcgg
      601 aaaatgactt attacacagc gtcattaaca gcgcgacaaa ggctgatgta cccatcccgc
      661 cacagaggtt ttcgaaaaag acggctgcac ccatgctgta gagatgctta tcagtaatcg
      721 acagcagcca gtaaccggcg ttagatgcac cttgtaaaat gccgaaaatc agcagtgcac
      781 ggaacagcga caggcgctgc atcaaaatcc caccgtacaa tgcgccaaca atggtcgcca
      841 gtaagccaag cgttttgtta accacgccta cttcacccgc atcaaacccg acgccgcgaa
      901 tcaaaaacgt ggttgtcagg ctcatagcga atgcgtcgcc cagcttatac agcacgataa
      961 gaagcaaaat aagccaggca ttattgcgac caaagaaatc tcgcagaggt gcaacaaccg
     1021 cttgttccag cgttttgggc acaggaatgg tgtcggtggg ttctggtgca agcaacgtcg
     1081 cgataataca ggggatcaac agcgccgcca tcaaccagta catgccctgc cagcccagcc
     1141 atttatctgc cagccacagg gctaagccgc cggaaaccag catccctaaa cggtaaccca
     1201 gcacgctgat tgccgcgccc gcaccacgtt cttctgccgg gagcacatcg gttttccacg
     1261 catcgaagac aatatcctgg gaggcagagc aaaaagcgat caccaccgcc agtgccgcca
     1321 tccagcggag ctgggtgcct ggttcgagaa aacccatcgc cgcaatggcg actaataaca
     1381 gaatttgcgt ggcgagcagc caaccgcgcc gccgcccgaa aaatgggggc gtgtagcggt
     1441 ccatcagtgg tgaccagagg aatttaaaaa cgtaagcctg gcctaccaga gagaagaaac
     1501 caatggtttt gagatcgata ttctcgaccg tcatccaggc ctgtaaggtg ccggacgtca
     1561 gggcgagcgg tagcccggaa gcaaaaccca ggatcagcaa tatggctgaa cgcggctgtt
     1621 gaaaaatacg taaatattga ctggacatgg gcgtctacag gcccggcttg caccgggcca
     1681 gagggcagaa attaacgcgc gttctgcttg atgaattcgt ggatgctggt gtcctgagac
     1741 atatcagcga tggtatcggt cagcacactg ttaaccgcat cggcgatatt tttgttggag
     1801 gcctggaacg caccttcaac gttgtagctg gcacgatagt ttttggtcat tttgttgcca
     1861 ttctgcgcgg tagcgatgat ggcgatatcc gctttggtcg cgatgttgta gcgcacgttg
     1921 ccctgggaca cgtcagcata cagttggcta acgatgattt gcagattaac cgggccattc
     1981 ggaccaacca tgtatccacg ggcggtcatt tgtttttcca gcacttcttg cagcaggaaa
     2041 cgcagatcgc gggaggcggt cagggtaacg atttgattat cgcgggtgac ttttgccagc
     2101 gcctgatcgg tacgctgatc ggcaccatta atgcttacgg tgacgcccat caggcttgga
     2161 tcctgctgtg gcagtgtaat cgtcggggaa acttcgatag ttgttggcgg ttttgcgcat
     2221 cctgcaagca taaacagagc aactaacggg aagaggattt ttttgaacat gttcgggctc
     2281 tcagagactc ttaagcgtgt ttggtaaaaa ttcccgccat cataacattg ccaacggcga
     2341 ggggaagtgg gtaaggcatg taaattcatc atgttgacga aataatcacc cctggtaaaa
     2401 gaaacgctga tgcgaggcct gtgtttcaat ctttaaatca gtaaacttca tacgcttgac
     2461 ggaaaaacca ggacgaaacc taaatatttg ttgttaagct gcaatggaaa cggtaaaagc
     2521 ggctagtatt taaagggatg gatgacatct cagcgttgtc ggaggagata tttcatgatg
     2581 atacgtgagc ggatagaaga aaaattaagg gcggcgttcc aacccgtatt cctcgaagta
     2641 gtggatgaaa gctatcgtca caatgtccca gccggctctg aaagccattt taaagttgtg
     2701 ctggtcagcg atcgttttac gggtgaacgt tttctgaatc gtcatcgaat gatttacagt
     2761 actttagcgg aggaactctc tactaccgtt catgcgctgg ctctgcatac ttacactatt
     2821 aaggagtggg aagggttgca ggacaccgtc tttgcctctc ctccctgtcg tggagcagga
     2881 agcatcgcgt aaaaacgcat ttgcaactgt cggcgctttt ccagtatgtt gctaaagatt
     2941 ttatgaaaaa cggcctgcgg gccgttttgt tttgtctgga ttttgcgctt tttgcccagc
     3001 attcagacga aaatcgcccg ggaattgtga aaaaatacgc gacagcgcgc aataaccgtt
     3061 ctcgactcat aaaagtgatg ccgctataat gccgcgtcct atttgaatgc tttcgggatg
     3121 attctggtaa cagggaatgt gattgattat aagaacatcc cggttccgcg aagccaacaa
     3181 cctgtgcttg cggggtaaga gttgaccgag cactgtgatt ttttgaggta acaagatgca
     3241 agtttcagtt gaaaccactc aaggccttgg ccgccgtgta acgattacta tcgctgctga
     3301 cagcatcgag accgctgtta aaagcgagct ggtcaacgtt gcgaaaaaag tacgtattga
     3361 cggcttccgc aaaggcaaag tgccaatgaa tatcgttgct cagcgttatg gcgcgtctgt
     3421 acgccaggac gtcctgggcg acctgatgag ccgtaacttc attgacgcca tcattaaaga
     3481 aaaaatcaat ccggctggcg caccgaccta tgttccgggc gaatacaagc tgggtgaaga
     3541 cttcacctac tctgtagagt ttgaagttta tccggaagtt gaactgcagg gtctggaagc
     3601 gatcgaagtt gaaaaaccga ttgttgaagt gaccgacgct gacgttgacg gcatgctgga
     3661 tactctgcgt aaacagcagg caacctggaa agaaaaagac ggcgctgttg aagcagaaga
     3721 ccgtgtgacc atcgacttca ccggttctgt agacggagaa gagttcgaag gcggtaaagc
     3781 gtctgatttc gtactggcga tgggtcaggg tcgcatgatc ccgggctttg aagacggtat
     3841 caaaggccac aaagctggcg aagagttcac catcgacgtg accttcccgg aagagtacca
     3901 cgcagaaaac ctgaaaggta aagcagcgaa attcgctatc aacctgaaga aagttgaaga
     3961 gcgtgaactg ccggaactga ctgaagaatt catcaaacgt ttcggcgttg aagatggttc
     4021 cgtagaaggt ctgcgcgctg aagtgcgtaa aaacatggag cgcgagctga agagcgccat
     4081 ccgtaaccgc gttaagtctc aggcgatcga aggtctggta aaagctaacg acatcgatgt
     4141 accggctgcg ctgatcgaca gcgaaatcga cgttctgcgt cgccaggctg cacagcgttt
     4201 cggtggcaac gaaaaacaag ctctggaact gccgcgcgaa ctgttcgaag aacaggctaa
     4261 acgccgcgta gttgttggcc tgctgctggg cgaagttatc cgcaccaacg agctgaaagc
     4321 tgacgaagag cgcgtgaaag gcttgatcga agagatggct tctgcgtacg aagatccgaa
     4381 agaagttatc gagttctaca gcaaaaacaa agaactgatg gacaacatgc gcaatgttgc
     4441 tctggaagaa caggctgttg aagctgtact ggcgaaagcg aaagtgactg aaaaagaaac
     4501 cactttcaac gagctgatga accagcaggc gtaatttacg tagcataacg cgctaaattc
     4561 gcacaaaggc ccgtcaccgc caggtggtgg gctttttttt gtcatgaatt ttgcatggaa
     4621 ccgtgcgaaa agcctctttc ggtgttagcg taacaacaaa agattgttat gcttgaaata
     4681 tggtgatgcc gtacccataa cacagggact agctgataat ccgtccataa ggttacaatc
     4741 ggtacagcag gttttttcaa ttttatccag gagacggaaa tgtcatacag cggcgaacga
     4801 gataactttg caccccatat ggcgctggtg ccgatggtca ttgaacagac ctcacgaggt
     4861 gagcgctctt ttgatatcta ttctcgtcta cttaaggaac gcgtcatttt tctgactggc
     4921 caggttgaag accacatggc taacctgatt gtggcgcaga tgctgttcct ggaagcagaa
     4981 aacccagaaa aagatatcta tctgtacatt aactctccag gcggggtgat tactgccggg
     5041 atgtctatct atgacaccat gcagtttatc aagcctgatg tcagcaccat ctgtatgggc
     5101 caggcggcct cgatgggcgc tttcttgctg actgcagggg caaaaggtaa acgtttctgc
     5161 ctgccgaatt cgcgcgtgat gattcaccag ccgttgggcg gctaccaggg ccaggcgacc
     5221 gatatcgaaa ttcatgcccg tgaaattctg aaagttaaag ggcgcatgaa tgaacttatg
     5281 gcgcttcata cgggtcaatc attagaacag attgaacgtg ataccgagcg cgatcgcttc
     5341 ctttctgccc ctgaagcggt ggaatacggt ctggtcgatt cgattctgac ccatcgtaat
     5401 tgatgccaga ggcgcaactg tgccgctata cttatccagg gcggcacaac gctgtaagcg
     5461 gcttgcgcct gagaatggca tttgcgtcgt cgtgtgcggc acaaagaaca aagaagaggt
     5521 tttgacccat gacagataaa cgcaaagatg gctcaggcaa attgctgtat tgctcttttt
     5581 gcggcaaaag ccagcatgaa gtgcgcaagc tgattgccgg tccatccgtg tatatctgcg
     5641 acgaatgtgt tgatttatgt aacgacatca ttcgcgaaga gattaaagaa gttgcaccgc
     5701 atcgtgaacg cagtgcgcta ccgacgccgc atgaaattcg caaccacctg gacgattacg
     5761 ttatcggtca ggaacaggcg aaaaaagtgc tggcggtcgc ggtatacaac cactacaaac
     5821 gtctgcgcaa cggagatacc agcaatggcg tcgagttggg caaaagtaac attctgctga
     5881 tcggtccgac cggttccggt aaaacgctgc tggccgaaac gctggcgcgc ctgctggacg
     5941 ttccgttcac catggctgac gcaaccacgc tgaccgaagc cggttatgtg ggcgaagacg
     6001 ttgaaaacat cattcagaag ctgttgcaga agtgcgatta cgacgtacag aaagcgcagc
     6061 gcgggattgt ctacatcgat gaaatcgaca agatttctcg taagtcagac aacccgtcca
     6121 ttacccgaga cgtttccggt gaaggcgtac agcaggcact gttaaaactg atcgaaggta
     6181 cggtagctgc tgttccgccg caaggtgggc gtaaacatcc gcagcaggaa ttcttgcagg
     6241 ttgatacctc taagatcctg ttcatctgtg gcggcgcatt tgctggcctg gataaagtga
     6301 ttgccaatcg tgttgaaacc ggctccggca ttggttttgg cgcgacggtg aaagccaagt
     6361 ccgacaaagc cagcgaaggt gaactgctgg cgcaggttga accggaagat ctgatcaagt
     6421 ttggtctgat ccctgagttc attggtcgtc tgccggttgt cgcaacgttg aatgaactga
     6481 gcgaagaagc tctgattcag atcctcaaag agccgaaaaa cgccctgacc aagcagtatc
     6541 aggcgctgtt taatctggaa ggcgtggatc tggaattccg tgacgaggcg ctggatgcta
     6601 tcgctaagaa agcgatggcg cgtaaaaccg gtgcccgtgg cctgcgttcc atcgtagaag
     6661 ccgcactgct cgataccatg tacgatctgc cgtccatgga agatgtcgaa aaagtggtta
     6721 tcgacgagtc ggtaattgat ggtcaaagca aaccgttgct gatttatggt aagccggaag
     6781 cgcaacaggc atctggtgaa taattaacca ttcccataca attagttaac cgaaaagggg
     6841 ggattttatc tcccctttaa tttttcctct attctcggcg ttgaatgtgg gggaaacatc
     6901 cccatatact gacgtacatg ttaatagatg gcgtgaagca cagtcgtgtc atctgattac
     6961 ctggcggaaa ttaaactaag agagagctct atgaatcctg agcgttctga acgcattgaa
     7021 atccccgtat tgccgctgcg cgatgtggtg gtttatccgc acatggtcat ccccttattt
     7081 gtcgggcggg aaaaatccat ccgttgtctg gaagcggcga tggaccatga taaaaaaatt
     7141 atgctggtcg cgcagaaaga agcttcaacg gatgagccgg gtgtaaacga tcttttcacc
     7201 gtcgggaccg tggcctctat attgcagatg ctgaaactgc ctgacggcac cgtcaaagtg
     7261 ctggtcgagg ggttacagcg cgcgcgtatt tctgcgctct ctgacaatgg cgaacacttt
     7321 tctgcgaagg cggagtatct ggagtcgccg accattgatg agcgggaaca ggaagtgctg
     7381 gtgcgtactg caatcagcca gttcgaaggc tacatcaagc taaacaaaaa aatcccacca
     7441 gaagtgctga cgtcgctgaa tagcatcgac gatccggcgc gtctggcgga taccattgct
     7501 gcacatatgc cgctgaaact ggctgacaaa cagtccgttc tggagatgtc cgacgttaac
     7561 gaacgtctgg aatatctgat ggcaatgatg gaatcggaaa tcgatctgct gcaggttgag
     7621 aaacgcattc gcaaccgcgt taaaaagcag atggagaaat cccagcgtga gtactatctg
     7681 aacgagcaaa tgaaagctat tcagaaagaa ctcggtgaaa tggacgacgc gccggacgaa
     7741 aacgaagccc tgaagcgcaa aatcgacgcg gcgaagatgc cgaaagaggc aaaagagaaa
     7801 gcggaagcag agctgcagaa gctgaaaatg atgtctccga tgtcggcaga agcgaccgta
     7861 gtgcgtggat atatcgactg gatggtacag gtgccgtgga atgcgcgtag caaggtcaaa
     7921 aaagacctgc gtcaggcgca ggaaatcctt gataccgacc attatggtct ggagcgcgtg
     7981 aaagatcgta tccttgagta tcttgcggtt caaagccgtg tcaacaaaat caagggtccg
     8041 atcctctgcc tggtagggcc gccgggggta ggtaaaacgt ccctgggtca gtccatcgcc
     8101 aaagccaccg ggcgtaaata tgtccgtatg gcgctgggcg gcgtgcgtga tgaagcggaa
     8161 atccgtggtc accgccgtac ttacatcggt tctatgccgg gtaaactgat ccagaaaatg
     8221 gcgaaagtgg gcgtgaaaaa cccgctgttc ctgctcgatg agatcgacaa aatgtcttct
     8281 gacatgcgtg gtgatccggc ctccgcactg cttgaagtgc tggatccaga gcagaacgta
     8341 gcgttcagcg accactacct ggaagtggat tacgatctca gcgacgtaat gtttgtcgct
     8401 acgtcgaact ccatgaacat tccggcaccg ttgctggatc gtatggaagt gattcgcctc
     8461 tccggttata ccgaagatga aaaactgaac atcgccaaac gtcacctgct gccgaagcag
     8521 attgaacgta atgcattgaa aaaaggtgag ctgaccgtcg acgatagcgc cattatcggc
     8581 attattcgtt actacacccg tgaggcgggc gtgcgtggtc tggagcgtga aatctccaaa
     8641 ctgtgtcgca aagcggttaa gcagttactg ctcgataagt cattaaaaca tatcgaaatt
     8701 aacggtgata acctgcatga ctacctcggt gttcagcgtt tcgactatgg gcgcgcggat
     8761 aacgaaaacc gtgtcggcca ggtaaccggt ctggcgtgga cggaagtggg cggtgacttg
     8821 ctgaccattg aaaccgcgtg cgttccgggg aaaggcaaac tgacctatac cggttcgctc
     8881 ggcgaagtga tgcaggagtc tattcaggcg gcgttaacgg tggttcgtgc gcgtgcggaa
     8941 aaactgggga tcaaccctga tttttacgaa aaacgtgaca tccacgtcca cgtaccggaa
     9001 ggtgcgacgc cgaaagatgg tccgagtgcc ggtattgcca tgtgcaccgc gctggtttct
     9061 tgcctgaccg gtaacccggt tcgtgccgat gtggcaatga ccggtgagat cactctgcgt
     9121 ggtcaggtac tgccgatcgg tggtttgaaa gaaaaactac tggcagcgca tcgcggcggg
     9181 attaaaacag tgttaattcc gttcgaaaat aaacgcgatc tggaagagat tcctgacaac
     9241 gtaattgccg atctggacat tcatcctgtg aagcgcattg aggaagttct gactctggcg
     9301 ctacaaaatg aaccgtctgg catgcaggtt gtgactgcaa aatagtgacc tcgcgcaaaa
     9361 tgcactaata aaaacagggc tggcaggcta attcgggctt gccagccttt ttttgtctcg
     9421 ctaagttaga tggcggatcg ggcttgccct tattaagggg tgttgtaagg ggatggctgg
     9481 cctgatataa ctgctgcgcg ttcgtacctt gaaggattca agtgcgatat aaattataaa
     9541 gaggaagaga agagtgaata aatctcaatt gatcgacaag attgctgcag gggctgatat
     9601 ctctaaagct gcggctggcc gtgcgttaga tgctattatt gcttccgtaa ctgaatctct
     9661 gaaagaaggg gatgatgtag cactggtagg ttttggtact tttgccgtta aggaacgtgc
     9721 tgcccgtact ggccgcaacc cgcagaccgg taaagagatc accatcgctg ctgctaaagt
     9781 accgagcttc cgtgcaggta aagcactgaa agacgcggta aactaagcgt tgtccccagt
     9841 ggggatgtga cgaagttcaa gggcgcatct actgatgtgc cttttttatt tgtattcggt
     9901 gactttctgc gtcttgtggg ctgacaattg cccccgtttc ttgtcacaat aggcctttgc
     9961 gcgcatcgat acgttgcgtg aggtacacag tcatctacag cggagtgttg ttacaccatg
    10021 atggacagct tacgcacggc tgcaaacagt ctcgtgctca agattatttt cggtatcatt
    10081 atcgtgtcgt tcatattgac cggcgtgagt ggttacctga ttggcggagg caataactac
    10141 gccgcaaaag tgaatgacca ggaaatcagc cgtgggcaat tcgagaacgc cttcaacagc
    10201 gagcgtaatc gcatgcagca acagctgggc gatcaatact ccgagctggc agcgaacgaa
    10261 ggctatatga aaaccctgcg tcaacaggtg ctgaatcgtc tgatcgacga ggcgcttctg
    10321 gatcagtacg ctcgtgagct gaaactgggt atcagcgatg agcaggttaa acaggcgatt
    10381 ttcgcgaccc cagccttcca ggttgacggc aaatttgata acagccgcta taacggtatc
    10441 ctcaaccaga tggggatgac cgccgatcag tacgcccagg cgctgcgtaa ccagctcact
    10501 acccaacagc tgattaacgg cgttgccggt accgatttta tgctgaaagg tgaaaccgac
    10561 gagctggcgg cactggtagc tcaacaacgc gtggtgcgtg aagcggctat cgatgttaac
    10621 gcgctggcgg cgaagcagcc tgtgaccgaa caggaaattg ccagctacta cgaacaaaac
    10681 aaaaacaatt tcatgacgcc ggaacaattc cgcgtgagtt acatcaagct ggatgccgcg
    10741 acgatgcagc aaccggttag cgatgcggat atccagagct actacgatca gcatcaggat
    10801 caattcaccc agccgcagcg tacccgttac agcatcatcc agaccaaaac tgaagatgaa
    10861 gcgaaagcgg tacttgatga gctgaataag ggcggtgatt ttgctgcatt agccaaagaa
    10921 aaatctgccg atattatctc tgcccgtaac ggcggtgata tgggttggtt agaagatgcc
    10981 actatcccgg acgaactgaa aaatgcgggt ctgaaagaaa aaggccaact gtctggtgtt
    11041 atcaaatctt cggtcggttt cctgattgta cgtctggacg acattcagcc agcgaaagtg
    11101 aaatcgttag acgaagtacg tgacgacatt gcggcgaaag tgaaacacga aaaagccctc
    11161 gatgcgtact acgcgctgca gcagaaagtg agcgatgcgg caagcaacga caccgagtct
    11221 ctggccggtg cagagcaagc tgccggcgtt aaagccactc agacgggttg gttcagcaaa
    11281 gataacctgc cggaagagtt gaacttcaag ccagttgccg acgctatctt caacggcggt
    11341 ctggtaggtg aaaacggcgc gccgggcatc aactctgaca tcatcaccgt agacggcgac
    11401 cgcgcattcg tgctgcgcat cagcgagcac aaaccggaag cggtgaaacc gttggcagat
    11461 gttcaggaac aagttaaggc actggttcag cacaacaaag ctgaacaaca ggcgaaagtg
    11521 gatgctgaga aactgctggt tgatttgaaa gccggcaaag gtgcggaagc gatgcaggct
    11581 gccggtctga aatttggcga gccgaaaacc ttaagccgtt ccggtcgtga cccgattagc
    11641 caggctgcgt ttgcactgcc gctgccagcg aaagacaaac cgagctacgg tatggcgacc
    11701 gatatgcaag gtaatgtggt tctgctggcg ctggatgaag tgaaacaagg ttcaatgccg
    11761 gaagatcaga aaaaagcgat ggtgcagggt atcacccaga acaacgcaca aatcgtcttt
    11821 gaagctctga tgagtaacct gcgtaaagag gcgaaaatca aaattggcga tgcgctggaa
    11881 cagcaataat cctgaagccg cctcgcaaaa aaatgctttg cagctgtaac aagaaaaggt
    11941 cgctttcgcg gccttttcca tttctgaaca ttgccatttg tttactgttt tcactgccgt
    12001 taaggtgatt ccactgttaa caaacaagga gaaaacagta tgaaacacgg aattaaagca
    12061 ctgctcatta ccctgtccct ggcctgtgcc ggaatgtctc atagtgctct ggcggcagct
    12121 cctgcggcaa aaccgacaac ggtagaaacc aaagcggaag ctcctgcagc acaaagtaaa
    12181 gcagcagtac cggcgaaagc cagtgacgaa gaaggcaccc gggtcagcat taataatgcc
    12241 agcgcggaag agttagcccg cgcgatgaat ggcgttggcc tgaagaaagc gcaggcgatt
    12301 gtcagttatc gcgaagagta cggtccgttt aaaacggtgg aggatctaaa gcaggtgccg
    12361 gggatgggca attcgctggt ggaacgtaat atggcggtat taaccctgta attaatttgc
    12421 atagtggcaa ttttttgcca gactgaagag gtcataccag ttatgacctc tgtacttata
    12481 acagttctta taacagttct tataacaaca acgtaaggtt attgcgctat gcaaacacaa
    12541 atcaaagttc gtggatatca tctcgacgtt taccagcacg taaacaacgc ccgctacctt
    12601 gaatttctcg aagaagcccg ctggcatggg ttggaaaata gcgacagttt tcactggatg
    12661 acggcccata acatcgcttt cgtcgtggtc aatatcaata ttaactatcg ccgcccggcg
    12721 gtattaagcg acctgttaac cattaccagt cagttgcagc aattaaacgg taaaagtggc
    12781 atcttaagcc aggtcattac actggagccg gaaggacagg tggtagcgga tgcgcttatt
    12841 acgtttgttt gtattgatct taaaacgcag aaagcattag ctctggaagg ggaattgcgc
    12901 gaaaagctgg agcagatggt taagtaaacg ttttgtggtg ccggatgctc aagccgcatc
    12961 cggcgacacc cggaataatt acttcaaccc ggttttctgc ttcatcgctg ccatcaccgt
    13021 cggtttatcg gccagataat gattcaaacc gttggcacgt aaattacacg ccgcacaatg
    13081 accgcaaccg tcgcctttaa tgccgttata acaggtcagc gtttcgttac ggactaaatc
    13141 cagcttgccg taataatctg ccagcgccca ggtttccgct ttatcaatcc acatcagcgg
    13201 cgtttcaaaa cgaatatctt tcgccatgcc caaactgacg gcatggttta gtgctttcac
    13261 aaactcatcg cgacaatccg ggtagccgga gaaatccgtt tcgcagacgc cggtaattac
    13321 ggcttctgct tttacctgat acgcatatat tgccgccagc gtcaggaaca aaatattacg
    13381 ccctgggaca aacgtattcg ggataccatc ggcttcaggt tcataatcag gcaccggaat
    13441 gctgtcacgc gtcaggctgc tgaccgccag ctcgttgagc agcgttacat caagaacctt
    13501 atgcgcgcgt gcccccagtt tcagcgccag ttcgcgtgcc acgtcgattt ctgcgcgatg
    13561 gcgttgaccg taatcgaacg tcacgcaatg gacttcatca tattgttgta atgcctgcac
    13621 cagacaggtg gtggaatcct ggcctccact gaacacaacg acagcacgtt tcatagataa
    13681 tcctgcttga acaataaaag cgttatggta acgcctgcga ttaacccgga ccagctattc
    13741 attgcgacgg cgcgggaagc caggcttcgg taaattcaaa ccagccgcgc ggtgtcagtc
    13801 gcacaccgtt cacgccggga ggggcgctaa tgcgatagtg atagttgaac agcggcgtca
    13861 gcgtcgcatc tgccattaac tggctaaaaa ccgctttcag ggcattaaat cggttttcct
    13921 catcaggcat tacttgcact gcgtccagtg tcgattgcaa atgtgcatat gctggagcgt
    13981 cgaaaacatg tggccacagt ggatcgcaac gcagccattg ctccagggta tattccggtg
    14041 cttcgccaat taatctgtcg cccatcatga ggtctgcgtg tgccagtagg gtcgtgtcgt
    14101 cccagttttt tgcgttatga aaaataattg tgagttcaca gccttccgct gccagtgtcg
    14161 cctgtaggcg ttctgccatg gtatgaagtt ctatcggtag gtgataaacc agcgtcaagg
    14221 ttttcggtag tttaacttca tccggtacct gccaatgcgg aatagtccag ccaggcagta
    14281 atgcatgact ggcggtgatc aggttttctc cgacttctaa cgtttgtaat aaaccggatt
    14341 gatgaataat ggagattact tttcgcgcct gccagaggga cagtcggaga cttttgcgca
    14401 atgttaaata gcaaaaacct aaactgatgc cgctactgac ctggctgacc cgttgcagct
    14461 cctccggttt gccaatggtg atttgcacgg gatgccgaca actggttccc aaatcttttt
    14521 cgaaaagcgg cggagttatc cagtactcaa ccgctttaag cagcggatga cgtaaatggt
    14581 aataatcatg gctctccagg cgcaccagtt ccgctgtgaa ttgtgttaag cgaaaaggac
    14641 ctgtgccgat cagtgggaat tgcggatgcg ccagatggct gcaatagctc gccagtcggt
    14701 gcgcaagcca gtaatcgggg cgatgtaaaa agaaggtcag acactgcgga tgggtgactt
    14761 caatacgctt cacgctaata aataattgat ccagtgctgg cagttgtaac agcatcaata
    14821 atcgctggtg taagtgtgag gcttttactg catcgccgtt atgccagtgt agcgttgaac
    14881 gaagataaaa gtcccagcgt aacccgtcag tagaggtttc ccagtgatgt gctaaatcgc
    14941 caatcgggcg ctgagtatta ttatcgaagc gggtcaggcc ggaaaatatc tgcccggcca
    15001 gatgctgctc ggcacggccg ggcaaaaagc ccggttgtag cggttcgagc gggcgatagt
    15061 agggaatacg caacgtgggc gtatcgtttt gccattgtcc gcccataaac ggctgtaaca
    15121 gagtgcgcag ctcacctggg gccagttgcg ccagctccag cacatcttgc tgctttccgg
    15181 tttccagcgc ctgttccatc atcgcattgc gtagcgattc tggcgtgacc agaaagcgta
    15241 gttgaccgcg ctttccgcgt cctgactgcg cctgccactc cagccatccc gcctcctgtg
    15301 cctgacgcaa caccgtacga acatggcgtt cgctgcaaaa acagcgttcg gccagttcgc
    15361 tgacggtgac ggtttgcggc tttccggcgg aaggttgcca cagacgttga tactggttaa
    15421 gacggttgag cagtcgcata taaacccgga acaatattat ttaactattc actattactt
    15481 ccgtatatat caggtgatac tcaatcacca ttaactgtgt cacagagtgg agaaagaaat
    15541 ggctcgtctg gcagcatttg atatggatgg cactttattg atgcctgacc atcatttagg
    15601 tgagaaaacc ctctctactt tggcgcggct gcgtgaacgc gacattaccc tcacttttgc
    15661 cacggggcgt catgcgctgg agatgcagca tattctcggg gcgctatcgc tggatgcata
    15721 tttgattact ggcaacggaa cgcgcgtgca ttctctggaa ggtgaacttt tacatcgtga
    15781 tgatttacct gcggatgtcg cggagctggt gctgtatcag caatgggata cccgagcaag
    15841 catgcatatc ttcaatgacg atggttggtt taccgggaaa gagatccctg cgttgttgca
    15901 ggcatttgtc tatagcggtt ttcgttatca gataatcgat gtcaaaaaaa tgccactcgg
    15961 cagcgtcacc aagatctgct tctgtggcga tcacgacgat cttacacgct tgcagatcca
    16021 gctatacgaa gcattaggcg agcgtgcaca tttatgtttt tccgccacgg attgcctcga
    16081 agtgctgccg gtgggctgca ataaaggcgc tgcattgacg gtgctgaccc aacatttagg
    16141 tttatcgtta cgcgattgta tggcctttgg tgatgcgatg aacgatcgcg aaatgttagg
    16201 cagcgtcggt agcggattta ttatgggcaa tgcgatgccg caactgcgcg cggagctccc
    16261 gcatttaccg gtgattggac attgccgaaa tcaggctgtc tctcactatt tgacgcactg
    16321 gctggactat ccacatctac cttattcccc cgaataacga gatcccttcc agcaccgggc
    16381 aattgcccgg ttttttttgc gttgaatttg tcattttgtg ccgtggtgct taaaccgcac
    16441 agaataaatt gtcgcgattt cacctttaaa atagaattaa aagagaaaaa aattctctgt
    16501 ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttgctaca gcaggattgc
    16561 accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc ctgctggaag
    16621 cgtctgaaac ggctggagga cgacggtatt cttatcggca aagtcgccct gctggatccg
    16681 gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca tcacagcagc
    16741 gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct ggggttctgg
    16801 cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat gaaacgctac
    16861 gacgagtttt ataagcgtct ggtaaacagt gtaccggggc tgtcggacgt cacttccagc
    16921 ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat atccagaatc
    16981 aagtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc cgtcgggaat
    17041 ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg caactggttc
    17101 cgccaaaagt ggttggtatt gttgttgatg gcgtgacaga acaacacttt actaccgggc
    17161 agatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat ctcctgcgtt
    17221 acgtctggcg cgtattgctg tttggtgcgt cttatcaact ggctgttgaa ctgcgtgaag
    17281 attattaccg ccagctaagc cggcagcatc ctgagtttta cctgcgtcat cgcacgggcg
    17341 accttatggc ccgtgcgaca aatgacgtcg atcgcgtcgt gtttgccgcc ggagaagggg
    17401 tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg atgtctacgc
    17461 aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg gcgatcatga
    17521 tcaagcgcaa cggcgatgcc ttgcatgaac gctttaagct ggcacaggcg gcgttttcca
    17581 gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa gcctttggtc
    17641 tggaagatcg tcagtcggcg ttatttgccg cggatgccga agataccggc aaaaaaaaca
    17701 tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg attggtatgg
    17761 cgaacttgct ggcgatcggc ggcggtagtt ggatggtggt gcagggcagt ttaacgctgg
    17821 gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg ctggcgctgg
    17881 catggatgtt taacattgtg gaacgtggca gcgcggcgta tagccgaatt cgtaccatgc
    17941 tggccgaagc gccagtcgtt attgatggta gtgataaggt gccagaaggt cgtggtgaac
    18001 tggatgtgaa tattcgccag tttacgtatc cgcagactga ccatcctgcg ctggaaaacg
    18061 tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact ggttccggca
    18121 aaagtaccct gttgtcgctc attcagcgtc atttcgacgt cagcgagggt gatatccgtt
    18181 ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgt ctggcggtgg
    18241 tcagccagac accattcctt ttttccgaca ccgtggcgaa caacatcgcg ttgggttgcc
    18301 cgaatgccac ccagcaagag attgaacacg tagcgcggtt agccagcgta catgacgata
    18361 ttttgcgtct gccgcaaggt tacgacacag aagtgggcga gcgcggtgtg atgctttctg
    18421 gcggacaaaa acagcgtatc tccattgctc gagcgttatt agtcaacgcg gaaatcctca
    18481 tccttgatga tgcgctttca gcggtggacg gacgcacaga gcaccagatc ctgcataacc
    18541 tgcgtcagtg ggggcaggga agaacggtaa tcatcagtgc ccatcgcctt tctgcactga
    18601 cggaagccag tgaaattatt gtgatgcagc acgggcatat cgcccagcgt ggcaatcatg
    18661 atgtgctggt acaacaaagc ggctggtatc gcgatatgta tcgctatcaa caactggagg
    18721 cggcgctcga cgacgccccg gaaattcgcg aggaggccgt cgatgcgtag ttttagccaa
    18781 ctgtggccga ctctcaagcg tctgttagcg tacggttcac cgtggcgtaa accgttgggg
    18841 attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc gctgcttatc
    18901 agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt ggttgcaggg
    18961 ctggcggcgg cgtatgttgg gctgcaactg tttgcagccg ggctacatta cgcgcagtcg
    19021 ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat
    19081 gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca ggtgatttcc
    19141 cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt ggcaactgtc
    19201 ctgcgtagcg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct cgactggcga
    19261 atggcgctgg tggcgataat gattttcccg gtggtgctgg tggtaatggt gatatatcag
    19321 cgttacagca cgccgattgt ccgtcgtgtg cgcgcttatc tggcggatat caacgacggc
    19381 tttaacgaaa tcatcaatgg catgagcgtt atccagcagt tccgtcagca ggcgcgattt
    19441 ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca aaccctgcgc
    19501 ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat tctttgtggc
    19561 ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt gttgtatgcg
    19621 ttcatcagtt atttggggcg acttaacgaa cccttaatcg aactgaccac gcagcaggcg
    19681 atgctgcaac aggctgttgt tgctggtgag cgcgtttttg agctgatgga cggaccgcgc
    19741 cagcaatatg gcaatgatga tcgctcgttg cagagcggca ccatcgacgt cgataacgtg
    19801 tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc tgtgccttcg
    19861 cgcaactttg tggcgctggt cgggcatacc ggcagtggca aaagcaccct cgccagttta
    19921 ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg tccattaagt
    19981 acgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg
    20041 ctggcggata ccttcctcgc caacgtgacg ctgggacgtg atatctctga agaacgcgtc
    20101 tggcaggcgc tggaaaccgt gcagctggcg gagctggcgc gtagcatgag tgatggtatt
    20161 tacacgctgc ttggcgagca ggggaataat ctctcagtcg ggcaaaagca actgctggca
    20221 ctggcgcgcg tgctggtgga gacgccgcaa atcctgatcc ttgatgaggc aaccgccagc
    20281 attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg tgaacatact
    20341 acgcttgtgg tgattgctca ccgcttatcg actattgttg atgccgacac cattctggtg
    20401 cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc ggcccaggga
    20461 cgatactggc agatgtatca actgcaactt gcgggcgaag agctggcagc cagcgtgcgt
    20521 gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa
    20581 cgcactgtgc actgtcatag tgcgttttca ttttcaatct tcttaacttc ctgctctctt
    20641 tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc agaaccatta
    20701 ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa accattcaag
    20761 ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac cgtcaccgaa
    20821 gtgaaaggtt ttgggcgtca gaaagggcat gccgagctgt accggggggc ggaatacagc
    20881 gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca actcgatgaa
    20941 gtgatcgata tcgtcagcaa agcggcttac accggaaaaa ttggcgacgg caaaatcttc
    21001 gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcgctg
    21061 taatctctgg cacacagcaa caggaacgaa aaatgaagat agcaacgata aaaactgggc
    21121 ttacttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg gccgataaag
    21181 ccgacaatgc atttatgatg atttgtactg cgctggtgct gtttatgact attccgggga
    21241 ttgccctgtt ttatggaggg ctgattcgtg gcaaaaacgt gctgtcgatg ctgacgcagg
    21301 tgaccgtgac gtttgcactg gtctgtattc tctgggtggt ttacggctac tcgctggcgt
    21361 ttggcgaagg caacaacttc ttcggcaaca ttaacgggtt gatgctgaaa aacattgaac
    21421 tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag ggatcgtttg
    21481 cctgcattac cgtaggcttg atagttgggg cgctggcgga acgaatccgc ttctcagctg
    21541 tgttgatttt tgtggtggta tggctgacgc tctcttacat tccgattgcg catatggtct
    21601 ggggcggcgg tttgctggct tctcacggtg cgctggattt cgcgggtggc acggtagtgc
    21661 acattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct
    21721 tcggcaaaga ggcgtttaaa ccgcacaacc tgccgatggt cttcaccggg actgccattc
    21781 tctatatcgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaaa gaaatcgcgg
    21841 cactggcatt tgtgaatact gtggtcgcaa ccgcggcggc aattcttggc tggatcttcg
    21901 gtgaatgggc gctgcgtggt aagccttcac tgctgggagc gtgttctggc gcgattgccg
    21961 gtctggtcgg cgtgacgcca gcctgtggtt acattggggt tggcggcgcg ttgattatcg
    22021 gcgtggtagc tggtctggcg ggcttgtggg gcgttaccat gctcaaacgc ttgctgcggg
    22081 tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggtattgtc ggctgtatca
    22141 tgaccgggat ttttgccgcc agctcgctgg gcggcgtggg cttcgctgaa ggtgtgacga
    22201 tgggccatca gttgctggta cagctggaaa gcatcgccat tacgattgtc tggtctggtg
    22261 tggtggcatt tatcggctac aaactggcgg atctgacggt tggtctgcgt gtaccggaag
    22321 agcaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat aacgcgtaac
    22381 aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta attgtgatta
    22441 cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgggt ataaaactca
    22501 ccgcgcacaa agccgcgtgc gctggacgcc gaggtgcttt ccacgctata cagcagccat
    22561 tcattcaaat taaacgggcg atggaaccac atggaatggt caatggtggc aatctgaatc
    22621 cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa attaagatca
    22681 gaagcataac cgagcagata ctgatgaacg cgcagatcat ccggcacgct accatttgcg
    22741 cggatccaca cctgacgatg cggttctgcg acgtgacctt tcagtgggtt atgaaactcc
    22801 accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac tggcggcagc
    22861 aggtgcgcca gcgattgggc gatttgcgtt tctgaaggca ggccatcagg cgctggtgcg
    22921 gacggcattg ttttttgatg ttcgaaaccc gcttctggtg cctggaaaga ggcagtcata
    22981 taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa gctgttaccg
    23041 tcacgcagtg tttcgacatc ataaataatc ggcttcttac tatcgccagg gcgaagaaag
    23101 tagctgtgaa aagaatgtac cagccgctcg tcagggacgg tctcttttgc agcatacaag
    23161 gcctgaccca cgacctggcc gccaaatacc tggcgtaaac ctaaatcttc actctggccg
    23221 cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt ttttagcgcc
    23281 tgactcatat aactctccag taacaaagct gccgcagcaa gccaaagtga gttgagtata
    23341 acgcaaattt gccactggtc cgatgggtgc aatggtctga attacgggct aattacaggc
    23401 agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc actcttccgg
    23461 cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa actcgtgcac
    23521 atggccagtg gtttagcggt tgcgattgcg ttggcggctt gcgcagataa aagcgcggat
    23581 attcagacgc cagcaccggc tgcaaatacg tctatttcag caacacaaca acctgctatc
    23641 cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact gccgcctgat
    23701 gccgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc gaaagtgctg
    23761 gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt tctgccattt
    23821 aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat taccgtgaat
    23881 gacaaactgg tatttattac cgataccgtt cagccggtga tcaaccaggg cggaactaaa
    23941 gccgacctga cattggtgcc ggtgcagcaa accgccgtgc cggttcaggc tagcggtggc
    24001 gcaacgacta ccgtaccttc aacttcacca acccaggtga atccgtcttc ggcagttcca
    24061 gctccaacgc aatattaagc gaagttaacc ctctccgccc ggagagggtt agtagttcca
    24121 gcgataaagc tgcaagtcga tttgcccgct tcccgacacc atcacacctt ctgccagtaa
    24181 tgcctgtcgc tgacgctgta aatccggtcc ggtcagtgaa atggtgccgt ggcgattaac
    24241 cacccggtgc cagggcaagg tgctgccttc ggggagacgc tttaacacac cgccaacctg
    24301 gcgcgcggca cggggcgagc ccgccagttt cgccacatca ccgtaagtgg tgacatagcc
    24361 ttcgggaata gcggctacga tttgccagac gcgttgggga aatgaatctt ctttttccat
    24421 cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt gaagccgcat
    24481 tgcgcaagaa accagcatct ggcacgcaat gggttgcaat tagccggggc agcagtgata
    24541 atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt ctcccgcaac
    24601 gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc
    24661 cgggatgtag ctggcagggc ccccacccat ttctgcctcc taccatttcg tcaaaaaatc
    24721 ccaacatggc taaactttaa ccacaactga cgtcgcaaga attgtctggc tgcgcagtac
    24781 gcttcggagg tatgtctgat gaagtatgtt gatggttttg ttgttgccgt tcctgccgat
    24841 aaaaaggatg cctatcggga aatggccgct aaggccgcgc cattatttaa agagttcggg
    24901 gcgcttcgta ttgtcgaatg ctgggccagc gatataccgg atggtaaagt gaccgatttt
    24961 cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat tgaataccct
    25021 tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg gatgaaagag
    25081 ttcggcgagt ccatgccgtt tgatggtaag cgaatgatct atggcggatt cgagtcaatc
    25141 atcgacgagt aggtagcgtg acgggctgtg cggcgtgccg tcagccctat aattcacaaa
    25201 tgttgctcgg cccagcgtaa gaaatcctct ttcggtaatg ccttgctgta gagccagccc
    25261 tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt ttcgattccc
    25321 tccgcaacca ttttcagttt cagtgttttt gccatttcga tgatatgtgg cgtgacattc
    25381 ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac atccagatcc
    25441 tgtaaatagc ttaaacttga ataccctgta ccaaaatcat cgagataaat ttcatggccc
    25501 gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa ctcgcgttca
    25561 gtgagttcaa gcgcgatctg tctgggatta acctgatagt gattgatcat ttcacgcagc
    25621 aattgcggga ttttttccga ggtgagcacg gtggattcaa gattgatcga aatatgctgc
    25681 tgaggatgct gacgcagcca gtcgcccata tcttcaaaga cgcttcttat aatcagtagc
    25741 gtcaatggct cagaaaggcc cgtttgctgt gccagcggaa taaaactatc tggtgacaac
    25801 caactaccgt ctgtctgcgg ccagcgcgcc agtgcctcag caccgacaat tttgccattg
    25861 gctaaggaga caatcggctg atagtgcacg caaatatcac gattttcgat agcatcctgc
    25921 agccgatgat gcggtgactg gatacggcgc aaaatacgca gcacaaacat cgctgccagc
    25981 aggccaatca ccaacccgag cggtaaccaa ataaagactt gccgatacca gcctttctgc
    26041 aacattttcg ttgaagccca ggtgatgatc gaaatattca tctccggtaa gggcaggata
    26101 tcgtaaatga ttccattatt ttcgatatgc tcaccgggtg ttttttgtag cctggtaata
    26161 attccctgag caagttcatc gctgctggtt atgacaacgt tatgggcatt gccaataatg
    26221 gcggcatcaa tttgccatga gctataggga atgacatcaa taaaggaagc ggggtcgatc
    26281 atgacgacat aatgtgccgt tcccatggcg accatgtaac ggataatgcc taaatcgtta
    26341 tgcgatgtta accagacacg ataaccatct ttcgaaattt tacctggctc ggggaaggta
    26401 tcgggcgggc tttcatgctc cagagacgaa cattggggaa cgttgttatc gatataaacc
    26461 acttcctgaa tatagcgata actgtaagat acccgacgca tttccatgag atgggcttcg
    26521 ctacaggcag cgccttgcca tctttccagc tcttgcagcg catctttccc ttgtgtcgcc
    26581 accttattgg cccgaatagc gacgcgggag gaatacgtat tcagctcttc aataaatgac
    26641 gtttctacct gctgatgggc cagccagatg cttaagccga caggcagcaa tactgaaaga
    26701 atcagtactc ccgaaataag gccgaccaga tgtcgtgttc tcaccagcat atccttatta
    26761 acacagtgta gggaaagtat atctgattgt ggaaaaaggc tggcaggaga taaggaggta
    26821 gagctggtat ttcatgaagt gatgggtgga agcaatcgtt gtcatttgac caggcgtaga
    26881 gctttacgcc tggtatgtac ccctaactta agatgttgat tcggctcgag cgagggtaaa
    26941 aatatcgtaa aaagatagtt cacctttacg gacgatcatt ttttgcagtt gttctttatg
    27001 aacatccgcg acacgtggag agttcaggat ggcttcaacc agcgcggcag gtacaaagcg
    27061 cgtgttgtac cattcgccgt tatagcaaac gcggaaatcc agcacgtcga tatcttcata
    27121 gcggtagcgt gggtagacat cgaaaaagaa gaaaccatta agcaggataa ataatacaac
    27181 cagtagccag acagagcctg aaagcgtttc ggattgcaac atcaccgcca gggtagcaaa
    27241 aaaagcaacg tacataccga taaacagccc cggatgttta cggataaaac tgatactaaa
    27301 gcggggtttg ttgtcgcgtt tttcgcgggt attcagagac tcaatcgttt cggtcagcag
    27361 gcgttgtatt tcggtcatcg tttgccttcg tgatatctgc aactttccag ggaacaccct
    27421 attttaacgt gggaatagta gaggggaagt aacagatttg ccacgatttt gcataacagt
    27481 tgagaaaagc tggttacaat tttttaatga ttctggctgg attaccgccc acgacaacgt
    27541 tgtccgggac atcttttgtg acaactgcac ctgaggctac cacgacgtta tcaccaatgg
    27601 tcacaccagg gttaatgact gcacgtccgc caatccagac gttattaccg atggtgacgg
    27661 gtttccccag ttcagcaccg ctattacgtg ctacagggtc gatgggatgt gttgccgtgt
    27721 agatatgaac gcctggtgcc aacatacagt tatcaccaat gcgaataggg cagacatcaa
    27781 gcatcacgca atcgaagttg gcgaaaaaat tattgccgag aaaaatgtta tagccatagt
    27841 cacagcgaaa cgttggctca atataagcct ctgtcacctg accgaataaa tcagcaagaa
    27901 tttgctggcg taatgtgtgc tcttccgcca gggaatgatt gtatcggtga ataagccgac
    27961 gagcgcgcag gcgatcgcga gataacgcct catctgccga gtgatacaac tcaccagcaa
    28021 tcatcttttc tttttctgtg ctcattactg acttccggtt gctgaaaatg cggcaacaca
    28081 gtaagacgaa aagtcagtga agagaacgtt cccattacat ctgtgtgatg aagatcacat
    28141 agggtatgcc ttcatggcga aaaaaagtat aaaattctta ataaacagcc ggttatagct
    28201 ccgaaagcga gttatttatt agcgaataaa tttccatact gaggaaggga tcttgtcgta
    28261 cagtttattc atggtcaatt cggcgaggcg gtgatctgcg gctgagtaaa ataccgccag
    28321 ttcattatct gataattcgt atttattttt ctcgataaca cgctccagcg tgtcaattgt
    28381 ctggcaacga cgtaaacgca ttaaataatc ggttttcgtt aaaggttttt cggacatact
    28441 tctacccatg gttgtaataa ttctaacaag ataaactcgc aggattctct ttcgtcgcat
    28501 tgacaaaaca acggaatagt cgattacctg acttccgcca tttctgaaga tcctgcatat
    28561 taataccata actactgaac aacataaagg tgtcatccag atattcgtcg atctgctcaa
    28621 tgagcttatt gtcttcatta tacttaattt tgtaattaag tgcgaaggtc gcaatatgct
    28681 caatcagttc attcaactgg aggttgatcg ccgaggttgg gtcgttaacc cagccatgat
    28741 tgctttcttc aaggtttgca aggcagtcat gatacagggt ttcacagaga aacttaagct
    28801 gtgcgatatc atgtcttttg ggtgagtatt catccataac gcgtcccctt cttagcggtt
    28861 gaactaacgg acacctttcg ggatggaaaa aacttactga cctggacttg ccctctcgtt
    28921 tgttagctta actataagcc actctttgca ggttttcatc gcattattac gaaaaattac
    28981 aattaggaag gaagcgggac aggactcact ttttcatact agcaccaacc cgcagcaggt
    29041 aaaagcagtt tgctcgaact gttcataaca tttcagaatt atcgccagaa gaaattggtg
    29101 acactaatac caggattgct ctgaatatga cgtaataacc gaggaatgaa taaagaatta
    29161 ccgcaaataa ttaagaatag cctctaaatg gttatggatt attaaatcta ttagcgctac
    29221 ttaa
//
LOCUS       DACADA010000047        29151 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.048, whole genome shotgun sequence.
ACCESSION   DACADA010000047 DACADA010000000
VERSION     DACADA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.048"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(4..1863)
                     /gene="gss"
                     /locus_tag="ISB17_002030"
     CDS             complement(4..1863)
                     /gene="gss"
                     /locus_tag="ISB17_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAP1979963.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2068..2934
                     /gene="yghU"
                     /locus_tag="ISB17_002031"
     CDS             2068..2934
                     /gene="yghU"
                     /locus_tag="ISB17_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAP1979964.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3004..3252)
                     /gene="hybG"
                     /locus_tag="ISB17_002032"
     CDS             complement(3004..3252)
                     /gene="hybG"
                     /locus_tag="ISB17_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAP1979965.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3265..3606)
                     /gene="hypA"
                     /locus_tag="ISB17_002033"
     CDS             complement(3265..3606)
                     /gene="hypA"
                     /locus_tag="ISB17_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP1979966.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3599..4087)
                     /gene="hybE"
                     /locus_tag="ISB17_002034"
     CDS             complement(3599..4087)
                     /gene="hybE"
                     /locus_tag="ISB17_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAP1979967.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4080..4574)
                     /locus_tag="ISB17_002035"
     CDS             complement(4080..4574)
                     /locus_tag="ISB17_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAP1979968.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4574..6277)
                     /gene="hybC"
                     /locus_tag="ISB17_002036"
     CDS             complement(4574..6277)
                     /gene="hybC"
                     /locus_tag="ISB17_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAP1979969.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6274..7452)
                     /gene="hybB"
                     /locus_tag="ISB17_002037"
     CDS             complement(6274..7452)
                     /gene="hybB"
                     /locus_tag="ISB17_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAP1979970.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7442..8428)
                     /gene="hybA"
                     /locus_tag="ISB17_002038"
     CDS             complement(7442..8428)
                     /gene="hybA"
                     /locus_tag="ISB17_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAP1979971.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8431..9549)
                     /gene="hybO"
                     /locus_tag="ISB17_002039"
     CDS             complement(8431..9549)
                     /gene="hybO"
                     /locus_tag="ISB17_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAP1979972.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9738..10025)
                     /locus_tag="ISB17_002040"
     CDS             complement(9738..10025)
                     /locus_tag="ISB17_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAP1979973.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10144..11031)
                     /locus_tag="ISB17_002041"
     CDS             complement(10144..11031)
                     /locus_tag="ISB17_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP1979974.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKSNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11188..12228
                     /gene="gpr"
                     /locus_tag="ISB17_002042"
     CDS             11188..12228
                     /gene="gpr"
                     /locus_tag="ISB17_002042"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP1979975.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12268..12762)
                     /locus_tag="ISB17_002043"
     CDS             complement(12268..12762)
                     /locus_tag="ISB17_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAP1979976.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            12953..13837
                     /gene="yghA"
                     /locus_tag="ISB17_002044"
     CDS             12953..13837
                     /gene="yghA"
                     /locus_tag="ISB17_002044"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP1979977.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13876..14301)
                     /gene="exbD"
                     /locus_tag="ISB17_002045"
     CDS             complement(13876..14301)
                     /gene="exbD"
                     /locus_tag="ISB17_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAP1979978.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14308..15042)
                     /locus_tag="ISB17_002046"
     CDS             complement(14308..15042)
                     /locus_tag="ISB17_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAP1979979.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15294..16481
                     /gene="metC"
                     /locus_tag="ISB17_002047"
     CDS             15294..16481
                     /gene="metC"
                     /locus_tag="ISB17_002047"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAP1979980.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16621..17280
                     /locus_tag="ISB17_002048"
     CDS             16621..17280
                     /locus_tag="ISB17_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1979981.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17320..18219)
                     /locus_tag="ISB17_002049"
     CDS             complement(17320..18219)
                     /locus_tag="ISB17_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1979982.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18413..19576
                     /gene="yqhD"
                     /locus_tag="ISB17_002050"
     CDS             18413..19576
                     /gene="yqhD"
                     /locus_tag="ISB17_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP1979983.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19681..20508
                     /gene="dkgA"
                     /locus_tag="ISB17_002051"
     CDS             19681..20508
                     /gene="dkgA"
                     /locus_tag="ISB17_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAP1979984.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20708..21634
                     /locus_tag="ISB17_002052"
     CDS             20708..21634
                     /locus_tag="ISB17_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAP1979985.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALILNDNLTLPGNIGWLDYDPVCDCQDFGNLVLESVAITQTD
                     ADHADAAVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     TIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21685..21942
                     /gene="yqhH"
                     /locus_tag="ISB17_002053"
     CDS             21685..21942
                     /gene="yqhH"
                     /locus_tag="ISB17_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAP1979986.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(21984..24203)
                     /locus_tag="ISB17_002054"
     CDS             complement(21984..24203)
                     /locus_tag="ISB17_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAP1979987.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKS
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICSHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24455..25204
                     /locus_tag="ISB17_002055"
     CDS             24455..25204
                     /locus_tag="ISB17_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1979988.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIISLQENAIAAESDRFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25527..26999
                     /locus_tag="ISB17_002056"
     CDS             25527..26999
                     /locus_tag="ISB17_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1979989.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDVVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            26996..28012
                     /locus_tag="ISB17_002057"
     CDS             26996..28012
                     /locus_tag="ISB17_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1979990.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREKKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28023..29033
                     /locus_tag="ISB17_002058"
     CDS             28023..29033
                     /locus_tag="ISB17_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HAP1979991.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDADTAQQVTDVLIHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHTIK
                     LALEEGLGFVSVKNTSHCGALSYFAEMVTNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKDGYGTTDPHKIE
                     NLLPFGQHKGSGIALAIDALTGMLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAQMVTELHAVKPAPGIEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
BASE COUNT         7352 a         7592 c         7251 g         6956 t
ORIGIN      
        1 gtattacttt ttcaccacaa ttaacggttc aatatcactc tcttttttga tgaccagtga
       61 ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca acggtgaagg tacatacctg
      121 aatgtattta ccgtccactt tcggcaaaca ccacagttgc tggtaaatat ttttctgttc
      181 ggcaaattta ccactggtct gatcaagcac ctcttcatga tggctgacca ggtcaatgtt
      241 gctaccacag cgcccggcaa tcggtttaac ggcataaccg gttttcacca gctcatcatt
      301 cacggtgaag tcagtatcca acagataacg atggtgcggg aacagagacc agaggatcgg
      361 cagaatcgct ttgttaccgg ggatcaccgt ccatagcggc tcaaaaacca acacttccgg
      421 gcgcagcaat acgtcgataa gacgcacttc attttgtgga tgcccggtac ggatcggcac
      481 tgcggcaaat tcacggtcgc tcacttcgcg gatttgatca aatgccgttt cccacgccca
      541 ggttttccat acgcagttaa ccagtcgccc ttccccatca atcagttgcc cggcagcatc
      601 ccagcccagt tcatccaacc cacgcaagat acgcgtttca aagcccgcct ggtgcagcgc
      661 ctgctccata aactgcgcgt gatagttttc ctcgatatct ttgtcctgca tgatatggac
      721 aaacggacgt gcacgactgt gtttccacgc gccagccagt tcgttaatca gtccttccgc
      781 aggattgaag ccgttgcctt tatagccctg ctccgcccaa cgttcgagga tcaagcccgc
      841 ttcggtatga caggaggcag aatcggcgtt gtattcgtag actttcaggc cacgttcatc
      901 catgcagaaa tccatacgac cagtgatcat atgatgacgg cgacgctgcc aggagagacg
      961 caaacgtggc cagaggattt tcggaatgtc aaacagcgcc agcaggttgt catctttcag
     1021 caccttgtcg gtggcgtgaa gatacatcag gtgcagctcg ttggtggctt taatcagctc
     1081 ctgctcggca ctctcggtaa tggtgtagta atgatacgga tcctgattga tcacctggcc
     1141 gttggcctgt acataggcgt tttgcagcgg atctttttca tccagccatt taccgtcaaa
     1201 ctggcctttg ttttccagac gcgccccgct gattttcagc agttcgcctg caatttccgg
     1261 ctgtggcaag ctgtattcgg tatcttccgt ctggatcatc cagcccagaa tagtggtgtc
     1321 atcgaaggtg tctttcaggg tatagccgcc gttttcgacc accatctcca gctcgcgtgt
     1381 ccactgttgc ccttgcggca acggggtatg aatcacgttc tgttccgcaa tacggacttt
     1441 gttgccatgc aattgggtaa tgatggcgac atggccagtg tctttaaatt caccgccttt
     1501 atcccagata agaagcgccc ccgctaccgg tgcacgcggc gaaccgttag gaaatgcctg
     1561 caatggcagg atgttgtcat tgaccacttc acgcaggaag cgcagtgaga aaatctccca
     1621 tgccataccc acgtcagtga agactacacc gtagttcaga aagagaaaac ggcgagcaaa
     1681 ttcaacgcat tgccatttgt ggcccatata ttcatcgtcg atatagctac ggaatacggc
     1741 gtcatcttcg tattcctgcg gatcgagaga actgtaatct gaagagtaga ttgctacccc
     1801 acctggggcg tagcccaata atgtcccgaa cggggcatcc tggctggtcg ttcctttgct
     1861 catcacttta cctttaacaa tacaacctaa gcagttggcg taattgttgt gctctgatta
     1921 cctgctcagc gacattaacc ggacagaggt cagactaatc atacacctca tcgcagcggc
     1981 tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag tgacctgcta cactgcttca
     2041 acactaccac tcagaaggca actcactatg acagacaata cttatcagcc cgcgaaagtc
     2101 tggacgtggg ataaatccgc tggcggcgcg ttcgccagta tcaatcgccc ggtttctggt
     2161 ccgacgcatg aaaaaacgct gccggtgggc aaacatccat tgcaacttta ttcgctggga
     2221 acgccgaacg gtcagaaagt aacgattatg cttgaggagc tgctggcact gggcgttact
     2281 ggtgcagagt acgacgcctg gctgattcgt attggcgatg gcgatcaatt ctccagcggc
     2341 tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc gcgaccatac ccataatcca
     2401 ccgatccgcg tgtttgagtc tggatcgatt ctgctttatc tggcggagaa atttggctac
     2461 ttcctgccgc aggatttggc aaagcgtact gaaacgatga actggctgtt ctggttacag
     2521 ggcgcggcac cgttcctcgg cggtggtttt ggtcactttt accattacgc gccggtgaaa
     2581 attgagtacg ccatcaaccg ctttaccatg gaagccaagc gcctgctcga cgtgctggat
     2641 aagcaactgg cgcagcacaa gtttgttgcg ggcgatgagt acaccattgc ggatatggcg
     2701 atctggccgt ggtttggcaa cgtggtgtta ggcggcgtgt atgatgccgc agagtttctg
     2761 gatgcgggca gttataagca cgtgcaacgc tgggcgaaag aagtgggcga acgtccggcg
     2821 gtgaagcgtg ggcgtattgt taaccgcacc aacgggccgc tgaacgagca gttgcatgag
     2881 cgccatgacg ccagtgattt cgagacgaat acggaagata agcgtcaggg gtaagtcgca
     2941 acattggcag gcttatcgct atgtagccgg ataaagcatt tacgccgcat ccggctttaa
     3001 tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc
     3061 ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag
     3121 cagatcggca gggttacctt cacaaatcag ggcgatattc acgtcgcgct tgataccaca
     3181 tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac
     3241 gccaatacac attgttaact ccagttattc aacttcaata cttttgacga tcagcgaatc
     3301 gccggtatcg acacgcaacc gctcgccatg acagagcgga cactgcgcat cgtgctgatg
     3361 aatctccacc acctggctgc aatcccagca ccaggcctgg gcgggtttat agacgatatg
     3421 taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt tcaaaactaa aacggacggc
     3481 gctctcctca acgcaggaga gcgcgccaat ttccagccac acggcggtga cgcgcttaac
     3541 atcgtgctgc tccgcctgcc gttggataat ttcaacggcg ctctggcaaa gcgacaactc
     3601 atgcattttc gccactcctg cgaccaaaca gcagggcgcg acgccctgcg tgtggtacat
     3661 ccggattagc gaccggtagc gaaaggatca tccgcgcgca gtcatccgtc aggcgttggc
     3721 cctcttcaat cgacatgcta tgcgaaagcg gtgacatcag cgaacaggag agatattgag
     3781 aaacactgtc cagttcgcca acggtaaagg tcatagtgcc atacggcagt tgcagaccaa
     3841 ttttttcact cactttgcgc agcggccaga gttgatccgg gccggggaag ataactgcac
     3901 tcagcatcca cggggtgatc acacaccccg tccactgacc ttcgaacagc gtaaagtcag
     3961 aaacatacac cggcattgag ggatgcagaa aagagagatc gtgcatcgaa cgccgggcaa
     4021 tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt ctggaaacct gctatctctt
     4081 cagtcatgag tcgcctcccg tgggatagct tccacgcctg attcgcgcag cgcagccaga
     4141 acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg gcgttaagcc gatgtgcggc
     4201 tccagcgatt ccgggatcac accgaccagc gtcagctttt tcggaaactc gccggtgaag
     4261 cgcagggccg acaatacgtc ggccaggcca agctgatgcg gagagatttt gttggtaaac
     4321 aacgccggaa cttcttcatc ccgcaggatc atcatcgttc ccggcgcgtt ctttttcgac
     4381 acaatggcat ccgcaataat cagatgatcg cgatttgcca tgtcgccaag cagctccatt
     4441 cccgccgtgc cgccatcgag gatctcaaca taatccggca gaatgtatcg ttgctctaac
     4501 gcttcaacaa tccgcacacc gatggcttca tcggtcagca aaatattgcc gacccctaag
     4561 actaaaatac gcattacaga accttcactg aaaccacttc gttgccgtcg gcatccacta
     4621 cgtgtaccgc acaggccatg cacgggtcga aggagtgaat ggtacgcacc acttccagcg
     4681 gtttattcgg atcggcaacc ggtgtaccca ccagcgactg ctcgtaagga ccgacgtcat
     4741 cattgaagtt acgcggacca gagttccagg ttgatggaac aaccgcctgg tagttgctga
     4801 tgataccgtc tttaataacc atccagtgag agagcatacc gcgcggcgct tcgaggaagc
     4861 caacaccttt gaactcaccc gttgccggaa tgttcggctt cacaaaggtg gtgtgatcgc
     4921 ctttgccgat attggtgatc agtgcactgt attggttttg caggatatcc tgcaattcac
     4981 agcagtgaac ggtacgacca ataatacggc ccagcgtgga gtgcagctgt gccacttcca
     5041 gcgtgttgcc agtcagtttc tgataaatcg caacgatttc attcagtttg ttctgggtag
     5101 attcgcgacc tgccgccagt ttcaccagca tattagccag cggacccact tctaccgttt
     5161 tgccgtagaa agtcggcgat ttcacccagg aatatttacc gtcatcagac caaccgtcgt
     5221 aagccggaat ggtggtacct tcccacggtg cctgcggcgc ttcgtcttta taccaggagt
     5281 gcttcgcgct ttcctgaatg cctttgatca ggtattcatc ggaatgagaa gtgatcggac
     5341 gatacgagga cagatccgca ttctcaatgt agccgcccgg gaacaggaag ctgccgtttt
     5401 tgctgtcggt cgggaattcc ggcacgctca ggtagttcac cgcaccttta ccgcgcgtca
     5461 gccattccgg gtagaacgcg gcgataactg cggtatcaac cttataaacc tgttcaacaa
     5521 agtcgctcag cttgtcgatg aaagacttga tgtacatcag gcgctcaagg ttcagcacgc
     5581 ccaaaccgtc gaggttgatt gggttagcga ccccgcctac cgccaggttc tgaatgtgcg
     5641 gcgttttacc gcccagcagc gccacgacgc ggttagcgtc acgctggcac tccagcgcct
     5701 gcaggtagtg cgctaccgca atcaggttca cttccggcgg cagtttcatc gccgggtgac
     5761 cccagtagcc attggcgaaa atacccaact gaccgctggc aaccagatct ttaatcttgt
     5821 tctgaacttt ggtgaactct tccggactgt tcagatgcca ggtcgaaacg cctttcagca
     5881 tttcagaggc tttggttggg tcagcttgca gtgcagaagt gatgtccacc cagtccagcg
     5941 ccgaaagctg atagaaatga acgatatggt catgcgtggt gtgcgcagcc agaatgatgt
     6001 tacggatgta ttgcgcgtta accggaacgt cgatattcag cgcactttct gccgcacgaa
     6061 cggaagacag cgcgtgagtg gtagtacata cgccacagat acgttgcaca atcatccatg
     6121 catcgcgcgg atcgcggttt ttcacgatct cttccatgcc gcgccacatg gtaccggaag
     6181 cccatgcttt cgaaacgacg ccattttcga tttcgcaatc gatgcgtaaa tgcccctcaa
     6241 tacgggttac cggatcaata gtaattctct ggctcatgct ttgctcgcct catgacgatt
     6301 atgatcgttt tgttttaaag gaggaagtat cggcagtaga cgaatgagta cgatgtaagc
     6361 gcaaatctca atagccacaa aaccaataga aatcaacagt tcttcccagg tcgggaagta
     6421 ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag gtcagacgcc aggttgcaca
     6481 acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg gaatcattac gcagcttcgc
     6541 cacacgcaga acgaccagcg ggaagagcat cagcaggact tcaatccaga acatcacgga
     6601 gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga tagataagct cgccaaagcg
     6661 cagcacgatg aaaatcgcca gcaacacact gatggtgttg gtcagcttga cgaacaggct
     6721 cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc agtgaacctt caaagatgac
     6781 aatcgagaag cccatgatga acgccgtcag cagcgagaac agcggcaaca tttcatagct
     6841 ctgccacagc ggatgcacct tgtagcccgc cgagatcatc agcgacccca ttgaagactg
     6901 gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag aacatcacct tgttcagacg
     6961 ctgtagcgac accttccagc ccagacgttc aaacagtgcc ggagcaaact ccagtgccat
     7021 cacgccgata tagatggtca tacagaccgc cgtctcgaac agtaccgagt tcacgttgaa
     7081 gtgacccgga atgtagaagt acggcaggtt ccagtagcga cccacgtcga tagtgatcga
     7141 caagccacct agtgagtaac caaacagact cgccaacagc gccggacgca ccagcggatg
     7201 gtattgccca cggttaaaga cgtataccgc ccacgccagc gcccagccgc cacaggcaaa
     7261 gccggtgccg atcaacaggt caaacgcgat ccacacgccc cacgggaagc cgccgttcag
     7321 gtcagagaca gagcccagac cgaacaccag acgcttcaca atcaggagca tacagatgac
     7381 gattaacggt ccaaaaatca tgaccggttt actgatgatt ttgccgccca gcggttgtgg
     7441 atcatgactc atgatcgtct cctccgtcgt gatggtcgtt tttggtgttg cgacgaacca
     7501 gcacggttaa gcccgccagc acagccagtg gtagcatcat gcctttatac agggtgtgtt
     7561 gaatattttc ggaacgcgca ccggtagaaa gatcgtccag tttcggcagg tcgagatttt
     7621 cataaggcac acccgtcagt accagaacct gagtaccgcc gccctctttc tcgccgtaca
     7681 gatgcggata atatttcggc accgtatgca ggtaagtgtc gccagatttc agcgtctgac
     7741 gcggatagtg gtattcgctg ccaggcttca gcgccagacg ttttttcgcc tccgccatca
     7801 gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca cacttctacg cagccaggca
     7861 gaccgccttt atcgagacgt tccacacctt tctggttgca caactcgcac ttatgcagcg
     7921 caccaaacgg gttgttgtag tcgtacttcg gcacgttgta tgggcaggcg accatgcagt
     7981 aacggcagcc ggtgcacaca tctttgtcgt aatggacaat gccggttttc ggatcttttt
     8041 tcagcgcaga gaccgggcac acagagacac agttcggatc gacgcagtgc atacactgtt
     8101 tcttaatgta cgcgtagcca ttctcctcct ggtctttgtt gacccctgtg ccgctggtcc
     8161 acacctgaat gatgttattg gtatacggcg acagtttgtc gttgttcgac caggtctgtt
     8221 ccccttgcgg gttacgttca ggggagttga tatcctgaca cttggtgacg caagcctgac
     8281 agcctacgca caaggtcgag tcgtacaaca tccccagcga tcccggaatt ggcgggcggt
     8341 tttcagcagc cgcatgactg acagacggca gcgcgcccgt cagcaatgcc ccgcaggagg
     8401 ctgctttaat aaaattacgt ctgttcacgg ttattctccc cgtgagtcag cgttatcttt
     8461 cttttgctga cgacccagtt cacgcaccgc catcacgctg acaccggcaa ccagcccaac
     8521 cactccgccg agcaaaccaa tagcgcctgc cgagacgttg ccgccttctt tagcgttaac
     8581 gtccggtttc tgtgaacgcg gagtctggtt ttcgacgttg gcaagctgat ggatgccttt
     8641 atggaagccg ataccttctt cgttacagcc atagcacggg tgaccaatcg ctaccggcca
     8701 cacgccgcca acatcgcaga attgcagcgt tgagcagttg ccgtaagttt ctggcccttt
     8761 acagccgagg tggtacaggc accagccttc gcggtggcct tcatcaccga actctttggc
     8821 aaaacgacca gcatcgaagt gcgggcgacg ttcacagtgt tcgtgaatca gacgaccata
     8881 ggcgaaagtc ggacggtttt tgtcatccag tttcggcggt ttgccgtaag tgatgatgtg
     8941 cgcaacggtc gccaggaagt tgtgcgggtt cggcgggcag ccaggaatat tgataacggt
     9001 tttgcccggt agaacttctt gcaggctgac tgcgccagtt gggttaactc cagctgcggc
     9061 aacaccgccc cacgcagagc aggaaccgat agcgataatg gctgctgcgc cttccgccgc
     9121 tttgcggatg tgatccacaa tcggctcacc ggcaaccatg caataaatac cgttatcttt
     9181 taatgggatg gaaccatcca ccactaacac atactgccct ttgtacttct cgagagcgtt
     9241 atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa agcacttcgt gatactccag
     9301 agagatagtc tccagcacga ggttttctac cgttggatgc gttgcacgaa gcagagattc
     9361 cgtacaaccg gtgcactcct gcgcgccaat ccagataact ggcggacgct gcgggttagt
     9421 aaccgattcg accatctctg cagcggcttt gctacttaac cccatggtgg cggctaatgc
     9481 tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga gaatggatga gggtgttatc
     9541 tccagtcatt tatagttatt ccgttgcgaa gacctggcat atattttgcc tcaatcgcaa
     9601 aatcaataat gcgatcgatg cgccatttac cacacattta ttatggttat cgatatcatg
     9661 atactgcggc ggggcactaa aacgaaggga agaagcatta gtgtaattaa ttaaagcaag
     9721 ataatacgta tgtttgatca attttctagg cgataaccgg ccataaaaaa gcgcaacgta
     9781 gaacaggaat tattctcacg aaagaaatcc attaccatct ctttatccag tccataacgg
     9841 cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat ctccggtctt ttcgtacatc
     9901 cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg cacagaattt tgtcacatta
     9961 accgcactgt cggcatgcgt tgcttttaat cccgccatta agcctttacc aaaatggtta
    10021 ttcatcgttc acctcattcg ctatatattg tcatatatag cgatatttta ggcaaagaac
    10081 gacatttgcg cacgctattt tttcatgctt gtttaagacg taatcctgta agagataaac
    10141 ccatcaggag agatatttat cgaaccattt cagcgtcctt tgccaggcaa gatcggcggc
    10201 agatttgtca taacggggcg tggaatcatt atggaatccg tgattaaccc ccggatatat
    10261 atacgcctca tagaccttat tattggcttt caacgccgcc tcgtaagcgg gccagccctc
    10321 gttgattcgg ctatccagtt cagcgtagtg gagcagtaaa ggcgcttcaa tcttcgccac
    10381 atcggcagtg ggtgcctgac gcccataaaa cggcaccgcg caggccagtt ccggatacgc
    10441 gactgctgcc gcgttcgata cgcccccgcc atagcaaaat ccgctaatac ccactttgcc
    10501 tgtcgcttgc ggatagcgtt gcataaactc aattgcggca aagaaatcat tcatcagttt
    10561 ggttggatca acctgctgtt gcagttcgcg gcctttatca tcgttaccag gatagccacc
    10621 tactgagctt aaaccgtctg gcgccagggc gatgtaccca gccttcgcca ctcgccgcgt
    10681 cacatcttcg atatacggat tcagccctcg attctcatgc accactacca cggctggcgt
    10741 tttgccgctc atttttgcgg gcttcaccag ataaccccgt acctcgccgt gaccatttgg
    10801 cgagggatac gtgatgtact cagcaacaat ttccgggtcg gtaaactcta cctgagtcgc
    10861 caacgcataa tttgacttga gcaaatcaaa cagcgctaat gccgtcatcc cgcccaccgc
    10921 atacttcgcc gcaaggttga ggaactcacg tttcgagatt ttcccgtgag cgtaatagtc
    10981 gtagtaatcc agcaactctt gtgggaaatc tttggcggtt aaacgcggca tcgttgcact
    11041 cctcaactgg tgttttttta agcaaagcat aagcgcgtgt ttttgcccag tttttcgtca
    11101 ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc
    11161 gaaattgaac gatggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg
    11221 cagatgcagt accgctattg cggaaaaagt ggtttacgcc tgcccgcgtt atcgctcggt
    11281 ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcgat cctgcgtaaa
    11341 gcgtttgatt taggcattac gcactttgat ttagccaaca actacgggcc gcctccagga
    11401 agcgcagaag agaactttgg tcgcctgctg cgcgaggatt ttgccgctta tcgcgatgaa
    11461 ctgattatct ctaccaaagc cggatacgat atgtggcccg gcccatacgg ctctggcggc
    11521 tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat
    11581 gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct
    11641 gcgctggctc atgctgtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg
    11701 ccagagcgga cgcaaaaaat ggtcgagttg ctgcgcgagt ggaaaattcc gctgttaatt
    11761 catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc
    11821 ctgcaaaata acggcgtggg ctgcattgcc tttactccac tggcacaggg attgctgacc
    11881 ggaaaatatc tcaacggtat tccgcaagat tcacggatgc atcgtgaagg gaataaagtt
    11941 cgtggtctga cgccgaaaat gctcaccgaa gccaacctca acagcctgcg gttattgaat
    12001 gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa
    12061 gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact tgaggagaac
    12121 gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat
    12181 atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg ataaatgacc tgttaataac
    12241 gggcgcgaga accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata
    12301 gcccatcaca aatgcagaga gcacaaacgt cagatggata atgacgtacc acatcagttt
    12361 gttatccggg acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc
    12421 cacaatcgac gctgctactt tgtttttcag cgacgttgca tccattttcc ccagccagtt
    12481 cagcttctct ttgttctcgg agatatccag ttgtgagacg aaattctcgt aaccggaaaa
    12541 catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag
    12601 gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa
    12661 gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag
    12721 ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct gtcgtgtagt
    12781 taaaacgagc aacagtatat cgcaattaag taaacagcac gcaacagcaa atcaacatat
    12841 acgttaaacc tttgtcgcat aattctgagg gttagggatc ttcctggttc ccttatcaac
    12901 gcatcgagag cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa tgatgtctca
    12961 tttaaaagac ccgaccacgc agtattacac tggtgaatat cccaaacaga aacaaccgac
    13021 gccaggcatc caggcgaaga tgacaccggt accggattgc ggcgagaaaa cctatgttgg
    13081 tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg gaatcggtcg
    13141 cgctgccgcc attgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt
    13201 ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac gcaaagccgt
    13261 tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc acgaagcaca
    13321 caaggcgtta ggcgggctgg atattatggc gctggtagcc gggaaacagg ttgccattcc
    13381 tgatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta acgttttcgc
    13441 gttgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac
    13501 cacttcctca atccaggcat accagccaag cccgcattta ctggactatg cggctacgaa
    13561 ggcggcaatt ctgaactata gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg
    13621 ggtgaatatt gtcgcgccag gcccgatctg gacagcactg caaatttctg gcggacaaac
    13681 gcaggataag atcccgcagt ttggtcagaa aacgccgatg aagcgtgcgg ggcaaccggc
    13741 ggagctggcc cctgtatatg tttacctggc aagtcaggag tcgagctacg tcaccgcaga
    13801 agtgcacggt gtctgcggcg gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc
    13861 aaccgggcat catttttatt tcgctttcgc agtttcttcg ccgaccagac ctattttcag
    13921 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    13981 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    14041 aatcattgtt tcatcggtga ccggatcgtt accaataaac atcgagttgt ctgctttcac
    14101 cgacagataa accggttttt ccgggcgcgg ctgcggcgta ctggtagaag caggcaaatt
    14161 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcaaaaccaa
    14221 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    14281 ttcgttaaga tgcatcgcca tcggacatta tcctgcgcgt aatttttgtg cgacacgaac
    14341 cggatgtgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    14401 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    14461 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    14521 aatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    14581 gttcatgatg ccccagaccg taccaaacag accaacgaac ggcgaaatcg cgccgatggt
    14641 tgccagatag ccgttaccac ggcccatttg acgacctact gcggcgaccc gacgctccag
    14701 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    14761 ctcattctgc gcttcattga gcaaatgcag gctcaagctt ttgctaccaa aatcagcggc
    14821 gatatcgttg gcctggttta aggaacgcgc ttcagccagc aattgctgct cgcgcttaag
    14881 acgacgcttc tgattgaaga actccacgct cttactgaag aagattgccc aggtgactac
    14941 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    15001 accccagacg gagaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    15061 aagtcacaaa atctgtgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    15121 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    15181 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    15241 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    15301 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac acgctcggtg
    15361 cggtaaatag cgtgattcag cgcgcgtctt cgctggtctt tgacagtgtg gaagccaaaa
    15421 aacacgcgac acgtaatcgc gccaatggcg agttgttcta tggacggcgt ggaacgttaa
    15481 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgtgtgctat
    15541 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    15601 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    15661 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    15721 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggttccatc accatggaag
    15781 tccacgatgt tccggcgatt gttgccgccg tacgcagtgt ggtcccggat gccatcatta
    15841 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    15901 tttctattca ggccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    15961 cggtgtgcaa tgcccgttgc tgggagcagc tgcgagagaa tgcctatctg atgggccaga
    16021 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    16081 tgcgccaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccccaag
    16141 tagcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    16201 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag
    16261 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    16321 atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggca
    16381 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    16441 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    16501 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    16561 ggacgattca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    16621 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    16681 gaccctcata ttgttagcgt tgtttacttt gttatgtttg ccacgctgtt tttagaaaac
    16741 ggcttgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    16801 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    16861 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    16921 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    16981 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    17041 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagc
    17101 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    17161 gttaaacgac atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    17221 accgctggct tgttgggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    17281 cgattttccc cgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    17341 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    17401 ttgcgatgcg ctttcatagc cgacgcgcat cgccgccgcg ctggccttca tgccgtcgtg
    17461 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    17521 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    17581 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcgact
    17641 aatcaggctg aagtgagtct ggcggctgac cagtgccagt aacgcgccgc cgcaaggtcc
    17701 ggtcagcacg tagtacagaa tttcgcggat aatctgtttg ccgagaatgc gcgcatccag
    17761 cggtcgctcc atcacatcca gtaaccgctc tgccgcgcat aaaatctctt ctgataacgt
    17821 ggcggagtta ataccgctgg ctgccattga cggctgaaaa tgttcatctt cgccaatgtc
    17881 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    17941 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca ggagatattc
    18001 attagcgtca taacgaaaca cgcgttcatt gatataaccg attttatgtc cggaaaagag
    18061 aattatgatg ccaggctcgt acatcaccgg tgtgcgtgcg aaaggcgtct cgccatacaa
    18121 caaacgcaca tcgggcaaca gctctgacaa actattttct ttatttttca gtttattaac
    18181 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    18241 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    18301 gcaagacatt ggcagaaatg agcattgaga ggtacggcgc tggcgaccac aatgaaaaac
    18361 atcaggcaga ccgatctctg ccctcatatt gacccagcaa agggagcaag taatgaacaa
    18421 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    18481 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggcggcggca gcgtgaaaaa
    18541 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    18601 cggtattgaa ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    18661 acaaaaagtg acttttctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    18721 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    18781 cggcaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    18841 ttcagaatcc aacgcaggtg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    18901 ccattctgcc catgttcagc ccgtatttgc cgtgctcgat ccggtttata cctacaccct
    18961 gccgtcgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    19021 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    19081 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    19141 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatc ggcgctggcg taccgcagga
    19201 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    19261 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgatacca agcgcgctaa
    19321 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttcagacg atgagcgtat
    19381 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    19441 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    19501 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    19561 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    19621 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    19681 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    19741 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    19801 ggttatcgct cgattgatac cgccgcagcc tacaagaacg aagaaggtgt cggcaaagcc
    19861 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    19921 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    19981 atcgacctct atttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    20041 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    20101 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    20161 atcgaacttc acccgctgat gcaacaacgc cagctacatg cctggaacgc gacacacaaa
    20221 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    20281 gtcattcgcg atctggcaga taaatacggc aaaacccctg cgcagattgt tatccgctgg
    20341 catctggata gcggcctggt ggtgatccca aaatcggtca caccttcacg cattgtcgaa
    20401 aactttgatg tctgggattt ccgtctcgac aaagacgaac ttggcgaaat tgcaaaactc
    20461 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaacg tgcaaattat
    20521 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    20581 ccgcccgttg ctactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    20641 gccggaagct cttaaaagaa caacccatct atatccggga taaacgcaac ggagaggtga
    20701 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    20761 acagcccccc tcacagaccg tagaacaaac agtccggcat atttatcaga actataaatc
    20821 agatgccact gccccttatt ttggtgaaac cggagagcga gcgataactt ctgcgcgtat
    20881 tcaacaggcg cttatcctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    20941 ttatgatccg gtttgtgatt gtcaggattt tggcaatctg gtgttagaaa gcgtagcgat
    21001 aacccaaact gacgccgatc atgccgatgc cgctgtgcgc tttcgtatct ttcaagacga
    21061 taaagaaaag acctcgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    21121 cgatattgtc agcaatcatg gcagcgtctt acaagcggtt aatagcgaga atgaaaaaac
    21181 gctggccact atagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    21241 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    21301 ggcggtcaat gccttctata aaaccacctt caagacggcc aacaatcccg atgaagatat
    21361 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgttatt
    21421 ttcccgcgtt gatgaaattc gagtcctgga gaaaaccacc gattccgccc gcattcatgt
    21481 tcgctttacg ctgaccaatg gcaacaacga agagcaagaa ctgattttac agcggcgcga
    21541 aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    21601 tgaggcaaaa accgccgcca gattaaaaca atgagctgaa ttaaataaca attagccgga
    21661 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    21721 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat
    21781 gtgcaaacat taaatgcaaa aatcgcccgg cttgagcagg atatgaaagc attacgccca
    21841 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgcacaggac
    21901 tatttcgact gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    21961 cattctgtta ccggggatat tttttaacgt ccaaccgccg ctttagggcg tttcttcgca
    22021 ccagcattct gcaggcgaga ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga
    22081 cgctgggtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg gttctggcga
    22141 cgagcctcac gcatctcttc aatagttggc gcaggcacta agcaatcgcg acggctgcca
    22201 atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca gtttgccgga
    22261 tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt cggcacgaag
    22321 acgtcttcac tcttataacc aatcttcgcc agcgggtttt tgccggtgta atacatggtg
    22381 gtcgagttag ccagcggcga cggatagaag ttctgcacct ggtcgaggcg gaagcgatgc
    22441 tttttcagcc atagcgccag attcaccata tcttcatcac gcgtacccgg gtgcgcggag
    22501 atgaaatacg ggatcagata ctgttcttta cctgcctgtt tcgagtaagt atcgaacagc
    22561 tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgataa cggcccttct
    22621 tcggtatgtt ccggggcaat cttcagataa ccgccaacgt gatgggtcgc cagctctttg
    22681 atatagcgcg gatcttctac agcgatgtca taacgcacac cagaggcaat caggatcttt
    22741 ttaatgcctt tcagatcacg cgcacggcga tagagattga tggtcggttc atgattagtg
    22801 tccatgtgcg aacaaatatc cggataaacg cacgacaaac gacggcaggt ttgctcagcg
    22861 cgtggcgatt tgcagcgcaa catatacatg ttggcagtag gcccaccgag atcggaaatc
    22921 acgcccgtaa aacctggaac agtgtcgcgg atcgcttcga tctcattaat aatcgaatct
    22981 tcggaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa agagcagccg
    23041 ccaaagcagc cacgcataat gttgaccgag aaacgaatca tttcgtaagc cggaattcgg
    23101 gcattaccgt aagccggatg cggcacgcgc ttgtacggca gcgcaaagac gctatccatc
    23161 tcttcggtag aaagcggaat agcaggcggg ttgatccata catagcggtc gccatgtttt
    23221 tgcatcaatg cgcgggcgca gcctgggtta gtttcgtggt gcagaatgcg cgaagcatgg
    23281 gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaacac gtaggttttt
    23341 tcccacggtt tcgggcgtgg tggctgcacg gttacagatt tggcttcctg ctttttcgga
    23401 gctaccggtt tgttatccgc gcacggcaaa tcttcgccat acggatgcgg gattgggtcg
    23461 attttcccag gggtatcaag acgggtggaa tccacaccgc tccagcctgg caacgcctct
    23521 tttacgataa tcgcggtatt acgcacatcg cggatttcac taatcggctc gcccatcgcc
    23581 agacggtgcg ccacttccac cagcggacgc tcaccgttac caaacatcag catgtcggct
    23641 ttcgaatcca ccagcacgga acggcgcacg gtatccgacc agtaatcata gtgcgcggta
    23701 cggcgcaggc tggcctcaat accgccgagg atcaccggca catctttcca cgcctcttta
    23761 caacgttggg tataaaccag tgtggcgcga tccggacgct tacccgcgac attatctggc
    23821 gtgtaggcat cgtcatgacg taaacggcga tcggcggtat agcggttgat catcgagtcc
    23881 atgttgccag cagtaacacc gaaaaacaga ttcggtttac ccagacgcat aaagtcgtct
    23941 ttgctgctcc agtctggctg ggcgatgatc ccgacgcgaa agccctgcgc ttccagcata
    24001 cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc agtaaccaaa
    24061 atgatgtcgc agctatccca gccaagttga tccatctctt cacgtgacat cggcaaaaac
    24121 ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc gcgatccggt
    24181 tggatcaggg agatagagct cattttgctt ccagaaatgg taaaaaaata atcaaaggcc
    24241 ggggattata cgctgtatca cgcgcggaaa tgaagtgatg ttgatgaacg gtggcgaaat
    24301 accctggtga ggtttttcac tgcccgactt gggcaagcac atttaaggct ggcggtatcg
    24361 tggcaactgc cgctactatt tatgccgatc agcaacgttc aaatcatatt gcacgacgat
    24421 cggctatccg aggacaccca cagtaaggca aggtatggaa caggttatta caaagcgccg
    24481 ctattacgat attggtctac agattgaaga gctgctgtat tcaggcgtat tcaaagccgg
    24541 agaacgctta ccttcagaac gtgaactgag cgagcggttt aataccagcc gaactaccat
    24601 tcgtgaagcc atcattatgc tggaactgaa aggcgtattg aatgtgaagc aagggtcagg
    24661 gatttttttt gtcgactcca ccgataagct caaccagaaa tcattaatgc cctattcaga
    24721 aattggcccg tttgaactgt tgcaggcgcg tcaggtgatt gagagcaata ttactggttt
    24781 tgcagccagc cagattagct ttaacgagtt acaagagtta aagaaaatca ttagtttgca
    24841 ggaaaacgct atcgctgcgg aaagtgatag atttgaagac ctggatcatc gttttcatag
    24901 cattattgcc gaagcgacgc aaaatcgggt actcatcaaa caagccgctg aattatggcg
    24961 ggcagtgcgt acagaaaacc cccgctggaa gaaacttaac tacaagtacc tgcatgaaaa
    25021 gcatttgcgt ctgcaatggc tggaagatca ccgggctatt ttcctggctc tgcaacagaa
    25081 agattcagag ctcgccaggg aagcgtcctg gagacatctg gaaaacagca aaaatgaact
    25141 gatcaaaatc ttcaaacaag acgcttccat cagcgatttt gatgactttt tcttcgctcg
    25201 ttaaccttca ttagtccaga gctggcggga aaaataacgc cggctctgcc tttcgccctc
    25261 cactggtaca cgctctcttt ttaattggtc taataaatcg actatcatca gatatcttag
    25321 attagcggca attatcctaa aaactgacct ctaaaaaact ttcctttcat tttattcata
    25381 taactcattg atacagatat aaataacgcc ttgttgttct gtttcgtact tttacctttc
    25441 tctgcaagcg tgatgcatct ctctgttttt ggtctagtga attatgtaaa ttggtctaca
    25501 cacaagtcgt caggagtaag agcataatgt cagggaacga aagtatcgct gccgcccact
    25561 atgatcaggt aaccacctgg cctcgggacg ggttacaggc agatattgtc catattggtt
    25621 ttggcgcttt tcatcgcgga caccaggctg tctacacgga tctcactaat caactttcgg
    25681 acacccgctg ggggatcttt gagatcaacc tgtttggtga tgctcaactg atcgaaaact
    25741 taaatgcgca aaatgggctg ttttcggttg tggaaacatc tgcatcgcaa tctacctcac
    25801 gccttgtgcg ttcggtggct ggcggtattc atacccccag agatggcatt gccgcagcaa
    25861 tccataaact ggctgaacct caggtaaaaa tcgtttcatt aaccatcacc gagaaaggtt
    25921 attgcctcga tccgcaaaca cgttcacttg atctcaccaa tggattaatc caacacgatc
    25981 tacaaaaccc ggatgcgcct ctgtcagcta ttggcgtgat cgtctgtgct ttgcaacaac
    26041 gtaaagcggc aggactcgcc gcttttagtg tgctctcctg tgacaacttg ccagacaatg
    26101 ggcatctgac gcgcaatgcc gttctcggtt ttgcccgaca actggaccag cccctggctc
    26161 agtggatcga agaaaatgtc tcatttccag gcacgatggt agatcgcatt gttccggcaa
    26221 tgactgaatc gcaattcgcc ttactggaaa cgaaaaccgg ttatgccgat ccctgcggga
    26281 tcgtctgcga atcatttcgt cagtgggtga tcgaagataa ttttgtgcgc ggacgaccgg
    26341 aatgggataa agccggcgcg atgtttgtca gcaatgttca gccttatgaa gagatgaagt
    26401 tacgcatgtt aaatggtagc cattcatttc tggcttataa cggctctttg gcaggctatg
    26461 agtttatctg gcaatgcatg gaagacgcta attttcgttc cattacccac caactgatga
    26521 ttaatgaaca agcccgaaca cttaatccag acttaaatat caacatccag gaatacgccg
    26581 acctgttaat tgaacgcttt agcaaccgta acgttgcgca ccgtaccggg caaatagcca
    26641 tggacggttc acaaaagctt ccccagcgtg cactgacgcc ctggctgaaa ttacatcagc
    26701 aaaagcaaaa caatgctgtt ctgtcactgc tcgttgctgg ttggttgcat tatgtcattg
    26761 atgttgttga gaaaagccag tctgtcgctg atccaatgaa tgaccaattt caggcgctaa
    26821 taaaggaaca acaagacgca tggcaacagg cgctcgcatt actgcacctt agcgccatat
    26881 ttggtgattt aagcaaccat cagccattta taaatgaaat aaaaatcgcc tttgcaaata
    26941 taaaaaacaa aggcatcaag gccaccatca gccaattatt atcggatgag cagaaatgaa
    27001 aacattaatt tgtcagcagc ctggcgttat ggaatatgtg gaaaaggata ttcccacacc
    27061 agcagataat gaagtgctgt taaaaatcaa agctgtgggt atttgtggta ctgatattca
    27121 cgcttttgcc ggcagacagc ctttttttag ctacccacgt gtattaggtc atgaaatatg
    27181 cgccgaagcg gtttcgcgag gcagccagtg ccaaacagca caatcaggcc agcgctattc
    27241 cgtcatccca tgcattccat gtggcgagtg cgcagcctgt cgggaaaaga aaacgaactg
    27301 ctgcgaacgt gtttcgctgt atggcgtgca tcaggatggg ggctttagtg agtaccttgc
    27361 ggtacgtgaa gacaaccttg tgcctctccc tgacgaggtc agcgacagtg ccggagcatt
    27421 ggttgaatgt ttcgccattg gtgcacatgc cgttcgtcgg gcagagatca aggctgaaca
    27481 aaacgtactg gtgattggcg ctgggccaat cggtctggct accgcagcca tcgccagggc
    27541 taaaggggcg catgttgttg ttgctgatat tgactgtcaa cgtcgccagc acgttgtgga
    27601 tcatctggca attaatgtct tcgacccaac acaggaaggt tttattgccg cgcttagtga
    27661 agtatttggc ggcgaactgg cttgcgtagt cctggatgcg acgggaaata aagcttcaat
    27721 gagtcatgat gtaaatctta ttcgtcatgg cggcaaaatt gttttcatcg gtttgtacat
    27781 tggtgaactt gttattgacg atccgacctt ccataaaaaa gagacaacgt tactcagcag
    27841 ccgcaatgcc acacgggaag attttgcgtt ggtgattgaa ctgatgcgca gcaataaaat
    27901 tcacgaaaat ttaatgaaaa accaggcgtt caatttcttt agtgttggcg aagattacca
    27961 gcgtaacgtt gtagaaaata aaaatatggt caagggtgtg atcacttttt aagggaacat
    28021 atatgacaac tgtttatgtc agtgaagaaa atttaaaatc actggtgcac cacaaattac
    28081 ataccgccgg gctggatgcc gacactgcac aacaggtgac agatgtttta atacatgccg
    28141 atattacagg tgtgcattca catggagtta tgcgtgtcga acattattgt acccgccttg
    28201 ctgccggagg gttaaataaa gccccgcagt ttagtattga gcaaatttca ccgtcagtgg
    28261 ccattctcga ctctgacgac ggaatggggc attcggcatt aattagcgcc actgagcaca
    28321 ctattaaact ggccctggaa gagggtctcg gttttgttag cgttaaaaat acgtctcact
    28381 gtggcgcgtt atcttatttt gcagagatgg tcaccaacaa agggttggtt gctatcgtaa
    28441 tgacgcaaac cgatacctgc gtggctcccc atggcggcgc cgagcgcttt ttgggtacta
    28501 accccatcgc ctttggtttc ccggtggaaa acagccatcc gatgattgtt gatatggcaa
    28561 ccagtgccac ggcttttggc aaaatacttc atgcaaagga aacaggaaaa catattggtg
    28621 aagggctggc gatagataaa gacggttatg gcaccactga tccgcataag attgaaaacc
    28681 tgctaccttt cggccaacac aaaggttcag gcattgcgct ggctattgat gcactgactg
    28741 gcatgctgat gaatgcgaat tttggcaacc atattgttcg catgtatggt gattatgaca
    28801 agatgcgtaa gctggcaagt ttggttattg ccatcgatcc gaaaaagctc ggcaatcctg
    28861 tttttgcaaa aaccatggcg caaatggtca cggagttgca tgccgttaaa ccggctcccg
    28921 gtatcgaaaa agtgttagcg ccgaacgatc cgcaaatgca ctacaaagaa aaatgccaac
    28981 aggaaggtat tccggttcca gcaggaatat tccattatct ggcagagaat taatcactac
    29041 tttttattat aacgatagaa aagcgctgta tttatcgttt ctgttatatt aaccaggagc
    29101 aggatatgaa agcacttcgt cctctgacgg catcgcttat gctgttaacc a
//
LOCUS       DACADA010000048        28932 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.049, whole genome shotgun sequence.
ACCESSION   DACADA010000048 DACADA010000000
VERSION     DACADA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.049"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            74..316
                     /locus_tag="ISB17_002059"
     CDS             74..316
                     /locus_tag="ISB17_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979992.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            431..1069
                     /locus_tag="ISB17_002060"
     CDS             431..1069
                     /locus_tag="ISB17_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAP1979993.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1066..1803
                     /locus_tag="ISB17_002061"
     CDS             1066..1803
                     /locus_tag="ISB17_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAP1979994.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1803..3899
                     /locus_tag="ISB17_002062"
     CDS             1803..3899
                     /locus_tag="ISB17_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAP1979995.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3946..4224)
                     /locus_tag="ISB17_002063"
     CDS             complement(3946..4224)
                     /locus_tag="ISB17_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1979996.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4439..4849
                     /gene="psiE"
                     /locus_tag="ISB17_002064"
     CDS             4439..4849
                     /gene="psiE"
                     /locus_tag="ISB17_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAP1979997.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4943..5833)
                     /gene="malG"
                     /locus_tag="ISB17_002065"
     CDS             complement(4943..5833)
                     /gene="malG"
                     /locus_tag="ISB17_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAP1979998.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5848..7392)
                     /gene="malF"
                     /locus_tag="ISB17_002066"
     CDS             complement(5848..7392)
                     /gene="malF"
                     /locus_tag="ISB17_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAP1979999.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7546..8736)
                     /gene="malE"
                     /locus_tag="ISB17_002067"
     CDS             complement(7546..8736)
                     /gene="malE"
                     /locus_tag="ISB17_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAP1980000.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9101..10216
                     /gene="malK"
                     /locus_tag="ISB17_002068"
     CDS             9101..10216
                     /gene="malK"
                     /locus_tag="ISB17_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAP1980001.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10288..11628
                     /gene="lamB"
                     /locus_tag="ISB17_002069"
     CDS             10288..11628
                     /gene="lamB"
                     /locus_tag="ISB17_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAP1980002.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11861..12781
                     /gene="malM"
                     /locus_tag="ISB17_002070"
     CDS             11861..12781
                     /gene="malM"
                     /locus_tag="ISB17_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAP1980003.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            12960..13457
                     /gene="ubiC"
                     /locus_tag="ISB17_002071"
     CDS             12960..13457
                     /gene="ubiC"
                     /locus_tag="ISB17_002071"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAP1980004.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13470..14342
                     /gene="ubiA"
                     /locus_tag="ISB17_002072"
     CDS             13470..14342
                     /gene="ubiA"
                     /locus_tag="ISB17_002072"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAP1980005.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14497..16920)
                     /gene="plsB"
                     /locus_tag="ISB17_002073"
     CDS             complement(14497..16920)
                     /gene="plsB"
                     /locus_tag="ISB17_002073"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAP1980006.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTKQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17091..17459
                     /locus_tag="ISB17_002074"
     CDS             17091..17459
                     /locus_tag="ISB17_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP1980007.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17569..18177
                     /gene="lexA"
                     /locus_tag="ISB17_002075"
     CDS             17569..18177
                     /gene="lexA"
                     /locus_tag="ISB17_002075"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAP1980008.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18196..19575
                     /gene="dinF"
                     /locus_tag="ISB17_002076"
     CDS             18196..19575
                     /gene="dinF"
                     /locus_tag="ISB17_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAP1980009.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNDTWFVAT"
     gene            19691..19900
                     /locus_tag="ISB17_002077"
     CDS             19691..19900
                     /locus_tag="ISB17_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP1980010.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(19942..20457)
                     /gene="zur"
                     /locus_tag="ISB17_002078"
     CDS             complement(19942..20457)
                     /gene="zur"
                     /locus_tag="ISB17_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAP1980011.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20613..21608
                     /gene="dusA"
                     /locus_tag="ISB17_002079"
     CDS             20613..21608
                     /gene="dusA"
                     /locus_tag="ISB17_002079"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAP1980012.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21742..21984
                     /gene="pspG"
                     /locus_tag="ISB17_002080"
     CDS             21742..21984
                     /gene="pspG"
                     /locus_tag="ISB17_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAP1980013.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(22149..23132)
                     /locus_tag="ISB17_002081"
     CDS             complement(22149..23132)
                     /locus_tag="ISB17_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAP1980014.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(23238..25274)
                     /locus_tag="ISB17_002082"
     CDS             complement(23238..25274)
                     /locus_tag="ISB17_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP1980015.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            complement(25285..26565)
                     /locus_tag="ISB17_002083"
     CDS             complement(25285..26565)
                     /locus_tag="ISB17_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP1980016.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            complement(26592..27371)
                     /locus_tag="ISB17_002084"
     CDS             complement(26592..27371)
                     /locus_tag="ISB17_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAP1980017.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            complement(27364..28917)
                     /locus_tag="ISB17_002085"
     CDS             complement(27364..28917)
                     /locus_tag="ISB17_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP1980018.1"
                     /translation="MTAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERFQRAGHP
                     QNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQMINDNAI
                     PAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSLVERVRF
                     ANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGGIIIAQV
                     KRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSAFRYMEH
                     GPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAIGGVPLL
                     EFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPARPHVTAG
                     CGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVTFSGKRA
                     IRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDLKLMNPL
                     YFQQGSHHE"
BASE COUNT         6843 a         7410 c         7751 g         6928 t
ORIGIN      
        1 ttagttgtgt atactcgatc ccgcccgaaa tgttttgggt aaatctccat tcattcaatg
       61 aagggaaatt gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc
      121 gacttctgcg tgggctgcac ctgtacaggt gggcgaagcg gcaggatcgg cagcaacgtc
      181 ggtttcggcg ggtagttcct ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt
      241 cgcgctcgcg gcaaccggtg gcggtgatgg ttctaatacc gggaccacca caaccacgac
      301 caccagtacc cagtaataaa gtatgtatcc ccaaaataat tcgagtcatc gcatctgaag
      361 cttgaagtat gaaggggatt aaccataacc acactccggt gtggttattc tgcccctctg
      421 gagaagagtc gtgaagcgac cagcactcat tcttatctgc ctgctattac aggcctgttc
      481 agccacgact aaagagctgg gcaattcact gtgggacagt ctgttcggta cgccaggcgt
      541 acagctgacg gacgatgata ttcaaaatat gccctacgcc agccagtaca tgcagcttaa
      601 tggcgggccg cagttatttg tggtgctggc cttcgctgaa gacggacaac aaaaatgggt
      661 cactcaggat caggccaccc ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg
      721 cggcgacaac ctgattgaag tgaataacct tgccgccgac ccgctgatta aacccgcaca
      781 aattgttgat ggtgcaacct ggacgcgcac gatgggctgg accgagtacc agcaggtacg
      841 ctacgccacc gcacgctcag tcttcaaatg ggatggcacc gataccgtca aagtcggcag
      901 cgatgaaacc ccggttcgcg tgctggacga agaagtctcc accgaccagg cgcgctggca
      961 taaccgctac tggatcgaca gcgaagggca aattcgccag tcggaacagt atctcggcgc
     1021 ggattacttc ccggtgaaaa ccaccctcat caaggcggca aaacaatgat taaacaaact
     1081 attgtcgcat tgattttgag tgtgggggcg tcatcggtct tcgcggcggg aaccgtcaag
     1141 gtattcagca atggcagcag tgatgccaaa acgctgacgg gtgcagagca tttaatcgat
     1201 ctggtaggcc aaccgcggct ggcaaacagc tggtggcctg gtgcggtgat tagcgaagag
     1261 ctggccacgg cggcagcatt gcgtcagcag caggcgttga tgacacggat ggcagaactg
     1321 gccgcagact ccagcgccga cgatgccgcc gcaattaacg ccttacgcca gcaaattcag
     1381 gcgttgaaag tgacgggcag gcaaaaaatc aatcttgatc ctgatatcgt ccgcgttgcc
     1441 gaacgcggta acccgccgtt gcagggcaac tacacgctgt gggtcggacc gccgccgtcc
     1501 acggtcacgt tgttcgggct tatcagtcgt cctggcaagc agtcattcac tccaggtcgc
     1561 gacgtggcga gctatctctc tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg
     1621 tgggtggttt acccggacgg acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt
     1681 cacgtagagc cgatgcccgg cagcattatt tatgttggcc tcgcggactc cgtctggagt
     1741 gagacacctg atgcccttaa cgccgacatt cttcagactc tgacgcagcg gataccccaa
     1801 taatgaaaaa aagacatctg cttagcttac tggcgctggg tattagcaca gcttgctacg
     1861 gcgaaacata tcctgcgccc attggtccgt cgcaatcgga tttcggtggc gtaggattat
     1921 tacaaacgcc caccgcgcgc atggcgcggg aaggggagct gagcctgaac tatcgcgata
     1981 acgatcagta ccgttattac tcagcttcag tgcaactctt cccgtggctg gaaacaacgc
     2041 tgcgctacac cgacgtgcgc acccggcagt acagcagcgt cgaagcgttc tctggcgatc
     2101 aaacgtataa agataaagcc ttcgatctca aactgcgttt gtgggaagag agttattggc
     2161 tgccgcaagt ggcggttggt gcgcgggata tcggcggtac ggggctgttt gatgcggaat
     2221 atcttgttgc cagcaaagcc tgggggccgt tcgattttac actcggcctg ggctggggat
     2281 atctgggcac cagcggtaac gtgaaaaatc cgctctgttc agccagtgat aaatattgct
     2341 atcgcgataa cagctacaaa caggcgggat ctatcgacgg cagccagatg ttccacggcc
     2401 cggcctcact gtttggcggc gtggaatacc agacgccctg gcaaccgctg cgtctgaaac
     2461 tggagtatga aggcaataat tatcagcagg attttgccgg aaagctggag caaaaaagta
     2521 agtttaacgt cggggcgatt tatcgcgtta ccgattgggc cgacgttaac cttagctatg
     2581 aacgtggcaa cacttttatg tttggcgtca cgctgcgcac caactttaac gatctgcgcc
     2641 cgtcttacaa cgataacgcc cgcccgcaat atcaaccgca gccgcaggat gccattttgc
     2701 agcattcggt ggtggcgaat cagttaacgc tgttgaaata caatgccgga cttgccgatc
     2761 cgcagatcca ggcgaaaggc gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg
     2821 atccgcgcga agggatcatc cgtgctaatc ggatcgtgat gaacgatctg ccggatggga
     2881 taaaaacgat ccgcattacg gaaaatcgcc ttaatatgcc acaggtgacg acggaaaccg
     2941 atgtcgccag cctgaaaaat catctcgccg gagagccgtt gggccacgaa acgaagctgg
     3001 cgcaaaaacg cgtcgagcca gtggttccga agtccaccga gcagggctgg tatatcgaca
     3061 aatcacgctt tgatttccat atcgatccgg tgctgaacca gtcgatcggt ggcccggaaa
     3121 acttttacat gtatcagctg ggcgtgatgg gaacggcgga tttgtggctg acggaccatc
     3181 tgctgaccac tggcagcctg tttgctaacc ttgccaacaa ctacgacaag tttaactaca
     3241 ccaatccacc gcaggactcg cacttaccgc gcgtgcgtac ccatgtgcgc gagtatgtgc
     3301 agaatgatgt ctatgtgaat aacctgcaag ccaactactt ccagcatctg ggcaacggct
     3361 tctacggtca ggtctacgga ggttatctcg aaaccatgtt tggcggcgtg ggggcagaag
     3421 tgttgtatcg cccgctggat agcaactggg cgtttggtct ggatgccaac tacgttaaac
     3481 agcgcgactg gcgtagcgca aaagatatga tgaaattcac cgattacagc gtgaaaaccg
     3541 gacatctgac cgcgtactgg acgccatctt tcgcccagga cgtgttagtg aaagcgagcg
     3601 tcgggcagta tctggctggg gataaaggcg gcacgctgga gatcgctaaa cgctttgaca
     3661 gcggcgtggt ggttggcggc tatgccacga tcacgaatgt ttcgaaagag gagtacggcg
     3721 aaggggactt caccaaaggc gtgtatgtct ctgtaccgct ggatctcttc tcgtctggcc
     3781 cgacacgcag ccgtgcggcg attggctgga cgccgctgac gcgtgacggt ggtcagcaac
     3841 ttgggcgtaa gttccagttg tatgacatga ccagcgaccg tagcgtcaat ttccgctaag
     3901 tcatgggaaa ggtgccaggt ttcgcactca ctggcacctt tattcttaat ggcctgtcga
     3961 gccatcagga acgcagttca cccccggttt gcgatctggt gtgtaaaggc gaaaacattc
     4021 tgtcttcctg tccgtcgata tcggttgcag gtctggttgc ctgagcgact gggccatcat
     4081 tgggctggag acattgcttt gcggtaggtc ggctttatca gcagtaccga gcggaccagc
     4141 ataagcagga agaacagaga ctgataacat caaagcagca aaataaggct tcatttttac
     4201 cacctttatc aggttacgtt tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt
     4261 gctggtggct gggaatcacc tgaatgggtg atttttttgg gctgtgtgtt ttgccagatg
     4321 agacattgat gggagatgcc cacttaattg agctgaacaa aacatacaca aaaaatatag
     4381 atctccgtca catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat
     4441 gacgtcactc tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa
     4501 tcttggcctg ttgtgcctcg gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca
     4561 tctggctgat gtgctgttcg cgccagaaca aaccagtaaa tatgagctgg tagaaggtct
     4621 ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg
     4681 ttttcacttc ccgctacgtt actttgtcta tattgggatc actgccattg tgcgcttgat
     4741 catcgtcgat cataaatcgc cactggatgt gttgatctac tcggcagcga tcctgttact
     4801 ggtgatcacc ctgtggttgt gtaattcgaa acggctgaag cgggagtaaa aaaaggcgct
     4861 ccgtggagcg ccgaataaca gtcacaagtt gggataacgt aagttgaggg tgcagcggca
     4921 taacattggc agaacaacat ctttaacctt tcacaccacc tgccgtcagg ccgttgacca
     4981 gccagcgttg agccagcaag aagacgatgg tgatcggtaa tgcagacatc acggcagcgg
     5041 cggcaaagtc accccacagg tagttttgcg ggttgaggta ttgctgcatc cccacggcca
     5101 gggtgtagct gtttacgtca cgcagtaata gcgacgcgac cggaacttca gtaatggcag
     5161 cgataaacga caggatgaac acaaccgcca gaatcggtac tgacagcggc aacaggacaa
     5221 ggcggaaggc ctgccacggt gtcgcaccat ccagcgccgc cgcttcttcc agcgaactgt
     5281 cgatggtttc gaaataacct ttgatggtcc agacatgcag cgcaatcccg cccagatacg
     5341 cgaaaattac gccgccgtga gtattcaggc caatgaatgg aatgtactca cccagacgat
     5401 caaacaacgc atacaacgcg accagtgaaa gtactgccgg gaacatctgg aaaatcagca
     5461 ttcctttcag cagcgtcgct ttgcctggaa agcgcatacg ggcgaaagcg taggcgcagg
     5521 tggtggagag cgccacaatg ccaatcgcgg aaatcccggc gacctttacc gagttccaca
     5581 gccacagcag taccgggaat ggcggtggcg taatgcgacc atcagcctgt tccacgctaa
     5641 aacctaacgc cagtttccag tgatcccagg agatttgctc cgggatcaga ctaccggtcg
     5701 cgaagttccc ctgacgcagc gagatggcga cgaccatcag cagcgggaac ataattgccg
     5761 cgataaaaag tagcagtagc aggtgagtaa taaacaaacg tgctttttgc gatttcggtt
     5821 ggaccattgc catttttgtt atctccctta atcaaacttc attcgcgtgg ctttcaggtt
     5881 cactatcgcc agcgcgccca ccagcaggaa gatcagcgtg gcaattgctg ctgccagacc
     5941 gaagtcctga cccccgccgc cttcaaaagc gatacggtag gtgtagttaa caagcaggtc
     6001 ggtataaccg gctggcgtgg tcgtgccaag acgatccggg ccgccgttgg ttaacagttg
     6061 aatcagcacg aagttgttaa agttaaaggc gaagctggcg atcatcagcg gcgtcagcgg
     6121 tttaatcagc agcggcagcg taatcttaaa gaagttctgg aatggacctg cgccatccat
     6181 tgctgaggct tcatacaaat cgtccggaat cgctttcagc aagcccatgc agaggatcat
     6241 catgtacgga taacccagcc aggtgttgac gataatcagc atcgtgcggg cggtggtcgg
     6301 atcgctgaac caggcgggct tcacgccaaa cagcgcgctc aacatcatgt tgatttcacc
     6361 aaaactctgg ttaaacaacc ctttgaaaat caagattgaa atgaacgatg gcaccgcgta
     6421 gggcagaatc agcaggacgc gatagaccgc tttgccgcgc aacgcttccc actgcaccag
     6481 acacgccaga accatgccga ccgccaccgt taaaaagaca gtgatcagcg agaacaccac
     6541 ggtccagacg aaaatggcga ggaacggttt ctgaatgcct tcgtcggtaa agacgcgggt
     6601 aaagtttttc cagccggtgg tcacggtgta accggggctt agcttttcat caccccagtt
     6661 accgtcggca gtgatggact ggtaaaaacc aatctggtcg tttggacgat attttacgcc
     6721 actctgatta ttggtcagcg tgccgtcacc gtcgagtgtg tagagcggct gcgtgccaga
     6781 aaactggcgc agggagctca tcatcacttt gttgccatcc ggcagaatgg cggtaatgtc
     6841 actcagtgcc tgacgattct gggtaattac gcgcagattc gcgcgttcgc cttcgggctg
     6901 ggcggtcgtt tctttcagtt gcagtttttg ctcgccgcca aatttaaaag cgtcggagag
     6961 gtaatttttg ccggtttcgc cgtcgctgag cgccagttgc cactcatcgc ccgccggata
     7021 aagaccaaaa ttatagattt tgcctgcttg ccaggagcga tctagcaaca cttcctgcgc
     7081 acgttcaaaa gtcagctggt tagtgctgct gtagttggtg aaggcaatgg cgatggtgca
     7141 gaccagaggg aagaggacga ataatcccat tccagccatt cccgggtaaa catagcgcca
     7201 ggcgtaggct ttacgattgg cgaaaatata cagccccgct gagctcaata tcagcgtggt
     7261 aatggcgaac aggtattccc cttgtgcgta cattaaaaca acaaggtaac ccaccagcag
     7321 gccgagcaga cctaacactg accatttcag cgcgtcgctt tgccaccaat gtttcttttt
     7381 aatgacatcc atggggttct tcctcattcc aggacggata aggctttcat accttatccg
     7441 acaacaactg cctgatgcga cgctgacgcg tcttatcagg cctacatacg tttcaatttc
     7501 gtaggccgga taaggcgttc acgccgcatc cggcatttca cagcattact tggtgatacg
     7561 agtctgtgca tctttcagcg cagcatccac agtctgacga ccgctggcgg cgttgatcac
     7621 cgcagtacgc acggcatacc agaaagcgga catctgcggg atgttcggca taatttcacc
     7681 tttctgagcg ttatccatgg tggcggcaat acgtggatct ttcgcaagtt catcctgata
     7741 ggacttcagc gctactgcac ccagcggttt gtctttattg accgcttcca gaccttcgtc
     7801 agtcagcaga tagttttcga ggaactcttt cgccagctct ttgttcggac tggcggcgtt
     7861 aatacctgcg ctcagcacgc caacgaacgg tttagatggt tgacctttga aggtcggcag
     7921 taccgttaca ccataattca ctttactggt gtcgatgttg gaccatgccc acgggccgtt
     7981 gatagtcatc gctgtttcgc ctttattaaa ggcagcttct gcgatggagt aatcggtgtc
     8041 tgcattcatg tgtttgtttt taatcagatc aaccaggaag gtcagacccg ctttcgcgcc
     8101 agcgttatcc acgcctacgt ctttaatgtc gtacttgccg ttttcgtact tgaacgcata
     8161 acccccgtca gccgcaatta gcggccaggt gaagtatggt tcttgcaggt tgaacatcag
     8221 cgcgctctta cctttcgctt tcagttcttt atccagcgcc gggatctctt cccaggtttt
     8281 tggcgggttc ggcagcagat ctttgttata aatcagtgat aacgcttcga cagcgatcgg
     8341 gtaagcaatc aacttgccgt tgtaacgtac ggcatcccag gtaaacggat acagcttgtc
     8401 ctggaacgct ttgtccgggg tgatttcagc caacaggcca gattgagcat agccaccaaa
     8461 gcggtcgtgc gcccagaaga taatgtcagg gccatcgcca gttgccgcaa cctgagggaa
     8521 tttctcttcc agtttatccg gatgctcaac ggtgacttta attccggtat ctgtctcgaa
     8581 tttcttaccg acttcagcga gaccgttata gcctttatcg ccgttaatcc agattaccag
     8641 tttaccttct tcgattttgg cgagagccga ggcggaaaac atcatcgtcg ttaatgcgga
     8701 taatgcgagg atgcgtgcac ctgtttttat tttcataatc tatggtcctt gttggtgaag
     8761 tgctcgtgaa aacacctaaa cggactctag tttctttata cggcaacctc tttccatcct
     8821 ccttgcccct acgccccatc gttgctttgt gtgatctctg ttacagaatt ggcggtaatg
     8881 tggagatgcg cacataaaat cgccacgatt tttgcaagca acatcacgaa attccttaca
     8941 tgacctcggt ttagttcaca gaagccgcgt tctcatcctc ccgcctcctc ccccataaaa
     9001 aagccagggg gtggaggatt taagccatct cctgatgacg catagtcagc ccatcatgaa
     9061 tgttgctgtc gatgacaggt tgttacaaag ggagaagggc atggcgagcg tacagctgca
     9121 aaatgtaacg aaagcctggg gcgaggtcgt ggtatcgaaa gatatcaatc tcgatatcca
     9181 tgaaggtgaa ttcgtggtgt ttgtcggacc gtctggctgc ggtaaatcga ctttactgcg
     9241 catgattgcc gggcttgaga cgatcaccag cggcgacctg ttcatcggtg agaaacggat
     9301 gaatgacact ccgcctgcag aacgcggcgt tggtatggtg tttcagtctt acgcgctcta
     9361 tccccacctg tcagtagcag aaaacatgtc atttggcctg aaactggctg gcgcgaaaaa
     9421 agaagtgatt aaccagcgag ttaaccaggt tgcggaagtg ctgcaactgg cgcatttgct
     9481 ggatcgcaaa ccgaaagcgc tctccggtgg tcagcgtcag cgtgtggcga ttggccgtac
     9541 gctggtggcc gagccaagcg tatttttgct cgatgaaccg ctctccaacc tcgatgctgc
     9601 actgcgtgtg caaatgcgta tcgaaatctc ccgtctgcat aaacgcctgg gccgcacaat
     9661 gatttacgtc acccacgatc aggtcgaagc gatgacgctg gccgacaaaa tcgtggtgct
     9721 ggacgctggt cgcgtggcgc aggttgggaa accgctggag ctgtatcact atccggcaga
     9781 ccgttttgtc gccggattta tcggttcgcc aaagatgaac ttcctgccag taaaagtgac
     9841 cgccaccgca atcgatcaag tgcaggtgga gctgccgatg ccgaatcgtc agcaagtctg
     9901 gctaccggtc gaaagccgtg atgtccaggt gggagccaat atgtcgctgg gtattcgccc
     9961 ggagcatcta ctgccgagtg atatcgctga cgtcatcctt gagggtgaag ttcaggtcgt
    10021 cgagcaactc ggcaacgaaa cgcaaatcca tatccagatc ccttccattc gtcaaaacct
    10081 ggtgtaccgc cagaacgacg tggtgttggt agaagaaggt gccacattcg ctatcggcct
    10141 gccgccagag cgttgccatc tgttccgtga ggatggcact gcatgtcgtc gactgcataa
    10201 ggagccgggc gtttaagcac cccacaaaac acacaaagcc tgtcacaggt gatgtgaaaa
    10261 aagaaaagca atgactcagg agatagaatg atgattactc tgcgcaaact tcctctggcg
    10321 gttgccgtcg cagcgggcgt aatgtctgct caggcaatgg ctgttgattt ccacggctat
    10381 gcacgttccg gtattggctg gacaggtagc ggcggcgaac aacagtgttt ccagactacc
    10441 ggtgctcaaa gtaaataccg tcttggcaac gaatgtgaaa cttatgctga attaaaattg
    10501 ggtcaggaag tgtggaaaga gggcgataag agcttctatt tcgacactaa cgtggcctat
    10561 tccgtcgcgc aacagaatga ctgggaagct accgatccgg ctttccgtga agcaaacgtg
    10621 cagggtaaaa acctgatcga atggctgcca ggctccacca tctgggcagg taagcgcttc
    10681 taccaacgtc atgacgttca tatgatcgac ttctactact gggatatttc tggtcctggt
    10741 gccggtctgg aaaacatcga tgttggcttc ggtaaactct ctctggcagc aacccgctcc
    10801 tctgaagcgg ggggttcttc ctctttcgcc agcaacaata tttatgacta taccaacgaa
    10861 accgcgaacg acgttttcga tgtgcgttta gcgcagatgg aaatcaaccc gggcggcaca
    10921 ttagaactgg gtgtcgacta cggtcgtgcc aacctgcgtg ataactatcg tctggttgat
    10981 ggcgcatcga aagacggctg gttattcact gctgaacata ctcagagtgt cctgaagggc
    11041 tttaacaagt ttgttgttca gtacgctact gactcgatga cctcgcaggg taaaggtctg
    11101 tcgcagggtt ctggcgtcgc gtttgataac gaaaaatttg cctacaatat caataacaac
    11161 ggtcacatgc tgcgtatcct cgaccacggt gcgatctcca tgggcgacaa ctgggacatg
    11221 atgtacgtgg gtatgtacca ggatatcaac tgggataacg acaacggcac caagtggtgg
    11281 accgtcggta ttcgcccgat gtacaagtgg acgccaatca tgagcaccgt gatggaaatc
    11341 ggctacgaca acgtcgaatc ccagcgcacc ggcgacaaga acaatcagta caaaattacc
    11401 ctcgcacaac aatggcaggc tggcgacagc atctggtcac gcccggctat tcgtgtcttc
    11461 gcaacctacg ccaagtggga tgagaaatgg ggttatgact acaccggtag ctcttcaacc
    11521 aacccatact acggcaaggc tgtgtcggct gatttcaatg gcggtagctt cggtcgtggc
    11581 gacagcgacg agtggacctt cggtgctcag atggaaatct ggtggtaata gcaaaaaact
    11641 gggccggata aggtgtttgc accgcatccg gcaatcaatg cctgatgcga cgctgttgcg
    11701 tcttatcagg cctacaaccg ctgccgaaag taggccggat aaggcgttta cgccgcatcc
    11761 ggcataaaaa cagctttaag gggcgaaagc ccctctgatt atcgggttta gcgcgctatt
    11821 gcctggctac cgctgagctc cagattttga ggtgaaaaca atgaaaatga ataaaagtct
    11881 catcgctctc tgtttatcag cagggttgct ggcaagcgca cctggaatca gccttgccga
    11941 tgttaactac gtaccgcaaa acaccagcga cgcgccagcc attccatctg ccgcgctgca
    12001 acaactcacc tggacaccgg tcgatcaatc taaaacccag acgactcaac tgtcgaccgg
    12061 cggccaacaa ctgaacgttt ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc
    12121 aaacattggt gaactgacac tgacgctgac cagcgaagtg aacaaacaaa ccagcgtatt
    12181 tgcgccgaac gtgctgattc ttgatcagaa catgacccca tcagccttct tccccagcag
    12241 ttatttcacc taccaggaac ccggcgtgat gagtgcagat cgtctggaag gcgttatgcg
    12301 cctgacaccg gcactggggc aacagaaact ctatgtactg gtcttcacca cggaaaaaga
    12361 tctccagcag acaacccaac tgctcgaccc ggcaaaagcc tatgccaaag gcgtaggtaa
    12421 ctcggtgccg gatattcctg acccgattgc ccgtcacacc actgatggtt tgctgaaact
    12481 gaaagtgaaa acgaactcca gctccagcgt gttggtagga ccactatttg gttcttccgc
    12541 tccagctccg gttacggtag gtaacacggc ggcaccagct gtggctgcac ccgctccggc
    12601 accggtgaag aaaagcgagc cgatgctcaa cgacacggaa agttatttta ataccgcgat
    12661 caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga
    12721 acgcctgggg tcgacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta
    12781 attacgctcc acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt
    12841 ctcctcctgt actattgttg cgtttttgtt gcgcattagt tcacttaatt tgctttacat
    12901 ctcccgtaaa tatttttctg cgatacaatg cctttacgtt atgtaacgga gagttcggca
    12961 tgtcacaccc cgcgttaacg caactgcgtg cgctgcgcta ttttacagag atccctgccc
    13021 tggagccgca actgctcgac tggctgttgc tggaagattc catgacaaaa cgttttgaac
    13081 agcagggaaa aacggtgagc gtgacgatga tccgcgaagg gtttgtcgag cagaatgaaa
    13141 tccccgaaga actgccactg ctgccgaaag agtctcgtta ctggttacgt gaaattttgt
    13201 tatgtgccga tggtgaaccg tggcttgccg gtcgtaccgt cgttcctgtg tcaacgttaa
    13261 gcgggccgga gctggcgtta caaaaattag gtaaaacgcc gttaggacgc tatctgttca
    13321 catcatcgac attaacccgg gactttattg agataggccg tgatgccgga ctgtgggggc
    13381 gacgttcccg cctgcgatta agcggtaaac cgctgttgct aacagaactg tttttaccgg
    13441 cgtcaccgtt gtactaagag gaaaaaaata tggagtggag tctgacgcag aataagctgc
    13501 tggcgtttca tcgcttaatg cgtacggata agccaattgg cgcgttactg ctgctctggc
    13561 caacattatg ggcgttgtgg gtggcgacac cgggcgttcc ccagctctgg atcctggcgg
    13621 tgtttgtcgc gggcgtctgg ctgatgcgcg ctgctggttg cgtggtgaat gattatgctg
    13681 accgcaagtt tgatggtcat gttaagcgca cggcgaaccg accacttccc agcggcgcgg
    13741 tgacagagaa agaagcgcgt acgctgtttg tagtgctggt actgatttca tttttactgg
    13801 tgctgacgct gaatacgatg actattctgt tgtcaattgc cgcgctggcg ctggcgtggg
    13861 tatacccgtt tatgaagcgg tatacccatc taccgcaagt ggtgctgggc gcggcgtttg
    13921 gctggtcgat tccaatggct tttgccgctg tgagtgagtc ggtgccattg agttgctggt
    13981 taatgttcct cgccaatatt ctctgggcgg tggcttacga cacgcagtat gcgatggttg
    14041 accgcgatga tgatgtgaag attggcatta aatccaccgc aatcctgttc ggccaatacg
    14101 ataaattgat tattggtatt ttgcagattg gcgtactagc gctgatggcg atcatcggtg
    14161 agttaaatgg cttaggctgg ggatattact ggtcaattct ggtagctggc gcgctgtttg
    14221 tttatcaaca aaaactgatt gccaaccgcg agcgtgaagc ctgctttaaa gcatttatga
    14281 ataataacta tgttggtctg gtactgtttt tagggctggc aatgagttac tggcatttct
    14341 gatgatgtaa aaaagccgga tgatcatccg gctttcttct gggttgcctg atgcgctgcg
    14401 cttctcaggc ctacacaaca tatcgcaatt tattgaattt gcagatcatg gtaggccgaa
    14461 taaggcgttt gcgtcgcatc cggcaattct cactgattac ccttcgccct gcgtcgcact
    14521 ctcaatcgtc aaacgcacgt ctgatgtaat caactccgcc agcaactgat ataccttcat
    14581 cgtttctgcc ggttcggcgt cgccgctatc gctgatatac ccttcatcac gcagcgtcag
    14641 caccagagaa ctgaacaccg ccttgtcgaa gaattccggc gcgttgatac cgtgcagcac
    14701 ggagagacgt tgcgcgacgg tgcggctctc tttttccagc gtaccgcggt tgatcgacgg
    14761 gttggcactc aacagccaga aggtgatggc ataacgttgc agcgtttcgc gcgcgcctgc
    14821 ggccagcagc tgtagcgtgc gagaatgcgc cgggttgata tgcaactcat catcttgcag
    14881 ggtaatcagc ccctgacgtt gcatctcatt tgccagcgca tcaataacgt ccggtaactc
    14941 gtcgcgatcc cagcgcagga acagttccgc tttcagcatt gggtaaagca cattgacgtg
    15001 ctccatcaat acgtcgcggg agatgtggcg atgctgggtg acaattgccg ccatcagcga
    15061 aggcagcacc aacatatgcg caatgttgtt gcgatagtag gtcatcagca ccgcttgctc
    15121 gcgcggcaga atgatgatgt cgccgatagt gtctttctcg acttcaaact tgttcatttg
    15181 cagcgcgtga tcgataagct cgctggcgct ggctgaagga acggtagagt ccgtggagta
    15241 aggcacgttg cgcatcagat ccaggtagca gttgagttgc ttggttaact gctcgcgggt
    15301 gagtgagcgc tgacgtgacg ccagaagcgc agtacagcac aggttcatgg cgttcgccgc
    15361 gcctgcgttg ttaatgcgta ccatcagatc ggcagcaata ttattgaccg tcggcgttaa
    15421 ccaggccgga cgcaccgctt cgatgggatc gatagattca cgccagtctg gtacgtgctg
    15481 gttaaggtag gtcatcaacg gcattggttc accgaagttg acgtaaccct gaccgagatt
    15541 acgcagcttg cttaaaccgc gcagcatctg cggcaggctc tctttctctt tcgtcgcacc
    15601 gcgcagttct ttggcgtaag tacccacttc catgacgtgc tcataaccga tatagatcgg
    15661 aatcagcgta atcggacgag tgccgccacg cagcatcgcc tgaatggtca tcgatagcgt
    15721 accggttttc ggatccagca aacgccccgt acgggaacga ccgccttcca cgaaatactc
    15781 gacggaataa ccacggctga acagttcgcc gagatactca cggaaaacgg tggaataaag
    15841 tttattgcct ttaaacgtac ggcgaataaa gaacgccccc agacggcgga aaatcggccc
    15901 ggccggccag aagttcaggt tgatcccggc ggcgatatgc ggcggcacca gcccctggtg
    15961 atacagcacg taagaaagca gcaggtagtc catgtgactg cggtggcaag gcacatacac
    16021 tagctcatgg ccgtcgtggg ccagctggcg aacgcgctcg gcgttatgga cgttgatacc
    16081 ctggtaaagt cggttccagg tgaagcccag aatgcggtca gtcaggcgga tcatctcgta
    16141 agagaaattc gccgcaatct cttccatcag cgcaatcgcg ttctgctgcg ctttttcatg
    16201 ggagattttt ttgctgcgtg cttcatcttc taccgctttc gcaatggcgc gggaggcgag
    16261 cagcttatta aacagatcct gacgagcagg cagacgtgga cccacggcag ccagacgttg
    16321 acgggcaaag tgcatacgcg ccacgcgcgc cagtttctga gcgatagttt tatccgtgcc
    16381 gtgttcatcc gccatacggc gcagtgaaac tgacggcgag aaacgcacaa aactgtcgcg
    16441 accgagccac agtacagcga aaaatttctg tacgccgtta agcatacgca gcggcgggtt
    16501 cacttcgcct ttttcacgcc ccggcgcgcg accaaacatc accgatactg gcaccatctg
    16561 cacatccaga tttgggttgc tgcggtgcaa atcgagatag tcgtggaaca gcttaataga
    16621 ctcttctttc ggcgtgtaat aggtgaacac acgcggcccg ccgtgaatga acacatagcg
    16681 cggcagtagc gtgccgtcga tttccagcgg ttctaacggg tcaggcaggt catgtgccag
    16741 acactgggcg cgcaacgtca gcaagtcagc tttcgagttg tacggtaaaa cgtacataat
    16801 tggacgagag gtatccagcc ccagttccgg ggcaggatcc gccggaatag acttgctttt
    16861 taccaggatg cttaatggta aattcagtaa tttgtagtaa attcgtggcc agccggacat
    16921 aaacgatgta aagcctctgg ttaataatgc aaatgcgcgg caaggatagc agaaagtcat
    16981 gggaaattct gtggtatccg ctcatgtttc gcgcggcgtt acgcaaaccc gaatcatcgg
    17041 atttaacggt acactgatat tgacgctcat aatgtaaaaa ggttctttca atggccaata
    17101 ataccactgg attcatccga attattaaag ctgctggcta ttcctggaaa ggtttacgcg
    17161 ctgcatggat caacgaagcg gcattccgtc aggaaggcgt agcggtattg ttagcggtgg
    17221 ttatcgcctg ctggctggat gtggacgcga ttacccgcgt gctgcttatc agctccgtga
    17281 tgctggtgat gattgtggaa attctcaata gcgccatcga agctgtggtt gaccgaattg
    17341 gctctgaata ccatgagctt tccggacgcg ctaaagatat gggatctgcc gcagtgctga
    17401 ttgccattat cgtcgccgtg attacctggt gcatcctgtt atggtcgcat tttggataac
    17461 ccttccagat ttcgataaat ctctggttta ttgtgcagtt tatggttcca aaatcgcctt
    17521 ttgctgtata tactcacagc ataactgtat atacacccag ggggcggaat gaaagcgtta
    17581 acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca gacaggtatg
    17641 ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa
    17701 gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc
    17761 gggattcgtt tattgcagga agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc
    17821 ggtgaaccgc ttctggcgca acagcatatt gaaggtcatt atcaggtcga tccttccttg
    17881 ttcaagccga atgctgattt cctgctgcgt gtcagcggga tgtcgatgaa agatatcggc
    17941 attatggatg gcgatttgct ggcagtgcat aaaactcagg atgtacgtaa cggtcaggtc
    18001 gttgtcgcac gtattgatga cgaagttacc gttaaacgcc tgaaaaaaca gggcaataaa
    18061 gtcgaactgt tgccagaaaa cagcgagttt aaaccaattg tcgttgacct tcgtcagcag
    18121 agcttcacca ttgaggggct ggcggttggg gttattcgca acggcgactg gctgtaacat
    18181 atctctgaga ccgcgatgcc gcctggcgtc gcggtttgtt tttcatctct cttcatcagg
    18241 cttgtctgca tggcattcct cacttcatct gataaagcac tctggcatct cgccttaccc
    18301 atgattttct ccaatatcac cgttccgttg ctgggactgg tcgatacggc ggtaattggt
    18361 catcttgata gtccggttta tttaggcggc gtggcggttg gtgcaacggc gaccagcttt
    18421 ctctttatgt tgttgctgtt tttacgcatg agcaccaccg ggctgactgc acaggcttat
    18481 ggtgccaaaa atcctcaggc attagcccgt gcgctggtgc aaccgttgct gttggcgttg
    18541 ggggccgggg cgttaattgc gctgctgcgt acgccgatta tcgatctggc gctgcatatt
    18601 gttggtggta gtgaagcagt cctggaacag gcgcgacgct ttcttgaaat ccgctggtta
    18661 agcgcaccgg cgtcgctggc gaatctggta ttacttggct ggttactcgg cgtgcaatat
    18721 gcccgtgcgc cagtaatttt gttagtggtc ggcaatatcc tcaacattgt gctggatgtc
    18781 tggctggtga tggggctgca tatgaacgtg cagggcgcgg ctctggcgac ggttattgcg
    18841 gaatatgcaa cattgctgat tggtctgcta atggtgcgta aaattctcaa actacgcgga
    18901 atctccggcg aaatgctgaa aacagcctgg cgaggaaact tccgtcactt gctggcgctt
    18961 aaccgcgata tcatgctgcg ctcgctgttg ctgcaactct gtttcggcgc gatcactgta
    19021 cttggcgcgc gactggggag tgacattatc gctgttaacg cggttctgat gacgctactc
    19081 acctttaccg cctatgcgct ggatggtttt gcctacgcgg ttgaagcaca ttccggtcag
    19141 gcatacggtg cgcgcgacgg tagtcaattg ctggatgtct ggcgggcagc gtgccgccag
    19201 tcggggatcg tagcgttact gttttcggtg gtttatttgc tggcagggga acacatcatt
    19261 gcgttgctga cgtcgttaac ccagattcag cagctggctg accgctatct tatctggcag
    19321 gtgattttgc cgttggtcgg cgtctggtgt tatctgctgg acggcatgtt tataggcgca
    19381 acgcgtgccg ccgaaatgcg taacagtatg gcggtggccg ccgcaggttt tgcactgacg
    19441 ctccttacgc tgccgtggct ggggaatcat ggtttgtggc tggcgttaac cgtctttctg
    19501 gcgttacgcg ggctttctct ggcggctatc tggcggcgtc actggcgtaa cgatacctgg
    19561 tttgtcgcaa cgtgacggtt aaaaattctg aataaataat cctaagccaa attgctgact
    19621 acacttaatc tcacgttcag aagaaaagtg aacgtactct cattcacaac ctaacgatga
    19681 ggtcttgatt atgaataaag atgaagccgg cggtaactgg aaacagttta aaggtaaagt
    19741 gaaagagcaa tggggcaaac tgaccgatga tgatatgacg atcattgaag gaaaacgtga
    19801 tcagttagtg ggtaaaatcc aggaacgcta cggttatcag aaagatcagg cagaaaaaga
    19861 ggttgatagt tgggaaaaac gccatgatta ccgctggtaa ttaatccctc ctgcccgaca
    19921 tgtacaagga tgtacaccct tttagcgagg tttctttttc acctgaatag agtggtcatg
    19981 atggcactgt tcaggatgac gacacgcttc tacttctaca catgctgaac ataatccatg
    20041 tgcttcaata acattatgcc gcagggcaaa acccattttt gccgccagcg tatgcatgat
    20101 gtcttccacg ccttcagcac attcttcttt caccgcgccg cagcgatcgc aaataaacat
    20161 ggctgaagta tgggtgggct gatcgaacag atgacagagc acataactgt tggtggattc
    20221 caccttatgc acaaaacctt gttcaagcag aaactccagc gcgcgataaa ccgttggcgg
    20281 cttggcttgc ggttcagctt cgcgcagcaa atcaagcaga tcataagcgc tgatagcgcc
    20341 atcctgcaga ctcatcaggc gcaacacttc gagacgctgt ggggtcaggc gcacattacg
    20401 ctgtgcgcag attttttcag cctgcgctaa taactcctgc gttgtggtct tttccattgg
    20461 gcacctcgac agtggggtta agaatgtcct tactttacca tagttctcac ttaaacgccg
    20521 agaacagggt gtcatggctg tcacattgcc tgatcgcata ttcgttatgc tatagtagcg
    20581 cccccaattt accagatgct taaaaagtcg ccatgccagc agaatcacac actgtttatc
    20641 ccgcttaccg tttctcgatt gccccgatgc ttgactggac ggacagacat tgccgttatt
    20701 tcctgcgtct gctttcccgc aatacgttgc tgtataccga aatggtgacc acaggggcga
    20761 ttattcacgg taaaggtgat tacctggcgt acagtgaaga agaacatccg gtagcgttgc
    20821 aactcggggg tagcgatccg gcggcgctgg cacagtgtgc gaagctggca gaagcgcgcg
    20881 gatatgatga gatcaacctg aatgtcggct gcccgtctga ccgggtgcag aacggcatgt
    20941 ttggtgcgtg tctgatgggt aatgcgcagc tggttgccga ctgcgtgaaa gcgatgcgcg
    21001 atgtggtgtc gattccggtg acggtgaaaa cgcgtattgg catcgatgat caggacagct
    21061 atgaatttct ctgcgatttc atcaacaccg tttccggcaa aggtgagtgt gagatgttca
    21121 tcatccacgc acgtaaagcc tggctttctg ggttaagtcc gaaagaaaac cgtgagatcc
    21181 cgccgctcga ttatccgcgt gtgtatcaac tgaagcgtga ctttccgcat ctgacgatgt
    21241 cgattaacgg tggtatcaag tcgctggaag aagctaaagc gcatttgcaa catatggatg
    21301 gcgtgatggt cgggcgcgag gcgtatcaga atccggggat tctggcggcg gtagaccgag
    21361 agatttttgg ttcctcggat accgatgccg atccggtggc ggtagtgcgc gccatgtatc
    21421 cgtacattga gcgtgaactc agccagggga cgtatctcgg ccatattacc cggcatatgc
    21481 tgggcttgtt ccagggtatt cctggcgcgc ggcagtggcg acgttattta agtgaaaatg
    21541 cccataaagc gggtgcagac attaatgtgc tggaacacgc gctcaaactg gtggcggata
    21601 agcgttaact tttcaccaaa aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg
    21661 cgttttgctg ttaaatcaat agattatttt tggcatgatt cttgtaatgc cagcaagaga
    21721 tttcatattt gggagagcat catgctggaa ctactttttg taattggctt ttttgtcatg
    21781 ctgatggtca ctggtgtttc gttgctgggt attatcgccg cgctgattgt ggcgacggcc
    21841 tttatgttcc tcggcggtat gctggcattg atgattaagt tgctgccgtg gctgctgttg
    21901 gcggttgcgg tggtgtgggt gatcagggcg attaaagcac caaaagtgcc gaaatatcag
    21961 cattatgacc gctggcgtta ctaagggatt gtgcggatga tcacaaccta aggttttatc
    22021 cttagaacaa aataggaatt gataatcaaa tctgtcacta ttgcgtgtca aacaaattca
    22081 tcgagctgta ccctacatac agccgaacta taaaagaaag ggcttcccag gtggaagccc
    22141 aatttctttt atggaatcaa caggctggaa ccctgcgtcg cccggctttc cagaatctca
    22201 tgcgcacgcc gcgcatcctt cagcggatat ttctgctgct cggcgacatc gaccttaatc
    22261 acaccgctgg caatcaaaga gaacagttca ttactggcct cggttaattc ctcccgcgtg
    22321 gtgatatagc cttgcaggga agggcgtgtc acatacaacg agcctttctg attgagaatg
    22381 cctaagttca caccggtaac cgcacctgat gagttgccaa aactcaccat taagccgcgg
    22441 cgttgcaggc aatccagcga ccgttcccag gtgtctctgc ccactgaatc gtacaccacg
    22501 cgcactttct tgccaccggt gatctctttt aaccgctcga ccagattctc ttcacgatag
    22561 ttaataacct gccatgcgcc cgcttttagc gcactctgcg ctttttgcgc ggttcccacg
    22621 gtgccgataa gtttcgcgcc cagggctttt gcccactggc aggcaatcaa gccaacgccg
    22681 ccagccgctg cgtggaacag gaactgttca tcgggtttaa tttcataggt tttgcgcagc
    22741 agataataaa ccgttaagcc tttcaggaag gatgccgcag cttgctcaaa agaaattgcc
    22801 gcaggcagaa tcgccgcttt atccgcatta atgttatgca tagagctgta agcgcctaat
    22861 gccgattgtg catagactac gcgatcgcct gccttaatat gctttacacc actgccgact
    22921 ttactcacga tgcctgccgc ttcggtgcct aatccgctgg gtagcgatgg tggcgggtaa
    22981 aggccgctgc ggatatacgt gtcgataaaa ttgatgccga tggctttatt tttgacctgg
    23041 atttcattct ccgccggatc ggcaggagtg aactccacgg cctgaagtac ttccgggcca
    23101 ccgtgcttgt gaaattcgat gcgcgttgcc atttaaactc ctttactgag cgacatatct
    23161 gttttaagcc taacagtagt cgccaggccg cgcggggatt ttttccgggc tgcggtcaca
    23221 acccggaata tctctgatca gtattgaaac ggcgtcgccg caatccagtt ttgccactgg
    23281 cgctcaaagt tggttagcgt taatacgccc cgttcacggc acgggcgagt ccaggtttgg
    23341 taatcattag cttcccgaca aacacaaatc atatattgcc tggcatcctg attttctggc
    23401 cgaaaacgcc aggccaggag tggcccacct ggtgtttgcg gtgcaatacc ggcttcgcca
    23461 tcatagatag ccctggcacc acgactccca ccaccgtttt cgacataaca ttgtaaagaa
    23521 cgcagtacag ccagcgccag ttgcgcactt tgccgccact ggaaagattg tgccaggctg
    23581 ttttcatcgc attgaatacc ttcccggcga atatcttcac aaagtgttgc cagggtttgc
    23641 gcggcagtgt tgatcccctg tgtatgacac aaaattgcgg catggtggct catggtattt
    23701 tgtatcgttt ctcgcacgtc gtcacgtagc agcccctgat tcaggtactg ttgcgcctca
    23761 ttaatcgtac tgagaatttg ttgcctctcg agaggccggt cctggctttt ctgatgagca
    23821 atatgtaatg cacagcggcg ggcgaaaact tgcccggcat tgagcgctgc tccacctggg
    23881 cgcgtgacac catgtgttcc ggcattttct ccggcagcat aacaaccagt tagcgatgta
    23941 cgtccccaaa tatcaacttc aattccgcca ttaagatgtt gattatttag cgtaaacttg
    24001 agcggtgagg ctgtgagatc atgaccgtgc atctgataca aacgaatagc cagaggattc
    24061 atctgtgtta atctcgccag cggttgtgcc aacagggcgt gattttgttg cagatagtcg
    24121 atgacatcat catccagttc ttgcaggttg aatgacgttc cgtccgctgc gggttctggt
    24181 tcccgtagaa aatcaagaaa aacatcgcgc cctttttgag tctcttcata aactgcgaca
    24241 tccagcaggc tggagccata ttccagcatt cgttccgcgt gaaacggcca ctggtaacct
    24301 ttacgaaata ttgcggaggc cagcatacga gtcgtggggt aataagctgc cagaaaatta
    24361 tactgacgcc cggagtggtc ctgcgaatag atgcgcggca tcgcctgcat gtaagtaccg
    24421 gaaagattcc acggaaactg gctgcgcggt gtaccgatac caaactgact ttccgtaaga
    24481 ttaaccagag ttatacctgc ttccagtgcc atgcccaacg cactaaaaca gttcaccgga
    24541 taaacactgt cacgataaag ttcacctggt ccgcccgtgg ctaaaaccag atgctggcag
    24601 cgaatcacga ccattcgcca gggattatcc cgacaggctt tatcgatggc gatcaccccc
    24661 acgactcgtc gattttcccc ttcaccactg gtcaggatat gaatcgccgt tgtgtgatcg
    24721 caaagaggaa tattaaggcg cattgcctcc tgggccagca ccttcaccat caggcgcgat
    24781 gttcgaggac cgcagctagt ggcacggcca aattcatcgt gatcggtttg ataacgcaac
    24841 gttgcgccaa agagatcttc aggcagatcc agacccagat atttcagtac ttcacaggtg
    24901 ttcaccgatc cgaccgcctc aacatatgcg gtgtcatgat ccatcgcacc acctgccgcc
    24961 agggtgttac ttaatgtcag aaaatggtca ccatttgcac gcgtattcgc ggtgtgaagc
    25021 gtttgtttat cggaaccaga acaggcggaa gtgccccaga aggctttgct ggaggcgacc
    25081 attacatcga cattttgccg tttcagctcg acggcagctc gccatcctgc cgcgccactg
    25141 cccagaacca gcgtgttgca gcagtactct ggtaaaatgg taccgttaag gtcgatacta
    25201 ccgctggctt gagccagcgg tgcgtaatgg ggcattttga tatcagtgag cgccgttagc
    25261 gtttcgtcaa tcattgcgtc ccacctattt catcagactt tcgattaagc cgctcattgc
    25321 catatagccc atgattagcg taaatacgct tatggcaata aacatgacgt tttcccagcg
    25381 tgagcaacga tatttgccca ttaacgaagg cttgttcgcc agccagaaca tacataaaac
    25441 agtcagcggc agaataatgg cgttaaaggc ctggctggca atcatcacca ataccgggcg
    25501 tgcgtggaac agcggcacaa ccagacaaag cagagacata accgtcatca ggatacggta
    25561 acctgggcta acttcgttgc tggtatcacg ccagtcagcc agcagccagg gcgttaacag
    25621 caaatttggg aactggctgg aaagcccggc tgcaacaata ccaataacaa agagccccac
    25681 ggcagctgag ccagcaatag gttcgagaat aatgaccatt tgcgaggcat tatttaaacc
    25741 tgtgccagtt tggtacaacg tacccgcagc cgcagccatc accgccgcac tgatgatgaa
    25801 catcattcct gcactgacaa tggcatcacg attttgatga ggcaaattgt ccacacccca
    25861 accattactt tttaccaggg tcgtgcgcat aagaaacatt gctggtgcta cggtggtacc
    25921 caccatgcca gaaattatca gaaatggcgt actgttatcg ccagcgacag gatcgggaac
    25981 agacggaata aatccggcca gcagatctgc gaccggcggc atcataatga aaaaattaat
    26041 cacaaacgcc agccccataa tgccaaccag cgcggagagg attttgtcga acccggcgtt
    26101 gctgttggtc cagagcatca cgttaatcat aatgaccatc gcgatagccc aggcgagagc
    26161 tgagataccg ccactaaacc aggtcgtgga ccatgttgcc attacatccg ccagaatacc
    26221 cattaccccc ataatggaac cgctcacggc aacggttaac ccgatcagaa acaacatcgc
    26281 aacgccggga tggatatggc gacgaaatgc agtcaacgcg cgttcaccag tgacggaagc
    26341 ataacgacca taaatacgga ttaaaaacca ggtaacaatg caagaaagcg ccacggccca
    26401 catgagcgag acaccatagt tagcacctgc tttagccata gtggtgatgc tgcctgttcc
    26461 gatgctgtag ccaataagaa agatgccagg tgcaaaggcc gcgagttttg tcattattcg
    26521 ccgaaataaa gaaggcgttt ggggaacaga ggttgtcata ttcattatct ttctttcctt
    26581 gtaagaggcg atcagcgtgc ctgaaagtgc ggttgacgct tctcagtaaa tgcctgactg
    26641 ccttcatgag catcttcagt cgtggcggaa aaagcgccag ccagtgcttc aagcgtcgag
    26701 gccacagcaa taccttctcc ggcatcaata acctgcttgg cgatctgcac tgcaacggga
    26761 gcacgctggg cgatacgttg tgccagttgc agtccggcgt cgtgtaacgc ctccctggcg
    26821 acaacgcgat tcaccagccc gcaggacagt gcccgctgtg catccagtgg ttcaccggta
    26881 aataccagtt ctttcaccag tgagcgccca agcagtgccg tcagccgctg cgttcctgac
    26941 cagccaggca ctgtcgccag cccggtttct ggtagtgcca tttgtgcttc ggcgctacaa
    27001 atacggatat cggcggtagc agccagttcc agaccaccgc cataagccag gccgtgcaac
    27061 agggcgatta cgggctgtgg cagttgtgcc agagcatcaa atgcctgatg accgcggcgg
    27121 atccagttgc gccacatatc gagtgcagaa agagaggtcc agcgatgaat atccgcgccg
    27181 acacaaaacg ctttttcact ggcactggtc agcagtacta cgcggatctc cgaatcagtt
    27241 tccaacgtgt ggcaatgcgc cagcagttgt tcgagcatct ctggggttaa tgcgttgagt
    27301 ttcgccgggc gattaatcac caaagtggca atgaaaccgt tgcgctggag gtcaatggtg
    27361 ccgttattca tggtgacttc cttgttgaaa atagagggga ttcattagtt tgagatcgtc
    27421 tgccagacgg agtggggcat cgctttgggc gagaatgtcg cgttcaggat caatacctgg
    27481 ggcaatttct gtgaccagta gcccgtctgg ggttaattcc agaacgcagc gttcagtaat
    27541 gtactgtact tgttgcccga gtcttattgc gcgtttgccg gagaacgtca cgtgggcgac
    27601 gtcctgtacc agttttttgg ctttgccttc tttaatgata cgcagctgac cctcttccag
    27661 ttgcagttgg gcaccggcat tgaaaaagcc agagaaaata atgcgtttcg catgtgaggt
    27721 gatatcaata aatccaccgc atccggcagt gacatgtgga cgagcgggga gatgtgagac
    27781 gttcactgag ccatctgcgc cgatttgtaa aaacgacatc agagtgagat caaaaccacc
    27841 accctggaaa taggtgaact gctggggaga gggcaaaaat gcttctgcat tagaggcaca
    27901 accgaactga aattccagca gtggaactcc gccaatcgcg ccctgttcca gcagccaggt
    27961 gacgtcaccg tggcgacctt gttccagtaa aatgcgtggt acgttggcag aaataccaaa
    28021 gccaatattt accgcatctc cactttgtag ttcttgcgcg actcgctggg caataactcg
    28081 cgccgggcca tgttccatat aacgaaaggc tgacagcgga cgactgattt cgccggaaat
    28141 ggcaggttca tattgcgttt gtgtcgtttg tgtttgttcc ggcgcaatca cgatgtaatc
    28201 caccagtaca cccggaatac gcacctcttt tggtttcaga cttccggcgg caacctgtcg
    28261 ttttacttgc gcaattatga tgccaccatt attgcgaacg gcagtagcct gttccagtgg
    28321 ccccagataa gccccttcat gttcataagt caggttgccg cgttcatcgg cagtggtagc
    28381 gcgaatgatc gccacctgcg gcacgatact ggggaaaaac agccagtcgt cgttagcaaa
    28441 tcgaacacgt tcaaccagag attgttgcgc cgctttttca ttcattgcac cgccctgcaa
    28501 caggggatcc acataggtat ccatgcctgt ttgtgtcagc acgccaggac gatgagccgc
    28561 cgcttcccgg tgcatatcaa ataagatccc ggaaggcaga ttccaggcgg ggatcgcgtt
    28621 gtcattgatc atttgccaga ttaacggagg cgtcattgat gatggaccgg aggggtagga
    28681 gccagcgatc acttttgcca gtaagcccgg ttgcgccaga tgatcgatgc cttttacgcc
    28741 atacatatca ccagcggcga tagggtggat ggaggtgatg ttttgcggat gtccggcacg
    28801 ttgaaagcgt tcacctattg ctgccagcac cgcatccgga caccccattc cactggagga
    28861 ggagacggtg acaactgcgc catcaacaat caaatctgca gcttccggtg cagtgatcgt
    28921 gcgtacagag ca
//
LOCUS       DACADA010000049        28320 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.050, whole genome shotgun sequence.
ACCESSION   DACADA010000049 DACADA010000000
VERSION     DACADA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.050"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..299)
                     /locus_tag="ISB17_002086"
     CDS             complement(<1..299)
                     /locus_tag="ISB17_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1980019.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(370..2484)
                     /gene="fusA"
                     /locus_tag="ISB17_002087"
     CDS             complement(370..2484)
                     /gene="fusA"
                     /locus_tag="ISB17_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP1980020.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="ISB17_002088"
     CDS             complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="ISB17_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP1980021.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="ISB17_002089"
     CDS             complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="ISB17_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP1980022.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="ISB17_002090"
     CDS             complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="ISB17_002090"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAP1980023.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="ISB17_002091"
     CDS             complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="ISB17_002091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAP1980024.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="ISB17_002092"
     CDS             complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="ISB17_002092"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAP1980025.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4687..5409)
                     /locus_tag="ISB17_002093"
     CDS             complement(4687..5409)
                     /locus_tag="ISB17_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1980026.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="ISB17_002094"
     CDS             complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="ISB17_002094"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1980027.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6609..6827
                     /locus_tag="ISB17_002095"
     CDS             6609..6827
                     /locus_tag="ISB17_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAP1980028.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="ISB17_002096"
     CDS             complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="ISB17_002096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP1980029.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7561..7761)
                     /locus_tag="ISB17_002097"
     CDS             complement(7561..7761)
                     /locus_tag="ISB17_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAP1980030.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="ISB17_002098"
     CDS             complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="ISB17_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAP1980031.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="ISB17_002099"
     CDS             complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="ISB17_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAP1980032.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10258..12171
                     /locus_tag="ISB17_002100"
     CDS             10258..12171
                     /locus_tag="ISB17_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1980033.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12171..13193
                     /locus_tag="ISB17_002101"
     CDS             12171..13193
                     /locus_tag="ISB17_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1980034.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13187..13405
                     /locus_tag="ISB17_002102"
     CDS             13187..13405
                     /locus_tag="ISB17_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAP1980035.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13459..14328
                     /locus_tag="ISB17_002103"
     CDS             13459..14328
                     /locus_tag="ISB17_002103"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAP1980036.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14383..14787)
                     /locus_tag="ISB17_002104"
     CDS             complement(14383..14787)
                     /locus_tag="ISB17_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP1980037.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15089..15721
                     /gene="crp"
                     /locus_tag="ISB17_002105"
     CDS             15089..15721
                     /gene="crp"
                     /locus_tag="ISB17_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAP1980038.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15772..17862
                     /locus_tag="ISB17_002106"
     CDS             15772..17862
                     /locus_tag="ISB17_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980039.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="ISB17_002107"
     CDS             complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="ISB17_002107"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAP1980040.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="ISB17_002108"
     CDS             complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="ISB17_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAP1980041.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19830..20432)
                     /locus_tag="ISB17_002109"
     CDS             complement(19830..20432)
                     /locus_tag="ISB17_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAP1980042.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(20422..20589)
                     /locus_tag="ISB17_002110"
     CDS             complement(20422..20589)
                     /locus_tag="ISB17_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAP1980043.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="ISB17_002111"
                     /gene_synonym="rotA"
     CDS             complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="ISB17_002111"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAP1980044.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21537..22718
                     /gene="tsgA"
                     /locus_tag="ISB17_002112"
     CDS             21537..22718
                     /gene="tsgA"
                     /locus_tag="ISB17_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAP1980045.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22980..25523
                     /locus_tag="ISB17_002113"
     CDS             22980..25523
                     /locus_tag="ISB17_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAP1980046.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVARIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25520..25846
                     /gene="nirD"
                     /locus_tag="ISB17_002114"
     CDS             25520..25846
                     /gene="nirD"
                     /locus_tag="ISB17_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAP1980047.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25973..26779
                     /gene="nirC"
                     /locus_tag="ISB17_002115"
     CDS             25973..26779
                     /gene="nirC"
                     /locus_tag="ISB17_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAP1980048.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26798..28171
                     /gene="cobA"
                     /locus_tag="ISB17_002116"
     CDS             26798..28171
                     /gene="cobA"
                     /locus_tag="ISB17_002116"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP1980049.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6853 a         7579 c         7151 g         6737 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg
      181 taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg
      241 tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg
      301 gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa
      361 cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc
      421 atcatacttc aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg
      481 agttgcatat ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac
      541 ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc
      601 ggtgttctct tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt
      661 cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga
      721 ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta
      781 gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta
      841 ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga
      901 acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga
      961 ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc
     1021 aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat
     1081 gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt
     1141 ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc
     1201 ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat
     1261 acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt
     1321 cagaccgatt gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt
     1381 agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac
     1441 ggtatcacca gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa
     1501 cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg
     1561 ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg
     1621 ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc
     1681 agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt
     1741 gatttctgct tcagtcagtt cttcaccacc cagatatttt tccatcagct cttcagaagc
     1801 ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc
     1861 cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt
     1921 cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag
     1981 cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc
     2041 acccatgcgg tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg
     2101 acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc
     2161 accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt
     2221 gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt
     2281 agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt
     2341 tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg
     2401 ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg
     2461 tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa
     2521 gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga
     2581 ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg
     2641 tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag
     2701 agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc
     2761 attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg
     2821 agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc
     2881 agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt
     2941 agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa
     3001 cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     3061 ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     3121 cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     3181 acgagcctgc ttacggtctt taacaccgga gcagtcaagc gcaccacgta cggtgtggta
     3241 acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     3301 caagttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     3361 acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     3421 acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     3481 gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt
     3541 agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt
     3601 tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc
     3661 tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg
     3721 ttcgaaattc gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta
     3781 atgggggcat tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca
     3841 tcttgcaata atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc
     3901 gtcagccagg gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca
     3961 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca
     4021 aagggtgtct gcggatctaa cccacgttcg cgcagtgaag ccgcgcaaat ccagcactgt
     4081 tcaatatcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4141 tcgggctttt gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4201 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccggcc tgcagttgta
     4261 ccatgaggcg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4321 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4381 ggtttgacga agcaagcccc agtcttccgg cctccgtttc atcaacaacg ccacggcgta
     4441 atgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4501 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4561 cttcccgata gaaaaaaacg ctgcttaact catggccttc tgctatcagc gcctgcgcaa
     4621 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4681 aacgcattac ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata
     4741 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4801 gaagatccct ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac
     4861 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4921 aacagaagag gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt
     4981 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5041 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5101 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5161 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5221 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5281 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5341 ctggtcaaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5401 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5461 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5521 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5581 ttttagcaga gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5641 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
     5701 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5761 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
     5821 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5881 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     5941 aaaccagacc agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6001 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6061 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct
     6121 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6181 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6241 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6301 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6361 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6421 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6481 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6541 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6601 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6661 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6721 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6781 cgaacatcgc gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag
     6841 cgggaattcc ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg
     6901 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca
     6961 ccgcagcaac cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca
     7021 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7081 ttctgaccag ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7141 atttcaaccg gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt
     7201 tcatcaacgc ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga
     7261 ccgtaagcgt cattcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7321 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7381 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7441 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7501 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7561 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc
     7621 tttgtctgct tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt
     7681 ttcgcgccac atcgccattg aatcctgagc ctgacaggcc gggcattttg cgcccgcaat
     7741 aaaacgtttt cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg
     7801 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7861 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7921 atccaggcgg cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt
     7981 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8041 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg
     8101 caaatgcgga aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt
     8161 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg
     8221 cgtggcgtcg ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat
     8281 atcccgctcc agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8341 accaaaacgc ccaaagccca cgacaataac ctgcggttta tcatcgttga cccatggttt
     8401 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcta ccagcttcat
     8461 cagcagcggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8521 gccctggaat aagcgttgtg aagaagcgga agaaaagagg acaaaggcaa actcaccgcc
     8581 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8641 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca
     8701 caacagatgg gtataaagca ccccgaggtt gagtgacatg ccgacagaga taaagaacaa
     8761 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8821 ttccgccagt agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc
     8881 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     8941 accagaagct gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat
     9001 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9061 acccgccaac aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag
     9121 taccgagaaa cctaactggc cggattcgct gcgattcatc cctttttcac gcatcaactg
     9181 caatgccatt gcggttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9241 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9301 cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag
     9361 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9421 gtcgctaata aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa
     9481 cactgcacca atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa
     9541 cagcactcct gcgagtaaaa aatcagaacc ttccatcagc ggcctcctgg agacaacgga
     9601 tttgccagcc agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt
     9661 ctcgcccagt aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt
     9721 tcaaaggggc gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca
     9781 ctttccggct ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc
     9841 cccggtccgc tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc
     9901 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
     9961 gcctgctcac gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg
    10021 gtaacattgc tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga
    10081 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10141 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10201 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10261 attgtttttt cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10321 gccaccatca accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10381 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10441 ggaagctggc aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg
    10501 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc
    10561 aacgaacgta acgacgggca agccattgca accattcatg gcaagctgga tgctattgac
    10621 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10681 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10741 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10801 ctcgatgccg ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg
    10861 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa
    10921 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    10981 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtagcgca tctgcaaagt
    11041 tatatcgacc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgtattaag
    11101 atgctggagc gtatggagct gattgccccg gcgcacgtcg acaacccgtt ccgctttagc
    11161 ttccgcgcgc cggaaagcct gcctaatccg ttattaaaga tggaaaaagt cagcgcaggc
    11221 tatggtgatc gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt
    11281 ggtctgctag gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11341 cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc
    11401 cagcatcaac ttgaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta
    11461 gcgccgcagg agctggaaca aaaactgcgt gattacctcg gcggctttgg tttccagggc
    11521 gataaagtaa ccgaagaaac gcgccgcttt tccggtgggg aaaaagcccg cctggtgctg
    11581 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11641 gacctcgaca tgcgtcaggc actcactgaa gcattaatcg agttcgaagg cgcgctggtt
    11701 gtcgtctcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11761 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11821 gtacaaaagc aggaaaacca ggccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11881 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt
    11941 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12001 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12061 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12121 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12181 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12241 ttagcaattg tcatctgcaa accatgctgc cgcgtctgtt tcgtcgcaag gtgaaattca
    12301 ccccgcactg gcatcgactg gagttgcccg acggcgattt tgtcgatctc gcatggagtg
    12361 aagatcctgc acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca
    12421 gtctcaatag cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12481 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12541 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc
    12601 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12661 caaaagaagg taatgatctc ccgattgatg cggcggtgat tgtctctgcg ccgtttatgc
    12721 tggaggcctg tagttaccat atggaaaagg gcttttcccg catttatcag cgttacttgc
    12781 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga
    12841 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg
    12901 ccagaattca cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga
    12961 tgctcaatcg gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg
    13021 atcatcaggt gatcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg
    13081 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13141 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13201 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13261 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13321 gctacagtgc ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat
    13381 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag
    13441 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13501 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13561 catgcagccg aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg
    13621 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13681 gacttcggcc ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct
    13741 cgcaaatatc tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc
    13801 accccctggc aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc
    13861 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg
    13921 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    13981 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14041 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca
    14101 aacccgttcg cggcaaaagg tatcccgtca ctggatgaaa gctttgtggt gatccatttt
    14161 cgtaatctgg aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt
    14221 tcccacatca atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt
    14281 atgctgccgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac
    14341 gccggataaa atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc
    14401 atacgagtga gtaatattca ccgctttttc cagcatcagc gccactgagc aatatttctc
    14461 ggcagaaaga tcaaccgcac gcgcaaccgc tgcgtctttc agatcgcgac cggtgacgat
    14521 aaaatgtaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa
    14581 ttttacttca caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat
    14641 ggcactgcaa ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc
    14701 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14761 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14821 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14881 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt
    14941 gatgtactgc atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt
    15001 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15061 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15121 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15181 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15241 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15301 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15361 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15421 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15481 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct
    15541 ggcaaaacaa ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15601 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15661 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15721 atcccgtcag agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15781 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15841 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt
    15901 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta
    15961 atcattggtg cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa
    16021 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16081 ctggggccat tgcacgcaaa attgcttccc gcatcgctgc tcgccgccat ttttaccctc
    16141 agtctggcgg gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg
    16201 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag
    16261 tcactaagtc tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt
    16321 acccagcaca ccgaccctga aaaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg
    16381 gtcgatctaa ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc
    16441 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16501 gtcagtttgc atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa
    16561 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16621 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16681 atcgcccgcc aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16741 attgctcgtg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16801 cagcggcgaa tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg
    16861 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg
    16921 ctgcatctgc cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc
    16981 tatggcgcaa cccgtctgag gattgtaaat cgctccgtag gaaccgtggt cgggttaatc
    17041 attgcaggcg tggcgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg
    17101 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt
    17161 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17221 cttccgcgtc ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg
    17281 ctgtggccgc agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc
    17341 tatcaggacg cgattcgctt gattctgagc gaggacccgc aacctactcc actggcctgg
    17401 cagcgaatgc gggtaaatca ggcgcataat actctgtata actcattgaa tcaggcgatg
    17461 caggaaccgg cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17521 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17581 ccaccggaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17641 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17701 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt
    17761 ggccatctga acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac
    17821 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17881 atgaggtgtt cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acgccccaac
    17941 caccttcgcc accgcgtggg cgaaacgatg catcccttca tcgatatccg catcttccac
    18001 caccagcgac ggcgcaaaac gcatcacatc aggcccggca ttcagcacca ttacgccttc
    18061 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tactgtggtt tcagctctgc
    18121 gccaatcaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18181 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaata ccttccagca cttcaggggt
    18241 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18301 ggaaccgtga gaaccaggat gaaacgcgga agcaatttcc gccgtggtca gcatggcgct
    18361 aatcgggaag ccgccgccta acgctttcgc agaggtcaga atatccggcg tcacgccgta
    18421 gtgcatgtaa gcaaacaaat cgccggtccg ccccatcccg cactgtactt catcaaacac
    18481 caataatgcc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18541 tgccgtcaca ccgccctcac cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18601 catcaccgct ttcactgcgt ggagatcgtt aaagggaacg tggatgatgt ctgccggttt
    18661 tggcccaaag ccatcggaat attttggttg cccgcccacc gagacggtaa acagcgagcg
    18721 accatgaaaa gcgttatgga aggcgataat tttggttttg aacgggctat gacgcacaca
    18781 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18841 caccacgcgt tcggcaaacg ttgcctcaat cagtttacga ccaagacgca gcgccggttc
    18901 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    18961 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19021 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19081 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19141 aattgccatc tcatgatcac cctgttacgc ataaaccaat gtgaaattat aatcacaaaa
    19201 tatgcataaa aaatcactta atagcaatca gaaatcagcg atgcaggaaa ttagccagca
    19261 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ttgcacacct tccagatccc
    19321 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acttcaaagc
    19381 actccggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtgagtggat
    19441 ttgccagccc cttaaatacg cccacaccat tatgtgtaat cggcgaggtt ttgccgtgca
    19501 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg tgccattgcc tggtgaccga
    19561 ggcagacgcc aagaatcggc aaacgcccgg catagtgacg aataacgtca agggagatcc
    19621 cggcttcatc tggcgtacag gggccgggtg agatgacaat tttttgtggt ttaagggcgt
    19681 cgatatccgc caacgtcaac gcatcgttgc gcttaaccaa cacatccgcc cccagttcac
    19741 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atcaataagc aggatcatgg
    19801 cggctccggt acgaaagaac cgcgctattt tactcagatt ccccgacttc gcttaccact
    19861 ttgctaaata tcatctgcag cgcggtgaga tcccccattg ccccattctg atttgcctga
    19921 ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    19981 tgctcgaaga agatccgctg tgccaaacca cttcccaccc ggaagggatg cagcacgttg
    20041 atttcacaat agtaatgcgc caaccgctcg acgaatttat ctttctcaag gctagtcaga
    20101 tacttctctt cctccaaatc ctgcatcagg gcattgccct ctttttcgag ataggcaaag
    20161 tggcagaacg gcgtatctcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20221 aaaatatcct gatacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20281 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20341 cgctgctgct ggcggatgtt gagcttgtta cgcatgatat cgagacccgg gtagagatat
    20401 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagcgctt caaggcgcgc
    20461 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20521 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20581 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20641 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20701 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaacg
    20761 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20821 aataccgcgt aaccgaaatc acgctgaccg tggtcaagga aggcgttatc ggcaacgttg
    20881 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    20941 cgcgtgttgc gcaggccgtt atcagcttca tttttgattg gcgggtttgg ttttttctgc
    21001 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21061 gtcgtgttgt tataaaaacc actgttcaca taatcgacaa agttttgcac agacactggc
    21121 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21181 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21241 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21301 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21361 atctgaaata tatgtaacct gaggataact ttttattccg tgatcaaaat cacctcttaa
    21421 aatgcaattt agcaaccgat tgcaataaaa catttaatca gatcacaaaa tcgcctaaaa
    21481 tcgcccgctc gcggcatagc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21541 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ttcctacgca ctgaccggtg
    21601 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21661 tttccagcat gagtaacacc ttcaccttcc tcaatgccgg cattttaatc tctatcttcc
    21721 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21781 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcggcgg
    21841 cgatgttcat tctcggggtg gtcagcggca tcaccatgtc gattggtaca ttcctggtaa
    21901 cacaaatgta tgaaggacgt cagcgcggtt cacgcctgtt atttaccgac tccttcttca
    21961 gtatggccgg gatgattttc ccaatgatcg ccgcatttct gctggcgcgt agcattgagt
    22021 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    22081 gctgtgagtt cccggcgctg ggcaaacatg cgcccaaaac ggatgctccg gtagagaaag
    22141 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22201 agttaggttt tatctcctgg gtgccggaat atgccaaagg cctgggcatg agcctgaacg
    22261 acgcaggcac gctggtaagt aacttctgga tgtcgtacat ggtcggcatg tgggcattca
    22321 gctttattct tcgcttcttt gatttgcaac gtatcctgac cgtactggct ggtctggctg
    22381 cgattctgat gtacgtcttt aacaccggaa caccggcaca tatggcgtgg tcaattctcg
    22441 ccctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22501 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcgggacc atcggtacta
    22561 tgttgacctt tgtggttacc ggcccgattg ttgaacatag tggtccgcag gcggcgctgc
    22621 tgacggcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22681 gccgtcaccg tcagcataac accctgacct ctcattaatt gttcatgccg ggcggcacta
    22741 tcgtcgtccg gccttttcct ctcttaccct gctacgtgca tctatttcta taaacccgtt
    22801 caatttgtct gttttttgca caaacatgaa atatcagaca attccgtgac ttaagaaaat
    22861 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22921 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    22981 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23041 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23101 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23161 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23221 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23281 tttatgacaa gctgatcatg gcgaccggtt cctacccgtg gatcccgcca atcaaaggtt
    23341 ctgatactca ggactgcttt gtctatcgca ctattgaaga cctcaacgcc attgaatcct
    23401 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23461 ctggcgcgct gaaaaactta ggcattgaaa cccacgttat cgaatttgcc cctatgctga
    23521 tggcggaaca gcttgatcag atgggcggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23581 gcgtgcgcgt tcacaccagc aaaaacactc ttgagattgt gcaggaaggt gttgaagcgc
    23641 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23701 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23761 ggggtatcgt cataaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23821 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23881 aggtcgccgt tgaccatatt cttggtagcg aaaacgcctt tgaaggtgca gaccttagcg
    23941 ccaagctgaa actgctgggc gtagacgtag gcggtattgg tgatgcgcac ggtcgcacgc
    24001 ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga gatctacaaa cgcctgattg
    24061 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24121 gcaacctgct gcaactggtg ctgaacgcta tcgaactgcc ggaaaacccg gattctctga
    24181 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24241 gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga cctgattgct gctatcaaca
    24301 aaggctgcca caccgttgcg gcgctgaaag ccgaaaccaa agccggtacg ggctgcggcg
    24361 gttgtattcc gctggtcact caggtactga acgcggaact ggcgaaacag ggtatcgaag
    24421 ttaacaacaa cctgtgtgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24481 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggttacggtt
    24541 gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24601 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaaa
    24661 aagacggcac ctactcggtg atcccgcgtt ctccgggtgg tgaaatcacc ccggaagggc
    24721 tgatggcagt ggctcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    24781 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggagatctgg cgtcagctga
    24841 ttgaagcagg tttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24901 gcgtgggtag cacctggtgc cgctatggcg ttggcgacag cgtcggcctc ggcgtggaac
    24961 tggaaaatcg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25021 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25081 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25141 cagcggatat cgatcgcgaa acgctgatta aatatcttga ccgcttcatg atgttctaca
    25201 tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggtggca
    25261 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgca catctggaag
    25321 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaataccc
    25381 cgtctgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25441 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25501 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25561 gcctgaaacc ggcgtctgcg cgctgttagg tgacgaacaa gtagcaattt tccgcccgta
    25621 tcacagcgac caggtgtttg cgatcagcaa catcgatccg ttcttcgagt ccagcgtgct
    25681 gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25741 gcagcgtttt cgcttaagcg acggcttgtg catggaagac gaacagtttt ccgtcaagca
    25801 ttacgacgcg cgagtgaaag acggcgtggt gcagctgcgc ggttaatatt ttaacgggag
    25861 gcgcaatgcc tccccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    25921 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    25981 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26041 cccgctcggc ttttgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26101 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggtgc
    26161 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26221 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26281 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtctttgttg ccatgctcta
    26341 tagctggggt ggcggtagcc tgctgccggt cgataccagc atcgttcact ccgtcgcgct
    26401 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26461 ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26521 ctggtggtgt ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26581 gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26641 tcataacctg ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt
    26701 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    26761 tgaaacggct gccggttaat tattaagggg tttttacgtg gatcatttgc ctatattttg
    26821 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    26881 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    26941 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27001 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    27061 cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27121 cgccagcttt attatgccgt cgattattga ccgctcgccg ctcatggtag cggtctcctc
    27181 tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc
    27241 gttacatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27301 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta atgaccgcct
    27361 ggcgcagtcg ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    27421 caatgaaccg ctcgatcatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    27481 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    27541 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    27601 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27661 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    27721 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc
    27781 gggtattacc gcagcctctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    27841 ttatgcccag agcgtacgct taattactgg acacttaaaa accggtggcg agctggactg
    27901 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    27961 aactattcag caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tagcaattgt
    28021 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28081 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28141 cgataaattg aactggttct ctaaccatta atttaaccag ggccagagaa gtctggcctt
    28201 tttcgtttac aactctcttt atatattcag cagataaaaa tataacccct taataaatat
    28261 tgtctgctaa tgggttatat tcaccgttac agcaacttac aaaattaagc caatcgctca
//
LOCUS       DACADA010000050        28240 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.051, whole genome shotgun sequence.
ACCESSION   DACADA010000050 DACADA010000000
VERSION     DACADA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.051"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            338..1477
                     /locus_tag="ISB17_002117"
     CDS             338..1477
                     /locus_tag="ISB17_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAP1980050.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTLGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1483..1926
                     /gene="wzb"
                     /locus_tag="ISB17_002118"
     CDS             1483..1926
                     /gene="wzb"
                     /locus_tag="ISB17_002118"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAP1980051.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYALLERSAHQWAQALNAEQV"
     gene            1929..4091
                     /gene="wzc"
                     /locus_tag="ISB17_002119"
     CDS             1929..4091
                     /gene="wzc"
                     /locus_tag="ISB17_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAP1980052.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLTGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKVLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4220..5059
                     /gene="wcaA"
                     /locus_tag="ISB17_002120"
     CDS             4220..5059
                     /gene="wcaA"
                     /locus_tag="ISB17_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAP1980053.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5062..5550
                     /gene="wcaB"
                     /locus_tag="ISB17_002121"
     CDS             5062..5550
                     /gene="wcaB"
                     /locus_tag="ISB17_002121"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAP1980054.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5547..6764
                     /gene="wcaC"
                     /locus_tag="ISB17_002122"
     CDS             5547..6764
                     /gene="wcaC"
                     /locus_tag="ISB17_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAP1980055.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6739..7959
                     /gene="wcaD"
                     /locus_tag="ISB17_002123"
     CDS             6739..7959
                     /gene="wcaD"
                     /locus_tag="ISB17_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAP1980056.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNYRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            8040..8786
                     /gene="wcaE"
                     /locus_tag="ISB17_002124"
     CDS             8040..8786
                     /gene="wcaE"
                     /locus_tag="ISB17_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAP1980057.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLHFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8802..9350
                     /gene="wcaF"
                     /locus_tag="ISB17_002125"
     CDS             8802..9350
                     /gene="wcaF"
                     /locus_tag="ISB17_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAP1980058.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            9377..10498
                     /gene="gmd"
                     /locus_tag="ISB17_002126"
     CDS             9377..10498
                     /gene="gmd"
                     /locus_tag="ISB17_002126"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP1980059.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10501..11466
                     /gene="fcl"
                     /locus_tag="ISB17_002127"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10501..11466
                     /gene="fcl"
                     /locus_tag="ISB17_002127"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP1980060.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11469..11948
                     /locus_tag="ISB17_002128"
     CDS             11469..11948
                     /locus_tag="ISB17_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAP1980061.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGSDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11945..13168
                     /gene="wcaI"
                     /locus_tag="ISB17_002129"
     CDS             11945..13168
                     /gene="wcaI"
                     /locus_tag="ISB17_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAP1980062.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAAMVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13171..14607
                     /gene="cpsB"
                     /locus_tag="ISB17_002130"
     CDS             13171..14607
                     /gene="cpsB"
                     /locus_tag="ISB17_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAP1980063.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14712..16082
                     /gene="cpsG"
                     /locus_tag="ISB17_002131"
     CDS             14712..16082
                     /gene="cpsG"
                     /locus_tag="ISB17_002131"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP1980064.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16138..17532
                     /gene="wcaJ"
                     /locus_tag="ISB17_002132"
     CDS             16138..17532
                     /gene="wcaJ"
                     /locus_tag="ISB17_002132"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAP1980065.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17534..19012
                     /gene="wzxC"
                     /locus_tag="ISB17_002133"
     CDS             17534..19012
                     /gene="wzxC"
                     /locus_tag="ISB17_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAP1980066.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19481..20761
                     /gene="wcaK"
                     /locus_tag="ISB17_002134"
     CDS             19481..20761
                     /gene="wcaK"
                     /locus_tag="ISB17_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAP1980067.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDKQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20758..21978
                     /gene="wcaL"
                     /locus_tag="ISB17_002135"
     CDS             20758..21978
                     /gene="wcaL"
                     /locus_tag="ISB17_002135"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAP1980068.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATSLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21989..23383
                     /gene="wcaM"
                     /locus_tag="ISB17_002136"
     CDS             21989..23383
                     /gene="wcaM"
                     /locus_tag="ISB17_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAP1980069.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23558..24451
                     /gene="galF"
                     /locus_tag="ISB17_002137"
     CDS             23558..24451
                     /gene="galF"
                     /locus_tag="ISB17_002137"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAP1980070.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDNASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24824..25909
                     /gene="rfbB"
                     /locus_tag="ISB17_002138"
     CDS             24824..25909
                     /gene="rfbB"
                     /locus_tag="ISB17_002138"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP1980071.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYFFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNSLEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            25909..26808
                     /gene="rfbD"
                     /locus_tag="ISB17_002139"
     CDS             25909..26808
                     /gene="rfbD"
                     /locus_tag="ISB17_002139"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP1980072.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26866..27744
                     /gene="rfbA"
                     /locus_tag="ISB17_002140"
     CDS             26866..27744
                     /gene="rfbA"
                     /locus_tag="ISB17_002140"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP1980073.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27749..>28240
                     /gene="rfbC"
                     /locus_tag="ISB17_002141"
     CDS             27749..>28240
                     /gene="rfbC"
                     /locus_tag="ISB17_002141"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP1980074.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQRIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHEKNIIWNDLLLNIKWPST
                     ALIT"
BASE COUNT         7032 a         6811 c         7637 g         6760 t
ORIGIN      
        1 tattggagag ccaatattca attaccataa tgtcacctta tattcggaag ttaatttccg
       61 caataaccct ttctcatcaa cgactgtatg cagagagggt gtgaaaaatt aagctaaatc
      121 agtgtgttgg tagctaaaaa gccaggggcg gtagcgtgtc tggatgcctg aaagaccagt
      181 ctgaattatt ctgtcaatag cctgcggatg aaatcaattt tttttaggac tgatgccagt
      241 taaattttta ttcaattaac tgcaaggtaa atgtgcatgg cgacattgct attaatagtg
      301 cacaggataa ttactctgcc aaagtgataa ataaacaatg atgaaatcca aaatgaaatt
      361 gatgccatta ttggtgtcag taaccttgat aagcggttgc acagtacttc cgggcagcaa
      421 tatgtcgacg ctggggaaag acgtcatcaa acagcaggac gctgatttcg atctcgacaa
      481 aatggtgaat gtttatccgc tgaccccgcg cctgattgat caattacgcc cacgcccgaa
      541 tgtagcgcgc cccaatatga cgctggaaag cgagatcgcg aattaccagt atcgcgtcgg
      601 gccgggggac gttcttaatg tcaccgtctg ggatcacccg gaactcacca cgccagccgg
      661 tcagtaccgc agctccagcg acaccggcaa ctgggtacag cctgacggca ccatgttcta
      721 cccgtatatc ggcaaagttc acgtggtggg gaaaacgctc gctgaaatcc gcagtgatat
      781 taccgggcgc ttagcgacgt acatcgctga cccgcaggtg gacgttaata tcgccgcctt
      841 ccgctcgcaa aaagcctata tctccggtca ggtgaataaa tccggtcaac aggccattac
      901 caacgtgcca ctgaccattc tcgacgccat caacgccgca ggtggcctga ccgacaccgc
      961 tgactggcgc aacgtggtgc taacacacaa tggtcgtgaa gagcgcattt ctttgcaggc
     1021 gctgatgcaa aacggcgacc tcaatcagaa ccgactgctt taccccggcg atattcttta
     1081 cgtgccacgt aatgatgatc tgaaagtgtt tgtgatgggt gaagtgaaga aacagagcac
     1141 cctgaaaatg gactttagcg gcatgaccct gactgaagcc ctgggcaatg ctgaaggcat
     1201 cgacatgacc acctccaacg ccagcggcat ctttgtcatt cgtccgctga aaggcgagag
     1261 cgggcgtaac ggcaagattg ccaatatcta ccagctggat atgtccgatg cgacgtcgct
     1321 ggtaatggcg acagaattcc gcctgcaacc ttatgacgtg gtgtatgtca ccaccgcccc
     1381 ggtatcccgc tggaaccgtc tgatcaatca gttgctgcca actattagcg gtgtccgtta
     1441 catgacggat acagccagcg acattcataa ctggtaatcg tcatgtttaa caacatctta
     1501 gttgtctgtg tcggcaatat ttgccgttcc ccgacggcgg aacgcttact gcaacgttat
     1561 cacccggagc taaaagtgga gtccgctgga ctcggcgcgt tggtcggtaa gggcgctgat
     1621 cctaccgcta tcagcgtcgc cgcagaacat caactgtccc tggaaggtca ctgtgcacgg
     1681 caaatcagcc gccgcctgtg tcgcaactac gacctgattc tgaccatgga aaagcgccat
     1741 atcgaacgct tatgcgagat ggcacccgag atgcgcggca aagtgatgct gtttggtcac
     1801 tgggataacg aatgtgaaat ccccgatcca tatcgcaaaa gccgggaaac gtttgcagcg
     1861 gtgtacgcat tacttgaacg gtctgcccac cagtgggcgc aggcattgaa cgcagagcag
     1921 gtataagaat gacagaaaaa gtaaaacaac atgccgctcc ggtaacgggc agtgatgaaa
     1981 tcgatattgg tcgcctggtt ggcaccatca ttgaagcacg ctggtgggtg attggcatta
     2041 ccgccgtatt cgccctctgt gccgtggttt acaccttctt cgctacgccg atatacagcg
     2101 ccgacgcact ggtacaaatc gaacaaagca gcggcaattc gttagtgcag gatatcggat
     2161 cggcattagc caacaaaccg cctgcatcgg acgccgagat ccagttgatt cgttcgcgtc
     2221 tggtgcttgg taaaacggtg gacgatctcg accttgatat tgcggtgagc aaaaacacgt
     2281 tcccgatttt cggtgcgggc tgggagcgcc tgacgggacg ccagaacgag acggtgaaag
     2341 tgaccacctt taaccgaccg aaggagatgg cggatcaggt gtttacgctt aatgtgctgg
     2401 acaacaaaaa ctacaccctg agcagcgatg gcggctttag cgcccgtggg caagcgggcc
     2461 agatgctgaa aaaagaaggc gtcacgctga tggttgaagc cattcacgcc agcccgggca
     2521 gtgagtttac cgtcaccaaa tactccacgc tggggatgat caaccaactg caaaacagcc
     2581 tgacggtaac ggagaacggc aaagacgcag gtgttctgag cctgacttac accggtgaag
     2641 atcgcgaaca gatccgcgac attcttaaca gcatcgcccg taactatcag gaacaaaata
     2701 ttgagcgcaa atcggcggaa gcgtcgaaaa gcctcgcttt cctcgcgcaa cagttaccgg
     2761 aagtacgtag ccgccttgat gttgccgaaa acaaactgaa tgccttccgt caggataaag
     2821 attcagttga tctgccgctg gaagcgaaag cggtgctcga ttcgatggtg aacatcgacg
     2881 cgcagttgaa cgaactgacc tttaaagagg cggaaatctc caagctgtac accaaagttc
     2941 acccggcgta ccgcacgctg ctggagaaac ggcaggcgct ggaagacgaa aaagccaaac
     3001 ttaatggtcg cgtaacggcg atgccgaaaa cccagcagga aattgtgcgt ctgacccgcg
     3061 atgtcgagtc tggtcagcag gtctatatgc aactgctgaa taaagagcag gagctgaaaa
     3121 tcaccgaagc cagcaccgtc ggcgatgtgc gcattgtcga cccggcaatc acccagccag
     3181 gtgtactgaa accgaagaaa gtgctgatta tccttggggc gattatcctt ggcctgatgc
     3241 tctctatcgt gggtgtgctg ctgcgctcgt tgtttaatcg cggtatcgaa agcccgcagg
     3301 tgctggaaga acacggtatc agcgtttatg ccagcatccc gctgtcggag tggcagaaag
     3361 cgcgcgatag cgtcaaaacc atcaaaggga ttaaacgcta taaacagagc cagctactgg
     3421 cggtggggaa tccaaccgat ctggcgattg aagccatccg tagtctgcgt accagtctgc
     3481 acttcgcgat gatgcaggca cagaacaatg tgttgatgat gaccggggtt agcccgtcaa
     3541 tcggtaaaac ctttgtctgc gccaacctgg cggcggtgat tagccagacc aataaacggg
     3601 tgttgttgat cgactgcgat atgcgcaaag gctacaccca cgagctgttg ggcactaata
     3661 acgttaatgg cctgtcggaa attctgattg gacagggcga tattactacc gctgctaaac
     3721 cgacctctat tgccaaattt gacctgatcc cgcgcggtca ggtaccgcca aatccttctg
     3781 aactgttaat gagcgaacgc tttgccgaac tggtgaagtg ggcgagtaaa aactacgacc
     3841 tggtgttgat tgatacgccg ccgattctgg cagtgaccga tgcggcaatt gttggtcgtc
     3901 atgtcggaac cacgttgatg gtggcgcgtt atgcggtcaa cacattgaaa gaagtggaaa
     3961 ccagtctgag ccgctttgag caaaacggta ttccggtgaa aggggtgatt ctgaactcca
     4021 tcttccgccg cgccagcgcg tatcaggatt atggctatta cgaatacgaa tataagtcgg
     4081 atgcgaaata acgagatccg ctgtcggatg cgatgctgac gcatcttatc cgacctacgt
     4141 tcacgccgca tccggcaaca gaacctgtaa accagcttat aaaccggcag aacatctcga
     4201 tactccggga gaacaaccta tgaaagacaa tccgctgatt tcaatctata tgccgacctg
     4261 gaaccgccaa caactggcga ttcgggcgat aaaatcggtc ctgcgccagg actacaacaa
     4321 ctgggagatg atcatcgtgg atgattgctc cacctcctgg gaacaactac aacagtacgt
     4381 caccgccctc aacgatccgc gtatcactta cattcacaac gacattaaca gcggggcgtg
     4441 cgcggtacgt aaccaggcga ttatgctggc gcaaggggaa tacatcaccg ggatcgatga
     4501 cgatgatgaa tggacaccca accgcctgag cgtcttcctc gcccataaac agcaactggt
     4561 cacacacgcc tttttgtacg ctaacgacta cgtttgccag ggcgaagttt attcccaacc
     4621 ggcaagcttg ccgctgtatc ccaaatcgcc gtattcacgc cgcctgtttt acaaacgcaa
     4681 tatcattggc aatcaggtct ttacctgggc gtggcgtttc aaagagtgct tgttcgatac
     4741 cgaactgaaa gcggcacagg attacgacat cttcctgcgg atggtggtgg agtacggcga
     4801 accgtggaaa gtggaagagg cgacgcagat cctgcatatc aatcacggcg aaatgcagat
     4861 cacctcgtca ccgaaaaaat tctccggata cttccacttt taccgcaagc acaaagacaa
     4921 attcgaccgc gccagcaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg
     4981 catgacctgg cgcactttgc tgacgctgct gtcggtgcgt aacggcaaac gtctggctga
     5041 tggtattcgg gggcgctaaa gatgctggaa gatctgcgcg ccaacagctg gagtttacgc
     5101 ccgtgctgca tggttcttgc ctatcgtgcc gctcattttt gttcggtgtg gcgcaaaaag
     5161 aacgtcctca acaatctttg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa
     5221 tgctttttcg gttatgaaat ccaggccgcc gcgaccatag gccgacgctt tactatccat
     5281 cacggttacg ccgtagtgat caataaaaac gtggtcgcgg gggatgattt caccattcgt
     5341 cacggcgtca ctatcggcaa tcgtggcgcc gataacatgg catgtccaca cattggcaac
     5401 ggcgtcgaac tcggtgccaa cgtcattatt cttggtgata tcacgattgg taacaacgtc
     5461 accgtgggcg cgggcagcgt ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga
     5521 gaaaaagcgc gagtgaaggt aattaaatga atattctgca atttaatgtg cgactggcgg
     5581 aaggcggggc agcaggtgtg gcattagatc tccaccagcg cgcgctgcaa caggggctgg
     5641 cgtcacattt tgtctacggc tacggcaaag gcggcaaaga gagcgtcagc catcagaact
     5701 atccgcaggt catcaaacat acgccacgga tgaccgcgat ggcgaacatt gcgttgtttc
     5761 gtctgcttaa tcgcgatctg ttcggcaatt tcaatgagtt atatcgcacc attactcgca
     5821 caccgggccc ggtggttctg cattttcatg tgctgcacag ctactggttg aatcttaaga
     5881 gcgtggtgcg cttttgcgaa aaagtgaaaa accataaacc ggatgtcact ctggtctgga
     5941 cgctacacga ccactggagc gttaccggac gctgcgcctt taccgacggt tgcgaaggct
     6001 ggaaaacggg ctgccagaaa tgcccgacct taaataacta tccaccggtg aagattgatc
     6061 gcgcgcacca gctggtggcg ggtaaacgcc agttattccg cgagatgctg gcactgggct
     6121 gtcagtttat ttcccccagc cagcatgtgg ctgatgcttt caatagtctg tacggtccag
     6181 ggcgttgccg gattatcaat aatggcattg atatggcaac cgaagcgatt ctggcggact
     6241 tgcctccggt acgcgaaacc cagggcaagc cgaaaatcgc ggtggtggcg catgacctgc
     6301 gttacgacgg caaaactaac cagcaactgg tacgcgagat gatggcgctg ggtgacaaaa
     6361 tcgaactgca tacctttggt aagttctcgc cgttcaccgc tggtaacgtt gtcaatcacg
     6421 gctttgaaac tgacaagcgc aagttaatga gcgtgctcaa tcagatggat gcgctggtgt
     6481 tcagttctcg cgtcgataac tacccgctga ttttgtgtga ggcgttatcg attggcgtgc
     6541 cggtgatcgc cacccatagc gatgcggcgc gggaagtgct gcaaaaatcc ggtggtaaaa
     6601 ccgtcagcga agaagatgtg ctgcaactgg tgcagttaag caaaccggaa atcgcacagg
     6661 cgatatttgg taccacgctg gctgaattca gccagcgcag ccgcgccgcc tacagtggac
     6721 aacagatgct ggaggagtat gtcaacttct atcagaatct gtagctacct gctgctgccg
     6781 ctgatctacc tgctggttaa cgtcaaaatc gcccagcttg gcgaaagttt ccccatcacc
     6841 atcgtcactt ttttacctgt cctgttactg ctgtttttag agcgcatcag cgttaaaaaa
     6901 ttgatgattg ccttggggat tggcgcggga cttacggcgt ttaactacct gtttggtcag
     6961 tcgctggatg ccagtaaata cgtcacttca accatgctgt ttgtctatat tgtgatcatt
     7021 attggcatgg tgtggagtat tcgctttaaa acgatttcgc cacacaacta tcgcaagata
     7081 ttacgtttct tttatctggt ggtcgggctg gtggtggtgc tggcggcggt ggagatggcg
     7141 caaattatcc tcaccggcgg tagcagtatt atggagtcga tttcgaaata tctgatttac
     7201 agcaacagct atgtgctgaa tttcattaaa ttcggcggca agcgcacgac agcactttat
     7261 ttcgaaccgg catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt
     7321 ggtatcaaaa cgcctaaaac agatgctatg attctcgcag ggataatatt atccggttca
     7381 ttttcagggg ttatgacctt tatccttttt tatttgctgg agtgggcatt tcaatatctg
     7441 aataaagagg cgattaagaa aaagttaccg ttagcattga tttctctggc tgtattcctg
     7501 gttggtgtgg taatcgcgtt cccttatatt tccacccgtc tgggcgattt aggtacagaa
     7561 ggatcgtcat cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttaacc
     7621 catattgacg gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata
     7681 tttaacggtg cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat
     7741 ttttcctggt tcgcagtgtt tttatcgctg tggtacatgg gaaaagtgat aaaaatgatg
     7801 atcaacgctt ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggta
     7861 tcgctgtttt ttaccggttc gatatttagc ccagaatatg cgtttttaat cgtctgcccg
     7921 tttattttgc gaaaagcgtt aaatatacac cctaaataat tcgagttgca ggaagtgggt
     7981 gagcgaggaa aaccaacgca catgcagctt gaagtatgac gggtattacg aggtaaaaca
     8041 tgttgcttag cataatcact gtcgcgtttc gtaacctcga aggaatagtc aaaacacacg
     8101 cctcgctggc gcatctggcg caggcggacg atatcagctt cgaatggatt gttgttgatg
     8161 gcggttccaa cgacggcacc cgtgagtatc tggaaaatct caatggtatc tataacttac
     8221 actttgtcag cgaaccagat aacggtatct acgacgccat gaacaaaggt attgaaatgg
     8281 cgcaaggcaa gttcgcgttg tttctcaact cgggcgatat ttttcatcag gatgccgcat
     8341 attttgtccg taagttaaaa gtgcaaaaag ataacgtgat gatcaccggc gatgcgctgc
     8401 tggattttgg cgacgggcat aaaattaaac gtagcgccaa accgggttgg tatatttacc
     8461 acagcctgcc cgccagtcat caggcgatat ttttcccggt gtccggcttg aagaaatggc
     8521 gttatgacct ggagtataaa gtttcttccg attacgcgct ggcggccaaa atgtataaag
     8581 ccggttatgc atttaaaaaa ctcaatggcc tggtgtctga attttctatg ggtggggtat
     8641 ctaccaccaa taatatggaa ttgtgtgctg acgccaaaaa agtccaacgg caaatattgc
     8701 acgtgcctgg cttttgggct gaattatcct ggcatttacg ccaacgtacg acctcaaaga
     8761 cgaaagcctt atataacaaa agctgaatat aagggaaaac gatgcaggat ttaagtggat
     8821 tctcggtgcc gaaagggttc cggggtggca acgctattaa agtgcaattg tggtgggcag
     8881 tacaggcaac aatatttgcc tggtcgccac aagtattgta tcgctggcgg gcatttttat
     8941 tacgtttatt cggcgcaaaa ataggaaaaa acgtagtcat tcgtccgtca gtaaaaatta
     9001 cctatccgtg gaaattaacg ttaggtgatt acgcgtgggt cggcgatgag gtcaatttat
     9061 ataccctcgg tgaaataacc attggcgcac attcggtgat atcgcaaaaa agttatttat
     9121 gcactggtag ccacgaccat gcaagtcaac atttcaccat taacgccacg cctattgtga
     9181 ttggcgagaa atgctggctg gcaaccgatg tctttgttgc ccctggcgtc acgatcggcg
     9241 acggcaccgt cgtgggtgcg cgaagcagtg tttttaaatc gcttccggca aatgtggttt
     9301 gtcgggggca ccccgcagtg gtgatacgca aacgcgttga aactgaataa attcaaaaaa
     9361 tacagaggaa taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg
     9421 ttcttacctg gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg
     9481 tgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatccgc acacctgcaa
     9541 cccgaaattc catctgcatt atggcgacct gagtgatacc tccaacctga cgcgcatttt
     9601 gcgtgaagtg cagccggatg aagtgtacaa cctgggggca atgagccacg ttgcggtttc
     9661 ttttgagtca ccggaatata ccgcagacgt cgatgcgatg ggtacgctgc gcctgctcga
     9721 ggcgatccgc ttcctcggtc tggaaaagaa aacccgtttc tatcaggctt ctacttctga
     9781 actgtacggt ctggtgcagg aaattccgca gaaagagacc acgccgttct acccgcgatc
     9841 tccgtatgcg gttgccaaac tgtacgccta ctggatcacc gttaactacc gcgaatccta
     9901 cggcatgtac gcctgtaacg gtattctctt caaccatgaa tccccgcgcc gtggcgagac
     9961 tttcgttacc cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg
    10021 cctgtatctc ggcaatatgg attccctgcg tgactggggc cacgccaaag actacgtaaa
    10081 aatgcagtgg atgatgctgc aacaggaaca accggaagat ttcgttattg ccaccggcgt
    10141 tcagtactcc gtgcgtcagt tcgtggaaat ggcggcggca caactgggca tcaaactgcg
    10201 ctttgaaggc acgggtgttg aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc
    10261 gccgggcgtt aaaccgggtg atgtgattat cgccgttgac ccgcgctact tccgtccggc
    10321 agaagttgaa acgctgctcg gtgacccgac caaagcgcac gaaaaactgg gctggaaacc
    10381 ggaaatcacc ctcagagaga tggtgtctga aatggtggct aatgaccttg aagcggcgaa
    10441 aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc
    10501 atgagtaaac aacgcatttt tattgctggc catcgcggga tggtcggttc cgccatcagg
    10561 cggcagctcg aacagcgcgg tgatgtggag ctggtattac gcacccgcga tgaactgaac
    10621 ctgctggaca gccgcgcggt gcatgatttc tttgccagcg aaagcattga ccaggtctat
    10681 ctggcggcgg cgaaagtggg tggtattgtt gccaacaaca cgtatccggc agatttcatc
    10741 taccagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa cgacgtgaac
    10801 aaactgctgt ttctcggatc gtcctgcatc tacccgaaac tggcaaaaca gccgatggca
    10861 gaaagcgagt tgttgcaggg cacgctggag ccgaccaacg agccttacgc cattgccaaa
    10921 atcgccggga tcaaactgtg tgaatcttac aatcgccagt acggacggga ttaccgctca
    10981 gtcatgccaa ccaacctgta cgggccacac gacaacttcc acccgagtaa ttcgcatgtg
    11041 atcccagcat tgctgcgtcg cttccatgag gcgacggcac agaatgcgcc ggacgtggtg
    11101 gtgtggggca gcggtacgcc gatgcgcgaa tttctacacg ttgatgatat ggcagcggcg
    11161 agcattcatg tcatggagct ggcacacgaa gtctggcagg agaacaccca gccgatgctg
    11221 tcgcacatta acgtcggcac gggcgttgac tgcactatcc gtgagctggc gcaaaccatc
    11281 gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc ggatggcacg
    11341 ccgcgcaaac tgctggatgt gacgcggctg catcagcttg gctggtatca cgaaatctca
    11401 ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcaaga ccgctttcgg
    11461 gggtaatgat gtttttacgt caggaagact ttgccacggt agtgcgctcc actccgcttg
    11521 tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc aaaagaacca
    11581 accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc
    11641 tggaagccgc atttgagcgg ctgacgatgg cggaactggg gttgcgtctg ccgataacag
    11701 caggccagtt ttacggtgtc tggcagcact tttatgacga taacttctcc ggctcggatt
    11761 ttaccactca ctatgtggtg ctcggttttc gcttcagagt agcggaggaa gagctgttac
    11821 tgccggatga gcagcatgac gattaccgct ggctgacgcc ggacgcgttg ctcgccagtg
    11881 acaatgttca cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg
    11941 gattatgaaa atactggtct acggcattaa ctactcgccg gagttaaccg gcatcggcaa
    12001 atacaccggc gagatggtgg aatggctggc ggcacaaggt catgaggtgc gggtcattac
    12061 cgcgccgcct tactacccgc agtggcaggt gggcgagaac tattccgcct ggcggtacaa
    12121 acgggaagag ggggccgcca tggtgtggcg ctgcccgctg tacgtgccaa aacagccgag
    12181 cactctgaaa cgcctgttgc atctcggcag ttttgccgtc agcagtttct ttccactgat
    12241 ggcgcaacgt cgctggaagc cggatcgcat tatcggcgta gtgccaacgc tgttttgcac
    12301 gccgggaatg cgcctgctgg cgaaactctc tggtgcgcgt accgtgctgc atattcagga
    12361 ttacgaagtg gacgccatgc tggggctggg ccttgccgga aaaggcaaag gcggcaaagt
    12421 ggcacagctg gcgacggcgt tcgaacgtag cggactgcat aacgtcgata acgtctccac
    12481 gatttcgcgt tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg aaaacgtcat
    12541 tttcttccca aactggtcgg aaatcgcccg ttttcagcat gtcgcagacg ccgatgttga
    12601 tgcccttcgt aaccagcttg gcctgccgga taacaaaaaa atcattcttt actccggcaa
    12661 tattggtgaa aagcaggggc tggaaaacgt tattgaagct gccgatcgcc tgcgcgatga
    12721 accgcttatt tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc
    12781 gcagcagcgt ggactgcgca acatgcaatt ttttccgctg caatcgtatg acgctttacc
    12841 cgcactgctg aagatggggg attgccatct ggtggtgcaa aaacgcggcg cggcagatgc
    12901 cgtattgccg tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg tgattaccgc
    12961 agaagcccac accgaactgg ggcagctttg cgaaaccttt ccgggcattg cggtttgcgt
    13021 ggaaccggaa tcggtcgagg cgctggtggc ggggattcgt caggcgctcc tgctgcccaa
    13081 acacaacacg gtggcacgtg aatatgccga acgcacgctc gataaagaga acgtgttacg
    13141 tcaatttata aatgatattc ggggataatt atggcgcagt cgaaactcta tccagttgtg
    13201 atggcaggtg gctccggtag ccgcttatgg ccgctttccc gcgtacttta ccccaagcag
    13261 tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg cctgaacggc
    13321 gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat tgtcgcggaa
    13381 cagctgcgtc aactaaataa actcaccgag aacattattc tcgaaccggc ggggcgtaac
    13441 accgcacctg ccattgcgct ggcggcgctg gcggcaaaac gtcatagccc ggagaacgac
    13501 ccattaatgc tggtgctggc ggcggatcat gtgattgccg atgaagacgc gttccgtgcc
    13561 gccgtgcgta atgccatgcc gtatgccgaa gcgggcaagc tggtgacctt cggcattgtg
    13621 ccggatctac ctgaaaccgg atatggctat attcgtcgcg gtgaagtgtc ggcgggtgag
    13681 caggatacgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct ggaaaccgcg
    13741 caggcctatg tggcaagtgg cgaatattac tggaacagcg gtatgttcct gttccgtgcc
    13801 ggacgttatc tcgaagaact gaaaaagtat cgtccggata ttctcgatgc ctgtgaaaaa
    13861 gcgatgagcg ccgtcgatcc ggatctcgat tttattcgtg tggatgaaga ggcgtttctc
    13921 gcctgtccgg aagagtcggt agattacgcg gtcatggaac gcacggcaga tgccgttgtg
    13981 gtgccgatgg atgcgggctg gagtgatgtc ggttcctggt cctctttatg ggagatcagt
    14041 gcccacaccg ccgagggcaa cgtttgccat ggcgatgtga ttaatcacaa aactgaaaac
    14101 agctatgtgt atgccgaatc tggcctggtc accaccgtcg gggtgaaaga tttggtggta
    14161 gtgcagacca aagatgcagt gctgattgct gaccgtaacg cggtgcagga tgtgaaaaaa
    14221 gtggtcgagc agatcaaagc tgatggtcgc catgagcatc gggtgcatcg cgaagtgtat
    14281 cgtccgtggg gcaaatatga ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc
    14341 accgtgaaac cgggcgaagg gttgtcggta cagatgcatc atcaccgcgc ggaacactgg
    14401 gtggttgtcg cgggaacggc aaaagtcact atcgacggtg atatcaaact gcttggtgaa
    14461 aacgagtcca tttatattcc gctgggggcg acgcattgcc tggaaaaccc ggggaaaatt
    14521 ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga tgtggtgcgc
    14581 ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg caatgaatgc gaaaccgcga
    14641 tgtaaataac gacaaaaaca aaattggccg tttcggtcag ggccaactat tgcctgaaaa
    14701 agggtaacga tatgaaaaaa ttaacctgct ttaaagccta tgatattcgc gggaaattag
    14761 gcgaagaact gaatgaagat atcgcctggc gcattggtcg cgcctatggc gaatttctca
    14821 aaccgaaaac cattgtgtta ggcggtgatg tccgcctcac cagcgaaacc ttaaaactgg
    14881 cgctggcgaa aggtttacag gatgcgggcg tcgacgtgct ggatatcggc atgtccggca
    14941 ccgaagaaat ctatttcgcc acgttccatc tcggcgtgga tggcggcatt gaagttaccg
    15001 ccagccataa cccgatggat tacaacggca tgaagctggt gcgcgaaggg gctcgcccga
    15061 tcagcggcga taccggactg cgcgacgtcc agcgtctggc agaagccaac gactttcctc
    15121 ccgtcgatga aaccaaacgc ggtcgctatc agcaaatcaa tctgcgtgac gcttacgttg
    15181 atcacctgtt cggttatatc aacgtcaaaa acctcacgcc gctcaagctg gtgattaact
    15241 ccgggaacgg cgcggcgggt ccggtggtgg acgccattga agcccgcttt aaagccctcg
    15301 gcgcaccggt ggagttaatc aaagtgcata acacgccgga cggcaatttc cccaacggta
    15361 ttcctaaccc gctactgccg gaatgtcgcg acgacacccg caatgcggtc attaaacacg
    15421 gcgcggatat gggcattgct tttgatggcg attttgaccg ctgtttcctg tttgacgaaa
    15481 aagggcagtt tatcgagggt tactacattg tcggtttgct ggcagaagcg ttcctcgaaa
    15541 aaaatcccgg cgcgaagatc atccacgatc cacgtctctc ctggaacacc gttgatgtgg
    15601 tgactgccgc aggcggcacc ccggtaatgt cgaaaaccgg acacgccttt attaaagaac
    15661 gtatgcgcaa ggaagacgcc atctacggtg gcgaaatgag cgcccaccat tacttccgtg
    15721 atttcgctta ctgcgacagc ggcatgatcc cgtggctgct ggtcgccgaa ctggtgtgtc
    15781 tgaaaggaaa aacgctgggc gaactggtgc gcgaccggat ggcggcattc ccggcaagcg
    15841 gtgagatcaa cagcaaactg gcgcaacccg ttgaggcgat taaccgcgtg gaacagcatt
    15901 ttagccgtga ggcactggcg gtggatcgta ccgatggcat cagcatgacc tttgccgact
    15961 ggcgctttaa tctgcgctcc tccaacaccg aaccggtggt gcggttgaat gtggaatcac
    16021 gcggtgatgt gccgctgatg gaagcgcgaa cgcgaactct gctgacgttg ctgaacgagt
    16081 aatgtcggat cttcccttac ccccactgcg ggtaaggggc taaaaacagg aacaacgatg
    16141 acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa
    16201 cgcttttcag atatcaccat catgtttgcc gggctatggc tggtttgcga agtgagcgga
    16261 ctgtcattcc tctacatgca cctgttggtg gcgctgatta cgctggtggt gttccagatg
    16321 ctgggcggca tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc gacagaattt
    16381 gccctgttgc tacaaaactg gacgttaagc gtgattttca gcgccggact ggtggcgttc
    16441 aacaatgatt tcgacacgca attgaaaatc tggctggcgt ggtatgggct gaccagcatc
    16501 ggactggtgg tttgccgttc atgtattcgt attggggcgg gctggctgcg taatcatggc
    16561 tataacaagc gcatggtcgc ggtggcgggg gatttagccg ccggacaaat gctgatggag
    16621 agcttccgta atcagccgtg gttagggttt gaagtggtgg gcgtttacca cgacccgaaa
    16681 ccgggtggcg tttctaacga ctgggcgggt aatctgcaac agctggtcga ggatgcgaaa
    16741 gcgggcaaga ttcataacgt ctatatcgcg atgcagatgt gcgacggcgc gcgagtgaaa
    16801 aaactggtcc atcaactggc ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt
    16861 accttcaaca ttctccattc ccgtatcgaa gagatgaacg gcgtaccggt ggtgccgctg
    16921 tatgacacgc cgctttccgg ggttaaccgc ctgataaaac gtgcggaaga tattgtgctg
    16981 gcgacgctta tcctgctgct gatctccccg gtgctgtgct gcattgcgct ggcggtgaaa
    17041 ctgagctcac cggggccggt tattttccgc cagactcgct acggcatgga tggcaagccg
    17101 atcaaagtgt ggaagttccg ttccatgaaa gtgatggaga acgacaaagt ggtgacccag
    17161 gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg
    17221 gacgaattgc cgcagtttat caatgtgctg actggcggga tgtcgattgt cggtccgcgt
    17281 ccgcacgcgg tggcgcataa cgaacagtat cgccagctca ttgaaggcta catgctgcgc
    17341 cataaagtga aaccgggcat taccggctgg gcgcagatta acggctggcg cggcgaaacc
    17401 gacacgctgg agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg
    17461 agcgtctggt tcgatatcaa aatcgttttc ctgacggtat tcaagggctt cgttaacaaa
    17521 gcggcatatt gatatgagct tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat
    17581 tgccacggtg atcatcatcg gcctcggtct ggtgcaaatg actgtgctgg cgcggattat
    17641 agataaccac cagttcggtt tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac
    17701 gctttctgac ttcggtatcg ctaactccat tattcagcga aaagaaatca gccaccttga
    17761 actcaccacg ctgtactggc tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt
    17821 tttgttgagt gatctcatcg gcgacttgct gaataacccg gacctggcac cgttgattaa
    17881 aacattgtcg ctggcgtttg tggtgatccc ccacgggcaa cagttccgcg cgttgatgca
    17941 aaaggagctg gagttcaaca aaatcggcat gatcgaaacc agcgcggtgc tggcgggctt
    18001 tacttttacg gtggttagcg cccatttctg gccgctggcg atgaccgcga ttctcggtta
    18061 tctggtcaat agtgcggtga gaacgctgct gtttggctac tttggccgca aaatttatcg
    18121 ccccggtctg catttctcgc tggcgtcggt ggcaccgaac ttacgctttg gtgcctggct
    18181 gacggcggac agcatcatca actatctcaa caccaacctt tcaacgctgg tgctggcgcg
    18241 tattctcggc gcgggcgtgg ctgggggata caacctggcg tacaacgtgg ccgttgtgcc
    18301 accgatgaag ctgaacccga tcatcacccg cgtgttgttt ccggcgttcg ccaaaattca
    18361 ggacgataca gaaaagctgc gcgtcaactt ctacaagctg ctgtcggtgg tggggattat
    18421 caactttccg gcgctgctgg ggctaatggt ggtgtcgaat aactttgtac cgctggtatt
    18481 tggcgaaaag tggagcagca ttattccggt gctgcaattg ctgtgtattg tggggctact
    18541 gcgctccgtg ggtaacccga ttggttcgct gctgatggcg aaagcgcggg tcgatatcag
    18601 ctttaaattc aacgtattca aaacctttct gtttattccg gcaatcatca ttggtgggca
    18661 gatggcgggc gcaatcggcg tcacgcttgg cttcctgctg gtgcagatta tcaacaccat
    18721 tctgagctat ttcgtgatga ttaaaccggt gctcggttcc agttatcgcc agtacatcct
    18781 gagtttgtgg ctgccgtttt atctctcgct gccgacgctg gtggtcagtt atgcgctggg
    18841 cattgtgctg aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa tagccacggg
    18901 cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa
    18961 gcgtcagttt tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt gaatggttgt
    19021 tttcggttcc ggcaacggtt gccggatgcg gcgtaaaacg ccttatccgt cctggggggc
    19081 tgggcttgga tcttgtaggt ctgataagac gcgttagcgt agcatcaggc actgaatgcc
    19141 ggatgcggcg tgaacgcctt atccggccta cggttctgtg ctcggatctt gtaggcctga
    19201 taagacgcgt cagcgtcgca tcaggcactg aatgccggat gcggcgtaaa acgccttatc
    19261 cgtcctacgg ctctgtgctc gggtcttgta ggcctgataa gacgcgtcag cgtcgcatca
    19321 ggcactgaat gccggatgcg gcgtgaacgc cttatccgtc ctacggttct gtgctcggat
    19381 cttgtaggcc tgataagacg cgtcagcgtc gcatcaggca aaaaacagca atgaaaacaa
    19441 caaacacagc ctcacctggc atcaaattta tgaggaaaaa atgaaattac ttattctggg
    19501 caaccacact tgcggcaatc gcggcgacag cgccatcctg cgcggcttac ttgatgccat
    19561 caatatcctc aatccacatg ccgaagtgga cgtgatgagt cgctatccgg tcagttcttc
    19621 ctggctgctc aaccgcccgg taatgggcga tccgctgttc ctgcaaatga aacaacacaa
    19681 cagtgcagcg ggcgttgtcg ggcgcgttaa aaaagtcctc cgtcgccgtt accaacatca
    19741 ggtactgctc tcacgcgtca ccgacactgg caagttgcgt aatatcgcca tcgcccaggg
    19801 attcactgac ttcgtgcgcc tgctgtcagg ttacgacgcc attattcagg tcggcggatc
    19861 gttttttgtc gatctctatg gcgtgccgca gtttgaacat gcgctttgca cgtttatggc
    19921 gaaaaagccg ctgtttatga ttggtcacag cgtcggcccg ttccaggata agcaatttaa
    19981 ccaactggcg aactacgttt ttggtcactg cgacgcgctg atcctgcgcg aatcggtcag
    20041 ccttgatttg atgaaacgca gcaatatcac taccgcaaaa gttgaacatg gcgtcgatac
    20101 cgcgtggctg gtcgatcacc acacagaaga cttcaccgcc agctatgctg ttcaacactg
    20161 gctggacgtt gccgcacaac agaaagcggt ggccattacc ctgcgtgaac tggcaccgtt
    20221 cgacaaacgc ctcggcacca ctcaacaagc gtatgaaaaa gcctttgccg gggtggtcaa
    20281 tcgcattctc gacgaaggct atcaggtgat tgcgctctcc acctgtacgg gcatcgacag
    20341 ctataacaaa gacgaccgca tggtggcgct caatctgcgc cagcacatca gcgatcctgc
    20401 ccgttaccac gtggtgatgg atgaactcaa cgatctggaa atgggcaaaa ttctgggggc
    20461 ctgtgaactc accgtcggta cgcgcctgca ctccgccatt atctcaatga actttgccac
    20521 cccggcgatt gccatcaact atgaacataa atccgccggg atcatgcagc agctgggact
    20581 accggagatg gcgattgata tccgtcattt attagacggc agcctgcaag cgatggttgc
    20641 ggatacctta ggccaacttc cggcgctgaa cgcacgactt aacgaagccg tcagtcgcga
    20701 acgtcagaca ggaatgcaga tggtgcagtc tgtgcttgag cgcatcgggg aggtgaaatg
    20761 aaggtcggct tctttttatt gaaatttccg ctgtcgtcgg aaaccttcgt cctcaaccaa
    20821 atcactgcgt ttattgatat gggctttgag gtggagattg tcgcgttgca aaaaggcgac
    20881 acacaaaaca cccacgcggc atggacgaaa tacaaccttg ccgccagaac ccgctggtta
    20941 caggacgaac ctacgggcaa agtggcgaag ctgcgccacc gagccagcca gaccttacgc
    21001 ggcattcatc gtaaaaatac ctggcaggcg ctcaacctca aacgctatgg cgcagagtcg
    21061 cggaacctga ttttgtctgc catttgtggt caggtcgcaa catcgttaca tgccgatgtc
    21121 tttatcgcac attttggccc tgcgggggta accgcagcaa aactccgcga actgggtgtc
    21181 attcgcggca aaattgccac tatcttccac ggcattgata tctccagtcg ggaggtgctc
    21241 aaccattaca ctcccgaata tcagcaactg tttcgccgtg gcgacctgat gctaccaata
    21301 agcgatctgt gggccggaag gctgcaaaaa atgggctgcc cgagggaaaa aatcgccgta
    21361 tcgcgcatgg gcgtggacat gacgcgcttt agcccgcgtc cggtgaaagc gcctgcaacg
    21421 ccgctggaaa tcatttccgt cgcacgctta accgagaaaa aaggcctgca tgtggcgatt
    21481 gaagcctgcc gtcagttgaa agagcagggc gtggcatttc gctatcgcat cctcggcatt
    21541 ggcccgtggg aacgacgcct gcgcaccctc atcgaacaat atcaactgga agatgtggtg
    21601 gagatgccgg gctttaaacc gagccatgaa gtgaaagaga tgctcgatga cgcggatgtc
    21661 ttcctgttgc catcggttac gggtgcggat ggcgatatgg aaggtattcc ggtggcgcta
    21721 atggaagcga tggcggtcgg tattccggtg gtttctactc tgcatagcgg aattcctgaa
    21781 ctggtggagg ctgacaaatc cggctggctg gtgcctgaga acgatgctcg cgcactggcg
    21841 caacgactgg cggcgtttag ccaactggac agcgacgaac tggctcctgt cgtcaaacgt
    21901 gctcgcgaaa aagtcgaaca cgattttaac cagcaggtga tcaatcgaga actcgccagc
    21961 ttgctgcagg ctttatagag gagaacgcat gccatttaaa aaactctccc gacgcacctt
    22021 cctgacggca agttctgtgc ttgccttcct ccatacgcct ttcgcccgcg cacttcccgc
    22081 tcaacaaagc gttaacatta acgactacaa ccctcacgac tggatcgcct catttaaaca
    22141 agccttcagc gaagggcaaa cggtcgtcgt gcccgccgga ttggtttgtg acaatatcaa
    22201 caccggcatc ttcattcctc ctggcaaaac gttacacatc cttggaagcc tgcgcggcaa
    22261 cggcaaaggg cgatttgtct tacaggacgg cagccaggtg acaggggagg ggggcggcag
    22321 tatgcataac atcaccctgg atgtgcgcgg ctctgactgc accatcaaag ggctggcgat
    22381 gagcggcttt ggtccggtaa cgcagattta tatcggcggc aaaaacaaac gggtcatgca
    22441 caacctgacc atcgataacc tgaccgttag ccacgctaat tacgccatct tacgccaggg
    22501 atttcataac cagataatcg gtgccaacat caccaactgt aagttcagcg acttacaggg
    22561 cgacgctatt gaatggaacg tggcgattaa cgacagtgat attttgatat ccgaccatgt
    22621 catcgagcgc atcaactgta ctaatggcaa aatcaactgg ggcattggca taggccttgc
    22681 gggaagcact tatgataata attacccaga agatcaggca gtgaaaaact ttgtcgtggc
    22741 gaatatcacg ggatcggatt gtcggcaact gatccatgtt gaaaatggta aacattttgt
    22801 tatccgtaat atcaaagctc gtaatatcac gccggatttc agtaagaaag caggtattga
    22861 taacgccaca gttgctattt atggttgtga caactttgtg attgataata ttgaaatgat
    22921 taatagtgcc gggatgttaa tcggctatgg ggtaattaaa ggcaaatatc tctcgatacc
    22981 gcagaatttc cgagtgaatg atattcaatt ggataatact catcttgctt ataaattgcg
    23041 cggcatccaa atctccgccg ggaatgctgt ctcctttgtg gcgctgacta acattgagat
    23101 gaagcgtgcg tcgctggagt tacacaacaa accgcaacat ctttttatgc gtaatatcaa
    23161 tgtgatgcag gaatcctcag ttggaccagc attgagcatg aactttgaca tgcgcaaaga
    23221 cgttcgaggc gtgtttatgg cgaaaaaaga aacactgctg tctcttgcaa atgttcatgc
    23281 ggtgaatgaa aaagggcaaa gctccgtcga tatcgacagg attaatcacc atattgttaa
    23341 tgtggaaaag attaacttta gattgccgga acggagagag tagatttgcg accattcctg
    23401 gaaaaatgga gtcatactta ggaacaatgc tactgcaatc cacaacgaag cggcgtaaca
    23461 tcacaagtaa ttcagtaatc aattcagggt aattgatgct ggcgaaaaaa atcgaacaag
    23521 ctataattca gcaaccattt tacaggtgga tgaaataatg acgaatttaa aagcagttat
    23581 tcctgtagcg ggtctaggga tgcatatgtt gcctgccact aaggcgattc ccaaagagat
    23641 gctaccgatc gtcgacaagc caatgattca gtacattgtt gacgagattg tggctgcagg
    23701 gatcaaagaa atcctcctgg taactcatgc gtccaagaac gcggtcgaaa accacttcga
    23761 tacatcttat gaattagaat ctctccttga actgcgcgtg aagcgtcaac tgctggcgga
    23821 agtacagtcc atctgtccgc cgggcgtgac cattatgaac gtgcgtcagg gcgaaccttt
    23881 aggtttaggc cactccattt tgtgtgcgcg acctgccatt ggtgacaacc catttgtcgt
    23941 ggtactgcca gacgttgtga tcgacaatgc cagcgccgac ccgctacgtt acaaccttgc
    24001 tgccatgatt gcacgtttca acgaaacggg ccgcagccag gtgctggcaa aacgtatgcc
    24061 gggtgacctc tctgaatact ccgtcattca gactaaagag ccgctggatc gtgaaggtaa
    24121 agtcagccgc attgttgaat ttatcgaaaa accggatcag ccgcagacgc tggactcaga
    24181 catcatggcc gtaggtcgct atgtgctttc tgccgatatt tggccggaac tggaacgtac
    24241 tcagcctggt gcatggggac gtattcagct gactgatgcc attgctgagt tggcaaaaaa
    24301 acaagcagtt gacgcaatgc tgatgactgg ggacagttac gactgcggaa agaaaatggg
    24361 ttatatgcaa gcgtttgtga agtatgggct gcgtaacctg aaggaagggg cgaagttccg
    24421 taaaggtatt gagaagctgt taagcgagta acaaattcct gactggaagt gtcggtcagt
    24481 aagtaattaa aaacggcagt gaagattcat agcataagtt ttgctcattg aatctcactg
    24541 ccgttgtctt ttttataagt acaataaaaa cagcatgtta gcttgcaatc atagttcaaa
    24601 tttttccagg attttccttg tttctacagc tgtttggtaa gacaattagc gtttgaattt
    24661 ttcggatttg gcacgagtgg gttacgctcg tcacatcgtg gaaatgtacg cagtgctctg
    24721 gtagcggtta agccaggggc ggtagcgtgc attaatgcat ctattaatca aatctggagc
    24781 agtctatttc acagcatgct ctctggctat atggaataaa aaagtgaaga tacttgttac
    24841 tggtggcgca ggatttattg gttctgctgt tgttcgtcac ataataaata atacgcaaga
    24901 tagtgttgtt aatgtcgata aattaacata cgccggaaac ctggaatcac ttgcagatgt
    24961 ttctgattct gaacgctatt tctttgaaca tgcggatatt tgtgatgcag ctgcaatggc
    25021 acggattttt gctcagcatc agccggatgc agtgatgcac ctggcagctg aaagccatgt
    25081 tgaccgttca attacaggcc ctgcggcatt tattgaaacc aatattgtgg gtacttatgt
    25141 ccttttagaa gcggctcgga attattggtc tggtctggat gatgaaaaga aaaaaaactt
    25201 ccgttttcat catatttcta ctgatgaggt gtatggtgac ttaccccatc cggatgaagt
    25261 aaatagcaat gaaacgttgc cgctatttac ggaaacgaca gcatacgcgc caagtagtcc
    25321 atattctgct tctaaagctt ccagcgatca tttggttcgc gcatggaaac gtacttatgg
    25381 tttaccgacc attgtgacta attgctcgaa caactatggt ccttatcatt tcccggaaaa
    25441 gcttattcca ctggttattc ttaattcact ggaaggtaag gcattaccta tttatggcaa
    25501 aggagatcag atccgcgact ggttgtatgt agaggatcat gctcgagcgt tatataccgt
    25561 cgtaaccgaa ggtaaagcgg gcgaaactta taacattggt ggacacaacg aaaagaaaaa
    25621 catcgacgta gtgttcacta tttgtgattt gttggatgag atagtcccga aagagaaatc
    25681 ttaccgcgag caaattactt atgttaccga tcgtccggga cacgatcgcc gttatgcgat
    25741 tgatgctgag aagattggtc gcgaattggg atggaaacca caggaaacgt ttgagagtgg
    25801 gattcgtaaa acggtggaat ggtacctgtc caatacaaaa tgggttgata atgtgaaaag
    25861 tggtgcctat caatcgtgga ttgaacagaa ctatgagggc cgccagtaat gaatatcctc
    25921 ctttttggca aaacagggca ggtaggttgg gaactacagc gtgctctggc acctctgggt
    25981 aatttgattg ctcttgatgt tcactccact gattactgtg gtgattttag taatcctgaa
    26041 ggtgtagctg aaaccgtaag aagcattcgg cctgatatta ttgtcaacgc agccgctcac
    26101 accgcagtag acaaagcaga atcagaaccg aagtttgcac aattactgaa cgcgacgagt
    26161 gtcgaagcga tcgcgaaagc agccaatgaa gtcggcgcct gggttattca ctactctact
    26221 gactacgtat ttccggggac cggtgaaata ccatggcagg aggaggatgc aaccgcaccg
    26281 ctaaatgttt acggtgaaac caagttagcg ggagaaaaag cattacaaga gcattgtgcg
    26341 aagcacctta ttttccggac cagctgggtc tatgcaggta aaggaaataa cttcgccaaa
    26401 acaatgttgc gtctggcaaa agagcgtgaa gaattagccg ttattaatga tcagtttggt
    26461 gcgccaactg gcgcagagtt actggctgat tgtacggcac atgctattcg tgtggcactg
    26521 aataaaccgg aagtcgcagg cttgtaccat ctggtagcta gtggtaccac aacgtggcac
    26581 gattatgctg cgctggtttt tgaagaggcg cgcaaagcag gcattcccct tgcactcaac
    26641 aagctcaacg cagtaccaac aacagcctat cctacaccag ctcgtcgtcc acataactct
    26701 cgccttaata cagaaaaatt tcagcagaac tttgcgcttg tcttgcctga ctggcaggtt
    26761 ggcgtgaaac gaatgcttaa cgaattattt acgactacag caatttaata gtttttgcat
    26821 cttgttcgta atggtggagc aagatgtatt aaaaggaatg atgaaatgaa aacgcgtaaa
    26881 ggtattattt tggcgggtgg ttctggtact cgtctttatc ctgtgacgat ggccgtcagt
    26941 aaacagctgt taccgattta tgataaaccg atgatctatt acccgctctc tacactgatg
    27001 ttagcgggta ttcgcgatat tctgattatc agtacaccac aggatactcc tcgttttcaa
    27061 caactgctgg gtgacgggag ccagtggggc ctgaatcttc agtacaaagt gcaaccgagt
    27121 ccggatggtc ttgcgcaggc gtttattatc ggtgaagagt ttattggtgg tgatgattgt
    27181 gctttggtac ttggtgataa tatcttctac ggccacgacc tgccgaagtt aatggacgta
    27241 gctgttaaca aagaaagtgg tgcaacggta tttgcctatc acgttaatga tcctgaacgt
    27301 tatggtgtcg tggagtttga taataacggt actgcaatta gcctggaaga aaaaccgctg
    27361 gaaccaaaaa gtaactatgc ggttactggg ctttatttct atgacaatga cgttgtggaa
    27421 atggcgaaaa accttaagcc ttctgcccga ggtgaactgg aaattaccga tattaaccgt
    27481 atttatatgg aacaaggacg tttgtctgtc gctatgatgg ggcgtggcta tgcatggctg
    27541 gatacaggga cgcatcaaag tcttattgaa gcaagcaact tcattgccac cattgaagag
    27601 cgccagggac taaaggtttc ctgtccggaa gaaattgctt atcgtaaagg gtttattgat
    27661 gctgagcagg taaaagtatt agccgaaccg ttgaagaaaa atgcttatgg tcagtatctg
    27721 ctcaaaatga ttaaaggtta ttaataagat gaacgtaatt aaaactgaaa ttcctgatgt
    27781 gctgattttt gaaccaaaag tttttgggga tgaacgtggc ttcttttttg agagttttaa
    27841 tcagaggatt tttgaagaag cagtaggtcg taaggttgag tttgttcagg ataaccattc
    27901 taagtccagt aaaggtgttt tacgtggtct tcattatcag ttagaacctt atgctcaagg
    27961 aaaactggtg cgctgtgttg ttggcgaggt ttttgatgtt gcggttgata ttcgtaaatc
    28021 gtcacctaca tttgggaaat gggttggggt gaatttgtct gctgagaata agcgtcagtt
    28081 gtggattcct gagggatttg cacatggttt tttggtgctg agtgatttag cagaagtttt
    28141 atataaaacg aatcaatatt atgctccatc acatgaaaaa aatattatat ggaatgacct
    28201 cttgcttaat attaaatggc cgagcacagc actgatcact
//
LOCUS       DACADA010000051        28119 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.052, whole genome shotgun sequence.
ACCESSION   DACADA010000051 DACADA010000000
VERSION     DACADA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.052"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            90..353
                     /gene="yhcN"
                     /locus_tag="ISB17_002142"
     CDS             90..353
                     /gene="yhcN"
                     /locus_tag="ISB17_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAP1980075.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(408..680)
                     /locus_tag="ISB17_002143"
     CDS             complement(408..680)
                     /locus_tag="ISB17_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980076.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(772..2739)
                     /gene="aaeB"
                     /locus_tag="ISB17_002144"
     CDS             complement(772..2739)
                     /gene="aaeB"
                     /locus_tag="ISB17_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAP1980077.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPCMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2745..3677)
                     /gene="aaeA"
                     /locus_tag="ISB17_002145"
     CDS             complement(2745..3677)
                     /gene="aaeA"
                     /locus_tag="ISB17_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAP1980078.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3685..3888)
                     /locus_tag="ISB17_002146"
     CDS             complement(3685..3888)
                     /locus_tag="ISB17_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAP1980079.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4071..5000
                     /gene="aaeR"
                     /locus_tag="ISB17_002147"
     CDS             4071..5000
                     /gene="aaeR"
                     /locus_tag="ISB17_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAP1980080.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5122..6567)
                     /gene="tldD"
                     /locus_tag="ISB17_002148"
     CDS             complement(5122..6567)
                     /gene="tldD"
                     /locus_tag="ISB17_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAP1980081.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPVGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(6723..10523)
                     /locus_tag="ISB17_002149"
     CDS             complement(6723..10523)
                     /locus_tag="ISB17_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAP1980082.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10591..12060)
                     /gene="rng"
                     /locus_tag="ISB17_002150"
     CDS             complement(10591..12060)
                     /gene="rng"
                     /locus_tag="ISB17_002150"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAP1980083.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12050..12643)
                     /gene="yhdE"
                     /locus_tag="ISB17_002151"
     CDS             complement(12050..12643)
                     /gene="yhdE"
                     /locus_tag="ISB17_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAP1980084.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12652..13140)
                     /gene="mreD"
                     /locus_tag="ISB17_002152"
     CDS             complement(12652..13140)
                     /gene="mreD"
                     /locus_tag="ISB17_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP1980085.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13140..14243)
                     /gene="mreC"
                     /locus_tag="ISB17_002153"
     CDS             complement(13140..14243)
                     /gene="mreC"
                     /locus_tag="ISB17_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP1980086.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14309..15352)
                     /locus_tag="ISB17_002154"
     CDS             complement(14309..15352)
                     /locus_tag="ISB17_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAP1980087.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15657..17597)
                     /gene="csrD"
                     /locus_tag="ISB17_002155"
     CDS             complement(15657..17597)
                     /gene="csrD"
                     /locus_tag="ISB17_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAP1980088.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17749..18723
                     /locus_tag="ISB17_002156"
     CDS             17749..18723
                     /locus_tag="ISB17_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1980089.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            19700..20170
                     /gene="accB"
                     /locus_tag="ISB17_002157"
                     /gene_synonym="fabE"
     CDS             19700..20170
                     /gene="accB"
                     /locus_tag="ISB17_002157"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP1980090.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20181..21530
                     /gene="accC"
                     /locus_tag="ISB17_002158"
     CDS             20181..21530
                     /gene="accC"
                     /locus_tag="ISB17_002158"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP1980091.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21622..22668)
                     /locus_tag="ISB17_002159"
     CDS             complement(21622..22668)
                     /locus_tag="ISB17_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP1980092.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22665..23627)
                     /locus_tag="ISB17_002160"
     CDS             complement(22665..23627)
                     /locus_tag="ISB17_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1980093.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(23649..24638)
                     /locus_tag="ISB17_002161"
     CDS             complement(23649..24638)
                     /locus_tag="ISB17_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1980094.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24639..26138)
                     /locus_tag="ISB17_002162"
     CDS             complement(24639..26138)
                     /locus_tag="ISB17_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1980095.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(26199..27089)
                     /locus_tag="ISB17_002163"
     CDS             complement(26199..27089)
                     /locus_tag="ISB17_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1980096.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(27125..27979)
                     /locus_tag="ISB17_002164"
     CDS             complement(27125..27979)
                     /locus_tag="ISB17_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HAP1980097.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         7069 a         7541 c         6687 g         6822 t
ORIGIN      
        1 aattagttca ataattagtg tatacttgat tttgtgatat gggtcacgaa acaaaggccc
       61 agctaaaaga ttatgtcgag gtaaaaatca tgaaaatcaa aaccactgtt gctgcattaa
      121 gcgtactttc tgttctctct ttcggtgcat tcgctgccga ctccattgat gctgcacaag
      181 cacaaaatcg tgaagcaatc gggaccgtat ccgtaagtgg tgtggcgtct tcgccaatgg
      241 atatgcgtga aatgctgaac aaaaaagcgg aagagaaagg cgcaacggcc taccagatta
      301 ctgaagctcg tagtggtgac acctggcacg ctacggctga actgtacaaa taaaccctca
      361 tcgtcttgtc cgacgatatt gcccccggtt cgggggcttt ttttgcgcta atgacgaaca
      421 ttaaaacgca aatgcccttc cagctcttcc tctgcctcat caaacagcaa tattaacgcg
      481 ccaaaacggc gacgcgtttt ctctcccaga tgaacaaatt caatctcaag tggtagcggc
      541 aggacatcgt tcattaacac atcccatagt gagtcgagat cgcgtacctt atctttcgcc
      601 agaccaaagg tttggctaaa gtcacgataa aaatcctcct gactctcaat ctcatcaaaa
      661 tcaaaggtat aaatattcat ctgttgccac cgtcacgttt cgcggcgagt tctgccgttc
      721 atcgctaagt atagccatga aaaaaccgac gcttcaggcg tcggttttga cttaactatc
      781 ggtcaacgca tgttgatact tatggagcat acccgtaagc cgatgtactg gttccgtcac
      841 ctgcggtggc gcttgccaga tgcgtagctt ttcctggtag atttccagtt cttccagcaa
      901 ctggccaaag taccgacgac gtttatcatc gctacgggca gatatcacat ggtctgctgt
      961 gcggcgcatt tgtcggtgaa acgccgataa atcctcgtta accgggatcg gcgcatcacg
     1021 caggcgctgg tgcgcgataa tcatcgtcag cgccaggcga aatttcggca aatcccctgg
     1081 gaacttattc atcagcaaaa acagctgctg ataaagtgcc gggaggtgat tctctttacg
     1141 acgtgccaca ttggtagtca tcgcggaaac agcggcagaa acaaactgat taagcagtac
     1201 acgtccagtc ctgtcgcgcg atttatcccg caccagcaaa ataacggtga acgcgagcac
     1261 acagccgacg atttgcccta atgcgctgtc gagaaactga ctgaaatgga aagtcatcgg
     1321 gttatccagc acgataatgt ttatggtgct ggccaacgcc cccatcgagc ccaatctccg
     1381 tttctgtact tctatgccga ggaaaaatcc cagcactgcc aggctaatac acagcagcaa
     1441 catgctctgt tgggtattag ggataatcac caaaaagtag agcagcccta acggcagcgc
     1501 tgccagcgtc ccgtagataa agtcgatcgc caccatgcat ggattcggca aacgcattgc
     1561 cagtgacgtc acgaccgcaa tcatcaccat tgcaccactg ccggaagtcc agcccgtcca
     1621 cagccagaaa agcgtgccca gaatgcaaga aagtgtggtt cgccagaagt taaccattgc
     1681 atgatgacgt tcggctgact ctacttttac ttccggttcg ccttgcagga tctcttcttc
     1741 ggtggcgttg atttttgtgt tactgataac gccgcgcttg agaagctgat aacgcgttgc
     1801 cgccgcgacc caactataaa tggtaacagg cgtttcccgt tccccggtcc aggcgatcac
     1861 tctccgcagg cgtttgagct gcttgtggac gtcctgcgca gtttctaccg gcgtgtcaaa
     1921 aaattcgcgg aaagtatcag tgatcaattc cgggcgcgta ttctgaataa gataagtttc
     1981 gcaggattgg gtaatcagcg tcagcgatag cgtattgatc gcttttaaac gtcgattagc
     2041 ccgcgcccag cgggaagatt ccatattcag gttgctgcgc ataccttgta acgccgtagt
     2101 acgtcgcacc agatcgcccc aggctttatc gacaacttca ccatcgccat gcttgataca
     2161 gagttgcatt aattgatatt gcgcgaccag caaactttcc agctctcgat ccacttcttg
     2221 tttgatcgat cgcggagaaa agagcaaatc cgccataatt gcacacacaa taccgataac
     2281 gatctcgcta caacgttcga cggcaaactg cggcgtaagc aatggttccg gctgaatggt
     2341 gatcacaatg atcagcgcgg tataaccggc cagcccccac gcatacgagt tttctattcg
     2401 taccagcgag gatatccagg tacaaaaacc ggcccagata cagcacacca gaatcatcaa
     2461 taatggtgct cggatcatcg caatgatgat taccagtccg gcaatacagc caataaatgt
     2521 gccgatgatg cgcaaaaagc cacgatagcg aatagcgcca gaatacggtt cacctcccgc
     2581 agcaaaggcc ggaccggcgg caacaatcgc cgctgtcagt accgcccagc gtggcgtttc
     2641 cagctggaag tgaaagccaa caaacagcgc cagtacaatg gcggtcgcca gttttaccgc
     2701 aaagcgaata tgttgattag caatggagaa aatacccatc gtgattaacc aaactcacgc
     2761 aggcgatgag ccattttacg gaagaacgaa tcctgacttt cgtcgcgatc ttgtttgcca
     2821 gtgactacca ccgtagcagt ggtgcccgca ggccagatgt tctcttgctg gttgtcgaga
     2881 cgaatacgta ccggaacacg ttgcgccaaa cgtacccatt caaggttaga gtcgatggtc
     2941 gccatccctt tgtcgtcacg cgtgctgctg gcattggtga cccctgcggc aacactatca
     3001 acagtccctt tcagcacttt gttactgcca agcggcgtga tctccgcacg atacccaggg
     3061 cgcacccctt ccagcttagt ttcttccata taggccagta catagaagga gttctgtttc
     3121 accagcgcaa ccgccgttga tccacgggta ataaactcac cggtatagac gttgaggttg
     3181 gtcacccagc catctgctgg cgcgcggatc accgtgcgtt caagatccag ttttgccaga
     3241 tcgcgggtcg cctgcgcttt cgctaactga tgcagaaccg tttgtagtac gttgttggcc
     3301 tggtcgatct cttcgcggga catcgcctgc acaccgagac ggttacgacg tccggcctcc
     3361 tggcgtttct cctgtgccag tacctgataa taagcaacat cggcttgcgc ttcctcaagc
     3421 gccttttgat agcgcggctg gtcgatggtg aacagtacct gtcctttttt caccagctgg
     3481 ttatcatgaa cattcacctg ggtaatgagt ccagaaacgt ccggcgcgat cgcaacgacg
     3541 tcagcgctaa agcgcgcgtc acgcgtccac ggggattcgg tgtaatagac ccaggcatta
     3601 aaaattgcga tgaaggccag aatgactaat acgaccgtga tggccgtacg ggagaatttt
     3661 cttattagtg ttttcacttc aacctcaaac gaacagtcgc gatatcaaat aaaacaagca
     3721 gcaatagagc gcggtgttga acaacgccgg atgccagaca aagtcgtaga tacctgttgg
     3781 cacaagtacc cggcgcacca gccagaaaat cgccagtgat aaaagcaatt cgaaaaatat
     3841 cggtgggaag gacagcccaa acaccacgat aacgggaaac agactcatgt tgaccttggt
     3901 tgtatagaga gagcaggcgt tattattttc agcatctgtc gccgcagaga agggcataga
     3961 aagccgggcg agagcaacat tgctgtagat tgatatttaa tatattaacg taactgttat
     4021 gctgttatct atattatgtg atctaaatca cttttaagtc agagtgaata atggaacgac
     4081 taaaacgcat gtcggtgttt gccaaagtag ttgaatttgg ctcttttacc gccgccgcca
     4141 gacagctaca gatgagcgtt tcgtccatca gtcagacggt gtcaaaactg gaagatgagt
     4201 tgcaggtaaa gctgttaaac cgtagcacac gcagcattgg cctgaccgaa gccggtagaa
     4261 tttactacca gggctgccgt cggatgctcc atgaagtgca ggatgttcat gagcaactgt
     4321 atgccttcaa taacaccccc atcgggacgc tgcgcattgg ctgttcttca actatggcac
     4381 aaaatgttct cgccgggctg acagcaaaaa tgctgaaaga atacccaggt ttgagcgtca
     4441 atctggttac cggaattcct gcccccgacc tgattgccga cggtctggat gtggtgatcc
     4501 gcgtcggcgc gttgcaagat tccagcctgt tttcccgccg tctgggcgcg atgccaatgg
     4561 tggtgtgcgc cgcaaaaagc tatctcacac aatacggcat accggaaaaa cccgccgatt
     4621 taagtagtca ctcatggctt gaatacagcg tgcggcccga caatgaattt gaactgatcg
     4681 caccggaagg gatctcgact cgcctgatcc cacaaggaag atttgtgact aatgatccga
     4741 tgacgctggt gcgctggctg acggcgggtg ccgggatcgc ctacgtgccg ctgatgtggg
     4801 tgatcaacga gatcaatcgt ggggagctgg agatcctgct gccacgttac cagtcagatc
     4861 cacgcccggt ttatgcgtta tataccgaaa aagataagct gccgctgaag gtacaggtcg
     4921 tgatcaactc gctgacggat tattttgttg aggtcggtaa attgtttcag gagatgcacg
     4981 ggcgcgggaa agagaagtaa ttaatattgt actgttacgt tgtacaaacc tgtgccgacg
     5041 ggttccccta accccagagg ggcgagggga ccgttcgtgc acggagaagg attaattatc
     5101 cttctgaaaa tactgaaatt attacgcagt accgccaaca gtcaggttat taactttcag
     5161 cgttggctgg cccacgccaa ccggcaaact ttgcccttct ttaccgcaga cacccacgcc
     5221 gttatccagt ttcaggtcgt tgccaaccat cgaaatctgc tgcatggttt cgataccgga
     5281 accaatcaac gttgcgcctt tcaccggctt agttacttta ccgttttcaa tcagatacgc
     5341 ttctgaggtg gagaaaacga atttgccgga ggtgatatcc acctgaccgc caccaaagtt
     5401 cggtgcatag ataccgtact caacggattc aataatttcc tgcggggtcg atttacccgg
     5461 cagcatatag gtgttggtca tacgcggcat gggcagatgg gcgtaggatt cacggcgacc
     5521 gttgccagtc ggcgtcatcc ccatcaaacg cgcgttgagt ttatcctgca tgtagccttt
     5581 cagaatgccg ttctcaatca gcacgttgta ctggcctggc gtaccttcgt catcaatcgc
     5641 caccgaacca cggcgatcga ccatcgtgcc atcatcaacc acggtacaca gttctgaagc
     5701 caccaactcc ccgacctgtc cactaaatac tgaagtgcca cggcggttga agtcgccttc
     5761 cagaccgtga cccaccgctt catgcaacag cacgcccggc caacctgcgc caagtactac
     5821 cggcatggtg cccactggtg cagcaacggc agaaagattg accagcgcca tacgcactgc
     5881 ttcttttgcc cacgcatccg cacggacttc gccgtcgaga tcggcaagga agaactcata
     5941 accaaaacga ccgccgccac cactggcacc gcgttcgcgt ttgccatctt cttcgaccaa
     6001 aacgctcacg gaaagacgca ccagcggacg gacatccgcc gctagtgtgc cgtcggtggc
     6061 cgcaaccaga attaattcat agacaccact gaggctggca gtcacttcct gtacgcgctt
     6121 gtccgcttcg cgggcaacct tatcgacgcg acgcaggata tccaacttct cttcacggct
     6181 catgctttgc agcggatcta ccgaggtata taacgggcta tgctctaccg cgcccagcgt
     6241 ctgtactttg ccatcaccac tatcacggac gatggtgcgc gccgcttgtg cactctgttc
     6301 cagcgccagc aggctgattt ggtcagcata agcaaatccg gttttttcac cactgattgc
     6361 acgcacacca acgccctgat cgatgttgta agaaccatct ttaataatac ggtcttctaa
     6421 aacccaggat tcgtgatagc tcgactgaaa atagagatcg ccataatcaa ggcgacgttc
     6481 ggccagttga ccgaggatcg cgaacaagtc ctgatgtttc aggccgttcg ccgctagcaa
     6541 ttgttcactt accaggttca gactcatcgt ttttgctact cgttagttac tgcagtagag
     6601 gattttttac ggctgccgga tgcggcgtga acgccctgtc cggcctacgg ttatgttctg
     6661 gctcgttggc ctttggcaac gattatcata tgagattggg gcaattacgc gccctcgtca
     6721 aatcattgcg ctttttcttt acgcggttgt cgcaacactt cgttgatttg cggatcgtcc
     6781 agcggacccg aaatgtgata gcgcaaaatg gagactttgc tccacagcgg tcccagcact
     6841 ttactggcgg caaacactgc cgcgccaaca atggggttaa ccgcaaaggc cgcagccaca
     6901 cccaccgtcg cagaaatctc tggtgcgaca accgcttcca tattcaggtc gcgacgtacc
     6961 agatttaccg accctttcat ggcgatgtcc gcttccaggc catccaccag cgtgtcgtcg
     7021 gtgtgcataa cgccgtcttt aatccacgcg gtgctgcgaa tggagtcaaa atagaaccct
     7081 tcgccaaaag tgtctctgaa atcaaaacgc agcttacgca tcagggcatc tacgctcaat
     7141 aagcgcagca attgcccggc atgtccggta ttgatttcgg taatttcgcc tttacccagt
     7201 tgagtatgaa tgatgccatt caacgtcgcc tcatctggct gccagggtgc tttgcgccag
     7261 tgtaaatcgt aatccacatt aaatgacgac tggcgtatgg gcgtcgtgac accaaaaaat
     7321 tctgcggcgg catcaatttt ctgcccgcgc agttttcctt tcagcgaggt tcgttcattc
     7381 cccggattgt taacccattc accatcggca gtgagccgcg caaaaccagt atcaatcagt
     7441 ccattagtca gcgttaacgt atcgccagaa atggtgatat cactgtcaat gcgaccgaat
     7501 ttttgccccc agaaccagca ctctgcgcat cgtatttgtg cgtctggcca gccacggaag
     7561 ctaatgcgtt ccgttgtcgg gaaaggtgat gactgtgttg aatcaccacg agttttcgcc
     7621 acgcttgggt tgtaataaag atatttgata ttcgccagcc acggcgcgtt attacgcatc
     7681 gccagcgtgg cgttgatttc acgcccttgc gcctcaacca gggtgccatt tgccgtcggt
     7741 tgcgaaacaa tactcaggtt attccattgc tgatttccca gcgacaacat aggcgtacgt
     7801 aacgttatgt gttgtgggaa actcgctgca ccaccgacac tctccgccgc gcctttctgg
     7861 aacagggcca gccactcggc accattcatc ggcggcatat tgagttcaac gccactttgt
     7921 tccggcaacg gcgggagcgt tttactgtct gccgcccaaa tagcacgatc gagcgtcagc
     7981 ttttgaccga gcaaccagcg gctattgaaa tagttatccg caccagcctg tccggttaat
     8041 tcaaagctgt tgagattgcc atcaacctta acgtttaccg atagtggttc acccgcaggt
     8101 ttggctaacg gtgaaggtaa gtgactgctc acattcttca aatcgccgtt cagctctacg
     8161 ttatacgtcg cgccagcatg ataaggcagc tcaatgccca ctttaccatc ccacgccacg
     8221 ctgccactta atgcttcgtt caccgctgca ggcagaacgc cggttttcgc tggttgccag
     8281 ttaccgttga gattcaccgc gacctggtag gcttttgccc cttctttggt ggaaaaatcc
     8341 acgttcaacg gctgattaaa ccagcttgct gtcagtggtt cactttgcag atcgccattg
     8401 ataaagctga atttaccgct caaatttttc agggtgctgt cgagtggttt gataaacaga
     8461 ctgttattac gcagcgtcac ttcacctttc gcggttacca actcgccgtt cagcgggata
     8521 tcaagatgta agcgagcatt cacatcgccg tcgagctgga gttcttgcag ggtcgcaccc
     8581 agggaatctt tcagcggtgt ctcatcaaag taagggccaa cggctttacc cggaccttta
     8641 atgtcagcgt caatcagcag tttttctttt gagtagtcag ggatcactgc ggtgagatta
     8701 ctcgcgcgca cgccgcccag attaacgcca tcggttttca tccataaacc gtcgttaata
     8761 aagtccagtt caatatcaag gttagttaat gcaggccagt ccggctggaa ggcaaacttc
     8821 gcgttgcgca gcggcaccag cacttcaaac tgaccttcgt tgtgtttata ggggaagagt
     8881 tgcggattgc caccataaac cagcgtcgcg ttatccgctt caccgccctg aatcgcgcca
     8941 cttaagtaat caaccaggtc tttacccatc aagttttccg ggaaatagcg ccaggcttgt
     9001 gaaccatcat cggtactgat gccagccaga atacccagcc agggttcatc gttagcaggt
     9061 tgcaggtaac gaaaaccgcc gcgcgcatgg acggctttgg ctttaacgtc aatattacgc
     9121 ccatccagct ggaaaccttt gtcattgttc agccagctta tcgttgcctg gccgtcggcg
     9181 atttccagtg gcgcacggaa taccgtttcg taaggcatct ttgcctgctt catcgacgca
     9241 gtaagcaaac cattttctac gctgccggaa agcgtcccgg agaagtgttc cgcacccggt
     9301 aataatttcc attgcttcca ggccagatcg ctccacgatg cctgaaaacg ggtcttgtct
     9361 gccgcctgaa gcgggatatc cagcgccaga gtgttaatct tgccgctcgg ttgtgtggag
     9421 cgccaaacat cacccagtgc aggtgaaagt ttcgcgacca gcgggcgtac gccctccagc
     9481 cctgccagct ccagattact ggcacgaatc cggagttcgt cactgcgttt attgtctttg
     9541 ccgccaacgt cctgttccgg tatccaggcc agcgtcaatg ctccgctcgg ccagggtttg
     9601 ccgtccatcg tgatccgtgt atcgggaata gagaactgcc atcccggatt ttcacgcgta
     9661 atatgcgcag tcagattatc caccgacagc gtatgcgttt ccttctcgcc caaccagctg
     9721 gcaccgccct gtttcagcca gacgtcaccg ccggttacat cgcctttgtc gatcgtcatc
     9781 cagccttcaa gggagaactg tgccgtttcc agcgcaatat tgtcctgcat ccatttaccg
     9841 agccacggct tcaggtcgat gtcatccgcc tggagccaga cgcgaccatt gcttaacaac
     9901 ccctcatcat cgcgcaaatc catacgcacc tgcatcacgc cgtgctgtcc ggtaaggctg
     9961 gagaggctta ccaggccttc cgcacggtgt cgacgtggat cgttcagcca ggtgagctgt
    10021 gggatcgcca gctcggcgcg ctgaccggat ggcgtcagga aactgacttc actgtcgcgg
    10081 agatcgaaat ggtcaaattg acgaagaaac agatcgctga tgtgactggc ttccaggcta
    10141 tcattaccac cgccgctggt gatagaagtg ttggtgcgaa agcgcagctg ccagaaagtg
    10201 aggtcgcgaa actgccagcg catatgtaac aggctctgcc agacatccag cgccagggta
    10261 acgcgtttaa ccgaaaattc gccgccatct tttagttctg cacggatgtc gtgcgcttca
    10321 agcgtcggac caaaattctg ccagctggct gagagctgac tggcttctac cggcatgcca
    10381 gtcgcggatt ctattttgtt gaggatttcc ggacgccagg cgtcaagatg cggtaaagca
    10441 atacgcaggc cgctaaccag cagggcagcg atcacaacga gcgcggctcc agtaagcagt
    10501 aaaatccccg gcaatcgcct cacccgtcac tccttgtctg ctaaaaatgt gactcaaaaa
    10561 ccctttgccg gatggcggcc cagcatctgt ttacatcatt acgacgtcaa actgctcctg
    10621 gttatagagc ggttcaattt gtactttaac ctgtttgcca acgaaaattt ccacttccgc
    10681 cagcgagtgt gactcttcgc ctttcaaggc ttcggctact gccggagaag catagaccag
    10741 gaaacggtcg gagtcgtaag catggtggac acgaacaatc tcgcgcatga tttcatagca
    10801 taccgtttcc acggttttca ccgttccgcg accgtggcag gttgggcatt cgttacacag
    10861 tacgtgctca atgctttcgc gggtgcgttt acgcgtcatc tccaccagcc ccagcgccga
    10921 aaaaccatta acgctggttt tcacccgatc tttgctcaac gcctgctcca gcgagtgcag
    10981 cacgcggcga cggtgatctt cattattcat atcgatgaaa tcaataatga taatcccgcc
    11041 cagattgcgc aaccgtaact ggcgagcgat agcctgcgtc gcttcaatat tggtattgaa
    11101 aatggtgtcg tccagattgc gatgaccgac aaacgccccg gtattgatgt ccacggtggt
    11161 catcgcttcg gtctggtcga taatcagata accaccggat ttcagttcta ctttgcgttc
    11221 cagcgctcgc tggatttcgt tttcgacatc aaagagatcg aaaatcggct ggcgtccggt
    11281 gtaatgctcc agcttgctgg tcatctcggg aatgtactct gaggtaaact ccagcaatgc
    11341 ttcgtaagtc aggcgtgagt caacgcgaat gcggtcgagt tcggcgtcgg cgaaatcacg
    11401 cagaacacgc tgcgccagcg ccagttcgcc gtacagctga taacgggtct gcgggcgttt
    11461 tttacgctcc ataactttgg tccagacgcg tttcagataa gcagcatcgg aggccagttc
    11521 ggcctcgcca accccttccg ctgcagtacg gatgataaac ccgccctgct cgtcgcaata
    11581 ctctgcgacc acttttttca ggcgttcacg ttctgattcg ctttcaattc gttgggatac
    11641 cccaacgtga gaagcccctg gcataaacac cagatagcga gaaggcagcg tgatatcggt
    11701 ggtcaggcgc gcacccttag tgccaagcgg atctttcacc acctgcacca tcagatcttg
    11761 cccctgacgg accagttccg agatgtcgcg caccgtgaat tgcttttgtt cttcacccgc
    11821 cacacattcg gtgtgcggca tgatgtcaga tgcatgaaga aacgcggctt tatccagccc
    11881 aatatctaca aaagccgcct gcatacccgg aagtacacga cttacacgac ccttgtagat
    11941 attgcctact attccgcgtc gcgcctcacg ttcaatatga atttcctgca gaataccgcc
    12001 atcaatatac gccactcgag tttccgaagg cgttacgttt accaacaatt cagccgtcat
    12061 gtttatccct tttctcacgc agtgcgttaa aattacttaa taattcatac gtttcaacca
    12121 gcggtaagcc gactacggcg tgaaagctgc catttatctt cctgacaaaa cagccaccca
    12181 gcccctgaat accgtatgca cctgctttat ctaacggttc gccgctggcg acatagcccg
    12241 cgatgtcttc gtctgttaac gttctgaaag tcacatcggt gaccaccagg caatcgagaa
    12301 tgtgctggct gtcggccaac gccactgccg tcatcacctg atgggtctga cccgataatt
    12361 tgcgcaacat ctgcgccgca tgctctgcgt cgcgcggttt ctcgagcact tctccattca
    12421 ggataacgat agtatccgca cccagcaccg ggagatcctg cgccgtttgc gcgacacccg
    12481 cctgtgcttt ctcgcgcgcc agacgcacaa catactgctg cgcgctctcc tgcggctgac
    12541 gctgctcctc aatgcccgta acaatacgtt caaaggtcac gccaagttgc gcaagtaact
    12601 cctgacgacg cggagaaccg gaagctaaat acagagaagt catagaaacc tttattgcac
    12661 tgcaaactgc tgacggactt tacgcatcag caagaaaatc cacggccaga gaacgccatt
    12721 gactacacta ctccagaaca cttccggtct gaaagagacg ttaatcacta aaaactctgc
    12781 ccagaaaaca ataatatcca ccaccagcga aagcaacatg acgaccagcg cctgctgcca
    12841 taatgcgagg ttgcggaaaa gctggtattt cagcgccacc aggtaagcaa tgatgctcat
    12901 cgccaatacg cgtacgccaa gcgtcgagcc gctgatcaga tccagtatgg cacccatcac
    12961 aaaacctgtg cccacattta cgcgatgggg caaggccagg atccaataca acaagatgag
    13021 taacacccag tttggccgga aaacaatcag gttgtccggc catggcatga tttgcagcaa
    13081 cagcgcaatg aggaaagaga gccagattac ccagcgtccc tggctacgat agctcgccac
    13141 tattgtcctc ccggcgcacg cgcaggcggt tgagcaccac tttgcggcgg cgtagccctt
    13201 tgtggagagc gattagcagc gggctgtgtg ggcgcagcag gcgcagtaac tgcatttcct
    13261 gtcgccggtt gctgcggagt cggctgagtg atccccgttg ccggttcagg taactttggc
    13321 cccatcgcgt ctggcgaagg caatacctgc ggcatcatct gcatcagacg ttcattagca
    13381 acacgatgca cctcttccgg cgtcatcggg ttagcgccgt tacgatccgc gccccacagc
    13441 agcagcagat aacgcaaacg ttgcagtcct gcagtcggac gcgcctgaat cacagtataa
    13501 gcgcgctggg tatcgagttt tacggaagag acaaccgcga ccggatagcc ttccgggaaa
    13561 cgaccgccca gaccggaagt caccagcaca tcaccaacac gaatatccgt attcgctggc
    13621 agatgctcaa gctgcaaatc atccgtacaa ccgttaccgg ctgcaattac gcggatatcg
    13681 ttgcgcagca cctggattgg cagcgcgtgg gtcgcatcac aaatcagcag cacgcggctg
    13741 gtcagtttag cgacggccac cacctgacca acaacacctt tgtcgctgat aaccggctgg
    13801 ccttcataaa cgccattaac gctaccttta tcgataacaa cttgatcgct ataaggatcg
    13861 ttaaccgtgg agataacctg agtcaccatt ttctgctcat cctgacgcag cggggaaccc
    13921 agcagctcgc gcagacgcgc gttctcctgt ttgtattgtc caagcatcag cagttcactg
    13981 tttttcagca acagttcctg acgtaacgcc cggttttcaa gttctagttg gtcacgcgag
    14041 gccagcgtct gcgatacgcc atccagcaat tcacgaggag cattggaaac aaagtagaaa
    14101 ggactgacgg cggtatccat ataagtacgg atttgactga acgtccccag gcggctgtcg
    14161 gcaataataa tgccgagcgc caccagcacc gccagaataa ggcgaatctg tagcgacggg
    14221 ccacggctaa aaattggctt cataagttat gcgtattctc gtatcagacc aggcagggta
    14281 aacagacact tcccctgcct gcatccgatt actcttcgct gaacaggtcg ccgccgtgca
    14341 tgtcgatcat ttccagcgct ttgccgccac cgcgcgccac acaggtcagc gggtcttcag
    14401 caacaacgac tggaatgccg gtttcttcca ttaacaaacg gtcaaggtta cgcagcagtg
    14461 cgccaccacc ggtgagcacc atgccgcgct cggagatgtc ggaagccagt tccggcgggc
    14521 actgttccag tgcaaccatt accgcgctca caataccggt cagcggttcc tgcagtgctt
    14581 cgaggatttc gttggagttc agggtaaaac cgcgtggaac accttctgca aggttacggc
    14641 cacgaacttc gatttcacgg acttcatcgc ccgggtaagc cgaaccgatt tcgtgcttga
    14701 tacgttctgc ggtggcttca ccgatcagag aaccgtaatt acgacgtaca tagttgatga
    14761 tagcttcgtc gaaacggtca ccaccaatgc gcacggaaga ggagtaaacc acgccgttca
    14821 aggagataac agcaacttca gtagtaccac cgccgatatc aaccaccata gaaccggtcg
    14881 cttcagaaac cggcaggcca gcaccaattg cggcagccat cggttcttca atcaggaaga
    14941 cttcacgggc accagcgccc tgcgcggatt cacgaattgc gcggcgttca acctgggtcg
    15001 cgccaaccgg cacacaaacc agaacgcgcg ggcttggacg cataaagctg ttgctgtgca
    15061 cttgtttgat gaagtgctgg agcatttttt cagtcacgaa gaagtcggcg ataacgccgt
    15121 ctttcattgg gcgaatggca gcaatattgc ccggcgtacg gcccagcatc tgcttcgcgt
    15181 catgacctac tgcagctacg cttttcggtg aaccggcacg atcctgacga atggccacca
    15241 cggaaggctc attcaatacg atgccttgtc cttttacata aatgagggta ttcgcagtac
    15301 ccaggtcaat ggacaagtca ttggaaaaca tgccacgaaa ttttttcaac atactaaggg
    15361 ataatcctga aagctggggc ggaaaagaaa atccgcttac tttaccaacc acacgcagca
    15421 gcgacaaggc gcaaaaatca tctgctacgg tgaaaattag tgcagttcgt ttcctttgtt
    15481 acaaatctct gcctgagtcc agaaaggctt aatgcatcag cagcattcct cgcctgtttg
    15541 caaccgcgta aggtcattca tctgcatatg tgctgcaaca atctggcgag cagacaagca
    15601 cactcccatg agacgcagcg cgcgttattc tacgtgaaaa cggattaaac ggcaggttaa
    15661 accgagtatc tttgtgaata ttttttcacg ttagtatcaa gtggctgtga ggacgcgaaa
    15721 aaatcccctt gcccacctgt aacaccgcgc tgaatcaggg tctgccactc gcttcgcgaa
    15781 cgcacgccgg tggcgtaaac ctgggtgctg gtcccggagc aggcttccac caggctttga
    15841 accagcagct ggttctccgt tcgcttctca atgtttctga ccagccccgg atggagcttg
    15901 agtaactcaa cattaagttc tttgatccaa ctggtgctta ccagcgtcaa accagcctgg
    15961 ttgacggcta cccgtacccc taaagcattc actaaacgaa taacaggttg taagcgactg
    16021 atatgttgac caacatcggc ctctgcaagt tcaataatta tgcgtcttcg ttgcgatttt
    16081 tcacattgca ttaacgtatc gcgcagccaa cgctgaaaac gcgggcgaat cagcgactca
    16141 acggtaacct gaatcgccag attttcctct ggccagtaac gcaatagtgg aataagacgg
    16201 ctgattagca agcggtcata ctcttccgat aaaccaaact gcaagaccat cggcatatac
    16261 tctgccgagc taacctcttc attaccatcg aagatgcggc acatgagttc acgatgatga
    16321 acccggcctt cgcgagtaac cgccggtttt tgataaaggc gcgggccgcc gcgactgagc
    16381 atttgctcga taagcgtacg ccagcgaaca ttaccgcgtc ctttttcagg caacgagtca
    16441 tcgtaaatag cccagctatt gccgccctgc aatcccgcat tacgcgtggc agactctgca
    16501 tgttccatta cctgctcggt atcctgacca ctacgccagg cacagatacc aatgtggatc
    16561 atatcgtcgc gatcgagcat tttattgttc ggcaaggtat cgacggcttt gattaactga
    16621 ctggcgatgc tctctgcctc ttttagcgtc cggtgcggta acagcgcagc aaaatcactg
    16681 cgatggtaac gcgccagcag tgcgccaggg tagcgcatca taaatgtcga tagcagattc
    16741 gtcagagaga agaattgttc ttcaaccagg ctgtgccccc aggtatcact caacatattg
    16801 aaatccggca gacgaatcat catcacgatc ccgtgggtac ctactttctc ttgatcttcc
    16861 agtaacgttg ctaactgatt atcgaaaaag agtcggttat tgaggccggt tttcatgtcc
    16921 tgggcggcat aagagcggat cagcgtatca agacggctgt gttgttcgcg tgcgttctga
    16981 atttcacaaa gcagcgtatc cagcgcactg ctggttctgg gcggccattc atagatggtt
    17041 cccaacacat tagagccacg ctcaccgttt aagatacgag tagcccgggt ttccagcaat
    17101 tcttgcccgg caagttgccg ttgtaaccag cgtaccgcca ggaagagcat aagaatgata
    17161 aagccaatcg cccccgtgag cggcgcggtg gtcattaaag aatggaaata gttgcccatc
    17221 ggatcctgat aaaccagacg cagcgacatc cccggatgct ttatcaacgg aacgctcagt
    17281 tcacgaaaca gatcgttggt gccaaccgga cgataactac catttctggc cagggtataa
    17341 acctgcttat caccatggag caggtctaca cgaacgatat ctgccgacat cattaattcg
    17401 gtaatttgtg gccttaatgt gctgaagtca ttcgacagta ggtgggtatc aatcgccgtc
    17461 gccaccgcct gaacacgatg actaaactta tactgaatgg cgttgtagaa acttagcgaa
    17521 cagcccagca aagtcacaaa aattgttaac ccggtgagca gcgtgacaaa ggccgaaaat
    17581 ttcgtcgtta atctcatcct tgtgttaact ccgatagtga ggaagcgggc atactagcaa
    17641 atcagattta tctcgcaatt tattgcgctt catcggcttt gcttttccat tagcgagtat
    17701 agtcttcaga aattattttc caatccatca tgcacatgag gaccacttat gcaggcgtta
    17761 cttttagaac agcaagacgg caaaactctc gcatcagtac agactctgga cgaaagtcgc
    17821 ctgccggaag gcgatgtcac ggttgatgtt cactggtcga gcctgaacta caaagatgcg
    17881 ctggcgatta ccggtaaggg aaaaatcatc cgtaattttc cgatgattcc tgggatcgat
    17941 tttgccggaa ctgtacgcac cagcgaagat ccgcgttttc atgccggtca ggaggtgtta
    18001 ctcactggct ggggcgttgg tgaaaaccac tggggtgggc tggcggaaca ggcgcgagtg
    18061 aaaggtgact ggctggttgc catgccgcaa gggctggacg cgcgtaaagc aatgattatc
    18121 ggtactgccg gttttaccgc catgctgtgt gtgatggcgc tggaagatgc cggtgttcgc
    18181 ccgcaggatg gggagattgt cgtgacgggt gccagtggtg gcgtcggcag taccgccgtg
    18241 gcgctgctgc ataagttggg ttatcaggtc gttgctgttt ccggtcgcga aagtacccat
    18301 gaatatctga aaagtttagg tgccagccgt attcttcctc gcgatgagtt tgccgaatcc
    18361 cgtcctctgg aaaaacaagt ctgggctggg gcaattgaca ccgttggcga caaagtgctg
    18421 gcaaaagtgc tggcgcaaat gaattacggc ggctgcgtgg cggcctgtgg tctggcgggt
    18481 ggttttactc tgccaaccac ggtcatgcca tttattctgc gtaatgtccg tttgcaaggg
    18541 gtggattcag taatgacgcc accagcacgc cgtgcacaag cctggcagcg actggtcacc
    18601 gatttaccgg aatcattcta tacccaggcg gcaaaagaga tatctctggc agaggcaccg
    18661 aagtttgccg aggccatcat taataaccag atccagggtc gcacgctggt gaaggttaac
    18721 taaccattta gcagggaata ataagagagg gaactcattt tgaaatcatt attcaatcag
    18781 ttaacgggaa aagcggttag ccggacagct ttcgtcgaac atcttggtta tgaagttgta
    18841 caacatcatc caaactggaa agtcatgatt tcgactgacc ataaattgat gcgcattgat
    18901 gctccaccaa acagccatta ttgatacgcc tccgtcgcct gttaggttta tggtgctttg
    18961 ccttggcgac gctacgctta atctgttact gatttctggt accatggggg gaataatctg
    19021 attttatttg attacaaatt aaccacttaa acctattgaa tgcggagcat tgtcagtcgg
    19081 ttaattttgc gtgctttagc attcacatct ttccggacga tgcagtgaaa attgggtaat
    19141 ccccagcaac cgctgcgtaa tgtcgtctat cttgtcgcga tcctggcatc cctacattat
    19201 ttgtggtctg tgaagattat ctcattgcag cccccatctt cgcagggctg gctttacagc
    19261 gtttcacctt acgttataag aagttccgtc gatgatggcg ctaatttcgt gaattgtgcg
    19321 gcttgttgca aattacacgg tgttgaaggt tatttacatg ttagctgttg attatcttcc
    19381 ctgataagac cagtatttag ctgccaattg ctacgaaatc gttataatgt gcgacctcgt
    19441 cctccctgac gcagtttttg cactgcggaa aaggtgacat tggcgcaacg aaggtatatt
    19501 ttgttttttg ccggaggata gcagcagatc gctgcacaat gtccgtcaag tctaacattg
    19561 acactctggg gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc tatgcataca
    19621 gctgtcgggg catacgcttt acagacggcg gtgaaacgcc tgtcacaatc acactaaaca
    19681 aagagtacgg aacccactca tggatattcg taagattaaa aaactgatcg agctggttga
    19741 agaatcaggc atctccgaac tggaaatttc tgaaggtgaa gagtcagtac gcattagccg
    19801 tgcagctcct gccgcaagtt tccctgtgat gcaacaagct tacgctgcac ctatgatgca
    19861 gcagccagct caatctaacg cagccgctcc ggcgaccgtt ccttccatgg aagcgccagc
    19921 agcagcggaa atcagtggtc acatcgtacg ttccccgatg gttggtactt tctaccgcac
    19981 cccaagcccg gacgcaaaag cgttcatcga agtgggtcag aaagtaaacg tgggcgatac
    20041 cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc gaagcggaca aatccggtac
    20101 cgtgaaagca attctggtcg aaagtggaca accggtagaa tttgacgagc cgctggtcgt
    20161 catcgagtaa cgaggcgaac atgctggata aaattgttat tgccaaccgc ggcgagattg
    20221 cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct gtgcactcca
    20281 gtgcggatcg cgatctaaaa cacgtattac tggcagatga aacggtctgt atcggccctg
    20341 ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat cagcgccgct gaaatcaccg
    20401 gcgcagtagc aatccatccg ggttacggct tcctctccga gaacgccaat tttgccgagc
    20461 aggttgaacg ctccggcttt atcttcatcg gcccgaaagc agaaaccatt cgcctgatgg
    20521 gcgacaaagt atccgcaatc gcggcgatga aaaaagcagg cgttccttgc gtaccgggtt
    20581 ctgacggccc gctgggcgac gatatggata aaaaccgcgc cattgctaaa cgcattggtt
    20641 atccggtgat tatcaaagcc tccggcggcg gcggtggtcg cggtatgcgc gtagtgcgca
    20701 gcgacgctga actggcacaa tccatctcca tgacccgtgc ggaagcgaaa gcagctttca
    20761 gcaacgatat ggtttacatg gagaaatacc tggaaaatcc tcgccacgtc gagattcagg
    20821 tactggctga cggtcagggc aacgctatct atctggcgga acgtgactgc tccatgcagc
    20881 gccgccacca gaaagtggtc gaagaagcac cggctccggg cattaccccg gaactgcgtc
    20941 gctacatcgg cgaacgttgc gctaaagcgt gtgttgatat cggctatcgc ggtgcaggta
    21001 ctttcgagtt cctgttcgaa aacggcgagt tctatttcat cgaaatgaac acccgtattc
    21061 aggtagaaca cccggttact gaaatgatca ccggcgttga cctgatcaaa gaacagctgc
    21121 gtatcgctgc cggtcaaccg ctgtcgatca agcaagaaga agttcacgtt cgcggccatg
    21181 cggtggaatg ccgtatcaac gccgaagatc cgaacacctt cctgccaagt ccgggcaaaa
    21241 tcacccgttt ccacgcacct ggtggttttg gcgtacgttg ggaatctcat atctacgcgg
    21301 gctacaccgt accgccgtac tatgactcaa tgatcggtaa gctgatttgc tacggtgaaa
    21361 accgtgacgt ggcgattgct cgcatgaaga atgcgctgca ggagctgatc atcgacggta
    21421 tcaaaaccaa cgttgatctg cagatccgca tcatgaatga cgagaacttc cagcatggtg
    21481 gcactaacat ccactatctg gagaaaaaac tcggtcttca ggaaaaataa tttgtgacgc
    21541 gcggtgtttt catcgcgctt agcaaagaac gtaatcaacg ccgggcctgt taatgctacc
    21601 cggcgttttt attgccgtta actaacggta gcctgcaatt tccgctttca acgcgctaat
    21661 ttcagcatcg ccatcaacaa ttcccagctc aaagcagtat ttctttgttt cccctggctc
    21721 aataaagggc agacgcccct gcgctcgttc tttatcacga ccgtcaggaa aactattact
    21781 gacctccagt cccataacat attcacccgc gcctaaattt ttccactgga taaagttggg
    21841 tagctgcatg gtgtcgaatt tctcgtaaat tccccacccc agttcggcgt tcaccagcgc
    21901 aactttactc atcccctgac tgtcgctatt caacgtgaga tagtaaacct gctcgtcaaa
    21961 accgggctgt ggggtacaaa tctccagatg actggttaac ccttccgccg catgcggagt
    22021 tcgtggcgtt atgcttttag ccgacagcaa gatttctgcc tgtggcgata ataaaggcca
    22081 gccgtaatta aagtgataaa gaatttgcag cggctggcgg gtgaaaccat gattagtcac
    22141 cttgtcttca atgcgcatta cattttcgcc ataacggcag cgaatggtcc tttccagcgt
    22201 cagattttca ccgaacaatc gtgtttcccg tactttgcca ttgatgacaa attcaatgct
    22261 gtcgtcggtc cgttctgtgc gataccccac ctcttctgct ggagtcgcag ccaaacgccc
    22321 gtgtaacccc agcgtttctc cttcatcctc acagggtgtg ccaatgtagc taagtccgca
    22381 ggtagtcagt aaccccgcgt aaaagctacg taaaaacccg ttgccctgag actcaaaaaa
    22441 agcgggggcg acaacaccgt tgggggtgat aaaagagagc gcagtgtcct tgtagctggc
    22501 ccatgcaata tccagcgcgc gatcttttac caccgtaaat gccagtcccg caccagtacg
    22561 gacatcaaac gcttctgtac cctttgctct gccactacgt aactcatagt gtttaattcc
    22621 ggcaacctgc gagagatcgc caatccacga ccaaatttcg tttttcattg tgctgcttcc
    22681 cgaatggaca tgtcatggcg ctggatgaac gtttcgattt ccataaggcg agacgttccc
    22741 tccattgggc cacgtcgggt taccgccagt gcaccgcagg cgttggcata ttgtaatgcc
    22801 cgatgggcgt caaaccctag ctggcggcag gcaatccacg cgccaccaaa gcagtctcct
    22861 gcaccggtcg ggtcaacttc ttccaccgga tagctctcaa catgaaactg ctcattcgcg
    22921 gagtagtaac tggctccttg attgccgcgc ttcacaatca cctctttaac accttcttca
    22981 agaaaaccag cgattgcacg ctccggcgtc gagtgtggcg acaagagcaa cacttccccc
    23041 tcactgggca tatagatatc cgttaactca agaacaaagt ggagggcatc gcgcatttct
    23101 ggaatatcaa gcatttcttt acgaatattc gggtcgaaag aaataacgcc accatttgct
    23161 ttaacgatgg ttactgcttt ttttactgcg tctaccatat ggaatgaaaa cagggatgaa
    23221 cccataatat gaaaatgggt acaatccttc agaatatttt catcaacatg ctgtgccgat
    23281 aatttaccgc aagcggcatt cttgatatta aaaataaaat cgcgatcgcc agagttatga
    23341 taagtaacaa aggcactacc ggtggcttcc agaggtaaga ctgaaatccc cctgatgtcc
    23401 acaccgtcag cagccagacg atgtatatta atatcaccaa agccatcatt ccccacgcag
    23461 ctaatgatgc cgcacggaac gccaagacgt gtcacctggt cgataaaaat agcgggcgca
    23521 ccactgggat aagggccgtt ccagatacct ggttgatcaa atggctggcc gattttactc
    23581 gccataattt caaccagaat ttctccaatt gtgaagacct tactcatatg attaattcct
    23641 tgaaatgatt agctgcgttt tcttttattt cttacgtcaa aataaacggc tacaagaatg
    23701 actaatcctt taaccaccat ctggtaaaaa taaggaacat tcattaagtt catgccatta
    23761 ttaataacgc cgatggttaa tgcgccgatc accgtcccca ccacggttcc atagccgccg
    23821 ttcaggctgg tgcctcccag tacagctgcg gcaatagcat ccagttcgta gctcaacgcg
    23881 gaattcggtt gcccggaata gaggcgtgag gcaaggatca tcccgctgat acctgccagc
    23941 acggctgtaa tcataaagac tttaattttc aagcgggtga catttatccc tgaataaatt
    24001 gccgcttctt tattaccacc agtgatataa atttttcgtc caaaggttgt tttagtaaga
    24061 atgaagtggt ttatcagcaa taagatgatt aatatccata taggaatagg tatatagaga
    24121 aattctccat tccccagcgc cagaaaggca tcatctttaa tcatgatggg cataccatcg
    24181 gtcactatat aagcgatacc acggaagatc cccatagtag caacggtgac aataaacgaa
    24241 gggatccccg ctatagccgt caagccacca tttatcatcc ccagagccag tgctgcaata
    24301 atggataatg ggaccaataa aacaaatgga attccccatg aagccccaag cgcagcaaat
    24361 gtgccaatca atgcaatggt tgaaccgacc gataaatcaa tatcgccaag caaaagcaca
    24421 taagtcattc cataggcggt aattgcaatt atcgaaacct gaagaataat attagtaatg
    24481 ttattggcgg taaaaaaact gtcattaata aaactgaaca gaatgattaa caccaccatt
    24541 ccactgagga taccaccata ttgcctgaag acttttttaa aattatttgc aatgggaatt
    24601 cccgaaggtt tcggactggc aagagttttc tctgtcattt aacgtactcc tgttgcagcg
    24661 gtcattacct gtgtctgcgt taaattttca catgaataaa tatcggcaat agttttatta
    24721 cgcattacca ccagacgatc gctaagtgcc atgacttctg gcagttcaga ggacaccaga
    24781 ataattgctg tccctgccgc agccagttga cgtatcacgt tatagatttc aaattttgcg
    24841 ccgacatcca ctccgcgcgt tggctcatcg agcaataaaa tacgcggagt tttctccagc
    24901 cacttcgata acacgatttt ctgttgatta ccgccgctca gtgctcccac tgcctgatgc
    24961 acagaactca ctttagtgtt cattctggca attgattgca gcgtcttcgc ctcttcttgt
    25021 cggtgattga tgagtcccat ccgcgaagcg ttatattgaa acgccacatg gtgcgtgttc
    25081 atattgacgt cgtgcatcag cactaaccct tcttccttgc ggttttcggt cacaaaagca
    25141 atgccatgat caatagcctg tttgggatta gcgatcctga cttccttacc atcaatcaac
    25201 acagtcccgt gatacgaaac gaggccaaat agcgccttca tcacatcgga acgcccggcc
    25261 cccaccaggc caaaaaatcc gaggacttcc cccggacgca cagcgaaact gacctcttta
    25321 aaaagcgaag gatgggaaag attttttact tccagttttg caggaacatt ttcatccggt
    25381 ttttccccaa gacgtgctgg ccaaatattt ttcatttccc gaccaaccat ttgggcaatc
    25441 aagtgctgga tatcactgtt tttcttttca tgcgtagcga tatactcacc gtcgcggatc
    25501 accgaaatat catctgaaat ccgcataatt tcatccatgc gatgagagat ataaataacc
    25561 gcacagcctc gctgctttaa tttttcaata atgttaaaca ggatctccgc ttcattatca
    25621 cttagcgaag atgtcggttc atccataatc acgacatccg ctgcatagct taaggcttta
    25681 gcaatttcta ccagttgcct atgtgccatc gacatattgc cgaccatttc cagagggtct
    25741 agtgggatgc ccagttccgc gaggaggttc tggcaatctg cgagcatttt tttatcgtta
    25801 atgatgccaa aacgtcgcgg ttcattattg gcataaatat tttccgccac ggtcatatta
    25861 ttactcaggc tcagttcctg aaaaataatc gcaatcccta actggcgaga atgctgcgga
    25921 ttattaatgg tgatggtttc accacgtagt gttatcgttc cctgggttgg cggataaaca
    25981 ccggccagta ttttcattaa tgtcgattta ccggcaccat tctcgcctaa taaagtatgc
    26041 acccgccctt tacgtaaaga gagtgtaata tcttttaatg cctgaacatg accaaatgtc
    26101 ttcgcaatat gcgaaacatc aagaataaca tccgtcataa tgacctcggt gtcacaacaa
    26161 aacgccaata acgttttgtt gtgaggtgaa gatatttact atttacctgt ataaacgccg
    26221 ggttgtaccg ggatcatttt ttctaccggt ttaccattga ggtgatcaac agcggcatca
    26281 atggctttag cacccatttg atctggatat tgccgaatca cggccgcaaa cgttttttca
    26341 ctgtcaacgg catcacgcgc ttctttcatg ccgtcaaaac caatgatttt gatgttgtcg
    26401 ttatgcgcag attttgctgc gataaccgct gccagcgccg catcatcacc aaagccaaac
    26461 agaccattca ggttagcatt tgcctgcata atgttttgcg cagtcgttaa cgcttcagct
    26521 cgcgtaatac caggctgttc agcaacaatt ttaatatccg gtgtatcgct taaccctttc
    26581 ttaaaaccat caacgcgcgc aaccaccgat tgtagtgccg gataagaaat aatggctaca
    26641 gtacctttac cactcaaaag tttacccatt aacttgcctg cttcaacgcc tcccgcatag
    26701 ttatcggtcg caacatgcga tacgaccgga acgccttccg cagcgacgtc aacggtgata
    26761 acgggaatac cggccttatc ggctttaatt atcgctgcct ggacgccttt cgaatcgacc
    26821 ggagagataa taatggcatc cactttttta gtaataaaat cctcaacatc agaaagttgt
    26881 ttgtttaaat cctgattggc aatagagaca cttaaattga cattgtcttt ttttgcctct
    26941 tctttcatgg cattcgcaag atcaatatag aaaggatgtt gttgtgttaa taatgacgca
    27001 ccaattttat actcttttgc caacgaactt tgcgagaaca atgccaggct caccaacata
    27061 gctgaaccaa gtaatttaat atttttcata aacaatcctc attatttttg tagggtacaa
    27121 cgtcttaaat ttttccttca ctaccacaaa cgccaataat acgagttacg acttctttca
    27181 tggcggcctt tgcgggctgc atatagtgac gtggatcatt tgccgcagga ttgttgtaga
    27241 aatatccttt taacgcatcg gcaaacgcaa ttttcaattc tgtcgcgaca ttcactttac
    27301 aaataccgcg acgaatacag tgctgaacgt cctgttccga taagcccgaa gcaccatgca
    27361 acaccagcgg aatctccact cgctgacgaa tctgttccag ccgcacaaaa tccagtcgtg
    27421 gttcatgcgc atataaacca tgtgctgtgc cgattgctac cgccagggag tcaatttggg
    27481 tacgttgaac aaactctgta gctgcggctg gcgaggtata agcggaatct ttttcatcca
    27541 cccgcagatc gtcctcctgg ccgccaagac gccctagctc ggcctcaacg ctaacatcaa
    27601 agcgatggca aaaggtggta acttcttgca ctcgcgcaat gttgtcttca aatggcaaat
    27661 gggagccatc aatcatgaca gaacggatcc ctgcgcgaac cttgttaaaa atgtcatcac
    27721 tctcttcatg atggtcgaga tgcaggacta acggcaggtt gtactcgcga gccagcgatt
    27781 gtactaacga aattaaactt cccacgccac cataacgata tgtaccgggt gttcccgcca
    27841 gcataacggg agagcgaagt tgtgcagcgc tctcaacaac tacctgtaaa gtttcaaggt
    27901 tatgaatgtt gaaagcaggg acggcatatc cctctgactg ggcttttttc agcatgtttt
    27961 tgcttgaaat aatgtacatc gcagctcctg ttatttgtcg ttcgccttac aaaatgaaga
    28021 aagagaatct tcactttcat tttgaaagca aactagcaca gcaaaagcag caaaaagcgt
    28081 gatgcatgtc gaacatcatc aaaataactt acaaaaaat
//
LOCUS       DACADA010000052        28061 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.053, whole genome shotgun sequence.
ACCESSION   DACADA010000052 DACADA010000000
VERSION     DACADA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.053"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            175..945
                     /locus_tag="ISB17_002165"
     CDS             175..945
                     /locus_tag="ISB17_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAP1980098.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            960..1901
                     /locus_tag="ISB17_002166"
     CDS             960..1901
                     /locus_tag="ISB17_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP1980099.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            1952..3238
                     /locus_tag="ISB17_002167"
     CDS             1952..3238
                     /locus_tag="ISB17_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1980100.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3235..3522
                     /gene="fixX"
                     /locus_tag="ISB17_002168"
     CDS             3235..3522
                     /gene="fixX"
                     /locus_tag="ISB17_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAP1980101.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKRDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3581..4912
                     /locus_tag="ISB17_002169"
     CDS             3581..4912
                     /locus_tag="ISB17_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980102.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWTLPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            5020..5550
                     /gene="kefF"
                     /locus_tag="ISB17_002170"
     CDS             5020..5550
                     /gene="kefF"
                     /locus_tag="ISB17_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAP1980103.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5543..7405
                     /gene="kefC"
                     /locus_tag="ISB17_002171"
     CDS             5543..7405
                     /gene="kefC"
                     /locus_tag="ISB17_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAP1980104.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRRAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7597..8076
                     /gene="folA"
                     /locus_tag="ISB17_002172"
     CDS             7597..8076
                     /gene="folA"
                     /locus_tag="ISB17_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAP1980105.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8162..8395
                     /locus_tag="ISB17_002173"
     CDS             8162..8395
                     /locus_tag="ISB17_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP1980106.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8398..8682
                     /locus_tag="ISB17_002174"
     CDS             8398..8682
                     /locus_tag="ISB17_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="HAP1980107.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIK"
     gene            complement(8709..9557)
                     /gene="apaH"
                     /locus_tag="ISB17_002175"
     CDS             complement(8709..9557)
                     /gene="apaH"
                     /locus_tag="ISB17_002175"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAP1980108.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(9564..9941)
                     /gene="apaG"
                     /locus_tag="ISB17_002176"
     CDS             complement(9564..9941)
                     /gene="apaG"
                     /locus_tag="ISB17_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAP1980109.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(9944..10765)
                     /gene="rsmA"
                     /locus_tag="ISB17_002177"
     CDS             complement(9944..10765)
                     /gene="rsmA"
                     /locus_tag="ISB17_002177"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP1980110.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVGVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10762..11751)
                     /gene="pdxA"
                     /locus_tag="ISB17_002178"
     CDS             complement(10762..11751)
                     /gene="pdxA"
                     /locus_tag="ISB17_002178"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP1980111.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11751..13037)
                     /gene="surA"
                     /locus_tag="ISB17_002179"
     CDS             complement(11751..13037)
                     /gene="surA"
                     /locus_tag="ISB17_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAP1980112.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(13090..15444)
                     /gene="lptD"
                     /locus_tag="ISB17_002180"
     CDS             complement(13090..15444)
                     /gene="lptD"
                     /locus_tag="ISB17_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAP1980113.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDSPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15699..16514
                     /gene="djlA"
                     /locus_tag="ISB17_002181"
     CDS             15699..16514
                     /gene="djlA"
                     /locus_tag="ISB17_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAP1980114.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGSGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(16664..17323)
                     /gene="rluA"
                     /locus_tag="ISB17_002182"
     CDS             complement(16664..17323)
                     /gene="rluA"
                     /locus_tag="ISB17_002182"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAP1980115.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(17335..20241)
                     /gene="rapA"
                     /locus_tag="ISB17_002183"
     CDS             complement(17335..20241)
                     /gene="rapA"
                     /locus_tag="ISB17_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAP1980116.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(20406..22757)
                     /gene="polB"
                     /locus_tag="ISB17_002184"
     CDS             complement(20406..22757)
                     /gene="polB"
                     /locus_tag="ISB17_002184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAP1980117.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(22832..23527)
                     /gene="araD"
                     /locus_tag="ISB17_002185"
     CDS             complement(22832..23527)
                     /gene="araD"
                     /locus_tag="ISB17_002185"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1980118.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(23642..25144)
                     /gene="araA"
                     /locus_tag="ISB17_002186"
     CDS             complement(23642..25144)
                     /gene="araA"
                     /locus_tag="ISB17_002186"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP1980119.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(25155..26855)
                     /gene="araB"
                     /locus_tag="ISB17_002187"
     CDS             complement(25155..26855)
                     /gene="araB"
                     /locus_tag="ISB17_002187"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAP1980120.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELCVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRNIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEVWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            27194..28039
                     /gene="araC"
                     /locus_tag="ISB17_002188"
     CDS             27194..28039
                     /gene="araC"
                     /locus_tag="ISB17_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAP1980121.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWVNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         6255 a         7354 c         7755 g         6697 t
ORIGIN      
        1 acgccatgtt ttcaatattg gtgaggagct taacaatatt gaaagttgga tttatctgcg
       61 tgtgacattt tcaatattgg tgattaaagt tttatttcca aattaaaggg cgtgatatct
      121 gtaattaaca ccaccgatat gaacgacgtt tccttcatga tttctggaga tgcaatgaag
      181 attattactt gctataagtg cgtgcctgat gaacaggata ttgcggtcaa taatgctgat
      241 ggttcgttag acttcagcaa agccgatgcc aaaataagcc aatacgatct caacgctatt
      301 gaagcggctt gccagctaaa gcaacaggca gcagaggcgc aggtgacggc cttaagtgtg
      361 ggcggtaaag ccctgaccaa cgccaaaggg cgtaaagatg tgctatcgcg cggtccggat
      421 gaactgattg tggtgattga tgaccagttc gagcaggcac tgccgcaaca aacggcgagc
      481 gtacttgctg cagccgctca gaaagcaggc tttgatttga tcctctgtgg cgatggttct
      541 tccgaccttt atgcccagca ggttggtttg ctggtaggcg aaatccttaa tattccggca
      601 gttaacggcg tcagcaaaat tatctccctg acggcagata ccctcaccgt tgagcgcgaa
      661 ctggaagatg aaacagaaac cttaagcatt ccgctgcctg cggttgttgc tgtttccact
      721 gatatcaact ccccacaaat tccttcgatg aaagccattc tcggcgcggc gaaaaagcca
      781 gtccaggtat ggtcggcggc ggatatcggt tttaacgcag aggcagcctg gtcagaacaa
      841 caggttgccg cgccgaaaca gcgcgagcgt cagcgcatcg tgattgaagg cgacggcgaa
      901 gaacagatcg ccgcatttgc tgaaaatctt cgcaaagtca tttaattaca ggggatgcta
      961 tgaacacgtt ttctcaagtc tgggtattca gcgatacccc ttctcgtctg ccggaactga
     1021 tgaacggtgc gcaggcttta gctaatcaaa tcaacacgtt cgtcctcaat gatgccgacg
     1081 gcgcacaggc aatccagctc ggcgctaatc atgtctggaa attaagcggc aaaccagacg
     1141 agcggatgat cgaagattac gccggtgtca tggctgacac tattcgccag cacggcgcag
     1201 acggtctggt gctgttgcca aacacccgtc gcggcaaatt actggcggcg aaactgggtt
     1261 accgccttaa cgcggcggtg tctaacgatg ccagcgctgt tagcgtgcag gacggtaaag
     1321 cgacagtgaa acacatggtt tacggcggtc tggcgattgg cgaagaacgc attgccacgc
     1381 cgtatgcggt actgaccatc agcagcggca cgttcgatgc ggctcagcca gacgcgtcac
     1441 gcactggcga aacgcacacc gtggagtggc aggctccggc tgtggcgatt acccgcacgg
     1501 caacccaggc gcgtcagagc aacagcgtcg atctcgacaa agcccgcctg gtggtcagcg
     1561 tcggtcgcgg tattggcagc aaagagaaca ttgcgctggc agaacagctt tgtaaggcga
     1621 taggtgcgga gttggcctgt tctcgtccgg tggcggaaaa cgaaaaatgg atggagcacg
     1681 aacgctatgt cggtatctcc aacctgatgc tgaaacctga attgtacctg gcggtgggga
     1741 tctccgggca gatccagcac atggttggcg ctaatgcgtc gcaaaccatt ttcgccatca
     1801 acaaagataa aaatgcgccg atcttccagt acgcggatta cggcattgtt ggcgacgccg
     1861 tgaagatcct tccggcactg accgcagctt tagcgcgttg atccactctg gcagggctgc
     1921 attccggccc tgccgctgac agggagctct tatgtccgaa gatatctttg acgccatcat
     1981 cgtcggtgca gggcttgccg gttcggttgc cgcactggtg ctcgcccgcg aaggtgcgca
     2041 agtgttagtt atcgagcgag gcaattccgc aggtgccaag aacgtcactg gcgggcgtct
     2101 ctatgcccac agtctggaac acattattcc tggtttcgct gactccgccc ccgtagaacg
     2161 cctgatcacc catgaaaaac ttgcgtttat gacggaaaag tcagcgatga ctatggacta
     2221 ctgcaatggc gacgaaacct ctccatccca gcgttcttac tccgttttgc gcagtaaatt
     2281 tgatgcctgg ctgatggagc aggccgaaga agcgggcgcg cagttaatta ccgggatccg
     2341 cgtcgataac ctcgtacagc gcgatggcaa agtcgtcggt gtagaagccg atggcgatgt
     2401 gattgaagcg aaaacggtga tccttgctga tggggtgaat tccatccttg ccgaaaagct
     2461 ggggatggca aaacgcgtta aaccgacgga tgtggcggtt ggcgtgaagg aactgatcga
     2521 gttaccgaag tcggttattg aagaccgttt tcagttgcag ggtaatcagg gcgcggcttg
     2581 cctgtttgcg ggatcaccca ccgatggcct gatgggcggt ggcttccttt ataccaatga
     2641 aaataccctg tcgctggggc tggtttgtgg tctccatcat ctgcatgacg caaaaaaatc
     2701 ggtgccgcaa atgctggaag atttcaaaca acatccggcc gttgcaccgc tgatcgcggg
     2761 cggcaagctg gtggaatatt ccgctcacgt agtgccggaa gcaggcatca acatgctgcc
     2821 ggagctggtc ggtgacggcg tattgattgc cggtgatgct gccggaatgt gtatgaacct
     2881 cggttttacc attcgcggta tggatctggc gattgccgcc ggggaagcag cagcaaaaac
     2941 tgtgctttca gcgatgaaaa gcgacgattt cagtaagcaa aaactcgcgg aatatcgtca
     3001 gcatcttgag agtggcccgc tgcgcgatat gcgcatgtac cagaaactac cggcgttcct
     3061 tgataaccca cgcatgttta acggctaccc ggaactggcg gtgggcgtgg cgcgtgacct
     3121 gttcaccatt gacggcagtg cgccggaact gatgcgcaag aaaatcctcc gccacggcaa
     3181 gaaagtgggc ttcatcaatc tgatcaagga tggcatgaaa ggagtgaccg ttttatgact
     3241 tctcccgtca atgtggacgt caaactgggc gtcaataaat tcaatgtcga tgaagagcat
     3301 ccgcacattg ttgtgaaggc cgatgccgat aaacaggccc tggagctgct ggtgaaagcg
     3361 tgccccgcag gtctgtacaa gaagcgggat gacggcagtg tgcgcttcga ttacgccgga
     3421 tgtctggagt gcggcacctg tcgcattctg gggctggggt cggcgctgga acagtgggaa
     3481 tacccgcgcg gcacctttgg tgtggagttc cgttacggct gatgttggtt tgatacgtaa
     3541 tgccgtactg actctcattg caaaaaaaca ggaataaccc atgcaaccgt ccagaaactt
     3601 tgatgatctt aaattctcct ctattcaccg ccgcattttg ctgtggggaa gcggtggtcc
     3661 gtttctggat ggttatgtac tggtaatgat tggcgtggcg ctggagcaac tgactccggc
     3721 gctgaaactg gacgctgact ggattggctt gctgggcgcg ggaacgctcg ccgggctgtt
     3781 cgttggcaca tcgctgtttg gctatatctc cgataaagtc ggacggcgca aaatgttcct
     3841 cattgatatc atcgccatcg gcgtgatatc ggtggcaacg atgtttgtct catcccccgt
     3901 cgaactgttg gtgatgcggg tacttatcgg cattgtcatt ggtgcggatt atcccatcgc
     3961 cacctcaatg atcaccgagt tctccagtac ccgtcagcgg gcgttttcca tcagctttat
     4021 tgccgcgatg tggtatgtcg gcgcgacctg cgccgatttg gtcggctatt ggctttatga
     4081 tgtggaaggc ggctggcgct ggatgctggg tagcgcagcg attccctgtt tgttgatttt
     4141 gattggtcga ttcgaactgc ctgaatctcc ccgctggttg ttacgcaaag ggcgagtaaa
     4201 agagtgcgaa gagatgatga taaaactgtt tggcgaaccg gtggctttcg aggaagagca
     4261 gccgcagcaa acccgttttc gcgatctgtt taatcgccgc cattttcctt ttgttctgtt
     4321 tgttgccgct atctggacct gccaggtgat ccccatgttc gccatttaca cctttggccc
     4381 gcaaatcgtt ggtttgttgg gattgggggt tggcaaaaac gcggcactgg ggaacgtggt
     4441 gattagcctg ttctttatgc tcggctgtat tccgccgatg ctgtggctaa acactgccgg
     4501 acggcgtcca ttgttgattg gcagctttgc catgatgacg ctggcgctgg cggttttggg
     4561 gctgatcccg gatatgggga tctggctggt agtgatggcc tttgcggtgt atgccttttt
     4621 ctctggcggg ccgggtaatt tgcagtggct ctatcctaat gaactcttcc cgacggatat
     4681 ccgcgcctct gccgtgggcg tgattatgtc cttaagccgt attggcacta ttgtatcgac
     4741 ctggacactg ccgatcttta tcaataatta cggtatcagt aacacgatgc taatgggggc
     4801 gggtatctcg ctatttggct tgttgatttc cgtagcgttt gccccggaga ctcgagggat
     4861 gtcactggcg cagaccagca atatgacgat ccgcgggcag agaatggggt aatttgttca
     4921 gatttctctc ttttctgaat caatattatt gactataagc tgcgtgaata tatgactaca
     4981 ctttgtggga aaacaaaggc gtaatcacgc gggctaccta tgattcttat aatttatgcg
     5041 catccatatc cgcatcattc ccatgcgaat aaacggatgc ttgaacaggc aaggacgctg
     5101 gaaggcgtcg aaattcgctc tctttatcaa ctctatcctg acttcaatat cgacattgcc
     5161 gccgaacagg aggcgctgtc tcgtgccgat ctgatcgtct ggcagcaccc gatgcagtgg
     5221 tacagcattc caccgcttct taaactttgg atcgataaag ttttctcgca cggctgggct
     5281 tacggtcacg gcggcacggc gctgcacggc aaacatttgc tgtgggcggt gacgaccggc
     5341 ggcggggaaa gccattttga gattggtgcg catccgggct ttgatgtgct gtcgcagccg
     5401 ctacaggcga cggcaatcta ctgcgggctg aactggctgc caccgtttgc catgcactgc
     5461 acctttattt gtgacgacga aaccctcgaa gggcaggcgc ggcactataa gcaacgtctg
     5521 ctggaatggc aggaggcaca tcatggatag ccatacgctt attcaggcgc tgatttatct
     5581 cggttcggcg gcgctgattg ttcccattgc ggtacgtctc ggtctgggat cggttcttgg
     5641 ctacctgatc gccggctgca ttattggccc gtgggggctg cgactggtga ccgatgccga
     5701 atctattctg cactttgcag agattggcgt ggtgctgatg ctgtttatta tcggcctcga
     5761 actcgatcca caaaggctgt ggaagctgcg tgcggcagtg ttcggcggtg gcgcattgca
     5821 gatggtgatt tgcggcggcc tgctggggct gttttgtatg ttacttgggc tgcgctggca
     5881 ggtcgcagaa ttgatcggca tgacgctggc gctctcatca acggcgattg ccatgcaggc
     5941 gatgaatgaa cgcaatctga tggtgacgca aatgggccgc agagcctttg cggtgctgct
     6001 gttccaggat atcgcggcga tcccactggt ggcgatgatc ccgctactgg cagcgagtag
     6061 tgcctcgacg acgatgggcg catttgttct ctcggcgtta aaagtggcgg gcgcgctggt
     6121 gctggtggta ttgctggggc gctatgtcac gcgtccggca ctgcgttttg ttgcccgctc
     6181 tggcttgcgg gaagtgttta gtgccgtggc gttattcctc gtgtttggct ttggtttgct
     6241 gctggaagag gtcggcttgt cgatggcgat gggcgcgttt ctggcgggcg tactgctggc
     6301 aagctcggaa taccgtcatg cgctggaaag cgatatcgaa ccatttaaag gtttgctgct
     6361 ggggctgttt ttcatcggcg ttggcatgtc gatagacttt ggcacgctgc ttgaaaatcc
     6421 gttgcgcatt gtcattttgc tgctcggttt cctcatcatc aaaatcgcca tgctgtggct
     6481 gattgcccgg ccgttgcagg tgccaaataa acagcgtcgc tggtttgcgg tgctgttagg
     6541 gcagggcagt gagtttgcct ttgtagtatt tggtgcggcg cagatggcga atgtgctgga
     6601 gccagagtgg gcgaaatcgc tgaccctggc ggtggcactg tcgatggcgg caacgccgat
     6661 tctgctggtg atcctcaatc gccttgagca atcttctact gaagaggcgc gtgaagccga
     6721 tgagatcgac gaagaacagc cgcgggtaat cattgccgga ttcggtcgtt ttgggcagat
     6781 aaccggacgt ttactgctct ccagcggggt gaaaatggtg gtgctcgatc acgacccgga
     6841 ccatatcgaa acgctgcgta aattcggtat gaaagtgttt tatggcgatg ccacgcggat
     6901 ggatttactg gaatctgccg gagcggcgaa agcggaagtg ctgattaacg ccatcgacga
     6961 tccgcaaacc aacctgcaac tgacggagat ggtgaaagaa catttcccac atttgcagat
     7021 tattgcccgc gcccgcgatg tcgaccacta cattcgtttg cgtcaggcag gcgttgaaaa
     7081 gccggagcgt gaaaccttcg aaggtgcgct gaaaaccggg cgtctggcac tggaaagttt
     7141 aggtctgggg ccgtatgaag cgcgagaacg tgccgatgtg ttccgccgct ttaatattca
     7201 gatggtggaa gagatggcaa tggttgagaa cgacaccaaa gcccgtgcgg cggtctataa
     7261 acgcaccagc gcgatgttaa gtgagatcat taccgaagac cgtgaacatc tgtcattaat
     7321 tcaacgacat ggctggcagg gaaccgaaga agggaaacat actggcaata tggcggatga
     7381 accggaaacg aagccctcat cctaataaag agtgacgtaa atcacgcttt acagctaact
     7441 gtttgttttt gtttcattgt aatgcggcga gtccagggag agagcgtgga ctcgccagca
     7501 gaatataaaa ttttcctcaa catcatcctc gcaccagtcg acgacggttt acgctttacg
     7561 tatagtggcg acaatttttt ttatcgggaa atctcaatga tcagtctgat tgcggcgtta
     7621 gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc
     7681 gcctggttta aacgcaacac tttaaataaa cccgtgatta tgggccgcca tacctgggaa
     7741 tccatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg
     7801 gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca ttgcggcgtg tggtgacgta
     7861 ccagaaatca tggtgattgg cggcggtcgc gtttatgagc agttcctgcc gaaagcgcaa
     7921 aaactgtatc tgacgcatat cgacgcggaa gtggaaggcg acacccattt cccggattac
     7981 gagccggatg actgggaatc ggtattcagt gaattccacg acgccgatgc gcagaactct
     8041 cacagctatt gctttgagat tctggagcgg cggtaatttc gtatattaga attccggcat
     8101 cactgatacg tgcagatatc cgtgtatact agacgtataa attgtacagg agcacgagat
     8161 catgactgca aaacgtacca cacaaagtgt gaccgtcacc gtcgaccgtg agttagtcaa
     8221 tcgcgctcgt gatgcaggct taaatatgag cgccaccctt acggttgcgc tcaatgctga
     8281 acttaaaaaa catgcagcaa cacgttggcg tgaagagaac gcagaagcta tcgctgcgtt
     8341 aaatcaattg gctgatgaaa ccggatgttt ctctgatgag taccggagct tctagcgatg
     8401 caatttacgg tataccgcag tcgcggcagg aacgccgcgt ttccctttgt tattgatgtt
     8461 accagcgaca ttattggtga gattaatcgc cgtatcgtta ttccattaac acctattgaa
     8521 cgatttagcc gtattcgccc accagaacgg cttaacccaa tccttttact gatagacggc
     8581 aaagaatatg tgctcatgac gcatgagact gcaactgttc cagttaacgc gctgggaacg
     8641 aaattttgcg atgccagcgc gcaccgaacg ttgattaaat gagcattaga ttttatgctc
     8701 gacgggattt aagacgctac cgcctctccc tcactcaaat ccttatgccg gttcgacggc
     8761 tggacaaaat actgtttatc ttcccagcgc aggcaggtta atgtaccacc ccagcagcag
     8821 ccggtatcca gcgcgtaaat accttccggc gtacctttgc cttccagcga tgcccagtga
     8881 ccaaaggcga tgttgtactc ttcagcgacg gggccaggaa tcgcaaacca cggtttcagt
     8941 ggggcagggg cctcttccgg cgattctttg ctgtacatat ccagttgacc gttcgggaag
     9001 caaaaacgca tacgggtaaa ggcgttggtg ataaaacgca gtcttcccag cccccgcaat
     9061 tccggtgacc agttatttgg catatcgccg tacatggcat caaggaagaa gggataggag
     9121 tcactcgaca gcaccgcttc tacatcgcgc gcgcactctt tggcggtctg cagatcccac
     9181 tgcggcgtga tcccggcgtg ggccatcacc aactttttct cttcgtcgat ttgcagcaaa
     9241 ggctggcgac gcagccagtt aagcagctcg tcggcatccg gcgcttccag cagcggtgtc
     9301 aggcgatctt tcggtttatt gcggctgatc ccggcaaata ccgccagcag atgcagatcg
     9361 tgattaccca gcaccagacg tacgctgtcg cctaaggatt tcacatagcg cagaacatcc
     9421 agcgaacccg ggccgcgcgc gaccagatcg cccgtcagcc agagggtatc tttcccaggg
     9481 gtaaattcta ctttatgcag caatgcgatc agttcatcgt aacaaccatg aacgtcgcca
     9541 ataaggtatg tcgccatatt cttttaatga atgagtgtgg gaacggcgag tcggaatacg
     9601 ggaatgtcga tgctgaaagg gacgccattt tcatcgatca tttcgtagtg accctgcatg
     9661 gtgcccagcg gggtttcaat gattgcaccg ctggtgtact ggtactcttc gccaggcgcg
     9721 ataagtggct ggacgccaac cactccttcg ccctggactt cggtttcacg gccattgcca
     9781 ttggtgatca gccagtaacg ccccaacaac tgcactggcg ctcgccccag attgcgtatg
     9841 gttacggtat aagcaaaaac gtaacgttca ttatcaggtg aagattgggc ctcaatgtag
     9901 acgctttgaa cctgaataca cactcggggc gaattgatca tcgttaactc tcctgcaaag
     9961 gcgcgttctc cgccagatag ttcgccatct ggcaatattg cgcgacagag atattttccg
    10021 ctcgcatcgc cgggtcgatc cccattcccg ttaacacccc gacgctaaac aggttgccga
    10081 ggctgttacg aatggtttta cgacgctggt taaaggcttc ggtggtgatg cggctcaaca
    10141 cgcgaacatc tttaaccggg tgaggcatcg ttgcatgagg aaccaggcgc acgacggcgg
    10201 aatccacttt gggtggtggt gtaaaggctg acggcggtac ttccagcacc gggatcacgt
    10261 tgcaatagta ttgcgccatg acgcttaatc gaccatacgc cttgctgttc ggtcctgcaa
    10321 ccagacgatt caccacttct ttttgcaaca taaagtgcat gtcggcaatg gcatcagtat
    10381 agctaaacag atggaacatc aacggcgtgg agatgttata aggcaggttg ccgaaaacac
    10441 gcagcggctg gcccattttc gcggccagtt caccaaagtt aaaggtcatc gcatcctgct
    10501 gataaatcgt cagtttcggg cctaagaatg gatgcgtttg cagacgtgcc gccagatcgc
    10561 ggtcaagttc gatgaccgtc agctggtcca gacgttcgcc gaccggttcg gtcaatgccg
    10621 ccagaccggg gccgatttcg accatcgcct ggcccttctg cgggttaatg gcagaaacaa
    10681 tgctgtcgat cacgaactga tcgttgagaa agttttgccc gaagcgttta cgggctaagt
    10741 ggccctggtg gactcgatta ttcattgggt gttaacaatc attttgatgg cgagattaag
    10801 cgccgtaata aaactgccga catcggcttt accacgtccc gccagttcaa gcgcggtgcc
    10861 gtggtccact gatgtgcgaa taaagggcag gcccagcgta atgttcacac cgcgcccgaa
    10921 gccctggtat tttagcacgg gaagaccctg atcgtggtac atcgccagca cggcgtcggc
    10981 gttatcgaga tatttcggct gaaacagggt atcggcaggc agcggcccgt tgagtttcat
    11041 cccctgttcc cgcagctcgt cgagtaccgg aataatggtg tctatctctt ccgtacccat
    11101 atgaccgcct tcgcccgcgt gcggattcag cccgcagacc agaatgcgcg gttcggcaat
    11161 accaaatttg gtccgcaaat cgtgatgcaa aatagcaatc acttcgtgca aaagggcagg
    11221 ggtgatagca tctgcgatat cgcgcagcgg taaatgcgtc gttgccagcg ccacgcgaag
    11281 ttcttcggtc gccagcatca tcaccacttt tttcgcctgc gaacgctctt cgaaaaactc
    11341 ggtatgaccg gtaaacggaa tgcctgcgtc gttaatgacg cctttatgca cggggcctgt
    11401 gatcagcgca gcaaattcac cgttcagaca gccatcacac gctcgcgcca gcgtttccac
    11461 cacatagtgc ccattttcaa ccgctaacag ccccacagtg acagattcac gtagcgcgac
    11521 aggaagtagc gttaatgtgc ccgcagtttg cggttgtgca ggggagttgg gggaataagg
    11581 gcggagggtg agcggcaaac cgagcatcgc tgcccggtcg gtaaggagag tggcatcggc
    11641 acaaacaacc agttcgaccg gccactcacg ctgtgcaagc tggacaatta agtccgggcc
    11701 aatcccggcg ggctcgccgg gagtgatcac aacacgttgg gttttaacca ttagttgctc
    11761 aggattttaa cgtaggcgct ggcacgttgt tcctgcatcc agcttgctgc ttcttccgag
    11821 aacttacggt tcatcagcat gcggtatgca cgatctttct gtgccgcgtc ggttttatcg
    11881 acattacggg tatccagcag ttcgattaaa tgccagccga atgaagagtg aaccggtgca
    11941 ctcatttgac ctttgttcag gcgggtcagg gcgtcacgga aggccggatc gaaaatatct
    12001 ggtgtagccc agccgagatc accgccctgg ttagcagagc ctggatcctg agagaactct
    12061 ttcgctgcgg cagcaaaagt cgttttacca ctcttgatat cggcagcaat ctgttccagt
    12121 ttcacgcggg cctgttcgtc agtcatgatc ggcgacggtt tcagcagaat atggcgagca
    12181 tgaacttcgg tcaccgagat atttttgctt tcgccgcgca ggtcgttaac tttcagaata
    12241 tggaagccaa cgccggaacg aatcgggcca acaatgtcgc ctttcttcgc ggtgcttaat
    12301 gcctgggcga agatccccgg caactcctga atacggcccc agcccatctg gccgccgttc
    12361 agcgcctgct ggtcggcaga atgagcaatc gccagcttac cgaaatcagc gccgttacgc
    12421 gcctgatcga caatcgcgcg cgcctggctt tccgcttcgt tcacctgatc agaggtcggg
    12481 ttttccggca gcgggatcag gatgtggctc aggttcagct cagtgctggc gtcgttttgg
    12541 ttacccactt gctgcgccag ggattcgact tcctgcggca ggatggtgat gcgacgacgc
    12601 acctcgttgt tacgcacttc agagataatc atctctttgc ggatctggtt acgataggtg
    12661 ttgtagttca gaccatcgta agccagacgg ctgcgcatct gatccagcgt catgttgttc
    12721 tgtttcgcga tgttagcaat cgcctgatcc agctgctcat cggagatttt cactcccatt
    12781 ttctgcccca tctgcaggat aatttgatcc atgatcaaac gttccatgat ttggtggcgc
    12841 agcgtcgcgt catcaggaag ttgttgcctt gcctgagcag cgttcagttt taccgactgc
    12901 attaatccat caacgtcgct ttccagcacg acgccgttat tgacgacggc tgcgacttta
    12961 tcgactacct ggggggcagc gaaactggta ttcgcgatca tggcgatacc gagaagcagc
    13021 gttttccagt tcttcatact ttttccattt caattaaccg cactgcggat tacgtggtaa
    13081 atcaacaaat cacaaagtgt tttgatacgg cagaatgttc gaacgcagca tctcttgcgt
    13141 acccagaccg tagttggagc tcaggccgcg aagttcgatg ttaaagccga ttgcgttgtc
    13201 atataccgca tgttgtttat cgttatccca accgttcagc ttccgctcgt aaccgacgcg
    13261 aatcgcatag cagcaggagc tgtattgcac acctaacata gagtcggctt gcttgttcgc
    13321 attggtgtcg tagtagtagg ccccaacaat ggaccaacga tcggcaattg gccagctggc
    13381 gacggcacct acctgcgaaa taccattctt atattgctca gcagtggaat agtacttagg
    13441 cagcgtagcc tgaatatatt ccgggctggc gtaacggtaa ttcagctgta ccagacggtc
    13501 ttcatcacga cggtattcaa tgctggagtt actggtcgct acgttatcca gacgtgtatc
    13561 gtactgaatc ccgccacgca atccccaacg atcggagatg cgccagtaag tatcgcctgc
    13621 ccataccagt gagcccgttt tgtcgtcatt ctcccatgtt atgttgtcat cgccagtgcg
    13681 agactccgtg aaatagtaga tttgaccaac ggaaatatta aaacgttcaa cggcagcatc
    13741 atcatatatg cgagatgtga caccggtcgt cacctggtta gcggaggcaa tacggtcaag
    13801 accgccgtaa gtccggtccc ggaacaggcc ggagtagtca gattgcagca gcgaagagtc
    13861 gtagttatag attttgctct gatcgcgata tggcacatac agatactgcg cgcgtggttc
    13921 cagtgtctgg gtatagcccg gcgccagcat ttccatatca cgctcgaaga ccattctacc
    13981 gtctaccttg aactgcggca ttacacggtt agcggactct gccagcctgg tcgtgtttct
    14041 ggagttatac cagtcaagat tggtttgctg ataatgggtt gccagcaact tcgcttcggt
    14101 attgatgctg ccccagttat tagagagcgg cagattgatg gtcggttcca ggtgaacacg
    14161 ggttgcttca ggcatgtcgt ctctggtgtt aacaaagtgc actgcctgac cgtaaatacg
    14221 cgtatcaaac ggaccaacat cattctggta gtagttaacg tctaactgcg gctctgccga
    14281 gtagctactg gtgttctgct cgctgaagac ctggaactgc ttggttgaaa cggtggcatt
    14341 gaagttttgc accgcatagc caacgctgaa tttttgcgtt gcgtagccgt cggtactgga
    14401 accgtatttg ttatcgaagt cattgaagta gctaggatcg ctgactttgg tgtagtcgac
    14461 gttgaaacgc cacacctgat ccatgacccc ggagtggttc cagtagaaca accaacgacg
    14521 tgaactgtca tcgttcgggt gttcatcttt atagacttta tctgaaggca gatagtccag
    14581 ttccatcaag ccagcgcccg cctgggagag gtagcggaat tcgttctccc acatgatgtt
    14641 gccacgacga tgcatataat gcggcgtgat ggtggcatcc atatttggcg cgatgttcca
    14701 gtaatatggc aggtagaact caaagtagtt ggtggtggtg tacttggcgt tcgggatcaa
    14761 gaaaccagag cgacgtttgt cacccaccgg caactgcaaa tacgggctat aaaagatcgg
    14821 taccggaccc accttaaagc gggcgttcca gatctccgca acttgttctt cgcggtcatg
    14881 aataatttcg ctacctacca cgctccaggt gtcagaaccc ggcagacagg aggtaaagct
    14941 accgttatcc agaatggtat agcggttttc gccacgttgt ttcatcaggt ccgctttacc
    15001 gcgcccctgg cgacccacca tctggtaatc accttcccag acgttggtat ctttggtgtt
    15061 cagattcgcc cagcctttcg gccctttgag gatcacctgg ttatcgtcgt aatggacatt
    15121 accgagcgca tcaacggtac gtaccggctc cggttgtcct ggtgcctctt tttgatggag
    15181 ctgcacttcg tcggcctgca gacggctgtt accctgcatg atatccacgc tgccagtaaa
    15241 cacggcgtca tccggggagt cccctttcgc gtggtcagca ttgatagtca cgggtaagtc
    15301 attggtatcg ccctgtacca gaggacggtc atagcttggc acgcccaaca tgcactgtga
    15361 ggcgaggtcg gctgccagtc cctgttgact ataaagggcg gtggcaatca tggtggccag
    15421 gagagtgggg atacgttttt tcatacgttg attttattgt tccatcatcg gtaacgttgc
    15481 gcgtgacaaa cggtcagaga ctaacgtact cgtcatctct acgctagtgt taatcctgtc
    15541 cgaatagcgt cagtggtgtt aggcacggca ttgaatgaca ggtatgataa tgcaaattat
    15601 aggcgatgtc ccacaattga ccgcagccgg aaaacggtaa aagcaccttt atattgtggg
    15661 agatagccct gatatccgtg tgtcgatttg gggaatatat gcagtattgg ggaaaaatca
    15721 ttggcgtggc cgtggcctta ctgatgggcg gcggcttttg gggcgtagtg ttaggtctgt
    15781 taattggcca tatgtttgat aaagcccgta gccgtaaaat ggcgtggttc gccaaccagc
    15841 gtgagcgtca ggcgctgttt tttgccacca cttttgaagt gatgggccat ttaaccaaat
    15901 ccaaaggtcg cgtcacggag gctgatattc atatcgccag ccagttgatg gaccgaatga
    15961 accttcatgg cgcttcccgt actgcggcgc aaaatgcgtt ccgggtggga aaatcagata
    16021 attacccgct gcgcgaaaag atgcgccagt ttcgcagtgt ctgctttggt cgttttgact
    16081 taattcgtat gtttctggag atccagattc aggcggcgtt tgctgatggt tcactgcacc
    16141 cgaatgaacg ggcggtgctg tatgtcattg ccgaagagtt aggcatctcc cgcgcccagt
    16201 ttgaccagtt tttgcgcatg atgcagggcg gtgcacagtt tggcggcggt tatcagcagc
    16261 aatctggcag tggtaactgg cagcaagcgc agcgtggccc gacgctggaa gatgcctgta
    16321 atgtgctggg cgtgaagccg acggatgatg cgaccaccat caaacgtgcc taccgtaagc
    16381 tgatgagtga acaccatccc gataagttgg tggcgaaagg tttgccgcct gagatgatgg
    16441 agatggcgaa gcaaaaagcg caggaaattc agcaggccta tgagctgata aagcagcaga
    16501 aagggtttaa ataagtttca acgtgtaggc ctgataagac gcggtaggcc ggataagacg
    16561 cgccagcgtc gcatccggcg agcagcatca gacaatcggc gcacaattgc cggatgtggc
    16621 gtgaacgcct tatccggcct accgaacttc gccagcgtga ctattaaaaa tccgctggtg
    16681 ctttaaacgt catactattg ccatacgccg gatgggtaat cgtcagcatc tctgcatgta
    16741 gcaacaaacg cggtgccatt gctctcgctt ctggtgatgc ataaaaacga tcgccgagaa
    16801 tcggatgacc cagcgccagc atatgcacac gcagctgatg cgaacgcccg gtaatcggtt
    16861 ttaacaccac tcttgccgtg ttatccgccg catattccac cacttcatac tccgtctgcg
    16921 caggtttacc tgtttcgtaa cagactttct gtttcgggcg gtttggccag tcgcaaatca
    16981 gcggcagatc caccagacct tccgcagggg atggatgccc ccagacgcgg gccacatact
    17041 gttttttcgg ctcgcgctcg cggaactggc gttttaactc ccgctccgcg gctttggtca
    17101 gcgccactac aatcacgccg ctggtagcca tatccagacg atgcaccgat tctgcctgcg
    17161 gataatcacg ctgaatgcgc gtcatcacgc tgtctttgtg ctcttccaga cgacccggca
    17221 cagacaacaa accgctcggc ttgttgacca ccataatatg gtcatcctga tacaggacaa
    17281 ccagccaggg ttcctgcggc ggattgtagt tttccatccc catcttcggc tccgttactg
    17341 atgcgtcaca acgatcaaac gcagggcatc cagacgccag cctgcctgat ccaggctttc
    17401 cattacctgc tgacggttgc tctcaatggc ggtcagttcg tcgtcacgaa tgttcgggtt
    17461 aactgcacgc agcgcttcca gacgagacag ctcggcagac agtttttcgt cggcttcgtt
    17521 acgcgctgca tcaatcaatg cacgggcaga tttctcgatc tgcgcttccc ccagctgaag
    17581 gatagcgtga acatcctgct gcaccgcgtt aaccagcttg ctgccggtgt gacggttaac
    17641 cgcgttaagc tgacggttaa aggtttcaaa ttctacctgc gccgccaggt tgttgccgtt
    17701 tttatccagc agcatacgta ccggcgtcgg tggcaggaag cggttgagct gcaactgctt
    17761 cggagcctgg gcttcaacca cataaatcag ttccaccaac agcgtaccta ccggcaacgc
    17821 tttgtttttc aacagtgaaa tcgtgctgct accggtatcg ccagaaagga tcagatccag
    17881 accgttgcgg atcagcggat gctcccaggt aataaactgt gcatcttcac gcgccagcgc
    17941 cacttcacgg tcgaaggtga tggtgatgcc atcttccgac aggccaggga agtccggcac
    18001 cagcatatga tcggacggcg tcagcacgat catgttgtcg ccgcgatcgt cctgattgat
    18061 accgataata tcgaacaggt tcatggcgaa ggcgatcaga ttggtatcgt catcctgctc
    18121 ttcaatgctt tctgccagtg cctgggcttt ttcgccaccg ttggaatgga tctccagcag
    18181 gcggtcacgc ccctgttcca gttgtgcttt cagcgcttcg tgctgctcgc ggcagttttt
    18241 gattagatcg tcaaagcctt cggtctcatc cggtgaagcc agatagttaa tcagatcgtt
    18301 gtatacgcta tcgtaaatag tgcgtccggt cgggcaggtg tgctcgaacg catccagacc
    18361 ttcgtgatac cagcgcacca gcaccgactg agccgttttt tccagataag gcacatggat
    18421 ctgaatatcg tgcgcctgac cgatacgatc cagacgccca atacgctgct ccagcagatc
    18481 cgggttgaat ggcaggtcaa acatcaccat gtggctggcg aactggaagt tacgtccttc
    18541 agaaccgatt tccgagcaca gcagtacctg cgcaccggtg tcttcttcag caaaccaggc
    18601 ggcagcgcgg tcacgttcga taatcgacat gccttcgtgg aacactgcgg cgcgaatacc
    18661 ttcacgttcg cgcagtacct gctccagttg cagcgcagtg gccgctttgg cgcagatcac
    18721 cagcactttc tgagagcgat gactggtcag gtagcccatc agccattcaa cgcgcggatc
    18781 gaagttccac caggtggcgt tatcgccttc aaattcctga taaatacgct ccggatagag
    18841 catatcgcga gcgcgatctt ccgcactttt gcgtgcgccc ataatgccgg agactttaat
    18901 agcggtctga tactgcgtcg gcagtggcag cttaatagta tgcagctcgc gtttcgggaa
    18961 gcctttcaca ccgttacggg tgttacggaa cagcacgcgg ctggtaccgt ggcgatccat
    19021 cagcatcgaa accagttcct gacgggcgct ctgggcatct tcgctgtcgc tgtttgctgc
    19081 ttgcaacagc ggctcgatat cctgctcgcc gatcatctca ccgagcatgt tcagttcgtc
    19141 attgctgagt ttgttacccg ccagcagcat ggcaacggcg tccgcaaccg gacgataatt
    19201 tttctgctct tcaacgaatt gcgcaaaatc gtggaaacgg ttcgggtcca gcagacgcag
    19261 acgggcgaag tggctttcca tccccagctg ttccggggtt gcggtcagca gcagaacgcc
    19321 cggcacgtgc tctgccagtt gttcaatggc ctgatattca cggctcggcg catcttcgct
    19381 ccacaccagg tgatgcgctt catcgaccac cagtagatcc cactcggctt cacagagatg
    19441 ttccagacgc tgtttgctac gacgggcaaa atccagcgag caaatcacca gctgctcggt
    19501 atcgaacggg ttgtaagcat cgtgctgagc ttcggcataa cgctcatcat caaacagagc
    19561 aaagcgcagg ttgaaacggc gcagcatttc taccagccac tgatgctgta aggtttccgg
    19621 gacgataatc agcacacgtt cagcagcgcc agagagcagt tgctgatgca ggatcatccc
    19681 ggcctcaatg gttttcccta aacccacttc gtcagccaac aggacgcgcg gcgcgtggcg
    19741 gcgaccaaca tcatgagcga tgttgagctg gtgcgggatc aggctggtac gctgaccgcg
    19801 caggccgctg tacggcatac ggaactgttc gctggaatac ttacgcgcgc gataacgcag
    19861 cgcaaagcgg tccatacggt caatctgccc ggcaaacaga cggtcctgcg gtttgctgaa
    19921 caccagtttg ctatcaagga aaacttcacg cagggccacg ccggactctt cagtatcaag
    19981 gcgagtaccg atataggtca gcaagccatt ttcttctttt acttcttcga cttgcatctg
    20041 ccagccgtca tggctggtaa tggtatcacc agggttgaac atcacgcggg tcacggggga
    20101 atcactgcgt gcgtacagac ggttttcacc agtagatggg aaaagtaaag tgacagttcg
    20161 cgcatccacc gcgacaacgg ttccaagtcc caattcgctt tctgtatcgc tgatccagcg
    20221 ttgaccaagt gtaaaaggca tatgtgttcg gctctatatc tttaattgca ggcaataacc
    20281 acccgctacc gtgcttatga ggtagtggtg ttattcaggt ccaggaatgg aaagggcgct
    20341 atggtactgg atggcaaagc attcgtcacg catcaaaatg gtatctggcg aactcttttt
    20401 tttgctcaaa atagcccaag ttgcccggtc ataagtgtag caaaattatc ctcaataaaa
    20461 gggagtattc cctccgctac gggttgtagc tggcgggtca gatagtgttc gtaatccagt
    20521 ggtgaacgtt ggtagtccag cggctccggg ccgttggtgg tccatacgta cttaatggtg
    20581 ccgcggttct gatattgcaa ggggcgaccg cgcttatggt tttcttcatc ggcaaggcga
    20641 gcggcgcgta catgaggcgg cacattacgc tgatattcgc tcagcggacg gcgaaggcgt
    20701 ttacggtaaa ccagccgcgc atccagttca cccgccatca gtttgtcgat ggtttcacgc
    20761 acgtattcct gatatggctc gttgcggaag atacgcaggt aaagctcctg ctgaaactgc
    20821 tgggccagtg gcgtccagtc ggtgcgcacg gtttccagcc ctttaaacac catccgctgt
    20881 ttgtcgccct cctgaatcag tccggcgtag cgctttttgc tgccggtatc ggccccgcga
    20941 atggttggca tcagaaaacg gcagaaatgg gtttcatact ccagttccag tgcgctggtc
    21001 agccgttgtt tttgcagcgt ttccgcccac caggcgttaa cgtgctgcac cagtgcacga
    21061 ccgattttcg ctgcttcttc ttccgaatgt gcacctttca gccagacaaa cgttgagtcg
    21121 gtatcgccgt agataacgtc atagccctgc gcttcaatca acgttttggt ttgccgcatg
    21181 atctgatgac cacgcatggt gatcgacgat gccaggcgtg gatcgaagaa acggcaggcg
    21241 gtggtgccga gcacaccata aaaggcattc ataatgatct tcagcgcctg cgaaagcggc
    21301 ttattaccct gacgtttggc ttcatcgcgc ccgtgccaga tgttagtcac aatctccggc
    21361 aggcaatgtt tctcccgtga gaaccacgca tcgagaaaac cttcggtact gtgctctgga
    21421 tcaggctgcg ccatgccttc caccagcccg acgggatcaa tcagaaaggt gcggatgatc
    21481 gacgggtaca ggcttttgta gtccagcacc agcaccgaat cataaagccc aggtcgcgaa
    21541 tccatcacgt agccgccagg gctggcgtgc ggcggcactt cgccgagatt aggcgcgaca
    21601 taaccagcgc gatgcatacg gggaaaatag agatgaccaa acgctgccac cgaaccgccg
    21661 tgtcgatcca ccggcaggcc gttcaccgtc gcccgttcga gcaaaaacgg cataatttca
    21721 gttttgtgga agatctgcgt caccagctcg caatctttca ggttataagt tgccagcgca
    21781 ggtttatctt cggcgaaacg gcggtcaatt tcgtccattc gatcccacgg gttatcgata
    21841 gattttcctt cgcctaataa ctcctgagcg acggtttcca gcgagaatga agagaaattc
    21901 cagaacgcgg atttcagcgc ctcgataccg tcgataatta gccgaccttt aacctgagca
    21961 aaaaacacac cgtttttaaa gccgtgctcg cgccactcca gctcactgtt atcacgcccc
    22021 agccgcagcg gaatacggta acgctcggca tgtttttgca gcattcgcag atcgaactgc
    22081 accacgttcc aaccgatgat cacatcagga tcgtagttgg caaaccaggc gttaagtttt
    22141 tccagtagca gcgggcggct ggcgacgtat tccagttcaa aatcgagcgc ggaggcgtcg
    22201 ccattctctg gccccagcat ataaacgata cgctgcccgc aaccttccag gccgatgcag
    22261 tacagctcac cgtggcgggt ggtttcaata tcaatagaaa cccacttgag cggcggacga
    22321 tagtcgggat gcggtttcag acgggcatta acgatagcgc cattgcgcat atcaccctcg
    22381 acccacaccg gtgaggtgat aaatcgctcc atcagatagc gttctggcgg gcgcacatcg
    22441 gcctcgtaga cggtaacgcc accttcacgc aggcgctttt cgtaattcat caactggcga
    22501 tgggcgcgac agtaaaggcc atacaccggc tggcggtgaa aatcttttaa cgccagcggc
    22561 gtcaggcgaa agccttgttc accctgcaaa atatgctgag cgcggggaac ctgatcggca
    22621 ggaataaacg ccacggactc ttgcggcgca aggaaaacct gcaacggccc gttgtccgtc
    22681 gccagccaga aggaaacttc ggtcccttgc ggggtgtccc gccagtgtcg ggttaagata
    22741 aaacctgcct gcgccacgct gaaaatccaa taaaaaacca ggcttgatta tagcctggtt
    22801 tcgtttgatt ggctgtggtt ttatacagtc attactgccc gtaatatgcc ttcgcgccat
    22861 gcttacgcag atagtgttta tccagcagcg tttgctgcat atccggtaac tgcggcgcta
    22921 actgacggca gaatataccc atataagcga cctcttccag cacgatggca ttatgcaccg
    22981 catcttcggc atttttgccc catgcaaatg ggccgtgaga atggaccagg acgccgggca
    23041 tttgcgctgc atcgataccc tgtttttcga aggtttctac gatgacgtta ccggtttccc
    23101 actcatattc accgttgatt tctgcgtcgg tcattttgcg ggtgcaggga atgggaccgt
    23161 agaaatagtc ggcgtgggtg gtaccggttg ccggaattga ctgacccgcc tgcgcccaga
    23221 tggtggcgtg gcgcgagtgt gtgtgcacaa tgccgccaat ggacgggaat gcctgataga
    23281 gcagccggtg agttggcgtg tcggaggagg gctttttcgt accttcaacc acttcaccgg
    23341 tttcgatgct gaccacgacc atatcgtcag cggtcatgat gctgtagtcg acgccggaag
    23401 gtttgatcac aaagacgccg cgcccgcgat caacggcgct gacgttgccc caggtgagcg
    23461 tgaccaggtt gtgttttggc agcgccaggt tggcttctaa tacctggcgt ttgagatctt
    23521 ctaacatgtt gactccttcg tgccggatgc gatttgctta tccggcctac aaaatcgcag
    23581 cgtgtaggcc tgataagacg cgtcggcgtc gcatcaggcg ttacatacct gatgcggcta
    23641 cttagcgacg aaatccgtaa tacacttcgt tccagcgcag cgcgtcttta aacgctggaa
    23701 ggcgggtatc gttatcaatc accgtgattt caatgtcgtg catctcggcg aactggcgca
    23761 tatcgttgag gttcagcgca tggctgaaga cggtatggtg cgcgccaccg gcaaggatcc
    23821 acgcttcgga agcggtcggc agatccggtt gcgctttcca cagcgcattc gccaccggca
    23881 gtttcggcag ggagtgcggt gttttcaccg tgtcgatgca gttaaccagc agacgataac
    23941 gatcgccgag atcaatcagg ctggcgacaa tggccggacc agtttgagta ttgaagatca
    24001 ggcgagccgg gtcgtcttta ccgccaatac caaggtgctg aacgtcgagg atcggtttct
    24061 cttccacagc gatcgacggg cagacttcca gcatatggga gccgagtacc aggtcattac
    24121 ctttctcgaa gtgataggtg tagtcctcca taaaggaggt gccgccctgc agaccggttg
    24181 acatcacctt catgatgcga agcagggcgg cagttttcca gtcgccttcg cccgcaaagc
    24241 cgtagccctg ctgcatcaga cgctgtacgg ccagaccagg aagctgtttc aggccgtgca
    24301 aatcttcaaa ggtagtagtg aaagcgtgga agccaccctg ttccaggaaa cgcttcatcc
    24361 ccagctcaat acgcgccgct tccagcacgt tctgtcgttt ttcgccgtgg atttgtgtgg
    24421 caggtgtcat ggtgtagcag ctttcgtact catcgaccag cgcgttaaca tcgccgtcgc
    24481 tgatggagtt caccacctgc accagatcgc caaccgccca ggtattgacg gagaaaccaa
    24541 acttgatctg tgcggcaact ttatcgccat cggtgaccgc tacttcacgc atgttatcgc
    24601 caaaacggca gactttcaga tgacgggtat cctgtttaga gaccgcctga cgcatccagg
    24661 agccgatacg ctcatgtgct tgtttatcct gccagtgacc ggtaacaacg gcatgttgct
    24721 gacgcatacg cgcgccgatg aagccgaact cgcgaccgcc atgcgcggtc tggttcaggt
    24781 tcataaagtc catgtcgatg ctgtcccacg gcagcgccgc gttgaactgg gtgtggaatt
    24841 gcagcaacgg tttgttgagc atggtcaggc cgttgatcca cattttggcc ggggagaaag
    24901 tgtgcagcca caccaccaga ccagcgcaac gatcgtcgta attcgcgtcg cggcaaatag
    24961 cggtgatttc atccggcgtg gtgcccagcg gtttcagcac cagtttgcag ggcaacttcg
    25021 cttctgtatt cagtgcatta acaacgtgtt ccgcatgttg cgtcacctgg cgcagagtct
    25081 ccgggccgta aagatgctgg ctgccaatta caaaccacac ttcataatta tcaaaaatcg
    25141 tcattgtcgt gtccttatag agtcggaacg gcctgggcag cctgtgccgg ggcggaagtt
    25201 ggaagatagt gttgttcggc gctcatcgcc cattgctgat agcggcgata aagctgttca
    25261 aagcgttgtg cctgttcgct gcgcggttgc agggttttct ctaccgcact ggccattttt
    25321 tgctgggctg atgggatgtc tgcgtgcact ttcgcggcga cagcagcaaa aatcgccgca
    25381 ccgagcgcac agcactggtc agaggcaaca atttgcagcg ggcgattcag cacgtcgcag
    25441 caggcctgca taatgacctg gtttttccgc gcgatgccgc ccagcgccat cacgttattg
    25501 acggcgatcc cctgatcggt aaagcactcc atgattgcgc gtgcgccaaa ggcggtggca
    25561 gcaatcaaac cgccgaacag cagcggagcg tcggtagcga ggttcagatc ggtaatcacc
    25621 cctttcaggc gttggttagc gttcggtgtg cggcggccgt taaaccagtc gagcaccacc
    25681 ggtaggtgat ccagagacgg attttgagcc catacttcgg tcagcgccgg aagcagttgt
    25741 ttctggctgg cgttgatttg ctctttcagt tccggatgct gggcggcaag ctgttccagc
    25801 ggccagccga gtacgcgacc aaaccaggcg tagatatccc caaacgccga ttggcctgct
    25861 tccagaccga taaatccagg caccacgctg ccatcaacct gaccgcaaat acctttcact
    25921 gcccgctcgc caacgctctg tttgtcggca atcagaatgt cacaggtgga agtaccgata
    25981 acttttacca gtgcgttagg ctgtgcgcct gcgccaactg cgcccatatg gcagtcaaac
    26041 gcgccgccgg aaatcactac gctttcaggc aggccgagac gctgcgccca ttccgggcat
    26101 aaggtgccca ccggaatatc ggcagtccag gtatcagtga acagcgggga aggcaaatgg
    26161 cgattgagga tcgggtccag ctcatcaaag aaactggctg gcggcaggcc accccagctt
    26221 tcgtgccaca gagatttatg cccggcgctg caacgtccgc gacgaatatc ctgcgggcgg
    26281 gtggtaccgg aaagcagagc tggcacccag tcgcacagct caatccatga tgcggcagat
    26341 tgtgccacgg cgttgtcctg gcgagttaca tgcaggattt ttgcccagaa ccattcgctg
    26401 gaataaatac cgccaatatt gcgggagtag tcaacgttgc ccggcgtgtg acataagcgg
    26461 gtaatctctt ccgcttcttc aaccgcggtg tggtctttcc acaatacgaa catcgcgttc
    26521 gggttctcgg caaactccgg gcgcagcgcc aacacgttgc cgtcggcgtc aatcggtgcg
    26581 ggcgtcgagc cggttgtgtc aacgccaatc ccgaccacag ctgcgcgctg ttcgacgcaa
    26641 agctctgcaa gcacagtttt cagcgccgct tccattgact caatgtagtc acgcggatga
    26701 tgacggaact ggttattcgg ggcatcacaa aattgcccgt tctgccaacg gggataccac
    26761 tctacgctgg tggcgatctc ttcaccagtg gcgcattcca ccgccaaagc tcgcacagaa
    26821 tcactgccaa aatcgaggcc aattgcaatc gccatcgttt cactccatcc aaaaaaacgg
    26881 gtatggagaa acagtagaga gttgcgaaaa aaaacgtcag gctggatccg ctaatcttat
    26941 ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt
    27001 tctgccgtga ttatagacac ttttattacg cgtttttgtc atggctttgg tcccgctttg
    27061 ttacagaatg cttttaataa gcggggttac cggttgggtc agcgagaaga gccagtaaaa
    27121 gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg
    27181 gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa
    27241 cgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat
    27301 cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggcat
    27361 agtgaaaaac cagggacgag aatttgtctg ccgaccgggt gatattttgc tgttcccgcc
    27421 aggagagatt catcactacg gtcgtcatcc ggaggctcac gaatggtatc accagtgggt
    27481 ttactttcgt ccgcgcgcct actggcatga atgggttaac tggccgtcga tatttgccaa
    27541 taccggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca
    27601 aatcattaat gccggacaag gggaagggcg ctattcggaa ttgctggcga taaatctgct
    27661 tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc cgccgatgga
    27721 taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gtaattttga
    27781 tatcgccagc gtcgcacagc atgtctgcct gtcgccgtcg cgtctgtcgc atttgttccg
    27841 ccagcagtta gggattagcg tattaagctg gcgcgaggac cagcgcatca gtcaggcgaa
    27901 gctgcttttg agcaccaccc ggatgcctat cgccacagtc ggtcgcaatg tcggttttga
    27961 cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc cgagcgagtt
    28021 tagggctgga tgtgaataat aagaaagaag ggggagtctc t
//
LOCUS       DACADA010000053        27726 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.054, whole genome shotgun sequence.
ACCESSION   DACADA010000053 DACADA010000000
VERSION     DACADA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.054"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            95..598
                     /gene="dps"
                     /locus_tag="ISB17_002189"
                     /gene_synonym="pexB"
     CDS             95..598
                     /gene="dps"
                     /locus_tag="ISB17_002189"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAP1980122.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1002..1748
                     /gene="glnH"
                     /locus_tag="ISB17_002190"
     CDS             1002..1748
                     /gene="glnH"
                     /locus_tag="ISB17_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAP1980123.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1887..2546
                     /gene="glnP"
                     /locus_tag="ISB17_002191"
     CDS             1887..2546
                     /gene="glnP"
                     /locus_tag="ISB17_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAP1980124.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2543..3265
                     /gene="glnQ"
                     /locus_tag="ISB17_002192"
     CDS             2543..3265
                     /gene="glnQ"
                     /locus_tag="ISB17_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAP1980125.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3382..5607
                     /gene="ybiO"
                     /locus_tag="ISB17_002193"
     CDS             3382..5607
                     /gene="ybiO"
                     /locus_tag="ISB17_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAP1980126.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5604..6530)
                     /gene="rlmF"
                     /locus_tag="ISB17_002194"
     CDS             complement(5604..6530)
                     /gene="rlmF"
                     /locus_tag="ISB17_002194"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAP1980127.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6806..7066
                     /locus_tag="ISB17_002195"
     CDS             6806..7066
                     /locus_tag="ISB17_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1980128.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7331..9613
                     /locus_tag="ISB17_002196"
     CDS             7331..9613
                     /locus_tag="ISB17_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAP1980129.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9655..10332
                     /gene="ybiX"
                     /locus_tag="ISB17_002197"
     CDS             9655..10332
                     /gene="ybiX"
                     /locus_tag="ISB17_002197"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAP1980130.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10406..10672
                     /gene="ybiI"
                     /locus_tag="ISB17_002198"
     CDS             10406..10672
                     /gene="ybiI"
                     /locus_tag="ISB17_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAP1980131.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            10937..11197
                     /locus_tag="ISB17_002199"
     CDS             10937..11197
                     /locus_tag="ISB17_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1980132.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11335..12420)
                     /locus_tag="ISB17_002200"
     CDS             complement(11335..12420)
                     /locus_tag="ISB17_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAP1980133.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12560..13522)
                     /gene="ybiB"
                     /locus_tag="ISB17_002201"
     CDS             complement(12560..13522)
                     /gene="ybiB"
                     /locus_tag="ISB17_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAP1980134.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(13550..15700)
                     /gene="dinG"
                     /locus_tag="ISB17_002202"
     CDS             complement(13550..15700)
                     /gene="dinG"
                     /locus_tag="ISB17_002202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAP1980135.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15804..16004
                     /locus_tag="ISB17_002203"
                     /pseudo
     CDS             15804..16004
                     /locus_tag="ISB17_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16237..17598)
                     /gene="rhlE"
                     /locus_tag="ISB17_002204"
     CDS             complement(16237..17598)
                     /gene="rhlE"
                     /locus_tag="ISB17_002204"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAP1980136.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17827..18498
                     /gene="cecR"
                     /locus_tag="ISB17_002205"
     CDS             17827..18498
                     /gene="cecR"
                     /locus_tag="ISB17_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAP1980137.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18498..19496
                     /gene="hlyD"
                     /locus_tag="ISB17_002206"
     CDS             18498..19496
                     /gene="hlyD"
                     /locus_tag="ISB17_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAP1980138.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            19489..21225
                     /locus_tag="ISB17_002207"
     CDS             19489..21225
                     /locus_tag="ISB17_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1980139.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21218..22351
                     /locus_tag="ISB17_002208"
     CDS             21218..22351
                     /locus_tag="ISB17_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1980140.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22362..23468
                     /locus_tag="ISB17_002209"
     CDS             22362..23468
                     /locus_tag="ISB17_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1980141.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23430..23840)
                     /locus_tag="ISB17_002210"
     CDS             complement(23430..23840)
                     /locus_tag="ISB17_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980142.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            23973..24734
                     /locus_tag="ISB17_002211"
     CDS             23973..24734
                     /locus_tag="ISB17_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP1980143.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24731..25972
                     /gene="clsB"
                     /locus_tag="ISB17_002212"
     CDS             24731..25972
                     /gene="clsB"
                     /locus_tag="ISB17_002212"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAP1980144.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            25972..26928
                     /locus_tag="ISB17_002213"
     CDS             25972..26928
                     /locus_tag="ISB17_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAP1980145.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(26964..27677)
                     /locus_tag="ISB17_002214"
     CDS             complement(26964..27677)
                     /locus_tag="ISB17_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP1980146.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         6559 a         7358 c         7224 g         6585 t
ORIGIN      
        1 tgtgatagga acagccagaa tagcggaaca catagctggt gctatactta gtctcgttaa
       61 ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat tagttaaatc
      121 aaaagcgacc aatctgcttt atacccgcaa cgatgtctct gacagcgaga aaaaagcaac
      181 agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga ttaccaaaca
      241 agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc tggatggctt
      301 ccgcactgca ctgatcgatc atctggatac catggcagaa cgtgcagtgc agctgggcgg
      361 tgtagctctg gggaccactc aagttatcaa cagcaaaact ccgctgaaaa gttacccgct
      421 ggacatccac aacgttcagg atcacctgaa agagctggct gaccgttacg caatcgtcgc
      481 taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag atatcctgac
      541 cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca tcgaataaat
      601 ccatcgctga tggtgcagaa ctttagtaca cgataaaagc ggcttcctga caggaggccg
      661 ttttgttttg tagcccgcct caacgcactt atttagtgca tccatctgct atccccagct
      721 ggttaagtaa attttttgta tccacatcat cacacaatcg ttacataaag attgtttttt
      781 catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg caccagtttg
      841 gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc acgtttttgc
      901 cgcatctcga aaaatcaatg agttgcaaaa ctggcacgat tttttcatat atgtgaatgt
      961 cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag
     1021 tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg gataaaaaat
     1081 tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc gataaatacg
     1141 tgggctttga cgttgatctg tgggctgcca tcgctaaaga actgaagctg gattacgaac
     1201 tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac gtcgatctgg
     1261 cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc tctgacggct
     1321 actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg aaaagcgtga
     1381 aagatctcga cgggaaagtg gttgctgtga agagcggcac tggctccgtt gattacgcga
     1441 aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac gcctatatgg
     1501 aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt ctgtacttca
     1561 tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa gcacagcaat
     1621 acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga
     1681 aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc ggtactgaac
     1741 cgaaataata acgctgcgcc tgtaaagcgc actggcagtt ccctctcccc gccggggaga
     1801 ggattagggt gaagggcgca aacccgctcc ggggccatta attaccctga atttgattat
     1861 ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc tggcctgcca
     1921 ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc ggtctggctg
     1981 gcggtctggt gatcggattg ttggcaggtt ttgcacgcac cttcggtggt tggatagcca
     2041 accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc gtccaggtga
     2101 tgtttatcta tttcgccctg ccgatggcgt ttaacgattt acgcatcgac ccgtttactg
     2161 cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg
     2221 cggtgctgtc tattcacaaa ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt
     2281 gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg ccgccgctgg
     2341 gtaaccagtg gatcatcagc attaaagaca cttcgctgtt tattgttatc ggcgtggcgg
     2401 aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt gagatctgga
     2461 gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt ctgcgtcgtc
     2521 tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc attttggtcc
     2581 aacccaggtg ctgcacaata tcgatttgaa cattgctcag ggcgaagtcg tggtgattat
     2641 cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac tggaagaaat
     2701 cacctccggc gatctgattg tcgatggcct gaaggttaac gatccgaaag ttgatgagcg
     2761 cctgattcgc caggaagcgg gtatggtgtt ccagcagttt tacctcttcc cgcatctgac
     2821 ggcgctggaa aacgtcatgt ttggcccgct acgcgtgcgt ggcgcgaata aagaagaggc
     2881 ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagagcgtg cacatcacta
     2941 cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt
     3001 gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg aactgcgcca
     3061 tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg tgatcgtgac
     3121 ccacgaaatc ggttttgccg agaaagtagc ttcgcgcctg atctttatcg acaaaggccg
     3181 gattgcagaa gatggcgatc cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca
     3241 ggaatttttg cagcacgtct cttaacttct ctgcctccct ttcccccgaa agggaggcac
     3301 accagattcc tctcatttaa aattgcctct cccccagcat ctatacttat ctttttgctc
     3361 tgttttctca ctggaggagt catgcggtgg atcctgttca tcctcttctg cctgctgggc
     3421 gcacctgccc acgcggtatc catacccggc gttacaacca caacgacaac ggactcaacg
     3481 actgaaccgg ccccggaacc ggatatcgaa caaaaaaaag cggcctatgg cgcactggcg
     3541 gatgtgctgg ataatgacac ctcgcgtaaa gagttgatcg accagttgcg caccgtcgcc
     3601 gcgacgcccc ctgcggaacc ggtaccgaag atcgtgccgc cgacgctggt tgaagagcaa
     3661 accgtgctgc aaaaggtcac cgaagtcagc cgccattatg gtgaagccct ttccgcccgc
     3721 ttcgggcaac tttatcgcaa tatcaccggc tccccgcata agccgtttaa tccacaaacc
     3781 ttcagcaatg cgctgaccca cttttcaatg ttagcggtat tagtgtttgg tttttactgg
     3841 ctgattcgtc tgtgcgcact gccgctgtat tgcaaaatgg gccagtgggc gcggcaaaaa
     3901 aatcgtgagc gcagtaactg gttgcagctt ccggcgatga tcatcggggc gtttattatc
     3961 gacctgctgt tactggcact gacattattt gtcggccagg tattaagcga caacctgaat
     4021 gcgggcagtc gcaccatcgc tttccaacaa agtttgtttc tcaacgcctt tgccctgatt
     4081 gaatttttca aaaccgtact acgcctgatt ttttgcccaa acgtagctga actgcgcccg
     4141 ttcacgattc atgacgagac cgcccgttac tggagtcgtc gcctgagctg gttaagcagc
     4201 ctgattggct atggtctgat tgtggctgtg ccgattatct ctaatcaggt gaatgtacag
     4261 ataggtgcgc tggcgaacgt catcattatg ctgtgcatga ccgtctgggc gttgtacctg
     4321 atttttcgta ataaaaaaga gatcacccag catttgctca actttgcgga gcattcgctg
     4381 gcctttttca gcctgtttat ccgcgccttt gcactggtgt ggcactggct ggcaagcgcc
     4441 tattttatcg tgctgttttt cttttcgttg ttcgatccgg gcaacagcct gaaatttatg
     4501 atgggtgcaa cggtgcgcag cctggcgatt attggtatcg cagcatttgt ttccggtatg
     4561 ttttcccgct ggctggcgaa aaccatcacc ctctcgccac atactcagcg aaattatccg
     4621 gagctgcaaa aacggctgaa tggatggctt tcggcggcac tgaaaacagc gcgtattctg
     4681 acagtctgcg tggcggtaat gctgctgttg agcgcatggg gattgttcga tttctggaac
     4741 tggctgcaaa acggtgcggg gcagaaaacc gtagatatcc tgatccgtat cgcactcatt
     4801 cttttcttct cggcggttgg ctggacggtg ctcgccagtt tgatagaaaa ccggctggct
     4861 tcggatattc atggccgccc gctacccagc gcccgtacgc gtaccctgct aacgctgttc
     4921 cgtaacgcgc tggcggtgat tatcagtacc atcaccatca tgattgtgtt gtcggaaatc
     4981 ggcgtcaata tcgcgccatt gctggcaggt gccggtgcat tgggactggc tatctctttt
     5041 ggttcgcaaa cgctggtgaa agatattatc accggggtat ttattcagtt tgaaaacggc
     5101 atgaacactg gagatttggt gactatcggg ccgttgaccg gcactgtgga acggatgtca
     5161 attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc gtggtcttcg
     5221 ataaccacct ttgctaactt cgtccgcggt attggttcgg tagtggcaaa ctacgatgtt
     5281 gatcgccatg aagatgctga taaagccaat caggcactga aagatgcggt agcagaattg
     5341 atggaaaacg aagaaattcg cgggctggtt attggtgaac cgaattttgc cgggattgtc
     5401 ggcttaagca ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc actcaaacag
     5461 tggacggtac gctttgccct cgacagccag gtgaaaaaac atttcgacct ggcgggtgtt
     5521 cgcgcgccgg tgcagactta tcaggtgctg ccagctccgg gcgcggcccc ggctgaaccg
     5581 ttgccgccgg gggagccaac gctttaacgc tggcgattga caaaacggcg gcgctgctcg
     5641 tcgttcataa aggtccaggc tataaagcgg ctctgctttt gcccctgggc catctctttt
     5701 tttaccacct tcaccgcacc tacgtccgtc aaagcacgat acaacggcgg taagttttca
     5761 ccacgagata ccagcgaggt aaaccacatc acctgcttcg cgaagccttt gctctcttca
     5821 atcatctttt tgataaaagc gacctcaccg ccttcacacc acaactcctg ttgctgacca
     5881 ccaaagttca gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg ttcactacct
     5941 gcccgtgccg cagcggcgga atcgtggaat ggcgggttac acaaggtcgc atcgtattgc
     6001 tcgtttttat ggatgatgcc gttaaaaatc gccccactct ctttttgccg acgcagacga
     6061 atggcgcggt taagccccgg attagcactg atgatcgcct gcgcactgct taacgcctgg
     6121 ctgctggttt cactaccggt aaaacgccag ccatattcat gtacgccaat cagcggataa
     6181 atacagttcg caccaacgcc gatatccaga atgctggcat tagctggaat tgttccgctt
     6241 gcttcggcca gtaaatcggc aaggtgatga atataatccg cccggcccgg tactggtggg
     6301 cagagaaaac cgtccgggat atcccagttc gctacggcgt aaaaatgggc cagcaacgcc
     6361 ttattgagcg ccttcaccgc cagcggattg gcaaagtcta cgctttgctc cccggcgggt
     6421 gtaagcgtga ggaattgcct gagttcagga ttgacctgac aaagcgtggc gagatcgtag
     6481 cggctgtgat gacggttgcg cggatgcaac cccggtttct gggcggacat agcattctcc
     6541 tttaagcagc ggcgtaagat acccgcataa cgcgacaggg taaatatctg gctgcgttta
     6601 tcatttctaa aaaccttcac atggctatgg tttatggcaa ttcaccataa aaaacatcat
     6661 catgcatgtc attgatttgt cttattttgt aaaaacacat cgttcaaaaa ataagcgcat
     6721 ctctgtcaat tttttgcgtc attttcatcg cgcaggcgac ctacacttat ctggcactac
     6781 ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc acactactga ttgccaccgt
     6841 cctgagcgga atctctctca cggcttatgc cgcgcaaccg atgagtaacc tggacagtgg
     6901 tcaactacgg cccgccggta ccgtttcggc aactggcgca tcaaacctaa gcgatctgga
     6961 ggataaactg gcagaaaaag cgcgcgaaca aggcgcgaaa ggttatgtca ttaattccgc
     7021 aggcggaaat gaccagatgt tcggtactgc aatcatctac aaataacccc accaacgtaa
     7081 ctgcattcgc ctctggatgc agttttcgtc caatttgtaa gcaaaaaaca tcaaatatga
     7141 acgcaatgta aataaatgta tttctttttc gcgcaatggg tgatagaaaa tcgcgccaag
     7201 tgataatgct tatcaatatt attatcactt gcacgtacgc tatcacggga ttaacagtgg
     7261 catcgcatcc gcagagatgc tttctcgtga cagtgaaaat ttcaccatat aagaaaaagt
     7321 cacctgcaaa atggaaaaca atcgcaattt ccctgccaga caatttcatt cgctcacgtt
     7381 ctttgccggt ctttgtattg gtattacgcc tgtggcacag gcactcgctg ccgaagggca
     7441 agctaacgcg gatgacacgc tggttgtcga agcatcaacg ccttcgcttt atgcgccaca
     7501 aaaatctgcc gatccgaaat tctcgcgtcc ggtagcggat actacccgca cgatgacagt
     7561 gatttctgaa caagtgatta aagatcaggg tgcaaccaac cttaccgacg cgctcaaaaa
     7621 cgtccccggc gtgggtgcgt tttttgcggg tgagaacggt aactccacca ctggcgacgc
     7681 catttatatg cgcggtgccg atacctctaa cagtatttat atcgatggca ttcgcgatat
     7741 cggcagcgtc tcgcgcgaca ccttcaatac cgagcaggtc gaagtaatta aagggccgtc
     7801 cggcaccgac tacgggcgca gcgcaccgac aggctcgatc aatatgatca gcaaacagcc
     7861 gcgcaatgat tccggcattg acgcctccgc cagtattggc agcgcctggt tccgccgcgg
     7921 cacgctggac gtcaatcagg tcattggtga taccactgcg gtgcgcctga atgtaatggg
     7981 cgaaaaaacg cacgatgccg gacgcgacaa agtcaaaaat gagcgttacg gcgtcgcccc
     8041 ttctgtcgct tttggccttg gtacagcgaa tcgtttgtat cttaattatc tgcatgtcac
     8101 ccagcacaac acgccagacg gcggcattcc gaccatcggt ttaccgggtt tttctgcccc
     8161 atctgcggga acagcggccc tgaatcattc cggaaaagtt gatacgcata acttttacgg
     8221 cacggattcc gattacgacg attcgaccac cgacaccgct accatgcgtt ttgagcacga
     8281 catcaacgat aacaccacca ttcgcaacac tacgcgttgg tcgcgcgtaa agcaggatta
     8341 cctgatgacg gcgattatgg gtggagcgtc gaatattacc cagcccacca gcgatgtgaa
     8401 tagctggacg tggtcacgca cggcgaatac caaagatgtg agtaacaaaa ttctcaccaa
     8461 ccagaccaac ctgacctcga cgttctatac cggcgctatc ggtcatgatg tcagtaccgg
     8521 cgtggaattt acccgcgaaa cacagactaa ctatggcgtt aatccggtga cgttaccgac
     8581 ggtaaatatt tatcatcctg acagcagcat tcatcccggc ggcctgacgc gcaacggcgc
     8641 aaacgccaat ggtcagacgg ataccttcgc aatttatgcc ttcgatacgc tgcaaatcac
     8701 ccgtgatttt gagctgaacg gtgggatccg tctggataat tatcatactg aatatgacag
     8761 tgccaccgcc tgcggcggca gcgggcgcgg tgccatcacc tgccctgctg gcgtggcaaa
     8821 aggttctccg gtaaccaccg tcgacaccgc caagtcgggc aatctggtga actggaaagc
     8881 cggggcgctg tatcacctga cggaaaacgg caatgtttat attaactatg ccgtttccca
     8941 gcagccgccg ggcggcaaca acttcgccct tgcgcagtct ggcagcggta acagtgccaa
     9001 ccgcaccgat tttaaaccgc aaaaagccaa caccagcgag attggcacca aatggcaggt
     9061 tctggataaa cgcctgctac ttaccgccgc gctgttccgt actgatattg aaaatgaagt
     9121 tgagcaaaat gatgatggga cttattcgca atatggtaag aaacgtgtcg aaggctatga
     9181 gatatccgtg gccgggaata tcacccccgc gtggcagatg attggcggct atacccagca
     9241 aaaagcaact atcaaaaacg gcaaagatgt tgcccaggat ggttcctcat cgctgccgta
     9301 taccccggaa cacgccttca ccttatggag ccagtatcag gcaaccgatg atatttctgt
     9361 tggtgcgggc gcacggtata tcggcagtat gcataaaggt tcagacggcg cggtgggaac
     9421 gccagcattt accgaaggtt attgggtcgc cgacgccaaa ctggggtatc gggttaatcg
     9481 caatctcgac ttccagctaa acgtctacaa cctgtttgat accgattacg tcgcctcaat
     9541 caataagagc ggctaccgtt atcacccggg cgagccaaga accttcttgc tcacagccaa
     9601 tatgcatttc tgattcagat gtggggcgca ggccccactt tttggagaac ttgtatgatg
     9661 taccacattc ccggcgtgtt atcgccacag gacgtcgccc gttttcgcga acacctggaa
     9721 caagccgaat gggtggatgg acgcgtcacc accggcgcac aaggtgcgca agttaagaac
     9781 aatcaacaag tagacacccg cagcgccctg tacgccgccc tgcaaaatga ggtgctgaac
     9841 gcggttaacc aacatgcttt gttctttgcc gcggccttgc cgcgtaccct ttccacgccg
     9901 ctgtttaatc gctatcagaa caatgaaacc tatggttttc atgtggatgg cgcagtacgc
     9961 agccatccac aaaacggctg gatgcgcact gacctttctg ccacgctgtt tttaagcgat
    10021 ccagaaagct acgacggcgg cgaactggtc gttaatgaca ccttcgggca acatcgggta
    10081 aaactcccgg caggcgatct ggtgttgtat ccctccagca gcctgcattg cgtgacaccc
    10141 gtaacccgcg gcgtgcgagt ggcatcattt atgtggatcc agtcgatgat ccgcgatgat
    10201 aaaaagcgcg ccatgctgtt tgaactggac aacaatattc agtcgctgaa aagccactac
    10261 ggcgaaagtg aagagatcct gtcgctgctc aatctttatc ataatctgct gcgggaatgg
    10321 tcggagatct gacgcttgaa ctgcccgagt tcgtccctat attagctaca cttaactgta
    10381 caagcattga tatggggagg acgatatggc atccggttgg gctaatgatg acgccgtcaa
    10441 cgaacagatc aacagtacaa ttgaagatgc gattgcccgc gctcggggtg aaattccgcg
    10501 tggcgaaagc ctgtatgaat gtgaagagtg cggtgcccct atcccgcagg cccgtcggga
    10561 agccattcct ggcgtgcgct tatgcattca ttgtcagcag gagaaagatt tacaaaaacc
    10621 agcttataca ggatataatc gcagaggttc gaaagacagc cagttacgtt aactacaact
    10681 ggcgggatgt atagcgaata taatttgtat tttgcgtggc attccgtgcc tttaacgcta
    10741 ccgtgactgc cacgcggtat aaaacaagtc cataagtaca acaaataaat ggtttatcag
    10801 tatgttagat atcaatcaat ttatttgaac aaggtggtca attctcttcg attttatctc
    10861 tcgtaaaaaa acgtgatact catcacatcg acgaaacaac gtcacttata caaaaatcac
    10921 ctgcgagaga ttaattatga aaactatcaa tactgttgtt gctgctatgg ctctttcaac
    10981 tctgtcattt ggcgtgttcg cggcggaacc ggtaacggca tcccaggcac agaacatgaa
    11041 taaaatcggc gtggtttctg ccgatggcgc atccaccctc gatgccctgg aagcgaaact
    11101 ggctgagaaa gccgcggcgg ccggtgccag tggatacagc atcacttctg ccaccaacaa
    11161 caataaatta agcggtactg cggtaattta taagtaattc gtaagctgga tcaggcgttt
    11221 acgctacgtt cagcacaagt atttccctgt ctgtttgccg acagacgcat atgctctaac
    11281 cctcatgatc ctatgttacc cttgtttgcc cgtccgccac tggacgggct ttttttagct
    11341 ggctagctgc tgacagaaag cctgcaacgt ctcttccggc ataccaatct gccgcgccgc
    11401 atcacaaatg gcctgccagc ttcccgcatc cagtggaatc ccctgctcct gccgttcgcg
    11461 acgcgtgttc acttcccact cgcccggtag caaaatcggc ttatcgtcat catgcggcga
    11521 ggctttcacc cactcggcaa aggcttcggt ctgcgcacta caatccggcg cgccgaacag
    11581 ttccgggttg ataatgatag tggtcatgca gttaagaatg gcatcgggac tggtttgtaa
    11641 cgtttcctga tgcgtcgttt taccgccaga aagcgccccg ccaagaattt cacacatcgc
    11701 tgcaagggcg tagcctttat gttcggcaaa ggtcaacagc gaacccaacg gcgactcctg
    11761 cattaccgcc ggatttgtcg tcggcacgcc gttaacgtca atcaggcaac ctggcggcac
    11821 ggggacgcct ttatgccagg cgacgcgggt tttgccaaat gcaatggcgc tggtggcgta
    11881 atcgagcaac agcgggaaat tatctttacg agggaaaacc acacagaacg gattggtgcc
    11941 aaagcggctg tcgcgaccgt ggaacggcgc gaccattgga ataccgacca cgctaacaaa
    12001 gtggatagag acaaaccccg ccgctgcaca ctgctccgcc cagtaaccga tacggccgat
    12061 atgatgcgag ttatgtagcg ccacggcggc aatgccgtgc tgatgcgctt tctcaatccc
    12121 cagcgccatc gcttcgtgtg ccgcgacctg accaaatgcg cgatcgccat cgagcgtgac
    12181 cgccgccccc gcttctttaa cgactttggc atgatggtta atttgcagat gcccctgact
    12241 ccaggagcgc acatagcttg ggatcatgcc aataccatgt gaatcatgcc ctgccaggtt
    12301 tgccgcgatc aaatgatcgg cgactaattt cgcttcctgt tcctcactac ccatctgacg
    12361 aaatacagcc tgaataaaac tgtgcagcgt ctgagcatca aagcgatggc cactttccat
    12421 ggttaactcc tgtttatgtt atgtgtttgt tgttttttat gctgcacgcc gggcgcggga
    12481 aggtcaataa agatgcagga atttcatatt gcggtggggt aaaagccgga tgacatggct
    12541 catccggctg agataaaaat taaaatgcct gattaacgcg cgccaggccg tcgctgatag
    12601 ttgccgcttc acccgtagcc atcaggcagc aagccatctg gattttcagc gattcgggaa
    12661 tcggttcgct gccagccagg caacgctcaa tccactgcgc cgtggtttcc ggatcttttg
    12721 cttgtggcag taactcgcta ccagcagtgt cctgtttttc atacagtacc cgcatccctt
    12781 cacggtcaat gagattgatc tgcgggcagc gctgcggatt agcatacact tcaccttcag
    12841 taccatgcat aagtagcgcc cgcccgccga tatcgctaaa gaacttcgcg acgcgtccaa
    12901 tgtattccgg atgcgaaacg ctggaaagac gcagtgcctc accttcggca aatggcgtcg
    12961 ccagtttcgc cagggtatgt gcactgttac gcacgcccat ccgccagcgc atcgccagtt
    13021 gtttttccag cggcgggcaa aacgcgccga caggcataaa gacaggttga tgttcgtcga
    13081 gcttcgcctg cgcctgcccg ccgtgcaacg ttggcgtaat acccatcagt tcgaaaatgg
    13141 tttcggtcag cacgcgggtt ggatcttcgc taaccccgtg aaccaccaca ggaaaaccga
    13201 gtttgtgcag cagaatcgcc agcaacggcg tcaggttggc ctgtttacgc gcgccgttgt
    13261 aactggggat gacaatcggc atcggcttgc ctgcaggcgg cgtcagcttg atggtgtgat
    13321 tttgcatggc ttcgtaaaag ccgagcatct ctgcttcccc ttcccctttg atacgtagcg
    13381 caatcaatac gccgcccaac tcaaggtcag ggacttcacc attgagcata tgggcataca
    13441 gaccgcgcgc agtatcccgg tctaaatcgc gcgcgtggtt tttcccgcgc ccgatctctt
    13501 taatgatttt gcgatagtcc atttacgact ccttacctga ctcacatcat taacgccgcc
    13561 gacggcgtgg ggatttcgtt ttttctttct ttttaactat accttcaggg acttccggtt
    13621 gctctatcgg aaataccggt aatgcatcca gtagtcgctt gccataattt tttgtcagca
    13681 agcgtttgtc gtagataacc acttcccccc agcaaccgtg gcttcgaatc agtcgcccaa
    13741 cctgctgaat caggttaaac gaagcgctcg gcaggctttg cacctcaaac ggatagcggt
    13801 taaggctttt cagccattca ccttcggtga tcaccaccgg actgtcgatg ggcggaaaag
    13861 cgattttgtg gatatgcacc tggctgagca gagcgccttt cagatcaaga ccttcggcaa
    13921 aggactgtaa gcccaccagc acgctacgct caccgttggc aacgcgtttg cggtgcagtt
    13981 caactaagcg ataacgtggc tgatcgccct gcaccagcaa catcaggcgt aaatccgtca
    14041 catagtcgag aaagcgctgc atcgcacgcc cgctggcaaa cagcaccaac ataccgagat
    14101 gttttttgct ctccacctgc tcacggaaaa aggccgccat ttcagcaatg tgctgttctt
    14161 cgttgtcgat ggaaggctca acgcgcatcc ggggaataac aattttgccc tgttcgcagt
    14221 ggttaaaggg cgaatccagc gccacaaagc ggtcacccgc tttctctttc agcccactca
    14281 tctcctgcaa acgcgaaaaa ctgttcagcg aacgcaaggt tgcggaggta acaataatgt
    14341 gcggaatact gcgccacagc agcctttcca gttggtcgct aacacgaata cccacacagt
    14401 gaaaccacag atgtagctgc ccttcgcgct cttcccgcgt cgcccatttg gtcaccggcg
    14461 caccggaaga ttgcgccaga gacgccagcc gccagagttt gctttgcgcc tcgaacatcc
    14521 ccaacgcgcg gttcatctgc aaaatcaacc gatgcaggcg tacaatgtca tggctgccgg
    14581 ttttctcact taaatcgtta agaaataact ccgccagacc acgcagcatc tcggtgagtt
    14641 ttgccagccg ctggcagatc tccagcactt catcaggcag ttcgcccatc gcaaaacggt
    14701 gctctgcctc ctgcccggca ggcatgtaga gattgagaat gttgtttaac gaggcgataa
    14761 gctcatacag ctcttcacaa tgcgcattca aacgttcagg gatcgccagc ggtgggatgg
    14821 tcttcgggcg aaactgctcc atgcaggtgg cgaccagttt ggtgaacagg tccagttgga
    14881 gccggtacca gggcgcagtg atttcggcgc tcatctccag cgcatcccgc gccacatccg
    14941 gcaggtgatg accttcgtcc agcaccagca gtaaattttt cgggtcaggc aataccgctt
    15001 cgctttccat tgccgccatc accagcgcat ggtttgccac caccacttcc gcttcctgaa
    15061 tttcccgacg agcgacaaaa aacgggcatt cgcgatagta gtaacagttg cggttgaggc
    15121 agctggcttt gtcggtactc aaacgacgcc agagatcatc atctatagcg atatcagtat
    15181 gatcgcgcag gccatcccat ttataagtgt cgagatcgcc cttcagcttc gcacaacgtt
    15241 tttgctcttc ctgattgttc ggcgtcagtt cgtcgtcaag aaacgccagc agatcctgtt
    15301 gcgtgggctc agtgctggcg agcgctgtca gattacgcgg gcaaacgtag cgcccacgcc
    15361 caaaagcggc agtgaattta agatcgggaa tgatcttttt cagcagcggt aaatctttgc
    15421 tgtagatctg atcctgcaat gccacgttgg cggtacttac caccagcgtt ttttgctctt
    15481 cgcgggcaat ggcgatgccg gggatcaaat aggagagcgt tttcccaacg ccggtaggag
    15541 cttcaatcgc cagatgccgc ccttcttctc cggccagcgt tttggcgacg tccgcaatca
    15601 tctgccgctg cggcgcacgg ggaataaagt cggggatctg ttcctgaagc gccttatacc
    15661 aggcggcaat ttgcgcttta agcgcggcgg ttaatgccat gagaaaacct gaaatactgt
    15721 ataaacagcc aatattgtgg catttttcgg ttttcagggg taatggtttt tctgtttctg
    15781 gaaagcggct cgcaataacc agcaggatga tgcgtaaagc gcttcgcgct aaattcgaac
    15841 aacatgcaga actgcgtaca ctcttgctgg caaccgcgtc cgcaaaactg gttgagcata
    15901 cgcaaaatga tgcttactgg ggagatggcg gtaatggtca gggcaagaat cgcctggggt
    15961 accttttaat ggcgttgcgc ggacaattgg ctgcggagaa gtaacgcatc gggtggcttg
    16021 tgggcgttaa aacagagaaa tctttttgcc gcgctcgcaa atggctgaca agtgcttgtt
    16081 gtgaaatgcc ggatgcggcg cgagcgcctt atccggccta caaaaacatg cacattcaat
    16141 atattgcaga gttcacgtag gcctgataag cgtagcgtat caggcagttt tgtgtttgtc
    16201 atcagtctca tgccgggcat agcccggcat aaaagattac tgcgcagcgg caggtttacg
    16261 cggacggcgg cgacgttgtt gttcgcctgc cggtttggca tcgccgagac gacgagacgg
    16321 tttttctgcg ggcttcgcgt taccagactt cgcaccactt tccgcacggc gtggttgctg
    16381 ttgaccgcga ccaccgccct gtccacgacc gccgccacgt tgctggcgac cgttttggat
    16441 cggttcggct ttgattgacg ggtccggctc atagcccaga atcgcaatgc gcgggatctc
    16501 ttttttcagc agtttttcga tatcacgcag cagtttgtgt tcatcaacac acaccagcga
    16561 caatgcttcg ccagttgccg ccgcacggcc agtacgcccg atacggtgaa cataatcttc
    16621 cggtacgttt ggtaactcat agttgacgac gtgcggcaac tcttcgatat ccagaccacg
    16681 cgcagcgatg tcagttgcca ccagtacacg aatatcgccc gatttaaaat cggccagcgc
    16741 acgagtacgc gcaccttgcg atttattacc gtggatcgcc gcactacgga tgccatcttt
    16801 attaagctgt tcagccagat ggttagcgcc atgtttggta cgggtaaaca ccagcacctg
    16861 ctgccagttc cctttcccaa tcatatgcga cagcaattcg cgtttgcgtt tcttatcgac
    16921 aaagtgaacg tgctgggtca cctgatcaga cgcagtattg cggcgtgcca cttcgatttc
    16981 cagcgggttg tgcaacagtt tttccgccag ggctttaata tcgtcagaga aggtcgcgga
    17041 gaacaacagg ttctggcgct tcgctggcag ttttgttaac acgcgacgga tatcgtggat
    17101 aaagcccatg tcgagcatgc ggtcagcttc atcaaggacg aggatttcaa cctgatccag
    17161 cttcactgca ttttgatgtt ccaggtccag cagacgtccc ggcgttgcca ccagcacatc
    17221 aacgccgccg cgcagcttca tcatctgcgg gttaatgctg acaccgccaa acaccaccag
    17281 cgaacgaatg ttcaggtatt tgctgtaatc acggacgttt tcgccaatct gcgccgccag
    17341 ttcacgggtc ggggtaagaa tgagcgcacg caccggacga cgccctttgg cgtgcggctg
    17401 gcgagtgatc aggtgttgca acagcggcag cgtaaagccc gctgttttac ctgtaccggt
    17461 ctgggcgctg gccatcaggt cgcggccttc cagcaccgca gggatcgcct gctgctgaat
    17521 aggggtgggt tcacggtaac cctgctcggc aacggcgcgc aggatatcag ggcttaaacc
    17581 caaagaatcg aaagacataa ctactccgaa ccgccccgac cgttaccggt gtagttttca
    17641 gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc atgagggagc ggagtgtagc
    17701 agtttttgtg acgcagcgca taaattatcc ctgtgactgg cgtagctaaa attcttagcc
    17761 atactggaca actcccctat ggagtcataa tcttaatcaa tcatttgatt aagaatggac
    17821 tgtgcgatga ataatcctgc catgacaatc aagggtgaac aggcgaaaaa acagctgatt
    17881 gctgcggcac tggcacagtt tggtgaatat ggaatgaacg ccaccacccg cgagatagcc
    17941 gcccaggccg ggcagaatat cgctgccatc acctactact tcggttcgaa agaagatttg
    18001 tacctcgcct gcgcccagtg gattgctgat tttattggcg agcagttccg tccgcacgcc
    18061 gaagaagccg aacgtttgtt cgcgcaacca cagccggatc gggctgccat ccgtgaactg
    18121 atccttcgcg cctgcagaaa catgattaag ctgctcaccc aggatgacac cgtcaacctc
    18181 agcaaattta tctcccgtga gcagctctct cccacggcag cctaccacct ggtgcacgaa
    18241 caggtgatta gtccgctaca cagtcacctt acacgcctga ttgccgcctg gaccggctgc
    18301 gacgctagcg atacccgcat gatccttcat acccatgcgt tgattggtga gattctggcg
    18361 tttcgtcttg gcaaagaaac tattctgtta cgtaccggct ggaccgcgtt cgatgaagaa
    18421 aagaccgaac tgatcaacca gacggtgacc tgtcatattg acctgatttt gcaaggatta
    18481 tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat cggactggcg gtagtagtac
    18541 ttgccgccgt ggttgccgga ggctactggt ggtatcaaag ccgccaggat aacggcctga
    18601 cgctgtatgg caacgtggat attcgtacgg tgaatcttag tttccgtgtt ggggggcgcg
    18661 ttgagtcgct ggcggtggac gaaggtgatg cgatcaaagc gggccaggtg ctgggcgaac
    18721 tggatcacaa gccgtatgag attgccctga tgcaggcgaa agcgggtgtt tcggtggctc
    18781 aggcgcagta tgacctgatg cttgccgggt atcgagatga agaaatcgct caggccgccg
    18841 cagcggtgaa acaggcgcaa gctgcctatg actatgcgca gaacttctat aaccgccagc
    18901 aaggactgtg gaaaagccgc accatttcgg caaatgacct agaaaatgcc cgctcctcgc
    18961 gcgaccaggc gcaggccacg ctgaaatcag cgcaggataa attgcgtcag taccgttccg
    19021 gtaaccgcga acaggacatc gcccaggcga aagccagcct cgaacaagca caggcgcaac
    19081 tggcgcaggc ggagctgaat ttgcaggact caacgttgat agcaccgtct gatggcacgc
    19141 tgttaacgcg cgctgtagag ccaggcacgg tgcttaatga aggtggcacg gtatttaccg
    19201 tctcgctgac gcgtccggtg tgggtacgcg cctatgttga tgaacgtaat cttgaccagg
    19261 cccagccggg gcgcaaagtg ttgctttata ccgatggtcg cccggacaag ccgtatcacg
    19321 ggcagattgg tttcgtttcg ccgactgctg aatttacccc gaaaaccgtc gaaacaccgg
    19381 atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt gaccgacgcc gatgatgcgt
    19441 tacgccaggg aatgccggta acggtgcaat tcggtgacga ggcaggacat gaatgatgcc
    19501 gttatcacgc tgaacggcct ggaaaaacgc tttccgggca tggacaagcc cgccgtcgca
    19561 ccgctcgact gcaccattca cgccggttat gtgacggggc tggttgggcc ggacggtgcg
    19621 ggcaaaacca cgctgatgcg gatgctggcg ggattactga aacccgacag cggcagtgcc
    19681 acggtgattg gctttgatcc gatcaaaaac gacggcgcgc tgcacgccgt gctcggctat
    19741 atgccgcaga aattcggcct gtatgaagat ctgacggtga tggagaacct caatctgtac
    19801 gcggatttgc gcagcgtcac cggcgaggca cggaagcaaa cttttgctcg cctgctggag
    19861 tttacgtctc ttggcccgtt taccggacgc ctggcgggca agctctccgg tgggatgaaa
    19921 caaaaacttg gcctggcctg taccctggtg ggcgaaccga aagtgttgct gctcgatgaa
    19981 cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt ggcagatggt gcatgagctg
    20041 gcgggcgaag ggatgttaat cctctggagt acctcgtatc tcgacgaagc cgagcagtgc
    20101 cgtgacgtgt tactgatgaa cgaaggcgag ctgctgtatc agggagaacc gacggccctg
    20161 actcaaacca tggccggacg cagctttctg atgaccagcc cgcacgaggg caaccgcaaa
    20221 ctgttgcaac gggcattgaa actgccgcag gtcagcgacg gcatgattca ggggaaatcg
    20281 gtacgtctga tcctcaaaaa agagaccaca ccagacgata ttcgccatgc cgacgggatg
    20341 ccggaaatca acattaacga aactacgcca cgttttgaag atgcgtttat tgatttgctg
    20401 ggcggtgccg gaacctcgga atcgccgctg ggcgcaatat tgcatacggt ggaaggtaca
    20461 cctggcgaga cggtgatcga agcgaaagag ctgaccaaaa agtttggcga ttttgccgcc
    20521 accgatcacg tcaactttgc cgtcaaacgc ggtgaaatct ttggtttgct cgggccaaac
    20581 ggcgcaggga aatcgaccac ctttaagatg atgtgcggtt tgctggtacc gacctctggt
    20641 caggcgctgg tgctgggcat ggatctgaaa gagagttccg gtaaagcgcg ccagcatctc
    20701 ggctatatgg cgcaaaaatt ttcgctctac ggcaacctga cggtcgaaca gaatttacgc
    20761 tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat ctcccgtatg
    20821 agcgaggcgt tcggcctgaa aagtatcgcc tcccacgcga ccgatgaact gccattaggt
    20881 tttaaacagc ggctggcgct ggcctgttcg ctgatgcatg aaccggacat tctgtttctc
    20941 gacgaaccga cgtccggcgt tgatcccctc acccgccgtg aattttggct acatatcaac
    21001 agtatggtag agaaaggcgt cacggtgatg gtcaccaccc actttatgga tgaagcggaa
    21061 tattgcgacc gcatcggcct ggtgtaccgc gggaaattaa tcgccagcgg cacaccggac
    21121 gatttgaaag cgcagtcggc caacgatgag caacccgatc ccacgatgga gcaagccttt
    21181 attcagttga tccacgactg ggataaggag catagcaatg agtaatccga tcctgtcctg
    21241 gcgtcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg atccgagtag
    21301 ctggctgatt gcagtagtga tcccactgct actgctgttt atttttggat acggcattaa
    21361 cctcgactcc agcaagctgc gcgtcgggat tttactggaa caacgtagcg aagcggcgct
    21421 ggatttcacc cacaccatga ccggttcgcc ctacatcgac gccaccatca gcgataaccg
    21481 tcaggaactg atcgccaaaa tgcaggcggg gaaaattcgc ggtctggtgg ttattccggt
    21541 ggatttcgcc gaacagatgg agcgcgccaa cgccatcgca ccgattcagg tgattaccga
    21601 cggcagtgag ccgaataccg ctaactttgt gcaggggtat gtcgaaggga tctggcagat
    21661 ctggcaaatg cagcgagcgg aggataacgg gcagactttt gaaccgctta ttgatgtaca
    21721 aacccgctac tggtttaacc cggcggcgat aagccagcac tttattatcc ccggtgcggt
    21781 gactattatc atgacggtca tcggtgcgat tctcacctcg ctggtggtgg cgcgagaatg
    21841 ggaacgcggc accatggagg ctttgctctc tacggagatt acccgcacgg agctgctgct
    21901 gtgtaagctg atcccttatt actttctcgg gatgctggcg atgttgctgt gtatgctggt
    21961 gtcggtgttt attctcggcg tgccgtatcg cgggtcgctg ctgattctgt tttttatctc
    22021 cagcctgttt ttactcagta ccctggggat ggggctgctg atttccacga ttacccgcaa
    22081 ccagttcaac gccgctcagg tggccctgaa cgcggctttt ctgccgtcga ttatgctttc
    22141 cggctttata tttcagatag acagtatgcc cgcggtgatc cgcgcggtga cgtacattat
    22201 tcccgcacgt tatttcgtca gcactctgca aagcctgttc ctcgccggga atattccagt
    22261 ggtgctgata gtgaacgtgc tgtttttgat cgcttcggcg gtgatgttta tcggcctgac
    22321 gtggctgaaa accaaacgtc ggctggatta gggagaagag catgtttcat cgcttatgga
    22381 cgttaatccg caaagagttg cagtcgctgc tgcgcgaacc gcaaacccgc gcaattctga
    22441 ttttacccgt gctgattcag gtgatcctgt tcccgttcgc cgccacgctg gaagtgacca
    22501 acgccaccat cgccatctac gatgaagata acggcgagca ctcagtagaa cttactcaac
    22561 gttttgcccg cgccagcgcc tttacccatg tgctgctgct gaaaagcccg caggagatcc
    22621 gcccgaccat cgacacacaa aaggcgttac tactggtgcg tttcccggct gacttctcgc
    22681 gcaaactgga taccttccag accgcgccgt tgcagttgat cctcgacggg cgtaactcca
    22741 acagtgcgca aattgccgcc aactacctgc aacagatcgt caaaaattat cagcaggagc
    22801 tgctggaagg aaaaccgaaa cccaacaaca gcgagctggt ggtacgcaac tggtataacc
    22861 cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc accactatcg
    22921 gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg tgaacaaggt acgctcgatc
    22981 agttgctggt ttcgcccctc accacctggc agatattcat cggcaaagcc gtaccggcat
    23041 taattgtcgc caccttccag gccaccattg tgctggcgat tggtatctgg gcgtatcaaa
    23101 tccccttcgc cggatcgctg gcgctgttct actttacgat ggtgatttac ggtttatcgc
    23161 tggtgggatt cggtctgttg atttcatcac tctgttcaac acaacagcag gcgtttatcg
    23221 gcgtgtttgt ctttatgatg cccgccattc tcctttccgg ttacgtttcg ccggtggaaa
    23281 acatgccagt gtggctgcaa aatctgacgt ggattaaccc tattcgccac tttacggaca
    23341 ttaccaagca gatttatttg aaggatgcaa gtctggatat tgtgtggaat agtttgtggc
    23401 cgctactggt gataacggcc acgacagggt cagcggcgta cgcgatgttt agacgtaagg
    23461 tgatgtaact tcttatcttt cgccagcaaa gacaccaccg ccgggccggc aaggattgcc
    23521 agccctgcaa ttgccagcaa aaagttgttt tccagtacgc gggacaacag ccacagcaca
    23581 atcagcgctg caccaaaata ccaggtcgtg gtcagctctt ccagcacgtc tgccacctca
    23641 cgccagcgtt gctctgggtg gcgctgaaac accagcatca tcactaccgt gacacaatac
    23701 agtgcgcata atcctacgcc gacgtgaacc agagttttac tcatccaacc caccaccagc
    23761 agcgccacta ccagtaaatg caacataatc tgccagcaac ttaggccagt tgcgacacga
    23821 acacgttgtt gccacttcat ggcttctctc ctgaagaatt tatctctact tattcagagt
    23881 accgaacttt acggaaaatt ccgtaagacc gcaccttttt gtgacgccga ctacactatt
    23941 tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa caattttcgt
    24001 tcaaggtgct caccatcaat attcacaaag gctttaccgc gtttaaccga cgcttcattt
    24061 taccggaact tcgcgacgcc gtgcgtaccg tcagcgccga tattgtttgc ctgcaggaag
    24121 tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa ctggcccgat acctcgcact
    24181 acgagtttct cgccgacacc atgtggagcg atttcgccta cggtcgcaat gccgtatacc
    24241 cggaaggaca tcacggtaac gccgttcttt cacgttatcc cattgaacat tatgagaatc
    24301 gtgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc attgtgccgc
    24361 cgatgaccgg gaaagcgatt catgtgatgt gcgtacatct gggcctacgt gaggcgcacc
    24421 gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga gctaccggac ggcgaatcgg
    24481 tattggtagc gggtgatttc aacgactggc ggcaaaaagc taatcatccg ttaaaagtgc
    24541 tggccgggct ggatgagatt tttacccgcg cccacggacg tccggcgcgc acgtttccgg
    24601 tgcaatttcc tctactacga ctggacagga tctacgtcaa aaatgccagc gccagcgcgc
    24661 caaccgcgtt gccgctgcgg acatggcgac acctttctga tcatgcccct ttaagtgcgg
    24721 agattcattt atgaaatgta gctggcgcga aggcaatatg atccagttgc tggaaaacgg
    24781 cgagcaatat tatcccgcgg tgtttaaggc gattggcgag gcgcaagaac gcatcattct
    24841 tgaaacgttt atctggtttg aggatgacgt cggcaaacag ctgcatgcgg cgctattggc
    24901 agcagcgcaa cgcggggtaa aagcggaagt cttgctggat ggctacggtt cgccggatct
    24961 cagcgatgaa tttgtcaatg aactgacggc agctggcgtg gtgttccgct actacgatcc
    25021 ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc gcaaaattgt
    25081 ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat tactccgccg agcatatgtc
    25141 cagctacggc ccggaggcca aacaggatta cgcggtacgc ctcgaagggc cgattgtcga
    25201 agatattctc cagtttgagc tggaaaacct gccaggacaa agcacggcgc gacgctggtg
    25261 gcgacgccat cataaagcgg aagagaaccg ccagccggga gaagcgcagg tattgctggt
    25321 ctggcgcgat aacgaagaac atcgcgatga tatcgaacgt cactatctga aaatgctcac
    25381 tcaggcgcgg cgggaagtga ttatcgccaa cgcctacttc ttccccggct atcgtttttt
    25441 acacgccttg cgtaaagcgg cacgacgcgg ggtgcggatc aaactgatca ttcagggcga
    25501 accggatatg ccgattgtca gagtcggcgc gcgtttgctg tataactatc tggttaaagg
    25561 tggcgttcag gtgtttgagt accgccgccg cccgctccac ggcaaagtgg cattgatgga
    25621 cgatcactgg gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt cactgaatct
    25681 cgaagcaaat gtcatcatcc acgatcgtaa ttttaaccag acgctgcgcg ataatctgaa
    25741 cggcattatc gccgcagatt gtcagcaggt cgatgagacc atgctgccga aacgcacctg
    25801 gtggaacctg actaaaagcg tgctggcgtt ccactttcta cgccacttcc cggcgctggt
    25861 gggctggctt ccggcgcaca cgccacgtct ggcgcaggtt gatccccccg cacaaccgac
    25921 aatggaaacg caggatcggg tagaaactga aaacacgggg gtaaaaccct gatgagtaaa
    25981 tcacacccgc gctggcgctt agcaaagaag atcctcacct ggctgttttt tatcgcggtg
    26041 attgtgttgt tggtggtcta cgccaaaaaa gtggactggg aagaggtctg gaaggtcatc
    26101 cgagactaca atcgcgttgc actgctcagt gcggtcgggc tggtggtcgt cagctatctg
    26161 atttacggtt gctacgacct gctcgcccgt ttctactgcg gtcacaaact ggcgaagcgc
    26221 caggtgatgc tggtgtcgtt tatctgctac gccttcaacc tgacactcag tacctgggtc
    26281 ggcggcattg gtatgcgcta tcgtttgtac tcccgactgg ggttaccggg cagcactatt
    26341 acgcggattt tttcgctcag tattaccacc aactggctag gctatatttt gctagcgggg
    26401 gttatcttta ccgcaggcgt ggtggagttg ccggatcact ggtatgtcga tcaaactacg
    26461 ctgcgtattc tcggcattgg cttactgatg attatcgcgg tttatttgtg gttttgcgct
    26521 ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc tggtgctgcc ttcgtggaaa
    26581 ttcgccctcg cccagatgct gatttccagc gttaactgga tggtaatggg agcgattatc
    26641 tggctgttac ttggtcaaag cgtaagctat ttctttgtac tgggcgtgtt gctggttagt
    26701 agtattgctg gcgtcatcgt gcatattccg gcgggaattg gtgtgctgga agcggtgttt
    26761 atcgcgctac tggctgggga gcatacctcc aaaggcacaa ttatcgccgc cctacttgct
    26821 taccgtgtgc tgtattactt tatcccactg ctgctggcgc tgatctgtta cctgttgctg
    26881 gaaagccagg cgaagaagct acgggcgaaa aatgaagcgg cgatgtgaag tatagttaag
    26941 ttctttagta tgtgcattta cggctaatga aaaaaccgcg tatgcctttg ccagacaagc
    27001 gttatagcta tagcagcaag aatcccaaag aaaccgataa aatagtaata gagtgttaat
    27061 gcacctataa aagcacaacg aaccggactc cataattctt cactatggct ttttcctgtc
    27121 agttcaagga gcttatttcg tccatgcgag ataatgcctg accataagac aatcattaag
    27181 caacttatta cccaaatggg tcgttcactc attaatgtgg cattcacgat gattaccagg
    27241 gccaaccctg tcaacgtcat cataatgata aaacgatgag aaccaggatc aaatttaaac
    27301 aatagacctg ccagcatact gattaaaaac atcgatcccg cagtgctgaa cacgacgata
    27361 atggcagata caggcgctaa actcgcaacg acaatcgcca ccaaagagat taaaaacaaa
    27421 tatattacgc ccgcgatggc ggtatttatc ttgttgatat ctttacttaa gccatacata
    27481 gaaagtaata ataatcctat agcaacatac atttgcggtg aatcatattt tgttgccgta
    27541 atttcgagat acttagcgcc ccaggccagc cctgcggttg ccaccaaacc caaagcggta
    27601 atgagccata tgccgtttaa tatcctggct tcgtgctgaa aatctaattt attgctattt
    27661 tgcgagtatg attccattct tttagttgtc cttagaaaaa acattaatat tcaccatcaa
    27721 taaatt
//
LOCUS       DACADA010000054        26985 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.055, whole genome shotgun sequence.
ACCESSION   DACADA010000054 DACADA010000000
VERSION     DACADA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.055"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            50..2230
                     /gene="katG"
                     /locus_tag="ISB17_002215"
     CDS             50..2230
                     /gene="katG"
                     /locus_tag="ISB17_002215"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAP1980147.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2324..3229
                     /gene="yijE"
                     /locus_tag="ISB17_002216"
     CDS             2324..3229
                     /gene="yijE"
                     /locus_tag="ISB17_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAP1980148.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3256..3873)
                     /locus_tag="ISB17_002217"
     CDS             complement(3256..3873)
                     /locus_tag="ISB17_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAP1980149.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(4149..5252)
                     /gene="gldA"
                     /locus_tag="ISB17_002218"
     CDS             complement(4149..5252)
                     /gene="gldA"
                     /locus_tag="ISB17_002218"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAP1980150.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5263..5925)
                     /gene="fsa"
                     /locus_tag="ISB17_002219"
     CDS             complement(5263..5925)
                     /gene="fsa"
                     /locus_tag="ISB17_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP1980151.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(5937..8438)
                     /gene="ptsP"
                     /locus_tag="ISB17_002220"
     CDS             complement(5937..8438)
                     /gene="ptsP"
                     /locus_tag="ISB17_002220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1980152.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8747..9826
                     /locus_tag="ISB17_002221"
     CDS             8747..9826
                     /locus_tag="ISB17_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAP1980153.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            9841..10161
                     /locus_tag="ISB17_002222"
     CDS             9841..10161
                     /locus_tag="ISB17_002222"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP1980154.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10212..12509
                     /locus_tag="ISB17_002223"
     CDS             10212..12509
                     /locus_tag="ISB17_002223"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP1980155.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12475..13353
                     /locus_tag="ISB17_002224"
     CDS             12475..13353
                     /locus_tag="ISB17_002224"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAP1980156.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            13355..13696
                     /locus_tag="ISB17_002225"
     CDS             13355..13696
                     /locus_tag="ISB17_002225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP1980157.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13683..14534)
                     /locus_tag="ISB17_002226"
     CDS             complement(13683..14534)
                     /locus_tag="ISB17_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1980158.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14760..16493)
                     /gene="cptA"
                     /locus_tag="ISB17_002227"
     CDS             complement(14760..16493)
                     /gene="cptA"
                     /locus_tag="ISB17_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAP1980159.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16676..19327)
                     /gene="ppc"
                     /locus_tag="ISB17_002228"
     CDS             complement(16676..19327)
                     /gene="ppc"
                     /locus_tag="ISB17_002228"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAP1980160.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19629..20780)
                     /gene="argE"
                     /locus_tag="ISB17_002229"
     CDS             complement(19629..20780)
                     /gene="argE"
                     /locus_tag="ISB17_002229"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAP1980161.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            20934..21938
                     /gene="argC"
                     /locus_tag="ISB17_002230"
     CDS             20934..21938
                     /gene="argC"
                     /locus_tag="ISB17_002230"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAP1980162.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            21946..22722
                     /gene="argB"
                     /locus_tag="ISB17_002231"
     CDS             21946..22722
                     /gene="argB"
                     /locus_tag="ISB17_002231"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAP1980163.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            22783..24156
                     /gene="argH"
                     /locus_tag="ISB17_002232"
     CDS             22783..24156
                     /gene="argH"
                     /locus_tag="ISB17_002232"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAP1980164.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            24423..25340
                     /gene="oxyR"
                     /locus_tag="ISB17_002233"
     CDS             24423..25340
                     /gene="oxyR"
                     /locus_tag="ISB17_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAP1980165.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(25323..26723)
                     /gene="sthA"
                     /locus_tag="ISB17_002234"
     CDS             complement(25323..26723)
                     /gene="sthA"
                     /locus_tag="ISB17_002234"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAP1980166.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         6109 a         6848 c         7522 g         6506 t
ORIGIN      
        1 ctctaacgct gtgtatcgta acggtaacac tgtagagggg agcacattga tgagcacgtc
       61 agacgatatc cataacacga cagccaccgg caaatgcccg ttccatcagg gcggtcacga
      121 ccagagcgca ggtgggggca caaccacgcg cgactggtgg ccaaatcaac tccgtgttga
      181 cctgttaaac caacattcta atcgttctaa cccgctgggt gaggactttg actaccgcaa
      241 agaattcagc aaattagatt actacggcct gaaaaaagat ctgaaagccc tgctgacaga
      301 atctcagccg tggtggcctg ccgactgggg cagctacgct ggtctgttta ttcgtatggc
      361 ctggcacggc gcgggtactt accgttcaat cgacggtcgc ggcggcgcgg gtcgtggtca
      421 gcaacgcttt gcaccgctga actcctggcc ggataacgta agcctcgata aagcgcgtcg
      481 cctgttgtgg ccaatcaaac agaaatatgg tcagaaaatc tcctgggccg acctgtttat
      541 cctcgcgggt aacgtggcgc tggaaaactc cggcttccgt accttcggtt ttggtgccgg
      601 tcgtgaagac gtctgggaac cggatctgga tgttaactgg ggtgatgaaa aagcctggct
      661 gactcaccgt catccggaag cgctggcgaa agcaccgctg ggtgcaaccg agatgggcct
      721 gatttacgtt aacccggaag gcccggatca cagcggcgaa ccactttcgg cagcagcagc
      781 tatccgcgcg accttcggca acatgggcat gaatgacgaa gaaaccgtgg cgctgattgc
      841 gggtggtcat acgctgggta aaacccacgg tgccggtccg acatcaaatg taggtcctga
      901 tccagaagct gcaccgattg aagaacaagg tttaggctgg gcgagcactt acggcagcgg
      961 cgttggcgca gatgccatta cctctggtct ggaagtggtc tggacccaga cgccgaccca
     1021 gtggagcaac tatttcttcg agaacctgtt caagtatgag tgggtacaga cccgcagccc
     1081 ggctggcgca atccagtttg aagcggtaga cgcaccggaa attatcccgg atccgtttga
     1141 tccgtcgaag aaacgtaaac cgacgatgct ggtgaccgac ctgacgctgc gttttgatcc
     1201 tgagttcgag aagatctctc gtcgtttcct caacgatccg caggcgttca acgaagcctt
     1261 tgctcgtgcc tggttcaaac tgacgcacag ggatatgggg ccgaaatctc gttacatcgg
     1321 gccggaagta ccgaaagaag atctgatctg gcaagatccg ctgccgcagc cgatctacaa
     1381 cccgaccgag caggacatta tcgatctgaa attcgcgatt gcggattctg gtctgtctgt
     1441 tagtgagctg gtatcggtgg cctgggcgtc tgcttctacc ttccgtggtg gcgacaaacg
     1501 tggtggtgcc aacggtgcgc gtctggcatt aatgccgcag cgtgactggg atgtgaacgc
     1561 cgcagccgtt cgtgctctgc cagttctgga gaaaattcag aaagagtctg gcaaagcttc
     1621 gctggcggat atcatcgtgc tggcaggtgt ggttggcgtt gagaaagccg caagcgccgc
     1681 aggtttgagc attcatgtac cgtttgcgcc gggtcgcgtt gatgcgcgtc aggatcagac
     1741 tgacattgag atgttcgaac tgctggaacc aattgctgac ggtttccgta actatcgcgc
     1801 tcgtctggac gtttccacca ccgagtcact gttgattgat aaagcacagc aactgacgct
     1861 gaccgcgccg gaaatgactg cgctggtggg cgggatgcgc gtactgggtg ccaacttcga
     1921 tggcagcaaa aacggcgtct tcactgaccg cgttggtgta ttgagcaatg acttcttcgt
     1981 gaacttgctg gatatgcgtt acgagtggaa agcgaccgac gaatcgaaag aactgttcga
     2041 aggccgtgac cgcgaaactg gcgaagtgaa atacacagcc agccgtgcgg atctggtctt
     2101 tggttctaac tctgtcctgc gtgcggtggc ggaagtctac gccagcagcg atgcccacga
     2161 gaagtttgtt aaagacttcg tggcagcgtg ggtgaaagtg atgaacctcg accgtttcga
     2221 cctgctgtaa tctgaccttc ttcagcgact gcctttcatg cagtcgctga agtttcttta
     2281 ccggcgtata gtgtccacag gaaaactaca cactggatct ctcatgtctg ccgcaggaaa
     2341 gagcaaccca ctggcaatca gtggcctggt tgtgctcaca cttatctgga gttatagctg
     2401 gattttcatg aagcaagtca ccagctacat cggtgccttc gacttcaccg ccttacgctg
     2461 cattttcggc gctctcgttt tattcatcgt tcttttatta cgtggtcgcg gaatgcgccc
     2521 gacgccgttt aaatacacct tagccattgc cctgttacaa acctgcggga tggttggcct
     2581 ggcacagtgg gcgctggtca gcggcggagc ggggaaggtg gcaatcctga gctataccat
     2641 gccgttctgg gtggtgattt tcgccgcgtt gtttctcggt gaacgcctgc gacgtgggca
     2701 atatttcgcg atactgattg ccgctttcgg cttatttttg gtgttgcagc cgtggcaact
     2761 cgatttctct tcgatgaaaa gtgccatgct ggcaatcctc tccggtgtga gttggggggc
     2821 gagcgcgatt gttgctaaac gtctgtatgc ccgccatccg cgcgtggatt tattgtcgct
     2881 aacatcctgg cagatgctgt atgcggcgct ggtgatgagc gtggtcgctt tactggtgcc
     2941 gcaacgtgaa attgactggc aacccaccgt gttctgggcg ctggcctaca gtgcgattct
     3001 ggcgacagcg ctggcgtgga gcttatggtt gtttgtattg aaaaacttgc ctgccagtat
     3061 tgccagctta agcacactgg ccgttcccgt ttgcggcgta ctcttttcct ggtggctgct
     3121 cggtgagaat ccgggggcag ttgaaggtag cggtattgtg ctgattgtac tggcactggc
     3181 gctggtgagc cgtaagaaaa aagaagccgt cagtgtaaaa aggatctgaa ttttttcttc
     3241 atgtggggag atctcttatt tagcaaaata ccgatagtgc cccaccatcc gccagctaaa
     3301 cagtacatct tcttcctgcg cgcctgcgcc aatgttatgt atcaccagcg gcgtaccgtc
     3361 gcgggcgaag ccatctgaca ccaccccaat atgtgccagc ccgttatcta atcgccagga
     3421 gacaatatcg cccgcttgat agtcactggg gttcttgctg gtggggcgtg ttttatcgtg
     3481 gcgggtaaac caggtttcca gattaggcac ccgacggtga tcgatgttgc tgtccgggcg
     3541 ctttaactgc catttttgcg ggtacgcagc aaaattcttc gccatatctt cgtgcaccag
     3601 tttctgcaaa tcgaccttct ggctgcgcaa tgcacggatc accacatcgg aacatacgcc
     3661 gcgttcttgc gggacatcac cgccaggata ggtaagctgc acatacgccg gatcgtaaaa
     3721 tagcgtgctg ccgatttgct gtctggcgcc gtctgcgatg gcaagattgg tgttggcctg
     3781 gatttgtacc acggttggtg gaacggcggg agattttaag gagtaactgg taaataccgt
     3841 cagaagactg aataacgcca gtgaagcttt catctgtttc gtccgtgaaa ataagagtca
     3901 cagattcgac cttccgggct aacgattgtt ctgtggagaa aatgtccaga agcggataaa
     3961 aaaacacccg aacaacgtgg aagtttcaga ttactggcac agcgtgccgg atgcggcgtg
     4021 aacgccttat ccggtctaca aaagtacgta aatttactat attgcagaga ttgcgtaggc
     4081 ctgataagcg tagcgcatca ggcaattttg cgttcaaact cccggacaag ccgggagttt
     4141 ggagtagatt attcccactc ttgcaggaaa cgctgaccat actggtcggc taccagcagt
     4201 gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt tgtggatggt ttcaccttct
     4261 gcacatgccg cttctgccac aattcgcatt tttgccggga catcttcttt aatatccagt
     4321 tgagcgagag ttattggcaa acctaccgca tggctaagcg cagctacggt ttcgatttcc
     4381 tccaccggcg cgttttccag aaccagctgc gtcagcgtac cgaatgccac tttttcacca
     4441 tgataatagt gatgcgcgtc cgggatagcg gtcaggccgt tatgtaccgc gtgtgccgcc
     4501 gccagcccgc cgctttcaaa accaacgcca ctcaaatagg tgttcgcttc aatcacgcgc
     4561 tccagcgccg gagtcactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc
     4621 agcagggtgt tgtagcacag ttcagccagt gccagcgctg cctgagtgca cttgccaccc
     4681 gccatggtgg tcgcgccgct acgggaacag gcacgcgctt caaaccaggt tgccagcgca
     4741 tcgccaatac ccgccgctaa cagacgcgcg ggcgcgccag cgacgatttt ggtgtcgaca
     4801 atgaccatat tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg
     4861 tagataacag acaatgcgct gcacggtgcg tcggtagaag cgatagtcgg tgcgatcgct
     4921 accggaacac ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccacca
     4981 ataccgagaa ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca
     5041 ttttgcgaac attcaccgcc aaacggcgca atttctacta ccagtccagc atctttaaag
     5101 cttttctcga cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag
     5161 cgttccgcca gtggcttcag gtactcgccc agacgattaa ttacatctgc gccctggatg
     5221 tatttacctg gtgattgaat aatgcggtcc ataattactc ctttagagat gagtagtgcc
     5281 aaatgcggca ttccagtcgt gctcgaactt ctctatagct gactctaccg caggggtgtt
     5341 aagcatttgt tgcgctacat ctaagggcag tgtgatggat tcacatcctg ccagcaaaca
     5401 atccagcgcc tgacgtggtg ttttaaagct ggcagccagc accatgcttt ctggcgcatg
     5461 catttccagt aacgcttgca actcctgtac agtacgaatg ccgtcaccgc cctgggcatc
     5521 tacgcggtta acgtatggag caacgtattt ggctccagcc agcgccgcca gtaatccttg
     5581 cgcggcgctg tacactgcgg ttcccagcgt agtaatgcct tctttcttca gcattttaat
     5641 tgctgccaga ccttcagagg ttaccggaat tttcaccaca atgcccggaa tagcgtcgcg
     5701 caggcgtttc gcttctttca ccataccctg cgcgtcgcgg ctcatggtct gagcaaacag
     5761 aatgccctca tcaccgatcg ctttttgcag gcgcggcagc acttcccaga tggactcctt
     5821 gctggcagcg ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag
     5881 acgttcgact tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac
     5941 agttccagtt catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc
     6001 gcattacgga attcttcgtg catgatgcga cgagcgaggc gcgagaaaat gcgcatatgt
     6061 tgatcgcccg cggcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg
     6121 ccccagcgca ctggcgcttg cagacgcgct acgctgatgg tggattgctc aatgtgttcc
     6181 gatttactgt ggggaatggc gaaactaaag cccagtccgg tagagaaaac ggcctcgcgc
     6241 gcccacaagt cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta
     6301 tcggtcatgc ctttgagcac ttcttctttg ctgcgccagt cgctttccag tgtgatgcac
     6361 tcggcggtga ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc
     6421 acttccagcg aagtacggca ggccattgcc tggttgagca actggcggca ctcacggcta
     6481 tcaagttgcg ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca
     6541 tccagcccta aaccgaccag caacggcagc acggaacctt tcgctcccag ttcaccgcac
     6601 agaccaatcc atttgccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag
     6661 aaggccggat tcaggctgtt gtagtgacga gtcaccttag cgttatcgcg atccactgcc
     6721 agtagatatt gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat
     6781 tgatcgatga tgaacatcac cgacggcact tccagcatga tgccgagctg gatcttctca
     6841 tcaaacggaa tgtgttcgtt acgcagttgc tgtttggctt ccgccagttt ttctttcacc
     6901 cataagatct cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagca
     6961 gaggcgcgca ggatcgatcg tagttgtgtg gtaaacaacg acgcgtattc ttcataaata
     7021 cgcaccgcgc gataaccgag gaacgggtta gcttctgcgg gaatgttcag ataatcaacg
     7081 ggtttgtcac caccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc
     7141 agcgcctgac aaaagatgtt gtacagctcg ctttcacccg gtgcactggt gcgatccata
     7201 tagagcattt cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc
     7261 gcttccacgg agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgc
     7321 gcttgctggg tcagccagac acgctgttgc tcacgcagag cgtcctgtac gcgggcttct
     7381 tgctgataat aacgggccac ggattcccct ggctcaacca caatcgcccc tgcgttgccg
     7441 tcgatataaa tcgtctgatg ctgccacgga gtaagggcat caatatccac acctaccagc
     7501 gtcggaatgt tgaacgaacg ggcaaggatc accgtatgtg atgtggtgcc gccacttttc
     7561 agtaacagac ctttgagatg atttttatcc agttcgagga actggctggg ggtcagttca
     7621 tcagccatac aaatggcggg ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg
     7681 tagatttgct ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg
     7741 taactgctgc tggaacgggc gaactcttcg caaaagtgat tcgcactggc aacaatcgct
     7801 tcggcgcagc ttaatccggc gctgacacct gccagtaaat gttcgcgcag ggaagtatcg
     7861 ccagccaggg atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga
     7921 cgaaactcta tgtttttcag taccagcgtc agaccgtttt ccagtgcgga ttgttcggcg
     7981 tcaacgcctt tggctgcggg aagattaccc agcgcattga gatctaaaga agagatcggc
     8041 gtcagaatgc cgcctgcgct accgctgcac acggtgcgag cgcggataat ttgcggattc
     8101 agattggtca gtgaaaccgg cagtggttcc agttcgtcag attttacttc cgccagcggt
     8161 gcgtcgcagt gggggaattc atcgcgcagc cattggctta aacgctggtg cgcttcctgt
     8221 tcgtcggccc cggaaatcag taactggcag ttatcgccca ccagcgtatc ggtgccaatc
     8281 agcgcaaggg cgcttttggc gttgcccttg cggtcagtgc gcaggttatg ccactcaatt
     8341 tgtgatgaaa aagtattaca cagcgtttca acgtggcttg ccggacgcgc atggacgccg
     8401 ttaggtagct cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt
     8461 tctgtttaca ggataagagc gcacggcaac ggcctgccat gtgacaaatc tgccaaaagc
     8521 tggacaaatg taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc
     8581 gtacttccgg attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa
     8641 gtccagtaaa tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttctt
     8701 ccatcaaaaa tatggacatg ggctttcgca agcccgggag cagcttatga atgagttggt
     8761 gcagatcctg aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt
     8821 cgtggtatcg ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt
     8881 gccggatgcc gtagccgatc cgaatctgaa aaaactgttt gatatcggcg ttgcaggttt
     8941 gacgctgatg gtgcctttcc tcgccgctta catcggttat tccattgcag aacgttctgc
     9001 gctggctcca tgcgctatcg gtgcctgggt tggtaacagc tttggcgcgg gcttctttgg
     9061 tgcgctgatc gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccagt
     9121 gcataaagtt ctgcgctcgg taatgccaat cttcatcatt ccaatcgtcg gcacactgat
     9181 taccgctggc gtcatgatgt gggggctggg cgagccggtg ggggcgttga ccaacagcct
     9241 gacccagtgg cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg
     9301 tctgatgctg gcgttcgata tgggcggccc ggttaacaaa gtggcctatg ccttcatgct
     9361 gatttgcgtt gctcagggtg tttataccgt ggtggctatt gccgctgttg ggatttgtgt
     9421 tccaccgctg gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga
     9481 gcgcgaaacc ggcaaggcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat
     9541 tcctttcgcc gctgccgatc cgctgcgtgt cattccttcc atcatggtcg gttcagtttg
     9601 tggtgcggta actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat
     9661 tgtgctgccg gtggttgaag gtaagttggg ttacatcgcg gcagtggctg tcggggcggt
     9721 tgtgacggca gtctgcgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcaac
     9781 tgaagagaag gaagacgacc tggatctgga ttttgaaatt aattaattga ggaacgagcc
     9841 atgacgaaaa ttatcgcagt aaccgcatgt ccttcaggtg ttgcccatac ttatatggcg
     9901 gcagaggcgc tggaaagcgc cgcgaaagcg aaaggctggg aagtaaaagt agaaacccag
     9961 gggtcgattg gtctggaaaa tgaactgact gcggaagacg tggcaagcgc cgatatggtg
    10021 attttgacca aagatatcgg catcaaattt gaagagcgtt ttgctggcaa gactatcgtg
    10081 cgtgtcaaca tcagcgacgc ggtaaagcgt gctgacgcca ttatgagcaa aattgaagct
    10141 cacctggcac aaaccgctta agttcttatc ccgcccgcaa gggcgggttc gctttcccac
    10201 aggagttcct catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc
    10261 gtgaaatctc gctggagcgg gcgttgcttt ataccgccag ccatcggcaa accgaagggg
    10321 agccggtgat aatgcgtcgg gcgaaagcaa cagcgtatat ccttgaacac gttgaaattt
    10381 cgattcgtga tgaagaactg attgccggta accgcaccgt aaaaccacgc gccgggatta
    10441 tgtcgccgga aatggaccct tactggctgc tgaaagaact ggatcaattc ccgacgcgtc
    10501 cgcaggaccg ctttgctatc agcgaagaag ataaacgtat ctaccgcgaa gagttgttcc
    10561 cgtactggga aaaacgttcg atgaaagatt tcatcaacgg gcagatgacg gatgaagtaa
    10621 aagccgcgac cagcacgcag attttcagca tcaaccagac ggataaaggc caggggcaca
    10681 ttattattga ttacccgcgc ctgctgaatc acgggctggg ggagctggtg gcacagatgc
    10741 agcaacattg tcagcaacag ccggagaatc acttttatca ggcagcgctg ttactgctgg
    10801 aagcctcgca gaaacatatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaagct
    10861 gcactgatgg cccgcgtcgc gaagaattgc tgactattgc ggagatctcc cgccataacg
    10921 ccgaacataa gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca
    10981 ttctgcaata cgaatccaac gccagttcgc tatcgttggg gcgcttcgac cagtatatgt
    11041 tgccgttcta tcagacatca ttaacccagg gcgaagatcc ggcattcctg aaagaactgc
    11101 tcgaatcttt atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc
    11161 gttatttcgc cggtttccct accggctata ccgcactgct cggcgggtta accgagaacg
    11221 gacgtagcgc ggtgaacgtg ctttcgttcc tttgccttga cgcttatcaa agcgtgcaat
    11281 taccgcaacc gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga
    11341 aaaccgcaga aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg
    11401 tagtgccagc gttcctcaac cgtggcgttt tgctggaaga tgcgcgcgac tattccgtag
    11461 tgggctgtgt ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt
    11521 ttaacctgct gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaac gctgcgctga
    11581 cttatgaagg tttactggaa cagatccgcg ccaagatcag ccactacatc accctgatgg
    11641 ttgagggcag caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctactct
    11701 cgtcttttat cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt
    11761 ataacttctc cggcgtacag gggatcggta tcgccaacct gagcgattct ctccatgcgt
    11821 tgaaagggat gatttttgat caacagcgtt taagttttga cgaattgctg tcggtattaa
    11881 aagccaactt cgccacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgcttcg
    11941 agaaatacgg taacgatatc gacgaggtgg ataacattag cgccgaactg ttacgccact
    12001 actgcaaaga agtggaaaaa taccagaacc cgcgcggcgg ctacttcacg ccgggatcgt
    12061 ataccgtttc tgctcatgtc ccgttgggag cggtggttgg cgcgacgcca gacggtcgct
    12121 ttgccggaga gcaactggca gacggtggct tgtcacccat gctgggtcag gacgcacaag
    12181 gaccaacggc ggtactgaaa tcagtcagta agctcgataa cacactgctt tctaacggta
    12241 cattgctgaa cgtgaaattc actccggcga ccctcgaagg tgaagcggga ttacgcaaac
    12301 tggccgactt cttacgggcg tttacccagc ttaagttgca gcatattcag tttaacgtgg
    12361 tgaacgccga cacgttacgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg
    12421 tgcgcgttgc cggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca
    12481 tcatccgccg gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg
    12541 cgcattttca acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc
    12601 ttttttaaag gctgtccgca tctttgccca tggtgtgcta atccggagtc gatctccggc
    12661 aaaatccaga cggtgcgcag agaggcgaaa tgtctgcact gcgcgaaatg tttgcgtgat
    12721 gcggatgaat gcccctccgg ggcatttgaa cggattggcc gcgatatcag ccttgacgcg
    12781 ctggaacggg aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg
    12841 ctttctggcg gcgaagtgtt aatgcaggcg gagtttgcga cccgtttttt acagcgactg
    12901 cggctatggg gtgtgtcatg cgccatcgaa actgccggag acgcgccagc cagcaagctg
    12961 ttaccgctgg cgaaattgtg cgatgaagtg ttgttcgatt taaaaattat ggatgcggct
    13021 caggcgcggg atgtggtgaa gatgaacctg caacgcgtgc tggagaatct gcgtttgctg
    13081 gtgagtgagg gtgtcaacgt gatcccgcgt ttaccgctga tccctggttt cacgctcagc
    13141 cgggagaata tgcagcaggc gctggacgtg ctgatcccgc tgaatatcaa gcagatccat
    13201 ctgttaccgt ttcatcagta cggcgagccg aaataccgcc tgctggggaa aacatggtcg
    13261 atgaaagagg ttgctgcgcc gtcgtcagcc gatgtggcaa cgatgcgtga aatggcagaa
    13321 cgggccggat ttcaggttac cgtgggaggt taaaatggca tatctggtgg cagtaaccgc
    13381 ctgcgtaagc ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca
    13441 gttagagaag tggggcgtca gcattgaaac tcagggcgcg ttgggaacgg agaatcgttt
    13501 agcggacgag gatatccgcc gggcggatgt tgcgctgtta attacggata tcgagcttgc
    13561 cggtgctgag cgatttgaac attgtcgcta cgtgcaatgc agcatctacg ctttcctgcg
    13621 tgagccgcag agggtgatga gcgcagtgcg caaagtgctt tctgcgccgc agcaaaccca
    13681 tcttattctg gagtagtcgg tttttctgtc agttggctgt ggtactgccg acgatattcc
    13741 gacggcgagc gttcggtatt tttacgaaac agacggcaga agtagttgct gtcgacaaag
    13801 ccgcaggcgt gcgccacttc ttttaccttc aggtcatagc ctttcaataa tgtcttagcg
    13861 tgctccagtc gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac
    13921 aggtgcgaga ggtaatttgg cgaaatatag aacgcctgtg cgacagattc gcgggtaagt
    13981 gcggaggcat agcgttcgtc gatataatca cgaatagctt cgaatagagc ctggctgcgt
    14041 gaggcggtct gtatttggct gccgagcaaa tcgcggcagt ggctaagcag gctggcgacg
    14101 ataagtctgg ccgtttgctg ctcctgcgac tgcatttgca tttcattgag cgtttgtagc
    14161 agaaaagaac caatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat
    14221 tgtttgccat cccattgcac gacgctgaag ccgagttgct gtttgccaaa tagcacgcta
    14281 aaggtcgtgg cgggggcttg ccattgtgga aaattccagc caccagcagg gacgtacagc
    14341 acatcgccgg gaactaacgc tgctccagcg ccggtatcaa taaactcacc ttccagcaca
    14401 atttccagac gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttgctgctg
    14461 gcaaagtaaa tctggcgcag ggacagcggg ccgttgatca ggcgggagag cagatagctg
    14521 acgtcgtgat acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat
    14581 gaaaaaaggc cacctgaaaa gtgtggcctg atgggagtga gatgtaaaca ttgcggcagt
    14641 ttcagtcgac ttgtattgcc ggatgcggcg taaacgcctt atccggccta ccgccgcgat
    14701 ctgtaggccg gataagacgc gtcaagcgtc gcatccggca aataacatct ggctgataat
    14761 tactgattac ccacctgatc gccatacggc agagtgtcgt aatcgatcag tgcgtttttc
    14821 ttatacgggt taccaatcca gcgggtagtt tctttgaact gcggattcac cactgaacgg
    14881 gttggatcgt aaccgtcgta agataagccc gccaaatctg accaggtgtg gatcagttcc
    14941 gccaggctgt atttacgatc aacatcctgt gagaaatcac gcggatgagt cgcttgccat
    15001 ttttctgacg tccacagcag gaacggaatg gtgtacatat gacgcgtcgg attgtcttca
    15061 ttacgcccct gagttttgtg cggcggcgtg tcgtaaacct cttcaccgtg gtcagagaaa
    15121 taaaccagga aaccgttcgg atcggtcgct ttaaagtctt taatcaggct ggcaaccacg
    15181 tgatcgttgt acaggttagc attgtcataa tcattatatg actccagctc ttctgcgttt
    15241 aatcccggtg gaacatgctc tgtattaccg tcaaatttgc cctgatcttc cggataacgg
    15301 tacttgtact tgatatgcgt acccagcaga tgaacgatga tcagtttctt cggcgcaggg
    15361 tccttcagca cttcctggaa cggcttcagc acgttggtgt cgtattcacg cgcactctgc
    15421 gtacgttgct ggttcatgta gtactgcttg tcggtctggc gcgaaaatac cgtcagcatc
    15481 gtattgcggg cggtcattgt ctgctggttg gtgatccaga aggttttata acccgcctgt
    15541 ttcatcatgt tcatcagcga cggctgcgtc agatacagat ccgggttctt ttcattggca
    15601 aaggtcagcg cctgttgcag gatttcaatg gtgtacggac gagacgtaac cacgttatta
    15661 aacacggtca gattcggatc ggttttatgc agcgcatcca gctccggcgt ggtttcacgc
    15721 ggataaccgt acaggctcat gcgtccacgc tgggtcgatt cgccaattac cagcactaag
    15781 gtgcgcggtt cgttacccga ttcatctttg aaattagcca gcggcggcaa tgcattattt
    15841 tcattcagta gctttgttag cgagtttagt tgctgacgat actgataata gccggtcagg
    15901 aattgccacg gtgcggcagg ctccatacgc gatgccaggt tatccaacgt tttctcaaac
    15961 ggcttgtttt tgataaacgt attcatggcg atcggatgca gaatcaagcc ataaagtagg
    16021 gcaaaagaga caacataacg ccacggcttt ggaatataga ccgggcgcag gcgtgtccac
    16081 agcagaactg ccaccgccgt ataggccagc gcgataagca caattttcag gctgaaatac
    16141 tggcttaaat actcgctggc ttcgttggtg ttggtttcga acatcacaaa cagaacgctc
    16201 tgcgagaact cctgaccgta gatgacgtaa tagcacagcg ccgccagaga ggccgcccat
    16261 agcaccacgc cgatcactgc ggcaataatt ttaatccgct tcggaaagag gaataccggg
    16321 atcaaccaca gcgaactgaa taacagcgag tcgcgaatgc cgttagtgcc actataacca
    16381 ctgatgtaaa taatggcctg tagcagagta gagaaaaacc aaaagtagag cagtgcccaa
    16441 cccagggctt tccagctaaa aaggggttta gcctggactt ctgtggaatg catagtaaga
    16501 acctgtcttg aaaaaaatat cgccgaatgt aacgacaatt ccttaaggat atctgaaggg
    16561 atatccagat tttgaataaa atgcagacag aaatatattg aaaacgaggg tgttagaaca
    16621 gcagtatttc agaaaaccct cgcgcaaaag cacgagggtt tgcaggagag gaagattagc
    16681 cggtattacg catacctgcc gcaatcccgg caatagtgac cattaacgcc tgctcgacgc
    16741 gaggatccgg ttcatggcct tctttttctg cctggcggga gcggtgcagc aactcggcct
    16801 gcaatacgtt cagcgggtcg gtgtaaatat tccgtagctg aatagactct gcaatccacg
    16861 gcagatcggc catcaggtga gaatcgttgg caatcgccag caccactttg atgtcttctt
    16921 cttgcagatt gcgtaactct ttacctaacg gccacagtgc tttgtcgacc aggcgttggt
    16981 catagtattc tgccagccac aggtctgctt tggcgaagac catctccagc atgccgagac
    17041 gcgtcgagaa gaatggccaa tcgcggcaca tggcttccag ctcgctctgt ttgccatctt
    17101 cgaccacttt ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc aggcggtttt
    17161 gcgtccaggc aaaaatccac ggaatggcgc gcagcgactc gacgccgccg gtcgggcgac
    17221 gtttcgccgg acgtgaaccc aacggcagtt tacccaattc ttgttccggc gtagcggagc
    17281 ggaagtaagg cacaaaatct ttgttttcac gtacgtagcc acggtagaga tcgcaggaga
    17341 tgactgacag ttcatccata atgcgacgcc agctctcttt cggctcaggc ggtggcagca
    17401 ggttggcttc cagaatcgcc ccggtgtaca gcgacaggct gctgacggtg atttctggca
    17461 gaccatattt aaagcggatc atctcgccct gttcggtcac gcgcaggcca cctttcaggc
    17521 ttcctggcgg ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga ccaatggaac
    17581 cgccgcgacc gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt ttgattaatg
    17641 catcctgtgc ctgatattgc gcccaggaag ctgccatcac gcccgcatct tttgctgagt
    17701 cggaatagcc aatcatcacc atctgtttgc cctgaatcag gccgcgatac cagtcgatat
    17761 tcagcagctg ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt tcaaacagcg
    17821 gagcaaccgg catcgcaaac ccgatacccg cttctttcag cagcaggtgg acagccagca
    17881 cgtcggacgg cgttttcgcc atcgagatca cgtaggcggc aatggagcct tgcggcgctt
    17941 cggcaatcac ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt tgccagttgc
    18001 gcggtagaag cggacgtttg gagttcagtt cgcggatcag gaacgcctgt ttgtcggctt
    18061 ctgaccagct ctcgtagtcg ccgataccga ggtagcgggt cagctcgccc agcgcttcgg
    18121 tatggcgggt gctctcctga cggatatcaa tacgcaccag cggtacgccg aaacatttca
    18181 cgcggcgcag ggtgtcgagc agatcgccat tggcgataat acccatgcca cacgcctgaa
    18241 gtgactggta gcaagcgtag agcggttccc acagttcttc gttttgtgtc agcagacctt
    18301 ctggttttgg cagttcttcg cctttcaggc gcgcttccag ccatgcctgt gtcgccatca
    18361 ggcgagaacg caggtttttc atcagatagc gatacggttc tgcggcaccc tcttcgccaa
    18421 ccagtgccag cagttcaggg gtggcttcaa ccatcgacag ttcagaaacc agaacctgaa
    18481 tatctttcag gaacaaatcg gtggctttcc agcggctgag tagcaggacg tggcgggtga
    18541 tatcggcagt gacgttcgga ttgccgtcgc ggtcaccgcc catccacgag gtaaaacgta
    18601 ccggaacaaa ttcgacgggc agtttgtagc cgaggttctc ttccagttgt tcgttcagtt
    18661 cgcgcaggta attcggtacg ccttgccaca ggctgttttc tactacggca aagccccatt
    18721 tggcttcatc taccgggctt gggcgcagtt tacggatttc atcggtatgc catgactggg
    18781 cgatcaactg gcgcaggcgg cgcatcagct ggttgtgttc gtagtcggcg atatctttgt
    18841 tatcgagctg ttttaaacag gcgttcactt ccaccatttt gtggatcagt gtacgacggg
    18901 taatttcggt tgggtgagcc gtgaggacca gttccagcga cagcgattcc actgcttttt
    18961 tgatggtgtc ttcgctcagt tccggctggt ttttcagttt acgcagggtg cgggcgatca
    19021 cttccgggtt gctggcagct tcgcctttcg gcgaaatgct gtggtattgc tcagcggtgt
    19081 tggccaggtt cagaaactga ctaaacgcac gcgcaacggg cagcagctcg tcgttcgaca
    19141 aattttgtaa ggtggtgagc aactcctggc ggttagcatc attgccagcg cgtgaagatt
    19201 tggacaactt acggatagtt tctacgcgtt caagaatgtg ttctcccaac gcatccttga
    19261 tggtttctcc cagcactttg ccgagcatac tgacattact acgcaatgcg gaatattgtt
    19321 cgttcatatt accccagaca ccccatctta tcgtttgata gccctgtatc cttcacgtcg
    19381 cattggcgcg aatatgctcg ggctttgctt ttcgtcgtct tttataaagc cacgtaaaag
    19441 cggtgacgtc aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag gaatttacgc
    19501 cattaaattc acgacgcttt aaataagcgt aacttatggg aatgttaaaa aatcgcctcg
    19561 agtaacacca aaggtgtagg tcggataaga tgcgcaagca tcgcatccga cattgtttgg
    19621 cctacgtttt aatgccagca aaaatggtga atcacctggg tgatcagttc gcgggtgggc
    19681 ttgataaacc tcgtctccag atattcatca ggctgatgag cctgattaat tgagccaggc
    19741 cccagaacca gcgtcgggca taacgtttga ataaacggcg cttcggtaca gtaattcacc
    19801 acttcggtct ttgctccgag caatttctca accacttcaa ccagtttatg attcggtggg
    19861 cattcatagc cagggatcgg cgggtgcagc tcgtcgaccg tcagacgacc cggccagcgt
    19921 tcgctcactg gagccaatgc atcgttgagc aaaccattaa gttcattgag tgtcatgcct
    19981 ggcagcggac gaatatccat atgcaactca caacaagcgc aaatacggtt agaggcgtcg
    20041 ccgccgtgaa tatgcccgag gttgagtgtg gggtatggca cggtaaacgc ttcatagtga
    20101 tagcgttctt tcaggttatc gcgcaattgc aaaatatggc cgatggcgtc gtgcatcagt
    20161 tcgatagcgt taaccccgcg cgacggatcg ctggagtggc ccgactgacc cataattcga
    20221 atggcatttg agatgtggcc tttatgtgcg cgtaccggtt gcagcgacgt tggctcgcca
    20281 atgatggcgc aatccgggcg cagggcggta gtttcggcaa aataacgcgc tccggccata
    20341 ctggtttctt catcggcagt cgccagaatg tagagcggtt ttgcaagctt cgtgacgtcg
    20401 acatcgcgta gcgcatcaag gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag
    20461 ccgtaaagct tgccgtcatg ctccgtcaac gtaaatggat cgcgcgtcca gcgtccgtca
    20521 tcaaatggca ccgtatcggt atgccccgcc agcaacaaac cgccagcccc ctgtccgcaa
    20581 ctggccagca tattaaattt gttgcgagtt cctggcacag actgcacttc cacattgaag
    20641 cctaaatcct taaaccagtc cgccagcaga gtgattaaat ctgcattgct ttgatcgagt
    20701 gcctcttccg tggcgcttat tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat
    20761 ggcggtaatt tgtttttcat tgttgacaca cctctggtca tgatagtatc aatattcatg
    20821 cagtatttat gaataaaaat acactaacgt tgagcgtaat aaaacccacc agccgtaagg
    20881 tgaatgtttt acgtttaacc tggcaaccag acataagaag gtgaatagcc ccgatgttga
    20941 atacgctgat tgtgggtgcc agtggctacg ctggcgcaga gctagtgacc tatgtaaatc
    21001 gccatccgca tatgaacata accgctttga ctgtttcagc gcaaagcaat gatgcgggaa
    21061 agttaatctc cgatttgcat ccgcagctaa aaggcatcgt tgatctgccg ttgcagccga
    21121 tgtcggatat cggcgagttt agcccagggg tggacgtggt gtttctcgcc accgcccatg
    21181 aagtcagcca cgatttagcg ccgcaatttc ttgaagcagg ctgtgtggtg ttcgaccttt
    21241 ccggcgcgtt tcgtgttaac gatgtcgcct tctatgaaaa atattacggc tttacccatc
    21301 aatacccgga actgctggaa caggcagcct acggtctggc ggagtggtgc ggtaataaat
    21361 taaaagaagc gaacttgatt gccgtgccag gctgttatcc gacggcagca cagctggcgc
    21421 tgaaaccgtt gattgatgcc gatcttcttg acctcaatca gtggccggtg atcaacgcca
    21481 ccagcggcgt gagcggtgca ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta
    21541 gcctgcaacc gtatggcgtc tttacccatc gccatcaacc agagatcgcc acacacctcg
    21601 gtgctgacgt tatcttcacc ccgcatctgg gcaatttccc gcgcggcatt ctcgaaacca
    21661 ttacctgccg cctgaaaccg ggtgtgagcc aggtgcaagt ggcgcaagcg ttacagcagg
    21721 cgtatgccca taaaccactg gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg
    21781 tcgttggact gccattctgc gatatcggtt ttgccgttca gggcgagcat ctgattattg
    21841 tggcgaccga agacaactta ctgaaaggcg cggcggcaca agcggtgcag tgcgccaata
    21901 ttcgtttcgg ctatgcggaa acgcagtctc ttatttaagg gtgcaatgat gaatccatta
    21961 attatcaaac tgggtggcgt actgctggat agtgaagagg cgctggaacg tctgtttagc
    22021 gcactggtga attatcgcga gtcacatcag cgtccgctgg tgattgtgca cggcggcggt
    22081 tgtgtggtgg atgagctgat gaaagggctg aatctgccgg tgaaaaagaa aaatggcctg
    22141 cgggtgacgc ctgcggatca gatagacatt atcaccggag cactggcggg aacggcaaat
    22201 aaaaccctgc tggcatgggc gaagaaacat cagattgcgg ccgtaggttt gtttctcggt
    22261 gacggcgaca gcatcaaagt gacccagctt gatgaagagt taggccatgt tggactggcg
    22321 cagccaggtt cgcctaagct tatcaacagt ttgctggaga acggttatct gccggtggtc
    22381 agctccattg gcgtaacaga cgaagggcaa ctgatgaacg tcaatgccga ccaggcggcg
    22441 acggcgctgg cggcaacgct gggcgcggat ctgattttgc tctctgacgt cagcggcatt
    22501 ctcgacggca aagggcaacg tattgccgaa atgaccgccg cgaaagcaga acaactgatt
    22561 gagcagggca ttattactga cggcatgatt gtgaaagtga acgcggcgct ggatgctgcg
    22621 cgcacgttgg gccgtccggt agatatcgcc tcctggcgtc atgcggaaca gcttccggca
    22681 ctgtttaacg gtatgccgat ggggacgcgg attttagctt aagttttgtt ggccggaggc
    22741 gcagctttcc ggcattgaat ttcaaaataa ggaaacagag ttatggcact ttggggcggg
    22801 cgttttaccc aggcagcaga tcaacggttc aaacaattca acgactcact gcgctttgat
    22861 taccgtctgg cggagcagga tattgttggc tctgtggcct ggtccaaagc cctggtaacg
    22921 gtcggcgtgt taaccgcaga agagcaggcg caactggaag aggcgctgaa cgtgctgctg
    22981 gaagatgttc gcgccaggcc acaacaaatc cttgaaagcg acgccgaaga tatccatagc
    23041 tgggtggaag gcaaactgat cgacaaagtg ggtcagttag gcaaaaaatt gcataccggg
    23101 cgtagccgta atgatcaggt agcaactgac ctgaaactgt ggtgcaaaga taccgttagc
    23161 gagttgctga cggctaaccg gcagttacaa tccgctctgg tcgagaccgc gcagaacaat
    23221 caggacgcgg tgatgccggg ttacacccac ctgcaacgcg cccagccggt gacgttcgcg
    23281 cactggtgcc tggcctacgt tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg
    23341 cttaagcgtc tggatgtcag cccgctaggc tgtggcgcac tggcaggaac ggcctatgaa
    23401 atcgaccgtg aacagttagc aggttggctg ggctttgctt cggcgacccg taacagtctc
    23461 gacagcgttt ctgaccgtga ccatgtgttg gaactgcttt ctgctgccgc tatcggcatg
    23521 gtgcatctgt cgcgttttgc tgaagatctg attttcttta acaccggcga agcggggttt
    23581 gtggagcttt ctgaccgtgt gacttccggt tcatcattaa tgccgcagaa gaaaaacccg
    23641 gatgcgctgg agctgattcg cggtaaatgc ggtcgggtgc agggggcgtt aaccggcatg
    23701 atgatgacgc tgaaaggttt gccgctggct tacaacaaag atatgcagga agacaaagaa
    23761 ggtctgttcg acgcgctcga tacctggctg gactgcctgc atatggcggc gctggtgctg
    23821 gacggcattc aggtgaaacg tccgcgttgc caggaagcgg cgcagcaggg ttactccaac
    23881 gctactgaac tggcggatta tctggtggcg aaaggcgtac cgttccgcga ggcgcaccat
    23941 attgtgggtg aagcggtggt ggaagccatt cgtcagggca aaccgctgga agatctgcca
    24001 ctcagtgagt tgcagaaatt cagcctggtg attggcgaag atgtctatcc gattctgtcg
    24061 ctgcaatcgt gcctcgacaa gcgtgcggca aaaggcggtg tctcaccgca acaggtggcg
    24121 caggcgattg cttttgcgca ggcgcggttg gggtaagaac atttatatgt ataaatttga
    24181 gcctggctta tcgctgggct tttttatggc aaaaaaaagc ggatcctgga gatccgcaaa
    24241 agttcacgtt ggctttagtt attcgagctg agaaactctc gaaacgggct gtgacttcaa
    24301 gggttaaaag aggtgccgct ccgtttctgt gagcaattat cagtcagaat gcttgatagg
    24361 gataatcgtt cattgctatt ctacctatcg ccatgaacta tcgtggcgat ggaggatgga
    24421 taatgaatat tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc
    24481 gtgcggcaga ttcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg
    24541 aagatgagct gggcgtgatg ttgctggagc ggaccagccg taaagtgtta ttcacccagg
    24601 cgggaatgct gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag
    24661 agatggcaag ccagcagggc gagacgatgt ccggaccgct gcatattggt ttgattccca
    24721 cggttggacc gtacctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc
    24781 tggaaatgta tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagcggca
    24841 aactcgattg cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt
    24901 tgtttgatga gccaatgttg ctggctatct atgaagacca cccgtgggcg aaccgcgaat
    24961 gcgtaccgat ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt
    25021 tgcgcgatca ggcaatgggt ttctgttttg aagccggggc ggatgaagat acacacttcc
    25081 gcgcgaccag cctggaaact ctgcgcaaca tggtggcggc aggtagcggg atcactttac
    25141 tgcctgcgct ggctgtgccg ccggaacgca aacgcgatgg ggttgtttat ctgccgtgca
    25201 ttaagccgga accacgccgc actattggcc tggtttatcg tcctggctca ccgctgcgca
    25261 gccgctatga gcagctggca gaggccatcc gcgcaagaat ggatggccat ttcgataaag
    25321 tgttaaaaca ggcggtttaa gccatttagc gcagctaccc gataggcttc cgccatcgtc
    25381 gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc
    25441 ataatcgcct gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaataccc
    25501 agaatctctt ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg
    25561 atttgtgcac gtgccagatg tttaaactgg gcgcggccta cttcatatgg cactttcatc
    25621 gcggtcagct gctgttcggt tttgcctaca gagctgattt ccgggatggt gtaaatgccg
    25681 gtagggatat cttcaatcag atgtgcggtg gcttcacctt tcaccagcgc ctgcgcggca
    25741 atgcgcccct ggtcataggc tgccgacgcc aggctcggat aaccaatcac gtcgcccacc
    25801 gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg
    25861 tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg tattaccggt gcgaccgttg
    25921 gcatagagca gacagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg
    25981 tcatcacagc cttcgatctt ctcgtactct tcgttgtgac gaatcactac gccactgttc
    26041 cagaagtgat aggagagaga atctgacatc tcttgatcga gaaatgccag caggcgatcg
    26101 cgggtgttga tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag
    26161 ccgatcactc cagcaccgta aataagtaca tggcgcggtt cgtggtgcat gctaagaatt
    26221 gagtcgctgt cataaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga
    26281 gaaccgcagg caataacaaa tttttcagca gttagtgttt cgacgctgcc gtccgggcaa
    26341 tccagcgcca acgtatgctc atcaacaaag cgagcgttcc cctgcaatat ttcacagtga
    26401 ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg
    26461 ttaaggatat cggcaaaaga agagcggagc agtcgggaat ggtcgctgta tagtgggttt
    26521 tgattgaatt ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc
    26581 cagtgggtgc aaccgccgcc aacattttga taacgctcga taactgcgac gcgcgcacct
    26641 tgcttaacca ggcccatcgc agcgccttcg ccgccggggc cggaacctat tactatggca
    26701 tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag
    26761 aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg tcatttatct ggctatatcg
    26821 tgagcggcct ttgctttgtc ttcctccttc ttattttatt tattcactat taatcgcttt
    26881 ttcttatggc tgatagtttt atcttgccga agcgtgcttt tttgttattg tctttatgaa
    26941 atcttcttat aaaacaaatg tataaatatt ttatattgat gggtt
//
LOCUS       DACADA010000055        26956 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.056, whole genome shotgun sequence.
ACCESSION   DACADA010000055 DACADA010000000
VERSION     DACADA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.056"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            59..502
                     /gene="cmtB"
                     /locus_tag="ISB17_002235"
     CDS             59..502
                     /gene="cmtB"
                     /locus_tag="ISB17_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAP1980167.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     SIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            530..1918
                     /locus_tag="ISB17_002236"
     CDS             530..1918
                     /locus_tag="ISB17_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP1980168.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLFCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1933..3210
                     /locus_tag="ISB17_002237"
     CDS             1933..3210
                     /locus_tag="ISB17_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1980169.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3207..4172
                     /gene="yggF"
                     /locus_tag="ISB17_002238"
     CDS             3207..4172
                     /gene="yggF"
                     /locus_tag="ISB17_002238"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP1980170.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4194..4703
                     /gene="fumE"
                     /locus_tag="ISB17_002239"
     CDS             4194..4703
                     /gene="fumE"
                     /locus_tag="ISB17_002239"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAP1980171.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4700..5413
                     /locus_tag="ISB17_002240"
     CDS             4700..5413
                     /locus_tag="ISB17_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAP1980172.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5385..6062)
                     /locus_tag="ISB17_002241"
     CDS             complement(5385..6062)
                     /locus_tag="ISB17_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1980173.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(6056..6733)
                     /locus_tag="ISB17_002242"
     CDS             complement(6056..6733)
                     /locus_tag="ISB17_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP1980174.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6721..7428)
                     /locus_tag="ISB17_002243"
     CDS             complement(6721..7428)
                     /locus_tag="ISB17_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP1980175.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7429..8007)
                     /locus_tag="ISB17_002244"
     CDS             complement(7429..8007)
                     /locus_tag="ISB17_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP1980176.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8030..8461)
                     /locus_tag="ISB17_002245"
     CDS             complement(8030..8461)
                     /locus_tag="ISB17_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1980177.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8833..9852
                     /gene="epd"
                     /locus_tag="ISB17_002246"
     CDS             8833..9852
                     /gene="epd"
                     /locus_tag="ISB17_002246"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAP1980178.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9902..11065
                     /gene="pgk"
                     /locus_tag="ISB17_002247"
     CDS             9902..11065
                     /gene="pgk"
                     /locus_tag="ISB17_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP1980179.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11279..12358
                     /gene="fbaA"
                     /locus_tag="ISB17_002248"
     CDS             11279..12358
                     /gene="fbaA"
                     /locus_tag="ISB17_002248"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP1980180.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12549..13409
                     /locus_tag="ISB17_002249"
     CDS             12549..13409
                     /locus_tag="ISB17_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAP1980181.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13548..14183
                     /gene="argO"
                     /locus_tag="ISB17_002250"
     CDS             13548..14183
                     /gene="argO"
                     /locus_tag="ISB17_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAP1980182.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14276..15016
                     /locus_tag="ISB17_002251"
     CDS             14276..15016
                     /locus_tag="ISB17_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAP1980183.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15184..15762
                     /locus_tag="ISB17_002252"
                     /pseudo
     CDS             15184..15762
                     /locus_tag="ISB17_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15769..16080
                     /locus_tag="ISB17_002253"
     CDS             15769..16080
                     /locus_tag="ISB17_002253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980184.1"
                     /translation="MRFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGH
                     FLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTN
                     H"
     gene            complement(16077..17132)
                     /locus_tag="ISB17_002254"
     CDS             complement(16077..17132)
                     /locus_tag="ISB17_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAP1980185.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(17335..18228)
                     /locus_tag="ISB17_002255"
     CDS             complement(17335..18228)
                     /locus_tag="ISB17_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAP1980186.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18369..18599
                     /locus_tag="ISB17_002256"
     CDS             18369..18599
                     /locus_tag="ISB17_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980187.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18655..19314
                     /gene="rpiA"
                     /locus_tag="ISB17_002257"
     CDS             18655..19314
                     /gene="rpiA"
                     /locus_tag="ISB17_002257"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP1980188.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19570..20802
                     /locus_tag="ISB17_002258"
     CDS             19570..20802
                     /locus_tag="ISB17_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP1980189.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21031..21090
                     /gene="ibsC"
                     /locus_tag="ISB17_002259"
     CDS             21031..21090
                     /gene="ibsC"
                     /locus_tag="ISB17_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAP1980190.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21191..21793)
                     /locus_tag="ISB17_002260"
     CDS             complement(21191..21793)
                     /locus_tag="ISB17_002260"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP1980191.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21814..21997)
                     /gene="ssrS"
                     /locus_tag="ISB17_002261"
     ncRNA           complement(21814..21997)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ISB17_002261"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22039..22368)
                     /gene="zapA"
                     /locus_tag="ISB17_002262"
     CDS             complement(22039..22368)
                     /gene="zapA"
                     /locus_tag="ISB17_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP1980192.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22536..23114
                     /gene="ygfB"
                     /locus_tag="ISB17_002263"
     CDS             22536..23114
                     /gene="ygfB"
                     /locus_tag="ISB17_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAP1980193.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23140..24465
                     /gene="pepP"
                     /locus_tag="ISB17_002264"
     CDS             23140..24465
                     /gene="pepP"
                     /locus_tag="ISB17_002264"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP1980194.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24462..25640
                     /gene="ubiH"
                     /locus_tag="ISB17_002265"
                     /gene_synonym="visB"
     CDS             24462..25640
                     /gene="ubiH"
                     /locus_tag="ISB17_002265"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAP1980195.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25663..26865
                     /gene="ubiI"
                     /locus_tag="ISB17_002266"
                     /gene_synonym="visC"
     CDS             25663..26865
                     /gene="ubiI"
                     /locus_tag="ISB17_002266"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAP1980196.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6523 a         6559 c         7278 g         6596 t
ORIGIN      
        1 cacgagtatt ttatttttac cgccggacgg taccctaaaa ataaaataag gataatttat
       61 gcggcttatt gattattttc cagaatcatc aatctcagtt atacattcag caaaagactg
      121 gcaggaagct atcgatttct cgatggcatc attgctggat aaaaactata tcagcgagaa
      181 ttacattcaa tctattaaag actccaccat cagcaatggt ccttattata ttctcgcacc
      241 aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt
      301 gacgttactt aaacaaggcg tttatttccc ggggaaggac gaaccgatta aattactcat
      361 cgggctttct gctgccgatg ccaattcgca cattggcgct attcaggcgt taagtgaatt
      421 actgtgcgaa gaagaaatac tcgaacaact cttaacagca tcatcagaaa aacaattagc
      481 ggacattatc agccgcggat aattactctc tcctctacag gataatatta tggaaaacaa
      541 gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg actgcaatgg tcatccccaa
      601 tatcggtgct tttattgcct ggggatttat aacggcatta tttattccca ccggctggct
      661 gccgaatgaa catttcgcca aaattgttgg tccgatgatt acctatttat tgcccgtgat
      721 gattggttct accggtggtc atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat
      781 tggtacgatt ggtgtgatcg tcggcgctga aatcccgatg ttccttggct cgatgattat
      841 ggggccgctc ggtggtctgg tgattaaata tatcgacaag tcgctggaaa aacgcatacc
      901 tgctggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga tgctcttttg
      961 tctgctgggt tttgaagtta tcggtccggc ggtgttaatt gctaatactt tcgtcaaaga
     1021 gtgtattgag gcgctggtac atgcgggtta tctgcctttg ctgtcagtca tcaatgaacc
     1081 ggcgaaagtg cttttcctta ataatgcgat tgatcagggc gtctattatc cgctgggaat
     1141 gcaacaggct tcggttaacg gtaaatccat cttcttcatg gtggcctcca acccaggtcc
     1201 tggtctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta aacgttctgc
     1261 gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt tcccgtatgt
     1321 gctgatgaag ccgctgacca ttattgccat gattgcgggc ggtatgtctg gcacctggat
     1381 gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta tctttgctta
     1441 cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta cggtaggtac
     1501 cctggtgtcc tttgctatca cttcgctgat actgaagatg gaaaaaacgg tggaaacgaa
     1561 gagcgaagat gagtttaccg agtcagccaa tgcggttaag gcgatgaaac aagaaggtgc
     1621 attctcgtta agccgtgtta agcgtattgc ctttgtttgc gatgcaggga tgggctccag
     1681 tgcgatgggc gcaaccacct tccgtaaacg cctggaaaaa gcggggctgg caatagaagt
     1741 aaaacattac gccatagaaa acgtgcctgc ggatgcggat atcgtcgtta ctcacgccag
     1801 tctggaaggg cgcgtgaaac gtgtgacgga taaaccactg atattgatta ataactatat
     1861 tggcgatcca aaactcgaca ctttatttaa tcaattaacc gctgaacata aacactgatt
     1921 acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga tgtccgtctg
     1981 cgcgaatttg aactgccaga aattactgat aatgaactgt tagtgagtgt aatttctgac
     2041 agcgtttgtt tatccacctg gaaagcggcg ttgcttggta gtgaacataa acgcgtcccc
     2101 gacgatttag aaaatcatcc ggttattacc gggcatgaat gtgctggggt tgttgtcgaa
     2161 gtgggtaaaa atctcactgg caaatataaa aaagggcagc gttttgtatt gcaaccggcg
     2221 atggggctac caagcggata ttcagccgga tacagctacg aatattttgg tggcaatgcc
     2281 acctatatga ttattcccga aatcgcgatt aatttaggct gtgtattacc gtatcacggc
     2341 tcttattttg ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat
     2401 gccaattatc acaccacgca atatgtttat gagcatcgta tgggcgtcaa acctggcggc
     2461 aatattgccc tgctggcgtg tgctggcccg atgggcattg gcgctatcga ttacgccatt
     2521 aacggcggca tacaaccggc gcgggtggtg gtagtcgata tcgacgacaa acggctggcg
     2581 caggtgcaga agttgctgcc ggtggagctg gcggccagta aaggcattga gctggtgtat
     2641 atgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac ggatgatgcc
     2701 ggatttgatg acgtatttgt ctatgcagcg gtgccttcag tcgtcgagat ggctgatgaa
     2761 ttactggctg aagatggctg cctgaacttc tttgccggtc cgacggataa aaacttcaaa
     2821 gtgccgttta atttctacaa cgtccattac aacagtacgc acattgtcgg cacgtctggc
     2881 ggttccacgg acgacatgaa agaggcgatt gcccttagcg ccacggggca gttacagccg
     2941 tcgtttatgg tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg
     3001 ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctgaccgcc
     3061 attgccgatt ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagct ggcgcggctg
     3121 gttgagaaaa cgcacggtat ctggaatgaa caggccgaga aatatctgct ggcacaattt
     3181 ggcgttgata ttggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta
     3241 cggaacaggc agcgttggct gcctggccgc aaaccggctg tggcgacaaa aataaaattg
     3301 atggccttgc ggtcaccgcg atgcgccagg cattaaacga cgttgctttt cgtgggcgag
     3361 tggttatcgg cgagggggaa attgaccatg cgccgatgct gtggattggc gaagaggtgg
     3421 gcaaaggcga tgggccagaa gtcgatatcg cggtcgatcc cattgaaggc acgcggatgg
     3481 tggcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcga gatagcctgt
     3541 tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga
     3601 tagatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac
     3661 cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc gcaattgagg
     3721 aagcaacaca gctcggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg
     3781 tgctgacttg ctggcaggat aatccttacg atgtgatgta caccatcggt ggcgcaccgg
     3841 aaggtgttat ttctgcctgt gccgttaaag cattaggcgg ggatatgcag gccgagctga
     3901 ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc
     3961 gcaagcgttg caaggcaatg ggcgttgacg ttaaccgtgt atactcgctc gatgaactgg
     4021 tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg
     4081 gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc
     4141 aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga
     4201 cgctgacaga agatgatgtg cttgagcaac tgaatgcaca ggacaattta ttttcattta
     4261 tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg
     4321 tcgataacga tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc
     4381 cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat
     4441 ggctgtacgc cgatatcacg catttttccc agttttggca ttacctgaac gaacaggatg
     4501 aaacgcccag atttgctgat gacataacct gggattttat cagcaacgtc aatagcataa
     4561 cccgcaatgc gacgctctat gacgcgttga aagcgatgaa gttcgccgat ttcgccgtct
     4621 ggtcagaggc gcgttttagc gggatggtca aaacggcgct gacgctggca gtaacgacca
     4681 ccttaaagga attaacgccg tgaaaatcga actgacggtg aatggactaa aggtacaggc
     4741 gcagtactct gatgatgaaa ttgaaaatgt acataaacca ttgctgcgta tgctggcggc
     4801 cttacagaca gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac
     4861 gggtaaatct accctaacca ccttctggga atatctcgcg caacaagacc cggaactgcc
     4921 cgctattcag acgctgccga tggatggttt tcaccattac aatagctggc tggatgtgca
     4981 tcaattgcga cccttcaaag gcgcaccaga gacattcaac gttgcgaaac tggcggaaaa
     5041 tctgtgccgg gttgtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga
     5101 tcctgttgaa gatgtgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt
     5161 gttgttggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat
     5221 caaagctccc gcatctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggagggtt
     5281 atcgctggca gacgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt
     5341 gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg
     5401 tctgatggat taacgttcct gattacacca tcttccagtc gcaccacacg agagaaatga
     5461 cgccgggtaa atgcggcgtc atgactgatt gctacgacgc tcgtgcctcg ctggcggcac
     5521 ttttccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg
     5581 tccagtagca atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc
     5641 tgcgcactat gcagatctaa cggatgcgcg ctggcagtgt ctgctaactg acagcattgc
     5701 agggcagcat tggtacgttg agtgatttca tccgccgggc atttttgcag tatcaaacca
     5761 aatgcgattt cgtcagcaac ggtgctgtga aaaagctgat tttccgcttc ctgaaacagt
     5821 acgccaatct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcttg ttgttgcagc
     5881 ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt
     5941 cccgcgccat tgtcgccggt cagcgccagc cattcacctt gtttgagttg cagagaaatg
     6001 tcgcaaagac aatccgtagc cgcgccaggc cagcgataag atatctgatt taacgttaac
     6061 atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtgg ttcaggaaag
     6121 gtgtgtgatg acgttcaaaa agaataatca gcgaaccacg ttcaagcgcc cagtgttgta
     6181 agcgctgcaa cagcatttcg cgggcctgcg gcgtcaggcg gctaaaggct tcatcaagaa
     6241 ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacacgctgt gtctctccac
     6301 cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcagg gccagggcgg
     6361 cgtcaatccg cgccataatc tctttttcag caagacacag attttctggc ccaaaggcaa
     6421 cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat tgctggacga
     6481 gctggatggt ggcggattgt tcattaagcg ggagacgccc aatgggcgta ccgagaagcg
     6541 tccccgtgcc ggttatctct cctggtagaa agtccggata ccagcccgcc atcagctgtg
     6601 ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc ggtttgacat
     6661 agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaatag cgaaactgtt
     6721 ctaacgtaac catatccacg ctccgaattc agccagcatt agcaatatca tggcgtaacg
     6781 tgctacgcat tgtagggtgc tatcggctgg tgcccataac gtagtgcgcc tattgttgat
     6841 tcgaaatgcc cgcatatcca gcgccgcacc acggacagca agatcgttta gcgcattgtg
     6901 tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc cctcatccag
     6961 cggcacgccg cgggcgcact gcgcttcatg aataatcgcc aattgccgtt ttaactgctc
     7021 aacgaccaat aacggcccgg caaacagata agcgacgccc ggcggcaggc gagaagcaaa
     7081 caaagcgcgg ataaaacgtt gcacggggac gtactgcatc cacaactgtg aggttgaaac
     7141 aatcgccaga atccgcaacc agagcgtaat agcatgcgcc caccgttctg gagagtgtgg
     7201 cgtgccgctt agccattctg tcagccagcc accgtgcacc agccacagtc cagcccccag
     7261 cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta gcgcaataag
     7321 gcagaaaaag gtcgccgcgc tatagactgg caatattgtc tgtgtaggca ggataagcgt
     7381 ggtgcaggcc gccagcgccc acagcgttaa ggatgtaaaa ggatgcattt agcgcacggc
     7441 agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tgacgcagta aaagccagac
     7501 tatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga
     7561 ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg ctccgcttcc
     7621 tgtcacgccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt
     7681 aataatcacg ccgctaacaa cgacttttgg cagggtgcga aaccaacccg ctcgcgccag
     7741 ccaaccggcg accaggccga tgaccatcgc gacgggcgca aatgcggcgg cgatcgggtc
     7801 ggtcagcaat ccccagagta aattggtcag taaaccggtc agcatcccga ttatcggacc
     7861 aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aaaataggta atttaaccat
     7921 gctggccagt tgcccgccga tcatattgat ggcgatagag ataacgatca acaccagtgc
     7981 ctgactggaa aagtggcgac gcgccataag aaaacctctc tgaaaatagt tatttaacgg
     8041 gggaaatatg caactcgtcg taaccagaaa gcgcgcaaag ctggcggcta aacgccagtt
     8101 cctcaaggca gccgatcacc agttcaagcg tggtccgcac ggtacagccg ggcatcatat
     8161 gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgt tcaccactct
     8221 gttccagcgt gccgttcaat gcgatgactt cgaacttacc gcttagcagg gcagtgtttt
     8281 cttgcccggc atagcgtaag gcgacgtcag tcagactgcc ggtacaacct gcaatccacg
     8341 cggcgtgaag ttgttgttgc tgcgcgaagg cgcgtaattg ggagagaact tcctgcccgg
     8401 ggagcaggcg cagcgcgtaa aagcgtgcgg aggaagaata tgggtagggc aatgtcatca
     8461 ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat cagtcatatt
     8521 ctgatataag tcagggtttg tgaccgcaca acgaaaagat attgctacgc ttattcgtga
     8581 ttctgcaaag tggaataaat tccacagggg cggggatgaa aacgtgacat aagtcagata
     8641 attgtcttct ttgactggac aaacattcct tttattccac gtttcgctta tcctagctga
     8701 agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt gcgacaggta
     8761 aggtttcccc gaacgatttg ctggattact ctgtctggca aatttgctca gggaaaacct
     8821 tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat
     8881 gtggttcgtg ctttgtatga atccggacgc cgtgcggaaa ttaccgtggt ggcaatcaac
     8941 gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag ccatggccgt
     9001 tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga cgccatccgt
     9061 gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc
     9121 ctcgactgca ccggcgtata tggctcccgc gagcatggtg aagcacacat tgccgccggg
     9181 gctaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac cgttgtttat
     9241 ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaatgc ttcctgtacc
     9301 acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat tgagtctggc
     9361 actgtgacca caattcactc cgccatgcat gatcaacagg ttattgatgc ataccatcct
     9421 gacctgcgtc gcactcgggc agccagtcag tcgatcattc cggtcgatac taaactggcc
     9481 gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaggcgat tgcggtacgt
     9541 gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa
     9601 gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt
     9661 gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca cagtgccatt
     9721 gtcgatggca ctcaaacccg ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc
     9781 gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat ggctactgtt
     9841 gctttcaggt aagacgcaag cagcgtctac aaaactttta gaatcaacga gaggattcac
     9901 catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc
     9961 ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc
    10021 tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct
    10081 gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg ttgttaacta
    10141 cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg acggcgttga
    10201 cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa
    10261 agacgacgaa actctgtcca aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc
    10321 attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat tcgctgacgt
    10381 tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag cactgaaaga
    10441 acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca aactgaccgt
    10501 tctggactcc ctgtctaaaa tcgctgacca gctgatcgtt ggtggtggta ttgctaacac
    10561 ctttattgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg acctggttga
    10621 cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc
    10681 aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg tgaaagctga
    10741 cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa
    10801 tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga acttccgcaa
    10861 aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca tcgctggcgg
    10921 cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct cctacatctc
    10981 cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat
    11041 gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc ccttgtttca
    11101 gcgcgctttt agagcacgca ctttttcata ctctaaataa ttcgagttgc aggaaggcga
    11161 caagagagtg aatcccagga gcttacataa gtaagtgatt ggggtgagcg aacgcagacg
    11221 cagcacatgc aacttgaagt atgacgagta taaggcccga cgatacagga caagagacat
    11281 gtctaagatt tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt
    11341 tttccaggta gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga
    11401 ctccatcaac gccgtgctgg aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt
    11461 ctccaacggt ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg
    11521 tgctgctatc ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta
    11581 tggtgttccg gttatcctgc acaccgacca ctgcgcgaag aaactgctgc cgtggatcga
    11641 cggtctgctg gacgcgggtg aaaaacactt cgcagctacc ggcaagccgc tgttctcttc
    11701 tcacatgatc gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata
    11761 cctggagcgc atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg
    11821 tgaagaagac ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga
    11881 agacgttgat tatgcgtata ccgagctgag caaaatcagc ccgcgtttca ccatcgcagc
    11941 gtccttcggt aacgtacacg gtgtttacaa gccgggtaac gttgttctga ctccgaccat
    12001 cctgcgcgac tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgaa
    12061 cttcgtattc cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta
    12121 cggcgtagtg aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct
    12181 gaactactac aaagcgaacg aagcttacct gcagggtcag ctgggtaacc cgaaaggcga
    12241 agatcagccg aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc
    12301 gatgatcgct cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaagt
    12361 cattcttttc tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg
    12421 tgatgcagat gagaaatctg tgatctattt ggcaaaatta tgctttattg tttacccttg
    12481 tcagactgcc cgccataagg cggcggagtg tatttctcca ttttgagtca gttgaaaagg
    12541 aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga agctggctgg
    12601 tggctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca ctcgcgatca
    12661 tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct
    12721 cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt tacggaatta
    12781 tcgcctttac gctaatcgct gcactggggc gcgtgggcgt acaaacagcg tcagtcattg
    12841 ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca ctgtctaacc
    12901 tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggtgaa tatgttgacc
    12961 tcggcggcgt agccggcact gtgctgagtg tgcagatttt ctccaccacc atgcgtactg
    13021 cagacggtaa aattatcgtt attccgaatg gtaaaattat tgccgggaat attattaact
    13081 tctcccgcga gccagctcgc cgtaacgaat ttattattgg cgtggcgtat gattccgata
    13141 tcgatcaggt taagcagatc ctgaccgata ttatccagtc tgaagatcgc attttgaaag
    13201 atcgcgaaat gactgtgcgt ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc
    13261 gagtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta
    13321 aacgtgaatt tgatgccgcc ggtatcagct tcccgtatcc gcaaatggat gtgaacttta
    13381 agcgggtgaa agaagacaaa gctgcgtaat caacgctgag gccagataat actctctggc
    13441 ctctctctta ttagtttttc tgattgccaa ttaatattat caatttccgc taataacaat
    13501 cccgcgatat agtctctgca tcagatactt aattcggaat atctaacgtg ttttcttatt
    13561 actttcaagg tcttgcactt ggggcggcta tgatcctacc gctcggtcca caaaatgctt
    13621 ttgtgatgaa tcagggcatt cgtcgtcagt accacattat gattgcctta ctttgtgcta
    13681 tcagcgattt ggtcctgatt tgtgcgggga tttttggtgg cagcgcgtta ttgatgcagt
    13741 cgccgtggtt gctggcgctg gtcacctggg gcggcgtagc cttcttgctg tggtatggtt
    13801 ttggcgcttt taaaacagca atgagcagta acattgagtt agccagtgcc gaagtcctga
    13861 aacaaggcag atggaaaatt atcgccacca tgttggcagt gacctggctg aatccgcatg
    13921 tttacctgga tacttttgtt gtgctgggca gccttggcgg gcaacttgat gtggaaccaa
    13981 aacgttggtt tgcgctcggg acaattagcg cctctttcct gtggttcttt ggtctggcta
    14041 ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc
    14101 tggtagtggg atgtgttatg tggtttattg ccttgcagct ggcgagagac ggtattgctc
    14161 atgcacaagc cttgttcagt taggcgctat ctgatggaaa aataaaacag aggcgctaag
    14221 cttgcctcca gaggtcctga attttgggca agtcaattaa cacggagaga ctaacgtgaa
    14281 gttcaaagtt atcgccctgg cggcattaat gggtattagc gggatggcag cgcaggctaa
    14341 cgaattgccg gatggaccgc atattgtcac ctccggtacg gcaagcgtgg atgcggtgcc
    14401 agacattgcc actcttgcga ttgaagttaa cgtggccgcg aaggatgccg ctactgccaa
    14461 gaaacaggca gatgagcgcg tcgcacaata catttccttc cttgagctca atcagatcgc
    14521 gaaaaaagat atcagctcag cgaacttacg cactcagcca gattatgatt atcaggatgg
    14581 taaaagtatc ctgaaaggct accgcgcagt gagaacggtg gaagtcacgc tccgtcagtt
    14641 agacaaactg aattccttgc tggacggcgc gctaaaggcg ggtcttaacg aaattcgttc
    14701 tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac aaagcgcgta aggcagcgat
    14761 tgataacgcg attcatcagg cacaggaact ggcgaatggc tttaatcgta aactggggcc
    14821 ggtatatagc gtgcgctacc atgtttccaa ctatcagccc agcccaatgg tgcggatgat
    14881 gaaagctgat gctgcaccgg tgtccgcaca ggaaacttac gagcaggccg ctattcagtt
    14941 tgatgatcag gtcgatgtag tcttccagtt agaacctgtg gatcaacaac ctgctaaaac
    15001 acctgcagca caataatact tgtagcccga cgtattcgtc gggctaatgc ctttacccga
    15061 tattgcctcc tttttctgta attttctgta aaaccatgac gtaatcacct atctttgctc
    15121 gcggtttcat caaggatagg ctgactgaaa cggttaagcg gacaaggatg cgggagaggg
    15181 gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat
    15241 aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg caaaagcatt
    15301 gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc
    15361 ctcaataaag ccggacagga actgtatcaa aaactgttcc cggtttatca acgactttct
    15421 gcgattgata atgaaatcca taactcagga cgtcgttaac gagaaattgt gataggcata
    15481 gataatactt atccgacaat tatttttgat cagttgatta gtctcggaga taagtacgaa
    15541 ggcgtgacca tccagccagt tgagttcagc gaaaatggcg ttatcgataa tctttttgac
    15601 cgtcagttag attttattat ctccccacag catgtatctg cacgagttca ggaactggaa
    15661 aacctgacta tcagtgaact accaccgctg cgattgggct ttctcgtttc tcgccgttat
    15721 gaagggcgac aggagcaaga gctgcttcag gagctgccct agctacaaat gcgctttcaa
    15781 aaccgggcta acttcgaagc aatgattgat gcgaatatgc gtccttgtgg gatcaatcca
    15841 accattattt atcgtcccta tagttttatg gctaaaatca gcgccgtgga gcggggacat
    15901 tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat
    15961 tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat
    16021 cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    16081 cccagcatcg atccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    16141 tggtgaatat gtccgccgga cgcattttcc agatatcgat gcagataatc ccgatacatg
    16201 ggatgtgcac aatgatcaat ggtggtgcgg ataacgttgt tatacggatc ctgtgcgcca
    16261 aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc gacgttcata
    16321 aacagatcca tgccaatgcc gaagaaaaac gacaggcgag gggcgaagtc atcaattttc
    16381 agcccggcgg agattgctgc tttgatgtac tcaatcctat cggcgagcgt aaatgctacc
    16441 tgctgcacgc agttggcacc cgcttcaccc atgtggtaac cgctgatact gatggtatta
    16501 aatcgcggca tgttgccgga acaccaggcg atgatgtctg cgataatacg cattgacggt
    16561 tttggtgggt aaatataggt gttgcggcaa agatactctt tgaggatatc gttttgaatg
    16621 gtgccggtca gtttatcgga tgtaacacct tgctcttctg cggcgacgat ataaaacgcc
    16681 agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc cagcgggatc
    16741 tggtcgaaca ggacttttat atcttccacg gtgtcgatag cgacgcccgc tttgccgacg
    16801 tcgcccgcca cgcgtgggtt atcggagtcg tagcctcggt gggtggccag gttacggcga
    16861 taaaaagcgt tggactcttt tgctgtcgag aaaccagcat actgacggat ggtccacggt
    16921 tgggcggtat acatagtggc acgcgggcca cgaacgtagg ggggcaaacc aggaagggta
    16981 cccgtcacct ccagattatc gagatcggct tcggtataca gcggcttgat ggcgatccct
    17041 tccgcggttt gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg
    17101 gcaagctgtt gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat
    17161 gagggtattt gcgcttacgg tgagcttttt acgtgggttt gtcgccagca aaaaaatgca
    17221 ttcagacgtc gttttttgcc gcctgagatt tgtctatttt gcgatgtgat tcaaggaata
    17281 caaaaagaag tgaggataac gcctgatatg tgctatcagg cgttatttga tggattaatc
    17341 ctgacgaaga actttgtgac cataatcgag caacgcatca gtgactttac gcatcatgcg
    17401 gctttcagga gcaaagcggt gccagtagag catccggcgt tgaaatagcc ctggcgttaa
    17461 gtcaatcagt tcgccgctgg ccagctcttt ctcgatttgc aggtgcggga tcatacagca
    17521 ggtggtgccc tgacgagcaa gttgcacgaa cgcttccgaa gaattaacga tatggcaggg
    17581 tacgctgcca ggaggcagat cgaaattttg ctgcaaaaag gcctggtgca tatcgtcaag
    17641 atggtcaaac gcgaccactg gcgctttcag taatgccgaa cgcgttacgc cgttagggaa
    17701 atatttttcg gcaaatggtt ttgagctgac gaacagatag tcgagcgcac cgagtttatc
    17761 gacaagacaa ctcggcagcg cctgatgttg aatactcact gcgccgacca cttcgccgcg
    17821 acgcagacgt tcctgagtgc gggtttcatc ttctacctgc aagttgaggc ggatgggcga
    17881 atcagccaac acaggagcca gtgccggaag caaccacgtc gccagactgt cggcgttgac
    17941 cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc actcttcttc
    18001 cagcagctcc acctggcgta gtagcgccaa cagtttttgc ccttgttccg tcgggcgcgg
    18061 cggtacggta cgcactaaca acggctgccc gaacatattt tccagttgct taatgcgctg
    18121 tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg
    18181 tatcaccgca tccagtgcct gtaatgttct gtagtccggg cgtttcattg ctgttgtctg
    18241 ctcctggtaa atgctttttc ctgcactatg acacaatttt gtgtcgggtt gcatataccg
    18301 aagccgttgg cgggtaacat ttgtatcagt ggttaacgcg cagcttaaaa gatcagagat
    18361 ttgcgctaat gcatttcgct cagcgagttc gcgccctggt tgtgttgaat ggcgtggcgt
    18421 tattgcctca gtttgcctgt aaacaggggc ttgcgaacgg ggaactggtg cgcctgttcg
    18481 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaagggatgc
    18541 ccgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
    18601 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    18661 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
    18721 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcact cggtacaatg
    18781 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    18841 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    18901 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    18961 gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta ttgcagacgc ttccaagcag
    19021 gttgatattc tgggcaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    19081 gtggcgcgcc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtagtgacc
    19141 gataacggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    19201 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gtttgtttgc taaccgtggc
    19261 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ctattgtgaa atgatctgac
    19321 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt
    19381 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
    19441 aaatactttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    19501 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
    19561 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    19621 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    19681 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    19741 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
    19801 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    19861 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
    19921 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcatcgtggc
    19981 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    20041 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    20101 tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt acagcatctt
    20161 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtcaacc
    20221 aaaaatatga tgggcgcgaa agagatttcg ctaatgaaac ccggctcgct gctgattaat
    20281 gcttcgcgcg gtactgtggt ggatattcca gcgctgtgtg acgcgctggc gagcaaacat
    20341 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    20401 acctctccgc tgtgtgaatt cgacaatgtc cttctgacgc cacacattgg cggttcgact
    20461 caggaagcgc aggagaatat tggcctggaa gttgcgggta aattgatcaa gtattctgac
    20521 aatggttcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcacggtggt
    20581 cgtcgtctga tgcacatcca cgaaaaccgt ccgggtgtgc tgactgcgct caacaaaatt
    20641 tttgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc ccagatgggt
    20701 tatgtagtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    20761 aaagctattc cgggtaccat tcgcgcccgt ctgctatact aaatcccttt ctctgaaaat
    20821 caacgggcag gtcactgact tgcccgttat tttatccctt ctccacaccg ttgccgcgtt
    20881 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    20941 gtttatttaa tggggattta cgatggcggg actgtacagg gctgtaacgg taaagccctc
    21001 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    21061 ctgttactca taagtttcag cgcttattaa cagtcagtca caggggagga gcaatccccc
    21121 cttaccctta ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg
    21181 tgtgtcgccc tcaccactcc cagactttcg acggtgtaac caccgcaggc agtgggatat
    21241 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    21301 gcattttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    21361 ggcgctgacc gtactcatca aaggcgacca gcggtgtaat caacacgtct aatcgggaaa
    21421 ggggtagcac gtcacgcaca tccagttttg gctcatggat cttcaacttg ttcatcacca
    21481 gttcgctttg tggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    21541 ctggaagata tacgcgctta ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga
    21601 gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc gggggataag
    21661 tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac
    21721 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    21781 gtaattgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    21841 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    21901 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    21961 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    22021 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    22081 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    22141 cgcgtagtca cgagtcttcg ctttttcttg cgctaactca tagctgatat tcagtgcggc
    22201 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    22261 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    22321 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    22381 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    22441 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    22501 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    22561 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgctgaga
    22621 tgcatggttt aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctgccgc
    22681 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc
    22741 gcaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    22801 atctacctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcgctg gctggttggg
    22861 tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggacaaa gtgaccggcg
    22921 aaaccggtga agccatcgac gatctgcgta acatcgcgca gttgggttac gacgaagacg
    22981 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgagtacgtc cgtgttgccg
    23041 cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac
    23101 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgatat atcccggcaa
    23161 gaatttcagc gtcgccgtca ggccctggta gagcaaatgc aacccggcag cgccgcgctg
    23221 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    23281 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa
    23341 agcgatgaca ctcataacca cagcgttttg tttaaccgcg ttcgcgacct gacggcagag
    23401 atctggtttg gtcgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc
    23461 gcactggcat tcagcgaaat caatcaacaa ctttatcaac tacttaatgg tctggatgtg
    23521 gtttaccacg cccagggcga atatgcatat gctgatgaaa tcgtgaacag cgcgcttgag
    23581 aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt
    23641 cctgttgttc atgaaatgcg cctgttcaaa tcaccagaag agattgccgt acttcgccgc
    23701 gcgggtgaaa tcaccgcact ggcgcataca cgggcgatgg aaaaatgccg tccggggatg
    23761 ttcgagtacc atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgttac
    23821 ccttcctata acaccattgt tggcagcggt gaaaacggct gcattttgca ttacaccgaa
    23881 aacgagtgtg aactgcgtga cggtgacctg gtgttgattg acgcgggttg tgaatacaaa
    23941 ggttacgctg gcgacattac ccgcaccttc ccggtcaacg gcaaattcac tcaggctcag
    24001 cgtgaaatct atgacattgt gctggagtct cttgaaacca gcctgcgcct gtatcgtccg
    24061 ggaacttcca ttcaggaagt cactggtgaa gtggtgcgca tcatggttag cggtctggta
    24121 aaactcggca tcctgaaagg tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct
    24181 ttctttatgc atggccttag ccactggtta ggactggatg ttcatgacgt tggtgtttat
    24241 ggtcaggatc gctcgcgtat tctggaaccg ggtatggtac tgaccgtaga accaggactg
    24301 tatattgcgc cggatgcaga agtgccagaa cagtatcgcg gtatcggcat tcgtattgaa
    24361 gacgatattg tgattaccga aaccggtaat gaaaacctca ccgccagcgt ggtgaaaaag
    24421 ccggaagaaa tcgaagcgtt gatggcagct gcgagaaagc aatgagcgta atcatcgtcg
    24481 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    24541 tgccagtaca tttgattgaa gcgaccgcgc cagagtcaca tgcgcatccg ggctttgatg
    24601 gacgagctat tgcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc
    24661 aatctctggc tgattgcgca actgccatca ccaccgtgca tgttagcgat cgtggtcacg
    24721 ctggatttgt caccctcgcc gcagaagatt accaactggc ggcgctgggg caggttgtcg
    24781 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg caaagcacct ggcgtaacgc
    24841 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagccacgtt gaagtgacgc
    24901 tggagagtgg cgagacgctg acgggccgcg tgctggtagc cgctgatggc acccattcag
    24961 cgttagccac cgcgtgcggt gttgactggc agcaggagcc ttacgaacaa ctggctgtga
    25021 ttgccaacgt tgcgacttcc gttgcgcatg aagggcgcgc ttttgaacgc tttacacaat
    25081 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    25141 cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga gaagttctgc cgtgaactcc
    25201 agtcggcctt tggctggcga ctggggcaaa ttacccacgc cggaaaacgt agtgcttatc
    25261 cgctggcgtt aacccgcgcc gccaaaccta ttacccatcg taccgtgctg gtggggaatg
    25321 cggcgcaaac tctgcatccc atcgccgggc aaggatttaa cctcggtatg cgtgatgtga
    25381 tgagtcttgc agaaaccctg actcaggcgc aggaacgcag agaagacatg ggtgattacg
    25441 gcgtattgtg ccgttatcag cagcgtcgac agcgcgatcg cgaagcaacc atcggcgtca
    25501 cggacagcct tgtacatctt tttgccaacc gttggacgcc gctggttgtc gggcgcaaca
    25561 tcgggctgat gacgatggaa ttatttaccc cggcacgcga tgtgctggcg cagcgcactc
    25621 ttggttgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta
    25681 gccattgttg gcggcggcat ggtcgggctg gcggttgcct gcggcttaca gggtatcggc
    25741 ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc tggcggcgga tgcaccacca
    25801 caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc
    25861 tggcaggaca ttctctctcg tagggcctgt tgctatcacg gcatggaagt gtgggacaaa
    25921 gacagctttg gtcacatctc gtttgacgat caaagcatgg gctatagcca tcttgggcat
    25981 atcgttgaaa actcagtgat tcactacgcg ctgtggaaca aagcgctgca gtcgtcagat
    26041 atcactctgt tggcccccgc agaattacag caggtcgcct ggggtgaaaa tgaaaccttc
    26101 ctgacgttga aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggcgct
    26161 aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta tcagcatcac
    26221 gcgctggtgg cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg acaggttttt
    26281 catggcgaag gcattctggc ctttttgccg cttagcgatc cgcatctttg ctcgattgtc
    26341 tggtcactgt cgccagagga agcgcagcgg atgcagcagg caggcgaaga cgaatttaat
    26401 cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga aagtgagcgc
    26461 ctggtgttcc cgctaacggg gcgttatgcg cgccagtttg ccgcgcaccg tctggcgctg
    26521 gtgggcgacg ccgcgcatac cattcacccg ttggcggggc agggggtaaa tctcggcttt
    26581 atggatgctg cagagctgat tgctgaactg aaacggttgc atcgtcaggg taaagacatc
    26641 gggcagtaca tttacctgcg tcgctatgag cgtagccgca agcacagcgc ggcgctgatg
    26701 ctggctggta tgcagggatt ccgcgatctg ttttccgggg ctaatccggc gaaaaaattg
    26761 ctgcgtgata ttggtctgaa actggctgat acgcttcctg gcgttaagcc acaacttatt
    26821 cgccaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcttctgc
    26881 tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc
    26941 atttgaaata aactaa
//
LOCUS       DACADA010000056        26821 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.057, whole genome shotgun sequence.
ACCESSION   DACADA010000056 DACADA010000000
VERSION     DACADA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.057"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..848
                     /locus_tag="ISB17_002267"
     CDS             <1..848
                     /locus_tag="ISB17_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP1980197.1"
                     /translation="QKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRGKYLLQAIR
                     DVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQFMNSRLPV
                     ATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLLNPDQLPMM
                     GFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLRDTTIKVRK
                     EESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPANIEMAIRL
                     VGSYY"
     gene            complement(991..2355)
                     /locus_tag="ISB17_002268"
     CDS             complement(991..2355)
                     /locus_tag="ISB17_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAP1980198.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2444..3220)
                     /locus_tag="ISB17_002269"
     CDS             complement(2444..3220)
                     /locus_tag="ISB17_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP1980199.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            complement(3225..3863)
                     /locus_tag="ISB17_002270"
     CDS             complement(3225..3863)
                     /locus_tag="ISB17_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP1980200.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(3860..5122)
                     /locus_tag="ISB17_002271"
     CDS             complement(3860..5122)
                     /locus_tag="ISB17_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HAP1980201.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5119..6027)
                     /locus_tag="ISB17_002272"
     CDS             complement(5119..6027)
                     /locus_tag="ISB17_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1980202.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6193..6990
                     /locus_tag="ISB17_002273"
     CDS             6193..6990
                     /locus_tag="ISB17_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1980203.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GRCIYLDAGTTTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(7041..7697)
                     /gene="pphB"
                     /locus_tag="ISB17_002274"
     CDS             complement(7041..7697)
                     /gene="pphB"
                     /locus_tag="ISB17_002274"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP1980204.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(7803..10364)
                     /gene="mutS"
                     /locus_tag="ISB17_002275"
     CDS             complement(7803..10364)
                     /gene="mutS"
                     /locus_tag="ISB17_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP1980205.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            10650..10994
                     /locus_tag="ISB17_002276"
     CDS             10650..10994
                     /locus_tag="ISB17_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAP1980206.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(11033..11461)
                     /locus_tag="ISB17_002277"
     CDS             complement(11033..11461)
                     /locus_tag="ISB17_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP1980207.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(11506..13584)
                     /gene="flhA"
                     /locus_tag="ISB17_002278"
     CDS             complement(11506..13584)
                     /gene="flhA"
                     /locus_tag="ISB17_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAP1980208.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(13658..14668)
                     /gene="hypE"
                     /locus_tag="ISB17_002279"
     CDS             complement(13658..14668)
                     /gene="hypE"
                     /locus_tag="ISB17_002279"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAP1980209.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(14665..15786)
                     /gene="hypD"
                     /locus_tag="ISB17_002280"
     CDS             complement(14665..15786)
                     /gene="hypD"
                     /locus_tag="ISB17_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAP1980210.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(15786..16058)
                     /gene="hypC"
                     /locus_tag="ISB17_002281"
     CDS             complement(15786..16058)
                     /gene="hypC"
                     /locus_tag="ISB17_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAP1980211.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(16049..16921)
                     /gene="hypB"
                     /locus_tag="ISB17_002282"
     CDS             complement(16049..16921)
                     /gene="hypB"
                     /locus_tag="ISB17_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAP1980212.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(16925..17275)
                     /gene="hypA"
                     /locus_tag="ISB17_002283"
     CDS             complement(16925..17275)
                     /gene="hypA"
                     /locus_tag="ISB17_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP1980213.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            17487..17948
                     /gene="hycA"
                     /locus_tag="ISB17_002284"
     CDS             17487..17948
                     /gene="hycA"
                     /locus_tag="ISB17_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAP1980214.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            18073..18684
                     /locus_tag="ISB17_002285"
     CDS             18073..18684
                     /locus_tag="ISB17_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1980215.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            18681..20507
                     /gene="hycC"
                     /locus_tag="ISB17_002286"
     CDS             18681..20507
                     /gene="hycC"
                     /locus_tag="ISB17_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAP1980216.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            20510..21433
                     /gene="hycD"
                     /locus_tag="ISB17_002287"
     CDS             20510..21433
                     /gene="hycD"
                     /locus_tag="ISB17_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAP1980217.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            21451..23160
                     /locus_tag="ISB17_002288"
     CDS             21451..23160
                     /locus_tag="ISB17_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAP1980218.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            23170..23712
                     /gene="hycF"
                     /locus_tag="ISB17_002289"
     CDS             23170..23712
                     /gene="hycF"
                     /locus_tag="ISB17_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAP1980219.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            23712..24479
                     /gene="hycG"
                     /locus_tag="ISB17_002290"
     CDS             23712..24479
                     /gene="hycG"
                     /locus_tag="ISB17_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAP1980220.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            24476..24886
                     /locus_tag="ISB17_002291"
     CDS             24476..24886
                     /locus_tag="ISB17_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAP1980221.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            24879..25346
                     /gene="hycI"
                     /locus_tag="ISB17_002292"
     CDS             24879..25346
                     /gene="hycI"
                     /locus_tag="ISB17_002292"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAP1980222.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            25371..25556
                     /locus_tag="ISB17_002293"
     CDS             25371..25556
                     /locus_tag="ISB17_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980223.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            25582..26001
                     /locus_tag="ISB17_002294"
     CDS             25582..26001
                     /locus_tag="ISB17_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1980224.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(26093..26530)
                     /locus_tag="ISB17_002295"
     CDS             complement(26093..26530)
                     /locus_tag="ISB17_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980225.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
BASE COUNT         6211 a         7035 c         7081 g         6494 t
ORIGIN      
        1 tacaaaaaat tggcgagaga acttattatg ttcaacgcta tttttattcc actacgtttt
       61 atgtcggaga caaaggggtt ttgctgtttg atgctccgga gggtcgcggt aaatatttat
      121 tacaggcaat tcgtgatgtg actccattac ccgtaacagc gctggtttat tctcattacc
      181 atgtggatca cattggcgat agtccatttt ggaacgatga ggcgaagaaa gaaggcgtga
      241 atctgcgtat tattgccagt aaagccactg ctgaaaaaat gcaatttatg aacagtcgct
      301 tacccgtcgc cactcaggtt ttaagtaaaa aagatgacca gtttaaattt gaaaagcaga
      361 ccattgaatt acaccgattt gttaaagcag gacataccga cgaccattct gtatggttgc
      421 taaaacagga aaaagtagct cacagcccgg atctcctgaa tcctgaccaa ctgccgatga
      481 tgggcttcgc cgtatcggat acgttggttt accatgacag taatttgcgt caggttgaaa
      541 tgcttgactg gaaatatttt attggcgggc atggtaatat tggttcacac gatgatttta
      601 aatttcaacg ccagtttctt aatgatcttc gggatacgac aattaaagtc agaaaagaag
      661 aaagttttgg taaatttatg aataaaacgg caaataatca tgctgatttt gcgcgagccc
      721 agcgcgaagc aattattaaa aaagtaactg aagtattacg tccgaaatat ggccatatgt
      781 atggctacga cgcatctatg cctgctaata tcgaaatggc aataaggttg gtcggttctt
      841 attactaaga taaaatcaac ttgcaagaga ctgtaattat attgcaaagt gaaaattaac
      901 ctcggtagcg agatattacc gaggttaata ttggcataca ttacaccctt atcaggcact
      961 taaacgatac atcatatctt atctggatta ttagatgacg agccatacgc accaggtaat
     1021 taagaatccg gtaaaaccga gaatggtcgt taacactgtc caggttttca gaccgtctgc
     1081 taccgacaac cccagatatt tggtcacaat ccagaaccct gagtcattaa tatgtgacgc
     1141 gccaagccca ccaaagcagg ctgccagcgt caccaatacg cactgaattg gattcaatcc
     1201 catcaccgct tctgagagta acccgccggt tgtcaggatt gctacggttg ctgacccctg
     1261 cgatgcacgc agcgccagtg aaataataaa tgcggctggt aacagaggca ggtcaatcat
     1321 ttgtaacatg ttggcaaggg ctttaccgac gcccgattcc accagcactt tgccaaatac
     1381 ccctccagca ccagtaacca aaatcactac cgccgcagtg ggaagggcag agcccataat
     1441 gtcgctggtg tgttgtaagc tccagccgcg acgtaaagcc aataaccaga atgccagcac
     1501 cagcgcaatc attagagcta ccattggtga gccgatcaac tgtagcgtac caagcagggg
     1561 atgcgaaggc ggcatcagtg ttgcagaaac cgttcccgcc atgataatcg cgataggaat
     1621 aacaattagc gaggtgacta acgcgacgcc cggtggattt attttatcgc ttaattttgt
     1681 cgcgccttcc tcactggccg gagccagttg catctgttcc agtacctcga ccgacatcgc
     1741 atattggcgc ttattgatta ttttcgctgc aaagtagcca acaatcccta cgggaataga
     1801 aatcgcaata ccgatgatgg ttagccagcc gatgtctgcg tggagtaatc ccgctgcggc
     1861 gacagggcct ggatgcggcg gtaccgccac gtgaacagtg agcatgatcc cagcgacagg
     1921 caggccaaat ttgagtggtg atattttggc aaccttggca aaaccgtaaa tgattggcgc
     1981 aagaataata aagccgacat caaagaagac gggaatgccg aggaagaacg ctgccagagt
     2041 cagcgcagcg atagttcgtt tgtcacctaa cttgcgactg aaataattag ccagtgactc
     2101 tgcaccacca gagtgttcga tcatacgccc cagcatagcg ccaagaccaa taataatagt
     2161 gacggaacca agcacaccgc ccatcccggc gatcatcact ttacccactt cgcccgccgg
     2221 tatacccgcc gcaagtgcga ctaacaggct gacgaggagc agagcaacga atggttgtac
     2281 ctttgccttg atgaccagta gcaacagcat gattacgcca gctaacgcaa tgcataacaa
     2341 tgtaattgtg gacatgggaa accctgtctg aaagttatag ttaacctacc ccatccgtag
     2401 atggggggat gtatgggtac gttgtaatta gggattgagc gaattagcgc caggcgtcaa
     2461 accagccaag cccttctttg gtgaggccac gaggtttgta ttcacaaccg atccagccct
     2521 gatatcccac ttcatcgaac aggcggaaca gccacggata gttgatttct ccatcgtccg
     2581 gttcatgtcg atcaggtagt ccggcaattt gtacgtgcgc atatttcccg gcgtagttgc
     2641 ggattaaatg tgtcaggttg ccatctactt tttgcgcatg gaaagtatct agttgaataa
     2701 acacgttatc tcgcgcaacc tcttcaacaa tagccagtgc ctgatactgg ctggagaaga
     2761 gataatgagg cttaacgccg gggctgagtg cttcaaccaa tattcgcttg ccgtgtggcg
     2821 caaagcggtc ggcagcgtag cggagattat caataaatac tgcccggtac cgttcagcat
     2881 cttcgccagc gggcacgacg cctgccatca catggacttg ttcacaattg agcgccaatg
     2941 catattccag tgccaggtcg atgtctgcgc gtgcttcgtg ctcacgtccg ggaagggcgg
     3001 ataatcccca ttctcccgca ttaatatctc cgggagcggt attgaacagc gccagtgtca
     3061 gatggttttg caccagttgc tgctggattt gctgggggga gtagtcatag ggaaacagaa
     3121 actccacagc atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt
     3181 cggtgaacat catggataaa ttagctgcaa aacgaggcat tgcattaact ccttaattcc
     3241 gcaatttcac ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt
     3301 gccgtttcct ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc
     3361 actgggccat gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc
     3421 tgtgcgatgc gtttatcgcc cggtcggtaa tagggcacca gcgggacgtt tcccatccgc
     3481 atcaccacgt atggtgtgaa cggacgaata acgttattgc tgtccagccc ttccaggcag
     3541 gaaagcgccg tcgaccacgt actgtgcaaa tgcaccaccg ctttacagag cggattgttg
     3601 cgatacagcg ccagatgaaa gagcacctct ttcgaggggt tgtcaccact taaccattcg
     3661 ccatccgcgg tgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc
     3721 ggtgtcgcca gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt
     3781 gcatatccgc gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc
     3841 tctacttttg cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt
     3901 catcaccgaa gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc
     3961 acggcacgcc gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg
     4021 tgactacgcc ggaggtctca ccgcctgcga caataaagcg tgtcacgcct tccgctgcta
     4081 accgcgccgc tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt
     4141 attgctgttg aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa
     4201 gtacactttc ctggcccaga acccactctg ccagctcgtg tgcataagcg gccagagttt
     4261 cggttgagag gcagcgtgcc acatcaacct cacgggctgg tgcaatttga cggtaatgtg
     4321 ccacctggcg gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga
     4381 gcggacgccc tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc
     4441 caatcgccag accagaaccg cccgttacca gtggggcatc gcgcaaggct tccccctgaa
     4501 tttccagatg gtgttcggtc agcgcatcaa gcaccgcgta gcgatagccc tcttgctgta
     4561 agcgagccag ctcctgacga acggctttca caccttgttc gaaaacatgt gctgaaacga
     4621 cgccgcagcg ccctgtggat tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg
     4681 gatttaccgg gtgatggcgc attccggatt cggccagcag ttgattcatt acgaacaaat
     4741 acccctgata aaccgtacgt ccgttgactg gcagggccgg agagaagacc gtaaacggtg
     4801 tgtcgagagc atccattaat gcatcggtaa ctgggccgat attccctttc gccgtactgt
     4861 cgaaagtaga gcagtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca
     4921 gagccgccag cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga
     4981 tcaccagtgc gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg
     5041 ttggtagacc gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcag
     5101 cgataacgcc aatcttgatc atgatttcgc tcccggtagg gtgatgccag agaaaatctt
     5161 gataactgcg ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt
     5221 caatgctgtt gaggccaatg gcagcgggaa gtgcagggct ttggcggtat cggcaaccag
     5281 accaagatcc ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac
     5341 atgacgcatc cggttttcga acatccatga atttccggcg gcattggtca cgacgtcata
     5401 catcacatcc agcgggatcc ccgcacgggc tgcaagcgcc atcgcttcgg ctccggcagc
     5461 aatatgtacg cccgccaaca actggtggat aatttttacg gtcgaaccca gtcccggttc
     5521 tgctccgatg cgataaactt ttccggcaac ggcttccagc acgggtgcca gtcgttcaaa
     5581 ggcaatatcg ctaccggagg ccatgacagt catttcaccg ttagcggctt ttaccgcacc
     5641 accagaaacc ggcgcatcca gcatttccag accgaatcca gccagagcgg tagcaatttc
     5701 ttgcgcatca gcattggcga tagtggaaga aaccattact gccgtaccgg gtttcagatg
     5761 ttgtgcaacg cctttttcac caaacagcac ctgtttaacc tgggtcgcat tgaccaccag
     5821 caccagcaga gcgtccagtt tttcggcaaa cgtcgcggcg ttatcagaaa ccccgcaagc
     5881 accagcctct ttcaacgtag cgcaggcatt gctgttcagg tctgcgcccc aggtagaaag
     5941 acctgcgcgg acacatgaca gtgctgctcc cattcccatt gaccctaagc caacgatacc
     6001 gacatgaaac tccgatcccg ttttcatctg ctctccttgt taatttaagt gatattttgt
     6061 ttgatattgt gaatataagc gcggaaagat aacgatatgg tgagctgatt cacaaaaatt
     6121 aacattgtgt gttattttat gtgaactaag cgttagttgc gccgcgcacg tttcgcaggc
     6181 aaatagcgta gaatgtcagc aggacaaagg aaggagcaaa agttgatacc cgtagagcgt
     6241 cgccaaatca tccttgagat ggtagctgaa aaaggcattg ttagtattgc tgaactaacg
     6301 gacagaatga atgtgtcaca tatgaccatt cgtcgggatt tacaaaaact ggagcagcag
     6361 ggagccgttg tgctggtgtc cgggggcgtc cagtctccgg gacgcgtggc gcatgaacct
     6421 tctcatcagg taaaaactgc gctggcaatg acgcaaaaag cggctattgg caagctggcg
     6481 gcaagtcttg ttcagccggg acgttgtatc tatctggatg cgggaacgac cacgttagcg
     6541 attgcacagc atctgattca catggagcca ctcacagtgg ttactaacga cttcgttatt
     6601 gcggactact tgctcgacaa cagtaattgc acaattattc acactggcgg cgcagtgtgt
     6661 cgggaaaacc gttcctgtgt tggggaagcc gctgcgacca tgctgcgtag cctgatgatt
     6721 gatcaggctt ttatttctgc atcgtcatgg agtgtgcggg ggatctctac gccagcagaa
     6781 gataaagtca cggtgaaacg ggcgattgcc agtgccagcc gccagcgagt tttggtctgt
     6841 gatgcgacga aatatggaca ggtggcgaca tggctggcgt taccattaag cgagtttgat
     6901 cagatcatta ctgacgacgg tctgccggag agtgccagcc gcgcgctggc gaagctggat
     6961 ctctctttgc tggtagcgaa aaatgaataa tgacctgcaa taacgccggg ttactcatgc
     7021 ttcacagaag aagcatgaga ctactttatt ttataaaatg acagccgccc gcttttcggc
     7081 gatccggtat ctatataaat ctggttagcg aacgtctgaa tgttatcaaa catcatatga
     7141 ccaaatataa agaagtcggc gccgtttatt ttttgtaatt cgctattaag tgatttctgc
     7201 acacgatcaa caggccagag taattcgctc tccgctattt ctttgccaaa ctgatactcc
     7261 ttccccggat aatctgcatg tgcgatgaca tattttatgt tgtcgttagt gatttcaata
     7321 atatgtggaa ggagatggaa tttcagcaac agatctattg cctcttgttg ctctgaatca
     7381 tttaaatcga aaaaccagtc accaccgctg gcaagccaca tatttccatc gccagtttcg
     7441 aatgcctcaa gcgccatcgc ttcgtggttg cctttgacgg agataaacca gggctggttt
     7501 aacaggcgca gcacgttaag actctccggc ccacgatcga tgttatcgcc taccgaaata
     7561 agtaagtcgg tttcaggaca aaaagagagt tgatgtaagc gggattgtaa taattgatag
     7621 tcaccatgaa tatcaccaac ggcccatata tggcgatagt gatgggcatt gattttttga
     7681 tagcgtgtag atggcatggt tttaccctgt aaaataagtt ttcctattat acagggtatt
     7741 tttatttgat tcgtcagttg tcgttaatat tcccgatagc aaaaggctat cgggaattat
     7801 tattacacca gactcttcaa gcgataaatc cattccagcg cctgacgcgg agtcagtgaa
     7861 tccgggtcga ggttttccag tgcctcgacc gcaggcgaag tttcttccgg tacggacagc
     7921 aaagacattt gtgtaccatc cacttgcgta gcagcggcgt tcggcgaaat gctttccagc
     7981 tcacgcagtt tttgccgtgc gcgcttaata acctcttttg gcacacccgc cagagctgca
     8041 accgccaggc cgtagctttt gctcgccgcg ccatcctgca cgctatgcat aaaggcaatg
     8101 gtgtcgccgt gctccagtgc atcgagatgc acgttggcga cgccttccat tttctccggt
     8161 aactgggtca gctcgaaata gtgggtagca aacagcgtca acgctttgat cttatttgcc
     8221 agattttccg cacacgccca cgccagcgac agaccatcgt aagtggacgt tccacgcccg
     8281 atctcatcca tcaacaccag actatattcg gtggcgttat gtaaaatatt ggcggtttcg
     8341 gtcatctcca ccataaaggt tgaacgcccg gaagccagat catccgcagc acctacgcgg
     8401 gtaaagatac ggtcaatcgg gccaatctcg actttttgcg ccggtacgta gctgccgata
     8461 tacgccatca gcgcaatcaa cgcggtctgg cgcatatagg tacttttacc gcccatgttc
     8521 ggaccggtaa tgatcaacat gcgacgctgc ggcgacaggt tcagcgggtt agcgataaac
     8581 ggctcattca gtacttgttc aactaccgga tggcgacctt cggtaatacg aatgccaggt
     8641 ttatcaatga aggtcgggca ggtatagttc agggtatagg cccgttccgc caggttcacc
     8701 agcacgtcga gttccgccag cgcgctcgcg ctctgttgca acgcttccag atgcggcaac
     8761 agcaggtcga acagctcttc ataaagttgt ttttccagag ccagtgcttt gccttttgag
     8821 gtgagaactt tatcttcgta ctcttttagc tctggaatga tgtagcgctc ggcgtttttc
     8881 agcgtctggc gacgcatgta gttgattggt gccaaatggc tttgcccacg gctgatttga
     8941 atgtagtagc cgtgcaccgc attaaagcca actttcagcg tgtccaggcc ggtacgttca
     9001 cgctcgcgga cttccagacg ctccagataa tcggtcgcgc cgtcagccag cgcgcgccac
     9061 tcatccagct cttcgttata gcccgatgcg ataacaccac cgtcgcgtac cagcaccggt
     9121 ggtgtgtcga tgattgctcg ctccagcaga tcgcgcagct cggcaaactc gcccatcttc
     9181 tcacgcagcg cttgtaccgg tgcactatcg acattttcta actgcgcacg caactccggc
     9241 agttgctgga aagcgtgacg catacgggcc agatcgcgtg ggcgagcggt acgcaacgcc
     9301 agacgcgcca gaatacgttc caggtcgccc acctgacgca gtaccggctg cagctcggcg
     9361 gtgaaatcct gcaatgcgcc aatagtttgc tgacgctcaa gcaacacgcg ggtatcgcgc
     9421 actggcatat gcagccagcg tttcaacata cgactaccca tcggcgttac agtgcagtcg
     9481 agcactgaag ccagcgtatt ttccgcaccg ccggccaggt tctgagtaat ttccaggtta
     9541 cgacgcgtcg cggcatccat aatgatgctg tcctgctgac gttccatagt gatagaacgg
     9601 atatgcggca gggtcgtgcg ttgggtatct ttcgcatact gcaacagaca accggcagca
     9661 caaagtccgc gtggtgcgtt ctccacacca aaaccgacca gatctcgggt gccaaattgc
     9721 aggttcaatt gctggcgagc ggtgtcgatt tcaaactccc acagcgggcg acgacgcagg
     9781 ccgcggcgac cttcaatcag cgacatctcg gcgaaatctt ctgcatacag taattccgct
     9841 ggattcgtac gttgcagttc tgccgccatc gtttcgcggt ccgccggttc gctcaggcga
     9901 aaacgaccgg aactgatatc cagcgttgcg tagccgaaac ctttactgtc ctgccagata
     9961 gccgccagca ggttgtcctg acgttcctgc aacagcgctt catcgctgat ggtgcccggc
    10021 gtaacgatac ggacaacttt gcgctcgacc ggccctttgc tggtcgccgg atcgccaatc
    10081 tgttcacaaa tagcgacgga ttcgccctga ttcaccagtt tggcgaggta gttttccacc
    10141 gcatggtagg gaatccccgc catcgggatt ggctctcccg ctgaagcacc gcgtttggtc
    10201 agtgaaatat ccagcagttg cgacgcccgt tttgcgtcgt cataaaacag ttcataaaag
    10261 tcgcccatcc ggtaaaacag caggatctcg ggatgctggg ctttcagctt gagatactgc
    10321 tgcatcatgg gcgtatgggc gtcgaaattt tctattgtac tcatggggtt aagtccggct
    10381 ccctgatgtt aaatgtagtg tgatggtttt tattttcgtt gtaatcacat aaggcgcaca
    10441 tgataccgga gttaatcatt tcaggggaag acgcgaaagg tgagttcttg atgtcggttc
    10501 cgcgaaaaat gctgctttgc agcttttgcg tgatgacgat gaaacaagat cgccaaattc
    10561 gtcggttgaa gaaacctcct ccttcagagc catagtcccc tttaaaatta agttgtaata
    10621 taaatgcacc ttaagagtat ggaggcagca tgtccggcaa gcgtatctct cgtgaaaaac
    10681 tgacgattaa aaaaatgatc gatctttatc aagcgaaatg cccgcaggcg tcagcagagc
    10741 cggagcatta cgaggcgttg tttgcttacg cgcaaaagcg gctggataaa tgtgtgttcg
    10801 gcgaagagaa accagcctgt aagcagtgtc cggtgcactg ttaccagcct gcaaagcgtg
    10861 aagagatgaa acagattatg cgttgggccg ggccgagaat gctgtggcgt catccgatct
    10921 taaccgtgcg tcatctcatc gatgacaagc gtccggtgcc ggagttgcca gaaaaatatc
    10981 gtccaaagaa ataaatctgc cggagagcca tactctccgg caaatacggc gcttacgctt
    11041 tggtggcaat caggatggct gatgctttga tcaatgcaat aacgcgcgag ccagtggtga
    11101 gtttcatttc atcctgactt tcatttgtta ccactgccac aatttcaaaa cccgcatccg
    11161 ttttgatatg gacagtggcg ttcactgcgc cttcggtgat tgtcgacacg ctgccagcaa
    11221 actggttgcg agcagagaat ttcagtccac agtcttccgt tgccagcgtt acccacgggg
    11281 ctttaatcag ggcaatcgct tctttccctt tcgccagacc cagcgcttgc tggctgctgt
    11341 gagtcacaat tgccaccagc tttgcaccac ctgccagcgt cagttctact tcgtcattca
    11401 ccgcgcccat tgctactgcg ctaactgttc cggttaattg atttcttgct gaaactgcca
    11461 tactgcctcc tttcattgaa tgttgatcga gtataaccac atctgttaaa tcaatgccga
    11521 tttatcaatt cccagtcgct tcattcgtga cagcagagtc gtgcgtttca gccccagacg
    11581 ttgcgcagcg cctttaggcc cggcaaccac gccgtttgtt tctttcagca cgcgaacaat
    11641 caactgatat tcatcttcgc cctcctgggc gacaaccgtt gcggcaggcg gcgtttcagg
    11701 ttctggtaaa gcaatatctg gcaatgacag ctgcagcacg ttgccgcgtg ttagcaatac
    11761 cgcgcgctcg atgacgtttt ccagctcacg cacgttgccc ggccattcca tgttgctcag
    11821 gatgcgcagc gtctcggcag gaatgctgtc gatattgcgc cccagacgac gggcaatttt
    11881 gaaggtaaag gctttcgcca gcagcggaat atcttccgga cgctcgcgta gtggcggcag
    11941 gtggatcggg aatacgttca ggcggtaata gagatcgcta cggaactctc ggtcggcgac
    12001 catttttttc agatcgcggt tagtcgcggc aattaaacgt acgtccgtct gaatgatttt
    12061 gttgctgccg agacgttcaa actcctgttc ctgtaataca cgcagcaact tcggctgtaa
    12121 ctccagcggc atatcgccca cttcatcgag gaatagtgag cttttatccg ccagttcaaa
    12181 acgaccaatg cgctgagcgc tggcaccggt aaaagccccg cgctcatgac caaatagatc
    12241 gctttccagc aatccggcag gcatcgccgc gcagttcatt ttgaccatgc ggcgattatt
    12301 acgcccactg agattatgga tcgcgcgggc aatcagctct ttacctgtac cagtttcacc
    12361 gaggatcaga acggtactgt cactttgcgc caccatttca acttgtttaa gtacgctgta
    12421 catggcttcg ctgcggccaa taatctcgcc aaattcacta tcaacattgt tgagctgctc
    12481 ggtcagggcg aggttttcat caaccagccg ttctttcaga cgatgaattt cctgataggc
    12541 gagggcgtta tcgacagcga ttgccacacg ttcggcaatc tggcgcagta aattcagatt
    12601 ggtagtggta aagactttct cttcgcattg cgccagtttc agtacgccca gcatggtgtc
    12661 gccagacatc agcggtaaca ggcacaaggt ttgaatctga ttgccccagg tgttgaacaa
    12721 catgcgttca tagggggcta aatcgtcccg ctcgtggaga ttaatcaaca gcatctcttt
    12781 acttttgaag acgcgttcgg tgagggttcc ggcttcatcg acttcgctct gttcgtgagc
    12841 gggatgctgt ttatcaagat agtgagtgga gtagatgttg agtttgttct tacggtggct
    12901 gcgtaagacg atgctgatat cgtcaatgtc gaagtagtag tggatttctt tggcgacttc
    12961 gctgaccagt tcgtccatat ccaggcggga aagcaccgcg ttagtgatgg cgaccaggat
    13021 gcggaagtta tcgcgttccc ggcataacaa ctcatagtcg acattgttaa cgacgcggct
    13081 ctggatttgt tcggtgacga cagaaacgat ctgcgtaaat gtttgcagac gattgaactc
    13141 tttttcgctc caggggcgat cgtcataacg aataaattca cagccaccaa aaatatgccc
    13201 ttccgccgcc agtggcatca ggcaatagtg accaaatttg gggtatagcc cacctgtggc
    13261 cagctgcggc caggtttcac aaaattcttc gtaactgcaa tgcagcgtat cagggcgcga
    13321 caaaatgctg cgtaccggac cgtgtgccag aacagtttcg tcttcatatt taatgggggt
    13381 gtctttttca cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat
    13441 cgccgcgttg tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga
    13501 ggccagatcg ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag
    13561 atcactcatc ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa
    13621 aaggtcattg actaactata ggagatttag aattttatta gcatatacgc ggaagcggtt
    13681 cggcgtgcgg tagatcgagg gttcgtttca cgccatacag accggcaaga cgaacacctt
    13741 tacgttctac tacttcgcca atcagcgccg catcttttcc caatggatgg gaatgtaacg
    13801 ccgccagcac ttgctctgct gcgttgcgtt caacggcgat caccagtttg ccttcgttgg
    13861 caaagttaag ggcgtccagt cccagcagtt cgcaaacgcc gcgcacagca ggtttaaccg
    13921 gcagtgccgc ttctgaaagt tcaataccac aaccgcaggc tgccgcgaac tcatgaacca
    13981 ccgcgtttac accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca
    14041 gcgtctgaat aagcggcgcc agcaccgcgc agtcgctgac cagttctcca tccagcccta
    14101 gttgctcacg caggttaagg atagtcgccc catggtcgcc gagtgtaccg ctaaccagca
    14161 atacatcgcc tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca
    14221 tgccagcggt gttgataaac agtttatctg ccgcgccgcg ctgtaccact ttggtgtcgc
    14281 cagtaacgat ggcaatgcct gccgcacggg cggtttctgc catgctggtc actacggctt
    14341 tcagtgtctc catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa
    14401 ttgcgccact gaccgcaacg tcattggcgg tgccgcaaat cgccagcttg ccgatattac
    14461 cgccaggaaa gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc
    14521 cttccgctac cagctgtgcc aaatcaagac gggcctgatc ttcctgctct gccagccacg
    14581 ggttggcaaa ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc
    14641 tgccgtgggc gagttggata ttattcacgc ttcactctcc tgctgacgat actgatacca
    14701 cgcggcgcac gctccttcgg aggaaaccat cagcgcacca aacgcggttt gaggattaca
    14761 ggtgttacca aacagcgggc attgatgcgg cttacatttg cccgtcaaga cttcgccaca
    14821 acgcgcacgc gggtcatcgc agacctgctg cggtgccggg cggaaatgtg cttcggcatc
    14881 gaatcgttga taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg
    14941 ccattcactg tcgccgttga cacagaacac atcagcaatg gcctgttgcg ccagcaggtt
    15001 accggcatcc ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt
    15061 ctgctccacc agcatcacca cgccttgaag tagatcaagg ggttcgaaac cagcgaccac
    15121 tagcggacga tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact
    15181 gacgtggccc ggcgcgagga acgcgtcgat accgttatct ggctgttcca gcaaactgcg
    15241 cagtgtcggg ataagcgtaa tgtgctggca gaagaagtaa aaattctgca catcgcgggc
    15301 tttcgcctgt tgcagagtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa
    15361 gaataccact ttacgggttg gattctcctg cgccagtttc aacgcatcca ttggcgagta
    15421 aacgatgcgc acatcggcac cgcgtgcttt tgcctgcaac agcgacccct gtttccctgg
    15481 tacgcgcatg gcatcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac
    15541 gcaggtgtcg attctaccca ttggcagtac gcacaccggg caacccggac cgtggataaa
    15601 ctcaacgttt tccggtagca actggtcgag gccgaattta aaaatggcgt gggtatgacc
    15661 gccacacact tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg
    15721 ttcgcgcaga tgctcaatta actgcattac ctgttccggc gcgcgatatt catcaacaaa
    15781 acgcattatt tttcctcgcc atacaacagc gcgccgacat ctggctcaac gtcaaacatg
    15841 ttttgtaagg cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag
    15901 ccaacgtgaa ccagtaccca ctggcccacg cgtggctgac cgttttcatc gcagctgccg
    15961 actaacgtta aatcgacatc gcgttgaatg ccgcagacgt cgactttcgc ctggttgccg
    16021 tcaatggtgc ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca
    16081 gccactggtc catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat
    16141 tgacttcacg ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcagcaagt
    16201 caactttgtt gagcagcatc agcgaggcgg cggcaaacat gtgcggatat ttcagcggtt
    16261 tgtcttcacc ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc
    16321 tggccgggca aacgaggtta ccgacgtttt cgataaacag aataccgtta tcgtccagtg
    16381 gcaggcgcgg cgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt
    16441 tcacctgaat cgctggggtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct
    16501 ggtcaccttc aataaccgcg cacggaacgc tgtctttcag gcgcattaaa gtttccgtca
    16561 gcagggtggt tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg
    16621 cagcaaagcg cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt
    16681 cgacttccag catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac
    16741 cgtaatgcag atcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg
    16801 tgattttcat cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag
    16861 ggttatgttc atcaccctcg atatagaggt tgccttcacc gcaaccgcat gttgtacaca
    16921 tcgctcactc ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct
    16981 gtagcatgtc accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat
    17041 actgttggca tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac
    17101 cttccgccac gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga
    17161 cacaagaaaa tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg
    17221 cggcctgctg ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc
    17281 ctcccattaa ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg
    17341 tgaataatgt cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc
    17401 ttagaaatcg ctttaataac aatgaattaa aagttggcac agaaaatgct taaagctggc
    17461 atctctgtta aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt
    17521 acatcgcaca gcggcatcgt cgcctacagg accagtggca catctactgc aattcgctgg
    17581 ttcaggggat cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca
    17641 aagaactctg tttcgtcctt tttgaacatt ttcgcattta cgtcaccctg gcggatggct
    17701 ttaacagcca caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga
    17761 ttgcgcaggc gcagttgagc attgacggca tgattgatgg caaggtcaac atccgcgatc
    17821 gcgaacaagt tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttatata
    17881 ccgccattga aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg
    17941 tagcatgagc tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg
    18001 tcgatgagtg tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct
    18061 gaggaatgcc tggtgaatcg ttttgtaatt gctgactcca cgctctgtat cggctgccac
    18121 acttgtgagg ctgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc
    18181 ctgcgagtga tgctcaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat
    18241 gcaccctgcg caacggtctg cccggttaac gccatcactc gtgttgatgg tgccgtacag
    18301 ctgaatgaaa gcttgtgcgt aagctgcaaa ctgtgtggca tcgcctgccc gtttggcgca
    18361 attgaatttt ccggcagccg tccgctggat attccggcaa acaccaatac cccgaaagcg
    18421 ccaccggcac cgcctgctcc ggcgcgtgtc agcacattgc ttgactgggt gccaggtatt
    18481 cgcgcgatcg ccgtcaaatg tgacctttgt agctttgatg aacaaggtcc ggcctgcgtg
    18541 cggatgtgcc cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc
    18601 aaacgtaagc gtgagctgac ttttaacacg gactttggcg atctcacctt gtttcagcag
    18661 gctcaaagtg gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcgtggt
    18721 ttgtcgccgc cgctgttctg gcatttctct tttcttttca aaaagcgcta agtggctgga
    18781 tagccggaat tggcggcgcg gtgggtagcc tgtatacggc agccgcgggc ttcactgtgc
    18841 tgactggcgc ggttggcgtg agcggtgcgc tgtcgttggt aagctacgat gtgcaaatct
    18901 ctccgcttaa cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct
    18961 acaacattga ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt
    19021 tgatggctgc cgccgtctgt gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg
    19081 ccgaaatcat ggccctgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt
    19141 ggtttgcgct ggggcgtctt ggcactctgc tgctggcgat tgcctgctgg ctgctgtggc
    19201 agcgttacgg cacgctggat ctgcgcctgc tggatatgcg tatgcaacag ctgccgctcg
    19261 gttccgatat ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc
    19321 tgcacggctg ggtgccacag gcacatgcca acgcctctgc gccagctgcc gcgctgtttt
    19381 ccacggtagt catgaaaatt ggcctgctgg gcattttaac tctgtcacta ctgggcggta
    19441 atgcaccgct gtggtggggg atcgcactgc tggtactcgg catgatcacc gcattcgtcg
    19501 gcggtctgta tgcgttgatg gaacataata tccaacgtct gctggcttac cacaccctgg
    19561 aaaatatcgg catcatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac
    19621 aaccggcgct gattgccctt ggtctggttg gtggtctgta ccatctgctt aaccatagcc
    19681 tgttcaagag cgtactgttc cttggtgcgg ggagcgtctg gttccgtacc ggtcatcgcg
    19741 atatcgaaaa actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag
    19801 tcgggctgat ggcaatggct gcgctgccgc cgctgaacgg ttttgccggg gaatgggtta
    19861 tctatcaatc cttcttcaaa ctgagcaata gtggcgcgtt tgttgcccgt cttctcgggc
    19921 cgctgctcgc cgtggggctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag
    19981 tttatggcgt tactttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtt
    20041 cgccgctcct gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg
    20101 ttgctgcgcc gtggctactg ccgatgctct ctgctgctgt acctctgccg ctggagcctg
    20161 ctaacaccac cgtttctcaa ccaatgatca cgttgttgtt gattgcctgc ccgctgctgc
    20221 cattcatcat tatggcgatt tgcaaaggcg atcgtttgcc gtcgcgttcc cgcggcgcgg
    20281 cctgggtgtg cggctacgat catgaaaaat caatggtgat taccgctcac ggttttgcca
    20341 tgccggtgaa acaggcgttt gcgccggtgc tgaaactacg caaatggctg aatccggtgt
    20401 ctctggtgcc gggctggcag tgcgagggga gtgcgttgct gttccgccgg atggcgctgg
    20461 ttgaactggc ggtgctggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt
    20521 atatccgtta attcaggcgc tggtgctatt tgccgttgcg ccgctgctct ccggtattac
    20581 ccgcgtagca cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg
    20641 cgacattatc aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt
    20701 ccgcctgacg ccgtatgtga tggtgggcgt catgctgact atcgccactg cgctgccggt
    20761 ggtgaccgtc ggttctccgc tgccgcaact gggcgatttg atcaccttac tgtatctctt
    20821 cgccatcgcg cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc
    20881 tatcggcgcg agccgtgaag cgatgcttgg cgtgttggtt gaaccgatgc tgctgcttgg
    20941 tctgtgggtc gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt
    21001 ttatcactgg ccactgagcc agagcatccc gctggtgctg gcgctttgtg cctgtgcatt
    21061 cgccaccttt atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagtt
    21121 gcaggaaggt ccgctctctg agtatagcgg cagcggcttt ggcgtcatga aatggggtat
    21181 cagcctgaaa cagctggtgg tgttgcagat gttcgtcggg gtgtttattc cgtggggaca
    21241 aatggaaacc ttcaccgtcg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact
    21301 ggtggttggc gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga
    21361 tattactccg cgcattacct gggccgggtt tggctttgca tttttagcgt tcgtctcctt
    21421 gctggcggcg tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat
    21481 ctcgccgcgc tgaatgaggc atttcctggc gtcgtgctgg accacgcctg gcagaccaaa
    21541 gatcagctga ccgtgaccgt aaaggtgaac tacctgccgg aagtggtgga gtttctctac
    21601 tacaagcagg gtggctggct gtcggtgctg tttggtaacg acgaacgcaa actgaatggt
    21661 cattacgccg tttattacgt gctgtcgatg gagaagggca ccaagtgttg ggtaacggtt
    21721 cgcgttgaag ttgacgccaa caaaccggag tatccttccg tgacgccgcg cgttccggcg
    21781 gcggtgtggg gcgagcgtga agtgcgcgat atgtacggtt tgattccggt tggtctgccg
    21841 gatgaacgcc gtctggtgct gccggatgac tggccggatg aactttatcc tctgcgtaaa
    21901 gacagcatgg attatcgtca gcgtccggca ccgactaccg atgctgaaac ctacgagttc
    21961 atcaacgaac tgggcgacaa gaaaaacaac gtcgtgccga ttggtccact gcacgtcact
    22021 tctgacgaac cgggtcactt ccgtctgttc gtcgatggtg aaaacattat cgacgccgac
    22081 taccgcctgt tctacgtcca tcgcggcatg gaaaaactgg cggaaacccg tatgggttac
    22141 aacgaagtga ccttcctctc tgaccgtgtg tgcgggatct gcggctttgc tcacagcacc
    22201 gcctatacca cgtcggtgga aaacgcgatg ggtattcagg tgccagaacg cgcgcagatg
    22261 atccgcgcca ttctgctgga ggtagaacgc ctgcactcgc atctgctcaa cctcggcctg
    22321 gcctgtcact ttaccggctt tgactccggc tttatgcagt tcttccgcgt gcgtgaaacc
    22381 tccatgaaaa tggcagagat ccttaccggt gcgcgtaaaa cctacggcct gaacctgatc
    22441 ggcgggattc gtcgcgatct gctgaaagac gacatgattc agacccgcca gctggcgcaa
    22501 cagatgcgtc gtgaagtgca ggagctggtg gatgtgctgc tgagcacacc gaacatggaa
    22561 cagcgcaccg tcggcattgg tcgtctggac ccggaaattg cccgcgattt cagtaacgtt
    22621 ggcccgatgg tccgcgccag cggacacgct cgcgataccc gcgccgatca cccgtttgtt
    22681 ggttatggcc tgctgccaat ggaagtccac agcgagcagg gctgcgacgt tatttcccgt
    22741 ctgaaagtgc gtatcaacga agtctatacc gcgctgaata tgatcgatta cggtctggat
    22801 aacctgccgg gcggcccgct gatggtggaa ggctttacct acattccgca ccgtttcgcg
    22861 ctgggctttg ccgaagcgcc gcgcggcgac gatatccact ggagcatgac cggcgacaac
    22921 cagaagctgt accgctggcg ctgccgtgcc gcgacctacg cgaactggcc gaccctgcgc
    22981 tacatgctgc gtggcaatac cgtttccgat gcgccgctga ttatcggtag cctggaccct
    23041 tgctactcct gtaccgaccg catgaccgtg gtcgatgtac gtaagaagaa gagcaaagtg
    23101 gtgccgtaca aagaactcga gcgctacagc attgagcgta aaaactcgcc gctgaaataa
    23161 ggaatcgcca tgtttacctt tatcaaaaaa gtcatcaaaa ccggcacggc gacttcgtct
    23221 tatccgctgg agccgattgc ggttgataaa aacttccgtg gtaagccaga gcagaacccg
    23281 cagcagtgca tcggctgtgc ggcctgcgtc aatgcctgcc cgtcaaacgc cttaacggtt
    23341 gaaactgacc tcgccacagg agagctggcg tggcagttca atcttggacg ctgcatcttc
    23401 tgtggacgct gcgaagaagt ctgcccgacg gcggcgatta agctgtcgca agaatacgaa
    23461 ctggcggtgt ggaagaaaga agacttcctg caacagtccc gcttcgcgct gtgcaactgc
    23521 cgcgtctgca atcgtccttt cgccgtccag aaagagatcg actacgccat tgcgctgctt
    23581 aagcacaacg gcgacagccg cgcggaaaac caccgcgaaa gctttgagac ttgcccggaa
    23641 tgtaagcgcc agaaatgcct ggtgccgtcc gaccgtattg aactgactcg ccatatgaaa
    23701 gaggccatct gatgagcaat ttattaggcc cccgtgacgc caacggcatt ccggtcccga
    23761 tgacggtgga tgaatccatc gccagcatga aggcgtcgtt actgaaaaaa atcaaacgtt
    23821 ctgcctacgt ttatcgcgtg gactgcggcg gctgtaacgg ttgcgaaatc gaaattttcg
    23881 ccacgctttc gccgctgttc gatgcagaac gcttcggcat taaagtcgtt ccgtcaccgc
    23941 gtcatgcgga tattttactg tttaccggcg cggtcacccg tgcaatgcga tcccctgcgc
    24001 tgcgtgcgtg gcagtccgcg ccggacccga aaatctgtat ctcttacggt gcctgcggta
    24061 acagcggcgg gatcttccac gatctctact gcgtgtgggg cggtacggat aaaatcgttc
    24121 cggtagatgt ctacatccct ggctgcccgc caacgcctgc cgccacgctg tacggctttg
    24181 cgatggcgct cggcctgctg gagcagaaaa tccacgcccg tgggccgggt gagcaagatg
    24241 agcagccggc agacattcta catcctgata tggtgcaacc gttgcgtgtg aaagtggacc
    24301 gtgaagcgcg tcgtctggcg ggttatcgtt acggtcgcca gattgccgat gatttcctga
    24361 cacagttagg gcagggcgaa gaacaggttg cacgctggct ggaagcggaa aacgatccgc
    24421 gtctgaacga gattgtcagc catctgaatc atgttgttga agaggcgcgt atccgatgag
    24481 tgaaaaggtg gtgttcagtc aactgagccg taaatttatt gatgagaacg atgccacgcc
    24541 cgccgaggcg cagcaggtgg tctattacag cctggcgatt ggtcaccacc ttggggttat
    24601 cgattgcctg gaagcggcac ttacctgccc gtgggatgac tatctggcat ggattgccac
    24661 tctggaggcg ggcagcgaag cccgccgtaa aatggaaggc gtgccgaaat atggtgagat
    24721 cgtcatcgac attaaccatg tgccgatgct ggccaacgca ttcgataaag cccgggcagc
    24781 gcaaacttcg cagcagaagg aatggagtac aatgctgtta agtatgctgc atgatattca
    24841 tcaggaaaac gccatctatt tgatggtgag gagactgcgt gactgacgtt ttactttgtg
    24901 ttggcaatag catgatgggc gatgatggcg caggcccgct actggcggaa aagtgcgccg
    24961 ccgcgccgaa aggtaactgg gtagtgattg acggcggtag cgcaccggaa aacgacattg
    25021 tcgctatccg cgaactgcgc ccgacacggc tgctgattgt cgacgccacg gatatggggc
    25081 taaaccctgg cgagatccgc atcatcgacc cggctgacat cgccgagatg tttatgatga
    25141 ctacccataa catgccgctg aactatctta tcgaccagtt gaaagaagat attggcgaag
    25201 tgattttcct tggcatccag ccggatatcg tcggctttta ctacccgatg actcagccga
    25261 ttaaagatgc ggtggaaacg gtgtatcagc ggctggaagg ctgggaagga aatggcggct
    25321 tcgcgcagtt agcggtggaa gaataattct tcattaggga attcagggaa ttgaacatca
    25381 aaacagggat gtgcgctttt ttattgagct tagtgctgcc tgcacaggcc accagcttta
    25441 ccgaatatct gccaatgagt gacagcgaat acgcgcaaaa gcgggcgctg aaacctctcc
    25501 tgaccatgcc ttatgatgcc gagcaaacct ggcattttag aaaagtgggc gtctgacacg
    25561 ttactacaga gagaacaaag aatgactgct aatgctgcac gcgctgttaa agcaacaaga
    25621 gaactggtca atgcagtccc ctttctggga ggcagtgact cagaagatga ttaccgtgaa
    25681 gcactggaac tggtcgaata tttgattgaa gaagatgata ctaatccact tatcgacttt
    25741 ctggcgagcc gcattgcaga atatgaaaac aataacgaaa agtttgcaga gttcgacaaa
    25801 gccgttgcag caatgcctgt tggtgtggcg ttacttcgca cgctgattga tcaacataat
    25861 ctgacctatg cagatttgaa gaacgaaatc ggctctaaat ccctggtcag ccagatttta
    25921 tctggacaac gttccctgac gatatcccac attaaggcac tttcagcccg atttggcgtt
    25981 aagccggaat ggtttctttg actgatggca gacgctaaat cgcctgatgc gctacgccta
    26041 tcgggctttg aaagggcgca gattcttaat tttgcgcgcc cttgtttgtc attcaggctg
    26101 ttgtctttac tactcttaat ttatcagtaa gctcaatcag atatgctttt gctttttcgc
    26161 gtgctcgccc ctcgaaaagc attatccact catgtgtgtc gtcgattgtt tgtaaacggc
    26221 cctggagttt tttgctgaaa gtcgacgtga gctgataacc gtcgagttga tcaatttgtt
    26281 tactcgatgt gatttttttc ccggctgctt tttgaatgat aagttcaacg agaccgataa
    26341 cattattatc gtaattatcg tagagaaatt cttcatctcc ctgaaatccg aacaaatctt
    26401 catgctggct taagcgttga acaattgact cggcatcaaa ggtcgaatca tcttgcagtt
    26461 gttcctctac ccaattaatc aaatccagcc cttcgtcgct ttcgagagcc tccattcccc
    26521 atgaacccat tttgtatcct tacctgttat ttcagtcgaa atagtattat tcaataaagg
    26581 ttcatcgcta atctgcgatg aacctcaaaa tacaatcata tggatagctg acaaagtggt
    26641 cgttgttcac gccgtatccg gcacggttgc ttcccttaaa tcctccccgt tactggcaat
    26701 tactttttta taccaccaga acgacttgtc ggaaaaaagt acccagatgt ggactggcaa
    26761 cgataaaata ttcattagaa aacggattct ctctgcctta ctacgataaa tactgtcatc
    26821 a
//
LOCUS       DACADA010000057        26088 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.058, whole genome shotgun sequence.
ACCESSION   DACADA010000057 DACADA010000000
VERSION     DACADA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.058"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            47..388
                     /locus_tag="ISB17_002296"
     CDS             47..388
                     /locus_tag="ISB17_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980226.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            434..871
                     /locus_tag="ISB17_002297"
     CDS             434..871
                     /locus_tag="ISB17_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAP1980227.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(913..1908)
                     /locus_tag="ISB17_002298"
     CDS             complement(913..1908)
                     /locus_tag="ISB17_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP1980228.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2083..2382
                     /locus_tag="ISB17_002299"
     CDS             2083..2382
                     /locus_tag="ISB17_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980229.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2477..3388
                     /gene="glyQ"
                     /locus_tag="ISB17_002300"
     CDS             2477..3388
                     /gene="glyQ"
                     /locus_tag="ISB17_002300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP1980230.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3398..5467
                     /gene="glyS"
                     /locus_tag="ISB17_002301"
     CDS             3398..5467
                     /gene="glyS"
                     /locus_tag="ISB17_002301"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP1980231.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5789..5941
                     /gene="hokA"
                     /locus_tag="ISB17_002302"
     CDS             5789..5941
                     /gene="hokA"
                     /locus_tag="ISB17_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAP1980232.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFL
                     ACKLKE"
     gene            complement(6129..6341)
                     /gene="cspA"
                     /locus_tag="ISB17_002303"
     CDS             complement(6129..6341)
                     /gene="cspA"
                     /locus_tag="ISB17_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAP1980233.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6622..6912)
                     /locus_tag="ISB17_002304"
     CDS             complement(6622..6912)
                     /locus_tag="ISB17_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAP1980234.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7346..8056
                     /locus_tag="ISB17_002305"
     CDS             7346..8056
                     /locus_tag="ISB17_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAP1980235.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8106..9080)
                     /gene="ghrB"
                     /locus_tag="ISB17_002306"
     CDS             complement(8106..9080)
                     /gene="ghrB"
                     /locus_tag="ISB17_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAP1980236.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVVD"
     gene            complement(9184..9843)
                     /locus_tag="ISB17_002307"
     CDS             complement(9184..9843)
                     /locus_tag="ISB17_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAP1980237.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            9996..12329
                     /locus_tag="ISB17_002308"
     CDS             9996..12329
                     /locus_tag="ISB17_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP1980238.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(12298..12738)
                     /locus_tag="ISB17_002309"
     CDS             complement(12298..12738)
                     /locus_tag="ISB17_002309"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1980239.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12735..13298)
                     /gene="tag"
                     /locus_tag="ISB17_002310"
     CDS             complement(12735..13298)
                     /gene="tag"
                     /locus_tag="ISB17_002310"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP1980240.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            13456..14154
                     /locus_tag="ISB17_002311"
     CDS             13456..14154
                     /locus_tag="ISB17_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1980241.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            14383..15591
                     /locus_tag="ISB17_002312"
     CDS             14383..15591
                     /locus_tag="ISB17_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980242.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            15915..17606
                     /gene="eptB"
                     /locus_tag="ISB17_002313"
     CDS             15915..17606
                     /gene="eptB"
                     /locus_tag="ISB17_002313"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAP1980243.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            17698..17774
                     /locus_tag="ISB17_002314"
     tRNA            17698..17774
                     /locus_tag="ISB17_002314"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17732..17734,aa:Pro,seq:cgg)
     gene            18518..20125
                     /gene="dppA"
                     /locus_tag="ISB17_002315"
     CDS             18518..20125
                     /gene="dppA"
                     /locus_tag="ISB17_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAP1980244.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            20432..21451
                     /gene="dppB"
                     /locus_tag="ISB17_002316"
     CDS             20432..21451
                     /gene="dppB"
                     /locus_tag="ISB17_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAP1980245.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            21461..22363
                     /gene="dppC"
                     /locus_tag="ISB17_002317"
     CDS             21461..22363
                     /gene="dppC"
                     /locus_tag="ISB17_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAP1980246.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            22374..23357
                     /gene="dppD"
                     /locus_tag="ISB17_002318"
     CDS             22374..23357
                     /gene="dppD"
                     /locus_tag="ISB17_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAP1980247.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            23354..24358
                     /gene="dppF"
                     /locus_tag="ISB17_002319"
     CDS             23354..24358
                     /gene="dppF"
                     /locus_tag="ISB17_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAP1980248.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(24388..25659)
                     /locus_tag="ISB17_002320"
     CDS             complement(24388..25659)
                     /locus_tag="ISB17_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1980249.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
BASE COUNT         6349 a         6388 c         6810 g         6541 t
ORIGIN      
        1 ttcgttggtt actcgtaatg tatcgctggt agatatggag atcgttatga aaacctcaaa
       61 gactgtggca aaactattat ttgttgtcgg ggcgctggtt tatctggttg ggctatggat
      121 ctcatgccca ttgttaagtg gaaaaggcta ttttcttggc gtgttaatga cggcaacttt
      181 tggcaactat gcatatcttc gcgcagaaaa acttgggcaa ctggatgatt tttttatcca
      241 cgtctgtcag ttggtagcgt taatcactat cggtctcttg tttatcggtg ttttaaacgc
      301 acctatcaat gcttatgaaa tggtgatcta tcccatcgcc ttttttgtct gcttgtttgg
      361 tcaaatgcgt ttgtttcgct cggcatgagc aacataaagt tcttacacat tcaggaatga
      421 aaggaatact gtgatggaca acaaaatatc aacctattct ccggcattta gtattgtttc
      481 atgggtagcc cttattggcg gtattgttac ctatctgtta ggactatgga atgcagagat
      541 gcagttaaat gaaaaagggt attattttgc cgtactggta ttaggtttgt tttccgcggc
      601 gtcttatcaa aagaccgttc gggacaagta tgaaggcata cctaccaccc ccatttatta
      661 tatgacttgc ctgacggtct ttattatctc tgttgcgttg ttgctggtag gtctatggaa
      721 tgcaacgtta ttactcagcg aaaaaggctt ttatgggctg gctttcttct taagcttgtt
      781 tggtgcagta gcggtgcaga agaatattcg tgatgccgga ataaacccac caaaagaaac
      841 acaggttacc caggaagaat acagcgaata attcacgtaa gcccggtcag tccaatgtga
      901 ccgggcttct acttaactca ctaatctgtt tctgtcgatt cgttgtacca gcatagaaag
      961 taataaactc actgccagcg tcgcacaaaa gatccaaata atatccagta ttggccagtt
     1021 tttaagctca atcccccggg tgcgcagtgc atggataatc aatgcatgga acccgtatat
     1081 acctaacgaa tggcgggaga ttaagccaag tccgcaaatg gtacgcgtat acagcgtgtt
     1141 tttaaccaga gtcaataacg cgattgcgca gataaaaacc atcggcccac agtaaagata
     1201 ccaggtatcg gcaaaatttc cgcgccactg taattcatat aatgtcccac gagagataat
     1261 aaaaaccccc gtcgcaaaca gtgcggcgct cacccacgac agtgctttat gctgtgtgtc
     1321 catcatccct atagcgcggc ccaacatgcc ataaaggatg tagtaaaaag tatcgccatt
     1381 gatatataag ttaattggca gccattcaaa accgtcgatt ttctgcggca cggtgtttgg
     1441 gttagcgatg ataccaatca ccaccattag caccagcaac atttttccgc cgacgttctt
     1501 cacctgaatc agcggtgaaa ccagataaat cactgcaatc gcgaagaaaa accataaatg
     1561 gtaaaacact ggcttttgca gcaggttttt cagcgctaac tccacattga tggaggtaaa
     1621 cagcgcaatg tagagcagcg cgattgcgct ataaaaaaac aggcataagc cgatacgcaa
     1681 gaaatgacgc gactgggcgc tgcgttcgcc aaaaaagaga tagccggaaa tcatgaaaaa
     1741 tagcggcacg ctgacacgag aggcagaatt cagaacattg gcgatatccc aggtgatggg
     1801 gctaacacta tgagcattga tcacatacca ggtagtggtg tgaatcatca ccaccattaa
     1861 acacgctatc cctcgcaggt tatcaatcca gtaaattttg ggctgcatct gtgtctctgt
     1921 atctggttaa aaaaagtctg accgataaat cattcagttg gcgcactgga ataatctgag
     1981 ttttatcact acagcttata gaggcttaag gaaattcgta agatatcagc cactataccg
     2041 atataaataa taagactcac ctgcaaacca gacggtaatt taatgatgat gaacgctttc
     2101 tttccggcaa tggcgcttat tgtgctagtg ggttgttcta caccgccacc cgtgcagaaa
     2161 gcacaacggg taaaggttga tcctctgcgt tcgttgaata tggaagcgtt atgcaaggat
     2221 caggcggcaa aacgttataa caccggcgag caaaaaatcg acgtcaccgc cttcgaacag
     2281 ttccagggaa gctatgaaat gcgcggttat accttccgga aagagcagtt tgtctgttct
     2341 tttgacgcgg atggccattt tttgcatctt tccatgcgtt aagccctgct ttttcccgtt
     2401 tcgtactgta tatcttccat ccagcgggta tactgatccc ttcctttaaa tccacacgta
     2461 tccagcacga aataatatgc aaaagtttga taccaggacc ttccagggct tgatcctgac
     2521 cttacaggat tactgggctc gccagggctg caccattgtt caaccattgg acatggaagt
     2581 cggcgcggga acctctcacc caatgacctg tctgcgcgcg ctggggccag aaccgatggc
     2641 ggctgcttat gttcagcctt ctcgtcgccc gaccgatggt cgctacggcg aaaaccccaa
     2701 ccgtttacag cactactatc agttccaggt ggtcattaag ccatcgccgg acaatattca
     2761 ggagctgtac ctcggttctc tgaaagagct gggcatggac ccgaccatcc acgacatccg
     2821 tttcgtggaa gataactggg aaaacccgac gctgggtgcc tgggggctgg gctgggaagt
     2881 gtggctaaac ggcatggaag tgacgcagtt cacttacttc cagcaggttg gtggtctgga
     2941 atgtaaaccg gttaccggtg agatcaccta cggtctggaa cgtctggcca tgtacattca
     3001 gggcgtagac agcgtttacg acctggtctg gagcgacggc ccgctgggta aaaccaccta
     3061 cggcgacgtg ttccatcaga acgaagtgga gcagtccact tacaacttcg aatacgcgga
     3121 tgtggacttc ctgttcacct gcttcgagca gtacgagaaa gaagcgcagc aactgctggc
     3181 gctggaaaat ccgctgccgc tgccagccta cgagcgtatt ctgaaagccg cccacagctt
     3241 caacctgctg gatgcgcgta aagccatctc cgtcaccgag cgtcagcgct atattctgcg
     3301 cattcgcacc ctgaccaaag cagtggcaga agcatactac gcttcccgtg aagccctcgg
     3361 cttcccgatg tgcaacaaag ataagtaaga ggcggctatg tctgagaaaa cttttctggt
     3421 ggaaatcggc actgaagagc tgccaccaaa agcactacgc agcctggctg agtcctttgc
     3481 tgcgaacttt actgcggagc tggataacgc tggcctcgca cacggcaccg ttcaatggtt
     3541 tgctgctccg cgtcgtctgg cgctgaaagt cgctaacctg gcggaggcgc aaccggatcg
     3601 tgaaatcgaa aaacgtggtc cggcaattgc tcaggcgttc gacgctgaag gcaaaccgag
     3661 taaagcggca gaaggttggg cgcgtggttg cggtattacc gttgaccagg ctgagcgtct
     3721 gactaccgat aaaggcgaat ggctgctgta tcgcgcccat gtgaagggcg aaagcaccga
     3781 agcactgctg ccgaatatgg ttgcgacttc gctggcgaaa ctgccgatcc cgaaactgat
     3841 gcgttggggc gcaagcgacg tgcactttgt gcgtccggtt cacaccgtga ccctgctgct
     3901 gggcgacaaa gttattccgg ctaccattct gggtattcag tccgatcgcg tgattcgcgg
     3961 ccaccgcttt atgggcgagc cggaattcac tatcgacaat gccgatcagt atccagaaat
     4021 tctgcgtgag cgcgggaaag tcatcgccga ttacgaagaa cgtaaggcga agattaaagc
     4081 cgatgctgaa gaagcagcac gtaagattgg cggtaacgct gatttaagcg aaagcctgct
     4141 ggaagaagtg gcttcgctgg tggagtggcc ggtcgttctg accgcgaaat tcgaagagaa
     4201 attcctcgcg gtgccgtctg aagcactggt ttacaccatg aaaggtgacc agaagtactt
     4261 cccggtgtat gcgaacgacg gcaaactgct gccgaacttt atcttcgttg ccaacatcga
     4321 atcgaaagat ccgcagcaga ttatctccgg taacgagaaa gtcgttcgtc cacgtttggc
     4381 ggatgccgag ttcttcttca ataccgaccg taaaaaacgt cttgaagata acctgccgcg
     4441 cctgcaaacc gtgttgttcc agcaacagtt ggggacgctg cgcgacaaaa ctgaccgcat
     4501 tcaggcgctg gccggctgga ttgctgaaca gattggcgct gacgttaacc acgctacccg
     4561 tgcgggcctg ctgtctaagt gtgacctgat gaccaacatg gtcttcgagt tcaccgacac
     4621 ccagggcgtt atggggatgc actacgcgcg tcacgatggc gaagcggaag acgtagctgt
     4681 cgcgctgaat gagcagtatc agccgcgctt tgccggtgat gacctgccgt ctaacccggt
     4741 agcctgtgcg ctggcgattg ctgacaagat ggataccctg gcgggtatct tcggtatcgg
     4801 ccagcatccg aaaggcgaca aagacccgtt tgcgctgcgt cgtgccgcac ttggcgtgct
     4861 gcgtattatc gttgagaaga acctcaacct tgatctgcaa acgctgaccg aagaagcagt
     4921 gcgtctgtat ggcgataagc tgactaatgc caacgtggtt gatgatgtta tcgactttat
     4981 gctcggtcgc ttccgcgcct ggtatcagga cgaaggttac accgttgaca ccatccaggc
     5041 ggtactggcg cgtcgtccga ctcgtccggc tgatttcgat gcccgtatga aagcggtatc
     5101 gcacttccgt accctggaag cagctgcagc actggcggcg gcgaacaagc gtgtatctaa
     5161 catcctggcg aaatctgatg aagtgctgag cgatcgcgtg aatgcctcta ctctgaaaga
     5221 gccggaagaa attaaactgg cgatgcaggt tgtggtgcta cgtgacaagc tggagccgta
     5281 ctttgcggaa ggtcgttacc aggatgcgct ggtcgaactg gctgagctgc gtgaaccggt
     5341 cgatgctttc ttcgataaag tgatggtcat ggttgatgat aaagaattgc gtatcaaccg
     5401 tctgaccatg ctggaaaaac tgcgcgaact gttcctgcgc gttgcggata tttcgctgtt
     5461 gcagtaataa cgccgttatt aaaaagcctg ccatctggca ggcttttttt atcgctaaat
     5521 aatacagcaa cctttaataa tcttctgctg aataaagatt atctcatata ttaattttat
     5581 gagatttttt taggattata tcaaggagaa gaaacgaact tattaagctg gaatagccac
     5641 gggtgcttga ggctgtttgt ctcaggtatt caccgaaagg cagacagaga aaagccccac
     5701 ctgactataa atcaaagtga ggctacccta tgcctgaaca ccataaggtt agcctcttac
     5761 tcgttggaaa tcaacacagg aggctgggat gccgcagaaa tatagattac tttctttaat
     5821 agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac tgtgtgaatt
     5881 gcatattaaa caggagaatt atgagctggc cgcgttttta gcctgcaaat tgaaagagta
     5941 agaatcttcg gcgggaagtt attcccgcct ttcttacggc gttacgcatt ctcattacac
     6001 ccaaatttat tcttcacaaa aataataata gattttatta cgtgaccgat tatttatttc
     6061 ctgaaaaaca aataaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta
     6121 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
     6181 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
     6241 aagtgtacga acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
     6301 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
     6361 ttaattgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
     6421 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
     6481 tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggtgggt gatgcaacaa
     6541 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgttatcag gaccgtaaac
     6601 agaaataaag tggataaaag cctattccat caactgctta cttaatgccg ggttggcttg
     6661 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
     6721 ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt
     6781 cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
     6841 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
     6901 tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct
     6961 cattaagaat agtcgggaaa aacagacaca gcggggtgta gtcagggcat actctgacta
     7021 tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
     7081 cgatatatct cttaccactc ccggtttgta aagatttcga aaatctaacg ttctgcgaag
     7141 gcgtaaatct ctggctacaa aaaaggcaac ggcaatatca ttttttaaca atgaaacaac
     7201 aaattaacct tgtttcattt tctccatgat tttaccagga agattatgta gaaagggtat
     7261 cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct
     7321 ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc
     7381 gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga
     7441 tcagcgtaaa gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct
     7501 accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat
     7561 tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggttgt
     7621 agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg
     7681 tgaaatgaac ggttcgctgg gtgtgctggc gcaacagctg caaaatgcga agttgcaggc
     7741 cgatgctgcg cactctgcgt taaaacagag tgatgacctg aaaccggtct ttgatcaggc
     7801 attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca
     7861 aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt
     7921 gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa
     7981 actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt
     8041 tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact cctcgcagtc
     8101 gcggcttagt ccacgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca
     8161 ttaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat
     8221 ccaatatgcg gtaatgcgac gacgttggcc attgagagca acggcgaatc tacggacagt
     8281 ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cccctttctg caatgcagca
     8341 atcagtgcat tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat
     8401 ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatccgt taacggcagg
     8461 atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg
     8521 aagcgttctt ctgcttcttt atggtggcga cgcgcgttat agaggatggg catgttgaag
     8581 ccaaagtgcg cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt
     8641 gttttatggt gaacgtcagt gccgtaccag tccgggccta tactcgcggt ccattcgcct
     8701 gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc
     8761 agcgtatcgg cgacggtttc tgttaatacg gttggtgtgt gcatcagcag aatttttcgg
     8821 gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc
     8881 agtttcggca ttttttccag caatgcggca tcaacattct catttgaacc cagcaagcct
     8941 tcagcttcgg caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgctacc
     9001 tggtgaacgg tgaaatgttc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag
     9061 aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg
     9121 aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct gacatgaaag
     9181 ggattacagt gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt
     9241 gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc
     9301 cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat
     9361 gttcaggtcg tgactccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg
     9421 atactctttc agtaccattg ccacgccggt cagggtgtta gcgcccgccg gtttcagggt
     9481 tgcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct
     9541 gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc
     9601 catgtaataa ccaacgccgc cgcccagagc tgcgcctgct gctgcgccaa tgagcgcgcc
     9661 tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccaa cgagagagcc
     9721 cagacctgcg ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt
     9781 acagccagat accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt
     9841 cattgttaat ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat
     9901 gccgcgtgag catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac
     9961 tgctcaatac atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt
    10021 ccgttctgac tgccgcccac tgggggccta tgctggttga aaccgacggc gaaaccgtgt
    10081 ttagctcgcg tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg
    10141 accaggttca cagcaatacg cgggtgcgat ttccaatggt gcgcaaaggc tttcttgcgt
    10201 caccggaaaa tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg
    10261 aggcgctgga gcttattcac catcaacata aacgcattcg tgaggcttat ggtccggcat
    10321 cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat
    10381 tattacaacg ctatatggcg ttggcaggcg gttatactgg gcatctgggg gattattcga
    10441 ccggcgcggc acaggcgatc atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc
    10501 aaaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgctgtgg agtgctaacc
    10561 cactcaatac gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt
    10621 ccgcactgcg tgacagcggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg
    10681 tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgt
    10741 tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg
    10801 cgcgttgcac cacaggttat gctgtcttcg cctcttattt gctgggcgag agtgacggaa
    10861 tagcgaaaaa cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg
    10921 agctggcggc tattttccac caaaatacaa ccatgctgat ggcaggctgg ggaatgcagc
    10981 gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc
    11041 aaatcggcac acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc
    11101 ccacgcgccg cgctgcggtg ctctcttcca tgcagggcag cttgccgggt ggcaccgatg
    11161 cggtagataa aatccctgtc gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat
    11221 atcaacataa cggtatggac cgacatttcc cggatattcg ttttatctgg tgggcgggtg
    11281 gtgccaactt tactcatcat caggatacca atcgcctgat ccgtgcctgg caaaaaccgg
    11341 agctggtggt gatctccgaa tgcttctgga cggctgcggc gaaacacgcg gatatcgttc
    11401 tgcctgcgac cacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc
    11461 agcatctggt gccgatgaag caagtggtgc cgccacgcga tgaagcgcgt aatgattttg
    11521 atgtttttgc cgagttgagt gaacgttggg agaagggcgg ttatgcacgt tttacggaag
    11581 gaaaaagtga gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa
    11641 gccagcaggt tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga
    11701 tgccggaaaa cccggacagt gagcggttta ttcgcttcgc cgattttcgc cgcgatccgc
    11761 aggcgcatcc attaaaaacc gccagcggta agattgaaat cttctcgcag cgtattgccg
    11821 attacgctta tccggattgc ccggggcatc caatgtggct ggcgccggac gaatggcagg
    11881 gtaatgccga accggaacag ttgcaggtac tttctgctca tccggcgcat cgtctgcaca
    11941 gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt ggcaaatcgt gagcctgtca
    12001 ccattcatcc tgacgatgcc cagacgcgcg gcatacaaga tggcgatact gttcggctgt
    12061 ggaactcacg cgggcaaatt cttgccggcg cggtcattag cgagggaatt aaacctggcg
    12121 tgatttgtat tcacgaaggc gcatggccgg atttggattt aaccgctgac ggcatttgta
    12181 aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct
    12241 gtgcgggtaa tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacagtta
    12301 cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca
    12361 tgcgcaatcg acaatgtgaa aaccctgcgc atggtagaaa tctatcgccg gttgattttt
    12421 ttgataaacc tccagcatca gatggggatg gcgctgctgc acatactgca tcagcgcctt
    12481 accaataccg cgcctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc
    12541 cataatgctg acaaaaccga gaagcttacc gtcttcttcc cagacccagt tttgcgcgtt
    12601 ggcaagatac gcatcccgca ccagcggaat gcagtcacgc cagtaactcg attttataaa
    12661 gggatgcccc caggttgtac tttccagcca cagttcgagg agcgcgggga gttctgaacg
    12721 ttgcgcttcc cgaatcatgg tttatttccc ggatggcaac agcagccaac cacatgatca
    12781 ttcaccagcc cacatgcctg cataaaggag taacagattg tggtaccgac aaacttaaaa
    12841 ccacgttttt ttagtgcctt agatagggcg tcggaggcgg gtgtagatgt ggggatttcg
    12901 ctcaacgttg tggcttgtgt cacctgtggt tgatgattga caaacgacca gacaaagtcg
    12961 gcaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc
    13021 tgaattttcc cacgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct
    13081 tcctgcattg cggcaacttt caccggatcg aactgatgaa agcatgcgcg atagttttcg
    13141 cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttccag gcagatcatt
    13201 tcgaacagtt ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata
    13261 taaagcgggt cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg
    13321 atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca
    13381 gggctactat gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc
    13441 cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctt
    13501 gcgagcgtgg taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat
    13561 atcgttgata cgcaacccgg acattccaca gagcgttaca cctgggatag cgatcatcaa
    13621 cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg
    13681 gaagtcaatc tggcggaaga aacccctgtg gatgtgacca gcagtatgag tatgggctgg
    13741 aattttcctt tatatgagca ggttacaacc ggcccggtcg cggcattaca ttacgatggc
    13801 acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctggc cgatccgtta
    13861 tggcatgcca gcgtgagtac cttaggctgg cgtgttgact cccggcttgg cgatctccga
    13921 ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca
    13981 ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc
    14041 gctgatatgt tgctcaatca aaatattgct gcctatgctg cgctatctca ggcagaaaat
    14101 accactaaca atagcgacta tctgtatacg atgggggtta gtgccagatt ttaacgttat
    14161 agatacaatt gaaacccata aataacaact aatgtggcga tagtggggac taacttacta
    14221 aataataaat ttggcgaata attgtcgagt cattcattcc tgaactaatg catttcattc
    14281 cgttctgatg gcatttcatg ccgtttttcc ccaggcataa agcgcacttc gttatggttg
    14341 tcagcagaga tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat
    14401 cagcgtactc gctggctgac actcatcggt actatcatta cccagtttgc tctggggtcg
    14461 gtttatacct ggagtctgtt taacggtgcg ctttctgcca agctgggggc tcctgtaagc
    14521 caggttgctt tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg
    14581 ggcaaattgc aggaacgttt tggtgttaaa cgcgtcacta tggcttccgg cattttgctg
    14641 ggattaggtt tcttcctgac agcacattcc aacaacctga tgatgctgtg gttaagcgcc
    14701 ggtgtgttgg ttggtctggc ggatggtgcg ggttacctgt tgacgctctc taactgcgtg
    14761 aagtggtttc cggagcgtaa gggacttatc tctgcttttg ctatcggttc ttatggtctg
    14821 ggcagcctgg gttttaaatt tatcgacacg cacctgctcg aaacggtggg tctggaaaaa
    14881 acctttgtta tttggggcgc gattgtactg gtgatgattg tctttggcgc aacgttaatg
    14941 aaagatgcgc cgaagcagga agtgaaaacc agcaatggtg tggtggagaa ggactacacc
    15001 ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggttatgtt cctgactgcg
    15061 tgcatgagtg gtctgtatgt gattggtgta gcgaaagata tcgctcaaag tctggcgcat
    15121 cttgatgcaa tttccgcagc caatgctgtg acggttattt ccatcgccaa cctttctggt
    15181 cgtctggtgc tgggcattct gtctgacaaa atcgcccgta tccgtgttat caccattggt
    15241 caggtgattg cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg
    15301 ttctttgcag cgattgcctg tgtggcattt aactttggtg gcacgattac ggtgttcccg
    15361 tcactggtca gtgagttctt cggcctcaat aacctggcga aaaactacgg cgtcatttac
    15421 ctcggtttcg gtatcggcag catttgtggt tcgattatcg cttcactgtt tggcggattc
    15481 tatgtgactt tctacgtgat tttcgccctg ctgattctgt cactggcgct ttctacgacc
    15541 attcgccagc cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc
    15601 gtgagctact gggcttcagc ttcagtgtgc atcaaacgca tcaacgaaat gtataaacaa
    15661 taacgcgtaa aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag
    15721 tctcttttca aacttgcatt tttgtaaatt tgtgcttcgt gcacactctt tccccacact
    15781 ttttcccttt gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc
    15841 cgcacttttc ccctgcctgg cctgaaaagc cactaagcag ggtgttatca cctgtttgtc
    15901 cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc
    15961 ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttaccgccgc tttggcagct
    16021 atgcgcacga ttttaccgtc tggaaaggca tttctgctgt tgttgaactg gccgccaccg
    16081 tactggtgac cttcttttta ctgcgtctgc tttcgctgtt tggccgccgc agctggcgta
    16141 ttctggcatc gctggtggtg ctcttttctg caggtgccag ctattacatg accttcctta
    16201 atgtagtcat tggttatggc atcatcgcat ccgtcatgac caccgatatc gacctgtcaa
    16261 aagaagttgt tggtctgaac tttattctct ggttaatcgc cgtaagcgcg ttgcctctta
    16321 tccttatctg gaataaccgc tgtcgttaca ccttgctccg acaactgcga accccagggc
    16381 aacgtattcg cagcctggcg gtcgtcgtac tggcaggtat tatggtttgg gcaccgattc
    16441 gtttgctgga tatccagcag aagaaagtgg agagggcgac aggtgttgat ttgccgagtt
    16501 atggcggcgt cgtcgcgaac tcttatctgc catcaaactg gctttcggca ttaggtcttt
    16561 atgcctgggc gcgggtcgat gaatcttccg ataacaattc tctccttaac cctgcgaaga
    16621 agtttacgta ccaggctcca caaaacgttg atgatacata tgtcgttttc attattggtg
    16681 aaaccacgcg ctgggatcat atgggcattt tcggctatga acgcaatacc acaccgaaac
    16741 tggctcagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatact gcaaccaaac
    16801 tctcactgcg ttgcatgttt gtacgtcagg gtggagcaga agataatccg cagcgcacat
    16861 taaaagaaca gaacattttc gcggtattga agcagttagg attcagttct gatctctacg
    16921 ctatgcaaag cgaaatgtgg ttctacagca atacgatggc ggacaacatt gcttatcgtg
    16981 agcagattgg tgcggagcca cgtaaccgtg gcaaaccggt agatgatatg ttgctggtag
    17041 acgaaatgca gcaatcgctg gggcgcaacc cggatggtaa gcatctgatc attctgcata
    17101 ccaaaggctc acactttaac tacacccagc gttacccgcg cagctttgcg cagtggaagc
    17161 cggaatgtat tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcatacgaca
    17221 actcggtgac ctatgtggat cactttattt ccagcgtgat tgatcaggtt cgcgataaga
    17281 aagcgattgt gttctatgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc
    17341 acggcacgcc gcgtgaactg gcaccgccgg aacagttccg tgtgccgatg atggtctgga
    17401 tgtcggataa atatctggaa aatccggtca atgcgcaggc atttgcgcag ctgaaaaaag
    17461 aagccgacat gaaagtgcca cgccgtcatg tagagctgta cgacaccatc atgggttgtc
    17521 ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgc cacatcccgc
    17581 aggcaagaga ggcggcggct aactaagagg cctgctgact ttctcgccga tcaaaaggca
    17641 ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg
    17701 tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc
    17761 tctatcaccg accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgtaac
    17821 tcatgaggaa gagaaccccc gggggcgggg aggttcgact cgacgtaggc ctgataagac
    17881 gcaccagcgt cgcatcaggc aaatgtaccg gcccattgtc ggatgcggcg cgaccgcctt
    17941 atccgatcta cacaccacac atttccctgt caaatttgcg ccccatatct cactatccat
    18001 taacggattt gtgacagaaa ccattgtctt tttttatatt tgcttaaatc tttttttgcg
    18061 ttacttatgg ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg
    18121 ctcagatatc cacctttacc tcagattttt ttcattaaat gcataaaact taatcaccaa
    18181 atgttgctaa atattaagct gattgttctg gttgtgttgg cgtagcacaa atttctctgc
    18241 tgcaaatagc tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa
    18301 atcccggttg gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg
    18361 cattttatgg aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca
    18421 taacgttgac ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa
    18481 gggttaaaac aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc
    18541 agggatgctg aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc
    18601 taaaactctg gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac
    18661 ctccggcacc acctatgacg cctcttccgt accgctttat aaccgtctgg ttgaatttaa
    18721 aatcggcacc actgaagtca tcccgggcct cgctgaaaag tgggaagtca gtgaagacgg
    18781 taaaacctat accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa
    18841 accgacgcgc gaactgaatg ccgatgatgt agtgttctcg ttcgatcgtc agaaaaacgc
    18901 gcaaaacccg taccataaag tctctggcgg cagctacgaa tacttcgaag gcatgggctt
    18961 gccagagctg atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac
    19021 tcgcccggaa gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa
    19081 agaatatgct gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat
    19141 cggtaccggc ccgttccagt tacagcagta ccagaaagat tcccgtattc gctataaagc
    19201 gtttgatggc tactggggca ccaaaccgaa gatcgatacg ctggtcttct ctattactcc
    19261 tgacgcttcc gtgcgttacg cgaaattgca gaagaacgaa tgccaggtga tgccgtaccc
    19321 gaacccggca gatatcgccc gcatgaagca ggataaatcc atcaacctga tggaaatgcc
    19381 ggggctgaac gtcggttatc tctcgtataa cgtgcagaaa aaaccactgg atgacgtgaa
    19441 agttcgccag gctctgacct acgcggtgaa caaagacgcc atcatcaaag cggtttatca
    19501 gggcgcgggc gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga
    19561 cgttcaggac tacacttacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct
    19621 ggaaaaaggt ttctccatcg acctgtgggc aatgccggta caacgtccgt ataacccgaa
    19681 tgctcgccgc atggcggaga tgattcaggc agactgggcg aaagtgggcg tgcaggccaa
    19741 aatcgtcacc tacgaatggg gtgagtacct caagcgtgcg aaagatggcg agcatcagac
    19801 ggtaatgatg ggctggactg gcgataacgg ggatccggat aacttcttcg ccaccctgtt
    19861 cagctgcgcc gcctctgaac aaggctccaa ctactcaaaa tggtgctaca aaccgtttga
    19921 agatctgatt caaccggcgc gtgctaccga cgaccacaat aaacgtgttg aactgtacaa
    19981 acaagcacag gtcgtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt
    20041 gtttgaaccg gtacgcaaag aagttaaagg ctatgtggtt gatccattag gcaaacatca
    20101 cttcgaaaat gtctctatcg aataattaaa gccatacaag actgatggca aaggcaaaac
    20161 tgcctgatgc gctccgctta tcaggcctac gaaaactctg caatgtattg agtttgcacg
    20221 attttgtagg tcggataagg cgtttgcgcc gcatccggca taaacaaagc gctctttgtc
    20281 aacaatctgc atacccggtg gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac
    20341 ttcattccct gtcccacaag ggcgggaatt gatttgtgag caatacagac acgcagttcc
    20401 aggctgcggg tcactataga gaatccgggt tatgttgcag tttattctcc gacgtttggg
    20461 actcgtcatc cccacgttta tcggtattac ccttctcaca tttgcctttg tccacatgat
    20521 cccgggcgat ccggtgatga tcatggcggg cgaacgtggg atctccccag agcgtcacgc
    20581 gcaattgctg gctgaactcg gcttagataa accgatgtgg cagcagtatc tccattacat
    20641 ttggggcgta atgcacggtg atttaggcat ttccatgaaa agccgaattc cggtatggga
    20701 agagttcgtg ccgcgctttc aggccacgct ggaacttggc gtctgcgcga tgatttttgc
    20761 gaccgcagtc gggattccgg ttggtgtgct ggctgcggtt aaacgtggtt ccattttcga
    20821 tcacacagcg gttggcctgg cgctgaccgg ctactcgatg ccaattttct ggtggggcat
    20881 gatgttgatc atgctggttt cggtgcactg gaacctgacg cctgtctccg gtcgcgtgag
    20941 cgacatggtg ttcctcgatg attccaatcc gttaaccggt tttatgctga tcgacaccgc
    21001 catctggggt gaagacggta actttatcga tgccgtcgcc catatgatcc tgcccgccat
    21061 tgtgctgggt actattccgc tggcggtcat tgtgcgtatg acgcgctcct cgatgctgga
    21121 agtgctgggc gaagattata tccgcaccgc gcgtgccaaa ggtcttaccc ggatgcgggt
    21181 gattatcgtc catgcgctgc gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca
    21241 ggtgggaaca ttgctggcgg gggcgattct gactgaaacc atcttctcgt ggccgggtct
    21301 ggggcgctgg ttgattgacg cactgcaacg ccgcgattat ccggtagtgc agggcggcgt
    21361 attgctggtg gcgacgatga ttatcctcgt caacctgctg gtcgatctgc tgtacggcgt
    21421 ggtgaacccg cgtattcgtc ataagaagta agggggcatc atgtcacagg ttactgaaaa
    21481 taaagtgatt agcgcaccgg tgccgatgac cccgttacag gagttctggc actattttaa
    21541 acgcaacaaa ggcgcggttg tcgggctggt ttacgtcgtc atcgtgctgt ttatcgcgat
    21601 ctttgccaac tggattgcac cctataaccc agcggaacag ttccgcgatg cactgcttgc
    21661 cccgcctgcc tggcaggaag gcggcagcat ggcgcacttg ctgggcactg atgacgtggg
    21721 gcgtgatgtg ctgtcgcggc tgatgtacgg cgcgcgcttg tcgctgctgg ttggctgtct
    21781 ggtagtggtg ttatcgctga ttatgggcgt tattctcggc ctgatcgctg gttactttgg
    21841 cggtctggtc gataacatca tcatgcgcgt ggtcgatatc atgctggcgc tgccaagtct
    21901 actgctggca ctggtgctgg tggcgatttt cggcccgtcg attggtaacg ccgcgctggc
    21961 gctaaccttc gttgcgctgc cgcattatgt gcgcttaacc cgcgccgccg tgctggtgga
    22021 agttaaccgc gattacgtca ccgcgtctcg cgtggcgggt gccggggcaa tgcgccagat
    22081 gtttattaac atcttcccga actgccttgc gccgctgatt gttcaggcgt cgctcggttt
    22141 ctctaacgcc attctcgata tggctgctct tggtttcctc ggcatgggcg cgcagccgcc
    22201 aacgccggag tggggcacca tgctctctga cgtgttgcag ttcgcgcaaa gcgcctggtg
    22261 ggtcgtgacc ttcccgggtc tggcgatcct gctgacggtg ctggcattta acctgatggg
    22321 tgacggtctg cgtgacgcgc tcgatcccaa actgaagcag taagaggttc gagatggcgt
    22381 tattaaatgt agataaatta tcggtgcatt tcggcgacga aagcgcaccg ttccgcgctg
    22441 tagaccgcat cagctacagc gtaaaacagg gtgaagtggt cgggattgtg ggtgagtccg
    22501 gctctggtaa atcggtcagt tcgctggcga ttatggggct gattgattat ccgggccgcg
    22561 taatggcgga aaagctggag tttaacggtc aggatttgca gcgtatctca gaaaaagagc
    22621 gccgcaacct ggtgggtgcc gaagtggcaa tgatcttcca ggacccgatg accagcctta
    22681 acccgtgcta caccgtgggt ttccagatta tggaagcgat taaggtgcat caaggcggca
    22741 acaaaagtac ccgccgtcag cgagcgattg acctgctgaa tctggtcggt attcccgatc
    22801 cggcatcgcg tctggatgtt tacccgcatc agctttccgg cggcatgagc cagcgcgtga
    22861 tgatcgccat ggcgattgcc tgtcggccaa aactgctgat tgccgatgaa ccaaccactg
    22921 cgctggacgt gaccattcag gcgcaaatta tcgaactgct gctggagcta cagcagaaag
    22981 agaacatggc gctggtgtta attacccatg acctggcgct ggtggcggaa gcggcacata
    23041 aaattatcgt gatgtatgca ggccaggtag tagaaacggg cgatgcgcac gctatcttcc
    23101 atgcgccgcg tcacccgtat actcaggcat tgctgcgtgc gctgccggaa tttgctcagg
    23161 acaaagaacg tctggcgtcg ttgccaggtg tcgttcccgg caagtacgac cgcccgaacg
    23221 gctgcctgct taacccgcgc tgcccctacg ccactgacaa atgccgtgct gaagaaccgg
    23281 cgctgaatat gctcgctgac gggcgtcagt ccaaatgcca ttacccactt gatgatgccg
    23341 ggaggccgac actatgagta cgcatgaggc caccccgcaa caaccgctgt tgcaggctat
    23401 cgacctgaaa aaacattatc cggtgaagaa aggcatgttc gcgccggaac gtctggtgaa
    23461 ggcgctggat ggcgtttcgt ttaaccttga acgtggcaaa acgctggcag tagtaggtga
    23521 atctggctgc ggtaaatcga ccctaggtcg gttgctgacg atgattgaaa cgcccaccgg
    23581 cggcgaactg tattaccagg ggcaggatct gctcaagcac gatccgcagg cgcagaagct
    23641 acgtcggcag aaaatccaga ttgtcttcca gaacccttac ggttcgctga atccgcgtaa
    23701 aaaagttggg cagattctcg aagagccgct gctgatcaac accagcttaa gcaaagaaca
    23761 gcgtcgggaa aaagccctgt cgatgatggc gaaagtcggc ctgaaaaccg agcattatga
    23821 ccgctatccg catatgttct ccggcggtca gcgtcagcgt atcgccattg cccgaggtct
    23881 gatgctcgac ccggatgtgg tgattgccga tgagccggtt tccgcgctgg acgtctcagt
    23941 gcgtgcgcag gtgctgaatc tgatgatgga tttgcagcag gaactggggc tatcttatgt
    24001 ctttatctcg cacgatctgt cggtggtgga gcacattgct gatgaagtga tggtgatgta
    24061 cctgggccgc tgcgtggaga aggggacgaa ggaccaaatc ttcaataacc cgcgccaccc
    24121 gtacactcag gcgctgctct ccgcgacacc acgcctgaac ccggacgatc gtcgcgaacg
    24181 catcaaactt accggtgaac tgccaagccc gctgaatcca ccgccaggct gcgccttcaa
    24241 cgcccgctgt cgtcggcgct tcggcccctg catccagttg cagccgcagc taaaagacta
    24301 cggcggtcaa ctggtagctt gttttgccgt tgatcaggat gaaaatccgc agcgttaact
    24361 cacaaaaccg gggccatgcg ctccggttta ctcaatcaac ttcaacagtg gcgacaagca
    24421 taacaaaatg ccgtacagca gaatcatcca ggctttaaac ccgcgaagtt tttccagttg
    24481 tgagacttta tagatcagga aaaacggaat gaggcacgac acaatgccat ataacgggct
    24541 tcccaactga aagaacacca gtaccgatac acgaaacgaa acccaaatcg tcaacgtaat
    24601 gacgataaaa gtgcagatcg ccagagtcag cacgcgtgag ttaattttcc tggtatcaat
    24661 aattcggctt aacagattga gaataatgcc tttaatggcc tcgtggaaac cgagataaat
    24721 gccaaagaat gcagtcagta cggcaaagat attcagcacc gtagaggtga tatgaatgat
    24781 atgcccaggg atcacctgcg cggccagtgc cagtgccgag atattttgtt caaaggcaga
    24841 aacggcttct tcgtggctaa ttgagaaggt aaacgaaaag gcaaaaaaca ggatcaccgc
    24901 gatgagcgtg acataactaa ttcggtgggt acgcagcgcg agccgggttg ccagtacttt
    24961 atccgcttcc cgtttacgat aggcaatatt cattgggtta agtacctgaa taaataccgc
    25021 ggaaaagaag caaaatggaa tagtaagcag gacatcgcgg aaaaagtcgg aggcttgcgg
    25081 gaaggcggtt atattggcga aattccagtg cgggatcatc gcaaaaccga acacgacaat
    25141 aatccctact ttgaccacca ccattggtcc ggaaatctta aacagtaatc gttcaccacc
    25201 agacgcaatc gccaccagta cggcgaaaat ggcgacttta taaaatagag attgagaaag
    25261 atcggcatcg gttaaaccga aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag
    25321 agagtagata aaaatcccgt ggataatcat caaaaagtag ataaccccga ggaaaattcc
    25381 ccagttcttc cccaggtaat gactgataat atcggtgtag tcattacagg aatcgctttc
    25441 agaaagggtt tttaaataaa tgtcctgtac tacccaggtg gcaggataag caatgatcgc
    25501 tgcggtaata aatacccaaa ttcccttcaa gccaatttgt actggcatca gcacggttcc
    25561 ggcaccaatc gccatgccta tgcataataa aacccagccg aagtcgtagc gtgtaaacgg
    25621 caatttctga tcgtgtttcg gtagtgtgtt gtgctgcata aagtcctgtc cagaaagaag
    25681 tcggcctcgc aaaaatgaat aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat
    25741 acgcagtatg taaaaaatga ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt
    25801 ctacaaaatt gcgcatttca ctactagtct ggagtcagcc cggatatgag ctgcaactat
    25861 tgcaaataaa gaaattaagc tggaaggcat cacgcaatga ggaagattgc cgggtgctga
    25921 cggcaaaaag caatcgtaaa atcagcggca agccggaagg gttccggtga ggcgcaatgt
    25981 tgcgggggct ttatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc
    26041 aggtatgcac ctgacaacat cacgggggcc attcttgcat ctagacca
//
LOCUS       DACADA010000058        25992 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.059, whole genome shotgun sequence.
ACCESSION   DACADA010000058 DACADA010000000
VERSION     DACADA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.059"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(98..238)
                     /locus_tag="ISB17_002321"
     CDS             complement(98..238)
                     /locus_tag="ISB17_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980250.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            334..1050
                     /gene="arcA"
                     /locus_tag="ISB17_002322"
     CDS             334..1050
                     /gene="arcA"
                     /locus_tag="ISB17_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAP1980251.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1110..2462)
                     /gene="creD"
                     /locus_tag="ISB17_002323"
     CDS             complement(1110..2462)
                     /gene="creD"
                     /locus_tag="ISB17_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAP1980252.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDLRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLVLLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(2520..3944)
                     /gene="creC"
                     /locus_tag="ISB17_002324"
     CDS             complement(2520..3944)
                     /gene="creC"
                     /locus_tag="ISB17_002324"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAP1980253.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3944..4633)
                     /gene="creB"
                     /locus_tag="ISB17_002325"
     CDS             complement(3944..4633)
                     /gene="creB"
                     /locus_tag="ISB17_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAP1980254.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFGVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4646..5119)
                     /gene="creA"
                     /locus_tag="ISB17_002326"
     CDS             complement(4646..5119)
                     /gene="creA"
                     /locus_tag="ISB17_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAP1980255.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5330..6199
                     /gene="robA"
                     /locus_tag="ISB17_002327"
     CDS             5330..6199
                     /gene="robA"
                     /locus_tag="ISB17_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAP1980256.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6196..6843)
                     /gene="gpmB"
                     /locus_tag="ISB17_002328"
     CDS             complement(6196..6843)
                     /gene="gpmB"
                     /locus_tag="ISB17_002328"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAP1980257.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6895..7422
                     /gene="yjjX"
                     /locus_tag="ISB17_002329"
     CDS             6895..7422
                     /gene="yjjX"
                     /locus_tag="ISB17_002329"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAP1980258.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7501..7827)
                     /gene="trpR"
                     /locus_tag="ISB17_002330"
     CDS             complement(7501..7827)
                     /gene="trpR"
                     /locus_tag="ISB17_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAP1980259.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7917..9854)
                     /gene="sltY"
                     /locus_tag="ISB17_002331"
     CDS             complement(7917..9854)
                     /gene="sltY"
                     /locus_tag="ISB17_002331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAP1980260.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10061..11728
                     /gene="ettA"
                     /locus_tag="ISB17_002332"
     CDS             10061..11728
                     /gene="ettA"
                     /locus_tag="ISB17_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAP1980261.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11849..13081)
                     /gene="nadR"
                     /locus_tag="ISB17_002333"
     CDS             complement(11849..13081)
                     /gene="nadR"
                     /locus_tag="ISB17_002333"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAP1980262.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13102..14484)
                     /gene="radA"
                     /locus_tag="ISB17_002334"
     CDS             complement(13102..14484)
                     /gene="radA"
                     /locus_tag="ISB17_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP1980263.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14533..15501)
                     /gene="serB"
                     /locus_tag="ISB17_002335"
     CDS             complement(14533..15501)
                     /gene="serB"
                     /locus_tag="ISB17_002335"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAP1980264.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMSGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15607..16251
                     /locus_tag="ISB17_002336"
     CDS             15607..16251
                     /locus_tag="ISB17_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAP1980265.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16279..17295
                     /gene="lplA"
                     /locus_tag="ISB17_002337"
     CDS             16279..17295
                     /gene="lplA"
                     /locus_tag="ISB17_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAP1980266.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFQEQEKELRELSAWMA
                     GAVR"
     gene            complement(17296..18627)
                     /gene="yjjJ"
                     /locus_tag="ISB17_002338"
     CDS             complement(17296..18627)
                     /gene="yjjJ"
                     /locus_tag="ISB17_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HAP1980267.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVARENRVIRFGK
                     ARATRYVLLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTDDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     EGNYQRWITAQRPAEIPLDQKLTRYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRHLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNIS
                     KSAWLTAIPLAQQFWQTVARDPRISEAFRHIAQEMPEKIRQIEEKVTRMGG"
     gene            complement(18794..19513)
                     /gene="deoD"
                     /locus_tag="ISB17_002339"
     CDS             complement(18794..19513)
                     /gene="deoD"
                     /locus_tag="ISB17_002339"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP1980268.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19570..20793)
                     /gene="deoB"
                     /locus_tag="ISB17_002340"
     CDS             complement(19570..20793)
                     /gene="deoB"
                     /locus_tag="ISB17_002340"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP1980269.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20845..22167)
                     /gene="deoA"
                     /locus_tag="ISB17_002341"
     CDS             complement(20845..22167)
                     /gene="deoA"
                     /locus_tag="ISB17_002341"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAP1980270.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEHRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(22245..23024)
                     /gene="deoC"
                     /locus_tag="ISB17_002342"
     CDS             complement(22245..23024)
                     /gene="deoC"
                     /locus_tag="ISB17_002342"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP1980271.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            23282..24832
                     /locus_tag="ISB17_002343"
     CDS             23282..24832
                     /locus_tag="ISB17_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAP1980272.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNVCECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLIDPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24804..25667
                     /gene="yjjW"
                     /locus_tag="ISB17_002344"
     CDS             24804..25667
                     /gene="yjjW"
                     /locus_tag="ISB17_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAP1980273.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSSGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         6098 a         7210 c         6646 g         6038 t
ORIGIN      
        1 atacgatcat ttcatcaaac tgttaacgtg ctacaattga acttgatata tgtcaacgaa
       61 gcgtagtttt attgggtgtc ctgcccctct tagcctgtta tattgctgtt aaaatggtta
      121 ggatgacagc cgtttttgac actgtcgggt cctgagggaa agtacccacg accaagctaa
      181 tgatgttgtt gacgttgatg gaaagtgcat caagaacgca attacgtact ttagtcatgt
      241 tacgccgatc atgttaattt gcagcatgca tcaggcaggt cagggacttt tgtacttcct
      301 gtttcgattt agttggcaat ttaggtagca aacatgcaga ccccgcacat tcttatcgtt
      361 gaagacgagt tggtaacacg caacacgttg aaaagtattt tcgaagcgga aggctatgat
      421 gttttcgaag cgacagatgg cgcggaaatg catcagatcc tctctgaata tgacatcaac
      481 ctggtgatca tggatatcaa tctgccgggt aagaacggtc ttctgttagc gcgtgaactg
      541 cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc gtgacaacga agtcgataaa
      601 attctcggcc tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa
      661 ctgactattc gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa
      721 gaacgtcgta gcgttgaaag ctacaagttc aatggttggg aactggacat caacagccgt
      781 tcgttgatcg gccctgatgg cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg
      841 cttcacttct gtgaaaaccc aggtaaaatt cagtcccgtg ctgaactgct gaagaaaatg
      901 accggccgtg agctgaaacc gcacgaccgt actgtagacg tgacgatccg ccgtattcgt
      961 aaacatttcg aatctacgcc ggatacgccg gaaatcatcg ccaccattca cggtgaaggt
     1021 tatcgcttct gcggtgatct ggaagattaa tcggctttac caccgtcaaa aaaaacggcg
     1081 ctttttagcg ccgtttttat ttttcaacat tatttccaga tacgtaactc atcgtccatg
     1141 gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacgg
     1201 gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg
     1261 ctatcggcag agttgagcag tccccacatc acaccatcca gcaacaacag cacgagggta
     1321 aacaacatgc tattacgcca gcctttcaat accgcctgca aataaatacc gttcattaac
     1381 gccccaatca gactggcgat tatccatgcc acagtaaaac cggtatgttc agaaagcgcc
     1441 agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt
     1501 aaacgttgcg cggtgagcgt ttcaaaaaca aagaatgcca taaaagtcag cgcaatcagc
     1561 agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg cgtcgtcact
     1621 gcgacgctaa acgcggggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca
     1681 ccgagattat tagcaaacca gctactttgc cattgcgctt gaaaacctga ctcgctaact
     1741 tcccgtttgg ctggtagaaa atcacctaaa aaactgggat gcggccagtt gctggttaag
     1801 gtcatttcgc tattacgccc gccaggcacc acagaaagat cgccggtacc gcttaaattc
     1861 agcgccacat tcagcttcag gttctgcttg cgcaagtccc cttcgggtaa agggatatgc
     1921 acgccctgcc cgccttgctc taacccggtg ccgggttcaa tggtcagcgc cgtaccgtta
     1981 acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat
     2041 ggcttgccta aggtgatatt tggcgcgtcg agttcgctaa gacgcgaaac atcgaaatcg
     2101 gcttttagcg ttaaatcact gtgccagacc tgaccggtat agatgcctat cttgcgttct
     2161 tccacattct gattgccatc aaccatcaat gactcaggta accaaaaatg aataaaactt
     2221 cgtttccgct ccacggtttt atcgtcttcc tgcaccgtat aaagctcggt cacaggaata
     2281 gcgatgagcg gcccaacgag tttttgcggc ccgctggtac tttggcgaat cacatcttcc
     2341 acatcgctac ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc
     2401 aacaacagca atactgcacc aaacaaggtg gtcattttcc agaacagggg ggatttcaac
     2461 atagtcttct cctttgcaat gacagcagga tacgaaggct atgtgggaag aatttgaagc
     2521 tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc caccttcctg cacgttgcgc
     2581 agcgtgactt cgccgttaaa caaacgggcg acctcactga caaacgccaa ccccagaccg
     2641 ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc
     2701 gcgtagtcag gaatcccact accggtatcc agcaccttaa gggtgacgta ttcctgatcc
     2761 acttcggcgc ttagcgttat gcgaccgctc tcgggagtaa aatcgatggc gttatccagt
     2821 aaattcccca gcgcctgctc cagtaacgcc ggttcagcag caacgttaat ctcagtaggc
     2881 ataacatgca aagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg
     2941 cgaaataatg ccgccacatc aacagcagtc agaacgactt cctgacgatt ctccagtctt
     3001 gcctggcgta gtaacgtttc caccagtgcc tgcattcgcg cattttgcgt cagaatgttg
     3061 tcggtaaaac gagccaccac ttccggcggc ggaccttcgc gtaaaatttc cgccgcgcca
     3121 cgaatcgccg ccagtgggct ttttagctca tgagttaacg cataaacata ctgctcaata
     3181 tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcgag tttacgcagc
     3241 tcgctactac cgagatcggg gagaggaacg ggcttattgt cagtgacgga atcagcatag
     3301 cgagtgagcc gggcaataga gcggttgatc caccaaacca tgcctgcgcc aatcaccagt
     3361 gcaatcccca acaaaatggc gctggcccat aaaattcgcc gctcgctacg cttaatgacc
     3421 ggggccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg cgagccgtcc
     3481 ataatcggcg cggcaacata catcacagaa ctttcgggat cggcaggatt ttgcaacgtg
     3541 ctgcgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg cgaataatcc
     3601 tgtccaacgg ctttatttgc cgaatcaaac aatactttgc cctgcgcatc ggtcatatag
     3661 acatgatatt cgttgcgcac tttgttaatg ccaccgatat tggcgcgaaa cgggcgatgt
     3721 tgtagctgat taaacgcctg cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg
     3781 ggacgcgcca gctccgccag caacgttgcg gtgtcgatta acgtcccctc ggttgctctt
     3841 cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc cgccaccgcc
     3901 accagtaaaa aatagcccag caacaaccgc atgccgatac gcattacagg cccctcaggc
     3961 tatatcccat gccgcgatga gtattaatcg gtgaaagatc ggggttgatg gcgcgcagct
     4021 tggcacgcag cgttttaatg tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt
     4081 cccacacgct atccattagt tgctggcggg accagacgcg gcccggcgac ttgagtaacg
     4141 tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct
     4201 gcgccgcagg ttcattcaat tcaaaatgac caatacggat aacgggagac ggcgtcgaga
     4261 acttcttcac ccgacgcagt aaggtgcgca ctctggcgca cacttcgcga ggtgaaaacg
     4321 gtttagccac gtagtcgtca gcgccaattt ccagcccaag caggcgatcg acctcttcac
     4381 ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac tggcggcaca
     4441 attcaaagcc gctgatatca ggcaaaccaa catcgagaat catgacatcg ggaacctgct
     4501 ggcgagcttt atccagcact ggcaagcctc gctcaaagac ctcgacgcca aaaccttcct
     4561 gttgcaacat gtagaccagc gtatcggcta tcccttgctc gtcttctact aaccagaccg
     4621 tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga accgcgctaa
     4681 tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa gcgagcgcgt
     4741 tgcgtttagc gtcataaaag cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt
     4801 ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac agttcaatcg
     4861 gcccgacttg ttgacaagaa atggccgcat cggaggtatc ttctgccagg cccaatcccc
     4921 ctttaatacc accggttttc gcccggctca cataacaggt gacatttttc acatcgggat
     4981 cgtcaaaggc ttcaacaaca attttgtgat ccgggccgat cattttaaat acggtgtcga
     5041 ccgaaccaat ctcttctgca tgagccaatg gccccagcat tatcaggctt aaagaaagga
     5101 ttaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca attagttcgt
     5161 cacggtaaat aacatattga ttaagaacaa aatctcaata cttttatttc cgtcaagccc
     5221 taaaacatac tcgactaaga aaaaaacact gaatgctaaa acagcaaaaa atgctattat
     5281 ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta tggatcaggc
     5341 cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc ccctgtcgct
     5401 cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa tgtttaaaga
     5461 tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt
     5521 cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc gcttcgactc
     5581 tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg cactttaccg
     5641 ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctgcgtctgg gtgaattcac
     5701 tatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg ttacccagag
     5761 ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgct accagttctg
     5821 gcacgatttt ctcggcaacg cgccgaccat tccgccggtg ctctacggcc tgaatgaaac
     5881 gcgtccgagt caggataaag acgatgaaca agaggtattc tataccaccg cgttagccca
     5941 ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg gcggcgaata
     6001 tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc tgacggtata
     6061 tggaacatgc atgccaatgc tcaacctgac gcgccgtaaa ggtcaggata ttgagcgata
     6121 ctacccggca gaagatgcca aagcgggaga tcgcccaatt aatctgcgct gtgaactgct
     6181 gattccgatc cgtcgttaac gctgcagctc atctaatgca ggggcgtcca gatgcgagat
     6241 gtcccctgcg gtttcaacca cccagccaga cgccagccac aggctttcct gataatccac
     6301 gcgcgaaata gaacagttac gcagacgtaa gcggcgctct gcccatgctg gtaatccgag
     6361 aatcgtactc accaggcatc ccagtgcaat accatggctc accagtagcg gtcggcttcc
     6421 ctgcggtaag tcccggcaag attccagtgc cgcattaacg cgatcgctga gctcctgcat
     6481 tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagttgcc gacgccagtt
     6541 ctcttcttct tcggtgagtg aatcgatatt tcttgtttcc agtacaccca tatttaattc
     6601 acgcaggcga gaatcaaata tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt
     6661 gcgccgggtg cgtcctaaat cgctactgat gatatgcgta atgccaagct ctttggcacg
     6721 ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt cagactggcc
     6781 ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt atacctgtaa
     6841 catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct gattatgcac
     6901 caagttgtct gtgcgaccac caatcccgct aaaattcagg ccattctgca ggcatttcac
     6961 gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg
     7021 gaacagccct ttggcagtga ggaaacgcgc gctggcgcac gaaatagggt agccaatgcc
     7081 cgccgtttac ttcctgaggc tgatttttgg gtggcgattg aagctggcat cgatggtgac
     7141 agcactttca gttgggtggt gattgaaaac gccagccagc gcggcgaggc gcgttcggct
     7201 actttgccgt tgcctgcggt gattctggaa aaagtgcgtg aaggtgaggc gcttggtccc
     7261 gtgatgtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
     7321 tttaccgccg ggaaactcac tcgcaccagc gtctatcacc aggcggtgat cctggcgctg
     7381 agtccgtttc ataatgccgt gtatcagcca ttgcaggcct gattaggcgt tcacgccgca
     7441 tccggcattc ggtgcacgat gcctgatgcg acgctgacgc atcttatcag gcctacaaaa
     7501 tcaatcgctt ttcagcaaca cgtcttccag ccactggcgc agctcgacgg gcgcggcttt
     7561 cagactatta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
     7621 acgctggctc atttcgccgc gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
     7681 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
     7741 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
     7801 tgctgaatag ggtgattgtt gggccatagt atgtcgccat tgttagcggg ggaagcaaaa
     7861 tgcctccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgtggatcag
     7921 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
     7981 tagtaagcgt cataagccag cacgtttttc acataaccgc gcgtctcgga gaatggaata
     8041 ctctcgacaa atgccactgc gtcgatacgc cctgcgctgt tgccaagcca ggtccgcacc
     8101 cgccctggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
     8161 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
     8221 ccagggctgc tataaccggg aatagagaac atcttcaccg tatgggtcgc ggtaccaggc
     8281 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcactc
     8341 tcctgacgag caatcgccat cgcatagctt tgcgggatct ccttaccact ggtgtagcgt
     8401 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca cagcttcccg
     8461 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
     8521 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggtg
     8581 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
     8641 gcgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc gccgatgcgt
     8701 tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa aatctctttt
     8761 gcttcagctt cacgtccgcg ttccagcagt aaatccgcct gccagtaacg ccattcatct
     8821 ttctctttcg cttccatagg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
     8881 gtgccaagcg ccattcgcac acggcgttca ataagcgaag tagattgcga gcgcataatc
     8941 gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa acgccaggcg
     9001 acaatatcgc gcagctcctg aatctgatct tcattaagct gctgtgcctg agcaagcgac
     9061 gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa cgccaccgcc
     9121 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
     9181 gggttgttcg ccagtgaaat gattgccgaa gcgatagtct ggtaatcggc aggcatctgc
     9241 cctgccagca ctgttaccag gccggtgtta cccgctttca tcgccagacg gatacgctct
     9301 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
     9361 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
     9421 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
     9481 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
     9541 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
     9601 accgtcaccg ccggttgatt catcaggtca tcagtgatct ggcggtattc caggtaggga
     9661 taaagtgggt aatctttcag tccaggcatc atttgttcga ccacatccat ttgtcgatta
     9721 tcccaggcct gcttaatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
     9781 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacgtgt
     9841 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
     9901 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
     9961 ttttacgggg tgatcgactg gctgggatta gacagggaaa caggctacac ttcgcctttg
    10021 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    10081 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    10141 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    10201 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    10261 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgttcg
    10321 tgagtccatt gaagaagcgg tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt
    10381 gtatgcgctg tatgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    10441 tctggaagag atcattcagg ctcacgacgg tcacaacctg aacgttcagc tggagcgtgc
    10501 ggcggatgcg ctgcgtctgc cggactggga tgcgaaaatc gctaaccttt ccggtggtga
    10561 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    10621 cgaaccgacc aaccacctgg atgcggaatc cgtagcgtgg ctggagcgct tcctgcacga
    10681 cttcgaaggc accgtggtgg cgattaccca cgaccgttac ttcctcgata acgttgcggg
    10741 ctggatcctc gaacttgacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg
    10801 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    10861 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgtc agtcaaaagg
    10921 taaagcacgt ctggcgcgct ttgaagaact caacagcacc gaatatcaga aacgtaacga
    10981 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    11041 caacctgcgt aaatcctatg gtgatcgtct gctgattgac tccctgagct tctcgatccc
    11101 gaaaggagcg atcgtcggga tcatcggtcc gaacggtgcg ggtaaatcga cgctgttccg
    11161 tatgatctct ggtcaggaac agccggacag cggcaccatc actttgggtg aaacagtgaa
    11221 actggcgtcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    11281 agtttccggc gggctggata tcatgaagat cggcaacacc gagatgccga gccgcgccta
    11341 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgcgttggcg aactctccgg
    11401 cggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    11461 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    11521 gctggagttc ccgggctgcg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    11581 cgccacgcac attctggatt accaggatga aggtaaagtt gagttcttcg aaggtaactt
    11641 taccgagtac gaagagtaca agaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    11701 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gtaaacgcct
    11761 tatccggcct acaaaccaag caatttcaat acgttgtgaa tctccaggcc tgataagacg
    11821 cggtaagcgt cgcatcaggc attggttctt acctctgctc ccccatcatc tcccgcacca
    11881 gctccacgca gcgcagaaaa cggctgtcgt aatcgtcctc ttcaacccgc acgaattcga
    11941 tattgttctc ttccagcatc tccaccagca agttctggaa ctctttgcga tccaccgaac
    12001 tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt gttctccagc aggatcacca
    12061 gatcgaaacg gtattcatca atcaacgcct gcacaaacgg atgctcacgc ccttcgtact
    12121 ttttgcagaa cgcctgggta gtgacaaaat cggtgtcgat aaacgccact ttattggcat
    12181 atttcactgc aaaatcaata tattgcgcat gtcccagcgc gattttatcg tagtcggaat
    12241 actgcaacgc gatctcatcg ccaccgaggt gcgaaaagac ataatcacga ccatattccc
    12301 acgcactggt agtgttgaag atattggcaa gtttgtttac cagggtggat ttaccgctcg
    12361 attcaccacc gaggatcgcc acggtacgca caaagaatgg cttcacttcc gtaggaatat
    12421 attcccagta gcggaacggg ttttcgcgga tctgcgcacc gctgatactc ataaaggtac
    12481 gcttcggatc gaccagcacc gtctcgatcc ccagatgttc catatactgt ggcgcatcgg
    12541 cttcttccga ggtgtagatc aggtccggct gaatcccttt ttcagccata aactttttga
    12601 tgccgttgct ccatacatcc cagccgtgtg gatatggctc catgccctct tcattgaaag
    12661 catgaatacg gatatttttc tgatatttaa aggtttgcaa tagccagcgc agacgatccg
    12721 gcaccgtggg ctgctgcgac atggcgctgt cttcgaacaa tgcgcggtcg cgggtatcgt
    12781 caaaacccat aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga
    12841 tatgtccggt atgcagcggg taaaacttac cgaatacgac accgatcgtt ttcttctgcc
    12901 ggggaaattc aagtcccaaa taacggtgca atgcctccag cttttgtgcg ctggggcttt
    12961 taattttggc attcagtaac tggcttaaat accctttggt cataccgctg gcatcagcta
    13021 cctgctgtag cgtgcagccc tgttgcttga tggcagtttt caggtaatca aatgacgaca
    13081 tatctccctc cgtatctctc attataagtc gtcgaacacg ctaagcgcgt cggagagttt
    13141 tttaacgcca aaaatctgca tcccttccgg cgtttttttc ggcacgttag ccgccggaac
    13201 aatcgcccgg cgaaaaccgt gtttcgccgc ttctgagatt cgttcctggc cgctgggcac
    13261 cgggcggatc tcccctgcca gcccgacttc accaaacacc accagatcct gcggtagcgg
    13321 tctgtcacgc aggctggaaa ccatcgccag cagtaacgct aaatcggcac tggtttccgt
    13381 caccttcacg ccaccgacca cgttaacaaa cacatcctga tcggccattt gtagaccacc
    13441 gtgacggtga agcaccgcca gcaggattgc cagacggttt tgctccagcc cgactgccac
    13501 gcggcgtgga ttcgccatca tcgagtgatc gacgagcgcc tgaatctcca ccagcagcgg
    13561 acgcgttcct tcccacacta ccatcactga gctaccggag gtcacttcat cgccgcgact
    13621 taagaaaatt gccgaagggt tgctgacttc acgcagcccc tgctcggtca ttgcaaagac
    13681 acccagctca ttcaccgcgc cgaagcggtt tttatggctg cgtaaggtgc gaaaacggga
    13741 gtcggcatcg ccatccaaaa gcaccgaaca gtcgatacag tgttccagca ctttagggcc
    13801 agccagcgag ccatctttgg ttacgtgccc aaccatgaca atcgccacac cgcgcgtttt
    13861 ggcgaagcgc gtcagataag ccgccgtttc acgcacctgt gccacgctgc caggcgatga
    13921 ttgtacatcc gccatatgca tcacctggat ggagtcaatt accatcagct tcggttgctc
    13981 ttcttcggca atcaggcaga tctgctcgat gctggtttcc gacagcatat taagattgtc
    14041 agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact cttcgccggt
    14101 gacatacagc gttttcatct gctgcgccag tttgcacagc gtttgcagca acagcgtgga
    14161 tttccccgca ccagggttac cgccaatcag aatggcgctt cccggcacca cgccgccgcc
    14221 taatacgcgg tcgaactctt taaatccggt ggaaaaacgc ggcagctctt caaggctgat
    14281 gtcggaaagt ttctggactt ttgccactcc ggcgctaccg gcatagccac tgagacgctc
    14341 gttacgcgcc accgttggcg acgctgcaag acgcacctcg gtgatggtgt tccaggcatg
    14401 acaggcactg cactgcccct gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa
    14461 ggcgcgtttt ggagcttttg ccacgatgta cctcgttaat gctgtgccgc ccgcaggatg
    14521 acgggcggac aattacttct gattcaggct gccagagagg atgcagaata cccccatcag
    14581 gtcagcgtga cggatggtga cttccgtctt ttcattcact tttggcttgg catggtaggc
    14641 aatccccagc cctgccgctt tgatcatcgg caggtcattg gctccatcgc caatcgccac
    14701 ggtctgcgcc agcgggattt catactcctg cgcgaggcgg gtcagggttt tcgctttata
    14761 ttgcgcgtca acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca tgatctccag
    14821 ttcattggct accacggctg tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt
    14881 aaagccgccg gaggcaatcg ccactttcca gcccagcgtt tccagcttga gtaccagctg
    14941 cgttaagcct gacatcagcg gcagattttc acgcacctgt tgcagaatat tggcgtcagc
    15001 gcctttcagc gtcgccacgc ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat
    15061 cgcccgttcg gttacctccg ccaccctctc gcccgttccg gccagtttgg caatttcatc
    15121 aatacattca atctggatgg cggtggaatc catgtccatc accagcaaac ccggcgtgcg
    15181 caggtgtggg attttcccca gcggtgcgac atccagttgc gcttcatggg ccaggcgcgt
    15241 agcccgcgca gtgagtgaac ctgccagacg aatcacctga taatcttcca cgcaccaggc
    15301 ggcaacaatc accatagccg cgcccagttt gctctggtat tgggtcagac gttgtttatc
    15361 cagcccacga ccatacagca gccagccgct acgacctgcg tggtaatcca gtggcatcac
    15421 ttcatcacca cttaatgaaa gaggcagacc aggccataaa gagacatctt caggcaagtc
    15481 gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac
    15541 gcatgaggct accttgtatc cattgcttct ggcaacatta agtctcaaat tttcaaaggg
    15601 tggaagatgg ctcgcacaaa actgaaattc cggctgcatc gggcagtgat tgtcctgttc
    15661 tgtcttgcct tgttagtggc gctgatgcag ggggcgtcat ggtttagtca aaaccaccag
    15721 cgacagcgta atccgcaact ggaagaactg gcccgcaccc tggcgcggca ggtgacgcta
    15781 aacgttgcac cgctgatgcg taccgactca ccggatgaaa aacgcattca ggcgatcctc
    15841 gatcagttaa cggatgaaag ccgcattctc gacgcaggcg tatatgatga acaaggcgat
    15901 cttatcgccc gttctggcga aagcgtcgac gtgcgcgacc ggctggcgct cgacggcaaa
    15961 aaagcgggcg gctattttaa tcagcagatt gtcgaaccca ttgcgggtaa aaacggaccg
    16021 ctcggctatc tgcgcctgac gctcgacacc catacgctcg ccaccgaagc ccaacaggtg
    16081 gataacacca ctaacatttt acgcctgatg ttgctgctct cgctggcaat cggcgtagtg
    16141 ctgacccgca cgctgctaca gggtaaacgc acccgctggc agcaatcgcc cttcctgtta
    16201 accgccagca aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg acccattact
    16261 acaagaaagg aaatcgttat gtccacatta cgcctgctca tctctgactc ttacgacccg
    16321 tggtttaacc tggcggtaga ggagtgtatt tttcgccaga tgcccgccac gcagcgcgtt
    16381 ctgtttctct ggcgcaatgc cgacacggta gtgattggtc gtgcgcagaa cccgtggaaa
    16441 gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt
    16501 ggcggcgcgg tgttccacga tctcggcaat acctgtttta cctttatggc tggcaagccg
    16561 gagtacgata aaactatctc cacgtcgatt gtgctcaatg cgctgaacgc gcttggcgtc
    16621 agcgccgaag cttccgggcg taacgatctg gtggtgaaaa ccgccgaagg cgaccgcaaa
    16681 gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg cactttgctg
    16741 ctcaatgccg accttagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg
    16801 gcgaaaggca ttacctcagt gcgttcccgc gtgaccaatc tgaccgaatt gctaccgggg
    16861 atcacccatg agcaggtttg cgaggccata accgaggcct ttttcgccca ttatggcgag
    16921 cgcgtggaag cggaaatcat ctccccggac aaaacgccag acttgccaaa cttcgccgaa
    16981 acctttgccc gccagagtag ctgggaatgg aacttcggtc aggctccggc attctcgcat
    17041 ctgctggatg aacgctttac ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc
    17101 catatcaccc gcgcccaggt gtttaccgac agtctcaacc ccgcgccgct ggaagccctc
    17161 gccgggcgac tgcaaggctg tctgtaccgc gcggatatgc tgcaacagga gtgcgaagcg
    17221 ctgctggttg acttccagga acaggaaaaa gagctacggg agttgtcggc atggatggca
    17281 ggggcggtgc ggtagttacc cacccatgcg ggtaactttc tcttcgattt gccggatttt
    17341 ttccggcatt tcttgcgcaa tgtggcgaaa cgcctcgctg atacgcggat ctctggcgac
    17401 cgtttgccag aactgctgcg ccagcgggat cgccgttaac catgcgcttt tactgatgtt
    17461 aagatcaaac ttcacctcaa tggcagcatc acgcagcatt ccggcgctgt ttggcgcata
    17521 ggccatcggc agcatgtcgt agacgggtgt cagcgcaaat ggtggttcag ataaataaaa
    17581 tgataaattg cctgcgtgca tatcgctgtt ggcgataagt cgcccaaacg cccagatcac
    17641 ttctgtttgc gccacgctct ggtgagtgac aagttgttgc tcacacaaat gccgcattgc
    17701 ctccggccac gatcccggag aagagataaa ctcactttgc accgcctcca gcgacacaat
    17761 gggcaagcga ccatcgttac ctttgcagtc aaagcgttcc gcttccagga atacctgcct
    17821 gttacttgtt acaagcaccg ttgactcgat ggcgtggatc ccgccgtcac gcaggatttg
    17881 cgcggcaata gattcagcaa ttagcaggtc cccccaacgt tggctgactg cggtttgctg
    17941 tggcacggtg aatttcacca acacatgttt attgcctgac ggcgtttgtg catagcaggt
    18001 aaattttggc tgctcgccgc ccgcagaaga acccacaatt tctccagcca gagcatcgct
    18061 tgccaactgt tcgtaacggg tgagtttttg atccaaagga atttccgcag ggcgttgtgc
    18121 agtaatccat cgctgataat tcccctcccc gaccaaccaa ccgccagtat attcaccgtt
    18181 aaatacggtc agggcgtaga gcacatcttc ttcctgccag agacgtatat catcagtcag
    18241 attcagttgc gcggctaact ttctgcccca cgcgcgccct aaaaagccct gcggtcggag
    18301 atcggtcaaa taccagggca aaccatcaaa ccactgttcg tcgccatctg cgcctgttac
    18361 cagacaactt ccctgtggcc agcacaaccg gatgtcggcg aatttatgcg cctttccggc
    18421 atcgtccacc cgccagacgg gaatacgctc aattccgcga taaggacgca gcagtacata
    18481 tcgcgttgcc cgtgctttac caaagcgaat cacccgattt tctctggcaa caaggcgtga
    18541 gaacgtcgcc tgactgattg ccagacgctg gcgcaattcc ggggcagaac gcggcccttg
    18601 cagtaaaaga tcggtcagtt cgctcattaa tgaatagatt cgtgaataga tacttgaata
    18661 gatttattaa catatttcac tgaaaagtat agcgaaaata ttcaagctgg agagataaat
    18721 gaataaatac taaaaagccg gagcagtctc ccactccggc ttcacacgac aatcgccttt
    18781 cagcaaaaca caattactct ttatcgccca gcagaacgga ttccagcgcg attttgatca
    18841 tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt
    18901 cagacacggt gcagatggtc agggctttcg cgccaaattc tgcagcgacg ccgtagatac
    18961 ccgccgcttc catttccacg ccgaggatgc cgtatttttc catcacgtcg aacatttcgc
    19021 cgtccggaga gtagaacagg tcagcggaga acaggttacc aacgcgagca tcaacgccca
    19081 gtgcttttgc cgcgtctacc gcgttacgca ccatgtcgaa gtcagcgata gcggcaaagt
    19141 catggtcttt aaaacggatg cggttaactt tggaatcggt gcaggcaccc ataccgataa
    19201 cgacgtcgcg cagttttacg tgcggcagaa cagcgccaca ggaacccacg cggataattt
    19261 tcttcacgcc gaaatcagtg atcagttctt tggtgtagat ggagcaggac gggataccca
    19321 taccgtggcc cattacggaa attttgcggc ctttgtaagt accagtgaag cccagcatac
    19381 cgcgaacgtt gttcacttca cgggcatctt caaggaaagt ttcagcaata tacttcgcac
    19441 gcagcgggtc gcctggcatc aaaactacgt cagcaaaatc gcccatttct gcattaatgt
    19501 gtggagtagc catcgtttta tcctttttgt gacatattaa aggcggagtc actgctccgc
    19561 ccaaatccat cagaacatgg ctttgccata ttccatatca gaagtaccaa aatattttgc
    19621 cagagtctgg ccgatatccg cgaaggtttc acgatgaccc agcgaacctg gtttcacttt
    19681 cgggccgtaa accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg tccaggtcgg
    19741 gtcacaaccg tggtcagcag tgagaatcag aatatcgtca tcgcgcagca aagacatcag
    19801 ctccggcaga cggcggtcga acagttccag acccgcagca taaccggcga cgtcgcggcg
    19861 gtggccccag gaagagtcga agtcaacgaa gttggtgaag acgatggtgt tatcacccgc
    19921 ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc agcccggtcg ctttcacttt
    19981 tttggtgata ccgcagttgg catagatgtc tgcaatttta ccgaccgaaa ccacctggcc
    20041 gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa cagccaggtc
    20101 gtgacggtta ccagtacgct ggaagttacc ggctttgtcg ccgataaacg gacgagcgat
    20161 aacacgaccg atgttgtagc cgccgttggt cagctcttca cgggcgattt cgcacagctc
    20221 gtagagttta tccaggccaa aggtttcttc atggcaggca atctggaaca cggagtcagc
    20281 ggaggtatag aaaatcggct tgccggtttt catatgctct tcgcccagtt gatccagaat
    20341 gaccgtaccg gaagagtggc agttaccgag gtaacccggc agattagcgc gttcgaccag
    20401 tttatccagc aactcttgcg ggaagctgtt ttcgtgatcg gagaagtatc cccactcaaa
    20461 cagaaccggc acaccagcaa tttcccagtg accagacggg gtgtctttac cggatgacat
    20521 ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg ccatccattc ccgccggaat
    20581 gaaaccggta gaaccttcgt gtgctttcgc cagcccaaga cgggtcagat ttggcagatt
    20641 gagcgggcct ttacgaccgt ggtcagcttc gcctttggca caagcttctg cgatatgacc
    20701 cagggtgtca gccccgacgt caccaaagcg ttctgcatct tctgtagcgc cgatgccgaa
    20761 tgagtccagc accataataa atgcacgttt catatgttct ccgtactcag tgcttcaaat
    20821 ttaaatgatc ggatcagtat accgttattc gctgatgcgg cgatagacag ttggtgtgct
    20881 ttccggtgct ttatcggcaa gtttaattgc cgctttcacc gctttcgccg cttcctgcca
    20941 gctgttttcg tctttcgcgt ggataactgc cagcggacgc tgaccgtcta cctggtcgcc
    21001 cagacgcgcc atatcagtaa agccgacgct gtaatcgatg gtgtcagatg cctgacggcg
    21061 tccgccgccc attgcaacca ctgccatccc cagtgcgcgg gtatccattt cactgacaaa
    21121 cccttcggta tcagcataga ctgctttcgt cagcatcgct gtcggcagat acttcgcgta
    21181 gttctcaacg aagtcggtcg ggcctttttg tgccgcgacc atacgaccaa agacttctgc
    21241 cgctttaccg ttgtccagca ccgcctgcaa tttcgcgcgc gcttcggcgt catttttcgc
    21301 cagtttgccg gagatcagca tctccacgca cagtgccatc gtgacatcaa acagacgcgg
    21361 gttacgatgt tcacccgtca ggaactgcac cgcttcacga acttcaaccg cgttacctgc
    21421 actggaggcc agtacctgat tcatatcggt gagcagcgcg gtggtgcgca cgccagcgcc
    21481 gttagccacg ccaacaatcg cttcggcaag ggcttcagag agttcgtagg tcggcataaa
    21541 cgcgccgcta cccactttca cgtccatcac cagcgcgtcc agaccttccg ccagtttctt
    21601 cgccagaata gaggcggtga tcagcgggat tgaatccacg gttgcggtaa tatcgcgggt
    21661 cgcgtagaaa cgtttatccg ccggagccag tgagctggtc tggccgataa tcgccacgcc
    21721 gacgtcttta ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga agccagggat
    21781 ggattccagt ttgtcgagcg taccgccagt atgaccgagg ccgcgaccgg agatcatcgg
    21841 aatatagccg ccacaggcag cgaccattgg ccccaacatc agcgaagtca catcgccgac
    21901 accgccggtc gagtgtttat caacaatcgg gccattcaga tgcaggcttt tccagtcgag
    21961 aacggttcct gaatctcgca tcgccatggt cagcgagaca cgctcaggca ttgtcatatc
    22021 gtggaagaaa atggtcatcg cgagggcggc aatctgccct tcggagatag tgttgtcgcg
    22081 aatgccgttg ataaagaaac gaatttcttc atcactcagc gcatgaccat cacgtttttt
    22141 acgaataatt tcttgtgcga gaaacaaggt aacctcccgt ggaaaagaga cgaggtaatc
    22201 ggggaaccgg atgagcatcg ctgcatcatc cggcaaatca agccttagta gctgctggcg
    22261 ctcttaccgt cgccgtggcc cagagctttc agcaggcttg ccagcaagct ggaagcacca
    22321 aagcggtagt gacgcgcatc agcccagtca gcaccgaaca gttcatctgc aatggcgaga
    22381 tatttctgcg catcttccgc agtacgcacg ccgcctgccg gtttgaaacc aacggttttt
    22441 tctacgccca tatcgcggat cacttccatc atgatgcgcg cgctttccgg cgtcgcattc
    22501 acagccactt taccggtaga ggttttgatg aagtcagcac ccgctttgat ggagatttca
    22561 gacgctttac ggatcagcgc ttcgtctttc agttcgccgg tttcaataat cactttcagc
    22621 agtacgttcg ccgcagcgca agcctcttta caggctttca ccaggtcaaa accaacctgc
    22681 tcgttacctg ccatcagcgc gcggtacggg aacaccacgt caacttcatc ggcaccgtag
    22741 gcgattgccg cacgggtttc tgccagcgcg atttcgatgt cgtcgttacc gtgtgggaag
    22801 ttagttaccg tagcgatacg gatttccggg gtgccctgct ctttcagagt tttgcgagca
    22861 atcgggataa agcgaggata gatacagata gcggcggtat tgccgaccgg agttttggcc
    22921 tgatgacaca gtgcgatcac tttctcatcg gtgtcgtcgt cattcagggt ggtcaggtcc
    22981 atcaatttca gtgcacgcag gctgcttgct ttcagatcag tcatttcatt ctcctggctt
    23041 gtcgccaata aaattcacct tgcgagtttg ttagtattct aacatctact ccgcaacaca
    23101 cttcgatgta catcacaatt aaggaaatct acttacaagt ttgcatcact gtaatgcgat
    23161 ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc atcaggatca
    23221 aagtgaacat cacgaaactt cttacaatgg cgcatcacct tcggcataaa aggaaaagaa
    23281 gatgcccact tctcatgaaa atgcactgca acaacgttgc cagcaaattg tcaccagccc
    23341 ggtacttagc ccggagcaga agcgccattt tctggcactg gaagcagaaa acaatctgcc
    23401 ttacccacag ctgcctgccg aagcccgccg cgcgctggat gagggggtaa tctgcgatat
    23461 gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta cccgattacg ctcgttttct
    23521 ggcgcacggt tccgaatggc tggagctgga aggcgcgaaa gaccttgatg acgcactctc
    23581 tctgctgacc attctttacc accatgtacc gtcggtcaca tcgatgccgg tctacctggg
    23641 gcaactggat gcgttgttgc aaccgtatgt tagaattcta acacaagacg agatcgatat
    23701 tcgaataaaa cgtttctggc gttacctcga cagaaccctg ccagacgcct ttatgcatgc
    23761 caatatcggt ccgtctgatt cgcccattac ccgtgcaata ttacgtgcag acgcagagct
    23821 gaagcaggtt tcaccgaacc tgacctttat ctacgatcct gatatcaccc ctgatgacct
    23881 gctgctggaa gtggcgaaaa acgtctgtga atgtagcaaa ccgcacatcg ccaacggtcc
    23941 ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt acaactcact
    24001 gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca ttgccgagcg
    24061 cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc aacagcagat
    24121 cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct ttgagaatag
    24181 cttcctggtg aaagaagggt tgattgatcc tgaacgtttt gtgccgatgt ttggcatgta
    24241 tgggctggcg gaagcggtta acttgctgtg tgaaaaagaa gggattgccg cacgctacgg
    24301 taaagaggcc gccgcaaatg aagtgggtta tcgcattagc gcgcaactgg cggagtttgt
    24361 cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg cacagtcggg
    24421 gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccatatggtg atgagccaga
    24481 tccgattacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt ccggcatcag
    24541 cgacattctg acgctcgacg aaaccatcaa acgtaatccg caggcgctgg tacagctttg
    24601 cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg gtaacgatct
    24661 ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc gcgccgaagg
    24721 ttcacgcacc aacaccacct ggctgggaga agaagccgca cgcaacactc gtattctgga
    24781 acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt aagattatcc
    24841 ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag ggctgcaatc
    24901 tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac tgtggggagt
    24961 gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg gtgtggaacg
    25021 ctgtggtttg cgagcagtgt gatacctgcc tgaagaggtg tccgcaacat gccacgccaa
    25081 tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt ccgcaaagcg gtgctgttta
    25141 ttgaagggat aacggtaagc agcggcgaag ccacgaccca actgccgttt gtagtggcgc
    25201 tgtttactgc tatcaaaaac gatccgcaac tgcgccacct cacctgcctg gtggacagta
    25261 acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgcgac ggcgcaatgc
    25321 tcgatctcaa agcgtggggg agcgaatgtc atcagcaatt aaccggacgc gataatcagc
    25381 agattaagcg cagcatctgt ttgctggcag agcgcggtaa gctggcggaa ctgcgtttgc
    25441 tggtgatccc tggccaggtg gattatttgc aacacatcga agaactggcg gcgtttatca
    25501 agggactcgg cgatgttccg gtacgcctga acgcgtttca tgcccacggc gtgtatggcg
    25561 aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct gatgcgttaa
    25621 aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat gccggatacg
    25681 gcctacggtt cggcacagac ttgtaggcat gataagacgc gacaagcgtc gcatcaggca
    25741 ttatgcgccg actgccggat gcggcgtgaa cgccttatcc ggcctacggt tcggcacaga
    25801 cttgtaggca tgataagacg cgtcagcgtc gcatcaggca ttgtgcgcca actgccggat
    25861 gcggcgtgaa cgccttatcc ggcctacggt tcggcacaga cttgtaggca tgataagacg
    25921 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    25981 cggcctacgg tt
//
LOCUS       DACADA010000059        25858 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.060, whole genome shotgun sequence.
ACCESSION   DACADA010000059 DACADA010000000
VERSION     DACADA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.060"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(122..769)
                     /gene="ychE"
                     /locus_tag="ISB17_002345"
     CDS             complement(122..769)
                     /gene="ychE"
                     /locus_tag="ISB17_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAP1980274.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1246..3921
                     /gene="adhE"
                     /locus_tag="ISB17_002346"
                     /gene_synonym="adhC"
     CDS             1246..3921
                     /gene="adhE"
                     /locus_tag="ISB17_002346"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP1980275.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4046..5093
                     /locus_tag="ISB17_002347"
                     /pseudo
     CDS             4046..5093
                     /locus_tag="ISB17_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5379..5996)
                     /gene="tdk"
                     /locus_tag="ISB17_002348"
     CDS             complement(5379..5996)
                     /gene="tdk"
                     /locus_tag="ISB17_002348"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP1980276.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQAYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            6600..7013
                     /gene="hns"
                     /locus_tag="ISB17_002349"
     CDS             6600..7013
                     /gene="hns"
                     /locus_tag="ISB17_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAP1980277.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7158..8066)
                     /gene="galU"
                     /locus_tag="ISB17_002350"
     CDS             complement(7158..8066)
                     /gene="galU"
                     /locus_tag="ISB17_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP1980278.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8268..9281)
                     /gene="rssB"
                     /locus_tag="ISB17_002351"
     CDS             complement(8268..9281)
                     /gene="rssB"
                     /locus_tag="ISB17_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAP1980279.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9373..10278)
                     /gene="rssA"
                     /locus_tag="ISB17_002352"
     CDS             complement(9373..10278)
                     /gene="rssA"
                     /locus_tag="ISB17_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAP1980280.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10391..10849
                     /locus_tag="ISB17_002353"
     CDS             10391..10849
                     /locus_tag="ISB17_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAP1980281.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10899..11741
                     /gene="purU"
                     /locus_tag="ISB17_002354"
     CDS             10899..11741
                     /gene="purU"
                     /locus_tag="ISB17_002354"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAP1980282.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11901..11985
                     /locus_tag="ISB17_002355"
     tRNA            11901..11985
                     /locus_tag="ISB17_002355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11935..11937,aa:Tyr,seq:gta)
     gene            complement(12002..13093)
                     /locus_tag="ISB17_002356"
     CDS             complement(12002..13093)
                     /locus_tag="ISB17_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HAP1980283.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(13080..14204)
                     /locus_tag="ISB17_002357"
     CDS             complement(13080..14204)
                     /locus_tag="ISB17_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HAP1980284.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(14197..14799)
                     /locus_tag="ISB17_002358"
     CDS             complement(14197..14799)
                     /locus_tag="ISB17_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HAP1980285.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(14796..16082)
                     /locus_tag="ISB17_002359"
     CDS             complement(14796..16082)
                     /locus_tag="ISB17_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HAP1980286.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(16079..16885)
                     /locus_tag="ISB17_002360"
     CDS             complement(16079..16885)
                     /locus_tag="ISB17_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP1980287.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(16863..17879)
                     /locus_tag="ISB17_002361"
     CDS             complement(16863..17879)
                     /locus_tag="ISB17_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1980288.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(17876..18883)
                     /locus_tag="ISB17_002362"
     CDS             complement(17876..18883)
                     /locus_tag="ISB17_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP1980289.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(19011..19688)
                     /gene="narI"
                     /locus_tag="ISB17_002363"
     CDS             complement(19011..19688)
                     /gene="narI"
                     /locus_tag="ISB17_002363"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP1980290.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(19688..20398)
                     /gene="narJ"
                     /locus_tag="ISB17_002364"
     CDS             complement(19688..20398)
                     /gene="narJ"
                     /locus_tag="ISB17_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP1980291.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(20395..21933)
                     /gene="narH"
                     /locus_tag="ISB17_002365"
     CDS             complement(20395..21933)
                     /gene="narH"
                     /locus_tag="ISB17_002365"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP1980292.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(21930..25673)
                     /locus_tag="ISB17_002366"
     CDS             complement(21930..25673)
                     /locus_tag="ISB17_002366"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP1980293.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         6657 a         6855 c         6222 g         6124 t
ORIGIN      
        1 aactgtaatt tgagaagaat atttttacct attccttttg cttttgataa ctgcgcagga
       61 acaaagtaaa aataatgacg aagtaaacgg agcatggcag ctccgtttca tttgaaagga
      121 attaattaag caggccgggg aaaatcccct taataccagt aacgataaat tcaatcccca
      181 acgccatcag caatagcccc ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa
      241 ctcgtaccag ccacggtgcc atgcgaaaca atccccagca acataaagcg aacaatgcaa
      301 tagccacaaa gaaaccaaac agatagctaa tgctgtgata acgcgtaccc cagacgatgg
      361 tagaactgat cgcccctggc cccgccatca acggcaacgc caatggcacc acaccaatgc
      421 tttcacgcac cgcggtttct gatttttctt gcttgttctg tttatcctca ccaagcttgc
      481 cgctgatcat cgacatcgct attgtcacca ccaggatgcc accggcgata cggaatgaat
      541 caattgatat accaaaaagt tgtagaatcg tgtcgccgag aaaaagcgag atccacaaga
      601 taatggccac agacaggttg gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat
      661 aactggtcat gctgataaag acgggaataa tccctaccgg gttgaccagc gcaaataacc
      721 cgatgaaaaa tttgaagtaa acgggaaaat caaaaaaggt ctgaatcacg gttagctccg
      781 aagcaaaagc cggataatgt tagccataaa taaggttgaa aagacgcgct gacaatacgc
      841 cttttgacag catttttcac ctcctaacta cttaaaattg ctatcattcg ttattgttat
      901 ctagttgtgc aaaacatgct aatgtagcca acaaatcata ctacaatttg ttaactgtta
      961 gctataatgg cgaaaagcca tgctgaaagg tgtcagcttt gcaaaaattt gatcttgatc
     1021 acgtaatcag tacccagaag tgagtaatct tgcttacgcc acctggaagt gacgcattag
     1081 agataataac tctaatgttt aaactctttt agtaaatcac agtgagtgtg agcgcgagta
     1141 agcttttgat ttccatcggt taagcaaatc atcaccgcac tgactatact ctcgtattcg
     1201 agcagatgat ttactaaaaa agtttaacat tatcaggaga gcattatggc tgttactaat
     1261 gtcgctgaac ttaacgcact cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt
     1321 ttcactcaag agcaagtaga caaaatcttc cgcgccgccg ctctggctgc tgcagatgct
     1381 cgaatcccac tcgcgaaaat ggccgttgcc gaatccggca tgggtatcgt cgaagataaa
     1441 gtgatcaaaa accactttgc ttctgaatat atctacaacg cctataaaga tgaaaaaacc
     1501 tgtggtgttc tgtctgaaga cgacactttt ggtaccatta ctatcgctga acccatcggt
     1561 attatttgcg gcatcgttcc gaccactaac ccgacttcaa ctgctatctt caaatcactg
     1621 atcagcctga agacccgtaa cgccattatc ttctccccgc acccgcgtgc aaaagatgca
     1681 accaacaaag cggctgatat cgttctgcag gctgctatcg ctgccggtgc tccgaaagat
     1741 ctgatcggct ggatcgatca accttctgtt gagctgtcta acgcactgat gcaccaccca
     1801 gacatcaacc tgatcctcgc gactggtggt ccgggcatgg ttaaagccgc atacagctcc
     1861 ggtaaaccag ctatcggcgt aggcgcgggc aacactccag ttgttatcga tgaaactgct
     1921 gatatcaaac gtgcagttgc atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc
     1981 tgtgcttctg aacagtctgt tgttgttgtt gactctgttt atgacgcagt acgtgaacgt
     2041 ttcgcaaccc acggtggcta tctgttgcag ggtaaagagc tgaaagctgt tcaggacgtt
     2101 atcctgaaaa acggtgcgct gaacgcggct atcgttggtc agccagccta taaaattgct
     2161 gaactggcag gcttctctgt accagaaaac accaagattc tgatcggtga agtaaccgtt
     2221 gttgatgaaa gcgaaccgtt cgcacatgaa aaactgtccc cgactttggc aatgtaccgt
     2281 gctaaggatt tcgaagacgc ggtagaaaaa gcagaaaaac tggttgctat gggcggtatc
     2341 ggtcatacct cttgcctgta cactgaccag gataaccaac cggctcgcgt ttcttacttc
     2401 ggtcagaaaa tgaaaacggc tcgtatcctg attaacaccc cggcttctca gggtggtatc
     2461 ggtgacctgt ataacttcaa actcgcacct tccctgactc tgggttgtgg ttcctggggt
     2521 ggtaactcca tctctgaaaa cgttggtccg aaacacctga tcaacaagaa aaccgttgct
     2581 aagcgagctg aaaacatgtt gtggcacaaa cttccgaaat ctatctactt ccgccgtggc
     2641 tccctgccaa tcgcgctgga tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg
     2701 actgaccgct tcctgttcaa caatggctat gctgatcaga tcacttccgt attgaaagca
     2761 gcaggcgttg aaactgaagt cttcttcgaa gtagaagctg acccgaccct gagcatcgtt
     2821 cgtaaaggtg cagaactggc aaactccttc aaaccagacg tgattatcgc gctgggtggt
     2881 ggttccccga tggacgctgc gaagatcatg tgggttatgt acgaacatcc ggaaactcac
     2941 ttcgaagaac tggcgctgcg ctttatggat atccgtaaac gtatctacaa gttcccgaaa
     3001 atgggtgtga aagcgaaaat gatcgctgtc accaccactt ctggtacagg ttctgaagtc
     3061 actccgtttg cggttgtaac tgacgacact actggtcaga aatatccgct ggcagactat
     3121 gcactgaccc cggatatggc gattgtcgac gccaaccttg ttatggacat gccgaagtcc
     3181 ctgtgtgctt tcggtggtct ggacgcagta actcacgcca tggaagctta tgtttctgta
     3241 ctggcatctg agttctctga tggtcaggct ctgcaggcac tgaaactact gaaagaatat
     3301 ctgccagcgt cctaccacga agggtctaaa aatccggtag cgcgtgaacg tgttcacagt
     3361 gcagcgacta tcgcgggtat cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg
     3421 gcgcacaaac tgggttccca gttccatatt ccgcacggtc tggcaaacgc cctgctgatt
     3481 tgtaacgtta ttcgctacaa cgcgaatgac aacccgacca agcagactgc attcagccag
     3541 tatgaccgtc cgcaggctcg ccgtcgttat gctgaaattg ctgaccacct gggtctgagc
     3601 gcaccgggcg accgtactgc tgctaagatc gagaaactgc tggcatggct ggaaacactg
     3661 aaagctgaac tgggtattcc gaaatctatc cgtgaagctg gcgttcagga agcagacttc
     3721 ctggcgaacg tggataaact gtctgaagat gcattcgatg accagtgcac cggcgctaac
     3781 ccgcgttacc cgctgatctc cgagctgaaa cagattctgc tggatactta ctacggtcgt
     3841 gattatgtag aaggcgaaac tgcagcgaag aaagaagccg ctccggctaa agctgagaaa
     3901 aaagcgaaaa aatccgctta atcagtagcg ctgtctggca acataacggc cccttctggg
     3961 gtaatgccag tcagttaagg atcagttgac cgatccagtg gctgtgtaag aatccggaaa
     4021 cgctcactcg tttccggatt tttttatgct cattggacag gcccttgatt tggtatcccg
     4081 ttacgattct ctgcgtaacc cactgacttc tctgggggat tacctcgacc ccgaactcat
     4141 ctctcgttgc cttgccgaat caggtactgt aacgctacgc aagcgccgtc ttcccctcga
     4201 aatgatggtc tggtgtattg ttggcatggc gcttgagcgt aaagaacctc ttcaccagat
     4261 tgtgaatcgc ctggatatca tgctgccggg caatcgcccc ttcgttgccc ccagtgccgt
     4321 tattcagacc cgccagcgtc tgggaattga ggctgtccgc cgcgtgttca cgaaaacagc
     4381 gcagctctgg cataacacca cgccgcatcc gcactggtgc ggcctgaccc tgctggccat
     4441 cgatggtgtg ttctggcgca caccggatac accagagaac gatgcagcct tcccccgcca
     4501 gacacatgcc gggaacccgg cgctctacct gcaggtcaaa atggtctgcc agatggaact
     4561 gaccagccat ctgctgacgg ctgcagcctt cggcacgatg aagaacagcg aaaatgagct
     4621 tgctgagcaa cttatagaac aaaccggcga taacaccctg acgttaatgg ataaaggtta
     4681 ttactcactg ggactgttaa atacctggag cctggcggga gaacaccgcc actggatgat
     4741 ccctctcaga aagggagcgc aatatgaaga gatcagaaaa ctgggtaaag gcgatcatct
     4801 ggtgaagctg aacaccagcc cgcaggcacg aaaaaagtgg ccgggactgg gaaatgaggt
     4861 gacagtccgc ctgctgaccg tgacgcgcaa aggaaaagtc tgccatctgc tgacgtcgat
     4921 gacggacgcc atgcgcttcc ccgggacgta taccggagct gatgcgcgat cttgcaagta
     4981 tgggacaact agtgaaatta ccgacaagaa gagaaagggc cttcccgaga gtgataaagg
     5041 agaggccctg gaaatactcc acagccccga aaaagagcca gtcagttgct taactgactg
     5101 gcattacccc ttctggggcc gttttttatt ttcccaaagc aacttttcca taaaccggca
     5161 gcattgtcct tcatcatatt tgcgacgatg tataacgcct acacacagcg atattgtact
     5221 ttacaggtta caagtcaatg tcggtgtata agcgctctgt gaggcgtata acggcgttaa
     5281 aaaactgccg agaagggtgt atagcccgga agaagtgcgt aaaacgaact gacagggata
     5341 aaagcgccct gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc
     5401 tgaatagccg ttaatgagcc gacttctaac gcctctttat agtgtttacg gcatacagaa
     5461 acgtagcgtt cattaccacc aataaccacc tgctcacctt cgttataagg tctgcctgct
     5521 tgatcaagac gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca
     5581 accagtttgt cggaccatgc cagtaagtat tgactgccaa taaataattc acctcgaaaa
     5641 tcggtacgta gaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat
     5701 tcatatgctt gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc
     5761 tgctgttcat gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt
     5821 gcaggtgatg acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt
     5881 tctgccgtat atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac
     5941 aatgctgtag acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc
     6001 cacaggccct caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt
     6061 tcagctcaac agtatgcaca gaagtggtat atgcgttctc ccttacgaag ccttgcataa
     6121 tccttctgag ctatcattac aactgcctcg cttgttataa gcgggtaaat gactgccggt
     6181 aactattcac aatctttaac ctgttgcgca tgtaatagcc ctctgttgac ctccaggaga
     6241 tagtgcaata ctaagtccct gctcttattg cgactgttct acttttcatc attcgcttaa
     6301 tagggaattc tcgtaaacac gactaataca gaagactgaa aggtcgtcag cctacgatta
     6361 tctccccata aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc
     6421 ggtgcaaata gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt
     6481 ggcggcacaa aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga
     6541 atttaaggct ctattattac ctcaacaaac caccccaata taagtttgag attactacaa
     6601 tgagcgaagc acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat
     6661 gtacacttga aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc
     6721 gcgaagaaga aagcgcggct gctgctgaag ttgaagagcg cactcgtaaa ctgcagcaat
     6781 atcgcgaaat gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg
     6841 ccgttaaatc tggcaccaaa gctaagcgtg ctcagcgtcc ggcaaaatat agctacgttg
     6901 acgaaaacgg cgaaactaaa acctggactg gccagggccg tactccagct gtaatcaaaa
     6961 aagcaatgga tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg
     7021 tagattgcac atgctaaaaa tcccgccatc ggcgggattt tttattgtcc ggtttaagac
     7081 aatttaataa gaataagcta taaaaaaaac ggcgtcgatt gctcaacacc gtttcgtgga
     7141 aaacaccgat acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat
     7201 tccgtgccaa gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat
     7261 ttattaccgc agtcatggct cttccctttc atatgatagg cttcaaccgt ttctttttcg
     7321 atcagcatat caattgcgtc ggtgagctga atttcatcac cagctccagg aggggttttt
     7381 gccagcaacg gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac
     7441 ggcgcaacat ccgcttttgg tttttcaacc acgccaacca tcggtacgct ttcacccggc
     7501 gctaattcaa cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca
     7561 accatgatct ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc
     7621 tgtgataaat cggattcata ttcatccaga ataacgtcag gcaaaataac agccacaggt
     7681 tcatcaccca ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagcccc
     7741 tgacgaactt gcataatagt cacgtgcggc ggacaaatag actgcacttc atcaagcagt
     7801 tgacgtttta cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt
     7861 tcaatagagt ttttagatga gtgagtaacc agcacaattt cagtaatgcc agccgcaata
     7921 cattcattca cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg
     7981 atggctttcg tcgccggcaa catcctggtt cctaatcccg ctacggggat aacggctttt
     8041 ttgactttcg tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt
     8101 gttcatattt ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa
     8161 aggaggcgag ttagcataat taagacaatt acttatacga atagcgacga tagtaccacc
     8221 agccgacatt agcaggtaat gcaaatttag cccgtgttat cgtttgctca ttctgcagac
     8281 aacatcaagc gcagccgacc accggttccc catatttggc attgccaggc atcgcatcgc
     8341 tggctcaatt gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt
     8401 tgcacctgat gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga
     8461 tttttcagtt cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc
     8521 tgacggagta aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt
     8581 tggtgtgcaa gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca
     8641 agtacgccat tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc
     8701 gaaagtgcgg caatatcaag caccaggccg ggtttgtccg cggcaactaa ttgacgataa
     8761 ttaacccggc attgggaaat cacctgctga accggcggtt gtagttcctg taataatttc
     8821 gccgctgcag cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc
     8881 tcaacgcggg aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga
     8941 ttcagatctt taaccggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca
     9001 atatctgcca tattttcagt ggcagatatc accagaactg gtgtctggtc acctctgttg
     9061 cgtatatgtt ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc
     9121 aggtctggag tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc
     9181 gttgtcgctc ccaatgagga aaaccacgaa tccagaagcg agcgaaatac ctgctcatct
     9241 tcaacaatga gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc
     9301 tttactcaat agtggcatgc tattgccacc tacgcctgtc agaattaact taagtgtaga
     9361 taaaaattct ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcttttc
     9421 cactgccagc tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat
     9481 ttgcgggcaa acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt
     9541 aaggcggttc tccagcacct ggatagaagt ggtcataatc tctgttgccg ttggcgctgt
     9601 caccgcccga cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggcag
     9661 agaatcacca ttctcgctat tttcttcact gacattaaag gagagcaaat cttgttgcat
     9721 caaatgagca tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt
     9781 gagggaaata ggaattgggt taactacagc gccatcaacc agccagtagc cgttatgcgc
     9841 aacgggtgcc attagtcctg gaatactgca tgatgcacga atagcaagat ggagatcgcc
     9901 ttcagtaaac cataattcac gcccagtact taaattggtg gcaacagccg cgaagcgacg
     9961 ggaacaattt tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc
    10021 gccgcgcagt aacccaccgc gctgccagga gagatccatc aggcgtaaaa catcccaata
    10081 actgaaagag gtcacccaat cttccagcgc agataatcga tcgcatgcat aggcagcgcc
    10141 caccagcgaa ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag
    10201 cgcattaatt acgccaatat gcgaccaacc tcttgccgcg ccagatccca gcgccagccc
    10261 tatttttatc tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc
    10321 gctactgctc ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg
    10381 gagattattt gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg
    10441 ccacccttat gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg
    10501 ttactgcgct tttgtgatgc aagacgcaga ttatttaata aagacctggc atccatcttg
    10561 tggagcagcg gcgttacgtg ccgaattgat tgccggattt gcgcatactg aatggttggg
    10621 attgaccgta tttgaacatt gctggcagga cggcggcgat atcggatttg tcagttttgt
    10681 tgctcgattt accgagggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa
    10741 agaaaacggt caatggtact atattgacgg tacacgtccg cagtttggcc gcaacgatcc
    10801 ctgcccttgt ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt
    10861 acggtttagc aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag
    10921 ttctgcgtac tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct
    10981 acaagcacga gttaaatatc gtgcagaaca atgaatttgt agatcatcgt accgggcgct
    11041 tttttatgcg cacggaactg gaagggattt ttaatgattc caccctgctg gcagatctcg
    11101 atagcgcatt gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag
    11161 tgattctggt cactaaagaa gcacattgcc ttggcgattt gctgatgaaa gccaattacg
    11221 gcggcctgga tgtcgaaatc gcggcagtta ttggtaacca cgataattta cgttctctgg
    11281 ttgagcgttt tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc
    11341 acgatcaaaa gatggcggat gccattgatg cttatcaacc tgactatgtg gtgctggcga
    11401 agtatatgcg ggtattaacg ccggaatttg tggctcgctt cccgaataag atcatcaata
    11461 ttcaccattc cttcctgcca gcgtttatcg gcgcacgtcc ttatcaccag gcctatgagc
    11521 gtggcgtgaa gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggtc
    11581 caatcatcat gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc
    11641 gcgcaggtcg tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc
    11701 agcgcgtctt tgtttacggt aatcggacga ttattcttta atcgtcagcc aaaataactg
    11761 gttagcttta atccgttacg gatgaaaatc acgcaaccag ttcatttttc tcaacgtaac
    11821 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
    11881 agtaaaaagc attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat
    11941 ctgccgtcat cgacttcgaa ggttcgaatc cttcccccac caccatcact cgattccatt
    12001 atcaaacctt cggcttctcc cccattcgcc gtaaaaactg ctgctgtagc ctcaacaaat
    12061 ccgctaacgt caacaactga taatccagac cttgctggct ggcaatttgc gcaataatgc
    12121 ggcttagcgc cgaataatgg cgatgatgcc agtgaggaaa cagatgatgg gtaaggtgca
    12181 gattaatccc acccagccag tagccaagcc atgcaggttg cggcgtccaa tcaaaggtgg
    12241 tagcaaaggt atgcgccagt ctgcccacgg gcattttgcc ctcttctggt ggtaactgcg
    12301 tatgtccctt cgcccagtgc gttccaataa tcaacgtcac aaaaatcaat gacgccagca
    12361 gctggctgag cagataagtc agcaggatgg tcataaaact aaaatcagac aaaagccagg
    12421 gaattatcag acacagcgca caatgcgcca gtttcccggc taagaaatat ccccagccag
    12481 caaagcctcg cagggccaga tgtcgcgtga ccggcgtaaa ccctgcgcga tcgagccagt
    12541 ccactaccca gatataccag ggaaaggtga gcgcggcgac cagtggccaa tagcgatgtt
    12601 gctggcgcat aaaaggtcgc caccgttgcc acggcgtctg gcgaagaatg ccattttctg
    12661 ctgtatctgg atcgtaaaat tcaatattgg taaaaccgtg atgaaagcga acgtggcgca
    12721 cccgccagca gtccggatct agccccatag gaaaagcaac ccaccggtta agccgggcat
    12781 tggtcttttt accacgcaaa aacgcatcat gcgaagcgtc atgcaccaca ttgaccgcta
    12841 acaacatggc gcaaaacatc atcgcgagat accacaacag atacagtccc cattgacgtt
    12901 gaaataacgc cagtaagtaa caggagaaac aagcaataat caacaccgcc attttgacga
    12961 atgcccatcc gtcggcatac cgatgatcgc ctgatttcgc cagataaact ttcgtaccgc
    13021 tttgcagttg tttcaccagt tgtggatcat cggcaggaaa agtgattaac ctgtcgggat
    13081 taaacaggtt catactgttt ccttagcgat ttacgtccta cgccgattac aaataacagg
    13141 caatgggcca gcagaatacc cactaatccc ttccagagca gagtactcca tgaacacata
    13201 tagacaataa aatagagcag tgacaacgtc acagagcacc acaaaatcat accccaatgc
    13261 tgcccgtcgc tcatgccccc cagcgcaacg gagcctgccg ccagcagaac aaataatacg
    13321 gcttgctcgc tggtaataaa ctcaatgcca tagccatatt gttgaatgta gccaagcacc
    13381 agcataaaca gcaggatcac ccctgcccct accgcccatg gagaactctg gtagcgacct
    13441 tcaatcgctt tgccaaatga gcaaaagccc attaagcgta aaaatggcag attactctcg
    13501 cgcatcgggt tacaggacaa gcgactattt tttacgccgt agcctacgtc gtattccggc
    13561 ccggcactat acgaaccgaa aagtttatcc cacaacaaaa aggtgccacc gtaattggta
    13621 tcggcatatt gcttttgatt cagatgatgg acgcgatgat gcgaaggcgt gatcagcact
    13681 ttttccagcc agcccagttt cggcgttagt gcgttatggt taaaaaattg ccaactatag
    13741 tgaataatag aaaccgccaa aaagacctgt aacggcacac caagcatcgc cagcagcata
    13801 aaaaacggga ttgaggttaa cgaggagtac cacgagttcc gcacgcctaa tgacaggttg
    13861 tagtgttccc cctgatgatg caccacatgt accgcccaca gcgggcgcat ttcgtgatgc
    13921 cagcgatgta gccagtaaaa gccgaaatcc cacgccagga tagtaaacaa ccacagcagg
    13981 atcaccggca cattatcaaa cagtccaagg ctgaagtgat tgaccaccag gccatagcag
    14041 agcacttcca ggcagcgaaa taaccacagc atcatatgac cggagttgat attaaaaata
    14101 acgtccagcc agtccacttt ttgccgcccg gtgaattgca acacgcccgc ttcggctaac
    14161 accaacagca ccatcgatat gatggggaat aataaatcac tcatattgtg tcttcctttt
    14221 ctcgcatgtt gcgcatatcc aaccgcaaat cgacgccagc aacgcgccgc ctactaaatc
    14281 gataaataaa tgtctgcgta attgcagaat cgaaaaggcg attgctatcg cccagaccac
    14341 gctcattagc gtcagccaac ggttcccctt tttagcaatc gcccataccg ccaacagcgt
    14401 tagcgcggcg tgtaaggagg gaaaacagtt ctggcttgag tcaatggcga tcagcctgtt
    14461 gagtgcgccc gcggatatcc ccatctgatc aaacgaggga taatgcatgg ttgtgggcca
    14521 gagaagataa aatacgccag ccaccagtgc ggaaatttgc attgaacggg ccagccagcg
    14581 caggtggcaa agtggcgtgg ataaataacc taacggcaca atgaaaaaaa acgagagata
    14641 cagccacaca gcggaggggg aaaacgcaat caaattatca attattgacg gtgttaattg
    14701 atatccctca ccttgcaggt agtcgctaaa gttgtagata accccaacag ttccccagcc
    14761 cagcagcatc tgtttcaggc gtagcagtga attgctcatt cccgccccca ctgacgcaca
    14821 atgcgccgcc gcttggctgt aaaagagact ggcacagttt gcagttccag cgaccaaatt
    14881 aattgcccga cagcgatatc ctggcgtata aacaaggcat tcaacgcctc ccggcaatgc
    14941 tccagcatcc acagctcgca atcggcgatt aacgtcaatt gtgctgaccc ggtttgtagc
    15001 aggcgataat cgactgttag cggcagtgtc attgccagca cacgactaca ggcatcggca
    15061 aaaacggtct gaatatcgcc ggatcttgtt ggtagttgca gttggtcatc ctgccgccct
    15121 tcgatacgcg caatcgccat tgttgcactg ccacaggggc atggctgttc gcttgccacc
    15181 agcacatcat ccagtcgata acgcactatc ggctgtgtgg tgcgggtaaa gtctgtgacg
    15241 atcggcacga aacggtgctc atccagccat tgcggttcaa tgtgaacaaa ctcttcgttg
    15301 agatgtaacg ttccacaccg gcaggtggaa gcgagaaagc cttctgtcgc ctgataaatt
    15361 tcccccacat tgtggaacac gttgcgcagt aattcccgat cctgcggctc cagaacttcc
    15421 gccaccgaaa tcactttttt gacctttagc gtcaattcgc cggccatcac ctccagcgcc
    15481 agtgcgcgta atacctgagc tggcgcgaca ataatcgtgg gcgctcgctg ttggagaagt
    15541 ttgagttgcg cctgaaatgg cgctagcaga tcgtaaaaat cgagacttaa ccagcgatta
    15601 ttcacgcttt gatacaggtt gttgtccgcg cgcagaaaca gcgccacccg ctctttggca
    15661 aataaatcat cgggcaacac ttttgccagc acaccggcgg cccacatctg ttgctcgtgc
    15721 gggctaacaa caaaaagtcc acgacgcccg gatgtacctg atgaaagccc aacgctgaac
    15781 ttaccaacgc agggtttgaa atcacgactc tgctcactgc gcattgcaca atcgagaagt
    15841 tcgtcgcgct ttaaacctgc ggtattcatc tcatcgaagt gcgtcatcat taacgcttta
    15901 tccatcattg gccactgatt aaagggcaac gtcagataac gctgaaacca cgggctatga
    15961 gataagacat tttgccggaa ctgatgcagc ctttttgcct gccagttttc cagtgcctcg
    16021 cgatcggtga aatgcagcct gcgggtacgg aagtagcgcc agatcaacat caacgggatc
    16081 ataaatcccc ctcatggcac aaacgaatct ccaccacacc gttttgccaa agctgatgga
    16141 gttgcccaag cgtgtggtag taggcgtggc tgtcggacat aaccagatgc gccagtggtg
    16201 aaggccctcg cagctcccgg tagttagctg gcgcccatgc cgcatcgcca gccagtaaag
    16261 tccagccgtt gtcggttaac acaaacgcgc caatatgtcc ggcagcatgt cctggaagcg
    16321 gcacgagaat aatttcgcca ttgctttctg gcagcaaata gccgcactca aacggtgcca
    16381 gttgcgcggg caaattcacc gatgcgaaac tttcaataaa gcgcagtgag gagtcaaaat
    16441 tctccgggat tagccctggc acaaaggcct ggcgcagcgc agtgacacca cgcagagttc
    16501 ttacttgccg ccagccttca ccagaacaga tgcaggaaac atcagggaaa tcacgcaggc
    16561 cagcaatatg atcagcgtga aaatgcgaca gaataatcgc atcaacatct ttcgctgcga
    16621 ggccggtggc agaaagttgt cgcaccagcg cctgttgcgg atcgaaataa accggcgtga
    16681 cctggcgata aagacgaaac acaccagatt gcgtgtagtg ttcgaaccac gatgaatagc
    16741 ctgtgtccca cagccaccgt ttgtcgccca cctccagcag ccaggcgcgg gcggggaatt
    16801 tgcacacttt cagccctgcc cctttcagcg caatacagct aatatgggta caccagcccg
    16861 tttcaaatgc ggttattttt gccatgttcc ctcagccatt gtcccgtgat tgcaatacct
    16921 tgttccatcg aaaagcgtgg gcgatatccc agctctgtaa tggccttttg cgcactcagc
    16981 gtcatatcga aacagagtgt tccggcgctg tatcgtgtga taacaggttc ctgattaacg
    17041 cacttcccat acagttccag gccacgcgcg atcagagaaa gcagcgacca gggaacagca
    17101 gcaatgcggt aactcaagcc cagttgctgg tgcagcaagg cgtcaagcat caccgatagc
    17161 tgctgcggct ggtgattgct gatgttgtaa atactgcccg agcgtaagcc ttcaacgttc
    17221 gttgccagcc gcatggcata caccacgttt tgcacgaacg tgagatccaa taacgcgtcg
    17281 cccccgcgcg gcaaacgtaa gcaaccgccg ccctgttgca attgctgcat cagacgagga
    17341 acaattacct ggtcatgggg gccaaacaaa ccacggggac gcaaaagaat aaatctcgtg
    17401 gcagaatatc gagaaacagc gccagcaata acctgctcag cggcatattt actgctggca
    17461 taatggctgg agaatgctcg cgcaagataa gtttctggca ggtcatagtg atgttgaaaa
    17521 tcaaagtaaa ccgcaggcgt ggagatatgg ataaaccggg gaatattcag cctgcctgcc
    17581 gcctgtgcca gagtgtgggt cactttgaca ttggcaagcc agaacgccgt tttgtcgccc
    17641 cagggcgagg actttgccgc acagtgccag acggcatcgc agccgtccat caggcgggcg
    17701 cagtcatcct cggttgcctc ggttaaatcg agagccacaa actgtgcgcc tgcgtttgtc
    17761 aggcttctgc caacctcaca atttcgccct gttgccacaa ccgactcacc cgattgcaac
    17821 aaatattgca cggcatttcg cccaaggccg ctggtcgccc ctgttacgag gatcttcacg
    17881 gtaaaagcac catgccagcc agcgttagtc ccgctgccgt gccaatcaac attacaggtt
    17941 tatccgctgt gaaacgaccg ctaatcactg cttcatgcag cgccgtgggg atagaggccg
    18001 cgacctgatt gccgtaaaaa cgatacacat cgatgagcgc agattccggc actcgcagcc
    18061 gtttacgcat atgttcaagg gaaagatgac tggcctgatg cggaacgacc gtgccgatat
    18121 cagacagttg caggctgctt ttttgcagca accgagaaaa ataatcttca ataagcgaag
    18181 aagcaaaacg aaacagcggt ttcccctgca tatgaaaaag aaagtcgcta tcactcacac
    18241 cagagcgagg attacgccgc gtaccgccag cacgaatttc acacagttcg ctgccttcgg
    18301 gatacatttc gatcaggcta gcgataatcc cgctgcgccc atcgccccgc tcaacaatcg
    18361 cgcacgccgc gccatcacca aaaattaatg aagactcttc atgatcccag tcgataccgc
    18421 gtgaagcaat atctgccgaa acgatggcaa tgcgtcgata tgctccacag tttaataatc
    18481 cggcagcgac ttcaagagcg gaaataaagc tcacacagct actgttgata tcaaacccgg
    18541 caacgcctac gggcatggct gcggctttaa gaatatgaat tgcgctacag ggtaatgcct
    18601 gtacggcaat ggccgatgca caaattagca gatcaatatc agaaggattt aaggctgccg
    18661 ctactaacgc attatgtaaa gcctctgccg ccagttcggc ctggctggcg tcgttgcggg
    18721 caaaatagcg ccagcaaata ccggaacgtc gctcaacata gccaactggc ttatttaact
    18781 gaacatccag ttgtttagaa gtgatttgat cagggggtaa ggcgatcccc gtggcgatta
    18841 tttttaacgc cgcggagatc gtcggttgat taggtcgagc cacggtcagt gctccttcac
    18901 tgctccatag gtggaccaat catatagatg aagtacgttt ccctacaatc cgaaaaatcg
    18961 gcagtgtgta accgccgatg tttggtttag ataatgtttg gtttagataa ttagtgacga
    19021 gcgcgcacca gctgatactt acgcgtcaga tactccaccg ggacactcca gatgtgtacc
    19081 agacgcgaga acgggaacag caggaataac gtcatcccca acaccaggtg cagacggaag
    19141 ataaacgcca cgccatcaag atgttgagaa gcgccaccgt ggaaggtcac taccgactgc
    19201 gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcaga gaacggaatg
    19261 gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat atccgctccg
    19321 gtagtcgttg cacgcacgcg tgggctgaac agacggcgtt tcaacaacag cacgccgcca
    19381 atcagacaca gtacgccgct ggcaccacca gcaaacatcg ccattttctg cttcacttca
    19441 atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa gtgaccgaca
    19501 aaaatcccca gaataccgat atggaacagg ttcgacgcca gattcatccc tttgcgatcc
    19561 agcatctggc tggacgccgc gcgccaggta tactgcccgt agtcataacg cagccagcta
    19621 ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa catattcagg
    19681 aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg acggcaccgg
    19741 caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca gcaaagaatt
    19801 taacctgctc ttcttcccag actgcatcca gcgcctgcgg cgtatcgtcg cgcgcttcgt
    19861 cggcaatttt ttccgccact ttgtcgctgt cgattgcggt attcgccagt ttcagcagca
    19921 gatcaaacag cacggcataa cggctttcac gctgttgcag acgcgcactc agcaatgcca
    19981 gaatcggcgc aatatctttc aaaccctcca cggcttcgct ttgcggcagc tgcgccaggt
    20041 actccagata cagcggcaga tggtcgggca attcgcggct gtttaactgc aagccgtgct
    20101 gctcgtactg cgccagcaga tccaccatcg cctggccgcg gtcacgggat tcaccgtgaa
    20161 catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg ctgtactgcg
    20221 cctgggcatc gagcggatcc atcgtcgtta aatcgcgcag gaaaatgccc agcgcatggg
    20281 catcctcttt tggcagattt ttcgacgcgg caatcgcctc aaacatctct tgttgatgct
    20341 gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt tcgatcatgg
    20401 gtgcggctcc gttttgctgg ttacgtcgat ggcatcgata cggcggctgt tgaacagatt
    20461 gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc catttttctc
    20521 cgggaaggct tcccgcgcca gttcacgatg actgctcggc accacaaagc gatcttcgta
    20581 gttggcaata gccagataac gatacatctc ctgtgcctgg gcttcggtca gaccgacctc
    20641 ttccagcgca cgggtatcga ctttaccgtc aacggtttca gcacgtttgt aatgacgcat
    20701 cgccagcata cgtttcagcg cgcgcagtac cggtttggta tcaccggcgg tcagcagatt
    20761 cgccagatac tgtaccggaa tacgcaggct ttcgacgtct ggcagaatgc cgttgctgcc
    20821 cagttcaccc gcgtctgccg cagactgaat cggcgacaga ggcggcacgt accagaccat
    20881 cggcaatgtg cgatattccg gatgcagcgg cagcgccagt ttccattcca tcgccatttt
    20941 ataaactggc gactgctgcg cagcctcaat aacgctcagc ggaataccgt ctttaatcgc
    21001 ctgctcgatg actttcggat cgttcggatc gaggaacaca tccagctgac gctggtagag
    21061 atctttctcg ttctcggtac ttgccgcacg ttcaatcgcg tcggcatcgt acagcagcac
    21121 gccaagataa cggatacgac cgacacaggt ttctgaacat attgtcggct ggccggcttc
    21181 aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt tgaagtagat
    21241 ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat cctggtcgat
    21301 caggacgatg ccgtcttctt cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca
    21361 tgccgggttc agacagtgtt cgcacaggcg cggcaaatac atcatgaagg tgttttcgaa
    21421 ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca gcttgtcaaa
    21481 ctcaccgccc aggtcatctt cccagttcgg ccctttttcg attttcgcca tccgttcgcc
    21541 ggtaatcagc gaacgcggac gggcaatcgg ctgtgatttg ctgccttccg gcgcggtatg
    21601 caggttctga taatcaaaat caaacggctc gtaataatcg tcgatccccg gcagatgcgg
    21661 gttagcgaag attttaccca gcagcatggc acggttaccc atgcgcggct gcagtttgcc
    21721 gttgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt cagtcgggaa
    21781 gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc cttcacggct
    21841 ggtccagacg tttttacagg tcactgaaca ggtgtggcag ccgatgcact tatcgagatt
    21901 cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac ctggtcattg
    21961 ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg
    22021 ttagaaccta cggtgccgta gtagttaaag ccatatgcca gatgggcata gccgccgatc
    22081 atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc acgctgttgg
    22141 gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat catggtcatc
    22201 cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt gctgttgaag
    22261 acttcaatcc agtcgttatc ggcgataccc agctctttgg catcggcttc actcagccag
    22321 accaccggac caccgcgacc taaagtcagc atcaacaggt tgtcgctgta ggtggagtgg
    22381 ataccccact tctggtgtgg cgtcaggaag ttgagcgctt tttccgggtt gccattggat
    22441 ttctggccca tcacttcttt caccgaacgg gtgtcgatcg gcggacgata aaccagcagg
    22501 ctttcaccga aatcacgcat ccattggtga tcctgataca gctgctgacg gccagagagc
    22561 gtacgccacg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca
    22621 tcttcaaggc cagaccatgt cgggctggag atgattttgc gcggctgtgc ctgaatatcg
    22681 cggaagcgaa tcttctcgtc ttctttattc agcgccagat gcgtatggtc acggccggta
    22741 aactcgctca gggcagccca ggcttttaca gctacctgac cgttggtttc cggtgccagc
    22801 gtcaggatca tctctgccgc atcaattgcg gtattgagca tcggctgacc ttttgccgga
    22861 ccttccgctt ttgtgtagtt gagcttacgc agcagatcca tctcgctctg ggtgttccag
    22921 gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga ggtaaagcgt
    22981 tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcggt tttacctggg
    23041 atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggttgcgc cagttcagcg
    23101 gcagagtcgt gctggatagg cagcgtgacg atgtcagttt ctttacccag atgaccaacg
    23161 cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct tttcgcttcc
    23221 caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc cgaagtattc
    23281 atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg
    23341 ctcgacagac ggaagtccag cgtcaccacc agatccagct tgccttccag accgttgtcc
    23401 tgccagtcca cttcttccgg cttcacgccg ccctgctgcc ccaggtcttt gccctggata
    23461 ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc ggaagaaccg
    23521 agcaggttag aacgccagat gaacaggtta cgcgggtggt ttttaccgtt ttccggctgt
    23581 tccgcagcga aacggataga accctctttc agggatttca ccgtatagtc caccggattc
    23641 atcccggctt tttctgcttc gcgagcgata gtcagcgggt tagtgcctaa ctgcggcgca
    23701 gacggcagcc agcccatgcg ttctgcacgg acgttaaagt cgatcaagtg tccggtatag
    23761 cgagatttat ccgccatcgg cgacagcagt tcttccgcag tcacggtttc ataacgccac
    23821 tggctggagt ggttatagaa ataagaggta ctgttcatgt gacgcgccgg acgctgccag
    23881 tcgagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc ctgacctaca
    23941 tagtgcgccc agccgccccc gctctgaccg acacaaccgc agaaaatcag catgttgatc
    24001 agaccacgat agttcatgtc caggtgatac cagtggttca gccctgcgcc gacgataatc
    24061 atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat gcgaataatc
    24121 tggctgcgag aaacgccggt aatctgctct gcccaggcag gggtgtaagc tttcacatcg
    24181 tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta gtttgccagc
    24241 gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg caggcgtttc
    24301 accggcagct tatgcagcag cacgttttcc agttccactt tgttgaagtg ttccgtgcca
    24361 tcgccaccaa agtacgggaa gcccacctca gcgatctcat cctggctacc cagcaggctc
    24421 agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt ccatttgccc
    24481 ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcacc attggtatta
    24541 aaggcgacag ttttccattc cggattgttt tcctggccca gtgcatccac cagatcagca
    24601 gcgcgcagca tacgacccgc agcgtagtaa ccgtcgcgct cttccagcat caccagcatc
    24661 ggcatgtcgg tgtagcggcg cacatagtcg gtgaaatact ggcttgggtt gtcgaggtgg
    24721 aattcacgca gcattacgtg acccattgcc agcgccattg ccgcatcggt gccctgtttc
    24781 ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt gacggcaaca
    24841 gttttggtcc ctttgtaacg cacttcagta aagaagtgag catccggggt acgcgtctgc
    24901 ggcacgtttg aaccccaggc gatgatgtag ctggagttgt accagtcagc agattccggt
    24961 acgtcagttt gctcacccca ggtttgcgga gacgcaggag gcaggtcgca gtaccagtcg
    25021 tagaagctta agcaagtacc gccaatcagc gagagatagc gcgcacccga tgcgtaagaa
    25081 accatcgaca ttgccggaat tggcgagaag ccagcgacac ggtccgggcc gtaatttttg
    25141 atggtgtaaa cgttagatgc ggcgatcagt tcgttcactt cctgccagga agaacgaaca
    25201 aaaccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc ttcaatgata
    25261 gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca cattttcatc
    25321 aggcgtttgc gcatcatcgg gtatttcagg cggttagcgc tgtaaagata ccaggagtag
    25381 ctggcaccgc gcgggcagcc gcgaggttca tggtttggca ggtccgggcg agtacgcgga
    25441 tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat tttccagctg
    25501 caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg ctgccaacgc
    25561 tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg cccatcggca
    25621 aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact catcggtttt
    25681 ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt aagggcaagg
    25741 taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga tgaaaaataa
    25801 agtaaattcc tggaataccc ctttaggata attaacattt atagcataac agactgta
//
LOCUS       DACADA010000060        25742 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.061, whole genome shotgun sequence.
ACCESSION   DACADA010000060 DACADA010000000
VERSION     DACADA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.061"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1161
                     /locus_tag="ISB17_002367"
     CDS             <1..1161
                     /locus_tag="ISB17_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1980294.1"
                     /translation="YQYELAKSLLERLELNVDPYTQLGELGIGHQQLVEIARALSKDT
                     RVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELMEIGDFITIFRDGR
                     FISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTALHPSGGYKLNDV
                     TFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETIDKCHFQARLKKGL
                     ALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESASVKEMILQLAIK
                     VSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAKTDVYQLVGKMAQ
                     QGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIAASTPQD"
     gene            1179..2246
                     /locus_tag="ISB17_002368"
     CDS             1179..2246
                     /locus_tag="ISB17_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1980295.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            2300..3097
                     /locus_tag="ISB17_002369"
     CDS             2300..3097
                     /locus_tag="ISB17_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1980296.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLVHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            3130..3915
                     /locus_tag="ISB17_002370"
     CDS             3130..3915
                     /locus_tag="ISB17_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1980297.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGDGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(4025..4177)
                     /locus_tag="ISB17_002371"
     CDS             complement(4025..4177)
                     /locus_tag="ISB17_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1980298.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(4527..4679)
                     /locus_tag="ISB17_002372"
     CDS             complement(4527..4679)
                     /locus_tag="ISB17_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1980299.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            5122..6240
                     /locus_tag="ISB17_002373"
     CDS             5122..6240
                     /locus_tag="ISB17_002373"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP1980300.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FISLSAASPYMQGTDTRFASSRPNIFSAFPDNGPMQWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            6306..6554
                     /locus_tag="ISB17_002374"
     CDS             6306..6554
                     /locus_tag="ISB17_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAP1980301.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            6619..7002
                     /locus_tag="ISB17_002375"
     CDS             6619..7002
                     /locus_tag="ISB17_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP1980302.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            7081..7734
                     /gene="nfsB"
                     /locus_tag="ISB17_002376"
     CDS             7081..7734
                     /gene="nfsB"
                     /locus_tag="ISB17_002376"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAP1980303.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            7842..9089
                     /locus_tag="ISB17_002377"
     CDS             7842..9089
                     /locus_tag="ISB17_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP1980304.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(9247..10629)
                     /gene="pheP"
                     /locus_tag="ISB17_002378"
     CDS             complement(9247..10629)
                     /gene="pheP"
                     /locus_tag="ISB17_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAP1980305.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(10731..13874)
                     /gene="cusA"
                     /locus_tag="ISB17_002379"
     CDS             complement(10731..13874)
                     /gene="cusA"
                     /locus_tag="ISB17_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAP1980306.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVSDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(13886..15109)
                     /gene="cusB"
                     /locus_tag="ISB17_002380"
     CDS             complement(13886..15109)
                     /gene="cusB"
                     /locus_tag="ISB17_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAP1980307.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEIDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(15125..15457)
                     /gene="cusF"
                     /locus_tag="ISB17_002381"
     CDS             complement(15125..15457)
                     /gene="cusF"
                     /locus_tag="ISB17_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAP1980308.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(15481..16863)
                     /gene="cusC"
                     /locus_tag="ISB17_002382"
     CDS             complement(15481..16863)
                     /gene="cusC"
                     /locus_tag="ISB17_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAP1980309.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDITEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            17020..17703
                     /gene="cusR"
                     /locus_tag="ISB17_002383"
     CDS             17020..17703
                     /gene="cusR"
                     /locus_tag="ISB17_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAP1980310.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            17693..19135
                     /gene="cusS"
                     /locus_tag="ISB17_002384"
     CDS             17693..19135
                     /gene="cusS"
                     /locus_tag="ISB17_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAP1980311.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            19285..21522
                     /gene="nrfB"
                     /locus_tag="ISB17_002385"
     CDS             19285..21522
                     /gene="nrfB"
                     /locus_tag="ISB17_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAP1980312.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            21509..24481
                     /gene="nfrA"
                     /locus_tag="ISB17_002386"
     CDS             21509..24481
                     /gene="nfrA"
                     /locus_tag="ISB17_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAP1980313.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPASLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WSFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            24482..25372
                     /locus_tag="ISB17_002387"
     CDS             24482..25372
                     /locus_tag="ISB17_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAP1980314.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISIFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         6225 a         6799 c         6521 g         6197 t
ORIGIN      
        1 tatcaatatg aattagccaa atctctatta gaaagactgg aactgaatgt tgatccctat
       61 acccaactgg gagagctagg tattggccat cagcaactgg tggagattgc ccgtgccctt
      121 tctaaagata cccgggtact gattatggat gaaccgacat cggccctcag ccagtcggaa
      181 gtaaaagtcc ttttcaatgt gatcgcacaa ctgaagcgcc gtggtgtcac catcatttat
      241 atctcccatc gtctggaaga gttgatggag attggcgatt tcatcaccat tttccgtgat
      301 ggtcgcttta ttagtgaacg cgccgtgagc gatgccagta ttccatggat catcgagcaa
      361 atggtcggtg ataagaaaaa acatttcgat tatcaaccag ctacacaagg tgacactgta
      421 ctggcggtac aagggttaac ggcgctgcat cccagcggtg gttacaaact caatgatgtg
      481 acattcagcc tgcataaagg cgaagttatc ggcatttacg gcttgctggg cgcaggacgt
      541 actgaactat ttaagggatt ggtgggcctg atgccctgtc agagtgggaa gatccagctt
      601 aatggtgaaa ccatcgacaa atgccatttc caggcgcggc tcaaaaaggg gctggcgttg
      661 gtgccagaag atcgccaggg cgaaggcgtc gttcagatga tgtcgattca gtccaatatg
      721 acgctatccg atttcagcct gcaaggtttt cgccgtgcct ggaaatggct gaatccgcaa
      781 aaagagagcg ccagtgtgaa agagatgatc ctgcaactgg cgatcaaagt cagcgatgcc
      841 gaactcccta ttacctcact cagcggcggt aaccagcaaa aagtggtact tggcaaagcg
      901 ttgatgaccc agccacaggt ggtgtttctt gatgaaccca cacgcggcat tgatgttggc
      961 gcaaaaacag atgtctatca gctggtggga aaaatggcac aacaggggct ggcagtcatg
     1021 ttctcttctt cagaactcga tgaagtcatg gcactggcag atcgcattct ggtgatggct
     1081 gatggccgta ttaccgctga tctcccccgg ggcgaagtca cccgagagaa actgattgcg
     1141 gcttcaacac ctcaagatta atcaggctgg aatttcttat gaatcagaaa tatatgatct
     1201 atatgtacct gttgaaggcc agaacgttta tcgctctgct attagtgatc gccttcttta
     1261 gcgtgatggt gcctaacttc ctgacagcct ctaacctgtt gatcatgact caacacgttg
     1321 cgattaccgg gctgctggca atcggaatga cgctggtgat cctgaccggc ggcatcgatc
     1381 tttccgtcgg cgcggtagcg gggatctgcg gaatggttgc cggggctctt ctaacaaacg
     1441 gcctgccatt gtggagcggc aatattattt tcttcaacgt gccggaagtg atcctctgcg
     1501 tggcggtatt tggcatttta gtgggactgg tgaatggcgc ggtaatcacc cgctttggtg
     1561 tcgcgccctt tatctgcacc ctcggtatga tgtacgtcgc tcgtggctct gccctgctgt
     1621 ttaatgacgg cagcacttat cccaatctca acggcatgga aacgctgggc aacacgggtt
     1681 tcgccaccct tggttctggc acgtttttag gcatttatct accaatctgg ctgatgattg
     1741 gctttttgat tttaggctac tggatcacga ctaaaacgcc gctcggtcgt tatatctatg
     1801 ccattggtgg caatgaatct gccgcgcgac tggcaggcgt ccccatcgta aaagttaaag
     1861 tgttcgtcta tgcgttctcc gggttatgcg ccgcatttgt cggtttgatt gttgcctcgc
     1921 agctgcaaac tgctcaccca atgaccggca atatgttcga aatggatgct attggcgcca
     1981 cggttcttgg tggaactgca ctggcgggtg gtcggggacg tgtctctggc tcgattatcg
     2041 gtgcctttgt cattgtcttc ctcgccgatg gcatggtgat gatgggtgtc agtgacttct
     2101 ggcaaatggt cattaaaggc ctcgttatcg tcaccgccgt ggtggtcgat cagttccagc
     2161 aaaaattgca aaacaaagtc attttgatgc gtcgccatga agaaaagatg gccgccgcac
     2221 ctgccgtaaa accgctttcc agttaattaa catcattcca ccgggcatta aattgcccgg
     2281 cttatacagg ggaaaaaata tgcaacgtaa ttttcagggt aagactgtag ttattaccgg
     2341 ggcatgccgg ggaattgggg ccgggatcgc tgaacgcttc gcccgcgatg gcgcaaatct
     2401 ggtgatggtt tctaacgctg aacgcgtaca tgaaactgca gagcaactgc gccaatgtta
     2461 tcaggctgac attttgtcat tagaagtcga tgtgacagat gaagcgcaag tacaggcact
     2521 ctatgaacaa gcggctgcac gttttggcag catcgatgtt tccattcaga atgcaggcat
     2581 tatcaccatc gattattttg atcgaatgcc ggctgctgat ttcgaaaaag tgctggcggt
     2641 gaacactacg gcagtctggc tctgctgccg ggaagcagcc aaatatatgg tgaaacagaa
     2701 tcacggctgc ctgatcaaca cctcttctgg ccagggacgt caggggttta tctatacccc
     2761 acactatgca gccagcaaaa tgggtgtgat cggcattacg caaagcctgg tgcacgagtt
     2821 agcgcagtgg aatatcactg ttaacgcctt ctgcccgggt attatcgaaa gcgaaatgtg
     2881 ggactacaac gaccgcgtat ggggagaaat tctcagtacc gatgacaagc gttatggcaa
     2941 aggcgagctt atggcggaat gggtggaagg tattccgatg aagcgtgccg gtaaacctga
     3001 agatgttgcc ggactggttg ccttcctcgc ctctgacgat gcgcgttatc tcaccggtca
     3061 aaccatcaac atcgacggcg gcctgattat gtcgtaatgc ccctgcgggc tactaagaga
     3121 gatatcaaaa tgatcgcaaa agattttgct caacaattgt ttgatctcca cgatcgcgtc
     3181 gcctttgtca ctggcgcagg tagcggtatc ggacaaatga tcgctctggc tttagccagc
     3241 gcaggcgcac gagtggtgtg ttttgatcta cgggaagatg gcggactcaa agagacggtt
     3301 aatcatatcg aagcacttgg cggacaagcc ttgtattaca cgggcgatgt tcgggaattg
     3361 agagatcttc gcgcagcagt cgctctggca aaaacacatt ttggtcgtct ggatattgcc
     3421 gtaaacgccg ctggaatcgc taacgcaaac ccggcgctgg agatggaaag cgagcaatgg
     3481 caacgcgtta tcgatatcaa cctgactggt gtgtggaatt cctgtaaggc ggaagctgag
     3541 ttaatggtag agaacggaga cggttcgatt ataaatattg cgtcaatgtc cgggattatt
     3601 gtcaatcgtg gtctggaaca ggcccactac aacagttcca aagccggcgt gattcattta
     3661 tctaaaagtc ttgcgatgga atgggtgagt aaggggatcc gcgtgaactc aatcagccca
     3721 ggatataccg cgacaccaat gaatacacga cctgaaatgg tacatcagac tcgggagttt
     3781 gaaagccaga cgccgattca acggatggcg aaagtggaag agatggcggg tccagcgctg
     3841 ttccttgcca gcgatgcggc gtcgttctgc acgggtgtgg atctggtggt cgatggtggc
     3901 tttgtctgct ggtaaaaatg gtttcgcccg cagccatcac tgaccgcggg cgaaagtgta
     3961 aagcaggtgc cttaccaccc tgacctgaca accggatatg cggggattgc tccccgcaca
     4021 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttta
     4081 cggtaaactc acacagcgag tctccgacca gaagcgtaaa tcccagcacc gtcaaacaca
     4141 gcactatgac tgccacaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
     4201 aagggactac catccaaatt gtctggtttg gagtggcagc cccagggttg atagaaataa
     4261 ctcctggggc ttcatctttc cagacctcaa agttaacctg aaaccggaaa gcttcaggca
     4321 cccgccgtta tcttaccctt cctcaacaaa atagctgttt atttatacag taagtcctgt
     4381 ttcggacttc tgttggcaaa actgatcatt gcccttaaaa cgacatcgcc cgcagccatc
     4441 actgaccgcg ggcgaaagtg taaagcaggt gccttaccac cctgacctga caaccggata
     4501 tgcggggatt actccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta
     4561 aactcaatat tacgttcctt caccgtaaac tcacacagcg agtctccgac cagaagcgta
     4621 aatcccagca ccgtcaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg
     4681 tttatattgc ctccatgcga agaagggact accatccaaa ttgttcaggt ttgggtaggt
     4741 agccccaggg ttgatagaaa tatcgtcctg gggcttcatc tttccagacc tcagagtaaa
     4801 cctgaaaccg gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct
     4861 gtttatttat acagtaaatc ctgtttcgga cttctcactc cccatttaat caactgaagc
     4921 tgaacccgtt tttttctgca tgcctcaatc gtcaaaattc ttcgccgcca gccagctctg
     4981 ccataacttg tcacattgca aaatgtagcc aatttgcttc agtcaccatc accaaaaaaa
     5041 gaaacattcc agaacttccg catctcgccc agccgctccc acttctatac tgaatagaaa
     5101 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
     5161 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
     5221 tcatcaatgc tgattgacac ggttaaaaat cagatcacgg ccggagaggt aaagcacgat
     5281 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
     5341 gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag cggcagacca tcatctggaa
     5401 atttgcggcg gtggcacgca tccgtttcag aaatggcagc gtcaggaggt atgcgataac
     5461 gaacgctatc aacgaacgct ggaaaacttt ggctatctca tccagcaggc gaccgttttt
     5521 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
     5581 ttgtcacggt ttgtgccgca ctttatctcc ctttccgccg cgtcgccata tatgcaagga
     5641 acggatacgc gttttgcctc ttcacgaccg aatatttttt ccgcctttcc cgataatggc
     5701 ccgatgcagt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg cctgagttac
     5761 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag cccccatttt
     5821 ggtacggtgg aagttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
     5881 gcgggattaa ttcaggctac cgcccactgg ttactgacgg aacgcccgtt taaacatcag
     5941 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     6001 ggtgtcatta ccgatccgca tactggcgat cgtcggtcac taacggaagc caccttgcgt
     6061 ttactggaaa aaatcgcccc ttctgcacat aaaattggcg catcgagcgc aattgaagca
     6121 ctgcaccgtc aggtcgtcag tggtctgaat gaagcgcagc taatgcgtga tttcgtcgcc
     6181 gatggtggct cgctaattgg gctggtgaaa aagcattgtg agatctgggc cggagaataa
     6241 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     6301 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     6361 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
     6421 tgaccacgtt ccatctgatg gatcttagcc agctttacac gttattgttt tgtctgtggt
     6481 ttttagtgct gggcgctatt gagtattttg ttctacgctt tatctggcga cgctggttct
     6541 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
     6601 tcgcctaaag gaacatttat ggataagcaa tcactgcacg aaacggcgaa acgcctggct
     6661 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     6721 attggcggca agatttttat gctgtcgtcg gagctgcgcg gcgtcccctt tattaatctg
     6781 aagtccgatc cacaaaaatc cctgctaaat cagcaaatat acccaagcat caagccaggg
     6841 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     6901 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     6961 gatcaaaaac gactgcgtcc tgactcaacc gtaaatcaat agcgcatttt tctcgcttac
     7021 catttcatct cgatccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     7081 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
     7141 aaacttaccc cggaacaggc cgagcagatc aaaactctac tgcaatacag cccatccagc
     7201 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     7261 gccaaatccg ctgccgataa ttacgtgttc aacgagcgta aaatgcttga tgcctcgcac
     7321 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     7381 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     7441 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     7501 aaacaggttt atctcaacgt cggtaacttt ctgctgggcg tggcggcgct gggtctggac
     7561 gcggtgccca tcgaaggttt tgacgccgcc atcctcgatg aagaatttgg tctgaaagag
     7621 aaaggctaca ccagtctggt ggttgttccg gtgggtcatc acagcgttga agattttaac
     7681 gctacgctgc cgaaatctcg tctgccgcaa aacattacct taaccgaagt gtaattctcc
     7741 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     7801 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     7861 aggttgaaga tttagcgggt attgagattg atcacaccac ctcgatggtg atgattttcg
     7921 gtattatttt tctgaccgcc gtcgtggtgc atattttttt gcactgggtg gtactgcgga
     7981 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     8041 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     8101 tctggctaca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     8161 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgtgattttg aatctggcgc
     8221 agaaattccc ggcggcatct cagttaccgc tgaaagggat ttttcagggg attaaactga
     8281 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     8341 ttctgatcag tggtcttggt gcaatggctg ccgtgctaat gttggtattt aaagatccga
     8401 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggtgact
     8461 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     8521 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     8581 actcctttaa aaactggagc gggatgtcag cttctggcgg gcgacgtatt aaacgcagta
     8641 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgcctgaata
     8701 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     8761 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     8821 ttcgtgccta tctgaacgaa tatctgcgga atcatccgcg gattcgcaaa gatatgacct
     8881 taatggtacg ccaactggct cctggtgata acggtttacc gcttgagatc tatgcgttta
     8941 ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     9001 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     9061 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     9121 agcggctttc agattgttga catcattttg tcggatgcgg cgtgaacgcc ttatccgacc
     9181 tacagaatcg ttacaaattg taggcccgat aagcttgcgc atcgggcaaa tcagcctcac
     9241 atcagcttaa ttcactttcc tacgcaatgt tttaaacgcc acaaacagga acacaatcca
     9301 caccggcagc agaatcgctg acaagcgcat atcatccatc gtgcacatca gcagcaaaat
     9361 catgccgagg aaggcgatgc aaagatagtt gccgaacgga tagagcagcg ccttaaattg
     9421 tgtttcacgc ccctgacgtc gcatcgccgc gcgaaaacgc agatgcgcca ggcagatcat
     9481 aatccagttc aacagcagcg ttgctaccac cagcgccatc agcagaccaa acgctttttg
     9541 cggcagcaga tagttgatta acaccaccag cgaagtgatc gctccggaaa gcatcagcga
     9601 gttaatcggc acgccgcgac ggctgacgcg agtcaaaaac tttggcgcat taccctgcac
     9661 agaaaggcca aacagcatgc ggctgttaga gtaaaccccg ctgttataca ccgacagcga
     9721 tgccaccaga atgacgaagt tcagcgcaga agctaccacg ttgctgtcga gattatggaa
     9781 aatcatcaca aacgggctac tattggattt cacttccacc cacgggtaga gcgccagtaa
     9841 aaccaccagt gaaccgatat aaaacagcag gatgcgatac accacctgat tcactgcttt
     9901 agggatgctt ttttccggat cgcgcgcttc agccgcagta atcccaatca gctccagacc
     9961 gccgaaggag aacataatta ccgccagcga caaaatcagt ccattccagc cggtggcaaa
    10021 gaaaccgccg tagcgccaga ggttatcgat actggctttc tcaccgccgt gcccagaaaa
    10081 cagcagccac aggccaaagc cgatcatacc gatgatcgcc agcactttaa tcagcgcaaa
    10141 ccagaactcg gtttcgccat ataagcgcac gttcaccagg ttaacggcgt tgatgataat
    10201 aaagaaggcg gcagcccaaa tccacgttgg aacatccggg aaccagtact gcatatagat
    10261 gcccgcagcg gtcagctctg ccattcccac cagcacgaac attacccagt agttccagcc
    10321 agagaggaag cccgcaaacg gtccccagta tttataggca aagtgggcaa atgaaccgga
    10381 taccggctct tcgaccacca tctcgccgag ctggcgcata atcaggaaag cgatgatccc
    10441 ggcgacgccg tagcccagca atacagccgg acccgccatc tgaatcgccg ggccaatgcc
    10501 aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat gacggttatg
    10561 taatccgcga tgaagcgtcg gctcttgatt cgacgcagta tcttccgata cggttgacgc
    10621 gtttttcacg cctttcccct gtgtgtcttt tttgttgagg ggcacctttt aacatttagt
    10681 gcccatcgtc gcaagacaca atccacacgg ttaaacgggg tatcctgctt ttatttccgt
    10741 acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa cagcgacagc
    10801 aaaggcgcgg tgatcatgcc gccaatcatc ggcgcggcaa tccggctcat cacctctgac
    10861 cctgctcctg ttccccacag aatcggcagc agaccagcga taatcaccgc caccgtcatc
    10921 gcttttgggc gcacgcgcag taccgcgccg tgatataacg cctcatccag cttctgctcg
    10981 ctgaatgttt gcggattatt caacgacggc tcggcctcta tggcgtgacg taaatacatc
    11041 agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaaacc ggtgcccgtc
    11101 gccacggaaa gatgaaagcc catccaccac agcaaccaga tgccgcccac cagcgcaaac
    11161 ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaacgccag atacaacagc
    11221 acgaagatga tcatcagcgt catcggcacc atcagtttca gcttatggtt ggcgcgctcc
    11281 agtagctcga actgcccgga gaatgccacg ctggtacccg gttttaactg cactttttca
    11341 gctatcgctt tttgcagatc gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata
    11401 taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc ggtagagact
    11461 ttaacgtcgg ccacgtccgc cagagtgatt tgctgcttca tcggcgtgag gatcggcaac
    11521 tggcgcagtg cctgcgggct atcgcgccag ctttgcggat aacgcagatt aattggataa
    11581 cgagcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga agtcacaaac
    11641 aactgcacat ccgccaccgt cataccgtaa cgcgcggctt tttcacggtt aatctcaacg
    11701 ttgatataac gcccaccttc cagacgctca gcaagcgcag aagccacgcc tggcaccgtt
    11761 cgcgccactt cttcaatttg ctccgccatc gtgtcgatat ccgccagcac agtgccggaa
    11821 actttaatgc cgatagggct tttaatgcca gtcgagagca tatcgatacg gttacgaatt
    11881 ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag ttcctcaatg
    11941 attttgtcca tcgtcatgcc aggccgccac tgatcctgcg gcttaagctg gatagtcgtt
    12001 tctaccatct ccagtggagc tgaatcggtg gcggtttccg ctttcccggt tttgccaaat
    12061 acccgcgcca cttccggtac gctcataatc agcttgtcgt ttttttgcag catgctcgcc
    12121 gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc gccttcattg
    12181 atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt cagcaccgaa
    12241 agcgctgcca ccaacagcgt ggttttcggc cagtgcagta ctttcagcaa cagcggatga
    12301 taaacacgaa tcaaaaagcg attgagcggg ttactgcttt ccggcggaat tttgccacgg
    12361 atccagtagc ccatcaggat cgggatcact acgatcgcca gtagcgccgc gcccgccatc
    12421 gcataggttt tggtgaacgc cagcgggcca aacagacgac cttcctgccc ttccagggtg
    12481 aagatcggga taaacgacag cgtgataatc agcagactga taaacagcgc cggccccact
    12541 tcaacagatg catcggtgat cacctgccag cgcgttttat tatccagcgt ggcgtcagga
    12601 tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac gatagcggca
    12661 tcgaccatcg ccccgacggc aatcgcaatg ccacccaacg acataatatt ggcgttcagt
    12721 ccctgaaagt gcatgacaat aaaagcaata cacaacccca gcggcaacga aataatcgcc
    12781 accagcgccg agcgcacatg ccagagaaac agcgcacaga ctaccgccac cacaataaac
    12841 tcttccagca acttgccgct gaggttgtca atggcgcggt caatgagctg gctgcgatcg
    12901 tatgttgtaa ctatctccac gccttccggc agactgcttt tcagcgtttc cagtttgtcc
    12961 ttcacagcgg cgatcacttc acgggcgttt ttgccggaac gcaggatcac caccccgccc
    13021 gccacttcgc cttcgccgtt cagttcggca atgccccggc gcatctccgg gccaacctgg
    13081 accttcgcaa catcacgcag ataaacgggc acgccatttt cactggcttt taaaacgatg
    13141 tgattaaagt cgtcgagcgt ttgcagatag ccgctggcgc gcaccatata ttccgcttcc
    13201 gccagttcta tcgacgaacc gcccgcttcc tggtttgaag catccagcgc gctttttacg
    13261 tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgctaaccac ctgatactct
    13321 ttcaccacac cgcccaccga cgccacttcc gcaacgtcag ggatggtttt tagctcatat
    13381 ttgagaaacc agtcctgcaa tgagcgtaaa tcggccagat cgtgcttacc gctgcgatcc
    13441 accagtgcat attcatagat ccagccaaca cccgtggcat ctggccccag ctcggcgctg
    13501 actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg cgagcgcgcc
    13561 cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc aaactgcgag
    13621 aaaccgcgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag cggataagtc
    13681 acctgatttt caacgatttg cggtgcctga ccgggatagc tggttttaat aatcacctgc
    13741 acatcggaaa gatccggcag tgcatccact ggcgtgttaa tgatggtcca ggtgccccag
    13801 atactcagaa acaacgcgcc catcagcacc agaaaacggt tcgccaccga gcgacgaata
    13861 atccattcaa tcattggcta ttccctcaat gcgcatgggt agcactttca gagcgcatcc
    13921 gctccagtgc gccagaaata ttggcttcag aatcaatcag gaacaggccg ctggaaacca
    13981 ccttttcacc ttccgccaga ccagagcgta acgcggtgac gccttgcgat gcctggaaaa
    14041 cggcaacgcg tttcggtaca aagcgcccgt cagcatcaac ggtaatcacc cgctgttcgt
    14101 tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg gcggtattga
    14161 gttgcaaccc ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg atttccagac
    14221 gcaactgcag cgtgcgggtc gcggcatcca cgccgggtag cagggtccat ttgcgaatgg
    14281 tgagtgtttt atcaggtcgc gccggaacgg tgagcgtaaa ctgcgaggca tctttcacca
    14341 gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattt
    14401 tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac gcggtgatca
    14461 cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg gcgatcaggc
    14521 ggcgaatatc agcctccggc attcccgcca gtcgcagtcg ctcaagaatg ccttcagtct
    14581 gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct tccacccagt
    14641 cgggaatggt caggtcgaga agcggcgcgc ccttttgcac tttatcgccc acggtaagcg
    14701 gatacacctt gtcgataaac ccggcggcgc gggcctgcac aatggcatac tgatactcgt
    14761 tgtaactgac attcgccggg aaactctggg aaaaagtcag cggcccgcgc gtgacggtcg
    14821 ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac
    14881 tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat ttccctggtt
    14941 tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagacttta cgttctgcgg
    15001 tcgacgtttt ttctgcgggc ggttcctcct ttgcaaacca ggtaaaacct gccgcagaaa
    15061 taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc tcattaaacc
    15121 cggattactg gctgacttta atatcctgta ataaagaaag attgccctgc tggacaaaat
    15181 taaacgctac tttatcgccg gttttaattt cgctcatttt cgtctgcggg gtgatggtaa
    15241 agcgcatagt catctccggc cagttcacgg cagcaatcgg atcgtgatgg atggtgattt
    15301 ttttgctttc cagatcaaca ccttttacca cgccagtggc gctaataacc tgtggttgtg
    15361 cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca ataacggtaa
    15421 acagactgaa cattgcgact tgcagtgctt ttttcattta ttctctcctg gagttaaaaa
    15481 ttattgctgc caaccgcccc ctaaggcggt atataaagaa atttcgttaa cctgacgggc
    15541 ataattcagg tcaagtaaag tttgtcgggt tgcaaataaa gaacgttcag catccagcac
    15601 ttccagataa ctgactgcgc cgtgctgata taatgcccgc gcccgttgca gggtaatttg
    15661 cagcgacgca agataacgct gctgggcgct gatttgatcg ttcaggcttt gacgtaatgc
    15721 cagcgcatct gccacttctt taaaggcgtt ctggattttc tgttcataat tcaccaccga
    15781 ctgctgctgg cgaatttcgg tgatatccag attggcctgg ttgcgtccgg cattaaaaat
    15841 ggggatctca attttaggaa taaaattcca catcccgctg ctggcgttaa ataatgacga
    15901 cagatcgctg ctggcggttg atattccgct ggtcaggctg atagacggga aaaatgccgc
    15961 acgcgcagca ccaatattgg cattagccgc cattaacgcg tgttcagctt ccataatatc
    16021 agggcgctgc aataagattt gcgacgacaa gcccgccggt aatttaacgc tttgcaggct
    16081 gtcgctgttt actgtctgcg cttgcggcag cttgccgtag cttcccaata acagttgcaa
    16141 tgcattattc gcctgcgcca gttccccctg acgtttagcg atgtcgctgc gggtactttc
    16201 tatcaccccg cgagcctgtt ccagcgccag aacattgctg ctaccggtca acagttgttt
    16261 ttcgacaaac gcatatgact gctgataatt acgcagcgtt tcttcggcta tttgcaattg
    16321 cgcatacgcc agttgctgat tgaaatagct ttgcgcgaca ttagaaacca acagaatatg
    16381 caccgcgcgc tgagcttcct cagtggctaa ataattttgg cgctcggctt cgctcatgtt
    16441 ctttaagcga ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg ataactcccg
    16501 cgtcgtggct gaatcgcctt taagattgcc gctccagctg ccgctgccct cgccattgag
    16561 ctgtgggtag cggtcggcat cggtcagacg atattgcgcc cgcgcttcct gcactttcag
    16621 cgccgccatg cgcaaatccc ggttattcac caacgcctcg ctaatcagcg tcttcacctg
    16681 attatcaaca aaaaaggtgc gccagcccgc gttctggtag ttatctgctg cgttaaccag
    16741 gccgttctgg ctgagtgaga actgctgcgg cacgggcatt gccggacgct gataatccgg
    16801 tgccagtgaa caaccggtta gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga
    16861 cataggctca taatttctgg tgattttatg ctgccaactt tactcgccag gctctgattt
    16921 tccggtgaca ggaaaatgac aaaattgtca ttttgccaat aagcgattgc catctgatcc
    16981 cgctactcta gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa
    17041 gatgaaaaga aaaccggaga atacttgacc aaagggttaa ccgaagccgg ttttgtggtc
    17101 gatttggccg acaacgggct gaatggctac catctggcga tgaccggtga ttatgatctg
    17161 ataatcctcg atattatgct gccggacgtg aacggctggg atatcgtgcg catgttgcgt
    17221 tccgccaata aggggatgcc gattctgttg cttaccgcgc ttggcaccat tgaacatcgc
    17281 gtcaaggggc tggagttggg ggcggatgac tatctggtga agccgttcgc ttttgctgaa
    17341 ctgctggcgc gggtgcgcac tctactgcgg cgcggagcgg cggtaattat cgaaagtcag
    17401 tttcaggttg ccgatctgat ggtcgatctc gtcagccgca aagtcacccg cagcggcacg
    17461 cgcatcactc tgaccagtaa agaatttact ctgctggagt ttttcctccg ccatcagggc
    17521 gaagtgctgc cccgctcgct tatcgcctcg caggtatggg acatgaattt tgacagcgat
    17581 accaacgcca ttgatgtggc ggtgaagcgg ctgcgcggca aaatcgacaa cgactttgag
    17641 ccaaagttga ttcagaccgt gcgcggcgtg ggttacatgc tagaggtgcc ggatggtcag
    17701 taagccattt cagcgcccgt tttcgctggc aacccgcctg acctttttta tcagcctcgc
    17761 caccatcgcg gcgtttttcg cctttgcatg gatcatgatc cactcggtaa aagtgcattt
    17821 tgccgagcag gatattaatg atttaaagga gattagcgcc acccttgaac gggtacttaa
    17881 tcaccctgac gaaacgcaag cccgacgctt aatgacgctg gaagatatcg tcagtggtta
    17941 ttccaatgtg ttgatctccc tggcaaatag ccacggtaaa acggtgtatc actcccccgg
    18001 tgcgccggat atccgcgagt ttacgcgtga cgccataccc gataaagacg cgcaggctgg
    18061 cgaggtgtat ctcctttccg gcccgacgat gatgatgcca gggcacggtc acgggcatat
    18121 ggaacacagc aactggcgga tgattaactt gccggttggc ccgctggttg acggcaaacc
    18181 gatttatacg ctctacatcg cgctttcgat cgattttcat cttcattaca tcaatgattt
    18241 gatgaataaa cttattatga ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct
    18301 gttggcggta cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat
    18361 cacctcgaaa gatctcgacg ttcgccttga cccgcagacc gtgcccattg agctggaaca
    18421 gctggtactg tcgttcaacc atatgatcga gcgtattgag gatgtcttta cccgacagtc
    18481 caatttctca gcggatatcg cccacgaaat tcgcacacct attactaatc tcattacgca
    18541 aacggaaatc gccctcagcc agtcgcgcag ccagaaggag ctggaagatg tgctctactc
    18601 taatctcgaa gaactgacgc gaatggcgaa aatggtcagc gatatgctgt ttctcgctca
    18661 ggccgataac aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg
    18721 caaagtgttc gattttttcg aggcgttagc agaagatcgc ggcgtggagc tgcggtttgt
    18781 tggcgacgag tgtcaggttg cgggcgatcc gctgatgctg cgtcgggcgt taagcaacct
    18841 gctttctaat gccctgcgtt atacgccaac cggagagaca attgttgtgc gctgccagac
    18901 ggtcgatcac ctggtgcaag ttaccgtcga aaaccccggt acgcccattg cgcccgaaca
    18961 cttaccgcga ttgtttgacc gtttctaccg cgttgatcct tcccgccagc gaaaaggcga
    19021 aggtagcggc attggcctgg cgatagtgaa atcgattgtt gtcgcgcata aaggcacagt
    19081 tgcggtaacg tcagatgctc gggggacaag gtttgttatc atattacccg cttaatcctt
    19141 cagcaaacgg caacttttat aaccagtgta aaaataacat gccgcaatag taatacctat
    19201 taatgataaa aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa
    19261 ccagcgttaa tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc
    19321 tacggcttaa aagtaatcgc gataacgtta gcggtcatta tgttcatcag cgggctggac
    19381 gattttttta ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat
    19441 cgccgctacc cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg
    19501 attatggttc cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg
    19561 accacgctcg actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat
    19621 actcagcgtg atgttgacga agtgtgcgct cgcttcccga acgtgcataa ggtagtctgc
    19681 gcgcgtcctg gccccaccag caaagccgac tgtctgaaca acgtgctgga cgccatcacc
    19741 caatttgaac gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat
    19801 gtgatttcac caatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt
    19861 cagatcccag tgtatccgtt cgaacgcgaa tggacgcact tcaccagcat gacctacatt
    19921 gatgagtttt cagaactgca tggcaaagat gttccggtgc gtgaagccct cgccggacag
    19981 gtgcccagcg caggagtcgg cacctgtttc agccgccgcg ccgtgaccgc actgttagct
    20041 gacggtgacg gtattgcttt cgacgtgcag agtctgactg aagattacga cattggcttc
    20101 cgcctgaaag agaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc
    20161 aaagaacgcg agcagcgtaa atttttacag catgcgcgga cgtcaaacat gatctgtgtg
    20221 cgcgaatatt tccccgatac cttttcgact gcggttcggc aaaaatctcg ctggatcatc
    20281 ggcattgttt tccagggctt taaaacccac aaatggacct ccagcctgac gctgaactac
    20341 tttctctggc gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg
    20401 gtgatgatcc agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat
    20461 ttcctttcta ttttcagcgg cagcgcatgg ttaatgactc tgctgtggct aaactttggc
    20521 ttgatgatta accgcatcgt gcagcgggtg attttcgtta ctggctacta cggcctgacg
    20581 caggggctgc tatctgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac
    20641 tggcgcgcgt taaaacaggt acttcaacac ggcgatccac gtcgtgtcgc gtgggataaa
    20701 acaacgcatg acttccccag cgtgactggc gatacccgct cgttgcgtcc gttaggtcaa
    20761 atcctgctgg aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc
    20821 gtcgaaggtc tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcaa
    20881 ctggcacagg cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag
    20941 atcccttcct cgctgattgc cgaaatgccg gcctccgtgg cgttgcatta tgcggtactg
    21001 ccgctgcgtc tggacaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt
    21061 tcgctggcgg ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcga
    21121 ggacaaattg tcaccggatt acgccactgg tatgcacgcc gacgcggtca cgatccgcgg
    21181 gcaatgttgt acaatgcagt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc
    21241 tggcggcaat atgtgccgca tcagttcctg ttcgcagaaa tactgaccac gctcggtcat
    21301 attaatcgtt cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc
    21361 ggcaagtttt tggtcaccga aggcgttatt agccaggaaa cgttggatcg cgtcctgaca
    21421 attcaacgcg aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacact
    21481 gaacaggttg cacaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg
    21541 atggtctggt ttactgctga catctttatt gagtaccagc gcactcgcag acaatatcgg
    21601 caccagcgca gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct
    21661 cgataaggcg ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga
    21721 atatatacac cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta
    21781 tcgccatttt ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca
    21841 ccctggagat gaccggcttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt
    21901 tacgactgtt gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg
    21961 ttgtcgcagt gaagtcgggc agaatgccct gcggctggca caattacctg tcgccagagc
    22021 gcaacttaac gatgcgacgt ttgctgcatc gccggaagga aaaacgttgc gaaccgatct
    22081 gctgcaacgg gcaatctacc tgaaacaatg gtcccaggca gatacgctat ataatgaagc
    22141 acgccagcag aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc
    22201 cgcgcagctg gatgatcgga tcctggcgct gcaatcacag gggatcttca ccgatcctca
    22261 gtcatatatt acttatgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca
    22321 ttatctcatt gaaaataagc cgctgtttac cacggacgca caagagaaaa gttggctcta
    22381 tctgttatct aaatacagcg ccaaccccgt tcaggcgttg gcgaattata cggtgcagtt
    22441 tgccgataac cgccagtatg ttgttggcgc gacgctacca gtgctgttaa aagaaggtca
    22501 gtacgacgca gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg
    22561 ttatgccgtt agcgtagcaa cccgtaacaa ggctgaagct ctgcgtctgg cacgattgct
    22621 gtatcagcaa gaaccggcaa gtcttacccg tctggatcaa ctgacctggc aactgatgca
    22681 gaacgggcag tcacgcgaag ctgccgattt attgctgcaa cgctatcctt ttcagggcga
    22741 tgcgcgtgtc agccagacgt taatggcgcg actggcgtct ctgctggaaa gtcatcctta
    22801 cctggcaacg ccggcgaagg tggcaatttt atcgaaacct ttaccgctgg cggagcaacg
    22861 tcagtggcaa agtcagttgc cgggtattgc tgataattgc ccggcaatag ttcgcttgct
    22921 gggcgatatg tcgccttctt acgatgctgc cgcctggaac cgtctggcaa agtgttatcg
    22981 ggacacgcta cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgga
    23041 cgcctggcaa catcgtgcgg tagcctatca ggcgtatcag gttgaggact atgccaccgc
    23101 actgacagcc tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc
    23161 cgccaatacc gcccaggcgg caggaaatgg cgcggctcgc gatcgctggt tacaacaggc
    23221 agaacaacgt ggactgggaa acaatgccct ctactggtgg ctgcatgcgc aacgttacat
    23281 tccaggtcag ccggaactcg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc
    23341 tgccaacgct tacgttgcgc gggcaacaat ttatcgccaa cgtcataata tcccggcggc
    23401 ggtgagtgat ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc
    23461 gcttggttac gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca
    23521 ggcgcataaa gggctaccgg acgatccggc acttatccga caactggcct acgtgaacca
    23581 acgtctggat gacatgcctg cgacgcagca ctacgcccga ctggtgattg atgacattga
    23641 taatcaggcg ctgataaccc cactgacccc agagcaaaat cagcaacgct tcaatttccg
    23701 ccgtctgcat gaggaggtcg gtcgccgctg gtcgttcagt ttcgattctt ctatcggctt
    23761 gcgttccggc gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag
    23821 ctatcgtagc tacgggcaac tggaagccga gtaccgcctc ggacgcaata tgctgctgga
    23881 aggcgacctg ctctcagttt atagccgcgt ctttgccgat accggtgaaa acggggtgat
    23941 gatgccggtg aaaaatccga tgtccggcac cggtctgcgc tggaagccac tgcgcgatca
    24001 gatctttttc ctcgccgtcg aacagcagtt gccgctgaac ggacaaaatg gcgcatccga
    24061 taccatgctg cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca
    24121 cccgaacggt tcaggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg
    24181 ccaggatatt caggcgtgga cggcagatta tcgcgtcagc tggcatcaga aggtggcgaa
    24241 cgggcagact attgagcctt atgctcacgt tcaggacaac ggctatcgtg ataaaggcac
    24301 tcagggcgcg cagcttggcg gtgtcggggt ccgctggaat atctggaccg gcgagacgca
    24361 ctacgacgcc tggccgcaca aagtcagtct cggcgtcgaa tatcaacata cctttaaggc
    24421 gattaatcaa cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta
    24481 aatgcgtaag ttcattttcg tattgctgac actgcttttg gtcagccctt tttcctttgc
    24541 gatgaaaggt attatctggc aaccacaaaa ccgtgatagt caggttaccg atacccagtg
    24601 gcaggggctg atgagtcagt tacgtttgca aggcttcgat acactggttt tgcaatggac
    24661 ccgttacggc gatgcattca cccagccaga acagcgcgcg ttattgttta agcgtgctac
    24721 agctgcgcaa caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt
    24781 tatgcaccag aaacagtcgt ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc
    24841 cgatctccag caagccagat tatggagcgc cgtgcctggc gtaacgccgg atggctggta
    24901 catcagcgcg gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc agcctttgct
    24961 aacatggtta aacaacgcgc agcggttgat tagcgatgtt tcagcaaaac cggtttatat
    25021 cagtattttt ttcgccggaa acatgtcgcc agatggctat cgccaactgc tggaacaagt
    25081 taaagcaacc ggtgttaatg tctgggtaca ggatggcagc ggcgtggata aactgaccgc
    25141 tgaacagcgt gaacgttatc tacaggccag cgccgattgc caaagttccg ccccagcaag
    25201 cggcattgtt tatgaactgt ttgtcgctgg taaaggtaaa acgtttacag cgaaaccgaa
    25261 accggatgca gagatcgcct cgctgttagc gaaacgttcc tcttgcggta aagacacgct
    25321 ctatttctct ctgcgctatt tgcccgtcgc gcagagtatc cttgagtatt aatgattaaa
    25381 gggagcaact tagcttgctc ctttcctttt cactttccta atcaaaatcc tggcaagatg
    25441 aattatttag cagaatatct gttttcattt tattcgtctg acgtgttcca attatttagc
    25501 aaaaccgcga caacaaagtg atggtgtaat ttgtaaaagg gaacaaataa atggctcgcc
    25561 ctgcaggatt cgaacctgta gcccacggct tagaaactcc aggaacgaca ttttaagtca
    25621 acaaattacc gcgccatctc tgcgttcaca cgtcccacta cctcaaaata tgtaaaacct
    25681 tgcaagccat tgcgaggcct tatgtgtctc agttttgttc ctctttttta tactaaaaaa
    25741 ca
//
LOCUS       DACADA010000061        25619 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.062, whole genome shotgun sequence.
ACCESSION   DACADA010000061 DACADA010000000
VERSION     DACADA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.062"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..895)
                     /locus_tag="ISB17_002388"
     CDS             complement(<1..895)
                     /locus_tag="ISB17_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1980315.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEVRQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPL"
     gene            1169..1549
                     /gene="panD"
                     /locus_tag="ISB17_002389"
     CDS             1169..1549
                     /gene="panD"
                     /locus_tag="ISB17_002389"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP1980316.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(1553..2782)
                     /locus_tag="ISB17_002390"
     CDS             complement(1553..2782)
                     /locus_tag="ISB17_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP1980317.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGLGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTQVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(2846..3286)
                     /locus_tag="ISB17_002391"
     CDS             complement(2846..3286)
                     /locus_tag="ISB17_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1980318.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(3391..4161)
                     /locus_tag="ISB17_002392"
     CDS             complement(3391..4161)
                     /locus_tag="ISB17_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1980319.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(4158..5084)
                     /locus_tag="ISB17_002393"
     CDS             complement(4158..5084)
                     /locus_tag="ISB17_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1980320.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            5193..5855
                     /gene="can"
                     /locus_tag="ISB17_002394"
     CDS             5193..5855
                     /gene="can"
                     /locus_tag="ISB17_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAP1980321.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(5896..6432)
                     /gene="hpt"
                     /locus_tag="ISB17_002395"
     CDS             complement(5896..6432)
                     /gene="hpt"
                     /locus_tag="ISB17_002395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP1980322.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            6638..9028
                     /locus_tag="ISB17_002396"
     CDS             6638..9028
                     /locus_tag="ISB17_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HAP1980323.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLSVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(9075..10625)
                     /gene="cueO"
                     /locus_tag="ISB17_002397"
     CDS             complement(9075..10625)
                     /gene="cueO"
                     /locus_tag="ISB17_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAP1980324.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRNLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            10791..11138
                     /locus_tag="ISB17_002398"
     CDS             10791..11138
                     /locus_tag="ISB17_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAP1980325.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            11244..12110
                     /gene="speE"
                     /locus_tag="ISB17_002399"
     CDS             11244..12110
                     /gene="speE"
                     /locus_tag="ISB17_002399"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAP1980326.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            12126..12920
                     /gene="speD"
                     /locus_tag="ISB17_002400"
     CDS             12126..12920
                     /gene="speD"
                     /locus_tag="ISB17_002400"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAP1980327.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(12958..13320)
                     /locus_tag="ISB17_002401"
     CDS             complement(12958..13320)
                     /locus_tag="ISB17_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAP1980328.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(13495..16092)
                     /gene="acnB"
                     /locus_tag="ISB17_002402"
     CDS             complement(13495..16092)
                     /gene="acnB"
                     /locus_tag="ISB17_002402"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAP1980329.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            16447..18210
                     /locus_tag="ISB17_002403"
     CDS             16447..18210
                     /locus_tag="ISB17_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAP1980330.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(18281..19705)
                     /gene="lpdA"
                     /locus_tag="ISB17_002404"
     CDS             complement(18281..19705)
                     /gene="lpdA"
                     /locus_tag="ISB17_002404"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP1980331.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(19913..21805)
                     /gene="aceF"
                     /locus_tag="ISB17_002405"
     CDS             complement(19913..21805)
                     /gene="aceF"
                     /locus_tag="ISB17_002405"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP1980332.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(21820..24483)
                     /gene="aceE"
                     /locus_tag="ISB17_002406"
     CDS             complement(21820..24483)
                     /gene="aceE"
                     /locus_tag="ISB17_002406"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAP1980333.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(24644..25408)
                     /gene="pdhR"
                     /locus_tag="ISB17_002407"
     CDS             complement(24644..25408)
                     /gene="pdhR"
                     /locus_tag="ISB17_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAP1980334.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6109 a         7010 c         6276 g         6224 t
ORIGIN      
        1 caagaggtaa atttgccatc tctgcaacgt cttcccgaga catcccttta ctcagaaaac
       61 gcagggcgaa ttcctgtctt acttcctgtc taacctcttg tattacctcc tgtcttacct
      121 cctgtcttac ctcctgcctt cccctctctt caaaccactg cgccagcgtc atcatagact
      181 cccctcccgt ttccctgtct cgcaacacac cataaaacaa atccgcttgt tcagtatgac
      241 cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt
      301 caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga atatgctttt
      361 gcagtagttc gagaatcgca atccgccggt gctgcatgat ttcgtcatcc ggcgtgatgg
      421 tgatatccac cagcgggaaa gggttgttgt aaacgcgtcg cgccagctcc ggcgagtaaa
      481 acatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagaa
      541 tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcggcaa
      601 tggcatagcg catcatgcga aaggtcattt tcttatccgg cttgctttgg tgttcaatca
      661 caacatgcag ataacccgta ctaccctgca tctgtacgga atagagcacg tcagtgctgt
      721 gccctttcag gctttcttca ataaaactcc ccgactcaag atgcagcgtg tcgaggtcac
      781 acagttcgcg tagctccgct ggcaaatgga tatccagaaa gtcgcgagcc gtttccgcat
      841 gcattaaaaa ttgtttaaac accgcatcat gcggtgtggt actcggtgca tccatctccc
      901 ctgccttccg tgttgtcttg cgtgaaggca gtatccagtc aaggtgtgcc gtcgtcagcc
      961 gcctctttag tgtttaccga gcagcgttca gataaatttc aattggttac aggcgttaca
     1021 tggcacgctt cggcacggta cgcaaactgc tgatgcaaat gccgtcagat gagtagacac
     1081 taaacaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcatc gcctgcttcg
     1141 ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca
     1201 ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgatcagga
     1261 ttttcttgac gcagccggta ttctcgaaaa cgaagccatt gatatctgga atgttaccaa
     1321 cggcaagcgt ttctccacct atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt
     1381 caacggtgcg gcggcccact gcgccagcgt cggcgatatt gtcatcatcg ccagcttcgt
     1441 taccatgcca gatgaagaag ctcgcacctg gcgacccaac gtcgcctatt ttgaaggcga
     1501 caacgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcctgat tgttatccct
     1561 gcggctggtt actcaccagc cgcgacatcg tctccagcga atctgttctt aagatataaa
     1621 gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg gtcgtcacag
     1681 ccaggtgaaa tccggcttct tttgctgcct tcacggcttt gtcattaaat ccgccaaatg
     1741 gataagaaag atacaaaaca tgtggattaa attgcgccag agcgcggcgt gaacgtgcaa
     1801 aatcaaacag aatattgtgc tcactacggc tcagcaatat aggccgccga taaccatcta
     1861 tccgatgcaa aaaatgagta tgtgactgga aatcaaatac atcgcgaatt tcgttaagtt
     1921 cagaaacgct cataaattgc agcgattttg ggttccattt ctgcgggtga cgtttgatgc
     1981 gcgaggtaac aataaacgcc gtcgccttca tgccatattg tttcaacaca ggatacgcat
     2041 agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat
     2101 tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt
     2161 cgcgcagcca ggccatctgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc
     2221 gaaaacgggt gttttcttca tcgcgcaaaa tatggtgata ggttagcacc ggcaggccat
     2281 tatcgggttg ggcatccagc gcgctgatat aggccagtcg atcgccaata cggatctgat
     2341 accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg
     2401 ccagtactcc aaatggcgca ctgcccacac tcggcgcgtt atagacctgc gtatctttcc
     2461 aggtaactaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt
     2521 tttgtcgccc ctgaaccggc tcgagatgcc ctttatcgat aaaccctttg ccaaggccaa
     2581 aattaaatac gtaataactt gcggcagtgg gctccacggc aatgatttgt ccggcccgaa
     2641 tattccccac cgtcaccatc ttgtcaccaa tttgcgccca gaccgcggca ttttcgatgg
     2701 tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga
     2761 gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta
     2821 gcaaaagcgt catcagaatc gcgggttaat actcatacaa attatgtttg aggacgaaag
     2881 gcggattttt ttgttgctgg tgccaaagac tactcacctc cggcgcaccc agttcgacaa
     2941 tacgctcgcg aaacgctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta
     3001 acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt aataatgaag
     3061 ccacgcggta cggtggcgct ccggctatat cggttaagaa aataacgcct tcaccagagt
     3121 ccgtttcatg caaggcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc
     3181 agaaattcac ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta
     3241 tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta
     3301 gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc
     3361 tcccccaaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat
     3421 cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt
     3481 gaccagcgga acatcattga taccgaggaa gccgtagcgg aaaccactga tcatataaac
     3541 gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa
     3601 caccccgccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc
     3661 gaacgtttta gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgcagtgag
     3721 caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga
     3781 aattgccgtc accagaatac caacaaacag accacgcgcc acgccaccac cgacatatcc
     3841 ggcaataatg acatgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa
     3901 cttggcacca aaaaatgatg acgcgacgtt agcgtaggcg ttggtgatca ccgacatcat
     3961 gatcagcccc ggtacgatga actgcatgta gctgaagcca tgcatatcgc caatacgcga
     4021 accaatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag
     4081 cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc
     4141 cacccagtaa agatgcatca tgtgcgatct ccttgttttt cattaaccag tgaaacaaac
     4201 agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt
     4261 aactgcgtaa atacgctgtt gatgccctgc tcacgcagca cttcaacttc cagcgtggcg
     4321 gtatctacca gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga
     4381 tcgagaataa aggtttctga tttcagcttc gccagcagcg ccttcatcga agtattttcc
     4441 accaactcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg
     4501 tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac
     4561 attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta
     4621 ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata
     4681 cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt taagatactt ttcgctgcga
     4741 atgtacgctt ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc
     4801 acggtttcaa acgggttgaa gttaaattcc tgtggcacca gacccaactg acgtttagcg
     4861 tttacgacat ctttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt
     4921 accagagagc tgataatacc gatagtggtc gattttccgg ccccgttcgg cccgagaagc
     4981 gcataaaaat cacccgcttc gacctgcaaa tcgattccac gaagcgcctg aacgccgcct
     5041 ggataggttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct
     5101 tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa
     5161 cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca
     5221 acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg
     5281 cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag
     5341 aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg
     5401 tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactcgaag
     5461 ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa
     5521 acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac
     5581 atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga
     5641 acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac
     5701 gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc ttgctgcgtg
     5761 atctggatgt cacagcgact aaccgcgaaa cccttgagca acgttaccgt cacgggatct
     5821 ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat
     5881 ccggcaactt cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg
     5941 gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc
     6001 gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat
     6061 cgccaacgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc
     6121 cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc
     6181 tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat
     6241 aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga
     6301 gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat
     6361 ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac
     6421 agtatgtttc atatctataa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt
     6481 ttggcaccgc aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa
     6541 tctgttaata aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg
     6601 ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca ataccggctc
     6661 gcggcgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact
     6721 cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct
     6781 tgtgatgctc agcgtcgcct ggatgctgtg gcgcagtaaa cgcgccgcgc tttggctgta
     6841 cgcagccctg ctgctcggca ctatgatttg gggcgtctgg gaagttggtt tcgacttctg
     6901 ggcgctgact ccgcgcagcg acattctggt cttcttcggc atctggctga tcctgccgtt
     6961 tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtcgcact
     7021 gctgattagc ggtggtatcc tgacctgggc cggatttaac gatccgcagg aaatcagcgg
     7081 caccttaagc gccgatacca cgccagcaga agccatctcc cccgttgccg atcaggactg
     7141 gcctgcctat ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc
     7201 tgataacgtc cacaagctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc
     7261 gaacgatccg ggcgaaatca ccaatgaagt gacgccaatt aaagtgggcg acacccttta
     7321 cctgtgtacc gcgcaccaac gtctgtttgc gctggatgcc gccagcggta aagagaaatg
     7381 gcattacgat cctgagctga aaaccaacga gtccttccag catgtaacct gccgtggcgt
     7441 ctcttatcat gaagccaaag cagaaactgc ttcgccggaa gtgatggcgg attgcccgcg
     7501 tcgtatcatt cttccggtca atgatggtcg tctgattgca attaacgctg aaaacggcaa
     7561 gctgtgcgaa accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccggacac
     7621 caaaccgggt ctgtatgagc cgacttcgcc gccgattatc accgataaaa ccatcgtgat
     7681 ggccggttca gtcaccgata acttctcaac ccgtgaaaca tctggcgtga tccgtggttt
     7741 tgatgtcaac accggggagc tgctgtgggc ttttgatccg ggcgcgaaag atccgaatgc
     7801 gatcccgtct gacgaacata cctttacctt taactcacca aactcgtggg caccagcggc
     7861 ctatgacgcg aagctggatc tggtctatct gccgatgggc gtcaccacgc cagatatttg
     7921 gggtggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc
     7981 cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt gggatatgga
     8041 tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccgattat
     8101 ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat cgtcgtaatg gtgaactggt
     8161 ggttccggcc ccggaaaaac cggttcccca aggtgctgcc aaaggcgatt acgtgacccc
     8221 tactcaaccc ttctcggaac tgagcttccg tccgacgaaa gatttgagcg gtgcggatat
     8281 gtggggtgcc accatgtttg accagttggt gtgccgcgtg atgttccacc agatgcgcta
     8341 tgaaggcatt tttacgccgc catctgaaca gggtacgctg gtcttcccgg gtaacctggg
     8401 gatgttcgaa tggggcggga tttccgttga tccgaatcgt gaagtggcga ttgccaaccc
     8461 aatggcactg ccgtttgttt cgaaactgat cccacgcggt ccaggcaacc cgatggaaca
     8521 gccgaaagat gccaaaggca cgggtacgga atccggcatc cagccacagt acggtgtacc
     8581 gtatggtgtc acgctcaacc cgttcctctc accgtttggt ctgccatgta aacagccagc
     8641 atggggctat atctcagcat tggatctgaa aaccaatgaa gtggtgtgga aaaaacgtat
     8701 tggtacgccg caggacagta tgccattccc gatgcctgta ccggtgccgt tcaatatggg
     8761 tatgccgatg cttggcgggc caatctccac ggcgggtaac gtgctgttta tcgccgctac
     8821 ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggtcg
     8881 tttaccagcg ggtggtcagg ctacgccaat gacctatgaa gtgaatggca agcagtatgt
     8941 ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc
     9001 ttatgcgctg ccggatgatg tgaagtaacc ataatgcccg gagagatccg ggcatatttc
     9061 cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc
     9121 agatggcagt gcgccatata agcatgttct ttcggtgcat cgtgattaaa cttcaccagc
     9181 acttcgctga cattaccttc taccttaacg gtatctttcc agcctgcgcg atgcgtcgct
     9241 ggcggtttgc cattttctga caagatacgg aactgcgtac cgtggatatg gaacggatgc
     9301 agcatcatgt cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca
     9361 aacatcggct tattcatatc aaacgcctga ccgttgattt tgttggcatg gtggaaatcg
     9421 aacttcccgc tgtggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg
     9481 ctgtgatcca tcccggccat ggcctgatcg ccatatttct ccatcagcat ctgcatcccc
     9541 atcatatcga gcattgggtc catagaaagt tgcagattgc gtaccgtcag cccttccagc
     9601 gaaggtaacg caggcaggct acttaatgtg tctggcaaag caccagaggc actaatggca
     9661 atcggctgaa tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc
     9721 tggctgaccg gcagcgtcac caggtcaaag ggtttgttat cgttaacctc caccagcact
     9781 tcaaaacgct cgcccatcag cactggcagc tcgttcacct tcaccggttc aggtagcaga
     9841 ccgccgtcgc tggcaatcac ataaagcggg cgattgtcgc tggtggcgaa attgagcgag
     9901 cgggcattac agccattgag caaacgcagg cgcagccagc cacgcggggc agcgtgttgc
     9961 gggtagattg caccgttggt cagcaacgta tcgccaaacc aacccacagc ggcggtcatc
    10021 acatccagtt gataatcaat ctgcccgtct gcgttaaatt ttttatcctg aacaatcacc
    10081 gggacatcat caatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca
    10141 atcaccacca aaccagccag ccccatcgcc acctgacgtc cggtcttgcc atgttgatgt
    10201 ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca
    10261 cctggcggaa taattccctg cgggccgccg tccacttcac ccggtacttc cagcccgtgc
    10321 cagtgcaacg tcgtctcttc cgtcagttgg ttgtagatat caaccgttac cgctttgccg
    10381 cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcggtt
    10441 ttcccgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg
    10501 gtcgtgagca aatcagggat tggtaacgtt gggcgttccg ccgcaaatac tgcgcggctc
    10561 cacagcggca aggctgaagc cacccccagc gcgacggaat atttcaagaa atcacgacgt
    10621 tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc
    10681 ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc
    10741 tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt
    10801 ttttcagaac agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca
    10861 gcgagtctga agccgaagat atggccgatt taacggcagt ttttgttttt ctgaagaacg
    10921 attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcgctggtc tttttcgccc
    10981 agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag
    11041 acagctaccg cgatctcagt ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg
    11101 cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa
    11161 atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgccattt ttttacgggt
    11221 gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg
    11281 accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc
    11341 aggatctgat catttttgag aacgccgcat ttggtcgcgt aatggcgctg gatggcgtag
    11401 tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac
    11461 tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc
    11521 gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg
    11581 tcgtgtcgtt ctgccgtcag tatcttccca accataacgc cggtagttac gacgatccgc
    11641 gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg
    11701 atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagtctg ttcacttcgg
    11761 cattttatga aggctgcaaa cgttgcctga atcctggcgg tattttcgtt gcgcagaacg
    11821 gcgtctgctt tttacagcag gaagaggcca tcgacagtca tcgcaaactc agccattact
    11881 tcagcgatgt tggcttttat caggcggcga tcccgaccta ttacggcggc atcatgacct
    11941 ttgcatgggc gacagataac gacgccttac gccatctttc aaccgaaatt attcaggcgc
    12001 gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcagtccat acggcagctt
    12061 ttgccttacc ccagtatctg caagatgcac tggcttcaca gccgtcctaa ggagaagata
    12121 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
    12181 gtatttacga tatctgctac gccaaaacta ccgaagagcg cgacggttat attgcttata
    12241 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
    12301 tcggggccaa tattcttaac atcgcccgcc aggattacga accacaaggt gccagcgtca
    12361 ctattctggt gagtgaagaa ccggtcgacc cgaaacttat cgacaaaaca gaacaccccg
    12421 gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct
    12481 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
    12541 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
    12601 atattgtgac tattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc
    12661 actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc
    12721 tgtatgacat ggtggatgtg aacgtctatc aggagaatat cttccatacc aagatgttgc
    12781 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
    12841 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
    12901 gcaatatgcc cgctgtttaa cgtctcaggc ggagcacgga ggctccgcct gatttactta
    12961 cttctgctgc acgaaattac ggtaagccgc caccacctga agaaaatcct caacgccaca
    13021 cagtgacaag ctctcttcat cgtagtagtt catcccctct tccatttcat cgccagcgaa
    13081 ttccagttga ttggcgcgaa ccatcacctc ttcaccatcc atccatagcg tgtattcatg
    13141 ccctgcccgt tgccaggaac gttcgctacc tttcaatgcg tgcgccgctt gttccacttc
    13201 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
    13261 ccccatggac atgcgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
    13321 agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat
    13381 cgcagtctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg
    13441 gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattaaac
    13501 cgcagtctgg aaaatcaccc cgtccgcttt ttcggtgtac tgagaaagct ggtcgaagtt
    13561 cagataacgg taagtatcaa cggcagtttt atctacttgc gccacgtagg tctggtactc
    13621 ttccggcgtc ggcagtttgc caatcagcgc cgcaacagca gccagttccg cagaagccag
    13681 gaagacattc gcgccagtac ccaaacggtt cgggaagtta cgggtagagg tggaaaccac
    13741 cgtcgcaccg tctgctacac gcgcctggtt acccatacac agggaacagc cagggatctc
    13801 gatacgagca ccactcttac cgaagacgct gtagtagcct tcttcggtca gctgcgcagc
    13861 gtccatacgc gttggcggtg ccacccacag gcgggtcggc aactgacctt tgtgcgcatc
    13921 cagcagttta ccagcagcac ggaagtgacc gatgttagtc atgcaagaac cgataaatac
    13981 ttcgtcgatc ttctcgccct gtaccgcaga cagcggacgt gcgtcgtccg ggtcattcgg
    14041 tgcacagagg attggctctt tgatatccgc cagatcgatg tcgatcactg ccgcgtattc
    14101 cgcatctgca tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat
    14161 acgacgctcc agggtacgac gatcgccgta accttccgcg atcatccact tcagcaggac
    14221 gatgttagag ttcaggtatt caatgatcgg ttctttgttc agcttgatgg tacaaccggc
    14281 agcagaacgc tcggcagacg catcggtcag ctcaaatgcc tgctcaactt tcagatccgg
    14341 cagaccttca atttccagaa tacggccaga gaagatgttt ttcttacctt tcttctcaac
    14401 ggtcagcaga ccttgtttga ttgcatacag cggaatggcg tgtaccagat cgcgcagggt
    14461 gatacccggc tgcattttgc ctttgaagcg taccagaacg gattccggca tatccagcgg
    14521 cattacgcct gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc
    14581 gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg
    14641 gttcagccag gagtgaatga cgccgtcccc cggacgcagc gacacaccgc cacggttcat
    14701 aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt
    14761 gtggcagaaa gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag
    14821 ttcatcacgg gtcatcgggc cggtggtgtc ctgggaacct acagaagtca ttttcggttc
    14881 acagtacgca cccggacgaa tgcctttcac gccacaggca cggcctacca ttttctgcgc
    14941 cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactatg
    15001 cggcaggcca agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcggaat
    15061 acggccgcca gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggttgccag
    15121 cagttcgccg gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caattacgtc
    15181 gcccatgttc aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt
    15241 gttaaagaag attggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg
    15301 cacatgtgga atatcatcgc ccataaacca cagaacggag ttcgtggcgg atttacgcga
    15361 tgaccccgta cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc
    15421 ttcaatctgt ttgatcgggc caacaacacc aggctgatct ggctcaatac cttcacgggc
    15481 gtttttcagc atcgccagcg cgtgcagtgg gatatccggg cgtgaccacg catccggtgc
    15541 cggagaaagg tcatcggtgt tggtttcgcc ggtcactttg aagacggtaa cggtcagttt
    15601 ttcagccagc gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcattacctg
    15661 cttcgcatat tcgttacctg ctttggcttt ctcttctacg tcatagaagt tatcgaacat
    15721 cagcagtgtg tgagaaagtg ctttggcagc gatcggtgcc agtttggcat catccagcgc
    15781 gtcgatcagc ggatgaatgt tgtaaccacc ctgcatagtg cccagcagtt caatggcttt
    15841 ttccggagtc agcagtgggg atttggcttc acctttggcg acagcagcca ggaagcctgc
    15901 tttgacatag gcggcttcat cgacgccagg gggaacacgg ttggttaaca gatctaacag
    15961 gaattcttct tcgcccgcgg gcgggttttt cagcagctct acaagcgcgg ccatttggtt
    16021 tgcatccagg ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta
    16081 ttcttctagc acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt
    16141 cacgctcctg tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata
    16201 gcaggatttt gacgatgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa
    16261 tcgttaagga tggtctagag attatttccc tccgtaaaaa cttcgttaat tcgcatggtg
    16321 ataatcacct ttctcaacga atcccaagat tagtcaaaat ttaaactacg gcctctttat
    16381 actcggattc acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc
    16441 cgaattatga aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc
    16501 gggctctgtg ccgcctctgc cgggctatat ataaaaagcc gcacagtgga agcgcctgtg
    16561 gaagcgcaat cgacacaaca gactgcgcct gacatctccg cagttacgct tccagcaacg
    16621 gtttccgtgc ctcctgtaac acctgccgtc gtcaaatccg cattcagcac tgctcaaata
    16681 gatcaatggg ttgcacctgt cgcgctatac cccgactctc tgctttcaca agtgttaatg
    16741 gcatcaacct atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt
    16801 aaacaaggcg atgctgctat tcaggcggta tctgaccagc cctgggacgc cagcgtcaaa
    16861 tcactggtgg cctttccaca gttgatggca ttgatgggcg aaaacccgca atgggtgcaa
    16921 aacctgggcg atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg
    16981 cggcaactgg cgcaacaaac cggctcgctg aagtcatcaa ctgaacaaaa gatcatcacg
    17041 acaacgaaaa aagtcgtacc ggtaaatcag cccgccaacg cccctgccac acaatcaaat
    17101 accgtttcta cgtccagccc cgtcgtcgca gaacctgcac cgaccgtaat aaccattgag
    17161 ccagccaatc cggatgtggt ttatattccg aactacaacc caaacgtggt ttacggcagt
    17221 tgggccaata ccgcttatcc accagtttat ctgccgccgc cagccggaga accgtttgtt
    17281 gacagctttg tgcgcggatt cggctatagc atgggcgttg ctaccacata cgcactattc
    17341 agcagcatcg actgggacga cgacgatcat gaccatgacc atcatcatca tgacgatgat
    17401 gattatcatc accacgatgg cggtcatcgt gacggtaatg actggcaaca caacggcgac
    17461 aacatcaata tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct tactgataag
    17521 aatatggcat ggcggcacaa tccaaactac cgtaatggtg tgacctatca tgatcaggat
    17581 atggcaaagc ggtttcatca aaccgatgtc aacggcggaa tgagcgccac gcagttacct
    17641 gctctatcgc gcgacagcca gcgtcaggcg gcagcaagtc agtttcagca acgaacacac
    17701 gccgcccccg tcattacgcg agatacccaa cgtcaggcag cggcacagcg gtttaatgaa
    17761 gcggaacact atggcaatta tgacgacttc cgcgagttca gccgccacca accactgacc
    17821 cagcagcaaa aggacgccgc tcgccagcgt tatcagtctg cctcacctga gcagcgccag
    17881 gcggcccgtg agaaaatgca gactagcccg cagaatcaac agcaaagaga tgcagcacgt
    17941 cagcgtattc agtccgcgtc gcctgagcag cgccaggtgt ttaaggaaaa agtacagcag
    18001 cgcccactga accaagagca acgtgataac gcccgccagc gtgttcaatc ggcatcacct
    18061 gaacaacgtc cgttttttcg ggagaaagtt caggagagcc gcccgcaacg tctaaacgac
    18121 agtaaccgta ctgccaggct gaataacgag cagcggtcag cagtacgcga acgtctctct
    18181 gaacgtgggg caagaagact ggaaaggtaa attgcagacg taaaaaaagc ggcgtggtta
    18241 gccgcttttt taattgccgg atgttccggc aaacgaaaaa ttacttcttc ttcgctttcg
    18301 ggttcggcag gtcggtaatg ctaccttcga acacttctgc cgccaggccc acagactcgt
    18361 gcagagtcgg gtgcgcatgg atggtcagtg cgatgtcttc agcgtcacaa cccatttcga
    18421 ttgccaagcc gatttcaccc agcaactcgc cgccgttagt gccgacaatc gcaccaccga
    18481 tcacacggtg agattctttg tcgaaaatca gcttggtcat accgtctgcg cagtcggaag
    18541 cgatagcacg accagaagca gcccacggga aggtggcggt ttcgtagctg atgcctttct
    18601 ctttcgcttc tttctcagtc agacctaccc atgcaacttc tggttcggta taggcgatgg
    18661 acgggataac tttcggatcg aagtaatgtt ttttaccggc gataacttca gcggcaacgt
    18721 gaccttcgtg aacacctttg tgtgccagca ttggctgacc gacgatatcg ccgatagcaa
    18781 agatgtgcgg tacgttggta cgcagctgtt tgtcaacgcg gatgaaacca cggtcgtcaa
    18841 cttccacgcc agctttgcct gcgtcgaggt ttttaccgtt cggcacacga ccaatcgcta
    18901 ccagcacggc gtcgtaacgc tgcggttcag cgggtgcttt tttgccttcc atcgtcacat
    18961 aaatgccgtc ttctttcgct tcaacggcgg taactttggt ttccagcatc aggttgaatt
    19021 tcttgctgat acgcttggta aagactttaa cgatgtcttt atcagctgcc gggatgacct
    19081 ggtcgaacat ttcaaccacg tcaatctgtg aacccagcgc gtggtatacg gtgcccattt
    19141 ccagaccgat gataccgcca cccattacca gcaggcgttc tggtacttct ttcagttcca
    19201 gcgcgtcagt ggagtcccag atacgcggat cttcatgcgg aataaacggc agttgaatcg
    19261 ggcgagaacc cgctgcaatg atcgcgttgt cgaagttgat cacggtttta ccgttctcac
    19321 cttcaacttc cagggtgtta gccccggtga atttacccag accgttgacc actttgactt
    19381 tgcggccttt cgccatacca gccagaccac cggtcagctg attgatcact ttctctttcc
    19441 aggtacgaat cttgtcgata tcggttttcg gttcgccgaa gacgataccg tgttcagcca
    19501 gcgctttggc ttcttcgata acttttgcta cgtgcagcag tgctttagaa gggatacagc
    19561 cgacgttcag gcaaacaccg ccaagggtgt tgtaacgttc tacgattacg gtttccagac
    19621 ctaaatcagc gcaacggaag gcagcggagt aacctgcggg gcctgcccca agtaccacga
    19681 cctgagtttt gatttcagta ctcatcatga cctctatata tttatctccg gcgggtctga
    19741 cgtttttttt atgacgccca ttatccaccg ggtcgttcta tccgtcggta ttttacaaaa
    19801 ttgttaacaa tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa
    19861 tacattcatg agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca
    19921 ccagacggcg aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag
    19981 caccgtcgat cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga
    20041 actctttacc attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt
    20101 ccggcgcatt cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctcgagatgg
    20161 tgaagcaacc gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa
    20221 tagtcatcag ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg
    20281 ggacaaccag accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg
    20341 tcagacgctg accgtcttcc gacagcgaac tgttgaagcg aggcatctgc tcaagagctg
    20401 cagcaacggc tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg
    20461 ctgcttcttc gttctgctgt ttacggaacg cttccagctc agtgatatcg gttttgtcga
    20521 agtgagtaac gtgcgggatc atcacccagt tacggctcag gttagcacca gagattttct
    20581 gaatgcggcc cagttccact tcttcgattt caccaaactt gctgaagtcc actttaggcc
    20641 acggcagcat gcctgggata ccgccgccag ttgccgccgg agctgcttct gcacgtttga
    20701 ttgcttcttt cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc
    20761 ccttcacttt cgccaggtta acgccaaact cgcgtgccag acggcggatc agcggagtcg
    20821 cgtgaacgta agcgtcgttt tcagcaaatt cagatttgcc ttcggctttc gcagccggtg
    20881 ctgctgccgg ggcttcagct tttgctgccg gagccggcgc tgccgcttcc tgtttcgcag
    20941 gagctgccgc aggcgctgcg ccttcaactt cgaagatcat aatcagcgaa ccagttttca
    21001 ctttatcgcc aacgttgact ttcagttcct tcacgacgcc tgcaaacgga gccggaactt
    21061 ccatagaagc tttatcgcct tctacggtga tcagtgactg ttcagcggca actttgtcgc
    21121 ccactttcac catcacttcg gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa
    21181 cttctttcac gccagctgct ggtgcagagg ccgctgccgg agccgcttcc tgtttagccg
    21241 ccggagctgc cgcgccagct tcacccgcga cttcgaagac cataatcagc gagccggtag
    21301 acactttgtc acccacgttc actttgatct ctttcacggt acctgcaaac ggagccggaa
    21361 cttccataga agccttgtcg ccttctacgg tgatcagcga ctgttcagct tcaactttgt
    21421 cgcccacttt gaccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt
    21481 taacgtcttt tgccgctgca gccgctggtg ctgctgccgg agcggcttct ttcttctctt
    21541 ctgcctgagc aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg
    21601 cgccggtctg ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg
    21661 gagatggtac ttccatagag gctttgtcgc cttctacggt gatcagcgac tgttcggctt
    21721 caactttgtc gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt
    21781 ccggtacttt gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac
    21841 tttatctgca tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc
    21901 gccacgttta gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcaaa
    21961 gtggtgacgc aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg
    22021 gtagtcgtca gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt
    22081 agatgccact gccggagcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc
    22141 cagcgggtgc agcatgttcc agcgttcaca atcctgacca tcacgcgcca gttcggtgaa
    22201 ggaagtcacg ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc
    22261 ttcacggacg tgacgcagga tagaaccgga gcccagcagc tgaactttac ctttgctacc
    22321 ttcaatggtt tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat
    22381 tgccggcatg tggtagtttt cgttcagcgt agtgatgtag tagtaaacgt tctcttgttt
    22441 ttcaccgtac atacgctcca gaccgtcatg catgatgaca gcaacttcgt aagcgtaagc
    22501 cgggtcgtaa gagatacagt tcgggatagt cagcgactga atatggctgt gaccatcttc
    22561 gtgctgcaga ccttcaccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc
    22621 acgcgcttgc tggtcgccag ccgcccagca cagatcgccg atacgctgga aaccgaacat
    22681 cgagtaatag atgtagaacg ggatcatcgg cagattgttg gtgctgtaag aggtcgccgc
    22741 tgccagccag gaacaacctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt
    22801 ctcgtcttct ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt
    22861 cgggctgtaa ataccaatct gacggaacag accttccata ccgaaggtac gcgcttcgtc
    22921 ggcgatgatc ggtaccagac gatctttgat cgacttgttc ttcagcatca cgttcagagc
    22981 acgaacgaaa gcgatagtgg tagagatctc tttgctctgc tcttccagca gcgcgccgaa
    23041 atcttgcagg ctcggcagct ccagcttctc ggtgaagttc ggctggcggc ttggcagata
    23101 accgtgcagt ttctgacgtt gagcgtgcag ataggtatgc tcttcagaac cttccgggaa
    23161 ggtgatgtac ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg
    23221 gatgtgacgc acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc
    23281 agccgcgtcg cccatgccgt aacctttaat ggtatgagca aggattaccg tcgctttgcc
    23341 tttggtttcc tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg
    23401 gttcagtgcc cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata
    23461 tttaccgaag aagtgttcgc gaacatacgc accatctttc gatttgaagg tctggtaatc
    23521 gccgtcaacg gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag
    23581 ttcgtcccaa cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat
    23641 gccttccagt tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag
    23701 gttacagttg ataacgaaga ccagattatc cagtttttca cgggtagcga tggtgatcgc
    23761 acctttggat tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtttgttt
    23821 agaggtatct ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc
    23881 accaatcgga cccagcccca tagatacggt cgggaactgc cagaattccg gcatcagttt
    23941 cgggtgcgga taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg
    24001 ctcctgagtc agacgacctt ccaggaaagc acgagcgtaa acgcctgggg agatgtggcc
    24061 ctggaagtaa accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa
    24121 gcacacatca taaatggttg cggaagactg gaaggacgcc atgtggccgc ccagttcgag
    24181 gtcttttttc gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat
    24241 acggcgttcc agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt
    24301 gatgtagttg ctgataccgg tgcctgcagc tacgttgact ccgcctttgc gggcttcagc
    24361 aagcagttgg tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc
    24421 gatcgcctgg agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga
    24481 catgggttat tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac
    24541 tctcgccgga aagctcaata agacaggttc tacgtttagt tgccgcgctt ttatatgcgc
    24601 ttgatttaca acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct
    24661 ccagacgacg cagagaacgc tcacggcggc tctcttcacg gcttctgtcg agcaaaattt
    24721 cttcgataaa ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca
    24781 taatcgcttc aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg
    24841 aatagagcaa ttcgaagttc tggcggacat tctgagccaa catcggctcc atacacctta
    24901 gcagatgaag cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta
    24961 cggcatttga ttccgcgtcc agatcgccag actgctgcgc cagctctatg gcatggtgga
    25021 gttcacggat gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga
    25081 taccttccag ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga
    25141 gcagctccac cagcggatcg ctgaagcttt gccagaggct gctctggaca aaagtgccgc
    25201 caccctggcg acgaagcaac aagcccttcg cttcgaggcg ttgaatcgcc tcacgcaaag
    25261 agggacggga gacgtcgaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg
    25321 ggcggagagt gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt
    25381 ttggttggcg gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca
    25441 attggtctta ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc
    25501 atacaggttt tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac
    25561 gtttcaaagt tgtaactatg cacaaatgtt aaatttaccc atcaaggggt gaccttaac
//
LOCUS       DACADA010000062        25267 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.063, whole genome shotgun sequence.
ACCESSION   DACADA010000062 DACADA010000000
VERSION     DACADA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.063"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..209)
                     /locus_tag="ISB17_002408"
     rRNA            complement(<1..209)
                     /locus_tag="ISB17_002408"
                     /product="16S ribosomal RNA"
     gene            complement(653..3226)
                     /gene="clpB"
                     /locus_tag="ISB17_002409"
     CDS             complement(653..3226)
                     /gene="clpB"
                     /locus_tag="ISB17_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP1980335.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3356..4087)
                     /gene="pgeF"
                     /locus_tag="ISB17_002410"
     CDS             complement(3356..4087)
                     /gene="pgeF"
                     /locus_tag="ISB17_002410"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAP1980336.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(4084..5064)
                     /gene="rluD"
                     /locus_tag="ISB17_002411"
     CDS             complement(4084..5064)
                     /gene="rluD"
                     /locus_tag="ISB17_002411"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAP1980337.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            5199..5936
                     /gene="bamD"
                     /locus_tag="ISB17_002412"
     CDS             5199..5936
                     /gene="bamD"
                     /locus_tag="ISB17_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAP1980338.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            6206..6547
                     /gene="raiA"
                     /locus_tag="ISB17_002413"
     CDS             6206..6547
                     /gene="raiA"
                     /locus_tag="ISB17_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP1980339.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6651..6698
                     /locus_tag="ISB17_002414"
     CDS             6651..6698
                     /locus_tag="ISB17_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980340.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6797..7957
                     /gene="pheA"
                     /locus_tag="ISB17_002415"
     CDS             6797..7957
                     /gene="pheA"
                     /locus_tag="ISB17_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAP1980341.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELVVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(8000..9121)
                     /gene="tyrA"
                     /locus_tag="ISB17_002416"
     CDS             complement(8000..9121)
                     /gene="tyrA"
                     /locus_tag="ISB17_002416"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAP1980342.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLILSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(9132..10202)
                     /gene="aroF"
                     /locus_tag="ISB17_002417"
     CDS             complement(9132..10202)
                     /gene="aroF"
                     /locus_tag="ISB17_002417"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAP1980343.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10412..10777
                     /locus_tag="ISB17_002418"
     CDS             10412..10777
                     /locus_tag="ISB17_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAP1980344.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            10924..11442
                     /locus_tag="ISB17_002419"
     CDS             10924..11442
                     /locus_tag="ISB17_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAP1980345.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11432..12658
                     /gene="dgcN"
                     /locus_tag="ISB17_002420"
     CDS             11432..12658
                     /gene="dgcN"
                     /locus_tag="ISB17_002420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAP1980346.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12674..13156
                     /locus_tag="ISB17_002421"
     CDS             12674..13156
                     /locus_tag="ISB17_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP1980347.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLITQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(13233..13580)
                     /gene="rplS"
                     /locus_tag="ISB17_002422"
     CDS             complement(13233..13580)
                     /gene="rplS"
                     /locus_tag="ISB17_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP1980348.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13622..14389)
                     /gene="trmD"
                     /locus_tag="ISB17_002423"
     CDS             complement(13622..14389)
                     /gene="trmD"
                     /locus_tag="ISB17_002423"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP1980349.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14420..14968)
                     /gene="rimM"
                     /locus_tag="ISB17_002424"
     CDS             complement(14420..14968)
                     /gene="rimM"
                     /locus_tag="ISB17_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP1980350.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14987..15235)
                     /gene="rpsP"
                     /locus_tag="ISB17_002425"
     CDS             complement(14987..15235)
                     /gene="rpsP"
                     /locus_tag="ISB17_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP1980351.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15372..16733)
                     /gene="ffh"
                     /locus_tag="ISB17_002426"
     CDS             complement(15372..16733)
                     /gene="ffh"
                     /locus_tag="ISB17_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP1980352.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16825..17691
                     /locus_tag="ISB17_002427"
     CDS             16825..17691
                     /locus_tag="ISB17_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAP1980353.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIFQQLIS"
     gene            17712..18998
                     /locus_tag="ISB17_002428"
     CDS             17712..18998
                     /locus_tag="ISB17_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP1980354.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(19052..19645)
                     /gene="grpE"
                     /locus_tag="ISB17_002429"
     CDS             complement(19052..19645)
                     /gene="grpE"
                     /locus_tag="ISB17_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP1980355.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19768..20646
                     /gene="nadK"
                     /locus_tag="ISB17_002430"
     CDS             19768..20646
                     /gene="nadK"
                     /locus_tag="ISB17_002430"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAP1980356.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20732..22393
                     /gene="recN"
                     /locus_tag="ISB17_002431"
     CDS             20732..22393
                     /gene="recN"
                     /locus_tag="ISB17_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP1980357.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22542..22883
                     /gene="bamE"
                     /locus_tag="ISB17_002432"
     CDS             22542..22883
                     /gene="bamE"
                     /locus_tag="ISB17_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAP1980358.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22945..23235)
                     /locus_tag="ISB17_002433"
     CDS             complement(22945..23235)
                     /locus_tag="ISB17_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAP1980359.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(23225..23701)
                     /gene="ratA"
                     /locus_tag="ISB17_002434"
     CDS             complement(23225..23701)
                     /gene="ratA"
                     /locus_tag="ISB17_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAP1980360.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGG
                     WKFTPLSQDACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23833..24315
                     /gene="smpB"
                     /locus_tag="ISB17_002435"
     CDS             23833..24315
                     /gene="smpB"
                     /locus_tag="ISB17_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP1980361.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24530..24892
                     /gene="ssrA"
                     /locus_tag="ISB17_002436"
     tmRNA           24530..24892
                     /gene="ssrA"
                     /locus_tag="ISB17_002436"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6434 a         6641 c         5894 g         6298 t
ORIGIN      
        1 aaggtccccc tctttggtct tgcgacgtta tgcggtatta gctaccgttt ccagtagtta
       61 tccccctcca tcaggcagtt tcccagacat tactcacccg tccgccactc gtcagcgaaa
      121 cagcaagctg tttcctgtta ccgttcgact tgcatgtgtt aggcctgccg ccagcgttca
      181 atctgagcca tgatcaaact cttcaattta aaagtttgat gctcaaagaa ttaaacttcg
      241 taatgaatta cgtgttcact cttgagactt ggtattcatt tttcgtcttg cgacgttaag
      301 aatccgtatc ttcgagtgcc cacacagatt gtctgataaa ttgttaaaga gcagtgccgc
      361 ttcgcttttt ctcagcggcg cggggtgtgc ataatacgct ttcccgctac agagtcaagc
      421 atttattttt gctttctctg ccggaattct caggagaacc ccgctcaccc ggcggcgtgt
      481 ttgccgttgt tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagcg
      541 aaaaatataa aaactttatc gttcgctcat ttttcaggca aaacatctta aatatagtct
      601 tttccgtcta acttatagac aaaaacgagc cccgaagggc tcgttttatc atttactgga
      661 cggcgacaat ccggtcttca ttaacttcca ggcgaatcac tttacccgga accaattcac
      721 cagacagtat ttgctgtgcc agcgggtttt caatctgctg ctgaattgca cgtttcagag
      781 gacgtgcgcc atagaccgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      841 aaatgtggat ttcataacca cgttcttcca gacgtttata cagacgtttc aactgaatct
      901 gcgcaatcga cgcaatgtgc tgttcaccca gcggatggaa gaccaccact tcatcgatac
      961 ggttaatgaa ttccggacgg aagttatggc taaccacacc gagcaccagc tctttcatgt
     1021 gcgcataatc cagttcaccg aagcgttcct gaatcaaatc ggaaccgagg ttagaggtca
     1081 taatgacaac cgtattacgg aagtcgaccg ttctcccctg cccgtcagtc agacgcccat
     1141 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
     1201 gcagaatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
     1261 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
     1321 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gctagcgcct
     1381 tacaaagctc agttttcccc acaccggttg ggccgaggaa caggaatgaa ccaatcgggc
     1441 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
     1501 cttcgttctg accaataacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1561 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1621 tttcggcatc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1681 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1741 ccaggtcacc cacacggcga gcctgttcaa tagcaatttt cgcctgttcc agttccgctt
     1801 taatggtctg cgtaccagaa agcgaggctt tctctgcttt ccactcttct tctaactcgg
     1861 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccaggcgt tttttactgg
     1921 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1981 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     2041 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     2101 acgtcgctgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc agctcgtaac
     2161 gttctttcag gcctcgcaga atagcaatgg tatcttcaac agaaggctcg gcaacaaaca
     2221 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     2281 gcgtcgtggc acctacgcag tgcaattcac cacgcgccaa cgctggtttc agcatgtttc
     2341 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     2401 acaggatgac gttgccttcc tgtttagcaa gatcgttaag tacgcctttt aaacgttctt
     2461 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtacgc
     2521 ggcggccttt caacccttcg ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2581 cgatggcagt tttaccgacg ccgggttcac caatcaatac cgggttattt ttagtacgac
     2641 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2701 tgccttgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2761 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2821 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2881 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2941 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     3001 cttcaacctg tggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     3061 taatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     3121 tcattaaatg aagtggttcg atgaattggt tgtcgtgccc gagtgcaagt gattgggcat
     3181 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     3241 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     3301 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     3361 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     3421 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgttcaaca ccaacgctcg
     3481 ccagacgctg ccgggcaagc tgataaatat ccgccagata cttatcacca tgctgaatga
     3541 aagctgtact cgctttggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3601 cgaacgcacg tggcccaatt gccggcccta accaggcgag aatattttcc ggattatcag
     3661 caaaacagga aaccgtctct tccaacacgc ctgcgcacag tccacgccag ccagcatgag
     3721 cggcggcgac ctccgttccc gcacgattgc aaaacagcac gggcaggcag tcggcagtca
     3781 tcactgcgca aaccgtgcca ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3841 aaggttcgcc aataagctta agcacgtctt tgccgtgtac ctgctcaagc cagaccggtt
     3901 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctccccaacg tgatccgggt
     3961 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggtaagctc acaccgccga
     4021 tacgggtaga gctacaagcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     4081 tactcataac cagtccactt catccttatg ctcttcgaaa tcggcgcgca tctcctcaat
     4141 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatgc cggagatcgg
     4201 gtgataaaga cgcagcatgg ttgcatgaag cgcctgacgg tcaaacttac gcagcgtgga
     4261 gataaacgct tccgaagcac cttttggcgg acgcggacgt ccaccataaa ccggatcgcc
     4321 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccggtttc
     4381 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtgac
     4441 cgccggttta cccatgggat gtaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     4501 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4561 tgtaatttca cgccgttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4621 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acaatgcccg cacgcggtac
     4681 atcggcaatg ggcggataat aatgaagcaa tgcattcagt accgtgccat ccgggttacc
     4741 cgcgccagga tgtaccacca ggtcgcgcgg tttattaata acgatgatat cttcatcttc
     4801 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4861 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac acactttgcc
     4921 gttaaccagc acacgctggt cgaggatcca ttcttttatc cgcgaacgtg aataatccgg
     4981 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     5041 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     5101 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     5161 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     5221 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     5281 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     5341 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     5401 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     5461 cacaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     5521 tcatgtacat gcgtggcctg accaatatgg cgctcgatga cagtgcactg caagggttct
     5581 ttggcgtcga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5641 ccaaactggt gcgcggctat ccgaacagtc agtacaccac cgatgccacc aaacgtctgg
     5701 tattcctgaa agatcgtctg gcgaaatatg aatactccgt tgccgagtat tatacagaac
     5761 gtggtgcatg ggttgctgtc gttaaccgcg tagaaggcat gttgcgcgac tatccggata
     5821 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5881 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5941 aacctgaaac acaaaacggc agccttgagc tgccgttttt ttattctgtt agttgtgaaa
     6001 ctgaagcgat ttagtcacta tcgatctcat caaatatggc ccgctttgag atattcctca
     6061 agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac aaaaagtgac
     6121 aaaattatga gatttccatc acacattttg acatcaggaa cgttatgctg aattcaccaa
     6181 gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac aaatggaaat
     6241 tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac
     6301 acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg ttgctgacgc
     6361 cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag atatgtacac
     6421 cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc agcacaaagg
     6481 cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga
     6541 agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac
     6601 agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac atgaaacaca
     6661 caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg
     6721 aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata
     6781 acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag aaaaaatcag
     6841 cgcgctggat gaaaaattat tagcattact ggcagagcga cgcgaactgg tcgtcgaggt
     6901 gggaaaagcc aaactgcttt cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt
     6961 gctggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg
     7021 cctgttccag ctcatcattg aagattccgt attaactcag caggctttac tccagcaaca
     7081 tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca aaggttctta
     7141 ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg
     7201 ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt
     7261 cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc tgctgcaaca
     7321 taccagtttg tcgattgttg gcgagatgac gttaactatc gatcattgtt tgttggtctc
     7381 cggcactact gatttatcca ccatcaatac ggtctacagc catccgcagc cgttccagca
     7441 atgcagcaaa ttccttaatc gttatccgca ctggaagatt gaatataccg aaagtacgtc
     7501 tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga
     7561 agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga atcagcgaca
     7621 aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtttctg accaggttcc
     7681 ggcgaaaacg acgttgttaa tggcgaccgg acaacaagct ggtgcactgg ttgaagcgtt
     7741 gctggtactg cgcaaccaca gtctaattat gacccgtctg gaatcacgtc cgattcacgg
     7801 taatccgtgg gaagagatgt tttatctgga tattcaggcc aatcttgaat cagcggaaat
     7861 gcaaaaagca ttgaaagagt taggggaaat cacccgttcg atgaaggtat tgggctgtta
     7921 cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaagtgccg gatgatgtga
     7981 atcatccggc actggattat tactggcgat tgtcattcgc ctgacgcaat aacacgcggc
     8041 tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt
     8101 caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat
     8161 agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg gcataaagct
     8221 gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa atcggcgaag
     8281 agagcgccag tagttgctca agctgaacat tttcttctgc caggtgcagc ccataagcaa
     8341 aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc
     8401 taatacgatg caaccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt
     8461 ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccgggc
     8521 cgaacatcgg gtgtaacccc agcaccgggc catcgtgcgc cgccagcatg gcctgtaatg
     8581 gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa ggcggtaatt
     8641 tgccgataac ttgctcagta acgtggatgg gcacactaac aatcaccatt ccggcatcgg
     8701 caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg
     8761 agagtatcag catcttctcg aacagacgtc ccatctgacc gccaccaccg acgataacca
     8821 ccgggcgcag tgacggacaa agcgttttaa atcctttgtc gttttcactg gagtaagatt
     8881 cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg
     8941 cctccgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc
     9001 caaatcggct tttcacctcg cccacttcag caaccaattc cagacgcttc gctaataaat
     9061 tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaac tcagcaacca
     9121 taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca
     9181 cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat
     9241 ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt actttcgatc
     9301 atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc
     9361 gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac
     9421 gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag
     9481 tttggcgctt taccaccgcg caggatcaca tgcccgtccg gattcccctg agtttgtagc
     9541 aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc
     9601 atagcgttaa ttgccgttgc cagactgcca tcggtgccgt ttttaaaacc aaccggcatg
     9661 gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaattgct
     9721 gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc
     9781 agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct
     9841 tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg
     9901 gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg
     9961 gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa
    10021 ggaccacata ctaccagaag acgaggatcg cgcccggcga taatatctga aatggtttta
    10081 cgcgagtcag caatctgggc ttcttgttgc aggcttaatg gaaaagcggc cttcagttgt
    10141 tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttttgc
    10201 atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa
    10261 cgataacaca taaagtaaag ttttcaatcc atatttcgta cacatttctt tacgtaagca
    10321 atttattcgc gctttttacc cttatttctg tatatataaa tttacaccat ctttaaaaac
    10381 aatccgctat gctttgaaaa aggagaaaga aatgatgaaa aaatttatcg cccccttgtt
    10441 ggctttactg gttagcggat gtcagattga tccttatact cacgcgccaa ccttgaccag
    10501 caccgactgg tatgatgttg gtatggaaga tgcgatatcg ggcagcgcca ttaaagatga
    10561 cgatgcattt agcgattcac aggcggatcg cggtctatac cttaaaggat atgccgaagg
    10621 gcaaaagaaa acttgccaga ccgattttac ttatgcccgg gggctttctg gtaaaagctt
    10681 tcctgccagc tgcaataatg ttgaaaatgc cagccaactg catgaagtat ggcgaaaagg
    10741 ggctgatgaa aatgccagca caatacgatt aaattaatta cccccaaaat aaattaagca
    10801 tgagatttta ctcattatgt tttatttctc acattgatga cgaccgcatg aaaggatgat
    10861 attttaatga gcattctgaa aaaatatcga tgcatttcga gcgaagatgg tgaggatccc
    10921 tgaatgcgtt tttctcaccg actgttctta ctgctgattc tcctgctgac gggagcacca
    10981 atactcgctc aggaaccttc tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt
    11041 gtcagttaca cccgctggcc tgggctatca gggccaccaa agttatgtat cttttcatct
    11101 tcacgtttta gtaccgcctt acaagaaaat gctgctacat ctttacctta tctgcctgtc
    11161 atcattcata ctcaacaaga ggcgataatt tcaggctgca atggttttta ttttggcaac
    11221 gagtcaccga cattccagat ggaattaacc gaacaatatc cgtcaaaggc gttattatta
    11281 attgccgaac aaaataccga gtgcattatt ggtagcgcct tttgtctgat catccacaat
    11341 aatgacgtca gatttgccgt aaacctggat gccttatcgc gtagcggtgt aaaggtcaac
    11401 ccggatgtct taatgctcgc acggaagaaa aatgatggat aacgataatt ctcttcataa
    11461 gcgccccacg tttaaaagag cattacgcaa catcagcatg accagcatat ttatcactat
    11521 gacgctgatc tggctgctgc tttccgtgac gtcagtgcta accctgaaac aatacgcgca
    11581 aaaaaacctg gcactgacag cagcaacaat gacttacagt ctggaagcag ctgtcgtttt
    11641 tgccgatggc cctgcagcaa ctgaaacact ggcagcgctg ggccagcaag gccaattttc
    11701 aactgcagaa gtgcgtgata agcagcaaaa tattctggca tcctggcatt acacccataa
    11761 ggaaccaggc gatactttca gcaatttcat aagccactgg ctcttccctg cccccatcat
    11821 tcagccgatt cgtcacaata gtgaaaccat tggcgaagta cgcttaactg ctcgcgacag
    11881 ttcaatcagc cattttatct ggttttcgct cgctgtcctg accggttgta ttctgctggc
    11941 atcaggcatc gcaattaccc tcacccgcca tttgcacaat ggcctggtgg aagcgctgaa
    12001 aaatatcacc gatgtcgtac atgatgtgcg ttctaaccgc aatttttccc gacgagtttc
    12061 ggaagaacgt atcgctgagt ttcaccgctt cgctctcgac ttcaacagtc tgctggatga
    12121 aatggaagag tggcagcttc gtttacaggc caaaaatgcg cagcttctac gtaccgcgct
    12181 acatgaccca ttaaccgggc tggctaaccg cgcagcgttt cgtagcggca tcaacacgtt
    12241 gatgaacaat tccgatgccc gaaaaacgtc ggcgttacta tttcttgatg gcgataattt
    12301 caaatatatc aatgatacct ggggtcatgc gacgggcgat agagtcttga ttgaaatagc
    12361 aaaacggtta gctgaatttg gcgggctgcg acataaagca taccgcctgg gcggcgatga
    12421 attcgctatg gtgctctatg atgtacagtc ggaatctgaa gtgcagcaga tatgctcagc
    12481 actgacacaa atttttaatc tcccgtttga tcttcataat ggccatcaaa ctaccatgac
    12541 attaagcatt ggttacgcga tgaccattga gcacgcctct gcggaaaatt tacaagagct
    12601 tgccgatcac aatatgtatc aggccaaaca ccagcgtgcc gaaaaactgg tgagataaca
    12661 aggatatatc gatatgataa agcacctgct agcccccctg attttcacct cgctaatact
    12721 tactggctgc cagtcccctc agggaaagtt tactcctgag caagtcgccg ctatgcaatc
    12781 ttatggattt actgaatccg ccggcgactg gtcgctgggc ttatcagatg ccattctgtt
    12841 cgcaaaaaat gactacaaat tgctcccgga aagccagcaa caaatccaaa ccatggcagc
    12901 caaattggcc tcgacagggc taacacatgc ccgtatggat ggacacaccg acaactatgg
    12961 tgaagacagt tacaacgaag tcttatcatt aaaacgggcg aatgtcgtgg ccgatgcatg
    13021 ggctatgggt ggacaaattc cgcgcagcaa tctcatcaca cagggtttag gaaaaaaata
    13081 tcccatagcc agtaacaaga ccgcccaggg ccgcgccgaa aaccgccgcg tcgcagtggt
    13141 gattacaacc ccttaaaaca cgagcaaaca gacaaaaaaa gggccagcca attggccagc
    13201 ccttcttaac aggatgtcgc ttaagcgaaa tcttagttaa gacgctcttt gatacgagca
    13261 gccttaccag tacgctcacg caggtagtac agtttagctt tacgaacagc accacgacgt
    13321 ttgacagaaa tgctgtcaac taccggagag tgagtctgga agacacgctc aacgccttcg
    13381 ccgttggaaa ttttacgaac agtgaatgca gagtgcagac cgcggttacg aattgcgata
    13441 accacgccct cgaatgcctg cagacgtttt ttggaacctt caacaaccca tactttcact
    13501 tccacggtat cacccggacg gaaggaaggt acgtcctgct tcatctgctc ttgttcaagt
    13561 tgcttaataa tgttgctcat aatttaatct cttatcctgg gtaaactgat atctcggggg
    13621 cttacgccat cccatcatgt ttatgttgct gttgtgcgtg ttccgttttg aactccgcca
    13681 gcaaccttgc ttgctcttca gtcagagcca ggttttccag aagttcaggt cttctaagcc
    13741 aggtacggcc cagcgactgt ttcaaacgcc agcgacgtat ctcggcatgg ttgcccgaca
    13801 gtaacactgg cggaacttcc atcccttcta acacctcagg ccgcgtatag tgcgggcaat
    13861 ccagcaatcc ttcagcaaag gagtcttccg ttgccgaggc ctcatgtccc agtactcccg
    13921 gaataaaccg ggaaacggag tcaatcagcg tcattgctgg taactcacca ccactgagaa
    13981 cgtaatcgcc gattgaccat tcttcatcaa tttcggtttg gatcacgcgc tcatctatac
    14041 cttcgtagcg accgcacacc agaatcaatt tttgattcgt tgccagctcg ctgacgcccg
    14101 cttgatcaag cttgcgtccc tgtggtgaca gataaatcac ctttgcgcct tcacccgccg
    14161 cggcttttgc tgcatgaatg gcgtcccgca agggttgcac catcattaac atccccggtc
    14221 cgccgccgta aggacgatcg tccacggtac ggtgccgatc atacgtgaag tcgcgaggac
    14281 tccagctctg gatgctcagc aggccatttt taactgcccg gccagttacc ccgtaatcgg
    14341 taattgcgcg gaacatttca ggaaacaggc taattatgcc aatccacata gcgccgtctt
    14401 ttaccgttta tccggtggtt taaaaaccag gatcccaatc tacttcgatt gaacgagtag
    14461 tgagatcgac tttcttgata acctgcccat cgaggaacgg tacgagacgt tccttgatac
    14521 caaacgcatc tttcaggttt gccttaatga cgagaacgtc attagatccg gtttccatca
    14581 tatctacgac tttaccgaga tcgtagcctt cagtggttac tacctggcag cccatcaggt
    14641 ctttccagta gtagtcaccc tcttcaagct gaggcaactg cgatgaatcc acgacaattt
    14701 cacaattcgt cagcaggttc gccgcatcac gatcgtcaac gcctttcagc ttgatgatca
    14761 tgtcctgatt gtggtgcttc cagctttcca gctggacttg ctgccactga cccgccttct
    14821 ggataaacca gggctgatag tcaaaaatgc tttcggcgtc ttcggtggaa gaaaacactc
    14881 tgagccaccc acgaataccg taagacgaac ccatttttcc caaaacgatg ggatcaacag
    14941 gtgcttgcgc ggtgagttgt ttgctcatca tgaccaccgt gacagattaa gctgctttgt
    15001 ttacttcttt gatcagcgca gcaacgcgat cagaaatagt tgcgccctgg ccaacccagt
    15061 gagcgatgcg atccagatcc aggcgagtgc cttcttcttt ttcgctagcg attgggttga
    15121 agaaaccaac gcgctcgatg aagcgaccgt tgcgtgcatt acggctgtca gcgacaacaa
    15181 cctggtagaa cggacgcttt ttagcgccgt gacgtgctaa acgaatagtt accataacat
    15241 cctcttgtgt gaataaaaca accggacccc atcgaggaac ggagtccggt gtcatattaa
    15301 aagcccgaaa attttactga tttctggaga aaatgcaatc agcagttaat aactctgtcg
    15361 cataaggtca tttagcgacc aggaaatccc ggcggcatca tccctttcat gcttctcatc
    15421 atcttcgcca tcccgccctt cttcattttc ttcatcatgc gctgcatgtc gtcgaactgt
    15481 ttcagaagac ggttaacgtc ctgcacctgc ataccgcaac cggcagcgat acggcgctta
    15541 cgcgaacctt tgatgatttc tggcttagcg cgctctttca tcgtcatcga gttgatgatg
    15601 gcttccatac gcaccagcac tttatcgtcc atctgcgact tgacgttatc cgggatctgc
    15661 cccatgcccg gcagcttgcc catcaggcta gccatgccgc ccatattttt catctggcgc
    15721 agctgctcaa gaaagtcgtt gagatcgaag ccgtcgcctt ttttcagctt gctggctaat
    15781 ttctcagctt gcgcgcggtc aactttgctt tcgatatctt cgatcagtga cagtacgtcg
    15841 cccatgccga gaatacggga ggcaatacgg tccggatgga atggctccag cgcctcggtt
    15901 ttctcgccaa caccgaggaa cttgatcggt ttgccagtga tgtgacgaat agagagcgcc
    15961 gcaccgccgc gggcatcgcc gtccactttg gtcaacacta caccggtaag cggtaacgct
    16021 tcattgaatg cttttgccgt attggccgca tcctgaccgg tcatggcatc gaccacaaat
    16081 agggtttcaa ccggattaat cgacgcatgg acttgtttga tctcgtccat catcgcttcg
    16141 tcaacgtgca gacgaccggc agtatccacc agcagcacgt cgtagaattt cagtttggct
    16201 tctttcagcg ccgcgttaac gatatctacc ggcttctgac caacatcaga agggaagaaa
    16261 tcaacgccca cctgctctgc cagcgtctca agctgtttga ttgccgccgg gcgatagacg
    16321 tcggcagaaa ccaccagtac tttcttcttg tgcttctcgc gcaggaactt accgagttta
    16381 ccaacgctgg ttgttttacc ggcaccttgc aggcccgcca tcagcacgac cgcaggcggt
    16441 tgcgcagcca ggttcagggt ctggttctct tcgcccatcg ccgcaaccag ttcgttacgg
    16501 acaattttga cgaactcctg ccccggcgtc aggctcttat taacttcatg accaaccgct
    16561 ttctctttta cgcgattgat aaactcacgc actaccggca gagcgacgtc agcctccagc
    16621 agcgccatgc gcacttcgcg cagcgtatct tttacgttgt cttcagtgag gcgtccacgg
    16681 ccactgatat tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa cattgtctct
    16741 cgcctggggt ggaaacggtt ggccgcaatc gcgacacatc atcagtattt tgccgcagta
    16801 taacatgaag gcgtctttgt tgttatgcaa cggttggagc agcgttcacc tgacgctata
    16861 ctgcttctct ttcttattgc tcaaactgtc gacattacta tgcccgtttt tgctctgctc
    16921 gcgcttgtcg cctactccgt cagtcttgcg ctgattgttc ccggtctgct gcaaaaaaac
    16981 ggcggctggc ggcgcatggc tattatttct gcggtcatcg cgctggtctg ccacgcaatc
    17041 gctctggaag cccgcatcct gcccgacggt gatagcggac aaaacctcag cctgctgaac
    17101 gttggttcat tggtcagttt gatgatctgt acggtaatga ccattgtggc ttctcgcaat
    17161 cgtggctggt tgttgctacc cattgtctat gcctttgcgc ttatcaacct agcgctggcg
    17221 acctttatgc ccaatgaata catcacccat ctggaagcaa cgccggggat gctggtgcac
    17281 attggcttat cgcttttttc ctatgccacg ctaattatcg ctgccctgta cgcgctgcaa
    17341 ctggcgtgga ttgattacca actgaagaac aagaagctgg cgtttaacca ggaaatgccg
    17401 ccattgatga gtatcgagcg taaaatgttc catatcacgc agattggcgt ggtgctgcta
    17461 acgctcactc tttgcactgg cctgttctac atgcacaact tatttagcat ggaaaatatc
    17521 gacaaggccg tgctctctat cgtggcgtgg tttgtctata ttgtgctgct gtggggacat
    17581 tatcatgaag gatggcgtgg acgccgcgtc gtctggttta acgttgcggg tgcagtcatt
    17641 ctgacactgg cctacttcgg cagccgaatt tttcagcagt taatcagcta aacccagaaa
    17701 aggagtttcc cctggaacac atttctacta ctacgttgat cattattctg atcatcatgg
    17761 tggtcatttc agcctatttt tccgggtccg aaaccggaat gatgaccctc aaccgctatc
    17821 gtctgcggca tatggcgaaa cagggtaatc gctcggccaa acgcgtcgaa aaattgctgc
    17881 gtaagccaga ccgcctgata agcctggtgt taatcggcaa taacctggtc aatattcttg
    17941 cctccgcgct cggcactatt gttggaatgc gtttgtacgg cgatgcgggc gtggcaattg
    18001 cgactggtgt gctgactttt gtcgtgctgg tttttgctga ggtattgcca aaaaccattg
    18061 ccgcgctgta cccggaaaaa gtcgcttatc cgagtagttt tctgctggct ccgctgcaaa
    18121 ttttgatgat gccgctggtc tggttgctga atgctattac ccgcatgtta atgcgcatga
    18181 tgggcatcaa aaccgatatc gtggttagtg gctctttaag caaagaagaa ttacgcacca
    18241 ttgtgcacga atcgcggtca caaatttccc gtcgcaatca ggatatgctg ctgtcggtac
    18301 tcgatctgga aaagatgacc gtcgatgaca tcatggtgcc gcgcagtgaa attatcggta
    18361 ttgatatcaa cgatgactgg aaatcgattc tgcgccaact ctcccactca cctcacgggc
    18421 gcatcgtgct ctaccgtgat tcgctggacg acgccatcag tatgctgcgt gtgcgtgaag
    18481 cctggcgact catgtcggag aaaaaagagt tcaccaaaga aaccatgcta cgcgccgcgg
    18541 acgagatcta ttttgtcccg gagggtacgc cgctcagcac acagttggtg aagtttcagc
    18601 gcaacaaaaa gaaagtcggc ctggtcgtca acgagtatgg agacattcag gggctggtga
    18661 cggtcgaaga tattctcgaa gagattgtcg gcgatttcac cacgtcgatg tcgccaacac
    18721 ttgccgaaga ggtcacgccg caaaacgacg ggtcggtgat tatcgatggc accgccaacg
    18781 tgcgagaaat taacaaagcc tttaactggc atctaccgga agatgatgct cgtactgtta
    18841 acggcgtcat tctggaagcg ctggaggaga ttccggtagc aggcacccgc gtgcgtatag
    18901 gcgagtacga tatcgatatt ctcgacgtac aggacaatat gattaagcag gtaaaagttt
    18961 ttcctgtaaa accgctgcgc gagagtgtgg cggagtaaaa aaacggcccg gcattcgcat
    19021 gctggaccgt gatttattac gaaaacagaa attaagcttt tgccttcgct acagtcacca
    19081 tcgccgcacg aattgtacga ccgttcagcg tataaccctt ctgcataatg cccagtacgt
    19141 tacctggcgc aacgtcatca gattccacca ttgcgatggc ctgatgcaca ttcgggtcca
    19201 gtggtacgtt agtttcggcg atcacttcaa cgccaaactt acgcacaaca tccagcatcg
    19261 acttcagcgt cagctcaata ccttcaacca tcgcggacat atccgggtta gctttatcag
    19321 ccacttccag cgcacgatcc aggctatcaa tcaccggcag caattcgttg atgaatttct
    19381 ccagcgcgaa tttgtgagct ttttcaatat ccagttcagt acgacgacgc aggttttcca
    19441 tttcggcttt tacgcgcaaa atgccgtcac gttcacgggt ctgggcttca gccagctgag
    19501 cttcgagatt cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa gcttctggct
    19561 caactgcctc aatctcttcg tgctgatcca tgataatttc ttccggggct tgcccctcag
    19621 gcgttttctg ttctttacta ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct
    19681 aacttcgctt attatgggga tcagtttcag ggtttcaagg gaagcactca cattgtcatc
    19741 aatcttcgca acaaggacct cggaaaaatg aataatcatt tcaagtgtat tggcattgtg
    19801 ggacacccac ggcaccccac tgcactgaca acacatgaaa tgctctaccg ctggctgtgc
    19861 acaaaaggtt acgaggtcat cgttgagcaa caaatcgctc acgaactgca actgaagaat
    19921 gtgaaaactg gcacgctcgc ggagattggg caacaggctg atctcgcggt tgtcgttggc
    19981 ggcgacggta atatgctggg cgcggcgcgc acgctcgccc gctacgatat taaagttatt
    20041 ggaatcaacc gtggcaacct gggtttcctg actgaccttg atcccgataa cgcccagcaa
    20101 caattagccg atgtgctgga aggccactac atcagcgaga aacgtttttt gctggaagcg
    20161 caagtctgtc agcaagattg ccagaaacgc atcagcacgg cgattaacga agtggtactt
    20221 catcctggca aagtagcgca tatgattgag ttcgaagtgt atatcgacga gatctttgcg
    20281 ttttcgcagc gttccgatgg actgattatt tcgacgccaa caggctccac cgcatattcc
    20341 ctctctgcgg gcggtcctat tctgacaccc tctctggatg cgattaccct ggtgcccatg
    20401 ttcccgcata cgttgtcagc acgaccactg gtcataaaca gcagcagcac gatccgtctg
    20461 cgtttttcgc atcgccgtaa cgacctggaa atcagttgcg acagccagat agcacttccg
    20521 attcaggaag gtgaagatgt cctgattcgt cgctgtgatt accatctgaa tctgattcat
    20581 ccgaaagatt acagttattt caatacatta agcaccaagc tcggctggtc aaaaaaatta
    20641 ttctaatttt acgccagcct ctttactgta tataaaacca gtttatactg tacacaataa
    20701 cagtaatggt ttttcataca ggaaaacaac tatgttggca caactgacca tcagcaactt
    20761 tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga
    20821 gaccggcgcg ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc
    20881 tgaagccgac atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct
    20941 gaaagatacg ccagcggccc tgcgttggct ggaagaaaac cagcttgaag acgggcatga
    21001 atgtttgctt cgtcgcgtaa tcagcagcga tggtcgctcc cgtggtttca tcaacggtac
    21061 agctgttcct ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca
    21121 gcacgctcat cagttactca ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc
    21181 caatgaaacc tctcagctcc aggaaatgac cgcacgttat cagttgtggc atcaaagctg
    21241 ccgtgacctc gcgcatcatc agcagttaag tcaggaacgc gccgcccgtg cggagctgct
    21301 gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cctggagagt ttgagcaaat
    21361 cgacgaagag tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc
    21421 attggcatta atggctgacg gtgaagacgc aaatctgcaa agtcaacttt acacggctaa
    21481 acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct
    21541 ggaagaggct accatccaga ttgctgaagc cagtgatgaa ctacgccact actgcgatcg
    21601 tctggatctc gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc
    21661 gctggcacgt aaacatcacg tcagccctga ggcattgcca cagtattacc agtcgctact
    21721 ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt
    21781 aacgaaacat catcagcagg cactggaaac ggcgcgcgcg ttacaccagc aacgccagca
    21841 ttatgcaaat gagttggcac agctgatcac cgatagtatg catgcactct ctatgccaca
    21901 tgggcagttt acgatcgatg ttaaatttga cgagcatcac ctgggcgctg acggcgctga
    21961 tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt
    22021 cgcatccggt ggtgagttgt cccgcatcgc attggcaatc caggtcatta ccgcgcgtaa
    22081 aatggaaacc ccagcactga tttttgatga agtggatgta gggattagcg gcccaacagc
    22141 ggcagttgtc ggcaaactgc tacgtcaact tggcgaatca actcaggtga tgtgtgttac
    22201 ccacctgcca caagtcgcgg gatatggtca tcaacactat tttgtcagca aagaaaccga
    22261 tggtgcgatg acagaaacgc atatgcaatc cctggataaa aaagcgcggt tacaagagtt
    22321 ggcgcgcctg cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact
    22381 gcttgcggcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag
    22441 accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat
    22501 gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga
    22561 ctgctgcagc agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg
    22621 tttaccgtcc tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac
    22681 gtgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc
    22741 catttggtac aaatacctgg ttctatgtct tccgccagca acctggtcat gaaggtgtaa
    22801 cgcagcaaac gctgacgctg acctttaaca gtagcggtgt gttgaccaac attgataaca
    22861 aacctgcgct gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct
    22921 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
    22981 aagctctttc ggatcggcaa tgagaggacg ataaatctcc acccgatcgc catcatgcac
    23041 aatatcgctg agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
    23101 atcggtacgc aattccagca aaccactggc gcgaatagct tcttcaaccg tcgcgccctc
    23161 ctgcaacgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
    23221 aattttacct ggcactgtag acctcttttg cacgaacagt aaaagcctgc accatattag
    23281 ctgccagctc tttaaacaca cgaccaaagg cgagttcaat caacttattg gtaaactcaa
    23341 agtcgagatg aaactcaata cggcacgcgt cctggctcaa cggcgtaaac ttccaaccgc
    23401 ctatcaattt cttaaacggt ccatcaacca gactcatgag aatactttga ttactggtca
    23461 actggttgcg ggttgtaaac gttttgctga tcccagcctt agacacatct accgcagcgg
    23521 tcatctgccc aggagtggac tccagaatcc ggcttccagt gcaccctggt aaaaactgag
    23581 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
    23641 cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca
    23701 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcggga tcatgctaag
    23761 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
    23821 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
    23881 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
    23941 caaggctggg aggttaaatc cctgcgtgca ggaaaagcca atatcagcga cagctacgtc
    24001 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggctgtggcc
    24061 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
    24121 ctggactcat tgtacggtcg cgtaaatcga gaaggctata ccgtagtggc gctctccctg
    24181 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
    24241 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa
    24301 aacgcccatc gttaaaccta tataagcgat actaatcaaa tcctcaccgc gcctccctat
    24361 ccggtggcgc gaatgaacat cttgtcgact gtcaaatctg acacagatgt agccatccca
    24421 ttgcttaatc gaaaaaaaat catactatgc aggcgctaat tccaggatga taacgccttt
    24481 tgggctagtc atgctgctca taaatctggt atacttaccg ttacacattg gggctgattc
    24541 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
    24601 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
    24661 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
    24721 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
    24781 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
    24841 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac caaataatga
    24901 ttggacggtg taaggactac accaacaaaa acaggaagtt ataagtctca gcaggacacc
    24961 gaccagacgg tgaggagaca aaaaaggata cgcaacggag tcgcgactcc ctatgacatt
    25021 aaagcccgct gatgcgggct tttttattgg tcctgacccg ccctgaatag aattcatcca
    25081 attacagtcc agacatatac agtatccctc agctcagtat cagcttctcg acaaatggtc
    25141 actgttattt acccgtttcc agaaagagaa taatttgccc ccatagccag ccttcccgga
    25201 atctgtatgt tcattaatat tgttatcgat gccatcaagt aacataggtt ggatgcgatt
    25261 gatctta
//
LOCUS       DACADA010000063        25259 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.064, whole genome shotgun sequence.
ACCESSION   DACADA010000063 DACADA010000000
VERSION     DACADA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.064"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="ISB17_002437"
     CDS             complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="ISB17_002437"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAP1980362.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPHYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPIVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="ISB17_002438"
     CDS             complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="ISB17_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAP1980363.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="ISB17_002439"
     CDS             complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="ISB17_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAP1980364.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3735..3923
                     /locus_tag="ISB17_002440"
     CDS             3735..3923
                     /locus_tag="ISB17_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980365.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            3935..4687
                     /gene="bcsQ"
                     /locus_tag="ISB17_002441"
     CDS             3935..4687
                     /gene="bcsQ"
                     /locus_tag="ISB17_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAP1980366.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4684..7302
                     /gene="bcsA"
                     /locus_tag="ISB17_002442"
     CDS             4684..7302
                     /gene="bcsA"
                     /locus_tag="ISB17_002442"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HAP1980367.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7313..9652
                     /gene="bcsB"
                     /locus_tag="ISB17_002443"
     CDS             7313..9652
                     /gene="bcsB"
                     /locus_tag="ISB17_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAP1980368.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9659..10765
                     /gene="bcsZ"
                     /locus_tag="ISB17_002444"
     CDS             9659..10765
                     /gene="bcsZ"
                     /locus_tag="ISB17_002444"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAP1980369.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10747..14220
                     /gene="bcsC"
                     /locus_tag="ISB17_002445"
     CDS             10747..14220
                     /gene="bcsC"
                     /locus_tag="ISB17_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HAP1980370.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14302..16290
                     /gene="hmsP"
                     /locus_tag="ISB17_002446"
     CDS             14302..16290
                     /gene="hmsP"
                     /locus_tag="ISB17_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAP1980371.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16473..17759
                     /locus_tag="ISB17_002447"
     CDS             16473..17759
                     /locus_tag="ISB17_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAP1980372.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17979..19475
                     /locus_tag="ISB17_002448"
     CDS             17979..19475
                     /locus_tag="ISB17_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP1980373.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19571..20500)
                     /locus_tag="ISB17_002449"
     CDS             complement(19571..20500)
                     /locus_tag="ISB17_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1980374.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20732..21499
                     /gene="pdeH"
                     /locus_tag="ISB17_002450"
     CDS             20732..21499
                     /gene="pdeH"
                     /locus_tag="ISB17_002450"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAP1980375.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21569..23629
                     /locus_tag="ISB17_002451"
     CDS             21569..23629
                     /locus_tag="ISB17_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP1980376.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23808..25130)
                     /locus_tag="ISB17_002452"
     CDS             complement(23808..25130)
                     /locus_tag="ISB17_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1980377.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         5962 a         6436 c         7019 g         5842 t
ORIGIN      
        1 gggaaactct gcggcctttt tcgttcttac tgcgggtaag gcacccagtc gccgccgttc
       61 aggcgaacgt acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc
      121 ggtgctgttt gtggtaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa
      181 atcttgccat caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg
      241 tcgatgacaa tcggagcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg
      301 gggacgtctg tgatagacgg gctagggata tcacgtagac cagatacctg cattctgtcg
      361 cccttcagcg cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc
      421 tccagttcag taaagaaggc gtccagttca tcaaagaatt tttgcgcccg cgctttgtaa
      481 tccgctgttt tgctgacgcc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag
      541 aacgtggcac tacggctgtt tttatctttt tcggtaacgt ccagccaacg gttaagcacg
      601 gcggtatcgt cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt
      661 tgatccatca attcgctctg catgccgcca ttttcgcgaa cttccttcaa aaaaccaccg
      721 aactggccgt tatgtcccat catcaggtgc tgggtaaagc ccagtttcga caggttatca
      781 aacagatagc agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag
      841 ctggcgcgca gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc
      901 ttgaactcaa tatcgaaatg cgaccacagt ggatgcgaca tcaatccggc ggcttcaata
      961 tccgaccagg aaagcgaaca gatattaatc accagtagtt caaatggctg agcatcagcg
     1021 ggcagcgaag acgggaagat cgatttacgt ttcgcctccg cgttatagaa attattcagc
     1081 caggcgttaa ggtttgctgt tgttggcggt gcagtttgtg cgggcatatc acccactatc
     1141 ggtgcgccac ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg
     1201 gtcgttggtt gaccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc
     1261 agccatagca gaatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac
     1321 caggccacga ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca
     1381 agatcgatta aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt
     1441 tccggaccag gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc
     1501 cagtggcgca agcgatgcag gctatagtgc ggaatgggca tcagtagaaa cgcggcaaac
     1561 accaaattga ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc
     1621 agaaaataga agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg
     1681 gcggtatttt gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta
     1741 acgtcccggc cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa
     1801 tgcgtcgcaa agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga
     1861 caataatttc aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa
     1921 cagtcgcatt ggttctggta ttcgtcgcca gtggcgacca tcatgctcgg cattgatgac
     1981 cggtttagaa ctttgcgcta aaggcagcgg catgccccat tgttctgggg caagcaggcg
     2041 catctgcacc agctcggcac tgatttgatc atcttcaaac cagaccattc ggtttgagaa
     2101 aatgtcgcca gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg
     2161 acagaatgag agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg
     2221 gttagggcga catagcgtca gggcttgctc aacgcggata cccggtaccg gacgtagggc
     2281 aaccagaacg cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc
     2341 attacagaac acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag
     2401 caaggtagtg atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat
     2461 catcgtcaga cagcgggaga gtggcgcatt ccacgggata accatatttg caccgcaggc
     2521 caataataaa cgttcatcgg tggcgcgcag gctggcggta ttttcccgta cgaggatttt
     2581 catcgcacta ccgcgctggc gacgcagagt atgaatgctg cgggccagtg gctcgatttg
     2641 cgcattttgc tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa
     2701 caggacttcg ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag
     2761 tacagcaaca ttactcagaa tgcgtttttc gtcgctgcgt ggttggattt ccgcctcttg
     2821 gtgttgaact aatgtccagc aaccattttg ttgctgaacg ctaagttgct gacgggcgct
     2881 gacccctttt tcgttgcacc agaaagcaat atccagcaaa tgttggtcgc cctgaaaacg
     2941 caaactggca agaccaaaaa gtgaccgata ttcctcaagt aacaatgaaa attgtttatc
     3001 gttattattt ccggaattaa ttaccaaaag cgaacaatgg tttagccggc tccatttatt
     3061 cattttatcc aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cattatttgc
     3121 gcaaacaaga ataaaaaggt aattatgggg atcaatagaa cactgcaaat cacgggccaa
     3181 atagtataga cctttttcat gatttagcat cgaaaataat gttatttttt ccggcccttg
     3241 agaatcatct aattgaaaga ttttcgctgg atcgctgtcc atgctaatga ccgcgacgtg
     3301 tgcggtttca gcctgggatg caattgtttg attcgccaga ctgatagcat cttcatggcg
     3361 atcgacgtta aaccaccaga cgccgcctgc tggcatatgt cgcagctcat cccataatga
     3421 tgagataccg atagagaata cagggtccac aatgtccctc atgtcaacat tttgtctgat
     3481 tatcagttta ctagcgaaag ctaagaaata aacctaccat tgaaattaaa acttatcaga
     3541 tttagcatgt gaatcaattt agcaggtcac aagacatgtg attttagtct tttcgttttt
     3601 aactttttct gttgcataac ggaaagtcaa aaagtgagca cattcccgtc tcgccgtaaa
     3661 caataaaccg acgaaatgct cacagttaga attgtctcat gaacggtacg gttatttcat
     3721 agggatcaag aaaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct
     3781 atatcttcca gaatgatatt ttggcgttaa agcaggcatt ttcactgcct gatattgatt
     3841 atgccgatat ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg
     3901 cagagtttgc gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt
     3961 tcggggaggc gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt
     4021 gggagaaaat gtcctggtgg tcgatgccag cccggacaac ttgttgcgcc tgtcatttaa
     4081 cgttgatttt acccaccgtc agggttgggc cagagctatg ctggatggcc aggactggcg
     4141 tgacgccggg ttgcgctata cctcgcagct cgatttgctg ccttttggtc agttatcgat
     4201 tgaagaacaa gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt
     4261 acagcaacta aaagccagcg ggcgttacca gtggatttta attgacttac cgcgtgatgc
     4321 ctcgcagata acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt
     4381 ggatgccaac tgccatatcc gactgcatca gcaagcgctg ccggatggtg cacatatttt
     4441 gattaatgac ttccgtattg gcagccaggt tcaggacgat atttaccagc tttggttgca
     4501 aagccagcgc cgattgctgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgtct
     4561 ggcagctaag caaccagtcg gtgaatatcg cagtgatgcg ctggcggctg aagagatact
     4621 gacgctggcg aactggtgcc tgttgaatta ttccgggctg aaaacgccag tcgggagtgc
     4681 atcatgagta tcctgacccg gtggttgctt atcccgccgg tcaacgcacg gcttattggg
     4741 cgttatcgcg attatcgtcg tcacggcgcg tcggctttca gcgcgacgct cggctgtttc
     4801 tggatgatcc tggcctggat ttttattccg ctggagcacc cgcgctggca gcgtattcgc
     4861 gcagaacata aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg
     4921 gtccgttatc ttattcaaac atgctggtta ctgatcggtg catcgcgcaa agaaacgccg
     4981 aaaccgcgca ggcgggcatt ttcaggtcta caaaatattc gtggacgtta ccatcaatgg
     5041 atgaatgagc tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag
     5101 ctcggtcatt tgagtgccgg gacgcggcgg ttgatcctcg gtatcatcgt caccttctcg
     5161 ctgattctgg cgttaatctg cgttactcag ccgtttaacc cgctagcgca gtttatcttc
     5221 ctgatgctgc tgtggggggt agcgctgatc gtacggcgga tgccggggcg cttctcggcg
     5281 ctgatgctga ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc
     5341 tcgacgttaa actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct
     5401 gaaacgtacg cgtggattgt gctggtgctc ggctatttcc aggtcgtatg gccgctgaat
     5461 cgtcagccgg tgccattgcc gaaagatatg tcgctgtggc cgtcggtgga tatctttgtc
     5521 ccgacttaca acgaagatct caacgtggtg aaaaatacca tttacgcttc gctgggtatc
     5581 gactggccga aagacaagct gaacatctgg atcctcgatg acggcggcag ggaagagttt
     5641 cgccagtttg cgcaaaacgt gggggtgaaa tatatcgccc gtaccactca tgaacatgcg
     5701 aaagcgggca acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgatattc
     5761 gactgcgacc acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa
     5821 gagaaacagc tggcgatgat gcagacgccg caccacttct tctcgccgga tccgtttgaa
     5881 cgcaacctgg ggcgttttcg taaaacgccg aacgaaggca cgctgtttta tggtctggtg
     5941 caggacggca atgatatgtg ggatgccact ttcttctgcg gttcatgtgc ggtgattcgc
     6001 cgtaagccgc tggatgagat tggcggtatt gctgtcgaaa cggtaaccga agatgcgcac
     6061 acttctctgc gtctgcaccg tcgtggctac acctcagcgt atatgcgtat tccgcaggca
     6121 gcggggctgg cgaccgaaag tctctcggcg catatcggtc agcgtattcg ctgggcgcgc
     6181 gggatggtgc aaatcttccg tctcgataac ccgctcaccg gtaaagggct gaagtttgct
     6241 cagcggctgt gctatgtcaa cgccatgttc cacttcttgt cgggcattcc acgactgatc
     6301 ttcctgactg cgccgctggc gttcctgctg tttcatgcct acatcatcta cgcgccagcg
     6361 ttgatgatcg ccctgttcgt gctaccgcat atgatccatg ccagcctgac taactcgaag
     6421 atccagggca aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg
     6481 tatatcgcac caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc
     6541 accgccaaag gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac
     6601 atcttccttg ttctgctcaa cctggtgggc gtagcggttg ggatctggcg ctacatctat
     6661 ggtccgccaa ccgagatgct caccgtggtc gtcagtatgg tgtgggtatt ctacaacctg
     6721 attattctcg gcggcgcagt cgcagtatcg gtagaaagca aacaggtacg ccgatcgcac
     6781 cgcgtggaga tgacgatgcc cgcagcaatt gcccgcgaag atggtcacct cttctcgtgt
     6841 accgttcagg atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt
     6901 ctggaagggc agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg
     6961 gctcaggtgg cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcactacc
     7021 cagcaacata tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg
     7081 caggacagct acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc
     7141 ttccgtggct accgccatct ggcggaattt gcgccttctt cggtgaaggg catattccgt
     7201 gtgctgactt ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc
     7261 gaaacggcac aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag
     7321 aaaaatattc tggatttgtg cagtggctct ggggatgagt gcgttcccct ctttcatgac
     7381 gcaggcgacg ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccaggc
     7441 ggagcaaaat ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgcag atgcgccagt
     7501 cgtggcgcag aacggtcctt cccgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc
     7561 gccgggcagc atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg
     7621 cagcgatgaa gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcatt
     7681 actgcctgtc cagtcgcagt taaaggttta tctcaatgat gaactgatgg gcgtgctgcc
     7741 agtgaccaaa gaacagttgg gtaaaaaaac gctggcacaa atgcccatta acccgctgtt
     7801 tattactgat ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     7861 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     7921 ctatcagacc ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgaccc
     7981 gcgtgataac cgcaccaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgagct
     8041 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     8101 gaacttcccg gtgctctata accaactgcc ggatcgcaat gccattgtct ttgccaccaa
     8161 tgacaaacgc ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     8221 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     8281 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     8341 tgtggtagtg aatgaagtga aaccgctgct accgcgtaaa ccgtacgatg cgccgaactg
     8401 ggtgcgtacc gatcgtcctg tcacctttgg tgaactgaaa acctatgaag aacaattaca
     8461 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     8521 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     8581 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     8641 gagcagcaaa caggaggcga accgcctgct gctgcgtatt ccggtattac aaggtttgct
     8701 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagttgcg
     8761 gttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     8821 ccagccggtg cagaatcatg tggtgattgg tgacgactcc accatcgact tctcgaagta
     8881 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     8941 ccggatggcg gatttgtcgc aaaccatcac cgtgatgccg aaaacgccaa acgaagcaca
     9001 gatggaaacc ttgctgaata ccgttggctt tatcggtgcg cagacgggct tcccggcgat
     9061 taacctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     9121 cgtcggtggt atcccggaca aactgaaaga cgataagcag atcgacctgt tggtgcaggc
     9181 gaccgaaagc tgggtgaaaa cgccgatgcg ccagaccccg ttccccggta ttgtgccgga
     9241 cgagagcgat cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     9301 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     9361 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     9421 catgttcggt tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga
     9481 tgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     9541 tccgattctg ctggcggtgc tggcggctat cagtgtgata ttgctggcgt gggtactgtg
     9601 gcgtctgctg cgtattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     9661 gaatgtgttg cgtagtggac tcgtgacgat gctgctgctg gctgccttta gtgttcaggc
     9721 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     9781 cgtcatcgac cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggcat
     9841 gttctttgcc ctggcggcta acgaccgtgc agctttcgat aatcttctcg actggacgca
     9901 gaacaatctc gctcagggtt ctttaaaaga aaatttgccc gcctggctgt ggggcaagaa
     9961 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
    10021 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
    10081 cagcgcattg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggctc
    10141 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
    10201 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
    10261 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggctttt cgccagactg
    10321 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacgt tgatcagcag
    10381 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
    10441 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
    10501 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc
    10561 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
    10621 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
    10681 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
    10741 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
    10801 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
    10861 gaagcgaccc atcgtgaaga cctggtgcaa cagtcgttgt atcggctgga acttattgat
    10921 ccgaataacc cggatgtgat tgctgcccgt tttcgttctc tgttacgtca gggcgatatt
    10981 gatggcgcac aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
    11041 aaatcgtcgc ggactacgat gctgctttcc acgccggatg gtcgtcaggc actgcaacag
    11101 gcaagattgc aggcaactac cggtcatgcg gaagaagctg tggcgagtta caacaaactg
    11161 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
    11221 attccggctc gccgtggcga agcgattaat cagctaaagc gcattaatgc ggatacgccg
    11281 ggcaacacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
    11341 gaaggctttg ccgtcctgga acaaatggcg aaatcgaacg ccgggcgcga aggggcctct
    11401 aaaatctggt acgggcagat taaagacatg cccgtcagcg atgccagcgt gcaggcactg
    11461 aaaaaatatc tctcgatctt tagcgatggc gatagcgtgg ctgctgcgca atcgcaactg
    11521 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
    11581 gcggtggact ctggcatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
    11641 aacccgaaag acagtgaggc tctgggggcg ctgggccagg cgtattccca gaaaggtgat
    11701 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcgc tggatccgca caacagcaat
    11761 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
    11821 gatgccgcgc tgaaagccaa taatcctgac cgggcagaac ggctgttcca gcaggcacgt
    11881 aatgtcgata acaccgacag ctatgcagtg cttgggctgg gcgatgtggc gatggcgcgc
    11941 aaagattatc ccgccgccga acgctattac cagcagacct tgcgtatgga cagcggcaac
    12001 actaacgccg tgcgcgggct ggcgaatatt taccgccagc agtcgccaga aaaagccgaa
    12061 gcgtatatcg catcgctctc tgccagccag cggcgcagca ttgatgatat cgaacgcagc
    12121 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg gaaatgggcg
    12181 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagtgt atggattact
    12241 taccgacttt cgcaggatct ctggcaagcc ggacaacgca gccaggccga tacgttaatg
    12301 cgcaatctgg cgcagcagaa gccgaacgac ccggagcagg tttacgctta cgggctgtat
    12361 ctctccggtc acgaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg
    12421 cagtggaaca gcaatattca ggagctggtt aatcgactgc aaaacgatca ggtgctggaa
    12481 accgctaatc gcctgcgaga aaacggtaaa gaggcagaag cggaagcgct gctgcgccag
    12541 caaccgcctt cctcgcgtat tgacctcacg ctggctgact gggcgcagca gcgacgtgat
    12601 tacaccgccg cccgcgcggc ttatcagaac gttttgacgc gcgagcccac taacgccgat
    12661 gccattcttg gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc
    12721 cagctggcga aactgcctgc cactgataac gcctcgctga acacacagcg gcgcgtggcg
    12781 ctggcacagg cgcagcttgg cgataccgca gcggcgcagc agacatttaa taagttgatc
    12841 ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga tggtactgcg tgatggtgcg
    12901 aagtttgaag cgcaggcggg cgatccaatg caggcgctgg aaacctacaa agacgccatg
    12961 gttgcatccg gtgtgaccac gacgcgtccg caggataacg acacctttac ccggctgacc
    13021 cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc
    13081 tatcgccagc aggatcttaa cgtcactctc gagcacgatt actggggttc gagcggcacc
    13141 ggtggttact ccgatctgaa agcgcacacc actatgttgc aggtggacgc gccctattct
    13201 gacgggcgga tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact
    13261 aatgccgatg gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc
    13321 aaccgcagcc agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg
    13381 agctgggata tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc
    13441 agttacagcg atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcctatc
    13501 tccagttctt tgctggcctt tggtgggcaa aaagactctc cgagcaatac cgggaaaaaa
    13561 tggggcggcg tgcgtgccga cggcgtgggg ctaagtctga gctacgataa aggtgaagca
    13621 aacggcgtct gggcatcgct tagcggtgat cagttaaccg gtaaaaatgt cgaagataac
    13681 tggcgcgtgc gctggatgac gggctattac tataaggtca tcaaccagaa caatcgccgc
    13741 gtcacaatcg gcctgaacaa catgatctgg cattacgaca aagacctgag tggctactca
    13801 ctcggtcagg gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg
    13861 tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat
    13921 tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag
    13981 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggctttgg ctacacggcg
    14041 cgggcattac ttgaacgacg tgttacttcc aactggtttg tcggcacggc aattgatatc
    14101 cagcaggcaa aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
    14161 ggatggcagg gtgacatgga tttaccaccg cagccgctga taccttacgc cgactggtaa
    14221 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    14281 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    14341 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    14401 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    14461 cgcgaaccct tatcttctgc cattttgaaa ggcgatattc ccgaagcgga agctattctt
    14521 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc caaccagttc
    14581 caggcgctgc gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg
    14641 tttgagctac cggtgcaaat ctcgctgggc gtttactcgc tggaacgtcc ggcaaacccg
    14701 cagccaattg cctatctggt gctacaggcg gattccttcc gcatgtataa gttcgtgatg
    14761 agcaccctct caacgttagt gaccatttac ttacttttgt cgttaatatt gactgtggcg
    14821 attagctggt gcattaaccg cctgattttg catccattac gcaatattgc tcgcgaactt
    14881 aacgccatcc caccccagga tcttgttggt caccaactgg cattaccgcg tctgcatcag
    14941 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    15001 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    15061 gccttgctga tggagatgct ggagcaggtg gtggcgcgta aacaaaccac cgcgctgatg
    15121 atcatcacct gtgaaaccct gcgtgatact gcgggtgtgc tgaaagaggc gcaacgagaa
    15181 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg catgatcctc
    15241 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    15301 gctatcacat taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt
    15361 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    15421 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaaaaat
    15481 cagattcagt tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag
    15541 agcgatatcc ttaatgcact ggaaaatcat cagtttgcga tttggttaca gccacaggtc
    15601 gagatgacca gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    15661 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    15721 accgtcggtc actgggtgct ggaagagtcc tgtcgattgc ttgctgcctg gcaagagcgc
    15781 ggcattatgc tgcccttgtc ggtaaacctc tcagcgctgc aactgatgca tccaaatatg
    15841 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    15901 gaagtgacag aaagccgacg tattgacgat cctcatgctg cggtggcaat cctccgtccg
    15961 ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg
    16021 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    16081 gttgaaggtt tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    16141 ctgaacctac aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca
    16201 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    16261 ttcgaagaga gttacctgga agaaaaatag ctaccccaaa ctgattacaa aactttaaaa
    16321 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    16381 ggtacatttc tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccataacctt
    16441 acaagacctg tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt
    16501 actttcaggt cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa
    16561 taggcgagca aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg
    16621 ctcctgtcat cttctgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg
    16681 tcggtcgtac cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga
    16741 ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg
    16801 caacgcttga tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg
    16861 tcgccttcat tatggatgtc atcccggcga gcgtcattgg cgcatttgct agcggtaaca
    16921 ttctgcaggt actgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca
    16981 aaggccaact gatttttaac gtgatcgaaa gtttctcgca ggtcattttc ggcatcatca
    17041 atatgatcat gcgtctggca cctattggtg cgttcggggc aatggcgttt accatcggta
    17101 aatacggcgt cggcacactg gtgcaactgg gacagctgat tatctgtttc tacatcacct
    17161 gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct
    17221 tcaaatttat ccgttacatc cgtgaagaac tgctgattgt actggggact tcatcttccg
    17281 agtcggcgct gccgcgtatg ctcgacaaga tggagaaact cggctgccgc aaatcggtgg
    17341 tggggctggt catcccgaca ggctactcgt ttaatcttga tggcacatcg atatacctga
    17401 caatggcggc ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa
    17461 tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg
    17521 gttttatcgt gctggcagcg acgctctctg cggtgggcca tttgccggta gcgggtctgg
    17581 cgctgatcct cggtatcgac cgctttatgt cagaagctcg tgcgctgact aacctggtcg
    17641 gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac
    17701 tggacgatgt gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat
    17761 ctcaccactt atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc
    17821 tgtgcctgaa cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt
    17881 catgttcttg cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg
    17941 cagtttttta ttaccaggat agttgatcag gggttcacat gcagggcaca aaaattcgac
    18001 ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc
    18061 agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga
    18121 ctacacccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc
    18181 tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc
    18241 aaagcggtgg ccttgacgca gcgcaggcgc gttcattgtg gcagcagggg atcgacccta
    18301 aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc
    18361 ccaataaccg taacgacttg ctgaaagagg cgctctctta tctggcaaat gccactggca
    18421 aactgactat cacgccagaa accatcaacc atgcgttgca aagtcaggac atggtggcaa
    18481 cctggcctgc cgatactaaa gagggctggt ggcgttatcg tctgaaagga tcaaccttgt
    18541 taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaag attaaagatt
    18601 tctatcagaa atggtacacc ccggatgcaa tgacgctgct ggtggtggga aacgtggatg
    18661 cgcgctcggt cgtcgaccaa atcaataaaa cgtttggcga actgaaaggc aaacgtgaaa
    18721 caccggctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgaccg
    18781 acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg
    18841 aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgtgaggcg ctgttctggc
    18901 atgttcagca agcgttaagc gccagtaaca gtaaagacat cggtcttgga tttgactgtc
    18961 gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga
    19021 acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg
    19081 aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaag ctgtttgccg
    19141 cctatgcgcg agctgatacc gatattctga tggggcagcg gatgcgttcg ttgcaaaatc
    19201 aggttgtcga tatcgcgccg gagcaatatc agaaactgcg tcaggatttc cttaacagcc
    19261 tgacggtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa
    19321 tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg
    19381 atcaaatcat ggccccatcg actgcggctg ccgccacatc tgtcgccacg gatgacgtac
    19441 atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca
    19501 accatcaggt atttatggat gagctggata ggggggtaac actctatcca gccttttgca
    19561 tatgctgctt ttacgctggc atcgcttcac gcgggataat cgcgccgcga tactgaataa
    19621 cggtacttgc ggtcaggtgc ccgcgtttcg ctgcgtcttc cgcactgccg cccgtcaaac
    19681 gtaccgccag ataaccggca ctgaaagagt cgccagctgc ggtggtatcg atcacttttt
    19741 ctttcggcag ttttaccgcc ggaacatcca ctaacccttc accagcaatg gacaccaggc
    19801 aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa
    19861 tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc atcgtccagc gtcaggaagg
    19921 cgatatccgt gcattccagc atttgttggt acacctgctg tgtctcttct ttgctggccc
    19981 acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gccgttggcg cggcattcgc
    20041 gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc
    20101 tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgtcgcggat tgctcactct
    20161 ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc
    20221 tgtcggtttc aatgtagtaa aggcccggca gacggttttc catccgttgg gtcagggaag
    20281 tatcaacgtt ctcgccgcgc caggcgtcca gcatctgctg gctaaaactg tccgttccca
    20341 gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg
    20401 aagtgttcag ggtatcgccc ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct
    20461 caatcatgca ttcgccaatc acggcaatct ttttggacat agttgtgaac ctgatctgta
    20521 aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt
    20581 ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag tggggaaatt
    20641 taccccggcc aggcggtaaa gttctgcctt tgcgcgccga taatctttgt cgagtccggg
    20701 cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag
    20761 caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg
    20821 tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt
    20881 aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac
    20941 tgaaatcacc gttagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca
    21001 aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac
    21061 gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgaggagc ttccctggct
    21121 gcgtttcgaa ctggtggaac acatccgcct gccgaaagat tcaacctttg cctcgatgtg
    21181 tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact
    21241 aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca
    21301 gtcaccggaa ggacgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg
    21361 cggggtgatt gtcgagggcg tagaaacacc agaagagtgg cgtgatgttc agaactcgcc
    21421 cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatagaaa cgctgaatac
    21481 ggcggttctg gcgctataag ctgcctcatt ttccgcctgg ctggactatc tttaggactg
    21541 gcacagaaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat
    21601 ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg
    21661 gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc
    21721 tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag
    21781 ctgggtgccg tggccccatg tacacgcgga agacatcatt cttggcaatc caccggatat
    21841 tcccgaagtc acaatggtgc atttgccgcg agtagaagcg acgctggccc cgctggcgct
    21901 gctgaccaaa acggtctggt tgccgtggat caagctcgaa aagcccgacg cgcgcctgat
    21961 tcgcctctcc gaaaagaaca ataactggac gtttaatctc gccaacgatg acaacaaaga
    22021 cgcgaatgca aagccgtcgg cgtggtcgtt tcggctggat aatattctgt tcgatcaagg
    22081 gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg tcgatccgtt
    22141 aggcaagccg ctgtcgttca gcgaagttac cggatcgaaa ggtaaagcgg ataaagaaaa
    22201 ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac
    22261 tggtacggga aaaataggcg gtatgctggc gctgcgtggc gaaggaacgc cgtttccggt
    22321 acaggctgat ttccgctctg gtaacacccg tgttgctttt gatggcgttg tgaatgaccc
    22381 aatgaagatg gggggtgtcg atttacggct taaattttct ggcgattcgc tgggtgatct
    22441 ctatgaactg acgggcgttc tgctgcccga tacaccgccg tttgaaacgg atggtcggct
    22501 ggtagcgaaa atcgatactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg
    22561 cattggcgat agcgatatcc acggttctct gatctacacc accggcaagc cacgaccaaa
    22621 actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg
    22681 cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa
    22741 aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga
    22801 cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gcagcctgcc
    22861 gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct
    22921 gaaatttggc atagcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa
    22981 gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc
    23041 cgatgtagaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactgcgcgg
    23101 tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat
    23161 gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta
    23221 cattgtcggt gcgatatttg gtgatgatga ggtgcgggtg aactgcgcgg cggcgaatct
    23281 ggatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt
    23341 gattaacgtt accggtacgg caagttttgc ttcggaacag ctggatttga ctattgatcc
    23401 ggagagtaaa gggattcgga ttatcacatt gcgttcgccg ctgtatgtac gtgggacgtt
    23461 taaaaatccg caggctgggg tgaaagccgg gccgttgatt gctcgcggag ccgttgcggc
    23521 ggcactggca acgctggtaa caccggcggc ggcgttactg gcgctgatct caccttccga
    23581 aggggaggct aatcagtgca ggacaatttt gtcgcagatg aagaagtgat gataattgcc
    23641 ggatgtctga catccggcaa ttaaacagat ttagattgtt ggcaaagtgt gctttgttca
    23701 tgccggatgc ggcgtgaacg cctccggctt acaaatcctt gcgaattcaa tatattgtat
    23761 ggaaattctg gcctgataag cgtagcgcat caggcttttc actcttatta caacgactga
    23821 tgtcgcgtct catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga
    23881 tatagcccca ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat atacggcgca
    23941 acggaagccc cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact
    24001 tctgtcggga acagctctgg taacagcgcg cccatcggac cgaaggtcag ccccatcaga
    24061 cttaacccca gcagcagaaa ggcaaaaacc agaatcgggt tgccagaacc gagcaaaggg
    24121 gtaaaggcga atagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg
    24181 cgaccaaagg catcagccag taatccggcg actggcacca tcacgccaaa accaataact
    24241 gccatcatca gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg
    24301 gcggtactaa aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc
    24361 ataatgaacg tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg
    24421 atcttcacct gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac
    24481 acgcgaacat acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag
    24541 ccccagctca taaactgctc gtcggtcagt agccaggaaa gcagcaagaa agtgccgttg
    24601 gcaaagaaga agccaatcgg tgcgcccagc tgcgggaagg agccgtacaa tgcacgtttg
    24661 cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc cccattcacc acctaagccc
    24721 agaccctgac caaatcgagc cagcgccagc agcagcgggg cgaaaatacc aatcgtggca
    24781 tagcctggca gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc
    24841 agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg
    24901 cgcgcgacga aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg
    24961 ccctgcggga agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag
    25021 tcgaagaact caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg
    25081 atcggcgtgt attcttgctc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct
    25141 tattttttgg ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa
    25201 caaatcgcgg aaatggtcaa aaatcaggca aaaagggaat aatgtttagg ccagtgatt
//
LOCUS       DACADA010000064        24655 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.065, whole genome shotgun sequence.
ACCESSION   DACADA010000064 DACADA010000000
VERSION     DACADA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.065"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..389)
                     /locus_tag="ISB17_002453"
     CDS             complement(<1..389)
                     /locus_tag="ISB17_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HAP1980378.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGEQLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRG"
     gene            complement(392..826)
                     /gene="fliJ"
                     /locus_tag="ISB17_002454"
     CDS             complement(392..826)
                     /gene="fliJ"
                     /locus_tag="ISB17_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAP1980379.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(829..2169)
                     /gene="fliI"
                     /locus_tag="ISB17_002455"
     CDS             complement(829..2169)
                     /gene="fliI"
                     /locus_tag="ISB17_002455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAP1980380.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTRLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            complement(2162..2872)
                     /locus_tag="ISB17_002456"
     CDS             complement(2162..2872)
                     /locus_tag="ISB17_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HAP1980381.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(2876..3886)
                     /locus_tag="ISB17_002457"
     CDS             complement(2876..3886)
                     /locus_tag="ISB17_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP1980382.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINHFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            complement(3864..5510)
                     /gene="fliF"
                     /locus_tag="ISB17_002458"
     CDS             complement(3864..5510)
                     /gene="fliF"
                     /locus_tag="ISB17_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HAP1980383.1"
                     /translation="MNAQIKKLTQAFPAFRLHLVDNKRWVLMAGVGLAVAVTAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(5515..5856)
                     /locus_tag="ISB17_002459"
     CDS             complement(5515..5856)
                     /locus_tag="ISB17_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP1980384.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPSMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(5871..6857)
                     /locus_tag="ISB17_002460"
     CDS             complement(5871..6857)
                     /locus_tag="ISB17_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAP1980385.1"
                     /translation="MSELIATAASIINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETRDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            7244..8095
                     /locus_tag="ISB17_002461"
     CDS             7244..8095
                     /locus_tag="ISB17_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HAP1980386.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGFPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            8088..8459
                     /locus_tag="ISB17_002462"
     CDS             8088..8459
                     /locus_tag="ISB17_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980387.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            8456..9208
                     /gene="fliP"
                     /locus_tag="ISB17_002463"
     CDS             8456..9208
                     /gene="fliP"
                     /locus_tag="ISB17_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149597.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP1980388.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            9211..9483
                     /gene="fliQ"
                     /locus_tag="ISB17_002464"
     CDS             9211..9483
                     /gene="fliQ"
                     /locus_tag="ISB17_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HAP1980389.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            9485..10267
                     /gene="fliR"
                     /locus_tag="ISB17_002465"
     CDS             9485..10267
                     /gene="fliR"
                     /locus_tag="ISB17_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAP1980390.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHNLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            10257..11396
                     /gene="flhB"
                     /locus_tag="ISB17_002466"
     CDS             10257..11396
                     /gene="flhB"
                     /locus_tag="ISB17_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP1980391.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLSLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPKVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            11380..13473
                     /gene="flhA"
                     /locus_tag="ISB17_002467"
     CDS             11380..13473
                     /gene="flhA"
                     /locus_tag="ISB17_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP1980392.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(13693..14190)
                     /gene="rayT"
                     /locus_tag="ISB17_002468"
     CDS             complement(13693..14190)
                     /gene="rayT"
                     /locus_tag="ISB17_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAP1980393.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTQDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAEDVTDFSAG
                     ERIIL"
     gene            complement(14366..15115)
                     /locus_tag="ISB17_002469"
     CDS             complement(14366..15115)
                     /locus_tag="ISB17_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP1980394.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15427..16167
                     /locus_tag="ISB17_002470"
     CDS             15427..16167
                     /locus_tag="ISB17_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAP1980395.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16138..16905)
                     /locus_tag="ISB17_002471"
     CDS             complement(16138..16905)
                     /locus_tag="ISB17_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAP1980396.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(17110..17688)
                     /gene="lpcA"
                     /locus_tag="ISB17_002472"
     CDS             complement(17110..17688)
                     /gene="lpcA"
                     /locus_tag="ISB17_002472"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAP1980397.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            17928..20372
                     /locus_tag="ISB17_002473"
     CDS             17928..20372
                     /locus_tag="ISB17_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1980398.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(20415..20888)
                     /gene="ivy"
                     /locus_tag="ISB17_002474"
     CDS             complement(20415..20888)
                     /gene="ivy"
                     /locus_tag="ISB17_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAP1980399.1"
                     /translation="MGRISPGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            21042..21812
                     /gene="yafV"
                     /locus_tag="ISB17_002475"
     CDS             21042..21812
                     /gene="yafV"
                     /locus_tag="ISB17_002475"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAP1980400.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            complement(21911..21987)
                     /locus_tag="ISB17_002476"
     tRNA            complement(21911..21987)
                     /locus_tag="ISB17_002476"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21951..21953),aa:Asp,seq:gtc)
     gene            complement(22120..22851)
                     /gene="dnaQ"
                     /locus_tag="ISB17_002477"
     CDS             complement(22120..22851)
                     /gene="dnaQ"
                     /locus_tag="ISB17_002477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP1980401.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            22916..23383
                     /gene="rnhA"
                     /locus_tag="ISB17_002478"
     CDS             22916..23383
                     /gene="rnhA"
                     /locus_tag="ISB17_002478"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAP1980402.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(23380..24102)
                     /locus_tag="ISB17_002479"
     CDS             complement(23380..24102)
                     /locus_tag="ISB17_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1980403.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            24136..>24655
                     /gene="gloB"
                     /locus_tag="ISB17_002480"
     CDS             24136..>24655
                     /gene="gloB"
                     /locus_tag="ISB17_002480"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAP1980404.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMK"
BASE COUNT         5492 a         6622 c         6628 g         5913 t
ORIGIN      
        1 tgccccctaa tcacctcaat gatccccgtc gtcgataccg acggcgtgcg gggaaagtag
       61 accacctcac atacacacga gaaatcgtca aacttacctg cccagtcatc gcccatcacc
      121 aaaatatccg cactgtaccc gcgcaaatat tccgctttct gctccagcga ctcctccaga
      181 aatacgccat ccacgcacgc cagcccggcg ataatcgcca tgcggtcatc ctgagggtaa
      241 accggagccc tgcccttttt ggcgatattt aaggcatcgg atgatacgcc gaccagcagc
      301 tgctcgccca gcgtgcgagc gcgttgtaaa aggcgcagat gacccacatg aaacacatcg
      361 aaggtgccga aggtgatgac cgttttcatg gctactccgc taatttgtgc cgcaaccagc
      421 actggacggc gatgtcatcc accgcttttt gcgctttcgc cgtggcgctt tcctgttgct
      481 cgcggcgttt gtcgtccagc gtcagggcta ccactttctc ttcgcacgcc gccgccgtca
      541 ggtttttctg cattcgcgta gctttgatgt tggccagcgt tttttcctgc tcttgccagg
      601 cgatcacctt gcgcagtgtg cctttgtagc cgctgacgtt tttcagcgac tccaccgacg
      661 ccgccgcgcc cgcagatgtt ttctcgacaa ggtaacctaa tgctttgata ttgttatcat
      721 agccagcgca aatctgtttt tgctttgcca gctcgacggt tttgtctttg accgatttat
      781 cccgcaggcg ctgcaactgc gccagggtgt cgatgatttg tcgcatggtt attgcccttc
      841 tgcctttttg gcgatgggaa agaggatttg caggcggctt tgcaccagtt cgagggaggc
      901 gggttcgcgc atctcctggc gcaaaaatcg ctcgatagcc gggaacattt tcaccgcttt
      961 gtcgacgctg gcgtccgcgc ctgccacgta gccgccgagc gggatcagcg gtttgatctc
     1021 catataggcg gcgtagtttt gctttaacgc gcgggcggat tgctgatgtt ccaggcgggt
     1081 gacctggttc atgcagcggc tgatcgactg gccgatatca atagccggat agtgccccgc
     1141 ttccgccagt ttgcgggtta atacgatatg tccgtccagc actgcgcggg cgcagtcgac
     1201 tattggatct tgctggtcgt cgccttccgc cagcaccgtg tagatggcgg tcatcgatcc
     1261 tgagctttcg ctgttgcctg cgctctcgac cagtttgggg atcatgccga atgccgatgg
     1321 tggatagcct ttggtggcgg gtggttcacc gagtgagaga gcaatttcac gctgcgccat
     1381 ggcgtagcgg gtcagggaat ccaccagcag taaaacgtga tgcccgcggt cacgaaacca
     1441 ggcggcgatg gagtggcaca gttcggtggc tttcaggcgc atcagcggcg attcatcggc
     1501 aggcgcgaca acgacgatag actttgctag cccgtctgcg cccagcgagt ggtcgataaa
     1561 ctctttcact tcgcgcccgc gttcaccaat caacccgacc accacgatgt cggctttagt
     1621 ctggcgggtg atcatcccca gcagcacgct tttcccgacg ccgctgcccg ccatcagccc
     1681 gacacgctgg cctttgccga tggtcagcag gccgttaatc gctttcacgc ccacgtccag
     1741 cggttcgtcc actgaacggc gggttaaggg attgaccgag ggtggcagcg gcggcagaag
     1801 gtcgttgccg ttcaggcggc ctttgccatc cagcggttcg ccgagaccgt taaccacgcg
     1861 gcctaaccag ctttcgccaa tcaggatgtc gtgggctttt tcttccggga agacgcgtgc
     1921 gcctgccatc aggccgaccg gctgtttaaa cggcattaag taggtgatgt cgcgattaaa
     1981 gcccactgcc tgcgcgtcga tcagtgtgtg attggcgctt tcgatgcggc acagttgccc
     2041 ggtcattagc gggcagccga cgctctccag cagaatgccg ttcaggcgca ccagccgccc
     2101 ggcgacgcgg gccagcggga tcgactccag cgagcgcagg gcgttatcga aagcagaaaa
     2161 atcactcacc ctgcgactcc ggcgttagcg tctcttgcag cgcggtcatg cactgttcaa
     2221 ggcgatgctc acagccgatg tccagttcag atttgtcggt aatcacccgg cattcgcctg
     2281 gcggcagatc cggcgaaggc gtcagccccc attcgctgac tttttccggc accgcgtcgc
     2341 gcaggcggtt aaactcttct gtactcagca tcacttgcag ggtctccggc atcgtcggga
     2401 aggccgccag cgcctcttcc accagcgcca gtaactgcgt cggttgcagc gccagttcgc
     2461 agcggatcac ctgacgggtg actttctcca ccagttgcag caaatcttcc cgctgtttac
     2521 gttcaatatg cgcgaggaag tcgttcactt taccggtgat ggcttccagc ggctgcgccg
     2581 cctcaacaaa ctgtttgcgt ccttcctgct gcccggcgag gctgccttcg gtgtagccct
     2641 gacgccgccc ctcggcaaaa ccttgttgat gaccttcgct aaagccctgc tcctgacctt
     2701 cggtcattcc ctgagcaaac cctttttgca gcccttcctg aaaaccgtcc atcaactgac
     2761 gctggtaatc ggcggcgctg atactggcag taagcggttc tccggtcacc gcacgctggc
     2821 gcggcgggaa gcggtgcagg cgatagcgcc cgcgcagtgt ttcaatggtc atgaattact
     2881 ccgccgtccg ttccgcaaag agctgtagct gaatttcgcc cgcttcttcc agctcacggg
     2941 cgatgcccat gatttcgcgg cggatctgtt cgatacggct aaccggcacc ggacccagac
     3001 gcgcggtgat cgcttccagt tgctgcgcct gacgttttgg cattaccgcg taaatcgagc
     3061 ggcgcagcag ggcttcggtg cctttcagcg ccaccgccca gtcttccatc ggcacttcat
     3121 caagcagacg ccgacgcact tcttcgttct ggcggctgag gataaagaag tcgtacatct
     3181 catcctggat ctgccccaac acctcttcgt cgcgttcgcg catctggtcg aggatcacct
     3241 gctgattgcc ctggaagcgg ttgacgatat ccgccgcctg tttgatgcct tttaccttcg
     3301 agccatgttc cgaaagtacc gacaggccac gttcaatcag gcggtccagc tcatccacca
     3361 cgtcgcggtt gacgtcgttg agtttcgcca cccggtagag aatttcgttt tgcaccgatt
     3421 cgttgagata agagagcacc gctgccgaga tttccggcgt cagaaacgcg aggaacaccg
     3481 cctgcaattg cagatgttct tcggaaatca gcatcgccaa ctggcgtggt tccacccatt
     3541 gcaggcgcgc catccgcgag cgaatctcat cgccatagat gccgttgatc acgctgctgg
     3601 cgatctcggt acccaacgcc ttgttaagaa tgccctgcaa catggatcgc gatgcgccgt
     3661 tgatcccgct ctgttcgcgg aactcgtcaa agaagtggtt gatcacttta cgcgccatgc
     3721 tggttttgac gccggaaagt cgcgccatgt tttcgctcag gcgcaccact tcttcgcggc
     3781 tgagtttttg catcaccgtc gccgctgcct cttcgcccag acacagcagc aggatcgccg
     3841 cctgttccag gtagctgttg ctcttatttg ttgtcaattc gctcattgct ggtgatccat
     3901 tgtctgagaa cttcggctac gcgatcggtg tcgctcatgg ccagtttttg caggaactcg
     3961 agtttcactt cgaggccaga actttgtgac ggcaggctgt catcgccggg cagcgacggc
     4021 aggtcgacgt ttttgcgctc ctcttccggc atggcaatcc acggttcgct gacggcttcc
     4081 ggttctggtg tgataaccgg cgcacgtacc gccgtcagcc gtttcatcaa cggacgcacc
     4141 acgaagaaca gcagcaacag cgccagcagg ccgcagccaa tcagccgcac ccaggccagt
     4201 acgctgtcat ctttccacag tggaataacc ggttcgacag gcaccgcctg cggtacaaag
     4261 ttgagtaacg ataaggagag attgtcacca cgctgggcgt cgatcccggc ggcattgttc
     4321 agcaacgcgg tcagttgcgt ggtttgctcc ggcttccagt ttttcagcgc cggagcgttt
     4381 tgattgagta ccaccgccac gttgaggcgt ttcaccgcga agccgggatg ctgaatatgc
     4441 tggacgctgc ggtcgtaaga gtagtcgcgc ttactttcgc tgtgtttgga aagcgcttcc
     4501 ggcgagcggt tttcttccgt accgttggtc atctgattcg ctgcaatcgg cggacggtta
     4561 ctgagcgaac caggcacgcc catcgccacc tgattggtgt cgctgtccag cacgctctct
     4621 tcgcggttga ttttcggcgc gtcaccgtag tgttcctggg tttcgtcgat ggtgctgagg
     4681 tcgagatccg gcatcacact gacgcggtaa ttccccgtgc caaccagcga atccagcacg
     4741 ttcgccacgc tggcacgggt tttgtcctga atatctttga ggatctgatc gcgtttacgg
     4801 gtcgcggcag aaaccgcctc gcctgcgcca atgccgtcgg tcagcagatt tcccgcctga
     4861 tcgacgacgc taactttgct ggcctgtagc cccggaatac tgccggacac cagatgcaca
     4921 atggcgttca cctggtccat attcagcttc gcgccgtaat gcaagcgcac caccaccgag
     4981 gcgctgtttt gcggctcatc gctgaccaca aacgagcttt cttcgttgag cgccagatgc
     5041 acccgcgcgc tttccaccgc gtccagcgac atgatgctct gcgccagctc cccttcgaga
     5101 ctgcgtttat agcgcacgtt ctgcacgaac tgactgctgc cgagcacttc gtctttatcc
     5161 atcagctcgt agccgctggg caatatcgcc tgcacgcctt tcgcggcaag ggtcattcgc
     5221 gttttcgaca gtgcatcttc cggcaccaga atctgcccgc tctgcgggtc gatgcggtag
     5281 gtgagctttt cgccgtccag caccgtgaca atctgcgaca cgggcagatt ttcctggcgt
     5341 ccgtagagcg aaacgtagcc gcgattaccg gtccacagca cgctgacgat aatcgccgtc
     5401 accgccacgg caagccccac tcccgccatc aacacccagc gtttgttatc tacgagatgc
     5461 agtcgaaacg ccggaaacgc ctgggttaac ttctttattt gtgcattcat agggttagat
     5521 cgacatactc atcaggtcgt taaacccggt ggcgatttta ttgcgcacct gcaccagcgc
     5581 ggagaaagag agcgacgcct gctggctggc gatcatcgca cctgccaggt catcactttt
     5641 gcccgtgtcg acagccgttt gctgctgctc cgccacctgc tggaactgat caacgtgacc
     5701 aagcgcgccg tacataatgc ggttaaacga cacgtccggg tctgtggaac tgaaggtcat
     5761 ggagggtagc accggcgcct gggcgttggc ctgcatccgc tgcacgtcct gcatgatttg
     5821 cgcctgcatg gtgctgttga ttgtggtaat gctcattttt aacttccggg tcaggagaaa
     5881 acttcgatac cttgcttgcg catggaggcc agccgatagc gcagtgcgcg cggggtaatg
     5941 cctaacaggt cggcgatttt gctgcggttg ccctgatatt tacgcatcag gtcggcgata
     6001 tattgatact gcgcacttcg tccgtgctgg cctaaatcgc gggtttcggc aatgtggacg
     6061 gcgggctgaa caggccattg cagttcgctg tggtcggcaa tgtcagtttc cggcaggcct
     6121 aaggcatccg ggtaaatcac gccgtcgcgg tttaagatca ttcctctctg aatggcgttt
     6181 tccagttggc gaacatttcc gggccagcgg taattaagta gcgcccggcg tgtggattcg
     6241 gacagggtga tatttttcac cagcaccgtg gcatattttt taataaacga ctctgccagc
     6301 ggaataatat cgttcagacg ttcacgcagt ggcggcatgg ttataggaat aaccgccaga
     6361 cgataataaa gatcttcacg aaaacgtcca gcagccacgt cctgttccag atttttattg
     6421 gtgcaggcaa tcaggcgaaa attgagtttg atttgtctat tgctgcctaa tcgctcaacc
     6481 tgctgttcct gcaatacgcg taatattttg gcctgtaatg ccagcggcat atcgccaatt
     6541 tcatcgagca ataaggtgcc gttattagcc agttccattt ttcctggcac gctggcaatt
     6601 gcgccggtaa atgcgccttt gtcgtagcca aataaggtcg cttccagcat attttccggg
     6661 atcgccgcac agttcacgcc gatatagggc gcgttatcat tttcaccaaa ggctacggtg
     6721 tgaatatatt tcgccacgca ctctttcccc gcgccggttt cgccctgaat gagcaccggt
     6781 acgttaaagg cggcgacacg ctttgccaga gtaaaagcgt taatgatgga agcggcagtt
     6841 gcaataagtt cagacatagg gtgaacgata taagtagcga cagaaaacag gatgttctga
     6901 agcgtatcac cggtgggatt aacaagttcg aattaaacga taataaaaat attatttgtt
     6961 tataaacaac tagttagcaa tgtaggccgg ataaggcgtt tacgccgcat ccggcaattg
     7021 cgtgccgatg cctgatgcga cgctgcgcgt cttatcaggc ctacagtttg tgcaatgttt
     7081 tatgacgatg cccgatatct cgcccgttaa taatttaatt attttttcac cctattaata
     7141 atttgcccct ttcactcccc ctctatttaa tacctcacct tatgtcaccg ccggatttgc
     7201 gtgcacagaa acgcccatac cattacagca gagaaagtaa gccatgctga agtacagtaa
     7261 aacgccaggc atctttaaat tagaaggtaa tcggcttgga cgcccctacc atcgcctgcc
     7321 gaccctgttt acggggaatt ttgatgtaat tgattcgcat cttggcagct actttctcaa
     7381 aaagcatcgc agcaatatca ccctaaaaaa gattggctgc gaaatggata ttattaataa
     7441 aaatgccgag ctgatggttt cccaggtcgg gcatctggcg tttgatattg atcgctcctt
     7501 gctgttaatg ctgctgggca atttttatgg tctggagtcg tctcttgaag aagcgaaatc
     7561 acacaatgga ttcccgacca aaacggaaac acgtttaaaa aaccgcctcg cgctggatat
     7621 ctgcacgctg atttttaatc tgcaaacctc cggtattgcg ctgaaattaa aactggacag
     7681 cagcacggta attacccact gggcgtatca attaaccttt acccttgcgg gcgatgaaga
     7741 gagcagtttt cgtattttgc tcgacgacgc ccacaccgat tttattttga atctgatccg
     7801 ccacagcgag cacagtaaac cgcagcaggt ggcgaaatcc gttaataaac cggccctgat
     7861 taaagaaatc atccgctccc tgccgctgac cctgaacgtc aaaatcgccg agctatcgat
     7921 gaatgtcgcg gatctggcac agattaaagc cggggatatt ttacccatat cactgggaga
     7981 aacgttcccg gtggcgattg ggcaatctga attatttagc gccctgattg tggaagacaa
     8041 agacaaactg tttttgtcgg agctggcggg aaaaaatgag aacaaccatg agtaagcaag
     8101 aagatatttt agccggggat tttggcctca ccgatgaagt tgcgcctgtc gccaaatccg
     8161 ccgacgccga aacgctggtc acccgcctgg aagaccgatt ttccgactcg atgacgctac
     8221 tgaaacgcat tccggtgacc ttaacgctgg aagtatcgtc ggtggaaatc atgttagccg
     8281 acctgcttaa catcgacgac gacacggtga ttgaactgga taaactcgcc ggggagccgc
     8341 tggatattaa agtgaacaat atcctgctgg gcaaagcgga agtggtggtg gtcaacgaga
     8401 agtacggcct gcgcgtgctg gaatttaaca cccgcgatat caacgacctg gcgccatgaa
     8461 acgccgcact caactgacgc tgggcctggc gctgctggcg ctcgccccgc tggcgctggc
     8521 ccagggcggc gatatcgcac tgcttaacgt ggtcacccac ggcaacactc aggagtacag
     8581 cgtcaaaatt caggtactga ttctgatgac cctcgtcggt ttgctgccga cgatggtgct
     8641 gatgatgacc tgtttcacgc gttttattat cgtgctgtca ctgctgcgcc aggcgctggg
     8701 gctgcaacaa acgccgccaa accgcattct gattggtatt gcgctctcac tcaccctgct
     8761 ggtaatgcgc cccatctggc tcaatattta cgaccacgcg gtagtgccgt tcgaaaacga
     8821 tcagattacc ctgaccgatg cgttaagcac cgccgcgacg ccgctaaaac gctttatgct
     8881 ggcgcagacc gacaaaaaag cgatggcgca aatcatgacc atcggcggcg cgaagggcaa
     8941 tgccgccgac caggatctct ccatcgtggt gcccgcctat gtactgagcg agctgaaaac
     9001 cgccttccag attggcttta tgatttacat cccgtttctg gttatcgacc tgattgtcgc
     9061 cagcgtattg atggcgatgg ggatgatgat gctgtcgccg ctgattgttt cgctaccctt
     9121 taaactgatg ctgtttgtgc tgattgacgg ctggtcgctg accatcggca cgctcaccac
     9181 cagcattcgc gggctggggc tgggctgatt atgttgaccg tagacgtcgc cgccgacatt
     9241 gtcgccagcg gcattaaagt agtgattttg ctggtttcgg tactggtggt gccaagcctg
     9301 ctggtcggcc tgctggtgag cgtgttccag gcggtgacgc agattaacga acagaccctg
     9361 agctttttgc caaggctgat tgtcacgctg gtggtgctgg gcgtgtgcgg caaatggatg
     9421 attatccagt tgcatgatct ctgcattcac ctgtttaccc aggccgcgtt gctggttcat
     9481 tgagatgcgc acaagcgacg ttacgcaact gatggatctg gcgctggggc tgtggtttcc
     9541 cttcgtgcgc attatggcgt ttctgcgcta tatgccggtg ctggataaca gcgccctgac
     9601 cgcgcgcgtg cggattattt tgtcgctggc gctggcgatc atcatcacgc cgttgatccc
     9661 ccatcccatt ccccacaatc tgctatcgct caacagcctg atcctgacgg tggagcaaat
     9721 tttatggggc atgttattcg ggctgatgtt tcagtttttg tttctggcgc tacaactggc
     9781 cgggcaaatt ctctcgttca atatggggat gagcatggcg gtgatgaacg acccgagcag
     9841 cggcgcatcg accaccgtgc tggcggagct aatcaacgtc tacgcgatcc tgctgttttt
     9901 tgccatggac gggcatctgc tgctggtaag cgtgttgtac aagggcttca cctactggcc
     9961 gattggcaac gccctgcatc cgcaaaccct gcgtaccatc gcgctggcct ttagctgggt
    10021 gctggcgtcg gcatcgctgc tggcgctgcc gaccactttt atcatgctga ttgtgcaggg
    10081 ctgctttggc ttgctcaacc gtattgcgcc gccgctgaac cttttttctc ttggctttcc
    10141 gatcaacatg ctggcggggc tggtctgttt tgccaccctg ctctacaacc tgccggatca
    10201 ctatctgcat ctggcgaatt ttgtcttaca acaactcgac gcgctgaaag gtcactatgg
    10261 cggatagcag cagcgaagaa aaaacagaga aaccctcggc gcaaaagctg cgtaaagcgc
    10321 gtgaagaggg gcaactgccg cgctcgaagg atatggggct ggcggcgagc ctgtttgcgg
    10381 cgtttgtggt gatctccagc agttttccgt ggtatgccga ttttgtgcgc gagagcttta
    10441 tcagcgtgca tcagtatgcg caggagatta ataacccgca ggtgatcggc cagtttctgc
    10501 gccatcacct gttaattctc ggcaagttta tcctcacctt gctgccgatg cctgctgccg
    10561 cgctgctctc ttcgctggtg cccggcggct ggctgtttct gccaaaaaaa atcctcccgg
    10621 atttcagcaa aatcagtccg ttaaaaggca ttgggcggct gttttccagt gaacatctgg
    10681 cggaaacggg aaagatgacg gtgaagtctg tggtggtgct ggtgatgctg tgggtaagcc
    10741 tgcgcaataa ctttgccgcc tttctcagcc tgcaggcgct gccgttcaaa ctggcgataa
    10801 atgacggttt atcgctctac gccagcgtga tgcgcaactt tgtcattctg tttatcttct
    10861 tcgcgcttat cgacgtgccg ctggcgaaag ccctgttcac caaagggctg aagatgacca
    10921 aacaggagct gaaagaagag tacaaaaatc aggaaggtaa gccgaaagtg aaggcgcgtg
    10981 tgcggcgact gcaacggcaa ctggcgatgg ggcaaatccg caaagtggtg ccgaaagcca
    11041 acgtggtgat caccaacccg acccactacg ccgtggcgtt acagtatgac cagtcgcgcg
    11101 ccgccgcgcc gtttgtggtg gcaaaaggca ccgatgaaat cgccctctat attcgccagg
    11161 tcgccacgga gaatcaggtg gaagtggtcg agttcccgcg gctggcacgc tcggtttact
    11221 acaccaccca ggtgaaccag caaattccgt ttcagcttta ccgggccatc gcccacgttc
    11281 tgacctacgt cctgcaaatg aaacactggc gcgagggcgc acagccgcgc ccggccctga
    11341 acagacatat ttccattccc aaagaggtgc ttaaactgga tggcgaaaac aactaaatcg
    11401 ttccttgcgc tgctgcgcgg cggcaatctc ggcgtgccgc tggtcatact gtgtattctg
    11461 gcgatggtta ttctgccgct gccgcctgcg ctgctggata ttctgttcac cttcaacatt
    11521 gtgctggcgg tgatggtact gctggtggcg gtgtcggcga aacgaccgct ggaattcagc
    11581 ctgttcccga ccattttgct gatcaccacc ttaatgcgcc tgacgcttaa cgtcgcctct
    11641 acgcgcgtgg tgctgttgca cgggcatctc ggcgcgggcg cggcgggtaa ggtgatagag
    11701 tcgtttggtc aggtggtgat cggcggcaac tttgtcgtcg gtttcgtggt gtttatcatc
    11761 ctgatgatca tcaactttat cgtcgtcacc aaaggggccg agcgtatttc cgaggtttct
    11821 gcccgcttta ccctggacgc gatgcccggc aaacagatgg caattgacgc cgatcttaat
    11881 gccgggttga tcaaccaggc acaggcgcag acccggcgca aagatgttgc cagcgaggcc
    11941 gatttttacg gcgcgatgga cggggcatcg aagtttgtgc gcggggatgc catcgccggg
    12001 atgatgattc tggcgatcaa cctgatcggc ggcgtctgta ttgggatctt caaatacaac
    12061 ctgagcgccg atgctgcctt ccagcaatat gtgttgatga ccatcggcga cggcctggtg
    12121 gcgcagatcc cttccctgct gctctccacc gcggcggcga ttatcgtcac ccgcgtcagc
    12181 gacaacggcg atatcgccca tgacgtgcgt aatcagttgc tggcaagccc gtcggttctc
    12241 tacaccgcca ctggaattat gttcgtgctg gcggtggtgc cgggaatgcc gcatttcccg
    12301 ttcctgatgt tcagcgccct gcttggtttt accggctggc ggatgagcaa gcgcccgcaa
    12361 gcggcggagg cggaagagaa aagcctcgaa acgctaaccc gtacgatgac agaaaccagc
    12421 gagcaacagg tcagttggga aactattccg ctgatcgaac ctatcagttt aagcctcggc
    12481 tacaaactgg tggcgctggt ggataaagcg cagggtaatc cgctcaccca gcggattcgc
    12541 ggtgtacggc aggtgatatc cgacggtaac ggagttctgc tgccggagat ccgtattcgg
    12601 gaaaacttcc gcctcaagcc cagccagtac gccattttca tcaacggcat taaggctgat
    12661 gaagcggata ttccggcgga taaactgatg gccctgccct ccagcgaaac ctacggcgag
    12721 attgacggcg tactggggaa tgacccggcg tacgggatgc cggtcacctg gattcaaccc
    12781 gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc cagcgtgatt
    12841 gccacccatg tgaataagat tgtgcgcagc tatattcctg acctctttaa ctatgacgat
    12901 attacgcagc tgcataaccg tttgtcgtcg atggcaccgc gactggcgga agatttgagc
    12961 gcggcactca attacagcca gttgctgaaa gtgtaccggg cgctactgac tgaaggcgtt
    13021 tccctgcgcg atatcgtcac catcgccacc gtgctggtcg ccagtagtgc ggtgaccaaa
    13081 gatcatattc tgctggcggc agatgtgcgc ctggcgctgc ggcgcagcat tacccatccg
    13141 ttcgttcgca agcaggagct gacggtgtat acgctgaata atgagctgga aaatctgctg
    13201 accaacgtgg tgaatcaggc gcaacagggc gggaaagtga tgcttgatag cgtgccagtg
    13261 gacccgaata tgctcaacca gttccagagc acgatgcctc aggtgaagga gcagatgaaa
    13321 gcggcgggga aagatccggt gctgctggtg ccgccgcaac tgcgcccctt gctggcgcgc
    13381 tacgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt gccggatgag
    13441 ctggagttga agataatggg ggcgttgatg taggcatccg gcatcggtgc atattgccgg
    13501 atgcggcgtg aacgccttat ccggcctacg ggtgagcacg gttttgtcgg catgataaga
    13561 cgcgtagcgg cgcatctggc accggtgcac aactgccgga tgcggcgtga acgccttatc
    13621 cggcctacgg gtgagcgcgg ttttgtcggc atgataagac gcatctgcgt cgcatcaggc
    13681 atcggcgcac agctataaaa tgatacgctc cccagcacta aaatccgtta cgtcctccgc
    13741 ccaatcgatg ggatataacc ctcgcgcgac atcgcgatga aacgttgaga atggccaatc
    13801 actcacttgc tttacccaac catgttttac tggatttata taaatataat caacatgatg
    13861 gcgatagtct tgggtattgc gaatggcatg ctcccaaaaa cgcggttgcc agatattttt
    13921 caatccacaa gcatgggtaa attgcttttt aatttcccgc cagcgcgagg aaaaatcatc
    13981 atcgccttca ggtaatgtcc atatgcaatg catatgctcc ggcaaaacta cccaagcgtt
    14041 aatttcaaaa ggtctgtctc gcttaacgtt aataatagcg ttacggagcg tctgaaactg
    14101 ggtggtcaaa agttggtttc gacgattctg taaatttacc gtgaaaaacc aggttccccc
    14161 cttgatgtaa taacgacgat attcagacat aacgactcct ttttctcaac ggtaagaaga
    14221 aaaggaaaag tcggcaatga tgttgtgtta aatcgggaaa gcggctcgca aaaaatgcac
    14281 agatttatag gcatgataag acgcgtagcg tcgcatcagg caccggtaca caaccgccgg
    14341 atgcggcgtg aacgccttat ccggcctaca aaatcgtctc ttccgtcaaa atccgccgcg
    14401 cgcccaaaaa atgatcctgc cagaaaggtt cagctaaccg acttacccga atggtttccc
    14461 cggtgcgcgg tgactcgata aactgaccgt ctccaagata tacgcccata tggtcggcta
    14521 tctcacggct gtggatatgg aaaaacagca aatctccccg gcgcaggtcg ttattcgcca
    14581 caatcgttgc ccgacggtag tggtacatct cattggccgt gcgcgggagc ttagcctcaa
    14641 ggatcttgtt gtaggcataa aaaaccaacc cgctacagtc gaaaccttta tcaggccgcg
    14701 tgccccccca gacataaggc ttgccaagtt gctgttccag gcggtgaatc gccacttcgg
    14761 tgatgttatg caggtggttg ctgctgagga aatgattgtt ttccgccagc gcctgccagc
    14821 gtctgtcgtt cgccttgagc ggcgcaggaa accattctgg atgctgtttt atctgctccc
    14881 gattatgctg gcgcagcgcc cttctgcttt tagcattgcc ttccacaata tagctggcct
    14941 gctttttcag atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc
    15001 gggcggcgta gctaaaagag atatgtgatg tggtggcgct ggcgagtaaa gggagcaaaa
    15061 cgcataacga aaacacgcct ggatgcagaa atctgccgtg caagaacgaa gacatgaagg
    15121 acataaacca gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat
    15181 taattgctta tttgcatatt actattgcgg tttcataatc ttaggaaata agaaataaat
    15241 ctacaatcca tgcgcttttc ggactcttga aaactgcacc tgtgctaaat ttgtaaaccc
    15301 tgcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
    15361 tattccgatt tgggattcat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata
    15421 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccatg cgtttcgttt
    15481 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
    15541 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac
    15601 gtcaaaatgg gcgagcaata tcaactgctc gacagctata aaatctgtaa atattccggc
    15661 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
    15721 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattccca
    15781 aacgcctatg accgtgcgca tggttatgaa gggaaatacc tgatgattca cggcgattgt
    15841 gtttccatcg gctgctacgc gatgaccaat cagggtattg atgagatctt ccagttcgtt
    15901 actggtgcgc tggtgtttgg tcagccgagc gtgcaagtga gtatttatcc gttccgcatg
    15961 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcaactg
    16021 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat
    16081 ggccgttacg tggtcagcaa gccgttaagc cacgaagttg tgcagccaca actggcatca
    16141 aactacacgc tccccgaggc aaaataagcg ccattcacct ggcataatct tttgccaggt
    16201 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
    16261 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
    16321 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtcagaaag
    16381 cagcatgttg aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa
    16441 cactgccgcc atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca
    16501 gcaaaaggct ttttcgctat cggtttcacc gaccgggcgg aagttgccgg tttccagtga
    16561 tttgtagccc gtcagttgtc cattatgggc ataagtccag ttacgccccc ataactcgcg
    16621 ggtaaacggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
    16681 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
    16741 gctgggttgc ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
    16801 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
    16861 gatatcggta gggacgttgg cactcatccc gagcaattcg cacatgggta acctccacaa
    16921 cagcgggagc gcatggcccc cggcgaagat tgatttaaga attttcaggt cggatgtggc
    16981 gtgaacgcat tattcggcct acggttcggg tgcagattgt aggcatgata agacgcgtca
    17041 gcgtcgcatc aggcatcaga gcacaaatgc cggatgcggc gtaaacgcct tatccggcct
    17101 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
    17161 aatgtggatc tcctgaatgc ggtcggcata accaaagtgc ggtacgcgaa tttcgatatc
    17221 cgccgtgcca gccattttgc cgccgtcttt accggtcagg gtgatcactt tcatcccctt
    17281 ctcacgcgcc gccgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
    17341 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtaa cgggagaaaa tatcattgaa
    17401 accaaaatcg ttaccgacgc aggaaatatg actaacgtca gaaatggcaa tcgccgggta
    17461 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tggcgtcgca
    17521 atgggagccg ccgttgccac aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
    17581 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
    17641 cgtttccgcc gcttcgttca gttcattacg aataagatcc tggtacatga ggatatcctt
    17701 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
    17761 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
    17821 agctttgttg taattatatt gtaaacgcat tgctaaatgt ttttacatcc actacaacca
    17881 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
    17941 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
    18001 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgcgggt ctgtggtcgg
    18061 cgtgggtact agtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
    18121 agtcgatgat ttccgcaccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
    18181 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
    18241 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gctgaagagc
    18301 aagcgtttct cgacggtccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
    18361 ctcatgagct ggcggacctg ccgccggagt tgtgggcgta ccttaaagag catcgtttct
    18421 tcgcgatgat catcaaaaaa gagtacggcg ggctggagtt ctcggcttat gcccagtctc
    18481 gtgtgctgca aaaactctcc ggcgtgagcg ggatcctggc aattaccgtc ggcgtgccga
    18541 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaaaatcact
    18601 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgacc agccccgaag
    18661 cgggttccga tgcgggcgcg attccggata ccgggattgt ctgcatgggc gaatggcagg
    18721 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga
    18781 ttgcgaccgt gctgggtctg gcgtttaaac tctccgaccc ggagaaatta ctcggcggtg
    18841 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
    18901 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
    18961 tgttcgtgcc gatcgattac atcatcggcg ggccgaaaat ggccgggcaa ggctggcgga
    19021 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
    19081 gcgtgaaatc ggtagcgctg gcaacgggtg cgtatgcgca cattcgccgt cagttcaaaa
    19141 tttctattgg taagatggaa gggattgaag agccgctggc gcgtattgcc gggaacgcct
    19201 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggt gaaaaacccg
    19261 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcaa tcgattatcg
    19321 acgcaatgga tatcaccggg ggtaaaggca ttatgctcgg gcagagcaac ttcctggccc
    19381 gtgcttacca gggggcaccg attgccatca ccgttgaagg ggcaaatatt ctgacccgta
    19441 gcatgatgat cttcggtcag ggggcgattc gttgccatcc gtacgtgctg gaagagatgg
    19501 aagcggcgaa gaacaatgac gtaaacgcgt tcgataaact gctgttcaaa catatcggtc
    19561 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
    19621 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
    19681 acctcgccct gctttctgat gtctcgatgg cggtgctggg cggcagcctg aaacgtcgtg
    19741 agcgcatctc agcccgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc
    19801 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
    19861 tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa aacttcccga
    19921 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
    19981 caccgtctga caagctggat cataaggtgg cgaagatttt acaagtgccg aatgccaccc
    20041 gttcacgtat tggtcgcggt cagtacctga ctccaagcga gcataacccg gttggtttac
    20101 tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg atctgtaaag
    20161 agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac gcgctgacga
    20221 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
    20281 gtagtattaa cgttgatgac tttgatccag aagagctggc gacgaagccg gtaaagttgc
    20341 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
    20401 tttttatcgg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
    20461 aacgccgcga acagcaccgt tttgccatca atcgaaagcg catcattcac attcagccag
    20521 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
    20581 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgagg tttgcacgcg
    20641 ctcatcacct gatacgtctc atctcccaat gtcacggttt gtgcgggagt ataagtaccg
    20701 cctttcatca cccaggcagg cagcttatgc ccttgcacca tctgattaaa cgccgctttg
    20761 gtggtttcgc cctttgcaag actgctaatg gttaaatcat cctgcgccat tgcactggtg
    20821 gcgatgacca gagcggcgac tgtcgttatt gccttaaaca tcattcctcc cgggcttatc
    20881 ctgcccatgt gattaactcc ctaaagcctg gcgataacac gataatttgc aaatcagata
    20941 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
    21001 gttagagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
    21061 cgcttttgca gcaaccactg gtgtggatgg atggtcctgc caacctgcgc cattttgatc
    21121 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg
    21181 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg aactggatga
    21241 ccgctaaagc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
    21301 ctgattcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
    21361 agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcaggc aatgagcgag
    21421 tgattgtgga atggcgcggc tggcgtattt taccgctggt gtgctacgac ttacgttttc
    21481 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
    21541 caccgcgctc tctgcactgg caggcattac tgacagcccg cgcgattgag aatcaggcgt
    21601 atgtagcggg atgtaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
    21661 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
    21721 gcattgatgc ggagctatcg atgatggcat tgcgggaata tagagaaaag tttccggcat
    21781 ggcaggatgc ggatgagttc acatttcgtt agagggtgaa tatggctacg agataagttt
    21841 cgtggctatt tttctctgga acttaaacga cgaaatcccg aatcagtaat gattcgggct
    21901 ttctgaatat tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
    21961 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
    22021 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa
    22081 atcgcccaag tcgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcaggt
    22141 tccgcccttc ttctgcacca gatcaagacg ggcttcatgt gctgcaagct cttcatctgt
    22201 cgcaaaaaca acgcgtaact tacttgcctg acgcacaatg cgctgaattg ttgtttcacc
    22261 ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
    22321 cgccagataa acttccgcaa ggatctgggc atcgagtaat gccccatgca gcgttcgttt
    22381 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa
    22441 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat
    22501 atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcaaacg ctgcgttatg
    22561 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctacct cggcaaacgt
    22621 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
    22681 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
    22741 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
    22801 accggtggtt tcagtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
    22861 ttatgtcaga cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
    22921 taaacaggta gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta
    22981 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgctggct acacccgcac
    23041 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
    23101 ttgcaaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
    23161 ccataactgg aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga
    23221 tctctggcaa cgtcttgatg ctgctctggg gcagcatcaa atcaaatggg aatgggttaa
    23281 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat
    23341 gaatcccaca ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgaca
    23401 ttgccgggtg gcaccaaccg cctggcgaat tcgcggttta ttcttgctct gtttcatcgg
    23461 atttagcgtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
    23521 aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
    23581 gctggcgtgt agcacttcaa aattcaacaa agagagccag tccagctgcc gcatcagagt
    23641 aaacatccgg ctgttatagg gcgatgtttt gcgcaatacc ggcacaagtt tgcgtaatcc
    23701 cattaaactg atgggattga agccactaat caccagccaa ccatcatcaa tcagtacccg
    23761 atcggcttca cgcaataaac gatgcggatc agtgcaccac ggcaatgtat gtgccagtag
    23821 acaaacatca acggatttat cggcaaaagg aagatgaagt gggtccgcct gcacctggac
    23881 gggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
    23941 ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg
    24001 gttgagctga cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagca
    24061 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
    24121 actgagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
    24181 gttttgaatg atgaagcagg tcgctgtctg attgtcgatc ccggggacgc agagccagta
    24241 ttaaacgcca tttccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcac
    24301 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat
    24361 ggaccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
    24421 ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc
    24481 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
    24541 cggttgtttg aagggacacc atcacaaatg tatcaatcac ttagaaagtt aagtgcgtta
    24601 cctgacgaca cattggtatg ttgtgctcat gaatatacct tatcaaatat gaagt
//
LOCUS       DACADA010000065        24173 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.066, whole genome shotgun sequence.
ACCESSION   DACADA010000065 DACADA010000000
VERSION     DACADA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.066"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..523
                     /locus_tag="ISB17_002481"
     CDS             <1..523
                     /locus_tag="ISB17_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAP1980405.1"
                     /translation="GYGHTKLVAEKVAQGAHADLISIDNEGNITEQEWEKLDAADAII
                     FGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDKQVTLIYLQTL
                     ASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQIPTGDLDTAVK
                     YGERVATITARLK"
     gene            complement(694..1686)
                     /gene="rpoS"
                     /locus_tag="ISB17_002482"
     CDS             complement(694..1686)
                     /gene="rpoS"
                     /locus_tag="ISB17_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAP1980406.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1749..2888)
                     /gene="nlpD"
                     /locus_tag="ISB17_002483"
     CDS             complement(1749..2888)
                     /gene="nlpD"
                     /locus_tag="ISB17_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAP1980407.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3028..3654)
                     /gene="pcm"
                     /locus_tag="ISB17_002484"
     CDS             complement(3028..3654)
                     /gene="pcm"
                     /locus_tag="ISB17_002484"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAP1980408.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3648..4409)
                     /gene="surE"
                     /locus_tag="ISB17_002485"
     CDS             complement(3648..4409)
                     /gene="surE"
                     /locus_tag="ISB17_002485"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAP1980409.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4390..5439)
                     /gene="truD"
                     /locus_tag="ISB17_002486"
     CDS             complement(4390..5439)
                     /gene="truD"
                     /locus_tag="ISB17_002486"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAP1980410.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5436..5915)
                     /gene="ispF"
                     /locus_tag="ISB17_002487"
     CDS             complement(5436..5915)
                     /gene="ispF"
                     /locus_tag="ISB17_002487"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAP1980411.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5915..6625)
                     /gene="ispD"
                     /locus_tag="ISB17_002488"
     CDS             complement(5915..6625)
                     /gene="ispD"
                     /locus_tag="ISB17_002488"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP1980412.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6644..6955)
                     /gene="ftsB"
                     /locus_tag="ISB17_002489"
     CDS             complement(6644..6955)
                     /gene="ftsB"
                     /locus_tag="ISB17_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAP1980413.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7148..7471)
                     /locus_tag="ISB17_002490"
     CDS             complement(7148..7471)
                     /locus_tag="ISB17_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAP1980414.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7520..8125)
                     /gene="cysC"
                     /locus_tag="ISB17_002491"
     CDS             complement(7520..8125)
                     /gene="cysC"
                     /locus_tag="ISB17_002491"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAP1980415.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8125..9552)
                     /gene="cysN"
                     /locus_tag="ISB17_002492"
     CDS             complement(8125..9552)
                     /gene="cysN"
                     /locus_tag="ISB17_002492"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAP1980416.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9554..10462)
                     /gene="cysD"
                     /locus_tag="ISB17_002493"
     CDS             complement(9554..10462)
                     /gene="cysD"
                     /locus_tag="ISB17_002493"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAP1980417.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10714..11751
                     /gene="iap"
                     /locus_tag="ISB17_002494"
     CDS             10714..11751
                     /gene="iap"
                     /locus_tag="ISB17_002494"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAP1980418.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11877..12029)
                     /locus_tag="ISB17_002495"
     CDS             complement(11877..12029)
                     /locus_tag="ISB17_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1980419.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12295..13029)
                     /gene="cysH"
                     /locus_tag="ISB17_002496"
     CDS             complement(12295..13029)
                     /gene="cysH"
                     /locus_tag="ISB17_002496"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1980420.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13103..14815)
                     /gene="cysI"
                     /locus_tag="ISB17_002497"
     CDS             complement(13103..14815)
                     /gene="cysI"
                     /locus_tag="ISB17_002497"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAP1980421.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14815..16614)
                     /gene="cysJ"
                     /locus_tag="ISB17_002498"
     CDS             complement(14815..16614)
                     /gene="cysJ"
                     /locus_tag="ISB17_002498"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAP1980422.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            16930..17295
                     /gene="queD"
                     /locus_tag="ISB17_002499"
     CDS             16930..17295
                     /gene="queD"
                     /locus_tag="ISB17_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAP1980423.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            17373..18644
                     /locus_tag="ISB17_002500"
     CDS             17373..18644
                     /locus_tag="ISB17_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1980424.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18635..18895
                     /locus_tag="ISB17_002501"
     CDS             18635..18895
                     /locus_tag="ISB17_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP1980425.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18912..19487
                     /locus_tag="ISB17_002502"
     CDS             18912..19487
                     /locus_tag="ISB17_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAP1980426.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19635..20495)
                     /locus_tag="ISB17_002503"
     CDS             complement(19635..20495)
                     /locus_tag="ISB17_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP1980427.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20492..21271)
                     /locus_tag="ISB17_002504"
     CDS             complement(20492..21271)
                     /locus_tag="ISB17_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAP1980428.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(21249..22658)
                     /locus_tag="ISB17_002505"
     CDS             complement(21249..22658)
                     /locus_tag="ISB17_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980429.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22680..24134)
                     /locus_tag="ISB17_002506"
     CDS             complement(22680..24134)
                     /locus_tag="ISB17_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP1980430.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKKVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         5657 a         6627 c         6082 g         5807 t
ORIGIN      
        1 aggatatggt cacactaaac tggttgctga aaaagtggca caaggcgccc atgcagattt
       61 aatttcgatt gataacgaag gaaatatcac tgagcaggag tgggaaaaac tggacgcagc
      121 cgatgccatt atcttcggtg ctcccaccta tatgggcggt gcgccatggc aattcaagaa
      181 atttgctgat gccagttcca aagcgtggtt tacacgtaaa tggcaggata aagtcttcgg
      241 gggtttcacc aacagcgcca gcctgaatgg cgataaacaa gttacgctga tctatctgca
      301 aacgctggct tcacagcatg gcggtctgtg ggtgagttta ggtcaggctc ctgccaatgt
      361 gcttgcgtca accagggaag atgtaaacaa tctgggcggt tcggtcggtt tgctggtgca
      421 atctccagcc gatgcgggtg cagagcaaat ccctacgggc gatctggata cagccgtgaa
      481 atatggtgaa cgcgtggcga caattactgc gcgcttaaaa tgatgattgc cgaatgtgat
      541 gctgacgcgt ctatccggtc ttctacatct aaaaagtgcc gtcccctggt cagtcagata
      601 aagttttaac gctgtatccg ataacagttc aacatgtacc aaaagaaaaa aagccagcct
      661 cgcttgagac tggcctttct gacagatgct tacttattcg cggaacagcg cttcgatatt
      721 cagcccctgc gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg
      781 aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta
      841 ccccagcaaa ccgaatcgac gtgccagcac ttcacgctgt ttggcgttca gctcgaacag
      901 ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccattctc
      961 tttttcatcg gccaggatgt ccagcaatgc tttttcggaa tcaccaccca gcggggtgtc
     1021 taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc
     1081 cagttgctct gcgatctctt ccgcacttgg ttcgtggtcc agcttatggg acaactcacg
     1141 tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg
     1201 ggtttggttc ataatcgccc gttcgatcgt ctggcgaatc caccaggttg cgtatgttga
     1261 gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaggttgcc
     1321 ctcttcgatc aggtccagca acgccagacc acgattgcca taacggcggg caatttttac
     1381 caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc
     1441 gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc
     1501 aaggtaaagc tgagtcgcgt ccagcacacg ctgtgtggct ccctgcgata acagttcctc
     1561 ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc
     1621 aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg
     1681 actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt
     1741 ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga
     1801 atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc
     1861 cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc
     1921 aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttaccg
     1981 gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata
     2041 tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc
     2101 ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt
     2161 gtgcttgctg ttggtaccgt tacaggcact gtgaccgtgg tcgcagttgg cttgttgttc
     2221 ggcaacattt tgttagcact ctgttcaccc gaagactcag aatacgtaat tgtcggttgc
     2281 gacgcaacag caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg
     2341 tcggcctggg taatggcatt tccgcctgtg attggcgtac cggaagcatt acccacctgt
     2401 aaggtctgac cgacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca
     2461 cggaaatcgt tgccagtaat ccaggcgata tagaaaagcg tgtcgccttt tttcacggtg
     2521 tatgtactgc cgctataact gcctttcgga atgttcccat actgacggtt gtagacgatt
     2581 cgtccgtttt ccatctgtac cggctgctga gctactggct gcacaggctg gatttgcggt
     2641 tgttgagtag cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgcgctgta
     2701 gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattggt atttgcgggc
     2761 gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc
     2821 agccatagcg aaaccagtga caatgccgca atgcggcgaa cggtgaattt tgggcttccc
     2881 gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag
     2941 gcaccatact gaacactgga aaagatgttc acgatacgct gacttgcggc aaaataacca
     3001 ggaaaaatcc aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa
     3061 gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa
     3121 atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat
     3181 tagcgcagtt ggaatttcag gcggtgccgc cgtaacaatg atagcgtcaa acggcgcgcg
     3241 tgcctgccaa ccttgccatc catcgccatg acgggttgaa acattatgta aatcaagatt
     3301 tttcaggcgg cgacgcgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg
     3361 ctggacaaga tgcgccagga ttgcagtttg atatcccgaa ccagtgccaa tttccagcac
     3421 ccgcgactgc ggcgttagct cgagtaattc ggtcattcgc gccaccatat acggctgcga
     3481 aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc
     3541 ttcatcaacg aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg
     3601 aataccttgc gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg
     3661 ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa
     3721 tccacatgca gcggcgtgat ggaaacatag ccctcatcta ccgcagcaaa atcggtcccc
     3781 ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga
     3841 tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata
     3901 cctttgattt gatccaatgg taaatccgga acgttaatat taagaatacg cccggtgcgc
     3961 agcggctctt tacacaatgc gcgcaaaatt gagcaggtta ccgccgcggc agtgtcgtaa
     4021 tgtttatgcc cgtcaagcga gacggcaagc gccggaaaac ctaaatgacg gccttccatc
     4081 gcggccgcta ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt
     4141 ccggacacaa caatgtccgg gcgcgggcgc atcagagcat ttacgccaag atagacgcaa
     4201 tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg
     4261 gaagattcca gtgtcagaga attagaagcg ccgctgcggt tacgatcggg ggcgaccacc
     4321 tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc
     4381 ccgtcatcat tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttccctgac
     4441 aacgctggtt gcaaaacttc ccgccggaag ccagaaacgg atttctacgg tgacgtcatc
     4501 ccaccagttc cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac
     4561 tttttcacgc accagtaaag tttgtaattc agtttcttcg gcgacagctg cttgttcgaa
     4621 tgccagcgct tcacgctgag ttccccattc gccactgccc agcaatgcgg cggttatcat
     4681 tagctcttta tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgcaacaaa
     4741 ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc
     4801 ttttttgagg cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca
     4861 aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc
     4921 ctgcaagttg ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt
     4981 tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac
     5041 cagggtaaag gcgttacctt tcagcgcgcc taaacgcagc ttgcgcttgt gccgcgcata
     5101 ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcatct ctttgcccgg
     5161 cacgcgagcg cataaccact gttccgtaac agcatgttta tctttttgcc cagcgaagct
     5221 gacttcacgg gcatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt
     5281 gcagccattt ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc
     5341 caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg
     5401 tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc
     5461 gccaccgctt cacaggcaat cccttcccca cgtccggtaa atcccagttt ttccgtagta
     5521 gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc
     5581 atttgtggaa tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg
     5641 ccgagggcat aacctttcgc ctgaatacga cgccaggctt cgcgcaacag ctcgcggctg
     5701 tcggcacctt taaatgccgg atcggtatcc gggaacagct tgcctatatc tcccagcgcc
     5761 gccgcgccaa gcaatgcatc ggtcaacgca tgcagcgcca cgtcgccatc agaatgcgcc
     5821 agcagccctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca
     5881 aaggcgtgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc
     5941 gggtgaggta aaattcggcc agcgccaaat cctccgggcg cgttaccttg atgttatccg
     6001 cgcggccttc aacaagttgc ggatgaaaac cgcaatattc cagcgccgag gcctcatcgg
     6061 taatggtagc gccttcattt agagcgcgcg tcaggcagtc atgtaacagc tcacgaggga
     6121 aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat
     6181 ttttgcccgg ttcggcacgt ttcatcgtat cgcgcaccgg cgcagcgagg atccctcccg
     6241 tgcggctggt ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc agacaaggac
     6301 gagcggcgtc atgcaccaat acccactgcg cgtcgccagc ggctttcaga cctgccagca
     6361 cggaatcggc acgctcttca ccaccatcaa caaccgtgat tcgtggatga ttcgccagag
     6421 gaagttgtgc aaagcggcta tcgccaggac ttatggcaat gacgacacgt ttcacccggg
     6481 gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat
     6541 attgcttagg acattccgtt tgcattcggc ggccaaatcc ggccgccgga accacggcgc
     6601 aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc
     6661 ccgcagactg tgcgcgcttt gacgcgtcag gcaccagacg ataaaacgtt tcgcccggcc
     6721 tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat
     6781 cgtcaatttc ggcaaaaagc tgatcgtttc gcgctttaag tttcgcgttt gtagcttgct
     6841 gtgccgccac atcattattg acgcgagtat agtcatgtat accgttctta ccgaaccaca
     6901 gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc
     6961 cccctgaaaa acggcatctt catcccatgc atccgaagac gactctacat cctctgttgg
     7021 ggataccgcg acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg
     7081 cgtgcagaaa ataatctcat tattagttac ggattgaatt atgaacagaa tagacgggaa
     7141 agaacaatta acccaatagc cacataaaca gtgcaccaaa cataatgcca accgtcgcca
     7201 gagtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagt gcaatcccca
     7261 ccactacagc gacgggcatc agcgccagaa agaaaggcca ggtgtagata aaaaagagca
     7321 gcgtgttaga gccataaatc aacatcggaa ttgccaacgc gcacagccac gataaaaaac
     7381 caaccacagc gcctggcagc gaccatgtgg tgtcttcaac ctcagtaagg ctgtcgttat
     7441 ttgttaatgt aatgttgtgg ctgttacgca tatctgatcc tgttactttg acgaaccggg
     7501 catgaaaccc ggtggtgtct caggatctga taatatcgtt ctgtctcaac agatctaata
     7561 attgctgtac caaatttgtt actaattgtt caccattgag atgaatttct gccgattcag
     7621 gcgcttcgta aacggaatct attcccgtaa agttgcgcag ttcaccggca cgcgctttct
     7681 tatataaacc ttttggatcg cgggcttcgc aaatcgccag cggcgtatcg acaaacactt
     7741 cgataaagcg cccttctcct acgcgttcgc gaaccatctg gcgttcggcg cggtgtggcg
     7801 agataaatgc ggtcagcacc accagtccgg cttcaaccat caaattcgcc acttcaccga
     7861 cgcgacggat attctcttta cggtcggcat cgctaaaacc gagatcgctg cataatccgt
     7921 ggcgaacgtt gtcgccatcc agcagatacg tactgacgcc aagtttatgt aacgcctcct
     7981 ccagcgcccc cgcgacggtt gatttaccgg aaccagagag gccggtaaac cacagcacaa
     8041 caccacgatg accgtggtgc tgttcgcgtt gttgcagagt tactgggtgg ctatgccaga
     8101 cgacgttttc gtcatgcaga gccattattt atctcccagt aaatcgcgcg caccccaatg
     8161 cgggaaatgg cggcgaacca gggcattcaa ttccagttcg aatgcactga attcagatgg
     8221 cgcagcagtt gcctggctaa ctggttcgtg caccataccg gcacctacgg tcacattact
     8281 caggcgatcg ataaaaatca gcccaccggt aaccgggttt tgctgataac gatctaacac
     8341 cagtggctcg tcaaaggtga gatctaccag gccgatgccg ttcagcggca ggttttcaac
     8401 ttcgcgttgg gtaaggttat tgatatcaac ctgataacga atgccatcaa cacgagcacg
     8461 cgtcttctta ccggcaattt tgatgtcgta actctggccc ggggaaagcg gctgttccgc
     8521 catccatacc acatccaccg acgcgctctg cacagccggt aacacttcgt ctgccgccag
     8581 cagcagatcg ccacggctga tgtcgatctc atccgtcagc accagggtga tcgcttctcc
     8641 ggcaaaggct tcttcgcgat cgccatcaaa ggtcacgatc cgcgcgacgt ttgattccac
     8701 accagagggc agcactttga cacgttgccc gacttccaca cgaccggatg ccagcgttcc
     8761 ggcgtagcca cgaaaatcga gattcggacg gttaacgtac tgcaccggga agcgcattgg
     8821 ctgagcatcc accactcgct ggatctccac ggtttccagc acttcgagca gtgtcggacc
     8881 gctgtaccac ggcatacttt cactttgcga agccacgttg tcgccttcca gtgcggagag
     8941 cggcacaaag cggatatcca gattacccgg cagctgcccg gcaaaggtca gataatcttc
     9001 acgaatacgg gtgaacgtct cttcactgta atccaccaga tccattttgt tgatcgccac
     9061 gaccagatgt ttgatcccca acagtgtgga gataaaactg tgacgacggg tttgatcgag
     9121 cacgccttta cgggcatcga tcagtaagat cgccagttca catgtcgatg cgccagtcgc
     9181 catattgcgg gtgtactgct cgtgccctgg ggtgtcggcg ataataaatt tacgcttctc
     9241 ggtagagaaa tagcggtaag ccacgtcaat ggtgatgccc tgttcgcgct cagcttgcag
     9301 gccgtccacc agcagtgcca gatccagctt ttcgccctgg gtgccgtgac gcttactgtc
     9361 gttatgcagc gatgagagct gatcttcgta gatttggcgg gtatcgtgta gcaggcgacc
     9421 aatcagggta cttttgccgt catcgacgct accacaggtc agaaaacgca gcaggctttt
     9481 atgttgttgc gcaatcatcc aggcttcgac gccgccttca ttggcgattt gttgtgcaag
     9541 tgcggtgttc atcttaaaaa tacccctgac gttttttcag ctccatagac cccgcctggt
     9601 cgcggtcaat tacgcggccc tgacgttcac tggtggtgga aaccagcatc tcttcgatga
     9661 tctccggcag tgtttgtgcg tttgactcca ccgcgccggt cagcggccag cagcccagcg
     9721 tacggaaacg caccatccgt tttttaatca cttcgcccgg ttgcaggtcg atacggttgt
     9781 catcaatcat catcaacata ccgtcgcgtt ccagaaccgg acgttccgca gcgagataca
     9841 gtggaacaat gtcaatattt tccagccaga tgtattgcca gatatcctgc tcggtccagt
     9901 tagagagcgg gaagacgcgg atgctttcgc ctttgttaat ttgcccgttg tagttgtgcc
     9961 acagctccgg gcgctgattt ttcggatccc agcgatggaa gcggtcacgg aaagagtaga
    10021 ttcgctcttt agcgcgggat ttctcttcgt cgcggcgcgc accaccgaag gcggcatcaa
    10081 aaccgtattt gttcagcgcc tgtttcaggc cttcagtttt cataatatcg gtatgtttcg
    10141 cgctgccatg aacgaatgga ttaatcccca tcgctacgcc ttccgggttt ttatgcacca
    10201 gcagctcgca gccgtaggct ttagccgtac gatcgcggaa ctcatacatc tcgcggaatt
    10261 tccagccggt atcgacatgc agcaacggga aaggcagcgt acctggataa aacgccttgc
    10321 gcgccagatg cagcatgacg ctggaatctt taccgataga gtagagcatc accggatttg
    10381 agaattctgc cgccacctcg cgaataatgt ggatgctttc cgcctccagt tgccgcaggt
    10441 gggtaagtcg tttttgatcc ataaccgttc ctttgcaata ccgctatttt cttgccatca
    10501 gatgtttcga ctatagggag cgtaagagaa cgaatgaaat taccaattag aatgagtagt
    10561 tccttaacgg aataacgatt tggcaaagct aatatcaaaa agtgcttaag gcaccggatt
    10621 tcgggcgttt aggaagattt gaaattgttt tagcgcagcg gcagtttcat actatggcgg
    10681 taaaaaaatt tgcatggtat ttaaggactc actatgtttt ccgcattgcg ccaccgtacc
    10741 gctgccctgg cgctcggcgt atgctttatt ctccccgtac acgcctcgtc acctaaacct
    10801 ggcgattttg ctaataccca ggcacgacat attgctactt tctttccggg acgcatgacc
    10861 ggaactcctg cagaaatgtt atctgccgat tatattcgcc aacagtttca gcaaatgggt
    10921 tatcgcagtg atattcggac atttaatagt cggtatattt ataccgcccg cgataatcgc
    10981 aagagctggc ataacgtgac gggaagtacg gtgattgccg ctcatgaagg caaaacgccg
    11041 cagcagatca tcattatggc gcatctggat acttacgccc cgctgagcga tgctgacgcc
    11101 gatgccaatc tcggcgggct gacgttacaa ggaatggatg ataacgccgc aggtttaggt
    11161 gtcatgctgg aactggccga gcgcctgaaa aatacgccta ccgagtatgg tattcgattt
    11221 gtggcgacca gcggcgaaga ggaaggaaaa ttaggcgctg agaatttact caagcggatg
    11281 agtgacaccg aaaagaaaaa tacgctgctg gtgattaatc tcgataactt aattgttggc
    11341 gataaattgt atttcaacag cggtgtaaaa acccctgaag cagtaaggaa attaacgcgc
    11401 gaccgggcgc tggcaattgc ccgcagccat ggaattgccg ccaccacaaa tccaggtttg
    11461 aataaaaact atccgaaagg caccgggtgt tgtaatgacg cagaaatatt cgacaaagcc
    11521 ggtattgctg tactttcggt ggaagcgact aactggaatc ttggcaataa ggatggttat
    11581 cagcaacgag caaaaacagc cgcattccct gcgggaaata gctggcatga cgtaagactg
    11641 gataatcaac agcatattga taaggcactt cctggaagaa tagaacgtcg ctgccgtgat
    11701 gttatgcgga taatgctacc gttggtgaag gagctggcga aggcatctta atgggtttga
    11761 aatgattact cccttgcctg caggaaatca atgccaccag aaacagagta taaaaaaggg
    11821 gtgcaggagg ccaaaccatc acaattcagt acgcggggtt ttaccccgcg tggtggctac
    11881 ttcttcgatt cgtaagcgag aactgcctta aactccatac cacgttctct gatactcaac
    11941 tcacacaacg agtcgcctac catcaacgtg aatcccagta ctgtacaaca cagtacgatg
    12001 attgccacaa gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca
    12061 tccgaattgc tacgttcgaa cgcgagcctc aggttgatag aaatatcgcc tggggctttt
    12121 gtccatctgg aaccttgcga atgcttaacg ccagacagcc tcaagcaccc aatgccattc
    12181 tatacctgtt aattttccct gcgatgttca cctttaaatg ccggaatgct gctcgcaaaa
    12241 aataaaaacg cctccatcat ttctgacaga ggcgtttaat ttatccggca atatttaccc
    12301 ttcgtgtaat ccacattccc tttttaagcc aaagaaacgc gtttcttctt ccgccatgcc
    12361 gggttcccat ttacgggttg tatgggtatc gcccaccgat aaatatcctt catcccataa
    12421 tgggtgatat ttcaggccat gtttttgcag gtactgataa atagttcggt tatcccagtc
    12481 gataatcggc agcactttaa atacgccacg ctgaattgcc agcaccggta aattggcgcg
    12541 gctgccggat tgttcacggc gcaggccagc aaaccaggtt tgcgcattca gttctttcag
    12601 cgcccggttc atcggttcga ctttgttgat gtcattgtac ttttcaatgc cttcaacgcc
    12661 ctgctcccac agtttgccgt agcgtgcttc ctgccaggcg gcgctttcgg tagcacgata
    12721 cactttcagg ttgagcttga gtttgtccgt taactcgtca ataaagcggt aggtttccgg
    12781 gaacagatag ccggtatcgg tgaggatcac tggaatatcc gggcgaatct gattcaccag
    12841 atgcaggctc accgccgcct gaatgccaaa gctggaagaa agtacatatt caccgggcag
    12901 attatccagc gcccaggcta cgcggccttc agcgtccagt ttttccagtt gggcgttagt
    12961 ttccgccagc gccagaatcc ggtctacctt tggcagttcg ttcagggcgt ttagatcgag
    13021 tttggacata agttcctcac tgtttgcctt gcctgatgca acgctcacac gtcttatcag
    13081 gcctacaaga ccgggctgat ggttaatccc acaaatcgcg cgccggatcg agcaccgggc
    13141 gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg
    13201 cccagcgccc tatcagttca tcaagcgacg ccaggatttc cggctcggtg atgttttctt
    13261 tatacatccg tgggatacgt gtcccaatgc ggttgccgcc aagatgcagg ttgtagcgac
    13321 ccggcgcttt ccccaccaga cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc
    13381 agcctgttac acgcatcacg atatgctcat cgctgacacc atgtttcgcc attaaattat
    13441 cgatgttgtc gataaaagac ggcaggaaac gctccgcttc cgccatcgcc agcgggcaag
    13501 tcgggaatga cacgcaggcc attgagtttt cacgctgcgg cgtgacggca ttcattaacc
    13561 cgctttcttt ggcgatcttc tcgatcttcg ctttctcgct ttccggcacg ccggcgatga
    13621 tcagattctg gttcgccgta atgcggaaat cacctttgtg gatcttcgcg atctccagca
    13681 agccggtttt cagcgggcgc cccggataat caagaattcg accattttcg ataaacagcg
    13741 tcaggtgcca gttatcatca atgcccttaa cccagccaat acgatcgccg cgtccggtaa
    13801 actcatacgg gcgaatcggc tcgaatttga tccccgcgcg acgttcaact tccgctttaa
    13861 acgtctcaac ccctacgcgc tccagcgtgt atttggtttt ggcattttta cgatcggttc
    13921 ggttacccca gtcacgctga gttgtcacga cggcttccgc caccgccagc gtatgctcca
    13981 gcggcagata gccaaactca ctcgccgtgc gggcgtaggt tttcttgttg ccgtgttcga
    14041 tggaaagccc accgcccacc agcaggttaa agcccaccag cttgccgttt tcggcgatcg
    14101 ccacgaagtt catgtcgttg gcgtgcagat cgatatcgtt ctgcggcggg atcactaccg
    14161 tggttttgaa cttacgcggc aggtaggtct gaccaaggat cggctcttca tcagtagtgg
    14221 cgactttttc ctgatcgagc cagatctccg catacgcgcg ggtacgaggc agcagatgtt
    14281 cagaaatttt cttcgcccat tcatatgctt ccgcatgcag ctgcgactcg taagggttcg
    14341 aggtgcagag tacgttacgg ttcatgtcgt tggcggtcgc cagcgcatca agaccgaccg
    14401 agtgcagcat ctggtgcacc ggtttgacgt tctttttcag aatgccgtgg aactgaaacg
    14461 tctggcggtt ggtcaggcga atgctgccat agatggtgtt ttcaccggca aatttgtcga
    14521 tcgcctgcca ctgtttagtg gtaatcaccc cacccggcag acgacagcga agcagcatcg
    14581 cgtggcgcgg ctccagcttc tgttcagcac gttcggcgcg aatgtcgcgg tcatcctgct
    14641 gatacatgcc gtggaagcgg atcagcagga agttatcgcc cttaaagccg ccggtcagac
    14701 cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct
    14761 cggcgtctgt cagttttcct tcgaccacta aaggccctgg atgtttttcg ctcattagta
    14821 gacatctcgc tgataacggc gctctacgcg cagctcactt aaaaattcat ccgccgcttc
    14881 ggtgtccatg ccaccaaatt cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc
    14941 catgcgatta gcgtcgccgc agacataaat gtgggcaccg tcattgatcc agcgccacag
    15001 ctctgcgccc tgttcgcgca gtttgtcttg tacgtaaatt ttttcttttt gatcgcgcga
    15061 ccaggcgaga tcgatgcgcg tcagcacgcc ttctttgacg taacgctgcc attccacctg
    15121 gtagaggaaa tcttccgtaa agtgtgggtt gccaaagaat agccagtttt tacccggcgc
    15181 ttcgtcggcg gcacgctgct gcataaaggc acggaacggc gcgatgccgg tgccagggcc
    15241 aatcatgatc accggggttt ccggattggt tggcaggcgg aagttgtcgt tatgttcgat
    15301 aaatacgcgg acttcaccct cttcttccac acgatccgcg aggaagctgg aggcaccacc
    15361 ggcacgggcg cggccttcca cgtcgtaacg caccacacca acggtaacgt gcacttcgtt
    15421 ctcgacttcc gcctgcgagg aggcgatgga atacaggcgc ggggtcagtg ggcgcagcag
    15481 attaatcagc gcttcggcat ccagttgcgc cggagagaaa cgcaccatgt cgacaatcgg
    15541 cgtcgtcgcg gcgtaatgct gtaacttcgc tttatcgccc accagcggca gcagcgtttc
    15601 gctgcgggta agcgtggcat agttctcaac aatgttggcg gtgttgacgg tcagttcgaa
    15661 gtgccactgt agcgcttcgt tcagaggcaa cgttttgccc tcgacggtga caggttcatc
    15721 gcctttcagc cacagcagtt cgacaagttc tttcaccagt gccggatcgt tttgatacca
    15781 gacgcccagc gcgtcacccg gctggtaacg caggtcagag tcacctaagt caatttcaat
    15841 gtgacgaacg tctttttcag agttacgccc ggtaattttc tggttaacag agaggctcgc
    15901 caccagcggc gcgtctttgc tatacgggct ggtgtggatt tcatttaccg tgccagtagc
    15961 gacggattgc gaaggtgccg cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc
    16021 gcgccactcg ctggcagcag cctggtattc aacgtcggca tcgacacggt cgagcaggcg
    16081 ttcaccgccc agttccgcca gcttactgtc aaaatctttc ccggactggc agaaaaattc
    16141 ataagagcta tcgccgaggc taaacacggc aaacgcggtg ttttccagct ttggcgcttt
    16201 tttggagaac aggaacttat gcagcgcgac ggcttcttcc ggcggttccc cttccccttg
    16261 cgttgacgtc accacgataa gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc
    16321 cgcgttcacc agcttaacgt tcagttttgc cgctaataaa tcatcgcgta atgcttcagc
    16381 aacccgacgc gcattgccag tttgcgaggc ggagataata gttatacccg gcatttctgc
    16441 ggcgggcgct ggcgtcgctg caagcgcagc aggctgctgg ttgagcacgc cccagaaata
    16501 gccagaaacc caggcaagct gggtaggagt taaatcggtc gtggccgcct gaaggcgtgc
    16561 cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg
    16621 ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag cgcccataaa acagaagaga
    16681 aggtaaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
    16741 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
    16801 gaaaaacaaa tgaatattgc ccatcaataa gataaatcag cgattttgcg caacaaagtc
    16861 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcatgt
    16921 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
    16981 ttgccgcacg tcccacaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
    17041 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
    17101 ctgaaagcgg cgtttaaacc aacttatgag cgcctcgatc accattatct caatgatatt
    17161 ccaggtctgg aaaacccaac cagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
    17221 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc aggttgtatc
    17281 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
    17341 gggtgttgtg ttctggatat ctggggcatg acatggaaga cgactgcgac attattatta
    17401 tcggtgccgg aattgctggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
    17461 ttttgttact ggaacgagcg gagattcccg gcagcaaaaa tctttccggc gggcgtttat
    17521 atacccatgc gctcgcggaa ctcctcccgc aatttcacct gaccgcgccc cttgaacgac
    17581 gcatcactca cgaaagtctt tctctgttaa cgccagatgg cgcaacgacg ttttccagct
    17641 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg attcgatccg tggctggttg
    17701 ccgaagccga aaaagaaggt gtcgaatgca tccccggtgc gacggtagat gcactgtatg
    17761 aagaaaacgg cagggtgtgt ggtgtcattt gtggtgacga tattcttcac gcccgttatg
    17821 tggtgctggc agaaggtgcc aacagcgtcc tggcggaacg ccacgggtta gtgactcgtc
    17881 ctgctggcga agcgatggca ttgggaatca aagaagtgct gacgctggaa ccatccgcta
    17941 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgttgttc agcggcggga
    18001 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
    18061 ggattgtttg cccgctctcc tcccttacgc aaagtagtgt tccggcaagc gagctgctga
    18121 ctcgctttaa aacgcatccg gcagtgcgcc cgcttatcaa aaacacggaa ttactggagt
    18181 atggtgcgca tctggtgcca gaaggtggcc tgcgcagtat gccggtgcaa tacgccggta
    18241 acggctggct gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
    18301 gcggcatgga tatggcgcta actggcgcgc aggcggcggc acaaacgctg ataagcgcct
    18361 gccaacaccg cgagccgcaa aatctgtttc cgctttatca tcacaacgta gagcgcagcc
    18421 tgctgtggga tgttctacag cgttatcagc atgttccggc gcttttgcaa cgtcctggct
    18481 ggtatcgggt gtggcctggg ttaatgcagg atatttcccg tgatttatgg gatcagggga
    18541 atacacctgt tccaccgctg cgccagttgc tctggcgtca tttacgtcgt cacggcctgt
    18601 ggcatctgac gggcgatgtt atcaggagtc tgcgatgtct gtagcccgta atctctggcg
    18661 cgctgctgat gcgccgcaca ttgttccgac tgactccgtt gagcgccaga cggcacaacg
    18721 gttgattaac gcctgtccag caggtctttt ttcgctcaca cctgaaggtg gcttacgtgt
    18781 tgattatcac ggctgcctgg agtgtggcac ctgccgtttg ctgtgcgacg aatcaacact
    18841 acaacagtgg cgctatccgc cttccggatt cggcatcacc taccgctttg gataaataat
    18901 aaggataatt tatgcccctc ttacacctgc tccgccagaa tccggtgatt gctgccgtta
    18961 aagataatgc cagcctgcaa ctggcaatcg actctgaatg ccagtttatt tccgtgttgt
    19021 acggcaatat ctgcaccatt agtaatatcg ttaaaaagat taagaacgcc gggaaatatg
    19081 cttttattca tgttgatttg ctggaaggcg cgtcaaataa agaagtggtg attcagtttt
    19141 tgaaactggt aaccgaggcg gacggaatta tcagtaccaa agcctcaatg ctgaaagcag
    19201 caagagcgga aggttttttc tgtattcatc gactgtttat tgttgattcg atttcgtttc
    19261 acaacattga taagcaagtt gcgcaatcga atccggattg tattgagatc ctgccaggct
    19321 gcatgcctaa agtgctgggc tgggtgacgg agaaaatccg ccaaccgctg attgctggcg
    19381 ggctggtgtg cgatgaagaa gatgcgcgta atgcgattaa cgcgggtgtt gtggcgcttt
    19441 ccaccacgaa tactggggtc tggacgttag cgaaaaaatt gctttgacgg gataatcgta
    19501 acaagttgaa tttggtgtga tttgtaggct gcacgccaca tccgacattc agcgcctgat
    19561 gcgacgcttg acgcgtctta tcaggcctac aagtccccag cccagaacca tcggcaggat
    19621 catccggcag cacgttattg gcaatctgcg tgaatgtttg tcaccagtgc ctccagcacc
    19681 actttccagt catcaactac gccaacatct gcctgtgaaa ataccgctgc actggcgtca
    19741 tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc tgctgcgccg
    19801 gaagcgccaa caacaataca cacctcaggt gccagcagat gtccggaaat accaatcact
    19861 ttttcggcat caacgccacc gttcatcact cgtgcccgac tgtagcccac ttcggctccc
    19921 atcttttctg ccagcatagc gatttcctgg ttatctgcct ctccgccctg ccccaccacc
    19981 agcacccgtc tggcttcagc taatgggtcg cggataacat ttttcagatt ttctatgcta
    20041 accagccagt cgggaagcac acctgggaca atgttcagtt gctgcatccc agagggcaac
    20101 gtggcgtttt tatctgctcc cgcctgacgt gccagcgaaa ggcatagcgg gcgtttgtct
    20161 gtttgcagcg tcgccgtcaa cgcatttccc cagtgggatt tgcgcacgct tacggttgga
    20221 atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc cagccgggtc
    20281 gacagttcat cgccaaacgc gcccggagga aaaaggacaa cgtccgcagg cgttcgttgc
    20341 cactgttcca ccagtgcatc cagcacctgt tccgcaacca tcggctgtgg ttctatttgc
    20401 caatgtgcca gggtacaacc gctgaaatcc tgcgccgcca gccagctcgc tattgcggca
    20461 tggtcctgat ttattgtcac aattacgata ttcatggctg catcctttgg cgtaaatagt
    20521 cctgccatag cttttgcgct ttttctgcta ccgtttgccc accaatcagc gtcgctccgc
    20581 gtcgttgctc tgctcttgcc agttgcagac actgcatcgc tggcaattcg acagcaacag
    20641 ttttacggat aattgctgct ttccccgcag ccattcgctg gcgcattccc ggaactggca
    20701 aggccacttc accgcactga cgcacggcaa tcaccgcagg caagcgaacc cggcaacagc
    20761 gcaccccatg ttcagtacgc tgttcgaggg tgataaacgg tgcgtcgaga gtaaagcgct
    20821 ccacctgggt gaagcagggc cagaccagca tttccgccag caaaaatggc gtttgcccat
    20881 tctgcccttc gctgctttgg cagccggtaa ttatcagatc cagcggattc tgacgctgcc
    20941 attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc gccgtctcca
    21001 gcaatacagc ttcctcaaac ccaagagcca tgagatagcg cagccagtgc aacgcccgtt
    21061 catcccccat gctcaacgcg gttaaagaca tcggcgtacc gttttttctc tgcgccaaca
    21121 gcagcgcagc tgcggcctgt tcatcagcac cgagtaaact tcgcagaagc gaaacatccg
    21181 gtccgctatt accctgagcc gccgcctgcc actctttttc cgccagcatt ccggcatccg
    21241 gttcggcttt aaacgctaac agaatgttca ttcgcacctc ctacatttcc cgccgccacc
    21301 agcgggagtg ctttggtttc tggtgcccat agccaggtca caacaaaacc aaccaacaac
    21361 actgccgcca ggagtaataa ggtggcttgc attccccact gcgccagcac ccacggcagc
    21421 aggccagtac ttaccgccgc ccccaaccga ctcatggcgg tggcaaaacc gacgcccagc
    21481 gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc ccaccagatt actgactgcc
    21541 gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa
    21601 caggccatca ctaccagcgt tgccgccagc agcaaaaaac ttcccagcaa aaacttgcga
    21661 tgtgccagca ggtgcgtcag aactaatccc agcagcgcgc ccacaattaa caacgcatta
    21721 agcatcagac tggcagtcag cgcatcttcc agtccaatag tctgggcgat agttggcagc
    21781 caggtataaa tcacaaacca tgggattacg aggcagacaa agaagacgct gttaaacgct
    21841 gtgcgccgcc agtagcgcga agagaacaag gttttgatgt gtttatgggt cgccgttgcc
    21901 acttcatcgc ccagtaaaac atgcgggcca aaatagcgat gaacgatagc gtgagcttct
    21961 gcaaaacgcc cctggcgcag tagccagcgt ggcgattccg gcgtgcccca acgtagcagc
    22021 gtaatcagca aggctggtaa cgccgctgat gccagcagcc agcgccaggc ctccgggctt
    22081 tcggaaataa agtgatgtcc ggcaatactt gccagcacat agcctacggt ccacaccaca
    22141 ctgaatgcgc ccagcaaaat accgcgatgg cggcgcgggg aaaattcagc cagcaaggtg
    22201 tgaccgactg agtaatcgcc tcccagacca atgccgatca aaatgcgtaa tccaataaga
    22261 tgctctggcg tggtggcaaa aaattgcaag aacgaagcga gcgtaatcag cataaaactg
    22321 aaggtgaaga ttttttgccg cccgatatgg tcggagatcc accccaaaac caggctgcca
    22381 agaaacaaac caagtaacgc cgagccgccg atcattcccg ccataaacgg cgtcagttgc
    22441 atggcgggcg taagctgaat aatggcataa ccaatgacgc cgagaacata accgtcggtc
    22501 aggtgtgcgc cgaaagtgag cgcagcaatt cgacagtgaa aacggttaag cggtaaatca
    22561 tccattcgca ccggtgaagt gttcatcttt accccttcat tgtccggtaa taagaacggc
    22621 ttctcgcctg agaagccgaa aaggcaacgc accgtcatcg cggaacgctg cctgacacat
    22681 tatttttcaa tcggatagat agtacccgtg ttcataatgc cattaggatc gaactgcttt
    22741 ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca gtttgctcca gtgaacgcga
    22801 tgtttaccga taccgtggtg atgcaccatc gaaccgccga ggcgaatggt ttcttcgcag
    22861 atgattttgt tgagcggatt gtggtacttg tcgatttcct cttccggctt acagtcaacg
    22921 acgttgtagt cgtagacgaa gtacatgttg gtgccgttct gatagctatg agaggagtga
    22981 ccgcccagca tggtgatgtc gtcggcgtgc gggaactcgg tacgaatacg gttaataacg
    23041 ctttcgtaga tttcgtgaat gcagctccag cagccggaca cttcagtggt aaagcccatg
    23101 ttgccggttt tgaggatctg cacacgttcg gcagccactt tatccggtcc ccagttcagg
    23161 ttgttaaacc aggtttcgat cagcttgctg tccacgcgct ggcactgcgg gtagcgcgcc
    23221 acgatttcag caatcccttc acccgtcgcc ttcgcaatgc gagggttacc ttcagccata
    23281 aagatcagca cgcatttgcc gtcggcaaaa tgggtgaagt gttgggtgcc atcttcagca
    23341 tcatacaaac gagcaatcga cggacgatac ccttccacca tgatttcacg caggatgttg
    23401 aagccggttt tcatatcttc caggatatag ccgtagaaga gattgttttc cggcgtaaat
    23461 ttgaagattt tcactgtgac ttcagtgata tagcacagtg caccttcgtt gccgatgatg
    23521 atatgacgaa tgtccgggcc agccgcgcga cgtggcactt ttttaatgcg tgtgacggtg
    23581 ccatctgcca gcaccgcttc cagaccaacg accatatctt cgattgcgcc gtagagtgtg
    23641 gagaactgcc cgatactgcg ggtcgccacc aagccgccca tctgcgccag cggctttgac
    23701 tgcggagaat gccccgtggt gtaacctttt tcgcgcaatg cgttttccag cacctccagc
    23761 ggaacgccac attgcgccgt ggcctgcata ttctcaatat caatattaat gatttgattc
    23821 atggcggagc cgtcgagcac caccgagttt tccacaacgg tttccagtcc accttcggtg
    23881 gcggaagcac cggtacgtgg cacaccgtta attttgtgcg cattcataaa attcagcaca
    23941 cgggatactt gttctgtgga accgagtttt acgaccgctg ccggaatcgg caaagtataa
    24001 attccatgaa tatccggaaa tttacgaaaa cggtcaatac tgtttttctt taataccgtt
    24061 tcatcggtaa taacgcgatc tgcaccaaca atttccttta gctggtcgac aatcgctacg
    24121 cgagataaag acataataat tccttcctga taataaaaat ggaatagtta tta
//
LOCUS       DACADA010000066        24090 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.067, whole genome shotgun sequence.
ACCESSION   DACADA010000066 DACADA010000000
VERSION     DACADA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.067"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            213..2456
                     /gene="pdeF"
                     /locus_tag="ISB17_002507"
     CDS             213..2456
                     /gene="pdeF"
                     /locus_tag="ISB17_002507"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAP1980431.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2495..4036)
                     /gene="ppx"
                     /locus_tag="ISB17_002508"
     CDS             complement(2495..4036)
                     /gene="ppx"
                     /locus_tag="ISB17_002508"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAP1980432.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4041..6107)
                     /gene="ppk1"
                     /locus_tag="ISB17_002509"
     CDS             complement(4041..6107)
                     /gene="ppk1"
                     /locus_tag="ISB17_002509"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAP1980433.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6279..6917)
                     /gene="purN"
                     /locus_tag="ISB17_002510"
     CDS             complement(6279..6917)
                     /gene="purN"
                     /locus_tag="ISB17_002510"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP1980434.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6917..7954)
                     /gene="purM"
                     /locus_tag="ISB17_002511"
     CDS             complement(6917..7954)
                     /gene="purM"
                     /locus_tag="ISB17_002511"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP1980435.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8279..8905
                     /gene="upp"
                     /locus_tag="ISB17_002512"
     CDS             8279..8905
                     /gene="upp"
                     /locus_tag="ISB17_002512"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP1980436.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8991..10280
                     /gene="uraA"
                     /locus_tag="ISB17_002513"
     CDS             8991..10280
                     /gene="uraA"
                     /locus_tag="ISB17_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP1980437.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10330..11076
                     /gene="hda"
                     /locus_tag="ISB17_002514"
     CDS             10330..11076
                     /gene="hda"
                     /locus_tag="ISB17_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAP1980438.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11214..11573)
                     /gene="arsC"
                     /locus_tag="ISB17_002515"
     CDS             complement(11214..11573)
                     /gene="arsC"
                     /locus_tag="ISB17_002515"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAP1980439.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11594..13057)
                     /gene="bepA"
                     /locus_tag="ISB17_002516"
     CDS             complement(11594..13057)
                     /gene="bepA"
                     /locus_tag="ISB17_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAP1980440.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13270..14331
                     /locus_tag="ISB17_002517"
     CDS             13270..14331
                     /locus_tag="ISB17_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP1980441.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14429..14899)
                     /gene="bcp"
                     /locus_tag="ISB17_002518"
     CDS             complement(14429..14899)
                     /gene="bcp"
                     /locus_tag="ISB17_002518"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAP1980442.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14899..15471)
                     /locus_tag="ISB17_002519"
     CDS             complement(14899..15471)
                     /locus_tag="ISB17_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAP1980443.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15617..16495
                     /gene="dapA"
                     /locus_tag="ISB17_002520"
     CDS             15617..16495
                     /gene="dapA"
                     /locus_tag="ISB17_002520"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP1980444.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16512..17546
                     /gene="bamC"
                     /locus_tag="ISB17_002521"
     CDS             16512..17546
                     /gene="bamC"
                     /locus_tag="ISB17_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAP1980445.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17759..18472
                     /gene="purC"
                     /locus_tag="ISB17_002522"
     CDS             17759..18472
                     /gene="purC"
                     /locus_tag="ISB17_002522"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP1980446.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18640..19503
                     /locus_tag="ISB17_002523"
     CDS             18640..19503
                     /locus_tag="ISB17_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAP1980447.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19518..21533
                     /locus_tag="ISB17_002524"
     CDS             19518..21533
                     /locus_tag="ISB17_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAP1980448.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21607..22305
                     /gene="ypfH"
                     /locus_tag="ISB17_002525"
     CDS             21607..22305
                     /gene="ypfH"
                     /locus_tag="ISB17_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1980449.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22386..22586)
                     /locus_tag="ISB17_002526"
     CDS             complement(22386..22586)
                     /locus_tag="ISB17_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAP1980450.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22614..23741)
                     /gene="dapE"
                     /locus_tag="ISB17_002527"
     CDS             complement(22614..23741)
                     /gene="dapE"
                     /locus_tag="ISB17_002527"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAP1980451.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23745..>24090)
                     /locus_tag="ISB17_002528"
     CDS             complement(23745..>24090)
                     /locus_tag="ISB17_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAP1980452.1"
                     /translation="YGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNGFINE
                     LGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPMLLGF
                     SESSYQQFFHEV"
BASE COUNT         5607 a         5998 c         6344 g         6141 t
ORIGIN      
        1 ttacaatgat gatgtaataa tgatgagtta acatgataaa cgtaataatt cattaatcag
       61 ggttattttg tttgatatat atcaattgga actttcatat gacacttaga atcattgctc
      121 tctatagtga tgaataatca tcattcgaag tcaggtggga tgcctgtctg aatacacctc
      181 cttcaggatg tgggggattc tgctgagcat ctatgaaact gaatgcaact tatataaaaa
      241 tacgtgataa atggtggggg cttccgctgt tcctgccttc tttaatcttg cccattttcg
      301 cccacattaa tactttcgcg catatttctt ccggtgaagt ttttctcttt tatctgccac
      361 tggcactgat gatcagcatg atgatgtttt tcagctgggc ggcattgcca gggatcgcct
      421 tagggatttt tgtccgcaaa tatgcagagc tgggttttta cgaaacgctc tcattaacgg
      481 ctaattttat tatcattatc attctctgtt ggggcggtta cagggttttt accccccggc
      541 gtaacaacgt ttcacatggt gatagccgtt taatttccca gcgactattc tggcagattg
      601 tgtttcctgc aacgctgttt ctgatacttt tccagtttgc tgcgtttgta gggttactgg
      661 cgagcagaga aaatctggtc ggcgtcatgc cctttaacct cgggacctta atcaattatc
      721 aggccttgct ggtgggtaat ctgattggtg tcccgctgtg ctactttatc attcgggtgg
      781 tgcgaaatcc gttttattta cgtagctatt attcgcaatt aaaacagcag gttgatgcca
      841 aagttaccaa aaaagagttc gcaatctggc tactggcatt aggtgcctta ttgttgctgt
      901 tatgcatgcc gttaaatgaa aaaagcacga tttttagcac caattacacc ttgtcattat
      961 tgctgcccct gatgatgtgg ggagcgatgc gctatggtta taagttgatt tcattgctct
     1021 gggcggtcgt gttgatgatc agcatccata gctatcaaaa ttacattccc atttatcctg
     1081 gctataccac gcagctgacc ataacctcct ccagttatct ggtattctct tttattgtca
     1141 attatatggc tgtactggca acccgtcagc gagctgtagt cagacgcatt cagcggcttg
     1201 cgtatgtgga cccggtggtt catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg
     1261 atgccccctg gtctgcgcta tgctatttac acatccctgg catggaaatg ctggttaaga
     1321 actatggcat catgctgcgg attcaataca agcaaaaact ttctcactgg ctgtcaccat
     1381 tgctggaacc gggtgaagat gtttatcagc tttcgggtaa cgatctcgcg ctgcggctga
     1441 atacagaatc gcaccaggag cgcattaccg cactggatag ccatctcaag caatttcgtt
     1501 tcttttggga tggaatgccg atgcaaccgc agattggcgt cagttactgc tatgtgcgct
     1561 cgccagtgaa tcatatctac ctgctgctgg gagagctaaa tacggtggcc gaactttcca
     1621 tcgtgaccaa cgtcccggaa aatatgcagc gtcgcggagc aatgtatttg caacgcgaat
     1681 tgaaagataa agtcgcgatg atgaatcgac tacagcaggc gctggaacac aaccattttt
     1741 tcctgatggc ccagccgatt accggtatgc gtggtgatgt ttaccatgaa attcttctgc
     1801 gcatgaaggg tgagaatgat gagctgatca gccccgatag cttcttaccg gtcgcgcacg
     1861 aatttggttt atcgtcgagt atcgacatgt gggtcattga gcatacgctg caatttatgg
     1921 ctgaaaacag agcgaagatg cccgctcacc gttttgctat taatctgtct ccaacctcgg
     1981 tatgtcaggc tcgttttcct gttgaagtca gtcagctgct ggctaaatat cagattgaag
     2041 cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc
     2101 aaatcacctt gcagcatctt caggaattag gctgccagat tgcgattgat gatttcggca
     2161 ccggctacgc cagctatgcg cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg
     2221 gcagttttat ccgcaatatt gtgtcaaata gtctggatta tcagatagtg gcgtcgattt
     2281 gccacctggc gcgaatgaag aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga
     2341 tccgcgaggc ggtgctctct ttggggatcg attatatgca gggttatctt attggtaagc
     2401 cgcaaccgtt aattgatacg ctgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa
     2461 tgcgggccga catttctcgt cggcccgcaa agcattaagc ggcgatttct ggtgtacttt
     2521 cttcttcaat tttcaaccgc cagccagcca cgccttccca gtattcttgc tccttttcca
     2581 gatcaagcag taccagcgca ttctgactaa accagtcatg cgggaaacgc agtgtccagt
     2641 gactgtcatc agtaatcagc gtcaatgttg gcggtgtggt ggttgcctga cgttgattat
     2701 tgagtaatac gccaaggcgc aatagctgaa tcagtggcag gaactgtttc ttcttaaaca
     2761 aggtaaagcg cggcagatcg tcgagtttaa tcgctttacg gtgatagcgc accagtgtcg
     2821 ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc gctgttttgc agaatataag
     2881 cggagtggcg atgcaaaccg ctgtggttga tattcaaccc gacctcatgc agcatggcgg
     2941 cccatcgcag tagcgcctcc agttgtggat gcgccagctt cggttgctgt tcccgccact
     3001 gttcgtacat ttgcatagtg gtatccagca cccgtcgggc ctgttcgctg tcgatgtgat
     3061 actggttggc gaggctgctg gcagtgcgac tacgcacatc ctgatgacgg aaacgtcctt
     3121 ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga aaggcgcagt tcacggatgg
     3181 ctaaagcatc aaacacaccg cataaaatcg ccagtcccgg aacgaagact gttttccgtt
     3241 cttcggaaag acccggtaaa ctcagcgatg cgaaattacg gtgacgtaaa acttctttta
     3301 ccagtttttc cagacgttcc ggggtaatta tcccgtcttt ctcgcccatt tccattaaca
     3361 cttcatgggc ggcttttatg gtaccggaag cgcccattgc cacgttccag ccctgaattc
     3421 gaaattgcca ggttaacgtt tccagttttt gtgctgccgc catgcgagcg cgctgaaaat
     3481 tctctttatt gatgaccccg ccagggaaat aaagctgggc aaagctgaca caacccatcc
     3541 ggcggctttc aacgagaatc ggttcgaaat tttcaccaat caccagttct gtagaaccgc
     3601 cgccaatatc aataaccagt ttgcgacctt tttccggttg ggtatgttcc acgcccataa
     3661 aaatcagacg ggcttcttca ttaccggaaa taatttcgat cgggtaggga atgacttttt
     3721 ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg cagcgtatgg gtaccaacta
     3781 tacagacgct ggcaggggaa aacccttgta gcctttcggc aaatagcgac agacagttca
     3841 aaccgcgcgt cattgcctct tcactcaaca tattatctgg ccccaggccg tccgccagat
     3901 gcacccgctg tttcaggcgg ccaataatct gcatggcacc atctaccaca cgggctatga
     3961 ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa ctcctgcgga cgaggggatt
     4021 tatcgtgtat tggcataggg ttattcagat tgttcgagtg atttgatgta gtcgtaaatc
     4081 gccaactgcg cccgtacttt gcggcgattg ccgcggggaa cgtagcgatt actgagttct
     4141 ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc
     4201 cgctgcttca ggcgcggatc gagcagcggc gttgccactt caatacgata atcaatattg
     4261 cgcgtcatcc agtcggcgga agaaaggtag acctttttat cgccgccatt ttcaaaaata
     4321 taaacccggt catgttcaag gtaacggtca acaatactga tggcacgaat gttgtcgcta
     4381 atgccttcca gattggggat cagagaacac attccgcgca ccagcagatt aaccggtacg
     4441 ccggagctgg aggccgcata cagacgatca accagacctt tatcgacaag gttatttagc
     4501 ttcagggtga taccactggg cagcccttgc tgcgcgttgg cgatctcgcg gtccaccatt
     4561 tcatacaaca gacggcgaga gttttgcggc gacaccatta aataatcaaa tgtcaccgga
     4621 cggtatgggt tttcaataaa gttgaacacc cgccgtactt cgttagtgat gcgcgcatcg
     4681 gcggtcagca acgaatagtc agtataaaga cgcgcggttt tttcgttaaa gttcccggtc
     4741 ccgatgtgtg cgtaacgcac cacttcaccg ttttctttac gtgaaatcag gaacagtttg
     4801 gcgtgaattt tcagccccgg cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc
     4861 ttcgcccagt gaatgttggc ttcttcgtcg aaacgtgcct gtaactcaac caccacggtg
     4921 actttcttac cgttgtgtgc ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc
     4981 acgcggtaaa tgttaatttt aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc
     5041 agttccagta catgctcaaa ggtatgataa ggataataga gcagcacatc gcgctcacga
     5101 atggcatcaa aaccgttgcg gaactgggct ttatcaaacc aaatgtggcg taaacgtggc
     5161 agtggtttgt tcaccagatt ggctttgccg acattgggga aattaataaa gtctttaaaa
     5221 ttatgataac gaccgccggg gacgatggag tcgtagcggg aaatagtcag tttttcgcgt
     5281 aacacttcaa ccagcgcatt gggcatatcg cgctgataaa caaaacgcac cggctcagca
     5341 gttaaacgct gcttgagact ggaagacatc aactccatca ggctggcttc catctcatgc
     5401 actaaatcgt attcggcatc gcgggtcatc ttcattgaat aggcattcag cgcgtcataa
     5461 tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca gaatgttatc cagaagaatc
     5521 atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca caaagcgcgg cactttatct
     5581 gatgggatct ccagcagcgc gtaacggatg gtatcgccac ggataatttc caccgccaga
     5641 taggtgtaat catctttcag gaactgcact aaatcagtgt caggattgat taaaatcggc
     5701 gtaatgtgct gacgcagata ctgcttaaaa taatgacgca gccagttttg ttgattgacg
     5761 gagagctggc gttcattaat caggaagatc tggttgcgcg ccatctctag caacaactcg
     5821 ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc gggactgaat tttgcccagt
     5881 aaatggcggg aatgagagtt ggaaccttgt tcttcgctaa taatgatgcg tcgcttcagt
     5941 tcagcgaagc ggactttata gaactcatca aggttattgg aatagatccc caggaaacgc
     6001 atcctttcaa tcagcgggtt agatttgtcc gccgcttcct gaagcacgcg ttcattgaac
     6061 gataaccaac tgagctcttt ttcgatgtat agcttttcct gacccattac cacttttact
     6121 ccgttttatt cacgggacac tgcctggata ttatggcgag caatattggc agatgtccaa
     6181 ctatgccaga aaagggggaa aaaaactgct ggagcttgcg atttacaggt gaattgaaaa
     6241 acgccagcgg cagagctggc gctttaacta cgggggcatt actcgtcggc agcgtagccc
     6301 tgcggcggca gacgttgacc atccagccac gcggcgtttt cgtgcatttt cagacgacca
     6361 tcggcaaacc agctaatcac cagtggataa atagcgtgtt cctgggtttg cacgcgggcg
     6421 gtgatgtcat cttccgtatc accagcaaat accgggactt tcgcctgtaa aataaccggg
     6481 ccaccgtcca gttcatcggt gacgaaatgc accgatgtac cgtgctcttc atcgccattt
     6541 tccagcgcct gacgatgggt gtgtaatccg ggatatttcg gcagcagaga agggtgaatg
     6601 ttcagcaaac gcccagcata gtgggagaca aacgccgggc tgagaatgcg cataaaacca
     6661 gccagcacga ccacatcggg tgcgtacatg tcgatttcat gaatcaactc ccggtcatag
     6721 gcttcacgac tgtcaaacgc gctggtgatg agcgtatgcg ttgcaatacc cgcctggcgg
     6781 gcgcgttcaa ggccgaacgc gtcggcctta ttgctgaaaa ctgcccgtac ggtgccttta
     6841 attttgttgg ttttgcaggc gtcaataatt gcctgcaaat tacttccgtt gccggaaata
     6901 agcaccacaa tattcattat tcgataacca cgcgttgttc ggaatcagag gctttgatga
     6961 taccgatttt ccacgcgttt tcaccgttgg cattgagcag ggcgagggct ttgtccactt
     7021 ccggagcagg cagggcaata atcataccga cgccgcagtt gaaggtgcga tacatttcat
     7081 ggcgctcaac gttacctgcc gtttgcagcc agttgaacac ttccggccac tgccaggaag
     7141 attcatcaat cactgcctgg gtattatctg gcaatacgcg cggaatgttt tcccagaagc
     7201 cgccgccggt caggtgcgca atggcatgca catcgacctt ttcaatcaat ttcagcactg
     7261 acttcacgta aatgcgggtc ggtgccagca gatgatcggc taatggctta ccatcaagtt
     7321 cggtggtttg cggatcacaa ccgctgactt caagaatttt gcgcaccagc gaatagccgt
     7381 tcgagtgcgg gccgctggag ccgagtgcaa tcagcacatc gccgtcgctg actttagatc
     7441 cgtcgatgat ttctgatttt tctaccacgc caacgcagaa accagcgaca tcgtaatctt
     7501 caccgtgata catccccggc atttccgccg tttcgccacc caccagtgaa cagcccgatt
     7561 gcagacaacc ttccgcaatg ccgctgatca ccgctgaagc ggtatcaaca tccagttttc
     7621 cggttgcgta atagtcgagg aaaaacagcg gttccgcacc ttgcaccacc aggtcattaa
     7681 cgcacatggc gaccagatca ataccaatgg tgtcgtgacg ttttaagtcc atcgccagac
     7741 gcagcttggt acctacgccg tcagtgccgg aaaccagcac aggttcacga tatttttgcg
     7801 gcaatgcaca cagcgcaccg aagccgccca gaccgcccat cacttccgga cgacgcgttt
     7861 tcttcactac gcctttgatt cttccaacca gagcattacc cgcgtcaata tcaacaccgg
     7921 catctttgta gctaagagag gttttatcgg tcactgcttg ggtccccacg cgttacttgc
     7981 ggtagaaaaa taaaattcgg cgcaattcta acagggaaag caaacgtttg cgagactgct
     8041 ttacacaacc tttttgcacg tcttttcccc aggcgcgcgg cgaaagaata cttgtaccag
     8101 ggcaaaggat cgttttcgga tggaataatc ttctttcata actatttgaa tataaaataa
     8161 ctttatctca aaccgttatc attttgacta aagtcaacga aaataatatt gccgccttga
     8221 agaaaggagg tataatccgt cgattttttt tgtggctgcc cctcaaagga gaaagagtat
     8281 gaagatcgtg gaagtcaaac acccactcgt caaacacaag ctgggactga tgcgtgagca
     8341 agatatcagc accaagcgct ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta
     8401 cgaagcgacc gccgacctcg aaacggaaaa agtaactatc gaaggctgga acggcccggt
     8461 agaaatcgac cagatcaaag gtaagaaaat taccgttgtg ccaattctgc gtgcgggtct
     8521 ggggatgatg gacggtgtgc tggaaaacgt tccgagcgcg cgcatcagcg tcgtcggtat
     8581 gtaccgtaat gaagaaacgc tggagccggt accgtacttc cagaaactgg tttctaacat
     8641 cgatgagcgt atggcgctga tcgttgaccc aatgctggca accggtggtt ccgttatcgc
     8701 gaccatcgac ctgctgaaaa aagcgggctg cagcagcatc aaagttctgg tgctggtagc
     8761 tgcaccagaa ggtatcgctg cgctggaaaa agcgcacccg gacgtcgaac tgtatactgc
     8821 atcgattgac cagggactga acgagcacgg atacattatt ccgggcctcg gcgatgccgg
     8881 tgacaaaatc tttggtacga aataaagaat aaaaataatt aaagccgact ttaagagtcg
     8941 gctttttttt gagtaaagcg cctataacac ataatacaga ggataatact atgacgcgcc
     9001 gtgctatcgg ggtgagtgaa agaccgccac ttttacagac aatcccgctt agtttgcaac
     9061 atttgttcgc catgtttggt gcaaccgtcc tggtgcccgt cttatttcat attaacccgg
     9121 cgactgtact gttatttaac ggtataggaa cactgctgta tctcttcatc tgtaaaggga
     9181 aaattccggc ttatcttggt tccagctttg cctttatttc accggtattg ttactgttgc
     9241 cgttggggta tgaagtcgcg ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg
     9301 tttcttttat cgtgaagaaa gcggggaccg gctggctgga cgtgctgttt ccacctgcgg
     9361 caatgggcgc aatcgttgcc gtcatcggtc tggagctggc gggcgtagct gccggtatgg
     9421 cgggtttact cccggctgaa gggcaaacgc cagactccaa aaccatcatc atctctatta
     9481 ccaccctggc ggtcacggtt ttaggttccg tgctgtttcg tggtttcctg gcaattatcc
     9541 cgattttaat tggcgtgctg gtggggtacg cgctctcttt cgcaatggga attgtcgata
     9601 ccacgccgat tattaatgct cactggtttg cgctgccgac cctctatacg ccgcgcttcg
     9661 agtggtttgc cattctgact attctgccag cggcgttagt ggttattgcc gaacacgtag
     9721 ggcacctggt agtaacggct aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc
     9781 accgttcgat gtttgctaac ggcttgtcga ccgtgatctc cggcttcttt ggctccacgc
     9841 caaacaccac ttacggagaa aacattggcg tgatggcgat cacccgtgtt tacagtacct
     9901 gggttatcgg cggtgcggcg attttcgcta tcctgctttc ctgcgtcggt aaactggctg
     9961 ctgctatcca gatgattcca ttgccggtga tgggcggcgt ttcgctgctg ctttacggtg
    10021 tcatcggtgc ttccggtatt cgtgtgttga tcgaatcgaa agtggattac aacaaagcac
    10081 agaacctgat cctgacttcc gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca
    10141 ttggggcggc ggagctgaaa ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt
    10201 tgatcttcaa actgattagt atgctgcgcc ctgaagaagt ggtgctggac gcagaagacg
    10261 cggatataac agacaaataa tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca
    10321 tgggattctg tggtaaactt ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac
    10381 acaccggcac agctctcttt gccactttat cttcctgacg acgaaacctt tgcaagtttc
    10441 tggccggggg ataactcctc tttactggcc gcgctgcaaa acgtgctgcg tcaggaacat
    10501 agcggttaca tctatctctg ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg
    10561 gcttgcgcgg aattgtcgca gcgtggcgat gcggtgggct atgtcccgct ggataaacgc
    10621 acctggtttg ttccggaagt gctcgacggt atggagcatt tgtcgctggt ctgtattgac
    10681 aacattgagt gtattgccgg cgatgagttg tgggagatgg cgattttcga tctctacaat
    10741 cgaattctgg aatcgggcaa aacacggttg ttgatcaccg gcgatcgtcc accgcggcag
    10801 ttgaatctgg gattaccgga tctcgcgtcg cgtctcgact gggggcagat ctacaaattg
    10861 cagccacttt ctgatgaaga taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt
    10921 tttgaactgc cggaagatgt ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc
    10981 acgttgttta tgacgctgga tcagctggat cgtgcgtcga ttaccgcgca acgcaagctg
    11041 accattcctt ttgtgaaaga aattctgaag ttgtagataa ggtgtttatt gtcggatgcg
    11101 gcgcgaacgc cttatccgac ctacgcgaag ttaatccata gtattcgtag gccggataag
    11161 acgcggcaag cgtcgcatcc ggcaatgtgc ttcaactagc gatggagcac atcttaaccc
    11221 acgatttcca gtacctgctc cggtggacga ccaatccgtg ctttgccatt cgcgaccaca
    11281 atcgggcgtt ccattagttt cggattatcc accatcgcct gaatcagcgc ctcttcgcta
    11341 agtgaactgt cagccaggtt cagttcttta taaagatcct ctttttggcg catcaattca
    11401 cgagcgctat tcatccccag cattttcagc aaatcacgca gcgttgccgc atcggcgggt
    11461 gtctcaaggt agagcaccac ttctggctcc acgccgtttt ctttcagcag gttcagcgtt
    11521 tcccggctct ttgaacagcg tgggttatgg tagattttta cctgtttggt cattgttctt
    11581 cctttaatgc gaattacatc ttggtataag gcttaaagcg ttcctgcagc tggcgcaact
    11641 ggtcgatgcg cgcatcgtaa cgcgcttgtt gcaggctgcc taatttcacc tgcgaactgg
    11701 cgctactcaa caaggaaata gcctgatcga gtcgcccggc gagcgcataa ccttctgctc
    11761 gcgcagccag ttcctgatcg cggttattta gtgcggcttc cgcctgtgcc agtaaatccc
    11821 agccgttgct gtcatcttta ttgttaaaag tgtagcgatt cagaatattg gccgcttcct
    11881 gtggttgccc cccttgcaga taagcgttcg ccaggttgag ctgcaacacc ggattggtgc
    11941 gcaaatcgcg ggcatttttc aggcgattga tagcgtcatt ggctttgttt tgcccaagat
    12001 cgatatcggt agccagatcg agataccatg cgttgccagg ttctgctgcc agtaacggtt
    12061 gcagcgtttt acgcgcttcg tcgtatttat tggcttccat cgcctgtaga gcacgaccat
    12121 attgcgccgc tcgttgctga cgaacgtttc ctttcgccca ttcatccagc aaatcactgg
    12181 tgagctggtt acgtccggaa ttatacatcc ccagtgtgcg cgctttcgcc agatagaaat
    12241 cttccgacga ctgcaccacc atcgggcgca tctgattagc acggttgcgg gcatctgcca
    12301 gacgactttc cggcaacggg tgagtcaata aaatttccgg cggacgcgag gagtaacgcg
    12361 cctgatcgag taatttttcg aggaaggtcg gcatcgcctg cggatcgaat cccgagcgtt
    12421 gcagcacctg aataccaatg cggtccgctt cctgttcatt ttgctgggtg aaactgatca
    12481 tcccctggcg cgttcccgcc agtgtaccgg tcagcgccgc catccccgcc tgcggactgg
    12541 ccatcgccag taaaatagaa cctaacgcgc cgacccaggt cagcggcgcg ctgcgctgct
    12601 gatcttccat cgctcgcgcc aggtgacgtt gggtgacgtg ggagatttcg tgcgccataa
    12661 ctgaagccag ttgactttcg ttatcggaat aacggaacag ggcagagtgc agcaccacgt
    12721 tgccgccaaa gaaagcaaag gcgttaattt cgtcgttgtt gatcagaaag aaatgaaacg
    12781 gtgtcttaac cgaattggca tgcgaaacca gacgcattcc tagcgaatta atatattgcg
    12841 ttaatagcgg gtcattaatt aacggcgcgc tgccgcgtaa ctggcggaca taatagtcgc
    12901 ccatctgcat ttcctgacca atggaaagtg tgcttcctgc ggaggttccc atatccggca
    12961 aggtgtctgc gctgtcggca aacgccgggg ctacctgacc aatggtcata gcagcaatga
    13021 gggttgcaac caggtttttt ttcaactgcc tgaacataac ctctatcctg tatttctgga
    13081 gatagtcatt tgaccgatac tgtgaaatat cgttcccacc gcgccgcctt tgagtgtaac
    13141 gacgaagatt agcccactca aataactttt cagtttcctg aatccgtcat aaaaagcgaa
    13201 gtcttttttg tgacatttcg atacaattgc ggatcgcaat cccgctcttg aggttcaggt
    13261 aaggtctgta tgctcgaaat gttgatgcaa tggtatcgcc gccgttttag cgacccggaa
    13321 gcgattgcct tgctggttat tttagtcgcc ggatttggca ttatcttttt ctttagtggc
    13381 ctgcttgctc cgttgctggt ggctattgtg ctggcctatt tgctggagtg gccaaccgtg
    13441 cgcctgcaat ctattggctg ttcccgccgc tgggcgacgt cgattgtatt ggtggttttc
    13501 gtcggtatat tgctactgat ggcgttcgtg gttctgccta ttgcctggca gcagggcatc
    13561 tacttaatcc gcgatatgcc ggggatgctc aataagcttt ctgactttgc cgccacgttg
    13621 ccgcgccgct atccggcgtt aatggatgcg ggcattattg atgcaatggc cgaaaatatg
    13681 cgcagtcgga tgctgaccat gggcgattcg gtggtgaaaa tttccctcgc ctcgttggtc
    13741 ggtttgctga ccattgccgt ctatctggtg ctggtgccat tgatggtctt cttcctgctg
    13801 aaagacaaag agcagatgct gaacgccgtt cgccgggtgc tgccgcgcaa ccgtggactg
    13861 gcaggacagg tgtggaagga gatgaatcaa caaatcacca actatatccg cggcaaagtg
    13921 ctggagatga tcgtggtagg gatcgccact tggctggggt tcttgctctt tgggctgaac
    13981 tattcgctgc tgctggcggt gctggtcggc ttctcggtac ttattccgta cattggcgca
    14041 tttgtggtga ccattccggt ggttggcgtg gcgctattcc agtttggtgc tggcacggaa
    14101 ttctggagct gcttcgcggt gtatctgatt attcaggcgc tggacggcaa cctgttagta
    14161 ccggtgttgt tctccgaagc ggttaacctg catcctctgg tgattatttt atcggtggtg
    14221 atcttcggtg gtttgtgggg attctggggc gtattcttcg ctattccatt ggcgacgctg
    14281 atcaaagccg tgattcacgc ctggcctgat gggcaaatcg cgcaagactg accttcagcg
    14341 aaaaatttga aacgagtgtt gcagggcagt gagaagattt gagatgtgct ggccgcaagc
    14401 gcagccagca cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa
    14461 cgtcgtggtg attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt
    14521 caatcaggaa gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttcac
    14581 cccagacgcc gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa
    14641 gcagctcttt ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca
    14701 catcaacgcc cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg
    14761 tacagccggg ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt
    14821 cggtcaaatt aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac
    14881 cggctttcag tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac
    14941 gacgttaata ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat
    15001 atttgctgcg tccgcagatg cggggctgtg ggcagttatc tgaatatgta actgcgcagc
    15061 ccgttcattt tcagcaggtt gcgtgcgcga caccagctcc gcaatgttca tatgatgcgc
    15121 gtcgaaaagt gctgtgaagc gttcaattaa atgcggggag tctgccacat cgacctgaac
    15181 ccagacagat gctggcattg gtggacgcgg acgcgccgtc gtgcgcttca tcacgattaa
    15241 aagatccagt tcggcacctt tcaacggtaa cgttgattca atcagggtga ttgcattcca
    15301 tgaaccggaa agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc
    15361 ttcaatatta cagccgcaac tactgacatg acgggtgatg gtgttcacta ttccagggcg
    15421 atcggcaccc aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat
    15481 tccttcaagg ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat acaacaatca
    15541 gaacggttct gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt
    15601 gcacagagga tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga
    15661 aaaaggtaat gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg
    15721 tacttcggcg atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga
    15781 acatgctgat gtggtgatga tgacgctgga gctggctgac gggcgcattc cggtgattgc
    15841 cgggaccggt gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag
    15901 cggtatcgtc ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt
    15961 gtatcagcat ttcaaagcca tcgctgaaca tactgacctg ccgcaaattc tgtataatgt
    16021 gccgtcccgt actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa
    16081 aaatattatc ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga
    16141 gctggtttca gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat
    16201 gcaattaggc ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc gtgatatggc
    16261 ccagatgtgc aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg
    16321 tctgatgcca ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc
    16381 atgtaaggaa ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac
    16441 cgacagtggt cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt
    16501 agggagattt gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt
    16561 cgcttgtttt attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg
    16621 gtgatgaagc ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga
    16681 ttttgccggt gacctccggc gattatgcaa tcccggtgac caacggtagt ggtgctgtcg
    16741 gtaaggcgct ggacattcgt ccaccagccc agccgttggc actggtttct ggcgcgcgta
    16801 cccagttcac gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt
    16861 ggccgcaggt ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg
    16921 ctggtcagac actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt
    16981 atcgtggtcg ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta
    17041 aactgctgaa cctggaacag gcgggcaaac cggttgcaga cgcggcgtcc atgcagcgtt
    17101 acagcacgga gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg
    17161 cgaacgctgc gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca
    17221 ccggtttacc aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag
    17281 ctgcgctgga aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg
    17341 ccgtaactta taagccgctg tctgacagcg aatggcacga actgggcgcg agcgatccag
    17401 gtctggcatc cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac
    17461 agttcatcga tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg
    17521 tcttccaggc tgcgtttagc aagtaaaaat acagggctgg agtcatccgg cccttttttc
    17581 tgatatgata cgcaaacgtg tgcgtcggca gaaaaacgcg attttagcgg taattcgcac
    17641 gaaatttgtt tgtcggacgt aggtcggata aggcgttcac gccgcatccg acaaaacatc
    17701 cggcacacca gacagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat
    17761 gcaaaagcaa gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc
    17821 ggacctgttg gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga
    17881 gcagtttgat cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact
    17941 ggctgaagcg ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt
    18001 gaaaaagctg gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct
    18061 ggtgaaacgt cttggaatcg aagaaggtat tgagctgaac ccgccgctgt tcgatctgtt
    18121 cctgaaaaac gacgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg
    18181 ctgggtgagc aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt
    18241 gctgaaaaaa ctgttcgatg atgctggtct gattctggtc gacttcaagc tggagtttgg
    18301 tctgtacaaa ggcgaagtgg ttctgggcga tgagttctcc ccggacggca gccgcctgtg
    18361 ggacaaagaa acgctggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct
    18421 gatcgaagcc tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg
    18481 ttcatcatct tgccgtgctg ctggcacggc aagacaaccg ctctcataag atgagtattg
    18541 aacgctcatt cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc
    18601 tacgatcatt tctataatga atataatgtt gaggtatcta tgcgttggca agggcgacgt
    18661 gaaagtgaca atgttgaaga caggcgcaat agctccggcg gtccgtcaat gggcggtccc
    18721 ggttttcgcc tgccaagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca
    18781 ggctactatg gcgttgattt aaccgggttg atgaccggac agccggtttc ccaacagcaa
    18841 tcaacgcggt caattagccc taatgaagac gaagccgcaa aattcacctc ggtgattctg
    18901 gcaaccacgg aagacacctg gggacagcaa ttcgagaaga tgggtaaaac ctatcagcaa
    18961 ccgaagctgg tcatgtaccg tggaatgacg cgtaccggct gcggggcggg ccagtccata
    19021 atggggccat tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat
    19081 gacatgaaag acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgctcat
    19141 gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa
    19201 caaaacgcga cgcaggcgga agtaaaccgc ttatctgtgc gtatggaact ccaggccgac
    19261 tgttttgccg gtgtctgggg gcatagtatg cagcagcaag gtgttctgga aaccggcgat
    19321 ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt
    19381 caggggcgag tagtaccaga cagtttcact catggcactt ctcagcaacg ctacagctgg
    19441 tttaaacgtg gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt
    19501 taacttccgg ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga
    19561 aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga agagcgttgg tgttttgatc
    19621 atgcgcttaa gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag
    19681 atgctgaaaa ccactgttct ccctcggcac tacaaacttt acttgggcgc gagttccggc
    19741 atgcggtatt cgacgcccgc cacggctttg atgccgctgc ctttgcggca cttagcggaa
    19801 cgttgaaagc gggaagctgg ctggtgttgt tactccctgt atgggatgag tgggaaaacc
    19861 aacctgatgc cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt
    19921 ttgtccagca tttcaaacgc gtacttacgg cgaataacga cgctatcctc tggcggcaaa
    19981 accagccgtt ctcgttggcg cattttactc cccgtactga ctggcacccc gctaccggcg
    20041 caccacaacc agaacaacag caactcttac agcagctact gaccatgccg ccgggtgtgg
    20101 cagcggtaac tgctgcgcgt gggcgcggta agtcggcgtt ggcagggcaa ctcatttctc
    20161 gtattgcggg cagtgcgatt gtcaccgctc ccgcaaaagc ggcaacggat gtactggcac
    20221 aatttgcggg cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc
    20281 aagccgactg gctggtggtc gatgaagccg cagctatacc tgcgccgttg ttgtatcaac
    20341 tggtatcgcg ttttcctcga acgttgttaa ctactacggt gcagggctac gaaggcaccg
    20401 gacgtggttt tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactac
    20461 aacagccgat ccgttgggcg cagggatgcc cgctggaaaa aatggttagt gaggcactgg
    20521 tgtttgacga tgaaaacttc acccatacac cacaaggcaa tatcgttatt tccgcatttg
    20581 aacagacgtt atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg
    20641 gtgcgcacta ccggacctcg ccgctggatt tacgccgcat gatggatgca ccaggtcaac
    20701 attttttaca ggcggctggc ggaaacgaga ttgccggagc gctgtggctg gtggatgagg
    20761 gcggattatc tcaagaactc agtcaggcgg tatgggcggg ttttcgtcgc ccgcggggta
    20821 atctggtggc ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg
    20881 gacggcgggt tagccggata gcagttcatc cgactcgtca gcgggaaggc acagggcgac
    20941 agcttattgc tggtgctttg caatatattc atgacctcga ctatctttcg gtgagttttg
    21001 gttacaccga ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg
    21061 gtaatcatcg tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg
    21121 atgcgggtaa acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc
    21181 tcgcgcagtg gaatggcgaa atgcttcctg ttgatccact aaacgatgcc gtcctttctg
    21241 acgacgactg gcttgaactg gccggttttg ctttcactca tcgtccgcta ttaacgtcgt
    21301 taggttgctt attgcgtctg ctacaaacca gcgaactggc attaccggcg ctgcgtgggc
    21361 gtttacagaa aaacgccagt gacgcgcagt tatgtaccac acttaaactt tcaggccgta
    21421 agctgttact ggttcgccag cgggaagagg ccgcgcaggc gctattcgca cttgatgatg
    21481 ttcgcactga gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc
    21541 aattttctcc catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag
    21601 ttcgccatga aacatgacca ttttgttgtt caaagcccgg ataaaccagc acaacagttg
    21661 ttactgcttt tccacggtgt cggggataac ccggtggcaa tgggggaaat aggtagctgg
    21721 tttgcgccgc tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt
    21781 aatccggcgg ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg
    21841 cgtgtgaatg ccatcatgcc gacgtttatt gagacggtgc gctactggca gaaacaaagc
    21901 ggagtagggg ctaacgccac ggcgctaatc ggtttctcac aaggcgcgat tatggcgctg
    21961 gagagcatta aagccgaacc gggccttgct tcgcgcgtca ttgccttcaa cggacgctac
    22021 gcgagcctgc cggaaaccgc gtctacagcc acaacaattc acttgattca tggcggtgaa
    22081 gatccggtta tcgatctggc gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt
    22141 ggtgacgtga cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg
    22201 caatttgccc tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta
    22261 agcggcggta agcctggcga tgatgatgtg attgaaatga tgtaataata gccgggtagg
    22321 tctgataaga ctgtatcagg catcatggcc ggatgcggcg ttaacgcctt atccggccac
    22381 ttagcttact tcttcggttg atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg
    22441 ttgaaatcac gatgcggggg aagctccggc ttgttggcga gaaatttctt atggtcgacg
    22501 cgcttgagat ctttaatcac gttcagcagg acgcctacca aaaagacaat caccagaatc
    22561 caccaatatt tagccagcca gtccatgctt atttcctctt gcagaaccac tcatcaggcg
    22621 acgagctgtt ccatgatacg ttgatacata cgggcaagta gctgcaggtc ggcagcgttc
    22681 acacattcat tgattttatg aatcgtggca ttgaccggcc cgagttccac cacctgcgcc
    22741 cccatgcggg caataaagcg cccgtcggac gttccgcctg tggtcagtag ctgcggttta
    22801 atttcattat agtgctcaac cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc
    22861 aaaaatggct gcccggaaag ccaccaatcc accgtatagc gcagttgatg tttttcaagc
    22921 agggcaagca cctgcgcttt gatcatctca tccgtcagtt cggtgctaaa gcggaagtta
    22981 aactgcacaa acagttcacc tggaataacg ttgttactgc ccgttcccgc ctgaatattg
    23041 gcaatctgca tactggtcgc cgggaagaag tcattaccct gatcccactc aatagccact
    23101 aattcattaa tgaaaggtgc tgcgcgatgt accggattgt cagccagatg tgggtaggca
    23161 acatgcccct gaacgccatg aatggtaagg ttgcaggtta acgatccgcg acgaccattt
    23221 ttcaccacat cacctaccac ttcgatactc gacggttcgc caaccagaca gtaatcgaga
    23281 cgctcattac gcgccattaa cgcttcgacg acttttaccg taccgttgtg ggcactagct
    23341 tcttcatcag aggtgatcag aaatgccagt cgccctgtat ggttgggatg ttgtgcgaca
    23401 aagcgttctg ccgctaccac catcgccgcc agcgagcctt tcatatctgc cgcacctcgc
    23461 ccaaataaca tgccgtcacg aatggttggt tcaaatggtg gattgatcca acggtcggcg
    23521 tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcta acgtttcacc ctgcccgcgc
    23581 catgcccaaa aattctgcgt atcggcaaag tccatgcgtt caacggtaaa accgatcgcc
    23641 tgcaaacgtt caatcaacaa agcctggcat cctgcgtcat caggactcag ggaagggcgg
    23701 cgaataagct gttgtgtcag ctcaataacc gggcacgaca tagactacac ctcatggaaa
    23761 aattgctgat aactggattc actgaaaccc agcagcatag gcttaccagg cgcgcagagc
    23821 aatggacgtt tgataattgc aggcatttca gtcattaatg tcgccgcaga ggccgcatcg
    23881 gtgattttat tgcgggtggt ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt
    23941 aacgcttccc agcctaattc gttgataaaa ccgttcaata attcgctgtc caatccatcg
    24001 acgcggtaat catgaaaacg atagtcgatg ttattggctt ccagccaacg gcgagccttt
    24061 ttaatggtgt cacaattttt gatgccgtaa
//
LOCUS       DACADA010000067        23564 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.068, whole genome shotgun sequence.
ACCESSION   DACADA010000067 DACADA010000000
VERSION     DACADA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.068"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            81..554
                     /locus_tag="ISB17_002529"
     CDS             81..554
                     /locus_tag="ISB17_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1980453.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDYDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            570..1046
                     /locus_tag="ISB17_002530"
     CDS             570..1046
                     /locus_tag="ISB17_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1980454.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1109..1330
                     /locus_tag="ISB17_002531"
     CDS             1109..1330
                     /locus_tag="ISB17_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1980455.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1349..1993
                     /locus_tag="ISB17_002532"
     CDS             1349..1993
                     /locus_tag="ISB17_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP1980456.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     RAVCGIFGIDLRQRVFRLSNGRFMEEACIWFSGFCSQGDGACFEGRWHWQPATARRLR
                     EYATQDHELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQAMT
                     ADAEAAVSEALRDLAFWLYRQLENEYDRLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            2009..2377
                     /locus_tag="ISB17_002533"
     CDS             2009..2377
                     /locus_tag="ISB17_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1980457.1"
                     /translation="MSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            2466..2840
                     /gene="cbtA"
                     /locus_tag="ISB17_002534"
     CDS             2466..2840
                     /gene="cbtA"
                     /locus_tag="ISB17_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HAP1980458.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTSDNYRTVNNITLGKYPEAK"
     gene            2837..3031
                     /locus_tag="ISB17_002535"
     CDS             2837..3031
                     /locus_tag="ISB17_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980459.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            3044..3157
                     /locus_tag="ISB17_002536"
     CDS             3044..3157
                     /locus_tag="ISB17_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1980460.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(3446..3592)
                     /locus_tag="ISB17_002537"
                     /pseudo
     CDS             complement(3446..3592)
                     /locus_tag="ISB17_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3646..3828
                     /locus_tag="ISB17_002538"
     CDS             3646..3828
                     /locus_tag="ISB17_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAP1980461.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVTSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(3929..4258)
                     /locus_tag="ISB17_002539"
     CDS             complement(3929..4258)
                     /locus_tag="ISB17_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAP1980462.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4430..5488)
                     /locus_tag="ISB17_002540"
     CDS             complement(4430..5488)
                     /locus_tag="ISB17_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP1980463.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVLETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(5686..6159)
                     /gene="sbmC"
                     /locus_tag="ISB17_002541"
     CDS             complement(5686..6159)
                     /gene="sbmC"
                     /locus_tag="ISB17_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HAP1980464.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQMVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6278..7444)
                     /gene="dacD"
                     /locus_tag="ISB17_002542"
     CDS             complement(6278..7444)
                     /gene="dacD"
                     /locus_tag="ISB17_002542"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HAP1980465.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7653..9080
                     /gene="sbcB"
                     /locus_tag="ISB17_002543"
     CDS             7653..9080
                     /gene="sbcB"
                     /locus_tag="ISB17_002543"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAP1980466.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9191..9649)
                     /gene="tnpA"
                     /locus_tag="ISB17_002544"
     CDS             complement(9191..9649)
                     /gene="tnpA"
                     /locus_tag="ISB17_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP1980467.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKCRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(9866..11224)
                     /gene="plaP"
                     /locus_tag="ISB17_002545"
     CDS             complement(9866..11224)
                     /gene="plaP"
                     /locus_tag="ISB17_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAP1980468.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11214..11276)
                     /gene="yoeI"
                     /locus_tag="ISB17_002546"
     CDS             complement(11214..11276)
                     /gene="yoeI"
                     /locus_tag="ISB17_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAP1980469.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11491..12420)
                     /locus_tag="ISB17_002547"
     CDS             complement(11491..12420)
                     /locus_tag="ISB17_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1980470.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12466..13290)
                     /locus_tag="ISB17_002548"
     CDS             complement(12466..13290)
                     /locus_tag="ISB17_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1980471.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(13373..13627)
                     /gene="yoeB"
                     /locus_tag="ISB17_002549"
     CDS             complement(13373..13627)
                     /gene="yoeB"
                     /locus_tag="ISB17_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAP1980472.1"
                     /translation="MKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAACRYHY"
     gene            complement(13624..13875)
                     /gene="yefM"
                     /locus_tag="ISB17_002550"
     CDS             complement(13624..13875)
                     /gene="yefM"
                     /locus_tag="ISB17_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAP1980473.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKDIIE"
     gene            14158..14208
                     /gene="hisL"
                     /locus_tag="ISB17_002551"
     CDS             14158..14208
                     /gene="hisL"
                     /locus_tag="ISB17_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAP1980474.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            14354..15253
                     /gene="hisG"
                     /locus_tag="ISB17_002552"
     CDS             14354..15253
                     /gene="hisG"
                     /locus_tag="ISB17_002552"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAP1980475.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            15259..16563
                     /gene="hisD"
                     /locus_tag="ISB17_002553"
     CDS             15259..16563
                     /gene="hisD"
                     /locus_tag="ISB17_002553"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAP1980476.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            16560..17630
                     /gene="hisC"
                     /locus_tag="ISB17_002554"
     CDS             16560..17630
                     /gene="hisC"
                     /locus_tag="ISB17_002554"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAP1980477.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            17630..18697
                     /gene="hisB"
                     /locus_tag="ISB17_002555"
     CDS             17630..18697
                     /gene="hisB"
                     /locus_tag="ISB17_002555"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAP1980478.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGAQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            18697..19287
                     /gene="hisH"
                     /locus_tag="ISB17_002556"
     CDS             18697..19287
                     /gene="hisH"
                     /locus_tag="ISB17_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAP1980479.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIEED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGTAGAKLLKNFLEM"
     gene            19287..20024
                     /gene="hisA"
                     /locus_tag="ISB17_002557"
     CDS             19287..20024
                     /gene="hisA"
                     /locus_tag="ISB17_002557"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAP1980480.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVTGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVFLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            20006..20782
                     /gene="hisF"
                     /locus_tag="ISB17_002558"
     CDS             20006..20782
                     /gene="hisF"
                     /locus_tag="ISB17_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAP1980481.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            20776..21387
                     /locus_tag="ISB17_002559"
     CDS             20776..21387
                     /locus_tag="ISB17_002559"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAP1980482.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNILNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLATVIENLRKRHQ"
     gene            complement(21573..22550)
                     /gene="wzzB"
                     /locus_tag="ISB17_002560"
     CDS             complement(21573..22550)
                     /gene="wzzB"
                     /locus_tag="ISB17_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAP1980483.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQATPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGVGIVLGRNALRNYNAK"
     gene            complement(22696..>23564)
                     /locus_tag="ISB17_002561"
     CDS             complement(22696..>23564)
                     /locus_tag="ISB17_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP1980484.1"
                     /translation="IKDVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLR
                     EGKALYDNLHPSRIVIGERSERAERFAALLQEGTIKQNIPTLFTDSTEAEAIKLFANT
                     YLAMRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQ
                     LLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSI
                     QGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVA
                     DKVYTRDLFGSD"
BASE COUNT         5938 a         6164 c         5859 g         5603 t
ORIGIN      
        1 cacggtcagt gatgaacgtg atgttgccag ctcccgacct ttactccctc tcatttattc
       61 acattacaag gatttcatac atgaaaacat tgtctcagaa caccacctct tcggcctgtg
      121 caccggagac tggcctgcaa caactggttg ccaccatagt ccctgatgaa cagcgcatca
      181 gcttctggcc gcagcatttt ggcctcattc cacagtgggt gaccctggag ccccgtgtct
      241 tcggctggat ggaccgtctg tgcgaaaact actgcggtgg tatctggaat ctgtacaccc
      301 tgaacaacgg cggggcattt atggcccctg aaccggatga ctatgatgac gaaacatggg
      361 tactgttcaa tgccatgaac ggtaaccgcg ctgaaatgag cccggaagcc gccggcattg
      421 ccgcctgtct gatgacgtac agtcatcacg cctgccgtac ggagtgttat gccatgacgg
      481 ttcattatta ccgtctgcgg gattacgccc tgcagcatcc ggaatgcagc gccattatgc
      541 gcattattga ctgaaagggg gccggaacaa tgcaacagat ttcctttctg cccggagaaa
      601 tgacaaccag agagcgcagc ctcattcagc gggccctgaa aaccctggac cgccatcttc
      661 atgaacccgg cgtggccttc acctccaccc gtgcggcacg ggaatggctg attctgaaca
      721 tggcgggact ggagcgtgaa gaattccggg tgctgtatct gaataaccag aatcagctga
      781 ttgccggtga aaccctcttc accggcacca tcaaccgcac ggaagtccat ccccgggaag
      841 tgattaaacg cgccctgtat cacaatgccg cagccgtggt gctggcgcac aatcacccgt
      901 ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg gtacaggcac
      961 tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcagc caggttttct
     1021 cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg ttttcacttt
     1081 tatcttctgt cttcagaggt atcccactat gaaaatcatc acccgtggcg aagccatgcg
     1141 tattcaccgt cagcatcctg catcccgtct ttttccgttc tgtaccggca aataccgctg
     1201 gcacggcagt gctgaagcgt ataccggtcg tgaagtgcag gatattcccg gtgtgctggc
     1261 cgtgtttgct gaacgccgta aggacagttt tggcccgtat gtccggctga tgagcgtcac
     1321 tctgaactga gtcagtaagg gcattctgat gagcagaatt atcaccacca ccgtgtatac
     1381 actgcatgag ctatcctcca ccgcacagga aaaagcccgc gactggtacc gtcagcatca
     1441 tgctgacagc aactggtatg aaaacgtgta tgaggacttc agggcggtct gtggcatttt
     1501 cggaattgac ctccgccagc gcgttttccg gctcagcaac ggcaggttta tggaagaggc
     1561 gtgcatctgg ttcagcggat tctgcagcca gggggacgga gcctgttttg aaggacgctg
     1621 gcactggcag ccagcaacag ccagaaggct ccgggagtat gccacacagg accatgaact
     1681 ccatcgtatc gcggatgccc tgcaggcggt gcagaaacgt aacttctggc aactgcaggc
     1741 tgaaatccac caccggggac gttactgcca tccgtacagc atggatatca ctgttacccg
     1801 gaacagtccg actggtcagg ctatgacagc cgatgcggaa gccgctgtca gcgaagccct
     1861 gcgcgacctg gccttctggt tgtaccgtca gctggaaaac gagtacgaca ggctgacctc
     1921 tgatgccgct gtggatgagg cccttctcat caacgaatac accttcaccg aagccggtct
     1981 gcgcgccggc tgacctgagg actttatcgt gtcagacaaa ctccatgaga caaattatcc
     2041 cgacgaccat aacgaccgcc tctggtgggg actgccctgc accgtgacgc cctgtttcgg
     2101 ggcacgtctg gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg ccggtatcag
     2161 aggcctgttc agcgatgcag atgcgtacca cctggaccag gcctttccgc tgctgatgaa
     2221 acaactggaa ctcatgctca ccagcggtga actgaatccc cgccatcagc ataccgtcac
     2281 gctgtatgca aaagggctga cctgcaaagc cgataccctc agcagttgtg gttacgttta
     2341 tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag ccttctgcta
     2401 cggcctggtg ttttcaccac gccacttttc catttttata tctgcatatc aggaaaatct
     2461 tcagtatgaa aacattacct gtattacccg ggcaggcggc cagttctcgc ccgtctcctg
     2521 ttgaaatctg gcagatactg ctgtcccgac tgctggacca gcactatggc ctcacactga
     2581 atgacacacc gtttgccgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     2641 tgtgtgatgc ggtgaacttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2701 gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc cgggctcgca
     2761 gggcgaccgg cctgatgacc agcgacaatt acagaacggt aaataacatt accctgggta
     2821 agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac aggcgctgga
     2881 tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga ttaataaggc
     2941 cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg aaacaccggc
     3001 aacttatgcc agccctcacc agttgctgta agacaatgca gtgatacagg gtatagtgcc
     3061 attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag atgtccttac
     3121 cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca ggcttcattt
     3181 cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa ggaaaaccga
     3241 taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg ataaaccttc
     3301 cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa aaatgaattc
     3361 caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga ttaaagtaag
     3421 cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc catggcagga
     3481 gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg cgcagatacg
     3541 cttccggatc gataccgttc agacggcagg tgcctgcttt tccggcaaca agcaggaatc
     3601 tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa tcacccggtc
     3661 attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt ggtgacaagt
     3721 tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcagttaa taacaatgga
     3781 gttacagaac tttttgcctt gctgcataca gaagaaggct gttgttaatt tcgattggtt
     3841 ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc
     3901 gggcaaccct cattgaataa aacgggaatt acttcgcttc gccgtttttc atttcgccca
     3961 tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga
     4021 tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg
     4081 cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct
     4141 tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac
     4201 gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt
     4261 ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca
     4321 acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt
     4381 tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca
     4441 aggcccggca aatcagattc gatagcaact caagctgatg tgccgtttcc atgttcagcc
     4501 acacataacc atagattggt gtttccaaga ctttcaggtc atggtggtta tcgagcaact
     4561 gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat
     4621 taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat
     4681 cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat
     4741 tgatttgcaa ctccagcata caaaccagat tgcgattaat ggtctggata ccttcatata
     4801 tcgatttggg aatacgggtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg
     4861 catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg
     4921 agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc
     4981 agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga
     5041 ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa
     5101 caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc agcgccagcc
     5161 aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac gaaattaact
     5221 ccagttgcag agcgataaga cctaaaatcg aacccaacac cgtaccgcca atacgttcga
     5281 aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc accatggtga
     5341 ccagcggcca ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga
     5401 atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa
     5461 acgggcttaa tgacttatcg gcacgcacgc agaaactccg taatgaagat gaaaagcgct
     5521 cataacacat ctgtcggcca gaaggcgcgc ctctgagtta tccgacggac ataccgggaa
     5581 cagtgtaatt tcccggcagg agcgaaaaat ttgaaaggcg catcttactc ttttcgcttt
     5641 caaaaaaaga gtggtcttcg cgttaacaca ccgccctgag atgaattagt gatgttttgg
     5701 ctgcaccgca acatacattt cgatatccca gtacccatct tccgcgccat tgttcaagta
     5761 aacctcgaag cagggctttg gtaacatttc ataagcactg tcctgcaaga ggctattaaa
     5821 gaactgatac cagggtttag caaaatcatc accgactaca cgagccaccg ccaccgcata
     5881 ctgaccacct gaaatttctg tcagaatgac gccctcactg ttttcgggga gcgtaaagtt
     5941 attcggtacc gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt
     6001 gtcgtaatag acagcaaccc actccttcgg cacaatattt ttgctatcta tccacatcat
     6061 caactgctca aaacctttct ttaccatctg ctcccacggg ccaacaagat ggaaacctgc
     6121 aacggtacgt ttatcttcct gcttaatctc gtagttcatg acgcctccac taacactgtt
     6181 tttatataca gtatagttgc aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc
     6241 agactcgcac tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat
     6301 cactcaggcg ggaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt
     6361 gcgccacctg tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa
     6421 ttggcgcggt gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg
     6481 ctttcggtag caccatccag aactcttgtt ccgttcccag ggcgatattt tctttatcac
     6541 cataccagat gcgttccgta ccgacctttt tcccacggtg caaaatttgc accgtagtaa
     6601 agttttgttg cccccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcgctgt
     6661 cagcccccat taccactgca atgaggcgac gctgcccgtc tacagccgaa gcaataagat
     6721 taaacccggc accagaagta tggcccgttt tcaggccgtc aacattcatg gttttatccc
     6781 acaataaccc gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt
     6841 acatatgata aaactcgggc tcgccgtgga tgatagcgcg agaaagcacg gctaaatcat
     6901 aagccgagct atgctggcca ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct
     6961 tgagatgcag cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg
     7021 caatatagtc agccagagca acacaagcgt catttccgga atccacaatt aaaccacggc
     7081 ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa
     7141 acaccggatt atctttcgcc cacgcatcac gccccacggt gacaatatcg tctggcgtaa
     7201 tgcgatgact atcgatagcg cgatccacga catagcccgt catcagcttt gtcaggctgg
     7261 cgggattgcg ctgttgatgc tcattacccg cggtgagaat ctgaccggtg gtgtaatcca
     7321 tcaataccca ggacccggca tgaatctctg gaggctgagg tgaaaaagga atgttttccg
     7381 ccgcaaaacc agacgataaa ttaaaaacga acaaagaagc agcaataata agacggcgtt
     7441 tcaacagcaa accctcaggt gtttcaaata gctgttcttt ttacggaaat acttatgaac
     7501 tggctggaat aaagtgcaag aaaatgtgac tactatctca tttttatctg acatgatctg
     7561 ttgccactcg ctgccaaatt gtcgcgctaa agctgattag cacggtgata tttgatactc
     7621 tggcagacag cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta
     7681 cctttttgtt tcacgattac gaaacctttg gaacgcaccc cgcgttagat cgccctgcac
     7741 agttcgcagc cattcgcact gataacgaat tcaatgtcat cggcgaaccc gaagtctttt
     7801 actgcaagcc cgcggatgac tatttacccc agcctggagc agtattaatt accggtatta
     7861 ccccgcagga ggcacgggcg aaaggagaaa acgaagccgc gtttgccgcc cgtattcact
     7921 cgctttttac cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgacg
     7981 aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc
     8041 atgataactc gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg
     8101 aaggaataaa ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt gagcatttaa
     8161 ccaaagcgaa tggtattgaa catagcaacg cccacgatgc gatggctgat gtgtacgcca
     8221 ctattgcgat ggcgaaactg gtaaaaacgc gtcagccacg cctgtttgat tatctcttta
     8281 cccatcgtaa taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctag
     8341 ttcacgtttc cggaatgttt ggggcatggc gtggcaatac cagctgggtg gcaccgctgg
     8401 cgtggcatcc tgaaaatcgc aatgccgtaa ttttggtgga tttggcagga gacatttcgc
     8461 cattactgga gctggatagc gacacattgc gcgagcgttt atataccgca aaagccgatc
     8521 ttggcgataa cgccgccgtt ccggttaagc tggtgcatat caataaatgt ccggtactgg
     8581 cccaggcgaa tacgctacgc ccggaagatg ccgaccgact gggaattaat cgtcagcatt
     8641 gcctcgataa cctaaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga
     8701 tattcgcgga agccgaaccg tttacacctt cagataacgt ggatgcacag ctttataatg
     8761 gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta
     8821 atttaccggc actggatatc acttttgttg ataaacgaat tgaaaaactg ttgttcaatt
     8881 atcgagcgcg caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc
     8941 accgccgcca ggtcttcacg ccagagtttt tgcagggcta tgctgatgaa ttgcagatgc
     9001 tggcacaaca gtatgccgat gacaaagaga aagtggcgct gttaaaagcg ctttggcagt
     9061 acgcggaaga gatcgtctga aaataaatat cctccggcat agccggaggt ttttcaaatg
     9121 cgcctttaag gctctcttac cagccgcgcc ctaacaggcg cacacgatct gacatttgca
     9181 tccaacttcg ttacttacgg cccgtaaacg ggctgcccgg atagggaatc gataactgct
     9241 ctcccatttt atcctcttca agctggtgct ttatgtaatc ctgtatcttc gccgtgttct
     9301 tacccaccgt atcgacgtaa taccctctgc accagaactc cctgttcctg tatttgaatt
     9361 tcaaatcacc aaactgctcg taaggcatca gactgctttt ccctttcaga tatcccataa
     9421 agcctgatac gctcattttg ggcgggatct ccacaagcat atggatatga tctgcacagc
     9481 attcagcttc cagaatccgt acacttttcc actcacacag ctttctcaaa atacagccta
     9541 ttgctctaca cttctctctg tagaacacct gtcttcggta ttttggcgca aatactatgt
     9601 gatatttaca gttccatcgg gtgtgcgcta agctcttttc gttccccatt gggaccccct
     9661 tttgatttct tgtttgacac ttgcagttgc cagaccgcaa ggtgttttaa caaatcaaaa
     9721 ggggttttaa taactggctc aaagctgaaa gctttccgga acccccagcc tagctggggg
     9781 ttttctgtgc acaacaaaaa agccggagcg acaaactccg gctttttgag atttaccgga
     9841 tgattatgtc atccggtttg taagattacg ctacgtcttc gtactgcgga accgggttac
     9901 ggaaactctt ggttacgcaa gccaggtaaa tcagaccgat agcagcccag atcagaccca
     9961 gaaccattga gctttcttcc aggttaaccc acagcgcacc aacggtcagc gcaccacaca
    10021 tcggcaggaa cagatactgg aagtgatctt tcagcgtttt gttacgcttc tcacggatcc
    10081 agaactgcga gatgaccgac aggttaacga aggtgaacgc caccaacgca ccaaagttaa
    10141 tcagcgccgt agccattacc aggtcgaagt tgattgccag cagggcaatc gcgccaacca
    10201 ggatgatatt catcgccgga gtacgccatt tcgggtgtac ataaccgaag aagcttttcg
    10261 ggaacacgcc gtcacgaccc attacgtaca tcagacgcgc aacgcctgcg tgcgccgcca
    10321 taccggacgc cagtacggta atggtggaga agatcagcgc gccaacctgg aacgctttac
    10381 ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg atctttaaaa cgagagatat
    10441 ccgggaagta cagctgcagg aagtaagttg caaagatgaa gatcatgccg ccgatcagcg
    10501 cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc tttggtttct tccgacaggt
    10561 tgctgatgcc gtcaaaaccg gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg
    10621 ggataacatg cgcatcgcca gaccagaacg gacgagtgct cgccagcgta ccagcgcctt
    10681 caccttcaaa tacgccataa acaaccatgc ccagaatcac cgcgatcagc actatctgca
    10741 acacgacgat tacagtgttg aagttcgcta cggatttcag actacgcagg ttaaaggcgg
    10801 tcataaaggc caccagagcc accacaaaca tccacgatgg aatggaaggc accagagctt
    10861 caaaatagat tttcgccagc agaatgttga tcatcggcgc gaacagatag tcgagcagag
    10921 aagaccaacc caccataaag ccgacagtcg ggctaatgga tttctgggcg taagtgtatg
    10981 cagagccagc agaaggatag cggcgaacca gcttcccgta gctcagagcc gtaaacagga
    11041 tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc atccgtgagg cctgaaacga
    11101 taccaaatgt atcaaacagc gtcatcggct gcatataggc tagacccatc attacaaccg
    11161 gaactaacgt aagcgtttta cgcaattcca cgcgagaggt gtttggagta acgttatgcg
    11221 acatggtcat tctcctttac ggtgataacc gtcgcgtaag caaaaaattg ccccattttt
    11281 ttggattcct cagcgacaac aactgtcgat ttttagtaaa tatctatccg gtacgaagcc
    11341 cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata accgacgcta attaaacgtc
    11401 ggtttttagt cattttttga cagcggcgca ttgtgcctaa atggggggga aatgacaaga
    11461 gaatgagagg cttgccataa taatttttct ttaaatggct gattttccgt catcagattt
    11521 tgcataaaca ccggcgatcg cactatttgc taaaatttca tctcgccacc agccgctggc
    11581 aagccctgcg gtctgttcat tccagccaat ccagacgggt tcaaagagag tttgggcgac
    11641 cactttcttc tcgattaacg cgccactatc gagaaaacgt tgcgccagat accgtggtaa
    11701 atagccgcag ccaaggccgc taatttgcag ctccagcttg gttttaaaat cgaaaacggt
    11761 aatggcttct tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc
    11821 ccccaccaca atcgcccgat atcgtttaat gatacgacgg tttaacggtt cttcttcctg
    11881 ggctaacgga tgatgaggcg cgaccgcaaa gacttgttca agatcgccca accgcgagaa
    11941 accaaactca ctggaagatg gtggctcatg catcgcgcca acgataatat ccgctcgccc
    12001 ctgagtcagg gcatcccagg aaccaccgag tacgccattg ataaacttca ggcgcgtcac
    12061 gctatgatgt tgataaaaag cttcaatgag aggcgcaagc agagaaaaag gaaacgtgtc
    12121 gtccacgcca atcaccagct cgttttccca gccttcatgc agtttgatcg cctgcttttc
    12181 cagttctcgc acggtatgca gaacttcccg ccctttctct aataacattt ttccggtacg
    12241 ggtgaattta gcgcggtgtc cgctacgatc aagcaattga atattaaggt cgctttccag
    12301 cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag agtttggctg acgccgccgc
    12361 aaaactgcct tctttttcta aggcatcgag gatcatcaac acatccagca gtggtttcat
    12421 acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat
    12481 taccagcgga tcgggatact ggtattcaaa tcccagttca ttacaaatcc gactgccatc
    12541 aataatctta cctttgccgc tgtccagact atttctgaac tgcggaggtt ccagccccag
    12601 taaacgggcc atctgcggat agaaaacatt acgcgcaggg tgagctggcg cacatatatt
    12661 atagatgtgt ccgcctttag gtgcctgtaa caacagagtg atagcgccaa taacatcttc
    12721 taaatggact aaattaacac catgttcacc atcaggggcg gtttttccgg caaagaagcg
    12781 tccgggatga cgtcccggtc cgaccaggcc cgcaagacgc agaatatcga ccgaagtacc
    12841 gggtaaattg tgcagccagt cttcgagttc ttctaatact cgtccactgt tggttaccgg
    12901 attacgcggg gtggtttctt tcaccgtgcc ttgtgcgtcg ccatagacag acgtcgagct
    12961 ggtaaaaata atacggggaa tacgatgagc cagcgcgcta tccactaact cttgtaccgc
    13021 ttgtaaatag aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc
    13081 atcggcatcc ataagggcat ccagatcgtc agaatcgcaa actaactcag gctccatgcg
    13141 aagcagatag ctatcaatgc cactcattcg ggccgcttcg acaccatctt gtgtggtttt
    13201 actcccggtg acttgccagc ctcgcgctga aagtgacatc gccagcggca tacctaacca
    13261 ccctaacccg acaattgcga cctttttcat ccgttatctc ctgatacacc ttagatctat
    13321 aaggctacgc tagcgtatca aaactgacaa ttcattctat gaatgaatct cttcaataat
    13381 gataacgaca tgctgcaatg agcagtgaat catcagtaac cgcgtatacc agaaggtgct
    13441 cctctgtaat gcgtcgggac cagaaacctg acaagttatg tttcaggggt tctggtttcc
    13501 ccttaccttc aaatggtgtt ctgcgggtat ctttgataag ttcattgatc tttttaacaa
    13561 ttcgcttatc tgcttcctgc cagtacagat aatcatccca tgattcctca gaccagatta
    13621 gtttcactca ataatgtcct tttccgttcc tttgtctgat ttcaggctat cgattgagtc
    13681 catcaatctc cgggcgttag cgggggaacg cagtagataa gccgtctctt ccagcgagtt
    13741 gtattcttcg agtgacatca gaacacaagc ctctccattc tgacgagtaa tgaggatcgg
    13801 ggcatgatct tcaacggctt tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta
    13861 gctaattgta cgcatgtaaa tctcctcttt tgtacagttc attgtacaat gatgggtgtt
    13921 aattaactat ttattaatta gtttgtagat caagacattg ccagcgagac gaaaaatcag
    13981 actttgctct ttttggtgct gtcaagttag aggacagtcc tcttagcccc cttctttccc
    14041 cgctcattca ttaaacaaat ctatcgccat aaaatatata aaaaagccct tgctttctaa
    14101 cgtgaaagtg gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg
    14161 acacgcgttc aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga
    14221 tgtgtgctgg aagacattca gatcttccag tggcgcatga acgcatgaga aagcccccgg
    14281 aagatcacct tccgggggct tttttattgc acgattgata acggttcaga caggtttaaa
    14341 gaggaataag aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg
    14401 tttaagtgat gactcacgcg aattactggc gcgctgtggc attaaaatca atcttcacac
    14461 ccagcgtctg atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga
    14521 cgatattccc ggtctggtaa tggacggcgt ggtagacctt gggattattg gcgaaaacgt
    14581 gctggaagaa gagctgctta accgccgcgc ccagggtgaa gatccgcgct actttaccct
    14641 gcgtcgtctg gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg
    14701 ggacggtccg ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa
    14761 gcgttatctc gaccagaaag gtatctcttt taaatcctgc ttactgaacg gttctgtcga
    14821 agttgcgccg cgtgccgggc tggcggatgc gatttgcgat ctggtttcca ccggtgccac
    14881 gctggaagct aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgcctgat
    14941 ccagcgcgat ggcgaaatgg aagaatccaa acagcaactg atcgacaaac tgctgacccg
    15001 tattcagggt gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga
    15061 acgcctggat gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct
    15121 ggcgggtgac caacagcgcg tagcgatgca catggtgagt agcgaaaccc tgttctggga
    15181 aacgatggaa aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa
    15241 gatgatggag tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg
    15301 gagcaacaac gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc
    15361 accgttaacg atattctcga taacgtgaaa acgcgtggcg atgaggccct gcgtgaatac
    15421 agcgcgaagt ttgataaaac cacggttacc gcgctgaagg tgtctgctga tgagatcgcc
    15481 gccgccagcg aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat
    15541 attgaaacct tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc
    15601 gtgcgttgcc agcaggtcac gcgcccggtg gcttcagtgg gtttgtatat tcctggcggc
    15661 tccgccccgc tcttctcaac ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt
    15721 aaaaaagtgg tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcaa
    15781 ctgtgcggtg tgcaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg
    15841 tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc
    15901 accgaagcaa aacgccaggt aagccagcgt ctggacggtg cggcgatcga tatgcccgca
    15961 ggcccgtcgg aagtgctggt gattgctgac agcggcgcaa cgccggattt cgtggcttct
    16021 gatttgctct cccaggctga acacggcccg gactcacagg tgattttact gacacctgat
    16081 gctgatatgg cgcgtcgcgt tgccgaggcc gtcgaacgcc agttggcaga attgccgaga
    16141 gctgaaaccg cccgtcaggc actgagcgcc agccgcctga tcgtaaccaa cgatttagcg
    16201 cagtgcgtag agatctccaa tcagtacggt ccggagcacc tgatcattca gacccgcaac
    16261 gcccgcgatc tcgtcgatag cattaccagc gccggttcgg tatttctcgg tgactggtca
    16321 ccggaatcgg caggtgatta cgcctccggc accaaccacg tgctgccgac ttacggttac
    16381 accgccacct gttccagcct cgggctggca gatttccaga aacgcatgac cgtacaggaa
    16441 ctgtcgaaag aaggtttctc cgcgctggct tcgaccattg aaacactggc cgccgccgag
    16501 cgcctgaccg cccacaaaaa tgccgttact ttgcgtgtta acgtccttaa ggagcaagca
    16561 tgagcaccgt gactattact gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc
    16621 agtcggcgcg tcgtctgggc ggtaacggcg acgtctggct gaacgccaac gaatatccca
    16681 cagccgtgga gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgccagccaa
    16741 aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca
    16801 gccgtggcgc ggacgaaggt attgaactgc tgatccgcgc tttttgcgaa ccgggtaaag
    16861 acgccatcct ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg
    16921 gcgtcgaatg ccgcacagtg ccgacgctgg aaaactggca actggactta cagggcattt
    16981 ccgacaagct ggacggcgta aaagtggtct atgtttgcag ccccaacaac cccaccgggc
    17041 aactgatcaa cccacaggat tttcgcaccc tgctggagtt aacccgcggt aaggcgattg
    17101 tggttgccga tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctgg
    17161 cggaatatcc gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcggggc
    17221 ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg
    17281 ccccctaccc gctctcgacg ccagttgccg acattgccgc acaggcgtta agcccgcagg
    17341 gaatcgtcgc catgcgcgaa cgggtggcgc aaattattgc agaacgcgaa tacctgattg
    17401 ccgcactgaa agagatcccc tgcgtggagc aggttttcga ctctgaaacc aactacattc
    17461 tggcgcgctt taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct
    17521 tacgtgatca gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc
    17581 gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa
    17641 gtatcttttt atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt
    17701 ggaccgtttt gacaaactcg cctttgaacc tggcgtgatc ccggagctgc tgaagctgca
    17761 aaaagcgggc tataagctgg tgatgatcac caatcaggat ggtctgggag cacaaagttt
    17821 cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg
    17881 cgtgcagttt gatgaagtgc tgatttgccc acacctgccc gccgatgagt gcgactgccg
    17941 taagccgaaa gtaaaactgg tggagcgtta tctggctgag caagcgatgg atcgcgctaa
    18001 cagttatgtg attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa
    18061 tggtttacgc tacgaccgcg aagccctgaa ctggccaatg attggcgagc aactcactaa
    18121 acgagaccgt tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt
    18181 gtggctggat cgcgagggtg gcagcaagat taacaccggc gttggcttct ttgatcacat
    18241 gctggatcag atcgccaccc acggcggttt ccgtatggaa atcaacgtta aaggcgacct
    18301 ctatatcgac gatcatcaca ccgtcgaaga taccggcctg gcgctggggg aagcattaaa
    18361 aattgctctt ggcgacaaac gcggtatttg ccggtttggt tttgtgctgc cgatggacga
    18421 atgccttgcc cgctgcgcac tggatatctc tggtcgcccg cacctggaat ataaagccga
    18481 gtttacctac cagcgtgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc
    18541 gctctcatac accatgggcg tgacgctaca cctgaaaacc aaaggtaaaa acgatcacca
    18601 ccgtgtagag agcctgttca aagcgtttgg ccgcaccctg cgccaggcca tccgcgtgga
    18661 aggcgacacc ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatact
    18721 ggctgcgcca acctgaactc ggtgaagtct gccattgcgc gtcacggtta tgaacccaaa
    18781 gtcagccgtg acccggacgt cgtgttgctg gccgataaac tgtttttacc cggcgttggc
    18841 accgcacaag cggcgatgga ccaggtgcgc gagcgcgagc tgtttgatct catcaaagcc
    18901 tgtacccaac cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagcgaa
    18961 gagagcaacg gcgtcgactt gctgggcatc atcgaagaag acgtgccgaa aatgaccgac
    19021 tttggtctgc cactgccaca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc
    19081 ctgtttcagg ggattgaaga cggcgcgtac ttttactttg ttcacagcta cgcaatgcca
    19141 gtcaatccgt ggaccatcgc ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa
    19201 aaagataact tctacggcgt gcagttccac ccggagcgtt ctggtaccgc tggcgctaag
    19261 ttgctgaaaa acttcctgga gatgtgatga ttattccggc attagattta atcgacggca
    19321 ccgtggtgcg tctccatcag ggcgattatg gcaaacagcg cgattacggt aacgacccgc
    19381 tgccgcgctt acaggattac gccgcacaag gtgccgaagt actgcacctg gtggatctga
    19441 ccggggcaaa agatccggct aaacgtcaaa tcccgctgat taaaaccctg gtcacgggcg
    19501 ttaacgtccc ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac
    19561 tggaagcagg cgttgcgcgt gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg
    19621 tgaagggctg gtttgaacgt tttggtgccg atgccttagt gctggcgctg gatgtccgta
    19681 tcgacgagca aggcaacaag caggtggcag tcagcggctg gcaagagaac tcgggcgttt
    19741 cactggaaca actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg
    19801 atatctcgcg cgacggcacg ctggcgggat ctaacgtctt tttatatgaa gaagtgtgtg
    19861 ccagatatcc gcaagtggcg tttcagtcct ccggcggcat tggcgatatt aacgatgtgg
    19921 cggccctgcg tggtactggt gtgcgcggcg taatagttgg tcgtgcatta ctggaaggta
    19981 aattcaccgt gaaggaggcc atcgcatgct ggcaaaacgc ataatcccgt gtctcgacgt
    20041 tcgtgatggt caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat
    20101 cgtgccactg gcaaaacgct acgccgaaga aggtgcagat gaactggtgt tctacgatat
    20161 caccgcttcc agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt
    20221 aatcgacatt ccgttttgtg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat
    20281 tctttccttt ggcgcagata aaatctccat caactctcct gcgctggcgg acccgacgtt
    20341 aattactcgc ctggccgatc gctttggcgt gcagtgtatt gtggtcggta ttgatacctg
    20401 gtacgacgcc gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac
    20461 ccgcgttact cagtgggaaa cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg
    20521 agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgagca
    20581 gctgaaaaaa gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcgggcac
    20641 catggaacac tttctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc
    20701 cgtattccac aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg
    20761 cgtggagatc aggatatgtt aacagaacaa caacgtcgcg aactggactg ggaaaaaacc
    20821 gacggtctga tgccggtgat tgtgcaacac gcggtatccg gcgaagtgct aatgctgggc
    20881 tatatgaacc cggaagcctt agacaaaacc ctcgaaagcg gcaaagtcac cttcttctcg
    20941 cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg gcaatattct caatgtggtg
    21001 agtattgccc cggactgcga caacgacacg ttactggtgc tggcaaatcc catcggcccg
    21061 acctgccaca aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctt
    21121 tatcaactgg aacaactgct cgccgaacgt aaatctgccg acccggaaac ctcctacacc
    21181 gccaaactgt atgccagcgg cactaaacgc attgcgcaga aagtgggcga agaaggcgtg
    21241 gaaaccgcac tggcagcaac ggtacatgac cgctttgagc tgaccaacga ggcatctgac
    21301 ttgatgtatc acctgctggt gttgttgcag gatcaggatc tggatttagc gacggtaatt
    21361 gagaacctgc gtaaacggca tcagtgagtt gcgtgctgac cggatgcgat gttgttgtcg
    21421 catccggagc cattgcccga tgcgcaagct tatcgtgcct acacattatc tcaacacacg
    21481 taggccggat aagatgcgcc agcatcgcat ccggcaaaaa aaacgggcat ggtgtcacca
    21541 ccctgcccgt ttctcttaaa tgcacaataa tattatttcg cgttgtaatt acgcagcgcg
    21601 ttacgcccca acacaatccc cacgccaacc atgccgccca gcagcaccgc cagaatcaag
    21661 gtaattgcct ttttcgggct atcgcgacga ataggtaacg tcggtttcat tacatagcgg
    21721 taagcatgaa tatcaagttt atcaacgtca agattgtcga tatccagcag gttttgacga
    21781 gtctgatagt agcttgatga gaacaccaac ggacgggtcg cctcgtgctt aatcatcgac
    21841 tccagcgctt cgctccccaa aaggaacata gtatcctggg ttacgtcctg cgtctgttga
    21901 atctgcggct ttgtcacctg cgcctgattc gcatactgca acgcttcctg aatctgacgg
    21961 atacgcagat ctttctgttc ctgcgcgacc acttcctggg ttcttaaaga gtcctgcaag
    22021 tttttccgtc ccagagcaat gttgtccttg agatcctttt ctagctcttg attcacttta
    22081 tcatcaactt gctgaatgta ttgggccaac ttcatttgtg cgccctctgc agtttgccca
    22141 acataagaaa cagtcaatgg taattgctgg ttcttaacag aaggttcgat ggtaagtttt
    22201 tctggctctt cctgattatc cagcgtttct gctaatgcag agaaggcaga actgaagcga
    22261 ccaattaacg tctcctgcaa atccgatact ttcggtgtag cctgaccata gataacattc
    22321 atggcattgt tatagccagc aatttgcccc acgtcgggct gagtgataat tgctgttgac
    22381 gtccatttct ccttcgctac tgccaaataa ccaatagcca gggcaatagc cacaatgacg
    22441 gaaatgatga ttgtcatctt gccacgccac aactgcacta gtaaatcaat caaatcaatc
    22501 tgttccgggt catggttttg cccagaaaca ttattatttt ctactctcat cattacccta
    22561 actgacggaa atttccaagt ccgcatagtt tagcgataat cctcgtgaat gctataggat
    22621 aagtgataaa aataatgaat tgttgaggaa gtaacaaaag acaatcaggg cgtaaatagc
    22681 cctgataaca agatgttaat cgctaccaaa gagatcgcgg gtgtatacct tatctgccac
    22741 atccttaagc tcttctgcca tacgattgga gataatgaca tcagcttgtt gtttgaaagt
    22801 ggcgagatct cgttccaggc gagagttgaa gaatgagtct tctttcatca ccggttcgta
    22861 aatgatcact tcaacgcctt tcgccttgat acgtttcata atcccctgaa tggaagacgc
    22921 gcggaagttg tctgaaccgc tcttcataat cagacgataa atacccacca cttgcggctt
    22981 gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg cggttagcat cgacaattgc
    23041 cgaaatcagg ttattcggca cagactgata attcgccaat aactgtttgg tatctttcgg
    23101 caaacaataa ccaccataac caaacgacgg attgttgtaa tggttgccga tacgcgggtc
    23161 gagacaaaca ccttcgataa tctgacgcgt attcagaccc aaactttctg catagctatc
    23221 cagttcgtta aagtacgcca cgcgcatcgc cagataggta ttagcgaaaa gtttaatcgc
    23281 ttctgcttca gtggagtcgg taaacagggt cgggatattt tgcttaatcg tgccttcctg
    23341 taacaacgca gcaaaacgtt ctgcgcgttc tgaacgctca ccgatgacaa tacgtgacgg
    23401 atgaaggtta tcgtaaaggg ctttaccctc acggagaaat tccggggaga atataatatt
    23461 ttcagtacga tattttttat gcatcgctgc ggtaaaacca acgggaaccg ttgatttgat
    23521 gaccataacc gcataagggt ttatctcaac tacgtcttta atta
//
LOCUS       DACADA010000068        22481 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.069, whole genome shotgun sequence.
ACCESSION   DACADA010000068 DACADA010000000
VERSION     DACADA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.069"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            45..929
                     /gene="xapR"
                     /locus_tag="ISB17_002562"
     CDS             45..929
                     /gene="xapR"
                     /locus_tag="ISB17_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HAP1980485.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1040..1115)
                     /locus_tag="ISB17_002563"
     tRNA            complement(1040..1115)
                     /locus_tag="ISB17_002563"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1080..1082),aa:Lys,seq:ttt)
     gene            complement(1120..1195)
                     /locus_tag="ISB17_002564"
     tRNA            complement(1120..1195)
                     /locus_tag="ISB17_002564"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1160..1162),aa:Val,seq:tac)
     gene            complement(1242..1317)
                     /locus_tag="ISB17_002565"
     tRNA            complement(1242..1317)
                     /locus_tag="ISB17_002565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1282..1284),aa:Val,seq:tac)
     gene            complement(1362..1437)
                     /locus_tag="ISB17_002566"
     tRNA            complement(1362..1437)
                     /locus_tag="ISB17_002566"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1402..1404),aa:Val,seq:tac)
     gene            1696..3111
                     /gene="gltX"
                     /locus_tag="ISB17_002567"
     CDS             1696..3111
                     /gene="gltX"
                     /locus_tag="ISB17_002567"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP1980486.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(3162..3554)
                     /locus_tag="ISB17_002568"
     CDS             complement(3162..3554)
                     /locus_tag="ISB17_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP1980487.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3556..3900)
                     /locus_tag="ISB17_002569"
     CDS             complement(3556..3900)
                     /locus_tag="ISB17_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP1980488.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            4136..4211
                     /locus_tag="ISB17_002570"
     tRNA            4136..4211
                     /locus_tag="ISB17_002570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4169..4171,aa:Ala,seq:ggc)
     gene            4251..4326
                     /locus_tag="ISB17_002571"
     tRNA            4251..4326
                     /locus_tag="ISB17_002571"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4284..4286,aa:Ala,seq:ggc)
     gene            4533..6722
                     /locus_tag="ISB17_002572"
     CDS             4533..6722
                     /locus_tag="ISB17_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAP1980489.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6772..7974)
                     /gene="nupC"
                     /locus_tag="ISB17_002573"
     CDS             complement(6772..7974)
                     /gene="nupC"
                     /locus_tag="ISB17_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAP1980490.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8310..9548
                     /gene="mntH"
                     /locus_tag="ISB17_002574"
     CDS             8310..9548
                     /gene="mntH"
                     /locus_tag="ISB17_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAP1980491.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(9689..10015)
                     /locus_tag="ISB17_002575"
     CDS             complement(9689..10015)
                     /locus_tag="ISB17_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP1980492.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(10130..11386)
                     /locus_tag="ISB17_002576"
     CDS             complement(10130..11386)
                     /locus_tag="ISB17_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAP1980493.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11590..12555
                     /gene="glk"
                     /locus_tag="ISB17_002577"
     CDS             11590..12555
                     /gene="glk"
                     /locus_tag="ISB17_002577"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAP1980494.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            12774..13100
                     /locus_tag="ISB17_002578"
     CDS             12774..13100
                     /locus_tag="ISB17_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAP1980495.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            13122..14369
                     /locus_tag="ISB17_002579"
     CDS             13122..14369
                     /locus_tag="ISB17_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP1980496.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            14384..15469
                     /gene="ypdF"
                     /locus_tag="ISB17_002580"
     CDS             14384..15469
                     /gene="ypdF"
                     /locus_tag="ISB17_002580"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP1980497.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            15469..16506
                     /gene="ypdE"
                     /locus_tag="ISB17_002581"
     CDS             15469..16506
                     /gene="ypdE"
                     /locus_tag="ISB17_002581"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP1980498.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            16531..19026
                     /gene="ptsP"
                     /locus_tag="ISB17_002582"
     CDS             16531..19026
                     /gene="ptsP"
                     /locus_tag="ISB17_002582"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1980499.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(19029..19886)
                     /locus_tag="ISB17_002583"
     CDS             complement(19029..19886)
                     /locus_tag="ISB17_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1980500.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(19899..20633)
                     /locus_tag="ISB17_002584"
     CDS             complement(19899..20633)
                     /locus_tag="ISB17_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1980501.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSCSKVKEFRQLMHL"
     gene            complement(20648..22345)
                     /locus_tag="ISB17_002585"
     CDS             complement(20648..22345)
                     /locus_tag="ISB17_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP1980502.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5360 a         5546 c         5999 g         5576 t
ORIGIN      
        1 atgataattt aatacgatgt atttattata tggagcactt aattatggaa cgcgtatata
       61 gaacagatct taagttgctc cgttattttc ttgccgtagc ggaagagttg cattttggtc
      121 gcgcagcagc gcgtttaaat atgtctcagc ctccgctcag tattcatatt aaagagctgg
      181 aaaatcaact cggcacgcag ctttttattc gccattcgcg cagcgtcgta ctgacacacg
      241 cgggcaaaat cttgatggaa gaatcgcgtc gattgctggt gaatgcaaat aatgtattgg
      301 ctcgggttga acaaataggt cggggagaag cagggcggat tgaactcggg gttgtgggaa
      361 cggcaatgtg gggacggatg cgcccggtta tgcggcgatt ccttagagaa aatcctaacg
      421 ttgaagttct gtttcgcgaa aagatgcccg cgatgcaaat ggccttgctg gaacgccgcg
      481 aacttgatgc cgggatctgg cgaatggcga cagaaccacc gactggtttt accagcttac
      541 ggttgcatga atcggcgttt ctggtggcga tgcctgaaga gcatcatctc tcatcatttt
      601 ccaccgtccc gctggaagcg ctacgtgacg agtatttcgt tacaatgccg cccgtgtaca
      661 ctgactggga ttttttgcag cgagtttgcc agcaggtggg attttcaccg gttgttattc
      721 gcgaagttaa tgaaccgcaa acggtactcg ccatggtcag tatgggtatt ggtatcacat
      781 tgatagcgga cagctacgcg cagatgaact ggcccggtgt catattccgt ccgctcaaag
      841 aacgcatccc ggcagactta tatattgttt atgaaacgca gcaggtgacg cccgcgctgg
      901 ttaaactgct ggcggcgttg gcgcagtaga tgagaggcac gggaactgcc ggacatagtt
      961 ggtctgataa gcgaagcgca tcaggcaatt tcgcaaattg cagatagcaa aaaaacgcct
     1021 ttagggcgct tttttacatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
     1081 aaagtcaact gctctaccaa ctgagctaac gacccgaagt ggtgggtgat gacgggatcg
     1141 aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat cacccacttc
     1201 ggtacttcac attgttacaa gaaatctagc tggcgagaaa gtggtgggtg atgacgggat
     1261 cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta atcacccact
     1321 tctcaatttc ttgctacacg gcggagacta cataaagtag ttggtgggtg atgacgggat
     1381 cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta atcacccccg
     1441 ctgtgtggag tcgcattata gggagagttc aaaatgagtc aacgcatttt ctaaagaaat
     1501 tgttcgttcg tcgtaaattt aagcaagatg atcgcaaaac agaccgtgtt gcgcaatttg
     1561 tcaacgaaaa caataatgcg taaggttgca acccgaacta cattgaggaa tcaggcggga
     1621 gtgatagaat atcgcccact caatttttcc aggatttgcc ggttgtcggc atctttctaa
     1681 acgtaaggcc atttcatgaa aatcaaaact cgcttcgcgc caagcccaac aggctatctg
     1741 cacgttggcg gcgcgcgtac tgctctttac tcctggcttt ttgcacgtaa ccacggcggt
     1801 gagttcgtgc tgcgtattga agacaccgat cttgagcgtt ccacgccgga agctatcgaa
     1861 gccattatgg atggcatgaa ctggctgagc ctggagtggg atgaaggtcc gtactaccag
     1921 accaaacgtt ttgatcgcta caacgcggtg atcgatcaga tgctggaaga gggcacagct
     1981 tataaatgct attgctctaa agagcgcctg gaagcgctgc gcgaagagca aatggcgaaa
     2041 ggtgagaagc cgcgttatga cggtcgctgc cgccacagtc atgagcatca cgctgatgat
     2101 gagccgtgtg tcgtacgttt tgctaacccg caggaaggtt ctgttgtttt tgacgatcag
     2161 atccgtggtc cgatcgagtt cagcaaccag gagctggatg atctgattat ccgccgtacc
     2221 gatggttccc caacctataa cttctgtgtg gttgtcgatg actgggatat ggaaatcacc
     2281 cacgttatcc gtggcgaaga ccatatcaac aacacaccgc gccagattaa cattcttaag
     2341 gctctgaaag cgccggtacc ggtttatgcg cacgtttcta tgatcaacgg cgatgacggt
     2401 aaaaaactgt ccaaacgtca cggggcggtc agcgtaatgc agtatcgtga tgatggttat
     2461 ctgccagaag cactgctgaa ctatctggtg cgtctgggct ggtcccacgg cgatcaggaa
     2521 atcttcactc gtgaagagat gattaaatac ttcactctga atgccgtcag caaatctgcc
     2581 agtgcgttca acaccgacaa gctgctgtgg ctgaaccatc actacattaa cgcgttgccg
     2641 ccggagtatg ttgctactca cttacagtgg cacattgagc aggaaaatat cgatacccgt
     2701 aacggcccgc agctggctga tctggtgaaa ctgttgggcg aacgctgcaa gacgctgaaa
     2761 gagatggcac agagctgccg ttatttctac gaagattttg ctgagttcga tgccgacgcc
     2821 gcgaaaaaac atctgcgtcc ggtagcgcgc cagccgctgg aagtggttcg tgacaaactg
     2881 actgcgatta ctgactggac cgctgaaaac gttcatcacg ctattcaggc gacggcggat
     2941 gagctggaag tgggtatggg taaagttggt atgccgctgc gtgtcgccgt aaccggtgcg
     3001 gggcagtctc cggcgctgga tgttaccgtt cacgcaatcg gtaagacccg cagtatcgag
     3061 cgtatcaaca aagcgctggc ttttattgct gaacgcgaaa atcagcagta attagcgcat
     3121 aaaagataaa cggcaggaga gatacccctg ccgtttttta tttatgctga ttcatctatg
     3181 tcgagacgag caagaaaatt acgaattcct tcgcgtgaca aaaattgcgc aactttttct
     3241 tgctcactgt gagtcatgtt gtctattgcg ctgatgattt gccgataagc aggagtgcga
     3301 atacccggtg cgtattcggc aaggggttct tcggcttcca gaagcatcgg tgtgttatgg
     3361 aaggctggcg tgttctgaat aaactcgcaa acttgcgtat cgaccagaat aagtctggca
     3421 cgaccgcctt tcacgccggg aaatttttcc gttttccagc ctttttctct gatccaacgg
     3481 ttaacagttt gtttagccac accgaggcat tcagccagtt cttcggtggt cattttattg
     3541 cgtaatcttt tcatattatt tgctatcgcg aatccctaag cgttgtaaca atccgataat
     3601 cccttcccgc agaagcaagg atgtaaactg tttttgttca actggcgtca tctctttcgc
     3661 cagtgtcacc agcaacactt caagcggcgc atcaccagaa agggctggcg cttctccctg
     3721 accttctgga cgttcagcat tgcgaatata ttcacgaacc tgttcattga cgtgaaccag
     3781 tcgagccttg ccaccctgga cgcctggttt tggtgacgtt gtccagcctt ccttgcgtac
     3841 ccatttatta atggtctggc ggctatagcc ggtcagtctg gcaagttcat ctggcgtcat
     3901 cctctccttg aacataacaa ttccctgaat gtatgtgggt ctattagtag ttcattttat
     3961 aacaccactt tacaggaaac cgtgacatga ataactactg aatgcgagcg gttttccaaa
     4021 attgttcatt tgcctgattt ttcagcgatt gaaactacac gtggcaattt gccgttgaca
     4081 cattcgggcg gaattcatat gatgccgccc gtcaactcga caagctttac gtgatggggc
     4141 tatagctcag ctgggagagc gcttgcatgg catgcaagag gtcagcggtt cgatcccgct
     4201 tagctccacc aaattttgca cccagcaaat ttggtacgta aacgactcgt ggggctatag
     4261 ctcagctggg agagcgcttg catggcatgc aagaggtcag cggttcgatc ccgcttagct
     4321 ccaccaaatt tcaaaccctc gctgcaaagc gggggttttt tgtctctgcc ttttgccgct
     4381 tttgtaatac agtctacatc ctggcagtgc cgtgtggtga atgcatcact gactgcaaaa
     4441 acagcaacag gctggcccct gtttgcttcg cgatgcgaat aaacttatta tttgtgtgcc
     4501 tgaaaacccc gatcagtgag agtagtgtac tcatgtttgt ggagcataac ctgataaaaa
     4561 atatcaagat attcacacta gcgtttacgc tcaccgtggt acttattcag ctatcccgtt
     4621 taatttcgcc acttgctgtt atccattcca attatatctt tctggcgtgg atgccactgt
     4681 gcgtaatgct gtcaatcttg tttatctttg gctggcgtgg tgtcgttccc attttatgtg
     4741 ggatgttttg caccaatctg tggaaccttc atctcacttt tttacagacc gccgtcatga
     4801 tcggcagcca ggcgtttgca gtcctgtgtg cctgtgcaat attacgctgg caattgggga
     4861 cgcgttggcg ttatggattg accagccgat acgtctggca acgtctgttc tggcttggtt
     4921 tggtggcgcc gattggcatc aaatgcagca tgtatcttgt gggaaatttc tttgattttc
     4981 cgctaaagat atctaccttt ttcggcgatg cggatgccat tttcacggtc gttgatttgc
     5041 taagcctttt caccgcagtg ctgatttaca acatgctttt ttactatctc acccgcatga
     5101 ttgtaagtcc ccactttgca cagatattgt ggcgtaggga tatcgctccg tcgttgagca
     5161 aagagaaacg cgcatttacc ttaagctggc tggcagctct tagcgtgctg ctacttctga
     5221 tgtgcacacc gtatgaaaat gactttattg ccggttactt ggtacctgtt ttctttatca
     5281 tctttaccct cggtgtcggt aagcttcgct atccgttttt aaatctcacc tgggctgttt
     5341 caacgctttg cctgctgaat tacaaccaga actttttgca aggggtacta accgaatatt
     5401 cgctggcatt tattctcgca gtactgattt cctttagtgt ttgtctgctc tatatggtgc
     5461 gtatttatca tcgtagtgaa tggcttaacc gccgctggca tttgcaggcg ctgaccgatc
     5521 cgttaacgct cctacccaac tttcgtgcgt tggaacaagc accggagcaa gaggcgggca
     5581 agagtttttg ctgcctgcgc attgataatc ttgagtttat gagtcgtcat tacggcttaa
     5641 tgatgcgcgt tcactgtatc cgctcaattt accgtacgtt gctgccgttg atgcaggaaa
     5701 acgaaaagtt gtatcaattg ccgggtagtg aactgctgtt agtgctgagc gggccggaaa
     5761 cggaagggcg actccagcat atggttaaca tcctgaatag tcggcaaatt cactggaaca
     5821 ataccgggct ggatatgggc tatggtgctg cctgggggca ttttgatgga aatcaggaaa
     5881 ccctgcaacc cttgttgggg cagttaagct ggctggcaga gcaatcctgc gcacatcatc
     5941 atgtgctggc gctggatagc agagaggaga tggtttccgg gcaaaccact aaacaggtgc
     6001 tattgctgaa taccattcgc acggcgttag atcagggtga tttgctgctc tacgcccagc
     6061 caattcgcaa caaagagggt gaaggttatg atgagatcct cgcgcgactg aagtatgacg
     6121 gcggcattat gaccccggat aagtttctgc cccttattgc tcagtttaac cttagcgcgc
     6181 gttttgattt gcaagtgctg gaatccttgt tgaagtggct ggcaacacac ccttgcgaca
     6241 aaaaaggtcc acgattttca gtcaatttaa tgccgctcac gctgctgcaa aagaatattg
     6301 ccgggcggat tattcgtctg tttaagcgtt attacatctc cccgcaggcg gtcattcttg
     6361 agatcaccga ggagcaggcg ttttctaacg cagaaagcag catgtacaac atcgagcagc
     6421 tgcataagtt tggtttccgg attgcgattg atgactttgg caccggatat gccaactacg
     6481 aacggttaaa gcgtttgcag gctgatatca tcaaaattga tggcgtcttt gtgaaagata
     6541 tcgtcacgaa cacgctggat gcgatgattg tgagatcaat taccgatctg gcgaaagcga
     6601 agtcattgag tgtggtcgcg gagtttgtcg agacgcaaca gcagcaggcg ctattgcata
     6661 agctcggggt gcaatatctg caagggtatt tgattggtcg cccgcagcca ttagccgatt
     6721 aacaggcgta aaaaagccgg ggaattatcc ccggcttttt tatgtatggt cttacagcac
     6781 cagtgctgcg attgacgcag acagcacact caccagggta gagccgtaaa ccagtttcag
     6841 accgaagcga gaaaccacgt taccttgctc ttcattcagg cctttaaccg cacccgcgat
     6901 aatcccgatt gaagagaagt tagcgaagga aaccaggaac acagagatga tgccttccgc
     6961 acgcggagag agcgtggaag caattttctg cagatccatc atcgcaacga attcgttgga
     7021 aaccagtttg gtcgccatga tactgcccac ttgcagtgct tcactggaag gaacacccat
     7081 cacccatgca atcggataga agatgtaacc caggatgccc tggaaggaga tgctgtagcc
     7141 aaaccagcca gtcacggtgg caaacagtgc gttcagcgcg gcgatcaggg cgataaagcc
     7201 gatcagcatc gccgcaacga taatggcaac cttgaaacct gccagaatgt attcacccag
     7261 catttcgaag aagctctgac cttcgtgcag gttggacatc tgaatatttt cttcactggc
     7321 atcaacacgg taagggttga tcagcgacag cacgataaag gtgctgaaca tgttcagtac
     7381 cagcgccgca acgacgtatt tcggctccag catggtcatg tatgcaccaa cgatggacat
     7441 cgacacggtg gacatcgccg ttgctgccat ggtgtacata cgattgcggg agattttgcc
     7501 gaggatatct ttataggcaa taaagttttc agactgaccc agaatcaggg agctgacggc
     7561 gttaaaggat tccagtttgc ccatgccgtt gactttggag agcaggaaac caattgcgcg
     7621 gataatcacc ggcaacacgc gaatgtgctg gagaataccg atcagtgcag agataaagac
     7681 gattgggcac agcactttca ggaagaagaa tgccaggcct tgatcattca tgctaccaaa
     7741 gacgaagtta gtcccttcgt tggcaaaacc gagcagtttt tcgaacattt cggagaagcc
     7801 tttcacgaag cccagaccaa cgtcggagtt caggaagaac cacgccagta acacttcgat
     7861 aacaagcagt tgaataacat aacggatacg aatttttttg cggtcgctgc ttaccagcag
     7921 tgcgagaatc gcaacaacgg caagtgccag tacaaaatga aggacgcggt ccatatttgc
     7981 tccaaatatg aggcaggtta aatttccgtg cacattctat gtaacatggt taaggaaaac
     8041 gagatctgac acacactata atgacgtcct gtgacaagct tcaaaaatag tgatctgtca
     8101 tacatttctg ctatgttgtg tatggaagcg gaaggttatg taaatgtgct aatattaaac
     8161 ataaccataa cattcgagtc atctatcttt tcgtagtcat aatcctggtc tatcagagaa
     8221 atcatcacaa cccatttaaa tgaatttgat aatcattctc atttaacgta gcatgaaaca
     8281 tagcaaaggc tatgttttag aggcacaaga tgacgaacta tcgcgttgag agtagcagcg
     8341 gacgggcggc gcgcaagatg aggctcgcat taatgggacc tgcgttcatt gcggcgattg
     8401 gttatatcga tcccggtaac tttgcgacca atattcaggc gggtgccagc ttcggctatc
     8461 agctactgtg ggttgtcgtt tgggctaacc tgatggcgat gctgattcaa atcctctctg
     8521 ccaaactagg gattgccacc ggtaaaaatc tggcggagca gattcgcgat cactatccgc
     8581 gtcccgtagt gtggttctat tgggttcagg cagaaattat tgcgatggca accgacctgg
     8641 cggaatttat tggtgcggcg atcggtttta aactcattct tggtgtttcg ttgttgcagg
     8701 gcgcggtgct gacggggatc gcgactttcc ttattttaat gctgcaacgt cgcgggcaaa
     8761 aaccgctgga gaaagtgatt ggcgggttac tgttgtttgt tgccgctgct tacattgtcg
     8821 agttgatttt ctcccagcct aacctggcgc agctgggtaa aggaatggtg atcccgagtt
     8881 tacctacttc ggaggcggtc ttcctggccg cgggcgtgtt aggggcgacg attatgccgc
     8941 atgtgattta tttgcactct tcgctcactc agcatttaca tggcggttcg cgtcaacaac
     9001 gttattccgc caccaaatgg gatgtggcta tcgccatgac gattgccggt tttgttaatc
     9061 tggcgatgat ggctacagct gcggcggcgt tccacttttc tggtcatact ggtgttgccg
     9121 atcttgatga ggcttatctg acgctgcaac cgctgttaag ccatgctgcg gcaacggtct
     9181 ttggattaag cctggttgct gccgggttgt cctcaacggt ggtggggacg ctggcggggc
     9241 aggtagtgat gcagggcttc attcgctttc atatcccgct gtgggtgcgt cgtactgtca
     9301 ccatgctgcc gtcatttatt gtcattctga tgggattaga tccgacacgg attctggtta
     9361 tgagtcaggt gctgttaagt tttggtatcg ccctggcgct ggttccactg ttgattttca
     9421 ccagtgacag caagttgatg ggcgatctgg tgaacagcaa acgcgtaaaa cagacaggct
     9481 gggtgattgt ggtgctggta gtggcgctga atatctggtt actggtagga acggcactgg
     9541 gattgtagtt gaatgagcgt cgcatttggc accattggcg gatgcggcgt aaatgcttta
     9601 tccgccctac gcgttaaaga gctggtttgt aggcctgata agacgcgcta gcgtcgcatc
     9661 aggcattgtg ctccattggc gaatgtgttt aatggcggtg tccatgtccg cggccttttc
     9721 cacgatgatc gtcacgatcg cgccagcctt cacgatagcc acgctcatac gctttacgct
     9781 tatcccagcc acgatggtag ccattatcat gacgccacca gcggttttta cgccattcat
     9841 aattgcggtg ccagtagtca cggtcgcgcc agtgaccacc gtcccagtaa ttaccgtaat
     9901 gatcgcgatc gccaatttgt aatttgattg atggcagtag ggtgatttca cctgcgtttg
     9961 ccgcaagcgg ggtaaatgcc atcagggcgg ccgccagaaa cagtgacctg aacattgtta
    10021 ttctccttca cgatcgaagc cgtcagcggc ctgttaacgc aatattacgg ggaggtaaag
    10081 cgccgtatca tcgccataac tcttaaatca taaaggagag cattttttgc taaacgttat
    10141 cgtggggtgg ctgttgcttc ggacgattga ccatcatcat cggcttacct gctaataaca
    10201 gccatgccgg gagcatgacg atacagagca gcggtagcaa tgtggtattg ggtacaacga
    10261 ctgccgccat aaaaagactt aaccagccat cacgtgttac caccagcacg atgccgagaa
    10321 tagcgcagga aacggttatt gccgctggaa cggcgggtac gtgctcatgc agcatcaacc
    10381 ctaatgccac gccgacaaac actgccggga agattcgccc accgcgaaag ccactggcgg
    10441 cagcaacgac cagggcggca agttttatca ccgccagcaa aaagtaatcg ctggtgctga
    10501 aagcctgatt tgccaccatc tgctgcatct catccagccc tttaaacagc gaaaccggtc
    10561 caccaataac ccccagaata ccgagaataa atccgccaat acccagcacg aacaccggat
    10621 ttttcatttg atgcatcatc gcgtgcaacc gtggtaagca ccatacggcg accatccctg
    10681 ctgcgatggc aatcgccgcg acaattgcac cgctgagaat atcggtcatt tccatctgtc
    10741 cgtaatgcgc aatgggcagt gaaaaatgag gatggaaaaa taatccggta gtaagtgcac
    10801 cagctgctgc cgccattaac ggcgcaaaga gacgatccca tagcggaact tcattactgc
    10861 catttaaggt ttgcgaaaat atcaatgccg ccgcaacagg tgtgccaaac agcgcgccga
    10921 tggttccggc agaggctaaa atagtccact ccattcggtt gacgcgcggt aacagacgag
    10981 cgccaatggc gacggcaagg gcgatattga cggtcatgat cggatgttcc ggccccaggc
    11041 tgacgccgcc agcaagaccg agaattaatg ctacgataag tcctggtagc gcagagggcg
    11101 gaaccggtgc gccgatcagc ggttcacagg cggggtctgg tccggcatga ccctggctga
    11161 aacggataac caaccccacc gcaatacccg ttagcgttaa tacaccgatg atccagagag
    11221 gtgaatcctg ggctatcccc agagttcccg gcagtcgttg ccagagcaaa ttctgtaata
    11281 ccgaggcgat tttcatcacc acaatcagaa taagactgga cgcaatccca attgccacgg
    11341 cggggagcga taataacaac atggttctgg ctcgcggatg gagcatgttc tattccttat
    11401 gcggggtcag atacttagtt tgcccagctt gcaaaaaggc atcgctgcaa ttggtgctga
    11461 aacgataaag taattgtgtg acccagatcg atatttacag ggagcctgcc tttccggcgt
    11521 tgttgttatg cccccaggta tttacagtgt gagaaagaat tattttgact ttagcggagc
    11581 agttgaagaa tgacaaagta tgcattagtc ggtgatgtgg gcggcaccaa cgcacgtctt
    11641 gctctgtgtg atattgccag tggtgaaatc tcgcaggcta agacctattc agggcttgat
    11701 taccccagcc tcgaagcggt cattcgcgtt tatcttgaag aacataaggt cgaggtgaaa
    11761 gacggctgta ttgccatcgc ttgcccaatt accggtgact gggtggcaat gaccaaccat
    11821 acctgggcgt tctcaattgc cgaaatgaaa aagaatctcg gttttagcca tctggaaatt
    11881 attaacgatt ttactgctgt gtcgatggcg atcccgatgc tgaaaaaaga gcatctgatt
    11941 cagtttggtg gcgcagaacc ggtagaaggt aagcctattg cggtttacgg tgccggaaca
    12001 gggcttgggg ttgcgcatct ggtccatgtc gataagcgtt gggtaagctt gccaggagaa
    12061 ggcggtcacg ttgattttgc gccgaatagt gaagaagagg gcattatcct cgaaatattg
    12121 cgtgcggaaa ttggtcatgt ttcggcggaa cgcgtgcttt ctggccctgg gctggtgaat
    12181 ttgtatcgcg caattgttaa agccgacaac cgcctgccag aaaatctcaa gccaaaagat
    12241 attaccgaac gcgcgctggc tgacagctgc accgattgcc gccgcgcgtt gtcgctgttt
    12301 tgcgtcatta tgggccgttt tggcggcaat ctggcgctca atctcgggac atttggcggc
    12361 gtgtttattg cgggtggtat cgtgccgcgc tttcttgagt tcttcaaagc ctctggtttc
    12421 cgcgccgcat ttgaagataa agggcgcttt aaggaatatg tccatgatat tccggtatat
    12481 ctcatcgtcc atgacaatcc gggtcttctc ggttccggtg cacatttacg ccagacctta
    12541 ggtcacattc tctaaatcct tcctgttata tcgggaggta actctcccga taatctttta
    12601 aatcatacag ttaattaaat ttttctttgt gtcccctcac aaggccgacc tgcgtcacac
    12661 ttccgtacag cgggattaat tctccagtaa atgcattatt tgtctggtaa cggcgatttg
    12721 ttttgcacgt tcataatttc actcgtcaac tatcactgaa cgaggaatta acgatgagta
    12781 agaaactgat tgccttatgt gcctgcccaa tgggcctggc tcacactttt atggccgctc
    12841 aggcgctgga agaagcggcg gtagaagccg gttatgaagt gaaaattgaa acccagggcg
    12901 cggacggtat ccagaatcgc ctgacggcgc aggatatcgc cgaagcgacc atcatcatcc
    12961 actccgtggc agttaccccg gaagataacg agcgtttcga atcacgcgac gtttatgaaa
    13021 tcactttgca ggacgcaatt aaaaacgctg cgggaatcat caaagaaatc gaagagatga
    13081 ttgcctctga acaacagtaa tattcgacag ggataattgt tatggccatt aaaaaacgca
    13141 gtgcaaccgt tgtgcatggc gcatccggtg cggcagcggc agttaagaat ccgcaggcct
    13201 ctaaatccag cttctggggt gaactcccgc agcatgtgat gtcagggatt tcacgcatgg
    13261 tgccgacctt aattatgggc ggtgtgatcc tcgctttcag tcagctgatt gcttatagct
    13321 ggcttaaaat tcctgctgat atcggcatca tggatgctct taatagcggg aaattctccg
    13381 gtttcgacct ctccttactt aaatttgcct ggctgtcgca gtccttcggc ggcgtgttgt
    13441 ttggttttgc aattccgatg tttgctgctt ttgtggcgaa ctctatcggt ggcaaactgg
    13501 cgttcccggc tggttttatc ggcggcttga tgtctaccca gccgacgcaa ctgctgaact
    13561 tcgatcccag cactatgcaa tgggcgacct cttcgccggt gccgtccacc ttcattggtg
    13621 cgctgattat ttctatcgtt gccggttacc ttgtgaaatg gatgaaccag aaaatccagt
    13681 taccggattt cctgttagcg ttcaaaacca catttttgct accgattctt tccgccattt
    13741 ttgtcatgct ggcgatgtac tacgtcatca ccccctttgg tggctggatc aacggcggta
    13801 tccgtaccgt gctgaccgct gcaggtgaga agggcgcact gatgtacgca atggggatcg
    13861 ccgcggcgac agcaatcgac cttggtggcc cgatcaacaa agcggcaggt ttcgttgcct
    13921 tcagctttac cactgaccac gtattaccgg tcactgcacg ttctatcgct atcgttattc
    13981 cgccgattgg tctgggtctg gcgaccatta ttgaccgtcg tttaaccggc aaacgcttgt
    14041 tcaacgccca gctttatccg cagggtaaaa ccgccatgtt cctggcgttt atggggatca
    14101 gtgagggtgc gattccgttt gcactggaaa gccctatcac cgccattccg tcgtatatgg
    14161 tcggcgcgat tgtcggctca accgccgctg tctggctggg cgcagtgcaa tggttcccgg
    14221 agtccgctat ctgggcatgg ccgctggtca ctaacctcgg cgtctacatg gcggggatcg
    14281 cgctgggggc agtcataact gcgctgatgg tggtgttcct gcgtctgatg atgttccgta
    14341 aaggcaaatt gttaatcgat agcctgtaag aaggacaatc ctgatgacat tactcgcttc
    14401 gctgcgcgac tggcttaagg cgcaacaact ggatgcggtg cttctctcct cacggcagaa
    14461 caaacagccg catctgggga tctccaccgg atcaggttat gtggtgatta gccgtgaaag
    14521 tgcgcacatt ctggtggatt cgcgctatta cgcggatgta gaagcccgca cgcaaggtta
    14581 ccagctgcat ttgctcgacg cgatgcacac gcttgccacc atcgtcagac aaatcattgc
    14641 cgatgagcag ttgcaaacgc tcggttttga aggccagcag gtgagttggg aaaccgcgca
    14701 ccgctggcag tccgaactca atgcgaaact ggtgagcgcc acgccggatg tgctgcggca
    14761 aatcaaaacg ccagaagagg tggagaaaat ccgccttgcc tgcgggattg ccgatcgtgg
    14821 cgcagagcat attcgccgct ttattcaggc gggaatgagc gaatgcgaga tagccgctga
    14881 actggagtgg tttatgcgcc agcagggcgc agaaaaagcc tcttttgaca ccattgtcgc
    14941 cagcggctgg cgtggggcgc tgccgcacgg caaagccagc gacaagattg ttgcagcggg
    15001 cgagtttgtc actctcgatt tcggtgcgct gtatcagggc tactgctctg atatgacgcg
    15061 caccttgctg gtgaatggcg aaggggtgag cgccgaatct catccgctgt ttaacgtcta
    15121 tcagattgtc ctgcaggcac agctcgcggc aatctctgta attcgccccg gcgtgcgctg
    15181 ccagcaggtt gacgatgccg cgcgccgcgt gattaccgag gccggttatg gcaagttttt
    15241 cggccataac accggtcatg ctatcggtat tgaagtccat gaagaaccgc gtttttcacc
    15301 gcgggacacc acgacgctac agccaggtat gttactgacc gtggagccag ggatttattt
    15361 gccagggcaa gggggcgtgc gcatcgaaga tgtcgtgctg gtcaccccgc aaggcgcaga
    15421 agtgctctac gccgtgccga aaacagtgtt gctcacggga gaggcataat ggatttatcg
    15481 ctcttaaaag cgttgagcga ggcagatgcg atcgcctcct cagaacagga agtacggcag
    15541 atcctgcggg aagaagcgga tcgcctgcaa aaagaagtgc gatttgacgg cctgggatcg
    15601 gtgctgatcc gactcaatga gtcgacaggt ccgaaggtga tgatctgtgc gcatatggac
    15661 gaagtgggat ttatggtgcg cagcatctcc cgcgaagggg cgatcgatgt gttgccggtt
    15721 ggcaatgtac gcatggctgc ccgccagctt cagtcggtgc gtatcactac ccgtgaagag
    15781 tgcaaaattc caggcctgct tgacagcgac cggcagggga atgacgtcag cgccatgcgc
    15841 gtggacattg gcgcgcgctc ctgtgacgaa gtgatgcagg caggaattcg tccaggcgat
    15901 cgcgtcacct tcgataccac ttttcaggtt ctccctcacc agcgggtgat ggggaaagcc
    15961 tttgatgacc gcctcggttg ctatctgctg gtgacgttac tgcgtgagct gcacgacgcc
    16021 gaactgcctg cggaagtgtg gctggtggca agttccagcg aagaggtggg attacgcggc
    16081 gggcaaactg ccacccgcgc ggtgtcgccg gatgtcgcca ttgtgcttga tactgcctgc
    16141 tgggcgaaaa actttgatta tggcgcagct aaccatcgcc agattggtaa cgggccgatg
    16201 ctggtgttaa gcgacaagtc actgattgcg ccgccaaaac ttaccgcctg gatcgaaacc
    16261 gtggcggcag aaattggcgt accgttgcag tcagatatgt tcagcaatgg cggcacggac
    16321 ggtggggcgg tgcacttaac cggcactggc gtacccacag tggtgatggg gcctgcaacc
    16381 cgccacggac attgcgccgc atcgattgct gactgccgtg acattttgca gatggagcaa
    16441 cttttatctg cccttattca acgtcttacg cgtgagacgg ttgttcaact gacggatttc
    16501 agatgatctc ctgattaacc cggagcggtt atgttaacga ttcaatttct ctgtcctttg
    16561 cctaacggtc tacatgctcg tccggcgtgg gaacttaaag agcagtgcag ccagtggcaa
    16621 agcgaaatca cttttattaa ccatcgccag aacgcaaagg ccgatgcgaa aagctcgctg
    16681 gcgctgattg gcactggcac tttatttaat gacagttgca gcctgaacat tagcggtagc
    16741 gatgaagagc aggcgcggcg agtgctggaa gagtatatcc aggtgcgctt tatcgacagc
    16801 gatagcgtcc agccgacgct ggcagaactg acggcgcatc cgctgccgcg ttcattaagc
    16861 cgcctgaacc cggatttact gtacggcaac gtgctggcaa gcggcgttgg cgtgggtaca
    16921 ctgaccctgt tacagagcga cagcctcgac agttatcggg tgatccctgc gagtgcgcaa
    16981 gattccaccc tactggagca cagtctggca acgcttgccg agcaactgaa ccagcaattg
    17041 cgtgagcgtg acggcgaaag caaaactatc ctcagcgccc atttgtcgct gattcaggat
    17101 gatgaatttg caggcaatat ccgtcacctg atggcagaac agcatcaggg gctgggggcg
    17161 gcgatcatca gcaatatgga gcaggtttgt gccaaacttt ctgcttctgc cagcgattat
    17221 ctgcgcgagc gggtcagcga cattcgcgat atcagcgaac agttgctgca tatcacctgg
    17281 ccggaactaa aaccgcgcaa taatctggtg cttgaaaaac tgaccattct ggtggctgaa
    17341 gatttaaccc caagccagtt tttgagcctc gatttaaaaa atcttgcggg catgattctg
    17401 gagaaaaccg ggcgcacctc gcataccctg atcctggccc gcgcttcggc gatcccggta
    17461 ctgagcggac tgccgctgga tgcgattgcc cgttatgccg ggcaacctgc agtgcttgac
    17521 gcccagtgcg gcgtgctggc gattaacccg aatgacgcgg tgagcggtta ttatcaggtc
    17581 gcgcagacgc tggcggataa acgccaaaaa caacaggcgc aagctgccgc gcagctggcc
    17641 tattcccgtg ataacaaacg tattgatatt gcggcgaata ttggcaccgc tctggaagcc
    17701 ccaggcgcgt ttgctaacgg cgcggaaggc gttgggctgt ttcgtaccga aatgctctat
    17761 atggatcgcg acagcgcgcc ggacgagcag gaacagtttg aagcgtacca gcaggtgcta
    17821 ttggcggcgg gcgacaagcc gattatcttc cgcacgatgg acatcggtgg cgataaaagc
    17881 attccttacc tcaacattcc ccaggaagag aacccgttcc tcggctatcg cgcggtacgt
    17941 atttacccgg aatttgctgg cctgttccgc actcaattgc gggcgatttt gcgcgctgcc
    18001 agtttcggca acgcccagtt gatgatccca atggtccaca gtctcgatca gatcttatgg
    18061 gtgaagggtg agatccaaaa agcgatcgtt gagcttaagc gcgatggcct gcgtcatgca
    18121 gagacgatta cgctgggtat catggtggaa gtgccgtcgg tgtgctacat catcgaccac
    18181 ttctgcgatg aggtcgattt cttcagtatc ggttctaacg atatgaccca gtatctgtat
    18241 gcagtcgatc gtaataaccc gcgcgtatcg ccgctgtata acccgattac gccatcgttc
    18301 ctgcgcatgt tgcagcagat tgttaccgct gcgcatcagc gcggcaaatg ggtaggtatt
    18361 tgcggtgaac tgggcggtga aagtcgttat ctgccgctac tgctagggct ggggctggat
    18421 gagctaagca tgagtagccc gcgtattccg gcagtgaaaa gccagcttcg tcaactggat
    18481 agcgaggcgt gtcgggaact ggcgcgtcag gcgtgcgagt gccgcagcgc gcaggaaatt
    18541 gaagcgttac ttaccgcgtt tgcgccggaa gaagacgttc gcccactgct ggcactagag
    18601 aatatctttg ttgatcagtc ctttagtaat aaagagcagg cgatccagtt cctgtgcggt
    18661 aacctcggtg ttaacgggcg tactgaacat ccgttcgagc tggaagaaga tgtctggcag
    18721 cgggaagaga ttgttactac tggcgttggt tttggcgtgg cgatcccgca caccaaatca
    18781 cagtggatcc gtcattccag tatcagcatt gcccggttgg tgaaaccggt tgactggcag
    18841 tcagaaatgg gcgaagtcga actggtgatc atgctgacgc tgggcgctaa cgaagggatg
    18901 aatcatgtga aagtcttctc gcagctggcg cgtaaactgg tgaataaaaa cttccgccag
    18961 tcgctgtttg ccgcgcaaga tgcacaaagt attctgacgc tgctggaaac ggaattaacc
    19021 ttctgacgtt agccctgaaa acgtgcgcta tactctcccg gcgtcagacc aaactgacgc
    19081 cggaaaacgc gacaaaaata gtcgctatcc ggaaacccgc aacgctgcgc cacttcatga
    19141 atcgacagat gatatttttg caaaatcatc ctcgcttttg ccattcgtac ccagcgaaca
    19201 tactcgataa aacgcattgt tccatgctga gcaaacagtt ttgacagatg atttggcgtg
    19261 atattaaaaa actgcgccac gctctcgcgg gtgagcagct gggcataatt atcctgaatc
    19321 cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt gcgaattgcc cggcggcata
    19381 ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca gtggctgaat gatattctgc
    19441 tccagcggcg aacggctaag atgggtgagg gcggtaagca tcgcttcacc ttcaccgcgt
    19501 tgcaggctgg gaagctggat ctggcgagca ggatgtaaca acgacgtggt gcggctatcg
    19561 taaaacgata acccaagcca ggtcggtgcg aacaccaggc ttaacagcag caccggtttg
    19621 ttgttgaccg gtaaattagc cgcgcgtgct ggaataaaca gcatttctcc ttccaccata
    19681 cgttgctgct ttgcttccag cagattgccg tattcgccgc gtagcacgat atccagacgg
    19741 ggaaaatcaa tacataaact gcccactggc aatgaggtag gctggctggc aaaccagacg
    19801 cgccccagta gttgcgggtt aagcaccagg ccgctgaaca gatcggcgaa aaattgttga
    19861 tcggcgggca agcctggtgc tttcatgaaa gttcccgatt aaagatgcat taactggcgg
    19921 aattctttaa ctttgctgca gctgaccggc acttcaaaat ccagatcttt cagccgcaga
    19981 atataggtgt tattaaacca cggttcgatc tcgcggattt tgttcagatt gacacaaaat
    20041 gagcgatggc agcggaaaaa atgcgacggc ggcagcttgc tgcaaaactc ggtaatgttc
    20101 atcggcatta cgtaggattc acgccgcgta tagacaaagg tcattttctc atgcgcttcg
    20161 gcgtaataga tatcgttaat gggcgtgacg atgattcgct catctttcac cagattaatg
    20221 gtgtcattct cacgcgttac cgtcgcggca ggcgtactgc tggtctgctg ttgttgccag
    20281 gctgcttcca gtttttgcag catcccggta atgcgtgact cctgatacgg tttgagaatg
    20341 tagtcaaacg cctccagttc aaacgcttct accgcatgtt ctttccacgc ggtgataaac
    20401 acaataaacg gtttatgggc gaactggctg atgttttgtg ccagcaacac accatccagc
    20461 gacggaatat tgatatccag aaaaatggcg tcgacgcggt tatgctgcaa aaacttcaac
    20521 acgtccagac cgtcgtcaaa ggtgccgaca atctccatct ggctgtgctc tttaattagc
    20581 cagctcagtt cctgttgtgc caggaattcg tcttcaacaa tgatgacttt cacaatatca
    20641 ctccggctca aagcaataag gtagcctgtg aggcgactgg ggtgcgttgg ttaggaatgt
    20701 aaaacgcaat ttccgttcct ggttccaggc ggcggatatg cagcccctcg ccatacaata
    20761 acttcacgcg atgatggaca ttcagcaggc caattttatt gcccggcatt tcgttcgctt
    20821 cgacccgctc aatcaccttt ggatcgatgc cgtgcccggt atctcgcacc gcaatgcgta
    20881 cccgattacc gcactcagca acgctgatgg tgacaacgcc tttacctttg caaggctgga
    20941 taccgtggac aatggcgttt tccaccaacg gctggatcag caggctggga atgcagcaat
    21001 tcacctcttc atcaatatca tagataaccg tcagcttgtc gccaaagcgg gcctgctcaa
    21061 tggcaatata gtctttaatt tgatacagct cttttttgat atcgatttgc tcatcgtctt
    21121 ttaattcaat gttatagcgc agatagcgcg acaaattaaa gatcagctgg cgagcagtat
    21181 ccggattcag acggattgac gatgaaatag cgttcaaagc gttaaacagg aaatggggat
    21241 taattttgct ttgcagggcg cgaagctctg ccttatttgc catttcacgt agctgctcgg
    21301 cgcgtgacac ctcaagttgc gtggagatga tttgtgacag accgaccgcc atttcctgta
    21361 atgacgaggt tatctgatgc gcgtggcagt agtaaatttt cagcgttccg gtcacaacgc
    21421 ctttctccca caatgggatc accagcatgg aatgaatctc tggtgtgcgg tgggcttcat
    21481 cattgttttt aatgatgatt ttcccgtaat tcatcgcctg acgggtagtc gggctaatga
    21541 agtcatcgcc attctgatag ttatgttcac ccacgccaac gtaggccagc acatgatcgg
    21601 tattagtcat cgccaccgca tcggcgtgaa tgtcatcgcg gataatttcg cagaccttgc
    21661 gtaatgactc gctattgaca tggcgaaaca gcggcagcgt tttgttggcg atatccagcg
    21721 ccagcttggc ctgccgcgcg gcgctggcct ctttttctcc ctcaacactt tgtaccagca
    21781 acacaataaa gccgatacag acgctgccga ggatcattgg aatcccgatt ttagagacga
    21841 tatcgatccc caatgcggta gttggtgccc agacaatcac cagaatcatg gtcaacgttt
    21901 cacacaacat gccgccgaga ataccgacgc gccagcgctg tgctttgggg attttcaggt
    21961 tgatccagcc cgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
    22021 cgccgccgat gtcaattaaa taacggtgaa tacccgcaat cacgccggta atgatgccca
    22081 cccacgggcc aaacagaatc ccgcctgaca tcaccgcgat aatacgtacg ttcaccagcg
    22141 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
    22201 taacggcgag caactctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
    22261 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
    22321 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
    22381 ggtaacctga aggctgatga ccagcaggcc ttttttgagg aaaaaccaca attttaaaag
    22441 gggattgaaa gataaatggt gtattagcaa tgcatctctt g
//
LOCUS       DACADA010000069        22296 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.070, whole genome shotgun sequence.
ACCESSION   DACADA010000069 DACADA010000000
VERSION     DACADA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.070"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            91..306
                     /gene="bdm"
                     /locus_tag="ISB17_002586"
     CDS             91..306
                     /gene="bdm"
                     /locus_tag="ISB17_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAP1980503.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            408..545
                     /gene="sra"
                     /locus_tag="ISB17_002587"
     CDS             408..545
                     /gene="sra"
                     /locus_tag="ISB17_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAP1980504.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            701..2398
                     /locus_tag="ISB17_002588"
     CDS             701..2398
                     /locus_tag="ISB17_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP1980505.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2532..3542
                     /gene="adhP"
                     /locus_tag="ISB17_002589"
     CDS             2532..3542
                     /gene="adhP"
                     /locus_tag="ISB17_002589"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAP1980506.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3728..4005
                     /locus_tag="ISB17_002590"
                     /pseudo
     CDS             3728..4005
                     /locus_tag="ISB17_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Killer protein"
     gene            4005..4289
                     /locus_tag="ISB17_002591"
     CDS             4005..4289
                     /locus_tag="ISB17_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP1980507.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4339..4992)
                     /locus_tag="ISB17_002592"
     CDS             complement(4339..4992)
                     /locus_tag="ISB17_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1980508.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4985..5869)
                     /gene="fdxH"
                     /locus_tag="ISB17_002593"
     CDS             complement(4985..5869)
                     /gene="fdxH"
                     /locus_tag="ISB17_002593"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP1980509.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            complement(5882..8929)
                     /gene="fdnG"
                     /locus_tag="ISB17_002594"
     CDS             complement(5882..8929)
                     /gene="fdnG"
                     /locus_tag="ISB17_002594"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8342..8344),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP1980510.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9161..10042
                     /gene="yddG"
                     /locus_tag="ISB17_002595"
     CDS             9161..10042
                     /gene="yddG"
                     /locus_tag="ISB17_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAP1980511.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10315..11703
                     /locus_tag="ISB17_002596"
     CDS             10315..11703
                     /locus_tag="ISB17_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAP1980512.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            11798..15538
                     /locus_tag="ISB17_002597"
     CDS             11798..15538
                     /locus_tag="ISB17_002597"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP1980513.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            15535..17079
                     /gene="narH"
                     /locus_tag="ISB17_002598"
     CDS             15535..17079
                     /gene="narH"
                     /locus_tag="ISB17_002598"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP1980514.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            17079..17774
                     /gene="narW"
                     /locus_tag="ISB17_002599"
     CDS             17079..17774
                     /gene="narW"
                     /locus_tag="ISB17_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP1980515.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            17771..18451
                     /gene="narI"
                     /locus_tag="ISB17_002600"
     CDS             17771..18451
                     /gene="narI"
                     /locus_tag="ISB17_002600"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP1980516.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            18531..19424
                     /locus_tag="ISB17_002601"
     CDS             18531..19424
                     /locus_tag="ISB17_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAP1980517.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(19520..20365)
                     /gene="nhoA"
                     /locus_tag="ISB17_002602"
     CDS             complement(19520..20365)
                     /gene="nhoA"
                     /locus_tag="ISB17_002602"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HAP1980518.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            20540..21109
                     /locus_tag="ISB17_002603"
     CDS             20540..21109
                     /locus_tag="ISB17_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP1980519.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            complement(21180..22289)
                     /locus_tag="ISB17_002604"
     CDS             complement(21180..22289)
                     /locus_tag="ISB17_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAP1980520.1"
                     /translation="MSIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDFGET
                     HLDFLKQYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITPIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         5296 a         5819 c         5691 g         5490 t
ORIGIN      
        1 cgaagcagca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
       61 accataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca
      121 acagcagaac ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcacggtgtt
      181 gtcactgaag atgacattct catggagctg accaaatggg tggaagcttc tgataacgac
      241 atcctcagtg atatctacca gcaaacgatc aattatgtgg tcagtggtca gcaccctacg
      301 ctttaaggtg ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc
      361 tacggcgatg ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc
      421 gtcaggcacg tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta
      481 ccgaaggtga caaatccagc gtggtaaata atcccaccgg cagaaaacgc cccgctgaaa
      541 agtaattcat aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt
      601 ttttgttttt atctgcttta tacttggtgc cgacgccctg gcggtaatgc aaagacgata
      661 aaagccccca gggatggata ttcaaaaaag agtgagtgac atggaaccaa aaacaaaaaa
      721 acagcgttcg ctttatatcc cttacgctgg ccctgtattg ctggaatttc cgttgttgaa
      781 taaaggcagc gccttcagca tggaagaacg ccgtaacttc aacctgctgg ggttactgcc
      841 ggaagtggtc gaaaccatcg aagaacaagc ggaacgagca tggatccagt atcagggatt
      901 caaaaccgaa atcgacaaac acatctacct gcgtaacatc caggacacta acgaaaccct
      961 cttctaccgt ctggtaaaca atcatcttga tgagatgatg ccagtcattt acaccccaac
     1021 cgtcggcgca gcctgtgagc gtttttctga gatctaccgc cgttcacgcg gtgtgtttat
     1081 ctcttaccag aaccgccaca atatggacga tattctgcaa aacgtaccaa accataatat
     1141 caaagtgatt gtggtgactg acggcgaacg tattctgggg cttggtgacc agggcatcgg
     1201 cgggatgggc attccgatcg gtaaactgtc gctctatacc gcctgtggcg gcatcagtcc
     1261 ggcctatacc cttccggtgg tgctggatgt cggcaccaac aaccaacagc tgcttaacga
     1321 tccgctgtat atgggctggc gtaatccgcg tatcactgac gatgaatact atgaattcgt
     1381 tgatgaattt atccaggctg tgaaacaacg ctggccggac gtactgttgc agtttgaaga
     1441 cttcgcacaa aaaaatgcga tgccgttact taaccgctat cgcaatgaaa tttgttcttt
     1501 taacgatgac attcagggca ccgcggcggt aacagtcggc acactgatcg cagccagccg
     1561 cgcggcaggc ggtcagttaa gcgagaaaaa aatcgtcttc cttggcgcag gttcagcggg
     1621 atgcggcatt gccgaaatga tcatcgccca gacccagcgc gaaggattaa gcgaggaagc
     1681 ggcgcggcag aaagtcttta tggtcgatcg ctttggcctg ctgaccgaca agatgccgaa
     1741 cctgctgcct ttccagacca aactggtgca gaagcgcgaa aatctcagtg actgggatac
     1801 cgacagcgat gtgttgtcac tgctggatgt ggtgcgtaat gtaaaaccag atattctgat
     1861 tggcgtctca ggacagaccg ggctgtttac ggaagagatc atccgtgaga tgcataaaca
     1921 ctgtccgcgt ccgatcgtga tgccgctgtc taacccgact tcacgcgtgg aagccacacc
     1981 gcaggacatt atcgcctgga ctgaaggtaa cgcgctggtc gccactggca gtccgtttaa
     2041 tccagtggta tggaaagata aaatctaccc tatcgcccag tgtaacaacg cctttatttt
     2101 cccgggcatc gggctgggtg ttattgcttc cggcgcgtca cgtatcaccg atgagatgct
     2161 gatgtcggca agtgaaacac ttgctcagta ttcgccgctg gtcctgaacg gcgaaggtct
     2221 ggtactaccg gaactgaaag atattcagaa agtctcccgc gcaattgcct ttgcggttgg
     2281 caaaatggcg cagcagcaag gcgtggcggt gaaaacgtct gccgaagctc tgcaacaggc
     2341 cattgatgat aatttctggc acgccgaata ccgcgactac cgccgtacct ccatctaagc
     2401 ctgcgcccgg tagtgagggc taccgggcaa ttttcctctc cctttttcag atctcatcca
     2461 tactgggtag tggcgaataa atctcatttg cctcacctgc tatgcagaac atcatccgaa
     2521 aaggaggaac tatgaaggct gcagttgtta cgaaggatca tcatgttgac gttacggata
     2581 aaacactgcg ctcactgaaa catggcgaag ccctgctgaa aatggagtgt tgtggtgtat
     2641 gtcataccga tcttcatgtt aagaatggcg attttggtga caaaaccggc gtgattctgg
     2701 gacacgaagg tattggtgtg gtggcagaag ttggccctgg tgttacttca ttaaaaccag
     2761 gcgatcgtgc cagcgtggcg tggttctatg aaggatgcgg tcattgcgaa tactgtaaca
     2821 gtggtaacga aacgctctgc cgttcagtta aaaatgccgg atacagcgtt gatggcggga
     2881 tggcggaaga gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact
     2941 cggcggcggc cagcagcatt acctgtgcgg gggtcactac ctacaaagcc gttaagctgt
     3001 caaaaattcg tccaggacag tggattgcta tctacggtct tggcggtctg ggtaacctcg
     3061 ccctgcaata cgcgaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg
     3121 aacagttaaa actggcaacc aaaatgggtg cagatttagc gattaactca cgcaccgaag
     3181 acgccgccaa aattgtgcag gagaaagccg gtggcgctca cgcagcggtg gtgacagcgg
     3241 tagctaaagc tgcgtttaac tcggcggtgg atgctgtccg tgcaggtggt cgtgtcgtgg
     3301 ctgtcggtct gccgccggag tctatgagcc tggacatccc acggctggtg ctggatggta
     3361 ttgaagtggt cggttcgctg gtcggcacgc gtcaggatct aaccgaagcc ttccagtttg
     3421 ccgccgaagg caaagtggtg ccgaaagtcg ccctgcgtcc gttagaggac atcaacacca
     3481 tctttaccga gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcgct
     3541 aagaggtctt tgctgcgact gccatgttcg ggtcgcagcc tgtttcgcga gacgctttcc
     3601 gtaatttcac tcttcttctt ttcctccccc atatttcccc tcacaattcg cgaaaacgta
     3661 gcgaaatttg ttgcgctgca cggaacaata gtgatagtgt tccttttgag ggaacaagga
     3721 tgaagagatg ataatgaatt tcagacacaa gggattgcgt gacttgtttc ttctcggcaa
     3781 aacctccggc gttataccga cgcaagtcaa acgattacgc caccactcgc tgtgattgat
     3841 gcagcatgct gtcttgctga tatcgatatg cccggttacc gactacatcc gttaagcggc
     3901 gatcgcgatg gaatttgggc gatatctgtc tcgggcaact ggcgaatcac atttgaattc
     3961 gtcaatggcg atgcatacat actggattac gaggactatc actgatgaaa atggccaatc
     4021 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     4081 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     4141 cagctttgac gccagaaatg gcaataaaac tttccgtggt gatcggcagt tcgccgcaaa
     4201 tgtggctgaa tctgcaaaat gcctggagtc tggcagaggc ggaaaaaacg gtggatgtgt
     4261 cgcgactgcg ccgtctggtt acgcaataaa aaatggcgct tcttaaagcg ccattttcag
     4321 cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt ctgccttctc
     4381 gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca ccttcccttc
     4441 gatcatccct ttaatcgatc ctttcaccca gaatgccata tacatatgga tcaggatggc
     4501 gtggatcagg atgatacccg cagccgcatg gatcagcagg ctataacgga caacctgtat
     4561 cgggaagtac tgcgcaaagt acggacgcca gataatcact ccggtcacca gcagcacgaa
     4621 aatcatgctc atgattgacc agaacatcat cttttgcccg gcgttgtact taccgacatc
     4681 cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc acggaatatc
     4741 tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca gcgcgacgaa
     4801 aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg
     4861 cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa
     4921 gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca caatcatttt
     4981 cgacttactc atgatttccc tcctcctcat cgtccacttc cttattcggg ccaataccga
     5041 tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc agcggtttca
     5101 acgcgccttt ccacaggctt accgaggtat cgatcttcgg atctttcggc agaccatgat
     5161 acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca ccgacccctt
     5221 ccgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt
     5281 ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc ggacaggttt
     5341 tcacacaagc cggttcctgg ccgacgctga cgcgatcgac acagagcgtg catttatata
     5401 cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca atgcagtaac
     5461 cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac tgaatgattg
     5521 caccagcaga cgggcatgcc ttcaggcagc ccggatcttc acagtgcata cagccgtctt
     5581 tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc
     5641 aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc cccacttcat
     5701 cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg atacaagtgg
     5761 aaacgtcgat aagtttcgcg acttctgctt tgtaatcacg cacctgagaa ggcggcgtga
     5821 tggagttagt tgcggacctt ttgataatgt cctgcgtttc catagccatc tgttcgcccc
     5881 cttacgcctt ctcgatgttt actaagaacg ctttatattc cggcgtttgc gagtttgcat
     5941 caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca ccttcaaagc
     6001 cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc agcggtttca
     6061 gacggcgcgt taccacagcc accgcgcgga taaagccacg cttgctggag acagtgacac
     6121 gatcgccatt ggcaatgcct ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt
     6181 ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag tgctccgtca
     6241 gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc agcgcgtctt
     6301 gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcaacggg ttagtgccca
     6361 gcggcgtttc aattggctcg tagtgttccg ggaacggacc ttccgccatt ttgttgatgg
     6421 caaacaggcg tcccatccct tccggttgca tgataaacgg cccggttggc gtacccggcg
     6481 cggcattgcc gaagtccgga atatcgttac ccgtccactt gctaccgttc cactggatca
     6541 gcatccgttt cggatcccac ggtttaccgt tgatatccgc cgaagcacgg ttgtagagta
     6601 cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga ccggacgggt
     6661 cggagttatc gcggttagcc atctggttgc cctgctcggt ccagctaccg gtgtagatcc
     6721 agcaagaaga tgcggttgta ccgtcatcac gcagatgtgc aaagctactc agcaactgac
     6781 ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca tagccgttat
     6841 tctctttcgc cacttcatca gactgcggtt cgtgcggctg cttgtagttc cagctcatct
     6901 tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc agatgatggt
     6961 agatacccgc cagaatttcg ccgtcgttac gtgcttcgcc cggcgcgtcc tgacctttcc
     7021 agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttca gcaaagcagg
     7081 tcgaaggcag acggaatact tcagtctgaa tagatgccgg atcgacatcg ttcgactcgc
     7141 cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc accatgtact
     7201 tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca accgggttaa
     7261 agccctggca gaaataaccg gtgactttac cttcatccat catgttgaaa tacttgatga
     7321 cgtcgtaggt ctggtcccac ttcggcagcc agtcgtagcc ccagttgttc tctttctgcg
     7381 cggcatcgcc gtagaaagat ttcatcaggc taacgaaaaa cttcggatag ttgctccagt
     7441 agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat
     7501 caacctgttt ttctgacggc agcgtcagat aacctggcag gctggtagag agcaggccta
     7561 agtcagtcag gccctgaatg ttggagtgac cacgcaatgc gttcacgccg ccaccggcca
     7621 tacccatgtt accgagcagc aactggatca tcgccatcgt acggatgttc tgcgcaccca
     7681 cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg
     7741 tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg caaatgtttt
     7801 ctactacgtc cggcgtgtag cgggaaacgt gcgctttcag cagattccat acacagcgcg
     7861 gatgagtcag cgtgtcatcg cgtttcgcat agccgttttc atcgaactga tagttccagg
     7921 acgacttatc gtattgacgt ttttcagcgt cgtagccgct gaacagaccg tcttcgaaag
     7981 caaaatcatc acgcaccagc aggctggcgt tggtgtaatg cttaacgtat tcggcgttga
     8041 ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg
     8101 aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg ggatcgacaa
     8161 cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat
     8221 gcgcttcagc agcgttaccg cccatcacca ttacgacgtt agcgtttttg atatccaccc
     8281 agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt
     8341 gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca aatttttgag
     8401 tcagcatccc ggtttcgttg ctggcaccgg aagcacacag cataccggta gaaagccaac
     8461 ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg tcggctttca
     8521 tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat ttgtcagaac
     8581 ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacataa tccagcaaac
     8641 cggccccttt cgggcacagc gcaccacgac ttaccggatg atccgggtca ccttcaatgt
     8701 gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc aatagcccgc
     8761 aacctacgga acagtatgtg caggtgttac ggatctcttt cgcgcgtaat aatttgtagt
     8821 ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgctgctact gttgttcccg
     8881 ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct
     8941 tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg
     9001 aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc
     9061 aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt
     9121 cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgtc aaaaagcaac
     9181 gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt
     9241 cagtgaggga ctcggcccgg tcggcggcgc agctgctatc tattcattaa gcgggctgct
     9301 gttaatcttc acggttggat ttccgcgtat tcggcagatc ccgaaaggct atttactcgc
     9361 cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag ggtatgcggc
     9421 gacccgtcat caggcgattg aagtggggat ggtgaactat ctatggccca gcctgacaat
     9481 tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac caggattatt
     9541 attagccctg gtcggcgtct gctgggtgtt aggcggtgac aatgggttac attatgatga
     9601 aattatcaat aatatcacca ccagcccatt gagttatttc ctggcgttca ttggggcgtt
     9661 tatctgggca gtctactgca cagtaacgaa taaatacgca cgcggattta acggaattac
     9721 cgtttttgtc ctgctaacgg gagcaagtct gtgggtttac tattttctta cgccacaacc
     9781 agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat ttaccttagg
     9841 atttgcttat gctgcatgga atgtcggtat attacatggc aacgtcacca taatggcggt
     9901 aggttcgtat tttacgcccg tcctttcctc agcgcttgca gccgtgctgc tcagcgcccc
     9961 gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc tgctctgctg
    10021 gctggcgaca cgtcgtggct aaaataattg tcgggtctac ggcccggcag ttaatcagat
    10081 aaattctgac aatattaaaa aataaatttc aaataccaga aagtcatttt agttacattt
    10141 agtaacacat atctgtctgt ttaatccaca ttctgccccc gcagttacgc tttcttatct
    10201 attcttctaa tcagcacttc acctgattca tccttaattc taataactgg ctattgcgcc
    10261 ttacctcctg ttttgtcttt ttttcattac caatgtgtgc atgtgaggaa caatatggca
    10321 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
    10381 ttctgggaaa ataaaggaaa gcatattgca cgaagaaacc tctggatatc agtcagttgt
    10441 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttactgttaa tctcaataaa
    10501 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
    10561 ggcgcattat tgcgtgtccc ctactccttt atggtgccta tattcggtgg acgccgatgg
    10621 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcag
    10681 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
    10741 gcgaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
    10801 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
    10861 gcaccgctgg tcatttttgt ccccgtattt gcctttctcg gcgtcaatgg cgtaccgcag
    10921 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtgcc attactggcg
    10981 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
    11041 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
    11101 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
    11161 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
    11221 cgatcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac
    11281 ttcattttta tggcgatttt cagcgccctg ctgttcctta ccttaccggg cacaggctcc
    11341 ggtaatttca tcgcatttta cgccgtattt atggggctgt ttctgaccgc gggtctggga
    11401 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataacaat ttatcgggtg
    11461 aagatgaaag gcggtagtga tgagcaagct caaagagaag ccgtcaccga aacggcagcc
    11521 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
    11581 ggcatgtcgc tcaatatgac cggctctccg gtgggcgcga tgaaagtgtt tttaatcttc
    11641 tacatcgttt gtgtgctact gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
    11701 taagagatcg atgcctgaca cgtcttatca ggctacaacc gtgtcgcatc cgacaaggca
    11761 tcagatccca attcattcct ggagcaggag ttatgtcatg agtaaacttt tggatcgctt
    11821 tcgctacttc aaacaaaagg gcgaaatctt tgccgatggt cacggacagg tgatgcatag
    11881 caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc
    11941 cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta aaaatggtct
    12001 ggtgacctgg gaaatccaac agaccgatta cccgcgcact cgccctgacc tgccgaatca
    12061 cgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctctaca gcgcgaaccg
    12121 cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag ccctcaagca
    12181 acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa agtgcctgag
    12241 ctacaaacaa gtgcgtggac gcggtggttt tatccgctcc aactggcagg agctaaatca
    12301 gctgattgcc gccgctaacg tctggaccat caaaacttac ggcccggatc gcgttgccgg
    12361 tttctcgccg attccggcga tgtcgatggt ttcttacgct gccggaacac gctatctgtc
    12421 gctgttaggt ggcacctgtt taagcttcta cgactggtat tgcgacctgc cgcccgcctc
    12481 gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt ataactccag
    12541 ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg cccatttctt
    12601 taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact actctgaagt
    12661 ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagtg ccctggcaat
    12721 ggcaatgggc catgtcattt taaaagagtt tcatctcgat aatcccagcg attattttat
    12781 caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggaac ctcgcgacga
    12841 cggcagctac gttcccgggc gcatggtccg cgcatctgac ctggtggatg gactgggcga
    12901 aagcaataat ccgcagtgga aaaccgtggc agttaatacc gcaggtgaat tggtagtgcc
    12961 gaacggttcg attggttttc gttggggaga aaaaggcaaa tggaacctgg aatccattgc
    13021 cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg ccgttgcagg
    13081 cgtggccttc ccctactttg gaggcattga aaatccgcat tttcgcagcg taaaacacaa
    13141 tcctgtcctg gtgcgccaat tgcccgttaa aaatctgaca ttagccgatg gtagcacctg
    13201 tccggtggtc agcgtttatg atctggtact ggcaaattac ggcctcgatc gcgggctgga
    13261 agatgaaaac agcgcgaaag attacgctga aatcaaacct tacacccctg cctggggcga
    13321 gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgtgaat ttgccgatac
    13381 cgctcataaa acgcatgggc gctcgatgat tattctcggc gcaggtgtta accactggta
    13441 tcacatggac atgaactacc gcgggatgat caatatgctg atcttctgcg gttgtgtcgg
    13501 gcaaagcggc ggcggctggg cgcactatgt cggtcaggaa aaactgcgcc cacaaaccgg
    13561 ctggttgccg ctggcctttg cgttcgactg gaaccgtcca ccgcgccaga tgaacagcac
    13621 ctcgtttttc tacaatcact ccagccagtg gcgctatgaa aaagtcaccg cgcaggaatt
    13681 gctctcaccg ctcgccgatg ccagtaaata cagcggtcac ctgattgatt tcaacgttcg
    13741 cgccgaacgt atgggctggc tcccttctgc gccgcagctg gggcgtaacc cgctctccct
    13801 gaaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc aggcgctgaa
    13861 atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc atccgcgtaa
    13921 tttgtttgtc tggcgttcta acctgcttgg ctcctccggc aaaggccacg agtatatgca
    13981 gaagtatctg ctggggaccg aaagcgggat tcagggagag gaactcggtg ccagcgacgg
    14041 gatcaaacca gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct
    14101 ggtgacgctc gatttccgca tgtccagtac ctgcctgttc tccgatatcg ttctgcccac
    14161 cgccacctgg tacgaaaaag acgatatgaa tacctcggat atgcatccgt ttattcatcc
    14221 gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa tctacaaagg
    14281 tatcgctaaa gccttttcgc aagtgtgcgt ggggcatctc ggcaaagaaa ccgacgtggt
    14341 attacaacca ctgctgcatg actccccggc agagctttca cagccgtgtg aagtgctcga
    14401 ctggcgcaaa ggcgagtgcg atctgattcc aggcaaaacc gcgccgaata ttgtggcggt
    14461 ggagcgcgac taccctgcta cctatgaacg ctttacctcg ctcgggccat tgatggacaa
    14521 acttggcaac ggaggtaaag ggatttcgtg gaatacccag gatgaaattg atttcctcgg
    14581 taaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga ttgataccgc
    14641 gattgatgcc tcagaagtga ttctggcgct ggcaccggaa accaacggcc atgttgcagt
    14701 caaagcgtgg caagcgctgg gcgagatcac cggacgcgaa catacccatc tggcgctgca
    14761 caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta aaattatctc
    14821 cagccccaca tggtctggtc tggaaagcga tcacgtctcc tataatgcgg gatacaccaa
    14881 cgttcatgag ctaattccgt ggcgcacact gtcgggccgt caacagcttt atcaggatca
    14941 tccgtggatg cgtgcctttg gtgaaagcct ggtggcttat cgcccgccta tcgacacccg
    15001 cagtgtcagt gaaatgcgcc aaatcccgcc aaacggcttc ccggaaaaag cccttaactt
    15061 cctgacaccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
    15121 gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc gtgaactgac
    15181 cattgtcgat aacgactggg tggaagtgtt taacgccaat ggcgcgctga cggcccgcgc
    15241 ggtggtcagc cagcgtgtac cgccgggcat gaccatgatg tatcacgctc aggaacgcat
    15301 tatgaatatt cctggttcgg aagtaaccgg catgcgcggc ggcattcata actcggtcac
    15361 ccgcgtttgt ccgaaaccaa cacatatgat tggcggttac gcgcagctgg cctggggctt
    15421 taactactac ggcactgtcg gctctaaccg cgacgaattc atcatgatcc gcaagatgaa
    15481 gaacgttaac tggctggatg atgaaggtcg cgatcaggtt caggaggcga aaaaatgaaa
    15541 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
    15601 tcggtgacct gtaaaaacgt ctggaccggt cgcgaaggca tggagtacgc atggtttaac
    15661 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggatgagtgg
    15721 caaggtggct gggtgcgtga tgtgaatggc aagatacgcc cgcgtctggg cagcaagatg
    15781 ggcgtaataa ccaaaatttt cgccaacccg gtagtgccgc agattgacga ttactacgaa
    15841 cctttcacct tcgactacga acatttgcat agcgcgccgg aaggcaaaca tattcccacc
    15901 gcccgtccgc gttcactgat tgacggcaaa cggatggaca aagtgatctg gggcccaaac
    15961 tgggaagaac tgctgggcgg cgaatttgaa aaacgagccc gcgaccgcaa cttcgaggcc
    16021 atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct gccgcgcctg
    16081 tgcgaacact gcctcaatcc cagctgcgtg gcgacctgcc ccagcggcgc tatctacaaa
    16141 cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg gcgtttgtgc
    16201 attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa
    16261 tgtatcttct gttatccgcg aattgagtcc ggtcagccaa cagtctgctc cgaaacctgc
    16321 gtgggtcgca tccggtatct gggcgtgctg ctctacgacg ccgaccgcat cgaagaagca
    16381 gcgagtaccg aacgagaggt cgatctctat gaacgccagt gcgaagtgtt cctcgatccg
    16441 cacgatccct cagtgatcga agaagccctg aaacaaggta ttccacaaaa cgtgattgac
    16501 gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc gctaccgctg
    16561 caccctgaat accgcaccct gccgatggtc tggtacgttc ctccgctgtc accgattcag
    16621 tcctacgctg atgcgggagg tttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc
    16681 ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggtgatac cggtccggta
    16741 ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca aaccgtggaa
    16801 ggcgttactg atactcgtgc catcgacgaa gtaggtctga gcgtcgccca ggtcgaagag
    16861 atgtatcgtt acctcgccat tgccaactat gaagatcgtt tcgtcatccc gacaagccat
    16921 cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac ctttggcgac
    16981 ggttgccacg gttcggacag caaattcaac ctgttcaaca gtagccgtat cgatgccatc
    17041 aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc aaagtgctcg
    17101 gcctgttgat ggagtaccca gacgagttgt tgtgggagtg taaggacgac gcgctggcgc
    17161 ttatccgccg cgacgcgccg atgctcaccg attttgcact cgacctgctc aacgccccgc
    17221 tgctggataa acaggccgaa tggtgtgaag tgtttgaccg cgggcgcacc acgtcgctgc
    17281 tgctgtttga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg gtggacctgc
    17341 tggcggagta tgaaaaggtc ggtctgcaac tgaattgtag ggaactgccc gattatctac
    17401 cgctgtatct ggagtattta agcgtacttc cggacgatca ggcgaaagaa ggattactca
    17461 acgttgcgcc gatcctcacc ctgcttggcg gacgcttaaa acaacgcgag gcaccgtggt
    17521 acgcgttatt tgacgccctg ttgcaactgg caggaagccc tctgtcaagt gacagtgtca
    17581 caaaacagat tcgcagtgaa gagcgtgacg acacccgcca ggcgctggat gcggtgtggg
    17641 aagaagaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc tcgccgctta
    17701 atcaatatca gcgacgcttt agccaggatg tcgcgccgca gtatgtcgac atcagtgcgg
    17761 gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc cgtacttgtg
    17821 tggcacggtt tttatccttg gaagctggct gcgctacgac tacgggcagt acacctggcg
    17881 cgccgcctcc agccagatgc tggataaacg cgggatggtg ctgtggtcaa acttgttcca
    17941 tatcggcatt ttggggattt tcttcgggca cctgttcggc atgttaacgc cacactggat
    18001 gtacgcgtgg tttctgccgg tggcggtaaa acagcagatg gcgatgatcc tcggcggaat
    18061 atgcggggta ttgacgctga ttggcggtgc ggggctactg gtgcgtcgtt taacaaatcc
    18121 gcgtatcagg gcgacgtctt ctggtgcgga tatcctgatc ctcgccattt tgttaattca
    18181 gtgcattctc gggttaacca ccattccttt ttcagcacaa catccggacg gcagtgaaat
    18241 gatgaagctg gtgggctggg cgcaaagcat tgtcactttc cagggcggtg catctacgca
    18301 tcttgatggt gtcgccttta tattccgcgt tcatctggtg cttggaatga ccatcttcct
    18361 gctcttcccg ttcacccgac tggtgcatgt ctggagcgcg ccgtttgagt actttactcg
    18421 tcggtatcag attgtgcgtt cgcggcgtta attctcacgc gggccggata gcccttcgct
    18481 ctccggccac ttgcaggcta tgatagttct ccagccacag gagaacgtta atgaaaccgc
    18541 aagtgtacca cgtcgatgct tttacctctc aaccgtttcg cggcaattct gccggtgttg
    18601 ttttccccgc cgataatctc agtgaagcgc aaatgcagct tatcgcccgc gagttaggtc
    18661 attcggaaac tgcttttctg ctgcacagcg acgacagcga tgtgcgcatc cgctacttta
    18721 cgccaacggt tgaagtgcca atttgcggtc acgcgacggt ggctgcgcac tatgtacgtt
    18781 caaaggtatt aggtttggga aattgcacgg tctggcaaac gtcgctggca ggaaaacatc
    18841 gtgtgactat cgaaaagcaa aatgatgatt atcgtatttc gctggaacaa ggtacgccag
    18901 gctttgagcc accactgaca ggtgaaacac gtgcagcgat tatcaacgcg cttcatctca
    18961 ctgaggacga tattctgcca ggcttgccaa ttcaggtggc aacaacagga cactcaaaag
    19021 tgatgatccc gttgaaacca gaagtggata tcgacgccct ttcgcctgat cttgctgcgt
    19081 tgaccgctat cagcaaacag attggctgca atggtttctt cccgttccag atccgcccag
    19141 gcaaaagcga aaccgacggt cgcatgttct cgcctgcgat tggtattgtg gaagatccgg
    19201 tgaccgggaa tgccaatggt ccgatgggcg catggttagt acatcacaac gtattgcccc
    19261 acgatggcaa cgtgttgcgt gttaaaggcc atcaggggcg cgcattgggg cgtgatggtg
    19321 taattgatgt gacggtgaca attcgcgata accaaccgga gaaagttacc atttccggca
    19381 cggcggtgat tctattccat gctgaatggg ccattgattt ttgatgccgt tgcctgatgc
    19441 gcttcgctta tcaggcctac aaccggacta atgtaggccg gataaggcat ttacgccaca
    19501 tccggcaacc tgacataaat tattttcccg cctccgggtg cgtatcaaac gccgccatca
    19561 ccagcgctaa ctcatccaca gtaaagccat gtttcgcatc atctacgccc agaccaaact
    19621 gttcctgcat cacggcatat aacgacgcca catctggtag atttcgctgc tcaactgcgt
    19681 gcccattttc ataatgggta aaatgaaaat tggtcagcgt tagcttgcca ccgtccggca
    19741 aatggcgaca catcagcaaa tgatggcgaa aatgcgactg cggccagtgc gccgaccaga
    19801 aattgcctat cacataatcg ctttgttgct gctcgcagag atcaaaacgg tacatcgact
    19861 gccagtgctg atgatgatta aactgcaata cccaatcatc accctcttgc aacaaccgat
    19921 actctccgtg tggcgtggtt tgcatgatat ccggcattaa acgaatcggc gcggttagcg
    19981 tctgcccacc gaaaccgaca tcagcaatcc atttttcccc ttccagttcc accaacagca
    20041 aacgatgggt gcgcggcggt aatgctggcg gatttgataa cactacgcga cctaacaagc
    20101 tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac
    20161 agtaaccgcc acgacgggct atcaccagtt tctcttccag cgattgatca tcaagctgta
    20221 tttccctcgg cagcaaaacg tcgagatttt caaacggaat ggtgcaattg tgtttcaggt
    20281 gcaatgcacg cagcgtatca atattggccg cagcagctcc caaccagtta atacgggcaa
    20341 aataatgatt cagaatgggc gtcatcaggc gtttccctta gtggttttct caacttataa
    20401 cctggaatag acgccgcact ggctattttg tgcgatcgcg gacatgcatc acgttcgcaa
    20461 atcgccaatc ccgttaattt cgttacttat tgcgttacga gctacgcttt taaaacaccc
    20521 ttcatagcga ggcgatatcg tgagccaatt cattcccgtc gaattacacc atgccagctg
    20581 tctgttaaat catggcccaa ctgtcatgat caccagtttc gatgagcaat cccagcggcg
    20641 taacattatg gcagcagcct ggtcgatgcc cgtagagttt gaaccgccac gcgtggcgat
    20701 tgtggtagat aaatcgacat ggaccaggga gttgattgaa ggtaacggta aatttggcat
    20761 cgttatcccg ggcgttgcgg cgactaactg gacgtgggcg gtgggaagtg tgtcgggtcg
    20821 tgaagaagat aagtttaatt gctacggcat tccggtagtg agaggcccgg tgcttggttt
    20881 gccgctggtc gaggaaaaat gtctggcgtg gatggagtgt cgcctgctac ctgcgacttc
    20941 cgcgcaagaa aaatacgata cgctatttgg cgaagtggta tctgcagcgg cagacgcacg
    21001 agtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt
    21061 aggtgctggc atgtttgtta ccagtggtaa gcgagttacg gcaggttaag cattacatgc
    21121 tgtaaccggg tttctttatc aaagcctcca tttggggcgc gatttcggcc agggcaagat
    21181 tacgaaagcc cgctccccgc aaggactgac gcgagatagt ttctgtccat ggctgctttt
    21241 cgcatcttac gtcttaaccc tgccttgaat accttatcat tcgttaaaat attaatagcg
    21301 atgtgccgta tccctgaaaa taattctgcg gcgtttcctc ttcttatttt gcagtcgtct
    21361 tcattcatta ccacgtccag acgccagtgc agcttattct ccacgtgcca gtggtttcgg
    21421 attgctgtgg cgaacttctc tgcggttaaa tcagcagaac tgatataata tctgaccgtc
    21481 atttctggct ctttcttttg ttctgctatt atggaccgaa aggagactgc cacgcataat
    21541 ttcttcagtc ctttccattc gaacgtgaaa tcaataagtt catcagggac atcgcaaaca
    21601 atatgaagac ggatttcttc tctgccgtga ctcttttcac taatggcgta actgtcatgt
    21661 tctggattat ttaattcttt cagtggaaat ttttcctcaa aggctttatt aagccgcccc
    21721 tgatttcctt ttacagcgaa taaataatca ccgccctgtt tttgtatctt ctctgcaata
    21781 tctttctggc aacccatcgc atcagttgtg atgatttttc ctttaatatc cagcatgtta
    21841 agaagttcag ggataggtgt aatttcattg gatttctcat ccgtcctgat ctgtccgatg
    21901 accagactgt gcattgttga gaacgcacta atgacatgaa tcgctccctt gcggcgactc
    21961 ttgtcataag aatgccggag tgtttttcca tcaattgcga tgacgtcttt atcatctgaa
    22021 gaatggcagt cacgcatcca gttaataaag cactcgtgaa attttgcagg actgatacag
    22081 gatacaactc tggcaatggt atcgtgaaca ggaataccat tatcaaaatc accatattgc
    22141 ttcaaaaaat cgagatgtgt ttccccaaaa tcctctatat cttcccagcc ttctgcaccc
    22201 gaaataacgg cacaaatagt caacagtaga atatccgata atttatgttc cactttccag
    22261 gcttgtctgt aatcggggat aatagaaata tgttcc
//
LOCUS       DACADA010000070        22052 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.071, whole genome shotgun sequence.
ACCESSION   DACADA010000070 DACADA010000000
VERSION     DACADA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.071"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..356)
                     /locus_tag="ISB17_002605"
     CDS             complement(<1..356)
                     /locus_tag="ISB17_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP1980521.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLD"
     gene            complement(614..1867)
                     /locus_tag="ISB17_002606"
     CDS             complement(614..1867)
                     /locus_tag="ISB17_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAP1980522.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2099..3430
                     /gene="argA"
                     /locus_tag="ISB17_002607"
     CDS             2099..3430
                     /gene="argA"
                     /locus_tag="ISB17_002607"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAP1980523.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQTRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3512..5338)
                     /gene="recD"
                     /locus_tag="ISB17_002608"
     CDS             complement(3512..5338)
                     /gene="recD"
                     /locus_tag="ISB17_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAP1980524.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEVLAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5338..8880)
                     /gene="recB"
                     /locus_tag="ISB17_002609"
     CDS             complement(5338..8880)
                     /gene="recB"
                     /locus_tag="ISB17_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAP1980525.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8873..11761)
                     /gene="ptrA"
                     /locus_tag="ISB17_002610"
     CDS             complement(8873..11761)
                     /gene="ptrA"
                     /locus_tag="ISB17_002610"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAP1980526.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11937..15305)
                     /gene="recC"
                     /locus_tag="ISB17_002611"
     CDS             complement(11937..15305)
                     /gene="recC"
                     /locus_tag="ISB17_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAP1980527.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15318..15641)
                     /locus_tag="ISB17_002612"
     CDS             complement(15318..15641)
                     /locus_tag="ISB17_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP1980528.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15626..16033)
                     /locus_tag="ISB17_002613"
     CDS             complement(15626..16033)
                     /locus_tag="ISB17_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAP1980529.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16030..16593)
                     /locus_tag="ISB17_002614"
     CDS             complement(16030..16593)
                     /locus_tag="ISB17_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP1980530.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16584..17054)
                     /locus_tag="ISB17_002615"
     CDS             complement(16584..17054)
                     /locus_tag="ISB17_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP1980531.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17239..18033)
                     /gene="thyA"
                     /locus_tag="ISB17_002616"
     CDS             complement(17239..18033)
                     /gene="thyA"
                     /locus_tag="ISB17_002616"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP1980532.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18040..18915)
                     /gene="lgt"
                     /locus_tag="ISB17_002617"
     CDS             complement(18040..18915)
                     /gene="lgt"
                     /locus_tag="ISB17_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP1980533.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19066..21312)
                     /gene="ptsP"
                     /locus_tag="ISB17_002618"
     CDS             complement(19066..21312)
                     /gene="ptsP"
                     /locus_tag="ISB17_002618"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1980534.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21325..21855)
                     /gene="rppH"
                     /locus_tag="ISB17_002619"
     CDS             complement(21325..21855)
                     /gene="rppH"
                     /locus_tag="ISB17_002619"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAP1980535.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         5338 a         6093 c         5426 g         5195 t
ORIGIN      
        1 tcatcaagga aaacattatt aatttgtata cctgaagcga ctcgggatat tgcattcgaa
       61 atgtgatccg caagaatcaa tagtagcgat ggattgagat ttttttcgag atttttctcc
      121 gcatattgca ctattttttc ggcaacaaac acatactcaa tatctacatg ttcaatcaat
      181 ttatagagtt tgtttttttg ttcatttctg acataaaaaa tccggttagc cggatgctca
      241 gggaccgaca ttccatattt tttgttgtaa ccgacgccag gcccagaaat gataacctcc
      301 tgtccattca tcgatgcctg tacacaatta ttgttcatga ctttttcgat gatcattcca
      361 tggctccaaa aaaaaaggca aggcacgtca gaagtaataa ctacttcaaa cattgccttg
      421 cctgatttaa cagttacaag atgattaact aatgcgtata cattacacgg aattgtgctt
      481 aattaaagag atggtcggct tatttttcaa gccgacattc aaatatccga catcagtcac
      541 aaaaccagcg tctaatattc aggtaaatgt tatctattcg acgaaatata aattcattga
      601 ataaataaaa acattatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa
      661 ttcccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      721 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      781 ttagtacggc aaacccagcc tgttcaactt gatttttatg cagcttgttg attttaccga
      841 gcttattcag caccgcttta ccaaacttaa ggctgtcggc aatggtcagc gactgcacca
      901 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gcttacgcca ccaatcaagt
      961 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg
     1021 agagcgcaaa caccgaggaa ccgctcggct ggcgactggt aaaggcgtcg gcatggatag
     1081 aaacaaacaa gtcagcgcgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
     1141 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc
     1201 gcagacggcg agctatttgc aataccacgt ctttttcgcg tgttttgtat ttccccaccg
     1261 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcccgcc
     1321 ctgctttacc cggttgtgga ccactttgtg ctggcggcac ctgcttttcg aggtcgcctt
     1381 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
     1441 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
     1501 gttttacgtt ttgctttaat tcaaaaacca tacgtacggt ttgcgggtca aattgcccga
     1561 cgcgcgccga cttgatgaac gggtcgtcag cgcggatttg cgcagccatc cccttgagca
     1621 ctgagttcag gtttacatct tcgatatcca ccaccacgcg ttcaggatta ctcaacgcga
     1681 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1741 gccagacgcg caccgccacg acctggctga ccgcagccag gctgacctga cttacgctca
     1801 atagccacat ggcacccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1861 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgaccaattt
     1921 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta taaaacggta aacattcttt
     1981 ttatattcac ggtattactg ataaaaaact cgctcccgca taaaatttac acttgcaccc
     2041 tgcgaaaaaa cagaataaaa atacaataat ttagaataat catgcaaaga ggtgtaccgt
     2101 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     2161 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     2221 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     2281 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcacatcacc acgaaccgct
     2341 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     2401 gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata acacaccgct
     2461 gcagggcgcg catatcaacg tcgtcagtgg taattttatt attgcccagc cgctgggcgt
     2521 cgatgacggc gtggattact gtcatagcgg gcgtatccgg cggattgatg aagacgcgat
     2581 ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcggtcac
     2641 tggcgagagc tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa
     2701 agctgaaaaa atgattggtt tttgctcttc ccagggcgtc actaatgacg acggtgatat
     2761 tgtctccgaa cttttcccta acgaagcgca aacgcgggta gaagcccagg aagagaaagg
     2821 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2881 cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctattgc aagagttgtt
     2941 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     3001 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc aacaaggtat
     3061 tctggtacgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     3121 gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
     3181 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     3241 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     3301 cacgcgcagt attcactggt tccaggaacg tggatttacc ccagtggata ttgatttact
     3361 acccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     3421 tttgggttaa gcgatgcaaa tcgccggatg cgacatgcac aacattcata ggtatgataa
     3481 gacccacatg cgtcgcatca ggcaattacg tttatccccg tgaactaaac aacgccgcca
     3541 gaccactgcg ccgctcggtg cgcgtggcaa ttgccgcact taaaatgcgc tcatcggcat
     3601 acagcgacag acggcgacgc gcgcgggtca ccgcggtgta aaccagctct cgcgttacta
     3661 ccggcgtgcg ttgactcggt aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt
     3721 tatgtaccgt catcgcccac gtcgtttcgt gctctggcag gcgactcggt tgcacagact
     3781 taatattgcc gtccggcatc gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa
     3841 taccaatatc gccattaaac aacccaagcg cgctgtcatt acgggcaatc atcactggtc
     3901 gaccttcgta ccaacgagag tgcggatgac gatgaatttt gcgcttctgt tgcataaatt
     3961 gctcaattcg ctcattcaat ccagccacgc caaacggtcc ttcccgcagg gcgcacaaaa
     4021 gctggtactc attgaacgcc tgaatgatta aatccggctc ggcacgcgct tgcagcagat
     4081 ccagataacg tccgtaaccc gcaagaactt cctcaagcat cgcaatataa tcttcgccac
     4141 tctgtaaaag ccgtttttcg atatcagtaa aatcctgctg aaaaacggtt ttcactgccg
     4201 ttttatcacc tcggttaatc gccgcagcta actgaccaat gccagaatcg ctgccgaaac
     4261 gatagctttt ttgcagcagg cagaggctgt cgcgcaaaga tgccgcttct gtgccagttc
     4321 ctgctggaac gtggcttccc gtcaggcggc tcaactgccc ggcacgctcg gcggtaaagc
     4381 ccgcgttggc ataagcgcag atatcgccca gcacagcccc agcctcaacc gaggccagtt
     4441 gatcacgatc gccgagaaag atcactcgcg catgatcggg caaggcgtcg atcagtctcg
     4501 acatcatagg aagatcgatc attgacgctt catctaccac cagcacatca agatgcagcg
     4561 ggttaccggc atgatgacgt aaacgctggc tacccggctg cgcgcccagc aatcggtgca
     4621 aagtgctggc atcttccgga atgcgtttct tttgttcagc ggtcagcggt aactgtcgca
     4681 aagccttgcc gagagattcg gttaagcgcg cggcagcttt acccgttggt gcagccagac
     4741 ggatacggca gcgttctccg tcggccattt gaattaaggc tgccagcaac ttcgctacgg
     4801 tggtcgtttt accggtgcca ggaccgccgg aaatcaccga aatccgccgc gtcagcgcca
     4861 ctgccgccgc aactttttgc cagttaattt catcgcttac tggaaaaagt ttgtccaggg
     4921 tttgcgccag tagagcttca tcaacctcaa tggcatgatt cacttcgttg aaaaaacgtg
     4981 ccactgtgcg ctcgttacac cacatgcgat tcaaataaag acgatcgcca cagaggatca
     5041 tcggcgttgg ttcatctccc cggctgaccg cttgtgaagc cagcaagcat tcttcccaat
     5101 tttgtagctc accgatttca ctgacacagg tcgccaacag cggatgcgac gcctcgttat
     5161 tttccagtcg tgaaagcggc aaacaaacgt gtccctctcc ggcatcatga cttaatagtg
     5221 ccgccgcgag ggtgacggca ggatgttcat ctcccgccac ggtcagggca aactgcacgt
     5281 ccagcgggcg tagctgtttg tgctccacag cttccagtaa ttgcttttgc aatttcatta
     5341 cgcctcctcc agggtcatac cggcaaacat ctcatccatc aggtcaatca acccggcgtt
     5401 gggtcgggtc gcgtagatcc cttgttgcgg atgttcttta tcaacgccac gcaggaacag
     5461 ataaataacg ccgccaaagt ggcgctcata gtcgtaatca gcaatacgat ggcgcagata
     5521 acggtgcagc gccagggtat aaagctgata ttgcagatca tagcggtgtg cctgcattgc
     5581 cgctgccata gcctgttggg tgtaagccga actgtcttca cccaaccagt tggatttata
     5641 gtcgagcagg taataacgcc cttcgtggcg gaacaccaga tcgataaagc cttttaacat
     5701 gccacgtacc tgcatgaact ccagcggcgg gcagccagca gatagcgggt caaactggcg
     5761 aattagtgca tcaagctggc tggcgatgag cggttcgcta attggcaggt aaaactccat
     5821 ctccacctgt ttatcgcgat cggaaagctg actcaggcta atacccgttt cattgagagg
     5881 tgccaggagg acagccgtga tccactcagt caacaccggt tcccactgcg gttcaaagcc
     5941 accgagctcc agtttttcct gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc
     6001 ttcaaacaaa ctgtgcaaga atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt
     6061 taacgtcggt acttcaacga cgctgaccac gcctgcggca tcaacatcca accgtggcat
     6121 cagatcctga gcgataccgt gaccacgctg ctgcaaaccg gagtagctgg tgacacgcca
     6181 gttatcgccg ggcaatcgtt gtagcgtcct cgcattcagt tctgcagtta atgcatcatt
     6241 aacctgccag ggctggttat caccaatttg tgccgtttgc caggcaatat catcatcgca
     6301 caacgcttca atgcaggtac gaagccctgc cacatcttgc ggttctcctt tttgcagcaa
     6361 acgcccgagc gcactttggt ggacgtcggt atcacctttt ttatcgccac gacggcgcac
     6421 cagcggtgca acgccgagac tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagtaa
     6481 acgcagatct tccgccagac gttcggcctc cgcgaggtcg acgctttctg gcgcagcatt
     6541 aagatccaga acggcctcaa acgagtggcg atcgtgataa aacgcctgat cctggacgcg
     6601 gaaattggta ataaatggca accagaccag cggatattcc agcccttttg atttgtgtat
     6661 cgtgacaatc tgcaccagat gtttatcact ttcgaggcgc atttgttggc tggaggcatt
     6721 actgtctggc tcgaggatat gttgcgataa ccagcgtacc agcgcatgtt cactttccag
     6781 ctgcgttccg gcttcttgta gcagttcgct gatatgcaag atatcggtaa gacgccgctc
     6841 accgcctgcc gttgccagca agttttctgc aatgttacgc gccgacatca gcgcccgcag
     6901 catcggcata acgccacgtt tgcgccagat ttgccgataa ccatcgaact cttcgactac
     6961 cgcatcccac gcatgttcgt cattattcag cgtttcaata tccagcgcat tcagccccat
     7021 cattgacgtt gccagcgcac tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg
     7081 caacagccaa agcatctcct gtgcttccag agtttcaaaa acactgtcgc ggttcgaaag
     7141 gtaaacggaa gggatttcca gcagcgttaa ggcatcgcgc acctgagcgg cctcctggcg
     7201 gctgcgcacc agcacactga tgtccgaagc acgcaccgga cgcgcatcgt cgccgttcat
     7261 cagcaacgct tcgccccgct gtccggcttg tagccagtcg cggatttgcg cagcacatac
     7321 ctgcgccatg gtactttgat agtcgccaac gccgcagctt tcgccttcca tcagccacat
     7381 tttcatcgca ggctgcattt cacctttaaa cacaaagcgt aacgcctgat ttttcccggc
     7441 agatttcaca ggaataaacg gtatttcacg aaacatgaac gcgtcatcag tctggctgaa
     7501 aagtttgtta acgctgttca ccattcccgg tgcggaacgc cagttggtat ctaaggtgta
     7561 gtgagcatga acttcgctac gcgccttcat ataagtgaag atatccgcac cccggaatgc
     7621 atatatggcc tgtttcgggt cgccaatcag caacaatgcg gtttccggct gatggtgcca
     7681 gatacggcga aaaattcggt actgctgggg gtcggtatcc tgaaattcat cgatcattgc
     7741 caccgggaat cgcgtacgga tcgccgctgc caatacctcg ccgctttcac tacgcagcgc
     7801 ggaatcgagc cgacttaaca tgtcatcaaa acccaattcg ccacggcggc gtttttcacg
     7861 cgctactgtt tcgcggatct cagccaatgc gcgggtgatc accagatcgc ggatcgacaa
     7921 tggttctgca agcagttgat cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc
     7981 ggctttcgtg cgatcttcta agaaacgctg agagaatttt tccagcgact ccggcaactg
     8041 atagctattg gtctcttctt ctgcccaggc gctgatcttc tcgatccatt tagcctgatt
     8101 gctacggtta aacttgcgtc gatcaatacc agaagattcg atcagcgcat ccagttcacc
     8161 cactgcgtcg cgccactgct gttttacggc atcaatacgc gccacaattt gcgcgtggcg
     8221 ggaagccagc gtttcatcat cggggggcgg tgctttgata actggcgctt cgccttgcag
     8281 ataacgatta atatcgcgca gcaacgcctg cggccctttc caggtttcaa agacgacctg
     8341 ggcaatttca cgcggcagcg ggtagcagtg gcgacgccag aaatcggcgc aggcctggta
     8401 gcgtagcaga gactcatctt caatcagctg ctgctcaaac agcatgccgg attcaaaggc
     8461 attcaggttg agcatgcgct ggcaaaagcc gtggatagta aagactgccg cttcatccat
     8521 ctgccgctcg gctaacaaca accactgcgc ggcttgcgct ttatcgtcga tctcttccag
     8581 caggcgtttg tacagtggat tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg
     8641 gatattgcta cggatacgac cacgcaattc tgccgtggca gcctcggtaa aggtcaccac
     8701 cagcagttct tcaacggtca gcgggcgggg aaaggcggcc gaaccgccta gtccaagtaa
     8761 caggcgcaaa tagagcgccg caatcgtaaa ggttttgcct gtgccggcag aggcttcaat
     8821 caggcgctcg ccctgtaagg gcaagcgcaa aggatctagt gtctcggcga catcactcat
     8881 tcttttcact catcaggggc attgtttgct gcaacgcgct gacgttctcc cacactttcc
     8941 agccttcagg atgcacatat tcggctttcc cattctggct gccggaaatc tgcgacagaa
     9001 tagccatacc ttgcggctcg accaccgcct gatggaagaa atcagcaatt ttttgcggcg
     9061 tcagcagttt tatctgggcc acgattttat cacgcgaatc gaagcgcata ttgccgcgat
     9121 cgaaatcttt acttaacttc gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct
     9181 gggtaattac cgcctgctgg atttgcgcaa actcttctgg cttcatcgcc cgcaattttg
     9241 cctctgcggt tgggaaaaac gccttgtaac gctcccacaa gaatgaaggc tgtttatcat
     9301 tgctttgcaa aaggaagccc atgccccact gacgccccac gctcattgga aacgcaaaca
     9361 cggcatagcc caactgttct tcggtacgca actgattgta gaaccacggc tgtacgatct
     9421 gccccaacag agaactatac gctgagctgg tgtattcatc gtagccagtc ggtacaaata
     9481 ccgctgccag tgcggagtcg gtgctgttac cggctttttc aaagatgacg gattgttttt
     9541 tatcgaccac gacatctttg ttacgacacc actctgaacc atcagcgccc aactgttttt
     9601 gcacatggcg tgccagcgtt attgcctggg cttcagtcat gttgccgata accataaact
     9661 ctggtctagc ccctgatttt aaggcgtcgc gataggccag cacctctttc aacgtaatgg
     9721 agggcaaaat tttgcgccgt tcatctcgcg agaagtacgg cacttgcgag agcatctgcg
     9781 cgggcataat cgcctgctcg aacgctttgc ccttttctgc ggaatccatc atctggttat
     9841 accaggactt cgcctgctca agctgatctt ccgtagcggt atagctaaag tagccctcca
     9901 gcagagcctg gaacagctgc ggcagacgct gggtgtaacc attagcatta accataaggc
     9961 cgttgttagc attggtggaa aaacttatgc caccaaccga cgcctggttg cttaactgat
    10021 caagcgccag ccctgcgaga taatcattga gtgcaaacat cacctgattg cgggcgctgt
    10081 ccatggcttt cggattacgc aaaatcaggc tgacatcagc tttgggctcg ctggcaaaat
    10141 aacggcttgg cgcatacacc acgcgcagat tcgactcatc aacaatcaac tctggatggt
    10201 cgtatttctt ctctgactta atcagcgaga aatcgtcagg aatatagggg ttaagctccg
    10261 gtaaggagag cgcaatattg gcggcttttt tctgccagtc agcgaaagtt tgtgcgctaa
    10321 ttttatcgac ctgatacggc gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg
    10381 ggctgatata ccagatacgc gcattctgtg gcgtcatcat cgccagacgt tcttttaccg
    10441 ctttagcgtc gtaccgatcg gcaatattga ctgcatccag tgtatgctca acaggaacgc
    10501 gaatcatggt atctgccagc cattcgacgt aatccatatc acgggtgatt gacggataac
    10561 ggaagtcgat atccagcaca ttcgccagtt catcgaagta ttgtttatcg atcccttttt
    10621 cacgtaacag attgaggtag ctaaaaatgg ccgccacaac ctgatcgcga ttcgccagac
    10681 ctttatcggt taaagacgca gagatcgcta atacgccgct gttgccgttg acgataggat
    10741 cggaattggc gctaatgccc tcaactaatc cctgcttttg cagccagtca gaaagtgtac
    10801 cagggctgcg attaccaatc agataggtaa tcaattcatc cgttttacta cggaacttcg
    10861 ctgaattgtt atcgatgcga aactcaacgc gcagaacttt acgcggcagc gccgggacgt
    10921 aatgaatgat aatgcccttt tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt
    10981 tgctctcttt gttcggcacg cgaccaaagg tgtccgccgc cattttcgcc aactccggca
    11041 acggtttatt gctgtaaata accgccttca tcagattggc ggaatagtac ttctcgtgga
    11101 aatctttcag cgcctgctgt accggattac ctggtttgtc gcttaaagtt tcgaggttac
    11161 caccagaaaa ctttgaaccg gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca
    11221 tgcgcatccc gtcacgcgta cgcgccatgg ttaattcggc attcactgca ttacgctcac
    11281 gttcggcgta tttcttgtcg agcaagggtt ctgcaatagc atcggccagg cggtctaccg
    11341 caccgggtaa ggcatcgttc tcaacttcca gatagaaagc cgtgcgatac ggtgccgtgc
    11401 tggcattgtg actgccgccg tgcattttga gatattcggc cagactgtca gcctgcggat
    11461 actttttcga ccccatcaga ctcatatgtt caaggtaatg tgccagcccc tggtacgcct
    11521 caggatcttc cagcgaccca acgggcacca ccagcgccga gagcgattta actgcctgcg
    11581 gatcagaaac cagtaagacc accataccgt tatccagacg tatagcctga tactggcggt
    11641 tatctttatc acttttacgg atggtttcct gaatcggctg ccatcccgtt tctgcctgac
    11701 ttaagggtgc ccagagggca acaaacaaca ataatgcttt gaaccagatg ctgcggggca
    11761 ttcacggacc tcataatcaa ctttattttc tggccagatt caacacgtta acgcaaatca
    11821 tctgccagaa tttaatcttg tgctgaacga gtcagcctat gtctatataa ccatcagtcc
    11881 gtgactggtg cgcatcataa agtaagcgga tagattgcgc aatttttata caacactcat
    11941 gactgattaa agcgaaacag cggtaacagg aaacgttgcg actgttcaac gatagcctcc
    12001 attgtctctg gtgttaattg ccgccagagc cgttgatacc agatatcatc accttcgcca
    12061 cgcaccatca tgttgccttc gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg
    12121 gaatcgtcat ccagcatggc atcgttttgc gcgtcataac aggtttttag ccacgcgccg
    12181 ccactttcag gtaacaccag caatggcgcg gacattcctt cacgataccc ctcaatcagt
    12241 tgtgagaggt aatgcaaagc ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct
    12301 ttgcgtagaa aaaggcgact ttcaccatta ccaccgctgg cacagtagac aaggtgttcc
    12361 agccaaagtt gcattccctg cgccacactt aataaagagg gacgccagcg caacaggcca
    12421 tccggctgca cctgcggcaa ccagcccgtt atttgcacac cgttgcaggc gagatcaatc
    12481 tccatactct gccccggctg gcgacaggca atgactctgt cggcaagctg ctgcatctcc
    12541 tgacactgcg tttcccagaa aatttcacca aaagcgccat acggtaaatc ccctgccgcc
    12601 cggaagcgac ggaacaagcg ttcggcatca tcctgctcaa ccagtgcatt caataactgc
    12661 tgattgattt gataacggct aagtccttcc agaataaatg gctcggtgtc ggggatttcg
    12721 ctgtcttcgg tacggaagtt cacctgcaaa cgcatctgga aaaatgcccg caccggatgt
    12781 gcccagaatc gttgtagcgt ttccagcggc acggtttccg gtaaggtaaa tggcagcggc
    12841 tgaacaaatt cagaatgtgc tttaccagcc tggctggccg caggtagcca ttcacgagca
    12901 taactttgtc gttcgcctgg ctggtagttt tgtggatcaa acggcattcg ggtatggagg
    12961 caagtaagat gcgcttttac ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc
    13021 ggtagataat ggctttgccc gatgtagtcg atcagttcct gcaccagtac cgacgggaaa
    13081 cgttcactgt tatcctgaat ggaacgcccg atatagctga tatagagttt ttgctgcgcg
    13141 gaaattaacg cttccaggaa cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc
    13201 ttcggtttct ggctcatcag atcaaagccc aatggcgcaa gctgacgtgg ataaacgccg
    13261 tcgttcattc ccagcaggca aaccactttg aacggaattg aacgcattgg catcagagta
    13321 caaatgttaa ccggtccggc gagaaaacgc tggctgatac gttcttgatc caggcgctgt
    13381 gccagttcat cacgcaatag tgacagcggc accgcgtcgc catactgcgc acctaaacct
    13441 tcggcgataa tcgcctgcca ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca
    13501 tccggcagga agaaggcgtt gagcatatcg cgacaaaccg gcaaccactc ttccagcgga
    13561 cgctcctgcg ccagcccgcg acgccagata tttagctgca ttagcagtga agccagatgc
    13621 cccaccagtt ctgcaattaa gccgctcgat tcatcataag gtagaaccga ttgccactcg
    13681 ccctgcgcgc tctccatcgc gtagcccagc aacatgcgcg tcaggccaaa ccgccaggtg
    13741 tgttgaccgg tagcgggaag ttccagctcg cgaacgttgt cgtcatctat cccccaacga
    13801 atgccggact cgttgaccca ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac
    13861 cgcgccgcca gcaccggcac atccagtaat gccagcacgt cttccgacac aaagcggctg
    13921 tctggcaacg ataacaggct gataaacgct tcaagtacag gatgcgactg ccgcgcccga
    13981 cggtcggaaa tggcgtaagg caggtaacga tccgcaggtg cgctaccaaa cacagcctga
    14041 ataaacggac tgtagctgtc gatatcagcc accatcacga tgatgtcgcg cggagtaagt
    14101 gtcgggtctt cctccagcat cgccagcagg cgatcgtgta aaacttcaac ttcacgctgc
    14161 gggctatggc aaacgtggaa ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta
    14221 tcgctacggg aaaactcttc gatgttcaca ccagcaacgg cgcggttttc cagttccaga
    14281 atgtcagact gaatattatg cagcaggtta tctggcgtca catcgacaaa agcgtccagc
    14341 tcctggctgc tctccaggtc agaaaggaga taaatgtagt cgcgcccaag cttgccccat
    14401 gaagccagca gcgggttgcc gacatcctgt tcaccatcgc tgttaaagag ctgcccggca
    14461 ttttcgctgt cgcgaaatag cggtaattcg cgatcttcaa aactgtgtcg gcgctggcga
    14521 gtcagtagtt tcgccagata agcgggatct ttaatgtcgc cccagtaata acggcagggg
    14581 ttggtaaaca ggagatggat ttcaatatgt ttacccagcg cctgtagcgc ctggagataa
    14641 acaggcggta acgcggaaat accgcatata aagacgcgcg aaggtaaccc cggcgggcag
    14701 gtcgtcgcgg actccagcgt ttcgataaag cgctgataga gattggcgcg gtgccagcgc
    14761 ggttgcccaa gttcgtcggt atattccacc aacgccttcc acaacggcgc ttgccaggcc
    14821 tgtgcttctc ccaacccttc taccagatgt cctgtttccc actgtgccag ccagtccgga
    14881 cgatagacca gatactggtc aaacaggtcc gccgcttttg aagaaagctg gaacagtttt
    14941 cgcttgtcac tatcgtcagt cagataatgc cgcaacaggg taaagtcttc gcgctccaac
    15001 agttgcggca gcagtgtcat cagtttccag ctcatgctct gtttgttaaa ggcgctctct
    15061 ttggggatct ccggtaacac ccggacgaac atatcccaga taaagctcgc tggcagcgga
    15121 aaatcaatgt ttgccgcaat accaaacttt tgcgacaggg tcatttgcag ccactgtgcc
    15181 ataccggtac tttgcaccag aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt
    15241 tcgacaataa actccatcaa cgcttccagc acgtccagac gattggaatg gtagaccctt
    15301 aacatagcgg ctcctgacta ctgacgattc gggcaatgca ggcgggtcat ctcgccttct
    15361 ctgcccccgg gtgaaactag cgtaacgctg atgctgacac atcccgcctg cgatgtctgc
    15421 attcggttga cctgccagtt agcaggtggc gaaatcgcgc gcagttgcgt ttgctgccag
    15481 ccatgccgcc agagctgttg gtactggttt ctgctggcaa gactgttcat taatgttcgc
    15541 tggaaacccg ataacgcagt gacaatcatc accatcaaca ccatcgccaa cattacctcc
    15601 ggcagactaa agccttgttg attcctcagg gaagctgaca taacgccctc tctttcagcg
    15661 gacaaaaatc gctccagccg cgtggcgaaa aaacaatgtt tccatcgatg acttcgcctg
    15721 ttcgccacaa cgaaacgcct tcataaccag caatcaataa ggcttcatta tctgccagta
    15781 aacgcagaca aacccgggca ccggtggcag catacagtaa gcactgaact gcgggcagcg
    15841 tctgccagga gtgcattttt ccccactcca gcgccgactg aacgatggcc tggcggcgca
    15901 atgactgact ttccatgctc acgcgagaag caaaactgcg atcctgctga ctcattcctt
    15961 gcaatagcaa gctacccaaa atcagcagca tcaggaccag agccagtgac gaaacaccct
    16021 tttcgcggtt cacaggttgg atcctgtcac gctatagctg gcatccacca cggtttgcgg
    16081 atcagccttg ctggcagcgt gcatattaac cgtcaacacc ggcgagaagc cgctgacatc
    16141 ctgacgtacg acctgaaaag tgtcgataat gatggcatcc ggattagtga ctttatccca
    16201 gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg
    16261 aaatccaatc tggtcggact cttttaccgg ttcgcgatcc cagataccat tactgttcgc
    16321 atcccactgc acaatgacac agtcaccctg tccgacaatt tccagccctt cgcccgtaca
    16381 gctgccatgg caataacccg ccctctggag atgcttcgcg acggtaaata cccgcagcca
    16441 gatttcatct tccagcgcca gcttacgggt gttcgttaaa ctttcacgct gtaacgcagg
    16501 cagaaagcgt gcagccccca gcaacaatac gctactgatc gccatagcaa tcaacacttc
    16561 cagcagagaa aaaccttgct cttttacagg catccttctg tttctccttg ctgacaaagc
    16621 cggagtcttc cccacggcga aaccactagc caccactcgc ccgttgagtt tttgaagcga
    16681 atatgcccgg cccatgcggt attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg
    16741 ctcatttcga cttcgggcca gcgcggcaca aagaccaatg gtgaactgcc atgacaggta
    16801 ttcgccccag caacggaact cacaaggcac cataacgtcc cctccctgat aagactgata
    16861 ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat agagcaaata gtcccgcgcc
    16921 tggctggcgg tttgccaaag ccgttgcgac tgctgccagt attgccagcc atagagccca
    16981 cttgcgctta gcatgaccag aatcagcatc gcgaccagcg tttcaatcag cgtataacca
    17041 cgttgttttt tcatgccggc agtatggagc gaggagaaaa aaagacgagg gccagtttct
    17101 atttcttcgg cgcatcttcc ggactattta cgtcgttgca ggacgttgca aaatttcggg
    17161 aaggtgtctc gaagaattta acggagggca aaaaaaaccg acgcacactg gcgtcggctc
    17221 tggcaggatg tttcgtaatt agatagccac cggcgcttta atgcccggat gtggatcgta
    17281 tccttcaatc tcaaagtctt cgaaacggta gtcgaagatg gattcgggtt tacgtttgat
    17341 aatcaacttc ggcagcggac gcggttcgcg gcttaattgc agatgagttt gatccatatg
    17401 gttgctgtac agatgcgtgt cgccaccggt ccagacaaaa tcacccactt ccagatcgca
    17461 ctgctgcgcc atcatatgca ccagtaacgc gtagctggca atgttgaacg gcaggccgag
    17521 gaagacgtca caggagcgct gataaagctg gcaagagagt ttgccgtctg ccacatagaa
    17581 ctggaagaat gcatggcacg gtgccagcgc cattttatcc agttcgccta cgttccacgc
    17641 tgaaacaata atgcgacggg aatccgggtc gtttttcagc tggttcagta ccgtagtgat
    17701 ctggtcaata tgacgaccat ccggcgttgg ccaggcgcgc cactgtttac catacactgg
    17761 cccgaggtcg ccgttttcat cggcccattc gtcccagatg gtgacattgt tttcgtgtag
    17821 ataagcaatg ttagtgtcgc cctgcaggaa ccacagcagt tcgtggatga tggaacgcag
    17881 gtggcaacgt ttagttgtca ccagcgggaa tccatcctgc aggttaaaac gcatctgatg
    17941 accaaaaatg gaaagcgttc cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag
    18001 cactttttgc atcagttcta aatactgttt catggttcct caggaaacgt gttgctgtgg
    18061 gctgcgacga tatgcccaga ccatcatgat cacacccgcg acaatcatcg ggatggaaag
    18121 aatttgcccc atgctgatgt actgcaccca ggcaccagta aactgcgcgt cgggctggcg
    18181 gaaaaactca acaatgatgc gaaacgctcc gtaaccaatc aggaacaagc ctgagacagc
    18241 tcccattggg cgcggtttac gaatatacag gttgaggata ataaacagca ccacaccttc
    18301 cagcagcagc tcgtaaagct gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat
    18361 ggattgccac tgcgggttgg tttgcagcag caaaatatct tctgtacggg agccagggaa
    18421 cagcatggca aacgggaagt tcgggtcaac gcggccccac aattcaccgt taataaagtt
    18481 gcccagacgc ccggcaccaa gaccaaacgg aatgagtgga gcaataaaat cagagacctg
    18541 gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc accacgataa cgccaatcag
    18601 gccgccgtgg aaggacatgc cgccgtccca gacacggaaa agatacagcg gatcggccat
    18661 aaactgcggg aaattgtaga acagaacata accaatacgt ccaccgagga agacgccgag
    18721 gaagcccgca tagagtaagt tttcaacttc atttttggtc cagccactgc ccggacgatt
    18781 cgcccgtcgt gttgccagcc acattgcaaa aatgaaaccc accagataca tcagtccgta
    18841 ccagtgaagc gccacgggtc ctattgagaa aatgaccgga tcaaactccg gaaaatgcag
    18901 atagctactg gtcatctgtc accacaagtt cttgttattt cgctgaaaga gaacagcgat
    18961 tgaaatgcgc gccgcaggtt tcaggcgctc caaaggtgcg aataatagca caaggggacc
    19021 tggctggttg ccggataccg ttaaaagata tgtatatgat ccgcgctata accccccacg
    19081 aatcagcccg cccatgccgc gacgctccat aaaggctgca acctgatggc gaacttcggt
    19141 cgccagttgc gcttccagac tacgctgcgc aagattttct gcttcggcaa aatcaatgcg
    19201 ccgcagcagg tattttacgc gcgctacaga acgtccgttc atagacagat ggcgataccc
    19261 aagcccaatg aggattgcca cgcacatcgg atcgcccgcc atttcaccgc acaaacggag
    19321 atcgattcca tgtatttccg cttcccgggc gatcatcgcc agagctcgta acattgcagg
    19381 atgaagactg tcataaatgt tcgccacccg ggtattgttg cgatcaacgg ccaggatgta
    19441 ttgcgtcaga tcgttggtgc caacagagat gaaatcgacc cgctttgcca gatgcggcag
    19501 cataaacacc attgacggca cttccagcat gatgccgata cgtggtttgg gaatttcgta
    19561 accgatcatc tcctcgactt cacgtccggc acgttcaatc aggcggcgtg cttcgtcaac
    19621 ttcatcgagg cttgtgacca tcggcaacag aatattcagg ttgcccgtag cggcattagc
    19681 acgcagcatc gcccgcacct ggatcaagaa gatctccggc tgatcgagcg taatgcgaat
    19741 cccacgccaa cccaggcatg gattctcttc gctgatcggc atgtaaggca gctgcttatc
    19801 tgctccgaca tccagcgtac gcaaggtgac gggtttatca ttaaacattt gcagcatccc
    19861 ctgatactgc gccacctgtt cttcttccga cggaaaacca ctttgcagca tgaatgggat
    19921 ttcagtgcga taaagtccta tgccatcaat acggctgccc agtttttctt catgttccgg
    19981 gcttaaaccg gcattgagca tgactttaat gcgttcgccg ctttttaact gggcgggtaa
    20041 attgacgtca tcttccgcca gacggctaag ctcgatctct tcactaatta gccgctgata
    20101 ttcttgcagc agtaccggct ccggatcgac cagcaattca ccgcgataac catcgacgat
    20161 cagcgtccga cgatgcagta ccgaaggctg aatatccgcg cccatcacgg tagggatccc
    20221 cagcgcacgt accatgatcg cagcatggga gttggcagcg ccatcgcgca cgacaacacc
    20281 gactaagcga tcctggggca gctcagcaag cgttgtcgct gacagttcat ctgccaccag
    20341 aatgaaacgt tccggccagg cgttcggccc ttgattagcg tcatcaagat gaaacagcaa
    20401 tcgctgacca agtgcacgta aatcgccagc ccgctctttg agatagttat cgcttagcgc
    20461 ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc gcccactctg ccaccgagcc
    20521 tttatcaacc tcggcaaaca attcgcgacg cagccgggta tccgaaagca ggtgtgagta
    20581 aagatcgaaa atagccgccg tttctttttg tgcaccggca gcaaagcgtt tgctatagcg
    20641 gcgaaactcg ttagccgcct cttccaacgc cccggtcagt cgttcgcgtt ccagagccgg
    20701 atccagcgtt gatgcctgat acacctgttc cattaaaggt aacgtggcat cctgccagcc
    20761 ttcggcaatc gccacaccag gtgctgccgg taatgcacgg attcgcgtct ggcgatattg
    20821 cccaaacaag gcagtcaact gcgactgaga aagaatagct gccatctggg tggcaagcgt
    20881 caccaggaag gattcttcac tttcgtcata ctggcgcaac tctcgttgct gtaccaccag
    20941 tacaccaagc aactggcgac gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc
    21001 ttcttttacg gaggggatgt atttgaagct ggggtgcttt tgcgcatctg caaggtttat
    21061 cggttccgcc agcctgccaa ccaggccgac gatcccttca tcaaacgcga gcgttacagt
    21121 gcgaccgcgt ggttttttca gtccccgggt cgccatcagg tagtaacaac gtcgatcatg
    21181 atcggccagg tagaccgaac agacctcggt atccatcgca agacagatgt cggtaaccag
    21241 aatatttaac gcctcattca ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag
    21301 gcgagtgagc ataattggcg tgacttaacc tcttttacgt cgataagcag atgcgttttg
    21361 tggttttggc gtattttcct gcagtgacat caccacactc gcgaactctt tcattaccct
    21421 acggtagaca tcacgtttaa atgacaccac ctgtctgact ggataccagt aacttaccca
    21481 tcgccagccg tcaaactctg gtgtactgct ggtttgcata ttgatttctg catcgccgct
    21541 caccagctgc aagagaaacc atttttgttt ttggccgata caaaccggct tcgtgtccca
    21601 acgcaccaaa cgtttcggta atttgtagcg caaccagtta cgcgttgaag caaggattcg
    21661 aacgtctttg cggcttaatc ctacttcttc aaacaattca cggtacatcg cctgctctgc
    21721 ggattctccg gggttgattc cgccttgcgg aaattgccag gagtgctgac caaatcgccg
    21781 ggcccacatt acctgcccct ggcgattaca aatcacgata cctacgtttg ggcggtagcc
    21841 atcgtcatca atcaccggac tacctcaaaa taaagcttta tatacgaatg attgtttcat
    21901 actccaggaa gacggtaaac cactctctgc agggcattac acactaataa caattgaata
    21961 actcacagtt atgtgcagag ttataaacag aggaaggggt ggatagccgt ttttattcac
    22021 cttttctgtg gatagagttg tgaagaacta cg
//
LOCUS       DACADA010000071        21317 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.072, whole genome shotgun sequence.
ACCESSION   DACADA010000071 DACADA010000000
VERSION     DACADA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.072"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(191..1102)
                     /gene="gsiD"
                     /locus_tag="ISB17_002620"
     CDS             complement(191..1102)
                     /gene="gsiD"
                     /locus_tag="ISB17_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAP1980536.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1105..2025)
                     /gene="gsiC"
                     /locus_tag="ISB17_002621"
     CDS             complement(1105..2025)
                     /gene="gsiC"
                     /locus_tag="ISB17_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAP1980537.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGILLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2043..3581)
                     /gene="gsiB"
                     /locus_tag="ISB17_002622"
     CDS             complement(2043..3581)
                     /gene="gsiB"
                     /locus_tag="ISB17_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAP1980538.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3601..5472)
                     /gene="gsiA"
                     /locus_tag="ISB17_002623"
     CDS             complement(3601..5472)
                     /gene="gsiA"
                     /locus_tag="ISB17_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAP1980539.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5459..6424)
                     /gene="iaaA"
                     /locus_tag="ISB17_002624"
     CDS             complement(5459..6424)
                     /gene="iaaA"
                     /locus_tag="ISB17_002624"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP1980540.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACLMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6628..7863
                     /gene="moeA"
                     /locus_tag="ISB17_002625"
     CDS             6628..7863
                     /gene="moeA"
                     /locus_tag="ISB17_002625"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAP1980541.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7863..8612
                     /gene="moeB"
                     /locus_tag="ISB17_002626"
     CDS             7863..8612
                     /gene="moeB"
                     /locus_tag="ISB17_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAP1980542.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8688..9350)
                     /gene="fsa"
                     /locus_tag="ISB17_002627"
     CDS             complement(8688..9350)
                     /gene="fsa"
                     /locus_tag="ISB17_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP1980543.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAVGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9481..10380
                     /locus_tag="ISB17_002628"
     CDS             9481..10380
                     /locus_tag="ISB17_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAP1980544.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10386..12818
                     /locus_tag="ISB17_002629"
     CDS             10386..12818
                     /locus_tag="ISB17_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAP1980545.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12964..13779
                     /gene="ybiV"
                     /locus_tag="ISB17_002630"
     CDS             12964..13779
                     /gene="ybiV"
                     /locus_tag="ISB17_002630"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAP1980546.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHITLDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(13979..15571)
                     /locus_tag="ISB17_002631"
     CDS             complement(13979..15571)
                     /locus_tag="ISB17_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAP1980547.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            15790..16710
                     /gene="ldtB"
                     /locus_tag="ISB17_002632"
     CDS             15790..16710
                     /gene="ldtB"
                     /locus_tag="ISB17_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1980548.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16768..17886)
                     /locus_tag="ISB17_002633"
     CDS             complement(16768..17886)
                     /locus_tag="ISB17_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAP1980549.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(17883..18350)
                     /locus_tag="ISB17_002634"
     CDS             complement(17883..18350)
                     /locus_tag="ISB17_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAP1980550.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18536..18664
                     /gene="mntS"
                     /locus_tag="ISB17_002635"
     CDS             18536..18664
                     /gene="mntS"
                     /locus_tag="ISB17_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAP1980551.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18936..20519
                     /locus_tag="ISB17_002636"
     CDS             18936..20519
                     /locus_tag="ISB17_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP1980552.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20568..21083)
                     /gene="ompX"
                     /locus_tag="ISB17_002637"
     CDS             complement(20568..21083)
                     /gene="ompX"
                     /locus_tag="ISB17_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAP1980553.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21136..21201)
                     /locus_tag="ISB17_002638"
     CDS             complement(21136..21201)
                     /locus_tag="ISB17_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980554.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
BASE COUNT         5280 a         5864 c         5227 g         4946 t
ORIGIN      
        1 tataaactca gcatgttaat acatttaaaa aaataactgt ctgaacgctg tgccgctgca
       61 aaatagtccc ttgctgcggc tttttaacca aattacccga ttctacaaaa tcagcagaaa
      121 gttacaacac ttgcttaggg caataatttc ctgtgaggtg attacccttt caagcaatat
      181 tcaaacgtaa ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca
      241 aattgaacgc cagtacggtc agaaaaatag ccagggccgg aaatacagcg acatgcggcg
      301 cgataaccat atccgctcgt gcctcattga gcattgcacc ccactctggt gtcggcggct
      361 gcgcaccgag gccgagaaat gacaggctgg cggcagagat aatcgaggtg ccaatgcgca
      421 tggtgaaaaa caccacgata gaagagacgg tcccaggcaa gatatgacgc aacaaaatgg
      481 tcatatcgct ggcaccaata ctgcgtgctg actcgataaa ggtttgctgt ttcaacacca
      541 gcgtgttgcc gcgcaccagc cgggcaaacg cggggatgga aaaaatggcg actgcgataa
      601 tcacgttagc aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg
      661 ggaaggcaaa cagcacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat
      721 atccagccag caagcccagc aacgtcccga tcgccgcacc gataaacacg gcaaacacgc
      781 ccgccgccag cgagatttgc gcaccaacca ggacacggct gaaaatgtcg cgccccagtg
      841 aatcgacacc aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat
      901 aattttcggc gtcataggga gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa
      961 taacgaacaa tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat
     1021 gccacggtgt acgtacctgg tcaggtttga ccagtggcat ggcgtttaac accgcctgac
     1081 gtcgccagtt aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta
     1141 aagcacatcc accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc
     1201 ctgaatcact gggtaatcgc gcatttctac ggagtcaacg agtaagcgtc caagtccggg
     1261 ccagttgaaa actttctcca ccacgatgga accaccgagc aagaagccaa actgtaagcc
     1321 catcatggtc actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt
     1381 ttcgctcacc cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa
     1441 cgacgcgcgg gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg
     1501 taaaatgtag tgctgccaac tgtctgctcc cacggtaggc agccagccca gttcaacgga
     1561 gaatacctga attaaaagta tccccagagc aaatgccgga aacgagatcc ccgacaccgc
     1621 aatggtcata ctcaatcgat ccggccaacg gttacgccag acggcggcga taattcccgc
     1681 cgccatacca aatatcaccg cccagaccat actggttatg gtcagccaga gcgttggcat
     1741 aaagcggctg gcaatctcat cggcaaccgg acgacgcgac accatcgaca ggccaaaatc
     1801 cccctgcaca gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc
     1861 caactgctga cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caatcaatcg
     1921 cgccggatcg ccgggcagca tatggacaaa taaaaacacc agcaccgaga cgataaacag
     1981 cgtcggaatc aaccccagta agcgtttgat aacgtaatta agcattccac tccctgcgtt
     2041 gcttattgca aatccgcgtc ttcaaagctg aagccggtgt ctggcatgat ccaaaaaccg
     2101 gtcaggtttt tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgac
     2161 tcttgccaga tgatatcctg cgccgcctta tacaagcggg tcttttccgc cggatcatta
     2221 gttttcagtg cctgagccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta
     2281 aacagcgttg gtggccagtt ctgcgaggca aacagcggcg atagagccca gtcggcttcg
     2341 ccggttgaag ccgaccagcc agtgtagaac atccgcacgc cgctctcttt ttgcccttta
     2401 ccttcaactt ctgccgcccg ctgtccggca tccatcgcag tcacctgggc tttaatccca
     2461 acctgcgcta actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt
     2521 gacgaccaca gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc
     2581 gctttcactg gatcgtaagg ccacggttta taactttgcg cgtaggcgat actcggcggt
     2641 accacaccag tagctggcgt tgcatagccc gcgaaggcca ctttcaccag cgccgggcgg
     2701 ttaatggcgt aattcagcgc ctcacggacc ttcgggttat cgaacggctt ttgcgtcacg
     2761 ttcatactga tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt
     2821 ttctccagca gtgcggcctg ctcgtaggga atggggaaag caaactgcgc ttcaccggtt
     2881 tgcagcattg ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt
     2941 ttgggcaatc ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga
     3001 ttccaggtat ccagttcata cggcccggtt cccaccggat gaaaaccaat ctccttgcca
     3061 tatttttcca gcgctgccgg tgatatcatc gcggtcgccg gatgggcaag aatattaata
     3121 aacgctgaga atggctgttt gagggtaatc tttaccgttg tcggatcgat cgcttccgtt
     3181 ttggcgatat tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg
     3241 tccagattcg ctttcaccgc cgctgcgttg aaatcggtgc catcctggaa tttaatgcct
     3301 tcccgcaatt tcacggtgta agtaaggccg tcatcggaaa cggtgtaact ctccgccagc
     3361 acgtttttca gtttcatctc tttatccaga ccgaacagcc cctggtaaaa cgatttcgct
     3421 acggcctgag ataacgtgtc atttgcgtca tacggatcga gcgtggtgaa gttcgatccc
     3481 accgccacca ccacctcttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg
     3541 cccagcgcca ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg
     3601 ttatctacgc atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac
     3661 gcattgcaac gagacggctg caacctcttc gccacgcaga tgaatattgc tgggaagatc
     3721 gtccgacagc agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc
     3781 cagtaattta cgcgtataag gatgctgcgg attttcaaag accgcgcgcc gtggaccaat
     3841 ttcaacaatt tgcccgagat acatcaccgc cacacgatga ctaatccgct caaccacggc
     3901 catatcgtgg gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa
     3961 gttgataatc tgcccgcgaa tagaaacatc cagcgccgag acggcttcgt cggcaatgat
     4021 cacttttgga ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga
     4081 aaactcatgt gggtaacgcc aggcatgttc cggtaacagg cccacgcgct ccagcaacca
     4141 cgcaacgcgt gcagccgctt ctttacctgg caataaaccg tgtacgcgca gcggttcgag
     4201 aatcgaatca ccgatagtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa
     4261 ctgaatatcg cggcgcaatg cctgaagttt accgggtgac aaggtatcga ttcgctgacc
     4321 gttaaagata atttcgccgc cctgcgattc gaccaggcgc aacaacgccc gcccggtagt
     4381 ggatttaccg ctgccagact cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa
     4441 actgactttc tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa
     4501 agggaaacgg gtgaccagat tacgcacccg taaaacaggt tcgccatcca ccaccgtttt
     4561 ctgctcgatg gatgactcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg
     4621 gggataatct aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt
     4681 ataaggatgt tgcggtgcat gaaaaatctg ttcgacgcta cccgtttcca ccgcctcgcc
     4741 ctgatacatc accagtaccc gatcggcaat ctctgccacc acacccatat cgtgagtgat
     4801 aaagataacg cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc
     4861 ctgaatagtg acatccagcg cggtggttgg ctcatcggca atcagcaccg ccgggcggca
     4921 tgacagcgcc atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcggata
     4981 acgtgaaaga atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc
     5041 gaccatcgct tcttcacgac tggcgttctg atgcagacga atagattcag caatctgttc
     5101 acccacagta aataccgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc
     5161 cgcaccgcgc acatggcgca tttgtgcagc gctctgctcg ctaagttcaa ttacttcgcg
     5221 actgcgccgc tgcaacagca ttttatcgca ctgcactaaa ccgcccgcct gttccaacag
     5281 gcgcatcaat gccagcgcag tcactgactt accggagccg gattcgccaa caattgccag
     5341 cgtctcacca cgttgcaggc taaaagagag attgcggacc gcagctattt tctgctgctc
     5401 ctgcataaag gcaatattca gattttcaac cgccagcaca tcaccggcat caagttcatc
     5461 actgtgtggc aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg
     5521 cgtagcccca ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat
     5581 ggtcgatagc gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc
     5641 gctcgcaggc ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atatcatatg
     5701 ccgccagcgc gcggatgaag acttcgcccg taccggtaca ggaaaccgcc acactggcgt
     5761 tattggcata gcatccggcc cccactaagg gactatcgcc aactcgtccg ggtaatttat
     5821 tggtcattcc gcccgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg
     5881 cccctacggt gcccattttc tgtttttcat ccagtggcgc accgctatgg tcgaggactg
     5941 ttgccccttc ctcgcgcgct gccattagtt gttcataacg caaaggtgtg gagaaaatct
     6001 ccggcgagac gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca
     6061 tcatcacatg cgggctttgc tccatcacca gccgggcggc aagaaccgga ttacgcagat
     6121 gactaacgcc cgccaccgca ccggctttca gggtgttacc atccatcaga caggcgtcca
     6181 gttcatgggt ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac agtggacact
     6241 cttccagcag acgcaccgct tccgtcacca catccagcgc actttcgccc gctaccagca
     6301 ttttctgccc ggtttcaaca atggcagaca acgcctcgat gtagcgtaat tcctgttgca
     6361 gactcatctg cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc
     6421 ccatagtatt cgtccattaa gagtggctcg ccacttttgc tataaatatc agaattttta
     6481 tgaatgactc attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggagc
     6541 cagcgctacg cccgaggtgg catacagtct gtggcgtttt tctgacataa taggcaaatt
     6601 cgattttgcc tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac
     6661 accgcgctta atgagatgct ttctcgcgtt accccactga ccgcccagga aacgctgcca
     6721 ctggttcagt gttttggccg tattctggcg agcgatgtcg tttcgccact ggatgtcccg
     6781 gggtttgata actccgctat ggacggctac gcggtgcgtt tagccgatat tgcctccggg
     6841 caaccgctgc ccgttgctgg taaatccttt gccggtcagc cgtaccatgg tgaatggcct
     6901 gcgggtacct gcattcgtat tatgaccggt gcgccggtgc cggaaggctg cgaagcggtg
     6961 gtgatgcagg agcagactga acaaacggac aatggcgtgc gttttactgc tgaagcgcgt
     7021 agcgggcaaa atattcgccg tcgcggtgaa gatatctctg caggtgcggt tgttttcccg
     7081 gcggggactc gcctgactac cgctgaactg ccagtgattg cttcgctagg gattgccgaa
     7141 gttccggtga ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg
     7201 cccggtcagc cgctgggcga cggccaaatc tacgatacca accgtctcgc cgtacacctg
     7261 atgttagaac agttgggatg cgaggtaatc aacttaggga ttatccgcga cgatccccat
     7321 gccctgcgcg ccgcatttat tgaagccgac agccaggcgg atgtggtgat cagttccggc
     7381 ggtgtttcag tgggtgaagc ggattacacc aaaacgattc ttgaagagct gggggagatc
     7441 gccttctgga aactggcgat taaaccgggt aaaccgttcg cgttcggtaa actttctaat
     7501 agttggttct gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg
     7561 gtacagcctt tgctggcaaa actaagcggc aataccgcca gcggcctgcc cgcgcgccag
     7621 cgtgtacgca cggcatcgcc tctgaagaaa tcgccaggac gccttgattt ccagcgcggc
     7681 gtgctgcaac gcaacgccga tggcgaactg gaagtgacga ccaccggaca tcagggttca
     7741 catatattta gctcctttag cctcggcaac tgctttatcg tgctggaacg cgatcgcggc
     7801 aatgtggaag tgggcgaatg ggtggaagta gaaccgttta acgcgttgtt cggaggcctg
     7861 taatggcgga actcagcgat caggagatgc tgcgctacaa ccggcaaatc attctgcgcg
     7921 gttttgattt tgacggtcag gaagcattga aagattctcg cgtgctggtc gtcggccttg
     7981 gtggactcgg ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc
     8041 tgctcgactt cgacacggtt tcgctctcca atctgcaacg ccagacgctg cacagtgatg
     8101 ccacggtcgg gcaaccaaag gtggaatccg cccgtgacgc cctgacgcgg atcaacccac
     8161 atatcgcgat tacgccagtc aatgcgctgc ttgatgacgc tgaacttgct gcgatgattg
     8221 caaaacacga tctggtgctc gactgtacgg ataacgttgc agtacgtaat caactgaacg
     8281 caggctgttt tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaagggc
     8341 aaatcacggt cttcacttat caggacggcg aaccgtgcta tcgctgcctt agccgtttgt
     8401 ttggcgagaa cgcgttaacc tgcgtagaag caggcgtgat ggcaccgtta atcggcgtaa
     8461 ttggttcgtt gcaggcaatg gaagcgatca aactgctggc aggttatggc aaaccagcca
     8521 gcgggaaaat cgtgatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc
     8581 gcaatccggg gtgtgaggtg tgcgggcagt aactgccacc tccaggccag ataagacgcg
     8641 gcactccgcc acgtcatcag caatttatga ggatgcagaa cgtccggtta aatcgacgtt
     8701 ctgccaaacg ctccctgcca gtcctgctca aacttcgcca cagcggcatc aaccgccgga
     8761 tagctaatca tctgttgtgc cacatccagt ggcagagtaa ttgattcaca ccctgccagt
     8821 aagcagtcca gcgcctgacg cggggttttg aaactcgcgg ctaacacttt cgcctgcggc
     8881 gcatgcattt tcaataactg gtgtaagtcg gtcacagtct gaataccgct accgccctga
     8941 gcatcaatac gattaacgta aggcgcaaca tattccgcac ctgccagcgc cgacagtagc
     9001 ccttgtgctg cgccatatac cgcggttccc agcgtcggaa tcccttccgc ttttaacatc
     9061 ttaatagctg ccagcccctc tgcggtcacc ggaactttca ccacgatatc cgcaataata
     9121 gaacgcaatt tacgtgcgtc attcaccatc ccttcagcag tggtagccat tacctgggca
     9181 aacagacgcc cctgaccgcc catcgcctcg tgaagttgcg gaagcacaac ctccagcggt
     9241 tttttaccca ctgcgataat gcttgggtta gtggttacac ccgccagcgg aaaaatacgt
     9301 gacagcgcct tcaccgcaac aacgtctgaa gtatccagat acagttccat aaccatcctc
     9361 aaaatgtact ttttgtctga atgtagacta tcacgggaaa actctctgaa cagttgacgc
     9421 acatcaatcc aactttcatt cgaaagtatt ttaatgtttg aacgaaacag agaggcaact
     9481 atgattttca atattcaacg ctactcgacc catgatggcc ccggtatccg cacggtcgta
     9541 tttcttaaag gctgttcgct gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc
     9601 acgcaggatc tgctgtatga cccacgactc tgtctggaag gctgtgagct gtgcgctaag
     9661 accgcaccgg aagtgattga gcgcgcgttg aatggtttgc ttattcatcg ggaaaagtta
     9721 accccggagc attttacggc gctaaccgac tgctgcccga cacaggcgtt aaccgtgtgt
     9781 ggtgaagtga aaagcgttga ggagatcatg gcaaccgttt tgcgcgataa accgttttac
     9841 gatcgcagcg gcggcggctt aacactttct ggcggagaac cctttatgca gccggaaatg
     9901 gcgacagcgc tactgcaagc cagccacgaa gccggtattc atactgcggt agaaacctgt
     9961 ctgcatgtgc cgtggaaata tatcgcccct tctctgtcct atattgatct gtttcttgcc
    10021 gatttaaaac acgttgccga cgcgccgttt aaacagtgga ccgacggtaa cgccgccagg
    10081 gtgctggata atctgaaaaa actcgccgca gcgggcaaaa aaatcattat ccgcgtgcca
    10141 ctgattcagg gctttaatgc cgacgaaacc tctgtgaaag ccattaccga ttttgccgcc
    10201 gacgagctgc acgttagcga aattcatttc ctgccctacc acacgctggg catcaacaaa
    10261 tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc gccagaactg
    10321 ctcgactttg cccagcagta tgcctgccag aaagggttaa ccgcgacctt acgaggataa
    10381 caaccatgac cacactgaaa ctggacacgc tcagcgatcg cattaaagcg cacaaaaatg
    10441 cgctggtgca tattgtgaaa ccgccggtct gtaccgagcg cgcgcagcac tataccgaga
    10501 tgtatcaaca acatctcgat aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc
    10561 acctggcgaa tcgcaccatc tggatcaaac atgatgaatt gatcattggc aaccaggcaa
    10621 gcgaagttcg cgccgcgccg atcttcccgg aatataccgt ctcctggatc gaaaaagaga
    10681 ttgatgatct ggcagatcgt cccggtgctg gttttgcggt gagtgaagag aacaaacgcg
    10741 ttctgcatga agtgtgcccg tggtggcgcg gtcagaccgt acaggatcgc tgctacggca
    10801 tgtttaccga tgagcaaaaa ggtctgctgg cgaccggcat cattaaagcg gagggcaata
    10861 tgacctccgg cgatgcgcac ctggcggtca atttcccgct actgctggaa aaaggtcttg
    10921 atggtctgcg tgagaaagtg gcggaacgtc gctcgcgcat caatctgacg gttctggaag
    10981 atctgcacgg cgagcaattc ctgaaagcga ttgatatcgt gctggtggca gtcagtgaac
    11041 acattgaacg tttcgctgcc ctggcgcgtg aaatggccgc gaccgaaacc cgcgaaagcc
    11101 gtcgcgatga actgctgacg atagcagaaa actgcgatct tatcgcccac cagccgccgc
    11161 agactttctg gcaggcgctg caactgtgtt acttcatcca gttgattttg cagattgaat
    11221 ctaacggtca ctcggtatcg tttggtcgta tggaccagta tctctatcca tactatcgcc
    11281 gtgacgttga actgaaccag acactggatc gcgaacacgc catcgagatg ctgcatagct
    11341 gctggttgaa attgctggaa gtgaacaaaa tccgctccgg ctcacactca aaagcctctg
    11401 cgggaagtcc gctgtatcag aacgtcacca ttggcgggca aaatctggtt gatggtcaac
    11461 cgatggacgc ggtaaatcca ctctcttatg cgatcctcga atcttgcggt cgcctgcgtt
    11521 cgactcagcc taacctcagc gtgcgttacc atgcgggtat gagtaacgat ttcctcgacg
    11581 cctgtgttca ggtgatccgc tgcggcttcg ggatgccggc gttcaacaac gacgaaatcg
    11641 tcatcccgga atttattaaa ctcggtattg aaccgcagga cgcttacgac tacgcagcaa
    11701 ttggttgtat cgaaaccgcc gtcggtggca aatggggcta tcgctgtacc ggcatgagct
    11761 ttatcaactt cgcccgcgtg atgctggcgg cgctggaagg cggtcgtgat gccaccagcg
    11821 gcaaagtgtt cctgccacaa gaaaaagcgt tgtcagcggg taacttcaac aacttcgatg
    11881 aagtaatgga cgcatgggat acgcaaatcc gttactacac gcgtaaatca atcgaaatcg
    11941 aatatgtcgt cgacaccatg ctggaagaga acgtacacga tattctctgc tcggcgctgg
    12001 tcgatgactg tattgagcga gcgaaaagta ttaagcaagg cggcgcgaaa tatgactggg
    12061 tttctggcct gcaggtcggc attgctaacc tcggcaacag cctggcggca gtgaagaaac
    12121 tggtatttga acagggcgcg attggtcagc aacagctggc tgccgcactg gcggatgact
    12181 tcgacggtct gactcacgag cagctgcgtc agcggctgat taacggtgcg cctaagtacg
    12241 gcaacgacga tgatactgtc gatacgctgc tggctcgcgc ttatcagacc tatatcgacg
    12301 aactaaaaca gtaccataac ccacgctacg gtcgtggtcc ggttggcggc aactattacg
    12361 cgggtacgtc gtctatctcc gctaacgtac cctttggcgc gcagactatg gcaacaccgg
    12421 acgggcgtaa agcgcatacc ccgctggcag aaggcgctag tccggcctcc ggtactgacc
    12481 atctcggccc aacggcggtc attggctcag taggtaaact gcctacggcc gcgattctcg
    12541 gcggcgtgct gctcaaccag aaactgaatc cagcaacgct ggagaacgaa tctgacaagc
    12601 agaaactgat gatcctgctg cgcaccttct ttgaagtgca taaaggctgg catattcagt
    12661 acaacatcgt ttcccgcgag acgctgctgg aggcgaaaaa acatccggat cagtatcgcg
    12721 atctggtagt gcgcgtcgcg ggctattccg ccttcttcac cgcgctctct ccagacgctc
    12781 aggacgatat catcgcccgt actgaacata tgctgtaatc ttctcagccc ggcggcggcg
    12841 tcgccgggct aaatcattca catcatcaat ttcagcctcg ctttcattcg aaatataatt
    12901 tgtgctctgc gtcacattgt tattacactg ttacggtttc attttcgagc caggagcaca
    12961 cctatgagcg taaaagttat cgtcacagac atggacggta cttttcttaa cgacgccaaa
    13021 acgtacaacc aaccacgttt tatggcgcaa tatcaggaac tgaaaaagcg cggcattgag
    13081 ttcgttgtcg ccagcggtaa tcagtattac cagcttattt cgttctttcc tgagctaaag
    13141 gatgagatct cttttgtcgc ggaaaacggc gcactggttt acgaacatgg caagcagttg
    13201 ttccacggcg aactgacccg acatgaatcg cggattgtta ttggcgagtt gctaaaagat
    13261 aagcaactca attttgtcgc ctgcggtctg caaagtgcat atgtcagcga aaatgccccc
    13321 gaagcattcg tcgcactgat ggcaaaacac taccatcgcc tgaaacctgt aaaagattat
    13381 caggagattg acgacgtact gttcaagttt tcgctcaacc tgccggatga acaaatcccg
    13441 ttagtgatcg acaaactgca cataacgctg gatggcatta tgaaacccgt caccagtggt
    13501 tttggcttta tcgacttgat tattcccggt ctacataaag caaacggtat ttcgcggtta
    13561 ctgaaacgct gggatctgtc accgcaaaat gtggtagcga ttggcgacag cggtaacgat
    13621 gcggagatgc tgaaaatggc gcgttattcc tttgcgatga gcaatgctgc ggaaaacatt
    13681 aaacaaatcg cccgttacgc taccgatgat aataatcatg aaggcgcgct gaatgtgatt
    13741 caggcggtgc tggaaaataa accgccgttt aatctttgat tatcaccaat tcagtttatt
    13801 gagaaagtgc atgtcattat accggatacg gcgtgaccgc cttatttggc atacaaaatc
    13861 atgaaaattc aatatattgc gatacactac caggcctgat aagcgtagcg caccaggcaa
    13921 tcttgcgttt gtcatcagtt tcagtagccc tgaccatatc agggctactg ataataactt
    13981 actcgatccc tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt
    14041 ccggggtgat ttccagaata cgggtcgcca gggagcttac aaactcacgg tcgtgagaaa
    14101 caaagatcag cgtgccctga tacagttcca gcgccatgtt cagcgactca atggattcca
    14161 tatccaggtg gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact
    14221 taccaaacag catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt
    14281 cgtcctggct gaacagcaaa cgaccgagaa tactgcgcac cgcctgctcg tcatcgcctt
    14341 cctgcttcca ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt
    14401 ggtcctgagc gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg
    14461 gttgcagatc gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca
    14521 gtaccgccag tttttcaccc acttccagca gcaggttgag atttttaaac agtggaccgt
    14581 tatcaaaccc tttagtcaga ccttccactt ctagcgcgtt acggaacagt ttcttatcct
    14641 gttcaaaacg gatgaacggg ttctgacggc tggaagcttt cacctcttcc agtttgattt
    14701 tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc
    14761 ggctaacgaa agattgcaac tcagcaatct gcgctttctt cttcgcgtta tcagccagca
    14821 gacgttcacg cgcctgagtc gccgccgtca tgtactcatc gtagttgccc ggataaacgc
    14881 gcagctcgcc gtaatccaga tccgccatgt gggtacaaac catgttaagg aagtgacggt
    14941 cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa
    15001 tggtgtcgat atcgaggttg ttggtcggtt cgtcgagcag gagaatgtcg ggatcagcaa
    15061 acagcgcctg agccagcagc acacgcagct tccagccagg agcaacttca ctcatcgggc
    15121 cgtagtgctg ttccactgga attcccacgc caagcaacag ttcaccagcg cgagcttccg
    15181 cagagtaacc gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt
    15241 cttcactcat ttccggcaaa gcatagatgc ggtcacgctc ctgcttcact tcccacaact
    15301 ctttatgccc catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct
    15361 gacgcagctt accaatgcgt tcgttgggat cgagggaaac gttacccaac gtcggctcta
    15421 agtcgccgcc gaggatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc
    15481 cgtaacggtt gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga
    15541 actgcatggt gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa
    15601 ccgcgcatta tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca
    15661 agccaaaggc aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac
    15721 ttataatgcg cttccagtac tctaatattc tcaacccaat ggcctgccag gcacaaaatc
    15781 tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc
    15841 ttttgcagta ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt
    15901 ggtcagaatc aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc
    15961 gccgagtacc agatgggact ttccaatatg atggaagcga acccgggtgt ggataccttc
    16021 ctgccgaaag gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt
    16081 catgaaggca tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc
    16141 aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag gcaaagacac gcctatcaac
    16201 tggaccacca aagttgagcg taaaaaagca ggcccgacct ggacgccgac cgctaaaatg
    16261 cacgcagagt accgcgctgc gggcgaaccg cttccggcgg tcgttccggc tggtccggat
    16321 aacccgatgg ggctgtatgc actctacatc ggtcgcctgt acgctatcca tggcaccaac
    16381 gccaacttcg gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac
    16441 atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg
    16501 gtaaaagcga ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct
    16561 accaccgaag cccagtttga aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg
    16621 cagacagtga ccggtcagcc agatgttgac caggttgttc tggatgaagc gatcaaaaat
    16681 cgttccggga tgccggttcg tctgaattaa tcttcaaaac gtaaagcaaa ggcggactca
    16741 taatccgcct ttttaatttg acagacctta tcccgccggg atcataacca acaaaacata
    16801 tcccaccagc gccgcccaca acagcatcgg tatcgaataa agatgaaaac gccaccagat
    16861 acggcgatcg ttcgccatcc gtaacgcaat caaatttgcc agcgatccgg gtaataaacc
    16921 aaagccacct acgtttaccg cccatgccag taataaagac ggtggcacat aattgagcag
    16981 caaaatggta ctcggcacgt tactgatcac ctgcgataaa ccgatcgccg ttagccataa
    17041 tccgggttcg gataaatgac tcacgttact taacaccccc tgcaaaactg gcagttgggt
    17101 tagtaaatgg acgtcgataa acatcgccat aaacaccagc agcagcgtcc agtccacact
    17161 gagcaccacg cgacgcgcca gcagcgcaaa gcccgccgcc acaatcacca gtccccacag
    17221 ctcttgtttt aactccagcg ccgtcagaaa gacgatatac agccccagac aactccacac
    17281 cagccgcggt ttccactccg gcgtttgccc tcctgtatgg tactgcagcg cctttccagg
    17341 gaaacaacac cagcacaaca gcaggagtgt cagcatcatt gcgccagcca gcggtgccat
    17401 ttgggcaata aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg
    17461 gttgccaatt ggcgtcagta gcgaaccagc attgactgcc agcgcctcaa aaataatcag
    17521 ccgattaacc gggatctcac acagtctttt tagcgtgata gtcagcggaa caactataaa
    17581 cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc gccgccagta ccataaacat
    17641 cgccagccga cgctccgtag caaaacggcg caccattttg cgccccagca catcaaaata
    17701 accgcttaac tcgacacctt tagtcagcag catcaggccg cttaaggtga tgatggtgtg
    17761 ccagtcgata gcagcaggcc aggatttcgg tgcaaaggga acgaaaaaac ttaatccgat
    17821 gccaacaaga attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag
    17881 gctcatttgg caccgtgttt ctgggtaaac aaacgaaagg catccagcgt ctcttcacta
    17941 acatggtgct ccataccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc
    18001 aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct
    18061 tctgccgtta aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagt
    18121 cgcttaagca ttttagccac cgtcggttgc gaaaccccca gacgagcagc catgtctacc
    18181 tgacgagctt cccccacttc cctgatcaag tcagaaatca gctcaacata atcatcaata
    18241 agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc
    18301 actaactgcg tcactttttt tgctgttggc gtacctgcgc gacgactcat tatgcttcct
    18361 cattatggtg acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc
    18421 gaaggtacaa aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg
    18481 tatgtaatgc aatcatccct caaggatcga tgggattagc aagtcaggag gtcttatgaa
    18541 tgagttcaag aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct
    18601 gctctctatg ttgtgcgata tggtcaacaa caaaccgcag caggataaac cttccgataa
    18661 atagcggcgt cgcggtacgc cgcttcactc ctgcttttat gcaggcacaa cgcgttttgg
    18721 tctgaaaaac cccacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtcg
    18781 gtagttgacc tgaacagcag ctcgctctat cttcttgcag ccctgcgtat attgcggctc
    18841 gcggatgcgg acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca
    18901 gggtaagcac atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc
    18961 ttgttacccg tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat
    19021 taattaacct cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta
    19081 ttttgctttt gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtatcagtt
    19141 cgctggtggc ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aacttcaata
    19201 cattgctggc attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc
    19261 cgtggtacag ctacctggtc ggcatattta tttttgcgct cggcgtaata gcaatcaggc
    19321 gaaaaaaaga gagtgaaaaa gcgcgctgga ataccttcga cagcctgtgc ctggtattca
    19381 gtgtggcgac attttttgtt gctcccgtgc aaaacctggc ctggggcggc gtatttaaac
    19441 tgaaagatac tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg
    19501 aggtgattga agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg
    19561 tcactgccgt taagccgaag tatcagacct atgtggtggt gatcggagaa agcgcgcgtc
    19621 gcgatgccct cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta
    19681 acggtttgat atttgctgac tacattgccg ccagtggctc cacgcagaaa tcgcttggct
    19741 taacgctcaa tcgcgttgtc gatggcaaac cacagtttca ggataacttt gtcaccctgg
    19801 caaatcgcgc gggcttccag acctggtggt tttccaacca gggtcaaatc ggcgaatacg
    19861 ataccgccat cgccagcatc gccaaacgag cagatgaagt gtacttcctg aaagaaggta
    19921 attttgaagc agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc
    19981 tggcgcaaga gcactcgcaa ccgcagctga ttgttctaca tctgatgggc tcgcatccgc
    20041 aggcctgcga ccggacacaa ggaaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt
    20101 gctatctcta taccatgacg caaacagacg atttactgcg caagctgtac gatcagttac
    20161 gcaacagcgg cagcagcttc tcgctggttt acttttctga ccacggtctg gcctttaaag
    20221 agcgcggcaa agacgtgcaa taccttgctc atgatgataa gtatcagcaa aatttccagg
    20281 tgccttttat ggtcatttcc agcgacgata aagcgcatcg ggtgattaaa gcccgccgct
    20341 cggccaatga cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa
    20401 atatcaaata cccgtttata tctgaaaaga aagccgggcc gatatacatc accaacttcc
    20461 agttacagaa ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa
    20521 acaaaaaaat ccgccccgag aggcggattt ttatatcacc gaagtgatta gaagcggtaa
    20581 ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag
    20641 aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg
    20701 tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc
    20761 acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg
    20821 atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg
    20881 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
    20941 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
    21001 acagtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct
    21061 gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt
    21121 taaatatatc aaaaattaat gggaaactct tcgcgattta tgatgtctaa cgggccattt
    21181 catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag
    21241 aactgcgtaa gtattacgca tattttccct gtcattcatc cagattattc ctaatcacca
    21301 gactaatgat tccatca
//
LOCUS       DACADA010000072        21240 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.073, whole genome shotgun sequence.
ACCESSION   DACADA010000072 DACADA010000000
VERSION     DACADA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.073"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..329
                     /locus_tag="ISB17_002639"
     CDS             <1..329
                     /locus_tag="ISB17_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1980555.1"
                     /translation="KITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKW
                     SLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQ
                     PVSLAG"
     gene            complement(372..1523)
                     /locus_tag="ISB17_002640"
     CDS             complement(372..1523)
                     /locus_tag="ISB17_002640"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1980556.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1537..3132)
                     /locus_tag="ISB17_002641"
     CDS             complement(1537..3132)
                     /locus_tag="ISB17_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAP1980557.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3279..4037)
                     /gene="aroD"
                     /locus_tag="ISB17_002642"
     CDS             complement(3279..4037)
                     /gene="aroD"
                     /locus_tag="ISB17_002642"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP1980558.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4068..4934)
                     /gene="ydiB"
                     /locus_tag="ISB17_002643"
     CDS             complement(4068..4934)
                     /gene="ydiB"
                     /locus_tag="ISB17_002643"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAP1980559.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMEPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4946..6211)
                     /locus_tag="ISB17_002644"
     CDS             complement(4946..6211)
                     /locus_tag="ISB17_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980560.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6438..7652)
                     /locus_tag="ISB17_002645"
     CDS             complement(6438..7652)
                     /locus_tag="ISB17_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980561.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7752..8108)
                     /locus_tag="ISB17_002646"
     CDS             complement(7752..8108)
                     /locus_tag="ISB17_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAP1980562.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8244..8351)
                     /gene="rprA"
                     /locus_tag="ISB17_002647"
     ncRNA           complement(8244..8351)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ISB17_002647"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8537..9649)
                     /gene="ydiK"
                     /locus_tag="ISB17_002648"
     CDS             complement(8537..9649)
                     /gene="ydiK"
                     /locus_tag="ISB17_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAP1980563.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9863..12919
                     /locus_tag="ISB17_002649"
     CDS             9863..12919
                     /locus_tag="ISB17_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP1980564.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12916..13326
                     /gene="menI"
                     /locus_tag="ISB17_002650"
     CDS             12916..13326
                     /gene="menI"
                     /locus_tag="ISB17_002650"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAP1980565.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSVREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13426..13614
                     /locus_tag="ISB17_002651"
     CDS             13426..13614
                     /locus_tag="ISB17_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980566.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14163..14531
                     /gene="sufA"
                     /locus_tag="ISB17_002652"
     CDS             14163..14531
                     /gene="sufA"
                     /locus_tag="ISB17_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAP1980567.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14540..16027
                     /gene="sufB"
                     /locus_tag="ISB17_002653"
     CDS             14540..16027
                     /gene="sufB"
                     /locus_tag="ISB17_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP1980568.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTVISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16037..16783
                     /gene="sufC"
                     /locus_tag="ISB17_002654"
     CDS             16037..16783
                     /gene="sufC"
                     /locus_tag="ISB17_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP1980569.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16758..18029
                     /gene="sufD"
                     /locus_tag="ISB17_002655"
     CDS             16758..18029
                     /gene="sufD"
                     /locus_tag="ISB17_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP1980570.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPTKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVMARIGQRLPGGAR"
     gene            18026..19246
                     /gene="sufS"
                     /locus_tag="ISB17_002656"
     CDS             18026..19246
                     /gene="sufS"
                     /locus_tag="ISB17_002656"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAP1980571.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGVLYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALAQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19259..19675
                     /gene="sufE"
                     /locus_tag="ISB17_002657"
     CDS             19259..19675
                     /gene="sufE"
                     /locus_tag="ISB17_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAP1980572.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19823..20827
                     /gene="ldtE"
                     /locus_tag="ISB17_002658"
     CDS             19823..20827
                     /gene="ldtE"
                     /locus_tag="ISB17_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAP1980573.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20890..21126)
                     /locus_tag="ISB17_002659"
     CDS             complement(20890..21126)
                     /locus_tag="ISB17_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAP1980574.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5513 a         5149 c         5381 g         5197 t
ORIGIN      
        1 aaaaaatcac tttagagcag ttatcgaaaa aatttcgtgc cagcgttagt tatatctgcc
       61 atgaatttac caaagagtat cgtatttccc ctattaacta tgttattcaa cggcgtatga
      121 cggaagcgaa atggtcactc actaatactg aattatcaca ggcagagatc tcctggcgtg
      181 tgggttatga aaatgtcgat cactttgcca aactgttttt gcgccatgtc ggctgttcgc
      241 ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga acaagaaatc ctatctgaat
      301 ttcctcaacc agtaagtctt gccggataaa tctggcggcg cagtttgtta agaagctgcg
      361 ccgcctgcag attatttgtt ctgataatct ttcaggatct gccgacctgc tacgtaaatc
      421 ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga catcacgcca gaagcgggag
      481 acgcgcgcct catcggtata gcccagaccg cccatgattt gaatcgcatc atcaatgact
      541 tccattgcgg tacgtgcgca gtacagtttt gccagcgccg cgctggtgcg cagtgactga
      601 tgctgatcgg cttgccatgc cactttcaac accatgttgc gcatgttgtc gatcttaatc
      661 gccatcagcg ccagtttttc ctggatcatc tggttatgac caatgggctt accaaaagcg
      721 atacgctggt tggcatagcg ggcggcatct tcgaatgcgc attcggcaaa gccggtgctg
      781 cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca tcacattgag gaaacccatt
      841 ccttcttcgc ccaccatgtc gctctcttca acttccacgt tgtcgagata gacttcgcag
      901 gtgctgagca tatgccagcc gattttatgc agtggattga ttttaatgcc gggcttactg
      961 gagtcgaccc accacagagt aaaggccttt ttcggatctt tcggttgcgg atcgcgcgcc
     1021 aacaccagca tatatggata ctctttcgca ccggtaataa aggttttctg tccgttgatg
     1081 taaaccttgc cgtttttacg cgtgtaagtg gtagtggcac tgttgttgtc tgagcctgcg
     1141 cctggctccg tcaatgccag ggcataggcg ggatcacccg tttccagggt gctctctgcc
     1201 gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg
     1261 gtaatcaaaa atgccggagc accgcatttt gacacttcca tcagcgccag catttgggtg
     1321 acgtaatccg cagggatacc accaaattct tccggcacgc caagcatgga aataccgtta
     1381 tccgccagtg cccgcataaa ctcacgcggg tatgtcccgt tttgatcgca ggtgcggaaa
     1441 tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag cagttcttgt
     1501 tcttcagtta aagagaaatc catcattata ctcctgttaa tgagccgctt caggcaggac
     1561 aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt ctggcgaaat
     1621 cactggggtg aaatccattt tgtcgagaat atctttttgt aaatcgacgc caggagcgat
     1681 ttcgattaaa tgcaggccgt cttcttttag cgtgaatacg gcgcgctcag tgatataacg
     1741 aacatccagc cctcgctcga gagcgatttt tccgctgaaa gttatttccg gtagttcccg
     1801 aataaatttc ttcacccgtc cttcctggac gatatttaat ttgccgtcgg taatttctgt
     1861 ttttaaactg cccgcagtta atgtgccgca gaaaacgatt ttcttcgaag tggcactgat
     1921 atcaataaat ccaccggtgc ccatgatttt accattgaat ttatgcacgc cgacgttacc
     1981 gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccaccac cgtgataaaa
     2041 atcaaactgg gacgtcatat ccagaatggc gcgggtattc acgttcgcgc caaaggcgat
     2101 cccctgtgaa gtaataccgc caatcggacc tgtttctacc gtcagaataa agtcatcagc
     2161 acaaccttct tcccgggcga ccaggccaat gccgtcagca ataccgacgc cgacattccc
     2221 cactgcgcct ttgcgcattt cgaataatgc acgccgtgca actaatttac gttggtttag
     2281 gggcaggcta agtttggtgc tgtcatcaag ggtgaagtca ccagaaataa agcggttaac
     2341 cggcgcaccg ccatacagtt gggtttgatc cggatcgacc accacaatat ccaccagata
     2401 acccggaata cggacagatt taggatgcag cgtggctttc ttaaccattt tctgcacctg
     2461 catcatcaca ataccgccgt tattgtgcac cgcctgggca ataaccaatg cgtcgagata
     2521 catcacctca tcttcaaaag tggcgtagcc ttcgctgtcg caggtggtag cgcgaatgaa
     2581 ggcgatatct ggcgcaatcg ctttgtaata gagatattct ttgttatcaa actcgaccag
     2641 tttaatcagg tcttctttag tgacttcatt cagtttgccg ccttgctggc gtggatcgac
     2701 aaatgttccg atgccaatat cactaataat accaggctgg tgggctgcgg cggcgcgtaa
     2761 ggtttgtgta agtacacctt gtggatagtt ataagcaata attttatttt gttctgcgag
     2821 atcagaaata cgcggtgatt gtccccagtg accgcataat gcccatttca ccaggccttc
     2881 ttgcgccagc ggactaatac cacggtcggc gcgatcgcca agccctgttg gactaataat
     2941 cgataaatta cgtggtgttt gagtctgttt atatttatca gcaagagcag taattaacgt
     3001 ggtggcttcc agaataccgc cgccagcgcc taacacacaa agtgttgctt cgtcgggaat
     3061 ataattcacc gcttcctgtg ccgataggac cggcactcgt ccattaatac gaggtggttt
     3121 tacaggtttc attgttaatt cctgattcag gtgtgatttt ccctacgcca gatggagaat
     3181 tagcgcacag agactcacga aatattttta ttaaatacgg ttaaaaatac tttttttagt
     3241 tcggcgggag agggtattcc cgccgaaaat attattgctt atgcctgatg taaaatagtt
     3301 aataccgtac gcaaatcatt taccgagatt tgccctggcg cagaggcttt ttttaccgca
     3361 ccaaaagttg ccgccgaccc aaatacttca ccagccagac gagaaattac gccagttttt
     3421 gccatcgaca tcgtgatgat tggacgatcg gcatactgct cctgcatctc cagggtcgcg
     3481 gcaagcaacg tcagcacatc gctggtactt tgcggcatca gcgcaatctt aggaatatcg
     3541 gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt cttcggcttc cggcgtttta
     3601 tggaagtcat ggttggacat gacaactttc acatcatgcg cgtgggcgta ggcgacggtt
     3661 tctttaacct gatcatcgcc ggtaaataac tccagatcga tcatatcaac caggccgctg
     3721 tcgatggctg cacgattgag tgcaatataa gcctcggtgg aaatcgcctg ctcgccgcct
     3781 tctttggcac tgcggaaggt aaacagcagc ggtttttctg gcatggtttc acggagaatt
     3841 tttgctgccg ccatgacaga ctccacattg gagaggtcgg catagtggtc cacacgccat
     3901 tccagaatat caaagtccgc ttcacgatag gcgagagctt cggatttcac gctggcgata
     3961 tctttcgcca tcagcgagac gatgatttta ggtgcgcccg taccaatgac gagatctttt
     4021 acagttacgg ttttcatttt ttaccctttc tgcacgcggt cagcctgtca ggcaccgaac
     4081 cccatgacct gtttaacata ttccagaggg aaatctttgc cagtccataa cgtgaactgt
     4141 tcagcccctt gccacaacaa catgccgtat ccatcaatcg ttttgcaacc cgcttgttgc
     4201 gcctgctgca ataacttcgt catatgaggg ttatacacgc attcagtgac cagaagtccc
     4261 gggtgtaaca gactgatatc attaaccaat gattcattct caaggggttc catacccact
     4321 ttggtgccat tggttaaaat gtcggcggaa gccagggctt ctgcaaaggc ttgctgatcg
     4381 gcgagatcgg tgactgtcac gacgcaatcg gtgttttcgt taacccgctg cgcgaaggcg
     4441 agagctttat cgaagaactc atcccgacgg ttaaagagtt taatttcttt taaaccttca
     4501 attgccccct gcgcgccaat tgccgttgag gcaccaccgg cccctaatag caccatcgtt
     4561 ttgcctttga tatcaaaacc gctctcttta atggcgcgaa tatgacccgt gccgtcggtg
     4621 ttatagccac gcagatagcc atcatcattt acgatggtgt tgatggcacc caccagtttg
     4681 gccgctggcg ttaattcatc aacatattca cacgccagtt gtttgttcgg cattgataca
     4741 ccagttccgc gcattttgag ggcttttaat ccttcaattg ctccgggaaa actatcgtta
     4801 tccacttcaa aggccatata ggtaaatggc aatcccgctt tttctaaggc tttattctgc
     4861 atttcgggcg ataaactgtg gcggatggga taggccatca acccaatcaa ttcgtatttt
     4921 gcggtaacat ccataattaa ctcctttaac ctctatgctt aattgtgttt aaccgggtgc
     4981 taaatttatg ctcgccaaac cgcacatcat tttcaggaat aatgaaaacg cggtaatagc
     5041 ggataaaaac aataattgcg gtaatcaggg ccagcagcgc gaaagtaaaa tcgagaacaa
     5101 tgatatattg caggccgatg ttcgacagat aaccggtaat cagcggaata acaaagttag
     5161 ccagtccacc catcatcata tatatactgg tgactttggc tttgcttttt gggaaaaact
     5221 ctgacatgac cgaaacgccg agctgtaaaa tgccgccagc tgctgagaaa ccgataacaa
     5281 aggctccggc attacacacc aatggagatg ggtacaggta gataatggca gctgttattg
     5341 ttgccagtgc agagttaaat acattagccc agatgggccg gaccattttt ttcagcagtg
     5401 cggcaaaaat aaagacacag accagcgaac ccatactgta ataagagatg gtttttaatg
     5461 cctcagcttc tgacatacca gcaaaagcca tcgcatattt gggcatccac accacaatca
     5521 cataaaaggt cgagaatgcg gctacaccga acagtactga tgaaacacct tccagccaga
     5581 ctaacggttt gctgttcatt tgcggtaatt cattagctac gctggcgtcc accaactggc
     5641 tggggaattt gcttttcaac agcatcagcg tgatcagtac aaacagaata cccggaataa
     5701 tcagcccata gccgtaccag atgttattga gcaacatata gctcaccagc attggataga
     5761 acatttgccc gaatgacacc atcgctttaa ccagtatgac cgccgaacca gaggctttcg
     5821 gaaagcattc catgagcgcg gggtagccac ccgtatccag cgctgagtta gcgataccta
     5881 cgcacactgc cagacagtag gcgagagtta aattcgggca agcaggaata ccaaagaaga
     5941 atagcagata cattattact gccattaata tcaccgcccg acgaccaaac ttatcggaga
     6001 tcactccgaa gaataaaata ctgatcaatc gccccaatcc gataccggaa attaaatagg
     6061 caatgcccgc gttgtcagtg gaaaactttt ctgccaaaga tgtcatgttt tgggcaagcg
     6121 taataacact aatgccgtgc aggaaatagc tgaagtaaat acaaagaaca gccaggataa
     6181 atggcgtgct gaaagcctta ttttgagaca tgttcacagc tccttgctga aagataattt
     6241 cgttaccgta cgtattgttc tccgacggtt tacataatta ctatactgaa gatgattttc
     6301 cctgggtaca tggcataaag tttatgtttc caggaatatt gcatgtcata gtttgttttt
     6361 gatcgctatt ttccttagtt agcgcagaaa gagttattta tccgtaagat cttgcataac
     6421 ctgaaaagaa tattgcatta cccacccgga gcgatatttt cctttagcgt gtgatgccgc
     6481 gtttctttgc gactgcgtaa gccaataaac agtgccagta taaagccgat agcagcgatg
     6541 gcggtatcga accacataat atcggcaata cttctttgcg aaagatgagc cgtaatcaac
     6601 ggaatggtaa atgttgcaat gctgcccgca ctgtaataga tccccgtagc tttaccttta
     6661 gcgtagggaa aacgttccgc cattaacgtc aggccaattt gtacaacacc gcctgcggag
     6721 gtaaaaccga tgacgaaagc aaatattatc accacataaa atgtgggatg caggcagacg
     6781 gtgagcaggg cgataaatga gataaaggtg tacagcatca gtaatgttgt tgggcgaacg
     6841 gtattacgaa tgagtggagc agtaataaat acacacagca gcgaacccac ggtgtagata
     6901 ctgagtaatt tgatcgacat ggtgtatgac atgccagcga caaattgtcc gtattgtgcc
     6961 agccactggc taaccagata aaacgttgcc attgagatat agccatatag ggaataactg
     7021 gctaaatcga taattgaaca gcgatgttcc gtagagctgg tggttttctt tatgacaggt
     7081 aagtgacgac ccggatgggg tgggaacgta caacggtata aaaacagagc gttaataaac
     7141 ataatgcctg cggcaatcat aaaggaccaa ccgaaccaca gttcagccca cactaacagg
     7201 ctgataatta gcggtaataa aaattgtccg ctggaaacaa atgctttaat taaaatattg
     7261 gctgtacccg gtgagcgtgg aaaagcttcc atcaaactgg gataggtgcc tgcatcgaga
     7321 aaactgtttg ccattcccgc cagaaagcca aaaacataag cgataatgat gttattggtg
     7381 tgcaggatgc caaaaaagaa ggccatatag cagcacatcc cgagcatgat aaaagggcgg
     7441 cgaccaaagc gatcggataa taatcctgca aaaagcaaga cacttaatcg accaatgccc
     7501 agtgatgaga taactatcga gacgcccgcg gcattagtct gccaaagcgt ctccagcgag
     7561 gccatattca ggctcatcaa aatgacgccc ataccatgca ccaggtaatt aaaatacaac
     7621 ccaagtgcgg tagggtaata gggatttttc ataggttcta cttagcaaag gtgctttaaa
     7681 aagtgtagcg atgttgtgtc agtatccgtt cattattgtt gtaacaacga caattccttt
     7741 ctgtggagaa attaacaaag ccgctccaga tcgtgtgcat ataattcagc ggctaccgat
     7801 tgatagatct tccagtcaat aaaattgcca tcagggtaga cttgttgaaa cgcggttaag
     7861 tcggggaaaa aacgcatggt gttgttgccg atgcggttat ttattttctc tatgattgca
     7921 tgaagatgtt tttttcgttg ctgacgcata gccaacagtt gctcgactac ataatcagga
     7981 atggcgcatt tgccattttc ccactgacgc cagctttcac tgttaccggt ctgggcaatc
     8041 caggtcgcgc attcatcaat agtcatggca aaaatatggc gaagtgcctg gagttcataa
     8101 gcgttcatcg tgtaatggct gcatgaaata aacaataaaa tataaataac attatcagcc
     8161 tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg
     8221 cgaggtagcg aagcagaaaa atgttaaaaa aaagcccatc gtgggagatg ggcaaagact
     8281 acacacagca attcgttgtt tcactcaggg gatttccatg cttataaatc aatatgttga
     8341 tttataaccg tgagctaata gtaggcatac ggactttttg cgtcgatcag attcgtctca
     8401 ttagtttaaa aactgtaaag aatttgcgac aggtactacc agctgaatag cggtaagcag
     8461 gaaaatatgg ggaagagcaa gcaaatatca attagcaggg gttaacaatc aggcggcaga
     8521 gccgcctgat gagaaattac ttattcgatt tctcgatttc gccaagttct tcgagaattt
     8581 cttccggttg gtccgtcggc ggtgggactt cttccaccca cgcggcaaac agacgccagg
     8641 aaacggctaa cagaaccgga ccaataaaca gaccgatcat cccgaaagca atcaaaccac
     8701 caataacgcc agagaggatc aggatcagcg gtaaatcggc acccatgcga attaacattg
     8761 ggcggatgac gttatcgagc gtgccaacca caccgctcca aactaacaat accgttcccc
     8821 aggtggtatc gccagtccag tagagccaga taatcgccgg aatcagtacc ggcaacggtc
     8881 caagctggac aaggcaggag aggatcatta acaccgttag caaagttgca taaggtacgc
     8941 cggatacggc gaggccgata ccgccaagca ctgcctgtac taacgccgtc accaccacac
     9001 ccagcgccac cgcgcggata gcctgtgccg ccagcagcac ggcggcatct ccgcgaacgc
     9061 ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac ctgttcaccg cgccagtaca
     9121 gcagggcgct gaagagaagc atcagcgcac aatgcaccat aaagcgcccg atatgcgccg
     9181 cctgcccaac gaaccaggtg gtggtggtgc caatataagg acggactttc gccatgatcg
     9241 ccgtcccccc catatccagc aaattgtgcc agcctgcata tagcttcgcg ccaatcaccg
     9301 gaatggtatt aagccacgcc agatcgggta acgtcatgtc accactggaa atggctttaa
     9361 ttagcgggcc gctgccgtcg acgatactgt taaccagcaa agcaataggg atgataaaca
     9421 ccatcactaa taacagcgtc atcaccagaa cggcgagaga gcggcgacca aacatgatct
     9481 tttgcaaacg taacaatacc ggccaggtgg cgataaccac tgtaccggcc catgcaaagc
     9541 cgagaataaa gggttgtaca atccacagac atgccacaat catgatggct aaaaacagca
     9601 ccgaaagcag aatttgtgcg acatccctgg gctgacgaac atttaccata actacttttc
     9661 accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac gcgaaactca cctgcataat
     9721 gatgagcaat ttcagcggtt tttaacaggt cgattctgcc cttaattctg atgggcgtgt
     9781 tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa cgattaatct ctcatcacag
     9841 accgcaggaa agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca
     9901 actggtgctt aattttttgc aagagctgga gcaacagggt tttaccggcg atacggcgac
     9961 aagttatgcc gatcgtctga caatgtcgac tgacaacagt atttaccaac ttctccccga
    10021 tgcggtggta tttccgcgtt caaccgcaga tgtagcgctg atcgcccgtc ttgccgcgca
    10081 ggaacgctat tcatcgttga tctttacccc ccgcggcggc ggcaccggca ctaacggtca
    10141 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
    10201 taaccctgaa gagggctggg tgcgcgttga ggccggggtg attaaagacc aactcaatca
    10261 gtacctgaaa ccctttggct acttttttgc gccagaactt tcgaccagca accgggcaac
    10321 gctcggcggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
    10381 gtcagatcac gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
    10441 acctttaccc gtcgaactgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
    10501 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc
    10561 caagcttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac
    10621 cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
    10681 agcgcgactg gatattacgc gcttgcctaa agtacgccgt ctggtgaacg tcaaatatga
    10741 ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt gaggcgcgcg cgctttcggt
    10801 agagacggtg gactcaaaag tgctcaacct tgcacgggaa gatattgtct ggcattccgt
    10861 cagcgagttg attaccgatg tgcctgataa agagatgctt gggctgaaca ttgtggaatt
    10921 tgccggtgat gatgaggcac tgattgacga gcgagttaat gcactctgtg tgcggcttga
    10981 tgagctgatt gccagccaac aggcaggagt gatcggctgg caggtgtgcc gcgagctggc
    11041 gggtgttgaa cgtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa
    11101 aggtgctgct aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacacctggc
    11161 ggattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
    11221 cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggcg ctggatatgt gcgatccgca
    11281 acaagagatt ttgatgaagc aaatctctga tgatgtagtg gcgcttaccg cgaaatacgg
    11341 tggtttgttg tggggcgaac acggcaaagg ttttcgcgcc gaatacagcc cggcgttttt
    11401 cggtgaggaa ctttttgcag aactgcgtaa agtgaaagcg gcatttgacc cgcataaccg
    11461 actcaacccc gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga
    11521 cgcggtgaag cgcggtacat tcgatcgaca gatccccatt gcggtacgcc agcagtggcg
    11581 tggtgcaatg gagtgtaacg gcaacggttt atgcttcaac tttgatgctc gtagtccgat
    11641 gtgtccgtcg atgaagatca cccagaaccg gattcattca ccgaaagggc gcgctacgct
    11701 ggtacgtgaa tggctgcgtt tgttggcgga tcgcggcgtt gacccactca aactggaaca
    11761 agaacttcct gaatccggag tcagtttgcg gacgttaatt gcccgcacgc gcaatagttg
    11821 gcatgcgaat aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg
    11881 tctggcctgt aaagcgtgtt cgactcagtg ccccatcaaa attgatgtgc cggagtttcg
    11941 ctctcgtttc ctgcaactct atcacacccg ttatttacgc ccgctgcgcg accacctcgt
    12001 cgctacggtt gagagttacg caccgctgat ggcacgcgcg ccgaaaacct ttaatttctt
    12061 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
    12121 gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
    12181 gctggaacag cttgaagctc tcaatgcaga gcagaaagcg cgcacggtgc ttgtggtgca
    12241 ggaccccttt accagctatt acgatgctca agtggtggcg gattttgtcc gtctggtcga
    12301 aaaattaggt ttccagcccg tgttactgcc attttcgcca aatggcaaag cccagcatat
    12361 caaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
    12421 tatggcgaag ctgggtatgc caatggtagg cgtcgacccg gcgctggtac tttgttatcg
    12481 cgatgaatat aaactggctc tgggcgagga gcgtggcgag tttaacgtct tactggcgaa
    12541 tgaatggctg gcaagcgcac ttgagtcaca gccagtggct acagtcagcg gtgaatcatg
    12601 gtatttcttt ggtcactgta ccgaagttac cgccttgccg ggcgcgccag cacaatgggc
    12661 ggcaatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtggggt gctgcggcat
    12721 ggcagggact tacggacatg aagcgaaaaa tcataaaaat tcgctcggga tctatgagtt
    12781 atcctggcat caggcgatgc agcgactgcc gcgtaatcgc tgtctggcga ccggatattc
    12841 ctgccgtagt caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt
    12901 actggagatt attaaatgat atggaaacga aaaatcaccc tggaagcact gaatgctatg
    12961 ggggaaggaa acatggtggg gttgctggat attcgctttg aacatattgg tgatgacacc
    13021 cttgaagcga caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga
    13081 ggtgcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgag
    13141 ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc acgtccgctc agtacgagaa
    13201 gggcgggtgc gcggcgtatg taaaccgttg catctcggtt cgcgtcacca ggtctggcag
    13261 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgctatt
    13321 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggttatta tctgcacagt
    13381 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
    13441 gatcccaccc aactggccat tgaattttta cgccgtgacc aaagcaatct ttcacctgcc
    13501 caatatctca agcgtctgaa acaattagaa cttgaatttg ctgacctgct tactctctct
    13561 tctgctgaat taaaagaaga aatctatttc gcctggcgtc tgggcgtgca ttgatcctga
    13621 tttaatgtcg cgagttcgcc aaatcggcct ggattaatct tcgtccaggt tcgagggcct
    13681 tcttaatact gcctatctac ccgcctgata acgttcaaaa ataatacaaa tcgcagtttg
    13741 tgttaaaaca gtgggttagc tttatgagat tatccttatc gccccttcaa gagctaagcc
    13801 actgtgagtg ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca
    13861 gacttaaggg ttttcttatt tctgaacgct gcactttttt acctatccct taaatagtca
    13921 ttttcaatgc aactttttct ttttcatcac agccttaatg ctccttagat aataatcatt
    13981 atctaacaat gagataccta attcttagcg tgcctgttaa cccacaaatc agggtctatg
    14041 cttattaaaa gcaggtaaaa agcatgtttt acgctaaagc ccctgcgttt gctgggttga
    14101 actgataatc attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat
    14161 cgatggacat gcattcagga acctttaatc cacaggattt cgcctggcaa ggcttaacgc
    14221 tgacacccgc agcggcgata cacatccgcg aactggtggc aaagcagccg ggtatgatcg
    14281 gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt tggctatgtg ctcgacagtg
    14341 ttagcgagcc ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc
    14401 cgctgcaagc gatgcctttt attgatggca cggaagtcga tttcgttcgt gaaggactta
    14461 atcagatatt caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct
    14521 ttggggtata ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg
    14581 gaccggcggc ccgctgaatt ataaagaagg attcttcacc cagttagcca ccgatgagct
    14641 ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga
    14701 gtggatgctg gagtttcgtc tcaacgccta tcacgcatgg ctggagatgg aagaaccgca
    14761 ctggttgaaa gcgcactacg acaagctgaa ttatcaggat tacagctact actcggcacc
    14821 atcgtgcggt aattgtgacg acaactgcgc gtctgaaccc ggcgcggtgc agcaaactgg
    14881 cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt
    14941 gcgggaaggc aaagaggtgg cggtggatgc cattttcgac tctgtttcgg ttgccactac
    15001 ttatcgcgaa aaactggcgg agcagggaat tattttttgt tcctttggcg aggcgatcca
    15061 cgatcacccg gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt
    15121 ctttgccgca cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg
    15181 cgtgcgttgc ccgatggaac tttccaccta ttttcgcatt aacgcggaaa aaaccgggca
    15241 gtttgagcgc accattctgg tggccgacga agacagctac gttagctaca ttgaaggctg
    15301 ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg gtggtggaag tcatcatcca
    15361 taaaaacgcc gaggtgaaat attctacggt acaaaactgg ttccctggcg ataacaacac
    15421 cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat
    15481 gtcatggacg caatcagaaa ccgggtcagc gattacgtgg aaatatccca gctgcatttt
    15541 gcgcggcgat aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcaaca
    15601 agcggatacc ggcaccaaga tgatccacat cggtaaaaac accaaatcga ccgttatctc
    15661 gaaagggatc tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc
    15721 gacggcaacc aatgcgcgca atttcactca gtgcgactca atgctgattg gcgctaattg
    15781 tggggcgcat accttcccgt atgtcgaatg tcgcaataac agcgctcagc tggagcacga
    15841 ggcaacgaca tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag
    15901 cgaagaagat gccatctcga tgattgttaa cggtttttgc aaagacgtgt tctcggaact
    15961 gccgctggaa tttgccgttg aagcacaaaa actcctcgcc atcagccttg agcatagcgt
    16021 cggataagga ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc
    16081 tatcctgcgt ggattaagcc tcgatgttcg tcccggcgaa gttcacgcca ttatggggcc
    16141 aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc gggcgagaag attatgaagt
    16201 gacgagcggc acggttgagt ttaaaggcaa agatttgctt gcgctgtcgc cggaagatcg
    16261 tgcaggtgaa ggcatcttta tggccttcca gtatccggtg gaaattcccg gcgtcagtaa
    16321 ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc tatcgcggcc aggaaacgct
    16381 cgaccgcttt gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga
    16441 agatttatta acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga
    16501 tattttgcaa atggcggtgc tggaaccgga gttatgcatt cttgatgagt cagactccgg
    16561 gctggatatt gacgcattaa aagtggttgc cgatggcgtg aactcgctgc gtgacggcaa
    16621 gcgttcattc atcattgtta cgcactatca acgcattctc gactacatca agcctgatta
    16681 cgtccatgtg ctgtatcagg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca
    16741 actggaggag cagggttatg gctggcttac cgaacagcag taacgcactg caacagtggc
    16801 atcatttgtt tgaagctgaa ggggcgaaac gctccccgca agcacagcag catttacaac
    16861 aattgctgcg taccggactg ccgacacgta aacatgaaaa ctggaaatat acgccgctgg
    16921 acgggctgac caatagccag tttgtctgca ttgcggggga gatatcccca cagcagcgtg
    16981 atgccttagc gttaacgtta gacgccgtgc ggctggtgtt tgtcgatgga cgttacgtgc
    17041 ccgcactgag cgatgcaact gaaggcagcg gatatgaagt gagcattaac gacgaccgtc
    17101 aggggttacc cgacgctatt caggcggaag tgtttctgca tttgacggaa agcctggcac
    17161 aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc gacaaagcca ttgctgttaa
    17221 tgcatatcac ccagggcgtg gcaggtgaag aggtgaatac tgcccattac cggcatcatc
    17281 tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg
    17341 ctcgtcactt caccggcgca cggttcacta tcaacgtcgc agcgaacgcc catttgcagc
    17401 atatcaagct ggcgtttgaa aacccgctca gtcaccactt tgctcataac gatttgttgc
    17461 tggctgatga tgccaccgca tttagccaca gtttcctgct gggtggcgca gtgttacgac
    17521 acaacaccag tacgcaactc aatggcgaaa acagcacggt gcggatcaat agcctggcga
    17581 tgccggtgaa aaacgaggtg tgtgatactc gtacctggct ggaacacaat aaaggttttt
    17641 gtaacagccg acagttgcac aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg
    17701 gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc
    17761 tgctgatggg caaactggcg gaagtggata ctaaaccgca gctggaaatc tatgcagatg
    17821 atgtgaaatg cagccacggc gcgacggtgg ggcgtattga tgacgaacag atgttttatc
    17881 tgcgctcgcg cgggatcaat cagcaagatg cccagcagat gatcatctac gcctttgctg
    17941 ccgaactgac ggaagcactg tgtgatgagg ggcttaaaca gcaggtgatg gcccgaatcg
    18001 gtcaacgact gccaggaggt gcaagatgac tttttccgtc gacaaagtgc gggccgactt
    18061 tccggtgctt tctcgtgagg taaacggttt gccgctggct tatctcgaca gtgccgccag
    18121 tgcgcagaaa ccaagccagg tgattgacgc cgaggctgag ttttatcgtc acggctacgc
    18181 ggcggtgcat cgcggtattc ataccttaag cgcccaggcg accgagaaaa tggagaacgt
    18241 gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg
    18301 cggcacgacg gaagggatca atctggtcgc caatagctgg ggcaacagca atgtgcgagc
    18361 gggcgataac atcatcatca gtcagatgga gcaccacgct aacattgtcc cctggcagat
    18421 gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg ctcaaccccg acggtactct
    18481 gcaactggag acgctgccta cgctgtttga tgagaaaact cgcctgctgg caattactca
    18541 tgtctccaac gtgcttggga cagaaaatcc gctggcggag atgatcacac ttgcgcatca
    18601 acatggcgca aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt
    18661 tcaggcgctg gattgcgatt tctacgtgtt ttccgggcat aaactgtatg gtcccaccgg
    18721 cattggcgtg ctgtatgtca aagaagcctt gttgcaggag atgccgccgt gggaaggggg
    18781 cggttctatg attgccaccg ttagcctgag tgaaggcact acctggacca aagcgccgtg
    18841 gcggtttgaa gccggtacgc ccaataccgg aggtattatt ggccttggcg cggcgctgga
    18901 gtatgtttcg gcgctggggc ttaataacat agccgagtat gaacagaatc tgatgcacta
    18961 cgcgctagca cagctggaat ctgtaccgga tctcactctc tatggcccac agaacaggct
    19021 tggcgttatt gcttttaatc tcggtaaaca ccacgcctat gatgttggca gttttctcga
    19081 taattacggc attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta
    19141 caacgtcccc gcgatgtgtc gggcgtcgct ggcaatgtac aacacccatg aagaagtgga
    19201 tcgtctggta actggattgc aacgtattca tcgtctactg gggtaaaagg gaggctctat
    19261 ggctgtattg ccggataaag aaaaattgct gcgtaacttt ttacgctgcg ccaactggga
    19321 agagaaatat ctctacatta ttgaactggg acagcgtctg ccagaattac gtgacgaaga
    19381 caaaagcccg caaaatagca tccagggctg ccagagtcag gtgtggattg taatgcgcca
    19441 gaatgcgcag ggaattattg aattgcaggg cgacagcgat gcggccattg taaaagggct
    19501 tattgcggtc gtctttattc tttacgatca aatgacgccg caggatattg tcaatttcga
    19561 tgtgcgcccg tggtttgaaa aaatggcgct cacccaacat ctcaccccat cacgctcaca
    19621 aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa
    19681 agggacagct ttcatccggt tggctcacat tgtcgggtgg tcaatgttta cactggcctg
    19741 cacaggagtg ttttggtttc aggtgaacta agcgccatcc gacgtttcag cgtgagtctc
    19801 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    19861 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggcag
    19921 ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc
    19981 catcgcccga cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc
    20041 cccggtgcca aaacccggta cgacgataac tattccttca caactgttat tacctgatgc
    20101 accgcgccag gggataattg tgaaccttgc agaactgcgc ctttattatt atccgccggg
    20161 agaaaatatc gtgcaggttt atccgatagg tattggtttg caggggctgg aaacgccggt
    20221 gatggaaacg cgtgtggggc agaaaatccc taacccaacc tggacgccta cggcgggcat
    20281 tcgtcaacgt tcgctggagc gtggcattaa attaccgccg gtcgttcctg ccgggccaaa
    20341 taacccgcta ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca
    20401 tggcaccagt gcgccggaca gcgtcggttt gcgcgtcagt tcgggctgta ttcgcatgaa
    20461 tgcaccggat attaaagctt tattctccag cgtgcggacg ggaacgccgg tgaaagtgat
    20521 caacgaaccg gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg
    20581 accactatcg gcagaagaac agcagaacgt tcagacaatg ccgtacacgc tgccagcagg
    20641 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    20701 tcgtcgggca gggtatccgg ttgcggtgag cagtggagca actcccgcag ccagcaatgc
    20761 gccttcagta gagtcagcgc agaatgggga accagagcaa gggaatatgt tacgcgcgac
    20821 gcagtagcgg taaatagcag gcaaaaaaat ggcgcacaat gtgcgccatt tttcacttca
    20881 caggtactat tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc
    20941 tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg
    21001 gtcaacttta gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct
    21061 ggagcaacct gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc
    21121 tttcattatt aataccctct agattgagtt aatctccatg tagcgttaca agtattacac
    21181 aaagtttttt atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc
//
LOCUS       DACADA010000073        21061 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.074, whole genome shotgun sequence.
ACCESSION   DACADA010000073 DACADA010000000
VERSION     DACADA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.074"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            133..1155
                     /locus_tag="ISB17_002660"
     CDS             133..1155
                     /locus_tag="ISB17_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAP1980575.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQHITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1155..2135
                     /locus_tag="ISB17_002661"
     CDS             1155..2135
                     /locus_tag="ISB17_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1980576.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            2132..2890
                     /locus_tag="ISB17_002662"
     CDS             2132..2890
                     /locus_tag="ISB17_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1980577.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2900..3712
                     /locus_tag="ISB17_002663"
     CDS             2900..3712
                     /locus_tag="ISB17_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP1980578.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3709..4563
                     /locus_tag="ISB17_002664"
     CDS             3709..4563
                     /locus_tag="ISB17_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAP1980579.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4589..6559
                     /locus_tag="ISB17_002665"
     CDS             4589..6559
                     /locus_tag="ISB17_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1980580.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6609..6863)
                     /locus_tag="ISB17_002666"
     CDS             complement(6609..6863)
                     /locus_tag="ISB17_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP1980581.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7064..7798
                     /locus_tag="ISB17_002667"
     CDS             7064..7798
                     /locus_tag="ISB17_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAP1980582.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7800..8411)
                     /gene="emtA"
                     /locus_tag="ISB17_002668"
     CDS             complement(7800..8411)
                     /gene="emtA"
                     /locus_tag="ISB17_002668"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAP1980583.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8511..9425
                     /gene="ldcA"
                     /locus_tag="ISB17_002669"
     CDS             8511..9425
                     /gene="ldcA"
                     /locus_tag="ISB17_002669"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAP1980584.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            9520..11256
                     /locus_tag="ISB17_002670"
     CDS             9520..11256
                     /locus_tag="ISB17_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAP1980585.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11313..12383)
                     /gene="dadX"
                     /locus_tag="ISB17_002671"
     CDS             complement(11313..12383)
                     /gene="dadX"
                     /locus_tag="ISB17_002671"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAP1980586.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12393..13691)
                     /locus_tag="ISB17_002672"
     CDS             complement(12393..13691)
                     /locus_tag="ISB17_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAP1980587.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEALSVARYSRGFTPSRPGHLHGAHS"
     gene            14021..15553
                     /locus_tag="ISB17_002673"
     CDS             14021..15553
                     /locus_tag="ISB17_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAP1980588.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(15605..16324)
                     /gene="fadR"
                     /locus_tag="ISB17_002674"
     CDS             complement(15605..16324)
                     /gene="fadR"
                     /locus_tag="ISB17_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAP1980589.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            16546..18087
                     /gene="nhaB"
                     /locus_tag="ISB17_002675"
     CDS             16546..18087
                     /gene="nhaB"
                     /locus_tag="ISB17_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAP1980590.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLVEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            18233..18763
                     /gene="dsbB"
                     /locus_tag="ISB17_002676"
     CDS             18233..18763
                     /gene="dsbB"
                     /locus_tag="ISB17_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAP1980591.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(18808..20076)
                     /locus_tag="ISB17_002677"
     CDS             complement(18808..20076)
                     /locus_tag="ISB17_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1980592.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(20076..20495)
                     /gene="umuD"
                     /locus_tag="ISB17_002678"
     CDS             complement(20076..20495)
                     /gene="umuD"
                     /locus_tag="ISB17_002678"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1980593.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            20692..>21061
                     /locus_tag="ISB17_002679"
     CDS             20692..>21061
                     /locus_tag="ISB17_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HAP1980594.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETARFYVDYDDLMLSLLKE
                     AAQKMINTCNEYQKRHGKKTL"
BASE COUNT         4987 a         5512 c         5322 g         5240 t
ORIGIN      
        1 tcttgtggaa caaagtgtta tttaagaaac aagcaaatca ttctgtctct ttagacaaat
       61 tttgacatca aacatgatct ttttcgctat atgtgtaagt atatagcggt tttaattatc
      121 aggatattac ttatgtcact aaccagacgg aggtttacac agattcttgc gtcgacattg
      181 ttcctgcatc atctgccgtc ctttgcacaa tcagtcaaat tctgggcctc actgacgctt
      241 cccgaagccc aacacattac acgcatcgtc agcgcaggcg cgcccgccga tttactattg
      301 ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt ttgattttgc ccgtcaggca
      361 ttaattccat tgccagagca cattcgccag ttccccaggc tgggacgact cgccgggcgc
      421 gccagcacac tctcgctgga agggcttatg gcgttacatc ccgatttggt cgttgattgc
      481 ggcaatacgg atgaaacctg gatctcccag gcacggcagg ttagcgaaca gacacaaata
      541 ccctggttat tgcttaacgg gaaactggaa caatcagcag aacagttaac aacgcttggc
      601 aaaacgttag gcgaagagca ccgcgccgca gaacaagcca atctcgccag ccgcttcgtt
      661 ggtgaagctc aggcattcgc cacctcaccc gccgctaatc tcagctttta tgctgcgcgc
      721 ggtcctcgag ggctggaaac gggcttacag ggttcgttgc ataccgaggc ggcggaatta
      781 ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg gtctgacaca ggtttccatg
      841 gaaaatctcc tgcgctggca gccggatatt attctggttc aggaggccgt tactgcagat
      901 tttattcgtc gtgatcctct ctggcagggc gtgaaagcgg ttgcggaaca acgcatctta
      961 tttttaagtg gcctgccctt tggctggctg gatgccccgc cgggaatcaa ccgtcttctg
     1021 ggattacgca gacttcacgc ctggctggat cccgccatca atcgccagtt taaaagtgac
     1081 atgcagcatt acgcccaact gttctggcat tgttcactca gtgacgccga ctatcaaaaa
     1141 ttggtggcga gctaatgaga accgtaaacg gatgtatttt gctgctggca gctatcagta
     1201 tcacttttgc cgccgtaagt ggggcttatc atctcgatat gcagcaactg ctggcgctca
     1261 ttcttcgtca agaaaatgtt cctgtgcagg aacaaattgt cttctggcaa atccgcgtgc
     1321 cgcgtattct tgctgcgctg tttctgggcg cggcgctggc aggggccgga accacctatc
     1381 agggaatgtt ccgtaatccc ttagtgtcac cggatatttt aggcgtctcc gccggtgccg
     1441 gacttggtgc ctgcgcggca atcctgtggg gattatccat tgtttacatc cagctgtacg
     1501 cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt gattacgcgt cgggtgaccc
     1561 gccacgatcc gatcttaacc ctggtgctgg taggcattgc gttaggaacg ttgtgcggag
     1621 cggggatatc gctaataaaa acgctggccg atccttacac ccagttaccg tcaattacct
     1681 tctggttgct gggggggctg tccaccgtga ccttacgcga tctctgctat gctgcaccga
     1741 tcattctcat aggctccttg cccctgtttt ttctccgctg gcgcatgaat ttactcacgc
     1801 tttccgacga cgaagcccgt tcgctgggac tgaacgtgac acgtctgcgc tttggattga
     1861 ttgtctgcgc cacactcatc accgccagca cggtagcaat agcaggcata attggttggg
     1921 tcggcctggt cgttccgcac attgcccgct tgttgaccgg tcataaccat cagcagctgt
     1981 tgcccatggc aatgtgtact ggcgcgattc tgctcctgct taccgatacg ctggcacgca
     2041 gcattggaac aacagaaatc ccacttggca ttctgaccgc ttttgttggt gcaccgtttt
     2101 tcctgttttt gttgttacgc ggaggtcgcc agtgactttg cttgccgtcc gtcatgcgtc
     2161 tctgggttac tcccgccatc cggtgctacg cgacgtgtcc ttcaccctgt cgcaaggaac
     2221 aatttgttgt ctgttaggag ccaatggttg cggcaaaacc acactcatgc gctccattct
     2281 tggcgttata ccgttattaa aaggcgagat cttactggat agcctccctg tgcagacact
     2341 cagccatcgc cagcgcgcgc aggctattgc ctgggtgccg caggcgcacg acggcatttt
     2401 tgcttttagt gtgctggata tggtgttgat gggcctggcc cccaccatag gcgctttttc
     2461 cgttcccggt aagcaagaac gcctgaaagc aacggagcaa ctggaaaagc tgggcatttt
     2521 gcatctggcg acacgacgct ggaatacgct tagcggcggt gaacgtcagc tggctttaat
     2581 cgcccgtgca ctcgtccagc aaccgcgctt gttattactg gatgagcctg cctccagcct
     2641 cgatttcggt catcagatcc aacttctgga cacgctggcg cagctcaaaa ataacggcat
     2701 gacaatgtta atgtcgactc atcatccgct ccatgcaaac gccattgccg acagcattat
     2761 ccaggtagag ccagatggac gagtaacaca aggcttaccg accgaacagt taacaacgaa
     2821 aaaactggct gccctgtacc gggtttctgc cgaccagatt caccatcacc tctctgcaat
     2881 taagcactaa ggaaaaatca tgctcattga cgatattgat tttgcagacc tttaccttca
     2941 gcaactcagg ctggcccatc gcacagaaaa aacgccggat cactgggatc aacgggcgga
     3001 aaaaatggcg gaaaattgcg cctcgcctac ggacagctac ctgcaacaac tgacctcaaa
     3061 aattgacctg caaggcgcgc aaaccttatt cgatatgggc tgcggaccgg gtaccgtatc
     3121 gctggcgctg gcggacaaac tcacgacgat ttatggcgtt gattacagtc aggggatgct
     3181 gaacgttgcc gcccgtcgtg ccgccgcgct aaaggcagac aatgtccact ggatccagcg
     3241 cgcctgggaa gaggactgga gtgacctgcc acgctgcgat attgccgtgg cttcccgctc
     3301 cactctggtg gctgatatgc gccaggcaat gagcaaactg aataatcagg cacggcttcg
     3361 cgtctatacg actcatctgg tcagcacctc gttcgtctct ccagccattc agcgggcggc
     3421 tggtcgggaa gtgatcgaat tacccaacta tatctttgca ctcaacgtcc tgtatcagat
     3481 gggaatttat gctcacgttg attttatccg cggacaaaat tgccaacagg ataacagcac
     3541 ctgggaacgt tttgagcaga acgtcagctg gagtctgggc gcgcttaacg acgatgagcg
     3601 tgagcggtta tatcgctggt atcaacaaca agatgctcgt gccctcgccc ctgccagccg
     3661 cgactgggcg ctcatctggt gggatagcgt accgcaggag acactgcgat gattttcctt
     3721 tctcaggcac aaatcgatgc gttattgctg gaagatatcc agggcggtga cctgaccacg
     3781 cgagcattaa atattggaca ccagcatggc tatatagagt tttttctccg tcagggcggc
     3841 tgcatcagcg gcatttctgt cgcgtgtaag atgttaacta cgctgggatt aactattgat
     3901 gacgcggtca gcgacggttc acaagcgaac gcgggtcagc ggctaatccg tgcgcaaggt
     3961 aatgccgcag cacttcatca gggatggaag gcagtccaga atgtgctgga gtggagttgc
     4021 ggtgtttctg attatctcgc tcaaatgctg gcgttacttc gtgaacgtta ccctgatggc
     4081 aatatcgcct gcacccgaaa agcaattccg ggcacccgct tactggcctc gcaggcaatt
     4141 ctggctgccg gaggactgat tcatcgtgcc ggatgtgcgg aaaccatatt attgtttgcc
     4201 aaccaccgcc attttcttca tgacaatcag gactggtcag gcgcaatcaa tcagttacgt
     4261 cgccacgcac ccgagaagaa aatagttgtt gaagccgaca cgccgaaaga ggcaatcgcc
     4321 gcgttacgcg cgcaaccaga cgtacttcag ctcgacaaat ttagtccgca acaggcaact
     4381 gaaattgcgc aaatagcacc gtcgctggct ccccactgca cgctggctct gaccggcgga
     4441 attaatctga caacactcaa aaattacctc gactgcggca ttcgcctttt tattacctcc
     4501 gcgccttatt acgcgacacc agctgacatt aaagtcagtc tgcaacccgc agcctctatt
     4561 taattctttg ttaaacggaa tttattctat gagactcaaa aaacgttatc tctgcacagc
     4621 attatcactt gcctttaccc agcaggccgt agcggctcag gagagtgaca cgctgaccgt
     4681 atggtccagt ccggtatcat caacgacgac caccgttctc gatcaaccca ccatgaaggc
     4741 cctggataaa cagaatgtcg ctcaggcatt aagtgtcgtc cccggcgtgg tgctgcaaaa
     4801 gtcaggtagc cgcaacgaag aacaggtcaa agttcgtggc tttgatagtc gtcaggtgcc
     4861 ggtctatttc gacggtgtgc ccatttatgt tccctatgac ggcaacctcg atctggcgcg
     4921 gattctgacc aacaatttgg gggcagttga agtttccaaa gggtattcgt cgctgcttca
     4981 ggggcctaat cagatgggcg gagccattaa tatcaccacc cagaagccaa caaaacctct
     5041 ggaagcaagt ctgggatatc gccagggatg gagccgtagc caggacaatg cctacgatat
     5101 gcatgcttca tttgccgcca gcagcgatct ggggtatttg caagtcagcg gtagccagct
     5161 aaagcaggat tttctcggcc tgccgcatgg tgtaaataat gatattgcag gcaaacacgg
     5221 caagatgatt aattcatcgg ctgatgataa acgcggcatt gtgaagctcg gttttacacc
     5281 acgcgaaaac gatgaataca cattgactta cattaagcag gatggtgaaa aagataaccc
     5341 gccatacagt ggaaatagtg gtcagaaatc acgctactgg cagtggccag agtatgacaa
     5401 agaaagtttt tattatcagg gaacgaccca actaaacgat cgttttaccc tgaaaagtcg
     5461 gctgtatcgc gacacctttg aaaatacgct aatgatgtac aactcgctgg ctgatttgaa
     5521 aaataaaaaa ggcagctaca gccattattc cgattacagc gacggtgccg ggttacaact
     5581 ggcagccgat gtgcgtgaaa acgatctgct gtcgtttgcc gttaactgga aagatgacgt
     5641 acaccgggaa aaaggtgcgc cgcacgccgc ttacgatcgc tatgaagatc gtacctggtc
     5701 gctcgccagt gaatatcaat gggctgctgc cgataatgtc gatgtcgtgg ctggaatcag
     5761 ctatgactgg cgcgatagcg tagaagcgaa aaaacatgag aaagatggca gtatcaccca
     5821 ttatgacgac aacaatcagt cagcttttaa ctggcaggtg atggggaaat accactttgc
     5881 caatgaagac acgctggcgc tttcgtacta tgaccgcaca cgctttccga cgctgaaaga
     5941 acgctatacc acgtccaaac ctgcgtataa ccagatagcg attgttaacc cgcagctcaa
     6001 gccggaacgc gcgcgcgggg tggatttaac ctggaatggt gccttcacgc acgactgggg
     6061 atttgaggtc agcgtttact ataaccgggt gagtgatgcc atcctctcgc acaatatcga
     6121 tgccgatacc attcaaaatc agaacagcgg cacggtggat tacagcggtc tggatgccgg
     6181 tattaagggg aaaatcagca atatactgga tgtaggattg agctacgccc tgatccacgc
     6241 tgatgccaaa cgcaaagaca tcggcaagat aaccgatctg ccaacgcaga caatgaccgc
     6301 atggatgact ctcaaaccgt gggagccgtt aagcgtaacg ctgtcggaag aggcgcgttc
     6361 ctccagctac agcaacagtg acggttcaca aaaagccgcc ggttttgcgg tgactcacat
     6421 tcgagccgat tacaccttag gtcatggctt cagcgttaat gcgtcggtca ataacctgtt
     6481 tgataccaaa tatgcctaca gtgaagggtt tattgaagaa ggtcgcaatt tctgggcagg
     6541 tatcgagtat acgttctgat gtgaaagcgg atagcggcat ctgtatcgct atccgcctta
     6601 tgaaaatctt attctcttcg caggaggcgg aatacgccca gcaccaggat agcgcccacc
     6661 accgccacca ggaagctgtg caaattaaaa ccgctgatgg agccgccaat gccaaacatg
     6721 gtcgccagcc agccgccgac caccgcaccg actatcccaa gaatacaggt caggaaaaat
     6781 ccaccaccat ctcgccccgg catgattagc ttggcgataa tgccggctat caggccaaaa
     6841 ataatccagg caataattcc catgttcagg ccctcgtttc gatgaatcgg acgcaagaaa
     6901 tgcttacgtc aacgctctaa gtataggcaa gggcgggatc gatgctgggc atatgtcggc
     6961 tgatttaaga acaacatgta aaaaatattc gcgcatttgt attaagtttt gttaactgcg
     7021 accgataaac caaagacagt ttgtcagtca ggagtttttc cgcgtgagtc attaccatga
     7081 gcagttcctg aaacaaaatc cgttagccgt cctgggcgtg ttacgcgatt tgaacaaagc
     7141 cgcaattcct ttgcgtatca gctggaatgg cgggcagctg atcagcaaaa tactggcaat
     7201 aactccggat aaactggtgc tggatttcgg cagccaggcc gaagacaaca tcgccgtgct
     7261 aaaggcgcag cacattacca ttaccgccga aactcagggt gcgaaagtcg agtttactgt
     7321 tgaacaacta cagcagagtg aatacttgca gcttccggca tttattaccg taccgcctcc
     7381 caccttatgg tttgtacaac gacgccgata tttccgcatc accgccccac tccatccgcc
     7441 ttatttttgc cagaccaaac tggcggataa cagtacgtta cgtttccgcc tgtatgattt
     7501 gtcgttaggc ggcatgggcg cattactgga aacagcaaag cctgccgaat tacacgaagg
     7561 catgcgcttc gctcagattg aagtcaacat ggggcaatgg ggtgtttttc actttgacgc
     7621 ccagttaatc tccatcagcg agcgcaaagt gattgatggc aaaaatgaaa ccatcaccac
     7681 tccccgtctg agcttccgtt ttcttaacgt cagcccgacg gtggagcggc aattacagcg
     7741 gattattttc tctctcgagc gagaagcccg gaaaaaagcg gacaatgtgc gtgactgatt
     7801 tacatcgcgt ccagtgcctg ctcaagtttg tagatatagc gcggagcctg cggcgcagga
     7861 tgatttcgcg ctacgtgatc gaggaactca tcagcatcca gatcgttgat tttgctgatc
     7921 gcctttttcc gatctgacga gaaagtccgt agcagcgcac ctgccccgtt agcgtatgac
     7981 accaccagcg catattgcag taccttcgga tcttcaatgc ctgccagcgg gccggtttcc
     8041 agaatattca ggtaagccgc tcccattgaa atattacgct ccggattttt cagctcgctg
     8101 gtcgtcggct caccgctcca gcccatacga cgatatacat cacgtccgga ggttgaagct
     8161 tttatctgca tcaaaccaat ggcattcgat ttactcaccg cgttcggatt accacccgat
     8221 tcgatagcga taatcgccgt gatcaattgt ggatcgacgc cccaggctgc accggctttc
     8281 tggcttattg gcatccactg catcgcacgt tgcaccggaa ctttcgcgtt ccacggcggg
     8341 ttcgtatagt catgctttga tgaacaaccc gctaataaca caatcaaaaa ggcaaaccat
     8401 ctcaatttca cttcttctat ccttatagcc tggaatcgcc gttgaggtga caacggcaca
     8461 cctaagcggc atgatataca tttcgtttct gttgtcagca aggaattgcc atgtctctgt
     8521 ttcacttaat tgccccatcg ggttactgca ttaaacagca cgccgcgctg cgtggtatcc
     8581 agcgccttac cgacgcgggg catcaggtca ataacgtcga ggttattgcc cgtcgttgtg
     8641 agcgttttgc gggtacggaa acggagcgtc tggaagatct caattctctt gccagactga
     8701 ctacccccaa caccatcgtg ctggctgtac gcggcggtta cggtgccagt cgtttactgg
     8761 cagatattga ctggcaggca ctggtggctc gccaacaaca tgacccgtta ctcatttgcg
     8821 gacatagcga ttttaccgcc attcagtgcg gtcttctggc gcaaggcaat gtcatcacct
     8881 ttagcggccc catgctggta gcaaactttg gcgcggatga actaaatgcc tttaccgagc
     8941 accacttctg gctggcgtta cgcaataaaa cgttcaccat tgaatggcaa ggggaaggcc
     9001 cgacatgtca gaccgaaggc acgctgtggg gaggcaatct tgcgatgctg atttcactga
     9061 ttggtacacc gtggatgcca aaaattgaga acggtattct ggtgctggaa gatattaacg
     9121 agcacccttt ccgcgtcgaa cgcatgctgt tgcagctcta tcatgctgga attttgccac
     9181 gtcagaaggc gattattctc ggtagcttta gcggcagcac acctaatgat tatgacgcag
     9241 gttacaacct ggagtcagtg tacgcgtttt tgcgttcccg cctgtcgatt ccactcatta
     9301 ccggcctcga ttttggtcat gaacaacgca cggttacgct gccgttgggc gcacatgcca
     9361 ttctgaataa caccgaggaa ggcactcagt tgacgattag cggtcatcct gttcttaaaa
     9421 tgtaaaaaaa tcacgcttca gggctaagta aaacgctgtc tctgccgcta atatggtagg
     9481 gtttatatca gagacagcgt aatcaggagt aaccgaccgt tggatgccac aacaataatt
     9541 agccttttca tcttaggatc catccttgtc accagcagta tattacttag ttcattttct
     9601 tcccgtcttg gcattcctat tctggttatt tttctggcga tcggcatgtt ggcgggggtt
     9661 gatggcgtcg gcggtatccc gtttgataat taccccttcg cctacatggt aagtaacctg
     9721 gcgctggcga ttattttgct cgacggcggg atgcgcactc aggccagttc ctttcgtgtg
     9781 gcgttaggac cggcactgtc gctggcgacg ctgggcgtac ttatcacctc tggtttaacc
     9841 ggcatgatgg cggcgtggct gtttaatctt gatttgattg aaggattatt aatcggcgct
     9901 atcgtcggct ccaccgatgc tgcagcggtc ttttctctgc tgggtggtaa ggggcttaac
     9961 gaacgtgttg gctcgacgct ggaaattgaa tccggcagta atgatccaat ggcggtcttt
    10021 ctgacgatta ctctgattgc gatgatccag cagcatgaaa gcagtgtcag ctggatgttc
    10081 gtggtcgata ttctgcagca atttggcctc gggattgtca tcgggcttgg cggtggttat
    10141 ttactgctgc aaatgattaa tcgcatcgcc ctgcccgccg gattatatcc attgctggca
    10201 ttaagcggcg gtattttaat ttttgcttta accacggcgc tggaaggcag cggtattctg
    10261 gctgtttatc tgtgcggttt tctgctgggt aatcggccga ttcgcaaccg ctacggcatc
    10321 ctgcaaaatt tcgacggcct cgcctggctg gcgcaaatcg ccatgttcct ggtgctgggg
    10381 ctattggtta acccaagcga tctgctgcct attgccattc cggcgctcat tttgtccgca
    10441 tggatgattt tcttcgcccg tcctctttcg gtatttgccg gattgctacc gttccgtggc
    10501 ttcaatctgc gtgagcgcgt gtttatcagc tgggtaggat tacgcggcgc ggtgccgatt
    10561 attctggctg tgttcccgat gatggcgggg ctggagaatg cgcgactgtt ctttaatgtc
    10621 gccttctttg tggttctggt ttcactgcta ttgcagggaa catcactctc gtgggcggcg
    10681 aaaaaagcca aagtggtcgt tccgccagtg ggacgtccgg tgtcacgcgt tggcctggat
    10741 attcatccgg aaaatccgtg ggagcagttt gtttatcaat tgagtgccga taaatggtgc
    10801 gtgggcgcgg cactgcgtga tttgcatatg ccaaaagaga cgcgtattgc ggcactgttt
    10861 cgtgataacc agttgcttca tcccaccggc agcacccgac tgcgcgaagg cgatgtgttg
    10921 tgtgtaattg gtcgggaacg cgatctcccg gcgctcggta aactgttcag ccagtcgccg
    10981 ccggtcgcgc tggatcaacg cttctttggt gacttcattc tcgaagccag cgccaaatat
    11041 gctgatgtgg cgctgatata tggtctggaa gacgggcgag aatatcgcga taagcagcaa
    11101 acgctgggtg aaatcgtcca gcagttgtta ggcgcggcac cggttgtcgg tgaccaggta
    11161 gagtttgccg ggatgatctg gacggtggcc gagaaagaag ataatgaagt gttgaagatt
    11221 ggtgttcggg tagcggagga agaagccgaa tcttaagttt aatgtgtttc gtaacgctgg
    11281 atacgacgct ggtcgcatcc agcgataaca agttacaccg tcacaaccgg gacgcgtaac
    11341 gccagcgcgc acatcaactc atagcctacc gttccggcag cggcggcgac atcatcaatt
    11401 ttgatctcct tgccccacag ctcaactggc gtaccaatac ccgcttgcgg gcaaggcgtt
    11461 aaatcgaccg ccagcatatc catcgagact gtccccaccg tcatggtgcg aacgccgtcc
    11521 actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta tcctgcggcg
    11581 acaatgccga ttcgctgttc atcgcgcgca gtatagcgac cgccgtagcc cacacgctcg
    11641 cccgctttta gcgtctggac accaataatc tcactgctta gcgtcatcac cggacgtaat
    11701 ccggtattgg cgatatcacg ccactgaccg gacggcgaag caccatacaa aataatgcca
    11761 ggccgaaccc agtcaaaatg cgcttcctgg tgccacagag tcgccgccga attagacaac
    11821 gaacgccgac actccagccc ttccgccgcc tgctcaatac gcgccatcgc gctggaaatt
    11881 ccatcaggat gttccgcttc ggcaaaatgc gacatcaggg tcatttcgcc aacattcgcc
    11941 atcgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc caaccgattc
    12001 atcccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc attttgcagt
    12061 gctttgagct gccagttgct gtgtacgcag gtggtcaggc ggtgctggtc ataaatctcc
    12121 agatcctgag catggaaaaa tccttccagc atcaggatcg gtcctttcca gccgcgctca
    12181 cgtaacgtta ttgcctcttc caggttaagc aatgcaaagc catcggtagc cccgagcgcg
    12241 ctccagatac gctcaatacc gtgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc
    12301 gcgtgcggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg cagatcgagg
    12361 ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg taaatggccc
    12421 gggcgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagagcttc atacgggatc
    12481 gctggcgtgc gccccgagag cagatcgctt aacaactgac cggaaccgca agccatcgtc
    12541 cagccgagcg tgccgtgacc ggtattcaac cacagatttt taaagcgtgt acgcccgaca
    12601 accggcgtgc cgtctggcgt catcgggcgc aggccagtcc agaaagccgc ctgatcaaca
    12661 tgaccgccgc gtggatagag atcgcgaacc accatctcca gcgtttcacg acgcggttgc
    12721 aacagctcgg tgttaaaacc aacaatttcc gccattccgc cgacacgaat gcggttatcg
    12781 aaacgggtaa tggcgatttt gtaggtttca tcaagaatag tggataccgg cgcaccatct
    12841 tcctgcgcaa ttggaatggt cagcgagtag cctttcagcg gatacaccgg aatatcaaca
    12901 atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc atcggcctta
    12961 atcacttcat caccacactt cacgccgtag atttgctcgc cgtcgcaaag cagttggtca
    13021 acgggcgtat taaagcggaa tttaaccccc gcctgctccg ccatccgcgc cagattctgg
    13081 gtaaacagct ggcagtctcc ggtttcatca ttgggcagtt gcaggccgcc cgtcagtttg
    13141 tgcgctactt ctgccaacgc gggctccact tccgccaggc ggctggattc cagcagctga
    13201 tacggtacgc cagcatcttc cagcacggcg atatcgcggg tcgcgttttc atactgttgt
    13261 tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat attggtttcg
    13321 gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgtaccat ccgccctttg
    13381 ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca tttcaactgg
    13441 aactgcgtac cgtcgagacg aaccgccagc ggcgcatggc gctggaacat ccatttaatc
    13501 gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga gatttgcccg
    13561 gcattcgcgg cactggtttc cagggcggcc cccggctccc gatcaatgac ggtgacctca
    13621 tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact tcccagtatg
    13681 acaactcgca tagccactcc gataacggta gatacagtaa aagaataata atctaattac
    13741 atcttgataa ctcagttgaa aatattattc aacatacggc ttttttatgg tgagctggct
    13801 cacatctccc tgactctgcg gcaatatggc gtctttagta tctcctgctg aacgcccttt
    13861 ttatccagca taaaattccg ttcagaagcg gattagtggc actctgataa taagaaatcg
    13921 caaagaaaaa aattctccgg cgacgcggtt tttaacaggt gttctatgct tgaaatgagg
    13981 tgctccacac agagagcgcc gacaacaatg aggtgcgcgt atggcgacga tcgattctat
    14041 gaataaggac accacacgtt tgagcgatgg acccgactgg acgttcgacc tgctggatgt
    14101 ttatctggca gagatagacc gggtggcgaa actctaccgg ctggatacct acccgcacca
    14161 gattgaagtg ataacctcag aacagatgat ggatgcttac tccagcgtcg gcatgccaat
    14221 taactatccg cactggtcat tcggtaaaaa gtttatcgag actgaacggc tgtataagca
    14281 cggtcagcaa ggactggcct atgaaatcgt cattaactct aacccgtgta tcgcttacct
    14341 gatggaagag aacaccatta ccatgcaagc gttggtgatg gctcatgcct gctatgggca
    14401 taactctttc ttcaaaaaca attacttatt ccgtagctgg accgacgcca gttcgattgt
    14461 cgattatctg atttttgccc gtaaatatat taccgagtgc gaagaacgtt atggcgttga
    14521 tgaagtagaa cggcttctgg actcgtgcca cgcgctgatg aactacggcg tggaccggta
    14581 caaacgcccg caaaaaatct cgctgcaaga agagaaagcc cggcagaaga gtcgcgaaga
    14641 gtatctgcaa agtcaggtca atatgctctg gcgtaccctg ccgaagcgcg aggaagagaa
    14701 aacggttgct gaagcgcgcc gctatccgtc cgaaccacaa gaaaacctgc tctattttat
    14761 ggagaaaaat gcgccactgc tggaatcatg gcagcgtgaa atcctgcgta ttgtgcgtaa
    14821 ggtgagccag tatttttatc cgcaaaaaca gactcaggtg atgaacgaag gctgggcgac
    14881 cttctggcac tacaccatcc ttaaccatct gtatgatgaa gggaaagtaa cggaacgttt
    14941 tatgctggag tttttgcaca gccacaccaa tgtggtcttc cagccgccct ataacagccc
    15001 gtggtacagc ggcatcaacc catatgccct cgggttcgcc atgttccagg atattaaacg
    15061 gatttgtcag tcgccgacgg aagaagacaa atactggttc ccggatatcg ccggttccga
    15121 ctggctggaa acgctgcatt tcgcgatgcg tgatttcaaa gatgagagtt ttatcagcca
    15181 gttcctgtca ccgaaagtga tgcgtgattt ccgcttcttc accgtactgg atgatgatcg
    15241 acataattat ctggagattt ccgctattca taatgaagaa ggttatcggg aaatccgtaa
    15301 ccggttatcg tcgcaatata acttaagtaa tctggagccg aatattcaga tctggaacgt
    15361 ggatttgcgc ggtgaccgtt cgctgacgct gcgttacatt ccacataatc gcgcaccgtt
    15421 ggatcggggg cgcaaagaag tgctgaagca tgtgcatcgc ctgtggggat ttgatgtgat
    15481 gctggaacag caaaacgaag acggcagcat cgagttgctg gaacgttgcc cgccgagaat
    15541 gggaaatctg taaaaacaac gaaaaacccc tcgtttgagg ggtttgcttt ttaaacggaa
    15601 gggattatcg tccctgaatg gctaaatcac ccggcagatt tttctgcatc cggtgccaaa
    15661 tctcgccact ctcatgccca tagcgacgca ctgtttcata tacctgatcg tgtgcgcctt
    15721 cactgcacaa cgccgacagt ttgtgataga agcccagcgc cagactgcgc gcttccggat
    15781 tggcgaaata gtgacgacca atgcgcgtat acagcccttt catcccgtta aggatcagac
    15841 cgtaaatcgg gttgccggaa gcaaacgcca ggccgcggaa aatgttgtaa tccagctcgg
    15901 caaaggcgtc ggcgtgatcg gccacttcat tagcggttgc cagcacttcc tgcgctttat
    15961 cgggatgttg acgaaacgcg gtgcgaataa aaatagttga aatattggta cgcaccgaca
    16021 gcaaattatc aataagctgc ggcacacttt cgtggtccag tcgcgccagt gtttcaagga
    16081 tatttaaacc agaggtttcc cagaaattat tcaccttcgt cggcttgccg tgttgaatgg
    16141 tcaaccagcc atctcgtgcc agacgttgta acacttcacg taacgtagta cgcgttacgc
    16201 caattaattc tgaaagttca cgttctgcgg gcaaaatagt cccgggaggg aagcggttat
    16261 tccagatact ttcaataatg tactcttccg cgaaacccgc cgggctttgc gccttaatga
    16321 ccatagtgag atttccataa cacagcaaaa caaagttgga ctcatcatac cagagggcta
    16381 actacgctga tagaagacaa aaagaagaaa aaagggatca aggcttcatt ttctgcatct
    16441 ctttcccctt gactatcaag acttgcctca ctcctgtctg accgttaagc tttgcgttta
    16501 aacataaaaa catcattgat tatttctaag gtaagggaaa ccatcatgga gatctcctgg
    16561 ggccgcgcac tatggcgcaa ttttttgggc cagtcccccg actggtacaa actcgccctc
    16621 atcattttct taatcgtaaa tccgttaatt ttcctcatca gccctttcgt cgctggctgg
    16681 ttgctggtag cggaatttat tttcactctg gcgatggccc tgaaatgcta cccgctgctc
    16741 cccggtggtc tgttggctat cgaagcggta ttcatcggca tgaccagcgc ggaacatgtt
    16801 cgtgaagaag tggcggcaaa tcttgaagtc ttgctgttac tgatgtttat ggtggcgggt
    16861 atctatttta tgaaacagct gttgttgttc atatttaccc gtttgctgtt aagcattcgc
    16921 tccaaaatgc tgctgtcgct ctctttttgc gtggcggcgg cgctcctctc cgcgttcctc
    16981 gatgccttaa ccgtcgtggc ggtggtgatc agcgttgcag tcggttttta tggtatttat
    17041 caccgcgtag cctcttcccg tactgaagat accgacctgc aagacgatag tcatatcgac
    17101 aagcattaca aagtggttct ggaacagttc cgtggctttc tgcgtagtct gatgatgcat
    17161 gccggtgtcg gcaccgcatt aggcggcgtg atgaccatgg tgggcgaacc acagaacctg
    17221 atcatcgcta aagcggctgg ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg
    17281 accgtcccgg ttctgatttg tggcctgtta acctgcctgc tggtagagaa gctgcgttgg
    17341 tttggctacg gtgaaacgct gccggagaaa gtccgcgaag tactgcaaca gtttgacgat
    17401 caaagccgca accagcgtac ccgtcaggat aaaatccgtc tgattgtcca ggcgattatc
    17461 ggcgtctggc tggtgactgc gctggcactg catctggtgg aagttggttt gattggtttg
    17521 tcagtcatta ttctggcgac atcattgacc ggtgttaccg atgaacatgc tatcggtaaa
    17581 gccttcaccg aatctctgcc attcaccgca ctgttgacgg tgtttttctc agtagtcgcg
    17641 gtgattatcg accaacaact gttttcgccg attattcagt ttgtgttgca ggcatctgaa
    17701 catgctcagc tgtcgttgtt ctatattttc aacggtctgc tgtcatccat ttcggataac
    17761 gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc
    17821 acgttgaagc aatatgagct gctggcggtc gccattaata ccggtaccaa tctgccctcc
    17881 gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca
    17941 ttgattcgtc tctcttatgg ccgcatggtg tggatggccc tgccttacac cctcgtactg
    18001 acggttgccg ggttactctg cgtcgagttt acgcttgctc ctgtaaccga atggtttatg
    18061 caaatgggct ggatagcaac gctttgataa caacttaccg ggcatataaa tgcccggttt
    18121 gccttttcgc ccgataattg tccaattgcg tcctattttt gctgcctcct ggtggcgcag
    18181 cgaatgaatt ggtttaaact gcgcactcta tgcatattgc agggaaatga ttatgttgcg
    18241 atttttgaac caatgttcac aaggccgggg cgcatggctg ttgatggcgt ttactgctct
    18301 ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct
    18361 ctgtatttat gaacgctgcg cgttattcgg cgttctgggt gctgcgctga ttggcgcgat
    18421 cgccccgaaa actccgctgc gttatgtagc gatggttatc tggttgtata gtgcgttccg
    18481 cggtgtgcag ttaacttacg agcacaccat gcttcagctc tatccttcgc cgtttgcgac
    18541 ctgtgatttt atggctcgtt tcccggaatg gctgccgctg gataaatggg tgccgcaagt
    18601 gtttgtcgcc tctggcgatt gcgccgagcg tcagtgggaa tttttaggtc tggaaatgcc
    18661 gcagtggctg ctcggtattt ttatcgctta cctgattgtc gcggtgctgg tggtgatttc
    18721 ccagccgttt aaagcgaaaa aacgtgacct gttcggtcgc taatccattc ggcgctcctg
    18781 cgggagcgct ttttcctgcc gctatgttta tttgacccgc agtaaatcag aacttcgcgt
    18841 tgtataacat ggtgaaagca tggcacgctt catctgccat tgttgctgga tcccctgccc
    18901 ggcaaaatag agtgttcctc tgccctcttt agcattcagc gtatccatta ccgccatcaa
    18961 ttgctcactc ccggggcgtg gtgcgttgtc atcgaataaa ttgagctgcg cgactccctg
    19021 actgaagaaa tcccccagca tcacgcccgc tttttggtag cgatggcccg cttgccagat
    19081 ggcatccaga gatcgcgtag cggcgttaat gatatccctg ctgtcctgag tgggcgtcag
    19141 cagttttacc gatgcgctat tgccgtaata aggttcattg aacgcaaatg gtgacgtctt
    19201 aataaacgtg gagataaacc gacaatattg atgctcgctg cgaagttttt ccgccgcccg
    19261 ggcagcgtaa ctacaaatgg cctgccgcat cgacggataa tccgtgatgc gttcaccaaa
    19321 cgagcgggaa cagataattt cctgcttcgt cggtgcaaac tcttccagtt gcaaacaggg
    19381 ttcaccgcgc agttcacgca ccgttctttc gagcacgaca ttaaaatgtt tacggataaa
    19441 ccggatatct gaatctgcca aatctagaac ggttttgatc cccatcgcgt ccagtttttt
    19501 gctgatccgc cgtccaatcc cccagacttc atccacgggg agagcagaca ttaatttacg
    19561 ctggcgttcc agatttgata aatccaccac cccacccgtc tgccgctgcc atttttttgc
    19621 cgcatgattg gcaagcttag ccagcgtttt ggtctgggcg atccccacac caacagtaag
    19681 atgggtacgt tgtagcaccg ttgcgcgaat ttctctgcca aaatcagtca gatcgcgaca
    19741 attatgcaca cctgtcagat cgcagaatgc ctcatcaata ctgtaaatct cgacgcgggg
    19801 cgatagttct tccagcgtcg acatcacccg attactcatg tctgcgtaaa gctcataatt
    19861 gctgctaaag caaaccacgc cacagcgacg aaacagatct ttttgtttga accagggatc
    19921 gcccatttta acgcccagcg cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt
    19981 attcgatagc acaaccaccg gtttacccca taaatcaggg cgaaacaccg tctcacagct
    20041 ggcataaaac gcgtttacat cacagagggc aaacatcagc gcatcgcctt aacgacgtgg
    20101 atcaccacac caaagacatc cagcgtatct tcgctactga tggtaatggg cgagtacgca
    20161 ctgttcatag gaataagctg taccgtcggg cgtagttgca attttttcac cgtaaactcg
    20221 ccgtcaacag cagcgatgat aatatcacca tggctggcgg taatagcgct atcgacaatc
    20281 agtaaatcac cgtcactaat cccaccatca atcatagaat caccacttgc tttgacgaaa
    20341 taagtcgcgc tgggatgctg gatcaacagt tgattcagat cgatgcgctg ttcaacgtaa
    20401 tctgcggccg gtgaaggaaa gccacactga acaagatcgc taaatagcgg aaaagtcaca
    20461 atttcgcgga gatccgcagg cttgataaac aacataataa tctgcctgaa gttatactgt
    20521 ttttatatac agtagtctgt tcttgtcagc agatcaatac tgattcaggc tatcaatatt
    20581 tgtcgctgca tagactgctg atttttcgtt ctcttatcct gtgctcacgc ggccttctgg
    20641 cgacagcgtc catccagcag aaaggaaaaa tatcacctgg tttaaaatag aatcgaagca
    20701 ttcgcccaga aaggcattct cattaaggta ctggatgacg gcatcaagaa actgtgttct
    20761 attgctgcgg gcgtagttga agggaaactg attccagaat tgaagaacaa gttaaaatct
    20821 gtgcagagtt tctttaccac cctgtctaac acggttaaac aagcgaataa agatatcgat
    20881 gccgccaaat tgaaattaac caccgaaata gccgccatcg gggagataaa aacggaaact
    20941 gaaacagcca gattctacgt tgattatgat gatttaatgc tttctttact aaaagaagcg
    21001 gcccaaaaaa tgattaacac ctgtaatgag tatcagaaaa gacacggtaa gaagacactc
    21061 t
//
LOCUS       DACADA010000074        20627 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.075, whole genome shotgun sequence.
ACCESSION   DACADA010000074 DACADA010000000
VERSION     DACADA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.075"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            345..978
                     /locus_tag="ISB17_002680"
                     /pseudo
     CDS             345..978
                     /locus_tag="ISB17_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(1342..1417)
                     /locus_tag="ISB17_002681"
     tRNA            complement(1342..1417)
                     /locus_tag="ISB17_002681"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1382..1384),aa:Thr,seq:cgt)
     gene            complement(1532..2785)
                     /gene="proA"
                     /locus_tag="ISB17_002682"
     CDS             complement(1532..2785)
                     /gene="proA"
                     /locus_tag="ISB17_002682"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP1980595.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2797..3900)
                     /gene="proB"
                     /locus_tag="ISB17_002683"
     CDS             complement(2797..3900)
                     /gene="proB"
                     /locus_tag="ISB17_002683"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP1980596.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4189..5244
                     /gene="phoE"
                     /locus_tag="ISB17_002684"
     CDS             4189..5244
                     /gene="phoE"
                     /locus_tag="ISB17_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP1980597.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5283..5684)
                     /locus_tag="ISB17_002685"
     CDS             complement(5283..5684)
                     /locus_tag="ISB17_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP1980598.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5742..6986)
                     /gene="frsA"
                     /locus_tag="ISB17_002686"
     CDS             complement(5742..6986)
                     /gene="frsA"
                     /locus_tag="ISB17_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP1980599.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7078..7536)
                     /gene="gpt"
                     /locus_tag="ISB17_002687"
     CDS             complement(7078..7536)
                     /gene="gpt"
                     /locus_tag="ISB17_002687"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP1980600.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7797..9254
                     /gene="pepD"
                     /locus_tag="ISB17_002688"
     CDS             7797..9254
                     /gene="pepD"
                     /locus_tag="ISB17_002688"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP1980601.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9310..9924)
                     /locus_tag="ISB17_002689"
     CDS             complement(9310..9924)
                     /locus_tag="ISB17_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAP1980602.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9921..11072)
                     /locus_tag="ISB17_002690"
     CDS             complement(9921..11072)
                     /locus_tag="ISB17_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAP1980603.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11250..11702)
                     /locus_tag="ISB17_002691"
     CDS             complement(11250..11702)
                     /locus_tag="ISB17_002691"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1980604.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11699..12754)
                     /gene="dinB"
                     /locus_tag="ISB17_002692"
     CDS             complement(11699..12754)
                     /gene="dinB"
                     /locus_tag="ISB17_002692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP1980605.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12825..13748)
                     /gene="lafU"
                     /locus_tag="ISB17_002693"
     CDS             complement(12825..13748)
                     /gene="lafU"
                     /locus_tag="ISB17_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP1980606.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(13751..14614)
                     /gene="motA"
                     /locus_tag="ISB17_002694"
     CDS             complement(13751..14614)
                     /gene="motA"
                     /locus_tag="ISB17_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1980607.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14627..15343)
                     /locus_tag="ISB17_002695"
     CDS             complement(14627..15343)
                     /locus_tag="ISB17_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAP1980608.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(15363..15830)
                     /locus_tag="ISB17_002696"
     CDS             complement(15363..15830)
                     /locus_tag="ISB17_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAP1980609.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(15838..16899)
                     /locus_tag="ISB17_002697"
     CDS             complement(15838..16899)
                     /locus_tag="ISB17_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAP1980610.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16896..17207)
                     /locus_tag="ISB17_002698"
     CDS             complement(16896..17207)
                     /locus_tag="ISB17_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAP1980611.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17212..17604)
                     /gene="fliS"
                     /locus_tag="ISB17_002699"
     CDS             complement(17212..17604)
                     /gene="fliS"
                     /locus_tag="ISB17_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1980612.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17627..18943)
                     /gene="fliD"
                     /locus_tag="ISB17_002700"
     CDS             complement(17627..18943)
                     /gene="fliD"
                     /locus_tag="ISB17_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1980613.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19353..20267)
                     /gene="lafA"
                     /locus_tag="ISB17_002701"
     CDS             complement(19353..20267)
                     /gene="lafA"
                     /locus_tag="ISB17_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HAP1980614.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         4786 a         5596 c         5137 g         5108 t
ORIGIN      
        1 tgtgaaatca tatgccccac tcctgatgtt gtggcgttat tcgagaataa aatgatgcca
       61 atttataata aactggcatc aaacactcaa gagaatagtc atttaatcca actccgcgac
      121 tggctcctcc ctctacttat gaacggacag gtcacggtca aataatcccc ggcgtggttt
      181 tgcgaggcgc attccagatc cgcattgcgc cgcactcacc gcagcaaaac cgcttgccgt
      241 tcataaccga tcgcagtatc tttctcccgc gcctgcaaaa tcaggcgtcg ggattagcct
      301 cctgaaaacc gttacaggcg acacttgacg caccagcgtc ttttttgtgt cgtgcgttcg
      361 gctacacctc aatggcgggc cgggcggggg cgtcgcaaga cgcgccggtt tcctgtaacg
      421 ccggtaaggc taaccccgtc cggttccgcc cccatgagat tagcctctct agtggcggac
      481 ataataaacc gttacaggag acgccctatg gctacaatcc tcatctctga ctgcgattta
      541 ctcgatctat tgtccgctcc cttcaatgcc accacggatt tcaccgtcct ggccgactac
      601 tgcgcccgct tcgccgaaac cctgatagaa accgacgatc cggcgctgcg gctggcgctc
      661 tgcggacggc ttcacgcggc tcttgcgcta ttgcaaccca cgctgcacga ccccatcccg
      721 ccgcatctga ttgaaagcct gaccgttgat acccttcccg ccacaccaga gcactttatt
      781 tccgaaccag attccttgtg cgaatactgc ctcgcgctgc tccagctact gctggaacgt
      841 ccagcagagc caaaaacgga acaaaccctg accgggcttc tgtgtgagct ggtctggaat
      901 ttcacggaag gactcaaatc cccgcgctgg ctgcgtaccg ccgacggcgt aaaatttatc
      961 agtgaggtgg ccgcatgatg caacaggact gacattccgc cgacattatt gcggtgctga
     1021 aaaagcgggg aacgcctctg gcggcacaga gaaaaactca ttgcggaagc gctgtaaata
     1081 tcgccagaaa tcatctggtc ctcaaggtac tttgatagca aaggcaagct ggctggttca
     1141 gtccggagtc tctcttcttg cacttaaaga gatgggagga tgggaaactc ttgaaatggt
     1201 tcgtcgttag gctcaccttt cagccaggca tttcaccggg cacgcgagca aaatcgatgc
     1261 gattataagt cgcaatggca caaatacggc acaagaggag aacgtggtgt atttaaatat
     1321 ggggtaacta cttgatttta atggtgccga taataggagt cgaacctacg accttcgcat
     1381 tacgaatgcg ctgctctacc aactgagcta tatcggccct gaaaggacat gttcacgaac
     1441 gtgaatcacg gtggacaagg ttaaaactaa ccgggcgatg cgtcaatggc cttgtgaatc
     1501 aaatggctac ttttgcatca cccggtttta tttacgcacg aatggtgtaa tcaccaatgc
     1561 cgatccactt gtaagtggtc agtgcttcca gccccattgg gccgcgcgcg tggagttttt
     1621 gtgtgcttac cgccacttcc gcccccagtc caaactggcc gccgtcggta aaacgcgtag
     1681 aggcgttaac gtaaacagcg gaggaatcca cttcgttaac aaaacgctgg gcgttgcgca
     1741 tatcgcgggt caggatcgca tcggagtgtt gcgtgccgtg ttcacgaata tgggcaatgg
     1801 cgtcatccag atcgctaacg attttgacgt tcaaatctaa tgacagaaac tcatcgtcat
     1861 actcttcggc tttaacagcc accaccttcg cggggcctgc ctgcaactgc gccagcgcgg
     1921 ctgtatcagc gtgtaacgtc acgccgcttt ccgccatttg tttgcttaat gcgggcagga
     1981 agctatcagc gatgttttta ttcaccagca acgtttctac cgtattacac gtgctcggac
     2041 gctgagtttt cgcgttgaca attactttca gggcttcagc gatctctgcg ctttcatcaa
     2101 cgtaaatatg gcatacgcct ataccgcctg tgatcaccgg gattgtcgac tgctcgcggc
     2161 acagtttatg caaaccagcc ccgccgcgcg ggatcagcat gtcgatgtat ttatccatac
     2221 gcagcatttc actgaccagc gcacggtcag gattatcaat cgcctgcacg gcacccgccg
     2281 gtaagccaca agagttcagg gcgtcctgaa tcaccgccac cgttgcagcg ttagtgcgac
     2341 acgtttcttt gccgccgcgc aggatcactg cgttacctgt tttcaggcac agcgaagcga
     2401 catcaaccgt cacgttcggg cgcgcttcat aaatcacgcc aataaccccc agcggtacgc
     2461 gacgacgctc aagacgcagg ccgctgtcca gtacgccgcc atcgattacc tgccccaccg
     2521 gatcggcgag gttgcacacc tggcgcacat catcggcaat gcctttcagc cgtgcgggcg
     2581 tcagtgtcag acggtcaagc atcgcttcgc taaggccatt ggcacgcgcg tctgcaacat
     2641 cctgcgcgtt agcgttgagg atactttcgc tttgtgcttc cagttcatcg gcgatttttt
     2701 ccagcacacg atttttttcg cggctggaga gttgcgctaa tttatacgag gcttgcttcg
     2761 cggcaatgcc catttgttcc agcatcggcc agctccttaa cgggtaatca tgtcatcacg
     2821 gtgaacggca accgggccgt attcatatcc cagtattgca tcaatttctt gcgagtggtg
     2881 tccggcaata cggcgtaatg catcgctgtt gtaacgactg acgccgtggg cgatatcgcg
     2941 accttcgagg ttgcaaatac ggatgacttc accacgcgag aaattgccag tcacgctttt
     3001 aatgcctttc ggcaacaggg agctgccgcg ttccagaatg gcggcagttg ccccttcatc
     3061 taccgtgatt tcacccgcag gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg
     3121 agtcgcctgg gcatggaaca gcgtaccgac ggaaatgcct tccatcacat caccaataac
     3181 gcccggcttg ctgcccgcgg caataatggt gtcgataccc gcacggcaag ccacgtcagc
     3241 ggcctgcaat ttggtgctca taccgccagt tccgaggcct gaaacgctat caccggcaat
     3301 cgcgcgcagt gcgtcatcaa tgccgtacac atctttaatc agttctgcct gcggattgct
     3361 gcgcgggtca gcggtataca aacctttttg atcggtcagc agcaacagtt tatcggcacc
     3421 cgccagaatc gccgccagcg cagaaaggtt atcgttatca ccgaccttaa tctctgccgt
     3481 agcgacagca tcgttctcat taattaccgg aacgatattg ttatcgagca acgcacgcag
     3541 ggtatcgcgg gcgttcagga agcgttcacg atcttccata tcagcacggg tcagcaacat
     3601 ctgcccgaca tgaatgccgt aaattgaaaa cagctgttcc cacagttgaa tcagtcgact
     3661 ctgccctacc gccgccagca gctgttttga agcgatagtc gctggcaatt ccgggtaccc
     3721 caggtgctca cgtccggcgg caatcgcacc cgacgtcaca ataacaatcc gatgcccggc
     3781 ggcatgtaac tgcgcgcact ggcgaacaag ttcaacgata tgggcacggt tcaggcggcg
     3841 cgatccgcct gttagcacac tggtgccgag ttttaccacc agcgtctggc tgtcactcat
     3901 gattctctgc cattcaattt taggaaaaaa tgatatcaaa cgaacgtttt agcaggactg
     3961 tcgtcggttg ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag
     4021 cgtagcggca gttgtttacg cttttattac agatttaata aattaccaca ttttaagaat
     4081 attattaatc tgtaatatat ctttaacaat ctcaggttaa aaactttcct gttttcaacg
     4141 ggactctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc
     4201 actctggcat tagtggtgat gggcattgtg gcatctgtat ccgtacaggc cgcagaaata
     4261 tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg
     4321 agtgataatg acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa
     4381 acacaaatta acgatcaact gactggttat ggtcgttggg aagctgagtt tgccggtaat
     4441 aaagcggaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg gttgaaatat
     4501 aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga cgtggaagcc
     4561 tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg
     4621 accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat
     4681 ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa
     4741 aacggcgatg gcttcggcac gtcattgaca tatgactttg gtggcagcga tttcgccatc
     4801 agtggagcct ataccaactc tgatcgcacc aacgagcaga acctgcaaag ccgtggcaca
     4861 ggcaagcgtg cagaagcatg ggcaacaggt ctgaaatacg atgccaataa tatttatctg
     4921 gcaactttct attctgaaac acgtaaaatg acgccaatct ctggcggctt tgccaataag
     4981 acgcagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg
     5041 ggttatgtct tatcgaaagg gaaagatatt gagggcatcg gtgatgaaga tctggtcaat
     5101 tatgtcgatg ttggtgctac gtattatttc aacaaaaata tgtcagcgtt tgttgattat
     5161 aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga tattgtcgcg
     5221 gttggcatga cctatcagtt ttaatggata ttgccggatg tgatgcatcc ggcacatttc
     5281 attcacgccg ttaacttcac cggttcatca cgaaaatcat ctgccggttc cagcttcaga
     5341 ttcagcgtcg ccagcagctc acgcagcttc tcgtgaaatt cacgcagggt gtgctccagt
     5401 cgttcaacca cttcggcatc ttttatctga acactcgtcc agtcgcctgc tttatcgaac
     5461 agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttccagttc catccaccag
     5521 ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg
     5581 aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag tgcggtaaat
     5641 tttttgacta atctgctctt cgggtgtcca ctcggtaacg tcattgcaat ctcctgttgt
     5701 gatgcaactg ttttaccaaa ttggtaaaat ttagcaaatt tttaacacaa acgtttttcg
     5761 atccagtctg tgatttcctg aaggcctttg tcaaaattcc gatacaccgg gttaaatggg
     5821 atctctaata atttaccgtc agcagatgac gacgtgatta agcgtgagtc ctcttccggg
     5881 ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg acgtcccagc
     5941 aacccttgca cttttaatga atagcgattc agctccacgc gcaacgcttc gtcagaagca
     6001 tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac ctgttgctgg
     6061 cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac cgctttcaga
     6121 cgcggcgatt caaggtatgc cagacgtacg gcaacgttag cgccgaaacg gaaaccaaag
     6181 gccgcgacgc gagtgtgatc cacccacggt acgttaggca acgcctttaa aacgtgctga
     6241 tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac cgacggcata
     6301 tcaatagtca gcatcgcaat gccgcgcagc gcaaaataac gttcatacag gctgtaatag
     6361 tctgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg gccatcgcct
     6421 ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc
     6481 cgcatcgtgc ccggcagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc
     6541 gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cagcaatgtt gtacaacgta
     6601 gccgcatgca gccagtgtcg accgctgagt tgcggatctt cttccgcaca ggctttttgt
     6661 tgccacacca tcgcctgggt agcccactca taaatccagt tgccgccgcg gtagccaatc
     6721 accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgcgcctgg
     6781 acttcgagga tttcacgggg gtcaatgccg cgccagatcc acatcagacg gttaatcatg
     6841 cgataccagt gagggatggt tttaccatca agggccgact gcacaggcag ttgtgcgccg
     6901 tgactaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg
     6961 gtttcgctca ggtttgcctg tgtcatagat gcagcctcca gaatacttac tggaaactat
     7021 tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta
     7081 gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt
     7141 attttgcggg atatcaacaa cataatcatc aaccagcgga cgaccagccg gttttgcgaa
     7201 gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt
     7261 atctaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac
     7321 tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg
     7381 aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat
     7441 aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg
     7501 caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc
     7561 tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc
     7621 actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc
     7681 gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc
     7741 tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt
     7801 ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta
     7861 ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag
     7921 agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta
     7981 ccgcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc
     8041 aaaaaaataa cgacaccgtg catgacttca ctaaagatcc tatccagcct tatattgatg
     8101 gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct
     8161 ccgcactggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga
     8221 ccatgactga agaagccggt atggacggtg cgttcggctt acagagcaac tggttgcagg
     8281 ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg ggatgtgcgg
     8341 ggggtatcga cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg
     8401 aaaccttcaa tttaacctta aaaggtctga aaggcggtca ctccggcggt gaaatccacg
     8461 ttggcctggg taatgccaac aaactgctgg tgcgcttcct ggcgggtcat gcagaagaac
     8521 tggacctgcg tcttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag
     8581 cctttgcgac cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtggata
     8641 cctatcagga gatcctgaaa aacgaactgg cagaaaaaga gaagaatctg gcattgctgc
     8701 tggactccgt agcgaacgac aaagctgcat taacggcgac atctcgcgat acctttattc
     8761 gtctgctgaa cgccaccccg aacggagtta tccgtaattc cgatgtagcc aaaggcgtgg
     8821 ttgaaacctc cctgaacgtc ggtgtggtga ccatgaccga caataacgta gaaattcact
     8881 gcctgatccg ttctctgatc gacagcggta aagactacgt agtgagcatg ctggattcgc
     8941 tgggtaaact ggcaggtgcg aaaaccgaag caaaaggcgc atatcctggc tggcagccgg
     9001 acgctaactc tccggtaatg catctggtac gtgaaaccta tcagcgtctg ttcaacaaga
     9061 cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc
     9121 cggaaatgga catggtgtct atcgggccaa ccatcaccgg tccacactcc ccggatgagc
     9181 aagttcatat cgaaagcgtt ggccattact ggacactgct gactgaactg ctgaaagaaa
     9241 ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaacgcctt ccggcagttt
     9301 catccttcat tatccttcga taaaagccat ccctgtgaac gttcgtcgcg ggttgccacg
     9361 ttcaatctgg tgatggaaca ttcgccgctg cgatttcagc gccgcgctat tttcctgttg
     9421 ctgttgttcc agcttccagg caatcagtaa tcgcgccagc cgtttgttag catgctgact
     9481 gcgctctgac tgaaccttca cgctaatacc ggatgccaga tgcgtggcgc gtaccgccga
     9541 gtcggtttta ttgacatgtt gaccgcccgg ccccgacgaa cgcagcgtct cataacggat
     9601 tgcatccgat tgttcctgct catcagcggt aaaacgccca atgcccagaa accagttttt
     9661 gcgcccatga tgaggccgat acggactcgg acaaatccac tgaatagtgc cgcaccagct
     9721 ttcgcttaga gcccatgcgt tatcgccatc cagagaaatc agcgccgaac gcagtgtgtc
     9781 agagtagcga cccgtttctg tttccagcac cgttaccgcg acgtcctgtc gggtagcttc
     9841 tttaatcagc ctgtccagtg cttttttcac tgcgagacaa cattcttccg gcccctgagc
     9901 agaggagagt tgtagcaaga tcatcctttt ttccctccac tgtttttgag cgtcagcacc
     9961 gggcgcagtc gcgcgacagg aataattaac ccagccagca ccagacattg caccacgctt
    10021 tcagccgatt tataggcttg tggcgcttct tcaaagatga gttgtttatc gcgacaaatt
    10081 acccgactgc caagttcagt ccgcgagagt tgcgtcgccg tgtatttcgc tgccagacgc
    10141 cctttacact cggtgcgccc ccatttacgc cccgccccat gcgccagcga gtggagcgtt
    10201 ttttcgttcg caacaggttt aaccagccag gagtaatcgc cgcgtgaacc gggaataatc
    10261 accagaccgt tgtcatccgg cgtggcgcct ttacgatgca accagccctg ttgatcgccg
    10321 atttggcacg cgctaacaaa gttatgcgcc acatccagaa ccggacttcc ggtggcctta
    10381 acttgttgca ttatgcgcaa agcgatcagc tggcgattaa tacgcgcaaa cgccagcgca
    10441 tcgtcatgtt ccgcaatata ccgtagcgcg tcgtcactgc cttcaggcaa accatgatgc
    10501 gaaaacgagg caatatgccg ctgtaaaata gactgcccca atcccctcga gccgctatga
    10561 accagtagtt gcagatgctg cgcatccaga ccagcccgtg caaacagttc cgcgtcgata
    10621 atttgatcaa tctgttgcag ttctgcgaag tggttaccgc caccgatgga gccaagcgag
    10681 ttgtgccacg gatgctgtgc aaatgctgac ggcaggtttt cctccagcca gctttcttca
    10741 gcaacgtcat ccagatctga taatcgcttt tcaaacttat cggcgttgta tttgcgagcg
    10801 agaatatctg tttgccatag cgccatgccg cagccgatat cattgccgac cagtgccggg
    10861 taaaaacgac caacagagaa gaacgctgcg ccaatcgggt agccgcgtcc gggatgtaaa
    10921 tctggcatcc ccacaacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg
    10981 atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc atcggataag
    11041 ggacgaatat atttgcccat gtaccattcc atttatgaat aaaaatcagg aaatggcaga
    11101 cgaatccagg gcaagaaaaa agaattaatc ctggaaaggt ctgcaaagag tcattgataa
    11161 aggatttgtc atgatttacc tccttgcagt ttgtggaata agaaaacgcg cacatactag
    11221 acgggcgtta ttttcattgc aagctggatt taatgttgcg gtttatatcg catataaaaa
    11281 ttagtaaacc acgttccccg gcattcaacg cgctgctgct taactgtctg aaaaccataa
    11341 cgttcaaaaa agggttttgc ggttatgctg gcgtccaccg taagttcgga ttcagactta
    11401 atcaatggtt ttaacaaagc gctggcaact ccgcgtcggg tgtattccgg ttcaacaaat
    11461 aacatatcga tataatgtcc aatgcgggta ataaaaccaa ccggttgtgc attaataact
    11521 gcaacccgca cttgtgattt cgcgagtttc tccttccagc gagattcgtc aatctgcgcc
    11581 caggcggcaa tttgctgtgg agaatagtgc tgactcgccg tcatcgtaac agctcgaagg
    11641 aaaatagcgc atagttgctg aaaatcacca ggctgatagt ttcttatttg tatgttattc
    11701 ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca tgcagcccca
    11761 ccagtcgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg gtggcgatta
    11821 gatcagattt attcagccgc ggccagacgt gttcctgggt ggtttgctga aaatcgtcga
    11881 actttaattt caccccctgg cgggcaatca gcaaatcagg ttttaccttt gccaaacgac
    11941 gctccagttc agggtaaagc cgctcgatga tcgcttcaca ttcagaccag tgatgaatat
    12001 cttccgccat cgtacgttct acaccgacgg attttcgcaa cctttcgctg ttaacgtcgc
    12061 gttcgtcaat gccctgacta cgctcccaca aaatgcggcc aaatttgcca aagcgtttaa
    12121 gcagcatcac cagatcacac ttttgcacat caccgcaggt ccgcagcccc atcgcttcca
    12181 gttttgccgc cgagactttg ccgacgccgg gaatttttgc cagcggtaag gtttgtaaaa
    12241 atgtcggaac ttctgccggc gtaatcacaa actggccgtt gggtttattc atgtcggagg
    12301 cgattttggc gagaaacttt actggtgcca cgcccgcaga cgccgtcagt tgcagctcgt
    12361 tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg caatggacgc
    12421 tatcggtgac atcgagatag gcttcatcca gtgataacgg ttcaatgcgc gaggtatagc
    12481 gcgagaaaat ttcgcggata tgatttgagg cttctttgta ggcgtcaaag cgccctggaa
    12541 gcaaggtgag atgcgggcat aatttgagcg ccatccctgt cggcatagcg ctacgtacgc
    12601 caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgtcgttcg cggctgccgc
    12661 caatagcaat agggatatcg cgcagggcgg gattgtcgcg catctccacc gctgcgaaaa
    12721 agcagtccat atccacatga atgattttac gcattgctca cctctcaata ctggtaaagt
    12781 atacagtgat ttcagggttt gagaaatgcg taaagattca gcatttaacg cgttcgctgc
    12841 gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt atccccatgc
    12901 tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat aatctcaatg
    12961 cgccggttgc ccgcgctttg cggatttttg gcatccagca gcatctggtc cgccattgca
    13021 cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg acgagctgaa
    13081 agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta ggccatcgca
    13141 tcggtatgcc cggtaataat aattttgtta tcgagcgagt cgaacactgg cgcaagctcc
    13201 accagcaaag ttttaaagaa cggcataatc ttcgcgctgc cgcgttcgaa catattgcgg
    13261 ttctggtcgt ctttaatcag cacgcgtaat ccctggggaa caatctccat ctccagattc
    13321 gcttccatat gcgcatcacg ggcgatggta ttgatactgg ttgccagctc gccaagctcg
    13381 gtggcggatt tcttcttcag cagcgccgtt ttctcgttga cgtcgcgcgc ttttttctcc
    13441 gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct gattttattt
    13501 aacggcgaca acccaccacc gttaaaaatc gactgcccgt gcagcgcggc gataatgctt
    13561 tcacgctcgg atttactgac gctgttgaca atccacagcg tcataaatag cgccatcatt
    13621 gccaaagtaa agtcggcgaa cgccaccttc cacgccccgc cgtgatgccc ggcatgattc
    13681 tttttgatct gccgccggac gatggtggtt tttgccccac gttcgcggtg gttgcccgtc
    13741 tttctcatgc ttattcttgc tccagcatcg cattcaccca cgcgtcgagg ttggcgaagg
    13801 tcggtttgga cgccagatgc agcagcttac gcccggcgtc caccgccagc agcgtcggtt
    13861 tgccgcctgc ctgcgccacc agcacagtgc gcacgcattc cagcaaagaa tgttccgccc
    13921 gcgcctgctg ttccatggcg ttggctagcg ggtccatcag gcagtagcag ataaacacgc
    13981 ctaaaaacgt ccccaccaga gccgccgcca ctttcaggcc aatcagagcg atagatccat
    14041 caatcgactg catggtgatg atgatgccca gcaccgccgc gcaaatacca aatcccggca
    14101 tcgcttcggc ggtacgttgc agcgagcgcg acggcgttaa cagggactct tccgcggtgt
    14161 ccagttcctg atcgagaatg ccttccagct catgggcgtc gattttaccc atcgccatca
    14221 ggcgaaagtt atcagcaata aaggtcacca gccgcttctg ggttaacacc agcgggtatt
    14281 tctggaaaat ggtgctctct tccggctgct caatatgctg atccaacatc cgcagaccgc
    14341 cgttttgcac catttcgagc aactcataca ggcacattaa tagctgacgc tgaaactccg
    14401 gcccgagctg ttttttactg atcacgcctt taatttgatg ggcaatctct ttcagcacat
    14461 gtttcgggtt accaataatc atcgccccga aaccggcacc caaaataatg atcatttccg
    14521 ccggttgcca gatagccact aattgcccgc ccgcttcaaa ataaccgcca aatacgcagc
    14581 cgagcaccac cagtaaacct aatatttttt gcatgatttc ttcactttaa ttcggataga
    14641 caaaatcacg aattttctgc gcgatctgtt tattcagttg acaaattcgg gcctcggtta
    14701 gccctaatac cagcgcaatc tctttgagat tcatctcatg ctggtaatac atggagagga
    14761 tcagttgctc tttttcgctt aattgtgaaa gcgcgtgttg caacatttgc tgagtgacaa
    14821 actgatcttc cagttcccgg ccagcaagcg gaatgcctgg cccttcgccg ttcagcagtt
    14881 cgtccaggct ggcgagcgtt tccgcccctt ccagttgctg atattcgaga taatcttcat
    14941 gcgagatccc ttcctcagcc agctcctgcc actccggttc gtgccccagt tttttacgcg
    15001 tttcgcgaat caggtctttc atctgatgat atttctgtcg cagctggcgc ggtcgccagt
    15061 cgagagcgcg cagctcatcc aggatcgcac cgcgaatacg gtgcacggca tagcccgcaa
    15121 aaccttcgtc cggcagccca tagcgacgaa tggcgttcaa cagccccatt aacgccgttt
    15181 gttccatatc ctgacggtca ataatgcaat tacactgcgg ggcaagttgc cgcaccactt
    15241 tacgcaccaa cggtaaatag gcctgcaagt agtggctctc ctgctgcgga gttaatacca
    15301 gcgtggctgc aaattcttct gactcgaata catcctgtag catgtttata tccgcctggt
    15361 gattactgga agaccacttt tttcaccagc aaatccttat acggtgcggt cattttgcga
    15421 cgctgaagat ctttctttaa cgcgtcagaa atcagtttgc ggatggcaga tattttcatg
    15481 ccgcgcaaat cttcaaattt catttccgtc aggcattcaa ccgcaatact ttgatacagc
    15541 ggttcctgat gctttatttt ttccgcctct ttgtcactgg cgaccaccat cactaattcc
    15601 gccgacaaat aatgatccgc gccgttgttg tcatgcaaag tgacaatggt ttccggcagc
    15661 gtcacaaata cgctcttgct ctcatccaga gcttccaccg attccgtttg cgcggcggta
    15721 gtcggttttc cttttccggc ataatgatgc cagacactcc atcctgctcc agcgccgacc
    15781 accaacgcca gaacggcgct gaccacgcct gccaccacga ttttcttcat attgccttta
    15841 cactttgatc aaaacggatt cgttctgttg aaattgaggc gcatcatcaa gctgtaaggc
    15901 ggcactcacc tcttcctgct gatggcccga ctgttgttgc tgctgcgact gcccttcgga
    15961 ggaaacctgt acgttcacct ccataaaatt ttgcgccgtc agatgctggc ggagatcgtc
    16021 gctaaactgc tgcaatgcgc ggcagacttc gctttgattc gcaccgatat ggaccgtcag
    16081 tttgccgtta tccaactgta cggcgatctc aagcttgcca agcgacggtg gatcgagacg
    16141 aatggtggag atctgctgtt gtttgttgag ctgaaagtgg atctgatctt tcaacagcgt
    16201 cgtcagtttt tcgcccagtt cgtccatcga aagcgtggct ggtttggcga caatctcctg
    16261 atgatcgctg accaactctg gcgtcttaac ctgcaacacc gcacgcgcaa taaccggttc
    16321 gctgtccggc actttttccg ttttacgctc gacctgcggg cgcgcggtca cacgggttag
    16381 ttcaggtttc gggcgagctg gcggtgttga gacgcgcggc tgcgtcggcg caatggcatg
    16441 taaatcttca ctggcgtaag tcgccagtct ggcctgctgt tcgggtttcg cctgcggcaa
    16501 ctgaccgatc agttcctgca actgcggcgg caacgacgtg agttcctgct gctgcgtcgg
    16561 gcgttgcggc gcgttttgca ccaccgcttt cgtcaattgc tggataaccg gcgatgttgc
    16621 gacgtttcgc ggctgcggcg tgtcctgatg cggcgcggca ggttgcggaa gtaacagcgc
    16681 cattagcgcc tgcatcgcca ccggatcgtt ctcctgcggg cgcgtcgcct gttgctgact
    16741 ggttttagcg ctatgaacgc gctccgccac cgccgcgacc accattttcg gcagggtaaa
    16801 cacgggcgcg ttttcaccgc tcaccggcgg taaaatatcc gccttcaacg aagcggtttc
    16861 cgcaagcgtt ccgagcgtgg caagcaatgc cggattcatg acttctccga aagtaaatta
    16921 acaattgagt aggccagtac gccttcctga tgccgctgat gctgttccat acgcgctttc
    16981 aacacttctg cgcgctgctc gcgccgttta atcagggcat taaacgcgcc gtacagttgt
    17041 gccttcaccg catccaactg cgccgcgtcg gtttccgccg ccgcgtaatc gtgaaactgg
    17101 cgcatcaact gctgatacag tgccggaagg cgtcgccagc gttgcttgtc gagggcgtca
    17161 ttcatcgctt cgaccagtgc aaaaagttgc ctgcgttgtt tttccatcgt cttaacccag
    17221 cttgcccgaa agcccttccc agccttcgcg caggttgctt aagatcagca tcacttcatc
    17281 gattttctct gccgacagtt cgccgctggc ttcatacaag cgatacacac agtggtcata
    17341 gaggcgcgaa aggttgacca ccagctcgcc gccggtttca aattccagcg aactggtgag
    17401 cgcattgaga atgtcgatgc atttattgat gctctgggct ttcttctcga aacgccgccc
    17461 ttcaatgtgg cttctggcgc gttccagctc gtccatcagg ccagaaaaca gcaccagcac
    17521 cagttcaaga ggcgatgccg ccgcggtacg cgccgcgaga tcgatctctt tgtattgcga
    17581 ataaccgtcc tgtacttggt acatctttaa ggatattcct tctgaattac gcaaatgccg
    17641 ccatactggc tttcatggtg tacacctcaa ccaacgtatt ggtgtactcc tcgagataac
    17701 ggtcgtagtt agcgttatag gtatcggtaa gctgatcgcc ttcgtcctga attttgctca
    17761 tctgatcatc aatgttctgc tggcgcaggg tgatgatgcc gttgctggag tccgtgtagg
    17821 tgttaatcag gtcgtccatc tgcgccacca tgctgttgtc gccaacgaaa atagaggtca
    17881 ggccgtcggg atcgctcttc atcgcgtcgt tgaactggtc ggagtcaatc tgtaagtggc
    17941 cgtgagaatc gagcgtgatg ccatagtcga caatcgacac gccgttgtag ctggcatggg
    18001 cgatgtcatc gagctggttt gccagtgagc tgagtccagc atcccccgca aagaccccgg
    18061 cgctactact gtcatcaccg tgggtggtta gcgaatcaac cgtatcaatc aaggtgttat
    18121 aggcatcgac aaaggtctgt actttctcct ggctggcgct ggaatcttca ctgatgttaa
    18181 aggtggtgag atcgctgtca gaatcgctga cttcggtaaa agtcatggtg acaccgggga
    18241 tcacatcatc aaacgtgttg ctgctgttgg tgatagccgg tccgctggcg ctaccgagat
    18301 gaataatcgc gtcctgagcg gaggaaacat ccgtggcaac gggcgcactg gtgctatcgt
    18361 tgctggcatc atgccccgtt acgctcaccg aaaacgcgct ctgcgccccg gtgctatcgg
    18421 aggtgagcat aatcgtggtg gtgccgtcgg tttttaccag tgccgccgac acgcccggat
    18481 tgtcatcgga gtcgttaatc gcattcacca gctcgctggc gtcgataaaa ccgtcaccgt
    18541 caccgttctg atctgccgcc gacagatcga tatccatagt gctgtcgccc atcgtcagtt
    18601 caaaagtacc ggtagcggaa aaggcgtcgt cgcccatact gaacgtggtt tgctgcccct
    18661 gcgccagttg ctcaacgaaa aacgagtagc ttcccgcctg cgcctgggaa ttggcggaaa
    18721 cggtcgccga gtcattattg ctggtggcgg caaaagtcac cgggccgtcg gtatcgctgt
    18781 tcagcgcgtc aacggcgctc tggaaatcgc tcaacgccga gctgagcgag tccaggccgc
    18841 tgctttcagc atccagctca ctctgcttct cctgcaaatt cgtcgcctga gtagcgacat
    18901 ccgcatacgc gatttcttgc gcaatggttc ttgggttgat cattgatgac tccggataac
    18961 aatgtcaggt tattggcaaa aggcgtgcca atatttttta tgtgaataat caatagattg
    19021 aaaaacaggc ggaaatgacg tttccggtct ggcggaaaaa agcctgccgc agaaagcaca
    19081 acaccgccca caggcggtgt tgttcgtggc cggataaggc gtttacgccg catccggcta
    19141 tcggtgcaca gtgcctgatg caacgctgac gcgtcttatc aggcctacga gtccgcgcct
    19201 gtttttatgc gtgtgcccat ttgtaggccg gataaggcgt tcacgccgca tccggcaatc
    19261 ggtgcacaac gcctgatgcg acgctgacgc gtcttatcag gcctacgagt ccgcacatat
    19321 ttttatgctt gtgcccgttt gtcggcccga acttactgca gcaggctgga aaccatgctg
    19381 gacatgctgt tggactgttt cagcatggtg ataccggtct gcatcagcac ctgttgtttg
    19441 gtcatattgg acgcttccgt cgcgtaatcg gtatccatga tgttgccgat agccacttcg
    19501 gtgttgtcct gcatgctggt caggttgttg gcggtgtcat tcagacggtt gatagatgca
    19561 cccaacttgg actgaatctg agaaacgtca tccatcgcgg tagaaatgct ggtgatcatc
    19621 tggttagccg agccagacgc agtcagttca gtaccgccag aaacgcctgc ggtgccagtg
    19681 gtgtcagcct gatccgcact aaaggagttg ctgatagcag aaaggtcagt caccagcgtg
    19741 ttcagttggc ttgaaatgtt cacggtcatg gtgtcggaag attccgcgcc cacctggaaa
    19801 gttaccgcgc tctggaacag accgtcagta ccggtgttgc tggtgccgga aacaccgaac
    19861 aggttggtgc cgccgtaagt agtgttttgc agcatgtcag acatctgctg gcccagttcg
    19921 tcgtattcgt cctgcatcgc ctgcaggtcg tcgtcgctgt aggtgccgtt cgctgcctgc
    19981 gttgacaggt ctttcatacg ccccagcacg tcagacattt cgtcgaacat gctgtcagcg
    20041 gtttgcagca tcgcggtagc atcattgatg ttgctcagcg ccacacccat accgctggac
    20101 tgtgccgtca gacggttagc aatttgtttg cctgcggcgt catcggcaga agagttaatc
    20161 cggttacctg tcgccaggcg ttccatagaa gtagacagag aagagctgct tttgctgatt
    20221 gcattaacgg cggccattga ggcgttattg gtgttaatag ataacatgat actgctcctt
    20281 gggattaatc gtgttcgttt cgataccctg taaaaacgac acctcgggag caaacttaaa
    20341 atcgccgtta aaattttttc gccacgtatt atccgatgcc acttttagct aaaaaaagca
    20401 gagtttaaat tcagataaaa agattattag caatatctgc cacatggcca ggtatcaaaa
    20461 tatgtcttta tcaaatgctt gatatagtta agattgtcat caattaacgc gttttcttct
    20521 caatgtactg atgccaaaat ttacattgcg ttgcaaatag tacacgtttg gctattgtac
    20581 ctgtcctgtc atttcgttac atttccacgc catatagtga ttaagaa
//
LOCUS       DACADA010000075        20074 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.076, whole genome shotgun sequence.
ACCESSION   DACADA010000075 DACADA010000000
VERSION     DACADA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.076"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(4..96)
                     /locus_tag="ISB17_002702"
     tRNA            complement(4..96)
                     /locus_tag="ISB17_002702"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Ser,seq:gct)
     gene            complement(412..597)
                     /gene="csrA"
                     /locus_tag="ISB17_002703"
     CDS             complement(412..597)
                     /gene="csrA"
                     /locus_tag="ISB17_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAP1980615.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(832..3462)
                     /gene="alaS"
                     /locus_tag="ISB17_002704"
     CDS             complement(832..3462)
                     /gene="alaS"
                     /locus_tag="ISB17_002704"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP1980616.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3591..4091)
                     /gene="recX"
                     /locus_tag="ISB17_002705"
     CDS             complement(3591..4091)
                     /gene="recX"
                     /locus_tag="ISB17_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP1980617.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4160..5221)
                     /gene="recA"
                     /locus_tag="ISB17_002706"
     CDS             complement(4160..5221)
                     /gene="recA"
                     /locus_tag="ISB17_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP1980618.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5301..5798)
                     /gene="pncC"
                     /locus_tag="ISB17_002707"
     CDS             complement(5301..5798)
                     /gene="pncC"
                     /locus_tag="ISB17_002707"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAP1980619.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            5980..6219
                     /locus_tag="ISB17_002708"
     CDS             5980..6219
                     /locus_tag="ISB17_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980620.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            complement(6258..7343)
                     /gene="mltB"
                     /locus_tag="ISB17_002709"
     CDS             complement(6258..7343)
                     /gene="mltB"
                     /locus_tag="ISB17_002709"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAP1980621.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7600..8163
                     /gene="srlA"
                     /locus_tag="ISB17_002710"
                     /gene_synonym="gutA"
     CDS             7600..8163
                     /gene="srlA"
                     /locus_tag="ISB17_002710"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP1980622.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            8160..9119
                     /gene="srlE"
                     /locus_tag="ISB17_002711"
                     /gene_synonym="gutE"
     CDS             8160..9119
                     /gene="srlE"
                     /locus_tag="ISB17_002711"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP1980623.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9130..9501
                     /gene="srlB"
                     /locus_tag="ISB17_002712"
                     /gene_synonym="gutB"
     CDS             9130..9501
                     /gene="srlB"
                     /locus_tag="ISB17_002712"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP1980624.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9505..10284
                     /gene="srlD"
                     /locus_tag="ISB17_002713"
     CDS             9505..10284
                     /gene="srlD"
                     /locus_tag="ISB17_002713"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAP1980625.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10390..10749
                     /gene="gutM"
                     /locus_tag="ISB17_002714"
     CDS             10390..10749
                     /gene="gutM"
                     /locus_tag="ISB17_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAP1980626.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10816..11589
                     /gene="srlR"
                     /locus_tag="ISB17_002715"
                     /gene_synonym="gutR"
     CDS             10816..11589
                     /gene="srlR"
                     /locus_tag="ISB17_002715"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAP1980627.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11582..12547
                     /gene="gutQ"
                     /locus_tag="ISB17_002716"
     CDS             11582..12547
                     /gene="gutQ"
                     /locus_tag="ISB17_002716"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAP1980628.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12544..14058)
                     /gene="norR"
                     /locus_tag="ISB17_002717"
     CDS             complement(12544..14058)
                     /gene="norR"
                     /locus_tag="ISB17_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAP1980629.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14245..15684
                     /gene="norV"
                     /locus_tag="ISB17_002718"
     CDS             14245..15684
                     /gene="norV"
                     /locus_tag="ISB17_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAP1980630.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15681..16814
                     /gene="norW"
                     /locus_tag="ISB17_002719"
     CDS             15681..16814
                     /gene="norW"
                     /locus_tag="ISB17_002719"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAP1980631.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(17036..19288)
                     /gene="hypF"
                     /locus_tag="ISB17_002720"
     CDS             complement(17036..19288)
                     /gene="hypF"
                     /locus_tag="ISB17_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAP1980632.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19441..19968)
                     /gene="hydN"
                     /locus_tag="ISB17_002721"
     CDS             complement(19441..19968)
                     /gene="hydN"
                     /locus_tag="ISB17_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAP1980633.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4792 a         5562 c         5065 g         4655 t
ORIGIN      
        1 aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca tactccctta
       61 gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg agtgcttcga
      121 agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct gagacttata
      181 agtcaaacaa tttttcccac acttttatcg tttgcacact tcacgctcaa ttagtctgta
      241 aaaacgtcaa aaagagtgtt ttatcaacag aaaaacggag gtctgacaga tagcagtaat
      301 gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac aatggagtga
      361 aagagaaatc ttgagggtgc gtctcaccga taaagatgag acgcggaaag attagtaact
      421 ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt gaacagaaac
      481 ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta aaactgtcac
      541 ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa tcagcattct
      601 ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta tccatcatat
      661 aacgccaaaa agtaagcaat gacaaacaca ttacatctaa gcagtcatgg cattacattc
      721 tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg ttgtcgatag
      781 cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat attattgcaa
      841 tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg cagcatccgt
      901 accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct gctgagcgac
      961 cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata cgcctgcaat
     1021 cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc ccagctgatt
     1081 ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa gctcgctaac
     1141 caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc tctcctgtgc
     1201 ggcagcttgt tctttaagct gttgtaactc tttttccagc tgacgcgtac gttccagtac
     1261 tgaacgcact ttatcagcca gattattgct atcgcctttc agcagatgcg cgacttcgct
     1321 taagcgatcg ctgtctgcat gaacggtggc gatagcgcct tctccggtta ccgcttcgat
     1381 acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac caatatcacc
     1441 agtgcggctg gcgtgagtac cgccacacaa ctcggtggag aaatcaccca tgctcagcac
     1501 gcgtacgcgc tcatcatact tctcgccaaa cagcgccatc gcacctttcg ctttcgccgc
     1561 ttcgagatcc atgatgttgg tttcgatcgg caagttacgg cgaatctgcg cgttcaccag
     1621 gtcttcgacc gcacgaatct cttctggttt catcgcttcg ttgtgtgaga agtcgaagcg
     1681 cagcactttg tcgttaacca gtgaaccttt ctgcgataca tgagtaccca gaacctggcg
     1741 cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc
     1801 ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga gaacccacag caagtttacc
     1861 gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg agaagttagc
     1921 gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga atggcgtttg
     1981 atccagcacg accacagctt cctggcctgc attgatggca tcaaccgatt taccatcaac
     2041 aaacagcgca gtcactttgc cgttcagttc cagatggtca tagcctttaa attcagatgc
     2101 actgtcaaca cggatcattg cgttgtaatc tgcaccaaag ccgctggctt cgcgcgcacg
     2161 acgacgctgc tcttccattg ctgcgtcaaa accagcttcg tcaactttga tgttgcgctc
     2221 acggcaaacg tcagccgtca ggtcaaccgg gaagccgtag gtgtcgtaca gacggaaagc
     2281 agtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca gcagcgccag
     2341 accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct gctcaacctg
     2401 cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatta gcggaccaac
     2461 cagtttgtag aagaaggttt ctttcgcgcc gagcatatta ccgtgacgca ctgcgcgacg
     2521 aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc catccgcgat
     2581 caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat tgctcagatc
     2641 ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata
     2701 gttagagtta acgtgttgca gcaccgcagc aatacgttcc agacccatac cggtatctac
     2761 agacggtttc ggcagcggtt ccatcgtgcc atcggcctgg cggttgaact gcatgaagac
     2821 gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg gagggccgcc
     2881 ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc
     2941 acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat
     3001 aatacgttcg cgcgggatcc ctacttcttt ttcccagatt tcgtaggctt cgtcgtcgct
     3061 ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt cgctggtcag
     3121 cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc
     3181 cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca gatcgttgtg
     3241 tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat tacgcttgtc
     3301 gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca acagagttgg
     3361 gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac tatggaaaaa
     3421 gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc tgaaatcaag
     3481 ctaacgaaat atcgctacca gccgttgcgt gtcgttaacc gcccggctgg taactgaaaa
     3541 gtgggaataa gataagtttt cttgactggg aagtaaaatc ccgtatgcgt tcagtcggca
     3601 aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc
     3661 tggatcttaa ctttttctga aaagacagtt ggcaaaggtt cgccatattt tcgcgtcgcc
     3721 tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct
     3781 tcgcgggaaa tacctttctg attcagttcc tgacaaatac gcgcaggtcc ataacctttg
     3841 cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcaagata gccatgttca
     3901 tggcaccagg caataacgcg ctcgtaatct tctgccgtgg catcaatctc ttccgggcca
     3961 tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc
     4021 accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggatgtt
     4081 gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgaccct
     4141 tgtgtatcaa acaagacgat taaaaatctt cgttagtttc tgctacgcct tcgctgtcat
     4201 ctacagagaa atccggcgtt gagttcgggt tgctcagcag caactcacgt actttcttct
     4261 cgatctcttt cgcggtttct gggttatctt tcagccaggc agtcgcattc gctttaccct
     4321 gaccgatctt ctcacctttg tagctgtacc acgcgcctgc tttctcgata agcttctctt
     4381 tcacgcccag gtcaaccagc tcgccgtaga agttgatacc ttcgccgtag aggatctgga
     4441 attcagcctg tttaaacggc gcagcgattt tgttcttcac cactttcacg cgggtttcgc
     4501 tacccaccac gttttcgccc tctttcaccg cgccgatacg acggatgtcg agacgaacag
     4561 aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc cgggttaccg aacatcacac
     4621 caattttcat acggatctgg ttgatgaaga tcagcagcgt gttggactgc ttcaggttac
     4681 ccgccagctt acgcatcgcc tggctcatca tacgtgccgc aaggcccatg tgagagtcgc
     4741 cgatttcacc ttcgatttcc gctttcggcg tcagtgccgc cacggagtca acgacgataa
     4801 cgtctactgc accagaacgt gccagggcgt cacagatttc cagtgcctgc tcgccagtgt
     4861 ccggctggga gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg tagattgggt
     4921 ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc ttcgcgctgc gctgcggcga
     4981 tcacctgcaa cgtcagcgtg gttttaccgg aagattctgg tccgtagatt tcgacgatac
     5041 ggcccatcgg cagaccacct gccccaagcg cgatatccag tgaaagcgaa ccggtagaga
     5101 tggtttccac atccatggaa cggtcttcac ccaggcgcat gatggagcct ttaccaaatt
     5161 gtttctcaat ctggcccagt gctgccgcca acgctttctg tttgttttcg tcgatagcca
     5221 tttttactcc tgtcatgccg ggtaataccg gatagtcaat atgttctgtt gaagcaatta
     5281 tactgtatgc tcatacagta tcaagtattt tgtagaaatt gttgccacaa ggtctgcaat
     5341 gcatacgcag tagcctgacg acgcaccgca tcacggtcac cgctgaagca ttcccgccgg
     5401 gtaatgcctt caccacgggc agtggcaaaa gcaaaccaga cggtgccgac aggcttctct
     5461 tcactgccgc catccggccc ggcgatacca ctaatagaca cggcgtaatc agcacgagcc
     5521 gctttcagtg cgcctatcgc catttccacc acgacaggtt cgctcaccgc gccatgctgc
     5581 gccagcgtct cttcgcgtac accgatcatt tgcgcttttg cttcgttact gtaggtgaca
     5641 aatccgcgtt caaaccaggc ggaactgccg gcaatatcgg taatcacttt cgctacccaa
     5701 ccgccggtac aagactcagc agttgttacg gttgcgccac gggctttcag cgccagccca
     5761 acctgttcgc ttaactgcat cagttcactt tcagtcatca caacctctgt cagtcaaaaa
     5821 tttatgccgg acaagatagc actttcccgc aaaagatggg gacgagcgat tcattttacg
     5881 agtcgcgttc cgaaaaaaga ctgttgcggc cagtcggtag agaaaaagcg ctaccatgcc
     5941 gcgttcacat tgatatgtat tcacagggag agaatagcaa tggcaataaa gaaaacggat
     6001 aacaccccac aaaacgtgct caaacaaacc acacaaatgc gtatgtgggt catcattggc
     6061 ggcatcagcg cactgatttc cgtgatatgt aaagttttat cttctggcta tcttgaacct
     6121 tttgacttcg gtcaggctgc gttaggcgtt gtctgcctgg gatatgcgat atcgctgtcg
     6181 aaaaaaatca agcaattacg ccattcgaat aaacagtaag ataacagagg gaagtttaac
     6241 ctccctcttg caaatagtta ctgcacgcgc gccagcgcca cggcctgtcc taactgccag
     6301 accgccattg cgtaatgagt gctgtggtta taacgggtaa tagtgtagaa gttcggcaga
     6361 ccataccagt actggtaacc agtgccaaca tccagacgca gcaagctggc ctgctgatga
     6421 ttccccagcg actgctgcgg cgttagtcca gctgcggcaa gttgtgaaat gctgtacctg
     6481 gttttgaagc cattcggtaa gcctggcgcc tgaccgtttg ccatcaccgc cacctgatcg
     6541 cctttcaccc agccgtgcgc tttgaaatag ttcgctacac taccgatggc atcaaccgga
     6601 tcccacaggt tgatatgccc gtcaccgctg aaatccaccg catattgttt gtaagacgat
     6661 ggcataaact gaccgtaacc catcgcacct gcaaaggaac ctttcagatt gagcggatcg
     6721 tcctgctcgt cgcgcgccat cagcaggaag gtttccagct cacctgaaaa atattccgcg
     6781 cggcgagggt aattaaatga cagcgttgcc agcgcatcga gaatgcgagt tttccccatc
     6841 acgcgccccc agcgggtttc aacgccgata atcccgacga taatttccgg cggtacacca
     6901 tacacctgcc acgcgcgatt caacgcatct tcatactgat tccagaaaac cacaccgttc
     6961 tgcacgttgt ccggcgtaat aaattttttg cgataacgca gccatgcgcc gttcggacct
     7021 gatggcggtt tcaccgatgt ggttggtgcc tggttatcca tcagccgcag taccgaatcc
     7081 agacgtttcg cctgggagag aatttcctgc aactgctgac gatcgaaacc gtgtttgttc
     7141 accattttgt cgatgaactg ctgggcattc gggttattgg cgaagtcacc gcccatctgc
     7201 atcacattgt gctgcggctc aagcaggaag ccgccagacg gcgttccggt ggtcgtttca
     7261 gtctcagtag gttttggctt gctgctacag gcggcaagca acacaaaaag gggaagcaat
     7321 gttacataac gacgcttgaa catgaggggt ccatttaaca gattcaacca ggggcaagta
     7381 tggtaaagca tcccgccccg cacaaggaag cggtagtcac tgcccgatac ggactttaca
     7441 taactcaact cattctcctc actaccttct ttatacaaac tttcaaaata aaatatttat
     7501 ctttcatttt gcgatcaaaa taacactttt aaatctttca atctgattag attagattgc
     7561 cgtttggtaa taaaacaata aatactgaag gagagaacaa tgatagaaac cattactcat
     7621 ggcgcagagt ggttcatcgg gctgttccag aaaggcggag aagtgtttac cgggatggta
     7681 accggcattc ttccgctgtt aattagcctg ctggttatca tgaacgcatt gattaatttt
     7741 atcggtcagc atcgtattga acgatttgct caacgttgcg ccggtaaccc tgtttcccgt
     7801 tatctgctgc taccgtgcat tggtacgttt gtcttttgta acccgatgac cctaagcctg
     7861 ggtcgcttta tgccggaaaa gtacaaaccc agctattacg cggcggcctc ttatagctgc
     7921 cactcaatga atggcctctt cccccatatc aaccctggcg aactgtttgt ttatctcggc
     7981 attgccagcg gtctgacgac gctgaatctg ccacttggcc cactggcggt gagctatctg
     8041 ctggttggtc tggtcaccaa cttcttccgc ggctgggtaa ccgatctgac caccgccatt
     8101 ttcgagaaaa agatgggcat tcaacttgaa caaaaagttc gtctggcagg agcaacatca
     8161 tgacacgcat tcggatcgaa aaaggaacgg gtggctgggg cggcccgctt gagctggaag
     8221 ccacgccggg caaaaaaatc gtctatatca ccgccggtac ccggcctgcg attgttgaca
     8281 aactggcaca gcttactggc tggcaggcta ttgacggatt taaagaaggt gaacccgcgg
     8341 aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac attacgctgc ggcatctatc
     8401 cgaaacggcg tattcccacc attaatatcc actcgacggg caagtctggc ccgctggcgc
     8461 agtacattgt ggaagatatt tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg
     8521 gtgaggcgac accacaaccc tcttccgtgg gccgtgacta tgacaccagc aagaaaatca
     8581 ccgaacaaag cgatggttta ctggcgaagg tgggaatggg tatgggttct gccgttgccg
     8641 tgttgtttca atctggtcgt gacaccatcg acactgtatt aaaaaccatt ctgccgttta
     8701 tggcgttcgt ctcggcgctt atcggcatca ttatggcttc cggtcttggc gactggattg
     8761 cccacggtct tgctccgctg gcgagccatc cactgggtct ggtcatgctg gcgctcatct
     8821 gctccttccc gctgctttca cctttcctcg gcccaggcgc agttatcgca caggttatcg
     8881 gcgtattgat tggcgtgcag attggtctcg gcaatattcc gccgcatctg gctttaccgg
     8941 cactgtttgc catcaacgcg caggcggcct gcgacttcat cccggtcggt ttgtcgctgg
     9001 cggaagctcg tcaggacacg gttcgcgtcg gtgtcccttc tgttctggtg agccgctttt
     9061 taaccggcgc gccaactgta ctgatcgcct ggtttgtctc cggttttatc tatcaataga
     9121 ggctgaaaca tgaccgttat ttatcagacc accatcaccc gtattggcgc gagtgccact
     9181 gacgccctca gcgaccagat gctcatcacc tttcgtgaag gcgcgccagc ggatctcgaa
     9241 gagtattgct tcattcattg ccacggcgag ttgaaaggtg cactccatcc cggtttgcaa
     9301 tttacgctcg ggcggcatcg ctatccggtg accgccgttg gcagcgtggc ggaagacaac
     9361 cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt taagcgaagc ggaatttccg
     9421 ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg cgccgggatc ggttttgaag
     9481 tttgaatctg ttaaggagta aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa
     9541 accttaggcg cgttcctgtg ccacggtctg gctgccgagg ggtatcgcgt ggcggttgtc
     9601 gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa
     9661 ggaacagcgt acggttttgg tgctgacgcc accagcgagc aaagcgttct ggcgctttct
     9721 cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg tctacagcgc cggaatagca
     9781 aaagcagcct ttatcagcga tttccagctt ggagattttg accgttcgct acaggtgaat
     9841 ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt tgatgattcg cgacgggatt
     9901 cagggtcgca ttattcagat caactcgaaa tccggcaaag tgggcagcaa acacaactct
     9961 ggctacagcg cagcgaaatt tggtggcgtc gggctgactc aatcactggc gctggatctg
    10021 gcggagtacg gcattacggt gcattcgctg atgctcggta acctgctgaa atcgcctatg
    10081 ttccagtcac tgttgccaca atacgcgacc aagctgggta tcaaaccgga gcaagtcgag
    10141 cagtattaca tcgataaagt accgctcaaa cgcggctgcg attatcagga tgtgctgaat
    10201 atgctgctgt tctacgccag tcctaaggcg tcgtactgca ctggtcagtc gatcaatgtc
    10261 accggcggtc aggtgatgtt ctgatcaaca gcggagatct tttaaggatc tccgcgcgac
    10321 taatagaatg cctgatgcgc tacgcttatc aggcctacag gacttccgcc actacatcac
    10381 ggagaagtta tggtatccgc actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg
    10441 gccttaggcg gttggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccaacaa
    10501 gggcgcgttg gcgtgggacg ttccagcggg cgctttaaac cacgggtcgt ggtcgccatc
    10561 gcgctggacg atcagcagcg catcgtcgac accttgttta tgaaaggact gaccgtcttc
    10621 gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg cgggtgattt acagcccgat
    10681 gtgatctttc cccatgatcc actatcacag aatgctctat cattggcgct taaactgaaa
    10741 cgtggataat ttcgttgtga atgttactag cttgcgaagt tatcattttg aaacctaaat
    10801 caggtaatca cgcccatgaa acctcgtcag cgtcaggccg ccattctgga gtatctgcaa
    10861 aagcagggta aatgctcggt tgaagaattg gcgcaatact ttgacaccac aggcacaacc
    10921 attcgcaaag atctggtcat tctggaacat gccggaaccg tcattcgtac ttatggcggc
    10981 gtggtgttga acaaagagga atccgatccg cctatcgatc ataaaacgct tatcaacacc
    11041 cacaagaaag agctgattgc agaagctgcc gttggtttta ttcatgatgg cgactcaata
    11101 attcttgatg ctggcagtac cgtgttgcag atggtgcctc tgctctcacg ttttaataac
    11161 atcacggtga tgaccaacag tctgcacatc gtcaatgcgc tatccgaact ggataacgaa
    11221 caaactatcc tgatgccagg cggaacattt cgtaaaaaat cggcctcatt tcacgggcaa
    11281 ctggcagaga atgccttcga gcatttcacc ttcgataaat tgtttatggg caccgacggc
    11341 atcgatctca atgcgggcgt aaccacgttc aacgaggttt ataccgtcag taaggcgatg
    11401 tgcaatgccg cgcgcgaagt gattttgatg gctgactcat cgaagtttgg tcgtaaaagc
    11461 cccaacgtgg tttgtagtct tgaaagcgtc gataagctga ttaccgacgc aggtatcgat
    11521 ccagcgtttc gtcaggcgct ggaagaaaaa gggatcgacg tgatcataac cggagagagc
    11581 aatgagtgaa gcactactga acgcgggacg tcagacgtta atgctggagt tgcaggaagc
    11641 aagtcattta ccggaacgtc tgggcgatga tttcgttcgc gccgccaata tcatcctgca
    11701 ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg ggccacattg gtaagaaaat
    11761 cgccgcaacg cttgccagca ccggcacacc ggcttttttt gtccatccgg cagaagcact
    11821 gcacggcgat ctggggatga ttgaaagccg cgatgtgatg ctgtttatct cttattccgg
    11881 tggcgcgaag gaactggatc tcattattcc gcgtctggaa gacaaatcta tcgcgctgct
    11941 ggcaatgacc ggaaaaccga cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat
    12001 ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg acctccagca ccgtgaatac
    12061 cctgatgatg ggcgacgcgc tggcgatggc agtcatgcag gcgcgtggtt ttaatgaaga
    12121 agattttgca cgctcccacc cagccggggc actgggcgct cgcttgctga acaaagtgca
    12181 tcatcttatg cgtcgtgacg atgccatccc gcaggtagcg ttaaccgcca gcgtgatgga
    12241 cgcgatgctg gaacttagcc gcaccggttt aggtctggtg gcagtgtgcg atgatcaacg
    12301 actggtgaaa ggcgtcttta ccgacggtga tttacgtcgc tggctggttg gcggcggcgc
    12361 actcaccacg ccagtcaatg aagcgatgac ggtcgacggc accacgttgc aatcgcaaag
    12421 ccgtgccatc gacgccaaag aaatcctgat gaaacgaaaa atcaccgccg caccggtagt
    12481 ggatgaaaac ggcaaactca ccggcgcaat taacctgcag gatttctatc aggccgggat
    12541 tatttaatcc ttcagcccca gacgtttcgc cagccgatgc aggttggcga cgtcggtttc
    12601 cagcatccgc gcacaggcgg cccagttgtg atgattttgc gccagtgcct gacgaatggt
    12661 ttcacgctgg aacgcttctg tcgcttcacg cagattttgc ttaacaacgg gcaccgccgc
    12721 cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac
    12781 ttcatcgccg ctgcgggtgg ctctcgacaa tactaccgcc cgatgaatag catgttccag
    12841 ttcgcgcacg tttcccggaa aattgtagtg ttgcagtaaa tttcgcgctc cggcacttaa
    12901 taccacgcgg gagagcccca gccgcaaacg acactgctcg cagaaatacc ccgccagcag
    12961 aatgacatca tcgccccgct cacgcagcgg cggcactgaa agtggaaaca cgctcaggcg
    13021 gtggaacaag tcagcgcgaa atcgccctgc cagtacctct tcgcgtaagt cacggttagt
    13081 cgccgccagc acgcgcacat cgacccgcaa actgcggtca tcgccaacgc gttgaatatc
    13141 gccatactgc aacaccctca gcagcttggc ctgcaatgcc aacgacaact cgccgatctc
    13201 atcgagaaac agcgtgccgt tatccgccat ttcaaacttc ccgctgcggt tactgatagc
    13261 accagtaaac gcccctttca catgcccgaa caactcactt tccgccacac tttccggcag
    13321 tgcggcacag ttgagataga ccagcggatt caccgcccgt ggcgaggctt catgaatcgc
    13381 tttcgccacc agctccttac cggttcccgt ctcaccgcta attaaaacgt tgagatcgga
    13441 tgccgccaca atctcaatct cttttttcaa ttgcgtcatg cctggggaca aaccaatcat
    13501 ctgcgtctgt ttcaccgctt caaacggcgc ggcatcgcct ggcagcatat tctggctttc
    13561 cagttgttca atcagtaacg cattgcttaa cgctcccgcc gccagcgcag caatcaggcg
    13621 taactcttcg tcgctgaaaa catcgaactg atcgggctgc atcccgtcga gcgtcaaggc
    13681 accgatcagg ttttgcccgg caaacaacgg caaaccaacg caggcgtgaa ccttcagact
    13741 ctcctgccct ggaatcagac cgtcataagg atcgggcaat tcgctgtctg cgggaaagcg
    13801 caccacatca cccgcgcggg caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg
    13861 tctaccgagt acatccttcg ccagcccgtc gatggcaagc ggaataaact gccgcgagtc
    13921 gtaacgtagc aacgcagacg catcgcactc cagcacctga cgcagcgtag tgatcaggcg
    13981 ctgaaaacga tcctgatgac caatcccacg ctgcaattcg atggcgatat tcgccagcac
    14041 atcaacggaa aaactcatct ttgcctcact gtcaatttga ctatagatat tgtcatatcg
    14101 accatttgat tgatagtcat tttgactact cattaatgag cataatttta tttatagagt
    14161 aaaaacaatt agataaaaaa ctggcacgca atctgcaatc agcaagacat ctttttagaa
    14221 cacgctgaat aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtt
    14281 ggtcaacgtg actgggaagt gcgtgatttt cacggtacag aatataaaac gctgcgcggc
    14341 agcagctaca acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac
    14401 cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc
    14461 gattacatcg tgattaatca tgcagaagaa gaccacgccg gagcgctgac cgaactgatg
    14521 gcgcaaattc ccgatacgcc gatctactgt acagccaacg ctatcgactc gataaatggt
    14581 catcaccatc atccggagtg gaattttaat gtagtgaaaa ctggcgacac actggatatc
    14641 ggcaacggca aacaactcat ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg
    14701 atgacttacc tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac
    14761 tgcgacgagc atctgttcaa cgatgaagtg gatcagactg agcttttcga gcagtgccag
    14821 cgttactacg ccaatatcct gacgccgttc agccgcctgg taacgccgaa aattaccgag
    14881 atcctgggct ttaacttgcc agtcgatatg atagcgacct ctcacggcgt ggtatggcgc
    14941 gataacccga cgcaaattgt cgagctgtac ctgaaatggg cggcggatta tcaggaagac
    15001 agaatcacca ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc
    15061 gcccagggga ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga
    15121 agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa aaggcgtact ggtcggcact
    15181 tctacgatga ataatgtgat gatgccgaaa atcgccgggc tggtggagga gatgaccgga
    15241 ttacgcttcc gtaacaaacg cgccagcgct ttcggctctc acggctggag tggcggtgcg
    15301 gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct tagcctgaaa
    15361 gccaaatggc gaccagacca ggacgctctg gagttatgtc gtgaacacgg tcgcgaaatc
    15421 gcccgtcagt gggcgctcgc gccgctgcca cagagcacgg tgaatacggt ggttaaagaa
    15481 gaaacctctg ccgccacgac ggctgacctc ggcccacgga tgcaatgcag cgtctgccag
    15541 tggatttacg atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg
    15601 agtgaagtcc cggataactt cctgtgcccg gaatgctctc tcggcaaaga tgtctttgac
    15661 gaactggcat cggaggcaaa atgagcaacg gcattgtgat cattggttcg ggcttcgccg
    15721 cccgccaact ggtgaaaaat attcgcaaac aggacgcctg tattccatta accctgattg
    15781 ccgccgacag catggatgag tacaacaaac ctgacctcag ccatgttatc agtcaggggc
    15841 aacgtgccga cgaccttacc cgccagacgg caggtgaatt tgccgagcag tttaatctgc
    15901 gcctgtttcc gcacacctgg gtaacggata tcgatgccga agcccatgtg gtgaaaagtc
    15961 agaataatca gtggcaatac gacaagttag tgctggcaac cggtgccagc gcctttgtcc
    16021 cgccagtgcc cgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct
    16081 gtgaaacaca actgcgggat gcccgacgcg tgttgattgt tggcggtggt ttgattggta
    16141 gcgaactggc gatggatttt tgtcgggcag gcaaagcggt cacgctaatc gacaacgctg
    16201 ccagtattct ggcgtcgtta atgccaccgg aagtcagcag ccgcttgcag catcggttga
    16261 cggagatggg cgttcatctg ctgttaaaat ctcagttgca aggactggaa aaaacagatt
    16321 ctggcattct ggcaacgctg gaccgccagc gctgcatcga agtggatgcg gtaattgccg
    16381 ccaccggact gcgcccggaa accgccctgg cacgacgcgc cgggctgacg attaatcgcg
    16441 gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga tatttatgcg ctgggcgatt
    16501 gcgcggaaat taacggtcag gtattgccgt tcctccagcc gattcaactt agcgcgatgg
    16561 tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa actcccggcg atgctggtga
    16621 aaatcaaaac gccggaatta ccgctgcatc tggcaggcga accccagcgt caggatttac
    16681 gctggcaaat taataccgaa cgccagggaa tggtggcgcg cggtgttgac gatgctgacc
    16741 agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa
    16801 cgttgtcgat gtaggcgagc tacagtgccc caaatgtcgg atgcggcgct gacgcgtctt
    16861 atccgaccta cagggaacgc atgtgttgat cacataatac gtttatgtcg cattgcactg
    16921 tcccaaaatg tcggatgcga cgctagcgcg tcttatccga cctgcgggga tgaatatgta
    16981 ggccggataa ggcgttcacg ccgcatccgg caatggtgtc caaatgcaac acgctttatc
    17041 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    17101 acccgccatc gcccgcaggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    17161 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    17221 tgataccacg catcgtggcc tgctcacgca tcaacgcggc ccccccctgc gccagcgcat
    17281 catgaaacgc ccacgcacgt tgattaaccg gagcctgcca gttcagccac tgctgccaga
    17341 aggtggcgag atccagttga ttatccacca gcggcatcgt caccggatgc gtcacgccgt
    17401 ggcacgaagt cgccagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17461 ctggcgcaca gcccagcgcc gccgctacgg catcgaacaa ccgcccacac gatgacgcca
    17521 gtggtgcatt gattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca
    17581 cacttaccgt ttcgggataa ctctgccact ccggcacaaa acgcaagcac tgcgtcagca
    17641 ggtttcgcca cggctgcttt gcggcaagat cgccaccagc aagcgccact gcaggcaagc
    17701 cgcccaggtg ctcgcattca cgatagttca cccgcaggca ctctcccccc cacaaagcgc
    17761 cgttctcccc cataccaata ccgtcgagtg tcaacgcaat gacatctccg ccatccagcg
    17821 gccagtgatg ttctgccaga catgccgctg cgtgagcatg atgatgcagc accgtttgcg
    17881 tcggcagatt catttcgcgc gcccactggc tggagacata gcccggatgc gcgtcatgca
    17941 caacgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    18001 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcgc
    18061 cgcgcaccag gcagaaggta tttttcagat ccgcgccgag acacagcaca ggcggaacat
    18121 ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca
    18181 tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta
    18241 tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagccg
    18301 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18361 gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg ccaggggcga
    18421 tatcatcaca aagctcaggt acatattttt tatccaccag cacaatcggc gcggcgggcg
    18481 tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgaccgcca
    18541 gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta ctgttacgtg
    18601 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18661 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18721 attcaagatg ggggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18781 gcgggtcaca gtactctttg tcacaagccg gacacagcgg aaacgccgcc atcacggtaa
    18841 acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg cgggccacag tgggtacagt
    18901 tgataaacgg ataacgataa cgccgctcgc ccggggtatt catttcggca aggcaagcag
    18961 ggcaagtcgc ggcatcggga actatttgcg tattcatagc gccgcctgtg ctctggcgga
    19021 tagtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    19081 gcgccagcgg cggacaatgc tgatgcaatt gaacaagaaa ggtttccggc tcttccagca
    19141 gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg
    19201 ccagttgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19261 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19321 ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga tactgcccgc
    19381 ttcgtattct acgaatattt ccgggaattc ctttgatacc agaacagttc tgtaagattt
    19441 ttagaacatc agcgccgtgc ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19501 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19561 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagccctgc
    19621 gccgctgttg cgaatcaccg gacgcaccac cacttccatc gcaccatatg ggcaagccac
    19681 aacgcaggtt ttgcaaccaa tacaacgttc ctgcatgaca tgaacaaacc ctttatcacg
    19741 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcagac
    19801 tgtcgccgtg gaaatgttca cacctttaat gacatggatt cgcggtaaaa atgtttccgg
    19861 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19921 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19981 caataacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttactgttat
    20041 ttaaggaaat taaattctgt actacaggaa aaac
//
LOCUS       DACADA010000076        19317 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.077, whole genome shotgun sequence.
ACCESSION   DACADA010000076 DACADA010000000
VERSION     DACADA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.077"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..249
                     /locus_tag="ISB17_002722"
     CDS             <1..249
                     /locus_tag="ISB17_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP1980634.1"
                     /translation="ADGGAYPAIKPDDICTLPLIIPSINIIEKFDELTSNFREMMNNS
                     LIQNEYLTNLRGTLLPKLISGELSLEDLPDLTTDTEAA"
     gene            344..3607
                     /locus_tag="ISB17_002723"
     CDS             344..3607
                     /locus_tag="ISB17_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1980635.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYMNPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERNNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSSLN"
     gene            3822..4160
                     /gene="symE"
                     /locus_tag="ISB17_002724"
     CDS             3822..4160
                     /gene="symE"
                     /locus_tag="ISB17_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAP1980636.1"
                     /translation="MTDTHSIALPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAIEESELMQSLRQVCKLSVRKQRQVQEF
                     IGVISSKTPR"
     gene            complement(4203..4367)
                     /locus_tag="ISB17_002725"
     CDS             complement(4203..4367)
                     /locus_tag="ISB17_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HAP1980637.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            4544..5956
                     /locus_tag="ISB17_002726"
     CDS             4544..5956
                     /locus_tag="ISB17_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP1980638.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCGSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRTNCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(6199..7155)
                     /locus_tag="ISB17_002727"
     CDS             complement(6199..7155)
                     /locus_tag="ISB17_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1980639.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLEYASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPATARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELIRRMPQHRERIMTIAERIHNDGWLLGRERGRKEGKEEGEKSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPWIEY"
     gene            complement(7152..7364)
                     /locus_tag="ISB17_002728"
     CDS             complement(7152..7364)
                     /locus_tag="ISB17_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980640.1"
                     /translation="MRLIKRVRWKGEYLPGTGNVKCEPVSGKMYRLQRNKNSLESTSG
                     NEKLPGENGFVSRYSRAILSTGDGSP"
     gene            7622..8854
                     /gene="mdtM"
                     /locus_tag="ISB17_002729"
     CDS             7622..8854
                     /gene="mdtM"
                     /locus_tag="ISB17_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAP1980641.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATIALSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANTIVARFIK
                     DPTEPRFIWRAVPIQLAGLALLIVGNLLSPHVWQWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            8895..10175
                     /locus_tag="ISB17_002730"
     CDS             8895..10175
                     /locus_tag="ISB17_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAP1980642.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARTDAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHHVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            10291..11442
                     /locus_tag="ISB17_002731"
     CDS             10291..11442
                     /locus_tag="ISB17_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HAP1980643.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLHKTVEERFGHE
                     ISEDALRDAIVLKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            11452..12219
                     /locus_tag="ISB17_002732"
     CDS             11452..12219
                     /locus_tag="ISB17_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980644.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12216..12473
                     /locus_tag="ISB17_002733"
     CDS             12216..12473
                     /locus_tag="ISB17_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAP1980645.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12538..13398
                     /locus_tag="ISB17_002734"
     CDS             12538..13398
                     /locus_tag="ISB17_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HAP1980646.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13466..14644
                     /locus_tag="ISB17_002735"
     CDS             13466..14644
                     /locus_tag="ISB17_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980647.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSMLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14657..15211)
                     /gene="kptA"
                     /locus_tag="ISB17_002736"
     CDS             complement(14657..15211)
                     /gene="kptA"
                     /locus_tag="ISB17_002736"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAP1980648.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKVGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            15461..16144
                     /locus_tag="ISB17_002737"
     CDS             15461..16144
                     /locus_tag="ISB17_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980649.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16141..16602
                     /locus_tag="ISB17_002738"
     CDS             16141..16602
                     /locus_tag="ISB17_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980650.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16615..17787
                     /gene="iadA"
                     /locus_tag="ISB17_002739"
     CDS             16615..17787
                     /gene="iadA"
                     /locus_tag="ISB17_002739"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP1980651.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17852..18763
                     /gene="hypT"
                     /locus_tag="ISB17_002740"
                     /gene_synonym="qseD"
     CDS             17852..18763
                     /gene="hypT"
                     /locus_tag="ISB17_002740"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAP1980652.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(18756..19148)
                     /gene="iraD"
                     /locus_tag="ISB17_002741"
     CDS             complement(18756..19148)
                     /gene="iraD"
                     /locus_tag="ISB17_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAP1980653.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(19145..19228)
                     /locus_tag="ISB17_002742"
     CDS             complement(19145..19228)
                     /locus_tag="ISB17_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAP1980654.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         4622 a         4850 c         4868 g         4977 t
ORIGIN      
        1 gctgatggtg gagcatatcc tgctataaaa cctgatgata tttgtacatt acctctaatt
       61 attccatcaa taaatataat tgagaaattt gatgaattaa caagcaattt cagagagatg
      121 atgaataact cattaattca aaacgaatat ctcaccaacc tccgaggcac cttgctcccg
      181 aaactcatct ccggcgagct atccctggaa gatcttccgg atctcaccac cgatacagaa
      241 gccgcataac gcattttgcc cctgtaaaat caggggcttt ctggtaaggt tttctactga
      301 tacgggaatg cttaccagaa attagccagg gttggagcgc gatatgagtc tctctttcag
      361 tgaagcaaaa ttagaacaag cgatcattga actgttacag gatcagggat atcaacacct
      421 gatcggcgat aatgtcccac gttcgagcct cgatcaggtc attatcgaag acgatctccg
      481 tcattattta gcggcacgct accagcctga tggcattact gaagaagaga ttcagcgact
      541 gatcaaacag ttcaccacgc ttccggcctc cgatctttat gaaagcaaca aaacattttg
      601 tcgctggctg gcaaatggtt ttctgttcaa acgcgacgat cggcaacaaa aagatctcta
      661 tattgaattg ctcgacaccc ggcatctacc tgccgcactg cgccagatat ttgacgccga
      721 agatgtcccg ttgcaacagg cggcggaact cccgccttcc tatatgaatc cgccgcttaa
      781 ccggattaag attgttaacc agcttaaaat ctccggcaaa gataatcaga ctcgtattcc
      841 tgacggcatt ctctatatca atggtctgcc attggtcgtc tttgaattta aaagtgcggt
      901 gcgcgagcag gaagccagta ttggcgatgc ctggaagcag ctctgtaaac gctatcgccg
      961 ggatattccg caactgttta tctacaacgc gctctgcatt attagcgatg gcgtaaataa
     1021 ccggatgggc aacctgtttg cgccctatga atacttttac tcatggcgca aagtcaccgg
     1081 taatgaaaac cgtgaacagg acggaattcc atcattgcac tcaatgattc aggggctgtt
     1141 tcatccggta cgtctgctgg atgtgattaa aaactttatc tgcttcccgg ataaagccaa
     1201 gcacgaagta aaaatttgct gccgatatcc gcagtactat gccgcccgca aactctatta
     1261 cagcatcaag caagcgcgta aacctttcgg tagcggtaaa ggcggcactt actttggcgc
     1321 aacgggctgt ggtaaaagtt acaccatgca atttttaacg cgtcttttga tgaagagcgt
     1381 ggagtttgcc agcccgacca ttgttttgat caccgaccgc accgatctgg acgatcagct
     1441 ttctgcgcaa atgtgcaacg ccaaaaatta cattggcgac gacaccatcg ttcccgttac
     1501 cagccgtgaa gatttgcgta atcaactggc aggacgcaat agtggcggag tctttctgac
     1561 aacgatccat aaattcaccg aagacaccga actcctttct gaacgcaaca atatcatttg
     1621 catctcggac gaagcacatc gtagccaggt taacctcgac cagaaagtca tcgttgataa
     1681 agaaagcgga aaagtgcgca aaacgtatgg ctttgcgaaa tacctgcacg attcactgcc
     1741 aaacgccacc tatgttggct ttaccggcac accgattgac gcgacgctcg atgtcttcgg
     1801 tgaggtgatc gacagttaca ccatgaccga atccgttcag gatgaaatca ctgtacgcat
     1861 cgtgtacgaa ggccgtgcgg ctaaagtgat cctggactcc agcaaactgg aagaagtcga
     1921 aaagtattac gaagagtgcg caaacgcagg caccaatgag tggcaaatcg acgaaagcaa
     1981 aaaagccacc gccaccatga atgcggttct gggtgatgaa gatcggttaa aagccctcgc
     2041 agaagatttt gccaaacatt atgaaaaacg cgtagccgaa ggttccaccg taaaaggcaa
     2101 agccatgttt gtttgtgcca gccgtgaaat tgcctgggat ttctaccgtc agcttaaagc
     2161 tattcgccct gcctggtttg aagtgaagca agcacccgat ggcgtcgtcc tgacggaaca
     2221 ggagcagaaa gagttaccgc cttcagaaat ggtgaagatg gtcatgacgc gcggtaaaga
     2281 tgacgacgag gcgctttatg atttactggg cacaaaagaa tatcgcaaag agctggataa
     2341 gcagttcaaa aacgctaaat cgaatttcaa aattgccatt gttgttgata tgtggctgac
     2401 cggtttcgac gtcccggaac tggataccat ctatattgat aaacccttac aaaaacataa
     2461 tcttatccag actatttctc gcgttaaccg taaactggaa ggcaaaagta aagggttagt
     2521 ggtggactac atcggcatta aaagtcagat gaaccaggca ctggcaatgt attcccgcat
     2581 tgatgccacc aactttgaag atattcagca atcggtaata gaagttaaaa accatctcga
     2641 tttgttgggg caagtctttt acgaatttga cagtcgggat tattttagtg gtgagccaca
     2701 agcgcaatta tcctgcctca accgcgcggc ggaatttgtt ctgcgtaccc agaaagttga
     2761 acgtcgtttt atgggactgg ttaaacgcat gaaagccgcc tacgacgtct gctgcggcag
     2821 cgaagcatta tcacagacag aacgtgacta tattcactat tatcttgctg ttcgttcaat
     2881 tgttttcaaa ctgacgaaag gtgacgcacc ggatgttacc cagatgaatg cacgcgttcg
     2941 tgaaatgatt gcagaagcgt taaaagctga tggcgtagaa gaaatttatt ttcttggcga
     3001 taaaaaagcg gaatccatcg atatttttga cgaagattat ctggcgcgaa ttaacaagat
     3061 caaacttccg gcaacgaaga tccagttatt acaaaaatta ctggaaaaag cgatcagcga
     3121 cttcaagaaa gtgaaccagt tgcaagggat taacttcacc cgccgcttcc aggcgattat
     3181 agatcgttat aatgagcggc gagaagatga tgtactcaac ggtgaagaat tcgatacatt
     3241 cagtcaggaa atgaccgata ttatctatga tattaaaacc gaaatgggca cctgggccga
     3301 tttaggcatt gatattgaag aaaaagcctt tttcgacatt cttgctcata tgcgcgataa
     3361 atatcagttc acctatgacg atgaaaaaat gctgtcactg gcaaaagaga tgaaaagcgt
     3421 ggttgacaac acatctaaat atcctgactg gagtaaacgc gatgatatta aagcgaaact
     3481 gaaagttgaa cttattctgc ttctacacaa gcataagttc ccgccagtag cgaatgatga
     3541 tgtttatatg ggtgtgctgg cacaggcaga gaattttaaa gaacaccata tgagttcttt
     3601 aaattaatta tgaaggccgg atacactttg ttatccggcc attctgaatt aacgttaatt
     3661 tcccttccag atctttccag taatgctttt attattttct atttttagct ggacttttcc
     3721 catatttact gatgatatat ccaggtattt agcgcgatgc ggatgtgttg gcgcacaccc
     3781 gcaccgctca tcacaatcac tatttatagg gaatagcagt tatgactgac acgcattcta
     3841 ttgcactacc gtttgaagca gaagtctccc cggcaaataa ccgtcaatta accgtgagtt
     3901 atgcgagccg ctacccggat tacagccgta ttcccgccat caccctgaaa ggtcagtggc
     3961 tggaaaccgc cggttttgcc accggcacgg cggtagatgt caaagtgatg gaaggctgta
     4021 ttgtcctcac cgcccaacca cccgccatcg aagagagcga actgatgcag tcactgcgcc
     4081 aggtgtgcaa actgtcggtg cgtaaacaaa ggcaggtgca ggagtttatt ggggtgatta
     4141 gtagcaaaac gccacgctaa gtttctctcc ccttccccac acgggaaggg gaattttccc
     4201 cctcactcca catcctccct gcgtaaccca atatccctca accgctcatc actcatctgc
     4261 tgtaacactc gtcgggtctg catttgccgc cgccaacgcc tcaccgcctg ccagagttgc
     4321 acaagtctga taaacggcgt tttggctttg ttttcgtaaa attccatctg ctgcctcctc
     4381 atctttcaga ggcttcatca tccgtcttct ggcagttcac aatacagatg caaatattag
     4441 ttttatttaa catacagaca gattaaaaca gcatctgcat ccggtttttt agcctcatct
     4501 gtaccggttt ttctctctgt atggtccaaa taaggaatac agcatgacgc gttatcaaca
     4561 tctggcgact ctacttgccg agcggattga gcaggggctg tatcgtcacg gggagaaatt
     4621 gccgtcggtg cgcagcttaa gtcaggagca cggcgtcagc atcagcaccg tgcagcaggc
     4681 gtaccagacg ctggaaacga tgaagctcat cactccgcag ccgcgttcgg ggtattttgt
     4741 cgcacaacgt aaagcccagc cgccagtgcc gccgatgacg cgtccggtgc agcgcccggt
     4801 ggaaattacc cagtgggatc aggtactgga tatgctggag gcgcatagcg acagttccat
     4861 tgttccgtta agcaaaagca cgccggatgt cgaaacgccc agcctgaaac cgctgtggcg
     4921 ggagctaagc cgggtggtgc agcataatct gcaaaccgtt ctcggttatg acttgttagc
     4981 cggtcagcga gtgttgcgag agcagattgc ccgcctgatg ctcgacagcg gctcggtagt
     5041 caccgccgat gacatcatca ttaccagcgg ctgccataac tcgatgtcgc tggcgttaat
     5101 ggcggtgtgt aaaccgggcg atattgtcgc ggtcgaatcc ccctgttatt acggttcgat
     5161 gcagatgctg cgtggcatgg gcgtgaaagt gattgaaatc ccaaccgatc cagaaacagg
     5221 catcagcgtt gaagcgctgg aactggcgct ggagcagtgg ccgattaaag gcatcattct
     5281 ggtgccaaac tgtaataatc cgctgggatt tattatgccg gacgcgcgca aacgggccgt
     5341 tctctctctc gctcagcgtc atgatattgt gatttttgaa gatgatgtct acggtgaact
     5401 ggcaacggag tatccgcgcc cgcggaccat tcattcctgg gatatcgacg ggcgagtgct
     5461 gctgtgcggc tcgttcagta aaagtattgc acctggcctg cgcgtgggct gggtcgcacc
     5521 ggggcgctat cacgataaac tgctgcatat gaaatatgcc atcagcagct ttaatgtgcc
     5581 gtccacgcaa atggcggcgg caacgtttgt gctggaaggt cactatcatc gccatatccg
     5641 gcggatgcgg cagatctatc agcgcaatct ggcactttat acctgctgga tacgggaata
     5701 ttttccctgc gaaatctgta ttacgcgccc gaaaggcgga tttttactgt ggatagaatt
     5761 gcctgaacag gtcgatatgg tctgcgtcgc gcggcagctg tgccgcatga aaatccaggt
     5821 ggcggcaggc tcgattttct cggcttccgg caaataccgt aattgtctgc gcaccaactg
     5881 cgctttgccg ctcagcgaaa cctatcgcga agcactgaag caaattggcg aggccgtgta
     5941 tcgggcaatg gaataaattc gacatagtgg cgaagcgcct gatttactct cacgaataga
     6001 gtgtgactcg ttttatcttt gcagggtaat cgccacgttc cagcatggct taggcaaagt
     6061 gcgctttgtt cataccggat gcgatgtgaa cgccttacca ggtctacaaa atctttaaag
     6121 ttaaatatat ttaatgggtt atgtagacct gataagcgta gcgcatcagg ctgctttgcg
     6181 tttgtcatca gtctctgatt agtactcgat ccatggatcg cgcttgcttt caggcaaggg
     6241 ctgatctaat gcctgcattt gctctgccga aagtccggta tatcgttgta tccattcagg
     6301 atccgcccca ttctgcaaca acaatcggag gaggctcttt tccccttctt ctttcccttc
     6361 tttcctcccc ctctcccttc ccaacagcca tccatcatta tgaattcgct ctgcaatcgt
     6421 cattattcgc tccctgtgtt gtggcattcg acggataagt tcgctgataa actcattaaa
     6481 acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcggttatct ggctgtcatt
     6541 agcgcaccct gtaaccagta atactgccag ttgatcaata agccccatta gatcgcgctg
     6601 gcgaatatgc ttttggatca actccagcag ggcgactctg cgatgctgca caatctcgtc
     6661 gtctggcacg acagtgacat ccaccagcgg gaacgctgcg gtataaagct tccgtgcggt
     6721 agccgggtcg gcaaattcat ccagccagca caaagaccag gggtaaggac tacggctacc
     6781 gtgataaaac agcatcggga tgaccagcgg tagcggccgg cgtttatcat gctcgatatg
     6841 gcgctgcatc accgccatgg aatatcgcat caggcgaaag gccatatgga tatcctcgcg
     6901 gctctgatgt tcaatcacca cataaatata gccatctcct tcgcgggtct ttaccgacca
     6961 cagaatatcg gaatgtagcg cccgcaattt ttcatcgaca aagctggcgt attccagttt
     7021 taagctgtcg agatcgcaca gttcacgtaa atctttgggt aagtggatct ccataaaatc
     7081 ccgcgcggtg tcagggtggg tgagaaaaga tttaaataat gcgtcatgcg gcgtgctggt
     7141 cgtgaagttt gtcatggcga tccgtcacct gtagataaga tggcccgact gtagcgacta
     7201 acaaaaccat tctcacccgg caatttctcg ttccccgagg tgctttccag gctatttttg
     7261 ttcctctgca aacgatacat ttttccggaa acaggctcgc attttacatt tcctgtaccc
     7321 ggcagatatt cccctttcca ccgcactctt ttgattaacc gcaaatttta attacactta
     7381 aggtgtatat tttctatgca acccatcaat tcaagaggtg taatgtgctg atgactattt
     7441 gtaatcgtta tacattctga cctggagtca gaaggtattt ctctgtctgt atgttcacag
     7501 gcagtgtggt tgattacatg aaattcagta catttgcccg tctcgcctgc ctttcgtcat
     7561 taccgacggt attgaatttc gttttcccca ttgggattct ccggacaagg agttgtttgt
     7621 tatgccacgt ttttttgccc gccatgccgc cacgctgttt ttcccgatgg cgttgatttt
     7681 gtatgacttt gctgcgtatc tgtcgacgga cctgatccag ccagggatca ttaatgtggt
     7741 ccgtgatttt aatgccgatg tcagtctggc cccggctgcc gtcagtctct atctcgctgg
     7801 aggcatggca ttgcagtggc tgctggggcc gctttccgac aggattggcc gcaggccggt
     7861 gctgattacc ggggcgttaa tttttaccct tgcctgcgcc gcgacaatgt tcaccacgtc
     7921 aatgacacaa tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt ttattgccac
     7981 cgttggttat gtcacggtgc aggaggcgtt cggacagaca aaagggatca agttgatggc
     8041 gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg gcgcagctct
     8101 gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg gttttatctc
     8161 atttgtcggc ttgctgctgg cgatgccaga gacggtgaag cgcggcgcgg ttccgtttag
     8221 cgccaaaagc gtcttgcgcg attttcgcaa tgtcttttgc aaccggctgt tcctctttgg
     8281 cgcggcaacc atcgccctca gctatatccc aatgatgagc tgggtggctg tctcgccggt
     8341 gatcctgatc gatgcgggcg gcttaacaac ttcgcagttc gcctggacgc aggttccggt
     8401 attcggcgcg gtgattgtcg ccaacaccat cgtggcgcga tttatcaaag atccgaccga
     8461 accgcggttt atctggcgtg ccgtgcccat tcaactggcc ggcctcgcac tgttgattgt
     8521 cggcaatttg ctgtcgccac acgtctggca gtggtcggtg ctcggcacca gtctgtatgc
     8581 tttcgggatt ggtttaatct tcccgacctt attccgcttt acgctgtttt ccaataactt
     8641 accgaaaggg accgtctccg catcactgaa tatggtgatc ctgatggtga tgtctgtctc
     8701 ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc
     8761 cgtcgtagcg ggcgttatcg tcgttttcac cctggcggga ttgctcaatc gcgtgcgcca
     8821 gcatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct
     8881 ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg ttggcgctct
     8941 ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgcca cccaattttt
     9001 gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg gcggactggt
     9061 ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt catacggcga
     9121 ttatcccgcg taataaagac cggattggcg aaaatctcgg tcagttcgtg caggaaaaat
     9181 ttctcgatac ccaatcgctg gtggcattga ttcgacgtca cgaaccggcg ttgttgattg
     9241 gcaactggtt tagtcagcca gaaaacgccc gccgcgttgg tcagcatctg ttgcagatca
     9301 tgagtggttt tcttgaactg accgatgatg cgcgtattca gcgcctgctt aagcgcgcag
     9361 tccatcgggc gattgataag gtcgatcttt ccggcaccag tgcgttgatg ctggagagta
     9421 tgaccaaaaa cgatcgtcat caggtgctgc tggatacgct gatcgcacag ttgatcgccc
     9481 ttctccagcg cgataaatcg cgcaagttta tcgcccagca gattgttcgc tggctggaga
     9541 gtgagcatcc actgaaagcc aaaattttgc ccactgaatg gctgggcgaa catagcgcgg
     9601 agttggtttc tgacgcggtg aattctttgc ttgatgatat tagtcgcgat cgtgcgcatc
     9661 agatccgcca tgcgtttgat cgcgccacct ttgccctgat cgacaagctg aaaaacgatc
     9721 ctgaaatggc agcgcgaacc gatgccgtaa aaagctatct gaaagaagat gaagctttta
     9781 accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg gatatcaaca
     9841 gtgaagattc tcgtgtgaaa gaacgcatcg cacgagcggg tcaatggttt ggcgaaacgt
     9901 taattgccga tgatgccttg cgggcgtcgt taaatggtca tcttgaacaa gccgcgcacc
     9961 acgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgatacg gtaaaaagct
    10021 gggatgcgcg ggatatgtcg cggcaaatag aattaaatat cggcaaagat ctgcagttta
    10081 ttcgtgttaa cggtacgctg gttggcggat gtattgggct gattttgtat ttgctgtcgc
    10141 agctcccggc tttgttcccc ctcggcaatt tttagaaatc catgaaaaac taagtggcta
    10201 accgcgagag agatcaaata aaccgcacta aaagaatggt aataatgtgg cttcttttca
    10261 ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct acccgccatt
    10321 ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga tctcaaggag
    10381 cgcggcattc cgctggttgg tacctactgt acttttatgc cgcaagagat cccaatggca
    10441 gccggtgcgg ttgtggtttc actctgttcc acctctgatg aaaccattga agaagcggaa
    10501 aaagacctgc cgcgcaatct ctgcccgctg attaaaagca gctacggctt cggcaaaacc
    10561 gataaatgcc cctacttcta cttttctgat ctggtggtcg gtgaaaccac ctgcgacggc
    10621 aaaaagaaaa tgtatgaata catggcggaa tttaagcccg ttcatgtgat gcaactgcca
    10681 aacagcgtta aggacgatgc ctcacgcgcg ttatggaaag ccgagatgct gcgcttgcat
    10741 aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctacg cgatgccatt
    10801 gtgctgaaaa accgcgaacg tcgcgcactg gccaactttt atcatttggg gcagttaaat
    10861 cctccggcgc ttagcggcag cgacattctg aaagtagtct acggcgcaac cttccggttc
    10921 gataaagagg cgttgatcaa tgaactggat gcaatgaccg cccgcgttcg tcagcagtgg
    10981 gaagaaggcc agcgactgga cccgcgtccg cgcattttaa tcaccggctg cccaattggc
    11041 ggcgcagcag agaaagtggt gcgcgcaatt gaagagaatg gcggctgggt tgtcggttat
    11101 gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg cagaaacggg cgatgtctac
    11161 gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag
    11221 cgcctgcaaa tgctcagcca gatggttgaa gaatatcagg tcgatggcgt agttgatgtg
    11281 attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt
    11341 catcagcaca acattcctta tatcgctatt gaaacagact actccacctc agatatcggg
    11401 cagctcagca cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtggcatat
    11461 tcgattggca ttgattccgg ctcaaccgcc accaaaggga tcttactggc agacggcgtg
    11521 attacgcgcc gtttcctcgt tccaacaccc tttcgcccgg caacagcaat tactgaagcc
    11581 tgggaaactc tgcgcgaagg gttagaaaca acgccgtttc tgacgctcac cggctacgga
    11641 cggcaactgg tggattttgc cgataaacag gtgacggaaa tctcctgtca cgggctgggc
    11701 gcacggtttc ttgcgccagc aacgcgcgca gtaatcgaca tcggtggtca ggacagcaaa
    11761 gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg
    11821 gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa
    11881 ctcgacagca ttaccgaaaa tgtcataccg cacgccatca cgagtatgtg cacagtgttt
    11941 gctgaatcag aagtgatcag cctgcgctct gcgggcgtag cgccagaagc gattctcgcc
    12001 ggagtgatta acgcgatggc gcggcggagc gccaatttca ttgctcgtct ctcctgtgaa
    12061 gcgccgattc tgtttactgg tggcgttagc cactgtcaaa ggtttaccca catgctggaa
    12121 agccatctgg gaatgccggt acaaactcac cctgatgcgc aattcgctgg tgcaattggc
    12181 gcggcggtga tcggtcagcg gcagagaaaa cgcgcatgaa agagtatctg tttttatttc
    12241 actccacggt cggcgtcata caaacccgca aggcgttgca ggcagcgggc atgacttttc
    12301 gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct
    12361 gtccgcctgg cgaggaaatt caatgggtga tccctgggca tacggaatca gtttattgtc
    12421 agcaggatgg tggctggcgc tgcattgcac attacgggat ttctccgcgt taaggattca
    12481 ttaagatcgc tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg
    12541 acaaaaagta tttcgttaag taaacgcatc ttcgttattg tgattctttt tgtcattgtg
    12601 gcagtgtgta cattcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt
    12661 cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct
    12721 tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc
    12781 attgttgaaa tgaccaccaa tggcgatttg atccgcacaa ttcctctgga ttttgttaag
    12841 gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat
    12901 tatgccattt atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata
    12961 aaaatcccgc tgcaggaatc cccaaccaat tgcggttttg aaggattggc ctattctcgc
    13021 caggatcaca ctttctggtt ttttaaagag aaaaacccga ttgaggtcta caaagttaac
    13081 gggctgttaa gtagcaatga attgcacatc agcaaagaca aggcactaca acggcaattt
    13141 actttagatg atgtgtccgg cgcagaattt aaccaacaaa aaaatacgct tctggtgctg
    13201 tctcatgaat ctcgcgcact ccaggaagta acgctggctg gcgaggttat aggtgaaatg
    13261 tcactaacca aaggcagtcg aggtttgtcg cataacatca aacaggccga aggggttgcg
    13321 atggatgcct caggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc
    13381 cctcaatcat cgcattaaca ggctggcatg tttcaccttt ttgttattaa cttgttaaca
    13441 acaaaaatga caaacaaggt taaacatgcc ttcgccctcg catcccgtag aacgcttttc
    13501 tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggtt taggccgctt
    13561 cctttatacg cctatgttgc ccgtcatgat ggcggaagga tcgttttcat ttagccagct
    13621 ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcgtt
    13681 tggcgcattt caccagccat cgcgcctgcg cccatttctg ttggcttctg ccctggcgag
    13741 cgggttgttg atcctcgcaa tggcatggtt gctgccattt attctggtgt tattgattcg
    13801 cgtcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgatgc tgattatgca
    13861 gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca ttggcatcgc
    13921 actgggcaat gaatatgttc tggcaggcct gcattttgac ctctcctcgc aaacgttatg
    13981 gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc
    14041 gaaaaaacac gccatcacac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
    14101 gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg tcgcgactta
    14161 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
    14221 agtcggctta tcaattgtgc ctggttgctt tggctggcta tgggcagcaa aacgttgggg
    14281 ggctttgccc tgcctgacgg cgaatttgct ggtgcaggct atctgtgtgc tgcttactct
    14341 cgccagcgac tcgcctctct tgcttatcat cagcagtctt ggttttggcg gtacctttat
    14401 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgtaccgg gaaatctcaa
    14461 tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac
    14521 cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc
    14581 gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc
    14641 atgaggagtg tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga
    14701 ccccgttttc cgcttgccag aaagggaggc ctcgtttcgc catttcctgt gctttgacgg
    14761 ttaaaataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
    14821 agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc tcgtcaagga
    14881 aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag
    14941 cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
    15001 taaaacgctt tttatcgctg gtggcaacca cggtatccag caatgcacgg gttaaccgct
    15061 tgcccacttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca
    15121 gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
    15181 ccgccagctc tttttcgttg tactttgcca tcgcgttttc gtccctggat tatctgtttt
    15241 ttatttacta tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgtaga
    15301 accttttcca cacagcgtaa ttttaccgct atgcaaaaaa cgcatagtca gaaacatact
    15361 tgcattttcc ccatacccac atggctgata aggtttttca gctccaggaa ttttcctggc
    15421 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
    15481 tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
    15541 caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc gcgtttttga
    15601 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
    15661 gtttcgcggc gcgggtggcg cgggggctaa agatggcttt ctctttgcgc tggagctggc
    15721 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gcggattacg
    15781 cgccgcgcag cagttaatga cgccagtgct aaaaccgttg ttgggcattc cagggatctg
    15841 ctcgctggcg ctgatcgcta acctgcaaaa caccgatgcc gccgcaggta tgaccaaaga
    15901 gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
    15961 cagcggtagc gccattatca ccaactactt ctcttccggc gtcgcggtgt ttgcctttct
    16021 gggcacgtcc gtaatcgtac ctttagcagt catcctggtg tttaaatttg tcggagccaa
    16081 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
    16141 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggtgc gcgccgtggc
    16201 tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag
    16261 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
    16321 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggt
    16381 ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc
    16441 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
    16501 ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt
    16561 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
    16621 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
    16681 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagtaatatc
    16741 ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat cctctgccca
    16801 ggttttattg atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg
    16861 cgcacgccgg aagtggcgtt aagtcgtctg acggaagcag gcgtcacgtc agtggttggt
    16921 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
    16981 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
    17041 actattacgg gttccgtgga aaaagacgtg gcgattatcg atcgcgtgat tggcgtgaaa
    17101 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
    17161 gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg
    17221 ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg cgatgtgccg
    17281 attagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg
    17341 gagttcgcgc gcaaaggcgg caccatcgat atcaccagca gcattgacga accagtcgcc
    17401 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    17461 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    17521 ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agattatgat
    17581 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    17641 agcgggaaag gcgaaattct gccaggcaat gacgcagact tactggtcat gacgccagaa
    17701 ttacgcattg agcaggtata cgctcgcggc aagctgatgg tcaaagacgg caaagcctgc
    17761 gtgaaaggaa cgtttgaaac ggcataaacg gttgtcagcc gcccgaatgg acgtagaatg
    17821 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcgg ttttgcataa
    17881 tattgaaact aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca
    17941 ggcagcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga
    18001 acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcggaaca
    18061 aggtaaaata ttccattcgc agatccgcca tctgttgcaa cagttagaga gcaaccttgc
    18121 agaactccgt ggtggcagcg attacgcgca acgcaaaatt aaaatagccg ctgcacactc
    18181 tctttccctc gggctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    18241 aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc aaagtgactg
    18301 tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca ttcgcttatt
    18361 tgaatctcaa ttgttccccg tttgcgccag tgacgaacac ggagaagcac tttttaacct
    18421 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    18481 taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg tctcttcgat
    18541 gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc taccggaata
    18601 cgccatccaa caagaaattc gcagcgggca gctcgttgta ctgaatcggg acgaactggt
    18661 gatcccgatt caggcctatg cataccggat gaatacccgc atgaatccag ttgccgaacg
    18721 cttctggcgt gaactgcgcg agctggagat tgtgcttagc tgacattctc cagcgtcgca
    18781 ctgcgccaac cgccattatc ccagaccaga tcaaacgtta gcgattcatc gtcgtgatac
    18841 agcattatct caaggctgat cgccagagtg tggcagtacg cttctttcaa attaacctgt
    18901 aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag
    18961 ctctggagat gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt
    19021 aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct gcatactgat
    19081 ttacgcagcg gtgacgtttt attgcctgaa atttcaggta aaacagcctg aagtgattgg
    19141 cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg
    19201 caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc
    19261 agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaa
//
LOCUS       DACADA010000077        19205 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.078, whole genome shotgun sequence.
ACCESSION   DACADA010000077 DACADA010000000
VERSION     DACADA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.078"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            162..554
                     /locus_tag="ISB17_002743"
                     /pseudo
     CDS             162..554
                     /locus_tag="ISB17_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            607..1002
                     /locus_tag="ISB17_002744"
     CDS             607..1002
                     /locus_tag="ISB17_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980655.1"
                     /translation="MENMMKTMREHFPTRTVVIEQSLDMFGVKRNEDGTLLFPRHSDK
                     RFPIARIRKRLAAYGWRGERLNKEVARIVAGPDIYAERNEYNIIFPYGWEKKQLTREM
                     RRMFPRKTGRRVLIMLDELHHFSDLREKP"
     gene            999..1610
                     /locus_tag="ISB17_002745"
     CDS             999..1610
                     /locus_tag="ISB17_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="HAP1980656.1"
                     /translation="MTLTEKSGHLAWCALVALALARQEGGVLSPAQENLFLIRWLATA
                     LKQRRFPREINPDIEWLLKQRRQLGVSAKLASKLNYLWRSCTGELSEQNDLFRLTYAL
                     ETAKDMHWNYRLLSDREWSGRNAVALSAGVNGIYLSRANLDVAFDDSGRQINPLTARL
                     TGNVVGVMKVFNRCGWQAEPESGASLPHQYSLMAGQGVPGKGD"
     gene            complement(1607..2557)
                     /locus_tag="ISB17_002746"
     CDS             complement(1607..2557)
                     /locus_tag="ISB17_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1980657.1"
                     /translation="MKKKPGTPTPHDATFRQFLANPDVARDFMMLHLPAELRALCDLT
                     TLKLESGTFVEDDLRQYVSDILWSMKTTTGDDGYIHLLLEHQSSADKNMAFRQLRYAV
                     AAMQRHLEAGHKKLPLVIPVLFYHGSRSPYPFSTNWLDCFSNPEQAGKVYTTGFPLVD
                     VTVIDDDEIMNHRRMAALTLLMKHIRQRDMMELLDRLPRVMVEWISPEQVRVLINYMV
                     NAGEAPAPEFMRALAERLPQHEDELMTIAQQLEQIGIEKGLQQGLQQGLQLGEQRGIE
                     KGEREATLKIARTMLQNGIDRNTVMKMTGLTEDELAQIRH"
     gene            complement(2704..2904)
                     /locus_tag="ISB17_002747"
     CDS             complement(2704..2904)
                     /locus_tag="ISB17_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980658.1"
                     /translation="MAERISQREQELLEEFLCTVLDDFSKGSITHHQAVSGIATLYTA
                     ATEGRREEALEYLREGRKLLRQ"
     gene            complement(2958..3590)
                     /locus_tag="ISB17_002748"
     CDS             complement(2958..3590)
                     /locus_tag="ISB17_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1980659.1"
                     /translation="MFVFGYLRASTSEQNASRAKNALKAFANQNGHRIAGWYIDNVSG
                     TTMNRPELIRLLGDAEPGDVILIEQVDRLTRLDDAGWETLRKQITDKQLAIVSLDLPT
                     SHLALSSTVSDDFTRSMLKAVNGMMLDMLAAIARKDYEDRRRRQAEGICKAKTEGKYR
                     GRVADAQKHELIRTLRLAHGKSLRETARLAGVSKMTVIRVCNGNHKQDTD"
     gene            3953..5158
                     /locus_tag="ISB17_002749"
     CDS             3953..5158
                     /locus_tag="ISB17_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP1980660.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTDYHQTYTR
                     NAVAKLPKLSRRIVELAMKEMEEDGYIFNKKQIGNVEQYALTIKNVIDIYAHRQIPKY
                     RDIHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNDLDVETLREAVIRPTIIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGLKPSEVLRKTIIDRIAGDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEDEGVEPRLSASIGFMSKMTNKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGESK"
     gene            5155..6126
                     /locus_tag="ISB17_002750"
     CDS             5155..6126
                     /locus_tag="ISB17_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1980661.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGDVKTFTLKSGKQAKFVKT
                     VVLSGEIESKTFVDASVNGRDQAMLTRESVSDISRTIKLQQFFPAIGREVNGRIEILD
                     GTRRRAACIFNNVKFEILVTKDDISLADARQLAQDIQTAREHSLRELGKRLEVTYGTS
                     MTKEEIALKENLSPAKVTRAFQAAAVPDEMVAVFPVINDISLSDYQFLLKLAEEANNK
                     QTSVTELMEKVQHRLKTMPDYPAIDKSKILAAIRVESKSLTTRPTRTVQTEKLREFSD
                     RNQFARKKTDSKKRLVVYEFSRISAEAQSEIDDAIKRILKRLPESSE"
     gene            complement(6262..7533)
                     /locus_tag="ISB17_002751"
     CDS             complement(6262..7533)
                     /locus_tag="ISB17_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1980662.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRNRPVVVLSNNDGCVIARSAEA
                     KRLGIKMGVPWFQLKTAQFPEPVITFSCNYELYASMSNRVMSHLEELAPRVEQYSIDE
                     MFLDIRGIDSCINYEDFGRQLREHVRSGTGLTIGVGMGPTKTLAKSAQWASKEWRQFG
                     GVLALTSGNPKRTDKLLSRQPVEEIWGVGRRISKTLNTMGITTALQLARANPVFIRKN
                     FSVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGERVTTYEAMRQAVCQYAERAA
                     EKLRGERQFCRHIAVFVKTSPFAVNEPYYGNVASEKLMTPTQDTRDIIAAAVKALDRI
                     WVNGHRYAKAGCMLNDFTPTGVSQLNLFDDTQPRSNSNQLMKVMDGINHSGLGKVWFA
                     GRGIAPEWQMKREMLSPAYTTRWSELPVAKL"
     gene            complement(7533..7955)
                     /gene="umuD"
                     /locus_tag="ISB17_002752"
     CDS             complement(7533..7955)
                     /gene="umuD"
                     /locus_tag="ISB17_002752"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1980663.1"
                     /translation="MILFVTPASEPALSTAPLFTERCPAGFPSPAADYTEDELDLNAY
                     CIRRPSATYFVRAIGDSMKDMGLHSGDLMVVDKAEKPMQGDIVIAETDGEFTVKRLQL
                     KPRIALLPMNPAYPTLYPEELQIFGVVMAFIHKTRGTN"
     gene            complement(8135..8806)
                     /locus_tag="ISB17_002753"
     CDS             complement(8135..8806)
                     /locus_tag="ISB17_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAP1980664.1"
                     /translation="MCGRFTQYNSREEYLAFLGEEAERDIPYDPEPVGRYNVAPGTRV
                     LLLSERRSQLHLDPVHWGYAPEWWNKPALINARSETAAGSRMFRPLWEHGRAIVFADG
                     WFEWKQQNGVKQPFFIYRKDGNPLLLAAIGKPPFENGNDQEGFLIVTAAADEELLDIH
                     DRRPLVYSPDAARKWLSENTTGKEAEDIAREGSLPAEDFTWHPVSRAVGNPGNQGREL
                     IEPLT"
     gene            9165..9842
                     /locus_tag="ISB17_002754"
     CDS             9165..9842
                     /locus_tag="ISB17_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1980665.1"
                     /translation="MSRFIQGNCVHIMSGFPDNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACHEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPPLPQNPLNDVIAWKYSGNRHHPTEKPVTSLQPLIESF
                     THPGAIVLDPFAGSGSTCVAALQAGRRYIGIELLEQYHRAGQQRLAAVRRAMQYPAAN
                     DEFPEAA"
     gene            9842..10063
                     /locus_tag="ISB17_002755"
     CDS             9842..10063
                     /locus_tag="ISB17_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980666.1"
                     /translation="MNYAGHEKLRAEVAEVANSMCDLRATLNGMEHRYRFDSDVLAER
                     LTRQTLFRINALFMAAYNEMLELDACFKD"
     gene            10074..10493
                     /locus_tag="ISB17_002756"
     CDS             10074..10493
                     /locus_tag="ISB17_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1980667.1"
                     /translation="MYGTCETLCRLLREQYPAETPLNLIVWSPADIEALADGMEYAVS
                     EQDTRAVLARMDAIPEEQRLESGVSAGAVMDLIEQVKEAVPAVMVPADLLETLLTTAE
                     QALWHREWTARDCNHPVPESVTRRLADAAKVRALLKK"
     gene            10547..11326
                     /locus_tag="ISB17_002757"
     CDS             10547..11326
                     /locus_tag="ISB17_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980668.1"
                     /translation="MQETTTLNALVMRRARDLIADYGWPDHTDVDQRDPVNKPGWISI
                     YVRLDAANIVHLLPLLCTGDVPADLQSAMTKISGTPAQIILSGSRYADAPQLPEDGTQ
                     IAFPWAGEWLTEPEIQAVTDCLSRAVRDVSRQVWEDARRIKAALNTRGETLFYRQTRN
                     FRLVVKENDMPCWLDDDDNLPVVLDAILNKGARYSSVEFFVISDKVDQILACGQMCDV
                     LRIPGEPPRRWMDLTLLHEVMAEARAEISLVRNALSAIRPV"
     gene            11731..12237
                     /locus_tag="ISB17_002758"
     CDS             11731..12237
                     /locus_tag="ISB17_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP1980669.1"
                     /translation="MRTTTTTPAVYVGTYHKYNCGSIFGKWFDLTEFDGREDFYEACQ
                     ALHADEWDAEFMFQDWEGIPSQFVSECSIDWDFIAAYKRAEEEGREAAFIAWAEYTGE
                     CDYDAFDDAYRGEAESEEDYAREMVEDNGLLNEVPEPLRSYFDFEAWARDLFSSGYVF
                     HDGYVFSN"
     gene            12280..12471
                     /locus_tag="ISB17_002759"
     CDS             12280..12471
                     /locus_tag="ISB17_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980670.1"
                     /translation="MKLAPETREILRQYKALINARRRDAGQRELTTAQVMDEICEYMT
                     CQCAVYIGGHFILQGGKGQ"
     gene            12664..12918
                     /locus_tag="ISB17_002760"
     CDS             12664..12918
                     /locus_tag="ISB17_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1980671.1"
                     /translation="MSKWNIASFSKEDQDKVSVDKVAAAVAWQERMNRPVVPELAERE
                     QPEHLRQYFRERLDVHRQNSQQLPRANAPEYHKPGDEQQK"
     gene            12953..13270
                     /locus_tag="ISB17_002761"
                     /pseudo
     CDS             12953..13270
                     /locus_tag="ISB17_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13446..13658
                     /locus_tag="ISB17_002762"
     CDS             13446..13658
                     /locus_tag="ISB17_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980672.1"
                     /translation="MTQHQGLQHPYQDAPPLSRRGLRSLHPGENQRPPGCKGQRRFAP
                     ALSVVAAGSCSFLAWFFHPFESFRRG"
     gene            complement(13676..13825)
                     /locus_tag="ISB17_002763"
     CDS             complement(13676..13825)
                     /locus_tag="ISB17_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980673.1"
                     /translation="MTFCGDLLNAHAGCYLHPFESRSPQGRKPARAMADVRGAVYRVP
                     FTLDS"
     gene            13896..14126
                     /locus_tag="ISB17_002764"
     CDS             13896..14126
                     /locus_tag="ISB17_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1980674.1"
                     /translation="MSEALAVLPDDTFTREQAEVVAAQYTNVAIEDDQGTHFRLVVRQ
                     NGVMVWRAWNFEADTGKWLNRYIERYGIRKQQ"
     gene            14197..16206
                     /locus_tag="ISB17_002765"
                     /pseudo
     CDS             14197..16206
                     /locus_tag="ISB17_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211744.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            16249..16680
                     /gene="psiB"
                     /locus_tag="ISB17_002766"
     CDS             16249..16680
                     /gene="psiB"
                     /locus_tag="ISB17_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1980675.1"
                     /translation="MRTILTPDVLKTMSCDEFEDWRDSGEDFRRELTHAVMRDLSCPE
                     NWDVNGEYRSEFGGFFPVQIRFTPSHGNYHIAVCSPGAISPAWMVVFVPASGRPFSVI
                     RTLSGYQPELVSHTVSLTARLDADGYSQASIISILAAEGAA"
     gene            16677..17405
                     /locus_tag="ISB17_002767"
     CDS             16677..17405
                     /locus_tag="ISB17_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1980676.1"
                     /translation="MIPANLSLVPFSPERRAAMEAIAEVERRRSRGAVSTMYPYASAF
                     FRRLTGSTRITIREIHHFAPVLTARELRGSRDSWLSAIDALIESRGTRCWLPLPVGAG
                     QRLFPEVAFQQTERVRCQDALRDEKYTRQRHKERCQREQAYQALAGQAEIELAFHTPE
                     TVSSWSARWSGTELRQYDLEEMFWRWSERFPSLEPMERWTMESQPFWTVMAETNALAR
                     ESPGSVRQLERWMVPNKLTARSQV"
     gene            17787..18146
                     /locus_tag="ISB17_002768"
     CDS             17787..18146
                     /locus_tag="ISB17_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980677.1"
                     /translation="MRYWILVALLNGLFTLVIHYAAPEMLGGTAYYAGVAICALIALV
                     ITWRDPPGMLDNVLLFLLKWGIVASGLAFFIWGIGYKLEFWGDHNPDEIGGAERCSAS
                     GQWLGVLEAKVTNYTPA"
     gene            18772..19146
                     /locus_tag="ISB17_002769"
     CDS             18772..19146
                     /locus_tag="ISB17_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980678.1"
                     /translation="MNTQNVNVKTATKESSERWVKTSSLRTDGFVRNIHSRNPFDVIR
                     ADVVLARMEIQANRGCGLHYEIYESRLLGMAMHYLAELPLKDRPAFMGAAAKRGYMLT
                     TAEEERADGECADLMIELAADY"
BASE COUNT         4215 a         5116 c         5403 g         4471 t
ORIGIN      
        1 aatctgctcc atgcgggaga ggaaaatctc ttttcgggtc tgacggcgct tagtgctgaa
       61 ttcactatcg gcgaaggtga gttgatggct catgatgtcc ctctgggatg cgctccggat
      121 gaatatgatg atctcatatc aggaacttgt tcgcaccttc catcagtgac gatgatctgg
      181 tcagtgcgcc atcgtggccc gatattgcgc agcagctgca gcaccatatc ggccggcgcc
      241 cgctggttat tttcaatgct gagtttgata cgcgcattct gaagcagaca gcggcagcgc
      301 ataacgaccg tgccagctgg ctggactctc tgacggtgta ctgcgccatg cgacttgcag
      361 cggggtatta cggccccacc aatcgctacg gtaccatatc gctttccggc gctgtcagtc
      421 aggctgggct gagctgggcc ggggaggcac actccgccgt cactgacgca gttatgacgg
      481 ctcgggtggt aaacaatatt gccggatact ggcgtgagat ccagtgtgaa atgaatgatg
      541 gcgcagggag ataacgctgc tctctggctg gtcagacagc tgcaggtatt aattcaggag
      601 tctggaatgg aaaatatgat gaaaactatg cgtgagcatt ttcccacgag aacagtggtg
      661 atcgagcagt ctctggatat gtttggcgtc aaacgaaatg aggacgggac gttactcttc
      721 ccccgtcact ccgataaacg ttttcccatt gcacgtatcc gtaagcgact ggcggcttat
      781 ggctggcgag gcgaacgtct gaataaggag gtagcgcgta tcgtcgcggg ccctgatatt
      841 tacgctgaga gaaatgaata caacattatt ttcccttacg gatgggagaa gaagcagctg
      901 acgcgtgaga tgcggcgaat gtttccccgt aagacgggcc gacgtgtgct gattatgctg
      961 gatgaattac atcatttttc tgacttgcgg gagaagccgt gacgctgaca gaaaaatcgg
     1021 gccatctggc atggtgtgcc ctggtggcgc ttgcgctggc caggcaggag ggtggcgttc
     1081 tctcgccggc acaggaaaat ctctttctca tccgctggct ggcgactgcg ctgaagcaac
     1141 ggcgtttccc ccgggagata aatccggaca tcgagtggct gttgaaacag aggcgccagc
     1201 tgggcgtcag cgcgaagctg gccagcaagc tgaattacct ctggcgttcc tgcaccggcg
     1261 agttgtccga gcagaacgac ctgttccggc tgacgtatgc tctggaaaca gcgaaagata
     1321 tgcactggaa ctaccgtctg ctgagcgatc gggaatggtc aggccgaaat gctgtggcgc
     1381 tcagtgcggg cgtgaacggc atttatctct cacgggccaa cctcgatgtc gcttttgacg
     1441 acagcggccg acagataaat ccgcttacag cacgtctgac gggtaacgta gtgggggtga
     1501 tgaaggtgtt taatcgctgt ggctggcagg cagagcctga gtctggtgcc tccctgcccc
     1561 accagtattc tctgatggcc ggacagggag tgccaggaaa gggggattag tggcggatct
     1621 gcgctagttc gtcttcagtc agaccggtca ttttcatgac ggtattgcgg tcaattccgt
     1681 tctgcagcat ggttcgtgca attttaaggg tggcttcgcg ctcgcctttt tcaataccgc
     1741 gttgttcacc taactgcagg ccctgctgca gaccctgctg caggcccttc tcgatgccaa
     1801 tctgttcaag ctgttgtgcg atggtcatca gttcatcctc gtgttgcggc agacgctctg
     1861 ccagcgcccg cataaattca ggtgcaggcg cttcacctgc atttaccatg tagtttatca
     1921 gaacgcgcac ctgttcgggg gaaatccatt ccaccattac ccggggtagc ctgtccagca
     1981 gttccatcat gtcccgctgg cggatatgtt tcatcagcag ggtcagtgcg gccatccgtc
     2041 tgtggttcat tatctcatca tcatcgatga cggtgacatc aaccagcggg aatcctgtcg
     2101 tgtatacttt tccggcctgt tccgggttac tgaaacaatc cagccagttc gtggagaacg
     2161 ggtacggact ccggctgccg tggtaaaaca gaaccgggat gaccagcggc agttttttat
     2221 gacccgcttc aagatgacgt tgcatggcgg cgacagcata tctgagctga cgaaaggcca
     2281 tatttttatc cgccgaactc tggtgttcca gaagcagatg gatgtacccg tcatcgccgg
     2341 tggtggtttt catgctccag agaatgtcgc tgacgtactg acgtaagtcg tcctcaacga
     2401 aggtaccgga ctccagcttc agcgtggtca aatcgcacag cgcgcgtaat tcagccggga
     2461 gatgtaacat catgaagtca cgggcgacat cgggattcgc caggaactgg cgaaatgtcg
     2521 cgtcatgggg tgtgggtgta cctggtttct tcttcatcag tcctgggctc tgaaaaatga
     2581 cccggagatg ttatcacagc ccgggaaaag ctaatattgg cggtgaacgg aattgccatt
     2641 ccttcagggg accgttccgg ggtggttcgg tccccttcct aaaccatctt tactcatcat
     2701 caattactgc cttagcaact ttcttccctc tctcagatat tctaaagcct cttctctccg
     2761 tccttctgtg gcagctgtat ataatgtcgc gataccgctg acagcctgat ggtgggttat
     2821 actgccttta ctgaaatcat ccagaacagt acaaaggaat tcctccaaca actcctgttc
     2881 tctttgagat attctttcag ccatattcgc tccataagct taattgtttg ttctgaacct
     2941 aacgtgaata agtatcgtca atcagtatcc tgcttatgat tgccgttaca gaccctgatt
     3001 actgtcatct ttgatacgcc agccaggcgg gctgtttcac gcagtgattt tccgtgggcc
     3061 agacgcagag tacgtatcag ttcatgtttc tgtgcatcgg ccaccctgcc ccggtatttt
     3121 ccttcagttt tcgctttaca gattccctct gcctggcgac gtcgacggtc ttcgtagtct
     3181 tttctggcaa tagcggcgag catatccagc atcatgccat ttacggcctt cagcatgctt
     3241 cgggtgaagt catcacttac tgtgctggat agcgccagat ggctggttgg taaatcaaga
     3301 ctgacgatgg ccagctgctt gtcggtgatt tgctttctga gtgtctccca gcctgcatca
     3361 tccagtcgag taagcctgtc gacctgttca atgagaatca catcaccagg ttccgcatca
     3421 ccaagaagcc tgatcagttc aggacggttc attgtcgtac cggagacatt gtcgatatac
     3481 cagccagcaa tacggtggcc attttggttt gcaaatgcct tcagtgcatt ttttgcccgg
     3541 ctggcattct gctctgaagt ggatgcccgg aggtaaccaa aaacgaacat attttgtcct
     3601 tctggtaact tttaagtggt cttgcttatg atggtatcac ttatgtggta acaataatat
     3661 tcgccggtat tttttcgggt ggtatatata gagcataccc caatgtgaca ggctgacgcg
     3721 cccacggagc ttgtccaacc cctcgcctgc tcagatgcaa gctctgaaca gactcacggt
     3781 tttttctttg agtacagcct cagttgtctt tttgagctac gtgataggta ggtattaacg
     3841 tacaataatt taatgtcgta tcattatatg ttgtacgtta ttatttattg tgtacaatgg
     3901 cgtgagcttc tacgccatca atgtgcaagg attccatctt taggggacaa aaatgaaacg
     3961 cgattacggt agtgtgggta ccatagctct tagagcaagc gctttacttc aggctatgag
     4021 tagggatatt gaggaacaaa ggaaagaatt taatctgaca gattatcatc agacatatac
     4081 tcgtaacgct gtcgcaaaac taccaaagct gagccggcgt atcgtagaac tggccatgaa
     4141 agaaatggaa gaagacggct atatatttaa taaaaagcag attggtaacg tcgaacaata
     4201 tgctctgacg ataaagaatg ttatcgatat ctacgcccac cgacagatac ctaaatatcg
     4261 cgatatccat aaagaaccct acgttatttt cgttgtaaac cttaaaggtg gtgtatcaaa
     4321 aacagtcagt acagttactc ttgcacatgc gttgcgtgta catcaggacc tgctgcggca
     4381 tgatcttcgc atccttgtaa tagacctcga cccacaggcc tccagtacta tgttcctgga
     4441 tcacacccat agcattggta cagtccttga aacagctgct caggcaatgc tgaatgatct
     4501 cgatgttgaa acattgcgag aagcagttat tcgccccacc attattcctg gtgttgatgt
     4561 catccctgct tctattgatg acggctttgt ggcgagccag tgggagtctt tagtagcaga
     4621 acatttacca ggacttaaac cttctgaagt cctcaggaaa acaattattg accgtatcgc
     4681 tggtgactat gattttgtct tcatagatac tggcccccat cttgatccgt tccttctgaa
     4741 tggtctggca gcgagtgatt tgcttctgac ccctactcca ccggcacagg ttgatttcca
     4801 ttcaaccctt aaatatctga cacgtttgcc tgaaatgctg gagcgtcttg aagacgaagg
     4861 tgtggaacca cgcctgagcg cgagtatcgg tttcatgtca aaaatgacta ataagcgtga
     4921 tcacgaaact tcgcatagcc tggcccgcga agtgtatgca agtaatattc tggactcgtc
     4981 actgccacgt ttggatggat tcgaacgatg tggtgaatct tttgacacta ttattagtgc
     5041 taaccctgtt tcataccctg gtagtgctga agcgttgaaa aaggcacgta ctgaagcaga
     5101 acggtttacg aaagcagttt ttgaccgcat tgaatacatc cggggagagt ctaaatgaaa
     5161 aaggttttag ctcgtgggcg agttctcggt aatagcaatt cagagtttgc ccgtatgctt
     5221 gaaggtgacg gagatgtcaa aacgtttact cttaaatccg gcaagcaggc caaatttgtc
     5281 aaaacggttg tattaagcgg agagattgaa tcaaagacgt tcgttgatgc atcggtcaat
     5341 ggacgagatc aggctatgct tacgcgtgag tccgttagtg atatttcccg gacgataaag
     5401 ctgcagcaat tcttcccggc tattggacgg gaggttaatg gacgaattga gatattagat
     5461 gggacccgac gccgtgccgc ctgcatattt aataacgtta aattcgaaat cctagtaact
     5521 aaagatgata tatcactcgc cgatgcacgg cagttagcgc aagatatcca gacagctaga
     5581 gaacatagcc ttcgagagtt ggggaaacga cttgaagtca cctatggaac tagcatgacg
     5641 aaagaagaga ttgcgttgaa agagaatctc tctccggcga aagtgactcg cgcgtttcag
     5701 gccgcagcag tgccagatga aatggttgca gtattcccgg tgataaatga tatttctttg
     5761 tcggattatc agtttttgct aaaactggct gaagaagcaa acaacaagca aacatccgta
     5821 acagaactga tggaaaaagt tcaacatcgg ttgaagacca tgccagatta tccggcaatt
     5881 gataaaagca aaattctggc ggctatcagg gttgagagca aatcgttgac aacccgccct
     5941 accagaacgg ttcaaactga gaagctgaga gaattttcag atcgcaatca gtttgccaga
     6001 aagaaaactg attcaaagaa gcgactggtt gtttatgagt tttcccgcat ttccgctgag
     6061 gcacagtcgg agattgatga tgcaataaaa cgtattctga aaagacttcc tgagtcaagt
     6121 gagtaaggga aaaggatgcc ggagagcatc ctttttttat gtttttcacc atgccgattt
     6181 caatgttatt attatgattg taaaggaaaa ttttaaattc tttcacactg aaatcaccaa
     6241 gtttttcaac cttttcgtga ctcataattt cgctactggc agttcagacc agcgagttgt
     6301 ataggccggt gaaagcatct cccgtttcat ttgccactca ggtgcgatac ctcgtcccgc
     6361 aaaccatact ttccccagac cggaatggtt aataccgtcc atgactttca tcagctgatt
     6421 actgttgctt cggggctgag tatcatcgaa caggttcaac tgcgaaaccc cggtaggagt
     6481 gaaatcattg agcatgcaac ccgcttttgc atatcgatgg ccattcaccc agatccgatc
     6541 cagtgctttc accgctgcgg ctatgatgtc cctggtatcc tgcgtgggag tcatcaactt
     6601 ctcactggcc acattcccgt aatacggttc gttcaccgcg aacggtgacg tcttcacaaa
     6661 tacagcaatg tgcctgcaga actgtcgctc gccgcgcagt ttttcagctg cacgctcagc
     6721 atactgacaa actgcctggc gcatggcttc atacgtcgtg acacgctccc caaaggaccg
     6781 gctgcagaca atctgctgtt ttggcggcgg cgcttcttca agcgaaatac agctttctcc
     6841 gttgagttca cggacggttc tctccagcac aacgctgaaa ttcttcctga taaatacagg
     6901 gttcgcgcgc gccagctgca gtgccgtagt gatgcccatc gtattaagcg tttttgagat
     6961 cctgcgacct actccccaga tttcttccac cggctgtcgt gagagcagtt tatccgttcg
     7021 ctttggattg cctgaagtca gagcaagcac gcccccaaac tgccgccatt ccttagacgc
     7081 ccattgtgcg cttttagcca gcgttttggt cggccccatc ccgacgccga ttgtcagccc
     7141 cgtaccggag cgcacgtgct cgcgcagctg ccggccgaag tcctcataat tgatgcagct
     7201 gtctataccg cggatatcca gaaacatctc gtcgatgctg tactgctcca cgcgcggcgc
     7261 cagttcttcg agatgagaca taactctgtt cgacatagac gcatagagct cgtaattgca
     7321 cgagaacgtg atgaccggct ccgggaattg tgccgtcttc agctggaacc aggggacgcc
     7381 cattttaata cccagccgtt tcgcttcggc actgcgcgcg atgacgcagc catcattgtt
     7441 actgagcacc acaacgggcc tgttacgcag gtccggacga aatactttct cacagctggc
     7501 gtagaagctg ttgacgtcag ccaaagcgaa catcaattcg taccccgcgt cttatgtatg
     7561 aatgccatca ccacgccgaa tatctgcagt tcttccggat aaagagtggg gtaggcggga
     7621 ttcatcggca gcagggcgat acggggttta agctgcaggc gcttaacggt gaattcgcca
     7681 tctgtttccg cgatgacaat atccccctgc atgggttttt cagctttgtc gacaaccatg
     7741 agatcgccgg agtgcaggcc catatctttc atcgaatcgc caatggcccg gacaaagtag
     7801 gtcgcgctgg ggcgacggat acagtaggcg ttcagatcca gttcgtcttc ggtatagtcc
     7861 gcggccggtg acggaaaacc agccggacag cgctctgtga aaagtggtgc ggtggacagt
     7921 gctggctctg atgcgggagt gacaaaaagg atcatggcat acctcttaaa atacctgttt
     7981 ttacatacag tgttatttaa gatatttccc gtcctgcaag gagaattttt cctgttcctg
     8041 ccaggttatt gttctggcag ataaaacagg cagtgggtta tccacagaaa cggtgcataa
     8101 ccctgtgagt cgatgccggg tatctcaccg tctgctaagt cagtggctct ataagttccc
     8161 ggccctggtt cccgggattg ccaacagccc ggctgacggg gtgccatgtg aaatcttcgg
     8221 cgggaagact gccctcacgg gcaatgtctt ccgcctcttt accggttgtg ttttcgctga
     8281 gccatttacg tgcggcatcc ggtgagtaca cgagtggtcg ccggtcgtga atgtccagca
     8341 gttcctcatc cgcagcggcc gtgacgatca ggaatccttc ctggtcattg ccgttctcaa
     8401 aaggtggttt cccgatggca gcgagcagga gcggatttcc gtccttacgg taaatgaaaa
     8461 acggctgttt cacgccgttt tgctgtttcc actcaaacca gccgtcagca aaaacaatgg
     8521 cccgtccgtg ctcccagagt ggcctgaaca tccggctgcc ggccgcagtt tcacttctgg
     8581 cgttgatgag agcgggcttg ttccaccact ccggcgcata accccagtga acaggatcaa
     8641 gatgcagctg cgagcggcgt tcactcagca gtaatacacg ggttccgggc gcaacgttgt
     8701 agcggccgac gggctccgga tcatacggga tgtcccgttc tgcttcttct ccgagaaagg
     8761 ccaggtactc ctcgcgggag ttgtactgcg taaaccgacc gcacattatc gtttctccgg
     8821 tgtagatttc gcatgcgaaa tctgtgtttt catcagttca gaaaataggc cgtttttcag
     8881 gttcctgcca tcgtgtggcc gccgtgccgc tactgtagcc gggccgcttc gaatgcaagg
     8941 gttcgctctg ccggtgtcct cacccggtcg cagtttattt caaaggcttt acccgctgtc
     9001 ttcccgtcgt tttccgtctc aattttcgcc gtcaaaccgt ccagccagcg tgggcttttt
     9061 cactgcgctg cggcttgcgg caccgcccct gcattctcca cttttcgccc ggctgtcgta
     9121 tcggcacggc gtaactggcg ccgctttgag aaaggagaac tactatgtcc cgatttatcc
     9181 agggtaattg cgtccatatc atgtccggct ttccggataa tgctgtcgat ttcattctca
     9241 ccgacccgcc atatctcgtc ggttttcgtg accgtcaggg gcgtaccatc gccggcgata
     9301 aaactgatga atggctgcaa cccgcctgcc atgagatgta tcgcgtgctg aaaaaagacg
     9361 cgctgatggt gagcttctac ggctggaacc gcgtcgatcg ctttatggcc gcctggaaaa
     9421 atgcgggatt cagcgttgtc ggccacctgg tgtttaccaa aacctacaca tcgaaggccg
     9481 catatgtggg ctatcgccac gaatgcgcct acatccttgc aaaaggccgt ccgcccctgc
     9541 cgcaaaaccc gttgaatgac gtaattgcgt ggaaatattc aggcaaccgg caccacccga
     9601 ccgaaaaacc cgtaaccagc ctgcaaccgt tgattgagtc cttcacacat cccggcgcga
     9661 ttgtgctcga cccctttgcc ggcagtggct ccacctgcgt ggccgccctc caggctggcc
     9721 gtcgttacat cggtattgaa ttgcttgagc agtatcaccg ggccggacag cagcgtctgg
     9781 ccgccgtccg gcgcgccatg cagtacccgg ccgctaacga cgagttcccg gaggccgcgt
     9841 aatgaactat gcaggacacg aaaaactccg cgcagaagtg gcggaagtcg ccaacagcat
     9901 gtgtgacctg cgggcgacgc tgaacgggat ggagcaccgc tatcgctttg actctgatgt
     9961 gctggccgaa cgcctgaccc gtcagaccct ttttcgcatc aatgccctgt ttatggcggc
    10021 atacaacgaa atgcttgagc tggatgcctg ctttaaggac taaggagaaa aacatgtacg
    10081 gaacctgtga aaccctctgc cgcttgttgc gtgagcagta tcccgcagaa acccctttga
    10141 acctcattgt ctggtccccg gcggatatcg aagcactggc cgacggaatg gaatatgccg
    10201 tttcggagca ggacacaagg gcggtactgg cgcgtatgga cgccattccg gaggaacaac
    10261 ggcttgagtc gggcgtgtct gccggtgcgg tgatggatct gattgaacag gtgaaagagg
    10321 ccgttccggc ggtgatggtg ccggcggacc tgcttgaaac cctgctgacc actgccgaac
    10381 aggcgttatg gcacagagaa tggaccgccc gtgactgcaa tcatccggtc ccggaaagcg
    10441 ttacccgtcg cctggctgat gcggcgaaag ttcgcgcctt actgaaaaaa tgaaatcaac
    10501 acgccgcccg ggcggcgtgt actgaccccg ttcagaggaa cccattatgc aggaaaccac
    10561 cacgttaaac gcccttgtta tgcgccgtgc gcgcgatttg attgctgatt atggatggcc
    10621 tgaccatacc gatgttgatc agcgtgatcc ggtcaataaa ccgggctgga taagcattta
    10681 tgtccgtctg gatgcggcaa atattgttca tctgctgcct ttgctttgta ccggtgacgt
    10741 acccgcagat ctgcaaagtg ccatgacaaa aatatccggg acgccagcgc agattatttt
    10801 atccggcagc cgctatgccg acgcgccgca gcttccggag gacggaacac agatagcttt
    10861 cccctgggcc ggggaatggc tgacggagcc ggagattcag gctgtaacgg attgcctgtc
    10921 ccgcgccgtg cgggatgtat cccggcaggt atgggaggat gcgcgccgga taaaggcggc
    10981 actgaacacg cgcggggaaa cgctgtttta tcgccagacc cgaaacttcc gtctggttgt
    11041 gaaggaaaat gatatgccct gctggctgga cgatgatgac aatttgccgg tggtgctcga
    11101 tgccatcctg aacaaagggg cacgctacag tagcgtcgag ttctttgtta tcagcgacaa
    11161 agtggatcaa atcctggcat gtggccagat gtgcgatgtg ctgcgtattc cgggcgagcc
    11221 tccgcgccgg tggatggatt taaccctgtt acatgaggtc atggcagaag cgcgtgcaga
    11281 aatcagtctt gtccgcaatg ccctgtcagc aatccggcca gtttagcgaa cagcaggggg
    11341 cggcggcccc ctgaatctgg cacgaaggat aaggatgtcg ccggctccgc ttttacggct
    11401 cttcctccac gttgttccgg cttcgcgttg agtaacgtcc cgtgcggcct gcggcaaacg
    11461 ccgtgcctct actgtagccg ggccgcggct gatgcaaggg aagcgtaaac gccgtcacct
    11521 tcacccggtc gcagtttatt tcatacgtta tccgccgtct tcccgtcgtt ttccggctca
    11581 gttttcgccg tcaaaccgtc cagcctgctc ctgctttttt actgcgctgc ggcttcggtg
    11641 acgcccttgc atcctccgct tttcgcccgg ctgtcgtgtc ggcacggcgt aagccggaac
    11701 tcagacgaca tcgactaggg aggatttttt atgcgcacta caaccaccac acccgctgtt
    11761 tacgtgggca cgtatcacaa atacaactgc ggcagcattt tcggcaaatg gtttgatctg
    11821 acggaatttg acggcaggga ggatttttac gaggcctgtc aggcgctgca tgccgatgaa
    11881 tgggatgccg agtttatgtt tcaggactgg gaaggtatcc cgtcgcagtt cgtctctgaa
    11941 tgctccattg actgggattt tatcgccgct tacaaacgcg ccgaagaaga gggcagggaa
    12001 gccgctttta tcgcctgggc ggagtatacc ggcgaatgtg actatgacgc gtttgatgat
    12061 gcgtaccgcg gcgaggcgga aagcgaggaa gactatgcgc gggagatggt ggaggacaac
    12121 ggcctgttaa atgaggtgcc ggagccgttg cgcagctatt tcgattttga agcctgggcg
    12181 cgtgacctgt tcagcagcgg ttatgtgttc catgacggct atgtcttcag caactgatta
    12241 atgtccggcg ggcaaacccg ccggttaagg agaaaacgta tgaaacttgc accggaaacc
    12301 cgcgagatcc tgcgacagta caaagccctg attaatgccc gccgacgtga tgccggtcag
    12361 cgggaactga ccaccgcgca ggtgatggat gaaatctgcg aatacatgac ctgtcagtgt
    12421 gccgtgtaca tcggcggtca tttcatctta cagggtggaa aaggccagta agtatgatgg
    12481 caggcgtccg ggccggaggg cgcctgtcag cccgcgcgaa aaaccgggca ggctgcgcct
    12541 gccggagaga aacttcgccg cgcctgcggc gccgcgcact ctcctctcca ttgtcagtcg
    12601 ctgttgcccg ggaatgtgcg tattatcagg gatatcttcc tgtaaagcag accggtgatg
    12661 catatgtcga agtggaatat cgcctctttc tcaaaagaag atcaggataa ggtctcggtc
    12721 gataaagtcg ccgctgccgt tgcctggcag gaaaggatga acaggcccgt ggtgccggag
    12781 ctggctgaac gggaacagcc tgaacatctt cgccagtatt tccgtgaacg gcttgacgtt
    12841 catcggcaga acagtcagca gctgccgcgc gcaaatgcgc ccgaatatca taaacccgga
    12901 gatgagcagc agaagtgatt cttatagtca gtgtgtccag ggaggaaatt ttatgcgtat
    12961 gtctctgatg tttgcggccg tgactctttt aaccggtatt aacctgttgt ttattccgtt
    13021 tcagcctgta ccggctctat gggggggaat cttggccgtt gcttttatcc tgattaatgt
    13081 cgcggcggat aaactgcgtc tctgaggtgg cgttgcatgg attatcgttg ccggcatcat
    13141 cataatgttg atgctgaagg ggaaggaacc gggggacgaa ttaagagtgc tgatcaccgt
    13201 ttttgctttc ctggcttcgg cctggtatag cgttgtgcag tacagaaaag aaaagcattt
    13261 tcgctcctga tgttcccggc tggcatcaag ctggatgatc gtcagccatc attttagccg
    13321 catgtaatcc attcttatcc tcccgttttc tgttttctgc ccgttcccgt tttcattgcg
    13381 ggcgtccggg cgggctgtcg tgaacggagc cggtaaaccg tctccgccct tcgggcttcc
    13441 atcccatgac gcaacaccag ggcttgcagc atccttatca ggatgcgccg cctctgtcgc
    13501 gccgtggcct gcgctccctg caccccggcg aaaatcaacg gccgccgggc tgtaaggggc
    13561 aacgccgttt cgcgcccgcg ctttccgtcg ttgcggcggg ttcctgctca ttccttgcct
    13621 ggttttttca cccttttgag tcctttcgcc ggggctgaga caagcacagc cgctgtcagc
    13681 tgtccagggt aaatggcacg cggtaaaccg cgccccggac atccgccatc gcccgcgctg
    13741 gtttacgccc ctgcggcgaa cggctctcaa acgggtgaag gtaacaacca gcgtgggcgt
    13801 tcagcaggtc cccgcaaaac gtcaaaaccg cggccccggc aactcaacgg gtgatggttt
    13861 tttaaaagca aaacaggttc agaaggaggg gcaacatgag cgaggcgctg gcggttttac
    13921 ccgacgacac ctttacccgc gaacaggctg aggtcgttgc ggcgcagtac accaacgtgg
    13981 caattgagga cgatcagggt acacattttc gtctggtcgt tcgtcagaac ggcgtaatgg
    14041 tctggcgcgc gtggaatttt gaagcggata ccgggaaatg gctcaaccgt tacatcgaac
    14101 gttacggtat tcgtaaacag cagtaaataa gaaggtgccc tgccggagcg gtaactccgc
    14161 agggccggac aatcatcaat cagtgaggta tcaactatgt cagtaaccga tgttaaagca
    14221 aaagcaccca aaaaagcgag cagcaaaaaa attacgaagg cacaggaggc tgccctgaag
    14281 atcgcccttg aggccgctgc ggtcgagtac gttccgctgt ccgatctcgt taaatccccg
    14341 ctgaacgtgc gctccatccc ctattccgtg gacagcgttc gcggtctggc cgactccatc
    14401 gagacgctcg gactgctgca gaaccttatt gttcacaccc tcgcggacgg acaatccggc
    14461 gtggccgcag gtggccgccg attaactgcc ctgcaactgc tggcgcagga agggcgtctc
    14521 gctgctgacc ataccgttat ggtgaagcgt gtttccgacg acattgccgc ccttgcctcc
    14581 gtcgccgaga acgagcagcg cgccgccatg catcccgccg agcagattgc aggtttccgt
    14641 acgctggcag ggcagggcaa aacccccgcg cagactggcg acgcgctcgg cttcggctcc
    14701 cgtcacgtgc agcgcatgct gaagctggca aacctcgccc cctccctgat ggagaagctt
    14761 gcacaggacg aactgaccgt tgagcagtgt caggcgctct gtctcgagga cgatcctgcc
    14821 cgtcaggtcg aggtattcga aaacgtgaag gccagctagt caaacgcgcc cgcacacctg
    14881 attaaacgcg ctattaccga aaccgagatg cgcaccgaca acgccaaatt tcgttttatc
    14941 gggcgcgatg cctacgaggc ggcagggggt tacgtccgtg aagatctgtt cagccaggac
    15001 gagggcgacg gtacggctga cagcgtgctg gttgagcgtc tggtgcagga aaagctggag
    15061 cgtatcgcgc aggacattca gcagcgggag ggctgggcat ggagtcgcgg acgcgcagcc
    15121 cgcatctggt accacggcga agacggtaag gagttcgtgc agcctgctga acccgatccg
    15181 gtgtacaccc ctgagcagca gcagcgtctt ggtgcgctgc gggagcagta cgatacatat
    15241 gacagcgttt gcgacgagtc agatgcgatc gaagcggaca tcctcgccat tcaggcggcg
    15301 gcagaagcca gcgcgtggac tgacgacatg aagtcaggcg cgggagtgat ggtcagcctg
    15361 tacgaagggc aggtgtacgt gcagcgcggt gtgcgcctga aagcggatat gcctgaagaa
    15421 accgtaacca gcagcgtaac ggtgccattc acctcacacc agcccgacgc cgcagagggg
    15481 atcagtgttc cgctgctcac gaaaatgacc tccgaacgta cgctggcagt ccaggcggcg
    15541 ctgatgcagc agcccgaaaa ggcggtggcg ctgatggtct ggcgcatgtg tacctgcgtc
    15601 ttctcgggct gcctgaccac gacgcatccg ttccgtatca gcctcactgt atctcacggc
    15661 agcctgacgg agaacgcccc gtccggtaag gatggcgcag cgtttaacat cctgatgacc
    15721 gaacgggcaa ggctgaaagc cctgctgccg gaagggtggg agaaggactt caccaccttc
    15781 tttgcccttg acggcggggt gttgatgtcg ctgatggcct tctgcacggc ctgttccgtt
    15841 gacggggtgc agacccgcga tatggggcac acctcgcgca gtaccctcga tgcggtcgag
    15901 gcggcaatcg gcttccacct gcgcgactgg tggcagccga cgaaggacaa ctacttcggc
    15961 agcctgaaac atccgcagat tgtcgcctcc ctgaaagagg cggggctgac gggcgcggcg
    16021 ggtgacgcgg agaagatgaa gaagggcgat gctgcagcgc atgcggagca ctttatgcag
    16081 cacacccgct gggttccggc atggctcaaa ggaccggagt cagcgggtga atccggtgca
    16141 gacgacgcgg cttcagatac cgacagccct gacaacgaca ccaccaacac ggcacacgcc
    16201 gcctgataac ggagcgccgc cctgcggggc ggcgataagg agatacccat gcgcactatt
    16261 ctgacccccg atgtactgaa aacgatgtcc tgtgatgagt ttgaggactg gcgggacagc
    16321 ggcgaagact tccgccgcga actgacccac gccgtcatgc gtgacctgtc ctgccctgaa
    16381 aactgggatg tgaacggcga gtaccgcagc gagttcggcg gcttcttccc cgtacagata
    16441 cgctttaccc cctcgcacgg caattaccat attgcggtgt gcagtcctgg tgccatcagt
    16501 ccggcgtgga tggtggtgtt cgtgcccgcc agcggtcgcc cgttctccgt cattcgtacc
    16561 ctgagcggct accagccgga gcttgtcagc cacaccgtca gcctgacggc gcgtctcgat
    16621 gcggacggat acagtcaggc cagcatcatc agtattctgg cagcggaggg cgcggcatga
    16681 tacctgcaaa cctgtcactg gttcccttca gccctgagcg acgcgccgcg atggaggcca
    16741 tcgcggaggt tgaacgccgg cggtcgcgcg gcgcggtgtc cacgatgtat ccgtatgcca
    16801 gcgccttttt ccggcggctc acgggcagca cgcgcatcac tatcagggaa atccatcatt
    16861 ttgcgccggt gctgacggcg cgggagctgc ggggcagcag ggacagctgg ctcagtgcca
    16921 tcgatgcgct gattgaaagt cgcgggacgc gttgctggct gccgcttccc gttggtgcgg
    16981 gtcagcgcct ctttccggag gtggcctttc agcagaccga gcgggttcgc tgtcaggacg
    17041 ccctgcggga tgaaaaatac acccgacagc gtcacaaaga acggtgccag cgtgagcagg
    17101 cgtaccaggc gctggcgggg caggcggaga ttgagctggc attccatacg cctgaaacgg
    17161 tcagcagctg gagcgcacgc tggtcgggga ctgaactgcg ccagtacgac ctggaagaga
    17221 tgttctggcg ctggagcgag cgtttcccgt cactggagcc aatggagcgc tggacgatgg
    17281 agagtcagcc tttctggacg gtgatggctg aaacgaatgc gctggcaagg gaaagtccgg
    17341 gatctgtccg gcagctggag cgctggatgg tacccaacaa gctgacagcg cggagtcagg
    17401 tatgaagtgg cgttactcgc tgcgctggaa gcgtcccggt ccctgtcccg gtgaacctga
    17461 actggcgtca gaagtggtgg aggccgggaa gccagcccct gagtccgtga tgtcactgtg
    17521 ggtggcagga gccggatatg ctgtctgcgt tgattttctg tatgaacggc cgatcaggcg
    17581 ctggagcgat gagcgaaagg cggccacgcg gcgacggaat ctggcgcgtc gtgttaacag
    17641 gatcgcgccc ctgtttgcag atgaactcat tgagcgggaa ctgacggtgc gaccggatta
    17701 ttttcgggga aagtcgcctc actgagtctg atatcatatc ctgccagaat acggtcggca
    17761 gggtgcttaa aagggataat ttagagatga gatactggat actggtcgcg ctgttaaatg
    17821 gcctgtttac gctggtcata cattacgcgg cgccggagat gttgggaggc acggcgtact
    17881 atgccggggt ggcaatctgt gccctgattg cgctggttat cacctggcgc gatccgccag
    17941 gtatgctgga caacgtgctc ttgtttctcc ttaagtgggg aattgtggct tcaggtctgg
    18001 cgttttttat ctggggtatc ggatacaagc tggaattctg gggagatcat aaccccgatg
    18061 aaattggcgg tgctgagcgg tgttctgctt ctggtcagtg gctgggtgta ctggaggcga
    18121 aggtaacaaa ctatacgcca gcgtgaggca ggaaagtaaa aagagcggtt atgcctgcgg
    18181 tataactgct cttttatatg gtcctcacgc tttgtaattt cctgctgtga atcacctctg
    18241 aagaacgaac gctgcgaatt atggtggcag ttgtctgtat catcttttca aatttgcctg
    18301 ctgtctgacg gggtcactgg ccatgactgc gggcgacctc cctgacggga accggggctg
    18361 tcgtaaacgg agccggtaaa ccgtctccgc cctgacgggc ttccatccca ctgtcgcaac
    18421 gtcaacaccg gctcgcagtg accgttccgg tcacgccgct ttcaccatgc cggcgataaa
    18481 gccgccggca ctcaggtgac gccacgtcgt tccgccgtgt cgggtgtgtt catcctctcc
    18541 ccgtacctct ggcgagccct ctcccgttaa gtccccgggc ggagggcttg tcgtagcacc
    18601 gtcgtgtcgt catccgaagg gtgaaatgca ctccgccaaa actttttgcg agggaccgca
    18661 aaaactttag tctccgccct tctcctgccg ccccgccgtt gctcctttgc cctttgccgc
    18721 cagggaactc accgagagat ggataaacca ccagacagga ggaaaagcag gatgaacaca
    18781 cagaacgtca acgtcaaaac cgccaccaaa gaatcttccg aaagatgggt gaaaacctcg
    18841 tccctgcgta ctgatggctt tgtgcgcaat atccattccc gtaacccgtt cgatgttatt
    18901 cgcgcggacg tggtgctggc ccgtatggaa atacaggcta accggggatg tggcctgcac
    18961 tacgagattt atgagtcccg tctgctgggt atggctatgc attacctggc agaactgcca
    19021 ctgaaagacc gtccggcgtt tatgggggcg gcagcgaaac ggggttacat gctgacgact
    19081 gcggaagaag aacgtgccga tggtgaatgt gctgacctga tgatcgagct ggcggcggat
    19141 tactgattac ttaaccggct ggcggcaggg gatgccgcca gtcacaccgg agaaacatta
    19201 tgagc
//
LOCUS       DACADA010000078        19084 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.079, whole genome shotgun sequence.
ACCESSION   DACADA010000078 DACADA010000000
VERSION     DACADA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.079"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            211..981
                     /locus_tag="ISB17_002770"
     CDS             211..981
                     /locus_tag="ISB17_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAP1980679.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            972..1445
                     /locus_tag="ISB17_002771"
     CDS             972..1445
                     /locus_tag="ISB17_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP1980680.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1552..2091
                     /gene="dnaT"
                     /locus_tag="ISB17_002772"
     CDS             1552..2091
                     /gene="dnaT"
                     /locus_tag="ISB17_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAP1980681.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2094..2831
                     /gene="dnaC"
                     /locus_tag="ISB17_002773"
     CDS             2094..2831
                     /gene="dnaC"
                     /locus_tag="ISB17_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAP1980682.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2880..3374
                     /locus_tag="ISB17_002774"
     CDS             2880..3374
                     /locus_tag="ISB17_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAP1980683.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3628..5919
                     /gene="opgB"
                     /locus_tag="ISB17_002775"
     CDS             3628..5919
                     /gene="opgB"
                     /locus_tag="ISB17_002775"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAP1980684.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLTWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6059..7081)
                     /locus_tag="ISB17_002776"
     CDS             complement(6059..7081)
                     /locus_tag="ISB17_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1980685.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEVVGLGKNVADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7220..8134
                     /locus_tag="ISB17_002777"
     CDS             7220..8134
                     /locus_tag="ISB17_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1980686.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFEQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENMRYAH"
     gene            8351..9712
                     /locus_tag="ISB17_002778"
     CDS             8351..9712
                     /locus_tag="ISB17_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980687.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9761..11425)
                     /gene="tsr"
                     /locus_tag="ISB17_002779"
     CDS             complement(9761..11425)
                     /gene="tsr"
                     /locus_tag="ISB17_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP1980688.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLHDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVMPAAPRKMAVADSGENWETF"
     gene            11802..13952
                     /gene="btsT"
                     /locus_tag="ISB17_002780"
     CDS             11802..13952
                     /gene="btsT"
                     /locus_tag="ISB17_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAP1980689.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14002..14205
                     /locus_tag="ISB17_002781"
     CDS             14002..14205
                     /locus_tag="ISB17_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP1980690.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14216..15172
                     /gene="yjiA"
                     /locus_tag="ISB17_002782"
     CDS             14216..15172
                     /gene="yjiA"
                     /locus_tag="ISB17_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAP1980691.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVISTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15206..15508)
                     /locus_tag="ISB17_002783"
     CDS             complement(15206..15508)
                     /locus_tag="ISB17_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HAP1980692.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(15495..15782)
                     /locus_tag="ISB17_002784"
     CDS             complement(15495..15782)
                     /locus_tag="ISB17_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HAP1980693.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIISARKADRKERSRYEHG"
     gene            15979..17145
                     /locus_tag="ISB17_002785"
     CDS             15979..17145
                     /locus_tag="ISB17_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP1980694.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIRDQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRGMVTREGMSYCITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            17209..18828
                     /locus_tag="ISB17_002786"
     CDS             17209..18828
                     /locus_tag="ISB17_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004720069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP1980695.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTEDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            18818..>19084
                     /locus_tag="ISB17_002787"
     CDS             18818..>19084
                     /locus_tag="ISB17_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP1980696.1"
                     /translation="MGSKWCIYRLGDIAEFSYGKMPKKEFVGTGDYVIFSGYKYTESY
                     PEKNTSAGELIVVARGVGGTGDVKITTRDCYLTNLSIKINLDETK"
BASE COUNT         4657 a         4992 c         4765 g         4670 t
ORIGIN      
        1 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
       61 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      121 taacctacct caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      181 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaacg
      241 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      301 gatgagcttt cctcacgact gggtcgggcg ttgggaatgg acagcgtcga aagttctatc
      361 tcttcgaacg ccatagtgct gacaactatt aaagatgggc aatgcctgac atcgacacga
      421 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      481 cttgcggagc atcatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
      541 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      601 tgtaaactca ataacggtgg ctgggatggt gccgtcatca ccttctttgc cagtacgacc
      661 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccacagat caacttttgc
      721 cttaccgctt tcgccgccac caccatttcc ggattgcttt tgcaactccc cactttcagc
      781 gatacaccca ccattgcgat ggccgccagc gttctgctgc tggtgccggg ctttccgttg
      841 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      901 atcgccagtc tgctgacgct ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
      961 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1021 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct
     1081 ggtgtgcgct gcttggcgcg attggccatg gttcacgaat gatcttaatg accagcgggc
     1141 tgaatattga gtggtcaact tttatggctt ctatgctggt cggcaccatt ggtattcaat
     1201 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1261 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1321 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1381 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1441 tataaaattg cctcgcttaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1501 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1561 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1621 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1681 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     1741 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     1801 gtccccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ctttatccgc
     1861 ctggcggcgc tatggggcgt ggcgctaaga gagccggtga ccaccgaaga actggcctca
     1921 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     1981 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2041 aatacggtca gcgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2101 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2161 tcaaaacggg tgaagagcta ctggcctggc agaaagaaca aggggcgatc cgctccgccg
     2221 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2281 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2341 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca
     2401 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2461 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2521 aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg
     2581 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2641 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2701 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2761 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgt gtaacaggta
     2821 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     2881 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     2941 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3001 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3061 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3121 actgcgcgaa gcaaaagctg gcttcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3181 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3241 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3301 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3361 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3421 ctcctgccct tttccctgct cctggacggt tttacccctg ttacacggga agcgatcgcg
     3481 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     3541 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     3601 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     3661 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     3721 ttaacagtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     3781 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     3841 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     3901 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     3961 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4021 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4081 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta tggcgaaagt
     4141 ctcgagcgaa cctattttga taacgaggct ttcccggatc tcacgcctga actgggcgcg
     4201 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4261 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4321 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4381 aactcgggtt atcagaacta tttcgtgcag ggcgcaaatc tgcgttttgc cggtaaagat
     4441 gtgttcctga aatcgcacgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4501 gtagccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     4561 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     4621 acagtcgata cccatcaccc ggatggtttt atttctcgta cctgtaaccg caaaaaatat
     4681 gattttgacg gtaaaccgaa tcagtcattc agcgcggtaa gttgcagcca ggagaatatc
     4741 gcgacgttta tcaacaaaat caaagcgtca ccgtggttta aagatactgt catcgtcgtc
     4801 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     4861 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     4921 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     4981 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5041 gagaaaacat tgacgtggaa gccagatatc atccgcctgt ggaaattccc taaagagatg
     5101 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5161 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5221 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5281 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactttc caccgactgg
     5341 tgtgtctcgc aggggcagct tggcggtcag caaattgtcc agcatgttga caaaacaaca
     5401 tggaagagca agacggcatt taaagatacg gtcatcgaca tggcgcgtta caaaggcaat
     5461 gtcgatacgc tgaagattgt tgataacgat attcgctaca aagccgacag tttcatcttt
     5521 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg ggatttcccg tccggagtcg
     5581 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     5641 ctgccgaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     5701 cctattccgg tacgcgtagg caatgaagaa caaactcttg tgctgggcaa tgaagtgacc
     5761 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     5821 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     5881 ggcatggtgg aaattaaagt tgtggaacgt gaagggtaat tcctgactga tgtgtaggtc
     5941 gcataaggtg cgacctacac accttctccg gcctacaaaa catcattgag aaacagaaaa
     6001 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6061 agaaagtaat tacgccttta attaactcac gattgttaat cacatcgcgc tcgtaggttt
     6121 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg attttccctt
     6181 ccgccataag tcgaccgact ttagcgaaat cttccggcgt ggcgttgcgg ctgcccatca
     6241 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6301 cgacaaatac caccgtgcca ccgtgacgaa tcagattcac ggtgttattc atcgcatgtt
     6361 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgcagct
     6421 gcgcgtcaaa atcttcggct gacgggtcca gcacgggcaa ctccagacgc gttgccacat
     6481 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     6541 ttgccgccgc gcccagaccg attggccccg ctccgaccac cagcacctgc tcgccgggag
     6601 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg
     6661 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     6721 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     6781 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     6841 catttttaag atcagcaaca tttttaccca gcccaacaac ctccccacat atttcatggc
     6901 ctaaaacccg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     6961 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggaatct
     7021 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7081 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7141 aacggaaaat ctgttttgtg aaaacacgcg cataaaaatg ttttaaatcg ggttttaatg
     7201 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7261 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7321 ggcggaaatg tacaacatca gccgcactac agtgcgtcat attctcagcc acttacgcga
     7381 atgcggcgtc ttaacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7441 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7501 ttttttcacg atgataaacc agcgccagtt acgcccaggg gaaacttttt ctgaactgca
     7561 actggctcgg gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg
     7621 ccgttataat cttattcaaa gcgaaaagcg cggccagtgg agcatgaagc aattcgatca
     7681 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     7741 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     7801 tcacaggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     7861 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgaac aatcgcttga
     7921 gatcatatca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     7981 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8041 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8101 atccattaat gagaacatgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8161 agcgggtgca cggttttatc aaaagataaa taccggaaaa ttcccgctta cctatgctca
     8221 caatcagtcg actaaaaatg tttttttgtg agcggtagta aagtcctaaa actttaacct
     8281 gccgttaatg tcaaccttgc gtaatgcaca attacgtaaa caattaaaat tataaataca
     8341 ggagtccggc gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc
     8401 ttcgtcggca gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa
     8461 acgcattcaa accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga
     8521 ccgcagttcg ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac
     8581 cgaaatcggc gctttgctct ccgtgttttc actcgcttac gggattgcgc aacttccttg
     8641 cggcccacta ttggatcgta aaggcccgcg cctgatgttg ggactgggga tgttcttctg
     8701 gtcactgttc caggcaatgt ctggcatggt gcacagcttt acgcagttcg tgttggtgcg
     8761 tatcggtatg ggaattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga
     8821 ctggttcaac atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat
     8881 tggcgttgcc gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg
     8941 gatgtttatt accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta
     9001 tcgcaaccgc gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag
     9061 cgtcaatgcc cgccgagatc cgctcagttt tgccgaatgg cgcagcttgt tccgtaaccg
     9121 cacaatgtgg ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct
     9181 ggcctggctt cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt
     9241 gatggcggct atccctttcc tgtttggggc tgccgggatg ctggtcaacg gttacgttac
     9301 tgactggctg gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc
     9361 cgggatgttc tgttctgccg cctttacgct ggtcgtaccg caagcgacaa catccatgac
     9421 agcggttctg ctgattggta tggcactgtt ctgtattcac tttgccggaa catcctgctg
     9481 gggcttgatc cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa
     9541 ctttgccagc ttcatctgcg cctcttttgc gccgatcatt actggtttta ttgttgatac
     9601 cacccactca ttccgtctgg cactaatcat ctgcggttgc gtcaccgcag cgggggcact
     9661 ggcgtacatc ttcctggttc gtcagccgat caacgaccca cggaaagatt aatctcccta
     9721 tgcccgataa cattttgctt atcgggcatt tttatggcga ttaaaacgtt tcccagttct
     9781 caccgctatc tgccacggcc attttacgcg gcgcagctgg catcacggtt tttaccacag
     9841 ccgatgtttc acgctgctgt tgctgctgtt gctgaatccg gaacactgcc actgcttcgg
     9901 tcaggcgact ggcctgctct tccagcgcgg cggcggcggc ggcagattct tccaccagcg
     9961 cggcgttctg ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac
    10021 cacggctctg ctcatcagaa gcagaagcaa tttcgcccat aatgtccgtc acgcgggtca
    10081 cagcactgac aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat
    10141 ccactttccc caccgagtct tcaatcaggc ttttaatttc acgagccgcc tgggcgctac
    10201 gctgggccag attacgcact tctcccgcaa ccaccgcaaa accacgtcct tgctcacccg
    10261 cacgcgctgc ttcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt
    10321 caattacgct gataatatcg gcgattttct gcgaactggt ggagatatca cgcatagtct
    10381 gcaccacgtt atctaccact ttgccgccgc gttgcgccgt ttcagaagca cttaacgcca
    10441 ggtggctggc ctggcgcgca ttctcggcgt tctgtttcac cgttgcggtc agttgctcca
    10501 tgctggctgc cgtctcttcc agcgaagcag cctgttgctc ggtgcgcgaa gagagatcgt
    10561 tattgccggt agcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat
    10621 caccgacggt acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca
    10681 tctcattaga gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgaa
    10741 tgctgtcaat caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg
    10801 cgaagatgac cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt
    10861 aggaggcatt gttatcgctg acggcgatat catggagccg atcgttttgt tccatgtaag
    10921 ccacatactg cttctcgaaa ccgtcctgat atccctgagt cggctgatca aagaactcgt
    10981 tgattttgcc tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt
    11041 aattacgttt gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt
    11101 cgtaatccgc ccagtttttt tccgcctgtt tcagcgaaat actggcactc tgcatcagct
    11161 cagcaacggt tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc
    11221 ggttgagggt gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtggattgct
    11281 gctggcgaat ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaga
    11341 acagaccgcc tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg
    11401 tcacaatttt gatacgtttt aacatggttt ctctttcctg tggaccgatg caagattttc
    11461 ggcctggaaa ggaaaaactt tatgaatatc ccgcggagat tacgccgagt aattttattc
    11521 acactctgaa tttaaaaagc cattatttac ataaattatt cacaatataa atatgtgata
    11581 tgaatcacat atttatcgtc acttaaacga cgcctttgcc gctcaaccgc aaaactgacc
    11641 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg
    11701 gcaacatcac tacaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    11761 ggcttcggcc aactattaat caatacatgc caggttttac tatggatact aaaaagatat
    11821 tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc gcggtagttg
    11881 cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct gtatcggtgt
    11941 atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg aaactcgacc
    12001 ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt ccgaccaacc
    12061 gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg ctggtgggtc
    12121 cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg gcgggggtcg
    12181 tgctggccgg tgcggttcag gactttatgg tgctgtttat ctcctctcgc cgtaatggcg
    12241 catctcttgg tgagatgatc aaagaagaga tgggaccagt accggggact atcgcgctgt
    12301 ttggctgttt cttaatcatg atcatcatcc tcgccgtcct ggcgctgatt gtggttaaag
    12361 ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt
    12421 ttatgggtat ctacatgcgc tttattcgtc cggggcgtgt gggtgaagtc tctgtcattg
    12481 gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac gatccgtact
    12541 ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt ggctatgcgt
    12601 ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat ctggcaacct
    12661 tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg aacccggaac
    12721 tgaaaatgcc tgccatgacc cagtacattg acggtactgg cccgctgtgg aaaggcgctc
    12781 tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc cacgcgctga
    12841 tctcttccgg tacgacgcca aaactgctgg ctaatgaaac cgacgcgcgt ttcatcggct
    12901 acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgct gcgtccatca
    12961 tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc atcaccatgc
    13021 ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag ctgaaagacg
    13081 ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg ccagagcaga
    13141 tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc
    13201 cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg gctgacatgg
    13261 gcttctggta tcacttcggt attctgttcg aagcactgtt catcctgacc gcgctggatg
    13321 cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc atcccgttcc
    13381 tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt gtgggtctgt
    13441 ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag agcctgtggc
    13501 cgctgttcgg tatttccaac cagatgctgg cagccgtagc gctggtactg ggcaccgttg
    13561 tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg gctgtatggc
    13621 tgcttatctg caccacctgg gcgctgggcc tgaaactgtt cagcaccaac ccgcagatgg
    13681 aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt actgacctga
    13741 cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc aacgcgggcc
    13801 tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc aaaacgtggc
    13861 ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt ccaatcccgg
    13921 aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt cggcggggct
    13981 ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa atatctcggc
    14041 caggcggcga agatgttgat tggcattcca gactacgaca actacgtcga gcatatgaag
    14101 accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga acgccagaat
    14161 gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag agagcatgaa
    14221 cccgattgca gttaccctac tcaccggttt tttaggcgca ggcaaaacca ccctgctgcg
    14281 ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg aattcggcga
    14341 agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa cgctgaccaa
    14401 cggctgcatc tgctgttcgc gctccaacga gctggaagac gcactactcg acctgctgga
    14461 taatctcgac aagggcaata ttcagttcga ccgtctggtc attgaatgca ccggcatggc
    14521 cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc agcgttacct
    14581 gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga tgaaccagtt
    14641 caccatcgcc cagtcacaag tgggctacgc cgaccgtatt ctgctgacta aaaccgacgt
    14701 cgcaggcgaa gcagaaaaac tgcgtgaacg cctggcgcgc atcaacgccc gtgcaccggt
    14761 ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca acggttttat
    14821 gctggaagag aacgtcatca gcaccaaacc gcgtttccac tttatcgcgg ataaacaaaa
    14881 cgatatttcg tcgattgtgg tggaactgga ttacccggta gatatcagcg aagtttcccg
    14941 ggtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca aagggatgct
    15001 gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc tctacagcgc
    15061 cgactgggac cgcccgtggg gcgatgaaaa accgcatagc acgatggtgt ttatcgggat
    15121 tcagttgccg gaggaagaga ttcgggcggc gtttgcgggg ttgaggaagt aattatggat
    15181 gatggccgcc atgtggcggc ctgaattatt ttttgttctg atcgcgcagc atcgcttcac
    15241 gtaaaattgc gttcaggcga gtttgatagc ctttaccagg tcgcttcagc cattccatca
    15301 catcagcatc aatacgcacc gaagcctggg ttttcaatgg ccgataaaac ttaccgcgtt
    15361 ctgcgttaga ccattgctca tcgctggaag gcgggatatc actgtaatca atatcttcgt
    15421 cgaatttatt cgccagtgcc ttcagttcgg cctcatgctg cacatcgagt gtagagggtg
    15481 tccctcgttt atgtttaacc atgctcatag cggctcctct ctttacgatc tgcttttcgt
    15541 gcactgatga ttcgaataat ttcatttccg ctttcgaaac ggatggtatg cgcaaccaga
    15601 ataaccacga taccgtgaac caggccgata gtctgccagc gatactcacc gttttctatg
    15661 cgatcttgct gtgacaaatg ttgtggatca tcaaacacta acaccgcatc ttcaaagcgg
    15721 atgccatgtt tcacccgatt acttttggcc ttgtttgcat cccattcaaa ctccattggc
    15781 atagttgcca tccctaattg tatatacaaa aatgtatcta caattattct gcaattatag
    15841 cgttgatgtt ggaaatagca tcacgttttg gataatcaac catgaaattg acaatgagta
    15901 agttggactt gattcatcca atagaatttc tcgcctgaca tggcacacta aacacctcgc
    15961 tgtcgttaag ggattaccgt gaaaaactat aaagttatta ccccgctttt tcctacctat
    16021 gcacaggtta aagctatgat gaaagccgtc tcaggctatt ccctgaaagc ggtacgcaat
    16081 atgattaccg ccattcgcga tcaaaccggt acgccgcaaa aacccgttga ctggtcggaa
    16141 cctgacctgt ggatcagcga acgcttaacg ggcgaagatg ccgacatcgc ccgtcgtatt
    16201 tgggatacag ataatcacat attgaacccg cgccacagct acggctgcta cttattcctc
    16261 aactatcccc agtttgagct gatggaaagc acgccagatg atacctggca gcccacctcg
    16321 cgcgggcaaa aattcctgca agatgacgaa aaaacgctgc gttcactgga tgaccaggaa
    16381 ggcattctgc aactgcttga gctgctggct ggccgagaaa tgtcgcgccg cgccgattta
    16441 ttgccggaat ggcaggcctt tttgcatcag cattcgaaat tcgcgtctgc aagttcagtg
    16501 aaaagcacgc tctactcccg tctctataac cttattgacc gtggcatggt tactcgcgaa
    16561 ggtatgagct attgcatcac cgataccgga cgcgcctggc ttggaaaagc gttgccagca
    16621 cgaaaaagcg atccgcgtaa agagctgctg gaagcggtga aacgctacaa cgagcagcaa
    16681 aaagagctgc tgcgcgaaca aatcagcaca atgaacccgt acaaatttga acaactggtc
    16741 ggtcaattac tggaagcaat gggctacgag caggtcgaag tgaccaaagc ctcaggcgat
    16801 aaaggtgtgg acgtgattgg tcaggtgcag gtcggcatca ccactattac cgaagtggtg
    16861 caggtcaaaa gaatgcagaa caccatcact cgaccgtata tcgaccagtt gcgcggagcg
    16921 ttgccttacc acaaggccat tcgcggtacg ctcatcacca ccgggaaatt tgcagcgaaa
    16981 tgtgcagaag cggcgctctt tccgggggcc gcgcccatta cgcttatcga cggcgatcgc
    17041 ttactggaac tgttaatcga aaataacgtc ggcattcgcc gcagcaatgc agtggaattg
    17101 ctggatgtgg atttgcagtt gtttgatgag ctggagatgg attaaagggc gaaatccgcc
    17161 gcctcaacca ttacaatagc gacccaattt atctgaacag agttcatcat ggccaaagca
    17221 ccaactaaaa aagccaaagc aaaaaaaggc tttgaagaaa cattatggga taccgcaaac
    17281 caacttcgcg gtagcgttga gtcctccgaa tacaagcacg tggtgttaag cctcgtgttc
    17341 ctgaaattca tcagcgataa gtttgaagca cgccgcaaaa aaatgattgc cgatgggcaa
    17401 gctgacttcc ttgagatgga agtgttctac cagcaggaca acattttcta cctgccggaa
    17461 gaggcgcgct ggtcgtttat caaacaaaat gccaaacagg acgatattgc ggttcgtatt
    17521 gacaccgccc tctcgacaat tgagaaacgt aacccaaccc tgaaaggcgc gctgccagac
    17581 aactacttca gccgtcagaa tctggaaacc aaaaaactgg cgtcactgat tgataccatc
    17641 gacaacatcg aaacgctggc acacgaaact gacgttgaaa cgttgtcgaa agaagacctg
    17701 gtcggacgcg tttacgaata cttcctcggt aagtttgccg caaccgaagg caaaggcggt
    17761 ggagagttct acacgcccaa atgtgtggtc acgctgttaa ctgaaatgct cgaacccttc
    17821 cagggcaaaa tttatgaccc gtgctgcggc tcggcaggga tgttcgtgca gtcggtgaag
    17881 ttcgtcgaga gccatcaggg taaaagccgt gatatcgcgc tgtatggtca ggaactgaca
    17941 gccacgacgt ataaactggc aaaaatgaac ctcgccattc gcggtctttc agctaacctc
    18001 ggcgaacgcc cggcaaacac tttctttagc gaccagcacc cggacctgaa agccgactac
    18061 attctggcga acccgccgtt caacctgaaa gactggcgca atgaagccga attaaccgaa
    18121 gatccacgct ttgccggtta tcgtatgccg ccaaccggta acgccaacta cggctggatt
    18181 ttgcatatgc tctccaagct gtcggctaac ggcacagcag gttttgtgct ggcaaacggt
    18241 tcgatgagtt ctaacaccag cggtgaaggc gagatccgtg cacagatgat cgaaaatgat
    18301 ctgattgact gcatgatcgc cctacctgga cagttgttct acaccacgca gatcccggtg
    18361 tgtttatggt ttatgaccaa atcgaaggct gccgacccgg ccaaaggtta tcgtgatcgt
    18421 cagggcgaga cgctgtttat tgatgcgcgt aacctcggca ccatgattag ccgcacaact
    18481 aaagagttaa cggcggaaga tattgccaca atcgccgata cctaccatgc ctggcgcagc
    18541 acgccggaag aactggctgc acggattgcg cgtggtgaca gcaagctgga aaaatatgaa
    18601 gaccaggcag gcttctgcaa agttgcgacc ctgcaagata ttaaagataa cgactacgtt
    18661 ctgacaccgg gccgctatgt gggtgcagcc gagcaggaag aagacggcgt ggcatttgag
    18721 accaaaatgc gtgaattgtc gaagacgttg tttgagcaga tgaagcaggc ggaagaactg
    18781 gatcgtgcga ttcgccagaa tctggaggcg ctgggttatg gggagtaagt ggtgtatata
    18841 tcgcttaggt gatatagctg aattttcata cggaaaaatg cctaaaaagg agtttgttgg
    18901 tacaggtgac tatgtcattt tttcaggata taaatacaca gaaagctacc ctgaaaaaaa
    18961 tacttcggca ggtgagttaa ttgttgttgc ccgaggtgtt ggtggaactg gcgatgttaa
    19021 aataacgact agagattgtt atttaacaaa tttgtccatt aaaataaatt tggatgaaac
    19081 aaaa
//
LOCUS       DACADA010000079        18891 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.080, whole genome shotgun sequence.
ACCESSION   DACADA010000079 DACADA010000000
VERSION     DACADA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.080"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            7..729
                     /gene="sdiA"
                     /locus_tag="ISB17_002788"
     CDS             7..729
                     /gene="sdiA"
                     /locus_tag="ISB17_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAP1980697.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(797..1021)
                     /locus_tag="ISB17_002789"
     CDS             complement(797..1021)
                     /locus_tag="ISB17_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAP1980698.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1008..1184)
                     /locus_tag="ISB17_002790"
     CDS             complement(1008..1184)
                     /locus_tag="ISB17_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980699.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            1480..2136
                     /gene="uvrY"
                     /locus_tag="ISB17_002791"
     CDS             1480..2136
                     /gene="uvrY"
                     /locus_tag="ISB17_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAP1980700.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            2133..3965
                     /gene="uvrC"
                     /locus_tag="ISB17_002792"
     CDS             2133..3965
                     /gene="uvrC"
                     /locus_tag="ISB17_002792"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP1980701.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            4022..4570
                     /gene="pgsA"
                     /locus_tag="ISB17_002793"
     CDS             4022..4570
                     /gene="pgsA"
                     /locus_tag="ISB17_002793"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP1980702.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            4722..4797
                     /locus_tag="ISB17_002794"
     tRNA            4722..4797
                     /locus_tag="ISB17_002794"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4755..4757,aa:Gly,seq:gcc)
     gene            4851..4924
                     /locus_tag="ISB17_002795"
     tRNA            4851..4924
                     /locus_tag="ISB17_002795"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4883..4885,aa:Cys,seq:gca)
     gene            4937..5023
                     /locus_tag="ISB17_002796"
     tRNA            4937..5023
                     /locus_tag="ISB17_002796"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4971..4973,aa:Leu,seq:taa)
     gene            complement(5154..6170)
                     /locus_tag="ISB17_002797"
     CDS             complement(5154..6170)
                     /locus_tag="ISB17_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1980703.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALTEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKEK
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKTGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(6139..6402)
                     /locus_tag="ISB17_002798"
     CDS             complement(6139..6402)
                     /locus_tag="ISB17_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAP1980704.1"
                     /translation="MKSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFMNPIKFRNLQEVTTRKDDEPDFGAIFNGRKEKEPSR"
     gene            complement(6612..6794)
                     /locus_tag="ISB17_002799"
     CDS             complement(6612..6794)
                     /locus_tag="ISB17_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980705.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFTPQELITPNQK"
     gene            complement(6794..7363)
                     /locus_tag="ISB17_002800"
     CDS             complement(6794..7363)
                     /locus_tag="ISB17_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP1980706.1"
                     /translation="MSTAIAIAKEYASLIDLLETADELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNANTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRIR"
     gene            complement(7360..9576)
                     /locus_tag="ISB17_002801"
     CDS             complement(7360..9576)
                     /locus_tag="ISB17_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980707.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIAAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKIWEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVDSDCSSA
                     DISQVSDLMKLPARERFAAVLLFSHDANEVDSELLVQTREYLEMLDNSDTDSEDEVDA
                     FNRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPG
                     KRDEMLPEVKPETASARPYKQTHATLDREIACALLPVAPEKITPSILKAADEIISQDR
                     EDFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRD
                     ANAVEEKPKEAAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLR
                     EQFVTPRHVYDVPENNAVSQREPAAITLAEETPPQEQLSEQVKDLVQNVDALVERIHA
                     EEHKRQNAISAIETELKDSDDTDNLALWKNVFKTDERFTSAFSQNGGGTSINGTYIAM
                     KATREFGPFGIGWGVEVLEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTM
                     KVRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSAD
                     IFMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTANEV
                     NKVFGLIAREVDVHRKAAETKGDKEYSSYLGSRLRRITDIKTERLAALTAAQEQTA"
     gene            complement(9607..9927)
                     /locus_tag="ISB17_002802"
     CDS             complement(9607..9927)
                     /locus_tag="ISB17_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980708.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLLVATVDMSKNFPDRYILLES
                     REIEITVNQPEPIDIIGLQVEQLREQKQKTVADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            complement(10938..11351)
                     /locus_tag="ISB17_002803"
     CDS             complement(10938..11351)
                     /locus_tag="ISB17_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1980709.1"
                     /translation="MNTKTKNMAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCN
                     GVTPRKDKLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRG
                     LPESEKNKLINELKEKREHFDLLLKELLEAKNQTK"
     gene            11450..11680
                     /locus_tag="ISB17_002804"
     CDS             11450..11680
                     /locus_tag="ISB17_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1980710.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTEAA"
     gene            11739..12215
                     /locus_tag="ISB17_002805"
     CDS             11739..12215
                     /locus_tag="ISB17_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980711.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPELTIQEIDEAIAALVATRGAVIRTAQDHHA"
     gene            12255..12479
                     /locus_tag="ISB17_002806"
     CDS             12255..12479
                     /locus_tag="ISB17_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980712.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            12476..13231
                     /locus_tag="ISB17_002807"
     CDS             12476..13231
                     /locus_tag="ISB17_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1980713.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGVLLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEIITDGESADADTQQPADQRINYQV
                     ILDTYHEILPEMPPVKILTDGRKKHLRTFWKKFNFNESRWRAYLEFIGGNCRWMLEDR
                     PNGRGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            13221..14636
                     /locus_tag="ISB17_002808"
     CDS             13221..14636
                     /locus_tag="ISB17_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1980714.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIKGLFQADKPTDLITLTELMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMIQPADMTATDRITAVQQVIGQVAEHSRTGHKGG
                     LRPASEVVDDWIDDLERRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPNLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRLT
                     NDRSEKNKRYSRSDNSRTNEF"
     gene            14675..15085
                     /locus_tag="ISB17_002809"
     CDS             14675..15085
                     /locus_tag="ISB17_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980715.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAKPARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            15087..15323
                     /locus_tag="ISB17_002810"
     CDS             15087..15323
                     /locus_tag="ISB17_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980716.1"
                     /translation="MAGQSDYLPPGLPHNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYREFFRERLNYWRGVMEQ"
     gene            15320..15631
                     /locus_tag="ISB17_002811"
     CDS             15320..15631
                     /locus_tag="ISB17_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1980717.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSVKYLQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            15628..15852
                     /locus_tag="ISB17_002812"
     CDS             15628..15852
                     /locus_tag="ISB17_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980718.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            16534..17322
                     /locus_tag="ISB17_002813"
     CDS             16534..17322
                     /locus_tag="ISB17_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAP1980719.1"
                     /translation="MTVQISAESLSPLHHNQLPVITTELLASLYGTEVNGIQQNFKRN
                     IARFVVGKHFFKLEGNELRDFKNRLTDSQSVAKHTRSLILWTERGAARHAKMLETEQA
                     WEVFEKLEDCYFSQRPERRSTTTDDRTPLRDAVNILVGKKGMMYPDAYTMIHQRFAVS
                     HIEQLSQTQMLEAIEYIHCLLLDENHPTIPDFSFITTIKNGKVTGMRHVAKGEHLMTF
                     DAFKEIAERAGYLVIHSDNLRSMTLDKLMVMGKKSAISVANSAF"
     gene            complement(17497..18420)
                     /locus_tag="ISB17_002814"
     CDS             complement(17497..18420)
                     /locus_tag="ISB17_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAP1980720.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDEIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLSANE
                     YVQLSDAYRKNFNRNLDKQMESLFLENNANAFSKRITYTVQATDDQKNVFVKTSTDNP
                     ISVGAALLTSLSIITPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQAKYEAEKQIER
                     QAQLQPQKADNLDQSRMAFANADSDLSTVWSKLTPAKKKDLLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
BASE COUNT         4663 a         4371 c         4788 g         5069 t
ORIGIN      
        1 tttactatgc aggatacgga ttttttcagc tggcttcgca cgatgctgtt gcgttttcag
       61 aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca gcagctggag
      121 tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc taaagtggct
      181 ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa ttttctcgca
      241 attgatccgg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg gaatgatgac
      301 ttattcagcg aagcacagcc gttatgggaa gctgcgcgcg cacatggttt acgccgcggt
      361 gtcactcagt atttaatgct gccaaatcgg gcgttgggct ttttgtcctt ttcccgttgc
      421 agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca gctactggtg
      481 cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac gccagagatg
      541 aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaaggtaa gacatcagca
      601 gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca gaaaaacatg
      661 cagaaaaaaa tcaatgcacc gaataagacg caggttgcct gttacgcggc cgctactggc
      721 ttaatttgat ctcttttctg ttctgcgtgc cagatgcaaa aaccggctga aaggcacgct
      781 ctcagccggt tttatattac tgacggtagg cttgtttaat ttgcttaacc gttttggaaa
      841 atactgcagc ctgggtttcg tcttcgatca gcgcaagctg tttttccatc tttaacatca
      901 cgcggcccgc gtcagcttgt cccatcgcct gcagcatcag cgtcagcatc gctttcaggc
      961 aggagacttc atttgccagt tcttgattat tctcggcagt agaaaaatca ggcgtactca
     1021 tttattttcc tcgtcatgtt gcaatgaaaa tttgcggtga aaaatgttaa ggcggcggag
     1081 tataccataa gctttgctaa aaatagcagt ggttgttttt tgagcgtgat atcaacagta
     1141 ctataaaata cgttaatata atgaccaata aatattttta tcatgaatgt tttttgcccg
     1201 tattgttcgg gttaattaat gttacatatt cagcgggctg attttcattt ttgctgaata
     1261 aagtcaattt ttgtcacatt tcatcgccgg gcttactgtg aaacgatccc ggaagccgtt
     1321 ggtgacgggc gtgaccataa ctgtggacaa tcgaattgac aaaaacgaga gaaaaatcga
     1381 atacccacca tttttaacgt ttcaaagttg caataaaaac cgctaatata cgaatgacta
     1441 actatcagta gcgttatccc tatttctgga gatattcctt tgatcaacgt tctacttgtt
     1501 gatgaccacg aactggtgcg cgcagggata cgacgcattc tggaagatat aaagggtata
     1561 aaagtcgtcg gtgaggcatc gtgcggtgaa gacgccgtta aatggtgccg ggcaaatgcc
     1621 gttgacgtgg tgctaatgga catgagtatg ccgggcattg gcggtcttga ggcgacgcgt
     1681 aaaatcgcgc gttccacagc tgatgtcaaa atcatcatgc ttaccgtcca tacagaaaac
     1741 cctttaccag cgaaagtcat gcaggccgga gctgcgggct acctcagcaa aggcgcggct
     1801 ccgcaggaag tcgtgagtgc gattcgttct gtctattcag ggcagcgtta cattgcttct
     1861 gatatcgctc aacaaatggc gttaagccag atcgaaccag aaaaaacaga aagcccattt
     1921 gccagtttgt ctgaacgtga attgcagatt atgctgatga tcaccaaggg ccagaaggtc
     1981 aatgagatct cagaacagct caatctcagt ccgaaaacgg tgaacagcta ccgctatcgt
     2041 atgttcagta aactaaacat tcatggcgat gttgagctga ctcacctggc aattcgccat
     2101 ggtctgtgta atgcggagac attatcaagt cagtgagtga tcagtttgac gcaaaagcgt
     2161 ttttaaaaac cgtaaccagc cagccaggcg tttatcgcat gtatgatgct ggtggtacgg
     2221 ttatctatgt cggcaaagcg aaagacctga aaaaacggct ttccagctat ttccgtagca
     2281 acctcgcttc gcgcaaaacc gaagcgctgg tcgcccagat ccagcaaatt gatgtaacgg
     2341 ttactcatac agaaaccgaa gcgctgttac tggaacacaa ctacatcaaa ctttatcagc
     2401 cgcgttacaa cgttttgcta cgcgatgata aatcatatcc ttttatcttc ctgagtggcg
     2461 atacccaccc gcgtctggcg atgcatcgtg gagcgaagca tgccaaaggt gaatatttcg
     2521 gcccgttccc gaatggctat gccgtacgtg aaacactggc gctactgcaa aagattttcc
     2581 ccattcgcca gtgcgaaaac agtgtttatc gcaaccgctc gcgtccgtgt ctgcaatatc
     2641 agatagggcg ctgtctgggg ccgtgcgttg aaggactggt gagtgaagaa gaatacgctc
     2701 agcaggtcga gtatgtgcgc ctgtttttgt ctggcaaaga tgatcaggtg cttacgcaac
     2761 tcattagtcg tatggaaact gccagccaga atctggagtt tgaagaagcg gcgcggattc
     2821 gcgaccaaat tcaggcggtg cgacgtgtca ccgaaaaaca gtttgtttcc aataccggcg
     2881 acgaccttga cgttattgga gtggcgttcg atgcgggtat ggcttgtgtc cacgtattgt
     2941 tcattcgtca gggcaaagtg ctcggcagcc gcagctattt cccgaaagtg cctggcggta
     3001 cggaactgag cgaggtggtg gaaaccttcg taggtcagtt ctatttacaa ggcagccaga
     3061 tgcgcacctt accgggtgag atcctgctcg attttaatct tagcgataaa acgctgctcg
     3121 ccgattccct ttcagaactg gcgggacgca agattaatgt tcaaaccaaa cctcgtggcg
     3181 atagagcgcg ttatctgaaa ctcgcgcgta ccaatgcggc gacggcctta accagcaaac
     3241 tttcgcagca atctaccgtt caccagcggc tgaccgcgct tgccagcgtg ttgaaattgc
     3301 cggaagtgaa gcggatggag tgctttgaca tcagccatac catgggtgaa caaaccgtcg
     3361 cttcctgtgt ggtgtttgat gccaacggcc cgctgcgtgc ggagtatcgg cgctataaca
     3421 ttactggcat cacgccgggc gatgattacg cggcgatgaa tcaggtgctg cgtcggcgtt
     3481 atggtaaagc tatcgacgac agtaagatcc cggatgtgat ccttattgac ggcggcaaag
     3541 gccagcttgc gcaggcgaaa aatgttttcg ccgaactgga tgtctcatgg gataaaaatc
     3601 atccgctgct acttggcgtt gccaaaggag cagatcgtaa ggctgggctg gaaacgctgt
     3661 tctttgagcc ggaaggtgag ggatttagtt tgccgccaga ttctcccgcg ctgcatgtta
     3721 tccagcatat tcgcgatgaa tcacacgatc acgcgattgg cgggcaccgt aaaaaacggg
     3781 cgaaggtcaa aaataccagt tccctggaaa ccattgaagg cgtcgggcca aaacgtcggc
     3841 aaatgttgtt gaaatatatg ggcggtttgc aaggtttacg taacgccagc gtcgaggaaa
     3901 ttgcaaaagt gccgggtatt tcgcaaggtc tggcagaaaa gatcttctgg tcgttgaaac
     3961 attgatgtct ctgtagcaac ataggggtaa tcttactgac aacagatagt tacccgtcat
     4021 tatgcaattt aatatcccta cgttgcttac actgttccgt gtcatcctta tcccattctt
     4081 tgtattggtc ttttatttgc ctgtcacctg gtcgccgttt gccgccgcgc tcattttctg
     4141 cgtcgcggcg gtgactgact ggttcgatgg ttttctggca cgccgctgga atcagagtac
     4201 ccggtttggt gctttccttg accctgtcgc agataaagtt ctcgtggcta tcgccatggt
     4261 gctggtaacc gagcattacc acagctggtg ggtgacctta ccggcagcaa cgatgatcgc
     4321 ccgtgaaatt attatttctg cgctacgcga atggatggcg gagttgggta aacgcagtag
     4381 cgtggctgtc tcctggattg gaaaagtgaa aaccactgcc cagatggtgg cgttggcatg
     4441 gctgctgtgg cgtccgaata tttgggttga gtacgccggt attgcacttt tctttgtggc
     4501 tgcggtactg actctgtggt caatgttgca atatttgagc gctgcgcgtg cagatttgct
     4561 tgatcagtga tcgtttcggc gcaattttca gcaaacgatc aaaagtggtg aaaaatatcg
     4621 ttgactcatc gcgccaggta agtagaatgc aacgcatcga acggcggcac tgattgccag
     4681 acgataataa aatcaagtga ttaactgatt gcttgatgaa tgcgggaata gctcagttgg
     4741 tagagcacga ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccagtt
     4801 taaaaggcat cggcgtcagc ggatgtctgg ctgaaaggcc tgaagaattt ggcgcgttaa
     4861 caaagcggtt atgtagcgga ttgcaaatcc gtctagtccg gttcgactcc ggaacgcgcc
     4921 tccacttttt tcccgagccc ggatggtgga atcggtagac acaagggatt taaaatccct
     4981 cggcgttcgc gctgtgcggg ttcaagtccc gctccgggta ccatgggaaa gataagaata
     5041 aaatcaaagc aataagcagt gtcgtgaaac caccttcggg tggttttttt gtatcttctt
     5101 tctcatttcc gaagatgcgt ccgaagatga tttttctctg gacgcatcat cattcatttt
     5161 ctctggccac caactacggg cactacttta actttccggt catatgctgc tgtctgtcgc
     5221 gggttcttat gcccggaaat tgcttgcttc tcttccaggc tgccttccag atcagagata
     5281 ccttttgctt tcagatcatg aaatgtgaag tctatttgca gatgaggata tttttcctgt
     5341 gctgccgctt tgacatcacg ccaacgagag ttaaaaccat cacgggtata cttgccaccg
     5401 gtagtttgat gaatcacaaa caggctactg attcccgttt ttaatggcag agaacgagcc
     5461 agagctatcg ctttctgcag acgaggcgac caggctttga tttgcttaac gccggttttt
     5521 ccctgacgaa tgtagatccc ggaatcgaac agctggtctt tctgcaatga aagtacatca
     5581 ctctgtctgg ccacgcataa gtaggcaatc tccattgcta cgcgaacaac atcaggagac
     5641 acttcataaa cggcctgata ttcttcatcc gtaatataac gttcgcgagc tttctcttta
     5701 aactgtttaa ctccctgaca aggattcctc tgaacataac ctcgttcata accccaacgg
     5761 aatacccggg acataaagct tttttccctg tttgcctgag ttcggcttga taaaccgcgt
     5821 tgatccatat agcgccgaat atgttcaggt ttgattttat ccgggtcgat cttcccgaag
     5881 acaggcagaa cttttccgga atattttgtg tagtctttcc gggtttcgac tgccaaatcc
     5941 ataaaatcag gggaggtcat gaattgttcc gtgagcgcct gaaaagtatt tctttctttt
     6001 tcgtcaccga ccgctttttc atatgccagc catacggctg atcgtggtgc atccaatgcg
     6061 cacaggcgta tcgctttgtt atctttattc ctgaattcgt aggccgcttt gccctggtaa
     6121 acacgaggcg gcatccagtt atctgctggg ttctttctct ttccggccat taaatattgc
     6181 tccaaaatca ggttcatcat cttttcgcgt cgtcacctcc tgtaaattgc gaaattttat
     6241 tgggttcatg aaatggcccc aggttgtttt tgggtgtccg tcagcccttt ccatgaagaa
     6301 aattccagcc cggcgaagag cctcacattg ctttgattta agtgggcttc ccgtcagctc
     6361 gaccatttct tctctggtga ttatgtcgtg atcgcttttc atggtctttc ctcattatgt
     6421 agcttattgc gtcatctgcc ttctggcagg cacgggcaat atcagactcg gtcaaagtct
     6481 gtttcctgac gctggccgac agtcgaccaa ttttgatatc aaatgcggag agcagagttg
     6541 ctcccggttg ccatcgcagc attgtgatcc tccggtgtct ggtgaatcgc aatgctagcg
     6601 atggtgagtt tttatttctg attaggcgta atcagttctt gtggggtgaa tgccgatttc
     6661 tcccgagtga ccttaatgtt ttctggcagg tgtagcccaa gttcgcagcg gctccgtgct
     6721 tcaataatgc cattggtacc atcggcaagt accaggtgaa ccgcgtcacc acgctttaat
     6781 gtgagtttaa gcattagcgt atcctcagtg accgttcgcc gcgttccaga tgtgcgcctg
     6841 gtaccgggtt taataactcg gctggtggtg tttctccacg cgccagtatt tcggcggcaa
     6901 ccgcctccgc agattcaatc acttctttaa tggctcgctt gtttggtgta ataaccgttt
     6961 ctacatcaac aagtgacacg ccctcatact catccgggat cttatctttg ctatcgataa
     7021 ttacccggat agccccctgt gcatcagtaa atgtgttttt tgcggttttc agtttatcca
     7081 ggccagcagc ttgcaggcag gtgagcatat gctttcgaat tgctttttcc ttgccttcaa
     7141 aggacgtctt acgggttgcc aggcgagaca tttcttcagc gcaggtattg gcattgccct
     7201 gtaaattacg gcagatgacc atcatggcgt ccagtttgtc tcccagttct ccttccatgc
     7261 cttcaagcgt gtcggcgatc atctctggcg tcagttcatc ggcggtttct aacaggtcaa
     7321 tcagactggc atattctttt gcaatagcga tagcagtgct catgctgttt gctcctgtgc
     7381 agcggtaagg gcggcgaggc gttcagtttt aatgtcggtg atacggcgca gacgtgatcc
     7441 cagatacgat gaatattctt tatcgccttt ggtttccgct gctttcctgt gtacatccac
     7501 ttcgcgggcg atgaggccaa ataccttgtt tacttcgttg gcggttaccg cggcggccag
     7561 cgtattggca acgttgataa gcttctcatc cagttcctta cgcagacggg tagcatcttc
     7621 cgcattctcg ctggcattct tgatatcaaa ctcagcttta ttcctctggc ggtattccgg
     7681 attgtcgtac aggcccatga aaatatcggc gctgaatccg agaggggata gcgctttctt
     7741 aacagcatcc gtccaggatt tcttcggtgc ttcgccgtcg cttgttggtc cgtatttagt
     7801 gtcgtagata taaggtgtgc atccgtaagc ctcggactca ccgcgtaccc cattcaatat
     7861 gtaccagact ctcactttca tggtgtggtg catttcacat aggtacccgc caacaccgtc
     7921 ttgaatcaga tcccattcta ttttttcacc aacctgtttt ttgcgaacta ttggtgcgcc
     7981 tttatcaaag cgctcctcca gcacctctac cccccagcca ataccaaatg gcccaaattc
     8041 gcgagtcgct ttcatcgcta tataagtgcc gttgatggat gtgccgccgc cattctgaga
     8101 gaaagcactg gtgaagcgct catcggtttt gaatacgttc ttccacaatg ccaggttatc
     8161 cgtgtcatct gagtccttta actccgtttc gatagctgag attgcattct gccgcttgtg
     8221 ctcttcggca tggatccgct caaccagcgc gtcaacgttc tgcaccagat cctttacctg
     8281 ctcgctaagt tgttcttgtg gaggagtctc ttctgccagg gtaatagctg caggttccct
     8341 ctgtgatacg gcgttatttt caggtacgtc atacacatgg cgaggcgtga caaactgctc
     8401 acgcaactgc tgcaaactac gttctccagc atttgcagct tcgctttttt cgccctgatt
     8461 tgagggtgtt tctgacatca tcccatcaat tgagaatcgt ccgccgccgt ggttggtgac
     8521 ttttacatca tccttgggtg ctgcttcctt tggcttctct tcaacagcat ttgcatcacg
     8581 ctgcccgtta gcttgcagcc agttgtcgat gtgactgcgc agggattgtg ggaagtggta
     8641 cgtgtctttt gcgggggcgc tctgaataac accgaatact gatgcgcggt cataggcaag
     8701 gatctgatcc gttgtgcgca aggccattga ccagcgttta aaatcttccc tgtcctgact
     8761 gattatttcg tctgccgctt tcaggatgct gggggtgatt ttttccgggg caacaggtaa
     8821 cagggcgcag gcaatttcac gatccagagt ggcgtgagtc tgtttgtaag gacgtgcgga
     8881 tgctgtttct ggttttacct ccggcagcat ttcatctcgt ttaccgggat tctctaccca
     8941 tttttttata aatttggaga tagtccaact ggtcgggttt tgttcctggg tatcgcaact
     9001 tgccaaaatt gcatgtacca gattattcaa tccagcaata tgcatgtact cgatgggctt
     9061 acacgccacc atggcatcaa gaacgatgcg gttaaacgcg tcaacctcat cctcactgtc
     9121 agtatcactg ttatcgagca tctccaggta ttcacgcgtc tgcacaagca attcactgtc
     9181 gacttcgttg gcgtcgtggc tgaacagcag aacggcggcg aatcgttcac gagccggcag
     9241 tttcatcagg tcgctaacct gtgagatatc tgctgaactg caatctgaat caacaggagt
     9301 gttaactacc catttttcgc catcaaaact gtgttctgtg gcaaacgtct catcaaactc
     9361 accgacgcct gggcgcggtt ggcccggggc atcttcccag attttaggtt tgaaatagtt
     9421 gtcgccgtgg tcagggtagg cttcccacaa tttgccgatg atgatgttct cggcaacttt
     9481 tttgttaggg gcggcgatgg cgatggccag cgccgggatc gcgccgtttt tgagcgcgcc
     9541 ttttttcgct tctaacaagc cgttaaaaat ggtcattggt ctttcctcat tacaggtcag
     9601 tggtttttag taggggagct catcggtatc tggcgtgtat tcgatgcaaa gtaactgctg
     9661 gattttgtca tcgatagcag caatacgctg ttgggcgtcg gcgactgttt tctgtttttg
     9721 ttcacgcagt tgctcgacct gtaaaccgat gatgtcgatt ggctcgggct ggttaacggt
     9781 aatttcaatt tcgcggcttt ccagaaggat gtacctgtct ggaaaatttt ttgacatatc
     9841 aacagtcgca acaagcagct tttcgtggct gaaagttgaa gttgcataat gaataaagag
     9901 tgttactggg atggtgcgcg cttccatagc gactccttgg taatgtatac tcagagccga
     9961 tcagcgaata ttgatcagct tccatagcgt cagtgtcgtt ggtctttcct catcacaagt
    10021 ttggtcactt gtgataaatc cgaatggttt ggtcgccgtt cggggtaact ggcccgcctt
    10081 gtgcgggtct tttgctatct aaagggtgcc ggttacgttt ccggcgtcgt tatgtttcca
    10141 ttacgaccgt acagggctgg tcaggcccgg gctggtcttt cctgttttga gctggtcagg
    10201 ctcatggtca acgctggtca ggcgtggtac ttcctccggt ctttccctgg tgtcacgctg
    10261 cgggtttggc ctctcctgtt ggggactcag gcacagcgat aaaacctggc tgtgtgaaaa
    10321 aatggcccat cgtgcggact gggcaaagac taaacacagc aaattgatga tgggcgcccg
    10381 gaatcgaacc gggtaacggg cagggagttc ccgttaaaca cctgttcacc acaaccggga
    10441 gcgcactccg ccatttcaaa atttaacgac aaagctcaaa gttgagtcga tgaagtgcgc
    10501 tctcgtgttg tagccaggac tcttccctgg tggtcacacc gtatcgcctt gatggtgaat
    10561 caatcgctca tacctggctg tggcttgcac attccggcta cctgctatgg agacaacgcg
    10621 ttaaggctcc gcttccatcc agaccgcttc gacacatgtg ccatatgccg gtgaagtctt
    10681 tccctctgtc accgtgttgg cgccgacgcg caaaatttgg tgccgtcttt ccggctgtca
    10741 gaacgtgttt ctgaacaact gccgctgggt aagcgttgtt gatgaaagtg actttacaat
    10801 ttgtatttgt aatggtcaat gtgtttttaa aaaataattt gtagtgaggg gcaaaaaaat
    10861 attcactgtg taaaggtgtt tttgtgttag gggaaagttg catgccagca gcggctggcc
    10921 tggaaatttg aactgaacta ttttgtctgg tttttggctt caagtagttc ttttagcagt
    10981 agatcgaagt gctctctttt ttcttttagc tcattgatta atttattttt ttcgctctca
    11041 ggcagtcctc tgaatagttg cagtagagcc tgttcttgcg gggttagttc ttgattgtaa
    11101 ttacccgggt gtagtgtgga ttcatccaaa tattcccctt cattcatgaa gaaaaaatga
    11161 acagggtatc cggtcacttt tgctagcttg tctaatttat cttttcttgg agttacccca
    11221 ttacaccatg cctgaactga ttgagccgta accccaatat ggcgagctaa ctcagattga
    11281 gaccagccta agtcttttag aatccgttgt agtcggctag caaaagccat gtttttcgtt
    11341 tttgtgttca taccgtaagg atacaaggtt taattgtagg gagcattgca aatataattt
    11401 gtaattacag ttattgtttg taatgatgac gcggacacaa ggagtccata tggataaaca
    11461 actgcaagac aaaattcttt ccgtaatgag tcaatccgag ttagggcggc gattagggaa
    11521 aaaaccacaa acggtaagcc tctggttcaa aggacgggtt cccggtgaag aggttttgcg
    11581 cacatctgaa gctcttgaat ggcgcgtgac ccctcatgat cttagaccgg atctttaccc
    11641 aaacccagat gacagcttgc ctaaaacgga agcagcttaa ctgttggtga gccccaaatc
    11701 tgattaagcg taatcaattt ttagcgacag gagacgctat ggaaaaccaa gaggaattac
    11761 aaaaagagat tttgacctgg gcggcaaggg cagggcagga actcgtcacg attgaaatct
    11821 gccgggcctg gttcagtcag ggacgcaatg atgagttgag actacatgaa tttgaggacg
    11881 cggacggcaa cgtggactgg agagccatca acaacaatcg gcagaaaatc tttcgctggt
    11941 tacgtggcga gacaacggcg gcgcgccgaa aaactcaggt gctggccagt gtgatgaaag
    12001 ccgtgctacc cgcagaacgg cgggcacgtc tggagtcgcc gggcgatccc gttttgctgg
    12061 caacgctggc ggcaaaagaa ggggtggaag cgattaacgc tgtacatctc cacttcgcgc
    12121 cagagttaac tattcaggaa attgacgaag caatagcggc gctggtagcg acgcgaggag
    12181 cagtgatacg cactgcgcaa gaccaccatg catgagcgcc tgaccagcgt ttaacttata
    12241 ccgaggaaag accgatgtta agacatattg accgcattac ctggcgtaac ggctggcacc
    12301 tgaatggacg cccggcacat gttgcagaga tccgccccat attcgatggc cgcgtcgcag
    12361 ctgcacgttc tgtatgggaa aagtacgaag aagaaaaagc gaaactgcgt gagcaaaatc
    12421 tctctggcgc tgcctatgag gctggctgtc gcgttctctc agaggctttg ggcatatgaa
    12481 catcctcccg ttacttgata gacccatagc cttccagaga agttttatcc gtcttgaaat
    12541 gggcgtgact gccgcattgt ttctgtcgca gttgacgtac tggacaaaca gaacaactga
    12601 cgacggctgg gtatataaaa cccaggatga gtgggaagag gaaacagggc tttcacgata
    12661 cgagcaggag ggcgcgcgca aaaaactacg tggtctcggc gtcttgctgg aacgtaaaca
    12721 gggcttgcct gccaggctgt actacaaaat cgataatgat gtgttgtgcc agctacttac
    12781 gtccgcatac aaggatgcgg aaaaaccaca tacaggtgag gggaaaacca ccaggcctgt
    12841 acgtggaaaa ccaacaaaca ttcttacaga gaatactaca gagattatta ctgatggtga
    12901 atcggctgac gccgacaccc aacaacctgc cgaccagaga ataaattatc aggtgatact
    12961 cgatacctat cacgagattt tgccagagat gccaccagtg aaaatcctca ctgacggcag
    13021 aaaaaaacac cttcgcactt tctggaaaaa attcaatttc aatgagtccc gttggcgggc
    13081 ctatctggaa tttatcggtg gcaattgtcg ctggatgctg gaggacaggc caaatggcag
    13141 gggaggattc tggcggcgta aaaatcttga ttatctgatt accgaacgct gctatgtttc
    13201 ggtcaaggaa gaacgcgcaa atgataaata acgcgattgc actgtacagc gttgacgcag
    13261 aacaggctgt tctgggctgt ctgatgctga ataccgacca cgacagaacc aatgcggtat
    13321 ttgcgttact gaaaccggaa acgttttata tcaatgcgca tcaggttatc taccgggaga
    13381 tcaaaggatt atttcaagct gataaaccaa ctgacctgat aacactgacc gaactgatgg
    13441 aatcgaaggg gctgtatgag cgcgtggggg gattcgcata cctggctgaa atgagtaagg
    13501 cggcgattcc ggcctcaatg gtcaactacg cgaaaattgt acgtgaaaaa gccattcttc
    13561 gttatgccgt ggaaaagttg aattcttgtc tggagattat gatccagcct gcagatatga
    13621 ctgctactga caggatcacc gccgttcagc aagtgattgg gcaggtggcc gaacattccc
    13681 gtaccgggca taagggcggt ttgcgtccgg caagtgaagt cgtcgatgac tggatagatg
    13741 accttgagcg ccgtttcaat gatccagcac atgcagccgg actaacgctc ggaatcgaaa
    13801 gccttgatcg cttaatggca ccaaagcagg cgttaagagg ttcattggtc gttattggtg
    13861 cccggccaaa aatgggcaag acagccgcgt ttaataaaat tgcggtgcat tttgctctga
    13921 atcatcgttt gccgacgctg gtatttagcc tggaaatgac tgacagaagt ctcattgaac
    13981 gcatggttgc gcaggaagca aaggtcaatt cggagatttt ttatcttggc cctaacgatg
    14041 aatctgaatt gagtctcgca atagcaaagg cgggggagat tgccgaatcc aacatgatga
    14101 ttgacagcac ggcaggtgta acccttgcgc atattgtcgc tgaatgtcga aaagttaaac
    14161 ggatgcgtgg ctcggttggc ctggtggcaa ttgactattt gacgctgatg aagaccgaat
    14221 cggcagagcg tagggatatt gcgtatggcg atatcacaac cggtttgaag aacctggcaa
    14281 aagagctgga ttgtgttgtg gtcctgctga cgcaactcaa ccgaaagctt gaagaacgtg
    14341 ccgataagcg gcctaacccg agcgacagta aagataccgg gcagatcgaa caagattgcg
    14401 atgtctggat tggcctctat cgtgatgctg tttataacga aaatgccgac ccaaatctca
    14461 tggaaatgtt gctacgtctg aaccgcgaag gaccatcggg aacagcctat gccctgatga
    14521 aaaatggctc agttattgat atcagcgatg aggaggttta tcgccttacc aacgaccgct
    14581 cagaaaaaaa taaacgttat tcccgtagcg ataattctcg cacaaatgaa ttttaaccac
    14641 gcctgaccag cgtgaaataa ccgaggaaag accaatgacc accactccag gcaaattaga
    14701 gtacccgtcg gacaaaggac acatagacga cggtaaaaac tatctcgacg tcattttgtg
    14761 gaatatgaac gtcgggccac gtgcgcgcac tcgcgcggtt tttgtgccaa gacctaaagc
    14821 aggcaatttt tcaacccctg ctaagcctgc ccgtcaggca tccgtagccg cagcgccagc
    14881 agtgaaacgt aagggcaaaa cgcacaccgg gatcgcgatt cgcagaaatg gtgagcggca
    14941 agtgaaactc cacgaaacag cgacaacctg gtgtgcctca ccgcatgaaa cttacgacaa
    15001 gataaccggg cagcgcattg gcgcgcctgg tcgctgccgc ctgctgctga gttccatcac
    15061 tccgatagca aagaaagggg cgtgatatgg ccgggcaatc agattacctg ccgcccggct
    15121 taccacacaa ccgcgccaaa tggccccagg aataccagct taaagagcac tacgacatgc
    15181 gagcggcggc gctgatccgc caactctttg agaaacgtat tccgagggga agcgtgattg
    15241 agcaaatcgg aatgacgcca gatacgtacc gggaattttt cagagagcgt ctgaactact
    15301 ggagaggggt gatggaacaa tgaaatacaa acggtgggtg cgtgcggaag tcgtaatcat
    15361 caaacaatgt gcgggcagca tgacagttga acgtataggg cagcttattg gcagaaccgg
    15421 tgcagcggta cgcacaaaag cgcgcgagct gaaaatctgt atgtatctgc ggggtaatta
    15481 tcaccagtca gtgaaatacc tccaggaaga tatcgagctg gcaagggaat tacatcagtc
    15541 gggtattaat cgccaggata tcgcagaaaa actcgaaatg ccgatcggag cggtgaatca
    15601 gtttgtttat tttgagcgga gaatttcatg aaagaacttt ttcttgcatt tgtgccccgg
    15661 tttattaatg accagatcgc actgactgat aatggtgaac aatatgaaat tgcctgcagc
    15721 atggtggatg tgaatcctgg cgaacggtat gacgcgatgt gtgacctgaa aatatttacc
    15781 tggctgggtt gggctattcc gtgtggagaa ccaaccaata ttcgtccgtt tgagagcagg
    15841 gaggctgtat gagtgaattg aaaatacgtc gcaaggcaat agcacgcgtt aaaaatctat
    15901 taccagtgcc tgtcaaatat cgtttttgtg gtgggaatgt acggattggt acggatgtat
    15961 ggtgattgcg gagtgacgta aaacgaatcg ttgtgggtgt aatatcgtca atatcattgg
    16021 tttcccggag ggaaaggaca tgtttggcga taaactggag ttaccacgga acgggcggat
    16081 agtgctctat atttcagatt acaaagtaac ccgggctgag gtgttggaac cggaaaaaca
    16141 tctggtcacg ttaccggaca ttattgagtt gtataagcgg gtcggatatg tggacttgca
    16201 tcgcgatgat gttcccgttg agtgtatgga atgtgaacac cggaggtttg aagttgattg
    16261 aattgactta gaaaaagtcc ggggctatag tttccgcgca gccgcaaaat cggttgccgg
    16321 gattggcgtc ccggatatct aagtgacgca tagccgcgtt agcggttttt ttatgtgtta
    16381 ggcacggcca cattcgcatt atggtgggct gtgtgggggc ttctgtggaa gcgccgggtt
    16441 cacttagccg gttacgccaa ccctgcacag ctcaccacca gatgattggc gtcgctggtg
    16501 gtgatgaaaa aaacactaag tgaggccact gcgatgacag tccaaatatc tgcggaatcc
    16561 ctttctccgc tacatcataa ccaacttccc gtcatcacta ctgaattgct tgccagtttg
    16621 tatggcacgg aagtaaatgg tatccagcaa aacttcaagc gcaacatcgc cagatttgtc
    16681 gtaggaaagc atttttttaa gctggaaggt aatgaattac gcgacttcaa aaacagactg
    16741 acagatagtc agtcagttgc taaacatacc cgatccctca ttctctggac agaacgcggc
    16801 gcagctcgcc atgccaaaat gctggaaaca gaacaggcct gggaagtttt cgaaaagctg
    16861 gaagattgtt attttagcca gcgtccagag aggcggagta caactaccga tgaccgtact
    16921 cccttgcgtg atgccgttaa tatactggtc ggcaagaaag gcatgatgta tcccgatgcc
    16981 tacaccatga ttcatcaacg cttcgccgtc tctcacatag aacaactgtc acagacgcaa
    17041 atgttagagg cgattgaata catccactgc ctgctactcg atgaaaatca cccaaccatt
    17101 ccagattttt cttttatcac cactatcaaa aatggaaaag tgaccgggat gcgccatgtt
    17161 gccaaaggcg aacatctgat gacctttgac gctttcaaag agatcgctga gcgggcaggg
    17221 tatctggtga tccacagcga caatctgcga agtatgacac tggataaatt gatggtaatg
    17281 gggaaaaagt cggctatatc cgttgctaat tcagcatttt gacaggttgt ttggggcgga
    17341 aatactctat ttgcgcttaa aaaggtattt gaccacgacc agcacgagac cgaatacctc
    17401 tgtcaaagta ccgcccatat aaaacagtta catatgcact tgatgactaa gctggcacca
    17461 ggttaacacc tggcgccagc ttgaaggaga cttcagttat tggtttctga ggaaagcgat
    17521 tcttgaaagg gtcatttgcg tctggcagtc gaccattttc ttgacttcag catctgttcc
    17581 ctgcattgaa actttaccgc acatagcatc cttagttttg atccactgac gctgagaagg
    17641 cagcaaatct tttttcttcg ccggggttaa cttgctccag acggtactca aatcagagtc
    17701 ggcattcgca aatgccattc gggactgatc aaggttgtca gctttttgcg gctgaagttg
    17761 tgcctgtctc tctatttgct tctccgcctc atacttagcc tgttcttgcg ccctgagttg
    17821 cacctgttgt tccgcttcaa tttgactctg ttgggcatcc ttagcctgct ggattttctg
    17881 ctgctcaacg atcggggtga taatggaaag cgatgtcagt aaagcggcgc ccactgatat
    17941 cggattatca gtagaggttt ttacaaaaac gtttttctga tcgtcggtcg cctgcacggt
    18001 gtaggtgata cgttttgaaa aggcgtttgc gttgttctcc aaaaacaggc tttccatttg
    18061 cttatcgaga ttacgattaa aattctttct gtaagcatct gaaagctgga cgtactcgtt
    18121 cgcagataga gtcatcgtca cggtcccttc acaagttttc attgtgctgc ccgtgtcact
    18181 tgatgttgtg gagatctcag agatggctag tttgatcttg tccagtgtgc ttcgtttggt
    18241 ctgattagtg acatcagggt atttatcgac ctgttcagag agtccttcat aagcagattt
    18301 ttttaataaa tccatgagag ctgactgggt tatttccgaa gaacaaccga tctcatcttt
    18361 tttgttatca cagcctgtaa gggcaatggc gagtaagagt actgagtatt ttaatctcat
    18421 gaattccctt tagagtaagg attggttttt aagacgcggt ctgtaatcag tcccgagagc
    18481 tacagtaaac aacgctgagc actgaacgca tcacaggtaa ggtaaaaggt gatatcggca
    18541 aaggctgtga aacctttaat tcaacagatg ggaacggtat gcgcaggatc ctgtaacgac
    18601 gctcccaaaa ccggaaacct gtaaggcatg ttttcgctgc tgaacttaat atacgaacct
    18661 gcactatcat gaacatattt tatatcgtta taccccgagt gattgtgccc ggcaatggat
    18721 agtgtcctgg aggaactatt tctactgtaa aaggtagata tttaatttca gttttgaatt
    18781 attccctgct gttaataaca atggctgtat atggcaccga gtaggcattt tttcgcttac
    18841 tgaagggcag ctgtgagcga ggaacagact ttccctactt cgttgagagt a
//
LOCUS       DACADA010000080        18754 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.081, whole genome shotgun sequence.
ACCESSION   DACADA010000080 DACADA010000000
VERSION     DACADA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.081"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            96..302
                     /locus_tag="ISB17_002815"
     CDS             96..302
                     /locus_tag="ISB17_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAP1980721.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            403..912
                     /locus_tag="ISB17_002816"
     CDS             403..912
                     /locus_tag="ISB17_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAP1980722.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            909..2327
                     /gene="phrB"
                     /locus_tag="ISB17_002817"
     CDS             909..2327
                     /gene="phrB"
                     /locus_tag="ISB17_002817"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAP1980723.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2369..3850)
                     /gene="dtpD"
                     /locus_tag="ISB17_002818"
     CDS             complement(2369..3850)
                     /gene="dtpD"
                     /locus_tag="ISB17_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAP1980724.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4121..4864
                     /gene="ybgI"
                     /locus_tag="ISB17_002819"
     CDS             4121..4864
                     /gene="ybgI"
                     /locus_tag="ISB17_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAP1980725.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4887..5543
                     /gene="pxpB"
                     /locus_tag="ISB17_002820"
     CDS             4887..5543
                     /gene="pxpB"
                     /locus_tag="ISB17_002820"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAP1980726.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5537..6469
                     /gene="pxpC"
                     /locus_tag="ISB17_002821"
     CDS             5537..6469
                     /gene="pxpC"
                     /locus_tag="ISB17_002821"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAP1980727.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6459..7193
                     /gene="pxpA"
                     /locus_tag="ISB17_002822"
     CDS             6459..7193
                     /gene="pxpA"
                     /locus_tag="ISB17_002822"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAP1980728.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7229..8020
                     /gene="nei"
                     /locus_tag="ISB17_002823"
     CDS             7229..8020
                     /gene="nei"
                     /locus_tag="ISB17_002823"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAP1980729.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8017..9063)
                     /locus_tag="ISB17_002824"
     CDS             complement(8017..9063)
                     /locus_tag="ISB17_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP1980730.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIIAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9243..9956)
                     /locus_tag="ISB17_002825"
     CDS             complement(9243..9956)
                     /locus_tag="ISB17_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1980731.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            10220..10906
                     /locus_tag="ISB17_002826"
     CDS             10220..10906
                     /locus_tag="ISB17_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HAP1980732.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            10919..11932
                     /locus_tag="ISB17_002827"
     CDS             10919..11932
                     /locus_tag="ISB17_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1980733.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            11944..12699
                     /locus_tag="ISB17_002828"
     CDS             11944..12699
                     /locus_tag="ISB17_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1980734.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            12714..13025
                     /locus_tag="ISB17_002829"
     CDS             12714..13025
                     /locus_tag="ISB17_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAP1980735.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            13025..14221
                     /locus_tag="ISB17_002830"
     CDS             13025..14221
                     /locus_tag="ISB17_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAP1980736.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            14249..15217
                     /locus_tag="ISB17_002831"
     CDS             14249..15217
                     /locus_tag="ISB17_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HAP1980737.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            15277..15738
                     /locus_tag="ISB17_002832"
     CDS             15277..15738
                     /locus_tag="ISB17_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1980738.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            15738..17048
                     /locus_tag="ISB17_002833"
     CDS             15738..17048
                     /locus_tag="ISB17_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP1980739.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
     gene            complement(17095..18378)
                     /gene="gltA"
                     /locus_tag="ISB17_002834"
     CDS             complement(17095..18378)
                     /gene="gltA"
                     /locus_tag="ISB17_002834"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAP1980740.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSDIKK"
     gene            complement(18584..18691)
                     /locus_tag="ISB17_002835"
     CDS             complement(18584..18691)
                     /locus_tag="ISB17_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980741.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT         4601 a         4408 c         5119 g         4626 t
ORIGIN      
        1 taaaattaca caaacagcgg tactattttc atcagataaa cgaattcatt tttccggtgc
       61 tattcaccgg gtagatacaa aagggggaga aaagtatgga actctacaaa gaatatcctg
      121 catggcttat ctttttacgc cgtacttgtg cggttgcagc gggcgttctg gcgctgcctt
      181 tcatgctttt ctggaaagac cgcgcccgct tttatagcta cctgcaccgc gtctggtcga
      241 aaaccagcga taaaccggtg tggatggatc aggccgaaaa ggcaaccggt gatttttact
      301 gattgccaaa catgacgttt aaagaaaacc gccggtggaa acgctggcgg ttttcttttt
      361 tcggcgtcta cacttactcc tgtaaaccgt tcaggagcag taatgaatca acaacttttt
      421 gatgacagca cccttatccg catttttgct ttgcatgagt tacacaatct caaagatcga
      481 ggactaacgc gcggggcgct tctcgattat cacagccgct ataaactcgt ctttctggcg
      541 cattctcagc cggagtaccg caaacttggc ccgtttgtgg ctgatattca ccggtggcaa
      601 agtctggatg actattacaa ccagtaccgc caacgcgtga ttgttttgct ttctcaccct
      661 gccaaccggc gtgatcatac taacgttttg atgcacgttc agggatattt tcgcccacat
      721 attgattcca cagaacgcca gcagctggcg gcgcttatcg acagttatcg ccgtggcgag
      781 caatcgcttc ttgcgccgct gatgcgtatc aaacactata tggcgcttta tcctgacgcc
      841 tggctttcag ggcagcgtta tttcgaactt tggccgcgtg tgattaactt gcgccatgca
      901 ggagttttat gactacccat ctggtctggt ttcgccagga tttacgtctg cacgataatc
      961 tcgcactggc tgccgcctgc cgcaattcgt ctgcacgcgt gctggcgttg tatatcgcta
     1021 ccccacgcca gtgggcggcg cataatgtgt cgccgcgtca ggctgaactt atcaatacac
     1081 aacttaatgc gctgcaaaac gcgctggcgg aaaaaggtat tcctttattg ttccgtgaag
     1141 tggatgactt tgccgccagc gtcgaaatag ttaaacaggt gtgcgcggaa aaccgcgtta
     1201 ctcatctttt ttataactat cagtatgagg taaatgagcg ggcgcgggat gtggaagttg
     1261 aaagagcgct gcgtaacgtg gtgtgtgaag gatttgatga cagcgtgatc ctgccgccgg
     1321 gggcggtgat gactggcaat catgagatgt acaaagtctt tacgcctttt aagaatgcct
     1381 ggctgaaacg gctgcgggaa gggatgccgg agtgcgtcgc tgcgccaaaa gtacgtagta
     1441 gtggagcgat agatcccgcg ccatccatta cgctgaatta tcctcgtcag tctttcgata
     1501 ctgcgcattt cccggtggaa gaaaaagcgg cgattgcgca attacgccag ttttgccaga
     1561 acggtgccgg agagtatgag caacaacgag attttccggc agtagaaggc accagtcgtt
     1621 tgtccgccag cctggcaacg ggcgggttat cgcctcgcca gtgtttgcat cgcttgttgg
     1681 cggaacagcc gcaggcgctg gacggtggag ccggtagtgt ctggcttagt gagctgatct
     1741 ggcgcgagtt ctaccgccat ttgatgacgt attatccttc gctgtgtaaa catcgtccgt
     1801 ttattgcctg gacggatcgt gtacagtggc aaagtaatcc cgcccattta aaggcctggc
     1861 aggaaggcaa aacgggatac ccgattgtcg atgctgccat gcgtcagctt aacagcactg
     1921 gctggatgca taaccggcta cggatgatta cagccagttt tctggtgaaa gatttgttga
     1981 tcgactggcg cgaaggcgag cgatatttca tgtcgcagct gattgatggt gatttggcag
     2041 ctaataacgg tggctggcag tgggccgctt ctacgggtac tgatgcagcg ccgtattttc
     2101 gtattttcaa cccgacaact cagggcgaga aatttgaccg cgaaggcgaa tttatccgcc
     2161 agtggctacc ggagctgcgc gatgtaccgg ggaaagcggt gcatgagccg tggaagtggg
     2221 cagagaaaac aggtgtgacg ttggattatc cgcaaccgat agtcgatcac aaagaggcaa
     2281 ggctgcgaac gctggcagcg tacgaagaag cgaggaaagg agcctgataa aacgcagcga
     2341 gcgttttatc aggcatggtg ctggcttatt aagactccag cgccagcgcg cggtttctga
     2401 acttaagcgc ctgatacagc caaatcatca gtaccagtcc tacacacgcc agtgcgcccc
     2461 aggtaatttg atcaaatact tcaatatatg cattgatgga gtagttgatc gccccggaag
     2521 catcaaacga agcctgcgat gtctgatcgg caatcacgcc cgccagatag ttcgcaatcg
     2581 cgccggaaag cagcatataa atgccggtta atacgccggt cacgccgggg atttcaatgc
     2641 gcgtaatctg cgacatagca accgggtcga taaacagttc cgcaaagccc atcaccgcca
     2701 ggcctaacac catcagtggc agagaagagt gaccatacat tgcggaccag cgggcgctta
     2761 aggtcagaat gcagaatccg gcgctcatca ggccaaggcc aagagcaaat tttccccaga
     2821 tgcgcacggt acgattacct gcgacgcttt ctttaaccac ccacgccagg aacacaccgc
     2881 acagcataac tgcgaaggca ttaatcgact ggaacatagc agtcggaacg gtataaccaa
     2941 acatatcgcg gttaacgaag cggtcgatat aaaggctaat cgagctaccc ccctgttgtg
     3001 cgaatgccca gaacaacatg ctgaagaagg tgagggtaac aatcagcccc agctccttac
     3061 gctgtttttg gttttctgct ttgcgataaa tttttgccag aaccccaagg ccaatgatcg
     3121 tcgcgacaat caaggcgtat accgaccact ctttccagaa caacacggta atcagcgcag
     3181 gcgttgccac cagcagaacc agtagccatc cccagttggg caggagaaag tttgtcgcac
     3241 gcagtacctt tttgttaacg ccgcgggtat gtgtgaaatg acgattgcca cataagaaaa
     3301 tgaccagacc cgcgatcata cccaccgctg ccaggccaaa gcccatcgcc caactgtact
     3361 cttcctgggc gaaaccacag gcgataggtg cgataataga ccccacgtta cccgccgcat
     3421 acatcagcga gaaaccgcca tcacgacgtg gatcggttgg ctcatacagc tcaccgagca
     3481 ggcaactgac gttggattta aacagaccat agccgcagac gataatcgcc agggacagat
     3541 agaggaatga cggatggatc tcactggcac ccagcaccac atgaccgatc gccatcaaca
     3601 acgcccccag catcaccgcc atgcgattgc cgagaacttt atccgccaaa aagccaccga
     3661 ggattggcgt gacatatacc agcgaacagt aggcgctaaa taactcgtag gcgtgattat
     3721 cgctatattt tagttgattg gtgagataga ggatcagcag ggcacgcatg ccgtaaaagc
     3781 taaaatattc ccagatttgc agcgcaacga catagtaaat agcgcgcggt tgtgatgcgt
     3841 gtttattcat aataattcca caattgactg caacgtcacg taatagttgc gcggcgtgct
     3901 tgtgtttcct taaagttaaa actttgatac agatctgatt atttatgcaa tatgctgtct
     3961 gattgcataa atatacatta gctgaagcgt gatgatataa acaatttgcg tcttttggcg
     4021 tcgggtttgt ttcttaatat gtcaaaagat tggctatcga cgaacaggcg aggtaacgct
     4081 atgttaacgg tgtagctatc agaatacgga aggcgcgaag atgaaaaaca ccgaactgga
     4141 acaactgatt aacgaaaaac tgaacagcgc ggcgattagt gattacgcgc cgaatggttt
     4201 gcaggtggaa ggcaaagaga tggtgcaaaa aattgttacc ggtgtaactg ccagccaggc
     4261 tttgctcgat gaggcagtgc gtctgggcgc ggatgcggtc attgtgcatc atggctactt
     4321 ctggaaaggt gaatctccgg tcattcgcgg aatgaagcgt aaccgtttaa aaacgttgct
     4381 ggcgaatgat atcaacctgt atggctggca tctgcccctg gacgcgcatc cggagctggg
     4441 taataacgca cagctggcgg cgttactggg gatcacggtc atgggcgaaa ttgagccgtt
     4501 ggtgccgtgg ggcgaactga ccatgcctgt gccgggactg gaactggcct cctggattga
     4561 agcgcgtctg ggacgtaagc cgttatggtg tggcgatacc ggacctgagg tggtccagcg
     4621 cgtcgcctgg tgcacggggg gtgggcaaag ttttatcgat agcgccgcgc gttttggcgt
     4681 ggatgctttt attactggcg aagtttctga acagaccatt cattcagccc gcgagcaggg
     4741 attgcatttt tatgctgcgg gtcaccatgc cactgaacgc ggtggtattc gcgcattgag
     4801 cgagtggctg aatgaaaata ccgatcttga tgtgaccttt attgatattc ctaatcctgc
     4861 ataacgaata atcagaggga tcgaaagtgc aacgagcgcg ttgttatctg ataggtgaaa
     4921 cggcggtagt actggaactg gaaccgccgg tgacgctggc tagccagaaa cggatctggc
     4981 gactggcaca gcgtctggtg gatatgccga atgtggttga agccattcct ggcatgaaca
     5041 atatcacagt gattttgcgt aatcctgagt cgctggcgct ggatgccata gagcgtttgc
     5101 aacgctggtg ggaggagagt gaggcgctgg agccggagtc tcgctttatt gaaattccgg
     5161 tagtttacgg tggcgcagcc ggaccggatt tggcggtggt cgcggcgcat tgtggattga
     5221 gcgaaaaaca ggttgttgaa ttgcactcct ccgtggaata cgtggtctgg tttttaggtt
     5281 ttcaaccggg cttcccgtat ctcgggagtt tgccggaaca actacacacg ccacggcgcg
     5341 ctgaaccgcg cttgcacgtt ccggcaggtt ctgtcgggat cggcgggccg cagaccggtg
     5401 tttatccgct ggcaacgccg ggtggctggc agttgattgg tcatacctca ctcagcctgt
     5461 ttgatccggc gtgtgacgaa cccatcttat tacgtccggg agacagcgtg cgctttgtac
     5521 cgcagaagga gggagtatgc tgaagattat tcgtgcgggc atgtacacca ccgtgcagga
     5581 tggcggccgt cacggctttc gtcagtctgg tatcagccac tgtggcgcac tggatatgcc
     5641 tgcgttacgc attgctaacc tgctggtggg taatgacgcc aatgcccccg cgctggagat
     5701 cacgctcggt cagttaacgg ttgagttcga aactgatggg tggtttgctc tgacgggtgc
     5761 cggttgcgaa gcgcggctgg atgataatgc cgtctggacc ggctggcgat tgccgatgaa
     5821 agcaggccag cgtttaacgc ttaaacgccc gcagcacggg atgcgcagtt atctggcggt
     5881 cgcgggtggt attgatgttc cgccggtaat gggatcatgc agcaccgatc tcaaagtggg
     5941 gattggcggg ctggaaggcc gtttactgag ggatggtgac cgactcccga ttggcaaagc
     6001 gaagcgtgat tttatggaag cgcagggcgt taaacagctg ctctggggca accgcattcg
     6061 cgccttgccg gggccggaat atcatgagtt cgatcgcgcc tcgcaggatg cattctggcg
     6121 ttcgccctgg cagcttagcc cgcaaagtaa ccgcatgggc tatcgcttac aggggcaaat
     6181 tttaaaacgc accaccgatc gcgaactgtt atctcacggt ttgttgccgg gcgtggtgca
     6241 ggtgccgcat aacggccagc cgattgtatt gatgaacgac gcacagacca ccggtggtta
     6301 cccgcgtatt gcctgtatca ttgaggctga tatgtaccat ctggcgcaaa ttccgctcgg
     6361 tcagccgatt cattttgtcc agtgttcact ggaagaggca ctgaaagcgc ggcaagatca
     6421 gcaacgttat ttcgaacaat tagcgtggcg gctgcacaat gaaaattgac ctgaacgccg
     6481 atctgggcga aggctgcgcc agcgacgcag agctattaac gctggtttcc tctgccaata
     6541 ttgcctgtgg atttcatgca ggagatgcgc aaaccatgca ggcttgcgtg cgtgaagcaa
     6601 taaaaaatgg tgtcgcgatt ggcgctcacc cgagttttcc cgacagggaa aattttggtc
     6661 gcagcgccat gcagctgccg ccagaaacca ttttcgccca gacgctgtat caaattggcg
     6721 cgctggcagc gattacccgt gcgcaaggcg gcgtaatgtg tcatgtcaaa ccgcacggca
     6781 tgttatacaa ccaggcggcg aaagaggcac aactggcaga cgctatcgcc agagcggtat
     6841 acgcttgcga tccggcattg attctcgtcg ggctggcagg aagcgagctg attcgtgcag
     6901 gcgaacgata tggcctggta acgcgcgagg aagtgtttgc cgatcgtggt tatcaggcag
     6961 acggttcgct ggtgccgcga agccagccag gcgcgttgat tgaaaacgaa gaacaggcgc
     7021 tggcgcaaac gttggagatg gtgcaacacg gcagagtcaa aagtatcacc ggcgaatggg
     7081 caatggtcac ggcgcaaacg gtctgcctac atggcgatgg cgagcacgca ctggcgttcg
     7141 cccgccgatt acgagctaca tttgctgaaa aggggattgt tgtcgcagca taaccccgct
     7201 taataaagaa tgaaaaaagg atatcaccat gcctgaaggc ccggagatcc gccgtgcagc
     7261 ggataacctg gaggcggcga tcaaaggcaa accactaact gatgtctggt ttgccttccc
     7321 gcagttaaaa tcttatcaat cacgacttat cggtcaacac gttacccatg tggaaacgcg
     7381 tggtaaggcg ttgttaactc atttttccaa cgacttaacg ctctacagcc ataatcagct
     7441 ttacggcgtc tggcgcgtgg ttgataccgg cgaagagccg cagaccacgc gagtattgcg
     7501 ggtaaaactg caaacggctg acaaaaccat tctgctttat agcgcctcgg atattgagat
     7561 gttgactccg gaacaactga ccacgcatcc gtttttacaa cgcgttggtc ccgatgtgct
     7621 ggatccgaat ctgacgccgg aggtggtaaa ggaacgatta ttgtcgccgc gctttcgtaa
     7681 ccgtcagttt gctggattac tgctcgatca ggcgtttctg gcaggacttg gcaattattt
     7741 gcgggtggag atcctctggc aggttgggtt gaccggaaat cataaagcga aagatctcaa
     7801 tgcgccgcaa ctggatgcac tcgcacacgc gttactggat attccgcgac tttcttacgc
     7861 tacgcggggg caggtggatg aaaacaaata tcatggggcg ctgtttcgct ttaaggtttt
     7921 tcatcgtgat ggcgaacctt gcgaacgctg tggcggcatc attgagaaaa ccacgctgtc
     7981 atcgcgcccg ttttactggt gcccaggctg ccagcactag gccgaacgct tcggcgcata
     8041 ggttgaaata aaccgtgcga tggcaggccc cgtcagcaaa atactgaaca ggcgcagggt
     8101 ttgcatcgca atgatgagcg ccatatcggc attgctccct gcggcgatga ccgccacggt
     8161 atcaagcccg ccggggctgg tggcgaggta ggcggtcata aaatcaatat gcataaaccg
     8221 ggtcagcccc cacgccatgc ccgcacaaat agccagcaga gcaaaaatcg acagcaggat
     8281 ttgcggtagc gggcgcaatg cccgcagtaa tatttgctta tcgaaaccaa gaccaatccg
     8341 ccagccaatt gccatatatg ccatcgccag cagccattcc gggagttcaa tcgtgatgag
     8401 ctgaccagac tggagcactg cgcccgccag catcgggatg agcatcgtgc cggaaggaag
     8461 acgtagcaag catcccgccg taccggcaac gaccgcgagc aaaatcgtaa gcaggagatt
     8521 aatgctcact ggcgggaacc agacgatctg ctggttaacc gcttcagcgt tatcgcccag
     8581 catcatgcgg gtgaccagaa cagcggctcc tgcgacgaac agcactcgca gatattgcat
     8641 aaacgccacc aggcgaatat ctgcgccgta atcttgcgcc attgcgacca ttgccgccgc
     8701 gccgccagga gaagaacccc aggccccggt attcccaggc agtgagctat agcgcaccaa
     8761 taaccaaccc acgatggcgc tggaaagcag cgtcaccagt aaaatcgcaa gcacgatcgg
     8821 ccagttgacg gccagcgtgg tgagaatcga accagtgagg ttttgtgcaa tcatgcagcc
     8881 aagaatggct tgggcagcga gaaaagcgga gcggggaagt tgcagagtga ctccgtgcat
     8941 actgaagatg atccccgcga tcatcgggcc aagcaggagt gccgccggga gatgtaaagc
     9001 gaggaagcca atagaaagga gaagtgacag aacacataac attccccact gtagcactgg
     9061 catccgtcac tccctgttta acttaccttt ggtgtatcga tacagcataa agaatgcgcc
     9121 tcaacgaaaa aatagcggta atcaagaaaa agacagattt tggctaaagg gcgtattaag
     9181 taccgttgtc tccctctcgt ttgtaaaaga gggaagagta aacgattgaa ctgagataag
     9241 tcttatgacg aggaattatt gatcagtgca ctgaccgggc tgtcgaagtg tgatttcatg
     9301 gcttgagtcg ccgtttcgac atccctggca cacacagcat cgcagatttt ctggtgtagt
     9361 gtgatgatgt gattgccata ctctttctga tcgctttggg cgtttaaacc ctcctggatg
     9421 gattgccgca actgctgatg taatgcctgc aataacatca taaaaagcgg attttttgag
     9481 gcaacggcaa gtaactgatg gaagcggata tcatgggtga taaagctttc aacatcattg
     9541 aactgttcgc ggatttttgc catttcagcc cgtaggttag caaaatcttc ctcttcgcca
     9601 ttacgggcag cagcttccac aacgcccaac tccagccagc ggcgtacttg aagaatttgc
     9661 tctgttgtca cttgttcggt cgccagggca aatccaaaca ccacgcgcag cacttcatca
     9721 tcaaattttt tgattcgtgg cactttaccg ctgtgcgttt ccagaatccc cagggcggca
     9781 agtgaacgat aggcctcacg cacaataccc cgactgacac ccaatttctg aatcagctga
     9841 acttcactgg gaactggcat ccccggtgtc aatttttgtt cggcgatcag attaagaata
     9901 tgatttttga cctgtgacga taacgttacg ctggtcaata acgcactctc actcattttt
     9961 actcctgttg tactttttcc tggtcggtga aatccagaat accgggactg tatccttcat
    10021 attttcctcc agaacccttg attatcaata gactactgcg cctgtgcgcc cgccatgcag
    10081 tgcgaatcaa agacatattg tgtgaaagag tggcgaagaa atagtaaata cacaactttt
    10141 tcgtgacgcc cttcgcagta ttgtgcgctt tttctcctta ccctgtaaac ctgttaaaca
    10201 ggcttacagg acgaaaataa tgtggaagag tgataacgaa ctttttgctt tgatgaaaac
    10261 ccggctattt accgccgtgg ttggcgatat tctcgacacg ctgggctata tgcaccagtt
    10321 tgtttctccg gcaatcaagc cgctggatgt gaatacggtg gtggcaggcc gggcaatgcc
    10381 ggtgctggag tcggacgttt acgaaaccaa atcgacgtcg ggtaagacgg cgattggcga
    10441 aaaaccgttt ggcatcatgt ttgaagcgct ggatgccctc aaagagaacg atgtttacat
    10501 cactacgggc agctctctgc gctatgcctt gtggggcggg ttgatgtcta cccgtgccat
    10561 gcatctgaaa gcagcaggtg cggtactcga tggttacgcc cgtgactctg ctgaaattct
    10621 tgaattgaaa tttcccactt tttgtcatgg cacttacgca caggatcaag gtccgcgcgg
    10681 caaggtcatc gacttcggcg tacctattga atttcacggc gtgcgagtga atccgggcga
    10741 tatcgtgttt ggtgatcgtg acggattact gattattccc caggttgttg agaaagaagt
    10801 tattacccag gccctggaaa aagtggcaac tgaaagtgaa gtgcgcaaag ccatcagtga
    10861 tggcatgagc acggtacagg cctttgaaac cttcggcgta atgtaaggag agcaatgaat
    10921 gaaggcatta accattgagc agggaagtca ggcgcgattt gttgatatcc ccgaacctgt
    10981 ctgcggtaaa ggagaagtgc taatcaatgt tgagcgtgtc ggactgtgtg gttctgacct
    11041 caatacctgg cggggactaa atccgctggt taattatccc tgtattccgg ggcatgaaat
    11101 tggcggcacg gtgctggcaa ctggcgatat ggcaggggcg atcaagccag gtgaccgggt
    11161 ggtcgttctg ccttgcactg agtgtggcgt gtgcagctcc tgtctgagcg gtcgtcctaa
    11221 cgcctgtaaa aacaaccaaa ctcttggtgt gcaacgtcag ggggggatgg cgcaacaact
    11281 gacgatgcct gcacataaag tcattgtctg taatgcctta agtgttgatg agttagcgct
    11341 ggtagaaccg ctggccgtgg gactacatgc ggccagacgt gcgcaaatta atgtgggtga
    11401 atgggtcgca gttatcggtt gtggggtaat tggccttggc gctatcgccg cggcatccgc
    11461 catgggggcg cgcgtaattg ctatcgatat tgatgatcgc aagcggaaca ttgccctgga
    11521 atgcggtgcg caacgttttc ttaattcgca gcaatgtgac gtggcggcag aactggtgct
    11581 gttaaatgac ggtcgcggcc cggaagtggt gattgaagcg gtgggtaatc aacaaaccat
    11641 tgaacaggct acagggttgg tggcgtttag cggtcgtgtg gtttatgtcg gttacgccaa
    11701 acagccagtg agttatcaga gtgcgcagtt cctgatgaaa gaactggata tccgtggttc
    11761 gcgcaatgcc accgtggcgg attttcgtgc cgtgctggca ttgatggagt ccgggcgtta
    11821 tccggtcagc aatctggtga gtgctcacta cccgctggaa caggctgatc acgcttttag
    11881 cgactgggca agtgatcccg gcgcgtatac caaaatcctc attgaccttt aaggagcgac
    11941 accatgaatg ctttttcttt acataacaaa accgcgttga tcaccggtgc agccagtggg
    12001 ctggggctgg caatggcgaa atgcatgata gtcagcgggg cgaaggtcat tattgctgac
    12061 ctgaacggcg agctggcgca agcgaccgcc ggaacattgg ggtcgcaggc cagttggata
    12121 caaatggatg tcggggatac cgcagccgcg caggaaaaag ttgatgtgct tattagcgaa
    12181 cacggggcta tcgatatcct ggtcaataac gcaggcaatc actgcaaaaa accgattgaa
    12241 gagatgagcg tcagtgattt tgagtcagta ctcgacgttc acgtagtcgg cgcatttgcg
    12301 ttaaccaaag cgctggtgcc gcatatgaaa caacgcggta aaggctgtgt gctttttact
    12361 gcatcgatga cctcatttct tgggcagcct ctggttactg gctacagcgc agcgaagtca
    12421 gcctatctcg gcatgattcg cggtatgacc acggagcttg ccgttcatgg gattcgcgtc
    12481 aatggtattg cgccaggctg gatcgataca ccaatgttgc gtaaggccat tgaaggtgat
    12541 gacgaacgcc gtaacaaaat cctgggtcgt accccgatga aaaaattcgg caagccggag
    12601 gatatcggtt gggctgcggt ttatctggct tcagacgcag cggcgtttgt cagtggtcag
    12661 gtgctggtgg tggatggcgg cgcattgatc ggcttctgat gtacggagga attatgcgca
    12721 aacgtgcgtt aaagctacat gccaaagata actgtgtagt cgcgctgaat gacatcgcca
    12781 gtggcgatat tgtctggtgg gatgaggggg aaatggtggc ctgtagttcc gttaccctgg
    12841 ggcacaaact ggcctgcgaa gcgctgaaca atggtgattc gattctgaaa tacggcgcca
    12901 ttatcggaca cgccactcgc gatattgcca tgggtgaaca tgtgcattcc cataatcttg
    12961 ccagcaatgc catcgccatt tttcatcatg atgatgccgg ctatcagtca gaggaaaaag
    13021 cataatggaa ggttatgtac ggcaaaatgg tcgaccaggt attcgtaata ctgtgcaggt
    13081 aatctttatg gtggagtgtg ccagccatgt cgctaaaaag attgccgaag gatttccttg
    13141 tcacgacgtg cagtattttg gtttttcagg ttgttatccc agcgattatg gctatcggct
    13201 ggtgaaaaat ctggcgactc atagcaacgt aggtggtgta ctgattattt cgctggggtg
    13261 cgagaatttc gatcgccgtc ggctggagca agaagtacga gaaagtgggc gaccgtgcca
    13321 tacgctggtc attcaggaaa ataaaggcac gaccaacacc attactctgg gtaagcagtt
    13381 ggtcgctgaa atgctggaac agcttgcgga tacaccgcgt tgtatcctga actggagcga
    13441 cctggtggtt ggtactatct gcggtggctc cgatggcacc agtggtatca cgggtaaccc
    13501 ggcagtagga cgtgcctttg accaactgct ggagaaaggg gctacctgta tttttgaaga
    13561 aagcggtgag ttgcttggtt gtgagcagca tatgatgtcg cgcgcggcta gttcgcaggc
    13621 acgtgatgcc atcgaagtgg caatgacaaa agccgaacgt tattacagtt tgatggggct
    13681 ggggagtttt tctaacggta atgctgaagg cggcttaacc actcaggaag aaaaatccct
    13741 tggggcttat accaagtcgg gcgatgcgct gattgctggc gttattttgc cgacgcaaca
    13801 aacgcccttt cctgggcttt ggttgatgga cgtggtgcct gatggtgaac ctcgttttgg
    13861 gtatccgaat attaacgatt cctcagaagc actggaactt atcgcctctg gctgtcatat
    13921 ggtgttattt accacaggtc gcggttcagt tattggttca gtgatcgctc ctattattaa
    13981 agtgtgtaat aacccgcaga cgtgggagca tttatctgac gatatggacg ttaatgccgg
    14041 tcgtgtaatt agcgaaggcg caacgctcga cgatattgca gacgatatta tgcaggtgat
    14101 tgaacatgtc gccaacggtc aatattgtgc cgcagaacga ctggaacatc aggagtttgt
    14161 tttaacgtat aaagcatttg attacagcaa aagtgcagtc tgtcgggcag atccccgtta
    14221 atcgataaag ctcttacagg taataataat gaaaaaatta ctgacaccac ttttatttct
    14281 tttagcttta tttagtccga tgctgaatgc cgcaccggta actatcaaaa tcgcctatga
    14341 aagtaacccc ggcgagccag cggatacagt agtgaagcac tgggcagaac ttgtgaagaa
    14401 aaaaagcaat ggagatattg ttttcgaact ttacccaagt tcacagttag gttctaaaca
    14461 agatattact gaacaagcgt tgatggggat gaatattatc accatcagtg atgtaggatt
    14521 tttaactgac tacgatgcag acttgggaat attgtttggg ccttatttaa ccgaagatcc
    14581 gcagaaactg tttgcgattt atgccagtga ctggtttaaa caaaaagaac agaaactacg
    14641 ggaaaaaggt gtgcatattg tcatgagcaa ctatctgtac ggtaccaggc agatcattgc
    14701 taaaaaaccg attcgtacac ctgaagactt gaaagggatg aagatccgcg tgccgaataa
    14761 cgtcatgcag attaaagcta tcgaagcgat gggcgcaacc ccaacaccaa tgccgctggg
    14821 agatgtttat ccggcgctga ctcaggggat gatcgatggt gtggaaaacc cggtttctgt
    14881 tctctatggg caaaagctgc atgagcaggc gaaatatctg tcaatggtga actatctgac
    14941 caatacatca ttatggttgg gtggcgaagc gtttttcagt accctgacac ctgcgcagtt
    15001 agatattatt caccagacag cgtatgaagc tggtttatat agccagaaaa ttaccactga
    15061 gcaggatgcg acaatgttga aaaccatgca ggagcagggc gtggaaatca tctatcctga
    15121 cgtcgcccct ttcaaagaaa aagccttaaa agtctatcaa caattcccgg aatggacccc
    15181 tggtttgtat gacactattc agcaacagct gaaataactt catggcataa ggactggtcg
    15241 gtgtttcggc accgaccgtt aagagcagag gtgtttatgc gtcttttagg gaaaatgatt
    15301 gaagttgctg cggtattaac gctctttata ttggtaatca tgaccattgc tgcggtaata
    15361 atgcgttact gttttcatat gccattacag tggacagaag aggtttccgg tttattaatg
    15421 atttgggtag tcatgtttgg tgcggttgtt gctgaacgag ataataccca tttatcgatt
    15481 ccatttttag ccgatgcact tacttcacgc gggcaacgta tattagcgtt tttagtctct
    15541 ctgatgtcta ttattttgct ggtggtcatg gcctggtcag catggaaatt agctgccggg
    15601 acagccttta aaaccacgca aatattaaaa atatcctggt tttggatcga tattgccgtc
    15661 acggttggcg ctatcggtgt cgcctgtttt actgccttac aactgatcaa gaaaacgcca
    15721 cgcaaagaag gtgaataatg acagcattga gtgaattact ggcgggaagc tggctggata
    15781 actggctgat gatcattccg cttatgttcg tctgtttatt tatcaatatg ccggtatggc
    15841 tggcgatgtt ttgcggcatt ctgacctact tcctttgcta ttcaaatgtg ccgttaatgg
    15901 tggcaattca gcgtttgatt gccacgacgc aaaccccttc gctgttagct attccgtttt
    15961 ttatcttatt aggcacgtta atgagctata ccggtattgc cgcgcgtatt ttgcaaattg
    16021 ccaacgtgtt gattggcaga atgcgcggtg gcctgggtgg ggctaatatt ctggtcagta
    16081 ccatgatggg cggtgtcagt gcctcaaatc tggcggatgc cgcaatgtta tcgcgcatga
    16141 tggtgccgga aatggaacag aaaggttatt cccgtggctt tgcggcggcg ataacggcaa
    16201 gtggttcgct gattacacca attattccac cgggtattgc actgatcctt tacggactgg
    16261 tggcggatgt ctccatcggc tccatgttta tggccgggat cctgccaggt ctgctgtgct
    16321 ccgccatgtt gatcgttacc gtatggttgg tggctattcg tcgcggttta aaaccttcca
    16381 gcgaacactg gccaaaaccg aaagaagtgt cagtggcgct ggtgcaggcc tggccagcac
    16441 tgttcttaat tgttgcggtt atcggtggta ttcgggcaaa tatctttacg ccaacggaag
    16501 ccggagcggt agcagtatta ttggtaatgt ttattggttt tgtaatttat cgcgaactga
    16561 aaatcagcca tgtatttaca gcactctctg agacggcgcg ttccacggca tcggtcatgt
    16621 taatcattat ggcgagtgcc gcgctgggct ggatattttc ccttgagcgg gcaggtatca
    16681 cggttgcgga gtttgtaact tctctgaccg ataataaata cgtcttttta ttgattatca
    16741 atctattgct gctggtgctt ggcatgttcc ttgaaggtag tgccattctg ctgatattag
    16801 tcccattact taaacccgtg gtggctcatt ttggcattga tccggtgcat tttggcatca
    16861 ttatgatcct caacttatcg ttaggaacgt tatcaccacc tgtcggaacg gtgatgctgc
    16921 tggtatgtaa taccgtacag gtttctgtcg ggcaatttat gcgtgaagcg gtgccattac
    16981 tgatcagtct tattttactt ctgttggtca ttacctatat tccggcaata tctttgctgc
    17041 tggtgtagaa ttaaaaaccc tgcgtgtcca cgcagggttt tgtcggtgtc atttttactt
    17101 cttaatatcc gacttaaaat cacgctgcgc gtagccggta tacagctgac gcggacgggc
    17161 aatcttcata ccgtcactgt gcatttcgct ccagtgggcg atccagccaa ccgtacgtgc
    17221 catagcgaag atcacggtga acatagaaga cggaataccc atcgctttca ggatgatacc
    17281 agagtagaaa tcgacgttcg ggtacagttt tttctcgata aagtacgggt cgttcagcgc
    17341 gatgttttcc agctccatag ccacttccag caggtcatct ttggtgccca gctctttcag
    17401 cacttcatgg caggtttcac gcattacggt ggcgcgcggg tcgtagtttt tgtacacgcg
    17461 atgaccgaag cccatcaggc ggaaagaatc atttttgtct ttcgcacgac gaacaaattc
    17521 cggaatgtgt ttaacggagc tgatttcttc cagcattttc agcgccgctt cgttagcacc
    17581 accgtgcgca ggtccccaca gtgaagcaat acctgctgcg atacaggcaa acgggttcgc
    17641 acccgaagag ccagcggtac gcacggtgga ggtggaggcg ttctgttcat ggtcagcgtg
    17701 caggatcaga atacggtcca tagcacgttc cagaatcgga ttaacttcat acggttcgca
    17761 cggtgtggag aacatcatat tcaggaagtt accggcgtag gagagatcgt tgcgcgggta
    17821 aacaaatggc tgaccaatgg aatacttgta acacatcgcg gccatggtcg gcatttttga
    17881 cagcaggcgg aacgcggcaa tttcacgatg acgaggattg ttaacatcca gcgagtcgtg
    17941 atagaacgct gccagcgcgc cggtaatacc acacatgact gccattggat gcgagtcgcg
    18001 acggaaagca tggaacagac gggtaatctg ctcgtggatc atggtatgac gggtcaccgt
    18061 agttttaaat tcgtcatact gttcctgagt cggtttttca ccattcagca ggatgtaaca
    18121 aacttccagg tagttagaat cggtcgccag ctgatcgatc gggaaaccgc ggtgcagcaa
    18181 aataccttca tcaccatcaa taaaagtaat tttagattcg caggatgcgg ttgaagtgaa
    18241 gcctgggtca aaggtgaaca caccttttga accgagagta cggatatcaa taacatcttg
    18301 acccagcgtg cctttcagca catccagttc aacagctgtg tccccgttga gggtgagttt
    18361 tgcttttgta tcagccattt aaggtctcct tagcgcctta ttgcgtaaga ctgccggaac
    18421 ttaaatttgc cttcgcacat caacctggct ttacccgttt gttatttggc tcgccgctct
    18481 gtgaaagagg ggaaaacctg ggtacagagc tctgggcgct tgcaggtaaa ggatctattg
    18541 atgacgaata aatggcgaat caagtactta gcaatccgaa ttattaaact tgtctaccac
    18601 taataactgt cccgaatgaa ttggtcaata cttccacact gttacataag ttaatcttag
    18661 gtgaaatacc gacttcataa cttttacgca ttatatgctt ttcctagtaa tgtttgtaac
    18721 aactttgttg aatgattgtc aaattagatg atta
//
LOCUS       DACADA010000081        18471 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.082, whole genome shotgun sequence.
ACCESSION   DACADA010000081 DACADA010000000
VERSION     DACADA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.082"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            55..1194
                     /gene="arnB"
                     /locus_tag="ISB17_002836"
     CDS             55..1194
                     /gene="arnB"
                     /locus_tag="ISB17_002836"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAP1980742.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1198..2166
                     /gene="arnC"
                     /locus_tag="ISB17_002837"
     CDS             1198..2166
                     /gene="arnC"
                     /locus_tag="ISB17_002837"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAP1980743.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2166..4148
                     /gene="arnA"
                     /locus_tag="ISB17_002838"
     CDS             2166..4148
                     /gene="arnA"
                     /locus_tag="ISB17_002838"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAP1980744.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4145..5035
                     /locus_tag="ISB17_002839"
     CDS             4145..5035
                     /locus_tag="ISB17_002839"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAP1980745.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5035..6687
                     /gene="arnT"
                     /locus_tag="ISB17_002840"
     CDS             5035..6687
                     /gene="arnT"
                     /locus_tag="ISB17_002840"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAP1980746.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            6684..7019
                     /gene="arnE"
                     /locus_tag="ISB17_002841"
     CDS             6684..7019
                     /gene="arnE"
                     /locus_tag="ISB17_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAP1980747.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7019..7405
                     /gene="arnF"
                     /locus_tag="ISB17_002842"
     CDS             7019..7405
                     /gene="arnF"
                     /locus_tag="ISB17_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAP1980748.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7399..7665)
                     /gene="pmrD"
                     /locus_tag="ISB17_002843"
     CDS             complement(7399..7665)
                     /gene="pmrD"
                     /locus_tag="ISB17_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAP1980749.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7775..9130)
                     /gene="menE"
                     /locus_tag="ISB17_002844"
     CDS             complement(7775..9130)
                     /gene="menE"
                     /locus_tag="ISB17_002844"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAP1980750.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9127..10089)
                     /gene="menC"
                     /locus_tag="ISB17_002845"
     CDS             complement(9127..10089)
                     /gene="menC"
                     /locus_tag="ISB17_002845"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAP1980751.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10089..10946)
                     /gene="menB"
                     /locus_tag="ISB17_002846"
     CDS             complement(10089..10946)
                     /gene="menB"
                     /locus_tag="ISB17_002846"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAP1980752.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10961..11719)
                     /gene="menH"
                     /locus_tag="ISB17_002847"
     CDS             complement(10961..11719)
                     /gene="menH"
                     /locus_tag="ISB17_002847"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAP1980753.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11716..13386)
                     /gene="menD"
                     /locus_tag="ISB17_002848"
     CDS             complement(11716..13386)
                     /gene="menD"
                     /locus_tag="ISB17_002848"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAP1980754.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERGRFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13475..14770)
                     /gene="menF"
                     /locus_tag="ISB17_002849"
     CDS             complement(13475..14770)
                     /gene="menF"
                     /locus_tag="ISB17_002849"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAP1980755.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14849..15154)
                     /gene="elaB"
                     /locus_tag="ISB17_002850"
     CDS             complement(14849..15154)
                     /gene="elaB"
                     /locus_tag="ISB17_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAP1980756.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15209..15670)
                     /locus_tag="ISB17_002851"
     CDS             complement(15209..15670)
                     /locus_tag="ISB17_002851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1980757.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15735..16652
                     /gene="rbn"
                     /locus_tag="ISB17_002852"
     CDS             15735..16652
                     /gene="rbn"
                     /locus_tag="ISB17_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAP1980758.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16773..>18471)
                     /locus_tag="ISB17_002853"
     CDS             complement(16773..>18471)
                     /locus_tag="ISB17_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1980759.1"
                     /translation="LSGCGPEPEDKKSQQQQSTTPTDQQVLAAQQAATKVAEQSAAKA
                     AADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQIANPATARYQQFDDNP
                     VKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYFPSDWDIKDKQ
                     SIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLVFLIDTSGSMI
                     SDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDA
                     EGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESG
                     VTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVK
                     AQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKHITLLFELTLKGQKAS
                     IDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPLASAIKAPSEDMRFRA
                     AVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFIRLIGLAKDLDNSQN"
BASE COUNT         4250 a         4978 c         4684 g         4559 t
ORIGIN      
        1 cactctttat agcaaatata agtggacagg tattcaatga cggatggaaa aacaatgtca
       61 ggatttttgc ctttttcgcg accagcaatg ggcgtagagg aactcgctgc agttaaagag
      121 gttctcgaat ccggttggat cacaaccggt ccgaagaatc aggcgcttga acaagctttt
      181 tgccagttga cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat
      241 atcacgctaa tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc
      301 tgggtttcaa ccctcaatat gatttccttg ctgggtgcaa cgccggtaat ggtggatgtc
      361 gaccgcgata cgctgatggt cacgcctgaa gcgatcgaag cagccattac accacgcact
      421 aaagccatca ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc
      481 attggcgaac gttacggcat cgcagttatc gaagatgctg cccatgccgt aggtacgtat
      541 tacaaagggc gacatattgg cgcaaaaggt accgctattt tttcgtttca cgccatcaaa
      601 aatattacct gtgcggaagg tggcctgatt gttactgata atgaaaatct tgcccgccag
      661 ctgcggatgc tgaaatttca cggtctgggt gtcgatgcct atgacagaca tacctggggc
      721 cgtgcaccgc aggctgaagt cttaacgccg ggctataagt acaatctgac cgatattaac
      781 gccgcgattg ccctgacaca gttagtcaaa ttagagcacc tcaacacccg gcggcgcgaa
      841 attgcccagc aatatcagca agcactggca actctcccct ttcagccatt aagccttccc
      901 gcctggccgc acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt
      961 ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg taccgggtta
     1021 catttccgcg ctgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta
     1081 ccgaataccg aatggaatag cgaacgcatc tgttcattgc cgctgttccc ggatatgacc
     1141 accgccgatg ccgaccgcgt catcacagcc cttcagcaac tcgcaggaca ataagccatg
     1201 tttgaaatcc accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa
     1261 agcttaccgg aattaatcag gcgcaccacc gcagcctgtg aatcactggg gaaagagtat
     1321 gaaatcctgc tgattgatga cggcagtagc gataattccg cgcatgtgct ggtcgaagcc
     1381 tcacaagcgg agggcagcca tattgtgtct attttgctta accgcaatta cgggcaacat
     1441 tcagcgatta tggcgggatt cagtcacgtc accggcgact taattattac cctcgatgcc
     1501 gatctccaga atccgccaga ggagatcccc cgtctggtgg caaaagccga tgaaggttac
     1561 gacgtggtag ggactgtacg ccagaaccgc caggacagct ggtttcgtaa aaccgcttcg
     1621 aagatgatta accggcttat tcagcgcacc accggcaaag cgatgggtga ctatggttgt
     1681 atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgcagc
     1741 acctttatcc cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtgcac
     1801 catgccgagc gtgagtttgg tgaatccaaa tacagcttta tgcgcctgat taatttgatg
     1861 tacgacctgg tgacctgcct taccacaacg ccgctacgta tgttgagttt gctcggcagc
     1921 attattgcga ttggcggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc
     1981 ggaccacaat gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt tacttttatt
     2041 ggcgctcagt ttatcggcat gggattgctc ggtgaatata tcggcaggat ctacaccgat
     2101 gtccgcgccc gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat
     2161 gaataatgaa aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagccc
     2221 tgctgtctgc cggttacgaa attagcgcca tttttaccca tactgataac cccggtgaaa
     2281 aagcctttta tggttcggtt gcccgactgg cagcggaaag aggcattcca gtttatgcgc
     2341 cggataacgt taatcatccg ctgtgggtgg aacgcattgc ccaactatca ccagaggtga
     2401 ttttctcttt ttattatcgc catcttattt gcgatgagat tttgcagctc gctccgagag
     2461 gtgcatttaa tctgcatggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact
     2521 gggtgctggt gaacggtgaa acggaaactg gcgttacatt gcaccgaatg gtgaaacgtg
     2581 ccgatgccgg ggccattgtg gcacaactgc gcgttgccat tgcgccagac gatatcgcca
     2641 ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag acattacccg
     2701 ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc acctgttttg
     2761 gtcgcagaac gccggatgac agtttcctcg aatggcataa gccggcatcc gtactgcaca
     2821 acatggtgcg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga
     2881 aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcactg ccggggagcg
     2941 tgatttctgt tgcaccactg ctgattgcct gtggcgatgg cgcgctggaa atcgtcactg
     3001 gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg
     3061 tgcaaggttc acgcttgaat agccagcctg cctgtgccgc ccgacgccgt acccgggtac
     3121 tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag
     3181 atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc
     3241 atccgcattt tcactttgtc gaaggcgata tcagtattca ttccgaatgg attgagtatc
     3301 acgtcaaaaa atgtgatgtc gtgttgccgc tggtggcgat agccacgccg attgaatata
     3361 cccgcaaccc gctgcgcgta tttgaactcg atttcgaaga gaatctgcgc attatccgct
     3421 actgcgtgaa gtaccgtaag cgaatcatct tcccgtcgac ttcagaagtt tatgggatgt
     3481 gtagcgataa atacttcgat gaggatcatt ctaatttaat cgtcggcccg gtgaataaac
     3541 cacgctggat ttattcggtg tctaaacaat tacttgatcg ggtgatctgg gcctatggcg
     3601 aaaaagaagg tttacagttc accctcttcc gcccgtttaa ctggatgggg ccacgactgg
     3661 ataaccttaa tgcagcgcga attggcagct cccgcgctat tacgcaactc attctcaatc
     3721 tggtagaagg ttcaccgatt aagctgattg atggcggaaa acaaaaacgc tgctttactg
     3781 atattcggga tggtatcgag gcgttatacc gcattatcga aaatgcggga aatcgctgcg
     3841 atggcgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgaa gaactgggtg
     3901 agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca ttacttccca ccgtttgcgg
     3961 gctttcgtgt tgtcgaaagt agcagctact atggcaaagg atatcaggac gtagagcatc
     4021 gtaaaccgag catccgcaat gcccgccgct gcctggactg ggaaccgaaa attgatatgc
     4081 aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac
     4141 catcatgacc aaagtcggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg
     4201 cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag
     4261 cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg
     4321 gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac tggcaggtac
     4381 ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa
     4441 acatcacgaa gtcggcctgc acgcctggga tcaccatgcc tggcaagccc atagcggtaa
     4501 ttgggatcgg caaacaatga tcgacgatat tgcacgcggc cttcgcaccc tggaagagat
     4561 tatcggtcaa ccggtaacct gttctgccgc tgctggctgg cgtgctgacc aacaggtgat
     4621 cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg cgatgccgtt
     4681 tcgtccattg ctcgaatcag gaacccccgg cactgcgcaa attccggtga ccttacccac
     4741 ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa
     4801 ccgcatccag cgagataaag gcacgccggt ttataccatt catgccgaag ttgaaggctg
     4861 cgcttatcag cataattttg tggatctcct gaaacgcgca gctcaggaag gcgtgacatt
     4921 ttgcccttta agcgaactgt tatcagggac gttgccgctc ggacaagttg ttcgcggaaa
     4981 tattgccgga cgtgaaggct ggctgggttg ccaacaaatg gcgggtagtc actgatgaaa
     5041 tcggtacgtt accttatcgg cctcttcgca tttattgcct gctattacct gttaccgatc
     5101 agcacgcgtc tgctctggca accagatgaa acgcgttatg cggaaatcag tcgggaaatg
     5161 ctggcatccg gcgactggat tgttccccat ctgttagggc tacgttattt cgaaaaaccc
     5221 attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt
     5281 gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctgatgac ctggtttact
     5341 ctgcgcttat ggcgcgataa acgtctggct ttactcgcca cagtaattta tctctcattg
     5401 tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc cttctggctg
     5461 gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc
     5521 gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc
     5581 gcccttgccg tgccggtatt aagcgtgctg ccatgggtag caacgcaaaa acgctggaaa
     5641 gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg
     5701 ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat
     5761 attcaacgct ttgcactgga tgatgcccaa catagagcgc cgttctggta ctacttgccg
     5821 gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacgctggc
     5881 tggaaaaacc gcaagcattc cgcaaccgtt tatttgttga gctggacgat aatgccgctg
     5941 ctgtttttct ccgtcgctaa aggcaaattg cccacctata ttctttcctg ctttgcacct
     6001 ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgttg
     6061 cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg
     6121 gtctccccct ggggaccaat gaacacaccg gtgtggcaaa ccttcgagag ctataaagtc
     6181 ttttgtgcct ggtctatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac
     6241 gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga
     6301 ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca
     6361 caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt
     6421 ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa
     6481 tatggcctta attatccgga tgcaaaaggg agatttgtca gcggtgatga gttcgcaaac
     6541 tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat
     6601 gaagatatca atagtctcgc cattccgccc gctgatgcca tcgatcgtca gggacgtctg
     6661 gtactcattc agtatcggcc caaatgatct ggctaacatt agtctttgcc agcttgctta
     6721 gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgcggcggta aacaaacggc
     6781 gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggcctt gccatggtgc
     6841 tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga
     6901 attttgtctg ggtaacgctg gctgcagtaa aactgtggca cgagccggta tcgctgcgtc
     6961 actggtgtgg ggtggcgttc attattggcg gcattgtgat cctcgggagt acggtgtaat
     7021 gggcctgatg tggggattat tcagcgtcat tatcgcctcc gctgcgcaat taagcttggg
     7081 ttttgcggcg agtcatctgc cgccaatgac gcacttatgg gattttatcg cggcgctgtt
     7141 agcttttggc ctggatgccc ggatattgtt actcgggctg ctgggttatc tgctttccgt
     7201 tttttgttgg tataaaacac tacataaact tgcgctaagc aaagcctatg ccttgctcag
     7261 tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt
     7321 ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct
     7381 gcccacaaca aaacaacgtt actgagtttt ccctgccgct ttacactgcc gttcccactc
     7441 atcggggcta taacagcttg cagaaagaac tttcaccgtg tgcagctttt cacgattaat
     7501 acagtaaagc gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact
     7561 cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct
     7621 gttattttgt ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt
     7681 gataagagtt ttcacatcaa tttacagatt aacagataaa cctattatca acggcaatgg
     7741 aatggcaaaa ttgcgaagtg aaactaatga tttcttaatc ctggcgttgc acccactctt
     7801 ttaacgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggt aaagtcagcc
     7861 agcgcaccgg ttgttgaaaa cgggccagct tatctttcac ccattcgcca agatcaacgg
     7921 tttgctggtc atactccacc accgctaccg gtcgatgacc aaactccttg tcagcaacgg
     7981 gaacgataaa cacctgcaaa accgcaggat gtgcagcaat aacgcgctcg acttcttctg
     8041 gctgaatgcc ttcgccacca ctgaagaaca gattgtctaa acgtccgaca atggtcagct
     8101 tgccattatg catctcaccg cgatcgcgcg tggcgtacca gccttcgtca ttaaccagtg
     8161 gaacccgttg cccgttacgc cagtaacctt ctgccatgct ggcagcccgc agccacactt
     8221 cgtcgtttac gattttcact tcccgacccg gcagcggcga accgacgtct gccagaccgt
     8281 cggcttcttt cgcacacacc gtggaggcaa actcggtcag accatagccg caaaagcacc
     8341 gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta
     8401 aaaccgcttt cagggaaacg gaactacggt taaccagcaa acgccagagt tgtgttggca
     8461 ccagtgaagc gtgcgtacag cctgccagca tttgctccaa tggctgttta tcacgtaccg
     8521 tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca
     8581 gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg aatcagcgat aacacccctt
     8641 cggcgctggc aagatgagcc tgataagtat gaacagcggc tttcggcaag ccagtggaac
     8701 cagaggttaa cgtcattgag cacagacgcg tcggctgcca cgccacggca tgtacgcctt
     8761 caacctgctg aatatgcagc gacgttaacg ccggaaacgt gttttcccct tccggcacca
     8821 gcgcaaattg cagcgtaaga ttgggcagca attcctcaag cagcggttgc ggcagctgag
     8881 ggttcacggg caacacccgc gccccgcatt gcagtaacgc cagccaggcc agcagcgttt
     8941 gcggcgtatt ccacgcccgc aacatcacgc cgctgcccgc aaccacaccc tgcgccgcaa
     9001 agctggaggc taattcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat
     9061 taagacgtaa ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag
     9121 agaagatcat aacagccgct ccagtgcatc aacgtccact aacggcagtg gactccccgg
     9181 ccagcgacga acctgctgca cctgcatcag atccagcgtg tccagtcccg gaatggtgtc
     9241 tggcgttaac caggcagcaa tccgcgccag ttgcattaga cctaagctcg actcaatgga
     9301 agaactgatc accgccgtca gccccagcgc atgcgccgcc ttcacctgct cgcgtacttt
     9361 ctccaggctg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagctac
     9421 aaaggcaaaa tccggctcgc gcaagctttc atcccaggca atggcaatgc cggtttcacg
     9481 ggcaaacgca cgcgaatcat cgcgggtttt gcacggctct tcgagaaatg cgatgcggtg
     9541 gcgataatcc gggttaacgt atttggcaaa ctgctggcct ttcagcggtg tccaggcgcg
     9601 attcgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat tcaccaccat
     9661 gccgtcgcgc acggcttcat acaatcccac tttgaccttc gccactttct cgccaggcat
     9721 atcggcaagt ttgaggatca gatcgtcagg atcgccatta cacagcggtg ccgcacggta
     9781 attggccgcc tgcgggagcg tatctgccag ctccgccaat gcacaactta cgccaaaggc
     9841 aacggaaggc atctgcggta attcgcaatc gcctgccagc cagttattta cccaggcaag
     9901 cagcacactt tgcgcatctt cccaggtttc ctgactgaag cccggcagtg gggagatctc
     9961 cccccaccct tcgcgctcac cctcacgcag gcaaacatac agcccgtcgc gggtttttaa
    10021 ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc
    10081 gctacgcatt acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa
    10141 gcattgcgac cttcctgacc ttcttccgtc atgtagaaca gcatggtagc gttgcccgcc
    10201 agctcctgca gccccgcctg cccgtcacag tcggcgttca gcgcagcttt caggcagcgc
    10261 agcgccatcg ggctgttttg cagcatttca cggcaccaac ggacggtttc tttttccaga
    10321 tccgccagcg gtaccacggt gtttacaagg cccatatcca gcgcctgttt cgcgtcgtac
    10381 tgacggcaca agaaccagat ttcacgcgct tttttctgtc cgacgatgcg agccatgtag
    10441 gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctggcc gaagatggca
    10501 ttatcagccg cgatagtcag gtcgcacatc atgtgcagaa cgtgaccgcc gccgatggag
    10561 tagccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc
    10621 agcacgttca ggtgatgtac gccggaatca tctttatagc cgccgtaatc gccacgcact
    10681 ttctggtcac caccggagca gaacgcttta tcgcctgcac cagtcagaat gatcacgccg
    10741 atgttgtcgt cataacgcgc atccgccagc gcctggatca tctcttttac cgtcagagga
    10801 cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat
    10861 ttttcataac gaatgtcctc aaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa
    10921 agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg
    10981 ccagacttgc gattacgcca gcgggatttt cccggtgcgc gttatgtccg gcgcgaggaa
    11041 tgacatggca gtcggcagcc agttccgccg ccagagcgcg gaatttgctg tcacgttcac
    11101 cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcgggctgga
    11161 cggcgagaga agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca
    11221 gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc
    11281 aatcggcaaa taccgccgtt aacggttctg tacgaaaacg ctgcgcccat tggcgatcgg
    11341 aacgctgacg ttccgcgcgt tgttcagcat tttgcaaccc aggatgcccg ccttcgacaa
    11401 caactccaca aagccccgcc agctcctggc aagccgccat catcgccacc cgtccgccaa
    11461 gcgagtaccc caccagccag aagttaagga tgttgtaact aaccagcgtt ttacacagta
    11521 agtcggtgac atcatcaaat ccatcgacgc tgatcgtcgc tgaaccaccg tgacctggga
    11581 gatcaacata caaccgcgag tagtcggcaa acgcctcgcc cacttcttgc cattcatggc
    11641 aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa acctggtttg ccgtgttttg
    11701 cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc
    11761 accatcggtg tcgttaacca ccatttcaat caccgtggtg gtcggcgtac gccaggcatc
    11821 gactagtgtc gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat
    11881 cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgcc cgcgctcgct
    11941 tttcggcgtt ggcaacagcg aaaaaatttg cccgccgttg ttgttcacca caattaatac
    12001 cagcggtgcg gaaacctggc gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag
    12061 atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgccgg cggcagtcga
    12121 gagcagcccg tcgataccgc tggcaccacg gttgctgtac accgggtaac ctgccggaag
    12181 ttgcgaaagc gcatcaatca gacgcaccac caggctgtta ccgacaaaca attgcccctg
    12241 ttcaggcaaa tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg
    12301 ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg
    12361 gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta agcgacgtcc
    12421 gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca acaatccagt actcttctgg
    12481 ttcacagctt gcctgccatt ggaggagtcg tttccccgtc aggctgcttc ccaattgcac
    12541 cactatttgt gcctgttgca gttcgctggt cgctttggca ttgcctaacc agagatcggc
    12601 acacggcagc ggctgtccgg tttgcgacag cacgtcgcca atcagcggcc agccaagagt
    12661 ttgcgcccac agtgcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac
    12721 accgcgcttt tgtcgccaga agaaccagtc gcgctgtttt tcactttccc gacgaggcgc
    12781 ttcacgcagc cacggtttgt cgtcctgcca ccagtcgccc agccgctgtt gccagctaat
    12841 ccccgtgtcg tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc
    12901 accagcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat
    12961 atcctgggtc gggcgcggca gtgaaatact gtgcgtgggg tgagaggcga acattcccgg
    13021 ctgacgaatc gcctgattcg cgccgcagtc aatcagctcc ggcgggcgat cggcggttaa
    13081 caggatcagt ttttctccgg ttaacccagc ttcaatcagc gccggataga gatttgctac
    13141 cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag
    13201 cgccagatgc cccagtccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc
    13261 cgccgccgct aacgttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtctgac
    13321 gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac
    13381 tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat
    13441 tattgatatg aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca
    13501 gccctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa
    13561 tgcctgcgcc agcatatagc cgcacgatat tgccgctgat ttttgctgag cgcagggaaa
    13621 cgcagaattc actttgctgt aatgaaagat agcccgcaga accggcgtac cattctcgag
    13681 tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg
    13741 ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc
    13801 agatacagcg gcgaagatgc tgtactttac gtagacgcag tacctgcggc ggtaaaacat
    13861 ccagcgtccg ggtatcggcc tgcaatcgtt ggcagatatc ttccaccacc aacatgttct
    13921 cgcgctggtt tttatcatcc gccatcagcc actcacctaa ctgctgcgcc tgcttatcat
    13981 caggatgatt tgctaccgtt cccgccagcg cttcggtacg cagcgctttg tcacgccgcc
    14041 gccataaccg ttccggtgaa gaaccaagaa aagcattttc gccatcaaag gccatgtaaa
    14101 aatggtagca attcagattg agccgacgac tggcagccat catcgctgcc gcattgaccg
    14161 gacttgcgaa atgcaggtca gttgcccgag caagcacaac tttgtcgagc tcgccttcgg
    14221 cgatggtttt cgttgccagt tcgattaata gcgtccagcc cgttttgtcc ggccagtgtt
    14281 gttctcgcgt ggtggttaaa tgtaatccag gcaggggctt gatactcacc agtgtggcga
    14341 taaattcttt tgccttgatt gcatcgtgct gaagggagct ttcgctgaat agcgtcaacc
    14401 gcagcgtggc tttaccgcca caacgtcgcc attccaggcg tggtaaaagt aaatagccct
    14461 gcgacgggtc aaacgcattc aacccccaga tgcgtaagtc ggcgtgttcc gggtgctggc
    14521 gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcaccc aggacggcag
    14581 cttcttcatc accattacgt tgttgccagt agaattgcgg atacacctgc tgactggcca
    14641 gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc
    14701 cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa
    14761 gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt acgacaaaaa
    14821 aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagaacca
    14881 gcccaacggc cgcacccaca ccaattcctt gccagggttt ttcgtggacg tagtcatcag
    14941 cacgataaac tgcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt
    15001 ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg
    15061 cgggatcgcc agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt
    15121 catcaatacg tgtatcacca aactgattag acattctcgt tctccatttg tgtaaaacct
    15181 gtcactaact atagacaagg gttattgatt acgcctgaat tacctcgcgt gccatcccaa
    15241 tgtgtgggat accatcttct tcataaatat cggtcacagg gatgaaacca aattgcccat
    15301 aaaagttttg taaatgcgcc tgcgccccca gataaatcgg cttctccggc cagcggcgag
    15361 tacacgattc cagcgcctta ctcatcaatt gctgaccgat tttttcaccg cgtaaggctt
    15421 cgctgacaat cacccgacca atcacaacgg gttgcagttc gtcgtcactt ttcagaatcc
    15481 tcgcatacgc caccagtgtc ccgttatgcc aaccgagaat gtggcgattt tctccctcca
    15541 gatcgtcacc atcgatatcc tggtaaggac agttttgttc cactacaaat accgcacaac
    15601 gcagttgtaa taaggcataa agctgagaaa cggataaatc agaatgatgc agatcttgcc
    15661 agtcgatcat ggaaggctcc tttgtgctgt ttgctttata ctagccggaa tattttcagc
    15721 agcgtgatga actcatggaa ttaatttttt taggtacttc agccggtgtg ccaacccgta
    15781 cgcgtaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt
    15841 tatttgactg cggtgaaggc acccagcatc agctactgca tactgccttt aaccctggaa
    15901 aactggacaa gatttttatc agtcaccttc atggcgatca tctctttggt ttacccggct
    15961 tgctgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcacg
    16021 gtatccgtga atttgtggag accgcgctgc ggattagcgg ctcatggacc gattatccgc
    16081 tggaaattgt cgaaattagc actggcgaaa ttttcgatga tggcctgcgc aaagtgaccg
    16141 cttatccgat ggaacaccca ctggaatgtt atggttatcg tattgaagaa catgataaac
    16201 caggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgcctggc ccgctgtttc
    16261 aggaattaaa agcgggcaaa acaatcatgc tggatgatgg aaggcagatt aatggcgcag
    16321 attacttagc cgctccagtg ccgggtaaag cgctcgctat tttcggcgat accggcccct
    16381 gcgatgccgc gctggagctt gctaaaggcg tcgacgtaat ggttcacgaa gcgactctgg
    16441 atatggcgat ggaggccaaa gccaacagtc gcggccatag ttctacacgc caggcggcgg
    16501 cactagcccg tgaggctgga gtcggtaagc taatcattac ccacgtcagc tcacgctatg
    16561 atgacaaagg ttgtcagcac ctgttacgtg aatgtcggtc aattttcccg gcgactgaac
    16621 tggcgaatga tttcgcggtg tttagcattt aagtcgaaag cggtggttag ttagcagatt
    16681 aatttaacga taccaacaca tttatttgac attttttcat ctgtttgccg gatacgtttg
    16741 atgtcgatat caggcaaaca gaattatcat ttttagtttt gtgaattgtc cagatctttc
    16801 gccagtccaa tcaggcgaat aaattccgct ctgtaaccct gtggatcttc cccttttgcc
    16861 ttctgagccc actgtttgat ctgctgccag gaggtattgt tcaggtattc agaaccgcgt
    16921 aacttttgcc catatgcagc cactgctgcg cgaaagcgca tatcttctga aggtgccttt
    16981 attgcagatg ccaacgggaa ttcaactaac tgactttctt ttccctgtgg ggatttccaa
    17041 cgaatcttta accaggcaag ttcttttgtt ttgtccgatt tcgctgattt gttatccggg
    17101 gcatagcgta acttatcaat tgatgctttt tgccctttca gcgttaattc aaataacaat
    17161 gttatatgtt tacctgcgcc aatatcaccc gcatcaacgt tgtcgttatt aaaatcctct
    17221 gccttaagtt ggcgcttttc ataacctatc tgacggtatt ccgttaccca cgcgggatta
    17281 aactcaattt gcgctttgac atcttttgct acggtaatca acgtctgccg catttcactg
    17341 ttcaacactt tctgcgcctc tgagagggta tcaatgtagc tatagttgcc gttaccaaca
    17401 tcggcaattc tcaccatcat tgcctcgtta taattgctat cccccacgcc aagcgtcgac
    17461 agagtaacac cggactcacg ctgttttttg accattgatt caatcgattt tggatcgtca
    17521 atgccaacgt taaagtcacc gtcagtggcc aataaaatgc gattgatacc acctttaata
    17581 aaccccttcg ctgcctgctg ataagccatc tccagcccgg caccgccatt ggtactgcct
    17641 tctgcatcca gcgaatcaat tgcagcatta atttccgcct tgtgactacc ggagatagaa
    17701 ggcaacgcaa tacgagagtc gccagcgtag gtcacgatgg caatgttatc ctgctcacga
    17761 agttctttga ccaataattt caacgaagac tggataagtg gcaaacgttc atcagaaatc
    17821 attgaaccag acgtgtcgat aagaaagacc agattagaag cgggtaactc ttcacttttg
    17881 cgatctttcg ccaggatatc aactttcagc aatgttcgct gttcattcca gggtgcaggt
    17941 gccaattcgt agcgcatagc gaaaggtatt ggcttagagg ccggaataga ttgtttgtct
    18001 ttgatatccc agtcagacgg gaaataattg actatctcct ccacccgcac agcgtctggc
    18061 ggaggtaaca gcccttgatt aaggaaacgc cgcacattcg cataactgcc ggtatcgacg
    18121 tcaaggctaa acgtcgccaa cgggttttgc gctacctgct taaccggatt atcatcgaac
    18181 tgctggtagc gagcggttgc tggatttgcg atttgggagg ctgctttttc tctggctgca
    18241 tgttgatatt ttggagccgc ctgcaatcgc cccagcaaag cctgtttgtc tgaatattgt
    18301 tgcacttctt gctgggctaa ggctttcgcg tctgcagcgg ctttggcggc gctttgctca
    18361 gccacttttg ttgccgcttg ttgcgcggca agcacttgct ggtcagtggg tgtagtagat
    18421 tgttgttgct gacttttctt atcctcaggt tccggcccac atcctgacaa a
//
LOCUS       DACADA010000082        18336 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.083, whole genome shotgun sequence.
ACCESSION   DACADA010000082 DACADA010000000
VERSION     DACADA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.083"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            41..115
                     /gene="tnaC"
                     /locus_tag="ISB17_002854"
     CDS             41..115
                     /gene="tnaC"
                     /locus_tag="ISB17_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAP1980760.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            336..1751
                     /gene="tnaA"
                     /locus_tag="ISB17_002855"
     CDS             336..1751
                     /gene="tnaA"
                     /locus_tag="ISB17_002855"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAP1980761.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1843..3090
                     /gene="tnaB"
                     /locus_tag="ISB17_002856"
     CDS             1843..3090
                     /gene="tnaB"
                     /locus_tag="ISB17_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAP1980762.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3222..4397
                     /gene="mdtL"
                     /locus_tag="ISB17_002857"
     CDS             3222..4397
                     /gene="mdtL"
                     /locus_tag="ISB17_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAP1980763.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            4372..5331
                     /gene="yidZ"
                     /locus_tag="ISB17_002858"
     CDS             4372..5331
                     /gene="yidZ"
                     /locus_tag="ISB17_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAP1980764.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5488..6237
                     /locus_tag="ISB17_002859"
     CDS             5488..6237
                     /locus_tag="ISB17_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980765.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6259..6825
                     /gene="yieF"
                     /locus_tag="ISB17_002860"
     CDS             6259..6825
                     /gene="yieF"
                     /locus_tag="ISB17_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAP1980766.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6878..8215)
                     /locus_tag="ISB17_002861"
     CDS             complement(6878..8215)
                     /locus_tag="ISB17_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1980767.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8382..9047
                     /gene="yieH"
                     /locus_tag="ISB17_002862"
     CDS             8382..9047
                     /gene="yieH"
                     /locus_tag="ISB17_002862"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAP1980768.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9114..9581
                     /locus_tag="ISB17_002863"
     CDS             9114..9581
                     /locus_tag="ISB17_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980769.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9630..10217
                     /locus_tag="ISB17_002864"
     CDS             9630..10217
                     /locus_tag="ISB17_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980770.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10278..11000)
                     /locus_tag="ISB17_002865"
     CDS             complement(10278..11000)
                     /locus_tag="ISB17_002865"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP1980771.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11015..12184)
                     /locus_tag="ISB17_002866"
     CDS             complement(11015..12184)
                     /locus_tag="ISB17_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP1980772.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12211..13827)
                     /locus_tag="ISB17_002867"
     CDS             complement(12211..13827)
                     /locus_tag="ISB17_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP1980773.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13913..15307)
                     /locus_tag="ISB17_002868"
     CDS             complement(13913..15307)
                     /locus_tag="ISB17_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP1980774.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15326..17200)
                     /gene="bglF"
                     /locus_tag="ISB17_002869"
     CDS             complement(15326..17200)
                     /gene="bglF"
                     /locus_tag="ISB17_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAP1980775.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(17333..18169)
                     /gene="bglG"
                     /locus_tag="ISB17_002870"
                     /pseudo
     CDS             complement(17333..18169)
                     /gene="bglG"
                     /locus_tag="ISB17_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
BASE COUNT         4660 a         4548 c         4295 g         4833 t
ORIGIN      
        1 attatactgt agcctcgtgt ctttcgagga taagtgcatt atgaatatct tacatatatg
       61 tgtgacctca aaatggttta atattgacaa caagattgtc gatcaccgcc cttgatttgc
      121 ccttctgtag ccatcaccag agccaaaccg attagattca atgtgatcga ttagtttgct
      181 atatcttaat tttgcctttt acaaaggcca tctctcgttt atttacttgt tttagtaaat
      241 gatggtgctt gcatatatat ctggcgaatt aatggatatt gcaaatgtaa tattcacagg
      301 gatcactgta attaaaataa atgaaggatt atgtaatgga aaactttaaa catctccctg
      361 aaccgttccg cattcgtgtt attgagccag taaaacgtac cactcgcgct tatcgtgaag
      421 aggcaattat taaatccggt atgaacccgt tcctgctgga tagcgaagat gtgtttatcg
      481 atttactgac cgacagcggc accggggcgg tgacgcagag tatgcaggcc gcgatgatgc
      541 gtggcgacga agcctacagc ggcagccgca gctactacgc gttagccgag tcagtgaaaa
      601 atatctttgg ttatcaatac actattccga ctcaccaggg ccgtggtgca gagcaaatct
      661 atattccggt actgattaaa aaacgcgagc aggaaaaagg cctggatcgc agcaaaatgg
      721 tggcgttctc taactatttc tttgatacca cgcagggcca tagccagatt aacggctgta
      781 ccgtgcgtaa cgtctatatc aaagaagcct tcgatactgg cgtgcgttac gactttaaag
      841 gcaactttga ccttgaggga ttagaacgcg gtattgaaga agttggcccg aataacgtgc
      901 cgtatattgt tgcaaccatc accagtaact ccgcaggtgg tcagccggtt tcactggcaa
      961 acttaaaagc gatgtacagc atcgcgaaga aatacgatat tccggtggtc atggactccg
     1021 cacgctttgc cgaaaacgcc tatttcatca agcagcgtga agcagaatac aaagactgga
     1081 ccatcgagca gatcacccgc gaaacctaca aatatgccga tatgctggcg atgtccgcca
     1141 agaaagatgc gatggtgccg atgggcggcc tgctgtgcat gaaagacaac agcttctttg
     1201 atgtgtatac cgaatgcaga accctttgcg tggtgcagga aggcttcccg acatatggcg
     1261 gcctggaagg cggcgctatg gagcgtctgg cggtaggtct gtatgacggc atgaatctgg
     1321 actggctggc ttatcgtatc gcgcaggtgc agtatctggt cgatggtctg gaagagattg
     1381 gcgttgtctg tcagcaggcg ggcggtcacg cggcattcgt tgatgcaggt aaactgttgc
     1441 cgcatatccc ggcagatcag ttcccggcac aggcgctggc ctgcgagctg tataaagtcg
     1501 ccggtatccg tgcggtagaa attggctctt tcctgttagg ccgcgatccg aaaaccggta
     1561 aacaactgcc atgcccggct gaactgctgc gtttaaccat tccgcgcgca acatatactc
     1621 aaacacatat ggacttcatt attgaagcct ttaaacatgt gaaagagaac gcggcgaata
     1681 ttaaagggtt aacctttacc tacgaaccga aagtattgcg tcacttcacc gcaaaactga
     1741 aagaagttta attaaaacac tcagagtggc tataaggatg ttagccactc tattacccta
     1801 catcctcaat aacaaaaata gccttcctct aaaggtggca tcatgactga tcaatcagaa
     1861 aaaaaacact ctgcattttg gggtgttatg gttatagccg gtacagtaat tggcggtggg
     1921 atgtttgctt tacctgttga ccttgccgga gcctggtttt tttggggcgc ttttattctt
     1981 attatcgcct ggttttcaat gttacattca gggctattat tattagaagc aaacttaaat
     2041 tatcccgtcg gttccagttt taacaccatc accaaagatt taatcggtaa cacctggaac
     2101 ataatcagcg gtattaccgt tgccttcgtt ctctatatcc tcacttatgc ctatatctct
     2161 gctaatggtg cgatcattag tgaaacgata tcaatgaatt tgggctatca cgctaatcca
     2221 cgtattgtcg ggatctgcac tgccattttc gttgccagcg tattgtggat aagttcatta
     2281 gccgccagtc gtattacctc attgttcctt gggctgaaga ttatctcctt tgtaattgtt
     2341 tttggttctt tcttcttcca ggtcgattac tccattctgc gcgattccac cagcaccact
     2401 gcggggacgt cttacttccc gtatatcttt atggctttgc cggtgtgtct ggcgtcattt
     2461 ggtttccacg gcaatattcc cagcctgatt atttgctatg gcaaacgcaa agataagctc
     2521 atcaaaagcg tggtcttcgg ttcgctgctg gcgctggtga tttatctctt ctggctctat
     2581 tgcacgatgg ggaatatccc gcgagaaagc tttaaggcga taatctcctc aggcggcaac
     2641 gttgattcgc tggtgaaatc gttcctcggc accaaacagc acggcattat cgaattttgc
     2701 ctgctggtgt tctctaactt agctgttgcc agctcgtttt ttggtgtcac gctgggattg
     2761 ttcgattatc tggcggatct gtttaagatt gataactccc acgccgggcg tttcaaaacc
     2821 gtgctgttaa ccttcctgcc acccgcgttg ttgtatctga tcttcccgaa cggctttatt
     2881 tacgggatcg gcggtgccgg actgtgcgcc accatctggg cggtaataat tcctgctgta
     2941 ctggcaatca aagcccgcaa gaagtttccc aatcagatgt tcacggtctg gggcggcaat
     3001 cttattccgg cgattgtcat tctctttggc ataaccgtaa ttttatgctg gttcggtaac
     3061 gtctttaacg tgttacctaa atttggctaa atctttcagg aagccagcca ttcgctggct
     3121 tcttgcctgt caggaaatct cttttgtcca aatggcaact cgcctgatta tccttcacca
     3181 cgtatgcttt gcgtcacctt actatcagga cgctttagcc catgtcccgc tttttgattt
     3241 gtagttttgc cctggtttta ctttatcccg cagggattga tatgtacctc gttggtttac
     3301 cgcgcatcgc cgccgatctc aatgccagcg aagcgcagtt gcatattgcg ttctccgttt
     3361 atctggcggg gatggcggct gcgatgttat ttgccggtaa agtggccgat cgttcaggga
     3421 gaaagcctgt cgccataccc ggcgcggcgc tatttattat cgcctcggtg ttctgttcac
     3481 tggctgaaac cagcgcgtta tttcttgcag ggcgatttct acagggactg ggcgcgggct
     3541 gttgttacgt agtggcgttc gccattttgc gcgacacgct ggatgatcga cgtcgggcta
     3601 aagtgctgtc gttactcaac ggcattacct gcatcattcc ggtgttagcg ccggtgctcg
     3661 gacacctgat tatgcttaaa ttcccttggc agagtctgtt ctggacgatg gcaacgatgg
     3721 gcatcgcggt actgatgttg tctttgttta ttttaaaaga aacgcgccct gcggcccccg
     3781 cagcttcgga caaaccacga gaaaatagcg agtcactgct taaccggttt ttcctcagcc
     3841 gtgttgttat caccaccctc agcgtttcgg tgatcctcac cttcgtcaac acgtcgccag
     3901 tattgctgat ggaaatcatg ggttttgagc gcggagaata cgccaccatt atggcgttga
     3961 ccgctggcgt cagcatgacc gtttcattct ccacgccgtt tgcgctggga atttttaagc
     4021 cacgtacgtt gatgatcacc tcacaggtgt tattcctggc ggcggggatc acccttgccg
     4081 tttcaccttc ccatgcggtt tcgctgtttg gtatcacgct gatttgcgcc ggtttctcgg
     4141 taggttttgg cgtagcgatg agtcaggcgt tggggccgtt ttcattacgc gcgggcgtag
     4201 ccagctcgac cttaggtatt gcgcaagttt gcggttcgtc actgtggatt tggctggcgg
     4261 cggtggttgg tatcggtgca tggaatatgc tgatcgggat tctgattgcc tgtagcatag
     4321 tgagcctgtt gctgattatg ttcgtcgcgc ctggacgccc ggttgccgct catgaagaaa
     4381 tccatcacaa cgcttgatct caatctgctg ctctgtctgc aactgctgat gcaggagcgc
     4441 agcgtgacca aagcggcgaa gcggatgaac gtgacccctt cgtcggtgag taagtcgctg
     4501 gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga actcaccgct ggggctgtcg
     4561 cccacaccgc taatggtcag catggagcaa aatctggcgg agtggatgca aatgagcaat
     4621 cagttgctgg acaaaccact ccacgaaacg ccgcgcggtc ttaagttcga actggcggcg
     4681 gaatcgccgc tgatgatgat catgcttaat gcgctgtcga aacggatcta ccaacgatac
     4741 ccgcaggcga ccatcaaatt acgtaactgg gattacgatt ccttagatgc cattactcgt
     4801 ggtgaagtgg atattggttt ttccggtcgc gaaagtcatc cccgttcgcg ggagctgtta
     4861 agctcgctac cgttatccat tgattatgaa gtgctgttta gtgatgtgcc ctgcgtctgg
     4921 ttacgcaaag atcatccggc actgcatgaa gcatggaatc tggacacctt tttacgttat
     4981 ccgcatatca gcatttgctg ggaacagagc gatacctggg cgctggacaa tgtgttacag
     5041 gagctggggc gcgaacgcac gattgctatg agcctgccgg aattcgagca gtcactgttt
     5101 atggcagcac agcccgacaa tctgctactg gcgaccgcgc cgcgctactg ccagtactac
     5161 aatcaactcc accaactgcc gttggttgct cttcctctcc cgtttgatga aagccagcaa
     5221 aaaaagctgg aagtcccttt tacccttctg tggcataaac ggaacagccg taatccgaag
     5281 atcgtctggt tacgggaaac cattaaaaat ctttacgcgt cgatggcata accgaatcgt
     5341 atgaaacgcg acccaatttc acaatgaaat gtaaaaaagt tgtaataaat ttatctgaat
     5401 cgaactttta gccgctttag tctgtccatc attccagtaa atgattactc ttgtattcat
     5461 aatggaccat taagcatgga gcgaaaaatg gcgactcact ttgcccgagg gattttaacg
     5521 gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt
     5581 ccccctcatc gtcaaagccg ttttctggcg tccagaggtt tactcgcaga actgatgttc
     5641 atgctgtatg gcattggcga attgccggaa atcgtcaccc tgccgaaagg aaaaccggtt
     5701 ttcagcgata aaaatttgcc ttcgttttct atttcctatg ccgggaatat ggtcggcgtg
     5761 gcgttaacaa ccgaaggtga atgtggcctc gatatggaac tacagcgtgc gacacgcggg
     5821 tttcatagcc cacacgcgcc cgataaccac accttttcca gcaatgaatc gctatggatc
     5881 agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca tcacgctgcg ccggagcgtg
     5941 ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg atctgcaact actgcccatt
     6001 gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag cgttatgcga cgcggaggac
     6061 gtgctggtgt ggtccgtggc ggtcacgccc acgattgaaa agctcagtgt ctgggagtta
     6121 gatggcaaac acggctggaa aagcctgccc gatattcaca gccgcgccaa caatcctacc
     6181 agccggatga tgcgttttgc ccagctctct accgtgaagg ctttttcgcc aaattgatag
     6241 acaaccacag gagtcatcat gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg
     6301 cgcaaaggct catttaatgg catggttgca cgtaccctgc cgaaaattgc tccggcgagt
     6361 atggaagtca atgcgttgcc ttccattgcc gacattcccc tgtatgacgc tgacgtacag
     6421 caggaagacg gttttccagc aacggttgaa gctctggcgg aacagatccg tcaggctgac
     6481 ggtgtggtga tcgtcacgcc ggaatataac tactcggtac cgggtgggct gaaaaatgcc
     6541 atcgactggc tttcccgcct gccggatcaa ccgctggctg gtaaaccggt attgattcag
     6601 accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt atcacctgcg ccagattctg
     6661 gttttcctcg atgcaatggt gatgaacaag ccagaattta tgggcggcgt gattcagaac
     6721 aaagttgatc cgcaaaccgg ggaagtgatt gatcagggta cgctggacca cctgaccggg
     6781 caattgaccg catttggtga gtttattcag cgagttaaga tctaaataaa aacccgccag
     6841 caatcatgca tggcgggttt ttaacgcgct atcgatttta gtgagcgtcg ataaagacaa
     6901 tcttcaggat aaacagcagc gcaacgatga ttacgcacgg gctaaggtca cgcagacgtc
     6961 cggtaccaat cttcatcacg cagtaggaga taaagcccag cgcgatacct tcagtaatcg
     7021 agaagctgaa cggcatcatc acagcggtaa taaacgccgg aacagattca gtaagatcct
     7081 gccagttcac gcgagcaaga cttgaggtca tcaacacgcc aacgtaaatc agcgcaccag
     7141 ctgcagcgta gcctggcacc atccccgcca gcggcgacag gaagataacc agcaggaaca
     7201 gcagaccaac aaccactgcc gtcagaccgg tacgaccgcc aacggaaacg ccggaagaag
     7261 attcaatata cgccgtaaca gaagaagtac cgataaacga accggtcacc gaagagatac
     7321 tgtcgacata cagcgcctgc ttcatgcgcg ggaatttacc tttctcatca gccagacctg
     7381 ctttatcggt cacgccaatc agcgtaccgg aggagtcaaa caagttgacc agcatgaaag
     7441 agaaaatcac ccctgccagc ccaaggttaa acgacccggc taaatctaca tgacccacga
     7501 cactcattac gctcggcggc gcagaaacga tgccattata gtggacatca cccagcatcc
     7561 agcccagcag cgttgtcacc acgatggaaa ccagcaccgc cgcgtgaata ttgcgcgagg
     7621 ccagaatggc gatgatgaag aagccgagga tccccagcag cacgctgtga gaagtcagat
     7681 taccgatgct caccagcgtt tccgggttag cgacaatcac acctgcgttt ttcagcccca
     7741 tcatgccaat aaacagaccg ataccactgg tgatgcccac acgtagactt accgggatgt
     7801 tggctatcat ccagtagcga acgcggaaaa tcgtcagtaa cagcagacca atcgcgcccc
     7861 agaagattgc gcccatcccg acttgccacg gcaagcccat cgcctgtaca acgacaaaag
     7921 cgaagaacgc attcaggccc attgccggtg ccagtgcaac tggcaggtta gcaaacagcc
     7981 ccatcataat actgccgaat gcagcaatca gacaggtcgt gacgaagacg gcgctggtat
     8041 ccatgccagc aacgccaaga atttgcgggt taacaaaaac gatgtaaacc atcgtcagga
     8101 aggtggtaaa accggcgatc acttcggtcc gtgccgtcgt gccatgttcg cgcagtttaa
     8161 acacgcgttc cagcatcccc tggccagaag cctgggttgt gtgttgttga ctcattatct
     8221 atttccgaac aaggagggaa aatccgtcgc tatcgtatac caaaatgaga caatatggac
     8281 ggctgaagat aacttttttt cattgtattt gcttatacgg caacgattgc gtcatgcgaa
     8341 ttggcatgac gaaaacgtta aactaacgat aaaggaaaga catgtcccag atagaagcgg
     8401 tatttttcga ctgcgacggt acgctggtcg atagcgaggt catttgctct cgcgcctatg
     8461 tcacgatgtt cagggaattt ggcattcacg ttgacctgga agagattttt acccgtttta
     8521 aaggcgtaaa actctacgaa atcatcaaca tcatcagtaa agaacagggc gtgacgatga
     8581 tccaggccga tgccgagcac gtttaccgtg cggaagtggc gcgcctgttt gatagcgagc
     8641 ttgaggtgat tgcaggagcg aacgaattgc tggctagtgt gaaagtgccg atgtgcgtcg
     8701 tctctaacgg cccggtcagc aagatgcagc attcgcttgg taagctcaac atgctgcact
     8761 acttcccgga aaaactgttc agcggctatg atatccagcg ttggaagccc gatccggcat
     8821 taatgttcca tgcggcaaaa gcgatgaatg tgaacgttga aaactgcatt ctggtcgatg
     8881 actccagtgc cggtgcgcaa tcagggatag atgccggaat ggaagtgttt tacttctgcg
     8941 ctgacccgca caataaaccg atcgttcacc cgaaagtcac cacctttacc catctttcgc
     9001 aattaccgga actgtggaag gcgcgtggtt gggatattac ggcatagtta ttcacactcc
     9061 cttcacttac tccacttaaa ttggcacgca aaggtaagta aagggagttt gatatgtctg
     9121 tttcacgtcg ggtaattcat cacggactgt attttgcggt tttagggccg ttaattggtg
     9181 ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt tgggtgataa
     9241 tacatccaat ttttctctta ttgtcgataa ctacgggagc tattcctgcg ttgttaaccg
     9301 gtgtaatggt tgcctgtctg ccggaaaaga tcgggtcaca gaaacgttat cgttgtctgg
     9361 ctggtggcat aggtggagtt gttatcaccg agatctattg cgctgttatc atacatatta
     9421 aggtcatggc ttcctcagtg ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc
     9481 gcatcattcc tgcattgctg gcaggtgtgg tgatgagcag aatcattaca cgtctacccg
     9541 gactggatat ttcatgtcct gaaacagact ctttaagtta agcgggatgt tttatctttt
     9601 ggctactcaa aagcagacag gatgtttcta tgactcaaaa tatcaggccg ttacccctat
     9661 tcaaatatca tcccaagcca ctggaaacag gcgcatttga acaggataaa accgtagagt
     9721 gcgattgctg tgaacaacag acgtcagttt attactcggg tcccttttat tgcgttgatg
     9781 aagttgaaca tctctgtccg tggtgtattg cgaacggttc tgctgctgaa aagtttgcag
     9841 gtagttttca ggatgatgcc agcatcgaag gtgttgaatt tgagtatgat gaagaggacg
     9901 attttgccgg tattaagaac acatatcctg atgaaatgct gaaagagttg gttgaacgca
     9961 cgccaggtta tcatggatgg caacaggaat tctggctcgc gcattgtggc gatttctgtg
    10021 cttttatcgg ctatgtgggc tggaatgata taaaagatcg cctcgatgaa tttgccaacc
    10081 ttgaagaaga ttgtgagaat ttcggtatta gaaattctga tctagctaaa tgcctgcaaa
    10141 agggtggtga ttgtcagggt tatctcttcc gctgtctcca ctgcggcaag ctgagactgt
    10201 ggggcgattt ttcatagtta tttatatgat gagaacaggc cggcgcgtaa ttcacacatc
    10261 cggccttatt tttaagctta accgagcgct aattctgcgg cagcggcttt atcagcgatt
    10321 accatcagcg aaggatgcag ctgcaacacg gaagcaggaa catcttcggt aaccggacct
    10381 tgcagaacat ttttcagtgc ctgcgcttta cccgcgccac tgacaattat cagcaagttt
    10441 ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taatgaaaag
    10501 tcgccaccca actcgccatg cgccacaata tcaaccatct ctccctggat cgggaactcc
    10561 acggtttgct catggaaatg ggtcgtattt ggcaaattgc cacaaaaatg accatctaca
    10621 cctaatccca acaccaccaa atccaggcca ccttcacgcg ccagtttctg atcatgctcg
    10681 cggtagttat caatagtgag cttctggata ttctcttctt tgatccctgc aggggtgaaa
    10741 aacagattac gcagattggt aatcgttacg ccttcccctt ctttgccacg aaatggaatt
    10801 tcatcgaaat tgtagaaata gcagttatcg taccagggct tacctttaac cagagtgatg
    10861 agatattcgt acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacgcgacgc
    10921 atctttgaca tataaccaag cagatgatgt gcggcgacac ggctcatttc ctggtaatct
    10981 tcggtaatga tcaatttcat cttaatgcct tattttattt aaataatttc tgtacaaagg
    11041 ctgcataggc tggtcgccag acatccattt catggttcag accgggatat tcctggtaat
    11101 caaactttat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
    11161 cgacatcttt atcgcccacg accacagtaa aattacgtag ttgctggtta atagctgccg
    11221 gatcgttcag ccgggcagcg accccttcat ctggtacggt cgtcgtggta acaccactga
    11281 atgtggccag ccagccaaag ctttccagat gattaattcc cgataccagt gcctgatacc
    11341 caccttgtga aagccctgcc agtgcgcgac catcggcatc tttacggaca ttaaaacgct
    11401 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcatttaacg
    11461 gataaaagac tttacgtctc tcctgaggaa cgaaatcttc gggaataatg cccttcgcat
    11521 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
    11581 tatccatgat ttgcgggata cgcccctgat caatagcgga acgtccggta tcaccaaagc
    11641 cgtgatagaa atagagcact ggcaaaggct cgcctatgcc ggtgtatcct ggcggggtcc
    11701 agacatacat ctgacgttcg gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
    11761 caccatgcac aacagaacgt gtatccagat aactgcccgg taccagaatc atactggagt
    11821 taacctgacg ctgaggctta gtcattgccg tgcctgtatc aatgctgcgt acaccatcaa
    11881 cattgaaaaa atactcgtac agattgcctt tcaggacagg cgtgcgccac gaccagactc
    11941 ccgcttcatc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacgg
    12001 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gtattgacct
    12061 gagtcacata ctgtttcacc ggaatagtgg gtgccgggct ggctggcata tccgctgcta
    12121 tggcccattg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
    12181 tcatcgtaat attcctttta ttaagttcca ttaccaccag atttcagcct gggcaccgac
    12241 agcaaactgg tcgtctttat tgtcttcaaa ggtgaagcca tccagttcgt tttccagagc
    12301 tttgatatat gtagcgtaga aacgaatttc cgggcgcgag gtcaacatac tggtattgac
    12361 tttaaaggta tggaagagcg tggttttata accagactca ttatatttat tgctattcgc
    12421 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
    12481 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagaatgtg cgcctgtttc
    12541 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa tgaaatgatc
    12601 gccgatataa gcttcgccct gcgaagtcag acgaactgca gttccgcctg tgtgatcacc
    12661 ataataacga ccattaaagg tagtaaatgg acttgcacca gcataacgac caaagttact
    12721 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccaccct tatcaaattt
    12781 ctgcgttaaa gatgtaccaa acatccaggt atctttccag tcataatacc cgttattatc
    12841 ctgattatct ttttccgatg cgctttcatt tgctgagaca taacgaccgc tcaccattaa
    12901 ggtggcttta tcccataacg ggatatcttt atagcgtaaa tcaatggtat tggtattaat
    12961 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcacgaa ccagcgcgat
    13021 atcaatttta cccggaccga ctttccagtt ttccagacct acacctgcgg cagcatcggt
    13081 acgctgcgtt ttccagtcaa gcatctggat ttcaattttc ggcgcaccgt gtttacccac
    13141 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcaga
    13201 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccagtag agtactgctg
    13261 acccacgtta ccatccatca tcacgatggc atcaacccgt ttgccgtttt cgttgtagac
    13321 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
    13381 accaattgcc catgatttag gtgaaccatg ggaggctgtg ccccaaccag aacggtagta
    13441 gccgttgtaa ctaaagccga tttcatcttt cacaaattta ctgaaatctt tcagagtcat
    13501 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttaa ccagtacagc
    13561 atcaggcttt gcggcacgac tggtcgggaa tggattggcg gcataccctt gatcattcgc
    13621 acttacgcta cgattcaccg tcgctggcgt atactttttc ttctcttcat ctttatattt
    13681 tttcagttcg ctttgcgttt ctcttaacgc cttttctaat agctcaaggc gttgttccac
    13741 ggttaatgat tcagccaatg attgtgcgga aaaggctaac ggtgccatta ataaaatggc
    13801 agaggtaata atatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
    13861 gcatcgcatc cagactgttc tgaatgcgac gatatttaag gtgctttatt ggttattttt
    13921 ttaatgatag cccccgagtt ttgatcactt ctgcatacca tccgaagctt ttcttgcgcg
    13981 ttcttgtgag gcttccttca ccattatcat cacgatccac ataaataaag ccgtagcgct
    14041 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
    14101 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatca tttaaatagg
    14161 caattcgata atcgtcctgt atcgaaccat ccacttcaac gctgtctttt gcgcctaatc
    14221 cgttctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaatcc
    14281 gtaatccaac cggatcaatt tgccaccccc actctgaact tttcagatgc ggatttggga
    14341 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
    14401 cggtcatgta ataactaaaa gagatgaaat ctacggtatg ttttaaatct tctgcgtcac
    14461 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
    14521 actgaccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
    14581 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
    14641 caattttcgc ttcggggatc aggctatgac aggctttaac tgcccgcgca ctggcaacca
    14701 gttgatgatg gatcgcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
    14761 ccgtgaatgg cgcgtgtaat gacatgttga tttcattaaa tgtcagccat aacacaactt
    14821 tatgttggta gcgggtaaag accgtgcgtg cgtaatgctc gaagtgatcg atgaccgccc
    14881 gattagccca accgccgtag tttttcacca gcccatatgg catttcgtaa tgggataacg
    14941 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgctcg taaaacgcta
    15001 accccgcttc attcggttcg gcttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    15061 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    15121 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    15181 ttcccattac gccatgaggc tgtaaatctg aggtcgtgat ccctttgcca tcttcctgcc
    15241 atgcgccttc cacctgattg gcagctgttg caccacccca aagaaatgtt tctggaaatg
    15301 ctttcataat taactccttt tatcgttagc gaatgatgga taacagcggc tcaccagcgt
    15361 ttatctgcac cgtgccgtgg ggtaatacgt ccgtaaaatc atcgctatta ctgattaata
    15421 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    15481 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    15541 gttttacggt gtcgataccg acatgaatca ggatctccac gccatcatct gactcaatgc
    15601 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgccaccgga gaacgcactt
    15661 caccaacaga gggcagaatg gcaatacctt tacccaacag gccactggca aacgtggtat
    15721 cagcgacgtg aatgagcggc acaatatctc ccgtcatcgg tgaacagata ccgccctgct
    15781 caggtgtaat aatctctggt gttttctctt gctgggcaac ttgcgctggc tgacgtttag
    15841 cggtgatgaa atgaagcatc accgtaccga caaatgcgca accgatggca atgacaccac
    15901 caataacgct ggcccatacg gtgaaatcaa tccccgttga cgggatggtt tgcatgaagg
    15961 tgaaaatacc tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg
    16021 cccccaatgc cccacttatg caggcgataa cgaaggggta cttacgcggc aggttgacgc
    16081 catatactgc cggttcggtg ataccaaaca gccccgtcaa cgccgccgat cccgctacca
    16141 cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acttgcgcca
    16201 taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga
    16261 taattaaagg tatcaaaccc caatgcagac caaacatcac aaagatttgc cagaagccgc
    16321 ccattaccgc gcctgcaaat gcaggaaccg cctgataaag ccagagataa ccggcagcaa
    16381 tcagttcact tatccaggtt gatagcggcc ccaccagcag aaatgtgatg ggcgtgatga
    16441 ccatcagaca tagcaagggt gtgaagaaat ttttgattgc cgaaggtaac cacgcattaa
    16501 gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg
    16561 agtaattcaa caatgtgacc ggaataccca ggaaatccag gcccagcgca tccacttttt
    16621 gcccgttctc gaaggcagtc agaattaatg gatgcactaa cgctccgcca atcaccatgg
    16681 cagtaaatgg attgccgctg aagcgtttcc ctgcggtgta gcccaggatt atcgggaaga
    16741 accaaaacaa ggcatcactg gcgctaaata aaattaaata agtaccactt tgttcggtcg
    16801 tccactgaaa agtgagtgcc agagccaaca tacctttcag gatcccggtt gccgccatca
    16861 aaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt aacagattac
    16921 ctttatcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa
    16981 ccgccaggaa gacatcggcc acatggttac ctatgacaac ctgaaactgg ccaccgcttt
    17041 ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat
    17101 cctttaactt aaaacgtaat cgcgttgcac aatgcatcag actcacaatg ttatctgcgc
    17161 ccccgactcc tgcgactatt gttctggcta actccgtcat aacttgccct ctaccgcttt
    17221 gcggcaaaac tcaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
    17281 tcaggttttg cccgcttagt gcggtaacaa tcctttattc agtaataata tttcagtgtt
    17341 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcaggt gagattttac
    17401 gttgatactg caaaccaata aaaatggcga tccgctccgc acattgccat gcttgcgggt
    17461 aattttgctt tactgcttat tgtaatgatt catcactatc gttaatcgat gcatgttcaa
    17521 taatacgcca ggataaaaat ttcagatgcg taaccagtcg ctgataactc aagctttctt
    17581 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttctcgc attaattgcg
    17641 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
    17701 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
    17761 cttcttcccc tagctggaac tctttcgggt aaagtcgctg gatatcccac agcagcgggt
    17821 tgggcagtag cacgttttgc tgaaagcgtt taatcgcaaa ctggcaatgg tcagttagcg
    17881 agatataaat actgtcctgc aactttccca gacgctcctg cgccagagag ataatacgat
    17941 cacaggttgc catcacctca agaggcatat gacttaagag ttcgcttaat cgcccgttca
    18001 gttcatgact gctcaaggca tactcttttt ctattccact tgagttaatt ctttcgcctg
    18061 ggcgtttttg aaagccaatt ccgcgcccca tgacgacttt ttcccgctgt tgatcatcaa
    18121 taaccaccac aacattattg ttgagaattt tggtgatttg catgttcata gcaaggacct
    18181 tttttataaa caaaaaaacc cgacttcacc agtattctct ggttatgtca ggttttgcct
    18241 gcgaatgcag taacaatcca gtcattaatt aatggttttt ataacgaaca tccaggttcg
    18301 aaaattaatt tagttgcgtg cagaaaaaat agcaat
//
LOCUS       DACADA010000083        18261 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.084, whole genome shotgun sequence.
ACCESSION   DACADA010000083 DACADA010000000
VERSION     DACADA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.084"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..839
                     /locus_tag="ISB17_002871"
     CDS             <1..839
                     /locus_tag="ISB17_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAP1980776.1"
                     /translation="SFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQ
                     VQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLE
                     EKVTDALAGDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQYSVNPSESESASS
                     SDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAV
                     FFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSGGGGSSGGGGASG
                     RW"
     gene            complement(899..2197)
                     /gene="eno"
                     /locus_tag="ISB17_002872"
     CDS             complement(899..2197)
                     /gene="eno"
                     /locus_tag="ISB17_002872"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP1980777.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2285..3922)
                     /gene="pyrG"
                     /locus_tag="ISB17_002873"
     CDS             complement(2285..3922)
                     /gene="pyrG"
                     /locus_tag="ISB17_002873"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAP1980778.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4150..4941)
                     /gene="mazG"
                     /locus_tag="ISB17_002874"
     CDS             complement(4150..4941)
                     /gene="mazG"
                     /locus_tag="ISB17_002874"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAP1980779.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5037..7271)
                     /locus_tag="ISB17_002875"
     CDS             complement(5037..7271)
                     /locus_tag="ISB17_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP1980780.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7319..8620)
                     /gene="rlmD"
                     /locus_tag="ISB17_002876"
     CDS             complement(7319..8620)
                     /gene="rlmD"
                     /locus_tag="ISB17_002876"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP1980781.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8677..11433
                     /gene="barA"
                     /locus_tag="ISB17_002877"
     CDS             8677..11433
                     /gene="barA"
                     /locus_tag="ISB17_002877"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAP1980782.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11664..13004)
                     /gene="gudD"
                     /locus_tag="ISB17_002878"
     CDS             complement(11664..13004)
                     /gene="gudD"
                     /locus_tag="ISB17_002878"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP1980783.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13025..14365)
                     /locus_tag="ISB17_002879"
     CDS             complement(13025..14365)
                     /locus_tag="ISB17_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP1980784.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14367..15719)
                     /locus_tag="ISB17_002880"
     CDS             complement(14367..15719)
                     /locus_tag="ISB17_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980785.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16153..16602)
                     /locus_tag="ISB17_002881"
     CDS             complement(16153..16602)
                     /locus_tag="ISB17_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP1980786.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16620..17402)
                     /gene="truC"
                     /locus_tag="ISB17_002882"
     CDS             complement(16620..17402)
                     /gene="truC"
                     /locus_tag="ISB17_002882"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAP1980787.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17402..17731)
                     /locus_tag="ISB17_002883"
     CDS             complement(17402..17731)
                     /locus_tag="ISB17_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAP1980788.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4475 a         4945 c         4362 g         4479 t
ORIGIN      
        1 gttcctttgt tgcagcccaa ccaactattg tcccacaatt acagcaacaa gttacggatc
       61 ttacgagtag cttaaactca caagaaaaga aagaactgac gcgcaagcta gaatctattt
      121 tcaataatac ccaagtacaa attgctgtat taattattcc cacaaccaaa gacgagacaa
      181 ttgaacaata tgctgcaagg gtttttgaca attggcgttt aggagatgcc aaacgcaatg
      241 atgggatact gatcattgtt gcctggtcgg atcgcactgt ccgcatccag gtaggttatg
      301 gactggaaga aaaggtaacc gatgctctgg ctggagatat catccgtagc aacatgatac
      361 ccgcatttaa acaacaacaa ttagctaaag gattagagtt agctataatc gctttgaata
      421 accgactcac ttcacaacac caatattcgg ttaatccttc agaaagtgaa tcagcatctt
      481 ccagcgatca ttactacttt gctatttttt gggtatttgc agtgatgttc ttccctttct
      541 ggttttttca tcaaggcagt aatttttgtc gcgcatgtaa aagcagtgtc tgtatttcag
      601 caatctatct tttagattta ttcctgttct ccgataaaac tttttccatt gctgtatttt
      661 tcttcttttt tactttcacc accattatgg tctttacctg tttatgtgta cttcagaaaa
      721 aagcatctgg tagaagttat cattcagaca acagcggttc cggtggcggc tcaagtggcg
      781 gcggatttag cggcgggggc ggttcttctg gcggtggcgg cgcgtccggt cgttggtaac
      841 tcaaccataa aaaacgccag cccgaaggct ggcattttta aatcagataa agtcagactt
      901 atgcctggcc tttgatctct ttacgaccgt tgtacggtgc tttttcgccc agagcttctt
      961 cgatacgaat cagctggttg tatttagcaa cacggtcaga acggctcata gaacccgttt
     1021 tgatctggcc tgcagcagta ccaacagcca ggtcagcgat ggtagcgtct tcagtttcgc
     1081 cagaacggtg agagataact gcagtgtagc cagcgtcttt cgccatcttg attgcagcca
     1141 gagtttcggt cagagaaccg atctggttga atttgatcag gatggagtta gcgatacctt
     1201 tttcgatacc ttctttcagg atcttggtgt tggttacgaa caggtcgtca ccaaccagct
     1261 ggattttgtc gcccagaact ttggtttggt atgcgaaacc gtcccagtca gattcgtcca
     1321 gaccgtcttc gatggaaacg atcggatact gtttggtcag ttcttccagg aagtgagtga
     1381 attcttcaga ggtgaatgct ttgttgcctt cgccagccag aacgtattta ccgtctttgt
     1441 agaattcaga agctgcgcag tccatcgcca gagtgatgtc tttgcccagt tcataaccag
     1501 cagctttaac agcttcagcg ataacagcca gagcttcagc gttggaaccc aggttcggcg
     1561 catagccacc ttcgtcacca acagcagtgt tcatgccttt cgctttcaga acttttgcca
     1621 ggtgatggaa aacttcagaa cccatgcgga tggcttcttt cacagttttc gcgccaaccg
     1681 gctgaatcat gaattcctgg atatcaacgt tgttgtcagc gtgctcacca ccgttgatga
     1741 tgttcatcat cggaaccggc atagagtatt tgcccggagt gccgttcagt tcagcgatgt
     1801 gctcgtacag cggcatacct ttagcagctg cagcagcttt ggcgttagcc agagatacag
     1861 ccaggattgc gtttgcgccg aatttggatt tgttttcagt gccgtccagg tcgatcatga
     1921 tcttgtcaat gccagcctga tctttagcat ctttgccaat cagcgcctga gcgatcgggc
     1981 cgttaaccgc agcaacagct ttggttacgc ctttgcccag gaaacgggat ttgtcgccat
     2041 cgcgcagttc cagagcttca cgggaaccag tagaagcacc tgacggagca gctgccatac
     2101 cgacgaaacc accctccaga tgtacttcgg cttcaacagt cgggttacca cgggagtcga
     2161 tgatttcacg accgatgatt tttacgattt tggacattag gttttcctca agtcactagt
     2221 taaactgaaa ctccagacaa acaacgcgta cccagggtac gcgttgccgt tctaactttt
     2281 ttacttactt cgcctgacgt ttctggaact cgctggcggc ttttacaaag ccagcaaaca
     2341 gcgggtgacc atcacgtgga gtagaagtaa actccggatg gaactggcaa gccacgaacc
     2401 acggatgatt cggaacttcg atgatctcga ccaactgatc atccccggaa cggcccgcaa
     2461 cgcgcagacc tgcatcttca atctgtttca acagcatgtt gttgacttcg taacggtgac
     2521 gatgacgctc aacaattgtc ggcgcattgt acagctggcg aaccaggcta tcgtcaacca
     2581 actggcactg ctgtgcgccg agacgcatgg taccgccgag atcgctcttc tcgctacgaa
     2641 cttcaacgtt gccgttttca tcgcgccact cggtaatcag cgccacaacc gggtacttac
     2701 agtctggcac aaattccgta gagttggcgt tctccatgtt ggcaacatgg cgagcgtaat
     2761 caattaacgc cacctgcata cccaggcaaa tgcccagata aggaatattg ttctcacgcg
     2821 caaaacgcgc ggtcgtaatc atgccttcta cgccacgata gccgaaaccg ccaggtacga
     2881 ggattgcgtc cagacctttg aggatttcaa cgccgcgcgt ttcaacatct tgtgaatcga
     2941 tcagtttgat gttgacgctg acacgattct tcagcccacc gtgtttcagt gcttcgatca
     3001 cggatttgta tgcatccggc agctcaatgt atttgccgac cataccaatg gtgacttcac
     3061 ttaccggatt cgcttcttcg aagataacct gttcccattc ggacagattc gcttccgggc
     3121 agtttaagct gaatcgttta caaatataat cgtccagccc ctgagatttc aacaggcccg
     3181 gaattttata gatggaatcg acgtctttca gagaaataac cgctttttcc ggaacattac
     3241 agaacaatgc aatcttcgca cgttcgttcg ctggaacggc gcgatctgaa cgacaaatca
     3301 ggatatcagg ctggataccg attgaaagca gctcttttac agagtgctga gtcggtttgg
     3361 ttttgacttc acctgacgct gccatgtacg gcaccagcgt caggtgcata aacagggtgt
     3421 gctcacggcc aatttcaaca gccatctggc gaatcgcttc gaggaacggc agggattcga
     3481 tatcaccgac agtaccgccg atttcgacca gtactacgtc atgaccttcg ccgccttcca
     3541 gcacgcgctc tttgatagcg ttagtgatgt gcggaataac ctgtacggtt gcgccgagat
     3601 agtcaccgcg gcgttcttta cgcagaacgt cagagtagat acgacccgtg gtgaagttgt
     3661 tgcggcggct cattttggta cgaatgaaac gctcgtagtg ccccaggtcc aggtcggttt
     3721 cagcgccgtc ttcagtaacg aacacttccc cgtgttggat tgggctcata gtaccgggat
     3781 cgacgttgat gtacggatcc agtttcatga tggtcacatt gaggccacgg gcttcaagaa
     3841 tggctgcgag ggaggctgcg gcaatgcctt tacccagaga ggatacgacc ccgccggtca
     3901 caaaaatata gttcgttgtc atgctgaacc tgagaagtta ggttgaaaag acgatggaat
     3961 aaccaggacg ggaaagcagt atacccgaac atgacctgtg ccacaaactt tcattatccc
     4021 tcctcttcgc cagcgcacta ttgaaatcag gagtgagaaa atagcccctt tggggtaaat
     4081 gtttttgacg caaatcaagc gcttgtcatt taaaaaatga cacaaatcgc gcttgaccac
     4141 gtaattccct tagagatcaa tttcctgccg ttttacctgt tgccagactt cttccattgt
     4201 ttcgaggtca acacctgtca tttccagtcc acgcgcggca acaatacgct ccacttcgcg
     4261 aaaacgacgc tcgaattttt cgttcgcttt ttgcaatgcg atttccgctt ttgtccctaa
     4321 atgacgagcc agattgaccg tggcaaacag caggtcaccc atttcctcct ccagtttagc
     4381 ctggtcgaca acagcctgcc gcgcctcgta catcacctcg tcgatctctt cgtagacttt
     4441 atcgactacc ggaccaagcg tcgtccagtc gaaaccaacg ttggcgcaac gtttctggat
     4501 tttttgcgca cgcattaaag ccggtaaact acgaggaata tcgtccagcg ccgaatgctg
     4561 cgctttctgc gcgcgctctt cggttttgat ttgctcccaa cgggcaagca cttcactact
     4621 attttcggca gaactgtcag caaaaacatg cggatggcga cgctctaatt tatcgctaat
     4681 agcagcgcaa atatcattaa agtcaaagcg cccttcttcc tgagctattt gtgcgtaaaa
     4741 caccacctgg aacagcagat cgcccagttc accgcgcaga tcgtcaaaat cttcacgggc
     4801 gatggcgtcc agcacctcgt aggtttcttc aagggtgtaa ggcgcaatgg tggcaaatgt
     4861 ctgctcttta tcccacgggc agccgttttc cgggtcgcgc aggcgctgca tgatggtgag
     4921 caaacggtcg atttgattca ttgaattatc ctgaaaattg cgggtccgtc aggtgtaata
     4981 ccttcgagta aaagatatcg ctggatacgg ctgacgccat atccagctat gtaagattaa
     5041 ctcccgtgca accgacgcgc gtcgataaca tccggtacct ggttgagttt acccagcacg
     5101 cgacccagca cttgcaggtt gtaaatctca atggtcatgt cgatggtcgc cagttgctgt
     5161 ttggtgtcgc tacggctggc aacgccaagt acgttcacct tctcgttggc gagaatggtg
     5221 gtgatatcac gtaacaaccc actacgatca ttagccacca cgcggaccac cagcgaatat
     5281 ccggcggagt agctctcgcc ccataccgcg tcaacaatgc gttctggcgc atgggagcgc
     5341 agttccgcca attgttcgca atcggcgcgg tgtactgaaa taccgcgccc ctgggtaatg
     5401 aagccgacaa tctcatctcc aggaatcggc tggcagcagc gcgcgatgtg gtgcatcagg
     5461 ttaccgaccc cttctaccac cacacgaccg ttatctttac tgcggttttg cggcgtgtag
     5521 cttttttgct gaagctgttt cagcgcggcg gcgtcctgct cttcggcact cggcttatta
     5581 aattgcgatt gcaggaagtt caccatctga ttgagacgga tatccccgcc accaatcgcc
     5641 gccagcaact cgtcgacatc attgaagttg taacgcggca gcagatgttt ttctgcttct
     5701 ttcaggctga tccccagatg ttccagctcg tcgtcaagga tttgccgccc agccagaatg
     5761 tttttgtcac ggtcctgttt acggaaccag gcgtgaattt tcgaacgccc acggctggtt
     5821 gtgacgtaac cgaggtttgg gtttaaccag tcacggctgg ggttcggctg tttctgggtg
     5881 ataatttcaa tctggtcgcc catctgcagc tggtaggtga acggcacaat gcgcccgcca
     5941 atttttgccc cgatgcagcg gtgtccgaca tcactgtgga tgtggtaagc gaagtccagc
     6001 ggcgttgatc ccgcaggcaa atcaacgaca tcacctttcg gcgtaaagac gtacactcgg
     6061 tcgtcaaaga cctgactacg tacttcgtcg agcatttcgc cggaatcagc catctcttcc
     6121 tgccacgcaa tcagtttacg cagccaggca atccggtctt catgtcccga acgtgcgccg
     6181 ccagcagccg cgccctcttt atatttccag tgcgcagcaa cacccaactc tgcatcttca
     6241 tgcatctgtt tggtgcggat ttggatctca acggttttgc cacccggccc cagaaccacg
     6301 gtatgaatag actgataacc gtttggtttc gggttagcga cgtaatcgtc aaactcatcc
     6361 ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg cataacaatc ctgtaaacgc
     6421 tcggcgacaa tacgtaccgc acgcacatca aacagctcat cgaaggcgag gttctttttc
     6481 tgcattttgc gccagatgct gtagatgtgt ttcggacggc catacacttc ggctttaacg
     6541 ccttcagctt tcatctcagc gcgcagatgg ccgacaaact cttcgatata gtgttcgcgg
     6601 tcgagacggc gttcatgcaa cagttttgcg atgcgtttgt attcggtcgg gtggagataa
     6661 cggaagcagt aatcttccag ctcccatttc agttgcccaa tcccaagacg gttcgccaac
     6721 ggcgcgtaga tattggtgca ctcttttgcc gccagtacac gttcatcttc cggcgcatct
     6781 tttacttcgc gcagatgagc aatacgctcc gccagtttga tgactacgca gcgaaaatca
     6841 tcgaccatcg ccaataacat ccggcgaacg ttatcgacct gttcggagga aacagaatca
     6901 gtgtgcgtcg ctttcagctg gcggatcgcc gccatatcac gcacgccgtg aataaggtta
     6961 acgaccgact taccgacgct ctcgcgcagc acatcttcgc tgactacgtt ggcatcagcc
     7021 agagggaata gcagcgccgc ccgcagcgtg tcaatgtcca tacttaatgt cgagaggatc
     7081 tccaccatct caacaccacg ccacaataac agactggcat ccggatgccc ctgcgtctgt
     7141 tgcagacaat acgcccaggt ttcggctaag cactcacacg acttctggct ggtaataccc
     7201 agacttgcga tccatttttc cggatcaaat tcaccagcct tattgatatg tgcacttctt
     7261 accgcaacca tcgtcctctc ctttagggac cagacctgcc gaaatcggca aatcgtaact
     7321 atttaacgcg cgagaaaagt accatcgatt ccagatgtcc cgtgtgtggg aacatatcca
     7381 gcatcgccag tcgcgcaatg gtatatcctg cttttaataa cgcttcgcta tcccgagcca
     7441 gcgttgcagg gttacaggat acataaacta tacgaatagg ttccagtttt ataatttgct
     7501 gcataacacc tgcggcacct gctcgcgccg ggtccaacaa cactttatcg aagccgtttt
     7561 tcgcccacgg ctgctttgtg acatcttctt caagattttc gtgataaaac gtcacattct
     7621 gtaagccatt aagacgcgca ttctgctggc ctttttccac cagcgccgga acaccttcta
     7681 caccgaccac actggcagct tgtgtcgcca atggcagtgt aaagttgccc ataccgcaga
     7741 acagatccag tacgcgatct tcaggttcca catccagcca ttccaacgca cgcgctacca
     7801 ttttttggtt cacacccgca ttgacctgaa taaaatcgcg cgggctaaaa gttaagcgca
     7861 acccatttga gtcataccag ggcatctcac cagagacggt ttcgagtatc tcactatcgg
     7921 gggcgagata cagatccagg ccttcagaat gcgaaaagca ttccagtttt tcgcgatctg
     7981 ccgaacttag cggtgcggta tggcgcaaaa tcattagcgt gccgctggtt gcctgtacca
     8041 gttcaacatg accaagatgg cgcatagctt gtaagctgcc caggcatgcc ctgactttgg
     8101 gcagcaatgc ttctagttgg ggcactaaaa tggggcattg tttaacgtcg acaatgtcac
     8161 tggagcccgc tttgcgaaac cccatctgaa gttgctgtgt tttcggtaag tagttcagac
     8221 ttaaacgcgc gcgacggcga tagccccaag gaacatcggc gatcacttca gagacttcgt
     8281 gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg ctgtaaatcc acgctggcgt
     8341 gttgttgctg acagccgccg catacgccaa aatgaggaca gcgtggcgtt tcgcgttccg
     8401 ggctatcgct taaccggcgt acgactttag cgcgggcata ctgtttttta tcttcagtaa
     8461 cagtaacttc cgcgttttcc tgcggcaata atccggggat aaataatgtt ttgccgttat
     8521 gtcgcgccac accctgacca aaagagtcga ggtcgttgac tgaaacggtt atgatctgac
     8581 gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat tggcgagact ttctcaattt
     8641 aacagtgtga ccttaattgt cccataacgg aactccatga ccaactacag cctgcgcgca
     8701 cgcatgatga ttctgatcct ggcaccgacc gtccttattg gtttattgct gagtatcttt
     8761 ttcgtcgtgc atcgctataa cgacttgcag cgtcaactgg aagatgccgg tgccagcatt
     8821 attgagccgc ttgcagtttc tactgaatat ggcatgagtc tgcaaaatcg cgaatctatc
     8881 ggtcagttaa taagcgtact gcatcgtcgc cattctgata ttgttcgcgc gatttcggtt
     8941 tatgatgaaa ataaccgact ctttgtcacc tccaattttc atcttgatcc ctcatcaatg
     9001 cagctcggca gcaacgtgcc gtttcctcgc cagctcactg tcactcgtga cggcgatatt
     9061 atgatcctcc gcacgccgat tatttctgag agttactccc ccgacgaatc gcccagtagc
     9121 gatgccaaaa atagtcaaaa tatgttggga tatattgcgc ttgagctgga tcttaaatcg
     9181 gttcgcttgc agcaatataa agagatcttt atttccagcg tgatgatgct gttttgtatc
     9241 ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg taaccggtcc gattcgcaac
     9301 atggtgaata ccgtcgaccg catccgtcgc gggcaactcg acagccgggt ggaaggattt
     9361 atgctcggcg agctggatat gctgaaaaac ggtatcaact cgatggcaat gtcgctggcg
     9421 gcttatcacg aagagatgca acacaatatc gaccaggcga cgtccgatct gcgtgagacg
     9481 ctggagcaaa tggagattca gaacgttgag ttggatctgg cgaaaaagcg cgcccaggaa
     9541 gcggcgcgta ttaaatccga gtttctggca aatatgtcac acgagctgcg tacaccactg
     9601 aatggtgtta ttggctttac ccgcctgacg ctgaaaacag aattaacacc aacgcagcgc
     9661 gatcacctga atacgattga acgttcggca aataatttgc tggcaattat taatgatgtt
     9721 ctcgacttct cgaaactgga agccggtaag ctgattctgg aaagtattcc gttcccactg
     9781 cgcagcacgc tggatgaagt cgttaccctt ctggcacact cttcccacga taaagggctg
     9841 gaattgacgc tcaatattaa gagcgacgtc cccgataacg tgattggcga cccgctacgt
     9901 ttacagcaaa tcatcactaa cctggtgggg aacgcgatta aattcaccga gaatggcaac
     9961 atcgatattc tggtggaaaa acgtgcgctg agtaatacca aagtgcaaat tgaagtgcag
    10021 attcgggata ccggcattgg tattcctgaa cgcgatcaat cgcgcttatt ccaggccttc
    10081 agacaggctg atgccagtat ttcccgccgt catggtggca ccgggctggg gctggtgatt
    10141 acacaaaaac tggttaatga aatgggcggt gatatttcgt tccatagcct gccgaatcgt
    10201 ggttcaactt tctggttcca cattaatctc gatctgaacc cgaacattat tatcgaaggg
    10261 ccatccaccc agtgcctcgc tggtaagcgc ctggcctatg tcgaaccaaa ctccgcagca
    10321 gcacaatgca cgctggatat tttaagtgaa acgccgctgg aagtggttta tagcccaacg
    10381 ttctccgcgc tgcctcccgc gcattacgac atgatgttgt taggcatcgc ggtgaccttc
    10441 cgcgagccgc taacaatgca acatgagcga ttagcgaaag cggtatcgat gaccgatttc
    10501 ctgatgctgg cacttccttg ccatgcacaa gtcaatgctg aaaaacttaa gcaagatggt
    10561 atcggcgcgt gtctgctgaa accattaaca cctacgcgcc tgttgcctgc cctgacggaa
    10621 ttttatcatc acaaacaaaa cacgcttttg cctgtaaccg atgaaagtaa gctggcaatg
    10681 acagtcatgg cggttgatga caaccccgct aacctgaaac ttatcggcgc attgctggaa
    10741 gatatggtgc aacatgtgga actttgcgat agcgggcatc aggcagttga acgggcgaaa
    10801 cagatgccgt tcgatttaat cttaatggat attcaaatgc cggacatgga cggcattcgg
    10861 gcatgtgagc tcatccacca gctcccgcat cagcagcaaa cgccggttat cgcggtaacg
    10921 gcgcatgcga tggccgggca aaaagagaaa ctgcttggcg cggggatgag tgattatctc
    10981 gccaaaccga tcgaagaaga gcgattgcat aatttgctgt tgcgctacaa gcctggcagc
    11041 ggtatttcct ctcgcgtcgt gacgcccgaa gtcaacgaaa ttgtggtcaa cccgaatgcg
    11101 accctcgact ggcaactggc actacgccag gcagcaggaa aaaccgattt agcgcgcgat
    11161 atgctgcaaa tgttactcga tttcctgcct gaagttcgca acaaagttga ggaacagctg
    11221 gttggagaaa acccggaagg cctggttgat ttgattcata aactgcatgg cagttgcggc
    11281 tatagcggtg taccgcgtat gaagaatctc tgccaactga tcgaacaaca gctacgtagc
    11341 ggtactaaag aagaagattt ggagccggag ctgctggaac tgttggacga gatggataat
    11401 gtagcgcgcg aagccagcaa aattctcggg taatggacgt cttgtcgagt agcaaccgga
    11461 tttttatgac gctggtttca gattggtgac aagtgcctgt gtttatgccg gatgcggcgt
    11521 taacgcctta tccggcctgc aaaaccaatt aaattcaatg aattaaaaaa tgatgtagcc
    11581 ctgatacgta gcgcaacagg caattttgcg tttggcatca gtctcaaacc ggctccagat
    11641 agagccggtt ttggttttct gtcttaacgc accatgcacg gacgcttgtt atcgaacgtc
    11701 cagccgggga tcaggtactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc
    11761 tgatacagct cgtgcgcttt catcacttga tccatatcga tttctacacc cagccccggt
    11821 ttttctggca cctgaaccag accgcctttg atctctaacg gttctttggt caggcgctga
    11881 ttaccttcct gccagatcca gtgcgtatca atcgcggtga ttttacccgg tgcggcagcg
    11941 gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgttggagtg tgaaccccag
    12001 gtcaggccaa attcatgaca catttgcgcc acacgtaccg aaccttgcat cgtccagaaa
    12061 tgcggatccg caagcgggat atcaacggat tgcaacgaga gcgtatggcc catctgccgc
    12121 cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg gaactctgcc
    12181 atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc
    12241 gaacctttca ggtatgtacc gattttaatc gcttcgttca gcgaccaggc accgttagga
    12301 tcgagcgtaa tacgcgcctg cgggaaacgt tgcgccagtg ccacaataga ttctgcttct
    12361 tcttccccgg ccagtacacc gcccttcagt ttgaaatcgt tgaagccata tttttcatat
    12421 gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcgtg acgcaggcga
    12481 taccagtcac attgatcatc cggctggctt tgatacggca gcggtgtggc tttgcgatta
    12541 ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttggcc atcgcccagc
    12601 agcgatgcca cgtttacccc cagatgctgc cccaacagat ccagcattgc cgcttctatc
    12661 ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccggca
    12721 tcacgatcgg caaacgtatt acgcaccagc gtcagaacgt ttttgtattc acccagcgtt
    12781 ttacccacca ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga
    12841 atttccccta cgccagtgtg accggaatta tctttgataa tcacaatatt acgcgtgaag
    12901 aacggtgcat gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg
    12961 acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc
    13021 agaatcaatg acggccgaaa acggggcgtt tacggtcaaa ggtccagccg gggatcaggt
    13081 actgcatcgg gcctgcgtcg ttacgcgcac cgccaggcag acgtttatag gcctcatgtg
    13141 ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa
    13201 tttttccgtt tttaatctcc agcggatttt tggtcagacg acaatcgccc tcctgccaaa
    13261 tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca
    13321 tcgccagaga gatatcaaag tggttattag aatggcagcc ccaggtcagc ccccagtcgt
    13381 cgcaaagttg cgccacgcgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg
    13441 gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcaatca
    13501 tgttggtcgc gacgggcaag ccggtcgccc gtcgaaactc cgccatcact tcacgaccgg
    13561 agaaaccctg ctcagcgccg cacgggtctt ccgcataggt aagaacatca ttcagccctt
    13621 tgcacagaga aatggcttca tcaagcagcc aggcaccatt gggatcaacg gtaatccgcg
    13681 catccgggaa gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca
    13741 cgccaccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac
    13801 gcacaacggc ttcgctgttc atcgctttct gatgccgcaa ctgataccac tcatggttgc
    13861 ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata taaaacagat
    13921 aaccgagaac ggtaatagca tcacgttgct tgcctggccc taacagttcg cagaccggaa
    13981 cattcagcgc ctgacctaac aggtcaagca aggcggcttc cagcgccgct acggcattaa
    14041 cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct gcctgattac
    14101 ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc agcaccatcg
    14161 gaatagcccc gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg
    14221 tatgcccggc attatcggtg agtaccacaa tattgcgggt gaaatatgcg ttatgtgcac
    14281 caccaatatt aagcaacatg ctgtcatgtc cggcgaccgg aatgactttc atatcagtaa
    14341 taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag ctcaatacgc
    14401 ttgatatcgc ccaccagcac caggtagctg agtaccgcga ttaaggcatg aacaccaaca
    14461 taaatcagcg ccccattaaa ggagccagtc gtgccaacga tataaccaat tgcgattggc
    14521 gtgacgatgc cagaaatgtt accaaacatg ttgaacaggc caccggaaag accgctgatc
    14581 tcttttggcg cagtatcagc cattacagcc caacccagcg caccgatccc cttaccgaag
    14641 aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc
    14701 atcaccatcg acaacaacat gcccatcacg atcggtgttt tacgcgcaat gttcagtgaa
    14761 cccgtgcgac gcatcagcca gtcggaaata atccccccca acacaccgcc gataaaaccg
    14821 caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc gcgcgcctgc
    14881 accagataaa ccgggaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga
    14941 ccgatataaa cgccgatcat catccgtgac ccgagcagct gtttgatctg cccccacttc
    15001 acgctgaacg gcactttaac tttggtgttt tgctgatcca tattgatcag cgcgccaccc
    15061 gcggcgatgt actccagctc tttctgattt acgcccggat gttgatttgg ctcgtggatg
    15121 actttcaacc agataaagct gatgacaatg cccagaccgc ccataaagaa gaagacgtgt
    15181 gaccagccca cttcatgagt cagccagccc ataatcggcg cgaagataac cgttgcgaag
    15241 tattgggcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc
    15301 gcaacaatgc ggctgttgcc agggaaagat ggcgcttcag caagcccgac caggaagcgc
    15361 agtgtaaaca gggccacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc
    15421 aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacga
    15481 tccagcaacc agccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac
    15541 acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga accggcaata
    15601 gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc
    15661 cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt
    15721 gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa
    15781 atcagaggtg acgtagggtg tttttgccgt ttttataggt cgttcgccga atacggcgcg
    15841 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    15901 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    15961 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtta aaacgaagaa ataaagaaga
    16021 gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag
    16081 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacgg catttaccgc
    16141 tcgctgaaca tatcaggaca acatcgtgcc ccaatgttca acccacggat ttgattccgt
    16201 ttccggttcc gggttctcac tggcgtcaat caacagcatt tcgccaaccc gctgagcgct
    16261 ctgttcctgc aacaaggcat cgaactgttt gccgccattg cagaaattca cataactact
    16321 gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccaggg tatctttaat
    16381 tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac
    16441 caccagaaca tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac
    16501 cgttgctttg tggccctgcg cggtcagaat cgcttccgct tcttcggcca ctaacagcga
    16561 atttccgtac atagtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat
    16621 taggaactta tctctccatc ctgacccgat ggcgcactga actcaaccct ttcattttca
    16681 ggaagcagac cccgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc
    16741 gcgtgaatag ccagcggctc gccagtaaaa ggatgcgtca atgacaactg actggcatgc
    16801 agcattaacc gctgaaggcc aaaatgctca gcaccgctgc gattctggcg taaatcacca
    16861 tgtttgctgt cgccaataat cgggtgccgc aaatgggcaa gatggcgacg aagctggtgc
    16921 ttgcgtccag ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtcca
    16981 gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg
    17041 cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa
    17101 tccagcaccg cttcttccat caaccagcca cgcacaatcg catggtaacg tttctggatt
    17161 tggtgctgtt caaactgttg tgccagcagc cgtccggctt cgctggataa tcccatcaac
    17221 aacacaccag aagttggtcg gtccagacga tgagcagtaa aaacatgctg gcctatctgg
    17281 tcacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct gcggtgaacc
    17341 agccagccgg agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc
    17401 attagctcgc atcatccgca aaaagagcat ccagtttctc cagctcagcc agaataagcg
    17461 cgcgttgcgg atgatcagtc gccagcgcca tttcataata gggcgcaaca gcaaaagcgc
    17521 ccggtaacgg ctggttgtta tctaacaaat cgtgcattcg cgggatcagc acccactgca
    17581 accactccag tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg
    17641 gctggggttc atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca
    17701 actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc
    17761 gaaggatagc aggaataaaa aaagggagca ctgtattcac agtgctcccg gttcgtttcg
    17821 cagcattcca gctacttttg ttgctccctg ctcatccttg acaacttttc ctctggcctt
    17881 gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt
    17941 catcctgaag tgtccctggc cttcctgacc caccgaatca tcctgaccgg ttctcattct
    18001 ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc
    18061 tttccctgaa acaccatcct ggtgtgtcct tcagaagtgt catcatcctg atgttcactt
    18121 cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttctcgaagt cttacaaggc
    18181 gcttacagac aatgccttaa ggaaaatttc atatgaaagt taagaattaa caagcaacat
    18241 gttgttttta aatgagaata a
//
LOCUS       DACADA010000084        18225 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.085, whole genome shotgun sequence.
ACCESSION   DACADA010000084 DACADA010000000
VERSION     DACADA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.085"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            85..1305
                     /locus_tag="ISB17_002884"
     CDS             85..1305
                     /locus_tag="ISB17_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1980789.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            1278..1907
                     /locus_tag="ISB17_002885"
     CDS             1278..1907
                     /locus_tag="ISB17_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP1980790.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1908..3068)
                     /gene="ybdL"
                     /locus_tag="ISB17_002886"
     CDS             complement(1908..3068)
                     /gene="ybdL"
                     /locus_tag="ISB17_002886"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAP1980791.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTKMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3177..4265
                     /gene="hcxA"
                     /locus_tag="ISB17_002887"
     CDS             3177..4265
                     /gene="hcxA"
                     /locus_tag="ISB17_002887"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAP1980792.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4275..4472)
                     /locus_tag="ISB17_002888"
     CDS             complement(4275..4472)
                     /locus_tag="ISB17_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP1980793.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4585..6690)
                     /gene="cstA"
                     /locus_tag="ISB17_002889"
     CDS             complement(4585..6690)
                     /gene="cstA"
                     /locus_tag="ISB17_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAP1980794.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6871..7284)
                     /gene="entH"
                     /locus_tag="ISB17_002890"
     CDS             complement(6871..7284)
                     /gene="entH"
                     /locus_tag="ISB17_002890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAP1980795.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7287..8033)
                     /gene="dhbA"
                     /locus_tag="ISB17_002891"
     CDS             complement(7287..8033)
                     /gene="dhbA"
                     /locus_tag="ISB17_002891"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAP1980796.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8033..8890)
                     /gene="entB"
                     /locus_tag="ISB17_002892"
     CDS             complement(8033..8890)
                     /gene="entB"
                     /locus_tag="ISB17_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAP1980797.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8904..10514)
                     /gene="entE"
                     /locus_tag="ISB17_002893"
     CDS             complement(8904..10514)
                     /gene="entE"
                     /locus_tag="ISB17_002893"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAP1980798.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10524..11699)
                     /gene="entC"
                     /locus_tag="ISB17_002894"
     CDS             complement(10524..11699)
                     /gene="entC"
                     /locus_tag="ISB17_002894"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAP1980799.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11888..12844
                     /gene="fepB"
                     /locus_tag="ISB17_002895"
     CDS             11888..12844
                     /gene="fepB"
                     /locus_tag="ISB17_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1980800.1"
                     /translation="MRLASLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIALFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12848..14098)
                     /gene="entS"
                     /locus_tag="ISB17_002896"
     CDS             complement(12848..14098)
                     /gene="entS"
                     /locus_tag="ISB17_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAP1980801.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLSVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            14209..15213
                     /gene="fepD"
                     /locus_tag="ISB17_002897"
     CDS             14209..15213
                     /gene="fepD"
                     /locus_tag="ISB17_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAP1980802.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15210..16202
                     /gene="fepG"
                     /locus_tag="ISB17_002898"
     CDS             15210..16202
                     /gene="fepG"
                     /locus_tag="ISB17_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAP1980803.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16199..17014
                     /gene="fepC"
                     /locus_tag="ISB17_002899"
     CDS             16199..17014
                     /gene="fepC"
                     /locus_tag="ISB17_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1980804.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17011..18144)
                     /gene="fepE"
                     /locus_tag="ISB17_002900"
     CDS             complement(17011..18144)
                     /gene="fepE"
                     /locus_tag="ISB17_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAP1980805.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GQGKAIIVILSALVGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4334 a         5150 c         4657 g         4084 t
ORIGIN      
        1 atgtgctcta tcttgcttcg tttatcctat aattagcgga ttcatctcac gagtcacccc
       61 gtaacgttca gagagcgttt ttagatgtct atttataaaa tcccgcttcc actgaatatt
      121 ctggaagctg caaaagaacg tattacctgg acacttaata ccctaccccg tatctgtgtt
      181 tctttttcgg gtggtaaaga ttccggatta atgctgcatc tgacagctga aattgcccga
      241 caaatgggca aaaaaatctg cgttttgttt atcgactggg aagcgcagtt ctcatgcact
      301 attaactatg ttcagtcatt acgtgagttt tacgccgatg tcatcgaaga gttttactgg
      361 gttgcgctcc cgcttacgac gcaaaattcc ctttctcaat accaacccga atggcagtgc
      421 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
      481 tttttctcct tttaccagcc tggcatgacc ttcgaacaat ttgtgcgtga gtttgctgaa
      541 tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcaga tgagtcctac
      601 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgatga taagccctgg
      661 accaccgccg caccaggcgg tcatagctgg tacatttacc ccatctacga ctggaaagtg
      721 gctgatatct ggacctggta tgctaaccat caacagctct gtaacccact gtataatata
      781 atgtatcagg caggcgtccc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
      841 caacggcaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
      901 gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttttat
      961 ggtcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg
     1021 ctcaatagca tgccggaaaa aacagcggag cattaccgca ataaaatcgc catttatttg
     1081 cactggtatc agaaaaaaag catcacggtt ccacaaactc agcaagggga cattggggcg
     1141 aaagatatcc cctcctggcg gcgaatatgc aaagttttac tcaataacga ttactggtgt
     1201 cgggcattat catttagccc tacgaaagcg aagaactatc agcgttataa cgaacggata
     1261 aaaggaaaac gtaaggaatg ggggatacta tgcaacaacg attaacgcag gatttaaccc
     1321 aatttctcgc cagcttgccg gaagatgatc gcatcaacgc gattaatgaa atccgcatgg
     1381 cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa
     1441 acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     1501 tgctgaaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acgcatacgg
     1561 ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     1621 catcgttaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta
     1681 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgtcac caaatcactg
     1741 ccatgtcaga aatcgtccga gaactcagcc agttgggatg ggacgataat aaaattggca
     1801 aagagctggg aatggacagc gacgaagtgt tacgcctgaa gcaaattaac ggcctgcaag
     1861 agttgtttgc cgaccgtcaa ttttcccgcg cctggacggt taaataacta cagctggcgc
     1921 aggcgttctg ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     1981 ttatgtggga agggatcggc gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc
     2041 cgcgtcagcc actggcaaaa ctcaacatca tccatcgtag atacggcgct gtaatccacc
     2101 agcaaaaagt atgtaccttc gcacggtaaa atctccagcc gactttcatt taaggcattc
     2161 accagaatat cgcgcttctg gcgataaaag tccggtaatg caagataatg ctcaggttct
     2221 gcacgtagca tatcggcaag cgccagttgt gccggggtat tcaccgaaaa ggtcagatac
     2281 tgatgcactt tgcggatctc ggcgctgatg ggcgcaggcg caacgcaata acccactttc
     2341 cagccggtca tatgataggt cttaccaaac gatgacaccg ccaccgctcg ctcacgcaat
     2401 tgcggatgcg ccagcacgct ggcatggccc tgttgtgaaa aattgatgtg ctcgtagact
     2461 tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacagagc ggcgaaatca
     2521 gtctgctgcc agacagttgc actggggtta tgcggggtgt tgaggatcac cagtcgggtg
     2581 cgctcgctta acaatgcggc aaattcctgc cagtcaacgc gaaaatgcgg cggttgcagt
     2641 gcaatacgtt tcactattcc ccccgaaagt gcgatggcgg gggcgtaact gtcatagctg
     2701 ggatcaaaac aaatcacttc gtcgccattg cgcaccaatg cggttatcgc cgcgtataac
     2761 gcctccgtcg cccctgccgt cacggtgata ttgctgtcga catccggttg atagccatac
     2821 agacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggtgcg
     2881 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     2941 tcaaaatcag gaaagccttg cgaaaggtta atcgcctggt gttgctgcgc cagcgcgctc
     3001 atcttggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     3061 tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta
     3121 gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc
     3181 ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat cccggaagtt
     3241 tcaatcacct gcacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg
     3301 gcgaacgcgc cattgctgcg gcacaaacca aactgccgcc tgcgtttgaa ctgccagggg
     3361 taaagcatat tttgtttcgt ggtcattgca gcgaaagcga cgtgcaacaa ctggcggcgg
     3421 agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcacta ctcgacaccg
     3481 cgaaagccct cgcccgccgt ctcggtctgc cgtttgttgc cgttccgacg atcgccgcta
     3541 cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacaa gcgctgcatt
     3601 atgagatttt cgacgacgcc aattttatgg tgctggtgga accggagatt atcctcaatg
     3661 cgccacaaga atatctactg gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg
     3721 tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcggctggga atcaataatg
     3781 cgcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcgcttgcc gatcagcaaa
     3841 atcagcaatt aacgcaatca ttttgcgatg tggtggatgc gattattgct ggtggtggga
     3901 tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggcccatgcc gtgcataacg
     3961 gtctgaccgt tttgccgcaa accgagaagt ttctccacgg caccaaagtc gcctacggaa
     4021 ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta accggggcgt
     4081 atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc aataatcagg
     4141 tggagatcga caaaatgatt gcccacaccc tacgtccggt ggaatccatt cattacctgc
     4201 cagtcacgct gacaccagat accttgcgtg cagcgttcga aaaagtggaa tcgtttaaag
     4261 cctgacgtac aaatttagca gcagcgcgcg ccgccttttc caccatagcg cgcgtcctgc
     4321 cgctcacgga aaaactcctc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata
     4381 tgttcgacat agttgtcgta atcaggcata ccaatcatca gtttcgccgc ctgacctaaa
     4441 tattttccgg cttttgccag tgaatcaaac atctaaacct ccggtaatac aaatgccctc
     4501 ctgaattaag agggcttaat tgctatcaac acgctcgggc tgagagtaag gggaacgttc
     4561 agcacatccc ccctcactct gactttagtg cgcgcctttc gcctgcgcca cgatctcctc
     4621 gacattttcc ggcatcggct catatggcgt ttctttcgcc gttggcttcg gctctttcaa
     4681 tgccgccagt gccgtcttaa tcgagaacag cgccagaacc accacgacca ccataaagaa
     4741 gatggttaac ccggcatcaa gacggttgtt gaacaccagt tgcgccagtt gtgactcagt
     4801 atactgcgat ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatcgccag
     4861 gaatccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca gggtacaaat
     4921 cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcattttgaa
     4981 cagcaccaca gcacaaagca tcagcgccat ccctgccagc atctggttgg caataccaaa
     5041 cagcggccac aaggtgttaa taccgcccag cggatcgacc tcgccctgat gcaggaagta
     5101 gccccacgcc agcacgcaca gcgccgttgc cagcaggtta gcaggcagtg aatcggtccg
     5161 tttcaggccc ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt
     5221 acccgcatcc accgccgtca gaataaacag tgcttcaaac agaatggcga agtgatacca
     5281 gaacgccaca tccatcatgc cgcccagcgc accgtgcaga atgtaggcca tccccaccgc
     5341 cagggtcggc gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat
     5401 ctggtttagc gtatccgggg taatcgcaaa gccccagcta ctcaccacct gcgcggcaga
     5461 agcaaccaca tccgccgtcc ctgccggagc cagcaccgcc atcgggctgt tcatggcgaa
     5521 gtacacaccc ggatcgatga tacaggcgga aaccagtgcc ataatcgcca caaaggattc
     5581 cattaacatc ccgccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg
     5641 cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcgccacagg cgatggtgat
     5701 aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttagt
     5761 tagcgcaggc atggtcagcg tcgggcgcat aattaaaatg cccaccgcca gaccgacgat
     5821 agtcccgatt ttcaggaagg ttgagaggta gtcacgcgga gccagcagca accacaccgg
     5881 cagcaccgcc gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa
     5941 gtcaaagtac ggtgcccagg tcgggctttc tgccacccag ccgccagaga taatggcgaa
     6001 aatcaggaac accagaccaa ttaccgacac ttcgccaata cgccccggac gcaaatagcg
     6061 caggtagatc cccataaaca gcgccagcgg aatggtgaac gcaacggtat acgttcccca
     6121 cgggctatga gtcagggctt tcaccacaat catcgccagc acggcaagga taatgaccat
     6181 gatcataaag caggccacca gcgctatcac cccggcggtt ggtcccatct cttctttgac
     6241 cagctcaccc agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg
     6301 caccgcaccg gcgagaacca ccccggcgag cagccagatc atccctggca ggtaacccat
     6361 ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg caaaatggtg
     6421 accgaacagc acttttttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc
     6481 tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca ggccataaaa
     6541 acggtaggcg attagataga tacagaccga cgccaccaca atccacagcg cgttgatctg
     6601 ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag cgagcactgt
     6661 ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga
     6721 tgttacattt tgtttctata acatctttgt acaatttaac aacacagcaa ccatgtaaat
     6781 cggaattgaa accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt
     6841 ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca
     6901 ggtgcagcag cgccgcccct gttcatcgaa aacgacaatt tcccagctct gattttgtcg
     6961 tccaagatgc agcggctggc agacaccgcg cacctttccc tcagacaccg ggcgatgatg
     7021 tgtggcgtta agttccgtgc ccaccacaca ctgtccgtcg cgggtcatca taaatccggc
     7081 catcgatccc agcgtttccg ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg
     7141 ctgatgagta cgggtatcaa ccggcatttc ggcttcgagt acatcatcgc ccagacgggt
     7201 atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca gctcgtcgag
     7261 cgttaaatga cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca
     7321 atatcctgta gggtaatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg
     7381 atctcttgtg gacaggcgat tttccctagc ggaatgccga gtttaaactg ctcgccaaag
     7441 ccgcgaatac gctgctcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg
     7501 tcggtggagc ccggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg
     7561 acgctcaacg ccaggctttt cagcgccgct ttcgacgcgc cataagcact cataccaata
     7621 cgcggcgtgt gtgcggcgtc ggacgcaaca gtaacaatcg ccccgccccg ctgacggcga
     7681 aactggttca tggtctgctg aaacaggtta aacgcaccgc cgacgttgac cgcaaaagtc
     7741 tgctgccagt cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca
     7801 ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc
     7861 tgcgcagcat cggcaacatc cattacttcg gtcgcaaaag gatattgctc ctgactaaac
     7921 gcttgatcaa aacctgtaac tttcgccccc gcctcaacaa atgccagcgc cgtggcgtag
     7981 cctatacctt tccccgcgcc agttacccag acatttttac cgctgaaatc cattatttca
     8041 cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa
     8101 agtcgatatc gccatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat
     8161 ccagaccgta gtcgatcagg ttgtcgtcat cgaagggttc atcggactcg tccagcaacg
     8221 gcaggatcac ctcacgcagc gccgctttgc tggcggggat aggtgctggc agcaattctt
     8281 cggtcatcac cacccgacca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt
     8341 cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg
     8401 cgtccgttgc ggtggtcata cagccaatgt gggcatatac cccggtaata atcagctggt
     8461 tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaatgcg ctgtagcgcc
     8521 atttcaccag caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt
     8581 ccggcgaacg ggtcaggcca ggcccccaca tatcattcaa cagcgcccga tcttcatcgc
     8641 tctgctcttt cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc
     8701 gtagtgccgc aatattcgcg atcacctgct ccatcatcgg gcagttctca ccccagaagc
     8761 tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc
     8821 agtcgacttt attctgcgga atatcgtgag gctccggcag tgcgtaagcc tgtaattttg
     8881 gaatagccat cgtgttctct ccttcaggct gatgtgcgtg acgccagcca ctgacgtaat
     8941 tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca ctccacgcga
     9001 tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg
     9061 cgcagcggct cttttaccac cagataagca cagctttttt cgcccatcag ctcatcttcc
     9121 atgctcacca gtgcggcgta gatcaccgcc ggatggcgca gcagcaggtt ttcgatctct
     9181 tcggcagcga tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacagtg
     9241 atgtaaccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca
     9301 aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaagtgta cggcccgcgc
     9361 gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccat
     9421 acttcatcgt ccgggcacat tgggtaaccc tgggtatgga taattttctc cgcactatca
     9481 tcaagacggg tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag
     9541 ccaatctcag cgggaatacg cgccgcaagc gtggcagaaa gacgtgcgcc gccgacctgt
     9601 aacagtttca gcgaggcaag ctgcgcccgg ctttcgcctt cagccagtgc ctgcaaccac
     9661 aggctgactg ctggcggcac cagcgcggtg acgttcacct gatgtttttc aatcaatggg
     9721 aagcaaagcg tggcgctggg gtcggcagcc agaacgacag tgccgccagc gagaaatacc
     9781 cccaacgatc ccggtgaact catggcgtag ttatgagccg ccgggatcgc gcacaggtag
     9841 cgcgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta gtagtagtcg
     9901 ttatgagtgc gcgggatcag tttcggagtg ccggtggtgc cgccggaaag ctggaaatag
     9961 gccacttcat cagcaggaga tggcgtggca gtaaaatcct cagccggatg gttaatcgca
    10021 tcctgcaagt tatgctcacc gctgtcgttg agcagctgca ccacgcgaat ggaagaatgc
    10081 tctgcaacaa atgtgttgag gaaatcatcc ccgctaaata gcgcatgttg gcgatcggca
    10141 atcagtaacg ccggttcaat ctggctggca taggcgttca gttcactacg ctgatggctg
    10201 aacagcgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt
    10261 tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc
    10321 aaactacagg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa ctgccactcg
    10381 ccgtcgatca ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc
    10441 aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg ccatcgggtg
    10501 aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt
    10561 ttgacgcctg tttcgcgcca ctcacccaac ggtgaggacg caggcacaat ccccgctccg
    10621 gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattca
    10681 ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc
    10741 tccagttcag caataacctg agtcgcggcc agatgcggga aaccgctcag cgcaggagtt
    10801 ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag
    10861 ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt
    10921 aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag ttcatgttca
    10981 tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatct
    11041 ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt
    11101 agcagcagtt ccgggctggc ccccagcaga acgccaccat cagccaacgg aacatggaag
    11161 ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg
    11221 gcgtcagtgg tgatatcaat caaccgtgac aacaccactt tgtcgacctg cggcgtggcg
    11281 gtaagtgcgg cagcacgggc aaccatctgt tcaaacgtgg tttgttccgg aatggcctga
    11341 cgttccacca cgttcagcga ctggctgcgg gtgaaacggc gtgcggaagc ttgtttttcc
    11401 tgacgggaga acgactgcca ggattcggga atatacagcg acgaaggctg acgtggatcg
    11461 aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac
    11521 agcgcggcga gtttttgctg gaagggactg tcgggtgaat ccccgttcac agcgggttca
    11581 tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag
    11641 cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata
    11701 tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt ttcggtcgct
    11761 aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg
    11821 ttgcatctgg ttgtggttac aatgaaaatg agaagcatta acttcattaa taacaggaag
    11881 ctgatttgtg agactcgcct cgctctaccg caacgccctt ctattaacag gacttttgct
    11941 ttcaggaata gccgcagttc aggccgccga ctggccgcgt cagattactg acagccgtgg
    12001 cactcatacc ctggaaagcc agccgcagcg tattgtatcc accagcgtca ccctgactgg
    12061 ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacac caaataaccg
    12121 cgtagcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag aacgcaaact
    12181 gcaacggctc tatatcggcg aaccgagtgc agaagccgtt gccgcgcaaa tgccggatct
    12241 gattttaatt agcgcaaccg gcggcgattc ggcgctggcg ctgtacgatc agctttctac
    12301 catcgcccca acattaatca tcaattacga cgacaaaagc tggcagtcgc tgttaacgca
    12361 gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac tgtttgataa
    12421 gcaactggca gcggcgaaag agcaaatcaa attaccgccg cagccggtca ctgcccttgt
    12481 ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac aagggcagat
    12541 gctggaacaa ctcggcttta cgctggcgaa gttgcccgca ggcttaaacg ccagccaaag
    12601 ccagggcaaa cgccatgaca tcattcagct tggtggagaa aatctggctg cagggttaaa
    12661 tggcgagtca ctattcctgt tcgccggtga tcagaaagac gccgatgcta tttatgctaa
    12721 tccgctgctc gcgcacctgc ctgcagtaca aaacaagcag gtttatgcgc tgggaaccga
    12781 gacattccgc ctggattact acagcgccat gcaagtgctg gaacgactta aggcgctgtt
    12841 ttaagcatta attgtcggac gctgtcacct gcggcggcgt ctggcgaaaa cgccgcaact
    12901 ccaccagcac cagcaacaac aacacgccga taatcaacaa accaaaaccg ctcgcgcttg
    12961 cggaagcaac cggtgtcatc atcgctccca gaccgcctaa cagcgccgca cctatggcat
    13021 cgcccgtcac gttttgcgcc gtccacaaac cgttaatccg ccctaacatc gcttccgggg
    13081 tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc cagccgaaca
    13141 gtgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga ccaatggcga
    13201 ggaacgcccc caatgtggag agcaacatca acagacctgg ccgtacgcta tgcgccagtt
    13261 ttccactggt taacgcgcca atagccgcac cgagcgggat tgccgcatag agaaagccaa
    13321 tttgcgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc cgcaccgcgc
    13381 tcgccatcgt caataaaccc cccagcaacg caatccctcc caccagcggg ctggcgagta
    13441 gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc ggcggtggtg
    13501 gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc gccgccagcc
    13561 cgtagttcca ggctacgcct ccggtcgcca gcagtaaacc accaatcatg ggcgaaatca
    13621 ccgaccccag acgcacagtc aacatggtga tcgccccggc ctgcattagg ttttcacgtc
    13681 ctaccagtgc aggtgtcgcc gccagtagcg ccgtaacgcc aagtgatgcg aaaaaaccat
    13741 cccataaacc aagtaaatag attgccagca aagacggctc cggcagcagc gcattcaggc
    13801 acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact tttttgcgct
    13861 catagcgatc cgccagcaca ccaccgatca tcagaccgac aaacatagca ccgccggtca
    13921 gcgtcaccga aagccccacc tgccaggtag aatgtgtcat catctggatc tgcaccggca
    13981 ccgcgacgct gagcaaaccc agagacacaa ttgagataaa acgagcgagg aatactgcgc
    14041 gaaacgccgg gtgcgttttt aacaggctga ggttaagcag ccaggattgt ttattcatta
    14101 caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg ctaacatatc
    14161 caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat gtctggttct
    14221 gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat tatcgcgacg
    14281 gcattaagcc tgctcattgg agcaaaatca ctccccgctt ccgtagtgct ggaagccctc
    14341 tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcacgact gccgcgcacc
    14401 cttgccggtt tactggcagg cggtgcgctt ggccttgccg gggcgttaat gcaaaccctc
    14461 acccgaaacc cacttgccga ccccggcttg cttggcgtga atgccggagc cagctttgcc
    14521 attgtgctgg gtgcggcgct gtttggttac tcttccgcgc aggaacaact gatgatggcc
    14581 ttcgccggcg cgctggtggc gtcattgatt gttgccttta ccggcagcca gggcggcggg
    14641 cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt gctggaagga
    14701 ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg tttctggcaa
    14761 gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat cccggtgctt
    14821 accgccggag caaccgcgct attactgagc cgcgcgctga acagtttaag ccttggcagt
    14881 gacaccgcga cggcgctggg cagtcgcgta gcgcgcacac agttgattgg tctgctggca
    14941 attaccgtgc tttgtggtag tgcaacggcg gtagttggcc cgattgcctt tattggcctg
    15001 atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc gctgcccgtc
    15061 acgctactcg ctacccctgc cctgctgctg tttgccgata tcattgggcg ggtgattgtt
    15121 cccggcgaac tgcgcgtttc tgtagtcagt gcctttattg gtgcaccagt gctgatcttc
    15181 ctcgtacgac gtaaaacgcg aggtggcgca tgatttacgt ctctcgccga ttaattatca
    15241 cctgtctact gttgctaata gcttgtgtaa tggcaggcgt gtggggatta cgcagcggtg
    15301 ccgtcacgct ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg ccgcgcagta
    15361 tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg ttgattggcg
    15421 cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg ctcggcagcc
    15481 ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg atggtgctgt
    15541 ttggtcagga tctgacggct atcgcgctgg cagcaatggc ggggggtatt gtcacctcgc
    15601 tgctggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg attattatcg
    15661 gtatcggcgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg aaagcgtctt
    15721 tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc ctgacgtggg
    15781 caaaaacctc accttccgca cccatcatta tattaatgtt aattgccgcc gccttgctgg
    15841 tgcggcgaat gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg ggcgtcagcg
    15901 tcgaacgttc gcggctgtta atgatgctgg tcgccgtagt gcttaccgca gctgccactg
    15961 ccctggcggg gccaatttcc tttattgctt tagtcgcgcc acacattgcc cgccgcatca
    16021 gtggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggggcactt ttactgctgg
    16081 cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt ggcgtcgtta
    16141 ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct cgcaaaaaat
    16201 gaccgaatca gtagcccgtt tgcgtggcga acagttaacc ctcggatatg gcaaatatac
    16261 cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa ttatcgggcc
    16321 aaatggctgc ggtaaatcca cgttactgcg taccttaagc cgcctgatga cgcctgctca
    16381 cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag aggttgcacg
    16441 ccggattggt ctgttggcgc aaaatgctac cacaccgggc gatatcaccg tgcaggagct
    16501 ggtggcgcgt gggcgttacc cgcatcaacc gctgtttacc cgctggcgca aagaagatga
    16561 agacgcggta acaaaagcga tgcaggccac gggaataact catctggcgg atcaaagcgt
    16621 ggacaccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc tggcccagga
    16681 aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc atcagattga
    16741 tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg cggcggtgct
    16801 gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc gggaagggaa
    16861 aattgttgct cagggagcgc cgaaagagat tgtcactgct gaactgatcg agcgcattta
    16921 tggtctgcgc tgcatgataa ttgacgatcc ggttgccgga acgccgctcg tggtgccgct
    16981 cggacgaacg gcaccttcaa ccgcaaagat ttaaactaag tggtctgcca tcatggcatc
    17041 ctgttttctg gatgccatcg catgacgcaa caacacacca ccacaagcca ccatcccgcc
    17101 gaccaacgca gaaaggatca cgataatcgc cttaccctga ccgtcttttt ttacgggtaa
    17161 tgacggacgc aactgatatt taaacggtgt aaaattcaca tcgttgacat ttgtttttgt
    17221 taattgctcg actagatact gacgattacg taattcaccg ttcaattccg caacgtcagt
    17281 gactgctttt tcaatttcca gtttgcgttc aataccgtct gcaccgagag aaatagagaa
    17341 atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa ttcctgccgc
    17401 gttggcaatg tcaagtgaat aattgaggcg ctgaatgttt gcatcaagtt gatttttcgt
    17461 tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct ccagtttatt
    17521 acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga tatacccggc
    17581 caacactttc tgtgcctctt cactggtggg tgcggtgaaa cttagcgtcc aggaagtata
    17641 caatgcagac tcatcttttt tcttactggc attgtcatca accgctttca ttttttcgct
    17701 caatgcaaca atggcgcgat gcagatccag tgggtcgatt ttcgcttctt ttaattgatc
    17761 catcacataa ggtgatgaac gcaggtactc ctcaagcaag ctaacagact gaaatttctt
    17821 gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca gcacacgaag
    17881 cttagtgaat gttttctcca gctcttgcca ctgaacaggt tctggaggcg tgacaaccgc
    17941 cgcgctagtc catttttgcg gcaggatgaa agagatcagc aagcctgcgc aggcaaacgc
    18001 aaaaacgacc gccatgaccg ttttttttgc ccgccataaa acctcgatta gattaagtaa
    18061 atcaatttca ttattactgg gcgacgccag aggatactcg ggaaaatgag cttcacttcc
    18121 ctgtttaata ttcagtgatg acatgtagtg aaacctgagg ttttagtccg taaagcacaa
    18181 cgatgtacat agcattctaa caggaactga gatttatccg aaagc
//
LOCUS       DACADA010000085        18166 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.086, whole genome shotgun sequence.
ACCESSION   DACADA010000085 DACADA010000000
VERSION     DACADA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.086"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(213..959)
                     /gene="cutC"
                     /locus_tag="ISB17_002901"
     CDS             complement(213..959)
                     /gene="cutC"
                     /locus_tag="ISB17_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP1980806.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(973..1539)
                     /locus_tag="ISB17_002902"
     CDS             complement(973..1539)
                     /locus_tag="ISB17_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1980807.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1755..3488
                     /gene="argS"
                     /locus_tag="ISB17_002903"
     CDS             1755..3488
                     /gene="argS"
                     /locus_tag="ISB17_002903"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP1980808.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3541..3933)
                     /locus_tag="ISB17_002904"
     CDS             complement(3541..3933)
                     /locus_tag="ISB17_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAP1980809.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(3933..6011)
                     /gene="flhA"
                     /locus_tag="ISB17_002905"
     CDS             complement(3933..6011)
                     /gene="flhA"
                     /locus_tag="ISB17_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP1980810.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6004..7152)
                     /gene="flhB"
                     /locus_tag="ISB17_002906"
     CDS             complement(6004..7152)
                     /gene="flhB"
                     /locus_tag="ISB17_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP1980811.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7341..7985)
                     /gene="cheZ"
                     /locus_tag="ISB17_002907"
     CDS             complement(7341..7985)
                     /gene="cheZ"
                     /locus_tag="ISB17_002907"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAP1980812.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVIDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQGQVDDLLDSLGF"
     gene            complement(7996..8385)
                     /gene="cheY"
                     /locus_tag="ISB17_002908"
     CDS             complement(7996..8385)
                     /gene="cheY"
                     /locus_tag="ISB17_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAP1980813.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8400..9449)
                     /locus_tag="ISB17_002909"
     CDS             complement(8400..9449)
                     /locus_tag="ISB17_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAP1980814.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9452..10312)
                     /gene="cheR"
                     /locus_tag="ISB17_002910"
     CDS             complement(9452..10312)
                     /gene="cheR"
                     /locus_tag="ISB17_002910"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAP1980815.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10331..10557)
                     /locus_tag="ISB17_002911"
                     /pseudo
     CDS             complement(10331..10557)
                     /locus_tag="ISB17_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10603..12264)
                     /gene="tar"
                     /locus_tag="ISB17_002912"
     CDS             complement(10603..12264)
                     /gene="tar"
                     /locus_tag="ISB17_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAP1980816.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQSELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLAAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLAATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12409..12912)
                     /gene="cheW"
                     /locus_tag="ISB17_002913"
     CDS             complement(12409..12912)
                     /gene="cheW"
                     /locus_tag="ISB17_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAP1980817.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12933..14897)
                     /gene="cheA"
                     /locus_tag="ISB17_002914"
     CDS             complement(12933..14897)
                     /gene="cheA"
                     /locus_tag="ISB17_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAP1980818.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTSPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14902..15828)
                     /gene="motB"
                     /locus_tag="ISB17_002915"
     CDS             complement(14902..15828)
                     /gene="motB"
                     /locus_tag="ISB17_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAP1980819.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15825..16712)
                     /gene="motA"
                     /locus_tag="ISB17_002916"
     CDS             complement(15825..16712)
                     /gene="motA"
                     /locus_tag="ISB17_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1980820.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16839..17417)
                     /gene="flhC"
                     /locus_tag="ISB17_002917"
     CDS             complement(16839..17417)
                     /gene="flhC"
                     /locus_tag="ISB17_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAP1980821.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17420..17770)
                     /gene="flhD"
                     /locus_tag="ISB17_002918"
     CDS             complement(17420..17770)
                     /gene="flhD"
                     /locus_tag="ISB17_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAP1980822.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4358 a         5020 c         4453 g         4335 t
ORIGIN      
        1 tatttgtcag ttttggcaat ttaaatcaca taatgaatgt gaatatatag aatatttcta
       61 gcacaatgct atgcgcactg tcacccgctc aacagatcac ggcaatcgtt cgttttttat
      121 actactcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca
      181 tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt
      241 cctttcattt cagcaaccgc cgccccgtct acgacataac gcgaatactc gtccgcgtgt
      301 gcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct
      361 cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag gttttccgca
      421 cggactcctg ctccggccat aatgattgga gcatcacgat gggcaataag ttccataatt
      481 tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc
      541 aattccgtaa gattattgag tgtatttaaa gggttagcgc acatatcgaa ggcgcgatga
      601 aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca
      661 ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggactgtg
      721 cgcacatctt caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt
      781 gggcggataa tcggatgcac tgggatcgtc acccgctggc gcacggattt cagtaccccc
      841 aacgacggcg ttaggccccc ctcttttgga gctgcgcata attcaactct gtcagcgccg
      901 ttttgctgcg ccgttagcgc gcattccatg ctgtaacagc aaatttccag taatgccatt
      961 tttgctcctt agttacgccg actgctcgct ggcaacgatc tcttcaatgg accacggatg
     1021 aaacttaatg gttgttttac catcggtgac cgccagcgtc ggattcggta atcgttcatg
     1081 ctcacctttt ggcgaactgg ttttaactga gatccccggc aggctgagtc cttcatcaga
     1141 aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggtagaa cgatttcaat
     1201 atgttcccaa ccttcgtgcg ggtaacgttt ttccccgggc cacggtagct ccacaatgga
     1261 aaactgccag tgcgcaacct gtagcggttc atgcaattta aacaaacaaa tcggtctgcc
     1321 attgatcata ttttctgaca aaagctcgcc gcactgttca aacccgcgac gccagcgttc
     1381 agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc
     1441 caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc
     1501 aatatcctgc agctcgtcga tagattgcca gttcgccata atcattcttc gtctttcatt
     1561 aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc gctgatttca cgccgcttct
     1621 gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg
     1681 cagtatactc ccgccctaat ttctttaact ggtgcgggta atttttgctc gcttcatcaa
     1741 tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga
     1801 ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg tcagtcagca aaagttcagt
     1861 tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc
     1921 gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg
     1981 agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg
     2041 ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg
     2101 tggttgacta ctctgcgcca aacgtggcga aagagatgca tgtcggtcac ctgcgctcta
     2161 ccattattgg tgacgcagca gttcgtactc tggagttcct cggtcacaaa gtgattcgcg
     2221 caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc
     2281 agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg
     2341 cgaaaaagca ttacgacgag gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa
     2401 aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca
     2461 tgacgcagaa ccagatcacc tacgatcgtc tgaacgtgac gttgacccgc gatgacgtca
     2521 tgggtgaaag tctctacaac ccaatgctgc cgggtatcgt ggcggacctg aaagccaaag
     2581 gtctggcagt agaaagtgaa ggcgcgaccg ttgtattcct tgatgagttc aaaaacaagg
     2641 aaggcgaacc gatgggcgtt atcattcaga agaaagatgg cggctatctc tacaccacca
     2701 ctgatatcgc ctgcgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt
     2761 acatcgattc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcgg
     2821 gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggcaaag
     2881 atggtaaacc gttcaaaacc cgcgctggcg gtacggtgaa actggccgat ctgctggatg
     2941 aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagccgacg
     3001 agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca
     3061 aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata
     3121 ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa
     3181 ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac
     3241 tggcagctcg cctgctgcag tttgaagaaa ccctcactgt ggttgcccgt gaaggcacgc
     3301 cgcatgtgat gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctatgagc
     3361 actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac
     3421 aactgacggc gaagacgctg aagctgggtc tggatacgct gggtattgag acagtagagc
     3481 gtatgtaatc gcgttatacg gcaacagccg atgtcattgc agacatcggc ttttttatca
     3541 ttaacgataa tttacaatca cttcattccg ttgaatcttt agcggcggag ttagccgccc
     3601 accgccaggc acttcccaga taaagcgcaa cggttctgcc gccggtatac cggaaaaggc
     3661 catggtggtt ccactctgcc cctccaattc gacacaacga gattgtgaac acaagcgaac
     3721 ccgcagtcct gcaggtgtcg gaccgataag ctgataacgc cacgccacca gcgtcatcaa
     3781 tcctgaagca ggttgtcgcg tagaaagagg cgctgacgac atcgattcac cgcgatgatt
     3841 tagcgtaacg cccacgctac tcgcctgcca catcccctcc ccggcggctt gcaccagcag
     3901 cggaaacaat aatattgcta ataaggctct cattatttgc cgccaatcgt cgccgtcata
     3961 cggatatgtc ggttatctga tagttccaga ttcgacagca ccactaactg cggcaagctg
     4021 cggcgcagga agcgagacaa taatggtcgt agcgcgtggt tcaccaacaa taccggcggc
     4081 gcacccaaca tctcctgacg ggatagcgct tcctgagttt gcgccagtaa acgatccgcc
     4141 agccctggct ccagtccacc accgccttgc aacgcctgta gcaacaaacg ttccagcggt
     4201 gtatcgaggc caataacatg gacttcatct ttgccaggaa accactgctg ggtaatcgcc
     4261 cgtcccaacg ctacgcgcac gacagcggtt aattcatgcg gatcgctttg gatgggcgca
     4321 tgttccgcca gcgtttcgag aatggtgcgc atatcgcgaa tcggcacttt ttcatcgagc
     4381 agattttgca gcactttatg cagtgtggta agcgtgacga cgccaggaac gagatcttcc
     4441 gtcagctttg gcatctcctg ggcgacgcga tccagcagct gttgtgcctc ctgacgacca
     4501 aacagctctg cggcatgctg gctaatgagg tggttaagat gcgttgccac caccgtgctg
     4561 gcctcaacca cggtgtaccc ctgaatctgc gcctgttctt taagcgcact ttcaatccag
     4621 atagcattca ggccaaatgc cggatcaacg gtcgcttcac caggtaatgt tccggcggca
     4681 gtgcctggat taattgccag ccagcgcccc ggataagcat caccactccc catttcgaca
     4741 cctttcatca agatacgata gcgggctggt tgcaaatcca tgttatcgcg gatgtgcacc
     4801 actgggggta ggaagcccat ctcctgggca aatttcttgc ggatactgcg tatacggccc
     4861 aacaactcac catcctgctg aaaatcaacc atcgggatca accgataccc cacttccatt
     4921 cccagagaat cttccagttg tacatcgttc cacgttgctt cgactaccga attattctct
     4981 gccattttta ccggttttgg ttcggcaggc gctttttgtt cacgtccgcg gatccaccag
     5041 gccagcccca gcaatccggc agtgaacagc aaaaatacca ggtttggcat tccaggcacc
     5101 aggccgagta aaccgagcac ggcggcgctt aataacataa cgcttgggtt actgaaaagc
     5161 tgattcacca tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgaccccc
     5221 gcggcggtag aaatcaccaa cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat
     5281 agcgtataac tttccgccgc gtgccccatg ctcatgccat gttgcagcac accgaccagc
     5341 aatccgccga caacgttaat gaccatgatg aggatcccgg cgatggcatc gccgcgaaca
     5401 aacttacttg ccccgtccat cgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     5461 cgttttttcg cttcatcttc accaatcaat ccggcgttaa ggtcggcgtc aatcgccatc
     5521 tgcttacccg gcataccatc gagaacaaag cgcgcgccca cttccgcaat acgcccggca
     5581 cctttggtga taaccataaa gttgatgatc acgagaatga cgaacaccac gataccgata
     5641 gcgaaattgc caccgacgag gaagtgaccg aacgcttcga ccacctttcc agccgccgcc
     5701 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     5761 aacagcgtgg taaacaacag aatggtcgga aacgcagcaa actcgagcgt gcgctgggta
     5821 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa cagcaggtcg
     5881 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     5941 ccggcaagga tctgccattg tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     6001 agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg
     6061 agtaggttgt acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca
     6121 ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac cgatctccgc
     6181 atgtcgatac agcgcacgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc
     6241 gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc
     6301 gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat
     6361 gacatccgct ttcggcacat cggccatcat ccgacgccgt gcggccgctc gctgcatctg
     6421 acggatccgc cctttaacat gggggtcgcc ttcgctttgt ttgaactcat cacgaatatc
     6481 ctgccgtgac attcgcagct ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc
     6541 caccattgga atgacaccaa gcaccaccag cagcgcgcat agccctacca gatccattgc
     6601 attacccatg gcggtaatcg gagactcggc cattaagcgc atcatctgcg gccagtgata
     6661 ccagagaaaa aaccccgtca cgctgccaac caggatggtt ttcaaaattg ccttaagcaa
     6721 ctccgcgcca gtctgtgccg agaacgtccg tttaatgccc ggtagcgggt tgagtttgga
     6781 aaacttcggc tgcaaggatt ttccgctaaa taccagcccc cccagcataa ccggagaaat
     6841 aatcgccacc agcaccacgc cgctaatcag cggtagcagc gccagcatgg cttctctgat
     6901 cagcagaata atctgcccga ggatcagatt cgggtcattg ataatactgt gatcaaaatg
     6961 tagccctgcg gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat
     7021 aacactaacg cccaccagca aaatcagcag tgaagtcagt tcacgggaac gcgggatttg
     7081 cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg gcctctgttt tgtcgtcgct
     7141 ctcgtcagac acgtcgccaa tcctggaaag agtattaagc gtaaatgatg ccagagcgca
     7201 aagcgttcaa tggtttgagt aaggggcaaa acaggcggga tttagggctt ttactgccac
     7261 acatcaagca tagtatgcgt ttgtcggatg cggcgtcatc gcctgatatg acgtggtcac
     7321 gtcacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc cacctgaccc
     7381 tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag caaactctgg
     7441 ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacaacac catcagcaac
     7501 tgacgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc ggtgagatcc
     7561 tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct ggtatgcgcg
     7621 gggacatctg ccagaaattg ccgtgtatcg atcaccagtt cacgggcgtc ggcaaggtca
     7681 atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga tttctccatt
     7741 tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc agcctgggcg
     7801 gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc cgctgcttcg
     7861 gcaatggcct gatccagccc cagttcccgc aaactgtcgc gcagcatacg cgtcaggctg
     7921 ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat tgatggttgc
     7981 atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt tttcctccag
     8041 cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg ccgcagcaat
     8101 gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg acatcgcgcc
     8161 atccgcacga atcgttttca gcaactccag gccatccata ttgggcatgt tccagtcgga
     8221 gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc catcttccgc
     8281 ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc gtcgcatggt
     8341 ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac actcctgatt
     8401 taaatacgta tcgcctgtcc ggcactaatt tttgccagca tttgctgact tacctggcta
     8461 agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat gccgaacacc
     8521 acgcaacttg cttcgttttg cgcaagggtc catgcccccg cctgacgcat cgccaacatt
     8581 cccgccgcgc cgtcgttgcc cataccggtt aggattaccc caaccgcatt acgccccgcc
     8641 tgtttggcca cagaatggaa caacacatct accgatggcc gatggcgatt aaccgccggg
     8701 ccatcgtgaa ttttgatttg ataatttgcg ccactacgcg ccagctccat atgccgatcg
     8761 cccggcgcga tataggcatg tcccggcaag acgcgctctc catcttcggc ttctttaacc
     8821 ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc gggcggcata
     8881 tgctgggtaa ttaacagtgc cggactggaa agcggcaacg gttgcagtac gtgacgaatt
     8941 gcctcagttc cccccgttga agcaccaatc gcaatcagtt tttcagaact caacaacggc
     9001 cccgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct cgcctttgct
     9061 gccgtacgca ccttttcagc aatcatttcg ctatacgcca gcattccttc gcgaataccc
     9121 agttgcggtt tggtgacaaa atctatcgcc cccaactcca gcgcgcgcag cgtgacttct
     9181 gaccctttgc cggtcaggga agaaaccatc acaacgggca ttggacgcaa acgcattaat
     9241 ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt caatacatcg
     9301 ggattgaatt tcttaatcaa atcacgcgca accagcggat caggggcggt cgccaccatt
     9361 tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag tgccgaatca
     9421 tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca tacaccgtct
     9481 gaccacgcag cgtgaagcgg cgctcaaggt ggctaaagtt ttcagagtga cccgcaaaca
     9541 gcaatccgtc gggtttaagg agcgaaacaa agcggcgcaa aatctcctgc tgggtagttt
     9601 gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc ggcacggtgt
     9661 actgtttcgc cagcaaattc agcggggcaa aatcaacata gttcgccagc tcctgacgca
     9721 cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc agttgctgcg
     9781 gcgtcaggtt tttcaactct tcatggcgat aaataccgct tctggctttt tccagcactt
     9841 cggtgtcgat atcgctggca aacactttcc agcgtccggg cgcggtgccc aatgtatcag
     9901 ccagcgtcat cgctatgctg tacggctctt cgccggttga agccgccgcg ctccatacgc
     9961 gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag aggaaaatgg tgtgcctcac
    10021 ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac tcaccgctgt
    10081 gctgattaga ttccagcaag ttcaggtaat gaccgaaatc cgccagtccc agcgcacgca
    10141 aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga acgatcccgg
    10201 cgcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc gccaggcgct
    10261 cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc atagcgcctt
    10321 ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca tgtcgtgcta
    10381 cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg gcctgattca
    10441 gtcgttgcaa atcgtcgcga aatgcccaaa agacatgccg ttactgccaa tctgcaagat
    10501 cccgcagaga atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa
    10561 cgctccccat caggcggcaa tgaccgcttt agtaaatact cgtcaaaatg tttcccagtt
    10621 tggatcttgt tcagcaattc gcagtcgtgg ctgtgccggt ggttgctcac tggcaggacg
    10681 ggatggtgtt tgcggtttat tggtgagtgg gctggctgcc agacggaacg cggaaaccgc
    10741 ttgcgttaaa cgactcgcct gttcttccag cgcagctgcg gcggcagctg attcctgcac
    10801 cagcgatgcg ttctgttgcg tgacgcgatc catttccgaa accgccaatg cgacttgatc
    10861 gatgccacga ctctgttcat ccgatgctga tgcaatctcg cccataatgt cagtcacgcg
    10921 agtgacagca ttgacgatat tgttcattgt ttcccctgcg ctttcgacca gcaccgaacc
    10981 ggtatcaacg cgcgagacgg agtcttcaat gagggctttg atctcttttg ccgcctgggc
    11041 gctgcgactg gcaagattac gcacttcgcc cgccaccacg gcaaaaccac ggccttgttc
    11101 acccgctcgc gcggcttcaa cagcggcatt cagcgcgagg atattagtct ggaaggcaat
    11161 accatcgata acgctgataa tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat
    11221 cgttttcact acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg
    11281 cgccagttgc gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg cggcgagctg
    11341 ctccatgctg gcggcagttt cttccagcgc ggatgcctgc tgttcagtac gggaggaaag
    11401 atcggtgttg cccgccgcaa tttcacgggt accggcatag atggcatctg aaccttcgcg
    11461 cacatgagtg acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc
    11521 gcccatttca ctgcgcccgt caatggtcag ggtattcgcc aggttaccac cggcaatttc
    11581 gcgaatatgc gcaataattt ttgccagcgg tgtaagcaac atacggcgaa tgccgtacca
    11641 cgccgccagc agaatcaata ccaccaccag cgcgataacc gccagttgcc actgggcaaa
    11701 tcggtaatca tctgcgttgt cagtgacgat atcgcgatac agtttttcac tgctgagggc
    11761 gtactgagca aacgcttctc ccattgcatt ttgcattccc tgggttggct gagcgaaata
    11821 agctccagta ttgccataat caagataatc aatcagttca gttaacgctg tgtgatagtt
    11881 tttatatttt tcatcaatat tacgactggt agcgaccatt tcaggtaacg gtgccatgct
    11941 tttgaatttt ttataatgag tcgcggcctg ggccaatgtt ttcctggcgc tatcgagcaa
    12001 ttcaactttg gcattacttt gctgattgga tgaatccatc atcatccgca ccgctgaacg
    12061 actcaggtta atgcgcgttt gtagcattaa atcccatgtt gacgtcagct cgctctgctg
    12121 ttcccgtaat tgattggaaa ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa
    12181 cagactgccg gaaataagct gtaacagtgc gaataccccc agcaccatta ccaacagcgt
    12241 gactacgcgg atacggttaa tcataaggca ccttcctgaa aacaagttga tctcgttatc
    12301 ggcaaggagg ggggaaactt tattgctgat gccacccgcc gcgaaattga aataaaaaac
    12361 ccgatgcgca gatcatcggg ttcatttcaa ttgaggacat cgggagaatt acgccacttc
    12421 tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc agcagttttt cgatgttcac
    12481 cagaatcaac atgcggtcgc ccagtgcgcc cagtcctgtg agatattcgg ttgaaagcgt
    12541 cacggcaaat tccggtgccg gacgaatttg ctccgccgtc aatgaaagca cgtctgagac
    12601 gccgtcaacc acgatgccga ccacccgctg tccgagattc aggacgataa ctaccgtgtt
    12661 gtcgttatag tccacatcca cctggctgaa cttaattcgt aagtcaacaa tcggcacaat
    12721 aacgccgcgc agattcgtga cgcctttgat aaacgctggc gtgttcgcaa tccgtgtcac
    12781 ctgatcgtag ccacggatct cctgcacttt cagaatatca ataccgtact cttcatcacc
    12841 aagggtaaat accagaaatt cctggcctga cggctcgctg gccagctttg ttacattcgt
    12901 cataccggtc atattgttac ctttttactc attcaggcgg cggtgttcgc catacgttgt
    12961 tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct gccgtcgcca
    13021 agaatggtcg cagcagaaat gccggggact ttgcgatagt tactttcaag gtttttaacc
    13081 acaacctggt gttgaccaat taattgatcc actagcaagg cgtagcggcg accgccactt
    13141 tgtaagatca ccacaattcc ctgggtggct tcggttttcg cgcccgcgac gttgaacact
    13201 ttccacagtt cgacgatagg cagatattca ccccgcactt ccagcacccg ctcgccgcca
    13261 gccagtggat gcagatcggc ttcacggggt tgcagtgatt ccatgacagc attcagcggc
    13321 agaatgaaaa cttcatccgc aacgcgtacg gacatgccgt caagaattgc cagcgtcagc
    13381 ggcagtaaaa tgcggatcgt agtgccagta ccctgcatcg actggatctc gacatgaccg
    13441 cccatctcct ggatattacg tttaacgacg tccatgccga cgccacgccc ggagacgtcg
    13501 gtgacctgct ccgccgtgga gaagccaggt gcaaatatca gcattgcaac ttcgtcgtcg
    13561 ctcatgtttt cgctgacggt cagaccttgc gaggccgctt ttgccagaat tcgttcacgg
    13621 tttagccccg ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg atgttcggca
    13681 gacagaatta aatttccgac gctgttttta cctgcagcga gccgtttttc tggcagttca
    13741 ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat gcgttcaatc
    13801 aggctcttgt cgagttcagt agaactgccc accagcgtca gttctacctg cttgccgagt
    13861 tttcccgcca gatcacgcac cagccgggga tagcgactaa agacatattc catcggcatc
    13921 atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg
    13981 ctggtaatca aatcaccatg attaaccggg tccagttcgc tggaacgctg ggcaagcatg
    14041 gactgggtga tgaccagctc accgacgagg ttaattaatt gatcaacctt ttctaccgct
    14101 acacggatgc tggtggattc actgctgcgc gtcgtttttt cccgctccac gcgaccggtt
    14161 ggcgcttgtt cggctgccag tttaagcatt ggtgaggtgg atatttttgg cgagacgtct
    14221 actgtttcaa aggtaatctg atcggcttca atcacaaaac agagtaccgc tgtgatgtca
    14281 tcttcggcga tgtcgcccgg taatattgcc gagagggaat cagccccttt caccacgtca
    14341 gttaacgttg tcagatgccc cagctcttct tccagcaggt cgacttcgcc ggccttcagg
    14401 cgcgaaagga taattcgtcg cggtgactga ctacgactct gctcatcttg tggttcactt
    14461 ttggcaacca cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc
    14521 agttgacgca aggcctggca gatataatcg aagctggcgg catccggctc ttgcgactgt
    14581 ttataagcgt cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag attgataatg
    14641 tcggtgttga gttgcatctc acctcgtctg gcttcatcga gcaggttttc catcagatgc
    14701 gtggtttcct gcaaaacgct gaagccaaag gttcctgccc ctcctttgat cgagtgggca
    14761 gcccgaaaga tggcattcaa ttgttcggca tctggcgctt ccggctgcaa aaccagcaga
    14821 tgctgctcca tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg
    14881 cttatatcca tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg gaacactgac
    14941 ctgtggtgca acctcaggtt tttccagggc gcttactggc tcattctggc tttcggcgtt
    15001 ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc agcaggctga tgcgacggtt
    15061 gacggcatca tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc cgacgacacg
    15121 taacactttg ccgccattca acccgccaac catcagttcg cggcgggatg cattggcacg
    15181 atcggcagaa agctcccagt tgctgtaacc tttctcaccg ctggcgtagg gaaaatcatc
    15241 ggtatgaccc gagaggctaa ttcggttggg aataccgttc agtacgggcg cgatggcgcg
    15301 cagaatgtcg cgcatatagg gttcgacgtc ggcactgcca gttctaaaca tcgggcgatt
    15361 ctggctatcg atgatctgaa tacgtagacc ttcctggacc agatcgattt tgagatgtgg
    15421 acgtaacgcc cgcagtttcg gatcggactc aatcaactgg tcgagatcgc cacgcaattt
    15481 ccgcaatcga ctttgctcca tgcgtttttt cagctcttcg atgttcggcc gcttattcac
    15541 ttccccctgg ctttgggtgt aatcatcacc accgccggga attgggcttt cactattaga
    15601 aatgcgatcg ccgcccgtaa ccgctgtcgc cagcggagtc cggaagtact ccgcaatctg
    15661 aatcagctct tttggactgg agatagagat cagccacatc accagaaaaa aggccatcat
    15721 tgcggtcata aagtcggcat aagcaatctt ccacgatcca tgtgctgccc cgtggctttt
    15781 ggctttacgt cgtttgacga caataatcgg atgcgcttga ttcttcatgc ttcctcggtt
    15841 gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct cttccagctc aataaacgac
    15901 ggacgttcgc tggaatagag cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa
    15961 ccgttcagat ttgaaagcag agtgactttg acgcactgca tcattttgct ggtttcggcg
    16021 cttttctgac gtaaaacact cgctaatggg gaaataaatc cgtaagccaa taaaataccg
    16081 aggaaagtcc ccaccatcgc atgagcgata agcgccccca gctcggcggc aggacgatcg
    16141 gctgaaccta acgcgtgaac tacccccatt acagccgcaa caataccaaa agccggaagt
    16201 gagtccccga ccagcgccag actgttcgcc gggacttctg cctcgctttc gtgcgtctca
    16261 atttcttcat ccatcagggc ttcgatttcg aaggtgttca tgtgaccgct gataatcagg
    16321 cgcagataat cgacgataaa ttcaagcatg acgctatccg caaggatgcg tgggtagctg
    16381 gcgaagatct cgctctcacg gggattttca atatcgcgtt ccagcgaaaa catccccatc
    16441 tgccgcgatt tcgccatcaa ccgataaagc agagccagca gatccatata cattgctttg
    16501 gtgtatttgg agcgacgaaa cagcaacggc agcgccttca gcgtgccttt aatcgctttg
    16561 ccattattgc cgacgataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg
    16621 ggttgataga gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg
    16681 agaacaacca ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga
    16741 caggatgttc agtcgtcagg cgttaacacg cgataggggc aaaaaaaagc agcggtacgt
    16801 cgttaccgct gctggaatgt ttcgccacac cgtatcagtt aaacagcctg tactctctgt
    16861 tcatccagca gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact
    16921 gcccgggatg gcggctgaca taagctgcag gcaaagctgc caacaggctg gtgagcatgg
    16981 gtaataaaat tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca
    17041 aaccgcacca atgtccaggc acgggttaat gccagcagcg gtccttcttc tgcttgcggg
    17101 cactgttcaa ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaagcca
    17161 gttttcagta aaaactgcca tgcattacag aacatcgaag catgaacatt ttgttcccag
    17221 gtcataaacc agtcggttga aaatggcagc atgcctttcg gcggtgggct tccgcgcagt
    17281 tctttataga gttttatcag acgtccgcga cttaactgtg tttcgctttc cagcatctgc
    17341 aaacgagcgc ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctgaaca
    17401 atgctttttt cactcatgat caggcccttt tcttgcgcag cgcttcttca ggctgattaa
    17461 catcattcag caagcgtgtt gagagcatga tgccggtatg aatttgctgg agatcgtcaa
    17521 cgcgggaatc ttgcgtcaac tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa
    17581 ccagttgatt ggtttctgcc agcttaacca tttgcggaag agtcagtgcc gctaacgttg
    17641 tcgccatttc ttcatttatg ccgagacgaa acatagcgga cgctttgtcc tgaacaatca
    17701 gacgctgtgc aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc agcaactcgg
    17761 aggtatgcat tattcccacc cagaataacc aactttattt ttatgcggtc tcaccgcacc
    17821 ccgtgatgtc gccgggaagc cccggtaaaa aataattagc attagaatag ttgcgataag
    17881 ctgcaataag cagacccacc tttttggttt aataagtcct tacaaataga agggggtctt
    17941 tacacttatc taagattttt cctaaatcga cgcaactgta ctcgtcacta cacgcacata
    18001 caacggaggg gggctgcgat tttcaataat gtgtgatgca gatcacacaa aacgctcaat
    18061 tacttaacat aaatgtataa gtcatacttt tgttttgggt gtatttcaat ctgttaaaaa
    18121 atttttcgct acactggcaa gcaaaaatga aacaggaata atcgaa
//
LOCUS       DACADA010000086        18158 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.087, whole genome shotgun sequence.
ACCESSION   DACADA010000086 DACADA010000000
VERSION     DACADA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.087"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            33..962
                     /gene="pfkB"
                     /locus_tag="ISB17_002919"
     CDS             33..962
                     /gene="pfkB"
                     /locus_tag="ISB17_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAP1980823.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1063..1353
                     /gene="ghoS"
                     /locus_tag="ISB17_002920"
     CDS             1063..1353
                     /gene="ghoS"
                     /locus_tag="ISB17_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP1980824.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            1459..2319
                     /locus_tag="ISB17_002921"
     CDS             1459..2319
                     /locus_tag="ISB17_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP1980825.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2360..2896)
                     /locus_tag="ISB17_002922"
     CDS             complement(2360..2896)
                     /locus_tag="ISB17_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980826.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3043..3711
                     /gene="hxpB"
                     /locus_tag="ISB17_002923"
     CDS             3043..3711
                     /gene="hxpB"
                     /locus_tag="ISB17_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAP1980827.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3875..4465
                     /locus_tag="ISB17_002924"
     CDS             3875..4465
                     /locus_tag="ISB17_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1980828.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4598..5989
                     /locus_tag="ISB17_002925"
     CDS             4598..5989
                     /locus_tag="ISB17_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP1980829.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6036..6299)
                     /gene="cedA"
                     /locus_tag="ISB17_002926"
     CDS             complement(6036..6299)
                     /gene="cedA"
                     /locus_tag="ISB17_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAP1980830.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6482..8743
                     /gene="katE"
                     /locus_tag="ISB17_002927"
     CDS             6482..8743
                     /gene="katE"
                     /locus_tag="ISB17_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAP1980831.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(8790..9548)
                     /gene="chbG"
                     /locus_tag="ISB17_002928"
     CDS             complement(8790..9548)
                     /gene="chbG"
                     /locus_tag="ISB17_002928"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAP1980832.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(9561..10913)
                     /locus_tag="ISB17_002929"
     CDS             complement(9561..10913)
                     /locus_tag="ISB17_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP1980833.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11016..11855)
                     /gene="chbR"
                     /locus_tag="ISB17_002930"
     CDS             complement(11016..11855)
                     /gene="chbR"
                     /locus_tag="ISB17_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAP1980834.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            complement(11866..12216)
                     /gene="chbA"
                     /locus_tag="ISB17_002931"
     CDS             complement(11866..12216)
                     /gene="chbA"
                     /locus_tag="ISB17_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAP1980835.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12267..13625)
                     /gene="chbC"
                     /locus_tag="ISB17_002932"
     CDS             complement(12267..13625)
                     /gene="chbC"
                     /locus_tag="ISB17_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAP1980836.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13710..14030)
                     /gene="chbB"
                     /locus_tag="ISB17_002933"
     CDS             complement(13710..14030)
                     /gene="chbB"
                     /locus_tag="ISB17_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAP1980837.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14329..14667)
                     /gene="osmE"
                     /locus_tag="ISB17_002934"
     CDS             complement(14329..14667)
                     /gene="osmE"
                     /locus_tag="ISB17_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAP1980838.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            14869..15696
                     /gene="nadE"
                     /locus_tag="ISB17_002935"
     CDS             14869..15696
                     /gene="nadE"
                     /locus_tag="ISB17_002935"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP1980839.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            15926..16813
                     /gene="cho"
                     /locus_tag="ISB17_002936"
     CDS             15926..16813
                     /gene="cho"
                     /locus_tag="ISB17_002936"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAP1980840.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(16773..17348)
                     /gene="ves"
                     /locus_tag="ISB17_002937"
     CDS             complement(16773..17348)
                     /gene="ves"
                     /locus_tag="ISB17_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAP1980841.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17551..18036)
                     /gene="spy"
                     /locus_tag="ISB17_002938"
     CDS             complement(17551..18036)
                     /gene="spy"
                     /locus_tag="ISB17_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAP1980842.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         4635 a         4591 c         4299 g         4633 t
ORIGIN      
        1 gccagactga aatcagccta taggaggaaa tgatggtacg tatctatacg ttgacacttg
       61 cgccctcact cgatagcgca acaattaccc cgcaaattta tcccgaagga aaactgcgct
      121 gtaccgcacc ggtattcgaa cccggaggcg gcggcatcaa cgtcgcccgc gccattgccc
      181 atctgggagg cactgccaca gcgatcttcc cggcgggtgg cgcgaccggc gaacatctgg
      241 tttcactgtt ggcggatgaa aatgtccccg tcgctactgt agaagccaaa gactggaccc
      301 ggcagaattt acacgtacat gtggaagcaa gcggtgagca gtatcgtttt gttatgccag
      361 gcgcggcatt aaatgaagat gagtttcgcc agctcgaaga gcaagttctg gaaattgaat
      421 ccggggccat cctggtcata agcggaagcc tgccgccagg tgtgaagctg gaaaaattaa
      481 cccaactgat ttccgctgcg caaaaacaag ggatccgctg catcgtcgac agttctggcg
      541 aagcgttaag tgcagcactg gcgattggta acatcgagtt ggttaagcct aaccacaaag
      601 aactcagtgc gctggtaaat cgcgaactca cccagccaga cgatgtccgc aaagccgcgc
      661 aggaaatcgt taatagcggc aaggccaaac gggttgtcgt ttccctgggt ccacaagggg
      721 cgctgggcgt tgatagtgaa aactgcattc aggtggtgcc accaccggtg aaaagccaga
      781 gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac acttaaactg gcagaaaatg
      841 cttctcttga agagatggtc cgttttggcg tagctgcggg gagtgcagcc acactcaacc
      901 agggaacacg cctgtgctcc catgacgata cgcaaaaaat ttacgcttac ctttcccgct
      961 aacaaaaaca ttcccccagc attgggggaa tcaccaccaa cctgtcggca acgcgtttct
     1021 ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa ctatggccag tggcgatctt
     1081 gtccgttatg taataaccgt catgttgcat gaggatacat tgactgaaat taacgagttg
     1141 aataattacc tgactcgcga cggttttttg ctcaccatga cagatgatga tggcaatatc
     1201 catgagctgg ggactaacac ttttggactt atcagtaccc aaagtgaaga agaaattaga
     1261 gaactggttt cggggcttac ccacagtgca accggcaaag atcctgaaat caccatcacg
     1321 acctgggagg aatggaatag caacagaaaa taaatggttt ttgggcaata atcagtctgt
     1381 ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc taaccttatg atctggcaga
     1441 caacatggga gagacatcat gtggcaggca atcagtcgtc ttttgagcga gcagttaggt
     1501 gaaggcgaaa tagaactgcg taatgaactg cctggcggag aagtccatgc cgcatggcat
     1561 ttgcgctatg caggacgtga ctttttcgtc aaatgtgatg aaagggaact gcttcccggt
     1621 tttaccgccg aagccgacca actggagtta ctgtcgcgta gtaaaaccgt caccgtgcct
     1681 aaggtttggg cagttggcgc tgaccgtgac tacagttttc tggtgatgga ttatctccca
     1741 cctcgcccgc tggatgcgca tagtgcattt attcttggtc agcaaattgc gcgtttacat
     1801 caatggagcg accaaccaca atttggcctc gatttcgata acgcgctctc cacaactcca
     1861 cagcccaaca cctggcaacg tcgctggtca actttttttg ctgaacaacg gattggctgg
     1921 cagctggaac tggcggcaga aaaagggatc gctttcggca atatcgatgc catcgtcgag
     1981 catattcagc agcgtcttgc ctcgcatcaa ccgcagcctt ctctgttgca cggcgattta
     2041 tggtccggca actgtgcgct gggtccggat ggcccgtaca ttttcgaccc ggcctgttac
     2101 tggggtgacc gagagtgcga cctggcgatg ttaccgctgc atactgaaca gccgccacaa
     2161 atctatgacg gctatcagtc agtatccccg ctacctgccg atttccttga acgtcaaccg
     2221 gtttaccaac tctacacgct gttaaatcgt gcaaggttat ttggcggtca gcatttggtt
     2281 attgctcagc agtcactgga tagattatta gcagcatgat atgggttgag gataatggcc
     2341 gctccgtgcg gccttttgat taaataaacc caagcagaga aaagaacaca taacctgcag
     2401 caatacaaat aaccggcagg atatacagcg agaaaaactg caggaaaata gtatggtgcg
     2461 gaacaacaat acgtgactca atttgttcac gcgttagccc ctcttcccct ttggcttttt
     2521 ccagaatgag ttgatcttca acaccttccc gcaagaagcg cgcctggcga ctcatccggg
     2581 ctccggaatc ctgcaacgcc agtccgacaa aaatcaggat gaaaatcacc cagaacataa
     2641 cgttcacgcc accattaaaa ttgggcgtcg gagagttgta ccagaacaga ttcaaaaaag
     2701 gcgtattcgc ctgcatcata tcgatcatga catgagtaaa gtcgagcatg accgcattaa
     2761 tgccttcctg gttttcctgc cgggtattca taaatttcag caaagaaatc aatgtagaga
     2821 tcaatgcagg tataaaaatc acccaaccca aaatcctttt caaaacagca atgcgtccag
     2881 cttgttgata cgtcatgagt tctccttgat taagacgcgt cgtttcactt agtttacctg
     2941 tagatatctg ttttcgccca ttctttaaag gcgatatgat aggcgcttaa tcataagcac
     3001 ggcttaatac cttacatata atgctctaaa ggagaggttg taatgtcaac cccgcgtcag
     3061 attcttgctg caatttttga tatggatgga ttacttatcg actcagaacc tttatgggat
     3121 cgagccgaac tggatgtgat ggcaagcctg ggggtggata tctcccgtcg taacgagctg
     3181 ccggacacct taggtttacg catcgatatg gtggtcgatc tttggtacgc ccggcaacca
     3241 tggaatgggc caagccgtca ggaagtagta gaacaagtta ttgcccgtgc catttcactg
     3301 gttgaagaga cacgtccatt attgccaggt gtgcgtgaag ccgttgcgtt atgcaaagaa
     3361 caaggtttat tggtggggct ggcctccgcg tcaccgctgc atatgctgga aaaagtattg
     3421 accatgtttg atttacgcga cagtttcgat gccctcgcct cggctgaaaa actgccttac
     3481 agcaagccgc atccgcaagt atatcttgac tgcgcagcaa aactgggggt tgaccctctg
     3541 acctgcgtgg cactggaaga ttcggtaaat ggcatgatcg cctctaaagc agcccgcatg
     3601 cgttccatcg tcgttcctgc gccagaagcg caaaatgatc cacgttttgt attagcaaac
     3661 gtcaaactct catcgctgac agaactcacc gcaaaagacc ttctcggtta atgaccaggg
     3721 ggcagtgatc acactgcccc tggttcttta tctgaatttg cattcaaatg acggattaat
     3781 cgccaattta agagaaagag ttacaccgtc accacttccg tgcactgtat aaaaacccta
     3841 tactgtacgt atcgacagtt tagtgagttt tatcatgacg gcggaaggtc accttctctt
     3901 ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc tggcacaggg
     3961 ggactggtgg catattgtcc cttccgcaat cctgacgtgt ttgttaccgg acatcgatca
     4021 cccaaagtcg tttcttgggc agcgattaaa gtggatatca aaaccgatcg cccgcgcttt
     4081 tgggcatcgt ggttttaccc acagcctgct ggcggtattt gcgctgctgg caacctttta
     4141 ccttaaggtt cctgaaagct ggttcattcc ggctgatgcg ttgcagggta tggtgctggg
     4201 ttatttgagc cacatacttg ccgatatgct gacacccgcc ggtgttcccc tgctctggcc
     4261 atgccgctgg cgtttccgct tgcctatcct ggttccccaa aagggcaacc aactggaacg
     4321 ttttatctgc atggcattat ttgtctggtc ggtatggatg ccccattcat tacccgaaaa
     4381 cagcgctgtt cgttggtcat cgcaaatgat caatacctta cagatccagt ttcatcggct
     4441 tattaagcat caggttgaat actaaaaagg caaaaatcgc ctttctggaa taagcaattc
     4501 catttgaata taagagccag ctcacagttc tgttaatctt gcgccaacac tatgactgct
     4561 acgcagtgat agaaataata agatcaggag aacggggatg aactttccat taattgcgaa
     4621 catcgtggtg ttcgttgtac tgctgtttgc gctggctcaa acccgccaca aacagtggag
     4681 tctggcgaaa aaagtgctgg tgggtcttgt gatgggtgtg gtttttggcc ttgccctgca
     4741 taccatttat ggttctgaca gccaggtact taaagattct gtacagtggt ttaacattgt
     4801 tggtaacggc tatgttcaac tgctgcaaat gatcgttatg ccgctagtct tcgcctctat
     4861 tctgagcgcg gttgcccgtc tgcataacgc atctcagtta ggcaaaatca gttttctgac
     4921 catcggtacg cttttgttta ccacgctgat tgcagcgctg gttggtgtgc tggtcaccaa
     4981 cctgtttggt ttgacggctg aaggtctggt tcagggtggt gcagaaactg cacgtctgaa
     5041 cgccatcgaa agtaactatg ttggtaaagt ctctgacctg agcgttccgc agctggtctt
     5101 gtcctttatc ccgaaaaacc cgtttgccga tctgaccgga gccaatccga cgtcaattat
     5161 cagcgtggta atttttgccg cattcctcgg cgtagctgcg ctgaaactgc tgaaggatga
     5221 tgcgccgaaa ggtgaacgcg tcttaaccgc tatcgatacc ctgcaaagct gggtgatgaa
     5281 actggttcgc ctggtcatgc agttgacccc ttacggtgtt ctggcgctga tgaccaaagt
     5341 ggttgcaggt tctaacctgc aagacatcat caaactggga agtttcgttg tcgcgtccta
     5401 cctcggtctg ctgattatgt ttgcagtgca tggccttctg ctgggcatta atggcgtgag
     5461 cccgctgaag tacttccgta aggtatggcc agtactgacg tttgccttta ccagccgttc
     5521 cagtgctgcg tctatcccac tgaatgtgga agcacaaacg cgtcgtctgg gtgttcctga
     5581 atccatcgcc agtttcgccg cctctttcgg tgcgaccatt ggtcagaacg gttgtgccgg
     5641 tttgtatccg gcaatgctgg cggtgatggt tgcgcctacg gtgggcatta acccgctgga
     5701 cccgatgtgg attgcgacgc tggtcggtat tgtcaccgtt agttccgcag gcgttgccgg
     5761 tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg gcctgccggt
     5821 aacactggtg gcattgttaa tctccgttga accgcttatc gacatgggcc gtacggcgct
     5881 aaacgttagt ggctcgatga ccgctggcac gctgaccagc cagtggctga agcaaaccga
     5941 taaagccatt ctggatagcg aagacgacgc cgaactggca caccgttaat cctgatttgt
     6001 tacgttaaac gcctgtcatc ccgacaggtg tttttttatt cttcgacctc attttcctgg
     6061 cggatctgat ttgcccaacg ctgggcggat tcaggcacag taaacgccgg tgagcgcaga
     6121 aatgactccc ccatcagtac aaacgcaaca tatttgccac gcagcatcca gacatctcga
     6181 aacgaatcca tctttatcgc atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta
     6241 ataatctggc ggttttgctg acggagcggt ttcttcatta ctggcttcac taaacgcata
     6301 ttaaaaatca gaaaaactgt agtttagccg atttagcccc tgtacgtccc gctttgcgtg
     6361 tatttcataa caccgtttcc agaatagttt ccgaagcggg atctggctgg tggtctatag
     6421 ttagagggtt ttttgaccaa aacagcggcc ctttcagtaa taatttaagg agacgagttc
     6481 aatgtcgcaa cataacgaaa agaacccaca tcagcaccag tcaccactac acgattccag
     6541 cgaagcgaaa ccggggatgg actcactggc acctgaagac ggatctcatc gtccagcggc
     6601 tgaaccaaca ccgcctggtg cacaacctac cgccccaggg agcctgaaag cccctgatac
     6661 gcgtaacgaa aaacttaatt ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct
     6721 gaccactaat cagggcgtgc gcatcgccga cgatcaaaac tcactgcgtg ccggtagccg
     6781 tggtccaact ctgctggaag attttattct gcgcgagaaa atcactcact ttgaccatga
     6841 gcgcatcccg gaacgtattg ttcatgcacg cggatcagca gctcacggtt atttccagcc
     6901 atataaaagc ttaagcgata tcaccaaagc ggatttcctc tcagatacga acaaaatcac
     6961 cccagtattt gttaggttct ctaccgttca gggcggcgct ggctctgccg ataccgtgcg
     7021 tgatatccgt ggctttgcca ccaagttcta tactgaagag ggtatttttg acctcgttgg
     7081 caataacacg ccaatcttct ttatccagga tgcgcataaa ttccccgatt ttgttcatgc
     7141 ggtaaaacca gaaccgcact gggcagttcc acaagggcaa agtgcccacg atactttctg
     7201 ggattatgtt tctctgcaac ctgaaactct gcacaacgtg atgtgggcaa tgtcggatcg
     7261 cggtatcccg cgcagttacc gcactatgga aggcttcggt attcatacct tccgcctgat
     7321 taatgccgaa gggaaggcaa cgttcgtacg tttccactgg aaaccactgg caggtaaagc
     7381 ctcactcgtt tgggatgaag cacaaaaact aaccggacgt gacccggact tccaccgccg
     7441 cgagttgtgg gaagcgattg aagcaggcga ttttcctgaa tacgaactgg gcttccagtt
     7501 gattcctgaa gaagacgaat tcaagttcga cttcgatctt ctcgatccaa ccaaacttat
     7561 cccggaagaa ttggtgcccg ttcagcgtgt cggcaaaatg gtgctcaatc gtaatccgga
     7621 taacttcttt gctgaaaacg aacaggtggc tttccatcca gggcatattg tccccggtct
     7681 ggatttcacc aacgacccgt tattgcaggg gcgtttgttc tcttataccg atacacaaat
     7741 cagtcgtctt ggtgggccga atttccatga aattccgatt aaccgcccga cctgccctta
     7801 ccataatttc cagcgtgacg gcatgcatcg tatggggatc gacactaacc cggcgaatta
     7861 cgaaccgaac tcgatcaacg ataactggcc gcgcgaaaca ccgccggggc caaaacgtgg
     7921 cggttttgaa tcataccagg aacgcgtgga aggcaataaa gttcgcgagc gcagtccatc
     7981 gtttggcgaa tattattccc atccgcgtct gttctggcta agtcagacgc cattcgagca
     8041 tcgccatatt gtcgatggtt tcagttttga gttaagcaaa gtagttcggc cgtatattcg
     8101 tgagcgcgtt gttgaccagc tggcgcatat tgatctcact ctggcccagg cggtggcgaa
     8161 aaatctcggt atcgaactga ctgacgacca gttgaatatc accccgcctc cgaacgtcaa
     8221 cggtctgaaa aaggatccat ccttaagtct gtacgccatt cctgacggtg atgtgaaagg
     8281 tcgcgtggta gcaattttgc ttaatgatga agtgagatcg gcagaccttc tggccattct
     8341 caaggcgctg aaggccaaag gcgttcatgc caaactgctc tactcgcgaa tgggggaagt
     8401 gactgcggat gacggtacgg tgttgcctat agctgctacc tttgccggtg cgccttcgct
     8461 gacggtcgat gcagtcattg tcccttgcgg caatattgcg gatatcgcta acaacggcga
     8521 tgccaactac tatctgatgg aagcctacaa acaccttaaa ccgattgcgc tggcaggaga
     8581 cgcgcgcaag tttaaagcaa caatcaaggt cgctgaccag ggtgaagaag ggattgcgga
     8641 agctgaccgc gccgatggta gttttatgga tgaactgtta acgctgatga cagcacaccg
     8701 cgtgtggtca cgcattccta agattgacaa aatcccggcg taaatatgga taaggtcacg
     8761 ccccctgggg agtgacctta ccaccttact tacacatcat gataactacc caaccgataa
     8821 ccacgctcag caatcgcata tttcaacgac gctgaagtca gcacatccag ttccgttaac
     8881 cgagggaagc aataagcact ctggcgaatc gtattatcga taaacgccgg gtggcacatc
     8941 acttccagcg agcgctcgcc gcgatgactg gagtcatcaa gcacttgcag gaacagtgtc
     9001 tcgctaatct cttcaccata aaacgcgcta ctgaatccct gcgaactgcg cagattagcc
     9061 ggtaaatcac catccttaga aagcgactga cggtcgatgc gcaacgcaat cccctcttca
     9121 gccgcaaact ttgccacaat cgggaaaatc tgcgggaaca tatgcacatg atgatggcta
     9181 tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg actggcgagc
     9241 tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca gatccatttt
     9301 cccagcacac catcgcgggt taaccccggc atagctgtca gaggcttgcc catagtaagg
     9361 acaaagtgca tccctatggc cagactcggt tcatcacggc tcaactgcac cgcatggtca
     9421 atagcctgcc cattcaccag cgccgtcgtc gacgtgacaa tcccattgcg acaggcctcg
     9481 ataatgccgt agttctgacc tttgcttaag ccaaaatcat cagcattaac aatcagtaag
     9541 cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa
     9601 tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc agcatcgcga
     9661 tcggaatgca ccaacggact aaggtttagc gccagtaaga catcgttaaa ttctccacta
     9721 agtgcggcgt tgctggcagc aatctcgaag cctttgatgg tgtgaatcag ccccatcact
     9781 ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc cagcgtacag
     9841 gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg cgggatatta
     9901 acgtaatgtt ctgcttgctt gtcgttatag atagcgttga tcacttcgca cgcagcatca
     9961 gaataataag ccccaccgcg ctgttccagt tctttcggct taactttcaa ctccgggttt
    10021 ttatatagct caaaaagttg tttctcgact ttctgtacga cctgcgctcg tgcgccgcct
    10081 ttgtagtatt cgcccatttc aatagccagc atctcttttt gcttgaagta ataaagcaaa
    10141 taagaacacg gcaacagatt cagagaacga attaagcctt cgctaaatgg cagatcgaaa
    10201 atatttttaa cgccagatgc ttttaactgc ccggatgcca caccatcaag caattcggca
    10261 aagcgtgatt tgccatttac cagcacatcc ttaatgaaca ccatatggtt gaggccgaac
    10321 agatcgatag ataaatcatc gctgtctttc agcatcagaa catcgcgaat aaacatcttc
    10381 atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg acgataaacg
    10441 gcttcagtga ccattcccgc cgggttagtg aagttaatca cccatgcatt cggacatagt
    10501 tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc tttaaacaga
    10561 ccgcccgcgc cgttagtttc ctgaccaaga taaccatgac ttaatggaat acgttcatcc
    10621 agttcccgcg ccggtaattg gccaacgcgc agttgggtag taacgaaatc agcatctttc
    10681 aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc gttatcaatc
    10741 atccgttggc agagatcaaa aataatatcc agtttcgctt taccaccttc gacatccacc
    10801 agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc cagtaactcc
    10861 ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct cataatttct
    10921 cccttcagta cagaatactg atatctggca tatctgcccc cggacataaa taatccagca
    10981 acaggacaga tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc
    11041 ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaagctc
    11101 gggctactat aaccggcctc aaacgcaata tccgtcactg aatagttggt catttccagt
    11161 tgttttttgg caaaattaat acggatttca ttaataatct gcattggcgt tttaccataa
    11221 tatcgttgag tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc
    11281 tccagcgccg attcactaaa ctgctcttta tcatgcattt tttctaccgt acttttcagc
    11341 cactgcggta tatcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca
    11401 taaaaagtaa ccatctcaac aaactcttcc agccctgttt cgcggaaatt caatgaagaa
    11461 atcactgttt ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagctaca
    11521 aagcaataag gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg
    11581 ttcaatatac gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata
    11641 aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc
    11701 ccggttaata ccagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca
    11761 gttttattat agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca
    11821 gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt
    11881 ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg
    11941 atcctgagcg tggaccagca ccagactcac tttcatctta ccttcgccag catcgccttc
    12001 aatcagtttc gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat
    12061 cgcttttgct gcggcaaaat cgccctgctt cgcctgtttc agtgccgcat acgccaggct
    12121 acgcgcttgt ccggagttga tgataagccc catcaccact tcttccagct cttcagcttc
    12181 cgtttgcgta tcgggaatat tatcgagatc catcatacat cgttcctctc tattcttacc
    12241 ggcacgatta cccgtaccgg catcgattaa aatttcaggg cgttagcgat atcttcttcg
    12301 ctctcttctt tatcaatcgc gttttgtgct ttgttagcga ccacaacaaa gggcagataa
    12361 attaacgttg cgataccaag gttgaagagt gcgaccagca atgcggcgac gctaccgttg
    12421 gtgttaaaga aggctcccag accggttggc atggtccacg gtgcaatatt ggtcaccggt
    12481 ggaataatgc ccatgtagta cgctgccagg gtgattgccg ccagaatcgg ttgtaccagt
    12541 acaaacggga taaacatcac cgggttcatg ataattggca gaccaaacag aatcggttcg
    12601 ttaatctgga agatgccaga cggcagcgcc agttttgcca cctgacgata atcagcacgt
    12661 cgagaggcga taaagatagc caggatcagg cctaaagtcg caccactgcc accaaggaaa
    12721 ataaaggagt ccagcatcgg cttggcccag atatggaagg tcttaccggc tgccagcgcc
    12781 gcttcgacgg aaccatattg ctgataggtc gcgatatttt ccagcgccca aggcgtcata
    12841 atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa ccagagcagt
    12901 ggaacaaaga tcacataggc ccagcctacc acgctaccca acgatgccag tggggttgag
    12961 atcgtatcca taatgatctg atggaagttg gtgccccagg tattcaacgc ccaggcaata
    13021 atccccatca cggaaagaat aataaaacca ggaattaatg ctgagaaaga gcgcgaaacc
    13081 gatgtcggaa cgctatcagg taacttaatg acccagttac gatgaacaat aaaggtaaac
    13141 atttctgcca ccaccaggcc aataataatc ccggagatga tattcgcccc acctaaccag
    13201 tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg tgacggtcat aaatgctgca
    13261 acggataaca acccggccgc cagcgcatcg actttacgct cttctgccag cgccatgcca
    13321 ataaagaacg gtgccattaa agacattatc cctaatgttc cgttatatac gttgccgcca
    13381 atacctttca gaccattaag tgtttcaatg gttgaggcat ccaggcgaat acctaaggaa
    13441 taaaaaaacg acccctcccc aaagcttaga aaaacgttgt taattaatac aaacatggcc
    13501 cccgcaaggg ttaacggcat taagcgaata aagccatttt tgattgcatt aacgtgtggc
    13561 tgctttccta ttttaactgc aaaagggagg agtacctttt caagcgatgc aataacatta
    13621 ctcatagaaa aataccctta aaaaccgcaa tttaaatatt gcggtattga tttatgaaat
    13681 aactctttga cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca
    13741 accgcagcct taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact
    13801 tcaaccggtt tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc
    13861 cctaataaca cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct
    13921 tcaataatga ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa
    13981 gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc
    14041 tcatcgacga ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg
    14101 ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtataggcc
    14161 tgagcaatag tatttctttg acctgataaa ttgtctctcc ttgacctgtg gttatgaccc
    14221 tcttcacatt tcgggcaaat aattcgcgaa gataattaag aacacaggcc aaaaaaaacc
    14281 ggcgcaatgg ccggtttccg ttgttactca agctttcagg cgaagtgatt acttcgtagc
    14341 ctgcggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt
    14401 atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca ggatgtaggt
    14461 ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg
    14521 tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg
    14581 gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact
    14641 cagaattcct gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata
    14701 agcctggaat acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag
    14761 gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca
    14821 acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa
    14881 caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga ggaaattcgt
    14941 cgtagtatcg attttctgaa aagctacctg caaacttatc cgttcattaa atcactggtg
    15001 ctcgggatca gcggcggtca ggactccacg cttgccggta agctgtgcca gatggcgatt
    15061 aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccat acgcctgccc
    15121 tatggtgtcc aggctgacga acaagattgc caggatgcca ttgcctttat tcaaccggat
    15181 cgcgtattaa ccgttaatat caagggcgcg gtactcgcca gcgaacaggc gctgcgcgaa
    15241 gcgggcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa
    15301 gcacaatata gcattgcggg tatgaccagt ggtgtcgtgg tgggcaccga tcatgcagca
    15361 gaagctatta ccggattctt cactaaatat ggtgacggcg gtacggacat taatccgctg
    15421 tatcgtctca acaaacgtca gggtaaacag ttactggcgg cattaggttg cccggaacac
    15481 ctttataaga aagcgccaac ggccgatctt gaagatgatc gcccttctct accagatgaa
    15541 gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacgtacct
    15601 gaacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg
    15661 ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacctat tggattgtgc
    15721 gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg ccttactcga cctacaaaac
    15781 tatgcaaatt caatacattg caggagtcga ataggcctga caggcgtagc acgtcagacg
    15841 gtgtaacctt tgtcatcaac ccgcctcttt tttaatcgct ccccgcctgt tacactggat
    15901 agataaccag cattcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt
    15961 tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc
    16021 cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc tctatatcgg
    16081 caaaagcgtt aatatccgca gtcgggtgct ttctcattta cgcaccccgg atgaagccgc
    16141 catgctgcga caatcccgac gcatcagctg gatatgtacc gccggtgaaa tcggcgctct
    16201 actccttgaa gcgcgattaa tcaaagagca gcagccgctg tttaataaac gcttgcgccg
    16261 caatcgccag ctctgcgccc tgcaattaaa tgaaaagcgt gttgatgtgg tgtatgccaa
    16321 agaggtggat ttttcacgag cctccaacct gtttggcctg tttgccaata ggcgcgcagc
    16381 tttgcaagca ttgcagacca tcgccgatga acaaaaactt tgttatggcc tgttgggact
    16441 ggaaccgtta agccgcggtc gtgcatgttt tcgttctgca ttaaaacgtt gcgccggggc
    16501 gtgttgcggt aaagagagcc atgaggaaca tgcgctacgc ttgcgccagg ctctggagcg
    16561 tttgcgggtg gtgtgttggc cttggcaagg cgcggtggca ttgaaagaac agcacccgga
    16621 aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcactgaa
    16681 agaagcgaca acgttaattc gggcacccgc cgggtttgat cacgacggtt ataaaattct
    16741 ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg tgaatgacca
    16801 gcaagccagt tgatttcact gaacaacagt ttaccttgcg gttgcaaaag ccgcagggtg
    16861 tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc gcccaattgc
    16921 cacgccccgt ttattacaaa caccaccccg ccgcgtgaac caaaggtggt aaacgtacgt
    16981 tcggcaattc tgactttcgc cttacagaca tccaggcgtg tcatgatgtt gaaatccatc
    17041 gacatctgcc ctgccgtcag tttcgctttt accaccagat ccgctgcaaa ggaaaaaggt
    17101 tgtaacggtt ttaaggtatg gttaaagcgg tctgcgcttt caaggaacat ctcgccgcct
    17161 tccagcaacg tcactatcct ttccataccg ggaaacaaag aaaactcccc attcgccgcg
    17221 atggatgtaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt
    17281 tcgcgcgttt caccggcagc gtttcgccac agattcaccg acattttacg catatcaaag
    17341 tattccatac ccgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg
    17401 gttatcggca actcgggtga tttacgttag tggcgatcag gctttttttg catctgtttg
    17461 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga
    17521 ccggcggtct tacgtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt
    17581 tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc
    17641 cggcgtcagg atgttgtaaa tcttgttctg ggtttccatg tgcgccagca tgttagcttt
    17701 gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct
    17761 ggcaatgatg tcatgcattg cgcggcgttc ttccagtggc ggacgtttca tctggtcacg
    17821 ctggcctttc atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt
    17881 gaacatcatg tcctcatgcg gaccgaactt gcctttatgg tgcatcatcg gcttcgcgtc
    17941 agccggtgct gcagtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag
    18001 ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta
    18061 tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag
    18121 cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtg
//
LOCUS       DACADA010000087        18113 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.088, whole genome shotgun sequence.
ACCESSION   DACADA010000087 DACADA010000000
VERSION     DACADA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.088"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            114..1100
                     /locus_tag="ISB17_002939"
     CDS             114..1100
                     /locus_tag="ISB17_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP1980843.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGEEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEADQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            1145..1648
                     /locus_tag="ISB17_002940"
     CDS             1145..1648
                     /locus_tag="ISB17_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1980844.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            1648..2949
                     /locus_tag="ISB17_002941"
     CDS             1648..2949
                     /locus_tag="ISB17_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP1980845.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLITLMMITYIPQISLFIPQLLGLM"
     gene            complement(3005..4012)
                     /locus_tag="ISB17_002942"
     CDS             complement(3005..4012)
                     /locus_tag="ISB17_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAP1980846.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVSIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(4129..5175)
                     /gene="ansB"
                     /locus_tag="ISB17_002943"
     CDS             complement(4129..5175)
                     /gene="ansB"
                     /locus_tag="ISB17_002943"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAP1980847.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(5351..6070)
                     /locus_tag="ISB17_002944"
     CDS             complement(5351..6070)
                     /locus_tag="ISB17_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAP1980848.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(6254..6580)
                     /locus_tag="ISB17_002945"
     CDS             complement(6254..6580)
                     /locus_tag="ISB17_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAP1980849.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            complement(6580..7299)
                     /gene="trmB"
                     /locus_tag="ISB17_002946"
     CDS             complement(6580..7299)
                     /gene="trmB"
                     /locus_tag="ISB17_002946"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP1980850.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            7460..8512
                     /gene="mutY"
                     /locus_tag="ISB17_002947"
     CDS             7460..8512
                     /gene="mutY"
                     /locus_tag="ISB17_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP1980851.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8540..8815
                     /locus_tag="ISB17_002948"
     CDS             8540..8815
                     /locus_tag="ISB17_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAP1980852.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            8880..9959
                     /gene="mltC"
                     /locus_tag="ISB17_002949"
     CDS             8880..9959
                     /gene="mltC"
                     /locus_tag="ISB17_002949"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAP1980853.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            10161..11417
                     /locus_tag="ISB17_002950"
     CDS             10161..11417
                     /locus_tag="ISB17_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAP1980854.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11466..13601)
                     /locus_tag="ISB17_002951"
     CDS             complement(11466..13601)
                     /locus_tag="ISB17_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP1980855.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFCVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            13994..14701
                     /locus_tag="ISB17_002952"
     CDS             13994..14701
                     /locus_tag="ISB17_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP1980856.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14807..14882
                     /locus_tag="ISB17_002953"
     tRNA            14807..14882
                     /locus_tag="ISB17_002953"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14840..14842,aa:Phe,seq:gaa)
     gene            15080..16345
                     /locus_tag="ISB17_002954"
     CDS             15080..16345
                     /locus_tag="ISB17_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAP1980857.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            16601..17644
                     /locus_tag="ISB17_002955"
     CDS             16601..17644
                     /locus_tag="ISB17_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP1980858.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWILPF
                     SAAGMKHTGIQYQ"
BASE COUNT         4498 a         4543 c         4299 g         4773 t
ORIGIN      
        1 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaagcactg
       61 ataagcacaa ttagatatca accaatttcc attcttcaac agggagtttt tttatgaaaa
      121 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
      181 taaacgccgc agaaaaagtg acgttaaagt tagcacacaa cctggaaaga agtcatgttg
      241 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
      301 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gagttgttgc
      361 aaaatggtgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
      421 tttatgctat ctataatttg ccgttcttat ttaaagatca ggcgcacttc aacaaagtgg
      481 tttttggtga agttggtgaa gaaattatgg attcaacaaa agataaaggc ttttttgctt
      541 tatcagctta tgtagcgggt acacgcagtt tctatgcgaa aaaaccgatt actaagccag
      601 aagatcttaa agggctgaaa attcgtgttc agccaagccc gaccaccatc aaaatgattg
      661 aactgatggg tggctcaccc actccaattt catttggcga agtttatact gcgatgcagc
      721 aaggtgtagt agatggcgca gaaaataacg tgccttcctg ggtgcagact agacatatcg
      781 aaattgccaa agtcttttct gaagatgaac atgcctcaat tcctgacttt ttggttatct
      841 ccaccaaaac atggaataaa ttaaccccag aacagcaaca aatcctcgaa acagcggcga
      901 agaaatccga agctgatcaa caaaaattgt gggaaaaaat tgacgctgac acacgtgctc
      961 aggcgaaagc tatgggtggt gaaatcgtta aggttgataa agccccattc cgtgccgcag
     1021 ttcaacctct gtttgacgac ttcaaaaaag atccaaaaca agctgctttg ttagagaaat
     1081 tcgacaacgc tgctcaataa tatttcgggc cagtctctgg ccctgatttt ccaggatgct
     1141 tacaatgata tttaaccgcc tgaaacttgc ggtagaccgc gtcattgccg cgttttctgt
     1201 cgccgtaatg cttgctctgg ttgtatgcgt agtctggcaa gtatttagcc gctacgtact
     1261 taaccagcca agtacattaa ccgatgagct ggcacgcttt ttgatgatct gggtaggatt
     1321 attaggtgct gcatataccg tcggcgcgca gagacatctc tctatcgatt tatttgctct
     1381 cgccctgaat aagcgcaagc aattacttct tagtatagtc attaacgttc ttattttagg
     1441 ttttgccggt tcggttattg tgactggtgg cctgaaatta attgataaaa cactggcaac
     1501 atcacaggta tctgccgcga tgcaaatacc gatgggatac gtatatatca ttctgccatt
     1561 aagtggattg gtgatgatgt tttatgcctt gtgttttatt aatcaaagca ttcaacaact
     1621 gaaacaacct gcgcaggagg ccagttaatg gatagttata tcgcgcttac actgtttggc
     1681 tgtttttttg tgctggtctt tattggtgtg cccatttcgt tctcaatcgg tattgccact
     1741 gtagcttcaa tgctgttgat gtttccctgg gatatcgcgg caatcactgt ttcgcagcga
     1801 ctggctaatg gactggataa ctttgcccta ttggcaatcc cctttttcat ctttgccgga
     1861 acgctgatga acagcggcgg tattgcgata cgtttgatta acctcgccca ggtaatggtt
     1921 ggccgcgttc ctggttcgct tggtcatgtg aacgtcctgg cgaatatgat gtttggttcc
     1981 atttctggtt cagctgttgc ggcagctgct gccgttggtg gcacactgaa tcccattcag
     2041 accaaagaag gttatgatcc ggcattttct accgctgtaa acgtctcatc ttgtattact
     2101 ggattactga taccgccctc aaacgtgtta attgtgtttt ccctgacagc tggcggcgtt
     2161 tctgtagcat cgctatttat ggcgggatat ttgccaggga tcttaatggg tctcgcggta
     2221 atgatagtct gcggcattat tgctaaacgc cgtggctacc cactttcaga aagagcaacc
     2281 tttgctcagg catgtaaagc gtttctggat gccttaccaa gtttgcttct ggtgttcatt
     2341 gtaatgggcg gcattctggg cggcattttt accgcaactg aagcgtcagc aattgccgtt
     2401 gtctacacat ttattctctc ggtgctgatt taccgggaag taaaatggcg cgatttaccc
     2461 aaactgattc tggaatcggt tgttaccact tcgattgtat tactgttgat cggtttttcc
     2521 gtgggcatgt cctgggcaat gaccaatgct gatattcctt atatgatcag cgacgcgttg
     2581 atggggattt ctgataaccc actggtcata ttgctgctga ttaatatcgt cttattaatt
     2641 gtcggcatct ttatggacat gacaccggct gtactaatct tcacaccaat cttcttgccg
     2701 attgcacaag agctgggaat ggatccggta catttcggta ttatgatggt ggccaacctt
     2761 tgtattgggc tgttaacacc gccggtgggt agtgcgctat ttgttggttg ttctatttca
     2821 ggtgtgaaga ttcaacattt gatcaaacca ttgctgccat tttatgccgc attgttaatc
     2881 acgttgatga tgatcactta catcccgcaa atatccttgt tcattccaca gctactaggg
     2941 ctaatgtaaa aaataatttg ccggataact ttttatatta tccggcaaag gtacttaaca
     3001 tgagttactt aatcactcca ttgcgccgca attgttcggt atcttttttc cagcgttcat
     3061 tagcctgatt gatatcgtca attttcatca aaatcgcgac ttttttactg tttatttctg
     3121 aaactttcat cgttaacgga tattgccctt tctcgagcgt tatccagtca tcctgcaatt
     3181 tacggctaag tttttgtgat tgcagaaaag tctgtcgccg ttgttcgttg atatcgaaat
     3241 ctcgcttctg ctgaatcgac acttcataac actcttcact ctgtttatga catgccgcaa
     3301 tcgccggttg ataaacctct ttgtcatatt taaccgcgaa cgcctcgcta tcattctgtt
     3361 tattaaactc atcacgctgt tggtgaattt ttttaaaggc gtcttcgcgg gtcatttgct
     3421 taccgtttgc atcttttccc caatatgcat ccgctgcttt ctgcgccgcc accatttgct
     3481 tatcaagcgc tttcacttcc ttttccagcg caggaatcga gttcttctgg ttattcaacg
     3541 cagactcaag ctgactaaag cgataattga aatcttcgct attcacgatc agatatttat
     3601 tattcgtttt gatatcgtcg accacccgcc cacgatcgct ggctgccgcc tgaaaagagt
     3661 aaaagttcac cgaccagcca gacgcgggcg ttcctttact ggttaacatc gccgagaatt
     3721 ttaccggttt atctttcgtc caggtctgtt cgagcagttc gtaatctgcc aactgaccga
     3781 cccaggtata gagatcgtca ctggaagaga catcgccttc cgctgaccag gtggcctgat
     3841 tacccttagc atcaagattt tttaaggtga tagcatcaag cttgagaatg ccgtgatact
     3901 gctttttgaa ttgatctttt aagatatttt cagttggcgg ttcgccatct gcccagacat
     3961 taccagtcat caacataagc agcgccgtgc caacaatcca ttgctttttc acccgaaaac
     4021 cctcgcgcgg aataatttat tccagtgatt atcatcaggg taatgaaaat tggcaatgga
     4081 cgtaagtctg aagtgaaaaa gccccggcac gataccgggg cgaggtgatt agtactgatt
     4141 gaagatctgc tggatctgct gcggatcttt ggtttgcgtc agagccagct gcagcaggac
     4201 gcgcgctttt tgcgggttca gcgtgccaga ggcgacgaag ccgtatttcg catcatccac
     4261 ttcagcatcc tgagtggtag cgcccgtcgg tacacgggaa gaacgcacta ctgcagtgcc
     4321 gtttttcgcg gcggttgcaa gggtgtcaaa tacggtttta tacaggttgc cgttacccac
     4381 accagcgctg acgatgccat catagcccgc atctaccagt gctttagccg gaagatcgga
     4441 tgcgttagcg tagttataaa cgatgccgac tttcggcagc tcattcagct tagagacatc
     4501 gaatggcgta tcgctggtgt gcttacgtgc cggggtacgt tggtagtcaa tcttaccgtt
     4561 gtgaatgtaa cccagaggac cgtagttaac agacttgaag gtcgctacgt cggtggtgtt
     4621 ggttttggtg acatcgcgac cgtccagtac ggtgtcgttc atcaccacca gtacgccacg
     4681 attagcggag gctttatcag ctgcggtcac taccgcgtta tacaggttga atggaccgtc
     4741 tgcgctcata gacgtggacg ggcgcattgc accgaccatc accaccggtt tgtcgcattt
     4801 caccgtcagg tcgaggaagt aagcggtttc ttccatcgtg tcggtaccgt gagtaatgac
     4861 gaagccgtcg gttttatcgc agtcggtgtt aatttttttc gccagtgtca gccagacatt
     4921 atcgttcatg tcctgggagc cgatattcac tacctgctcg cctttaacgt tcgcaatgtc
     4981 cttcagttgc ggcaccgcat taaccagatt ttctacgcca actttacccg ctgtgtagtt
     5041 agatttggtt gcggagtcac caccaccggc aatggtcccg ccggttgcta aaatggtgat
     5101 attgggtaat gccaatgctg caccactaaa acccataacc agtgcggcaa gtgccgtctt
     5161 tttgaaaaac tccatttcat tcctccagtt acgtgaacgc tacgcattat cccttagctt
     5221 tgtatgggaa atttgacgtt aaacaattta caacgtgaat atattttgga gatctacaaa
     5281 gttagaggca ggtaacaaaa cgaagaatta aacggcataa aaaagtatta tgccgtctta
     5341 aaatagagga ttattttaaa ttcccgacca gggctttgcg actatcttcc agagtcacaa
     5401 cgcggctaca aacatctttg ccaaactgct ggaaatcttt ttcctgcttt ttccattcgg
     5461 tttggattga ggattgcaga ccgcccaggc ttcccagcac attctgtaat gggttaccac
     5521 cgcttttcag caccgctttc gcgcccattt cattaatgct gtcctgtaaa attccgccca
     5581 ttgcctgatt cactaattgc tggccttcgg cgcgaacctg atcaatggct ttatagtgaa
     5641 acgtcaggcc atcgctgcgc gtttcgataa tgcggttcat ctgctctttc agctgcgcat
     5701 caagtttggt cagacggctg cgcattttgc tgctttcgcc catctcctga acgataattt
     5761 tatccagcgc aatacgggct ttctcgacgc ggcttttcgc gccttcatca atccacggca
     5821 gggtgctacg cagttcagcc tgataatcct tcgcctgctc gcgctgggcg gcgttcaggg
     5881 aatattgctt accgttatac atcacgttgc cgtctggcgt gatcaccaga ttgccgtttt
     5941 cgcccttcac ctgcacggtt tgcgggctga caatcacatc gtcacgcggc gtgacgctgc
     6001 actggtagtc ggcgtgagcg gtcattgccg tcactgaaag tgctgccgcc agcagcattt
     6061 tgcgcatcat agtcttccct caagaacaaa tcaggccagc atttgctggc cccagattga
     6121 taacaaagta catgtcatcc atgccggatg cggcgtaaac gccttatccg gcctactcga
     6181 ttgtgcatct ttccaggcct gataagcgta gcgcatcaga cagttttgca tttgtcatcg
     6241 cccgtatgtt ttcttagtcc caccaaacgt cgaaaagttc gctggtgcgt acctcgccca
     6301 gtttgcgctc ttccagccac ttacgcacaa tcgcctgatg ttcttcggtg catttgccga
     6361 tttcctgcat gcagatcaga ccttcccagg ccagataacc gctgccgtca aaggccagtt
     6421 tgttcggttc gataacctcg ttaataaagt catcaacggt tttatcaatc tgttcttccg
     6481 atgtaccttc cgggaatcgc catgccaccg aaaatcctaa ttcctggaat tcgtcgatgt
     6541 gcattttttt acgcagacga cggctacggt tctttgccat tatttcaccc tctcgaacat
     6601 taagtcccat actccgtgac caagacgatg accacgttgt tcaaatttcg tcaccggacg
     6661 tgatgccgga cgcggtacgt aatcattgct ctctgacagg tttttataac cgtcaataga
     6721 agacatcact tcaagcatat gttccgcata aggttcccag tcggtcgcca tatggaatac
     6781 gccccccagc tgcagtttgc tttttaccag ttcggcaaac ggcacctgaa cgatacggcg
     6841 tttattatgg cgcgctttgt gccacgggtc agggaaaaag agctgcacca tgcgcaatga
     6901 attgtcagga atcattttat gcagcacttc aaccgcatcg tgacacatca cgcgcaggtt
     6961 gcttaagccc tcttcatgcg cagaagccag gcacgcacca acgcccggtg aatgcacttc
     7021 aatgccgagg aagtcctgct cggggcgatc tttagccatt gccaccagcg acgcccccat
     7081 gccgaaacca atctcaagcg tcaccggcgc ttcacggcca aaaagcgcag ggaaatccag
     7141 catatcttcg ctgaactcaa cgcccatcac cggccagtag ttttccagcg catgttcctg
     7201 gcctttggtc agtcgcccct ggcggcgcac aaaactacgg atacggcgca gtgggcggcc
     7261 gttttcatca aattccggtg agatgacgtc gtttttcata aagatttagt cgcttgtgaa
     7321 agtgttctga aaacgggcat tatccaaagt tagttgccgg atgcaagcat gataaggccg
     7381 tggctgcgga aagttccggt ttacaccctg ccgtcgctgt gctgcaatct tgcccccaac
     7441 aacagtgaat tcggtgacca tgcaagcgtc gcaattttca gcccaggttc tggactggta
     7501 cgataaatac gggcggaaaa cgctgccctg gcaaattgac aagacgccct acaaagtatg
     7561 gctctcagaa gtgatgttgc aacaaactca ggttgcgacc gttatcccct attttgaacg
     7621 ctttatggcg cgcttcccaa cggtgaccga tctcgccaat gcgccgctcg acgaagttct
     7681 ccacttgtgg accgggcttg gctattacgc ccgcgcgcgc aatctgcata aagcggcaca
     7741 gcaagtggcg accttacacg gcggtaaatt cccggaaacc tttgaagaag tcgcggcgtt
     7801 accgggcgtc gggcgttcca ccgcaggcgc gattctctcg ctttctctgg gtaagcactt
     7861 tccgattctc gacggtaacg tcaaacgggt gctggcgcgc tgctatgctg taagcggctg
     7921 gcccgggaaa aaagaggtcg agaataaatt atggagtttg agcgagcagg tgacgcccgc
     7981 tgtcggcgtg gaacggttta atcaggcgat gatggattta ggcgcgatga tttgtacgcg
     8041 ctcgaagccg aaatgttcgc tctgtccgct acaaaacgga tgtattgcca ccgccaacaa
     8101 tagctggtcg ctttatccgg gcaaaaaacc gaaacagacg ctgccggagc gcaccggcta
     8161 ctttctgctg ttacagcacg aagatgaagt attgctggcg cagcgcccgc cgagtggatt
     8221 gtggggcggt ttatactgtt tcccacagtt tgccgacgaa gaaagtttgc ggcagtggct
     8281 ggcgcaacgg cagattgctg ccgataacct gacgcaactg accgcgtttc ggcatacctt
     8341 cagccatttc cacttagata ttgtgcctat gtggcttccc gtgtcgtcat tcaccggctg
     8401 catggatgaa gacaatgcgc tctggtataa cttagcgcaa ccgccgtcag ttggcctggc
     8461 ggctcccgtg gagcgtttgt tacagcagtt acgcactggc gcgccggttt agcgcgtgag
     8521 tcgataaaga ggatgattta tgagcagaac gattttttgt actttcctgc aacgtgaagc
     8581 agaaggtcag gattttcagc tgtaccccgg cgagctggga aaacgcatct ataacgagat
     8641 ctccaaagaa gcctgggcgc agtggcagca caaacaaacc atgctgatta atgaaaagaa
     8701 actcaacatg atgaatgccg agcaccgcaa gctgctggag caggagatgg tcaacttcct
     8761 gttcgagggt aaagaggtgc atatcgaggg ctatacgccg gaagataaaa aataaaaaca
     8821 gtgccggagc acgcctccgg caacttgcat aaaaacaaac acaacacgca cccggaatga
     8881 tgaaaaaata tctcgcgctg gctttgattg cgccgttgct catctcctgt tcgacgacca
     8941 aaaaaggcga tacctataac gaagcctggg tcaaagatac caacggtttt gatattctga
     9001 tggggcaatt tgcccacaat attgagaaca tctggggctt caaagaggtg gtgatcgccg
     9061 gtcctaagga ctacgtgaaa tacaccgatc aatatcagac ccgcagccac atcaacttcg
     9121 atgacggtac gattactatc gaaaccatcg ccgggacaga acctgccgcg catttgcgcc
     9181 gggcaataat caaaacgttg ctgatggggg acgatccgag ttcggtcgat ctctattccg
     9241 acgttgatga tattacgatt tcgaaagaac ctttccttta cggtcaggtg gtggacaaca
     9301 ccgggcagcc gattcgctgg gaaggtcgcg cgagcaactt cgcggattat ctgctgaaaa
     9361 accgtctgaa gagccgcagc aacgggctgc gtatcatcta cagcgtcacc attaacatgg
     9421 tgccgaacca ccttgataaa cgtgcgcaca aatatctcgg tatggtccgc caggcgtcac
     9481 gcaaatatgg cgttgatgag tcgctgattc tggcaattat gcagactgaa tcttccttta
     9541 acccgtatgc ggtcagccgt tccgatgcgc tgggattaat gcaggtggta caacataccg
     9601 ccgggaaaga tgtgttccgc tcgcagggga aatccggcac gccgagccgc agtttcttgt
     9661 ttgatcctgc cagcaatatt gataccggca ccgcgtatct ggcgatgctg aacaacgttt
     9721 atctcggcgg aattgataac ccaacatcgc ggcgttatgc cgtcatcacc gcctataacg
     9781 gcggcgcagg cagcgtgcta cgagtctttt cgaatgataa gattcaggct gccaatatta
     9841 ttaacaccat gacgccgggc gatgtttatc agacgctgac gacccgccat ccctctgcgg
     9901 aatctcgccg ttatctttat aaagtgaata ccgcgcaaaa atcttaccgc cgccgataat
     9961 tccactaacc gcccctgacg atgctcaggg gtaaaaatgt tatccacatc acaatttcgt
    10021 tttgcaaatt gggaatgttt gcaattattt gccacaggta acaaaaaacc agtccgcgaa
    10081 gttgatagaa tcgcatcatc tcgcacggtc aaatgtgctt tttcaaacac tcatccgcat
    10141 cacgatgtga ggaaattaac atgaatctta agctgcagct gaaaatcctc tcttttctgc
    10201 agttctgtct gtggggaagt tggctgacga ccctcggctc ctatatgttt gttaccctga
    10261 agtttgacgg tgcttctatc ggcgcagttt atagctcact gggtatcgcc gcggtcttta
    10321 tgcctgcgct gctggggatt gtggccgaca aatggttaag tgcgaaatgg gtctatgcca
    10381 tttgccacac cattggcgct atcacgctgt tcatggcggc agaggtcact acgccggagg
    10441 cgatgttcct tgtgatattg attaactcgt ttgcttatat gccaacgctt gggttaatca
    10501 acaccatctc ttactatcgc ctgcaaaatg ccgggatgga tatcgttact gacttcccgc
    10561 caatccgtat ctggggcacc atcggcttta tcatggcaat gtgggtggtg agcctgtccg
    10621 gcttcgaatt aagccacatg cagctgtata ttggcgcagc tctttccgcc gttctggttc
    10681 tgtttaccct gactctgccg catattccgg ttgctaaaca gcaagcgaat cagagctgga
    10741 caaccctgct gggcctcgat gcattcgcgc tgtttaaaaa caagcgtatg gcaatcttct
    10801 tcatcttctc aatgctgctg ggcgcggaac tgcagattac caacatgttc ggtaatacct
    10861 tcctgcacag cttcgacaaa gatccgatgt ttgccagcag cttcatcgtg cagcatgcgt
    10921 caatcatcat gtcgatttcg cagatctctg aaaccctgtt cattctgacc atcccgttct
    10981 tcttaagccg ctacggtatt aagaacgtaa tgatgatcag tattgtagcg tggatcctgc
    11041 gttttgctct gtttgcttac ggcgacccga ctccgttcgg tactgtactg ctggtactgt
    11101 cgatgatcgt ttacggttgc gcattcgact tcttcaacat ctctggttcg gtgtttgtcg
    11161 aaaaagaagt tagtccggca attcgcgcca gcgcgcaggg gatgttcctg atgatgacca
    11221 acggcttcgg ctgtatcctc ggcggcatcg tgagcggtaa agtggttgag atgtacaccc
    11281 aaaacggcat taccgactgg cagaccgtat ggctgatttt cgcaggttac tccgtggttc
    11341 tggccttcgc gttcatggcg atgttcaaat ataaacacgt tcgtgtcccg acaggtacac
    11401 agacggttag ccactaatta cgcaaagaaa aacgggtcgc cagaaggtga cccgtttttt
    11461 tattcttact tcaacacata accgtacaac cgtttcatgc cattcgcatc ggtttcgcta
    11521 tagacacctt gcaactccgg cgaaaatccc ggcaacaaat tcacgccttc ttccagcgca
    11581 aggaaataac gttgaaccgc cccaccccag acttccccgg gtaccacgca aagcacgcca
    11641 ggtggataag gcaacgcccc ttctgccgca attcgccctt cggcatcacg aatccgcacc
    11701 aactccactt caccgcgaat ataagcgcta tgcgcatcct ggggattcat caccactgac
    11761 gggaaactct gctggcggaa catcgctttt tgtaggtctt tgacgtcgaa actgacatac
    11821 agatcgtgca tctcctgaca caactgacgc agggtgtagt cgcgatagcg caccggatat
    11881 ttgttataaa cgctcggcaa cacctcagcc agcggcgagt catcctcaat atgctgttca
    11941 aactgcgcca gcatcgccac cagttgtgcc agcttctcgt ggctttccgc cggagttaat
    12001 aagaacagaa tggagttgag atcgcacttc tccggcacaa tgccgttctc acgcaggtag
    12061 tgcgccagaa tcgtcgctgg aacgcaaaaa tcgctatatt cgccggtttc ggcatcgata
    12121 cccggcgtgg tgagtaacag cttgcacgga tcaacaaaat actgatccgc ggcatatcct
    12181 tcaaagccgt gccacttcgc ccccggctca aaactgaaaa aacggcggtc gctggctaac
    12241 actgacgtcg gataatcctg ccacaatttg ccatcaacaa cgggcgggat aaacgggcgg
    12301 aacagcttac agcgcgcaag aatcgccttc cgggcttcaa tccccaacgc aacgcactcc
    12361 gcccacagcc gacgcccact ctccccttca tgaattttgg cgttaacgtc cagcgcggcg
    12421 aacagcggat agaacgggct ggtagaagca tggagcataa aggcattatt caaccgctta
    12481 tgcgggcaaa aacgcgcctg tccacggata tggttatctt ttttatggat ctgcgacgtc
    12541 tgtgagaatc ccgcctgctg tttgtgcacc gactgggtca caaagatccc cggatcgttt
    12601 tcgttaagtt ctaacagcag cggcgagcta tccgccatca tcgggataaa ctgttcgtaa
    12661 ccgacccacg cggaatcaaa cagaatgtaa tcacacagat gcccaacggt atcgatcacc
    12721 tgacgggcgt tatagacagt gccgtcatag gttcccagct gaataatcgc caggcgaaac
    12781 gggcgcggca ggtcggcttt ttctggcgca acgtcgcgaa tttgctggcg cagatactct
    12841 tcattaaaac agtgcgcatc aataccgcca atgaaaccaa acgggttacg tgaagcttcc
    12901 agatagaccg gcgtcgcccc cgcctgaatc aacgcgccgt gatgattcga cttatggttg
    12961 ttacggtcga agagcaccaa atcgccacgc gttaacagcg cattcgtcac cactttattc
    13021 gctgccgatg tgccgttcag cacaaaatag gttttatcgg catgaaagac tttggctgcg
    13081 aatttctgcg catctttcgc cgatccttca tgaatcagca gatcgcccaa ttttacgtca
    13141 gcgttacaca tatcggcgcg aaagatgttc tcgccaaaga aatcgtaaaa atggcgtccg
    13201 gcaggatgct ttttaaagaa cgcaccatgt tgatgtccag ggcaagcaaa ggtgctatta
    13261 cccatctcaa cgtactgcgt cagcgtgtca taaaacggtg gcagcaaatt ctcttcatac
    13321 tgacaggctg cggattccag ctccagccac tgctgctcgt tgccgttgat taccgccgta
    13381 acgcccgcag gtaattcaac aacatgttcg gaatacaaaa acaccggtag atgaaaaccg
    13441 gtgcgcttaa gcaacgcaag aatgccactg cgactatccg cagcggtaat gacgactgcc
    13501 gcgacgtccg taaaatcagt atctgccaac gccaccacgc ggcgatgagt agaaagtcgg
    13561 gataccagtt cactactggc ggcaatattc attgatttca taagcgcaaa acccgtttcg
    13621 gggaagtaag aataccggac aaggtgaaaa accctgcccc acgagatatg ggtcaaactg
    13681 gtcaccagct ccgaccgcca gacatcagta aaagcagaaa cgctctgatt ttactgttgt
    13741 cctgcagtga gcgtgcgtta acttcaccgc atgagcagaa aaagggagga gtttcgcgcg
    13801 aaacggcgat aagcgagaga atgtaaggag gtggcgtgca tcgggcaaac tccgtacaga
    13861 gaggagaaaa ttcgcgcaat catggcacct ttcgctaaag cgtgcaagcc aggactttgc
    13921 gaccagacaa gccatcaaca aggcaaaaag ttcataataa gaaaatcaaa caatacagtt
    13981 aacaggaact cttgtggtca tcggcccttt tatcaacgct agtgcagtct tactgggtgg
    14041 cgttctcggc gcactgctca gccaacgctt accggaacgt atccgcgtct ccatgacatc
    14101 aatttttggt ctggcatcgc tggggattgg tattttactg gtggtgaaat gcgccaacct
    14161 tccggcgatg gttttagcaa ccttactcgg tgcattaatc ggcgaaattt gtctgctgga
    14221 aaaaggcgtc aataccgcgg tcaccaaagc acaaaatctg tttcgccact cacgtaagaa
    14281 gccagcgcat gaatctttta ttcagaatta tgtcgcgatt attgtcctgt tttgcgccag
    14341 cggcaccggg atcttcgggg cgatgaacga agggatgacc ggcgatccga gtattttaat
    14401 cgccaagtca tttcttgatt tctttacggc gatgatcttc gcctgctcgc tgggtattgc
    14461 ggtatcggtg attagtatcc cattactgat catccagtta acgctggcgt gggctgccgc
    14521 gctgatatta ccgctgacca caccgtcgat gatggcagac ttcagcgccg taggcggttt
    14581 attgctgctg gcaaccggat tacgcatctg tggcattaaa atgttcccgg tggtcaacat
    14641 gcttccagca ctccttctgg caatgcccct ttccgccgcc tggaccgcct ggtttgcctg
    14701 acaatgcgtg cattagcggc aaagtgatga tagattgtgc agtctgcagt aaattgaaga
    14761 aatttgattg acgagacgag gcgaatcagg tttaatgcgc cccgttgccc ggatagctca
    14821 gtcggtagag caggggattg aaaatccccg tgtccttggt tcgattccga gtccgggcac
    14881 caagttcata tcaacggacc tccacggagg tccgtttttc gtttcagaac gccacaattt
    14941 aagcgttctg cctccaaatc aattctaccg aactcaacca gattcccccc acatcaaccc
    15001 cattgtgtgg gtataattgc gggtataccc cagttcgaca gaatttgtac cctctttaac
    15061 gcattcagaa ggctgaacta tggcactgac tgacgcaaaa atccgggctg caaagcccac
    15121 tgacaaggct tataaactca ctgacggggc tggcatgttc ctgctggtac atcctaatgg
    15181 ttcccgttac tggcgtctcc gttatcgtat tctgggtaag gagaaaaccc tggcacttgg
    15241 tgtgtatccg gaagtttctc tctccgaagc tcgtacaaaa cgggatgagg cccgaaaact
    15301 gatttcggag ggggttgacc cttgcgaaca gaaaagagct aaaaaagtag tccctgattt
    15361 acagctctct tttgaacata ttgcacgacg ctggcatgcc agtaataaac aatgggcaca
    15421 atcacacagc gataaagtac tcaaaagcct cgagactcac gttttcccct ttatcggcaa
    15481 ccgggatatc acaacactca gtaccccgga tctgcttatc cctgttcgtg ctgcagaagt
    15541 taaacaaatt tatgaaatcg ccagtcgtct gcagcaaaga atatctgctg taatgcgtta
    15601 tgccgtacag tctggcatca tcagatataa cccggctctg gatatggctg gtgcattgac
    15661 cacggtaaaa cgccagcatc gccctgctct ggatctttca cgcctgcctg aacttctgtc
    15721 gcgtattgac ggttataaag gccagcctgt cacccgactg gcagttatgc tgaatttact
    15781 ggtttttatt cgttccagtg aactcagata tgcccgctgg tctgaaattg atattgacaa
    15841 tgccatgtgg actattccag ccgaacgcga acctctgcca ggcgtaaaat tttcataccg
    15901 gggctccaag atgcgaacac cacatcttgt gccactcagc caacaggctg tagccatact
    15961 ggcagaactt cagacatggg caggtgaaaa tggtctgatc tttacgggag cacacgaccc
    16021 acgtaaacca atcagtgaaa ataccgtaaa taaggccctc agagtgatgg ggtatgacac
    16081 aaccaaggaa gtctgtggcc atggattccg ggcgatggcg tgcagtgcat tgattgaatc
    16141 cggcttatgg tcaagagatg cggttgaacg tcagatgagt catcaggagc gcaacggagt
    16201 acgtgccgcg tatattcata aagcagaaca tctggaagaa cgtcggctta tgctgcaatg
    16261 gtgggcagat tttctggatg caaacaggga ggagtgtatc agtccgtttg aatatgcaaa
    16321 agttaacaat ccactgaaaa ggtgactgaa cgccatttac acacatgctc ttcctcttaa
    16381 ggggaagagc acccgcctca atcaatttct tttttatgcc ggcggaacca tcagccaggc
    16441 tgaggttctc attcaataac tcaacgtctt accacagaac agaattcact gtattatcat
    16501 gggcaacgtt aatcccggac aataaaagga caccgatgcc aggcatcaca atttttatag
    16561 cctgcaggtt atggtccatc cttttttaca ggttcagaat atgactggca aattacgctt
    16621 tgaagtgaac gataaccagg gctgtttcat atttccggaa acctggtttg gctcgttgct
    16681 ggatgagttt gaagaactga ttgatgcata cgatgcggat gaaatcagcg aaacaagtta
    16741 catcaacaaa ctcaggcgcc ttgcccgaca ggagaatgac tttattgatg tacacgctca
    16801 cttggcatac gtatttctgg agcagaatgc cccgcgtaag gcgctgaatg ctgcccttaa
    16861 agggctggca attggcaatc gtcttatccc tgaaggtttc agtggcagaa tcatctggat
    16921 acatcctgac aaccgacctt ttcttcgtgc cctctatgcg gcaatcctgg caaatgcaca
    16981 tttacagcgt catcaggatg ccatcatgct cattgaaaaa atactggatt acaatcccga
    17041 agataatcat ggtgcacgct ggctactggg accggaactg ttacgcactg gcgctcatga
    17101 acaggcacgc catatactgc aggaacatgc agatgaattt tcaccttact ggtatgaact
    17161 cggccttctg cattttctta atggggaact ggtaaaagct gcaactgcat ttcgtcgcgg
    17221 ctttgcggca aatacttaca tcgcagaaat actgtgcgga aatctgcatc catttccact
    17281 tgctgtatgg cataactttt caggcggtcc ggatactgcc gaagattatt atgccacata
    17341 ccacccgtta tgggggcaat atccggaagc attgctgttt gtaaactggc tctataatca
    17401 ttcctctgtg ctgcatgaac gggcagaaat cattaaatgc gccgaaatgc tgatgcagga
    17461 agatgatttc gaaatatgcg aaagtatttt aagacagcag gagaagttgc gtgaaagaat
    17521 tgatgagacg ctttctgaga aaattgtaca gaaatgcaga aatatgaatg gtgaatatat
    17581 ctggccctgg atattgccgt tttcagcggc aggcatgaaa catactggca tacagtatca
    17641 gtagatattg cattagtgta tcctgcacac aagtaataat ttattcacca ataataacac
    17701 tgttaatgcc cccttcccct ggttgtcagc caggggttat cttctgaata tttcttttga
    17761 aaaggataac acagtaaatt atttttatga attatcccat ggactcatta acaccctttc
    17821 atgatgcttt attgtcaaac acgttatggc tgacatcaaa aaaaaccgga tttcctctgc
    17881 cagcgggtaa tcaccttccc ggtgttttcg gttggtctgg ttactcctgt ctggttatta
    17941 gcaagataat tgctataaac agtggaaaac tcatcgtaca taatctggtg atgaacatta
    18001 cgcttatttt cccttgaccg gaagaatcag aggctgtggt ttcagactgt ctgccagtac
    18061 attcctctct ccgttaaaaa ccataacggg ttcattatct tcgtctgtca gca
//
LOCUS       DACADA010000088        17933 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.089, whole genome shotgun sequence.
ACCESSION   DACADA010000088 DACADA010000000
VERSION     DACADA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.089"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            18..1118
                     /gene="chaA"
                     /locus_tag="ISB17_002956"
     CDS             18..1118
                     /gene="chaA"
                     /locus_tag="ISB17_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAP1980859.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1523..1630
                     /locus_tag="ISB17_002957"
     CDS             1523..1630
                     /locus_tag="ISB17_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAP1980860.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(1779..2633)
                     /gene="kdsA"
                     /locus_tag="ISB17_002958"
     CDS             complement(1779..2633)
                     /gene="kdsA"
                     /locus_tag="ISB17_002958"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAP1980861.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2669..3478)
                     /locus_tag="ISB17_002959"
     CDS             complement(2669..3478)
                     /locus_tag="ISB17_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAP1980862.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(3482..3874)
                     /locus_tag="ISB17_002960"
     CDS             complement(3482..3874)
                     /locus_tag="ISB17_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAP1980863.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(3871..4704)
                     /gene="prmC"
                     /locus_tag="ISB17_002961"
     CDS             complement(3871..4704)
                     /gene="prmC"
                     /locus_tag="ISB17_002961"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP1980864.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(4704..5786)
                     /gene="prfA"
                     /locus_tag="ISB17_002962"
     CDS             complement(4704..5786)
                     /gene="prfA"
                     /locus_tag="ISB17_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP1980865.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(5828..7084)
                     /gene="hemA"
                     /locus_tag="ISB17_002963"
     CDS             complement(5828..7084)
                     /gene="hemA"
                     /locus_tag="ISB17_002963"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAP1980866.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7298..7921
                     /gene="lolB"
                     /locus_tag="ISB17_002964"
     CDS             7298..7921
                     /gene="lolB"
                     /locus_tag="ISB17_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAP1980867.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7921..8772
                     /gene="ispE"
                     /locus_tag="ISB17_002965"
     CDS             7921..8772
                     /gene="ispE"
                     /locus_tag="ISB17_002965"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP1980868.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFRFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            8923..9870
                     /locus_tag="ISB17_002966"
     CDS             8923..9870
                     /locus_tag="ISB17_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAP1980869.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            9995..11674
                     /gene="dauA"
                     /locus_tag="ISB17_002967"
     CDS             9995..11674
                     /gene="dauA"
                     /locus_tag="ISB17_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAP1980870.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(11729..12007)
                     /gene="ychH"
                     /locus_tag="ISB17_002968"
     CDS             complement(11729..12007)
                     /gene="ychH"
                     /locus_tag="ISB17_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAP1980871.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            12285..12869
                     /gene="pth"
                     /locus_tag="ISB17_002969"
     CDS             12285..12869
                     /gene="pth"
                     /locus_tag="ISB17_002969"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP1980872.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            12986..14077
                     /gene="ychF"
                     /locus_tag="ISB17_002970"
     CDS             12986..14077
                     /gene="ychF"
                     /locus_tag="ISB17_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP1980873.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEATNFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            14292..15467
                     /locus_tag="ISB17_002971"
     CDS             14292..15467
                     /locus_tag="ISB17_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAP1980874.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            15578..15862
                     /locus_tag="ISB17_002972"
     CDS             15578..15862
                     /locus_tag="ISB17_002972"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1980875.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            15859..16713
                     /locus_tag="ISB17_002973"
     CDS             15859..16713
                     /locus_tag="ISB17_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1980876.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(17336..17722)
                     /locus_tag="ISB17_002974"
                     /pseudo
     CDS             complement(17336..17722)
                     /locus_tag="ISB17_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         4378 a         4567 c         4543 g         4445 t
ORIGIN      
        1 gcaaagggat aaccataatg tcaaatgctc aagaggcggt aaaaacccgc cacaaggaga
       61 cttcgcttat tttcccggtt ctggcgctgg tagtgctgtt cctgtgggga agcagccaga
      121 cactaccagt ggtcattgcc atcaatcttc ttgcgcttat tggtatttta agtagcgcct
      181 ttagtgttgt ccgtcatgcg gacgtattag cccatcgcct gggagaacct tacggttcgc
      241 ttattcttag cctttcagtg gttattcttg aagtcagttt gatttcagct ttaatggcaa
      301 ccggcgatgc cgcgccaacg ctaatgcgtg atacgctcta ttcaatcatt atgattgtta
      361 caggtgggct ggttggcttt tcattattgt tgggcggacg taagtttgcc acccaatata
      421 tgaatctgtt tggtatcaag cagtatttaa ttgcgctgtt ccccctggcg ataatcgtac
      481 tggtatttcc aatggctctg cctgcggcga atttttcaac aggtcaggcg ttactggtag
      541 cattaatttc tgcggcaatg tatggcgtat ttttgctgat ccagaccaaa acgcatcaaa
      601 gtttgtttgt ctacgagcac gaagatgaca gtgatgatga cgacccgcac cacggtaaac
      661 cgtctgccca tagcagcctg tggcatgcca tctggttgat tgtccatctg attgccgtta
      721 ttgcagtcac caaaatgaac gccagcccgc tggagacatt gctcgacagc atgaatgcgc
      781 ctgtcgcctt tactggcttc ctggtggctc tgttgattct gtcgccggaa ggtttaggtg
      841 cgttaaaagc agtgttgaat aaccaggtcc agcgcgcgat gaatctgttc tttggttcag
      901 tgttggcaac catttcgctt accgtacctg tcgtcacgct aattgccttt atgacgggta
      961 acgaattgca gtttgcactt ggtgcgccag aaatggtggt gatggtggcc tctttagtac
     1021 tgtgccatat ctccttctcc accggacgaa ctaacgtgct caatggcgca gcgcatctgg
     1081 cactgtttgc cgcctatttg atgacgatat ttgcctgaca gttatatctc ctccgatgac
     1141 ggaaatcggg ggagataata taaattcctg cttattatat ttcttcatac tagctggaca
     1201 ggttgttaat cagaattgca agcctgtacc acaacctgca cgaaatgact gcaacagctg
     1261 ttgaaaaacc ggaatgtact ctttattgca aacatttcag ggatgaagtg aagatgaatt
     1321 gcgacgtagt atccacacca agccggcgtt atccggtgag gcgcaatgtt gcgggggctt
     1381 tatccctggt ggcattggtt gctggaaaga gaaaaccccc gcacgttgag aggtacaacc
     1441 tgacaacaga acgggggcta atcttgaacc agaacaattc aagaatagcc gcaaaatgtt
     1501 gtcattacaa caaggcggct atatgacgct cgcgcagttt gccatgattt tctggcacaa
     1561 cctggcggca ccgatcctgg cggggattat taccgcagtg attgtcagct ggtggcgtaa
     1621 ccggaagtaa ggtgaaaacg ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg
     1681 gccgcagggg taacagcctg cggccttttt tgtatccggc acccatgaaa aaagtcttaa
     1741 cgcagaacgc taagacttta tttttcaggc aaaaaagatt acttgctggt atccagttct
     1801 tcgaaacctt tcaccagatc atcaatcgct ttcatttgct tgaggaacgg ttccagttta
     1861 gccagcggca gcgcggatgg accatcacat ttcgcatgtt ccggatccgg atgcgcttca
     1921 ataaacagcc ccgccagacc taccgccata ccggctcttg ccagctcagc cacctgagca
     1981 cggcgaccgc cggaagctgc gccaaatgga tcgcgacatt gcagtgcgtg ggtaacgtcg
     2041 aaaatcaccg gcgaattacc agacactttc ttcatgatgc tgaagcccag catatcgaca
     2101 accaggttgt catagccgaa gttagcaccg cgatcgcaaa gaatcacttt ttcgttgccg
     2161 ccttctttga atttatcaac gatattaccc atctgtcccg ggctgacaaa ctgtggtttc
     2221 ttgacgttaa ttaccgcacc ggttttcgcc atggcttcaa ccaggtcagt ctggcgagca
     2281 agaaacgccg gcaactgaat cacatccacg acatcagcaa cgggctgtgc ctgacttggt
     2341 tcgtgaacgt cggtgataat tttcacgcca aaagtctgct tcaactcctg gaagattttc
     2401 atcccttctt ccaggcccgg tccacgataa gagtggatgg aggagcggtt ggctttgtca
     2461 aaagaggctt tgaacacgta agggataccc agtttctggg tcacggttac gtagtgctcg
     2521 caaatgcgca tcgccagatc gcgagattcc agcacgttca taccgccaaa cagtacgaac
     2581 ggcaggtcat ttgctacgtt gatgtcacca atgctaacca ctttttgttt cataggatcg
     2641 ccttaatctt gagtaaaatg tcgattaatt aatgcagcac aatatgttta tgcgcgatgt
     2701 tatttatttg cgcacggatc atttcgctga tcgggtcttc cggacactgt tcaacgaaat
     2761 aacttaaatc gttcaacgca acgtgttcgc aatccagttg cgcataaatc aacccgcgat
     2821 cgcgaatttc atagggatct tccgggttga attgtaataa agcttcgctg gtgcgtaacg
     2881 ccagctccat ctgattttct tccatcaacg aagctttgag tgtatccagc aatttgcgga
     2941 ttacctcaat gttatcagct tcatcaaggt cttcataaaa cagttccgcc gacgggctga
     3001 tatttccctt taaccacaca tccagcatat gttcgcttaa cgattcaccg ttaaaaggat
     3061 taatcagcca aatttcgcca tccggacatt caatgcgcaa tatcagctgc gtagggaaaa
     3121 tcaccggcag cagcggcaaa tcgagacgat tcgcgaccca taataaaacc gcacctaatg
     3181 ataccgcact gccctgtcga ttctttaaca cctggtccag ccataatgca tcggaaagac
     3241 gataaacgcc gcgtgaggct ttaaatcccc agtcgccgta aaacagcgcg atcagttttt
     3301 ccaactgctc ttctaaaggc agaagctggc taatttcttc cttcgccaga ctaacgagac
     3361 gctccagttc gtcgtaaaca tcttgcgagg gaaaatcgcg gcggattgct tcgcaagcca
     3421 ggatcatgcc ttcgcacaat ggcgctttat taaattcgaa atcagctaac gatctcatga
     3481 cttaccccag taacggtact tttgtggtgg cgagtttaat gatgatgtac agcaccacca
     3541 acgccagcgg gaaggcaata atgcgcgcct gctgactgtg catccgacga taatcgagtg
     3601 caataaaacc caaaacgata taaatgataa ctccaaacag cttttcagtc agccactgtg
     3661 cctgcccgga aaatggctgg atgtgcgctt tagccatcaa cgcaatgccg cttaacaata
     3721 acagcgtatc gacaaccggc ggcacaatgc gcgtccagcg agcaaatgcc tgtggatgct
     3781 tctgataacg tagccagaaa cgtaaggtca atagcccaac agaaagcgcg atactaatga
     3841 gatgaacgct aagcaacgta gaaaaacttg tcattgataa tagcggccga gcgttacgcg
     3901 ctcgttatcc ccatagtcac ggcaggtttc gacgtcatga taccccgcga ggataaatgc
     3961 ctgtcgcacc gcttcaccct gctgccagcc atgctccaga agcagaaagc cgccggatac
     4021 cagcgcgtta cgcgactgtt cgatgatatg cacgatgtct gccattccac tgtctgccgc
     4081 aaccagcgca gtgagcggct caaagcggac atcgccttgt tgaagatgtg ggtcctgctc
     4141 gtcaatatac ggcggattgc tgacaatcat cgcaaactgc tgcccggcta gcgcgctaaa
     4201 ccagtcgctt tgcagaatgt ggatattttt gatagccaga tgctgggcat tacgttgtgc
     4261 cagggcgaca gcatcgggca tacgatctac agcgataatt tcgcagtccg ggcgctcgct
     4321 ggcaagcgca agcgcaatcg ccccggtacc cgttccgaga tcgagaatac ggcaaggttg
     4381 ttcaggcaac cgcgccagtg cctgctccac cagacactcc gtatccgggc gcggaattaa
     4441 ggtcgctggc gaaacaaata acggcaacga ccagaattct cgcaccccgg ttaaatgagc
     4501 aatgggttca ccatcgcgac gacgcgtcag tagcgcatca agttgctgac attgttcgtc
     4561 agtcagctgc gtttcaccaa aggcgagaat aaaagtacgc cctttgccgg taacatgctc
     4621 cagcaggatt tcagcatcac gccgcgggct ttcgctcgcc tgaagttggc ttattgcttc
     4681 acgtaaccag tgttgatatt ccattattcc tgctcggaca acgccgccag ttggtcggcc
     4741 tgatgttcct ggataatcgg ttcaatcagc atatccagct taccttccat cacttcatcc
     4801 aggcggtaga gcgtcaggtt gatgcggtga tcggtaacgc gcccctgcgg gaagttgtaa
     4861 gtacggttac ggtcgctgcg atcgccactc cccagcaggt tacgacgggt agacgcttcg
     4921 gcctgttgcc gttttgccat ttcagcagcg tggatgcgag caccgagtac agaaagtgct
     4981 ttagctttgt ttttatgttg tgaacgttcg tcctgacatt caacaacaat cccggtcggc
     5041 aagtgagtaa tacgaattgc cgaatcggtg gtgttaacgt gctgaccacc cgcccctgac
     5101 gagcggaaag tatctatgcg caaatctgct gggttgatgt ccggcagttc tgcatcaggc
     5161 agttctggca ttaccgcaac ggtacaagca gaagtatgaa tacgaccctg cgattccgta
     5221 gcaggaacac gctgcacgcg atgaccgcca gattcgaatt tcaaacgacc atatacacca
     5281 tcaccgctaa ttttggcgat gatctcttta taaccaccat gttcaccctc gctggcgctc
     5341 atgatttcta cacgccagcg gcgggcttcg gcgtaacggc tgtacatacg gaacagatcg
     5401 ccagcgaaca gcgccgcttc gtcgccgccg gttccggcac ggacttcgag gaaggcgtta
     5461 cgttcgtcat caggatcttt tggtagtaac agaacctgta attgctgttc cagttgctcg
     5521 cttttttctt tagcttcgcg cagttcatcc tgcgccatct cacgcatttc aggatcgtca
     5581 agcatcatct gtgcggtttc gatatcttcc tgaacctgtt gccagtcggt aaaacagcgc
     5641 gaaacatcac ttaactgcgc atattcgcgt gataatgcgc gaaaacgttc ctggtcggcg
     5701 atagtttgcg cgtcacccag caacgcctga acttcttcat ggcgttcatg cagggcttcc
     5761 agtttggcaa cgatagaagg cttcataggc gtaaatgcac cctgtaaaaa aagaaaatga
     5821 tgtactgcta ctccagcccg aggctgtcgc gcagaatatt caggcgttcg ttatccccgt
     5881 cacgggcggc ctgttgaagt gattttgttg gcgcatggat caagcggtta gtcagtttcc
     5941 atgccagatc ctgcataatg gcttgtgcgt cgccgccctg ctcaagggcc gctaacgctt
     6001 tggcggttaa ctcatcgcga acgtgctctg cctggctgcg atactcgcga atagtttcgc
     6061 tggcgctttg tgcacgcagc cacgccataa attcgctggt ttcctgagcg acaatagttt
     6121 ccgcctcaac cgctgcggct ttacgctgcg ccaggttgtg cgaaatgatg ctttgcagat
     6181 cgtccacgct ataaagataa gcattcgcca gtttgccaac ttccggctca acatcgcgcg
     6241 gaacagcaat atccaccaac agcattggtt ggttgcgacg gctttttaat gcgcgctcca
     6301 ccatgccttt cccgataatc ggtaacgggc tggcggtgga actgatgatg atatcggctt
     6361 cgcgcagacg ttcgtcgata tcactcaggg caataatttc cgcgccgact tcatctgcca
     6421 gaatttgggc acgttcgcga gtgcggttgg cgataatcat cttctgtact ttgtgttcgc
     6481 gcagatgacg cgccaccagc tcgatggttt cgcccgcacc caccagcagt actgtcaccg
     6541 tagagagcga ttcaaagatc tgccgcgcca gcgtacaagc cgcaaaagcg acagacacag
     6601 cactggcacc gatatctgtt tcagtgcgaa cgcgtttcgc cacagagaaa gatttctgaa
     6661 acatgcgttc tagttcgctg gccttcatat gacctttttg cgaatcggca aacgcttttt
     6721 taacctgacc gaggatctgc ggctcgccaa ggacgagaga atcaagccca ctggcaacgc
     6781 gcattaaatg actgaccgca tcgttatcct gatgccagta gaggctatta cgcagatcgt
     6841 cttcgttgag attatggtaa tcacacaacc agcgaatgag cgcctcttgc aggtcatccc
     6901 gttcttcaac gctgagataa agttctgtgc ggttacacgt cgacagcacc acgccgccct
     6961 gcaccatcgg ctgcgcgagc agactgtcaa gcgcctgatc gagcttatcc ggcgaaaacg
     7021 atacacgttc tcgcagcgat acaggtgccg ttttatggtt gataccgagt gctaaaaggg
     7081 tcatgtctgc gggaaataat accaacgttg atagggttag tctgcttgca tcatacagga
     7141 tgcgtaagat caataaaaga gagcgccccc ttttggagta attgccgaaa gccgttagat
     7201 tctggcaatt aagacaacgt gaacatagac gatggctaac ggtaacgcta gcattaaggg
     7261 ttataactgc aacgtatctc aaggacttgt cattattatg accctgcccg attttcgctt
     7321 tatccgcctg ctaccgctag ctgccctcgt gctcactgcc tgttctgtta ccacacccaa
     7381 aggcccaggc aaaagcccgg attcgccaca gtggcgtcag caccagcaag acgtgcgtaa
     7441 tcttaatcag tatcagactc gcggtgcatt cgcttatatt tccgaccaac aaaaagtgta
     7501 cgctcgcttc ttctggcaac aaaccggcca ggatcgctat cgtctgctgt taactaaccc
     7561 attgggcagc acggaactgg agctgaatgc tcagccgggt aacgtacaat tagtcgacaa
     7621 taaaggtcag cgttacaccg ccgatgacgc cgaagagatg attggtaaac taaccggaat
     7681 gccgattccg ctcaacagct tgcgccagtg gattttaggt ttaccgggtg atgcgaccga
     7741 ttacaaactg gacgaccagt accgcctgag cgaaatcacc tacagccaga atggcaaaaa
     7801 ctggaaagtt gtttacggtg gttatgacac aaaaactcaa cctgcgatgc cagccaatat
     7861 ggaactcacc gacggtggtc aacgcatcaa gttaaaaatg gataactgga tagtgaaata
     7921 atgcggacac agtggccctc tccggcaaaa cttaatctgt ttttatacat taccggtcag
     7981 cgtgcggatg gttaccacac gctgcaaacg ctgtttcggt ttcttgatta cggcgacatc
     8041 atcagcattg agcttcgtga cgatggggat attcgtctgt taacgcccgt tgaaggcgtg
     8101 gaacatgaag ataacctgat cgttcgcgca gcgcggttgt tgatgaaaac tgcggcagac
     8161 agcgggcgtc tttcgacggg aagcggtgcg aatatcagca ttgacaagcg tttgccgatg
     8221 ggcggcggtc tcggcggtgg ttcatccaat gccgcgacgg ttctggtggc gttaaatcat
     8281 ctctggcaat gcggactaag catggatgag ctggcggaaa tggggctgac gctgggcgca
     8341 gatgttcctg tctttgttcg tggtcatgcc gcgtttgccg aaggcgttgg tgaaatacta
     8401 acgccggtgg atccgccaga gaagtggtat ctggtggcgc accctggtgt aagtattccg
     8461 acaccggtga tttttaaaga tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa
     8521 acgttgctaa aatgtgaatt cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc
     8581 gaggttgatg cggtgctttc ctggctgtta gaatacgccc cgtcgcgcct gactgggaca
     8641 ggggcctgtg tctttgctga atttgataca gagtctgaag cccgccaggt gctagagcaa
     8701 gccccggaat ggctcaatgg ctttgtggcg aaaggcgtta atctttcccc attgcacaga
     8761 gccatgcttt aagccgggca agctgagttt cggtgacaac gtcaccttgt tccagacgtt
     8821 gcatcgcgct ctttaataca ccgcctggaa agaatcatgc ctggcccgca cagttttcgg
     8881 cagattcttt ccaccaatgg acgcatgcct gaggttcttc tcgtgcctga tatgaagctt
     8941 tttgctggta acgccacccc ggaactagca caacgtattg ccaaccgcct gtacacttca
     9001 ctcggcgacg ccgctgtagg tcgctttagc gacggcgaag tcagtgtaca aattaatgaa
     9061 aatgtacgcg gtggtgatat tttcatcatc cagtccactt gtgcccctac taacgacaac
     9121 ctgatggaat tagtcgttat ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc
     9181 gctgttatcc cctactttgg ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta
     9241 ccaatcactg cgaaagtggt tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg
     9301 acagtggatc tgcacgctga acagattcag ggtttcttcg acgttccggt tgataacgta
     9361 tttggtagcc cgatcctgct ggaagacatg ctgcagctga atctggataa cccaattgtg
     9421 gtttctccgg acatcggcgg cgttgtgcgt gcccgcgcta tcgctaagct gctgaacgat
     9481 accgatatgg caatcatcga caaacgtcgt ccgcgtgcga acgtttccca ggtgatgcat
     9541 atcatcggtg acgttgcagg tcgtgactgc gtactggtcg atgatatgat cgacactggc
     9601 ggtacgctgt gtaaagctgc tgaagctctg aaagaacgtg gtgctaaacg tgtatttgcg
     9661 tacgcgactc acccgatctt ctctggcaac gcggcgaaca acctgcgtaa ctctgtaatt
     9721 gatgaagtcg ttgtctgcga taccattccg ctgagcgatg aaatcaaatc actgccgaac
     9781 gtgcgtactc tgaccctgtc aggtatgctg gccgaagcga ttcgtcgtat cagcaacgaa
     9841 gaatcgatat ctgccatgtt cgaacactaa tcgaacccgg ctcaaagacc cgctgcggcg
     9901 ggtttttttg tctgtaatat ccatttgtat gacctatgcc tccttcacct gccatttagt
     9961 tgacagatga tgcgctcacg gatgaaacat tattgtgaac aaaatatttt cctcacatgt
    10021 gatgcctttc cgcgctctga tcgacgcttg ctggaaagaa aaatatactg ccgcacggtt
    10081 tacccgtgac ctgattgccg ggataaccgt cgggattatt gctatcccgc tggcgatggc
    10141 gttggctatt ggtagtggtg tggcacccca gtacggttta tataccgcag ctgttgcggg
    10201 gattgtcatt gctctgacgg gagggtcacg ctttagcgtt tccggtccga ctgcggcatt
    10261 tgtggtaatt ctctatcccg tttcgcaaca gtttggactg gcaggactgc tggttgcgac
    10321 cttgctgtcg gggatctttt tgattctgat ggggctggca cgctttggtc gcctgattga
    10381 gtatattccg gtttccgtca ccttaggttt cacctcgggt atcgggatca ccatcggtac
    10441 catgcagatt aaagattttc tcggtctgca aatggcccat gtcccggaac attatctaca
    10501 aaaagtcggc gcattattta tggcgctgcc gaccattaat gtgggtgatg cagccattgg
    10561 cattgtgacg cttggcattc tggttttctg gccgcgtctg ggcatacgtt tacccggcca
    10621 ccttccggcc ttgctggctg gttgcgcggt gatggggatt gttaacctgc tcggcggaca
    10681 tgttgctacc atcggttcgc aattccacta cgtcctggcc gatggttctc agggtaacgg
    10741 tattccgcaa ctgctgccgc aactggtgct gccgtgggat ctgcctaatt cagaattcac
    10801 gctaacctgg gattctattc gcacactgct gcctgcggca ttctcaatgg caatgctcgg
    10861 cgcaatcgaa tctctgctct gcgccgtggt gctggatggt atgaccggga cgaaacacaa
    10921 ggcgaacagc gaactggttg gacagggact ggggaatatt atcgctccgt tctttggtgg
    10981 cattaccgct acagctgcca tcgcgcgttc tgccgctaac gtccgtgccg gggcaacttc
    11041 ccctatctcg gcggtgatcc actctattct ggttattctt gccctgctgg tactggcacc
    11101 gctgctctcc tggctgccac tttccgccat ggcagccctg ctgttgatgg tggcgtggaa
    11161 catgagtgaa gcgcacaaag tggtcgactt gctgcgtcat gcaccgaaag atgacatcat
    11221 cgtcatgctg ctgtgcatgt cgctgaccgt gctgttcgat atggttattg ccatcagcgt
    11281 ggggatcgtg ctggcatcgc tgctgtttat gcgtcgtatc gcacgtatga ctcgcctggc
    11341 accggtagtc gtagacgttc cagacgatgt cctggttctg cgcgttattg gcccgctgtt
    11401 ttttgctgcc gctgaaggct tattcacgga cctggagtca cgtcttgaag gcaaacggat
    11461 tgtgattctg aagtgggatg ccgttccggt acttgatgct ggtggtcttg atgcgttcca
    11521 gcgttttgtg aagcgtctgc ccgaaggatg tgaactgcgc gtgtgcaacc tggaattcca
    11581 gccactgcgc actatggctc gcgcaggcat tcaaccgatc ccgggacgcc tcgcattctt
    11641 cccgaatcgt cgcgcggcga tggcggattt ataagtacga gattgaccag tcagcgcaaa
    11701 ctgactggtc agcaaactgc attatttgtt agctatgacg gcgattatcg ctacggcggc
    11761 aacgtttgtc atagtggcga acccaccagt aacggtcgca cacctgttca tgcccgccaa
    11821 cacgggcacc cgccagccag agaatcgcac cgacgaaaat acttagcaca gcaccatgtg
    11881 cgaaatactg gggcatatta aactgtggta actggttgag gattgaatac cccacgccga
    11941 ccaccattac caccagaccc aaccccatga gcacgttacc gagtaacgaa gcgtttttgc
    12001 gtttcatatg tcacctccgg aactttctgg gttgtaacag ggaatacccc tcttccttat
    12061 gtgtaaagta tagacaacac cctgtgggtt atgtgcgggt gtgatcacaa ttacaaccct
    12121 tatttcaaca aaactttaca aataaacgcc tgaacacctt gactttctaa tagtccactt
    12181 aggcaataat tgcattatct gcatttgata cgcagttttt tgtcaagcgg ggccgcaacc
    12241 agtaaactac gcgccagtta tgtacacact caggacaaaa aaacgtgacg attaaattga
    12301 ttgtcggcct ggcgaacccc ggtgctgaat acgccgcaac gcgacataat gctggtgcct
    12361 ggttcgttga cttactggca gagcgattgc gcgctccgct gcgcgaagag gctaaattct
    12421 ttggttatac ttcgcgagtc acccttggag gcgaagatgt ccgcctgtta gtcccgacta
    12481 catttatgaa tctcagcggc aaagccgttg cggcgatggc cagttttttc cgcattaatc
    12541 cggacgaaat tctggtggcc cacgacgaac tggatctgcc tcctggcgtc gccaaattta
    12601 agttgggtag cggtcatggt ggtcacaatg gactgaaaga catcatcagt aaattgggta
    12661 ataaccctaa ctttcaccgt ttacgcatcg gaatcggtca tccgggcgat aaaaataaag
    12721 ttgtcggttt tgtgttaggc aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca
    12781 tcgacgaagc ggcgcgttgt actgaaatgt ggtttacaga tggcttgacc aaagcaacga
    12841 accgattgca cgcctttaaa gcgcaataag tcgttgtctg cggcattttt gccgagtgcc
    12901 gtgtataata ggcaaagtta tttccatttc tgcaatctgt tagcaataac aggttgatta
    12961 ttaagatatt aaggtgattt aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa
    13021 cgtcgggaaa tctaccctgt tcaacgcgct gaccaaagcc ggtattgaag cgaccaactt
    13081 tccattctgc accattgagc cgaacactgg cgtcgtacca atgcctgacc ctcgcctgga
    13141 tcaactggct gaaatcgtaa aaccgcagcg tacacttccc acgaccatgg aatttgtcga
    13201 tatcgccggt ctggtaaaag gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac
    13261 caacatccgt gaaaccgaag cgatcggtca cgttgttcgc tgctttgaaa atgacaacat
    13321 cattcacgtt tccggcaaag ttaacccggc ggatgatatt gaagttatca acaccgaact
    13381 ggcgctggcg gatctcgaca cctgtgaacg tgcgattcat cgcgtacaga agaaagccaa
    13441 aggtggcgat aaagacgcga aagccgagct ggcggtactg gaaaaatgcc tgccccagct
    13501 ggaaaacgca ggtatgctgc gcgcgctgga tttaagcgct gaagagaaag cggctgttcg
    13561 ttacctgagc ttcctgacgc taaaaccaac aatgtacatc gccaacgtca acgaagacgg
    13621 ttttgaaaac aacccgtatc ttgaccaggt gcgtgaaatc gcagcgaaag aaggttctgt
    13681 tgtagttccg gtttgtgctg ctgttgaagc agacattgcc gaactggacg acgaagagcg
    13741 tgacgagttt atgcaggagc ttgggctgga agagccgggc ctgaaccgtg tgatccgtgc
    13801 cggttataag ctgctgaacc tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc
    13861 atggaccatt ccggttggtg caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt
    13921 tgaaaaaggc tttatccgtg cacaaaccat ctcgtttgaa gatttcatca cttacaaagg
    13981 tgaacaaggc gcgaaagaag caggcaaaat gcgtgcagaa ggcaaagatt acatcgttaa
    14041 agatggcgat gtgatgaact tcctgttcaa cgtctaaatt aaatctgctg tctcatgagg
    14101 tttcactgaa tctcataaga accgtaaaac tctataaaat ccacgcaatt gcgtggattt
    14161 ttcatttcgc aatgtctcaa ttcatctcat aacaacgctg tcaaaaatga gtacatggat
    14221 gagtacaaca ttcttccatc ttcattttaa gtgagtacaa taacggtata ataaataaac
    14281 aaggccccgt tatgctcact gatacgcaat gccgcactgc taagccgaaa gaaaaactct
    14341 atcgacttaa tgatttcaat ggtctctacc tcgaagtgaa acccaatggt aaaaaggcat
    14401 ggcgctatcg gtttaaactc aacggtaaat ccagcatgtt tgcgctgggt gagtacccga
    14461 cggtaaaact tgccgaagct cgcgagaaat gcgagcaagc gcgtaagcaa gtcgcagatg
    14521 gagttagccc aacacaggct cgtcagttag ataagatccg caaggtcaac gacgcatcca
    14581 acacctttga gctaatcgct aaagaatggt tgcagatgaa agactgggcc ggaattacca
    14641 aaacgcggcg actggatatg cttgaacgtg tggtttttcc cgccatcggt aagctccctg
    14701 tcagagagat cactccacac catatactta aaattcttca ggaaaccgct aaacgaggag
    14761 caccaacagt tgccgctgaa gcccgtcgca ccatttcatc agtttttgag ttggcagtcg
    14821 cgacgcttag agctgatagc gatcccgtat ggcctgtgcg caaggccctt ccggctaata
    14881 agacacagca caaacaggca ctaaatcccc aacaaatcgg aaagttacta agctgttttg
    14941 acaatagtcg tggctcgtat caggttaact attgcatgtg gcttatgtgg tggacattgg
    15001 cgcgtcctgc agaagccacc gaagcagaat ggacagaatt caatcttgat aacgccctat
    15061 ggactattcc ggcagcgcga atgaaagccc gcagagaaca cgttattccc ctacccttcc
    15121 aagctgtcaa aatgctcaga acactacagg gattaaccgg gcatcggcag caccttttcc
    15181 cgggcagaga caatccgcta ggtccaatga catcgcactc actgcgccag ctacttaaaa
    15241 gcctcggctg gagtggtaca tatagcccac atgcaacccg gaccacagga agtacccgtt
    15301 taaacgagtt gggctatcga cctgatgcta ttgaggccca acttgcgcac gctgatacca
    15361 acaatgttcg tcgcacatac aaccatgcaa cttatcttga tgagcgtaaa gtgatgatgc
    15421 aggaatgggc tgatatgttg gacaaatggg tagcaggatt caagtagaac ttgctgcaca
    15481 aagtgaacca cgaggataga acttattcct caaggttctg gagtgccccc aagcccgtag
    15541 acaaatctgc cctatagttt gagcatagga ggagtctatg ggcacaccac gatttacccc
    15601 agagtttaag gaagaagctg tccgtcaggt cactgaacgg ggctattccg ttgccgatgt
    15661 ttctgaacgc ctcggcgtat cggctcacag cctttataag tgggtccggt cagtcagacc
    15721 ggataacagc gggcatcagg cgcaggattt actggatgcg cggacggaga ttctcaggct
    15781 taaagcgcag cttaaacgta ctgaggaaga acgggatatt ctgaaaaagg cagcgcggta
    15841 ctttgcaagg gagcccgact gaagtaccgc ttcatcaacg atcaccgtga aatctggtcg
    15901 atcgtaacaa tgtgtcgggt tctgaaggtc gcccgtgcgg gattttacgt ctggcttcat
    15961 cgccctgttt ctgccggaga aaaggataat cagcggttgc tggagctcat ccgcgatgct
    16021 tatgcactga gcggcggagt ctacggttat cgcagggttc acggcgatct tcgtgaaatc
    16081 ggtgaagtat gcagcagaaa ccgtgttgcc agaatcatga agcggaacag gatacaggcc
    16141 gtacatggct ataaggtccc gcgtggcgtc aggggaaggc cgtcactgat agctcctaat
    16201 cgcgtacaga gggaatttac gatggtaaaa cccaaccagg tgtgggtgac tgatattacc
    16261 tacatccgca cctggcaggg ttggctgtat ctggcggtgg ttatcgatct ttttgcccgt
    16321 aatgtggtgg gctggtcgat gaaaccaacg ctgtcgcgcg agctggcact ggatgcgctg
    16381 ctgatggcag tctggcggcg taagcctgca gaaaacgtca tagtgcacag cgatcagggc
    16441 agtcagtatg gcagtgatga ctggcagcgg ttctgcaggg ccaacaatct ggcgccgagt
    16501 atgagccgtc gtggcaactg ctgggataat gcggtggcag aatcgttctt cagttcactg
    16561 aaaaaagagc gcatcagaaa gcggatatat aaaacccggg acatggcccg ggcggatatc
    16621 ttcgattaca ttgaagtgtt ctacaaccgg acccggcgcc acagtcatct gggtggcgtc
    16681 agcccggagg ccttcgaaaa ggcctcaccg tgaggacaga aactgtctac cggaatgtgg
    16741 tcactccact tgagtcgtct tgctgtactc ctttggccaa tccatttagc tctccaacgt
    16801 gatgttttca acggtaacaa aagtaacaat ataacatgca gtcatttcaa cgatctcagt
    16861 gttaccaccc attttgagca atcatatcaa agtaacaaaa ggggaatatt gtgtggttca
    16921 tcgctgaaag caggctaaga tcacctgccg gctatgcata tcatgaaagt gctaaatgtc
    16981 ccgtcttgtt tatcaaccga gataagctgt aattgactga gtggctcccc ttaatgcttt
    17041 ttctcaatca acagatatgg atgggaccag tcagtgacct ctttatatga aaaaaaccca
    17101 tcgtgctcca gtgcgcaaac gcccttatga ctggcaacgt aatcgtaaac agcccggctt
    17161 tccagtccct gtaacatgtg cgaaagcagc tttcggcgca tcacatctga cttcctgcgt
    17221 ttactgtcaa caacgggtaa taaagtgatc cactaaccgt caccaccaac ggtttaatat
    17281 tgatccacct tgtttactca ggattagctt caggtataac cccggccttt cgtttctact
    17341 tcagtcgata gctctctcct tttatctgga ctacgtgtga gtgatgtaag atacggtcca
    17401 gcatcgccga tgtcagggct gcatccctgg cgaacgtctg atcccactgc ccgaacggca
    17461 ggttggagtc caggatcatc gcgctcctct cgtaacgttt agcgatgacc tggaagaaca
    17521 gcttggcttc ttccagactg aacggcagat aacctatttc atcgatgata agtagcttcg
    17581 gggccatgac accgcgattg agtgtcgttt tgtaacggcc ctggcgctat gaagtggata
    17641 gctgtagcag caggtccgct gttgtgaagc gaaccttgat gcctgcccgt agcggcggac
    17701 gcccatggca caggtgccgc aggcgagcat gcgttcaacg gagagtcggg tccattagct
    17761 gatgctgtca gcgtattttt cgaggaattt atgtaagaag cgtcaaaatg aaagcgccaa
    17821 ctaataatga cagaattaat aaaataccag taagcgcgta tgctatacct tttccaatct
    17881 cgttttttat ggtacgtcca attgaataaa ggattaaaga taccgatagc aaa
//
LOCUS       DACADA010000089        17757 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.091, whole genome shotgun sequence.
ACCESSION   DACADA010000089 DACADA010000000
VERSION     DACADA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.091"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(201..1229)
                     /gene="epmB"
                     /locus_tag="ISB17_002975"
     CDS             complement(201..1229)
                     /gene="epmB"
                     /locus_tag="ISB17_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP1980877.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1271..1837
                     /gene="efp"
                     /locus_tag="ISB17_002976"
     CDS             1271..1837
                     /gene="efp"
                     /locus_tag="ISB17_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP1980878.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1889..2014
                     /gene="ecnA"
                     /locus_tag="ISB17_002977"
     CDS             1889..2014
                     /gene="ecnA"
                     /locus_tag="ISB17_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP1980879.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2125..2271
                     /gene="ecnB"
                     /locus_tag="ISB17_002978"
     CDS             2125..2271
                     /gene="ecnB"
                     /locus_tag="ISB17_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP1980880.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2447..2764
                     /locus_tag="ISB17_002979"
     CDS             2447..2764
                     /locus_tag="ISB17_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP1980881.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2761..3294)
                     /locus_tag="ISB17_002980"
     CDS             complement(2761..3294)
                     /locus_tag="ISB17_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP1980882.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3383..4516)
                     /gene="blaEC"
                     /locus_tag="ISB17_002981"
     CDS             complement(3383..4516)
                     /gene="blaEC"
                     /locus_tag="ISB17_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP1980883.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            complement(4579..4938)
                     /gene="frdD"
                     /locus_tag="ISB17_002982"
     CDS             complement(4579..4938)
                     /gene="frdD"
                     /locus_tag="ISB17_002982"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP1980884.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4949..5344)
                     /gene="frdC"
                     /locus_tag="ISB17_002983"
     CDS             complement(4949..5344)
                     /gene="frdC"
                     /locus_tag="ISB17_002983"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP1980885.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5355..6089)
                     /locus_tag="ISB17_002984"
     CDS             complement(5355..6089)
                     /locus_tag="ISB17_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP1980886.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6082..7890)
                     /gene="frdA"
                     /locus_tag="ISB17_002985"
     CDS             complement(6082..7890)
                     /gene="frdA"
                     /locus_tag="ISB17_002985"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP1980887.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8215..9192
                     /gene="epmA"
                     /locus_tag="ISB17_002986"
     CDS             8215..9192
                     /gene="epmA"
                     /locus_tag="ISB17_002986"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP1980888.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9411..10913
                     /gene="yjeM"
                     /locus_tag="ISB17_002987"
     CDS             9411..10913
                     /gene="yjeM"
                     /locus_tag="ISB17_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP1980889.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            11011..11325
                     /locus_tag="ISB17_002988"
     CDS             11011..11325
                     /locus_tag="ISB17_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HAP1980890.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11356..14679)
                     /gene="mscM"
                     /locus_tag="ISB17_002989"
     CDS             complement(11356..14679)
                     /gene="mscM"
                     /locus_tag="ISB17_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP1980891.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14701..15669)
                     /gene="psd"
                     /locus_tag="ISB17_002990"
     CDS             complement(14701..15669)
                     /gene="psd"
                     /locus_tag="ISB17_002990"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP1980892.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15766..16818)
                     /gene="rsgA"
                     /locus_tag="ISB17_002991"
     CDS             complement(15766..16818)
                     /gene="rsgA"
                     /locus_tag="ISB17_002991"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAP1980893.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16913..17458
                     /gene="orn"
                     /locus_tag="ISB17_002992"
     CDS             16913..17458
                     /gene="orn"
                     /locus_tag="ISB17_002992"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP1980894.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17669..17744
                     /locus_tag="ISB17_002993"
     tRNA            17669..17744
                     /locus_tag="ISB17_002993"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17702..17704,aa:Gly,seq:gcc)
BASE COUNT         4091 a         4539 c         4669 g         4458 t
ORIGIN      
        1 ttaattttag tttataagcc agataaatgg gcttggtagt aatagttgtt aaaataacat
       61 aaatagccgt gctcactcta tataaacaga aaagaaaggt taattattgg tgtcggttat
      121 ataaatagta ataacagcaa tgtatatgaa taattaaagg ttatgcaacg ggcaaagatt
      181 aaacttccct gtttaatcag ttactgctgg cgtagctgga gatccagcgg tgttttgctg
      241 ggttcgccgc caatttctcg cgccagttta ggcaccagat atcccgacac cagtgtcagc
      301 aactcacgca taatctgccg tgcttcgtca tcactcacca taaaatgcgc cgcgccctgt
      361 actttatcga gcacatgcag gtaatacggc atcacacccg catcgaacaa cgcattactc
      421 aggtttgcca gcgtttgcgc gttatcgttc acaccacgta acagaacgct ctggttcagc
      481 aaagtaacgc ctacccggcg caacttagcc atcgcctgac ggaatgtttc atctacctca
      541 ttggcatggt taatgtgatt caccagcaag atttgcagcg tagaatgaga aaagcgttca
      601 accagcgcat ctgtgatgcg tgccgggatc acaatcggca gacggctgtg aatccgcaga
      661 cgtttgatat gcgggatggc ttccagttgt gtgagcaacc agtccagctc gtgatctttc
      721 gccatcagcg gatcgccgcc ggagaaaatc atctcgtcca gttccggatg cgcagcaaca
      781 tactcaagtg cagtctgcca gttacgcttg ttgccctgat tttcggcata ggggaagtgg
      841 cgacggaagc aatagcggca atttaccgcg cagccgcctt tgaccagcaa aagcgcccgg
      901 ttgtggtatt tatgcaacaa acctggcact acgctgtgct gttcttccag cgggtcggtg
      961 gagaatccgg gcgcgacaac aaactcatct tgcgaggtaa ttacctgacg caaaagcgga
     1021 tcgttcggat tgcctttctc catgcgatcg ataaatgagc ggggcacacg cagggcaaac
     1081 agctttttgg cgctgcgtcc ggccaacagt ttttcgtccg cgtctatatt caaaagacgc
     1141 agaagttcat caggatcggt cacaacatcg gcaagttgcg ttaaccaatc ttctctggat
     1201 ggggtattta gggttacaat atgcgccatt ttgtggctta gctaccaatt aacaaatttc
     1261 agagggcctt atggcaacgt actatagcaa cgattttcgt gctggtctta aaatcatgtt
     1321 agacggcgaa ccttacgcgg ttgaagcgag tgaattcgta aaaccgggta aaggccaggc
     1381 atttgctcgc gttaaactgc gtcgtctgct gaccggtact cgcgtagaaa aaaccttcaa
     1441 atctactgat tccgctgaag gtgctgatgt tgtcgatatg aacctgactt acctgtacaa
     1501 cgacggtgag ttctggcact tcatgaacaa cgaaactttc gagcagctgt ctgctgatgc
     1561 aaaagcaatt ggcgacaacg ccaaatggtt gctggatcag gcagagtgta tcgtaactct
     1621 gtggaatggt cagccgatct ccgttactcc gccgaacttc gttgaactgg aaatcgtgga
     1681 taccgatccg ggcctgaaag gtgataccgc aggtactggc ggcaaaccgg ctaccctgtc
     1741 tactggcgct gtggttaaag ttccgctgtt tgtacaaatc ggcgaagtca tcaaagtgga
     1801 tacccgctct ggtgaatacg tctctcgcgt gaagtaatgc ggttgtggtg cggcctgcag
     1861 gctgcaccat cacttattca ggttagagat gatgaaacgc cttatcgttc ttgttttgct
     1921 tgccagcacg ctgcttacgg gctgtaacac cgctcgaggt ttcggcgaag atatcaaaca
     1981 cctcggcaac tccatctccc gcgctgccag ctaatttttc ttctcttccg aaaaatcatc
     2041 agatttccgt catttttggc gatgttgtct attattaatt tgctataggc aaacataaat
     2101 aacattacct aaaaggaaga cgttatggtg aagaagacaa ttgcagcgat cttttctgtt
     2161 ctggtgcttt caacagtatt aactgcctgc aacaccacgc gtggcgttgg tgaagacatt
     2221 tctgatggcg gtaacgcgat ttctggcgca gcaacgaaag cgcagcaata agcaataacg
     2281 gtacgacagc tgtgtcgtgc cgtttgtttt ttctgcgata gttacatagg taatagttga
     2341 aattcccctg ccacctggca aaatatccgt tcaaccatca gctttgcagg acgacctgca
     2401 aacgcgtctt ttcaccgggg acggccccaa ttttccggag cctgatatgt cctggattat
     2461 cttagttatt gctggtctgc tggaagtggt atgggccgtt ggcctgaaat atacccacgg
     2521 ctttagtcgt ttgacgccaa gtgttattac cgtgacggcg atgattgtca gtatggcgct
     2581 acttgcctgg gcgatgaaat cgttaccagt agggacggct tatgccgtgt ggacgggtat
     2641 tggcgcagtc ggcgcggcca tcaccggcat tgtgttgctc ggtgagtccg ctaacccgat
     2701 gcgtctggcg agtctggcgt taatcgtatt ggggattatt ggtctgaaac tcagcactca
     2761 ttaactaccg ggctgctgta cccaaataaa cttactgaca tcaaaccctt cccgggtcgc
     2821 gactgccagc atctcctgtt tcacttcgtc agaaatggtt ggcgtgcggg agagtatcca
     2881 caggtagtcg cggtccgggc cgcaaaccag cgcatggcgg tattcccgat cgagtgcaat
     2941 aacgttataa ccgccataga aaggaccaaa gaatgacact ttcagcgcag cgcgagttgg
     3001 tgcgccggta aagtacgctt tcccttcact ctgctgccac atttctctgt cagggttata
     3061 gcctttatta atgacattca ggccgccgtc atcacgcagg ctgtatgttg cggtgacttt
     3121 ttccagtcca cgttcaaagc ggtgatcaaa acgggcaatc tcataccagg tgccgagata
     3181 gcgtttagcg tcgaaattat tcactacggt cacgccacgc ggcggtgtag gagaactgca
     3241 ggcaacgacc agaaatgcag ctgtcgctgc ggcaacgaga gggagcaggc gcataaatgt
     3301 ttccttactg gtttttttct aagtgtagat gacagcaagg aaaagcggag aaaaggtccg
     3361 aaaattcgga cccgatgaaa ttttactgta gagtgttgag aatctgccag gcggcggtaa
     3421 ctctcgctgg attgggatag tttttgttag ccagcatcac gatacccagc tctttttctg
     3481 gaataaacgc gacatagcta ccaaatccgc cggtcgcccc cgttttatgt acccatgatg
     3541 cgcgtactgc aggagttggg ggcgtaatcg gttttacggg gcgtgctgcc agtgcaattt
     3601 tattgtcact gccgttaatg atgatgtcag gatttaccgg ccagtccagc atttcccagc
     3661 ccagaccctg atacatatcg ccggtttgcc agtagcgaga ttgtgccagt tgtatccctt
     3721 gttgaagtat tttctcattg atatcaaggg gttttaaatt gctttgcacc cagcgggcca
     3781 tatcttcaat ggtcgacttc acaccataag cttcagcatc taacgcccct ggcgaaacat
     3841 gtactgcctt accttcgcga tatccccagg cgtagttctt ttcttctgcg gacggtacat
     3901 taatccacgt atgattgagt ttgagtggct ggaagacacg agttttcatc gcctgctcaa
     3961 aactcaaacc agacggcttc acagccagtg cgccgaacaa accgatactg gagttggcat
     4021 acagacgttg tgttcctgga gcccatgcag gctgccagtt ttgatagaag cgcagcaagt
     4081 cgcttgagga tttcacctca tccggcacct gcaatggcag gccgccagcg gtgtaggttg
     4141 cgagatgtaa tagtgtgatc ccattccact gtttagcggt aagttcaggc cagtattttg
     4201 ttgtgggatc gcttaacttg atttcccctc gagcaatagc gtcgccacca agcacgcccg
     4261 taaatgtttt gctgaccgaa cctaactcaa acaacgtttg ctgtgtgacg ggctgctttt
     4321 tggcgatgtc cgcatagccc caggtaaagt aataaggttt accctgataa attaccgcta
     4381 ccgccatacc ggggatcctt tgttgctcta taagcggggt aattgtgcga tgcacaatat
     4441 cgttgatttg ttgaggggcg gcaaatgtgg agcaagaggc ggtaattaat aaggtgcaga
     4501 gcgtcgtttt gaacataggg tctggtttcc atacaaaacg gcccgccata ggcgggccgg
     4561 atttacattg gcgatgcgtt agattgtaac gacaccaatc agtgtgacaa ctgtcaggat
     4621 agcagccaga ccgtagaaaa cccatttacc cgcaggtacg tggattttca gatcgtgcat
     4681 cgcgtggtgc attcggtgta aaccacacca cagcggcaga acgatcatca ggaacaggaa
     4741 tacgcgacca atgaagctct gcgcgaacgc cagaacgcgc tcgtagctca gcgcatcacc
     4801 tggaaacagc cctagcggta gcagaatacc aaccagtagg atcatcaccg gcgcaatgat
     4861 ggcgctccac ataccaccgg ccccgaagag gccccagaat accggttcat cagaacgctt
     4921 tggatttgga ttaatcatct caggctcctt accagtacag ggcaacaaac aggattacga
     4981 tggtggcaac cacagttacc gcccagagac ttttgataat tggctctggt cccatttttt
     5041 cgtcttttac aatgatattg gccgcttttg gtgccagctc aaaccaggtt ttggtgtgca
     5101 gtagggctgc cgccagagtg atcaggttaa tgatcacgat aaccgggttt tgtaaaaagt
     5161 cgacgaatcc cgcccaggct tccgggccat ttttcagggc aaacagcccg aaaatcagtt
     5221 caatgctgaa ccacacagcc ggaactgccg tgccttcgcg cagcatgtaa aagcgataaa
     5281 acggcaattt tttccaccag gtggacgtca ttggccgtac atacggttta cgtttagtcg
     5341 tcatgttgca ctccttagcg tggtttcagg gtcgcgataa gaaagtcttt cgaactttct
     5401 actttgccct gctgaatggc cgcagccgga tcgacgtgtt tcgggcagac ttcggagcag
     5461 tagcccacga aagtacagct ccatacgccg ttctggctgt tcaactgcgc catacgctcc
     5521 ttcttaccgt ggtcgcggct atcttcgtta taacgatgcg ccagcgtaat ggcagccgga
     5581 ccgatgaact ctgggttcag gccaaactgc gggcacgcgg cgtagcacag accacagttg
     5641 atgcaaccgg agaactggtg atacttcgcc atctgcgccg gggtctggat gttagtaccc
     5701 tgatccgcgg tgcgggagtt gccgatgatg tacggtttga tcgcttccag actttcgatg
     5761 aagtgggtca tatcgaccac cagatcgcgt tcaatcggga agttagctaa cgcttcaacc
     5821 ttcataccgt cggtgtaatc acgcaggaag gttttacatg ccagttttgg cacgttgtta
     5881 accatcatgc cgcaggagcc acaaatcgcc atacggcagg accagcggta gctcaggtcc
     5941 ggtgccaggt tgtctttgat gtagcccagc gcatccagta atgaggtagt tgcgtcataa
     6001 ggcacttcat agaatgcgct atgcggtgcg gtatcgactt ccgggttata gcgcaccacc
     6061 tcaattttca ggtttttcat ctcagccatt cgccttctcc ttcttattgg ctgcttccgc
     6121 cttatcggct gcatccgctt cgccaccgta aacacgttta gctggcggca gcgtagtaat
     6181 cttcacgtcg ctgtactcca ggcgagtcgt gccatcagca tcgcggaagg cgagggtgtg
     6241 tttgaggaag ttgacgtcgt cacgctcggt gcaaccttcg tccagacgct ggtgcgcgcc
     6301 gcgggactct ttacgtgcca ttgcggagtg cgccatacat tcagcaacgt tcagaccgtg
     6361 gcccagctca atggtgtaga gcaggtcggt gttgaacacg ctggaagtgt cggtgatgcg
     6421 cacgcgcttg aagcgttcct gcagctctgc cagcttgtcg atggttttct gcatcagttc
     6481 cggcgtacgg tagataccgc agccttcttc catggccatg cccatttcgt cgcggatctt
     6541 agcccagttt tcgccgccat cctggttaac cagatctttc agacgttgtt caacgccagc
     6601 tgcctgcgct tcaattgccg cttcgttgcc attaccggca gttgctgcac gctctgtcgc
     6661 ttgttcaccg gccagacgac cgaagaccac cagttccgcc agcgagttgg agcccagacg
     6721 gttcgcaccg tgcagaccaa cagaggaaca ttcacccacg gcgaacagac ctttaatgcg
     6781 ggtttcacag ttctgatcgg tttcgatacc gcccatggtg tagtgtgcgg tcggacgtac
     6841 cggaatcggt tctttaaccg gatcgacgcc aacgtacgct ttcgccagtt cgcagatgaa
     6901 cggcaaacgt tcatgcagtt ttttctcgcc aaggtgacgc aggtcgagat aaaccacatc
     6961 gccacgcggc gtggagatgg tgttgccttt acgccattcg tgccagaagg cctgagaaac
     7021 tttgtcgcgt ggacccagtt ccatatattt gtttttcggc tcgcccagcg gagtttccgg
     7081 gcccatgccg taatcttgca gataacggta gccatttttg ttgaccagaa taccgccttc
     7141 accgcggcag ccttcggtca tcaggatacc ggaacctggt agaccggttg ggtgatactg
     7201 aacgaattcc atgtcacgca gcggaacgcc gtggcttagc gccataccca taccgtcacc
     7261 ggtaacgatg ccgccgttgg tgttgtaacg ataaacgcga cctgcaccgc cggtagccat
     7321 aacgaccgcg ttagcacgga tctgcaccag cgtgccttcc atcatgttca ttgctaccag
     7381 gccgcgaaca tgaccatcat caactagaat atccagcacg aaatgttcgt cgaaacgctg
     7441 gatctgcggg aattgcagag aggtctggaa cagcgtgtgc agcatatgga agccggtctt
     7501 atcggcggcg aaccaggtgc gctcgatttt catgccgccg aagcgacgta cgttgacgct
     7561 accatccggg cgacggctcc atgggcaccc ccacagttcc agttgggtca tttcggttgg
     7621 gcagtggtgg acgaaataat ccacgacatc ctgctcacac aaccagtcgc cacccgctac
     7681 tgtatcgtga aagtgatatt cgaagctgtc atgatcctgc gcgacagcgg cggagccccc
     7741 ttctgcagca acggtatggc tacgcatcgg gtatactttt gagattagtg cgatttttgc
     7801 atttggattt gcctgcgcgg cagcaattgc agcacgtaat cccgcgccac cggcgcctac
     7861 aatggcaaga tcggcttgaa aggtttgcac gacattcctc cagattgttt ttatcccaca
     7921 gccccgtact tcagggtaag tgcctgaaag ttacggtctg cgaacgctat tccactgctc
     7981 ctttataggt acaacagtat agtctgatgg ataagtctga aatttgacga gatcgatttt
     8041 tttagtgcgc aagagggcta aattatcact gaagatgatt aatttaatta ctaaaccatc
     8101 agatcgtgct ttttttagtc actgcccacc gttgtgttat tcctgcgtaa aatttgtctc
     8161 gccgtcgcgt tgcgagtaga cttcgtgccc ttgtcaaaaa ctggagattt aactatgagc
     8221 gaaacggcat cctggcagcc gagcgcatcc attcctaact tattaaaacg cgcggcgatt
     8281 atggcggaga tccgtcgttt ctttgccgat cgtggagtgc tggaggtgga gacgccttgt
     8341 atgagccagg cgacggtaac cgatattcat ttggtcccgt ttgagacacg tttcgttggc
     8401 cccgggcatt cgcaggggat gaatctctgg ttaatgacca gcccggaata ccatatgaag
     8461 cgcctgctgg ttgccggttg tgggccggta ttccagctgt gccgcagctt ccgtaatgaa
     8521 gagatggggc gttatcacaa ccctgagttc actatgcttg agtggtatcg accgcactat
     8581 gatatgtacc ggttgatgaa cgaggtggac gatctcttgc aacaggtgct ggactgtccg
     8641 gcagcagaaa gcctttctta tcaacaagct ttcttgcgtt atctggaaat tgacccactc
     8701 tctgccgaca aaacgcaact gcgggaagtg gcagcgaaac tggatttgag caacgttgcc
     8761 gatactgaag aagaccgcga cacgcttcta caattgctgt ttacctttgg cgtagagcca
     8821 aatattggca aagaaaaacc gacctttgtg taccactttc cagccagcca ggcatcactg
     8881 gcgcaaatca gtaccgaaga tcatcgggtc gctgaacgct ttgaggttta ttataaaggt
     8941 attgagctgg cgaatggttt ccatgaattg acggatgccc gtgagcagca acaacgcttt
     9001 gaacaagata accgtaagcg cgcggcgcgc ggtttgccgc agcaccccat tgaccagaat
     9061 ctgattgaag ccttgaaagt cggtatgcct gactgttccg gcgtggcatt aggtgttgat
     9121 cgtctggtga tgttggcgct gggcgcggag acactggctg aagtcatcgc ctttagcgtt
     9181 gaccgggcat aattctgaaa aattactgaa ttaacagcga agaatggcgt gataagcgcc
     9241 attctgtagc atatgttttc atttttccgc taaaggccac aatttaagac tggatatttg
     9301 atatcatcca ggtatcaatt ctgtattggt ttttacgctg cccgctcaat gttgggcagt
     9361 gcagttcagt gggcgcgttt tatacacgcg ctgaaatgaa ggatggtttc atgcctcact
     9421 cgataaaaaa gatgagtctg ataggactca tattgatgat ctttacttct gtatttggat
     9481 ttgccaatag cccatcggct tattacttaa tgggttatag tgcgatttcc ttttatatat
     9541 tttctgcatt gttattcttt attccattcg ccttaatgat ggctgaaatg ggagctgctt
     9601 atcgcaaaga agaaggcggt atctattcct ggatgaataa tagtgtcgga ccacgttttg
     9661 ccttcattgg tacgtttatg tggttttcct cttatatcat ctggatggtg agtacctccg
     9721 cgaaagtttg ggtaccgttc tcaacattcc tctacggtag cgacatgacc cagcactggc
     9781 atattgccgg actggagcct acgcaggtgg ttggtctgct ggcagtggca tggatgattc
     9841 tggtcaccgt cgttgcttca aaagggatta ataaaattgc ccgcattact gcggtgggcg
     9901 gtattgcagt aatgtgtctg aatttagtat tgctgttagt aagcattact attttgttat
     9961 taaatggtgg gcatttcgcg caggatatta atttccttgc atcaccgaac ccaggttatc
    10021 agtccggtct ggcaatgcta tcgtttgtgg tatttgccat ttttgcctat ggcggaattg
    10081 aagcggttgg tggtctggtt gataaaacgg aaaatccaga aaagaacttt gccaaaggta
    10141 ttgtttttgc cgctattgtt atttcaatcg gttattcgct ggcaatattt ttatggggcg
    10201 tcagcacaaa ctggcagcag gtattaagta atggttccgt taacctcggc aatattacct
    10261 atgtgctgat gaagagcctc ggggtgacgc tgggtaacgc actgcatttg tcacctgaag
    10321 cgtcattgtc gctgggcgta tggtttgcgc gtattaccgg actgtcgatg ttcctcgctt
    10381 ataccggtgc gttctttacg ctttgctatt caccgctgaa agccatcatc caggggacgc
    10441 cgaaagcatt gtggccggaa ccgatgacgc gcctgaatgc gatggggatg ccgtctatcg
    10501 ccatgtggat gcagtgtggg ttggttactg tcttcatcct gctggtttcg tttggtggcg
    10561 gtaccgcatc ggcgttcttt aacaagctga cgctgatggc gaacgtgtct atgacgcttc
    10621 cttacctgtt cctcgcgctg gctttcccgt tctttaaagc acgtcaggat ctcgacagac
    10681 cgtttgtgat tttcaaaacg cgtatgtcgg caatgattgc gacagtggtt gtcgtactgg
    10741 tggtgacatt tgcgaacgtc ttcaccatca ttcaacctgt ggttgaagcc ggagactggg
    10801 acagcacatt gtggatgatt ggcggccctg tcttcttctc gctgttagcg atggcgattt
    10861 accagaacta ttgcagcaga gtggtaaaaa atccgcagtg ggtggctgag taatcaaatg
    10921 ccctatcgtc ggcctgacga tagggcgtaa tgacagggaa ttagtgagat ttcgtatgcg
    10981 ctatagcgaa attaaactat gggttacctc atgagtgcgc gcatgtctgt cttgtgctgc
    11041 atctggttca ttgtggcgtt cctctggata accattacct ccgcgctgga caaagagtgg
    11101 atgattgatg gacgcgggat taacaacgtc tgtgatgttt tgatgtatct cgaagatgac
    11161 gatactcgcg atgtcggtgt gataatgacg ttaccgctct ttttcccttt tctctggttc
    11221 gctttgtggc gaaaaaaacg cggctggttt atgtacgcca ccgcgctggc tattttcggc
    11281 tactggttgt ggcagttttt tctgcgctat cagttttgct tgtgagccgg atgggttcat
    11341 ccggccacaa agaccttaca aacttcccgc ctgacgacct ttgcccgcag aagttaatgt
    11401 tctccccgtt tgtttaccat tcaggctttc cagacgcatc tggaagggcg ggaatggcat
    11461 atcgataccg tgggcgtgga agccagccag aatcagctgg tgaatctcat ggcgtagcgg
    11521 catacggtga cccatctcag cggcgtaaat acgcagctcg aaaatctgaa tcccctgctg
    11581 cagatccacc aggaagactt ccggcgccgg gttgtcgatc accagcgagc agcgacgcgc
    11641 tgcggtgagc aggatttccg tcacttcttc actgttggca ttggcagggg ccggaatcgt
    11701 caacaccacg cgcgtgaccg agtcagagag cgaccagttg ataaactgct cggtaataaa
    11761 cgccttgttc ggcacgataa tttctttgcg gtcccagtcg ctgatggtgg tggcgcgggt
    11821 gttaattttc gtcacactac cggtaagatc gcggatcgtt accgtatcgc caatgcgaat
    11881 cggtttttcg aacaggataa tcaggccaga gataaagttg gcgaaaattt cctgcaaacc
    11941 aaaaccgaga ccaacaccga gcgcggcgac cagccactgc aatttcgacc actcaatacc
    12001 aatcatcgag aagccgacca gcccgccaat tagcatcagc agatatttgg tgatggtggt
    12061 gatggcgtag cccgtacccg gcgttaaatc caggtgctgc agaatcgcca gttccagcag
    12121 cgcgggcagg ttgcgcacca gctgcgtggt gatgataaac accagaatgg caatcagcac
    12181 cgcaccgagg gtaatcggct ccaggctttc tacgccctgt accgtggaag tgacatccca
    12241 cagcgaaata ttttcgagga agccgaaagc ggaatggatt tctgaccaca gcacaatgac
    12301 cgaaagcagg gcgatcaaca tcaaaattga gcgcaccagc cgcaaggatt gcgcactgat
    12361 ggcatcgaga tcgacttcgc tttcatcgac ttcaattgct ccttccgggc tactgtgatg
    12421 atgcgcttcc tcttcgccac gcgcacgctg cgctaacatc tctgcccggc gatgctttgc
    12481 ccggtcaaac gccagcctgc ggcgctggat cagcatccag cggcggataa cgtgataaac
    12541 caccagtagc aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca gcgcctgcgc
    12601 cgttgccaga taacccaccg ccgacgccag aatggcaacc agaggtgcgc caatcatcat
    12661 gttccacagc atatggttgg taatgttgtc gccgctgcct tctttgttga gatacagcgg
    12721 gatcccggcc tttttcaggc tgagggtgac caccgccagc gcaccgcaaa tgaggataaa
    12781 gcaaagccgt cccagcgaac cggaaaattc gcggtcgtcg aggttatcga acatcatcag
    12841 cgccataatc agcggcacaa taagcccgat gctcatcagg tagtaacgca tcccacggga
    12901 aacacgttcg cgcggccagc caaaatgagc gataaacaag ccgttcgggc gagcaaaggt
    12961 ggcgcaaatc atcactaccc acagcagcgg cacggtggct gttacgccat caccaatcgc
    13021 gaccgccagc ggataaggcc acgcctcgcg caagccgtaa cccagcgtca tccacagcac
    13081 aggcagcggt gacgcgacga gaatcgacca gaaaagagtg cgtaacgtca gccagaagtg
    13141 atcctgagtc actttgccga ctttcgccgc cgaacgttca agaaaacggg tgaaatagcg
    13201 gcgcgagtaa atactgcaac cgaccagaat caacgcgcca aacagcggca aaatcgtctc
    13261 tttactggtc agcatcatca cactggcttt gcccaactga ctgaaggtgt ccagcgaaat
    13321 gagacgacgc agatcctggg cgatttccag cggccaggcg atggtcatcg ggcgcacgtc
    13381 agaggtccag aacagatagc ggtgcgtcgc ttcgttcacc tctttcagtg catcctccag
    13441 ttgcccgttg gagactttca gcttggtcag ctccagcagt agcgtgtcgc caccctgcaa
    13501 caatgagttc agcaactcac gctgagtgcg tagctgtgct tccagaatac ggttttgctc
    13561 agcggtcagc ggctgaccgt cggcctgatg gatttgccgt agcagcggct gtttattaag
    13621 cagatcctca taacgtaacc gttgcacacg taactgcgcc atttcggtat caagctgttg
    13681 tggttttggc atttccggta gccgtgccac ctgcgcccgc aacgcttcgc cgagcaggtt
    13741 agatgatccc agccattgcg actgttcacg cagcgtattc aacgcctgcc ggacctgtaa
    13801 cgtctggctg gcagcctgac gctgttgcga ggcaacgaga tccatccgct gcgcctgttg
    13861 attcagtgcc gccgatagtt cgcggttaat tttaaactgg gcgacgatat cttttggcaa
    13921 atcggcgcta ctttccgcca gttgctcggt actttccagc gcccgttccg cttccagttg
    13981 gcgttgacta ttcaactggt tgcgtagtgc ttgcaaatat gcatccagct gctggctctc
    14041 tttttcggcc agctctgagc gcaagcgcgc taattcctgg cggttattgg cagatagctg
    14101 cgccagctcc agttcatcaa cgagcgcttt cagacgtgcg gagtcagact gcaacgcgaa
    14161 attttgtgcc tgattgagcg gggtattgcc ggtaagcgtc cccaggcggc gctcgatttc
    14221 attcaactga cggcgggcat cggtttgctg ttgcggcagt tgattcaggg aatcggcaat
    14281 ctcgcgggcg cgctcctgct cttgctgggc ctgacggctt ttatccagca actgactgct
    14341 gatctggaga atttcctgat tcagcgcatc gttagacatt cccggcgaca cgctgcgtgg
    14401 ctcgtcacgc atgttgttta actgcgcgcg cagagtggcg gaaagtttcg ggtaattatc
    14461 aatgacttct tgatattgct tgatgcgctc aagggaacct tttcgttcct caagcgcatt
    14521 taaggcagac tggagcgcct ctacgacttc cggctgcgca ggtttcgccg cttttgcctg
    14581 ctccagttcc tgggtgattt gtttgctatc gggggccgtc gcggcgtacg ccccccaact
    14641 gaggcaccag gccatcagaa aagtgataat caggcgcacg tcagcgtttc ctttgatgga
    14701 ttagacctgg tcttttttat cgtcaaccaa tgggctggcg tcgtgttctg cttcgatctc
    14761 ttcagcagga agcggggctg gttcagcatc tggcgtaaca aaggtttcgg tagatactgc
    14821 cagcggctgg ccaattttcg tgacagacag gctctccagt tgctcaacca ggtccacttt
    14881 acctggtgca aacaggttga taacggtaga gcctagttta aagcgaccca tttcctggcc
    14941 tttcagcagt gccacagaac cgtcgttttc cccggcaggc caggtccagc gcttgatgat
    15001 gccttcgcgc ggcggcgtaa tggtgcccgc ccataccgtc tcaatgctgc ctacaatcgt
    15061 cgcaccgacc agaatctgcg ccatcgggcc aaattcggta tcgaacaggc agatcacgcg
    15121 ttcgttacgg gcaaacagat tcggcacgtt ctgagccgtg agatggttaa cggagaagag
    15181 atcgcccggc acgtagatca tttcacgcag aataccgttg cacggcatgt gtacgcggtg
    15241 gtagtcacgt ggggagaggt aagtggtcac aaacgtaccg ttacggaaga gatccgccat
    15301 cagatagttg cctgccagca gggcttcgag gctgtagttg tggcctttgg cttgcaggat
    15361 tttatcttct tcgattttac ccaactggct gataacgcca tcggcaggca taaccaggac
    15421 attcggatcg gtatcgattg ggcgtacttc atcacgcagc ggacggacaa agaattcgtt
    15481 aaaggtgcgg tagctggcgg tgtccggctt ttgcgcctct ttcatgtcga ccttgtagta
    15541 tttaacgaac agatcgataa ccagttttgt cagccatcct gcccgcttgc ttgcgcccca
    15601 acccgccagg cgagtaagcc atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga
    15661 atttaacaag gtagcctcca ggccattgtt ttgtcgttcc tgatccggcc tacatgccgg
    15721 atcctgaaaa aaaggggacg attctaacga cggttagctt aattgtcagt catccgtatc
    15781 agaaaagttt ttacgcgttt ttacctgcgc catgctttcc agaatacggt gatagttttc
    15841 gaaacgggtt tccgcgattt tcccttcctc aaccgcttcc cggatagcgc agcccggatc
    15901 ggtatcgtgt ttgcaatcgc gatatttaca cagacctaaa tagtcatgga attcgacaaa
    15961 gccctgagtg atttgttccg gctccaggtg ccagagacca aactcacgca cacctgggga
    16021 atcaatcaca tcaccgccgt gcgggaagtg atacagtcgt gcggcggtcg tggtgtgctg
    16081 accaagcccc gagttgtcag agacatcgtt ggtcaggatc tctttttgca gacccagcaa
    16141 cgcgttcagc aggctggatt tgccaacgcc agactgcccg gcaaaaatgc tgatgcgccc
    16201 ggtcaacgcc tcttccagcg gtttcagccc atcctgagta tgactggaaa ccatcaatac
    16261 gcgataaccg atattgcggt agatatccat ctgctcgttg acgaactcca tgccttcgtc
    16321 gtccagcagg tctatcttgt tgagcacaat aatcggctca atctgcaagg tttcgcaggc
    16381 caccaggtaa cggtcgataa tattgagcga cagctccggc aaaatggcgg agacaatgac
    16441 aatctggtcg atgttggcag caataggttt cacaccgtcg tagaaatccg ggcgcgtcaa
    16501 cactgaggtg cgctcatgca ccgcttccac gatccctttg acatttacgc cttccgccgc
    16561 cggtttaccc ggacgccaga ctacgcggtc gccggtcacc aacgaacgga tggtacggcg
    16621 aatattgcag cggtgaacgt cgccatcggc ggattccaca tcagcgtgca taccaaagcg
    16681 gctgatgacg ataccttcat caggctcgcc aaacagattg tcgtcgtagt cgggcttctc
    16741 cttagacgtt ttaagacgac gctggtgatt ggcgttcacg cggcgctgct ggcctttgga
    16801 gagtttattt ttactcaatc gtacagactc ctggtcgccc ctgatgggca aaacatctat
    16861 gatacacgca attgtggatc aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc
    16921 caatgaaaac aacctgattt ggatcgatct tgagatgacc ggtctggatc ccgagcgcga
    16981 tcgcattatt gagattgcca cgctggtgac cgatgccaac ctgaatattc tggcagaagg
    17041 gccgaccatt gcagtacatc agtccgatga acagctggcg ctgatggatg actggaacgt
    17101 gcgcacccat accgccagcg ggctggtaga gcgtgtgaaa gcgagcacga tgggcgatcg
    17161 tgaagctgaa ctggcaacgc tcgaattctt aaaacagtgg gtgcctgcgg gaaaatcgcc
    17221 gatttgtggt aacagcatcg gtcaggaccg tcgcttcctg tttaaataca tgccggagct
    17281 ggaagcctac ttccactacc gctatctcga tgttagcacc ctgaaagagc tggcgcgccg
    17341 ctggaagccg gaaattcttg acggctttac caagcagggg acgcatcagg cgatggatga
    17401 tatccgtgaa tcggtggcgg agttggctta ctaccgcgag cattttatca agctgtaaaa
    17461 tttttcctgg tcacgtaagc gccggaatcc acttgcggcg gcgtgatttt gacgctaaat
    17521 tgaacgcctt gctgataatt tcagcacttg agataaaaac tcaaaaaaaa ttttttaggg
    17581 gggttgcaga gggaaagatt tctcgtataa tgcgcctccc gtaacgacgc agaaatgcga
    17641 aaattacgaa agcaaaatta agtagtacgc gggaatagct cagttggtag agcacgacct
    17701 tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc tccaaaattt gaaaagt
//
LOCUS       DACADA010000090        17749 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.092, whole genome shotgun sequence.
ACCESSION   DACADA010000090 DACADA010000000
VERSION     DACADA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.092"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            153..1052
                     /gene="yegS"
                     /locus_tag="ISB17_002994"
     CDS             153..1052
                     /gene="yegS"
                     /locus_tag="ISB17_002994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAP1980895.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1134..1913)
                     /locus_tag="ISB17_002995"
     CDS             complement(1134..1913)
                     /locus_tag="ISB17_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1980896.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2013..3053)
                     /gene="gatD"
                     /locus_tag="ISB17_002996"
     CDS             complement(2013..3053)
                     /gene="gatD"
                     /locus_tag="ISB17_002996"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP1980897.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3101..4455)
                     /locus_tag="ISB17_002997"
                     /pseudo
     CDS             complement(3101..4455)
                     /locus_tag="ISB17_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(4459..4743)
                     /gene="gatB"
                     /locus_tag="ISB17_002998"
     CDS             complement(4459..4743)
                     /gene="gatB"
                     /locus_tag="ISB17_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAP1980898.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4774..5226)
                     /gene="gatA"
                     /locus_tag="ISB17_002999"
     CDS             complement(4774..5226)
                     /gene="gatA"
                     /locus_tag="ISB17_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAP1980899.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            complement(5236..6498)
                     /gene="gatZ"
                     /locus_tag="ISB17_003000"
     CDS             complement(5236..6498)
                     /gene="gatZ"
                     /locus_tag="ISB17_003000"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAP1980900.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6527..7381)
                     /gene="gatY"
                     /locus_tag="ISB17_003001"
     CDS             complement(6527..7381)
                     /gene="gatY"
                     /locus_tag="ISB17_003001"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAP1980901.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7608..8660)
                     /gene="fbaB"
                     /locus_tag="ISB17_003002"
     CDS             complement(7608..8660)
                     /gene="fbaB"
                     /locus_tag="ISB17_003002"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAP1980902.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEESSAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            8917..10194
                     /locus_tag="ISB17_003003"
     CDS             8917..10194
                     /locus_tag="ISB17_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1980903.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10191..11195
                     /locus_tag="ISB17_003004"
     CDS             10191..11195
                     /locus_tag="ISB17_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP1980904.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11192..12157
                     /locus_tag="ISB17_003005"
     CDS             11192..12157
                     /locus_tag="ISB17_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1980905.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12131..12877)
                     /locus_tag="ISB17_003006"
     CDS             complement(12131..12877)
                     /locus_tag="ISB17_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1980906.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(12929..13747)
                     /locus_tag="ISB17_003007"
     CDS             complement(12929..13747)
                     /locus_tag="ISB17_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAP1980907.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(13812..14612)
                     /gene="thiD"
                     /locus_tag="ISB17_003008"
     CDS             complement(13812..14612)
                     /gene="thiD"
                     /locus_tag="ISB17_003008"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP1980908.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14609..15397)
                     /gene="thiM"
                     /locus_tag="ISB17_003009"
     CDS             complement(14609..15397)
                     /gene="thiM"
                     /locus_tag="ISB17_003009"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAP1980909.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15432..15528)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15620..15892)
                     /gene="rcnR"
                     /locus_tag="ISB17_003010"
     CDS             complement(15620..15892)
                     /gene="rcnR"
                     /locus_tag="ISB17_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAP1980910.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16013..16837
                     /locus_tag="ISB17_003011"
     CDS             16013..16837
                     /locus_tag="ISB17_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAP1980911.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17056..17394
                     /gene="rcnB"
                     /locus_tag="ISB17_003012"
     CDS             17056..17394
                     /gene="rcnB"
                     /locus_tag="ISB17_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAP1980912.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17476..>17749)
                     /locus_tag="ISB17_003013"
     CDS             complement(17476..>17749)
                     /locus_tag="ISB17_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1980913.1"
                     /translation="DLLTNSNTILKGAEDNGVGFILESNGTPVTLLNITNSSKGYTNL
                     KEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         4350 a         4604 c         4295 g         4500 t
ORIGIN      
        1 ttcgctgtcc ccattgtaag gtcctggtgg taagcactga atccccatac ctgaactgac
       61 tttttatcct ccgactttca tcctgttctg actccacctt ttcttttctg ctctacacta
      121 tctacagacc aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga
      181 ttcttaatgg caaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg
      241 aggaaggaat gacgatccat gtgcggatca cctgggagaa aggcgatgct gcacgatttg
      301 tagaggaggc ccggaagtta ggcgtcgcaa cagtgattgc cggtggtggc gatggcacca
      361 ttaatgaagt ttctacggca ttgattcaat gtgaggggga tgacataccc gcgctgggaa
      421 ttttaccatt aggaactgcc aatgattttg ccactagtgt agggattcct gaggcactgg
      481 ataaggcgct gaaactggca attgccggta acgccattgc gatagatatg gcgcaggtca
      541 acaaacaaac ctgttttatt aatatggcga caggcgggtt tgggacgcgt attaccacag
      601 aaacgccgga aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa
      661 tgcgcatgga cactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc
      721 aaggtgacgc cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat
      781 tgtgcccgaa cgcattaatt aacgatggct tgctgcaact gcgcattttt accggcgatg
      841 aaattattcc gactctcgta tcaaccttaa aatctgacga agataacccg aatattatcg
      901 aaggtgcttc gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg
      961 gcgaaccgtt gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc
     1021 gattaccacc agattgtcca ttgttgcgtt aatcaaacta ttctctgttg aaccagagag
     1081 aatccgtaat gccggatacg gtgtaaacac cttatccggc atacagaaca atactacacc
     1141 gtaattaatt ttacccgtgt cttctgcaat gccagtttat ccacctcaga cacagcatca
     1201 tcggtaataa taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta
     1261 aatttcgatg aatcaagaac ggcaatcact ttattcgccg ctgtcaccat cgcaccgcta
     1321 acggaataac cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg
     1381 acaaacatta aatcggcatt aatatcctgc agggaacgtt cagcaataga tccatgcatc
     1441 gagcgcgttt tatggcggac cgtgccaccg cagacgacca gagtaatgtc tttattttcg
     1501 gaaagggcaa acgccgccgg gagactgttg gtaatcaccg tgatattttt ggcggtcatt
     1561 aatccttcgg cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttcg
     1621 tggatcattt tgactgccgc ctgcgctatt ctgtttttcg gcgcgctggc gagctgaaat
     1681 cgctctttaa acccgacttc ctgggtctcg gtttcgctat tacctgacat tattttcgcc
     1741 gcaccgccat gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg
     1801 attgtcgctt ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt
     1861 tcattcacta attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca
     1921 tattcaattg tggctagtgt aaacgaaggg gaaccattgg tgaatgatct gttttgcaca
     1981 ataccgggcc agcatcacgc tggcccgcgg tttcagggaa tgagcaacac tttgcccggc
     2041 atagcattac gagcgatgtc acgcaccgcc tgggcaaagc tttcaaagct tccacggtga
     2101 gcgattaatg gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac
     2161 tcctgccccg gccaagggct ggagtagttc atccaactgc cgataaccgt cagctcttta
     2221 cgcaatattt tgccaaacgt tgcagatgtt aaatgcagat cctgatgcaa cgtgcccacc
     2281 agcgccagtt gggcatgagg accggcaatc tctaccgcca gttcgacggt ttgcgggacg
     2341 ccagccgtct cgaggataag ctgattaaag cgcagctcgc gtaaaacgcc ctgtatttgc
     2401 ggcgcgctca tttcacggct gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc
     2461 agtttttctg agctaatgtc gatagccgtc acactctttg ctcccagcgc gacagcgcac
     2521 tgaattgcca gcaggccaat ggttccggca ccaataataa taacgttttt attctcacat
     2581 ccttgcgcta aatggaacgc atgcaggcca acggtaatcg gttcaataaa agcgccatcc
     2641 tcaataggca tatccgcagg tagtgcaaag acattgtttc gcttaacgac aatatattcg
     2701 gcaaatccac catcacgccg tgagccaata aaatcatatt ttgcgcactg ggaataaaac
     2761 cctttcagac actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg
     2821 ccggggtgta aatcatcaac accggatccc accgcatcaa tatagccact aaattcatgg
     2881 cctaacgtta ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca
     2941 cataagcccg agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg
     3001 acgctttctg ctacgcgcac gataccatca gtatcattca ccactgattt cataaaaact
     3061 cctgattgtt aagggggata acctccccct caggtaaaaa ttattctgcg agaacggctt
     3121 tctcttgttt aataaaacca cgcgctctac gccaggtcat gaaaataccg gtaagataaa
     3181 ttgcgccgat aacaatgaaa ccgggaatat tttgcgggga gaaaacctga atcagtaacc
     3241 aggtaatggg agaaccgccc tgatccattg aagccaccat acccccggct tttaacgcgc
     3301 ccgcattagc cgccagttgg gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga
     3361 tgctcataat gatgacaccc gagattaagg tgcggaacag atttccacga tgcaccgcga
     3421 ccgccatcgc cacaaagaag ccgatggtgg caaggtcgcc aaacggcagc acctgattac
     3481 ccggcacaca aacagcaatt aaaatggtga gtggaataaa aatcaggctt gccgatacca
     3541 ccgccgtatg tcccagcagc aacgccggat caaggccaat caggaactcc tgaccgccga
     3601 acttcgcctg taaacgacta cgggcctgct tagcgatggg cgttaaacca tccatgatgg
     3661 gtttaatcac ccgtggcatc agcagcataa ctgccgccgt ttttaccgcc agctgcaata
     3721 cacctttgac atcgtaaccc gcgaggatgc cgataatcag ccccatcaca aaacccacgg
     3781 tgaccggttc gccaaaaggg ccaaatttgc gctgaatatc atcggcgcta aatttaattc
     3841 ggttaacgcc tgggatttct cgatgatagc atcgaccagc acagcaattg gccccatata
     3901 cgccgacgta ccgtgcggaa tagcaatgcc ttccagctca aagaaatcac gggtatcgcg
     3961 ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac ccgccatccc
     4021 tatcatccat gaaccggttg ccagatgcag caacgcgccg gtgaaggtca tatgccagat
     4081 attccagata tcaacattta ccacccgcgt catacgggtc agtaacatcg ccacgttaac
     4141 cagaatcgca atcggaatcg ccaccagcgc aatttgcgac gcccaggtca tgggtgaaga
     4201 gcccggccag ccgacatcga ccacatgcag attcaggtcg aaattttccg ccatcgcttt
     4261 cgccgccggg ccaatggaat ccagcattaa gccaatcaca aggccaatgc caacaaagcc
     4321 aatcccgata tgcagacccg ctttaaagca atcgcctgcc ttcatgccta atattttaga
     4381 aaaaataatg atgacaatcg gcagcatcac cgtagggccg aggtcgagaa tataacgcat
     4441 gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt gtaatgcttc
     4501 gataccgaca ccagaaacaa aaggcatgcc gtgaactaac ggaatatcgc caaaactgcg
     4561 ttcaactttg gcggtagtac atatcaaatg cacgccatcc atataggttt ctatttcatt
     4621 aacccgacac tggattaatt caacaggaat attatgactc tgacacaact ctttaatttc
     4681 ttccgccgcc atcgtagagg tcgctaccgc gcctccgcaa gcgacaataa ttttgcgttt
     4741 cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta aaacatactt
     4801 tgtgaagtat tcctttaact ggggttcagg aagagtgatt agtgtctcga cgatatcggg
     4861 ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg gattttccac
     4921 aatcaacgca ataaccaacg ataccgccac gtcgttatca tcatccgctt gctgaaaaag
     4981 aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa tcgcctcaca
     5041 atgcggtatt gcaatggcgt gctgctcaag cattatcccg gtagggaatt ctgcttctct
     5101 ggcaattaat gcctgtggcc aggtatcatg aaccacacct ttcgcgagca tctcattacc
     5161 gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgccacgaa caaacaggtt
     5221 agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat
     5281 tttatccata ataagctgat gcggtattgc tgataattcc ccggactgaa tgcgttcaaa
     5341 ttgtttcgga agatactgac taatcatgcc cagtgggatg tctacgcctt ccagattcac
     5401 catcatcgtt tcgacgctat ttttaatccg gctatgcggc cagtaataac gaatacgatc
     5461 cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtgcgat aatatttctt
     5521 ccagtactgc ggttcgtcga gcatcacttc ttcaattacc gccaggcaac cactgctatt
     5581 ttcgggggcg ataagttcct gctcaatttg cgccagcgca aatatcgcct cgcgtaacgc
     5641 aaaggttaat gccggaccga ctttcaaaat tgcaaaatga tcgcgaacta attcccgata
     5701 ggccgcctga gtctggtaat cggtggaatg cgcttcataa accattttgg ttttttctat
     5761 ccattgcgcc aacgcctgag cttcttgcgc ctgataatga ataatattgc tatggtcaaa
     5821 ttccacacca ggctgcacca cgatggcaat cacgcgtgtt aacgcctctg ccagaccgcg
     5881 ggcaataaac gctttttgat gcgtgcgtaa agtattggcg gcatcttcaa cctgggtgat
     5941 gtgtactgac tgaatggcgc tggcctcacc gcccgggacc ggaacttcag tgccaatgac
     6001 atagttcagt tgctcacgct ggcaatcagt cgccacactt tccgccgcca gacaaagcac
     6061 agcggcacgt tcagcaaccg tttctggcgc taacggtatg gaatcgtcag cgcaggacat
     6121 tgacgcatca agatgaattt tactgaagcc ggcacgaaca tatgccttta ccagctcgac
     6181 ggatttttcc atcgccgcat ccgcattttc ttgctgccag cagtttggtc ccagatggtc
     6241 gccgccgaga ataatacgct cacgagcaaa tccgacttta tcggcaatag caaaaacaaa
     6301 ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga cctggtttga
     6361 cgttgcttca atcagcactt tacgcgtgct gttgcgatca aatgccagcg ccgcttcgat
     6421 aaccaacgga tgtgcagaac agactgaaca tatgccaata tgttcaccag ctttatgccg
     6481 ggcaattaac gttttcatgt tttttccttg ttaaatggca ggtgcgttat gccctgccct
     6541 cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcagat ttagccgact
     6601 gcaaataatc acggggatcg gtcgcttcgg ggtgctcggt caggtaattt tttaacgcct
     6661 gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc agtttgatgg
     6721 tttgctgaat atccttagtc gataatcctg acgcgccatg cagcaccagc ggtaaattca
     6781 cccactggcg aatgttctcc agtcgagaaa aatcaagcgc cggtgctctg gcatacatac
     6841 cgtgagccgt gccgatggcg accgccaggg aatcaatccc ggttgcctcg gcaaattcac
     6901 gcgcctgaac ggggttggtg tacaacgcat ctgcttcatt gacttgcaca tcatcttcct
     6961 ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagcgatgg caaaaatcca
     7021 ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg tcaatcatga
     7081 ctgagcgcac gccagaacga accttctgag cgatatcgtc aaattttgtg tgatggtcga
     7141 gatgaatcgc cagtggatgg tgatattgct tcgccatcgc gctgaccagc gccatcagat
     7201 tttctgtacc ggcatgagta aatgtgccgg gcgttccggc gatgatgacc ggcgcatgca
     7261 tgctggcagc ggtttccacc accacttgca tcgtttcgag attgtgaata ttgaatgccg
     7321 gaaccgcata accgccgcgc tgcgcgttgt tcagcatctg ctttgtcgat accacgtaca
     7381 ttttcacatc ctgtcatttg ttttcgattt caaaatataa tgaaattatt tgttttcaaa
     7441 tgtcgagata acgatcacaa aaacgacaat gtgaaaatta atcagaatga caggcaaaaa
     7501 aaacggcctc ccgggggaag ccgtaacgaa gtgcgcgtgt ttttatgccg gatgcaacgt
     7561 aaacgccttt tccgtcctac ggggtggttc tgttcgtatg aggcgcatca ggcgatagta
     7621 attttactat cgagataaac gtcctgtacg gcgttaatca gtttcacgcc gtcagccatc
     7681 gatttcttga acgccttacg tccaagaatc agccccattc cgcctgcgcg tttgttgata
     7741 accgcagtac gcactgcatc gctgaggtca gtttcaccgc ctgcagcacc gccggagttt
     7801 atcaacccgg cccggcccat atagcagtta gctaactgat aacgcaccag gtcaatcggg
     7861 ttttcactgg tcagtttgct gtagactcga tcgtcggtat aaccgtaatt aatcgcttta
     7921 tagccgccgt tattttccgc cattttttgt ttgacgatat ctgcacctat ggttgccgcc
     7981 agatggttcg cctgaccggt caggtcggcg gaaacatggt aatcaacgcc atctttctta
     8041 aaggcggagt tacgcaaata ggcccacagc actgtcacca tgcccagctc gtgcgcacgt
     8101 tcaaaagccg cagaactttc ttcaatctgg cgacgtgact cttccgagcc aaaatagata
     8161 gtcgcaccaa ccgccaccgc gcccatgttg aacgcctgct ccacgctggc atacagcgtt
     8221 tgatcgtagg tattcgggta acttagcgtc tcgttgtgat taagtttgac gaggaatgga
     8281 atgcgatgcg cgtaacgccg cgataccgat gccagcacgc cgtaagttga cgccacacag
     8341 ttacagcccg cttcgatcgc cagttcaaca atgtttttcg ggtcaaagta gagcgggtta
     8401 gcggcaaatg aagctccggc agagtgctca acgccctggt caaccggcag aatagaaaga
     8461 tatcctgtgc cagccagacg cccggtgttg tacaacgtct gcatattacg taacaccgct
     8521 ggcgggcgat tattgtcaat catcacgcgg tctacgtagt catgtccggg gagataaagc
     8581 tggtcagaag gaatagtcat acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc
     8641 aactgcgcaa tatctgtcat tactatgctc ccgtaaattc cgattggata tcggctatgg
     8701 attgtcctgg cccgcctttt gcgggcaatc ataatcctgg tcgttacggg cgagtttttc
     8761 catcttttgg atgttttttc agcgttttct gttggctcga ttcatcagaa aaaatgttac
     8821 cgcggtcaag ttttcaccgc aaaggtattt aaaaggtatt actaagtggt attgtcatcg
     8881 cgtaccttac attacctgtc atgaaggaat taaaagatga aaacaacagc aaagctgtcg
     8941 ttcatgatgt ttgttgaatg gtttatctgg ggcgcgtggt ttgtgccatt gtggttgtgg
     9001 ttaagtaaaa gcggttttag tgccggagaa attggctggt cgtatgcctg taccgccatt
     9061 gcggcgatcc tgtcgccaat tctggttggc tccatcactg accgcttttt ctcggcgcag
     9121 aaagtgctgg cggtattgat gttcgctggc gcggtactga tgtatttcgc tgcgcaacag
     9181 accacttttg ccgggttctt cccgttactg ctggcctact cgctaaccta tatgccgacc
     9241 attgcgctga ctaacagtat cgcttttgcc aacgtgccgg atgtggagcg tgatttccca
     9301 cgcattcgtg tgatgggcac tatcggctgg attgcctctg gtctggcatg tggattcttg
     9361 ccgcaaatgc tgggttatgc cgatatctca ccgactaaca tcccgctact gattaccgcc
     9421 ggaagttctg ctctgctcgg tgtgtttgcg tttttcctgc ccgacacgcc gccaaaaagc
     9481 accggcaaaa tggatattaa agtcatgctc ggcctggatg cgctgatcct gctgcgcgat
     9541 aaaaacttcc tcgtcttttt cttctgttca ttcctgtttg cgatgccact ggcgttctat
     9601 tacatctttg ccaacggtta tctgaccgaa gttggcatga aaaacgccac tggctggatg
     9661 acgctcggcc agttctctga aatcttcttt atgctggcat tgccgttttt cactaaacgc
     9721 tttggtatca aaaaggtatt gttgcttggt ctggtcaccg ctgcgatccg ctatggcttc
     9781 tttatttacg gtagtgcgga tgaatatttc acctacgcgt tactgttcct cggtattttg
     9841 ctgcacggcg taagttacga tttttactac gttaccgctt acatctatgt cgataaaaaa
     9901 gctcccgtgc atatgcgtac cgccgcgcag gggctgatca cgctctgctg ccagggcttc
     9961 ggcagtttgc tcggctatcg tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag
    10021 gaaccggtaa acggactgac tttcaactgg gccgggatgt ggactttcgg cgcggtgatg
    10081 attgccatta tcgccgtgct gttcatgatt tttttccgcg aatccgacaa cgaaattacg
    10141 gctatcaagg tcgatgatcg cgatattgcg ttgacacaag gggaagttaa atgaaaacag
    10201 aacgtattct cggtgctctt tatgggcagg cgttagggga tgcgatgggg atgccctccg
    10261 agctttggcc gcgcagtcgc gtcaaagcac actttggctg gattgaccgt tttctgcctg
    10321 ggccaaagga gaataacgcg gcctgttatt ttaaccgcgc cgaattcacc gacgatacct
    10381 cgatggcgct gtgtctggcg gatgcgttac tggaacgtga aggcaagatc gatccggatc
    10441 tgattgggcg taatattctc ggctgggcgc tgcgtttcga cgcctttaac aaaaacgtac
    10501 taggtccgac ctcgaagatt gcgcttaacg ccattcgcga cggtaaaccc gttgctgaac
    10561 tggaaaataa cggcgtgacc aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc
    10621 ttccggcgcg cgatgttgat tcctttattg atgatgtggc gctggcctcc agcccgaccc
    10681 ataaatccga tctggcggtt gcgggggcgg tagtcatcgc atgggcgatt tctcgtgcca
    10741 ttgacggaga aagctggtca gcgattgttg attcactgcc ttcaattgcg cgacatgctc
    10801 aacaaaaacg catcactacc ttcagcgcct cactggcagc acgtctggag attgcgctga
    10861 aaattgtgcg caatgccgat ggcaccgaat ccgccagcga acagctttac caggtcgttg
    10921 gcgcaggtac cagtactatt gagtccgttc cgtgcgccat tgcgctggtt gaactggcac
    10981 aaaccgaccc gaaccgttgc gccgtcctgt gcgctaacct tggcggcgac acagacacca
    11041 tcggtgctat ggcgacggca atttgcggcg cgttgcatgg cgttaacgct atcgatcctg
    11101 cgttaaaggc ggaactggat gcagtaaatc agcttgattt caaccgctat gccacagcgc
    11161 tggcgaaata tcgtcaacaa cgggaggcga tatgagcggc gctcgattac acacgctgct
    11221 gcctgaatta accacgcgtc agccagtgat ggtcgtcggc gcggcggtca ttgatgtgat
    11281 cgccgacgct tacgctctgc cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt
    11341 taacgttggc ggctgcgcac tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc
    11401 gggtaatgcc ctgccgctcg gtcagggcgt gtgggcggag attattcgca accggatggc
    11461 gaaagagggg ttaatcagcc tgatcgataa cgctgaaggc gataacggct ggtgtctggc
    11521 gctggttgag ccggatggcg aacgcacttt tatgtcattc agtggtgtgg aaaatcagtg
    11581 gaatcgcgaa tggctggcgc gattaaccgt tgcgcctggc agcctgctct atttttccgg
    11641 ttatcaactg gcctcgccct gcggcgaatt gttagtggaa tggctggaag agctgcaaga
    11701 cgtgacgcca tttatcgatt ttggcccacg tattggcgat atcccggatg cattactggc
    11761 gcggatcatg gcctgtcgac ctttagtgtc actcaatcgt caagaggctg agattgccgc
    11821 cgaacgtttt gctttatccg cagagataac aacacttggc aagcaatggc aggagaaatt
    11881 tgccgcgccg ttgatcgttc gtctcgataa agagggcgca tggtatttca gcaacgacgc
    11941 ttctggctgc attccggcat ttccaacgca agttgtagac accattgggg cgggcgacag
    12001 tcatgccggc ggcgtacttg ccgggctggc ctctggtctg ccactggcgg atgccgtatt
    12061 actgggcaat gcagtggcgt cgtgggttgt cgggcatcgg ggcggtgatt gtgcgccaac
    12121 gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc gctgcgacag tggctgatgc
    12181 tgtactcaat aggccgttgc tgttggtcaa gcgccacttg cttgatcacc agcaccggta
    12241 ttttgctatc taactgaata tgtgactgaa actcggcatc cggcatccgg gcgctaacgc
    12301 gggaacgcgt acgttgtggg taaatgtgct ggctgcggaa gtagtcataa agagaaatcc
    12361 cgatggcatc aacatcatga attaaatgtg ccggaaccca cgattcctca atcgataccg
    12421 cctcttcatc gacatagcga atacgcttaa gcaaaaaaac atcacttcct gcctcgaccg
    12481 ccagctgctg cgcgacgtct tcgggacatt tcacgacacg cttgttaacc catagcgtat
    12541 cgggcttttt cccgcgcaac accacctgct gagaaaaacc tcgcgcttct ttcaacgagt
    12601 attcgaagat gttgttgatc tgcgtgccgt aaccacgcga acgcgtcacc acgccctctt
    12661 cttccagcga ctgcatcgcc ttgcgcaccg taatgcgcga aacgccggtt aactgactta
    12721 aatcacgctc accgggcaaa atattgccat gctccagcac cccgctgcgc acggcatttt
    12781 ttaccgtttc ggcaaactta atataaagcg gcgtgttatc cgccgctaaa atacgctcgt
    12841 tcagttgggc aatcagctgg gtatgcgctt gttccatttg tctttttcct gccggggaat
    12901 ctggtgggag tatatcactg cttcttattt aaggcgtttc tttaatccca tcaacgaatc
    12961 cctgcaactc ctcctctgtg ccattaaaca cattaaaatc caccggacca ttaatgccat
    13021 cgacctgtcc acggtcggaa tgctgccaga agcgccaggc catgccgtca ttgtccgggc
    13081 gacgttgata atagtgagcc acccaccacg gatattcatt gaaatagccc gccagattgg
    13141 tgtgataaaa aacggctcct gagtaaataa tcggcttttt tcccgtacgt ttttcgacca
    13201 tttttagcca ctgacttacc cgcttgcgta attcttttgc cgataatttt cctcgctctt
    13261 ctacgtccag taccgcaggg aaatcgcctt gtgagaaatc caccgtttgc agaaataatc
    13321 gcgcctgaac tgaagcggat actgacgggg agaaataatg atacgccccg cgcagcaggc
    13381 cattttcccg gcttagttgc cagttacgcg aaaaataggg gtccaccagc ttttcgcctt
    13441 ccgtcgcctt aataaaagca aactgtaagc ggataccgtt gtcgcgcatt tttgccaccc
    13501 gctgccagtc gatccgctcc tgccagcgcg aaacatctat tccgtgaatg gtgtaactgg
    13561 caggaatgcg aatagcgaaa gatttaaccg ggcgataacc gtagaagtgg atgtagtctt
    13621 taatctgaat tgctgtcgaa tagaaaaaat tcaccgtctg acgcggataa attgcaacga
    13681 tggaaattag cccaagagcg cataataaaa cggtgaattt ttttcgactg gtgattctca
    13741 actgcattgg tttcatccct gaatgtcagc accaaaggct gacaataacc aaagcagcta
    13801 tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact
    13861 tccagcgtgt cggcctgggc taatgccgat gaaagccagc tttttgcctc ctgtacggtg
    13921 tcagcccagt ttgtatggcg cgggcgcagt gcagccaacg ccgcagagag cgtacaacca
    13981 gtgccgtgag tgtttttggt cataatgcgc tgtgcggtaa accgttgttc accctcgcgg
    14041 gtaaacagcc agtccgggct ttgctcatca tccagatgac cacctttcat tagcactgcg
    14101 ccacagccca tcgccaacag cgatcgccct tgttccagca tttcctgttc ggtgcgcgcg
    14161 tgtggcgcgt cgagcaaggc ggctgcttcg ggcaagtttg gcgttattaa tgaaacctgt
    14221 ggcaataatc gactgcgtag cgtggcaact gccgaaggtg aaagcagcgg gtcgccgctt
    14281 tttgccagca taacggtgtc gagtaccacg ttttggatct gataacgttg caaccgttct
    14341 gccaccgctt caacaatatc ggtttccgcc aacataccga ttttagtggt gtcgattcgc
    14401 acatcgctga acaccgaatc gagctgggcg gcgacaaaat caggctcaat gcgatacacc
    14461 gactgtacac cacgggtatt ttgcgccacc agtgcagtaa taactgagca accataagcg
    14521 ccaagtgccg agaaggtttt aagatcggcc tgaatccccg caccaccact cggatcagta
    14581 ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac ctcctgcgtc aattgccaga
    14641 gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc gcttctggcg actgcgcgtt
    14701 ctccggcttg tttcatccag tgacaggcag atgcgatatt ttccagcgta tcgcctggta
    14761 gcgcacaaca ggcagcgaca accgccgata gtgcacagcc agttcctacc actttggtca
    14821 ttaacggatc gccaccgtga atgccaatga tacgatgtcc atcggtaaca taatccacct
    14881 cgccagtgac cacgacgatt gcgccagttt cccgtgccag tgtttgtgca gcaggtatcg
    14941 cgttagctgc ggcatcagtg gtatccactc cccgtccacc attagcaacg ccagctgatg
    15001 ccatgatttc cgaagcatta ccacgtatgg cggttggctt atgggacaga agttccagac
    15061 aaaaacggcg gcgataatcg agcgcaccaa ccgcaaccgg atcaagcgtc cagggcgttt
    15121 gtgatcgtgt tgcttgctca acggcagcac tcatcgcttg cgctcgtggc cgcgtcagcg
    15181 tgccaacgtt aatcaacaag gcactggcga tagccgcaaa ctgactggcc tcttcggttt
    15241 cgataaccat cgctggcgat gcgccgagcg ccagcaaggt attggcagta aaagtttgca
    15301 ccacatcgtt ggtcatgcag tgcacaagag gggaatgttg gtgaaaaagg tgtaacgcgt
    15361 gcgcagattg cgctgaactc agcaggtcga cttgcatagt ttgctcctgc cataacgtga
    15421 agaagcaatg acctggtggt ccgtgacttc cctacgctgg cattatccag atcaggtgat
    15481 acgggtattt ctcagccttc acaaagaagg gcaccccgag tcgtttggtt gcgataataa
    15541 atcgcagagg aggatggtaa tgtccagcgc acgcgttgta aacggggaaa aacggcggta
    15601 ttacaataac gttgtgaaat tatttgatat aagaatccag caccttcaga acgacatcca
    15661 gatcttcttc acgttttagc tcatccccct ggtgaacgat gtgttccgtc agatggcctt
    15721 taatcacttc ccgcatcaga ccgtttaccg cgccacggat agcagcaatc tgttgtaaaa
    15781 ctgcagcgca ttcatgcggt tcgtcgagca ttttcttgag cgccacgacc tggccctgaa
    15841 tcttactggc acgcgctttc agtttctgtt tatcacggat tgtatgagac atggcaacac
    15901 ctggttaaca agaatatgaa aaatcatagc actattaatc tactgggggg tagtatcagg
    15961 tactgggggg gagtagaatc agattgccga attaatacta agaattatta tcatgaccga
    16021 atttacaaca cttcttcagc aaggaaacgc ctggttcttc atccccagcg ccatcttact
    16081 tggtgcgctt catggtctgg aaccggggca ctcaaaaacg atgatggcgg cgtttatcat
    16141 cgccatcaaa ggcaccatta aacaggcggt gatgctcgga ctggcagcaa ctatttcgca
    16201 taccgcagtg gtctggttaa ttgcctttgg cgggatggtg atcagcaaac gctttactgc
    16261 tcaatcagca gaaccgtggc tccagttgat ttccgcagtg atcattatta gcaccgcgtt
    16321 ctggatgttc tggcgtacct ggcgcggcga acgcaactgg ctggagaata tgcacgagca
    16381 tgatcatgag catcatcacc acgatcacga agatcaccac gaccatggac atcatcacca
    16441 tcacgaacat ggcgagtatc aggatgccca tgcacgagcc catgccaatg acattaaacg
    16501 acgctttgat ggtagagagg tcaccaactg gcaaattttg ctatttggct taaccggtgg
    16561 ccttatcccc tgcccggcag caattaccgt gctgttgatt tgcattcagt tgaaagccct
    16621 gacactgggc acaacactgg tcgtcagttt cagcctcggc ctggcgttaa cgcttgtcac
    16681 cgtaggcgtt ggcgcagcaa tcagcgttca gcaggtcgca aaacgctgga gcggatttaa
    16741 cactctcgct aaacgcgctc cctatttttc cagtctgttg attggcttag ttggtgtgta
    16801 tatgggcgta catggcttca tgggcataat gcgataactc aatcctttag gcttgcatgt
    16861 tatcttcagc ccaggatgat tcttaaatca gctatttccg ctgactctct gctcgctaag
    16921 gatgctttta gggcatcctt tcatttacac tttttacgaa atcatgggat cactaacaaa
    16981 atatcgcttg tcagttatat tgtatggcag gaaagatatg cgactgatat tacagatccc
    17041 caaagtggag agtttatgac cattaaaaat aagatgttgc tgggtgcgct tttgctggtt
    17101 accagtgccg cctgggccgc accagccacc gcgggttcga ccaatacctc gggaatttct
    17161 aagtatgagt taagcagttt cattgctgac tttaagcatt tcaaaccagg tgacaccgtc
    17221 ccagagatgt accgcaccga tgagtacaac attaagcagt ggcagttacg taacctgccc
    17281 gcgcctgatg ccgggacgca ctggacctat atgggtggcg cgtacgtgtt gatcagcgac
    17341 accgacggta aaatcattaa agcctacgac ggtgagattt tttatcatcg ctaaaaaaaa
    17401 gccccctcgg agagaggggg gaatgcagac accttaatat ttttattatt agccacttgc
    17461 tcgtcttgct tgttattagt cgtatttcac gttgattaat gcggttgcct ccagtgcgcc
    17521 agatttaact ttgtttgtat cgtagacgta gtaactggcc gttatcggaa ttgaaaccgt
    17581 tgtatctgta agttttgact ttgccgcaat ttcttttaaa tttgtatatc ctttactgct
    17641 gttagtgata tttaaaagtg tgactggcgt accattactt tcaagaataa atcctacgcc
    17701 attatcttcc gcacctttca ggatcgtgtt gctgttggtt aacaagtca
//
LOCUS       DACADA010000091        17730 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.093, whole genome shotgun sequence.
ACCESSION   DACADA010000091 DACADA010000000
VERSION     DACADA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.093"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(289..879)
                     /locus_tag="ISB17_003014"
     CDS             complement(289..879)
                     /locus_tag="ISB17_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980914.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(929..2125)
                     /gene="hemY"
                     /locus_tag="ISB17_003015"
     CDS             complement(929..2125)
                     /gene="hemY"
                     /locus_tag="ISB17_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAP1980915.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2128..3327)
                     /gene="hemX"
                     /locus_tag="ISB17_003016"
     CDS             complement(2128..3327)
                     /gene="hemX"
                     /locus_tag="ISB17_003016"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP1980916.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(3349..4089)
                     /gene="hemD"
                     /locus_tag="ISB17_003017"
     CDS             complement(3349..4089)
                     /gene="hemD"
                     /locus_tag="ISB17_003017"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAP1980917.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4086..5027)
                     /gene="hemC"
                     /locus_tag="ISB17_003018"
     CDS             complement(4086..5027)
                     /gene="hemC"
                     /locus_tag="ISB17_003018"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAP1980918.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5414..7960
                     /gene="cyaA"
                     /locus_tag="ISB17_003019"
     CDS             5414..7960
                     /gene="cyaA"
                     /locus_tag="ISB17_003019"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAP1980919.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(8000..8320)
                     /gene="cyaY"
                     /locus_tag="ISB17_003020"
     CDS             complement(8000..8320)
                     /gene="cyaY"
                     /locus_tag="ISB17_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAP1980920.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8368..8631)
                     /locus_tag="ISB17_003021"
     CDS             complement(8368..8631)
                     /locus_tag="ISB17_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980921.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQK
                     QGKGVVYLRPYEKTNLSLYRDIIIPVTDESCRNAAADGQTLKP"
     gene            8790..8993
                     /locus_tag="ISB17_003022"
     CDS             8790..8993
                     /locus_tag="ISB17_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1980922.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9030..9854
                     /gene="dapF"
                     /locus_tag="ISB17_003023"
     CDS             9030..9854
                     /gene="dapF"
                     /locus_tag="ISB17_003023"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP1980923.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGIGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9851..10558
                     /locus_tag="ISB17_003024"
     CDS             9851..10558
                     /locus_tag="ISB17_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAP1980924.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10555..11451
                     /gene="xerC"
                     /locus_tag="ISB17_003025"
     CDS             10555..11451
                     /gene="xerC"
                     /locus_tag="ISB17_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP1980925.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11451..12167
                     /gene="yigB"
                     /locus_tag="ISB17_003026"
     CDS             11451..12167
                     /gene="yigB"
                     /locus_tag="ISB17_003026"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAP1980926.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12251..14413
                     /gene="uvrD"
                     /locus_tag="ISB17_003027"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12251..14413
                     /gene="uvrD"
                     /locus_tag="ISB17_003027"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAP1980927.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14468..15370)
                     /locus_tag="ISB17_003028"
     CDS             complement(14468..15370)
                     /locus_tag="ISB17_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HAP1980928.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15453..16217)
                     /locus_tag="ISB17_003029"
     CDS             complement(15453..16217)
                     /locus_tag="ISB17_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HAP1980929.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16587..17537
                     /gene="corA"
                     /locus_tag="ISB17_003030"
     CDS             16587..17537
                     /gene="corA"
                     /locus_tag="ISB17_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAP1980930.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4082 a         4644 c         4716 g         4288 t
ORIGIN      
        1 aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg ttatttcccg
       61 gttctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt cgccttagtg
      121 cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca ctgacagatg
      181 tcgcttctgc ctcatcagac accatggaca caacgttgag tgaagcaccc acttgttgtc
      241 atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt atgaccgagt
      301 aaacaacagg ctacgtcgct cttgggtcat cggttgtgcg tttaatacac ttacaagaga
      361 acctaccgtg gtgatgggaa gaaacggatt atcttctatt ccgcgtagat cgcgcccaca
      421 acgagcggca atatcttcga ccagctcttc taactcgtct gcatcgttta ctaaatccag
      481 atcatcgtcc agtttatcac tggctttcag tggtaatgga taatcgatat agccgtttcc
      541 ttgtaaggta ttccacacag cccgaatcac ccatgtatct acggtgtggg gggaaaattg
      601 gcgtgcgaac tgacaaatgg aaagtccctg acgtttatca gccataatct ggcgcttctc
      661 gatgagcttg attttttcct gcttcgcaat ggctatcaga tacccaataa aaagtgttaa
      721 ccagaagatg aatgtattgc ctgtacgtac gaaggacaga atcagccaac ttacgccagc
      781 cagaacggca catatcaaca aaatcagcaa gagcgttgcc cgaaaaggat taatttcatg
      841 tgatggtaag cggaaaatat agcgggacgg tgtgcgcatg tttgacatcc ttgtaaaaaa
      901 atatcgcccc tgaacgaggc gatatagatt actgcggtgg gttgttttgc aacgttagca
      961 tcaaaccgtc gcgacgcatc gctgcggctt cttccggctt gtgcagtctg tccagcgcgt
     1021 cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt cagcgctgcg cggaaggcga
     1081 gcgatgcttc ctgccattct ccgtgtttca tcagcgactg acccagtgtg ctccacaaca
     1141 gcgggcgatc gcccacgttt ttgatctgtt ggcgcagcac tttttccagc tgttccggat
     1201 tattggtttt cagacgcggg atcggcagca gcaggcgatc gtcatactgg cgtttcaggc
     1261 cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca ctcaataaga tgttccgcca
     1321 ttgccacctg caaggccacc tgatgacgcg ttttccggct ttggtttttc caccagttac
     1381 gcaaaccttc gctaccgtta tcggcacgcg cctgatccat caggccaatc catgcctgtt
     1441 gttccagcat tgcacgatgt tcttcatcac caacatgggc tttcgccatt gatgggatga
     1501 tatccagcag cgaactccat gcccctgtgc ggatatacgc ctgttccgcc agacgtaata
     1561 cttccggatg gcgtggcgta acttccagca gcttatcaac gccgtgacgt gctgcatggt
     1621 tttcattacg ggccagttgc agacgtacac gggtgatttc taccggaatg gtgtcattgc
     1681 cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg tgcttcatca ccacgttgtt
     1741 gcgcggcttc ggctgccagt agatagttca ccaccggttg ttcagcgtga tcggcatttt
     1801 tcgccatcag cttttcaact tgctgataat cgccttccgc cagtttcagc agcgcctgtt
     1861 cggtctgctt acgtgcacgg cgacgcttac gtccgacaaa ccacccacgg gtgtgcgcgc
     1921 ctgtgcggaa gattcgccgc agtagccact caatggcaaa cagcactacc atcgccagaa
     1981 tcaaaataat cgcgaggccc gtgacgctag tttcaatatt gtagttgtcg gtctggatca
     2041 gcacgtaacc ctgatggccg gcaatcatcg ggccaaccac gatccccgca atcagcaaca
     2101 caaagagcaa taacactttt agcatggtta ttctccttgc ggcgcggctg ccggagcatc
     2161 agcttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccctgc
     2221 tgccggttgt gccagcaggt tacgcacgcg ggtctgcatc aatttttcca gcatcgcctg
     2281 gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc
     2341 gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga
     2401 gacgttctcc agcgcctggc gataagtctc ttcctggtga cgcggtacag cttgtgctgc
     2461 gaccagcagg cgagagcgga tattttcgcg cagatagata tcctgatttg gcgctaacag
     2521 cggtacggca gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa aattctgcca
     2581 gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt caccgtcgga
     2641 atccatcggc gaaccatcgc tgtcattatc ggccagacgc agattatcta cctgatttga
     2701 aagctgatta agcttaagga tgataccgtc ataatccacc tgcgataccg cagaaaggct
     2761 ggcgatatca tcggtaattg cccggcgaac ggtaatcaga ctcgggtcat tcatatccgc
     2821 caggctggcg tctgcacttt tcagcaacgc tgcagcggtc gtgacgtcct gatcgctcca
     2881 cagcttccgt ccggcgagtt tcaccagaaa atcggcctgg gccagcagcc aggttttagc
     2941 atcgctgccg gaaatggtgg cgaccttttg ttggacttca tccagctgtt ttgccagcgt
     3001 ttcttgctga cgattcgcct gctcaagctg tacagcttgt tgcttgataa tgccttccag
     3061 ctcggctttt tggctctcct gggctttttg cagtgccgta agttggttag ccagggcatc
     3121 gctggtggca gtctgattga cggcctgttg tttaccccag ccatacaaac cgacgcccgc
     3181 cgccagagca atagcgatag ccaccgcgct gagaatcaat gcggtattgt tcttactctt
     3241 tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac
     3301 ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc
     3361 ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc agcccagttc
     3421 ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagcgta gtaaccagtg
     3481 ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac
     3541 aacgaccgtc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg
     3601 gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgcg ccgtcaggga
     3661 atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacatt tgcccgcaat
     3721 attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gtgggtagag
     3781 aatcttctgt ccgcttacgg tatgtagtgc cagcgcggtg gtgcgtccaa tggcgaaata
     3841 agtaggtagt cggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac
     3901 cgcgtgttgc gagagggcaa acaacagatc gctctctccc agcgctgcca gttgatcagc
     3961 aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg
     4021 ccccagtgtg cgcagacggc tcactaactc ttctcctgcg ggagacgggc gggtgaccag
     4081 gatactcatg ccggggcgtc tccgttatag acttcagcga ggatctcgcg cgcgccgtta
     4141 ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg
     4201 cgttcaccgc gaataatcag cgaaccgtcc ggcgcgccga ccaacgcacg cagccagatt
     4261 tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg
     4321 gtattcatgg cgcgttcggc ggtaacacgc agtgcagttt cgtggtgatt cagcgcggcg
     4381 agcagctcgc gagtgcgtgc atcatcaagg cggcattcaa tgcccaccgc accttgccct
     4441 accgccggaa gagaaatctc gggtggcaac gcggcgcgaa tgcgggactc cagacctaaa
     4501 cgttttagtc cggctacggc aagaatgatg gcatcgtatt caccgttatc cagtttgctc
     4561 aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc
     4621 agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgccgg taacgcatcc
     4681 agactgtcat agttattgga cacaaatgca tcgcgaggat cttcacgctc gcaaatagtg
     4741 accagcccca gaccttgcgg gaattcaacc ggcacatctt tcatcgaatg tacggcgata
     4801 tcggcgcgat tttcgaggag cgcgacttcc agctctttaa caaataagcc ttttccgcct
     4861 actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca
     4921 acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt
     4981 gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc
     5041 attatcatcc gtggtccatc ctaacatcct tgccagagcg atgtcagtgt tgtggtgaaa
     5101 cgtagacgcc tgcgcaaacc gtaaaatgag gtcgggcagt ggatcctgac aggcgctttg
     5161 agccttcgta ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc
     5221 tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc
     5281 gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat
     5341 cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat
     5401 caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa
     5461 tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag
     5521 tctactgccg acattgttgc actatcacca tccgctaatg ccgggttacc ttgatggtaa
     5581 cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga
     5641 gcttgaactg tatcgtggaa tgtcagtaca ggacccaccg aaaggtgagc ttccaattac
     5701 tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga
     5761 tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttgc tacaacgtaa
     5821 atgtagtctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat
     5881 tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc
     5941 cacccagcat atactgctgc ttgacgaatt ttatcgtacc gccgtgcgtc tcgccggtaa
     6001 gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat
     6061 gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatcttg gtggcttaag
     6121 ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga
     6181 ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc
     6241 gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg agattgtatc
     6301 gtttggcctc gacccgtact gcatgatgct ggagcgtgtt actgaatacc tgacggcgat
     6361 tgaagatttc acccgtctgg atttagtgcg tcgctgcttc tatttaaaag tgtgcgaaaa
     6421 gctcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag
     6481 cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgcgcta actggaagat
     6541 agatcaggtg cgtgaggcgc acaacgagct gctcgacgca atgatgcaga gctaccgtaa
     6601 tctgatccgc tttgcgcgcc gcaataacct tagcgtctcc gccagcccgc aggatatcgg
     6661 cgtgctgaca cgtaaactgt acgccgcgtt tgaagcatta ccgggtaaag tgacgctggt
     6721 aaacccgcag atttcgcccg atctctcgga accgaatctg acctttattt atgtaccgcc
     6781 gggccgcgcc aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcaat
     6841 catcagccat cagccgctgg aatataatcg ctacctgaat aaactggtgg cgtgggcatg
     6901 gtttaacggc ctgctgacct ctcgcacccg tttgtatatt aaaggtaacg gcattgtcga
     6961 tttgcctaag ttgcaggaga tggtcgcaga cgtgtcgcac catttcccgc tgcgcttacc
     7021 tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc cgccatctgg cgattatcgt
     7081 taacctggaa tatgacccga cggcagcctt ccgcaatcag gtggtgcatt ttgatttccg
     7141 taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtgggta gcgttgacct
     7201 gctgtatcgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat
     7261 gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac cgccagatag
     7321 cgtggaagtc ttctgttata gccagcatct gcgcgggtta atccgtactc gcgtgcagca
     7381 actggtttct gagtgtattg aactgcgtct ttccagcacc cgccaggaaa ccgggcgctt
     7441 caaggcgctg cgcgtctccg gtcaaacctg gggcttgttc ttcgaacggc tgaatgtatc
     7501 ggtacagaaa ctggaaaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca
     7561 cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt
     7621 tgccagcgaa gggatcatcc agttcttctt cgaagaaacg caagacgaga atggctttaa
     7681 tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa
     7741 agaggaactg gtgcgtgacg tcagtcgctt ctactcgtct tcgcacgacc gtttcaccta
     7801 cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg
     7861 tcgtgaacag gtgattccgt ttcgcaccaa atctatcggt aacatgccac ctgccaatca
     7921 ggagcatgac gcgccgctgt tgcagcagta tttttcttga tgctaatgcc ggaaagcgag
     7981 gcttatccgg cattagctct taacgaaaac tgacggtttc tcccgcctgc tgcgtcgccg
     8041 cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccac tcatcgcctt
     8101 tcagatcaaa atggtagccg ccctgtttgg ttgccagcca tacctggtgc agtggttcct
     8161 ggcggttgat aatgattttg ctgccgttct caaaggtaat ggttagtaca ccgccgttga
     8221 tttcacagtc gatatcgctg tcgccatccc agtcgtccag acgttcttca atggtcagcc
     8281 acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag
     8341 tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcccgt ctgctgctgc
     8401 gttccggcat gattcatccg tcaccggaat aataatatct ctatatagcg aaagattggt
     8461 cttttcataa gggcgcaaat acaccacgcc ttttccctgt ttctggtaaa tattatgttc
     8521 cggctcatca agccgacatc cccatgccga tgataagaac aacgaacaag acaataaaca
     8581 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc
     8641 agcaaaacat aaatcgccac gagtaagcgt tatactcgca gcatttcctc acttttcaga
     8701 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
     8761 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
     8821 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
     8881 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
     8941 cggacaaaaa cgaccgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
     9001 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
     9061 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
     9121 gtcgcctggc ggatcggcac ctggggatag ggtttgacca gctgctggtg gttgagccac
     9181 cgtatgatcc ggaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtag
     9241 cgcagtgcgg caacggtgcg cgctgctttg cccgttttgt gcgcctgaaa gggctgacca
     9301 ataagcgcga tatccgcgtc agcaccgcca acggacggat ggtgttgacc gtcaccgatg
     9361 atgatctggt ccgcgtcaat atgggcgaac ccaacttcga accttctgcc gtgccatttc
     9421 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
     9481 gcgtggtgtc gatgggaaat ccgcactgcg tgattcaggt tgatgatgtt gatactgcgg
     9541 cggtagaaac gcttggtcct gttctggaaa gccacgaacg ttttccggag cgcgccaata
     9601 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
     9661 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
     9721 aaggtttgct ggccgaagaa gtacgcgtgg aactccctgg cggtcgtctt gatatcgcct
     9781 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
     9841 ttattcatct atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga
     9901 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
     9961 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    10021 catggcccgc gcacgtaatc atattcatgt tctggaagag aacatggcgt tgttgatgga
    10081 acaggctatc gccaacgaag gcctgtttta tcgtctactc tacctgcagc gcagtctcac
    10141 cgccgccagc agtctcgatg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    10201 cctggcaggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    10261 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtctggg
    10321 gcaggaacag cactatctgg gaccgcttaa cggaccggag ctgctggtgg tgctaccgga
    10381 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggggt
    10441 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    10501 tcatgaaatt gcgctgatgt tgccggagct tctggcgcgt tggattgaac gcgtatgacc
    10561 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg ccagcttagc
    10621 ccgataaccc tgcttaacta ccagcgacag cttgaggcga tcatccattt tgccagcgaa
    10681 aacggcctgc aaagctggca gcaatgtgat gtgacgatgg tgcgtaattt tgctgtacgc
    10741 agtcgccgta aagggctggg agctgcaagt ctggcgttac ggctttctgc gctacgtagc
    10801 ttttttgact ggctggtcag ccagaatgaa ctcaaagcta acccggcgaa aggtgtttcg
    10861 gcaccgaaag cgccgcgtca tctgccgaaa aacatcgacg tcgatgatat gaatcggctg
    10921 ctggatattg atatcaacga tcccctcgcc gtgcgcgatc gggcaatgct ggaagtgatg
    10981 tacggcgcgg gtctgcgtct ttccgagctg gtggggctcg atatcaaaca cctcgacctg
    11041 gagtctggcg aagtgtgggt gatgggaaaa ggcagcaaag agcgccgcct gccgattggt
    11101 cgcaacgccg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    11161 gacgacgcgc tttttctgtc gaaactgggc aaacgcatct ccgcgcgtaa tgtgcagaaa
    11221 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc gcataaatta
    11281 cgtcactcgt tcgctaccca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    11341 ctgctgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    11401 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    11461 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    11521 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    11581 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    11641 cggaacctga gatttatcac gacgtgacgc gctggcgttt tcgttcgatt gaacaagcga
    11701 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacatgca gcaatgatca
    11761 actttgccaa atggcgcagc cggatcgacg tcccgcagca aacccacgac accttaaaac
    11821 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    11881 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    11941 cgttcagcga tatgtacttc ctggcggcgg aaaaactcaa cgtgccgatc ggcgagatct
    12001 tacatgtcgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    12061 cctgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    12121 cgcatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    12181 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    12241 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac agcgcgaagc
    12301 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    12361 cgtactggtg catcgtatcg cctggttgat gagcgtggaa aactgctcgc cgtattcgat
    12421 tatggcggtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcgggcaact
    12481 gatgggcact agccagggcg gtatgtgggt cggcaccttc cacgggctgg cgcaccgcct
    12541 gctgcgtgcg caccatatgg acgccaacct gccgcaggat ttccagatcc tcgacagtga
    12601 agaccagcta cgtctgctta agcgtttgat caaggcgatg aatctcgacg agaagcagtg
    12661 gccgccgcgt caggcaatgt ggtacatcaa cagccagaaa gatgaggggc tgcgtccgca
    12721 tcatattcag agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    12781 tcaggaagcg tgtgaccgcg cgggcctggt ggacttcgcc gagctgctgc tacgtgctca
    12841 cgagttgtgg cttaacaagc cgcatatcct tcagcactat cgcgaacggt ttaccaatat
    12901 cctggtggac gaattccagg ataccaacaa cattcagtat gcgtggatcc gcctgctggc
    12961 gggcgatacc ggcaaagtga tgatcgttgg cgatgacgat cagtcaatct acggctggcg
    13021 cggagcgcag gtggagaata ttcagcgttt cctcaatgat ttccccggtg ctgaaactat
    13081 ccgtctggag cagaactacc gctctaccag caatattctg agcgccgcga acgccctgat
    13141 tgaaaacaac aacgggcgtt tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    13201 gatttccctt tattgcgctt ttaacgaact cgacgaagcg cgttttgtgg ttaatcgcat
    13261 caaaacctgg caggacaacg gcggggcgct cgctgagtgc gccattctct accgcagcaa
    13321 cgcccagtcg cgtgtgctgg aagaggcgtt attgcaggcg agtatgccgt atcgtattta
    13381 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    13441 gattgccaac cgcaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    13501 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgagatcgcc agttaacact
    13561 ctggcaggca tgtcgtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    13621 cttacagcgg tttatggagc tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    13681 gcatgtacag actgaccggg taattaaaga ctccggcctg cgcaccatgt acgagcagga
    13741 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    13801 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcat tcctctccca
    13861 tgcggcgctg gaagcgggcg aggggcaggc ggatacctgg caggacgcgg tgcagttgat
    13921 gacgctacac tcggcgaaag gtctggaatt cccgcaggtg tttatcgttg gtatggaaga
    13981 gggcatgttc ccaagccaga tgtcgctaga tgaaggcggg cgtctggaag aagaacgccg
    14041 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    14101 ccgccgtcta tatggcaaag aggtttacca tcgcccgtcg cgctttatcg gtgagttgcc
    14161 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    14221 gcgtatgggg acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgcgtacg
    14281 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggcg agcatagccg
    14341 tttgcaggtg gcattccagg gccagggtat taaatggctg gtggcggctt acgcccgact
    14401 ggagacggtg taatttacga ttccgttgcc ggatgcggtg cgctacaccg catccggcat
    14461 cagcacctta cttagcgcac ttcttcacat caatattcca gtcagcacca gacgcaacct
    14521 gatgtttctg cgcgaaatta tccatattca gcgccacgga aacattcaat aagctgttca
    14581 gatacaccat cggctggcct tccggcacat cgccgaagct tgcgacatac ggcattttcc
    14641 ctgcgtattt ttgctgggaa ccttcggaaa tggtcacaca caacgtgtcg ttgagtttga
    14701 ttcctgcctg attcagcaac tcgtcgctga tattgctcca gacgttgccg tactgaatat
    14761 cgagaatcgg gatattgccc ttcacttcac cttttgttgc tttggctttc tgataagaaa
    14821 gttcgacaac ttttgcagga agctctggcc ccacctgctc gaacgtgatc gccccggaag
    14881 ccagacgcgc accggtgtag gcatatacat cacgaccatg gaaggtatat gacttttcgg
    14941 aacctttcag acggttggtt ttttcatcga tttgccggac gctctcaata cccagcgatt
    15001 ccgccaccaa cgtgagcgta ccgttatctg gtgaaacgaa gtactgaccg tttttggttt
    15061 tcagcactac cgatttacgg tctgtaccaa cacctggatc gaccacgcta acaaacaccg
    15121 aaccttgcgg ccagtaactg gcagtctgat acaggcgata agcgccttcc cagatgttat
    15181 acggtggaat ttcgtgtgtt aaatcaaaga ttttaagatt atggtcaaca ccgaatgcga
    15241 cccctttcat cgccgagacg gcaccatctt tcaggctaaa atccgtttgt aaaatcaaag
    15301 cgttatccgc ataagcggat gatgacccca gacaggttaa taaaaataat gttgcgagcg
    15361 tttttttcat ttttgtttcc cttcatttat ctgatgattt tccagctgtg tagccagccc
    15421 cagtttgtct ggcctcctcg agtgtacaat gtttaacctt tctgctcgac ggaaatcatg
    15481 gtgacaaaag gatagcgttg ccacgggatt gcctcgcctt tcatatacat atgtgaaatt
    15541 gtgccatcaa gataaagcag ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa
    15601 tcataaaaat ttgtcgcctg ctggctcagc aaaaacacgg cgttcccatg tttattaatc
    15661 ccaacaccgt tacgaatttt acgtgaggcg acgttgggat gaatacgcgg attaattaca
    15721 ccgttttcca tcaacaatgg ccctgactgc accgcaaact gaatctcttt actggtttta
    15781 aaggcatcca gacgaacgat gccgacttta tctcccgcga cataaaacac gccgccaggg
    15841 cgaataaaga aattcccttc acctgaagcg agatttaacg ccaccttctg ctgaccgttt
    15901 tcgatgtaca aaccgagcgg cgcatagctt tcatcataga tgccgccgtt catcgccatc
    15961 tgcacctgac cctgactatt aatatccgcc agcagcgcat gtaacgttcc ccacgcttcg
    16021 ccattggctt tttgccagta cattttcacc cgctctgttt gaggattaac ggtatacgcc
    16081 tgtacggtca gcgtcggatc tgagggtgcg caatcatcag cggcaacagc aaacaaggga
    16141 agtaaggtga gggtgagaaa aatccgtttg agattcaagg tgatcattcc tttaccaatg
    16201 aatagccgat gcgccattat aggccctgaa tgtgggattt ttttatcctg ttagcgacct
    16261 tgacgagtac caaaaagcgc gaagttcaac tattgttctg tggtgttctg ttgcgtgttg
    16321 acggcaaaat tttgctggcg taacatgcgc gcacgatcac tctaagagga cattcgcctt
    16381 ggacacaccc agtagatact ggctcactat cctgtcatcc aggatcaact cctaaggcta
    16441 tccctttttg ctgatagcct tagcggttgt cagcgacctc aatttttccc gtcgcgctga
    16501 gtcaggctgt ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg aactgtccga
    16561 tattttaagc attgggagtc ccggtcatgc tgagcgcatt tcaactggaa aataaccgac
    16621 tgacccggct ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg attgatcttg
    16681 tcgaaccgga cgacgacgag cgactgcgcg tacaatctga acttggccag agcctggcaa
    16741 cccgcccgga actggaagac atcgaagcat cggcacgttt ctttgaagac gatgacggcc
    16801 tgcatattca ctccttcttc ttctttgaag atgcggaaga tcacgccggt aactccactg
    16861 tggcatttac catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa ctgcccgctt
    16921 ttcgtctgta tcgtatgcgt gcccgtagcc agtcaatggt ggacggtaac gcctacgagc
    16981 tgctgctgga tctgttcgaa accaaaatcg aacagttggc agatgaaatt gaaaatatct
    17041 acagcgacct ggagcagttg agccgggtaa ttatggaagg gcatcagggc gatgagtacg
    17101 acgaggcgct ctccacgctg gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc
    17161 tgatggatac ccagcgcgcg ctcaacttcc tggtacgtaa agcacgctta ccgggtgggc
    17221 aactggagca ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg cataacgaat
    17281 ccctgttcca gaaggtgaac ttcctgatgc aggcggcgat gggctttatc aatatcgagc
    17341 agaaccgcat catcaaaatc ttctcggtgg tatccgtggt gttcctgcca ccgacgctgg
    17401 tcgcttccag ctacgggatg aactttgagt ttatgccgga actgaagtgg agcttcggct
    17461 acccaggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc
    17521 ggaagaactg gctgtaaaaa acgagaacgg tggcttagcc tggctaagcc accgattatt
    17581 taaaggctcc aggtatgaat accttttact tctttttcat agaaccatct gttggtattg
    17641 tatgaaacat aagcggcgat aatgaatcca aatacattat agccaatcat gtaaccaaaa
    17701 attaaagaaa atatcgccag gaatagaaca
//
LOCUS       DACADA010000092        17450 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.094, whole genome shotgun sequence.
ACCESSION   DACADA010000092 DACADA010000000
VERSION     DACADA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.094"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1080
                     /locus_tag="ISB17_003031"
     CDS             <1..1080
                     /locus_tag="ISB17_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1980931.1"
                     /translation="IYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSL
                     ALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHT
                     ATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNY
                     TIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLL
                     SRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEV
                     FGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISL
                     VMTEADKALREAKSEGGNKVIIHHI"
     gene            1261..2751
                     /locus_tag="ISB17_003032"
     CDS             1261..2751
                     /locus_tag="ISB17_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1980932.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2794..3297
                     /gene="yeaK"
                     /locus_tag="ISB17_003033"
     CDS             2794..3297
                     /gene="yeaK"
                     /locus_tag="ISB17_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAP1980933.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3298..3402)
                     /locus_tag="ISB17_003034"
     CDS             complement(3298..3402)
                     /locus_tag="ISB17_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1980934.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3572..4018
                     /locus_tag="ISB17_003035"
     CDS             3572..4018
                     /locus_tag="ISB17_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP1980935.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3975..4796)
                     /locus_tag="ISB17_003036"
     CDS             complement(3975..4796)
                     /locus_tag="ISB17_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1980936.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPGSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4893..6074
                     /gene="nimT"
                     /locus_tag="ISB17_003037"
     CDS             4893..6074
                     /gene="nimT"
                     /locus_tag="ISB17_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAP1980937.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6129..6476
                     /locus_tag="ISB17_003038"
     CDS             6129..6476
                     /locus_tag="ISB17_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAP1980938.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6498..6752)
                     /gene="yoaF"
                     /locus_tag="ISB17_003039"
     CDS             complement(6498..6752)
                     /gene="yoaF"
                     /locus_tag="ISB17_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAP1980939.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6935..7960
                     /gene="dgcP"
                     /locus_tag="ISB17_003040"
     CDS             6935..7960
                     /gene="dgcP"
                     /locus_tag="ISB17_003040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAP1980940.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            7993..8091
                     /gene="yoaK"
                     /locus_tag="ISB17_003041"
     CDS             7993..8091
                     /gene="yoaK"
                     /locus_tag="ISB17_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAP1980941.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8094..8168
                     /gene="yoaJ"
                     /locus_tag="ISB17_003042"
     CDS             8094..8168
                     /gene="yoaJ"
                     /locus_tag="ISB17_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAP1980942.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8227..8475)
                     /locus_tag="ISB17_003043"
     CDS             complement(8227..8475)
                     /locus_tag="ISB17_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP1980943.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8623..8805)
                     /locus_tag="ISB17_003044"
     CDS             complement(8623..8805)
                     /locus_tag="ISB17_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAP1980944.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8809..9168)
                     /gene="yeaR"
                     /locus_tag="ISB17_003045"
     CDS             complement(8809..9168)
                     /gene="yeaR"
                     /locus_tag="ISB17_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HAP1980945.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9341..9979)
                     /gene="leuE"
                     /locus_tag="ISB17_003046"
     CDS             complement(9341..9979)
                     /gene="leuE"
                     /locus_tag="ISB17_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAP1980946.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10106..10273)
                     /locus_tag="ISB17_003047"
                     /pseudo
     CDS             complement(10106..10273)
                     /locus_tag="ISB17_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10262..10432
                     /locus_tag="ISB17_003048"
     CDS             10262..10432
                     /locus_tag="ISB17_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP1980947.1"
                     /translation="MTTGGKQPGDIDFYVVRKNTEGEYSSPGGRVNEGTENEVVIQES
                     VFTRRGVERIWR"
     gene            complement(10522..11637)
                     /gene="rnd"
                     /locus_tag="ISB17_003049"
     CDS             complement(10522..11637)
                     /gene="rnd"
                     /locus_tag="ISB17_003049"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAP1980948.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11719..13404)
                     /gene="fadD"
                     /locus_tag="ISB17_003050"
     CDS             complement(11719..13404)
                     /gene="fadD"
                     /locus_tag="ISB17_003050"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAP1980949.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13609..14190)
                     /gene="yeaY"
                     /locus_tag="ISB17_003051"
     CDS             complement(13609..14190)
                     /gene="yeaY"
                     /locus_tag="ISB17_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAP1980950.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14230..14925)
                     /gene="tsaB"
                     /locus_tag="ISB17_003052"
     CDS             complement(14230..14925)
                     /gene="tsaB"
                     /locus_tag="ISB17_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP1980951.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(14983..16893)
                     /locus_tag="ISB17_003053"
     CDS             complement(14983..16893)
                     /locus_tag="ISB17_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP1980952.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            17025..17369
                     /locus_tag="ISB17_003054"
     CDS             17025..17369
                     /locus_tag="ISB17_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP1980953.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4320 a         4428 c         4204 g         4498 t
ORIGIN      
        1 atctatttag ttgaaaccat tgtaattatc cataaaccaa tcaacggcag tacactgatc
       61 cagacaaagt cgaatgatgt ttctattttc tatattttcc gccaactcag tttcatttgt
      121 ttaacctcgc tggcgctctt ttgttatggg aaagacaaca tccttgacaa caataagaaa
      181 aaaactggca ttctgttgct ggcgctgatt ccttttttag tttttcccct tctggcacac
      241 aatctgagca gttataacgc tgactattct ttgtatgtcg tcgattactg cccggacaac
      301 catactgcga cctggggaat caactataca aaaatattgg tttgtttgtg ggcattttta
      361 ctgttcttta ttatcatgcg tacacgatta gccagcgaac tatggccgtt aatagcatta
      421 ttatgtctgg catcgctatg ctgcaactta cttctactga ctctggatga gtataattac
      481 accatctggt atatcagtcg cgggattgaa gtttccagta aactgtttgt tgtgtctttt
      541 ctgatttata acatttttca ggaactgcaa ctctccagca aactggcagt tcatgatgtg
      601 ctgaccaata tttataatcg gcgctacttt ttcaacagcg tagagtcatt attgtcgcga
      661 cctgttgtta aggacttttg tgtcatgctg gttgatatta atcagttcaa acgcatcaat
      721 gcccaatggg gacatcgtgt gggtgataaa gtgctggtat caattgtcga tattatccag
      781 caaagcatcc gccccgatga tattttagcg cgactggagg gtgaggtgtt tggcttgcta
      841 tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg aacgtatgcg taaaaatgtc
      901 gaactcctga ccggctttag taacagatat gatgttcctg aacaaatgac catcagtatt
      961 ggcacggttt tttcaacggg tgacacgcat aatatctcgc ttgtcatgac ggaagcagat
     1021 aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag tgattattca tcatatttaa
     1081 gtgcaaaaat attcacagcc atgctttttg cgtggctttt gcatacaatt tattactatt
     1141 acccctaaat ttcccctccc cagcggcgca gagatgagta taattagcgc ccctgtgcca
     1201 ggccgcaatc gaactttatc tggttttctc gtttcactaa ccgaaggagt gccatttatc
     1261 atgaaattgc accatagaat gctccggcat tttatcgccg caagtgtcat tgtgctgaca
     1321 tcttccttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
     1381 tatatcgtgc agagagcaga ctcttccttt ctttatgata agtatcagaa tcagagtatt
     1441 gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtaccgcc agaacaacgg
     1501 cgcgctatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgct
     1561 cataaatacc agggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
     1621 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
     1681 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
     1741 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
     1801 tggtccttcc tgcaatcagg aagtattggt atcgatcgca aagatattga aaaagtattt
     1861 accgggcgta ccgtattatc gagcatttac caggatcagc gtactaaaca gaacgtgatg
     1921 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
     1981 aacaaaaaca atctgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
     2041 ctcaatgtca cgctaaccga tacagattcg gggcgcgaca ttatcatcaa ccagagcgaa
     2101 gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
     2161 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
     2221 ttgacggcgt taattttgct gaatatggtg cggatgcact tccgcttata ccaaaatgtg
     2281 tcgcgagaaa atattagtga tgcgatgact ggactctata atcgcaaaat tttaacccct
     2341 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgct
     2401 attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga gggggattta
     2461 gcgattacgt tattagctca ggcgattaaa cagtcgattc gtaaaagtga ttatgccatc
     2521 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
     2581 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agagatcggc
     2641 ttctcttccg gtatttacgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
     2701 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
     2761 gtggttgttt gcttgtaatc tcaggagcat gaaatgactg aaatggctaa aggaagcgtg
     2821 acgcatcagc ggttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
     2881 catgaagcgg taggtaaatg tgaagcagtg tcagaaattc gtggcaccgc tttaggccag
     2941 ggggctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggcg
     3001 atcctcgctg ccgatcaaca ggccgatctg agccaactcg ccagccatat aggcggatta
     3061 cgcgcttcgt tggccagccc ggcggaagtc gatgaactca caggctgtgt cttcggcgcg
     3121 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccccct actcttcgaa
     3181 cgatttgatg aaatcgcctt caatgcaggc atgctggata aatccgttat tttgaaaacc
     3241 gcggattatt tgcgtattgc gcaaccagaa ctggtcaatt tccgccgcac tgcgtaacta
     3301 gcctgtccgt tcaataagca gaacggacaa aaccagcaca gcagcaatgg caaaaaacga
     3361 tgacgtgata atcagtgtct cgacgaacat ttgatcgttc atagtatgcc cccgggagat
     3421 agctgttttc acgttattgt taacgtacac aaatggcagt ttgatgacag ttcgtctgat
     3481 tttttatgca aaaaacgtaa agattttcta cttcctgcct gcagcaagcg taaagtaagc
     3541 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
     3601 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
     3661 gttgattatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
     3721 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg ccagcgggac
     3781 gctaccgccc tcaaccctga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     3841 agtcggcgtt attgtctcct ggctgggggg gcgcggcgtg acgttaatgg gcagccagcc
     3901 gcaactggtc gccgggctgc tggtgggcac tgttttaggc gtggcgctgt ttcgcggcgt
     3961 accggttggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
     4021 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     4081 gccgttgttg aatcataccc cagtgtatga gcaactttct gtacagtatc gcctttcact
     4141 aatccctgaa gcgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     4201 gtctctttca ctatcagccg ggcaagattg cgttcactca tcgcaaaaaa gccagcccat
     4261 tgcctcaatg agccccactc gacgggctct ttcgccatca tctccaccat cgtgcggatt
     4321 ttaggatgag aagaaccggg caagtgcagc tgttgttgtg gttgctgagg gagttcatca
     4381 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     4441 gccagggtta aaattaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     4501 ggcattgtca ctgcgctggg ctcgataaac agaaaacaga gttcggcgtt agctgtcact
     4561 tgattactat gtttcacccc gcccggtatc caaaccgcat attgtggcgg caccatccac
     4621 aaagcatttt ctaccgtaca ggtaattgca ccatgtagtg ccagtatcaa ctgccctttg
     4681 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
     4741 gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg catcattttg
     4801 tccgaattta gcgatatttt gtcattttag cttgattcaa cataacaata aaaacggtaa
     4861 ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca ttaagcggta
     4921 aaaacaggat tttccttatc gctggcattc tgatgattgc cacaacatta cgcgtcacct
     4981 ttaccggcgc agcaccgtta ctggatacga ttcgttccgc ttactcgcta acgacggcgc
     5041 aaaccggctt attgaccacc ctgccattat tagcctttgc gctgatctca ccgctggccg
     5101 ccccggtggc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta cttttgatct
     5161 gtgcaggtat cgcaattcgc tctctccctt cgccttactt attatttggc ggtacagcgg
     5221 tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt aaacgcgatt
     5281 tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga gctgcggcgg
     5341 cactgggatc ggcgatggta gtgccgctgg ctttgaacgg ttttggctgg caaggcgcgt
     5401 tgctcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca cagtggcgaa
     5461 gtcaacaaca tgcaaatttg agtacctcac gcgccttaca tactcggggt atctggcgtt
     5521 cgccgcttgc ctggcaggta acattgtttc ttgggatcaa ctcactggtc tattacgtga
     5581 ttattggctg gcttccggcg atcctcatca gtcaaggcta tagcgaagca caggcgggtt
     5641 cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc ccacttttct
     5701 tacatcatgt gaaagatcag cgtggtattg cagcgttcgt tgccttgatg tgcgcagtgg
     5761 gcgcggttgg gctctgcttt atgccagcac acgcgatcac ctggactctg cttttcggtt
     5821 ttggttccgg cgcaacaatg atactggggt tgacgttcat tggtcttcgg gcaagttctg
     5881 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     5941 gtgggccgcc gctgatgggt aaaatacacg atgctaacgg tgactggtcc gtaccactga
     6001 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     6061 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcatgcgtg aaagggagaa
     6121 caaatacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     6181 atcgcgtact ggtcgaccgc ctctggccac gcggtatcaa aaaaaccgat ttagcccttg
     6241 atgagtggaa taaagaaatc acgccgtcaa cggagctgcg caaagccttt cacggcgaag
     6301 tcgtcgattt tgcaaccttt cgcgagcaat atcttgcaga actggcgcaa cacgagcaag
     6361 aaggaaagcg gctggcagac atcgccaaaa aacagccgct gaccctgctc tactcagcaa
     6421 aaaacaccac gcagaaccat gcgctggtgc tggcagactg gctacgtagc ttgtgatttt
     6481 agtacagcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     6541 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     6601 acaggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     6661 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     6721 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaggct
     6781 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     6841 tattacgcct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     6901 tcagcattta cccgccgtat cctggagttg tttcgtgtca gatcagatta tcgcccgcgt
     6961 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gttcgacagc ttctggagat
     7021 gctggaaatg gtcactgata tggagtcaac ctacctgacc aaagtggatg tcgaagcgcg
     7081 cctgcagcat ataatgtttg cccgtaatag ccagaaaatg cacatcccgg agaattttac
     7141 cgtctcgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgtt ttttcagcga
     7201 tgaagtcccc gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     7261 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     7321 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtc ttacagttat tcgccggact
     7381 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcgcgaag ctaatgctgc
     7441 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     7501 tgaaaatctg acgacgctgt tttctctcgc ccggcatctt aaccataaga taatgatcgc
     7561 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     7621 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     7681 tggtcgtctc ggcggtgatg agtttttggt tgtttcactg aacaacgaga atgcggatat
     7741 ttcgtcgctg cgagaacgta ttcaacaaca aatacgcgga gaatatcact taggtgatgt
     7801 tgatttgtat tatcctggtg ccagtcttgg catagtagaa gtcgatcctg aaacgaccga
     7861 tgcagacagt gccctgcatg ctgccgatat cgcgatgtat caggagaaaa aacataaaca
     7921 gaaaacacct tttgtcacgc attcagcgct acattcctga ggcgtattca catccttttg
     7981 attggtgata acatgcgaat cggtattatt tttccggttg taatctttat tacagcggtc
     8041 gtatttttag catggttttt tattggcggc tatgctgccc cgggagcata aagatgaaaa
     8101 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     8161 ggtggtaacg tcacatctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     8221 tacgcgttaa cttttaatct tcctgtagat aaatagcaca acaatcgcac caataacggc
     8281 aaccacgaag ctgccaaaat tgaagccatc gactttacca aagccaaaca gcgtgctgat
     8341 ccatccgccg actacggcac cgactatccc cagcaggata gtcataaaga atccaccacc
     8401 atctttacct ggcatgatcc acttcgccag aataccggca ataagcccaa aaataatcca
     8461 tgacagaatg cccattgttt cctcacttat ctgttttgca ttagcgggtt agtcgctgat
     8521 aaaaagcata gcacaacatc gggaggggaa gatttgtgac gagcatcacg gaggaatttt
     8581 ttgcgatggc gcagaaattg cgccatcaac gatcagtgat aattaccaac cacaaacatc
     8641 atgttcgttt tccgtgtcat aagaacgcac ggtattcacc agatctttta tcacttcagc
     8701 cgccacttct ggcaccagca aagtcatcgg cgtctctgtt tcataatcga cagaaacgcc
     8761 attgctgtta ttggtgacgg tcacggtata cgttgctttg cccatgattc atttcccgtt
     8821 atgaatgact ttccgttgtt gcgcgccttc catcaggact tcaggagcaa cgaagaagtc
     8881 aatgttgaaa taagtatcgt cagtcatggc ttcaatgttg tgccactttt ctggagggaa
     8941 caccgcaaac tgccccgctt cgataaggat cacctgatca ggctctgcac tgtgttcatc
     9001 agcgtagccg agatatttga ccgccccatg cataacggaa aggcgtgggt aaacccccgg
     9061 gcgcgttcct ttatcaagat gacgttcgaa tattccggca ggcgcagttt gtttattcca
     9121 gaaaggcgtt gagcgcgtat gaatataatt ctgtgggatt tgcagcatcc ttttccctcc
     9181 ttcggtgaat gcgctggaaa cggtttattc cagccgtttc agggtacgcc tgataatttg
     9241 cattttaaat accatttatt ggttactttt tagcaccgta tcagagaaga atcagggagg
     9301 attacaggtg ggaagcccgc ggcaacgcgg gctgaaagca tcaggattgc agcgtcgcca
     9361 gtcgggcggc gaaacccacg aacatcaaac caatcagtga gttgcccact ttagccagtt
     9421 tctttttggt acgtatgtac tgcgtgacaa aagccccaga aataatcagg aagctcaaat
     9481 agcagaaact caccagttcc agcgtcgtcg ccagaataaa gaatgaaatt cccgtatgtg
     9541 gggcattaac atcgataaac tgtacgaaaa acgacacata gaacaaaatg gctttcggat
     9601 tagtcaggct caaaattaac gcgcgtttaa aaatggcacc gtattggggc tcatcggatt
     9661 tggtctcgct atttttacct ttcagggtcg cgtagagaat tttactcccc agatagagca
     9721 aataaaacgc accaagataa cgtacgatgt tgaataatat cggggtggtc ttaattaatg
     9781 tcgccactcc agcccatgcc agaaacatca ataccgcatc gccaataaat acaccacagg
     9841 ccgcaagata accgcctttc ataccgctac tgacgctatt tttgagtaca aacagggtat
     9901 ttggccctgg caccaacaca ataaaaatgg ccccaaccag ataggtccag taattcagaa
     9961 ccccgtattc agcgaacaca ttaacctctt taattatctt tcgatcatgc gcgattaaag
    10021 gtgaatatgc taaccaatct gtagcggctt agaaaggaga aaatcaggtt ttaactcgat
    10081 atcaacccga taattgaatc attaatcagg catgctccag tgaaaaattc gggtagtgct
    10141 cggcaaaata ctggcgtaaa aattctaccg ttatccgcac tttcgctgac gtcgacagct
    10201 ttgaaacata aacggcccag acattcgctg gctggtaata ttccggtagc acttgtacta
    10261 aatgaccact ggcgggaaac agcctggcga catcgatttt tacgtggtcc ggaaaaacac
    10321 cgaaggcgaa tattcctcgc caggcggtag agtgaatgaa ggaacagaga atgaagtcgt
    10381 cattcaggaa tcggtattta ctcgtcgtgg tgtcgagcgc atttggcgtt aagctcaaat
    10441 aagttcattt gaaccgcctg ctgttggatt tgtagtttgc cgaagccgga ctgagcgcgc
    10501 ccagtccggc ttcggaagat tttactgtgg atattcctgc aataaattgt gtaatgcttc
    10561 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
    10621 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
    10681 gatcttgtgc gtttcgctga cgtcagtaat cagcgactta atcgctttaa acgctttacg
    10741 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgc
    10801 ctgcgctttt tccaccagcg ccagcaacgt tttaccgtgg aagcggattt cgctaccgga
    10861 taagcccagg ctgtccagtt cgcccagact tcccggcata taacgcgcta ccgaccacaa
    10921 atgctcttca cgcacgacaa agttcaccgc cagatcgcgc tctcgcgcct tgcgcaatcg
    10981 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaactgcc aggcattggt
    11041 gatatcacgc caggcgtctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
    11101 gcattcatcc agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagctttgc
    11161 ggtgatcggt aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag
    11221 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
    11281 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
    11341 cgtgtcaatc aagggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
    11401 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
    11461 ccagtcggtg atcccgagtg gatcgattag cgccagatgc tcaccatcaa aaagttgaat
    11521 taaacccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcca gggctatcgc
    11581 cggaaaggca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
    11641 gtaattcaaa ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
    11701 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
    11761 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
    11821 cttcggtact ttgtagcccg tgagctgacg gcggcaaaag gtcaccagtg actcttcggt
    11881 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
    11941 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
    12001 gttgggatag acgttaaaac cggaaaccag aatcatatct tttttacgat cgacaatgcg
    12061 caggaatcct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
    12121 gatttcatcg gtagcatccg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
    12181 acaaagctca cctggttgac caggtggtac ttcattatca tcatcatcca ccagtttggc
    12241 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catacgggtt
    12301 aacgctgacc agcggcgcgc actcggtcag gccataacct tccagcagat actgtccagt
    12361 cagtttaacc caacgttctg caaccacttg ctgaacaggc atcccaccgc ctgcggaaag
    12421 atgcagactg gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa
    12481 ggtgttaacg ccagtgatag cggtaaacgg atatttcgct aactctttta ccagccctgg
    12541 aatatcgcgc gggttagtga taagcaggtt ctgccctccc agttcgataa acagcaggca
    12601 gttaatggtc agtgcaaaaa tgtgatacag cggcagcgcc gtcaccacca gctctttgcc
    12661 cggatgcaac agcggaccat aggtcgcgtt aacctgttcc aggttcgcca gcatattgcg
    12721 gtgagtcagc atcgcgcctt tcgccacacc agtggtgccg ccggtgtatt gcaggaaagc
    12781 taaatcttcc ggcaccaatt ctggtttgac gtactgcatc cggtagccgt tgtgcagggc
    12841 gctacgaaat gaaatggcat ctggcagatg gtatttcggc accagacgct tgatgtattt
    12901 aacaacgaaa ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag
    12961 aataacgtgc tgaacggcgg ttttatcaac cactttttcc agagtgtggg caaagttaga
    13021 cacgataaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    13081 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    13141 cggatattgc aataagttag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    13201 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagtttgc ggaaggtcat
    13261 cacctccccc atattcacaa acgcgggttg atcggcatag cgtgcgaccg actgctcaaa
    13321 catatctacc agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata
    13381 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    13441 aaccccaagt gataaacatg ctattaacat aatattaact catcttacca gcttgataat
    13501 tacccaagga aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg
    13561 cagaaacagc ggaccggccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac
    13621 aactgtttgt actctcgcgg ggccgggatt ataccagccc catccgccgt agccataggg
    13681 ccagccacga ccgccataaa accatggttc aatcggctga ggcggcataa tcacctgctg
    13741 ggttaaatgc caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt
    13801 accgattttg ccgtcaaccg caccggtgat tggcccgact accgtcacca gttgtccgcg
    13861 gaaatccacc gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc
    13921 tcccagcgtc ggtctggctc cgctgtccag tggtacggtg gcaatttcca ggcgggtttt
    13981 cccttgctgg ttttgtacag caaccacttt gccgccaaag cgagcctcct gaccaacgta
    14041 cagctgcggc gcactcatca cccgaactaa atcttgttgc ggcgtgggac tactgccttt
    14101 aatggcgtcc ggcacagtaa cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    14161 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    14221 tcttaagatt cattctttgc ccggaagttt cttccatgcg acattgttac gtaaataaac
    14281 tggttcggca tgttccaccg ccaccgtttt accctcggca aacatctggc acgcaatcgg
    14341 cagcatatct tctgcagcgg gcagtaacac ttcgccatcg tgcaaaacca gcccgctctc
    14401 tttaccgaga tccggccagg cttgccagcc cgtccctacc gtcacccatt cgccggaaag
    14461 ctgttgcatt cgttcatgaa ccagttcggg tttgagtacg gcttcggttt cttcaccgtg
    14521 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    14581 aatagctgcc agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccatcgtcat
    14641 tagcgtggag acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatacc
    14701 aataccaatg cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgtttat
    14761 gtcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    14821 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc
    14881 gacagagcag gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    14941 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    15001 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgc ggcgctggcg gcagactggc
    15061 gagaaacgtc gcgccgtaag ggcgcatcac cagccgattg tcgcaaatca ccagcacgcc
    15121 gcgatcgtcg gcgtcgcgaa tcaatcgccc taccccctgc ttgagagtaa tgaccgcatc
    15181 cggcagttgt acttcatcga acggatcacc accgcgcaaa cggcaatctt ccatgcgtgc
    15241 ttttaatagt ggatcatccg gcgaggtaaa cggcaatttg tcgataatca ccaatgacaa
    15301 tgtatcgcca cgcacgtcca ccccttccca gaaactgctg gttgccacaa gaagcgcgtt
    15361 accggcgctg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac
    15421 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg cgtgcgaggt
    15481 acaaagcata aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag
    15541 ttgccgtgcg gaacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    15601 ctggcggctg taatcaaagg ggctgggtaa cagcaatgac tctgcctgtt caatgccaag
    15661 ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc gttgctgagg taaagatcca
    15721 gctaccggtt ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    15781 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    15841 ctcttttagc cgtttcagcc gtgtgcgata taacgtggcg cgctcaaatg ccgcatccag
    15901 caaggcggaa cgccccagcg acagtttcgc cacgtcataa caaagttcca gagtgtcatc
    15961 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    16021 ataaccaggc tcaccgagtt gcaggcgaaa atcctgcgca ctctgggcaa gacgatccgc
    16081 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    16141 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    16201 gggcagctgg tgggcttcgt cgaagatcat gacgtccgcc tccgggatca gttcgccaaa
    16261 accgctctct ttcaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    16321 ggcgtccatc gcttttttgc gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    16381 gctgccgagg cagttatcgt tggtgctggt gaccagcggc cacgcctgtg aatcttccgc
    16441 cacgctgacg caggtgctga tatcaccatc gattgtttga ttagaccagg agcgcagcag
    16501 gatcacatcg cttaagattt gtaccggcag atcgcccccc gccaaagcct gctgttcgag
    16561 acgttcgagg cagaggtagt ttgagcgccc tttcagcagc gccacattgc ccgtgtattt
    16621 caatgccttt gagaccgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga
    16681 gccggtcgag attatgactt tctttttcgc ccgcagcgca ggggccaggt aagcgtaggt
    16741 tttgcccgta ccggttccgg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    16801 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    16861 cagctgaccg tcttgtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    16921 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcgacct cacattgtgg
    16981 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
    17041 tcgatgccga agcccgctgg tctgatgtgg taatccacaa caacacgctc tactacactg
    17101 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacg ctggcgcaga
    17161 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    17221 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    17281 cgggtcatgc gccggtgcgc tgcacggtac aagcaggttt gatgaacccg aagtataaag
    17341 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    17401 atttatcgac aatactggtc ggttttgcaa aaatcgacca gagattaaga
//
LOCUS       DACADA010000093        17419 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.095, whole genome shotgun sequence.
ACCESSION   DACADA010000093 DACADA010000000
VERSION     DACADA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.095"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            71..574
                     /locus_tag="ISB17_003055"
     CDS             71..574
                     /locus_tag="ISB17_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAP1980954.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(641..2098)
                     /gene="nuoN"
                     /locus_tag="ISB17_003056"
     CDS             complement(641..2098)
                     /gene="nuoN"
                     /locus_tag="ISB17_003056"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAP1980955.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2105..3634)
                     /gene="nuoM"
                     /locus_tag="ISB17_003057"
     CDS             complement(2105..3634)
                     /gene="nuoM"
                     /locus_tag="ISB17_003057"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAP1980956.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3798..5639)
                     /gene="nuoL"
                     /locus_tag="ISB17_003058"
     CDS             complement(3798..5639)
                     /gene="nuoL"
                     /locus_tag="ISB17_003058"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAP1980957.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5636..5938)
                     /gene="nuoK"
                     /locus_tag="ISB17_003059"
     CDS             complement(5636..5938)
                     /gene="nuoK"
                     /locus_tag="ISB17_003059"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAP1980958.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5935..6489)
                     /gene="nuoJ"
                     /locus_tag="ISB17_003060"
     CDS             complement(5935..6489)
                     /gene="nuoJ"
                     /locus_tag="ISB17_003060"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAP1980959.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6501..7043)
                     /gene="nuoI"
                     /locus_tag="ISB17_003061"
     CDS             complement(6501..7043)
                     /gene="nuoI"
                     /locus_tag="ISB17_003061"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAP1980960.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7058..8035)
                     /gene="nuoH"
                     /locus_tag="ISB17_003062"
     CDS             complement(7058..8035)
                     /gene="nuoH"
                     /locus_tag="ISB17_003062"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAP1980961.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8032..10758)
                     /gene="nuoG"
                     /locus_tag="ISB17_003063"
     CDS             complement(8032..10758)
                     /gene="nuoG"
                     /locus_tag="ISB17_003063"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAP1980962.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10811..12148)
                     /gene="nuoF"
                     /locus_tag="ISB17_003064"
     CDS             complement(10811..12148)
                     /gene="nuoF"
                     /locus_tag="ISB17_003064"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAP1980963.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12145..12645)
                     /gene="nuoE"
                     /locus_tag="ISB17_003065"
     CDS             complement(12145..12645)
                     /gene="nuoE"
                     /locus_tag="ISB17_003065"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAP1980964.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12648..14450)
                     /gene="nuoC"
                     /locus_tag="ISB17_003066"
     CDS             complement(12648..14450)
                     /gene="nuoC"
                     /locus_tag="ISB17_003066"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAP1980965.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14544..15206)
                     /locus_tag="ISB17_003067"
     CDS             complement(14544..15206)
                     /locus_tag="ISB17_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAP1980966.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15222..15665)
                     /gene="nuoA"
                     /locus_tag="ISB17_003068"
     CDS             complement(15222..15665)
                     /gene="nuoA"
                     /locus_tag="ISB17_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAP1980967.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(16295..17233)
                     /gene="lrhA"
                     /locus_tag="ISB17_003069"
     CDS             complement(16295..17233)
                     /gene="lrhA"
                     /locus_tag="ISB17_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAP1980968.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4341 a         4878 c         4533 g         3667 t
ORIGIN      
        1 aacatgcgca ttttattttc tttcgtctgt tttaagatgc gaaacatgaa attcaacgga
       61 ggaaaaataa atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca
      121 actgcttgag tctactaaaa aacctttgat ggataatata catgacacgc tttcaggatt
      181 acgccgactg gatattgata aacgctggga ttttttacac tttggcctga cgggtacatc
      241 tgcatttgac cccgcgaaga atgacccgct aagtcgcgca gtgcttggtg aacacagcct
      301 agaagatggc atagatggct tcctgggatt gacctggaat caggagttag ccgcgacaat
      361 cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact
      421 caatgaaatg gagatttatc cgggagtaac cttttcagaa gagttagagg ggcaactctt
      481 tgcttcgatc atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      541 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcaccc aaaaaaaccg
      601 ccgaacatgt cggcggcttt ctgacttaca aactgactgc ttacatcagc ggcattgcta
      661 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      721 agatcagcac cacgataccg cctgcgctgt actgccagtt tgatggtgca tcgcgacccg
      781 gttgttccgg agcgtgaagg tataagctca ccgccacacg caggtagtag tagaggccga
      841 ttgccgaacc gacaactacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg
      901 ccagcacgta gaacttaccg ataaagccca gcgtcattgg gataccagcc agtgaaagca
      961 tcatcaccgt catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga
     1021 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1081 cgccgaggct gctgaacaga taacctgcca ggtaaacccc taccgcttcc atcgacatct
     1141 cgccggtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1201 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1261 aggcaaaggc gataatcgcc agcactacgc gaatcgcttc actgtcaccc accggtgcgt
     1321 aaaggaacaa acgcatcacc acgccgaaga tagcgatttt gctcgccgtc gccaggaaag
     1381 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1441 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1501 cgttgagcat accgtcgccg aggtttttac ccagtgcgac aaacgacagg tcgccagact
     1561 gcgcatacac cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1621 tgtatttgat actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1681 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1741 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt
     1801 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1861 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagtggcgta acgtccatag
     1921 cgcccgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1981 gcgtagcgtt gagaaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     2041 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2101 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2161 cggagtgcga ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca
     2221 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2281 tctggctttt cgctttaccg aaataagcgc gatgcagcat cgccagcgaa taaacagatg
     2341 caaagaccag cccaaaggta gagataacgg taattaccgg gacaacctgg aagctaccga
     2401 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2461 caaagaacag cgacagtgct ggcagccact tcatcttgct ccacagaccg cccatcatgc
     2521 gcatgtcgcg ggtatggata cgttcataga gctgaccgca gagaataaac agacccgccg
     2581 ctgacaagcc gtgtgcaatc atctggatta ccgcgccctg gtaggccaac tggctaccgg
     2641 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2701 tgatatcggt ctgggcgaag gccatccacg caccatagaa gatgccgata acacccagcc
     2761 acatagcgat tggcgcgaac tctgccgacg cgttcgggaa cagcggtagg gagaaacgca
     2821 gcagaccgta agcggcggtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2881 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttcactg
     2941 cgaaggcgat gaagaagccc agcatcaaca ggtattccac gccgttagac atcggcgtat
     3001 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acgaaaacca
     3061 gcgcctggat ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3121 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3181 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3241 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctggt
     3301 atttttcgat ctctttccac gaacagagta ccgccagcac accgagcaga ccggtcagca
     3361 cgaccatcag caacgacaga ccgtcaatgg cgagatggat agagatacca aaacgcggga
     3421 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3481 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gttaccagcg
     3541 cgatccagcg cggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccga
     3601 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3661 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3721 agtgtcggat gcgccgcgaa gacggcttat ccgacctacg ttcttatatg cccaggggca
     3781 atcccacaat ccttaactca acgcagtacc atcaacagag ccagcaccac gaccgcaccg
     3841 atgctcatgg atgccacata ccagcgcaga taaccgttct cgcttaacag cagaccttta
     3901 cctgcaaagc gggaaaggac agccgggatg ttcatcattg agttcagcgg atcgcgtttc
     3961 agcaaccagg caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag
     4021 ccccaggcgt tgtaccacca ggtacccagc agacggcccg gcgcactgtt ggcgatggag
     4081 gtcaccagag tacgtttacc cagccacagc caggcagcca gcagaatgcc gaccaccgcg
     4141 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagctc cgtcgtttgc
     4201 ggaagcacgc cctgcagcgg cggtacaatc agtgcaccaa cgaaggtgga aaggatcagc
     4261 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4321 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4381 accagacctg ccaccatcag attgatatgt ccattcgcca tcgcacccgc gaggatctca
     4441 tccttactga agaagcccgc agtgaccagc ggcagtgccg atagtgctgc gccgcccacc
     4501 aggaagcaga gataaaccag cggaatagat ttacgcagac cacccatctt aaagatgttc
     4561 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4621 aacgcgtggg tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4681 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4741 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc
     4801 agaacttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4861 gttaccattg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4921 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4981 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     5041 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagcgc aaaagcgagg
     5101 aagacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5161 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5221 tacatcaaca gtaggttatc ggcgagaacc agaactacca tgctggcgat gaacaggttg
     5281 gtgtaagcga agaagcgaga gtagccctct tctccgcgca tataccagga ggcatacatg
     5341 tgaataagga aacccacacc agtgaccact gagagcatgg tcagcgacag gccgtccagc
     5401 accagattga aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggcta
     5461 tatgcctgct caccgttagc gaagaagtca acgccgataa aggcggttac cagcgccgcc
     5521 aggcccacag agccaacgcc gacgatcgcc gagacgtttt cagaccagcg cccacgggag
     5581 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5641 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5701 gcagcgcaag gccgatactc gcttccgccg ccgcgaggct gatggcgaga atgtacatca
     5761 cctgaccgtc ggtctggccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt
     5821 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5881 ttaagccaag aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5941 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     6001 acgctcttca cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc
     6061 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6121 tggcgtaccg tcgatacctt gatcgttaac accgagaatg gcgtaaacaa tcaccaccag
     6181 catgatggct gacaaaattg ccggaccaat ccacacctgc ggtttcaacc actggcgttc
     6241 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6301 aatggcaccc gcatagacga taatttccag cgcaccggca aagtaagcgc ccagtgagaa
     6361 gaacacccca gagatcgcca gcagcgaaat aatcaggtac agcagcgcgt gtaccggatt
     6421 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6481 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6541 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6601 tattccgggt atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc
     6661 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6721 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6781 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6841 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccggatcgcg ggtcagaacg
     6901 atacgaccac gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg
     6961 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     7021 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7081 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7141 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7201 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7261 caccgaagaa cagcgtcacc atcagtgcag agatggtcac aatcccgatg tattcgccca
     7321 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7381 gctcggcttc cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa
     7441 aggtaataaa accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga
     7501 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcaaggaa agcccgagga
     7561 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcagc gagtatttgt
     7621 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7681 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaattg
     7741 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7801 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7861 ggagcgaacc gccccagcca acacggttag gtccataacg gttctggaac agacccagca
     7921 ggcgacgttc gccaaagctc atgaatgccc cacaggtgac taccaccagc aggatcacca
     7981 ccgctttaag gacggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     8041 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc
     8101 catcggtaag cccacctgcc ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac
     8161 cgtgttgcca tcgtaactaa aggagacgcg tgtacctgcg ttcaccccca acttcgcggc
     8221 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8281 ctgtgacaat tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc
     8341 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccgtttt cgctggtttc
     8401 aaacagacgc acgcccggat cgccaaaacg cagtttgccg cccacttcgt cctggaattt
     8461 gttccacgcc tgcggggagt tccagcctgg tgcccaggca aacggcactt gcgaacggtg
     8521 cgcggtcggc tggttgttac cttccatcga gaaggtgaac atagtgtcaa tatcctgcgg
     8581 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tatagcgatg
     8641 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8701 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacgtggt cgagctgcgt
     8761 ccagtccact tcacggctca gcagggtgct atgcagcgag tgcagccagc gccagctttc
     8821 cagcatgaca gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc
     8881 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aagctggcgg cggaaagtac
     8941 cagatgggcg ttttccataa tcgctgtgcg ttgatggtca accaccatca ccagcggcgc
     9001 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9061 caccaccacc gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgatcc
     9121 accgcccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccgac
     9181 gtcagcgccg cgacctttca gggctttcgc cacgttagcc gcagcctgaa tcacttcagc
     9241 gctaccagcg ttcgtcccgg agataatcaa cggtttcttc gcaccagcca gtgcctgcac
     9301 gatgacgtcg attttgcttt gcagctcagg ctcaataccg tcaaccgctg gtgcagagtt
     9361 atccagcgca tgagcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9421 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9481 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9541 tgccgccatt tcgcgcgctt tacctttcac cgcctgacgc actgccagcg cgacgcgcgc
     9601 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9661 cagagccgga gtataaatgc cgccttcacg cagtactttc agcgccagtt gcagacgttc
     9721 ctgctcaccg tgagcgatac cggtatagaa gttttcttcg cccaccagct cacgcagcgc
     9781 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9841 cagaatatct gctgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9901 acgacgctgt actggctgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg
     9961 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
    10021 cagttcgcca taacgctcgc cggggctgat gttacagccg atggaacatt gctggcagat
    10081 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10141 gaatacgccg gtcggacaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc
    10201 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca ggtccgtacc
    10261 gtcagcgtaa tctttgtagt aacgcacgca gcggtagcag gcgatgcagc ggttcatttc
    10321 gtgagagatg aacggcccca aatcctgatt acggtgagta cgtttggtga aacggtagcg
    10381 acggaagctg tgtccggtca tcacggtcat atcctgaagg tggcagttac cgccctcttc
    10441 acataccgga cagtcgtgcg ggtggttggt catcaaccac tcgaccacgc tttcacggaa
    10501 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10561 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10621 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10681 cagagacaga caagcttcca gcaggttgtc cgccccgttg acctcgtatt ctttgccgtc
    10741 tacatgaatt gtagccatta gcatgcttcc agttttctca gtcagagact gagcgttaat
    10801 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10861 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10921 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc ggacctaaga
    10981 atcgacacag ttgctcaagt gtttcgatat cgcccggctg accttcacca cgctccagcg
    11041 cacgcagaat tttcacgctc cacggcagac cgtcgcggca tggcgtacac cagccgcagg
    11101 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgtta atctcgtggt
    11161 caaccgccat cgccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt
    11221 ccatcggcag gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgccg cctggctgcc
    11281 aggctttgaa tttcaggccg tcacgcatac caccagcgta atcttcaagg atttcacgcg
    11341 cggtggtgcc gaacggcagt tcccacagac ccggattttt cacccgaccg gagaagccca
    11401 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11461 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt aacgcaggtt ggtttacccc
    11521 atgcaccgga ggttgccggg aagggcggct tcgagcgtgg gttagcgcga cgcccttcca
    11581 gggagttgat taacgctgtt tcttcaccgc agatgtagcg ccctgccccg gtatggacga
    11641 acagttcgaa atcgaaacct gttcccataa tgtttttgcc aagcagaccc gcttcggtgg
    11701 cttcggcaat ggcacggcgc agattcactg ccgcttcaat atattcgcca cgcaagaaga
    11761 tgtagccacg atacgctttc agcgcaaacg cagaaatgag cataccttcc accagcaggt
    11821 gcggtagttg ctccatcagc agacggtctt tataggtgcc cggctccatt tcatcggcat
    11881 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11941 cagtagaaaa gcccgcgccg ccgcgccctt tcagaccaga gtcttttacc tgattaacga
    12001 tttcgtccgg agacagcccg gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc
    12061 gatattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12121 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12181 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12241 ccccaggcag caagttggca gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt
    12301 gatgttcagt tttttctcca gcgccgcctg aataccctgg taaccgttga tatgacagac
    12361 cacgctgtcg caataacgga tcacatggcg gcctactggc tgacggaaga tctggctata
    12421 aaacgttgcc acgccttcga cgtcgctcgc cggaataccc agcacatcgg cgatcgcgtg
    12481 gatcgcgcca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12541 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12601 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12661 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12721 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtacggat acgggtgcga
    12781 taactcatgg tgctgccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12841 accatctgga aggattcatt ggcaggcatc accggacccc acgacacttg caggaagtgg
    12901 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcgga
    12961 tgatccgcct tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    13021 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca
    13081 cccaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13141 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13201 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtgtt ttgcagcgcc
    13261 gctttctcgt aagacgccag acgtttaggc atccagtcga ggaactcacg cagcaggcga
    13321 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13381 cgaaaaccag tgattgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13441 aacactggcg tcatcgcgcc gacgtcctga ataaaggtgg agatatacag caggtgactg
    13501 ttgatgcgga acagttcgga gagcattacg cgaatgacgt taacgcgatc cggcacggtg
    13561 atcccagcca gtttctctac cgccagcaca taaggcattt cgttaacgca gccgccgagg
    13621 tattcgatac ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc
    13681 tcagcgccac ggtggtggta accgatgtct ggtacgcagt cgacaatctc ttcgccatca
    13741 agctgcaaaa cgatacggaa agcaccgtgc gcagacgggt ggttcggacc gaggttgagg
    13801 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13861 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13921 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13981 cggcgcaggt tcgggtgacc gtcgaatgta atgccaaata gatcccaggt ttcacgctca
    14041 taccagttag cgttcgggaa tagtttggta aaggtcggta cgtgcaggtc gttttctgcc
    14101 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14161 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14221 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc cacttccagt
    14281 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14341 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcgcgg
    14401 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14461 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14521 ttccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14581 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14641 tcgccaacca cccaggagag cggacgacgt tctttaccga tagattcctg caacagcatc
    14701 agtgcctgca tgtacgcttc aggacgcggc ggacagcccg ggatatacac atcaaccggg
    14761 atgaatttat caacgccctg cacaacggaa taaatatcgt acataccgcc ggagttggca
    14821 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacaa acgctgaata
    14881 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14941 gaagcacgca atacttcagc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    15001 accatctcaa cgtaacagca ggaaaggccg aagttatacg gccaaattga gtttttacga
    15061 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15121 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15181 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15241 atactgtccg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15301 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15361 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15421 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15481 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gaacgttttt tgacctcgcg
    15541 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15601 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15661 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15721 cacatcgatt catggggagg aataaaaaaa accttacaat aactgcagaa actattttat
    15781 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaagg
    15841 tttcttcaca ctgatttaca caatgacaac aacatttgca tttggcgccc tttctcccca
    15901 gatcattgac gactccagga aaattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15961 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    16021 attttcttga tctgtgacac gcttttgtca ttctataaca aaaacgcagc aacaaattca
    16081 cgtatttttt aacattattg tagcaggaga tttttttcag gtgattattt gtgcgtacgg
    16141 ggcgtgaatc tctggtgatt gaaaaatgaa cagttttata cgttctgcac gatacgagga
    16201 aggcttttta ctaaaaaaaa gccgctgggg tttaaaataa acccagcggt tcgtttttac
    16261 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    16321 atgagtcatc tccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    16381 ccatcgtctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    16441 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg
    16501 gactcatcat ctcgaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    16561 cggccggaag cgtcgaggcg acataagcca gtcgccaggg aatatctgct ttattaagcg
    16621 tcgccagcac catatcgcga aacggacttg gatcatccag caacacaaga gggatcggct
    16681 cacctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt
    16741 tcaatgcctt aaaggtagaa ggacgatggg tggttaccat caaatccact tcctgggatt
    16801 ccagcatttc tgccatgtag gcattacgtt taacgcggac atccagcgct agtttcggat
    16861 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    16921 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    16981 taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    17041 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    17101 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    17161 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    17221 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    17281 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    17341 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    17401 atgcctgaag tagatcaca
//
LOCUS       DACADA010000094        17409 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.096, whole genome shotgun sequence.
ACCESSION   DACADA010000094 DACADA010000000
VERSION     DACADA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.096"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            23..1459
                     /gene="gspA"
                     /locus_tag="ISB17_003070"
     CDS             23..1459
                     /gene="gspA"
                     /locus_tag="ISB17_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HAP1980969.1"
                     /translation="MREKHQTWRLCYLLGEAGSGKTRLAQQLQKDKHRRVITLSLVVS
                     WQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIENAHLNHRR
                     ILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHELKASILEGQN
                     IDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTCVFTAKQWRMIYRVL
                     GDNRPRKMQLAVVMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPVVKQDMTKDIAH
                     VVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTLVDQNLPWIASL
                     KVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLWKMSPEGESTIT
                     RDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLKTDGVVGFSTLV
                     HLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1461..1880
                     /gene="gspB"
                     /locus_tag="ISB17_003071"
     CDS             1461..1880
                     /gene="gspB"
                     /locus_tag="ISB17_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HAP1980970.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2118..2429
                     /gene="rpsJ"
                     /locus_tag="ISB17_003072"
     CDS             2118..2429
                     /gene="rpsJ"
                     /locus_tag="ISB17_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP1980971.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2462..3091
                     /gene="rplC"
                     /locus_tag="ISB17_003073"
     CDS             2462..3091
                     /gene="rplC"
                     /locus_tag="ISB17_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP1980972.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3102..3707
                     /gene="rplD"
                     /locus_tag="ISB17_003074"
     CDS             3102..3707
                     /gene="rplD"
                     /locus_tag="ISB17_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP1980973.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3704..4006
                     /gene="rplW"
                     /locus_tag="ISB17_003075"
     CDS             3704..4006
                     /gene="rplW"
                     /locus_tag="ISB17_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP1980974.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4024..4845
                     /gene="rplB"
                     /locus_tag="ISB17_003076"
     CDS             4024..4845
                     /gene="rplB"
                     /locus_tag="ISB17_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP1980975.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4862..5140
                     /gene="rpsS"
                     /locus_tag="ISB17_003077"
     CDS             4862..5140
                     /gene="rpsS"
                     /locus_tag="ISB17_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP1980976.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5155..5487
                     /gene="rplV"
                     /locus_tag="ISB17_003078"
     CDS             5155..5487
                     /gene="rplV"
                     /locus_tag="ISB17_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP1980977.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5505..6206
                     /gene="rpsC"
                     /locus_tag="ISB17_003079"
     CDS             5505..6206
                     /gene="rpsC"
                     /locus_tag="ISB17_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP1980978.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6219..6629
                     /gene="rplP"
                     /locus_tag="ISB17_003080"
     CDS             6219..6629
                     /gene="rplP"
                     /locus_tag="ISB17_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP1980979.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6629..6820
                     /gene="rpmC"
                     /locus_tag="ISB17_003081"
     CDS             6629..6820
                     /gene="rpmC"
                     /locus_tag="ISB17_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP1980980.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6820..7074
                     /gene="rpsQ"
                     /locus_tag="ISB17_003082"
     CDS             6820..7074
                     /gene="rpsQ"
                     /locus_tag="ISB17_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP1980981.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7239..7610
                     /gene="rplN"
                     /locus_tag="ISB17_003083"
     CDS             7239..7610
                     /gene="rplN"
                     /locus_tag="ISB17_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP1980982.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7621..7935
                     /gene="rplX"
                     /locus_tag="ISB17_003084"
     CDS             7621..7935
                     /gene="rplX"
                     /locus_tag="ISB17_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP1980983.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7950..8489
                     /gene="rplE"
                     /locus_tag="ISB17_003085"
     CDS             7950..8489
                     /gene="rplE"
                     /locus_tag="ISB17_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP1980984.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8504..8809
                     /gene="rpsN"
                     /locus_tag="ISB17_003086"
     CDS             8504..8809
                     /gene="rpsN"
                     /locus_tag="ISB17_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP1980985.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8843..9235
                     /gene="rpsH"
                     /locus_tag="ISB17_003087"
     CDS             8843..9235
                     /gene="rpsH"
                     /locus_tag="ISB17_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP1980986.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9248..9781
                     /gene="rplF"
                     /locus_tag="ISB17_003088"
     CDS             9248..9781
                     /gene="rplF"
                     /locus_tag="ISB17_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP1980987.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9791..10144
                     /gene="rplR"
                     /locus_tag="ISB17_003089"
     CDS             9791..10144
                     /gene="rplR"
                     /locus_tag="ISB17_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP1980988.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10159..10662
                     /gene="rpsE"
                     /locus_tag="ISB17_003090"
     CDS             10159..10662
                     /gene="rpsE"
                     /locus_tag="ISB17_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP1980989.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10666..10845
                     /gene="rpmD"
                     /locus_tag="ISB17_003091"
     CDS             10666..10845
                     /gene="rpmD"
                     /locus_tag="ISB17_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP1980990.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10849..11283
                     /gene="rplO"
                     /locus_tag="ISB17_003092"
     CDS             10849..11283
                     /gene="rplO"
                     /locus_tag="ISB17_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP1980991.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11291..12622
                     /gene="secY"
                     /locus_tag="ISB17_003093"
     CDS             11291..12622
                     /gene="secY"
                     /locus_tag="ISB17_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP1980992.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12654..12770
                     /gene="rpmJ"
                     /locus_tag="ISB17_003094"
     CDS             12654..12770
                     /gene="rpmJ"
                     /locus_tag="ISB17_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP1980993.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12917..13273
                     /gene="rpsM"
                     /locus_tag="ISB17_003095"
     CDS             12917..13273
                     /gene="rpsM"
                     /locus_tag="ISB17_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP1980994.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13290..13679
                     /gene="rpsK"
                     /locus_tag="ISB17_003096"
     CDS             13290..13679
                     /gene="rpsK"
                     /locus_tag="ISB17_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP1980995.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13713..14333
                     /gene="rpsD"
                     /locus_tag="ISB17_003097"
     CDS             13713..14333
                     /gene="rpsD"
                     /locus_tag="ISB17_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP1980996.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14359..15348
                     /gene="rpoA"
                     /locus_tag="ISB17_003098"
     CDS             14359..15348
                     /gene="rpoA"
                     /locus_tag="ISB17_003098"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP1980997.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15389..15772
                     /gene="rplQ"
                     /locus_tag="ISB17_003099"
     CDS             15389..15772
                     /gene="rplQ"
                     /locus_tag="ISB17_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP1980998.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15879..16247
                     /locus_tag="ISB17_003100"
     CDS             15879..16247
                     /locus_tag="ISB17_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAP1980999.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16258..16683
                     /gene="zntR"
                     /locus_tag="ISB17_003101"
     CDS             16258..16683
                     /gene="zntR"
                     /locus_tag="ISB17_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAP1981000.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16739..16957
                     /locus_tag="ISB17_003102"
     CDS             16739..16957
                     /locus_tag="ISB17_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAP1981001.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16954..17367)
                     /gene="mscL"
                     /locus_tag="ISB17_003103"
     CDS             complement(16954..17367)
                     /gene="mscL"
                     /locus_tag="ISB17_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP1981002.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
BASE COUNT         4471 a         4045 c         4667 g         4226 t
ORIGIN      
        1 aagagaagtc attctttcct ggttgcgtga gaagcatcag acctggcgtc tgtgctattt
       61 gttgggtgag gctggaagtg gaaaaacccg gctggcgcag caactacaaa aagataaaca
      121 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      181 taccgacgat aatgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      241 ggcgggtcaa ttactgcatg cacttcatcg aactgatagt cgctgccctc ttattattat
      301 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      361 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      421 taaaaaacag ggcattgaac ttgtctcgac aggtcgtctg acggagcacg agcttaaggc
      481 aagcatcctt gaaggtcaga atattgacca gcccgatctc cttttaaccg ccagagttct
      541 gaaacggata gctttattat gtcggggaga tcgcagaaag ctggcgcttg ccggcgaaac
      601 aattagctta ttacagcaag ccgagcagac ctgcgttttc actgcaaaac aatggcgaat
      661 gatttaccgc gtattaggtg ataaccgccc ccggaaaatg cagcttgccg tggtgatgag
      721 tggaaccatt atcgcactta cctgtggttg gctactgctg ccctctttca cagctccgtt
      781 acccgttcct gcgtggctga tacccgtaac gccagtagtt aaacaagata tgactaagga
      841 tattgcccat gtggtgatgc gcgatagtga ggcgctaagc gtgttatacg gcgtatgggg
      901 gtacgaggtc ccagccgatt cggcctggtg cgatcaggca gtaagagcgg ggctggtatg
      961 taaaagtggg aatgcttctt tacaaacatt ggtagatcag aatttgccct ggatcgcatc
     1021 gcttaaagtc ggggacaaaa aattgcctgt tgtggtcgtt cgcgtcggtg atgctaccgt
     1081 tgatgtactc gtagggcagc aaacctggac gctgacacat aaatggtttg agttggtatg
     1141 gacgggtgat tatcttctgt tgtggaaaat gtcgccggaa ggagagagta ccattacgcg
     1201 tgatagcagc gaggaagaga ttctctggct ggaaacgatg ttaaaccgcg cattgcatat
     1261 ttcgacagaa tcttcggcag agtggcgtcc cttgttggta gaaaaaataa agcaatttca
     1321 aaaaagccat cacttgaaaa ctgacggtgt tgttggcttt tcaacgttag tgcatctctg
     1381 gcaggtggca ggggagagtg cctacttata tcgggatgaa gcaaatattt ccccggaaac
     1441 aacagtgaag gggaaatgac atgtttgaat tctatatcgc cgcgcgtgag cagaaagaaa
     1501 caggtcaccc tggtatcttc tcccgacaaa aacacagcac cataatatat gtcatttgtt
     1561 tattgctgat ttgcctgtgg tttgcaggaa tggtgttggt tggtgggtat gcccggcagc
     1621 tatgggcact ttggatagta aaagcagaag tcactgtaga tgctgaaacg cccgctttta
     1681 aacaatcaac acaacactat tttttcaaaa aacagccact ccccgtcgtc gaatccgtcg
     1741 aggaggaaga tgacccagga gtcgcggttg agaatgcccc ctcttcttct gaagacgaag
     1801 acaatactgt agaagaaagc gacgagaaag ccggtttaag agagcgtgtc aaaaatgcgc
     1861 tgaacgaact tgagagataa cccaaaggct gtttacttta ctagcaatac gcttgcgttc
     1921 ggtggttaag tatgtataat gcgcgggctt gtcgtagttg acagcaggtt caatctgaac
     1981 ctcagttagc cgaatttggc tacctaacaa tgctcccaat cggggagcta cgtaagaacg
     2041 gttacactct cccatcaatc gtaatgggtc tgaggagtaa tcattttcgt ttataaaata
     2101 attggagctc tggtctcatg cagaaccaaa gaatccgtat ccgcctgaaa gcgtttgatc
     2161 atcgtctgat cgatcaagca accgcggaaa tcgtcgagac tgctaagcgc actggtgcgc
     2221 aggtccgtgg tccgatcccg ctgccgacac gcaaagagcg cttcactgtt ctgatctccc
     2281 cgcacgtcaa caaagacgcg cgcgatcagt acgaaatccg tactcacttg cgtctggttg
     2341 acatcgttga gccaaccgag aaaaccgttg atgctctgat gcgtctggat ctggctgccg
     2401 gtgtagacgt gcagatcagc ctgggttaat caggtcattg agcgattgag aggttgaaac
     2461 aatgattggt ttagtcggta aaaaagtggg tatgacccgt atcttcacag aagacggcgt
     2521 ttctatccca gtaaccgtaa tcgaagttga agcaaaccgc gttactcagg ttaaagacct
     2581 ggctaacgat ggctaccgtg ctattcaggt gaccaccggt gctaaaaaag ctaaccgtgt
     2641 gaccaagcct gaagctggcc acttcgctaa agctggcgta gaagctggcc gtggtctgtg
     2701 ggaattccgc ctggctgaag gcgaagagtt cactgtaggt cagagcatta gcgttgaact
     2761 gtttgctgac gttaaaaaag ttgacgtaac tggcacctct aaaggtaaag gtttcgcagg
     2821 taccgttaag cgctggaact tccgtaccca ggacgctact cacggtaact ccttgtctca
     2881 ccgcgttccg ggttctatcg gtcagaacca gactccgggc aaagtgttca aaggcaagaa
     2941 aatggcaggt cagatgggta acgaacgtgt aaccgttcag agccttgacg tagtacgcgt
     3001 tgacgctgag cgcaacctgc tgctggttaa aggcgctgtc ccgggtgcaa ccggtagcga
     3061 cctgatcgtt aaaccagctg tgaaggcgta aggagatagc aatggaatta gtattgaaag
     3121 acgcgcagag cgcgctgact gtttccgaaa ctaccttcgg tcgtgatttc aacgaagcgc
     3181 tggttcacca ggttgttgtt gcttatgcag ctggtgctcg tcagggtact cgtgctcaga
     3241 agactcgtgc tgaagtaact ggttccggta aaaaaccgtg gcgccagaaa ggcaccggcc
     3301 gtgcgcgttc tggttctatc aagagcccga tctggcgttc tggtggcgtg acctttgctg
     3361 ctcgtccgca ggaccacagt caaaaagtta acaagaagat gtaccgcggc gcgctgaaaa
     3421 gcatcctgtc cgaactggta cgtcaggatc gtctgatcgt tgtcgagaag ttctctgtag
     3481 aagcgccgaa aactaagctg ctggcacaga aactgaaaga catggctctg gaagatgtgc
     3541 tgatcatcac cggtgagctg gacgagaacc tgttcctggc tgcgcgcaac ctgcacaagg
     3601 ttgacgtacg cgatgcaact ggtatcgacc cggttagcct gatcgccttc gacaaagtcg
     3661 taatgactgc tgatgctgtt aagcaagttg aggagatgct ggcatgattc gtgaagaacg
     3721 tctgctgaag gtgctgcgtg caccgcacgt ttctgaaaaa gcgtctactg cgatggaaaa
     3781 atccaacacc atcgtactca aagttgctaa agacgcgacc aaagcagaaa tcaaagctgc
     3841 tgtgcagaaa ctgtttgaag tcgaagtcga agtcgttaac accctggtag ttaaagggaa
     3901 agttaaacgt cacggacagc gtatcggtcg tcgtagcgac tggaaaaaag cttacgtcac
     3961 cctgaaagaa ggccagaatc tggacttcgt tggcggcgct gagtaagtcg gaggagtaat
     4021 acaatggcag ttgttaaatg taaaccgaca tctccgggtc gtcgccacgt agttaaagtg
     4081 gttaaccctg agctgcacaa gggcaaacct tttgctccgt tgctggaaaa aaacagcaaa
     4141 tccggtggtc gtaacaacaa tggccgtatc accactcgtc atatcggtgg tggccacaag
     4201 caggcttacc gtattgttga cttcaaacgc aacaaagacg gtatcccggc agttgttgaa
     4261 cgtcttgagt acgatccgaa ccgttccgcg aacatcgcgc tggttctgta caaagacggt
     4321 gaacgccgtt acatcctggc ccctaaaggc ctgaaagctg gcgaccagat tcagtctggc
     4381 gttgatgctg caatcaaacc aggtaacacc ctgccgatgc gcaacatccc ggttggttct
     4441 actgttcata acgtagaaat gaaaccaggt aaaggcggtc agctggcacg ttccgctggt
     4501 acttacgttc agatcgttgc tcgtgatggt gcttatgtca ccctgcgtct gcgttctggt
     4561 gaaatgcgta aagtagaagc agactgccgt gcaactctgg gcgaagttgg caatgctgag
     4621 catatgctgc gcgttctggg taaagcaggt gctgcacgct ggcgtggtgt tcgtccgacc
     4681 gttcgcggta ccgcgatgaa cccggtagac cacccacatg gtggtggtga aggtcgtaac
     4741 tttggtaagc acccggtaac tccgtggggc gttcagacca aaggtaagaa gacccgcagc
     4801 aacaagcgta ctgataaatt catcgtacgt cgccgtagca aataatttta gaggataagc
     4861 catgccacgt tctctcaaga aaggtccttt tattgacctg cacttgctga agaaggtaga
     4921 gaaagcggtg gaaagcggag acaagaagcc cctgcgcact tggtcccgtc gttcaacgat
     4981 ctttcctaac atgatcggtt tgaccatcgc tgtccataat ggtcgtcagc acgttccggt
     5041 atttgtaacc gacgaaatgg tcggtcacaa actgggtgaa ttcgcaccga ctcgtactta
     5101 tcgcggccac gctgctgata aaaaagcgaa gaagaaataa ggtaggagga agagatggaa
     5161 actatcgcta aacatcgcca tgctcgttct tctgctcaga aggttcgcct tgttgctgac
     5221 ctgattcgcg gtaagaaagt gtcgcaggct ctggatattt tgacctacac caacaagaaa
     5281 gcggctgtac tggtcaagaa agttctggaa tctgccattg ctaacgctga acacaacgat
     5341 ggcgctgaca ttgacgatct gaaagttacg aaaattttcg tagacgaagg cccgagcatg
     5401 aagcgcatta tgccgcgtgc aaaaggtcgt gcagatcgca tcctgaagcg caccagccac
     5461 atcactgtgg ttgtgtccga tcgctgagac tctggagact agcaatgggt cagaaagtac
     5521 atcctaatgg tattcgcctg ggtattgtaa aaccatggaa ctctacctgg tttgcgaaca
     5581 ccaaagaatt cgctgacaac ctggacagcg attttaaagt acgtcagtac ctgactaagg
     5641 aactggctaa agcgtccgta tctcgtatcg ttatcgagcg tccggctaag agcatccgtg
     5701 taaccattca cactgctcgc ccgggtatcg ttatcggtaa aaaaggtgaa gacgtagaaa
     5761 aactgcgtaa ggtcgtagcg gacatcgctg gcgttcctgc acagatcaac atcgccgaag
     5821 ttcgtaagcc tgaactggac gcaaaactgg ttgctgacag catcacttct cagctggaac
     5881 gtcgcgttat gttccgtcgt gctatgaagc gtgctgtaca gaacgcaatg cgtctgggcg
     5941 ctaaaggtat taaagttgaa gttagcggcc gtctgggcgg cgcggaaatc gcacgtaccg
     6001 aatggtaccg cgaaggtcgc gtaccgctgc acactctgcg tgctgacatc gactacaaca
     6061 cctctgaagc gcacaccact tacggtgtaa tcggcgttaa agtgtggatc ttcaaaggcg
     6121 agatcctggg tggtatggct gctgttgaac aaccggaaaa accggctgct cagcctaaaa
     6181 agcagcagcg taaaggccgt aaataaggag cgtcgctgat gttacaacca aagcgtacaa
     6241 aattccgtaa aatgcacaaa ggccgtaacc gcggtctggc gcagggtacg gatgttagct
     6301 tcggcagctt cggtctgaaa gctgttggcc gtggtcgtct gactgcacgt cagatcgaag
     6361 cagcacgtcg tgctatgacc cgtgcagtta agcgtcaagg taagatctgg atccgtgtgt
     6421 tcccggacaa accgatcact gaaaagccgc tggcagtgcg tatgggtaaa ggtaaaggta
     6481 acgtggagta ttgggttgcc ttgattcagc cgggtaaagt cctgtatgaa atggacggtg
     6541 ttccggaaga gctggcccgt gaagcattca agctggcagc agcgaaactg ccgattaaaa
     6601 ccacctttgt aactaagacg gtgatgtaat gaaagcaaaa gagctgcgtg agaagagcgt
     6661 tgaagagctg aacaccgagc tgctgaacct gctgcgtgag cagttcaacc tgcgtatgca
     6721 ggctgcaagt ggccagctgc aacagtctca cctgttgaag caagtgcgtc gcgatgtcgc
     6781 acgcgttaag actttactga acgagaaggc gggtgcgtaa tgaccgataa aatccgtact
     6841 ctgcaaggtc gcgttgttag cgacaaaatg gagaaatcca ttgttgttgc tatcgaacgt
     6901 tttgtgaaac acccgatcta cggtaaattc atcaagcgta cgaccaaact gcacgtacat
     6961 gacgagaaca acgaatgcgg tatcggtgac gtggttgaaa tccgcgaatg ccgtccgctg
     7021 tccaagacta aatcctggac gctggttcgc gttgtagaga aagcggttct gtaatacagt
     7081 acactctctc gatacgaata aacggctcag aaatgagccg tttatttttt ctacccatat
     7141 ccttgaagcg gtgttataat gccgcgccct cgatatgggg atttttaacg acctgatttt
     7201 cgggtctcag tagtagttga cattagcgga gcactaaaat gatccaagaa cagactatgc
     7261 tgaacgtcgc cgacaactcc ggtgcacgtc gcgtaatgtg tatcaaggtt ctgggtggct
     7321 cgcaccgtcg ctacgcaggc gtaggcgata tcatcaagat caccatcaaa gaagcaattc
     7381 cgcgtggtaa ggtcaaaaaa ggtgatgtgc tgaaggcggt agtggtgcgc accaagaagg
     7441 gtgttcgtcg cccggacggt tctgtcattc gcttcgatgg taatgcttgt gttcttctga
     7501 acaacaacag cgagcagcct atcggtacgc gtatttttgg gccggtaact cgtgagcttc
     7561 gtagtgagaa gttcatgaaa attatctctc tggcaccaga agtactctaa ggagcgaatc
     7621 atggcagcga aaatccgtcg tgatgacgaa gttatcgtgt taaccggtaa agataaaggt
     7681 aaacgcggta aagttaagaa tgtcctgtct tccggcaagg tcattgttga aggtatcaat
     7741 ctggttaaga aacatcagaa gccggttccg gccctgaacc aaccgggtgg catcgttgaa
     7801 aaagaagccg ctattcaggt ttccaacgta gcaatcttca atgcgacaac cggcaaggct
     7861 gaccgtgtag gctttagatt cgaagacggt aaaaaagtcc gtttcttcaa gtctaacagc
     7921 gaaactatca agtaatttgg agtagtacga tggcgaaact gcatgattac tacaaagacg
     7981 aagtagttaa aaaactcatg actgagttta actacaattc tgtcatgcaa gtccctcggg
     8041 tcgagaagat caccctgaac atgggtgttg gtgaagcgat cgctgacaaa aaactgctgg
     8101 ataacgcagc agcagacctg gcagcaatct ccggtcaaaa accgctgatc accaaagcac
     8161 gcaaatctgt tgcaggcttc aaaatccgtc agggctatcc gatcggctgt aaagtaactc
     8221 tgcgtggcga acgcatgtgg gagttctttg agcgcctgat cactattgct gtacctcgta
     8281 tccgtgactt ccgtggcctg tccgctaagt ctttcgacgg tcgtggtaac tacagcatgg
     8341 gtgtccgtga gcagatcatc ttcccagaaa tcgactacga taaagtcgac cgcgttcgtg
     8401 gtttggatat taccattacc actactgcga aatctgacga agaaggccgc gctctgctgg
     8461 ctgcctttga cttcccgttc cgcaagtaag gtagggttac taaatggcta agcaatcaat
     8521 gaaagcacgc gaagtaaaac gcgtagcttt agctgataaa tacttcgcga aacgcgctga
     8581 actgaaagcg atcatctctg atgtgaacgc ttccgacgaa gatcgttgga acgctgttct
     8641 caagctgcag actctgccgc gtgattccag cccgtctcgt cagcgtaacc gctgccgtca
     8701 aacaggtcgt ccgcatggtt tcctgcggaa gttcgggttg agccgtatta aggtccgtga
     8761 agccgctatg cgcggtgaaa tcccgggtct gaaaaaggct agctggtaat tgtcaccaat
     8821 tgaatcacgg gagtaaagac agatgagcat gcaagatccg atcgcggata tgctgacccg
     8881 tatccgtaac ggtcaggccg cgaacaaagc tgcggtcacc atgccttcct ccaagctgaa
     8941 agtggcaatc gccaacgtgc tgaaggaaga aggttttatt gaagatttta aagttgaagg
     9001 cgacaccaag cctgaactgg aacttactct gaagtatttc cagggcaaag ctgttgtaga
     9061 aagcattcag cgtgtcagcc gcccaggtct gcgcatctat aaacgtaaag atgagctgcc
     9121 gaaagttatg gcgggtctgg gtatcgcagt tgtttctacc tctaaaggtg ttatgactga
     9181 tcgtgcagcg cgccaggctg gtcttggtgg cgaaattatc tgctacgtag cctaatcgga
     9241 ggaaaaaatg tctcgtgttg ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa
     9301 aatcaacggt caggttatta cgatcaaagg taaaaacggc gagctgactc gtactctcaa
     9361 cgatgctgtt gaagttaaac atgcagataa taccctgacc ttcggtccgc gtgatggtta
     9421 cgcagacggt tgggcacagg ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg
     9481 tgttaccgaa ggcttcacta agaagctgca gctggttggt gtaggttacc gtgcagcggt
     9541 taaaggcaat gtgattaacc tgtctctggg tttctctcat cctgttgacc atcagctgcc
     9601 tgcgggtatc actgctgaat gtccgactca gactgaaatc gtgctgaaag gcgctgataa
     9661 gcaggtgatc ggccaggttg cagcggatct gcgcgcctac cgtcgtcctg agccttacaa
     9721 aggcaagggt gttcgttacg ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta
     9781 aggtaacact atggataaga aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa
     9841 gctccaggag ctgggcgcaa ctcgcctggt ggtacatcgt accccgcgtc acatttacgc
     9901 acaggtaatt gcaccgaacg gttctgaagt tctggtagct gcttctactg tagaaaaagc
     9961 tatcgctgaa caactgaagt acaccggtaa caaagacgcg gctgcagctg tgggtaaagc
    10021 tgtcgctgaa cgcgctctgg aaaaaggcat caaagatgta tcctttgacc gttccgggtt
    10081 ccaatatcat ggtcgtgtcc aggcactggc agatgctgcc cgtgaagctg gccttcagtt
    10141 ctaaggtaga ggtgtaagat ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag
    10201 ctgatcgcgg taaaccgcgt atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca
    10261 gctctgactg tagttggcga tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt
    10321 gaagttccag cagcgatcca gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc
    10381 gcgctgaata acggcactct gcaacaccct gttaaaggtg ttcacacggg ttctcgcgta
    10441 ttcatgcagc cggcttccga gggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt
    10501 ctggaagtcg ctggggttca taacgttctg gctaaagcgt atggttccac caacccgatt
    10561 aacgtggttc gtgcaactat cgatggcctg gaaaatatga attctccaga aatggtcgct
    10621 gccaagcgtg gtaaatccgt tgaagaaatt ctggggaaat aaaccatggc aaagactatt
    10681 aaaattactc aaacccgcag tgcaatcggt cgtctgccga aacacaaggc aacgctgctt
    10741 ggcctgggtc tgcgtcgtat tggtcacacc gtagagcgcg aggatactcc tgctattcgc
    10801 ggtatgatca acgcggtttc cttcatggtt aaagttgagg agtaagagat gcgtttaaat
    10861 actctgtctc cggccgaagg ctccaaaaag gcgggtaaac gcctgggtcg tggtatcggt
    10921 tctggcctcg gtaaaaccgg tggtcgtggt cacaaaggtc agaaatctcg ttctggcggt
    10981 ggcgtacgtc gcggtttcga gggtggtcag atgcctctgt accgtcgtct gccgaaattc
    11041 ggcttcactt ctcgtaaagc agcgattaca gccgaagttc gtctgtctga cctggctaaa
    11101 gtagaaggcg gtgttgtaga cctgaacacg ctgaaagcgg ctaacattat cggtatccag
    11161 atcgagttcg cgaaagtgat cctggctggc gaagtaacga ctccggtaac tgttcgtggc
    11221 ctgcgtgtta ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa
    11281 taagtagcag atggctaaac aaccgggatt agattttcaa agtgccaaag gtggcttagg
    11341 cgagctgaaa cgcagactgc tgtttgttat cggtgcgctg attgtgttcc gtattggctc
    11401 ttttattccg atccctggta ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg
    11461 aggcaccatc attgagatgt ttaacatgtt ctctggtggt gctctcagcc gtgcttctat
    11521 ctttgctctg gggatcatgc cgtatatttc ggcgtcgatc attatccagc tactgacggt
    11581 ggttcatcca acgttggcag aaattaagaa agaaggggag tctggtcgtc gtaagatcag
    11641 ccagtacacc cgctacggta ctctggtgct ggcaatattc cagtcgatcg gtattgctac
    11701 tggtctgccg aatatgcctg gtatgcaagg cctggtgatt aacccgggct ttgcattcta
    11761 cttcaccgct gttgtaagtc tggtcacagg aaccatgttc ctgatgtggt tgggcgaaca
    11821 gatcactgaa cgaggtatcg gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc
    11881 gggactcccg ccagccattg cccatactat cgagcaagcg cgtcaaggcg acctgcactt
    11941 cctcgtgttg ctgttggttg cagtattagt atttgcagtg acgttctttg ttgtatttgt
    12001 tgagcgtggt caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt
    12061 ctatgctgca cagagcacac atttaccgct gaaagtgaat atggcggggg taatcccggc
    12121 aatcttcgct tccagtatta ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg
    12181 tactggttgg aactggctga caacaatttc gctgtatttg cagcctgggc aaccgcttta
    12241 tgtgttactc tatgcgtctg caatcatctt cttctgtttc ttctacacgg cgttggtttt
    12301 caacccgcgt gaaacagcag ataacctgaa gaagtccggt gcatttgtac caggaattcg
    12361 tccgggagag caaacggcga agtatatcga taaagtaatg acccgcctga ccttggttgg
    12421 tgcgctgtac attaccttta tctgcctgat cccggagttc atgcgtgatg caatgaaagt
    12481 accgttctac ttcggtggga cctcactgct tatcgttgtt gtcgtgatta tggactttat
    12541 ggctcaagtg caaactctga tgatgtccag tcagtatgag tctgcattga agaaggcgaa
    12601 cctgaaaggc tacggccgat aattggtcgc ccgagaagtt acggagagta aaaatgaaag
    12661 ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat cgttaagcgt gatggtgtca
    12721 tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg ccaaggctga ttttttcgca
    12781 tatttttctt gcaaagttgg gttgagctgg ctagattagc cagccaatct tttgtatgtc
    12841 tgtgcgtttc catttgagta tcctgaaaac gggcttttca gcatggaacg tacatattaa
    12901 atagtaggag tgcatagtgg cccgtatagc aggcattaac attcctgatc ataagcatgc
    12961 cgtaatcgca ttaacttcga tttatggcgt cggcaagacc cgttctaaag ccatcctggc
    13021 tgcagcgggt atcgctgaag atgttaagat cagtgagctg tctgaaggac aaatcgacac
    13081 gctgcgtgac gaagttgcca aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat
    13141 gagcatcaag cgcctgatgg atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct
    13201 cccggttcgc ggtcagcgta ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc
    13261 gatcaagaaa taatcggggt gattgaataa tggcaaaggc accaattcgt gcacgtaaac
    13321 gtgtaagaaa acaagtctct gacggcgtgg ctcatatcca tgcttctttc aacaacacca
    13381 tcgtgactat cactgatcgt cagggtaacg cgttgggttg ggcaacagcc ggtggttccg
    13441 gtttccgtgg ttctcgcaaa tccactccgt ttgcagcaca ggttgcagca gagcgttgcg
    13501 ctgacgtcgt gaaagaatac ggcatcaaga atctggaagt tatggttaaa ggtccgggtc
    13561 caggccgcga atctactatt cgtgctctga acgccgcagg tttccgcatc actaacatta
    13621 ctgatgtgac tccgatccct cataacggtt gtcgtccgcc gaaaaaacgt cgcgtataac
    13681 gcctcgtttt ccaggtttgt tggagaaaga aaatggcaag atatttgggt cctaagctca
    13741 agctgagccg tcgtgagggc accgacttat tccttaagtc tggcgttcgc gcgatcgata
    13801 ccaagtgtaa aattgaacaa gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg
    13861 actatggtgt gcagttgcgt gaaaagcaaa aagttcgccg tatctatggt gtgctggagc
    13921 gtcagttccg taactactac aaagaagcag cacgtctgaa aggcaacacc ggtgaaaacc
    13981 tgttggctct gctggaaggt cgtctggaca acgttgtata ccgtatgggc ttcggtgcca
    14041 ctcgtgcaga agcacgtcag ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg
    14101 ttaacatcgc ttcttatcag gttagtccga atgacgttgt aagcattcgt gagaaagcga
    14161 agaagcagtc tcgcgtgaaa gccgctctgg agctggctga gcagcgtgaa aagccaacct
    14221 ggctggaagt tgatgctggc aagatggaag gtacgtttaa gcgtaagccg gagcgttctg
    14281 atctgtctgc ggacattaac gaacacctga tcgtcgagct ttactccaag taaagcttag
    14341 taccaaagag aggacacaat gcagggttct gtgacagagt ttctaaaacc gcgcctggtt
    14401 gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc
    14461 tttggccata ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat gccgggttgc
    14521 gcggtgaccg aggttgagat tgatggtgta ctacatgagt acagcaccaa agaaggcgtt
    14581 caggaagata tcctggaaat cctgctcaac ctgaaagggc tggcggtgag agttcagggc
    14641 aaagatgaag ttattcttac cttgaataaa tctggcattg gccctgtgac tgcagccgat
    14701 atcacccacg acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc
    14761 gatgagaacg cgtctattag catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg
    14821 gcttctaccc gaattcattc ggaagaagat gagcgcccaa tcggccgtct gctggtcgac
    14881 gcatgctaca gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag
    14941 cgtaccgacc tggacaagct ggtcatcgaa atggaaacca acggcacaat cgatcctgaa
    15001 gaggcgattc gtcgtgcggc aaccattctg gctgaacaac tggaagcttt cgttgactta
    15061 cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag agttcgatcc gatcctgctg
    15121 cgccctgttg acgatctgga attgactgtc cgctctgcta actgccttaa agcagaagct
    15181 atccactata tcggtgatct ggtacagcgt accgaggttg agctccttaa aacgcctaac
    15241 cttggtaaaa aatctcttac tgagattaaa gacgtgctgg cttcccgtgg actgtctctg
    15301 ggcatgcgcc tggaaaactg gccaccggca agcatcgctg acgagtaacc ggatcacagg
    15361 ttaaggtttt actgagaagg ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa
    15421 ccgcaacagc agccatcgcc aggctatgtt ccgcaatatg gcaggttcac tggttcgtca
    15481 tgaaatcatc aagacgactc tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct
    15541 gattactctt gccaagactg atagcgttgc taatcgtcgt ctggcattcg cccgtactcg
    15601 tgataacgag atcgtggcaa aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc
    15661 cggtggttac actcgtattc tgaagtgtgg cttccgtgca ggcgacaatg cgccgatggc
    15721 ttacatcgag ctggttgatc gttcagagaa agcagaagct gctgcagagt aatctgaagc
    15781 aacgtaaaaa aacccgcccc ggcgggtttt tttatacccg tagcatcccc acttatctac
    15841 aatagctgta ctctttttgt tcatcccctg gagtatttat gtggttactt gaccagtggg
    15901 cagagcgtca tatagcagaa gcgcaagcga aaggtgagtt tgataacctg gtaggtagcg
    15961 gcgaaccatt gatactggat gatgattctc atgtgccacc ggaattacgt gcggggtatc
    16021 gcttgctgaa gaatgctggt tgcttaccgc cagaacttga gcataggagg gaagcaatac
    16081 agcttctgga tattctcaaa ggtatccgtc atgatgatcc gcaatatcaa gaggttagtc
    16141 gtcgattgtc attactggaa ttgaagctgc gacaagctgg attgagtacc gattttttac
    16201 gcggcgatta tgctgacaag ttgttgaaca aaatcaacga taactagtgg agtatgtatg
    16261 tatcgcattg gtgagctggc aaaaatggcg gaagtaacac ccgacacgat tcgttattac
    16321 gaaaagcagc agatgatgga gcatgaagta cgcaccgaag gtgggtttcg cctgtatacc
    16381 gaaagcgatc tccagcgatt gaaatttatc cgccatgcca gacaactagg tttcagtctg
    16441 gagtcgatcc gcgagttgct gtcgatccgt atcgatcctg aacaccatac ctgtcaggag
    16501 tcaaaaggca ttgtgcagga aagattgcag gaagtcgaag ctcgtatagc cgagttgcag
    16561 agtatgcagc gttccttgca acgccttaat gatacctgtt gtgggaccgc ccatagcagt
    16621 gtttattgct cgattcttga agctcttgaa caaggggcga gtggcgttaa gagtggctgt
    16681 tgattttttg cactggcagg attacactcg cgccgttaaa taaccaattg gagtttttat
    16741 gagccgatat cagcatacta aagggcagat aaaggataat gcgatagaag cattactaca
    16801 tgatccctta ttccgacagc gagtagagaa aaataagaaa gggaaaggca gttacatgcg
    16861 aaaaggaaaa catggcaatc gggggaactg ggaggccagt ggcaagaaag taaatcactt
    16921 ttttaccact ggccttctgc tttcagacgc ttgttaagag cggttattct gctctttcag
    16981 caaatcacga atttctgtca gtaatacttc ttctttagtt ggtgcaggtg ttgctgctgc
    17041 tggttcttct tttttccgat tcagtttgtt gattagctta atcgccataa agatggcaaa
    17101 ggccactatc agaaaatcaa agacgttttg aatgaagaca ccgtaatgca tcacaacagc
    17161 agggatatcc ccttgggcct cgcgtagcgt gacagcaaac tgtttaaagt cgatcccgcc
    17221 aattaataag cccagaggtg gcatgatgat atcggcaacc agtgaagata caatcttccc
    17281 gaatgccgct ccgataatga cacccactgc caaatccacc acgttcccgc gcatcgcaaa
    17341 ttcgcgaaat tctttaataa tgctcatgtt attctcccta tgaagccgac aaccataagt
    17401 ctaacaaat
//
LOCUS       DACADA010000095        17306 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.097, whole genome shotgun sequence.
ACCESSION   DACADA010000095 DACADA010000000
VERSION     DACADA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.097"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(57..788)
                     /gene="rlmB"
                     /locus_tag="ISB17_003104"
     CDS             complement(57..788)
                     /gene="rlmB"
                     /locus_tag="ISB17_003104"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP1981003.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(879..3320)
                     /gene="rnr"
                     /locus_tag="ISB17_003105"
     CDS             complement(879..3320)
                     /gene="rnr"
                     /locus_tag="ISB17_003105"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP1981004.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3359..3784)
                     /gene="nsrR"
                     /locus_tag="ISB17_003106"
     CDS             complement(3359..3784)
                     /gene="nsrR"
                     /locus_tag="ISB17_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAP1981005.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3989..5287)
                     /gene="purA"
                     /locus_tag="ISB17_003107"
     CDS             complement(3989..5287)
                     /gene="purA"
                     /locus_tag="ISB17_003107"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP1981006.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5391..5588)
                     /locus_tag="ISB17_003108"
     CDS             complement(5391..5588)
                     /locus_tag="ISB17_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAP1981007.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5670..6674)
                     /gene="hflC"
                     /locus_tag="ISB17_003109"
     CDS             complement(5670..6674)
                     /gene="hflC"
                     /locus_tag="ISB17_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAP1981008.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6677..7936)
                     /gene="hflK"
                     /locus_tag="ISB17_003110"
     CDS             complement(6677..7936)
                     /gene="hflK"
                     /locus_tag="ISB17_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAP1981009.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8022..9302)
                     /gene="hflX"
                     /locus_tag="ISB17_003111"
     CDS             complement(8022..9302)
                     /gene="hflX"
                     /locus_tag="ISB17_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP1981010.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9378..9686)
                     /gene="hfq"
                     /locus_tag="ISB17_003112"
     CDS             complement(9378..9686)
                     /gene="hfq"
                     /locus_tag="ISB17_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAP1981011.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9772..10722)
                     /gene="miaA"
                     /locus_tag="ISB17_003113"
     CDS             complement(9772..10722)
                     /gene="miaA"
                     /locus_tag="ISB17_003113"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP1981012.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10715..12562)
                     /gene="mutL"
                     /locus_tag="ISB17_003114"
     CDS             complement(10715..12562)
                     /gene="mutL"
                     /locus_tag="ISB17_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP1981013.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12572..13903)
                     /gene="amiB"
                     /locus_tag="ISB17_003115"
     CDS             complement(12572..13903)
                     /gene="amiB"
                     /locus_tag="ISB17_003115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAP1981014.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13922..14383)
                     /gene="tsaE"
                     /locus_tag="ISB17_003116"
     CDS             complement(13922..14383)
                     /gene="tsaE"
                     /locus_tag="ISB17_003116"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP1981015.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14355..15902)
                     /gene="nnr"
                     /locus_tag="ISB17_003117"
     CDS             complement(14355..15902)
                     /gene="nnr"
                     /locus_tag="ISB17_003117"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAP1981016.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15901..17040
                     /gene="queG"
                     /locus_tag="ISB17_003118"
     CDS             15901..17040
                     /gene="queG"
                     /locus_tag="ISB17_003118"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP1981017.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17023..17076)
                     /locus_tag="ISB17_003119"
     CDS             complement(17023..17076)
                     /locus_tag="ISB17_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981018.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3938 a         4925 c         4330 g         4113 t
ORIGIN      
        1 gctatagaca aaaaaaaacc atccaaatct ggatggcttt tcataattca gaggaactag
       61 ctgcgctgac gaaccgcttc aaataagcaa attccggttg caaccgaaac gttcagggaa
      121 gaaacacttc ctgccattgg gatgctgatc aactcatcgc aatgttcacg ggtcaggcga
      181 cgcatacctt caccttccgc gcccatcacc agcgccaggc gtccggtcat tttgctctga
      241 tagagtgtat gatccgcctc gcctgccgta ccgacgatcc agatattctc ttcctgcaac
      301 atacgcatgg tgcgcgccag attagtcacc cgaatcagcg gaacgctttc tgccgcacca
      361 caggccactt ttttcgccgt ggcgttgagc tgtgcggagc gatctttcgg cacaatcacc
      421 gcatgaacac cagcggcgtc cgcgctacgc aggcacgcgc cgaggttgtg cggatcggtt
      481 acgccgtcga ggatcagcag gaacggttga tcgagcgaag cgatcagatc cggcagatcg
      541 ttttcctgat actgccgtcc tggcttcaca cgagcgataa tgccctgatg cacggcaccg
      601 tcgcttttct cgtcgagata ttggcggttt gccaactgga taaccacgcc ctgagactca
      661 agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat aaagacttcc
      721 tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg catggatgcc gtaaatcatt
      781 tcgctcatta atgtactcgt tgtttacgtt ttgccgggag cgccacgctt gcccggccta
      841 cggataattc accctcttct gtttgaagag ggtaatgatc actctgccac ttttttcttc
      901 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga tggctttttc
      961 gcttttctcg cgtctttctt cgctgctttc ggcttcggtt ttttctcgcc acggaaggcg
     1021 ctgtctggct caaagttgac ctttttgccg acctgacgcc gcttgccgcc ttttttgcct
     1081 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttcg
     1141 ctggagatca ggctaaagtc gattttgcgc tcgtccatat taaccgcttc gacgcgaact
     1201 tccacgcgat cgcccaggcg ataagtctga ccgctggatt cacccatcag gcgttgccct
     1261 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
     1321 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
     1381 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcaga cacatcgcgc
     1441 gttgcttcat cggcacgacg ttccgccatc gaacagtgct gacccagttg cagcatctct
     1501 tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc ctgctctttc
     1561 gccagcagat atttaatggc gcggtgcaac gtcaggtcag gataacgacg aatcggcgaa
     1621 gtaaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc cggatcgtaa
     1681 atcgcctgtt tcatcgagcg caaaagcatg gtttgcagca tttctgcatc aggacgatcg
     1741 gcaaccgatt ccagtaactc tgcatagtcg cgcggttccg gcttgttacc gcccggcaat
     1801 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
     1861 tcgtgaatac ggaacagtgc cggttctttg gctttctcaa cgaaacgcgc cgccgagata
     1921 ttcgccagaa tcatgcactc ttcaattaac ttgtgggcgt cgttacgctg agtctgttcg
     1981 atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
     2041 atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg taactcttcg
     2101 agatgcttaa ccagcggcgc atactgctcg cgcagatcct gatcgccttg cagtatatgc
     2161 cagactttgg tgtaggtcag acgcgcgtga gaactcatca ccgcttcgta gaatttgtag
     2221 ccagtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
     2281 tgcgggttga gcgagcacag gccgttagag agcacttccg gcagcatcgg gataacctgc
     2341 gaagggaagt acaccgacgt accacggtta cgcgcttctc tgtccagcgg tgttggcgga
     2401 cggacatagt aactgacgtc ggcaattgcg acccacaaac gccagccacc gccgcgtttt
     2461 ttctcacagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
     2521 ggtaaatcgc gcagatcgac acggcccgct tttgcttctt ccggcacttc ttctttcagc
     2581 ccggcaacct gttgctcaac agcctgcggc cagatgtacg gaatttcatg ggtacgcaga
     2641 gcaatatcaa ccgccatgcc ggtgcccata ttatcgccca gcacttcgac gattttaccc
     2701 accgctttgg tacggcgagt cgggcgttga gtcagttcaa ccaccaccac aaagcccatc
     2761 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg gctatcgtca
     2821 ggaaccacaa agccgacgcc cgcttcggtg aagtagcgac caacaatctg gctggttttt
     2881 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc gcccagcggc
     2941 tgagccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga gagatacaaa
     3001 tcatctttac gcccttcaac tcgcagaaag ccgtagccat cacggtggcc aataacggta
     3061 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacggcgagt gaagaccagt
     3121 tgaccatcgc gctccatcgc gcgcaggcgt cgacgcaggc cttcaagctg ctcttcgcct
     3181 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
     3241 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
     3301 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
     3361 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
     3421 agttatccag ttccgtcaga aaactttgca cggccttaga aagcgcctgt ttcaaccgac
     3481 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
     3541 cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccaagcga atgccgccat
     3601 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
     3661 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
     3721 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
     3781 gcacatcaac ctcaaaaggg aaatcgggga aaaatacatt tatattgatg ataaaccagc
     3841 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaaacgag gatttaacac
     3901 tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt tagcccggca
     3961 ggcaaaatat ctgccatgcg taccagaatt acgcgtcgaa cgggtcgcgc agaatcatgg
     4021 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccggtc agctcttcaa
     4081 tgcgcttgat gtagttcagt gccgcctgcg gcaggccact acgatctttc acgccgaagg
     4141 tggattcaga ccagcccggc atggtttcgt aaatcggctc tagacctttc cagtcgtcag
     4201 ctgccagcgg agtggtagtc acttcacgac catccggcat acggtaagcc acgcagagtt
     4261 taacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca gacagggagt
     4321 tcaactgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgac
     4381 ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
     4441 gttcggtcgg gaacggacct gcccccacac gagtggagta agctttgagg atacccagaa
     4501 cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
     4561 tggaagaagt tacgtacgga tatgtaccgt ggtcgatatc cagcagcgta ccctgcgcac
     4621 cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
     4681 caaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
     4741 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
     4801 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
     4861 ctactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
     4921 cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc agcggacatg
     4981 cttcggacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
     5041 ctttcatcag cgcagccgga gacagcacaa caccgttacc gatgatgctg gttacattct
     5101 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
     5161 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
     5221 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
     5281 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
     5341 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
     5401 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
     5461 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
     5521 taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag ccagattgtc
     5581 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
     5641 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
     5701 tagcggaaga aatcgctgtc cgggctcatg accatcacgt cctgattgcc agagaagctg
     5761 ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt actgaatgca
     5821 tcagcaaaca gtttggctgc ttctgcatcg ccttcaccac gcatgatgcg gccctgacgc
     5881 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag tttttccgct
     5941 tcttcctgac cttgtgaacg gtgacgacgt gctaccgctt cacgctcggc gcgcatacgg
     6001 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttaattcg cacatcgaca
     6061 acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt gcccttcgtc
     6121 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgctgcagg ggtagtaact
     6181 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
     6241 ccacgggaat cggtgacgat atctttcacg tccaggcgac caatttcaga acgcagacgg
     6301 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
     6361 agatagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac gatcaggtct
     6421 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
     6481 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc ataaaccaga
     6541 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgctcacct
     6601 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
     6661 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
     6721 ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct ccactggttg
     6781 tggaggaaga ggctggcggc agacgcagca gattgctggc accgttatcg ctcttcgccg
     6841 caggggcgtt accacctttc agcatctggt ctaatggcag aaccatcagg ttgccacctt
     6901 tatcgttaac cagcactttg cgggtgttgc ccaacacttt ttccatcgtc tcgatataca
     6961 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
     7021 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
     7081 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct tctgcttcac
     7141 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
     7201 cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc atgtcatacg
     7261 gacgaatcgt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
     7321 tcagaatgcg gtccatggtg tatttaccga taaccccgcg tagggcgctg tcggtagcct
     7381 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga tcggtgacgc
     7441 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
     7501 aagctgccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtca ataaacgtcg
     7561 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
     7621 cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
     7681 ccgctgcgat ggtaacgacg cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
     7741 caccgccaga tccggtgcct ttaccgccac ccagaccacc gagctttttg ctcagtttgc
     7801 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
     7861 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
     7921 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
     7981 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
     8041 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
     8101 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
     8161 cttctcagac gcccttcctg cggcggcaga cgcaatgtat gctgcgccac ctcgccggaa
     8221 agacgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg cgcagaaagc
     8281 cagacacgga tcggtttgtt ctcttcatct ctgtcaatac gcggttcgaa atcgtccagc
     8341 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcatcaat ctcttcaaga
     8401 accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcgtc aatgacgtgc
     8461 agcagcaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc caccagatcg
     8521 tgcggcaggt ggcgaataaa ccctacggta tctgccaaca cggtttcacc gacatccgca
     8581 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
     8641 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
     8701 gaaacagtag gaacatcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
     8761 actctttcca ggcgcgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
     8821 tcgagctggg tttcacccgg accacgcaaa ccgatcccgc ctttctgtct ttcaaggtgg
     8881 gtccagccac gcaccaggcg cgtagccaga tggcgcaact gcgccagctc aacctgcaac
     8941 ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
     9001 atgacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
     9061 tcaaaaagaa cgaccgaagc acccgtcgct tttacggctt ccgcaatttc aactgcttta
     9121 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
     9181 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
     9241 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
     9301 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
     9361 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
     9421 gcgctgctac catgatgata gttactgctg gtaccgccac cggcgttgtt actgtgatga
     9481 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
     9541 gtgtttttca acaggatcac gaactgatca aaagactcga tttgtccttg cagcttaata
     9601 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
     9661 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
     9721 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
     9781 gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt cactgtcaag
     9841 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
     9901 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
     9961 catctggcga taacccacgc aacgaatgga aggcaaatcc gtatgcaaat ctcctcgggc
    10021 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    10081 aattcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    10141 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    10201 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    10261 cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    10321 ctcaattctg gcccgtactt ccgggtccgc cgacggtagc ggcgacaacc cttccagcaa
    10381 tgccttgaaa tacaacatcg taccgcccac taatagtggg atccgccccg ccgcggtgat
    10441 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    10501 atcgcgaata ttcagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    10561 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    10621 taaaatttta cgcagctcaa tggctaacgc tgttttaccg gaggccgtcg gccccatcaa
    10681 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    10741 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    10801 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    10861 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    10921 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    10981 aaggcacggc cctgatggtc acatgctgcg catcggactg gaaatcaata cccaattccg
    11041 ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact tttagccgca
    11101 acggaataag cagcggttgg gcgcaaactg gcacttcacc cggcgtcaat tgtgcctgac
    11161 gcagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg cgctcaagca
    11221 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    11281 gtgcaggttc ctgcggttct ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa
    11341 gctggcgata cacttcacct tgctgtttct ggtaacctgg ctgcgcattc ggccaggggg
    11401 cagccggacg actgcctgag gagggcgcag gagtgtaacg cggagctact ggctcacgaa
    11461 ctgccggttc agcaaaatga ttgcgccccg ccgccacgcg attttccgga atggcacgcg
    11521 gtgcaggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    11581 tcagcacgcc ctgatagata aagtcatgca ccagacgcga ctgatggaaa cgcacttcgt
    11641 gtttggcagg gtgcacgttg acgtccacct gatgcgggtc gatctccaga tacaacacaa
    11701 atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc gcgtgattga
    11761 tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata ctgaatttct gccagtgcgg
    11821 gcgtggtgtg atttggatcg gccacccagc cgcgcagcgt gagatcgccg tgttgccatt
    11881 caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    11941 tttgcccgcc ttccggcact gcgcggtact ggcgtacaat tttgccgtta tgcgacaggt
    12001 tgatagtgac gtcgaaacgc gccagcgcaa tgcggcggat gatttcatcg atatggttaa
    12061 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacaggtcca
    12121 gcacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg tccatattgc
    12181 gcccttcggc ataagcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    12241 ggcgggaaac cgaactgata ctcgccagtg cctcaccgcg aaagcccagg ctgataatgg
    12301 cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc gccagtgcca
    12361 gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    12421 gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc accagttctt
    12481 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    12541 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    12601 tcaccgtact ggccgtttgt gccgttgcac cctgcggcgc agattgcatc ggatgcgcaa
    12661 ggaagtaatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    12721 tcgccagcaa acgttcttcg ctgttgttac tgataaaacc ggtttcgacc agtactgacg
    12781 gaatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt
    12841 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgctggg
    12901 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcggcc tgactgttcg
    12961 ccagcacatc acccgccccg cccagcagct ccgactgctt ctcatgctgt tccagccagc
    13021 tggccatttc gctgttggcg cgacggtttg agagcaccca tacagaggca ccggtggcac
    13081 tacggtttgg tgcggcatcg gcatgaatcg acacaaggaa attggcgttt tgcttacgtg
    13141 ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgccct
    13201 taaacatcgg atcgttattg agcaaggtac gtaatttacg cgcaatggcg atggtgacat
    13261 ttttctcccg tgtaccacca gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    13321 caatagcgat gataatttta tcgcctgtgt tagccgtcgc gcgcgctgcc ggacgcgtta
    13381 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    13441 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccattg
    13501 gcggaggcga tggcggcgca tcggcgttaa tcgtaaagac aacagtgtaa ttgctgccat
    13561 tctgccgctt caccgcttcg gttttaccgt tttcggtgag atcgaccacc agccgtagcg
    13621 tttgtgcatc tttaggcgtt ccggagcgaa tcgctttcac caggttatta ccgctaaaca
    13681 acaacggcag cccctgaatt acccccgttt gtttgatatc gagcgccacg gtgcgtttgc
    13741 tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgtt
    13801 gattaccgtt agaaacctga atatcagaga gcgtcgcggc ccccgcctgc gcgcacagca
    13861 gcagcaacgt cgctactaac caatttctga tgcgatacat catcccgcca cctttcaaag
    13921 gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg
    13981 cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac
    14041 ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga
    14101 tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgataaacca
    14161 ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct
    14221 gatgacccag agcctgtaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc
    14281 catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta
    14341 atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact
    14401 tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt
    14461 ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc cgcacaggct
    14521 gcatcataag ggctcatttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg
    14581 cccatgccgc cgctcgccat gcctgcattt ccggcatcaa taatgcctaa agcgtcagga
    14641 tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc
    14701 agacgttggg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca tcctaacaac
    14761 cgtgcggcct caccaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt
    14821 gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt
    14881 tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg
    14941 gcccattcca ggctttcggt aagagagccc atagtcagtt catgcaccat caattccggt
    15001 cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca gtactcggac taaaccagca
    15061 ccagcacgca gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca
    15121 ccaataatca ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggtatcagc
    15181 cactgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccat
    15241 ctatccagcc gcagtgagtc aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg
    15301 agcaaacctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat taccgcgcct
    15361 ggcgtagcgc cggtttcagc cagaaggccg gaagggatat caaccgccac aatcggcgca
    15421 ggatgggaat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaaa
    15481 ccggtgccga gcagcgcatc gacaatcaga tctaccgatt cgggccagac aatattcgaa
    15541 gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc
    15601 aacggtttgt cactttcctg ggccagcagc gtgacatcaa taccgatcgc tttggccagc
    15661 cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg
    15721 cgggcgtcag gataagccga acgacacacc tggaatgcgg cttcgccagc gcgaagcatc
    15781 agctcataga gtgtgagccc cagcgcatct gccgcctcgc gttctccgcg gcggatatcg
    15841 tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc
    15901 atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa
    15961 ctgggctttc agcaggtagg tattaccgat accgatctca gcgagtccga gcccaaactg
    16021 caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcgcg tcacggtatg
    16081 ctgcgtgctc gtcctcacga attattgccc ggtacgctgc gcgtgatcag cgtgcggatg
    16141 aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaatcccaa actcggctat
    16201 gttagccgtt atgcgctggg ccgtgactat cacaagcttc tgcgcaaccg actcaaaaag
    16261 ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct
    16321 gcgcctattc tcgagcgccc gttagctgaa aaagcaggtc ttggctggac aggtaagcac
    16381 tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt gctggtcgat
    16441 attccgctgc ccgtggatca accagtcgag gaaggatgcg gtaaatgcgt ggcctgtatg
    16501 acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc
    16561 tcttatctca ccatcgaact ggaaggggag atcccggaag agttgcgacc gttaatggga
    16621 aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa
    16681 ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag
    16741 ttattcgcct ggagtgaaga gaagttttta aaagtcacgg aaggttcggc gattcgccgt
    16801 ataggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc cccctgggat
    16861 gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct tgatgagcac
    16921 atagcgtggg cgattgcgca gcaaatcgag agacgaaatg cgtgcgtggt cgaagtgcaa
    16981 ttaccgaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga
    17041 ttcatccaca gcttgtgaat aaaaataaaa acgcattgca attcaagccg gaaaaaatcg
    17101 tcaagtgatc gatacgacaa aacgaaatct taatttttaa cttatctatc aataggttaa
    17161 aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacggcct gaggctgtgg
    17221 ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc
    17281 gctgtggata ttttattgag agaaga
//
LOCUS       DACADA010000096        17287 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.098, whole genome shotgun sequence.
ACCESSION   DACADA010000096 DACADA010000000
VERSION     DACADA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.098"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            6..524
                     /locus_tag="ISB17_003120"
     CDS             6..524
                     /locus_tag="ISB17_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP1981019.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            540..1079
                     /locus_tag="ISB17_003121"
     CDS             540..1079
                     /locus_tag="ISB17_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981020.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1172..2749)
                     /gene="guaA"
                     /locus_tag="ISB17_003122"
     CDS             complement(1172..2749)
                     /gene="guaA"
                     /locus_tag="ISB17_003122"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP1981021.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2818..4284)
                     /gene="guaB"
                     /locus_tag="ISB17_003123"
     CDS             complement(2818..4284)
                     /gene="guaB"
                     /locus_tag="ISB17_003123"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP1981022.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4446..5813
                     /gene="xseA"
                     /locus_tag="ISB17_003124"
     CDS             4446..5813
                     /gene="xseA"
                     /locus_tag="ISB17_003124"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP1981023.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGATLHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(5867..13948)
                     /locus_tag="ISB17_003125"
     CDS             complement(5867..13948)
                     /locus_tag="ISB17_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HAP1981024.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(14058..15050)
                     /locus_tag="ISB17_003126"
     CDS             complement(14058..15050)
                     /locus_tag="ISB17_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981025.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            complement(15106..17280)
                     /gene="sinH"
                     /locus_tag="ISB17_003127"
     CDS             complement(15106..17280)
                     /gene="sinH"
                     /locus_tag="ISB17_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HAP1981026.1"
                     /translation="MLRWKRCIMLTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHN
                     FDKWLLGGNIFYDYDFTRGHRRLGIGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPASGWDIRAEAWLPAYPQLGGKIVFEHYYGDEVALFGTDNLEKDPFAVTLGVKYQPV
                     PLIAVGTDFKAGTGDNTDTTVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADAMNEHPECVIKDTPEEAIGLEKCKWTINALVNHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPVWQYSSDKAEQGKLNTWR
                     VRLALKDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDITDSFGDNTDRNGNVLPQDNLNPQLFDANDKKVTLTNNPCTTETPCVFIAKQD
                     KEKGTVTLSSTLPGTFRWKAKAAPYDDSNYVDVTFLGADIGGLNAFIYRVGAAKSVNL
                     IGNKEPLPLNSRYRFVLWRDANKDGVFQLSEKLTDEEMKQYDYQWEFTGHSVNGNTGA
                     QANTTNVDIEIPATNKEAAAKFSAQVTDGVQGYGLQVNYSKT"
BASE COUNT         3910 a         4722 c         4392 g         4263 t
ORIGIN      
        1 gaatagtgat gaaatttaaa aaatgtcttc tgcctgtggc aatgttagcg tcattcactc
       61 tggcaggatg ccagtcaaat gctgatgatc atgccgccga tgtttatcaa accgatcaac
      121 tgaataccaa acaagaaact aaaaccgtta atattatttc cattcttccc gcaaaagttg
      181 ccgtagacaa cgcccaaaat aaacggaacg cacaagcctt cggcgcgctt attggcgctg
      241 tcgctggcgg tgttatcggc cacaacgtcg ggtctggcag caattccgga acgacggcag
      301 gtgcagttgg cggcggagct gtaggcgcgg cagcgggttc tatggtgaat gataaaacct
      361 tagtggaagg tgtttcttta acatataagg aaggcaccaa agtgtatacc tccacccaag
      421 tgggtaaaga gtgccagttt acgacaggtt tagccgttgt tattaccacg acgtataacg
      481 aaacgcgtat tcagccaaat accaaatgtc ctgaaaagag ctaataatca ggaggagtca
      541 tgaagaaagt ttttctttgc gccatcttag cctccttaag ctatccggct atcgcctcat
      601 cattgcagga tcaactctcg gctgtcgcag aagcggaaca gcaaggtaaa aatgaagagc
      661 aaaggcagca tgacgaatgg gtcgcggagc gcaacaggga aatccagcaa gagaagcaac
      721 gtcgcgcaaa cgcccaggcc gcggctaata aaagagcggc aacggcagca gcgaataaga
      781 aagctcgtca ggataaactg gacgccgaag ccactgcgga caaaaaacgc gatcaaagtt
      841 atgaagatga gctacgtagc ttagagattc agaaacaaaa actggcgctg gcgaaagaag
      901 aagcccgcgt taagcgcgaa aacgaattta tcgatcagga actgaagcac aaagctgcgc
      961 aaaccgatgt ggtgcaatct gaagctgacg caaacagaaa tatgactgaa ggcggtcgcg
     1021 atctgatgaa aagcgtgggt aaagcagaag agaataaatc agatagctgg tttaactaaa
     1081 cgatgttagt aacttcaaac ctataattct ttaggataaa aaaccctctg tagtaacaga
     1141 gggttttgtt cattcatagt gcagggtcaa atcattccca ctcaatagtt gctggtggct
     1201 tgccgctgat gtcatacacc acgcgggaaa taccattcac ttcattgata atgcggttgg
     1261 aaactcgacc gaggaaatcg tacggcagat gtgcccagtg tgcggtcata aagtcgatgg
     1321 tttcgacagc acgcagagag acaacccagt catacttacg accatcgccc attacgccaa
     1381 cggaacgtac tggcaggaag acggtgaacg cctggctgac tttgtcgtac aggtccgctt
     1441 tacgcagttc ttcaatgaag atggcgtcag cacggcgcag caggtcacaa tactctttct
     1501 tcacttcccc cagaacacga acgccaaggc ctggtcccgg gaacgggtga cggtacaaca
     1561 tgtcatacgg caggcccagc tccagaccaa tcttacgcac ttcgtctttg aacagctctt
     1621 tcagcggttc aaccaggccc atcttcatct ctttcggcag cccacccacg ttgtggtgag
     1681 atttgatgac gtgtgcttta ccggttgcag aagcagcaga ttcgataacg tcagggtaga
     1741 tggtgccctg cgccagccac ttcacgtctt ccagtttcag cgcttcttca tcgaatactt
     1801 caacgaaaac gcgcccgatg attttacgtt ttgcttccgg atcgttttcg ccagccagcg
     1861 ctgacaagaa gcgatcttct gccggtacgt gaacaatgtt cagaccaaag tgatcgccaa
     1921 acatatccag aacctgctct gcttcgttga gacgcagcag gccgttgtcg acgaatacgc
     1981 aagtcaggtt tttaccgata gcgcggtgca acagcattgc ggttacggag gaatccacgc
     2041 caccagagag gccgaggatg actttatcgt cgcctacctg ctcgcggatg cgggctacag
     2101 cgtcgtcgat aattttcgct ggcgtccaca gggcttcaca ctggcagata tcacgcacaa
     2161 aacgctccag catgcgcata ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc
     2221 catagaagcg tttttcttcg ttggccatga tggcaaacgg gcagctttcg gtgctggcta
     2281 cggtgacgaa gtcggacggg atagcggtaa ctttatcgcc gtggctcatc cagacgtcca
     2341 gcagcggttt accatctgcg gtcagagcat cttcgatacc gcgaaccagt gcgctgtcgt
     2401 ttacgacttc aacctgcgcg tagccaaatt cacgttcatt agaagcctca acgtgaccgc
     2461 ccaactgcat cgccatggtc tgcataccat agcaaacgcc gaataccggt acgcctgctt
     2521 caaagacata ctgcggcgca cgcgggctgt tttcttcagt agtactttcc gggccgccgg
     2581 aaagaataat gccgcttgga ttgaagtcac gaatttgtgc ttctgtcaca tcccacgccc
     2641 acagttcgca gtaaaccccc agttcacgca cgcggcgcgc aaccagttga gtatactggg
     2701 aaccgaagtc cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt gacgcttatt
     2761 ccgaggcaag tgaaacagat aatataaatc gcccgacatg aagtcgggcg aagagaatca
     2821 ggagcccaga cggtagttcg gggactcttt agtaatggtc acgtcgtgaa cgtggctttc
     2881 ctgaataccc gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt cgtcgatagt
     2941 accacagccg gtcaggccca tacaggagcg caggccgccc atctgctggt gaatgatctc
     3001 tttcaggcga cctttatagg ctacgcgacc ttcgatacct tccggcacca gtttgtcggc
     3061 agcgttgtcg ctctggaagt aacggtcaga agaacctttg gacatcgcgc ccagggaacc
     3121 cataccacgg taagatttgt aagaacggcc ctggtagagt tcgatttcac ccggagattc
     3181 ttcagtacct gccagcatgg aacctaccat caccgcgctt gcgccagcgg cgatagcttt
     3241 agcgatatcg cctgagaaac ggataccgcc gtcagcgata accggaatgc cggtaccttc
     3301 cagggcttct actgcatcag caacggcggt gatctgcgga acaccgacac cagtcacgat
     3361 acgcgtcgta cagatagaac cagggccgat acccacttta acggcactgc aaccggcttc
     3421 tgccagcgcg cgggcacctg cagcagttgc cacgttgccg ccgatgattt gcaggtcagg
     3481 atatttagcg cgagtttcac ggatacgctg cagaacacct tcagagtgac cgtgagagga
     3541 gtcgatcagc agaacgtcaa cgcctgcggc aaccagcgca tcaacacgct cttcgttacc
     3601 agcgcccgcg ccaaccgctg cacctacacg cagacggcct tgttcgtctt tacacgcgtt
     3661 cggtttacgt tccgctttct ggaagtcctt aacagtgatc atgccgatca ggtggaattc
     3721 atcatcaacc accagcgctt tttcaacgcg tttttcgtgc atttttgcca gcaccacttc
     3781 acgggcttcg ccttcacgca cggttaccag acgctctttc ggcgtcatgt aaacgctaac
     3841 cggctggctc aagtcggtca caaaacgcac gtcacgaccg gtgataatgc cgaccagttc
     3901 gttttcttcg gtaacaaccg ggtagccagc aaagccgtta cgctcggtca gttctttcac
     3961 ttcacgcagc gtcgtagttg gcagtacggt ctgcggatcg gttaccacgc cagattcgtg
     4021 ttttttcaca cggcgaactt cttctgcctg gcgttcaatg gacatgtttt tgtggataaa
     4081 gccgataccg ccttcctgag ccagagcaat agccaggcgc gcttccgtta cggtatccat
     4141 tgctgcggaa agcataggga tattcagacg aatagttttc gtcagctgag tgctgaggtc
     4201 agcagtattt ggcagaacgg tagaatgagc aggaacgagg agaacgtcgt caaacgtcag
     4261 agcttcttta gcgatacgta gcatgggcaa tatctcgacc agagtggtta ataaatattg
     4321 ccgcggcatt atacagagcg taaccgattg catctacccc tttttgcaaa aaatgcttgc
     4381 tatccccgga gggcgggtta ctatcgactg aataacctgc tgatttagaa tttgatctcg
     4441 ctcacatgtt accttctcaa tcccctgcaa tttttaccgt tagccgcctg aatcaaacgg
     4501 ttcgtctgct gcttgagcat gagatggggc aggtgtggat cagcggcgaa atctctaatt
     4561 ttacacaacc agcctccggt cactggtact ttacgcttaa agacgatacc gcgcaggtgc
     4621 gctgtgcgat gttccgcaac agcaaccgcc gggtcacctt ccacccacag cacgggcaac
     4681 aggttttagt tcgcgccaat attacactct acgagccgcg cggcgattat caaattatcg
     4741 tcgaaagtat gcagccggcc ggtgagggat tgctgcaaca gaagtacgaa cagcttaaag
     4801 cgaagttgca ggctgaaggt ttgttcgatc tgcaatacaa aaaatcactc ccctcccctg
     4861 cgcattgcgt tggtgtgatc acctcaaaaa ccggtgctac actacatgat attttgcatg
     4921 tgttaaaacg tcgcgatcct tctctgccgg tgatcatcta ccccaccgcc gtacaggggg
     4981 atgatgcgcc ggggcaaatc gttcgtgcca ttgaactggc gaataagcgc aacgagtgtg
     5041 acgtgttgat cgttggccgt ggcggcggtt cgctggaaga tttatggagc tttaacgacg
     5101 aacgcgtggc gcgggcgatt tttgccagtc gcattccgat agtcagcgcc gtcgggcatg
     5161 aaaccgatgt gacgattgcc gactttgttg ctgatctgcg tgcgccaacg ccgtcagccg
     5221 ccgctgaagt ggtgagccga aatcagcaag aattgctacg tcaggtgcaa tctgcccgtc
     5281 aacggctgga aatggcgatg gactattatc tcgccaatcg cacgcggcgc tttacgcaga
     5341 tccatcaccg cttgcagcaa cagcatccgc agctccggct ggcacgccag caaactatgc
     5401 tcgaacgtct gcaaaagcgg atgagctttg cgctggagaa tcagcttaaa cgcgcgggtc
     5461 agcaacagca gcgattaaca cggcaactcg tccaccaaaa tccgcagtcg cgtattcatc
     5521 gcgcgcaaac gcgcattcag caactggaat atcgtttagc agaaaccctg cgcgcacagc
     5581 ttagcgccac gcgcgaacgg tttggtaacg cggtaacaca cctcgaagcg gttagcccgc
     5641 tgtcaacgct ggcgcgcggt tatagcgtca ccagcgccgc cgatggcgca gtgttgaagc
     5701 aggtgaagca ggtgaaagtg ggcgatacgt tgaccacgcg actgggagat ggcgtggtcg
     5761 tcagtgaagt gagtgcggtg acgaaaaccc gcaagccgcg taaaaaaacc tgatagcccg
     5821 ttgcgtatca ggctttcgga acctcatatt gatagggtgg gcaaggttat tggaaacagg
     5881 ttgcaaaccc cggcgttttc gcaggctgaa tgccacagtt gagactgcca gagccttcct
     5941 caacaccaca atagaacgat ccagccttat cagtagataa atacgggaag cttatcgatg
     6001 acggccatcc caatgtgcta aagccggagt gtaatgtttg taattcggca ctgctcggca
     6061 cgttattcat actaccgcac tgccgtgcga catcccattt agtggaatct ataacatgtg
     6121 ccagcgccca ggtttcatta ttcacattgg gcgttgctgc tgcggtaacc cctgacggca
     6181 actccgattt taatagcggg cgatgcagca tcttgccact taccgtggcg gtatccgcca
     6241 tatgacccca gaaatacgcc gtggcgacat taggactggt cggcacggtg aaaatcgtgt
     6301 ccagcgtcgc cgttattgtg gcgttatccg tcagcgtcac cgtcaacggc gttttatatc
     6361 ctggcgtatt atcctgacca acggtaaaac ttgctgtacc gtctgctcca gtaactccat
     6421 cgattgtatt cccgctatca gccaacgtga ctgccgcacc ggatgagggt tgtacagtca
     6481 aatcgtccat tgtatccaca tcgccataca acgtcgcccc gctattacgc ggcgtagcgt
     6541 caccacgttt gaggataaac gaaatattcg gcactggttt cccgacactg tctttcacgg
     6601 taacgacaac gggaacggcg tcgccttttt tcaccttcgc cgcctgcgta ctgctatcca
     6661 gtgccgtcga tgtcaacgtg acgctggaga cagtcgcacg cgggttaacc aggcaccctt
     6721 gatacgcggt attcaccgtc ccccacgctg aaccattatt cagaccgact acctgcggat
     6781 tactgcctcc cagccagtta tccagcgccc accatgtata ctgcgtagac caaccgattt
     6841 gtgtcgtcag cgtgttcccc ggatagcgag catagagcga ttctaaatca tcactcaatg
     6901 gccgacgtgc cgcttcacaa gggctgagat tgctgtcagc cctctgcgtt gcgttaaaca
     6961 cgggccagac ttcattgtta ctattgacct tgctaacccc tgatgtcgac ggcagctctt
     7021 tccacaattt aggtctttca aagaccacac cgttttctgc cgttaccgta tccggcatat
     7081 gaccccagta ggccgccatt gcggaatcag ggctggtgac aacggtgaag atcgcatcaa
     7141 ggacagtggt ggtttccgta tcacgtgcca ggatggcggt aaacggtgtt gccaggccga
     7201 cagtatcagg ctgttcaaca ttgaatatcg ccgtaccatc gctgccggta aaaccaagcc
     7261 attgcgtgcc gtgtgaggcg agagtgaatg ttgccgacac gggtgccagt tccgtcaata
     7321 ccatcacatt tgctgccgtg tttacaatgg ttcctgagcg gctcatcgca ttatcacgcc
     7381 tgagcgttat cgcttcgccc gccagcgggt tgccatcacg atctttgacc ataaccacaa
     7441 ttggcaatgg gttatctttt ttcactgacg cgctaggtcg gccgccattg ctgagggttc
     7501 tggcgtcatc cagtgcggat gacgtcaatg ttacgctaac gcctgccgga tgcggctgcg
     7561 ccagacagag catcagtgcg ttggttgcag aattcttcgc gatagctcct gttctcaggc
     7621 tgacagtctg ataagaccac gttgttgtac ccagtatggc catatcataa gcccaccagt
     7681 tacccgcatt catcggtaac ccaaggtcag tcattattgc gccgttcgga tgatcactat
     7741 acaaatccaa cagttcctta ctgcgcggtt gattctcgag tgggcaatct gtagatgtta
     7801 aattttgcaa gttgctcata tacgaccaaa cttcgccgtt agaactaatc gataagcctg
     7861 agctggtttc ggttttcagc aggggtcgtt tgaaggtgac gcccgccgaa ctggtaaagg
     7921 tgtccggcat atgcccccag aatttagcac tcggtgaatc tgggctggta atcaccgtgt
     7981 agataacatc cagcgtggcg actttcgttg tatcacgcgc cagcgtcacg gttaacggcg
     8041 tcgccaaccc ggtagtagag tactgcccaa cctcaaaggt ggctgtgcca tcactgcccg
     8101 tcgttgcgag agtttttgct ccacttgctg tcaacacgct atccctactc accggaacca
     8161 atgccgttac ggtaagatcg tctgcactcg tgttaacggt tgctttcgcg cgatccagcg
     8221 tcgtttcacg cgaaagggta aagtcggcat atggcatcgg gttcccggcg ctatcacgca
     8281 cggtcaccgt caacggaatc agctcacctt tcttcgctac agcgctcggt cgtccatcat
     8341 tgctgagcga ttttgctgca tcctgcgccg ttgaggtaag ctctattgtg actgcttccg
     8401 tatgaggctc taccaaacac atcactatcg ccgtatagct ggacgatgcc tgaatcaact
     8461 gaccatttcc cggaacaaac gcctggttac tccagctacc gcccgctacc gccatcatgt
     8521 cataatccca ccagttccct gcagcagtcg gcaagccgag caccgttgcc agttgaccat
     8581 tgggataatc gacatacagc ccctgcatat cagtcgtcag cggttgatac gcctcatcac
     8641 acccagcctt gctgacgtcc gctttctcag tcgcggtcag gagcgaccag acttcattat
     8701 tggccgtttt cgccgtaccg ccagagagtt cggctttgag gaaaggccgt ttgaaggtga
     8761 cgccttcgct gctggtaaag gtttccgcca tatggcccca attggtcgcc agcggcgtat
     8821 ccgggctggt ggtgacggta aagatcaccg acaaggtgtc cgacgacacg ccagtatcgt
     8881 ttaatagagc ataaaagtcg gttcttaacc cagtggaagc atcctgtcgg agcgtcatcg
     8941 tagtggttcc atctggccct gttacaccat acaggatggt cgttgcactg gtatccaccg
     9001 tcgcacccgc cgcagtcgtg actgacactt tctgcgatga cgtcacattc tgacgattga
     9061 tcgagttcgc gtgtttcagg gcaaacggcg tattgccgac taaattcccc tgcgcatctt
     9121 tagtcgaaat gcggatcgac acctcatccc ccttcttcgc tttgacggcg ctaagtgtgc
     9181 tgtcaatgct gacaaactgc ccggggtctg aggcttccag cgtaatctgg ctggcagtca
     9241 tcaacggcgt tgtacggcag gtttgtaacg ccagcacggt ggatacgatt tgcgaaactg
     9301 cgccagaatt tagttgcacc acattgtagt aacggctccc tgtttgtgta ttactcgggg
     9361 tattggtgag ataactcata tttaccggcc atccctgtac cgtattcatc gcattacctc
     9421 tattggcgtc atacaaagac ttcagaccat ccagcgttgg catataatct gtaccacacc
     9481 cattcatgct ggtgtcagcc gactgagtcg aggtgaatct tgcccaactt tcaccgttct
     9541 caaacgtgct atatcgtgca tagttgttgg taagctcgcc aaacagcaac ggtcgcttat
     9601 aaaccgaacc atcggaggcc gtcaccgttt cggccatatg tccccaaaaa ttcgcttttg
     9661 gcgaatccgg gctggtcacg acggtaaaga ccaccggtag catcgccttg acgctgctgg
     9721 acgtatccag ttttgccgtg agatcggttc ttaacccggt actgttatcc tgttttaggg
     9781 taaatgtcgt tgacccgtcg gcaccggtca cgccgtaaat ggtttcactc gttgactgga
     9841 aattatttcg cgtatttccc cacgcatccg tcaccgtcag cgcgcccaca ctcatcgtcg
     9901 ccgatgaatt agcccgactt acactgttgg ctcgcgtcag gataaatgcc gtattgccca
     9961 caaggttgcc ttgcgcatct ttggtgatga cgcgcaccac ggattcttcg ctttttttcg
    10021 cttttaccac gttataggtg gtatccagcg tcaccggatc tgccgcttcc agcaggattt
    10081 ggctcaccgc ggctatcggc gcggtctggc aggtcagcag ctccagcgtc gttgatgaca
    10141 ctgccgcact cgtgccattg ctgagatcta ccgccttgta gttacgctgc tccatgctgc
    10201 cgttgtcgga agtgttggat aaatagcgcg ttgccaccgg ccagccctgc acggttttca
    10261 tggtgttgcc cgcattatta gcaaacagtg acgtcagacc ggcctgagtc ggaatatagt
    10321 tggttccaca gccgctgtac gtcgtggaaa aacttgcata attaatgtta aacaacgccc
    10381 agttttcatt gtcttcagac gttgatgttc gcccagtctg ataggccagt tccttcaaca
    10441 acagcgggcg cttaaatacc gtaccgtccg cagcagtcac cgtttccggc atatgtcccc
    10501 acatttttgc caggcggcta tccgggctgg tcaccaccgt aaagattgtg tcgatgctgg
    10561 attttttcgt cgcatcagag taaagcattg ccgtcagcgc cgttttggtg ccgtgagtat
    10621 ccgggcgggt aatattgaga attttgctgc cgtcattgtc ggtcatggcg gtatacaccg
    10681 ttgcagtgtc gttcaacgtg gtttcatctg ccagcccgcc attgactacc accggcgcaa
    10741 ccagcgcatc gccgctgccc gccacatgtt tttcgccgct acgggtatag ccatcaccgc
    10801 gattgagggt aaatggcata tccgctaacg cgttgccctg tgcgtctttt accgtcactt
    10861 taacctgtag cgtctcgcct tttttcagcg ttgccgcgct attcccggta ttccattgcg
    10921 ctttatcaac cacctccagc gtaatgctgc tggcaggttt gttcgccgtc ttcaggcaac
    10981 tgacgtaaac cggcgtatcg ccgccaatag tttgtgcgcc atcgttcagc gctatcgtga
    11041 aaaagtgtgg agttttatct gccggtgagt tgctccagta aggctgacgc tgtgttggcc
    11101 aaccgtgcgc cgtttgcacc gcgttaccac tgttggcgtt atacagcgca cttagctggg
    11161 aacgacgtgg caacatattg gtgtcgcagc cacctttact ggtactggac gtattggcct
    11221 ggctcacgcg cgcccagatt tcgttatttt cggtcagggt tccgtccggg ctggcgactt
    11281 ccgacgccag tttaggccgg gtaaaggttg atgaatcaac ggtgatcgtt tcatccatat
    11341 ggccccacat ctgcgcgccg gtaacgtccg ggctggttgg ggtagtaaaa ataacgctgt
    11401 agttaacggt attcggcagg taggaattta ccggcgttac gctcagcacc gtttttagcc
    11461 cgacgccctg tggttgctta atctcgaccg tcgccacgcc ccgggcatcg gttattcccg
    11521 cataaacctt cgatgactgt gatgtgccgt attgcgtgtt atccatcgtg attgcaccac
    11581 tgctcgggtc atcaaacccg ctcacctgcc ccgctcggtt ataccccata cctttggtaa
    11641 tggtgaatgc agtaaagggt acagggatat tattgagcga gttaatcgta cgcacggtca
    11701 tcacaatggt gtcacccact ttggcttgcg cttgggtgcc aacagacaca tcatgatcgg
    11761 tgctgacttc aacattcgct accatctcat tacccgaaca ggagaggtaa ttcggcttaa
    11821 agccggaata gctgtcgacg ctcccgttcc ccagatccac cgatgaatgc gtggcttgct
    11881 ccaccgcgct taaatagccc tgttgtgcag tgggccagcc atattcagta ccaatcgcgt
    11941 taccgggatg ggctgaaaac aggctttcca gtgagctctg gcgcggtata tgaccgacgc
    12001 cgcattccgc ctgcatactg gtgttttgat cgtagagcgc ccagtcttcg ttattttccc
    12061 gcactgttcc caattcatgc tcggtttcat ccgccagcaa cgggcgttta tacaaactgc
    12121 cggactcaat aatgccgcgc atatggcccc acatccgcgc tttgtcgctg tccgggctgg
    12181 tgattacggt aaaaatcaca tctttggcat ctgtggcgtt aaaatcgctg cgcattcttg
    12241 ccgtgatatg ggttttcacc cccgcgccgc cgggctggct cagggtgagc gaagcctggc
    12301 cgttggcgtc ggtcatgcct tcataggtat gtgcgtcgac ctggcgaaaa ttactgccgc
    12361 cagcaatctg taccggatgg gcttcccagg cagtgtcggt ttgattctta cgattgacgc
    12421 catcatccag atgtagcgag aagtagtaat acgccagcgg ttgatcgttg tttttcgtgt
    12481 cggtgatggc cagacgcatc gtcgcatcct cgcccacctt cgctttcatc gcctgcgccg
    12541 tgtcatcata gttttctggc gtgaggacga ttttcggctc gacgtcaggc gcgggcttat
    12601 ccacgcagct cacgaggaac gtggtgctgt ccggcttctg cgtttcgcca cggttagaga
    12661 catctgccgc atggtgctgc ccggtaagcc ccgccagcga tgaccagtaa taatcgccct
    12721 gcatcggcca gccgagcaca gtctgtaccg tggagggaat caccgtcgcc agtgcgccga
    12781 agtggcgcat gcccggcagc acggtgcaat ggctatctgc gccgctccag gtaaaggtgg
    12841 accaggtttc attgtgatcg acgaccgtgg cattttcgtt actgacttcc gccgccagtt
    12901 ttggtctact gaacgtgtaa ccatgggctt ccaccgtctc cgccatatgg ccccacatcg
    12961 tggcctgcgc cacgtccggg ctggtgagca cggtaaagat caccgccgcc tcactggttt
    13021 gtgcaatgcc ggaaatgccg actaccagcg gcgttttaac accggggccg ttaggctggg
    13081 tcaccgtgat ggttgccgta ccgttagcat ccgatgtgcc acgatactcg gttaccgtcg
    13141 tcgttaactc ggtgctatcc agcaccaccg gcgcggtatt atttaccgcg ccctgacggt
    13201 tttgggcatc tttacgcttg ataataaatg gaatattacc cgcgacgtta ccctgacaat
    13261 ctttggtggt gacggtcagc gtgatagtgc cgccaacggt ggtttttgcc gcgccgagcg
    13321 tttcgttata accactggcg ctcaccgaaa ttaaatcgcc ctgcgcgggc gcgatattca
    13381 gcgtcacttt ctggtcagcc agcaccaccg tcccctcata gggataaccg gtcgaggtca
    13441 gcagataaag cagtggaatg gtctgctgcg catcaatctg tggccaggcc accagcgaac
    13501 gcccggccag accttgcgcg caaaagctgc ggttggcgat gggctgtgga ttgagcggcg
    13561 tatccggcgt ggcggcatcc gcccacacca gactgacggt cggcggcgtc tggttttccc
    13621 agcgcagcgc aggtggtgta gaaaactggt cgccttcact gtcctgagga ttagcaacta
    13681 tcaggttagc agcgtcatcg tcaacgctaa attcattcgg tttagcagaa aacgccacat
    13741 catgagatgc agcaggatca agctgaacgc ttccctgata aacgggtacg ctggcgctat
    13801 cggcaatagg aactgtaccg ttaaactcgc ctgtagaacc aggatatgtc tgccattcca
    13861 cctctgcccg tgcaaagtca gacagcataa aggctgccaa catgcccaga agcacaatga
    13921 tttttcctgg tttcagacat accgacatgt agaaaactcc agcatgataa acaaaacgtc
    13981 aacccgtttt gcttaacgcg tcgctaagca aaacaggcgg aaaacagcat tcccatacgg
    14041 gaatgctgtt gtgcgcatta gtggtgtttg taaatgatag acagcgtata gccctgaacg
    14101 ccgtcgccgt ttgccgggat tgcaaggccg gtcttaccat tacggtcact accgttaagg
    14161 ctggttcttg cttgttcgtt agtttgcgga ataacaatgt cattgttatc gaagctggcg
    14221 ttggcaatac cgcccgccgc cgcaagttgt ttactgttac cgttaaagac ccacgcaaag
    14281 tcgtagttag cggtaacgtc cacatccgta cccgcgtcgt atttaccgtt ctggttttca
    14341 tccgagtaca gtttggccac atacgttttg ttaagcgcaa tggctgtatt accaccgata
    14401 aggttggtac tggtgtcttt ctcgaagatg gcaactttca ggttctggtt aacaaccgga
    14461 ccaggatcga cattatccgt atcgctgccg ccaccgctgg ccgtagaaat atccagcaga
    14521 gtcagcgaag ttccctggaa gggattaccg gtctgagtga taggctggat ttcaacaccg
    14581 atatagcgtc cacgatcgcc atcggtgatt ttataactgg tttttcctgc cgccgcagtc
    14641 aactccgttt taccgccacc cgcattatca ctgtagctgt accatttgat ggtcgccgtc
    14701 gttttcgcat caatgcctgc ggaaccatca tcgatatccc cttcggtatc gccgatatcc
    14761 caacccagcg tgatggtatc ccctgagtaa aggcgtttat cagactcgct acctgcagga
    14821 cggatacctt tactatcaga tgtcactttg atgtgatccg catcttccga gttaatcgta
    14881 gcattccccc cttcgcgata gagccagggt atggtcccct cgaattcagc tgtcgttcca
    14941 ctggcgaccc aggcagcatc ttccgccata gcgactggta cggcacagta tccagccagc
    15001 atcagtgcca gcgcgacttt tgtcaggcca cgtttcatat gaggtttcat aaacactctc
    15061 cttggttaac attcagcggt ggcccgatca gccatggcgc tgttcctatg tcttgctgta
    15121 attgacctgc aaaccgtacc cttgcacacc atcggttacc tgggcactaa acttcgctgc
    15181 cgcttccttg ttcgttgccg ggatctcgat atcgacgtta gtggtattgg cttgcgcgcc
    15241 agtattgcca tttacgctat gccccgtgaa ttcccattgg tagtcatact gtttcatctc
    15301 ttcgtcagtg agcttttcag atagctggaa cacaccgtct ttgttagcgt cgcgccacaa
    15361 cacaaagcga tatcggctat tgaggggcaa cggttcttta ttgccgataa gattcacgga
    15421 tttagccgca ccaacgcgat agataaaggc attcagcccg ccgatgtcag cccccaggaa
    15481 cgttacgtca acatagttac tgtcatcata gggggcagct ttcgccttcc aacggaaggt
    15541 gccaggcaac gtactggaga gcgtaacggt ccccttttct ttatcttgtt tggcgataaa
    15601 gacacaggga gtctcggtag tgcaggggtt attcgtcagc gtcacttttt tatcgttggc
    15661 atcaaacagc tgcgggttga gattatcctg cggcaagaca ttaccgttgc ggtcagtgtt
    15721 gtcgccaaag ctatcagtaa tatcgagatc catcaccaca ccatctacac catcagcctg
    15781 tgcactggct tcgtggctgt ggttgttctc ttccggcacg ttcgcgatgt tattaacaat
    15841 cagctcaatt ttacggtcct gctgaacggt gatttccacc acgcctgagt tctgtcggtt
    15901 gcccttttca tctttcagcg ccaggcgaac ccgccaggta ttgagttttc cttgttcggc
    15961 tttgtcagag ctgtactgcc agacgggcag caccaggttc cagtggttat tgttgccctc
    16021 tgtcagggta ttctctttga taaccggcat caccagcgtg cgggcggcat tgtttttcgc
    16081 ctgccatgac gcagcgacaa ttttgtagtg gtggttgaca agcgcgttaa tggtccattt
    16141 acatttttcc agcccgatag cttcttctgg cgtatcttta atcacgcact cgggatgttc
    16201 attcatcgca tcagctttca gccacaaagt gacatcgagg ggatctttct ctttatattc
    16261 cagaacaata aagttgttac gttcaacaaa gtcgtgacgg ctgcccatga gtgagtgagc
    16321 cgcgctcact ttgtcaggat caagctggtc tttgagcggg acgccaaact ggtagttaag
    16381 cgttgcgttg accgtggtat cggtgttatc accggttcct gctttgaagt ccgttcctac
    16441 agcaatgagc ggaacaggct ggtatttcac gcctaacgtg acagcgaagg gatctttctc
    16501 tagattgtcg gtaccaaata gcgcaacttc atccccgtaa taatgttcga aaacgatttt
    16561 accgccaagc tgcggataag cgggaagcca ggcttcggca cgaatatccc aaccgctcgc
    16621 agggcgttct tcataaaagt cgaaatcttc agagtctttc cagtcagaaa gcggatgata
    16681 atagttacca gaaaatttta aataatcagt ccacgcttca gcgcctatac ctaaacggcg
    16741 atgtccacgg gtaaaatcat aatcataaaa tatatttcca cccaataacc atttatcaaa
    16801 attatgtctt acaccaagcc ctatatttcc gatcgtacga tcttcttttc gttgcgcaga
    16861 aaattggctg aaataaacag tggtttgatt atcgtaccag ggaacaaaat aatcgatgga
    16921 actgccatcc agatcgccgc cctgaccaat ggagaggttc gaacggacct taccgtaggg
    16981 agagagtgtg tcttcaatat aagattgtgc ggttgaggtg atttgacttt gcagatagct
    17041 tctggcctga gattccagcg cctcaggggt caagttttca aacccttgcg tggcggagtt
    17101 gatgacgtaa tcggctccgc gctctttcag cgatttaccc ttgttggttt gttcatcctg
    17161 ttgtgctgcc tggctaccta aatcaggtaa cgattcgttt tgcttaacat ttatctctgg
    17221 tgccgcgaaa gcagcaccag agataaatgt tagcataata cagcgtttcc atcgcagcat
    17281 gtgcttt
//
LOCUS       DACADA010000097        17287 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.099, whole genome shotgun sequence.
ACCESSION   DACADA010000097 DACADA010000000
VERSION     DACADA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.099"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            5..598
                     /locus_tag="ISB17_003128"
     CDS             5..598
                     /locus_tag="ISB17_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP1981027.1"
                     /translation="MDDLAKQQELQKSKIEELEKQQEQYKNKAELFEKQQNNITNSTR
                     ILASIVKTVKEKQDEFVFTEFNPLQTQYFILNNGSVGLAGRVLAIDAVENGSVIRISL
                     VNLLSVPVSNIGFHATWGNEKPTDAKALAKWQQLLFNTTLNSTLQLMPGQWQDINLTL
                     KGVSPNNLKYLKLSINMANLQFDTVQPAETRQRKNKK"
     gene            642..4985
                     /locus_tag="ISB17_003129"
     CDS             642..4985
                     /locus_tag="ISB17_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1981028.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            5403..7058
                     /gene="lldP"
                     /locus_tag="ISB17_003130"
     CDS             5403..7058
                     /gene="lldP"
                     /locus_tag="ISB17_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAP1981029.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7058..7834
                     /gene="lldR"
                     /locus_tag="ISB17_003131"
     CDS             7058..7834
                     /gene="lldR"
                     /locus_tag="ISB17_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAP1981030.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            7831..9021
                     /gene="lldD"
                     /locus_tag="ISB17_003132"
     CDS             7831..9021
                     /gene="lldD"
                     /locus_tag="ISB17_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAP1981031.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9069..9542
                     /gene="trmL"
                     /locus_tag="ISB17_003133"
     CDS             9069..9542
                     /gene="trmL"
                     /locus_tag="ISB17_003133"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP1981032.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(9595..10416)
                     /gene="cysE"
                     /locus_tag="ISB17_003134"
     CDS             complement(9595..10416)
                     /gene="cysE"
                     /locus_tag="ISB17_003134"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP1981033.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10496..11515)
                     /gene="gpsA"
                     /locus_tag="ISB17_003135"
     CDS             complement(10496..11515)
                     /gene="gpsA"
                     /locus_tag="ISB17_003135"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP1981034.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11515..11982)
                     /gene="secB"
                     /locus_tag="ISB17_003136"
     CDS             complement(11515..11982)
                     /gene="secB"
                     /locus_tag="ISB17_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAP1981035.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12046..12297)
                     /gene="grxC"
                     /locus_tag="ISB17_003137"
     CDS             complement(12046..12297)
                     /gene="grxC"
                     /locus_tag="ISB17_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAP1981036.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12438..12869)
                     /locus_tag="ISB17_003138"
     CDS             complement(12438..12869)
                     /locus_tag="ISB17_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP1981037.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13113..14657
                     /gene="gpmM"
                     /locus_tag="ISB17_003139"
                     /gene_synonym="pgmI"
     CDS             13113..14657
                     /gene="gpmM"
                     /locus_tag="ISB17_003139"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAP1981038.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14667..15950
                     /gene="envC"
                     /locus_tag="ISB17_003140"
     CDS             14667..15950
                     /gene="envC"
                     /locus_tag="ISB17_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAP1981039.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15954..16913
                     /locus_tag="ISB17_003141"
     CDS             15954..16913
                     /locus_tag="ISB17_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAP1981040.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16900..>17287)
                     /gene="waaH"
                     /locus_tag="ISB17_003142"
     CDS             complement(16900..>17287)
                     /gene="waaH"
                     /locus_tag="ISB17_003142"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAP1981041.1"
                     /translation="LHNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIY
                     PEFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALL
                     WSFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         4262 a         4492 c         4557 g         3976 t
ORIGIN      
        1 tctgatcgat gatcttgcca aacagcaaga gttgcaaaaa agtaaaatag aggagcttga
       61 gaaacaacaa gaacaatata aaaataaagc agaactgttt gaaaaacaac aaaataatat
      121 cacaaacagc accagaatac ttgccagcat tgtaaagaca gtcaaagaaa aacaggacga
      181 atttgtcttc accgaattta acccgctaca aacccaatac ttcattttaa ataacggctc
      241 ggtcggtttg gcaggccggg tgctggctat tgatgcggtt gaaaatggca gtgtcattcg
      301 tatttcgctg gttaacttat taagcgttcc cgtatcaaat attggctttc atgcgacatg
      361 ggggaatgag aaacccaccg atgccaaagc tctggcgaaa tggcaacaat tactattcaa
      421 caccaccctg aattccactc tgcaattaat gcctggtcaa tggcaagaca ttaatctgac
      481 attaaaaggc gtctcgccta ataacctgaa gtatctgaag ttgtccatca atatggctaa
      541 tcttcagttc gacactgttc aacctgctga aacccggcag cggaaaaaca aaaaataaat
      601 gcagcttctg cgcttatatc aaggaataga gagcatcaat aatgaacaaa atatttaaag
      661 ttatctggaa tccggcgaca ggcagttaca acgtcgccag cgaaacggcg aagagccgtg
      721 gcaaaaagag cgggcgcagt aagctgttaa tttctgcctt ggttgcaggt gggctgttat
      781 cgtcatttgg ggcactggct gataactacg aggggcagcc agtggattat ggcaatggct
      841 cagcaggtga cggctgggtt gctatcggta aaaattcaaa agcaaatacc tttgtcaatg
      901 gctctggtgc gagcactgcg ctaggttatg acgcgatagc tgaaggtgag tacagctcag
      961 ctataggctc acgaactcat gcgattggtg gtgcatcaat ggcctttggg gttagtgcac
     1021 aagcaatagg tgacagaagt gtcgcgctgg gtgcatcttc agtagcaact ggcggtcgtt
     1081 cgatggctct tggtcgttac gcaaagacga atggttttac atctcttgct attggggaca
     1141 ccgcccttgc cgatggtgaa aaaactattg cgttaggaaa tacggctcaa gcttacgaaa
     1201 ttatgagcat cgccctcggt gatcatgcta atgcctcaaa agaatattca atggcgctgg
     1261 gagcaagtag cgttgcatct gcggctaacg ccattgccgt aggccgcgac agtgctgcta
     1321 acggcacaga cagccttgcc ttaggcagac tatctctcgc cagcgcagca aacgcgattg
     1381 ctatgggtgc agagagcgaa gctgctgaaa atgcgacggc gattggcttt aacgcggatg
     1441 cgatcgggaa gtccagcctg gcgctgggag acaacgccag cgctggtgaa accaactcca
     1501 tcgctatagg tcaaggcagc gaggcaagca aacttgattc aattgcgctg ggaagtaaca
     1561 gcaggtcagc gggtgagaac gccattgcac tgggcaacaa cagtaatgcg gggggcaaaa
     1621 acagccttgc tttcggtttt aacaccaccg caaatggtga taacgcagtc gccattggtg
     1681 caaattcttc cgcaggtgct gacaataccg tgtctgtagg tagctctagt ttaaagcgca
     1741 aaattgtcaa tatgggtaat ggtgacatca ataatgtcag ctccgatgcc atcaatggat
     1801 cacaacttta tgccatcagt aagtctgtat ccgacaggct tggaggatat catgatgatc
     1861 ctgataatgt tataaactct gacgggacgc ttaaagctcc cacatattat ctacaaagtg
     1921 gccaatacaa taacgtggga gaggctctcc agagcatcga caataacacg ttgcactggg
     1981 acagcaaaag taataaatac agtgccagcc atactgtctt taatgcaaat ggctcagtta
     2041 aaagcacctc ggcgaaaaac atcattaccg acgtcgctga tggcacgatt tcagccacca
     2101 gttccgacgc cgttaacggt tcgcagttgt ataatctgaa acaagatgcc ttgctatggg
     2161 atggcactgc gtttagcgcc aaacatggca ccagcaatac taacagcaaa atcaccaacg
     2221 ttgcggatgg tgctgtttca gcctccagta aagatgccgt taacggttcg cagttgtatg
     2281 atctgaaaca agatgccctg ctatgggatg gcactgcgtt tagcgccaaa catggcacca
     2341 gcaatactga cagcaaaatc accaacgtac tggcaggcac agtatcttcc accagcaccg
     2401 atgccattaa tggtacacaa cttcaaagca ctgcaagcac cattacgtcc tatttgggcg
     2461 gcggcgcggc aatgagtaat gacggcgtat ttaccggtcc tcaatataat attggtggta
     2521 agaattacac caatgttggc gcagctctta ccgctctgga caatgacgcc ctgttgtggg
     2581 atgcaaccgc taattcaaat actggcgcat tcagtgctgc acacggaaca ctcaaaaccg
     2641 ccagcaagat taccaacgtt gcaaatggtg acatattagc caacagttat gatgctatta
     2701 atggctcaca actttatagt ttagctgatt catttacgtc ataccttggc ggtggtgctg
     2761 atatcaacaa tgcgggtgtt ctcatagccc cgacctatac tatcggttcg aaaacgtaca
     2821 acaacgtagg tgatgccctt aacgcgatta actcatcatt cagcacctca atcagtgatg
     2881 ccctgctgtg ggattctaca gcgaacaaat tcagcgccaa acacggtggt accaatagca
     2941 ttattactaa cgttgcagac ggtacaattt cctctaccag cagcgatgct gtaaatggct
     3001 cgcaactcta tgacacgagc aagtacattg ctgatgccct gggtggtgac gcagaagtta
     3061 acgctgacgg caccatgact gcaccgactt actctatcgc taatgaagat taccacagcg
     3121 taggtgaagc cctggatgcg ctcgatgata acgcgctgct gtgggatgaa gacgcaggtg
     3181 cctacaacgc cagccatgat ggcaatgcca gcaaaattac caacgttgcg gcaggtgatc
     3241 tctccacaac cagtaccgat gctgttaacg gttcccagtt aaacgcaacc aatattctgg
     3301 ttacgcaaaa tagccaaatg attaaccagc ttgccggtaa caccagcgaa acctacatcg
     3361 aggaaaacgg tgcgggtatt aactatgtac gtaccaacga caacggctta gcgttcaacg
     3421 atgccagcgc ctcaggtatt ggtgctacgg ctgtgggtta taactctgtt gccgaaggtg
     3481 atagcagcgt ggccattggt caaggtagca gcagctccgt tgataccggc attgccttgg
     3541 gtagcagctc cgtttccagt cgcgtgattg ttaaaggttc tcgtaacact agtgtaacgg
     3601 aaaatggcgt agttatcggt tatgacacca ctgacggtga gttgctcggt gcattgtcaa
     3661 ttggtgatga cggtaaatac cgtcaaatca tcaacgtcgc cgatggttcc gaagcccatg
     3721 acgccgttac ggttcgccag ctgcaaaatg ccattggtgc agtcgctacc acgccgacta
     3781 aatacttcca cgctaactca acggctgaag actcactggc agtcggtgaa gactcgctgg
     3841 caatgggcgc gaaaaccatc gttaatggta atgcgggtat tggtatcggc ctgaacacgc
     3901 tggttctggc tgatgcgatc aacggtattg ctatcggttc taacgcacgc gcaaatcatg
     3961 ccgacagcat tgcaatgggt aatggttctc agactacccg tggtgcgcag accaactaca
     4021 ctgcctacaa catggacgcg ccgcagaact ccgtgggtga gttctctgtc ggcagtgaag
     4081 acggtcaacg tcagatcacc aacgtcgcag cggggtcggc ggataccgat gcggtcaacg
     4141 tgggtcaatt gaaagtaacg gacgcgcagg tttcccagaa tacccagagc attactaacc
     4201 tgaacactca ggtcactaat ctggatactc gcgtgaccaa tatcgaaaac ggcattggcg
     4261 atatcgtaac caccggtagc actaagtact tcaagaccaa caccgatggc gcagatgcca
     4321 acgcgcaggg taaagacagt gttgcgattg gttctggttc cattgctgcc gctgacaaca
     4381 gcgtcgcgct gggcacgggt tccgtagcag acgaagaaaa tacgatctct gtgggttctt
     4441 ctaccaacca gcgccgtatc accaacgttg ctgccggtgt taatgccacc gatgcggtta
     4501 acgtttcgca actgaagtct tctgaagcag gcggcgttcg ctacgacacc aaagctgatg
     4561 gctctatcga ctacagcaac atcactctcg gtggtggtaa cggcggtacg actcgcatca
     4621 gcaacgtctc cgctggcgtc aacaacaacg acgcagtgaa ctatgcgcaa ctgaagcaaa
     4681 gcgtgcagga aacgaagcaa tacaccgatc agcgcatggt tgagatggat aacaaactgt
     4741 ccaaaaccga aagcaagttg agcggtggta tcgcttctgc aatggcgatg accggtctgc
     4801 cgcaggctta cacgccgggt gccagcatgg cctctattgg tggcggtact tacaacggtg
     4861 aatcggctgt tgctttaggt gtgtcgatgg tgagcgccaa tggtcgttgg gtctacaaat
     4921 tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt
     4981 ggtaatgatc cattaacgaa tgggtaaacg ccacattgcc tgatgcgcta cgcttatcag
     5041 gcctacgggg tgattcagca cattcctgta ggtcggataa ggcgttcacg ccgcatccgg
     5101 caagataagg cactccgggt caacaatcta agggcaattc tctgatgagg attgcccttt
     5161 tctttaccag acatctccca ccacaagaat tggccctacc aattcttcgc ttatctgacc
     5221 tctggttcac aattccccaa ttaaaactca catcaatgtt gccaacacgt aacatttagt
     5281 taaccattca ttgtcattac ccctacacaa cacaattggc agtgccactt ttacacaacg
     5341 tgtgacaaag agatgggcaa cagactcatt acacggcgtg tgtggactgc aggagacctg
     5401 caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagcctga
     5461 tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat
     5521 acgtcgccgc ctcatggacg gtggcaatcg cccttgccgt ggctttgctg ttctataaaa
     5581 tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctacggg ttgtggccca
     5641 tcgcgtggat cattattgca gcggtgttcg tctataagat ctcggtgaaa accgggcagt
     5701 ttgacatcat ccgctcgtct attctttcga taacccctga ccagcgcctg caaatgctga
     5761 tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag
     5821 caattaccgc cgcattgctg gtcggcctgg gctttaaacc gctgtacgcc gccgggctgt
     5881 gcctgattgt taacaccgcg ccagtggcat ttggtgcgat gggtattccg attctggtcg
     5941 ccgggcaggt aacaggcatc gacagctttg agattggtca gatggtgggg cgtcagctgc
     6001 cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta
     6061 tcaaagaaac ctggcctgcg gtcgtggttg cgggcgggtc gtttgccatc gctcaatacc
     6121 tcagctctaa ctttattggg ccggaactgc cagacattat ctcttcgctg gtatcgctgc
     6181 tctgtctgac actgttcctc aaacgctggc aacccgtgcg cgtattccgc ttcggtgact
     6241 taggggcgtc acaggttgat atgacgctgg ctcacacagg ttacacggca ggtcaggtgc
     6301 tgcgcgcctg gacaccgttc ctgttcctga ccgccaccgt aacactgtgg agtatcccgc
     6361 cgtttaaagc cctgttcact tcaggcggtg cgctgtatga gtgggtgatc aacattccgg
     6421 tgccgtacct cgataaactg gttgcccgta tgccgccagt ggtcagcgaa gcgacagcgt
     6481 atgccgccgt gtttaagttc gactggttct ctgccaccgg taccgccatt ctgtttgctg
     6541 ccctgctctc gattgtctgg ctgaagatga aaccatctga cgctatcagc accttcggca
     6601 gcacgctgaa agaactggct ctgcctatct actccatcgg catggtgctg gcgttcgcct
     6661 ttatctcgaa ctattccggt ttgtcatcaa cactggcgct ggcactggcg cacaccggtc
     6721 atgcattcac cttcttctcg ccgttcctcg gctggcttgg ggtattcctg accgggtcgg
     6781 atacgtcatc taatgctttg tttgccgctt tgcaggctac cgccgcacaa caaattggcg
     6841 tttctgacct gttgctggtt gctgccaaca ccaccggtgg tgtcaccggc aaaatgatct
     6901 ctccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt
     6961 tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca
     7021 cgcttcaggc ctatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct
     7081 gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg
     7141 catgaagttg cccgctgaac gtcaactggc gatgcaactc ggcgtgtcga gaaattcact
     7201 gcgcgaggcg ctggcgaaac tggtaagcga aggtgtgctg ctcagtcgac gtggcggcgg
     7261 gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctgaa
     7321 aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat
     7381 tgaagccagc accgcatggc atgcagcaat gcgcgccaca cctggcgaaa aagaaaagat
     7441 tcagctttgc tttgaagcaa cgctaagtga agacccggat ctcgcctcac aagcggacgt
     7501 tcgttttcat ctggcgattg ccgaagcttc acataacatt gtgttgctgc aaaccatgcg
     7561 cggtttcttc gatgtcctgc aatcctctgt gaagcatagc cgccagcgga tgtacctggt
     7621 acccccggtt ttttctcagc tgaccgaaca acatcaggcg gtcattgacg ccatttttgt
     7681 cggtgatgct gacggggcgc gtaaagcaat gatggcgcac cttagctttg ttcacaccac
     7741 catgaaacga ttcgatgaag atcaggctcg ccatgcacga attacccgcc tgcctggtga
     7801 ccataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc
     7861 gccgcagcgc aacgcattct gccgccgttc ctgtttcact atatggatgg gggggcatat
     7921 tctgaataca ccctacgccg caacgtggaa gatttgtcag aagtggcgct gcgccagcgt
     7981 attctgaaaa acatgtctga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg
     8041 atgccggtgg cgctaggtcc ggtaggtttg tgtggcatgt atgcgcgacg cggcgaagtt
     8101 caggctgcca aagccgcgga tgcgcatggc attccgttta ctctctcgac ggtttccgtt
     8161 tgcccgattg aagaagtcgc tccagctatc aaacgtccga tgtggttcca gctttatgtg
     8221 ctgcgcgatc gcggctttat gcgtaacgca ctggagcgag caaaagccgc gggttgttcg
     8281 acgctggttt tcaccgtgga tatgccgaca ccgggagcgc gttatcgtga tgcgcattct
     8341 gggatgagcg gcccgaacgc ggcaatgcgc cgctatttgc aggcggtgac gcatccgcaa
     8401 tgggcgtggg atgtgggcct gaacggtcgt ccgcatgatt taggtaatat ctcggcttac
     8461 ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg
     8521 tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc
     8581 aaagggatcc tcgacccaga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt
     8641 gtggtttcta accacggtgg ccgccagctg gacggtgtgc tctcttctgc ccgtgcactg
     8701 cctgctattg cagatgccgt gaaaggtgat atcgccattc tggcagatag cggaatacgt
     8761 aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact gctgggtcgt
     8821 gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg
     8881 atcgaaaaag agatgaaagt ggcaatgacg ctgactggcg cgaaatcgat tagcgaaatt
     8941 acgcaagatt cgctggtgca ggggctgggt aaagagttgc cggcggcatt ggctccgatg
     9001 gcgaaaggga atgcagctta agggttagac gaatatctgc tatcctgccc ccgcactaag
     9061 ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaaca
     9121 tcatccgtct ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg
     9181 cctgggacga taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta
     9241 cgcgtcatca tgactatcgc gcgtttctcg aagcagaaaa tccccagcgt ctgtttgccc
     9301 tcaccacgaa aggtacgcct gctcacagcg ccgtaagcta tcaggatggc gattatctga
     9361 tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac
     9421 aaaaaattcg tattccaatg gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt
     9481 cggtcgtggt gtatgaagcc tggcggcagt tggggtatcc gggagcggta ttgagagatt
     9541 agttactgta ggccggatag atgattacat cgcatccggc acgatcacag gacatcagat
     9601 cccatcacca tactcaaacg tatggttaat gccgttgaaa tgctgatcca tatccattga
     9661 cggtttatcg ctatccggct tgccgacaat gcgtgccgga acgccagcgg cagtagtgtg
     9721 cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gcccaacttc
     9781 aatattgccg agaattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg
     9841 acggtcgcca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc
     9901 gttttcaatc accgccgttt caccaacgac gatgcctgtc gcgtggtcga gcatgatacc
     9961 gcgaccaatt tttgctgccg ggtgaatatc aacctggaac gtcacagaaa cctggttttg
    10021 cagaaaaata gccagtgcgc gacgcccctg attccacaac cagtgaccga tgcgatacgc
    10081 ctgcaaagca tgaaaaccct tcaggtatag caacggggtt gagtatttat ccaccgccgg
    10141 gtcacgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcggc
    10201 ggcgtaggct tcttccacca cttcacggat agcaatagcc ggcataatgg gcgatgacag
    10261 cttgttcgcc agcatgtagc tcagtgcact gccaaggttt tcgtgcttga gtagcgtcgc
    10321 gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt cagctttaat
    10381 attgttccag acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa
    10441 tgaccgaccc gttctgcgcg ggccgggtca tagtggtaac aaaggttccc tgggtttagt
    10501 ggctgctgcg ctcgtccttg cgtgcacgac ctagtaaagt caatgctgcc tcgcgcgcgt
    10561 tttttccgca atataatact tgataaattt cctcggttat tggcatttca acgccgaagc
    10621 gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccact tgaccaattt
    10681 tctcctgcgc gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac
    10741 gcgactggtt gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg
    10801 tggcaggatc ggcacccagc gccgcgccaa gacgcgacat ctcagccagc ccacgagtga
    10861 tcagcgccgt acgcgcattc gcaccaaaac cgataccgtc ggacatcccc gcgccaatgg
    10921 caatgacgtt tttcaccgcg ccgccaagct gcacaccgat gaaatccggg ttgctgtaaa
    10981 cgcggaaact tttgccacag tgcagcaatt gctggaggtc atcggcaaag gtctggtccg
    11041 tcgaggccag cgaaatggcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc
    11101 cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca
    11161 gacgtccggt ttctgcttcc agccctttgg ttgcccacac cagacgcgca tcaggacgca
    11221 tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa
    11281 tattacggct ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa
    11341 aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt
    11401 cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggtg atggcaagag
    11461 cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc
    11521 atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc
    11581 atcgaagtta accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt
    11641 gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc
    11701 catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag
    11761 gaacgcggtt tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtaaac
    11821 gtcatctgcc agttgagtag atgccgtatc cagatcaagt ttaacttctg gttgccaatc
    11881 tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagaa atatccttgg tataaatacg
    11941 ttggatctgg aaagtcattt cggtgttgtt ttgttctgac atgtgtagaa aacccttaag
    12001 tgttgtcctt aaaatactgc gcaatgtaat gccaacgttc acacgttatt tcagcagggg
    12061 atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccaccaa tgtgctgtgc
    12121 gtcaataaaa atctgtggaa ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt
    12181 agcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc ttagcagtgc
    12241 ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat tggccataaa
    12301 ttagctcctg ttttttaccc tgaaatttca tgttgcaggg cggcagcaaa atggagtgtc
    12361 cccagaagct tatatcaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat
    12421 gaaagacgac aggtaaatta tttgccgcgc accaaaggca agttttcgcc agcccagcca
    12481 gcgacgcctt ctttcagtac gaatacttgt gcaaaaccag ctttcgtcag cgcgtttgca
    12541 ggctcctggc actgcatgcc agaaccatct accacgataa ccggtttgtc tttgtgcttc
    12601 tcaagctcac caacattgtt ggctttgatt tcgctcggca acaggttgat agaacctgcg
    12661 atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg
    12721 ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta
    12781 aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga
    12841 cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa
    12901 aaaaccagga aaggagtata cctgcgcagt gcggcaaata cagccagcgc gttaactgga
    12961 atgcaatttt gcggggcgct acgaaaaaaa agcgtaaatc tggcatctgc ttgccgcgag
    13021 cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc
    13081 aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac
    13141 tggtgattct ggatggctat ggctatcgcg aagaacagca ggataacgcc atttttagtg
    13201 ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt
    13261 ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta
    13321 acctgggtgc aggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaag
    13381 atcgcacttt ctttgctaat ccggtgttga ctggtgcggt agataaagcg aaaaacgcag
    13441 gcaaagcagt acacattatg ggtctgctct ccgcaggtgg tgtacacagc cacgaagatc
    13501 acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctacacg
    13561 cattccttga cggtcgcgac actccgccgc gcagtgctga atcctcgctg aaaaaattcg
    13621 aagagaaatt tgccgcgctg ggtaaaggcc gcgtagcgtc catcattggt cgttactacg
    13681 cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ttatgacctg ctgactctgg
    13741 cgcaaggtga gttccaggcc gataccgccg ttgccggttt gcaggctgct tatgctcgcg
    13801 acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg
    13861 caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc gcacgtgaaa
    13921 tcactcgtgc attcgtaaat gccgattttg acggcttcgc gcgtaagaaa gtggttaacg
    13981 tcgatttcgt gatgctgacc gaatacgctg ccgacatcaa aaccgcagtg gcttacccac
    14041 ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagctgc
    14101 gtatttccga aaccgaaaaa tatgcccacg ttactttctt cttcaacggt ggcgtagaag
    14161 agtcgttcaa aggcgaagat cgcattctga tcaactcacc gaaagtggct acctacgatc
    14221 tgcaaccgga aatgagctcc gcagagctga ccgaaaaact ggttgcggca atcaagagcg
    14281 gcaaatacga caccattatc tgtaactatc cgaacggcga catggtaggt cacaccgggg
    14341 tgatggaagc ggcggttaaa gcggttgaag cgctggatca ctgcgtggaa gaagtcgcga
    14401 aagcggttga gtccgtgggt ggacaactgc tgatcaccgc tgaccacggt aacgctgaac
    14461 agatgcgcga tccggcaacg ggtcaggcac acacagcaca caccaacctg ccagttccgc
    14521 tgatttacgt tggtgataag aacgtgaaag cggttgaagg cggcaaactt tctgacatcg
    14581 cgccgaccat gttgtcgctg atgggtatgg aaatcccgca agagatgact ggtaagccgc
    14641 tgttcatcgt ggaataatcc ctccccatga ggggaaaggc gattaatacc atgacacggg
    14701 ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt ctgagcgctg
    14761 gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg tgaccaactc aaatccattc
    14821 aggctgacat cgccgcgaaa gagcgcgcgg tacgccaaaa gcaacaacaa cgcgcaagcc
    14881 tgctcgcaca attgaaaaag caggaagaag cgatctctga agccacccgt aaactgcgcg
    14941 aaacgcaaaa cacgcttaat caactgaata aacagattga tgagatgaac gcgtcgattg
    15001 ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct cgccgcgcaa ctggatgccg
    15061 cgtttcgtca gggtgaacat accggtattc agctgattct cagcggtgaa gaaagccagc
    15121 gtgggcagcg gttacaggct tatttcggct atctcaacca ggcgcgacaa gaaaccattg
    15181 ctcagttgaa acaaacgcgt gaagaagtcg ctatgcagcg tgccgaactg gaagagaaac
    15241 aaagcgagca acaaacgctt ttatatgagc agcgcgccca acaggcgaag ctgacccagg
    15301 cgttgagcga gcgtaaaaag acgctggcag ggctggagtc ttccatccag caaggtcagc
    15361 aacagttgag cgagctgcgc gccaacgaat cccgcctgcg taacagcatt gcccgtgcgg
    15421 aagccgcggc gaaagcgcgt gctgaacgtg aagcgcgcga agcccaggcg gttcgcgacc
    15481 gccagaaaga agcgacgcgc aaaggcacca cctacaagcc gaccgaaagc gaaaaatcgc
    15541 tgatgtcccg taccggtggt ctgggcgcac cgcgcggtca ggcattctgg ccggttcgcg
    15601 ggccaacgct gcatcgctat ggcgagcagc tacagggtga attacgctgg aaagggatgg
    15661 tgattggtgc ctctgaaggt actgaagtta aagcgattgc cgacggccgg gtgattctgg
    15721 ctgactggct gcaaggctac ggtctggtgg tggtggttga gcatggtaaa ggcgacatga
    15781 gtctttacgg ctataatcag agcgcactgg tgagcgttgg ttcgcaggtt cgcgcgggcc
    15841 agccaattgc actggtgggc agcagtggcg gtcagggtcg gccttcactc tatttcgaaa
    15901 ttcgccgcca gggtcaggcg gtcaatccac agccgtggtt gggaagataa gttttgtttc
    15961 catttcgtcg taacgttctt gcatttgccg ctctgttggc gctctcctcc cccgtaattg
    16021 ctggcaaact tgccatcgtc attgatgatt ttgggtatcg cccgcacaac gaaaaccagg
    16081 tgctggcgat gccttccgct atctccgtcg ctgtattacc cgattcaccg cacgccagag
    16141 agatggcgac caaagcacat aacagcggtc acgaagtgtt gattcatcta ccgatggcac
    16201 cactgagtaa acagccgctg gagaaaaata cgctacgccc ggagatgagc agcgacgaaa
    16261 ttgagcgcat tattcgtagt gcggtcaata acgtgcccta tgccgtgggg atcaacaacc
    16321 acatgggcag caagatgacc tctaacctgt ttggtatgca gaaagtgatg caggcgctgg
    16381 agcgttacaa tctttacttt ctcgacagcg taaccatcgg taatacccag gcgatgcgcg
    16441 ccgcgcaagg cactggcgtg aaggtgatca aacggaaggt gttcctcgac gattcgcaga
    16501 acgaagcgga catccgtgtg caattcaatc gcgcaattga cctagcgcgt cgcaacggtt
    16561 caaccattgc cattgggcat cctcaccctt caacggtacg cgtgctacaa cagctggttt
    16621 ataacctgcc gccagacatt acgctggtga aagccagcag cttgctgaat gaaccgcagg
    16681 ttgatacgtc tacaccgccg aaaaacactg tgcccgacac accgcgtaat ccgttccgtg
    16741 gcgtgaagct gtgcaaaccg aagaagccgc tggaacctgt ttatgctaat cgcttctttg
    16801 aagtattaag cgaaagcatc agccagagca cgctgatcgt ttacttccag catcagtggc
    16861 aaggctgggg caaacagccc gaagcggcaa aacttaacgc tagcgcaaat taaaggcgct
    16921 acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat tgccataagc ggaaagacca
    16981 cagtaacgcc tggtaaccca ccttcacgct gcggacattg gtaatcaggc gcttatacat
    17041 accggaagta aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg
    17101 caccgcatgg caaacacgca atgcttcgta agttatttgc tgatgaaatt ccggataaat
    17161 cgtaattttg tcagcataat ttcgatttaa tttctccagc aggcgggtaa tcttaatata
    17221 gtgacgttga taattaaggt ttttattccc ttgtctatgc aaccgactca ctgacgtatt
    17281 atgcaga
//
LOCUS       DACADA010000098        17037 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.100, whole genome shotgun sequence.
ACCESSION   DACADA010000098 DACADA010000000
VERSION     DACADA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.100"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(8..1462)
                     /gene="xylB"
                     /locus_tag="ISB17_003143"
     CDS             complement(8..1462)
                     /gene="xylB"
                     /locus_tag="ISB17_003143"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAP1981042.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1533..2855)
                     /gene="xylA"
                     /locus_tag="ISB17_003144"
     CDS             complement(1533..2855)
                     /gene="xylA"
                     /locus_tag="ISB17_003144"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAP1981043.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3221..4213
                     /gene="xylF"
                     /locus_tag="ISB17_003145"
     CDS             3221..4213
                     /gene="xylF"
                     /locus_tag="ISB17_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1981044.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4291..5832
                     /gene="xylG"
                     /locus_tag="ISB17_003146"
     CDS             4291..5832
                     /gene="xylG"
                     /locus_tag="ISB17_003146"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAP1981045.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5810..6991
                     /gene="xylH"
                     /locus_tag="ISB17_003147"
     CDS             5810..6991
                     /gene="xylH"
                     /locus_tag="ISB17_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAP1981046.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7069..8247
                     /locus_tag="ISB17_003148"
     CDS             7069..8247
                     /locus_tag="ISB17_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAP1981047.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8355..9179)
                     /locus_tag="ISB17_003149"
     CDS             complement(8355..9179)
                     /locus_tag="ISB17_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAP1981048.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9499..11529
                     /gene="malS"
                     /locus_tag="ISB17_003150"
     CDS             9499..11529
                     /gene="malS"
                     /locus_tag="ISB17_003150"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP1981049.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11707..12960
                     /gene="avtA"
                     /locus_tag="ISB17_003151"
     CDS             11707..12960
                     /gene="avtA"
                     /locus_tag="ISB17_003151"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAP1981050.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(12999..13472)
                     /locus_tag="ISB17_003152"
     CDS             complement(12999..13472)
                     /locus_tag="ISB17_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1981051.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13574..14422)
                     /gene="yiaJ"
                     /locus_tag="ISB17_003153"
     CDS             complement(13574..14422)
                     /gene="yiaJ"
                     /locus_tag="ISB17_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAP1981052.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14623..15621
                     /gene="yiaK"
                     /locus_tag="ISB17_003154"
     CDS             14623..15621
                     /gene="yiaK"
                     /locus_tag="ISB17_003154"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAP1981053.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMCGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15633..16097
                     /locus_tag="ISB17_003155"
     CDS             15633..16097
                     /locus_tag="ISB17_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1981054.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16120..>17037
                     /locus_tag="ISB17_003156"
     CDS             16120..>17037
                     /locus_tag="ISB17_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HAP1981055.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT         4356 a         4308 c         4184 g         4189 t
ORIGIN      
        1 ataacgttta cgccattaat ggcagaagtt gctgatagag gcgacggaac gtttctcgtc
       61 gtggctgata agcggcataa cgctgcgcat ctggtagatg cgactgttct aacggtagtt
      121 gcggcaacaa ctcaatgagc gatttctctg gattcgccgc aatctgcgcc agccttgctg
      181 cgcccagtgc tggccccaca tcgccacccg tgcggtaatc cagttgctga ccgctaatgt
      241 ccgccagcat ttgacgccag tactcactac gcgccccgcc accaatcaac gtaacacttt
      301 gcggtttaat gccgcaggca tgcacgacat ccatgccatc tgccagcgca taacccacgc
      361 cttccagcac tgctcgcgcc agttcattgg ggccatgttg atgagtcaaa ccaaagaaaa
      421 cccctttcgc ctggggatta ttgtgtggtg tacgctcgcc agaaagataa ggcagaaacc
      481 aaactggctc ggcactttca tcagcctgtc gagctgcagt gattaaagca gggacattgc
      541 tcaggccggt taatttcgcg gcccaatcca gacacgaagc cgcactcagc attacagaca
      601 ttaaatgcca acgttgcggt aacgcatggc aaaagctatg tacggcgctt tctggcttgc
      661 ttaagaaccc ttcgctgaca gcaaaataga cccccgacgt ccccagcgat aacattgcct
      721 gattagcatc aaccattccc acaccaaccg caccagctgc attgtcgcca ccgcctgcga
      781 caactggcac cgccgccata ccccacgctt tcgcaacttc aggtagcaaa gcaccagtaa
      841 tttcgctgcc ttcgtataat gcgggcatct ggtcacgaga taagtggcaa gcctgcagca
      901 tgtcgtcact ccagtcacgc tttgcgacat ccagccacat ggtgccagct gcgtcagaca
      961 tatcgctggc aaactccccc gtcatacgca gacgcaagta atctttcggt aataatactt
     1021 tatcgatttg acggaatatc tccggctcat gccgctgaac ccatagcaat ttaggcgcag
     1081 taaatccggg catcatcagg ttgccggtaa tcacccgcga ttgcggaact cgcgcttcca
     1141 gcaaagtgca ctcttgcgca cagcgcccgt cgttccacaa aatggcaggg cgtaacaccc
     1201 gttgctgagc atccagtaag gttgcaccat gcatctgtcc ggcaatacct aatgctttaa
     1261 cgtcttgcag agaatgctga acgcccagag ctttcattgc gcgatcagtt gcctgccacc
     1321 actgttccgg gtcttgttcc gaccagagtg gatgcgggcg cgaaacggtc agcttttccg
     1381 tttgcgcagc aaccacctca ccctgctcgt tgagcaaaat aacttttacg cccgaggtgc
     1441 caagatctat cccgatatac atatcgatcg ttccttaaaa aatgcccggt atcgctaccg
     1501 ataaccgggc caacggactg cacagttaac cgttatttgt cgaacagata atgatttacc
     1561 agattttcca gttgctcctg gcgaccactc tgatgcaccg gagacaaatt atgttcctga
     1621 gcatatttgg ctaaatctgc cagtgacatt tggcctttca ggatttgctg gcctaattca
     1681 ctattccagc cggaataacg ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg
     1741 cgcgctgcaa ttttcagcgc caacgccatc gtatccatcg cgccgatatg accgtaaaac
     1801 agatcatatt tatcagtact ttgacgacgt actttggcat cgaagttcag accaccggtg
     1861 gtgaaaccgc ctgctttgag aatttcatac atcaccagcg cattctcttc cacactgttc
     1921 gggaactggt cggtgtccca gcccagttgc gcatcgccac ggttggcgtc gacagaaccg
     1981 aacaggccaa gcgcaatggc ggtggctatt tcatgatgga aagagtgacc tgccagcgtc
     2041 gcgtggttag cttcaatgtt cagtttaatc tctttttcca gaccaaactg tttcaggaag
     2101 ccatagaccg tcgcagcatc gtaatcatat tgatgtttgg tcggttcttg cggtttcggt
     2161 tcgataagca acgtgccctg gaaaccgatt ttatgtttat gctcaaccac catctgcata
     2221 aagcggccca gctgttcacg ctcctggcgc aagtcggtat ttaacagcgt ttcgtaacct
     2281 tcacgaccac cccacaggac atagttttca ccgcccaatt tatgggttgc ttccatcgct
     2341 gtaacaactt gcgttgccgc ccaactgaag acttcaggat ctgggttcgt cgccgcaccc
     2401 gcgccatagc gagggtttgt aaagcagtta gcagttcccc acagcagctt cacgccgctc
     2461 tcttcttgct tgcctgccag gacatcaacc atttgcgcaa aattattgat gtactctttt
     2521 aacgacgcgc cctcagggga aacatccaca tcgtggaagc aataaaatgg cacatgtaac
     2581 ttgtggaaaa actcaaatgc gacatctgct ttacgcttcg ccaacgccag tgcctcacca
     2641 ggctgctgcc acggacgatt aaacgccccc acaccaaaca tatccgcccc gttccagcag
     2701 aaggtgtgcc agtagcaggc ggcaaaacgc aagtgctctt ccatacgctt gcccaacacc
     2761 agttcgtcgg gattgtagtg acggaatgct aacgggtttg aggattttga gccttcataa
     2821 cgaacgcgat cgagctggtc aaaataggct tgcatattga actccataat caggtaatgc
     2881 cgcgggtgat ggatgatgtc gtaatattgg gcactccctt tcagttgctc aattatgtta
     2941 tttcacactg ctattgagat aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat
     3001 gatgaaactg ggtaattcct cgaagagaaa aatgcaataa gtacaattgc gcaacaaaag
     3061 taagatctcg gtcataaatc aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct
     3121 gtaattgttt tcccctgttt agttgctaaa aattggttac gtttatcgcg gtgattgtta
     3181 cttattaaag ctgtcctcta actacagaag gccctacacc atgaaaataa agaacattct
     3241 actcaccctt tgcacctcac ttctgcttac caacgttgcg gcacacgcca aagaagtcaa
     3301 aataggtatg gcgattgatg atctccgtct tgaacgctgg caaaaagatc gagatatttt
     3361 tgtgaaaaaa gcagaatctc tcggcgcgaa agtatttgta cagtctgcaa atggcaatga
     3421 agaaacacaa atgtcgcaga ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat
     3481 tattccttat aacggtcagg tattaagtaa cgttgtaaaa gaagccaaac aagaaggtat
     3541 taaagtatta gcttacgacc gtatgattaa cgatgcggat atcgattttt atatttcttt
     3601 cgataacgaa aaagtcggtg aactgcaggc aaaagccctg gtcgatattg ttccgcaagg
     3661 taattacttc ctgatgggcg gctcgccggt agataacaac gccaagctgt tccgcgccgg
     3721 gcaaatgaaa gtattaaaac cttatgttga ttccggaaaa attaaagtcg ttggtgacca
     3781 atgggttgat ggctggttac cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc
     3841 caataataac aaaattgatg ctgtagttgc ctcaaacgat gccaccgcag gaggggcaat
     3901 tcaggcatta agcgcgcaag gtttatcagg gaaagtagca atttccggcc aggatgcgga
     3961 tcttgcaggt attaaacgta ttgctgccgg tacgcaaact atgacggtgt ataaacctat
     4021 tacattgttg gcaaatactg ccgcagaaat tgccgttgag ttgggcaatg gtcaggagcc
     4081 aaaagcggat accacactga ataatggcct gaaagatgtt ccctcccgcc tgctgacacc
     4141 gatcgatgtg aataaaaaca acatcaaaga tacggtagtt aaagacggat tccacaaaga
     4201 gagcgagctg taagcgttac gccccggcgc ggagcggggg cgtgatttct ctccatgccg
     4261 cgttaattaa ttggcttagg tggagtcgtt atgccttatc tacttgaaat gaaaaatatt
     4321 accaaaacct tcggcagtgt gaaggcgatt gataacgtca gcttgcggtt gaatgctggc
     4381 gaaatcgtct cactctgtgg ggaaaatggg tcaggtaaat caacgctgat gaaagtgctg
     4441 tgtggtattt atccccatgg ttcctacgaa ggcgaaatta tttttgcggg agaagagatt
     4501 caggcgagtc acatccgcga taccgaacgc aaaggtatcg ccattattca ccaggaattg
     4561 gcgctggtga aagaactgac cgtgctggaa aatatcttcc tgggtaacga aataacccat
     4621 aatggcatta tggattatga cctgatgacg ctacgctgtc agaagctgct cgcacaggtc
     4681 agtttatcca tttcacctga tacccgcgtt ggcgatttag gacttgggca acaacaactg
     4741 gttgaaattg ccaaggcact taataaacag gtgcgtttgt taattctcga tgagccgaca
     4801 gcctcattaa ctgagcagga aacgtcggtt ttactggata ttattcgcga tctacaacag
     4861 cacggtatcg cctgtattta tatttcgcac aaactcaacg aagtcaaagc gatttccgat
     4921 acgatttgcg ttattcgcga cggtcaacac attggtacgc gtgatgctgc tggaatgagt
     4981 gaagacgata ttatcaccat gatggtcggg cgagagttaa ccgcgcttta ccctaatgaa
     5041 ccacatacca ccggagatga aatattacgt attgaacatc tgacggcatg gcatccggtt
     5101 aatcgtcata ttaaacgagt taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg
     5161 ggtatcgccg gactcgttgg tgccggacgt accgagacta ttcagtgcct gtttggtgtg
     5221 tggcccggac aatgggaagg gaaaatttat attgatggca aacaggtaga tattcgtaac
     5281 tgtcagcaag ccatcgccca ggggattgcg atggtacccg aagacagaaa gcgcgacggc
     5341 atcgttccgg taatggcggt tggtaaaaat attaccctcg ccgcactcaa aaaatttacc
     5401 ggtagtatta gccagcttga tgacgcggca gagcaaaaat gtattctgga atcaatccag
     5461 caactcaaag ttaaaacgtc gtcccccgac cttgctattg gacgtttgag cggcggcaat
     5521 caacaaaaag cgatcctcgc tcgctgtctg ttacttaacc cgcgcattct cattcttgat
     5581 gaacccacca ggggtatcga tattggcgcg aaatacgaga tctacaaatt aattaaccaa
     5641 ctcgtccagc agggtattgc cgttattgtc atctcttccg aattacctga agtgctcggc
     5701 cttagcgatc gtgtactggt gatgcatgaa gggaaactaa aagccaacct gataaatcat
     5761 aacctgactc aggagcaggt gatggaagcc gcattgagga gcgaacatca tgtcgaaaag
     5821 caatccgtct gaagtgaaat tagccgtacc gacatccggt ggcttttccg ggctgaaatc
     5881 actgaatttg caggtcttcg tgatgattgc agctatcatc gcaatcatgc tgttctttac
     5941 ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc tccaacctgt tacgccaaac
     6001 cgcaattacc ggcatcctcg cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga
     6061 cctttccgtc ggctcaatga tggggctgtt aggtggcgtc gcggcgattt gtgacgtctg
     6121 gctaggctgg cctttgccac ttaccatcat tgtgacgttg gttctgggac tgcttctcgg
     6181 agcctggaac ggatggtggg tcgcgtaccg caaagtccct tcatttattg tcaccctcgc
     6241 gggcatgttg gcatttcgtg gcatactcat tggcatcacc aacggcacga ctgtttcccc
     6301 caccagcgcc gcgatgtcac aaattgggca aagctatcta cctgccagca ccggcttcat
     6361 cattggcgcg cttggcttaa tggcttttgt tggctggcaa tggcgcggaa gaatgcgccg
     6421 tcaggctttg ggtttgcagt ctccggcctc taccgcagta gtcggtcgcc aggctttaac
     6481 cgctatcatc gtattaggcg caatctggct gttgaatgat taccgtggtg ttcccactcc
     6541 tgttctgctg ctaacgttac tgttattggg cggcatgttt atggcaacgc ggacggcatt
     6601 tggacggcgc atttatgcca tcggcggcaa tctggaagcg gcacgtctct ccgggattaa
     6661 cgttgaacgc accaaacttg ccgtgttcgc tattaacgga ttaatggtag ccatcgccgg
     6721 attaatcctc agttctcgac tcggcgctgg ttcaccttct gcgggaaata tcgccgaact
     6781 ggacgcaatt gcagcatgtg tgattggcgg caccagtctg gcaggcggcg tcgggagcgt
     6841 ggccggagcg gtaatggggg cattcatcat ggcttcgctg gataacggca tgagtatgat
     6901 ggatgtaccg accttctggc agtatatcgt taaaggtgcg attctgttgc tggcagtatg
     6961 gatggactcc gcaaccaaac gccgttcttg attttgataa aaattttctc aaagccggta
     7021 acgtattacc ggttttgagt ttttgcatga ttcagcagga aaagaaccat gtttactaaa
     7081 cgtcaccgca tcacattact gttcaatgcc aataaagcct atgaccggca ggtagtagaa
     7141 ggcgtagggg aatatttaca ggcgtcacaa tcggaatggg atattttcat tgaagaagat
     7201 ttccgcgccc gcattgataa aatcaaggac tggttaggag atggcgtcat tgccgacttc
     7261 gacgacaaac aaatcgagca agcgctggct gatgtcgacg tccccattgt tggggttggt
     7321 ggctcgtatc accttgccga aagttaccca cccgttcatt acattgccac cgataactat
     7381 gcgctggttg aaagcgcatt tttgcattta aaagagaaag gcgttaaccg ctttgctttt
     7441 tatggtcttc cggaatcaag cggtaaacgt tgggccactg aacgcgaata tgcatttcgt
     7501 cagcttgtcg ctgaagaaaa gtatcgcgga gtggtttatc aggggttaga aaccgcgcca
     7561 gagaactggc aacacgcgca aaatcggctg gcagactggc tacaaacgct accaccgcaa
     7621 accgggatta ttgccgttac tgacgcccgg gcgcggcata ttctgcaagt atgtgaacat
     7681 ctacatattc ccgtaccgga aaaattatgc gtgattggca tcgataacga agaactgacc
     7741 cgctatctgt cgcgtgtcgc cctttcttcg gtcgctcagg gcgcgcggca aatgggctat
     7801 caggcggcaa aactgttgca tcgattatta gataaagaag aaatgccgct acagcggatt
     7861 ttggtcccac cagttcgcgt cattgaacgg cgctcaacag attaccgctc gctgaccgat
     7921 cccgccgtta ttcaggctat gcattacatt cgtaatcacg cctgtaaagg gattaaagtg
     7981 gatcaggtac tggatgcggt gggcatatca cgctccaatc ttgagaagcg ttttaaagaa
     8041 gaggtgggtg aaaccatcca tgccatgatt catgccgaaa agctggagaa agcgcgcagt
     8101 ctgctgattt caaccacctt gtcgatcaat gagatatcgc aaatgtgcgg ttatccatcg
     8161 ctgcaatatt tctactctgt ttttaaaaaa gcatatgaca cgacgccaaa agagtatcgc
     8221 gatgtaaata gcgaggtcat gttgtagcga tgatgagaat tgtcggcgtc gcttcaggta
     8281 actcagcaca accacctgat gggagcaaaa gccttatccg actgacaagt cggataaggc
     8341 tcgaggaaat gcaattacat atgcgccgcg attaaccgtt ggttatcctg gtacattgca
     8401 aacaggtagt tgttataact cttccctttg gtcgagtagc ccttcagctt gtgaatcatc
     8461 gctgtggcag tcacttcctg atccgcttta cgcagctgtg cacgcgattt acggaacgaa
     8521 gagtaagccg ggtgcgtatt gaggtttgta acataggcgc tcaccgattc tttaacagaa
     8581 ctaaactgtg agtacccttt cactttaccc ggcgcattgg tacaacgtcc tttcatgcat
     8641 ttcatgccga aaagattgtt gttgttgcgc gccagctttg acgttcccca accgctttct
     8701 gctgcagcca tcgtcgccac catactggta gggataatgt ctacgcgttc aagcaaggta
     8761 ttccacggga tttttcgcgt attaccagac cacttcacct tgtagcgttt ggcgatgtct
     8821 ttcagacgcg cacgctcagc aggtgaccat tggccctgat actgttttga aatcagccag
     8881 ttacgttccg cagtaatggc cgcattttgg ctggtaatgt aaggcattac agtccggaga
     8941 aacgcctttt tccttggtgt tccggaaggg tattttcgca aatcaggaag tgaactactc
     9001 tttgcactat tgcgagaata ctcttgttta ctgcttacct gtttattact agctttagtt
     9061 aagtgggact tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac
     9121 accagagtga gtaacataag aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc
     9181 aggtctcctg gtcggattta atcattccaa caccttatat tttttacaaa tttgagagtt
     9241 gaatctcaaa tcatatcaaa aatagctgtc aagagcaccc caaggaatag tccaaatctg
     9301 aaactatgtc acgagttaac gattcagatt ggcgctaaat cgcagaaaat gtggggttta
     9361 tcgcaaaatt cagccgtttt ttgcgcgcga tcgctcaccc ttgcttctca tcctgtggag
     9421 ttaccgctca gggatgagtt ttgtctggct tatcgctggc aaactgtctg aaatcgcagc
     9481 aataaggact catccgccat gaaactcgcc gcctgttttc tgacactcct tcctggcttc
     9541 gccgttgccg ccagctggac ttctccgggg tttcccgcct ttagcgaaca ggggacggga
     9601 acatttgtca gccacgcaca gttgcccaaa ggtacgcgtc cactaacgct aaattttgac
     9661 caacagtgct ggcagcctgc ggatgcgata aaactcaatc agatgctttc cctgcaacct
     9721 tgtagcaaca cgccgcctca atggcgattg ttcagggacg gcgaatatac gctgcaacta
     9781 gacacccgat ccggtacacc aacattgatg atttccatcc agaacgccgt cgaaccggta
     9841 gcaagcctgg tccgtgaatg cccgaaatgg gatggattac cgctcacact ggatgtcagc
     9901 gccacttttg cggagggagc cgcagtccgg gattattaca gccagcaaat tgcgatagtg
     9961 aagaacggtc aaataacgtt acaacccgct gccaccagca acggtttact cctgctggaa
    10021 cgggcagaaa ctgacacatc cgcccctttc gactggcata acgccacggt ttactttgtg
    10081 ctgacagatc gtttcgaaaa cggcgatccc agtaatgacc agagttacgg acgtcataaa
    10141 gacggtatgg cggaaattgg cacttttcac ggcggcgatt tacgcggcct gaccaacaaa
    10201 ctggattacc tccagcaact gggcgtcaat gctttatgga taagtgcccc atttgagcaa
    10261 attcacggct gggtcggcgg cggtacaaaa ggtgatttcc cgcattatgc ctatcatggt
    10321 tattacacac aggactggac gaatcttgat gccaatatgg gcagtgaagc cgatctacgg
    10381 acgctggttg atagcgcgca tcagcgcggt attcgtattc tctttgatgt cgtgatgaac
    10441 cacactggct atgccacgct ggcggatatg caggagtatc agtttggcgc gttatatctt
    10501 tctggtgacg aagtgaaaaa aacgctgggt gaacgctgga acgactggaa acctgccgcc
    10561 gggcaaacct ggcatagctt taacgattac attaatttca gcgacaaaac aggctgggat
    10621 aaatggtggg gaaaaaactg gatcagaacg gatatcggcg attacgacaa tcctggattc
    10681 gacgatctca ctatgtcgct ggcctttttg ccggatatca aaaccgaatc aactaccgct
    10741 tctggtctgc cagtgttcta taaaaacaaa acggataccc acgctaaaat catcgacggc
    10801 tttacccctc gcgattactt aacccactgg ttaagtcagt gggtccgcga ctatgggatt
    10861 gatggttttc gggtcgatac cgccaaacat gttgagttgc ccgcctggca gcaactgaaa
    10921 accgaagcca gcgctgcact tcacgaatgg aaaaaagcta accccgacaa agcattagat
    10981 gacaaacctt tctggatgac cggtgaagcc tggggccacg gcgtgatgca aagtgactac
    11041 tatcgccacg gcttcgatgc gatgatcaat ttcgattatc aggagcaggc ggcgaaagct
    11101 gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa tggcggagaa attgcaggat
    11161 ttcaacgtgt tgagctacct ctcgtcgcat gatacccgcc tgttccgtga agggggcgac
    11221 aaagcagcag agttattatt attagcacca ggcgcggtac aaatttttta tggcgatgaa
    11281 tcctcgcgtc cgttcggtcc taccggttct gatccgctgc aaggtacgcg ttcggatatg
    11341 aactggcagg atgttagcgg taaatctgcc gccagcgtcg cgcactggca gaaaatcagc
    11401 cagttccgcg cccgccatcc cgcaattggc gcgggcaaac aaacgacact ttcgctgaag
    11461 cagggctacg gctttgttcg tgagcatggc gacgataaag tgctggtcat ctgggcgggg
    11521 caacagtgac ttttccggct tcccgttcgt gagtacctcg ggaagccgcc aaccaggata
    11581 aaatgtcagc cctaatcagc attgcaggat aaagccccgc tcactcttca acagaccgat
    11641 ttgcacccca gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat
    11701 cccgctatga cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg
    11761 ctgatggaag atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt
    11821 aatccggcgc agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg
    11881 gaaagtggca aagcgactga tgcactgtgc aattacgacg gtccacaggg gaaaacggag
    11941 ctactcacac tgcttgccgg aatgctgcgc gagaagttgg gctgggatat cgaaccacag
    12001 aatattgcac taacaaacgg cagccagagc gcgtttttct acttatttaa tctgtttgcc
    12061 ggacgccgtg ccgatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt
    12121 ggctacgctg acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa
    12181 ctgctgccgg aaggccagtt taaataccac gtcgattttg agcatctaca tattggcgaa
    12241 gaaaccggga tgatttgcgt ctcccggccg acgaatccaa caggcaatgt gattactgac
    12301 gaagagttgc tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat
    12361 aacgcttatg gcgtcccgtt cccgggtatc atcttcagcg aagcgcgccc gctatggaat
    12421 ccgaatatcg tgctgtgcat gagtctttcc aagctggggc tacctggctc ccgctgcggc
    12481 attatcatcg ccaatgaaaa aatcatcacc gccatcacca atatgaacgg cattatcagc
    12541 ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg taacgatctg
    12601 ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc
    12661 gccatcattc gccgctattt accggaagat cgctgcctga ttcataaacc ggaaggagcc
    12721 attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct ctaccagcgc
    12781 ctgaaagcac gcggcgtgct gatggtgccg gggcacaact tcttcccggg actggataaa
    12841 ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc ggagaaaatt
    12901 gaggcgggtg tgaagattct ggcggaagag atagaaagag cctgggctga aagccattaa
    12961 tacgctattc cggcgttgag aaacgccgga aaaacgtttc aaacaggctg ctgccgtagc
    13021 cgatgccgct gcactttctc gacatccatg cactgcaacg cctttgtcgg gcaggcttca
    13081 acacaggccg gccccgtcgc ccgatgccag cacagatcgc acttaatcgc cctcgccttt
    13141 ttgcgcgaag aaacgacctc catcgcacca aacgggcaag ccagcataca gcttttacag
    13201 ccaatgcaac gtgattgttc aacgaaaata tgcccatgtt cgcggcttat cgcgtcaaca
    13261 gggcagacat tcgcgcacgg tgcatcttca cactgatgac aggctactgc cgtggtccag
    13321 ctgtggtctt taatgacacg aatacgggaa ataaactcgt ctggtgacaa cgcagcgcaa
    13381 tcctgattct cctgatgcga cactgcgcaa gccacttcac aggtacggca accgatacat
    13441 ttcgtcgcat ccgcaataat aaaccggttc atgatcactc ctttgcacta aaacagcaga
    13501 gtgccagaga gcgcgtccct actgctttga tccaacgaag gaaatgttaa agttttggca
    13561 cttgtccaaa gggttatgta atcgcgccct ggtcgtccct gacagtaaat cccagctcat
    13621 tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag atttttctct cccacctgtt
    13681 tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg cacccgccca tgaatatcaa
    13741 acaccggaac agcaatacag gagacgccga gttcgttttc ttctctgtcc atcgccgctc
    13801 cactttcacg aatgtgcgcc agttcgtcga acatcgcggg cagctcggta atggtattgc
    13861 gggttaacgg ctggatctca tgctgatggc tttcccagta tgacttcacg tagtccgggt
    13921 gaccaaacgc catgtagatc ttgcccattg cggaacagta gagcggcata tgctggccta
    13981 tataggcacg ggttcgcagc atcccggttg tgggttccag cttataaatc aaaatagcgt
    14041 gatcgtcttc gcggctggag aagttaatgg tttcaccagt ggcgatgttc agtgcctcaa
    14101 gatgcggagc ggcgatatga atgatattca gcgaagatag cgccttctgc ccgacggcaa
    14161 taaatttggt ggtcaggcga taactccctg cggcgggcgc ggtggtcaca tagccgcagg
    14221 actgtaatcc ctgcaataag cgatggacgg tactcttatt taaaccagcc agctccgaaa
    14281 gatgcgccaa cggacaaccg tttggatagt tgctcaaaat ctcaatcagc atcaacccac
    14341 gaaacagact ctggcttccg gctggacgct ctttttcctg cgccatctcg ttctcttttt
    14401 ttcccatcac ttctttcccc attttgtcgc gtcctgatgg tagcgcaaag tgtgccgtag
    14461 ttcacgatct cgacagataa tttataacca cttgattttt aagttttttg aaattcatca
    14521 atcagattgc tttgttaaaa agtgatcgat atatttgaaa tcaagtttcg tatattgaaa
    14581 ttttaagcca aaaaagcgat caaaaaaaca aggaagcctg ggatgaaagt gacatttgag
    14641 cagttaaaag cagcctttaa tcgggtctta atttcacgcg gcgttgacag cgaaacggct
    14701 gacgcctgtg cagagatgtt cgcccgcacc accgaatccg gcgtttattc tcacggcgtt
    14761 aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg atatcattcc tgatgcccaa
    14821 cccaaacgta taaccagcct cggcgcaatt gaacagtggg acgcccagcg ttcgatcggt
    14881 aacctgacag cgaaaaagat gatggatcgc gccattgaac tggctgccga tcacggtatt
    14941 ggtctggtgg cactacgtaa tgccaaccac tggatgtgcg gcggcagcta cggctggcag
    15001 gcggcggaaa aaggctacat tggcatttgc tggaccaact ccatcgccgt aatgccaccg
    15061 tggggcgcaa aagagtgtcg catcggcacc aacccgctga tcgtcgccat tccttccacg
    15121 ccgatcacca tggtcgatat gtcgatgtcg atgttctctt acggcatgtt agaagttaat
    15181 cgcctggctg gtcgtcagct cccggtagat ggtggctttg atgatgaggg caatttgacc
    15241 aaagaacctg gcgttatcga gaaaaatcgc cgcattttgc cgatgggcta ctggaaaggt
    15301 tcgggcatgt cgattgtgct ggatatgatc gctaccctcc tttctgacgg cgcatcggtt
    15361 gccgaagtca ccgaggacaa cagcgacgaa tacggcattt cacaaatttt tattgccatt
    15421 gaagtggaca agcttatcga cggtcccacc cgcgatgcca aactgcaacg cattatggat
    15481 tacgttacca gtgccgagcg tgctgacgaa aatcaggcca ttcgcttacc cggccacgaa
    15541 tttactaccc tgctggcaga aaaccgccgt aacggtatta ccgttgatga cagcgtgtgg
    15601 gcaaaaatcc aggcgttata aggagataag ccatgatttt tggacatatc gcgcagccta
    15661 atccgtgccg tttgcccgcc gccattgaaa aggcgctcga ttttctgcgc accaccgact
    15721 tcaacgccct ggatccgggc gttgtcgaaa tcgacggcaa gaatatttat gcgcaaatta
    15781 tcgatttaac cacacgcgaa gcggtggaaa accgtccgga agtgcatcgt cggtatatcg
    15841 atattcagtt tctggcgtgg ggtgaagaga aaattggcat tgccattgat acgggaaaca
    15901 ataaagtcag cgaatcacta ttagagcagc gcgatattat tttttatcac gatagtgaac
    15961 atgaatcatt tatagaaatg ataccgggta gttacgctat tttcttcccg caggatgtac
    16021 atcgcccggc atgtaataaa aatgccacta cggcaatacg caagatcgtg gttaaagtgg
    16081 ccctcgctgc tttataaaaa atgattactg gagcacataa tgaaaacgaa taatgccggt
    16141 tatattatcg gcgcgtatcc ctgtgcacct tcttttcacc aaagaagtga agaggaagag
    16201 aaggatttct ggcgacaatt atctgagact ccagacattt tgggtctgga acaaccttgc
    16261 ctggaaaatt ttcaccctct tggcgatcaa tggttactcc gtcatacgcc agagagctgg
    16321 aaatttgtcg tcaccgcagt gatggaaacc atgcgtcgcc gcagcgaaaa cagcgggttt
    16381 ggtctcgcct ccagtgatga agaacagcgc caggcctgcg tggcgtacta tcgccacctg
    16441 ttcaataaaa tcaacgcatt gcaggctaat aaagtgctcg cactggcgct ccaggctgca
    16501 ccgttagcaa cgaatcctaa tgttatgcaa gcgacggacg cttttgcccg ctcattgaaa
    16561 gaaatcgcca gttgggactg gccgtgcaag ctggtgctgg aacactgcga cgccatgaca
    16621 tcttcttccc cccgaaaagg ctttttgcct ttagaaaatg tgctggaagt tattaccgat
    16681 tatgacatca gcatctgtat caactgggcg cgctctgcca ttgaagggcg caatacaacg
    16741 ctgccgctga cccacaccca aatggcgaaa caagccggaa aactcggcgc gttgatgttc
    16801 tctggtacga cgctaaacgg ggcatacggc gaatggcagg atttacacgc accgtttgca
    16861 ccattctgcg cagaaagcct gatgaccaca gatcatgttc gcgagttatt taatgtagca
    16921 gaatcgtcaa cattgcattt tgctggcatc aagctactgg aaataaatgc gacggctgat
    16981 gtgcaccatc gcatcgaaat attacgtaac ggcattcact ctctgaatga atctcgt
//
LOCUS       DACADA010000099        16795 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.101, whole genome shotgun sequence.
ACCESSION   DACADA010000099 DACADA010000000
VERSION     DACADA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.101"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            46..888
                     /locus_tag="ISB17_003157"
     CDS             46..888
                     /locus_tag="ISB17_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadC family transcriptional regulator"
                     /protein_id="HAP1981056.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEALIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(947..1924)
                     /locus_tag="ISB17_003158"
     CDS             complement(947..1924)
                     /locus_tag="ISB17_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HAP1981057.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRELQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAVRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQILLQAVNGQARISSQTVRNLLG"
     gene            complement(1941..2870)
                     /gene="flgL"
                     /locus_tag="ISB17_003159"
     CDS             complement(1941..2870)
                     /gene="flgL"
                     /locus_tag="ISB17_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HAP1981058.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLVVRDDLLEAANGTN
                     TADSLASLGQDIESLTDSMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(2885..4261)
                     /gene="flgK"
                     /locus_tag="ISB17_003160"
     CDS             complement(2885..4261)
                     /gene="flgK"
                     /locus_tag="ISB17_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP1981059.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(4451..4750)
                     /locus_tag="ISB17_003161"
     CDS             complement(4451..4750)
                     /locus_tag="ISB17_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HAP1981060.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMLVKQLSPRHK"
     gene            complement(4750..5850)
                     /locus_tag="ISB17_003162"
     CDS             complement(4750..5850)
                     /locus_tag="ISB17_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP1981061.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMTATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(5865..6530)
                     /gene="flgH"
                     /locus_tag="ISB17_003163"
     CDS             complement(5865..6530)
                     /gene="flgH"
                     /locus_tag="ISB17_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP1981062.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(6613..7398)
                     /gene="flgG"
                     /locus_tag="ISB17_003164"
     CDS             complement(6613..7398)
                     /gene="flgG"
                     /locus_tag="ISB17_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP1981063.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(7477..8214)
                     /locus_tag="ISB17_003165"
     CDS             complement(7477..8214)
                     /locus_tag="ISB17_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAP1981064.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(8214..9416)
                     /gene="flaE"
                     /locus_tag="ISB17_003166"
     CDS             complement(8214..9416)
                     /gene="flaE"
                     /locus_tag="ISB17_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlaE"
                     /protein_id="HAP1981065.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(9688..10401)
                     /locus_tag="ISB17_003167"
     CDS             complement(9688..10401)
                     /locus_tag="ISB17_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HAP1981066.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(10401..10832)
                     /gene="flgC"
                     /locus_tag="ISB17_003168"
     CDS             complement(10401..10832)
                     /gene="flgC"
                     /locus_tag="ISB17_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP1981067.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(10835..11170)
                     /gene="flgB"
                     /locus_tag="ISB17_003169"
     CDS             complement(10835..11170)
                     /gene="flgB"
                     /locus_tag="ISB17_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838822.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP1981068.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            11252..11989
                     /gene="flgA"
                     /locus_tag="ISB17_003170"
     CDS             11252..11989
                     /gene="flgA"
                     /locus_tag="ISB17_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP1981069.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGETV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            12071..12349
                     /gene="flgM"
                     /locus_tag="ISB17_003171"
     CDS             12071..12349
                     /gene="flgM"
                     /locus_tag="ISB17_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAP1981070.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            12362..12790
                     /locus_tag="ISB17_003172"
     CDS             12362..12790
                     /locus_tag="ISB17_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAP1981071.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRAREDL
                     LAVNDEITQHYEQLHAHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            12918..13850
                     /locus_tag="ISB17_003173"
     CDS             12918..13850
                     /locus_tag="ISB17_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HAP1981072.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRQSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQAILSTLTLPDLTPELYLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQPARQLMTLLG
                     SCAFDWSILPESPRKTSLPLQMPLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRESENAVLIRQQIQAERSADPLLYAFSLLSC"
     gene            13897..16335
                     /locus_tag="ISB17_003174"
     CDS             13897..16335
                     /locus_tag="ISB17_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP1981073.1"
                     /translation="MQTLIKTLVTHRVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAVLTDIVSDPLLRNAYLGLHNATHYQAIHHQAIDQVVLINLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLHTDKWLACYPSLCYQRQGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSHKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWCAVQGQLAEIRHHYCDAFMDGK
                     AGERAAMVIMEALG"
BASE COUNT         3623 a         4755 c         4527 g         3890 t
ORIGIN      
        1 agcactatca ggaaacattt atcccttgct aaggtaaact caactatgag tattattatc
       61 aacaactggc ggatggaccc gtcgcttaat gccttaatac attgcgaaac gggtgaaaca
      121 cgtcgtctcg gcgaatacca ttttattttg ctggaaacat tagcaaaaaa tgccgatgtg
      181 gttttatcgc gatcttattt atgtgccgaa gtatggaaga accgcatagt cggcggtaac
      241 agcctgccga cggcgatcca tgcgttgcgc gttgctatcg atgatgatgg caaacaacag
      301 aacattatta aaacaattcc gaagaaaggg tacctgtgca ataaagagta tgtgtctttt
      361 ccggagtcat cgcctgcgga agcgctgatt attaccgacc agatacagaa aacaacgcca
      421 gaagagatat cttcgacaac accgcctgta cctgttagaa aaaaacataa aggactgatg
      481 gggctggcgt tggccgcggc tgttatattg gttggctcaa ccgtggggta ttcacacttg
      541 aaaagtacac ctgacgcccc gcaactggta aaagagtcaa ttaacagtcc aagaataaaa
      601 atatttcatc ttagttctgg aaaggaaaat aattccgtac cgttactgtc acaaatcctc
      661 gcaccaggaa aagacaaact cgataattta ttgtcagcgc ataatatgac aatgacaacc
      721 tattataaat atgttcgtaa ccgactggaa agcgatattg ttctgcgtaa ccagtgcaac
      781 ggcagctggc aattaacctt taatgtggaa ggttggcaga acagcgatat taatagcgcg
      841 atgtatcaga atttagagaa gttgttaaat actgtgcaga aatgctgaca agtgccaata
      901 tatgccggat gcgacgctaa acgcgtctta tccggccaac cgataattat cccaataaat
      961 tcctcaccgt ctggctggaa attcgcgcct gaccgttcac cgcctgcaat aaaatctgat
     1021 agttatgatt ggcgctatcc agcacgccgc cgaggttctc cgatgcctgc accgcgtttt
     1081 ccgtttgcgg gaagcgtgac atgccgtcga tcaactggcg ggctttttcc tgctgcaccg
     1141 ccatctgcgc gcgctgctga ttaatgcttt ctaacgtctg ggaaatcccc gccccaccct
     1201 gttgcaaact ttgcgctaaa cgcacggcct gcgacggttc ggcaaaggtt tttagtggca
     1261 taaacgccga cttatcacca tcggtgcgca cgcttaaggt gcgctcgatg tcaggccact
     1321 gtttttcggt ggtggagaaa ctgatgccgt ccgcttgctg atgtacctgc acgcccacgc
     1381 ggcgcagggc gttggtcagg cgcgtctggt actgcccggc gctgtcatcc tcagacagca
     1441 tcaccgccga aagctgggtc tgccgcccgt cagagacact gaacatgcgc gttcccggcg
     1501 tgggattgtg taccagattc gccagatccg gcgagtggaa ggtcacccgc gcttcgccct
     1561 gcaacactgg ttgtaactcg cggtccaccg cgccgccgga aagcgcggcg cgtttgtcca
     1621 gcatctgcat cagcgccgta ctctgcgcct gcccgccctt acgctggctg tgacgatagt
     1681 cgagcaactg ctgttccagt tgcccgagat agtgatccgc ctgctgcaac gtggtgagct
     1741 ggtcgttaag ctgtacgctg taacgctgcg ggcgagtggt aatcagcggc gaggccggat
     1801 attcactcgc cgtcgccggg agtttttgcc gcaccgcctg agtggacgca acggttgacg
     1861 acgataccgc agcgctatgt gcgccgccgg tggcgagtga tgaagatgta agtccgacct
     1921 gcatactgtt cctgaaagag ttagagaacg ctgaacaaat tcaactcact cactttcgag
     1981 taagtcttga gggtagcttc cattgccact tccagtccgg taaaggtgat cgacgccgga
     2041 ccgtaatcga gatcctgcaa tgaaccaatc aattcatcat tagatgtcga gatatcggtc
     2101 tgtgcatcgc tcaacatcga catggtgttt tgtgtctcgc cgagcgacgc aatcgaggcg
     2161 ttgaggtcgt ccgaggcggt gtccaccaca tcgacggcgt tctgaatatc gctctgcacc
     2221 tgcggatctg ccgggtcgac gtccgggttt tgcagttcct gcgacaagga attaagcgtg
     2281 ttaagcacgt ctaaattgct accgaaaaaa tcgctcgccg ccacgttggt gtccacttcc
     2341 acaccgttcg acacggtagt ctggcgatgg tcgctgttgc cctgatagct atagctgtcg
     2401 gtgacgccat cgccatcatc gtctaccgcg acaatcggcg gctgatcgtt gatggtgccg
     2461 ccaaacacgt agtgcccgtc ttcgtcctga taattgagcg ccgccaccat tgaatcggtg
     2521 agcgactcaa tatcctggcc gaggcttgcc agcgaatcgg cggtattggt gccgttcgcc
     2581 gcttcaagca ggtcgtcgcg taccaccagc aggctgttgt tgacgccatc gagaatcgat
     2641 tcctgctggc ttaagcccgc cgacgcggag tcgatattgc tctgatactg ttcaatcgcc
     2701 gactgctggc ggtttaactg ggtgatgcgc gtggcggcga ttgggtcatc cgacggctgc
     2761 aaaatttcct tgccggttgc catctgctcg accacatgcg ccagagagcc ggagagatta
     2821 ttaaaacttt gcgtcatcga gacgtaggtt tgttgggtgg ttactcgcat tttcgcgctc
     2881 ccgcttagct gcacatttcc agcaccgaat cgaaaatctc ggccccggcg gaaatgactt
     2941 tcagattagc ttcataaatt tgttgatagg tgatcaggtt caccgcttct tcgtccatgc
     3001 tgacgccgct gacgctactc tgctggtttt gcgctgcgga atagacattg gacgcggcat
     3061 cgacttccgt ctgattttgc tgactgtaaa tgccgatatt gctgatgatt gacgagcacg
     3121 cctgcccgac cgtcacactg ccgaggttgg ctatctccag cggttcggtg gagatattga
     3181 tcagcgcctg caggttgtcg ctgttgccgc tttcgtccgg tgaactggaa aacgccagct
     3241 catcggcagt gatatccggg ttaacggtca gtgggccatc ggcattgctg gcgtcataaa
     3301 taaacagtgg ctcgccgggg ttaccgttga gatcgtagcc ctgcgccagc tggttgttga
     3361 ccgcatcggc gaactgcgac gccatgctgt tgatggtgtc ggtcagcggc gtcagcacgt
     3421 cgttttgata gtcgaacaga gcgcctaacg aaccgccagt gtcggtggtc atggtcgagg
     3481 tggtgccagc gaaagtcagc gacatggtcg gtgtgccgtc ggcgttggtt tccagcgcga
     3541 tggtcgagct ttgctgcccg ctcaccagcg gctgaccgtt tttcagggtg acgttgtagt
     3601 tgccctggtc gtcaatattg acctgcacgt ccatcattcc gctcagttct tccaccatct
     3661 gatcgcgggc gtcgtacagc gcggaggcgt tatcgccgtt ggcttcggcc tgagcgattt
     3721 gctggttata gctggcgatg ccgctggtca gcgtattgat ttgcgatacc atcgcctgct
     3781 gctgactgat gatttccgtg ctttgcgagt cgatgtaatc cagcgtgttg tcgatgcgca
     3841 acgacagcgc cccggcttcg ctgatcacct gctcgcgcag ggcggaatca tcggggctgg
     3901 tggtcgcttc gttaagggca gcgaaaaagt tatcgaagcc gccgctcaga ctgctgttat
     3961 cgtcgctcag taccgcttcc agttgtgtga gatacccttg ttgggtgctg taatagccgt
     4021 aatcgctggc ggcataccat acctgattaa cctgatactg gttggagacg cggcgaatac
     4081 tgtcgacctg cacgccgtta ccagcgctgt tcggcgaacc accgctggca ccgatggtgc
     4141 tgatctccgc cacctggcgg gtgtagcccg tggtcatggc gttagcggtg ttttgcgccg
     4201 tgacgttcag ctccacctgc gccgttgatg cgccgctgta gccgatgtta atcatgtcca
     4261 tgttgggtcc ttaccggtaa atcgtgccgg ttaaaagcgg cgtgaagttg gggattctta
     4321 agttccgtaa accgggtgcc aaatgccgga tgcggcgtaa acgccttatc cggcctacat
     4381 actacatacg ggcgactcgt aggcctgata agacgcgaca gcgtcgcatc aggcaccgaa
     4441 cgccgaagcc tcatttatgc ctgggcgaca actgcttcac aagcatcgcc gcaatcccaa
     4501 tcccgtgctg ctgcgccagg gtgttacaca actcatcgtc ataaaacccc tgcatcatgc
     4561 gcaccgaatc gctgttaaac gggttatctt tcgaatcaaa caactcgttg gttttacgca
     4621 tctctttcag catgttgcgt aaaaaaatcg cctcaaactg ctccgcggcc tgcttaatat
     4681 cattggcctg aaccttcggc cccattaacg catcgctgcc gtcaatcccg ccgctggcgt
     4741 tcactttcat cagatcacct ccaggtcggc atccagcgcg cccgcctcat gcagcgcctg
     4801 taaaatcgac ataatgtcgt ccggcgttgc gcctaaactg ttcagcgcat tgaccagcgt
     4861 tttcaggctg ctcgactccg gcaaactcac cacgcccggg cgcgcatgat tcaccgcgat
     4921 attgctctgc ggcgttaccg ccgtgcgccc gcccgcaaaa gcatttggct ggctgacgtt
     4981 gctggtttcg ttaatcgaga cggtcaaact gccgtgcgac accgccgccg catgcagcgc
     5041 aacgccatcg cccatcacca ccgtgccggt acgcgagtta aacaccacgc gggcgcggat
     5101 tttctctgcc tgcacctgta catcatccag ttgcgacata aatgccaccc gcgcgccggg
     5161 gctggtgggc gcacgcacgc tgacgttggt agagctctgc gcagtagcaa tgccgccaaa
     5221 cgaggcgtta atcgccgccg caatattgtt ggcatcttta aacgacggac gtttcaggtt
     5281 gagggtgata gtgtcgccca tctgaaaatc gctggggatc tcgcgctcta ccgttgcgcc
     5341 gttaggaatt aacccggcgg ttggcgtatt aaccgttacg ctcgaaccgc tggccccggt
     5401 ggcagtcatc ccgcccacca ccacgctgcc ctgcgccagc gcataaactt cgccatccgc
     5461 gccgtgcaac tgggtcagca acaaagtgcc gccacgaata cttttggcat cgccaatcga
     5521 cgaaacagtg acatcgatgg tctgcccgcg cgagtacatc ggcggcagcg tggcgctcac
     5581 cgctaccgcg gcgacgtttt tcaccttcgg atcgatttta tttggcagtt gcacgccaaa
     5641 ctggcgcagc atattggtga tggtctggtt ggtgaatttg acctggtttt tatcaccagt
     5701 accgtcgagg cctaccacca ggctgtagcc gaccagttgg ttttcacgca cgccctgtac
     5761 gttgaccaac gactccagcg attgcgccag cgccacgccg ggcagcgtca gacttacagc
     5821 gatcgctgcg gcctttatcc agttttgcat tgcactctcc ggcattaaat cgggaacaga
     5881 ggatggttaa acagccgcgt caaccagcct gcggcgttgg cgtcgcttaa cgcgccgcgt
     5941 ccggcatagg agatgcgggc atcggcgatc cgctgcgagg aaaccgagtt gtcgttttgg
     6001 atgtcgtcgg cgcgcaccag gccgcttaag cggatgtact catcgccctg gttgagagtc
     6061 agccattttt cgccgcgaat ttccaggatg ccgttaggct gcaccgcatg gacggaaacg
     6121 gtgatctccc cgcgcaggct gttctgctgc tgggaagtgg cgctgccgtc aaagtcgcgg
     6181 ttggcgtcga tagagccgga aagtttatct ttggtgtgtc caaaaatcgt gggcgcgccg
     6241 atatcgacgg tgttgctttt gccgaagttg gttttcgcct gtttgctcga ctgggtggac
     6301 tcttcgagga tcaccgtcag gatatcgccc acgcggtaag cccgccggtc ggcggtgagc
     6361 gaccagttgt agccggtttc aaacacgccc ccggcacgcc cgtctgccgg aggcggcgct
     6421 tcggtggttg gtggcgcaaa gtatgcgtcg tcttttttga ccagaatggc ctgcgattca
     6481 cacccgctaa ggagtggaag cagcgccacc agccagagat aatttttcac gttctgcctg
     6541 tttaaataca gtgagttatg tgctcttgta ggtcggataa gacgcgtcag cgtcgcatcc
     6601 gacatttcgg ccttacagcg tctggctgat gtactgcaac atatcgtccg cagccgagac
     6661 cattttggcg ttcatctcgt acgcccgttg caccgtaatc atcgccacca tctcgttgac
     6721 gatatccacg ttcgatcctt ccagcgcgtt gtcctgcaag gtgccaagcc catcttcgcc
     6781 cggtacgcct tcggtcggct gaccgctggc ggcggtttcc agatagaggt tgtcaccctc
     6841 agcggaaagc ccggccgggt tggcgaagtt caccagcgtc agttgcccta actcagtcac
     6901 gtcgctgtcg ccgggtaaga tcgccgtcac cgtgccatcc tcaccaaaag caacattagt
     6961 cgcccccgcc ggaacgtcga tttccggctg caacggcaag ccctgcgagt tggtcagtac
     7021 gccgtcagcg ttcacctgca agttgccgtc gcgggtataa gcgatatcgc cgttggcctt
     7081 ttgcacctgc aaaaacccct gccccataat ggcgacattc atcgcgttgt cggtggtttc
     7141 gacgttgcct tcggtgaagg ttttctgagt gccgacaatg cgtacgccgc taccgaattg
     7201 cagcccggtc ggcataatgt tgttctgatc gagcatcgcg cccggcgctt cctgggtctg
     7261 gtaaaacaga tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc
     7321 gatgttgttg gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat
     7381 ccatagtgct gcgttcatgg tggtaaattc cgtgttattt tttggccgcg gttgccggat
     7441 gcggcgtgaa cgccttatcc ggcctacgat gtgcgattac gagccgcgca ataagcggtt
     7501 gcccgcgtcg ctgatatctt cggcggtttt catcatcttg atctgcgctt cgaactggcg
     7561 gttcatggcg atggacgaca tcatctcgct caccgccgag acgttgctgc cctcgagaaa
     7621 accgccgctg actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag
     7681 catcccttca ggatttttcg ccaggttcgc gaccgggata tccaccagct tcaggcggtc
     7741 gatatccatc gtcgcggtca cgtcaccgtc atcgggcgtc acggagagcg tgccgtcgct
     7801 gccaaacgaa gcaatggcgt tgggcggcag aatgatcggc ccgttatctc ccagcaccag
     7861 attgccgtcg atagtcagtt gcccctggtc atcctgctga atgttgccgt tgcgggtata
     7921 cacttcgttg ccgtttttat cctgcacagc aatgtaaccc gaaccctgaa tcgccacgtc
     7981 cagtgggcgt tcggttttct ccgcaacacc agtgctgtcg ttcacgccgc tggccccttc
     8041 ctgcgccata tagcgcgtgt cgaagccgcc gcctttcacc ttatcgttag tcgccattgc
     8101 catatcggcg cgaaaaccgt tggtgttgac gtttgccagg ttgttggcgc tgatctgctg
     8161 ctcgtacagg gtctgagaag ccccgctcag ggcggtataa atcaagcggt ccattacatt
     8221 gcctggaaca gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga
     8281 tagttacgct gggcggtcat caggttgact aactcgctgg tgatatcgac gttagagctt
     8341 tcgagcacgc ccgacgacag cgtgccgcag gtgccggaac cgggaacgcc aatcagcggg
     8401 gtgccggatt cgccggtttg tacccacgcg gtgccgctca ctgcctccag gccgttttca
     8461 ttcgggaacg tcgccagcac cacctgcccc tgcaacatgc gctcgccgtt gctgtaggta
     8521 gcgtaaactt tgccatcgtc atcaacctgc acaccgttct gagtggcaga ggcgtagccg
     8581 tcggcggcgt tggtggtgac cgaaaattca gagccgtatt gcgtacaggt cgagtaatcg
     8641 acggttaaat cgatgggtgc ggcggcgtcg gtctgaaact caatggtctg cggcgtggtt
     8701 ggcgaggtca gtttcccggt gttcgggtcg aaggttaacg tcgtcgccgg aacgccggtc
     8761 tgctgctgac cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttggtgaag
     8821 tactggcata ccgaatgttc gttgcctaat gagtcataca ccgtggtggt gtagctgtcg
     8881 gtgtacgagc tgctgttggt cgcgtcgaac ggtacggtag tgcggtcgat agccgcatcg
     8941 ctggcatcga agttggcggt aaaggtcagg ctgctgctgg cctgcgccgg aatattaccg
     9001 gttttgatct ggatatcggt gactgtaccg gtttgcaggg tgcccgtgtc atcgaccgga
     9061 tagccctgca aataagcgcc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac
     9121 gatccggcgc gggtataaga aatgttgccc gcgctgtcgc aggtaacaaa aaagccgtcg
     9181 tcgttaatcg ccagatccag cgcgttaccg gtggatacca gcgaaccgcc gcgcgaaata
     9241 ctttgcgcgg tgcctgccac gctgacgccc atcgcctgac ttccggcata catggctgaa
     9301 aactgggtgg tcatcgactt atagcctacc gttccggcgt tggcgatgtt gttactgatc
     9361 ccgtccagct gttcgttaac ggcattcagc cccgttgcag caatttcata actcattgtg
     9421 tcttcctgtt aattcagatt ttggcagcgt gcccgttgtt ttgccggatg cggcgtgaac
     9481 gccttatccg gcctacgaac gggtgcaaac ataaaaatat gcgcgaactc gtaggcctga
     9541 taagacgcgt taagcgtcgc atcaggcatc ttgcaccgaa cgccggatgc ggcgtgaacg
     9601 ccttatccgg cctacaaaac cattcggatt caatagtttc atgtaggcct gataagcgaa
     9661 gcgcatcagg caatttggca ccggacatca cgccacatca ctgtcggcat cgggtacgcc
     9721 aaactggcta atcatggtga acggcacttc gcctacaccg gcaacgttaa gcaccgggga
     9781 gctgccgtcg agcgggatgc gcacatcctc cacttcgccg gaaacttcaa tcggcacctc
     9841 ttcttccccg gtgttggtga ccaccgagac gtcgtaatcg cccggcggga taccgtaatc
     9901 cgccggatta atcgagaaat ccacctgacc tggcccgacg gacgaactgc cttccgggat
     9961 cagcgacacc gtatagtcat cgcctgcggc atcggtaaag tggagatcgg cggtggtaca
    10021 ggcgtcgtca agcgtggcgc gaccattaat ggtctggtcg ctcacctgca aggcggtggt
    10081 ctgcaccata atgtcgtcac ccaccaggtt gccgagcgcc atcacctgaa tgttgctcat
    10141 cagcaccgcg ttggtgttgg cctgcacgct catctcttcc atcatcgcca cctgcgccat
    10201 cgaagaaagc tggtcgatat attcggtggc gtcagtcggg tcggtcgggt cctggttttg
    10261 gatctcagcg acaaacagcg tcaggaaggt atcgacgggc gagctatcgc tgctactttg
    10321 cgtgtcgctg gccgccacgt tgttgctttc cgccgtggcg gtcggctgcg gtgattgcgc
    10381 attcagcgcc agggtattca tcaggcttct cccagtttca gcaaactttg ctgcatactt
    10441 ttcacgttgt tgagcacatc gacattggtt tcgaaatcgc gcgaagccga catcatgtcc
    10501 gccatctgtt ccaccacgtt gacgtcggga taccagacat ccccgttagc atcggcgagc
    10561 ggcgaatgtg gttcatagcg tttcacagct ccgccggttt gcagcacgtc ctgcacctgc
    10621 acactggcac cgtcaatggc atgacgatgt gtacccgcca gcaaactgtg gtgatacacc
    10681 gccgcaaaca ccgggctgcg cgctttatat gcctgcgcct cgctgctggc gggcgcattg
    10741 gcattcgcca ggttactggc aacggtattc agacgcagcg tttgcgccgt catcgccgaa
    10801 ccggaaatct gatagatatc agtaaatgac atgcttattt cccttcaatg gcctctctga
    10861 tgccgctgat ttgcagattc agaaaggtca gactgctttg ataatccata ctattttgtg
    10921 aaaaccgcgc ttgttcgctg ttcagttcca cggtgttgcc atcttccgac ggctgcatcg
    10981 gcacacggta ctggacatcc accgccgcgt tatttgcccg ttgcatctcc ctggcaaaat
    11041 caatatcttt agccttgaaa tttggcgtat cgacgttcgc cagattcgca gtcagtagct
    11101 cggtcctctc aagacgtaac ttcactgcct gcggatgtac tcccaatgcc tgctgaaaac
    11161 tgatccccat ttcctgcgtc actcctgctt caatccgcat acgctaagca atcgccgtgc
    11221 cattttttaa gtgactgaag agaaagggtt tatgagtttt caattttgca ggcggaagtt
    11281 ttatttcctc ctggcgctgg ccttaccggg ctacgccgcc gtccatcccg tgcagcacag
    11341 cgcgcgggaa caagtcaacg cccaggtact gaatgccgcc agccaaaaaa ttgaatcgct
    11401 ggcgcaacag cggcgctggc atgactatcg ctacaccttt aaggtctaca ttccctcgca
    11461 gattgcgacc gccgcgccct gtactaaaac acccggcgtt acgctgactt cgcctgggga
    11521 catcgccctc aaccggatga acttcaccgt cagttgccca caaagctggc agatgaacgt
    11581 agcggtgcgt ccggatgtac tggtgccagt agtaatggcg aagtcactgg ttgcccgcga
    11641 tacgccgctc accgcgaatg atgtggagtt gaagccttac aacgtcagtg cccagcgccg
    11701 ggaggtgttg atggaaccca atgacgcgat tggtttcagc agtaaacacg cgctgcaacc
    11761 gggcaggcca atcaccaaag aagaactgat atctccggtg ctggtcgagc gcgatcaacc
    11821 ggtgatgatt gtttaccaga gcgcggggat caccgcctca atgcccggcg tagcactgaa
    11881 aaatggtcgc aaaggggaaa cggtgaaggt gcgtaacgcc agcagccagc gggtcatcag
    11941 cgcgatggtg gcggaaagcg gcgtggtgac aacggtaagc gcggagtaat ttgcgcgggc
    12001 gattaaagtt ttgccgcctg ttaaccgctt taccggagta acccacccaa aataccgaga
    12061 cgaggttacg atgaaaatca ccccgactat gccaggcaat cacccgacag ccacaatggc
    12121 aggctccagt cagccgcgca aaagcacggc gaccaccgcc acgacgctga gcgccgatga
    12181 cattacccag gcgggattgc agtctgccca gcaaacgctg aacgaccagc aagagagcga
    12241 tatcgacagc gacaaagtgg cacaaatgca ggcgatgcta gcctccggca gcctgcaagt
    12301 ggacaccgag cagttagcca gcgacatgct gagttttttc cagaactaag aggccgatga
    12361 agtgagtatc cggttacaac aggtgaaggc gttgttgcag ggaattcgtg acgacgacgc
    12421 actgtacgat tccctgcgtg aacttctcca gcgtcagcgg atctgcatga ttcgccgcgc
    12481 cagagaggat ctgctggcgg taaacgacga gataacgcag cactatgagc agttgcatgc
    12541 gcatagccat cagcggcata gcctgctgaa aatgctcggt gttagcgtaa atcgtgacgg
    12601 tctggcgcag gtttttgcct ggcttccggc ggtacaaaaa gcggcagcgc aacagttgtg
    12661 gcaacgcctt gagcaaaaag cagaacgctg caaagcctat aacgataaaa acggcgagct
    12721 gttaattcgc cagtatgaat ttattcaggc ttttttaggc agcgaagcgg attttctcta
    12781 tcagggataa cgacaattcc ttcccccatc gcggaaacgg attttccgcc tgatccccaa
    12841 tcctttgttt ttgattaaaa aaattcccgg cacgcttatt gcacatcctg caaggtaata
    12901 aagactggaa actacctatg agttttattg aatgttacga accggaatat gtgcgcaatt
    12961 ttatggcacg ccaccccgat tcgcctttat acgtccgcaa ggtgtggaaa aacgagattc
    13021 gccagagcct gatgctgaca gagccagaaa gctgcgaagc ggtgctcaac gatgcctgcg
    13081 cctttgattt acagctcacc taccgcccgg tggaagaaaa tgccgcagaa ctctccgcgc
    13141 gcgatgcgat agtaaaccag gccattttga gcacccttac gctccccgat ttaaccccgg
    13201 agctttacct gtatgccgtc ggaattgtgc tctcccgcgc cagtaaaatg ccgggcagag
    13261 atggagatat tcttgcccgc ttgacgacgc tgccacaggc gctggccgat catgcacaaa
    13321 aaggcacgct acaggcacag ttcgcccagc tcccgcccgt gccacagccg gcgcgccagt
    13381 tgatgacgct gctcggcagt tgtgcatttg actggtcgat tttgccggaa agcccgcgca
    13441 aaacttccct gccgttgcaa atgcctttgc tgacgctaca tgacgccaac agcaaagccc
    13501 tgttacaaca gcagcttaag gtgcagtggc aaaccacctg gcaacagcat tttgccaccg
    13561 cgccgtggat gatgcgtaac tggctgattt accgcgttta ccatgaggtg attggacagg
    13621 cagacggcgc agattattgc ccgctggtgt gcgattttta tcttatccgc acgttaatca
    13681 gtttatggac gctggatggt tctccgctgc gacaggagga tattttcgcc ctcttcgcca
    13741 tgtttgaacg ctggcgagag agtgaaaatg ccgtgctgat tcgccaacaa attcaggctg
    13801 aacgttccgc agatcccctg ctttatgcgt tttcactgct gtcttgctaa cgctttctgg
    13861 ccggttttcc cggccttatc gacttcgtaa actatcatgc aaaccctcat caaaacactc
    13921 gtcactcacc gcgtaaaacc gtttgtcagc gtcgtcacgc caacctggaa ccgcggcgca
    13981 ttcctgccgt acctgcttta tatgtaccgc tatcaggact acccggcaga ccgccgcgaa
    14041 ctcatcatcc tcgacgactc cccgcaaagc catcaacaca tcatcgaccg cctgaccaat
    14101 ggcacgccgg aatccttcaa catccgctac atccatcacc cggaaaaact gccgctgggc
    14161 aaaaaacgca atatgctcaa cgaactggcg cgcggcgaat acatcctctg catggacgat
    14221 gacgactact actcgccaga taaaatctct tactccatcg ccatgatgca aaaacaccgg
    14281 gcgctcatct ccggttccga ccagatcccc atctggtaca gccacatcaa ccgcattttc
    14341 aaaagccgca gctttggccc acataacatc ctcaatggca ccttttgcta tcaccgtaac
    14401 tacctgaaaa agcaccgtta cgacgacgat tgtaatttag gcgaggagaa aagtttcacc
    14461 gacaatttca gcgttaaccc gctgcaactg cccggcgagc gcacgattct gtgtatctcg
    14521 cacagccaca acacctttga taaagacttt atcctcgggg ccagcacgcc tgtaaacgcg
    14581 gtgcttacgg atatcgtcag cgatccgctg ctacgcaacg cttatctggg tctgcataac
    14641 gccactcatt atcaggcgat ccaccatcag gcgatcgatc aggtcgtatt gatcaatctg
    14701 gataaacgcc cggatcgctt acagcagatc cgtgaagaac tgacattgct gcatattccg
    14761 cctgagaaga tcacccgcct tgccgccagt gaaaatgaaa acggtcaacg gggccgccag
    14821 caatcacact tgcaggcgct gcgtctggcg caacagcacg gctggcaaaa ctacctgttg
    14881 ctggaagatg acgcggttat tctcaaacag gagaaacata ttcaggtgct gaacgcgctg
    14941 ctggcgagtc tggcgaaaat tccctggcag gtgatgatcc tcggcggcga aatttcgcag
    15001 ggaacgatgc taaaaagttt gcctgggctg gtgcacgcgc gggactgccg caaagtgtgc
    15061 gcttatctgg tgaacagccg ctactacccg cagctggcgc agcagatgaa caacgatgag
    15121 cattcgctgg aagacggctg gcagccgctg ctgcacaccg acaaatggct ggcctgttac
    15181 ccgagccttt gttatcagcg acagggcttt agtgatatcg agaaaaaagc gactgataac
    15241 attaaccact acttcaataa gttacccgta gccaccaagc catctacatt acctatcgcc
    15301 gataccatcg gcttttttat ggaaacctca ttccactacg cgctctaccg cccaatcatc
    15361 accgccctgc aggcgcaggg gcagagctgt acgctggtga tcaatgaccg cgtatttaaa
    15421 ccatttctgg atgaaatgct ggcgacgcta aaaagcatcg acgatccgca gctcaaagga
    15481 atgcgcttat ctgaaatgca ggcacacggt cagcgagtga aatgcctggt cagcccctat
    15541 cacacgcccg cgctcaatgg cctttcaacc ctgaacattc gcgccatgta cggcttagcc
    15601 aaagaaacgt ggaaccacgc cgactggaac cgtttttatc agcgcatttt gtgttacagc
    15661 cactacagcc agcgggcgct ggcacatttc ggcagcgcaa aagtggtcgg caatccgcgc
    15721 ttcgacgcct ggcataacgg cacgtttgac cgcgctctgc cggaaaatat ccaacccgat
    15781 tcccataaac ctacggtgct ctacgccccc acgtttggcg cattaagctc cctgccccac
    15841 tgggcagaaa aattgggccg cttaagcggt gatgtgaacc tgatgtgcaa actgcaccac
    15901 ggcacttgct cgcgcccgga agaagctgca tcgctggcac tggcgcgcag gcatttaaaa
    15961 cagcgcaccg actctgcccg ccacacgctg gcgctactgg caaaggccga ttacgtgctg
    16021 accgataaca gcggctttat tttcgatgct attcacgttg ataagcgggt gatcctgctc
    16081 gactttccgg gcatgacaac gttgctcgac ggtgagaaaa gttattccac gcccgaaagt
    16141 gccgaccagc aaattcgcga aatattgccg gtggctcatg atgttgcgga attacgttat
    16201 ctgctatcag aggcgtttga ctggtgtgcg gtacaggggc aactggcgga aattcgtcat
    16261 cactattgcg atgcgtttat ggatggcaag gcgggagaac gggcggcgat ggtgattatg
    16321 gaggcgctgg ggtaaaccgc gcaagtgcct gatgcgcttc gcttatcagg cctacgcgcc
    16381 cctgcaatat agtgaatttg cgatgatttg tcggccggaa aaggcgttta cgccgcatcc
    16441 ggcacgaaca gtgcacttat ctacccttcg atttcaaata catcgcttta tggctggtta
    16501 atcggtaaaa gttgcacatt ttgatagcga gaagagctgt ctttatgaat tccgaaaagc
    16561 gcccttctac cctctataac cattcttttt tacctgtatt ggccgcaaca tacctcaccc
    16621 acgaagtggt tgtagcaatt cgtgggctaa aggtccgcat tatatgaaac gggagggcgg
    16681 tagcatttct gatattacat gcctcaattt tgctgaacta ttggctatcc tggacctcaa
    16741 aaatctggtc ataagataga ttttttaagg gaatacggag taaaggtgtg gaaaa
//
LOCUS       DACADA010000100        16721 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.102, whole genome shotgun sequence.
ACCESSION   DACADA010000100 DACADA010000000
VERSION     DACADA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.102"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            14..1219
                     /locus_tag="ISB17_003175"
     CDS             14..1219
                     /locus_tag="ISB17_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1981074.1"
                     /translation="MVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQKRGVVSRHH
                     GGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGKVCVLGSFNV
                     DIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKIGRDQFNHFA
                     RNHIESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSDNDIIQLSHF
                     IAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPFVDIVTPNET
                     EASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCAHIPAFSAVA
                     VDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPDISLVDARIQ
                     QQNVAIQTF"
     gene            1245..2168
                     /locus_tag="ISB17_003176"
     CDS             1245..2168
                     /locus_tag="ISB17_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1981075.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            2223..3176
                     /locus_tag="ISB17_003177"
     CDS             2223..3176
                     /locus_tag="ISB17_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HAP1981076.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
     gene            3225..3662
                     /locus_tag="ISB17_003178"
     CDS             3225..3662
                     /locus_tag="ISB17_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HAP1981077.1"
                     /translation="MRIIPITPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPEGILF"
     gene            3672..5159
                     /locus_tag="ISB17_003179"
     CDS             3672..5159
                     /locus_tag="ISB17_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HAP1981078.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            5343..6107
                     /locus_tag="ISB17_003180"
     CDS             5343..6107
                     /locus_tag="ISB17_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1981079.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6221..6919)
                     /gene="thiQ"
                     /locus_tag="ISB17_003181"
     CDS             complement(6221..6919)
                     /gene="thiQ"
                     /locus_tag="ISB17_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAP1981080.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(6903..8513)
                     /gene="thiP"
                     /locus_tag="ISB17_003182"
     CDS             complement(6903..8513)
                     /gene="thiP"
                     /locus_tag="ISB17_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAP1981081.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQVLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(8489..9472)
                     /gene="thiB"
                     /locus_tag="ISB17_003183"
     CDS             complement(8489..9472)
                     /gene="thiB"
                     /locus_tag="ISB17_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAP1981082.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9503..9602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9636..11291)
                     /locus_tag="ISB17_003184"
     CDS             complement(9636..11291)
                     /locus_tag="ISB17_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAP1981083.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLTEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11380..11511
                     /gene="sgrT"
                     /locus_tag="ISB17_003185"
     CDS             11380..11511
                     /gene="sgrT"
                     /locus_tag="ISB17_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAP1981084.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11618..12223)
                     /gene="leuD"
                     /locus_tag="ISB17_003186"
     CDS             complement(11618..12223)
                     /gene="leuD"
                     /locus_tag="ISB17_003186"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAP1981085.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12234..13634)
                     /gene="leuC"
                     /locus_tag="ISB17_003187"
     CDS             complement(12234..13634)
                     /gene="leuC"
                     /locus_tag="ISB17_003187"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAP1981086.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13637..14728)
                     /gene="leuB"
                     /locus_tag="ISB17_003188"
     CDS             complement(13637..14728)
                     /gene="leuB"
                     /locus_tag="ISB17_003188"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAP1981087.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(14728..16299)
                     /gene="leuA"
                     /locus_tag="ISB17_003189"
     CDS             complement(14728..16299)
                     /gene="leuA"
                     /locus_tag="ISB17_003189"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAP1981088.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIDKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16391..16477)
                     /gene="leuL"
                     /locus_tag="ISB17_003190"
     CDS             complement(16391..16477)
                     /gene="leuL"
                     /locus_tag="ISB17_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAP1981089.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         3880 a         4161 c         4431 g         4249 t
ORIGIN      
        1 ccgtcgtgaa tatattgtcc ggttacttcg caacaaggat gagtgcagtg ttagcgattt
       61 agctaactat ttttctgtta ccaaagagac gattcggaca gatttagcgt ggttgcaaaa
      121 gcgtggtgta gtgagtcgtc atcatggagg cgtctcattg aaaaaacatt taatgcaaaa
      181 tgcgctttcg caacaagagt atgtcgatat gagtttgctt atcaagcaac aacagcaggg
      241 tattggctat ttaacctcgc ttgatgataa aggacgccga atggtaggta aagtttgtgt
      301 gttaggatca tttaacgtcg atattgtggc gaaagtgtat cgcttcccga atgaaggcga
      361 aacgttagtg gccagggaaa ctaccatggg gccggggggt aaaggcgcga atcaggcact
      421 agccgctcat cgggcaggtg cgcaaataca cttcgcctgt aagattggtc gcgatcagtt
      481 taatcatttt gcccgcaatc atattgagtc cattggtatt ggttcattta ctttttatga
      541 gtctgaacta tctaccaccg gttgtgcggt catttatgta aatgatgaag gtgaaaacat
      601 gattgctatg tctccagggg caaatcatga attaagtgat aacgatatta ttcaactttc
      661 tcactttata gccgagtccg atgtattcat tgtacagatg gaaaataatc ttgccgctac
      721 tcaactggca ttaaaatgtg ctcaaaaaat gcaggtgaca acgattttaa atcctgcacc
      781 gtggtccagt gatgtcgcca cattattacc ttttgttgat attgtgacgc caaatgaaac
      841 cgaagcatcg gcgatgtcag gtatggtaat tgacaatatt tctgatgctg tgaaggctgc
      901 gaagcatatt tataatgccg ggcaatgttc agtaattatt acgatgggaa aacgaggtgc
      961 attaatttat gacgggcaac aatgtgcgca tattccggca ttttcggcag tcgctgtaga
     1021 tacaacaggt gcgggtgatg cgtttaatgg cgcgctcgcc gccgcactgg cgaagggaga
     1081 atcacttgtt aaagcagcct ggtatgccag cgctttcgct tcgctggcag tagaaaaaga
     1141 aggggcagcc aatatgcctg atatctctct ggtggatgcc agaattcagc agcagaatgt
     1201 tgccattcag actttctgat gtttcactga acgaaggatg aaatatgaaa acaattgaag
     1261 ggattattcc ggtaatgcta acgcctttta cggcggataa taaaattgat tacctgggtc
     1321 ttaaacggct aattgactgg tatatcgaaa acggtgccga cgcgttattt gccgtgtgtc
     1381 agtccagtga aatgcagtat ttaactttag aagagcgcgt ggagctggcg agctttgtgg
     1441 ttgaatatag ccaacaacga gtaccggtta ttgcgtccgg gcatatttct gatgatctgg
     1501 aacagcaaat tcaggagctc aatgctatgg cggcaacggg cattgatgcg ctggtgttag
     1561 tgacgaatca tcttgatcca cataacgaag gtaccacggt attcctccgt acgcttgatg
     1621 cgctattgat gtcattaccc gaaagtatgg cgctgggtct gtatgaatgc ccggccccgt
     1681 atcgccgctt attaaccgat gaggaactgc tttactgcgc acaaagcggg cgttttcaaa
     1741 tcttaaaaga cgtcagttgt gatctaccta cagtgacgcg acgggtgcaa ttaactcagc
     1801 acacgccaat gacgattgtt aatgctaatg ctgccattgc ctgggaggcg atgcttgcag
     1861 gttcgaaagg tttttgtggc gtctttgcca actttcatcc tgacttgtat cactggcttt
     1921 ggcttgaagg aaaacaacac cctgaagagg caaaacaact cgcctggttt ttatcgctga
     1981 gtgccgtcag tgaaaatatt ggctatccga aaaatgccaa aatatttcat cagcagcgag
     2041 gaacatttga ctgtgttcat tgccgggtaa cggctgacga tgtgctgaaa aaatattggg
     2101 ggctggaggt ggtgctaaaa caaattgctg atgccgcaga tttccagcgt caacaactca
     2161 aatattaata tccgacagca cttgctgtta ttaaaaaata attaagacag gagtctacaa
     2221 atatgaaacg aaccctgatg agttgtgccg ttgcttcact gctggtttca ggttccgcgc
     2281 tggctgcatg gccagaaaaa ccaatccaaa tcattgtccc ctggggcgcg ggaggaaata
     2341 ccgatacggt tgcacgcctg gtggcagaag ggctgcaaaa agagttgaaa gtgaatgtca
     2401 acgtggtgaa tcgcacggga ggtgctgggg tggttggcca tgatgccatt gcgaaagcta
     2461 aagcggatgg ctataccctg ggaattgcga cggtagaaat caccatgatg caccatcagg
     2521 ggatgacggc gttaacgtat cagaactaca cgccaattac acggctggca gtgaatctgg
     2581 gaggcgtgca agtcgcagca aattcgcagt tcgaaaatat cgtccagatg accgactaca
     2641 tcaaggcgaa tccaggtaag ttaaaagcat cgggtagcgg gttaaattcc ggctggcatc
     2701 tgaacttgat aggtatgctc gacagtatgg ggcttccgcc ttctgccgtg acctgggtgc
     2761 cttctgaagg ttccagttct gcactgatgg aactggtttc cggtggtata gattttacaa
     2821 cgtcatcacc aggcgaagcg aaaagtatgg tcgatgccaa aatggtgcgt aatttagcga
     2881 ccatggcaga aaaatcagca gggctttata aagatcttcc agtattccaa caagcaactg
     2941 gctataaata tcatttggcg acatggaatg ccttagtggt tccggcgggt actcctgaag
     3001 aggtctcaca aaaattaact gccgctatgc aggcagtgtt tgcgcagggg aaattacaag
     3061 aattcgctac caaacagggt ttcgaagttt acccgctgta tggcaaagag atgacgacat
     3121 ttatggcaga tgaagatgcc agatacggaa aattaatagc caaaatcaaa ggttaatcag
     3181 acagggcggt gaatatatcg ccgccaaatt acagggatag cattatgcgc ataataccga
     3241 taacgccgtt atttctgctg atttttgcca tgtttatttt tggctggagc ataacgcaat
     3301 atggaggcat atcgcagtat ggcgctggat ttatgccttg catcatcagt ggattgttgt
     3361 tgtttttagc ggctattgat gtagcgatag atattaaaca ccggcaccgt aacaaagttt
     3421 ctttaacttt tgatgaagtg aaagcacttt tactggttat tacagtggta ctactgtttg
     3481 tattactgtt agatattctc ggctttgtga tttgctcatt tttgttgctt tttattttaa
     3541 tgaaaatacg aggtgtcaga cttttgcatt ccgttatcgt ttcgatatcg gcatctctta
     3601 ttatctggtt catttttgcc agagtgttgt tggtcgcctt tcctgaagga atcttgtttt
     3661 aaggatgcaa tatggatatt ttgattcagg cactaacgta tattaatttg aatgccattc
     3721 tggccattat tgctgctacg gcctttgggt tatttgtggg ggccatccct ggattgaccg
     3781 caacgatggc ggttgcacta atggtgcctt ttaccttctt tcttgatcct attcccgccc
     3841 tggcattaat gattgccgtt ggtgcgtcat ccatttatgc cggagatatc cccggcgcac
     3901 ttttgcgtat accagggaca cctgcttcgg cggcttatgt tgctgattca aatgaactgg
     3961 tcaagcaagg gaaggtgaat cgcgtattgg gacttgggct ggtgagttcg gtcattggtg
     4021 gcgtgattgg atcacttatt ttgatgttgg ctgcgcctgt actggcgaaa tttgccctta
     4081 attttagttc ttatgaatac acatggcttt ctcttttagg cttaacttgc gcgactctgg
     4141 ttgtgggtaa tcaaccactg aaaggcatgg tgaccttatg tttgggatta ttactggcgt
     4201 gtatcggtta tgatcaaata tccggagttc cacgttttac gtttggtcaa atcagcctgt
     4261 tacaaggggt gaactttatc ccggcgatga ttggtctgtt cgcgatatcc ggcgcaattg
     4321 aatattatcg tgacaaaatt agccaggtaa gtggtgttgt gccacaaacg caaacgggga
     4381 ttaatttatt caagggaatg gggcaggtga tttggtcacg aaaattaaat cttggccgaa
     4441 gtagtattat cggtacgctt atcggagcat tacccggcgc tggagcggat attgctgcgt
     4501 ggataagtta cgccatttca aaaaaattct ctcgccagca ggagttatat gggcatggat
     4561 ctgaggaggc aattgttgat tcctcgagca gtaacaacgc cagtctggcg gggagttgga
     4621 tcccttcatt agttttcggt attccgggcg attctgccgc tgcaattatc atcggcgtgc
     4681 tctatatgaa ggatatgaat ccagggccga cattatttct gttccaggca gataaacttt
     4741 atgccgtatt tatcttgttt ctgatcgcca atattgcatt actaccgtta gccacaattg
     4801 cggtcagttt catcaaacgc atcatctgga ttgataaagc gattctgtac ccgattattc
     4861 tgattttcag tattgtcggt gcatttgcta tcgataactc gggtgcttct gtcgttgtca
     4921 tgttggtcat gggggttctt ggatactggt tacaacgtaa ggaatacccg gtttcgccca
     4981 ttattctggg gatgattctt gggccaatgt tggaaaaaaa cttattatct tcaatgatta
     5041 agtctaatgg cgaatggctg gcgtttgttg agcgcccggt atctatggca ctggctgtct
     5101 gtttcttttt ggttgtcgcg ttgcaaggac gcaatatcta ccgttcattt agccgttaat
     5161 ccgttgtttt cagaccatcc aagttgtgga tggtctgcct tcccctaagt aaaatcctgc
     5221 gtgactgcta aatttatact ctaaatgatt cgcgttgcag gaatatattc atacaaagaa
     5281 tagattgacc tgaataccac cactttgcac aatccacgac gaattcatca accgggcaca
     5341 ttatgcaagc attgctggaa cactttatta cccaatccac cgtgtattca ttgatggcgg
     5401 tggtgttggt ggcctttctg gagtcgctgg cgctggtcgg tttgattctg cccggtacgg
     5461 tgctgatggc ggggctggga gcgttgattg gcagcggtga gttaagtttc tggcacgcct
     5521 ggctggcagg gatcgtcggt tgcctgctgg gcgactggat ctccttctgg ctgggctggc
     5581 gttttaaaaa gccgttgcat cgctggtcat ttctgaagaa gaacaaagca ttactcgata
     5641 aaaccgaaca tgcgctgcat cagcacagca tgttcaccat tctggtcggt cgttttgtcg
     5701 gcccgacgcg tccgctggtg ccaatggtgg cagggatgct ggatctgccg gtggcgaaat
     5761 ttattacgcc aaatattatc ggctgcctgt tatggccacc gttttacttc ctgccgggga
     5821 ttctggcggg tgcggcgatc gatattcctg ccgggatgca gagcggtgag tttaaatggt
     5881 tgctgctggt gacggcggta ttgttgtggg ttggtggctg gctgtgctgg cggttatggc
     5941 gcagcggtaa agcgactgac cgtttgagtc attatttgtc ccgcggtcgt ttgttgtggc
     6001 tgacgccgtt gatttctgcc atcggcgtgg tggcgctggt ggtgttaatt cgccacccgt
     6061 tgatgccggt gtatatcgat attttgcgta aagtggttgg ggtttagagg atagccttgt
     6121 gcgggttgcc tgatgcgacg cttgctgcgt cttatcaggc ctacaaaacg cactacctgt
     6181 aggtcggata aggcgttcac gccgcatccg acagtgcata ttaaccttta attcccaaca
     6241 gcgccgaagc actcgcctta ccgctcaaca actcatcggt cttaccctgc caggcgatac
     6301 gcccgtcggc gaccaccacc gagcgcgtgg cgatccgcgc cgcatcttcc acactgtgcg
     6361 acaccatcaa gagcgtcatt ttttgctgtt ggcagctcga actcaccagc gtcagcatct
     6421 cctgacgtag cgccggatcg agtgcggaaa acggttcatc gagcaataaa atgggctgtt
     6481 cgcgtaccag acaacgcgct aacgccacgc gctgtcgctg accgccggaa agctcgccag
     6541 gtaaccgcgc cattaaatta tcaatcccca tctggtgggc aatcgcgtgc attttcttct
     6601 gctgcgccgc atttagtttc aaccccggat ttagccccag cccgatgttc tgcgcgaccg
     6661 tcaggtggct gaacaggttg ttctcctgaa acagcattga caccggacga cgtgacggtg
     6721 gcgttgtggt gtgatctacg ccgtcgagag tcagcaaacc gctggctggc gtcagaaaac
     6781 cggcgatcaa atttagcagg gtgcttttac ccgcgccgct tggcccgagg atcgccacct
     6841 gctcgccgcg ttccaccgtt aagctaaaac gcatcggcaa atggtggtaa agccaggtga
     6901 tatcagtcag ttttaacatt tcgccccggt attttttcaa tcacggtaaa cagcagaaaa
     6961 cagagcagca gcagaattaa cgcggtgacc gcgccgtcct ggctgcgata ggagccaatt
     7021 tgctgataga gataaaacgg cagggtacgg aaatcatcgt taccgaacaa tgccaccacg
     7081 ccaaaatcac caatcgacag cacacaagca aaggccagcg cctgcgccag tggacgtttc
     7141 agagcgcgca gctccaccac ttttaagcgt gaccagcctt caatgcccag cgactgacac
     7201 aacatgctgt agcgggcggt gatatcgcgc atcgggtttt ccagtacttt cagcgcataa
     7261 gggattgcca ttaacgcatt ggtgaaaatc acaatgccgt cagcagattg tggcaggccg
     7321 atagtgttgt tgagcagtaa aaagaagccc gtagccagta caatccccgg catggcgagg
     7381 atcaacatgc cgctcatctc cagcgcctga cccgccagca ttttctgccg tgcccgaagt
     7441 tcgcgactgc tccatagcag catcatggtc agcactacgc acaatacgcc tgccgccagc
     7501 gcaatacgca acgaggtcca cagcgcctgc cacagcaccg gttgtgccag cacttccggc
     7561 aactggcgat ttaccccatc gacgatcacc gccagtaacg gtggcagcaa cagcagcagc
     7621 gccagcacaa ttaacgccgt gtcgcaaatg cgactatgca gacgatcgtc cgggtcgcgc
     7681 cagccttgca gcagtgtggt accgggcgca atggccttac tcaatcgctg actcaacagc
     7741 accagcccga ggcagcacac catctggatc agcgccagca ttgccgcgcg ggcaggatcg
     7801 tagtcgtaac tcagcgcctg atagattgcc agctcgatag tggtcgcctg cggaccgccg
     7861 cccagcgata gcacggtggc gaagctggcg aaacagagca taaagataag cgcagcaacc
     7921 ggcgggattt gtcgccgtaa ccacggccat tcgacgaagc ggaagaagtg ccagccgcgc
     7981 atcccaagtt gtgcggcaag ctgacgttgt tcgccgggaa tgttttccag tgcctggagt
     8041 aataaacggc tagccatcgg cagattaaaa aacacatggg ccagcaaaat gccttgcagg
     8101 ccgtagggcg aaaaggtcca ctccagacca agtgattgcc agagcgaggc cagccagccc
     8161 tggcgaccat agacgctaag aatgccgaaa acggcgacca gcaccgggag gatcaaggtc
     8221 attgcgcaca gacgcaacag cacctgtcgc ccaggaaagc gcctgcgata gagcgcgcgg
     8281 gcgaggaata ttgcgggtac gacagagagc agcgccgaga gaaacgcctg ccagaaggag
     8341 aagcgcacca catgccacag atagctgtcc tgccagactg ccacccagtc accctgcggc
     8401 gcgttccacc acagggcgag aaacgccgcc agcgcaaccg ctaccaccag tgtggcggcg
     8461 cttattcctg gaattaacca gccgggaatt aacggctgac ggcgcgttgc cattcgctaa
     8521 tccatgcctg acgttgtgcc gccacttcgg cgggcgtgaa ctctagcgtg gtggctggtt
     8581 tggtcaattg ttcaaatcct gcaggcagag tgacgtttgc taccggatac atccagttgc
     8641 cggttgggat cgcattctga aaagccggag aaaccataaa ctggaggaat ttttgcgcca
     8701 gctctggctg cttgctggca gcggtgcggg cggcaacttc cacctgcaga tagtgacctt
     8761 cgctgaagtt cgcggcggcg tagttatctt tcttctcttc gagaatgtga taagccggag
     8821 aggtggtgta actcagtacc agatcgcttt cgccttttaa aaacaggccg taggcttcgc
     8881 tccagccttt agtgaccgtg acagttttct tcgccagttt ctgccaggct tgcggggcgt
     8941 tatcgccata gactttttgc atccatagca acagacccag acctggtgta ctggtacgcg
     9001 gatcttcata aatcacccgc cagttttgat cgctctcaac cagttctttc aggctttgcg
     9061 gcggattttt cagtttgttc ttgtcataga cgaaggcgaa atagccatag tcaaacggca
     9121 caaaggtgtc atttttccag ccgccgggaa cgtttacagc ctctgtcgcg acgccgcttt
     9181 tggcaaacaa cccggttttg ctggcggcgt ccagcaggtt gttatccagc cccagcacca
     9241 catcggcttt gctgtttttg ccttccatcc gtagacggtt gagcagcgaa acgccatctt
     9301 ccagcgccac cagtttcagt tcgcaattac agtctgcttc aaaggctttt ttgactaccg
     9361 gaccaggtcc ccagtcagcg gcaaaggaat cgtaggtata aacagtcaga acaggtttag
     9421 cgaaaacagg cgctgtgcac agcaacagca ggggaagaca tttttttaac actttgcacc
     9481 tcacaaaaga gtggcaaagg acttgagaag gagcctcaaa tcccttcgcc ggcgttatcc
     9541 ggatcaggtt cgacgggtat tttctcagcg cacgcggctg cgtggcaccc cgttgagaac
     9601 ggcgttagtg tagtgatttt gttatcaacc agcaatcatg gatccggtgg tgcaaaccac
     9661 gctgatttaa aatcgaacca gccgagggtg ttcatgcgca ggccgcgcat actgcgttgc
     9721 ccctgaatga tcagccagtg gtggataagt ggcaccattg ctttgctggc gaccagttgc
     9781 tggcaccagt tcgccaggtt catttcgcca ttgcgccagc gggcggcgtc ggcttgccag
     9841 tcgatcggaa tacagtgttg gagtagcggc acctcgcaca agtgcgcgaa cagcgaaaaa
     9901 tccagcggca gcgtaaagtt ggcgctgtta agccagatat cgctctcgat ctctccttca
     9961 tgccactgat cgtagctgat ctctttgatt tccagcgtga cctggtgact cgccagaatc
    10021 tgctgcatga tcccggcaat cactcgatgt tcaatatgat cctgataaaa ggtcagggtg
    10081 agatgttcca gcccggcggg tttttcgcaa tgtgtcgggc gggcatggtg ccagcggggg
    10141 agcagtccat aagccgggaa ccagagttgc tggtattgtt cctcggcgaa atagaccaga
    10201 ttcgtcggag aaagcacata gcttacccag tccctgactt gctgattcgc cccgcggtgg
    10261 gtgcggttgt cgaacagtaa ataatagcaa ccttcctcca ggcggctttc aatctcttgt
    10321 tcctcgccct gcggcccttt cagcatcagc cctccggctg gctcgtcggc aatttccgtt
    10381 agaacccaga cgttaacttc gtcgattaat gcccggtaac cgaagaagtc atcgaatgcc
    10441 tgaattttca gttgattggt gctgttgcgg atcaccgcat acggaccggt gccgatggga
    10501 tggctggcaa agttactgag ggtttcccat tcgcgcggca ggatcattgc cggaacttgc
    10561 cccagcagta acggcaacca gcggtccggt tgcgtgagat ggatatccag cgtccagggc
    10621 gtcggcgaca caatgtcagc aatatgcgaa tagagcggaa gcgtattgat tcgttttaaa
    10681 gaggcgatca catcgtccat ttccagttca cgaccatggt gaaaatggac tcctggacgc
    10741 aaaaagaaac gccagtgaag cggtgatatt tgctgccagt ggtgtgcgat gtctgcttcc
    10801 agttccccat tttcctcatt tatgcgcgtt agcgaactga agatttgccg ggcgatatgg
    10861 gtttcagaac ggcgcaatgc gctgccaggt agcagattac gcaacggacg atagtagagc
    10921 acgcgcagga tgtgccgccc ctggcggaag ctgcggccca gatgagaaac cagcatttgc
    10981 cgcacagtcg ctttgtcgcc aaccaactgc actagttggt cgatacgatc ctgctccagc
    11041 aggtcttccg ctcgctgttg ctgaagcgcc agcccggtat agaggaatgt cagacgcgag
    11101 cgtttaccgc gcccgacttc cgcttcccac gtcagccagc cgcgatcctg catggtgttg
    11161 agcagggtgc gcatatgacg acgcgagcaa ctcagtaacg ctgccagttc gttgagcgtg
    11221 gtctcctgag atttaccctc gcagcattgc cacaggcgga tgaactgttg ttgcagacga
    11281 gcagatggca taaaagggga actcctgcgc aaaagacagc aattttattt tccctatatt
    11341 aagtcaataa ttcctaacga tgaagcaagg aggtgcccca tgcgtcagtt ttatcagcac
    11401 tattttaccg cgacagcgaa gttgtgctgg ttgcgttggt taagcgtccc acaacgatta
    11461 accatgcttg aagaactgat gcaatgggag gggagtcatt ctgactactg atttgcggac
    11521 atcatgtgtg actgagtatt ggtgttaatc gccacgccag cagtgattat ctgctggctt
    11581 ttttcttttg gcgattgagt gccggacggt gtacaaacta acgcataaac gccggttgct
    11641 tctcttcata agacgcaata gcatcgtcgt gctgcagagt caggccgatg ctgtccagac
    11701 cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa gcgataagtt ttctctcccg
    11761 ctttcacctc ttgcgcttcc aggtctacat caaaatgaat ccccggattg gctttcacca
    11821 gcgcaaacag ctcatccact tctgcatcgc ttaatttcac cggcagcagc tggttgttaa
    11881 agctattgcc gtagaagatg tcagcaaaac tcggcgcaat caccacctta aaaccgtaat
    11941 cggtcaatgc ccacggcgcg tgctcacgcg aggaaccgca gccgaagttt tctcgtgcca
    12001 gcaaaatgga agcgccctga tactgcggga agttcagcac gaagtccggg tttggctgtt
    12061 ggcctttttc atccagaaaa cgccagtcgt taaacagatg cgcgccaaaa cccgtacggg
    12121 tcactttctg caaaaactgt ttcgggatga ttgcatcggt atcgacattg gcggcatcca
    12181 acggaaccac caggcctgtg tgtttgataa atttctctgc catggtgtgc tccttattta
    12241 atgttacgaa tgtcggcgaa atgtccggtc acagccgcgg cggcagccat tgccgggctg
    12301 accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa agttacggtt gctggtggag
    12361 gcgcaacgtt cgcccggatt cagacggtcg ttattcatcg ccagacacat tgagcaacct
    12421 ggcaagcgcc attcaaaacc agcttcgata aaaattttgt ccagaccttc cgcttccgcc
    12481 tgggctttaa ccggaccgga gccgggaacc accagcgctt gtacgcctgg cgcgactttc
    12541 cggcctttgg cgatttccgc tgccgcgcgt aaatcttcaa tacgcgagtt agtacaggaa
    12601 ccaataaaca ctttgtcgat agccacttct gtcagcggaa tacccggttt cagccccata
    12661 taggccagcg ctttttctgc cgacgcgcgt tcaaccggat cggcaaacga agccggatcg
    12721 ggaatattgt cgttcacaga aatcacctgg cctggattgg tgccccaggt gacctgcggc
    12781 gaaatctctt ctgcttgcag agtgacaacg gtatcgaaag ttgctccttc gtcggtttgc
    12841 agggttttcc agtaggcaac ggcgtcgtcg aaatctttac ctttcggcgc atgcagacgg
    12901 cctttaacat agttaaaggt ggtttcgtcc ggcgcaacca gaccggcttt tgcgcccatt
    12961 tcgattgcca tattgcacag ggtcatacga ccttccatgc ttaaatcacg gattgcttcg
    13021 ccgcaaaatt ccaccacatg cccggtaccg cctgcgctgc cggttttacc aataattgcc
    13081 agcacgatat cttttgcggt aatgcccggc gcggctttgc cctggacttc aattttcatg
    13141 gtcttcgccc ggccctgttt cagggtttgc gttgccagta cgtgttcaac ttcggaagtg
    13201 ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg cggtatgtga gtcgccgcag
    13261 acaatggtca tccccggcaa ggtcacaccc tgttccggcc ccattacgtg gacgatcccc
    13321 tgatacgggt gattcaggtc atacagctcg acgccaaatt ctttgcagtt tttgatcagc
    13381 tcctgcatct ggatgcgcgc catttcaccg caggcattaa tgtctttcgt ctgggtagag
    13441 acgttgtgat ccatggtggc gaaggttttg cccggctgac gtaccgggcg accgtgggcg
    13501 cgcagaccat cgaacgcctg cggtgaggtc acttcatgca ccaggtggcg gtcgatatat
    13561 aacagcgggg tttcgttttc ggcttcgtac actacgtgag cgtcgaataa tttttcgtat
    13621 aacgtcttag ccatgattac accccttctg ccacatagcg ggcaatgata tcgcccattt
    13681 catcggtact aacggcggca gcgccacggg ctaaatcccc ggtgcgaatg ccttcttcta
    13741 atgcgcggtt aatggcgcgt tcaatggcgg aagccgcatc atcggcatcc aggctgtaac
    13801 gcagcagcag cgccagcgac agaatttgtg caatcgggtt ggcgatattt ttacctgcga
    13861 tatcgggtgc cgagccgccc gccggttcat acagaccaaa accttgctcg ttcaggctgg
    13921 cggaaggcaa catccccatt gagccagtga tcattgcgca ctcatcagac agaatgtcgc
    13981 caaacaggtt ggagcacagc aagacgtcaa actgtgatgg atctttaatc agctgcatgg
    14041 tggcgttgtc gatgtacata tgcgccagtt cgacgtccgg gtattccgtg gcgatctcgt
    14101 taacgatctc ccgccataaa atagaggatt gcagcacgtt ggctttgtcg attgaggtga
    14161 ctttgtggcg acgcttgcgg gcagattcaa atgcgatgcg ggcaatacgc tcgatctcaa
    14221 aacggtggta cacctcggta tcaaacgctt tttcatattg tccgctacct tcgcggcctt
    14281 ttggctgacc gaaatagatg ccgccggtca gttcgcgcac acacaggatg tcaaagccgt
    14341 ttgcggcaat gtcagcacgc agcggacaga atgcttccag cccctgatac agttttgccg
    14401 gacgcagatt gctgaataat ttgaagtgct tacgcaaagg cagcagtgcg ccgcgttctg
    14461 gttgctggtc tggcggcaaa tgttcccact tcggaccacc taccgagcca aacagcacgg
    14521 catcggcttg ctcacaacct tcaaccgtcg caggcggcag cggctgcccg tgattatcaa
    14581 tggctgcgcc gcctacatcg taatggctgg tggtgatacg catcgcaaat cggttgcgca
    14641 cggcatccag cactttcagc gcctgggtca tcacttccgg accaataccg tcccccggca
    14701 atacggcaat atggtaattc ttcgacatca cacggtttcc ttgttgtttt cgttgtgttg
    14761 agctttgcgt tgcaactctt tttcgacttc tgcggcacgc cagatattgt tcagtacgtg
    14821 caccatggct ttggcggagg actcgacaat atcggtggcc aggccgacgc cgtggaagcg
    14881 gcgaccgttg tagttggcga caatatccac ctgacccagc gcatctttac cgtgaccttt
    14941 ggcggtcagg ctgtatttca ccagttcgac gttatagtca gtgatgcggt ttatcgcctg
    15001 gtagacggca tcaaccgggc cgttgccgtt agcggcttct gctttaactt cttcgccaca
    15061 ggccagtttg acggcggcag tggcaatatc gttagaacct gactgcacgc tgaagtaatc
    15121 cagacggaaa tgctccggct cttcttgctg cttatcgatg aaggccagcg cctccagatc
    15181 gtaatcaaac acctggcctt ttttgtccgc cagcttcagg aaagcgtcgt acaggttgtc
    15241 taaattatat tcactttctt tatatcccat ctcatccatg cgatgtttca ccgccgcacg
    15301 ccccgaacga gaggtcagat tcagctggat ttggttcaga ccaatagatt ctggtgtcat
    15361 gatttcgtag ttttcgcggt ttttcagtac accatcctgg tggataccgg aggagtgtgc
    15421 gaatgcgccg ctgccaacaa tggctttgtt tgccgggatc ggcatattac aaatctggct
    15481 aactaactgg ctggtgcgcc atatctcctg gtgattgatg gcggtgtgga cgttgagaat
    15541 atccttacga actttgatcg ccatgatgac ttcttcaagg gaacagttac cggcacgctc
    15601 gccgatcccg ttcattgcgc cttctacctg ccgtgctccg gcatgtaccg ccgccagtga
    15661 gttgccgacc gccaggccca aatcgtcgtg ggtatgtacg gaaataatgg ctttgtcgat
    15721 gttgggtacg cgttcataca ggccgctgat gattccggcg aactcaaatg gcatggtgta
    15781 gcccacggtg tccggaatgt tgatggtggt ggcaccggca ttaatcgccg cttcgaccac
    15841 tcgcgccaga tcggcaattg gggtgcgtcc ggcatcttcg caagaaaatt caacatcatc
    15901 ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt tcgatcactt cgtccagcgt
    15961 gctgcgcagc ttggtggcga tgtgcattgg cgaagtggca ataaaggtat gaatacggaa
    16021 ggcttcggcg actttcaggg attcggctgc cacgtcgata tctttctcca cgcagcgagc
    16081 taacgcgcat acacggctgt ttttaacctg gcgagcgatg gtctgcactg attcaaaatc
    16141 acccggcgaa gagacgggga aaccgacttc catcacgtca acacccatac gctcaagggc
    16201 cagcgcaatt tgcagttttt ctttcacact caagcttgcc tgtaacgcct gttcaccgtc
    16261 gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg gtttgggtcc ttgtctcttt
    16321 ttgagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg gcgcgggttt ttgtttgact
    16381 gcgtgctggc ttaatgctgg atgccgctca ctcgtctacc gcgcaaagaa gatgcgttta
    16441 gtagtagtag accgataaag cgaacgatgt gagtcattaa atcagctcca gatgaatgcg
    16501 atatgctttt agagttactg gatacaaaaa cggatgtcaa ccctgaagca ataaaaacgt
    16561 cacgccattg tgagttcggc atccgtaaaa ttagctaatt gtgctgcggt ggttaaaaca
    16621 agcgatatta atttctgctt aaccgccgat gcttttcatc ggttgacata tttcagaata
    16681 attttttgca tctaatcaac gagggaaagg gggataaaat g
//
LOCUS       DACADA010000101        16347 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.103, whole genome shotgun sequence.
ACCESSION   DACADA010000101 DACADA010000000
VERSION     DACADA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.103"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(59..139)
                     /gene="shoB"
                     /locus_tag="ISB17_003191"
     CDS             complement(59..139)
                     /gene="shoB"
                     /locus_tag="ISB17_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAP1981090.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(560..940)
                     /locus_tag="ISB17_003192"
     CDS             complement(560..940)
                     /locus_tag="ISB17_003192"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP1981091.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(940..1671)
                     /gene="pdxJ"
                     /locus_tag="ISB17_003193"
     CDS             complement(940..1671)
                     /gene="pdxJ"
                     /locus_tag="ISB17_003193"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAP1981092.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1683..2411)
                     /gene="recO"
                     /locus_tag="ISB17_003194"
     CDS             complement(1683..2411)
                     /gene="recO"
                     /locus_tag="ISB17_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP1981093.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2423..3328)
                     /gene="era"
                     /locus_tag="ISB17_003195"
     CDS             complement(2423..3328)
                     /gene="era"
                     /locus_tag="ISB17_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP1981094.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3325..4005)
                     /gene="rnc"
                     /locus_tag="ISB17_003196"
     CDS             complement(3325..4005)
                     /gene="rnc"
                     /locus_tag="ISB17_003196"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP1981095.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4277..5251)
                     /gene="lepB"
                     /locus_tag="ISB17_003197"
     CDS             complement(4277..5251)
                     /gene="lepB"
                     /locus_tag="ISB17_003197"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP1981096.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5267..7066)
                     /gene="lepA"
                     /locus_tag="ISB17_003198"
     CDS             complement(5267..7066)
                     /gene="lepA"
                     /locus_tag="ISB17_003198"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP1981097.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7264..7743)
                     /gene="rseC"
                     /locus_tag="ISB17_003199"
     CDS             complement(7264..7743)
                     /gene="rseC"
                     /locus_tag="ISB17_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAP1981098.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7740..8696)
                     /gene="rseB"
                     /locus_tag="ISB17_003200"
     CDS             complement(7740..8696)
                     /gene="rseB"
                     /locus_tag="ISB17_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAP1981099.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(8696..9346)
                     /gene="rseA"
                     /locus_tag="ISB17_003201"
     CDS             complement(8696..9346)
                     /gene="rseA"
                     /locus_tag="ISB17_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAP1981100.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9379..9954)
                     /gene="rpoE"
                     /locus_tag="ISB17_003202"
     CDS             complement(9379..9954)
                     /gene="rpoE"
                     /locus_tag="ISB17_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAP1981101.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9951..10106)
                     /gene="rseD"
                     /locus_tag="ISB17_003203"
     CDS             complement(9951..10106)
                     /gene="rseD"
                     /locus_tag="ISB17_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAP1981102.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10362..11984
                     /gene="nadB"
                     /locus_tag="ISB17_003204"
     CDS             10362..11984
                     /gene="nadB"
                     /locus_tag="ISB17_003204"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAP1981103.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11969..12706)
                     /gene="trmN"
                     /locus_tag="ISB17_003205"
     CDS             complement(11969..12706)
                     /gene="trmN"
                     /locus_tag="ISB17_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAP1981104.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12838..14172
                     /gene="srmB"
                     /locus_tag="ISB17_003206"
     CDS             12838..14172
                     /gene="srmB"
                     /locus_tag="ISB17_003206"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAP1981105.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14205..15086)
                     /locus_tag="ISB17_003207"
     CDS             complement(14205..15086)
                     /locus_tag="ISB17_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1981106.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            15189..15776
                     /gene="eamB"
                     /locus_tag="ISB17_003208"
     CDS             15189..15776
                     /gene="eamB"
                     /locus_tag="ISB17_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAP1981107.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15832..16215)
                     /gene="grcA"
                     /locus_tag="ISB17_003209"
     CDS             complement(15832..16215)
                     /gene="grcA"
                     /locus_tag="ISB17_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAP1981108.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT         3965 a         4343 c         4006 g         4033 t
ORIGIN      
        1 gttattaaag gcgcgtccga ttggttcacc ggacgcacct taagtacgtt tccttgtgtt
       61 ataagaacaa aaggagcagc tgtaaaacag caatgatgat tttgatgacc cgtttaatca
      121 ggtatcggca atcagtcatt cgtttttcct taaacaagga atgcagccgt tcagattagc
      181 ccttacatct ccccaaaact gaacgtgcga gttattggaa tcgagtgctg cactccacac
      241 cagagctttg acgacaccac tcgtttcaat gggggaattc agtggcatgg tgtaaagcac
      301 agcaaaattg ccaataacga aaccgatttt aatgtactta tcaaaaagcg gtcacgttaa
      361 tcttttcatc aatgtcgatt tcttcgtaaa ggctcgcgtt ttttatgtca gagattgcaa
      421 gttgcttgta caggtcaacg aaattgatcc ataatcattt tcgttggaat cgtgtgctgc
      481 acaaaattaa agttaaaaag taaaaccccc gttccttacc agttcggggg ttttacttta
      541 taaagagaac ggtattattt taactttcaa taattaccgt ggcacaagca tagtggcgct
      601 catcggcaag cgttacatgc atatttacaa cgccgagctt ttccgccagt tttaatgcct
      661 caccccatag ccgtagccgt ggtttgccga gctcatcatt gaatacttca aactgattaa
      721 acgccagacc attgcggatc cctgtgccaa atgcttttgc cgccgcttct ttcacagcaa
      781 aacgcttcgc cagaaaacgc actggctggt ggtgcgtttt ccagatttcc cattcgttat
      841 cgcttaatac gcggcgtgcc aggcgctcac cggatcgggc gatcaccgct tctatgcgag
      901 cgatctctac gatatccgta cctaaaccta atattgccat tagccacgcg cttccagcat
      961 cagacgcttc atttctgcca ccgcatcttt cagtccggtc atcactgcac gaccaataat
     1021 ggcatgaccg atattcagtt catgcatctc agggatggcg gcaatggctt tcacgttgtg
     1081 ataggtcaga ccgtgtccgg cgttaacttt cagaccgaga ctagtggcaa aggtcgcggc
     1141 tttggcgata cgcgccaact cttgcgcctg ttcggcgtca gttttggcat cagcatagca
     1201 accggtgtgg atctcgataa acggtgcgcc aacctctgcc gcagctttga tctgctcttc
     1261 atcggcgtcg ataaacagag aaacctggat cccggcatct gccagacgtt tgcaggcatc
     1321 gcgcattttt tcacgctgcc ctgcgacgtc caggccgcct tcggttgtta cttcctgacg
     1381 cttttccggt accagacagc aaaaatgtgg cttcgtctca acggcgatcg ccagcatctc
     1441 ttcggtcacc gccatctcca gattcatgcg ggtatccagc gtctggcgca ggatgcacac
     1501 atcgcggtcg gtaatgtggc ggcggtcttc acgtaaatgc acggtaatgc cgtccgctcc
     1561 cgcctgctca gcaataaacg ccgcctgcac cggatccggg taagcagtac cgcgcgcgtt
     1621 acgcagtgtt gcaatatggt caatgttgac gcctaacagt aattcagcca tgacaatcct
     1681 catcattcat aatgtgtttt caccgttcgc ttaggcataa actgccggaa cagttccctg
     1741 ctctttaatg gtttaccgcc aagatacggc ttaagcgcca tgcgggtaaa gcgtttcgcg
     1801 gcgcgcagtg tgtctgcgtc aggaaattcc cgcgcgttta acgcttttaa ctgccttccg
     1861 gtgaacgttt tattgtcgat aacgacgctt gcgataaacc ctttttcttc gcgataacga
     1921 tacgtcatgg tgtcatctac cggctcgccg ctacccgcac aatgggtaaa attgacgcca
     1981 tagcccagat gcccgagcag tgccagttca aagcggcgca acgcgggttc tggcgtacca
     2041 gtgaccccgg caagagactg aatgcagtgc aagtaatcga aaaagagttc agagaagcgc
     2101 gtctcgtatt ccagtacgcg ggagagaagt tcgttgatgt acagaccgct gtaaagcgtg
     2161 ataccgctta atggcagcgc cagcgagaca gcttcagcac tgcgcagcgt tttgacttcg
     2221 ccacgcccgc caaaacgtag caagagaggg gtgaaaggct gtaatgcacc tttcagagta
     2281 gagcgtttag agcgtgcgcc tttggcaacc agacgcacgc gacccgattc ctccgtgaag
     2341 acgtccagca tcaggctggt ttcgctccac gggcgactat gcaggacaaa tgcgcgctgc
     2401 cagccttcca tcggagttac tcttaaagat cgtcaacata accgagactg cgcagtgcgc
     2461 gttcgtcgtc ggcccaaccg gatttcactt ttacccacag ctcaaggtga acaggcgctt
     2521 cgaacatttc ctgcatgtct ttacgcgctt caatcccgat ggttttgatc ttggcccctt
     2581 tgttgccaat gaccatcttc ttctgccctt cacgctcaac gagaatcaaa ccgttgatgt
     2641 cataaccgcc gcgttcgtta gagacgaaac gttcgatctc cacggtcacg gagtacggca
     2701 gttcagcgcc gagaaaacgc atcagttttt cgcggataat ttcagacgcc ataaaacgct
     2761 gtgagcgatc ggtgatgtaa tcttccggga agtgatgggt cgcttcaggc agatgcttac
     2821 gcacgattgc tgcaatagtg tcgacattca gcccggtttc ggcagagatc ggcacgatat
     2881 cgaggaagtt catctggctt gccaggaact gtaagtgtgg cagcagatcg gctttttcct
     2941 gcacattatc cactttgttc accgcgagga ttaccggcgc tttgccatcg cgcagtttgt
     3001 tgagcaccat ttcatcgtcc ggcgtccagc gagtgccttc tacgacaaaa atcaccagct
     3061 caacatcgcc aatggagctg ctcgccgctt tgttcatcag acggttaatg gcgcgttttt
     3121 cttccatatg caggcctggg gtatcaacgt agatcgcctg atacgcgcct tcagtatgga
     3181 tccccacaat gcggtgacga gttgtttgcg ccttgcggga ggtaatggag atcttctgcc
     3241 ccagcagttt gttcaacaat gtggatttgc caacgttcgg acgtccgacg atagcaataa
     3301 atccgcagta acttttatcg atgctcattc cagctccagt tttttcaacg cctgttcggc
     3361 ggcagcctgc tcagccttac gacggcttga acctgtgcca accaccggtt cactcaggcc
     3421 gctgacctgg cagtggatag taaattcctg atcgtgcgct tcgccacgta cctggactac
     3481 cagataagtt ggcagcggca gatggcgacc ctgcaaatat tcctgcagac gcgttttcgg
     3541 atctttttgt ttatcgcctg ggctaatttc gtccagacga gtttgatacc agttgaggat
     3601 taatttctcg acggtttgaa tatcactgtc gaggaatacg ccaccaatta atgcttcgac
     3661 ggtgtcggcg agaattgact cacgacgaaa tccaccgctt ttaagttcac ctggccctaa
     3721 acgtaagcac tcacctaact caaattcgcg cgccagttca gccagcgtat taccacggac
     3781 cagcgtggcg cgcatccggc tcatatcgcc ttcatccaca cgagggaaac ggtgataaag
     3841 cgcattggcg ataacgtagc tcagaataga gtcacctaaa aattccagac gctcgttatg
     3901 tttactgctg gcactacgat gagttaatgc ctgctgcaac agttcctgat gattaaaagt
     3961 gtagcccagc ttccgttgaa gccgattaat tacgatgggg ttcatgcgat accaataaat
     4021 aaatgcgtca acaattcagc acacgaaaca gacctgatat acatggctcg gctaactgct
     4081 tcactgcagt ttcagtttac cggtatatgg ggaccaacgc tgtttcgtgt gccgtggcaa
     4141 cctggaggtg ccaaccttaa acttcggggg aatattctat acacaacgac gggggatgtc
     4201 gttagccacg ggagatttat ctcataaata attcacgttg tcgccataac ggcgacaacg
     4261 tgaacgaaga tggctattaa tggataccgc caatgcgact taagcgcaca cctgtcggcc
     4321 attcgccttc ttgcttatcg aagctcatcc agatagccgt tgcccgaccg accagattcg
     4381 cttcaggcac aaagccccag taacgactgt ccgcgctgtt gtcgcggttg tcgcccatca
     4441 tgaagtattg tcccggcgga acaatccagg ttgccagttg ttgccctggc tgctggtaat
     4501 acatccccac ctggtcctgc gcaatcggca ctgtcagaat acggtgcgtc acatcaccca
     4561 gtgtctcttt acgctcggaa agacgaattc cattttcttt ggtttcgttt ttcggcactt
     4621 caaagaatcc gctggtcgct tccccaccat tacggcgtga gaaggtctga acgaaatcgc
     4681 tcggttccac gttggagtaa gtgaccggta gcgcgttttc acacgcctgg ccggaactgc
     4741 atcccggttg aatcgtcagc tcttttgaga ccggatcata agtgacttta tcgcccggta
     4801 aacccaccgc gcgcttgatg taatcaagct ttggatcttc cgggtattta aagaccacga
     4861 tatcgccgcg tttcggatga ccggtttcga tcagcgtttt ctggtagata ggatctttaa
     4921 tgccataagc aaacttctct accagaataa aatcaccgat taacagagtc ggcatcatcg
     4981 aacctgacgg gatctggaac ggttcataaa taaacgaacg cacaatcaat acgatagcca
     5041 gtaccggaaa aacagaagca ccggtttcca gccagccagg cttcggcgca acctttttca
     5101 gcgttgcttt atccagtgag tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc
     5161 gtttaggtgc gaaaaagaat ttatccacgc accataaaat gcccgtcacc agtgtggcaa
     5221 tcaccagaat cagggcaaac atattcgcca tgccaactcc taagggttat ttgttgtctt
     5281 tgccgacgtg cagaatggcg aggaacgctt cctgcggcag ctcgacgtta ccgatctgct
     5341 tcatgcgttt cttaccttct ttctgcttct gcagcagctt tttcttacgg ctgatatcgc
     5401 cgccataaca tttagccagt acgtttttac gcagctgttt cacggtggag cgcgcaatga
     5461 tgtgcgtacc aatcgctgcc tgaatggcga tatcaaactg ctggcgtggg atcagatctt
     5521 tcatcttctc caccagctcg cgaccgcggt tttgcgaatt accacggtgg gtgatcagcg
     5581 ccaatgcatc aacacgttca ccgttgatta atacgtctac acgtaccatg tcggacgcct
     5641 ggaagcgttt gaagttgtaa tccagagacg cataaccacg cgaggtagat ttcaggcgat
     5701 cgaagaagtc gagcaccact tccgccatcg ggatctcgta cgtcagcgcc acctgattac
     5761 cgtggtaaac catattggtc tgcacgccgc gtttttctac gcacagcgta ataacgttgc
     5821 cgagatatgc ctgcggcagc agcatgtgac actctgcaat cggctcgcgc agttcgtaaa
     5881 tgttatttac cgctggcagc ttggatgggc tgtcgacgta gataacttct cttgacgtgg
     5941 tttcaacttc atacactaca gtcggcgcag tggtgatcag atccagatcg tattcacgtt
     6001 ccagacgttc ctggatgatc tccatgtgca gcaggccaag gaagccgcag cggaaaccaa
     6061 agcccaacgc gctggagctt tccggctcat agaacagtga ggcatcgttc aggctgagtt
     6121 tacccagcgc gtcacggaag gcttcatagt cgtcggaact taccgggaac agaccagcat
     6181 atacctgagg tttgactttc ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca
     6241 gtgttaaggt atcgcctact ggagcgccgt ggatatcttt aatcgcacat accagccagc
     6301 ctacttcgcc acatttcagt tcagtgcggt caatctgctt cggcgtgaag atgcccagac
     6361 gatcggcgtt ataggtctgc ccggtgctca tgactttcac tttgtcgccc ttacgcaggg
     6421 tgccgttttt aatacggata agtgaaacaa cgcccaggta gttgtcgaac caggagtcga
     6481 taatcagcgc ctgcaatggg ccttccggat cgccttccgg cggcggaatg tcgcgcacca
     6541 agcgttcgag aacgtcttgc acgccaacgc cggttttcgc tgaacagcgc accgcgtcgg
     6601 tggcgtcgat gccgacgata tcttcaattt cttccgccac gcgttcagga tcggctgccg
     6661 gcaggtcaat cttgttcagt accggcacca cttcgagatc catttccatg gcggtgtagc
     6721 agtttgccag ggtttgcgct tctacgccct gcccggcgtc gaccaccagc aatgcacctt
     6781 cacaggcagc cagcgaacga gaaacttcat aggagaagtc tacgtggccc ggggtgtcga
     6841 taaagttaag ctgataggtt tcgccgtcag acgctttgta gtccagcgtc acgctttgcg
     6901 ctttgatggt aatgccacgc tcacgctcaa gatccatgga atcgagaacc tgcgcctcca
     6961 tttcacggtc agacaggcca ccgcagatct ggataatacg gtcagacagc gtcgatttac
     7021 cgtggtcaat gtgagctatg atcgaaaagt tacgtatatt cttcataaag tatgattatt
     7081 gtgccttaat gcccggttaa ccaggctttt agaagtcgct gttctgagct taacgtctgt
     7141 attaatagaa acgccgcatt ctacactaca acattgaggc gaggaaatgt tcataccgta
     7201 tggattgtgg tatatggaaa cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg
     7261 aaatcactgg ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggccag gcggcaatgc
     7321 cacgcttaag atgatcggtt gccattctgc ccgggcagca aacttacgcg aatagccgcg
     7381 cgcaatcagg aacccaccaa taccgccgag gatcgcacca cataatgctg cgacgtcaga
     7441 agcaaagagt agctgaaata gcgaagcgat gaggaataat cccaccagcg gcgacatata
     7501 aaccagtaat gcggagctaa gaaggctgcc ttcggcgatc cccaattcca ctttttgccc
     7561 cggcactagc ggctcatcac agggtacgac aatggtatgc gtggtttgcg ggccaagttt
     7621 attcaacaca cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg aggctttaac
     7681 atcacaactg accagcgcct gcccgttttc ccaggagacg acggtagccc actctttgat
     7741 cattgcgctg tcccgaactt aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt
     7801 tcaccaacaa tggtgatttc ggcgttatca cgtacgcttg tactgacggt tctgcgtccg
     7861 gtgcgcaaca tctgatcggt gctcgatggc gtagcgcggt taacgtttac cgagaagctg
     7921 aataatccgt cggaatagag acgtgattcg ataggcatgt tgtccatcgt cggtagcgga
     7981 cgtcgactac tggaaacttc gctaaaaccc tgtggcagcc aggttggcgt ccagctgaat
     8041 ttagcttttt caccaacagg aacagaaagc aacggcggca aatttgcctt cgccagcgtc
     8101 tgcatactgc tgccgatatc ctgattgacg ttaaaagcaa tcacgcgaaa ttgttccagc
     8161 gtttcaccat cgcgatcaag gagatcaacc cgcatcggta atttcgattc ggtgtccatc
     8221 cacacgatgt agctgtagcg tgtaccatct cgggcaacca cacgaatgac ttcgcaaaga
     8281 cgatcagcaa tacgcgtgcg cccgacagag ataaagtcgt agtaaggaga aaggcgtttg
     8341 aaatcggtat agatgagcga tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac
     8401 ggttcaagtc ccggttcaaa atagctgatt tcattgccgc gctgtaccac ttcccggcgc
     8461 gggccatcca tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa
     8521 cgcagagact caacaccctg tttattgatg ctgataaatg acagctcgta attcagtgac
     8581 tgactggcca ggttcatctg ctgtaataac gccccggacg cgggagtggc caaggcgtta
     8641 gcagagaata acaggctacc tgtcactaat gacatggcaa accaaagttg cttcattact
     8701 gcgattgcgt tcctaaagtt tgaattcctg gcacctgtac agcggcttgc tgagtttgtg
     8761 cctgctcaaa ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca
     8821 ttgcattaat gcgacgacgc tgctcctgta cctgctgctg ttgaccatta tttgcggtcg
     8881 cttcagaagg tactcccaga ctaaccgggc tggctttacc catcatcggc agtgtattaa
     8941 atactggcgt ttcgggctgc tgggacgttt cagattgccc attatagtgc tggacgccaa
     9001 cgataactgc aagcgatacg catgctgcta cgcccatttg ggtaagctgt gccgcccacg
     9061 gacgtacttt ctgccagaat ggcattttct gccattgatg cggcgcaggc tgggcttccg
     9121 ggatcaatgt cgccggttga cgtactggct cttcttcaat ggcggccatc acgcgtgaag
     9181 agatatcgaa atggagcacc tcgggagtat caccccgcat tgagtcacgg attaagtgat
     9241 agctttccca ggttttctgc atttctgggt tatgagccag ttcgttaagc agctcactat
     9301 ccagcgtttc gccatccatt aaagcggaaa gttgttcttt ctgcatgcct aataccctta
     9361 tccagtatcc cgctatcgtc aacgcctgat aagcggttga actttgttat caatagcttc
     9421 cctcgctcgg aagatacgtg aacgcaccgt acccaccgga caatccatga tagcggctat
     9481 ctcttcatag ctcaggccat ccagctcccg caaggttatt gccatgcgta aatcttccgg
     9541 gagggactca atagttcgga aaactatctg tctcagttct tctgacaaca ttaagttctc
     9601 agggttcgaa atttctttca acgcgccgcc actttcgaag ttttcagctt caatggcatc
     9661 cacatcactg gaaggtggac gacgcccctg agcaaccagg taatttttcg ctgtatttac
     9721 agcaatccga tacagccatg tataaaaagc gctatctccc cggaacgaat ccagcgcacg
     9781 ataggcttta ataaaagctt cttgtaccac atcgggaaca tcacccgacg gcacatagcg
     9841 ggaaaccaga ctcgccactt tatgctgata gcgcactacc agtaagttaa aggctttctg
     9901 atctcccttc tggacccgtt caaccaggac ctggtccgtt aactgctcgc tcatccgagg
     9961 taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac gccactccat tagctgcaat
    10021 ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt
    10081 tgtttgtcat gctgtagacg gatcattatc tatcattata agtctacaga atctgaacat
    10141 cgcattatct gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa cggccttttt
    10201 atttcaccac ctaatcctcc accagccagt aacttctctt tttctcgccg ccctgagcca
    10261 gcgtgtttag tcactgtaac aaatattaaa atagcaggtg tttatttgca caacatgatg
    10321 ttatgctgac caaacagtgt ttagtaaatt aaacaaagaa aatgaatact ctccctgaac
    10381 attcatgtga cgtgttgatt atcggtagcg gcgcagctgg actttcactg gcgctacgcc
    10441 tggctgacca gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat
    10501 tttatgccca gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg
    10561 tggaagacac attgattgcc ggggctggta tttgcgatcg ccatgcagtt gaatttgtcg
    10621 ccagtaatgc acgatcctgt gtgcaatggc taatcgacca gggggtgttg tttgataccc
    10681 acattcagcc gaatggcgaa gaaagctacc atctgactcg tgaaggtgga catagtcacc
    10741 gtcgtattct tcatgccgcc gacgccaccg gtagagaagt agaaaccacg ctggtgggca
    10801 aggcgcagaa ccatccgaat attcgcgtgc tggagcgcag caacgcggtt gatctgattg
    10861 tttctgacaa aattggcctg ccgggcacgc gacgggttgt tggcgcgtgg gtatggaacc
    10921 gaaataaaga aacggttgaa acctgccacg caaaagcagt ggtactggca accggcggtg
    10981 cttcaaaagt ttatcagtac accaccaatc cggatatttc ttctggcgat ggcattgcta
    11041 tggcgtggcg cgcaggctgc cgggttgcca atctcgaatt taatcagttc caccctaccg
    11101 cgctgtatca cccacaggca cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg
    11161 cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga ttttgatgag cgcggcgaac
    11221 tggccccgcg cgatattgtc gcccgcgcca ttgaccatga aatgaaacgc ctcggcgcag
    11281 attgtatgtt cctcgacatc agccataagc ccgccgattt tattcgccag catttcccga
    11341 tgatttatga aaaattgctc gggctgggga tagatctcac gaaagaaccc gtgccgattg
    11401 tgcctgctgc acattatacc tgcggtggtg taatggttga tgatcatggg cgtacggacg
    11461 tcgagggttt gtatgccatt ggcgaagtga gttataccgg cttacacggc gctaaccgca
    11521 tggcctcgaa ttcattgctg gagtgtctgg tctatggctg gtcggcggca gaagatatca
    11581 gcagacgtat accttatgcc cacggcgtca gtacgttacc gccgtgggat gaaagccgcg
    11641 ttgagaaccc tgacgaacgg gtagtaattc agcataactg gcacgagcta cgtctgttta
    11701 tgtgggatta cgttggcatt gttcgcacaa cgaagcgcct ggaacgcgcc ctgcggcgga
    11761 taaccatgct ccaacaagaa atagacgaat attacgccca tttccgcgtc tcaaataatt
    11821 tgctggagct gcgtaatctg gtacaggttg ccgagttgat tgttcgctgt gcgatgatgc
    11881 gcaaggagag ccgtggcctg catttcacgc tggattatcc ggaactgctc acgcattccg
    11941 gtccatcgat cctttctgcc ggcaatcatt acataaacag ataaaaagcc tgggtcagcg
    12001 ccgtatacgc ttcggaatag ttctggtctg gcccacgaat gactaagcga tcgctaaagc
    12061 actctcccgc ctgcggggag aatgccagca gcacccgatg cggcagtcgc gcttcgtttt
    12121 ccgccacatc cgtccgcaaa cgtaaatgcc agcccatact taatgccagt tcagtaaaac
    12181 cattaccaat ctgctctggc agcaccacgc agaaaaatcc ctcttcggta atgcactccg
    12241 ccgcacaagt cagcaacgat gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt
    12301 gaggtgtcgc gcactctact ccctgctgat agtaaggtgg gttgctgatg attaaatcga
    12361 agcgtactgt ctgctgtgtg atccactgca gaatatccgc cgtatggaca ttaatccgct
    12421 ctgcccacgg ggactggttg atattttcct gcgcttgcgc ggcagcttca ctttccagtt
    12481 caactgcatc aatcatcacg ctgtcatctg ttcgctgcgc cagcattaat gccagcaacc
    12541 cgctacccgc gccgatatca aggcaacgtt ttaccccagc taccggtgcc catgcgccca
    12601 gcaaaatgcc atccgttccc actttcatcg cacagcgatc gtgagcaaca aaaaactgtt
    12661 taaaagtaaa tccattacga cgaaacacgg atgtagactg tgacatgaaa ataaaacctt
    12721 gcaggaaaaa cggcgatagc accgggtgag aacaataccc gagaagcgat atccatacaa
    12781 acagatgaag attgcagccg taacgtctat aatcagcgcc ccacacagag gtagaacatg
    12841 actgtaacga ctttttccga acttgaactc gacgaaagcc tgctggaagc cctccaggat
    12901 aaaggtttca ctcgcccgac cgccattcag gctgccgcca ttccgcctgc gctcgatggc
    12961 cgtgatgtac tcggttctgc gccgacaggc accggtaaaa cggcggcgta tctgctgcca
    13021 gcgttgcagc acctgctcga tttcccacgt aagaaatccg gtccgccgcg tattttgatc
    13081 ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc atgcccgcga actggcgaaa
    13141 catacgcatc tggatattgc caccatcacc ggcggcgtgg cctatatgaa ccacgcagaa
    13201 gtgttcagcg aaaaccagga cattgtggtc gccacgaccg gacgtctgct gcaatacata
    13261 aaagaagaga acttcgattg ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt
    13321 atgctggata tgggcttcgc ccaggatatc gaacatattg ctggcgaaac gcgctggcgt
    13381 aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg cgattcagga ctttgccgag
    13441 cgtctgttgg aagatccggt ggaagtttct gccaatccgt ccacccgtga gcgcaaaaaa
    13501 attcatcagt ggtattaccg cgccgatgat cttgagcata aaaccgcgtt gctggtgcat
    13561 ctgttaaaac agccggaagc gacccgctca attgtgtttg tgcgtaagcg tgagcgtgtg
    13621 catgagctgg caaactggct gcgcgaagca ggcatcaaca actgctatct cgaaggtgag
    13681 atggtacaag gcaagcgtaa cgaagcgatt aaacgcttga ccgaaggccg cgtaaacgta
    13741 ctggttgcta ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag ccacgtcttt
    13801 aacttcgata tgccgcgcag tggcgatact tatttgcacc gtatcggacg taccgcgcgc
    13861 gccggtcgta aaggcaccgc gatttcgctg gtggaagccc atgaccatct gctgctgggc
    13921 aaagttggcc gttatattga agagccaatt aaagctcgcg taattgatga gttacgtccg
    13981 aaaacgcgtg cgccaagcga gaagcagacc ggcaagccat cgaagaaagt gttggctaaa
    14041 cgtgccgaga agaaaaaagc taaagagaaa gagaagccgc gggtgaaaaa acgccatcgc
    14101 gacactaaaa atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc accgcaaacg
    14161 acagaagagt aatctcaatg ccgggtttaa gcccggcatt gtttttatcc cggcaaaaaa
    14221 cactcttcaa cacactgaat aaaagtgtgc atcgccgggc taaccgcttt tccggcatgg
    14281 tgagcgcaca ttgccgtaat ggtctgcgac tgttcgccaa aaggcaattc aattaattct
    14341 ccgcactcca actcttttac taccgcaaaa cgcggcaggt aactgactcc gatatttgcc
    14401 gcgacacaac gcttgatgct ttcgatactt ataagctcaa tggtgttttc caccgtgatc
    14461 cgccgctggc gcagcgtgct ctcaaatatc tgccggaaga cacattgtgg ttcgttgata
    14521 ataaagctac aggcgttatg tcttcccggc tcagtaaaat cgacatctgc aatttgcggt
    14581 gaagccacca gcaccagcgg ttgttcaccc aactctcgtc ggttcagagc atcatcattc
    14641 cctacgcgat aaaaaacccc gacatcggcc tcatcattca gtagcgcatc acggatcacg
    14701 taacagttca gcgactgcaa cgataaacgc acttttggag cgcgttgccg aaaacgctgc
    14761 aacacctgcg gcattcgata agagagcaac gtttcgcctg aaataacgcg tagttccccg
    14821 tccggatccg actctttttt agcagcctca cgaagcgtat ccatcactcg ggtaagttca
    14881 tagatgtgcg gcagcagctt ttttccttcg cgggtaaggc acattcgtcg accaattttc
    14941 tcaaacaatt gtaccgagaa ctcctgctca agctgctgaa tatgaaaagt caccgtcgat
    15001 tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac cctcttccac cacggtttta
    15061 agcgtaataa aacggcgcag atccataacc ccaaacctat cgaaaatatc gaatttagaa
    15121 tgtaaaaaca ttcatttttt taaatgttcc gtgtcgggta ctgtctacca aaacagagga
    15181 gataacaagt gacaccgacc cttttaagtg ctttttggac ttacaccctg attaccgcca
    15241 tgacgccagg accgaacaat attctcgccc ttagctctgc tacgtcgcat ggatttcgtc
    15301 aaagtacccg tgtgctggca gggatgagtc tgggattttt gattgtgatg ttactgtgtg
    15361 cgggcatttc attttcactg gcagtgattg acccggcagc ggtacacctt ttgagttggg
    15421 cgggggcggc atatattgtc tggctggcgt ggaaaatcgc caccagccca acaaaggaag
    15481 acggacttca ggcaaaacca atcagctttt gggccagctt tgctttgcag tttgtgaacg
    15541 tcaaaatcat tttgtacggt gttacggcac tgtcgacgtt tgttctgccg caaacacagg
    15601 cgctaagctg ggtagttggc gtcagcgttt tgctggcgat gattgggacg tttggcaatg
    15661 tgtgctgggc gctggcgggg catctgtttc agcgattgtt tcgccagtat ggtcgccagt
    15721 taaatatcgt gcttgccctg ttgctggtct attgcgcggt acgcattttc tattaacgaa
    15781 aaaaagcgga agaggtcgcc ctcttccgct tagtaacttg ctacttaagc cttacaggct
    15841 ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg
    15901 aactgcataa ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc
    15961 atcttccaga gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg
    16021 aacttctggt ttcacttcta ctggaacttc acggtattca atgtcaccca gtttgcttac
    16081 tgcaaccact tcgtcttctg caaaacctgc tttcgcaacg atgcaacgcg cttcgccttt
    16141 ttcgctgtcc agcaaccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg
    16201 gatacctgta atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc
    16261 ggccaattgg taaaaccatt gttgcttgag tgtatatata ctcctcaaac acccttgatt
    16321 ctttgattta aatcaataaa aaccaca
//
LOCUS       DACADA010000102        15962 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.104, whole genome shotgun sequence.
ACCESSION   DACADA010000102 DACADA010000000
VERSION     DACADA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.104"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            184..786
                     /locus_tag="ISB17_003210"
     CDS             184..786
                     /locus_tag="ISB17_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP1981109.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(825..1250)
                     /gene="nudI"
                     /locus_tag="ISB17_003211"
     CDS             complement(825..1250)
                     /gene="nudI"
                     /locus_tag="ISB17_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAP1981110.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1529..2071
                     /locus_tag="ISB17_003212"
     CDS             1529..2071
                     /locus_tag="ISB17_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981111.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            2171..3373
                     /locus_tag="ISB17_003213"
     CDS             2171..3373
                     /locus_tag="ISB17_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAP1981112.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3593..4375
                     /locus_tag="ISB17_003214"
     CDS             3593..4375
                     /locus_tag="ISB17_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1981113.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4390..5595
                     /gene="rhmD"
                     /locus_tag="ISB17_003215"
     CDS             4390..5595
                     /gene="rhmD"
                     /locus_tag="ISB17_003215"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAP1981114.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5652..6941
                     /locus_tag="ISB17_003216"
     CDS             5652..6941
                     /locus_tag="ISB17_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981115.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6959..7762
                     /gene="yfaU"
                     /locus_tag="ISB17_003217"
     CDS             6959..7762
                     /gene="yfaU"
                     /locus_tag="ISB17_003217"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAP1981116.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(7803..8024)
                     /locus_tag="ISB17_003218"
     CDS             complement(7803..8024)
                     /locus_tag="ISB17_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981117.1"
                     /translation="MTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8037..9032)
                     /locus_tag="ISB17_003219"
     CDS             complement(8037..9032)
                     /locus_tag="ISB17_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAP1981118.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(9225..10415)
                     /gene="glpC"
                     /locus_tag="ISB17_003220"
     CDS             complement(9225..10415)
                     /gene="glpC"
                     /locus_tag="ISB17_003220"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAP1981119.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10412..11671)
                     /gene="glpB"
                     /locus_tag="ISB17_003221"
     CDS             complement(10412..11671)
                     /gene="glpB"
                     /locus_tag="ISB17_003221"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAP1981120.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11661..13289)
                     /gene="glpA"
                     /locus_tag="ISB17_003222"
     CDS             complement(11661..13289)
                     /gene="glpA"
                     /locus_tag="ISB17_003222"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAP1981121.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13562..14920
                     /gene="glpT"
                     /locus_tag="ISB17_003223"
     CDS             13562..14920
                     /gene="glpT"
                     /locus_tag="ISB17_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAP1981122.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14925..>15962
                     /gene="glpQ"
                     /locus_tag="ISB17_003224"
     CDS             14925..>15962
                     /gene="glpQ"
                     /locus_tag="ISB17_003224"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP1981123.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTD"
BASE COUNT         3692 a         3926 c         4268 g         4076 t
ORIGIN      
        1 ttagacgtaa atattgaaat ttttatattt tttcttattt agactttgca tttggcaaaa
       61 ttttgaggca ttttgccggc atcataggat ttttaatatt acaccaactg ctaattatcg
      121 ccagaaaagt cgctcaattt cacggtaatt gtctggttgc gcttgtctac aggtggagtt
      181 tacgtgttag ctttttgccg atcttcgttg aagtcaaaaa aatatttcat cattttactg
      241 gcgctcgctg caattgccgg actggggact catgccgcct ggagtagcaa tggtttgcca
      301 cgcatcgaca ataaaacact ggccagactg gcacagcagc acccggttgt cgttttgttt
      361 cgccatgctg aacgttgcga tcgttcaacc aatcaatgct tgtcagataa aacaggtatt
      421 acggtcaaag gtacccagga tgcccgtgaa ctgggcaacg cttttagtgc tgatatccct
      481 gattttgatc tctattccag taataccgtc cggactattc agtcggcgac ctggttttcg
      541 gcgggtaaaa agctgacggt agataaacga tttcttcaat gcggtaatga gatttatagt
      601 gcaattaaag acttacaacg caaagcacct gataaaaata tcgttatttt tacccataat
      661 cattgcctga catatattgc taaagataag cgtgatgcga catttaaacc tgattatctg
      721 gatggtttag tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga attcgttaac
      781 cactaaattt tcaatctgac agccagtgat ggctgtcatc attgttaaag aagacctttc
      841 aaacgtaacg tttttcgggt ggcaacattc aaatcataat gcaccagatc ttcaggtttt
      901 acccacgcgt agtcctgaaa ctcttcgttt attttcacat ctcggttggc agaaacgcag
      961 tcaaaaatca ggtaaatcat ataaatctct tccttgcgac catctgcata cgtcttggtg
     1021 cgaatatcat cgctgaaggt ccacggcgtg atttctgtca aaagcagctg ttctcccagt
     1081 tcttcgcgaa tttcgcggcg tagtgcctct tcaattcgtt cgccaggctc cacgccgcca
     1141 cccgaaagcg cccattgacc ggggaaaacg ccgcgatcgt cggccatttt acacagcaaa
     1201 taagcaccat cattttgaat caaagggcat acaatagtcc gttgtcgcac aggggtattc
     1261 cttataaata aaaagaaacc atgagaatac ggtaactcag aaatatgtga aagtgacagt
     1321 aatctctttt gcgatgataa ctacatttat tattattttt gtgtgtatcg ataaagtgtg
     1381 tttatttttt cggcgtttaa ttatttaaga agtatttagt tacaataagt gactagatcc
     1441 cttttttcgc tcattgaatc ctctgcgcca ggcagtacaa ttccgcagct ttgggaattt
     1501 tgattaattg cttgtgggaa agaggataat gaaaaaaatt gcgctagcag gtctggccgg
     1561 aatgctgttg gtttctgcat cggtcaatgc aatgagcatc agcggccagg cgggtaaaga
     1621 atacaccaat attggtgtcg gttttgggac tgaatcgacg ggcctggcgt taagcggtaa
     1681 ctggacacat aacgacgacg acggtgatgc cgcgggcgtg gggctggggt tgaatctgcc
     1741 tctcgggcca ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc cgaacgatgg
     1801 tgatgaaggt tatgcagcgg cagttggcgg tggtttgcag tggcaaattg gtgacagctt
     1861 ccgcttgttt ggtgagtatt actactctcc ggattcgctc tccagcggaa ttaaaagtta
     1921 tgaagaagcg aatgctggcg cgcgttacac cattatgcgt ccagtcagca ttgaggctgg
     1981 ctaccgttac ctgaacctgg caggtaaaaa tggcaatcgt gataacgcca ttgctgatgg
     2041 tccgtatgtt ggggtaaatg ccagcttctg acgccttccg gcgcggcgtt ctgtcgcgcc
     2101 attcagtttc ttactacgcc ctgtctccct ttgcgttata gtgattcgat aacaaaagca
     2161 ggagataacc atgttaaaag tggaaatgtt atccaccggg gatgaagtgt tacacgggca
     2221 aatcgttgac actaacgctg cctggctggc cgattttttc tttcatcagg ggctgccatt
     2281 atctcgccgc aatacggtgg gggataacct tgatgactta gtcaccattc ttcgcgaacg
     2341 tagtcagcac gccgatgtgt tgatcgttaa cggcgggctg ggaccgacca gtgatgattt
     2401 aagcgcactc gccgctgcga cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct
     2461 caaagagatg gaacgctatt tccatgaacg gggacgagta atggcaccga gcaaccgtaa
     2521 acaagcggag ctgcctgcca gtgctgaatt tatcaataac ccggtaggca ctgcctgtgg
     2581 ttttgccata cagcttaatc gttgcctgat gttctttacc cccggcgtac cgtcagaatt
     2641 taaggtgatg gtcgagcacg aaatcctgcc gcgcctgcgc gagcgttttt ctttaccaca
     2701 gccgccggtt tgcctgcgtt tgactacctt tggtcgttcg gaaagcgatc tggcacaaag
     2761 cctggacact ctacaactgc cgccgggcgt aacaatgggc tatcgctcct caatgccgat
     2821 tatcgaactg aaactcaccg gaccggcaag cgaggaacag gcgatggaaa aactgtggct
     2881 ggacgttaaa cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag gactgcccgc
     2941 gcagatcagt cgcgaattgc agagccgcca gttcagcctg acgctgagcg agcaattcac
     3001 cggtggttta ttggctttgc aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt
     3061 ggttccttca caggaggaaa ccctggcgca aactgcgcac tggattacag aacggcgggc
     3121 gaaccatttt gctgggttgg cactggctgt ttctggtctg gaagatgagc atctcaactt
     3181 tgcactagcc acgccagacg gcactttcgc tttgcgtgta catttcagtg ccacacgcta
     3241 cagtctggct attcgccagg aagtgtgcgc aatgatggcg cttaatatgc tacgccgttg
     3301 gttaaacggc caggacatcg ccagcgagca tggctggatt gaggttgttg agtccatgac
     3361 cttatctgtc tgattccgcc actctgtgcc ggtttactgc accggcacat tccccctccg
     3421 gcaaaatttg tccaacctgt tgtactcaat aagaaaaaat ccgtgcgctg attcacaaat
     3481 ttgtgcgtaa tttctccagt ccgctggacg agaaacggga tttgatgcgc ttcacaattt
     3541 tactccccct gaacaacgag actgattcca gtatctaaga ctggagtcca gtatgttgga
     3601 atcaagcaaa gttcccgccc tgacacgggc tattgatatt ctcaatttga ttgcccggat
     3661 tggcccctgt agtgctgcga cgatcattga cacattgggg atccctaaaa gcacagccta
     3721 tttgctgctt aatgaactca ggcgtcagcg ttttcttagc cttgatcacc aggaaaattt
     3781 ttgtttgtgg accaggctgg tggagctttc cggtcatgcg ttaagcaaga tggatctccg
     3841 ggaactggct cgaccgcgtt tgacgcaact gatggatacc accgggctgt tatgtcacct
     3901 ggggattatt gataacggca gcgcttatta cattttgaaa gtggagtcat cagccactat
     3961 cagcgtgcgt tcccatgaag gaaaaagcct ttcgctttat cgctccggaa taggtaaatg
     4021 cctgcttgcc tggcaacctg cagctgtaca gcaaagtatt atcgaagggc tggtctggga
     4081 acgggcgaca cccacaacga ttactcatcc acaacggctc tacgaagaac tggcgcgtat
     4141 tcgccgccag ggctggagct atgacaacgg cgaagattac gccgatgtcc gttgtgtcgc
     4201 tgcgccggtg tttaacgcca ataacgagct taccgctgcg atttcggtgg tcggtacccg
     4261 cttacaaatc aatgaagaat accgtgatta tctggcgggc aaggccattg cctgtgccag
     4321 ggatatttca cgtttgttgg gatggaaaag tccctttgac ttacaggcct cataacaatg
     4381 gagaacatca tgaccctacc aaaaatcaaa caggttcgcg cctggtttac tggcggtgcg
     4441 acagcagaaa aaggcgctgg cggcggtgat tatcacgacc agggggcgaa tcactggatt
     4501 gatgatcata ttgccacccc gatgagtaaa taccgcgaat acgagcagtc ccgccagtca
     4561 tttggcatta acgttcttgg cacgttgatt gttgaagttg aagcagaaaa cggccagacc
     4621 ggattcgctg tttcgacagc cggtgaaatg ggctgtttta ttgtcgaaaa acatcttaac
     4681 cgtttcattg agggtaaatg cgtcagtgat atcaaactga tccacgatca aatgctcaat
     4741 gccaccctgt attactccgg ctctggtggc ctggtgatga atacgatttc ctgtgtcgat
     4801 ctggctctgt gggatctgtt cggcaaagtg gtcgggcttc cggtttataa acttttaggc
     4861 ggcgctgttc gtgatgagat tcagttctac gccacaggtg cgcgtccgga tctggcaaaa
     4921 gagatgggct ttatcggtgg caaaatgccg acgcactggg ggccacatga tggcgatgcg
     4981 gggatccgca aagatgccgc tatggtcgcg gatatgcgtg aaaaatgcgg tgaggatttc
     5041 tggttaatgc tcgactgctg gatgagtcag gacgtgaatt atgccaccaa actggcccac
     5101 gcttgcgcgc cctataacct gaaatggatc gaagagtgcc tgccgccaca gcagtatgaa
     5161 ggttatcgcg aactgaaaca caacgcacca gcggggatga tggtcaccag cggtgagcac
     5221 cacggcacac tgcaatcttt ccgtacgctt tcagaaaccg gtatcgacat tatgcagccg
     5281 gatgttggct ggtgcggtgg cttaaccacg ctggtggaaa ttgccgcaat cgccaaatcc
     5341 agggggcaac tggtggtgcc acacggttcg tctgtttact cccaccatgc ggtgatcacc
     5401 ttcaccaata cgccattcag cgaattcctg atgaccagcc cggattgttc aacgatgcgt
     5461 ccgcaatttg atccgattct gcttaatgag ccagtaccgg tgaatggtcg tattcataaa
     5521 tcagtgcttg ataaacccgg tttcggcgtc gaactcaatc gtgactgcaa tttgaaacgc
     5581 ccctacagcc actaatcact ccgggcgttg ctgtgacaac ggtaacgccc acatattcta
     5641 aggagctttt tatgagcacc gctttgcttg acgccgtggt gaagaaaaac cgcgcgcgtt
     5701 taattccgtt tatgttggcg ctgtatgtgc tggcgtttct cgaccgttcg aatattggtt
     5761 ttgccaaaca gacctaccag attgataccg ggctgagtaa tgaagcttat gcgctgggag
     5821 caggcatttt ctttgtggta tacgcgtttc tgggggttcc ggcgaatctt ttgatgcgca
     5881 aactgggggc cagaacctgg attggtacga caacactgct gtggggattt ctttcggctg
     5941 ccatggcatg ggccgatact gaagcgaaat ttctgatcgt tcgcactctg cttggtgctg
     6001 cggaggccgg gtttttccct ggtatgattt atctcacttc gcaatggttt ccgcagcgta
     6061 atcgcgccag cattatgggg ctgttctata tgggcgcacc gctggcgtta acactgggat
     6121 caccgctttc cggcgcgctg ctggagatgc atggatttat ggggcatccc ggctggttct
     6181 ggatgtttgt gattgaagga ttgctggcag tcggcgctgg ggtattcaca ttcttttggc
     6241 ttgatgacac accggggcag gcacgttttc tgagtaaaca agaaaaaacg ttgcttatca
     6301 atcaactggc aagtgaagaa caacagaaag tgacttctcg attgagcgat gcgctgcgta
     6361 atgggcgagt ctggcaactg gcgattatct acctgaccat tcaagtggcg gtttacggat
     6421 taattttctt cctgccgacc caggttgctg cattgctggg gacaaaagtg ggctttacgg
     6481 cgtcggtggt caccgccatt ccgtgggttg cggccttgtt tgggacctgg cttattccgc
     6541 gctactccga taaaaccggc gaacggcgta atgtcgcagc gctgacatta ctggcggcgg
     6601 gcattggtat tggtctctcc gggctgcttt ctccagtact ggcgatcgta gcactgtgtg
     6661 ttgcagccat cgggtttatt gccgtgcaac cggtgttctg gacgatgccg acacaacttc
     6721 tttccggtac ggcgctggct gcggggattg gttttgtaaa cctgtttggt gcagtgggcg
     6781 ggtttattgc cccgatcctg cgcgtgaaag cagaaacgtt attttccagt gatgcggcgg
     6841 gattactgac gctggcagcg gtggcggtca tcggttcgct gattattttc actctgcgtg
     6901 taaatcgcac tgttgcgcag accgacgtgg cacatcatta aatagtttaa ggaacacgat
     6961 gaacgcatta ttaaccaatc cctttaaaga acgtttacgc aagggcgaag tgcaaattgg
     7021 tctgtggtta agctcaacga ctgcctatat ggcagaaatt gccgccactt ccggttatga
     7081 ctggttgctg attgacgggg agcatgcgcc aaacaccatt caggatcttt atcaccagct
     7141 acaggcggta gcgccctatg ccagccaacc cgtgatccgt ccggtggaag gcagtaagcc
     7201 gctgattaaa caagtcctgg atattggcgc gcaaactcta ctgatcccga tggtcgatac
     7261 tgccgatcag gcacgtcagg tggtgtctgc cacgcgctat cctccctacg gtgagcgtgg
     7321 tgttggggcc agtgtggcac gggctgcgcg atggggacgc attgagaatt acatggcgca
     7381 agtcaacgat tcgctttgtc tgttggtgca ggtggaaagt aaaacggcac tggataacct
     7441 ggacgaaatc ctcgatgtcg aagggattga tggcgtgttt attggacctg cggatctttc
     7501 tgcgtcgttg ggctacccgg ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac
     7561 cagcattcgg cggatccgtg ctgcgggtaa agcggctggt tttctggctg tggctcctga
     7621 tatggcgcag caatgcctgg cgtggggagc gaactttgtc gctgttggcg ttgacacgat
     7681 gctctacagc gatgccctgg atcaacgact ggcgatgttt aaatcaggca aaaatgggcc
     7741 acgcgtaaaa ggtagttatt gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt
     7801 aattagtggt tattcgctgc cagatcggct tcgcttagct gagtggccgc gagcacctga
     7861 tcacgatcaa tgccctgttc cagcatgcgc aaagcaattt taatggcttg ttcatgcatg
     7921 ccttcctgca aaccttctaa tttaccttct tgccagcctt caatcttacc ctcctgccag
     7981 ccaatttgat gtccttcctg ccgtagccgt tcagcaatag tcatcaatct ctccttttaa
     8041 tgacagttcg gaatatcagc atcagtgagt tgggttgccg ccagcacaat ctcacgttca
     8101 aagccctgct ccagcatgcg caaggcaatt ttaatggctt gctcatgcat gccttcctgc
     8161 aaaccttcta atttaccttt ctgcaaaccc tccaatttac cttgctgcaa accttctaat
     8221 ttaccttctt gccagccttc aattttacct tcttgccagc caatttgatg tccttcctgc
     8281 cgtagccgtt cagcaatagt cattaatctc tccttttgta gtggtgaacg ttcggcaatc
     8341 tcctgaataa agcgggtgaa acgggaggta tcgccgcatt gcagcagata attaaacagt
     8401 gtttgtagct ggctgtcatt agtgaagtct ctaaccaaaa gcgtggtgat cctgtcgacc
     8461 ataccgatta aatcgcggtc gcgaatatgc ttttgaatca gttccagcag agctatacgc
     8521 cgatgttgca tgatctcatc gtcaggtacg atggtgatat ccaccaacgg aaaagcttcg
     8581 gtgtacaact gccgggcaag ttgcggatcg tcaaactcat ccagccagtt gagtgagtac
     8641 gggtagggcg aggcttcgcc atgataaaac agcaatggca ccaccagcgg aactctgtca
     8701 tagcctttat ccaggtgacg ctgcatggcg gcagtggcat agcgcattag ccgaaaagcc
     8761 atattctttt ctgcagagct ttgatgttca atcacgcagt agatatagcc gtcaccctcg
     8821 ctggtttcca cggaccacaa aacatccgag tagtaagcgc gtaaactttt ttcaataaaa
     8881 ctggtgggtt ccaggcgtaa ggtttgcagg ttgcaaagct tgcgcagcgg ctctggtaga
     8941 tgtatttcca gaaaatcccg tgcggtttcg ggtgtgaaca taaaggtttt aaacaccgca
     9001 tcatgcgggg aggaggttgt tgattcggtc atggttgtcc gtcacctgat atttatgatg
     9061 acgcgacatt acggttatcc caggcctttg atatctgcca gtttcagcct ttgcgagacg
     9121 ttttcagagg aattttcact gcgttgcaac gcatacattg ggcacggaat cgtgtacgca
     9181 aaaatgcccg gtaacaacat gcttaccggg catcagaaag gagtttaagc caacgcctgt
     9241 gccagcagcg taatcggatg ttcgcagcga agacttgtgg acatctcaat ctgccatttg
     9301 caggtttcgc agtcggtgac caccagatct gcgccgctct cttctatctg gcggaacagt
     9361 ggtgcgccga tggcttgtga agtggggtag ttctcttttt tgaaaccgta agttcccgca
     9421 ataccgcagc actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc
     9481 tccagggtgt agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact
     9541 ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag
     9601 cgggtcgcca gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttctggg
     9661 tattcgtcgc gcagggcaaa tgtacacgtt gaggaggtgg caatcaccgg aatgcctttt
     9721 actcccacag cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg
     9781 gtaaaaccgt tggcgatcag cggtacgccg cagcattttt ctttactgag cagttgtacg
     9841 ccggtatcca tcgcgttgag cactttaatt aaatctttgc ctagttgcgg atggttgtag
     9901 ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat actgcgcttg ctgagccgcc
     9961 acgctgcgat accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcggcgatga
    10021 tcgattttta acgccgcatc aagcagctga cgcaccggtt tcagcgaggt agcggtgttg
    10081 acgatgggtg cgaacggcgt ggatacgcta cccatcaggt cggtatgact caacacaaaa
    10141 ttacgcagcg acgggcgcgt ggtgtcatat ttcgcccgcg cgcgctggat aatatcgcca
    10201 atcttcacat cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc
    10261 gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt
    10321 tttggccccg gataacccgg attcacccgg ctcaccgggc aggcggtggt gcagacggtg
    10381 cacttaatgc agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa
    10441 tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga
    10501 tgggatcaaa tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg
    10561 cctgcgacgg gcgtagtgcc tcatcagtgg ttacaccgaa ctgctgccac ggctgcggcg
    10621 caaaaaaatc gcccttatac cattcacccc gcgtagcggt ttgtagcaca tcaaggccga
    10681 gaatcggctc gcgaatgccg tcacgttccg ctaccagtcc gccgctaaag aaactgccgc
    10741 tcgcaagcac cgcaaatcgt gggcgcagcg ggatatcggc gtgattgcgc gtccagattt
    10801 cattcactac accattttta caggtcactt ttttcacttc atcgcccggc atccacacgc
    10861 cgccctggcg cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag
    10921 gcggcagcgt tggcaaaagc atcaatgaac aaggtagttt ttcattcaac caatgccaca
    10981 gtttgtcatc ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga
    11041 ccggaataag cgcatcaagt aacaacggcc agttttcttc attatcaagg aagcgggcaa
    11101 tattcaccgc ccggaactcg gtggcgttat tgcgcagcac atccagttcc ggcagttcaa
    11161 tttcagcggt ttcaacttta agatccaatt cgcgcaatga agccgccgca agatgcgcct
    11221 gaaaatccat caggccgcta attcccacta cacatatttt cttcgcgggc agcggccaga
    11281 cagggacttc tggcgaactt agccaggttg agcgcagagt gccgagcggc gtaatccgct
    11341 ggtgagccag ttctacgctg ccctgcaatt gcgcaccgct ttcagcgatt aatgcctgcg
    11401 cctggcaagc gagatcgagc acgcgttgag gcccgagaag agtgtaagga tgggcaggtg
    11461 cctgctgacg caaagattcc agtccactgt gaatgtctgt caccggttga ccatccggca
    11521 gatggctcag caaatccagc gatccagatg agaaatggag cgcgctttga ccacgagtga
    11581 caatggcaca gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc
    11641 cgccgcccat aatgacagta tcaaagcgca tctttctgct ccttctccag accacacaat
    11701 ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg catctcccca ggcgatgggt
    11761 tgcacgcctt tccagcgttc gtttaggaag gtggaaagtt gctcaataga ttgcgcggac
    11821 gtcgtgacgt taaaacgttg cagcagtccg gcagcgcggc aggcgcagag ctcgccctgg
    11881 caggtgccca tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa
    11941 ttttctaccg cgtactgcac ttcacccgca gttaccgctt cgcactcaca taccaggcta
    12001 cggtgcagac ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca
    12061 gaaccgcgca gcggggcagg tagggagatg actttacgca aggtaacttc agcgggttcc
    12121 tgtgaaccag gaagtgccag atcggcggtc gtacaggggc gcgtgttgcc cagtttgcgg
    12181 catacggcat cggtagccca ttccgccatc agccgatagg tcatcagttt gccaccggtg
    12241 atggtgataa acccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg
    12301 ctgacgttac gtccgctcgg atcgtcatcg ctggcaacca gcgggcgtac gccagagtag
    12361 gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga
    12421 atatcaactt cttctgccgt tactcgatta tcgtcaatct cgttgtaatc aatacgtaaa
    12481 gaggtggtgc caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt
    12541 ttgcggcagc ggttgatcac atgctgatta atgcggtgat ccatgatcag cagcgatcct
    12601 ttcgccggga acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc
    12661 ccggcggcat taactacgac aggtgcatga agtgcctgaa tttcgccggt gagatggtta
    12721 cgtacacgaa cgccgcacac cgccgcgcct tcacgaatca gccccgtgac ttcatgagcg
    12781 gtaaggataa cggcaccgtg ttctttggca tccagcatgt ttgctgctgt cagacgaaat
    12841 ggatcaacgg tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt
    12901 tcgataatgc gcgcttgctg cggatcaatg gcttctgcgc tgatccctgc ttcttcgcag
    12961 gcgcggataa aagtggcctg gaaggagagg tcatcttccg gcagggtgat aaacaggccg
    13021 ttggttggtt ctacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatacat
    13081 tcgcgggccg attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg
    13141 ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg
    13201 cgcagggcac agtcgcgggc aatcccggct cccgttgcgc caccgccaat gataatcacg
    13261 tcacttgatt gcgagtcgcg agttttcatt gttatccctc agaagttcgt tttttaccat
    13321 ttagccatag taaaaacatg aattgtttga tttcgcgcat attcgctcat aattcgaaag
    13381 tgaaacgtga tttcatgcgt cattctgaac atttcaacca tcgtatttaa taatgtgtgc
    13441 ggtaattcac atttaattta tgaatgtttt cttaacatcg cggcaactca agaaacggca
    13501 ggttctccca ctgaatcagg ctgttaatca taaataagac cacgggccac ggaggctatc
    13561 aatgttgagt atttttaaac cagcgccaca caaagcgcgc ttacctgccg cggagatcga
    13621 tccgacttat cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgctgc
    13681 ttactatttg gttcgtaaga actttgcgct cgctatgcct tatctggttg agcagggatt
    13741 ctcacgcggt gatttaggtt ttgccctttc ggggatctcg attgcttatg gattttcgaa
    13801 attcatcatg ggttcggtat cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt
    13861 gattctggcg gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat
    13921 tgcggtgatg tttgtactgt tgttcctctg cggttggttc caggggatgg ggtggccgcc
    13981 gtgtggtcgt actatggtgc actggtggtc gcagaaagaa cgtggcggta ttgtgtcagt
    14041 gtggaactgt gcgcacaacg tcggtggtgg tattccgccg ctgctgttcc tgctggggat
    14101 ggcctggttc aatgactggc acgcggcgct ctatatgccc gctttctgcg ccattctggt
    14161 ggcactgttc gcctttgcga tgatgcgcga taccccgcaa tcctgtggcc tgccgccgat
    14221 tgaagagtac aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac
    14281 cgcgaagcag atcttcatgc agtacgtgct gccgaacaaa ctgctgtggt atatcgccat
    14341 cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttatct
    14401 gaaagaggtt aagcatttcg cgctggataa atcctcctgg gcctactttc tttatgaata
    14461 tgcaggtatt ccgggcaccc tgctgtgcgg ctggatgtcg gataaagtct ttcgtggtaa
    14521 ccgtggggca accggcgtct tctttatgac actggtgacc atcgcgacta tcgtttactg
    14581 gatgaacccg gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct
    14641 gatctatggc cctgtgatgc tgatcggtct acatgcgctg gaactggcac cgaaaaaagc
    14701 ggcaggtacg gcagcgggtt ttaccgggct gtttggttac ctgggcggtt cggtggcggc
    14761 gagcgcgatt gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat
    14821 gattggcggc agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg
    14881 tcgccatgaa caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctga
    14941 aaaaccttag catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg
    15001 cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc
    15061 atacgctgcc agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt
    15121 tggtgatgac caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta
    15181 ctgatgttgc cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag
    15241 atttcacgct ggatgaaatt aagtcgctga aatttaccga aggtttcgat attgaaaacg
    15301 gtaaaaaagt gcagacttat ccggggcgtt tcccaatggg taaatctgac ttccgggtgc
    15361 acacctttga agaagagatt gaatttgttc aggggttaaa ccactctacc gggaaaaata
    15421 tcggtatcta tccagaaatt aaagcgccgt ggttccatca tcaggaaggg aaggatattg
    15481 cggcaaaaac gctggaagtg ctgaagaaat atggttacac cggtaaggac gacaaagttt
    15541 atttgcaatg ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacctaaaa
    15601 tgggtatgga tctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc
    15661 agaaacagcc ggacggaagc tgggttaatt acaattacga ctggatgttt aagccgggtg
    15721 ccatgaaaca ggtggcggaa tatgctgatg gtattggtcc ggattaccat atgttgattg
    15781 aggagacatc gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga
    15841 ataaactggt agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactactg
    15901 atgtgaatca gttatatgat gttctgtata acaaagcggg tgtaaatggg ctatttactg
    15961 ac
//
LOCUS       DACADA010000103        15840 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.105, whole genome shotgun sequence.
ACCESSION   DACADA010000103 DACADA010000000
VERSION     DACADA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.105"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(201..644)
                     /gene="mioC"
                     /locus_tag="ISB17_003225"
     CDS             complement(201..644)
                     /gene="mioC"
                     /locus_tag="ISB17_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAP1981124.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHNIPEDPAEEWLGSWINLLK"
     gene            complement(734..1192)
                     /gene="asnC"
                     /locus_tag="ISB17_003226"
     CDS             complement(734..1192)
                     /gene="asnC"
                     /locus_tag="ISB17_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAP1981125.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            1344..2336
                     /gene="asnA"
                     /locus_tag="ISB17_003227"
     CDS             1344..2336
                     /gene="asnA"
                     /locus_tag="ISB17_003227"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAP1981126.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSKEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(2341..3792)
                     /gene="viaA"
                     /locus_tag="ISB17_003228"
     CDS             complement(2341..3792)
                     /gene="viaA"
                     /locus_tag="ISB17_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAP1981127.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3786..5282)
                     /gene="ravA"
                     /locus_tag="ISB17_003229"
     CDS             complement(3786..5282)
                     /gene="ravA"
                     /locus_tag="ISB17_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAP1981128.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            5505..7373
                     /gene="kup"
                     /locus_tag="ISB17_003230"
     CDS             5505..7373
                     /gene="kup"
                     /locus_tag="ISB17_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAP1981129.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            7540..7959
                     /gene="rbsD"
                     /locus_tag="ISB17_003231"
     CDS             7540..7959
                     /gene="rbsD"
                     /locus_tag="ISB17_003231"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP1981130.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            7967..9472
                     /gene="rbsA"
                     /locus_tag="ISB17_003232"
     CDS             7967..9472
                     /gene="rbsA"
                     /locus_tag="ISB17_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAP1981131.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            9477..10442
                     /gene="rbsC"
                     /locus_tag="ISB17_003233"
     CDS             9477..10442
                     /gene="rbsC"
                     /locus_tag="ISB17_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAP1981132.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            10467..11357
                     /gene="rbsB"
                     /locus_tag="ISB17_003234"
     CDS             10467..11357
                     /gene="rbsB"
                     /locus_tag="ISB17_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HAP1981133.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            11483..12412
                     /gene="rbsK"
                     /locus_tag="ISB17_003235"
     CDS             11483..12412
                     /gene="rbsK"
                     /locus_tag="ISB17_003235"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1981134.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            12416..13408
                     /gene="rbsR"
                     /locus_tag="ISB17_003236"
     CDS             12416..13408
                     /gene="rbsR"
                     /locus_tag="ISB17_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAP1981135.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(13374..14801)
                     /locus_tag="ISB17_003237"
     CDS             complement(13374..14801)
                     /locus_tag="ISB17_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP1981136.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(14824..15516)
                     /locus_tag="ISB17_003238"
     CDS             complement(14824..15516)
                     /locus_tag="ISB17_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1981137.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         3839 a         3884 c         4151 g         3966 t
ORIGIN      
        1 aggatccggc ttttaagatc aacaacctgg aaaggatcat taactgtgaa tgatcggtga
       61 tcctggaccg tataagctgg gatcagaatg aggggttata cacaactcaa aaactgaaca
      121 atcgttgttc tttggataac taccgtttga tccaagcttc ctgacagagt tatccacagt
      181 agatcgcacg atctgtatac ttatttgagt aaattaatcc acgatcccag ccattcttct
      241 gccggatcct ccggaatgtt gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt
      301 tttgctccgg aatttttgag ttctgcctcg attttatcga tagccccaca aaaggtgtca
      361 tattcacgac taccaatacc gattgcgcca aagcggaccg cagaaagatc gggcttctgt
      421 tcctgcaatg cttcatagaa aggagaaagg ttgtccggaa tatctccggc accgtgggtt
      481 gagctgataa ccagccagat ccccgaggca gataaatctt ccaacagcgg accgtgcagc
      541 gtttcggtgg taaaacccgc ctcttccagc ttttcagcca ggtgttctgc tacatattcg
      601 gcaccgccga gggtgctgcc gctgataaga gtgatatctg ccataaaccg ccacctttat
      661 taagagtggc gtattgtacg ctgtgaacgc gttgggatct acctgtggaa aagtatggga
      721 ttaaaaaagc cgatcagggc ttaatggtac gcatgatcgg gttctgcagg acgatcagtg
      781 tctcggtgga ctgaatttca tcaattgttt ggatcttgtt gataagtaca tgctggagag
      841 catcgatcga acggcacatc acttttataa agatgctgta gtggccggtt gtgtaatagg
      901 cttcagtgac ttcatcgagg ctttccagct ttgccagcgc ggaaggatag tctttggcgc
      961 tctttaatat aatgccgata aagcagccta cgtcataacc aagctgcttc gggctgacat
     1021 caatacgcgc cccggtaatg atccccgcct gcttcatttt ctctactcga acgtgaatcg
     1081 tccccggact gacgccaaat tgtttcgcca gttcggcgta agcggtgcgc gcattgccca
     1141 ttaatgcttc caggatgcca cggtccagat tgtcgatcag ataattttcc ataggatttt
     1201 cttatgcgga ttgatgattc attctatttt agccttcttt tttaatgaat caaaagcacg
     1261 tgaggctttt tattgaatga ttattgcatg tgtgtcggtt tttgttgctt aatcataagc
     1321 aacaggacgc aggagtataa aaaatgaaaa ccgcttacat tgccaaacaa cgtcaaatta
     1381 gcttcgtgaa atctcacttt tctcgtcaac tggaagaacg tctggggctg atcgaagtcc
     1441 aggcgccgat tcttagccgt gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa
     1501 aagcggtgca ggtaaaagtg aaagctctgc ctgatgccca gttcgaagtg gttcattcac
     1561 tggcgaagtg gaaacgtcag accttagggc aacacgactt cagcgcgggc gaagggctgt
     1621 acacgcacat gaaagccctt cgccccgatg aagaccgtct ttccccgttg cactcggtct
     1681 atgtagacca gtgggactgg gaacgcgtaa tgggcgacgg cgagcgtcaa ttctcgactc
     1741 tgaaaagcac ggtagaggcg atctgggcgg gaattaaagc aaccgaagct gcggttagca
     1801 aagagtttgg cctggcaccg ttcctgccgg atcagatcca cttcgtacac agccaggagt
     1861 tactgtctcg ttatccggat ctcgatgcca aagggcgtga gcgggcgatt gcgaaagatc
     1921 ttggcgcggt attcctcgtc gggattggcg gcaagttgag tgatggtcat cgtcacgacg
     1981 tgcgcgcacc ggattatgat gactggagca ccccgtcaga gctgggccat gcgggtctga
     2041 acggcgatat tctggtgtgg aacccggtac tggaagatgc gtttgagctt tcttccatgg
     2101 ggatccgcgt ggatgccgac acgctgaagc atcagctggc gctgaccggt gacgaagatc
     2161 gcctggagct ggagtggcat caggcgctgc tgcgcggtga aatgccgcag accatcggcg
     2221 gcggtatcgg gcagtctcgt ttgaccatgc tgctgctgca actgccacat atcggccagg
     2281 ttcagtgtgg tgtatggccg gcagctgttc gcgagagcgt cccttctctg ctgtaataat
     2341 ttatcgccgc cagcgtctga gcaggcggct tcgcatcccg gtatcaaagc gccagatatg
     2401 atcgaaaatg cgcatgatgc cgggtttgcc atgtgcagac atcgccacgg catgaaagcg
     2461 atgctgatgt acccgctgta gctctttcac tttactcgtc acgtcgtcag gcaaccgctg
     2521 ggcgataaaa tcagaaatca ccaccgcatc ggcatcaaac cattcccggc tttgcaagcg
     2581 ttccataatg gcgcgaaaac aactggcaag atcggtgccg ccacgaaact gctgacttaa
     2641 aaaacggatt gcttgttcga tgccttgtgg gcctgaaagc tcataacgga cgatctcggt
     2701 ggaaaatagc ataatatagc agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag
     2761 gcagaacgct ttcgcacact gttcattaaa gccgcccatt gagccggaag tatccacaca
     2821 gacaataaac ggtccgcgcg gctgttcgtc gtaatcttta tgcaccaccg ggcgttcgat
     2881 cactttttca cgccacgact caccatgcag gcgataggtg agcaactgtt tttccaccag
     2941 ccgacggtaa aactcatact ccagttctgt tatccctagt gtcgccagtt ctggcggcag
     3001 gagacgtaaa atatcatcgc tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg
     3061 ttcgcgcacc agggtgcgga aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc
     3121 ttcccgggaa cgccccagct gttctgccag gcgtttcagt tccggctgtt cgttaagaaa
     3181 ttcaccgtat ttcacaatca actgatagtc gccacgttta agctgaccgg cgctcatatc
     3241 ccacagacga ccagctgcgg tattgttatc tgcgagaatc ggttcaagtt gtccgctcag
     3301 cgtcatgcgt tcctgaactt cactcaacag ttgttcgcgt tcttcttcta atagctgttg
     3361 atttaacgtc gttgcttgca cgatcagact taaacgccaa cgctggagaa aaagcgtgtg
     3421 taacgctgaa gtgatcgtgc tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg
     3481 ggaattcaga cgatgcagta agtccaggat ctgtggtagc tgcacaataa actgcggcgt
     3541 ggagaggagc tggctttgct gatagcacat cacctcttcg gtgagttccg gcgggactcg
     3601 ggcatctttc agccgactgc gcagcgcctc acgccagcgg ggaacatcat cagtgattgc
     3661 cgccttcagt cgtgggaatt tttcaaagaa gactgccagc tgcggtgagg ccagcagcgc
     3721 gatgatcatc tcttcgatca atccctcttc gctgacggcc agcatcacat taagcgtatc
     3781 cagcgttagc attgttgtgc ctggcgaatc tgtgcgccga catcctgtag gctggcttca
     3841 atgcgaccta accagtcgcc agggataaac agacattttt gctgttcgct gaataaagcg
     3901 tgttgcttgc gccagtcgct ttcaagctct tccagttgtt gttttatttc acctggcaga
     3961 ccttcagccg atgaaccggg aagtgccagc gtactgcctt gtaaacttac atcgcgtaca
     4021 acaagatgtt gggcgctatc cacttccaga ttcagtttct gggcaaagcc gataccgttc
     4081 agtttgccgc gaatctcacc acctttactc agccactgtt ccagcgcgct acgctcaaag
     4141 gagatatgaa ccacttccat atcatgcaat tttaacggtt tttgcagcag cagagtcaga
     4201 gtggaagcag taacgttaac aggaagttga tactgctgac gacggctgaa aatgccgccc
     4261 agacgaatta ccgttaaggc tgttttatcg ctttgttgct gctggagttg caggtgacgt
     4321 tgcacaatcg cgcccaggcg ggtcaacatc ccttgctgtt gccaggcatg accggtcatc
     4381 aatacatcaa tttgttgttg tatcaaattc aggctttgcg cgtcatacca caggcaatct
     4441 ttcagcaaaa tgagatcaac cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc
     4501 tgcaataagc ggatcgcttt tttccagcga cgatccgaga cataaggcgc atccggtaat
     4561 ttatccagtt gctggcgcag cataaaaatc agctcaaata catgatcggg cagcgtaatt
     4621 tcaccaatct ctttctgcca gcgttcatat tcttcatctg tgatctgcaa ggaggcagga
     4681 accggattgt cattttcatc ctgttgactg gtcagcatgg agcggaaatt cgctttatcc
     4741 tgcactttat ctaaccacag acgaatcagc atgcggtcat ataacgcttc cagactgctg
     4801 tctgcttccg gcagctcgtt ggaggccgcc accagcagac gcatcgggat tttttctacg
     4861 agtgcgccgt tgcggaactg gcgctcgtta atggcggtga gcaaggtatt aagaattgcc
     4921 ggacccgctt tccagatctc atccagaaag acgatttcag cttccggcag gtaaccgctg
     4981 gttaaacgtt catagcgccc ttcatctttt agcgcctgaa tagaaagggg accaaaaact
     5041 tcttccggcg tggagaagcg ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg
     5101 aattttaagc gccgggcgat caaacttttg gcaatacctg gtgggccaag gaggaacaca
     5161 ctttcaccac ttaatgccgc taataaacac aagcggatgg cgtggctacg ttcataaagc
     5221 cccttttcca acgaactgct caggcgggaa attctttccg ctaataaatg agggtgagcc
     5281 ataatgaagt ggcgtccttt catcaaaagt tctgcgtaaa ttgcgagtat agacgtttct
     5341 tgctggtggc taaaatagtc tcaaaggagg ggtatttttc tttgagccag gttaatgtgg
     5401 ccgcatttag gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc
     5461 aagggactaa gcacacattt catatttcaa cgaaagacta gtctatgagc actgataata
     5521 agcaatcatt gcccgcgatt accctcgcgg cgattggagt tgtctacggc gatattggta
     5581 ccagcccgtt atatacactt cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac
     5641 gcgatgccgt gtttggcttt ttatcgctga tcttctggct gctaatcttt gtggtttcca
     5701 ttaaatatct caccttcgtg atgcgggcag ataacgccgg tgaagggggg atcctgacgt
     5761 tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg
     5821 ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat tactcccgct atttcggtga
     5881 tgtccgccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg atagttcccc
     5941 tctcaattat cgttctcaca ttattattca tgattcaaaa acatggcaca gctatggtcg
     6001 gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcaggg ctggggttac
     6061 gtagcattat tgctaacccg gaagtgctgc atgcactgaa tccgatgtgg gcggtgcatt
     6121 tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg ctgtcgatta
     6181 cgggggtcga ggcgctgtat gctgatatgg ggcactttgg taagttccct attcgtctgg
     6241 cgtggttcac cgtcgtattg ccttccttaa cccttaatta cttcggccag ggagcgctgt
     6301 tgttaaagaa tccggaagcg attaagaacc cgttcttcct gttggcaccg gactgggcgc
     6361 tgatcccgct gctgatcatc gccgcactgg cgacggtaat tgcctcgcag gcggttatct
     6421 ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca
     6481 ttattcacac ctccgaaatg gagtcagggc aaatctatat tccctttgtg aactggatgc
     6541 tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac ctggcggcgg
     6601 cgtacgggat tgcggtgacc ggaaccatgg tgctgacgtc tattctctcg actaccgtgg
     6661 cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt gctttccttt
     6721 gtgtcgatat tcccttgttc accgctaacc tcgataaact gctctccggt ggctggttgc
     6781 cattgagcct cggtacggtg atgtttatcg tgatgaccac ctggaagagc gagcgtttcc
     6841 gcttgctgcg gcggatgcat gaacatggta actctctgga agcgatgatt gcttcgctgg
     6901 agaaatcacc gcccgttcgc gtgcctggga ccgcggtgta tatgtcgcgt gcaatcaacg
     6961 tcattccctt tgcgctgatg cataacctta aacataacaa ggtattgcat gagcgggtga
     7021 ttctgttaac tctgcgcacc gaagacgcgc catatgtcca taacgtccgt cgggtacaga
     7081 ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc
     7141 caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagctgc cggatgatgg
     7201 aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg tggtatttgc
     7261 gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgggca ccggatcaat
     7321 ttgaaatccc gccaaacaga gttatcgaac tgggtactca ggtcgaaatc taacgccaga
     7381 cgcctccttt ctccataagg gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt
     7441 ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag tcaggattaa
     7501 actgcgggtc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt
     7561 aattctgata tttcatcggt gatctcccga ctgggacata ccgatacgct ggtggtgtgt
     7621 gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccagggt
     7681 gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgtc
     7741 attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac
     7801 cttgagcagc tgcaacaaca ccagggaaac accattgaaa ttcgttacac cacgcatgaa
     7861 caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga atgttctccg
     7921 tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg aagcattact
     7981 tcagcttaaa ggcatcgata aagcctttcc gggcgtaaaa gccctctcgg gcgcagcgtt
     8041 aaatgtctat ccgggccgcg tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac
     8101 catgatgaaa gtgcttactg gtatctatac tcgcgatgcc ggtacgcttt tatggttggg
     8161 gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg ggattatcca
     8221 ccaggaactg aacctgatcc cgcagttaac cattgccgaa aacattttcc tcggtcgtga
     8281 gtttgttaat cgctttggca aaattgactg gaaaaccatg tatgccgaag cggataaatt
     8341 gctggctaaa cttaacctgc gctttaaaag cgacaaactg gtgggtgatc tttccatcgg
     8401 tgaccagcaa atggttgaaa tcgccaaagt gctgagcttt gagtcgaaag tcatcattat
     8461 ggatgaaccg accgatgcgc tgaccgatac cgaaaccgaa tccttgttcc gcgtcatccg
     8521 cgagctgaaa ttgcaaggcc gcggtattgt ctatatctcc caccgcatga aagaaatctt
     8581 cgagatttgc gatgacgtta ccgtttttcg tgacggacaa tttattgctg agcgtgaagt
     8641 ggcatcactg accgaagatt ctctgattga gatgatggtg ggccgcaagc tggaagatca
     8701 atatccgcac ctggacaaag cgccgggaga tatccgcctg aaagtcgata atctctgcgg
     8761 acctggcgtt aacgatgtct cttttacttt acgcaaaggc gaaattcttg gcgtctctgg
     8821 tttgatgggc gctgggcgta ccgaactgat gaaagtgctc tacggcgcac tgccgcgcac
     8881 aagcggttac gtcaccctgg atgggcatga agtcgttacc cgttcgccgc aggatggcct
     8941 ggcaaacggc attgtttata tctccgaaga ccgtaaacgt gacggtttag tgctgggcat
     9001 gtcagtaaaa gagaacatgt cgctgaccgc gctgcgctac ttcagccgcg ctggcggcag
     9061 tttgaagcat gccgatgaac agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa
     9121 aacgccatcg atggaacagg caattggtct gctttccggt ggcaatcagc aaaaagtagc
     9181 gattgcccgt ggtctgatga cacgccctaa agtgttgatc ctcgatgagc ctacccgtgg
     9241 cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg
     9301 cttgagcatc attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat
     9361 catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccaaga
     9421 agtgttaatg gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga
     9481 caacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat
     9541 cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca
     9601 ccatcaataa cttattcaat attcttcagc aaacctcggt gaacgccatt atggcggtcg
     9661 ggatgacgct ggtgatcctg acgtcgggta tcgacttgtc ggtaggttct ctgttggcgc
     9721 tgaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg
     9781 ctgccgctct cgcgttaggt gccgcaattg gtgcggtaac tggggtgatt gtagcgaaag
     9841 gtcgcgtcca ggcgtttatc gctacgctgg ttatgatgct tttactgcgc ggcgtgacca
     9901 tggtttatac caatggtagc ccagtgaata ccggctttac tgagaacgcc gatctgtttg
     9961 gctggtttgg tattggtcgt ccgctgggcg taccgacgcc agtctggatc atggggattg
    10021 tcttcctcgc ggcctggtac atgctgcatc acacgcgtct ggggcgttac atctacgcgc
    10081 tgggcggcaa cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca
    10141 tcgtctattc tctttgtggt ctgctggcat cgctggccgg gatcattgaa gtggcgcgtc
    10201 tctcctccgc acaacccacg gcggggactg gctatgagct ggatgctatt gctgcggtgg
    10261 ttctgggcgg tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg
    10321 cattaattct tggcttcctt aataatggat tgaatttgtt aggtgtttcc tcctattacc
    10381 agatgatcgt caaagcggtg gtgattttgc tggcggtgct ggtagacaac aaaaagcagt
    10441 aataacgact acaggacatc ttgaatatga acatgaaaaa actggctacc ctggtttccg
    10501 ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg
    10561 tggtctccac gcttaacaac ccgttctttg tatcgctgaa agatggcgcg cagaaagagg
    10621 cggataaact tggctataac ctggtggtgc tggactccca gaacaacccg gcgaaagagc
    10681 tggcgaacgt gcaggactta accgtccgcg gcaccaaaat tctgctgatt aacccgaccg
    10741 actccgacgc agtaggtaat gctgtgaaga tggctaacca ggcgaacatc ccggttatca
    10801 ctcttgaccg ccaggcaacg aaaggtgaag tggtgagcca catcgcatct gataacgtac
    10861 tgggcggcaa aatcgccggt gattacatcg cgaagaaagc gggtgaaggt gccaaagtta
    10921 tcgagctgca aggcattgcc ggtacatccg cagcccgtga acgtggcgaa ggtttccagc
    10981 aggccgttgc tgctcacaag tttaatgttc ttgccagcca gcctgcagat tttgatcgca
    11041 ccaaaggctt gaacgtaatg cagaacctgt tgaccgctca tccggatgtt caggctgtat
    11101 tcgcgcagaa tgatgaaatg gcgcttggcg cgctgcgcgc actgcaaact gccggtaaat
    11161 cggatgtgat ggtcgtcgga tttgacggta caccggatgg cgaaaaagcg gtgaatgatg
    11221 gcaaactagc agcgactatc gctcagctac ccgatcagat tggcgcgaaa ggcgtcgaaa
    11281 ccgcagataa agtgctgaaa ggagagaaag ttcaggctaa gtatccggtt gatctgaaac
    11341 tggttgttaa gcagtagttt taatcaggtt gtatgacctg atggtgacat aaatacgtca
    11401 tcgacagatg aacgtgtaat ataaagaaaa gcagggcacg cgccacccta acacggtggc
    11461 gcattttatg gacatcccga atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat
    11521 taatgctgac cacattctta atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg
    11581 taaccactat caggttgcat ttggcggcaa aggcgcgaat caggcggtgg ctgctgggcg
    11641 tagcggtgcg aatatcgcgt ttattgcctg cactggcgat gacagcattg gtgagagcgt
    11701 tcgccagcag ctcgccactg ataatatcga tatttctccg gtcagcgtga tcaaaggcga
    11761 atcaacaggt gtggcgctga tttttgttaa tggcgaaggt gagaatgtca tcggtattca
    11821 tgccggcgct aatgctgccc tttccccggc gctggtggaa gcgcaacgtg agcgtattgc
    11881 caacgcgtcg gcattattaa tgcagctgga atcaccactc gaaagtgtga tggcagcggc
    11941 gaaaatcgcc catcaaaata agactatcgt tgcgcttaac ccggctccgg ctcgcgaact
    12001 tcctgacgaa cttctggcgc tggtggacat tattacgcca aacgaaacgg aagcagaaaa
    12061 gctcaccggt attcgtgttg aaaatgatga agatgcagcg aaggcggcgc aggtactgca
    12121 tgaaaaaggt atccgtactg tactgattac tttaggaagt cgtggtgtat gggctagcgt
    12181 gaatggtgaa ggtcagcgcg ttcctggatt ccgggtgcag gctgtcgata ccattgctgc
    12241 cggagatacc tttaacggcg cgttaatcac ggcgttgctg gaagaaaaaa cattgccaga
    12301 ggcgattcgt tttgcccatg cagccgctgc gattgccgta acacgtaaag gcgcacaacc
    12361 ttccgtaccg tggcgtgaag agatcgacgc atttttagac aggcagaggt gacgcttggc
    12421 tacaatgaaa gatgttgccc gcctggcggg cgtttctacc tcaacagttt ctcacgttat
    12481 caataaagat cgcttcgtca gtgaagcgat tacagccaaa gttgaagcgg cgattaaaga
    12541 actcaattac gcgccatcag ctctggcgcg tagcctcaaa ctcaatcaaa cacataccat
    12601 tggcatgttg atcactgcca gtaccaatcc tttctattca gaactggtgc gtggcgttga
    12661 acgcagctgc ttcgaacgcg gttatagtct tgtcctttgc aataccgaag gcgatgaaca
    12721 gcggatgaat cgtaatctgg aaacgctgat gcaaaaacgc gttgatggct tgctgttact
    12781 gtgcaccgaa acgcatcagc cttcgcgtga aatcatgcaa cgttatccga cagtgcctac
    12841 cgtgatgatg gactgggctc cgttcgatgg cgacagcgat cttattcagg ataactcgtt
    12901 gctgggcgga gacttagcaa cgcaatatct gatcgataaa ggtcataccc gtattgcctg
    12961 tattaccggt ccgctggata aaactccggc gcgcctgcgg ttggaaggtt atcgggctgc
    13021 gatgaaacgt gcgggtctca acattcctga tggctatgaa gtcactggtg attttgaatt
    13081 taacggcggg tttgacgcta tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt
    13141 ctttaccgga aatgacgcta tggctgttgg cgtttaccag gcgttatatc aggcagagtt
    13201 acaggttccg caggatatcg cggtgattgg ctatgacgat atcgaactgg caagctttat
    13261 gacgccacca ttaacaacta tacaccaacc gaaagatgaa ctgggggagc tggcgattga
    13321 tgtactcatc catcggataa cccagccgac cctacagcaa caacgattac aacttactcc
    13381 gattctgatg gaacgcggtt cggcttagat ttacgctgtc ttttgatcaa attattacca
    13441 tcggttgttt tcagtagcat gaacattgct gctgaagcaa cagtaataat gcccatcgtg
    13501 ataaacgtat agtggaattg ttcgactgtc gttgtgcctt ccatcccttc gtaaacgcga
    13561 aggacggccg cacttacagc aacgcctaaa ctgatagaca gttgctgcgt gaccgccaga
    13621 acactgttac cgctgctggc gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc
    13681 gcggtaaatt gcgtcgacat agccatccct aatataaaca acggcaagat cagcatccat
    13741 atcgccattg ctggtgattg caaagagaac tgagcgatca ttagcccaat aatcaccgtg
    13801 atccccacta acgtatggcg atagcccaga cgacgtaaga cttgggtaac catcgatttt
    13861 gcaataatgg aacctaacgc tgtcggtgcc atcatacagc cagcaataaa cgcctgatac
    13921 ccaaatccta cctgtaacat caatggcata aggaacggta cacagccggt ccccagacgg
    13981 gttgcaatat tgcctacgat accgatcgag aaagtgcggg ttttaaataa atctaatgaa
    14041 attaatgggt ttggcgtgcg tcgcgcatgg agaatataga gaagcagtaa ccagatgctg
    14101 gtgacaatta ccgtcaaggc aatccagctg gcgacaatct tttccccgaa tagctctatt
    14161 ccgcttgaga agagaacaag gctgaggcca aacagcaaaa agccagtggt atcgaatctg
    14221 cgtcgtgcgg tggtgaaatt gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct
    14281 atggggatat tgattaaaaa tatccagtgc caggttgccc aggtgaccag cacgccgcca
    14341 agaacggggc ctaaaattgg ccccaccaga cccggcatgg cgacaaaatt caatacagga
    14401 agaagttcat tacgaggata agcgcgcagt aaggccagcc gagcaacagg catcatcatt
    14461 gcgccgccta tcccctgaat aacccggaag acaaccagct gtggtagcga gttggaaagt
    14521 gcgcaggcca gagaacccaa cgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta
    14581 ccgaagcgat cggctagcca tccgcttacc ggaataagca tcgccaccgt cagcgtataa
    14641 ctgatgatgg ctgattgcat cgcgagagga gaacgattaa ggctatgagc gattgcgggt
    14701 aaggcggtat tcagaatagt ggcatcaagt gcctgcatga agaaggccat cgccgcgatc
    14761 cacggcaaac ccgccatact gcgcttcttt ttatcgctca ttcaaagtcc tgttatcggg
    14821 ttatcactta tctggtgagc gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc
    14881 gctttggata atcgcatcga caatcgcctg gtgcagatcc agctttatca ctgtgtcgct
    14941 ggtaattgac gtgaagtaag tgtgataaac cgaatggaat agcgaggcga atgaggtcaa
    15001 aaacggattg gcgctcattt catagatatg ctcatgccag gccatatcga cttcgatcca
    15061 gcgttcacgg cgaaagttct cttttaatgc cgccatttcg gccattaacg tattgagatg
    15121 cgccttctgt tccgcggtgc caaccgttgc tgccagtagg caagcttgcg gctccagaca
    15181 gatacgcata accagaaagt gatcgatgac ctgatgaaag ttctcttctg tcatccacca
    15241 ggtaagcaat tcctgatcaa gaaaattcca gttcgattgt ggcatgactc gggtaccaat
    15301 tcgcggtcgc ggtaaaacca tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc
    15361 tgtacgactc actccaaatt gctcgcccag ctcaatctca ccaggcaaaa tggtgccggg
    15421 ttcatattca ccttttaaga tccgctgcgc cagcttttca gccagaacat acgaaaggtt
    15481 tttctgtgca gctaactgtt gtgcgcttaa aggcattact aatcttcctt tttcttttta
    15541 ttcctcctta gtatgccacc aggaagtgtg attacggttg caaaaacggc aaattgcttg
    15601 ttttatggca cattaacggg gcttttgctg aaaaaatgcg cggtcagaaa attattttaa
    15661 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    15721 ggcaaacacg ccgccgggtc agcgggattc tcctgagaat tccggcagag aaagcaaaaa
    15781 taaatgcttg actctgtagc gggagagcgt attatgcaca ccccgcgccg ctaagaaaaa
//
LOCUS       DACADA010000104        15785 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.106, whole genome shotgun sequence.
ACCESSION   DACADA010000104 DACADA010000000
VERSION     DACADA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.106"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..723)
                     /gene="phoU"
                     /locus_tag="ISB17_003239"
     CDS             complement(<1..723)
                     /gene="phoU"
                     /locus_tag="ISB17_003239"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP1981138.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(738..1511)
                     /gene="pstB"
                     /locus_tag="ISB17_003240"
     CDS             complement(738..1511)
                     /gene="pstB"
                     /locus_tag="ISB17_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAP1981139.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1602..2492)
                     /gene="pstA"
                     /locus_tag="ISB17_003241"
     CDS             complement(1602..2492)
                     /gene="pstA"
                     /locus_tag="ISB17_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP1981140.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2492..3451)
                     /gene="pstC"
                     /locus_tag="ISB17_003242"
     CDS             complement(2492..3451)
                     /gene="pstC"
                     /locus_tag="ISB17_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAP1981141.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3537..4577)
                     /gene="pstS"
                     /locus_tag="ISB17_003243"
     CDS             complement(3537..4577)
                     /gene="pstS"
                     /locus_tag="ISB17_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP1981142.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4891..6720)
                     /gene="glmS"
                     /locus_tag="ISB17_003244"
     CDS             complement(4891..6720)
                     /gene="glmS"
                     /locus_tag="ISB17_003244"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP1981143.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6882..8252)
                     /gene="glmU"
                     /locus_tag="ISB17_003245"
     CDS             complement(6882..8252)
                     /gene="glmU"
                     /locus_tag="ISB17_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP1981144.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8606..9025)
                     /gene="atpC"
                     /locus_tag="ISB17_003246"
     CDS             complement(8606..9025)
                     /gene="atpC"
                     /locus_tag="ISB17_003246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP1981145.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9046..10428)
                     /gene="atpD"
                     /locus_tag="ISB17_003247"
     CDS             complement(9046..10428)
                     /gene="atpD"
                     /locus_tag="ISB17_003247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP1981146.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10455..11318)
                     /gene="atpG"
                     /locus_tag="ISB17_003248"
     CDS             complement(10455..11318)
                     /gene="atpG"
                     /locus_tag="ISB17_003248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP1981147.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11369..12910)
                     /gene="atpA"
                     /locus_tag="ISB17_003249"
     CDS             complement(11369..12910)
                     /gene="atpA"
                     /locus_tag="ISB17_003249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP1981148.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12923..13456)
                     /gene="atpH"
                     /locus_tag="ISB17_003250"
     CDS             complement(12923..13456)
                     /gene="atpH"
                     /locus_tag="ISB17_003250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP1981149.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13471..13941)
                     /gene="atpF"
                     /locus_tag="ISB17_003251"
     CDS             complement(13471..13941)
                     /gene="atpF"
                     /locus_tag="ISB17_003251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP1981150.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14003..14242)
                     /gene="atpE"
                     /locus_tag="ISB17_003252"
     CDS             complement(14003..14242)
                     /gene="atpE"
                     /locus_tag="ISB17_003252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAP1981151.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14289..15104)
                     /gene="atpB"
                     /locus_tag="ISB17_003253"
     CDS             complement(14289..15104)
                     /gene="atpB"
                     /locus_tag="ISB17_003253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP1981152.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15113..15493)
                     /gene="atpI"
                     /locus_tag="ISB17_003254"
     CDS             complement(15113..15493)
                     /gene="atpI"
                     /locus_tag="ISB17_003254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAP1981153.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3783 a         4308 c         3951 g         3743 t
ORIGIN      
        1 tttatcgcta tctttttctg ccagcagttt atcgagctcg tcaccaccga cgtgacggaa
       61 atcctgcccc ttcacgtagt agaagataaa ctcacaaata ttctggcagc ggtcgccaat
      121 acgttcgata gaacgcgcac agaataacgc ggtaagtacg ctcggaatgg tacgcgaatc
      181 ttccatcatg taggtcatca gttgacgaac aataccttcg tactcctgat cgactttttt
      241 atcttcacga taaatacgta ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac
      301 gtcgtgcagc atctggatgg tatgacggcc cagcgactcc agacttacca gcaacggctg
      361 atgctgctgg gagaatttct ccagcgcagt acggcagatt ttatccgcca cgtcaccaat
      421 acgctccagc tcggcaatgg ttttactgat taccatcacc agacgcaggt cgctcgccgt
      481 cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt
      541 gacgttcttg tcgccttcga tgacgcgctt cgccagatcg ctgtcctggt tatgcatcgc
      601 ggtgattgca tcagaaagct gctgctccac catgccgccc atggtcatca cctgcgtgcg
      661 gatgctttcc agttcggcgt tgaactggcc ggaaatatgt ttattaagat tgagactgtc
      721 cataacgcac tcctgaatca accgtaacga ccggtgatgt agtcttctgt ttgttttttc
      781 gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa tcaattcgcc caggtacata
      841 aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg
      901 gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac gcccggtaga gatagggtcg
      961 agtgccgaac acggttcgtc gagcagcagc acttccgggc gaatggcgat accacgcgca
     1021 atgcacagac gctgttgctg accaccagag agagagtaac cgctctggtg caatttatct
     1081 ttggtttcgt tccacaatgc ggctttggtc aatgcccact gcacgcgctc gtccatgtcg
     1141 gcacgggaga gcttctcaaa cagacgcacg ccaaaggcga tgttgtcgta aatggacatc
     1201 gggaacggcg tcggtttctg gaacaccatg cccactttcg cacgcagcag ggcgatatcc
     1261 tgagagttgg tcaggatgtt gtcgccatcg agcagaattt caccttccgc acgctgctcc
     1321 gggtacagtt caaacatttt gttgaaggta cgcagcagcg ttgatttacc gcagccggac
     1381 ggcccgataa acgccgtaac ctggtttttg gcgatatcca ggttgatgtt tttcagggca
     1441 tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa ttttactcgg ggcagtttca
     1501 accatactca ttgcaatctt ttcctcatcg tgcctgatgc acttcgcgaa tcaggcttac
     1561 cgtaggccgg ataaggcgtt tacgccgcat ccagcaaagt gttaaccgtg tttattcttc
     1621 gcaaaaacaa cgcgcgccag aatgttcagc agcagtacgc acagggtaat gatcaacacc
     1681 ccggcccagg ccagttgctg ccattccgca aacgggctca tcgcaaactt aaagatcgtc
     1741 accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt ggagagcgca
     1801 gtaaacagca gcggtgcggt ttcaccagca atacgggcaa tcgccagcag gataccggtc
     1861 atgatcccgg acaccgacgc tttcagcgtg atcgaggcga tcatcttcca cttcggcgta
     1921 cccagcgcat aagccgcttc acgcaggctg tccggcacca gtttcagcat gttctcggtg
     1981 gtgcggataa caatcggcac ctgcaacagc gccagcgcaa tcacgcccgc ccagccggag
     2041 aagtgctcca tctgcgccac cacgatggtg taaacaaaaa gaccaaccac aatcgacggc
     2101 gcggagagca gaatgtcgtt aatgaagcga atcacttctg ccagccagga tttacgacca
     2161 tattcagcca ggtaaatccc cgccataatg cccagcggcg taccgaatac cgtagcccac
     2221 aaaattaaca ggccgctacc cgccagggcg ttcgccagac caccgccttc cgtattgggt
     2281 ggtggcgtca tttcggtgaa cagcgccagc gacataccgt cgataccgcg agtgatggtg
     2341 gacattaaaa tccagatcag ccagaacaga ccgaaggcca tcgtcgccat tgagagcgtc
     2401 agcgcaatac ggtttttgag gcggcgacgc gcctgcattt tgcggcggga ttcagccagt
     2461 gccgcagtgg tttgcatttc aaccatagcc attagcgtgc cccctcattc ttagccaggc
     2521 gcataatcat aaacttcgat gcggcgagga cgatgaaggt aatcacaaac aggatcaggc
     2581 ccagttccat cagtgcagca acgtgcagac ccgactctgc ttcggcaaat tcgttcgcca
     2641 gcgcagaggt gatactgttg cccggcatgt acagcgaggc gctgtcgagc tggtaggtgt
     2701 taccgatgat aaaggtcacc gccatggttt caccaagcgc gcggcccagc cccaacataa
     2761 tgccgccgat aacaccattt ttggtgaacg gaagaacgat acgccagata acttcccagg
     2821 tggtgcagcc aataccgtag gccgactctt tcatcatcac cggggtttgt tcgaacacat
     2881 cgcgcattac cgccgcaatg tacggaataa tcatgatggc gaggatcacg cctgccgcga
     2941 ggataccgat accaaaggcc gggccggaga acagcgcgcc gacaatcggg atattcgaca
     3001 tgatattgcc gaccggctcc tggaagtaaa cggcgaacag cggcgcaaag ataaacaggc
     3061 cccacatgcc gtaaacgata cttggaatgg ctgccagcag ctcaatggcg atacccagcg
     3121 ggcgcttcag ccagccaggc gcaagctcgg tcaggaacag ggcgataccg aaactcaccg
     3181 ggacggcgat cagcagcgcg ataaacgagg tcaccagcgt accgtagatc ggcaccagcg
     3241 ccccgtagat atcgttcggc gcatcccact ctttggtcca caggaaagcc agaccaaatt
     3301 tctgaatgct cggccaggag gagatgatca gagagacaat aatgccaccc aacatcaata
     3361 gcacaatcag cgccgccagt tttaccagca cgctgaaaat tatgtcgccc tttttacccg
     3421 gtgggttaaa agcaggcttg gttgcagcca taagttactc ttcagttaaa cgcgtttaca
     3481 aagtcatttt gtaatgccgg atgcggcgta aaacaccgta cccggcctga gttttattag
     3541 tacagcggct tgccgctact gtctttaata ttggtcttcc acgcagcgcg aacctgttcc
     3601 actacgctat ccggcaggct ggcgtaatcc aggtcgttcg cctgtttagc cccggttttg
     3661 tatgcccagt cgaagaactt cagcacttct gtgccttgtt ctggtttctt ctgatctttg
     3721 tggatcagaa tgaacgtggt agaggtaata ggccacgcat cttcgccttt ctggttggtc
     3781 agatcctgag cgaatgtttt gctccagtct gcgccttttg ctgcattagc gaagttttct
     3841 tcggtcggac taaccggttt gccatcagcg gagatcaatt tggtgtacgc caggttgttc
     3901 tgcttcgcgt aagcatattc aacgtaacca attgcacccg gcagacgctg aacgaacgcg
     3961 gcgataccgt cgttaccttt accgcccaga ccgatcggcc attttacggt agagccagta
     4021 ccaacgttgt ttttccactc ttcgttcact ttcgccaggt agctggtgaa gacgaaggaa
     4081 gtcccggagc catctgcgcg gcgtactaca gcaatgttct gtgaaggcag tttcagaccc
     4141 ggattcagtt tggcgatggc ttcatcatcc cacttcttga ttttgcccag gtagatgtcg
     4201 ccgagggttt taccatccag caccagttcg ccagacttca gacctggaat attaaccgcc
     4261 agcaccacgc cgccaatcac ggtcgggaac tggaacaggc cttcctgagc cagtttttca
     4321 tcagacagcg gcgcgtcaga ggcaccgaaa tcaacggtgt tagcgataat ctgttttacg
     4381 ccaccggaag aaccgatacc ctggtagtta actttattac cggtttcttt ctggtaagtg
     4441 tcagcccatt tggcatacac cggcgcaggg aaggttgcac ctgcacctgt caggcttgct
     4501 tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg cgacaacagt tgcgacggtg
     4561 gtacgcataa ctttcataat gtctcctggg aggattcata aagcattgtt tgttggctac
     4621 gagaagcaaa ataggacaaa caggtgacag ttatatgtaa ggtttatgac agttttatga
     4681 cagagagaga aagtctttag tctgatttaa ataagcgttg atattcagtt aattacaaat
     4741 attaataacg aagagatgac agaaaaattt tcattctgtg tcagagaaaa agtagccgaa
     4801 gatgacggtt tgtcacatgg agttggcagg atatttgatt aaaaacataa caggaagaaa
     4861 aatgccctgc ttacgcaggg catccattta ttactcaacc gtaaccgatt tcgccaggtt
     4921 acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg tggtaagcca gtagttgcag
     4981 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct caatgatgtg
     5041 catgttgtca ctgctcacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
     5101 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
     5161 aacgacgata accggcatat cggcatcaat cagcgccagc ggaccgtgtt tcagttcgcc
     5221 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg caccttccag
     5281 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
     5341 ttctgccagc gcttcaatgc gtttgtcctg agacagcatc tgctcaatac ggctcggcag
     5401 cgcctgcaga ccatgcacga tgtcatgttc aatggaggca tccagacctt tcaggcgaga
     5461 cagtttcgcc accagcatca acagcacagt taactgggtg gtgaatgctt tggtggaggc
     5521 cacgccaatt tccgtaccgg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
     5581 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgaaagacg
     5641 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
     5701 acgacgcacg gcagatttgc gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
     5761 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
     5821 ggcgaggatc tgaatatgct caaccttcga cagcagttca tcggcgttcg gtcccagctc
     5881 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttga tcgcgttcgg
     5941 ctgttcgtag atctctttct gcatgtagtg gcggtaaata cctttatcgc ccgcgtcata
     6001 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     6061 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa aacgacgagt
     6121 cactggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     6181 caccagcgga ctaccagaac gtgccgccag cagggtatcc ggatgacggg agtccatgat
     6241 cactgtaccg tacgcaccac gcagttgcgg gatagcacgc agaactgcct cacgcagagt
     6301 accgccttgt ttcagctccc agttcaccaa atgtgcaatc acttcggtgt cggtttcaga
     6361 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     6421 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     6481 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     6541 ttccgctgcc tgagccagca tctgtacttt accgaggcga cgcaggcggg tcatatgacc
     6601 ttcagcatcc accacggcca ggccggcaga gtcatatccg cgatattcca gacgacgtaa
     6661 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     6721 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     6781 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtgggc gggagattat
     6841 gttatctcct catcccatat gaccggatta cccgaccaga attacttttt ctttatcgga
     6901 cgacgccagc cttctttttg agtctgcggc acacggctga tagccagggc attttcgccg
     6961 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     7021 gccaccagtt gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     7081 ttcgcaccat cgtagttgca ggtaatggtt cccgcaccga tgttaacgtt atcgccaatt
     7141 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     7201 ttcatctcaa cgaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     7261 cgggcaaacg ggccaatagt acaggccgct gccagattcg catcttccac gacggtatat
     7321 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     7381 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     7441 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     7501 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag tcgggagagt
     7561 tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     7621 tcacgtcctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     7681 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     7741 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ttcaacaatg
     7801 ccggtaactt tgccgttttc acgggtgata cgtccataac cggtcggatc atccagtttt
     7861 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcagacgctg gagtgtttcg
     7921 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     7981 ggtgcggcct gctgcatcgc atgacccgta cccagctgtt ctgcctgaag cacccagttc
     8041 aggttgtcgt ctttcagcgc ctgcttaagc agatcgccgc cgtgaccata cacaaggtga
     8101 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     8161 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     8221 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     8281 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     8341 aaattgttca ataacggatt atccccgctg acgaaagcgc cattttcgac catcgtcacg
     8401 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     8461 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca tcaggatcta
     8521 tgtgaacgct tttcaggacg ggtcaatacg cgtaaaaaaa agccagcctg ttgccagact
     8581 ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca
     8641 gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag
     8701 agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat
     8761 cctggccgcg aattgcggtg tcggccagaa cggtcacgtt gccaggctgc acttcaagaa
     8821 tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac
     8881 caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc
     8941 tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga
     9001 cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc
     9061 tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc
     9121 gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt
     9181 acccggagaa ccggtgaata cctctgccac gaagaacggc tgggacagga agcgctggat
     9241 cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat
     9301 ggcgataatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt
     9361 gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg agtccagcgg
     9421 gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc
     9481 aaggtgcgca aaggtggttg ccggagacgg gtcagtcagg tcatccgcag gtacgtatac
     9541 cgcctgtacg gaggtgatag aaccggtttt ggtggaggtg atacgttcct gcagaacgcc
     9601 catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc
     9661 ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc
     9721 acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg
     9781 gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga taacgttgga
     9841 gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa
     9901 cacagagtaa ccggagtgct cgatcgcgat gttacgaata agctccatca tgtttacggt
     9961 tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat
    10021 caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta
    10081 ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc
    10141 gaccggttca cccagtacgt tcatgatacg acccagagtc gctttaccta ccgggacttc
    10201 gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat
    10261 tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc
    10321 attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc
    10381 gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct
    10441 acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta
    10501 atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca
    10561 ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca
    10621 accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc
    10681 ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat
    10741 gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg
    10801 ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt
    10861 accggaccga tcagttcgga caaggaaggg ttatccccca tgccggtgac ctgggcaaca
    10921 acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg
    10981 cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta
    11041 atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta
    11101 acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc aaggtgacca
    11161 atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta
    11221 cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg
    11281 cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc
    11341 ggcctgccca aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga
    11401 ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt
    11461 gcatcaacgg agcgtgatca cggtcgacgt aagccaacag agcggcttcg aagctgccga
    11521 ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact
    11581 gttgcgcaac agacatcggc gcatactgtt tctgcttcag cagttcggtc actttctgac
    11641 cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg
    11701 ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct
    11761 tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa
    11821 taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt
    11881 tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc agcgcagtca
    11941 gagaaccggt tttccctttc acttcacctt tggtgaaagc ttcaacgtat tcggcgttaa
    12001 cacgcgcagc acgctccagc agacgagagt ggaggtagaa tacgtcgccc gggaatgctt
    12061 cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag
    12121 acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcacccattg
    12181 cgcaaccagc gtacggtgcc aggtattgca gtgcagcgga ttcagacgcg gttgctacca
    12241 caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg
    12301 tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga
    12361 tgatggcatc gatagccagt gcggttttac cggtctgacg gtcaccgatg atcaattcac
    12421 gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc tgtaccggct
    12481 gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt
    12541 cgtgatccag cggaccttta ccatcgattg gtgcacccag agtgttaacc acacggccca
    12601 gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt
    12661 cggcaaggtc agcgtacggc cccataacaa ccgcacctac agagtcgcgc tcgaggttca
    12721 gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca
    12781 ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt
    12841 cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat
    12901 tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac
    12961 cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacacct gccattacag
    13021 acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag
    13081 aaattttcgc gagctgttgt tcactcaacg cggcagcgga aatgacgtct acctcagcgg
    13141 tagcctcact cacagcacgc aggtgaataa actgctccag aacatccggg agcgcgttaa
    13201 gacgaccatt ttcggccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac
    13261 cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct
    13321 ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt
    13381 ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg
    13441 taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat
    13501 gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc
    13561 caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc
    13621 cgcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcatc
    13681 cagaatctgc gagcggcgtt tgttagcctg ctcgatgatt acctgggctt ccgctttcgc
    13741 ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc
    13801 tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg
    13861 cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag
    13921 gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct
    13981 ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta
    14041 cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca
    14101 gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg aggatgccga
    14161 taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca
    14221 gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg
    14281 ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag
    14341 atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc
    14401 cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac
    14461 atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct taccccttca
    14521 aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc
    14581 gtgaagccgc cgatgccttt cattttgatg ctgtagaaca gaatcaggat aaatacgccc
    14641 agtgccatag acagcgttac gttcacgtca gcagacggaa ccacacgcag tgcaggcaga
    14701 cccagtacat gttcagcaat gtacggcagc aggtcgatag gcagtaaatc catcaggttc
    14761 atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca
    14821 tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga
    14881 aacttacctg gcacgccgct ggttgccttt ttggctacac tacggaataa aaccaggaac
    14941 aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct
    15001 ggggggttat gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg
    15061 tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt
    15121 tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga
    15181 tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg
    15241 ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc cacccggcct ttcgctggtg
    15301 tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca
    15361 ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac
    15421 ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga
    15481 gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata
    15541 aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata cggtgcgccc
    15601 ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg
    15661 catacaattg cgacttttct gcaaaccctg ttcgatcatg aaaaactgta aataacgcgt
    15721 aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat
    15781 ttatt
//
LOCUS       DACADA010000105        15677 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.107, whole genome shotgun sequence.
ACCESSION   DACADA010000105 DACADA010000000
VERSION     DACADA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.107"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            491..2419
                     /gene="thrS"
                     /locus_tag="ISB17_003255"
     CDS             491..2419
                     /gene="thrS"
                     /locus_tag="ISB17_003255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP1981154.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2423..2965
                     /gene="infC"
                     /locus_tag="ISB17_003256"
     CDS             2423..2965
                     /gene="infC"
                     /locus_tag="ISB17_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP1981155.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3062..3259
                     /gene="rpmI"
                     /locus_tag="ISB17_003257"
     CDS             3062..3259
                     /gene="rpmI"
                     /locus_tag="ISB17_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP1981156.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3312..3668
                     /gene="rplT"
                     /locus_tag="ISB17_003258"
     CDS             3312..3668
                     /gene="rplT"
                     /locus_tag="ISB17_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP1981157.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3791..3835
                     /locus_tag="ISB17_003259"
     CDS             3791..3835
                     /locus_tag="ISB17_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAP1981158.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4119..5102
                     /gene="pheS"
                     /locus_tag="ISB17_003260"
     CDS             4119..5102
                     /gene="pheS"
                     /locus_tag="ISB17_003260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP1981159.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5117..7504
                     /gene="pheT"
                     /locus_tag="ISB17_003261"
     CDS             5117..7504
                     /gene="pheT"
                     /locus_tag="ISB17_003261"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP1981160.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7509..7808
                     /gene="ihfA"
                     /locus_tag="ISB17_003262"
     CDS             7509..7808
                     /gene="ihfA"
                     /locus_tag="ISB17_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAP1981161.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7909..8889
                     /gene="btuC"
                     /locus_tag="ISB17_003263"
     CDS             7909..8889
                     /gene="btuC"
                     /locus_tag="ISB17_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAP1981162.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8952..9503
                     /gene="btuE"
                     /locus_tag="ISB17_003264"
     CDS             8952..9503
                     /gene="btuE"
                     /locus_tag="ISB17_003264"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAP1981163.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9503..10252
                     /gene="btuD"
                     /locus_tag="ISB17_003265"
     CDS             9503..10252
                     /gene="btuD"
                     /locus_tag="ISB17_003265"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAP1981164.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10330..10794
                     /locus_tag="ISB17_003266"
     CDS             10330..10794
                     /locus_tag="ISB17_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP1981165.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11042..11755
                     /gene="ydiV"
                     /locus_tag="ISB17_003267"
     CDS             11042..11755
                     /gene="ydiV"
                     /locus_tag="ISB17_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAP1981166.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11818..13254
                     /locus_tag="ISB17_003268"
     CDS             11818..13254
                     /locus_tag="ISB17_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAP1981167.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13258..13449)
                     /gene="hemP"
                     /locus_tag="ISB17_003269"
     CDS             complement(13258..13449)
                     /gene="hemP"
                     /locus_tag="ISB17_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAP1981168.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13581..14627)
                     /gene="aroH"
                     /locus_tag="ISB17_003270"
     CDS             complement(13581..14627)
                     /gene="aroH"
                     /locus_tag="ISB17_003270"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAP1981169.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(14784..15617)
                     /gene="ppsR"
                     /locus_tag="ISB17_003271"
     CDS             complement(14784..15617)
                     /gene="ppsR"
                     /locus_tag="ISB17_003271"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAP1981170.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         3913 a         3704 c         4177 g         3883 t
ORIGIN      
        1 gaggatgatg attaattatt atcgccagaa cgaaatgcgc acgaaaaatt gctataagaa
       61 ttgtgtcact tttatcattg cgatatgacc acttcgtcac tgaatctcat gatcgtttaa
      121 ataccaccgc caaataacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc
      181 gctcgtagta aacctttcag acactctgtg atgatatcag tggttcttta agcgttttgc
      241 tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc
      301 tccctttttt tagtgttaac atcgttcaac cgggtggaag gtgagaggtt aatgtctttc
      361 atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa
      421 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata
      481 aggatatata atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc
      541 tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc
      601 agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact
      661 gtcgatcatt accgccaaag acgaagaagg tctggagatc attcgtcact cctgtgcgca
      721 cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt
      781 tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt
      841 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa
      901 gaaagtcagc tggcatgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt
      961 ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga
     1021 atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa
     1081 actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg
     1141 tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc aacgcctgga
     1201 agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca
     1261 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact
     1321 ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt
     1381 catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat
     1441 gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccggggca
     1501 cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga
     1561 gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga tgcgcgtgcg
     1621 tggttttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt
     1681 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt
     1741 cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc
     1801 tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg
     1861 tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc
     1921 atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga gcgcctccta
     1981 tgtgggcgaa gacaacgagc gtaaggttcc ggtaatgatt caccgcgcaa ttctggggtc
     2041 gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct
     2101 tgcgccggtt caggttgtta tcatgaatat taccgattca cagtctgatt acgttaacga
     2161 attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa
     2221 gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg
     2281 tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg
     2341 aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct
     2401 taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa
     2461 ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca
     2521 gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt
     2581 agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt
     2641 cctctatgaa aagagtaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt
     2701 taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa aactccgcag
     2761 cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg
     2821 tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca
     2881 agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt
     2941 gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agcctttggg
     3001 cttcacaggc attgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa
     3061 aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg
     3121 tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg
     3181 taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc
     3241 gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca
     3301 ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa
     3361 ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc
     3421 aggctgttat caaagctggt cagtatgctt accgcgaccg tcgtcaacgt aagcgtcagt
     3481 tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaatggt atttcttaca
     3541 gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg
     3601 atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc
     3661 tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa cgccatcaaa
     3721 acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt
     3781 aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca cctgactcca
     3841 ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc
     3901 tttttttgta tgcgcgtttt gaaaaataaa aagcctgtct catcaggcgt aattgtcgca
     3961 gccagtctgg acacggccag cgcggagaaa ccggaccgta cttaagtacg tgaggatttc
     4021 gagcactgcc cgggaccaaa atggcaagta aaatagcctg atgggatggg ctctaagtcc
     4081 aacgaaccag tgtcaccact gacacaatga ggaaaaacat gtcacatctc gcagaactgg
     4141 ttgccagtgc gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc
     4201 gcgtcgaata tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc
     4261 tgccgccaga agagcgtccg gcagctggtg cggttatcaa tgaagcgaaa gagcaggttc
     4321 agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc agcactgaat gcgcgtctgg
     4381 cggcggaaac gattgatgtc tccctgcctg gtcgtcgcat tgaaaacggc gggctgcatc
     4441 cggttacccg taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg
     4501 caaccgggcc ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc
     4561 accaccctgc gcgcgctgac cacgacactt tctggtttga cgctacccgc ctgctgcgta
     4621 cccagacctc tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca
     4681 tcgcgcctgg ccgcgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc
     4741 agatggaagg tctgattgtt gataccaaca tcagctttac taacctgaaa ggcacgctgc
     4801 acgacttcct gcgtaacttc tttgaggaag atttgcagat ccgcttccgt ccttcctact
     4861 tcccgtttac cgaaccttct gcagaagtgg acgtcatggg taaaaacgga aaatggctgg
     4921 aagtactggg ctgcgggatg gtgcatccga acgtgctgcg taacgttggc atcgacccgg
     4981 aagtttactc tggtttcgcc ttcgggatgg ggatggagcg tctgactatg ttgcgctacg
     5041 gcgtcaccga cctgcgttca ttctttgaaa acgatctgcg tttcctcaaa cagtttaaat
     5101 aaggcaggaa tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc
     5161 gattgatagc gacgcgctgg caaatcaaat cacgatggcg ggcctggaag ttgatggtgt
     5221 agaaccggtt gccggtagct tccacggcgt ggttgtgggt gaagtggttg agtgtgcgca
     5281 gcatccgaac gctgacaaac tgcgtgtgac aaaagtgaat gtcggcggcg atcgcctgct
     5341 ggacatcgtc tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagcgaccat
     5401 tggtgctgtt ctgccgggtg atttcaaaat taaagccgcg aaactgcgtg gcgaaccgtc
     5461 tgaagggatg ctgtgctcct tctctgagct gggaatttct gacgatcata acggcattat
     5521 cgagctgcct gcggatgcgc cgattggcac cgacatccgc gaatacctga aactcgatga
     5581 caacaccatc gaaatcagcg tgacaccaaa ccgtgccgac tgcttaggta tcattggtgt
     5641 tgcgcgcgac gttgccgtgt tgaaccagct gccgctggtt gaaccggaaa tcgttccggt
     5701 tggtgcgacc atcgacgaca cgctgccgat tgcagtagaa gcgccggatg cctgcccgcg
     5761 ctatcttggc cgtgttgtaa aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa
     5821 agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg tcaccaacta
     5881 tgtgctgctc gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg
     5941 cattgtggtg cggatggcaa aagagggtga aacgctggta ttgctcgacg gtactgaagc
     6001 gaagctgaat gctgacaccc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg
     6061 catatttggt ggcgaacact ctggcgtgaa tgacgaaacg caaaacgttc tgctggaatg
     6121 cgctttcttc agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc tgcatactga
     6181 tgcgtctcac cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg
     6241 tgcgacccgt ctgctgatcg acatctgcgg tggtgaagcg ggtccggtaa ttgatatcac
     6301 taacgaagca acgctgccga agcgtgcaac catcacttta cgtcgtagca aactggatcg
     6361 cctgatcggc catcatattg ctgatgagca ggtaacagac attctgcgtc gtctcggctg
     6421 cgaagtgacc gaaggcaaag acgagtggca ggcagttgcg ccgagctggc gtttcgacat
     6481 ggagattgaa gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc
     6541 ggatgagccg gtacaggcaa gcctgattat ggggattcac cgtgaagctg acctgtcgct
     6601 caagcgcgta aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt
     6661 cgttgatccg aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctgccaag
     6721 cccgatctct gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac
     6781 cgtggtgtac aaccagaacc gtcagcagaa tcgtgtacgc attttcgaaa gcggtctgcg
     6841 tttcgtgcca gatactcagg caccgttggg tattcgtcag gatctgatgt tagccggtgt
     6901 gatttgcggt aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta
     6961 tgatttgaaa ggcgatcttg aatccgttct cgacctgacc ggtaaactga atgaggttga
     7021 gttccgtgca gaagcgaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa
     7081 aggtgaacgt attggttttg ttggggttgt tcatcctgaa ctggaacgta aactggatct
     7141 taacggtcgc actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc
     7201 tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc
     7261 agaaaacgtt cccgcagcgg atattttatc cgaatgtaag aaagttggcg taaatcaggt
     7321 agttggcgta aacttatttg acgtgtaccg cggtaagggt gttgcggagg ggtataagag
     7381 cctcgccata agcctgatcc tgcaagatac cagccgtaca ctcgaagaag aggagattgc
     7441 cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga ttccaggcat cattgaggga
     7501 ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat aagcttgggc
     7561 ttagcaagcg ggatgccaaa gaactggttg aactgttttt cgaagagatc cgtcgcgctc
     7621 tggaaaacgg cgaacaggtg aaactctctg gttttggtaa cttcgatctg cgtgataaga
     7681 atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg
     7741 tggtgacctt cagacccggg cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag
     7801 acgagtaatc tgatctaact aaaaaggccg ctctgcggcc ttttttcttt tcactgtcga
     7861 agagtcaccg taaaatcaac gccatgacac ttcagcagaa cggataccat gctgacactt
     7921 gcccgccaac aacagcgaca aaatattcgc tggttattat gcctgtcagt tttgatgctg
     7981 ctggcgcttc tcttaagcct ttgcgccggt gaacaatgga tctcgccagg tgactggttt
     8041 actcctcgtg gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct ggctgtattg
     8101 ctggttggtg ctgcgttggc tatatccggc gctgtaatgc aggcgttgtt tgaaaatcct
     8161 ctggcagaac ctggactact tggcgtctct aacggcgcag gcgtggggct tatcgccgcg
     8221 gtattgcttg ggcaagggca actccccaac tgggcgctgg ggctgtgtgc gattgctggc
     8281 gcgcttatca tcactttaat actcttacgt ttcgcccgtc gtcatctttc gaccagtcgg
     8341 ttattgctgg ctggcgttgc attagggatt atctgtagtg cactaatgac gtgggctatc
     8401 tacttttcca cctctgttga tttacgtcag ctgatgtact ggatgatggg cggttttggc
     8461 ggcgtagact ggcggcaaag ctggctgatg ctggcattga tccccgtgtt gttgtggatc
     8521 tgttgtcagt ccaggccggt gaatatgtta gcacttggcg agatctcggc gcggcaactg
     8581 ggtttacccc tgtggttctg gcgcaatgtg ttggtcgcag cgaccggctg gatggttggc
     8641 gtcagtgtgg cgctggcggg tgctatcggc tttattggtc tggtgatccc acatattctc
     8701 cggctgtgtg gtttaaccga tcatcgcgta ttacttcccg gctgcgcgct ggcaggggcg
     8761 agcgcattgc tgctggccga tattgtagcg cgcctggcat tagctgccgc agagctgcct
     8821 attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta tctggttatt gttaaaagca
     8881 ggacgttagc cgcaaaaaga cggtctatga ttaaaagcta agatttttac tgaccacacc
     8941 caggagaaac gatgcaagat tccattctga cgaccgtagt gaaagatatc gacggtgaag
     9001 tgaccacgct ggagaagtac gccggtaatg tgctgttgat tgtcaatgtc gcctcaaagt
     9061 gtggcttaac gccgcaatat gagcagttgg agaatattca gaaagcctgg gccgatcgtg
     9121 gttttgtggt gctgggattc ccgtgcaacc agtttctgga acaagaaccg ggcagcgatg
     9181 aagagattaa aacttactgt accaccacat ggggggtgac gttcccgatg ttcagtaaga
     9241 ttgaagttaa tggcgaagga cgccatccgc tgtatcaaaa attgattgcc gcagcgccga
     9301 ccgcagtcgc gccggaagag agcggattct atgcccgtat ggtcagcaaa ggtcgtgcac
     9361 cgctgtaccc tgatgatatt ttatggaatt ttgagaaatt tttagttggc agggacggat
     9421 tggtcatcca gcgtttttcc ccggatatga cgccggaaga tcccattgtg atggaaagca
     9481 ttaaactggc gttggcaaaa taatgtctat tgtgatgcag ttacaggatg ttgcggaatc
     9541 tacccgcctg gggccgcttt ctggcgaggt tcgggctggg gagatcctgc acctggtggg
     9601 gccgaacggc gcgggtaaga gtaccttgct tgcacgaatg gccggaatga ccagcggtaa
     9661 gggaagcatt cagttcgcgg ggcaaccact ggaagcatgg tctgcaacaa aactcgcgct
     9721 gcatcgcgcc tatctttcac aacagcagac gccgccgttt gcaatgccgg tctggcacta
     9781 cctgacactg catcagcacg ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc
     9841 gctggctctt gatgacaaac tcggacgtag caccaatcaa ctttccggcg gtgaatggca
     9901 acgcgtacgt cttgctgcgg tggtgcttca aatcacacca caagctaatc ccgcaggcca
     9961 attgctgctt cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa atgcgttaga
    10021 caaaattctg agcgcgctgt gtcagcaagg actggcgatt gtgatgagca gtcacgatct
    10081 caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc
    10141 cagtggacgc agagaagagg tgctcacgcc ggcaaatctg gcacaggcct atgggatgaa
    10201 ttttcgccgt ctggatatcg aaggtcacag aatgctgatt tcgaccatct gatttcatta
    10261 gtatcttgaa aatcgggtaa ttacgacgct aaattaatgc aagaataaaa aacagaggaa
    10321 ccgtacggaa tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc
    10381 caccataaag caccgccgcc aaatgccagg ctttctgatt cgatcaccgt tattgccggt
    10441 ttgaacgacc agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg
    10501 cgcggtgtgg actgttcggg atttgtggtt gtgacgatgc gcgatcgttt cgatttgcag
    10561 ctgccccgtg aaaccaaaca acaagcctct attggcacgc aaattgataa agacgagttg
    10621 ctgccaggtg acctggtctt tttcaaaacg ggttccggac aaaatggttt gcatgtaggt
    10681 atttacgata ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc
    10741 tcacttgata atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc
    10801 gaaaaatgga cggcgatgat acatcgccgt ccatttactc ctgaatacag tagtattttg
    10861 cagacagaaa ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaagga
    10921 cccctgaaaa agtggttaac atggaacaaa tgaaagttac tccttctgta attgttatgt
    10981 cttatggcct catccctttt agccggatac tgaaaaacat ccttcgagag ggacggttac
    11041 catgaagatt tttttggaga atctttatca ttctgattgc tatttcctgc cgataagaga
    11101 taaccagcaa gaccttgtcg gcgttgagtt aattacccat ttctccagtg aagatggtac
    11161 ggttcgtatc ccgacgagtc gggttatagc gcaacttacc gaggagcaac attggcaatt
    11221 attttctgaa cagttggaat tactaaaatc atgccaacac ttttttattc aacataaact
    11281 ctttgcctgg cttaatttaa cgccacaagt ggctacatta ttattagaca gggataattt
    11341 cgcaggcgaa ctattaaaat atccgtttat tgaactgttg attaatgaga attacccgca
    11401 tttcaatgaa ggaaaagata atcgcgatct tttatcctta tcgcaaatgt atccccttgt
    11461 gttaggtaat ttgggagcag gtaatagcac aatgaaagcg gtgtttgatg gtcttttcac
    11521 ccgcgtcatg ctggataaaa gttttattca gcaacaaatc acgcatcgct ccttcgaacc
    11581 gtttattcgt gcgatccagg cgcaaatttc cccttgttgc aattgcatca tcgcgggggg
    11641 cattgatacg ccagaaatat tggcacagat tatacccttt gatttccatg ctctccaggg
    11701 atgcctgtgg cctgcggttc ctataaatca gataacgacg ttggttcagc gataaccctc
    11761 ctgtttgctg gtgtttagga cgagagtaac cgtctacact atcaaacagg aggatctatg
    11821 accctgtctt ttattacccg ctggcgcgat gaattgccag aaacctatac agcactttcc
    11881 cctacgcctt taaataatgc ccggctgatt tggcataata ccgaactggc taacacgctg
    11941 agtattccat cgtcgctgtt taaaaatggc gcaggtgtct ggggcggcga aaacctactg
    12001 cctggtatgt caccactggc gcaggtttac agtggtcatc agtttggcgt ctgggcgggc
    12061 caactgggtg atgggcgcgg cattttactc ggcgaacaac tgcttgctga tggcactaca
    12121 atggactggc atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg
    12181 gcggttttac gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc
    12241 attccgacga cccgcgcttt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg
    12301 gtggagccag gcgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc
    12361 gaacattttt actatcgccg cgagccggaa aaggttcgtc agttagctga ctttgccatt
    12421 cgtcattact ggccacatct tgcagatgat gaggacaaat accgtctctg gtttaccgat
    12481 gttgtcgcac gtaccgcctc gttaattgcc caatggcaga ctgtcggctt tgctcatggg
    12541 gtgatgaata ccgacaacat gtcgctgctg gggctgacgc ttgattacgg gccgtttggt
    12601 tttcttgatg attacgaacc cggttttatt tgtaatcact cggatcatca agggcgttac
    12661 agctttgata atcaacctgc cgtcgcgttg tggaatttac agcgtctggc gcagacattg
    12721 tcaccatttg ttgccgtaga tggcctgaat gaggccctgg acagctatca gcaggttttg
    12781 ttgacgcatt atggacaacg gatgcggcag aaactgggct tcatgacgga gctaaaagag
    12841 gataacgcgc tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc
    12901 cgcacattcc gcatgctgag tctgaccgag cagcacagcg cggcgtcacc gctacgtgat
    12961 gagtttattg atcgtgcagc atttgatgac tggtttgccc gttatcgggg gcgtttgcaa
    13021 caagacgaga ttaccgataa tgagcgtcag caactgatgc aaagcgttaa cccggctctg
    13081 gtgttgcgca actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg
    13141 gaattgcacc gcctgcatga ggcgttgcga aatcctttca gcgacagaga tgatgactat
    13201 gtcagtcgtc cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta
    13261 cttggtcaac agcagcttgc cagcctgagt tttacggagc agatattctt gtccgtcatg
    13321 atcgataatc agtttgccat ccggacctaa cagtgtctgg ctggtaattc gccggatggg
    13381 ctgcggggaa gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt
    13441 ataacgcata aaaattaata ataatttata taacaatgat tcttattatc aatattatgg
    13501 agggccagag caagcagtaa tttgttgaga agacaacaag ttgcgcagaa atgcccatcc
    13561 tgtcaggaat gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc
    13621 aggcgttcgg tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc
    13681 ggctgaccgc cgacgatttt ttgcgttcct tcgcgcagga aactttccgc cataattccg
    13741 gcaatcgccg tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg
    13801 cgacggtgct gcttctggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggcaaa
    13861 tcaaattcgt gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt
    13921 ttgccgccac gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc
    13981 tgaccgtttt tgtctggcga gaggaacata tggctagcgc gggctgcgcg gatagcatcc
    14041 acggcaatcc gcgtgttgcc atcggtacca tttttaaaac ccaccggaca ggagagtgcc
    14101 gaagccattt cgcggtggat ctgactttcg gtagtacgtg cgccaatcgc gccccaactg
    14161 attaaatcag caataaactg accggtcacc atatcgagga actcagtcgc ggtagggacg
    14221 cccagctcat ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg atttacccga
    14281 tagctaccgt ttaaatctgg atcggagatg agtcctttcc agccgacaac cgttcgtggt
    14341 ttttcaaaat aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac
    14401 tgcagacggg tggcgtactc catcgcagcg gtgagatcgt ggatcgagca ggggccaata
    14461 atgaccaaca gtcgcttatc ttcaccattc agtatttttt caattctacg gcgggagtcg
    14521 gtgacatggg tggcgacgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt
    14581 accaggctct caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga
    14641 aatttgttgc ttctacggca ggactaccgg gaagtgatgc gcatcacgat aaaccaatcg
    14701 cagctgattt caagtgcgag gtgtgtcaca cctcgccggg tggttagcat gataacaaaa
    14761 aaacaagcta atgcactagt tctctagtac attcggcggc taaggcccat gatatcgagg
    14821 atcttggtgg caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt
    14881 ttacggtaca acgcttccac ttccgcgact tccatcctgc actgacgaag cgaggcatag
    14941 cgactgttct cccgacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg
    15001 ccgaacaatt tatgctgaag tggtttgagc gacgcgggta gcaccagatt atccatatca
    15061 tcggcaataa aggggtagtt tgccgcgcgg ataccaaatt gcattgccag atacagactg
    15121 gtgggggttt taccacagcg agaaacaccg agcaggatca cctgagcctg gtccagattg
    15181 cgcaacgaaa taccgtcatc gtgggcaagg gtgtaatcaa tcgccgcaat gcgcgcatca
    15241 tatttattga gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc
    15301 agtttcatct cttgttgtag tggggcaacc agcgcctgaa cgatatcctg gcaaaagcct
    15361 tcactttgca agatgatagc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg
    15421 cgcacacctg tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc
    15481 tcattttcga caaacggcag cgtaatgctg ctgatagtga cgggaaattg tgacattact
    15541 gcgtgtccta atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga
    15601 tcaacagcat tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa
    15661 attaaaaacg cacagaa
//
LOCUS       DACADA010000106        15572 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.108, whole genome shotgun sequence.
ACCESSION   DACADA010000106 DACADA010000000
VERSION     DACADA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.108"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            240..449
                     /gene="mokC"
                     /locus_tag="ISB17_003272"
     CDS             240..449
                     /gene="mokC"
                     /locus_tag="ISB17_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAP1981171.1"
                     /translation="MQNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(553..1683)
                     /gene="dnaJ"
                     /locus_tag="ISB17_003273"
     CDS             complement(553..1683)
                     /gene="dnaJ"
                     /locus_tag="ISB17_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP1981172.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1772..3688)
                     /gene="dnaK"
                     /locus_tag="ISB17_003274"
     CDS             complement(1772..3688)
                     /gene="dnaK"
                     /locus_tag="ISB17_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP1981173.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4059..4463
                     /locus_tag="ISB17_003275"
     CDS             4059..4463
                     /locus_tag="ISB17_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAP1981174.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4489..5202
                     /gene="msyB"
                     /locus_tag="ISB17_003276"
     CDS             4489..5202
                     /gene="msyB"
                     /locus_tag="ISB17_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAP1981175.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5351..5917
                     /gene="satP"
                     /locus_tag="ISB17_003277"
     CDS             5351..5917
                     /gene="satP"
                     /locus_tag="ISB17_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAP1981176.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5952..6539)
                     /gene="mog"
                     /locus_tag="ISB17_003278"
     CDS             complement(5952..6539)
                     /gene="mog"
                     /locus_tag="ISB17_003278"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAP1981177.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6654..7607)
                     /gene="tal"
                     /locus_tag="ISB17_003279"
     CDS             complement(6654..7607)
                     /gene="tal"
                     /locus_tag="ISB17_003279"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP1981178.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7886..9316
                     /locus_tag="ISB17_003280"
     CDS             7886..9316
                     /locus_tag="ISB17_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAP1981179.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9386..10162
                     /gene="yaaA"
                     /locus_tag="ISB17_003281"
     CDS             9386..10162
                     /gene="yaaA"
                     /locus_tag="ISB17_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAP1981180.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10202..10498)
                     /locus_tag="ISB17_003282"
     CDS             complement(10202..10498)
                     /locus_tag="ISB17_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP1981181.1"
                     /translation="MKKMQSIVLALSLVLFAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10712..11998)
                     /gene="thrC"
                     /locus_tag="ISB17_003283"
     CDS             complement(10712..11998)
                     /gene="thrC"
                     /locus_tag="ISB17_003283"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAP1981182.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHGGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11999..12931)
                     /gene="thrB"
                     /locus_tag="ISB17_003284"
     CDS             complement(11999..12931)
                     /gene="thrB"
                     /locus_tag="ISB17_003284"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAP1981183.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(12933..15395)
                     /gene="thrA"
                     /locus_tag="ISB17_003285"
     CDS             complement(12933..15395)
                     /gene="thrA"
                     /locus_tag="ISB17_003285"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAP1981184.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15477..15542)
                     /gene="thrL"
                     /locus_tag="ISB17_003286"
     CDS             complement(15477..15542)
                     /gene="thrL"
                     /locus_tag="ISB17_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAP1981185.1"
                     /translation="MKRISTTITTTITITTGNGAG"
BASE COUNT         3624 a         4151 c         3868 g         3929 t
ORIGIN      
        1 aggaaaacac cttttttgtg aatccctgga tatatttaag ttaaataaat tgatgtattg
       61 aaatattatt ccgcaaaaat gcgaggcgtc ttcagaaaaa gaattgataa aacagaagtg
      121 aattaaaagc gcagtataat agcggcgggt gcttgaggct gtctgtctca ggcattggct
      181 gaacggcaga tagagaaaag ccccgagtga tattttacca tcaacccgag gccccctata
      241 tgcagaacac atgtagagtg cctcttactg accgaaaggt caaggagaag agagcaatga
      301 agcagcataa ggcgatgatt gtcgccctga tcgtcatctg tatcaccgcc gtagtggcgg
      361 cgctggtaac gagaaaagac ctctgtgagg ttcacatccg aactggccag acggaggttg
      421 ctgttttcac ggcttacgaa tccgagtaag agcaacggcg gggagtgatc cccgccactt
      481 tacaggtgct cgcatatctt caacgcaccc ttttttttac ctggcctgcc cacgggcagg
      541 cttttgggga ggttagcgag tcaggtcgtc gaaaaacttc ttcacaccat caaagaagct
      601 ctttgagcgc gggctgttgt gctcgccggt tgggccacca aagctttctt gcagctcttg
      661 cagcagctgt ttctgcttct cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag
      721 caaatcaccc tgtgcgccgc cgcggacaga cttgacgcct ttaccacgca tacggaacag
      781 cttaccggtc tgggtttcgc caggaacttt cagtttaacg cgaccatcaa gggtcggcac
      841 ttcgatttcg ccgcccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt
      901 gttgccttca cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acaggtcgcc
      961 tgccggtgcg ccgtgttcgc ccgcttcacc ttcgcccgca agacggatgc ggtctccagt
     1021 gtccacccct gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc
     1081 atgacatttg ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt
     1141 ctgctgcacg gcaaagaaac cctggcgcat ctgcacctgg ccagaaccat gacaggtcgg
     1201 acaggtctgc ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc
     1261 cagagtcgga atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc
     1321 catgttatag cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa
     1381 aatatcgccg aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccgcc
     1441 gccgcccatg ccgccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt
     1501 ttgcgagtcg gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc
     1561 tttgtcaccc tggttacggt ccgggtggta tttcatggcc aggcgtttgt aggccttttt
     1621 gatttcacgc tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc
     1681 catctttttt gatttgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc
     1741 gtgtcagtat aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt
     1801 cagcgtcgac aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct
     1861 gctgctgggc atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt
     1921 cctgcatttt cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag
     1981 tcagcgcaga ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa
     2041 cctgcttacg ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt
     2101 caaacttacg gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat
     2161 cttcgttcag accagaagaa gccttgatag tgatcttctg ctctttaccg ctgtttttgt
     2221 ctttcgcgga aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg
     2281 gcatgccgcg cggtgccggg ttgataccat ccaggttgaa ctgacccaga gatttgttat
     2341 cagccgcacg tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt
     2401 cagcggtaga gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg
     2461 tggtcatcac accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca
     2521 gtacgtcttt tacgtcacca gtcagaacac caccctgaac agcagcaccg atggctacag
     2581 cttcgtccgg gttaacatct ttacgcggct ctttaccaaa gaattcagca actttcttct
     2641 gaaccattgg catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg
     2701 acaggccagc gtcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt
     2761 caaccaggct ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg
     2821 cgtctgcagt gatgtacggc aggttaacgt cggtctgctg agcggaagac agttcgattt
     2881 tcgctttttc tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa
     2941 tgccctgatc tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt
     3001 caccacccag gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa
     3061 cttcgtcgat ttcgataatg gaaatatcga aagtaccacc acccaggtca taaaccgcga
     3121 tagtacggtt gccagtacct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt
     3181 tgatgatacg ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct
     3241 gagcatcgtt aaagtatgcc ggtacggtaa taacagcttc agttaccggt tcacccaggt
     3301 aatcttcagc ggttttcttc atttttttca gcacttcagc agagatctgc ggcggtgcca
     3361 ttttctggcc tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg
     3421 gcatgatgga aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt
     3481 taatcgcaaa cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa
     3541 ccagagtttc accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg
     3601 cgttctccag tacacgagga gtggtgccat ccataatcgc tacacaagag ttggtagtac
     3661 ccaggtcgat accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat
     3721 gtggttgtga gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt
     3781 ttgcagactc actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg
     3841 ggagatgcaa aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac
     3901 gtgaaactga cagcaatttt attcatataa agaatgaatc gcagcaaggc attcatcaaa
     3961 aaattgtaat aaaaagaaaa tattacgtgc ctgaaacttc tctttatcag cggtaaacta
     4021 gtttcatccc cttacccctc cccaccaaga gaatccttat gaaatccgtt attacgattt
     4081 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaaa
     4141 tcctcggcgt cattgcaatg ccgcgaaacg aaaccaacga tctggcgctg aaacttcctg
     4201 tttgtcgcat tgttaaacgc atacaactct cagccgacca tggcgatttg cagttaagcg
     4261 gtgcatcgat ttatttcaaa gccacccgaa gcgccagtca gaccctgaat attccttcag
     4321 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaacaaac
     4381 gctgcgtctc aaaaatcacc ttttccggtc atacagtgaa ctcatcggat atggccacgc
     4441 tgaaaattat cggcgacgac taacgccaga atatagaaac cacaaaaaat gaatgttaat
     4501 tacctgaatg attcagatct ggattttctc cagcattgta gtgaggaaca gttggcaaat
     4561 ttcgcccggc tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg
     4621 cgcaacgaac tgtttaaatc gatggaagga catcccgagc gacaccgccg caactggcag
     4681 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     4741 cacggtaaat tgtatcgcgc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     4801 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     4861 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     4921 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     4981 gtgtcgcatt tgctttccag ccaattgacc cgcattttac gcacccacgc agcaatgagc
     5041 gtgcttgggc atggtttgct gcgcggcgcg ggtctgggag gccctgtggg cgcagcatta
     5101 aatggggtta aagcggtcag cggcagtgcc tatcgcgtga caattccagc cgtactgcaa
     5161 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     5221 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     5281 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     5341 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     5401 cggcatgacc accattctgc ttaacctgca caacgtgggt tattttgctc tggacggtat
     5461 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgccg gtctgctgga
     5521 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     5581 gacgctggtt gcaattctgc tgatgccgaa actggggctg accgatgcgc caaatgcaca
     5641 gttccttggt gtctacctgg gtctgtgggg catatttacg ctgtttatgt tcttcggcac
     5701 gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     5761 gctggcgatc ggtaacattg ccggtaacgc cgcgatcatc cactttgccg gctggattgg
     5821 tcttatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     5881 tggtcgcacc gttctgccga ttggtgaatc ccactaattg ttttgagatc cccccgctcg
     5941 gggggatttt tttattcgct aacttcgcgt cttgcactct tcggtctgaa tgctgcaacc
     6001 acttccggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     6061 gcaaaaatac cgtgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     6121 ttaatagact tcggttgacc gggtaagtta aggatcagcg cctgtttgcg aatcaccccc
     6181 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     6241 ccaaagcctg gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     6301 cgcgccgggc cagttccgcc cgtggtaagc accagatggc aactcatttc atccaccagc
     6361 tcacacaacg tttgctcgat gatcgcctgc tcatcgggaa ttaagcgggt ttccagttca
     6421 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg cagggatgcc tttatcctga
     6481 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     6541 aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca
     6601 gacaggtttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     6661 agatcgccga tcattttttc cagtttttcc tggtcaacag caaacttacg gataccttcc
     6721 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     6781 atacgcgccg gacgcgcttt cacttcacca gtgtaagaca gtttacgttc gatagcccct
     6841 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc gcagcctgcc
     6901 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     6961 tgctctttgt agtactggta gatttcagat acagaaacca cgcctggatc ttctgccgga
     7021 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     7081 gagatcagga acacgcccgc ttccgcacaa gcacgagcct gagcgaagga gaacagcagg
     7141 gtcaggttac agttgatgcc ttctttttcc agctgttccg cagcacggat accctgccag
     7201 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
     7261 atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact
     7321 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tacggccagt
     7381 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
     7441 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
     7501 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
     7561 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
     7621 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
     7681 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
     7741 gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca
     7801 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
     7861 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
     7921 ttggggttcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
     7981 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
     8041 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cagcgcgcgt
     8101 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac ctggagccgt
     8161 cttctggatg tgggttgccg catttatcgg catggcgacc tcgtttgccg aatgttccct
     8221 cgcacagctt tataaagaac gtgatgccaa tgggcagttt cgtggcggac cggcatggta
     8281 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
     8341 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcgcgag ccctgagttt
     8401 ttcttttgat tttcccccgc tggtgacagg tattattctc gctgtctttg ctatgctggc
     8461 gatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
     8521 gataatctgg gtactgacca gcctggtgat ttgcgtaatg aatatcgggc aacttcccca
     8581 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
     8641 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
     8701 ggcgggaatg gggtcgacgc caaacgcggc agcggcagct gcgtcctggc ctccgcatcc
     8761 ggcagcgcaa ggaattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
     8821 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
     8881 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctggggtg cggagtttgt
     8941 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
     9001 aaacaatctc ttctttttac gcctgaacac ccctaaagcg atctggtgtc tgcggatctg
     9061 caccttcgca acggtcatcg gcggcacctt gctaagtctt ccgctgatgt ggcaactggc
     9121 agatatcatt atggcctgca tggctattac caatttgacc gccatattac tgctctcgcc
     9181 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
     9241 gtttgatccg ttgcgttatc cggagatcgg tcgccagctt tctccggacg cgtgggatga
     9301 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
     9361 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
     9421 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaactccca
     9481 gcagctgatc catgaagcgc ggaaactgac gcccccgcag attagctccc tgatgcgcat
     9541 cagcgataaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
     9601 accggaaaat gcccgccagg caattctggc gtttaaaggt gatgtctaca ctggcttgca
     9661 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
     9721 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
     9781 gatacgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
     9841 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtagtgataa atctggcgtc
     9901 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccagt
     9961 gttcctcgat gagaagaacg gcaaatttaa gataatcagc ttctacgcta agaaagcacg
    10021 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactaactgg
    10081 ttttaatagc gaaggttact tctttgatga agcttcctcc agcaatggcg aactggtgtt
    10141 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atggcaatcc ggcattgata
    10201 tttagcgatg atgtttgtca ggccccggac gatgatcgcc acgatgatca ttgtgcttgt
    10261 tatggcgcgg cgatggcggc ggtccatatg ggtgccagcg attgcctcgc cattcataat
    10321 gttgtttcca ccagccgtgg tcgcgccagt gaccgccatc ccagtaatag ccacgattat
    10381 cacgatcgcc tatctgtaat tttactgacg ggactagcgt aatttccgca gcctgcgctg
    10441 ccatgggagc gaacagaacc agggaaagtg cgagtacgat agattgcatc tttttcacag
    10501 gttactcctt ttatcattgc ccgttatggg tcgatatccg gagattacgc gatgagaacg
    10561 gcagttgtta ttctccgcaa tcctaatctc taagttaccg catttattga gaatttctcc
    10621 tttttccctg tcgtttttct ccatattttc ttcataaaaa agccgggctg cataaaagca
    10681 aacccggcct gattgagata atgaatagat gttactgatg attcatcatc aatttacgca
    10741 acgcagcaaa atcggcgggc aggttatgcg aaagcaaggg taaatcagca cgttctgcca
    10801 gctcttttgg cagatccaac gtttcaccga gaatcgcttc cacgctctct ttaaattttg
    10861 ccggatgcgc ggtgccgagg aacaagccat attcgcctgg attcaactgg tcacgcagcg
    10921 cacgataagc tacggcagcg tgcggctccg aggtatagcc cagttctttt aactcacgca
    10981 ttgtctgttg cgtggtttca tcatccacgg ctgcataccc cagctctttc agttgccaga
    11041 ttttgcggcg gaacaactct tccacacgcg gccagttgtt tggctggcta acatccatcg
    11101 cattggataa cgtcgcctga gtcgctttgg gtgaccactg accgccgtgc aggaaacgtg
    11161 gcacggtatc attcacgttg gtcgcagcaa taaaacgctt caccggcagg ccaagagatt
    11221 tcgccagcag gcccgctgtc aaatcgccga agttaccgct cggcaccgag acaaccagct
    11281 ggttgcgcgc ttcctgcggc aactgcgcca cggcttcaaa gtagtagcaa atctgcgcca
    11341 gcaaacggct gatgttaatg gagttagcag agtttagccc cagcgccact ttcagctctt
    11401 catcatcaaa cgcctgcttc accagcgcct gacaggcatc gaaatcgccg tcgatggcaa
    11461 cagtttcgat attgccgccc aatgtacaga acagtttttc ttgcagtgga ctgattttgc
    11521 ctcgtggata gaggataacc actttcacat tcggtaaacc gtagaaagca tgagccactg
    11581 ccgctccagt atcaccggat gtcgcggtca gaatggtcac tggcttatcg cccgcaatat
    11641 gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt
    11701 ggaacaattc cagacaaccg acatcgcttt caacattggc gaccggagcc gggaaggcaa
    11761 acgccgcacg tacgcgctct tccaggattt cctgcgggat ttcatcacca ataaacgccg
    11821 agaggatctt cgcactgcgg gtgacaaaat ccagcttcag catctcatca atttcagtca
    11881 ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg atttttgcct aacccctggg
    11941 ttacggcttg cgcaaagctg acctgctcat tgtgatcttt cagattgtag agtttcattt
    12001 agttttccag tactcgtgcg cccgccgtat ccagctggca aatatgaaca aaaccttcct
    12061 gattttgcag gtagttctta cccaaccagt cggcaacgcg ctgagcggta tccggcttgt
    12121 cacacagagc gaacaaggtc gggccggagc cggagatacc gctcgcaacc gcgccgattt
    12181 ccgcaaccgc ctgccgcgcc tgccggaagc caggcaataa ccgttcacgg tagggttcag
    12241 cgataacatc tttcatcagc ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa
    12301 tgaatcctgc caaatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta
    12361 aaatagcccg ggcttctgcc gtcgagactt taatccccgg atacgccagc acccacagcc
    12421 actcatcaaa ccctggcact tgctgactga tgatgtcgtt ttcttcaatc atcaactgca
    12481 taccaccaag aaaacacggt gccacgttgt cgtaatgaat gctgccggag atacgccctt
    12541 ccaactcgcc catcaaagcc agcaaacgag tgtcattaag cggcttgccg cagtgttcat
    12601 tcatcgccat cagcgccgcg accactgaac aggcgctgga gcctaagccc gaaccgatcg
    12661 gcatattctt ttccagagtc atcgccactg gaatttgctt gccaagctcc tggcaaaaac
    12721 gctcccagca ctgataaacg atattttccc gtggctctga cggcagctta tcggcaaagc
    12781 gtccgaggtt gttgagactg aatgtctctg ctgcctcaac cgtgactaca tctccgagca
    12841 atgcaccatc aacaggtgtc accgccgccc cgagcacatc aaacccgacg ctcatattgg
    12901 cactggaagc cggggcataa actttaacca tgtcagactc ctaacttcca tgagagggta
    12961 cgtagcagat cagcaaagac accggcagct gtaacgtcat tgcccgcacc atatccgcgc
    13021 agtaccaacg gcagcggctg ataatagtgg ctatagaagg ccagggcgtt ttcgccattt
    13081 ttcactttga acagcggatc attaccatcc acttcggcaa tcttcacgcg gcagacgcca
    13141 tcttcatcaa tattgccaac atagcgcaaa acttttcctt catcacgagc cttcgccaca
    13201 cgcgcggcaa agagatcgtc gagctgtgac agattcgcca taaaagcggc aacatcaccc
    13261 tcggcgttaa actctgcggg cagcacaggt tcaatttcaa tatccgccaa ctccagttca
    13321 cgtcccgttt cacgagcgag aatcaatagt ttacgcgcca catccatacc agaaagatca
    13381 tctcgcgggt ccggttcggt ataacccatt tcccgcgcca gtgtggtcgc ctcggagaaa
    13441 ctcatgcctt cgtctaactt gccgaagata taagaaagcg aacctgaaag aatgccggag
    13501 aacttcatca attcatcacc agcattgagc agattttgca ggttctcaat aaccggtaat
    13561 ccagccccaa cgttggtgtc atagaggaat ttacgccgcg atttttccgc cgcataacgc
    13621 aactgatggt agtaatccat cgacgaggtg ttggcctttt tgttcggcgt aacaacgtgg
    13681 aaaccttcgc gcaggaagtc ggcatattga tctgccacag cctggctgga agtacagtca
    13741 acaatgaccg ggttcagcag atgatattct ttcacgaggc gaattaagcg cccgagatta
    13801 aacggctctt tggcttgcgc cagttcttcc tgccagtttt ccagattaag gccatgtaca
    13861 ttggtgagca gtgccttcga gttagcaaca ccgcagacac gtaagtcgat atgtttattc
    13921 ttcaaccagc tttgctgacg cttcagttgc tccagcagcg caccgccaac gccaccgacg
    13981 ccaatcacaa acacttcgat aacctgatcg gtattgaaca gcatctgatg agtaacgcgc
    14041 acgccagtgg tcgcatcatc gttattgacc acgacagaga ttgagcgttc agaagatccc
    14101 tgagcaatgg cgacaatatt gatgttggcg cgggccagcg cggcaaagaa tttcgccgag
    14161 atcccacgta aggtgcgcat accatcacct accaccgaga taatggccag ccgttccgtc
    14221 accgccaacg gctccagtaa gccttctttc agttccaggt agaactcttc ctgcattgcc
    14281 cgttcagctc gcacacagtc gctttgcgga acgcagaaac tgatactgta ttcggaagat
    14341 gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca tcgctgcaaa gacgcgcgcc
    14401 gccatgccaa ccatcccttt catccccggg ccggaaacgc tgaacattgc catgttattc
    14461 agattggaaa tgcccttgac cggtaattcg tcttcatcac ggctggcacc aatgagcgta
    14521 cctggtgctt gaggatttcc ggtattttta atcaggcaag ggatctggaa ctgggcgatg
    14581 ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt aagaaagctc catcgcttcc
    14641 tgataggaca tcgacttcaa caacctcgca tcgggcacct gacgcggatc gcaggtataa
    14701 acaccgtcaa catccgtcca gatctcgcaa caatcggcgc gtaaacaggc cgccagcacc
    14761 gcagcggagt agtcggaacc gttgcgtccc agaaccacca gctcgccttt ttcattaccg
    14821 gcagtgaaac cagccatcag caccatgtgg tcagccggaa tgcggcttgc cgcaatacgg
    14881 cgggtggatt cagcaatatc aacggtagat tcgaggtaat gacccactgc cagcagtttt
    14941 tcgaccggat cgataacggt aacgttgtga ccacgcgctt ctaacacgcc ggccataatg
    15001 gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc cgggcactgc
    15061 cccaacaaac tgatgccatg caggacatgt tttatttggg caaattcctg gtcgacgaaa
    15121 gttttcaatt gtgccagcgg aaatcccggc tgggcggcgg cgagtcccgt cagaagttcg
    15181 gcaaaaatac gttcggcatc gctgatattg ggtaaagcat cctggccgct aatagttttt
    15241 tcaatcatcg ctaccagatg gttggtaatt ttggcggggg cagagaggac ggtcgccacc
    15301 tgcccctgcc tggcattgct ttccagaata tcggcaaccc gcagaaaacg ttctgcattt
    15361 gccactgatg taccgccgaa cttcaacact cgcatggttg ttacctcgtt acctttggtc
    15421 gaaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag
    15481 cccgcaccgt tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc
    15541 atggatgttg tgtactctgt aatttttatc tg
//
LOCUS       DACADA010000107        15560 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.109, whole genome shotgun sequence.
ACCESSION   DACADA010000107 DACADA010000000
VERSION     DACADA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.109"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(34..3915)
                     /gene="entF"
                     /locus_tag="ISB17_003287"
     CDS             complement(34..3915)
                     /gene="entF"
                     /locus_tag="ISB17_003287"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAP1981186.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            complement(3912..4130)
                     /locus_tag="ISB17_003288"
     CDS             complement(3912..4130)
                     /locus_tag="ISB17_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAP1981187.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4133..5335)
                     /gene="fes"
                     /locus_tag="ISB17_003289"
     CDS             complement(4133..5335)
                     /gene="fes"
                     /locus_tag="ISB17_003289"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAP1981188.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     AIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTIGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5578..7818
                     /gene="fepA"
                     /locus_tag="ISB17_003290"
     CDS             5578..7818
                     /gene="fepA"
                     /locus_tag="ISB17_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAP1981189.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7993..8652
                     /gene="entD"
                     /locus_tag="ISB17_003291"
     CDS             7993..8652
                     /gene="entD"
                     /locus_tag="ISB17_003291"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAP1981190.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPTGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDAGFLRYQIRNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(8654..9589)
                     /locus_tag="ISB17_003292"
     CDS             complement(8654..9589)
                     /locus_tag="ISB17_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAP1981191.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGITLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(9582..10412)
                     /locus_tag="ISB17_003293"
     CDS             complement(9582..10412)
                     /locus_tag="ISB17_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1981192.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
     gene            10775..11725
                     /locus_tag="ISB17_003294"
     CDS             10775..11725
                     /locus_tag="ISB17_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAP1981193.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            12076..13596
                     /locus_tag="ISB17_003295"
     CDS             12076..13596
                     /locus_tag="ISB17_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAP1981194.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            13700..14635
                     /locus_tag="ISB17_003296"
     CDS             13700..14635
                     /locus_tag="ISB17_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1981195.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            14682..15302
                     /locus_tag="ISB17_003297"
     CDS             14682..15302
                     /locus_tag="ISB17_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HAP1981196.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGSITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            15304..>15560
                     /locus_tag="ISB17_003298"
     CDS             15304..>15560
                     /locus_tag="ISB17_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP1981197.1"
                     /translation="MLPQEIAPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNST"
BASE COUNT         3878 a         4064 c         4020 g         3598 t
ORIGIN      
        1 tgaggttatg gcaagttctt tactaacgta cggttagtta ttaatttgtg tatttattag
       61 tggtcctatt tctttaaaat actccgggga aataatatcc acatgcgcac aatcctgacg
      121 atagatatcc agctcagcaa tccacggtgc ccaggcctgt tcaggactca taccttcctg
      181 aagcgtgcgt tcggcaacaa atagcgtcgc tttaccatca aacgctacgc tatgagctgt
      241 cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
      301 agtacttccc tgctgtgccg ccaggaaggc ttcgcgctcg cggttaatct ccgccagcac
      361 ttccgggtcc agaccattgg cttctttttc ctgccagttt tgcgtttctg gcggccaggt
      421 atccagcaag ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatgcc
      481 ctgcgccagc gtaccgccaa gtgaataacc cagcaggtaa taagggccgt gcggttgttg
      541 ttcaaataac gttgccagat gcgcttcgca gacttcatcc aggtttgccg ccgtctgcat
      601 ggggccatga gggcgcggcg actgaatgcc gataatcgac cattgtggat cgagataacg
      661 cgagagcacg ctgaactgcc aggcaaaacc ggatgccgga tggaaacaaa acagcgtcgg
      721 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgtcggg tgctgtcttc
      781 ttcaccatca ataatcgttg ccagtttggc gacagttgac gcgaccatca cctgccccgg
      841 cgtcacctga cgggcaaact gtcgacttaa ctgcgctgcc agtttcattg ccagtagcga
      901 atgaccgcca agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
      961 cgagaatgcc gcggcgataa tcgtttcact gcccgctttc ggcacacgcc ccggcgtttg
     1021 tgccttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagtgg
     1081 taactgtgga agttgcagca gaaccaccgg taccatatgt ggtggcaatg tttctcgaag
     1141 ctgcgcctgt aatgcgctgg tatccaacgg cagacctgat tgcgacacca gatagcccac
     1201 caactgacgc gcatcaccac cagtggctgc cgcctggtta atcacacagg cgtgggtaac
     1261 ggcttgttcg acatccggca gcgcctgcat cacgcgatca atttcgccca gttcgatacg
     1321 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccactg cgccgttatc
     1381 cagccagcgg gcaacgtctc cggtacggta catccgttca cctggggcaa aaggatcggc
     1441 aataaagcgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttgaatacc
     1501 ggtgagatag agatcgcccg ccacacccgg cggcaccgga tgcatcatcg catcgagaat
     1561 gcgcaggccc gtattccaca ccggataacc aatcggcaca ctgctgccgc gcacctctgc
     1621 cagttcctcg ccaaaagccg gataccaact gacatctacc gccgcttccg tcgggccata
     1681 tagattatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat cagccggtag
     1741 ggcttcacca ctacagaaaa cctgtttcaa cgtcgagcaa ttctgacgag cggtttgcgg
     1801 cgtcagcgat gcaacaaatg ctgccagcat cgacggcaca aagtgcgtgg tcgttacacc
     1861 atattcggca aagaattgct gcatagcgag cgggtcgcgg tgcgcttccg gttcagccat
     1921 caccagttta gccccggcaa taaacggcca gaaaaactcc cacaccgaga catcaaaact
     1981 gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtggt tttgcatcca
     2041 aagcaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
     2101 gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagctgca gcggcgcact
     2161 gccctgcggt gtaagcgggg cgttatagca aaggcgggtt aaatctggaa catcggcaaa
     2221 gcgcggcagt tgatcgtcag tggtgattaa cagcgacgga cgcgcatctt ccagcatcat
     2281 tttcaggcga tcgtccggat agccggtatc cagcggtaac caggccgcac cagcctcaac
     2341 aattgcatgt agcgcgagag tcaaaaagac cgagcgcggt agagccaccg ccacgctgtc
     2401 gcccggttta acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
     2461 ttcccgatag ctgaactggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
     2521 cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaatct gagtggcgtt
     2581 gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag
     2641 cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcgt gttgaattaa
     2701 cgttggctca tcgtaacgct gtttattggc gaggatttca atactcaaat ctccgtgttc
     2761 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg gatgggtttg
     2821 cgcctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc
     2881 gaacagcggt tcctctcctg ccgctcgccc actgtcacgg acaatttgtt cggcgtcgta
     2941 gcgttgatgg cggcgcattt ttttcagttg tgctgccagt cgggttgcca gttgcggcag
     3001 cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg gtccggtagc
     3061 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcagcgt agtccatgcg
     3121 gttgcacaat cgccccagcc acaaggctgc caacgcaagt gctaaatcgg tacgctgcac
     3181 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
     3241 caaaatatct gccgaagcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg
     3301 tggcagttga cgacgctgtt ccgcccagaa tgccgcatca cgctgccagg cttcactttc
     3361 gcggtactgc tgatactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt
     3421 tggttcgcca cgcagcaatg cgcagtaaat attggcgatc tggcgtgtaa tggccgggaa
     3481 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc
     3541 cacctgtatc agctgatgaa agaccagtgg tttaccacta tcgacgcgca gatcttgttg
     3601 caaatccgcc tgcattaatg cccgcgcagt accgtgcgga tcaatgttgg ttcgcaggtc
     3661 gataatttct ggcagttcga acgtctgcgc atcatcgacc cactgccaga cttcgccgtt
     3721 atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccggcaa ccaccgcgcg
     3781 ggccagtagt ggcgcatcaa cctctccggt taactcaacg taatgcgcca cgctccaggc
     3841 ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
     3901 taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tcgccggtgt cagagtacgc
     3961 cagtgggctt ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaacgtcc
     4021 cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc
     4081 aaaatgtaaa acgctccctg tggatcatcg aagggattac tgaatgccat attcaactcc
     4141 tgtcatggaa aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac
     4201 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt agggggtgta
     4261 attgcgcata cagcgcctga ttggcctgca tgatcatcgg ctcgcgaaca cccgcttcca
     4321 gcacaatgcg cagaccttcg gcgctaactt caccagtttt tagctgttca agtaacatgc
     4381 cctcttggtg cccgccccga tgcggccacc agtacgaacc tgactggctt aatacacagc
     4441 caaagcgttc aggccagtgc agtccggcat acagcgcgga aagcccacca aaactctgtc
     4501 cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc acctggggta
     4561 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
     4621 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg cggaagttgc cgacgatggg
     4681 tcagcgaagt cagcgcaggc cagacgggca tactctgcgc ccaaaattcg ccatcaagca
     4741 aaactgccag cgggcgttct tctgctgttg catcgccgat ggtaaaaatc catacacggc
     4801 gtgaattttt caaccgttca cttttccaga taatttcttt ggcaggcgtt tctggcgctt
     4861 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
     4921 gccctcgccc gcctttccag ctttgtggat tcagcggatc ggctatcgcc tggggtaata
     4981 gttttcgcca gccttcgcgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat
     5041 cgcgttcggt aggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc
     5101 actgccaaac atcggttcct gcaattcgct gcatcgaccg gggctggctg ttctgatggt
     5161 gatcggtcat gccagtgata tagacccata cgcgctttat cgccgagtgg ccttcagaac
     5221 cttgtggatc acgccaccaa aaagtgactt caaacatttc gtcattcaga cgttgccatt
     5281 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
     5341 aaccccatgt ttactgtgca attttttatt gattgcagaa atatattgat aatattattg
     5401 ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta
     5461 atcagctgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
     5521 ttgatgacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
     5581 aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcacag
     5641 gcacaagagc cgaccgatac tcctgtttca catgacgata ctattgttgt taccgccgcc
     5701 gagcagaact tgcaggcgcc cggcgtttcg accattaccg cagatgaaat ccgcaaaaac
     5761 ccggttgccc gcgatgtatc ggagatcatt cgtaccatgc ctggcgttaa cctgaccggt
     5821 aattccacca gtggtcagcg tggtaataac cgccagattg atattcgcgg catgggtccg
     5881 gaaaacacgc tgattttgat tgacggcaag ccggtaagca gccgtaactc tgtgcgtcag
     5941 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccgcc tgaaatgatt
     6001 gaacgtattg aagttctacg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc
     6061 ggcgtggtta acatcattac caaaaaaggc agcggtgagt ggcacggctc ctgggatgct
     6121 tatttcaatg cgccagaaca taaagaggaa ggtgccacca aacgcaccaa cttcagcctg
     6181 accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag
     6241 gctgacgcgt gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg
     6301 acgttaccag ccgggcgcga aggggtgatc aataaagata ttaacggcgt ggtgcgctgg
     6361 gacttcgcgc ctctgcaatc actcgaactg gaagcgggct acagccgcca gggtaacctg
     6421 tatgcgggtg atacgcagaa caccaactct gacgcttaca ctcgctcgaa atatggcgat
     6481 gaaaccaacc gcctgtatcg ccagaactac tcgctgacct ggaacggcgg ctgggataac
     6541 ggcgtgacca ccagcaactg ggtgcagtac gaacacaccc gtaactcgcg tattccggaa
     6601 ggtctggcgg gcggtaccga agggaaattt aacgaaaaag cggcacagga ttttgtcgat
     6661 atcgatcttg atgacgtgat gctgcacagc gaagttaacc tgccgattga tttcctcgta
     6721 aaccaaacgc tgacgctggg tacagagtgg aatcagcaac ggatgaagga cttgagttcc
     6781 aacacccagg cactcaccgg aacgaatacc ggcggtgcta ttgatggtgt gagtgccacc
     6841 gaccgtagcc catattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caatatggag
     6901 ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc
     6961 aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa
     7021 atgggcatcg cccgcgccta taaagcgccg agcctgtacc agactaaccc aaactacatt
     7081 ctctacagta aaggtcaggg ctgctatgcc agcgcgggcg gctgctatct gcaaggtaac
     7141 gatgacctga aagcagaaac cagcatcaac aaggagattg gtctggagtt caaacgcgac
     7201 ggttggctgg cgggcgtgac ctggttccgt aacgattatc gcaataagat tgaagcgggc
     7261 tatgtagctg tagggaaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg
     7321 aaagcggtgg ttgaaggtct ggaaggatcg ttaaacgtac cggttagcga aacggtgatg
     7381 tggaccaata acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg
     7441 tcgatcatcc cggagtatac gttgaactca acgctgagct ggcaggcacg ggaagatttg
     7501 tcgatgcaaa cgaccttcac ctggtacggc aaacagcagc cgaagaagta caactataaa
     7561 ggtcagccag cggttggacc ggaaaccaaa gaaatcagtc cgtacagcat tgttggcctg
     7621 agcgcgacct gggatgtgac caagaatgtc agtctgaccg gcggcgtgga caacctgttc
     7681 gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc aggagccaac
     7741 tatatcgcag gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtatatgagc
     7801 attaatactc acttctgaag ctaacgtcag attgttgaca aagtgcgcgt agttcatgcc
     7861 ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag
     7921 gaccacgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc atcattctct
     7981 aatatggttg acatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat
     8041 tttgttgagt tcgatccggc gagttttcgc gagcaggatt tactctggct gccgcactac
     8101 gcacaacttc aacacgctgg acgtaaacgt aaaacagagc atttagccgg gcggatcgct
     8161 gcaatttatg cgctgcggga atatggctat aaatgtgtgc ctgcaatcgg cgagctacgc
     8221 caacctgtgt ggcctacggg ggtatacggc agcattagtc actgtgggac tacggcatta
     8281 gccgtggtat ctcgtcaacc gattggcatt gatatcgaag agattttctc tgtacaaacc
     8341 gcaagagaat tgacagacaa cattattaca ccagcagaac acgagcgact cgcagaatgc
     8401 ggtttaacct tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag
     8461 gcatgtgaaa gacaaactga tgcaggattt ctgcgctacc aaatcagaaa ttgtcagcat
     8521 caacagatga atataagcgg gccgaatggc gaccatatcg tgcagtggtt agtaaggggt
     8581 aataatgtta ttaccttgtg cctggcagat aaacacccgc acactgcagc atgcgggtta
     8641 tctggcgggt aattcaaagc atttcccgtg cctgagctgc aattccggca gcatcaaatc
     8701 catgatgctg acgcaaccac ccacgatccg ccgctgcggc atattctcca tccataatgc
     8761 ctgcgcgttt taatgtgatc cccgttccgg cctctgccaa cacttccgcg accaggctac
     8821 caaggccgcc attgacgtta tgctcttcaa ccgtcatcac tgctttgcat ccttttaacg
     8881 ctgccagcaa tgctttggtg tcgcatgggc gaatcgacgg tacgctgaca actgtagccg
     8941 cgataccaga ctgcgctaaa agttctgcgg catcgaccac ttcatgaacg gtagagcctg
     9001 tggctaccag cgccagttcg ctgccctcgc gcaaagtcac cacggcacct ggtacaaatc
     9061 ggtagtcctc atcgtggatt tccggcaggg ctttaccgtc catacggata taaacaggcc
     9121 cctgatagcg aagagcataa tcaatcacct gacggcactc ccgcggtgaa gacggggcaa
     9181 agatttgaat attgccgaaa ccacgcataa ccgcaatgtc atcaatcgca tgatgcgtgc
     9241 tcgccagcgg tccataactc gccccggcat tcagaccgaa caattttacg ttggtaaagt
     9301 tgtagcagac atcaaccttg atttgctcgt tagcgcgagc gataagaaac ggcgcggcat
     9361 tgcaggtaac agccaccttg ccgcccagcg ccagcccggc agctgtacca accatgctct
     9421 gttcggcaat acccacattc accagtcggt cagggaattg cttaataaag ggagcaattt
     9481 ttgccgtaga cgttgaatca gccaccacag gcaccaaatc gatcccctca tttacggcat
     9541 cgataaacgc ctgaaccatc acatttgcca gatgttcact gttacgcatc tttgagttcc
     9601 tccagtgcct gttcaatttc atcgcctttc ggcacccggt gatgccattc cgcacgccct
     9661 tgtataaagg aaatgcctgc ccctttagtg gtgtgggcaa tcaccacatt cggtttgcct
     9721 tcctgtttaa gctcttcaag cgtcgcgaca accgaagcca tatcattgcc ctgacattca
     9781 gtaacctgca taccgaatgc cagccatttc tctgccagag gatcggtgtt catgatttcg
     9841 cgcgttggcc cggctaattg cagattattt ttgtcattaa taatgaccag gttatcaagc
     9901 ccgtaatgcg ccgccgccag tgcagcttcc cagttactgc cctcagccag ctcaccatcg
     9961 ccagtaatta agaagatacg acgacggctt tgactttttt tcgccgccag cgcaagtcct
    10021 acagccacag ggaaaccatg ccccagtgct ccggtattca gttcaatacc tggcgttttt
    10081 tgccgaaccg gatggccggg taagtgtgaa tttggctgct gataagtgga aagccattcc
    10141 agtgggaaga atcctgcttc cgccagcacg cagtagtaac atccaacagc atggcctttt
    10201 gactgaatgt atatatcccg gtcatcgtcg tgaaggcgat ccggtgcgac attgagaata
    10261 cgaaaataga gtgcggtcag taattcaacc tgagagagat cggccccggt gtgtccacct
    10321 gccgggctat tggcatttaa gcgcacgata tggcggcgta ccgcccgcgc tttacgctca
    10381 agggtctgga tatcatagct atagggattc atttaatacc tctgaattta ttttcaacat
    10441 ggaatattta ttccaccggg gactaaaaaa accagaaatc ccagggcgtt gcgcggcaag
    10501 acttacgctg ctgcatcata ctcaggaaca ttctgcctcc ttacaccgtc agttacatgt
    10561 ccgggtaatc atcagttgac ctgatatcat cttgaatata tattcaataa tgattcttta
    10621 ttcaattatg ccgcctgttt tttatttttg tccatcgctg attttaagga ttggttaaag
    10681 ttagaactcc atcacactta actgtcttct tttttcgcgt atgataaaag cctctaagat
    10741 aagggcggta cgttaatgat ttacggggaa atatatggct aagcaggatg aacagcggct
    10801 actggtgaag atcgccactc tttactatct tgaggggcga aaacagtctg acatcgccca
    10861 acttctctcg ctttctcagt catttatatc cagagcgcta acccgttgcc agaaagaagg
    10921 cgtggttaaa atcagcgttg tccagccgtc aaatatcttt ctcaatctgg aaaaaggcat
    10981 tgaagagcgt tacggcatta aacaagcgat tgttgtcgat accgaagatg atgccagtga
    11041 tcacaccatt aaacgcgcca tcggctctgc cgccgcgcac tatctggaaa cgcgtttaag
    11101 gccaaaagat ttcattggcg tctcttcgtg gagttctacg attcgcgcga tggtcgatga
    11161 agttcacgcc caaaacttaa aagccagcgg cgttattcag ctgctgggcg gcgtggggcc
    11221 aaacggcaat gtgcaggcaa ctattttgac acagaccctg gcccagcatc tgaattgcga
    11281 agcctggttg cttccttctc aaagcattga aggttcggta gaagagaaaa aacgcctggt
    11341 ggcaagccag gatgtggctg acgttatcgc cagatttgac gacgtcgata tcgccattgt
    11401 cggcatcggt atccttgaac cctcgcagtt gctaaaaacc tcgggcaact attatcacga
    11461 ggatatgtta caggtactag cggatcgcgg tgctgtgggc gatatctgcc tgcactacta
    11521 cgataaacat ggtcagcccg tgttgcagga cgatgaagat ccagtgattg gtatggcact
    11581 ggataaaatc aaaaaatgcc cgaatgtggt ggcactggcg ggcggcaaag acaaagttgc
    11641 cgccatcaaa ggcgccatgc agggtggtta tattgatgtt ttgatcactg atttccccac
    11701 cgccagaatg ctgattgcgg attaatcctc tcctcccttt cttctgcccg cctttgcgaa
    11761 aaaatcttct cgcaaaggtg attgcttaaa aagcctttaa tatcaataaa ctgaaaggat
    11821 tcaacaatcc tgtttctcga ggctttctca ccctgtcacc acgcttctaa gtgtgatagc
    11881 cagcacattt gatgattttt tctctagctc aggaagaata atctggctaa ggtataaata
    11941 aataatcagt atcgaatata tattcatgcc ttaacacaaa atgtcacagc agggcctgat
    12001 gaagtaataa cgtgaccatg aggaacgcct catctccttc cacgccagga tgcaatgtga
    12061 ctcaggagac tacctatgtt tgcaggaaat aaagagttcg ttattgcgtt ggatgagggt
    12121 accaccaatg caaaagccat cgcacttgat ggtttcggta acgtggtggc gaagttttct
    12181 caaccactgg ctattcagac gccacgtgaa ggctgggttg agcaatccgg ggaagcgtta
    12241 atctctgcct cgcttgatgt tattgcccag gccatcaacc acgtgggggc agaaaatgta
    12301 gcggctctgg ctatcagtaa tcagcgtgaa acggctatcg gctggtatcg tcagagtgga
    12361 aaaccactca atgcggcgat tacctggcaa tgcacgcgca gtgccgagtt ttgtgaaaca
    12421 ctgcgtcgcg agggcaaaga gcagcaaatt aaaacgacta cgggtcttcc tatcgccccg
    12481 ctattttccg cgtccaagat gcgttggcta ctggaatcta cacctgaggg tgtgcaactt
    12541 gccgaccagg gtgaaatttg tctcggcacc attgatgcct ggttgctatg gaacttgact
    12601 gctggcgatt cattttgctg cgactactct aacgccgcgc gaactcaatt acttaacctg
    12661 aaaacaggcg agtgggatga ggaaatgctg gcgttgtttc acattccacg cgctgcattg
    12721 ccagaaatta aaccttccag tggcctgttc ggttataccc gagggcttaa aacaattccg
    12781 gatggtattc ccgtcatggc gatggtgggc gattctcacg ccgctctttt tggtcatgcg
    12841 ttgggagaag cgggatgtgt gaaagcgacc tatgggaccg gttcttccgt catggccccc
    12901 gtcaactctg ctcagtgtga tatcgccaca cttgccacaa ccattgcctg gcatgatggc
    12961 gaaaatatta tttacggtct ggaaggaaat atcccccaca ccggcgatgc cgtggcatgg
    13021 atggctgaca gcaccggttt aagtgaactg acagacgcgg agctggccct cgaactgaat
    13081 accctgccag cctcagtgga ttcgactatg ggtgtttatt ttgttcctgc tctcactggc
    13141 ctgggcgcgc catggtggga tgacagcgct cgcggcgtgg tgtgtggctt aagccgtggc
    13201 gtaaagcgcg cgcatttgat tcgtgccgca ctggaatcaa tcacttatca aattgcagat
    13261 gtagtggagg caatgcaaca gcatgatgat tttcacttat cggcgttaat ggtggatggc
    13321 ggcccgacca aaaatgactg gttgatgcaa tatcaggcgg atctgctggg ttgcccggtg
    13381 atgtgcagtg atgtgccaga gctttcagcc attggtgcgg gcctgctcgc ccgcaaagcg
    13441 cttatccccg gtaccgttgc agatttaaaa gcgttattaa ctgaacacag tgaatttaaa
    13501 ccggacatgg cgcgtcatca gcgactgcaa aagagatggc aggagtggcg tcatgcggta
    13561 aacagaacat tatggaaacc ggcatcgtca atttgacgaa caagactaac caacgtaagg
    13621 agaataccgg cccataaata attcaggctt tacgttgttt caccgtaccc gacaataaca
    13681 acacagagag aacattgtca tgaaattacg tttaacactg ttaaccgctg cgaccctgac
    13741 tgctctttct ttttctgctc atgcggtaga aaaaggaacc attatgatta tggtcaattc
    13801 actggataat ccttattacg cctcggaagc gaaaggtgcc agtgaaaaag cccagcaact
    13861 gggatataaa acgaccattc tttctcatgg tgaagatgtc aaaaaacaga atgaactgat
    13921 cgatactgca attggtaaaa aagttcaggg cattatttta gataatgccg actccactgc
    13981 cagtgttgca gccattgaaa aagcaaaaaa agcaggtatt cccgttatat taattaatcg
    14041 cgaaatacct gtggatgatg ttgcactggt gcaaattacc cataataact tccaggctgg
    14101 ctcggaagtt gccaacgcat ttgtcgagaa aatgggagag aaaggaaaat atgccgaact
    14161 cacctgtaat cttgctgata ataattgcgt aactcgctcg aaatctttcc accaggttat
    14221 tgaccagttc cctgaaatgg tcagcgtggc gaagcaagat gccaaaggca cgttaattga
    14281 tggcaaacga ataatggaca gtattctgca agcccatccc gatgttaaag gggtgatttg
    14341 tggtaatggt ccggttgcac tgggagctat cgcagcactg aaagccgcca atcgcaatga
    14401 cgttattgtt gtcggcattg atggcagtaa cgatgagcgc gatgccgtga aagccgaatc
    14461 attgcaggct accgtgatgt tacaagcaca agccattgcc gcgcagggtg ttaccgatct
    14521 ggataattac ctgcaaaaag gagttaaacc agccaaacag cgggtgatgt tccgcggcat
    14581 tctgataacc aaagataacg cagataaagt ccaggatttc aatatcaaat cctgatccca
    14641 ggtcgttgcg ccctcacaat gagggcgcag aggagaatat tatggataaa aaagtctggc
    14701 tggcgctggc aattctgagc ctgggtggat gtcgcatcgt ctcgcagcag gaacttgccg
    14761 atctcaaatc cccgcctaat cctcatatgg caaatatcga tcagacctgg cagaaaaaca
    14821 tcgtgccgca ggtggtagaa aatgcgcgtc cggttgcgga gttaatggcg gcgctacagg
    14881 cagaaaaaga tgtcgatgct gcctgtaaaa cgctgggcta tcgttctcag gaagaaaacc
    14941 cgtgtatttt ctacgttaag gtcgaaggta gcatcaccaa tattgatgcg aaatcccgta
    15001 gcggaaaaat gaccatcaca gatatcagcg gcacaaatat tgtcgtgcag acggggccaa
    15061 ctcttcgcgg cacgcttctg cgagatgcat ataaaggtgc aagctatgag cattttaacg
    15121 atcaggtttt atttggtgaa tatggtcgtg ctatcaatga gcaagccatc aatatgattc
    15181 agtcggcaaa gcttaaaacc ggggaaacgg ttcaggttta tggcgtattt tctgcatggg
    15241 atattccgca aactatcccg gatataacgc ctgcgaaaat agccagggcg ggaggcgagt
    15301 aatatgttgc cgcaagaaat tgcccccccc cagactgatg aatcacctgt aatcatagaa
    15361 acgcatgact tatcgcgtat ttatccgggt gtggtggcgt tagataacgt gaattaccga
    15421 gtctatcgca ataaagtaaa tgtgcttatt ggtgaaaacg gtgcgggtaa atccacaatg
    15481 atgaaaatgc tggcgggggt tgaaacacct tcctcaggca agatcattct tgatggtaaa
    15541 gaagtcaccc taaattctac
//
LOCUS       DACADA010000108        15313 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.110, whole genome shotgun sequence.
ACCESSION   DACADA010000108 DACADA010000000
VERSION     DACADA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.110"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            43..609
                     /locus_tag="ISB17_003299"
     CDS             43..609
                     /locus_tag="ISB17_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP1981198.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            613..1188
                     /locus_tag="ISB17_003300"
     CDS             613..1188
                     /locus_tag="ISB17_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAP1981199.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1230..2282)
                     /locus_tag="ISB17_003301"
     CDS             complement(1230..2282)
                     /locus_tag="ISB17_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP1981200.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2507..3427
                     /locus_tag="ISB17_003302"
     CDS             2507..3427
                     /locus_tag="ISB17_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAP1981201.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3599..4825
                     /gene="mdtG"
                     /locus_tag="ISB17_003303"
     CDS             3599..4825
                     /gene="mdtG"
                     /locus_tag="ISB17_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HAP1981202.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            4908..5282
                     /locus_tag="ISB17_003304"
     CDS             4908..5282
                     /locus_tag="ISB17_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HAP1981203.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5283..5510)
                     /locus_tag="ISB17_003305"
     CDS             complement(5283..5510)
                     /locus_tag="ISB17_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP1981204.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5682..8225)
                     /gene="mdoH"
                     /locus_tag="ISB17_003306"
     CDS             complement(5682..8225)
                     /gene="mdoH"
                     /locus_tag="ISB17_003306"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAP1981205.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8218..9753)
                     /gene="mdoG"
                     /locus_tag="ISB17_003307"
     CDS             complement(8218..9753)
                     /gene="mdoG"
                     /locus_tag="ISB17_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAP1981206.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10147..11304
                     /gene="mdoC"
                     /locus_tag="ISB17_003308"
     CDS             10147..11304
                     /gene="mdoC"
                     /locus_tag="ISB17_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAP1981207.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNLRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11312..12793)
                     /locus_tag="ISB17_003309"
     CDS             complement(11312..12793)
                     /locus_tag="ISB17_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAP1981208.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTCLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(12735..13268)
                     /gene="ymdB"
                     /locus_tag="ISB17_003310"
     CDS             complement(12735..13268)
                     /gene="ymdB"
                     /locus_tag="ISB17_003310"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAP1981209.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13363..13674)
                     /locus_tag="ISB17_003311"
     CDS             complement(13363..13674)
                     /locus_tag="ISB17_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1981210.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13795..14127)
                     /gene="csgC"
                     /locus_tag="ISB17_003312"
     CDS             complement(13795..14127)
                     /gene="csgC"
                     /locus_tag="ISB17_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAP1981211.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14187..14645)
                     /gene="csgA"
                     /locus_tag="ISB17_003313"
     CDS             complement(14187..14645)
                     /gene="csgA"
                     /locus_tag="ISB17_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAP1981212.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14686..15141)
                     /gene="csgB"
                     /locus_tag="ISB17_003314"
     CDS             complement(14686..15141)
                     /gene="csgB"
                     /locus_tag="ISB17_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAP1981213.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3813 a         3927 c         3635 g         3938 t
ORIGIN      
        1 acgccatttt ctttctctcg caaattcgtt ttggatgtac ttatgtcatt cacaaatacc
       61 cctgaacgct atggtgttat atctgcggcc ttacattggc taagcgcaat tattgtctac
      121 ggcatgtttg ctttaggtct gtggatggtc acgctcagtt attacgatgg ctggtatcac
      181 aaagcacccg aactgcataa aagcatcggt attttgttaa tgatggggct ggttattcgc
      241 gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc cgagttattc gcccatgact
      301 cgtcttgcag caaaagctgg acatctcgca ttgtacctat tgctgttcgc catcggcatc
      361 agcggctatc tgatctcaac tgccgatggt aaaccgatca gcgtttttgg ctggtttgac
      421 gtccccgcga ccctctccga cgctggcgca caggctgact ttgccggtgc cctgcatttt
      481 tggctcgcgt ggagcgttgt agtactgtcc gttatgcacg gatttatggc cctgaagcat
      541 catttcatcg ataaagacga cactctgaag cgcatgctgg gaaagtcgtc atctgactat
      601 ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct tcgcttcctt gatgttctct
      661 gccggttcag cggttgccgc cgattacaaa attgacaaag aaggtcagca cgcctttgtt
      721 aacttccgaa tccagcatct tggctatagc tggttatatg gcaccttcaa agatttcgac
      781 ggtactttta cctttgatga aaaaaatcca gctgccgata aagtgaatgt gacaattaac
      841 accaccagcg tcgatactaa tcacgccgaa cgcgataaac atcttcgcag tgcggatttc
      901 ctcaataccg caaaatatcc acaggcaaca ttcacctcca ccagcgtgaa gaaagacggt
      961 gacgaactgg atattaccgg caatctgacg ctgaatggtg taaccaagcc cgtcacactg
     1021 gaagcgaaat taattggtca gggtgacgac ccgtggggcg gtaaacgtgc aggctttgag
     1081 gccgaaggca aaattaagct caaagacttc aatatcaaga cagatttagg tccagcgtct
     1141 caggaagtgg atctgattat ttcagtggaa ggcgtacagc agaagtaaga gtgaaatgtt
     1201 gccggatggc acgcatccgg caatgatatt tattctgttg gatcaggaat gcccagtgtt
     1261 gtattcagac gtccacgaga cttattaaag atcttattgc cattttcacg tcctgcccga
     1321 cggcgtcgct gttcctctgg cggtaacgcg ctttcttcgc agcaaatctc actacaacaa
     1381 cctttgtatt tttccgcaca tactggacac tgaataaaca gcagatggca gccatcattt
     1441 ttacagttgg tatggctgtc gcacggcgca ccgcactggt ggcagtgcgc gataatctca
     1501 tcagatatac gttcgcccat ccgctcgtca aaaacaaaat ttttgccaat aaaacgcacc
     1561 ggcaagccct gctcgcgcgc cttacgggcg tattcaataa ttccgccctc gatatgccag
     1621 actttgttga atccgttatg tttcatccag gcactggcct tttcacaacg aatgccgccg
     1681 gtgcagtaca tgacgatttt tttatcttta tgtgcctgca tcatctcaac tgcttttggc
     1741 agctgctcac ggaaggtatc tgccggtatt tccagcgcgt tttcaaagtg ccccacttca
     1801 tactcatagt ggttacgcat gtcgataaac aatgcatcgg gatcgtcaag catggcgttc
     1861 acttccgccg cttgcagata ctcaccaaca ttgctggcat caaagtgagg atcgtcaata
     1921 ccgtcggcaa caatgcgatc gcgtaccttc atacgcagta cccagaagga tttcccgtca
     1981 tcatccaacg cgatattcag gcgtaaaccc tctaaagccg gatcgaaggc atagagctgt
     2041 gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt gcgcgttaat gccctcatgc
     2101 gccagataca ctcgcccaaa aacattcagc gcggtaaaca gttgatataa agcgtcacgg
     2161 gtcaccttag gatcggcaat gtggaaatac ttataaaacg aaatggtggt tcgcggttcg
     2221 ctctcagcca acattttggc ttttaacgcg tcgttggaaa tgcggttgtg taacactggc
     2281 atggtgtacg gttcctgcga gatgggaaag taaaaatccg cggcatgata tagcaattat
     2341 cgataattaa catccacaca ttttacgcta cattttagca ttaaaaatta tttgttattt
     2401 acaagcgcgg caatttcgcc cagtcttcag tcacaaattt tggttgcgga cgaaaaaatg
     2461 cgacaataca tacaattgcc cgtataggtt gaaaaacagg attgatatga cgaatctacc
     2521 caagttctcc accgcactgc ttcatccgcg ttattggtta acctggttgg gtattggcgt
     2581 actttggttg gtcgtgcaat tgccctaccc ggttatctac cgcctcggtt gtggattagg
     2641 aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt gtgcatcgca acctggaact
     2701 gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg gtggtgaaga atttcgaatc
     2761 cgttggcatg ggcctgatgg aaaccggcat ggcgtggttc tggccggacc gccgaatcgc
     2821 ccgctggacg gaagtgatcg gcatggaaca cattcgtgac gtgcaggcgc aaaaacgcgg
     2881 catcctgtta gttggcatcc attttctgac actggagctg ggtgcgcggc agtttggtat
     2941 gcaggaaccg gggattggcg tttaccgccc gaacgataat ccactgattg actggctaca
     3001 aacctggggc cgtttgcgct caaataaatc gatgctcgac cgcaaagatt taaaaggcat
     3061 gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca ccggatcatg attacggccc
     3121 gcgctcaagc gttttcgtcc cgttgtttgc cgttgagcag gctgcgacca cgaccggaac
     3181 ctggatgctg gcacggatgt ccggcgcatg tctggtgccc ttcgttccac gccgtaagcc
     3241 agatggcaaa gggtatcagt tgattatact gccgccagag tgttctccgc cgctggatga
     3301 tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa aaatgcatca tgatggcacc
     3361 agagcagtat atgtggttac accgtcgctt taaaacacgc ccggaaggcg ttccttcacg
     3421 ctattaaatc ttccatgctg gatgcttcag aatggcatcc ggcattacca cagaaaatcc
     3481 ccctgattta gcgataaaag ctctctggat tgcgccccct ggaagtcggg cgcataatta
     3541 gtgtgcttat cttttcttct tatgttcacc gcgcctggcg caccaacagc ggattgctat
     3601 gtcaccctgt gaaaatgaca cccctataaa ctggaaacga aacctgatcg tcgcctggct
     3661 aggctgtttt cttaccggtg ccgccttcag cctggtaatg cccttcttac ccctctacgt
     3721 tgagcagctt ggcgttaccg gccactccgc cctgaatatg tggtccggta ttgtcttcag
     3781 cattacattt ttattttcgg ccatcgcctc accgttttgg ggtggactcg ccgatcgtaa
     3841 aggccgaaaa ctcatgctat tacgctctgc tctcggcatg ggcatcgtga tggtgttgat
     3901 gggactggca caaaatatct ggcagttttt gatcctgcgg gcgcttcttg gtttacttgg
     3961 cggatttgtc cccaacgcta atgctcttat cgccacacaa gtaccgcgta ataaaagcgg
     4021 ctgggcgctg ggtacgctct ccacaggcgg cgttagcggc gcgttgctcg gcccaatggc
     4081 tggcggtttg ctcgccgata gctacggctt acgtccggta ttctttatta ccgccagtgt
     4141 gctcatactc tgctttttcg tcaccctgtt ttgcatcaga gaaaaattcc agccggtcag
     4201 caaaaaagag atgctgcaca tgcgggaagt ggtgacatca cttaaaaacc cgaaactggt
     4261 actcagcctg tttgtcacca cgttaatcat ccaggtggcg acgggctcaa ttgcccccat
     4321 tctgacgctg tatgtccgcg aactggcggg taacgtcagt aacgtcgcct ttatcagtgg
     4381 catgatcgcc tcggtgccag gcgtggcggc tctgctaagt gcaccacgac tcggcaaact
     4441 tggcgatcga atcggacccg aaaagatcct gattacggcg ctgatctttt ctgtactgct
     4501 gttgatccca atgtcttacg ttcagacgcc attgcaactt gggattttac gttttttgct
     4561 cggtgccgcc gatggtgcac tactccccgc cgtacagaca ctattagttt ataactcgag
     4621 caaccagatc gccgggcgta tcttcagcta taaccaatcg tttcgtgata ttggcaacgt
     4681 taccggacca ttgatgggag cagcgatttc agcgaactac ggttttaggg cggtatttct
     4741 cgtcaccgct ggcgtagtgt tattcaacgc agtctattca tggaacagtc tacgtcgtcg
     4801 tcgaatcccc cagatatcga actgattttt cgcctttcat acttgcaaaa gcggagaatc
     4861 agctatcctt ttccctgaaa cctcatcaac tcaaagggag aatcgtgatg accatgtacg
     4921 caacgcttga agaagccatt gacgctgcac gcgaagaatt tcttgcagac aaccccggca
     4981 tcgacgccga aaacgcgaat gtgcaacagt tcaatgccca aaaatacgtt ttgcaggacg
     5041 gcgacatcat gtggcaagtt gagttttttg ccgacgaagg ggaagaaggt gaatgtttac
     5101 ccatgcttag cggtgaagct gcgcaaagtg tctttgacgg cgactatgat gagatagaga
     5161 tccgccagga gtggcaagat gagaatacat tacatgaatg ggacgagggg gaatttcagc
     5221 ttgagccacc gctggatacc gaggaaggac gcacagcagc tgatgagtgg gatgaacgtt
     5281 aatcactcat acgggccatg atgaatgtcg atcggcagca gtaaggtatc gaaaaccagg
     5341 gagaatggca gatcaaggat cgtgacgtaa cgccaggcgg agtcacgcac gtcccattgc
     5401 acaccgggat aatattggtt gccatgcccc tgccccggta tagtgcgact aatgatgctg
     5461 ccacagccac tcagcaaggt caccattatg ctcaccacaa ttaaacgcat ttgatacctc
     5521 ttccgtaaaa aataccggca taacgccggt atttttattg gctcgttgtt tttgccggat
     5581 gcggcgtgaa cgccttgcca gacccacaaa atcttgcaaa atcaataaat tgcaggaacg
     5641 atgtaggcct gataagcgta gcgcatcagg caactacgtt tttattgcga agccgcatcc
     5701 ggtttacgca atgccagtgg attgagcttt atcccttcgt aataactcac ccatgaagaa
     5761 tatctctccg gggaattcca gacgcggaaa tgcagacggg ccatcgtcac cggatcgctt
     5821 agcagcacca ggcgacgatc gcgattcagc ttctctggcg tctcgttcag cgcctgttca
     5881 acgtggcggt cacgggcgat ttccagcacc ttgctggcgc ggtgacgcgc ggtcgccatt
     5941 gcggttgcca gagcgttaaa tgacgggtta aacaccgcgt gcataaaacc atcatcaagg
     6001 gagcgttgac gattcatctc aaggaaccga tcggtatcaa ccagcacctg cggcggtgaa
     6061 tactcttccg ggatcaggaa cagtttccag cgtttggttc gcagaccaac ggtggcacgg
     6121 ctggaaatca ccgaaacaaa cggtgacagg atcaacgaga agacaatcgg tgccagccag
     6181 aacagaaaac gcagatccag ccacgccatc ccaacagccc acactaaccc taacagcagc
     6241 tgtgagccgt ggcgtttgaa cgcttccccc caggaagtgg agtcatcatc acgctgcggc
     6301 gaattccaca ctacttccca gccaaggaac gcgctgacga cgaagaccgt atggaacagc
     6361 atgcgtaccg gagccagcag cacggaaaac agcacttcca gcagcaacga taatgtaacg
     6421 cgccagaagc cgccgtattc tttcgttcct ttgcaccaga taagcaaaat gctcaatagc
     6481 ttcggcagga acaacagcac catggtcgaa gcaaaaagtg caatcgccag ctcaggacgc
     6541 cactgcggcc ataccgggaa caactgccgt ggttgcagga agtattgcgg ttcggtcaac
     6601 gcatgtacta cctgcaatgc agtagagagc gcgaggaaca taaaccacag cggagcggag
     6661 agataagaca tcacgcccgt caggaacacc gcacggtgaa ccgggtgcat acccttcacc
     6721 aggaacagac ggaagttcat caggttaccg tggcaccagc ggcggtcacg ttttagctca
     6781 tcaagcaagt taggcggcaa ttcttcataa gaacccggga gatcgtaagc aatccagacc
     6841 ccccaacctg cacggcgcat caacgccgct tccacgaagt catgtgacag gattgaaccg
     6901 gcaaaagaac cttcgcccgg cagcggagcc agtgcacagt gctcgataaa cggtttcacg
     6961 cggataatcg cgttatgccc ccagtagtgc gactcgccaa gttgccagaa gtgcaaaccg
     7021 gctgtaaaca gtggcccata cacgcgggtc gcgaactgct gacagcgcgc atacagcgta
     7081 tccatgccgg acgctttcgg cgacgactga atgatcccgg cgttcgggtt ggcttccatc
     7141 aggcgcacca ggccgcacaa acaatcaccg gtcattaccg agtcagcatc cagcaccacc
     7201 atgtagctgt actggctgcc ccagcgacgg cagaagtcat cgatattacc gcttttacgc
     7261 ttcacgcgac ggcggcggcg gcgatagaaa atctgacctt cgccaccgac ttcagcgata
     7321 agctccatcc aggctttttg ctctgcgacg cagatatccg ggttataact gtcactcaga
     7381 atgtagacat caaagtgttt ggcattcccg gtggctttta ctgattccca cgttgcacgc
     7441 aagccagcaa aaacacggtt cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga
     7501 tgctccgggt ttaatggttc atcgccaact gttgacgcag atatactgta tttatcgcga
     7561 ccaataagca gttgcaggaa gcccatcaac gccgtccaga acccggcgga cacccaacag
     7621 aacagtaccg caaagaggat caggataccg gtttgcagca tataaggcag aagctgcata
     7681 aaggaaaccc acacatcctg accaaccata tccataggat taatcagcgc ccacccctga
     7741 taaggaagaa tggtcttcat ataccaggtc gcgacaacag tttgcgcgag cgtcaggatc
     7801 aacagaatgt aacggcggat ggtaccgacg gtacgccact tttgctcact ctcctgctct
     7861 tctttggtca aacgagccag atagcgcggc gtcacatcgc gtccacgcag gcgatcccag
     7921 aaacggccta ccgggttggt acgccacggg tcgggaaaca tcgaggagcg ttttgcttct
     7981 ggcatcgcct ttagctgatc gcgcccttcg tcgtctttaa ttaactgtcc atcagcaagt
     8041 gaatctggcc aggcttgttc cagacgcgcc tttaccgagc cttgcgggga gtcatcctcc
     8101 cgcgcccagg tgcggtgttc ggcatccagc gcctgatgaa cggcgcggat atcagtcttc
     8161 ggcaatgccg ctttctcgct tgcggcgatg ggcattgcgt caatgtactc agttgtctta
     8221 ttcattggca ggtaactggt agctccaggt ttcactcaac gtctgatcgg cattcaccag
     8281 cgcagcacgc atttcagtgg ttttcttggc atctttcact ttcacacgca tcaccagacg
     8341 ccaacctttg gtaaccgggt tatagcgcac cgtgctttca actatctcac cattatcacc
     8401 aatgctggtt tgcgctgtga ccggggtatc ctctggcagt tttttcatct cagcgccggt
     8461 aaaatcgacc acaaaggcga tagtaccgtc aggctggcga atcaggttcg actgcttcac
     8521 atcccccgtt gaacgacgcg tttgttgcac ccatgcgtta tctggcgcat gcagtttgtc
     8581 ttcatcacgg ctgaaggtga tggtgtattt aaagttcatc tctttacccg gctccggcag
     8641 ctgatccggc gtccagtaag cgacgatgtt atcgttggtt tcatcgttag ttggaatttc
     8701 caccagctca acgctgcctt tgccccactc ccctttcgga gtcacccatg cgcttggacg
     8761 aagatcgtaa cgatcatcga gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag
     8821 accgaagcct tgcgggtttt ccatcgagaa gctgctgaca gctaaatgtt tcgggttatt
     8881 caacggacgc cagatccatt cgccattacc agcatggata gacagaccgt tagagtcgtg
     8941 caactccgga cgatagttat ttgcaggcga cggttggttc ggcccaaaca ggaacatact
     9001 ggttaacggt gcaaccccca gtttgccgac tttatcgcgc agatagattt tcgactgcac
     9061 atccacaacc gtgtcacgtc ctggcataac tacgaatttg taagcacctg tcgcgcgcgg
     9121 cgagtcaagc aatgcataaa tggttaaacg tttatcagtc ggttttggac gctcgatcca
     9181 gaactctttg aagcgtggaa attcttcacc cgatggcaag gcggtatcaa ttgccaggcc
     9241 gcgtgcagaa aggccataaa cctgacctgc accaatcacg cggaaatagc tggccccgag
     9301 catgctgacg atttcatcgt ttttatcttt gctgttgatc gggtaaagca ctttgaaacc
     9361 ggcaaaacca aggtctttta ccgtgtcttt gtcatgctga acatcgccga aagtgaaata
     9421 atccgggctg tatttgattc gtttgactgc ggtggcagtc acttcattta ttttgaccgg
     9481 ggtatcgaag tacataccct gatggtagaa ctcgagtttg aatggggtct tcagattgtt
     9541 ccagtacgct ttgtcatgat taaactggat ctgctgatag tccgcgtatt tcatatcgcg
     9601 gaaaacggag ggcaagttgc ttttgggcgc ctcatagcct ttcccggcta aggattgagc
     9661 ttgctttgcg acatcatcaa tactgaaagc ccagcttgaa gatgtataca gggttaacat
     9721 tactgcagca ctcaaccaac gcattttcat catttgtagt ttatgtttca taattagtaa
     9781 gcacttcccc ctttgtgtgc ttatatcgat ccgatctatt ttaatggata acctgccatt
     9841 ccgacaacag aatcccggta tcgttcagca cactggctca tggattaagc acctgattgc
     9901 aaccgttttc actttccgtg cttctcctaa agacagaagc atcgacttcg tgtagggttt
     9961 gccagcaaat ttatttatca accgtcttac agacgcttcc attctggtta gtggtagcga
    10021 tgatgctatg cataaaaacc ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcactat
    10081 ggcaacaagc ctaaaggcat aacccgacag ggataagacg aaaaatctga gatcagttaa
    10141 ccatatatga atccagtacc cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg
    10201 ctgatgttgt tagggatccc ttttcatatt tctttaatct attcgagcca tacatggcat
    10261 gtgaatagcg ccgagccgtc attgtggctg acccttttta atgacttcat ccactcgttc
    10321 cgcatgcagg tgtttttcgt tatatccggg tacttttcct acatgctttt tttacgctat
    10381 cccttgaaaa aatggtggaa agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc
    10441 atccccttac tgacattgcc gcaatttatt atgctgcaat acgtcaaagg caaagcggaa
    10501 agttggcctg ggctgtcatt gtatgacaaa tataatacgt tggcctggga attaatatca
    10561 cacctgtggt ttttactggt gttagtggtc atgacgacgc tgtgcgtatg gatatttaaa
    10621 cgcatcagaa ataatttaga aaattctgat aaaacgaata aaaaattctc gatggtaaaa
    10681 ttatcggtga tttttttgtg cctcggcatc ggttatgcgg taataagaag aacgattttt
    10741 attgtgtatc cacccattct gagtaacggc atgttcaatt ttattgtcat gcaaacgtta
    10801 ttttatttgc cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc
    10861 ttgtttacca cgccttctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat
    10921 ttactcaacc tgcgctatgg cagtggcgat gcctggatgt atgaaaccga gtcggtgatc
    10981 accatggtcc tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt
    11041 aacttccagt cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt
    11101 caccacccgt taacgctgtt tttcggcgca tacattacac cacacatcac ctccaactgg
    11161 cttggttttc tctgtggcct gatattcgta gtagggattg cgataattct gtatgaaatt
    11221 catttacgca tcccattatt gaagtttttg ttctctggta aaccggttgt taagcgtgag
    11281 aacgataaag cacctgcccg ttaagccgcg tttacaataa ccattccacg ggcaatatcg
    11341 acgccagttt gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa
    11401 tctctttctt tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct
    11461 gccaggccgc atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt
    11521 cgctctctat cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga
    11581 aagagccaat aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggcac
    11641 cggaattacc ggttataccg cgatcgtgta atgtactact ttgttcacgc gtcggcttga
    11701 gttcatataa ttccacccca tagcgaagca attttttgcg ccagcgcgcg tatccggcat
    11761 ggacgacagc aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt
    11821 ttctcaccat ccgtaagagt tgcgccacac ctgcacgtgt cggtacaaaa taggaagaga
    11881 taatatcgat gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg
    11941 aatgccgttt tgccttgccc tcccctttcg ccggatcatc acttaataaa catgttttcg
    12001 cccagataag cggcaacgtt ccatcaacca gatgatgtat aaacggactg gattccattt
    12061 tgcgtaagta acgatgcgtc atgacatcgt tatgccagga ggcgggtaac tcgatgtgat
    12121 ccgccatttc accttccggg acatccagca cctgctgtaa gggggaaacc gatttgcaat
    12181 accagtagcg ggcgaaatca tcggcaacgt cctctaccac gggtcctatt gccatgacat
    12241 ctaaatccga aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgtc
    12301 ctcccaccag cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga
    12361 gacgggaaaa gtcggtgata taaccaagcg gacgtagcaa acgaaacgag aaaggattaa
    12421 aaagccggac ttcaatgcgt ggatggctgt caagcaagcg taaaatgtca tcaagtccgg
    12481 gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca
    12541 gggcggaaaa cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca
    12601 gcgtatgctc tgccatttca gccagacgat accgggcggc aaacgcatcc agacttttct
    12661 ccagaggaaa aagtccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg
    12721 ccagccgagg caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg
    12781 ttttcttcat cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat
    12841 tctgaaacgg tttttactgc gatttcagcc gctgccgcac ggggataacc ataaacccca
    12901 gtactgattg caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgtaggcta
    12961 ttgagatagg catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc
    13021 ccgacggtgt gcactacggc tttagcggga agatcgcctg caagcgtaat aacggcatgc
    13081 cccgtagggc aatcgccctg ctgctgcctg acttttaaac aggcatccag cagggccgga
    13141 cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc
    13201 gcattcacaa tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc
    13261 gttttcataa cgcctcctta caccttcttg tttcctaagt gtacagcagg ctttttagtt
    13321 aaatcgcatt tttctgctta gcaacgtgag ttgttcagaa acttagttat attcgatgtt
    13381 cattacagcc agctttttct gtttatcaag atactgcact tttactgagg ctatttgcac
    13441 gtttttaaca ttgccgctca ttaaggcttt gctggaatag gttttacgcg gaacagaacc
    13501 attctgtggg cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc
    13561 cacaatttgg ccataaaaat ggatcacgcc cccttgcgcg gtgcttcctg caatggcaat
    13621 aaaaggaaaa aacaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc
    13681 tatttcctca atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg
    13741 attcgcagca gaccattctc tccaggttca tcttatgctc gatatttcaa caaattaaga
    13801 cttttctgaa gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt
    13861 aacaactatt ttcacccgat cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat
    13921 gggttgatta gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact
    13981 gccttcgcgc agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc
    14041 aggaataatg gtgtacatat ccccttgctg ggtcgtatta aaggttatct gactggaaag
    14101 tgccgcaagg agtaataacg cattcatatt cttctcccga aaaaaaaaca gggcttacgc
    14161 cctgtttctt tcatacagat gatgtattag tactgatgag cggtcgcgtt gttaccaaag
    14221 ccaacctgag tgacgttaac gctggagtta gatgcagtct ggtcaacagc agcaccgtta
    14281 ccgccaccga actgtttaac agtcatagta gaatctttac cattccactg atcaagagta
    14341 gcgctgttgc cgaaaccacg ctgagtcaga tcgattgagc tgtcatcaga accttggcca
    14401 acatctgcac cattaccgcc gccgtgctgg gtaatggtca gatcagagtt gcgggcgtca
    14461 gcttgcagag caagagcaga gttaccgcca ccgtactggt aaatattcag ctctgaattc
    14521 gggccgctgt tattaccgcc accaccgtgg ttgccgccac cgccgccgta ctgaggaaca
    14581 acacctgcca gagcgctacc ggagaatacg attgctgcaa ttgctgctac ttttaaaagt
    14641 ttcatgtaaa acccccatcg gattgattta aaagtcgaat ggaaattaac gttgtgtcac
    14701 gcgaatagcc atttgcgact gtctctgcac tacaattgcc gttttttgag taccatactg
    14761 tgtaatattt gctttattac cagaaccttt ctggataatc atcgcagtat taccataagc
    14821 accttgcgaa atactggcat cgttggcact gcccgcctga tcaatatatg caaggttata
    14881 atctcctgtc tggtcaatct ttgcccggtt gctactacct tcttgcgcaa caaccgccaa
    14941 aagttttgag cctccctgcc gtaactgagc actattatta gtcccagctt gaccaattat
    15001 ggctgcctga ttaaatgaag acttactcaa ttcatttacc gcaaagttat attctgaatt
    15061 agctaaatca taacctgctg cggctgcaat cccaggcgca cccagtattg ttaacatcat
    15121 aaataacaat ttgtttttca tgttgtcacc ctggacctgg tcgtacattt aagaaattaa
    15181 atcatttcaa cttggttgtt aacgcaaccc gtattttgtt aacgctgcgt tacgatggaa
    15241 agtatgtctg cggaaatatt tttaataact cacccgcgcg ttgtattttt ctttttttgt
    15301 tcataccaaa gta
//
LOCUS       DACADA010000109        15137 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.111, whole genome shotgun sequence.
ACCESSION   DACADA010000109 DACADA010000000
VERSION     DACADA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.111"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            14..553
                     /locus_tag="ISB17_003315"
     CDS             14..553
                     /locus_tag="ISB17_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1981214.1"
                     /translation="MYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSPGSLPF
                     TTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLG
                     WIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKL
                     AAKQHYKNAERLLAGLSAH"
     gene            630..848
                     /locus_tag="ISB17_003316"
     CDS             630..848
                     /locus_tag="ISB17_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1981215.1"
                     /translation="MMPRQPGPPSSTGIGCGCGSDNAGEGVDQDYKQAMYWYLKAAAQ
                     ESVGAYVNIGYMYKHGQGVEKDYQAAFE"
     gene            complement(1098..1580)
                     /locus_tag="ISB17_003317"
     CDS             complement(1098..1580)
                     /locus_tag="ISB17_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAP1981216.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1815..4397
                     /gene="leuS"
                     /locus_tag="ISB17_003318"
     CDS             1815..4397
                     /gene="leuS"
                     /locus_tag="ISB17_003318"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP1981217.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4412..4993
                     /gene="lptE"
                     /locus_tag="ISB17_003319"
     CDS             4412..4993
                     /gene="lptE"
                     /locus_tag="ISB17_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAP1981218.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4993..6024
                     /gene="holA"
                     /locus_tag="ISB17_003320"
     CDS             4993..6024
                     /gene="holA"
                     /locus_tag="ISB17_003320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP1981219.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6026..6667
                     /gene="nadD"
                     /locus_tag="ISB17_003321"
     CDS             6026..6667
                     /gene="nadD"
                     /locus_tag="ISB17_003321"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP1981220.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6691..7302
                     /locus_tag="ISB17_003322"
     CDS             6691..7302
                     /locus_tag="ISB17_003322"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAP1981221.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7562..7879
                     /gene="rsfS"
                     /locus_tag="ISB17_003323"
                     /gene_synonym="rsfA"
     CDS             7562..7879
                     /gene="rsfS"
                     /locus_tag="ISB17_003323"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP1981222.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7883..8350
                     /gene="rlmH"
                     /locus_tag="ISB17_003324"
     CDS             7883..8350
                     /gene="rlmH"
                     /locus_tag="ISB17_003324"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP1981223.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8381..10282
                     /gene="mrdA"
                     /locus_tag="ISB17_003325"
     CDS             8381..10282
                     /gene="mrdA"
                     /locus_tag="ISB17_003325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAP1981224.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10285..11397
                     /gene="mrdB"
                     /locus_tag="ISB17_003326"
                     /gene_synonym="rodA"
     CDS             10285..11397
                     /gene="mrdB"
                     /locus_tag="ISB17_003326"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAP1981225.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11408..12496
                     /gene="rlpA"
                     /locus_tag="ISB17_003327"
     CDS             11408..12496
                     /gene="rlpA"
                     /locus_tag="ISB17_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAP1981226.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12636..13847
                     /gene="dacA"
                     /locus_tag="ISB17_003328"
     CDS             12636..13847
                     /gene="dacA"
                     /locus_tag="ISB17_003328"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAP1981227.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13957..14220
                     /locus_tag="ISB17_003329"
     CDS             13957..14220
                     /locus_tag="ISB17_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAP1981228.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14321..14962
                     /gene="lipB"
                     /locus_tag="ISB17_003330"
     CDS             14321..14962
                     /gene="lipB"
                     /locus_tag="ISB17_003330"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAP1981229.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3751 a         3862 c         3927 g         3597 t
ORIGIN      
        1 gaataaaaaa ttaatgtata tattcgcaat atttatagtt gcagcaataa cctgtattag
       61 ccaacccaag aaaacgacgt tgcgtgataa agccatggcg aattatgcct ttgattattt
      121 aagctcaccg ggcagtcttc cattcaccac ggcagccacg gagctttccg cgattcatgg
      181 tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg acggtaaacc
      241 actggattat acacaggcga gatactggta tgagcaatca gcagaacagg aaaatccacg
      301 cgcgcaaagt aaactggggt ggatctacct caaaggtctg ggggtcaaac ccgacacccg
      361 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
      421 tttgggtttg atctacagaa atggctcagg tattaatgtt aaccattatg aatctcaaaa
      481 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccgggct
      541 ttccgcacat taacgtcgac tgtcacccga acagatggag tattttccct atcaaaccag
      601 caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc ccaccatcgt
      661 caacgggcat tggctgcggt tgcggctccg ataacgctgg cgaaggggtg gaccaggact
      721 ataagcaagc gatgtactgg tatctgaagg ctgctgctca ggaaagcgtc ggcgcttacg
      781 tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat caggccgcct
      841 ttgaatagtt tacgatagcc gctgaatgca atgacgccac agcctggtat aacctggcca
      901 ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc gacttgtatc
      961 gtaaagttca gtcatccgga accagagatg tcagtcaaga aattcgtgag attgaagatt
     1021 tactgtagag ataaaaacaa gggccgggga atttcattcg gcccatattc tctttattgc
     1081 tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc atgaccacat
     1141 ttagggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg
     1201 cagaccagat ttcccagccc taccacttct ccgctgtgat aaaccccatg atgattgagg
     1261 tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc cagctcctgc
     1321 cacaagcttt ctttaatcac ccgcataaag acgctgtcag actcttcttt caggctctct
     1381 tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag ctcatcgacc
     1441 tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg
     1501 tcgatatcac gttcgccatt gcgcaggcgt tcgctcagtg acgcgaccag ttcacggtaa
     1561 tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga
     1621 cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtatgaata
     1681 atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg gcgtttgggc
     1741 tatgctatgc ggatctgaaa aaccacatca acgctacatt tgtagccgta ttgaaaacag
     1801 gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc
     1861 ttcactggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt
     1921 actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac gttcgcaact
     1981 acaccatcgg tgacgtgatc gcccgctacc agcgcatgct gggcaaaaac gttctgcagc
     2041 caatcggctg ggacgcattt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg
     2101 cgccagcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg
     2161 gctttggtta tgactggagc cgcgagctgg cgacctgtac gccggaatac taccgttggg
     2221 aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataagaag acttctgcgg
     2281 tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct
     2341 gctggcgctg cgataccaaa gttgagcgta aagagatccc gcagtggttt atcaaaatca
     2401 cagcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg
     2461 ttaaaacaat gcagcgtaac tggatcggtc gttccgaagg cgtggaaatc accttcaacg
     2521 ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc tttatgggtt
     2581 gtacctacct ggcggtggct gcgggtcatc cgctggcaca gaaagcggcg gaaaataatc
     2641 ctgaactggc cgcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg
     2701 cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc ggttcaccca ttaacgggcg
     2761 aagaaattcc cgtttgggca gcaaacttcg tattgatgga atacggcacg ggcgcagtta
     2821 tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca
     2881 tcaaaccggt tatcctggca gctgacggct ctgagccaga tctttctcag caagccctga
     2941 ctgaaaaagg cgtgctgttc aactctggcg agtttaatgg tcttgaccat gaagcggcct
     3001 tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc
     3061 gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt ccgatggtga
     3121 cgctggaaga cggtaccgta atgccgaccc cggacgacca gctgccggtg atcctgccgg
     3181 aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa
     3241 ctaccgttaa cggtatgcca gcactgcgtg aaaccgacac tttcgacacc tttatggagt
     3301 cctcctggta ctatgcgcgc tacacttgcc cggagtacaa agaaggtatg ctggattcca
     3361 aagcggctaa ctattggctg ccggtggata tttacattgg tggtatcgaa cacgccatta
     3421 tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact
     3481 ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact
     3541 atgttggtga aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg
     3601 acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg
     3661 gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac
     3721 gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc
     3781 tcgaatggca ggaatccggc gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac
     3841 tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgtcgat gcgctgactg
     3901 aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagta accgatgata
     3961 tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac
     4021 tggcgaaagc accgaccgat ggcgagcagg atcgcgccct gatgcaggaa gcgctgctgg
     4081 cggtagtccg tatgcttaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac
     4141 tgaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg
     4201 tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgca aaaatcaccg
     4261 ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag
     4321 caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt aaactcctca
     4381 atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacat tgttgttatc
     4441 tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca cgcaggttcc
     4501 ttccactatg aaagtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc
     4561 ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa
     4621 ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt
     4681 ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat
     4741 ccccggccgt gacatctacc cgattagcgc caaggtcttc cgttcgttct tcgataaccc
     4801 gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg
     4861 tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcagata ttcgttccga
     4921 cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccac
     4981 gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg
     5041 ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac
     5101 gctgttcgtc aggtcgctgc ggcgcaaggg ttcgaagaac accacacttt ttccattgat
     5161 cccaacaccg actggaatgc gattttttcg ttatgccagg ccatgagcct gtttgccagt
     5221 cgacaaacgc tattgctgtt gttacccgaa aacggaccga atgcggcgat caatgaacaa
     5281 cttctcacac tcaccggact tctgcatgac gatctactgt tgatcgtccg cggtaataaa
     5341 ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag
     5401 gtgacctgtc agacaccgga gcaggctcag ctgccccgct gggttgctgc gcgcgcaaaa
     5461 cagctcaact tagaactgga tgacgctgca aatcaggtgc tctgctactg ttatgaaggt
     5521 aacctgctgg cgctggccca ggcactggag cgtttatcgc tactctggcc ggacggcaaa
     5581 ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat
     5641 tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct tcagcaactg
     5701 cgtctggaag gcagcgaacc ggttattttg ttgcgcacat tacaacgtga actgttgtta
     5761 ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat
     5821 cgggtatggc agaaccgccg gggcatgatg agcgaagcgt taaatcgctt aagccagccg
     5881 cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac
     5941 ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca taaacccctg
     6001 gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt
     6061 tgatccggtg cactatggtc atcttaaacc cgtggaaacg ctggcgaatt tgattggtct
     6121 gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa
     6181 cagcatgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct
     6241 tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg
     6301 gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg attcattgct
     6361 gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg
     6421 tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga
     6481 tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc
     6541 tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga
     6601 atcgtgtgag gatttattac cggaaccggt actgacttac attaaccaac aaggcttgta
     6661 tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggtgaa
     6721 acgcaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct gaccgcgcgc
     6781 ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg atgtttcctt tgacctggtt
     6841 ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttcttag tgaccgccag
     6901 caccccgttc atatcatccc tgaactcaat gaaatgtttt ttggcgactg ggagatgcga
     6961 catcatcgtg acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg
     7021 cagcaagcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaacga
     7081 tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttagtcgt cagccatcag
     7141 ggcgtactga gtctgttaat tgctcgttta attggtatgc ctgccgagtc tatgtggcat
     7201 ttccgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta
     7261 cgcgtcctca atagccgtgc catcggggtc gagaatgcat gacttttctg tttttttacg
     7321 ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc agccagactt
     7381 tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc
     7441 gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg
     7501 cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa
     7561 cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca
     7621 ggacatcatc gccttagacg ttcagggcaa atccagcatc accgactgca tgatcatctg
     7681 tacgggtacg tccagccgcc atgttatgtc cattgctgat cacgttgtgc aggagtctcg
     7741 cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt
     7801 ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact
     7861 ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc
     7921 ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt
     7981 cgagctgatt gaaattccgg ccggaaaacg cggcaagaat gcggacatca agcgcatact
     8041 cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga
     8101 tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct
     8161 ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa
     8221 agcggcggcg gagcagagct ggtcgctgtc ggcacttacc cttccccatc cgctggttcg
     8281 cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca
     8341 ccgtgagtga taagtgagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt
     8401 tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg
     8461 gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt
     8521 taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcacccag
     8581 ccgcggcatt atctacgacc gtaacggtat ccctctggct ctcaaccgca ctatctacca
     8641 gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg ctttgcgcag
     8701 cgtggttgat ctgaccgatg atgatattgc tgcattccga aaagagcgcg cacgttcaca
     8761 ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa gtacaagtag ctcgctttgc
     8821 cgtcaaccag taccgttttc cgggtgttga agttaaaggc tataaacgtc gttactatcc
     8881 ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt
     8941 cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg atatcggtaa
     9001 gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt
     9061 tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaggccgg
     9121 acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc
     9181 gggtagccgc gcagctgtgg tagtcaccga tccgcgtacc ggtggggtgt tggcgctggt
     9241 ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc
     9301 cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc
     9361 tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttgagcgccg gagtgatcac
     9421 gcgcaatacg acgttgtttg accccggctg gtggcaactt ccaggttcgg aaaaacgtta
     9481 tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc
     9541 tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg
     9601 gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg
     9661 caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt atcagggtga
     9721 caccattccg gttggtatcg gtcagggtta ctggacagcg accccaatcc agatgagtaa
     9781 ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc tgatgagcac
     9841 cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg tcggcgatat
     9901 tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa
     9961 cggtacggcg cataaatatt ttgccagcgc accgtacaaa attgcggcga aatccggtac
    10021 cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg
    10081 tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt
    10141 cgccatgatt ctggagaacg gtggcgcggg tccggcggtt ggtacgctga tgcgccagat
    10201 cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt
    10261 tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat
    10321 aaagtccatc tcgatcccac aatgctgctg atcttactgg cattgctggt ttacagcgcc
    10381 ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa aatcggccag
    10441 attgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa
    10501 ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc
    10561 ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg ttttcaaccg
    10621 tcggaaattg ccaagatagc cgtaccgttg atggttgcac gctttatcaa ccgcgacgtt
    10681 tgcccacctt cattgaagaa tacagctatc gcgctggtgc tgatatttat gcccacgctg
    10741 ctggtggctg cacagcctga cctgggaaca tcaatcctcg ttgcgctttc cggcctgttt
    10801 gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtagcg
    10861 gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg
    10921 atgctcctgg acccggaatc agacccactc ggcgcaggct atcacattat tcagtctaaa
    10981 attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac tcagtcacag
    11041 cttgaatttc tccccgaacg ccataccgac tttatcttcg cggtactggc ggaagagctg
    11101 ggattagtgg gcattttgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg
    11161 tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggcgg cttaatgctg
    11221 atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta
    11281 ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct gatggctggg
    11341 ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag
    11401 gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg
    11461 tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg
    11521 ccccatagtt gaaattagcg gggcagaccc gcgtttcgaa ccactgaacg cgacggcaaa
    11581 tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag
    11641 ccaggcggga ctggcggcaa tctatgatgc cgaacccggc agtaacctga cggcctctgg
    11701 cgaagctttc gatccgacac agttgacggc ggcccatcca acgcttccga tccccagcta
    11761 cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg
    11821 cccttatggc aacgaccgcg tgatttcgct ttctcgcgcg gcagctgacc gtcttaacac
    11881 gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc
    11941 tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc cagcacctcc
    12001 cgatttaagc ggtggcgcgg gaacaagttc agtgcctgtc ccgcagggtg acattcttcc
    12061 ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagcagcgg
    12121 tttcctcggc gcaccaacga ccttagcgcc tggtgtactg gaaggcagcg aaccgacgcc
    12181 tgcgccacag cccgttgtta cagctccgtc gacaacgcct gcaacctcac ctgcaatggt
    12241 gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt
    12301 tagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc
    12361 cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat tcgccagcaa
    12421 agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat
    12481 taccaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta
    12541 acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt
    12601 tttttaattc catcacggat gtcgtagttc agaccatgaa taccattttt tccgctcgta
    12661 tcatgaagcg cctggcgctc accacggctc tttgcacagc ctttatctct gctgcacatg
    12721 ccgatgacct gaatatcaaa accatgatcc cgggtgtacc gcagatcgac gcggagtcct
    12781 acatcctgat tgactataac tccggcaaag tactcgccga acagaacgct gatgtccgcc
    12841 gcgatcctgc cagcctgacc aaaatgatga ccagttacgt tatcggccag gcaatgaaag
    12901 ccggtaaatt taaagaaact gatttagtca ctatcggcaa cgacgcatgg gccaccggta
    12961 acccggtgtt taaaggttct tcgctgatgt tcctcaaacc gggcatgcag gttccggttt
    13021 ctcagctgat ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc gccatggccg
    13081 attttgccgc tggtagccag gacgcttttg ttggcttgat gaacagctac gttaacgccc
    13141 tgggcctgaa aaatacccac ttccagacgg tacacggtct ggatgctgat ggtcagtaca
    13201 gctccgcgcg agatatggcg ctgatcggcc aggcgttgat ccgtgacgta ccgaatgaat
    13261 actcgatcta taaagaaaaa gaatttacgt ttaacggtat tcgccagctg aaccgtaacg
    13321 gcctgttatg ggataacagc ctgaatgtcg acggcatcaa aaccggacac actgacaaag
    13381 caggttacaa ccttgttgct tctgcgactg aaggccagat gcgcttgatc tctgcggtga
    13441 tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa aaaattgcta acctggggct
    13501 tccgtttctt cgaaaccgtt aatcctctga aagtaggtaa agagttcgcc tctgaaccgg
    13561 tttggtttgg tgattctgat cgcgcttcgt taggggttga taaagacgtg tacctgacca
    13621 ttccgcgtgg tcgcatgaaa gatctgaaag ctagctatgt gctgaacagc agtgaattgc
    13681 atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa cttccagctt gacggcaaaa
    13741 cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc ggaaggtaac ttcttcggca
    13801 aaatcattga ttacattaaa ttaatgttcc atcactggtt tggttaaaaa ttgaacactt
    13861 gaaagtgtaa tttccgtccc catatactaa gcatcagtaa aaaactcccg ccttctggcg
    13921 ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact taacgaactg
    13981 cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc tgagctggtt
    14041 gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc aacggtaaaa
    14101 ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac tcatatcgag
    14161 caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat ggttctgtaa
    14221 ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc tgtgatatac
    14281 ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg ataaaattct
    14341 tgtccgccag ctcggtcttc agccttacga gccaatctcc caggctatgc atgaattcac
    14401 cgatacccgc gatgaaagta cccttgatga aatctggctg gtcgagcact atccggtatt
    14461 cacccaaggg caggcaggaa aggcggagca cattttaatg ccgggtgata ttccggtgat
    14521 ccagagcgat cgcggtgggc aggtgactta tcacgggccg gggcaacagg tgatgtatgt
    14581 gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct tgcttgagca
    14641 aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg ctgacgcgcc
    14701 tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgtattc gacgcggttg
    14761 ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt tacgtattaa
    14821 tccttgtggg tatgcaggaa tggaaatggc taaaatatca caatggaaac ccgaagcgac
    14881 gactaataat attgcaccac gtttactgga aaatatttta gcgctactaa acaatcctga
    14941 cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac tgggtcatta
    15001 ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc tttatcactt
    15061 gcagaaatgc gatgctacgc cttgctccgt agcccaccta attgattaac tcattgaaat
    15121 aattgaccat ggtgagc
//
LOCUS       DACADA010000110        15077 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.112, whole genome shotgun sequence.
ACCESSION   DACADA010000110 DACADA010000000
VERSION     DACADA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.112"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(223..966)
                     /gene="traF"
                     /locus_tag="ISB17_003331"
     CDS             complement(223..966)
                     /gene="traF"
                     /locus_tag="ISB17_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAP1981230.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKISPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEYYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPKF"
     gene            complement(959..1216)
                     /gene="trbE"
                     /locus_tag="ISB17_003332"
     CDS             complement(959..1216)
                     /gene="trbE"
                     /locus_tag="ISB17_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAP1981231.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKADERKNDERNEDE"
     gene            complement(1243..3093)
                     /gene="traN"
                     /locus_tag="ISB17_003333"
     CDS             complement(1243..3093)
                     /gene="traN"
                     /locus_tag="ISB17_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAP1981232.1"
                     /translation="MKRILPLILALVTGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSVHAGLYFL
                     NSRYDFMNTTFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3090..3728)
                     /gene="trbC"
                     /locus_tag="ISB17_003334"
     CDS             complement(3090..3728)
                     /gene="trbC"
                     /locus_tag="ISB17_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAP1981233.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQETLSQQL
                     REKPDIQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3737..4042)
                     /locus_tag="ISB17_003335"
     CDS             complement(3737..4042)
                     /locus_tag="ISB17_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981234.1"
                     /translation="MATMDSKYIQHLRKIGAVLLRLISRTLNLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCVFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(4072..5064)
                     /gene="traU"
                     /locus_tag="ISB17_003336"
     CDS             complement(4072..5064)
                     /gene="traU"
                     /locus_tag="ISB17_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAP1981235.1"
                     /translation="MKRRLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5061..5693)
                     /gene="traW"
                     /locus_tag="ISB17_003337"
     CDS             complement(5061..5693)
                     /gene="traW"
                     /locus_tag="ISB17_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAP1981236.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRIYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5690..6076)
                     /gene="trbI"
                     /locus_tag="ISB17_003338"
     CDS             complement(5690..6076)
                     /gene="trbI"
                     /locus_tag="ISB17_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAP1981237.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(6073..8700)
                     /gene="traC"
                     /locus_tag="ISB17_003339"
     CDS             complement(6073..8700)
                     /gene="traC"
                     /locus_tag="ISB17_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAP1981238.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLKS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNYKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAARDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8860..9081)
                     /gene="traR"
                     /locus_tag="ISB17_003340"
     CDS             complement(8860..9081)
                     /gene="traR"
                     /locus_tag="ISB17_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAP1981239.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(9216..9731)
                     /gene="traV"
                     /locus_tag="ISB17_003341"
     CDS             complement(9216..9731)
                     /gene="traV"
                     /locus_tag="ISB17_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAP1981240.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKELEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATPPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9731..10090)
                     /locus_tag="ISB17_003342"
     CDS             complement(9731..10090)
                     /locus_tag="ISB17_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAP1981241.1"
                     /translation="MFISLMTGQTETTEIKMNMRNIITARSVPDKSVSDDFMNAVLSN
                     CTTRIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNL
                     TVELISAVVESNKKGEG"
     gene            complement(10029..10616)
                     /gene="traP"
                     /locus_tag="ISB17_003343"
     CDS             complement(10029..10616)
                     /gene="traP"
                     /locus_tag="ISB17_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAP1981242.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTLGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAAD
                     AADYVRETDRSLMQLVTAFWATLALMYVSLAAITGKYPVRPGKMKCVRVGTADEHLKE
                     VYTEDTSLSEKIRKCHVYFPDDRANRNNGDKNEHA"
     gene            complement(10585..12036)
                     /locus_tag="ISB17_003344"
     CDS             complement(10585..12036)
                     /locus_tag="ISB17_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HAP1981243.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVMYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPQGGDAGKEGGNGG"
     gene            complement(12036..12740)
                     /gene="traK"
                     /locus_tag="ISB17_003345"
     CDS             complement(12036..12740)
                     /gene="traK"
                     /locus_tag="ISB17_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAP1981244.1"
                     /translation="MFGSLFFSCSVMAANGTLSPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGACMDVYVIRDGEGN"
     gene            complement(12751..13317)
                     /gene="traE"
                     /locus_tag="ISB17_003346"
     CDS             complement(12751..13317)
                     /gene="traE"
                     /locus_tag="ISB17_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAP1981245.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLKYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13339..13650)
                     /gene="traL"
                     /locus_tag="ISB17_003347"
     CDS             complement(13339..13650)
                     /gene="traL"
                     /locus_tag="ISB17_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAP1981246.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWV
                     K"
     gene            complement(13655..14017)
                     /gene="traA"
                     /locus_tag="ISB17_003348"
     CDS             complement(13655..14017)
                     /gene="traA"
                     /locus_tag="ISB17_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAP1981247.1"
                     /translation="MDAFLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            complement(14051..14278)
                     /gene="traY"
                     /locus_tag="ISB17_003349"
     CDS             complement(14051..14278)
                     /gene="traY"
                     /locus_tag="ISB17_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAP1981248.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(14366..15043)
                     /locus_tag="ISB17_003350"
     CDS             complement(14366..15043)
                     /locus_tag="ISB17_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP1981249.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
BASE COUNT         3549 a         4187 c         3549 g         3792 t
ORIGIN      
        1 aatctgctcc atgcgggaga ggaaaatctc ttttcgggtc tgacggcgct tagtgctgaa
       61 ttcactatcg gcgaaggtga gttgatggct catgatgtcc ctctgggatg cgctccggat
      121 gaatatgatg atctcatatc aggaacttgt tcgcaccttc cttaacagaa caacacctgt
      181 aaacattttc aaataatctt cactcataaa tcaaatccgc gattaaaatt ttggtttaaa
      241 atcttcagaa acgttcagga attgttttgc caggtcgtcc tgcgaaataa agccgtatga
      301 taacgggcga acactgccct gtttcgggtc aaccagcatc atggccggga aatatttcac
      361 gccgaggcgc tgcgcctgcc cctggtcagt ccgggaatcc ggcaacagcg gattaatcac
      421 gccatccacg gaaacgggaa taacactcag accatacgta tcccggaagc cattaatgac
      481 ctgcgccagc tgcccgtcga tggggtcctg cccccggtaa aaaaacatga tgccgtagta
      541 ttcagccagt ttcgcaatgg cctgtcgctg ctgcgcctgg tctgccgcca gctggttccg
      601 taccgtgccg ttgtaatggc tgtactgcag gttatagtcc agttcgggat gtgccagcat
      661 ggccttttta gcactcatgg tgaaaagccc ggcctgctga gtccagtaat tctgcagccg
      721 gaaatatttc acaaaattat ccacgccggg atacagaatg gcttcgtaca gcgcccgctt
      781 tgttgccgtc tgcagcgcgg ccagtttctg cataatatcc ggctcctgac ggggggctgc
      841 aggtacaggt ttattttctt tttcctttgg acttattttc tcgttatacc actgccagcc
      901 tgcatctttc cctgacgccg gaaaaataaa gcagcagagt aacagtggca gtaatgcttt
      961 attcatcttc attcctttca tcattttttc tttcatcagc tttctgcagg ccagtcaggg
     1021 cagaaaaaca aaaatacaga ataaacagga gcacaccgga gtaaaggata acgaaagcag
     1081 cggcaagcat cccgtctgcc gtggtttctt ttaccgtatc ccacgaccag ataattaccg
     1141 gagaaatgac aatcgctgta atcaaaagcc ggataagaaa atcaacaaga cgattagaag
     1201 tgaatatgac tttcatcatt tcccccggtt attttccatt tcttactgtc ctgcctgttt
     1261 cagctggtca gcaatctgct ctttcacttt ctgcgtcaga acgccactgt ccgggatttt
     1321 ctggttattc atcaggtctt cgtagaagtt agtgaaatcc agtctgtcga actgaatttt
     1381 ctgcagctca tcaaccgtaa tcccccgaca gtcaggatgc ttcgcactgc caaacccgat
     1441 acgcaactgc ccgttacgcc cctgctgctg gactatctgc gccagcttcg aatcaaactg
     1501 gcagtagctg cgctttttct gcaggcagac ccccagcacc tttttcgaac agaactcacc
     1561 cacactcacc gtcagtttat tgtcctttgc ctttcccagc gccttttctt cactgttgca
     1621 cttcgccagc cccacatcct gaccccagcc gccatcctta cagcagttac tgaagccggc
     1681 tgcggcctta cggcagaatt tcgcctcacc cgtgaaagcc ctgacatcca caccattcag
     1741 ggcggcaaca tccttacctg cggcggcaag cgcagccagc tgtgatactg cttcaccaaa
     1801 atcactgctt tttccgctct gtgccttatc gcattcaccg tcaaggcaga agacatcacc
     1861 accacagacc atcacttttc ctgatgtttt tgactcgcag gagtacgtgg cgtactcatg
     1921 cagacaggtt ccctcatcgg aataaaacgc gcactgacgt gaggccagcg tacaggccgg
     1981 attatcgaca tatttctgac aggtgccgtt atctgcggac tgagtgacat atttatcccg
     2041 gtatgcccag catgcctgtg tgatgctcca gggtttacct tccatcacac ccgttttcgt
     2101 accacccggc tctgaacact cagtgccttt cagcacgcca tccgctttat taaacggaca
     2161 actctccacc cactccaccc ggggaaccca ttctttatca cggaccttca tcctgagttt
     2221 cagcgtaaag gtggaagcac cactgacaag cgattcatag actttcctgt caccattccc
     2281 gtgcgggagg caattaccgt ttgcagtaca gccactaccg gtcagaacct gcccctgtgt
     2341 gacagaaaac ccggagggca caggtatggt gaaagtcccg ctctgtcctg taaccagttg
     2401 cacattaaag gttgtgttca taaagtcgta acgggagtta aggaaatata gccctgcatg
     2461 aaccgatagc gaggcacttt ccactgtccc ggcaacaggt acggtaacgc tgaacaccag
     2521 tttcagaccg ttcatactga accggaactg tgagtgcggt atcgtcacct cacgatattc
     2581 atccgtgtac ttccagtctc cggtgatact ggcagtccgg gagcagtatt cctccaccat
     2641 cgtgtcccgc tcacaggtat agtttgtgaa ctcactgcgg tttatctgct gcgcactgca
     2701 ctgctgcccg gtgtttccca caatgctgtc agcccggttc accacatccc tgccggtctg
     2761 gataaacggc gcatccggtg aaagaatgtc tttcggcttg ttcatgaatg actctgtgat
     2821 agtttttccg gtttcaccgg tcgcccattc cgtggtgccg tcatttttca ggccgccatc
     2881 cccgccggca gtcacgccac cgtaatattt tgtctcgtcc ggattcgcgt tatagccggg
     2941 gatactctcc tgtggcttaa agccctgaat actgctgctt ccctgccctt tgatctggtg
     3001 agcaaaatca gaaccggccc ggtaatcact gttgctgtca gcctgtgcca taccggtaac
     3061 cagagccagt atcagaggta aaatacgttt catttcccgg aatctccttt ccctgccagt
     3121 aaatcatgag ccacctgccg gcagtcaccc gtggcggcca ctttctccag cgcctggccg
     3181 acccgcagat ttccccggat gatgtcgtat ccctgactgc agaacaccac cagtgccggt
     3241 acgctgcgaa tgccgtactg tgaaaacagc gtcgggtcga tctgaacacc atcagttgcg
     3301 ccgtctttca ccagggacaa cacggcttca gcggtggtct tcaggtcatt gttcaccatg
     3361 ccccgcagtg tggccggaat accgtagtgc cgggtttcgc ccagcatccg tttcagcccc
     3421 tcttcgggaa tggagaagga cacaaagtac acggcgccct gctgtggttt atccttctga
     3481 gcagcctgct gtttacggac cagttcatcg agaaactggt tatcggaatg ctgaagggga
     3541 ttcgccctga cctgctgctc tgcccatgcc tgcaactgta tgtccggctt ttcacgtaac
     3601 tgctgactca gtgtttcctg ctgcttcagg aactggcggt tttcagttgt gttcacattt
     3661 tctgaagcat gaacagcaag atgcatccct gctgtcagag caaaaagtgc aaatattttc
     3721 cggttcatga tatttctcac tttctgctat ttacaggcat gaactcatca gaaaaaagat
     3781 aattgagaaa aacagccata aacacacatg taaatgtcac cttcagatat aatctgacaa
     3841 gtgtcacttg ttctgatata tctgccttct tccattcatg aataatttca gcgagaaaac
     3901 tgtcaggtgc aaaataaaat aatataacag gcaccattac aagaccaaaa ggaaataaat
     3961 ttaatgttct gcttatcagc ctgagcagca cagcacctat tttcctgagg tgctgaatat
     4021 atttactgtc cattgttgcc attttcattt cctcttattc attaaaatga tttacaggaa
     4081 tacacagtta cgtttacgcc acatcaggta gccgaagttt ttctttgtgt tgggcggatt
     4141 ttttcccgtc tcccagcgca tcacactgcg cccgaacgga tggcactgtc cgctgtccgg
     4201 atacatattc accatctgat aacgccagcg ttctttgggc aggattgggg acggatattc
     4261 attacagacg gcgttatttt tcccgatggt ttccataatc atgccctgac ggtgcagctt
     4321 gaatgccatg cgttcactga ccagcaggga ggactgcaac ggactggact cattactcac
     4381 ccagccattg aacgggtaca tactgccctg cgaaccggca caccagaaca gaacatcgag
     4441 aggcatatta aaggcactgg caatggcatc tgctgcgcag gccccctgtg ctatcggatt
     4501 ggcaaaaatg acggcttccg ggttgagaat ggtggtcagg ctgctgtccg tccaggtggg
     4561 gtcgatttca gaaagataag cgatatccag gtcaccacct tccagacagc ccagcgatgt
     4621 gatgatgttc agccagtacg tcagcgggta tttgtaccag tgaacgtgat agaacgcccc
     4681 gttcacctgc ttctcatcct ttttcgccgt tccctgtgcc gttttaccaa aagccggcag
     4741 gctgaagccc aggttcacca tgcatcccgg tgaccgggtg acgtccgtca gcgccatcgg
     4801 ctcccagtaa ccaatggcca gcccgatacg cctgaacagc ggcggcggtg ccggacaaat
     4861 ctgaataggc atcgacgggt tcgccgtgtc ggggacctta ccctggctga ctttgatact
     4921 gcccagcgag agcgggaaaa tacagctcca gcagatatcc gtgatcgggt ttacaaaacg
     4981 cccctcacag gcagaatccg cagacgccgc agggacatga ccggcgagaa ggaataacat
     5041 cagcagccac agtcttcgct tcattttctg ccctcctctg ccggaataaa ttccaccttc
     5101 aggaagcgat cgccgggaac ggcgctcacc cgtgccggga cctgatcaat gccgaggcgc
     5161 tggcagagca caccgttctg gtcaaagtaa atacggctgt ccagcgcttt ctgcatctcc
     5221 gggatactgc cctgcacgag gataattttg ctctccagtg tgggcggcgt ctggcgtttc
     5281 atccaggcca cctgcgccgg atcatcgcca ttgatgaaat acagcgtctg gttaaaagga
     5341 acatactgca gcggattcat cacttcaccc tgacgggcaa acacccgccc ttcgttatcc
     5401 cggatatccg cagccagtct gacggacggg tcaaacaacc ggctgctgta tttctccgtg
     5461 cgtccgatac cgggcacggc agggggccgc agactgttac ggatcacccg ctccttaaag
     5521 gcatccattt tccggcccat ctcaccggac tgctccagtg ccgtcaggcg ctgcatgatc
     5581 accgtcagca tgtccggctc ctttaccggc cacagatcgc cccaggtgcc aagatcggcg
     5641 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg
     5701 ctctcattcg ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta
     5761 ccacggcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
     5821 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt
     5881 tctgactggc gctgtcaaaa aatgcatcca cagtctgttt catgttgaag gcggcagtca
     5941 ccggtgcgtt cagtctgaca atgccgtaac tgacggctgc gttcagtaac accattgcca
     6001 ggcatcccgg caccgtccac caccagtggc tgcgtcgggg ggctgtaaca tcagtcgttt
     6061 tttgcgttgt ggtcatgcaa cactcctgta tttctcatgt tcttccagcc aggcttccag
     6121 cgaagccatc tccggccctg acttcttcca cgccagctgc cacaccgcct catgaatact
     6181 cagcccctct ttacgtttct gctgcacaaa ctcaaaatcc gggccgtcag agctgtacat
     6241 ggcgcgactt aacgggtcca caaacaggcg gtgccaggag gaatggtttt ccacctgcag
     6301 caggaaggaa ctgaaccact ggtctctggc cgcaccaaac ttaccaatca tgtcgcgctg
     6361 cagtggctgg aactggtccg gatacagctg gttgtatttt gcgaactcct tcgcactctg
     6421 tttgaggata attttgtagg aggagttacc ccatgccgca cgggccgcac tggaggcctt
     6481 gtcagagtca aagtcgacga tgttctgtgt gatggtgata taagcaccgg tatgacgacg
     6541 ggcggtacgg taacctttct caataaactc accgaccttg taatttttga agtccagcag
     6601 acgccagcct tcatcaatga cgttcagttt cttgaggtta cgcggcgtgc ggtacatcct
     6661 gttctcgatg tagataatca gggagaacat caccgcaacc agcagtgacg gacggtcttc
     6721 cagtccgccc agctccagca ccaccatttt tgcgtcatcc cgcagggacg gctcatcaga
     6781 gttaaaatac tgaccgtaag tgccgttggc agtgtactgg tcaagcagca caatcatttc
     6841 gtccagacgg ctgcggatag tcggtgaccc ggcatactgc tcgctgtcac tggcgttttt
     6901 caggaaatcc accacgtcat caatacgtgc cctgttctct ttggccagcc aggaggccct
     6961 gaccgcctgc agcagcagac cttcatgcac ttcatccagg ttaccgttgg ggctggccat
     7021 cactgacaac tggtcacgga cacgctccgc tgactggtcg atatcggtga tattcgcaaa
     7081 cgggttaaaa cgcagggttt caccgtccag atatacgcca cccatgttct cacacaggga
     7141 cttgtagcca tcccccatgt cgaacaccac ggcaaagccc ccggagtcca gcacgctgcg
     7201 gataaggggc tgtatcagtc cggttttacc ggccccggag gtgccacaga ccgccatgtt
     7261 gtagttggtg ttattcatcc cccggaagaa aatatcgata aacgccagct ggttacggta
     7321 ggtgggtgcc agcagacctg ccggtgtcag ggggttatcc gccactaacg gcatcaggtt
     7381 ggcaacatta aagctctctg cgcgctgaac cacaccggcc tctttcagct gtttaaacag
     7441 ccctttcccg gccataaagg gcaggcaggt caggaaattg cgcatgtggt taaagcgcgg
     7501 tgaaatcagc tcaaaaccgt ttttacggaa gctgttcagg atgtcctgct ccacttccag
     7561 tgccgtttca ttattgtcct tgcagaaggc tgtgatgttg aggaagtagg acacgacaga
     7621 agactgaccg gagcccagcc gctgacgcag ttccccccac tccttcgcct ctttctccac
     7681 ggacggaaac catttggcat aggaggtctt cgactttttc tccaggtcca tgtacttcag
     7741 gttggcttcg ctgtgggttt tcacctggtc ttccaccacc agagtcagcg tcaggatgaa
     7801 cggacaggag atggacagtt ccgggttcag caggttgctg tagttgtcgg ccatattcca
     7861 caggaaggcg atttccgggt tacgggccag atggaaattc aggatgcggg ccgtgctgtt
     7921 cctgccgttc tcacgcaggc ccagcgtcag gtaatcagcc cggactttca ggtcaaaact
     7981 gtcctccaca cactgataat tcagatcaga atacgggtcc agctgacgtc ttttcgggta
     8041 cagggagtcc gggttatgat taatcatctc cccgacaata tcgataaagg cctgtgcatc
     8101 caccgtctgt gtggcgatac tggccccctg taacgacgcc cggatgattt tcaccaggtt
     8161 ttccatttcc agaatgtcag cccggctttt tttcttcgag ggagaacagt acgagaagaa
     8221 tacccggtaa tggcgcaggg tcaggggcag attcatcccc tccggcagcg gaaactgtgt
     8281 cgctgccgcg ttcatataat aggcacgggt aatggcgtta aaccgttcag cctgttcacc
     8341 ggaccaggag aactcacgca gcccgtattc aattctgtca ccaacaagct ggctggactt
     8401 cagatggatg cagaacggca ctccgcgtgg cagtttggtg cgcagcatgt ggtcgagcgc
     8461 ctccacaatg gactcattcg ccccattgat gggaatggct tccagcataa agcccatcgt
     8521 ggtgtcattc atgaacagac cggactcctg gttgtaatca cggtacggca gcagacggct
     8581 gaactgcggg aagctcattt cgcccagaac ttcattggcc tttgcggatt cgtcaggcag
     8641 tttcagtgct gtgacgaggg agttaaccgc ctgagtgacg gcctcaagtg ggttattcac
     8701 atgaattcct tagttgaaaa ataaagcgag aaatgcggta gccatgaagg gaatacctgt
     8761 aattaacgcg ccatttcccg gcgcatatac aaagatttcg gttttctgaa tatcaactca
     8821 tgttcatccg gaattaaatc aatatgttct gcgactgact tatgcataat gttttctctg
     8881 cttttcctga tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg
     8941 ggcttccgga ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt
     9001 tattttctgg cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc
     9061 ttcatcggct tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc
     9121 atcccttttc acgcacaggg cactcgtttt acaggcagat tttgaataca gaaagcccgc
     9181 cggcattgcc ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac
     9241 gcagacggtt taataacaaa aaacacactg gatggctgat gataaacatc ctgattatca
     9301 atataaggcg ctatccataa tgcagccgtc tgctcacccg ttcttagcgg gcggggaggt
     9361 gttaccggcg caactgaact gaccggaaca atcgttttca cttcccgggc agccgtaaac
     9421 aacggacgtg gtgccagcag tttctgctcc ggaagtgccg tcaccgcagg tctgctgccg
     9481 gacggagggg tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg
     9541 cgcggcagtg atgccgcaac cggctttgct tctgaggact gctccagttc tttggccttc
     9601 tcattggcct gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc
     9661 gtactggttc cggcacagcc ggaaagtaat aacgttccca gcagaggaat aaataaagaa
     9721 atctgtttca ttacccttct ccttttttat tactctccac aacagcagaa attaactcta
     9781 ctgtcagatt tgtttttcct gcacagggtt gtccggagac tactgtattt cccggagaag
     9841 aaaataagct catcgacata cccttcttca tcacaccgac agctgccata ttaaaattgt
     9901 gcggcaatga ttcagaaccg aattccttca gtgcaggaag tacgattctt gtggtgcagt
     9961 tactgagaac agcattcata aaatcatctg atacgctttt atccggtaca gagcgtgcag
    10021 taataatatt acgcatgttc atttttatct ccgttgtttc tgtttgccct gtcatcaggg
    10081 aaataaacat ggcatttcct gattttttcc gacagagaag tatcttccgt ataaacttcc
    10141 ttcagatgtt catctgcggt tcccacccgg acacatttca tttttccggg tcgcaccgga
    10201 tatttcccgg tgatcgctgc cagcgagaca tacatcagtg ccagtgttgc ccagaatgcg
    10261 gtgaccagtt gcatcagtga ccggtcagtc tccctgacat aatctgcagc atcagccgcg
    10321 cggtaatggc agatatcctg tatttctgat ctgcctgaag aatcagagga agaaaaccag
    10381 cattcactga ccgggaactg tccggcaggt gctgtttgtc ttacgaagtt aatctcctta
    10441 accagtaatt tccccagcgt ggtgttgtcc ttccataaaa caaacagagc catcagggcc
    10501 attgtggcca gcggccagat aaccacaaac ctgacgaccc acagcagcca gcgaacgaca
    10561 aacgccacac ggaatgtcag tgcatcaccc gccatttccg ccctccttgc cggcatcccc
    10621 gccctgcggg ttcttgtctc cggacataaa ctgctgcgga ttcagattgc ccagctgctt
    10681 cagcatctgg tcggtgttaa aaccgttcag gtggctttct gccgagggag gattcggcac
    10741 agggctttcc gggttaccct cttccgctgt actctgtgac tgctccagcg ccatttcctc
    10801 cacggttttc agctggaagc cgtcctggaa caccactgtc acttcgttgc ccgcaccaat
    10861 cggtatcacc gggtgatact gttcggcacg tttgatgtag tagtcactga gcgtctgtgc
    10921 ggctttcgat gcgccgccac cgatacccat tttaaggaca tcgccggccc cgtaagcggc
    10981 tgtggcaccc agcccgacag ccggctggga ggcgcgctcc attccctgac cgataccgtc
    11041 aacaaatccc gcgccccatg cccagccgag gattttgccg ttacgcatca ccacttcgcc
    11101 cttgataccg tttttacccc ggaagctgac atgcccctta atcggcatat caatggtttt
    11161 gccattcttc aggcagctga tattacgggt gcgaacaata gcacgttcac tggagacatc
    11221 cccccaggct tccagcccca caaaacatcc cgttgcgtca tacgtcttgc tgttcggcat
    11281 ttccaccaga ccggtgatac gcagctgcat cggcaccgtg gattcattac cggtgactga
    11341 ggcattggca tccgcccctt caatcagcat ggctttcgca aaacttcctg acggaatgta
    11401 cggcagcgat ggtccctgtt ttccctcatt acgggtaaac accttacgct gtatccggtt
    11461 aggcaccggc acagactggt acatcacctg tggtggtggc gtgacaccat tcccgggata
    11521 aaacgccgtc ggcggtggaa cagcaacgct gccctgcggc gggaaggata cgggggtgtt
    11581 tcccggctgt ggttcgcctt ccggcccggg cggtgaggct ggcgtctgcg gtacaggctc
    11641 accggtcgcc gtgacgggat tagcacccag ggcttttacc tgctctgcca gggcggcatt
    11701 gtcctgtccc agtttttcaa tacgacgctg gtcatcaccg cgctgtttgt tcaggacatc
    11761 cagttcacga cggatttcct catactgctt ctgcatctgc gctgccgtca cctgcatctc
    11821 tgtggtggca tgctgacgca ccttgtcatc aaaggtcgta tccaccacac cggtcatatc
    11881 cggcacaggc tcctgttcag ccacggtttc cccgttaccg gacatgtcca catcagacag
    11941 atacagtgcc ccaccaatcg cggaggctgt accgacaacc acaatcccca gccacaggta
    12001 ctgcttgcgt ttcacaatgg tgttgatact ggccatcagt tgccctcccc gtcacggatg
    12061 acatacacat ccatgcacgc acctgccagt aactggctgg caggctggct gaacatcacg
    12121 gcacgggttc ccggctgcca gaagtcactt tcccggatat tcagggcaga cagcgtttta
    12181 ttttccacgg caaagcggac catcttcagg tgattccccg tccatacggc atctgccact
    12241 gaagacagcc ccgccggggc ctgcagggtt tcctttgtca caggaacctg ataccagcct
    12301 gcaggtaatt ttccgccacg gacagcctgg ctgatggtca caagcaggga ttcgtaaggc
    12361 gtggacgttt cccacgcacc ggcttcttct ccggttccgc gcaggtcact gaccagctga
    12421 atggtacgcc ccgcgccttc acggggaacg gcctgaatgg aaagattcag accacgttct
    12481 gtttccagaa tgaacgtaaa gggctttttg ttgacggtgg caaccaccac accgccggag
    12541 gcggtctgct cattattggt cagggcacca tccagactgt tcacggcgat aatccggtca
    12601 ccgggaacgg taaacagatt cgggctggta ttgctgatgg caatactggc ctgaccaccg
    12661 ttcaccattg gcaccacggt gggggacagc gtaccgtttg cggccatcac gctgcaggaa
    12721 aaaaacaggc tgccgaatat tattgccgta ttattttttc tcatcgtctg tttccccgaa
    12781 attatccagc caggtcaccc cgttttcccg cttcagaata aggatgtaat gtttgatatc
    12841 agtgaaaggt tttgaatcac caatccaggt tttaagcaca ccacgaattt ccacacggcc
    12901 atactgaggc cagacacgaa cactggtctg gaaaaaggct gagttcacgt tatcgttttt
    12961 aatacgcttc gcttcttcag ccagaataac tttcatctgg ttctgtgcgc ccgggcggat
    13021 atatttcaga agcgcctgat gtgaggcatc gacggtttct gatgaaacat tcagacggag
    13081 ggcaataaat gacagcgcca tctgctgtaa ataagaggcg tcggcactgt tctgtgacac
    13141 ggcaaagggg gcattaaatg ccattggcgt cacggcagtg cgttgctcat tctgaagacg
    13201 gtagttattc accccctgaa tgacgttaac agagaggctg agaacaataa gcactgacat
    13261 aaatataaag gcgatggcca ttacacgact ggtacttaaa cgggcaccgt gttccatata
    13321 acaatcctgg tatcagttct atttaaccca ctgccggaaa cacgaatcgg gaacattatg
    13381 aaaaataccg cgcagcaggg ctgttggcat ataccagtaa atcaggtctc gtaaccagga
    13441 actgccccgc ccttttttca gttttttaat cccgaaataa accagaaccg ctgcaccaat
    13501 accgaacaga tattttgatg ttgtgatacc ccagccaata cagattgcgg cggggatcag
    13561 ttcatccagt ggcaggccaa accagcggct ctggttggtc agtgtttccg ggaaacgata
    13621 tttcttaagt ttattctcgt ctcccgacat cgttttattt caggccaacg acggccatac
    13681 ccacagcaat aaatacagag atgatggcaa aaccggccag gaacttgacg tttttggtca
    13741 tcatgtacat gaccgcaccg accagaactt cagccagaac aacccattta acaacactgg
    13801 agtccttacc gaaggtcgcc ttaaccgtgg tgttaccgct tgccatcagg tcctggccct
    13861 gtgctgccat tgccagttgc gggaagaaca tcatcagaac agcagcaggg atcacagcgc
    13921 gagccaggcg aagcatattc agacgagtga atttagaaaa aaacgatttc tttttgacgg
    13981 gcgcagaagc accctgaaca cttaaaaagg cgtccatatg attcctctgt ttaatattga
    14041 tagataaaaa ctaaagttct ttaaatgttg actcgttctc ttcgatcacc tccctgaata
    14101 tttcttcatt atagaaatca ggaaaccgtt tcagatgatc tctgagacgg atctgaacct
    14161 ctatagtttt acttctaccg ctacgatctt tggcccgaat aagtcggtta tttgtatctt
    14221 catcgagata cactgagact gttctgctta tcccgcctct cgcgttacgc ctcctcactc
    14281 aattcacctc tgtttcctga ccaataaaat catgattgca cagaaatggc ctgtcgaaaa
    14341 tgggcccaaa acgagccctg ctttattaca tttttttaac acccatatat tcactcaaaa
    14401 catttaatat ttcgtacatt acacctgtct taatacattt attcctgaac aaatcgaagg
    14461 aacccaatct tttattgata tccgcattaa tctttttaat ccttttttta gaagccactt
    14521 ctgtgatttt taattcttta gctatattta tatgtttata ccctaatgca tagaaacaaa
    14581 aaacaaccaa tgattttgct gtcatattcg atataacaga ctgtacatag ctatcacgat
    14641 gataataatt ttctttttcc acacgaaaga atggatagtc aggaaatata ttaatttgcc
    14701 atagaacaac agattcttca ttataaaatg aaacattttc catccttatt tgatagattt
    14761 ctccatgaaa attaaaacga cgacaaaatg cggaaccatg cccaagagac tggcattcaa
    14821 gctcaagtga tgaaagaaaa atttcaacct gcagacgtac ataactttca ccagagaagg
    14881 gtttatcttc tttggaaaag agttcaataa aggccccatt ggcataaagg attttccttt
    14941 ttttgttacg gacacaaaca ggaacaggag aattcctgta gatattttga acgttaagaa
    15001 tgtatttatt tacagattga ctgttaagtg gcctttctct catcggcgat ttttaatatc
    15061 aagttttaac gtggcat
//
LOCUS       DACADA010000111        15052 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.113, whole genome shotgun sequence.
ACCESSION   DACADA010000111 DACADA010000000
VERSION     DACADA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.113"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(42..863)
                     /gene="dapB"
                     /locus_tag="ISB17_003351"
     CDS             complement(42..863)
                     /gene="dapB"
                     /locus_tag="ISB17_003351"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP1981250.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1024..1389)
                     /locus_tag="ISB17_003352"
     CDS             complement(1024..1389)
                     /locus_tag="ISB17_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1981251.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1412..2326)
                     /gene="rihC"
                     /locus_tag="ISB17_003353"
     CDS             complement(1412..2326)
                     /gene="rihC"
                     /locus_tag="ISB17_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAP1981252.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2392..3342)
                     /gene="ispH"
                     /locus_tag="ISB17_003354"
                     /gene_synonym="lytB"
     CDS             complement(2392..3342)
                     /gene="ispH"
                     /locus_tag="ISB17_003354"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAP1981253.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3344..3793)
                     /gene="fkpB"
                     /locus_tag="ISB17_003355"
     CDS             complement(3344..3793)
                     /gene="fkpB"
                     /locus_tag="ISB17_003355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1981254.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3901..4395)
                     /gene="lspA"
                     /locus_tag="ISB17_003356"
     CDS             complement(3901..4395)
                     /gene="lspA"
                     /locus_tag="ISB17_003356"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAP1981255.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4395..7211)
                     /gene="ileS"
                     /locus_tag="ISB17_003357"
     CDS             complement(4395..7211)
                     /gene="ileS"
                     /locus_tag="ISB17_003357"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP1981256.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7254..8195)
                     /gene="ribF"
                     /locus_tag="ISB17_003358"
     CDS             complement(7254..8195)
                     /gene="ribF"
                     /locus_tag="ISB17_003358"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAP1981257.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8203..8421)
                     /locus_tag="ISB17_003359"
     CDS             complement(8203..8421)
                     /locus_tag="ISB17_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAP1981258.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8524..8787
                     /gene="rpsT"
                     /locus_tag="ISB17_003360"
     CDS             8524..8787
                     /gene="rpsT"
                     /locus_tag="ISB17_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP1981259.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            9176..10549
                     /locus_tag="ISB17_003361"
     CDS             9176..10549
                     /locus_tag="ISB17_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981260.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
     gene            10577..12616
                     /locus_tag="ISB17_003362"
     CDS             10577..12616
                     /locus_tag="ISB17_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HAP1981261.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(12674..13573)
                     /gene="nhaR"
                     /locus_tag="ISB17_003363"
     CDS             complement(12674..13573)
                     /gene="nhaR"
                     /locus_tag="ISB17_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAP1981262.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(13639..14805)
                     /gene="nhaA"
                     /locus_tag="ISB17_003364"
     CDS             complement(13639..14805)
                     /gene="nhaA"
                     /locus_tag="ISB17_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAP1981263.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         3824 a         4005 c         3609 g         3614 t
ORIGIN      
        1 attaaatgtg ttatttttgc atcattataa atattttgtg gttacagatt attgagatca
       61 agcacatctc gcatatcaaa aagaccactt tccttaccac tcaaccacaa agccgatctt
      121 accgcaccgt tagcaaatgt catgcggctg gatgccttat gggtgatctc cagacgctcg
      181 ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg cacggtggca
      241 aaaccaatgg tgccaggcac acgttcaccg gtatggcctt cacgactgta gaccgcgcaa
      301 tctttcagat ctttatcaag agcgtgggcg atcgcctctc ccattgccag tgcggtgcct
      361 gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc ggtgtagtcc
      421 cccatcactt tggctgcttt ctccagcagt ttaagcatga cgttaacgcc aacgctaaaa
      481 ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaatcgcctg tttaccggct
      541 tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctggcgaca aaaagcgaga
      601 tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc atcttttatc
      661 gcatcaagac tactttgcac agtaacgcct gttttcccgg ctccggccag ttcgcccgca
      721 tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg cacgccttct
      781 aatgccagcg ccgcttgaat caactggcgg cccatacggc ccccggctcc cgcgatggca
      841 acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata gaccgttttc
      901 agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag aaattaatga
      961 taaacaggga gggatatcag tgaaaggcat gacgcagaaa gtgatgcgtc ataccctctg
     1021 gcattatgcc tggtcttgtg actttgacgg caggcagcac agtataagca gaattacgcc
     1081 cgtaacaggg atcaacacga caatggtaac ccaccaccct gaatatccga tatcgtgcat
     1141 ccttctgacg gctaacgcta tcgccggtac ggcaaaaatc aacgttaaaa taaatgacgc
     1201 gggcagcgca aaaaaaatga aaacaccata gtcattgtta attgttaccg ctaaccacca
     1261 acataaaagc ggaagcagac agataacgcc actaccaatc ataaacgacc agaactcgaa
     1321 gcgattggaa acaccttcat aattaaagat gtttttccac ccttccagat acgcttctcc
     1381 atacgtcata gaacacttcc ttgttttatt attagagcgc cagggccagc acctcagcca
     1441 cccactgctg gaagcctttc acatccagat ccagcgccac ctgtacattg gctggtttgc
     1501 ccaggcaacc gtcgatatca acaaccgtcg tgcctgaggt aaattcaccc tgagtttcta
     1561 ccgcgacaaa acacggtttg agtgtgaaca attccgggcg caccagccag gcgatagcgc
     1621 aaagatcgtg catacgtagt ccactttgca tactgccgct acggtagtgg ctaaacaggg
     1681 cgtgtagcat ttttccggtg cgattcaact ccggcaaagt agcgagatag tcaggggtta
     1741 atattgcctg attggtgaca tccagaccgc acatgacgat ttcgatgccg ctacggaaga
     1801 cacaggctgc cgcttctgga tcggcagcga tattaaactc ggcgtttggt gtgcagttgc
     1861 cgcgaccagc agaaccgccc atgatcacca gacggcgaat atacggctta cattccgggc
     1921 attgtgaaag taacagcgca atattggtta acggaccgat agccaccagg gtaacgggct
     1981 ctggcgcacg catcagggca tcacgaattg ccagaaacgc cggtataccc agtggctgac
     2041 ggttgtgttc aacaaagtta tagccagcca ttcccgattc accgtgtaca gatgccgcat
     2101 cgcgcggcgc acgaaccagt ggtacggcag cgccctgggc aagcggaata tccacattcc
     2161 agaaatgcag cagttgcagg gcgttgcggg tggttttctc taccgaaaca ttacccgcga
     2221 ctgtggtcat cagttgcaga tcgagttcgg gggtaaaaat cgcggcggca atggcaacgg
     2281 catcgtcaat gccggggtct gtatcgagga agataggtaa atgcatgttt tctccataaa
     2341 aaatgccggt aacaagaccg gcattttcgc ataacttaga ctactaatga cttaatcgac
     2401 ttcacgaata tcgacacgca gctctttcgg cacttcgaag acgatatttt cttcgcggcc
     2461 ttccagcgga atggcttcgc caccgccaag ctgctgcaag cgtgccacca cattctgcac
     2521 cagaatatcc ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca
     2581 ctcttcctgg atatctttcg catcgtcaat caaaaatgcg cgtttgccca tacgctgggc
     2641 cagttccgcc agacggttgg agttggagga gtttttcgaa ccaaccacca gtactacttc
     2701 cgcctgctct gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc
     2761 atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga
     2821 cgtgtcatcc accgacagcg tggtttgggt cataaaggag agcttctctt catttttgac
     2881 cgtcagtttc cacacatcgt ctggcgattc gaccagatac attccccctt ccgggttgct
     2941 gtactggccc atcgtccctt ccacttccgg gtgcccggcg tgaccgatga gaatagattc
     3001 ttcaccacga cggctggcgc gggcgacttc catatgcact ttggtcacca gcggacaggt
     3061 ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta cgtactgcct gagaaacacc
     3121 gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg ctaatctgct caataaagat
     3181 agccccacgc tcgcgcaggc tatcgaccac gtagcggtta tgcaccactt cgtgacggac
     3241 atatatcggt gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac
     3301 cccggcacaa aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg
     3361 gatcgatttc cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga
     3421 aatcaacggt aatggagtcg ccgttaattt cgcggatcac gccaggcatc tcactgccat
     3481 ccattgcggt aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac
     3541 ggcgggagaa gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca
     3601 gcgagaaggt ggttttatcg cccactttca gccccagcag gtgttgctcc agcccttcag
     3661 aaagagaagc atcacccagg cggaacagcg ccggtttacc gttgttgcgg gtagactcag
     3721 cggtggtgcc atcatcgagt ttcagcgtga agtgcaccag gacggcgcta ttgctctgta
     3781 cagattcaga catgcaggtt tgctcttata ttttgaggat ttttgccgga tgcggcctac
     3841 gatttcgcag caatctgtag gccggataag atgcgtcagc atcgcatccg gcagggttta
     3901 ttattgtttt ttcgctttag aaggtaaaaa accttccagc acaatcagtg ccgcaccgac
     3961 acaaatggca gtatcggcaa ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc
     4021 gatcatatcg acaacgaagc cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc
     4081 aataatcagc gcgtaagcga tattgtttag cttctgcgcg gccttcgagc gatacatcat
     4141 cactaccagg atcacgctaa taccaatcgc aataccggca aagaaccaac gctgccagcc
     4201 gccgctatcg gcaaggaaac taaacgccgc gccatagtta cgcgcataat gcagattaag
     4261 cgacgggaac agcgggaccg tatcccccag agcaaagttc tggaggatca ggtatttgct
     4321 gcccagatcg ataatcagca cgactaccac cagccacagc cagcgtagcc ctgttgaaca
     4381 gatcgattga ctcatcaggc aaacttacgt ttttcaccgt caccggcgac gttgctgaca
     4441 cagcggccgc agatttctgc gtgttccgcc accttgccga catcctgggt gtagtgccag
     4501 cagcgagggc acttctcacc ttcggcttta ctcaacgcga ctttcagccc tttgaggact
     4561 tcgctctgct gggcatcagc aggtgcgtcg ttatagtctg caacggtagc gccggaggtc
     4621 aacaggacaa atcgtaattc atcgcccagc gcggtcagtt tcgccgccag ttccggttct
     4681 gcatacaagg ttactgcagc ttccagcgag ccgcccactt tcttatcggc acgcgcttgc
     4741 tcaatgactt tgttcacttc gccacgcact ttcaacagct cgtcccagaa cgcatcgttc
     4801 attgcttcac tgtctgccag accaaacagg ccttcgtacc actcgccggt gaagacgtat
     4861 ttttcacgtt cgcccggcag gtagccccac acttcatcag cggtgaagga gaggattggt
     4921 gccatccagc gaaccagcgc ttctgcgatg tgatacagcg cagtctggca gctacgacgc
     4981 gccacgctgt ccgctttggc ggtatactga cggtctttga tgatgtcgag gtagaaggaa
     5041 cccatctcaa cggagcagaa gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct
     5101 tcgtacgcct tgaggatgtc ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc
     5161 agtaccacca tctcttccgg tttcaccata tcttttgccg gatcaaaacc gttcaggttt
     5221 gccagcagga agcgcgcggt gttacggata cgacgatagc tgtcggcagc acgtttcaga
     5281 atctcgtcag aaacagccat ttcgccggtg tagtcggttg atgccaccca cagacgcaga
     5341 atatccgcac ccagtttgtt catcacatcc tgcggcgaaa cggtgttacc gatggattta
     5401 gacatcttgc gaccctgacc atccacggta aagccgtgag tcagtacctg acgatatggc
     5461 gctttgccct tcatcgcggt agagatcatc agggaagaca tgaaccagcc acggtgttgg
     5521 tcagaacctt ccagatacat gtccgctgcg tgaccggcaa attccggacg cacatcaaca
     5581 acggaagagt gggtagatcc ggagtcaaac catacatcca gcgtatccgg tactttcaca
     5641 tactggtcag cttcgtcgcc gaggatctct ttcgcatcga gatcccacca cgcctggatg
     5701 ccgtcaactt caacgcgttt tgccacttct tccatcagtt cgagagtacg cggatgcagt
     5761 tcttctgtgt ctttgtgcac gaacagtgac atcggcacgc cccaggtacg ctgacgagag
     5821 atacaccagt caggacggtt agcaaccatc gactcgatac gcgcctggcc ccagtcaggg
     5881 atccactgca cgcctttgat ctctttcagt gactgcgcac gcagaccttt ctgatccatg
     5941 ctgacgaacc actgcggcgt cgcgcggaag atgatcggcg ttttatgacg ccagcagcac
     6001 ggatagctgt gttgcatttt ctcaacgtgc aacagtgcgc ctttttcctg caacaacgca
     6061 atgacaatat cgttcgcttt gaagacgtta acgccatcca gagtcgggta agtacccggc
     6121 agataagtgc cgtccgggcc aaccgggtta gcggtttcca gaccatattt ttgaccgatc
     6181 acatagtcgt ccggaccgtg gcctggcgcg gtatgaacgg caccggtacc ggcatccagc
     6241 gtaacgtggt cgccaagaat tgccggaaca tcgaagtcca taaacggatg ggtaaagcgc
     6301 aacagttcca gctcagcacc tttcaccgtg ccaagaatgg tgtaatcgct aacgccgata
     6361 cgctgcatta cgctttcaac cagatctttc gccagaatca cggcctgacc gtcgatttgc
     6421 accagcgcat aatcaaaatc aggtgcaatg gagattgcgc ggttagcagg cagcgtccac
     6481 ggcgtggtgg tccatataac cagcgaaatt gggccgttaa cattgcttac gccaaatttc
     6541 gttttcagcg catcctgatc gaccgcctgg aaagcgacgt cgatggacgg agaagttttg
     6601 tcgtaatact caacttccgc ttctgccagt gcagaacggc agtcaacgca ccagtgcacc
     6661 ggcttcgcgc ctttgtgcag gtgaccgttg ccgatgattt tgcccagcgc gcggatgatg
     6721 ttggcttcag ttttgaagtc catggtcagg tacgggtgcg accagtcgcc cagcacgccc
     6781 agacggataa agtctttgcg ctgaccgtca acctgggtcg cagcgtattc gcggcacttg
     6841 gcgcggaact cagcggcggt gaatttctcc cccggcttac cgtattcttg ctctactttc
     6901 agttcgattg gcagaccatg acagtcccag ccaggcacat acggcgagtc atatccagaa
     6961 agccctttgg acttaatgat aatgtctttc agaatcttgt taaccgagtg accaatatga
     7021 atgctgccat tcgcataagg agggccatca tgcagaatga aggttttttt gcctttttta
     7081 gctgcacgaa tgatgccgta cagatcatca tcagtccaac gcgccagcat tcccggttcg
     7141 cgcttggcga gatcgccacg catcgggaac cctgtttccg gcaaattcag ggttgattta
     7201 tagtcactca tcagattctc ggttccgtat ttcggtttga ttacataaca ggcttaagcc
     7261 ggttttgtta gcccaaaaaa ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc
     7321 agttcgtcca gcgatgcaaa tcgctgctca ttgcgtattt ttttacgcag cactacttgt
     7381 atatggcgac cataaaggtc cattgcaaca tctaacaaat gcacttccag ttgctggcga
     7441 ataccggcaa ccgttgggcg tgttccgatg tttgcaacgc cgggtaacgg cttttcgcca
     7501 aggcccaaca cttctaccgc ataaacccct ttcaccgggg aaacctgacg gcgtagcggt
     7561 acattcgccg tcgggaaacc tatagtgcgc cctaattcat caccgtggac tacacgcccg
     7621 gagatggcaa acgggtgccc cagtaaactt tctgccagag ccagattgtc atccgccagc
     7681 gcctgacgca cggcggtgct gctgatacgc acgccacctt cgcaaaaagt ttgcgtgctg
     7741 gtgatatcga agccgtattc catacccgct ttctgtaata acaagaaatc gccttcacga
     7801 ccagcgccaa agcggaaatc gtcacctacg gcaagaaatt ttacccgcaa gtgcttcacc
     7861 agaagatcac tgatgaagtt ttgcgcggtt aacgccgcaa aacgcctgtc aaaacgcacg
     7921 cacagcacgt aatcaacgcc acactctgca agataacgca gtttttcccg cagccgggtc
     7981 agccgtgccg gggctttatc agtagcaaac agttccagtg gttgaggttc aaaaagcatc
     8041 accatcaccg gtaagttgcg cttgcgccct tcttcctgca agccctgtaa cagcgcgcga
     8101 tgaccgcgat gcacgccgtc gaaattacca atagtcagca cacacccttc ttgcggggcc
     8161 tggctgagat tatgtatgcc gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg
     8221 tccgagtata ccttgtacag cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa
     8281 gaagtggctt ttcattgcta tggcgcaaat catgggaaga aactgaccgc ctactgcaat
     8341 ttttctcgcg gaaaagctgt attcacaccc cgcaagctgg tagaatcctg cgccatcact
     8401 acgtaacgag tgccggcaca ttaacggcgc ttatttgcac aaatccattg acaaaagaag
     8461 gctaaaaggg catattcctc ggcctttgaa ttgtccatat agaacacatt tgggagttgg
     8521 accttggcta atatcaaatc agctaagaag cgcgccattc agtctgaaaa ggctcgtaag
     8581 cacaacgcaa gccgtcgctc tatgatgcgt actttcatca agaaagtata cgcagctatc
     8641 gaagctggcg acaaagctgc tgcacagaaa gcatttaacg aaatgcaacc aatcgtggac
     8701 cgtcaggctg ctaaaggtct gatccacaaa aacaaagctg cacgtcataa ggctaacctg
     8761 actgcacaga tcaacaaact ggcttaattg cctgttgttg tcagctttgt aaaaaaaccc
     8821 gctctggcgg gtttttttat gcctgcggaa gttaccgtta atcgattata tattttctcc
     8881 atgaatacat cataaatata attgatgctt tttcattgtg aaagataaaa tatttcattt
     8941 ggctgtaaaa cgtgaactgt ataacaaata gcaggaaaac tgaataaaaa actaattaac
     9001 gcaaccttaa aacgaaaaat tataattgtc gctcttcccc actttaatta ctctcattgc
     9061 cacaacgttg ctttacatca ttaataaata cgcatcaagg atagttagca tgtatttgct
     9121 tagaaagtaa aagtcgtttt tctgagccag gcattcttgt tctggagaga atataatgag
     9181 ttccgtactt gaaactgata aatcatccgc atccgattca ctgcctttaa tacaacggat
     9241 cagctatggt tcgctggatg ttgcaggtaa tctgctctac tgttttggct ccacctacat
     9301 tctctatttc tacactgacg tggcgggcat aagccttgcc gtagctggca tcatcctgct
     9361 actggcgcgt attgtagacg gtatcgatgc cccggtatgg gggattatta ttgataaaac
     9421 gcattcccgc tacggtaaat gtcggccgtg gtttttatgg ttgccattac cttttgctgt
     9481 atttagcgca ctctcattct ggtcgccaga tatcagtatg acaggtaaaa ccatatattc
     9541 agcaatatcg tacatgttag ccagcattct ttttaccggt ttaaacacgc cgctaagcgc
     9601 aatattacct ttgatgacgt tatcaccaaa agaacggttg gtattaaact cttaccggat
     9661 gaccggcggg caaattggcg tgttattaat gaatgcgacg gcgctaccgt tagtggcttt
     9721 tcttggaaac ggtaatgata aggcaggatt tatttatact gctatcgtct tttccgtaat
     9781 ctcctgtgcg ctaacgctat ttgccttcaa aaatattcga gaactggata ccgacaaaat
     9841 acagcaggaa ccacgtctgc cgatgaaaaa gagcttttcg gcaatgaaag gcaactggcc
     9901 gtggatcctg atggtgctgg caaatctcat cttctggatt gccttacagc agcgcaacac
     9961 aaccattgtt tactatttaa cctacaacct ggatcgcaaa gatttagttc ccttgatcaa
    10021 cagcctcgcc accattcaga ttttgtttat catcgcaatt ccgtttttca gccgttacct
    10081 ctccaaaacc tggatttgga tcaccggcct gctggtcgcc atgttcggcg gaggactgat
    10141 gtggctggct ggagacaata ttcctttgat gattgccgcc tggatgctgg cgaatattgg
    10201 cagtggcatt gcctgctcta tgcccttcgc catgcttggt ttcgcggttg attttggtcg
    10261 ctggaaaaca gggattaaag ctaccggcat tctaatcgcc ttcggcagta ccttctgcat
    10321 caagatgggc agtggtatcg gcaccgcctt cgccgcctgg attatgaaca gctttggcta
    10381 tattcccaac cagccacaaa ctgccgccgg tctggacggt attagctggg cgtttatctg
    10441 ggtgccagcc gtgctctttg cactcgccgc cattccactg ctcttctttc gtaaatatga
    10501 agcgatggaa gagtgcatcc aacacgattt gcaagttcat aacccttaaa catgtcacaa
    10561 gctcctggag gaaattatgt cgtttattaa gcaagatccc catcgtctgg tctggcaaca
    10621 agacgatcgt tatctctgga ttgaagcatg gggtgagaac agtttacgtg tgcgtagcgg
    10681 tcgccatctg ccgatgatgc gcaatgaaaa ctgggcatta acagaagaaa aaggtgatgc
    10741 tgtcgcctat atcacatatg aagaaaagca ggcaacacta caaaacggca aaatcaccgc
    10801 cattgttaat ctgcagggtc agatctcttt ctggcgtaat gcagaaaaat gcctgctgca
    10861 ggagttctgg cgtcagcgcg gcgaaattgg cgaagatgag tcggcccacg gtcagtatgt
    10921 cagcgccctg aatttacagg cccgcgagtt caaaccgatt cccggcggga aatacacaat
    10981 caaggcgcgg ttcgaagcca acgatggcga aaagttgttt ggcatggggc agtaccagca
    11041 gccaaatctg gatttaaaag gctgtatgct agagctggcg cagcgtaatt ctcaggcatc
    11101 ggttcccttc ctgctttcta atcgcggcta tggttttctg tggaataacc cagcggtggg
    11161 tcgcgtcact ttcgcgcaaa atggcacgga atgggtggca gaagttagcg aacagttgga
    11221 ctactgggtt accgcagggg atactcccgc agaaatcagc gccgcctatg cacgtgttac
    11281 cggtacgccg ccaatgatgc ccgattacgc catgggattc tggcagtgca agttgcgcta
    11341 ccgtaatcag caagagttac tagaagttgc acgtggctac aagcagcgta atttgcctat
    11401 ttcggtgatt gtcatcgatt tcttccattg gccgaatcag ggtgactgga tgttcgatct
    11461 gcgcgactgg cccgatcccg atgccatgat tgccgaacta aaagagatgg gcattgaact
    11521 gatggtctcc ttctggccca cggttgataa ccgtaccgag agttatcggg agatgaaaga
    11581 aaacggctgg ctggtccaca ccgagcgtgg tttgcctatc aatatggatt tccttggcaa
    11641 caccacgttc tttgatgcta cccatcctgg cgcacgggaa tatgtctgga acaaagccaa
    11701 acgcaattat tacgataaag gcgtgaaact gttctggctg gatgaagccg agccagagtt
    11761 cggtgtttac gattatgaca actatcgtta ctacgcaggg cctgtgctgg aagtcggtaa
    11821 tatctatccg cgtatgtacg ccaaaacgtt cttcgatggt atgaccgcgg ctggtgaaaa
    11881 gcaggtgatt aacctgctgc gctgtgcctg ggctggcagt cagaaatatg gcgctctggt
    11941 gtggtcaggc gatattcact cctctttccg ttctttgcgt aatcagtttg ccgctggctt
    12001 gaacatggga attgccggga tcccgtggtg gacaaccgat attggtggtt tccacggcgg
    12061 taatattcac gatccgaaat tccatgaact gttaattcgc tggttccagt ggggggtatt
    12121 cagcccagta atgcgtttac atggcaaccg cgacccgcag gttttgcctg aacaacccta
    12181 tcgtgatggc atagcacaat gtccgactgg cgcgccgaat gaagtctgga gctatggtga
    12241 agaaaccggt gaaattctca cttcctgcct gcagttgcgt gaaaaactga aatcttatat
    12301 cagtaaaatt atggctgaga cgcacgagaa gaacagcccg ataatgcgca cgatgttctt
    12361 tgaatttcct gaccaggcag aaagctggaa tatttatgat cagtactgct ttggcccaga
    12421 ccttctcgtt gcccctgtaa tgcacgaagg tacacgttca cgtgatgtct ggttgcctgc
    12481 tggagagaca tggatcgatt tttataccca tgaacactac gcgggtgggc aaacgcttca
    12541 tcattgcgca ccgctgcacc agataccggt attcattcgc gagaaaggga aatatcgcca
    12601 gatatttccc gtttaaagga catcgtttgt tcattagcac gccccgtatg tagcggggcg
    12661 tgagttttaa tagctatcgt gaggcgggag aaaacaacga agaataatcc gtattgcaga
    12721 ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa aatagcatgg tactcttcca
    12781 tcacattctc gacgcgacca atttctacga cagttttatc ggcataaaag tcatatgcat
    12841 aaagcgttgg agcaacgaag attgcattgt gcatcgcacc aaaagctttc atcaacgcgg
    12901 catcatcaaa ctcgccaagg atttctacgt ttaatccctg ggagttaaac cagttaagca
    12961 atttgcgccc taacattgaa cgtcgcccag gaatcaaaag ccgccgttct tccagacaag
    13021 ccgggaacgg tttttccggt ggtggatttg tacaccagaa actcacgcca cattcgccaa
    13081 ttctcacgga gaacaggcct tcctgctgcg tagaatctat cggacagtcg gaaatgatca
    13141 tatccagttt atgctgactt aattgctcca gcagcatttc gtgggtggat tcgaagcagc
    13201 gaagatgaat cggctcgcct tctaccactg cggcgttaag tacgctactg accagacgtt
    13261 tggaaagtgc atctgccaca ccaacatcaa acaataagtt ggactctttg cgatagttga
    13321 cgatatccag catttcctgg cttaaggtga acattttatc ggcatagcga tagaccagtt
    13381 cccccagttc gctgggttcc agaccacgtc ctttacgctt aaatagtttc ccttgcaggc
    13441 gctcttccag cgcccggatc tgcccggtaa tggtttgtgg tgttaaataa agcgcctccg
    13501 ctgcgccaac cacagaacct tctttgtaga catgccagaa gtaatacaag tggttgtaat
    13561 tgatatgaga catgctcatt tctctccctg ataacaatga aaagggagcc gtttatggct
    13621 ccccagtaca tcgtcctgtc aaactgatgg acgtaaacga acgcgtaacc agctatatcc
    13681 aattaccgcc gaagaaattg aaccgacaag gatacctaat tttgcccagt taatcagttc
    13741 tggatctacg ctaccaaatg ccaggctggc aataaagata gacatagtaa aaccgatacc
    13801 gcacaggata ccaaccgcca taatttgctg gtaagtcgtt ccctctggca gatgtgccag
    13861 tttcaaacgc agcgccaacc agcagaacag actaataccc agtggcttgc caatcagcaa
    13921 accagcgatg atccctaatg gcagaatgga ggtcaaacct tccagcgtga caccttgcag
    13981 tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc agatacgcca cccatggatg
    14041 caaaacatgc tccagacgtt tagccggaga gcgcccatgc ttctctttta acggaataaa
    14101 gaagccgaca atgacgccag ccagcgttgc atggacacct gatttcaaca ccgctgtcca
    14161 cagcaccacg ccaaccagaa tataaacgcc cgtgcggcgt acaccacaca gattcaatac
    14221 cgcgagtacc gcaattgcta cagccgcgac gccaagagag gccatcgata agtcattagt
    14281 gtagaacaat gcgatgataa tgatggcccc aagatcgtcg ataatagcca gagccatcaa
    14341 aaagatcttc agcgctaacg gaacacgact tcccaacagc gccaacacac caagtgcaaa
    14401 ggcaatgtca gtcgccgccg ggattgccca gccttcgcga gtgataggat cggcatagtt
    14461 aaaagccaga tagagcaatg ccgggacaat catcccgccg attgcggcaa taacaggaaa
    14521 tgccgcctgg cgcagactgg ccagcgaacc ttgcatcagc tcgcgtttaa cttccagacc
    14581 aaccaacagg aaaaataccg ccatcagagc gtcattgatc catagcagca tgttcttgtt
    14641 gatctcaagt gtcccaaccc ggagctgaac cggcgtctca agaaagtcgt gataccatcc
    14701 actggttgca ccgctgttgg ccataatcat cgctaataca gcggcaataa tgagaataat
    14761 gcctcccgag gcatcactgc taaagaatcg atgcagatgt ttcacttttt atttctcttc
    14821 aggtgaatag atcgttttca cgattttacc ccgcacgaat catcgttaaa atcagattat
    14881 agtgtaggaa tagctcgctt tttacgatct attaaaagaa ttgcgccatc agcgagagat
    14941 gagtttttga tgcccgaaag cggcggctga tgaccttaaa ccccattaat catagtctaa
    15001 tgtcaagatt tctgaagaaa ttattcggaa aagtaataat taaattgttc ca
//
LOCUS       DACADA010000112        14976 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.114, whole genome shotgun sequence.
ACCESSION   DACADA010000112 DACADA010000000
VERSION     DACADA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.114"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            279..845
                     /gene="yqaB"
                     /locus_tag="ISB17_003365"
     CDS             279..845
                     /gene="yqaB"
                     /locus_tag="ISB17_003365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAP1981264.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            842..1270
                     /locus_tag="ISB17_003366"
     CDS             842..1270
                     /locus_tag="ISB17_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1981265.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1343..2899
                     /gene="gshA"
                     /locus_tag="ISB17_003367"
     CDS             1343..2899
                     /gene="gshA"
                     /locus_tag="ISB17_003367"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP1981266.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3049..3564
                     /gene="luxS"
                     /locus_tag="ISB17_003368"
     CDS             3049..3564
                     /gene="luxS"
                     /locus_tag="ISB17_003368"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP1981267.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3615..4727)
                     /locus_tag="ISB17_003369"
     CDS             complement(3615..4727)
                     /locus_tag="ISB17_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1981268.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4724..5431)
                     /locus_tag="ISB17_003370"
     CDS             complement(4724..5431)
                     /locus_tag="ISB17_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HAP1981269.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(5689..7227)
                     /gene="emrB"
                     /locus_tag="ISB17_003371"
     CDS             complement(5689..7227)
                     /gene="emrB"
                     /locus_tag="ISB17_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAP1981270.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7244..8416)
                     /gene="emrA"
                     /locus_tag="ISB17_003372"
     CDS             complement(7244..8416)
                     /gene="emrA"
                     /locus_tag="ISB17_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAP1981271.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8543..9073)
                     /gene="emrR"
                     /locus_tag="ISB17_003373"
     CDS             complement(8543..9073)
                     /gene="emrR"
                     /locus_tag="ISB17_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAP1981272.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQGGVVLEAMS"
     gene            9044..9145
                     /locus_tag="ISB17_003374"
     CDS             9044..9145
                     /locus_tag="ISB17_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981273.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(9164..9499)
                     /gene="ygaH"
                     /locus_tag="ISB17_003375"
     CDS             complement(9164..9499)
                     /gene="ygaH"
                     /locus_tag="ISB17_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAP1981274.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9489..10226)
                     /locus_tag="ISB17_003376"
     CDS             complement(9489..10226)
                     /locus_tag="ISB17_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP1981275.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10350..11534)
                     /locus_tag="ISB17_003377"
     CDS             complement(10350..11534)
                     /locus_tag="ISB17_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981276.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(11726..12718)
                     /gene="proX"
                     /locus_tag="ISB17_003378"
     CDS             complement(11726..12718)
                     /gene="proX"
                     /locus_tag="ISB17_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAP1981277.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12775..13839)
                     /gene="proW"
                     /locus_tag="ISB17_003379"
     CDS             complement(12775..13839)
                     /gene="proW"
                     /locus_tag="ISB17_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAP1981278.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13832..>14976)
                     /gene="proV"
                     /locus_tag="ISB17_003380"
     CDS             complement(13832..>14976)
                     /gene="proV"
                     /locus_tag="ISB17_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAP1981279.1"
                     /translation="AFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSG
                     SGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDVELREVRRKKIAMVFQSFALMPHMT
                     VLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALA
                     INPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIM
                     QNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGP
                     RSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQT
                     PLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3677 a         3862 c         3987 g         3450 t
ORIGIN      
        1 ttctccgtaa ccttcagcaa tgaaggtatc gaagaggtag cggtattaac cgcaggcacc
       61 agggaggata acgttgcgcc agcagcggcc cgaagggcga acgccagtga gtcatcctcc
      121 cggatgcacc atattatcca aaaaatttcc ttcaaaaata tctcatttct aaataactac
      181 tttatccatt tcctgaattt tcttctcctt ctccatcagc gacaaaatca agcaattacg
      241 ctaaagtagc gtcatatttt tcgacttgcg agaagaccat gtacgagcgt tatgcaggtt
      301 taatttttga tatggatggc accatcctgg ataccgagcc tacgcaccgt aaagcgtggc
      361 gcgaagtatt agggcactac ggtcttcagt acgatgttca ggcgatgatt gcgcttaatg
      421 gctcgcccac ctggcgtatt gctcaggcga ttattgagct gaatcaggcc gatctcgacc
      481 cgcatgcgtt agcgcgtgaa aaaacagaag cagtaagaag tatgctgctg gatagtgtcg
      541 agccgcttcc tcttgttgaa gtggtgaaaa gctggcatgg tcgtcgccca atggctgtag
      601 gaacggggag tgaaagcgcc atcgctgagg cattgctggc gcacctggga ttacgccgtt
      661 attttgacgc cgtcgtcgct gccgatcacg tcaaacacca taaacccgcg ccagacacat
      721 ttttgttgtg cgcgcagcgt atgggcgtgc aaccgacgca gtgtgtggtc tttgaagatg
      781 ccgatttcgg tattcaggcg gcccgtgcag caggcatgga cgccgtggat gttcgcttgc
      841 tgtgagtgaa gcgttatcgc ttttctcgtt gtttgccagt agttttctca gcgctacact
      901 attacccggc aactccgaag tcgtgctggt ggcaatgttg ctttccggag tgagtcatcc
      961 ctgggtttta gttttaacag caacaatggg taatagcctt ggagggttaa ctaacgttat
     1021 ccttgggcgt ttctttccat tgcgtaaaac atcgcgctgg caagagaaag ctaccggctg
     1081 gctgaaacgc tatggtgcag tcacgctatt attaagctgg atgcccgtgg ttggcgattt
     1141 actgtgtctg ttagcgggat ggatgcgcat ctcgtgggga ccggtaatct tttttttgtg
     1201 ccttggtaaa gcgttacgct atgttgcagt tgcagcagct accgttcagg gcatgatgtg
     1261 gtggcactaa ttgtaggcct gcacatatgg tcaccattac agttatgcta attaaaacga
     1321 ttttgacagg cgggaggtca atttgatccc ggacgtatca caggcgctgg cctggctgga
     1381 aaaacatcct caggcgttaa aggggataca gcgtgggctg gagcgcgaaa ctttgcgtgt
     1441 taatgctgat ggcacactgg caacaacagg tcatcctgaa gcattaggtt ctgcactgac
     1501 ccataaatgg attactaccg attttgcgga agcattgctg gaatttatta caccagtgga
     1561 tggtgatatt gaacatatgc tgacctttat gcgcgatctg catcgctata cggcgcgcaa
     1621 tatgggcgat gagcggatgt ggccgttaag tatgccatgc tacatcgcag aaggtcagga
     1681 catcgaactg gcacagtacg gcacttctaa caccggtcgc tttaaaacgc tgtaccgtga
     1741 agggctgaaa aatcgctacg gcgcgctgat gcaaaccatt tccggcgtgc actataattt
     1801 ctctttgcca atggcattct ggcaggcgaa gtgcggtgat atctcgggcg ctgatgccaa
     1861 agagaaaatt tctgcgggtt atttccgcgt tatccgtaac tactatcgct tcggttgggt
     1921 cattccttat ctgtttggtg cgtctccagc gatttgttct tctttcttgc aaggaaaacc
     1981 aacgtcgctg ccgtttgaga aaaccgagtg cggtatgtat tacctgccgt atgcgacctc
     2041 tcttcgtttg agcgatctcg gctataccaa taaatcgcaa agcaatcttg gtatcacctt
     2101 caatgatctc tacgagtacg tggcgggcct taaacaggca atcaaaacgc catcggaaga
     2161 gtacgcgaag attggtattg agaaagacgg taagcggctg caaatcaaca gcaatgtgct
     2221 gcagattgaa aacgaactgt acgcgccgat tcgtccaaaa cgcgttaccc gcagtggtga
     2281 gtcgccttct gatgcgctgt tacgtggcgg cattgaatat attgaagtcc gctcgctgga
     2341 catcaacccg ttctcgccga ttggtgtaga tgaacaacag gtgcgattcc tcgacctgtt
     2401 tatggtctgg tgtgcgctgg ctgacgcacc ggaaatgagc agtagcgaac ttgcctgtac
     2461 acgcgttaac tggaaccggg tgatcctcga aggtcgcaaa ccgggcctga cgctgggtat
     2521 cggctgcgaa accgcgcagt tcccgttacc gcaggtgggt aaagatctgt tccgcgatct
     2581 gaaacgcgtc gcgcaaacgc tggatagcat taacggcggc gaagcgtatc agaaagtgtg
     2641 tgatgaactg gtcgcctgct tcgataatcc cgatctgact ttctctgccc gtatcttaag
     2701 gtctatgatt gatactggta ttggcggaac aggcaaagcg tttgctgaag cgtaccgtaa
     2761 tctgctgcgt gaagagccgc tggaaattct gcgcgaagag gattttgtcg ccgagcgcga
     2821 ggcgtctgaa cgccgtcagc aggaaatgga agccgctgat accgaaccgt ttgcggtgtg
     2881 gctggaaaag cacgcctgac agaaaagaaa aaggccactc gtgagtggcc aaaatttcat
     2941 ctctgaattc agggatgatg ataacaaatg cgcgtctttc atatactcag actcgcctgg
     3001 gaacaaagag ttcagaaaat tttaaaaaaa attaccggag gtggctaaat gccgttgtta
     3061 gatagcttca cagtcgatca tacccggatg gaagcgcctg cagttcgggt ggcgaaaaca
     3121 atgaacaccc cgcatggcga cgcaatcacc gtgttcgatc tgcgcttctg cgtgccgaac
     3181 aaagaagtga tgccagaaag agggatccat accctggagc acctgtttgc tggttttatg
     3241 cgtaaccatc ttaacggtaa tggcgtagag attatcgata tctcgccaat gggctgccgc
     3301 accggttttt atatgagtct gattggtacg ccagatgagc agcgtgtcgc tgatgcctgg
     3361 aaagcggcaa tggaagacgt gctgaaagtg caggatcaga atcagatccc ggaactgaac
     3421 gtctaccagt gtggcactta ccagatgcac tcgttgcagg aagcgcagga tattgcgcgt
     3481 aacattctgg aacgtgacgt gcgcatcaac agcaacgaag aactggcgct gccgaaagag
     3541 aagttgcagg aactgcacat ttagtcagtt taagcagaag aggccgggct ggtgaaaaca
     3601 ccgcccggct tttgttacct gtcagtcatc cgatattcca cgctatgtgc ttcatcaggt
     3661 tgaggaactt ccagccttga ggccatccgc atcacgaccg cttcaggaac tgcatactcg
     3721 cgtctggcat tttgctggtt ccattgcgcc catggcactt ccagataaac gatttttact
     3781 cgtgcgccgt aagccgtaaa cagactgatg agctggctgc gcagctggcg ggtaatgttc
     3841 gttgcattcc agatgaacgg ctttttctgg cgtaaaaaaa cgcgcgcctc ttcttttgcc
     3901 tgttgcacaa tacgcccggt ggcggttttg tcgtccggac tggcattgat tcgccgccgc
     3961 atatcatcaa ggctgataac gtccattccc tggcactgtt cactgatata gcgatctttg
     4021 cccatacccg gtaagccaca cagcaaaata acctcaaaag gttcagcctc ccagggaaca
     4081 aaatcaggag aactttgctc atgagtcagg tagtgccagc gtgcggaatc agaaacgaac
     4141 gggcgcattt ttccccagca ttgttgctca tggcaaaaca gctcgaacag atcgatgcgt
     4201 tccagcattg attgctgatc cgggctttgg cgaccaagca gatcggcacg agcaagcaaa
     4261 gcgagcagac gcgtgtcgat gcgcattgca gccgtgagca acaaacgctc cggttccgga
     4321 cgttccagta accatagcgg taacccatgc agacgcacca gtgcaacaat ttgctcgcgc
     4381 agcacgaacg gcgttgggat atcgcgccat aaaatttgtc gagccgtcag ttcgccccga
     4441 cgagcatgac caggagactg aatacgtccg ttttcctgca cggtagtgct gcgcttttca
     4501 acatcatgca ataacgccgc cgcccataaa acttcctgct gttgagcggg gagttgctgg
     4561 aattctggca gagtaatgag ggcgttaagc accatttcgg tatgtacgcc gacgtcgcct
     4621 tcaccatgat gctcagggtc ttgtggcgtg tgatgcatct cctcaaccca gctaaaacgc
     4681 tgacgtagcg ccgaccagcg tttatcgtcg gtaagttgcc aaatcatgac tgttctccat
     4741 aaacgaactc atgggccatc cgcgcccgtt gccagtggcg tttccagtgc acagtggttt
     4801 tgacatgatt tttgcgcaca tacttgaaga cgttatgcga aaagtcagcg acagaaaacg
     4861 catcatgatt tcggctgaca atgccttcca gcgtacaggg ttgacaggtt tgcgtatccc
     4921 agggatcgaa tgtgccgcgc gcattggtca gcgccagaaa atcacgttgc cagctttttt
     4981 catcattccc cggctgcggg ccggagattt cgggaacgca ggggaaatca aacagagcgg
     5041 cgtaaaactg cacctcttcc cagctcagcc acatatcctg gcagcgaacg gcaaacaggt
     5101 aaaagtcctg ttccagcgcg cggtattcaa tggaatgaac ggcgtagaga ttttcaccaa
     5161 acaactccag gttgccgaga tcgtttttca gcagttgcca gcgttggcga attttatagg
     5221 tccatgccga ctgcgtaggc gtggcatggg agcgggcaaa cacgccgtag cggttaaggc
     5281 agttattttc gccatcgagt ttttctgtgt gcaccaattg cgtgatggtc tgtaaatcct
     5341 gccagtaatc ggtgttaatg cggtcatcgc tggtggtgcc tggagagaag gggtaatgcc
     5401 aggttctccc gtatttacgt tgggtattca taataatcca ttattttgtg tgtaataaat
     5461 gtacgcccgc gagggtgcga gttttggggt taaaagaaaa atgtgagtat cacccgcgaa
     5521 agcgagggcg aaggaaaaag agaattaaca acctgagcga atgctcagaa ccggctaatg
     5581 gtgaatggaa gagtttgggg cggcgaaaag atacaccgaa tgagtgtata aaacaaccgt
     5641 ttgcagaatg agcaaataaa aagggagccg cagctccctt tggcaacctt agtgcgcacc
     5701 gcctccgccg ccacctgcgc caaatggcgg tttagcaaac cacaccagcc ccagcaggac
     5761 gaggaatatc ccggctgaca tccagaagat ctcattggcg gaaataatca gcccctgatt
     5821 ggttatctgc tgggcaatcc agcctgatgc ctgctgttgc gtcataccaa gcccttccag
     5881 ttgactgtac atcgcctgag cattcgggtt gaacgggttt accgactcag tcaactgtgc
     5941 atggtgcagc gactcgcggt tggtccacat ggtcgtggtt atcgacgtgc cgatagaccc
     6001 cgccagcgtt cgcgtaaagt tggagaggct cgatgccgcc gccagtcgtt ccggtggcaa
     6061 accggacagc gtaatggtgg tcagcggcat aaagaagcag gccaccgcaa aaccctggat
     6121 aaactgtggc caggccgacg cgccaaaatc catacccggt tcaaaggtat aggcacgcca
     6181 gtagaagcag acggcataca taataaagct gaaggttacc agtcggcgca tatccagttt
     6241 atgcgcgaag cggccgataa tcggcgacag gatcaccgga ataatcccta ccggcgcaga
     6301 ggccagccct gcccacgtcg ccgtgtaacc gtagacctcc tgcaacaact gcggcagcag
     6361 aacaatcgcg ccgaagtaga gcatatacgc gaggctgata cacaagcagc cgatggtgaa
     6421 gttgcgcgac ttaaacaacg acagatcgac tatcgggtta tcgtcggtca gctcccagac
     6481 aatcaggaag cagatagcca ccaccgccac cacggtgagg atgataattt cctgtgatga
     6541 gaaccagtcc agctctttac cgcggtcgag cataatctgc aggctgccga taccaataac
     6601 cagcagtgcc agccccacgg catcaatccg ccgccgttcg gtgcgggttt cacgtccgcg
     6661 cagagtttgc agtgtcatca acaccaccgc cacgccaatc ggcacgttga tgaagaatat
     6721 ccagccccag tggtaattat cactgatata accgccgagg atcgggccgc aaattggcgc
     6781 aacaatcacc gtcatcgacc acaacgccag cgcgatcgag cgtttggctg gcgggtagtt
     6841 attcagcaat agactttgcg aaagcggaat taacggcccg gcgacaatcc cctgaatcac
     6901 gcggaagaag atcagcatat tcaggctgct ggagacacca cacgcccacg acgcaatagc
     6961 aaaggcgatg gtggaccaga ggaacagttt cacttccccg acgcgctttg ccagccagcc
     7021 ggtaagcggg atcgagatgg cattcgccac cccgaaagaa gtgattaccc acgttccctg
     7081 gctgagcgat gagcccagat tcccggcgat agtggggatc gccacgttag caatggtgga
     7141 gtccagcacc tgcatgaatg tcgccagtga cagcgcaatc gtcataatga ccagttgtgc
     7201 gccttccagc ggtttttgct gttgcatcac acgcacctct ggattagcca gcgttagctt
     7261 ttacgatatc gtcgatcagt ttattgacag gtgccaggct gatttcacgc gcggtgctta
     7321 ccgcgaccgg tgtggaacgt actttgtttg ccagtacctg accgtcacgg ttagtggtat
     7381 tgacactcac cagcgtggat aaaccgatac gcagcgggta ttgctccagc tgtttctggt
     7441 ccagttcaat acgcacaggc agacgctgaa cgactttgat ccagttaccg gtggcgtttt
     7501 gcgccggaag cagtgagaac gcgctacctg tgcccatatc cagaccaacc actttaccgg
     7561 tgtatttcac atcatcgccg taaatatcgg tggtgatggt gaccggttga ccgatacgca
     7621 tattggcaat ctgcgtctct ttaaagttgg catccaccca catattggtg gctggaacga
     7681 ccgccatcag cggcgtcgtt gggctaattt gcgccccagg ctgtaccgcg cggcgggaga
     7741 cataaccggt catcggactg acaatacggg tacgctccag cgccagccag gcgttacgta
     7801 cttcggtggc agcctgttgt acggcaggct gatcttccag tttcgtcccc agaatcatcg
     7861 cctgattggc attgtattgt tgaatagcga cgtccagttg cgcctgggca ctggtgacgg
     7921 cgtcgcgggc gtgttgcagc tcttcgcgac caatcaggtt ggcattgccc agcggcacac
     7981 ggcggttgta gtcgctttgt gctttcgcga gggcgatttt ttgcacctca atattcgcct
     8041 gcaactgttt gctgttaatc atcagctggt gggtttggcg aacgctggaa gccagtgcag
     8101 ttttggcttt ttcaaacgcc tggcgagcat ctgtcgggtc gagagtgacc agcacgtcgc
     8161 cttcttttac aaaatcggtg ttatcggccc agactttcgt cacgctgcca gacacctgag
     8221 acataatttg aatctgattc cctgccacgt atgcgtcatc ggtttcttcg aaatgacgca
     8281 gtaccaaaaa ccaataaatc cctatcgcta cggcaataat tataaagagc aaggtgagaa
     8341 ggaggagcag acgcttacgt ttgccgctct tctttaccgg ttgctgcggg gtttgagtct
     8401 ccgcatttgc gctcatattg ttctccacga tcttcttaac tcatcggctg agccgacctg
     8461 cttgccaaaa aggccagcat gttgcgatgc tggccggtta ttttttcttt tataaatctg
     8521 gatttttgag cgagatgacg cgttagctca tcgcttcgag aaccacaccg ccctgttcca
     8581 tctggtcgag acgggagagc aatttgcggg tgatttgctc gagctggtct ttttctgttg
     8641 tgctgagcgc ggaccagagt tgatgcaggc agttatgctg cggcggtaaa acctcgcgca
     8701 aaaactcgtg acctttttcc gttaattgca gatgcaggca gcggcgatcg ttatcgcttt
     8761 cacgacgttc gatccaaccg cgtttttcca gttcatcggc aatacgcgtc gcgttggtac
     8821 gggatgatcc aagagcacaa ctcaattcag aaggctgaat actgtggttt tcctgagact
     8881 ccagcgtaat caacgccata aacaacgtct cgttaatccc ctgagccttc agcattttat
     8941 tgcggttttc cagcagcttg ctttgcatat gcatgcaaag acgggtcaga aggatctcct
     9001 gataaggaaa atcttcgtgg cggctggcgc gaaattttag catttgttca atgggcgtaa
     9061 acgaactatc catttgggta tgacctcatt aatttcagcc gatatagtaa cgacagtgac
     9121 aaataaagta aatgtattgt tttagaaaaa tgattcttgt ggattatata atcgccatca
     9181 ctttccaggc gagcccatag gccagcgcac taagcagtgt tgggatgata atgctgcgtg
     9241 ttttatagaa actggcaccc agtaccgcga agccgaccag tgtgggcacg aaacggcgtg
     9301 tatcgtgcat cacttctggt gcggtagaga caaccagcag agcgcatatc gaggcgatgc
     9361 caatggtgtc gagcaaaata cctaccgcgc cacgtttggt tgggcgggca ttacccacac
     9421 gcaggcgcag cggcagatag cggaagcaat aattcgccgc gccaaccagt aacccaagca
     9481 gcagaacctc atagctcatc gggcgctcct tgccagaatg cctggattaa cgcagtgagg
     9541 cagccacaga caatgcctgc cagaatggcg acgggaatag aaaatagcgt tacgcctgca
     9601 agggcaccaa ctaacgctgc ggtaacgcaa agagattgtt tgcgctggaa agaggcgagc
     9661 aggaaactca taaagagtgc cggaagcata aaacctaatg ctgcttcaac ggcgggataa
     9721 ccttgcagca agccgctgcc ggagaatgcc cctattaccg taccaaatac ccacgatgac
     9781 catgaactga aggcaatgcc gatcatccag ttttcgctcc agcggcgatt attgcgtacc
     9841 agttttgcgg ttgcggcggc aaaaacctca tccgtcaggc caaacgccca cagggcggtt
     9901 ttcgattttt gcagacgctg aataatacgg ctacgcagtg acgggccata caaaacatgg
     9961 cgaacatcca ttgccatgac ggtcagtgca gcaacccaca aactactccc ggctgccagc
    10021 atcgcggtaa tgacgaactg gctcgcgcct gcataaatga tgcaggagaa aaaaacgctt
    10081 tcgagaggag agaatcccag acgggtcgca ttcagaccga acgcaaaggc caccggaata
    10141 taactaataa caatcggtaa actgtctttg catccttcca tgaaggtcgc cgaaccagga
    10201 gcaggctgtg gagtagggct ttccatagag tgtacgctta acgattgtta atattataaa
    10261 tagactgaat gaatatctta accttatcag actgatgggc ttcttaacac ccttataagt
    10321 gtaaagccac gaaaacggtt gctgattgct taatttgcgg cttcctgacg atgaaaacct
    10381 cgccaccaga ccagtaagtt aaccagggca atcgtcgcgc cagccagaca cacgccagcc
    10441 caactgccat gttgccaggc tgaggctgaa attagcgaac cagcggcacc gccaataaag
    10501 tagctggtca tgtaacctgc ggtcaggcga ttacgcgcat cagggtgtat tcgataaatt
    10561 accgtctggt tagtgatatg cacgccctgc acggtgaggt ccagcaccag gatgccgata
    10621 atcagcgcca gtacggaagt gtgaccaaac cagattgcca gccatgaaag taacagcaga
    10681 agcagaccga aagttgttgt gtggtgcgat ttgcccttgt cggcaaaacc gcccgccgga
    10741 cgagcgccca acgctccggc agctcccgca agtccaaaca gaccaattac accatcgctg
    10801 tagttaaaag gtggagcggc aagcaaaaag gccattgagg tccagagaat gctgaagttg
    10861 gcaaaggtca ggcagcccag caacgcgcgg gtgcgcagga tcttatcgct gataaacatg
    10921 ctgaaaacgg aaccaagcaa ctgtgggtag ttgaggtggg tttctgattt catttgtggc
    10981 agaccacgcc ataatgccag cgccatcagt gccattaaca ccgaggcaac ccaaaagacg
    11041 gtgcgccagc cgccgaggtt tgccagcaat ccggcaacgg tccgtgcgag taagatcccc
    11101 aatagcaggc cgctcataat ggtgccaacc actttgccgc gtttgtccgg tgaggccagc
    11161 gtcgctgcca gtggaaccag aatttgtgcc acgaccgaga ataaaccggt taatgccgta
    11221 ccgaggatca tcatcgccag tgactgactg ctggcagtga ttaacatacc gcccgctgcc
    11281 agtaaggtca tcgagacaat caggcggcgg cgttcaaaca tatcaccgag gggaacaaga
    11341 aacagcagac ctgcggcata gcccaactgc gcggcggtaa caataaagcc cgccgaactg
    11401 gcggaaaggg aaaagttacg cgcgatggtg tcgagcaatg gctgggcgta atagttactg
    11461 gctaccgcca gaccggtggc gatagacatc agcacgatca gcgccgggct aagctcatga
    11521 ttaggtttag tcattatttt tgttcatcag gtgatgatta agctggaaat caataatagc
    11581 ggatgttgat gaatatggga tgaaagatag attgttgaaa tgtgcggtgt ggtttgtagg
    11641 tatgataaga cgcgccagcg tcgcatcagg cattgtgtac agagcggcgg atgaggcaaa
    11701 aacgccttat ccgcccgaat aaaaattact tctgcgctgc cagcgcttca ttcacccagc
    11761 catcgaactg ctgctggtgg gctttgatcc aaccatcaac gtggccctga atatcgtctt
    11821 ctgaggcttt gccgtcatgc atgatggcgt tctgggcgtt aatatctgcc actggtaact
    11881 gcataatggc aaacagtttc gctgctgccg ggtttttctc ggcccaggct ttgttggcaa
    11941 cgatatgcat ggtactgacc gggaagccgt aattcgcgcc attcggcagt ttggtattgg
    12001 catttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca tctttccccg
    12061 gtttcagttc gttactcacc cagtacggcg tccaggtgta gtaaaacacc ggcttaccct
    12121 ctttgtaacg actgatggtg tcagccatca tcgctgcgta gttcccctga ttatgcgtca
    12181 cggtattggt cagtccatac gcggcaagct ggtggttgat cgcaccttcg cagccccagc
    12241 ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac agtttggcga
    12301 tcttcggatc tttaagttgc gcgatgttgg taattttata ctggtcggcg gttttcttat
    12361 cgatcaggta accttgtgcc gcgccgttaa caaatacccc ttcacgataa aatttcttat
    12421 cgccaccggc agcttcgtac atgttgtcat gcagtggcgt ccagttcacg gcggtgaagg
    12481 ttgcatcgcc ggaagcaagc gaggtgtagc caacgttgta atccacttcg ctgggtttat
    12541 tgacggtata acctaatttc tccagcgcgc ggctgaccag cagcgtctgg aaggtttctt
    12601 cggtgatggt gctttgaact ggattaactg taatgccttt gcccggcagg tcggcagcaa
    12661 aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta tgtcgcattg
    12721 ttattccttt ttttggcagg gtgatatgcc cggtgttacc gggcaagtgc agagttactt
    12781 aatgaatggg cgggtcagca gaccgacagg gccagtggtg taccagcgac ggttgccgcg
    12841 actacgtgag tcgcgcccaa cggcctgcgt caggcgatcg aggataatgg cgaggatcac
    12901 aatcccgacg ccgccaacgg tggcaagccc catatccaga cgaccgatac cgcgaagtac
    12961 catctgaccc agcccgccga cggcaatcat cgaagcgatg accaccatag aaagggccag
    13021 catcagcgtc tggttaacgc ccgccataat ggttggcatc gccagcggta actgaacttt
    13081 gaacagcatc tggctcgggc tggcaccgaa tgagcgcgag gcttcaatca gatccgccgg
    13141 aacctggtta attcccagaa tcgtcagacg gataatcggc ggcagtgcaa agatgatcgt
    13201 caccaccacg cccggcacgt tgccgatacc aaacagcatg acgattggca ccagataaac
    13261 aaacgcgggc gtggtctgca tggcatcaag cagtggacga ataattttcg ccgctcgcgg
    13321 acttctcgcc agccagatcc ccaacggcaa acctatgacg atacagaaca gcagggcggt
    13381 taacaccagc gccagggtaa ccattgcctg tgaccaggca ccgattgcgc cgatggcaat
    13441 cagcgaaacc agcgtcgcca cgcccattcc gaccccggaa atctgccagg cgatgagagc
    13501 gaaaacgata atcgccaccg gcgcgggcat acccagcagc aattgctgga aaccgttgag
    13561 gatgtaatca accggaacgc gcacgccctg aaagacggga cgaaaatggg taacgaccca
    13621 gtcgatccct tcagtgaccc aactgtcgag cgggatcaga gttttatgga acggatcgag
    13681 aatattaaaa tgctcgacgt ttggcgcagg cgtactggtc agccagtcag caccaccgcc
    13741 gtcagtcggt gcagcagccg gtgtacccca ggcgtctgcg gattgtgcag cactgtccgc
    13801 cgctggcgtg gtatcccacg gattattttg atcagccatt atttaccccc tcacgatcta
    13861 aagcgcgcag cagcattcct ttcgaaatga tgccgacata ctgttggtcc tcgtcgacca
    13921 cgggcaccgc acagggtgcc tgtccgacat gagagagcaa ctcgctaaga ggcgtttgtg
    13981 catcgaccgc taacggcgca tcaatcagcg ccgcatcaag accttgctgc tgcgttaacg
    14041 cggctttaag cgaatcgata gacacagcgc cgacaaactt attaccgcgt tcgatgacgt
    14101 agccatattc gcgatcttca tcctgcaata atttcagtgc cgaacgcggg ccgaagccgg
    14161 gggttttacg aattaagccg ttcggtgtac gacgagcaat atctttcgca ctgaacacct
    14221 ggctaatatc gacgccacgg aagaaggtac gaacataatc attcgccgga ttattaagaa
    14281 tttcatccgg tgtaccaacc tgcaccactt caccattttg cataatggca attcggtcac
    14341 caatacgcat agcttcatcg agatcgtggg aaataaagac aatggtgcgc tgatgtttcg
    14401 cctgtaattt taccagctca tcctgcatct cggtgcgaat taatggatcg agtgccgaga
    14461 aggcttcgtc cattaataat atatccggat taatcgctaa cgcgcgggct aatcccacac
    14521 gttgacgcat cccgccagag agttcatccg ggtagctgtg ggcataattt tccagcccga
    14581 cctgacgcag tgcatcaagg gctttttccc ggcgttcttc ggcattaatt ccggccagtt
    14641 ccataccgaa cgcagtattg tccagcacgg tcatatgcgg cattaaggca aaggactgga
    14701 agaccatcgc aatctttttt ctgcgcacct cacggagttc gacgtcggat attttggcaa
    14761 tatccacacc atcaatcagc acttgcccgc gggtgggttc aatcaggcga ttgagaaggc
    14821 gtaccattgt ggatttaccc gaaccggata atcccatgat gacaaatatc tcgccttctt
    14881 caatggccag actggcgtct tttacgccaa gcgatagccc agttttttcc agaatttgtt
    14941 cttttgaaag tccttgttcg atatatttga aagctc
//
LOCUS       DACADA010000113        14948 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.115, whole genome shotgun sequence.
ACCESSION   DACADA010000113 DACADA010000000
VERSION     DACADA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.115"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(61..2340)
                     /gene="maeB"
                     /locus_tag="ISB17_003381"
     CDS             complement(61..2340)
                     /gene="maeB"
                     /locus_tag="ISB17_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAP1981280.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2629..3579
                     /gene="tal"
                     /locus_tag="ISB17_003382"
     CDS             2629..3579
                     /gene="tal"
                     /locus_tag="ISB17_003382"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP1981281.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILNIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3599..5602
                     /gene="tkt"
                     /locus_tag="ISB17_003383"
     CDS             3599..5602
                     /gene="tkt"
                     /locus_tag="ISB17_003383"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1981282.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5679..6722)
                     /locus_tag="ISB17_003384"
     CDS             complement(5679..6722)
                     /locus_tag="ISB17_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAP1981283.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6848..7423)
                     /gene="nudK"
                     /locus_tag="ISB17_003385"
     CDS             complement(6848..7423)
                     /gene="nudK"
                     /locus_tag="ISB17_003385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAP1981284.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7491..9470)
                     /gene="aegA"
                     /locus_tag="ISB17_003386"
     CDS             complement(7491..9470)
                     /gene="aegA"
                     /locus_tag="ISB17_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAP1981285.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9676..11376
                     /gene="narQ"
                     /locus_tag="ISB17_003387"
     CDS             9676..11376
                     /gene="narQ"
                     /locus_tag="ISB17_003387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAP1981286.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11540..14653
                     /gene="acrD"
                     /locus_tag="ISB17_003388"
     CDS             11540..14653
                     /gene="acrD"
                     /locus_tag="ISB17_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAP1981287.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14752..14811)
                     /gene="ypfM"
                     /locus_tag="ISB17_003389"
     CDS             complement(14752..14811)
                     /gene="ypfM"
                     /locus_tag="ISB17_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAP1981288.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3490 a         3973 c         3914 g         3571 t
ORIGIN      
        1 atattgcagc taaaccgctt accctgaata ttcagggtaa gcgtgagagt taaaaaagaa
       61 ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc accatgttga cgatacgacg
      121 taccgatgcg atcggcgtta acacgtgaac cggcttcgcc acccccatca gcaccgggcc
      181 gacagtcaca ccttccgagc tggaaacacg cagtaagttg taactaatgc gggcagcttc
      241 catgttcggc atcaccagaa tattagcgga acctttcaaa gggctgtccg gcatacggtc
      301 gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt tcaccatcaa tcatcagttc
      361 tggcgcacgt tccctgacca gttccagcgc ctgacgcatt ttgctcgacg acgggcagtc
      421 agaagaacca aagttggagt gcgacaacag agccacacgc ggctcaatac caaaacgacg
      481 aacagtttct gccgccatca aggtgatctc cgccagctct tctgcatcag gctcatcatt
      541 gacgtaggta tcggcaataa aggtgttacc actcggcaac agcagcgcgt tcattgcacc
      601 tgcggtgtga acgccatcgc gataaccaaa gacatttttc accacgctaa agtgttcatg
      661 ataatcgccc accgtaccgc aaatcattgc atcggcttcg ccacgctgaa ccatgatcgc
      721 gccgatcact gtcgggttac tgatcagcgc acgctgcgcc tgctcctgag tgacaccgcg
      781 acgcttcatg atctggaagt attcggtcca gtactcctta aagcgcggat cggattcgtt
      841 attgacgatc tcaaaatcaa cgcccgcctt gatctgcaag cccagtttct gaatgcgcat
      901 ttcgatcacg ttcggacgac caataaggat cggtttcgcc agtcccagcg ttaccagttc
      961 ctgagtggca tgtagaacgc gcgcctcttc cccttctggc agaactacgc gcttcggcgc
     1021 tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg gttttgtaaa cgaactccgt
     1081 cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc gtcgccacgc ccgactccat
     1141 cgccgctttc gccaccgcag gagcgatctt aacgatcaag cgcggatcaa acggttttgg
     1201 aatgatgtat tccggaccaa agctcagatc ctgatcgcca tacgctgaag ccaccacttc
     1261 actctgttcc gcatgggcca gttctgcaat cgcgcgtacc gccgccagtt tcatctcttc
     1321 gttgatggcg gttgcgccaa cgtccagcgc gccacggaag ataaacggga agcaaaggac
     1381 gttgttcacc tggttcgggt agtcggaacg accggtacag atgatggcat ccggacgcac
     1441 ttctttcgcc agcggcggca gaatttccgg ttccgggttc gccagcgcca ggatcattgg
     1501 cgcacgggcc attttcttca ccatttcctg ggtcagcact ttcgggccgg aacagccgag
     1561 gaaaatatcc gcgccttcaa tcacatcatc gagggttcgt ttgccgtcat ccaccaccgc
     1621 atatgcggct ttggtttccg ccatgtttgg ctcacggccc tgatagataa cgccttttga
     1681 atcgcaaacc acgatgttat gtttttgcag acccagcgct accagcaggt tcatacaggc
     1741 gattgctgcg gcacccgcgc cagaaaccac catccgcacg tcggagatgt ttttctccac
     1801 cacgcgcaag ccgttgagga tggcggcagt gctgataatt gccgtgccgt gctgatcgtc
     1861 gtggaatacc ggaatattca tccgctcgcg cagtttctgt tcaatataga aacattctgg
     1921 cgctttaatg tcttcgaggt tgatgccgcc gaaggtcggt tcgagcgcag cgacgacttc
     1981 aataaatttg tccgggtcga gttcatcaac ttcaatgtca aatacatcaa tcccggcgaa
     2041 tttcttaaac agaacgccct tgccttccat caccggtttg cctgccagcg cgccaatgtt
     2101 gcctaacccc agcaccgccg taccgttaga gatcaccgct accaggttac cgcgcgcggt
     2161 atatttgtag gcttttaacg ggtctttttc gatttcaaga caaggtgcgg caacgcctgg
     2221 tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc ttggttggag aaacctggat
     2281 tttccctgga actggaaatt catggaaatc aagtgcactt tgttttaact ggtcatccat
     2341 ttggttgttc ctttcacgta acgttcacaa ataaattgtg tgggcaacag cccctgccca
     2401 caacgtggcg cacattatta ccctgccgga gtctacagac tttgagcaag tccaaactct
     2461 caccattaat ataatgtttt ggtaataatc atataacact gatgttacct gcttaatcca
     2521 gcaataccat gcctgtctgc tatgcttttt tgatgcgttt agcgaaattt ctcagaagtg
     2581 tgaattaacg cactcatcta acactttact tttcaaggag tatttcctat gaacgagtta
     2641 gacggcatca aacagttcac cactgtcgtg gcagacagcg gcgatattga gtccattcgc
     2701 cattatcatc cccaggatgc caccaccaat ccttcgctgt tactcaaggc tgccggatta
     2761 tcacaatatg agcatttaat agacgatgct atcgcctggg gtaaaaaaaa tggcaagacc
     2821 caggaacaac aggtggtcgc agcgtgtgac aaactggcgg tcaatttcgg tgctgaaatt
     2881 ctcaatatcg tacccggtcg cgtgtcaaca gaagttgatg cacgcctctc ttttgataaa
     2941 gaaaagagta ttgagaaggc gcgccatctg gtggatttgt atcagcaaca aggcgttgag
     3001 aaatcgcgca ttctgatcaa gctggcttcg acctgggaag gaattcgcgc ggcagaagag
     3061 ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt tttcttttgc acaggcgcgg
     3121 gcctgtgcag aagcaggcgt ttttctgatt tcgccatttg tcgggcgtat ttatgactgg
     3181 tatcaggcac gcaagccgat ggacccgtat gtggtggaag aagatccagg cgttaaatca
     3241 gtacgcaata tctacgacta ctataagcaa caccactatg aaaccattgt gatgggcgcg
     3301 agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct gcgaccgact gactatcgca
     3361 ccgaatttgc tgaaggagct acaggaaaaa gtttcgccag tggtacgtaa attaatccca
     3421 ccttctcaga cgttcccacg cccagctccc atgagcgaag cagagttccg ttgggagcac
     3481 aatcaggatg cgatggcggt agaaaaactg tctgaaggca ttcgtctgtt cgccgttgat
     3541 caacgcaaac tggaagatct tcttgccgcc aaactataaa ccagccacgg agtgttatat
     3601 gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt atggatgcgg tacaaaaagc
     3661 caattctggt catcccggcg cgccgatggg catggctgat attgccgaag tgctgtggaa
     3721 cgattttctt aaacataacc ctaccgaccc aacctggtat gatcgcgacc gctttattct
     3781 ttccaacggt cacgcgtcga tgctgctcta cagtttgctg catctgaccg gttacgacct
     3841 gccactggaa gaactgaaga acttccgtca gttgcattcg aaaacccctg gtcacccgga
     3901 aatcggttat acgccaggtg ttgaaaccac caccggtcct cttggacaag gtttggcgaa
     3961 cgccgtcggg ctggcgatag cagagcgtac gctggcggcg caatttaatc agccggatca
     4021 tgaaattgtc gatcacttca cctatgtgtt tatgggcgac ggctgcctga tggaaggtat
     4081 ctctcacgaa gtctgttcgc tggcgggcac gctggggctg ggcaagctga ttggttttta
     4141 cgatcacaac ggtatttcca tcgacggtga aaccgaaggc tggtttaccg acgatacggc
     4201 aaaacgtttt gaagcctatc actggcatgt ggtccacgag atcgacggcc acgatccgca
     4261 ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa gataagccgt cgctgattat
     4321 ctgccgtacg gtgattggct ttggttcgcc gaataaagca ggtaaggaag aggcgcacgg
     4381 cgcaccgctg ggggaagaag aagtagctct ggcacggcaa aaactgggct ggcaccatcc
     4441 gccatttgag atccctaaag agatttatca cgcctgggat gcccgcgaaa aaggcgaaaa
     4501 agcgcagcag agctggaatg agaagtttgc cgcctataaa aaggctcatc cgcaactggc
     4561 agaagagttt acccgtcgga tgagcggcgg tttgccgaag gactgggaga aaacgactca
     4621 gaaatatatc aatgagttgc aggcgaatcc ggcgaaaatc gctacccgta aggcttcgca
     4681 aaatacgctt aacgcttacg ggccgatgct gcctgagttg ctcggcggtt cggcggatct
     4741 ggctcccagc aacctgacca tctggaaagg ttctgtttcg ctgaaggaag atccggcggg
     4801 caactacatt cactacgggg tgcgtgaatt tggtatgacc gctatcgcca acggcatcgc
     4861 gcaccacggc ggctttgtgc cgtataccgc gacgttcctg atgtttgttg aatacgcccg
     4921 taacgccgcg cggatggcgg cactgatgaa agcgcggcag attatggttt atacccacga
     4981 ctcaattggt ctgggcgaag atggtccgac gcaccaggct gttgagcaac tggctagtct
     5041 gcgcttaacg ccaaatttca gcacctggcg accgtgcgat caggtggaag cggcggttgg
     5101 ctggaagctg gcggttgagc gccacaacgg accgacagca ctgatcctct ccaggcagaa
     5161 tctggcacag gtagaacgaa caccggatca ggttaaagag attgctcgtg gcggttatgt
     5221 gctgaaagac agcggcggta agccagatat tattctgatt gccaccggtt cagagatgga
     5281 aatcaccctg caagcggcag agaaattagc gggagaaggt cgcaatgttc gcgtagtttc
     5341 cctgccctcg accgatattt tcgatgccca ggatgagaaa tatcgggagt cggtactgcc
     5401 ttcaaatgtt tctgcccgcg tggcagtaga agcgggtatt gccgattact ggtacaaata
     5461 tgttggcctg aaaggggcaa ttgtcgggat gacaggttat ggggaatcag ctccggcgga
     5521 taagctgttc ccgttctttg gctttaccgc cgagaatatt gtggcaaaag cgcataaggt
     5581 actgggagtg aaaggtgcct gatggtgatc gccggatgct gattgtcgga tgtgacgctg
     5641 gtgcgtctta tccggtctac atgtccccgc cattttgttt aacgggtgat ccacaacgtg
     5701 ggccaggcgt ctggcccatg ccagttatcg caggtgggtt ctgcggcgta acgcaccagg
     5761 cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc
     5821 ccttttgcta aggtcaccag ttcacgcgat ttttcatcga atgttgcgtt catcagttcc
     5881 agttcattgg tctcctgacc gttgttgaac ggcaaacgca accgtaccgg gcgcgaggcc
     5941 tgcggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca
     6001 cagctaatca tcatcagcgc tttatcatcg gtcagcgcag tgacattcac ctcacgacgt
     6061 aacggatcaa gcgaacagcg cagaccattc atccgccagt ttccataatc cagcaaatcg
     6121 ttacgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc
     6181 gcaggcgctg gcggtacgct gagcggcggt tcgtcccctt tctttatcca cgcggtctca
     6241 ctgccaacgc gcttttgctg ggcatcaata aacaacaacg ccgcttttaa gccactcaga
     6301 gaaatagtct gattgccgtc gcgtaaggtg atcgccctcc cttcctgaat catctgcaaa
     6361 aacgcggtga tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg
     6421 tcaccactta acgccaacgg ctcaccatct aacagcagcc gtggcgctat ctccccttct
     6481 gacgcatccg gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc
     6541 ccggcgctgc ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgctaca
     6601 cagaaatttt gattattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc
     6661 gcccacacca aagacatcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatatcgc
     6721 atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag
     6781 gtagcgcggc tcaatcggat ttgtcggatt gagccaataa taaacaacga aatattattg
     6841 ttatttttca gtccattaaa tgtgacattt gcaaatagtt aagcaataac accgtcttac
     6901 cgtcacgtat ctcgccggtt ttaatcatct ccagcgcctg gctgaacggc agctcaagca
     6961 cttcaatatc ttcatcttcg acaccaccac cggcgttagc gcgctggctg tcactgtatt
     7021 cggcgataaa aaagtggatt agctcagtca caccgcctgg cgacatatac agttcaaata
     7081 atttgcggac ttcgccaact tcatagcccg tctcttcaat cgcttctttg cgaatgcaca
     7141 cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagttgc ccgctttcat
     7201 tgccattaac ccaggtagcg acgcggaact ggcgaatcag aaccacggtc tttttcttcg
     7261 cgttgtacag gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga
     7321 taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat
     7381 cggagagaat tttgtcttta acgagggtga tttgttgcgt cataccgact ccacagcgcg
     7441 aaatgaacaa ttatcttacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat
     7501 ttgactgatt ttacgcccag ccagtcaatg atcccctgtg ccgcatgacg tccttctgcc
     7561 attgcggtaa ccaccagatc cgcaccacgc acggcgtcac caccggcgaa gattttcgga
     7621 ttcgtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct
     7681 accgttacac cgtgcgactc cagccacggc atcccgtgcg gattgaagcc aaacgccata
     7741 atcaccgcat cggctggcat gacaaattca ctgccttcca ccggcactgg acgccgacgt
     7801 ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac gaatcccgca gacgtgacct
     7861 tgttcattca gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttca
     7921 cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg
     7981 caggtgacgt tactcgcgcc atggcgcagc gcggtacgca cacagtccat cgcggtgtcg
     8041 ccgccgccca gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc
     8101 tcttcgagtc ccatcacctg tttagtgttg gcgatgagga acggcagcgc gtcataaacg
     8161 cccggcgcat cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg
     8221 aagaccgcgt cgtattgttc caaaagcgaa ttcaaagaga catctttacc cacttcacaa
     8281 ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat
     8341 ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga
     8401 tcgtacaccg tcaccgccac gccattgcgg gtcagaacat ctgcacaggc cagccctgcc
     8461 ggacccgcac cgataatcgc cacccgcttg tccactttgg tgacatggct taagtcagga
     8521 cgccaacctt tcgccaacgc ctgatctgaa atgtagcgtt caatgttacc gatagttact
     8581 gcgccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg cgggcaaacg
     8641 cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg
     8701 tttccggctt tcaccagttc aatccactgc ggtatatggt tatgcagcgg gcaggtccat
     8761 tcacaaacgc tgtgctcgcc gcacttaagg cagcgcgagg cttcccgttg tgcctgatcg
     8821 gcacggaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta
     8881 tctggctcgc cacgcgcggg cgttgcctgc atttgctcga ctttactcat taccggcatt
     8941 tcttgcgccg cggtactggc atgccacggt tgatgttcct gacgtgcggt gcgcaagcgg
     9001 cgggatttcg ccatgccgga gagtgcggcg tcagtgacca gttgcagcgc gtccgccggg
     9061 caattttcga cacaggcagg accgttttcg cgccctgcac aaaggtcgca tttatgcgcc
     9121 gtggctttta cttttcctgc cgcaacgggt gtcagaacga tttgcatcgt accaaatgga
     9181 caggccacca cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca
     9241 tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg
     9301 tgacaggtca cggcactacg ttgctgctga tgtttgataa ccgtaattcg gggatgaaaa
     9361 tggtgttggc tcaggacatg ttgctcgtca ttgtgagcca tgacacaggc gatttcacaa
     9421 gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc
     9481 ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg
     9541 gaatcggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat
     9601 caaataatcc catcactgac taaattgcgt ttcagcgaac tggaagatta atgatttttt
     9661 gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt
     9721 ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac gctggcgagc
     9781 agtttacgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc
     9841 ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca gttatttcag
     9901 caggcactgc attcaccggt attaaccaat ctcaacgtct ggtatgtgcc agaggcagta
     9961 aaaacccgct atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag
    10021 ggcgacttgc cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc
    10081 gtgctggctt tacagcacta cgctgaacgc aaaatgctgc tggtggtggc aatttccctg
    10141 gctggcggca tcggtatttt cacgctggtc ttttttactt tgcgccgcat acgccatcag
    10201 gtggttgccc cgctgaatca gctggttacc gccagtcagc gtattgaaca cgggcagttc
    10261 gactcgccgc cgctggatac cagcctgccg aatgagcttg gcctgcttgc aaaaaccttt
    10321 aaccagatgt cgagcgagct gcataaattg taccgttcgc tggaggcgtc agtagaagaa
    10381 aagacccgcg atctccacga ggccaagcgt cgtctggagg tgttgtatca gtgttcgcag
    10441 gcgctaaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc
    10501 gacaatgaag cggctgaata tctggagtta aatgtcggtg acaactggcg gattagcgaa
    10561 gggcagccaa acccggaatt gccgatgcag attttaccgg tgacaatgca agagacggtt
    10621 tacggagaac tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagc
    10681 gtttcgtcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag
    10741 ttattgttga tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc gctggctcag
    10801 gtactttctt acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac
    10861 gccaccgcac aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag
    10921 ttacgcgagc tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg
    10981 ttgagggaaa tgctggatac gttacaaaat caaaccagcg ccaaactgac cctcgactgc
    11041 cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca aattattcgc
    11101 gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt tagctgcgtc
    11161 accgcgccgg atggcaatca cacagtctat attcgcgaca acgggattgg catcggtgaa
    11221 ccgaaagaac ccgaaggcca ttatggtctg aatatcatgc gcgaacgcgc ggaaagattg
    11281 ggtgggacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt
    11341 cgctctgcgg agggtgagga aagtcagctg atgtaatgcc tcttattgac caaagaatag
    11401 tggcacttaa ggttcagtat aaaagggcat gataatttac attaactcct ttttttctcc
    11461 acgattggct cgtaccttgc cgctacagtg aagcaagcca agcctacaac gatacgcaga
    11521 aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt
    11581 gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc ccgttgaaca
    11641 ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc
    11701 ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa
    11761 tctgatgtat atgtcatctc agagcagcgg caccggtcag gcatctgtca cgttaagttt
    11821 taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc
    11881 catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga
    11941 taccaacatt ctgaccattg ccttcgtctc taccgatggt tcgatggata aacaggatat
    12001 tgccgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga
    12061 tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa
    12121 cagtttccag atgacggcta aagatgtcac cgatgccatt gaatcacaga acgcgcagat
    12181 tgcggttggg caacttggtg gtacgccttc cgtcgataag caggcgctca acgccaccat
    12241 taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa
    12301 tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa
    12361 atacgattat cttagccgct tcaatggtaa gccagcctcc gggctggggg taaaactggc
    12421 ctccggcgcg aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgagctggc
    12481 gcagtatttt ccacacggac tggaatacaa ggtggcgtat gaaaccacct cttttgtaaa
    12541 agcctccatt gaagacgtgg tgaaaacgct tctggaagct atcgccctgg ttttcctcgt
    12601 tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt
    12661 ggtgttgatg ggaaccttct cggtgcttta tgccttcggc tatagcgtca acaccttaac
    12721 catgttcgcg atggtgctgg cgatcggtct gctggtggat gatgccatcg tggtggtgga
    12781 aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc
    12841 gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg cggtatttgt
    12901 gccaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat
    12961 tgttgcggcg atggtgctgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc
    13021 cacactactt aagccactga aaaaaggtga gcatcacggg caaaaaggct tttttgcctg
    13081 gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct
    13141 ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct
    13201 gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cgtggcatgt ttactacctc
    13261 ggtacagttg cccagcggtt caacccagca acagaccctg aaagtcgttg agcaaatcga
    13321 gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ccgtgggttc
    13381 tggccctggg gggaacgggc aaaacgtggc gcgaatgttt atccgcctga aagactggag
    13441 cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt
    13501 taacaagatt aaagaagctc gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg
    13561 tagttctgcg ggttttgata tggagttgca ggaccacgct ggagcgggtc acgatgcgct
    13621 gatggcagca cgaaaccagt tgctggcgct ggcagcggaa aacccggagc taacccgtgt
    13681 gcgccataac ggcctcgacg acagtccgca gttgcagatt gatatcgacc agcgtaaagc
    13741 tcaggcgctg ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg cctggggttc
    13801 gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc
    13861 tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa ataaagatgg
    13921 cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct
    13981 ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag
    14041 taccggtacg gcgatggata ttatggaatc gttagtgaag cagttgccaa acggctttgg
    14101 tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct
    14161 gtacgccatt tccttgctgg tggtattcct gtgtctggct gcgctgtatg aaagctggtc
    14221 ggtgccgttc tcggtaatgc tggtcgtgcc gctgggggtt atcggcgcgc tgctggcaac
    14281 ctggatgcgc gggctggaaa acgatgttta cttccaggtg ggcctgttaa cggtcattgg
    14341 tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg
    14401 ccacgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat
    14461 gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacag gtgccggttc
    14521 cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcgg ccactattct
    14581 ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc
    14641 gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcccttt tcaactttcc
    14701 gattaagaac ctgctcagcg ggttcttgct gtttgtactt tgtctcagga attacttacg
    14761 aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc
    14821 tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat
    14881 tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat
    14941 gatttgta
//
LOCUS       DACADA010000114        14518 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.116, whole genome shotgun sequence.
ACCESSION   DACADA010000114 DACADA010000000
VERSION     DACADA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.116"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..98)
                     /locus_tag="ISB17_003390"
     CDS             complement(<1..98)
                     /locus_tag="ISB17_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP1981289.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
     gene            244..423
                     /locus_tag="ISB17_003391"
     CDS             244..423
                     /locus_tag="ISB17_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1981290.1"
                     /translation="MRPSKRTVRFETQPGYHLSATPGKKLIWWLPDNRALGLTAASLN
                     HAEYQRRALPVKRET"
     gene            1623..2768
                     /locus_tag="ISB17_003392"
     CDS             1623..2768
                     /locus_tag="ISB17_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981291.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTKWLKKEPRASYF
                     FLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPHPIPEMD
                     SSIVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAM
                     GFPSQPKIAGPREKPMRTVHPDNIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDEPKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(3299..3556)
                     /locus_tag="ISB17_003393"
     CDS             complement(3299..3556)
                     /locus_tag="ISB17_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981292.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(3610..4377)
                     /locus_tag="ISB17_003394"
     CDS             complement(3610..4377)
                     /locus_tag="ISB17_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1981293.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPELLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(4374..5432)
                     /locus_tag="ISB17_003395"
     CDS             complement(4374..5432)
                     /locus_tag="ISB17_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1981294.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(5451..6440)
                     /locus_tag="ISB17_003396"
     CDS             complement(5451..6440)
                     /locus_tag="ISB17_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1981295.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(6451..8616)
                     /locus_tag="ISB17_003397"
     CDS             complement(6451..8616)
                     /locus_tag="ISB17_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1981296.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVHDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSICHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            9045..9479
                     /locus_tag="ISB17_003398"
     CDS             9045..9479
                     /locus_tag="ISB17_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981297.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            9697..12081
                     /locus_tag="ISB17_003399"
     CDS             9697..12081
                     /locus_tag="ISB17_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP1981298.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNEKRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDECQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSVGRLFVLVNKFDEKDRNGDGADAVCQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFLDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            12078..12983
                     /locus_tag="ISB17_003400"
     CDS             12078..12983
                     /locus_tag="ISB17_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAP1981299.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAG"
     gene            12980..14050
                     /locus_tag="ISB17_003401"
     CDS             12980..14050
                     /locus_tag="ISB17_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP1981300.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPVTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            14186..>14518
                     /locus_tag="ISB17_003402"
     CDS             14186..>14518
                     /locus_tag="ISB17_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981301.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTT"
BASE COUNT         3685 a         3736 c         3797 g         3300 t
ORIGIN      
        1 gtattgcggg agatccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc
       61 aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag
      121 cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt
      181 gacacaatgg gccatacggg ccgacgttcc atgccgcgtt actacattca gcccggacgt
      241 aaaatgcgcc cgtcaaaaag aacggttcgc ttcgaaactc agccaggata ccacctctcc
      301 gccacacccg ggaagaagtt gatatggtgg ttgccggaca acagagctct tggattgact
      361 gctgcatcac tcaaccacgc tgaatatcaa aggagagcgt taccggttaa aagagaaacg
      421 taaagctgtg gtgatgaccc aaaacgcaac gcctgtcagt gaggatgaaa tggtgaaaag
      481 cggacagcat cagtaacgga agtatcttag cgggtacgaa aatggcagga tcgtgacatt
      541 gacagaatat ctcctgatca tcctgaatga ctgcagttgc tgtggaaaag taagctgtct
      601 ccacccacgg cacgacttaa caggcattcc gtaactcccc cagacttact atgacgcatt
      661 atcggtgcac ttaaaaccgc agaccgcatt gcgggtgtta ctgcatgaca gtctgaccgt
      721 attgtgagat gtcggcagga accgatgtcc gcgcaggggg gaacttcctg cagtacctga
      781 cgcttcaaat agtattaacc gtagggcgtt gttggttccg gatcgcggaa atgggagcac
      841 aggtctgcta cggggtgtac ctctgacagt aaatccggct cgctcatctg acagcctctc
      901 tcccggaaaa gaccaaaaat gagatcgtct cctcaacagc agagaagagg tcttgacaga
      961 caggctacat tgcatacgat ttatgaagaa aaagacagca aatatggagg ttatagcgga
     1021 gaacacccta cgaaattcct atgaaaaacg attgaaaaaa atatcaaatt cgattcgttt
     1081 ttatatgctt tttgggaacg tggcgatgac gaactgttgc atgtgcagag gagaatgcac
     1141 ccggaagcac cagaatcatc cagaaaggca gaacgattac gcatcaaacc aggagacagc
     1201 aatatcaagg taatacactg atttaaaatg aatttacagc aatacgtgaa aacatttttt
     1261 acttaaagga gacagatgcg ctgaagagag tgcaggtatt tttatataaa aatgagaaga
     1321 ttacgggata tatatccggc acactatgac cggagaatac cagtcaggct taaaaagaca
     1381 ataaatacag tgagttaaat acacagagaa aacgaaaacg tagtatcaga gggtacatct
     1441 tacaacagag actgaatttc cggtttttca taaccggcgc attccggaaa atgcaggcgg
     1501 cccggagctc ttctgacgcc agtacctgat agattgttct gtacattctg tacatcatgt
     1561 acggaggttt ttgtttgctt tcttaaaatc tgcaaatgtg ggaagagcag gtgaggtgta
     1621 ttgtgggaaa ggaactggat gatatctgca ccagctgtcc gtatatcgat gctgtaaaac
     1681 ggcacaaaca acagctcggt gccatagagg aatatacaaa gtggttaaaa aaagaaccac
     1741 gggcatctta tttttttctg ttccggctgt atacccgcat tcacaataca tttttccccc
     1801 aaaagcaaca actcccgttc actcccggtg gcactcactg cccggaacct gatgtcaccc
     1861 ttcgggactt aacgctttcc ccgggctacc acagtgatta tgcccctcac cccattccgg
     1921 agatggacag ttctattgtt gttcccccca ctaatgaaaa cacctcgcct ccggaagata
     1981 ctcccgacaa cactccggcc ggaggcaata ccggacaagc ggaaaagacc agaaacgcag
     2041 gactcacacc cattcctgaa aaacgttcag gaatgccccc ggaacatctg cggtttgcca
     2101 tgggttttcc atcgcaaccg aagattgccg gtcccagaga aaagccgatg cgcaccgtgc
     2161 atccggataa tatctaccgt gaaattatat ggttctgctc cagttacctg ctgaaatccg
     2221 gaccagaggc tacgaggacg attatcaact cagtattctc tgaatgggcc agcataaaca
     2281 atgattaccc ctcccccttt tcgtgggtgg acagcaggga cagtgaacag tgtgactggt
     2341 tatggaacgc catgcaggtc cggtgtgtgg gaaccccgct gaatcccctt accccggagc
     2401 agaaatactg gttcgcctgc gccacgtttg ataactggga aggctggaat gagcaacagg
     2461 tacagttttt actggaaagt aatcccagac ggaacagagc gaagtttacg caggcctcct
     2521 tccaggcccc caggattcag cataaagcga ttcttcttga tgagccgaaa agtgcccggg
     2581 agcaacaaaa aaggcgtgat gaacgtgctg atggttccgt cccgctgaaa ctgtccggaa
     2641 aaatccacaa acagcttgaa agtattgccc gaagtcgtgg tgtcctccca aaaaaactgc
     2701 tgaatgaaat gattgagcag gcgtaccagg actttgtggc aaatgagcaa cacaagacac
     2761 tgagttaagg accgcagggc ctccggttac cgattctgca gcatacacaa cgaatgtgtc
     2821 cggctcttac ctgtgggggc cgcactaaag ttacgcattg agtatgagtg ggtaatcccg
     2881 gatgagaata tcccagtttt tacactattc actgaaggtg cgacatatca ttcagtctct
     2941 ctgaattctt catttcagtg gccccggcta cagtcgttta atctcctcca cgaaccgggg
     3001 ccgcaacttg ttgtgtcaat accaaattgt tggtcattac tgtcttgtgt cactggcgct
     3061 gacaagtgaa ctgaaatccg caccttaata ttctgactgg ctgcgtacat tcatggcatg
     3121 aagcggccgg agcggctgaa ctgaaggtcc gttgtgagcg aacagcggac attcactaca
     3181 catggttcaa acatgaaccg gagctttatt gtctagctgt cagtgtactg cgatttaccc
     3241 gctcggctgt ccagaaaatc ggtctgaaat ccagtcatta aagggcctta gcctgacatt
     3301 aagcaacttg gtaggcggat agataataga aataggaatg cgcaggtctg tcatgttgcc
     3361 tagaacggtg acaagctgtc cggtgcgaaa tcattccggc agcgacaggc tcatgtgtca
     3421 gcccgacgcc cagaccgtca acgagcatgt ggatctgcga ggcgttatcg ttcgcagcga
     3481 tgttaaaacg aacaatctgc tcatcttcta gaagcacaag tgctttaatg acattaaggc
     3541 ggtctatatg ttgcattaca gacttcctat tgatctttac ctgaggctga agcaaccacg
     3601 ttaatccgac tatttttcga ttgcaccatc cactatcacc atcgatcttc cctgagcaca
     3661 acgttcaact ctcccgcgta cgccgtatac ctgcgcaagg ctctctgcat ggatcaccgt
     3721 ttgcgggtcg ccgctgtcga taagtttccc ctctttcagc atgatgacct gggcggcatg
     3781 ccgcagtgcg atattgatat cgtgtaagac gaccagcgta accatattgc gtcttcgcgt
     3841 ttcgcgcgat acaacatcca tcacatggaa ctgataattg agatccagcg cgctcagtgg
     3901 ttcatccagc aataataatt cggggcggcg aataagcgac tgtgcaagcc ccaccagctg
     3961 cttctgaccg ccggacaggc tatcgaggta gttcattgcc agatgtgcga tgcctagctc
     4021 ttcgagcagc gctatagcct gcgcctgatt ttgcccggcg ccggaggcgc gctgggcgac
     4081 aaccaccgac tccagcacct gtaaatgcac gccctgcggc agggactgcg gcaggaacac
     4141 aactttttca gcccgacgag caaacggcag cgataagaga ttctcttcat tcagccaggc
     4201 ttcgccactg gcgcgattta gcccggccag ggcgcgcagt agcgttgatt taccgcagcc
     4261 attaggcccc agcagtacag tgacttcgcc gcgcggcagc gtagaaatcg acagatgctc
     4321 aataatccgc cgtttgccgt aaccggcgca cagcgcatta atcgtaagcc cgctcatgac
     4381 atactccctc gatgacgcat tacaatgctc agaaagaacg gaacgcctac cagcgaggta
     4441 acaattccta ccggtaaaat gacaccggga atgatgttct tcgacgctac cgaggccagc
     4501 gatagcacca gcccaccgat aaggatgctt ccgggcaaat aaaagcgatg atcttctccc
     4561 agcaacaggc gggaaatatg cggtgcaacc aggccaataa agccaattgg ccccacaaac
     4621 gcaaccgaaa gggccgccag caggctgatg cgcagcaggg aacccaggcg caaacggcga
     4681 acatcaatgc caaaactcat cgcccgctct tcgccgaggc gtagcgcggt aagctgccag
     4741 gcacgacgca gcgaccaggg taaaacaatc gccatcgccg ccgccagcac cgccagtttc
     4801 tcccatgatg cgcgagccag gctgcccatc gtccagaaca ccaggccctg caaagtatct
     4861 tcatcggcga caaactgcat aatggacacc agcgcattga aggtaaacac cagcgcaatc
     4921 ccaaacagca caacgcccga cgtcggcacg cgcgtccagc gggtcagact atcgagcagc
     4981 agggcggaca ggagcgcaaa aagaaaagcg tttgccggta taaacccgct ttcgggaacg
     5041 ccgggaatac cgatgcccag aacaatcgcc agcgctgcgc caaaggccgc cgccgacgaa
     5101 acgccaagcg taaacgggct ggcgagggag ttattcagaa tagtttgcat ctctgccccg
     5161 gccaaaccca gcgccattcc taccaggagc gccatcagcg catagggcaa acggatgtcc
     5221 caaacgataa cccgcatgcc gttagtcgcc ccggacggat gcatcaaagt ctttatcagc
     5281 gaatgtaaag ggagccctga ggggccgagg gtgaaatcga gcagcagcga agcaatgata
     5341 gcgataaaaa taaccgccag tattaccaga cgctggcgaa aaagctggcg atagcggttc
     5401 tcgaccgaca gcgtactttc caatacagtc atggtttttc acagcctccg ttagcgcggc
     5461 ggattgagca catcgctcat ctgcgcatcg cttaacgtca ggccaaagaa ctgccgataa
     5521 aactcacgcg tctctttgcg tatatccaca tcagcaaatc gcgcaggata aagcgttttc
     5581 gccagccaca aaaattgcag cgcctcttcg ctggtttcac ggcaccacca gaacattcct
     5641 ttcggattaa cgtaaacgcg atgatgaatg accgcgtcca cgcttgccca ctgcggagaa
     5701 ctcagaatgg catccgcgtc gcgcttgttc atcgcgatga taaccgccgg attggcagtg
     5761 acaattttct ccagcgcgac ttcacccgaa cggttttgtt gatgctcgcc aaaccaattt
     5821 tcagcgatat ttttgccgcc ggccaggtca atccagtcct ggtttagcga gggtctaccg
     5881 gtggtggtca gcggattgcc catactgtga tagagcgaca cgcgctgtga cgcaggaaga
     5941 tctttcaggc gcccggtgac cagggccacg ttgcgatcga agtagcgttg ataggcgagc
     6001 gctttttgct gcgcatcggg ccccagcacc gctgccgttt gctgtacgcg ctgcgttaac
     6061 gcccgcagtg aattcgcctc aaacgccagc acctgcaccc cagcctgttt cagcaacgcc
     6121 tgttgaggca ctaccatatt ctgcggtaca aaaagcatat cgacccccag cgcgataatc
     6181 tgttcgggat tgatttcatg cccgctgttt acgctaactg ctgcgacttt ctctatgcgc
     6241 ggcaggctct ggcgaaacag ggggatcctt tttgcaatca tcgaggttcc gacaatgtta
     6301 tcgccgtacc ccagcatggc aataatggta ttttgcgccg gccacggtga cgcgatacgc
     6361 acctccttgt gcgtattttc tgcgtgcagg ctggccgcca gcagcagcgc cagcagcgga
     6421 aataaacggg agagtttcat catttatcca tcagaaatcg aaggtgacgg atgcacgcac
     6481 ttcgcggccg ccgccgataa acgcacttgg ggaaccgtta tcgccatcgg tacccgatgg
     6541 gaagatagaa gcccagtaat gtttatcaaa cacgttgttt accaccacgc ggaaagtggt
     6601 tgggacgttg ctcactttcg tggtgtaacg cgttcccaaa tcccatgtgg tatagctgct
     6661 ggcgtaagag gtgttggtat cgttcgccgc gcgtttgccc gtataatgga cgttagcgct
     6721 gtaaacccat tccggtatgg acggcaaact gtattccgcc aacagattgg cctgcacttt
     6781 cggcacgccg acaacctgtt tattgctggt tgaggcattc gccgtatctt tcagtttcgg
     6841 gtcgaggaag gtcacgccgc tgtaaatatt cagcccctgc cacacattcc cggcagcggt
     6901 taactcaagg ccgttgttaa cctggttacc ctgctcttta tacacgttat ccgtatcaag
     6961 ataggcaaac ggacgttcca gtcggaacag cgcggcgcct agattcatct cgccgatgtc
     7021 cgattttagc cccacttcat actgcttgct gcgatacggg tttagcgttt gaccggcatt
     7081 ttttacgctc tcatccgtcg gtgcggtacc gccctgctcc agcgaatcgg catagctaac
     7141 gtaggccatt gtgttagggg taattttata catcagcgcg gcgttcggac ttaaaccatt
     7201 ttcatcaacc tggttcgttt gattaccgtg cttatcgtag tttttagtct gcagccagct
     7261 ctggctgaga tagaacattg ccgaccactg cggcgtaaat gtcaccgtat cgccgagcgt
     7321 aacgctctgc tgagtattta cgctggagcg gtatcgcggc ccgccggtgc ggattttgcc
     7381 atcgccctgc tcatctatcg catccgggtg atacatattt gttgtacccc agctatagct
     7441 ggaacctgtt cctttggcac tataaagcga ccagacatat cccgtggtag aaagtgacag
     7501 atcgtggcag atagagccgg tatcaatatg accattcagc ccggcggtgt tgctcaggac
     7561 gcgaaaacgt ccggcagcgg tggaatcctt catcgaacga gagatatcgc cctgattgtt
     7621 gagtatttta ctggaaacac tacgcatcgc gcggtcagcc tgctgccagc ccacgccagc
     7681 gttcatggac cagtcgtcgt taaagtagtg gatcaggcga gtgctgatgg tatcggtagt
     7741 gaggtcgttg cccgcagtgc tgagcgccag atttttgtcc ttcggattcg gcgcagacgg
     7801 cagtttgacg ttcggcccat agttaaagct accgacatag cctttctgga taaattcgta
     7861 atggctggcg tcgagctgta gctgagtgcc cggctgaata ttccagtcga gcgcaacgga
     7921 aacgagtttg cgacgcagcg tgctgtcatg cacattgcct tccccttcct gatcaagcag
     7981 gttcacgcga tagccaaacc gtttgttttc atcaaaatgg ccacccagat cggcatggcc
     8041 ggtaaacgca ctgcggcttt gatagcccag cgtcacttta cgcagcgtct cttcggttgg
     8101 gcgcttcgcc acgaaattaa actgccctgc tgggctcgcc gggccgtaca gcgcgccggt
     8161 caaactgtta agcacatcca tgcgctcaag catttccacc ggaaacgcgg ttgttgaaac
     8221 gatattcagc ccgtccagac ggctgttggc caccacgctg ccctgcatcc cccgactttg
     8281 cggacgcccg acatccattc caccgcgcgc ctgcatttgc gtactgggag agtatttcag
     8341 catctcgctg acgctttgcg cctgctgatt ttcaatcatc tctttagtaa cagtggtgct
     8401 ggaatatggt gtttcgatcc aggatttcgt tcccagcggg ccgacatcta tatcgttcgt
     8461 tttgaaaccg gcaccggcgg cagaatcggt ggtgtcggcg gcggtgttgc ccgtgacaat
     8521 catttcatct tcagaagtag acgaggcatg agcatgaagc ggtatgaaag acgctaatgc
     8581 tggcaaaagc gcagcgaaag ttatttgagt tttcatgaat cactgatcgt tatgtaaata
     8641 tttatattac gatcggtgca taggcaaaat tatgatccag atcacacgtg aatgtaagtt
     8701 atttacaaat tacgctaacc ttctgtctcc gctctttgca gcataaaagt atggcggcgc
     8761 gttgttgaaa gcaggagcgg ccgggttgtc agaaagctgg tttggacgca agtaatatat
     8821 aacaactctg attaccgcat actgtcagga cagtatctac ttgagcgtcc gcctttggca
     8881 caaagcggac tgtcagatta gattcagacc tgtactcatt agctgataca aatcagccac
     8941 atatacctgc attcaaaata atttcagaaa gatattatct ccgtgaatcc cccgccacct
     9001 ttccggtgca cggggttttg tcttttttca ctgggaatac atgtatgaat ccgtctgatg
     9061 ccattgaggc aattgaaaaa ccgctctcct ccctgcctta cccgatttcc cgtcacatcc
     9121 tggaacatct gcgcaaactc acccgtcacg aacccgtgcc tggcattatg ggtaaaagcg
     9181 gggccggtaa atcctcactc tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg
     9241 cactcatgac agtgcttccc ggacatgccg ccatccctct gatgacacgc ctgcaggacg
     9301 aactgcgcac ggagtctgtc cgcactcaga cccgtgaaca gtttaccggt gcggtggacc
     9361 ggatatttga cacggcggag agtgtctgca ttgcctctgt tgcacgcacg gttctgcgcg
     9421 ccgtccgtga ctcggtggtc tctgttgccc gcgcggtatg gaactggatt ttcttctgaa
     9481 cccggtggtg atgtaccagg ctgttgttac agatttttaa ctccatgaaa acactatgca
     9541 aacgcagcca tacccggggt gtccccttgc gttgtgtttt tcaagttaat agaatacatc
     9601 gatcgttact atcatatcga tcgttataaa cgatcgtttg tggtaaatga cctcctccgt
     9661 ggttgtcatg aaaatgcgtc tcaggaaggt taaataatgc acgaaaagaa catcgccctg
     9721 ctttgtgatg aagccgaccg acttttgcaa ctgaacatta atctgctccg gcaaatggtt
     9781 gaggagccag atgtgttatc tgacagtaag aacgaaaaaa gactgctttt tgataaacag
     9841 aaagcactga aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc ccgcagggag
     9901 atggtgctgg ctgttgtcgg cacgatgaaa gcaggtaaat caaccaccat caacgccatt
     9961 gtggggcagg aaattctgcc taaccgtaac cgccccatga cctctgtacc gacgctcatc
    10021 cgccacgttc ccggaaaaac tgagccggtt ctccatctgg aacatattca gcctgtccgc
    10081 aatttattaa tcacactgca ggaaaaactc gccaccccgg caggacagca ggtcgcacag
    10141 accctgcagc aaaccgggga tacccgcgaa ctgctggata ttctgacgga tgatggctgg
    10201 ctcaaaaatg aataccacgg ggaggaggaa atctttaccg gactggcatc gttaaacgat
    10261 ctggttcgtc ttgctgcggc aatggggagt gaatttcctt ttgatgaata cgcagaagtg
    10321 cagaaactgc cggtgatcga cgtggaattc agccatctgg tggggatgga tgaatgccag
    10381 ggaacactca cactgctgga tacccccggc cctaatgaag ccggacaacc gcagatggaa
    10441 gtgatgatgc gggatcaact gcagaaagcc tctgcggtgc tggcggtgat ggactacacc
    10501 cagatgaact cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat tgctgatgtg
    10561 tctgtcggcc gcctgtttgt gctggtcaat aaatttgatg aaaaagaccg taatggcgat
    10621 ggggctgatg cagtctgtca gaaggtaccg gcaatgctga acagcgatgt gctgcccgcc
    10681 tcccgcgttt atcccggatc ctcacgccag gcatacctgg ctaaccgtgc gcttcatgag
    10741 ttacggaaaa acggaaccct tcctgttgat gaagcctggg tcgatgattt tgtcagggaa
    10801 gccttcggtc gcatgaaaaa agattacgtc tgtaaagaca gcgaactggc aactgaaggg
    10861 gcgacagacc tgtgggaagg ctcacttatc gatcaactga taacggaagt catactgagt
    10921 tcacactcca gagctgcggc actggcggtt gattctgccg ccgcaaaact gatgcagaat
    10981 gcagaaaata tcagtgaata cctgtcgtta cgccatcagg ggctgatgca aagtattcag
    11041 tcactgcagg cgcatatcac cagcctgctt gaggatatcc gggaaatagc agactgtcag
    11101 gagcaggtga ccgctgatgt cagaatggcc atggaggaga tcgatgccag aacccgggaa
    11161 ttactgacgg gggtctgcac ctcactggaa gaggagctga atgactattt cagaagcggt
    11221 aaacgcaaag aacagcaaat gctggaggaa gaaaattcag cgcagccccg ggagagaaac
    11281 gcattcgctt tctttctcga tattttcgga accggaaatc agcatgacag gatgcgggac
    11341 tttgatcccg acagccccga aataaaattc agtgatcgca gggaagccct tgaactgatg
    11401 acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca gttccggcct
    11461 gagctggaga aaatcgtcag cgggattgaa acaggttttc gtggcacggc cctgtacgcc
    11521 acagaaaaca ttgccggtcg catcaatacc cgcctggagg atgagggctt taccgtaaaa
    11581 atcagttttc cggcagtcag tcagttacaa acccggctcg cggtaaaaac aaatctgagt
    11641 gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag tggtttatgg
    11701 ggaaagattt gtggagcgtt tggcaccagt gactggggct gggaaaccta taaagaggat
    11761 gtgagccgca gtgttatcaa tatcaacacc gtcaggaagg aagttatgtc actgacccgg
    11821 gcatatttcg gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag
    11881 gaaatcgatg cctttttctg cgcattcagg gagaaagttg aacaactgcg taacacgctg
    11941 attcagagct ctgaagatca taaacgcgat cagcaggcac aggaacggct taccaggaga
    12001 cttcaggcat taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa
    12061 gagctggaga caatgctgtg acttcaccat ttattcaaca aattgctgat aaccgggtat
    12121 gtcaggtact cacctgtctt cctgaaaaat ttgtggttga tttcgcaaac ggaatcgatg
    12181 ttgcacagga gcatatccgc acggccggag agcgcacgtt cttccggcgt ttaaaagagg
    12241 gactgaccgg cgaaggcgct gcacggcaga atgccatcaa tgcttcggtt gcacagggcc
    12301 ttgaggcgtc cctgcgctgg ctgacggagc tgacaacgtc acttgccacc acaaactacg
    12361 cgattacccg ggtaaacgac agggtcagtt cactggtcag tgatactgcc aggctggcgc
    12421 attattccgc agatacacgg gagcagttac tcatcctggc cgatcaggtt caccataaac
    12481 tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc
    12541 ttgagcagat attctcatgg tggagcgccg ggcgatatgc gtcattttcc cctgccggac
    12601 gttgttatgt ggcgcttgaa gagcttcgct ggggcgcgtt tggtgatgtg atacgtcagg
    12661 gcgaaacagg ccaggttaac cagctactgg atattctcag acataaagca ttaacgcaga
    12721 tggcacagga gagtggcggt agtgcaaccg tgcgtctgaa cactctggac tggcttggtg
    12781 gtcagggccg ggagcaggca gataacgagt ggcatgacgc gattaactgg ctgggagact
    12841 ggtgcagtga agagcagcat ccggtgatct ggtcaaccac gcaggctgca gaacacttac
    12901 cggttcgcat gcctcgcctc tgctctgcag aacgcctctc tgaaagtatg gtcgatgaaa
    12961 tatttcagaa gggggctgga tgagtacaga aatgaaaacg gggctggtgc tgtccggtgg
    13021 cggggcggtg ggcgcttatc aggcgggagt ggttaaggca ctggcagagt gtggtacaca
    13081 gatcagcatg gtttcagggg ccagcattgg cgcattcaat ggtgccatta tcgcggcctc
    13141 tcccgatctg tcagaagctg ccgtacgcct ggaggcgctc tgggatcatc tggggaataa
    13201 tcaggtgctg tcggtaaacc gattggttta cttttcattg ctgaaaaaat tgttccaggc
    13261 aatgaacctc tgccagatcc ccggacgtgc aggagcactg cttacgacgc ttcttcgcca
    13321 tatatcgata ctcaacgggt ttgacaatct gatggctcag ccgttgttgt cagatgagcc
    13381 cctgacagcg ctgttggatc attatcttga tactgatgct ctggcagacg ggctaccgct
    13441 gtatgtgtcg ctgtacccca cagaaggggg catgcaggat attattgact gcattcgtgc
    13501 tgaactgggt gccggaacca cgaaaaacgc cgtttttcag catatccaga gcctgccccg
    13561 cggacagcag aaagaggctc tgcttgcgtc agccgcgctg cccctgctgt tccgtccccg
    13621 tgaggttcag gggacaatgt tcggtgatgg tggtatggga ggatggcgaa atatgcaggg
    13681 aaatacccct gtgacgcctc tggtcgatgc cggatgcaat atggtgattg tgacgcatct
    13741 gagtgacggt tctttatggg atcgccaggc ttttccggtc accacaatcc ttgagatccg
    13801 tccccggaaa aggctgaaat atgcaggtga tggtgacaac agcggcggtc tgctcagttt
    13861 cacgttggca cataccgacg cctggcgtca gcagggctat gaagacacga tgctgacgat
    13921 ggagcatatc cggaaaccgc tggcagcacg tcaggtactg tcccggtcag aaacggtgtt
    13981 gcagaaaagt ctggagataa cggaagaggc agatttggca ctgagaaacg cgatggcccg
    14041 gattaaataa agacgcccca aagaaacgcc accgcctgtg gtggtgtttt tcctggctgg
    14101 tgttattgaa aatattttca gatatatatt acctgagaga aagttttacc cctcacctgt
    14161 caattcactc actggaaatt taattatgca attacctgta aagttattaa tgagccttat
    14221 atctctggtc agcgttattg cacgtgccgg gaaatataaa aattacatcc gggatgaaat
    14281 aaaatactgg cgatatacat catacaaggg gggggaattt ccggaaggtt tcactgatga
    14341 gaaattttcc agcgccattt acaacggaag aatatttaca atgaaacgtt tacataccct
    14401 gatgttattt ctggcggttc tgtttactgg ctttaacgtg gaagcagcga gcgtgaaaca
    14461 agcgctcagc tgcgacccaa acgcccgggc tgaacaacct ggagcgtgtc caacaacg
//
LOCUS       DACADA010000115        14414 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.117, whole genome shotgun sequence.
ACCESSION   DACADA010000115 DACADA010000000
VERSION     DACADA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.117"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(51..926)
                     /gene="rluB"
                     /locus_tag="ISB17_003403"
     CDS             complement(51..926)
                     /gene="rluB"
                     /locus_tag="ISB17_003403"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAP1981302.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1139..3034)
                     /locus_tag="ISB17_003404"
     CDS             complement(1139..3034)
                     /locus_tag="ISB17_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAP1981303.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGISEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(3062..3682)
                     /locus_tag="ISB17_003405"
     CDS             complement(3062..3682)
                     /locus_tag="ISB17_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP1981304.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(3679..4560)
                     /gene="yciV"
                     /locus_tag="ISB17_003406"
     CDS             complement(3679..4560)
                     /gene="yciV"
                     /locus_tag="ISB17_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAP1981305.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            4698..4742
                     /gene="trpL"
                     /locus_tag="ISB17_003407"
     CDS             4698..4742
                     /gene="trpL"
                     /locus_tag="ISB17_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAP1981306.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            4834..6396
                     /gene="trpE"
                     /locus_tag="ISB17_003408"
     CDS             4834..6396
                     /gene="trpE"
                     /locus_tag="ISB17_003408"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAP1981307.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            6396..7991
                     /gene="trpD"
                     /locus_tag="ISB17_003409"
     CDS             6396..7991
                     /gene="trpD"
                     /locus_tag="ISB17_003409"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAP1981308.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRCVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            7995..9353
                     /gene="trpCF"
                     /locus_tag="ISB17_003410"
     CDS             7995..9353
                     /gene="trpCF"
                     /locus_tag="ISB17_003410"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAP1981309.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            9365..10558
                     /gene="trpB"
                     /locus_tag="ISB17_003411"
     CDS             9365..10558
                     /gene="trpB"
                     /locus_tag="ISB17_003411"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAP1981310.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            10558..11364
                     /gene="trpA"
                     /locus_tag="ISB17_003412"
     CDS             10558..11364
                     /gene="trpA"
                     /locus_tag="ISB17_003412"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAP1981311.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPVGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKATTRR"
     gene            complement(11415..11555)
                     /locus_tag="ISB17_003413"
                     /pseudo
     CDS             complement(11415..11555)
                     /locus_tag="ISB17_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            11740..12015
                     /locus_tag="ISB17_003414"
     CDS             11740..12015
                     /locus_tag="ISB17_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1981312.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            12012..12569
                     /locus_tag="ISB17_003415"
     CDS             12012..12569
                     /locus_tag="ISB17_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP1981313.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(12981..13250)
                     /locus_tag="ISB17_003416"
     CDS             complement(12981..13250)
                     /locus_tag="ISB17_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1981314.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            13307..13975
                     /locus_tag="ISB17_003417"
     CDS             13307..13975
                     /locus_tag="ISB17_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1981315.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(14030..14128)
                     /locus_tag="ISB17_003418"
                     /pseudo
     CDS             complement(14030..14128)
                     /locus_tag="ISB17_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518755.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(14174..14356)
                     /locus_tag="ISB17_003419"
     CDS             complement(14174..14356)
                     /locus_tag="ISB17_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1981316.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
BASE COUNT         3617 a         3757 c         3535 g         3505 t
ORIGIN      
        1 tcaaatgaag aaaaggcccc gcagggcctt tatttaatac ttaacttaaa ttaaccgtta
       61 ttattacgtc cgccagaacg acgaccgccg ctcacctgac tgtgacgttt caccgcacga
      121 cgaatctgat tcgccttcat acgacgacgg tctttttcta cagcgacttt agagctggtt
      181 tctggcggta gttccaccag ttcgcgcaga tagttagtct gggcgaggtc cagctccgtc
      241 cagccaccgc gtggcagacc ttttggcagt gggatatcac cgtaacgaac gcgaatcagg
      301 cggctcacct gcacaccaac cgcttcccac agacgacgaa cttcacggtt acggccttcg
      361 gtcagagtaa cgttgtacca ctggttgatc ccttcgccgc cgctgaactt gatggtttta
      421 aaggctgccg gaccatcttc cagctgcacg ccacgactca aatcacgcag tttcgcgtcg
      481 tcaacctgac caaatacacg cacggcatat tcacgttcaa cttcacggct tggatgcatt
      541 aaacggtttg ccagttcacc gtcggtggtg aacaacaaca gaccacaggt gttaacgtcc
      601 agacgaccta cggcaatcca gcgtgcgcca cgcagctttg gcagacggtc aaacaccgtt
      661 ggacgccctt ccggatcgtt acgggtacac aactcacctt ccggtttgta ataggccaga
      721 acgcgacaaa tttgttcagc ggactcacgt accgaaatca ggtgaccatc gatacggatt
      781 ttcagtccag gggtaatttc aacgcgatcg ccgagtttag caattttgcc atcaacactc
      841 acacgaccgg cttcaataat agattcgatt tcacggcgag aaccgtggcc ggcacgcgcc
      901 agcactttct gtagcttttc gctcatagag cttccttttg gtgtcgcctt cccaggcgtc
      961 tgatatgctt ttgtgcaaca atgagtcaga acttacatct gactcactga tagtaaagtc
     1021 tgtttgaact cttcaggcct taagtaaacg actcgacgtt ttaactggcc tggctttgag
     1081 gcgcaacata ctacactatc tttatggtta aagcttgttt cgattaacat cagctttatt
     1141 accagccacc accgcctccg ccacccgcac cgccgccagc accaccaccg gaataacttc
     1201 gccccaacaa actggtattg gcccccagac tcagatgcat ctgtaatgag aacaaaacgc
     1261 cactacgggc aatttccggg gcgcggacag ccgctgtgag tgatggggtg atacgtgcag
     1321 cccattctct gccaaggttt gccgagaccg cccaacctaa caaagcgtaa tccagtttcc
     1381 gtgcttcccc tttcctccgc cgcccactgt gtgttgctgc ctctttacgc gcaagatagt
     1441 taacgatagc ttcaccgtgg gcataacgtt gctgcccagt ttgggtaaac tttggcagaa
     1501 aaatataccc catgccagtg agataaccgg tcaggaaaac agcgctaaaa taacccgctg
     1561 gcatatacca gtaaaagaat acatctccga cattgatgta gagagaatag aaggccacgc
     1621 ctgaacaaat cagtggtaaa aacaaagtga tgattacacg attaacgcaa ggcttgtcat
     1681 catcgcgggc aaacaaaagc tcaagggaac aaaataagaa aggaagaagc cagcagatca
     1741 taataaacat atcgcctacc agactcattc ctgcccaacc tacgggactc aggttaccgt
     1801 agagaatgac cgccaatatc gccatcccgc cccattgcaa aaacgcatca ggtcggtaaa
     1861 acgaagactt tttacgcggc agataaaatt tctgcatccg taaaaatgcc ttgcgtaaga
     1921 ctctgttgcg tctccccgta agaacgacct tatcgccttt acgaaataac gcttccagta
     1981 ataactgctc tgcgggcagt aataccgcat tatttctggt cgcgccttca ttaacacgaa
     2041 tcaggatttg ctggttcttg tcataatgag cttccagatg aatttttcct ttcacaatca
     2101 gatttacgat atcggcgcaa aaacctttat cgtcataaac cagcttcgca tcgagacgta
     2161 acatgccggg cgtgtaatct ggcggaatga catcggtttc aatcacatct accggcgtaa
     2221 attgcggcct gcgcttccag agatataacc atccacacgc caggaacagg cacggaaacc
     2281 aaattaacaa cgatgacgta gagggtaaaa gaagatggct gaaaatattc gtcgtttgtg
     2341 gtgccggggc attaccaagt aaagcgtgag gccagcggta gaggaccgtg aaatcttcac
     2401 gataaaacgg atcgcgagat tcaattcttc cgtcctcaag gatgcggcca tttcgatatg
     2461 tgtcgccctc atctccggta aagagatcga tttcgctaaa tggattaccc gcaatatcag
     2521 gcaacttgag tgaaaatagg gtcttatata tttcaaacgg ccagtggtta ccggtcacgt
     2581 tccagattag cagatcagaa tccccctcac gcaggaaagc atttttaacc tgataatgaa
     2641 tttgataatg atagtcgcct tgaggcaaga aacgttgttt atcgcccacg acaatactca
     2701 ttaacccgat atgccagtca agatgccagg gttcagaaat accatcacgg gtgacgccaa
     2761 caatatgata gttcagcagt gcagcgtccc catcaggctg catccagagt cgcggtaaat
     2821 cacgataaaa tccgtgctta atctgctgat ttcgcgcctg aaaatgaatg ttctctttga
     2881 tgtcgagcac cccattcggt tgcagagtgg cttcaacatc aaagagcgag atcccttctt
     2941 cataagaagg gatctccgtg tttttcgcat aactcaacga agagaaaaaa agcccaacta
     3001 tcaatagcaa aatgcagcga tatatccctg ccataatctt ccatccgtgg aacgcaattt
     3061 gttataagaa aggcttcaca tcacctacgc cttcacgcac cacgaccggc gtatcatcgg
     3121 tgagatcaat aaccgttgtt ggtttctgcc cgagatagcc gccgtgaata atcaaatcca
     3181 cctgtttttc cagacggtct ttaatttctt ccggatcgga ttcggtaaat tcactgcctg
     3241 gcagcattag cgaagtggaa agcatcggtt cgcccaatgc ttcaagcagc gcctgcgcaa
     3301 tcgggttaga cggcacgcgc ataccgatgg ttttgcgttt ttcctgcaac agacggcgtg
     3361 gcacctcctt agtccccttc aggatgaagg tatagttgcc tggcgtgttg tttttcatca
     3421 aacgaaacgc aacgttatcg acaaatgaat aggtcgagag ttcagaaaga tcgcgacaca
     3481 tcagggtaaa gttgtgaccg tccggcagct ggcgaatacg acaaatacgc tccatcgcgt
     3541 ttttatcttc aattttacag ccgagcgcat agccggaatc agttggataa acaatcaccc
     3601 cgcctttacg cacgatctcc accgcctggt tgatcaggcg ttgctgcggg ttatcaggat
     3661 gaatataaaa aaactggctc ataattccct ctctgtggtg ttctgcggct gttcccatag
     3721 ctgccagacg ccttcaacgc ctgcgggcag ccagagttta cgacccagtt cgatccacgg
     3781 gcatggttga tgaaaatcag atccttgtga tgcccataaa tgatgctgac gcgcaagtgt
     3841 cgccagctgg gtgcgttcat tgggcgattg ctggcactgc gcgacttcca tcgcgtcgcc
     3901 gtggtgttcg gcaaaatgcg caaccaatct tttcagccat ttagcactaa gattgtaccg
     3961 ccctggatga gctagcaccg ccttaccgcc agaatgatga atgacatcaa tagcttgttc
     4021 tattgtacac cactgtggcg gaacgtatcc ggttttcccg cgcgccagat actttttaaa
     4081 gacatccgcc attgaactgg cttttccgca ctccacaagg aaacgcgcaa aatgaccgcg
     4141 cgtcaccgca ccgccctgcg ccagttgttg cgcgccttcc agtgccccag gtatttgtgc
     4201 tttttcaagt ctttcggcaa tcagctgcgc ccgctgattg cgccgttctg tctgctgcgc
     4261 gaggaactca cacattagcg gatgagtaat atcaatattc agcccgacaa tatgaatttc
     4321 atgattttcc cagaccgtgg aaatttccac accaggaata agattcagcg ccagtcccga
     4381 acgtgaaatt tcctctcttg ctggtgcaat tgcagctgtg gtgtcatggt cggtgatcgc
     4441 cagggtgccg acgcgcatct cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
     4501 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gagtaattcg tgtcgctcaa
     4561 ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata
     4621 ttctgaaatg agctgttgac aattaatcat cgaactagtt aactagtacg caagttcacg
     4681 taaaaagggt atcgacaatg aaagcaattt tcgtactgaa aggttggtgg cgcacttcct
     4741 gaaacgggca gtgtattcac catgcgtaaa gcaatcagat acccagcccg cctaatgagc
     4801 gggctttttt ttgaacaaaa ttagagaata acaatgcaaa cacaaaaacc gactctcgaa
     4861 ctgctaacct gcgtaggcgc ttatcgcgac aacccaaccg cgctttttca ccagttgtgt
     4921 ggggatcgtc cggcaacgct gctgctggaa tccgcagata tcgacagcaa agatgattta
     4981 aaaagcctgc tactggtaga cagtgcgctg cgcattacag cattaggtga caccgtcaca
     5041 atccaggcac tttccggcaa tggcgaagcc ctgctggcac tactggataa cgccctgcct
     5101 gcgggtgtgg aaaatgaaca atcaccaaac tgccgcgtac tgcgcttccc gcctgtcagt
     5161 ccactgctgg atgaagacgc ccgcttatgc tccctttcgg tttttgacgc attccgctta
     5221 ttacagaatc tgttgaatgt accgaaggaa gaacgagaag caatgttctt cggcggcctg
     5281 ttctcttatg accttgtggc gggatttgaa gatttaccgc aactgtcagc ggaaaataac
     5341 tgccctgatt tctgttttta tctcgctgaa acgctgatgg tgattgacca tcagaaaaaa
     5401 agcacccgta ttcaggccag cctgtttgct ccgaatgaag aagaaaaaca acgtctcact
     5461 gctcgcctta acgaactacg tcagcaactg accgaagccg cgccgccgct gccggtggtt
     5521 tccgtgccgc atatgcgttg tgaatgtaac cagagcgatg aagagttcgg tggcgtagtg
     5581 cgtttgttgc aaaaagcgat tcgcgccgga gaaattttcc aggtggtgcc atctcgccgt
     5641 ttctctctgc cctgcccgtc accgctggcg gcctattacg tgctgaaaaa gagtaatccc
     5701 agcccgtaca tgttttttat gcaggataat gatttcaccc tgtttggcgc gtcgccggaa
     5761 agttcgctca agtatgacgc caccagccgc cagattgaga tttacccgat tgccggaaca
     5821 cgtccacgcg gtcgtcgtgc cgatggttca ctggatagag acctcgacag ccgcatcgaa
     5881 ctggagatgc gtaccgatca taaagagctt tctgaacatc tgatgctggt ggatctcgcc
     5941 cgtaatgacc tggcgcgtat ttgcaccccc ggcagccgct acgtcgccga tctcaccaaa
     6001 gttgaccgtt actcttacgt gatgcacctg gtctcccgcg ttgttggtga gctgcgccac
     6061 gatctcgacg ccctgcacgc ttaccgcgcc tgtatgaata tggggacgtt aagcggtgcg
     6121 ccgaaagtac gcgctatgca gttaattgcc gaagcagaag gtcgtcgccg cggcagctac
     6181 ggcggcgcgg taggttattt taccgcgcat ggcgatctcg acacctgcat tgtgatccgc
     6241 tcggcgctgg tagagaacgg agtcgccacc gtgcaagccg gtgctggcgt ggtacttgat
     6301 tctgttccgc agtcggaagc cgacgaaacc cgtaataaag cccgcgctgt actgcgcgct
     6361 attgccaccg cgcatcatgc acaggagacg ttctaatggc tgacattctg ctgctcgata
     6421 atatcgactc ttttacttac aacctggcag atcagttgcg cagcaatggt cataacgtgg
     6481 tgatttaccg caaccatatt ccggcgcaga ccttaattga acgcctggcg acgatgagca
     6541 atccggtgct gatgctttct cctggccccg gtgtgccgag cgaagccggt tgtatgccgg
     6601 aactcctcac ccgcttgcgt ggcaagctgc caattattgg catttgcctc ggacatcagg
     6661 cgattgtcga agcttacggg ggctatgtcg gtcaggcggg cgaaattctt cacggtaaag
     6721 cgtcgagcat tgaacatgac ggtcaggcga tgtttgccgg attaacaaac ccgctgccag
     6781 tggcgcgtta tcactcgctg gttggcagta atattccggc cggtttaacc atcaacgccc
     6841 attttaatgg catggtgatg gcggtgcgcc acgatgcgga tcgcgtttgt ggattccagt
     6901 tccatccgga atccattctt actacccagg gcgctcgcct gctggaacaa acgctggcct
     6961 gggcgcagca gaaactagag caaaccaaca cgctgcaacc gattctggaa aaactgtatc
     7021 aggcacagac gcttagccaa caagaaagcc accagctgtt ttcagcggtg gtacgtggcg
     7081 agctgaagcc ggaacaactg gcggcggcgc tggtgagcat gaaaattcgc ggtgagcacc
     7141 cgaacgagat cgccggggca gcaaccgcgc tactggaaaa cgccgcgccg ttcccgcgcc
     7201 cggattatct gtttgccgat atcgtcggta ctggcggtga cggcagcaac agcatcaata
     7261 tttctaccgc cagtgcgttt gtcgccgcgg cctgcgggct gaaagtggcg aaacacggca
     7321 accgttgcgt ctccagtaaa tccggctcgt cggatctgct ggcggcgttc ggtattaatc
     7381 ttgatatgaa cgccgataaa tcgcgccagg cgctggatga attaggcgtc tgtttcctct
     7441 ttgcaccgaa atatcacacc ggattccgcc atgcaatgcc ggttcgccag caactgaaaa
     7501 cccgcacact gttcaatgtg ctggggccat taattaaccc ggcgcatcct ccgctggcgt
     7561 taattggtgt ctacagcccg gagctggtgc tgccgattgc cgaaaccttg cgcgtgctgg
     7621 ggtatcaacg cgcggcagtg gtgcacagcg gcgggatgga tgaagtttca ttacacgcgc
     7681 cgacaatcgt tgccgaactg catgacggcg aaattaagag ctatcaattg accgctgaag
     7741 attttggcct gacaccctac caccaggagc aactggcagg cggaacaccg gaagaaaacc
     7801 gtgacatttt aacacgcttg ttacaaggta aaggcgacgc cgctcacgaa gcagccgtcg
     7861 cggcgaatgt cgccatgtta atgcgcctgc atggccatga agatctgcaa gccaatgcgc
     7921 aaaccgttct tgaggtactg cgcagtggtt ccgcttacga cagagtcacc gcactggcgg
     7981 cacgagggta aataatgcaa accgttttag cgaaaatcgt cgcagacaag gcgatttggg
     8041 tagaagcccg caaacagcag caaccgctgg ccagttttca gaatgagatt cagccgagca
     8101 cgcgacattt ttatgatgcg ctacagggtg cacgcacggc gtttattctg gagtgcaaaa
     8161 aagcgtcgcc gtcaaaaggc gtgatccgtg atgatttcga tccggcacgc attgctgcca
     8221 tttataaaca ttacgcttcg gcaatttcag tgctgactga tgagaaatat tttcagggga
     8281 gctttgattt cctccccatc gtcagccaaa tcgccccgca gccgatttta tgtaaagact
     8341 tcattatcga tccttaccag atctatctgg cgcgctatta ccaggccgat gcctgcttat
     8401 taatgctttc agtactggat gacgaacaat atcgccagct tgcagccgtc gcccacagtc
     8461 tggagatggg tgtgctgacc gaagtcagta atgaagagga actggagcgc gccattgcat
     8521 tgggggcaaa ggtcgttggc atcaacaacc gcgatctgcg cgatttgtcg attgatctca
     8581 accgtacccg cgagcttgca ccgaaactgg ggcacaacgt gacggtaatc agcgaatccg
     8641 gcatcaatac ttacgctcag gtgcgcgagt taagccactt cgctaacggc tttctgattg
     8701 gttcggcgtt gatggcccat gacgatttga acgccgctgt gcgtcgggtg ttgctgggtg
     8761 agaataaagt atgtggcctg acacgtgggc aagatgctaa agcagcttat gacgcgggcg
     8821 cgatttacgg tgggttgatt tttgttgcga catcaccgcg ttgcgtcaac gttgaacagg
     8881 cgcaggaagt gatggctgcg gcaccgttgc agtatgttgg cgtgttccgc aatcacgata
     8941 ttgccgatgt ggtggacaaa gctaaggtgt tatcgctggc ggcagtgcaa ctgcatggta
     9001 atgaagatca gctgtatatc gacactctgc gtgaggctct gccagcacac gtcgccatct
     9061 ggaaggcttt aagtgtcggt gaaactcttc ccgcgcgcga ttttcagcac atcgataaat
     9121 atgtattcga caacggtcag ggcgggagcg gacaacgttt cgactggtca ctattaaatg
     9181 gtcaatcgct tggcaacgtt ctgctggcgg ggggcttagg cgcagataac tgcgtggaag
     9241 cggcacaaac cggctgcgcc gggcttgatt ttaattctgc tgtagagtcg caaccgggta
     9301 tcaaagacgc acgtcttttg gcctcggttt tccagacgct gcgcgcatat taaggaaagg
     9361 aacaatgaca acattactta acccctattt tggtgagttt ggcggcatgt acgtgccaca
     9421 aatcctgatg cccgctctgc gccagctgga agaagctttt gtcagcgcgc aaaaagatcc
     9481 tgaatttcag gctcagttca acgacctgct gaaaaactat gccgggcgtc caaccgcgct
     9541 gaccaaatgc cagaacatta cagccgggac gaacaccacg ctgtatctga agcgcgaaga
     9601 tttgctgcac ggcggcgcgc ataaaactaa ccaggtactc ggtcaggctt tactggcgaa
     9661 gcggatgggt aaaactgaaa ttattgccga aaccggtgcc ggtcagcatg gcgtggcgtc
     9721 ggcccttgcc agcgccctgc tcggcctgaa atgccgtatt tatatgggtg ccaaagacgt
     9781 tgaacgccag tcgcctaacg ttttccggat gcgcttaatg ggtgcggaag tgatcccggt
     9841 gcatagcggt tccgcgaccc tgaaagatgc ctgtaatgag gcgctacgcg actggtccgg
     9901 cagttatgaa accgcgcact atatgctggg taccgcagct ggcccgcatc cttacccgac
     9961 cattgtgcgt gagtttcagc ggatgattgg cgaagaaacg aaagcgcaga ttctggaaag
    10021 agaaggtcgc ctgccggatg ccgttatcgc ctgtgttggc ggtggttcga atgccatcgg
    10081 tatgtttgca gatttcatca acgaaaccga cgtcggcctg attggtgtgg agcctggcgg
    10141 tcacggtatc gaaactggcg agcacggcgc accgttaaaa catggtcgcg tgggcatcta
    10201 tttcggtatg aaagcgccga tgatgcaaac cgaagacggg caaattgaag agtcttactc
    10261 catttctgcc gggctggatt tcccgtccgt cggcccgcaa catgcgtatc tcaacagcac
    10321 tggacgcgct gattacgtgt ctattaccga cgatgaagcc ctggaagcct ttaaaacgct
    10381 ttgcctgcat gaagggatca tcccggcgct ggaatcctcc cacgccctgg cccatgcgct
    10441 gaaaatgatg cgcgaaaatc cggaaaaaga gcagctactg gtggttaacc tttccggtcg
    10501 cggcgataaa gacatcttca ccgttcacga tattttgaaa gcacgagggg aaatctgatg
    10561 gaacgctacg aatctctgtt tgcccagttg aaggagcgca aagaaggcgc attcgttcct
    10621 ttcgtcaccc tcggtgatcc gggcattgag cagtcgctga aaattatcga tacgctaatt
    10681 gaagccggtg ctgacgcgct ggagttaggc atccccttct ccgacccact ggcggatggc
    10741 ccgacgattc aaaacgccac actgcgtgcc tttgcggcgg gtgtgacccc ggcgcagtgc
    10801 tttgagatgc tggcactgat tcgtcaaaaa catccgacca ttcctgttgg cctgctgatg
    10861 tacgccaacc tggtgtttaa caaaggcatt gatgagtttt acgcccagtg cgagaaagtc
    10921 ggcgtcgatt cggtgctggt tgccgatgtg ccagttgaag agtccgcgcc cttccgccag
    10981 gccgcgttgc gtcataatgt cgcacctatc tttatttgcc cgccgaatgc cgacgatgat
    11041 ttgctgcgcc agatagcctc ctacggtcgt ggatacacct atttattgtc acgtgcgggc
    11101 gtgaccggcg cagaaaaccg cgccgcgtta cccctcaatc atctggttgc gaagctgaaa
    11161 gagtacaacg ctgcacctcc attgcaggga tttggtattt ccgcccctga tcaggtaaaa
    11221 gcagcgattg atgcaggagc tgcgggcgcg atttctggtt cggccatcgt taaaatcatc
    11281 gagcaacata ttaatgagcc agagaaaatg ctggcggcac tgaaagcttt tgtacaaccg
    11341 atgaaagcca ctacacgacg ttaatttgag acaaaccgcc agaaaacatc tggcggttta
    11401 ttgatggtaa aaaattagaa gcggtaacct gcagagaaca taaataccca cgggtcaagg
    11461 cgtacgttgt cagaataacg ccctgcaggc agcactgcgc cgttggattt atatttcact
    11521 tcggtatcga tatccatgta ccagaccgac atgttttaaa aaatgcaata agacattgta
    11581 tctgaaagaa aaaacaggat ttgaggcgct tttttatacc gtttttccgt gtttttaact
    11641 gcctgatttt attgaaatct tttttagttt tgcagaaagg attttttgct cgatcgcttt
    11701 aattttatac attcctgata acggaaattt ttttgcccgt tgacagtttt tgttctccgg
    11761 ggctatattc ctcacacaac cgcaaaatcg gttgtcggga ttggcgtctc ggataacatc
    11821 acaacgcata gccgcgtttg cggttttttt tgcgttaagc attgccacat tcgcattatg
    11881 gtggggcgtg caggggcgga gaaatccgcg ccgggttttg tgatgaccgg ttacgccaac
    11941 cctgtacgtc tcaccacctc cctgattggc gtcagaggtg gtgatcatcc aattaattgg
    12001 agtcatcaca tatgaataca gctataactt tttctaacga gtcaccaaaa gtctttttca
    12061 aagatggtcg cctggttacc acctctcaag ctgttgccga ctacttcgat aaacaacata
    12121 aacacgtttt agctaagatt gacacactcg actgctctcc cgaatttaca tcagccaact
    12181 tttcggccga tgttcagact gtcgaaattg gcaacggcgc agaacgtgaa tcacgatgct
    12241 acctgatcac aaaagacggc ttcatgttcc tcgtaatggg atttactggc aaaaaagcag
    12301 ccagactaaa agaagcttac attgaaaaat ttaacgcaat ggaagaagaa ctccacaaac
    12361 gtcctcctgc agcacaaaac tcccccgccc caaataatgg atgcgcatta ctgatccact
    12421 tcgataaaca cggtcaggtc gagttcacag aaaaagtccc cgccgatgcg atggtatgca
    12481 cgctggaacg gtttaaattt tatctggagc aacatgggtg gatcgttgcc cgtaaagaac
    12541 aattggtgga aaggttgatg cggttttaat gaatgcaaaa ccccggatga tcaccggggt
    12601 taattttatc gagcgtgatt gccgctaatc gccatcgctc tgattgagcg taaatcattc
    12661 aggttattca gttcttcctt ctgctctcgc tgacgtcaat aaatcccatc attgcgatca
    12721 acctcatcct taaccattga tgcgagcagt tcttccagtt tctgcgctga cagctttacc
    12781 tggtgatctt cagcatcttc ccgggctatt gtggttcgcg cagtggctga ttttgctgac
    12841 atcaccacag ggggcagacg gaccattgaa ccatggcctc cggaacaaat aagggcaaaa
    12901 ataacaacca ctatcgaaag ctcacaaact aaccgcagca cgttcctgca tacgacgtgt
    12961 ctgcggcata atcccaatga ttactccctg acaggatttg caggccactc aatatcaggt
    13021 gcagttgatg tatcaacacg attcaacaat acccgatatt tattccatgc ctccagcaac
    13081 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
    13141 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
    13201 tgtatcgaag cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt
    13261 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaatcaat
    13321 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
    13381 ctggtgggtt ttgggagtat caaatcggct tgcatgggga tgtcctacaa aggaacacct
    13441 tcttccacac tttctggaac atgtaggtaa caaccatctg gatattggtg ttggaactgg
    13501 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
    13561 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca
    13621 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
    13681 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
    13741 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
    13801 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
    13861 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
    13921 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
    13981 aaccgcagca cgttcttgct taagacgtgc tgcggcataa tcccaatgat tactccctga
    14041 cagggttcgt aggccactca atatcaggtg cagttgatgt atcaacacgg ttcagtaaca
    14101 cccgatactt tttctaggct tccaccacca gcacgacaag atgccgcata cagtgaacca
    14161 gtcagtccag ttttcagaca accagtgcgt cacctttttg aaggcgcttt aaagcacgtt
    14221 ttaatccagg tcggcctgtc cttgttccgc ttaatttatc ttcaaatatt tgttcatatc
    14281 ctgcacaaac aagagcgttt cgttgcagat ctgtattctg gtcatttgtt gataccctta
    14341 catagccaat cagcacgctg aatctcccgt ccaaaagcac aaatcatgcc atgcaggcca
    14401 gaaaccgcca ttat
//
LOCUS       DACADA010000116        14373 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.118, whole genome shotgun sequence.
ACCESSION   DACADA010000116 DACADA010000000
VERSION     DACADA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.118"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            164..1198
                     /locus_tag="ISB17_003420"
     CDS             164..1198
                     /locus_tag="ISB17_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1981317.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            1271..2686
                     /locus_tag="ISB17_003421"
     CDS             1271..2686
                     /locus_tag="ISB17_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAP1981318.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            2739..3818
                     /gene="alr"
                     /locus_tag="ISB17_003422"
     CDS             2739..3818
                     /gene="alr"
                     /locus_tag="ISB17_003422"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP1981319.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            3841..4398
                     /locus_tag="ISB17_003423"
     CDS             3841..4398
                     /locus_tag="ISB17_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP1981320.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            4395..5399
                     /locus_tag="ISB17_003424"
     CDS             4395..5399
                     /locus_tag="ISB17_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1981321.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            5481..6674
                     /gene="tyrB"
                     /locus_tag="ISB17_003425"
     CDS             5481..6674
                     /gene="tyrB"
                     /locus_tag="ISB17_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAP1981322.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            6856..9600
                     /locus_tag="ISB17_003426"
     CDS             6856..9600
                     /locus_tag="ISB17_003426"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP1981323.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            9633..10787
                     /gene="odhB"
                     /locus_tag="ISB17_003427"
     CDS             9633..10787
                     /gene="odhB"
                     /locus_tag="ISB17_003427"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP1981324.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            10799..12217
                     /gene="lpdA"
                     /locus_tag="ISB17_003428"
     CDS             10799..12217
                     /gene="lpdA"
                     /locus_tag="ISB17_003428"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP1981325.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLVVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            12239..13408
                     /gene="sucC"
                     /locus_tag="ISB17_003429"
     CDS             12239..13408
                     /gene="sucC"
                     /locus_tag="ISB17_003429"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP1981326.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            13421..14293
                     /gene="sucD"
                     /locus_tag="ISB17_003430"
     CDS             13421..14293
                     /gene="sucD"
                     /locus_tag="ISB17_003430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP1981327.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
BASE COUNT         3409 a         3492 c         3991 g         3481 t
ORIGIN      
        1 atgcgtaatt tgtacgatta gggtgtaaaa aacacaacaa gggaagtgcg aatctgtgat
       61 cgaaacgacg atctcctgcg atcccttgcg ttaacggatc gcatgagtaa aaggagatta
      121 tgataaagta attgccttga tcttatgggg gtttggagca ggcatggcga agtcggatac
      181 aggaagaaag cgggtaacat taaccgatgt tgcgcgtgcg gctggcgttt cgaaatcgac
      241 tgtatcgctg gttctcaacg acagttcact tatcaaaaaa gagacgcaac aaaaagtcca
      301 gcaagccatt gaacaattag gctatgtcta taaccgtttt gctgccaatc tgcgttcgca
      361 aaaatcgcta actatcggcg tagtcattga cgatttgata aatcctttct tcgctgaatt
      421 tactatgggt cttgagatga cgctggcgga gcacggtttt atcaccgtga tgtccaatac
      481 ctcccagcgt agtgaccggc aaaaacaggt gcttgatacc ttgctggaac accacgtcgc
      541 gggcattgtc ctctgtccag tgaacagtac ctcagaagcc gatttacagc gttatgccaa
      601 cagttccaca cccttattaa tcacgatgcg ccctctggat tggcaacaat tgcccgtcga
      661 ttacgttggc gtggacagcc acgcaggtgt acgggaagcg acggaatatc tcattcaaca
      721 agggcacaga gatatcgtgt ttattggtgg tcttacgcac catatgcgtt atcagggata
      781 ccttgaggcg atgaatcacc atggtttaca gccgtggagt actgatgctt tttcgctgcg
      841 tgctgaaccg actcaggcca acggctacca gttgatgcaa cagctgttgg atatgccttc
      901 gccacccaca gcggtgattt gttataacga tttaatggct tttggcgcgg agtcggcact
      961 aggcgaaaga ggattatttg ccggggaaga tatttcgttg attggtaatg atggcgtggc
     1021 ggcttgtgct tacagtaacc cgccgttaac cacaattgcg gtagaaccat tggctctggg
     1081 gaaacaggcg gcgcagcaaa ttttacggcg tattgcgcaa ccagacgcac cgctcagcca
     1141 ttacatctat cgcccgaccc tgcaaattcg cgcttccacc ggaaagcggg ggcgttaaat
     1201 gctgtagcga gaaactgttt ctcgctatac tctcttgtcc cccattcaac agcaggtaac
     1261 accattcgtt atggcaggca atagaccttt caacaaacaa cagactgata accgcgaacg
     1321 cgatccacaa gttgccgggc tgaaagtgcc tccgcactcg atcgaagcgg agcagtcggt
     1381 gttgggcggt ttaatgctgg ataacgaacg ctgggatgat gtagccgagc gtgtggtggc
     1441 agacgatttt tacacccgcc cacaccgtca tatctttact gaaatggcgc gtttgcagga
     1501 aagcggtagt cctatcgatc tgattaccct tgcggaatcg ctggaacgcc aggggcaact
     1561 cgatagcgtc ggtggttttg cttatctggc agagctgtca aaaaatacgc caagtgcggc
     1621 gaacatcagt gcctatgcgg atatcgtgcg cgaacgtgcc gttgtccgtg agatgatctc
     1681 cgttgcgaat gagattgccg aagccggttt tgatccacaa gggcgtacca gtgaagatct
     1741 gctggacctt gctgaatccc gcgtcttcaa aattgccgaa agtcgtgcaa acaaagacga
     1801 agggccgaag aacatcgccg atgtgctcga cgcaaccgtg gcgcgtattg agcagttgtt
     1861 tcagcagcca cacgatggcg ttaccggggt aaacaccggt tatgacgatc tcaacaaaaa
     1921 aaccgctggc ttgcagccgt cggatttgat catcgttgcc gcgcgtccgt cgatgggtaa
     1981 aacgacattt gcgatgaacc tcgtcgaaaa cgcggcgatg ttgcaggata aaccggtact
     2041 tatcttctcg ctggagatgc catcagaaca gattatgatg cgttctctgg cgtcgctgtc
     2101 gcgtgttgac cagactaaaa tccgtaccgg gcagctcgat gatgaagatt gggcacgcat
     2161 ttccggcacc atgggtattt tgctcgaaaa acgcaatatc tatatcgatg attcctccgg
     2221 cctgacgcca acggaagtgc gttcccgcgc acgccgtatt gcccgtgaac acggcggcat
     2281 cgggcttatc atgatcgact acctgcaact gatgcgcgta ccggcgcttt ccgataaccg
     2341 tacgctggaa attgcagaaa tctcccgctc gctgaaagca ctggcgaaag aactgaacgt
     2401 gccggtggtg gcgctgtccc agttgaaccg ttctctggaa caacgtgccg acaaacgccc
     2461 ggtcaactcc gacctgcgtg aatctggctc tatcgagcag gatgcggact tgatcatgtt
     2521 tatctatcgt gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg cggaaattat
     2581 tatcggtaaa caacgtaacg gcccaatcgg gacggtacgc ctgaccttta acggtcaatg
     2641 gtcgcgcttc gacaactatg cggggccgca gtacgacgac gaataataat tattttatga
     2701 attaggtaat taaagcaaac acttatcaag gaacacaaat gcaagcggca actgttgtga
     2761 ttaaccgccg cgctctgcga cacaacctgc aacgtcttcg tgaactggca cctgccagta
     2821 aaatggttgc ggtggtgaaa gcgaacgctt atggtcacgg tcttcttgag accgcgcgaa
     2881 cgctccccga tgctgacgcc tttggcgtag cccgtctcga agaagctctg cgactgcgtg
     2941 cggggggaat caccaaacct gtacttttac tcgaaggctt ttttgatgcc agagatctac
     3001 cgacgatttc tgcgcaacat tttcataccg ccgtgcataa cgaagaacag cttgctgcgc
     3061 tggaagaggc taacctggac gagccggtta ccgtctggat gaaactcgat accggtatgc
     3121 accgtctggg cgtaaggccg gaacaggctg aggcgtttta tcatcgtctg acccagtgca
     3181 aaaacgttcg tcagccggtg aatatcgtca gccattttgc gcgcgcggat gaaccaaaat
     3241 gtggcgcaac cgagaagcaa ctcgctatct ttaatacctt ttgcgaaggc aagcctggtc
     3301 aacgttccat tgccgcgtcg ggtggcattt tactctggcc acagtcgcat tttgactggg
     3361 tgcgcccggg catcattctt tacggcgtat ctccgctgga agatcgctcc accggtgccg
     3421 attttggctg tcagccagtg atgtcgctaa cctccagcct gattgccgtg cgtgagcata
     3481 aagccggaga gcctgtcggt tatggtggaa cctgggtaag cgaacgtgat actcgtcttg
     3541 gcgtagtcgc gatgggctat ggcgatggtt atccgcgcgc cgcgccgtcc ggtacgccag
     3601 tgctggtgaa cggtcgcgaa gtgccgattg tcgggcgcgt ggcgatggat atgatctgcg
     3661 tagacttagg tccacaggcg caggataaag ccggggcccc ggtcatttta tggggcgaag
     3721 gtttgcccgt agaacgtatc gctgaaatga cgaaggtaag tgcttacgaa cttattacgc
     3781 gcctgacttc aagagtcgcg atgaaatacg tggattaata ttcgtctcca gggagggaca
     3841 atgtcttatt cagaaatcgc cgcgtgtctg gcatacgcgg tttccgtctg gctggctgcc
     3901 agaaacaatg tgcatacatg gtggatcggc ataattggca gcatattgta cggctgggtt
     3961 ttttggtccg tgcaactcta tgccgacgtt acgctccagt tattcttcat cgtgaccagc
     4021 atcactggct ggatccactg gctgaaaggt cagggtggcg acatcttgcc ggtgcgccga
     4081 acgcaagcca gtcacttttt ccttttgttg ctctgtgctg tcgtcgtggc aggcggttac
     4141 ggctttttac tccacacctt taccaatgct tgggcaccct ggctggattc gttgattttg
     4201 accttcagcg ttctggcaca attcatgttg atgggaagac gtatcgaaaa ctggtacgtc
     4261 tggttagcgg tgaataccct ggcggtgcca ctgtatatga cgcgcggttt aaacctgacc
     4321 gctggcttat atttcctgtt ctggattaac gcctggtatg gtttgtatca atggcgcaaa
     4381 gagttgcaaa catcatgaag ccttttgcaa cgggtctggt ggttggtaaa ttcgcgcctt
     4441 tgcattgtgg tcatgaaaag ttgatcaata ccgcactggc gcaatgtgaa gaactgttca
     4501 tcatcagtta ttccgtgccg gaaatgcctg actgtgaacc tgaaaagcgc ctgacgtggc
     4561 tgcaagtgcg cttcccgcaa gcgacgtttt tggtactgac gccagagctt gttgcgcgtt
     4621 ataacttacc tgctatcccg cataacaatg ctgatgccga catccaccgt cattatgtgg
     4681 cgacgctatg cttgcagata ttacgttgcc gcccacacgc tgtatttact gcagaagact
     4741 atggcgatgg ctttgctaac gtgctggcgc ggcgttttgc ccaacccgtt gaacacgttc
     4801 gaatggcgcg cccagttggc gatgaagcgc cttcgggaac gcttattcgt tccgatgttc
     4861 accgctatcg ctacatgttg gcgaatgatg tttattacag tttcgttcgc cgtatctgtc
     4921 tgcttggtgg cgaatctacc ggtaaaagca cgctttcaaa agcgctggcg ggcgtgcttg
     4981 atacggtgta tgtcgctgaa tttggccgcg actattggga agaaaaaaac ggtatcttaa
     5041 ctgcggatga tttgttgcat atcgcctgcg aacaggtacg ccgggaacaa caagcagaag
     5101 ctaatcacta tcttatatgt gacacgtcgc cactcacgac cttattctat gcccttgatc
     5161 aatatggtca tgctccgcaa gaacttcacc aactggcgga gcgggaatac tctctggtag
     5221 tgctttgcgg cgcagaattt cccttcgtgc aggatggcac ccggcaagga gaggtatttc
     5281 gcgcacggca acaggtgtgg tatgaacagg aattgtcgcg caggaacatt ccttatcttt
     5341 cggtttcggg gccactgcaa gagcgagtgg agcaaattct tcgccaactt cccgactgac
     5401 tttgaacatc ctctcgatct tcgccttctt ccggtttatt gtggtttaac cacctgcccg
     5461 taaacctgga gaaccatcgc gtgtttcaaa aagttgacgc ctacgctggc gacccgattc
     5521 ttacgcttat ggagcgtttt aaagaagacc ctcgcagcga caaagtgaat ttaagtatcg
     5581 gtctgtacta caacgaagac ggaattattc cacaactgaa agccgtggcg gatgcggaag
     5641 cgcgcctgaa tgcgcagcct catggtgctt cgctttattt accgatggaa gggcttaaca
     5701 gctatcgcca tgccattgcg ccgctgctgt ttggtgcgga ccatccggta ctgcaacaac
     5761 agcgcgtagc aaccattcaa acccttggcg gctcaggggc attgaaagtg ggcgcagatt
     5821 tcctgaaacg ctacttcccg gaatcaagcg tctgggtcag cgatcctacc tgggaaaacc
     5881 acgtagcaat attcgccggg gctggattcg aagtaagtac ttacccctgg tatgacgaag
     5941 cgactaacgg cgtgcgcttt aatgacctgt tggcgacgct gaaaacatta cctgcccgca
     6001 gtattgtgtt gctgcatcca tgttgccaca acccaacggg tgccgatctc actaatgagc
     6061 agtgggatgc ggtgattgaa attctcaaag cccgtgagct tatcccattt cttgatattg
     6121 cctatcaagg atttggtgcc ggtatggaag aggatgccta cgccattcgc gccattgcca
     6181 gcgctggatt acccgctctg gtgagcaatt cgttctcgaa aattttctcc ctttacggcg
     6241 agcgcgtcgg cggactttct gttctgtgtg aagatgccga agccgcaggc cgcgtactgg
     6301 ggcaattgaa agcaactgtt cgccgcaact actccagccc gccgaatttt ggtgcgcagg
     6361 tggtggctgc ggtgctgaat gacgaggcat tgaaagccag ctggctggtg gaagtagaag
     6421 agatgcgaac tcgcattctg gcaatgcgtc aggaactggt gaaggtattg agcacagaga
     6481 tgccagaacg caatttcgat tatctgctta atcagcgcgg catgttcagt tataccggtt
     6541 taagtgccgc tcaggttgac caactacgtg aagaatttgg tgtctatctc atcgccagcg
     6601 gtcgcatgtg tgtcgccggg cttaacacgc aaaatgtgca tcgcgtggca aaggcgtttg
     6661 ctgcggtgat gtgattttaa tcaacaggcg gatctctccg cctgttcctg actccagcca
     6721 ctccctacta ttttgtccct tttttcgccc acttttacat catctctttt ctataagttt
     6781 ttgaaaatta gtgtttttaa ctgttggtac ggattgtgca gtaatgcagc tgtctatgac
     6841 catcaggaga aaaacatgga gaacatgacc tcgcccggca cgctgcttag tggagataac
     6901 gcgacctggc ttgaagagta ctaccagacc tggttgcgta cgccggaaca actcccggaa
     6961 gactggcggc gtttttttct ttcacctgaa ctgactgtcc aaagcgtatc cggtgataac
     7021 aatatcagcg gtgcgacctt aaaaaaacag gctgcggtaa tacaactgat caatgcctgg
     7081 cgcacgcagg ggcatctgcg cgcaaagctt gatccacttg gtttgaatcc cccggctgac
     7141 gttccctccc tccagcctgg tttttgggga ttaagcgaag aagacttgtt gcaggagttt
     7201 agcgtcacct ttggcgcaca caccacgcaa atgccactta agcaactgct taacctgctg
     7261 gagcaagcct gggccagtag ccaggcttat gaacttgctc atctggaaaa tcgtgaagaa
     7321 atcaactggt tgttatcacg gattgagagc agcaatgcgc cacaagctga tgcccaaacc
     7381 tgtattgctc gttttgaaaa actgatggcg gcagagacgt tagagcgtta tctgcatacc
     7441 cgttatgttg ggcagaaacg gttttctctg gaagggggcg aaagtgcaat cccggcgctc
     7501 gatacgctga caaaacgcct gcgtgcgcaa ggtgtggagg aaatggtcat tggcatggcg
     7561 caccggggaa gacttaatgt tctggttaat ctgttgaata aagacccggc acagcttttt
     7621 gctgagtttg aaggcaagca gacgatcggc agtggttcag gcgatgtgaa ataccacatg
     7681 gggtattcca gcaatctgga aacacctgcc ggttcgctgc atgtggcgct ggcgtataac
     7741 ccctcacatc tggagatcgt gaatccggtg gtgctgggcc aggtgcgagc caggcaggaa
     7801 cgccgtggtg aggatggtca ggcaaaagtg gtgggcgtac ttattcatgg ggattccgcg
     7861 ttgggcggcc tcggcgtcaa ccaaaccacg tttaacttat cgcagactca gggctacggc
     7921 actggcggta cgctacatct ggtgattaac aatcagattg gcttcaccac ttcccgcttg
     7981 caggatatgc gttcttcacg gtactgcacc gatattgcca aaatggtagc ggccccaatt
     8041 atccacgtta atggcgatga cgttgacgct gtttgccagg taatggaact ggcgtgcgaa
     8101 tggcgagaca ccttccggcg tgacatcatc atcgatattt gctgcttccg taagcacgga
     8161 cacaacgaaa gcgatgagcc acgtcttacc cagccacaaa tgtatcaggc agtggatgct
     8221 caccctggta cgctcgcccg ttatggtgaa tctcttgccc gccgcggcct gttaactcag
     8281 gcgcaacaag atgagatgac ggcacgctat cgcgactggc tggatagctg ccagaaacgt
     8341 gaaccgcagc ctctcaaacc tgcaattcac tcttttagcg ctaactggta tgggttgacc
     8401 aaccctcact ggagcgcccc ggtatctacg gcattgcctc ggcaaaagct ggcagcgtat
     8461 ggtgaaatca tttctacact gccacccgat gtggtcgcac atcctaccat taaacggcaa
     8521 ctggcgttac gccaggatat ggccgcaggt actcagccag tagactgggg aatggctgaa
     8581 atgctggcct atgcatcgct tgtggatgcg ggtgttggtg tgcgtctttc tggtgaagac
     8641 tcagggcgtg gcacgttcag tcatcgccat gcagtggtac atcatcaaac ggaagcccgt
     8701 cgttatctgc cgttacagca tatccgcgct ggtcaggcat ctttcgatgt ttatgattcg
     8761 gtgcttaatg aagaagcgct cttagccttt gaatatggct attcaacgtc tgcgcctcag
     8821 caactggtta tttgggaagc acagtttggt gactttgcca acggcgctca ggttgctatt
     8881 gaccaattta tctcgtctgg cgaaaccaag tgggatcgct atagcggttt aaccattctt
     8941 ctgccgcatg gctatgacgg acaagggccg gagcactctt ccgcacgtcc tgagcgctgg
     9001 ctgcaactgt gcgcagaaaa caacatgcag gtggtgatgc ccagtgagtc agcacaaatg
     9061 ttccatttgt tgcgcggtca ggcattacgc ccaatgcgca aaccgttagt gataatgatg
     9121 agtaagcgtt tacttcggtt taaaggcgcg atgagcgagc tttgtgaatt cactgatgac
     9181 gcttataaac cggtaatcac cgatccgcaa ttgcatcagt cccagaaagt taagcgcgtt
     9241 attttgtgta gcggccaggt ctattacgac gtactggaag cccgtaagca gcgtgagcat
     9301 gaagacgagg tagcgattgt tcgcctggag caactctacc cgttcccggt cgcagaactg
     9361 aatgatgttc tggcgagttg gccgaactgc tgcgaatgga tttggttgca ggaagagcca
     9421 gaaaaccagg gcgcgtggcg gcagattcgt catgagctgg cggcactgaa aattaacact
     9481 ccttactggc agtatgcagg ccgcccggca gcggcagcac cagcgacagg gtatgggcgt
     9541 gtacataaac aacaaatcga cgaatttctt gctgctgcgt ttgcagatat tcagccatga
     9601 aaggcgcaca aaaataataa ggagaacatt ttatgatcga aattactgtc cctgtattac
     9661 cggaatctgt cacggaaggc acgctgacaa cctggtgtaa acaagaaggc gagcacgtaa
     9721 aacgcgatga tgtgattgcc gagctggaaa ccgataaagt cattctggaa ataccggccc
     9781 cgcacgatgg cgtgttaagc aatattatcg tcagcgaagg tagcacggtc acgtccgcgc
     9841 aactgttggc gcatcttaag ccgcaagctg tcatagaaga aaccgtcaca ccagtcaccg
     9901 aaaccctggc gatgccttcc gcacggctgg aggcacagcg tagtggtgtc gaacttgctg
     9961 acgttgcggg gagcgggaga aatggacgta tcctgaaaga ggatgtgcag cgcgttactc
    10021 ctgcaccagc cattcagcct gaacgggtgg cggagatcgc gccagctaaa cccttgacac
    10081 cgggtgctcg tcaggaacgt cgtgagccga tgtcgcgctt acgtcagcga attgctgagc
    10141 gtctgctggc ttcccaacaa aataacgcca ttcttaccac attcaacgaa gtaaacatgc
    10201 agagcgtgat ggacttacgc gcccgctgga aagatcgttt tgccgagaag cacggcgtga
    10261 agctgggctt tatgtccttc tttgttaagg ccgttacccg ggcgctggag cgcttccctg
    10321 tagtgaatgc cagcgtcgat ggcaacgaga ttatctggcg tgattattgc gatatcggca
    10381 ttgcagtgag cagcaaccgc ggcctggtag tgcctgtgtt acgcaatgcg caatcactct
    10441 ctttggtgga aattgaacgg caaattgccg aatacgccac acaggcacgc aatggcaaat
    10501 tgccgctgga ggcgttacag ggcggcacgt tctctatcac caacggcggt acttttggtt
    10561 cgatgatgtc aacgccaatc attaacccgc cgcagtcggc cattcttggg atgcatgcca
    10621 tcacgccgcg cccggtcgca gaaaacggtc aggttgttat tcgtcccatg atgtaccttg
    10681 ctctcagcta tgaccatcgc attatcgatg gtcaggaagc cgtgcataca ttggtggcaa
    10741 tacgtgagct actggaatca ccggaacaac tgttgctgga tctttaagga gggcgccgat
    10801 gagtacaatt tttgatgtgg cagttatggg gggggggcca ggtggttacg tagcggcact
    10861 gcgggctgcg cagaatgggc tttccgtagt atgtattgat gatggtgtta atgcacaggg
    10921 cgaaccttcg ccgggcggta cgtgtctcaa tgtcggctgt attccgtcaa aatcgttgct
    10981 gcaatcttcc gaactgtacg cgcaagtgca gcatgaggcg agcattcatg gcgttaatgt
    11041 tgacggcgta tctttcaatg cagctgcgat gattcaacgt aaagacgcca tagtaagccg
    11101 cctgacaatg ggcatcagct tgctgtttaa gaaaaataaa gtgaagcacc tgtgcggcct
    11161 cgcaacgtta gaatgtgctc aggatgagat ttggcaatta cgtgtaaacg atcaacacct
    11221 tcacgcccgt aatgtagtga ttgcaacagg ttctcagccg cgtcagctgc ctggcgttac
    11281 cattgataat caacagatcc tcgacaatcg tggcgccctg gcattgagcg aagtgccgcc
    11341 gcgtctcggt gtgatcggcg ctggcgtgat tggccttgaa ctggggtctg tgtggaatcg
    11401 ggttggttca gacgtcacgt tgctggagat ggcgccaacg ttcttgcctg cactggaagc
    11461 tcgcctgtcg aacgaagtcc gtaaagcaat gattgccagt gggatgaaaa tgcaactggc
    11521 agtagagatt gaggctattg aacaacggga tgacggtgtg catgtgcgct ggcggcaggg
    11581 tgagaagcga gaagaaagcc gcttcgataa attaattctt gccattggac gtgtaccgcg
    11641 actttctggt gtcgatctcg tgcaactcgg tcttgaagcc gataaccgtg gcggtattgc
    11701 cgtcgataac ttatgccgta ccgggaaagc cgggctgtgg gcaattggtg atgtcgtgcg
    11761 agggccgatg ctggcgcata aagcgatggc tgaaggtgta gtggtggctg atcaaattgc
    11821 cggactggtg gtagagccga ttaattttgc gctgatccct tcggtgattt acacccaacc
    11881 ggaagttgcg tgggtagggg aaaacgaggc ctcactgaaa gccgctggca gagtctttaa
    11941 caaaggaaat tctctgtttg ccggtaacgg tcgcgcctta gcattaggcc aggaaggtgg
    12001 gcgttgcacg ttatacagtg ataaacacac tgaccgcgta cttggcggag cgattgttgg
    12061 cccgcaggca tcggagctga ttaatgaaat tgcgctggcg atgactttta gcgcctctgg
    12121 cgaggatatt gcctgtgcga tccatgcgca tccgacatta agcgaagtca tccacgaagc
    12181 agcaatggcc ttgaataata aagcattaca cggataacac acaccactga gggaatatat
    12241 gaatttacat gagtatcagg ccaaatcgtt actggctggc atggggatgc cgtgtccaaa
    12301 agagatagct atccaacaga tttcgcaact ggcagatgcc tggcaacata ttgcttgccc
    12361 gagtaaaggc gcggtactta aagcgcaggt acatgctggt gggcgcggta aagctggcgg
    12421 cgtaaaagtg cttaagcagc tgccggaagc acaggctttt gtacagcaaa tgttgggatc
    12481 gcaactggtg acttatcaga ccgggccaga aggacagtat gtcagcagta ttttactgtg
    12541 cgaaaacatt tatccggtac gccaggaact ctattttggc atggtggtgg atcgtgaaag
    12601 ccagcgggtc acgtttattg tcagcccgga aggtggtgtg gaaattgaaa aagtcgccca
    12661 tgagacaccg gagaaaatca gcagtgtcag cattgatcca ctgacaggcg tgcagccttg
    12721 ccatatccgc gaaatgtttg ctgttctgca actggaacac ggactatttg ctgcctttag
    12781 tcgcctggtt aatcaggcgt ggaaagcctt taacgaactg gattttgccc tgctggaaat
    12841 taaccctctg gtgttgcggg aaacgggcga atttatgtgt gcagacgcga aagtatcgct
    12901 ggatgataac gcgctgtatc gtcacccgga actccaggtg ctacgtgatg aaacccagga
    12961 agatccacgc gaaagccagg cagcgaagct tgatcttaat tatgtctcgc tggacggaaa
    13021 tatcggctgc atggtcaatg gtgcagggct ggctatggca accatggaca tcatcaagct
    13081 atacggtgaa caacccgcaa acttcctgga tgttggcggt ggcgcaaccc aggagcgcgt
    13141 cagtgaagcc tttcggctga ttgtttccga cagcaaagtg aaagccattc tggttaatat
    13201 ttttggtggg attgtccgtt gcgacatgat cgccagagcc attattcatg ctctgaatga
    13261 ggccagcatc accctgcctg tcgtggtgcg tctctccggt aataacgccg ccgaagggca
    13321 gcggttactg gcagaaagtg gtctgacggt ggaagccgtc gattctctgg atgatgccgc
    13381 gaaacgcatc attgcgttac tgaattaaca ggaggttaag atgagcgttt tgatcaacaa
    13441 acacacgcgg gtactggtgc agggcattac cggcaaaaac ggcacatttc atacagagca
    13501 ggctattgcg tatggcacgc agattgtggg tggagtgacg cctggtaagg gcgggcaacg
    13561 gcatctggat cgcccggttt ttgattcaat gaaagatgcg atgcgacaaa ctgaggccga
    13621 tgccagcgtg atttatgtgc ctgcaccgtt tgtgctggat tctgttgttg aggctattga
    13681 agctggcgtc aaattggttg tggtgatcac tgaaggtgta ccgacactgg atatggtgaa
    13741 agttcgtcgg ctgcttgact gccacccgga tgtgcggctg attgggccaa actgcccagg
    13801 agttatcacg ccgggtgagt gcaaaatcgg gattatgcct ggtgaaatcc accgcccagg
    13861 gcgtattggg attgtttccc gctccggtac attaacctac gaagctgtag cgcaaaccac
    13921 ggcgctggga cttgggcaat ccacctgtat tggtattggc ggcgatccgg ttccggggac
    13981 taactttatt gatgcgctgc gtttattcga aaatgatccg caaacagacg cggtaattat
    14041 gatcggtgaa attggtggta atgcggaaga acgggccgcg gaatttattc gccatgatat
    14101 gaataaaccg gtcgtaggtt atattgccgg tgttactgca ccaaaaggaa aacgcatggg
    14161 acacgccgga gccattattt ctggcggtag cggcagtgca gaggataaat tccgtgcgtt
    14221 cgataaagca ggcatggcat ggacaagaaa cccggcgttg cttggcgaaa cgttatggaa
    14281 tgttattaag taatccataa atgaatatca agggagcata tcgctccctt tttttatctg
    14341 tgtggtaaga gaatcacctt ttatttactg tgc
//
LOCUS       DACADA010000117        14138 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.119, whole genome shotgun sequence.
ACCESSION   DACADA010000117 DACADA010000000
VERSION     DACADA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.119"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(118..870)
                     /gene="yecC"
                     /locus_tag="ISB17_003431"
     CDS             complement(118..870)
                     /gene="yecC"
                     /locus_tag="ISB17_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAP1981328.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(867..1535)
                     /gene="tcyL"
                     /locus_tag="ISB17_003432"
     CDS             complement(867..1535)
                     /gene="tcyL"
                     /locus_tag="ISB17_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAP1981329.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1550..2536)
                     /gene="dcyD"
                     /locus_tag="ISB17_003433"
     CDS             complement(1550..2536)
                     /gene="dcyD"
                     /locus_tag="ISB17_003433"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAP1981330.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEVAVNISSVVVASGSAGTHAGLAVGLEQLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2641..3441)
                     /gene="tcyJ"
                     /locus_tag="ISB17_003434"
     CDS             complement(2641..3441)
                     /gene="tcyJ"
                     /locus_tag="ISB17_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1981331.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3529..4080)
                     /gene="fliZ"
                     /locus_tag="ISB17_003435"
     CDS             complement(3529..4080)
                     /gene="fliZ"
                     /locus_tag="ISB17_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAP1981332.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4126..4845)
                     /locus_tag="ISB17_003436"
     CDS             complement(4126..4845)
                     /locus_tag="ISB17_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAP1981333.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5009..6058)
                     /locus_tag="ISB17_003437"
     CDS             complement(5009..6058)
                     /locus_tag="ISB17_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAP1981334.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            6306..7721
                     /gene="fliD"
                     /locus_tag="ISB17_003438"
     CDS             6306..7721
                     /gene="fliD"
                     /locus_tag="ISB17_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1981335.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7737..8147
                     /gene="fliS"
                     /locus_tag="ISB17_003439"
     CDS             7737..8147
                     /gene="fliS"
                     /locus_tag="ISB17_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1981336.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8147..8512
                     /gene="fliT"
                     /locus_tag="ISB17_003440"
     CDS             8147..8512
                     /gene="fliT"
                     /locus_tag="ISB17_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAP1981337.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKIKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8590..10077
                     /gene="amyA"
                     /locus_tag="ISB17_003441"
     CDS             8590..10077
                     /gene="amyA"
                     /locus_tag="ISB17_003441"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP1981338.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10111..10524)
                     /locus_tag="ISB17_003442"
     CDS             complement(10111..10524)
                     /locus_tag="ISB17_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1981339.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            10711..11916
                     /gene="yedE"
                     /locus_tag="ISB17_003443"
     CDS             10711..11916
                     /gene="yedE"
                     /locus_tag="ISB17_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAP1981340.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            11913..12146
                     /gene="yedF"
                     /locus_tag="ISB17_003444"
     CDS             11913..12146
                     /gene="yedF"
                     /locus_tag="ISB17_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAP1981341.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12255..12752
                     /locus_tag="ISB17_003445"
     CDS             12255..12752
                     /locus_tag="ISB17_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981342.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(12634..12963)
                     /locus_tag="ISB17_003446"
     CDS             complement(12634..12963)
                     /locus_tag="ISB17_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1981343.1"
                     /translation="MKRLQAFKFRLRPGGQQERGMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            12986..13510
                     /gene="tnpA"
                     /locus_tag="ISB17_003447"
     CDS             12986..13510
                     /gene="tnpA"
                     /locus_tag="ISB17_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP1981344.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(13667..>14138)
                     /locus_tag="ISB17_003448"
     CDS             complement(13667..>14138)
                     /locus_tag="ISB17_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1981345.1"
                     /translation="PQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNC
                     LLLSDSKQSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILD
                     DVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         3494 a         3616 c         3475 g         3553 t
ORIGIN      
        1 aagccatcgc attctcttat gttattaatg agttatgctg atttgttaag cagttttatc
       61 acgtttgaaa tggcgtcccg ccccgactgg tgaatcgtcg gggctgattt tttcttatta
      121 ttgcagcaga aacttctcca ggaactggcg ggtgcgaggc tgctgaggat cagcaaataa
      181 cgcttttgct accccctgct cgactatccg cccctggtcc ataaaaatcg cccggtccgc
      241 aacatcccgg gcaaagctca tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc
      301 cagctgacga atggtgttca ggacttcgcc caccagctct ggatccagcg ccgacgttgg
      361 ctcgtcaaac aaaatcactt caggacgcat tgccagagca cgcgcaatcg caacgcgctg
      421 ctgttgaccg ccagacaaac gacgtggata actggtttct ttaccggcca gcccaacttt
      481 cgccagcagc tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac
      541 cggcccttca ataatgtttt ccagcaccgt acgatgcgga aacagattaa agttctggaa
      601 gacaaacccg acgtgctgac gcaactggcg aatcagcgat ttttgctgac ttaatgaacg
      661 tgcagtatca atagtgatat cgccaacggt gatggttccc gcttccggtt gttccagcag
      721 atttatgctg cgtagcaacg tggttttgcc ggaaccactc ggaccgataa ttgccactac
      781 ttcgcctggc ttcacctcaa ggtcgatacc atgcagcacc gtctgaccgt ggaatttttt
      841 caccaggttc ttaacttcaa tggcactcat ttgggttctc tctcctggcg attaagttga
      901 ttctcaaaat ggttctgcaa cgtcgataac acagtcgcca tgatccagta gatgagcgaa
      961 gccgccaggt acatggtgaa cacctccagc gtgcgcgagg taatcaactg cgcctgacgg
     1021 aacagctccg gcacctggat tgtcgcggcc agcgacgtat cttttaccag gctgataaag
     1081 ctgttcgaca gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcatg
     1141 gtctgccatg gcgtcatacc aatactggct gcagcttccc actgaccttt atcaatagaa
     1201 gaaatggcgg cgcgcagcgt ttcagcagca taggcggcag tgttcagcga cagaccaatc
     1261 attgctgacg gaatgggatc taattcgata ccaaactgtg gcagtccgta atagatcata
     1321 aacagttgtg cgatgagcgg cgtaccacga aaaatagaga tataaaaacg cgccagccag
     1381 cgcacgggcc agattggcga caggcgcatc agcgcgagaa taaacccgag cagtaagcca
     1441 aaaaacatgc ctccgatact gagttgcagc gtatacccag cccctttcag caggaacggc
     1501 aaggaatcaa taaccagttg tatactttct tgcatgagcg ttcccggagc taaacgtggg
     1561 gatgataggc gaacagcgca ggcgcaccgc cggtatgaat aaacaaaatc ggcccttcat
     1621 ctttgaagcg tttctgactg ataccgtcaa tcagccccgc catcgctttt ccggtataca
     1681 caggatcaag cagaatgcct tccagcctcg ccagcaattt cactgcttcc atgccttcgt
     1741 cgttcggcac gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg
     1801 cggtcagctc cagttctttc gcaatcgcct gttggaggtt aaccactttc ggcaattgat
     1861 cggcaactga acgcgacaca gtcacgccaa tcagttcgct ttcaggcatc agctgttcca
     1921 gcccaacagc cagtccggcg tgagttccgg cactgcccga tgcgaccacc accgacgaaa
     1981 tattaaccgc cacttcacac tgttgcgcga tttccagcgc actctccaca taacccagcg
     2041 cgcccaaagc attagaaccg ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga
     2101 ctcgcgtcgc cagctcttcc agttgggcat tgggatcggt cagtgcgtcg cacatttcaa
     2161 tctgggtatt gaacagatcc agcaacaaac gattgccgtt ggttaaatag ttttccgcgg
     2221 ttgtaccaat aggattttcc agcagcgcca cgcagtgcag accgagtttc gcagcgactg
     2281 cggcagtctg gcgcacatgg ttagactgga tcgccccggc agtaatcagc gtatcggcac
     2341 cttcacgcag agcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg
     2401 ccatgggagt gacgtcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc
     2461 gcggcagata ttcgagcggc gttggcgcgc caataaactc cagccgtgga aaacgggtta
     2521 aattatgcag tggcataaca gcctccgatg tgtgttgttg tgattttctt attatgcacg
     2581 ctgaaaacgc gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga
     2641 ttatttggtc acatcagcac caaaccattt ttcggaaagg gcttgcaaag tgccatcttt
     2701 ttgcatttcc gcaattgcat cattcaccgc tttcagcagg tcttcatttc ctttacgcag
     2761 cgccacgcca gactcctgac gagagaatgc ttcaccggtt actgccagcg tatcgttggt
     2821 tttcttcacc agatccagcg ccgccagacg atcaacgagg atcgcatcga tacggcctac
     2881 gcgcagatcc tgatatttgg tcgggtcatc atcataggta cgcacatcga cgccctgaac
     2941 attctgacgc agccactctt catagttggt gcccagaccg acacccactt ttttgccttt
     3001 cagatcatca gccgttttaa tggtgccttc gttgcctttt ttcaccagcg cctgaatacc
     3061 agaaatggtg tacggggtag agaaatcgta ttttttcttg cgctcgtcag aaatggtgac
     3121 ctggttaatc accacatcaa tgcgtttaga gtccagcgac gccagcatac cgtcccattt
     3181 ggtcggtttt agtgacgcct caacgccaag atgttttgcc agttgttggg caaattccac
     3241 ttcaaaaccg gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagttccttc
     3301 cagccctacc agcagcgtgc cgcgctcttt gactttatta agcagacctt catctgcaaa
     3361 acttttaacg ctcatacccg caaccagcgc cacggccatc acacccatca atgcctgacg
     3421 tcccagatgt gctaatttca tattcacccc gaatgttgtt atgtctgttt gcagtgtaga
     3481 gccattgttt gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc
     3541 agaagcaggc aggctggagg atttctgcac aagtccggta cacgtttgac gctgatagtg
     3601 ttgatacttc cgtaacgcga tgcggtaatt gttggtgctg gtggagggca gccacggggc
     3661 gaggatttca tcgaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcgtgtag
     3721 atgattcccc aaacggcgca gacgaacgac atattcacgc accgtaccag ggctcatttc
     3781 agtttgctca aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt
     3841 gcaatgtaaa tcaccgcaaa atcggcacaa tgccgcccat gattgttgtt ccacttgcca
     3901 gccattttca tcaagcagcg tgtccaggcg ggaaatctcg attttattca ctattttgcc
     3961 gtcgcgaacg agggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggtctggct
     4021 gtgtttaaag tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat
     4081 taagaactcc tggtagtcaa aattaaagtg cggcatttac tgacgttata acttacccag
     4141 tttagtgcgt aaccgtttaa tagcctggct gtgtaactga ctgacccgcg attccccgac
     4201 ctccagcacc gcaccaatct ctttgagatt cagctcttcc tgataataga gggttaatac
     4261 cagtttttcg cgctccggca acgtttcgat ggcttccatc acccgctggc gcagattatt
     4321 gtccagcagt tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct
     4381 atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc tggctgttat tggtgtcgag
     4441 caacatttgg cgataatcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt
     4501 ggcgttgcgg ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt
     4561 gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg
     4621 gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac
     4681 ggcattaagt aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg
     4741 cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc gggacataac gctgccacag
     4801 cgagtgttta tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc
     4861 gttatatgag ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt
     4921 gggtgaaatg aggggttatt tgggggttac cggtaaattg cgggcagaaa aaaccccgcc
     4981 gttggcgggg tcaggcaggt taaggccatt agccttgtaa cagagacaga acttgctgcg
     5041 gtacctggtt ggctttagac agcacggagt tgcccgcctg ctggataatc tgcgctttcg
     5101 acatgttgga cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcagaca
     5161 ggttggtggt ggtgttgttc aggttggtga ccgcagaatc cagacggttt tgaacggcac
     5221 cgagggagga gcggaatttg tcgactttag cgattgcggc atccagtgct tttaacggat
     5281 cggctgtagc ggctcccgcc acttccgctg caccaacagt taccgccgcc gccgtaccgg
     5341 taatgactac agtggcttcc atgtatttac cggttgcaga atcaacgacc gcatatttag
     5401 tactgttaga cgcggtgtca gtgtaagaca gtaaattgac ttgtttaccc ccagcatcct
     5461 tgacatccgc agcagcaatt gctgtaccat cagcagccgt cagtttcgcc gcgctgaaat
     5521 ccgccatgcc ggacacggta aatcctttca gattcaaagt ggtactgtcg atttgttgca
     5581 agccaatgct gatcgtctgg ttatcattgg caccaatctg aatcttcatc gaatcgtttt
     5641 tcgacagcac gttcacaccg ttgaactggg tctgaccaga tacacggtca atttcatcaa
     5701 gacgggattt gatttcgtcc tgaatagaag acaggtcgga atcagagttc gtgccggtag
     5761 aggcctgaac ggtcagttca cgaatacgct gcaagttgtt gttaatctct gacagcgcgc
     5821 cttcagccgt ctgcgccaga gaaataccgt cgttggcgtt acgtgcggcc tgagtcagac
     5881 ctttgatgtt agaagtaaag cggttagcaa tcgcctggcc cgcggcgtca tctttagcgc
     5941 tgttaatacg cagaccagaa gagaggcgct cgatagaagt cgacagcgca gactggtttt
     6001 tgttgatgtt gttctgagtg atcagcgaga ggctgttggt attaatgact tgtgccataa
     6061 ttcattttcc tgttttcaag tctacggttt aagtattggg cttccaccct tcggcttcgt
     6121 cgccgtcaac cctgttatcg tctggtcact cacaaccttt agaatttttt tctagcggtc
     6181 gaaatactcc ccttcctcgt tgctgttcgt gccattaagg caaaaaataa atcgtaaact
     6241 ttaccccgct tacgccgata accccggtat tcgttctata cacgtcgcat gattaaggaa
     6301 actatatggc tagtatttca acgcttgggg ttgggtcggg gctggactta agcagcatcc
     6361 ttgacagtct ggaggcggca gaaaaatcta ccctgacgcc gatatcaaaa cagcaaagct
     6421 cttacaccgc gaaactgagc gcctacggga cgctgaaaag tgcactagag agtttccaga
     6481 cagccaacac agcactgaat aaagccgatt tatttaccgc cacctcaacc accagcagtt
     6541 catcggcttt cagcgccacc accacgggca gcgcgattgc tgggaaatac accattagcg
     6601 ttagccaact ggcgcaggca cagacgctga caacaaaaaa tagtcaaaaa gacagtaaag
     6661 ccgccatcgc caccagcgat agcgtactga ccatccaaca aggcggcggt aaagatccgg
     6721 tcacgattga tatcagcgcg ggtaactcgt cactttccgg cattcgcgat gccattaaca
     6781 acgccaaagc tggtgtcagc gccagtatca tcaatgtggg aaacggcgaa tatcgtctgt
     6841 ccatcaccgc taacgatacg ggcagcaata acgctatgaa acttagcgtc agcggcgaca
     6901 gcgccctgga aagttttatg ggttacaacg gaacgccggg tgatagcagc aatggcatga
     6961 ttgaaagcgt caccgcgcaa aacgccaaac tgacagtcaa taacgttgag attgaaaaca
     7021 gcagtaacac tatcagcgac tcactggaag atatcaccct caacctgaat gacgtcacca
     7081 ccggtaatca gacactgacc atcagtaaag acacatccaa agctgaaaat gcggttaaag
     7141 cgtgggtgga tgcctataac acattgcagg atacattcag cagcttaacc aaatacaccg
     7201 ctgtcgatgc cggagctgaa agtcaggact ccagtaatgg cgcattactc ggtgactcca
     7261 cactgcgtac catccagact cagcttaaaa ccttgctcgc caatactcac agcagttcaa
     7321 actataaaac gctggcacag attggcatta ccagcgatgc cagtaccggc aagctggaaa
     7381 tcgcgaccga caaactgcaa acggcgctaa aaaatgatgc cgctggtatc ggtgaaatgt
     7441 ttataggtga cggcaaaagt actggggtaa ccaccggcat tagtagcaac ctgaccagtt
     7501 ggctctcttc caccgggata atccaggcag caaaagatgg cgtcagcaaa acgctgaata
     7561 atttgaccga ccagtacaac gcagccagtg aacgtatcga taccttaatg acgcgctata
     7621 aagcccagtt cacccaatta gatgtgttaa tgaactcgct gaactccact agtagctatc
     7681 tgacacagca gttcgacacc tcaaatagca actcgaaata atcttcagga gagactatgt
     7741 acgcggcaaa aggcacccag gcctatgcac aaattggcgt cgaaagcgcc gtaatgagcg
     7801 ccagccaaca gcagttggtc accatgctat ttgatggagt gttgagcgca ctggttagag
     7861 cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg tcaaaagcga
     7921 tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa gacgaactaa
     7981 cccaaaactt gattgctctt tatagctata tggtcaggcg tttgctgcaa gccaatttac
     8041 gcaacgatgt ctccgcagtc gaagaagtgg aagcattaat gcgcaatatt gccgatgcct
     8101 ggaaagagtc tttactctcc ccttctttga ttcaggaccc agtctgatga acaatgcacc
     8161 gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt tacgcctggc
     8221 aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtga atgcggtgca
     8281 ggagatcgca cacctgactg aagaggttgc cccgtccact acgatgcagg agcagctccg
     8341 cccgatgctg cgcctgattc tcgacaacga aagcaagata aagcagttat tacagattcg
     8401 gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg tgttaagtgc
     8461 ctatggcgat cagggcggct ttgtgctggc tccgcaggat aacctctttt gaatcttaat
     8521 gagccgatgg ctcgcgaata atccgattac ggctacgctt ctaatattcc ccttgaatgg
     8581 agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta cccggaaggc
     8641 ggtaagctct ggcctgaact ggccgagcgc gcagacggtt ttaatgatat tggtatcaat
     8701 atggtctggt tgccgcccgc ctataaaggc gcatcgggcg gctattcggt cggctacgac
     8761 tcctatgatt tatttgatct aggtgagttt gatcagaaag gcagcatccc tactaaatat
     8821 ggtgataaag cacaactgct ggccgccatt gacgccctga aacgtaatga cattgcggtg
     8881 ctgctggacg tggtagtcaa ccacaaaatg ggcgcggatg aaaaagaagc tattcgcgtg
     8941 cagcgtgtaa atgctgatga ccgtacgcaa attgacgaag aaatcattga gtgtgaaggc
     9001 tggacgcgtt acaccttccc cgctcgtgcc gggcaatact cgcagtttat ctgggatttc
     9061 aaatgtttta gcggtatcga ccatatcgaa aaccctgatg aagatggcat ttttaaaatt
     9121 gttaacgact ataccggaga aggctggaac gatcaggttg atgatgaact cggtaatttc
     9181 gattatctga tgggggagaa tatcgatttt cgcaatcatg ccgtgacgga agagattaaa
     9241 tactgggcgc gctgggtgat ggaacaaacg caatgcgacg gctttcgtct tgatgcggtc
     9301 aaacatattc cggcatggtt ttataaagag tggatcgaac acgtacagga agttgcgcca
     9361 aagccgttgt ttattgtggc tgaatactgg tcgcatgaag tcgataaact gcaaacgtat
     9421 attgatcagg tggaaggcaa aaccatgctg tttgatgcgc cgctgcagat gaaattccat
     9481 gaagcatcgc gcatgggtcg cgactacgac atgacgcaga tcttcactgg gacgctggtg
     9541 gaagccgatc cttttcacgc cgtaaccctc gttgccaacc acgacaccca accgttgcaa
     9601 gccctcgaag cccccgtcga accgtggttt aaaccgctgg cgtatgcctt aattttgttg
     9661 cgggaaaatg gcgttccttc ggtgttctat ccggacctct acggtgcaca ttacgaagat
     9721 gtcggtggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca gcttgatgag
     9781 ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cgttattttt cgaccatccg
     9841 aactgcattg cctttagccg cagtggcacc gacgaatatc ccggctgcgt ggtggtcatg
     9901 tcgaacggag atgatggcga aaaaaccatt catctgggag agaattacgg caataaaacc
     9961 tggcgcgatt ttctggggaa ccggcaagag agcgtagtga cagacgaaaa cggcgaagcg
    10021 accttctttt gcaacggcgg cagcgtcagc gtgtgggtta tcgaagaggt gatttaaatc
    10081 catccccggc ggcaagccgg ggagatttca ttacggcagt ggcgtcggta acggcgcttt
    10141 atccagcgcg gcggcacact ctgccgtcgg gcgatccaca cgctccatcg tcataccatc
    10201 gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc tcacgtttta cggtaacgtt
    10261 agtgagatca tcagacatca atgtcaactt acccggcacg gcaatcaccc gctgccactg
    10321 acggcaatcc agggtatcgc cctctttagt gacaatgagg ctgcctatcg cttccggact
    10381 gaccaacgca cgttgcggcc ctttggtctg ccagtaaccc gtcagccagt ccggcgcggg
    10441 ggttttcacg acattgttat agttttcgac ttcggcacag cccgccagca acatgagcgc
    10501 acctgcaatt gcgagttttt tcatcattcg tcctgcatga gaagaaaaga ggattgtggc
    10561 attaaagccc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat ctaaccatta
    10621 tttttgtgaa ttattacccc gaaaataaat ttgcatggtg tatgattcgc gactcttgtt
    10681 tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa acacgcctgg
    10741 ttgattaaat tctgggcgcc catccccgcg gtcatcgcgg cgggtattct ctccacttac
    10801 tattttggca tcactggcac cttttgggca gtcacgggtg aatttacccg ttggggcggt
    10861 cagctcctgc agttgttcgg cgtccatgct gaagagtggg gttattttaa aattatccat
    10921 ctggaaggat cgccattaac ccgcatcgac ggaatgatga tcctcggtat gtttggcggc
    10981 tgttttgccg cagcgctgtg ggccaacaat gtcaaactgc gcatgccgcg cagccgtatc
    11041 cgcattatgc aggccatcat tggcggtatt atcgccggtt ttggcgcacg tctggcaatg
    11101 ggctgtaacc tggcggcgtt ctttaccgga attccacagt tctcgctgca cgcctggttc
    11161 tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct tctgcccatc
    11221 ttccgtattc ccgtgaaaat gcagaaagtt tctgctgcct caccgttgac gcaaaaaccg
    11281 gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat gctgggctgg
    11341 gcgctgctga cggcgatgaa ccagcccaaa ctggggctgg caatgctgtt tggcgtcggc
    11401 tttggtttac tgattgaacg tgcgcaaatc tgctttactt cggcgttccg cgatatgtgg
    11461 atcaccggac gtacccatat ggcgaaagca atcattatcg gtatggcggt aagtgccatc
    11521 gggatcttca gttacgtaca gttaggcgtt gaacccaaaa tcatgtgggc gggaccaaac
    11581 gcggtaattg gtggtttact gtttggtttt ggcatcgtgc tggcaggcgg ctgcgaaacc
    11641 ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg tctgggcaac
    11701 gtgatcggct caacgattct ggcgtactac tgggatgatt tcgctccggc gctggccacc
    11761 gactgggaca aaatcaacct gctgaaaacc tttggcccga tgggcggcct gctggtgaca
    11821 tatttgctgt tgtttgctgc tctaatgttg attattggct gggaaaaacg cttcttccgc
    11881 cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct gattaccgtc
    11941 ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aacccttgag gcgatgccgc
    12001 agttaaaaaa aggggagatc ctggaagtgg tgagcgactg tccgcagtcg atcaataata
    12061 ttccactgga tgcgcgtaat cacggctata cggtgctgga tattcagcaa gacgggccga
    12121 ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtggcccac agcgggaaca
    12181 tcgttacctc tctatcacat agattttacc cgccctctct cctattcttt tcagtgtgct
    12241 ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa gattacctcg
    12301 cgtttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaacct attggcagat
    12361 acaacgtcgc gccgggaacc aaagtccttc tgctcagtga acgtgatgaa caccttcatc
    12421 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
    12481 cccgcgtaga aactgccgca accagtcaca tgtttaaacc gctctggcaa catggtcggg
    12541 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aagcagcctt
    12601 attttatcta tcgtgctgat ggacaagctg tttttatagc cgcgataggt agcacaccgt
    12661 ttgagcgtgg tgacgaagcc ggaagaagtt tttgtaagcc cgctcagggt ctttcagtga
    12721 ctgttgcaat ggctgtgagg gcgaatcttt aagccattgc gtttcagtgg catttttcca
    12781 ctcaaccaac caggaagcca ttttcccgta agggatgtat ttattaccgg cctcatgatt
    12841 ctcattctga agcgccagag cacgattgaa aacgaaacga catgcgcctg cgaagcgcct
    12901 cattccacgc tcctgttgac cacctggtct taaccggaat ttgaatgcct gtagtcgttt
    12961 catgggccaa atcatacttt ggcctatgaa aaaagaaacc gatatccgac gcggaaggca
    13021 ttgtgttttc ctgaagcatg ttcacctggt cttcgttact aaataccgac gtcagatttt
    13081 tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
    13141 agctgaacgg gttgaaatgg atggcggacc agatcacgtc catttgttaa ttaactatcc
    13201 tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcga gtaggttact
    13261 gcgacgagat cgaccagata ttgcagtcag gtattacgac aaaggcgttt tgtggagtcc
    13321 tggctatttt gtcagtagct gcatcttaac gtatcaacta gatttaataa caacgatatc
    13381 cgtacctaac cggatatcta atgtcataag catcccttta attatcccct caacctcttg
    13441 taactttccc actcttgagc tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt
    13501 gcatttctga aattgtgatg gaggaagata gtatcaatta tatgtgctta gaaacgtccc
    13561 cgattagcgc agaagtcacc ggattgttca gactccaata ggttgagtat gccgtattta
    13621 ttttccaaaa taaaaatcga tgctgttgct ccttcataaa atacttttac tctctggacg
    13681 cttttcttag ctctcttggt gttatcttaa aacgattatg gaacctttca gtaaaacgag
    13741 aaggacactt gtaaccattt tccctggcaa tctcgctgat gggttttact gtcgtttgta
    13801 tagcagacaa cgcattattt aacctcacgt cgtccaatat actttgaaaa cttactccct
    13861 cgcttgccag acggcgatgc aatgtggaaa cagaaatgta gagatatctc gctaccttgt
    13921 ttgctgtcca ttttgttcca ggttcggata gcattaggtt ataacaacga cttattaatg
    13981 attgtttact atctgataaa agtaaacaat taacatgatg tgctcctaat gaaagtagaa
    14041 cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc tcgggaaata gatgttttta
    14101 gttgctccca acaatatatt aactcggggt tctgaggt
//
LOCUS       DACADA010000118        14093 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.120, whole genome shotgun sequence.
ACCESSION   DACADA010000118 DACADA010000000
VERSION     DACADA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.120"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            254..532
                     /locus_tag="ISB17_003449"
                     /pseudo
     CDS             254..532
                     /locus_tag="ISB17_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(583..828)
                     /locus_tag="ISB17_003450"
                     /pseudo
     CDS             complement(583..828)
                     /locus_tag="ISB17_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1201..1431)
                     /locus_tag="ISB17_003451"
                     /pseudo
     CDS             complement(1201..1431)
                     /locus_tag="ISB17_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2170..2616)
                     /locus_tag="ISB17_003452"
                     /pseudo
     CDS             complement(2170..2616)
                     /locus_tag="ISB17_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(2803..2887)
                     /locus_tag="ISB17_003453"
     tRNA            complement(2803..2887)
                     /locus_tag="ISB17_003453"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2851..2853),aa:Leu,seq:caa)
     gene            3083..4102
                     /gene="ahr"
                     /locus_tag="ISB17_003454"
     CDS             3083..4102
                     /gene="ahr"
                     /locus_tag="ISB17_003454"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAP1981346.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVI
                     LKADF"
     gene            4232..5734
                     /locus_tag="ISB17_003455"
     CDS             4232..5734
                     /locus_tag="ISB17_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAP1981347.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPMTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5895..6977)
                     /gene="lptG"
                     /locus_tag="ISB17_003456"
     CDS             complement(5895..6977)
                     /gene="lptG"
                     /locus_tag="ISB17_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP1981348.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQIDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6977..8077)
                     /gene="lptF"
                     /locus_tag="ISB17_003457"
     CDS             complement(6977..8077)
                     /gene="lptF"
                     /locus_tag="ISB17_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP1981349.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8344..9855
                     /gene="pepA"
                     /locus_tag="ISB17_003458"
     CDS             8344..9855
                     /gene="pepA"
                     /locus_tag="ISB17_003458"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAP1981350.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10211..10654
                     /gene="holC"
                     /locus_tag="ISB17_003459"
     CDS             10211..10654
                     /gene="holC"
                     /locus_tag="ISB17_003459"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAP1981351.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10654..13509
                     /gene="valS"
                     /locus_tag="ISB17_003460"
     CDS             10654..13509
                     /gene="valS"
                     /locus_tag="ISB17_003460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP1981352.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13563..>14093)
                     /locus_tag="ISB17_003461"
     CDS             complement(13563..>14093)
                     /locus_tag="ISB17_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAP1981353.1"
                     /translation="TTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDI
                     ENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTY
                     HGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKG
                     FLASISRGIMPSLPFL"
BASE COUNT         3454 a         3645 c         3771 g         3223 t
ORIGIN      
        1 ttgatggtag ctaacttagt aattatataa aaaactttta caatatccga gagtatgtat
       61 gcataatgta tgcgcccccc tatcaggctg tctcttatac acaaatcccc acccggggat
      121 ttgtgatgtc tgtcagtctg ctttaggggg gactctttcc attcggtagt ttccttcctc
      181 cccggcagcg attttttgaa ctttttcttc acccgttgat cttctcttat atttcatttt
      241 cttcgggtct gttatggtac tttctgattg ttattcatgg gataacgagc agtttggaca
      301 tgcccgtctc ggtgatccgc gtcgtacacg acgccttgtg tcactggcct cttctctcgc
      361 tcagcatgca ggtctttcta ttgtgaaatc atcccactcc actgctcagg ttgaaagtgc
      421 gtaccgcctg atacgtaacc cgtctgtgtc cccggaagcc attgcctaag ccggatttac
      481 cgccactgca agggcatgtg aggctcatcc gctgctcctc gcactggaag acaccaccac
      541 taatcgtaag cgtacagcga gggccgtatt gacggggatg tgttattcag ctggcagtgc
      601 tatacgccac ggaagcagtt cgctgacccg gttgaccggc cagtctgcta tgacgccaag
      661 cacatggcga aggtagcttt ctggatccac gtcattcagt ttgcacgtcc cgatcaggct
      721 gtacagtagc gctccccgct caccaccatg gtcagagccg aagaacagga agtttttacg
      781 acccagactg accgtcaacg ccacaatctt tgactgtttg acattgtcat gcccgcaggc
      841 cagcgaatct aaagacatca gactgcttgc atcagccagt tgcgtatctg gctatgtccc
      901 ttaattggta agggttcgag gaactcaaca ttaacgcatt tttccgccca tcaccggcag
      961 tgtatatgaa cgattgcact cttacgatgg ttgatttatt tggttgcgta ggggctagct
     1021 agtctggtac cggaggctgg atctgaagta ctgaccccaa gaataacgcc gggcagactc
     1081 atattctgac ccattgtcag cccgcgtgtt tactgctcta gctcatttcc tgttgtcagg
     1141 cgttcaattc ccaactgcag gttatggccc ggtgcacaaa cacctgacgg caggtatcac
     1201 ttacccaaag aagataaagc cctcaagagg cagtaactct gccaacctct cctccggcca
     1261 ctccgggcag acgcgttagg acgtccgtca gccaagcatg tggctccaga ctgctgtttc
     1321 cagaaggctc attatctgtg ccgctcggtt cccggccatc agtgaaccag cgaacaacca
     1381 ggcctttcgt cccataacga ccggacggat agcacgctcg cagatattgt tgtgtgtgcc
     1441 ttgaaatgca cgatctttcc gtcggtgaaa gtccgccccg actaactctc gctccaactg
     1501 gaaggacagg aagcggtcca ggaggtaaca aaagggctga atctctccct gaaacgactt
     1561 cttcaggaag tcaatgggtt atgcgggccg taacgtgagt gaacactgag cagccatcga
     1621 aatgccgtcg atggtgccga tacgccacaa taacggggaa ggccgacgat gaccgggaag
     1681 agagtgagaa aggcatcggt catacccacc ggggtagtgg tgtcagcatg cataacaatt
     1741 gatcgatcgt gcaacaagag aagtccataa cgatgcctgt ccgggtgacg ggcaacaacg
     1801 cctcccgagg gggtgaatcg gttgttatgg atggcagatg gggtgtagta gtgaaaatac
     1861 tgagtaatgt cataggagcg aaggccccct gttgcagtac cagtgtaaca tgcaggaaga
     1921 gcccggggat tgccatcagc ctcacaaccc cgctcgaacc ggttcagaac ctacagaaaa
     1981 tactacagga gaaagcaaag tgaacctccc cgtgtttcct gtttatcatt ttctggtcag
     2041 tgccgcgatt ctggtattcg tggtgatttt ctggcggaca caccatcgcg accaccgtaa
     2101 ctggctggct ctacggctat tcgtattatg ctctgtaaac cgttggccat tgcggatggt
     2161 taaaggaaca ataggtgcta ataaatctcg agtttttaac tcagcgatat tacgttcacc
     2221 aagcgctgca aaaagattat cttcaagact ttttagcaca cgatgggcgt gatcttcaga
     2281 ccacttttta ttggtgccat gccactcaat cgcgacttct ttaaaggttc gtgctttact
     2341 ctgttcaacc ttatcatttt tctttttgtc tcccggatcg acgccattcg caagcagctt
     2401 acgcgcctcg tcacgacgta ctctggcatc cgctagtgtg atttcaggat aaaccccaag
     2461 tgccagcatt ttttgctttc cctcataacg gtactgcaaa cgccagtact tagaaccatt
     2521 tggatggaca agcagatgca taccgccacc atcagtcagc ttatattgct tatccgttgg
     2581 cttggctgct ctgactttga tatctgttaa tgccatgcgt attccctccc tgttggtaca
     2641 agcattatcg aaccggaaat accatcatct gtaccaacat atgttggtag atgtacgttg
     2701 atatcggttg accttgagag agttaatata gcgggaaaga tagataaaca ctggatttca
     2761 ggcataaaaa aagacctcag ttgaggtcta tttacatact tttggtgccg aaggccggac
     2821 tcgaaccggc acgtatttct acggttgatt ttgaatcaac tgcgtctacc gatttcgcca
     2881 cttcggcact gaagaggtat gcggaaaacg ttgtggatta tacctgtcgc acgccaccat
     2941 gcaagcgcca gcttgctcgc acctcgctaa gtgctgaaat tttcagcatt gcatacagcg
     3001 atgtgtaacc tttgtcacac tccaggcacc ccgccctgcc atgctctaca cttcccagac
     3061 aacaccagag aaggaccaaa aaatgtcgat gataaaaagc tatgccgcaa aagaagcagg
     3121 cggcgaactg gaagtttatg agtacgatcc cggtgaactg aagccacaag atgttgaagt
     3181 gcaggtggat tactgcggga tctgccattc cgatctgtcg atgatcgata acgaatgggg
     3241 attttcacaa tatccgctgg ttgccgggca tgaggtgatt ggtcgcgtgg tggcgctcgg
     3301 gagcgccgcg caggataaag gtttgcaggt cggtcagcgt gtcgggattg gctggacggc
     3361 gcgtagctgc gggcattgcg atgcctgtat tagcggtaat cagatcaact gtgagcaagg
     3421 tgcggtgccg acgattatga atcgcggtgg ctttgccgag aagttgcgtg cggactggca
     3481 atgggtgatt ccactgccag aaaatattga tatcgagtcc gccgggccgc tgttgtgcgg
     3541 tggtatcacg gtcttcaaac cactgttaat gcaccatatc actgccacca gccgcgttgg
     3601 ggtaattggt attggcgggc tggggcatat cgctataaaa cttctgcacg caatgggatg
     3661 cgaggtgaca gcctttagtt cgaatccggc gaaagagcag gaagtactgg cgatgggtgc
     3721 cgataaagtg gtgaatagcc gcgatccgca ggcactgaaa gcactggcgg ggcagtttga
     3781 tctcattatc aacaccgtca acgtcagcct cgactggcag ccttattttg aagcgctgac
     3841 gtacggcggt aatttccata cggtcggtgc ggttctcacg ccgctgtctg ttccggcctt
     3901 tacgttaatt gcgggcgacc gcagcgtctc tggttctgct accggcacgc cttatgagtt
     3961 gcgtaagctg atgcgctttg ccgcccgcag caaggttgcg ccgactaccg aattgttccc
     4021 gatgtcgaaa attaacgacg ccattcagca tgtacgcgat ggtaaagcgc gctaccgagt
     4081 gatcctgaaa gccgacttct gatccctttg ccgggtgcct gtcgcccggc acttaaatct
     4141 ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcgca gccgttgcct
     4201 atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa ttgcccgcac
     4261 gccggacaca gaactatttt tactgccggg aatggctaac cgtcacgggc tgattactgg
     4321 cgcaacgggg acgggtaaaa ctgtcacgct gcaaaaactg gcggagtctc tatcggaaat
     4381 cggtgttccg gtgtttatgg ccgatgtgaa aggcgatctg accggcatcg cgcaggcagg
     4441 aacggcgtcg gaaaaactgc tcgcaaggct taaaaatatc ggcgtcaatg actggcaacc
     4501 gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccatc cggtgcgggc
     4561 gacggtttcg gatctggggc cgctgttgtt ggcgcggctg ttgaatctca acgatgtgca
     4621 gtctggcgtg ctggatatca tcttccgcat tgctgacgat caggggctgt tactgctcga
     4681 ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat ctttccagaa
     4741 tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcgggc tgttgtcgct
     4801 ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca aagactggat
     4861 gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga agctttatca
     4921 gatgccgaaa ctgtatgccg ccagcctgtt gtggatgctc tcggagttgt atgaacaatt
     4981 gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg aggcacatct
     5041 gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga taaggcttat
     5101 tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaat ccgtccgata ttccggataa
     5161 cgtgctcggc cagctcggta atcgcgttca acacgctttg cggactttta cgcccaaaga
     5221 tcagaaagcg gtaaaagctg cggcgcaaac catgcgggtc aatccggcat ttgataccga
     5281 aaaggcgatt caggaactgg gcaccggcga ggcgttgatc tcttttctgg atgcgaaagg
     5341 aagcccttct gtggtggaac gggcgatggt gattgcgcct tgttcgcgga tgggaccgat
     5401 gacggaagat gagcgtaatg gcttgattaa tcactctccg gtgtatggca agtacgagga
     5461 tgacgtggac cgggaatccg cctatgagat gttgcaaaaa ggcgtacagg caagcgccga
     5521 gcagcaaaat aatcctgccg tgaaagggaa agaggtggcg gtggatgacg gcattcttgg
     5581 tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga aagatggtgt
     5641 ggtgcaaaca atggcgaaaa gcgccgcccg ccaggtgacg aatcagattg tacgtgggat
     5701 gttggggagt ttgctggggg gaagaagaag gtaagtcagg cgcgatttgt ctttgcctga
     5761 tgcgacgctg gcgcgtctta tcatgcctac accactgcaa tatattggat tttcatgatt
     5821 tttcaggcat gaacgaagca cactttgcta acaataataa agggagcttt cgctcccttt
     5881 attcgttcat tcggttacga ttttctcatt aacagccaca ggctgattaa gaagaagctg
     5941 gcgcttggca acagtgcgcc gatgatcggc gggatgccat aaaccaacgt cagcgggccg
     6001 aagatctggt ccagtacata gaagacaaaa ccgaagctga taccggtgac cacacgcaca
     6061 cccatcggta cgctacgcag tgggccaaag atgaacgaca gcgccatcag catcatcacc
     6121 gccacggata gcggctggaa gattttgctc cacatgttga gctgataacg tccggcatcc
     6181 tgaccgctcg atttcagata cttcacatag ttgtgcaaac cgctgataga gagtgcatcc
     6241 ggatccagcg ccaccacgcc cagtttgtca ggcgtgaggt tggttttcca ggtgccgctc
     6301 accgtctgcg aaccggtgat ctgtttcgga ttggtcagat cagattcatc aatctgcgac
     6361 agacgccaga ctttatgttc cgggtcaaac ttcgcggtag cggcatagcg tacggattgc
     6421 agacgacgat tctcgttaaa ggcataaata ctgatgccac ctaactcttc gtcaccttta
     6481 acccgctcaa tgtagacgaa gttgttgcca tctttcgccc ataaaccttg ctgggtagag
     6541 agcaacgagc cgccgtacat cgcctgcgca cggtagttac gcgccatctg ctcgccctgc
     6601 ggcgcgaccc attcgccaat cgccatcgtc agcaagacca gcggaatggc ggttttcatc
     6661 accgacagcg ccacctgcat acgggtaaaa ccagaagcct gcatcaccac cagttcgctg
     6721 cgctgcgcca gcatcccaag accaagcaac gccccaagca aagccgccat cgggaagaat
     6781 atttgcacat ctttcggcac gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtag
     6841 ctcccctgcc cggctttttt caactgatcg acaaatttga taatgcccga cagcgacacc
     6901 agcatgaaca gcgtcatcat gatggtggtg aaaatagttt taccgatata gcggtcaagt
     6961 acgccaaaag gttgcatcac accgctcctt tacgcgaaaa actggcgcgc aggcggcgga
     7021 ccggcacagt gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc aggttaacgg
     7081 tccacatcca cagcgtcggg tccagcttac ctttaccgcc gttcgatttc agggaggtct
     7141 ggatcaggaa gaaaagtaga tacagcagca tggctggcag catcgacagt acgcgtccct
     7201 gacgcgggtt aaccacgctc aacggtacaa ccataagtgc catcataaac acggtgaata
     7261 ccaacgtgat acgccagttc agttctgcgc gggcacgatc ggtgtcagtg ttccacaatg
     7321 tgcgcatgtc catctggtcg gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga
     7381 tcgcctgata atcctggaaa tccgtaatgc ggaaatcacg taacagtgca gtgccttcga
     7441 agcgcgttcc ctggttgaga gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc
     7501 cggaatcggc caccaccaca gaaggtcgtg cattaccttt tggtcgaatt tgtgcgagga
     7561 acacatcttt gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag
     7621 tcgcttgctg gaattgccct tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg
     7681 ctaacacttc atcctgatga cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg
     7741 cgacgattgc cgtgaatacc gcaaggatca ttgccgcttt caccagaacc gctttgctca
     7801 ggccgcaggc atgcattacc gtaatttcac tttcggtata cagtttgccc agcgtcatca
     7861 gcagcccgag gaacaggctt aatggcagga taagctgcgc catttccggc acgcccaacc
     7921 cgagaaggga gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc
     7981 tcactaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt
     8041 tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc
     8101 gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt
     8161 cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg
     8221 tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt
     8281 acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag
     8341 tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc
     8401 gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc
     8461 gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca
     8521 ttgttgctgc accatgtacc aaacgtgctt tctgaacgca tcctccttat tggttgcggc
     8581 aaagaacgtg agctggatga gcgtcagtac aagcaggtta ttcagaaaac cattaatacg
     8641 ctgaatgata ctggctcaat ggaagcggtc tgctttctga ctgaactgca cgttaaaggc
     8701 cgtaacaact actggaaagt gcgtcaggct gtcgagacgg caaaagagac gctctacagt
     8761 ttcgatcagc tgaaaacgaa caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc
     8821 aacgtgccga cccgccgtga actgaccagc ggtgagcgcg cgatccagca cggtctggcg
     8881 attgccgccg ggattaaagc agcaaaggat cttggcaata tgccgccgaa tatctgtaac
     8941 gccgcttacc tcgcttcaca agcgcgccag ctggctgaca gctacagcaa gaatgttatc
     9001 acccgcgtta tcggcgaaca gcagatgaaa gagctgggga tgcattccta tctggcggtc
     9061 ggtcagggtt cgcaaaacga atcgctgatg tcggtgattg agtacaaagg caacgcgtcg
     9121 gaagatgcac gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc cggcggtatc
     9181 tcgatcaagc cttcagaagg catggatgag atgaagtacg atatgtgcgg tgcggcagcg
     9241 gtttacggtg tgatgcgtat ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg
     9301 ttggcaggct gcgaaaacat gcctggcgga cgagcctatc gtccgggcga tgtgttaacc
     9361 accatgtccg gccaaaccgt tgaagtgctg aatactgacg ctgaaggccg cctggtactg
     9421 tgcgacgtgt taacttacgt tgagcgtttt gagccggaag cggtgattga cgtggcgacg
     9481 ctgaccggtg cctgcgtgat cgcgctgggt catcatatta ctggtctgat ggcgaaccat
     9541 aatccgctgg cccatgaact gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc
     9601 ttaccgctgg gtgacgagta tcaggaacaa ctggagtcca attttgccga tatggcgaac
     9661 attggcggtc gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg ctttacccgt
     9721 aagtacaact gggcgcacct ggatatcgcc ggtaccgcct ggcgttctgg taaagcaaaa
     9781 ggcgcaaccg gtcgcccggt ggcgttgctg gcacagttcc tgttaaaccg cgctgggttt
     9841 aacggcgaag agtaattgcg tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct
     9901 tatccgacct acacagcact gaactcgtag gcctgataag acgcgcaagc gtcgcatcag
     9961 gcaatgctgt tattgtcgga tgcggcatga acgccttatc cgacctacac acagcactga
    10021 actcgtaggc ctgataagac acaacagcgt cgcatcaggc aatgctgtta ttgtcggatg
    10081 cggcgtgagc gccttatccg acctacacac agcactgaac tcgtaggcct gataagacac
    10141 aacagcgtcg catcaggcgc tgcggtgtat acctgatgcg tatttaaatc caccacaaga
    10201 agccccattt atgaaaaacg cgacgttcta ccttctggac aatgacacca ccgtcgatgg
    10261 cttaagcgcc gttgagcaac tggtgtgtga aattgccgca gaacgttggc gcagcggtaa
    10321 gcgcgtgctc atcgcctgtg aagatgaaaa gcaggcttac cggctggatg aagccctgtg
    10381 ggcgcgtccg gcagaaagct ttgttccgca taatttagcg ggagaaggac cgcgcggcgg
    10441 tgcgccggtg gagatcgcct ggccgcaaaa acgcagcagc agcccgcgcg atatattgat
    10501 tagtctgcga acaagctttg cagattttgc caccgctttc acagaagtgg tagacttcgt
    10561 tccttatgaa gattctctga aacaactggc gcgcgaacgc tataaagcct accgcgtggc
    10621 tggtttcaac ctgaatacgg caacctggaa ataatggaaa agacatataa cccacaagat
    10681 atcgaacagc cgctttacga gcactgggaa aagcagggct actttaagcc taatggcgat
    10741 gaaagccagg aaagtttctg catcatgatc ccgccgccga acgtcaccgg cagtttgcat
    10801 atgggtcacg ccttccagca aaccatcatg gataccatga tccgctatca gcgcatgcag
    10861 ggcaaaaaca ccctgtggca ggtcggtact gaccacgccg ggatcgctac ccagatggtc
    10921 gttgagcgca agattgccgc agaagaaggt aaaacccgtc acgactacgg ccgcgaagct
    10981 ttcatcgaca aaatctggga atggaaagcg gaatccggcg gcacgattac ccgtcagatg
    11041 cgccgtctcg gcaactccgt cgactgggag cgtgaacgct tcacgatgga cgaaggcctg
    11101 tccaatgcgg tgaaagaagt tttcgttcgt ctgtataaag aagacctgat ttaccgtggc
    11161 aaacgcctgg taaactggga tccgaaactg cgcaccgcta tctctgacct ggaagtggaa
    11221 aaccgcgaat cgaaaggttc gatgtggcac atccgctatc cgctggctga cggcgcgaaa
    11281 accgcagacg gtaaagatta tctggtggtt gcgactaccc gtccggaaac cctgttgggc
    11341 gataccggcg tggccgttaa cccggaagat ccgcgttaca aagacctgat tggcaaatat
    11401 gtcattctac cgctggttaa ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg
    11461 gaaaaaggca ccggctgcgt gaaaatcact ccggcgcacg actttaacga ctatgaagtg
    11521 ggtaaacgtc acgcgctgcc gatgatcaac atcctgacct ttgacggcga tatccgtgaa
    11581 agcgcccagg tgttcgatac caaaggtaac gaatctgacg tttattccag cgaaatcccg
    11641 gcagagttcc agaaactcga gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac
    11701 gcactcggcc tgctggaaga aattaaaccg cacgacctga ccgttcctta cggcgaccgt
    11761 ggcggcgtag ttatcgaacc aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg
    11821 gcgaaaccgg cggttgaagc ggttgagaac ggcgacattc agttcgtacc gaagcagtac
    11881 gaaaacatgt acttctcctg gatgcgcgat attcaggact ggtgtatctc tcgtcagctg
    11941 tggtggggtc accgtatccc tgcatggtac gacgaagcag gtaacgttta tgttggccgc
    12001 aacgaagaag aagtgcgcaa ggaaaataac ctcggtgccg acgttgccct gcgtcaggac
    12061 gaagacgttc tcgacacctg gttctcttct gcactgtgga ccttctctac ccttggctgg
    12121 ccggaaaaca ccgacgccct gcgtcagttc cacccaacca gcgtgatggt atccggcttc
    12181 gacatcatct tcttctggat tgcccgcatg atcatgatga ccatgcactt catcaaagat
    12241 gaaaatggca aaccgcaggt gccgttccac actgtttaca tgaccggtct gattcgtgac
    12301 gacgaaggcc agaagatgtc caaatccaag ggtaacgtta tcgacccgct ggatatggtt
    12361 gacggtattt cgctgccaga actgctggaa aaacgtaccg gcaatatgat gcagccgcag
    12421 ctggcggaca aaatccgtaa gcgcaccgag aaacagttcc cgaacggcat tgagccgcac
    12481 ggcaccgatg ccctgcgctt caccctggcg gcgctggcgt ctaccggtcg tgacatcaac
    12541 tgggatatga agcgcctgga aggttaccgt aacttctgta acaagctgtg gaacgccagc
    12601 cgctttgtgc tgatgaacac ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg
    12661 ctgtcgctgg cggaccgctg gattctggca gagttcaacc agaccatcaa agcgtaccgc
    12721 gaagcgctgg acagcttccg cttcgatatc gctgcaggca ttctgtatga gttcacctgg
    12781 aaccagttct gcgactggta tctggagctg accaagccgg taatgaacgg tggaaccgaa
    12841 gccgaactgc gcggtactcg ccatacgctg gtgactgtac tggaaggtct gctgcgcctc
    12901 gcgcatccga tcattccgtt catcaccgaa accatctggc agcgtgtgaa agtactttgc
    12961 ggtatcactg ccgacaccat catgctgcag ccgttcccgc agtacgatgc atctcaggtt
    13021 gatgaagcgg cgctggccga caccgagtgg ctgaagcagg cgatcgttgc ggtacgtaac
    13081 atccgtgcag aaatgaacat cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc
    13141 agcgcggatg cagaacgtcg cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt
    13201 ctggaaagca tcaccgtgct gcctgccgat gacaaaggtc cggtttccgt tacgaagatt
    13261 atcgacggcg cagagctgct gatcccgatg gctggcctca tcaacaaaga agatgagctg
    13321 gcgcgtctgg cgaaagaagt ggcgaagatc gaaggcgaaa tcagccgcat cgagaacaag
    13381 ctggcgaatg aaggctttgt cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag
    13441 aagctggaag gctatgcgga agcgaaagcg aagctgattg aacagcaggc tgttatcgcc
    13501 gcgctgtaat caacaccgtg atgaaaacga aggccggagc atgctccggc cttttttatc
    13561 tcttacagaa atggtaaaga aggcataatt ccccgtgaaa tactggctaa gaagccttta
    13621 tctggttgtt cttctttatt gattaaagga aggtcatcca aatcgcccag caaatacgta
    13681 ttttttgcct gccagtcaat catccatatt ttcagcagtg gataagccag accgcccgta
    13741 atcccggata tcaccaccaa cgcacacatg cgataaagca taccgtggta tgttaaagtt
    13801 gagcgaaaac gaatacgccc atcattcagt gacaggttgc tcataaaatg atttcgcaac
    13861 gacaccgtta aataactggt gctaacagca atcccaaaat aatagattaa ctgcgcgatt
    13921 atcattttac gttgcatttc cataaattgc tgtaaattct caatatcatc atttgcatac
    13981 acagaactat catacgcatt taatatttga tcgaagataa tataaccagc aacagcgaga
    14041 aaaggtaata aagctaaaaa tgcgagaatg gcatatttta tacaatacgt agt
//
LOCUS       DACADA010000119        14057 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.121, whole genome shotgun sequence.
ACCESSION   DACADA010000119 DACADA010000000
VERSION     DACADA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.121"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..646
                     /gene="gspC"
                     /locus_tag="ISB17_003462"
     CDS             <1..646
                     /gene="gspC"
                     /locus_tag="ISB17_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HAP1981354.1"
                     /translation="SEFSLAALWRNENHTGVKDANPVAVNQETPKLSIALNGIVLTSN
                     DETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPD
                     IIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINN
                     LSLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            630..2582
                     /gene="gspD"
                     /locus_tag="ISB17_003463"
     CDS             630..2582
                     /gene="gspD"
                     /locus_tag="ISB17_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HAP1981355.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2592..4073
                     /gene="gspE"
                     /locus_tag="ISB17_003464"
     CDS             2592..4073
                     /gene="gspE"
                     /locus_tag="ISB17_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HAP1981356.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4070..5266
                     /gene="gspF"
                     /locus_tag="ISB17_003465"
     CDS             4070..5266
                     /gene="gspF"
                     /locus_tag="ISB17_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HAP1981357.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5276..5713
                     /gene="gspG"
                     /locus_tag="ISB17_003466"
     CDS             5276..5713
                     /gene="gspG"
                     /locus_tag="ISB17_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAP1981358.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5721..6230
                     /gene="gspH"
                     /locus_tag="ISB17_003467"
     CDS             5721..6230
                     /gene="gspH"
                     /locus_tag="ISB17_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAP1981359.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6227..6604
                     /gene="gspI"
                     /locus_tag="ISB17_003468"
     CDS             6227..6604
                     /gene="gspI"
                     /locus_tag="ISB17_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HAP1981360.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6597..7184
                     /gene="gspJ"
                     /locus_tag="ISB17_003469"
     CDS             6597..7184
                     /gene="gspJ"
                     /locus_tag="ISB17_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAP1981361.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7177..8160
                     /gene="gspK"
                     /locus_tag="ISB17_003470"
     CDS             7177..8160
                     /gene="gspK"
                     /locus_tag="ISB17_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAP1981362.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8175..9338
                     /gene="gspL"
                     /locus_tag="ISB17_003471"
     CDS             8175..9338
                     /gene="gspL"
                     /locus_tag="ISB17_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAP1981363.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9335..9796
                     /locus_tag="ISB17_003472"
     CDS             9335..9796
                     /locus_tag="ISB17_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HAP1981364.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9796..10468
                     /locus_tag="ISB17_003473"
                     /pseudo
     CDS             9796..10468
                     /locus_tag="ISB17_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(10497..10973)
                     /gene="bfr"
                     /locus_tag="ISB17_003474"
     CDS             complement(10497..10973)
                     /gene="bfr"
                     /locus_tag="ISB17_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAP1981365.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11045..11239)
                     /gene="bfd"
                     /locus_tag="ISB17_003475"
     CDS             complement(11045..11239)
                     /gene="bfd"
                     /locus_tag="ISB17_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAP1981366.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11408..>14057)
                     /gene="chiA"
                     /locus_tag="ISB17_003476"
     CDS             complement(11408..>14057)
                     /gene="chiA"
                     /locus_tag="ISB17_003476"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HAP1981367.1"
                     /translation="SLPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRA
                     ATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQE
                     GSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNPGSC
                     SVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIY
                     HVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEIDNG
                     GVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSAS
                     TVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKTQSYSNEELN
                     NAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPK
                     KAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQ
                     NYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDF
                     DIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDA
                     KAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHAQLKGLYGNKSDA
                     EIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLS
                     PEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3607 a         3621 c         3344 g         3485 t
ORIGIN      
        1 gtctgaattc agccttgccg ctttatggcg caacgaaaat catactgggg taaaagatgc
       61 aaatcccgtg gcagttaatc aggaaacacc taagctctct atagcgctta atggcattgt
      121 actcacatcg aatgatgaga cttcttttgt cttaattaat gaggggaacg aacaaaagcg
      181 atactccctc aacgaagccc ttgagtcagc gccgggaacg tttattagaa aaataaataa
      241 aaccagcgtt gtgtttgaaa ctcacgggca ttacgaaaaa gtaactctcc atccagggct
      301 acctgacatc atcaagcagc ctgattcgga aaatcaaaat gtactcgccg attatattat
      361 cgccacacct atacgtgatg gagaacagat atacggtctt cgattgaatc ctcgtaaggg
      421 tctcaatgca tttaccacca gtttgctgca acctggggat atcgccctca ggatcaataa
      481 tctttcgctg acccaccctg atgagatttc acaggcatta agtttactgc taactcaaca
      541 aagtgctcag tttacaattc gtcgcaacgg cgtaccccgc ttgataaatg tttccgtcgc
      601 ggaacttaca ggaatgaatg gacagcgcaa tgaagggact caataaaatc acctgctgct
      661 tgctggcagc attactcatg ccttgtgcag gacacgctga gaacgaacaa tacggcgcta
      721 acttcaataa cgccgatatc cgccagttcg tggaaatagt gggtcagcat cttggcaaaa
      781 cgatcctgat cgacccttcg gtacagggaa ccatttccgt acgcagtaat gatacgttta
      841 gccaacagga gtactaccag ttctttttaa gtattcttga tctttacggt tattccgtga
      901 tcacgctgga caatggtttt ctgaaagtgg tgcgctcagc taatgtaaaa acatcgccag
      961 ggatgattgc tgacagttct cgtccaggcg taggtgatga gttggtcacc cgaatcgtac
     1021 cgcttgagaa cgttcctgct cgtgacctgg ctcccctgct ccgccagatg atggatgcgg
     1081 gtagcgtcgg taatgttgtg cattatgaac cctccaacgt tcttattctg accggtcgtg
     1141 cctccaccat taataaactg attgaagtca taaagcgcgt tgatgtcatc ggcacagaga
     1201 agcagcaaat tattcatctg gaatatgcat cagcggaaga tctcgccgag attcttaatc
     1261 aattaatcag cgaaagccac ggtaaaagcc agatgcctgc cctcctttcc gcgaagattg
     1321 tggcggataa gcgaaccaac tctcttatca tcagcggacc ggaaaaagca cgccagcgca
     1381 taacttcatt actgaaaagc cttgatgtag aagagagcga ggaaggaaat acccgggtct
     1441 attacctgaa atatgctaaa gccacgaatc tggtggaagt gctaaccggt gtttccgaaa
     1501 agctgaaaga tgaaaaaggg aattcgcgta agccttcttc tacttctgcg atggataatg
     1561 tggccattac cgccgatgaa cagaccaact cgttggtcat taccgctgac cagtccgtcc
     1621 aggagaaact cgccacggta attgcgcgtc tggatattcg ccgtgcacag gtgctggttg
     1681 aggcaatcat cgtcgaagtt caggatggaa atggactcaa cctcggcgtg caatgggcga
     1741 ataaaaacgt tggcgcacag caatttacca atacgggatt accggttttt aacgctgcgc
     1801 aaggtgtagc tgattataaa aagaatggcg ggatcaccag cgcgaatcct gcctgggata
     1861 tgtttagcgc ctacaatggc atggctgcag gcttcttcaa tggcgactgg ggcgtattgt
     1921 tgactgcgct ggccagtaac aataaaaatg acatcctcgc caccccgagc atcgtaacgc
     1981 tggataataa actcgcgtcc ttcaacgttg gtcaggatgt gccggtgcta tccggctctc
     2041 agaccacttc tggggataac gtctttaata ctgtcgaacg caaaacggtg ggcacaaaac
     2101 tcaaagtcac tccgcaggtc aatgaaggcg acgcggtact gctcgaaata gaacaggagg
     2161 tttccagcgt tgactcttcg tctaattcga cgctcggccc gacatttaac acccgtacta
     2221 ttcaaaacgc cgtgctggtt aaaaccggtg aaacggtggt cctgggcgga ttgctggatg
     2281 atttttctaa agagcaagtg tcaaaggttc ctctgcttgg cgatattcct ttagtgggac
     2341 aactcttccg ctatacctcg accgagcgcg ctaaacgcaa cctgatggta ttcatccgcc
     2401 cgacgattat ccgtgacgat gatgtttatc gctcactgtc aaaagagaaa tacacccgct
     2461 accgtcagga gcaacagctg cgaatcgacg ggaaatcaaa agcgctggtt ggctcggaag
     2521 atttgccggt gctgaatgag aacacgttca acagtcacgc ccctgcgcca tcgtcacggt
     2581 gaggcattct tatgagaatt cactcaccgt accccgccag ttgggcgctg gcacaacgaa
     2641 ttggttatct ctattcagag ggcgagatta tttatctcgc cgatacgcca ttcgaacggt
     2701 tactcgatat tcaacgtcaa gttggtcagt gccagaccat gaccagcttg tcacaggctg
     2761 attttgaagc ccgactggaa gcggtgttcc atcagaatac cggtgagtcg caacagattg
     2821 cgcaggatat cgatcaatcc gtcgatcttc tctcgctttc ggaagagatg cccgcaaatg
     2881 aagatctcct gaatgaagat tcagcggcac cggttatccg cttgatcaat gctattttga
     2941 gtgaggccat caaagaaacc gcctccgata tccacattga aacctatgaa aaaacaatgt
     3001 cgatccgttt tcgcatcgac ggcgttttgc ggacaatttt acagcccaac aaaaaactgg
     3061 cggcactgct tatctcccga attaaggtca tggctcgtct tgatatcgcc gaaaaacgta
     3121 ttccacagga tggaaggatc agtttgcgta tcgggcgacg taacatagat gtccgcgtat
     3181 ccacactgcc atccatctat ggtgaacgcg ctgtgctccg tctgctggat aaaaacagcc
     3241 tccagctttc attgaacaac ctggggatga cggcagcgga taaacaggat ttagaaaatc
     3301 tcattcagct tccgcacggt attatcctgg tgacagggcc gacaggctcc ggtaaaagca
     3361 ccacgctcta cgccatcctt tcggcgctta atatccctgg ccgtaatatt ctgacggtag
     3421 aagatcccgt ggaatatgag ctggaaggca ttggacaaac gcaggtgaat acccgtgtgg
     3481 atatgtcttt cgctcgcggc ctgcgcgcca tacttcgcca ggacccggat gtcgtcatgg
     3541 tgggggaaat tcgtgataca gaaaccgcgc aaattgcggt tcaggcctcg ctcacaggcc
     3601 atctggtact ctcaacactc cacactaaca gtgcatcagg cgcagtcacc cggctacgcg
     3661 acatgggcgt cgaatcattc ctgctttcat cttcactggc agggattatc gcgcaacgtc
     3721 tggttcgtcg tctgtgtccg caatgccgac aattcacgcc cgtatcgccc cagcaagcgc
     3781 agatgtttaa atatcatcag ctcgcggtga caacaattgg cactcccgta ggttgccctc
     3841 attgccatca atccggttat caggggcgca tggcgatcca cgaaatgatg gtggtgacgc
     3901 cggaattgcg ggccgctatt catgaaaatg tggatgaaca agcactggag cgactggtcc
     3961 ggcaacaaca caatgcctta atcaaaaatg gcctgcaaac agtgatacgc ggtgacacct
     4021 cctgggatga ggttatgcgc gtcgccagtg ctacgctgga gaacaaagca tgaattatcg
     4081 ctatcgcgcc atgacccagg atggtcaaaa attgcagggg atcattgatg ctaacgatga
     4141 acgtcaggca cgactgcggc tgcgtgaaga agggcttttc ctgttggata ttcgccccca
     4201 aaaaagttcg ggagtaaaaa cacgtcgccc gaggatcagc catagtgaac tgacgctttt
     4261 cacccggcag ttggcaacct taagcgcagc ggcattaccc ctggaagaga gccttgccgt
     4321 aattggtcaa caaagcagta ataaacgact ggctgacgtg ttaaatcagg tacgcagcgc
     4381 catccttgaa gggcatcccc tttcagatgc attacagcat ttccccacgc ttttcgattc
     4441 gctctaccgt accctggtaa aagcgggcga aaagagcggt ctgctggccc cggtgttgga
     4501 aaagctggct gattacaatg aaaaccggca gaaaatccgc agcaagctca ttcagtcact
     4561 gatctacccc tgcatgctca ctacggtggc gattgtggtc gtgattattc tcctcactgc
     4621 tgtcgtgccc aaaattaccg aacagttcgt gcatatgaag cagcaactgc cactgagtac
     4681 acgcattctt ttaggtctga gcgacacgtt gcaacgtacc ggcccgacat tattagcgac
     4741 agtatttatt gtcgctgtgg gtttctggct ctggttaaaa cgcggcaata accgccaccg
     4801 ttttcatgcc atgttactgc gcgttgcgct catcggcccg ctgatttgcg ccattaacag
     4861 cgcacgctat ctccgcactt taagtatttt gcaatccagc ggcgtccctc tgctggatgg
     4921 gatgaatttg tccaccgaaa gcctcaacaa cctcgaaatt cgccagcgcc tggcaaatgc
     4981 ggcagaaaac gttcgccagg gtaacagcat tcatctttca ctggaacaaa ccgcaatttt
     5041 cccgccgatg atgctctaca tggtggcctc tggcgaaaaa agcgggcaac tcggcacgtt
     5101 aatggtcaga gccgcagata accaggagac actccaacaa aatcggatcg ccttaacgct
     5161 ctccatcttc gagccagcac tcattattac gatggcactg atcgtcctgt ttattgtcgt
     5221 gtcggtactc caacctcttc ttcaacttaa ctcaatgatt aattaaggaa atcatatgcg
     5281 cgcaacggat aagcaacgcg gttttacatt actggaaatt atggtggtaa ttgtcatcat
     5341 tggcgtactc gccagcctgg tggttcccaa cctgatgggc aataaagaaa aggcggataa
     5401 acaaaaagcc gtcagcgata ttgtggcgct ggaaaacgcc ctggatatgt acaagctcga
     5461 caatcatcgc tatccaacca caaatcaggg gcttgaatcc ttagtagaag cgccgacact
     5521 gccaccgctg gccgcaaact ataacaagga aggttatatc aagcgtctgc ctgccgatcc
     5581 ctggggcaat gattatgtcc tcgttaatcc tggtgaacat ggtgcatacg atctgctttc
     5641 agcaggaccc gatggtgaaa tgggaaccga ggacgacatc accaactggg gtttgagcaa
     5701 gaagaaaaag taagtcagcg atgaatcagc aacgcgggtt taccctgctg gagatgatgc
     5761 tggtgctggc gctggtcgct atcacggcaa gtgtggtgct cttcacatat ggtcgggagg
     5821 atgccgccag cactcgtgcg cgcgaaacag cggcgcggtt taccgctgca cttgaattag
     5881 ccatcgaccg cgccacgctc agtggtcagc ctgtcggtat ccatttctct gattcagcat
     5941 ggcgcattat ggtaccgggc aaaacaccat cagcctggcg ctgggttcca ttacaagaag
     6001 atgccgcaga cgagagcaaa aatgactggg gtgaagaact ctcaattcaa cttcagccat
     6061 ttaaacctga tgacagcaac caaccgcagg tggtgattct ggccgacgga caaatcacgc
     6121 ccttctcttt gttgatggct aataccggga ccggtgagcc gctcctgacg ttagtcagtt
     6181 caggctcatg gcccctggat caaacgctcg ccagagatac ccgcccatga acaaacaatc
     6241 agggatgaca ctgcttgaag tcttactggc gatgagtatt ttcaccgctg ttgcgctgac
     6301 tttaatgagc agtatgcaag ggcaacgaac tgccatcgag cggatgcgta acgagacgct
     6361 ggcattgtgg atagcggata accagcttca gtcacaagat tcattcgacg aggaaaacac
     6421 gtccagttct ggcaaagagt taataaatgg cgaagagtgg aactggcgca gcgatatcca
     6481 ctcaagcaaa gacggcacgc ttctggaacg caccataacg gtgacattac ccagcggcca
     6541 gacaacctcg cttacgcgct atcaaagcat caataataaa tccgggcagg cacaagatga
     6601 ttaatcgcca gcagggtttc acattgctgg aagtcatggc ggcgctggcg attttctcaa
     6661 tgcttagcgt gctggcgttt atgatttttt ctcaagcttc cgagttgcat caacgttcac
     6721 agaaagaaat ccagcagttc aatcagctac agcgcaccat caccattctt gataacgatc
     6781 tcctgcaact ggtcgcccgg cggaatcgga gtacggacaa aatcatggtg ttgggtgaag
     6841 aagcgatttt taccacgcaa agtcgcgatc cgctggcccc tctcagtgaa gcacaaacct
     6901 tactcactgt tcactggtat ctgcgaaatc atacgctcta ccgtgctgtt cgtacttctg
     6961 tggatggcag gaaggatcaa cccgcccagg cgatgctgga acatgtcgag agctttcttc
     7021 tggaaagtaa cagcggagaa agccaggagc ttcctctctc agtcacgtta catctgaaga
     7081 ctcagcaata tggggcgtta cagcgccgtt ttgcattacc tgagcagtta gcgagagaag
     7141 agtcgccagc tcagacgcag gccgggaata ataaccatga ataatgaaca acgcggcgta
     7201 gcactgctca ttgtactcat gttactggcg ctcatggctg ctctggctgc agacatgacg
     7261 atcagttttc acggccagct tcatcgaacc cgccaggtaa accatcattt gcaacggcaa
     7321 tatgatattg agctggcgga gaaacttgcg cttgccagcc tcacccagga cgttaaagat
     7381 aacgacagac aaaccacgtt gcagcagtac tgggcacaac cgcaacagtt gcagctggaa
     7441 aatggcaata cagtgaagtg gcagttacgc gatgcccagc actgttttaa tctcaatgcc
     7501 ttagcaaaaa tctctgacgc cccgctggct tcccctgatt tccccgccca ggtattcagt
     7561 gccctgctga ttaacgccgg tatcgatcgc ggaaacaccg atgagatcgt gcaatcaatc
     7621 gctgactata ttgatgccga tgattcgcca cgctttcacg gcgcagaaga taacttttac
     7681 caaagccaaa cgccgcctcg ccatagcgcc aatcaaatgc tttttctgac cggtgaattg
     7741 cgtcagataa aaggcataac cgaaaacatc tatcagcggc ttatcccgta tgtctgcgtt
     7801 ctcccgacct cggaacttag catcaatctt aatatgctaa cggagaacga tattccgctc
     7861 ttcagagcat tattcctcaa taacatcacg gatgctgacg ctcgggtctt attacaaaaa
     7921 aggcccaggg aaggctggct aaccacagat gcatttcttt actgggcgca gcaggatttc
     7981 tccggcgtga agcccctggt tgctcaggtg aaagggcatc tctttcccta tagccgctat
     8041 tttacgctga gtacggaaag tatcagcgac gaacaatccc agggatggca aagccatatt
     8101 tttttcaacc gtaaacagca gagcgcccaa atctatcggc gtacgctgca actctactaa
     8161 tcataaggat cgtgatgcct gaatcgttga tggtaatacg ttctttctcg acgctgcgta
     8221 agcattggga atggatgacg ttctccgcag atagcgtatc ttcggtacat acgctcacag
     8281 acgatcttcc gctggggtcg ctggccgatc agcccggtgc cggaaatgtt caccttttga
     8341 tcccacctga gggtctttta tatcgttccc tcacgctgcc caacgccaaa tacaaactca
     8401 ccgcgcaaac attacagtgg ttggcggagg agacattacc cgacgccagc caaaattggc
     8461 actggacggt tgtcgataaa caaaacgaaa gtgtcgaggt gataggcatt cagtcggaga
     8521 aactcagtcg ttatcttgag cgtttacata ctgccggact caacgtgacg cgggttttgc
     8581 cagatggctg ctatcttccc tgggaagtag atagctggac tctggttaac cagcaaacaa
     8641 gctggcttat tcgcagtgcc gcacatgcct tcaacgaact ggatgaacac tggttacagc
     8701 atcttgctaa ccagtttccg cctgagaata tgcgttgtta tggtgtagcg cctcacggcg
     8761 ttgcagcggc caatccactt atccagcatc ctgaaatacc atcactgagt ttgtattctg
     8821 ccgatattgc attccagcgc tatgacatgc tgcatggtgt ttttcgtaaa cagaaaactg
     8881 taggcaaatc gggaaaatgg ctcacccgcc tggcagtaag ctgtctcgtt ctcgccattc
     8941 tttcttttgt tggcagccgg ggcatcgcgc tctggcaaac gcttaagatt gaagatcaac
     9001 ttcagcaaca acagcaagaa acctggcaac gttatttccc gcagatcaaa cacacccaca
     9061 actttcgttt ttactttaag cagcaactcg ctcagcaata tcctgaagct gtaccgttgc
     9121 tctatcattt acaaacactt ctgcttgaac actctgaact gcagttaatg gaggcgaact
     9181 atagccagag gcaaaagtcg ctgaccttaa aaatgagtgc taagagtgaa gccaatatcg
     9241 atcgtttctg tgagttaaca cagtcctggc ttccgatgga aaaaacagaa aaagatccgg
     9301 tcagtggtgt atggacagta aggaactcag ggaaatgatt aaatcatggt gggcagaaaa
     9361 atccacatcg gaaaaacaga tcattgcggc actggcggtt ctctcgctcg gcgttttttg
     9421 ttggcttgcg gtgataaaac cgatagatac ctacattgaa gagcatcagt cccacgcgca
     9481 aaaaattaag aaggacatca aatggatgca ggatcaagcg agcacccatg gtttactcgg
     9541 tcatccagcg cttacgcagc ccatcaaaaa tatccttctc gaagaagcaa aacgtgagaa
     9601 tttgactatc acgctggaaa acggtccgga caatactctg acaattaatc ctgtcacagc
     9661 tcccctcgaa aacgtctccc gctggctcac tacggcacag ataacatacg gtattgttat
     9721 tgaagacctt cagtttactc ttgccggaaa cgaagagatc accctcaagc atttgtcctt
     9781 cagggagcaa caataatgac aatgttactg ccgctcttca ttctggttgg ttttattgca
     9841 ggttattttg ttaatgccat ggcctatcac ctctcgcctt tggaggataa aacggcgtta
     9901 acgtttcgcc aggtattggt tcatttctgg caaaaaaaat atgcctggca tgatacagtg
     9961 cccctgatac tttgcgttgc tgctgctatc gcctgcgccc tggcaccttt cacgcccatc
    10021 gtgactggcg cactctttct ctatttctgt ttcgcactca cgctcagtgt tattgatttt
    10081 cgcactcagc tcctgcccga caaactcacc ttaccgctgc tctggcttgg tatttaatgc
    10141 gcagtctgga ttaattgatt tacatgatgc ggtttacggc gcggtcgctg gctatggggt
    10201 gctgtggtgt gtttactggg gcgtctggtt agtttgtcac aaagagggat tgggctacgg
    10261 tgatttcaag ctactggccg ctgcaggcgc gtggtgtggc tggcaaacgt tgccaatgat
    10321 actgctgatt gcctcgctgg gtggcattgg ttacgccatc gtttcacaac ttctgcaacg
    10381 ccggactata accactatcg cattcggccc gtggcttgcg ctcggcagca tgataaacct
    10441 gggatatctg gcctggatct cttattaacc gggagggttc tccctcccga cacggctcaa
    10501 ccttcttcgc gaatctgtgc ttgcagataa ttttgcagtc ccatcttctg aatcagatca
    10561 agttccgttt ccagccagtc gatatggcct tcttcatcgc gcaaaatttc tatcatcata
    10621 tcgcggctga cgtaatcatg aacgctatcg gcataaccaa ttgcctcacg caaattcttc
    10681 gcgccatcca gctcaagtgc cagatcagaa cgcagcattt cctcaacatc ttcaccaatg
    10741 ttcagtttgc ccaggtcctg taagtttgga agaccttcca gaaaaagaat gcgctctata
    10801 taacgatcgg cgtgtttcat ctcatcaatg gattcatgat actccacatc attgagacgt
    10861 ttgagacccc agtttttaaa catccgggca tgaagaaagt actgattgat tgcgacaagc
    10921 tcatttccca acagtttgtt gagataattt ataactttag tatcaccttt cattttttga
    10981 ctccttcgct tccactcttt gaagcgtaga acggcttacg agtaagtcaa aaaacacact
    11041 ccgtttatgc ggactcctta aactccggca gttgcattaa ttcatcctcc atcacttcac
    11101 gcgcggcccg gacacattta ccgcactgat tgccgaccgg aatgaatttt tttaattgct
    11161 ggaacgagtg gggggaaaac tggcgcacag cctgacgaat ttttttatcg ctgataccat
    11221 tacaaagaca aacgtacata gagactcccg tatactttct tcaaaagtaa atgaaaatag
    11281 ttcttatttc aattgcagga agactctttt ttaagcaaag aatagaaata tgaataaaaa
    11341 taatgggcta ccaaaagtag cccattgaca aaaaatgcgg cgataccgga aggtatcgcc
    11401 aacacattta ttgcttagta aacggcgcga agatttcgct aaatgcgtac ttcggagcct
    11461 gttctttagt cagaccgtgg aattccggag acaggttagt gccgcccggc aggtcgcgcg
    11521 cgattgacca gataccaacc ataccgagat tacgcttctg cgcatcctgc atgaccagac
    11581 gagcatcaga gagatagaac acctcaccct gaacgtcgtt cacgccaacc atcggcgtgg
    11641 tacccatcat ggcgtcaatt tctgcatcac tcttattgcc atagaggcct ttcagctgcg
    11701 catgcaggtt ggcaatcgca gacgttgcac acttaccgtg gatgttttgg ccttcggtgt
    11761 ttgcagactg acaaatagcg ttaccgtagt ccattgtcat cacgttcaca cccgccagct
    11821 caacaccttt cgctttggca tcgctcagga cattcatccc ttccggcgtc aggcctgtcg
    11881 gcagaattgg caaggtatac cagatagcaa tatctttgcc ttctgacttc catttatcct
    11941 gcactttctt cacagcaagg ttacgacgtt caatagatgc ctgatccgca acccaggtgc
    12001 cttcgatatc gaagtccagg actctgaggt tcaggttatc aacgatgtca taataatgct
    12061 gcattagatc gtcaacgttc ttacaggaag ctgccagcgg ggcattatta gcaccaccga
    12121 tagacagcat cacatcaccg ccagcctcac gcagcgcttt gattttgctg tactgagcgt
    12181 aattctgcat accgtaagcg gtcccccatg tcggcagaca ggtgttcgca tctttactca
    12241 ccacaaacgc cagcgtgaag tggttgacgt tatgattctt agccagcgca gccagatccg
    12301 ggatcgtatt cagcgtaaag tcgacatacg gtgcataaac gtgtttcggc cacgctttct
    12361 tcggcgtacc tacgcgctct ttggttccgg tccagtcaac aaagacacgc cacgggttgc
    12421 tgtcagaagg agaaactttc tgcactttac tctgagaaat gtagttctca ccgttaaagc
    12481 gaatcagcgt atcactggca taaagcgttt ctggattaaa ctgcggcgca ttattcagct
    12541 cttcgttgct atagctctga gtcttaccta acggcttcca cgggcgacta ttgctaccgg
    12601 tggcgttttg gttcgcaacc agagcagggt tgtcactttg cgtccagaac agcgcttcat
    12661 aagcctgccc atcaacaaca acgcgatcac ctttcacgta cacggtggaa gcggaccatg
    12721 ctttcgccgg ttcgtatttc ttccacgggt tatcgccgcc ggggacaaag ccccatgcgt
    12781 cttgatcgac agaggtttta taatgtgcat cgttataatc tacgatgctg ttcgcgctat
    12841 acgctttgct ggcctggaaa ccatccacaa taatgacgcc gccattatca atttcacagg
    12901 tagtcgggtt accgtactga ctcatttccg cagcggtagc tgtacgcacg agacgccatg
    12961 ggttattgga gttttctaca ctggtgccac gcgggcaatc ttcagaacca acccaccagg
    13021 cattctgata aacattacca ttgaagataa catggtaaat ttcgctgcct tcctggccac
    13081 tccacgcctg cagtgagtaa tcgttactgc tatccgctgg tgttgatggc tcgttgttat
    13141 ccggagttgg agtaactgga gtttccggtg tttccggagt tggatcaacc ggagtcacag
    13201 caccgccatt attatccggc ttaacggaac aggagcctgg gttaccatac tgagtgattt
    13261 ctgttgccgt tgccgcgcga acataacgcc atgggttgct ggcatcgttt tctttcgcat
    13321 cgcctgggca atttgccgcg cctacccacc aggcgttctt ataaatgctg ccgttgtaga
    13381 taacgtacca tgtagaagaa ccttcctgac ctttccaggc tacaacagca tagttgtcgt
    13441 ttgacttatc agctggggct ggctgaacat caggggccgg tttaacgtcc ggtgccggtt
    13501 tcacatccgg tgcaggctgt ggtgctgaag tacaagactg tggattactg gtttctgaga
    13561 tttccgttgc cgtagcggca cgaacataac gccatgggtt gctggcatca ttgcctttcg
    13621 catcacccgg gcagttagaa gaggctaccc accaggcatt tttatatacc gcaccattaa
    13681 agacgacata ccaggtttga ccgccctgct gtttattcca ggcaactaca gaagaattag
    13741 acggaacagt gccctgtgct ggtgtagccg aaccgccatt agcgggcgta ttagatgctg
    13801 gcgtatttga agttgaagaa gatgagcttc cgctcttttc gcaggaaagt gtattgccaa
    13861 actgactaat ttcagcagct gttgcggtac gctttaaacg ccacgggtta gttgcatcat
    13921 ttgctttcgc ttttcccggg caatttgtag aagaaaccca ccaggcattt tcataaattt
    13981 tgccatcgaa aataacctga tatttattac caccttgttg attattccat gcttccattg
    14041 ccaatgctgg cagagag
//
LOCUS       DACADA010000120        13673 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.122, whole genome shotgun sequence.
ACCESSION   DACADA010000120 DACADA010000000
VERSION     DACADA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.122"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(149..838)
                     /locus_tag="ISB17_003477"
     CDS             complement(149..838)
                     /locus_tag="ISB17_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1981368.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(949..1653)
                     /locus_tag="ISB17_003478"
     CDS             complement(949..1653)
                     /locus_tag="ISB17_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1981369.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(1640..2476)
                     /locus_tag="ISB17_003479"
     CDS             complement(1640..2476)
                     /locus_tag="ISB17_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1981370.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(2469..3302)
                     /locus_tag="ISB17_003480"
     CDS             complement(2469..3302)
                     /locus_tag="ISB17_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1981371.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(3302..4351)
                     /locus_tag="ISB17_003481"
     CDS             complement(3302..4351)
                     /locus_tag="ISB17_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1981372.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(4451..6019)
                     /locus_tag="ISB17_003482"
     CDS             complement(4451..6019)
                     /locus_tag="ISB17_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1981373.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(6394..8541)
                     /locus_tag="ISB17_003483"
     CDS             complement(6394..8541)
                     /locus_tag="ISB17_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8122..8124),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HAP1981374.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(8739..10205)
                     /gene="mdtP"
                     /locus_tag="ISB17_003484"
     CDS             complement(8739..10205)
                     /gene="mdtP"
                     /locus_tag="ISB17_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAP1981375.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLNGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAISAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(10202..12253)
                     /gene="mdtO"
                     /locus_tag="ISB17_003485"
     CDS             complement(10202..12253)
                     /gene="mdtO"
                     /locus_tag="ISB17_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAP1981376.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            complement(12253..13284)
                     /gene="mdtN"
                     /locus_tag="ISB17_003486"
     CDS             complement(12253..13284)
                     /gene="mdtN"
                     /locus_tag="ISB17_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAP1981377.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVSDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(13303..13578)
                     /locus_tag="ISB17_003487"
     CDS             complement(13303..13578)
                     /locus_tag="ISB17_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981378.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         3260 a         3622 c         3576 g         3215 t
ORIGIN      
        1 catcaatgtc tgatgcgatg ctgacgtgtc ttatcaggcc tacgtcagac agcgcaatag
       61 cctgatttgg cgtgattttt gtaggtcgga taaggcgttt acgccgcatc cgacatcaat
      121 gcctgatgcg acgtttgctg cgcctacctt aaccgttagt taatttttca aactcttcac
      181 aaccggtatc aaatccttcc atacagctca gattcaacca gtgcagcgcc ttttgtttat
      241 tcttctcgat aaaaccctct tcaccgttta agaacatcat tcccgcccag tattcggaat
      301 aaccggttcg ggaaatcgca gagctacgtt tgaaatacca ggttgctttg tcatcgtcgg
      361 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcag
      421 agtcgttctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt ggataatccg
      481 gtttacccgc ttgcgtattt accagaatat gcgccagcgt tacttcacct tctttactgc
      541 ccgcttgcgc tgctttctcg gcaagcactt ttgcttgtgg atagtccaga ctgaccggat
      601 tggtgatttt gatctgcgcc aacagcgcgc aggcatcggc atcaccgctg tcggcggctt
      661 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
      721 aatattgtgc gcgtcggtct ccggcctctg ctgcctttaa atactggctg ccaggctcgg
      781 agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa agtgataatt tttttcattt
      841 tgataatgaa gtttggtagg ggagagacag tataaagtgt tcgggtggac aaaaaaacaa
      901 ctgtaaaaag acctgaaagc agaatgaatt aatgcaatgt caggtttttt aatccatccg
      961 atgttccccc ttttccatgg cgtcgcgatc aaaaaatcgc tgaatttttc cctcggacat
     1021 caatgccgct cgatcagaca tatgcgcgat gacatcggca tcgtgactga ccagcagata
     1081 tgtcatttgg tgttccagct taagttgatt caacaaatta aggatttccg cttgtaccga
     1141 catatccaac gccgaggtgg gttcatccag cagaagaagt tgtggtcgta acaacagggc
     1201 gcgagcaata accacacgtt gccgttgacc accggaaagc tggtgtgggt agcgacgagc
     1261 ggcgtcgaaa ggcaagccaa cctgttccag tgcagtctgg acgcgttttt ctatttctct
     1321 ttcgccacga attttgagtg gttcagccag cgttcgccac agagtatgat tgggatgcaa
     1381 cgaggcatag ggatcctgaa acaccatttg cacattgcgg cggagatcgc cctgaaaacg
     1441 cattcctggt ttgatggtgt gcccaaacag tgacacctga ccgtgccagt cgcgctgtaa
     1501 tccagcaatt acgcgcaaaa tcgtggattt tccgcagcct gattcgccaa ttaaactaaa
     1561 tgtctctccg gcattaaccg aaaaactggc ggcagaaact gcggttttct cagcaaaagt
     1621 cacctgtaag tgattaattt caacaatcgt catggtattt ctccggcgtc attgccgtcc
     1681 tgtccagcgt cggcagcatc tgcccgtaag tttgtgcatt tgggcggcaa gtccacagtg
     1741 tgcgcgtgta aggatgcgtt gctgtaggta gcgcagcagc gtgcatttca tccaccttat
     1801 ttccctgata catcaccagc acccgatggc aaaattgggc aactaatggc aggtcgtgac
     1861 tgatcagcaa cattgccatc tgacgttgtt cacactgggt aaccagcagt tccagaatct
     1921 gattacgcag gcgcacatct agtgctgatg ttggctcatc ggcaatcaat acccggggat
     1981 cgttgatgag cgcaattgca atcatcactc gctgccccat gccgccagaa agttcacgag
     2041 gatagctttg cagtactcgc gtattgaggc cgacagcctg aattgcggtg tttattttct
     2101 cttctttctc gcggcggttc agacgttgat ggagcgtaag cgcctcttcc agttgcgctt
     2161 ttactgtctt tactggatta agcgcgtaac gcggatcttg caataccatc gcgatatcgt
     2221 taccgcgcaa ctgttgccat ccacgcgcgt taagggtgag tgcatcacgc cccagaatgt
     2281 taagtgtgtc cgcggttact acacccggtt tacgcaccag ccccatcagg gcgcgggcgg
     2341 tcatggactt acctgaaccc gactcaccga ccagcgccag acgttcattg cctaatgtaa
     2401 aactgacatt gttgacgacc cgggactccg gataatcgac gcagagattg ttgatactaa
     2461 gacgtgagtc agtcatgttg aggctccaga acatcgcgca agccatcacc cagcagatta
     2521 aaggccagac tggcaattaa gataattgcg ccgggagcgg cggcaatcca ccattgatca
     2581 aaaatgacct gcataccgtc ggcgatcatg gctccccatt ccgccattgg cgggcgagca
     2641 ccaagcccaa gaaaacccag accagcagcc gccagaatga tacccgctaa atccagcgcc
     2701 agacggacaa tggcagatgg caggcacaat ggcaaaatat gaccagccag cagacgcctg
     2761 ccgcggatgc ccatcatttc tgcggcggca agataatcac tgtgtcgcag acgttggatc
     2821 tcactccgtg cctgacgcgc ataagcaggc caggtggtta atgcaagggc cagtgcgccg
     2881 ttaaccagcc ccggccccag catggcaaca aaagcaaaag ccagaattaa acgcggcatt
     2941 gacattacta tgtcagtaaa acgcatcagc acgcgttcca gccagccgcc gtagtacccg
     3001 gctaaaatgc caatcagcag accgaccggg agtgtaatga ttgtcaccaa tgccaccagc
     3061 cccagtgccg ggcggctgcc gtagattaaa cgtgaaagta ggtctcgccc gtaactgtca
     3121 gtgccgagcc agtgctgacg actcggtgct tgtaatcgcg ccgccgcatc ctgccagtta
     3181 ggatcgtgag gtgccagcca cggcgcgaag agcgcaatta gcatcaataa caggataatt
     3241 atcaacccgc aaaacgcggc gggtgaatgg cgtaaacggc gtaaaaagag aaacatcggc
     3301 atcagcgtaa cctcggatcg gtcagccgga ccagcaggtc ggttaagtta tttatcaaca
     3361 caaagcagac gccaatcagc agcgttcctc ccatgattgc tgtggtatca cccgcgaaga
     3421 gcgctgtagt gaggtagcgc ccaatacctg gccatgaaaa gacggtttct gtcagtaccg
     3481 cgccttccag cattgatgta taggcgaggg cgataacggt gagcaaggtg ccgcgaatgt
     3541 tgggtaaaac atgacgtagc aaaattgtca tctcgctggc ccctttggcg cgcgccagca
     3601 gaatatattc tttattcatt tcgccaagac aggcggaacg agtcaggcgc gtaatactgg
     3661 cgagcgagaa gtacgccagt aataaaaccg gaagtatcaa atggttgatt gcattggtaa
     3721 atgcctcgcg atcgccagaa agccacgtat caatcagcgc aaatccgctg cggggttcta
     3781 tggtgaattg ataaatatca tccagcctgc ccggcccggc actccattgc agtctggcgt
     3841 agaacagcgc cagcatcagc agaccaagcc aaaaaacagg gacggaattt cccagtagag
     3901 ttaatgttcg cacggtgaaa tccagcggag aaccgacata acgggcgcaa agtacacccg
     3961 caatgacgcc gacaacagca ccgacaataa gcgccaacgt tgccagttca atggtcgccg
     4021 ggaatgctga tagcaagtcc tggagtactg gttgcccggt ggagctggcg atgcccagat
     4081 cgccgtgtgc cagattcatc aggtaatgcc agaactggac ggctaacggc tgatccaatc
     4141 ctaactggtg gcgtacctga tcataggtgg actggctggc gtgatcgcca acgatttgta
     4201 gtacgcgatc gaccggtgag agagccgaaa gcgcaaaggt aatcagcagc aggccgagta
     4261 gcgtcagagc aacggtaaac agcccttgca acagcgcgag cagagggcgt ttgcctcgcc
     4321 atcttgcgcg agtggaaaaa acgacagaca tgatctttcc atacagtgat aaatcagcgg
     4381 ctggcctgca atacaggcca gccctggtac cgcgcccggt aacatggctg gacgcgagtt
     4441 gcatacaggg ttatttggtt acctgatcaa accagaccat atcggcattc aacccttgtt
     4501 gatacccctg cacattatca cgcatgacaa tttgcgtttt gccttgatca ataaagacat
     4561 acggggaact acgttgtagt tcttgttgca ttttcttata caaatcaaga cgtttagtgc
     4621 tatcaggttc agcgatggcc gccagcgttt ctttgttcag ttccgggatt ttccagccgt
     4681 ttaacccggc aacggtgctg gatttgccat cgttataggc gaaagagctg gcgttggaat
     4741 gtgcgtcaaa ataatcaggc aaccacatac gaatggcccc ctgatgctgg tgggcgcgaa
     4801 cgcgagagta gacctgactc cctgcggctg gcagcaaatc aacttttaca ccgccctgag
     4861 caaagctggc ctgaattgac tgcgcgatgg taataaatgg cggtttattt tcgacatcaa
     4921 gcgtgaagtg cgggtctttg attccggctt tagcgaggat ctcttttgct ttggctggat
     4981 taaaggtaaa cggtgtttct tccagtgctc caggaaagcc cacgggcagg aaactctgat
     5041 gggtaaaata ttgtcctttt aagagatttt tggtgatgcc gtcgtaatcg accaaccagc
     5101 gagcggcctc ccagaacgca ggattactca tcagcgggtt atccttgtta ccggtattga
     5161 aggccaggta gttctgttcg gcggaggcaa tgctcatcac ttttacgcca ggtttaccct
     5221 gtaatgcatc gatttgatcg gctcccagat cacgagccat atcagcatca ccttgttgga
     5281 tcagcaggcg acgtgttgcc ggatcgggaa catttttgat gatgacgctt ttcagtttcg
     5341 gggcgccggt tggtgagctg gcgttggctt ccatcacgat ggcctgatgc ggttggtaag
     5401 tacgcatttt aaacgcgccg ctgccagcgg aatgcatctt cagccattca ttaccaaagt
     5461 cgttattttt ggcgtttggc gcaaccagct tctcgtcaac aattgaggcg attggtgtgg
     5521 agagaatatt cagtgccaga gccgggctga cgtcggcact ccattgcacc tggacggtat
     5581 ggtcatcgat tttcttcaac tggctggcga tattttccgg ctgccatccc agcacattga
     5641 gaataaacgc accggattta ttcagtgtga cagcccggac ataagagtaa atcacatctt
     5701 ccgggcgtac cggattacca gaggcaaatt tcgcatcgct tttcagtttt atggttagcg
     5761 ttttggctgc ggggtcagcc tgccaacttt ccgccagaat cggagtaatt ttcgtcggat
     5821 cattacggtc cggttgcact acgcgttgat acagacttgg tacagtctga atacttgaga
     5881 gttcattagc ttctgccggg tccaggctca caatatcatc cagcccctgg gctacaacca
     5941 acgtattagg cggtgtagcg gcaacggttg tggcggaaat tgtgcttaaa accagtaaag
     6001 gtaatagttt tttaatcata tgcttcccaa tccctgaaat tactttgagg ttattaatat
     6061 tctatcagac ggggttttgt tgcattaatc gcgccaaaat atcccgttaa tagaacgagt
     6121 taaacggtcg atgatttctc accttaccga aatttggttc atcaaatgta aagtattatt
     6181 acgttaggcc gcgatgagaa ttgagtaaag ataattctca tttgcaatcg tgactggaat
     6241 agaagaacaa ccgagaagac gtcgctaata accaggatag gctggtttga ctgcgcaggt
     6301 atgtataaaa aaatccggaa acagagagtc gtttccggat tgatacaaag gcgttgggta
     6361 atatcaccgc ttttaacgtg agtttaagcg gtattacgcc agtgccgctt cgcgcaggcg
     6421 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
     6481 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
     6541 ttcggtcacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
     6601 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
     6661 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
     6721 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
     6781 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtattcgt cggtgagttt
     6841 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
     6901 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
     6961 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
     7021 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
     7081 agtggcaatt tcactgatga tttgccagtc cgttttcaga tcccacttcg gctcaaccgc
     7141 cttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
     7201 cgacgttgac ggtaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
     7261 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagtt ccgcgtcagt
     7321 ttgcagcgga tcttcgccca taatgtacgc cgctctcact tcaccatgcg ccacgcggtg
     7381 cggcagctcg ctgatacgat aaccggtatg cgctggcagg ctctctacgc cccaggcttt
     7441 ggcaaatttc tcgcggttag ccggatcttt cacgtactga tagcccggat acgtatccgg
     7501 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
     7561 cgcatgtggc ttaccgaggt taccggtcag catcgcgagg ctggtcagag aacgcacggt
     7621 ttccacgccc tgatagaact gggttacccc catgccccac aggatggcgg cgctttctgc
     7681 ctgggcatac atccgcgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
     7741 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
     7801 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
     7861 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
     7921 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
     7981 gtgattcgcc acgattgggt gggaatccgc cgggttatac ccgaaaacga acactaaatc
     8041 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
     8101 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
     8161 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cgcgcgagga
     8221 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
     8281 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
     8341 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga agtcccagcc
     8401 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
     8461 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
     8521 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
     8581 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
     8641 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
     8701 acgaaatacc gcgtgacagc catcacgcgg tagacatttt attttttctc gacgacggga
     8761 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
     8821 ctgtccagca tcagtaatga catctcttcg gcaagcactg gcaaccgggc ttcggtggcc
     8881 tgtaagcggc tggttaagcc gcgttggtag gcggcctcgg ctgcgcgctg ggtgaagcgc
     8941 gttgcttcta cgcgttcagc ctgcatctct cgctcgtcgt tgagcgtttg cagacgtgtg
     9001 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
     9061 atgttgctgg cggcgcgagt gccttcgaga ttggcattca accgtccacc gtcaaacagc
     9121 ggcaatttca gacctgggat aaagttgaac tggcgactgg tttttttgaa taaggtatcc
     9181 aggtggatag aatccaggcc gaaaaacgct ttgatatcaa agctcggata gaacagcgcc
     9241 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
     9301 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa taccggtctg gacttgcggt
     9361 aatgccaccg gtttgatctc cggcatatcg ctggctcccg cgccaatcaa tgcacgcaga
     9421 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
     9481 atctgtgccc gcgcgccgtg gaaaggcact tgcgcttcca gaccgtgcgc cactttactc
     9541 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
     9601 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
     9661 actgctgcgg tttctgccag cgcggcatta tgcgcgctaa tggcggcagc aaccgctgag
     9721 cgatgcacac cccacaaatc gagatccagt ccggcaaaca aaccgactgt ggcttccgta
     9781 tagtacggcc cgtccatacc cagtgctggc gcatccatcg cataagggct taaaaagccg
     9841 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
     9901 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
     9961 gtgtgtgaac catttagagt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    10021 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    10081 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tacgtaccag ggcacagccg
    10141 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    10201 atcattgtgt ggctccttgt gcctgttcct tggcgggcgt taatgccggg gctgtccagt
    10261 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    10321 tggcctgcga ggcgggtgtt tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    10381 ccagaccagc ggcgtatttc tccagtgcgt cggcaaactg acctgctctg tccggctgta
    10441 gtgcgttatc cagcgcctgc gccgagttcc aggtggcatc ccaggcgtga agaatatcgc
    10501 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    10561 gttgacgctc cagcaccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    10621 gacctatgcg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    10681 gcattacttt actcaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    10741 cgctttcagg ccagacaaag gtgtaaatca ccgccgacac cacggtacca atgatgatac
    10801 ccagagcgcg atcgcgaatt tccaccaggt cgtacaccgg gccaaaaacg ttttcgagcg
    10861 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    10921 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    10981 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    11041 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagcgacga
    11101 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg tagaaggtgt
    11161 aacagatcaa acaggcgagc agcgttttta ccgcgtagcg catatagtct ggattggtaa
    11221 aagcgtcggc gactattgat ggcggtttgg tggcgggcgt tggcggcgta ttcgggtcca
    11281 tctgacccag ttgcaacaat gtctggcaga tattctccag gttacattcc cgtgccgcca
    11341 tcgcttcact ttcagtgatc cgccagtcgc tttgccagca ctgaccttcg gcaatggcat
    11401 gctgcagttt gttgatttct gaggctaatt tttgtcggaa ttcaataatt gcctgagaat
    11461 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    11521 cgcagctttg ccaccatgcg ctctgagttc gccagttggc atcgtccgcg aggcaaaaga
    11581 cattgagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    11641 ggctgtccgt caggtggcta atggcatcat caagccgatc attaagcgcc tgatgcatct
    11701 gcgtgatagc gcggctggga aaccacagca cgccgattaa cgtcatcaat aacgtcgggt
    11761 agaggccgac aacgatacac cacagggtta agcgcacgac cacttccgga tagtcgagca
    11821 tggcggggaa ggtttgcccg taaatagcga caatggcgac ggcgaaaaag accagcccca
    11881 gccgatgggt gcgcatcaaa aacatgcagc ccatcaggat cggcccggcg atgatcaatc
    11941 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    12001 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    12061 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    12121 gaatcaccag taggcagcct acccagagtt gcacggtctg cggcacacga cctggccgcc
    12181 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat ggcagggagt
    12241 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    12301 ttccgggtca ggtttatcaa ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    12361 gttaatagaa cgcgacactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    12421 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cgctcatcag
    12481 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcagtttcgc ggaaattggc
    12541 gatcacgtac cagtgacgag tgtcgattag ggtgaaaata gggcgcatgg cagaagcaaa
    12601 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    12661 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgggcgac
    12721 cagcgcatcg acgccgctga cggcactggc ggctgactgc gcctgcaaca atacggcatt
    12781 aaggtccgct tctgccgcac gctgggcggt tcttgcacgg tcaacctctt ccgcagaaac
    12841 aaaaccttct cgcagtagcg gttcggtacg gcgtaacgta tcgctggctt gtttcgcggc
    12901 ggcacgggct ttttctaccg tggcattaac cgagtcggca ccaaactgtt gcgcgtcaac
    12961 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    13021 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttcaccgc
    13081 ctggttgtcg ctgaccgcca gttctacaat gcgaccgctg acttccggca ccacatcaat
    13141 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    13201 gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt tactgcgagg
    13261 agcttttttc ggcgtacttt ccatgagcgc gtctcaaatt gattagaaaa atgccagcca
    13321 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    13381 gatatttgtc cgctgaataa ttcgcctcgt gatgagcgtc aaaataaggc tggcaatggc
    13441 gcagaaaaac cagccgggat aataagcgcc gatcaccggg attgccggag ataatgagca
    13501 tcccgaaacc atgaagacgg gaattatcca ggctattttt ttgagttgca tcgctatgcg
    13561 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    13621 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aat
//
LOCUS       DACADA010000121        13529 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.123, whole genome shotgun sequence.
ACCESSION   DACADA010000121 DACADA010000000
VERSION     DACADA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.123"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..669
                     /locus_tag="ISB17_003488"
     CDS             <1..669
                     /locus_tag="ISB17_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP1981379.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GGGK"
     gene            666..992
                     /locus_tag="ISB17_003489"
     CDS             666..992
                     /locus_tag="ISB17_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP1981380.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..1352
                     /locus_tag="ISB17_003490"
     CDS             1002..1352
                     /locus_tag="ISB17_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP1981381.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1349..1795
                     /locus_tag="ISB17_003491"
     CDS             1349..1795
                     /locus_tag="ISB17_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP1981382.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRACANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1792..2136
                     /locus_tag="ISB17_003492"
     CDS             1792..2136
                     /locus_tag="ISB17_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAP1981383.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            2203..2919
                     /locus_tag="ISB17_003493"
     CDS             2203..2919
                     /locus_tag="ISB17_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1981384.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            2934..3308
                     /locus_tag="ISB17_003494"
     CDS             2934..3308
                     /locus_tag="ISB17_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1981385.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            3404..3613
                     /locus_tag="ISB17_003495"
     CDS             3404..3613
                     /locus_tag="ISB17_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAP1981386.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            3661..6903
                     /locus_tag="ISB17_003496"
     CDS             3661..6903
                     /locus_tag="ISB17_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1981387.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVARVQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6896..7237
                     /locus_tag="ISB17_003497"
     CDS             6896..7237
                     /locus_tag="ISB17_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1981388.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNLTVRVVCREWSVTDNARHS
                     DFSCTIEQVVN"
     gene            7237..7935
                     /locus_tag="ISB17_003498"
     CDS             7237..7935
                     /locus_tag="ISB17_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP1981389.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIDGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            7946..8689
                     /locus_tag="ISB17_003499"
     CDS             7946..8689
                     /locus_tag="ISB17_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1981390.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTSEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            8635..9267
                     /locus_tag="ISB17_003500"
     CDS             8635..9267
                     /locus_tag="ISB17_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP1981391.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWY
                     QIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVIGRQG"
     gene            9610..13083
                     /locus_tag="ISB17_003501"
     CDS             9610..13083
                     /locus_tag="ISB17_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP1981392.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEI
                     TQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     AGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGITIS"
     gene            13151..>13529
                     /locus_tag="ISB17_003502"
     CDS             13151..>13529
                     /locus_tag="ISB17_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP1981393.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT         3061 a         3307 c         4396 g         2765 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 ggaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaacgct gccggagaaa
     1081 tgacggaaga atgggtgtca tgcgggaaaa ttcatgcgga tatccgaggc aggagcagcc
     1141 gggagcggat gcagtccggt gcggaaatgg cgcaggcgga aatccgcatc tgggtgcgcg
     1201 gtcagtccgg tcgggaaatc acggcagcgt cacgacttca tgtgctgagt ggtccatggc
     1261 gtgaccggat cctgaacgtt gtcgggctgc ccgtgccgga tgcgaccggc gggcgtctgg
     1321 aaattctctg tcggctggga ggggaaaaat gatcgaaacc ctgctggatt tttcggggct
     1381 ggaggacatc agccgcgatt tgcagcttct gagtggtgcg gaaaataacc gggtgctgcg
     1441 tgaggcaacc cgtgcgtgtg cgaatgtgct gaaagaagaa gtggtgtcac gggcaccggt
     1501 acgcagggga aaactgcgcc gcaatgtggt ggtcctttcc cggcgctccc gcgatggcgg
     1561 gatggaatcc ggtgtccata tccgtggtgt taatccggac accggtaaca gcgataacac
     1621 catgaaggcg gataacccgc gcaatgcttt ctactggcgg tttgtggaaa tggggaccgt
     1681 gaatatgcca ccgcacccgt ttgtgcgccc ggcgtttgat gtgcgcagtg aacaggcagc
     1741 tcaggtggcg attgcgcgga tgaaccgggc cattgatgag gtactgagac gatgacggag
     1801 gcggatttgt atcctcatct ggcgcatctt gccggcgggc aggtgtaccc gtatgtggtc
     1861 cccctgctgg atggcaggcc gtcggtggcg cttccgtggg tggttttcag cctgatttca
     1921 tcggtgtctg cggacgtgat gggcgggcag gcggagtcct cagtgtcggt gcagatagac
     1981 gtttatgccg ggactgtgac gcaggcgcgt cagatacgtc aggacgcccg tgaagccata
     2041 atgctgctgg ccccgggatc cgtcagtgaa atgcaggact atattccgga aaaccgctgt
     2101 taccgtgcaa ccctggagtt tcaggtcacg gtgtgacttt ttcttttttt ctacaaaacc
     2161 cataccccgc cgcgtgcggg ttttttatta tcaggaggca gaatgtctgc tttgtatgaa
     2221 cgctcacagc tgacgcaggt gatgatttca tctgccccgg cgactgctga aactatggat
     2281 aaggcggaat atctgcgcct ggactgcacc atcaaggaag tccagttcac cgctggtcag
     2341 aaacaggata ttgatgtgac cacgctctgc tccaccgagc aggagaacat caacggtctg
     2401 ggggcgtcgt ctgagatttc catgtcgggt aatttttatc tgaatcaggc ccagaacgcc
     2461 ctgcgtgatg cctatgacaa tgacgcgttg tatgcgttta aggtgctgtt tccgtccggt
     2521 aagggcttta agttcctggc ggaagtgcgt cagcacacct ggtcatccgg taccaacggc
     2581 gtggtggcag caacgttttc actgcgtatg aaaggcaaac cggtgtcctt tgtggtaccg
     2641 ctggcgtttg tgaaaaatct ggataaaaca cttaccgtga atacaggtgc gctgctgaca
     2701 atgtcagtca gtgccaacgg gggaacgccg ccgtataaat acgcctggaa gaaggatggt
     2761 cagccggttg acgggcagac gacagacacc ttcagtaagc caggtgcgca gtccgctgat
     2821 gcggggaaat atacctgcgt ggtgaccgat tcggcagaga aagcacagag tgtgacgtct
     2881 gttgaatgca ccgtgacagt gagcgcagcc gccggataag gggatgggtc atcatgaaaa
     2941 aggatctgaa aacgctggcg ctggccagac tgtcagggtt tcgtcataaa acggtgaagg
     3001 tgccggaatg gggtaatgtc agcgtggtgc tgcgggagcc ttcggcagag gcctggtatc
     3061 tgtggcagga agtgctcaat ggtgatggag aggatgacga taccctgtcg gtggtggcga
     3121 aaacccgccg taacctagaa gcggatgtga cgctgttctg cgatgtcctg tgtgatacgg
     3181 atctgcagcg ggtgttcgct ccggacgacc gtgagcaggt gctggccgtc tatggtccgg
     3241 tacatgcccg gttgctgcgt caggcactgg aactgatcgc tgatgcagag tcggccagaa
     3301 aaaagtagcc cgcccggaaa ttcgctttct gatgcgactt gcgctccgtc tggggcgcac
     3361 cttatccgaa ctgcggcaca gcctgagtgt gagcgaggcg atgatgtgga tggagttcga
     3421 cagggtatcc ccgctgggtg atgagcgcgg ggatatccgt aatgcacaga tcgtgaaagc
     3481 ggtttttggg gcacagggga tgaatgttgc actgaaggac gccatgctct gctggggcga
     3541 ggatgaggat aagccggagg tggatccgtt tgcggcgctg gaagacgcgc tgagcttcgc
     3601 agcacagtca tgaatgatga gaaccgctga ggcggttttt ttacgcccgg agaaaggtga
     3661 atggcgacgt tacgtgaact gattatcaaa atttcggcaa attcgcagtc attccagtcg
     3721 gagatccagc gggcttcccg catgggcagt gaatattacc ggaccctgca gaatggcggg
     3781 cgtcaggctg ccgcagtcgc ccgggtgcag cgacgcgccc tggctgagct gaacagccag
     3841 ttgacggaaa tccgcgcttc agctgccgga acggcggggg catttgcagg tgcctttgcc
     3901 accggacacc tgatttctct ggccgatgaa tggagttccg tgaatgcccg actgaaacag
     3961 gcgtcgcagt catccgatga attttcgtca tcacagaaag tgctgatgga tatcagccag
     4021 cggacgggca cggcattttc agataatgcg gccctgtttg cccgctcggc agcctcaatg
     4081 cgtgaatatg gttacagtgc tgatgatgtg ctgaaggtga cggaggccat ttccacgggg
     4141 ctgaaaatct ccggtgccag tacggcagag gcgggctcgg tgatcaccca gttcagccag
     4201 gcgctggcac agggtgtgtt acgcggtgag gaatttaatt cggtcaatga aagcggagac
     4261 cggatcgtac gtgcactggc tgcgggtatg ggcgtggccc gtaaagacct taaggcgatg
     4321 gcggacgacg gcaaactgac ggcggataaa gtcgttcctg cgttaatcag ccagctgggg
     4381 gtattgcgtg atgaatatgc cgccatgccg gaaacggtct ctgacgggat cacaaaggtg
     4441 gaaaacgcct ttatggcctg ggtgggtggc gcgaatgaag ccagcggagc gacgaaaacg
     4501 ctctccggcg tgctgaacgg tgttgccggt aatattgata atgtggcaac agccgcgggg
     4561 gcgctggttg ccgtcggggt tgcccggtac tttggcaata tggcctccgg agcggtgtct
     4621 gccacggcag gacttgtgac ggcagcacgt aatgaagtgg cactggcgga agcacagctc
     4681 agggggacgc agattgccac ggcgcgggca agggcagccg tgtaccgtgc acagcaggct
     4741 gtggcggcag cccgcgggac ggagatgcag attgctgcag aagcccgtct ggcggccaca
     4801 caggaacgcc tgaacagaaa tattgctgcc agaaccgcag cccagaatga gctgaacagt
     4861 acaacggcgg tgggctcacg tctgatgact ggtgcgttgg gactggttgg tggcgtaccc
     4921 ggactggtga tgctgggggc agcagcatgg tatacgctgt accagaatca ggagcaggcc
     4981 agggagtcag cgcgccagta tgcactgacg atagatgaaa tcgcgcataa aacgccgtca
     5041 atgtctttgc ctgaagcctc agataatgaa ggacgaacac gggcggcgct ggcagagcag
     5101 aaccggctga ttgatgaaca ggccagccgg gtgaaatccc tgcaggaaaa aatcgctgga
     5161 tatcagtatg ttctggctaa ccctggctgg acaaccggtg acggattcat gataaaccat
     5221 ctgacatcgg tgaagaccgt gacggaaggg ctttctcagg caacagagca gcttgccgtt
     5281 gagcagtccc gtctggcaca gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc
     5341 gggctggaag accgtcgtgt ggcgttaatt cgtcagcagg cggcagagca gaataaggtg
     5401 taccagtcca tgctggttat gaacggtcag catacggaat tcaaccgtct gctggggctg
     5461 gggaatgaac tgcttcagca gcgtcaggga ctggtgaatg taccgttacg gctgccgcag
     5521 gccaccctgg atgataaaca gcagagcgcc ctgacaaaaa cagaacgtga gctggccctg
     5581 tcccgactga agggggagga aaaagagcgt gcccggctgg ggtatgcggc ggatgacctt
     5641 ggttttgtgg gggattcgta tcaggaggcg agacagcgtt atatcagtaa tgctctggaa
     5701 gcctggcgta ataacgaggc gaacaaacct aaatcccggg gtggaaaatc agagacggaa
     5761 aaagcggaag acagtttttc ccggctactg aagcagcaga aagagcaact ggcactggcg
     5821 ggtcagaata cagagctggc gaagctgaag taccagactg cgcagggcga actgaaaacc
     5881 ctgacggaga tgcagaagca ggaactgctg cgtaacgcgg ccctgattga ccagcaaaaa
     5941 atccgggaac agttgcgatc ccgggaagag accctgaaga atgataatgt ggctgcgcgt
     6001 gcatcaaatg aagccgaact gctgggatac gggcagggag aacgagccag ggaacgcatg
     6061 cgggagttgc agcagatccg tgacagcttc cgccagaagg atgcggacct tcagtctcag
     6121 tatcagaccg gggatatcag tgaggatttt tacagacagg cacgggcaca gaacgcgcag
     6181 tatctgagcg aacgccttaa ggaccaggca gccttttatg ccgaatcgga tgcgcagcgt
     6241 gcggactggc agaaaggctt gcaggagggg ctcagtaact gggtggacaa tgcatccgat
     6301 tacgcctcac aggcagcaca gcttgcgacg gagggtatct cagggatggt gaataacatc
     6361 acggagatgc tgaacggaaa caaagtggaa tggcgcaact gggcctcatc cgtactgcag
     6421 gaaatctcaa aagttcttat gaatgctgcg attgtcaacg gaattaagac ggcggcaaac
     6481 ggtatgtccg gtgcgggagg atttattggc agcattggtg actggctggg cggtgcggtg
     6541 gccaatgcaa aaggcggcgt gtatacctcg gcaaacctga gtgcgtacag caacagcatt
     6601 gtggacacgc ccacgtactt tgcgtttgca aaaggggccg ggctgatggg ggaagccgga
     6661 cctgaagcca ttatgcccct gacccgggcg gcggatggct cgctgggcgt acgcgcggtg
     6721 ggcagtatga acggcagtgc cggtctggtg tattccccgg tctaccacat cgccattcag
     6781 aatgacgggg ctaacggaca gataggggcg gaggcggcag gcagtcttgt gcagctgatt
     6841 gaccagcggg tgcaggcggt gatgctgtcc atgcgacgtg acggaggaat gctgagtggc
     6901 tgagataaaa acgctgcatc tggtcccgcg tgaagggatg caggtgagtg agaaaccgtc
     6961 ggtggtgagg gttcggtttg gtgacggtta tgaacagcgc cgaccgacgg gacttaatgc
     7021 cagactgaag acgtttcagg cggtgttccg ggtgacggat gaaccaaccc ggcgctggct
     7081 ggatgaattt ttatcgtggc atggtggtta ccgtgccttt ttgtggcgac cgccgaaaca
     7141 taacctgacg gtgagggtgg tgtgccggga gtggagcgtc acagataacg ccaggcacag
     7201 tgatttcagt tgtacgattg agcaggtggt gaactgatgc aggatattcg cgaagaaagt
     7261 ctgaacgagt cggttaagtc agagcagtca ccgcgggtgg tactctggga aatcgacctg
     7321 acggtacagg gcggtgagcg gtattttttc tgtaatgagc tgaatgaaaa aggggagccg
     7381 gtcacctggc aggggcggca atatcaggca tacccgattg acggcagcgg ttttgagatg
     7441 agcgggaagg gcagcagtgc cagaccgtcg ctgacggtgt ccaatctgtt cggtctggtc
     7501 accgggatgg cggaagacct gcagagtctg gtgggggcca cggtggtccg ccgccgggtg
     7561 tatgcccgtt ttctggatgc ggtgaatttc gttgcgggca atccggaggc ggacccggag
     7621 caggagctga gtgaccgctg ggtggtggag cagatgtcgc agctgacagc catgacggcc
     7681 tcgtttgtgc tggctacacc gaccgagacg gatggggcgc tgtttcccgg tcgcatcatg
     7741 ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat gcgggtataa cggtcctgcg
     7801 gtggcggatg agttcgacaa ccccaccacg gatatccgta aggacagatg cagcaagtgc
     7861 atgcgcgggt gtgaactgcg caggaatgtc ggcaattttg gcggtttcct ttccattaat
     7921 aaactttcgc agtaaatccc ggtttatgac acagactgaa tcagcgattc tggcgcatgc
     7981 ccggcggtgt gcgcctgcgg agtcgtgcgg cttcgtgata agcacgtcgg agggggagtg
     8041 gtatatccct tgtgtgaata tttctgcaga gccggaggcg tattttcgta tcgcaccgga
     8101 agactggctg cgggcagaga tgcaggggga gattgtggca ctggtccaca gtcatcccgg
     8161 tgggctgccc tggctgagcg aggctgaccg gcggctgcag ataaaaagcg cactgccctg
     8221 gtggctggtc tgccggggtg acattcacaa attccgctgt gtgccacatc tgacaggacg
     8281 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     8341 ggggactgaa atgccggatt ttcatcgcga ggatgactgg tggcgcaacg gtcagaacct
     8401 ttacctggac aatatggcgg tcaccggctt ttaccgggtg cccctgtcct ctgcacaggc
     8461 gggcgatatt ctgctgtgct gctttggtgc ttcggtaccg aaccatgccg ccatttactg
     8521 cggcaacggt gagctgcttc accatctgcc tgaacaactg agtaaacggg agaggtattc
     8581 cgaaaaatgg caacgacgaa cgcattctgt ctggcgtcac cgccactggc acgcatctgc
     8641 cttcacgggg atttacaacg atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg
     8701 gaagccatcc gggcgctgtc gttacaggtg ccgggctttc gccgtcagat gaacgaaggc
     8761 tggtaccaga tacgtattgc cggttatgac acggcaccgg aggcggtgta cgcccgtctt
     8821 cacgaacagc tgggtgaggg aacggtcatc catattgtgc cgcgactggc cggggccgga
     8881 aagggtggac tgcagattgt gctgggggcg gcagccatcg tgggctcttt cttcactgcc
     8941 ggggcatcaa tggcgttatg gggttcagcc ctggcagccg gtggtttttc tgccaccacg
     9001 atgctgtttt cacttggagc cagcatgatt ctgggcggtg tggcccagat gctggccccg
     9061 aaggcaaaaa caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc
     9121 tcgctggata acatgattgc ccaggggaac ccgatgccgg tgccttacgg ggaaatgctg
     9181 gttggctccc gccgtatatc ccaggacatc agcacccgtg atgaaggcgg gggcggaacg
     9241 gtcgtggtta tcgggcgaca gggataaaac ataaaaaaat cccgcagtga tcgcggagct
     9301 gcggggacag acaaatgaag atcaatgtta aggagttgtt tttgttactc gggcaaaaaa
     9361 acactaacgc agcgaaatta taagcgccac agtcagtgtg tgaaaatgtg aagatattca
     9421 gaatttttat gccattaccg gttttaacca acaggattat cggtgggcat gaaagaaaac
     9481 cccggtatct gctgataccg gggtttctct ttagcatggc agaaatgtgt ttcatgcttt
     9541 tcgggcgaag gatatccgac ttctgtacgg aatggcaagt ggcggttaat ttattcaggg
     9601 gaaggctgta tgggaaaagg tggcggtaag gcacacacgc ctcgtgaggc gaaggataat
     9661 ctcaaatcca cgcagatgat gagcgtgatt gatgcgattg gtgagggacc gatagaaggt
     9721 ccggtgaagg gactgcagag tattctggtg aacaaaaccc cactgacgga cacggacggc
     9781 aatcccgtga tacacggtgt gacggcggtc tggcgcgccg gggagcagga gcagacacca
     9841 ccggaaggct ttgagtcctc cggagctgaa accggactgg gcgtggaagt gacgaaggca
     9901 aaaccggtga cgcgcaccat tacgtccgcg aacattgacc gcctgcgggt taccttcggg
     9961 gtgcagtcac tggtggagac cacctcaaag ggtgaccgta acccggcatc cgtccgcctg
    10021 ctgattcagt tacagcgtaa cggtaactgg gtgacagaaa aggacgtcac cattaacggc
    10081 aagaccacct cacagttcct ggcctcggtg attctggata atctgcctcc ccggcccttt
    10141 aacatccgga tggtcaggga gacggcggac agcaccacgg accagctgca gaataagacg
    10201 ctgtggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggccatt
    10261 gtggggctgc aggtggatgc ggagcagttc ggcggccagc agatgacggt gaactaccat
    10321 atccgcggtc gcatcatcca ggtgccgtca aactatgacc cggaaaaacg cacgtatagt
    10381 ggtatctggg acggcagtct gaaaccggca tacagcaaca acccggcctg gtgtctgtgg
    10441 gacatgctga ctcacccgcg ctacggcatg ggaaaacgtc tgggggcggc ggatgtggac
    10501 aagtgggcgc tgtatgccat cgggcagtac tgcgaccaga cggtcccgga tggtttcggg
    10561 gggaccgagc cgcggatgac ctttaatgcg tacctggcac aacagcgtaa ggcgtgggac
    10621 gttctcagtg atttctgctc tgcgatgcgc tgtatgccgg tatggaacgg tcagacgctg
    10681 acgttcgttc aggaccgccc gtcggatgtg gtgtggccgt acaccaacag cgatgtggtg
    10741 gtggatgata acggcgtggg attccgctac agcttcagtg ccctgaagga ccggcacacg
    10801 gcggtggagg tgaattacac cgacccgcag aacggctggc agacctccac ggaactggtg
    10861 gaagacccgg aagccatact gcgctacgga cgcaacctgc tgaagatgga cgcgttcggc
    10921 tgtaccagcc gcggtcaggc ccaccgtgcc ggactgtggg tgataaagac cgaactgctg
    10981 gaaacgcaga cggtggattt cacgctcggg tctcaggggc tgcggcacac acccggtgac
    11041 attattgaaa tctgtgataa tgactatgcc gggaccctga ccggcggacg tgtcctgtcc
    11101 attgatgctg ccacccgcac cctgacgctg gaccgtgagg ttaccctgcc ggagacaggt
    11161 acatcggcgg tgaacctgat taacggcagc ggtaagccgg tgagtgtgga catcaccgca
    11221 caccccgcgc cggaccggat acaggtcagt accctgcctg atggtgtgga gacatacggg
    11281 gtgtggggac tctccctgcc gtcactgcgc cgtcgcctgt tccgctgtgt ctccatccgg
    11341 gaaaacacgg acggcacctt tgccatcacg gcggtgcagc acgtaccgga aaaagaagcc
    11401 atcgtggata acggtgcccg ctttgagccg cagtcaggtt ccctgaacag cgtcatccca
    11461 ccggcagtgc agcacctgac ggtggaggtg agcgcagctg acggccagta tctggcgcag
    11521 gcgaaatggg acacgccgcg ggtggtgaag ggtgtgcgct tcagtctgcg cctgaccagt
    11581 ggtaagggaa cggatgccag actggtgacc accgccatca ccgcagacac ggagcaccgt
    11641 ttcagcggcc tgccgctcgg ggaatacacc ctgacggtgc gggcgataaa cagctatggc
    11701 cagcagggtg aacctgccac caccaccttc cggattgccg caccggcagc accgtcgcgg
    11761 attgagctga cgccgggcta ttttcagata accgcaacgc cacatcttgc cgtttatgac
    11821 ccgacggtac agtttgagtt ctggttctcg gaaaagcgga ttgcggatat caggcaggtt
    11881 gaaaccgcag cccgctatct tggctcggcg ctgtactgga tagctgccag tatcaatatc
    11941 aaaccgggcc atgattatta tttttatatc cgcagtgtga atactgttgg caaatcggca
    12001 ttcgtggagg ctgtcggtcg ggcgagcgat gatgcggaag gttacctgga ttttttcaaa
    12061 ggagaaatcg ggaaaacaca tctggcccag gagctgtgga cgcagattga taacggtcag
    12121 cttgcgccgg acctggctga aatcaggacg tccattacga atgtcagcaa tgaaatcacg
    12181 cagaccgtca ataaaaaact ggaaaatcag agtgcggcaa tccagcagat acagaaagtt
    12241 caggttgata caaataataa cctgaacagc atgtgggccg tgaaactgca gcagatgcag
    12301 gacggacgcc tttatattgc gggtatcggt gccggtattg agaatacgcc agcaggaatg
    12361 cagagtcagg tgctgctggc ggcagacagg attgcgatga ttaatcctgc gaatggcaac
    12421 acaaagccga tgtttgttgg tcagggcgat cagatattta tgaatgaagt gttcctgaaa
    12481 tatctgacgg ctcccaccat taccagcggc ggtaatcctc cggcattttc cctgacaccg
    12541 gacgggcggc tgacggcgaa aaatgccgat atcagcggta acgtgaatgc gaactccggg
    12601 acgctcaaca acgtcacgat taacgagaac tgtcgggttc tgggaaaatt gtccgcgaac
    12661 cagattgaag gcgatctcgt taaaacagtg ggcaaagctt tcccccggga ctcccgtgca
    12721 ccggagcggt ggccatcagg aaccattacc gtcagggttt atgacgatca gccgtttgac
    12781 cggcagattg ttattccggc ggtggcattc agcggcgcta aacatgagaa agagcatact
    12841 gatatttact cctcatgccg tctgatagtg cggaaaaacg gtgctgaaat ttataaccgt
    12901 accgcgctgg ataatacgct gatttacagt ggtgttattg atatgcctgc cggtcacggt
    12961 cacatgacac tggagttttc ggtgtcagca tggctggtaa ataactggta tcccacagca
    13021 agtatcagcg atttgctggt tgtggtgatg aagaaagcca ctgcaggcat cacgattagc
    13081 tgaattttat aacccagata cgggcgccag aaatggcgcc ttttttattg cagaaaagcg
    13141 agaggtaatt atgcgtaaat tatgtgctgt tattttgtcc gcagtagtct ggcaggtcgc
    13201 cgctgctacg ccagcgagtg cagcagaaca tcagtccacg ctgagcgcgg ggtatctcca
    13261 tgcctcgacg aacgttcccg gtagtgatga tctgaacggg attaacgtga aataccgtta
    13321 tgagtttacg gacgcgctgg ggctgattac gtccttcagt tatgccaatg ctgaggatga
    13381 gcaaaaaacg cgctacagcg atacccgctg gcatgaagat tccgtgcgta accgctggtt
    13441 cagcgtgatg gcggggccgt ctgtacgcgt gaatgaatgg ttcagcgcgt atgcgatggc
    13501 gggtgtggct tacagccgtg tgtcgactt
//
LOCUS       DACADA010000122        13526 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.124, whole genome shotgun sequence.
ACCESSION   DACADA010000122 DACADA010000000
VERSION     DACADA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.124"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(300..725)
                     /gene="arsC"
                     /locus_tag="ISB17_003503"
     CDS             complement(300..725)
                     /gene="arsC"
                     /locus_tag="ISB17_003503"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAP1981394.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1060..1143
                     /locus_tag="ISB17_003504"
     CDS             1060..1143
                     /locus_tag="ISB17_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAP1981395.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(1197..2549)
                     /gene="gorA"
                     /locus_tag="ISB17_003505"
     CDS             complement(1197..2549)
                     /gene="gorA"
                     /locus_tag="ISB17_003505"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP1981396.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2621..3463)
                     /locus_tag="ISB17_003506"
     CDS             complement(2621..3463)
                     /locus_tag="ISB17_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAP1981397.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3666..5708
                     /gene="prlC"
                     /locus_tag="ISB17_003507"
     CDS             3666..5708
                     /gene="prlC"
                     /locus_tag="ISB17_003507"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAP1981398.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5716..6468
                     /gene="rsmJ"
                     /locus_tag="ISB17_003508"
     CDS             5716..6468
                     /gene="rsmJ"
                     /locus_tag="ISB17_003508"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAP1981399.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPAQLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6517..7986)
                     /gene="dtpB"
                     /locus_tag="ISB17_003509"
     CDS             complement(6517..7986)
                     /gene="dtpB"
                     /locus_tag="ISB17_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAP1981400.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     QLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8303..8737)
                     /gene="uspA"
                     /locus_tag="ISB17_003510"
     CDS             complement(8303..8737)
                     /gene="uspA"
                     /locus_tag="ISB17_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAP1981401.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9128..9463
                     /gene="uspB"
                     /locus_tag="ISB17_003511"
     CDS             9128..9463
                     /gene="uspB"
                     /locus_tag="ISB17_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAP1981402.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9534..11033)
                     /gene="pitA"
                     /locus_tag="ISB17_003512"
     CDS             complement(9534..11033)
                     /gene="pitA"
                     /locus_tag="ISB17_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAP1981403.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11265..12458
                     /locus_tag="ISB17_003513"
     CDS             11265..12458
                     /locus_tag="ISB17_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP1981404.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12545..>13526)
                     /locus_tag="ISB17_003514"
     CDS             complement(12545..>13526)
                     /locus_tag="ISB17_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HAP1981405.1"
                     /translation="EFVLTGSGQSEYFVAGHVLISLSAICLALFTTAFIIISQLTHGM
                     NKFYNKLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMIAACVSTVAA
                     SSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSVILLRSADIT
                     PHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILLGSLTIIFGI
                     YVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCL
                     FCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         3447 a         3429 c         3560 g         3090 t
ORIGIN      
        1 tatttattgt gcaaaccact gatagatcat aatccagtac atctttacag tgctgcactg
       61 tatcgctgat aactaagcag ctcactgata caccatgctg gacgcgataa ttggaatctc
      121 acgcagggag tactgtgtaa atagcaaaat cgcctgatgc gctacgactg tcaggcctac
      181 aaataacata caattcatcg aattatcata acgttgtagg tcggataaga cgcgtcagcg
      241 tcgcatccga cataaataaa gcgcattttt ctaacaacct gtgggggaat atagccgctt
      301 tatttcaggc gattacccgc ctcatcaacc actttctcgc catcttcctt ggtgaacgcg
      361 cctttttgcg catctggcag aatttccagc acgacttctg aagggcggca caggcgagtt
      421 ccctgtggcg tcaccacaat cgggcgatta atcagaatcg ggtgctgaag cataaagtcg
      481 attaactgat cgtgagtaaa tttatcttct gcaaggccca gttcctcata cggctcgacg
      541 tttttacgca gcaacgcgcg tacggtaatc cccatatcgg caatgagttt taccagctca
      601 tcgcgcgtcg gtggtgtttc cagataatag ataatggtcg gttctgtacc gctgttgcgg
      661 atcatctcca gcgcattacg cgacgtaccg caggccgggt tgtgataaat ggtaatgttg
      721 ctcatatcag tatctcatta caatgtgaaa gagaggtgta gcgccagcgc caccagcgtt
      781 acaaaccaaa gcggacaagt caaaaacaca tatggtttaa cgaatatgta agtgtagaga
      841 aaggtgtagg tgtgaattaa gcgttgggca ttcatttgaa aggaggtctg aatcaggacg
      901 taaaaaaacc gacttcgcgt cggttttttt actgttcagc cctgagttgg tggctctggc
      961 tggagtgaga tagcccacat ctaacaatga gacatgctcc gtgacaggca atggatgagt
     1021 aagcggatgc attctcactc catcgcatgg agaaaacggg tgattgataa agcaatcatc
     1081 gttcttgggg cgttaattgc gcagctggaa ctgatccgct ttctgcttca gcttctgaac
     1141 tgatagcgga aacgtagcta agggctaaga gcacactact cttagccctt taacatttaa
     1201 cgcattgtca cgaactcttc tgctgctgtc gggtgaatgg caaccgtgtt gtcgaagtct
     1261 tttttggttg cacccatctt cagcgccacc gcgaagccct gcaacatttc gtccatacca
     1321 aaaccaatgc cgtgaatacc gacaatcttc tcttccggtc caatgcatac cagtttcatg
     1381 cgacacggct ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac
     1441 actttcacct gatcgtcgcc gtactgctcg cgcgcctgcg gctccgttaa accaacagta
     1501 ccaatcggcg gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc
     1561 ggcttgttat taaacaggcg ttcagagaga cggcgacctg ctgcaactgc caccggagtc
     1621 agttccactg cacccgtgtt atcgcccacc gcgtaaatac cttcaacgtt ggtgttttga
     1681 tatttatcga cgacgatata gcctttttcg ttggttttaa cgccagcggc ttccaggttg
     1741 atgttgtcat tggcaggctc gcgaccaatc gcccaaatca ggcaatccac cgtttcactg
     1801 cgaccatctt ccagctccag cgtcaggctg ccgtcggcat ttttcaccac cgctttcggg
     1861 atggcgttgg tatgcaactg tgggccttcg gcgttcatca cttcgaccag cgtttcagag
     1921 atcatcgggt cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgtcttcgca
     1981 ccgaggccgt taatcacgcc cgccagttca acggcgatgt aacccgcgcc aacaaccgct
     2041 acgcgctctg gcaaagccgg aagggcgaag aagccatcag aatcaatacc gtattccacg
     2101 cccggaatat ccgggtggct cggacgacca ccggtggcga tcagaatatg atcggccgtg
     2161 atggtttcgc cgttgacttc aatggttttg gcatcaacga agcgggcaaa gcctttgatt
     2221 acatcaacgt tatttttacc gagcacgttc tcataggaag tatgaatacg gtcgatatag
     2281 gcggtacggc tggcgatcaa tgtttcccag ttgaatttat tgatagtggt atcaaagccg
     2341 taatccgggc cgtacatatg gatcgcttca cggatttgtg ccgcgtgcca catcactttt
     2401 ttcggcacac agccaacatt tacgcaggtg ccgcccagct ctttggcttc aatcagtgcg
     2461 catttctggc cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg
     2521 atggcgatgt aatcatagtg tttagtcatg acatattgtc cttatcgttg attaccgcga
     2581 ttgtagcgcg agacgtaata ggtgccagca atggctgcaa ttactctggc acgatccagc
     2641 ttacggtggc gtgcccggtg cctgctggaa ccagtttgct gtgcagccac ggcagcacgt
     2701 tattcatctg ctgttccagc ttccacggcg ggttaatcac aatcatgccg gaagcggtca
     2761 tgccacggcg atcgctgtct ggcagcaccg ccagttcaat ttgcagaatt ttgcgaatgc
     2821 cggtcgcttc caggtcgtgg atcatgcgct taatctgctg gcgcagcacc actggatacc
     2881 acaatgcgta agtgccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgaccaccg
     2941 cctgatagtc ggttttcatt tcatacggcg gatcgataag gattaaacca cggcgggaaa
     3001 ccggcggtag tttggcctta agctgctgga aaccgtcggc tttttcaacg cgcgcacgat
     3061 tgtctttctg gaattccgaa cgtaacaacg gataatcgct cgggtgcagt tcagtcagtt
     3121 gcaggctgtc ctgttcacgc agtagctggc gcgcaatcaa cggcgaaccg gggtaataac
     3181 gcaactgccc gctacggttg aagtgtttta ccacattgat gtacgcctcc agctccgcag
     3241 gcaaatcgtc ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg
     3301 catgttcgct gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg
     3361 gtttatcttt ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt
     3421 cggcgtggtt gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg
     3481 taagtaaaaa tcggcccgta ttcggggcgc acaaaagtct atcaggacag tataccgaaa
     3541 agaggccgcc gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat
     3601 gctacaaaca ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg
     3661 cgctaatgac gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc
     3721 cggaacatgt cgttccagcc gtgactaagg cgctgaacga ctgccgcgaa aatgtggagc
     3781 gcgtagtagc gcaaggggca ccgtacacct gggaaaatct ctgccagccg ttggcggaag
     3841 tggacgatgt gctggggcgt atcttctccc cggtcagcca tctgaactcg gtgaaaaata
     3901 gcccggaact gcgtgaagcg tacgaacaaa ccctgccgct gctgtcagaa tacagcacct
     3961 gggtaggaca acatgaaggg ctgtataaag catatcgcga cctgcgcgat ggcgatcatt
     4021 acgccacgct gaacacggcg cagaaaaaag cggttgataa cgcactgcgc gacttcgaac
     4081 tctctggcat cggtctgccg aaagagaaac agcagcgtta cggcgaaatt gccactcgtc
     4141 tttctgaact gggcaatcag tacagcaaca acgtcctcga tgcgacgatg ggctggacca
     4201 aactcgttac cgacgaagcg gagctggcgg ggatgccaga aagcgcgctg gctgcggcaa
     4261 aagcccaggc cgaagcgaaa gagctggaag gttatttgct gacgctggat atcccaagct
     4321 acctgccggt aatgacctac tgcgacaacc aggccctgcg tgaagagatg tatcgtgctt
     4381 atagcactcg tgcttccgat caaggtccga acgccggtaa gtgggataac agcaaggtga
     4441 tggaagagat cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg
     4501 ccttcaaatc acttgccact aaaatggcag aaaacccgca gcaggtgctg gatttcttaa
     4561 ccgatctggc aaaacgcgcg cgcccgcaag gcgaaaaaga gctggcacaa ctgcgcgcct
     4621 tcgccaaagc cgaatttggc gtcgatgagt tgcaaccgtg ggatatcgct tactacagcg
     4681 aaaaacagaa acagcacctc tacagcatca gcgatgaaca actgcgtccg tacttcccgg
     4741 aaaacaaagc ggttaacggc ttgtttgaag tggtgaaacg tatttacggc atcaccgcta
     4801 aagagcgtaa agatgttgat gtctggcatc cggatgtacg tttcttcgaa ctatatgacg
     4861 agaacaacga actgcgcggt agcttctacc tcgacctgta tgcccgtgaa aacaagcgcg
     4921 gcggggcgtg gatggatgac tgcgtaggcc agatgcgtaa agccgacggt tcgctgcaaa
     4981 aaccggtcgc gtatctgacc tgcaacttca accgcccggt aaatggtaaa ccggcgctgt
     5041 ttacccatga cgaagtgatc accctgttcc acgagttcgg tcacggcctg caccatatgc
     5101 tgacccgcat tgaaaccgct ggtgtttccg ggatcagtgg ggtgccgtgg gatgcggtcg
     5161 aactgccgag ccagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct
     5221 ccggtcacta tgaaaccggc gagccgctgc cgaaagagtt gctggataaa atgttggcag
     5281 cgaaaaatta ccaggcggcg ctgtttattc tgcgccagct ggagttcggc ctgttcgatt
     5341 tccgcctcca tgccgagttc cgtccggatc agggggcgaa aatcctcgaa actctggcag
     5401 aaatcaagaa actggttgcc gtggtaccgt ctccgtcatg gggccgtttc ccgcatgctt
     5461 tcagccacat ttttgccggt ggttatgcgg caggttacta cagctacctg tgggccgatg
     5521 tactggcggc agatgccttc tcgcgttttg aggaagaggg cattttcaac cgtgaaactg
     5581 ggcagtcgtt cctcgacaac attctgagcc gtggcggttc agaagagccg atggagctgt
     5641 tcaaacgctt ccgtggtcgt gaaccacagc tggatgcgat gctggagcat tacggcatta
     5701 agggctgatc attcagtgaa aatctgctta attgatgaaa caggcgccgg agacggcgcc
     5761 ttatctgttc tggcggcccg ctgggggctg gaacacgatg aagacaacct gatggcgctg
     5821 gtgttaacgc cggagcatct ggaactgcgc aagcgtgatg agccgaaact tggcggcatc
     5881 tttgttgatt ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcgga
     5941 gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgcc
     6001 accgcagggc tggggcgcga tgcctttgtg ctggcttcgg tcggctgccg cgtgcggatg
     6061 ctggagcgta atccagtggt tgccgcgttg ctcgacgatg gtctggcgcg tggttatgcg
     6121 gatgcggaaa tcggcggctg gttacaggaa cgtttgcagt tgattcacgc ctccagtctg
     6181 acggcgctga gcgatatcac cccgcgtccg caggtagttt atcttgaccc aatgttcccg
     6241 cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgcg tgtttcagtc gctggtgggg
     6301 ccggatcttg atgccgatgg attgctggag cctgcgcagt tgctggcaac caaacgcgtg
     6361 gtggtaaagc gtccggacta tgcgccgccg ctggcgaatg tcgccacgcc aaacgcggta
     6421 gtcactaaag ggcatcgctt tgatatttat gccggaacgc cggtgtaaaa gtaaagcggg
     6481 gtagcgataa cgctaccccg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat
     6541 catgcgtttc agccacggca ccatcaatag catcactact gcaacgccca gcgtaaccag
     6601 gccaatctta ccaaacacgt tggtatagac gggcaacgtc tcaagtggat cggtaatgtt
     6661 gtctggtact gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgcgt
     6721 caggaaccac atcccgagaa taaagcccat caaatgctgc ggcaccaggg cagcaatcat
     6781 cgccaggcca agggcgctaa taaacagttc acctaagctc tggaataagt acaccagcac
     6841 gataaaccat ggcgatgtca gcccttgggc atccgcaaac cacattccag ccgctgcagc
     6901 cgtcaaaaag cccagtgagc acataaacat gccgagagta aacttcatcg gcatcgagag
     6961 atctttgcct ttgttaccca gttgcgtgta aatgcctgcc agtattgggc tggcgagtac
     7021 cacccagaac gggttaagcg cctggaagct gaccgggttg atggaaaagc cgagaatttc
     7081 atgatgcacg ttgttgatgg caaagaagtt cagcgacgtt ggcatctggg cgtagagaat
     7141 gtaaaacacc accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt
     7201 tttatccagc ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat
     7261 cagcaccaga ttggcgactt ctacgttgtg catcagccag gcgcagacga agatcatcac
     7321 cacgctgcca agtaatacgt acaacagttt actgaagctc atcggccgga agtcgggttc
     7381 agaaccaata tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa
     7441 ccctgcaccg cacaggttgt aggtgactga atagccgaat ttatcagcga tcacaggggc
     7501 cagcgataac gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgcgcc
     7561 atcaagccgt ggatctttcg gcggatagca tttcgaaagc aagctggctg ggttagcttt
     7621 aaacaggccg ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag
     7681 tagcgacatg ccggtcatga agtagccaat cgccagcaca agtgctccga gaacaatggt
     7741 gcgtttggtt cccagcaggt ggtcgccaac atagccacca atggaaatga ggccatagac
     7801 cagcgcagca aaagcaccaa aagtgacaaa agcctgctct tgcgagaacc caagctgttt
     7861 aacgaagaaa accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc
     7921 gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt
     7981 attcatattt attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg
     8041 cgcgaaaaag tttacatctg tcagagattc ttaatattga tgcggcgaat agattaatac
     8101 actattacaa cagaaaataa ccagctgatc acactaatga tagaaaatat gatggtgtta
     8161 aatgttgtat tggcaggggt tttaattgcg tacgcagacc gcaggccaga taaggcgttt
     8221 acgctgcatc aggtaacaag gcgcgccaaa aagcaaaacg cccgcatatg cgggcgttca
     8281 ggaacacgtc gtggagggaa gattattctt cttcgtcgcg cagcggaaca atcagcatat
     8341 caacgtgaac ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct
     8401 ggtggtgacc acaaaccacc agatccatat cgtatttctt gattgcatcg accagtacct
     8461 ggcccagatc gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttggttgaaa
     8521 gctcggtcag cgcatgatgt gtctcttcag agatgcgttt ctgcatatca cccagattca
     8581 catcgataag cccggtgtat aggtcagagt agtttacatc tacgtggatc agagaaactt
     8641 tcgcattgta ggggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg
     8701 agaggtcgac cgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt
     8761 gtcgatgact ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta
     8821 cgtgcgcggg gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc
     8881 gacccggatg atccgtcaat ccgccttgct tactcgttag ataaacaaat tttcgacatg
     8941 aatatattga tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta
     9001 tagagccgct cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg
     9061 attggcagcg tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg
     9121 aggaggtatg ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa
     9181 catggcgcgc tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc
     9241 attgctctat caatatgttg atggaggggg cttttttacc tcacatggcc aaccaaacaa
     9301 acaggtgcgt ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga
     9361 gtttattcgc cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg
     9421 tctggtggtg gtcagcctga ttgcattgat gatttggcat taagcacttc agcaataatg
     9481 acgcataaaa aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta caattacagg
     9541 aactgcaagg agagccagta cagcccgccg gaaagcagta ccgcagccgg aagggtaaac
     9601 acccaggcca tcagaatact ggttacggtt ttacgctgta agccgccgcc atctaccacc
     9661 atcgtccccg cgacagaaga ggagagtacg tgagtagtgg aaaccggcat cccggtataa
     9721 cttgccaggc cgatagacac agccgccgtc atctgggcag acatcccctg agcgtaggtc
     9781 atgcctttct taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc
     9841 gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggcgc atactcgatg
     9901 gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacgcca
     9961 ggcattttca ccactttatc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg
    10021 ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtagtcaa catgcctttc
    10081 aggcggttga gcgcgttaat ggtattcgac ggatggcaat ggaactccgc gggttgcgtt
    10141 gcgccagctt ccggagccgg tactaactga tcagcaccgg tcgcttgttt cagcagtgca
    10201 ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg
    10261 tagccagtgg cattcatgtt caccacgaag cctgctggtg cgacgccaat caataccaac
    10321 ataaccagac caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata
    10381 gcggaaagga tcagcgcaat acgcgtccag aacggcggct ttttcttgcc gtctttcttt
    10441 tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga
    10501 cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga
    10561 gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc
    10621 atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat
    10681 gcaggtaaac caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag
    10741 aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata
    10801 tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaatacc
    10861 gccgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg
    10921 gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca
    10981 agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt
    11041 tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa
    11101 gtgaaatata gacttttttt gatttgcctc ctgtatggat tccactcaaa aaataattat
    11161 cttatataat tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag
    11221 gaatctttac aattcacgcc cgttttttct tagaggagcg caacgtggaa aggtttgatg
    11281 ccattattat aggcgctggt gcggcaggta tgttctgttc tgcgctggca ggtcaggcag
    11341 gacgccgggt tctgctgatc gataatggta aaaaaccagg gcgcaaaatc ctcatgtctg
    11401 gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc aggcgcttat ctgagccaga
    11461 atccgcattt ttgtaagtct gcactcgcgc gttttaccca gtgggatttc attgatctgg
    11521 tcaataaaca cggcatcgcc tggcacgaga aaacgttagg gcagctcttc tgcgatgact
    11581 ccgcgcagca gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtggccttca
    11641 gattgcgtag cgaagtgctg agcgtggcga aggatgatac aggcttcacg cttgaactga
    11701 acggcatgac tgtcggttgc gaaaagctgg tcattgcgac cggtggactg tcaatgccgg
    11761 ggctgggcgc atcgccgttt ggttataaga ttgccgaaca attcggcctc aacgtgctgc
    11821 cgacccgtgc gggtctggtg ccattcactc tgcataaacc gttgcttgaa gagttacagg
    11881 tgctggcggg cgtggctgtg ccttccgtga ttaccgctga aaacggtacc gttttccgtg
    11941 agaacttact cttcacccac cgcggcttat ctggaccggc ggtgttgcag atttccagct
    12001 actggcaacc gggtgaattt gtcagcatca atctgctacc ggatgtggac ctcgaaacct
    12061 tcctgaatga gcagcgtaat gcacatccga atcaaagcct gaaaaacacg ctggcggttc
    12121 atctgccgaa gcggttggtt gaacggttac aacaactcgg gcaaatcccg gatgtctcgc
    12181 taaaacagct caacgtgcgt gaccaacaga cactgattag cacattgact gactggcgcg
    12241 tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca
    12301 ccaacgaact ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg tacttcatcg
    12361 gcgaagtgat ggacgtcacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga
    12421 gtgcatgggc ttgtgcgcag gatttggtgg cagaataaga agcaaaatgc gctctcgata
    12481 ccagagggat taacctgaat aaaaccagat aaggcgctca acgccttatc tgaagccttt
    12541 tttattattt tttcgctgaa cccgcttcta atatagaaac gatggaaaac agtgtaaaac
    12601 aaactgcacc taagccaacc agaactcgcg aaggaataaa gtaagccata tcggtttgcg
    12661 caatttcagc caggaacgca gcaaggaaca gacaaaacag acaggtaaat accggaatca
    12721 tgggtattct gttggctaac gaacaggtgc gtcgccatac cagcgctaac aaccacactt
    12781 tggagaaaat actgtagcag atcatcccca ggcaaatgag aataatgccg ggggccaggc
    12841 gggcgcttgc atctgaactg attaatacgt agataccaaa aataatggta agtgagccaa
    12901 gcaaaataac ccaatagcac cacagccaat gttctttcac tgagaacgta ttccgcgttt
    12961 gatggacaat agtggcgaca aggccaataa gacaagcgca aattgccgtt aaccccatta
    13021 gtacgtgacc tgcaacataa tgtggtgtga tatctgcact acgtaacagt atgactgacc
    13081 aaatgaaacc aaagagagta agcagaacgg gaacggcaat taaacaatta ccgattgttg
    13141 aagaataggc ctgcaatggc gtaccgtcac tcttactgcc cgaggcgttt ttggggatca
    13201 acagaaaatg tccggaagat gccgctacgg ttgatacaca agcggcaatc atgccaacgc
    13261 cgaaaataac atgaccggcg acaaattcat ctgccatcac attattgctt gccaacagtg
    13321 accatcccca aatcatagtt gtagcagaac cggcataacc aataacagga aataacttgt
    13381 tgtagaattt attcatgccg tgagttaact gagatataat aataaatgcg gtagtgaaca
    13441 atgccagaca tatcgcagaa agagaaatca gaacatgccc tgcaacgaaa tattcacttt
    13501 gcccagaacc ggttaataca aactca
//
LOCUS       DACADA010000123        13293 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.125, whole genome shotgun sequence.
ACCESSION   DACADA010000123 DACADA010000000
VERSION     DACADA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.125"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="ISB17_003515"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="ISB17_003515"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAP1981406.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1380..2186
                     /gene="tcdA"
                     /locus_tag="ISB17_003516"
     CDS             1380..2186
                     /gene="tcdA"
                     /locus_tag="ISB17_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAP1981407.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="ISB17_003517"
     CDS             complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="ISB17_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAP1981408.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="ISB17_003518"
     CDS             complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="ISB17_003518"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAP1981409.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4077..4304
                     /locus_tag="ISB17_003519"
     CDS             4077..4304
                     /locus_tag="ISB17_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAP1981410.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4655..5572
                     /locus_tag="ISB17_003520"
     CDS             4655..5572
                     /locus_tag="ISB17_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAP1981411.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGENPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5591..5986
                     /locus_tag="ISB17_003521"
     CDS             5591..5986
                     /locus_tag="ISB17_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAP1981412.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5979..7079
                     /gene="rlmM"
                     /locus_tag="ISB17_003522"
     CDS             5979..7079
                     /gene="rlmM"
                     /locus_tag="ISB17_003522"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAP1981413.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="ISB17_003523"
     CDS             complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="ISB17_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAP1981414.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="ISB17_003524"
     CDS             complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="ISB17_003524"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAP1981415.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="ISB17_003525"
     CDS             complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="ISB17_003525"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAP1981416.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATSIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9863..11638)
                     /gene="fucI"
                     /locus_tag="ISB17_003526"
     CDS             complement(9863..11638)
                     /gene="fucI"
                     /locus_tag="ISB17_003526"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAP1981417.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11671..12987)
                     /gene="fucP"
                     /locus_tag="ISB17_003527"
     CDS             complement(11671..12987)
                     /gene="fucP"
                     /locus_tag="ISB17_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAP1981418.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3213 a         3294 c         3493 g         3293 t
ORIGIN      
        1 gtggatgcgc actatactct gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttggcat gattttgaac aaaaacaaat cactagagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggtggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaat gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgtcgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 tcagggcgag ttccagtatc cgatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcag gagcgctgag tgataaatat attctcgctt acagtaactc
      721 cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgttg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcactggggc gaaacacaca gtgaagctga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gtgtctgggt
     1261 gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcaccc tttttaattc tgaggtgcta
     1381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
     1441 gtgaaaaagc attgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1501 tcggttcctg ggcggcggaa gcgctagcgc gcacggggat tggtgcaatc acgcttatcg
     1561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     1621 tcgggctggc aaaagcggaa gtcatggcgg agcgtattcg ccagattaac ccggagtgcc
     1681 gtgtaacggt agtggatgac ttcgtgacgc cggataacgt agcgcaatat atgaatgccg
     1741 gttattcgta cgtgattgat gctattgata gtgtacggcc caaagcggcg ctgattgctt
     1801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgctggtggg cagattgacc
     1861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgttg gcggcgaagt
     1921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
     1981 tggattgcgt gttctctacc gaagcgctgg tgtacccgca gtcagacggc accgtctgtg
     2041 cgatgaaagc cacggcggaa gggccaaagc ggatggattg tgcctcagga tttggcgcgg
     2101 cgacgatggt gaccgccact tttggctttg ttgcggtttc tcatgcgctg aagaagatga
     2161 tggcgaaagc ggcgcgtcag gggtaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2221 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg cttaacgcat
     2281 tcaacccctg gctgcgtgag gcgctaagtt gcgcacgtaa tcccagctca tcaaacaatg
     2341 ccagcggcga ctgtgcctgc aattcggcag cggttttccc ctcaacggca gtcaacaaca
     2401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaaatgc atcttgccgt
     2461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2521 gcgcttttaa ctcctctggc aaagccggaa gctgtttccc cagcatgatc aactgccgat
     2581 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     2641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
     2701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     2761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgctg
     2821 cccggcgcgc agggcaatac cgtactccgc cagcagtgtc accatatcgc tgtgatgaac
     2881 tccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     2941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcttg
     3001 gttgatatcg taatctgtca gccattccag cgccgcgctt aatcctatga cgccagcgac
     3061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaaac cgtcaaaact
     3121 cacttcgtga accattttgc cgccgcccag ccagggcgac atcgcttcca gcagttctgg
     3181 tttgccatac agcacgccga tgcctgtcgg accatacagt ttgtgacctg aaaaagcata
     3241 gaaatcaata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3301 aaccatcacc accatcccgg ctgaatgagc aaaagtaatc gctctcgcca gatccgggca
     3361 accgccagtg acgttcgaca tctggcccaa cgccagaatc cgactacggg gagtaatcag
     3421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3481 ggcccccgtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3541 actgacaata atttcatcgc ccggttgcag acgcggacgg gcatagcatt gcgccaccat
     3601 gttgatggat tcagtggtgc cgcgcgtcca gacgatattt ttgtcatccg atgcattcag
     3661 taattgtgcc actttttccc gcgcagcttc gtaacgcgcg gtcaggcgtt gggcttcggc
     3721 aaactggctg cgatggacgt ttccggcgct caggctataa aactgtcggg aggcttcaac
     3781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     3841 cgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     3901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     3961 tatctggata tcctggcgaa gccgcgtcag taacatatgc tgaatagtgt taggcgttaa
     4021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4261 tcaaccgtac cgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtaatctaat
     4621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
     4681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga
     4741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttctggg
     4801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
     4861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
     4921 ccgtagcgct aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
     4981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgatgttc gaatccaggc
     5041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
     5101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
     5161 ctcgccgcta ctgctgactg gcgaaaaccc cttgaagacc ccggaagatc tggctaaaca
     5221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
     5281 aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg tgctgcaagc
     5341 ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaattga
     5401 agccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg ctttttatct
     5461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
     5521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
     5581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
     5641 tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg ccgttgagat
     5701 gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
     5761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
     5821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
     5881 gtgggcgttt gttacaccgg ttggcggcgt gagcttcctt gcgggttggg cgttaatgtt
     5941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
     6001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
     6061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtgatttat gaatgttatc
     6121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
     6181 gccagtggtt tgtagtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
     6241 ccattgttgg catgttgcag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
     6301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaattcacc gtgccgctac
     6361 gcgctgcctt gcgtgatgcg ggggtgctgg cgaactatga aacgccgaag cgtccggttg
     6421 tgcatgtatt ctttattgca ccaggctgtt gctataccgg ttactcatac agcaacaata
     6481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagatgcg ccgagtcgtt
     6541 ccacgctcaa actggaagag gcatttcatg tgtttattcc cgcggatgag tgggatgaac
     6601 gcctggcgaa cgggatgtgg gcggtggatt tgggcgcttg cccaggcggc tggacctacc
     6661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
     6721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
     6781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggcgaaa gttgcggcat
     6841 taatggcgca gtggctggtt aatggctggt gccgtgaaac tatcttcaat ctcaaactgc
     6901 cgatgaaaaa acgctacgaa gaagtgtcac acaatctggc gtatattcag gcacagcttg
     6961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
     7021 tgacggtgca cgtccgccgt atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
     7081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
     7141 agttgcaacc tggcgctcat cagaaataat gtgcgtgacc tcatccagat gcccgatgcg
     7201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
     7261 ttttaataac attgatttgt aatcagcgtt gatggcattg gagtcccaca gtgcgccgct
     7321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
     7381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
     7441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
     7501 ctggatgttg atgtcaggca actggcgtgc cagataccag caggttgaac tggcgtctaa
     7561 ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
     7621 cgcataatgg cttttcagcc ggatgtgaaa gggatcgcca ctgtcttgat tttgacggtg
     7681 gatatattta gcgcgcccat gattgcgcag aattttaccc tgcgtctgta attcattgag
     7741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
     7801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
     7861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
     7921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
     7981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
     8041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
     8101 accttctacc gccgccatca tcaccagcgg tggtgcataa ctgtccagtt caaataacgg
     8161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
     8221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gccccatctc
     8281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
     8341 cctcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
     8401 gcgcgcgggc ttcttccggg ctgttaaatt cccctacgcc ataccaacca aacagcgcag
     8461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
     8521 ctttaatctg attccacaat gtgttgcgac ttcctccgcc gactaacaat aattcagagg
     8581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gtggttaacc
     8641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgttacg ccttgccagc
     8701 cagcgttctg gcacgacaat aaatcacact gcattttcac tccatctgcg ccaggcgcga
     8761 tcaggcgagc ttcttcaatc aacatttgcc agggagtttc agccgtccag aacagttttc
     8821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccctgcct
     8881 ggctatccag ttcgcaggta gaaccggcgt actgacttaa cagcgaagta tcaacctggg
     8941 cgctgcggac cattaaaatt tcccatgtac cggaagagag cacgggttca ttttgttcgg
     9001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
     9061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
     9121 ccaccagacg agggaagagt cggcgtggaa tactggtggc ttgtaaaatt tgcggactga
     9181 aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtaata tcagtagtga
     9241 attcgccggt taaacggtgg ttaatcagcg acgaaataaa gagccaggcg tgcgcgcgtt
     9301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
     9361 aggctccgac tccagaaata gcctgcaact gctgtgcgga gatcaaccgt tcaatattgt
     9421 ccattaccac ggctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
     9481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgatacca cggatgtggc
     9541 aatcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcgt
     9601 ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg
     9661 aggcgcgggc aacaattttt ccctgccgat taaccgcgat ggccctgaca ttggtcgcgc
     9721 cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata tagccggata
     9781 acattgctta tccggctaac cactcttgct tgttcaggcc cggtagccgg agcgaccggg
     9841 catcacatca gggagtcatg tattaacgct tgtacaacgg gccgtagttc tggcaagcgc
     9901 ggtaatcctg gccttcaata tccatgccgt gcgcggccca ggcagaagga cgatacactt
     9961 tggtctcttc aacgttgtgc atacataccg ggatacgcag catggaggca agagtgataa
    10021 agtctgcgcc aacgtggccg atggtcagaa ctccgtggtt agcgccccag ttcgccatta
    10081 ccgagtacac atccgtaaac ggccctttac cggtgaggcg cggtgcaaac caggtggttg
    10141 gccaggttga gttggtgcgt ttgttgagga tgtcatgcac atccttcggc aattccacgc
    10201 tccagccttc cgcgatttgc agtaccggtc ccaggccttt gatgatgttg acacgagtca
    10261 tggtgaacgg gacgccgcct tcggtaagga agcgggaaga gtaaccgccg ccacggaagt
    10321 attcgtggat cgccgggcac cattcggtag cggcgaggca agcgtcagcc tcttgctgag
    10381 agatttccca gtgcggcttc atcgtcggtt taccttcgct gtcgcgttgt ttacaggagc
    10441 cgtccagcgc agcagaaccg gagttgatca aatggatgat gccgtgttct gccagcccgt
    10501 ccagtttatg ccccgttaca cgctcaattg cttctggtga ccagtaggta cggacatcgg
    10561 caaatacctg agcggtgccg gtgagctggt ggcccattaa cattgccacg ccgttaagac
    10621 tgtcgttttc ggtcgccacg acaaacggtt tgcgcacgcc attccagtca aatgaactgt
    10681 tgaggatcgc ttcagcggta tcgccattgg gatattgatc ggtccagtga cgttgcccct
    10741 ggaagcctgc agcgatggcg ttgtagccaa gtgattcttc cacgcgacca atatcggcca
    10801 gtttgctatt gccttgcatc atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca
    10861 gaactgcgcg gctttgctcg gcattgcgtt gatactgttt gttattttca tcttcgccat
    10921 agcggaagtt tttatcagcc caggccagcg ccatttccag ttcggcttcg tcgtaaatct
    10981 tctgatcgat acggcgacgc agttcggtca tatccaccgc ctggactttc attcccagcc
    11041 aggattcaaa gaagttgtga tcaacgatgg aaccggcgat acccatcgaa acgccgccca
    11101 gcgacagata acttttacct ttcatgctgg cgactgctaa accggcgcgg gcaaagcgca
    11161 gcagtttttc ttcaacatcg gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac
    11221 cgtaaatgga gaatgccggg atgcctttct ggctgtgagc tgccagagcc gctgccaggt
    11281 aaacagcgcc gggacgttca gtgccgttaa agccccaaat ggctttcggg cgggttggat
    11341 ccatgtcgat ggtttcactg ccatagcacc agcaaggcgt taccgtaatg gtgaggccta
    11401 cattctgact gctgaatttt tcttcgcaag cagcggcttc agccataccc gcgatacagg
    11461 tatcggaaat gacacactcg acggcagctc cgcaggcatg gcgcagtttc tcggtcagca
    11521 gtgcagccgt ggctttcgcc atattcattg tttgttcttc aagcgactca cgaacaccca
    11581 tgcgacgacc gtcaataacc gggcggatac caattttcgg taagctgatt tttttcatta
    11641 ctgattcctc actttattcg gaaaatatgt tcagttagtt gccgtttgag aacggaaacg
    11701 ggcaaagata aagatgactg cgaagcagag tgccgggatc agttcggcag tggggatgtt
    11761 gcccgctgcg tcactgacaa accccatgac cggagtgaca ataccgccgc caataatggt
    11821 cataacgatg aaggacgaac cgtatttggt gtcctggccg agattcttaa tgcccagcga
    11881 gaagattgtt gggtactgaa tcgacataaa ggcactgcat aaagtcaggg ctattaagcc
    11941 cacatgaccg ccagcgaagg ctgagatcag gcacagtgcc atagcgatta atgcgtaggc
    12001 tgccaggact ttgtgtggcg cgaagcgact gatgagccag gtaccggtga aacgaccaat
    12061 aaagaagcac accatggttc cggttaaata gttagcggca aagcctgctg tcatacctgg
    12121 aatttcttct acagcgtagc gaattaaata gctccagcag gccgtttgtg cgccaacgta
    12181 gcagaattgc gctaatactg cccagcgcca gtggcgaata cgcgccaggc gagaaagcga
    12241 tgcggagaac gatccttgtt tggcgtcact gtgattatca ctctgcaatg ccgggaattt
    12301 cgtcagcatg atcagcaggg cgaccagtaa cacgatagcc acgatgatca tataaggtgt
    12361 ctgtaccgat aataccaagc tgtgtttata cgcactcaat tgctctggag acattttatc
    12421 gagaacgtct tgcgattgat gtggcacgtt agacaaaata agactttgcc caaagacaac
    12481 cgcgataatt gcgccaaatg agttaaatgt ttgcgcaaga tttaagcgga agtgaccact
    12541 gctttccggc cctaataccg taacaaaagg gtttgcggca gtttccagac aacctaatcc
    12601 ggctgcaata ataaataggc caactaaaaa caaggtgtag ttcattattt ctgcggcggg
    12661 ccagaataat gcagcgccga aggcatataa aaacaatccg gtaataatcc ctgctttata
    12721 actgagtttt ttcatcaata tcccggcagg gattgggata atgaaataac caaagtaaaa
    12781 ggccgattgg atcaggccag cctggaaatt tgtcagcgta aaagcctgct ggaattgagg
    12841 caataaaatg tcgttaaggt tattggctac cgcccaaaga aaaaacagtg agcacagcag
    12901 cgcgaatgga ataatgtaac ttctgctttg ccctgcatct ttatctaccg cacggtaact
    12961 ctgcgtttgt attgatgtgt ttcccatagc atcctcttag gttcagaagc ttaagcacgt
    13021 aatcggttat gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat cagtgaatat
    13081 ttgcctgtct gaaatgaact catggattca ttttcaaata aaaaacgctc tgtaatatga
    13141 cggcggtcac acttattagc cggagggtga ctttatgtga ctaccatcac tttaataatc
    13201 gaaatttcat gcccgtttca gaaaataaat cataaaaaac acaaaccaga tgaccgtatt
    13261 aattgtttgt gccgaaaacg ggcattcctg gca
//
LOCUS       DACADA010000124        13210 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.126, whole genome shotgun sequence.
ACCESSION   DACADA010000124 DACADA010000000
VERSION     DACADA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.126"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            282..617
                     /locus_tag="ISB17_003528"
     CDS             282..617
                     /locus_tag="ISB17_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAP1981419.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            777..1220
                     /gene="yjdN"
                     /locus_tag="ISB17_003529"
     CDS             777..1220
                     /gene="yjdN"
                     /locus_tag="ISB17_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAP1981420.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1353..2141
                     /gene="phnC"
                     /locus_tag="ISB17_003530"
     CDS             1353..2141
                     /gene="phnC"
                     /locus_tag="ISB17_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP1981421.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2166..3182
                     /gene="phnD"
                     /locus_tag="ISB17_003531"
     CDS             2166..3182
                     /gene="phnD"
                     /locus_tag="ISB17_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1981422.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3287..4066
                     /gene="phnE"
                     /locus_tag="ISB17_003532"
     CDS             3287..4066
                     /gene="phnE"
                     /locus_tag="ISB17_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP1981423.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4087..4812
                     /gene="phnF"
                     /locus_tag="ISB17_003533"
     CDS             4087..4812
                     /gene="phnF"
                     /locus_tag="ISB17_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAP1981424.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4813..5265
                     /gene="phnG"
                     /locus_tag="ISB17_003534"
     CDS             4813..5265
                     /gene="phnG"
                     /locus_tag="ISB17_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAP1981425.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5262..5846
                     /gene="phnH"
                     /locus_tag="ISB17_003535"
     CDS             5262..5846
                     /gene="phnH"
                     /locus_tag="ISB17_003535"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAP1981426.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5846..6910
                     /gene="phnI"
                     /locus_tag="ISB17_003536"
     CDS             5846..6910
                     /gene="phnI"
                     /locus_tag="ISB17_003536"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAP1981427.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6903..7748
                     /gene="phnJ"
                     /locus_tag="ISB17_003537"
     CDS             6903..7748
                     /gene="phnJ"
                     /locus_tag="ISB17_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAP1981428.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7745..8503
                     /gene="phnK"
                     /locus_tag="ISB17_003538"
     CDS             7745..8503
                     /gene="phnK"
                     /locus_tag="ISB17_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAP1981429.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8614..9294
                     /gene="phnL"
                     /locus_tag="ISB17_003539"
     CDS             8614..9294
                     /gene="phnL"
                     /locus_tag="ISB17_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAP1981430.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9291..10427
                     /gene="phnM"
                     /locus_tag="ISB17_003540"
     CDS             9291..10427
                     /gene="phnM"
                     /locus_tag="ISB17_003540"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAP1981431.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGKIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVEREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10427..10984
                     /gene="phnN"
                     /locus_tag="ISB17_003541"
     CDS             10427..10984
                     /gene="phnN"
                     /locus_tag="ISB17_003541"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAP1981432.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10971..11405
                     /gene="phnO"
                     /locus_tag="ISB17_003542"
     CDS             10971..11405
                     /gene="phnO"
                     /locus_tag="ISB17_003542"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAP1981433.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11407..12165
                     /gene="phnP"
                     /locus_tag="ISB17_003543"
     CDS             11407..12165
                     /gene="phnP"
                     /locus_tag="ISB17_003543"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAP1981434.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12193..12522)
                     /gene="yjdP"
                     /locus_tag="ISB17_003544"
     CDS             complement(12193..12522)
                     /gene="yjdP"
                     /locus_tag="ISB17_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAP1981435.1"
                     /translation="MKRFPLFLLFTLFTLFTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQERQQLEDEERR
                     MEDEYGR"
     gene            complement(12591..13040)
                     /gene="rpiB"
                     /locus_tag="ISB17_003545"
     CDS             complement(12591..13040)
                     /gene="rpiB"
                     /locus_tag="ISB17_003545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HAP1981436.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2988 a         3926 c         3622 g         2674 t
ORIGIN      
        1 attagaataa ataacatctc ccaatacgtt taaaacgtca gaaagataaa aacatcatgt
       61 gaattaaaaa aagaacgagt agagcattaa ccttatctta aataataaat ggaggcaaaa
      121 agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcatcta aaatcagcag
      181 tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc aatctgccgt
      241 tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta ccacactgcc
      301 caaaatgcaa ctccgaatac acctacgaag ataacggcat gtacatctgc ccggaatgtg
      361 cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc gttaaagatg
      421 ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat ctgaaagtga
      481 aaggtagctc ttcgatgctg aaaattggca ccaaagtaaa aaacatccgc ctggttgaag
      541 gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg aagtctgagt
      601 ttgtgaaaaa gaactgattg tattgtgatc ggtaggccgg ataaggcgct cgcgccgcat
      661 ccggcaacgg tggcgactgc ctgatgcgac gctgaccgcg tcttatcagg cttccctctc
      721 atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc accatcatgc
      781 cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc tattatcaac
      841 gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgcca aaatcaacgc
      901 aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc atcgcccacg
      961 ccaatgtgcg cattgcagga agcgacatca tgatgagcga tgctataccg tcaggaaaag
     1021 ccagctactc cggctttacg ctggtgctcg attctcaaca ggtggaagaa ggaaaacgct
     1081 ggtttgataa ccttgccgcc aacggaaaaa tcgaaatggc ctggcaggaa acgttctggg
     1141 cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcatca
     1201 aacaacagcc aacgcaataa cccgccggga ggcccgccct cccgcactgt catcgaattc
     1261 ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa taatccgcca
     1321 cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt gtcgagaagc
     1381 tcgccaaaac cttcaatcag catcaggcgc tgcacgcggt tgatctgaac attcatcatg
     1441 gtgaaatggt ggctctgctt gggccgtcgg gttccggaaa atccaccctt ttacgtcact
     1501 taagcggttt gattaccggc gataaatctg tcggtagcca tatcgagctg ctgggccgca
     1561 cagtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc aacaccggct
     1621 acatctttca acaattcaac ctggtgaatc gcctgagcgt actggagaac gtgctgattg
     1681 gcgcgctcgg cagcacgcca ttctggcgaa cctgttttag ctacttcacc cgcgagcaga
     1741 aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcactttgcc catcaacgcg
     1801 tttccacact ctccggcggc cagcaacagc gtgtggcgat tgcccgcgcg ctgatgcagc
     1861 aggcgaaggt cattctcgct gacgaaccca tcgcctcgct ggacccggaa tcggcacgca
     1921 tcgtgatgga caccctgcgc gacatcaacc agaacgacgg cattaccgtg gtcgtcacgc
     1981 tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc
     2041 acgtcttcta cgacggctgc agccaacagt ttgataacga acgttttgac catctctacc
     2101 gcagcattaa ccgcgtcgaa gagaacgcga aagctgcctg acatccccat cattgaggaa
     2161 aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca
     2221 ccctgttaaa tccggcatac gccgaagagc aggaaaaggc gctgaatttc ggcattattt
     2281 caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gttcttacag gatatggaga
     2341 agaagctggg cgtaaaggtc aacgccttct ttgccccgga ctacgcgggc attatccagg
     2401 ggatgcgctt caataaagtg gatatcgcct ggtacggcaa cctgtcggcg atggaagcgg
     2461 tggatcgcgc caacggtcag gtcttcgccc agacggtcgc ggcggatgga tcgccgggtt
     2521 actggagcgt gttgatcgtc aacaaagata gcccgatcaa caacctgaac gatctgctgg
     2581 cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctctacctcc ggcttcctcg
     2641 tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttt aagcgcaccg
     2701 tcaacgccgg gcatgaaacc aacgccctcg ccgtcgctaa caagcaagtg gatgtcgcca
     2761 ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag ctgaaagaac
     2821 tgaaggtgat ctggaaatca ccgctgatcc ctggcgatcc gatcgtctgg cgcaaaaatc
     2881 tttccgaaac cactaaagac aagatctacg acttctttat gaattacggc aaaacgccgg
     2941 aagagaaagc ggtgctggag cgcctgggct gggcgccgtt ccgcgcctcc agcgacttgc
     3001 aactggtgcc gattcgccag ctcgcactgt ttaaagagat gcaaagcgtg aaggacaaca
     3061 aagggctgaa cgaacaggac aagctggcaa aaaccaccgc cattcaggcg caactggatg
     3121 atttggaccg cctgaacaac gcgttaagcg caatgagttc ggtgagtaaa gcggtgcagt
     3181 aaatcgtagg tcggataaga cgcccggcgt cgcatccgac aatgtgcaga cgttgatgcc
     3241 ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc aaaccatcac
     3301 catcgcccca cccaagcgca gctggttctc gcttctgagc tgggccgttg ttatcgccgt
     3361 gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta aagatggcgg
     3421 caacatggcg acgttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta
     3481 cctcaccgaa atggccgtca cgctgcaaat cgccgtctgg ggcaccgcgc tggcggtggt
     3541 tctctccatc cccttcggcc tgatgagcgc tgaaaacctg gtaccgtggt gggtctacca
     3601 acctgtacgc cgcctgatgg acgcctgccg cgccattaac gaaatggtct tcgccatgct
     3661 gttcgtggtc gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt ttatccacac
     3721 caccggcgtg ctctccaagc tgctttccga agcggtggaa gcgatcgaac ccggcccggt
     3781 ggagggcatt cgcgccaccg gtgccaacaa gctggaagag atcctttacg gcgtactacc
     3841 gcaggtcatg ccgctgctga tttcctactc tctctatcgc ttcgaatcca acgtccgctc
     3901 ggcgaccgtc gtcggtatgg ttggcgcggg cgggatcggc gtcacgctgt gggaagcgat
     3961 tcgcggtttc cagttccagc aaacctgcgc cctgatggtg ctcatcatcg tcacggtcag
     4021 cctgctggat ttcctctctc aacggttgcg taagcacttt atctgataag cgaggcattg
     4081 atatctatgc acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc
     4141 gcaaaacttg agcaggagct tcgtcaacac taccgctgcg gcgactatct tcccgccgag
     4201 cagcaactgg cggcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc catcgaccaa
     4261 ctggtggaaa aaggctgggt acagcgccgt cagggcgtcg gcgtactggt gctgatgcgc
     4321 ccgttcgatt acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggc
     4381 agccatccca ccagcgaaaa attgctatcg gtgctgcgcc ccgcctccgg tcacgtcgct
     4441 gacgcgctgg ggataaccga gggggagaac gtcatccacc tgcgcaccct gcgtcgggtc
     4501 aacggcgtcg cgctctgttt aatcgaccac tactttgcgg acctcaccct ctggccgacg
     4561 ctgcaacgtt tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac ggggattacg
     4621 ctacgacgca gtcagacgcg gatcagcgcc cgtcgcgccc aggccaaaga gtgccagcgt
     4681 cttgaaatcc cgaatctgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt
     4741 gaaagcagcc cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact
     4801 atggagcact gaatgcacgc agataccgcg acccgccagc actggatgtc cgtgctggcg
     4861 cacagccaac cggctgaact ggcagcccgc ctgaacgcgc tgaacatcaa cgccgattat
     4921 gaggtgatcc gcgccgctga aactggcctg gtgcagattc aggcgcgaat gggcggcacc
     4981 ggcgaacgct tttttgctgg cgacgccacg ctcacccgcg ccgccgtgcg tctgactgac
     5041 ggcacgctcg gctatagctg ggtgctgggg cgcgacaaac agcacgccga acgttgtgcg
     5101 ctgattgatg cgctgatgca gcaaaaccgc cactttcaaa acttatcaga aacccttatc
     5161 gccccgctgg acgctgaccg tatggcacgc attgccgcgc gccaggccga agtgaacgcc
     5221 agccgggtcg acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt
     5281 ttatgcttcc cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg
     5341 agccgggcgt gattgtcgcc ctgcatcagc tcaagcgcgg ctggcagccg ctgaatatcg
     5401 ccaccaccag cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccgcgc
     5461 cattgagcaa cgatatcgtc aatcagagcc tgcgttttca taccaacgcg tcgctggtta
     5521 atcagccgga acaggcgacc ttcgcggtaa cagatgaggc gatctccagc gagcagctca
     5581 acgccctttc caccggcact gccgttgcac cagaagcggg cgcaacgctg attttacagg
     5641 tcaccagcct gagcggcggg cgtatgttgc gcctcaccgg cgcaggtatt gccgaagaac
     5701 ggatgatcgc cccgcaactg ccaaagtgca ttcttcatga acttaccgag cgcccgcacc
     5761 cgttcccgct cggcatcgac ctgatcctga cctgcggcga gcgcctgctg gctattccgc
     5821 gaaccactca tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg aaaaggcgat
     5881 cgacgccgcc cacgccctgc aagagagccg acgccggggc gatactgatt tgcctgaact
     5941 gagcgtcgcc cagattgaac agcagcttaa cctcgcggta gatcgcgtga tgaccgaagg
     6001 cggtattgcc gaccgcgagc tggccgcgct ggcgctgaaa caggccagcg gcgataacgt
     6061 cgaagcgatt tttctgttgc gcgcctaccg caccacgctg gcgaagctgg cggtcagtga
     6121 gccgctcgac accaccggga tgcgtctcga acggcgtatt tcagcaattt ataaagacat
     6181 tcccggcggc caactgcttg gccccaccta cgactacacc catcgcctgc tcgatttcac
     6241 cctgctggca aacggcgaaa cgccaacgct gaccaccgct gacagcgaac agcaaccgtc
     6301 gccacacgtc ttcagcctgc tggcgcgtca ggggctggcg aagtttgaag aagatagcgg
     6361 cgcacaaccg gacgacatca cccgcacgcc gccggtttat ccctgttcgc gttcttcccg
     6421 tctacaacaa ttaatgcgcg gtgacgaagg ctatttgttg gcgctggcct actccaccca
     6481 acgcggttac gggcgcaatc acccgttcgc aggcgaaatt cgcagtggct atatcgatat
     6541 atttattgtg ccggaagagc tgggatttgc ggtgaacgtc ggcgaattgt tgatgaccga
     6601 gtgtgaaatg gtcaacggtt ttatcgaccc gccggatgaa ccaccgcatt ttacacgcgg
     6661 ctatgggctg gtgttcggca tgagcgagcg caaagcaatg gcgatggcgc tggttgaccg
     6721 tgcgttgcag gcaccggaat acggcgagca cgcaacaggt ccggcacagg atgaagagtt
     6781 cgtgctggcc catgccgaca acgtcgaagc cgcaggcttt gtttcgcatc tcaaactccc
     6841 gcactacgtc gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagcagaa
     6901 ccatggctaa tctgagcggc tacaactttg cttacctcga cgagcagacc aaacgcatga
     6961 tccgccgcgc tatcttaaaa gcggtggcaa tccccggtta tcaggtgccg tttggcggac
     7021 gcgagatgcc gatgccctac ggctggggaa ccggcggcat tcagctcacc gccagcgtga
     7081 ttggcgaaag cgacgtgttg aaggtgattg accagggcgc agacgacacc accaacgccg
     7141 tgtcgattcg taacttcttt aagcgcgtga ccggggtgaa caccaccgag cgcacggacg
     7201 atgcgacact tatccagacg cgtcaccgca tacccgaaac gccgctgacc gaagatcaga
     7261 tagttatctt ccaggtgccg atccccgaac cgctgcgctt tattgagccg cgtgagacgg
     7321 aaacgcgcac catgcacgcg ctggaggagt acggcgtgat gcaggtgaag ctgtatgaag
     7381 atatcgcccg cttcggtcat atcgccacca cctacgccta tccggtgaag gtgaacgggc
     7441 gctacgtgat ggacccgtcg ccgatcccga aattcgataa cccgaaaatg gacatgatgc
     7501 ccgccctgca actgtttggc gcggggcgcg agaagcgcat ctatgccgtg ccaccgttta
     7561 cccgcgtgga aagcctcgat ttcgatgatc atccgttcac cgtccagcag tgggatgaac
     7621 catgcgccat ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg
     7681 gaaaccgcat gtttgtctgc tccgataccg attattgccg ccaacagagc gaggccaaaa
     7741 gccaatgaat caaccgttac tttcggtcaa taacctgacc cacctttatg ccccgggcaa
     7801 aggcttcagc gatgtctctt ttgatttatg gccgggagaa gttctgggca ttgttgggga
     7861 gtccggttcc gggaagacca cgctactgaa atcaatttcc gcccgcctga caccacagca
     7921 aggggaaatt cgctacgaga accgttcgct gtacgcgatg agcgaggccg accgccgtcg
     7981 cctgctgcgc accgaatggg gtgtggtgca tcagcatccg cttgacggcc tgcgccgcca
     8041 ggtgtcggca ggcggcaata tcggcgaacg gttgatggca accggagcgc gtcattacgg
     8101 cgatattcgc gccaccgcgc agaaatggct ggaagaggtg gagattcccg ccaaccggat
     8161 cgacgacctg ccgaccacct tttccggcgg catgcagcaa cgtttgcaga ttgcccgcaa
     8221 cctggtgacg catccgaagc tggtgtttat ggatgaaccc actggcgggc tggatgtgtc
     8281 ggtacaggcg cgcctgctgg acctgctccg cggtctggtg atggagctta acctggcggt
     8341 agtgattgtc acccacgatt taggcgtcgc ccgtctgctg gcggaccgtt tgctggtaat
     8401 gaagcaaggg aaagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcacca
     8461 tccgtatacc cagttgctgg tgtcatcggt tttgcagaat tgagctggtg ccggatgcgg
     8521 cgtaaacgcc ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcatcagc
     8581 gtcgcatccg acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa
     8641 accttcatcc tgcaccagca aaacggcgtg cgcctgcctg tattacaagg cgcctcactc
     8701 accgtcaacg ccggcgaatg cgtggtactc cacggccatt ccggcagtgg aaaatcaact
     8761 ctgctacgct cgctgtatgc caactatcta cccgacgaag ggcaaatcca gatcaaacac
     8821 ggtgatgaat gggtcgacct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa
     8881 accactgtcg gctgggtaag ccagtttctg cgcgtcatcc cacgtatttc ggcactggaa
     8941 gtggtgatgc agccgctgct cgataccggc gtaccgcgtg aagcctgcgc cgctaaagcc
     9001 gcgcgtctcc tcacccgcct gaacgtgcct gaacgcctgt ggcaccttgc gccatcgacc
     9061 ttctccggtg gcgaacagca gcgtgtcaac atcgcccgtg gctttatcgt cgactacccc
     9121 attctgctgc tcgacgaacc taccgcctcg ctggacgcta aaaacagcgc cgccgtagtg
     9181 gaactgatcc gcgaagccaa agcccgcggc gcggccatcg tcggtatctt ccatgacgaa
     9241 accgtacgcg agcaagtcgc cgaccgcctg cacccaatgg gagtctcttc atgattatca
     9301 ataacgtcaa actggtgctg gaaaacgagg tggtacacgg ttctctggag atgcaggacg
     9361 gcaaaatccg cgcctttgcc gaaagccaga gtcacctgtc agaagcaatg gacggcgaag
     9421 gcggctggct gctaccgggg ctgattgagc tgcataccga taatctggat aaattcttca
     9481 ccccgcgccc gaaagttgac tggcctgccc actcggcgat gagcagccac gacgcgctga
     9541 tggtggcgag cggcatcacc accgtgctgg acgccgtggc gattggtgat gtacgcgacg
     9601 gcggcgatcg gctggagaat ctggagaaga tgatcaacgc catcgaagag acgcagaaac
     9661 gcggcgtcaa ccgtgccgag caccgtctgc acctacgctg cgaactgccg catcacacca
     9721 cgctgccgct gtttgaaaaa ctggtcgagc gagaacccgt gacgctggtg tcgctgatgg
     9781 atcactcacc gggccagcgc cagttcgcca accgcgaaaa atatcgtgaa tattatcagg
     9841 gcaaatactc gctcaccgat gcgcagatgc aacagtacga agaggagcaa ctggcgctcg
     9901 ccgcacgctg gtcacagccg aatcgcgaat ccatcgccgc aatgtgccgc gcgcgacaca
     9961 ttgcgctcgc cagccacgat gacgccacgc acgcccacgt agcccaatcc catcagcttg
    10021 gcagcgtgat cgccgaattt cctaccacat tcgaagcggc agaagcctcg cgcaagcacg
    10081 gcatgaacgt actgatgggc gcgccgaata tcgtgcgcgg cggttcgcac tccggcaacg
    10141 tggcggccag tgaactggcg cagcttggcc tgctggatat cctctcttcc gactactacc
    10201 ccgccagcct gctggatgcg gcgtttcgcg tggccgatga ccagagcaac cgctttacgc
    10261 taccgcaggc ggtgaggctg gtgaccaaaa acccggccca ggcactgaat ctccaggatc
    10321 gcggggtgat tggcgagggc aaacgcgccg acctggtgct ggcgcatcgc aagggcaatc
    10381 acattcatat tgaccacgtc tggcgtcagg gtaaaagggt gttctgatga cgggaaaact
    10441 gatttggtta atgggtccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcca
    10501 gcgggaacaa acccagctac tggtagcgca tcgctacatc acgcgtgatg ccagcgccgg
    10561 aagtgaaaat catatcgcct taagcgagcg ggagtttttt acccgcgcgg ggcaaaatct
    10621 gttggccttg agctggcatg ctaatggttt gtattacggt gtcggcattg agatcgacct
    10681 ctggctgcac gccggattcg acgtcgtggt taacggctca cgcgcccacc tgccgcaggc
    10741 gcgggcacgt tatcaatcgg cactgctgcc catctgttta caggtttcgc cggagatcct
    10801 gcgccagcgc ctggaaaacc gtggccgcga aaacgccagt gaaattaacg cccggctggc
    10861 gcgcgccgcc cgctatactc ctcaggattg tcttacgctc aataatgacg gcagcctgcg
    10921 ccagtcggtc gacaagctgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt
    10981 gtgagcttcg cccggctacg cagtacgaca ccgacgcagt ttacgcgctg atttgtgagc
    11041 taaaacaggc ggagtttgac caccacgcgt ttcgcgtggg gtttaacgcc aatctgcgcg
    11101 atccgaacat gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc
    11161 tgcatttgca gtttcatctg catcatgtca actggatcgg cgaaattcag gagttggtgg
    11221 tgatgccgca ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag
    11281 aagcccgcca ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg
    11341 cgcaccgttt ctatctgcgc gaaggctacg agcagagcca tttccgcttc accaaagcgc
    11401 tgtaacatga gcctgatcct cacgctcacc ggcactggcg gcgcacaggg cgttcccgca
    11461 tggggctgtg agtgcgcggc ctgcgccaga gcgcggcgct caccgcagta tcgccgccag
    11521 ccatgcagcg gcgtcgtgaa gtttaacgac gcgatcaccc tgatcgacgc cgggctgcac
    11581 gatctcgccg atcgctggtc gcccggatcg ttccagcagt ttttgctgac gcactaccat
    11641 atggatcacg tgcaggggct gtttccgctg cgctggggcg tgggcgacac aatcccggtc
    11701 tacggtccac cggatgagca gggctgcgac gatctgttta aacatccggg cctgcttgat
    11761 ttcagccaca cggtggagcc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg
    11821 ctgccgctta accattcgaa actcactttc ggctatttac tggaaacggc gcacagccgg
    11881 gtggcgtggc tatctgacac cgcaggcctg ccggaaaaaa cgctgaaatt tttacgcaat
    11941 aatcagccgc aggtaatggt gattgattgc agccacccgc cgcgcgagga tgcaccgcgt
    12001 aatcactgtg atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacgggtg
    12061 attctgaccc atatcagcca ccagtttgat gcgtggctga tggaaaatgc actgccgtcg
    12121 gggtttgagg cggggtttga tgggatggag attgaggtgg agtgagggaa aatacccgcg
    12181 ccggccccta cctcaacgcc catactcatc ttccattctc cgctcttcat cttccagttg
    12241 ctgacgctcc tgatcaagct ggcgctggcg ttcgtccagc tgcctgcggc gatcttcaaa
    12301 ctggcggcgg cggtcatcat attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc
    12361 gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt cgttaatcgc
    12421 ctgctgaata ttgccaatgg tgtcatcgat aatatcggcc tgggccggaa cggtgaacag
    12481 cgtgaacagc gtgaataaaa gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga
    12541 aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc tcaatttctc
    12601 cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt
    12661 gcgcccagcc acgcatcgac aatcattttt gccagttcga ggccaactac tcgtgaacca
    12721 aaagccagca cgttggtgtc gttatgctgc cgcgaaagtt gcgcggaata aggttcgcta
    12781 caaacgaccg cgcgaattcc ggcaaacttg ttcgccgcta tcgaaatacc gacgccagtg
    12841 ccacaaatca aaatcccgcc atcagcctct ccgccagcaa cggccagtgc gacttgactg
    12901 gcgtaatgcg gataatcagt acgctctgac gaccaggttc ctttatcaat cacttcaacg
    12961 ccacgctcaa ctaaatgtgc cactatttca tgttttaaaa tgaaaccgac atgatcacag
    13021 ccaaatgcaa tctttttcat tcatcttccc cgctcgcgca aaacttcaca ataaaaaaac
    13081 atttgtgatt taaatgaatt aaaaacaccc aaaatcgatg aaatcagcct tcccgatctg
    13141 ttttacagag ttacatctgg cagcgcaata gcgttaaaga cactcactat ccggcaacat
    13201 ctatcatcta
//
LOCUS       DACADA010000125        13155 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.127, whole genome shotgun sequence.
ACCESSION   DACADA010000125 DACADA010000000
VERSION     DACADA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.127"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            449..1138
                     /gene="mutH"
                     /locus_tag="ISB17_003546"
     CDS             449..1138
                     /gene="mutH"
                     /locus_tag="ISB17_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAP1981437.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1207..1920
                     /locus_tag="ISB17_003547"
     CDS             1207..1920
                     /locus_tag="ISB17_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1981438.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2058..2276
                     /gene="ygdR"
                     /locus_tag="ISB17_003548"
     CDS             2058..2276
                     /gene="ygdR"
                     /locus_tag="ISB17_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAP1981439.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2384..3424
                     /locus_tag="ISB17_003549"
     CDS             2384..3424
                     /locus_tag="ISB17_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAP1981440.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3456..4649)
                     /gene="lplT"
                     /locus_tag="ISB17_003550"
     CDS             complement(3456..4649)
                     /gene="lplT"
                     /locus_tag="ISB17_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAP1981441.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4642..6801)
                     /locus_tag="ISB17_003551"
     CDS             complement(4642..6801)
                     /locus_tag="ISB17_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-acylglycerophosphoethanolamine
                     acyltransferase/acyl-ACP synthetase"
                     /protein_id="HAP1981442.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7387..8418
                     /gene="galR"
                     /locus_tag="ISB17_003552"
     CDS             7387..8418
                     /gene="galR"
                     /locus_tag="ISB17_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAP1981443.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8425..9687)
                     /gene="lysA"
                     /locus_tag="ISB17_003553"
     CDS             complement(8425..9687)
                     /gene="lysA"
                     /locus_tag="ISB17_003553"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP1981444.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9809..10744
                     /gene="lysR"
                     /locus_tag="ISB17_003554"
     CDS             9809..10744
                     /gene="lysR"
                     /locus_tag="ISB17_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAP1981445.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10731..11423)
                     /gene="ygeA"
                     /locus_tag="ISB17_003555"
     CDS             complement(10731..11423)
                     /gene="ygeA"
                     /locus_tag="ISB17_003555"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAP1981446.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11552..12970)
                     /locus_tag="ISB17_003556"
     CDS             complement(11552..12970)
                     /locus_tag="ISB17_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP1981447.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3359 a         3520 c         3219 g         3057 t
ORIGIN      
        1 agatatagag attttaaaat attcattatc agtcaatgta ttacatgtga tttgagtaat
       61 aaaaaagagt aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa
      121 ccaacgccgt atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact
      181 ggagtttcgt cgcacgtcaa ggctgtaaat ggaaacagta gtgaaggttt ttcacagtta
      241 tcccagcttt ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg
      301 gaaaacccgt tgcagtgttg cgcaactcga ttaccggcaa cctaaaaagc aatataaatc
      361 agagaattga acaacgcatg tggaaaaagt tacactgcga atattcggca cataattgct
      421 gtttgttttt taaccaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc
      481 cgaaactgaa gaacaattgt tagcgcaagc acaacaactt tctggttata cattgggaga
      541 actggcggca cttgccgggc tggttacgcc ggagaattta aaacgcgata agggctggat
      601 tggcgtgtta ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt
      661 tgctgctctg ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga
      721 aacaacattc gtttgtgttg ccccgttaac gggcaatagc ggggtgacat gggaaaccag
      781 ccacgtgcgc cacaagctca aacgcgtgct gtggataccg gttgaaggcg agcgcagcat
      841 cccgctggca aagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga
      901 ccggcagcta cgcgaagact gggaagaatt aatggatatg attgttctcg gtcagattga
      961 gcggatcacc gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa
     1021 agcgctgacc gaagctattg gtgcccgggg cgaacggatt ctgacgctgc cgcgcggatt
     1081 ttatttgaag aagaatttca ccagtgcgct actggcccgt cattttctga tccagtagcc
     1141 atcgctttga cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct
     1201 ttttagatgt tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg
     1261 acgctgctgg agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca
     1321 aagctcccaa cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtc
     1381 atgcgtctgg cgctgctggc atccatcgcc tgggttacgc gcctgacgaa tccgcttttt
     1441 acaatattca gtcaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc
     1501 cttatctgga aagccagcaa ggaaatccac gaatccatcg aaggtgaaga agaagggctg
     1561 aaaacacgcg tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc
     1621 ttcagcctcg actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg
     1681 gctgccgtgg tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt
     1741 gtcgagcgcc atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt
     1801 accctgattc tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg
     1861 ttcttctcta ttgcggttga aagcctaaac ctgattcgca acaaaaagaa tccgctctga
     1921 tactccgtac gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga
     1981 ttttcctgct ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc
     2041 caaatgagga taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt
     2101 ttgctgtttc cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt
     2161 tgaccgatgg aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc
     2221 aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa
     2281 taaggtcagc atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc
     2341 atttttatat tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat
     2401 accccacagt tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca
     2461 gaacagcgaa gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct
     2521 tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc gaaactcaag ggctaaccga
     2581 aacctacgtc ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc
     2641 caaagtgagc ggaccgtcgc gcaataatga caaaggcatc cgcccggatc aggcgctgga
     2701 tcggaagaat atccgtgaag cgctgcatga cagcctcaag cgtctgcaga ctgattacct
     2761 cgatctctat caggtgcact ggccgcagcg cccaaccaac tgcttcggca aactcggtta
     2821 tagctggaca gattctgcgc ctgcggtttc gctgctggat acgctggacg cactggcaga
     2881 gtaccaacgc gcgggaaaaa ttcgttatat aggcgtgtcg aacgaaactg catttggcgt
     2941 aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa
     3001 cccctacagc ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga
     3061 aggggtcgaa ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct
     3121 caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcgcttca cccgctatag
     3181 cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatagcct
     3241 ggaccctgct cagatggcgc tcgcatttgt acgccgtcaa ccgtttgttg ccagcactct
     3301 gctgggcgca accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt
     3361 gagcgaagac gtattagctg aaattgaagc ggtgcatcag gtttatacgt atcctgcacc
     3421 ataagattaa cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac
     3481 agcgccgtta ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg cacgaccggg
     3541 atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg
     3601 ccaaggtttt gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt
     3661 agcaacgcat tgagcggaac gacaaaaaaa ccccccagca cgccaatcag catcaacaaa
     3721 gcataggctg gcagcagctc gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa
     3781 atcccggctg gcatacagcg tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct
     3841 gcaccaacca cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg
     3901 gtaataccca gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct
     3961 ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgcg ccatagcgag
     4021 gtgcaggcat tcaggaaact gcgggtcatg ctgatgagat tccaggactg ccccggacgc
     4081 gccgccgcca gtttgggaat gtagatattg gcaatgaccg cgccaccgta ggccagtgcg
     4141 catgcggcca gggcgatcag gacatgccag tcagccagaa caccaccagc tacggaaccg
     4201 agcaaaatcg ccgctatggt agacgcctcc attaaaccgt tagcttttac taacttacta
     4261 cccgtggtta attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca
     4321 cccaccaacg tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagt
     4381 agcttcaggc cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgc
     4441 ccgacaaacg gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg
     4501 ctccactccg gatagaactg cgctttcagt aacgccagag tagcaaacag tagcgcatta
     4561 tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac
     4621 gaagtgttag tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt
     4681 caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gatagcgaat
     4741 atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt gtaacttatc
     4801 gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct
     4861 cttaatcgca gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat
     4921 ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg
     4981 cacaaagccc tgctcatcaa aacgcacgat atcgccagtg tcataccagc cgcgctccat
     5041 ttcgccgcga atattctcgg cggtgggcac ttccagtaca ccaggcttct ccacccgcag
     5101 atagccgttc attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg
     5161 aaccgacaac aggcgcgcat ccattcctgg tagaatacgc cctaccgtac cgggtttcgc
     5221 cgccatcggt acgttgatag aaacgacagg cgcgcattcg gtcacaccgt agccttcaag
     5281 gatgcgcagg ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc
     5341 gcctgccacc acatagcgta gacgatagaa gtcatacggg ttggcaaagc gcgcgtagtg
     5401 accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg cggtcataca ccaactccgg
     5461 cacaatgcgg taatgcagcg ggcttggata aaggaacact tctgcacctg tgagcagtgg
     5521 cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaagcg
     5581 atcgttggtg gtgaagtcgg caatcgtttt aatctgctca acgttcgcca gaatgctttt
     5641 atggctatgg acgacgcctt tcggatggcc ttcagaaccg gaggtaaaga ggatcagcgc
     5701 ttcttcttcc ggctgctgtt ttaactgtgc cagacgcggc atcagcaaat gagcgaaaat
     5761 ccacactttg tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac
     5821 ctgagtaagt tgctccggca gatgccagag tttgccttta tcgagaaact gacgggaagt
     5881 gaagatggtt ttgatttcag ccgccgtaat agcactggtc agccctttta ccccggcggt
     5941 gtagttcatc attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact
     6001 gatgcctgca ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag
     6061 gatgcgtcca acaaacagcg ttttcgtaag caatttgcga taggagtctg gggtaaagtt
     6121 gacgtcttcg acacatttct tcccggctcc gaagcggtac attgcgctca gtaaagattc
     6181 gtacagcgtt tcacgcgggc gcaccgccat acgcgcctcc atcattattt gatgcagcat
     6241 ttcgccagcg attttgcgac ggtcacgggc acgcggcgca tccggcatct ccacctgcgt
     6301 tggtggcaaa atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag
     6361 gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga
     6421 cttcgccgcg acaaatcccg cgccatcgta gattttcatc agcgagccag tggtggtgat
     6481 gcgtccttca gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt
     6541 aatcgccata ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg
     6601 catataccac tgttggctta ttgaggtgta aacggcaaat accggacgta cgggtaaaaa
     6661 caatgccagc agtatgccat caataaaaga gacgtgatta ggcgtgatta gaacgcgctc
     6721 gcccttcagt gccttggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt
     6781 tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga
     6841 gggagattac acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg
     6901 cgcatccgcg caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc
     6961 aatcaatacc tctgggatct tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa
     7021 cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt
     7081 gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac
     7141 ccgaagttga cttcacctat caatacctct gggatcacca ctttagcaac ctgaagccaa
     7201 acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct
     7261 ctttttttgt cttacttatt tgcgtttggc tcacattccc acaatgaaaa cacgccgccc
     7321 cttgaaccaa cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac ccactaaggt
     7381 attttcatgg cgaccataaa ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt
     7441 tcccgcgtca ttaataattc acccaaagcc agcgaagctt cccgactggc tgtgcatagt
     7501 gcaatggagt ctcttagcta tcacccgaac gccaacgccc gtgcgctggc gcagcagacc
     7561 actgaaacga tcggtctggt cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg
     7621 aaagcggtcg aacaggtggc ttatcacact ggtaattttt tattgattgg caacggttac
     7681 cacaacgaac aaaaagagcg tcaggccatt gagcaactga tccgccatcg ctgtgctgcg
     7741 ctggttgtcc atgccaaaat gatcccggat gccgatttag cctcattaat gaaacaaatg
     7801 cccggtatgg tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg tattgctctg
     7861 gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc
     7921 attggttatc tgtgctctaa ccactctatt tctgacgccg aagatcgtct gcaagggtat
     7981 tacgatgccc ttgctgaaag tggtattccg gccaatgacc ggctggtgac atttggcgaa
     8041 ccagacgaaa gcggcggcga acaggcaatg accgagcttt tgggacgagg cagaaatttc
     8101 actgcggtag cctgttataa cgattcaatg gcggcgggcg cgatgggcgt gctcaatgat
     8161 aatggtattg atgtaccggg tgagatttcg ttaattggct ttgatgatgt gctggtgtca
     8221 cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa tcgtgacgat ggcgacgcag
     8281 gctgccgaac tggctttggc gctggcggat aatcgccctc tcccggaaat cactaatgtc
     8341 tttagtccga cgctggtacg tcgccattca gtgtcaactc cgtcgctgga ggcaagtcat
     8401 catgcaacca gcgactaacc gcagttaaag caattccagc gccagtaatt cttcaatggt
     8461 ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac agaacttctg gtaacagcgg
     8521 acggctgttg tagttggatg acattgacgc gccatatgcc cctgtatcat gcagtaccag
     8581 ataatcaccc gccttcactt ccggcaaggc gcgggtttcg acatttcccc cttcctgctg
     8641 ggtaaagaca tcgcccgatt cacataacgg cccggcgacg acggtttcca ccgttggcgc
     8701 gtgttccaga gaacgaccat cagctgccag ggcactgata tggtggtaac taccgtacat
     8761 tgccgggcgc atcagatcgt tgaacccggc atcaaccaga acaaagtggc ggctacccat
     8821 ttgtttgacg ctccgcactt gagtaattaa tacgccagcc tgcgctacca gaaagcgacc
     8881 cggttcaatt tccagtttca caggatggcc caaatggcgg gcgatttgct cacgcgcggc
     8941 attccacaga ccataataat gttcggtatc aaccgcctct tcaccctgtt gataaggaat
     9001 agaaagccca ccgcccgcag aaatagcctg taaatcctga ccgaattcga ggacttgacg
     9061 caccatagca ccacatacct gttccagatg ggcataatcg acgccagaac caatgtgcat
     9121 gtgaatgccg accagctgta gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc
     9181 ggtgtaccag ataccgtgct tgctgttttc gccaccggta ttggtttttt ggctatgccc
     9241 gtgaccaaac cccggattaa cacgcagcca tacccgatgc cctggcgaaa cctgccccag
     9301 ttggtcgagc atatcaacag aacccgcatt caccggaatt tgcaattcac tgacgcgttc
     9361 aagcgtcgcc tgatcgataa catctgccgt aaaaacaata tcatcagggt gcgtttgcgg
     9421 attgtaaccc gccgccagtg cacgctctat ttcacctaac gagacggaat ccacttttac
     9481 gccctgctca cgcattaagc gcaaaatatg aatattggaa caggctttct gtgcaaagcg
     9541 caccacatca aactgtttca gcgccgcaat ctgccgacga ataatttgcg catcgtagac
     9601 ccacaccggg cagccaaatt ctgcgggcaa acgcagcaga ttttcggcgg tgagatcggt
     9661 atcagtgctg aacagtgaat gtggcataac aaactccaga taagtgcttt tttatgatta
     9721 cgccacatca taaaaagaat aaaaaatatc gatttatgtc gagtctatgc aaaaatgata
     9781 tggattaccg gattgcgaga gagcgctaat ggccgccgtt aacttacgtc atattgaaat
     9841 ttttcatgcg gtaatgaccg ccggaagcct gactgaggcg gcacacctgc tacacacctc
     9901 acagccaacc gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt
     9961 tgagcgcata cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt
    10021 gcaacgatcc tggtacggac tggatcgcat tgtcagtgcc gcagaaagtc tgcgcgagtt
    10081 tcgccaggga gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca
    10141 gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg tgccccagga
    10201 atcaccgcta cttgaagagt ggctctcggc ccagcgtcat gatttaggac tcactgaaac
    10261 gctccatacg cctgcgggaa cagaacgtac cgaattactc tctttagatg aagtgtgtgt
    10321 gttacctccg ggccatccgc tggcggtaaa aaaggtatta acgccggatg attttcacgg
    10381 tgagaactac atcagccttt cccgtactga cagctatcgc cagttgctgg atcaattgtt
    10441 tactgagaat caggttaaac gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg
    10501 cgcaatggta cgggcggggg taggcgtttc agtggttaac ccgctcaccg cactggatta
    10561 tgcggcaagc ggtttagtgg tgcggcggtt cagcattgcg gttccattca ccgtcagcct
    10621 gatccgcccc ctgcaccgcc cgtcatcagc gctggtgcag gcgtttagtg agcatttaca
    10681 agcgggatta ccgaaactgg tcacttctct tgacgctatt ttgtcgtcag ctacgacagc
    10741 ataaaagcga cagcatcctc ggcatggatc gccgcggtat caaacacagg cagaacactg
    10801 cgctcttctg gcaccagtaa accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc
    10861 tgttctgcaa ggcgagcaat cacttgcgca taataagcgc gtgacgcttc ggtaaattgc
    10921 cccagacaca gttcttcaaa aataatctga ttaattttcg cccggtcatc cgcttcagga
    10981 ataagacagt tgatggaaaa ttgttccgtc agccgcccgc gataaaaatc ctgttccatg
    11041 gtgtaacgtg tacccagcag cgccacacga gtcattcctg ccccggtaat tgcacgtcca
    11101 gtggcatccg ctatgtgtaa gaaaggcaga gagcaacgag actcaatggc atccgccact
    11161 ttgtgcatcg tattggtgca taacacaata ccttctgcgc ccgcccgctg taagccaagc
    11221 gccgcctcag ccagaatatc cccggttttg tcccactcac cgcgacgctg gcactcttct
    11281 atttcatgaa aatcgacgct atgtagcagc acttgcgcag agtgaagccc accaagccgc
    11341 tgtttaatgc cttcatttat cagacgatag taaggaatag tggattccca gctcattcct
    11401 cccagcaaac caattgtttt cattcctctc tcctgatgag tgttaccgca acaaggctga
    11461 ataaggaacg aggcaaacgc ctcaacggcc aggtgcctaa tctctattaa cgaaaaaagg
    11521 gccggatgta cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttttcgc
    11581 ctgccatcag tttgcgctcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc
    11641 agaaagtgat gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag
    11701 cgccaatgct atcaagcagt gtcaggaagg tcgcgccgat aatcatattc gacacccagt
    11761 ttgtcgtcgt tgagcaggtg atgccaaaat cgcggcattt cagcggctga atttcagagc
    11821 acaagatcca caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca
    11881 tgccaacaga gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc
    11941 agtaacccag caccagagtg cctaacgcca tcacgctgaa accaattttc agcgccggct
    12001 tacgccctgc tttatctacc gtaaagaccg caatgaaggt agcgaacata aaggtcagcc
    12061 ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga
    12121 tacgtggcgc gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca
    12181 acataccgag aaatacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc
    12241 cctgttttaa cttcaggctt tcgcgaattt cgttgagttc ttctcgcgct ttttccgacg
    12301 tatcgcgcag catacgcaat acttcttccg cctcaatatg acgccccttt tccgccagcc
    12361 agcgcgggct atttggcagg aagaccacca gaataatcag cagaactgct ggtaaagcaa
    12421 gaacccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg
    12481 ccagcacgat gccgagagtg accatcaact ggtacatgct gatcatctta ccgcgaacgt
    12541 tttcacttgc catttcagaa agatatagag gagcggtgta agacgcgatc ccgacagcaa
    12601 tgcccaacac cacacgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta
    12661 tcgaaccgag tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga
    12721 acgacagcca accattaaac agcgcaccaa ttgctgcacc gagcatcatg ctactgacca
    12781 cccattcctg caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg
    12841 cgattacgcc gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa
    12901 acatattcat acgccgcgta tcccgcagag gacgtggcgt taaagcagat tccgtattga
    12961 tagtaaccat tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaat
    13021 tcacacaggt gtcaggtcgg aaatagcata aatatggatt aaattgctac gacatgtcgt
    13081 tatgtgatgg atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac
    13141 ctaattttaa agcag
//
LOCUS       DACADA010000126        12835 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.128, whole genome shotgun sequence.
ACCESSION   DACADA010000126 DACADA010000000
VERSION     DACADA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.128"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(144..665)
                     /locus_tag="ISB17_003557"
     CDS             complement(144..665)
                     /locus_tag="ISB17_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1981448.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLDQLRNNRGN"
     gene            770..1111
                     /locus_tag="ISB17_003558"
     CDS             770..1111
                     /locus_tag="ISB17_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1981449.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            1181..2173
                     /locus_tag="ISB17_003559"
     CDS             1181..2173
                     /locus_tag="ISB17_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1981450.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDVRRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLLDAINFNPLAESVHKLSID"
     gene            2473..4917
                     /gene="dmsA"
                     /locus_tag="ISB17_003560"
     CDS             2473..4917
                     /gene="dmsA"
                     /locus_tag="ISB17_003560"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAP1981451.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            4928..5545
                     /gene="dmsB"
                     /locus_tag="ISB17_003561"
     CDS             4928..5545
                     /gene="dmsB"
                     /locus_tag="ISB17_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP1981452.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            5547..6410
                     /locus_tag="ISB17_003562"
     CDS             5547..6410
                     /locus_tag="ISB17_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP1981453.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(6446..7072)
                     /locus_tag="ISB17_003563"
     CDS             complement(6446..7072)
                     /locus_tag="ISB17_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1981454.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            7386..8534
                     /locus_tag="ISB17_003564"
     CDS             7386..8534
                     /locus_tag="ISB17_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981455.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(8631..9371)
                     /gene="pflA"
                     /locus_tag="ISB17_003565"
     CDS             complement(8631..9371)
                     /gene="pflA"
                     /locus_tag="ISB17_003565"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAP1981456.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9563..11845)
                     /gene="pflB"
                     /locus_tag="ISB17_003566"
     CDS             complement(9563..11845)
                     /gene="pflB"
                     /locus_tag="ISB17_003566"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP1981457.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(11900..12757)
                     /gene="focA"
                     /locus_tag="ISB17_003567"
     CDS             complement(11900..12757)
                     /gene="focA"
                     /locus_tag="ISB17_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAP1981458.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         3089 a         3014 c         3340 g         3392 t
ORIGIN      
        1 ttgttactcc tgtgatgtgt cgtaaattaa ttgcaattcg tccgttatta tgtttttccg
       61 caaggtattt caggttatca attaatgctt ggtcattttt tgaaatagtt attgtgaaat
      121 ctgacgtgtt tgttttcata cgattagttg cctctgttgt ttctgagttg gtccagtttt
      181 gagcttctgc ctgtataaac tcgcttaccg ttaatggtct ggcagtcttc tttattagct
      241 ggagagaact tccttttctg gcggtcatac gtccagtaac ttttccagtt gtgccattct
      301 gggggctcgt gctctggtag ctcatcagcc aacttgtccc actctttggc gttaaaccac
      361 catactccgt ctgagttttc atcttccggg tttgtgcgcg tcttgtgtcc cggcatttga
      421 ccgcgatgaa tggctacgcg cagtgcagat tttgttagcc caatatatgc cgcgcctttt
      481 tccagagagc aaagcccggc gatcggacca tccagccgca aagtcgctcc ttttttgtag
      541 gaacgctttt tcttgttttc ttcgtgctcg tcgtcgttgg cggcatcaat ggatccgctg
      601 agtgcgtcac tttcctgaag tggttggtgg tcagataact ctttatcggc aatgaagtcc
      661 cgcatttgtt atcctcttgc gttggtgcgc ttgtcgcgcc ttgtgatggt ttgttttggc
      721 ttgtaaagat aaccaaaagg ttatctttgt gcgagaggtt aatactcaaa tggttatctt
      781 gtcaatagac tacgcaaaaa agttacgtca gatacgtaag gcggaagggt taacacaaaa
      841 gcagtttgcg gatattactg ggttgtcttt ggcaaccata aggaattacg aatcggggca
      901 aaagaacgct agagcaaaaa tcgttgaggc ggttctgcaa gtcgatcgct ttgaaaaata
      961 catgctgtgg ctaataaaag ataagacgct acctgtcgcc gggcagattg ctccggctct
     1021 ctctcttgat ggatctattc agtcggaggg cgatcaggtt tcaatcggta ttacccaaaa
     1081 atcaccccga tcaggccgca atgttggctg actctacaca ttgagcgaat tacatatcgc
     1141 aaggagtgtt tagttaattt atccgttgga ggggctaatc atgtcgatta agcaactcaa
     1201 agacggacgt tatcaggtcg atgtcagacc gcagggggcg gaaggaaagc ggattcgaaa
     1261 aatctttgcc ctgaaatcaa aggctcagga gtttgagaag tatgtgttac agaactttca
     1321 tgacaagcca tggcaggcta agccagctga tcagcggcga ttatcagagc tgcttgatgc
     1381 gtggtggatg cttgatgggc gtaatcaggc ttacggggat agctacaggg ttaggctagg
     1441 gaaggttgtt cgtgaaatgg gagatcctcg cgccagtcag atgacgcgaa aatttatgct
     1501 tgaatatcgg tcagagaaat tacaggccgg attaatgccg tccagtatta atcgtgactt
     1561 atgcgtactg tctaccatgt tcacggtgct gattgaagct gaggtctttc acaacgcgaa
     1621 ccctgtacgc ggtatacgaa aactgaaagt tcagaacaca gagatggcct ttctttctga
     1681 tgatgagatt gaacggcttc ttgagcgact ggagggtgat gtgcgtcgtg ttgccattct
     1741 gtgtctttct actggtgccc gatggagtga agcatcggaa ctacgtggag agcatatcgt
     1801 tggcaaccgg gtgacgttct ttaacaccaa aaacggaaaa tcccgttcgg ttcctgtggc
     1861 ggattcggtc gtgcctctga ttaaaacccg tcgaacgggg ttattgtatc aggttgatta
     1921 tctgaagttc cgggagattc ttcaggaggt gaaaccggat ttgccgaagg ggcaggctac
     1981 gcatgtcatg cgtcatacat ttgccacaca ctttatgatg aatggcggaa atattgttac
     2041 gttgcaacga atcctggggc acgcaacgat tcagcaaaca atgacgtacg ctcatttttc
     2101 gccggacttt ttactggacg cgatcaactt taatccgctg gctgaaagtg tccataaact
     2161 gtccatcgat tagttataat ttgtgttaat ttgttccccc tgttttggct tattcgttga
     2221 aaaataagta aatcatcagg aggggcaaga aacgttaaag cgcctgcggg cgcttttttt
     2281 gtctcccttt gataccgaac aataattact cctcacttac aagtaatact actttcgagt
     2341 gaaaatccac ctatctcttt gattttcaaa ttattcgatg tatacaagcc tatatagcga
     2401 tctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gagcaagctt
     2461 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggtgagtcgc
     2521 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
     2581 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
     2641 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
     2701 gtcgtggacg gtgaaatcaa atatgttgaa acggataata ctggcgatga caactatgac
     2761 ggcctgcacc aggtgcgcgc ctgtctgcgt ggtcgttcca tgcgtcgccg cgtttacaat
     2821 cccgaccgcc tgaaatatcc gatgaaacga gtcggggcgc gcggtgaagg caaattcgag
     2881 cgcattagct gggaagaagc ctacgacatc atcgcgacca atatgcagcg cctgatcaaa
     2941 gagtacggca acgaatccat ctatctgaac tatggcaccg gtacgctggg tggcaccatg
     3001 acccgctcct ggccgccggg aaataccctg gtcgcgcgcc tgatgaactg ctgcggcggt
     3061 tatctgaacc attacggtga ctactcttcc gcgcaaattg cggaaggttt gaactatacc
     3121 tacggcggct gggcagatgg taacagcccg tcggatatcg aaaacagtaa actggtagtg
     3181 ctgtttggta ataaccctgg cgaaacgcga atgagtggcg gtggggtgac ttactatctt
     3241 gaacaggcgc gccagaaatc taatgctcgc atgatcatca tcgatccgcg ctataccgac
     3301 accggtgccg ggcgcgaaga tgagtggatc cctattcgtc cgggaacaga tgccgcactg
     3361 gttaacggtc tggcgtatgt catgatcacc gaaaacctgg tggatcaggc attcctcgat
     3421 aaatattgcg ttggctacga cgagaaaaca ctgccagcca gtgcgccgaa aaatggtcac
     3481 tataaagctt atattctggg tgaagggcca gatggcgtgg ctaaaacgcc ggaatgggcc
     3541 tcgcaaatca ctggtgttcc ggcagacaaa atcatcaaat tggctcgtga aatcggtagt
     3601 accaaaccgg cgtttatcag ccagggatgg ggcccgcagc gtcacgctaa cggtgaaatc
     3661 gcaacccgtg ctatctcgat gctggcgatt ctgaccggta acgttggtat taacggaggc
     3721 aacagcggcg cgcgtgaagg ttcatacagc ttaccgtttg tccgtatgcc gaccctcgaa
     3781 aacccgatcc agaccagcat ttcgatgttt atgtggaccg atgccattga acgtggcccg
     3841 gaaatgacag cgctgcgtga tggtgtgcgc gggaaagata agctggatgt gccgatcaaa
     3901 atgatctgga actatgccgg taactgcctg attaaccagc attctgaaat caaccgtacc
     3961 catgaaatcc ttcaggatga taagaagtgc gagctgattg tggttatcga ctgccacatg
     4021 acctcatcgg cgaaatatgc tgacatcctg ctgcctgact gcaccgcttc agaacagatg
     4081 gactttgcac tggatgcatc ctgcgggaat atgtcttacg tgattttcaa cgatcaggtg
     4141 attaaaccgc gctttgaatg taagaccatc tatgaaatga ccagcgaact ggcaaaacgt
     4201 cttggcgttg agcaacagtt tactgagggc cgtacccagg aagagtggat gcggcatctg
     4261 tatgcccagt cgcgggaagc gattcctgaa ctgccaacgt ttgaagagtt ccgtaagcag
     4321 gggattttta aaaagcgcga cccacaaggg catcacgttg cttataaagc tttccgtgaa
     4381 gatccgcagg caaatccact gaccacgcca tcggggaaaa ttgagattta ttcgcaggcg
     4441 ctggctgaca ttgccgctac ctgggaattg ccagaaggtg atgtgatcga tccactgccg
     4501 atctatacgc cgggttttga aagttatcag gatccgctga acaaacagta tccgctgcag
     4561 cttacgggtt tccactataa atctcgcgtt cactcaactt acggcaacgt tgatgtgctg
     4621 aaagcagctt gccgtcagga aatgtggatc aacccgcttg atgcgcaaaa acgcggtatc
     4681 aacaacggcg ataaagtcag gatctttaac gatcgtggtg aggttcatat tgaggcgaaa
     4741 gtgacgccac gaatgatgcc gggtgtggtc gcactgggtg aaggtgcctg gtatgacccg
     4801 gatacaaaac gtgtcgataa gggcggttgt attaacgtac tgaccactca acgtccgtct
     4861 cctctcgcta aggggaatcc gtcacataca aaccttgttc aggttgaaaa ggtgtaagga
     4921 gtaaccgatg acaacccagt atggattttt tattgattcc agccgttgca ccggttgcaa
     4981 aacctgcgag ctggcttgta aagactacaa agatttgacg ccagaagtca gcttccgccg
     5041 catttatgaa tatgctggtg gcgactggca ggaagataac ggtgtctggc accagaacgt
     5101 gtttgcctac tatctgtcga tttcatgtaa ccactgcgaa gatccggctt gtactaaagt
     5161 ctgcccgagc ggtgcgatgc ataaacgtga agatggtttt gttgtggtcg atgaagatgt
     5221 atgcattggc tgccgttact gtcatatggc ttgcccgtat ggcgcaccgc aatataacga
     5281 aacgaaaggc catatgacca aatgcgatgg ttgttatgac cgtgttgcgg agggaaaaaa
     5341 accgatctgc gtagagtcct gcccgctacg ggcgctggat ttcggcccta tcgacgagct
     5401 gcgtaaaaaa catggcgatc tggcggccgt tgcgccgttg ccgcgagctc actttaccaa
     5461 accgaatatt gtgatcaaac ccaatgccaa cagccgccca accggggata ccactggcta
     5521 tctggcaaac ccgaaggagg tgtgagatgg gaagtggatg gcatgaatgg ccgctgatga
     5581 tcttcacggt cttcgggcaa tgtgtcgcgg gtggttttat cgttctggct ttggcgctgc
     5641 tcaaaggcga cctgcgagca gaagcccagc agcgtgttat cgcctgcatg ttgggtttat
     5701 gggtgctgat gggcattggc tttatctcct ctatgcttca tcttggttca ccgatgcgcg
     5761 cttttaactc gctcaatcgg gtaggggctt cagcactcag taacgaaatc gccagcggtt
     5821 cgatcttttt tgctgtaggc ggcatcggct ggttgctggc aatgctgaaa aagctttcac
     5881 cggcattgcg tacgctttgg ctggtggtga cgatggttct tggcgtagtc tttgtctgga
     5941 tgatggtgcg tgtgtataac agcatcgata cagtacccac ctggtacagc atctggacac
     6001 cgatgggctt cttcctgacg atgtttatgg gcggcccgtt actgggttac ctgttgctga
     6061 gtctggctgg cgtcgatggc tgggcgatgc gtctgctgcc agccatttct gttctggcac
     6121 tggtagtgag tggcgtgatg tcagtgatgc agggcgcaga gctggcgact attcatagct
     6181 ctgtgcagca ggccgcagcg ctggtgccgg actatggtgc gctgatgtcc tggcggatcg
     6241 tgcttttggc cgttgccctg tgcgtgtgga ttgcaccaca gctaaaaggt tatcagcctg
     6301 cggttccgct actttctgta tcattcattc tgcttctggc aggggaatta atcggtcgtg
     6361 gcgtattcta tggtttgcat atgaccgtgg ggatggctgt cgcaaattaa tcagtaacaa
     6421 ccggggtttc ggccccggtt cgcttttatt tctgcttcgt taacgtgtca taactggtca
     6481 tcaagttacg ataatcagga atgtggttag agaacaacgt cgccagtcct tcaatatcat
     6541 tacgccagtc gcgatgcagc tcgcacgcca cgccgaacca tgtcatcaat tgcgcaccag
     6601 cttgcgacat gcgatcccat gccgaatgcc gggtaatttc attaaaggtc cctgacgcgt
     6661 cagtcacgac aaagacgtca aacccttctt caatggctga aagcgccggg aatgccacgc
     6721 aaacttcggt taccacaccg gcaataatta actgtttttt acctgtcgct ttgacagctt
     6781 ttacaaaatc ttcgttatcc caggcgttaa tatttcccgg gcgagcaatg taaggtgcat
     6841 cggggaattg tgctttcagt tctggaacta atgggccgtt aggaccggtt tcaaaactgg
     6901 tggtaagaat agttggtaag ttgaaatact tagccaggtc acccagcgcc agcacgttat
     6961 ttttaaactt atcgggttcg atatcccgta caagggaaag taaaccagcc tggtgatcaa
     7021 caagcaaaac ggcagcatca tttttatcaa gacgaacata cggtttggtc atcctcttct
     7081 cctttcgaga gtcagtggta cgtctaaatc ggtcagacgc gatgcattgc tctgaaagca
     7141 tagacgggaa atatgagttt gctgtgacca tgaaattttt cgactgaacg aagcgtatga
     7201 aatttgtgtt agatcaataa aaacaatcag atgagattaa tcgtctcttc agcattagcg
     7261 ttatgaatga gtaatataaa tcgataatac cgccgctgcg atatgcgcgt attttcaccc
     7321 attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt
     7381 aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga
     7441 ctctggcgat cgcggtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt
     7501 ctacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctt gtcggtacat
     7561 tgctgacagg gtatgtcatt aaacgcattg gctttaaccg cagctattat ctggcctcct
     7621 tcatttttgc cgctggctgt gccggccttg gcctgatgat tggattctgg agttggttgg
     7681 cgtggcgttt tgtcgctggc atcggctgtg ccatgatttg ggtggttgtt gagagtgcgc
     7741 tgatgtgcag tgggacgtcg cgtaaccgcg ggcggttgct tgctgcgtat atgatggttt
     7801 attacgtcgg aacgttttta ggccagttac tggtcagcaa agtctcaacc gagttgatga
     7861 gtgtattgcc gtgggttaca ggtttgacgt tggcaggaat cttaccgctg ttgtttacgc
     7921 gtgtcctgaa tcagcagact gaaaaccatg attcgacgtc aattacgtca atgctaaaac
     7981 tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtg ctgggatctc
     8041 tatatggcct gatgccgctg tacctcaatc acaaaggggt gagcaatgcc agcattggtt
     8101 tctggatggc ggtactggtc agtgcgggta tcctcggaca atggccgatt ggacgtctgg
     8161 cggataagtt tggtcgactg ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca
     8221 gtattgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg
     8281 gctttacgct atatccggtg gcgatggcct gggcttgtga gaaagttgaa catcatcaac
     8341 tggtggcgat gaaccaggcc ttactgttga gctataccgt gggaagcctg cttggcccgt
     8401 catttaccgc tatgctaatg cagaatttct ccgataattt attgtttatc atgatcgcca
     8461 gcgtatcgtt tatctatttg ctgatgctgc ttcgcaacgc cggccatacg ccgaaacccg
     8521 tggctcacgt gtaaatgaat tcaagcagag tgtgaactta ctgtttcaca ctctgctttt
     8581 ctgtgcacaa aaaagcggca actcaataaa gttgccgctt tacggggaaa ttagaacatg
     8641 accttatgac cgtactgctc aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt
     8701 ggtttaacac catcgagttt gtattcttca cccattgcca cccatttgtg tttgcccagt
     8761 tcgtggtagg ggaggagctc gattttctca acgttgccca tatcacgggt aaattcacca
     8821 aggcgatgcg ctgaatcgtc atcgtcagac cagcctggga caacaacata gcggatccac
     8881 accttcacat ttttgttcgc cagatattta gcgaactcca gcgtgcggtg gttggaaact
     8941 ccaaccagat tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg
     9001 gttacctcca gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtgtcc
     9061 agacaggtat gaatgccttc ttttttgcag gcgcggaacc agtcacgaac aaactcagct
     9121 tgtaggattg cctcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg
     9181 cgataggtca ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgta
     9241 tcccaggtgt cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg
     9301 ataaagcgaa tacccgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca
     9361 ataactgaca ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag
     9421 ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa
     9481 aaggctctcg tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact
     9541 gtacgatttc agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct
     9601 gctgctgttc tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca
     9661 gctgcggata tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt
     9721 taacgttcag gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca
     9781 gaccagccag gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag
     9841 agaaggtgta ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca
     9901 gagacgctac agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg
     9961 gcgcgccagc acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag
    10021 aagtgatggt cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa
    10081 ttttcttcat gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcgttgt
    10141 taccaaactg cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcatcac
    10201 gaatcggttt aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac
    10261 cagcgatacc acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg
    10321 cttcgtagct gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt
    10381 tagccagcca gtccatgaag tgatccatgc gctccatcac ttcatcatag ttcaggacat
    10441 cgcctttgat cggctcagat ttcggaccaa cctgcatttt cagtttttcg tcaacgccac
    10501 cgttgattgc gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt
    10561 gtttaccaac gatcatcggg cttacgcagc aagcaatagc gtagtcatcg ttgttgaagt
    10621 ccggacgcat caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag
    10681 cggcgaattt cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt
    10741 ccggagacgg acccatggtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca
    10801 gggtacgacc gtcgaggccc ataccaccga tagattcggt tgcccagatc gggtcgccag
    10861 agaacagttc atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca
    10921 ggtggtcaac catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt
    10981 cgatgtacac atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag
    11041 acttaacagc agccaggtag ccgaagtaag tccactggat agcttcctga gcgttagtag
    11101 ccggaccaga gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc
    11161 ggtgctgttc agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt
    11221 ccagatcagc ctgcagagag gtgaactgtg caagtttgtc tttcatcagg tagtcgatac
    11281 cgtacagtgc aacgcgacgg tagtcaccga tgatacgacc acggccataa gcatctggca
    11341 gaccggtcag aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca
    11401 cgccctggtt gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt
    11461 cgcggttgta cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag
    11521 cacgtttcag cggagcttca gtctgcagac caacgatttt ctcaagctgc ttgttgatgt
    11581 aaccagcgtc gtgagaggtg atggtggaag caacagcggt gtcaaagtca actggcgcgt
    11641 gagtgcggtt ttccagttta acgccttcca ttactttgtc ccacagggtg gtggtcgctt
    11701 cagtagcgcc agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga
    11761 agtcacggac gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg
    11821 ctaacttttc attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta
    11881 ctgcgtactt cgacaatcat taatggtgat cgttttcacg caggtaaatg acccagtatg
    11941 tcaacccaac caacaaacca ccgccgataa tattaccgat cgtaaccgga atcaggttat
    12001 cagtgatgaa gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg
    12061 tccagaattc cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt
    12121 ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga
    12181 acgctttgtc catcaggctg cggccagaat aactcatcca tactgccaga cataccatca
    12241 ggtttgccag gataccaaga cagacggcct caataaaagt atggtgcact ttgtggtcgg
    12301 cggtttgtag gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc
    12361 acattaaaag tacaaacagc agtgcgccga ccaggttgcc aaaataaaca tttagccagt
    12421 ttttcgccaa ctgaccccag gtgatgcgcc cactcgcctt ggcaacaaca atcaacactg
    12481 tggaagtaaa gagatcggct ccgcagacaa cacaaagaat caaccccaga gagaagcaaa
    12541 tgccgccaat cagttttgcc atgccgaagg gcattgtacc tgtgccagtg gttgctgtga
    12601 tatagaagac gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct
    12661 taagcggatg tttcgttgct ttatagacac ctgcctcttc ggccactttg gccattgcag
    12721 caggaagtaa aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc
    12781 ggcgacgaga tactaacaaa gcattataga tgagaaattg atatagatca tatct
//
LOCUS       DACADA010000127        12776 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.129, whole genome shotgun sequence.
ACCESSION   DACADA010000127 DACADA010000000
VERSION     DACADA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.129"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            83..1141
                     /gene="citC"
                     /locus_tag="ISB17_003568"
     CDS             83..1141
                     /gene="citC"
                     /locus_tag="ISB17_003568"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP1981459.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1156..1452
                     /gene="citD"
                     /locus_tag="ISB17_003569"
     CDS             1156..1452
                     /gene="citD"
                     /locus_tag="ISB17_003569"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP1981460.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1449..2357
                     /gene="citE"
                     /locus_tag="ISB17_003570"
     CDS             1449..2357
                     /gene="citE"
                     /locus_tag="ISB17_003570"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP1981461.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2368..3900
                     /gene="citF"
                     /locus_tag="ISB17_003571"
     CDS             2368..3900
                     /gene="citF"
                     /locus_tag="ISB17_003571"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP1981462.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3904..4455
                     /gene="citX"
                     /locus_tag="ISB17_003572"
     CDS             3904..4455
                     /gene="citX"
                     /locus_tag="ISB17_003572"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP1981463.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4430..5308
                     /gene="citG"
                     /locus_tag="ISB17_003573"
     CDS             4430..5308
                     /gene="citG"
                     /locus_tag="ISB17_003573"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP1981464.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5359..6822
                     /gene="citT"
                     /locus_tag="ISB17_003574"
     CDS             5359..6822
                     /gene="citT"
                     /locus_tag="ISB17_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAP1981465.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6936..7742
                     /gene="rna"
                     /locus_tag="ISB17_003575"
     CDS             6936..7742
                     /gene="rna"
                     /locus_tag="ISB17_003575"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAP1981466.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7972..8382
                     /gene="rnk"
                     /locus_tag="ISB17_003576"
     CDS             7972..8382
                     /gene="rnk"
                     /locus_tag="ISB17_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAP1981467.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8738..9166
                     /gene="uspG"
                     /locus_tag="ISB17_003577"
     CDS             8738..9166
                     /gene="uspG"
                     /locus_tag="ISB17_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAP1981468.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9287..10852)
                     /gene="ahpF"
                     /locus_tag="ISB17_003578"
     CDS             complement(9287..10852)
                     /gene="ahpF"
                     /locus_tag="ISB17_003578"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP1981469.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11023..11586)
                     /gene="ahpC"
                     /locus_tag="ISB17_003579"
     CDS             complement(11023..11586)
                     /gene="ahpC"
                     /locus_tag="ISB17_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAP1981470.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            11958..12704
                     /gene="dsbG"
                     /locus_tag="ISB17_003580"
     CDS             11958..12704
                     /gene="dsbG"
                     /locus_tag="ISB17_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAP1981471.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
BASE COUNT         3022 a         3370 c         3245 g         3139 t
ORIGIN      
        1 aataaaaggt tatcaatttg caacattgaa caacattcgt tgcaaatcga taacaacatg
       61 caccttcagg atactattta ttatgttcgg caatgatatt ttcacccgcg taaaacgttc
      121 agaaaataaa aaaatggcgg aaatcgccca attcctgcat gaaaatgatt tgagcgttga
      181 caccacagtc gaagtattta ttaccgtaac ccgcgatgaa aagcttatcg cgtgcggtgg
      241 aattgccgga aatattatta aatgcgttgc tatcagtgaa tccgttcgcg gtgaaggact
      301 ggcgctgaca ttagccactg aactgataaa cctcgcctat gagcggcaca gcacgcatct
      361 gtttatttat accaaaaccg aatacgaggc gctgttccgc cagtgcggtt tttccacgct
      421 gaccagcgta cccggcgtga tggtgctgat ggaaaacagc gccacgcgac tgaaacgcta
      481 tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaaa attggctgca ttgtgatgaa
      541 cgccaatccc tttacgaatg gtcaccgtta tctgattcaa caggctgcgg cacaatgcga
      601 ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc ttcccctatg aagaccggct
      661 ggatctggtg ttaaaaggca ccgccgatat tccacgcctg actgtgcatc gcggctccga
      721 atacatcatc tcccgcgcaa cgttcccttg ctacttcatt aaagaacaga gcgtcattaa
      781 ccattgctac accgaaattg atctgaagat tttccgtcag tacctcgctc ccgcgctggg
      841 tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc gttaccgccc agtacaacca
      901 ggatatgcgc tactggctgg aaacgccgac tatctccgca ccgcccatcg aactagttga
      961 aattgagcgg ctgcgttacc aggagatgcc gatatccgct tcccgggtac gtcaactgct
     1021 ggcaaaaaac gatctcacgg ctatcgcgcc gctggtccct gcagtcacgc tgcattattt
     1081 gcagaacctg cttgagcact cccgccagga cgcggcagct cgtcaaaaga cccccgcatg
     1141 agaaacaggt gaaaaatgaa aataaaccag cccgccgttg caggcaccct tgagtctggg
     1201 gatgtgatga tacgcatcgc cccactcgat acgcaggata tcgacctgca aatcaacagc
     1261 agcgttgaga aacagtttgg cgatgcaatt cgcaccacca ttctggacgt tctcgcccgc
     1321 tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag gcgcactgga ctgcatttta
     1381 cgtgcacgac tggaagccct gctggcacgc gccagcggta tcccggctct gccatgggag
     1441 gattgccaat gatttccgct tcgctgcaac aacgtaaaac tcgcacccgc cgcagtatgt
     1501 tgtttgtgcc tggtgctaat gccgcgatgg tcagcaactc cttcatctac ccggctgatg
     1561 ccctgatgtt tgacctcgaa gactccgtag cattgcgtga aaaagacacc gcccgccgca
     1621 tggtttacca cgcgctgcaa catccgcttt atcgcgatat tgaaaccatt gtgcgtgtaa
     1681 acgcgctgga ttccgaatgg ggtgttaacg acctggaagc cgtcgttcgc ggtggtgcgg
     1741 acgttgtgcg tctgccgaaa accgataccg ctcaggatgt tctggatatc gaaaaagaga
     1801 tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag taccggcctg ctggcggcga
     1861 ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc tcacgcttcc gagcgtttga
     1921 tcggtatcgc cctcggcgca gaagactatg tgcgcaacct gcgtacagaa cgctccccgg
     1981 aaggaactga actgctgttc gctcgctgct ccattttgca ggccgcgcgc tctgcgggta
     2041 ttcaggcgtt cgataccgtc tattccgacg ctaacaacga agccggattc ctgcaagaag
     2101 ccgcccacat caaacagctg ggctttgacg gcaaatcgct gatcaacccg cgtcagattg
     2161 atctgctgca caacctctac gcaccgaccc agaaagaagt ggatcacgcc cgccgcgtcg
     2221 tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt ggtttccctg aacggcaaga
     2281 tggtggacgg tccggttatc gatcgcgccc gtctggtgct ctcccgtgca gaactttccg
     2341 gcatccgcga agaataaggc aatcaaaatg acgcagaaaa ttgaacaatc tcaacgacaa
     2401 gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc ttgccgcttt ccagaactcg
     2461 ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca aactgtgcgc cgatctggaa
     2521 gaagcgattc gtcgctctgg tttacaggac ggcatgacgg tttccttcca tcacgctttc
     2581 cgtggcggtg acctgaccgt caatatggtg atggacgtca tcgcgaagat gggctttaaa
     2641 aacctgactc tggcctccag ctccctgagt gattgccatg cgccgctggt agaacacatt
     2701 cgccagggcg tggttacccg catttatacc tctggcctgc gcggtccact ggcggaagaa
     2761 atctcccgtg gtctgctggc ggaaccggtg caaatccact ctcacggcgg acgtgtgcat
     2821 ctggtacaga gcggcgaact gaatatcgat gtggctttcc tcggcgtccc gtcctgtgat
     2881 gaattcggta atgccaacgg ctactccggt aaagcctgct gcggctccct cggctatgca
     2941 atggttgatg ccgacaacgc aaaacaggtc gtgatgctta ccgaagaact gctgccttat
     3001 ccgcataatc cggcaagcat tgagcaagat caggttgatc tgatcgtcaa agttgatcgc
     3061 gttggcgatg ctgcaaaaat cggcgctggt gctacccgta tgaccactaa cccgcgcgaa
     3121 ctgcttattg cccgtagcgc tgcggatgtg attgttaact ctggctactt caaagaaggt
     3181 ttctccatgc agaccggcac cggcggcgct tcgctggcgg taacccgttt cctggaagac
     3241 aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg gcggtattac cgcgacgatg
     3301 gttgacctgc acgaaaaagg tctgatccgc aaactgctgg atgtgcagag ctttgacagc
     3361 caagctgcgc aatcgctggc ccgtaacccc aatcacatcg aaatcagcgc caaccaatac
     3421 gctaactggg ggtcgaaagg cgcatcggtt gatcgtctcg acgtggtggt actgagtgcg
     3481 ctggaaattg acacccagtt caacgttaac gtgctgaccg gctctgacgg cgtactgcgt
     3541 ggtgcttccg gtggtcactg cgataccgcg attgcctctg cgctttccat catcgtcgcg
     3601 ccgctggtac gcggtcgtat tccgacgctg gtggataacg tgctgacctg catcaccccg
     3661 ggctccagtg tcgatattct ggtcacagac cacggcatcg ctgttaaccc ggcacgtccg
     3721 gagctggcag aacgtctgca ggaagcgggg atcaaagtgg tttctattga gtggctgcgt
     3781 gaacgtgcgc gtctgctgac cggtgaacca cagccgattg aattcaccga ccgcgtcgtt
     3841 gccgttgtgc gttaccgcga tggctcggtg atcgatgttg tgcatcaggt gaaggaataa
     3901 gccatgcacc tgcttcctga actcgccagc caccatgcgg tgtcaattcc cgagctgctc
     3961 gtcagccggg atgaaaggca agcacggcaa cacgcctggc tcaagcgcca tcctgttcca
     4021 ctggtctcct ttaccgtggt tgcgccaggg ccgattaaag acagcgaggt cacacgccga
     4081 atttttaatc atggcgtgac agccttgcgt gccttagccg caaaacaggg ctggcaaatt
     4141 caggagcagg ctgcactggt ttccgccagc gggccggagg gcatgttgag cattgccgcc
     4201 ccggctcgcg acctcaagct cgccaccatt gagcttgaac atagtcatcc tctcgggcgg
     4261 ttatgggata tcgatgtcct gacgcccgaa ggcgacattc tctcccgccg cgattattca
     4321 ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag ctgtttgcgc gcgtggaaaa
     4381 acccatcaac tgaccgattt actcaaccgc atggaggcgc tgctaaacga tgtcgatgcc
     4441 tgcaacgtca actaaaacca caaagcttgc gacgtcatta attgatgagt acgccctgct
     4501 gggctggcgc gccatgctga ctgaagtcaa cctgtcaccg aaaccaggcc tcgtggatcg
     4561 cattaactgc ggtgcgcaca aagatatggc gctggaagat ttccaccgca gcgcgctggc
     4621 gattcagggc tggttacccc gtttcattga atttggtgcc tgtagtgcag aaatggcacc
     4681 ggaagcggta ctcaacggat tacgcccaat tggtatggct tgcgaaggtg atatgttccg
     4741 cgccactgcg ggcgtaaaca cgcataaagg aagcattttt tctttagggc tactgtgtgc
     4801 ggcaattggc cgtttgcttc agctcaacca gccggtaacg ccaacaaccg tttgttctac
     4861 ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg cgtaccaata attcacgact
     4921 gacggcaggt caacggttgt accagcagct tggccttacc ggcgcacgcg gtgaagccga
     4981 agcaggttat ccactggtaa tcaaccacgc cttgccgcat tacctcactc tgctggatca
     5041 ggggttagat cctgaactgg cactgctcga taccttgctc ctactgatgg ctaccaacgg
     5101 cgataccaac gttgcatcgc gcggtggcga ggggggcctg cgctggctac agcgcgaggc
     5161 gcaaacacta ttaaacaatg ggggcatccg aacccccgcc gatctcgatt atctccggca
     5221 gttcgacagg gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg acctgctgat
     5281 tcttacctgg tttttagcac agatttaatt atttaagcac ttgataaatt tggaaatatt
     5341 aattttcgga gaacccgtat gtctttagca aaagataata tatggaaact attggcgccc
     5401 ctggtggtga tgggtgtcat gtttcttatc cctgtacctg acggtatgcc gccgcaggcg
     5461 tggcattact ttgcagtgtt tgtggcaatg attgtcggca tgatcctcga gccaattccg
     5521 gcaacggcga tcagttttat tgcggttact atttgcgtta ttggcagtaa ttacctgctc
     5581 tttgatgcca aagaattagc tgacccagcg tttaatgcgc aaaaacaggc gctgaaatgg
     5641 ggtctggctg gtttttccag caccacggta tggctggtat ttggcgcatt tatttttgca
     5701 ttagggtatg aagtttccgg gttaggccgt cgtattgccc ttttcctggt gaaattcatg
     5761 gggaaacgca cgctgacgtt gggttatgcg attgtcatta tcgacattct gctggcaccg
     5821 tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt ttccggtcat taaaaacctg
     5881 ccgccgctgt ttaaatcatt cccgaacgat ccgtccgcgc gtcgtattgg cggctatttg
     5941 atgtggatga tggtcattag taccagtctg agttcgtcca tgtttgtcac cggtgcggca
     6001 ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg gtatccagat tagctggttg
     6061 cagtggttcc tctgcttcct gccggttggg gttatcttgc ttatcattgc gccgtggctt
     6121 tcctacgtgc tgtacaaacc ggaaatcaca cacagtgaag aagtggcaac ctgggcgggt
     6181 gatgaactaa aaaccatggg tgcgctgacg cgcagagagt ggacgctgat tggccttgta
     6241 ttgctcagct taggtttgtg ggtatttggc agtgaagtca ttaatgctac tgcggtaggt
     6301 ctgctggcag tttcgctgat gctggcgctg cacgttgtgc cgtggaaaga cattactcgc
     6361 tataacagcg catggaacac gctggtcaac ctggcaacgc tggttgttat ggcgaatggc
     6421 ctgacccgtt ctggctttat cgactggttc gccaacacga tgagtacaca cctggaaggc
     6481 ttctcaccaa acgcaacggt gattgtactg gttctggtgt tctactttgc acactacctg
     6541 tttgccagcc tgtctgcgca caccgcaacc atgctgccgg ttattctggc cgtcggtaaa
     6601 gggattccgg gcgtaccaat ggaacaactg tgtatcctgc tggtgctgtc tatcggtatc
     6661 atgggctgtc tgacgcctta tgcaaccggt cctggggtga ttatttacgg ctgtggctat
     6721 gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg gggtgattta catctctatg
     6781 ctgctgttgg ttggctggcc gattctcgcc atgtggaact aatacgttcg ccaaacgctg
     6841 aataaatcat cacgcccgcc aggtaagccc acctggcggg cttttttatg atttaataga
     6901 taatattcag tgtatgtatg agttccacac ccattatgaa agcattctgg cgtaacgccg
     6961 cgttgctcgc ggtttctctg cttcccttct cttctgccaa cgccgtggcg ttgcaggcaa
     7021 aacagtatgg cgattttgat cgctatgtcc tggccctctc ctggcaaacc ggattttgcc
     7081 agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg tcgcctgcaa accgaaacga
     7141 ccaacaaagc tgattttttg accgtacatg gtctgtggcc aggattgcct aaatcggttg
     7201 ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg ttgcgctact cgcccaatcc
     7261 cgaatctgcc agaagcacgc gccagccgga tgtgttcatc gccggaaacc gggttatcgc
     7321 tggaaacggc agctaaacta agtgaagtca tgccaggagc tggcggacgt tcctgcctgg
     7381 aacgctacga atatgccaaa cacggtgcct gctttggttt tgatccagac gcatacttcg
     7441 gcacgatggt acgcctgaat caagaaatta aagagagcga agccggaaaa ttccttgcgg
     7501 ataactacgg taaaacagtg agccgccgtg actttgatgc cgcctttgcc aaaagctggg
     7561 gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg taaccctgcg tatttgactg
     7621 aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc gctttctgcc aactcattct
     7681 tgccacaacc acacccaggc aactgtggca aaacctttgt gattgataaa gcaggttatt
     7741 aatcctctga tttacaaaac acgctaattc gtcttccata aaccatgacg attaagattt
     7801 atacataatg cgctacattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     7861 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     7921 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     7981 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8041 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8101 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8161 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8221 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8281 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8341 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8401 taactcgctt tgcccagagg atgcactcgc atcctctttc cgctcaaata tcaaccagat
     8461 ttatcatttt gctcttaagc gcagcattct aatctgaact ggttattcat ctgcggaacc
     8521 tcatatgagc gaagctctcg gtatcactgc attttatata ttcatagcag caattattgt
     8581 tgctgcagtg ctataccttg aacaacgctg gtagcgcctg gttttattta ttttccagct
     8641 caatagcaaa ttcagatcaa atcagcctcg caaattatag attcataacc aataatgtgc
     8701 tgttatgtat acatgcaaaa acaaggagag aatgcttatg tacaaaacaa tcatcatgcc
     8761 agtggatgtt ttcgaaatgg aattgagcga caaagcgatt cgccacgctg aattcctcgc
     8821 ccaggatgac ggagttattc atctacttca cgtactaccg gggtcagcca gcctgagcct
     8881 gcaccgtttt gccgctgatg tacgtcgttt tgaagagcat ctgcaacatg aagcagaaga
     8941 acgtttgcaa acgatggtca gccacttcac catcgatcct tcccgcatta agcaacatgt
     9001 ccgttttggt agtgtgcggg atgaagttaa cgagttagcg aaagaacttg atgcagatgt
     9061 agtagtgatt ggttcgcgta acccatcaat ttcaactcat ctgttgggtt ctaacgcctc
     9121 gagcgtaatc cgccacgcca atttgccggt gctggttgtg cgttaattgc cagaataagt
     9181 ctcccgccct gcccggagcc atccctgcag ggctttttaa tgccataaaa aagccgccag
     9241 gtttgacccc ggcggctaag caattacagg tgaatcttac ttcttcttat gcagttttgg
     9301 tgcgaatcag gtagtcaaaa gcactcagag aggctttggc accttcgcca gtggcgatga
     9361 tgatctgctt gtacggaacc gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg
     9421 tttcgcattt cgcatcaatg ataatctcgc ccatgcgatt acgttcgact gcgccttcca
     9481 gccagttggt gttcggcagc agaccaatct ggacgaaaat accggccagt tcgatgttgt
     9541 gaatatcgcc gctgacacga tcgcgatatt ccagacctac gactttgcta ccgtcgcctt
     9601 tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt tttcaggctg cgtagtttgt
     9661 cctgcagaac ctggtcggct ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa
     9721 cgatacccgc caggtcaatt gccgcttcca cgccggagtt accgccgccg ataaccgcta
     9781 cgcgtttgcc tttaaacagc gggccgtcgc agtgcgggca gtaggtcacg cctttggtgc
     9841 gatactgatc ttcgccagga acgttcatgt tgcgccattt tgcaccagtc gccacgataa
     9901 tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat ctgatgcagg ccgccttcaa
     9961 ccgctgccgg gatcagtttg ctcgcgctct ggctgtcgat cacatcaacg tcgtattcat
    10021 caacatgaac tttcagcgca cctgccagtt tctggccttc ggtcttcggt acagaaatgt
    10081 agttttcgat atcaacggta tcgaggatct gaccaccaaa acgttcgccc atcagaccgg
    10141 tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc acccgcaggg ccggaaccaa
    10201 cgattaatac gtcataagca tcacgcttgt tcagctcttc tgccgcacgt ttttccgcac
    10261 cagtatcaat tttggcaacg atttcagtca acgtcatgcg gccctgacca aactctttcc
    10321 cgtttacgaa cactgccgga acgcccatca cgttgcgatc ggtgatttcg ttctggaagg
    10381 tgccgccgtc aattgcagtg tgcttgatgc gcgggttcag tacgctcatc aggttcagcg
    10441 cctgcaccac gtccgggcag ttgtggcaag agagcgaata ataggtttcg aattcaaaat
    10501 caccgtcaat atggcgaatc tgctccagca gagactgcgc ttctttcgac ggatgaccac
    10561 cggtccacaa caacgccagt accagcgagg tgaactcgtg gcccagcggg gaacctgcaa
    10621 aacgtggccc ctggttggaa ccagggttgg taatcaggaa agacggctta cgcaccggca
    10681 agctgttatc ttctttaaag gtgactttgt ctgacagttc tgcgatttca gccaacagtt
    10741 ccttgatttc tgccgattta gcgctgtcat ccagcgtggc aattaactca acaggcttgg
    10801 tcaatttctc aaggtaagcc ttgagttgag ttttcatatt tgtgtcgagc atgtttatct
    10861 cctgggcttt aaacatcatc atgcaagcgg cctctgcggc tagctggatg cagcttgcat
    10921 catggtgcgg ggaagaaaat cgggtgccgc agcacccgaa gaattaggtg aatttccgaa
    10981 gtatctcgcg ccggagttga tagcgcgaaa gacgaaggaa atttagattt taccaaccag
    11041 gtccagagac ggagccagag ttgcttcacc ttctttccat ttagccgggc aaacttcacc
    11101 tgggtgagaa gctacgtact gtgctgcttt gattttacgc agcaggtcag acgcgtcacg
    11161 gccaatgcct tcagcggtaa cttcgattgc ctggatgata ccctgcgggt caacaacgaa
    11221 ggtcgcacgg tcagccagac cttcatcttc acgcatgttg tcgaagttac gggtcagggc
    11281 gccagtcggg tcgccgatca tcgcatattt gattttagcg atggtttcag agctgctgtg
    11341 ccatgctttg tgggtgaagt gagtatcggt agatactgcg tatacgtcta cgcccagttt
    11401 ctgcagttct tcgtaatggt cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa
    11461 gtcagccggg tagaagaaga agacgctcca gcggccttcg gtatcttttt cggtgatttc
    11521 gatgaattcg ccgtttttga atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa
    11581 ggacatctat acttcctccg tgttttcgat gagatgtaag gtaaccaatt tttgccgatt
    11641 cggctaatgc gtttccatta tcaaatcgat aagttttacc ttacaacctt cgtaagacaa
    11701 ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa atggccccct ttccaggtga
    11761 ccgtgtaatc tgaactaccc tttggtatct tcagcacctg cctgcaggct gaggtgttgc
    11821 ccttacaaat gcaacaacga catggattac aacaccctca taaacaaagg gcaatcacct
    11881 gatctaagct cttacctatg acagtgatag gttatgcctt ttactcgact tttgcactga
    11941 ctgaaaagga caaattaatg ttaaaaaaga tacttttact ggccctgctt cctgcaatcg
    12001 ccttcgcaga ggaacttcct gccccagtaa aagcgattga aaaacagggt attacaatca
    12061 tcaaaacgtt cgatgcccct ggaggaatga aaggttatct cggtaagtat caggatatgg
    12121 gcgtcaccat ctacctgact ccagatggta agcacgctat ctctggttac atgtacaacg
    12181 aaaaaggtga aaacctgagt aacacgctta tcgaaaaaga aatttacgca cccgccggac
    12241 gtgaaatgtg gcaacggatg gaacaatccc actggctcct cgacggtaaa aaagacgcgc
    12301 cggtcattgt ctacgtcttc gccgatccgt tctgcccata ttgtaaacag ttctggcagc
    12361 aggctcgtcc gtgggtagat tctggcaaag tgcaattgag aacattgttg gttggggtta
    12421 tcaagccaga aagcccggcg acagcagcgg caattcttgc ctccaaagat cccgcaaaaa
    12481 cctggcaaca atatgaagcc tctggtggca agcttaagct aagcgtgcct gcaaacgtaa
    12541 gcacagagca aatgaaagtg ttaagcgaca acgagaaact catggacgat ctgggggcaa
    12601 atgtcacacc agctatctat tacatgagta aggaaaatac cctgcaacaa gccgtcggat
    12661 tacccgatca gaaaacgctt aatatcatta tgaggaataa ataaaagata attcatggat
    12721 ggttgaaata catcattgct tttcaaccat cctaaaaacg catgaattgc tcgtaa
//
LOCUS       DACADA010000128        12535 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.130, whole genome shotgun sequence.
ACCESSION   DACADA010000128 DACADA010000000
VERSION     DACADA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.130"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1513
                     /gene="caiT"
                     /locus_tag="ISB17_003581"
     CDS             <1..1513
                     /gene="caiT"
                     /locus_tag="ISB17_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAP1981472.1"
                     /translation="KNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFS
                     YVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAA
                     VLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFF
                     FVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECM
                     QWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVS
                     GASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFL
                     ARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVARA
                     IIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIG
                     WSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKY"
     gene            1544..2686
                     /gene="caiA"
                     /locus_tag="ISB17_003582"
     CDS             1544..2686
                     /gene="caiA"
                     /locus_tag="ISB17_003582"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAP1981473.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2815..4032
                     /gene="caiB"
                     /locus_tag="ISB17_003583"
     CDS             2815..4032
                     /gene="caiB"
                     /locus_tag="ISB17_003583"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAP1981474.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4105..5658
                     /gene="caiC"
                     /locus_tag="ISB17_003584"
     CDS             4105..5658
                     /gene="caiC"
                     /locus_tag="ISB17_003584"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAP1981475.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDIYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSVRAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSVNDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5767..6552
                     /gene="caiD"
                     /locus_tag="ISB17_003585"
     CDS             5767..6552
                     /gene="caiD"
                     /locus_tag="ISB17_003585"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAP1981476.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6558..7148
                     /gene="caiE"
                     /locus_tag="ISB17_003586"
     CDS             6558..7148
                     /gene="caiE"
                     /locus_tag="ISB17_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAP1981477.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVLAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7267..7662)
                     /gene="caiF"
                     /locus_tag="ISB17_003587"
     CDS             complement(7267..7662)
                     /gene="caiF"
                     /locus_tag="ISB17_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAP1981478.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7923..11144)
                     /gene="carB"
                     /locus_tag="ISB17_003588"
     CDS             complement(7923..11144)
                     /gene="carB"
                     /locus_tag="ISB17_003588"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP1981479.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11162..12310)
                     /locus_tag="ISB17_003589"
     CDS             complement(11162..12310)
                     /locus_tag="ISB17_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAP1981480.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         2922 a         3277 c         3324 g         3012 t
ORIGIN      
        1 gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc cgttaataat
       61 cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg tcgttattaa
      121 tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt
      181 gatgcttttc ggctggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa
      241 cgaaccacca gaatttagta ccgccagttg gatctttatg atgttcgcct cctgtacgtc
      301 tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt
      361 tggcttagaa ccgaactcaa caggcgcgaa agagttgggg ctggcttaca gcttgttcca
      421 ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt
      481 ctttgtccgc aaaatggaag tgattcgccc cagctcgacc ctggtgccgc tggtaggtga
      541 aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg tcgccttgat
      601 cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtaaccgagt gtatgcaatg
      661 gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct gctggattat
      721 cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt
      781 gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg gtgccagctt
      841 catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt
      901 gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg
      961 ggcatggtgg gtgatttacg ctatccagat gagtatcttc ctcgcccgca tctcccgtgg
     1021 tcgtaccgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt caacctggat
     1081 cctgtggact gtactcggta gtaacaccct gctgttgata gataaaaaca tcatcaacat
     1141 tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct gggccgctct
     1201 gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg ccaccgttac
     1261 gctggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag tacgtgacgg
     1321 cgaagaaccc ccgctgctgg tgcgcattgg ttggtcagtt ctggttggca ttatcggcat
     1381 tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg ccggaggatg
     1441 cccgctgttc ttcgtcaaca tcatggtgac gctctccttt attaaagacg cgaaacagaa
     1501 ctggaaatat taattaaccc caaaaatatc aagaggttga aagatggatt ttaatttaaa
     1561 tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga
     1621 ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagccct
     1681 ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc tggacgcggg
     1741 gtttgttact ctcgccgccg tgtggatgga actgggtcgt ctgggagcgc caacttacgt
     1801 gctgtatcag ttaccgggcg ggtttaacac cttcctgcgt gaaggtacgc aagagcagat
     1861 cgacaagatc atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga
     1921 accgggcgca ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa
     1981 gatttatctt aatggtagta agtgttttat taccagcagc gcctacaccc cgtacatcgt
     2041 ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga
     2101 tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc gtatggatag
     2161 ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt tcggtcggga
     2221 aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct
     2281 gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg ccaaccagcg
     2341 cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat
     2401 ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa
     2461 cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt
     2521 tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gtaatcaccg
     2581 catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat
     2641 gcagatcctg acgctgggcc gcgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc
     2701 tgatgcgacg ctgacgcgtc ttatcaggcc tacaggagca cgaatgtcgg ccggataagg
     2761 cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct aataatggat
     2821 catctaacca tgccgaaatt cgggccgctg tccggattgc gcgttgtctt ctccggtatc
     2881 gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga agttatctgg
     2941 atcgaaaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcaactttcc
     3001 cgccgcaacc tgcacgcgct gtcgttaaat atcttcaaag atgaaggtcg tgaagcgttt
     3061 ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc
     3121 cgtcgtggca ttaccgatga agtcctgtgg cagcacaacc caaaactggt tatcgctcac
     3181 ctgtccggtt ttggtcagta tggcaccgag gagtacacca atcttccggc ctataacact
     3241 atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct
     3301 gccttcccgt ataccgccga ttacttttct ggcctgactg ccaccacggc ggcgctggca
     3361 gcactgcata aagtgcgtga aaccggcaaa ggcgaaagca tcgacatcgc catgtatgaa
     3421 gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc
     3481 ccgcgcatga ccaaagggaa agatccctac tacgccggtt gcggtctgta taaatgcgcc
     3541 gacggctaca tcgtgatgga actggtgggc attactcaaa ttgcagagtg ctttaaagat
     3601 attggcctcg cacatctgct cggcacgcca gaaattccgg aaggcaccca gcttatccac
     3661 cgtatcgaat gcccttacgg cccactggta gaagagaaac tcgatgcctg gctggcggca
     3721 cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta
     3781 ctgaccgtac cggaactgga aagcaatcca cagtatgtgg ctcgcgaatc aatcactcag
     3841 tggcaaacga tggatggtcg cacctgcaaa gggccgaata tcatgccgaa attcaaaaat
     3901 aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg
     3961 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa
     4021 gttgaggact aaacgcgtgg cgtcagtcag cttgcctgac tagtgcccgc tcaataacaa
     4081 acgaacaaaa tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg
     4141 tgggacgatc tggcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcagc
     4201 ggagtcgtta accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac
     4261 ctgttttata cgctggggat tcgcaaaggc gacaaggttg ctctacatct cgacaactgc
     4321 ccggaattta tcttttgctg gttcgggttg gcaaaaattg gcgcgattat ggtgccgatt
     4381 aacgcccgcc tgttgcgcga agaaagcacg tggatcctgc aaaatagcca ggcgtgcctg
     4441 ctggtgacca gtgcgcaatt ttatcctatg tatcaacaga ttcagcagga agatgcctct
     4501 cagttgcggc acatttgcct gacagatatg gcacttcccg ctgatgatgg cgtgagttcg
     4561 tttactcaac tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact
     4621 gacgatacgg cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggggtggtg
     4681 ataactcatt acaacctgcg cttcgctgga tattactccg cctggcagtg cgcactgcgt
     4741 gatgatgaca tctacctgac ggtaatgcct gcatttcata tcgattgcca gtgtactgcg
     4801 gcaatggcgg cattttctgc cggggccacc tttgtgctgg tcgagaaata cagcgtccgc
     4861 gccttctggg ggcaggtaca gaagtaccgt gccaccgtta ccgaatgtat tccgatgatg
     4921 atccgtaccc tgatggtaca accaccttct gtgaatgaca ggcaacactg cctgcgggaa
     4981 gtgatgtttt atctcaacct gtcagagcag gaaaaagacg ctttttgtga acgcttcggc
     5041 gtccgcttgc tgacgtctta cgggatgacg gaaaccattg ttggcattat cggcgatcgc
     5101 cctggcgata aacgacgctg gccgtcaatt ggtcgggcgg ggttttgcta cgaagcggag
     5161 atccgcgacg atcacaatcg cccgctcccg gcaggtgaaa tcggtgaaat ctgtattaaa
     5221 ggcgtaccag ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcgaaa
     5281 gtgctggaag ccgatggctg gctacatacc ggcgataccg gataccgcga cgaagagggc
     5341 tttttttatt tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc
     5401 tgcgtggagc tcgaaaatat catcgccacc catcccaaaa ttcaggatat cgtggttgtg
     5461 ggtattaaag attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt
     5521 gaaacattga gcgaagagga atttttccgc ttctgcgaac aaaatatggc gaaatttaaa
     5581 gtgccctctt atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt
     5641 agaaagaatc tgaaataaat gaaacggcag ggaactaccc tgtcagctaa taatcacgct
     5701 ctaaaacaat atgcattttt tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga
     5761 aaaggaatga gtgaatcatt acatctgacc cgcaatggat caattctgga aattaccctt
     5821 gatcgtccaa aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt
     5881 ctaaatttcc gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaaa
     5941 ttcttttccg cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac
     6001 tttggcccgg gaggttttgc gggattaacc gaaattttca atctcgacaa accggtcatc
     6061 gccgctgtga acggctatgc ctttggcggc ggctttgaac tggcgctggc ggcagatttt
     6121 attgtttgtg ccgataacgc cagcttcgcc ctaccggaag ccaaactggg catcgttcct
     6181 gacagcggcg gcgtgctgcg tctgccgaag atcctgccgc ctgccatcgt caatgaaatg
     6241 gtgatgaccg gcagacgaat gggcgcagaa gaggcgctgc gttggggggt agtcaaccgc
     6301 gtggttagcc aggcggaact gatggataac gcccgcgaac tggcgcagca gctggttaac
     6361 agcgctccgc tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg
     6421 gtagaagaag cgtatcgcta tattcgcagc ggcgtgttga aacactatcc atcggttctg
     6481 cattcggaag atgccattga agggccgctg gcgtttgccg agaagcgcga tccagtgtgg
     6541 aaaggacgtt aacaaccgtg agctattacg cctttgaggg gttaattccg gttgttcacc
     6601 cgacggcgtt tgtccatccc agtgcagtat tgattggcga tgtgattgtg ggagccggtg
     6661 tctacatcgg cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgctagcgg
     6721 gagccaatat tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg
     6781 gggaaaacgg tcatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg
     6841 cactggtcgg gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg
     6901 ttgccgctat gagctttgtc aaagcgggct ttcgcggcga gaaacgccag ttgttgatgg
     6961 gtacgccagc ccgcgctgta cgcagcgtga gtgacgacga gttacactgg aaacggttga
     7021 ataccaaaga gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc
     7081 cactgaggca aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccga
     7141 aacggtgatc ttgccggatg cggcgtaaac gccttatccg acctggctgg aaagcaaccg
     7201 taggccggat aagatgcgcc agcatcgcat ccggcaacca ggctgcgtgg tatcaacgaa
     7261 atgacattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
     7321 ttccgtgagc ggaacggcag gaatacgcgg cgtctttctt acgccgacta atttatcccg
     7381 gctgttattg cgcagacgcg cgtaaatttg ctcatcgata tcaaccactt taaccagtcg
     7441 ctgacactgg catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat
     7501 ttctgtgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
     7561 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
     7621 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
     7681 aattaaaaga ctggctatga ctcgttttgc agcaattaaa caaggccata ttaaaattgt
     7741 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt
     7801 catcaaaaat atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc
     7861 agagaaccgt attagttatt tgatcaaatt agcggatgaa aaatatccgc catgacacgc
     7921 tattatttga tctgcgcgtg catttcctgc accgaaatta ctttttcggt cgcatcggca
     7981 ttcagcgcca tcgcggtggc aaagccgccg ttcagggtgg tgtcgtaatg cactttatat
     8041 tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacgacc tgaggtggtg
     8101 ttgatgatgt aggtatattc gccattctta atacggtcct gaatgtgcgg acggccttca
     8161 tgcaccttgt tcaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg
     8221 tgagtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt
     8281 tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg
     8341 ctgcccagct gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catgacttcc
     8401 ccggtagagc gcatttctgg ccctaacagc gggtcaacgc ccgggaattt attgaacggc
     8461 agcaccactt ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca
     8521 gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct
     8581 ttggagacga acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg
     8641 ttgtttttca ccgcaaactg cacgttcatc aggccgcgca cctgcagttc gaaggccagt
     8701 ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta ggctggcagt
     8761 gaacatgcgg agtcaccgga gtgcacaccc gcctgctcaa tatgctccat gatgccgcca
     8821 atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg
     8881 aagtggtcca gcaacactgg cgcatcgtta gatacgctga ccgctgtctg gaagtagcga
     8941 cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga
     9001 cgcaccacca gcgggtagcc aatctctttc gctttctcaa ccgccatttc gatggtggta
     9061 acggtggcgt tcgcaggttg tttcagtttc agacgctcaa ccgcatgctg gaagcgttca
     9121 cggtcttccg cacggtcgat ggcatccggg ctggtaccga taaccggtac gccagcagct
     9181 tccagcgcgc gcgccagttt cagcggggtc tgaccgccgt actggacgat aacgcctttc
     9241 ggcttctcaa tacgcacaat ttccagcacg tcttccagcg ttaccggctc gaagtagaga
     9301 cggtcagagg tgtcgtagtc ggtggagacg gtttccgggt tacagttaac cataatggtt
     9361 tcataaccat cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc gaattcgata
     9421 ccctgaccga tacggttcgg gccgccgccg agcaccatga ttttttcacg atcggtggac
     9481 ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac
     9541 tctgccgcac aggtatccac gcgcttataa accgggtgca agtcatactg gtcgcgcagc
     9601 ttgcggattt ctgcttcacg cacgcccgcg agttttgcca gacgcgcatc ggcaaagcct
     9661 ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttctgcgact
     9721 ttctcttcca gacgcaccag ctcttcaatc tgtaccagga accagcggtc aatgttggtc
     9781 aaattaaaca cgccgtctac agacaggccc gcgcggaagg catcagcgat ataccagata
     9841 cgctctgcgc ctgcatcttt cagttcgcgg cggattttag tcagcgcttc cgggtcgtcg
     9901 agactcactt tcggatcgaa gccagttgcg cccacttcca ggccgcgcag cgctttttgc
     9961 agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg
    10021 gtcagacggt cgttagcacc ggcgaatttc tcgaagttga agcgaggaat tttagtaacc
    10081 acatagtcga tggacggctc gaaggaggcc ggagtacgcc caccggtgat gtcgttcatc
    10141 agctcgtcga gggtgtaacc caccgccagt ttcgccgcca ctttggcaat cgggaaacca
    10201 gtggctttcg acgccagcgc agaggagcga gacacgcgtg ggttcatttc gataacaatc
    10261 aggcgaccgt ttttcgggtt caccgcaaac tggacgttgg aaccaccggt ttcaacgccg
    10321 atttcacgca gtaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc
    10381 gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt
    10441 tcgatagagc agacgatgat acagttgtcg tttttatcac gcaccacttc catctcgtac
    10501 tctttccagc cgatcagcga ctcatcaatc agcagctctt tagtcggaga aagatccaga
    10561 ccgcgggcgc aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg
    10621 cccatggtaa aggatgggcg aataatgcac gggaagccaa cttcagcggc aaccgccagc
    10681 gcttcttcca ttgaatgagc gataccggaa cgcgcagttt ccagaccgat tttcttcatc
    10741 gctacgtcga aacggcggcg gtcttcggct ttatcaatcg catcggcagt ggcaccaatc
    10801 atggtgacgc cgaactcttc cagcacgccc tgacgctcaa gctccagcgc acagttcagc
    10861 gccgtctgcc cccccatggt tggcagcacc gcgtccgggc gctctttttc aataatcttg
    10921 cgtaccactt cccagtgaat cggctcgatg taggttgcat cagccatttc cgggtcggtc
    10981 atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc acgcagggct
    11041 ttacacgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc
    11101 gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg
    11161 attacttagc ggttttacgg tactgctcaa ttaacgcgat aaagtggtca aacaacggcg
    11221 cggcgtcgtg tggaccaggg ctggcttccg ggtgtccctg gaagctgaac gccggtttat
    11281 cggtacgatg aatgccctgt aacgtaccgt cgaacaggga tttatgtgtg acacgcaggt
    11341 ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca
    11401 catttttctc aacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga
    11461 cagtcttcgc accgctcgcc agcgccagca gctgatggcc gaggcagatg ccgaataccg
    11521 gaatatcggt ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt
    11581 cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag
    11641 aagtttgcgc cggaacgatg gtcagacgac agcctctatc caccagcatc cgcaggatgt
    11701 tgcgcttggc accaaaatca taagccacga cgtggtacgg cagctcgtct tcttttttcg
    11761 cttctggcag accgccggtc agcgtccagc tcccttgtgt ccagctatag ggttctgcgg
    11821 tagtcacttc ttttgccaga tccatgccat tcaggccagg gaacgcgcgg gctttttcta
    11881 acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct
    11941 cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc
    12001 gcttcaggta agaagagagg tcttcggtat tacgaaagtt gctggcaatc agcggcaggt
    12061 cgcgaatcac cagaccttgg gcatgtacct gagaagattc ttcatcggcg tcattagtgc
    12121 cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag
    12181 tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac
    12241 ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg
    12301 acttaatcaa aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa
    12361 tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc
    12421 tggagatatg ggagctaaaa gtcaacttaa tggcgggatt ttttatatta ttttgtggct
    12481 catgcatgtt ttacaggcat taagcgacaa aaagatcata taacatgttc tggaa
//
LOCUS       DACADA010000129        12518 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.131, whole genome shotgun sequence.
ACCESSION   DACADA010000129 DACADA010000000
VERSION     DACADA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.131"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            212..1084
                     /locus_tag="ISB17_003590"
     CDS             212..1084
                     /locus_tag="ISB17_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP1981481.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1412..4540
                     /gene="ag43"
                     /locus_tag="ISB17_003591"
     CDS             1412..4540
                     /gene="ag43"
                     /locus_tag="ISB17_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP1981482.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGT
                     LAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNT
                     TLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGN
                     MLQAGAFNYTLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     NGENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKA
                     SSDNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSS
                     RGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGNSAE
                     GYNGQATLNVTF"
     gene            4602..5762
                     /locus_tag="ISB17_003592"
     CDS             4602..5762
                     /locus_tag="ISB17_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981483.1"
                     /translation="MFNATFAGINSAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVE
                     TMKNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSL
                     AAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGM
                     LKHIDFSTASPTVRQALQKGTGAGNISALNKVMKGSLVTSLALVAVTTGPDMSKMLRG
                     RISGAQFIRNLAVASSCVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAV
                     SGKIAGALVEEDRVKILAMIQEQMTWLAGSFLLTGHEIENLNANLARVIDQNALEIIF
                     AAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEETLPPELPA"
     gene            5838..6293
                     /locus_tag="ISB17_003593"
     CDS             5838..6293
                     /locus_tag="ISB17_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981484.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            6372..6605
                     /locus_tag="ISB17_003594"
     CDS             6372..6605
                     /locus_tag="ISB17_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1981485.1"
                     /translation="MLGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEADAGKQLNAWLASGGLLRQ"
     gene            6706..7524
                     /locus_tag="ISB17_003595"
     CDS             6706..7524
                     /locus_tag="ISB17_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1981486.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            7579..8064
                     /locus_tag="ISB17_003596"
     CDS             7579..8064
                     /locus_tag="ISB17_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1981487.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            8080..8556
                     /locus_tag="ISB17_003597"
     CDS             8080..8556
                     /locus_tag="ISB17_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1981488.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            8619..8840
                     /locus_tag="ISB17_003598"
     CDS             8619..8840
                     /locus_tag="ISB17_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1981489.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            8859..9503
                     /locus_tag="ISB17_003599"
     CDS             8859..9503
                     /locus_tag="ISB17_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP1981490.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            9553..9921
                     /locus_tag="ISB17_003600"
     CDS             9553..9921
                     /locus_tag="ISB17_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1981491.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCDYVYLAVYPTPEMKN"
     gene            10010..10387
                     /locus_tag="ISB17_003601"
     CDS             10010..10387
                     /locus_tag="ISB17_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP1981492.1"
                     /translation="MKTLPDTHVRAASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            10384..10872
                     /locus_tag="ISB17_003602"
     CDS             10384..10872
                     /locus_tag="ISB17_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981493.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASTECLPDLPTF
                     DW"
     gene            10889..11086
                     /locus_tag="ISB17_003603"
     CDS             10889..11086
                     /locus_tag="ISB17_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1981494.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            11171..12016
                     /locus_tag="ISB17_003604"
     CDS             11171..12016
                     /locus_tag="ISB17_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAP1981495.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            12085..12480
                     /locus_tag="ISB17_003605"
     CDS             12085..12480
                     /locus_tag="ISB17_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HAP1981496.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKSQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
BASE COUNT         3014 a         3375 c         3407 g         2722 t
ORIGIN      
        1 cctttacgga gtaatcagcc tgctgtttaa caataaccgc gtattttcat ggaaggatat
       61 cacccaataa cggataactc ctgccctcct tcatgttaat acgcaacatc cccatcgatt
      121 ccaaacaatt tcagacagat attatctccg tggatccccc gccacctttc cggtgtgcgg
      181 ggttttgtct tttttcaccg ggaatacatg tatgaatccg tctgatgcca ttgaggcaat
      241 tgaaaaaccg ctctcctccc tgccttactc gctttcccgt cacatcctgg aacatctgcg
      301 caaactcacc cgtcacgagc ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc
      361 ttcactctgt aatgccctgt ttcaggggga ggtcacaccg gtcagtgatg ttcacgccgg
      421 cacccgggaa gtgcagcgct tccgtctgag tggtcacggt tacagcatgg ttatcactga
      481 cctgcccggg gtgggcgaga gccgggacag ggatgcagag tatgaagccc tgtaccgtga
      541 cattctgcct gaactggacc tggtgctgtg gctgattaaa gccgatgacc gggccctgtc
      601 tgtggatgag tatttctggc gacacatcct gcagtgcgga catcagcagg tgctgtttgt
      661 ggtgacacag gccgacaaaa cggagccctg ccatgaatgg gatatggccg gcattcagcc
      721 ctctcccgca caggcacaga acattcgcga aaaaacggag gcggtattcc gtctgttccg
      781 gcctgtacat ccggttgtgg ccgtatcggc ccgcaccggc tgggaagtgg atacgctggt
      841 cagtgcgctc atgacagcgc ttcccgacca tgccgccagt cccctgatga cccgcctgca
      901 ggacgagctg cgcacggaat ctgtccgcgc tcaggcccgt gagcagttta ccggtgcggt
      961 ggaccggata tttgacacag cggagagcgt ctgtgttgcc tctgttgcac gcacggttct
     1021 gcgtgccgtc cgtgacacag tggtctctgt tgcccgcgcg gtatggaact ggatcttctt
     1081 ctgaacctgt tgtggatgat gtcctccctg cctctaagta tgctcacaaa agcgctgttt
     1141 tcgttactgt ctctcttgtc cgtgcaatag cttgataaaa gaataaaacg atcaataccc
     1201 attttaccga tcgttaatat cgatcgttat gctaataata actcctgtta gcaacgtgcg
     1261 cagatacaca cagacatgag attcagggaa caacagagcc acacgtcaga aacttccgtc
     1321 agccggacct ccggcactgt aaccctttac ctgccggtat ccacatctgt ggataccggc
     1381 ttttttattc accctcactc tgattaagga aatgctgatg aaacgacatc tgaatacctg
     1441 ctacaggctg gtatggaatc acattacggg cgctttcgtg gttgcctccg aactggcccg
     1501 cgcacggggt aaacgtggcg gtgtggcggt ggcactgtct cttgccgcgg tcacgtcact
     1561 cccggtgctg gctgctgaca tcgttgtgca cccgtgtgaa acagtgaatg gcggaacact
     1621 ggtaaaccat gacaaccagt ttgtatccgg aacagctgat ggcgtgactg tcagtaccgg
     1681 gcttgagctg gggccggaca gtgacgaaaa caccggcggg caatggataa aagcgggtgg
     1741 cacaggcaga aacaccactg tcaccgcaaa tggtcgtcag attgtgcagg caggaggaac
     1801 tgccagtgat acggttattc gtgatggcgg agggcagagc cttaacggac tggcggtgaa
     1861 caccacgctg gataacagag gtgagcagtg ggtacacggg ggagggaaag ctgccggtac
     1921 aattattaac caggatggtt accagaccat aaaacatggc ggactggcaa ccggaaccat
     1981 cgtcaacacc ggtgcagaag gtggtccgga gtctgaaaat gtgtccagcg gtcagatggt
     2041 cggagggacg gctgaatcca ccaccatcaa taaaaatggc cggcaggtta tctggtcttc
     2101 ggggatggca cgggacaccc tcatttacgc cggtggtgac cagacggtac acggagaggc
     2161 acataacacc cgactggagg ggggtaacca gtatgtacac aacggtggca cggcaacaga
     2221 gacgctgata aaccgtgatg gctggcaggt gattaaggaa ggaggaactg ccgcgcatac
     2281 caccatcaac cagaaaggaa agctgcaggt gaatgccggc ggtaaagcgt ctgatgtcac
     2341 ccagaacacg ggcggtgcac tggttaccag tacagctgca accgtcaccg gcacaaaccg
     2401 cctgggagca ttctccgttg tggcgggtaa ggctgataat gtcgtactgg aaaatggcgg
     2461 acgtctggat gtgctgagcg gacacacagc cactaatacc cgtgtggatg atggcggaac
     2521 gctggatatc cgcaacggtg gtgccgccac caccgtatcc atggggaatg gcggtgtact
     2581 gctggccgat tccggtgccg ctgtcagtgg tacccggagc gacggaaagg cattcagtat
     2641 cggaggcggt caggcggatg ccctgatgct ggaaaaaggc agttcattca cgctgaacgc
     2701 cggtgatacg gccacggata ccacggtaaa tggcggactg ttcaccgcca ggggcggcac
     2761 actggcgggc accaccacgc tgaataacgg cgccacactt accctttccg ggaagacggt
     2821 gaacaacgat accctgacca tccgtgaagg cgatgcactc ctgcagggag gcgctcttac
     2881 cggtaacggc agcgtggaaa aatcaggaag tggcacactc actgtcagca acaccacact
     2941 cacccagaaa gccgtcaacc tgaatgaagg cacgctgacg ctgaacgaca gtaccgtcac
     3001 cacggatgtc attgctcagc gcggtacagc cctgaagctg accggcagca cagtgctgaa
     3061 cggtgccatt gaccccacga atgtcaccct gacatccggt gccacctgga atatccccga
     3121 taacgccacg gttcagtcgg tggtggatga cctcagccat gccggacaga ttcatttcac
     3181 ctccgcccgc acagggaagt tcgttccgac aactctgcag gtgaaaaacc tgaacgggca
     3241 gaatggcacc atcagcctgc gtgtacgccc ggatatggcg cagaacaatg ctgacagact
     3301 ggtcattgac ggtggcaggg caaccggaaa aaccatcctg aatctggtga acgccggcaa
     3361 cagtggtacg gggctggcga ccaccggtaa gggtattcag gtggttgagg ccattaacgg
     3421 tgccaccacg gaggaagggg cttttgtcca ggggaatatg ttgcaggccg gggcctttaa
     3481 ctacaccctc aaccgggaca gtgatgagag ctggtatctg cgcagtgaaa atgcttatcg
     3541 tgctgaagtc cccctgtatg cctccatgct gacacaggca atggactatg accggattct
     3601 ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa aataacagcg tccgtctcag
     3661 cattcagggc ggccatctcg gtcacgataa caacggcggt attgtccgtg gtgccacgcc
     3721 ggaaagcagc ggcagctatg gcttcgtccg tctggagggt gacctgctca gaacagaggt
     3781 tgccggtatg tcactgacga caggagtgta tggtgctgca ggccattctt ccgttgatgt
     3841 taaggatgat gacggctccc gtgccggcac ggtccgggat gatgccggca gcctgggcgg
     3901 atacctgaat ctggtacaca cgtcctccgg cctgtgggct gacattgtgg cacagggaac
     3961 ccgccacagc atgaaagcgt catcggacaa taacgacttc cgcgcccggg gccggggctg
     4021 gcagggctca ctggaaaccg gtctgccatt cagtatcact gacaacctga tgctggagcc
     4081 acaactgcag tatacctggc agggactttc cctggatgac ggccaggata acgccggtta
     4141 tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc ggtttccgtc tgggcagcca
     4201 caacgatatg acctttggtg aaggcacctc atcccgtgac accctgcgcg acagtacaaa
     4261 acacggtgtg agtgaactgc ctgtgaactg gtgggtacag ccttctgtta tccgcacctt
     4321 cagctcccgg ggagacatga gcatgggtac agccgcagcc ggcagtaaca tgacgttctc
     4381 accgtcccgg aatggcacgt cactggacct gcaggccgga ctggaagccc gtgtccggga
     4441 aaatatcacc ctgggcgttc aggccggtta tgcccacagt gtcagcggca acagcgctga
     4501 aggctataac ggtcaggcca cgctgaatgt gaccttctga cagaaccatc gcctctttgt
     4561 ggtcccgatc atcatgatcg ggacccggac cggcgcaacg gatattcaac gccacattcg
     4621 ctggcattaa cagtgcggca ttcgacggac agaacggaca gtatcgttac atgggaaatc
     4681 atggtcccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga
     4741 agaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
     4801 tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca
     4861 tcgaatcggt cacttatgat attgccgagg ggtcggttgt cagtctggcg gccggaggga
     4921 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
     4981 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
     5041 caacccagca acttcaccgg ctcagtgttg ttcagggtat gctgaagcat attgattttt
     5101 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
     5161 gtgccctgaa caaagtgatg aagggttcgc tggtgacatc tctggcactg gtagctgtca
     5221 caaccggccc tgacatgagc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
     5281 ggaatcttgc cgtggcatct tcctgtgtgg caggtggtgc tgtcgggtca gtggcgggcg
     5341 ggatattgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg
     5401 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
     5461 aagatcgcgt caaaattctg gcaatgattc aggagcagat gacatggctt gccggcagtt
     5521 tcctgctgac cggacatgag attgaaaatc tgaacgcgaa tctggcccgt gttatcgatc
     5581 agaatgctct ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaacatgt
     5641 taatcaaacc actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat
     5701 cccatctcgg caatatggtt aaccgactgg aagaaacatt acccccggag ttaccggcat
     5761 aacggctatt ctgcgccttt tacgttttat cacctgattt gtcagtgtca tcgtcatgac
     5821 tcagagaggt aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc
     5881 tggggttaac gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca
     5941 atacagatac gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg
     6001 gcggaaacga tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg
     6061 gtcagacgct ggtgaccaga aaaatggaaa cggaagcctt tggtgacagt aatgccacgc
     6121 gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca
     6181 ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcgatc
     6241 cccaggacta ccatccactg ctgattaccg tttccggaaa aaacgttaac tgatccctga
     6301 agtcagtttc atgcttcagc ctctgtcaca acgcgtggca gaggcttttt atttatcagg
     6361 agaaaacaga tatgctgggt tacacagagt atgtcctggc tgaaggatct ttctcatacg
     6421 ggcaggcggt tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa
     6481 tgcatttccg tcgggttatc cgcaacgcgg aaggtcagcg acgctggcgc tgccggaact
     6541 ctgaagctga tgccggaaaa cagcttaatg cgtggctcgc cagtggaggg cttctcagac
     6601 aataaacctc attgatttca tccatcaggc cgcgtcttct ccgggagacg cggctttttt
     6661 catttattac accagtaaat cttaaccacc gataaggagc aaattatgcg actagccagt
     6721 cgttttggtt atgcgaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
     6781 cactatgtgc cggggatttt cggggaagat aaacacacgt cccgaagcca gaactatacg
     6841 tacatcccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     6901 gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca catgctgcgt
     6961 ctgcggcggg ccggagagat aaacggagaa catgtccctg aaattattct gctcaactct
     7021 catgacggta cctccagcta ccagatgctg ccgggttact tcaggttcgt ctgccagaac
     7081 gggtgcgtct gtggtcagtc tctgggggaa gtgcgtgttc cgcaccgggg aaatgtggtg
     7141 gacagagtca ttgaaggggc ttacgaggta gtgggcgtgt ttgaccggat tgaggaaaag
     7201 cgtgatgcca tgcagtcgct gattctgccg ccaccggcac gccaggcgct ggcacaggcg
     7261 gcactgactt accgttatgg tgacgaacat cagcccgtca ccaccgccga cattctgacg
     7321 ccacgacgcc gggaggatta cggtaaggac ctgtggagtg cttatcagac cattcaggag
     7381 aatatgctga aaggcggaat ttcaggtcgc agcgctaaag gaaaacgtat ccatacccgt
     7441 gccattcaca gcattgacac tgacattaag ctcaaccgcg cattgtgggt gatggcggaa
     7501 accctgctgg agagcctgcg ctgattcagc cattaccctg aaaaccacgc atttatttgt
     7561 cctttaagga gtcatttcat gaaaaccatt tcacataatt ccaccacatc ttctgtttcc
     7621 gtaaccgctg catcagggaa tgaccagcct cagttggttg ccaccatagt ccctgatgaa
     7681 cagcgcatca gcttctggcc gcagcatttt ggcctcattc cacagtgggt ggccctggag
     7741 ccccgtgttt tcggctggat ggaccgtctg tgcgaagact actgcggtgg tatctggaat
     7801 ctgtacaccc tgaacaacgg cggggcattt atggcccccg aaccggatga cgatgatgac
     7861 gaaacatggg tactgttcaa tgccatgaac ggcaaccgcg ctgaaatgag cccggaagcc
     7921 gctggcattg ctgcctgtct gatgacgtac agtcatcacg cctgtcgtac ggagtgttat
     7981 gccatgacgg tccattatta ccggctgcgg gattacgccc tgcagcatcc ggaatgcagc
     8041 gccattatgc gcatcatcga ctgaatggag gcagggacaa tgcagcagct ttcctttctg
     8101 cccggagaaa tgacgcccgg cgagcgcagt ctcattctgc gggctctgaa aaccctggac
     8161 cgccatcttc atgaacccgg tgtggccttc acctccaccc gtgcggcacg ggaatggctg
     8221 attctgaaca tggcgggact ggagcgtgaa gagttccggg tgctgtatct gaataaccag
     8281 aatcagctga ttgccggtga aaccctcttc accggcacca tcaaccgcac ggaagtccat
     8341 ccccgggaag tgattaaacg cgccctgtac cacaatgccg ctgccgtggt actggcgcac
     8401 aatcacccgt ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg
     8461 gtacaggcac tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
     8521 caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg
     8581 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaattatc actcgtggtg
     8641 aagccatgcg tattcaccaa caacatccga catcccgtct tttcccgttc tgtaccggca
     8701 aataccgctg gcacggcagc actgaagcgt ataccggtcg tgaagtgcag gatattcccg
     8761 gtgtgttggc cgtgtttgct gaacgtcgta aggacagttt tggcccgtat gtccggctga
     8821 tgagcgtcac cctgaactga atcaggacgg gcattcagat gagcagcatt atcaccatca
     8881 cagtgtatac gctggatgaa ctttcctgcc cggcacgggg aaaagcccgc gactggtacc
     8941 gtcagcatca tgctgacagc aactggtatg aaaacgtgta tgaagacttc agggaggtct
     9001 gtggcatttt cgggattgac ctccgccagc gcgttttccg gctcagcaat ggcaggttta
     9061 tggaagagcc gtgcatctgg ttcagcggat tctgcagcca gggggacgga gcctgttttg
     9121 aagggcgctg gcactggcag ccagcgacac cccggaaaat cagggaatat gcccctcagg
     9181 accgtgagct tcatcgtatc gcggataccc tgcaggcggt gcagaaacgt aacttctggc
     9241 agcttcaggc tgaaatcagt caccggggac gttactgcca tccgtacagc atggatatca
     9301 ccgtcacccg gaacagtccg actggtcagg caatgacaac cgatgcggaa gccgctgtca
     9361 gcgaagccct gcgcgacctg gccttctggc tgtaccgcca gctggaaaac aagtacgact
     9421 ggctgacctc agacaccgct gtggatgagg cccttctcat caacgaatat accttcaccg
     9481 aagccggtct gcgcgccggc tgacccgtca tcgttaaact atcattcacc gattttctgt
     9541 gaggatttta tcatgtcgga cacactcccc ggaacaacgc ttcccgacga caacaaagac
     9601 ctcccctggt gggggctgcc ctgcaccgtg acgccctgtt tcggggcctg tctggtgcag
     9661 gagggtaacc ggttgcatta ccttgccgac cgcgccggta tcagaggccg gttcagcgat
     9721 gcggatgcgt accatccgga ccaggccttt ccgctgctga tgaaacaacc ggaactcatg
     9781 ctcaccagcg gtgaactgaa tccccgccat cagcataccg tcacactgta tgcgaaagga
     9841 ctgacctgcg aagccgatac cctgggctcc tgtgattacg tttatctggc tgtttatccg
     9901 acgcccgaaa tgaaaaatta actctccaga atagccttct gctacggcct ggtgttttca
     9961 ccacgccact tttccatttt tatatctgca tatcaggaaa atcttcagta tgaaaacatt
    10021 acccgacaca cacgtacggg cggcatcgtg ctgcccgtct cccgtcacca tctggcagac
    10081 actgctcacc cgactgctgg accagcacta tggcctcacg ctgaatgaca caccgttcgc
    10141 tgatgaacgt gtgattgagc agcatattga ggcagggatt tcactgtgtg acgcggtgaa
    10201 ctttctcgtt gaaaaatacg cgcaggtacg taccgaccag ccgggattca gcgcaggagc
    10261 cccgtcgcag ttaatcaaca gcattgatat tctccgggct cgcagggcga ccggcctgat
    10321 gacccggaac aattacagaa tggtaaataa cattacccag ggcaagcatc cggaggcaaa
    10381 acgatgaaac tggccctgac gctggaagct gacagcgtta acgtacaggc actgaacatg
    10441 gggcgaattg tcgttgacgt cgatggtgtt gagcttgccg aactgattaa cgtggtctgc
    10501 gataatggct actcccttcg tgttgttgat gaatctgacc ggacctcaac agacagcata
    10561 cccccctctg ctgccctgag cggtatacgc tgtagtaccg cgcatatcac ggaaacagac
    10621 aacgcctggc tgtactcgct gtcgcatcag acgaatgaca ccggcgaatc agaatggatt
    10681 cattttacag gtagcggata tctgttacgt accgatgcgt ggtcataccc ggttctgcgg
    10741 cttaaacgcc tggggctgtc aaaaacgttc cgccgtctgg ttgtcacact tacccgacgt
    10801 tatggcgtca gtctcattca tctggatgcc agcactgaat gcctgccgga tttacccact
    10861 ttcgactggt aacccggaaa ttcacaacat gaaatcatta accacggaaa cagcgctgta
    10921 tattctgatt gcgtggctgc aggacaatat tgactgcgaa tcagggatta tcttcgacaa
    10981 cgatgaggat aaaacggatt cagcagcact gctgccctgc attaaacagg ccagggaaga
    11041 tatccgtacc ctgcgccaac tgcagcttct gcaacagaac cggtgagtct tactcaccag
    11101 acttcattcc actgacgcca gcctgaatac ggctggcgtt ttcgtttata aaccaaaagg
    11161 aaaaacgatg atgtcagata tcacaatctc ccgcccggaa ctggtcaacg gacatacgga
    11221 cgttatctgc tcaaccagta tccgccacat tctggctgta cgaaagagtg cgctgctgca
    11281 aatcgacaca cttatccggc aactggcaga aatctcagta ctgacagaaa gtattggagg
    11341 taaaaccgcc ctggactggg cgatgaaaca ggattttcgc tgcggctgct ggctcatgga
    11401 gaaaccggaa accgcaatga aagccatcac ccgcaatctc gatcgcgaaa tctggcgtga
    11461 cctgatgcaa cattccggga tgctgtcatt aatggatgcg caggcccatg atacatggta
    11521 ccgctcactg gagtacgata actttccgga aatcagcgag gcgaatatcc tgagtacgtt
    11581 tgaacaactg caccagaata aggatgacgt gtttgagcga ggtgtgatca acctcttcag
    11641 agggctgagc tggaattaca aaaccaattg cccctgtaaa tttggcaata aaattatcgt
    11701 caacaatctg gtgagatggg accgatgggg attgcatctt atcaccgggc aacaggcaga
    11761 tcgactcgct gacctggaaa gaatgctgca tctgttcagc ggcaaaccga tccccgacag
    11821 ccgggaaaat atcatcattc ggctggatga tcacatccgc tccgttcagg gcaaagcgtg
    11881 ctatgaagat gagatgttca gcatcagata ctttaagaag ggctccgcac acatcacgtt
    11941 caggaaacca gaactggttg acaggctaaa tgacataatt gccaggcact atccaaatgt
    12001 attagcagac aggtaatgtg gccttgcccc gctattaaac atgtcatatt tttgctatca
    12061 ttgtgaaaat ttttcggagg aaacatgact acaaaagacc gtattcagtc ccgactgaac
    12121 cgttcaaaac gttatgtgtt cacccgggat gatttcaggg atatcgctgg ctatgatcag
    12181 gttggcagag ctcttcgtac actggtaaac gaaggtaagt tgatgaaagt gggctatggt
    12241 gtttacacca aagcccgtcg taacatcatc actgggaaaa tcatgcccgc agcgcctggg
    12301 ggctctgctg cagtaattac agaggccctg gagcgcctga atatcagata ccgtctggca
    12361 ggagcaacgg aatgctacaa taaaagtcag tccacacaaa ttcctgtatc tctggggata
    12421 aaaacacctt ctcgatttaa aagaatgtta atagtaggga aaagtaaaat caatgcctga
    12481 attaacatca ttatttcctg acgttgccga tgcactgg
//
LOCUS       DACADA010000130        12498 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.132, whole genome shotgun sequence.
ACCESSION   DACADA010000130 DACADA010000000
VERSION     DACADA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.132"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(92..643)
                     /gene="ybcL"
                     /locus_tag="ISB17_003606"
     CDS             complement(92..643)
                     /gene="ybcL"
                     /locus_tag="ISB17_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAP1981497.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(812..1144)
                     /gene="emrE"
                     /locus_tag="ISB17_003607"
     CDS             complement(812..1144)
                     /gene="emrE"
                     /locus_tag="ISB17_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAP1981498.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1488..1802)
                     /gene="fliE"
                     /locus_tag="ISB17_003608"
     CDS             complement(1488..1802)
                     /gene="fliE"
                     /locus_tag="ISB17_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP1981499.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2017..3675
                     /gene="fliF"
                     /locus_tag="ISB17_003609"
     CDS             2017..3675
                     /gene="fliF"
                     /locus_tag="ISB17_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAP1981500.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEMEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3668..4663
                     /gene="fliG"
                     /locus_tag="ISB17_003610"
     CDS             3668..4663
                     /gene="fliG"
                     /locus_tag="ISB17_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP1981501.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4656..5342
                     /gene="fliH"
                     /locus_tag="ISB17_003611"
     CDS             4656..5342
                     /gene="fliH"
                     /locus_tag="ISB17_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAP1981502.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5342..6715
                     /gene="fliI"
                     /locus_tag="ISB17_003612"
     CDS             5342..6715
                     /gene="fliI"
                     /locus_tag="ISB17_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAP1981503.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSKTHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6734..7177
                     /gene="fliJ"
                     /locus_tag="ISB17_003613"
     CDS             6734..7177
                     /gene="fliJ"
                     /locus_tag="ISB17_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAP1981504.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7174..8301
                     /gene="fliK"
                     /locus_tag="ISB17_003614"
     CDS             7174..8301
                     /gene="fliK"
                     /locus_tag="ISB17_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAP1981505.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8406..8870
                     /gene="fliL"
                     /locus_tag="ISB17_003615"
     CDS             8406..8870
                     /gene="fliL"
                     /locus_tag="ISB17_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAP1981506.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8875..9879
                     /gene="fliM"
                     /locus_tag="ISB17_003616"
     CDS             8875..9879
                     /gene="fliM"
                     /locus_tag="ISB17_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAP1981507.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9876..10289
                     /gene="fliN"
                     /locus_tag="ISB17_003617"
     CDS             9876..10289
                     /gene="fliN"
                     /locus_tag="ISB17_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAP1981508.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10292..10657
                     /gene="fliO"
                     /locus_tag="ISB17_003618"
     CDS             10292..10657
                     /gene="fliO"
                     /locus_tag="ISB17_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAP1981509.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10657..11394
                     /gene="fliP"
                     /locus_tag="ISB17_003619"
     CDS             10657..11394
                     /gene="fliP"
                     /locus_tag="ISB17_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP1981510.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11404..11673
                     /gene="fliQ"
                     /locus_tag="ISB17_003620"
     CDS             11404..11673
                     /gene="fliQ"
                     /locus_tag="ISB17_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAP1981511.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11682..12467
                     /gene="fliR"
                     /locus_tag="ISB17_003621"
     CDS             11682..12467
                     /gene="fliR"
                     /locus_tag="ISB17_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAP1981512.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
BASE COUNT         3046 a         3283 c         3315 g         2854 t
ORIGIN      
        1 gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca atctttaggt
       61 tagtcttatc ttttgccagc atactcttac cctactttat ctcataaact ggtgttatct
      121 cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg ctggaattag
      181 aatccacagg aattttatca gtttttagag cccatacttt aaactggtaa tgatggggtt
      241 tatctccttt gggaggacat gctccaccaa atcctgcata accaaaatca tttcggcctt
      301 gaacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagcg ggcaaatatg
      361 ttacagttgc tggaatatta gcaacagtcc agtgccacca tccactgcct gtaggagcat
      421 ctggatcata cacagttacg gcaaagcttt tggtgccttc aggagcacca gaccaggtta
      481 atgagggcga tgtattacca ccttcacacc caaatccaga aaatacatga gacgttgtaa
      541 gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc tgcgcagaaa
      601 atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc tcattgttgt
      661 gacctatcgt tattttattt gatgtgcctt gatctcatta tgcataaagg cgcaatgatc
      721 gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt agccataata tgcacaaatc
      781 agattcctgt tatctcaagg ataattattt tttaatgcgg tgcacttcgt gacaacaagt
      841 taatgactaa cacaccggca caaatcaaca tcatgcctat gatagctggc aggtccagtc
      901 gttgaccaaa aaatccccag gaaagcaaac taatcagaac gataccgact cctgaccaga
      961 tagcataagc aatccctgta ggtatataag ccagcgtctg agctaataac cagaatgatg
     1021 cacaatagca aataattgtg ccaacagagg gccataaccg tgtaaacccc tctgaaaact
     1081 tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc accaagataa atataagggt
     1141 tcatagcata ttctttcctg ttcatactgg agagaattgt actacagttt gaacacaact
     1201 cactgttttc atcctggtgt atgtgtatcc agggggctct ggcgtggcaa gagataaagg
     1261 caggaacaat aatgatcaga aggaacccca ggctaacgaa agagttcact cttcctggga
     1321 gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag aaaacaacca
     1381 ggatcaggta tccattttaa accagaaatt gagtgagttt gcccatccat gccccacgct
     1441 gtgggatcgg atataattaa acaagcatag tcaacaggtt acacaaccta cacctgcatg
     1501 gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata
     1561 gaaactgagg ctttttgcat atcggtcatc acatcgttta gcgccacgcc gggttcaccg
     1621 agggtgaatt tttctgcctg cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc
     1681 gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgattc ctgcgcacgc
     1741 gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac
     1801 attttcgtct cccggataat ttctgatagc aaagcctacc agtaagtcaa taagacaaag
     1861 gcgctaaata gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa
     1921 taatgggagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc
     1981 caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca
     2041 aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttgcc
     2101 ggttccgcag cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac
     2161 cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg
     2221 caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaggt tccggcagat
     2281 aaagttcacg aactgcgcct gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
     2341 ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
     2401 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
     2461 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
     2521 ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt
     2581 agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgccagg aaacgtcacg
     2641 ctggtggatc agggcgggca tctgttaacc cagtccaata ccagcgggcg cgatcttaat
     2701 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg tattgaagcg
     2761 atcctgtcgc ctattgttgg taacggtaat attcacgccc aggtcacggc gcaactggac
     2821 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
     2881 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
     2941 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcaccaat cagcacgcct
     3001 ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag tgggccgcgt
     3061 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
     3121 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg
     3181 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agctctgacc
     3241 cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt taactcgccg
     3301 ttcaatagca gtgacgaaag cggcggagca ctgccgttct ggcaacagca agtgtttatc
     3361 gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gttgctgtgg
     3421 cggaaagcgg tacgcccgca gctaacacgt cgcgccgagg cggtgaagac tgtgcagcaa
     3481 caagcgctgg cccgcgagga aatggaagat gcggtggaag tccgtctgag caaagacgaa
     3541 caactccaac aacggcgcgc taaccaacga ctgggggcag aagtcatgag ccagcgtatc
     3601 cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat
     3661 aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg
     3721 cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaacccttag
     3781 cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga
     3841 gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg
     3901 ttcggtattg gtcaaagctc tgggtgaaga acgtgccgcc agcctgctgg aagatattct
     3961 cgaaacccgc gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagcgc
     4021 cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa
     4081 gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat
     4141 gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt
     4201 actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac
     4261 ggcagccgaa attatcaacc tgatgaaaac gcagcaggaa gaagccgtta ttaccgccgt
     4321 gcgtgaattc gacggcgagc tggcgcagaa aatcatcgac gagatgttcc tgttcgagaa
     4381 tctggtggat gtcgatgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc
     4441 gctgctgatc gcgctgaaag gggccgagca gccactgcgc gagaaattct tgcgcaatat
     4501 gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct
     4561 gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgtc ttgccgaaac
     4621 tggcgagatg gtgattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa
     4681 cctggacgcc ggacgatctc gcgccaccac cggcagagtt tgtgccaatg gtcgagtcgg
     4741 aagaaaccat tattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa
     4801 tgcaggccca cgagcagggt tatcaggcag ggattgccga aggtcgccag caaggtcatg
     4861 aacagggcta tcaggaagga ctggcccagg ggctggaaca aggtctggca gaggcgaagg
     4921 ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc
     4981 ttgatgcgct tgatagtgtg atagcttcgc gcctgatgca gatggcgctg gaggcagcac
     5041 gtcaggtcat aggtcagacg ccaacggtgg ataactcggc actgatcaaa cagatccaac
     5101 agttgttgca gcaagaaccg ttattcagcg gtaaaccaca gctgcgcgta cacccggatg
     5161 atctgcaacg tgttgatgat atgctcggcg caactttaag tttgcatggc tggcgcttgc
     5221 ggggcgatcc caccctccat cctggcggtt gcaaagtctc cgccgatgaa ggcgatctcg
     5281 acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt
     5341 aatgaccacg cgcctgactc gctggctaac cacgctagat aactttgaag ccaaaatggc
     5401 gcagttgcct gcggtacgtc gctacgggcg attaacccgc gctaccgggc tggtgctgga
     5461 agccaccgga ttacaattgc cgctcggcgc aacctgtgtc attgagcgcc agaacggcag
     5521 caaaacgcac gaagtagaaa gcgaagtcgt cggctttaac ggtcaacggc tgtttttaat
     5581 gccgctggag gaagtggaag gtgtcctgcc cggcgcgcgt gtatatgcca aaaacatttc
     5641 ggcagaaggg ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat taggtcgtgt
     5701 tctggacggc agcggtaaac cgctcgatgg cctcccctcc cccgatacga cggaaaccgg
     5761 tgcgctgatt accccgccat ttaacccatt gcaacgtaca ccgattgaac atgtgctgga
     5821 caccggcgtg cgcccaatca atgccctgct taccgtcggg cgcgggcagc gtatggggct
     5881 gtttgccggg tccggcgttg gtaaaagtgt gctgttgggg atgatggcac gttacacccg
     5941 cgccgatgtc attgtcgtgg gtttgattgg tgaacgtggg cgcgaagtaa aagattttat
     6001 tgagaatatc ctcggtgccg aaggacgtgc acgctcagtg gtgattgccg ctccggcgga
     6061 tgtttctccg ctcctgcgaa tgcaaggtgc cgcctatgcc acgcgcattg ccgaagattt
     6121 tcgcgatcgt ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc
     6181 ccagcgtgaa attgcgctgg cgattggcga accacctgct actaaaggtt atccaccgtc
     6241 ggtgtttgcc aaattaccgg cactggtcga gcgtgccgga aatggcatta gcggcggcgg
     6301 ctcgattacc gcgttttata ccgtgctcac cgagggcgat gaccagcagg accccattgc
     6361 cgactccgcg cgggccatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc
     6421 cgggcactat ccggctatcg atattgaagc gtcgatcagc cgcgcaatga cggcgttgat
     6481 cagtgagcaa cattacgcac gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg
     6541 taaccgagat ctggttagcg tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa
     6601 agccatcgcc ctgtggccgc agctggaggg ctatttgcaa caaggcattt ttgaacgcgc
     6661 ggactgggaa gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag
     6721 gagataacgg cagatggcag aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa
     6781 agaggtagag gatgccgcgc gcctgctggg tgaaatgcgt cgcggatgtc agcaggcgga
     6841 agaacagctc aaaatgctga tcgattatca gaatgaatat cgcaataacc tcaatagcga
     6901 tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct
     6961 ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat
     7021 tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga
     7081 acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga
     7141 gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttaattacc
     7201 gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt
     7261 ctcgcgttgt tgagcgaagc attagcaggc gagacaacta ccgacaaagc ggccccccag
     7321 ttgctggtgg caacagataa gcccacgaca aaaggcgagc cgctgatcag cgatattgtt
     7381 tccgacgcgc aacaagcgga tttactgatc cccgtggatg aaacactgcc tgtcatcaac
     7441 gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt ggctgcggtg
     7501 gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca
     7561 agcctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat
     7621 gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgcttt tcacaaaact gacttctgcg
     7681 caactcacaa cagcacagcc tgatgacgcc cccggcacac cagctcagcc attaacaccg
     7741 ctggtagcag aagcccagag taaagcggaa gtcatcagca caccttcgcc ggtgactgct
     7801 gccgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct
     7861 gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattaagcca gcatatttcg
     7921 ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccaca ggatttaggt
     7981 gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc agatccagat gatttcaccg
     8041 catcagcacg tacgcgcagc cctggaagca gcgctgccgg tactgcgtac gcagctggcc
     8101 gaaagtggca ttcagttagg gcaaagcaac attagtggcg aaagctttag tggtcagcag
     8161 caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctgacgggg
     8221 gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggcaacagc
     8281 ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct
     8341 ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc
     8401 aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc
     8461 tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc
     8521 aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc
     8581 tggatacctt tacggtcaat ttgggcgatg cggatcgcgt gctttatatc ggcataaccc
     8641 tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta
     8701 gtcgcttgct gttactgttt tcacgtcagg atgctgccgt actggcgaca gaagaaggca
     8761 agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac
     8821 cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc
     8881 gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa
     8941 gacgaaccga cagccagtgt tagcggcgaa agtgacattc gtccgtacga tccgaatacc
     9001 caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc
     9061 cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggacat taccgtcggg
     9121 gccatccgca ttcagccgta ccatgaattt gcccgcaact tgccggtgcc gaccaacctg
     9181 aaccttatcc accttaaacc gctgcgcggc acagggctgg tggtgttctc gccgagcctg
     9241 gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa
     9301 ggtcgcgagt ttactcatac cgaacagcgc gtcatcaacc gcatgttaaa gctggcgctt
     9361 gaaggctata gcgacgcctg gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg
     9421 gaaatgcagg tgaaatttac caatatcacc acctcgccga acgacattgt ggttaacacg
     9481 ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc
     9541 atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa
     9601 gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg
     9661 gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac
     9721 gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt cccggttctg
     9781 accagtcagt acggcactct caacggtcag tatgcgttac ggatagaaca tttgattaac
     9841 ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg
     9901 atgacaacaa cggcgcaatg gacgatatgt gggctgaagc gttgagcgag caaaaatcaa
     9961 ccagcggaaa aagcgctgcc gacgcaatgt tccagcaatt tggcggcgga gatgtcagcg
    10021 gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg
    10081 gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaaggatca gtcgtggcgc
    10141 tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta atcgcccagg
    10201 gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat caccgatatc attaccccgt
    10261 ccgagcggat gcgccgcctg agccgttagt gatgaataac catgctactg tgcaatcttc
    10321 cgcgccggtt tctgctgcgc cactgctgca ggtgagcggc gcactcatcg ccattattgc
    10381 cctgatcctc gctgccgcct ggctggtaaa acggctggga tttgccccta aacgcactgg
    10441 cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt
    10501 ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct
    10561 acataaactt cccccttcag caccaacgga ggagataccg cagaccgatt ttcagtcggt
    10621 catgaaaaat ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca
    10681 ccggtccttc tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc
    10741 cagccgctac ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc
    10801 acctcgttga cgttcattcc agcgatttta ctgatgatga ccagtttcac ccgcatcatt
    10861 attgtttttg gtttattgcg taacgcgctg ggaacgccct ccgcgccacc taaccaggta
    10921 ttgctgggac tggcactgtt tttgaccttt tttattatgt cgccagtgat cgacaaaatt
    10981 tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct caatgcagga ggcgctggaa
    11041 aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc ggatttaggg
    11101 ttgtttgcca gactggcgaa taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc
    11161 attttgctcc cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg
    11221 attttcatcc cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg
    11281 atgatgatgg ttcccccagc caccattgct ctgcccttta aactgatgct gtttgtactg
    11341 gtggatggct ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc
    11401 aaaatgacac ctgaatcggt catgatgatg gggactgaag cgatgaaagt cgcgctggca
    11461 ctggctgccc cactgttgct ggtggcgctg gtcacgggtc ttatcatcag tattttgcag
    11521 gccgccacgc agatcaacga aatgacgctg tcgtttattc caaaaatcat cgccgtattt
    11581 atcgccatta ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc
    11641 ttgttcacta acctgccgta tatcatcggg taggccgtac aatgatgcag gtaacaagcg
    11701 atcaatggct ttcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctca
    11761 tttctaccgc gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg
    11821 caatgatgat cacgttcgcc attgccccat cattacctgc caacgatgtt cctgtttttt
    11881 cgttctttgc tctgtggctg gccgtgcagc agatcctgat cggcattgcg cttggtttta
    11941 ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg
    12001 ggctgtcatt tgcgacattt gtcgatcctg gcagccatct caatatgccc gttttagcgc
    12061 gcatcatgga tatgctggca ttactgctgt tcctgacatt taacggtcat ttatggttga
    12121 tttcactgct ggtcgatacc tttcacaccc tgccgattgg tagcgaaccg ttgaacagca
    12181 atgcgtttct ggcactcacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg
    12241 cgttaccgct cattactctg ttgctgaccc tgaatctggc attaggttta ctgaatcgta
    12301 tggccccgca attatccatt tttgttattg gatttccatt aactctgact gtcggcatct
    12361 ctttaatggc ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaaa
    12421 tttttaattt gctggctgat attattagtg aattaccatt aatataattc cgtaacgttt
    12481 atcatgctac cctaagga
//
LOCUS       DACADA010000131        12457 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.133, whole genome shotgun sequence.
ACCESSION   DACADA010000131 DACADA010000000
VERSION     DACADA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.133"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(66..839)
                     /gene="uxuR"
                     /locus_tag="ISB17_003622"
     CDS             complement(66..839)
                     /gene="uxuR"
                     /locus_tag="ISB17_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAP1981513.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1054..2514)
                     /locus_tag="ISB17_003623"
     CDS             complement(1054..2514)
                     /locus_tag="ISB17_003623"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP1981514.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2595..3779)
                     /gene="uxuA"
                     /locus_tag="ISB17_003624"
     CDS             complement(2595..3779)
                     /gene="uxuA"
                     /locus_tag="ISB17_003624"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP1981515.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4119..5462
                     /gene="gntP"
                     /locus_tag="ISB17_003625"
     CDS             4119..5462
                     /gene="gntP"
                     /locus_tag="ISB17_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAP1981516.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5636..6538)
                     /gene="fimH"
                     /locus_tag="ISB17_003626"
     CDS             complement(5636..6538)
                     /gene="fimH"
                     /locus_tag="ISB17_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAP1981517.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSAHDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6558..7061)
                     /gene="fimG"
                     /locus_tag="ISB17_003627"
     CDS             complement(6558..7061)
                     /gene="fimG"
                     /locus_tag="ISB17_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAP1981518.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATFDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7074..7604)
                     /gene="fimF"
                     /locus_tag="ISB17_003628"
     CDS             complement(7074..7604)
                     /gene="fimF"
                     /locus_tag="ISB17_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAP1981519.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNDIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7614..10250)
                     /gene="fimD"
                     /locus_tag="ISB17_003629"
     CDS             complement(7614..10250)
                     /gene="fimD"
                     /locus_tag="ISB17_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAP1981520.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10317..11042)
                     /gene="fimC"
                     /locus_tag="ISB17_003630"
     CDS             complement(10317..11042)
                     /gene="fimC"
                     /locus_tag="ISB17_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAP1981521.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11079..11618)
                     /locus_tag="ISB17_003631"
     CDS             complement(11079..11618)
                     /locus_tag="ISB17_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1981522.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11683..12231)
                     /gene="fimA"
                     /locus_tag="ISB17_003632"
     CDS             complement(11683..12231)
                     /gene="fimA"
                     /locus_tag="ISB17_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/fibrin-binding adhesin FimA"
                     /protein_id="HAP1981523.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2934 a         3282 c         3046 g         3195 t
ORIGIN      
        1 ctcattgcga cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata
       61 ataagtcagg cgtcgacttt atccagcggc catgaatcaa acagatagcc atcaaaataa
      121 atatcgtcaa cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac
      181 attgccagct tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga
      241 tcgcccaacc actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac
      301 attggattgt tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta
      361 tgcgttgctt cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc
      421 gcactggaag ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct
      481 tcgcgggtag cctgcagggc ggcaaactcg gcgatgttgc tctcaagaag ctgtcgcgcc
      541 tgcaacagct caaaaggacc cgcatcattg caaacgttgg catccggact gtctgtgttg
      601 tggctgcctg agctatcaag aacatagata ccggcacccc ggcgcacttc caccagccct
      661 ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct
      721 gcaatttcac gctccggcgg caaccgttcg ccaggattgt acggcgtctt tatgatcaga
      781 tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg
      841 gtgttgtgtc cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta
      901 tccggcctac aacgggcaat tttacggcac ccgttaatct caaactatac cattagcact
      961 ggaagttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg
     1021 attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc
     1081 accgcgttcg cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt cggcattctg
     1141 cggcagatca tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc
     1201 ctgatactgc gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc
     1261 attaccctgc tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag
     1321 gtgacgccag ctgccgccgt tttgcaggtg cagacgcacc ggatccagca gacgctgcgg
     1381 taacttctgg ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct
     1441 gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag
     1501 cgttggcgct tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt
     1561 catggtgtcg gcaatagttt catagccgcc gaggtaaccg aggtatgcca ggaaagagtg
     1621 gctgccgttc agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg
     1681 tgcgcctact ttatcccagt ccggacgacc attaacgaag ttatcttcaa tcacccactg
     1741 acggaacggt tcgcaggcaa tggcgcacgg gtcataaaca cccagttggt cagcaatttc
     1801 ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt
     1861 gacgttctct tcaatccatg ccgccagctg cgggtcacgc gcctgagcca gccccagtac
     1921 cgcgaccttc gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaacgc
     1981 tttcagccct ttttcacgac gcagacgcag ggcttcgacg atgtaaccaa tcgcggactt
     2041 cggcgcagtc gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc
     2101 gcttgccgca tcagcgcagt agcctttttc cgtgaccgtc agagagacaa tcgccgtttg
     2161 cggacgcgcc atcgcgttga gaataccttc gcagccgtcg atttccgggt gcagcgcttc
     2221 tttcatcgaa ccgataattt tcagctcggt gctttccgcg cctttttccg ccacggtgta
     2281 cagcagttgc tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt
     2341 aacttcgcag ataccccagt cgctgtcggt gctttccagc agatgatggg tgtacagcgc
     2401 ctggtgcgca cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga
     2461 atgatcccat gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa
     2521 ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc
     2581 catgccggtg gatattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga
     2641 acttcggcca ggcctttcag acgaccaatt gcggagtaac ctgggttggt tttcttcttc
     2701 aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct
     2761 tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg
     2821 tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag
     2881 cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg
     2941 tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat gctatttacg
     3001 gtatcaacca tccactgcat atcttcaatg gtagaaacaa tgcgcggcag gccgaggatt
     3061 gggcgcggcg gatcgtccgg gtgaacagcc atacgcacgc caacttcttc ggcaaccgga
     3121 ataatcgcct tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc gatatctttg
     3181 tacagctcca ggtgcttacg gaactggtcg agggtgtacc cttcttccgc gcccggcaga
     3241 ccggcaatga tgttacgggt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt
     3301 tcagctgcct gagcaatttc ttcttcggtg taatccgctt ccgcacctgg gcgtttcagg
     3361 atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct
     3421 ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac
     3481 acggtgcgaa taccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac
     3541 tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggcacgct ttctacgaca
     3601 gaccacacca ggcctgcatc ttcaacgata gccttgcgtt tgaggatctc ttcaacggac
     3661 catacttcgc cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga
     3721 cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg
     3781 atttttcctc tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt
     3841 taggtatccg gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta
     3901 taagttctac cttttggtaa tacatgtcaa cccgaattct aaaattggtt aaccacatca
     3961 caagaatttc actctcctgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat
     4021 tgatcagagg atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc
     4081 caaaataaca acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct
     4141 gggtggtatt cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa
     4201 tggtggcttt gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt
     4261 cgctgctgca caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg
     4321 tggtgttcgg tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag
     4381 cacatacgct gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg
     4441 gcctgatttt tggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg
     4501 ttattgtcat tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag
     4561 cagctgccac taccgcacat tcactgttcc caccgcagcc gggtcctgtg gcgctggtga
     4621 atgcttatgg cgcagatatg gggatggttt atatctatgg cgtactggtg acgatcccaa
     4681 gtgtaatctg cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc
     4741 catcattcct gaaagcagat caaccggtag atatgaacaa tctgccctct ttcggcgttt
     4801 cgattctggt gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct
     4861 ggctggtaaa agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg
     4921 caatgtttat tgcgatggtg gttgcattcg tactctttgg caccgcacgt ggtcatgaca
     4981 tgcagtgggt gatgaacgct tttgaaagtg cggtgaaaag cattgcaatg gtgattctga
     5041 tcatcggtgc gggtggcgtg ttgaagcaaa ctatcatcga caccggcatt ggcgacacca
     5101 tcggcatgtt gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg
     5161 tgctaattcg tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta
     5221 tcagtgctgc aatccttgat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc
     5281 tggtactggc gacggctgcg ggttccaaca ccctcaccca cattaacgat gcatctttct
     5341 ggttgttcaa aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc
     5401 tggagctggt caactccgtg gttgggctga ttattgtgtt gattatcagc atggtagcgt
     5461 aaaagagcac ctgagcctgc cattggcagg ctctgttcgg attgtcggca aagtgcactt
     5521 tgtttatgcc agatgcgacg ctggcgcgtc ttatctggcc tacaaagggc taacgtgcag
     5581 ttttttagct tcaggcaata ttgcgtacct gcattagcaa tgttctgtga tttctttatt
     5641 gataaacaaa agtcacgcca ataatcgatt gcacattccc tgcagtcacc tgccctccgg
     5701 tacgtgcgta atttgccgtt aatcccagac ttaccgccga agtccctact gctcctaacg
     5761 ataccgtgtt attcgctgga ataatcgtac cgttgcgcgt caactgtacg ccgacgccct
     5821 gcgctggtga aaacgacgcg gtattggtga aaatcgagtt gcccgcatct gcggttgtgc
     5881 cggagaggta ataccccagg ttttggcttt tcgcacaata aacggtaaga ggaattggca
     5941 ctgaaccagg gtagtccggc agagtaacgg tgacatcatg agcagaaaca tcgcagccgc
     6001 cagtaggcac caccacatca ttattggcgt aaatattcca cacaaactgg aaatcatcgc
     6061 tgttatagtt gttggtctgt cgcaaaataa gcacggcaat taatgagcca gctttaatcg
     6121 ccaccccacc cgcactgctc acaggcgtca aataaagcgc caccggccac ggcttatccg
     6181 ttctcgaatt ataaacaacc cgcggcgttt cgctggtagt cggaaatgga tagctactgc
     6241 cactatattt tacggtcccg gaaaaattag ataacacgcc gccataagcc gagcctcgtt
     6301 gcagtgtgac atagtctgta atggtttccg gataatcgtt atggcaaaag atttgcgtcg
     6361 aaagatctac gaccaggttt tgccccacat tcacggcagg cgcaaggttt acataaacat
     6421 tagcgctgcc accgccaata ggaatagcgg taccattggc ggttttacag gcgaatgacc
     6481 aggcatttac cgaccagccc atcagcagta cagcaaacag ggtaataact cgtttcatga
     6541 caattatctc ttcgggttca gctgtaggta taggtgatgc taatcactgc ctgaatggtt
     6601 ccctgagtgg ctccgccatt taccgtcaat gctctgacct gtaacgggaa gtgcgctgat
     6661 tgtgaggaat catccacctg aactgttttg gttgcgccag tattcaatgt gttgccactg
     6721 tcatcctgta gctctaactg gatgttttgc gcggtcccct ggtttttata atatccggta
     6781 ctgtcggctg ccccgctgaa gctggcagtg accctcgacg ttcccaccgg acaattagtc
     6841 aactcaagcg caacatcatg ccaggccgat gccgccccgg cagacatcag actgaaagaa
     6901 taaagatcgc cgagatcaaa cgtggcattg gtggtggaaa ccgtgcacgg tttggcgacg
     6961 accttaccgt tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc
     7021 aatattgccg ccaataaata cccacgtttg cgccatttca tgagcatctc cagttactga
     7081 tattcaagag tgaaggtagc cgtggcattg atatgccccg cagtgacagg cacctgtgtc
     7141 gccattagcc gggcgtaaaa attcagcgta tttggtttac ccggcgtcag ggtcgtccac
     7201 gaaagcgcgg acgatggagc attaaggggt atttgatttt gctgctcatt cagaagctgt
     7261 attcccagtc ccgaagccgc tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg
     7321 ctatctgcaa caccggtaaa cccaactttt acggcagaaa cggcattacc acagggtgac
     7381 agcaaaatac gaaatggaac gacaggagtc gtcgcgccaa tgtcgttaaa ttgcttcgcc
     7441 gcgttttcca tcaaatcaac agtaaaattg gttgattcag cggccacact acagccgtta
     7501 tctctgacat agccgcggat agtaatcgtg ctatccgcag ccaaaacgcg acttaccgcc
     7561 agccacaaaa aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga
     7621 cattcagctg atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg
     7681 gcgatacagt gagcattttc ctcttctccc catttcacct gaacttttcc cgctaaaggc
     7741 attccgctga ggtaaacctg accattatcc gcaacaatgc cgctactctg gctactctct
     7801 gatgtcacca tcgccccaaa cggcagcggc ttattattgt gggttagcgt catgagcagt
     7861 tttatcccaa cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg
     7921 ttagcgaccg cgttatctaa atcgacgtta tcagccaggg tattggtatc cagcgccact
     7981 ctgttttccc gatattcggt ggcataaggc agcacggcat aaccgcgcca gtcggtacgc
     8041 acccccgtct ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagtaccacc
     8101 gtatcgttta acggctgccc cagcgttacg ccattggcat gagccagtac cccaccgctg
     8161 actccgtaat agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg
     8221 taaccaccgc gataattcag cgtggcgtag cctgtacttc cgctatttcc atcgcctccc
     8281 ccggcatagc cggtttgcac gctatagctg aggttgttgt cttccagcaa cgtaccgtat
     8341 acaccagcca gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg
     8401 gcatgtcgcc actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg
     8461 ttaagcgcta acatctgatc ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg
     8521 agcgtccagt tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca
     8581 ttactcgttc cccaataagt ttgatggcta ccactcaaat acagtgttga tgagcgcccg
     8641 agttgctgag taacggtgag ttgtaatttc ccgcgtttgt tataagcgag gttgtaatag
     8701 tcggtgaatt tcggcttaac ctgaataaca ccatcctgtg tttcgatgtt gtagccattc
     8761 attcgactgt atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc
     8821 actaactgaa tattcgtgcc tgactcattg agcgatttgt tatagagaaa acgcaccgat
     8881 tgtccgtcat gctgactgtc atcgggaagt gtggaattag cctgcgtcat atccacagac
     8941 agagcaccca gcgcccccat gtttttcccg ataccgaaat taaaagcacg ataacgatcc
     9001 gccagttgcg ttccaccata tattgtccag ccagccggaa ggccgtggag taatgtactc
     9061 tggaaaaagc ggggtttttc ctgttgcgca tttccactac ggtattctcc tgccgtaatg
     9121 gaataacgag tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa
     9181 atctgcgtgc tgccgtcagc ctctttgatc gttacctgca agtcaccact attacctgcg
     9241 gcatagatat cgttgatggt aaaaggcccc ggtggcaccg tactattata aatgtcatac
     9301 ccattttgtt taatagtgac ctgtgcagta ccacgagcaa taccgtggat caccggggca
     9361 aatcctcttt gactatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag
     9421 ttaataccat cgaaaatatc gccctgagta taaccatcac ccagcgtcag ccgggaacgt
     9481 aacggtatta tgtctcgctc aagccaggta ttgatatgct gccatttatt tttgctacct
     9541 gatgatctgt cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca
     9601 atatttaacc cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc
     9661 tgtacactat ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat
     9721 aactcaggag gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg
     9781 ttgagtcgct gctgaccaac atctaaatgc gcagtagcgt cctggaccat tgtggttaat
     9841 ggcacacagg catcatccgc cagcagattc ataccggcga cagaagccat attcagcccc
     9901 atactggcga gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta
     9961 ttaaatgtga catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac
    10021 gtccctggcg gtaattcttg tccattttca aaacgcgata aatcagccac agcctgggga
    10081 tcatccgcta aaaagcgcgg gttaaaatag agttcggcag atgacaaagg tgcctgtgcg
    10141 gcaaaagcac aggcaacaaa gagccggaca aaaaaaccag ccaaacgatg cttacgaata
    10201 tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg
    10261 gtttaaaatc acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat
    10321 tccattacgc ccgtcatttt gggggtaagt gcgccataat catttattgt tcggtaagta
    10381 atattgcttc ctgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaat
    10441 gcattttcaa gaacccgggt tccggcattc aactctgtta ccgtcaggta atagggtgtc
    10501 gggttaatca gcgtcagaga attcgcgcta cgacgaaatc ttaatttttc tgcggcctga
    10561 tcgggtggca acgctaattt agccggacga tagtacagtt taatgcggct gataattgcg
    10621 agctgtagcg tattctcagt caattttgat ttatccattg acggaatcgc tttaacgttc
    10681 atccagaata aactttcccg gtcctgtggc aattggttat ttgttgcatc aagaatacgc
    10741 aaggtattct ctttttttcc cttcatcgca aacagaggag gcgtcacgat aaaacgacca
    10801 tcctttacac catcggcatt ttccacccat gattgaatta aataggtgct attttcatca
    10861 ttatttgtca cggcaagttg cacttgtttt tgccctgccg gataaattac gcgagtcgca
    10921 cctaaggcca ctcccgcttc agcgcgtccg gcaaccatca ttgccatgaa catcaggata
    10981 cctgccagca agcagaatgt tatttcctgc gatttcctta cattgacgtt tttattactc
    11041 actgtcctgt tcctgttatc acattatctg ctgaacaatt actgataggt taaagagaac
    11101 caggcctgtg cattggcgat gccgccagta acccgacgcc cggtagcacg atatttggcg
    11161 atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg aggacgatta
    11221 atcggtacga ggtttccttc atcatcaaac aacgctacgc caatattggt ggctatccct
    11281 ggcccctctc ccacggaaag cacatccgga tttttaccat ccgcgacacc gtgaaacgcc
    11341 acacccacac gttcactcac caccgtgcta cattcccgta aatgaataac aaaaggcacc
    11401 ggtgcgctat cttcccctac cgcgtgaaac cggttactgc tgatttgccc catattgacc
    11461 gtcatttgtt tatcaccagc ttcaatccgg caagtttccg caataatgac gccctgaaat
    11521 tgcatatttc cgccgggcag cgtggtattc catttatttc cggccagagc aaacattggc
    11581 agcaacgacg ctaatagaaa taatctcttc cttttcatcg ttatttttat cacacaaggg
    11641 tgggcatccc tgcccgtaat gacgtccctg aacctgggta ggttattgat actgaacctt
    11701 gaaggtcgca tccgcattag cagcacccgg ggttgcggca ccggttgcaa aataacgcgc
    11761 ctggaacgga atggtgttgg ttccgttatt cagggttgtt tctgaactaa atgtcgcacc
    11821 gtccagcgcc agcgcagcac ccgttctgtc caggatctgc acaccaacgt ttgttgcgct
    11881 acccgcagct gaactctgca gagccagtac atcagtatgc gtagcatcaa ttgccgtacc
    11941 taagaaggca acagcggctt ttgtggcaac agtggtatcg caatcattca gctgaatgtt
    12001 aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg ctagcggtac gaacctggcc
    12061 taactgaacg gtttgatcaa cagagcctgc atcaactgcg caagcggcgt taacaacttc
    12121 ccctttaaag tgaacggtcc caccatttac cgtcgtagta tcggccagag ccgctgcgga
    12181 actgagggac agagccgaca gaacaacgat tgccagagtt ttaattttca tgctgctttc
    12241 ctttaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa
    12301 cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt tggggccatt
    12361 ttgactcata gaggaaagca tcgcggacaa actttttcag tttatttgtt ggcttaatat
    12421 tctattgtta tctttattta tagatgttta tattgca
//
LOCUS       DACADA010000132        12374 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.134, whole genome shotgun sequence.
ACCESSION   DACADA010000132 DACADA010000000
VERSION     DACADA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.134"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..92)
                     /locus_tag="ISB17_003633"
     CDS             complement(<1..92)
                     /locus_tag="ISB17_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1981524.1"
                     /translation="MLRPSRSRRFHRAGEGLPALCGATPFRARA"
     gene            complement(325..645)
                     /locus_tag="ISB17_003634"
     CDS             complement(325..645)
                     /locus_tag="ISB17_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981525.1"
                     /translation="MRMSLMFAAVTLLTGINLFFIPFQPVPALWGAILAVAFVLINVV
                     ADKLRLWSGIAWIIVAGIIITLMLKGKEPGDELRGLITVFAFLASAWYCVVQYRKEQG
                     ESQS"
     gene            complement(680..934)
                     /locus_tag="ISB17_003635"
     CDS             complement(680..934)
                     /locus_tag="ISB17_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1981526.1"
                     /translation="MSKWNIASFSKEEQDKVAVDKVAAAVAWQERMNKPVVPELVERE
                     QPEHLREYFHERLRVHRLNSQQLPRANAPEYNKPGDEQQK"
     gene            complement(1122..1313)
                     /locus_tag="ISB17_003636"
     CDS             complement(1122..1313)
                     /locus_tag="ISB17_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981527.1"
                     /translation="MKLAPQSRDILRQYKALINARRRDAGQRELTTAQVMDEICEYMT
                     CQCAVYIGGHFILQGGKAR"
     gene            complement(1356..1862)
                     /locus_tag="ISB17_003637"
     CDS             complement(1356..1862)
                     /locus_tag="ISB17_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP1981528.1"
                     /translation="MTSSVTVPAVYVGTYHQYNGGSIFGKWFDLTDFDDEDEFYDACR
                     ALHAAEDDPEFMFQDWEGIPSQFASESSVKWAFIEAFRQAQDEGRAAAFVAWADYTGE
                     CDYDAFDEAYCGEAESEEDFAYGFVEDHGLLNEVPESLRVYFDYEAYARDLFSSGYVF
                     HEGYVFSN"
     gene            complement(1905..2570)
                     /locus_tag="ISB17_003638"
     CDS             complement(1905..2570)
                     /locus_tag="ISB17_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
                     /protein_id="HAP1981529.1"
                     /translation="MQGCADAAILARSQVFYDARRSSRRFLAQVSLENRPDQLRAISA
                     LRPPVSPLHLPLFAGLSCRHGTTRCRSLCDYGGLSMQYVNTCPVLNVEESDRLSFMPY
                     LFGTDFMLAELQVYALAKKWMPEYEGGFWHFIRLPEGGGYMMPDCGRVHLVSSENWFD
                     RTVSADAAGIILTSLAINRRLAAHHDSSNPALTRLYMLRDAQLWNHITFHPECSAIYA
                     ALD"
     gene            complement(2585..2941)
                     /locus_tag="ISB17_003639"
     CDS             complement(2585..2941)
                     /locus_tag="ISB17_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981530.1"
                     /translation="MSPCRLRVHPARFPGLLGEHNPLLSPCRVLAVPCLHFSPAAAEA
                     GVRWRRHPRIRQSPACRCRRLRFTRPHPSGPWPYYCVCRVATSASAFLPVVEKRGRET
                     LYSTSCIATGSAVPSL"
     gene            complement(3014..3781)
                     /locus_tag="ISB17_003640"
     CDS             complement(3014..3781)
                     /locus_tag="ISB17_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981531.1"
                     /translation="MMKKETLNTLILRHGDRMLRQAGWPDTVDMAPVAPETVPGWLAA
                     CGSLSGVEILTLTEQLCQPLTYGRAALLLASARRLAGTPARLHLFPVQAYPHPERLAD
                     CQVIRLPYAQEWLTAAECDDLLAFLKASLTQISEIVHRDTKRIAAALTPSVNPRLMDR
                     RIGDWRLLAVEYDHDNCLDEDETDRLDQVLDAILIRDARFCPVLLTLVNEREETIRSA
                     GVIADQLRFTDTPVRRWFDRRVLREMVREARASRTQE"
     gene            complement(3835..4254)
                     /locus_tag="ISB17_003641"
     CDS             complement(3835..4254)
                     /locus_tag="ISB17_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1981532.1"
                     /translation="MYGTREDLCRMLNEQYPAETPLNLIIWTPADIEALADGMEYSFS
                     EHDVRAVLERMDTIPEEQRLESGVSAGLVMALIDQVKENGQRVTVPVDLLETLLITAE
                     QALWDREWTARDRNLPVPESVMRRLADTAKVRALLKS"
     gene            complement(4264..4485)
                     /locus_tag="ISB17_003642"
     CDS             complement(4264..4485)
                     /locus_tag="ISB17_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981533.1"
                     /translation="MNYDGHEALRRDMAGLANNLCDLKTTLKVLEDTYHYRYDGLAER
                     LAGISLRRLSVLMDEAFNIALMLDESFLD"
     gene            complement(4485..5186)
                     /locus_tag="ISB17_003643"
     CDS             complement(4485..5186)
                     /locus_tag="ISB17_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1981534.1"
                     /translation="MSRFILGNCIDVMRGFPDRAVDLIVTDPPYLVGFKDRQGRQIAG
                     DVTDEWLQPATLEMYRVLKKDALMVSFYGWNRVDRFMAAWKAAGFYAVGQLVFTKNYA
                     SNRRNARARRGFVDYCHEGAYVLAKGRPVPPLKPLPDVLPFPYTGNTLHPTQKPVEAL
                     QPLIESFSAPGAIVLDPFAGSGSTCVAAYRAGRRYIGIEMLAQYHRAGTERLAAMHRA
                     VNTPAANDEWLPEAA"
     gene            complement(5388..5504)
                     /locus_tag="ISB17_003644"
     CDS             complement(5388..5504)
                     /locus_tag="ISB17_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981535.1"
                     /translation="MPVIPRSPGVNASLPPAASVSRPVWYGTGGFSAPARRR"
     gene            complement(5623..5853)
                     /locus_tag="ISB17_003645"
     CDS             complement(5623..5853)
                     /locus_tag="ISB17_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981536.1"
                     /translation="MSPGFRNVLRLARIPVDSSEPVRRKFGSRLIGIVTVCLLPLLVF
                     DVPQWVFILSQVIWGAGVYIGLSLAGDKSLNR"
     gene            complement(5917..6588)
                     /locus_tag="ISB17_003646"
     CDS             complement(5917..6588)
                     /locus_tag="ISB17_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mediator of plasmid stability"
                     /protein_id="HAP1981537.1"
                     /translation="MPDGQYSFYLHSDDRTDIAAMATISTISQPLRGEFIRTAATAGA
                     VVYQVDARLPALIPVFFAGQLSAVRLCAVMALVSGTWSSLTGLPDEPENGEAALPVSP
                     ETEYQRRRYTLTLQNGRSGERVESVLTEASSRLRGDLLRNLIITGLALHTTAPELPRL
                     LASMPVPPTSVSELQALVQQMAGTADVNRAAAAVPDTPAASAAPVSGADTREIKKNMR
                     RAFGD"
     gene            complement(6591..7562)
                     /locus_tag="ISB17_003647"
     CDS             complement(6591..7562)
                     /locus_tag="ISB17_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HAP1981538.1"
                     /translation="MNIYCDDGSTNVKLAWFEGDELQTRVSANSFRHGWKVAEFSAAT
                     FNYQVGTLKYTWDSVSRDAIPTTNVEYQYGDLNLLAVHHALLNSGLEPQPVSLTVTLP
                     LSEYYDGDCQRNEENIRRKRENLMRELVLNKGRAFTVTDVKVMPESLPAAFSRLAELK
                     PGPAETTLIIDLGGTTLDAGVIVGQFDDISAVHGNPSVGVSQVTRAAAGALRAADSET
                     SALIADTIIRNRNDRQYLQRVINDAGKIDEVRNKITEAITSLGARVTSELTAFRNVNR
                     VFLVGGGASLIEEAIRQAWPLAPDRIEVIGDPQMALAREIALYNKED"
     gene            7796..8227
                     /gene="umuD"
                     /locus_tag="ISB17_003648"
     CDS             7796..8227
                     /gene="umuD"
                     /locus_tag="ISB17_003648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1981539.1"
                     /translation="MKLQLFSTGKELPPQAHPLFADLASCGFPSPAADYVESDLDLHD
                     YCIRHPSATYYLRASGDSMADGSLYNGDLLVVDSAEKPRHGDIVVASVQGEFTVKRLL
                     LTPRLTLQPMNASWSPIYPDPDELDIFGVVTHIIHRPREMY"
     gene            8227..9498
                     /locus_tag="ISB17_003649"
     CDS             8227..9498
                     /locus_tag="ISB17_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1981540.1"
                     /translation="MFALADVNSFYASCERVFRPDLKGKPIVVLSNNDGNVIARSAEA
                     KPWIKMGTPWFQIKNERYPEKIYAFSSNYELYASMSARVMNCLEELAPRVEQYSIDEM
                     FLDLTGVEHCMGLEDFGRQLRQHVYDCTRLTIGVGAGPTKTLAKSAQWASKEWKQFRG
                     VLALTRGNPQRTRKLLSLQPVEEIWGVGNRIARKLNVLGIKTALDLALTNPAFIRKNF
                     SVVLERTVRELNGESCLSLEEAPPTKQQIVCSRSFGVKITEYESLRQAICQHAERASE
                     KLRKEHQYCRHISVFIKTSPFAIKEPYYGNVATEKLLTPTQDTRDIIAAATMALERIW
                     RDGHRYAKAGVMLNDFTGSGVSQLQLFDERPPRPHSAELMRVLDGINNSGLGKVWFAG
                     RGIAPSWQMKRDMLSPAYTTRWRDIPVARIG"
     gene            9910..10785
                     /locus_tag="ISB17_003650"
     CDS             9910..10785
                     /locus_tag="ISB17_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP1981541.1"
                     /translation="MNENVYIGEVIGPDCESVNNKLTVTSKATVQPVALMRLNVFVPS
                     SRPAKGTRTSIDATSVLKDLEFSRREGYDDIRITGERLSMAIDFKVWVGIIEAFSKYG
                     LKTNSIKLTFHEFAGMCRFESKRFDSRLRKSISEALTRIRGKTITFTRSDSEGLQKVS
                     VTGLLKTGELDFANDQVELEADQRLWELYQTDYTVLLRHKPINALTRKEAAQALYTFI
                     ESLPTNPVPISFKRIRDRLSLLSPVKEQNRIIKLALKQLQDIGYLDHSITKKGKETFI
                     LIHRRSANLKPGTAV"
     gene            11450..12076
                     /locus_tag="ISB17_003651"
     CDS             11450..12076
                     /locus_tag="ISB17_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HAP1981542.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANQAADVLKD
                     YPEMRLASSRVCDRKAHRDAWAESMTIFETQNDKAQQEIESLVKEVIL"
     gene            12073..>12374
                     /locus_tag="ISB17_003652"
     CDS             12073..>12374
                     /locus_tag="ISB17_003652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981543.1"
                     /translation="MRNRANTPSRDEAAFINGGTAGIDNTKVTSERPLAVSKAKPVSI
                     SFFEENLKDIDDLITGEMISGKSRVNRSDVVRAAVEALKSLSKTEISRLIAEAKQR"
BASE COUNT         2987 a         3339 c         3343 g         2705 t
ORIGIN      
        1 aacgcgcggg cgcgaaacgg cgttgccccg cagagtgccg gcagtccttc gccggcacgg
       61 tgaaagcggc gcgaccggga cggtcgcagc aagcgctgcc gttgaccttg acgtcagaca
      121 tcagacacta gccgccaggc ccgaaaccgc gtagcgggtt cggcggagct taccgcgtct
      181 tttcgcggtt agcggagtgt ggctcgacga ctgaaccgcg ccagcgggtc aggagccgaa
      241 ggtggccccg gagcagattg tgtgaaagga aagtgaggca aagccagagg aagagcagca
      301 gcaagatgaa gggtaccggt caggtcagga ctgagattcg ccctgctctt ttctgtactg
      361 tacaacgcag taccaggccg aagccaggaa agcaaaaacg gtgatcaacc cgcgtaattc
      421 gtcacccggc tctttcccct tcagcatcag cgttatgatg atgccggcaa cgataatcca
      481 cgcaatgcca ctccagaggc gcagcttatc cgccacgaca ttaatcagaa caaaagcaac
      541 ggccaggata gccccccaca gagccggaac aggctgaaac ggaataaaaa acaggttaat
      601 accggttaaa agcgtcacgg ctgcaaacat cagagacata cgcataaaat ttcctccctg
      661 gacacactga ctataagaat cacttctgct gctcatctcc gggtttatta tattcgggcg
      721 catttgcgcg cggcagctgc tgactgttca gtcgatgtac gcggagccgt tcatggaaat
      781 attcgcgcag gtgttcaggc tgttcccgtt caacaagttc cggcacgacg ggcttgttca
      841 tgcgctcctg ccaggcaaca gcagcagcca ccttatcgac ggcaacttta tcctgctcct
      901 cctttgaaaa agaggcgata ttccactttg acatatgctt taccggtctg ctttacagga
      961 agatatccct gataatacgc gaattccggg gcaacagcga ctgacaacgg agaggagagt
     1021 gcgcggcgcc ggaggcgcgg cgacgtctct ctccggcagg cgcagcctgc ccgggttttc
     1081 gcgcggccgg aagcggcaga ggttcccccc tgccctgtga tttatcgtgc ctttcccccc
     1141 tgaagaatga aatggccgcc aatatagacg gcgcactgac aggtcatgta ttcgcagatt
     1201 tcatccatca cctgcgcagt ggtcagttcc cgctgaccgg catcgcggcg gcgggcatta
     1261 atcagggctt tgtactgtcg caggatatcg cggctttgtg gtgcaagttt catgtgcttt
     1321 ctccttaacc ggcgggcttc cccgccggat agtcatcagt tgctgaatac atagccttca
     1381 tggaagacat agccgctgct gaacagatcc cgcgcgtaag cctcataatc gaaatagacg
     1441 cgcagggatt caggcacctc gtttaacagg ccgtgatcct ccacaaagcc ataagcaaaa
     1501 tcctcctcgc tttcagcctc gccacaatac gcctcatcaa aggcgtcata gtcgcactcg
     1561 ccggtatagt ccgcccaggc cacaaacgcg gcagcgcgtc cttcgtcctg cgcctgccgg
     1621 aatgcctcga taaacgccca cttaacagag gattcagagg caaactgaga cgggatccct
     1681 tcccagtcct gaaacataaa ctcggggtca tcctcggcgg catgcaacgc ccggcaggcg
     1741 tcataaaatt cgtcttcgtc gtcgaagtcg gttaaatcaa accatttgcc aaaaatactg
     1801 ccgccgttgt actggtgata tgtgcccacg tagacggccg gaacggttac tgatgaagtc
     1861 atttttgtct ccctgcgggg cggtgtaacc gccccggtcg ttaatcagtc cagcgcggcg
     1921 taaatagcac tgcattcggg gtgaaaggtg atgtgattcc agagctgggc atcacgcagc
     1981 atataaaggc gagtcagcgc cgggttactg ctgtcatgat gggccgccag acggcgatta
     2041 atcgccaggc tggtcagaat aatgcccgcc gcgtcggcgc tgacagtgcg atcaaaccag
     2101 ttttcgctac tgaccagatg tacccggcca caatccggca tcatgtagcc gcccccctcc
     2161 ggcaggcgaa taaaatgcca gaagccgccc tcatattcgg gcatccattt tttggccaga
     2221 gcgtacacct gtaattccgc gagcatgaaa tccgtgccga acaggtacgg cataaaagac
     2281 aggcggtcgg actcctcaac attcaggacc gggcatgtat tcacatactg catggataaa
     2341 cctccgtaat cacatagact gcggcaccgg gttgtgccgt gccgacacga cagcccggcg
     2401 aaaagcggaa gatgcagggg cgacaccgga ggccgcagcg cagatatagc gcgaagctgg
     2461 tctggacggt tttcgagcga aacctgagcg agaaaacgac gggaagaacg gcgagcatca
     2521 taaaaaacct gcgaccgggc gaggatggcg gcgtcagcgc acccttgcat ctgcagcggc
     2581 cgggctaaag tgaaggcacg gcactacccg tcgcgatgca ggaggttgaa tacagggttt
     2641 ctctcccccg tttttcgacc accgggagaa aagcggaagc agatgtcgca acgcgacaga
     2701 cgcagtaata aggccacgga ccggagggat ggggccgggt gaagcggagc cggcggcaac
     2761 ggcaagccgg cgactgcctt atccggggat ggcggcgcca gcgaactcca gcctctgcgg
     2821 ctgccgggct aaagtgaagg cacggcacag ccagcacacg gcacggcgat aacagagggt
     2881 tgtgttcacc caaaaggccc ggaaaccggg ccgggtggac gcggagccgg cacggcgaca
     2941 tccttatcgc accacacctc ctgtgccggc aggctgacaa gggagggtcc ccctcccctg
     3001 acgctccggg ctgtcactcc tgagttctgc tggcgcgggc ctctctcacc atttccctta
     3061 aaacccggcg atcaaaccag cgccggaccg gagtgtccgt aaaacgcagc tgatcagcga
     3121 taacccctgc gctcctgata gtttcctccc gctcgttaac cagggtgagc aggaccgggc
     3181 agaatcgcgc atcgcgaatc agaatggcat cgaggacctg atccagcctg tcggtttcgt
     3241 cttcgtccag gcagttatca tgatcatact ccacggccag caagcgccag tcaccgatcc
     3301 gcctgtccat cagacgggga ttgacggacg gcgtaagcgc ggcggcgatg cgcttcgtat
     3361 cccggtggac gatttccgat atctgcgtga gggaggcttt caggaaggcc agcagatcgt
     3421 cacattccgc cgctgtcagc cactcctgag cgtaaggcag gcggataacc tgacagtcag
     3481 ccagccgctc cggatgcgga tatgcctgca ccggaaagag atgcagacga gccggggttc
     3541 ccgccagccg ccgggcgctg gcgagcaaca acgctgcccg gccatacgtc agcggctgac
     3601 aaagctgttc cgtaagcgtg aggatttcca cgccactgag agagccgcag gcggcaagcc
     3661 agccgggtac ggtttcgggg gcgacgggcg ccatatcaac agtgtcgggc cagccagcct
     3721 ggcgcagcat ccggtcgccg tgacgcagaa taagcgtgtt aagggtttct tttttcatca
     3781 ttatcgtctc tgtccgggtt atatcacccc gccgcgtggc ggggcgatct gctgtcagct
     3841 tttcagtaat gcccgcactt ttgctgtatc cgccaggcgg cgcattacgc tttccggcac
     3901 cgggagattg cgatcccgtg cggtccattc cctgtcccac aatgcctgtt cggcggtaat
     3961 cagcagggtt tcaagcaggt caaccggcac agtcaccctc tgcccgttct ctttcacctg
     4021 gtctatcagt gccatcacca ggccggctga cacgcccgac tcaagccgtt gttcttccgg
     4081 tatagtgtcc atgcgttcca gtactgccct tacgtcatgt tctgaaaagg aatattccat
     4141 cccgtcagcc agtgcttcaa tatccgccgg ggtccagata atcaggttca gcggggtttc
     4201 tgcgggatat tgctcattca gcatcctgca aagatcttca cgggttccgt acatgttctc
     4261 tcctcaatcc agaaagcttt catcgagcat cagtgcaata ttgaacgcct catccatcag
     4321 gacgctgaga cgtcgcagac tgataccggc aagccgttcc gccagtccgt cgtaccggta
     4381 gtggtacgtg tcttccagca ctttcagggt ggttttcagg tcgcaaagat tgttcgccag
     4441 cccggccata tcgcggcgca gggcttcgtg tccgtcgtaa ttcattacgc ggcctccgga
     4501 agccattcat cgttcgccgc cggggtattt acggcccggt gcatggcggc cagtcgttcg
     4561 gttccggccc ggtggtactg cgccagcatt tcgatgccga tatagcggcg tccggcacgg
     4621 tatgcagcga cgcaggtcga accgcttccg gcaaacgggt cgagaacaat cgcgcccgga
     4681 gcgctgaatg actcaatcag cggctgtagc gcctccactg gtttctgcgt cggatgcagg
     4741 gtattaccgg tatacgggaa gggcagcaca tccggcagcg gtttaagggg cggcacgggg
     4801 cggcctttgg caaggacata agccccttca tggcagtagt ccacaaatcc gcgtcgggcc
     4861 ctggcgttac gacggttgga ggcatagttt ttggtgaaca caagctggcc cacggcatag
     4921 aatcctgccg ccttccacgc ggccataaag cggtccacac ggttccagcc gtagaagctc
     4981 accatcagcg cgtctttttt aaggacgcgg tacatctcca gcgtggccgg ttgcagccat
     5041 tcgtccgtca catccccggc aatctgtctg ccctgacggt ccttaaaacc gacgaggtaa
     5101 ggcggatcgg tcacaataag gtccacggca cggtcgggga atccgcgcat cacatcaata
     5161 cagttcccga gaatgaatct ggacatggca gcactcctgt agtctgaggg ggcaggaggc
     5221 atcaccgcga taccgttccc tgccgcacgc tgcctgttca tcgcgtccgg tcacgggtca
     5281 ggccgaccgt ggaataccgc agccgtccgt cttttggacg gcaaggatat gccgcgaaag
     5341 cggcttgaag ggtgttcctt aaaagggcga gggactggcg tacactctca acgacgccgg
     5401 gccggagcgg aaaaaccgcc cgtcccgtac cagaccggac gcgagacact ggcagcgggc
     5461 ggcaggcttg cgttaactcc aggcgaacgg ggaattaccg gcatacctgt ccacaaatcc
     5521 ggggcttaag attgtggata acctgaaggc aaacgacgga gaagcgcggg agaacagacg
     5581 ccaccacgcc tgttcttttt ttgaacaacc ggaaagttta tatcagcggt tcagggattt
     5641 atccccggcc agtgacaggc caatatagac gcccgccccc cagatgacct gcgacaaaat
     5701 gaaaacccac tgcggaacgt cgaaaaccag aagcggaagc agacataccg tcacaatgcc
     5761 aatcagccgg ctgccaaatt ttctgcgcac cggttcactg ctgtcgacgg gaatgcgggc
     5821 gagacggaga acatttctga atcctggtga catgagaacc tccctgggga tgtcagtcat
     5881 ttgaaatcgg tatagtagtg gatgtgattc gtactgttag tcaccgaatg cacgccgcat
     5941 atttttcttt atttcccggg tatccgcccc ggacaccggc gcggcagatg ctgccggcgt
     6001 atccggtact gcagctgcag ccctgttcac gtcagcggtg ccggccatct gctgaaccag
     6061 agcctgcagc tcactgaccg aggtgggcgg taccggcata ctggccagca ggcgcggcag
     6121 ttcaggcgcc gtcgtgtgga gggcaagacc ggtgataatc aggtttctca gcagatcccc
     6181 acgcagacgg gatgacgctt ccgtcagcac actttccacc cgttccccac tccggccatt
     6241 ctgaagcgtc agggtgtaac gccggcgctg gtattctgtc tccggtgaca cgggcagagc
     6301 tgcctcacca ttttcgggct catccggaag gccggtgagt gaggaccagg ttccggagac
     6361 cagggccatc acggcgcaaa ggcggacagc gctcagttgc ccggcaaaga acaccgggat
     6421 cagcgccggt aaacgggcat ccacctggta taccaccgcc ccggcggtgg cggcggtacg
     6481 gatgaactca ccgcgcaggg gctgtgagat ggtgctgatg gttgccattg ccgcgatgtc
     6541 ggtgcgatcg tcgctgtgca gatagaaaga atactggccg tccggcacgt ttagtcctct
     6601 ttattgtaaa gggcaatctc cctggccagc gccatctgcg gatcgcctat aacctcgata
     6661 cggtcaggag ccagaggcca ggcctgacgg atagcttctt ctatgaggga agcccctccc
     6721 ccgaccagga atacccggtt aacgttcctg aaggccgtca gctcactggt gacacgcgcc
     6781 cccagggaag tgatcgcctc tgttattttg ttccgaacct cgtcgatttt accggcatcg
     6841 ttgatcacac gctgcagata ctgacgatca ttgcggttgc ggatgatagt gtcagcgatg
     6901 agggcgctgg tttcgctgtc tgccgcacgc agcgcgcctg cagctgcgcg ggtgacctgt
     6961 gacacgccga cagacggatt accgtgcacc gcactgatat catcaaactg gccgacaatc
     7021 acaccggcat cgagcgtggt accaccgaga tcgataatca gggtggtctc agcggggccg
     7081 ggtttcagct ccgcgagacg ggagaacgct gccggtagtg attccggcat caccttcacg
     7141 tccgttacgg taaatgccct gcccttattc agaaccagct cacgcatcag gttttcccgt
     7201 ttgcgtcgta tgttctcctc gttccgctgg cagtccccgt cgtaatactc actcaggggc
     7261 agcgtcacgg tcaggctcac cggctgcggc tccagtccgc tgttcagcag cgcatgatgc
     7321 acggccagca ggttgagatc accgtactga tattccacgt ttgttgtcgg gatggcatcc
     7381 cgactgacgc tgtcccaggt gtatttcagc gtccccacct gatagttgaa cgttgccgca
     7441 ctgaattccg ccactttcca gccatgccgg aaggagttcg cagaaacccg ggtctgaagc
     7501 tcatcgcctt caaaccatgc cagcttaaca ttagttgaac catcatcgca gtaaatattc
     7561 atcaatatgc tccgttagtt aaactcaatt tgataccaat tataagagca aaatgaaaaa
     7621 aacaaagtaa catttctcaa tatgagcaat ataaaaatat caaaatgatt aatataagca
     7681 ttttgaacac ctttatttac tcaaaatgtt cttaaggcat gcgatctgga ttatcgtcac
     7741 gctgttaaat atactgtatg cataaccagt aaaaattaga gtgtcaggag gaaagatgaa
     7801 actacagctt ttcagcaccg ggaaagaact cccgccacag gcccatccat tgtttgccga
     7861 tctggccagc tgcggttttc ccagccctgc tgctgactat gtcgagtccg atctggatct
     7921 ccatgattac tgcatacggc atccgagcgc gacgtactac ctgcgtgcca gcggagacag
     7981 catggccgac ggcagtctgt acaatggcga tctgctggtt gtggacagtg ctgaaaagcc
     8041 gcggcacggc gatatcgttg tcgccagcgt acagggggag tttacggtga aacggcttct
     8101 gctgacgccg cgactgacgc tgcagcccat gaatgcctcc tggtcgccga tttatcctga
     8161 tcccgacgag ctggatatct ttggcgttgt cacgcatatc atccaccgcc cgagggagat
     8221 gtactgatgt ttgccctggc cgatgtgaac agcttctatg ccagctgtga acgtgttttc
     8281 cgcccggatt tgaaaggaaa gccgatagtc gtcctgagca acaacgacgg gaacgtaatc
     8341 gctcgcagtg ccgaggcgaa gccctggatc aaaatgggaa ctccgtggtt tcagataaaa
     8401 aacgaaaggt acccggaaaa aatatatgcg ttttcaagta attatgaact ttacgcctcg
     8461 atgtcggcgc gcgtgatgaa ctgcctggag gaactggccc ccagggtgga acagtattcc
     8521 attgatgaga tgttcctcga cctgaccggc gtggagcact gtatggggct tgaggacttc
     8581 ggccggcagc tgcgtcagca cgtgtatgac tgtacccgcc tgaccattgg cgtgggcgcc
     8641 ggaccaacga aaaccctcgc aaaatcagcc cagtgggcct caaaagagtg gaaacagttt
     8701 cgtggcgtac tcgcgctgac caggggaaat ccgcaaagga cgcggaaact gctttcactg
     8761 cagccggttg aagaaatctg gggggtgggt aaccgtattg cacgcaagct aaatgtgctg
     8821 ggcataaaaa ccgcgctcga tctggctctg acgaacccgg ccttcatccg caaaaatttt
     8881 tctgtggtcc ttgagcgaac ggtacgggaa ctgaacgggg aaagctgcct ttcgcttgag
     8941 gaagcccctc cgacgaaaca gcaaattgtc tgcagccgca gttttggcgt gaagatcacg
     9001 gagtacgagt cactccgtca ggctatctgt cagcacgcag agcgggcctc tgaaaaactg
     9061 cgtaaagagc accagtattg ccggcatatt tccgttttca tcaaaacgtc tccgttcgcc
     9121 attaaagagc cttactacgg caacgtggct acggaaaaac tgcttacgcc cactcaggac
     9181 accagggaca tcattgcagc cgccacaatg gctctggagc gtatctggag ggatgggcac
     9241 cggtatgcca aagccggagt tatgctgaat gacttcaccg gctccggcgt ttcgcagctg
     9301 cagctgtttg acgaacgccc gccacgccct cacagtgctg agctgatgag ggttctggac
     9361 gggatcaaca actcagggct gggtaaagta tggtttgccg gtagaggaat tgcgccatcc
     9421 tggcaaatga aacgcgatat gctctcccct gcgtacacca ctcgctggag agatatcccc
     9481 gttgcgagga tcggttaaat tttgcctttc agagagagcc agcagtgctc gccgcagccg
     9541 aacgacaggg cgaagcccgc gagtgagcga ggaagcacca ggggagctga agccttttaa
     9601 agcaccttac gcacttaagt tctatagatt ccataatgag ttatccacat atcctttgag
     9661 tagatccaat aacttaccct tagttatcca aatagatcca aactgatccc tttagcggca
     9721 aaacctttgt ttacgggctt cgtagcgatt tcaggtgtgt cgtagtggga aaaaggtgtt
     9781 ctgaagcggg aaaaaggtgt gtcaaaacgg ggtaagggtg ggtagaatcg ggaagaaagg
     9841 tgtgtcgaaa cgggaaatag ttaatattta ggcacatatc gatcatatgc tatgaatgga
     9901 tataactata tgaatgagaa tgtttatatc ggcgaggtta taggtccaga ttgtgaaagt
     9961 gttaacaata aactcaccgt cacaagtaag gccacagttc agcctgtagc cctgatgaga
    10021 ttaaatgttt ttgttccttc aagcaggcct gcaaagggca ccagaacatc aatcgatgcg
    10081 acctctgttc tcaaggatct ggaattctca aggcgggagg ggtacgacga tattcgtata
    10141 accggtgaac ggttgagtat ggccatcgac ttcaaggtat gggtcggtat catagaagct
    10201 ttcagtaagt acggtttaaa aaccaacagc attaagctta cttttcatga gtttgctgga
    10261 atgtgccgct ttgagtctaa gagattcgat tcccgtctta gaaaaagtat ttcagaagct
    10321 ttgaccagga tacgtggcaa gaccattacc tttacccgta gcgactcaga gggccttcaa
    10381 aaagtttccg taacaggttt gcttaagaca ggtgaactgg attttgcaaa cgaccaggtg
    10441 gaacttgaag ctgatcagcg actatgggaa ctctaccaaa ctgattacac tgttctgcta
    10501 cgacataaac cgataaacgc tttaacccga aaggaagcag ctcaggccct gtatactttc
    10561 atcgaaagcc tgcctacgaa cccagtcccc atatctttca aacgaatacg agacaggctc
    10621 tcattgctct ctccggtaaa ggaacagaac cgtatcatta aactggccct caagcagttg
    10681 caggatattg ggtaccttga ccacagcatc accaaaaaag gtaaagagac tttcattctg
    10741 atccaccgtc gctcagcaaa tctgaaacca ggaacagctg tataaggtgt gtcaaaacgg
    10801 gaaagcaacg cgattaaggt gtgttgaaac gggaggtctc ttcttaaaca tcttgatagg
    10861 tgtgttgtag ggggaagcct cgccttaaac gtcttaatag gtgtgttgta gcgggatgag
    10921 aacctgttaa atgtgtgttt ttatctgtat atacttacgc accttcccac ttcaacacac
    10981 ctttttggat agaagtgaga agctatcccg cttctcacca ccttttacca aatttgtctc
    11041 accgactccc cacttcgaca caccgaagat tcaaaggctt cccactacgc cccacttttt
    11101 gcttcacgcg gtcataactg gttgatacta ccctccctaa aggtagcctg gagttgaaag
    11161 ccgcattatg tggttctatg gggatttcgg ataaaggcac cactttgcca tcttgttatc
    11221 ttgttatctt gttatcttgc tatcttgcta tcttgctatc ttgctatctt gttatattgt
    11281 tatcttgtta tcctaacatc ttactaattt gttaagatac aaaaacaatt cagttataaa
    11341 tcacacagtt aacaacttaa caagatatta acttaataaa gaaattgcaa tattgtcgcc
    11401 tgtttggcaa tattaagttg ttaacttaac aacttaatat gaggctatga tgataatagt
    11461 attgggtagt caaaagggtg gggttggtaa aagtacactg gcggtatcta tagctgcgta
    11521 tctgatgtca ctgggcaata gagtgatcat tgtcgacgca gatgaccaga aatctgtatt
    11581 gacctggtac aacaatcgtc ctgaagatct tccccatatt ccggttactg gcgcaacagg
    11641 caacatcaaa gccatgctaa aggaacacga gaaatcttat gactttgtca tcgcagactg
    11701 tgctggccgc gacagtgcag aaatgcgtag tgggttgatg gccgctgacg tattcatttc
    11761 tccgttgcgg ccttctcaga tggacctgga tgtagtgcct catacatgct ctgtttttac
    11821 agcagcaaaa gattttaatg aggacgtccg gggatatctc gttttaaaca tgacgcccac
    11881 gaacatgttt gtcaatgaag caaatcaggc agctgacgta ctcaaggatt atcctgaaat
    11941 gcgtctggct agttcccgag tttgtgatcg aaaagcgcat cgagatgcct gggcagaatc
    12001 aatgacaatt tttgaaacgc aaaatgacaa agcacaacaa gaaatcgaat cgttagttaa
    12061 agaggttatt ctgtgagaaa tagagcaaat actccatctc gtgatgaggc agcgtttatt
    12121 aacggtggta ctgctggtat tgataatacc aaagtgactt cagagagacc attggctgta
    12181 agtaaagcaa aaccagtgag catttccttt tttgaagaaa acttaaaaga cattgatgat
    12241 cttataacgg gtgaaatgat ttcaggtaaa agcagagtga atcgttctga cgtagtcagg
    12301 gcagcggtag aggcactaaa aagtttatca aaaacagaga tatccagatt aatcgcagaa
    12361 gcgaaacaac gata
//
LOCUS       DACADA010000133        12190 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.135, whole genome shotgun sequence.
ACCESSION   DACADA010000133 DACADA010000000
VERSION     DACADA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.135"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            237..2630
                     /gene="malP"
                     /locus_tag="ISB17_003653"
     CDS             237..2630
                     /gene="malP"
                     /locus_tag="ISB17_003653"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAP1981544.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWVKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2640..4724
                     /gene="malQ"
                     /locus_tag="ISB17_003654"
     CDS             2640..4724
                     /gene="malQ"
                     /locus_tag="ISB17_003654"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAP1981545.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVIVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4834..6150)
                     /gene="gntT"
                     /locus_tag="ISB17_003655"
     CDS             complement(4834..6150)
                     /gene="gntT"
                     /locus_tag="ISB17_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP1981546.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6514..7089)
                     /gene="nfuA"
                     /locus_tag="ISB17_003656"
     CDS             complement(6514..7089)
                     /gene="nfuA"
                     /locus_tag="ISB17_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAP1981547.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7148..7831)
                     /gene="gntX"
                     /locus_tag="ISB17_003657"
     CDS             complement(7148..7831)
                     /gene="gntX"
                     /locus_tag="ISB17_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAP1981548.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7869..8639
                     /gene="bioH"
                     /locus_tag="ISB17_003658"
     CDS             7869..8639
                     /gene="bioH"
                     /locus_tag="ISB17_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAP1981549.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            complement(8668..9078)
                     /locus_tag="ISB17_003659"
     CDS             complement(8668..9078)
                     /locus_tag="ISB17_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1981550.1"
                     /translation="MRCSNSFYANRRRHASMALLELIQKHIRQRDLIGLVEQMACLLS
                     SGYANDRQIKGLFNYILQTGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSK
                     ALHIAKIMLESGVPLADIMRFTGVSEEELAAASQ"
     gene            complement(9297..9533)
                     /gene="feoC"
                     /locus_tag="ISB17_003660"
     CDS             complement(9297..9533)
                     /gene="feoC"
                     /locus_tag="ISB17_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAP1981551.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            complement(9544..11865)
                     /gene="feoB"
                     /locus_tag="ISB17_003661"
     CDS             complement(9544..11865)
                     /gene="feoB"
                     /locus_tag="ISB17_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAP1981552.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQIASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(11882..12109)
                     /gene="feoA"
                     /locus_tag="ISB17_003662"
     CDS             complement(11882..12109)
                     /gene="feoA"
                     /locus_tag="ISB17_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAP1981553.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
BASE COUNT         3005 a         3099 c         3358 g         2728 t
ORIGIN      
        1 ttgaaaatac gcaacggcca ttttttgcac ttagatacag attttctgcg cggtatggca
       61 ttgatttgct gctaatcctg tggattgcac tagctttaag tggtcgagat cacatttcct
      121 tgctcatccc cgcaactcct ccctgcctaa tcccccgcag gatgaggaag gtcaacatcg
      181 agcctggcaa actagcgata acgttgtgtt gaaaatctaa gaaaagtgga actcctatgt
      241 cacaacctat ttttaacgat aagcaatttc aggaagcgct ttcacgtcag tggcagcgtt
      301 atggcttaaa ttctgcggct gaaatgaccc ctcgtcagtg gtggctggca gtgagcgaag
      361 cactggccga aatgctgcgt gctcagccat ttgccaaacc ggtggcgaat cagcggcatg
      421 ttaactacat ctcaatggag tttttgattg gtcgcctgac gggcaacaat ctgttgaatc
      481 tcggctggta tcaggatgta caggattcgt tgaaggctta tgacatcaac ctgactgacc
      541 tgttggaaga agagatcgac ccggcgctgg ggaacggtgg tctgggacgt ctggcggcgt
      601 gctttctcga ctcaatggca actgtcggtc agtctgcgac gggttacggt ctgaactatc
      661 aatatggttt gttccgccag tcttttgtcg atggcaaaca ggttgaagcg ccggatgact
      721 ggcatcgcgg taactacccg tggttccgcc acaacgaagc actggatgtg caggtaggga
      781 ttggcggtaa agtgacgaaa gacggacgct gggagccgga atttaccatt accggtcaag
      841 cgtgggatct ccccgttgtc ggctatcgta atggcgtggc gcagccgctg cgtctgtggc
      901 aggcgacgca tgcgcatccg tttgatctga ctaaatttaa cgatggtgat ttcctgcgtg
      961 ccgaacagca gggcatcaac gcagaaaaac tgaccaaagt tctctatcca aacgacaacc
     1021 atactgccgg taaaaagctg cgcctgatgc agcaatactt ccagtgtgcc tgctcggtag
     1081 cggatatttt gcgtcgccat catctggcgg ggcgtaaatt gcacgaactg gcggattacg
     1141 aagttattca gctgaacgat acccatccaa caatcgcgat tccagaactg ctgcgcgtgc
     1201 tgatcgatga gcaccagatg agctgggatg acgcctgggc cattaccagc aaaactttcg
     1261 cttacaccaa ccataccctg atgccagaag cgctggaacg ctgggatgtg aaactggtga
     1321 aaggcttact gccgcgccac atgcagatta ttaacgaaat taatactcgc tttaaaacgc
     1381 tagtggagaa aacctggccg ggcgatgaaa aagtgtgggc caaactggcg gtggtgcacg
     1441 acaaacaagt gcatatggcg aacctgtgtg tggttggcgg tttcgcggtg aacggtgttg
     1501 cggcgctgca ctcggatctg gtggtgaaag atctgttccc ggaatatcac cagctatggc
     1561 cgaacaaatt ccataatgtc accaacggta ttaccccacg tcgctgggtc aaacagtgca
     1621 acccggcact ggcggctctg ttggataaat cactgaaaaa agagtgggct aacgatctcg
     1681 atcaactgat caatctggaa aaattcgctg atgatgcgaa attccgtcag caatatcgcg
     1741 agatcaagca ggcgaataaa gtccgtctgg cagagtttgt gaaagttcgt accggtattg
     1801 agatcaatcc acaggcgatt ttcgatattc agatcaaacg tctgcatgag tacaaacgcc
     1861 agcatctgaa tctgctgcat attctggcgt tgtacaaaga aattcgtgaa aacccgcagt
     1921 ctgaccgcgt accgcgcgtc ttcctcttcg gcgcgaaagc agcaccgggc tactacctgg
     1981 cgaaaaatat tatctttgcg atcaacaaag tggctgacgt gatcaacaac gatccgctgg
     2041 ttggcgacaa gttgaaggtg gtgttcttgc cggattattg tgtttcggcg gcggaaaaac
     2101 tgatcccggc ggcggatatc tccgaacaaa tttcgactgc aggtaaagaa gcttccggta
     2161 ccggcaatat gaaactggcg ctcaatggtg cgcttactgt cggtacgctg gatggtgcga
     2221 acgttgaaat cgctgagaaa gtcggcgaag aaaatatctt tatttttggt cataccgtgg
     2281 aacaagtgaa ggcaattctg gccaaaggct acgacccggt gaaatggcgg aagaaagata
     2341 aggtgctgga cgcagtattg aaagagctgg aaagcggtaa atacagcgac ggcgataagc
     2401 atgccttcga ccagatgctg cacagtatcg gcaaacaggg cggcgatccg tatctggtga
     2461 tggcagattt cgccgcttat gtggaggcac aaaagcaggt ggatgtgctg taccgcgacc
     2521 aggaggcctg gactcgcgcg gcgatcctca ataccgcccg ctgcggtatg tttagctcgg
     2581 atcgctctat tcgcgattat caggctcgta tctggcaggc aaaacgctaa ggaagttcga
     2641 tggaaagcaa acgtctggat aatgccgcgc tggcggcggg gattagcccc aattacatca
     2701 atgcccacgg taaaccgcag tcgattagcg ccgaaaccaa acggcgtttg cttgacgcga
     2761 tgcatcaacg taccgccacg aaagtggcgg taacgccagt cccgaatgtc atagtttata
     2821 ccagcggcaa aaaaatgccg atggtggtgg agggcagcgg cgaatatagc tggctgctga
     2881 ccaccgaaga aggaaagcag tacaaaggcc atgtgacggg gggcaaagcg ttcaatctac
     2941 cgacgaagct gccggaaggt tatcacacgc tgacgcttac ccaggacgac cagcgcgcgc
     3001 attgccgggt gattgtcgcc ccgaaacgct gttacgaacc gcaggcgttg ctgaataaac
     3061 aaaagctgtg gggtgcctgc gttcagcttt atacgctgcg atcggaaaaa aactggggta
     3121 ttggggattt tggcgatctc aaagcgatgc tggtggatgt ggcaaaacgt ggcgggtcgt
     3181 ttattggtct gaacccgatt catgcgctct atctggcaaa tccggaaagt gccagcccgt
     3241 acagcccgtc ttctcgccgt tggctgaatg tgatttatat cgacgttaac gcagttgaag
     3301 atttccatct tagcgaagag gctcaggcct ggtggcagtt gccgaccacg caacagacgc
     3361 tgcaacaggc gcgggatgcc gactgggttg attactccac ggttaccgcc ctaaaaatga
     3421 cagcattacg aatggcgtgg aaaggtttcg cgcaacgtga tgatgagcag atgaccgcgt
     3481 ttcgccagtt tgttgcagag cagggcgaca gcctgttctg gcaggcagcc tttgatgcgc
     3541 tacatgccca gcaagtgaaa gaggacgaaa tgcgctgggg ctggccagca tggccagaga
     3601 tgtatcagaa cgtggattcg ccagaagtgc gccagttctg cgaagaacat cgtgatgacg
     3661 tcgattttta tctctggttg cagtggctgg cttacagcca gtttgccgcc tgctgggaga
     3721 taagccaggg ctatgaaatg ccgattggct tgtatcgtga tctggccgtt ggtgtagcag
     3781 aaggtggggc agaaacctgg tgcgaccgtg aactgtattg cctgaaagcg tcggttggcg
     3841 cgccgccgga tatcctcggc ccgttggggc agaactgggg attgccgcca atggacccgc
     3901 atatcatcac cgcgcgtgcc tatgaaccgt ttatcgagct gttgcgtgcc aatatgcaaa
     3961 actgcggcgc attacgaatt gatcacgtga tgtcgatgct gcgtttgtgg tggataccgt
     4021 atggcgagac ggcagatcag ggcgcgtatg ttcactatcc ggtggatgat ctgctctcga
     4081 ttctggcact cgaaagtaaa cgtcatcgct gtatggtgat tggtgaagat ctcggtaccg
     4141 taccggtaga gattgtcggt aagctgcgca gcagcggtgt gtactcctac aaagtgctct
     4201 atttcgaaaa cgaccacgag aagacattcc gcgcaccgaa agcgtaccca gagcagtcga
     4261 tggcggttgc ggcgacacac gacctgccta cgctacgcgg ctactgggag agtggggatt
     4321 taacgctggg caaaacgctg gggctgtatc cggatgaagt ggtactgcgc ggtctgtatc
     4381 aggatcgcga actggcgaag caagggctgc tggatgctct gcataaatat ggttgcctgc
     4441 caaaacgtgc cgggcataag gcatcgttga tgtcgatgac accgacgctg aatcgtggct
     4501 tacagcgcta catcgccgat agcaacagcg ccctgttagg gctccagccg gaagactggc
     4561 tggatatggc agatccggta aatattcccg gcaccagtta ccagtataaa aactggcgac
     4621 gtaaactttc cgcaacgctt gagtcgatgt ttgccgatga tggtgtgaac aagttgctga
     4681 aggatttgga cagacggcgc agagcggcag cgaagaagaa gtagagtgcg gttgatgccg
     4741 gatgcgacgc ttgacgcgtc ttaccggcct actaattaat gccgaatcgt aggccggata
     4801 aggcgttcac gccgcatccg gccatcagta ttatcaaatc accatattca gcagcagaca
     4861 gcctaccaga ccgcacaccg agataatggt ttccagcatc gaccaggatt tgatggtctc
     4921 gccgatagtc aggttaaagt actctttgaa cagccagaag cccggatcgt tcacgtgaga
     4981 gaaaatcaca ctaccggaac caaccgcaat aaccatcagc tccgggctaa cacccgtcgt
     5041 tgcaatcagc ggtgccgcga taccacccgc agtgattgcc gcgacggttg cggaacccag
     5101 cgcgatacgc agtacggcag caatcgacca ggccatcagc agcggagaaa tgttggtttc
     5161 gtgcatcatg gaagcaatgt atttgtccac gccgctgtct accagcacct gcttgaacgc
     5221 accgccacca ccgataatca acagcatcat cgcaatgatt ttgatggaag aaaccagcgt
     5281 gtcgttaatc tgatccattg aacggccacg gttcagacca aaggtgaaca tcgcgatcag
     5341 cacggcaatc agcgtagcca ttaccgggtc accgaggaac tccgctaccg gcaggaaagc
     5401 gtgacctttc ggcaggatca tttcggcaat cgcacgcatc gccatcagaa ccaccggcac
     5461 cagagaggtc cagacgctga cgccaaagct cggcatctct tcttcgctga aggttttcgc
     5521 gctgtacaga ccttccggga tcggcttatc gatacctttc aacacgcgag cgtaaaccgg
     5581 accggcgaga atcacggtcg ggattgccag aatagtaccg tacagcagag ttttacccat
     5641 atcggcattg aaaatggtgg caatcgcagt cggacccgga tgcggtggca ggaagccgtg
     5701 ggtcacagac agtgcagccg ccattggtac accaacatac agcagtggaa tattcgcgga
     5761 agccgcgatg gtaaacacca gcggcagcat cagcacgaaa cccacttcat agaacagggc
     5821 aaaaccaacg gtaaaaccgg tcagtaccac cgcccactgg atgtgttttt taccaaattt
     5881 ggcaatcagc gtggtagcga tacgttgtgc accaccgcag tctgccagca ttttgcccag
     5941 catcgcacca aaacccatga tcagggcaag gctaccgagc gttccgccga caccagcttt
     6001 gatggagcca ataactttat ccagcggcat cccttgcatt aatccaacag caagcgccac
     6061 gaggacgaga gcgatgaagc cgttcatttt gaagcggatc atcaggagta ataacaagat
     6121 tacaccgata gcaacaatga ctaatggcat gatttacctg gcctttaatt tgttatgggt
     6181 aacgtcaggt ttctgacgga aaatcctggt tgtcccaact gggaacagag atgttacggc
     6241 accacggctg atgaccccta aaacgagtta gtgtagtcag atgttatgag tgtgtttggt
     6301 gcccatgaga atgatacggg taacattacg tgcttgagaa tcaccagagc ggggtaaatt
     6361 taaattatga gaggttggtc atattatcgt ggggaaacga agagattgga tttgctgaga
     6421 taattctgcg ctaacgtcag actctcgtgc aacgtacagg cccgataaga cgctttagcg
     6481 tcacatcggg caatcgacaa aaggggagag aacttagtag taagagtgct cgccgcgctg
     6541 gtgttcggtg agatcgcgta cacctttcag ctccgggaat tcgttcagca actgcttctc
     6601 gatcccctct ttcagggtga cgtcgaccat ggaacaaccg ttacagccac cgccaaattg
     6661 caaaatggcg taaccgtctt cggtgatttc catcagcgaa acgcgaccac cgtgaccagc
     6721 aagctgtggg ttgatctgtg attgcagcat atactccacg cgctccatca gcggcgcatc
     6781 gtcagccact ttacgcattt tggcgttagg cgctttcagc gtcagctgcg aacccaactg
     6841 gtcggtaaca aaatcgatct ctgcatcttc caggtaagga gcgcttaact catcaacata
     6901 cgcggtcagc aggtcaaatt tcagggcagt gtcggtggct tccacagcgt ccggcggaca
     6961 ataagaaacg ccacattcag cgttaggcgt gccagggtta atcacaaata cgcggatttg
     7021 tgtcccttct tcctggtttg ccagcagttt ggcaaagtgc gcttgtgcag catcggaaat
     7081 acggatcata gtaatggcct aatagttgac tattttagtt ggttataata cgcccatcat
     7141 cgaggctcta caaggttcga caaaggcacc agacctggac agtcgccgca ccattgcgta
     7201 aaagcaactg cgcaatctct gcgacggtac ttccggtggt aacgacatca tccacaatca
     7261 ccatatggcg accttgcaca ggcaattcaa ggcgaaaggc atttttcagg ttgtgtttgc
     7321 gtagccgggc gttaagaaaa tgctgggtcg cggtggcccg tgttcgtgtg acggcttcgc
     7381 tatcccattg gcagtgtagc cagcgtgata acggctgaca cagcaaatcg ctctgattaa
     7441 atccccgacg ccagtgacgg cgctgccata acggaacgct gatgatgcga tcaggcagtt
     7501 gcaagccggt ggtgcgacga gcgtgtaaga cttccaatag taacagacgt gacagggcgc
     7561 tggcgatttc actgcgccgg gaaaacttaa gctggtgaat gagcggactt aatggcggcg
     7621 catagtcggc gaccgtgagc agtctttgcc agggcagcgg tttttgcagg cagcgaccgc
     7681 agggaagatg agagtgtgtg gcgggtaatc cacattgtgg gcataacgtt ttatctgtac
     7741 gggcggcgcg agagcagaca gaacaaatcc cccaatgacc taacgccagc ggcattcggc
     7801 atagccagca taatcccggt actgttagca tatgttcatc cttgtaagta aaaagagaac
     7861 aatagcggat gaataacatc tggtggcaga ccaaaggtca ggggaatgtt catcttgtgc
     7921 tgctgcacgg atggggactg aatgccgaag tgtggcgttg cattgacgag gaacttagct
     7981 cgcattttac gctgcacctt gttgacctgc ccggcttcgg gcgtagccgg ggatttggtg
     8041 cgctatcact tgctgatatg gccgaagtcg tgctgcgaca ggcaccagat aaagccattt
     8101 ggttaggctg gagtctgggc gggctggtgg caagccagat tgcgttaacc catcccgagc
     8161 gtgttcaggc gctggtcacc gtggcgtcgt caccttgttt tagtgctcgc gacgagtggc
     8221 cggggataaa accggacgtg ctggcgggat ttcagcagca actcagtgat gattttcagc
     8281 gtacagtgga gcggttcctg gcgttacaaa ccatgggtac tgaaacggcg cgccaggatg
     8341 cgcgggcgtt gaagaaaacc gttctggcgt taccgatgcc ggaggttgac gtactcaatg
     8401 gcggactgga aatcctgaaa acggtcgatc tccgtcagcc gctgcaaaac gtgcccatgc
     8461 cgtttttgcg attgtatggc tatctcgacg gtctggtgcc gcgcaaagtg gtgccgatgc
     8521 tggataaact ttggcctcac agcgaatcat atatcttcgc caaagcggcc catgcgccgt
     8581 ttatttcaca tccggacgag ttttgtcacc tgctggtggc gttgaagcag agggtgtagg
     8641 tggctttcga aatggcggga cagaacttta ctggctcgcc gcagccaact cttcttctga
     8701 caccccggta aagcgcatga tgtctgcaag agggactccg gattcaagca ttatttttgc
     8761 tatatgcaag gctttggatt gttccccttc ctgccgcaat ctttccgcaa tagtcattaa
     8821 actctccttg tgttttggtg aacgttcggc cacaccatcg ataaaatcgt taaaacgtac
     8881 cgcgtcgccg gtttgcagta tgtaattaaa cagccctttg atttgtctgt catttgcgta
     8941 tccactactt aataagcagg ccatttgctc taccagtcct atcagatcgc gttgacgaat
     9001 atgtttttga attaactcca gcagcgccat gctcgcgtgc cgtctccggt tggcgtaaaa
     9061 agagtttgaa cagcgcatcg tgtggggtgg aactctgctt tttgctcatg gcgttccttc
     9121 acctgttatt tgaatgccgc gactctacgc gccactccac acctgtgcat tagcgagttt
     9181 tcatcccagc gagacgcttt cagaggaact tacgtcgcgc tgctatggaa agccgcacgc
     9241 aaaaaagccc ggatgcaaac gcataccggg ctttcaaaac aggcagtgaa acaaggttaa
     9301 cgcaacgccc accattcgtg cagacaggct tttccttccg ggcagctttt acaactgccg
     9361 gaaagacagc cgtcaggttc ttcctgaatc cgcactgctt ttcccatact ttctagctgt
     9421 ttcagcatgg cgttaatcat tggctgcgga gtgttcagtg tctggctaat ctgggccgct
     9481 tccatacggc cccgtaatgc cagtaaatca cgcacttgaa tgagcgaagc cattgtcgtg
     9541 tccttagtgg caatcgccag tggtactggc cgcacagcaa ctgcttaccg atttgcgcgt
     9601 tgccagcagt tcgatatcca cccggctacg cgcgcgacgc agcaaaccga taaccacgat
     9661 gttaaacaga ataaccgcca gaatgcatac caggctgtag gttggatgct ggctgtagct
     9721 ggcgatctga tagaacaatg ttgccagcga gtaagcgata ttcagccccc acagaatgga
     9781 gaagcccatc cagccacggc ttgattcacg ggcgatggct cccatcaccg agatacatgg
     9841 tacatacagc aggacgaaaa tcaggtagct gtatgcggca gctgcgctac cgaatttctg
     9901 atccatcacg cccatcgctc cggtacccat ttcgccgtcg cctttgcttg cttcaatggg
     9961 gttcatcagt acgctaaggc tgaaggtgtc tttcaggctc tgccaggttt catctaccgc
    10021 actgaacagc tcttcaccga ggttaaattc cgccggattg aactcttcgt cctgaatatt
    10081 ttctgcggtg tagagggtgt tgagcgtacc caccaccact tctttcgcca tggcacctgt
    10141 aaacaggcca accgttgcct gccagttatc ttcatgtacg ccaatcggct tgaagaccgg
    10201 ggtgatcacc cggctgacgg aagccagcgc cgagtcgttg atgttatcga cgattttccc
    10261 gctcaatgag aagctgttga aggcgctcag gaaaatgctg acgatgataa tcactttacc
    10321 agcacgcaga acgaagcctt tcagacgctg ccaggtctgg ataatcaggc ttttaacgtg
    10381 gggtacatgg tagaccggca gctccatgac aaacggcgtc gcttcaccgc gcatgatggt
    10441 gtacttgagc atcaggccag tcagcaccgc cattacgata cccagcatat acagcgagaa
    10501 gaccgccagc gcaccgttct gcccgaagaa ggcagccgcg aatactgcga agatagccag
    10561 acgcgcgccg caggacataa acggtgccat catgatggtc atcagacgtt cacgcggtgc
    10621 atcaagcgta cgtgcgccca ttaccgacgg tacgttacaa ccgaaaccaa cgatcagcgg
    10681 tacaaaggat ttccccggca agcccagcgc ctgcatcaga cggtccatca caaacgccgc
    10741 acgcgccata tagccggagt cctcaaggaa ggagaggaac aggtacatca tgccgatctg
    10801 cggcaccagt ggcagcacgg tgttaatacc gccgcccagg ccttgggcga ggaagatagt
    10861 cagccagtcc gggaagtgga gcgtgtagcc aatccactga ataccatgca caaagagtgc
    10921 tacggagccg acgtcaaaca gcggctgtaa cgccccgccg atgttgatag ccagcaagaa
    10981 catcaggtac atcacaaaga ggaaaatcgg cagaccgagg aaacggttga gcacgatttt
    11041 atctaccgca gtggtgaaac ggctgggttc tgccgtcagg gtgttgctta ccacatcaca
    11101 gatggcagca atgcactggt aacgcgcatc ggcaatgtgc agcgccggat cgtccatctc
    11161 attacgcaga cgggcgaggg cggcatccag atgctgcgac gcttcaccgg cgtaggcgcg
    11221 gctgtagata tcgccttcca gcatttgcag gcccagccag cgacgttgtt tcagcgggat
    11281 gtcggaaggc atcacttttg ccagtgaatc tgcttcgttg agcagcggct gagcgtaatg
    11341 caccagttcc acattctcgt tagctttata gcgatcaatc gccagcttga gcgcttcaat
    11401 accgcgacca cgggttgaaa ccagcgggat caccgggcag ccaagacgcg ccgacagagc
    11461 atcaatttca atacgaatat tttgcttctc ggcaatgtcg agcatgttca gcgccacgat
    11521 gcagggaatg ccgagttcca acagttgcag cgtcaggtac aggttacgct caaggttaga
    11581 cgcatccacc acgttaatca gcaggtctgc gtcgccactc aaaatgtagt gacaggcgat
    11641 ttgctcatcg agcgaggtct gcgatgagat ggtggtcaga gaataggtgc cgggcaggtc
    11701 caccagcgtg acctgatgat cggtggtgga gaattgccct tctttacgtt cgacggtaac
    11761 gccagcccag ttacctacac gctgacgtga gccagtaagc tggttaaata acgttgtctt
    11821 gccagaattt ggattaccaa ttaagccaat ggttaatttt ttcattgttg ttatcaccgt
    11881 attaacagga aaccgcttcc acttctaata aggccagatc ttttttgcgt aataccaggc
    11941 tcacacgacg ggtttcgata tgaatggggt cgccgagtgg agcgacgcgc accacattaa
    12001 aagaggagcc aggtaacatg ccaagagaaa gcagtttttg gcgatatgcc gggctgattt
    12061 cacgggaaaa gccagtaatt ttccacgcag tatctggagt gtattgcata ggtgcctact
    12121 tgtttctcat taactggata actacctcat ggggcgtttg acagcgacat gagaccagat
    12181 gatttgccaa
//
LOCUS       DACADA010000134        12103 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.136, whole genome shotgun sequence.
ACCESSION   DACADA010000134 DACADA010000000
VERSION     DACADA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.136"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(23..661)
                     /locus_tag="ISB17_003663"
     CDS             complement(23..661)
                     /locus_tag="ISB17_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1981554.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(658..732)
                     /locus_tag="ISB17_003664"
     CDS             complement(658..732)
                     /locus_tag="ISB17_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981555.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            complement(732..1652)
                     /locus_tag="ISB17_003665"
     CDS             complement(732..1652)
                     /locus_tag="ISB17_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1981556.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            1790..2689
                     /locus_tag="ISB17_003666"
     CDS             1790..2689
                     /locus_tag="ISB17_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1981557.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3026..4639
                     /locus_tag="ISB17_003667"
     CDS             3026..4639
                     /locus_tag="ISB17_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1981558.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4690..5721)
                     /gene="ynaI"
                     /locus_tag="ISB17_003668"
     CDS             complement(4690..5721)
                     /gene="ynaI"
                     /locus_tag="ISB17_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAP1981559.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            5965..6222
                     /locus_tag="ISB17_003669"
     CDS             5965..6222
                     /locus_tag="ISB17_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HAP1981560.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(6272..7222)
                     /gene="uspE"
                     /locus_tag="ISB17_003670"
     CDS             complement(6272..7222)
                     /gene="uspE"
                     /locus_tag="ISB17_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAP1981561.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(7374..8126)
                     /locus_tag="ISB17_003671"
     CDS             complement(7374..8126)
                     /locus_tag="ISB17_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAP1981562.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(8321..8836)
                     /gene="ogt"
                     /locus_tag="ISB17_003672"
     CDS             complement(8321..8836)
                     /gene="ogt"
                     /locus_tag="ISB17_003672"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP1981563.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(8847..8990)
                     /locus_tag="ISB17_003673"
                     /pseudo
     CDS             complement(8847..8990)
                     /locus_tag="ISB17_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9379..10194)
                     /locus_tag="ISB17_003674"
     CDS             complement(9379..10194)
                     /locus_tag="ISB17_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1981564.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            10291..11319
                     /locus_tag="ISB17_003675"
     CDS             10291..11319
                     /locus_tag="ISB17_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1981565.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVTSKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            11316..>12103
                     /locus_tag="ISB17_003676"
     CDS             11316..>12103
                     /locus_tag="ISB17_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP1981566.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTIFLTSVLALVSFILFAARTLTISNPIMDLHAFKD"
BASE COUNT         3225 a         2699 c         2843 g         3336 t
ORIGIN      
        1 cgatggtagc tatataattg atttacatga agaagggctt atcaccatca gtcccgggtt
       61 gattgatgcc tatattctca ccaatgtgtt tctcaggttt atcgatgata tctgtgatta
      121 atgctgcgac actcttacgt gatacgatcg tacctttgaa tggttcattg cgtgaggtaa
      181 gctcataatc aataatatct tcatcagtca gccaggcagg gcgaagaatt gtatattcca
      241 gtccagaagc ctcaattgca tccgcagccc tgcggaatgg ttttaggggt tcaccaataa
      301 ctgcattatt ccattcgcca aattttcctg gaacttcatc ataaataccg agggaaagaa
      361 caaagatcag acgtttaaca tcacaggctt tcatcgcagc gatcactgaa tttgcctgaa
      421 tatctaaatc ttcccccgta aggttggcat acacaacatc ctgtccctgc atggcctgct
      481 tcagtgctgc atgattcagt acatccccca taataatctt gctattagtt ggatagggtt
      541 tgtggatttt tgctggctga cgggcaaata aagtttgttt aatggtttgt ttatctgcca
      601 gttgattaat cacatggcgg gctatctgac cgccagcacc aagaatcagt acatttttca
      661 tgaaactttt cccagattat tggcagcaat atgccaggcc atatcactat tacggatttg
      721 aatatgtttc attacagagt ttcctcaaag aacggagcta atactgaaat tacttcagcc
      781 acatagactt tgccatcata aagatccata tggtttgcgc cttcaacaat gtgatagcgt
      841 ttatcctggc ttgatgctcg atcgtacagg tcgtcactca tccatttgct cccagccagg
      901 ctgcccgcca caatctgtat cggctgagtc aggtacactt ccgccatatg gtaagcatca
      961 taggtaataa tctggttaag gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc
     1021 gcgcgagggg tgtggtaata ttcccaggcc tgacgcagtt cttcgttcgg cgcatcggac
     1081 tccttcattg gtgccagtgg catagtggcg tattctccgc tgctaatatc gctggttcgg
     1141 gcgtttgaac ccgcgtcaac gtatggtagg gcatcaatag atttcacatt gttttcccaa
     1201 ccattacgga acatcgaacc aatattgacc gcactaacag taccgatggc cttgatgcgg
     1261 cgatcctgaa ttgcagcatt ggctgtatat cctgcaccgg cacaaattcc catcgcacca
     1321 attcgggtat tgtcgacata agaaagcgtt gtcaggtaat caatcacggc actgacgtct
     1381 tcagtacgaa tgtatgggtt ttctaactga cgcggctcgc cgccgctttc accttgataa
     1441 gatgcgtcat aagcaatagt gacaaatcct ttttccgcca gtttttcggc ataggttccg
     1501 gccgtttgct ctttaacgcc cccacctggg tgagatacca caattgccgg atactgacgg
     1561 gtttcatcaa attttggagg gaaatagatc actgcagaca aagagatggt tggattattg
     1621 ctgttagtga agctgacttt attattcatc attccgttcc tcatggagtt gtcggttcgt
     1681 tttaacggtt ggtgatatca ctatagatat tgatcattaa gttgattaga cccaaaatca
     1741 tgattagacc tatcatttaa atgattaata gattgtatgg agatactgaa tgaagcgtga
     1801 agaaatcgct gatctgatgg cgtttgtcgt tgttgcagag gagcgtagct tcactcgcgc
     1861 agcagcccgc ctgagcatgg cgcagtcagc tttaagccag atagtgcgcc gtatagaaga
     1921 acgattggga ttgcggcttc tgacgcgaac cacgcgcagc gttgtaccca ctgaagcggg
     1981 cgagcatctt ttgtctgttc ttggccctat gttgcatgac atagattctg ccctgacatc
     2041 cctgagcgat ctgcagaacc gcccatccgg gacaatacgt attactactg tagaacatgc
     2101 agcaaaaacg atattgttac cagccatgcg cacattcctg aaatcgcatc ctgaaattga
     2161 tattcagctt accattgatt atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg
     2221 cgtccgtctg ggtggggaga tggataaaga tatgatcgcc attcgaatcg ggccagacat
     2281 accaatggct attgttggct caccggatta tttttctcgt cgaagtgctc caacgtcagt
     2341 gtcacaatta atagatcatc aggcaattaa tttgtatctt cccacatcgg gtacagcaaa
     2401 tcgctggaga ttaatacgcg gtggacgtga agttcgtgtt cgcatggaag gtcagctttt
     2461 actgaatacg atagatctga tcattgatgc tgcaattgat gggcatggat tggcgtatct
     2521 accttatgat caggtggagc gggctattaa agaaaaaaaa ctgatacgtg ttttggataa
     2581 attcacacca gatttacccg gttatcacct gtactatcca caccgtcgac atgctggctc
     2641 ggcattctca ttatttatag ataggctgaa gtataaaggt gctgtttagc actacttgct
     2701 gatacattaa tttaattctt ctcttaacgt attctcagtt cctttcaacg ttttggttat
     2761 tttttattct tcgcacaatg gcgacagatg ctgattatga taccgaaaac gggtttgaat
     2821 gtgcgaagcc cgaacgtagt gttcggagtt ctatgtgctt taccgcattt tggagactat
     2881 tatttacact aaatctgatt tgatatattg atacttaaaa catttgatgc ttcctttgtc
     2941 acttttttga tggaagttgt ttgcatttct ttaaggcgaa acaaataatt acgcatcaat
     3001 tttaatgtcg gttagaggga aacttatgaa gcactctgtt tcagtcacgt gttgtgcgct
     3061 gttggtcagc agcatttctc tttcgtatgc tgctgaagtt ccgagcggca cagtactggc
     3121 agagaagcag gagctggtgc gccacattaa agatgagcca gcgtcgctgg atcccgctaa
     3181 agccgtgggc ctgccggaga ttcaggtcat tcgcgatctg tttgaaggac tggtgaatca
     3241 gaacgagaaa ggggagattg tccccggcgt tgcgactcag tggaaaagta atgacaaccg
     3301 tatctggact tttaccctgc gcgataacgc aaaatgggcg gatggcacac cggtaacggc
     3361 gcaagatttt gtctacagct ggcaacgtct ggtggacccg aaaacgcttt cgccatttgc
     3421 atggtttgcc gcgctggcgg gaatcaacaa cgcacaggcg attattgatg gtaaagccac
     3481 gcctgaccag cttggcgtta ccgcagttga tgcccatact ttgaaaatcc agcttgataa
     3541 accgttgccg tggtttgtga atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc
     3601 caacgtagaa agcagtaaag agtggacgaa acccggaaat ctgatcggca atggcgctta
     3661 tgttcttaaa gagcgcgtag tcaatgaaaa actggtcgta gtgccgaata cccattattg
     3721 ggataacgcc aaaacggtac tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc
     3781 agccactaag cgttatctcg cgggggatat tgatatcacc gaatccttcc cgaaaaatat
     3841 gtatcagaag ctgttgaagg atattccggg gcaggtttat acgccgccgc agctcgggac
     3901 ctattattat gcgtttaaca cgcaaaaagg gccgacggca gatcagcgcg ttcgcctggc
     3961 attaagtatg acgatagatc gccgcctgat gaccgaaaaa gtgttgggga cgggcgaaaa
     4021 gccagcgtgg cattttacac cagatgttac cgcgggattt acgccggaac cttcgccgtt
     4081 tgaacaaatg agtcaggaag aactgaatgc gcaggcaaaa actttgttga gcgccgctgg
     4141 ttatggtccg caaaaaccgc tgaagctgac gcttttgtat aacacttcag aaaaccatca
     4201 aaaaattgcg attgctgtag catcgatgtg gaaaaagaac ctgggcgtag atgttaaatt
     4261 gcaaaaccag gaatggaaaa cctatatcga tagccgtaat accggcaatt ttgatgttat
     4321 ccgcgcctcg tgggtagggg attataatga accctccact ttcctgacat tattaacgtc
     4381 aacgcattcc gggaatattt cacgctttaa caatccggca tatgacaaag ttctggccca
     4441 ggcatcgacg gaaaataccg ttaaagcgcg taatgccgat tacaacgcgg cagaaaaaat
     4501 cctcatggag caagcaccga ttgcaccaat ttatcaatat accaatggac gattaatcaa
     4561 gccgtggctg aaaggttatc ccattaacaa ccctgaagat gtggcgtaca gtcggactat
     4621 gtatattgtg aagcattgat gtgatgagaa ctggcgttac ccttgtggat aacgccagtg
     4681 atgtctgatt tagcgaccct ggtccgccgg tgtaatgtta tccatataca gcgtctggct
     4741 ggggaaggca aagtccgcgc cgtgtgactg tacaatatcg ataatcttca aataaacgtc
     4801 ttgctgtgca gcaagccatt cagcccatac cgtggttttg gtaaagcaat aaaccataat
     4861 atttaatgac gaatcagcaa actggttgaa ataaaccagt aaggtttgtc gctggtcgat
     4921 ggccgggtga tttttcagca tctcacgtac ggcttcgaca ataacgccca cttttgccgc
     4981 atcctcataa cgtaaaccaa tggtcgtggt aatgcgccgg ttggtcattc gtccaggatt
     5041 ttctacgctg atcgacgaaa acagcgagtt cggtacgtac aatggacgat tatcaaaggt
     5101 cttaattttg gtaattcgcc agccaatttc cgctactgta ccttcgatat ttctgtccgg
     5161 tgaacggatc cagtcgccaa tactgaaagg acggtcgaaa tagagcataa tcccggaaaa
     5221 gaagttactc agaatatctt taccggccat accgacagcc agaccaccaa taccaccaaa
     5281 ggtcaacaag ccagaaaggc tcatgccgaa atgttcgcca taaagcagaa caagcaccac
     5341 aataatggtg attttgataa tgcgcgacat gatccgcgca ctggtgatat cgcgaccttt
     5401 tttaatctgc tgtttttcaa actgattaat cagcagaaat agcttaatcg tcagaataac
     5461 cgcaatcagg gacgtacaga taaaatcgat aacgcctggg gtgataaatt tgagtttata
     5521 gttttctata acatagttaa tgatgctacc aacagcacta ataattatgg tgtagattaa
     5581 aaattgcacc gcatggaata aaaatccttt tcttttacga tttccacggc gaaaccaaaa
     5641 gctcatcaga atcaatgctg cgcagctacc gaaaataatg accagattaa gcgcattatt
     5701 tgtaaacagt tcagcgatca ttgttttatc aggctcctcc agataattgt cgtcatgccg
     5761 gaaacccctg gcgggactat tttaccgcga caattcattc agatcatcaa tagtcaggga
     5821 aggaagtagc aacattagct aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg
     5881 aggttttcag agcagacaac ggtgaaatgt catggtattg ttacgtttag gtaacaaaaa
     5941 atttgtctgc acaaggatta catcatgatt atggcgaaac tgaagtcagc gaaagggaag
     6001 aaatttctct ttggtttgtt ggcggttttc attattgcgg cgtcggttgt gactcgcgcg
     6061 accatcggcg gcgttataga acagtacaat attccgctgt ctgagtggac gacatcaatg
     6121 tatgtgattc agtcatcgat gatttttgtt tatagcctgg tctttactgt gttgctggca
     6181 atcccgttgg gaatttattt ccttggcggc gaagagcagt aagtaaaaaa taggcccgat
     6241 aactcgggcc ttgtcagtta ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa
     6301 cgggtgtctg atactggtca ggtttaatga ccagcaggtc gcagcgaaga tgatcaatca
     6361 cctgttccgc cgtgttgccg aggaatgctg ctgaaatacc ggtgcgtccg accgtgccca
     6421 gaaccacaat ccccgcctgt aagtgctccg ccaaatcagg aatcacctct tctggcagac
     6481 ctttttctac gtgcgtcatg ttttcattaa tgccgaattt ctgtcgcaga gctttcattg
     6541 ccagcaaatg ttgcccacga atggcatcgt tataaacgct cgggtcaaat tccggcagtt
     6601 caatcgcgat attaattggc gttaccggat aagcgccaac cagatgaact tcggtatggt
     6661 tgacttgttc tgccagttcg atcgtctctt tgaccagttt ttcattgagc gcattatggt
     6721 acggctcttc actggcgaga ttcaccgcca ccagcgcctt acccccttcc ggccacggct
     6781 ggtctttcac catccacacg gggcttgggc atttgcgtaa cagatgccag tccgttggcg
     6841 taaaaatcac cgcttccaga cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc
     6901 cgccgctgat cacttcctga atgatggctt cgaaaggacg gttatgccag accactttaa
     6961 tttcaatggg aacgccagca ttgagataat attttgcctg ctcgtggatc caggctgtac
     7021 gctggctgat gacgccctga cgcatagcgg tacgttcgtc cggagagagc agggtggtca
     7081 tttcgtatga gaagtcatag atcggcaaaa aggctttaat tttgccacca atccgttgat
     7141 gtaaataaac agctcgccgc aatgctggtt ggtcgtcctg gttaggatcg ataacaacga
     7201 gcatgttctg atacatagcc atacagggtc tccttacaac aactgtcaac gcagtttgta
     7261 attaaaagat taacccatat ctggtgaatg aaacagtgat gaaccttctg tcagatcaat
     7321 aaatcagaaa aatttaatga tatgacagaa ggatagtgag ttatgcggaa gaatcaggca
     7381 acgttacgcg tgtgaccagc aagctgggcc agcgcatcgt tattttcgat agtgatgtat
     7441 ttacctttga ctgccagcat accgcttttc tggaagcgac ccagcagacg gctgatggtt
     7501 tcaaccgtca ggcccagata gttaccgata tcaccacgag tcatcgtcag gcggaattca
     7561 cgaggggaga agccgcgttg ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga
     7621 cgttcctcgg catttttctt cgacaacagc aggatcatgt cctgatcgcc tttgatttca
     7681 ccgctcatca gacgcatcat ctgctgacgc agattcggca ttttaccaga caaatcgtcc
     7741 agcgtttcga acgggatttc acataccatc gaggtttcca gcgcctgcgc gaaactcggg
     7801 tgatgaccgc tgccgatggc atcaaatccc accagatcgc ctgctaaatg gaaaccagtg
     7861 atttgctcgt cgccttgctc agtgatggta taacttttaa tcgtaccgga gcggatggca
     7921 taaagcgatt taagttcatc tccagcctta aacagcgtct ggcctttctg aataggcttc
     7981 ttccgctcaa tgatattatc aagctgatca agctcatgtt cgttgagtgt gaacgggatg
     8041 caaagctggc tgatgctgca atcctggcaa tggatagcac aaccgccaga ctgaatgcgc
     8101 cgtataattc gcttttccgg gatcataggt ctgctcaagc cgtaattgat atttgtcaat
     8161 tttaacatct ttttagggag caagtaagtc taagcaaacc ttaacagcag agaattccga
     8221 tattagatgt aaatatatgt ctatctattt gaaaaccctt aagttgttaa gggtgacttt
     8281 acataaaagt gtgaacaagc tggcacaaat tgtttaatgt ttacagcaaa agataacctt
     8341 cgtggcgcaa taaccactct tttcgctgaa ctccgcctgc atatccggtc atggtgccgt
     8401 ttcggccaat aacccgatgg caaggtacga cgatgctgat gggattcgat ccgtttgccg
     8461 caccaacggc acgcgccgcg ccagggcggc ctaattgttc agccagttgg ccataatgca
     8521 ttacctgccc gcaggggata gtgcgcagtg ttttccagac ttcgcgctga aatggcgtcc
     8581 cccccgtggc agtgggaagc gtatcaataa tgctaagatt accggcaaaa tattcacgca
     8641 gcttgtcgct taaaccacct gggttggtgg cagaaatgcg ctcatagcct tctttgcgat
     8701 aatggatgtc cagcagctgc accatgcgtt cgctgtactc ttcccattca accgcccgca
     8761 ggcgaaattg ctcatcgcaa atcacccaca gtggacccag tggcgtggca attttttctt
     8821 caagtaatct cagcatccgt tctctcttaa gacaaacgtg ggtaaatacc cggacctatc
     8881 ggcaagccaa caagatacca cgccagcaac atcagcagcc ataccaccaa aaagataagt
     8941 ggatagggta agactaacga atagtaagta cccagtttcg cgtctggttt gtcgcagtct
     9001 gatagtatta agcgattttt tgatttttct gcctgaagaa atgagggcaa gccatcacat
     9061 ggatcgaggg tgggataatt ccagtcagcg aaattttacc gtaggttatc tgttatttga
     9121 tctgtggcgt gatagccgcc agacaccact gatctcgtca ctcattcccc aatcccggcg
     9181 agaatgtggt tatttacagg gctaaaaaat gaataaaaaa atagagcctg tcgacatccg
     9241 ccagactcta cagtacacac agcagtgcat cctcgtctta aatccggcct ggggtcctgg
     9301 tgaccggtga cagtgttata acggttttac ccatccgttg ccaattttct tcagtgacag
     9361 atttcacgaa aatataagtt aaatcatttg tcttgtgatt gcataatcat gcatcgttaa
     9421 tcgacgagta aatttaccgg gcgagtagcc cgttcgacgc ttaaacatat tgccgaacga
     9481 ttcagctgag gcatagccca gacgatgggc aattttggtc aacgaatatc catcatcaag
     9541 aagcgtcact gcagaaataa cgtgcatttg ctgaacccag tggcgaaatg ttaatcccgt
     9601 ctctttaaca aataagcgca tgagtgtacg gctactgacg ttaattttat ccgctagatt
     9661 atccagtgtt ggcagatacc cttggttgtt tagcaaattc tcacaaagct gctgtagccg
     9721 ctcatcattt ggccaaggga gttcaagtga cattttcacc cccattttta tttcctgaag
     9781 tatcagtgtc tcgagggctg aaaaacgtaa ctgttgttta tctgtggtac tttgcgacgg
     9841 tggaagttgg ttaattgcaa ttagtaattc ccttaacagg ggtgaaatac ccgtcatgaa
     9901 gcaaactttt cccagcgttt cactttccgc ttctgaaacc agtacggttt ttaaaagtac
     9961 gttgcctgtc gcaacaaact tatgcggcat ttgtgggggc agccaaagcg cgctgctggc
    10021 atgcactagc cagacgcttt ctccagccca tacacgaacg actccttcag ccgggtaaag
    10081 caactgggca tagagatgtt tatgccaggg aactgtacct ccattcagat gttccgaatc
    10141 cagtccgata atgtgatgcc ctggtgatgc tttgggttgc agtgtgattg tcatttacag
    10201 taggtatttt gtcagaaatc atcaaataag ccatgttaaa ttttaaatat gacgtatgca
    10261 agtttccgga tatatacggt gtaaagagtg atgataattt ctaaaaaaca gcttattggg
    10321 gtggtagcga tcggtattct tttggctggc gttgtctttt ttatctggtg ggtaagcaaa
    10381 ggaagattta tacagacgac ggatgatgct tacattggtg gtaatatcac cacggtgaca
    10441 agtaaagttt ctggttatat ttctgctata gaggtgcgcg ataatcagag tgtaaaaaaa
    10501 ggcgatatta tcctgcgtct cgatgatcgg gattatcgtg caaatgtcgc caggcttgag
    10561 gcaaaaataa aaagtagcaa agccaatctc gaaagcattc aagccactat tgcgatgcag
    10621 cagtcgataa ttcaaagtgc cagtgaaaca tggcaggcag taaaacatga ggagcaaaag
    10681 cgcctgcgag atactgaacg ttatgaaaaa ctcgcacaat cagcggcaat atcgcaacaa
    10741 attattgata acgccaggtt tgactaccag caggtcgcag caaaggagcg caaagccgcg
    10801 aacgatttcc tggtggagaa gcaacggctg gcagttttat ctgctcagga agaaaacgta
    10861 cgtgcaagta tcgaagaagt tcaggccgct ttgactcagg cactactgga tctggaatat
    10921 acccttgttc gtgcgccgat tgatggcatt gtcgcaaacc gcagcgccca tacaggcagt
    10981 tgggtggaag gcggcacatc gttagtttca ctagtaccag tcagtgagtt gtgggttgat
    11041 gcaaattata aagaaaacca aatagcggga atgaagccag gaatgaaggc tgagatccgt
    11101 gctgatattc tcaaagggga agtgtttcac ggtcacattg aaagcctttc cccagcgaca
    11161 ggagccagtt tcagcttgat tccgatagaa aatgccactg gcaatttcac caaaattgtt
    11221 cagcgtgtcc cggtacggat tgcatttgac gacgcaaagg aattaaagca acttcttcgc
    11281 ccgggactgt ccgtcaccgt cagtgttgat gagagatgat gtctatgcgt aaacatattg
    11341 ctttcgccag tatgtgtatg ggattattta ttgctcagct tgatattcaa attgtatcgt
    11401 catccctaaa tgaaataggt ggtggtctgt ccgcaggtaa agacgaaatg gcctggcttc
    11461 agaccagcta tttaattgct gagattattg ttattccgct ctctgggtgg ctgtcacggg
    11521 tattttccac tcgttggctt tttactctat ccgcgggaat atttacttta atgagtatcg
    11581 cgtgtggact tgcttggaat attcagatca tgattttttt tcgtgcttta cagggagctg
    11641 ctggcgcatc catgatccct ttagttttca cgacggcgtt tatttattac cagggtaaag
    11701 agttggggtt ggctgctgcg gtagtcagtg cgctggcctc actgtcaccg acgctagggc
    11761 caactctggg gggctggatc actgataacc tcgactggcg atggctattt tatatcaata
    11821 tattacctgg catatattta gttttgagta ttccttttct ggttaatttt gataaacctg
    11881 atctctcttt actgaaagtt gctgattatc cgagtattat cctgttggcc atgacactgg
    11941 gatgtctgga atataccctt gaagaaggag cgcgctgggg atggctggat gataatacta
    12001 tttttctgac atcagttctg gcattggtga gttttatttt atttgctgcg cgtacactta
    12061 caatatccaa tccgataatg gatcttcatg cttttaagga taa
//
LOCUS       DACADA010000135        12032 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.137, whole genome shotgun sequence.
ACCESSION   DACADA010000135 DACADA010000000
VERSION     DACADA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.137"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            67..450
                     /locus_tag="ISB17_003677"
     CDS             67..450
                     /locus_tag="ISB17_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981567.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(514..957)
                     /locus_tag="ISB17_003678"
     CDS             complement(514..957)
                     /locus_tag="ISB17_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP1981568.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ISB17_003679"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ISB17_003679"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAP1981569.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="ISB17_003680"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="ISB17_003680"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAP1981570.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="ISB17_003681"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="ISB17_003681"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAP1981571.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5372..7096
                     /gene="aceK"
                     /locus_tag="ISB17_003682"
     CDS             5372..7096
                     /gene="aceK"
                     /locus_tag="ISB17_003682"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAP1981572.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ISB17_003683"
     CDS             complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ISB17_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAP1981573.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8138..11821
                     /gene="metH"
                     /locus_tag="ISB17_003684"
     CDS             8138..11821
                     /gene="metH"
                     /locus_tag="ISB17_003684"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP1981574.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2932 a         3070 c         3228 g         2802 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acagtggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaatccaa tcggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggcggtaca ggccgcgcta
     1561 aatatcctct acggcattcc taagcaaact cgcaccgaca aactctctgg cgtttatgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag taagtgaata aattttattt atattgttat
     2161 caacaagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttc acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaaggttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcagt taacggcacc atcagctata ccaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgaggca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccgagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgtgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccaggggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggcggtcag gcgttgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagctg tggtagagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggtga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt ggggacaggt
     5161 tatttcgata aagtgacgac cattattcag ggcggcacgt cttcagtcac cgcgctgacc
     5221 ggctccaccg aagaatcgca gttctaagca actgataaag ttgatgattg taggccggat
     5281 aaggcgttca cgccgcatcc ggcaatcaac gcctgatgcg acgatttgcg cgtcttatcc
     5341 ggcaattctc tgctcctgat gagggcgcta aatgccgcgt ggcctggaat tactgattgc
     5401 acaaaccatt ctgcaaggct tcgacgctca gtatggtcga ttcctcgaag tgacttccgg
     5461 ggcgcagcag cgtttcgaac aagccgactg gcacgctgtc cagcaagcga tgaaaaaccg
     5521 tatccatctt tacgatcatc acgtgggtct ggttgtggag caactgcgct gcattactaa
     5581 cggccaaagc acggacgcgg catttttact gcgcgttaaa gagcattaca cccgactgtt
     5641 gccggattac ccgcgcttcg agattgcgga gagctttttt aactccgtgt actgtcggtt
     5701 atttgaccac cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg
     5761 ccgttttcgt accattcccc gcccgctggc gaaagacttt caccccgatc acggctggga
     5821 atctctgctg atgcgcgtta tcagcgacct gccgttgcgt ctgcgctggc agaataaaag
     5881 ccgtgacatt cattacatcg ttcgccatct aacggaaacg ctggggacag acaacctcgc
     5941 ggaaagtcat ttacaggtgg cgaacgaact gttttaccgc aataaagccg cctggctggt
     6001 gggcaaactg atcacgcctt ccggcacatt gccatttttg ctgccgatcc accagacgga
     6061 cgacggcgag ttatttattg atacctgtct gacgacgacc gccgaagcga gcatcgtctt
     6121 tggctttgcg cgttcttatt ttatggttta cgcgccgctg cccgcagcgc tggtcgagtg
     6181 gctacgggaa attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca
     6241 gaagcacgcc aaaaccgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga
     6301 gcagttcatt gaagcgccgg gtattcgtgg aatggtgatg ttggtgttca cgctgccggg
     6361 ctttgaccgg gtatttaaag tgatcaaaga caagttcgcg ccgcagaaag agatgtctgc
     6421 cgctcacgtt cgcgcctgct accaactggt gaaagagcac gatcgcgtgg gccgaatggc
     6481 ggacacccag gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat
     6541 ggcattactg ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac aaattgtgat
     6601 tcgccatctt tatattgagc ggcggatggt gccgctcaat atctggctgg aacaagtgga
     6661 aggtcagcag ttgcgcgatg ccattgaaga atacggtaac gctattcgcc agcttgccgc
     6721 tgctaacatt ttccctggcg acatgctgtt taaaaacttc ggtgtcaccc gtcacgggcg
     6781 tgtggtgttt tatgattacg atgaaatttg ctacatgacg gaagtgaact tccgcgacat
     6841 cccgccgccg cgctacccgg aggacgaact tgccagcgaa ccgtggtaca gcgtctcgcc
     6901 gggcgatgtt ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc
     6961 gctatttgaa gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa
     7021 ccgtatacgt gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag
     7081 cgttcgattc gtataaatca gaggcgaaaa agtcagcgta ttccaccgta tgccagcgtc
     7141 acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc atcggtaata
     7201 cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata
     7261 cacgctgcaa ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt
     7321 ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt tgcgtgggta
     7381 taggcatgta accctttgcg gtgcagtaac ttcgtcacct gttcttcgct cagttgggcc
     7441 agaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac
     7501 attcgcatca ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg attgctttga
     7561 tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg
     7621 tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt
     7681 gcgccgatcg cccagtgacc cagttcgccg acctgacgga caaagccctg ttgctgcatc
     7741 gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgctgtgc cagttccgtg
     7801 agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa
     7861 gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg
     7921 ggaatgggtg cgaccatgac agtctccttt tttctgtatc gtggaaatca ttttcatttt
     7981 tattatcagc gaatgcaata gttgctgaac agatccgatg agttaatgtt gaacaaatct
     8041 catgttgcgt ggtggtcgct tttaccacag atgtgtttat gccagtatgg tttgttgaat
     8101 ttttattaaa tctgggttaa gcgtgtcggg agcaagtgtg agcagcaaag tggaacaact
     8161 gtgtgcgcag ttaaatgaac gtattctggt gctggacggc ggtatgggca ccatgatcca
     8221 gagctatcga ctgaacgaag ccgattttcg tggtgaacgc tttgccgact ggccatgcga
     8281 tctcaaaggc aacaacgacc tgctggtgct cagcaaaccg gaagtgatct ccgccatcca
     8341 caacgcttac tttgaagcgg gtgcggatat catcgaaacc aacaccttca actccacgac
     8401 tattgcgatg gcggattacc agatggaatc cctgtcggcg gaaatcaact ttgcggcggc
     8461 gaaactggcg cgcgcctgtg ccgacgagtg gactgcccgc acgccggaga aaccgcgcta
     8521 cgttgccggt gttctcggcc cgaccaaccg cacggcgtct atttctccgg acgtcaacga
     8581 tccggcattc cgcaacatca cttttgacca gctggtggcg gcttatcgag aatccaccaa
     8641 agcgctggtg gaaggtggcg cggacctgat cctgattgaa accgttttcg acacccttaa
     8701 cgccaaagcg gcggtctttg cggtgaaaac ggagtttgaa gcgctgggcg ttgaactgcc
     8761 gattatgatc tccggcacca tcaccgacgc ctccgggcgc acgctctccg ggcagaccac
     8821 tgaagcgttt tacaactccc tgcgccacgc cgaagcgctg acctttggcc tgaactgtgc
     8881 gctggggcca gacgaactgc gccagtacgt acaggaactg tcgcgtattg cggaatgcta
     8941 tgtcaccgcc cacccgaacg ccgggctgcc caacgccttt ggtgaatacg atctcgacgc
     9001 cgacacgatg gcaaaacaga tacgtgaatg ggcagaagcg ggctttctca atatcgtcgg
     9061 cggctgctgc ggcaccacgc cacaacatat tgcagcgatg agtcgtgcag tagaaggatt
     9121 agcgccgcgc aaactaccgg aaattcccgt agcctgccgt ttgtccggcc tggagccgct
     9181 gaacattggc gaagatagcc tgtttgtgaa cgtgggtgaa cgcaccaacg tcaccggttc
     9241 cgctaagttc aagcgcctga tcaaagaaga gaaatacagc gaggcgctgg atgtggcgcg
     9301 tcagcaggtg gaaaacggtg cgcagattat cgatatcaac atggatgaag ggatgctcga
     9361 tgccgaagcg gcgatggtgc gttttctcaa tctgattgcc ggtgaaccgg atatcgctcg
     9421 tgtgccgata atgatcgact cctcgaaatg ggacgttatt gaaaaaggtc tgaagtgtat
     9481 ccagggcaaa ggcattgtta actctatctc gatgaaagag ggcgtcgacg cctttatcca
     9541 tcacgcgaaa ctgttgcgtc gctacggtgc ggcagtagtg gtaatggctt ttgacgaaca
     9601 gggccaggcc gatacccgcg cacggaaaat cgagatttgc cgtcgggcgt acaaaatcct
     9661 caccgaagag gttggcttcc cggctgaaga tatcatcttc gacccgaaca tcttcgcggt
     9721 cgcaactggc attgaagagc acaacaatta cgcacaggac tttatcggcg cgtgtgaaga
     9781 catcaaacgt gaactgccgc acgcactgat ttccggcggc gtttctaacg tgtcgttctc
     9841 gtttcgtggt aacgatcctg tgcgcgaagc cattcacgcg gtgttcctct actacgctat
     9901 tcgcaatggc atggatatgg ggatcgtcaa cgccgggcaa ctggcgattt acgacgacct
     9961 gcctaccgag ctacgcgacg cggtggagga tgtgatcctc aaccgccgcg acgatggcac
    10021 cgagcgttta ctcgagcttg ccgagaaata tcgcggcagc aaaaccgacg acaccgctaa
    10081 cacccagcag gcggagtggc gctcgtggga agtgaataaa cgtctggaat actcgctggt
    10141 caaaggcatt accgagttta tcgagcagga taccgaagaa gcccgccagc aggctacgcg
    10201 cccgattgaa gtgattgaag gcccgttgat ggacggcatg aacgtggtcg gcgacctgtt
    10261 tggcgagggc aaaatgttcc tgccgcaggt ggtgaaatcg gcgcgcgtaa tgaagcaggc
    10321 ggtggcgtat ctcgagccgt ttattgaagc cagcaaagag cagggtaaaa ccaacggcaa
    10381 gatggtgatc gccacagtga agggcgatgt ccacgacatc ggtaaaaata tcgttggcgt
    10441 ggtgctgcaa tgtaacaact acgaaattgt cgatctcggc gtgatggtgc ctgcggaaaa
    10501 aattctccgt accgctaaag aagtgaatgc tgatttgatt ggcctttcgg ggctgatcac
    10561 gccgtcgctg gacgagatgg ttaacgtggc gaaagagatg gagcgccagg gcttcaccat
    10621 tccgctattg attggcggcg cgaccacctc aaaagcgcac acggcggtga aaatcgagca
    10681 gaactacagc ggcccgacgg tgtatgtgca gaacgcatcg cgcaccgttg gtgtggtggc
    10741 ggcgctgctt tccgacactc agcgcgatga tttcgttgct cgtacccgca aggagtacga
    10801 aaccgtgcgt attcagcacg gacgaaagaa accacgcaca ccaccggtca cgctggaagc
    10861 ggcgcgtgat aacgacttcg cttttgactg gcaggcttac acgccgccgg tggcgcaccg
    10921 tctcggtgtg caggaagtcg aagccagcat cgaaacgctg cgtaattaca tcgactggac
    10981 gccgttcttt atgacctggt cgctggccgg gaagtatccg cgcattctgg aagatgaagt
    11041 ggtaggtgtt gaggcgcagc ggctgtttaa agacgccaac gacatgctgg ataaattaag
    11101 cgccgaaaaa atgctgaacc cgcgtggtgt ggtgggcctg ttcccggcaa accgtgtggg
    11161 cgatgacatt gaaatctacc gtgacgaaac gcgtacccat gtgattaatg tcagccacca
    11221 tctgcgccaa cagaccgaaa aaaccggctt cgctaactac tgtctcgctg acttcgttgc
    11281 gccgaaggtt tccggcaaag cggattacat cggcgcattt gccgtgactg gcgggctgga
    11341 agaggacgca ctggctgatg cctttgaagc acagcatgat gattacaaca aaatcatggt
    11401 gaaagcgctt gccgaccgtc tggcggaagc ctttgcggag tatctccatg agcgtgtgcg
    11461 taaagtctac tggggctatg cgccgaacga gaacctcagc aacgaagagc tgatccgcga
    11521 aaactaccag ggcatccgtc cggcaccggg ctaccctgcc tgcccggaac atacggaaaa
    11581 agccacaatc tgggagctgc tggaagtgga aaaacatacc ggtatgaaac tcacggaatc
    11641 tttcgccatg tggccgggtg catcggtttc gggttggtac ttcagtcatc cagacagcaa
    11701 gtattacgcg gtggcgcaaa ttcagcgcga tcaggttgaa gactatgccc accgtaaagg
    11761 aatgagcgtt accgaagttg agcgttggtt ggcaccgaat ctggggtatg atgccgagta
    11821 agtgataaca ccggggtatt tgccccggtt tttaatgtca taaataatgg attattgaat
    11881 atgataaaaa ttgatgaaat agattttttc gctttcgaag gatgttcaga gtccgaagtt
    11941 actgaactat taggaaaaaa ccaacaggtt ataatgattg gataattaat ataaaacagg
    12001 tgtggagtgg gtattttatg atggtcgtta tt
//
LOCUS       DACADA010000136        11925 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.138, whole genome shotgun sequence.
ACCESSION   DACADA010000136 DACADA010000000
VERSION     DACADA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.138"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..868
                     /locus_tag="ISB17_003685"
     CDS             <1..868
                     /locus_tag="ISB17_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981575.1"
                     /translation="AISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELRD
                     LLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLL
                     DFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKTR
                     EATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
     gene            complement(918..1271)
                     /locus_tag="ISB17_003686"
     CDS             complement(918..1271)
                     /locus_tag="ISB17_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP1981576.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1409..3055)
                     /gene="groL"
                     /locus_tag="ISB17_003687"
     CDS             complement(1409..3055)
                     /gene="groL"
                     /locus_tag="ISB17_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP1981577.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3099..3392)
                     /locus_tag="ISB17_003688"
     CDS             complement(3099..3392)
                     /locus_tag="ISB17_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP1981578.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3668..4924
                     /gene="yjeH"
                     /locus_tag="ISB17_003689"
     CDS             3668..4924
                     /gene="yjeH"
                     /locus_tag="ISB17_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP1981579.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4940..5416)
                     /gene="fxsA"
                     /locus_tag="ISB17_003690"
     CDS             complement(4940..5416)
                     /gene="fxsA"
                     /locus_tag="ISB17_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAP1981580.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5753..7189
                     /gene="aspA"
                     /locus_tag="ISB17_003691"
     CDS             5753..7189
                     /gene="aspA"
                     /locus_tag="ISB17_003691"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAP1981581.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7307..8608
                     /locus_tag="ISB17_003692"
     CDS             7307..8608
                     /locus_tag="ISB17_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP1981582.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8724..9062
                     /gene="cutA"
                     /locus_tag="ISB17_003693"
     CDS             8724..9062
                     /gene="cutA"
                     /locus_tag="ISB17_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAP1981583.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9038..10735
                     /locus_tag="ISB17_003694"
     CDS             9038..10735
                     /locus_tag="ISB17_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAP1981584.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10772..11347
                     /locus_tag="ISB17_003695"
     CDS             10772..11347
                     /locus_tag="ISB17_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1981585.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11454..11529
                     /locus_tag="ISB17_003696"
     tRNA            11454..11529
                     /locus_tag="ISB17_003696"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11487..11489,aa:Phe,seq:gaa)
BASE COUNT         2873 a         3082 c         2927 g         3043 t
ORIGIN      
        1 ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc
       61 actggcgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgccat
      121 ggaattgcgc gaccttctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga
      181 tgctgctgca cgccttcagt atgaacaagc tcgcgataaa gtcattaaga aaatggcaga
      241 aaatatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa
      301 cacgctggaa cttaccgatg atcagggcga aaatcttacc ttcgtattaa cactacatga
      361 cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat
      421 cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt
      481 agatttcctg ccgttatatg atgtcgactg ccaggatgat ggtaatctgg aatatgacac
      541 ctattcccaa ccggaatgga aacataactt gttcaaccat tatctggcgg tgctttaccg
      601 ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc
      661 aacgccgggt aaagaagtgg aggcgatcac tcgccgaatg cttgatttca gcccccgact
      721 gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg caaataacgc
      781 ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac
      841 cagcaaaact gcaccacagg cgaaataatc ttatctgttt tactacgagg ctggaagtat
      901 ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat ctggctatcc
      961 gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta
     1021 ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgcct
     1081 tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgttgcagt accaatcagc
     1141 tggcactctg cacctggttg ctcgtccaca atgcgtacac tctgaccggc agcactcaat
     1201 tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat
     1261 tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc
     1321 tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt
     1381 ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc
     1441 ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg gtaaccatgc
     1501 actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta
     1561 ctttggttgg atccaggata cccatatcga tcatgttgcc gtattcttca gttgctgcgt
     1621 tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc
     1681 cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac
     1741 ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca
     1801 gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg
     1861 cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca ccaactttga
     1921 taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt
     1981 cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt
     2041 cttcacccac gccatcgatg atggtggtgg tgtctttgtt gatcacaaca cgtttagcct
     2101 gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg
     2161 taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg
     2221 gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca
     2281 gtgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttacctgct ttggcaacag
     2341 cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga
     2401 acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaagt
     2461 agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt
     2521 cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac
     2581 ctacggtttc gtcggagtta gcagagatgg taccaacctg agcaatcgct ttagagtcag
     2641 agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct ttgtcgatac
     2701 cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagaccc tcagtgatga
     2761 tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtca ttcgctttag
     2821 aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc agttcgattt
     2881 cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa
     2941 ctacgttacg gcctttagga ccgagggtaa ctttcactgc atctgccagt acgtttacgc
     3001 cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct
     3061 ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga
     3121 atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg
     3181 tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca
     3241 ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct
     3301 gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg
     3361 atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta
     3421 tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg
     3481 atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg
     3541 gtgaaaaccc tgattcacct cacatttcat cgctatttct tcatcaccgt cataagcaaa
     3601 tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggaca
     3661 tcaacccatg agtggactca aacaagaact ggggctggcc cagggcatcg gcctgctatc
     3721 gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg ttagctgcgc tagtagcagg
     3781 caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt
     3841 gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc gtcgcgcact tcgtcggtat
     3901 ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt
     3961 gggtttgcct gccgcgctac aaattgctgc cggattcggt caggcaatgt ttggctggca
     4021 tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac
     4081 tcgcggtgcc agttccagtg ctaatctaca aacagttatt gccggactta tcgtcgcact
     4141 gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct tccctgcgcc
     4201 aggaaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt
     4261 cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc
     4321 tcgtgctttg atgattggcc tgctgctggc aggattagtc tattggggct gtacggtagt
     4381 cgtcttacac ttcgacgcct atggtgaaca aatggcggcg gcagcatcgc ttcccaaaat
     4441 tgtagtgcag ttgttcggtg taggagcgtt atggattgcc tgcgtaattg gctatctggc
     4501 ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc
     4561 gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc
     4621 cctcaatgcg gtgctcggct gctgcgtggt aagcacgttg gtgattcatg ctttagagat
     4681 caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg
     4741 catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg
     4801 gctattatgc gttctgttac tggcaatggt cggctggaaa agtctctacg cgctgatcat
     4861 gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac
     4921 ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg cggtcgcggt
     4981 catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa
     5041 aaccgccgcc tggcatgcga gaaaagcgca aatgcggcat caacttcact gtcagatgct
     5101 tttgcaccgg cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata
     5161 aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt
     5221 cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca
     5281 gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata
     5341 catgagcaac ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa
     5401 aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaacccg gctacctgaa
     5461 tgggttgcga atcgcgttta gcttatattg tggtcattag caaaatttca agatgtttgc
     5521 gcaactattt ttggtagtaa tcccaaagcg gtgatccatt tcacaaatta ataattaagg
     5581 ggtaaaaacc gacacttaaa gtgatccagg ttacggtaga aatcctcaag cagcatatga
     5641 tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat
     5701 atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa
     5761 caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta
     5821 tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga
     5881 tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa
     5941 agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt
     6001 cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg
     6061 tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg
     6121 tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc
     6181 tactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaaact
     6241 ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga
     6301 catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga
     6361 attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga
     6421 actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa
     6481 agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc
     6541 ggctgaagac ctgatcgaag cgacctccga ctgcggcgct tatgttatgg ttcacggcgc
     6601 gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc
     6661 tggtccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcag gctcttccat
     6721 catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt
     6781 catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt
     6841 tatggagcca gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg
     6901 ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg
     6961 ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa
     7021 cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct
     7081 ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat
     7141 gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt
     7201 agtacaaata aagaaggcac gtcagatggc gtgccttttt tcttgtgagc agtaacttaa
     7261 aaataacaat ttaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
     7321 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
     7381 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
     7441 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
     7501 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
     7561 atacatcacg atcctcgcac cgatcgtgac ctatttcctg actatctttg ctggtactgg
     7621 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
     7681 accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
     7741 aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct
     7801 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct
     7861 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
     7921 agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
     7981 gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag
     8041 cccaagcatg ggtctggttg aaaaaccact gatgaacacc accaacgcaa tcctgatcat
     8101 catgctcagt gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacattct
     8161 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg
     8221 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
     8281 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
     8341 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc
     8401 gctgaccgct gttgcttctt ttgctgcggt gtctggtctg ttcattctgc cgacctaccc
     8461 gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt
     8521 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg
     8581 cttcgtgctg ggtagcttca tgctgtaatg acccatcgcg gggcgttcac gccccgcttt
     8641 ctttcccgcc gactaacatc ctttccccgt ccgttgtata gtgacctctc tcttgcagtt
     8701 ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg
     8761 tggtgctatg tacggcaccg gatgaagcga cagcccagga tttagccgcc aaagtgctgg
     8821 cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg
     8881 aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc
     8941 agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg
     9001 ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct
     9061 gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc
     9121 acaatttgtc cccgcggatc aagcctttgc ttttgatttt cagcaaaacc aacatgacct
     9181 taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac
     9241 gccggaacac gcgaaaattg ctgacgtgca gctgccgcaa ggcgtctggc atgaagatga
     9301 gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca
     9361 ggcaagtgcg ggagcaacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg
     9421 ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgaagcgtc
     9481 acagcctgtg tctgttccgc agcaagagca gcccaccgcg caattgccct tttccgcgct
     9541 ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc
     9601 actgatttct ggcatcgtgc tgggcggtaa acagcggctt tccactgcca gagcattgtt
     9661 gctgaccttt atttatgtgc aggggatggc gctgacttac acggcgctgg gtctggtggt
     9721 tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct
     9781 cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tgcaactccc
     9841 ctcttcgctg caaacacgtc tcacgctgat gagcaatcgc caacagggcg gctcacctgg
     9901 cggtgtgttt attatggggg cgattgccgg actgatctgt tcaccatgca ccactgcacc
     9961 gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcac
    10021 gctttatctt tatgcgctgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa
    10081 ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt
    10141 gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatatat ggggattacg
    10201 cttgtggtcg gcgcttggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc
    10261 caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt
    10321 acgcccactt caggattggg catttggtgc aacacatacc gcgcaaactc agacgcatct
    10381 caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg aagccaaagg
    10441 caaaccagtg atgttagatc tctatgccga ctggtgcgtc gcctgtaaag agtttgagaa
    10501 atacaccttc agcgactcgc aggtgcaaaa agcgttagca gatacggtct tacttcaggc
    10561 caacgtcact gccaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg
    10621 cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac
    10681 gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac
    10741 acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc
    10801 cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg
    10861 tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta
    10921 cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgctgga
    10981 cgaaacgcaa accgccgaac aaaagttgct ggcacgttat caggcgttat cggagtgcgt
    11041 taaaaacaac cgctatccgg gctgtctgtt tatcgctgcc tgtacgtttt atcccgatcc
    11101 tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg atttcaccca
    11161 cgaactgtta accacgctgg aagttgacga tccggcgatg gtagcaaagc agatggaact
    11221 ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac
    11281 cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgtcagg gtggtgcact
    11341 gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt
    11401 ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga
    11461 tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc
    11521 cgggcaccaa attcatataa acggacctcc acggaggtcc atttttcgtt tcaggacgcc
    11581 acgatttaag cgtcctgccg ccaaatcaat tctaccgaac tcaaccagat tctccccaca
    11641 tcaccagcaa tttgcgggca tatcccaatt cgggaaaatt tgtttctgag ctatagcgct
    11701 gactgacgtg aaatgtcgtg cggccccgtg atgctgttga acgtcaaatg acgtcatcag
    11761 gagcgtaatg catccataaa gcacaacatc gggcagaacg ccaactgatg agattttctg
    11821 aatgagaaca aagagaaatg tatcagtccg tttgctcatg caaagactaa caatccatta
    11881 aaatagtaag cgctccggac aatttttcat ggattatttt ctgaa
//
LOCUS       DACADA010000137        11733 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.139, whole genome shotgun sequence.
ACCESSION   DACADA010000137 DACADA010000000
VERSION     DACADA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.139"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..583
                     /locus_tag="ISB17_003697"
     CDS             <1..583
                     /locus_tag="ISB17_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAP1981586.1"
                     /translation="EYEIGDIVFTCIGAALFGQISAASNCWNNHVGIIIGHNGEDFLV
                     AESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGF
                     KYESSRQFCSKFVFDTYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWER
                     KTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(585..1106)
                     /gene="ruvC"
                     /locus_tag="ISB17_003698"
     CDS             complement(585..1106)
                     /gene="ruvC"
                     /locus_tag="ISB17_003698"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAP1981587.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1141..1881)
                     /locus_tag="ISB17_003699"
     CDS             complement(1141..1881)
                     /locus_tag="ISB17_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1981588.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1910..2362)
                     /gene="nudB"
                     /locus_tag="ISB17_003700"
     CDS             complement(1910..2362)
                     /gene="nudB"
                     /locus_tag="ISB17_003700"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAP1981589.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2355..4127)
                     /gene="aspS"
                     /locus_tag="ISB17_003701"
     CDS             complement(2355..4127)
                     /gene="aspS"
                     /locus_tag="ISB17_003701"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP1981590.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4437..5003
                     /locus_tag="ISB17_003702"
     CDS             4437..5003
                     /locus_tag="ISB17_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1981591.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5000..5818
                     /locus_tag="ISB17_003703"
     CDS             5000..5818
                     /locus_tag="ISB17_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP1981592.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRYAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5871..6266
                     /locus_tag="ISB17_003704"
     CDS             5871..6266
                     /locus_tag="ISB17_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981593.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6307..7050
                     /gene="cmoA"
                     /locus_tag="ISB17_003705"
     CDS             6307..7050
                     /gene="cmoA"
                     /locus_tag="ISB17_003705"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAP1981594.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7047..8018
                     /gene="cmoB"
                     /locus_tag="ISB17_003706"
     CDS             7047..8018
                     /gene="cmoB"
                     /locus_tag="ISB17_003706"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAP1981595.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8054..10483)
                     /locus_tag="ISB17_003707"
     CDS             complement(8054..10483)
                     /locus_tag="ISB17_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP1981596.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAIQA"
     gene            complement(10508..11608)
                     /locus_tag="ISB17_003708"
     CDS             complement(10508..11608)
                     /locus_tag="ISB17_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAP1981597.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDSALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2684 a         3103 c         2869 g         3077 t
ORIGIN      
        1 ggaatatgaa attggtgata tcgtctttac atgtataggt gctgccttat ttggtcaaat
       61 atcagctgca tcaaattgct ggaataatca cgtcgggatc attatcggtc ataacggtga
      121 agactttctg gttgcagaaa gccgtgttcc cctctcaacc atcactacgc tatcccgttt
      181 tattaaacgc tctgctaatc aacgctatgc tataaagcga ttagacgccg gactaacaga
      241 acaacaaaaa caacgaattg ttgaacaggt tccttcccgg ctacgcaaac tttaccacac
      301 cggttttaaa tacgaatctt cgcgacagtt ctgttcaaaa tttgtttttg atacttataa
      361 agaggcgcta tgtattccgg tgggtgaaat agagacgttt ggagaattat taaatagcaa
      421 tccgaatgca aaactcactt tctggaaatt ctggttctta ggttctattc cgtgggagcg
      481 taaaaccgtc acgccagcca gtttgtggca tcatccgggt ttggtgttga ttcacgcggt
      541 gggagttgaa acgcctcagc ctgaactgac cgaggcggta taacttaacg cagtcgccct
      601 ctcgccaggt tcagtcgcga ttcactcatt tgcatcgcat tctgactaac gtggcagtgg
      661 gtgatggcaa tcgccagcgc atcggcggca tccgcctgtg gattagcggg cagtttcagc
      721 aaggtgcgga ccatatgctg cacctggctt ttttcggcac taccaatacc taccactgtt
      781 tgctttacct gacgtgccgc atattcaaat accggcaatt cctgattcac cgccgccaca
      841 atcgccacgc cgcgcgcctg ccccagtttc agggctgagt cagcgttctt cgccataaag
      901 acttgttcaa tggcgaaata atcaggctgg aattgggtga tgatttccgt cacgcccgca
      961 tagatgagct tcagacgaga cggtaaatca tccactttgg tgcgtatgca tccgctaccc
     1021 aggtaggaca gttgcctgcc tacctggcgg atgacgccat agccggtcac gcgcgaaccc
     1081 gggtcaatac cgagaataat agccatcacg cgtctccgtt ttgctgttta gcaggcctca
     1141 tcagagagtc gctgcaacct catcagagat ttcaccgtta tggtaaactt cctgcacgtc
     1201 gtcgcaatct tccagcatat cgatcagacg catcagtttc ggtgcggttt ctgcatccat
     1261 atcagctttg gtggacggga tcatggaaac ttccgcgctg tctgctttca gacctgccgc
     1321 ttccagagcg tcgcgtactt tgcccatttc ttcccatgca gtgtagacat caatcgcgcc
     1381 gtcatcatag gtcacaacgt cttcagcacc ggcttccagg gctgcttcca tgatggtgtc
     1441 ttcatcgcct ttctcgaagg agatcacgcc ttttttgctg aacagataag ctacggaacc
     1501 atcagtaccg aggttaccgc cacatttgct gaatgcatga cgcacttcag caacggtacg
     1561 gttgcggttg tcagacagac attcaatcat gattgccgtg ccgccaggac cgtaaccttc
     1621 gtagatgatg gtttccatgt ttgcatcatc atcaccgccc acacctcgtg caattgcgcg
     1681 gttcagagtg tcacgggtca tgttgttaga cagtgcttta tcaattgctg cacgcagacg
     1741 cggattagcg tccggatcac caccgcccag cttagccgcg gttaccagct cacgaatgat
     1801 tttagtgaag attttaccgc gcttagcatc ctgcgcagct ttacgatgtc tggtgttggc
     1861 ccatttacta tgacctgcca taaaaatatc tccagatagc cctgcctgtt caggcagcgt
     1921 taattacaaa ctgttcaatc gcctgccggt tgctccagga cttagtgagc gccgccgcag
     1981 caggcgcatc aagccacttg taagccagat gttcagtgaa aacgatctgg cgctcgtgcg
     2041 ggagcgcaag acagaaccat gattccgtat tacgcgtcac gcccggcgca tagcgatgac
     2101 gtaaatgtga aaaaatttca aactctaccg tgcgctgaca gtcaattaag gtcagttgtt
     2161 cagcgacaac atcaatggtg acctcttcct ttacttcgcg catggcagct tgcggcgcgg
     2221 tttcaccctc ttccacgctg ccggttaccg actgccagaa atcgggatcg tcacgccgct
     2281 gcaacatcag cacccgtttc gtatcttgtg cgtagatgac cactaagatc gaaacgggac
     2341 gcttataagc catatcagtt attctcagcc ttcttcacaa cctgaatgct cagctcagcc
     2401 agtgcagtcg ggttagcaaa gctcggtgct tcagtcatca gacacgccgc cgccgtggtt
     2461 ttcgggaagg cgataacgtc acggatattg tcggtgccgg tcagcagcat ggtcagacgg
     2521 tcaagaccga atgccagacc tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg
     2581 aagccgaatt tctcgcgctg ttcctcttcg ttgataccca gaataccaaa caccgtctgc
     2641 tgcatatcac cattatggat acgcacagaa ccaccgccca cttcgtaacc attgatgacc
     2701 atatcgtaag cgttagccac cgcattttcc ggtgcagctt tcagttctgc tgccgtcatg
     2761 tctttcggtg aggtgaacgg atggtgcatt gctgtcaggc cgccttcacc gtcgtcttca
     2821 aacatcggga agtcgataac ccacagcggt gcccatttgc tttcgtcggt cagaccaagg
     2881 tctttaccca ctttcaggcg cagtgagccc atcgcgtcgg caacaatttt cttgttgtcg
     2941 gcaccgaaga aaatcatatc gccatcttgc gcgccagtac gctccaggat ggcttcgatg
     3001 atttctgcat taaggaactt cgctaccggg ctgttgatac cttccagacc tttcgcgcgt
     3061 tcgttaactt tgatgtaagc cagacctttc gcgccgtaga ttttaacgaa gttaccgtat
     3121 tcgtcgatct gcttacgggt caacgatgcg ccgcccggaa cacgcagagc ggcaacacgg
     3181 cctttcggat cgttcgccgg acctgcaaat actgcaaact caacagattt cagcagatcg
     3241 gcaacgtcgg tcagttccat cgggttacgt agatctggtt tatcagaacc ataacggcgt
     3301 tctgcttctg caaaggtcat taccgggaaa tcgcccagat ccacgccctt cacttccagc
     3361 cacagatgac gcaccagcgc ttccatcact tcacgcactt gcggcgcggt catgaaagaa
     3421 gtttccacat cgatctgagt aaattcaggc tgacggtcag cacgcaggtc ttcgtcacgg
     3481 aagcatttaa cgatctgata gtagcggtca aagccggaca tcatcagcag ctgtttgaac
     3541 aactgtgggg attgcggcag tgcgtagaat ttacctttgt gcacacgaga aggcaccagg
     3601 tagtcacgcg cgccttccgg cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag
     3661 ccgtggtcat ccataaaacg gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc
     3721 tgagccattt ccgggcgacg caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg
     3781 ttgacgtggt tagagtcaag cggcagaaca tctgcacggt tgatgatagt cagcgaggac
     3841 gccagtactt cgatttcgcc agtcgccata tcgcggttaa tatttttttc gtcacgcgca
     3901 cgtacggtgc ccgtgacctg aatgcagaac tcattacgca gttcagaggc cagctttaac
     3961 gcgtccgcac gatccggatc gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg
     4021 atgaagatca ggctaccaag atcacgacga cggttgaccc aaccacacag agtcacctgc
     4081 tgccccacgt gggacaaacg gagctgtcca caatattctg tacgcatgag atatccctta
     4141 acttagctgc cggcggatgc cccctgctgc gcaggtgacc aagtcgcagc gttagctgta
     4201 tgtcacaact gaatgaaaaa aggcggctat tatactggaa attctgccgc accgtaagag
     4261 cctggcccgc gctggaacgc ctcgttacca ctttatatcg ggcctgaaat cagactctac
     4321 gccagtttgc tataaaggtg ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg
     4381 ccatcggctc atcgtattaa ggtgagaggc acgattttgt tttatcagga gtcatcatgc
     4441 ttgaacttaa tgctaaaacc accgcgctgg tggtgattga tttacaagaa ggcatcttgc
     4501 cttttgccgg tggtccacat actgccgatg aggtggttaa tcgcgccggg aagctggcgg
     4561 cgaaatttcg cgcgagcggt cagcccgtgt ttctggtgcg tgttggctgg tctgccgatt
     4621 atgctgaagc attaaaacag ccggttgatg ccccttcccc cgctaaagtg ttgcccgaaa
     4681 attggtggca acatcctgta gcattaggtg caaccgacag cgatatcgaa atcatcaaac
     4741 gtcaatgggg tgcgttttac ggtacggatc tggaattgca gttgcgccgc cggggtatcg
     4801 atacaatagt gttatgtggg atctcgacca atatcggtgt tgaatccacc gcccgcaatg
     4861 cctgggaact cggttttaat ctggtgattg ccgaagatgc ctgtagcgcc gctagcgccg
     4921 agcagcacaa taacagcatt aatcatatct acccgcgcat cgcccgtgtg cgtagcgttg
     4981 aagagatcct ccacgcgtta tgatttacat cggtttgcca caatggtcgc atcctaaatg
     5041 ggtgcggttg gggatcacca gccttgaaga gtatgcccgc cactttaact gtgtggaggg
     5101 taacaccacg ctttatgccc tgccgaaacc cgaggttgtc ctgcgctggc gtgagcagac
     5161 cacagatgac ttccgcttct gttttaagtt tccggcgact atttcgcatc aggcagcatt
     5221 acggcattgc gatgatttag tgactgaatt tttgacccgc atgtcaccgt tggctccgcg
     5281 cattggacaa tactggctgc aactgcctgc cacattcggc ccacgggagc tgcctgcgct
     5341 ttggcatttt ctcgattctc ttcccggcga atttaattat ggtgtggaag tccgccatcc
     5401 acagtttttc gccaaagggg aagaggaaca aacgcttaat cgcggtttac atcagcgcgg
     5461 cgttaatcgg gtgattttag acagccgccc ggttcatgca gcacgtccac acagtgaagc
     5521 tattcgctac gctcaacgaa aaaaacctaa agttccggta catgctgtac tgacggcgaa
     5581 aaatccactg atccgtttta tcggtagtga tgatatgacg caaaaccggg aattatttca
     5641 ggtctggtta caaaaattag cgcagtggca tcagaccact acgccttatc tttttttaca
     5701 tacgccagat attgcccagg ccccggaact ggtacatacc ctgtgggaag acttacgtaa
     5761 aacgcttcca gagatcggag cagttccggc tattccacag caatcttctc ttttctgaat
     5821 ttgccaccta tcatagacag gtgccatcgg ccattttaaa gggagtttgt atggtaagcg
     5881 cgctgtatgc cgttttaagt gcgttgttat taatgaagtt ctcttttgat gtcgttcgcc
     5941 tgcgaatgca gtaccgcgtt gcctatggcg acggcggttt tagcgaactg caaagcgcta
     6001 ttcgcattca tggtaacgcg gtggaatata ttcccatcgc gattgtgttg atgctgttta
     6061 tggaaatgaa tggcgcagaa acctggatgg tgcatatttg cggcatcgtt ttgcttgctg
     6121 gtcgtctgat gcattattac ggttttcatc accgtctgtt ccgctggcga cgttctggca
     6181 tgagcgccac ctggtgtgcg ctgttgctga tggtgctggc gaatctttgg tatatgccct
     6241 gggagttggt tttctccctg cgttagcgca caatacgcca ctttcttttt cccggatttt
     6301 tacgttatgt ctcaccgcga cacgctattt tctgccccta tcgccagact gggcgactgg
     6361 acctttgatg aacgggtagc tgaagtcttc ccggatatga tccagcgttc cgttcccggc
     6421 tattccaata ttatttccat gattggtatg ttagccgagc gcttcgttca acctggtacg
     6481 caggtttacg atctgggttg ttctctgggc gcggcgacac tctcggtgcg tcgcaacatt
     6541 catcatgata attgcaaaat tattgccatc gataactccc cggcgatgat tgaacgctgc
     6601 cgtcgtcata ttgacgccta taaagctcct acgccagtag acgttattga aggtgatatt
     6661 cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga attttaccct gcaattcctg
     6721 gaaccttccg agcgccaggc gttactggat aaaatttatc aaggactgaa cccgggcggc
     6781 gcgctggtgc tttcggaaaa attcagtttc gaagatgcca aagttggtga actgctgttc
     6841 aacatgcacc acgactttaa acgtgccaac ggttacagcg aactggagat cagccagaaa
     6901 cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg aaacccataa agcacgcctg
     6961 cataaagccg gttttgagca tagcgagctg tggttccagt gctttaactt tggttcactg
     7021 gtggcattaa aagcagagga cgctgcatga tcgactttgg taacttttat tctctgattg
     7081 ccaaaaatca tctttcacac tggctcgaaa cgctgcccac gcagattgct aactggcagc
     7141 gcgagcagca gcacgggctg tttaagcagt ggtccaacgc ggtggaattt ctgcctgaaa
     7201 ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa gagccactga
     7261 gcgccgggca aattaagcgc attgaaacgc tgatgcgcaa cctgatgccg tggcgcaaag
     7321 ggccgttctc actgtatggc gtcaacatcg ataccgaatg gcgttccgac tggaaatggg
     7381 atcgcgttct gccccatctt tctgatttaa ccgggcgcac cattcttgat gtcggctgcg
     7441 gcagcggtta tcacatgtgg cgcatgattg gcgctggcgc gcacctcgcg gtggggatcg
     7501 accctacaca actgtttctg tgccagtttg aagcggtgcg taaactactg ggtaacgatc
     7561 agcgcgcaca tttgttaccg ttaggtattg aacaacttcc ggcactgaaa gcctttgata
     7621 ccgtcttttc gatgggcgtg ctttatcatc gtcgttcacc gctggagcat ctctggcagt
     7681 taaaagacca actggtgaat gaaggcgaac tggtgctgga aacgctggtt attgatggcg
     7741 atgagaacac ggtgctggtg ccgggcgatc gttacgcaca aatgcgtaat gtctatttca
     7801 ttccttccgc gctggcgctg aaaaactggc tgaagaagtg tggttttgtt gatattcgca
     7861 ttgcggatgt gagcgttacc accacagaag agcagcgacg caccgaatgg atggtcaccg
     7921 agtctctggc cgattttctc gacccgcatg atccgggtaa aacggtggaa ggttatcctg
     7981 cgcctaaacg cgcggtgctg attgcgcgca agccgtaaaa atttagcgcc cgtttccggg
     8041 cgctttttac gtcttatgcc tgaatagctg gctgatcaaa cgccgttaac ttcagcgcat
     8101 tgcccgtata tttttcgata tacaccagcg cagagttacc ggcacaggca ttcgccagct
     8161 gcgagctggg gatatccgcc gttaacacgt tcgcactacc gtttttacac aagccatttt
     8221 ccagatctgg ccatgcacct tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca
     8281 ccaccgcgcc tgtcagaatc tgaccacgtt tgttccagac acgcaccaga tcgccattcg
     8341 caataccaaa gcgagcagca tcttcggtgt gaatagtgat tggttcacga tcggcaaccg
     8401 catatttttt acgcagttcc gcatagttaa gctgactgtg taaacggtgt gccggatgtg
     8461 cggtcagaag ctgcaactgc ttctcgtcgg cggtaccctt ccactcatca ggcgcaagcc
     8521 aggttgggtg tgccgggcaa tccttatagc caaatttttc cagcgttctg gagtaaatct
     8581 caattttgcc gcttggcgta cccagcgcat ttttcaccgg atcggcgcgg aaatcaccat
     8641 aacgaatgta ctgttcgttc ttctcgctgc ggcgcatttc gatcagttta ttttgctgcc
     8701 agaaggcatt aaacatcggc atcgtcacgc gttgcgcacg ggcacctttc tgagcggcat
     8761 catagaaaaa tttcagccac gccatttcat ctttaccttc ggtatagatc tcttttccgc
     8821 caggtttgag taattcagca agatcggcaa acacgtcaaa atcgttgcgc gcttcaaatt
     8881 gcggagcgac agcctgcttc atcggcacaa tatgctggtt gctgtaatca ccggtcatcg
     8941 tcaggtcatt gcgctcaaac gatgtggtga tcggtaatac gatatctgcg tgtttggctg
     9001 ctgcggtcca gtagcattca gaaacgacga tcatctccgg tttctgccat gctttaatca
     9061 gacgattagt atcctggtga tgggtaaagt taccgccacc cgcccaccag atcattttga
     9121 tattcggata cgtttgttct ttaccgttat gctgatattt gccgccagga ttttccagcg
     9181 catcgacgat acgggcaaca ggaatcgccg tcattccgcc atcatcagcg gcctcgctgg
     9241 cctgtccggc aatcgccgca gacatttctg gtagcacgcc gccaacacgc gtcgggttgc
     9301 caccgttgga gtaatgataa gagaaaccaa acccgccgcc cggtgtacca atttgcccta
     9361 acatcgccgc cagcgtcacc agcatccagt gtttctgttc accgtattgc tggcgctgaa
     9421 ttccccagcc tgccatcagc attgtgcgat ttgccgccat cagttctgca agtttcacaa
     9481 tctgcgcttc cggcacgcca gtaatttccg ccgcccaggc cgcactcttc ggcgtattat
     9541 cgttcttgcc tgtcagatac tcttcaaact gcggataccc ggtagtgtat ttttcaagga
     9601 acactttatc gtgtttgcct tgtgtcatca gggtatgcgc aatccctaac atcagtgcca
     9661 cgtcggtgcc catattcggt gcgatccagg tggcattatc gccaaagaat tcgatggttt
     9721 cggagcggat aggatcaatg gcaatcactg gtttgccaga ttttttcagc tgatggaagt
     9781 attccagccc ttgttcatcg gtactgctcc aggcaatttt taaggtattc agcgggttca
     9841 tgccccacaa caccaccacc tgactgtttt ccagaatcag cggccaggaa gtctgctgtt
     9901 catagacctc cacagaaccg accacatgcg gcatgatcac ctgtgccgct ccggttgaat
     9961 aatcgccgct atgcccggaa taaccgcccg ccaggttcat ataacgttgc agtaaggttt
    10021 gcgctttatg caacacgcca gaagaacgcc agccgtaaga tccggcaaaa atggccgatg
    10081 gtccgttagc tttacgaata tgatcgtgtt gctcatgaat cagttttaat gcttgttccc
    10141 agctcacctg tacataggta tcttcgccac gacctttcgc cggttgcagt ggattatcga
    10201 gatagctttt tctcaccatc ggatgctgaa tacgcgccgt ggtgtgtacc tgatccgccg
    10261 ccgtagactg taaggagttc ggtatggtct tcgccagcgc gcctgtcgaa gaaacaatct
    10321 tgccgtcctt cacttctacg ttcatcgctc cccaacgtcc cgcggtgaga attttgccgc
    10381 ccttctcttc tgcccatgca ggtaacggtg cagccgatgt caccaccagc gccccagcgg
    10441 caataccgct atgtttaata aattcgcgtc ttgttaatgt cataacttcc tccctgatca
    10501 acgaggatca ctgtttctcg gtaatatctt tggcgttgta ctggaagtac cgcgtcaaaa
    10561 tgtccagttc gttttcgctc atgctggttc gcgcacccat tcctttggca atggacggcc
    10621 atgcattgac ggtgtaatgg tcggcggcaa taggggcatg gcagccagcg caataggtat
    10681 cgtcaagttt ttcagcgtat tgccatagtg gtttacggtc cgctaatgcg gaatcggtta
    10741 gcgcaccctg taaagacgcc tgacgccatt gattgccgta ttcgtcagcc tgccattccc
    10801 cgtttaccgt tagcgccttg ataccttctt cacttaatgt ggcgagcgcc agccgttgac
    10861 ctgccgccag gtagagcgtg ttttcactgc cctgcatttg ataaccctgc aaccgaacaa
    10921 ttggctgttt accactgaca tcaacgacgg tgagatcggt accaggagtc acggtagcca
    10981 ggtcgcctat gcgagaagtt ttgaaaggat aaatatgtgc gccattagta actgaagtag
    11041 cggcctgact ttccagctca tgcgccgcgt tgtcatccat ttttatttct ggcgggaaat
    11101 gggcaatgcc tttatggcaa tcgatacaag tttcgccgtc cttttgcgct ttattatgca
    11161 ttttctgcgc agattcactt tgcgaggaaa tatccatggc atcaaaagaa tggcaactac
    11221 ggcaggttgc agagtcagtt gcttttaatt ctttccatac tgtttcggcc atttcctggc
    11281 gatgagcttc gaacttatcg tcactgtcta ttttgccgct aacaaattca tgataaatat
    11341 ctttagatgc ttttaattta gcaaataaat aatccatccc tgactttgga atatggcaat
    11401 cggcacattc cgcgcgtata cctttttggt tcgaaaagtg gacagttccc tgatattcct
    11461 caaaaggttt actcatcgag tggcaggaaa ggcaaaatgc agtatccgac gttttatgta
    11521 aggctttttg cgccagcaat aacccgccgc caccaaccac aaccgctatc agcaaaaaca
    11581 ataacccaat gcgttttttc cctcgcatag attcctctaa ttaaattcca ggacaccgat
    11641 accattgtat aaataaatat acaacgaatt gactacttgc ttaattcggc acaacactgc
    11701 ctgaaataat cgataaagaa tatgatttat tac
//
LOCUS       DACADA010000138        11522 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.140, whole genome shotgun sequence.
ACCESSION   DACADA010000138 DACADA010000000
VERSION     DACADA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.140"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..155)
                     /locus_tag="ISB17_003709"
     CDS             complement(<1..155)
                     /locus_tag="ISB17_003709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981598.1"
                     /translation="MSELPFNEVNGVWKQLAAGYASTQGESFTGHGGSPVAVEEAEHF
                     FVHFDQKG"
     gene            154..606
                     /gene="radC"
                     /locus_tag="ISB17_003710"
     CDS             154..606
                     /gene="radC"
                     /locus_tag="ISB17_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP1981599.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNHNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            653..1582
                     /locus_tag="ISB17_003711"
     CDS             653..1582
                     /locus_tag="ISB17_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1981600.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(1569..2183)
                     /locus_tag="ISB17_003712"
     CDS             complement(1569..2183)
                     /locus_tag="ISB17_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981601.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGEPNGIYRPEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWARE
                     DVAWMLQPENINAQINIQNQKYARLSAEDETETESGNASTAKNI"
     gene            2406..2603
                     /locus_tag="ISB17_003713"
     CDS             2406..2603
                     /locus_tag="ISB17_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP1981602.1"
                     /translation="MNTNRILRKKEVLHLTGNSSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            2605..3072
                     /locus_tag="ISB17_003714"
     CDS             2605..3072
                     /locus_tag="ISB17_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981603.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            3160..4014
                     /locus_tag="ISB17_003715"
     CDS             3160..4014
                     /locus_tag="ISB17_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981604.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
     gene            4591..5988
                     /locus_tag="ISB17_003716"
     CDS             4591..5988
                     /locus_tag="ISB17_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981605.1"
                     /translation="MSTEKDYPVKIPKWVYDRITEITDYVVGDTQWAVTRRQTVRWFL
                     AHIWLETDDDGWTICTVRDIRSSYASLLGQCEISYQGECFMSTLTDFLPTLSDIEFRA
                     GKASKDPEKRRASAWRFNPLLPVCDESARGKLNLVDLDTGESVSFRALLQGNGKAPGH
                     AIDVEKRQTALKQTEKDFLAKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGR
                     IEGQYVTYDHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFR
                     QYDVDYRVKSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYGWCG
                     NSKKKALKILRWWNRYISPLKSALESLLERVHGAHRKSCSSPRNYHRYANEVGLILDL
                     NSEVYQREKTHHHQYARNKALLAFMICGVEQAYIREVVSMNPGRVCMLDHDGVVATGA
                     LSLPDWRGFTMKVKD"
     gene            6166..6790
                     /locus_tag="ISB17_003717"
                     /pseudo
     CDS             6166..6790
                     /locus_tag="ISB17_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6985..9642
                     /locus_tag="ISB17_003718"
     CDS             6985..9642
                     /locus_tag="ISB17_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HAP1981606.1"
                     /translation="MDELLTLGCIALFFALVVVPILAIIALSRSTAVRSELATLRRRV
                     EVLEQRGVTEAPATVVTTVPQSVSQMVVPVETSSPAVNIVLSEPVADVTPESVDPWRP
                     QPKPQNEALPTPAVEQSSAFGGVMSSLVRWFMQGNPLAKLGILLLFLGFSFLLRYTVE
                     HSLFPLELRLVATSLFAIALLVVGWRLRHKQLVYALIIQGGATGTLYLTVFGAFWLWQ
                     MLPMTLAFALLVAICAASVGLAIMQKALSLAMLASIGGYLAPLLLSTGGGSHVALFSF
                     YLLLSVGILAISIWQHWRELNLLGLLFTFGIGGLWGLDGYRSEYYLSSQLFLIANTII
                     FGVLSVALSLRAQEKGKQIIDGVLLFASPLVGFGMQYAITRHMEYGPALSALGYGGFY
                     LALAWLALRRYPSLGRPLVLAALALGGAFTTLAIPLALSARWTAMAWALEGLGILWLG
                     VQQQQRRMSYSGTALLVLAVCSALWAQMNGMSALSLVLIFAVLSLSWLAAAWLWRNIQ
                     LQGSWVLLAGGLIFWIIALIGASQLVLKKDSLVLSGVLALMAISVWGWRIVSGRLAWW
                     ELDVSKWLLWPTMLVMLLSQISQHEIFAAGWQNLAWCLALPAAGALLWRDAETLPPRL
                     SRLAHLSLFWMILLALAAELFWFAQDLPWGMAAWGSGLMMAAGGLLIFLVHEAVHRQL
                     WPFRSWPALYASQAMIPVAAVLVVLLVLTNLQDGVVYRQTWLPLVNPLEEGAAFALLG
                     LIVFYRVSQRFFPVQISVCRPWPLIALLALSFWWLNGILLRALAWYGEVPWSAETLWH
                     SRLIQTCFALFWMLAALVVMLRATRRCSRREWLCGSVLLGIVIVKLMLVDSARGGGLA
                     RAVTFIGVAILVLIVGYFSPLPPKAGEEK"
     gene            9639..11034
                     /locus_tag="ISB17_003719"
                     /pseudo
     CDS             9639..11034
                     /locus_tag="ISB17_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
BASE COUNT         2651 a         2726 c         3231 g         2914 t
ORIGIN      
        1 cccttctggt caaaatggac gaagaaatgc tctgcttctt cgacagcaac gggcgaacca
       61 ccatgccccg taaagctttc tccctgcgta ctggcgtaac ccgccgccag ctgtttccag
      121 acgccattta cttcattgaa cgggagttct gacatgacca tgtttaccca gcgtgaaacc
      181 cgtatccttg accaggcgcg tgacattatt agccggtact accagcgtgg ggttcaactc
      241 tgttcccctg atgatgttcg acggtgcgtg atggttgagc tggcaccgct tgaacatgaa
      301 gagtttggca tcatcctact cgataatcat aatcagctgc tgcaccgcga aatcctgttc
      361 aggggtacgc taaactcggt cagcgttcac ccccgtgaag ttattaagcg tgtgctgaaa
      421 cataacgccg ccgccgtcat ccttgtccat aatcacccca gcggcgagcc tgaacccagc
      481 cggtgtgaca tccagttgac caaaaagctt caggagctgc tggaaatact ggatgtgcgg
      541 ttacttgatc actttatcgt ggcaggaact gaaacggtgt cgatggctga gcgaggactg
      601 gtgtgatgct gaaagttact tggtttaatg tacgttattg cgggttacgc gcatgagcag
      661 gacagcgttg cgtcaggtcg tgccggtggt gcaaaccacc gtgttcgatg cgtatattgt
      721 cggtctggcc ccctcgggac ggcgaggtat caccagcctg ttaaaccgca gcgccagcat
      781 acttaaacac ggtgcggatg cagcagacta tccgtgggaa caacttaact acgccgccgt
      841 tgccaaagtg cgcgcggcac tgctggatga cggttacgcc gtgtcatcgg tcaacatggc
      901 gctgtcagca ctccggggcg tagcgcagac ggcctttaac ctcaattaca tggatgccga
      961 aacgctggca cgtatccggt cagttaagcg cgtgagcggc gatgtccagc gcaagggaag
     1021 ggcgctgggc aggcaggaaa tccgggtact gattcaggct gcaaaacagc atccgcaatc
     1081 tgtacggcgc tgtcgcgatg tcgccattgt gctgatgctt tgcggaacgg gcctgcgggc
     1141 cggggaactg gttaaacttg agcgccggga ctatgacaac ggcatcctga cggtgaggca
     1201 agggaagggg cgtaaatacc gtgaaattca cgtggcggat gcggtggaca aggctattcg
     1261 tgcgtggctg aaagttggcg cagacgaggc tgacagtgcg ttgtttaggc gaattcagcg
     1321 cagtggtaag gtcgcaagcc aaccgctgac tacgaccggg ttaacgggta tcctggctga
     1381 gttacagcaa acggcaggta tagcacgttt taccccgcat gatatgcggc gcaccttcat
     1441 cacccgtttg ctggagcagg gcgtggatat caataccgtc cgccagttgg caggccacag
     1501 cgacatatcc acgacgacgc gctatgactg tcgtggtgat actatgaaga tcagtgccag
     1561 caagcgcatc agatgttttt agcagttgag gcgttgccag actcggtttc agtttcatct
     1621 tcagcactca accgcgcgta cttttgattc tggatgttaa tttgtgcatt gatattttca
     1681 ggctgaagca tccacgcaac atcctcacgc gcccattctt taccgtcaga gagtcgtttt
     1741 aaccggggtt tccatattcc atactcatta agtttttctg ctaccgaagc atatttctca
     1801 cgatctgact cccccgtatg aagcttgtca aaacgacctt ttacaagctg gtaaacagta
     1861 tagatttcca gtgccttagg ggacctggcc gggactatca tggggtgttc ctccggccta
     1921 taaatcccgt ttggctctcc ctcattgagc aggtcattaa acatgtggtt cacgcaggca
     1981 ctcagcacat ctgccgcttt ctccgcagcg aatttatccg ggctaaaacc cgctcccgtc
     2041 agtttccggg caatcagtgc cagtttttgc cgggtatccc gtcccagata aagcttcttc
     2101 tccagaaatt caaagacctc gttactgctt ttccgttccc ggtggcatct ggcgtcagcc
     2161 ttgttgcttt ggttaccaat catagtcacc tccatgacag tgttttgtac aaaatatcac
     2221 cattgagcct cagtgaaaat tgcttcttta tatgaccgtt ctggagacag aaatagatga
     2281 tctcgaatga catccacatt aaagatgccc ttaacgtgct caacgaaggc atcgttgagt
     2341 gaatccttgc gttttttggt ggttgtaaat aacccgctcc tgaaacagct caaaggagca
     2401 caagcatgaa caccaaccgt attttgcgta aaaaagaggt acttcatctg acggggaact
     2461 cgtcagcgac gttgtaccga ctcatctcga aaggattctt tcctttgtca aagaggctaa
     2521 cgggcgacaa tggccgggcg gtaggctggc tggagagtga tatcaataac tgggttaaca
     2581 gccgtatgca ggcgggagaa tgaaatgaaa gagataaaat cgaaaaccag ttctgcgaaa
     2641 aaaacgctgg ttccttggtt cgaaactatg gggaaacagg ttggtttatc acaagaaacg
     2701 atatcagcag tgttaaagga gcagcgttta cggatacgta agcttaacta cgcatctgca
     2761 tatacagtgt ccaatattct catacaggat tgcggcgggg ccgatgacat gtggagcaac
     2821 tacgctaaac tggcgcttat ccttgagcgt tgtaaataca ttccctgcgg gagcagggcc
     2881 tgtgcatatt gttctaccgc tcagcaatta cattatcagg gggtggatgt aaccggggaa
     2941 tacgctttat atcagttgcc tgagttattt ttagattttt tagaaacaaa cgacaaaaat
     3001 tcctcttatc attgggatta caataagtta gttgagcggc atgaagatgc tcaggcgagg
     3061 tcattcgggt aatagccatt tctgtatggt gaaaaactga catattaccc tgccactgca
     3121 ttttggtggc agggtaatca atacagctag gaatattaaa tgaacgattc ttattacttc
     3181 agaacatctg acccggatta cattcgtcac gcaccgtcaa tgcttgagat tttaagtaaa
     3241 gcattcccca ctattattac ccagcaggaa agtgagtatg aactgcagcg tcttaaccat
     3301 ttactaaata aactgaaagg cagcaatcgc tgctacaaca tcattgctca aaaactaagg
     3361 cagtgcagaa acggatctcc atgcaacagt cttatatgcc cgcattgcca gcgggaacgc
     3421 attctggcgc aactggctat gttgcatgtc ttgccgggga attcagcaga atacgtggga
     3481 gtggtcctgt ttttcaacaa ggatacgcaa actccgccgc catggaagaa cattggggcg
     3541 ctcagggcgc aaatcggtcg ttataagcag aggataagcc gggtacttaa ccgcctgggg
     3601 tacgccggtc cggccacagg caccttcagt atgatgcgac atatgcctga tggcccagaa
     3661 gagcgtattt tctgggttcc acagttgtgt ttgttcctgc ccaatgacag tacgttgatt
     3721 aaggggctaa aagcgcatat gtcgcgaagt ggtggggcat tcattgatgc atcgacgctt
     3781 aacacaccag tgatcgtgct caggtataaa gatcccgcga gactgatttc atgcgccctg
     3841 aatccagtct ggcacacagc agattacaca ctgactgata aagacgctct tgttaagtca
     3901 agaatggagc tactgaaagg ccggacactg ttaaaaagtc tgcttaccct ggactcactc
     3961 ggaacaggtg tcgtttcttt ttcgtttggg caacccccag ggaaagtgca ctgatttacc
     4021 ggtaatgcag caactgcaat gcacttttgc tgttgattaa attgcagtgg aacgacgaat
     4081 aacagcggca accactgcat tttcgttgtt gtaaaactgc aaagcttgcc agtgattttg
     4141 atggcgctga tgcactttaa cattccaact gtctcgattt taacgcaacc gtctgttttc
     4201 tgcaggggct cacggatgcc tctgcacacc cgatccttac catcaactga cgagttagcg
     4261 catctgtgag ggctttctgt gtttctgtca gtgaatattc tacacacaat ggggggagcg
     4321 gtcagcgagc accattgcca ttgagcgcaa cggagagctt ggggacatgc agcactacgg
     4381 aggcttcatc tccttctcat gtcattaaac gatacggcga acgagcgcag cgagtgaggg
     4441 aaggcgtcag cctgaacagg gggaaagatt tgcgaccgga gtggagcaaa tcatgggcgg
     4501 agcccatcct cgcgactggg gttttgactt tcgaattgcc cagcgttgag tttggttaat
     4561 gtagtgagac gaggatgctg agaggggacc gtgagtactg aaaaggatta tccagtgaag
     4621 atccctaagt gggtttatga ccgtattact gaaataacgg attatgtcgt gggggatacg
     4681 caatgggctg ttacccggcg gcagacagta cggtggttcc tggctcacat atggctggaa
     4741 acagacgatg atggctggac tatatgcact gtccgggata tccgtagcag ctatgccagt
     4801 ctcctgggac agtgtgaaat tagttaccag ggggagtgct ttatgtccac gctaactgat
     4861 ttcctgccga cactttcgga tattgaattt cgtgcaggaa aagcgagcaa agacccggaa
     4921 aagcgtagag ccagtgcatg gcggttcaac cccctgctgc ctgtgtgtga tgagtctgcc
     4981 aggggtaagt taaatctggt tgacttggat actggtgagt ctgttagttt tagagctctg
     5041 cttcagggga acgggaaagc tcctgggcat gcgatagatg tagagaaacg ccagacggcc
     5101 ctaaaacaaa cggaaaagga cttcttggcc aaagttgggc gggggcgcat gagtatacag
     5161 tttgtgaagg aattgcgtag ccgtgagccg gatcactatt accgcgcagg aatacgcagc
     5221 cttaaccatc tctacaatgg caggattgag ggacaatatg tcacttacga ccatcattat
     5281 cgtctgacgt ttggagggcg gtattatgat caagctttcc agaatctgcc caatgagttc
     5341 aaagctaaat ttcggaccgg gttactcaac tatgacatcg aagcgtgcaa tctggcctgt
     5401 ctgaaccatc tgttcagaca gtatgacgtc gattatcgtg tgaagtcatc gatatataaa
     5461 accatgatgg aacataccgg gctgacccgt aaacagtgca aacagatggt ccataccacc
     5521 acctacagaa ttgggcgtgt gactattggt gttaatgatg gccttggggc aaaagtgtat
     5581 ggatggtgcg gtaacagcaa aaaaaaggcg ctaaagattt tgcgttggtg gaaccgttat
     5641 atcagtccat tgaaatctgc gcttgagtca ttgctggaac gtgtgcatgg cgcacaccgc
     5701 aagagttgtt catcaccgcg taactatcac aggtacgcta atgaagtcgg attaatactg
     5761 gatcttaata gtgaggtgta tcagcgtgaa aagactcacc atcatcaata tgcgcggaat
     5821 aaggcactgt tggcgtttat gatctgtggt gtggagcagg cgtatatccg tgaggttgtc
     5881 agtatgaacc ctggtcgtgt ctgcatgctg gatcatgatg gggtagtggc aacgggcgcg
     5941 ctttcactac cggactggcg tggtttcacg atgaaagtga aggactagct tactttcttg
     6001 aatttaacta tctgcgatct ctgataaggg cgtattacgt tgtcaatcat aatgggatat
     6061 gcgcattgga acgatgtgca tatccagtaa gtgatccatg tccgctttga gctgtgagtt
     6121 caacgggtcg atgcaacgct gtccctaatc aagggggacg ttgccatgaa acgaagaact
     6181 cgtattaact acacgccaga gcaaaaggtg attatttggg acagatataa gcaaggtgat
     6241 tccctgcatg atatccccca gaatgttcga cagatttcac tctactatca tgcctacaat
     6301 ccaccaggcc ggtggctacc gtcctcccgt gcgaaagcgg caccggttag cgttttcgct
     6361 tgatgaaaaa gaggagatat ccagaggact ggtagcaaaa cgcatcatca gggacatcgc
     6421 tgctaaatta tcaatagctc cctcaacgat tagccgcgag atcaggaggc acggcggtgc
     6481 aaaacaatac cgtgcagcaa aagccgatcc tgctgcgtgg gaaaatgctc tgagaccaaa
     6541 accttgcaag ctaattgaaa gccccacatt gtgtaaaatc attgcagaga agatgcatca
     6601 ggactggtcg ccggaacaaa ttggcggttg gctaaaacgc tgttatcccg ataatcagga
     6661 aatgcatgtg tcacatgaaa tgatttataa aacgcttttt ataccaaccc ggggagcttt
     6721 aaaaaaagag ctgcagcaat gcctcagaag cggaagagta gtgcgtagat ccaaaacctc
     6781 atcacttaaa gtaaacagag atacattctg gtcacgaatg cccggttgtt cgtgctttgc
     6841 gttttaatcg cattttacag tttctgcctg cgtgcagagg ttgatggtat tttctgaaaa
     6901 agcggctcgc tttgctaatg catatggcac aaatacaaca gtttgtgtca ttctgaccca
     6961 tctttatacc gcgacggtgg aaacatggat gagctgctaa cccttggctg cattgcttta
     7021 ttcttcgcac tggtggtggt tcccatcctc gcgattatcg cgctcagccg cagcacggct
     7081 gttcgttctg agctggcgac cctgcgtcgc cgggtggagg tgcttgagca gcgcggtgtg
     7141 actgaagcac ccgccacagt ggtaacaact gtgccgcaga gtgtttcgca gatggttgtg
     7201 cctgttgaaa cgtcttcgcc tgcggttaac atcgtgctgt cggagcctgt ggctgatgtc
     7261 acgcctgagt ccgttgaccc ctggcgtcca caaccgaagc cgcaaaacga ggcgctaccc
     7321 acaccagcgg tcgaacaatc ttccgctttt ggtggggtga tgtcgtccct ggtgcgctgg
     7381 tttatgcagg gcaacccgct ggcaaagctg ggtattctgc ttctcttcct tggcttctcc
     7441 ttcctgctgc gttatacggt tgaacactcc ctgttcccgc ttgagctacg tctggtggcg
     7501 acttcgctgt ttgcgattgc cctgctggta gtgggctggc gattgcggca taaacagctg
     7561 gtttatgcgc tgatcataca gggtggagct acggggacgc tctatctcac cgtttttggc
     7621 gcattctggc tctggcagat gctgccgatg acgctggcgt ttgcattact ggtggcgatt
     7681 tgcgcggcga gcgtgggact ggcgattatg caaaaagcgc tcagcctcgc gatgctggca
     7741 agtatcggtg gctatctggc accgctgttg ctttccaccg gcggcggtag tcatgtggcg
     7801 ctcttctctt tctaccttct gctttccgtc ggtattctcg ccatcagcat ctggcaacac
     7861 tggcgcgagc ttaatctgct tggcctgtta tttacctttg gtatcggcgg tctttgggga
     7921 ctcgacggct atcgatcaga gtattacctg agcagtcagc ttttcctgat agccaatacg
     7981 atcatttttg gtgtgctgag cgtggcgcta tcgctacgag cacaggagaa aggtaagcag
     8041 attattgatg gcgtgctgtt gtttgcatcg ccgctggtcg gttttggcat gcagtacgcc
     8101 ataacccggc atatggaata tggtccggcg cttagcgcgc tgggttacgg cggtttctat
     8161 ctggcgttag catggcttgc attgcgtcgc tatccctctt tggggaggcc gctggtgctg
     8221 gcggcgctgg cgctgggtgg ggcatttacc acgctcgcta tcccactggc gctttcggcc
     8281 cgctggacgg cgatggcctg ggcgctggaa ggtctcggca tcctctggct gggcgtgcaa
     8341 cagcagcagc ggcgtatgag ctatagcggc actgcgctgc tggtgctggc agtgtgcagc
     8401 gcgctgtggg cgcagatgaa cgggatgagc gcgctttctc tggtgctaat ctttgccgta
     8461 ctcagcctca gctggctggc tgccgcctgg ctgtggcgca atattcagtt gcagggtagt
     8521 tgggttctgt tggctggcgg tttgatcttc tggataatcg cgctcattgg ggcatcgcaa
     8581 ctggtgctga aaaaggactc gctggtgctg tctggcgtgc tggcgttaat ggcgatatcg
     8641 gtctggggct ggcggattgt ctctggtcgt ctggcctggt gggagctgga tgtcagcaaa
     8701 tggttgctgt ggccgacgat gctggtgatg ctgctgtctc aaatttcaca acatgagatt
     8761 tttgctgcgg gctggcagaa tctggcctgg tgtctggcgt tacccgctgc gggggcattg
     8821 ctctggcgtg atgctgagac gttgccgccg cgcctctcca gactggcgca cctttcactc
     8881 ttctggatga ttctgctcgc gctggcggcg gagctattct ggtttgcgca agatctgcca
     8941 tggggcatgg cggcgtgggg ctccggcctg atgatggccg cggggggcct gctcatcttt
     9001 ctcgttcatg aggcggttca tcgtcagcta tggccattcc gaagctggcc cgcactctac
     9061 gcttctcagg cgatgatacc ggtagctgcg gtattggttg ttctgctagt actgacgaat
     9121 ttgcaggatg gcgtagttta tcgccagacc tggctgccgc tggtgaatcc actggaggag
     9181 ggggccgctt ttgcgctact gggactcatt gtattttatc gcgtttcgca gcgctttttc
     9241 ccggtgcaga tttcggtatg ccgtccctgg ccgctcattg cactgctggc gcttagcttc
     9301 tggtggctga atggcatcct gctgcgcgca ttggcctggt acggcgaagt gccctggagc
     9361 gcggaaacac tgtggcattc gcggctgatt cagacctgtt ttgccctgtt ctggatgctg
     9421 gcggcattag tggtgatgct acgtgcgacc cggcgttgct cacgtcgcga atggctttgt
     9481 ggatccgtat tgttagggat tgttattgtg aaactgatgt tggtagacag tgcgcgtggc
     9541 ggcggcctgg cacgcgcggt cacgtttatc ggcgtggcaa ttctggtgct aatcgtcggg
     9601 tatttttcac cgttaccgcc caaagcggga gaagaaaaat gaaatggatg aaagcggtgt
     9661 tatgcagcgt gctactgagt actgcgggcg cggcggtcag cagcgatgaa gtgaaggaag
     9721 cgccaacgga ctacgcaaca ggcgtcgcgc tggaaaccac gggggcattc gccctggtat
     9781 cgcatttcgc tgccgcaggt ggtgtatcag agcactgcat ggccggattt gcgcgatgtg
     9841 cgggttttta accatctggg ggatatggtg ccgtttgcgc tggtggcgca gaaaacccag
     9901 accgttacgc cagagacagt ggctctgcgt cttttcccgc ttgatatgtc gccggttccg
     9961 ccgcgtgatg agggtcaacg tagtggggat gctgttgttt tacgttcaaa aaacggtatt
    10021 gaaattcatt tgcaaagcga tgatgtcact gcgctcgggc agagctattt actgacgttg
    10081 ccggaagaga ggaaagactc tctttcactg acgcaattgc gtctgaactg ggggacgccg
    10141 acgggcaact ggcaggggaa agcctcggtt tatgtcagcc gtgatttacg ctactggcga
    10201 cccgtgcagg aagatgcgcc gttaatggat ctgacgcggg atagcgatcg tctgaaaatg
    10261 gacgccatca gcaccaacct gacattgtca cttgaaggaa atcgctacct gctggtgatc
    10321 ctcaactcgc aaagcccggc actgacgtta aacagcgtga gcgctattgc tgacagtaat
    10381 gagccggagt ccgaacgtat tgtgattggc gctcgggcgg ataaggtgtc ggatgatgaa
    10441 gccgtctggc gatggacaca gccgcagccc ctgacatcgt tgcggatcga tctggaaaat
    10501 gaaggcgttt taccggtgga actggtctgg cgcagcggcg aaaaagaacc ctggcagtca
    10561 ctgacaaaaa cggtgttgta tcgcctggac gggaagcgtt cagaagatat tcgtcttcct
    10621 ggccagttag ttgaggctgt tcgaatacgt acaattaatg cgcgattgcc ggaggcattg
    10681 ccagcattga gcggtgcgcg cgacagttat caactggttt ttaacacgca aggcaaaggt
    10741 ccttacatgc tggcgtgggg aaaccgggcg gcgaaaaagg ccgatgttgg actcgatatg
    10801 ttgatccctg cgtcgctgcg taaaacgcag gagatagata atctgccgtg ggctataccg
    10861 caggagagcg taactctcgg cggtgaattg cgactaacgg cgacgtcggc agccgaacaa
    10921 caaagtcagt ggaaaacgct gctggtgtgg ggggcgttaa tacttggcgt tgccgttctg
    10981 gcgtttatgg catggcgaat ctggcgagaa gttaaaaagg acggtgcggc gtaagccata
    11041 aaaaagcaca gttgcatagt tcacggtgtt gataaactgt tacaacagag gcgtttaagc
    11101 tgaggcgggg aaataatcta tttgtgaata atatgttact tcactggggc gtgagtcatg
    11161 ggaatgattt tgatacctgc gttttttatt ttagccatcc tgattatcgc tatcttcatt
    11221 tcagttaaga atggccgtca cgtatccgtt gtatgccttg ttgccgtggg tgagattttc
    11281 gtattcccat tctattttac ggcccttaag cgtttccgga gcatcaaaac ttggtaaaga
    11341 tttaccatgt tgacccatta tgcggcccct ctggagacca ctttcaggta ttttctctac
    11401 tgatatgtac acatggagag atgtgattgc aaattcccat cagcattact accaaaagaa
    11461 tcagacagga agcagcatag tattaataag aatattttca aacataaagg atgattatga
    11521 gt
//
LOCUS       DACADA010000139        11472 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.141, whole genome shotgun sequence.
ACCESSION   DACADA010000139 DACADA010000000
VERSION     DACADA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.141"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            135..1223
                     /locus_tag="ISB17_003720"
     CDS             135..1223
                     /locus_tag="ISB17_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1981607.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1265..2197)
                     /locus_tag="ISB17_003721"
     CDS             complement(1265..2197)
                     /locus_tag="ISB17_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1981608.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHRES"
     gene            complement(2289..2786)
                     /locus_tag="ISB17_003722"
     CDS             complement(2289..2786)
                     /locus_tag="ISB17_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP1981609.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3044..3649
                     /locus_tag="ISB17_003723"
     CDS             3044..3649
                     /locus_tag="ISB17_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAP1981610.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            3689..4552
                     /locus_tag="ISB17_003724"
     CDS             3689..4552
                     /locus_tag="ISB17_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1981611.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4542..6089
                     /gene="fdrA"
                     /locus_tag="ISB17_003725"
     CDS             4542..6089
                     /gene="fdrA"
                     /locus_tag="ISB17_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1981612.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6089..7507
                     /locus_tag="ISB17_003726"
     CDS             6089..7507
                     /locus_tag="ISB17_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1981613.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7660..8610
                     /locus_tag="ISB17_003727"
     CDS             7660..8610
                     /locus_tag="ISB17_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HAP1981614.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQDGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8620..10002
                     /locus_tag="ISB17_003728"
     CDS             8620..10002
                     /locus_tag="ISB17_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP1981615.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10379..11428
                     /gene="yahK"
                     /locus_tag="ISB17_003729"
     CDS             10379..11428
                     /gene="yahK"
                     /locus_tag="ISB17_003729"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HAP1981616.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2902 a         2842 c         3113 g         2615 t
ORIGIN      
        1 ataatcttct tgatagtgat gtgggatgtt atacgtatgg catcgctaat gtttatggtt
       61 accccttatg tgtgctcagg aatccacagg taatcactca tactgaacag cgataaaaga
      121 taaaaggtgt gttcatgaat tcatgtgatt ttcgtgtttt tctgcaagag ttcggtacaa
      181 cggttcattt gtcattgcct ggtagcgtat ccgagaaaga acgactgcta ctcaagctgc
      241 tgatgcaggg aatgtctgta acagaaatat cacagtacag aaatcgcagt gcaaagacca
      301 tttcacatca aaagaaacag ctctttgaga aactggggat tcagagcgat attactttct
      361 ggcgggatat tttctttcag tacaatccgg agatcatatc cgccacgggt aataatagtc
      421 acaaatatat taatgataat cactatcacc atatcgtcac gcctgaagcc atcagtctgg
      481 cgttggaaaa ccacgagttt aaaccgtgga tccaaccggt tttctgcgcg cagactgggg
      541 tactgacggg ctgtgaagtg cttgtccgct gggaacatcc acaaacggga atgatcccac
      601 cggatcagtt tattcctctg gcggagtcat ccggtcttat tgtcataatg actcgccagc
      661 tgatgaaaca gactgcggat attctgatgc cggtaaaaca tttgctgccg gacaatttcc
      721 atattggtat caacgtctcg gcgggctgtt ttttggctgc gggatttgaa aaagagtgtc
      781 tgaacctggt taagaaatta ggtaacgata aaatcaagct ggttcttgag ttgactgaac
      841 gtaatcctat tccggtaacg ccagaagcca gagcgatatt tgacagcctt catcagcaca
      901 atattacttt tgcgctggat gactttggta cgggttatgc gacctatcgt tacttgcagg
      961 cgttcccggt cgattttatt aaaattgaca agtcgtttgt gcaaatggcg agtgtcgacg
     1021 aaatctccgg tcatattgtg gacaatattg tcgaactggc gcgtaagcct ggtttgagta
     1081 tcgtggcgga aggggtagaa acccaggagc aggcggattt aatgatcggc aaaggcgttc
     1141 actttttaca gggctatttg tactctccgc cagtaccggg taataaattt atctctgaat
     1201 gggtaatgaa agcaggtggt tgatgtaaac cgctattcac agcgcatcgg gaggttggca
     1261 gcgattaact ctcccgatgc agtaaatcct gatagatccc cgttaatatg ccatttggcg
     1321 caaattgttt tttaatccac tgcgtcacct gaccggtggc agagtgctgg gttgcgagta
     1381 acatgcgtga atcctggcgc gggttatgga tttgtcgggt aaccagcaag gattgcgcca
     1441 tcgcctcacg gaccatgtaa tccggcaaaa aaccaattcc ttcacctaaa atctggcact
     1501 gacatttggt gttgaaatcg ggcaccagaa tcgattcctg cccatgcagc aaccagccta
     1561 cttttttatt aatcgtatgc gcggtgtcct ccaccatgat attagggtag agacgcagct
     1621 ggctttcggc gatcggctcc ggcacaaagg ctaatggatg atccggagcg atggcaaaag
     1681 cccagcgaat cgcgccaatc tccgtgtaat caatgccgcc accgtccagc agcgtgtcgg
     1741 gtgcgccgat agcgatattt gcctgattat taataatcgc atcccagacg ccgttataca
     1801 cttcggtggt gacggtaatc tggcaggtag gaaactgctt tttcagcacc tgtaataagc
     1861 gtgcggtgtg tttgggcgta taaagcagct gattaataca aatgcgcaca cgcgcttcta
     1921 tgccctgcga aatagtatcg atactgcgtt taatggcgtg aaaatcattc agcaggtcgg
     1981 tagctttgcg gaaaaaataa cgcccggact ccgttaactc aatgctgcgg gtactgcgag
     2041 tgaacagcac cacatccagc cctgtttcca ttcgcttgat ggtgtagcta atggcggaag
     2101 tggttaaacc caactcttct gcggctttac tgaagctgcc aaaacgtgca gcggtagtaa
     2161 atgccaacag attctcttcg gtaaaaattg agttcattaa accattcccg ccattatcga
     2221 atatgaattt ctccagccat gactgtagag gtatcggtta aagatagata atcatttttg
     2281 aataactttt aatacccgtc gcgtttagtc gtcgcttcgc ttaatttttg tcctccatat
     2341 tctgaaatta tccccacgca gttacccgcc agaaaaccca gtaatgtcac cgtccagttc
     2401 atcccgctgg caaaaaatag cgtcatgccg agaaagcctc cgggaataaa agacaataaa
     2461 ccgaaacgtc cttgccagac cactaccgct gagaaaggca cggtagtaat cacgctcgcc
     2521 cagaacccag aaatcccact caccgaggcc agccaaccgg cagccagcgc ggcgaaaaat
     2581 gcccacacca tcccggaata attaaccgcc agacttctga taaaccccga tttaccgccg
     2641 cctgcggcat aaaaactgca aaaggcgacg aagccaatgg ttcctaataa ttcccatccc
     2701 tgagagagcc cgacatgtga agacactaac tgccaaagcc ctgcacaaat accgacggtg
     2761 acacccgttg ccgtgagtcc gttcatcttg ttcccctctg gttagacatg gacaaaagct
     2821 actcttacat gtgcagacta tagagaaata aaacctgatt gagtgaaagg gtatcgggtc
     2881 aaagaaacaa atattgcact accgcacact gcgaaaagat tgttgaacat cattcaacaa
     2941 aaaggcgaaa tacggcgaat taaatttaag cgggttcttt tatcagatta tcgttgctat
     3001 tctcaattca tcgaaaacag ccacaaaaac caggggaatg tgaatgacta taaaaaatct
     3061 acctgccgac tatttattgg ctgcgcaaca gggtgatatc gataaagtaa aaacctgcct
     3121 tgcgctgggt gtcgatatta atacctgcga tcgtcagggg aaaacggcaa ttacgctggc
     3181 aagtttatat cagcaatatg cttgcgttca ggcattaatt gatgccggag cggatattaa
     3241 taagcaagat catacctgtt taaatccttt tttaattagc tgtctgaacg atgatttaac
     3301 gctactacga attattttac cggctaaacc cgatcttaat tgcgtaaccc gttttggcgg
     3361 tgtcggcctg acgcctgcct gtgaaaaagg ccatttaagt attgtaaaag agcttttggc
     3421 aaatacggag attaacgtta accagactaa ccatgttggc tggacaccgt tgctggaagc
     3481 gattgtgctt aatgatggtg ggattaaaca gcaggcgatt gtccagttat tgctggaaca
     3541 cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccgctgaaac tggcgcggga
     3601 acggggcttt gaagagattg cgcagttgct gatcgccgca ggtgcataaa ccgggaggct
     3661 tgttatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg ctgattttct
     3721 ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa atatcgcact
     3781 cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg cgcctaacag
     3841 ttgtcggttg gcactcactc actttaatgg tctgttccgg catgatgata aggttcagtt
     3901 tgacgatcaa ggcattacga ttggtcaaca tcatcatata gagatgagtc attgccagcg
     3961 ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg cctggcaaca
     4021 gtggtacgac attattcatc agcacctggg ggaaaatgaa accctgttta attatcgcgg
     4081 cgataatccg ttttatcagg cgttaaataa agaactacat attaaacgac gggcagttat
     4141 tcaggccgta aacgataaac aaaatatcgc ctcagcagtc gccagtatga tggggttagg
     4201 gattggcctt acgccatcag ccgatgatta tctgaccgga ctggtactta ttttatttat
     4261 ttccggacat ccggcggaaa aatacaaaga ggaattttat ctcggcctgc aacgcggcag
     4321 aaataatacc acgttattaa gtgccatcac gctggaagcc gcattacaac aacgctgccg
     4381 ggaaaatatt catcgtttta ttcacaacat tatttatgac atccctggga acgcaactca
     4441 ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc tgtatggcat
     4501 ggccgatggt tgtgcgctga gtcaaaccta cggagggaat tatgtcagtt aaaatagtca
     4561 ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg cgtgcaaata
     4621 aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac aaaggcgtgt
     4681 tgaagaattt aggactgctg acgccggagc tggagcaggc gaaaaacggc gacctgatga
     4741 ttgtcatcaa tggtaaatcg ggtgtggaca acgagcagtt gctggcggag attgaagaac
     4801 tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacggcact attgccagcg
     4861 ccaaaaagca tatcccggaa agtaacctgg cagtgatttc ggtcaacggt ctgtttgccg
     4921 ctcgcgaagc gcgtcaggcg ctgcaaaatg acctcaacgt gatgctgttt tccgataatg
     4981 tctcagttga agatgaactg gcgctcaaac agttggccca tgaaaaaggg ctgctgatga
     5041 tggggccaga ctgcggcacg gcgattatca acggcgcggc gctctgcttt ggtaacgccg
     5101 tgcgtcgcgg caacatcggt attgttggcg catctggcac cggcagccag gaattgagcg
     5161 tccgtatcca tgaatttggt ggcggcgttt cacaactgat cggcaccggc gggcgcgacc
     5221 tgagcgagaa aatcggcgga ctgatgatgc tcgatgccat cagcatgctg gaaaacgatc
     5281 cgcaaactga aatcattgtg cttatctcca aaccgcctgc gcctgcggtg gcccgcaaag
     5341 tgctggaacg cgcacgcgcc tgccgcaagc cagtggtggc ctgcttcctc ggtcgtggcg
     5401 aaacgccagt ggatgagcag gggctacagt ttgctcgcgg cagcaaagag gcggcattga
     5461 aagcggtgat gctctccggc gtgaaacaag aacatctcga tctgcatacg cttgaccagc
     5521 cgttgattgc ggatgtgcgc gcgcgtctgc aaccgcagca gaaatacatt cgtggtctgt
     5581 tctgcggcgg tacgctgtgt gacgaaaccc tgttcgcggt gatggaaaaa catggcgatg
     5641 tctacagcaa tatccagccc gatcctgaat tccgcctgca agatatcaac cgcagcatca
     5701 aacacacctt cctcgacttt ggcgatgatg acttcaccaa cggcaagccg catccaatga
     5761 ttgacccgac caaccgtatc agccgcttga tcgaagaagc gcgcgatcca gaagtggcgg
     5821 tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc ggttccacca
     5881 tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag ttgatcattc
     5941 tcgcctatgt gctgggcacc gatctcgata cgccatcatt agaacaacaa agccagatgc
     6001 tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg ctggcgcgtg
     6061 aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc aaccgttgaa
     6121 cgttattaac gtcggtatcg ccatgtttag cgatgatctg aaaaagcagc atgtagaagt
     6181 gacccaactc gactggacgc cgccggggca gggcaatatg caggtggtgc aggcactgga
     6241 caacattgcc gattcgccgc tggcggacaa aatcaccgcc gctaaccagc aggcgctgga
     6301 gcgcattatc cagtcgcatc cagtgctgat tggttttgat caggcgatta acgtggtgcc
     6361 gggcatgacg gcgaaaacca ttcttcacgc cgggccgccg gtcacctggg aaaaaatgtg
     6421 cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttt gaaggactgg cgaaagatct
     6481 cgacgaggcg gctgagctgg cggcttccgg ggagatcacc ttctcgccgt gccacgagca
     6541 cgactgtgtg ggatcgatgg cgggcgtcac ctcggcctcg atgtttatgc acatcgtgaa
     6601 aaacaaaacc tatggcaaca tcgcttatac caacatgagc gagcagatgg cgaagatttt
     6661 gcgtatgggc gctaacgacc agagcgtgat cgaccgcctc aactggatgc gcgatgtgca
     6721 ggggccaatg ctgcgtgacg cgatgaaaat tatcggcgag atcgatctgc gcttaatgct
     6781 ggcgcaggcg ctgcatatgg gcgatgaatg ccataaccgc aataacgccg ggacgacgct
     6841 gctgattcag gcgctgacgc cggggattat tcaggcgggt tattccgttg agcaacagcg
     6901 cgaagtgttt gagtttgtcg ccagcagcga ttacttctcc ggcccaacgt ggatggcgat
     6961 gtgtaaagcg gcgatggatg cggcgcacgg cattgaatac agcaccgtgg tgaccaccat
     7021 ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc aatggtttac
     7081 cggcccggcg cagcaggtca tcggcccgat gtttgccggt tataagccgg aagattcggg
     7141 gctggatatc ggcgacagcg ccatcaccga aacctacggt atcggcggat ttgcgatggc
     7201 gaccgcgccc gcgatcgttg cgctggtggg cggcacggtg gaagaggcta tcgatttctc
     7261 ccgtcagatg cgtgaaatca cccttggcga aaaccccaac gtcaccattc cgctgctcgg
     7321 ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg gcattctgcc
     7381 ggtgatcaac accgccattg cccataaaga tgcgggcgtc ggcatgattg gcgcgggcat
     7441 tgtgcatcca cccttcgcct gcttcgagaa agccattctt ggctggtgcg aacgttacgg
     7501 cgtctgacct gcaaagtacg cgttgtctct gccggatgcg gcgtgaacgc cttatccggc
     7561 ctacgagtgg cacgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     7621 ggtcataacc ccatcaccct ctgtcgcgga cataacaaca tgaaagaact tgtggtcgtt
     7681 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     7741 gaggcggtga aagccgtggc cgatacggtg ctggaaatgc tggcttccga ttacgacatt
     7801 gtgctgaccc acggcaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     7861 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcgga tacgcaaggt
     7921 ggcattggct atctgatcca gcaggcgctg aacaaccgac tggcgcgtca cggcgagaaa
     7981 aaagccgtca ctgtggtgac tcaggtggaa gtggataaaa acgatccggg ttttgcccat
     8041 cccaccaagc ccatcggcgc attctttagt gaaagccagc gtgacgaact acaaaaggca
     8101 aaccctgact ggcgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     8161 ccggaaccga aacgtattgt cgaagcacct gccattaagg cgctgatcca acaaggcttt
     8221 gtggtgattg gcgcgggcgg cggtggaatt cctgtggtgc gcaccgaagc gggagattac
     8281 caaagcgtgg acgcggttat cgacaaagat ctctccaccg cgctactggc ccgcgaaatt
     8341 cacgccgaca ttctggtgat caccaccggc gtcgaaaaag tctgtatcca ctttggcaag
     8401 ccgcagcaac aggcgctcga tcgcgtagat attgccacca tgacccgcta tatgcaggaa
     8461 gggcatttcc cacctggcag catgttgcca aaaatcatcg ccagcctgac gttcctggaa
     8521 caggacggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctacgcggc
     8581 gaaacgggca cccatattat taaaacctaa ggacgtaaga tgaaagaaaa caatagccgc
     8641 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctca
     8701 gtgccgctcg cccatgcggg ggtagctggc accctaaact gcgaagcgaa caacaccatg
     8761 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     8821 gaaaatggcg tggcggtaca gacccgcacg gcgcgccaga ccgtggagat tcaggacggt
     8881 aaaattgttg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     8941 gctggcggta agctgatgct gcccaccacc cgcgatatgc atattcatct cgacaaaacc
     9001 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     9061 atcaaactcg agcagaaaat gctgccggaa ctgcaaccgt acacgcagga acgggcggaa
     9121 aaactgatcg atttattgca gtcgaaaggc accactattg cccgcagcca ctgcaatatc
     9181 gaaccggttt ccggcctgaa aaatctgcaa aatttgcagg cggtgctggc gcgacgtcag
     9241 acgggcttcg agtgtgagat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     9301 gaatccttaa tgcgcgaagc gatgcaggcg ggggcgcatt acgtcggcgg gctggacccg
     9361 accagtgttg atggcgcgat ggaaaaatcc ctcgacacca tgttccagat tgcgctggac
     9421 tatgacaaag gcgtcgatat tcacctgcac gaaaccacgc cggcaggcgt ggcagccatc
     9481 aattatatgg ttgaaacagt ggagaaaacg ccacagctga agggcaagct gaccatcagc
     9541 cacgcctttg cgctggcaac gctcaacgaa caacaggtag atgaactggc gcaccggatg
     9601 gcggcgcagc gaatttcgat cgcttcgacg gtgccgattg gcacgctgca tatgccgctc
     9661 aaacagctgc acgacaaagg cgtaaaagtg atgaccggca ccgacagcgt tatcgaccac
     9721 tggtcgcctt acggcctggg cgacatgctg gaaaaagcca atctgtacgc gcagctctat
     9781 attcgtccta acgaacagaa cctctcccgc tctctgtttt tagccactgg cgatgtattg
     9841 ccgctcaacg aaaaaggcga gcgcgtgtgg cccaaagcgc aggatgatgc cagttttgtg
     9901 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tttcgccgag aacggcgacg
     9961 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatacgaact tgatatgttt
    10021 gcaacggtga acagtaagag atttaagccc cagggagtgg ggcggcctga gtatgagtgt
    10081 gtcggcagaa atggacatta tctttgaaga atatgccctt attgacgttg tgtagataac
    10141 tatttgtcta cgtgaaaata tcatcagttt tgccgatttt tgtcttttac ggatagcaaa
    10201 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggctc
    10261 gcggcatgtt agggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
    10321 tgccgcgatg acatatcagg catcgtcaaa tacacatagc taatcaggag taaacacaat
    10381 gaagatcaaa gctgttggtg catattccgc taaacaaccg cttgaaccga tggatatcac
    10441 ccggcgtgaa ccgggaccgc atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
    10501 ttccgatctc caccaggtcc gttcggagtg ggcggggacg gtttacccct gcgtgccggg
    10561 tcatgaaatt gtggggcgcg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
    10621 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgaaga
    10681 cgggctggaa aactactgtg atcacatgac cggaacctat aactcgccga caccggatga
    10741 accgggccat actctgggag gctactcaca acagatcgtc gttcatgagc gatatgttct
    10801 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttattgt gtgcaggaat
    10861 caccacgtac tcgccgctac gccactggca ggccgggccg ggtaaaaaag tgggcgtggt
    10921 cggcatcggc ggtctgggac atatggggat taaactggcc cacgcgatgg gggcgcatgt
    10981 ggtggcattt accacttctg agtcaaaacg cgaagcggca aaagccctgg gggccgatga
    11041 agttgttaac tcacgcaatg ccgatgagat ggtggctcat gtgaagagtt tcgatttcat
    11101 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgcga
    11161 tggcaccata acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
    11221 cctgatcatg aaacgccgcg cgatagctgg ttctatgatt ggcggcattc cagaaactca
    11281 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
    11341 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
    11401 tatcgataat cgcacactaa cagactgaaa aaaattaata aatatcctgt ggtttaacat
    11461 attaatttcg ct
//
LOCUS       DACADA010000140        11278 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.142, whole genome shotgun sequence.
ACCESSION   DACADA010000140 DACADA010000000
VERSION     DACADA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.142"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            233..1804
                     /gene="garD"
                     /locus_tag="ISB17_003730"
     CDS             233..1804
                     /gene="garD"
                     /locus_tag="ISB17_003730"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAP1981617.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1953..2288
                     /gene="prlF"
                     /locus_tag="ISB17_003731"
     CDS             1953..2288
                     /gene="prlF"
                     /locus_tag="ISB17_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HAP1981618.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2288..2752
                     /locus_tag="ISB17_003732"
     CDS             2288..2752
                     /locus_tag="ISB17_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HAP1981619.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2807..3616)
                     /gene="agaR"
                     /locus_tag="ISB17_003733"
     CDS             complement(2807..3616)
                     /gene="agaR"
                     /locus_tag="ISB17_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAP1981620.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3865..5145
                     /gene="kbaZ"
                     /locus_tag="ISB17_003734"
     CDS             3865..5145
                     /gene="kbaZ"
                     /locus_tag="ISB17_003734"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAP1981621.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5168..5641
                     /gene="agaV"
                     /locus_tag="ISB17_003735"
     CDS             5168..5641
                     /gene="agaV"
                     /locus_tag="ISB17_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP1981622.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5652..6431
                     /locus_tag="ISB17_003736"
     CDS             5652..6431
                     /locus_tag="ISB17_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAP1981623.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6421..7299
                     /locus_tag="ISB17_003737"
     CDS             6421..7299
                     /locus_tag="ISB17_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP1981624.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7317..7751
                     /locus_tag="ISB17_003738"
     CDS             7317..7751
                     /locus_tag="ISB17_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1981625.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7748..8881
                     /gene="nagA"
                     /locus_tag="ISB17_003739"
     CDS             7748..8881
                     /gene="nagA"
                     /locus_tag="ISB17_003739"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP1981626.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9231..10385
                     /locus_tag="ISB17_003740"
     CDS             9231..10385
                     /locus_tag="ISB17_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HAP1981627.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            10398..11258
                     /gene="kbaY"
                     /locus_tag="ISB17_003741"
     CDS             10398..11258
                     /gene="kbaY"
                     /locus_tag="ISB17_003741"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAP1981628.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2697 a         2910 c         2992 g         2679 t
ORIGIN      
        1 ttaattagta ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc
       61 acagatttgc gctttacctt gccgggaggt actgcaatcc ctgaaatgat tgacattgat
      121 cacatttctg catttaaact cctgacattc ttatttcacc caatgaagtt atttattttt
      181 aaatgagacc aggtcctcat tttaatagtc cctggctgga gaatattgca caatggccaa
      241 catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa
      301 tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct
      361 ggaattaatt gaacatattc cccaggggca taaagtcgca ttgctggaca ttccggctaa
      421 tggtgaaatt attcgttatg gcgaggtgat tggttacgcc gtgcgcgcaa tcccacgcgg
      481 aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac atacgctgcc
      541 actggcaacc aaagtcccgg aacccttacc gccgctggaa ggatacacct ttgagggcta
      601 tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca
      661 ctgtgtggca ggcgtggtgg actacgtagt aaaaatcatt gaacgcgatc tgctaccgaa
      721 atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggttgtg gcgtggcgat
      781 taacgcaccc gcggcagttg tacctattcg taccattcac aatatttcgc tgaaccctaa
      841 ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc ctgaacgcct
      901 actgactgga acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt
      961 gcaggatgaa aagcatgtcg gttttcagtc catggtcgag gatattttgc aggtagccga
     1021 acgccatcta caaaaactga atcaacgtca gcgagaaacc tgtccggctt cagaactggt
     1081 tgtcggcatg cagtgcggtg gcagcgatgc gttttctggc gtaacggcaa acccggcggt
     1141 tgggtatgcg tctgatctat tggtgcgctg cggcgcaacg gtgatgtttt cagaagtcac
     1201 agaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa
     1261 acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg
     1321 cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaacgtgg tagagaaggc
     1381 actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt cgcccggcca
     1441 acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg
     1501 cacgcaacag gtggcttcgg gtatcaccgt gcaagtgttt acgaccggtc gtggtacgcc
     1561 gtacggcctt atggcggtac ccgtcattaa aatggcgacc cgcaccgagc tggcgaaccg
     1621 ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag aaaccattga
     1681 agaagtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac
     1741 tttctcggat caatggggac tgcataacca gctggcggtg tttaacccgg caccggtgac
     1801 ctgatttctt ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt tttattgttt
     1861 aaagtccaca catccattaa taatgcattt gcattacctt agttcaagct tataatttga
     1921 gcagaaaaca ggctgtaaaa ggacagtaaa taatgcccgc taatgctcgc tctaacgctg
     1981 tactgaccac tgaatcaaag gtcactatac gcggacaaac aactatcccc gcgccagtgc
     2041 gtgaggcctt aaaactgaag ccaggcctgg acagcattca ttacgaaatt ctgcctggtg
     2101 ggcaagtatt tatgtgccga ctgggagatg aacaggagga tcatactatg aatgcatttt
     2161 tgcgttttct ggatgcagat atccagaaca atccgcaaaa aactcgtcca ttcgacattc
     2221 aacaaggaaa gaaacttgtc gctggcatgg acgtcaacat tgacgatgag attggcgacg
     2281 acgaataatg gattttccac aaagggttaa tggttgggcg ctatatgctc atccctgttt
     2341 tcaggaaacc tacgatgctt tagttgccga agtcgaggca ttaaagggaa aagatcctga
     2401 aaattatcag agaaaagctg ccacaaagtt attggcggtg gtccataaag tgattgagga
     2461 gcatatcacg gtcaatccat catcaccggc attccgtcat ggcaagtcgt taggctctgg
     2521 gaaaaataaa gactggtcac gggtaaaatt tggtgctggt cgctatcgtc tcttctttcg
     2581 ttacagtgaa aaagagaaag tcatcattct gggatggatg aacgatgaaa acacgctgcg
     2641 cacctacggt aaaaaaacag atgcctatac cgtattcagt aaaatgttaa aaagaggaca
     2701 tcctcctgcc gactgggaaa gcctcaccca agagacagaa gaaaaccatt gatggtgttc
     2761 acaatgcccg ccgaacgcat tggtgttgac cagcgggcat cctttctcat tccccgacca
     2821 gaatcacttc aaccccagcc tttcgcagtc cttccagact atccgcagga atgccttcat
     2881 caacaatgat catgtcgata cgttgagtat caatgatctt atgtaaactg gaacggttga
     2941 acttactgga atcggtgacc acgatgatcc gttccgcaac ttcgcacatc cgacggttta
     3001 aacgggcttc atcttcatta tgcgtgctga cgccacgctc cagatcgatc gcatctacac
     3061 caagaaacag catatcgaag tggtaatttt gcagcgattg ctcagcctga tcaccgtaaa
     3121 aagattgcga ctgacggcgc aaatgccccc cggtcatcag cagctcaacg ccttccgctt
     3181 ccagcaacgc attagccacg ttcataccgt tggtcatcgc aattacgtcg gtgtgcttgc
     3241 gcatcagacg agcaatctca aaagtggtgg tcccggagtc gaggatcacc cgatgaccag
     3301 gctgaatcaa ctcaacggca gctttcgcaa cgctgcgttt catcgcggtg ttcagtgcgc
     3361 ttttatcttc cactgatggc tcgactgacg gcgttgtgct atcgcagatc aacgcgccac
     3421 cataggcacg cacggcgatc ccctgctttt ccagaaacgc cagatcgttg cggatcgtca
     3481 cagtagatac gccatacaat gccgacagat cgttaacctg cacactccct tgctgtcgca
     3541 gacgctgaat gatctgttct cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag
     3601 cgtcggtatt actcatagta agtcctttcg taaaactttc gtttcatttc gttttgccta
     3661 ttaacgcctt tctattaagc aaatgcaagc ccaccttacc cattggcgca agctactctc
     3721 gtttcagtgg ctttcattat gtttcttttg tgaatcagat cagaaaacta ttatctttcg
     3781 ttttattttt atctcaccat gacgcagtat caactgaaac aaaacgaaag attaatgtcg
     3841 cagtaatctg aactggagag gaaagtgaaa catctgacag aaatggtgag acagcacaaa
     3901 gcgggcaaaa caaatgggat ttatgccgtt tgttccgcac atccgctggt gctggaagct
     3961 gcaatccgct acgccagtgc aaaccaaacg ccgttactga ttgaagctac ctccaatcag
     4021 gtggaccagt ttggcggtta taccggaatg acgcccgccg gttttcgcgg ctttgtttgt
     4081 cagctcgccg actcgttgaa tttcccgcag gatgcgctga ttctgggtgg cgaccatctg
     4141 gggccaaacc gctggcaaaa tctgcctgcc gctcaggcaa tggccaatgc cgatgatttg
     4201 attaaaagct acgtggcggc aggattcaaa aaaattcacc ttgattgcag catgtcctgt
     4261 caggacgatc cgattccctt aaccgatggc atcgtggccg aacgcgccgc tcgtttggcg
     4321 aaagtggcgg aagaaacctg ccgtgaacat tttggcgaag ccgatctgga gtatgtcatt
     4381 ggtaccgaag tgccggtgcc tggcggcgcg catgaaacct taagcgagct ggcggtcacc
     4441 acgccggatg ccgcccgcgc cacgctggaa gcccatcgtc acgcttttga aaagcagggt
     4501 ctgaatgcca tctggccacg catcattgcc ctggtggttc aacccggcgt cgaattcgat
     4561 cacaccaacg ttattgatta tcagcccgcc aaagcgaccg ccttaagcca gatggtcgaa
     4621 aactacgaaa cgctgatttt cgaagcgcac tctaccgatt atcaaacgcc gcaatcgctg
     4681 cgccagctgg tgattgacca ctttgccatt ctgaaagttg gcccggcgct gacctttgct
     4741 ctgcgtgaag ctctgttctc tctggcagcg attgaagaag aactagtgcc agcaaaagcc
     4801 tgttctggtc tgcgtcaggt gctggaaaac gtgatgctcg accgcccgga atactggcaa
     4861 agccactacc acggtgacgg caacgcgcgt cgtctggcgc gtggttatag ctactcggat
     4921 cgcgtgcgct attactggcc ggacagccag attgatgacg ctttcgctca tctggttcgt
     4981 aatctggcgg attcaccaat tccgctgccg ctgatcagtc agtatctgcc gctgcagtac
     5041 gtgaaagttc gctccggcga gctgcagcca acgccacggg aactcattat caaccatatt
     5101 caggacatcc tggcgcagta ccacacagcc tgtgaaggcc aataagcaaa acaaagagga
     5161 acacgctatg ccaaatattg ttttaagtcg gattgatgaa cgcttgattc acggtcaggt
     5221 cggcgttcag tgggtaggat ttgcgggggc aaatctggtg ctggtagcca acgatgaggt
     5281 tgccgaagat ccggtacaac aaaacctgat ggaaatggta ctggcagaag ggatcgccgt
     5341 acgtttctgg acgctgcaaa aagtcatcga caacatccat cgcgccgccg atcgacagaa
     5401 aatcctgctg gtttgtaaaa caccggccga tttcctgacg ctggtgaaag gtggcgttcc
     5461 ggtgaatcgc attaacgttg gcaatatgca ctacgccaat ggcaagcaac aaatcgccaa
     5521 aacggtttct gtggatgcgg gtgatatcgc agcatttaac gacctgaaag ccgctggggt
     5581 ggaatgcttc gttcagggcg tcccgacaga gcctgctgtg gacctcttta aattactttg
     5641 agggattcat catggaaatc agtctgttgc aggcatttgc gttgggcatt atcgccttta
     5701 tcgctggcct ggatatgttt aacggcttaa cccatatgca ccgcccggtg gttctcggcc
     5761 cgttggtcgg gctggtactt ggcgatctgc ataccggtat tttaaccggc ggtacgctgg
     5821 aactggtgtg gatggggctg gccccgctgg cgggcgcaca gccgcctaac gtgattatcg
     5881 gtactatcgt cggcacggcg tttgccatta ctactggcgt gaaacccgat gtcgcggtcg
     5941 gtgtcgccgt acctttcgct gttgcagtac agatggggat taccttcctg ttctcggtga
     6001 tgtccggcgt gatgtcccgc tgcgaccgga tggcggagaa cgccgatact cgcggcattg
     6061 aacgcgtgaa ctatctggca ctgctggcac tcggcacttt ctattttctc tgcgctttcc
     6121 tgcctatcta cttcggcgcg gaacatgcaa aaaccatcat tgatgtcctg ccgcaacgat
     6181 taattgatgg cctcggcgtc gcgggcggca tcatgccagc aatcggtttt gccgtgctgc
     6241 tgaaaatcat gatgaaaaac gtctacatcc cctacttcat cctgggcttt gttgccgcag
     6301 cctggctcaa gttaccggtg ctggctattg ctgccgctgc gctggcgatg gcgctgatcg
     6361 acctgctgcg taaatctcct gaaccgactc aacctgcggc acagaaagag gaatttgaag
     6421 atggcatcta atcaaaccac cctgccgaac gtcagcgaaa atgaagaaac actgctgact
     6481 ggcgtcaatg aaaacgtgta tgaagatcag agcattggcg cagagctgac gaaaaaagat
     6541 atcaaccgcg tcgcctggcg ctccatgctg ttacaagcgt ctttcaacta cgaacgtatg
     6601 caggcttccg gctggttgta cggtctgctg cctgctctga aaaagatcca cactaataaa
     6661 cgggacctgg cgcgcgccat gaaggggcat atgggtttct tcaataccca tccgtttctg
     6721 gtgacgtttg ttatcggcat tatccttgcg atggagcgtt ctaagcagga cgttaacagt
     6781 attcagagca ccaaaattgc cgtcggtgcg ccgctcggcg ggattggcga tgcgatgttc
     6841 tggctaacgc tactgccgat ttgtggcggg ataggcgcaa gcctcgcgct acaaggctct
     6901 attcttggcg ctgtcgtctt tattgtgctg ttcaacgtgg tgcacctggg gctgcgtttt
     6961 ggtctggcgc attatgctta ccgcatgggc gtggcggcta ttccactcat taaagcaaat
     7021 accaaaaaag tcggccatgc ggcgtctatc gttgggatga cggtgatcgg cgcgctggtg
     7081 gcaacctatg ttcgtttaag caccacgctg gaaatcacgg cgggcgacgc agtggttaag
     7141 ttacaggctg atgttatcga caaactgatg cctgccttct taccgctggt ctacaccctg
     7201 accatgttct ggctggtacg ccgcggctgg agtccgctgc gcctgattgc agtgacagtg
     7261 gttctcggca tcgtcggtaa attctgccat ttcctttaaa tacaatgagg tattagatgt
     7321 taagtattat tttgacaggg catggcggat ttgccagcgg catggaaaaa gcaatgaagc
     7381 aaatcctcgg cgagcaatcg cagtttatcg ccatcgattt tccagaaacc tcaagcaccg
     7441 cgctgcttac atcgcagctt gaagaggcca tcgcccaact ggattgtgaa gacggcattg
     7501 tttttctgac ggatttgctg ggcggcacgc cgtttcgcgt agcttcaacg ctggcgatgc
     7561 aaaaaccggg ctgtgaagtg atcaccggca caaatttgca actattactg gaaatggtgc
     7621 tggaacgcga agggttaagc ggtgaagagt tccgtgtgca ggcgctggag tgtggacacc
     7681 gtgggctaac cagtctggtg gacgaacttg gtcgctgcca tgaggaatgt ccggtcgagg
     7741 aaggaatatg acacacgttc tgcgcgccag aaggctgctg actgaagagg gatggctcga
     7801 tgaccatcag ttgcgtattg ctgacggtgt aatcgcagca atcgaaccga ttccagtggg
     7861 cgtgactgaa cgcgatgcgg aactgctctg ccccgcttac atcgacaccc atgtacacgg
     7921 tggtgcgggc gttgatgtaa tggatgacgc gccggatgcg ctcgacaagc tggcaatgca
     7981 caaggcacgc gaaggtgtcg gcagttggtt accgaccacc gtaaccgcgc cgcttaatac
     8041 cattcatgcg gcgctgaaac gtattgctct acgctgccag cgcggtgggc ctggtgcgca
     8101 agtgctgggg agttatctcg aaggaccgta cttcacgccg cagaataaag gcgcacatcc
     8161 gccggagctg tttcgcgagc tggaaattgc cgagctggat caattgattg ccgtttctca
     8221 gcagacctta cgcgtggtag cgctggcacc agaaaaagaa ggggcattgc aggccatccg
     8281 ccatcttaaa cagcaaaacg tacgggtgat gctggggcat agcgcggcga cctggcaaca
     8341 aacccgtgcc gcgtttgatg ctggtgccga cggcctggtg cattgctata acgggatgac
     8401 aggtttacat caccgcgaac cgggaatggt tggcgcggga ttaacggaca agcgcgcctg
     8461 gctggaactg atagccgatg gtcatcatgt acatccggcg gcaatgtcgc tctgttgttg
     8521 ctgtgcgaaa gagaggatcg tactgatcac cgacgcgatg caggcagccg ggatgccgga
     8581 tggtcactat acgttatgtg gtgaagaagt gcagatgcac ggtggcattg tccgtaccgc
     8641 gttcggtggg ctggcgggca gtacgctgtc tgtcgatgcg gcagtgcgca acatggtcga
     8701 gttgacgggc gtaacgcctg cggaagccat ccatatggcg tcgctgcatc cggcgcgaat
     8761 gctgggtgtt gatggtgttc tgggatcgct taaaccgggc aaacgcgcca gcgtcgttgc
     8821 gctggatagc gggctgcatg tgcaacaaat ctggattcag ggtcaattag cttcgttttg
     8881 atggtttgct cctttattag gccttcactt cccccgtaag gcctttcttt ttctttcttt
     8941 ttgatctgtg cagcggtgtc ggatgcgacg ctaatgcgcc ttatccgacc tacggttggt
     9001 gaccgtaagg ccggataaag tatttacgcc gcatccagca atccctttta cttcccttgt
     9061 cttttctttc aacgatcaca gatttcgttt tatttccttt ttctccattg aactttcagt
     9121 ttcttttcta tagattttaa ccaacgaaag aaatcaccaa gtgaaatgaa acgaaaggca
     9181 agcgaaagcg acaacgccga cgtcaagttc atcagactaa ggattgagtt atgccagaaa
     9241 attacacccc tgctgctgcc gcaaccggta catggactga agaagagatc cgccatcagc
     9301 ctcgcgcatg gatccgttca ctcaccaaca ttgacgcgct acgttccgcg ctcaataact
     9361 tccttgaacc gttactgcgc aaagagaatc tgcaaatcat cctgaccgga gccggaacat
     9421 cggcatttat cggtgacatc atcgcgccgt ggctcgccag ccataccggt aaaaacttca
     9481 gcgccgtacc aaccaccgat ctggtcacca atccaatgga ctacctgaac ccagctcatc
     9541 cgctactgtt gatctccttc ggtcgctccg gcaacagccc ggaaagcgtc gccgccgtgg
     9601 aactggcaaa tcaatttgta ccagaatgct atcacctgcc gatcacctgc aacgaagcgg
     9661 gcgctcttta ccaaaacgcg atcaacagcg acaacgcgtt tgccctgctg atgcccgcag
     9721 aaacgcacga tcgcggcttt gcgatgacca gcagcattac caccatgatg gccagctgcc
     9781 tcgcggtttt cgcacctgag acgatcaaca gccaaacctt ccgcgacgtg gcggatcgtt
     9841 gccaggcgat cctgacctca ctgggcgatt tcagcgaagg tgtgtttggt tacgcaccgt
     9901 ggaaacggat cgtttatctc ggcagcggtg gcttacaggg cgcagcacgc gagtcggcgc
     9961 tgaaagtgct ggaactgaca gcgggtaaac tggcggcctt ctatgattcc ccgaccggat
    10021 tccgtcatgg cccgaaatcg ctggtcgatg acgaaacgct ggtagtggtg tttgtctcca
    10081 gtcaccctta cacccgtcag tacgatcttg acctgctggc agaactccgc cgtgacaacc
    10141 aggcaatgcg cgtgatcgcc atcaccgctg aaagcaacga cgttatcgcc gctggcccgc
    10201 atatcatcct gccgccgtcc cgtcacttta tcgacgttga acaagcattt tgcttcctga
    10261 tgtacgccca gacgtttgca ctgatgcagt cgctgcacat gggcaatacg ccggataccc
    10321 catcagccag tggcaccgta aaccgcgtgg tgcaaggcgt aactattcat ccgtggcagg
    10381 cataagagga tcgcattatg agcattatct ccaccaaata tctgttacag gacgcccagg
    10441 ccaatggcta cgcggtgcct gcctttaaca tccataacgc cgagacgatc caggcgatcc
    10501 tcgaagtgtg cagtgaaatg cgatcgccgg tgatcctcgc cgggacacca ggtaccttta
    10561 agcatatcgc gctggaagag atctacgccc tgtgtagcgc ctattccacg acctataaca
    10621 tgccgctggc actgcatctc gaccaccacg aatcgctgga tgatattcgc cgtaaagtcc
    10681 acgcaggcgt gcgcagcgcg atgatcgacg gcagccactt cccgtttgcc gagaacgtga
    10741 agctggtgaa atcggttgtc gacttctgcc actcccggga ttgcagcgtg gaagcagaac
    10801 tgggccgcct cggcggtgtt gaagatgaca tgagcgttga cgccgaaagt gcattcctga
    10861 ccgatccaca agaagctaaa cgctttgtcg aactgactgg cgtcgacagc ctggcggtag
    10921 cgattggtac ggcgcacggc ttatacagca aaacgccgaa gattgatttc cagcggctgg
    10981 cggaaattcg tgaagtggtg gatgttcctc tggtgctgca tggtgccagc gatgtcccgg
    11041 atgaatttgt ccgtcgcact attgaactcg gtgtaacaaa agtgaacgtt gccacagaat
    11101 taaaaatagc cttcgctggc gcggttaaag cctggtttgc ggaaaatccg cagggtaatg
    11161 atcctcgtta ttatatgcgc gtcggaatgg atgcgatgaa agaagtggtc agaaataaaa
    11221 ttaatgtctg tggttcagcg aatcgaattt cagcataatc attaatttta tacgtttt
//
LOCUS       DACADA010000141        11245 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.143, whole genome shotgun sequence.
ACCESSION   DACADA010000141 DACADA010000000
VERSION     DACADA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.143"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..294
                     /locus_tag="ISB17_003742"
     CDS             <1..294
                     /locus_tag="ISB17_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAP1981629.1"
                     /translation="AVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASA
                     ADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(414..593)
                     /locus_tag="ISB17_003743"
     CDS             complement(414..593)
                     /locus_tag="ISB17_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAP1981630.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            667..2028
                     /gene="pabB"
                     /locus_tag="ISB17_003744"
     CDS             667..2028
                     /gene="pabB"
                     /locus_tag="ISB17_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAP1981631.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSHLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLLEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2032..2610
                     /locus_tag="ISB17_003745"
     CDS             2032..2610
                     /locus_tag="ISB17_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAP1981632.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEGEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2794..4158
                     /gene="sdaA"
                     /locus_tag="ISB17_003746"
     CDS             2794..4158
                     /gene="sdaA"
                     /locus_tag="ISB17_003746"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP1981633.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4289..5887
                     /locus_tag="ISB17_003747"
     CDS             4289..5887
                     /locus_tag="ISB17_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1981634.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(5891..7447)
                     /gene="yoaE"
                     /locus_tag="ISB17_003748"
     CDS             complement(5891..7447)
                     /gene="yoaE"
                     /locus_tag="ISB17_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAP1981635.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7911..8882
                     /gene="manX"
                     /locus_tag="ISB17_003749"
     CDS             7911..8882
                     /gene="manX"
                     /locus_tag="ISB17_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP1981636.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8945..9745
                     /locus_tag="ISB17_003750"
     CDS             8945..9745
                     /locus_tag="ISB17_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP1981637.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9758..10609
                     /locus_tag="ISB17_003751"
     CDS             9758..10609
                     /locus_tag="ISB17_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAP1981638.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10664..11122
                     /locus_tag="ISB17_003752"
     CDS             10664..11122
                     /locus_tag="ISB17_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAP1981639.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2748 a         2841 c         2828 g         2828 t
ORIGIN      
        1 gctgtaatag ataaagccct ggatttcatt ggtgccatgg atgtatcagc gccaacacca
       61 agttcgatga atgaaagtac ggcgaaggga atctttaaat atttaaaaga actgggagta
      121 cccgcgagtg ccgctgatat tacggcgcga gctgaccagg aaggctggaa ccctggtttc
      181 acggaaaaaa tggttggatg ggcaaaaaaa atggagtctg gtgaacgctc tgtgattaaa
      241 aatcctgaat acttttcaac atatatgcag gaagaactaa aagcactggt ttgagttaaa
      301 ttcacatcag cataaatggg tcaggacgct tttaatcaca tataaaaagc gtcctttttc
      361 tcacaatcaa ataatcatcg gcttctataa cgaaagcgat acggattagt gtgttactcg
      421 tcttcatcct caaaacgcgc cacgattcgc tcgccggaat ggttggcacg cagctcttct
      481 gccaccagcg cgattgcctg gccgctgctc atcccttgtg ccatcagttc ctggatccgc
      541 tcgacggctt tttgctgctg ttcatgggtg agtgaaggta aacctgcaaa cattgttaac
      601 tcctgctaaa ttgttggcgc taattatttc atgctacccg gcacatagcc agtagagtca
      661 ggactgatga agacgttatc tcccgctgtg attactttac cctggcgtca ggacgccgct
      721 gaattttatt tctcccactt aagccacctg ccgtgggcga tgcttttaca ctccggctat
      781 gccgatcatc cgtatagccg ctttgatatt gtggtcgccg agccaatttg cactttaacc
      841 actttcggta aagaaaccgt tgttagtgaa agcgaaaaac gcacaacgac cactgatgac
      901 ccgctacagg tgctccagca ggtgctggat cgtgcagaca ttcgcccagc gcataacgaa
      961 gatttgccat ttcagggcgg cgcgctgggg ttgtttggct acgatctggg ccgccgtttt
     1021 gagtcactgc cagaaattgc ggaacaagat atcgttctgc cggatatggc agtgggtatc
     1081 tacgactggg cgctggttgt cgaccaccag cgtcaaacag tttctttgct gagtcataat
     1141 gatgtcaatg cccgtcgggc ctggctggaa agccagcaat tctccccgca ggaagatttc
     1201 acactcactt ccgactggca atccaatatg acccgcgaac agtacggcga aaaatttcgc
     1261 caggtacagg aatatctgca cagcggtgat tgctatcagg tgaatctcgc ccagcgtttt
     1321 catgcgacct attctggcga tgaatggcag gcattccttc agcttaatca ggccaaccgc
     1381 gcgccgttta gcgctttttt acgtcttgaa caggctgcta ttttaagcct ttcaccagag
     1441 cggtttattc tttgtgataa tggtgaaatc cagacccgcc cgattaaagg cacgctacca
     1501 cgcctgcccg atcttctgga agatagcaaa caagcagaaa aactggcgaa ctcaacgaaa
     1561 gatcgtgccg aaaatctgat gattgtcgat ttaatgcgta atgatatcgg tcgtgttgcc
     1621 gtagcaggtt cggtaaaagt accagagctg ttcgtggtgg aacccttccc tgccgtgcat
     1681 catctggtca gcaccataac ggcgcaacta ccagaacagt tacacgccag cgatctgctg
     1741 cgcgcggctt ttcctggtgg ctcaataacc ggggctccga aagtacgggc tatggaaatt
     1801 atcgacgaac tggaaccgca gcgacgcaat gcctggtgcg gcagcattgg ctatttgagc
     1861 ttttgcggca acatggacac cagcattact atccgcacgc tgactaccat taacggacaa
     1921 atttactgct ctgcgggcgg tggaattgtt gccgatagcc aggaagaagc ggaatatcag
     1981 gaaacttttg ataaagttaa taagatatta cgccaactgg agaagtaaga cgtggaatac
     2041 cgtagcctga cgcttgatga ttttttatcg cgctttcaac ttttgcgccc gcaaattaac
     2101 cgggaaacct taaatcatcg tcaggctgcc gtgttaatcc ccatcgtccg ccgaccgcaa
     2161 ccggggttgt tgctgactca gcgttcgatt catctgcgta aacacgctgg acaagtggca
     2221 ttccctggag gcgcagtcga tgacacggac gcatcagtta tcgccgctgc gctgcgcgaa
     2281 gctgaagaag aggtcgctat accgccttcc gccgttgagg ttatcggcgt gctgccgccc
     2341 gtcgatagcg tcactggcta ccaggtaacc ccagtggtcg gcattatccc gcccgatctg
     2401 ccgtatcgcg ccagtgaagg tgaagtctcc gccgtctttg aaatgccgct cgcccaggca
     2461 ttacatctgg gtcgttatca tcctttagat atctaccgcc gtggcgattc acatcgggta
     2521 tggctgtcct ggtacgaaca gtattttgta tggggaatga ccgcaggcat aattcgtgag
     2581 ctggcgctgc aaattggtgt gaaaccctga ctatacttat ctttacatct acaaaacact
     2641 acttgagaca atcatcgcaa tattagttaa atcgcggttt ttgattagtt taattcatgt
     2701 gaatagttaa gccagtcgcc gcgttccctc ttacactatg cgctgttatt agttcgttac
     2761 tggaagtcca gtcaccttgt caggagtatt atcgtgatta gtctattcga catgtttaag
     2821 gtggggattg gtccctcatc ttcccatacc gtagggccta tgaaggcggg taaacagttc
     2881 gtcgatgatc tggtcgaaaa aggcttactg gatagcgtta ctcgcgttgc cgtggacgtt
     2941 tatggttcac tgtcgctgac gggtaaaggc caccacaccg atatcgccat tattatgggt
     3001 cttgcaggta acgaacctgc caccgtggat atcgacagta ttcccggttt tattcgcgac
     3061 gtagaagagc gcgaacgtct gctgctggca cagggacggc atgaagtgga tttcccgcgc
     3121 gacaacggga tgcgttttca taacggtaac ctgccgctgc atgaaaacgg tatgcaaatc
     3181 cacgcctata acggcgatga agtcgtctac agcaaaactt attattccat cggcggcggt
     3241 tttatcgtcg atgaagaaca ctttggtcag gatgctgcca acgaagtgag cgtgccgtat
     3301 ccgttcaaat ctgccaccga actgctcgcg tactgtaatg aaaccggctt ttcgctgtct
     3361 ggtctcgcta tgcaaaacga actggcgctg cacagcaaga aagagatcga cgagtatttc
     3421 gcgcatgtct ggcaaaccat gcaggcatgt atcgatcgcg ggatgaatac cgaaggcgta
     3481 ctgccaggcc cgttgcgcgt gccacgtcgt gcgtcggccc tgcgccggat gctggtttcc
     3541 agcgataaac tgtctaacga tccgatgaat gtcattgact gggtaaacat gtttgcgctg
     3601 gcagttaacg aagaaaacgc cgccggtggc cgtgtggtaa ctgcgccaac taacggtgcc
     3661 tgcggtatcg ttccggcagt gctggcttac tatgaccact ttattgaatc agttagccca
     3721 gacatctata cccgttactt tatggcagcg ggcgcgattg gtgcactgta taaaatgaac
     3781 gcgtctattt ccggtgcgga agtcggttgc cagggcgaag taggtgttgc ctgttcaatg
     3841 gctgctgcgg gccttgccga gctgttgggc ggtagcccgg aacaggtttg tgtggcagcg
     3901 gaaattggca tggaacacaa cctcggtctg acctgcgacc ccgttgcagg gcaagttcag
     3961 gtgccgtgca ttgaacgtaa tgccattgcc tctgtgaagg cgattaacgc cgcacggatg
     4021 gctctgcgcc gcaccagtgc accgcgcgtc tcgctggata aggtcatcga aacgatgtac
     4081 gaaaccggta aggacatgaa cgctaaatac cgcgaaacct cacgcggtgg tctggcaatc
     4141 aaagtccagt gtgactaata cttcttattc gcccatctgc aacggatggg cgaatttatt
     4201 cccgctttct cgtctgctgt aattttcccc actacacttc cactgttgcg tcaggcgttt
     4261 gtcgccatac gcttacaggg tggcccgcat gcaaaaagca caacggatca ttaaaaccta
     4321 tcgccgtaat cgaatgattg tttgtacgat ttgcgcactc gttacgctcg cttcgaccct
     4381 gagcgtgcga tttatttcac agcgtaactt aaatcaacaa cgggtagtac aattcgccaa
     4441 tcacgctgta gaggaattag ataaagtact gcttccccta caggcaggta gcgaagtctt
     4501 gcttccgctg attggtctgc cctgctctgt cgcccatttg ccattacgta aacaggcggc
     4561 aaaactccaa actgtgcgat ccattggcct ggtgcaagac ggcacacttt attgctccag
     4621 catttttggt tatcgcaatg tgcccgtcgt ggacattctg gctgaacttc ctgcaccgca
     4681 accactttta cgactgacga ccgaccgtgc cctgataaaa ggcagtccgg ttttgattca
     4741 atggacgcct gcagcgggca gtagcaatgc tggggtcatg gagatgatta acatcgactt
     4801 actgacggca atgctccttg aaccacaact gccgcaaatc agtagcgcca gcctgacggt
     4861 ggacagtcgg catttgctct atggtaatgg gctggtagat tcccttccgc aacctgaaaa
     4921 caatgaaaac tatcaggttt cttcgcaacg ctttcctttt accattaacg ttaatggccc
     4981 gggggctacg gcgctggcat ggcactatct tccaacacaa ttaccgctgg cggtgctgct
     5041 aagtttactg gtgggctaca tcgcctggct ggcgaccgct taccgaatga gcttttcccg
     5101 cgagatcaat ctgggcctgg cgcaacatga gttcgaattg ttctgtcagc ctttgcttaa
     5161 tgcgcgcagc cagcaatgta ttggtgtaga gattttgctg cgctggaaca atccgcgtca
     5221 gggctggatt tcaccggatg tgtttattcc tatcgcggaa gaacatcatt taattgtgcc
     5281 actgacccgc tatgtgatgg cagaaaccat tcgtcagcgc catgttttcc caatgagtag
     5341 tcagtttcat gttggcatta acgtcgcacc cagccatttt cgccgtggtg tgctgataaa
     5401 agatctcaat cagtactggt ttagcgctca cccgattcag caactgatcc tcgaaatcac
     5461 cgaacgcgat gccttactgg atgttgatta ccggattgcc cgcgagctgc atcgtaaaaa
     5521 cgtcaaactg gcgattgatg acttcggcac cggcaacagt tcattttcct ggcttgaaac
     5581 attacgtcct gacgtgctga aaattgataa gtcatttacc gcagctatag gttctgacgc
     5641 ggttaactcg acggtgaccg atatcatcat cgcactgggg caaagactga atattgaact
     5701 ggtggcggag ggcgtagaaa cgcaagaaca ggcgaagtat ttgcgcagtc atggcgtgca
     5761 tattttgcaa gggtatttgt acgcacagcc gatgccgcta cgtgattttc ccaaatggct
     5821 ggcgggcagc caaccgccgc ccgcccggca taatggacat atcacgcccg ttatgccgtt
     5881 acgttaacgc ttactcatct tcatcgtgcg ccggttgctc tttaacaatg cgaaccagat
     5941 caacacgata atcattggct tcaatgatgg tgatatgcag tggacctaca tcaatcacat
     6001 cgcccacacg gggaatgtga ccatttgccg agatcacgag gcccgcgacc gtcgcgatat
     6061 catcgtcatc ggcaaggtgc tcaacatcaa gcgcctgctg caaggcatgc aaatctgtac
     6121 cgccttttac cagccagccg tcaccgtcgg taatgatttc cggcgtttcg tcagcgtccg
     6181 ggaattcacc cgcaatggct tccagcacat ccagcggcgt gaccagacct tgtaccacac
     6241 caaactcgtt ggtcacgata acaaagctcc cgcgagcacg acgcagcacg cccagcaggt
     6301 tgatcggatc gagggtttcc gggacgataa tcgccggaga cgccgaagct atcgccgcca
     6361 catcaactcc ctcttccagc gccaccagca gttctttagc acgtacgatg ccgataattt
     6421 catccagttc accgcgacat accgggaaca aactgtgcgg tgaggagagc agttgctcgc
     6481 ggatttcatc gaccccgaga ttagcatcaa cccagcttat ttcaccgcgc ggcgtcatga
     6541 tcccgcgcag agaacgcgac gccagcgtca gtacgccgtt aatcatgtaa cgttcttctt
     6601 cggcaaacgc accttccggg atcggcatcg gcatcgggtt atcggcatcg tgctgaacat
     6661 tggcctgacg tttcccgccc atcaaacgca ggatggcatc ggcagtacgc gctcgcagcg
     6721 gcaaagtcga ctggtgacga ataaagttgc gacgcgcaat ctggttaaac acttcgatga
     6781 tgatcgagaa gccaatcgcg gcatacaggt aacctttcgg aatgtggaaa ccgaaacctt
     6841 ctgccaccag actcagacca atcattaaca ggaagctcag acagagcacc accaccgtgg
     6901 ggtgctggtt aacaaatcgc gtcagcggtt tcgatgccag cagcataacc gccatcgcaa
     6961 tcactaccgc cgccatcatc accggcaaat ggttaaccat ccctactgca gtaattaccg
     7021 catccaacga aaagacggcg tcaaggatga caatctgtgt gacgaccacc cagaaactgg
     7081 cgtagccttt accgtggccg gaatcatgat cgcggttttc cagccgttca tgcagttcgg
     7141 ttgttgcttt gaacagcaag aatatgcccc cgaacaacat aatcaggtcg cgtccggaga
     7201 aggagaaatc catgacggta aatagcggtt tggtcagcgt gaccatccat gaaatcagcg
     7261 acagcagccc cagacgcata atcagcgcca gtgataatcc cagcaaacgc gctttatcgc
     7321 gttgttttgg cggcagtttg tcagcaagaa tggcgatgaa gaccaggtta tcgataccca
     7381 gcacaatttc gagaacaaca agcgtgagta gccccgccca aatcgagggg tccattaaga
     7441 attccatgac aagctcctgc ttaaggaata gctattcgac gccagaaata atgcaggcgt
     7501 aacgacaaaa tgcaaacgaa aggtgcggca taaagtgcca gaaagacagg cgttaaaagg
     7561 cctgatgctg aaatgacgtc ggtgacgatc catactgcgg gctactgccc tatactccat
     7621 ggttgttaaa caggagttaa acatatcaga gacgcctctg atttggcaaa gatttacctt
     7681 cctttgcaaa cgaatgtgac aaggatattt tacctttcga aatttctgct aatcgaaagt
     7741 taaattacgg atcttcatca cataaaataa ttttttcgat atctaaaata aatcgcgaaa
     7801 cgcagggggt ttttggttgt agcccttatc tgaatcgatt cgattgtgga cgacgattca
     7861 aaaatacatc tggcacgttg aggtgttaac gataataaag gaggtagcaa gtgaccattg
     7921 ctattgttat aggcacacat ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc
     7981 tgttaggcga gcaggaaaac gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa
     8041 cgctgattga aaagtacaac gctcagttgg caaaactcga caccactaaa ggcgtgctgt
     8101 ttctcgttga tacatgggga ggcagcccat tcaatgctgc cagccgcatt gtcgtcgaca
     8161 aagagcatta tgaagtcatt gcaggcgtta acattccaat gctcgtggaa acgttaatgg
     8221 cccgagatga tgaccctagc tttgatgaac tggtggcact ggcagtagaa acaggccgtg
     8281 aaggcgtgaa agcactgaaa gccaaaccga ttgaaaaagc cgcgccagca cccgctgccg
     8341 cagcacccaa agtggctcca actccggcaa aaccaatggg accgaacgac tacatggtta
     8401 ttggccttgc gcgtatcgac gaccgtctga ttcacggtca ggtcgccacc cgctggacca
     8461 aagaaaccaa tgtctcccgt attattgttg ttagtgatga agtggctgcg gataccgttc
     8521 gtaagacact gctcacccag gttgcacctc cgggcgtaac agcacacgta gttgatgttg
     8581 ccaaaatgat tcgcgtctac aacaacccga aatatgctgg cgaacgcgta atgctgttat
     8641 ttaccaaccc aacagatgta gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta
     8701 acgtcggtgg tatggcattc cgtcagggta aaacccaggt gaataacgcg gtttcggttg
     8761 atgaaaaaga tatcgaggcg ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc
     8821 gtaaggtttc caccgatccg aaactgaaaa tgatggatct gatcagcaaa atcgataagt
     8881 aacgtattgt gttgattatc actcagtttt cacacttaag tcttacgtaa acaggagaag
     8941 tacaatggag attaccactc ttcaaattgt gctggtattt atcgtagcct gtatcgcagg
     9001 tatgggatca atcctcgatg aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt
     9061 gggtatcgtt cttggcgata tgaaaaccgg tattattatc ggtggtacgc tggaaatgat
     9121 cgcgctgggc tggatgaaca tcggtgctgc cgttgcgcct gacgccgctc tggcttccat
     9181 catttctact attctggtta tcgcaggtca tcagagcatt ggtgcaggta tcgcactggc
     9241 aatccctctg gccgctgcgg gccaggtact gaccatcatc gttcgtacta tcaccgttgc
     9301 tttccagcac gctgcggata aggctgctga taacggcaac ctgacagcga tttcctggat
     9361 ccacgtttct tctctgttcc tgcaagcaat gcgtgtggct attccggccg tcatcgttgc
     9421 gctgtctgtt ggtaccagcg aagtccagaa catgctgaat gcgattccgg aagtggtgac
     9481 caatggtctg aatatcgccg gtggcatgat cgtggtggtt ggttatgcga tggttatcaa
     9541 catgatgcgt gctggctacc tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt
     9601 caccaacttt aacctggttg ctctgggtgt gattggtact gttatggcag tgctctacat
     9661 ccaacttagc ccgaaataca accgcgtagc cggtgcgcct gctcaggctg ctggtaacaa
     9721 cgatctcgat aacgaactgg actaacaggt gagcgaaatg gttgatacaa ctcaaactac
     9781 caccgagaaa aaactcactc aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt
     9841 tcagggttca tggaacttcg aacgtatgca ggcactgggt ttctgcttct ctatggtacc
     9901 ggcaattcgt cgcctctacc ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca
     9961 cctggagttc tttaacaccc agccattcgt ggctgcgccg attctcggcg taaccctggc
    10021 actggaagaa cagcgtgcta atggcgcaga gatcgatgac ggtgctatca acggtatcaa
    10081 agtcggtttg atggggccgc tggctggtgt aggcgacccg atcttctggg gaacggtacg
    10141 tccggtattt gcagcgctgg gggctggtat cgcgatgagc ggcagcctgt taggtccgct
    10201 gctgttcttc atcctgttta acctggtgcg tctggcaacc cgttactacg gcgtagcgta
    10261 tggttactcc aaaggtatcg atatcgttaa agatatgggt ggtggcttcc tgcaaaaact
    10321 gacggaaggg gcgtctatcc tcggcctgtt tgtcatgggg gcattggtta ataagtggac
    10381 acatgtcaac atcccgctgg tggtctctcg cattactgac cagacgggca aagaacacgt
    10441 taccactgtc cagactattc tggaccagtt aatgccaggc ctggtaccac tgctgctgac
    10501 ctttgcttgt atgtggctgt tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt
    10561 cttcgtcatc ggtatcgctg gttacgcttg cggcctgctg ggactgtaag actgttgtac
    10621 actaccgggg ccttttggcc ccgttttttt atctggagga ttaatgacaa tcacggacct
    10681 ggtactgatt cttttcatcg ccgcacttct ggccttcgcg atctacgatc agttcatcat
    10741 gccccgccgt aacggcccca ccctgctggc aattcctttg ctccggcgtg gtcgcatcga
    10801 tagcgttatc ttcgtcggat tgattgtcat tcttatctat aacaacgtca cgaatcatgg
    10861 cgcgcttata acaacatggt tattaagtgc actggctctg atgggttttt atatattctg
    10921 gattcgcgtt ccgaagatca tctttaaaca aaaaggtttt ttcttcgcca atgtctggat
    10981 tgaatatagc cgaatcaaag cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt
    11041 agaacagcgt cggctgttaa tccgcgttcg aaatatcgac gatttggaaa aaatttataa
    11101 gcttctcgtt tcaactcaat aagttatgaa tttagccaaa gctatgttta gtgtattttt
    11161 aataatcata catagcttag gctatattag ctcttccctt atttgttatt tattttaacg
    11221 tttcattgat atataaatct aaatg
//
LOCUS       DACADA010000142        11224 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.144, whole genome shotgun sequence.
ACCESSION   DACADA010000142 DACADA010000000
VERSION     DACADA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.144"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            70..1374
                     /locus_tag="ISB17_003753"
     CDS             70..1374
                     /locus_tag="ISB17_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP1981640.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(1445..2857)
                     /gene="ftsP"
                     /locus_tag="ISB17_003754"
     CDS             complement(1445..2857)
                     /gene="ftsP"
                     /locus_tag="ISB17_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAP1981641.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(2932..3669)
                     /gene="plsC"
                     /locus_tag="ISB17_003755"
                     /gene_synonym="parF"
     CDS             complement(2932..3669)
                     /gene="plsC"
                     /locus_tag="ISB17_003755"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAP1981642.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(3904..6162)
                     /gene="parC"
                     /locus_tag="ISB17_003756"
     CDS             complement(3904..6162)
                     /gene="parC"
                     /locus_tag="ISB17_003756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP1981643.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYVAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6300..7907)
                     /locus_tag="ISB17_003757"
     CDS             complement(6300..7907)
                     /locus_tag="ISB17_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1981644.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(8016..8498)
                     /locus_tag="ISB17_003758"
     CDS             complement(8016..8498)
                     /locus_tag="ISB17_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1981645.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPDAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            complement(8551..8943)
                     /gene="ygiW"
                     /locus_tag="ISB17_003759"
     CDS             complement(8551..8943)
                     /gene="ygiW"
                     /locus_tag="ISB17_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAP1981646.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            9095..9754
                     /gene="qseB"
                     /locus_tag="ISB17_003760"
     CDS             9095..9754
                     /gene="qseB"
                     /locus_tag="ISB17_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAP1981647.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9751..11100
                     /gene="qseC"
                     /locus_tag="ISB17_003761"
     CDS             9751..11100
                     /gene="qseC"
                     /locus_tag="ISB17_003761"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAP1981648.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAKQGGFEAKVSW"
     gene            complement(11146..>11224)
                     /locus_tag="ISB17_003762"
     CDS             complement(11146..>11224)
                     /locus_tag="ISB17_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HAP1981649.1"
                     /translation="LYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT         2650 a         2974 c         2821 g         2779 t
ORIGIN      
        1 tggtctggta ttcagtaatg aatattattg acaactatcg taaacggaat tttcattaac
       61 gagggaaata tggactttga atatatctac cccgtcttaa ttttatttgg cagttttgcc
      121 gtcatgctgg caataggtgt gccaattact tttgcgattg gtctttcttc gctgttatct
      181 attattactg ccttaccacc cgatgccgcc atttctgtga tttcgcaaaa gatgactgtg
      241 gggctggatg gctttacgct attagccatt cccttcttcg tgttagccgg aaacattatg
      301 aataccggtg gtatagccag acgactggtt aacctggcgc aagcattagt tgggcgtctt
      361 cctggctcac tggctcattg taatatcctc gcgaatacac tgtttggtgc gatttcaggt
      421 tcagccgttg cgtcagccgc cgcggtaggt gggattatgt caccactgca agaaaaagag
      481 ggatatgatc cggcgttttc agcagcggtt aatattgcct ctgcccccat tggcctgatg
      541 atcccaccga gcaatgtgtt gattgtttat tcccttgcca gtggcgggac ttctgtcgct
      601 gcgttgttct tagctggata tctgccgggc atcctcaccg ctgttgcctt aatgtttgta
      661 gctgcacttt atgcccgacg tcaccattat ccggtggccg agcgcattaa ttaccgtcag
      721 tttttacagg tattccgcga atcaattcct agcctgatgc ttatttttat cattattggc
      781 ggtattatcg cgggggtatt tacgcctaca gaagcatcgg caattgcggt aatttatagt
      841 ttagccctgg cgatgattta ccgggaaatc actcttaaga agctcaatga tattctgtta
      901 gattcggtag taaccagttc aattgttctg ttactggtag gctgctcgat ggggatgtca
      961 tgggccatga cgaatgctga tgttcctgag ttgatcaacg aactgattac ccgcgtttcg
     1021 gataacaaat gggtcattct gtttattatc aatatcattc tgttgatcgt tggtaccttt
     1081 atggatatca cgccggcgat cctgatattt acacctattt tcctgccgat cgcccagcat
     1141 ctggggattg atccgattca cttcggcatc attatggttt tcaacttaac cattggcctt
     1201 tgcacaccac ctgtcggcac cattctgttt gtgggatgca gcattggtaa ggtcagtatc
     1261 gacagggcaa taaaaccatt actgccgatg tttctggcat tgtttgtagt aatggcaatt
     1321 atttgttatt tcccgcagct tagtctgatg ctgccaggat tattttcgac ctgatacaca
     1381 atgaagactg catactgagt aattttcaat gccccaccac tctgtggggc attgaattgg
     1441 tccattacgg taccggattg accaacagtt gcccaatcga gccacggtcc gccatttcca
     1501 gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa
     1561 gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg
     1621 gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg aacgcttgcg
     1681 gctcgtccgc gcgcaccgtc cagcgttccc acgttccttg ctgcgcagta acatcaatac
     1741 ggttgacgtc ccacaactgc ccattaatcc ccgggtcatc acccagactg atatcacgac
     1801 ttcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg
     1861 tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagcgtagaa accagaatgc
     1921 tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg ccacaggtaa
     1981 tcgacacttc atcaccgttg ctcatatcca ccagaatctc acgacgctcg cccggtgcca
     2041 gcgaaagttg cttcaccgat acaggagcgg gcaggaatcc ctgatcgcca gaaatcacat
     2101 gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcaaca
     2161 gacgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca
     2221 gtagcgtatc gccaacaaag ccgccgcttc caggttcgtt atattctggc gtaccaaagt
     2281 tatccagccg tttatcctga ataatgacag gaaaatcatc cacaccataa tggttgggga
     2341 taggcagcga tttactgact tcatcttcca ccagccacat tccggcaagg ccgttataga
     2401 cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc
     2461 gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca
     2521 tcagcgggcc aggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt
     2581 tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac
     2641 gaccattgat tccccaaacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt
     2701 gtacagtcat aaacagcggt tgcccacggc gagattcaag cagcggcgga acgggtagcg
     2761 gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa agtgcaatcc
     2821 ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct
     2881 aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt
     2941 tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat
     3001 cgaacgacaa tgggcagcca actcgcgaac ctgatctttg ccatactgac tgacgtcaat
     3061 tggcggcagc atttcgacaa tcaccagacc gttgtgtagc cgattaagat taatcttatt
     3121 cgaagttgta gagacgcaca cgggtataat cgggacgccc gccgcaattg ccgcgtgaaa
     3181 tgccccggtc ttgaacggca gcaggccgcg accacggctg cgggttcctt ccgggaacat
     3241 ccagatggaa atacggcgtt ttttgaagtg attcactact tccgcaatag tgccgtgagc
     3301 tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccaataca actgcccgaa
     3361 gaaggggatc cacagcaaac tctttttacc taccgtcact gtcggcggct gcacaatgtt
     3421 cgatgccgtc accatgtcat aattgttctg gtggttagca atatagatag cattgccgta
     3481 gttttcagcg tctgcaggtt tgcggcactc cactttcagg ccgaaaagcg gcgcaagacg
     3541 accaaacata tgcccaaagg tggccacatg tttcggatta cgcgggctga aaaggcagta
     3601 aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat
     3661 atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac ctgaaaatca
     3721 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
     3781 ataaacaacg gtttcaaggg aatttttatc gtttccgggg attgaatacg ctgccggatg
     3841 acgacttaac gtttcatccg gcgttccttg caagcgggag gaaacagcgc cctccccggc
     3901 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
     3961 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
     4021 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
     4081 cggcggcaga acgtacagtt gcgccaggcc atcctcgcca cgcgcggctt ctgccgatgg
     4141 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
     4201 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
     4261 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ctgcacggtt
     4321 acgcgccacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
     4381 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgctccag gcggcaacgt
     4441 taatttgccg gtgagcggct cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
     4501 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttgctct tacctttcac
     4561 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
     4621 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
     4681 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttca tgcaacggcg aacgacgttc
     4741 gtcaccgtag ccttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
     4801 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
     4861 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
     4921 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcatt
     4981 acgaatgatc tcaatcactt cgtcgatatt gagaaacgcc accagcaaac cttcgaggat
     5041 atgcaggcgc ttgagaactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
     5101 gcggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
     5161 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcaaagag
     5221 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
     5281 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
     5341 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gcaatgcgct
     5401 gataaccacc gcgccatctt ccttcttcca aaccgcgcgc atacgcactg aaccacgtcc
     5461 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
     5521 atccggcccc tgcacgatat ccagcagctg atcgagcgtg gttttcggct ggtcgattaa
     5581 ggcaattacc gcctgagcca cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
     5641 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gcagcatttt
     5701 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
     5761 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
     5821 cgatttcgga tcgtccggcg ccccccagtt cccctgacca tcaaccagcg gataacggta
     5881 agagaacggc tgcgccatca ggaccatcgc tacataacag gcaatatcgc cgtgcggatg
     5941 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
     6001 caggcccagc tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
     6061 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
     6121 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
     6181 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
     6241 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccggcggcg ataaaggcat
     6301 caatgtgcct tgatatacaa ctctttgctg tagacataat cctggggatt atgcagcgga
     6361 aaaccgccaa cgtagggttt aagcagtttg attaacggct gatagtagat gggaatcagc
     6421 ggtgcctgct ggttgatgat cacttctgcc tgctgataca gcgcattacg ctttgttgca
     6481 tcagccgttt gtgccgcctg gtttagtaag gcgtcatact gcgcgttttt ccagtggccg
     6541 acgttttctt cactatcgct tttgagcgtg ttcaggaagc tggaggcatc attgtacgtc
     6601 gcatcccacg actgccgaga cagcataaaa tcaccggctc gtcgggcatc aagataggtt
     6661 ttccactcca ttgtgcgcag cgtcacctgt gcacccagcc attttttcca ttcggaagac
     6721 aacgctatcg cggtcttttc atgcagatcg tacttgttgt agaacagctc aaagcgtagc
     6781 ggatgagagg cgtcgtatcc cgcctgtttc agcaagactt ttgccatcgc gacgcgctca
     6841 ctcattggct tttgcagttc atcgaacgtc gtcgcgctaa agccttttac ctctggcggc
     6901 gtcagcgtgg ttgcgggcgt tctcaacccc agtacctttt gcgcaataag ctgtcgatca
     6961 accgtaagat atagcgcccg acgcacccgc acatcgttaa atggcggttt ctcaaggtta
     7021 aagttgtaat attcgctgtt cagacgcggg ataattcgta gctcgccagg cagtgatttt
     7081 tcaatggcgg gaatttgctg cgccggaacc caggtgagat cgacctctcc cgcgcgatag
     7141 cggttatagc cggtgaccga attatccagc gccagatact caacctgttg caatactgta
     7201 tgttgcgcat cgcggtactt tggatttttg cgtgcagtaa tcttttcgtt aactacccat
     7261 ttatcaagca caaaggcacc gttgtaaacc atgttctctg gcttactcca gctatcgcca
     7321 tgtttagcga tgacatgatg aggaaccggg aacagcgtcg gccaggcgag catcgtcgtg
     7381 aaccaaggaa ccggctgctc aagcgtaact tcaagagtac gatcatccat cgctttgaca
     7441 cccagcgatg taacatctgc tttacccgca acaatagccg cggcattgtt aatgtgcgcc
     7501 tgtgccagat agccagcaaa agggcttgcc gttttcgggt caaccgcgcg ctgccagccg
     7561 aggacaaaat cctctgccgt cagaggctga ccgtctgacc actgcaaacc gctacgcaga
     7621 tggaaaatat agcgcttgcc gccgtcaagt atctcccagc gttcagcctg agcgggctgc
     7681 acctggcctt caccgtccat ccataccaga ccttcaaaca gatccagcac aatctgcgcg
     7741 gcggtattct cctctacctt ttgcgggtcg agcgtgcctg ggtcgctatg attgttgtaa
     7801 cgaaagactt gttgcggggc gagcggtgtg ttggcgggaa cgtcagcagc gtagagagga
     7861 gccgcactta ccaggctgac cagccataat aaatttcgcg tatacatggt attccttacc
     7921 ggagaaatta taagcccgac aacaatacgc ctgtggcatt gtttgctcaa acttatcgcg
     7981 agtgatttgg ctcacactcc ggtaaagatt ccccatcacg ctaacggtac ataaacatcc
     8041 atttccagtc gctgctctgt catcccttcg gcaagattgg tgtagtgaaa cagaatcggt
     8101 gctttacgca ttttctcgcc gcttgcaggc agccagtggc gaatgatgcc ccatatcgtg
     8161 tgtgaaatat cgtccagctc gccaacatgg cgggccacag cataacgtcc accggtaagt
     8221 tcaccattac ttacgccata acgattatcg gggatcggtt cgctaacgct gccgcagata
     8281 tcaaagcgaa acgcatctgg tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc
     8341 tggctttggt taacaggaga aagccccgtc tctttacgcc acatgataaa tttcgccacg
     8401 ctgtagttga gcaactcagg gctacagcgg tgcggcaaca tcgccacagg gattgatggg
     8461 aaatcgataa tatttacatc cagtgtcagg tttgtcattt tgcctccctg cgaaattatt
     8521 cccgggaacg cgtcccggga gcggtaacaa ttacggattt actttgcgga tctgtttgac
     8581 gtcaatttca acagagttcc agtctttatc gacttcaccc tgaatctcaa ccgtatcttt
     8641 cggcgtcacc gtcacgccgt tccagcgttt atggtcgata tcaacattga tagtaccgct
     8701 ggcatctttg aacacgtaga gatcgtcaga gatgcgttca acgatattgc cgcgtagggt
     8761 tacccaggtg tcgtcacgca gggattttgc gctttctaca gtcgttacgc tgccgttcgg
     8821 cccctggaat cctccggcct gactttgcgt tgccgatggg ccagaaaaac ctccctgctc
     8881 tgctgccata accggtgcac tgcacagggc cattactgca attactgctg cgaatttttt
     8941 catgtttatt actcccttta atgtctgttt ccgagcattt aacaagatag tccttaacaa
     9001 cttcttaagg gaaaaaaata aaatttaatg ctgtacagag cgcgttacaa cacggtttac
     9061 tggcagcaaa tacggttatc gcagggatga aaaaatgcga attttactga tagaagatga
     9121 catgctgatt ggcgacggca tcaaaacggg ccttagtaaa atgggtttta gcgtcgactg
     9181 gtttacacaa ggtcgtcagg gaaaagaggc gctatatagc gcaccttatg atgcggtgat
     9241 cctggattta accttaccag gcatggatgg tcgcgatatt ttgcgcgaat ggcgagaaaa
     9301 aggtcagcgt gagcctgtat tgatcctgac tgcgcgcgat gcgctggcgg aacgtgtaga
     9361 agggctgcgt ctgggagctg acgattatct gtgtaaacct tttgcgttga tagaagtcgc
     9421 cgccaggctg gaagctctga tgcgccgaac caacggccag gccagcaacg agctgcgcca
     9481 cggcaacgtc atgctcgacc ccggcaaacg tatcgccacg ctggctggcg aacccttaac
     9541 gctgaaacca aaagaatttg ccctgctgga attactgatg cgtaacgctg gtcgggtact
     9601 gccgcgcaaa ctgattgaag agaaactgta tacctgggac gaagaggtca ccagtaatgc
     9661 cgttgaagtg catgtgcatc atctgcgacg caaactcggt agtgatttta ttcgtaccgt
     9721 gcatggtatt ggctacacat taggtgagaa atgaaattta cccaacgtct tagtctgcga
     9781 gtcaggctga cgctaatctt tttaattctg gcctcggtga cctggctgct ttccagcttt
     9841 gtcgcctgga aacaaacaac ggataacgtc gatgagttgt tcgacaccca actgatgctg
     9901 tttgccaaac ggttaagtac gctcgatctc aacgaaatca acgcggcgga tcgcatggca
     9961 cagacgccaa ataaattaaa acacggtcat gttgatgacg atgcgctgac ttttgccatc
    10021 tttacccacg acggcagaat ggtccttaac gatggcgata acggagaaga tattccctat
    10081 agctatcagc gggaaggttt tgctgacggg caactggtcg gtgaagacga tccctggcgt
    10141 tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg ttgtgggtca ggaatgggaa
    10201 taccgtgaag acatggcgct ggcgattgtt gccggacaat tgatcccgtg gctggtcgca
    10261 ctgccgatta tgttaatcat catgatggtg ctactgggtc gtgaactcgc gccgctgaac
    10321 aaactggcgc tggcactacg tatgcgtgac ccagactcgg aaaaaccact aaacgcgacg
    10381 ggcgtaccca gcgaagtgcg cccactggtt gagtcgctaa atcaactgtt cgcccgcaca
    10441 catgcgatga tggttcgtga acgacgcttt acctccgacg cagctcacga acttcgtagc
    10501 ccgttaacgg cgctgaaagt gcaaaccgaa gttgctcagc tctctgacga tgatccgcag
    10561 gcgcggaaaa aagcgctgct ccaattacat tccgggatcg atcgcgctac tcgtctggtt
    10621 gatcaactgc tcacgctatc gcggctggac tcactggata accttcagga cgtcgcggag
    10681 atcccgcttg aagatctcct gcaatcgtcg gtgatggata tttaccacac ggcgcagcag
    10741 gcgaacattg acgtgcgact gacactcaat gccaacggca tcaaacgcac cggacaaccg
    10801 ctattgctaa gtttgttggt gcgaaatttg ctggataacg ccgtgcgtta cagtccacaa
    10861 ggtagcgtgg tagacgtcac gctgaatgcc gataatttca tcgtgaggga taacggccct
    10921 ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa
    10981 accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa attgcatggc
    11041 atgaatgttg attttgggaa tgcgaaacaa ggtggatttg aagcgaaggt aagctggtaa
    11101 cttctctgtc tatgagagcc gttaaaacga ctctcataga ctttactaat agcaaaatat
    11161 aaaccgtccc caaaaaagcc accaaccaca acccagacaa ataacgagcg agaatacata
    11221 tagc
//
LOCUS       DACADA010000143        11088 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.145, whole genome shotgun sequence.
ACCESSION   DACADA010000143 DACADA010000000
VERSION     DACADA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.145"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..329
                     /locus_tag="ISB17_003763"
     CDS             <1..329
                     /locus_tag="ISB17_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HAP1981650.1"
                     /translation="FDFEVMALKPLISPTWLNILYIMFGVHGGSYFLGIVETITFLAL
                     SIGFFRPAAGILGDIIVIVTGLVTLSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKN
                     YLNIKN"
     gene            complement(472..685)
                     /locus_tag="ISB17_003764"
                     /pseudo
     CDS             complement(472..685)
                     /locus_tag="ISB17_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1047..2240)
                     /locus_tag="ISB17_003765"
     CDS             complement(1047..2240)
                     /locus_tag="ISB17_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981651.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2376..4100
                     /gene="iucA"
                     /locus_tag="ISB17_003766"
     CDS             2376..4100
                     /gene="iucA"
                     /locus_tag="ISB17_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAP1981652.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4101..5048
                     /gene="iucB"
                     /locus_tag="ISB17_003767"
     CDS             4101..5048
                     /gene="iucB"
                     /locus_tag="ISB17_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAP1981653.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5048..6790
                     /gene="iucC"
                     /locus_tag="ISB17_003768"
     CDS             5048..6790
                     /gene="iucC"
                     /locus_tag="ISB17_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAP1981654.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6787..8064
                     /gene="iucD"
                     /locus_tag="ISB17_003769"
     CDS             6787..8064
                     /gene="iucD"
                     /locus_tag="ISB17_003769"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAP1981655.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            8146..10347
                     /gene="iutA"
                     /locus_tag="ISB17_003770"
     CDS             8146..10347
                     /gene="iutA"
                     /locus_tag="ISB17_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAP1981656.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2652 a         2897 c         2890 g         2649 t
ORIGIN      
        1 ggtttgattt tgaagtgatg gccctcaagc ccctgatttc tcccacgtgg ctgaatatcc
       61 tgtacattat gtttggtgtt catgggggga gctatttcct cggcatagta gaaactatca
      121 cttttctagc actgagtatc ggcttcttca ggcctgctgc tggcatactg ggagatatta
      181 tcgttattgt tacaggttta gtcacactaa gtctgttacc acaactggga aaagttgaca
      241 gttttgtcat caaagatgtg ctgctgttag gtgctggtag tgtcctgctg aaatatgatt
      301 tgaaaaatta tttaaatatc aaaaactgat ttttcacttc ggtgatgaac gtttgcgtat
      361 tttcatggaa agagattacc taatccccct cgttctcctt catgttaata cgcaacgtta
      421 tggaaaacaa aaataccctg agtaaaaagg tccgtaggca ggcgagtact tttattttcc
      481 gctagtttta ctttcccatc gaaggcccca tacatcacta cgcccacgaa acaaccgacg
      541 aaataacgca actttctcat ccgtggataa tacggaaacg cactgcacag gactctgcgg
      601 tttacgtcgc cattcaatcc cataagcttc aagtgatgtc ttccctcagt gctgctaatt
      661 catcactgtc agatttatga accatattcg ttacctgtat ccgaaccact gaagcaccac
      721 agcagaaatt cgggtcccct acactgatta cagcgccccc tctgctgcct attaactcaa
      781 gcacctccca acaacttacg gaaacttaat tctaatacag ctgtaattca gttgtgtcgt
      841 ggtcggccgc ggttactttt ttcgggaagt ctgctacgca tcagaataat accgtgttct
      901 ttctgatcct gtttagccag gtagctaagg gcgtaacgta aaccaggcgg gggcagcaag
      961 ccagaacact gcctgacatg ttttcatcag acgcaggtcc tctctcatat cctctgattt
     1021 actccagtaa ggtgatgatc atatctttat ttgatagtac ggtaatgcca gataaataca
     1081 gacacaatgg taaataatat agttgcaaat aatgctgcca taatcagcgc gattgcgaca
     1141 cctgttttcg ataccagcgg caccagcaaa gcgcacagtc catagccaag tgtgtgactg
     1201 gtagcgagcc agcccgcgcc tgcgccatca gccagcttat cattcagtag aagttgataa
     1261 gcgggtgttg ccagagctgc tccaaatgac agcactgcac agcccgggta aaacaaccat
     1321 agctgttcgg agagcatgat agccagacca ccactcatca acactccggc actcaggagc
     1381 agggccaccg gagtcaggcg ctgcggacgg agtaccccga actgcgcgat aagcgcagct
     1441 actgcggaca gacccaacag ccaggccacc tgttggctaa tggcggtggt atccgtggcg
     1501 aactggcgag taagggcagg cgaaagtcca agctgcatca tgctcaccgc cgcagccagt
     1561 aatattgcgc aaagcagata aggcagacaa tcccgcttca gactgacgct cttgcactcc
     1621 ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca tcagcagcgc cagcactggg
     1681 gctgccatca gcagccccag tggcgccaga ggatgaatgg ccagcattgc tgccgcgcac
     1741 agcggcccga atagcctgcc gcaactcagg ccggagctga tggttgccag agcggccatg
     1801 cgattccctt ctccagctct ctgcaatgcc cagacctgac aggctggcac cattgccgac
     1861 acggtcagcc cgtaggcgat ccgggcgatg attaatattc ccagtcccac cgctgttgtt
     1921 atggcgctgg tagccatcag cacgcttccc agccctagca gggtaaagct tatgccatat
     1981 cctcccagag cccacaacac cacaggttta ctgccagtac gggaaatttg ctttccccag
     2041 cacggggacg atggcagaaa cagcattgag ccaagcatca gcagggcagc ccatacagac
     2101 agactcagat ttgtctgtat gaccagaaca gggagcacaa ccagcagacc gttctgtccg
     2161 ataccgagaa gcccggcact gaacgcaagt ggccagcagg acagtggttt ttggggcgta
     2221 tcttcgatcc ctggtgacat tataatgttt caactccatg tattaattgt gtttatttgt
     2281 aaaattaatt tatctgacaa taacatttat tattgataat gagaatcatt attgacataa
     2341 ttgttattat tttgctgtgt gggagctgtt tgactatgac cctgccctct gaaaaaccag
     2401 ccacagatgt ggctgcgcag tgcttcctga atgcactgat tcgtgaaacc acagactgga
     2461 aactgacaga atacccgcca gacgaattgc tcatcccgct ggatgagcag aaatcgctcc
     2521 atttcagagt ggcttatttc tccccaaccc agcatcaccg ctttgcattt cctgcacgtc
     2581 tggtcacggc atcaggcagt tatcctgtcg actttaccac tctctcccgg cttattattg
     2641 ataagctacg ccatcaactg tttctgcccg ttcccctctg cgaaactttc caccagcgcg
     2701 tgctggaaag ccatgtccat acgcaacagg caattgatgc ccgtcatgac tgggccgctc
     2761 tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc gctgctgaca ggacacgctt
     2821 tccaccctgc gcctaagtct catgaaccgt ttaaccggcg ggaggctgaa cgctacctgc
     2881 ctgatatggc accccacttc ccactgcggt ggttttcggt ggataaaacg caaatcgccg
     2941 gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg atttgccgca gagaatgcgc
     3001 ctcagttact caacgaatta agtgacaacc aatggctgtt cccgttgcac ccgtggcagg
     3061 gggaatatct tttgcagcag gggtggtgcc aggcacttgt tgctaaaggg ctgattaaag
     3121 acttaggtga ggccggcacg tcgtggctgc cgaccacctc ttcccgttcc ctctactgtg
     3181 ccaccagccg cgatatgatc aagttctccc tgagcgtacg gctgaccaac tccatccgta
     3241 ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc acgcctggct caaaccgacg
     3301 gctggcagat gctacaggtc cgcttcccga ctttccgggt aatgcaggag gatggctggg
     3361 ccgggctgcg cgatcttaac ggcaacatca tgcaggaaag tctgtttgcc ctgcgtgaaa
     3421 atctgctggt ggatcagccg aaaagccaga ctaacgtact ggtctccctg actcaggccg
     3481 cacctgatgg cggtgattcg ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg
     3541 gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc atactgtcag caagtgctaa
     3601 agccgctgtt tacggctgaa gcggattacg gcctggtgct gctggcgcat cagcaaaata
     3661 ttcttgtcca gatgcttggg gatctgccgg tcggatttat ttaccgtgac tgtcagggca
     3721 gcgcttttat gcctcacgcg acagactggc tcgattctat tggcgaggcg caggcggaaa
     3781 atattttcac ccatgagcag ttgctgcgct atttccctta ttacctgctg gttaactcca
     3841 cttttgctgt gaccgctgcg ctgggggctg ccgggctgga cagcgaatcg aatctgatgg
     3901 ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt gactcataaa acctgtctca
     3961 actacgtgct ggaaagtccg tactggaacg taaaaggtaa ctttttctgt tatctgaacg
     4021 atcataacga gaacaccatt gttgaccctt cggtgatcta tttcgatttc gctaacccgc
     4081 tgcaggctca ggaggtctga atgtctgagg caaacattat tcacagcaga tatggactgc
     4141 gctgtgaaaa actcgacaag cccctgaatc ttggctgggg actggacaat agcgcggtgt
     4201 tgcactgtcc cggggagctg ccgacagggt ggctgtgcga tgcgctggat cagatattta
     4261 tcgccgctcc acaactgtca gcagtggctc tcccctgggc cgaatggcgt gaggagccac
     4321 aggcgctgac gctttttggg caggtaaaaa gcgacattat ccatcgtact gccttctggc
     4381 agttaccgtt atggttgagt tcaccggcaa accgggcctc cggtgaaatg gtttttgatg
     4441 cagagcgtga gatttatttc ccgcagcgcc caccccgtcc acagggtgag gtttaccgcc
     4501 gttacgatcc acgtattcgc aggatgctga gtttccgcat tgccgatccc gtttctgatg
     4561 cagaacgttt cacccgctgg atgaatgatc cgcgcgttga gtatttctgg gagcaaagtg
     4621 gctcactgga ggtacagacc gcctatctgg agcgccagct caccggtaaa catgcattcc
     4681 cgctgattgg gtgcttcgac gatcggccat tcagctattt cgaaatctac tgggcggcgg
     4741 aagaccgcat tggtcgtcac tattcgtggc agccctttga ccgtggcctg catctgctgg
     4801 ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag ctggctgcgc gggctgacac
     4861 attacctgct gctggatgag ccccgtacgc agcgcacagt actggagcca cgcaccgata
     4921 acctgcgcct gttccgccat cttgagcctg cgggataccg gacaattaaa gagttcgact
     4981 tcccacataa gcgctcgcgc atggtgatgg cggatcgtca tcacttcttc acggaggtcg
     5041 gtctgtaatg aatcacaagg actgggattt tgttaaccgt cggctggtgg caaaaatgtt
     5101 gtctgagatg gagtatgagc aggttttcca cgcggaatct cagggcgatg accactactg
     5161 cattaacctg ccgggagcac aatggcgctt catcgctgaa cgtggtatct ggggctggct
     5221 gtggattgat gcccaaacgt tgcgctgcac ggacgaacca gtactggcgc agacgctgct
     5281 gatgcagcta aagccggtgc tgtcaatgag tgatgccact gttgcagagc atatgcagga
     5341 tttgtatgcc acgctactgg gcgacctgca actgctgaag gcccgtcgcg gactgagtgc
     5401 cagtgacctg attaatcttg atgccgatcg tctgcaatgc ctgctgagcg gtcatcctaa
     5461 attcgttttt aataaaggtc gccgtggctg gggcaaagag gcgctggaac gatatgcgcc
     5521 agagtatacc aacaccttca gactgcactg gctggcggta aaacgtgaac atatgatctg
     5581 gcgctgcgat aacgatctgg atattcagca gttgttgact gccgcaatgg atccgcagga
     5641 gtttacacgc ttcagtcagg tctggcagga aaacggactg gatcacaact ggctgccgct
     5701 gccggtacat ccgtggcagt ggcagcaaaa aatcgctacc gacttcatcg ctgattttgc
     5761 cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag tggctcgccc agcagtcgct
     5821 gcgtaccctg accaacgcca gtcgccgggg cgggctggat atcaagctgc cgctgaccat
     5881 ctacaacacc tcatgctacc gggggatccc tggcagatac atcgctgccg gaccactggc
     5941 ttcacgctgg ctacaacagg tttttgcgac cgacgccacc ctggtgcaaa gcggcgcagt
     6001 gatacttggt gaaccggctg caggctatgt ctcccatgaa ggctatgccg cgcttgcccg
     6061 ggctccctat cgctaccagg aaatgcttgg tgttatctgg cgggagaatc cgtgccgctg
     6121 gctgaaaccg gatgaaagtc cggttctgat ggcaacactg atggagtgcg acgaaaacaa
     6181 tcagccgctg gcaggcgcat atatagaccg ctccgggctg gacgctgaaa cctggcttac
     6241 gcaactgttc cgggtggtcg tggttcctct gtatcacctg ctttgccgct acggtgtcgc
     6301 gcttattgca catggacaaa atataactct cgccatgaaa gagggggttc cacagcgtgt
     6361 tctgctgaaa gacttccagg gcgatatgcg actggtgaaa gaagagttcc ccgaaatgga
     6421 ctctttgcct caggaggttc gtgatgttac atcccgcctg agtgcggact acttaatcca
     6481 tgatttgcag acgggtcact tcgtgacagt actgcgtttt atttcgccac tgatggttcg
     6541 tcttggcgta cctgaaaggc gattttatca actgctggca gcagtgttga gtgattacat
     6601 gaaaaaacat ccacaaatgt cagagcgttt tgcgcttttc tcacttttca ggccacaaat
     6661 cattcgcgtg gtgctgaacc cggtaaaact gacctggcct gatctggatg gcggcagccg
     6721 catgctgccg aattaccttg aggatctgca aaatccgctg tggctggtaa ctcaggaata
     6781 tgaatcatga aaaaaagtgt cgattttatt ggtgtaggga cagggccatt taatctcagc
     6841 attgctgcgt tgtcacatca gatcgaagaa ctggactgtc tcttctttga tgaacatcct
     6901 catttttcct ggcatccggg tatgctggta ccggattgtc atatgcagac cgtctttctg
     6961 aaagatctgg tcagtgctgt tgcacctaca aatccctaca gttttgttaa ctatctggtg
     7021 aagcacaaaa agttctatcg cttccttaca agcagactac gtacagtatc ccgtgaagag
     7081 ttttctgact acctccgctg ggctgctgaa gatatgaata acctgtattt cagtcatacc
     7141 gttgaaaaca ttgatttcga taaaaaacgt cgattgtttc tggtgcaaac cagccaggga
     7201 gaatattttg cccgcaatat ctgccttggt acaggaaaac aaccttattt accaccctgt
     7261 gtgaagcata tgacacaatc ctgtttccat gccagtgaaa tgaatcttcg tcggccggac
     7321 cttagtggaa aacggataac cgtggttggt ggaggacaga gtggtgcaga cctgttcctt
     7381 aatgcattac gcggggaatg gggagaagcg gcggaaataa actgggtctc ccggcgtaat
     7441 aattttaacg cactggatga ggctgctttt gctgatgagt attttacacc tgaatatatt
     7501 tcaggcttct ccggactgga ggaagatatt cgccatcagt tactggatga gcagaaaatg
     7561 acatcggatg gcatcactgc cgattcttta ctgaccattt atcgtgagtt gtaccaccgt
     7621 tttgaagttc tgagaaaacc aagaaatatc cgtctgctac ccagccgctc ggtaacaact
     7681 ctggaaagta gtggtcctgg ctggaagttg ctgatggagc atcatctgga tcagggcagg
     7741 gagagcctgg aaagtgatgt ggtgattttc gccacaggtt accgttctgc gttgccacaa
     7801 atacttccct cactgatgcc cctgatcacc atgcacgata agaacacctt taaagtgcgt
     7861 gatgacttca ctctggaatg gagtggcccg aaagagaaca acatcttcgt ggtcaacgcc
     7921 agtatgcaaa cccatggcat cgccgaaccc cagctcagcc tgatggcatg gagatctgca
     7981 cgtattctta atcgcgtaat gggacgtgat ttattcgatc tcagtatgcc gcccgccctg
     8041 attcagtggc gcagcggcac ctagggaaaa cgcagccgga ggctgcttct ttaactcgct
     8101 acacagcatc tttgggctga ttttttccgc ccgtatggag gaataatgat gataagcaaa
     8161 aagtatacgc tttgggctct caacccactg cttcttacca tgatggcgcc agcagtcgct
     8221 caacaaaccg atgatgaaac gttcgtggtg tctgccaacc gcagcaatcg caccgtagcg
     8281 gagatggcgc aaaccacctg ggttatcgaa aacgccgaac tggaacagca gattcagggc
     8341 ggcaaagagc ttaaagacgc actggctcag ctgatccctg gccttgacgt cagcagccgg
     8401 agccgcacca actacggtat gaatgtgcgt ggccgcccgc tggtcgtgct ggttgacggc
     8461 gtgcgtctca actcttcacg taccgacagc cgacaactgg actctataga tccttttaat
     8521 atcgaccata ttgaagtgat ctccggtgcg acgtccctgt acggcggcgg cagtaccggt
     8581 ggcctgatca acatcgtgac caaaaaaggc cagccggaaa ccatgatgga gtttgaggct
     8641 ggcaccaaaa gtggctttag cagcagtaaa gatcacgatg aacgcattgc cggagctgtc
     8701 tccggcggaa atgagcatat ctccggacgt ctttccgtgg catatcagaa atttggcggc
     8761 tggtttgacg gtaacggcga tgccaccttg cttgataaca cccagaccgg cctgcagtac
     8821 tccgatcggc tggacatcat gggaaccggt acgctgaaca tcgatgaatc ccggcagctt
     8881 cagttgatca cacagtacta taaaagccag ggcgacgacg attacgggct taatctcggg
     8941 aaaggcttct ctgccatcag agggaccagc acgccattcg tcagtaacgg gctgaattcc
     9001 gaccgtattc ccggcactga gcggcatttg atcagcctgc agtactctga cagcgctttt
     9061 ctgggacagg agctggtcgg tcaggtttac taccgcgatg agtcgttgcg attctacccg
     9121 ttcccgacgg taaatgcgaa caaacaggtg acggctttct cttcgtcaca gcaggacacc
     9181 gaccagtacg gcatgaaact gactctgaac agcaaaccga tggacggctg gcaaatcacc
     9241 tgggggctgg atgctgatca tgagcgcttt acctccaacc agatgttctt cgacctggct
     9301 caggcaagcg cttccggagg gctgaacaac aagaagattt acaccaccgg gcgctatccg
     9361 tcgtatgaca tcaccaacct ggcggccttc ctgcaatcag actatgacat caataatctc
     9421 tttaccctca acggtggcgt acgctatcag tacactgaaa acaagattga tgatttcatc
     9481 ggctacgcgc agcaacggca gattgccgcc gggaaggcta catccgccga cgccattcct
     9541 ggcggctcag tcgattacga caacttcctg ttcaacgccg gtctgctgat gcacatcacc
     9601 gaacgccagc aggcatggct caacttctcc cagggcgtgg agctgccgga cccgggtaaa
     9661 tactatggtc gcggcatcta tggtgctgca gtgaacggcc atcttcctct aacaaagagt
     9721 gtgaacgtca gcgacagcaa gctggaaggc gtgaaagtcg attcttatga gctgggctgg
     9781 cgctttactg gcaataatct gcgcacccaa atcgcggcct actattcgat ttctgataag
     9841 agcgtggtgg cgaataaaga tctgaccatc agcgtggtgg acgacaaacg ccgtatttac
     9901 ggcgtggaag gtgcggtgga ctacctgatt cctgatactg actggagtac cggagtgaac
     9961 ttcaacgtgc tgaaaactga gtcgaaagtg aacggtacct ggcagaaata cgatgtgaag
    10021 acagcaagcc catcaaaagc gacagcctac attggctggg caccggaccc gtggagtctg
    10081 cgcgtgcaga gcaccacctc ctttgacgtg agcgacgcgc agggctacaa ggtcgatggc
    10141 tataccaccg tggatctgct cggcagttat cagcttccgg tgggtacact cagcttcagc
    10201 attgaaaacc tcttcgaccg tgactacacc actgtctggg ggcagcgtgc accactgtac
    10261 tacagcccgg gttacggccc agcgtcactg tacgactaca aaggcagggg ccgaaccttt
    10321 ggtctgaact actctgtgct gttctgacgg ggatgctttt gcaacaaggg tatgctgtaa
    10381 acaacatggt ccttggtggc catgttgttt acagccagta taatatacag ccttaacaag
    10441 atattccgtc tccggaatgg actgttcacc actgaataac attatgtctg ttttatggat
    10501 ctttgactga aatttatcgc atatcctcat ttcattgtcc gtctgaaaac taaccttaca
    10561 agtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctgggagg atgatctgcg
    10621 cccaatatcc tgagccggaa ctttgctgca atgaaggcca acgaaaaatg ggggagtatg
    10681 ttatagagta cttgctacag ggtaaaaaga ggtacctgtc gctggttctc cgttcagttt
    10741 aaccgggaga ttttctcctg cgcctgtcat aaagtccggt aatggagatt gttaatacga
    10801 tggtgcacga tgcattgtca gtgcccggcc cggaggatgc cccgtagcta catacggatc
    10861 agggttggca atatcgaatg gcaggctata ggcaaagtta aagccccatg gagtagcaca
    10921 aaatatgccg cacaaaggaa acggtctgaa taacgcagcg atggagaact tcttcggcac
    10981 actgcaaaat cgcatcgtgg tcgatcgcta attcgtcgtg aatcactgaa cgatgttcca
    11041 tgcgaacaga tattcgatca tatcgaaata ttaaacatta cccctccc
//
LOCUS       DACADA010000144        10939 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.146, whole genome shotgun sequence.
ACCESSION   DACADA010000144 DACADA010000000
VERSION     DACADA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.146"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            281..685
                     /gene="sdhC"
                     /locus_tag="ISB17_003771"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             281..685
                     /gene="sdhC"
                     /locus_tag="ISB17_003771"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1981657.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            679..1026
                     /gene="sdhD"
                     /locus_tag="ISB17_003772"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             679..1026
                     /gene="sdhD"
                     /locus_tag="ISB17_003772"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAP1981658.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1026..2792
                     /gene="sdhA"
                     /locus_tag="ISB17_003773"
     CDS             1026..2792
                     /gene="sdhA"
                     /locus_tag="ISB17_003773"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAP1981659.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2808..3524
                     /locus_tag="ISB17_003774"
     CDS             2808..3524
                     /locus_tag="ISB17_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAP1981660.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3886..6687
                     /gene="sucA"
                     /locus_tag="ISB17_003775"
     CDS             3886..6687
                     /gene="sucA"
                     /locus_tag="ISB17_003775"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP1981661.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6702..7919
                     /gene="odhB"
                     /locus_tag="ISB17_003776"
     CDS             6702..7919
                     /gene="odhB"
                     /locus_tag="ISB17_003776"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP1981662.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8013..9179
                     /gene="sucC"
                     /locus_tag="ISB17_003777"
     CDS             8013..9179
                     /gene="sucC"
                     /locus_tag="ISB17_003777"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP1981663.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9179..10048
                     /gene="sucD"
                     /locus_tag="ISB17_003778"
     CDS             9179..10048
                     /gene="sucD"
                     /locus_tag="ISB17_003778"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP1981664.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10152..10874)
                     /gene="mngR"
                     /locus_tag="ISB17_003779"
     CDS             complement(10152..10874)
                     /gene="mngR"
                     /locus_tag="ISB17_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAP1981665.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
BASE COUNT         2595 a         2822 c         3016 g         2506 t
ORIGIN      
        1 tgttcgggat aaaacccgac aaactatatg taggttaatt gtaatgattt tgtgaacagc
       61 ctatactgcc gccaggtctc cggaacaccc tgcaatcccg agccacccag cgttgtaacg
      121 tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg cagttataga aaggacgctg
      181 tctgacccgc aagcagaccg gaggaaggaa atcccgacgt ctcctggtaa cagaaagtta
      241 acctctgtgc ccgtagtccc cagggaataa taagaacagc atgtgggcgt tattcatgat
      301 aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta cagaccatcc ggttccccgt
      361 cacggcgata gcgtccattc tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg
      421 catcctgctg tggcttctgg gtaccagcct ctcttcccct gaaggtttcg agcaagcttc
      481 cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc
      541 atatcacgtc gtcgtaggta ttcgccacat gatgatggat tttggctatc tggaagaaac
      601 attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg tgctttcact
      661 tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa tggcgtacat
      721 gatttcatcc tcgttcgtgc taccgctatc gtcctgacgc tctacatcat ttatatggtc
      781 ggttttttcg ctaccagtgg cgagctgaca tatgaagtct ggattggttt cttcgcctct
      841 gcgttcacca aagtgttcac cctgctggcg ctgttttcta tcttgatcca tgcctggatc
      901 ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg ccttgcgcct gatgctgcaa
      961 ctggtgattg tcgttgcact ggtggtttac gtgatttatg gattcgttgt ggtgtggggt
     1021 gtgtgatgaa attgccagtc agagaatttg atgcagttgt gattggtgct ggcggcgcag
     1081 gtatgcgcgc ggcgctgcaa atttcccaga gtggccagac ctgtgcgctg ctctctaagg
     1141 tcttcccgac ccgttcccat accgtttctg cgcaaggtgg tattaccgtt gcgctgggta
     1201 atacccatga agataactgg gaatggcata tgtacgacac cgtaaaaggg tcggactata
     1261 tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg attctggaac
     1321 tggaacatat gggcctgccg ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt
     1381 ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc acgtactgcg gcggcagctg
     1441 accgtaccgg tcacgcactg ttgcacacgc tttatcagca gaacctgaaa aaccacacca
     1501 ccattttctc cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc gcagtggtcg
     1561 gttgtaccgc actgtgcatc gaaactggtg aagtggttta ctttaaagct cgcgcgacag
     1621 tgctggcgac tggcggggca gggcgtattt atcagtccac caccaacgcc cacattaaca
     1681 ctggcgacgg tgtcggcatg gctatccgtg caggcgtacc ggtgcaggat atggaaatgt
     1741 ggcagttcca cccgaccggt attgccggtg cgggcgtact ggtcaccgaa gggtgccgtg
     1801 gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt tatggagcgt tatgcgccga
     1861 acgccaaaga cctggcgggc cgtgacgtgg tggcgcgttc catcatgatc gaaatccgtg
     1921 aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcaaa actgaaactt gaccatctgg
     1981 ggaaagaagt tctcgaatcc cgtctgccgg gtattctgga actctcccgt accttcgctc
     2041 acgtcgatcc ggtgaaagag ccgattccgg ttatcccaac ctgtcactac atgatgggcg
     2101 gtattccgac caaagtgacc ggtcaggcgc tgacggtgaa tgagaaaggc gaagatgtgg
     2161 ttgttccggg gctgtttgcc gttggtgaaa tcgcttgtgt atcggtacac ggcgctaacc
     2221 gtctgggcgg caactcgctg ctggacctgg tggtatttgg tcgtgcggca ggtctgcatc
     2281 tgcaagagtc tatcgccgag cagggcgcac tgcgcgatgc cagcgagtct gatgtagaag
     2341 cgtctctgga tcgcctgaac cgctggaaca ataaccgtaa cggtgaagat ccggtggcga
     2401 tccgtaaagc actgcaagaa tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg
     2461 cgatggcgaa agggcttgag cagttgaaag ttatccgcga gcgtctgaaa aatgcccgtc
     2521 tggatgacac ttccagcgag ttcaacaccc agcgcgttga gtgcctggaa ctggataacc
     2581 tgatggaaac ggcgtatgca acggctgttt ctgccaactt ccgtaccgaa agccgtggcg
     2641 cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt
     2701 atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc
     2761 cggcattccc gccgaagatt cgtacttact aatgcggaga caggaaaatg agactcgagt
     2821 tttcaattta tcgctataac ccggatgttg atgatgctcc gcgtatgcag gattacaccc
     2881 tggaagcgga agaaggtcgc gacatgatgc tgctggatgc gcttattcag ctgaaagaga
     2941 aagatcccag cctgtcgttc cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc
     3001 tgaacatgaa cggtaagaat ggtctggcct gtattacccc gatttcggca ctcaaccagc
     3061 cgggcaagaa gattgtgatt cgtccgctgc caggtttacc ggtgatccgc gatttggtgg
     3121 tagacatggg acaattctat gcgcaatatg agaaaattaa gccttacctg ttgaataatg
     3181 gacaaaatcc gccagctcgc gagcatttac agatgccaga gcagcgcgaa aaactcgacg
     3241 ggttgtatga atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga
     3301 atcccgataa gtttatcggt ccggcaggct tgttagcggc atatcgtttc ctgatcgata
     3361 gccgtgatac cgagactgac agccgcctcg acggtttgag cgatgcattc agtgtattcc
     3421 gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg
     3481 ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc gtaaaccgta ggcctgataa
     3541 gacgcacaag cgtcgcatca ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc
     3601 ctacaaaaga aatgcagaaa ccgtaggcct gataagacgc gcaagcgtcg catcaggcaa
     3661 acagcgccgg atgcggcgtg aatgccttat ccggcctaca aaagaaatgc aggaaatctt
     3721 taaaaactgc ccctgacact aagacagttt ttaaaggttc cttagcggac gcagaaaaca
     3781 cgacaagtcc gcaacaagtg aaccccggca cgcacatcag atatgaaatg tgcgtggtag
     3841 tatccacggc gaagtaagca taaaaaagat gcttaaggga tcacgatgca gaacagcgct
     3901 ttgaaagcct ggttggactc ttcttacctc tctggcgcaa accagagctg gatagaacag
     3961 ctctatgaag acttcttaac cgatcctgac tcggttgacg ctaactggcg ttcgacgttc
     4021 cagcagttac ctggtacggg agtcaaaccg gatcaattcc actctcaaac gcgtgaatat
     4081 ttccgccgcc tggcgaaaga cgcttcacgt tactcttcaa cgatctccga ccctgacacc
     4141 aatgtgaagc aggttaaagt cctgcagctc attaacgcat accgcttccg tggtcaccag
     4201 catgcgaatc tcgatccgct gggactgtgg cagcaagata aagtggccga tctggatccg
     4261 tctttccacg atctgaccga agcagacttc caggagacct tcaacgtcgg ttcatttgcc
     4321 agcggcaaag aaaccatgaa actcggcgag ctgctggaag ccctcaagca aacctactgt
     4381 ggcccgattg gtgccgagta tatgcacatt accagcaccg aagaaaaacg ctggatccaa
     4441 cagcgtatcg agtctggtcg cgcgactttc aatagcgaag agaaaaaacg cttcttaagc
     4501 gaactgaccg ccgctgaagg ccttgaacgt taccttggcg caaaattccc tggcgcaaaa
     4561 cgcttctcgc tggaaggcgg tgacgcgtta atcccgatgc ttaaagagat gatccgccac
     4621 gctggcaaca gcggcacccg cgaagtggtt ctcgggatgg cgcaccgtgg tcgtctgaac
     4681 gtgctggtga acgtgctggg taaaaaaccg caagacttgt tcgacgagtt tgccggtaaa
     4741 cataaagaac acctcggcac gggcgacgtg aaataccaca tgggcttctc gtctgacttc
     4801 cagaccgatg gcggcctggt gcacctggcg ctggcgttta acccgtctca ccttgagatt
     4861 gtaagcccgg tcgttatcgg ttctgttcgt gcccgtctgg acagacttga tgagccgagc
     4921 agcaacaaag tgctgccaat caccattcat ggtgacgccg cagtgaccgg gcagggcgta
     4981 gttcaggaaa ccctgaacat gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt
     5041 atcgttatca acaaccaggt tggcttcacc acctctaacc cgctggatgc ccgttcgacg
     5101 ccgtactgta ctgatatcgg taagatggtt caggcaccga ttttccacgt taatgcggat
     5161 gatccggaag ccgttgcctt tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa
     5221 cgtgatgtct tcatcgacct ggtatgctac cgccgtcacg gccacaacga agccgacgag
     5281 ccgagcgcaa cccagccgct gatgtatcag aaaatcaaaa aacatccgac gccgcgcaaa
     5341 atctacgctg acaagctgga gcaggaaaaa gtcgcgacgc tggaagatgc caccgagatg
     5401 gttaacctgt accgcgatgc gctggatgct ggcgattgcg ttgtagcaga gtggcgtccg
     5461 atgaacatgc actctttcac ctggtcgccg tacctcaacc acgaatggga cgaagagtac
     5521 ccgaacaaag ttgagatgaa gcgcctgcag gaactggcta aacgcatcag cacggtgccg
     5581 gaagcggttg aaatgcagtc tcgcgttgcc aaaatttatg gcgatcgcca ggcgatggca
     5641 gccggtgaga aactgttcga ctggggcggc gcggaaaacc tcgcttacgc cacgttggtt
     5701 gacgaaggca ttccggttcg cctgtcgggt gaagactccg gtcgcggtac cttcttccac
     5761 cgccacgcgg tgatccacaa ccagtctaac ggttccactt acacgccgct gcaacatatc
     5821 cataacggcc agggcgcgtt ccgcgtctgg gactctgtac tgtctgaaga agccgtactg
     5881 gcgtttgaat acggttatgc caccgcagaa ccacgcaccc tgaccatctg ggaagcacag
     5941 ttcggtgact tcgccaacgg tgcacaggtg gttatcgacc agttcatctc ctctggcgaa
     6001 cagaaatggg gccggatgtg tggcctggtg atgttgctgc cgcacggtta cgaagggcag
     6061 gggccggagc actcctccgc gcgtctggaa cgttatctgc aactttgcgc tgagcaaaac
     6121 atgcaggttt gcgtaccttc taccccggca caggtttacc acatgctgcg tcgtcaggcg
     6181 ttgcgcggga tgcgtcgtcc actggtcgtg atgtcgccga aatccctgct gcgtcatccg
     6241 ctggcggttt ccagcctcga agaactggcg aacggcacct tcctgccagc catcagtgaa
     6301 atcgacgagc ttgatccgaa gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat
     6361 tacgatctgc tggaacaacg tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc
     6421 gagcaactct acccgttccc gcataaagcg atgcaggaag tgttgcagca gtttgctcac
     6481 gtcaaggatt ttgtctggtg ccaggaagag ccgctcaacc agggcgcatg gtactgcagc
     6541 cagcatcatt tccgtgaagt gattccgttt ggggcttctc tgcgttatgc aggccgcccg
     6601 gcctccgcct ctccggcggt agggtatatg tccgttcacc agaaacagca acaagatctg
     6661 gttaatgacg cgctgaacgt cgaataaata aaggatacac aatgagtagc gtagatattc
     6721 tggtccctga cctgcctgaa tccgtagccg atgccaccgt cgcaacctgg cataaaaaac
     6781 ccggcgacgt agtcgtacgt gatgaagtgc tggtagaaat cgaaactgac aaagtggtac
     6841 tggaagtacc ggcatcagca gacggcattc tggatgcggt tctggaagat gaaggtacaa
     6901 ccgtaacgtc tcgtcagatc cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa
     6961 ccagcgccaa atctgaagag aaagcgtcca ctccggcgca acgccagcag gcgtctctgg
     7021 aagagcaaaa caacgatgcg ttaagcccgg cgatccgtcg cctgctggct gaacataacc
     7081 tcgacgccag cgccattaaa ggcacgggtg tgggtggtcg tctgacccgt gaagatgtgg
     7141 aaaaacatct ggcgaaagcc ccggcgaaag agtctgctcc ggcagcggct gctccggcgg
     7201 cgcaaccggc cctggctgca cgtagcgaaa aacgtgtgcc gatgactcgc ctgcgtaagc
     7261 gtgtggcaga gcgtctgctg gaagcgaaaa actccaccgc catgctgacc acgttcaacg
     7321 aagtcaacat gaagccgatt atggatctgc gtaagcagta cggtgaagcg tttgaaaaac
     7381 gccacggcat ccgtctgggc tttatgtcct tctacgtgaa agcggtggtt gaagccctga
     7441 aacgttaccc ggaagtgaat gcgtctatcg acggcgatga tgtggtctac cacaactatt
     7501 tcgacgtcag tatggcggtt tctacgccgc gcggcctggt gacgccggtt ctgcgtgatg
     7561 tcgataccct cggcatggca gacatcgaga agaaaattaa agagctggca gttaaaggcc
     7621 gtgacggcaa gctgacggtt gaagatctga ctggtggtaa ctttaccatc accaacggtg
     7681 gtgtgttcgg ttccctgatg tctacgccta tcatcaaccc gccgcagagc gcaattctgg
     7741 gtatgcacgc tatcaaagat cgtccgatgg cggtgaatgg ccaggttgag atcctgccga
     7801 tgatgtacct ggcgctgtcc tacgatcacc gtttgatcga tggtcgcgaa tccgtgggct
     7861 tcctggtaac gatcaaagag ttgctggaag atccgacgcg tctgctgctg gacgtgtagt
     7921 agtttaagct tcacctgcac tgtagaccga cgtggatagg gggtcggtct acggtttaaa
     7981 agataacgat tactgaagga tggacagaac acatgaactt acatgaatat caggcaaaac
     8041 aactttttgc ccgctatggc ttaccagcac cggtgggtta tgcctgtact actccacgcg
     8101 aagcagaaga agccgcttca aaaatcggtg ccggtccgtg ggtagtgaaa tgtcaggttc
     8161 acgctggtgg ccgcggtaaa gcgggcggtg tgaaagttgt aaacagcaaa gaagacatcc
     8221 gtgcttttgc agaaaactgg ctgggcaagc gtctggtaac gtatcaaaca gatgccaatg
     8281 gccaaccggt taaccagatt ctggttgaag cagcgaccga tatcgctaaa gagctgtatc
     8341 tcggtgccgt tgttgaccgt agttcccgtc gcgtggtctt tatggcctcc accgaaggcg
     8401 gcgtggaaat cgaaaaagtg gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg
     8461 atccgctgac tggcccgatg ccgtatcagg gacgcgagct ggcgtttaaa ctgggtctgg
     8521 aaggtaaact ggttcagcag ttcaccaaaa tcttcatggg cctggcgacc attttcctgg
     8581 agcgcgacct tgcgcttatc gaaatcaacc cgctggttat taccaaacag ggcgatctga
     8641 tttgcctcga cggcaaactg ggcgctgatg gcaacgcact gttccgccag cctgatctgc
     8701 gtgaaatgcg tgaccagtcg caggaagatc cgcgtgaagc acaggctgca cagtgggaac
     8761 tgaactacgt tgcgctggac ggtaacatcg gttgtatggt taacggcgca ggtctggcga
     8821 tgggtacgat ggacatcgtt aaactgcacg gcggcgaacc ggctaacttc ctggacgttg
     8881 gcggtggcgc aaccaaagaa cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca
     8941 aagtgaaagc cgttctggtt aacatcttcg gcggtatcgt tcgttgcgac ctgatcgctg
     9001 acggtatcat cggcgcggta gcagaagtgg gtgttaacgt accggtcgtg gtacgtctgg
     9061 aaggtaacaa cgccgaactc ggtgcgaaga aactggctga cagcggcctg aatattattg
     9121 cagcaaaagg tctgacggat gcagctcagc aggttgttgc cgcagtggag gggaaataat
     9181 gtccatttta attgataaaa acaccaaggt tatctgccag ggctttactg gtagccaggg
     9241 gactttccac tctgaacagg ccattgcata cggcactaaa atggttggcg gcgtaacccc
     9301 aggtaaaggc ggtactaccc acctcggcct gccggtgttt aacaccgtgc gtgaagctgt
     9361 tgctgccact ggcgctaccg cctctgttat ctacgtacca gcaccgttct gcaaagactc
     9421 cattctggaa gctatcgatg caggcatcaa actgattatc accatcactg aaggcatccc
     9481 gacgctggat atgctgacgg tgaaagtgaa actggatgaa gcaggcgttc gtatgatcgg
     9541 cccgaactgc ccaggcgtta tcaccccggg ggaatgcaaa atcggtatcc agccgggtca
     9601 cattcacaaa ccaggtaaag tgggtatcgt ttcccgttcc ggtacgctga cctatgaagc
     9661 ggttaaacag accacggatt acggtttcgg tcagtcgacc tgtgttggta tcggcggcga
     9721 cccgatccct ggctctaact ttatcgatat tctcgaaatg ttcgaaaaag atccgcagac
     9781 tgaagcgatc gtgatgatcg gtgagatcgg cggtagcgct gaagaagaag cggctgcgta
     9841 catcaaagag cacgtcacca agccagttgt gggttacatc gctggtgtga ctgcgccgaa
     9901 aggcaaacgt atgggccacg cgggtgccat cattgccggt ggtaaaggta ctgcggatga
     9961 gaaattcgct gctctggaag ccgcaggcgt gaaaaccgtt cgcagcctgg ctgatatcgg
    10021 tgaagcactg aaaactgttc tgaaataaat atctgtaata agaaatagcc ctcgccgctt
    10081 ccccctacag gaagggcgaa gggctgtcgg tttcgacatg gttggccatc ttatgatggc
    10141 cttttttatg cttatcgcgg cgattttcgc tgcgctatca gggtaaattt atagtcatca
    10201 gtattaaaag cgttgcggct atattcaaac acctggccat caactaaata tccacgcgat
    10261 actttttcaa gaatcggctt agtctggctg atattaagca gacggctcat ctcttccgtg
    10321 ggcatcaggg gaattatctc ctgttcgcta cgatcgataa ccatcttctt cacttcttca
    10381 ataaagtgat atttcgaatt ttccatgacc tgccaggtga gatccgggaa taatgcaagc
    10441 ggcatccagg tttcttccag cgccaccggc ttttgcttgc gatagcgcac gcgcttcaca
    10501 tgccacacac gatcctgctc gctaatttgc agctgctgct gaagaaaatt gtcagccgga
    10561 attacttcaa atatcaggac ttcactatgt gtttcgacgt gacggtccga cagtttttca
    10621 tcaaaactgg ttaactgaaa aatatcgtaa ttgacccgct cttctttgac gtaagtcccg
    10681 ctgccctgga tgctttcgag gatctgctgc tcgactaact ggcgcaaagc ctggcgcacc
    10741 gtaacccggc tgacgccaaa ctctgtttgt aatgccgatt cagtgggtaa cgcatcgcca
    10801 ggttttagct cgccgcacgt aatctgctca cgaatgcgat cggcaatctg ccggtataag
    10861 ggtttgtgtc ccatttctag tatctcatta atacgaattt agccattatg ctcgataaat
    10921 tcatcttgta aataataca
//
LOCUS       DACADA010000145        10862 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.147, whole genome shotgun sequence.
ACCESSION   DACADA010000145 DACADA010000000
VERSION     DACADA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.147"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            78..839
                     /locus_tag="ISB17_003780"
     CDS             78..839
                     /locus_tag="ISB17_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1981666.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            976..2313
                     /gene="adiC"
                     /locus_tag="ISB17_003781"
     CDS             976..2313
                     /gene="adiC"
                     /locus_tag="ISB17_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAP1981667.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2417..4060
                     /gene="eptA"
                     /locus_tag="ISB17_003782"
     CDS             2417..4060
                     /gene="eptA"
                     /locus_tag="ISB17_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAP1981668.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4057..4725
                     /gene="basR"
                     /locus_tag="ISB17_003783"
     CDS             4057..4725
                     /gene="basR"
                     /locus_tag="ISB17_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAP1981669.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4726..5826
                     /gene="pmrB"
                     /locus_tag="ISB17_003784"
     CDS             4726..5826
                     /gene="pmrB"
                     /locus_tag="ISB17_003784"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAP1981670.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5802..5891)
                     /gene="pmrR"
                     /locus_tag="ISB17_003785"
     CDS             complement(5802..5891)
                     /gene="pmrR"
                     /locus_tag="ISB17_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAP1981671.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6003..7505)
                     /gene="proP"
                     /locus_tag="ISB17_003786"
     CDS             complement(6003..7505)
                     /gene="proP"
                     /locus_tag="ISB17_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAP1981672.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7769..8647)
                     /locus_tag="ISB17_003787"
     CDS             complement(7769..8647)
                     /locus_tag="ISB17_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981673.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8644..>10862)
                     /gene="crfC"
                     /locus_tag="ISB17_003788"
     CDS             complement(8644..>10862)
                     /gene="crfC"
                     /locus_tag="ISB17_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAP1981674.1"
                     /translation="TLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLALPWSQ
                     PNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALP
                     TLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRI
                     ENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHL
                     AGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVR
                     EAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWG
                     YLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWED
                     SLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQN
                     IHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQDNSES
                     LSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGF
                     RPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPDWGRG
                     NEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFASCLTE
                     LQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2723 a         2858 c         2652 g         2629 t
ORIGIN      
        1 acaattatat aaccatttcc cggtatcgct tcgctttagc gagaactggt cttttattcg
       61 cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt ttattgactg
      121 aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa gctaaccata
      181 tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaat aacactttgg
      241 ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat aaagatctct
      301 cgcaaatacc agtatggcaa cgtagcgcta cgcccatcct cacccttcca tgcctgacgc
      361 cagacgtctt tcgcgttgcc gcgcaacata gcatgatgcc cgctgaaact gagtcagaaa
      421 aggaacgaac acgtgcatta ttattcactg tgctatcccg ttttctcgac agtaaaaaat
      481 tcctttcact aatgatgtat atgttacgta attgtgtaag tgacagcgtt tatcaaatta
      541 ttgaaagcga tatccacaaa gactggaatc ttagtatggt agccagttgt ttatgtctta
      601 gcccaagtct gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc caaataatca
      661 ccacctgccg catgcgctat gccgtaaatg aattaatgat ggacggtaaa aatatctctc
      721 aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc tttaaagact
      781 tctacggtat gacgcctctg cattatgtta gtcagcacag agaacgcact gtcgcctgat
      841 ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg gtcgataata
      901 attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga agctcgcctt
      961 cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt aatccccgtc
     1021 accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc tgcaaacctg
     1081 gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg tgcgctgggg
     1141 ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg ttcttacgct
     1201 tacgcccgcc gctgctttgg gccgtttctc ggttatcaaa ccaacgtcct ctactggctg
     1261 gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt aagttacttc
     1321 ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt gctgtggatc
     1381 ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtacaggc cgttgctacc
     1441 gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg gttccgtggt
     1501 gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc gattcaaagt
     1561 acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt tgccgcaggt
     1621 gtggtgaaaa acccgaaacg caacgtccct atcgccacca ttggcggggt attgattgct
     1681 gccgtttgct atgtactttc taccaccgcg attatgggga tgatccctaa tgccgcactg
     1741 cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg cgttgggtga caccgccggg
     1801 gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg ctggacgttg
     1861 ctggcaggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc gatttttgcc
     1921 cgtgtaaata aagcgggcac gccagtggcg gggctgatta tcgtcggtat tttgatgacc
     1981 atcttccagc tcagtagcat ttcgccaaac gcgaccaaag aattcggtct ggtttcttcc
     2041 gtgtcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt actgctgctc
     2101 ggccacggtc actttggtaa agcgcgcccg gcatacctgg cagttactac cattgccttc
     2161 ctctactgca tctgggccgt agtagggtcc ggcgcgaaag aggttatgtg gtcatttgtc
     2221 actctgatgg taatcaccgc catgtatgcg ctgaattaca accggctgca taaaaacccg
     2281 tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca aacataaaag
     2341 ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc gcactttgtt
     2401 cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga atttattctc
     2461 ctggttatta cttgctactt tttatatctc agtctgcctg aatatcgcct tttttaaaca
     2521 ggtgctacag gtgttaccgc tggattcact gcataacgtg ctggttttct tgtcgatgcc
     2581 ggtcgtcgca ttcagcgtga ttaatattgt cctgacacta ggctctttct tatggcttaa
     2641 tcgtccactc gcctgcctgt ttattctggt cggtgcggct gcgcaatatt tcatcatgac
     2701 ctatggcatc gtcatcgacc gttcaatgat caccaatatc attgatacta cgccggcgga
     2761 aagttatgcc ctgatgacgc cacgaatgtt attaacgctg gggttaagtg gcgtattagc
     2821 cgcgattatt gcctgctgga tcaaaatcaa acccaccacc tcacgcctgc gcagcgttct
     2881 tttccgcggg gccaacatcc tgatttctgt actgctgatt ctgctggttg ccgcactgtt
     2941 ctacaaagac tatgcctcgc tgtttcgcaa caacaaagaa ctggtgaaat ccttaagccc
     3001 gtccaacagc attgttgcta gctggtcgtg gtactctcac cagcggtcag ccaacctgcc
     3061 actgatacgc attggtgaag atgctcaccg caacccatta atgcaaaatg ggaaacgtaa
     3121 aaacctgacc attttgatcg tcggagaaac gtcgcgcgca gagaatttct cgctgaatgg
     3181 ttatccacgc gaaaccaatc ctcgtctggc aaaagataac gtggtctatt tccctaatac
     3241 cgcctcttgc ggcacggcaa cggcagtttc agtaccatgc atgttctcgg atatgccgcg
     3301 tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata tcattcagcg
     3361 agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggcg tttgcgatcg
     3421 cgtacctcac cagaacgtca ccgcgctgaa cctgcctggt cagtgcatca acggcgaatg
     3481 ctatgatgaa gtgctgttcc acgggctgga agattacatc aataacctgc agggtgatgg
     3541 cgtgattgtc ttacacacca tcggcagcca cggcccgacc tattacaacc gctatccgcc
     3601 gcagttcagg aaatttaccc caacctgcga cactaacgag atccagacct gtacccaaga
     3661 gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg ttgataaagc
     3721 gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt atctttctga
     3781 ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt atgccatcgc
     3841 cccggatagc caaaaacagg tgccgatgct gctgtggcta tcggaggatt atcaaaaacg
     3901 gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact attcacaaga
     3961 caatttattc tcaaccttat tgggcctgac tggcgttgag acgaagtatt accaggctgc
     4021 ggatgatatt ctgcaaactt gcaggagagt gagtgaatga aaattctgat tgttgaagac
     4081 gatacgctgt tattgcaggg attgattctg gcggcgcaaa ccgaaggcta cgcgtgcgat
     4141 ggcgtgagca ccgcgcggat ggcggaacaa agccttgaag ccggtcatta cagcctggtg
     4201 gtactggatt taggcttacc cgatgaagat gggctgcatt ttcttgcccg tatccggcag
     4261 aaaaaataca ctctgccggt gttaattctc accgcccgcg atacccttac cgacaaaatc
     4321 gccgggctgg atgtcggggc cgacgattat ctggtgaagc cttttgcgct ggaagagttg
     4381 cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag tgagctgatt
     4441 gttggcaacc tgacgctgaa tatgggtcgc cgtcaggtct ggatgggcgg tgaagagttg
     4501 attctgacgc ccaaagaata tgctctgctg tcgcgcctga tgcttaaagc aggcagcccg
     4561 gtgcatcggg aaattctcta caacgatatc tacaactggg acaacgagcc ctcgaccaac
     4621 accctggaag tgcatatcca taacctgcgc gacaaagtgg gcaaagcccg tatccgcacc
     4681 gtgcgcggat ttggctatat gctggtcgca aatgaggaaa actaattgaa tctgatgcgt
     4741 tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccatcgg ggccattttg
     4801 ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga gcagattcag
     4861 ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat gcgtgagatc
     4921 cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggttagcct gacgctattt
     4981 atctgctatc aggcggtacg ccgcatcacc cgaccgctgg cggagctgca aaaagagctg
     5041 gaagcgcgca ccgccgacaa cttaacgccc attgccattc atagcgccac cctcgaaatc
     5101 gatgcggtgg tttcggcgtt aaacgatctg gtcagtcgcc tgaccagcac gctcgataat
     5161 gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc gggggtgcgt
     5221 ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt agtggcacga
     5281 cttgatcaga tgatggagag cgtctcccag ttgctgcaac tggcgcgtgc cggacagtca
     5341 ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct cccctcgtat
     5401 gacgaactca gtaccatgct cgaccagcgc cagcaaaccc tgctattgcc agagagcgcc
     5461 gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg aaacctggta
     5521 gaaaacgccc atcgttatag cccgcaaggc agcaacatta tgattaagct gcaagaagat
     5581 ggcggagcgg tcatggccgt tgaagatgaa ggaccgggta ttgatgagag taaatgcggg
     5641 gagttgagta aagcatttgt acgaatggac agccgttatg gcggaattgg tctggggtta
     5701 agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca aaaccgacaa
     5761 gagacttccg gcacgcgggc ctggatacgg ctgaaaaaag atcagtacgt ggcaaaccag
     5821 atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact ttcataaaca
     5881 cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt cagagattaa
     5941 gtgaacctta agagttcaat tgacctgata agacagcatc acatcaggcc atccgtttca
     6001 gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc ctgcaaatcg
     6061 gcaatctcgt ggtcaatatc atcgattttc tgctcgatat tatcgtaatg ctcgacgaga
     6121 atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa cggacgattt
     6181 gccgtctctt tcatggttac gccggtgatt aaaccaacca ccgccactac catcaggtaa
     6241 taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag tgttggcgtc
     6301 agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta tcggatatgc
     6361 gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt aaggatcacc
     6421 gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat cagaataaac
     6481 gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg acgacggcca
     6541 aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat cataatggcg
     6601 ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa ataactcggc
     6661 atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc aatacatgtc
     6721 aacaggctgc gccagtgttt ggtggcaatc tctttaaacg agactttcgg gccatcctgc
     6781 aaaccttcgc ggtcgccctg ttccagttta tcgacatgct gctggaacgc cggagtctct
     6841 tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcgat aaagaacgga
     6901 atacgccagc cccaatcgag gaagttctct tcgccgacaa tggtcgaaat taacaccact
     6961 acgccagctc ccagcacaaa cccggcaata gaaccaaagt ccagccagct gcccataaag
     7021 ccacgtttac ggtcagggga gtattccgca acaaatattg acgccccggt atattcaccg
     7081 ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc ccaaatacca
     7141 atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat aatcacaata
     7201 gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa gaagagcccg
     7261 ccaagcggtc gaatcagaaa gggaacggag aaagtggcga gtgcagcaac catctgcacg
     7321 ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa accataaaca
     7381 ccaaaatcga accattccat tgcattaccc agtgatgctg cggtaatggc tttacgcagt
     7441 ttaccatcat caataatggt gacatcacga agggtaatcg gttttacttt tttccttttc
     7501 agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa tcagcgcgac
     7561 ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg tagcgggttt
     7621 gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga attgggcagt
     7681 taatgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac atgcatttcg
     7741 tttaggactc attggtgtaa ctgattattt acaccaaatt tacagagaag atttcctcga
     7801 ccattgcccc cgccagacgc tgcgctgaac agagtcgtgg catccccagt gcgacagttc
     7861 gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtagac cactttccca
     7921 gccaacttaa aaaatcagca tgatccccgc ccgtcgaaac ggtcgaagag gcaaaccact
     7981 gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaaa tgttgtgtcg
     8041 ctttcacgcg caaatcacct agcaacaacg ccaccgcttg cgggcttccc agacggcagg
     8101 catcgccaaa tgcaccccag cgcaactctt cgagaacgat aaaacagcgc ccaggcaacg
     8161 accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc tgttcacccg
     8221 cctgcaccaa taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa cgggctgaaa
     8281 accactcata caaggcgaca atttgctggg ggagctccgg gaaagcagag tgctggcggt
     8341 gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc agatgaccgg
     8401 gatccagcat cgacgccagt ttttccacca atgtcagacg caggctaagc ggcgcattta
     8461 acatgccgcc gttcgtccat gtctgaagct gcacgcgcgc aagaagttcc tgcatcagtc
     8521 gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc atcatcgccc tgcgccagat
     8581 ccactaaaaa gcgcggatga accgactcca gcacgcgacc ggggccgtcc agtaacgtct
     8641 tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca gctgagcgtc
     8701 ggtgtaaatc cagccgtgag tcatcacagt ctgctgcaat tgctgttgca tcagcctgac
     8761 caccgattca ttttgttgac gcagagccat actttcgcgt aaacgcgtct gtaattccgt
     8821 caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag attcatcgac
     8881 ctgctctgcc ataactttag ccatttgttg gcaataaaga tcgacttctg cgcttaatgc
     8941 tcgttgcaac acactgtaat caaccgtctc tgtcgggaat ttctcatttc cccgtcccca
     9001 gtcgggctga ttcaaccagc gcgaaaaaat ctcacgcaca acgcctaaac gcgtgctctg
     9061 ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcggg tgttgaagtg
     9121 gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaacccag actcactcaa
     9181 ttccattttg acttgttgct caatggggcg aataacatcg gttaacaccc ggcaaagggt
     9241 ggactcaagc tcggcaaagc gcagcgtaaa gtcgcgactg atggtgttct gcgccgtctg
     9301 taacagtgtc tcacagcggg tacgcatctc acttaacgac tccgaattat cctgaaacaa
     9361 cgcggctaac tgcgcattca ccgcatcttg ctgttgacgc agaaagtggt tggcggaggt
     9421 cagggccaac tcgatttcat gtttaatctc gccgctcacc tgcgcctgat tgagttgcaa
     9481 tagctgcaaa ctttcttcga cctgatggat attttgccgc aattgttcac aagcgacgtt
     9541 taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat tcaacagttt
     9601 atgcgcggca gatcgcagag catacaacga ggcgttagcg taagcggcat gaagcaacgc
     9661 ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat ggcgaatatg
     9721 ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat gggcaaaatc
     9781 ttccacccag cgttgttgct ctggcgcggg taacttaccg ttgttggcta actcatggcg
     9841 cgcccgattc gccaggtagc cccacatcga cgacaccgga aatatctgct gtggcgtaat
     9901 acagcctttc atcagcgttc cggaaatcag tgcccgcacc tggtcggcgt cgtcactgtt
     9961 acgatcctgt tgatcgaact tattgaccag cacatacaac ggcaccgatt gccccaccgc
    10021 caaaattgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat aatccagcac
    10081 cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca gatgcggttg
    10141 cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat aactctccag
    10201 ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg cggcgtaagc
    10261 agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc accaaatcat tcaaactttt
    10321 cagacaatga aaaataggtt gagcacccag ataatatttt tcgaaagcga tgccattttc
    10381 gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg tcagatgctt
    10441 aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt aaatggtcga ttggcgcgac
    10501 atgtgaaaaa tgcagtaccg gttctttttg cccgggcgta tggcgaataa gcgttggcag
    10561 cgcagtcatt gggcgattgc gattaggcag aacctccgta ccaacaatgg cattaatggt
    10621 ggttgatttc cctgctttca tggtaccgac aatcgccagc accatttcca gccgggaaat
    10681 tttacgcaac tcattattca gcatcgcgtg acgttcggcg atattaggct gactccaggg
    10741 taaagccagt tgtggcgcat cgtctccggg tacagagaga ggtatttttt ccagtaactg
    10801 caactgttgt cgggaaagct gtaacaggcg ttcagcctcc tgacttaact catacagggt
    10861 ct
//
LOCUS       DACADA010000146        10819 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.148, whole genome shotgun sequence.
ACCESSION   DACADA010000146 DACADA010000000
VERSION     DACADA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.148"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            44..769
                     /locus_tag="ISB17_003789"
     CDS             44..769
                     /locus_tag="ISB17_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1981675.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            727..1404
                     /gene="bglJ"
                     /locus_tag="ISB17_003790"
     CDS             727..1404
                     /gene="bglJ"
                     /locus_tag="ISB17_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAP1981676.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1442..2230)
                     /gene="fhuF"
                     /locus_tag="ISB17_003791"
     CDS             complement(1442..2230)
                     /gene="fhuF"
                     /locus_tag="ISB17_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAP1981677.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCDDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2371..2607
                     /locus_tag="ISB17_003792"
     CDS             2371..2607
                     /locus_tag="ISB17_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAP1981678.1"
                     /translation="MLQRTLGSGWGVLLPGLLITGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2646..2732)
                     /locus_tag="ISB17_003793"
     tRNA            complement(2646..2732)
                     /locus_tag="ISB17_003793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2696..2698),aa:Leu,seq:cag)
     gene            complement(2767..2853)
                     /locus_tag="ISB17_003794"
     tRNA            complement(2767..2853)
                     /locus_tag="ISB17_003794"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2817..2819),aa:Leu,seq:cag)
     gene            complement(2882..2968)
                     /locus_tag="ISB17_003795"
     tRNA            complement(2882..2968)
                     /locus_tag="ISB17_003795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2932..2934),aa:Leu,seq:cag)
     gene            complement(3168..4199)
                     /gene="rsmC"
                     /locus_tag="ISB17_003796"
     CDS             complement(3168..4199)
                     /gene="rsmC"
                     /locus_tag="ISB17_003796"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAP1981679.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLNRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4302..4715
                     /gene="holD"
                     /locus_tag="ISB17_003797"
     CDS             4302..4715
                     /gene="holD"
                     /locus_tag="ISB17_003797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAP1981680.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4684..5130
                     /gene="rimI"
                     /locus_tag="ISB17_003798"
     CDS             4684..5130
                     /gene="rimI"
                     /locus_tag="ISB17_003798"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP1981681.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5145..5822
                     /gene="yjjG"
                     /locus_tag="ISB17_003799"
     CDS             5145..5822
                     /gene="yjjG"
                     /locus_tag="ISB17_003799"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAP1981682.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5913..7502
                     /gene="prfC"
                     /locus_tag="ISB17_003800"
     CDS             5913..7502
                     /gene="prfC"
                     /locus_tag="ISB17_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP1981683.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7895..8500
                     /gene="osmY"
                     /locus_tag="ISB17_003801"
     CDS             7895..8500
                     /gene="osmY"
                     /locus_tag="ISB17_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAP1981684.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8627..8788
                     /locus_tag="ISB17_003802"
     CDS             8627..8788
                     /locus_tag="ISB17_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAP1981685.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8910..9983
                     /locus_tag="ISB17_003803"
     CDS             8910..9983
                     /locus_tag="ISB17_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAP1981686.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTCHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9980..10762
                     /locus_tag="ISB17_003804"
     CDS             9980..10762
                     /locus_tag="ISB17_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1981687.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2655 a         2700 c         2879 g         2585 t
ORIGIN      
        1 gaataaacca tcataatttt tagacagtgg atgtggagga aatatgttgc caggatgctg
       61 caaaaatgga attgttatca gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat
      121 gaggactcac ttccctgaat atgaaataat atccagcgcc tctgcggagg accttacctt
      181 attacaatta cgtcgttccg gattagtcat tgctgattta gccggtgaaa gtgaagatcc
      241 acgttctgtt tgtgaacatt attattcttt aatctcacaa tatcgggaaa ttcactgggt
      301 tttcatggtg tcacgctcct ggtattccca ggcagtagaa ctgctcatgt gccctacggc
      361 gacgttattg tctgatgttg aacccattga gaatctggtc aagaccgtac gttccggcaa
      421 tacgcacgca gagcgtatca gcgccatgct gacctctccg gcaatgactg aaactcatga
      481 ttttagctat cgctccgtca ttctcactct ttcagagcgc aaggtactgc ggctattagg
      541 taaaggatgg ggcatcaacc agatagcttc attgcttaag aaaagtaata aaactatcag
      601 cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc
      661 atggataaat agcgcgcagg gtgcaagaga acttaacttg ccttctgttt atggagatgc
      721 cgcagaatgg aacacagccg aattaagaag agaaatgtcg cactcataga aaaatgcgtc
      781 atgagtagta tcggtattga gagtttattc agaaagtttg cgggcaaccc ttataagctc
      841 catacctata ccagtcagga gtcatttcag gatgccatgt cgcggatctc gtttgcggcg
      901 gtcatttttt ctttttctgc catgagaagt gagcgcagag agggattatc ttgcctgact
      961 gaactggcga ttaagtttcc gcgtacccgg cgtttagtta ttgcggatga tgatattgaa
     1021 gctcggctga ttggttcatt gtcgccatca ccgctggacg gtgtattaag taaagcgtca
     1081 acgctggaga tttttcatca ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc
     1141 gaccgactga acaatcagtg gtacattaac caaagccgga cgctaagccc gacggagaga
     1201 gaaatattgc gctttatgtc gcgtggctac tcaatgacac aaattgccga gcagcttaaa
     1261 cgcaatatca aaacgatccg cgcgcataaa tttaatgtga tgtcgaaact gggcgtcagt
     1321 tctgatgcag gattgttgga ggccgcagat attctgttat gtatgcggca ttgcgaagca
     1381 agtaatgtgt tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct
     1441 ttcatttcag cgtacaatcg ccacattgct gcacatccgg taagcgataa cgctggcagc
     1501 aagtgcggcg caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt
     1561 caccgttcgt gagcgttttc tcaaaaaaga tggcatggcg cagcgattca accgtcgcct
     1621 cgccgagcag ttgcttcatc tcagtgagat accagttgat caaataaccg gtattactcc
     1681 agataagttt gccgttgatc tctccggtcg cttctagtgc ctgcacaacc ggaaccagcg
     1741 cctggctgat taacgtttcc attcgctgct gcggcgaatg tggtgttgcg tttttatcgt
     1801 cacacacatc gacccagaaa caggcggcac ggccggtttc gtgaaactca gcatggaaat
     1861 gttccggcga cacatctaat gctttttcct gcgtcagtag cgccagcatt aatggtggca
     1921 ccatcaggcc gatataccat tgtgcccata gtgagatcag cggtttgttc tcgcggatca
     1981 tcgttggttg gttgcgatag atatgatcgg aatagaccgc gagtagagaa cttagcgcat
     2041 tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg agcgggttca tccaggcgga
     2101 taaactcgag caaatgttca cgatgtttgg cgatcgtcgc acgtacggct tgtgcaagcc
     2161 cagcatcctg tggctggaga tgcgttcgcc agataacatc ttcatagagc ggtgcggaac
     2221 gataggccat aatcgggata gtaatctaaa tgataatgat tgctaatcct agcgataggt
     2281 ttatccgata gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg
     2341 gttatcatta tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg
     2401 ggagtgttgc tgccgggatt gctgattaca gggctgatgt atgcggattt atcgccggat
     2461 caatggcgga ttgtcattct gatgggattg gtattgacgc cggtgatgct gtatcacaaa
     2521 cagttacggc attacgtttt gctaccatcg tgcctggcgc ttattgctgg catcatgctg
     2581 atgataatga atttgaatca ggggtgataa aacagtgaag agacaagaaa agaagtaaag
     2641 ataattggtg cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa
     2701 gctagcgcgt ctaccaattc cgccaccttc gcacagtcat cttacttttt ttgatattgc
     2761 ctcgtttggt gcgagggggg ggacttgaac ccccacgtcc gtaagaacac taacacctga
     2821 agctagcgcg tctaccaatt ccgccacctt cgcccagtgc gagcaatatc aacgtggttt
     2881 ttggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
     2941 gcgcgtctac caattccgcc accttcgcat accatcaatt cttaaaaaga attgctacca
     3001 cggaggcgca ttctagtggt tttcagcttt tcgtcaatag ttaattatcg acagaggtgt
     3061 aattgctgga aaaatgtcca tcagcggatt gtggataatt cgtttgtcgg tcagataagg
     3121 cgtgtacgcc gcatccaaca cggtatgcgg cgacagatat cagatactta acctttcttc
     3181 gcctggcggg tcataatggc gcgatacacc ttgaagcgcc cggtttgcgc aatcacttcg
     3241 tggaaaccaa atgtctcatc cagcacgtcc gggtaaggca ggaaggcgtt cgctacaatt
     3301 cgcagctcgc cgccgctatt aagatgacgc accgcgccgc gaatcagcgt ttgcgccgca
     3361 tccaggctgg tttgcatccc atcgtggaac ggcgggttgg agatgatcat atcaaaacga
     3421 cctttcacct cggaaaagac gttgctggca aagacttcac cttcaacact gttggcagca
     3481 agtgttgcgc gactggcttc taccgctggt gcagaaacat cacacagagt gaggcggatc
     3541 ttcggtgaat ggcgcgcaaa agcaacggaa agaacgcctg cgccgcagcc gacatccagc
     3601 actttacctt tcgtgtgcgg ggttagcgtc gagagcaaca actgactgcc gacatccaga
     3661 ccgtcgcggc taaacacgcc cggcagcgtt ttgaccgtca agccatcgac gctgtattcg
     3721 ccccagaatt tattggcatc aaataccggc tgcttttcca gacgaccaaa atagaggccg
     3781 cagcgacggg cgctgtctac cttattcaac ggcgcataat cggccagcat ctgctcggcg
     3841 ctgcgcacgc cgctgcggtt ctcgccaacg acaaaaatat ccgtgcccac cggaagcaga
     3901 gaaagtaaat tcatcaactg gaactgggct tccggtttat tcttcggcca gtagtaaatc
     3961 aaagtatcgc aatcagcgac atcatccgcc gtggcgacca ggctaaaacg ggcgttatcc
     4021 cccatctggc ggtttaacac ctgccagtgg tggaattgct gggtatgagc acggctggcc
     4081 gcggtatcta aacgcgcggg caggtcatcc tgtaagtctc cggcaaacag aatacggctt
     4141 tgttcgaaat catcactgtg acgcagcaag acttcacttg ccggggtaaa tgcagacatg
     4201 gaatgctcct caattgatac tggcggcgat tatagccata tgttggcgcg gtatcgacga
     4261 atttgctata tttgcgcccc tgacaacagg agcgattcgc tatgacatcc cgacgagact
     4321 ggcagttaca gcaactgggc attacccagt ggtcgctgcg tcgccctggc gcgttgcagg
     4381 gggagattgc cattgcgatc ccgccacacg tccgtctggt gatggtggca aacgatcttc
     4441 ccgccctgac cgatcctctg gtgagcgatg ttctgcgtgc gttaaccgtc agccccgatc
     4501 aagtgctgca actgacgcca gaaaaaatcg cgatgctgcc gcaaggcagc cgctgcaaca
     4561 gctggcggtt gggtactgac gaaccgctat cactggaagg cgctcaggtg acatcaccgg
     4621 cgctcaccga attacgggca aacccaacgg cacgcgccgc gttatggcaa caaatttgca
     4681 catatgaaca cgatttcttc cctcgaaacg actgatttac cagcggcttt ccacattgaa
     4741 caacgcgccc acacctttcc gtggagtgaa aaaacctttg ccagcaacca gggcgagcgc
     4801 tatctcaact ttcagttaac gcaaaacggc aaaatggcgg cgtttgcgat tacgcaagtg
     4861 gtgctggatg aagctacatt gttcaatatt gcggtcgatc ctgactatca gcgtcaggga
     4921 ctgggaaggg cgctgctgga acatctgatc gacgaactgg aaaaacgcgg cgtggcgacg
     4981 ctatggctgg aagtccgtgc ctcaaatgct gccgccattg ccctgtacga aagtttaggc
     5041 tttaacgagg cgacgattcg ccgcaattac taccccacca cggacggtcg cgaagacgcc
     5101 atcatcatgg cgttaccaat cagtatgtaa gacaaggtgg aataatgaag tgggactgga
     5161 ttttctttga tgccgatgaa acgctgttta cctttgactc attcaccggc ctgcagcgga
     5221 tgtttcttga ttacagcgtc acctttaccg ctgaagattt tcaggactat caggccgtta
     5281 acaagccact gtgggtggat tatcaaaacg gcgtgatcac ttcattacag cttcagcacg
     5341 ggcggtttga gagctgggcc gaacggctga acgtcgaacc gggtaaactc aacgaggcct
     5401 ttattaatgc gatggcggaa atctgtacgc cgttgccggg ggcggtttcc ctgcttaacg
     5461 ccattcgtgg caacgctaaa atcggcatca tcaccaacgg ctttagcgcc ttgcagcaag
     5521 tgcgtctgga acgcacgggc ctgcgtgatt acttcgattt gctggtgatt tccgaagaag
     5581 ttggcgttgc caaaccgaat aagaaaattt tcgattatgc gctggaacag gcgggcaatc
     5641 ctgaccgttc acgcgtgctg atggttggcg acactgccga gtccgatatt ctcggtggca
     5701 tcaacgccgg gcttgcgacc tgctggctga atgcgcacca tcgcgagcaa ccagaaggca
     5761 tcgcgcccac ctggaccgtt tcttcgttgc acgaactgga gcagctcctg tgtaaacact
     5821 gattgcctcc cccccgttga tgggtaaaat agccgcaatt tttcgttttc aacaagcgcg
     5881 gcgcgatgcc gcttactcaa gaagaaagaa ttatgacgtt gtctccttat ttgcaagagg
     5941 tggcgaagcg ccgcactttt gccattattt ctcacccgga cgccggtaag actaccatca
     6001 ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc cggtacagta aaaggccgtg
     6061 gttccaacca gcacgctaag tcggactgga tggagatgga aaagcagcgt gggatctcca
     6121 ttactacgtc tgtgatgcag tttccgtatc acgattgcct ggttaacctg ctcgacaccc
     6181 cggggcacga agacttctcg gaagatacct atcgtaccct gacggcggtg gactgctgtc
     6241 tgatggttat cgacgccgca aaaggtgttg aagatcgtac ccgcaagctg atggaagtta
     6301 cccgtctgcg cgacacgccg atcctcacct ttatgaacaa acttgaccgt gatatccgcg
     6361 acccgatgga actgctcgat gaagttgaga acgagctgaa aatcggctgc gcaccgatca
     6421 cctggccgat cggctgcggc aagctgttta aaggcgttta ccacctttat aaagacgaaa
     6481 cctatctcta tcagagcggt aaaggccaca ccattcagga agtccgcatt gttaaagggc
     6541 tgaataaccc ggatctcgat gcagcggtcg gtgaagatct ggcacagcag ctgcgtgacg
     6601 aactggaact ggtgaaaggc gcgtctaatg agttcgacaa agagctgttc cttgcgggcg
     6661 aaatcactcc ggtattcttc ggtactgcgc tgggtaactt cggtgtcgat catatgctgg
     6721 atggcctggt ggagtgggct cctgcgccga tgccgcgtca gactgatacc cgtaccgtag
     6781 aggcgagcga agacaaattt accggcttcg tatttaaaat tcaggccaac atggacccga
     6841 aacaccgcga ccgcgtggcg tttatgcgcg tggtatccgg taaatatgaa aaaggcatga
     6901 agctgcgcca ggtgcgcact gcgaaagatg tggtgatctc cgacgcgctg acctttatgg
     6961 caggtgaccg ttcgcacgtt gaagaagcat atccgggcga tatcctcggc ctgcacaacc
     7021 acggcaccat tcagattggc gacaccttta cccagggtga gatgatgaag ttcaccggta
     7081 ttccgaactt cgcgccagaa ctgttccgtc gcatccgcct gaaagatccg ctgaagcaaa
     7141 aacagctgct caaagggctg gtacagcttt ccgaagaggg cgcggtgcag gtgttccgtc
     7201 caatctccaa caacgatctg atcgttggtg cggttggtgt gctgcagttt gatgtggtgg
     7261 tatcgcgcct gaagagcgaa tacaacgttg aagcagtata tgaatcagtc aacgttgcca
     7321 ccgcccgctg ggtagaatgt gcagacgcga agaaattcga agagttcaag cgtaagaacg
     7381 aaagccaact ggcgcttgat ggcggcgata acctcgctta catcgctacc agcatggtca
     7441 acctgcgcct ggcgcaggaa cgttatccgg acgttcagtt ccaccagacc cgcgagcatt
     7501 aattcttgcc tccagggcgc ggtagccgct gcgccctgtc aatttccctt ccttattagc
     7561 cgcttacgga atgttcttaa aacattcact tttgcttatg ttttcgatga tattccgagc
     7621 ggtttcaaaa ttgtgatcta tatttaacaa agtaatgaca tttctgacgg cgttaaatac
     7681 cgttcaatgc gtagatatca gtatctaaag ccgtagattg tcattctacc gatattaata
     7741 actgattcag aggctgtaat ggtcgttatt catcacccat cgcttttgtg atggcgacca
     7801 ttgacttctg tagagggtga agtctctccc tattcagcaa tgcaacctcg tgttgccagg
     7861 ctcaaattac gagcaaactt acaggaataa atcgatgact atgacaagac tgaagatttc
     7921 gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg acaggctctg cctacgcgga
     7981 aaacaacgcg cagactacca atgaaagcgc agggcaaaaa gtcgatagct ctatgaataa
     8041 agtcggtaat ttcatggatg acagcgccat caccgcgaaa gtgaaggcgg ccctggtgga
     8101 tcatgacaac atcaagagca ccgatatctc tgtcaaaacc gatcaaaaag tcgtgaccct
     8161 gagcggtttc gttgaaagcc aggctcaggc cgaagaggca gtgaaagtgg cgaaaggcgt
     8221 tgaaggggtg acctctgtca gcgacaaact gcacgttcgc gacgctaaag aaggctctgt
     8281 gaagggctac gcgggtgaca ccgccaccac cagtgaaatc aaagccaaac tgctggcgga
     8341 cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc gacggcgtgg ttcagctctc
     8401 cggtaccgtc gattcccagg cacagagcga ccgtgctgaa agtatcgcca aagcggtaga
     8461 tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa ttcgtcgtaa ttcgtcctcc
     8521 cgaactttgt cgggaggcgc gatgtgcacc acactaaaaa tatcgcattg agtgattttc
     8581 acgctcatat caagcggtga cattaactat ggtaaaggag acgcttatgt ttcgttgggg
     8641 catcatattt ctggttatcg cgttaatcgc cgccgcactt gggtttggtg gtctggccgg
     8701 taccgctgca ggcgcagcta aaattgtctt tgtcgtcggg attattctgt tcctggtgag
     8761 tttgttcatg ggccgaaaac gaccctagat ttcagatcat taagaaaatt ttatccaaag
     8821 ccagtccagc ggactggctt ttgcggtttt agcgaataat aaattgcgtt actttgtcat
     8881 tctttcacaa caaaaacagg aaggcagagg tggggcagcg aatacctgta acgcttggta
     8941 atattgcgcc gttgtcgcta aggccgttcc agcctggacg aatagctctg gtgtgcgaag
     9001 gcggcggaca gcgtggaatt ttcacggctg gcgtgctgga tgagtttatg cgcgcgcagt
     9061 ttaatccttt cgatctttat ctcggcacat ctgccggggc gcagaacctc tcggcgttta
     9121 tctgcaatca gcccggttac gcgcgcaaag tcatcatgcg ctataccaca aaacgcgaat
     9181 ttttcgatcc attgcgcttt gtccgtggag gaaatcttat cgatctcgac tggctggtgg
     9241 aggccaccgc aagccagatg ccgttgcaaa tggataccgc cgcgcggttg tttgacagcg
     9301 gcaaatcgtt ttatatgtgc gcctgtcgtc aggatgacta cgcgcctaat tactttttac
     9361 caaccaaaca aaactggctg gatgtgattc gcgcctccag tgcgatacct ggcttttatc
     9421 gtagcggagt gtcgctggaa ggcattaact acctggatgg cgggatcagt gatgcgattc
     9481 cggttaaaga ggcggcaagg cagggcgcta aaacgttggt cgtcatccgc actgtgccgt
     9541 cacaaatgta ctacacgccg cagtggttca aacgcatgga acgctggctg ggtgacagta
     9601 gcctgcagcc gctggtcaat ctggtgcagc atcatgaaac cagctatcgt gacattcagc
     9661 aatttattga gaaaccaccg ggcaagctgc ggatattcga aatttatcca ccgaagccat
     9721 tacatagtat cgcgcttggc agtcggattc cggcgctgcg tgaagactat aaacttgggc
     9781 gtttatgcgg tcgttatttc ctcgccacgg ttggcaagct attaactgaa aaagcgccgc
     9841 ttacctgcca tctggtgcca gtggtgacgc cggaatcgat cgttattccg cctgcgccag
     9901 tcgccaacga tacgctggtt gccgaagtga gcgatgctcc gcaggcgaac gacccgacat
     9961 ttaacaatga ggatctggct tgatttgccg ttttatcgat acccactgcc attttgattt
    10021 cccgccgttt agtggcgatg aagaggccag cctgcaacgc gcggcacaag cgggcgtagg
    10081 caagatcatt gttccggcga cagaggcgga aaattttgcc cgcgtgcagg cgttagcgga
    10141 aaaatatcaa ccgctgtatg ccgcattggg gttgcatccg gggatgttgg aaaaacatag
    10201 cgatgtgtct cttgatcagc tacagcaggc gctggaaagg cgtccggcga aggtggtggc
    10261 ggtgggggag atcggtctgg atctcttcgg cgacgatccg caatttgaga ggcagcagtg
    10321 gttactcgac gaacagctga aactggcgaa acgctacgat ctgccggtga tccttcattc
    10381 ccggcgtaca cacgacaaac tggcgatgca tcttaaacgt cacgatttac cgtgcactgg
    10441 cgtggttcac ggtttttccg gcagcctgca acaggccgag cggtttgtac agctgggcta
    10501 caaaatcggc gtaggcggta ctatcaccta tccacgcgcc agtaaaaccc gcgatgtcat
    10561 cgcaaaatta ccgctggcat cgttgttgct ggaaaccgac gcgccggata tgccgctcaa
    10621 cggctttcag gggcagccta accgaccgga acaggctgtg cgggtgtttg acgtgctttg
    10681 cgagttgcgc ccggaaccag aagatgagat tgccgaagtg ttgcttaata atacgtatgc
    10741 gttgtttagc gttagtgggt agtttaccct tcgtaggcag catccggcag tcggcgcata
    10801 atgcctgatg cgacgcttg
//
LOCUS       DACADA010000147        10687 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.149, whole genome shotgun sequence.
ACCESSION   DACADA010000147 DACADA010000000
VERSION     DACADA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.149"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            14..1702
                     /locus_tag="ISB17_003805"
     CDS             14..1702
                     /locus_tag="ISB17_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAP1981688.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGIPV
                     AERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            1699..2613
                     /locus_tag="ISB17_003806"
     CDS             1699..2613
                     /locus_tag="ISB17_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP1981689.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2645..2893
                     /locus_tag="ISB17_003807"
     CDS             2645..2893
                     /locus_tag="ISB17_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP1981690.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2893..4065
                     /locus_tag="ISB17_003808"
     CDS             2893..4065
                     /locus_tag="ISB17_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP1981691.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4100..5179)
                     /gene="lptG"
                     /locus_tag="ISB17_003809"
     CDS             complement(4100..5179)
                     /gene="lptG"
                     /locus_tag="ISB17_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP1981692.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5176..6246)
                     /gene="lptF"
                     /locus_tag="ISB17_003810"
     CDS             complement(5176..6246)
                     /gene="lptF"
                     /locus_tag="ISB17_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP1981693.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6277..6837)
                     /locus_tag="ISB17_003811"
     CDS             complement(6277..6837)
                     /locus_tag="ISB17_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP1981694.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6849..7673)
                     /locus_tag="ISB17_003812"
     CDS             complement(6849..7673)
                     /locus_tag="ISB17_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981695.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(7673..9022)
                     /locus_tag="ISB17_003813"
     CDS             complement(7673..9022)
                     /locus_tag="ISB17_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP1981696.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9068..9841)
                     /locus_tag="ISB17_003814"
     CDS             complement(9068..9841)
                     /locus_tag="ISB17_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981697.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSPA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9873..10586)
                     /locus_tag="ISB17_003815"
     CDS             complement(9873..10586)
                     /locus_tag="ISB17_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAP1981698.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
BASE COUNT         2645 a         2796 c         2672 g         2574 t
ORIGIN      
        1 gcattcccta cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactatgc
       61 cgctctgagt agcgctggaa tgaattttta tgacagacgt tgccaacttg aagatcaact
      121 ggaatatcag acgttaaaag cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa
      181 cctgaaaaaa ggcgatcgcg tggcactgat tgccgaaacg agtagcgggt ttgtagaggc
      241 tttttttgcc tgccagtatg ccggcttagt cgccgtcccg ttggcgattc caatgggcgt
      301 cggtcagcgg gattcctgga gcgccaaact gcaggggtta ctggcaagtt gccagcccgc
      361 agccattatc actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaatcc
      421 cgaattacat gttttaagcc acgcctggtt taaggcatta ccggaagccg atgttgcgct
      481 tcaacgtcca gttccaaacg atatcgccta cctccagtac acctccggca gcacccgttt
      541 tccccgtggc gttatcatta ctcaccgcga agtgatggct aatctacgtg ctataagcca
      601 cgatgggatt aaattacgcc ctggcgaccg ctgcgtctcc tggctgcctt tctaccatga
      661 tatgggactg gtcggctttc tcctgacccc cgtcgccacg cagctttcag tagattattt
      721 gcgcactcag gattttgcca tgcgtcctct gcaatggctt aaattgatca gtaaaaatcg
      781 cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg tgccagcgcc gcgtgaatga
      841 aaaagatctc gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgcagaacc
      901 catctccgca gaacaactcc atcaattcgc tgaatgtttc cgtcaggtca actttgacga
      961 taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga
     1021 tgaagcttcc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat accagggtaa
     1081 agccgtcgcg ccgggtgctg aaacacgcgc cgtatcgact ttcgtcaact gcggtaaagc
     1141 gttgccggaa catggcattg aaatccgcaa tgaagcaggt atacctgtcg cggaacgtgt
     1201 ggtaggccat atttgcatct ccggccctag cctgatgagc ggttactttg gcgaccagat
     1261 ttcacaagac gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct
     1321 ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa
     1381 tatctggccg caggatattg aatatattgc ggaacaggaa ccggaaattc attctggcga
     1441 tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag
     1501 cgatgaagaa cgtcgcgggc agcttatcca cgcgctggca gcacggatcc aaagcgaatt
     1561 tggcgttacc gcggatatcg atctgttgcc gccccacagt attccccgaa catcctccgg
     1621 caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttca
     1681 tgtgcaggag tccctggcat gaatcaaact gtcgcggtga cgggcgctac cgggtttatc
     1741 ggtaaatata ttattgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt
     1801 actgcccgcg ctcacgtcaa tgataatctc acttgggtgc gcggttcgct ggaagataca
     1861 cattcgctta gcgaactggt taccggagcc agcgcggtgg tccattgcgc cgggcaagtg
     1921 cgcgggcaca aagaagagat ttttacccgc tgcaacgttg acggcagcct gcgcctgatg
     1981 caagcggcaa aagagagcgg cttttgccaa cgatttctgt ttatctcttc gctggcggcg
     2041 cgccatcccg aactctcctg gtacgcaaat tccaaacacg tcgccgaaca acagctgact
     2101 gcaatggctg acgcaattac gttgggcgtt tttcgcccga cagccgtgta tggtcccggc
     2161 gataaagagt taaaaccgct gtttgactgg atgctgcgcg gtctgctgcc acgacttggc
     2221 acgccagaga cacagctctc tttcctgcac gtcaccgatt ttgcgcaagc agtgggtcag
     2281 tggttaagcg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccgga
     2341 ggctatgact ggcaacgtgt ccaacagctt gttgccgatg tccgctgtgg ttccgtgcgg
     2401 atggtcggta ttcctctgcc gctactcacc tgccttgcgg atatcagtac cgcgttgagc
     2461 cgtctggcgg gtaaagaacc gatgctgact cgttcgaaaa tccgtgaatt aacccatgcc
     2521 gactggtcgg caagtaataa ccgtatttct gaagatatta attggtttcc cgggattagc
     2581 ctggaacacg cattacgcaa cgggctattt tgattgtctt gtttaaaggt gtgttaacgt
     2641 ttctatggta aatcgtgaaa tagtaatgga ttacatcctt tcctgtttac aggatttggt
     2701 cgaaaacggt gtggaaatta agcccgacag cgatctcgtc aacgatcttg gcctggagtc
     2761 gatcaaagtg atggatctct taatgatgct ggaagacaga ttcgatattt ccattcctat
     2821 taatattctg ctggatgtca aaacccctgc gcaattaatg gaaactctac ttccatggct
     2881 ggagaataaa taatggggct atacgataaa tatgcgcgcc ttgctggcga gcgactgcaa
     2941 tttagtgaca acgggttaac accgtttggc acctgtattg atgaagtgta ttccgccacc
     3001 gaaggtcgga tcggcaataa aaaagtgatt ctggccggga ccaataatta tctcggttta
     3061 acctttaatc atgatgccat cgccgaaggt caggcagcgt tagcggccca aggcaccggc
     3121 accaccggtt ctcgtatggc aaacggcagt tatgcgccgc acctggcgtt agagaaagag
     3181 attgccgagt ttttcaatcg ccctaccgct atcgtttttt caaccggtta taccgctaat
     3241 ttaggcgtca tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc
     3301 cacgccagta tttatgacgc ctgttctctc ggtggcgcag aaattattcg tttccgccat
     3361 aacgatgcta aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg
     3421 attatcatcg ttgagggcat ctacagcatg ctcggtgacg ttgcgccgtt agcggaaatt
     3481 gttgatatca aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc
     3541 gtgttaggcg caacggggcg cggactggct gaagccgttg gcgttgaaga tgatgtggat
     3601 attatcgttg gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcggttcg
     3661 gaagcgatgg aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct
     3721 ccgtcctgca ttgcgaccgt gcgctcgtcg ttaagaacca ttgccacaca accagagctg
     3781 cggcaaaaac tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat
     3841 gagttaagtt cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt
     3901 ttacgcatct ggcgcgagct tatttcgctg ggcgtttatg tgaatcttat cctgccacct
     3961 gccgcgcctg caggcatcac tctgctgcgc tgtagcgtaa acgctgccca cagtcgtgag
     4021 caaatcgatg ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgctgtg
     4081 gtgttgcgcc acggcgggtt taatgttgtc tgtcatacca atacaccagg ctcaacgcca
     4141 ccaacacaaa cgctaccggt aaaccgagcg cggtaacagg tgcgctcaac gcaaataaca
     4201 atcccagatt gaccatgatt tgatagctga tccaggtgag taagccaacg atgacgccta
     4261 cagcgagacg gctacccatc cctggcgagc gcggggcgct aaaggtgaac ggcacagcca
     4321 gcaaaatcat cgccagggtc aggatcggtt gccccagttt ttcccacagt gcaaggcggt
     4381 attcgctgct gggttgtccg gtttcctgca aataatggat gtaatgatta agctgacgga
     4441 cagaaaaagt attccccggc atcgataact cttcaagatc catactggtg aaggccgatt
     4501 gccaggcaag attatctgat gtttccagcg tttctttgcc attcaaccat tttttatgat
     4561 tcacaccatg caggatccac gttttgtcgt cttcaatgga ggcactgcgt gcaaaaatgt
     4621 aggattcgag cgagagatcg tcgcgataat gaaatatctc cacgcccaca ggctggccct
     4681 gctcgttcag cgatttcacc gtcacaaatt cattaccgcg cctggcccaa agcatattgc
     4741 cggtaatgtc atcgtcctcc cccaacgcgg tggcagtcga tttgatttgc agcgcctgtt
     4801 gctgcaatgg cgacgccacc cactcatcta tcgcgcctaa cgaaacagtc cacaatatcc
     4861 cggcaaccag cgccaccagt gcaatacgga agatagaaaa cccagtgctg cgaatggcgg
     4921 taagctcact gtttttcgat aactgcccca ggccaacaat ccctcccaat aatgcgataa
     4981 acggcgaaag ttcgaccagt gtacgcggta aggtcatcag caccaccagc accgcctgag
     5041 tccagcgata accgcccggg ctgacatcat ccagttcgtt aatcaggttg aaggtggtaa
     5101 aaagcggcag caatagccct gcggcggcgg caaaaccgag aaagagatga cggattaaat
     5161 agcgactgaa aacattcatc gggaaaattt ctgcagcaaa gagaagtcgc gtgcgaccag
     5221 catcagcagg cccattagca tgagtccggg tactaaccac aaaccaggaa tgaggggaat
     5281 cgcaccgtta gccaccagcg tacggcagac gtcgccgcca taaaaaatgg caacaaacag
     5341 caatgtcagt ggcaataacg ttgaaaatcg cccttgccgc ggttttaccc gacttaatga
     5401 aatggccagc agcgccatca acaatgctgt caggccacgg ctttgtcgcc attgcagttc
     5461 ggcatgatcg gcaggaaagg ctgagcgcgc cagctccgtg accgattttg ctttgcgttt
     5521 gacgttaggg ctttgatcca gcggattcag atgtaattgc agattacggt agatctgttc
     5581 gttgtcgtca cgcccctgat gatcgagaag ataggcggtc ccgttatgca acataacggt
     5641 cggtttctca ggtgatgggt caaccacatc aaccgaacgg gcgcggaaaa tgcgggttcg
     5701 attggcagta gaagtataaa tcagcgcgtc agtcaggcga ttattatcct gatcaaccgt
     5761 ctgcgaaagg atcattcgtc cgttatcgtt agtgttaaat ttctttgccc gcaactggcg
     5821 aacatccagc tccgactgcg actgttgctc cagttgataa atctgcgcat aagcccacgg
     5881 ccgaccatac atcgacagaa gggtgacaaa aatacttagc ggtatcgcca gataaagaac
     5941 ggctttgtac aagcgtcccg gattgccccc cgccgcagag atagcggtaa tttccgagtc
     6001 ggtatacatc tgccctagcg tcacgccaac tgacacatac agaccaacag gtaacaacat
     6061 ctccagtgca atcagcactt tgtagaaaac gatatccagc acaacatcaa gagctaaggt
     6121 cccatttgcc gcttcggtca gataacgctg tgcggagtag ctggcgaaaa taaagatcag
     6181 aaaaccgacg ataatcagca ccagacggcg cgtaccacgc atgatgtagt gttcaaccag
     6241 cttcatctca ctaacaccct caatcctttg aattttttat tcattgatca gtttctgcgc
     6301 cagcgcaatg acatgatcta cctcttccgg cgtcaggctg ccgtcctttg cccactgaat
     6361 ttgcccgtct ttattcagca ccagaatggt tgaactctgc tccggcaacc gccaggcaac
     6421 gcgtcccagt ccattgccgt caataacgaa ttgcgcccac ggatagcggc gtttattttt
     6481 ttcgattttg ccaacgacaa aatagccagt accaaagatg gcatcatcgg tgttcacaat
     6541 ggtggtgggc tgaaaacgat cctgcggaaa gtttgccgct tcaaccgctt taattaacag
     6601 tgagttcttt ttcttcgccg acttacgccc ggcgatatac tggataatgc ggacttttcc
     6661 cgccagctgt gtgctgttcc acttttggta gtgaatttcg tcgtcgctat tcagaagcag
     6721 ctcgccgcct tcctggatat aaacgggggt aaccgttttg ccagtgacaa aattgtgtgc
     6781 ctgcacaccc tgggctaaga gcatgataat cagcgcaata atcaaacgag atgacatcat
     6841 tgctccggtt atagcggaaa acgcgaaatc agcagctcta agcgctggcg aatatcgcta
     6901 tccagcgcca ccggtgcatc atacgactgg cggttttcca tatcatccgc caccatgtcg
     6961 tcgtgtttat cccccatcag gccattaatg gcggcgagga agcgggaaat ctgtaacgcc
     7021 tctttatttt caggatcaag cgatcttaag gtgtcgtaat aagaaacctg ctggctgatg
     7081 atttttcgca catcatgaaa ttccctgccg gtgagtcgcg gtttggcaat tatctctttc
     7141 agggtatgca tttgcttttg ccgataggca ataaaaccgc ttacgctttc taactggcta
     7201 tggcgtaatg aatagttcac caacgaccag accggagagc ttaaataaac ccgtaacaag
     7261 ttgccgtaat acgacacaat atttttctta ccgttacgaa aaccgtcctg aaaacgcccc
     7321 aaaaaaacgg tcgttttgcc gaataaaaat ccggcctgat gcttctttaa atacagccgt
     7381 tgcgcaaaac gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt gcgctcatca
     7441 gcagtcagct catcgccttt ggcctgtttg catattaaac gcagtaactc ctggcgattg
     7501 acgccattga gccagaattg caggcataac gcgtaattat catggatgat ttcggtggta
     7561 caacttagtg cgatagtcgg cggcaaactg acctgtgcat caacgacatc atcttcttca
     7621 actgcaacaa ataaacgctc gctcgcctca agcgggaaat attttctcat catcaggatt
     7681 tctctccggc tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt aaggtcggaa
     7741 ttaacgagag caccacatcc agacattgcc caccgaaata agcaaacgct gctcccagca
     7801 cgccaaacag atgacagaac atgaacagca atacgatgta gccgattgag gcgatggttt
     7861 gcgccacgag aaaagcgcgc tgttttcccg ccatcaatag caatgactcc tgtgggaagc
     7921 ccagcatcga aatcacaatt gcgcccagca tcacctgaat caggtcatac gcttcgagat
     7981 acttaacgcc aaacaccagc gaaataagcg gcttgccgac gatcaacacc gcgagcgcca
     8041 ccagaatacc aattccgccc gccagtaaac ctgacttcac gcctaacaac cacggtctgg
     8101 tggtgcgcgg atctaaacgc atcacctctg ggtaaaaact tttgcccagt aaacccgctg
     8161 gcgttccggc ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg gcagcgggtc
     8221 ctaacacgat ccccaccagc actgtgctgc acgagttacg cgccgaccag atggagtggg
     8281 caatgttggt tgaccagaca aaactccacg cgcctttaat gtgtcgggca gactcaaaca
     8341 gattcaattt gaaggcgtta tggatatttc tgcggcgtaa ttcgcgcgcg gcaaaccacc
     8401 agtacatggt gccgccaacc agattcgaca cgtaccaggc aataacaaaa cccgcaaaac
     8461 caaagtcaaa ataccaggct acgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgctt
     8521 gctgtacagc aattagatcg aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg
     8581 aagccattga aggaatgagc gtgcaataca gcgctgccag ccaaaagctt tggtcatcca
     8641 gacctaatga atgggaaaga aaagggagta aggcaatgcc gccgacaatc gccaccgcgc
     8701 cgctgacgat atccagcgag aaggaaaatg agacgacatt gcggaattgc tgcggattat
     8761 tgttggttaa tgctggtgtt ccgtactgaa ccaccagctg ccatatctga aacttaataa
     8821 aatcgctgat cgacttggcg tacgattgta caatcaccag tacgccaaac atggcgggcg
     8881 tcatcccttt accggcacac gagagcgcca acagacccag taaagcgctc acgacattac
     8941 tggagcctaa ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat
     9001 gtttgatgtt aaaacccgcc aattcagcct gaccttatcg ttgatagtaa aaagtatccc
     9061 gccagcctta agttaaactt cggcggtcag aaacgatggc aaccagagaa accgccttct
     9121 gtgcctgttc cagcacttcg ctgtagggcg ctctggaatc aatctcaaga atttttgtgc
     9181 cgttatagcc aatcgtcgac atgacgccga ttttgtcctg cagctcggca tagtcatggt
     9241 caggcttgcg ggagatggca gtatcaatat caatccccaa acgaatgatt aattcaggac
     9301 ggtattgcgc cattttttgg tataaacgcc gttcgcgctg cgccagaaac atgctgattt
     9361 tcccggtcgc acgttcgacg ccgatccccg gtccgtcata ataaaagccc gaaatttcag
     9421 cctgcgggaa gcgatcgctg accaccagaa cgccactttg cgccagtcgc tgaaccttgc
     9481 gtagattcgc cattcttcgc agcgagaagc agtacataat caccgccgcc catagcgctg
     9541 gagatttggt tttcatgctt tgggttttca tgctttgggt tttcgatgat ttggccgcca
     9601 gtcgcctttc gagccagacg ccaaccaacg gcaatcgttt gattttgtcg ccgtcttcgc
     9661 ccgagagcag ccccagatag cgccgctcgg tttgccagtg ttgttgcagc gatttcacga
     9721 ggtcggtggt cagtgtggat ttaccggtgc catcacaccc gaccaccgca atcagccccg
     9781 gaatgtagtt gggctgcggc gcggttgtac tattaacagt ttgtgtttgt agtgcgtcca
     9841 ttcgttaaat catcccctat ccattttggt gatcaggaaa gcgcagcttg aattgcgcgt
     9901 agtgacgctt gcagaatttc atcggctggt tgccgaccat ccagttcaag gatcctggcg
     9961 ccattaaatg tcagttgcgg cgtaacggcg attttttcct gtaacgccgc cagttggtgg
    10021 tcaggtttac gcgcaaacgc ggtttgttca tcaatgccaa gacgaatcaa caatacgggc
    10081 aaataagatg ccatccattg gtacagcttc agctcgcgct gccttaacat ttttatccaa
    10141 ccgttaccgc ccgtggtttt tgccaattgc gggccatcaa agcgaaaacc cggcacttca
    10201 acctgcgggt agcggtcggt gatgagcaga tagccttgct ggcttttaca caacattttg
    10261 cgaaacttat acgcccgcca gcaggaaagc agatagataa ccagcgcagt catattgcca
    10321 ggcggtgtgg agggcttttc gtgcacatgt gccgctttac ttcgcagata acgcccaaaa
    10381 ggtgcgccaa taacagggag ctgtgaaatc cattcgccaa ttcgcccgga cgattgcccg
    10441 agataaatgt gttctgttgg cattgttgct gccagttcat ttaccaggct tgccgtgagg
    10501 gtcgatttac ctgaaccatc acatcctaca atggcaataa cacgcactgg cgtggaatta
    10561 attattgaca tattacgttg attcacgaaa aacccggcag caaataatgt attgaatatt
    10621 acgtggtccg tgctcagaat atccgttcaa ccttattgtg acaatggtta attgtgacat
    10681 tgacctg
//
LOCUS       DACADA010000148        10642 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.150, whole genome shotgun sequence.
ACCESSION   DACADA010000148 DACADA010000000
VERSION     DACADA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.150"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..1349)
                     /locus_tag="ISB17_003816"
     CDS             complement(<1..1349)
                     /locus_tag="ISB17_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP1981699.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFTAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRGIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
     gene            complement(1488..1883)
                     /locus_tag="ISB17_003817"
     CDS             complement(1488..1883)
                     /locus_tag="ISB17_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAP1981700.1"
                     /translation="MKLWPTLGVAFLLITSMRLSWSLGRENARNEAQASTLKSTVDTL
                     NIISAGVQDMQQVLAQLRAENQQRNQDGEVRREQLRNDIAKDECAHALPDARFTDRLR
                     RHAERARASAVSPAYTADADHAGNASPLP"
     gene            complement(1880..2272)
                     /locus_tag="ISB17_003818"
     CDS             complement(1880..2272)
                     /locus_tag="ISB17_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP1981701.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGNQVSWEWPLYEKIAAAFRQASQEL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(2269..2592)
                     /locus_tag="ISB17_003819"
     CDS             complement(2269..2592)
                     /locus_tag="ISB17_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1981702.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            complement(2595..2795)
                     /locus_tag="ISB17_003820"
     CDS             complement(2595..2795)
                     /locus_tag="ISB17_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1981703.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(2795..3289)
                     /locus_tag="ISB17_003821"
     CDS             complement(2795..3289)
                     /locus_tag="ISB17_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAP1981704.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            complement(3391..4191)
                     /locus_tag="ISB17_003822"
     CDS             complement(3391..4191)
                     /locus_tag="ISB17_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP1981705.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     IANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(4237..5289)
                     /locus_tag="ISB17_003823"
     CDS             complement(4237..5289)
                     /locus_tag="ISB17_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAP1981706.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLAVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(5313..6149)
                     /locus_tag="ISB17_003824"
     CDS             complement(5313..6149)
                     /locus_tag="ISB17_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAP1981707.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            6304..8055
                     /locus_tag="ISB17_003825"
     CDS             6304..8055
                     /locus_tag="ISB17_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1981708.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFMVMEF"
     gene            8055..9101
                     /locus_tag="ISB17_003826"
     CDS             8055..9101
                     /locus_tag="ISB17_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1981709.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(9116..9640)
                     /locus_tag="ISB17_003827"
     CDS             complement(9116..9640)
                     /locus_tag="ISB17_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981710.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVRLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLEAAEGFRRMPLSQIPAATPKARGSNDERKLTFSIETV
                     VDDTPGEVAFVHTPVSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            10364..>10642
                     /locus_tag="ISB17_003828"
     CDS             10364..>10642
                     /locus_tag="ISB17_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HAP1981711.1"
                     /translation="MNPITLQIISNAIVLLGVLVAIGTIIYNVRTAKKTQTANFLFES
                     RQDMQYIESLHTLKQVHRSGKSFRSYVFPCDGCIITDEEMAERRKFQYI"
BASE COUNT         2291 a         2831 c         2887 g         2633 t
ORIGIN      
        1 tctgacaact gaattccgcc attcttaatg ctccagccct gcgcagcagc atctccggct
       61 gtcggcaaca gggagattgt gcgtacggat gcatcttcag acggccccga tggcgtgtcg
      121 ccgccgggcg agggtttgat ttccggtgcc ttaccactga tgaaggctaa ggtgcgaccg
      181 gctacgttca gaatagcggt tgccatacga tcgggaataa tgccacgacg cgcccatgag
      241 ctgaaatgcg tctggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
      301 taatatccct cacttgcagc atccggatct tccgttggtg cgttggtgga ggtattgttg
      361 ccactaccat cggtcaggaa cggcacaaag aaaacgttgt cgctctccct gtttttgtac
      421 gccccgtaaa tggtgtcgta ctgcgtgccg taggtgtttt tccagtaata cgtcgtgtca
      481 ccacaaatcc atggcacact tgccgctctg cctccatgac actgcgtgtt aaactcggta
      541 atgtccgcac gaaactgttt cagcatggcc gtaaacagcg gaggttgctg tgagtacgta
      601 gcggcgctca tgtcaaattc gccctgcatc cagcatacgg ccagcagcat gttttttggg
      661 tttttctgca atgcggcctt cgtgcgcaaa atcaggtcct gatataaagg tttacccaca
      721 ccccagcgag ccgaatcctg actggctcct gtggactcgc tgaatgtccc ctccgcgccc
      781 tgggtgaatg ccgaaccacc acgacagcat ggaaccagta aaatcccggc attctgtgga
      841 atgtaaggca acaggcgttt ggcaatatgc aagccctgtc cgacacagcc atactgccct
      901 ttgctcaggt ctgcattcgg gtgattcagt gcgctcatat cctgaacatc atgcaggcag
      961 tggtctgccg gaatgatgtc gttaaatgcg catgctttac caccgggagt cactgtgttg
     1021 cgacgggcca gttgcttaat gcgtgggtgg ggcgcatcgt aagaatccgg cagcggcatt
     1081 ccctcaccat atgacatggc attggattga ccagcaagta ccaccacgta ataccactcc
     1141 ggcacgctgc cgtgctgggg ggaatcaggt gagatgtctg tagtgccatc ggccaccatt
     1201 gcctgcatta gataccacgg ggtgccgggt tcgtaggcaa cccgcgtctc cccctgaaga
     1261 tgccaccctt tcgcaagatg ttcattcacc ttttgagtca gttcagcctg tgtcatggct
     1321 gtaatcagtg caaagttctt actggtcatt gtgttactcc cgtctggtgt gtcagttcgg
     1381 ctattctctt atccagttca gcaatggccc gtttatccgc gttacaggtt tccagtgcat
     1441 ccagaaggcg gtcgccccat attccgaggt ttccccatgt gggtgggtca gggaaggggg
     1501 gaggcgttac cggcatggtc agcgtctgcg gtataagccg gactgacggc gctggccctg
     1561 gcgcgttctg cgtgcctgcg caacctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     1621 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     1681 cgttgctgat tttccgcgcg gagctgagcc agcacctgct gcatatcctg taccccggcg
     1741 ctgatgatat tcagtgtgtc gacggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     1801 gcgttctccc ggcccagcga ccacgacaga cgcatggatg taatcagaag gaaagcgacg
     1861 ccaagcgtgg gccagagctt catgccggat aggctccgtg tggtaactga aaatgcggtc
     1921 cgtctttcag ggtcttccag tcgccgcccc attccaccgg aatattcagt tcctggctgg
     1981 cctgtctgaa tgctgctgcg attttttcgt acagcggcca ttcccatgac acctggttgc
     2041 cgacataagc cacaacatcc acggcatgcc ctgtgagatg gcgactgttc atggtctggc
     2101 tcttgcctgt ggtcacgagc tgcttctgac gataacgact gcgcaatcct tcggtgatac
     2161 caaaatccac ttccgagatt tccagcgccc gtcgggtcac tttcaccaga tcaggattta
     2221 caccctgcaa attcttttcg ctccggctgc tgaatttaaa tgtgttgctc attcgttcct
     2281 ctccttcacc ctgcgatcaa atattgcaat aaccttgtcg cgtgctttct ctgcgcccat
     2341 aaaaccgatt gaggcaccaa aaaacgtcac tgcatcctca gggatcccga agaagcgcag
     2401 cgacccggcc acggccatgg caagaacgcc gcacgccagc gatcccgtta cggtctgaac
     2461 cagtgttcgt ccgtcataaa gactcatcag cgcggaaatg ctgaccgccg cgcctactgc
     2521 atacaccgtt ggcaggtggt caaagagcca cgcaataacc tgctctgtga tccctgtttg
     2581 aatggtgctc actgctactc cccccacaac tgaatcattt ctcgtttctt cttctccggc
     2641 tccggcatct ccacttcctg cccggcgtcc agaaatacct gctgacagag tccggggttg
     2701 gcatccagca ccttttcggt gacgccctgt gtcgtgccgt agtaccggaa acagagcgaa
     2761 tccacggtgt cgccttccag tgccttcact ttcatcagca cagctccgca aacattcgcg
     2821 gtcgtcccag aatgtcagag atggcccagc tcacatcgcg ccacaaatcc gatgtctgta
     2881 tatccagtgc gtccgcccgg cggtcgccct tgtccgttgt gtccgcatcg cggtaacgct
     2941 ccagaatcag ggcgcgtgtg gcggtataaa cagcattgcg ccagtgccag agattgacgc
     3001 tttctccgtt aattacaggg gccggaacat cggccagcgt ctgatagcca gccgcctgtt
     3061 gttcctgctg ccatgcttcc agctcgcggg taacgtgtgc tatggccccg gtggcggtat
     3121 gcagcaggcg ggaggtggtc acgcggcccg gcagtcgtac cgccagacgc agctcgcgca
     3181 gcacaatatc cggccagaat gcacccgctg aaatacgggt atcgccatca tcggtgtcgg
     3241 tgatgtcgtc ctctgcgggg ccggggttag ttctggcaac catactcatg gggttcactc
     3301 ctgaaaaaat cgggcggtgg gtgcgcggtg taaacggtca cggagtcaaa ccggaacacc
     3361 gcgcacgccg cccgctgacg gggtcagtcg ttaaccgcgc tttgccttct gcgtcgcggt
     3421 ggtttttcgt gttgcaggct tccgcgttgt ctttttactt ttgctgcttt cgtcctgcgc
     3481 ctgctgtgcg ctggcgtctt ctggttcggc tgcggaatcg gcttttttca gggcgcggga
     3541 aagggttgca atctcgcgtt tcacacctgc gttcgggttc aggtgcatcg cttcgcgcag
     3601 cagcttcagt gatgaggcca tgttgtctgc atcggtcagg ccacgacggg caaaggcaca
     3661 cgccttgcat aatttggcgc gcacttcgtc cggcatgtcc tggttggcga taatttccca
     3721 aagtgtgtcc agtggttcga taaaggcgga taaatccgcg tcggcatccg tcccagcctg
     3781 cgtcagtacc gggttgcaga tttcttcggt cagtaccgtg gcagcagtac ggccaaagtt
     3841 atccggcatg atgaggttgt gacggaccac atatgcacca atacgcagcg ccagcggaag
     3901 atcgccgcag tcaatcgccc acaccatcag cgtggcaatt acttcgtcct gttgcccgcc
     3961 gtcagcctcc agcgttccct caatccagcc ggaaaagtcc ggcaacaact cttttttgat
     4021 ggcggctttc gcgcttctgg cctgtatggc ctttagcctg gcctgtgcca gacgcagacg
     4081 atacagcacc tcttcatgcg cggtacacgc ggcgtggtcc acaccttcat tcgcccggcc
     4141 tgcgcgctgt gccatcacgt tctgccagtg ttgttgtgca ggagtaatca ttttttctct
     4201 ccgttacagg cgggcatgat gcccgccgtg agttgattag ctgtcggcga acttcaggcc
     4261 agtgaccatc gcgcacttgc catagtcttc aacgacataa gcgtcattga tggactggta
     4321 ggtggcgatg cggttgtatt ccggctcgtc tttcatcagg cgacgcattg aacctttctg
     4381 ccagtaaatc gacaggttgt tgaacgaggt gataagcatc gttgcatccg ggaagaacgg
     4441 cgcaaggaac acgcccagcc cgccaatggt gcgcgatgac aggatgagct gcccggcaag
     4501 taattccgca ttgggattct ggccgctgat gctgttcagc acgggcagac gcagcgagtt
     4561 aaacaggttg cgccccataa tcaccgcgag gtcgtcagct tccttgtgcc attcatccag
     4621 cagggatgag cgcgcatcct gtaccagagc atcggcattc gcatacttac ccgcgtgcgc
     4681 cacagtgttg tccatgttgc gggaggtcag cgtcacgtca ttcattacgc gctcgctggc
     4741 atcggttctg atgtgctcca gccagcccac gttaacgtcc tgaagcagct tgttggtgtg
     4801 aaaatcagac tcgtaggcgt gggatgtgcc gttaaagcca atcatgatgc gatcaagcgc
     4861 tacctgccgg gcgatctgtg tactgatacg ggactgaaaa tcgctgtggg ccgcccaggt
     4921 atcaagctgt gggtacgaaa taaaggtatc gtagttcacc tgttcgcact ggtactgacg
     4981 agacttcata tcgatcacgt tattcgggtt acggcgtttg atgccgtcat aactgctatt
     5041 cgtgcgcgca attggcccgg tggtgtccag gaggattttt tcgcctttct ggtcggtcac
     5101 gccgatcacg ttaattcttt tcgtaaattc ggtactttcc tttgaggcgt tttcaaaacg
     5161 ctgctgcacc gcgggttcca cggtaaatcg cgataccagt gcagataccg ggatattgtt
     5221 aagcgacgcc tgctgcgcca tatagcaacc cagcttgttg cgggtaatat ctgacatcac
     5281 cagattcata aaaaatttgc tcctttgtct tatcagaagt cagccagctg gtcggaggct
     5341 gcgcccgttg cggtgaagcg gttctgcgga tcgccgtcct gcgtgcgcag tttttccttc
     5401 aatgctgtca gctctgtggt cagtgacgtg attttctggc ggtcctgctg atggcgggtt
     5461 tccagcacat taaaacggtc gataatgtcg gcctgtgacg ttgcaacgcc ttccaccgct
     5521 tcctgaattc gggagaaact ggcgtcatcc gctttgcggc cacgaccaat aatccccatt
     5581 acgcggttaa accactgggt gccttcttcc tggcgttgtt ctgccatttc gatgatttca
     5641 gactcgatgg cttcggagat aagcggtgct tcaccctgga tactgttgaa cgtcatcacc
     5701 gcctgacgtt gctgtgccgt gaatttcagg cgctcagtgc ccaggcttgc cggggtgtcg
     5761 gtcatcgcca gcccgaccag ataggcgcgc ccgttaacgg agaactgcgg gtgcagttcg
     5821 atactggaat agattttctt gccgtcagcg acaagctgct tcatgcgctc ggtcggttcg
     5881 atttctgcat acagcgcagt acgtccggcc agcggacctt ccgtaatgtc ttccgtactc
     5941 agtgcggtga catcgcccat tgcggaaaat tcgcttgacg gacatggcga gagatagtgc
     6001 tcaacgttca cgcgggcagc gtaaacatcc gggttgaagt tctcggcggc ttcacgcaga
     6061 tgcaccggac tgatttcacg gccatcaaca gttgatccgg agacagccac gcgaaacttt
     6121 ttgcgggatg tctttttttc attagccata gtttttgccc ctctgactgg ttcttcagtc
     6181 atgatggcaa agcgtaacag gctgatacaa agggcttttg ttgtaagaaa acagtcagaa
     6241 cagagggtta aggagaacag tttcgcgcgc gggtaatctt cctgtaatta ctcaggggga
     6301 gcaatgattc aggacgcttt tgtgcgccag cgtgcgcggc aactttactg gcagggttat
     6361 ccgcccgcag aaatatcacg tctgatggga ataaacccga acacgattta tgcgtggaaa
     6421 aaacgtgacc agtgggatga aacgccaccc gtgcagcgtg tcacgcagtc catcgatgcg
     6481 cgcctcatcc agcttactga aaaacagaat aaaacaggcg gtgactttaa ggaaatagac
     6541 ctgctgaccc ggcagcttaa aaagctgcat gatggccagc cggatgtgat ggccgcagga
     6601 aagaaaggcc gggcgaaaaa actcaaaaat catttcacgc cggaacagat tgccgcactg
     6661 cgggaaaaaa tcatcagcag gctggagtgg catcagcggg gctggtttga ctccctgacc
     6721 ctttgcaggg aagccgggat acgtaacagg atgatcctga aatcccgaca gattggggcg
     6781 acctggtatt ttgcacagga agcactgctg atggcgctgc gtgacgatgt ggcgcaacct
     6841 taccagcgta accagatttt tttgtctgcg tcgcgtcgtc aggcgttcca gtttaaaagc
     6901 attattcaga aggccgcggc tgaagttgat gtggagctga aagggggcga taaaatcatc
     6961 ctctccaacg gcgcagagct gcattttctc ggcacttctg ctgcgtcggc acagtcctat
     7021 acgggcaatt tttattttga tgaatttttc tgggtcagtc gctttgctga actgcgcaag
     7081 gtggctggcg ctatggcaac cctcagcgga ctgcggcgca cctacttctc cacgccatcc
     7141 accgaaacgc acgaggcata cgcctactgg aacggcgacc gctggaacga gaaaaaggcc
     7201 tcgcataaac gccagcgttt ttctgtggac tggaaaacgc tgcataatgg gcttatctgc
     7261 cctgaccgga cgtggcggca aattgtcacg ctggaagatg tggttaatca cggctggaaa
     7321 cacaccgata tcgacgaaat tcgtgatgaa aacaccgaag acgagttcct caatctctat
     7381 atgtgtgagt ttgtccgcga aggggaatcg gcatttaacc tgaatatcct gattggctgc
     7441 ggtgttgacg gatacgacga ctggaaagac tggaaacctt ttgctccccg cccgatgggg
     7501 aatcgtccgg tatggattgg gtatgacgca aacggcagta gtggcaacgg cgacagcggc
     7561 gctgtgtccg tggtggttcc tccggctgtt cctggcggcc gttttcgaac ggtggagacg
     7621 cgacgcgttc aggggctgga gtttgaagaa caggccagag tcattgaaga gttcacgtgt
     7681 cgctacaacg tggaacacat cggcattgat gtgacgggcg ggaacgggga ggctgtttat
     7741 cagatagtga agcggttttt ccctgctgct attccgtaca ccttcacgct gtcatcaaaa
     7801 cggtcgctgg tactgaaaat gctgcaaata atgcgtgccg ggcggtggga atacgatcgc
     7861 gccgaacgcg agctggtcgc ggcctttaac gccgtgcgta aggtgaaaac accgggcggc
     7921 tttatcactt acgaaacgga ccgcgcgagg gggatcagcc acggcgacct tgcgtgggca
     7981 accatgcttg ctgtcattaa cgaaccaatt ggcggcgaag gagaaaacga gcgtttcatg
     8041 gttatggagt tctgatgagc agaaaaaata aaaaagtgcg catgagttca cgcattgatc
     8101 tcgctgatgc gctcaggaaa gaatcatcgc tcagtgcatt cacatttgat ggtccttatc
     8161 gcctgaccgg gcatgacctg ctggacaata tgtactgtgc tgataacggg cggtggtatg
     8221 aaaccccggt ggactggtac ggtctggcaa gagcagcccg gcaaacgtcc tggcatcagt
     8281 ctgcgcttta ctttaagcgc aatgtattac tcggttgcta catcccgcac ccgctgcttt
     8341 cccggcagga tttctcggcg ctggcgctgg actggtttgt gttcggtaac gcattccttg
     8401 agcttcgaag caatatgctc ggcgaaccgc ttaaattacg gcacgccctg gcgaaataca
     8461 tgcgacgcgg aagcgatctt gaatcatggt ggtatgtgca ggatggcaag gacgcgtttc
     8521 agtttcgtcc tggcaaagtg tgccacctga tgaatccgga tattaaccag gaaatctacg
     8581 gcatgccgga atatcttggc gcattactct cggccagcct ttctcattcg gcggacatgt
     8641 tcagaaaact gtactacgac aacggatccc acgccgggtg catcatctac atcggtgcag
     8701 cgcaggtaaa ccgcgaaagc atggactccc tgaaagaaac gctacagggg gcacgtggtg
     8761 gtggtgcgtt taaaaacgtg ctcattcatg cgcccaacgg gggcaaagag ggggtgcaaa
     8821 ttttgccgtt ccagcagatc accgcaaaag atgaattcat gaatgttaag gcggcatccc
     8881 gtgatgatgt gctggctgcg caccgcgttc cgccgcaact gatgggggcg atgccgggcg
     8941 aaaaaagtgc gtttggcgat gtggagaagg cagcgcgggt ttacgcaatt aacgagctga
     9001 tgcccgttat ggaggctatg aagcacatca atgactggct tggcgaagag gtgatccgtt
     9061 ttaactctta tgctcttctt gatgaaaaaa cagccccgtg atggggctgt cctttttacc
     9121 agagttgaac cattttctgg gtgccgtcag gcttgagatt atcaatttca gagagaacgt
     9181 aatattgaat ggcttcacaa acggtggtgt agggtgaatt accttcttta agtggcacga
     9241 tattattatt aacgcgaacc tgtatttcat cgttatacat tgcgatcgaa acaggagtgt
     9301 gcacgaacgc gacttcgcca ggtgtgtcgt caaccaccgt ttcaatacta aaagtcagtt
     9361 ttcgctcatc attgctgccc cgtgctttgg gggtagcggc agggatctgg gataaaggca
     9421 ttctgcgaaa tccttcggct gcttccagtc cgcatgaaac gtaatgctgg cgattaccgt
     9481 cgctatctgt ccaggtctgt gatggcagct ccagcgagat ttcataagca tcaacaatgc
     9541 cctgggcaag gcgtacaagc ggggtcagat cttcattgcg gcgaaagctc tcctttacct
     9601 gctcccgttt ttctcttaac tgcttgtaat taatgaccat aagacagcct ccattgattt
     9661 ctttgctcgt attttgcact tatgaagtat ggtcggcaag gtgccgtatc gctgacgcgc
     9721 ttcgcttgtc tgctgcttcg ccggggcata aaaaatttat gccccggctc tccagctcct
     9781 gtatcaatca gataatttca cgacgccttc cagtttatcg ccaccatcga cggtcagact
     9841 cttacgcaat tccaccgcgt tgactgcatg ttctcgctgc ctcagtgcga ttttgacggc
     9901 cttacctttc accccatcaa atcaaaagcc ctcacgcctt tttcatgctc agcgtgagaa
     9961 atatggccat tctgttgtgt ctctgcgaca tcgttcaggg aatgctattt accccctgaa
    10021 acgcgggctg ttcccccgtc acctgcgcgc agaaaaagcg cgtttttttg tgcacgcacg
    10081 gatccttgac ggatccagcc gccatgcggg ccggaagggt aaaaagtcgt tcaaaaaaat
    10141 tgtgcaaatt tgtgcacttt tgtgcaggta agcatgccta tatatttgac cgtggcttgc
    10201 ctttgatgta gtttatacgt cacaaaaacc acaaaacgtg gttatgattt ctatgagggg
    10261 gttgatatga aaaaaatgta tgatgaattt gatggtttct aagctacgtt gacctctaat
    10321 gaggcgggga tttgctcccc gcctttttta tggatgaaat aggatgaacc caataacact
    10381 acaaatcatc agcaatgcta ttgttctgct tggcgttttg gttgccattg gaaccattat
    10441 ctacaatgtg cgtaccgcaa aaaaaactca aacggccaac tttctttttg agagtcggca
    10501 agatatgcaa tatatagagt cattgcatac cctgaaacaa gtgcatcgtt caggcaaatc
    10561 atttcgttct tatgtttttc cttgtgatgg ttgcataata acagatgaag aaatggccga
    10621 gcgtcgaaaa tttcaatata tt
//
LOCUS       DACADA010000149        10620 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.151, whole genome shotgun sequence.
ACCESSION   DACADA010000149 DACADA010000000
VERSION     DACADA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.151"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            16..663
                     /locus_tag="ISB17_003829"
     CDS             16..663
                     /locus_tag="ISB17_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1981712.1"
                     /translation="MVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQE
                     LSLKSGLVCHLGAMESISAIYLDKIESPDSVPTSKSWIGKKIELHITALGKALLAWKT
                     REELDYFLEALTLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVF
                     NMYNRVNYAISLSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            668..1573
                     /locus_tag="ISB17_003830"
     CDS             668..1573
                     /locus_tag="ISB17_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1981713.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            1584..3551
                     /locus_tag="ISB17_003831"
     CDS             1584..3551
                     /locus_tag="ISB17_003831"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HAP1981714.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQIKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            3658..5007
                     /locus_tag="ISB17_003832"
     CDS             3658..5007
                     /locus_tag="ISB17_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HAP1981715.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            5354..6340
                     /locus_tag="ISB17_003833"
     CDS             5354..6340
                     /locus_tag="ISB17_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1981716.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(6381..7385)
                     /locus_tag="ISB17_003834"
     CDS             complement(6381..7385)
                     /locus_tag="ISB17_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HAP1981717.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVACVVSAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(7489..8130)
                     /locus_tag="ISB17_003835"
     CDS             complement(7489..8130)
                     /locus_tag="ISB17_003835"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP1981718.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGIPEAGKIPSSEWLFHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(8161..9276)
                     /gene="mtnA"
                     /locus_tag="ISB17_003836"
     CDS             complement(8161..9276)
                     /gene="mtnA"
                     /locus_tag="ISB17_003836"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HAP1981719.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQEMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(9286..10545)
                     /locus_tag="ISB17_003837"
     CDS             complement(9286..10545)
                     /locus_tag="ISB17_003837"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HAP1981720.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWPTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
BASE COUNT         2684 a         2648 c         2634 g         2654 t
ORIGIN      
        1 aatttattga atgtaatggt ggagtgtggt ttcttaatta aaaacaaaaa cggctactat
       61 tcactgggaa taaaaaacta cgaattgggt tgtcaggcgt tacaccgtca aaatatattt
      121 gaggtgacta agcggccaat gcaggagttg tcgctgaaaa gtggactggt ttgtcatctt
      181 ggggcgatgg agagcatttc ggctatctat ctcgacaaga tcgaaagtcc ggactctgta
      241 ccgacgagca aaagctggat tggcaaaaaa attgagttgc atattaccgc gttgggtaag
      301 gcattattag cctggaagac acgagaagaa ctggattatt ttttagaagc actcacgtta
      361 actccacata cgcgcaatac atttaccgat aaaaaattgt ttctggaaga attacaaaaa
      421 acacgccttc ggggatgggc catagacaac gaagaatcaa cctatggggc ggtatgttta
      481 agtatgccgg tattcaatat gtataacaga gttaactatg caatctcact ttctggcgat
      541 ccggtagttt attcaggaaa taagatagac agctatctgg aattgctccg gaaatgcgct
      601 gagcaaatat catatgggct gggatacaga aacgaaaatg agcatttacg aaaaggaaac
      661 tgaggtaatg aaaaaattca gcggcattat tccaccggta tccagcacgt ttcatcgtga
      721 cggaaccctt gataaaaagg caatgcgcga agttgccgac ttcctgatta ataaaggggt
      781 cgacgggctg ttttatctgg gtaccggtgg tgaatttagc caaatgaata cagcccagcg
      841 catggcgctc gccgaagaag ctgtaaccat tgtcgacggg cgagtgccgg tattgattgg
      901 cgtcggttcc ccttccactg acgaagcggt caaactggcg cagcatgcgc aagcctacgg
      961 cgctgatggt atcgtcgcca tcaaccccta ctactggaaa gtcgcaccac gaaatcttga
     1021 cgactattac cagcagatcg cccgtagcgt caccctaccg gtgatcctgt acaactttcc
     1081 ggatctgacg ggtcaggact taaccccgga aaccgtgacg cgtctggctc tgcaaaacga
     1141 gaatatcgtt ggcatcaaag acaccatcga cagcgttggt cacttgcgta cgatgatcaa
     1201 cacagttaag tcggtacgcc cgtcgttttc ggtattctgc ggttacgatg atcatttgct
     1261 gaatacgatg ctgctgggcg gcgacggtgc gataaccgcc agcgctaact ttgctccgga
     1321 actctccgtc ggcatctacc gcgcctggcg tgaaggcgat ctggcgaccg ctgcgacgct
     1381 gaataaaaaa ctactacaac tgcccgctat ttacgccctc gaaacaccgt ttgtctcact
     1441 gatcaaatac agcatgcagt gtgtcgggct gcctgtagag acatattgct taccaccgat
     1501 tcttgaagca tcagaagaag caaaagataa agtccacgtg ctgcttaccg cgcagggcat
     1561 tttaccagtc tgaggagaaa ctcatgtctg ttcgcaatat ttttgctgac gagagccacg
     1621 atatttacac cgtcagaacg cacgccgatg gcccggacgg cgaactccca ttaaccgcag
     1681 agatgcttat caaccgcccg agcggggatc tgttcggtat gaccatgaat gccggaatgg
     1741 gttggtctcc ggacgagctg gatcgggacg gtattttact gctcagtaca ctcggtggct
     1801 tacgcggcgc agacggtaaa cccgtggcgc tggcgctgca ccaggggcat tacgaactgg
     1861 acatccagat aaaagcggcg gccgaggtta ttaaagccaa ccatgccctg ccctatgccg
     1921 tgtacgtctc cgatccttgt gacgggcgta ctcagggtac aacggggatg tttgattcgc
     1981 taccataccg aaatgacgca tcgatggtaa tgcgccgcct tattcgctct ctgcccgacg
     2041 cgaaagcagt tattggtgtg gcgagttgcg ataaggggct tccggccacc atgatggcac
     2101 tcgccgcgca gcacaacatc gcaaccgtgc tggtccccgg cggcgcgacg ctgcccgcaa
     2161 aggatggaga agacaacggc aaggtgcaaa ccattggcgc acgcttcgcc aatggcgaat
     2221 tatctctaca ggacgcacgc cgtgcgggct gtaaagcctg tgcctcttcc ggcggcggct
     2281 gtcaattttt gggcactgcc gggacatctc aggtggtggc cgaaggattg gggctggcaa
     2341 tcccacattc agcccttgcc ccttccggtg agcctgtgtg gcgggagatc gccagagctt
     2401 ccgcgcgagc tgcgctgaac ctgagtcaaa aaggcatcac cacccgggaa attctcaccg
     2461 ataaagcgat agagaatgcg atgacggtcc atgccgcgtt cggtggttca acaaacctgc
     2521 tgttacacat cccggcaatt gctcaccagg caggttgcca tatcccgacc gttgatgact
     2581 ggatccgcat caacaagcgc gtgccccgac tggtgagcgt actgcctaat ggcccggttt
     2641 atcatccaac ggtcaatgcc tttatggcag gtggtgtgcc ggaagtcatg ttgcatctgc
     2701 gcagcctcgg attattgcat gaagacgtta tgacggttac cggcagcacg ctgaaagaaa
     2761 acctcgactg gtgggagcac tccgaacggc gtcagcggtt caagcaactc ctgctcgatc
     2821 aggaacaaat caacgctgac gaagtgatca tgtctccgca gcaagcaaaa gcgcgcggat
     2881 taacctcaac tatcaccttc ccggtgggta atattgcgcc agaaggttcg gtgatcaaat
     2941 ccaccgccat tgacccctcg atgattgatg agcaaggtat ctattaccat aaaggtgtgg
     3001 cgaaggttta tctgtccgag aaaagtgcga tttacgatat caaacatgac aagatcaagg
     3061 cgggcgatat tctggtcatt attggcgttg gaccttcagg tacagggatg gaagaaacct
     3121 accaggttac cagtgccctg aagcatctgt catacggtaa gcatgtttcg ttaatcaccg
     3181 atgcacgttt ctcgggcgtt tctactggcg cgtgcatcgg ccatgtgggg ccagaagcgc
     3241 tggccggagg ccccatcggt aaattacgca ccggggatgt aattgaaatt aaaattgatt
     3301 gtcgcgagct tcacggcgaa gtcaatttcc tcggaacccg tagcgatgaa caattacctt
     3361 cacaggagga ggcaactgca atattaaatg ccagacccag ccatcaggat ttacttcccg
     3421 atcctgaatt gccagatgat acccagctat gggcaatgct tcaggccgtg agtggcggga
     3481 catggaccgg ttgtatttat gatgtaaaca aaattggcgc ggctttgcgc gattttatga
     3541 ataaaaactg aaagcataaa taaaaacatc cagacacctg aattatccga ctctgcatca
     3601 ttcgttattt gctcagtcgg ataatgatgg gtcgctaccc tgttatgaga ggacgttatg
     3661 ccactaatta tcgttgtggc agggattgct ttactcctgc ttttaaccat aaaaattaag
     3721 cttaatacgt ttgtttcgtt aattattgtc tcgattgctg tcgccatcgc cagtggtatg
     3781 gatttgagta aagtcgtcac ctcagtcgaa tccgggcttg gcggtacgct gggccatatt
     3841 ggtttgatat ttggcttcgg tgttatgctc ggtcgcttgc tggccgatgc gggtggtgcg
     3901 cagcgaatag cgctgaccat gctgaattat ttcgggaaaa ataaacttga ctgggcagtg
     3961 gtctgttcgg cgtttattgt cggtatcgca ctttttttcg aagtcggttt aattcttctg
     4021 gtacctattt tattcgctat tgcccgtgaa gcaaaaatat caccgatgtt tatgtgtgtg
     4081 ccgatgcttt ctggtttgct ggtcgcacac ggttttttgc cgccgcaccc cggcccgact
     4141 gttatcgcca gggaatatgg cgctgatgta ggattggtac tgatatacgg tatcattgtc
     4201 ggcattccaa cctttattct ttgcggcccg gtattgaata aattctgcca gcgcattatt
     4261 ccggacgcct ttaaaaaaga ggggaatata gcctctctcg gcgcgacccg ccgattcagc
     4321 gaaagcgaaa tgccaggctt tgggatcagt tttctcacgg caatgctgcc ggtgatcctg
     4381 atggcggttg tcaccatcat ccagatgact catgccaaaa gtgccgcgga ttctggctta
     4441 ttttacaacg tgatactgtt cttaggtaat tcgacgatcg ccatgctgat ttcactgctg
     4501 ttcgcgatct ataccatggg cctggggcgc gggaaaacaa tccctgacct gatggattct
     4561 tgcggaaaag ctatcgcggg tattgccggt ctgctgctga ttatcggtgg tggtggtgca
     4621 ttcaagcaag ttctcatcga ctctggcgtt ggtcagtata tttccaccct ggtttcgggt
     4681 atggatatta acccgatctt gatggcctgg ggcgtggccg cattcctgcg tatttgtctg
     4741 ggttcagcca ccgtcgcggc gatctctacc gccggtctgg tcatcccatt gttggcagta
     4801 catcccaata ccaacctggc cttgattacg ctggctaccg gtgccggatc ctgtatttgt
     4861 tctcacgtta acgatgccag tttctggatg attaaagact tttttggcct gaccaccaaa
     4921 gaaacgctgt tatcctggac gttgatgtcc acactcttat ccatcagtgg gctgattttt
     4981 attctgctcg ccagcttagt tttataatcg ggttcttgcc agtgagccaa cctgttgttc
     5041 actgtccccc ataatttagt gagtgacggc aaccgtctgc acggtgacga atcgctggcg
     5101 atcaaaaagg ggcgatattg aaccgtcgat tatgcttgat gggtgtactc tcagtaatac
     5161 tgtaaagccg gagcatgtct ccgactttac agtatggggg aacagcaggc gatcgctcaa
     5221 atggtatgct ggtttatttc atgtaaaaga ataaaatatc tgtataaaat catgccactg
     5281 gcaatctgca tcaactctcc gggtaaacag ggttaagatg agcatttccc cggtttaata
     5341 caggtataca aaagtggata gagatcaaac aaacagcagc ctgttcaatg atgatcccgt
     5401 acttcatgca acatggctgt actatcagga aggcaaaagc cagactgaag ttgcagctat
     5461 catgggggtg tcccgcgtca cggttgtcaa atacctgcaa acagcccggg aaaatggact
     5521 ggttcatatc aacctggatg tgaatgtctt tggctctatc gacgctgctt tgcaaattcg
     5581 cgataaattc aatctccaga gagtgatcat cgtgccggat ggcgaacatg ccggaaaacg
     5641 tgatgataca aagctgatgc ggactcgcct ttcacgtgcc ggtggcatgt atctgaatca
     5701 ggtgattgag aatggtgatg tgctgggggt tgcctgggga agaacaatcc atcagatgag
     5761 caaaaccatg acgccgaagt catgtaaaaa tgtcaccgtt atacagatgc tcggttccat
     5821 gccatctcag ccagacctga cgatcattga atcctcatcg caaatcgcct acaagctttc
     5881 aggccgtgtc gcctcgctgc acgtgcccgc cgtggtatca agcgccagac tggccatgga
     5941 gttacaggca gagcctatta ttcgatcgaa ttttgacgtt ctgactcgct gtacgaaagc
     6001 cttttttgtc gtgggtaacg cgcttgatga aaatcccctg attcgggtgg gcgtgctgaa
     6061 taagaaggaa atgcagacgt atcgggattt aggcgcagta ggtgtcattt gtggtcgctt
     6121 ctatgacaag gaagggatgc ccgtcgtggc tgatgttgac cagcgtattc tgggaatcag
     6181 tctggctcag ctaaggcaga ttgaacggaa gatcttcctt gctggtgggg aaagaggcta
     6241 tgacgcaacg ttgggtgccc tgctgggagg atatgtcacg gacctgattg ttgatgaagg
     6301 aacggcagag tttttgctgg cgtgtgaatt accacactaa aagaatgaac aactgcccca
     6361 tgtggatatg gggcaggatt ttattgatta gcggcgataa taagaacaat acccacaatg
     6421 tatgaaataa acatggtcag cagcagttta ttggttccgg ctggcgcact ggcaaattct
     6481 ctccagatga agactcccca gatcacggca atcattgtgg ccccctgtcc cagaccgtag
     6541 gaaatggcat accccgcctg accagacgca atcagactga atgccagacc gacacaccaa
     6601 atcataccgc ccagccagcc gcaaacatga tcccgtaagc tcccggaaaa atacattttc
     6661 ccgttgaccg gttctcctga aataggtttt ttcatgacca gcgtgttaag gacaaagttc
     6721 gacaagaaca gccccacggc aaacagaaca agtgctgagt aaggcgtcat cagaccgctg
     6781 gcaggctggc tgaaattgtc agacatggag tccgccaaga agcggaaaaa ccatcccatg
     6841 atgatacccg ccagaatggc agtaatgaga cctttattac ggcgagattt atccgcctcc
     6901 tgcgtgacac gaccataggc tatcgcggta aaaataatgg cgctcacaac gcaggcgaca
     6961 ccgaggaaca gaatgagcgg atcgccctgc ggattgccga tataggtggt gatcacaccc
     7021 agagccagtg ccagccccac acccacagga aaagctaccg ccataccggc aagattaata
     7081 gaagcgacca gaagaatatt ggaaatattg aagataatcc ctgccagtat tgccgacatc
     7141 agactactgc tgctcgcctg ctgaatatca ggtataaaac tgcgaccggc ttcgcccata
     7201 gaacccagcg tgaaggcaaa taacaaagaa cacagcaata atccaattga ataatcccaa
     7261 tagaatagag ggaactccca ttttttattg ctgacaagtt tagttgtatt cgcccatgaa
     7321 ccccagcaaa tcatggtaat aaaacacatt attatggcga cagcatacga ttccacgata
     7381 aacataaata actccttgtg gtagttataa tcaatcgttg tggacagagg gaatagcagc
     7441 cattctgcga atggctgtca tatgttatta attaaaataa agctgtaatt acgctttcaa
     7501 accatacgtt ttgaattttt caaggacgat ggtcatttcg gcttcagaca gcacgggaac
     7561 atcgtgatgt aatccgccac atttgatgta taaatcagcc agcgtctcgg tttcacccgc
     7621 cagccacaac gtcttttcca gcgtgacgtc catggccagc ataccgtggt gctgcatcag
     7681 caaggactta ctttctctga ttcctttaga tacaccgtcg gccagttcag gactgccaaa
     7741 ggtataatag ggaatacagg gaatatgatc cgtccccgat accgccacca tataatgaat
     7801 ggccggaatt gaatgattat gtattgccac cgccgtggaa ttcaccgcat gcgtatgtat
     7861 gacagcattc aattctggtc tggctttata acacgccaga tgaaataacc attcacttga
     7921 tggtatttta cccgcttcag gtattccctt atcgtctaca aagacaatca tgtccggcgt
     7981 catctttgaa taagcgatcc cactcggcgt aatcaacatg ccaccggtat acctggcact
     8041 cacattacct gatgttcctt gattaagacc tgacgcattc atttcccgac aagtggaaat
     8101 gattttttct gctaacttaa tccgttccat tcctgatcct taatagcaat ggccgccgtt
     8161 ttaatgctgt tccccctgga gtgctttact cttcaggtca gcaaacatat ccgccagcgc
     8221 tgacttactg gcagcacaga ctccccgctc agtaatgaag ccggtaatgt atcgggcagg
     8281 cgtgacgtcg aaagcgtagt tcccacaacg ggttccttca ggggcggtat tgacccagct
     8341 caattctccc tgagggttta tcccatagac atgggactgc tcttttccgt cacgttgctc
     8401 aatcggaatg ctttttccgt cctcgatagt ccagtcaata gtgggagaag gtaacgcgac
     8461 gtagaaggga acatggttat catgggccgc cagcgcctta agataggtac caattttatt
     8521 acagacatcc cctctggccg tggttcgatc ggtcccaaca atgcagagat caacgtcacc
     8581 gtgttgcatc aggtggccac ccgcgttgtc ggcgatcagg gtgtgaggaa taccgtgtga
     8641 gcccagttca aatgccgtga gtccgccctg attacgtggc cgcgtttcat ccacccagac
     8701 gtgaacaggg attccgtttt catgcgcttt gtagatggga gaaagcgcgg ttccccagtc
     8761 tacagtcgcc agccagcctg cgttacaatg tgtcaggata ttgaccacac tcccggccgg
     8821 ttttttctgg gctatttcgc ggatgacttc tgtaccatgt aatccaattt gttcacacaa
     8881 cgcgacatct tcctcggcaa tttctgccgc cagcgcccag gccacatcct tgcgctctga
     8941 tacacagaga tccttcagtg tgtctatcat cctgtcgagg gcccatttga gattaattgc
     9001 cgttggtcgg gttttgataa gaaggtcata gtaacgctgc agacccatgt cactggcatc
     9061 gtgattcatt cccagcgcga tgccataggc tgcaacgaca ccaataagcg gagcgccacg
     9121 aacccacatc tcctgaatag ccgtagcggc catttccgca gaggtaagcg ccaccacctc
     9181 gaacttaaaa ggcaacttag tctgatcgat gatttctacc gcttttccat ccccggaaac
     9241 ccagaccgta cgatagtgtt tacctttaat attcataatg acctcttact taaccttcgc
     9301 agactggatg acggaatcca tatttttaaa ctggcgggca ttgacgataa gatcgcgcgc
     9361 catggtcaat gcacgtcgtt cacatcctgc gcgaagctct gtattttcaa tctgtttcag
     9421 ctcggcaacg ccagcaaaac caatgatccg gcgattcatt tccatgccgg cattcaccag
     9481 ggaatcttca agcagcgtgg caaaaaactg atcctgtgcg tcctcaagag cggccctgtt
     9541 ctgttgatac atttctgtcg gccacgcgtc gccctgtgtt ttgttgtccc agagctggcg
     9601 gaactcccgg gtgaaaacgg accaggtttg gacaatctgc tgaagcagcc attcctgata
     9661 gtcagcgcga cgttgctcat tcgcatccca cccagggcgt gagaagtacg ccagcaggag
     9721 gttgccaata tagttgccga tatcaaacgc cattggcccc atgaaaccga actccggatc
     9781 aatcactttg gtatccgtgt cggtcaccat gattgagcct gaatgaaggt cgccatgtaa
     9841 caatgcctgc gcttctgtca taaatttgta cttataacgc atggcaacct ggatcatctc
     9901 tacatcagcc caggccttat ggacggcatc gtccagctca ggagaagtcc agttattccg
     9961 ttcagcgttg taatagggct ctgtgaagat cagatcttcc gtaattttgc acagctcatg
    10021 attcaacgcg aactcggcag taagcgcttt tttctgttct gctgacatgc caatgtcaga
    10081 cgtattgaag agagtctgtg ctaaaaatct gccgatatct tcagctagtt tagggaattt
    10141 tttaccgtta atcagttcct tacgcagaat cacgtgagga gtcagatact ccatggcaaa
    10201 cagcgccatt tccgggtcgt aaaaatagac ctgcggaatc agtgtgtgac ccgcgtactt
    10261 ggcttcctga cataagacgt tgtactcaaa gcccgcacgg ctcagagata aaggccatcc
    10321 ttcgccccct gcacgaagcc agggcagagc ttgttttaca atgatggttt tatgtgtccc
    10381 ctcaacgata aacaccatgt tcaggtttcc gtcccccact tctgaaactt tccactcttc
    10441 aggtaaccct cccggttcgc atgaaggggt cagtctggac gagagataac ccggcagcgt
    10501 gtcacatgtc agcggcttgt aacctgatgg aattgaatcc gtcataacag ttctccttgt
    10561 ctacacagaa tgataatcaa cttgtcatgt gacaactata cattatcaaa tgtaaaatta
//
LOCUS       DACADA010000150        10450 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.152, whole genome shotgun sequence.
ACCESSION   DACADA010000150 DACADA010000000
VERSION     DACADA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.152"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..499
                     /locus_tag="ISB17_003838"
     CDS             <1..499
                     /locus_tag="ISB17_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP1981721.1"
                     /translation="QCALLPLSKLIIISDNFRKLAKNADYNGPELFTHAHQFVGQNYL
                     GFGDYTITGQVLDLGGGQPSAVAAHLVFKNLQANTVWIRHFVSSNTQRGSSNVTAKFL
                     DVSDQITNLVPQHPTQFGSNIGLNYYYYNSQPTVRHFPGLPKNKQYQITHHICFMLDL
                     IAGRI"
     gene            complement(479..1654)
                     /locus_tag="ISB17_003839"
     CDS             complement(479..1654)
                     /locus_tag="ISB17_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1981722.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTAGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARTKRDEARSMLANGINPSEARKADKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPEFVNA
                     LDNYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAINILKKLQEITGNYSLVFPGRNDVSKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYIKDRQVMMEWYASLLNTTCY"
     gene            complement(1815..1889)
                     /locus_tag="ISB17_003840"
     tRNA            complement(1815..1889)
                     /locus_tag="ISB17_003840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1854..1856),aa:Arg,seq:cct)
     gene            complement(1965..2897)
                     /locus_tag="ISB17_003841"
     CDS             complement(1965..2897)
                     /locus_tag="ISB17_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP1981723.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3191..3946
                     /gene="mlaA"
                     /locus_tag="ISB17_003842"
     CDS             3191..3946
                     /gene="mlaA"
                     /locus_tag="ISB17_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAP1981724.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4008..5348)
                     /gene="fadL"
                     /locus_tag="ISB17_003843"
     CDS             complement(4008..5348)
                     /gene="fadL"
                     /locus_tag="ISB17_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAP1981725.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            5720..6004
                     /locus_tag="ISB17_003844"
     CDS             5720..6004
                     /locus_tag="ISB17_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAP1981726.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6185..7495
                     /gene="fadI"
                     /locus_tag="ISB17_003845"
     CDS             6185..7495
                     /gene="fadI"
                     /locus_tag="ISB17_003845"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAP1981727.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7495..9639
                     /gene="fadJ"
                     /locus_tag="ISB17_003846"
     CDS             7495..9639
                     /gene="fadJ"
                     /locus_tag="ISB17_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAP1981728.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            9842..10327
                     /gene="sixA"
                     /locus_tag="ISB17_003847"
     CDS             9842..10327
                     /gene="sixA"
                     /locus_tag="ISB17_003847"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAP1981729.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2590 a         2474 c         2626 g         2760 t
ORIGIN      
        1 tcagtgcgct cttcttcctt tgtcgaagtt aattattatt agcgataact ttagaaagtt
       61 agcaaaaaat gctgattata atgggccgga acttttcacg cacgcacatc aattcgtggg
      121 acaaaattac ttaggatttg gcgattatac aattactggc caggtactag atcttggagg
      181 tggtcaacct tctgctgttg ccgcacattt ggtgttcaag aatttacaag cgaatacagt
      241 ttggattcgt cattttgtat caagcaacac gcaacgagga agttcaaatg ttactgcaaa
      301 atttttagat gtttcagatc aaattacaaa tcttgttccc caacatccaa cccagtttgg
      361 cagcaacatt ggtcttaact attattatta caatagtcaa ccaactgtaa ggcatttccc
      421 tgggttgcca aaaaacaaac agtatcaaat tactcaccat atttgtttca tgctggattt
      481 aatagcaggt cgtatttaat aagctagcgt accattccat cattacttgt cgatctttaa
      541 tatattgcgc atgattgtaa gtacctcgaa tagagttctt atcaacatgc gcaagctgca
      601 tttcaatcca ggcactttca aacccatgct catgcaagat ggtgctcatg gtatgcctga
      661 aaccgtgacc cgttaaccga ccgtgataac ccagtagttt tatcactttg ttgatgctgg
      721 cttcgctcat tggtttactg acgtcattcc ggccagggaa aacgaggctg taattccctg
      781 ttatttcttg cagctttttc aggatattga tcgcctgagt tgataacgga accagatggg
      841 ggcggcgttt cttcatccgt tctttgggga tctcccataa ggcattatct aaatcaaact
      901 cagcccattc cgctgcacgt agttcaattg ttcgcacgcc tgttagcatc aacagctgcg
      961 tggcgtattt ggtaactaaa ctgccctgat agttatccag agcattaacg aactcgggca
     1021 actcgctttc agtcaggaac gggaagtgtt gggttttagg tttgttgagt gcgatagcta
     1081 aatcgggtgc aaaattatac tttgctctac cagtggcaac agcataacga agcacttcac
     1141 cacagcggcg gcggatctta ctggtttgct ctagcgcacc tcttttctct attttttgca
     1201 ggacggttag cagttctaat ggttcaatct gctcaattgg gcgctggccg ataaaaggaa
     1261 aaatatcctt ttccatacaa ttgagaacct cttttgcata accctctgac caggtggctt
     1321 ttttcgatga gtgccattct ctggccacag actcaaagct gttctcatgc gcgaactgca
     1381 aagcaatctt atctgctttt ctggcttcac ttgggtttat cccgttggct aacatcgaac
     1441 gcgcttcatc acgtttggta cgtgcctgtg ccaacgacac atcgccatat acgccaaagg
     1501 aaatcatctt aggcttacct gcaaagcgat agcgaaaacg ccagccttta ctgcccgctg
     1561 tgtcgatcag gagtgatagc cccataccat cgttaagcgt atagggtttg tccttcggtt
     1621 ttgctctttt tatttggatg tcagatagca gcatgtgtat agaaaaaaga tcgaactcag
     1681 ttatacacaa tgttatacgc aaagatgtat agattccatt agaaataatg gtactttgct
     1741 ggacgagtat aaggagatga tatcagttaa aacagtgagt tatggaatta attgaactta
     1801 gtgagaagtg ttcatggtgt cccctgcagg aatcgaacct gcaattagcc cttaggaggg
     1861 gctcgttata tccatttaac taagaggaca atgcggcatg agtatacccg ctaatggact
     1921 acggggtaag tacacagccg ctcgattgct taaaccctcg ccatttatgc cgggttttta
     1981 tcatttttct taatgttttc cgcacgttct gctttttgac gtgcttctgc tttgcgcttg
     2041 ttgctcatgt cgttacgaat ctgtgcatga ctcattaacg cgaagataaa ggtgccgccg
     2101 cagatgttcc ccgctaaagt aggtagtgcg aagggccaga tgaaatcgct ccagtgcagc
     2161 gtgccgttaa acaccagata gaggatttca acagaaccga caacgatgtg ggtggtgtca
     2221 cccagggcaa taagccaggt catcaatata atcaccacaa tctttgccgc acccgctgca
     2281 ggaaacatcc ataccatagt ggcgatcagc cagccggaaa taatcgcgtt ggcaaacatc
     2341 tcgctggggg tgttcttcat cacatccatg ccgattttga caaatgcatc gcgagtttct
     2401 tcattgaaga taggcatata ttcaaatgcc cacgcagcaa tacctgtccc gagaatatta
     2461 cccagcagca cgatgcccca taaccgcata agtaagccga cgttgctcat tgtcggtttt
     2521 tgcatgacgg gtagtaccgc agttacggtg ttttcggtaa ataattgctg gcgggccatt
     2581 atgacgataa taaaaccaaa ggtataaccg agattctcca gcaagaagct gcccggcacg
     2641 ccttccagtt cgacatgaaa tatccctttt gccagtaacg aagcgcccat cgacaggccc
     2701 gccgcaatgg ctgaccacag tagcgccatt gcgtcgcgtt ccagctcttt ttcaccatcc
     2761 tggcggatat gctcatgaat tgccatcgcc cgggagggga gtcggtcttc atctatttct
     2821 atttttttac cgcgctcttt ttcttcgctc tcaacttcaa tttcgtcgct gtgttgatca
     2881 attttgtcgt tgtccatggt ctcttctctt gaattagcac gtatagctaa agcgtagcgg
     2941 cttttttgct cgcaactggc gggagttact ctgaaaatgt agaaaaggcc gcgtttgcct
     3001 ttttctgttt ctattgaatc aagtagccta cagggcggcg attaccaggc tatgatcaaa
     3061 tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt tacaatcgcc cacacctaaa
     3121 caggcggata cggtatcgtt ccgtcatgga tggcaaactg cataagccat aaaaaaacag
     3181 ggagacattt atgaagcttc gcctgtcggc gcttgctctg ggaactacgc ttctggtggg
     3241 gtgtgcgagt tccggtacag atcagcaagg gcgttctgac ccgttagaag ggtttaaccg
     3301 cactatgtac aacttcaact tcaatgtatt agacccgtat attgttcgac cggtcgctgt
     3361 cgcctggcgt gattatgttc cgcaacctgc gcgtaacggt ttgagcaact ttaccggcaa
     3421 ccttgaagaa cctgcggtga tggttaacta cttcttgcag ggcgaccctt atcaggggat
     3481 ggtccacttt acccgctttt tcctgaatac cattttggga atgggcggct ttattgatgt
     3541 tgcagggatg gcgaacccga aactgcaacg gactgaacct caccgcttcg gtagtacgct
     3601 tggtcattat ggtgtgggtt atgggccgta cgttcagtta ccgttctacg gtagcttcac
     3661 gctgcgtgat gacggtggtg atatggcgga tagtctttac ccggttcttt cctggctgac
     3721 ctggccgatg tctgtgggta aatggacgct tgaagggata gaaactcgtg cgcagttgtt
     3781 ggattccgac ggtctgctgc gtcagtcctc cgatccttat attatggtgc gtgaagcgta
     3841 cttccagcgt catgatttca tcgctaatgg cggtgaactc aaaccgcagg aaaatccgaa
     3901 cgcgcaagcg attcaggatg atttaaaaga tatcgattct gaataagaaa caaacaaaaa
     3961 aggtgagtcg caaaactcac cttttttctt attaagctaa cgcgttatca gaacgcgtag
     4021 ttaaagttag taccgaacag ccaggcttta ccttcagact cgaaggaata agctgccgct
     4081 ccttcacctt cggtgaattt cactttctga ccatgcatat aagatgcgcc aacatcaatg
     4141 gatgcatctt cattaaatgc gtaggttaac ccagcactta accacaaacg atcctggtca
     4201 ggaatggaaa tagaacgttt atctgccgga accgggctgt cgtcataagc gataccggta
     4261 cggaaggtcc agtttttatc ataatagtag gttgtaccca acgcgatacg atacgcgtcc
     4321 cggaatcctt catctttata gaacagagtc tgaccactat cgcctttcgc tttcaattcc
     4381 tggaactggc tccaactggt ataggccatg ctgtagtgaa ttgcccactg cggcgcaaca
     4441 cggttataac cagaaatctc ccacatctca ggcaggttca gcgttaagga tccattttgg
     4501 gttgccccca tcgtgccata cggtaagccc atgttaccca aaatattgtt cagatttggg
     4561 ttaatggagc ttttgtaatc cccatcgaag tcgattttaa cttctgaacg atatgtcagc
     4621 ccccaacggt tatctttatc cagttcataa agaatacccg cgttccagcc aaagccccat
     4681 tcgtcacctt tcaaatgagc aatttgagag tccggagtat taataccatt ggttaatgaa
     4741 gcgggtacac cctgggcaat caactgttga gacaacaatg gccccagcga accggcgtaa
     4801 cgctcaattt ttgccttggc ataaactgca tcaaaaccaa gaccaaaact ccagctattg
     4861 ttaaggcgat aagccccgct aaggttaagg ttgattgttt gcagatcggt tttaccgcca
     4921 tagataccag cagcgtagtt gtcggtaaat tccgtagcca ggccatagtt ggaagttatt
     4981 gacgctcccc aaccaaactg atcgttaata ggcgcgacaa agtgcaggtt aggtacccac
     5041 gctgtcggcg cgatattttt agcatttgca ctctgaccgg ttaaattaga atgtccggta
     5101 atatctacat ccggatcaat ataaaccgca cccgcagaaa atgtcgggcg gtcaaacatg
     5161 gtaatcaatg cggggttacg gctaacgtta cctgcatcat cggcaattgc accttcccct
     5221 gaataagccc ggcccaggcc agaggaagaa aattcgttta actgaaagcc tgccgaccag
     5281 gcctgggtgg agataagtgc cactgcgact gcgagagcag actttgtaaa cagggttttc
     5341 tggctcatga ccataacctc aatgatttat ttttatacaa actatgttac gtgctgtaac
     5401 aggagcgcga agtgtagggt ctgaggtaca tcttagaaat cagaccagtg gcgagagtat
     5461 aggtcggacc agctgggatg ttgcaagtat gtttctatct tttttcagat atgatctaag
     5521 aaacgcgatc cagctggcaa aattgggagg tggcgcactc atggattcgg gtgaattttg
     5581 ttttagatca tttttaagtg tgatttcggt cacttatccg attttgcaga attaacaact
     5641 ggagcagcac tttccggaag cgtaaaatat ccacaacgtt tatctggcac cttgggtgca
     5701 catacagagg aaatcaacga tgagtaaatg tagtgctgat gaaaccccgg tttgctgctg
     5761 tatggatgtt ggcaccatta tggacaactc cgactgcacc gcgtcttaca gccgtgtgtt
     5821 tgcaaaccgc gcagaagcag aacaaacgct ggcggcgctg actgaaaaag cccgtagcgt
     5881 ggaatccgaa ccgtgcaaaa tcaccccgac tttcactgaa gagtctgacg gtgtgcgcct
     5941 ggatatcgat ttcacttttg cctgcgaagc tgaaatgctg atcttccagc tgggtctgcg
     6001 ttaattcctc tcgggtacat acgcccctgt cagctgctga caggggcgtt ttattttctc
     6061 ctcctctttt ctctctgtgt catagctccc actttccctt ttctccactt ggctaaatgt
     6121 aaaaaaatgg ttaagactat gatcaggtca gaccacttta tttatttttt tacaggggag
     6181 tgttatgggt caggttttac cgctggttac ccgccagggc gatcgtatcg ccattgttag
     6241 cggtttacgt acgccttttg cccgacaggc gacggctttt catggcattc ccgcggttga
     6301 tttagggaag atggtggtag gcgaactgct ggcacgcacc gagatccccg ctgaagtgat
     6361 tgaacaattg gtcttcggtc aggttgtgca aatgccggaa gcgcccaaca ttgcccgtga
     6421 aattgttctt ggcactggaa tgaatgtgca tactgatgct tacagcgtca gccgcgcttg
     6481 cgccaccagt tttcaggcgg ttgcgaacgt tgccgaaagc ctgatggcgg gaactattcg
     6541 agcggggatt gccggtgggg cagattcctc ttccgtattg ccaattggcg tcagtaaaaa
     6601 actggcgcgc gtactggttg atgtcaacaa agcccgcaca atgagccagc gcctgaaact
     6661 cttctctcgc ctgcgtttgc gcgacttaat gcccgtaccg cctgcggtag cagaatattc
     6721 taccggcttg cggatggggg acaccgcaga gcaaatggcg aaaacctacg gcatcacccg
     6781 agaacagcaa gatgcactgg cgcaccgttc gcatcagcgt gccgctcagg cgtggtcaga
     6841 aggtaaactc aaagaagagg tgatgactgc ctttatccct ccttataaac aaccgcttgt
     6901 cgaagacaac aatattcgcg gtaattcctc tcttgctgat tacgcaaagt tgcgtccggc
     6961 gtttgatcgt aaacacggga cggtaacggc ggcgaacagt acgccgctga ccgatggcgc
     7021 ggcagcggtg atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg taccgctggg
     7081 ctatctgcgc agctacgcat ttactgcgat agatgtctgg caggacatgt tgctcggtcc
     7141 agcctggtca acaccgctgg cgctggaacg tgccggattg acgatgggcg atctgacatt
     7201 gatcgatatg cacgaagcct ttgccgccca gacgctggcc aatattcagt tgctgggtag
     7261 cgaacgtttt gctcgcgacg tactggggcg tgcacatgcc actggcgaag tggacgagag
     7321 caaatttaac gtgcttggcg gttcgattgc ttacgggcat cccttcgcag cgaccggcgc
     7381 gcggatgatt acccagacat tgcatgaact tcgccgtcgt ggcggtggat ttggtttagt
     7441 gaccgcctgt gctgcgggtg ggcttggcgc agcaatggtt ctggaggcgg aataatggaa
     7501 atggcatcag cgtttactct taatgttcgt ctggacaaca ttgctatcat caccatcgac
     7561 gtaccgggtg agaaaatgaa taccctgaag gcggaatttg cctcgcaggt acgcgccatt
     7621 attaagcaac tccgtgaaaa caaagagttg cgtggcgtgg tgtttgtctc cgcgaaacca
     7681 gacaacttca ttgctggcgc agacatcaac atgatcggca actgcaaaac ggcgcaagaa
     7741 gcggaagtac tggcgcggca ggggcaacag ttaatggcgg agattcatgc tttgcccatt
     7801 ccggttatcg cggctattca tggcgcttgc ctgggcggcg ggctggagtt ggcgctggcg
     7861 tgccacggtc gtgtttgtac tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa
     7921 cttggattgt tacccggttc aggaggtacc cagcgtttac cgcgcctgat aggtgtcagc
     7981 acagcattag agatgatcct caccggaaaa caacttcggg cgaaacaggc ggtaaagctg
     8041 gggttggtgg atgacgtcgt tccgcactcc atattgctgg aagccgctgt tgagctggca
     8101 aagcaggatc gcccatcttc acgccctcta cctgttcgcg agcgtattct ggcggggccg
     8161 ttaggtcgtg cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa aacgcaaggt
     8221 aactatccgg cgacagaacg catcctggag gttgtcgaaa cgggattagc gcaggggacc
     8281 agcagtggct atgacgccga agctcgggcg tttggtgaac tggcgatgac gccgcaatcg
     8341 caggcgctgc gcaatatctt ttttgccagt acggacgtga aaaaagatcc cggcagtgat
     8401 gcgccgcctg cgccattaaa cagcgtgggg attctaggtg gtggcttgat gggcggcggt
     8461 attgcttatg tcactgcttg taaagcgggg cttccggtca gaattaaaga tatcaacccg
     8521 cagggcataa atcatgcgct gaagttcagt tgggatcagc ttgagggcaa agttcgccgt
     8581 cgtcatctca aagccagcga acgtgacaaa cagctggcat taatctccgg aacgacggac
     8641 tattgcggct ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa tctcgaatta
     8701 aaacaacaga tggtggcgga agtggagcaa aattgcgcta ctcataccat ctttgcttcg
     8761 aacacgtcat ctttaccgat tggcgatatc gccgctcacg ccacgcgacc tgagcaagtt
     8821 attggcctgc atttcttcag tccggtggaa aaaatgccgc tggtggagat tattcctcat
     8881 gcgagtacat cggcgcaaac catcgccacc acggtaaaac tggcgaaaaa acagggcaaa
     8941 atgccaattg tcgtgcgtga caaagctggg ttttacgtca atcgcatctt agcgccttac
     9001 atcaatgaag ctatccgcat gttgaccgaa ggcgagcgta ttgagcatat cgatgccgcg
     9061 ctagtgaaat ttggttttcc ggtaggccca atccaacttt tggatgaggt aggaatcgac
     9121 acagggacta aaattatgcc tgtactggaa gccgcttatg gagaacgttt tagcgcgtct
     9181 gcaaatgttg tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt
     9241 ttctatcttt atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc cgccatttac
     9301 ccgctcattg gcgtacaagg gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt
     9361 gtgatgttga tgctgaatga agcagtacgt tgtctggacg agcaggttat ccgtagcgtg
     9421 cgtgacgggg atattggtgc ggtatttggc attggttttc cgccatttct cggtggaccg
     9481 ttccgctata tcgattctct cggcgcaggc gaagtggttg caataatgca acgactggcc
     9541 acgcagtatg gttcccgttt taccccttgc gatcgtttag ttgagatgag cgagcgtggg
     9601 gaaagttttt ggaaaacaac tgcaactgac ctgcaataag aaggtcaaag ttatatgaat
     9661 ccgcgctgaa tggcggagtg ttgctcaaaa tgtaaacgca tattgactat acttacgcca
     9721 ttgaggtaaa aaacagcgtt tcattcggtg aatggataag gcacaatgcc ggccaccgtt
     9781 ttctttctct ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa agcggtgcaa
     9841 tatgcaagtt tttatcatgc gtcacggcga cgcagccctc gatgccgcca gtgattcggt
     9901 tcgtcctctg accactcatg gttgtgacga atctcgcctg atggcgaact ggctgaaagg
     9961 tcaaaaagtg gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg agcaaacact
    10021 tgaagaagta ggggattgtc tgaacctgcc ttccagtgca gaagttctgc cggagttaac
    10081 gccctgcggc gatgttggtc tggtcggtgc ctatttgcag gcgttgacca atgaaggtgt
    10141 tgcttcggta ctggtgatct cccacttacc gttagttggg tatctggttg ccgagttatg
    10201 tccgggcgaa acgccgccta tgtttaccac gtcagcgatt gccagcgtaa cgcttgatga
    10261 aagcggaaaa ggccaattca actggcagat gagcccttgt aatttgaaga tggcaaaagc
    10321 aatttaagca atcaatgccc ggttacgtgc gcgtacaccg ggcagtttct ccgtcataca
    10381 atcattcttt ttttgtctaa atgttgttaa tatttaacaa ctctcgatag ttattttccc
    10441 attattgaat
//
LOCUS       DACADA010000151        10399 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.153, whole genome shotgun sequence.
ACCESSION   DACADA010000151 DACADA010000000
VERSION     DACADA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.153"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            99..2240
                     /locus_tag="ISB17_003848"
     CDS             99..2240
                     /locus_tag="ISB17_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HAP1981730.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2304..4196)
                     /gene="parE"
                     /locus_tag="ISB17_003849"
     CDS             complement(2304..4196)
                     /gene="parE"
                     /locus_tag="ISB17_003849"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP1981731.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRLDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4225..4806)
                     /gene="yqiA"
                     /locus_tag="ISB17_003850"
     CDS             complement(4225..4806)
                     /gene="yqiA"
                     /locus_tag="ISB17_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAP1981732.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4806..5633)
                     /gene="cpdA"
                     /locus_tag="ISB17_003851"
     CDS             complement(4806..5633)
                     /gene="cpdA"
                     /locus_tag="ISB17_003851"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAP1981733.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5658..6080)
                     /locus_tag="ISB17_003852"
     CDS             complement(5658..6080)
                     /locus_tag="ISB17_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAP1981734.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6081..6710)
                     /gene="nudF"
                     /locus_tag="ISB17_003853"
     CDS             complement(6081..6710)
                     /gene="nudF"
                     /locus_tag="ISB17_003853"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAP1981735.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6915..8396
                     /gene="tolC"
                     /locus_tag="ISB17_003854"
     CDS             6915..8396
                     /gene="tolC"
                     /locus_tag="ISB17_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAP1981736.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8544..9215
                     /locus_tag="ISB17_003855"
     CDS             8544..9215
                     /locus_tag="ISB17_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAP1981737.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9221..10381
                     /locus_tag="ISB17_003856"
     CDS             9221..10381
                     /locus_tag="ISB17_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP1981738.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2616 a         2765 c         2581 g         2437 t
ORIGIN      
        1 atatccgatg gcgaaataac aaacggatag tgcttagtga gcaataagaa acaattttca
       61 ctcgcgacaa tccgcgagtt tttgggataa cagactaaat ggctatgttc acaccttcat
      121 tctcaggact caaaggtcgg gcgctctttt cactgctttt tgcggcaccg atgattcatg
      181 caacagactc tgtaacgacc aaagatggcg aaacaatcac tgttacagca gatgcaaata
      241 ccgcaactga ggcaaccgat ggttatcaac ctctgagcac ctctacggcg acattaaccg
      301 atatgccgat gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa
      361 accagaatgc gacaacgctg gatgaggcgc tttataacgt cagtaacgtg gtacagacca
      421 atacattagg cggaactcag gacgcctttg tacgccgtgg gtttggcgca aaccgggatg
      481 gctccatcat gaccaacggt ctgcgaaccg tacttcctcg cagtttcaac gccgccacag
      541 aacgtgtgga agtgctaaaa ggcccggcct ccacgctgta tggcattctc gatcctggcg
      601 gactgattaa cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttcg gtttcagcca
      661 cctcctccag ttttggtggc ggcactgggc aacttgatat cacaggtccc attgaaggca
      721 ctcagctggc gtatcgcctt accggggaag tgcaggatga agattactgg cgaaacttcg
      781 gtaaagagcg cagtacattt attgccccgt cactcacatg gtttggtgat aatgcaacag
      841 taaccatgct ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg
      901 accttacgac gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta
      961 atattacaga tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc
     1021 agtggacagc acgctttgat tacagctaca gccaggataa atacagcgat aatcaggcgc
     1081 gtgttaccgc atatgatgca acgacaggaa cactgacacg gcgtgttgat gcaactcagg
     1141 gctctaccca gcgtatgcat gctactcgtg cggatctgca agggaatgtt gatattgccg
     1201 gattctataa tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgtacag
     1261 atatgattcg ctgtaaaaaa gctaaagatt tcaatattta caaccctgtt tatggtaata
     1321 ccagcaaatg tacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct
     1381 actcagcgta tgcacaggac gcactctatc tgaccgataa ctggattgcc gtcgccggga
     1441 tccgctatca gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg
     1501 acagccgcga tgaacaatgg actcccaaac tggggttggt ctacaaactg acgccatcgg
     1561 tatccttatt tgccaattat tcgcaaacat ttatgccgca gtcgtcaatt gccagctaca
     1621 ttggcgatct tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctat
     1681 tcgatggtat caccgcagat attgcgctgt ttgatatcca taaacgtaat gtgttgtata
     1741 ccgaaagtat tggtgatgaa accatcgcca aaacggcagg ccgtgttcgt tcaagagggg
     1801 tagaagtcga ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct
     1861 ataccgatgc taaggttctg gaggatcctg attatgcagg gaaaccattg ccgaatgttc
     1921 ctcgtcatac cggttcgcta ttcctgacct atgatattca taacatgcca ggcaataata
     1981 cgctgacatt tggcggtggt ggacatggtg taagccgtcg ttcggcaacc aatggggctg
     2041 actattatct gccaggctat tttgttgccg atgccttcgc cgcatacaaa atgaaattgc
     2101 agtatccggt cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct
     2161 cttccatcgc cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca
     2221 cggtgaaaat ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt
     2281 ttattcttct ggcgggagga ttatcagacc tcaatctctg ccatgtcgcc tttttcttgc
     2341 aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca
     2401 gtacgctgat cgtcttcatc atcgatagtc aactgcacca gacggcgagt gttcggatca
     2461 agcgtggttt cgcgcaattg catcgggttc atttccccga gacctttaaa acgctggacg
     2521 tttggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc
     2581 agcgcgtaat aaacctcttt cccgaggtca agacggtaga gcggtggcag tgcaacgtaa
     2641 acgtgaccgt gtttcaccaa tgcgcggaag tgttttacaa acaaagcgca gagcagcgtg
     2701 gcaatgtgca gaccatcgga gtcagcatcc gccaggatac agatcttgcc gtaacgaagc
     2761 tggctcaaat cgtcgctgtc aggatcgata ccgatagcta ccgaaatatc gtgcacttcc
     2821 tgcgaagcca gcacttcgtc ggaagagact tcccaggtgt taaggatctt acctttcagt
     2881 ggcatgatcg cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca
     2941 ccttccacaa ggaatagttc ggtacggtta agatcctgcg cggtacagtc agccaattta
     3001 ccaggcagcg ccgggccgct ggttagcttt ttacgcacca cttttttagc cgcacgcata
     3061 cgacgctggg cgctggaaat cgccatctct gccaacagct ccgccgcctg aacgttttgg
     3121 ttcagccaca ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc acactgacgc
     3181 gaagagagac gttctttggt ctgcccggca aactgcggat cctgcatttt tactgacagc
     3241 acataggcgc agcgatccca gatatcttcc gccgacagct ttacaccacg cggcagaatg
     3301 ttgcggtatt cacagaactc acgcatcgcg tccagcaggc cctgacgcag gccattaaca
     3361 tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg
     3421 ccttccggca gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg
     3481 ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc
     3541 agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaagtgatc
     3601 tcaacgcccg ggcacagtac cgctttggct ttcagcacat gcgtcaggcg tgaaacagaa
     3661 aaacgcgggc tgtcaaagaa ggtttcatcc ggccagaagt gcacgctggt accggtattg
     3721 cgtttaccgc aagtgccgac aacctgtaaa tcttgcacct tttcgccatt ttcaaaggcg
     3781 atgttataga tctgaccatc gcggcgtacg ttaacttcta cgcgcttcga cagggcgtta
     3841 actaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt gttagagaat
     3901 ttaccgcccg cgtgcagacg gcaaagaatc agttcaaccg ccggtacccc ctcttccggg
     3961 tgaatatcta ccggcatccc gcgcccatcg tcaatcactt ctaacgactg gtcggcatgt
     4021 aagattacat ccacgcgttt tgcgtggccc gccagtgctt catcgacact gttatcaata
     4081 acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga
     4141 accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt
     4201 taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg
     4261 gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg
     4321 gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg
     4381 cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt tgagatcgta
     4441 aatatggcgt gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag
     4501 atagtccgtc agcagttcaa acgggcgcac cgccgggttg accaccactg cgggcagcat
     4561 aaaacattgc gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag
     4621 cgaatcgccg ccatgttcca ggacaatgga ttccagcagt tctgccgcgt cggaaggata
     4681 cggcggcagc tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt
     4741 taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt
     4801 agacatcagt agccttctga agcggtatca ggttggaagc gtgcgtccgc caggcgatgc
     4861 acctcggtgg tcagcgtgcc atcagcatgt aactcaagag tacgccagcc gggcgcgatg
     4921 gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga cggcgttgcc
     4981 agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag
     5041 tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg
     5101 tgttgatcaa gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta
     5161 tggcgttctg gcgcatcggc cagtttacgt tccagccact caagctgaaa ctcgctcagc
     5221 tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca
     5281 ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcactgta catcgcgggc
     5341 tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc gatgccttca
     5401 gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc
     5461 aggtcgaatt cgtgctggtg tggccgaatc gcctccagca ccgcctggta gctttcccag
     5521 gtgtttaccc ctaacagggc ttcgtgcttt tgtgcaaaca ggtgagtgtc ggtaatttgt
     5581 aaaatcctga ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt
     5641 ccttaggttt cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat
     5701 cgcaaccagt ccgctaaaaa ctgattaatt tgatgctttt cgtcgcgttg atgcaacttt
     5761 ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca
     5821 gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc
     5881 gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc
     5941 agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc
     6001 ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag
     6061 tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc
     6121 agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg
     6181 tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg
     6241 tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg
     6301 gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagtccc
     6361 gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatc
     6421 ccggcaacca tctccagtag ccaaggggtt tcgctggtgt cgtacgcggc aatccgaatc
     6481 tgctcaatca gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga
     6541 ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga
     6601 cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt
     6661 tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc
     6721 ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt
     6781 catgtgttaa attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta
     6841 gcggcttctg ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac
     6901 cacaaggaat gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctctctgggt
     6961 tcagttcgtt gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta
     7021 acccggaatt gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag
     7081 cgcgcagtcc attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct
     7141 accgcgacgc gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat
     7201 ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gctgggattc
     7261 aggacgtcac gtatcagacc gatcaacaaa ccttgatcct caacaccgcg accgcttatt
     7321 tcaacgtgtt gaatgcgatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct
     7381 accgtcaatt agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcaccgacg
     7441 tgcagaacgc ccgcgcacag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata
     7501 accttgataa cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg
     7561 cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac acgctgctga
     7621 aagaagccga aaaacgcaac ctgtccctgt tacaggcgcg tttgagccag gacctggcgc
     7681 gcgagcaaat tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta
     7741 ccgggatttc tgacacctct tatagcggtt cgaaaacccg tggtgccgct ggtacccagt
     7801 atgacgatag caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc
     7861 agggcggaat ggttaactcg caggtgaaac aggcccagta caactttgtt ggtgccagcg
     7921 agcaactgga aagcgcgcat cgtagcgtcg tacaaaccgt acgttcctcc ttcaacaaca
     7981 ttaatgcttc tatcagtagc attaacgcct acaaacaagc cgtagtttcc gctcaaagct
     8041 cattagacgc gatggaggcg ggttactcgg tcggtacgcg taccattgtt gatgtgttgg
     8101 atgcgaccac cacgctgtac aacgctaagc aagagctggc aaatgcgcgt tataactacc
     8161 tgattaacca gttgaatatt aagtcagctc tgggtacgtt gaacgagcag gatctgctgg
     8221 cactgaacaa tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa
     8281 cgccggaaca gaatgctatt gctgatggtt atgcgcctga tagcccggca ccagtcgttc
     8341 agcaaacatc cgcacgcact accaccagta acggtcataa ccctttccgt aactgacgac
     8401 gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc
     8461 gcattcttcc cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt
     8521 accactggtc ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc
     8581 gcaaaaactg gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta
     8641 tgctggcggg ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact
     8701 gttcagctgc aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga
     8761 aagaagccga acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg
     8821 gtgaaggtca gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc
     8881 aggcccagca atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggac
     8941 gtctgatggg cggcggcgcg ggatttgcac agcagccgct gttctcctcg aaaaacccgg
     9001 ccagcccggc ttacggtaaa tataccgacg cgaccggtaa aaactatggc gcagcccagc
     9061 caggccgcac catgaccgta ccgaagacgg caatggcacc aaaaccggcg accactacta
     9121 ctgttacccg tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg
     9181 caaccggcac ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac
     9241 cgagcgcccg gactggcgcg aaaaagccca cgaatacggt ttcaattttc acaccatgta
     9301 cggcgagccg tactggtgtg aagatgctta ctacaagttg accctctctc aggttgaaaa
     9361 gctggaagaa gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat
     9421 cgccagcgat gagctgatga ccaaattccg cataccaaaa cacacctgga gttttgtgcg
     9481 ccagtcatgg ctgacgcacc agccatcgct ttattcgcgt cttgatctgg cgtgggatgg
     9541 cactggcgaa cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tatacgaggc
     9601 ggcgttcttt cagtggatct ggctggaaga tcagcttaac gccggtaact tgccggaggg
     9661 cagcgaccag tttaacagtc tgcaagaaaa gctgatcgat cgcttcgttg agctgcgtga
     9721 acagtatggc ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg
     9781 aaccattcag tatttgcagg actgcgcaac ggaagctgaa atcgccaccg agttcctcta
     9841 cattgacgat atcgggttag gtgaaaaagg tcagttcacg gatttacagg atcaggtgat
     9901 ttccaacctg ttcaagctgt atccgtggga atttatgttg cgtgagatgt tctccaccaa
     9961 gctggaggat gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa
    10021 agcgcttcta ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcccgctta
    10081 ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg
    10141 tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc
    10201 gtatggcgaa gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag
    10261 ctatatgctg attggtagct ggctggtgaa cgatcaacct gccggaattg gcattcgtga
    10321 agaccgtgca ttgatcaccc aggatatgtc tcggttttat ccacatattt ttgttgagta
    10381 atacagctta atattgccc
//
LOCUS       DACADA010000152        10322 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.154, whole genome shotgun sequence.
ACCESSION   DACADA010000152 DACADA010000000
VERSION     DACADA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.154"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            38..1315
                     /locus_tag="ISB17_003857"
     CDS             38..1315
                     /locus_tag="ISB17_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981739.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            1342..2820
                     /locus_tag="ISB17_003858"
     CDS             1342..2820
                     /locus_tag="ISB17_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1981740.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            2985..3905
                     /locus_tag="ISB17_003859"
     CDS             2985..3905
                     /locus_tag="ISB17_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAP1981741.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            4073..5590
                     /locus_tag="ISB17_003860"
     CDS             4073..5590
                     /locus_tag="ISB17_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP1981742.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            5651..7021
                     /locus_tag="ISB17_003861"
     CDS             5651..7021
                     /locus_tag="ISB17_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HAP1981743.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            7021..8430
                     /locus_tag="ISB17_003862"
     CDS             7021..8430
                     /locus_tag="ISB17_003862"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP1981744.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            8456..9463
                     /locus_tag="ISB17_003863"
     CDS             8456..9463
                     /locus_tag="ISB17_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1981745.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(9548..10219)
                     /gene="queE"
                     /locus_tag="ISB17_003864"
     CDS             complement(9548..10219)
                     /gene="queE"
                     /locus_tag="ISB17_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAP1981746.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         2627 a         2366 c         2541 g         2788 t
ORIGIN      
        1 ttctcttttt tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg
       61 ttggataacc ctcgcgataa ttagttttag cggcggcgtt agtttcgacc tggcttattt
      121 acgttatatt tatcaaattc ccatggcaaa atttatggga ttcagcaata ccgagatagg
      181 tttaataatg agtacctttg gtattgcggc cattattttc tatgccccta gcggcgttat
      241 tgccgataaa ttttcacatc gcaaaatgat tacttccgcg atgatcatta ccggattact
      301 gggtctgtta atggcaacat atccaccgct gtgggtaatg ctctgtattc aggtcgcctt
      361 tgcgataacg acgattttaa tgctgtggtc ggtgtcgatt aaagccgcat cgttgctggg
      421 cgatcatagc gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt
      481 aatgtcgctg gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc
      541 cagcctgaaa acggtcatta ttatctatag tgtggtttac attctgctgg gcattttgtg
      601 ttggtttttt gttagcgata acaacaacct acgcagtgcc aataacgaag aaaaacagtc
      661 attccagctt agcgacatcc tggccgtttt gcgcatcagc actacctggt attgcagcat
      721 ggtgattttt ggtgtcttca ccatctacgc cattctgagt tactccacca actatctgac
      781 cgaaatgtac ggcatgtcgc tggtggcggc gagctacatg gggattgtga tcaacaaaat
      841 cttccgcgcg ctgtgcggcc cacttggcgg cattatcacc acctacagta aagtgaaatc
      901 ccctacccgc gtggtccaaa tcctttccat catcggtttg ctggcgttga cagccctgct
      961 cgtcacgaac tctaacccgc aatcggtcgc gatggggatt ggcttgattt tattactggg
     1021 attcacctgt tacgcctcac gcgggctgta ctgggcctgc cctggcgaag cgagaacacc
     1081 gtcttacatt atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt
     1141 cttcgtttac ccgattatcg gctactggca agacaccctg cccgctgcag aagcctatcg
     1201 caacatgtgg ctaatgggca tggcggcgct tggcatggtg atcatcttta cctttttgct
     1261 gttccaaaaa attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccag
     1321 gaaaattaca ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt
     1381 cagagcacaa aagtggtgat gtacgatctg gaaggtaatg tggtttgcga aggcaaaggc
     1441 ttattgcagc cgatgcacac gccagatgcc gataccgcag aacatcctga cgatgattta
     1501 tgggcatcac tatgttttgc cagtcacgat ttgatgagcc agtttgccgg gaataaagaa
     1561 gatattgtcg gcattggcct gggctcaatc cgttgctgcc gtgcgctatt gaaagccgat
     1621 ggtacgccag ccgcgccgtt gattagctgg caggatgcac gcgttacacg cccttacgaa
     1681 cacactaatc ctgacgtggc ctatgtcacc tctttttcgg gttatctgac acatcgctta
     1741 accggcgagt ttaaagacaa tatcgccaac tattttggtc agtggccggt ggattataag
     1801 agctgggcat ggagcgaaga tgctacggta atggaaaagt ttaatatccc ccgccagatg
     1861 ctttttgacg tgcaaatgcc gggcaccatt cttgggcata tcacaccaca agctgcgctg
     1921 gcgacacatt tcccggctgg gctgccggtt gtttgtacca ccagtgataa accggtagaa
     1981 gctctgggag ccgggttact ggatgatgaa acggcggtaa tttctcttgg cacctatatc
     2041 gcgttgatga tgaacggcaa agcactgccg aaagatccgg tggcgtactg gccgattatg
     2101 tcttctattc cgcaaacatt gctgtatgaa ggttacggta ttcgcaaagg catgtggacg
     2161 gtgagctggc tgcgcgacat gttaggcgag tcgttaattc aggatgccaa agcacaggat
     2221 ctttcaccgg aagatttgct caacaaaaaa gcgtcttgcg tgccgcctgg ctgtaatggt
     2281 ctgatgacgg tgctggactg gctgaccaat ccgtgggaac cgtacaaacg cgggattatg
     2341 atcggttttg attccagcat ggattacgca tggatatatc gttcgatact ggaaagcgtg
     2401 gcgctgacgc tgaagaataa ttacgacaat atgtgtcatg aaatgaatca ctttgcaaag
     2461 catgtgatca ttactggcgg cggttcgaac agcgatctgt ttatgcagat ttttgccgac
     2521 gtgttcaacc ttccggcacg ccgaaacgcc attaacggtt gtgcaagtct gggggctgcg
     2581 atcaatacgg cggtaggtct ggggctatac ccggattaca caacggctgt cgataaaatg
     2641 gttcgcgtga aagatatctt cataccgatt gagagcaatg ccaaacgcta cgacgcgatg
     2701 aataaaggca ttttcaaaga cctaaccaaa catactgatg tgatcctgaa aaaatcgtat
     2761 gaagtgatgc atggggaatt ggggaatgcg gattcgatcc agagctggtc gaatgcgtaa
     2821 gtgagataaa cagtgccggg tagatgatta atcccggcat attcacgttt ccactacaaa
     2881 ataccgggta accactgctg aatattgttc tctcctgagc agcgtttatg ctttaattgc
     2941 taaaccgatt taattagttt agcaattagt atattggagc ctttatgagc acaagagtat
     3001 gggtactcgg tgatgcggtt gttgatttat tacccgaaag ccaggggaga ctactacagt
     3061 gtcctggtgg ggcgcctgct aatgttgcag tcggtatcgc aaggctgggg gggaaaagtg
     3121 cctttattgg caaagttggc gatgatcctt tcggtcgctt tatgtatcaa acactgagta
     3181 cagaaaatgt tgatacacgt tatatgtctc ttgatcctca acatcgcacc tcaattgtgg
     3241 ctgtaggact tgatgagcaa ggagaaagaa actttacctt tatggtacgc ccaagtgccg
     3301 atcttttttt acaacctgat gaccttcctg catttgggcc gggtgaatgg ctccatcttt
     3361 gttccattgc gctcagtgca gaaccttccc gaagtaccgc atttctggct atggagaaaa
     3421 tacgtcaggc tggcggaaac atcagttttg atcccaatat ccgcagcgat ctctggcaga
     3481 gtgaagcgct gttaaggaaa taccttgatc gtgcgctttc gctggcgaat atcgctaaat
     3541 tgtccgaaga agagttgtta ttcatcagtg acgaaagcca ggttcagcaa ggcgcatatt
     3601 cattagtaca acgttatccg ttgactttat tgcttattac acaaggaaaa aatggcgtac
     3661 ttgtgtattt tcaggggcag tttatccact atcccgccaa acctgtttct gtcgtcgata
     3721 cgaccggggc aggagatgct tttgtcgctg gattacttgc cggtctggct gattctggaa
     3781 taccaacaaa taccagacag cttgaacgaa tcattgcaca agctcagatt tgtggtgctc
     3841 tggcgaccac ggctaaaggc gcgatgaccg ccttaccccg acaacacgat ctcccttcac
     3901 aatagtgatc ctgctgttgt atctgtaacc cgatttgtcg tgcctgtcac atttactaaa
     3961 tcggtttagc gaaaataatt gctctggcta aaaagtcagc tttattatca acacctaaac
     4021 cggtttagca ttttagcaat ttttataatt ctctgatggg acgcgacaaa ttatgtataa
     4081 aaaaacaact ttggcagtgt taattgcttt gctgaccggt gctacaacgg tacatgcgca
     4141 aacggatatt agcagtatcg aatctcgact ggcggcgttg gaacaacgtt taaaaaatgc
     4201 ggaatcccgc gcccaggcgg cagaagcaag ggccaaaaca gctgaattac aggttcagaa
     4261 actggctgaa acacaacaac aaaatcagct aacaactcaa gaagtagcac agagaacagt
     4321 tcagctcgaa cagaaatccg cagaaaacag tggttttgag tttcatggct atgcccgttc
     4381 cgggttactg atgaatgatg ccgcttccag tagcaaaagt gggccgtatc tgactcccgc
     4441 aggtgaaact ggtggagctg ttggccgtct gggaaatgaa gccgatacct atgtcgagtt
     4501 aaatgtagaa cataaacaaa cactggataa cggtgcgacc acacgcttta aagcaatgtt
     4561 ggctgacgga caaagagatt acaacgactg gactggcggc tccagtaacc tgaatatccg
     4621 acaggctttt gccgaactgg gcgcattacc aagttttacc ggagcattca aagacagtac
     4681 tgtctgggct ggtaaacgct ttgatcgcga caattttgat attcactggt tagactccga
     4741 tgtcgtattt ttagcgggaa cgggcggcgg tatctatgac gtaaaatgga acgagacatt
     4801 ccgcagtaac ttttctctct acggacgtaa tttcggcgat cttgatgata tcgacaataa
     4861 cgttcagaac tacatcctca ccatgaatca ttatgcaggc cccttccagt tgatggttag
     4921 cggattacgg gcaaaagata atgatgatcg aaaagatgcc aatggtgatc tcattcaaac
     4981 tgatgctgca aatactggcg tacatgcgtt agttggtctg cacaatgaca ctttctatgg
     5041 cctgcgtgaa gggacggcaa aaacagcact gctatatggc catggcctgg gtgcggaagt
     5101 caaagggatt ggctccgatg gcgctctgct gtctgaggcg aatacctggc gcttcgcatc
     5161 ttacggcaca acacctctgg gaagcggttg gtatgttgcg ccagcaattc tcgcacaaag
     5221 cagtaaagat cgttacgtca aaggcgatag ctacgaatgg gtgaccttca atacacgtct
     5281 gatcaaagag gtaacacaga attttgctct ggcctttgag ggtagctatc aatatatgga
     5341 tctgaagcca aaggggtatc aaaaccacaa cgccgtaaac ggcagcttct ataaactcac
     5401 ctttgctcca actctaaaag ctaacgatat caataatttc tttagccgtc cggagcttcg
     5461 cctgtttgcc acctggatgg actggagcag caaacttgat gattttgcca gcaatgatgc
     5521 tttcggcagc agtggtttca atactggcgg agagtggaat tttggtgtcc aaatggaaac
     5581 ctggttttaa taccagacag cctcacggat gtgaggcttt tactgaaata acaatgaatt
     5641 agagggttat atggatttcg ataaaatcgc ccaatcgttg cttcctttat taggtggtaa
     5701 ggagaacatc gccagtgcag cacattgcgc cactcgccta cggctggtac tggttgacga
     5761 cacacttgcc gatcaacatg ccattggcca gattgatgga gtgaaaggtt gttttcgtaa
     5821 ttcagggcaa atgcaaatca tctttggcac cggcgtggtt aataaagtct atgctgcctt
     5881 tattcaggtt gcaggtatta gcgaatccag taaagcagat accgctcgac tcgccgctca
     5941 aaaactcaat ccttttcagc gaatagcacg gctactttct aatatttttg tccccatcat
     6001 tccagccatt gttgcttctg gtttattaat ggggcttctg ggaatggtga aaacatatgg
     6061 ctgggtgaat gccgataatg cgatttatat cctgctggat atgtgcagtt cagccgcatt
     6121 tattattctc cccatcctta ttggctttac tgctgctcgt gaatttggtg gaaatcctta
     6181 tcttggcgcg acattaggag ggattctgac tcacccggca ctcacaaatg cctggggcgt
     6241 tgctgcggga tttcagacaa tgaacttctt cggttttgaa atcgccatga ttggctatca
     6301 gggaacggtt ttccccgtgc ttctggcagt atggtttatg agcatagtgg aaaaacagtt
     6361 acgcaggttt atccctgatg ctctggatct cattctgacg ccatttctga ctgtcgtcat
     6421 ttctggcttt atcgctcttt tgattattgg tccggcagga cgagcgttag gtgacggtat
     6481 ctctttcgtt ctcagcacgc tgattgcaca cgcaggctgg ttagcagggt tgttattcgg
     6541 cgggctatat tcagcgattg ttattacggg tattcatcat agcttccacg caattgaagc
     6601 aggactttta ggtaaccctg caatcggtgt taatttcctg ctgcctattt gggcaatggc
     6661 aaacgttgcg caaggcggtg catgtctggc ggtatggttt aaaaccaaag atacaaaaat
     6721 taaagcaatc accctaccct cggccttttc cgcaatgtta gggattactg aagccgctat
     6781 ttttggtatc aaccttcgtt ttgttaaacc ttttattgcg gccttaattg gtggcgctgc
     6841 cggcggtgcc tgggttgtct ctgttcacgt ttatatgact gccgttggtc tgaccgcaat
     6901 acccggcatg gcaatcgttc agccaacatc gttggttaat tatattattg gaatggtgat
     6961 tgctttcgct gtcgctttta gcctctcttt attactcaaa tacaaaacgg acgaggagta
     7021 atgtcgatgc cttcacaaat gcccgcgatt ttacaggcag tgatgaaagg tcagtccaaa
     7081 gcactggcag atgcccacta tccttgttgg catctcgctc cagtcaccgg actaatgaac
     7141 gatccaaatg gcttttgttg gtctggaggc cgctatcatc tgttttatca atggaatcca
     7201 ctggcttgtg atcataagta caaatgttgg gggcactgga gttcgacgga tttattgcac
     7261 tggcagcatg aaccattagc gctaatgccc gataaagagt acgaccgtaa tggttgttac
     7321 tcaggaagcg cggttaataa tcagggcgtt ctcacacttt gctataccgg taatgttaag
     7381 tttgatgatg gcagcagaac agcatggcaa tgtctggcca ccgaaaataa ccagggcggg
     7441 tttgataaac taggccctgt cataccgtta cctgatggtt acaccggaca tgttcgtgat
     7501 ccaaaagtct ggaagcacaa cagccaatgg tacatggtgc ttggcgcgca agataaagag
     7561 aaacggggga aagtactgct gtattcctct gtcgatctct atacctggtc ttttcatgga
     7621 gaaattgctg gtaatggtct gaatgaaata gataacgcag gatacatgtg ggaatgtcct
     7681 gatcttttcg cccttgatgg tgaatacatt ctcctctgct gcccccaggg aatggcgcgc
     7741 gaacatgagc gctatctgaa tacttatccc tgcgcctggc tacatggaca gtttgattac
     7801 gagacaggca agtttacgca tggcgctttc tcagagctgg atgcgggatt tgaattctat
     7861 gcaccacaga cgatggaagc cccggacggg cgacgattgc tggtcggctg gatgggggtt
     7921 cctgatggcg aagagatgtt gcaaccaacc agaaagcatc attggcagca tcaaatgacc
     7981 tgttttcgcg aactctcatt ccagaaggga aaattgtttc agatgccaat cagggaactc
     8041 gcgcaactgc gtgaagctga acacttctgg caaggaaaag cggatcatgc gccgcatgtc
     8101 gaaattgaac gcctggaaat ggacatcatt ccgtcgggtg agctgtatct gaattttggc
     8161 aatgcgctgg cactgcatct taatgacgat ggtattcaac tacagaggag aagccttgca
     8221 gggcaagaga aactaactcg ttactggcga ggaagcgtaa cgtcactgaa gattctctgt
     8281 gacagttcca gcgtagagat attcatcaat aatggtgaag gtgtaatgag taatcgttat
     8341 tttccccatc atccagcttc gctgatatta cagggtgagt ctgacgtcac gttacgctac
     8401 tggtcgctac gcgcctgcat ggtagaatga gggttttgaa ttacggatcc tcgtcgtgag
     8461 aaaaaccaaa cgcgttacta ttaaagacat tgctgaactg gcgggagtgt cgaaagcaac
     8521 cgccagtctg gttctcaatg gtcgtggcaa agagttacgt gttgcgcagg aaacgcgtga
     8581 gcgtgtgctg agtattgccc aacagcagca ttatcagccc agcattcatg cccgctcatt
     8641 acgggacaac cgcagtcata ccattggtct cgtcgttcct gaaatcacta actacggatt
     8701 tgcagtattt tcccatgaac tggaaacatt gtgtcgggaa gcgggcgtcc aactcctcat
     8761 atcctgtact gatgaaaatc cttcacagga aacaatggtg gtgaacaata tgattgcccg
     8821 ccaggttgat gggttaatcg tcgcatccag tatgctgcat gataatgact atcaaaaact
     8881 tagcgagcaa ttacctgttg tgttgttcga tcgccacatg aacggcagca cattgccatt
     8941 ggtcattact gactctgttt ctcccaccgc cgctctggtg gcagatattg cacggagtca
     9001 cccggatgaa ttctattttc tggggggaca accccgtttg tcaccgactc gtgatcgact
     9061 ggaaggattt acacaaggct tgcagcaagc cggagtaacg ctacaacctg aatggattat
     9121 tcacggtaat tatcatccga gctcaggcta tgaaatgttt gccgcgctat gcgctcgttt
     9181 aggacgtccc cctaaagcct tattcactgc cgcttgtgga ttactggaag gcgttctgcg
     9241 ctatatgagc caatataatt tactcgacag taaaatccac ctggcgagtt ttgatgatca
     9301 ttacttgtat gattcacttt cggtaagaat cgacacaata caacaggata atcgccaact
     9361 tgcttttcac tgctttgaac ttatttcgca actgattgaa ggtgaaacgc catccccatt
     9421 acagcgttat cttcctgcca gtctacaaaa acgctaccga taatcccgtc actatcttgc
     9481 atcgcccctg taaaacgggg cgattatttt catcctgaac gttaaatatc aatgatgcat
     9541 aatcaaatca ggcaatattt aaatatttat gcgtttgcat cgacaaacgc cagttgcgcg
     9601 caatgcaggt atcgatacat aaacgcgtag catcttcctt ctggctaatt ggctgcagtg
     9661 caatgactcg cggtttatca tcggtcagcg tcgcaagcaa ttcatccagt gcttcgatat
     9721 cacgtacgcg tcccactggg tgtttgattt cgttggctcg ctccagtgcc tgtgacaaca
     9781 cttcatagcc accgcgcata ttcagctttg gcgacacggt gacccaggta ttcggtgtac
     9841 agcgtacttc atgagtacca ctggtttcta tctggcagct aaaaccgttc ttttcgagca
     9901 ggtcagtcag cggcagcaaa tcatgaatgc aaggctcacc acccgtaatc accacgtgac
     9961 gcgcggtgta tccctgacgg ccaatgacag ccagcaaatc ttcactgctc gccgcccccc
    10021 acttatcact ctctttggtc ttcgccagaa tgctgaaaag ggagacttcc cgatcctcaa
    10081 gcttttccca ggtgtgtttg gtgtcgcacc aggcacagcc aaccggacat ccctgtaaac
    10141 gaataaaaat ggcggggacg ccggtaaagt aaccctcacc ttgcagggtc tggaacatct
    10201 cgttaatcgg gtactgcata gcattctctg tgaagtggat aattgttaat tattgcagat
    10261 cctgccacaa caatcatgtc ttattaacat tctgttaccg gcaggtttaa agaaaaatgc
    10321 ga
//
LOCUS       DACADA010000153        10300 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.155, whole genome shotgun sequence.
ACCESSION   DACADA010000153 DACADA010000000
VERSION     DACADA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.155"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..1806)
                     /gene="selB"
                     /locus_tag="ISB17_003865"
     CDS             complement(<1..1806)
                     /gene="selB"
                     /locus_tag="ISB17_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAP1981747.1"
                     /translation="MIITTAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
     gene            complement(1803..3194)
                     /gene="selA"
                     /locus_tag="ISB17_003866"
     CDS             complement(1803..3194)
                     /gene="selA"
                     /locus_tag="ISB17_003866"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAP1981748.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3292..3900)
                     /locus_tag="ISB17_003867"
     CDS             complement(3292..3900)
                     /locus_tag="ISB17_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP1981749.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3971..5107)
                     /locus_tag="ISB17_003868"
     CDS             complement(3971..5107)
                     /locus_tag="ISB17_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1981750.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLEPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5110..5472)
                     /locus_tag="ISB17_003869"
     CDS             complement(5110..5472)
                     /locus_tag="ISB17_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAP1981751.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6009..7922
                     /locus_tag="ISB17_003870"
     CDS             6009..7922
                     /locus_tag="ISB17_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP1981752.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8242..9390
                     /gene="mtlD"
                     /locus_tag="ISB17_003871"
     CDS             8242..9390
                     /gene="mtlD"
                     /locus_tag="ISB17_003871"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP1981753.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9390..9977
                     /gene="mtlR"
                     /locus_tag="ISB17_003872"
     CDS             9390..9977
                     /gene="mtlR"
                     /locus_tag="ISB17_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAP1981754.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9988..10197)
                     /locus_tag="ISB17_003873"
     CDS             complement(9988..10197)
                     /locus_tag="ISB17_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981755.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
BASE COUNT         2425 a         2819 c         2666 g         2390 t
ORIGIN      
        1 atcatttcca cgacgacgcg taaagccaat gcggtcaaaa tattccagaa tctgaattgc
       61 cagcttgcgg cctacgccta agcgatcgcg gaaatccgcc gcgcaggttg aaccacattc
      121 ctgatcgaga tcgcggatca tattggcaaa ttcgacaatc cgatcgttac ggtaataacg
      181 atctttgacg atcgcagtaa ttatcccctg ttgggccgcc tggcgtagcg tcaggcgcat
      241 tgcctgctcg tcggttcccg tctcttttgc caggtcacgc acccaccatg gctcgtcacc
      301 aaacagcggc tctgcttttt gccagatggc ctgctgctct tcgctgaagc ccgctttatg
      361 atccggcaga tgcagccagc cgtgatggct gtggatgtcg ccgctttcgc gcatcttttc
      421 aatcagcaac agtaccagcg cttcatcttc cattggcaac gccatacgtc gcagacgttc
      481 gcgcccagga ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat
      541 tttccgctgc cagcgggcgg caactggcgc attcaacaag ctgtaaccag cctgaatata
      601 gccaggctgt tgcagcagtt cgcgcatccc ttcgccgttg agctgccgtg cccaggcgaa
      661 atccgcaagg ttaaccgcgc cgcgttccag atgaacagat aacgcatcgg catcgctctg
      721 cgcccgtgca agagacgcca gccattgcag atattccggc ttacgcttac cgcgacgcgg
      781 cgggttaagc atcaccacac gcgcccccgc cagcgtgttg cgggccgaga tatcgcgcag
      841 cactatgcga tcgttatccg ccagccacag cggcgtgtcg aacaccagct cggcaaggtt
      901 atcttccagt agcgaaacac gtcccgtaac gtggctggcg gcgtggtgaa tatgcagcgg
      961 ctgccactgg gtcagcggtg tatgggtttg aagctcgaca atcacccgtg tgaacggctc
     1021 cggcggcaca tcggcaagca gccagtcgcc acggttaatc tgctcttttt ccgcatcacc
     1081 cgcgatgtta agcgcgatac gctgcccggc atgggcagtt tctgttggct gattttgcgc
     1141 atgcagcgca cgcacgcgca tcggtttatt tacaccagtc agccagagtg aatcgcctac
     1201 tttcacttcc ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa
     1261 tgcgcggtcg atcgcgaggc ggaaactatg ttggctggcg tgttcacgtt ctggtaactg
     1321 aagcagatgc tcgcgcaggg catcaattcc ccgaccttcg gttgctgcgg tgataaacag
     1381 ttttgcctca gcaaaaccgt attcccgcag cacctccttt acctggcgtt caacctcatc
     1441 aacacgcgct tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca tcgggttacc
     1501 ggtcagctgc aaaatcgcca gatgctcacg ggtctgtgcc atcacaccgt catcgcacgc
     1561 caccaccaac agcgcgtgat cgataccacc aacgcccgcc agcatgttgg aaagaaactt
     1621 ttcatgaccg ggtacgtcga taaaaccagg cacgcgacca tccggctgcg gccagtaggc
     1681 atagccgaga tcgatggtca tgccgcgctt tttttcttcc ggcagacggt cggcatttac
     1741 gcccgtaatc gcctgcaaca aggttgtttt gccgtggtca acgtgtccgg cagtcgtaat
     1801 aatcatttca acaacatctc caaaaaccgt tgctcatctt caaggcagcg taaatccagc
     1861 cacaatcgtc cgtcataaat acgaccaatc accggcactg gcaattcacg ccagcgggcg
     1921 gctaatgact caaggtggct accgcgtcca tcatgggggg taaacgttaa tgccgcgctc
     1981 ggcaggcgat caaccggcag cgaaccactg ccgatctgcg aaagacatgg cataacctgt
     2041 accgcaaact ccgcgccgta atgtgcggca aggagagcct gtaaacgttg tgcctggatt
     2101 tgaatgacct ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc actcagagct
     2161 tcagggtgta aataaagacg caacgtggct tccagcgccg cgagggtcat tttatccgcg
     2221 cgtaatgcac gcttcagcgg gtggctttgc aggcgggcga tcatttcttt tttaccaaca
     2281 ataattcctg cctgcggccc gcctaacagc ttgtcgccgg agaagctcac cagactgacg
     2341 cccgccgcaa tcaactcctg cggcattggc tctttcggca aaccgtactg gctaagatcg
     2401 accagcgagc cactgcctaa atcagtcacc actggaacat ccagttcttt gccgagcgcc
     2461 accagttccg cttcatctat cgctttggtg aacccctgaa tgctgtagtt actggtatgt
     2521 actttcatca acagtgcggt attttcattc accgcctgac gataatcatt cgcgtgcgtg
     2581 cggttggtgg tccctacttc gtgtagggtg catcctgcct gacgcataac atcgggaata
     2641 cgaaacgcgc cgccaatctc caccagttcg ccgcgagaaa ccaccacctc tttcccgctg
     2701 gcagtggccg ccaacatcaa taacaccgcc gccgcattgt tattaacgat acaggcatct
     2761 tccgcccccg taatacggca cagcagctgc gccagcgccc gatcgcgatg tccacgtccg
     2821 gcgtcgtcca gatcgtactc gagggtcact ggcgaacgca tagccttcgt aacggcttcc
     2881 accgcggctt ccgcctgtaa agctcgccca aggttggtat gcagcacggt tcccgtcagg
     2941 ttgatcaccg gacgcagcgc gctttgcgcc tctttcgtca accgggcatc gacttcttgc
     3001 gcccagtttt cacaccacgc gggcagcgtc tggctgtcac gaatcacttc tcgcgcttcg
     3061 tcaagcatct gacgcagcaa ttccaccacg cgggtgtgac cataagtatc acgcaaagaa
     3121 aggaaggagc tatcgcgcaa taagcgatca atagccggaa gttgactata gagggaacgc
     3181 gtttcggttg tcataggaaa cctggctgat caaggccctc tcacacggag aagggcgttt
     3241 aacataacca cggattgtaa cgtgagatgg gtcaggagga catatcgcgc atcaagcctt
     3301 tggcggttcg gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca gtttgactaa
     3361 gtgtgggcgg tcgacacacc agcctggcgc aacgcggcgg aaattgagat atccgaccgc
     3421 acaggcaatg gcgatagtcg ccagattgac cgtatcggtt ttgagcgtgc catcgaccag
     3481 atatccttcc agcacatcca gactgcggtt gattttctca cgctgacgta acaattcatc
     3541 ttcagactgc tgcgccgccg gacgcgcctg ttcacgcacc gataccagcc cggcatccat
     3601 aatgccatcc gccagcgcct caattttgcg cacccgcaac gactccagcg gatcacgcgg
     3661 caacatcgcc ggagcaacat tcattaattc aatatattca gcgatgatcg gcgaatcaaa
     3721 ccagcattcg ccctcttcgg tcaccagcac cggcactttt cctaacgggt taaattgcgc
     3781 cacgccgttg tccgcgttat agggcagttc attaataaat tcgaacgtta tgcccttttc
     3841 taacaacaga atagaaagtt tgcgtacaaa cgggctggtg tagctaccga cgagtttcat
     3901 gccgagtcct ttgtgcgagg aaaaatatca gtatggctca ggctcgcgca aaacacaggt
     3961 gatacaagtc tcaatgatcc aaataaagat aatgcatcca actggtcatt cttagcagca
     4021 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aactgccacc tgggcgtaga
     4081 tgccgtcggg taaggcatcg atatcagcgt tagggtccag ctcaatggtt cccagcacac
     4141 cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
     4201 ctggcacgac aggtaaaata ctggtcagtt tgccgtgaaa cacctgccca ggtagcgcgt
     4261 taaacaccac ttccgcatca tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt
     4321 gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg
     4381 cagcggcgta tgtacctggg cggatcagta cctgagtgac gtagccattg ctcggtgcgc
     4441 gaatgacggt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa
     4501 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
     4561 ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
     4621 cacgttccga gaacggattc accgccgcct ggctgccttt cagataacgt tgataatttt
     4681 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagtt
     4741 gcgcgcgcag cgtctttata ttatgcgtcg ccgtcatcag gtctgcctga agtctgtcaa
     4801 ctcgcgcctg gtaacgaacc ggctcgagct taaaaagcac ctcgcccttc tgaataagct
     4861 gattattttt gtcggtgact tcagtaacaa ttcccgtcac ctgtggcgtg atggggatcg
     4921 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaagatca
     4981 aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac tgatttaccg
     5041 gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa acaatcaata
     5101 gatccatatt tagatctccg gaaacgtgga atagtcagtc tttttctcgg cggcgagttg
     5161 atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag tcgctttctc
     5221 ttgcgacgca acatgtgact gcatgcccca gccgcgctcc ggttgataga gcgtcgccca
     5281 gatccacagg aacggccaga taacatgtag agtaaacagg ctcacccagc ccgccgtatg
     5341 aatagcgtcg gcatggggat ggttgcgctt tttggcaatc agataaggga tgtcatgtat
     5401 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
     5461 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg gctggcagcc
     5521 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
     5581 caaagaataa ttttaagagg tgatgagttg cttagttaca taacgatttc atgacgaagg
     5641 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
     5701 cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt taaatgccat
     5761 ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cattttgtga tgagcatcac
     5821 gtcaattacc tctctacccc ctacatttat gtgactgata tcacacaaaa ggccgccgac
     5881 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
     5941 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
     6001 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
     6061 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
     6121 ttccaacagg gtggttaccg aacgaaacgc tggcgaagct ggtcgggccg atgatcactt
     6181 atctcctgcc gctgctgatc ggttataccg gtggtaagct ggttggcggc gaacgtggcg
     6241 gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
     6301 tcggctctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
     6361 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
     6421 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt
     6481 ccaaaatgct ggcggcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
     6541 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
     6601 tctcgccgct gggtattcag cagtcccatg aactgggtaa atccatcttc ttcctgattg
     6661 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta
     6721 gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctggggggt atccacgaaa
     6781 tctacttccc gtatgtgctg atgaatccgc gtctgattct ggccgttatc ctcggcggta
     6841 tgactggcgt attcacgctg actatcctgg gcggtgggct ggtttctccg gcatctccgg
     6901 gttctatcct tgctgtactg gcgatgacgc caaaaggtgc ttacttcgct aacatcgcgg
     6961 gtgtgtgtgc ggcgatggca gtttccttcg ttgtctctgc cattctgctg aaaaccagca
     7021 aagtgaaaga agaagatgat attgaagctg ctgcacgtcg cgttcaggaa atgaaagctg
     7081 agtctaaagg tgcgtctccg ctgtctgcag gcgatgtgac taacgacctg agccacgtcc
     7141 gtaaaatcat cgttgcctgt gatgccggta tgggttccag tgcgatgggt gcaggcgtgc
     7201 tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac agcgcgatca
     7261 acaacctgcc gccagatgtg gacctggtca tcactcaccg tgacctgacc gaacgcgcta
     7321 tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcctgt
     7381 acaccagcct gaccgaacgt ctggttgctg cccagcgcca tactgaaaac gaagtgaaag
     7441 tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg
     7501 cggagaacat cttcctcggt cgcaaagcgg ccaccaaaga agaagcgatt cgttttgctg
     7561 gcgagcaact ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc
     7621 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
     7681 aagcgaaaga tcgcgtactc aaaacgggcg tcgtgttctg ccagtacccg gaaggcgtgc
     7741 gcttcggtga agaagaagat gacattgccc gtctggtgat tgggattgct gcccgtaaca
     7801 acgagcacat tcaggtgatc accagcctga ccaatgcact ggatgatgag tccgtcatcg
     7861 agcgtctggc acacaccacc agcgtggatg aagtgctgga attgctggca ggtcgtaagt
     7921 aatccattcc caccctctcc gcatgaagaa ggtggggcta attgcttgat gcgcttcgct
     7981 tatcaggcct acaacataaa gcacaatttg ttttgtcggt cggataaggt gtttacgccg
     8041 catccggcag tcggtgcaca atgcctgatg cgacgctgac gcgtcttatc agacctacag
     8101 gtcagtgcca aacacgtagg ccggataagg cggtttacgc cgcatccggc aaaaagcccc
     8161 tctcctcacg gagagggttt gggtgaggga aaagcctcac cccagccctc tcgggtaaaa
     8221 acattgatga aggttaatac tatgaaagca ttacattttg gcgcaggtaa tatcggtcgt
     8281 ggctttatcg gtaaactgct ggcagacgca ggtatccaac tgacgtttgc cgatgtaaat
     8341 caggtggtgc ttgatgccct gaatgcccgt catagctatc aggttcatgt ggttggtgaa
     8401 accgagcagg tagataccgt ttctggcgtc gatgccgtca gcagcattgg tgatgatgtc
     8461 gttgatctga ttgctcaggt tgatttagtc actaccgccg ttggcccggt cgtgctggaa
     8521 cgtattgctc cggccatcgc caaagggctg gtgaaacgta aagaacaagg taatgaatct
     8581 ccgctgaaca tcatcgcctg tgaaaacatg gtccgcggca ccacgcagct gaaaggccat
     8641 gtgatgaacg ccctgcccga agacgccaaa gcgtgggtag aagaacacgt tggctttgtt
     8701 gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg caactaacga tccgctggaa
     8761 gtgacggtag aaaccttcag cgaatggatt gtcgataaaa cccagttcaa aggcacgctg
     8821 ccaaacatcc ctggcatgga attaaccgac aatctgatgg catttgtcga acgtaaactc
     8881 ttcaccctga atacaggtca tgctataacc gcgtacctcg gaaaactggc gggtcatcag
     8941 accattcgtg acgcaattct cgacgagaaa atccgcgcgg tggtaaaagg ggcaatggaa
     9001 gaaagtggtg cagtattgat caagcgctac ggctttgacg ctgacaagca tgcggcatac
     9061 atccagaaaa tccttggtcg tttcgagaac ccgtacctga aagatgatgt agaacgcgta
     9121 ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc tgatcaagcc actgctcgga
     9181 accctggaat atggtctgcc acataaaaac ctgattgaag gtattgccgc tgcaatgcac
     9241 ttccgcagtg aagatgatcc gcaggctcag gaactggcag cgctgattgc cgacaaaggc
     9301 ccacaagccg cgctggcaca gatttccggt cttgatgcca acagcgaggt tgtatccgag
     9361 gcggtaaccg cttataaagc aatgcaataa tggtggacca ggcgcaggat accctgcgcc
     9421 cgaataacag attgtcagat atgcaggcaa caatggaaca aacccaggcc tttgaaaacc
     9481 gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc accgccgtcg
     9541 agcttttgac cgaggcggta aatcttctgg tgcttcaggt attccgcaaa gacgattacg
     9601 cggtgaagta tgctgtagaa ccgttactcg acggcgatgg tccgctgggc gatctttctg
     9661 tgcgtttaaa actcatttac gggttgggcg tcattaaccg ccaggaatac gaagatgcgg
     9721 aactgctgat ggcattgcgt gaagagctaa atcacgacgg caacgagtac gcttttaccg
     9781 acgacgaaat ccttggaccc tttggtgaac tgcattgcgt ggcggcgtta ccaccgccgc
     9841 cacagtttga acctgcagac tccagtttgt atgcaatgca aattcagcgt taccaacagg
     9901 ctgtacgatc aacaatggtc ctttcactga cagagctgat ttccaaaatc agcttaaaaa
     9961 aagcctttca aaagtaagta ctttcgctta cttctcctct acctgtttcg gacgataaag
    10021 ctgacgataa tactccagac gttgtagata taacggcacg ctttcatcag gtattccgga
    10081 cggaatcgcg ttacggggag gtagtttttt cagatactcc cggaacgcct ggctcgatgc
    10141 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
    10201 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttagatcccg
    10261 gtataaatga cattaaggaa aacgtgctca atcctcaata
//
LOCUS       DACADA010000154        10180 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.156, whole genome shotgun sequence.
ACCESSION   DACADA010000154 DACADA010000000
VERSION     DACADA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.156"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..1626)
                     /locus_tag="ISB17_003874"
     CDS             complement(<1..1626)
                     /locus_tag="ISB17_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1981756.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQ"
     gene            complement(1572..1793)
                     /locus_tag="ISB17_003875"
     CDS             complement(1572..1793)
                     /locus_tag="ISB17_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981757.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1838..3775)
                     /locus_tag="ISB17_003876"
     CDS             complement(1838..3775)
                     /locus_tag="ISB17_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP1981758.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(3839..5500)
                     /locus_tag="ISB17_003877"
     CDS             complement(3839..5500)
                     /locus_tag="ISB17_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP1981759.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTNLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5497..6060)
                     /locus_tag="ISB17_003878"
     CDS             complement(5497..6060)
                     /locus_tag="ISB17_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP1981760.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNLEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6351..6716)
                     /locus_tag="ISB17_003879"
     CDS             complement(6351..6716)
                     /locus_tag="ISB17_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP1981761.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCAECHRQKSAREAASGMPD
                     PTLPEVSGGSGREDDIIGL"
     gene            6758..6958
                     /locus_tag="ISB17_003880"
     CDS             6758..6958
                     /locus_tag="ISB17_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAP1981762.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7157..7372)
                     /locus_tag="ISB17_003881"
     CDS             complement(7157..7372)
                     /locus_tag="ISB17_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981763.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVLQPVC"
     gene            complement(7458..7643)
                     /locus_tag="ISB17_003882"
     CDS             complement(7458..7643)
                     /locus_tag="ISB17_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981764.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(7865..7951)
                     /locus_tag="ISB17_003883"
     CDS             complement(7865..7951)
                     /locus_tag="ISB17_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981765.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            complement(8506..9039)
                     /locus_tag="ISB17_003884"
     CDS             complement(8506..9039)
                     /locus_tag="ISB17_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP1981766.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VFRRDQESALACWGIDR"
     gene            9145..9417
                     /locus_tag="ISB17_003885"
     CDS             9145..9417
                     /locus_tag="ISB17_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981767.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            complement(9383..9727)
                     /locus_tag="ISB17_003886"
     CDS             complement(9383..9727)
                     /locus_tag="ISB17_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAP1981768.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKSPPKW"
     gene            complement(9732..9947)
                     /locus_tag="ISB17_003887"
     CDS             complement(9732..9947)
                     /locus_tag="ISB17_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1981769.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT         2279 a         3135 c         2429 g         2337 t
ORIGIN      
        1 ctgcagtaca tccggatcat aatcacgacc atcacgcggt accggaatgg cccccacagc
       61 gtcatccacc atcgcctgca gaaccggacg cacctcatcc accgtcacat gcttctgtaa
      121 taccgccgac agggaagtca gtttctcttc aaacgcttgt gcctgcgagg ccatcttccc
      181 ctcaaatgtg cgctgtaaat ccgccagcac tgtggagaat tcttctccca gtgcacgaat
      241 aatggacagt tcccgttccg tcattttctc agtatccccc ctgaacatcg ctttcaccgc
      301 atcatgctct gtttcactga ttgccttatt accgtcagat gcgccgtcag gcagctgtga
      361 tgaaactgtt ttcccggcag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc
      421 ctccagactg tagttctgct gctgaagata cagtgcatca ccgccggcca ggggcggcag
      481 gttctcccgt ttacgggcct cattgggcgt gagaagcgta tttttcaccg catcccccag
      541 cgttttcatg cgccgctcac tgtccattct cagcagcgtg gtgacatcaa attctgtact
      601 ctcgttttcc cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt
      661 ctgcaggcac tgggaataat actgctgctc cagcgcctcc acgttgtcac tggaaggcgg
      721 ttgtcccacg ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagcggt
      781 catcttcagt tgttccaccg tctgcgcatc cacaggtgaa aacgtcgtgg ggttgtattt
      841 tgccccgttg ctcagaatgg ccgttttccc cgcattttcg cctgtatacc cgctgtccca
      901 gttgctcttc agttttttcg cattttcttc cgtaatactg ccggggatct caatcacccc
      961 ggacggcctg ccgccatttc tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg
     1021 cgtggccgcc agcccggcgg catacaccgg cggcaatcct ataagcggat gaaaaaaaca
     1081 gttaaaccgg tcgtggatca cttcccgggc aggcaccgtc accgcctccg tgatcccgca
     1141 gttccggtcc ggcgtgatgc ggtagaacac ctcgccgtca tccgccacca gaggttcaac
     1201 ccggctccag tccagaatac gcagttcttt gatctgcccc cgggcattac ggattttcag
     1261 caccaccgta ttgccatgac gcagtttggc gttcagccac agttcaaaaa actggatgcg
     1321 gttctgctgg gcgttgggac gacgacagag gcgggcaata tccccccggc gcgtttccct
     1381 gcgtatccca tgcgcatccg tctgcataag acgcagccgc attttggcga tatcctggga
     1441 tatcagcgaa atacatgcaa acaccgcatg aaaggagagg acggcttcag gatcggcttt
     1501 cacgccctgc tgccaggcgc cggaaaaggg ctcagccacc gcctgaaaca ggctggtcca
     1561 gcccgcctct cttacgtcac gtcctgattt ctggtttttt cgggttcgcc gtaaaaggtt
     1621 ccacattcgc catgctccgc atcacgtttc tttttctgac ctgccggacg tcgcaccgtg
     1681 atgtactccg cctttcccag gcgaaccagc acctccgcac acggctgtgc cacatcacgg
     1741 atatccccgg cccgggcatc atgcgtgccc tgcagatatc ggatctttgc cataacctgt
     1801 tacgggaggc gcacgcctcc cgtcctcctt atcagactca gccgccggac gcactgccgt
     1861 agttcactcc ggtgatcacc gccaccgccg cagtacggcg acgacgccag ttgatccagc
     1921 gctccgcacg gatggccacg ctgcctgtct ggaacatgga aaccagctcc accggggacg
     1981 gcgtggtact gtcgccggtc ggctcagact gcatttccag tgatgcctcg cgggacatat
     2041 ccactgccac gccgccgtca tccgccagat aaatatccgg ggcattcacc agcaccagct
     2101 ggtcacccac gtactgggag acaatcaccg gcagcccctg gaaggagcca cccagcaggg
     2161 tcatgtccgg gtattccttc tgacccagcg catttttacg catggacagt gccagggcat
     2221 tggtgctgga catcagccag accgcaccgg tgggctgcag gtttgctgcc acaaactgtc
     2281 caaacgcagc ctctgcatcc gcatccgggt taccggttga tgccgtgccc ttcacatcat
     2341 gggtgatgga cgccggggag acatctgcca ctgcggcttt tttcgggtcc acaaagtctg
     2401 tatccagacg cgccaccacc gcttccgcca gcgcattacg gaccagtgca tcagcagccg
     2461 gactggaaaa acggatcaat tcttccgtca gtaccgcaat ggccgacacc ttcgcatgac
     2521 tgaaggtgat ggattcaaaa tcaaacttcg tcaggggttt tgccttaccc tcacccaccc
     2581 agccggcagc accgccggac acctgggcgt gcacacggat attgaatggc acctgacgaa
     2641 gtgcagggat cccgccctga ccaaatcgcc cgataatggt ctgcggacgc aggtaatcaa
     2701 taaagtcctg tgcgtattcc tgatattcag acaggctgcc tgcccactgc ggatccgtgg
     2761 tggtccccgc gcccactgcc gatttcagga catgatgcag acgactgtca tccggatact
     2821 gacgacgggc cacttccagg gcttcagatc ggacgccttt agccgcagcc agcgatttgg
     2881 caaagcgggc gaagccaatc cccttatcca gtttctgctc cacacggatc accggcgcag
     2941 aagccaccgc ggccacattc ccgttaccgg cctgtttcac cggctgcgcc gtggcggcct
     3001 taccggcttc cagttcacgc aggcgcttca ggtgcgcatc cacctgacgg atttccgctg
     3061 cggtgttgtc gtaatgctct tcctcctcca catccagcgt gcgcccttcc tctgcggctt
     3121 tggtcatgac ctcctcaagg gaggctgcca gcgctgcacg cttgttttca aaacttttaa
     3181 tctgttcgcc aatattcatt atggtctttt ccttatgaaa aacggttgtt gactgtgccg
     3241 cagcgccggc agaagatgcg attttcacca ccggtttccg gttgccggac gcggcagaaa
     3301 acgggcggtc gtaagattta atggtccgga tggtgcattc cgcattcgcg ggcacggtga
     3361 cggcagacac ctccatcagt tcccagcgca gaaaatgcag tccgcctccg tccagaaagg
     3421 tgtattcatg gggacggaag cccacggaca gccccctgac cagcccggtc ttaatggccg
     3481 cccagacctc atccagccgg gcagccagtt gcgacggcat atccggtacg ggcttcacca
     3541 gtgttgccgt gatttccagc ccttcgctga cccggcgcac cgtacactgc cccaccgggc
     3601 gggaatggtc atgctgccag agaaacggga tcgcactgcc aaactccgcg ccctccggct
     3661 ccaggatgtc accatcccga tccggagaag gcgttgacgc aatcccggtg atcacccgtt
     3721 catcctcact gaaggatttc accgtcagca gggaacaggc ccgtttaaga gtcacatcag
     3781 cctcctgaaa ataaaaaaac cgccggagcg gttcgtgatg gttacagtgt gaacagggtt
     3841 atatgaaaaa aaccgcatat tctttctttt tcggttccgg gttaagggac atcagggaga
     3901 ccgcattgaa cagcgccatc agcgggtcaa tttttccccg tccactggcc tgtttggtga
     3961 taagaatggc gttaccttta ggctccaccc gggcattgcc gacacaccag gccatcaggg
     4021 gctggtcacc atgcaccagc accccttcag ccagtttgcg ctcggtggtt ttaatggccc
     4081 cgcccagttt ccagccctgg cttatcccca ccacaattcc gtcggggatc ccggcttccg
     4141 ccagtgaatc cagaatctgc cccacccctg acgggtcaat accgatatgg tccagtaact
     4201 cagcctcatg aatgcgacgc acatattccg ccacttccgc cgtgtcatcc ccgacacgcc
     4261 ggacaatggt catatctcca caggcaacaa gatcctgaaa ccgggacgcc tcgctcttcc
     4321 gtcggaccac cgcggtttca tgcgcccagg catggcccca gcccagccat tcgcgggtct
     4381 cccggtcacg cccaatcaca tacatcccca gcagatcatc cagccctccg ccgtcaatcc
     4441 ccaccgtcac cacatcagca cgacgcagga tatcgtccag gctgatacaa cggccctgct
     4501 cttcccagaa atcagccccc gcccagcggt cagagcgcag ggcaagacca atttccacat
     4561 tggcgtgttt tgacatgaac ccccggaatg tctcttcacc ggcttcccgg gctttacggt
     4621 actcccggta cagaaaggcc tcatccactg aatagccgag attcggattg accatggcga
     4681 ggttttccat cagcaggtta gtcccgcttt ccaccatttc aggagggtgt tcaaatatca
     4741 ccggcagaaa gtgcggatca tgaattttgc cgtcgcgcac atcccgggcg tactgcagtt
     4801 tctgtctgaa caccccggcg ggcggttcat tcgactgggt ggtcgtatac accacaaacc
     4861 cttccgggcg ggaggcaagg ccgcctatgg cttcacgtaa catgtcctcc gccttgcact
     4921 gcttgccaaa cagccacaac tcatcaatca gcgtacccac ggacttgata ccggacaccg
     4981 tattcggatc ggctgccacc accttcaggg tggtgtccgt cactcggtgg gtgatggtcc
     5041 ggatatgggt ctgtacctgg cagaggtcat ccagatcatc gtcacgtcgt accatatccc
     5101 gggcagggtt gaaggcgttg gccgccacct ccacagtcgg ggccagaatc gtgtaacccg
     5161 ccgcctgccg ccagttcagt aacagtgcag tcatcatgat ccccgcggcc agcgtggact
     5221 tcgagttttt cttggggata aggataaaaa cttccttgat atggcgtaca ccggtctgcg
     5281 catcgtagga gccaaacagg gccgccacca ggtcaaacac ccacggtgca caggactccc
     5341 cgaacgtagg gctaccaggt gcatccacaa tccgcagttg tttaaaaatc gccagggcat
     5401 gtgcggcctc gtccggataa atcggatccg gaataatcga cagccccttt ttcaggcgct
     5461 ctgcccagtc cgggcaggct gtgctccata caggtatcat ccgttgccct cattatcgtt
     5521 attcaccacc agtcgggggg gcggtggcac cgcaaaacgg ttagccgctt ttttcgcggc
     5581 atcacctttt gccgattttt tcccggtatc ccctttttta tggtgcgtga actgcgccag
     5641 acgccaggcc gcatccagtg ccagtttcgg atcaatgcag aggttttcca ccaggatccg
     5701 ccccatggct ttcaccggat cgggaagacc atcctccata tattcaatac caggagacat
     5761 caccgcggac ggtggcatct ccagattgtt ttcgtccggc tgtggtattg cagccgcctc
     5821 acggcgacgg ggtttatcct cctgctctga ttttttctgc cggtaaacag gaacctcatc
     5881 cacctccacc gtctcgcatt gtttacgggc tataaacgca agcacctcag gatcttttgc
     5941 cagctgcgag cctttaaccc tggcggtctt cgccgaataa ccggcggcaa tggctgacgc
     6001 tgttttgttt ttcccggaca tgagcgccag cgcaaatttt cgtttttgcg ttgtcagcac
     6061 agcctcctcc cgggtccaga acgcactcag ccgggtatgg ttcagcccat ttttcccggc
     6121 gtctcatgcc gcaaatgtta actgctgcct ggttaacatt tgctgaaaaa gcctgttaac
     6181 attttttccg cacaacaaac tgaataataa agataaaaac cgcaaaaatg cccgggcagc
     6241 cagttaacat gttaactgcc ctgaaacggg aattttttct ctgcgtgaga gggggcgcgg
     6301 tgtccaaagc gatcgttttt tacgccggat gatacccccc ccgggttggg ttacagtccg
     6361 atgatgtcgt cctctctgcc actacctccg gacacctccg gcagcgtcgg gtccggcata
     6421 ccactcgccg cttcacgagc agacttttgt cgatggcatt cggcacagag cgtccaaaga
     6481 ttcgtctcct cattaccacc accgaactga agtgcaattc ggtgatcgag ttcactgtca
     6541 cagaggtcaa ccacacgacc acagatacag cactgcccgg cgtccctcag ccagatatga
     6601 cgcttgaggg aaacacgtgc actgccactg acacgacgct gctccccctt cagaatattc
     6661 acccgtcggg tattcagtgt tttgattctg ctctggagtg tacgaagctc agccatgtaa
     6721 aatccccgtc atatggcaat cagtaaagga aataaatatg tcatcgaaaa accggacccg
     6781 cagaaccaca acccgcaata tccgtttccc caatcacata attgaacaga tcaacatcgc
     6841 ccttgagcat aaagggtccg gtaacttttc agcgtgggtt attgaagcct gcaggagaag
     6901 gctggcaaca gatgcaacgc atctgcgccc ggccagcatg acaaataacg agaaatgaac
     6961 gttcggtttc ttccaccatc gcaccggaca ggcgactatg aggggacaac gccgcgctcc
     7021 gttaacgcgg taaaccccgg tgtgtatcgt ttttgattat ccccgcacac tcgcgcagag
     7081 gagtctccct gtcgggctgc ggtctctgtt aatgcaggaa tacggcgaca ataccgcgca
     7141 tggataataa ggtcgctcaa cacactggct gtaataccat gcggcattta gcggcattca
     7201 tcgtacactc cacggttagc tcttcattcg tggcattcac ctgaaaggtc cgggagtgta
     7261 attgcgtaca tttaccactg aacgaacctt caacaagaac acgaccacgc tgcaaaatac
     7321 ggaacggaat tgttccctga aaaggttcta cggttacccg taatttcttc atgtatcctc
     7381 cggataataa aaagccagct tagtgcactg agtgcggata tattcctgcg ccccttccag
     7441 ctgcttctgc attgtcatca accgttctct gaggatgaaa taatcccgtt cagcggtgtc
     7501 tgccagtcgg gggccggttg cattatccac gctggaggtg ccggtggctt cacgcacggt
     7561 accggggcag gtggcgttga cccgcaggcg cttacgacca gcggcaacat cagcgcgcag
     7621 agtttcattt tcagctctcg catcggctaa ttccttcgtg tatctggcat caagcgcagc
     7681 gacatctcgc tggcgctgct gcatgtcagt aatagtggca ttcgccagtt tcagctcact
     7741 gactttttta tcgcgctgcg ctttgtaggt gatggcgtta tcgcggtaat gattcagccc
     7801 cagactaagc acaccacagg ctaccagcat gacaataatc accacacaca gaacacggtt
     7861 catatcacca ccaacggatt gcccagacca gaacagcaat ggccacaata cgaatggcaa
     7921 atgccattgc ccgaataagt tcagcactca tctttttaaa gttcacgatt tcagcgcaat
     7981 gaccagtttt gccagcccat acagcatcgg agacacagca ataccaacag ccacccactt
     8041 aatagcaaaa gccagcgctc tgctgatgtc atcagtcact gtcaccccag cagccccgac
     8101 gaagacaaca tcacccaggc gagggacaga aaaagagcaa ccagcattag tgaaaatgaa
     8161 ataccgacaa tcacacacag gacctttgcc ggcgttatga gtttgtctga catagctacc
     8221 ccttaattgc cacaattaac tgggatacta cccataaaaa agggatgctc cagaccagca
     8281 aaaatttcca gtttggtaat tgactaatca tgagtcgcaa ctccctaatc agtttgctaa
     8341 aatcaatcaa ggcagcctcc catagcttac tgccataaaa acaaaacccc gcttgctgcc
     8401 aacaaacggg gtttttactt ttattcactt aggttttgcc agttcacagg acttcgtgtt
     8461 atccgcccgc gttggccaac ctcatttttc agcaaaatat tctgcttatc tgtcgattcc
     8521 ccagcacgcc agcgcgctct cctggtcacg acggaatacc tgaccgtaac agttatttga
     8581 gcgaatacgg cagtctctgc caccgtcctt aatccaccag cgaatcgctt cgcatgctcc
     8641 cctgcgatca cctgcattaa ttcgtctgta aaacgtcgac gggaaacact taccgggacc
     8701 aatgttgtac ggacagaatg acgcgatccc cgctttctgg ggttcggtca atggcacttt
     8761 gatgtttttc tccacccatg ccagcgcctt atcacgctca atggcgttaa cccggtcgca
     8821 tttttccttc gacaacttca tgcccggaac gacaggtttg ccatccacca ggatggcacc
     8881 gcggcagatg gtccagatac ccgcgccatc acggtatgcc gtggtgtggt taccttcctt
     8941 ttcatccaga aactggtcga gaatgtcagg cgcaggcgca ccagcggcaa tcagcgccag
     9001 aacggcagcc gacaggccgt atctgatttt tgcgttcatg gatatttatc aggatttatc
     9061 ggtttctgag ccctggatat gtttatcagt tccagcctgt tgcctcaggc tgctaacagg
     9121 tcaatacaat catgaggatt atttatggac aataacacca tttctctaca ggagttgctc
     9181 gacagcattt ccaggcttcg ggaagacgtg aataccctta ccgtcgcctt ctcatatctg
     9241 gcattctcaa ttccaaggga acagatgcaa tcaacgctgg catcaatcca gtttgaatca
     9301 tgcaatccca aatggtctca ggaacaacaa gactctttca ggcggcttgc tgtattactg
     9361 gatgaaaaat atgctggtaa aattaccatt tcggcggact cttcagagaa cccgtaatta
     9421 ttcccggtag ttttcctctg taggttatca acacatcctg cgcctctaaa attacggggc
     9481 gcttttccgg cgactgctca tccccttcac ataacccggc agcaacatcc aggaagacct
     9541 gtctgatgct ccttctggct gctgcctcat aaaactccag cgcggcacct tcaacacggt
     9601 ccagcgagat gtccaggtca aaaatttcac cgtcaaagcg ttttttgtcc cgtaacgcta
     9661 aagttaccgt aactttattc tcaaaattgc ggatcccttt cacaatcagt tcatagtttt
     9721 gagtcattga attactctcc ccgtgccgcc ttacgacggt cctctctgat tttgaaatac
     9781 aggttagtca gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc
     9841 cactgagacg gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct
     9901 gacgtggtgt atgacacacc tgttgtgatt ttttccatct ggtacatacc ccgtctcccg
     9961 ttatccggaa gctgacaaca ataaaaaaag ccaccagtta agtactgatg gctctgataa
    10021 ctcatgcagg catctcagac gacccactga cactaccggt gagtttaacg ataccttcca
    10081 tttgactggc tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct
    10141 atctcaacgc catacgttgc atttttggta atatccgtca
//
LOCUS       DACADA010000155        10148 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.157, whole genome shotgun sequence.
ACCESSION   DACADA010000155 DACADA010000000
VERSION     DACADA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.157"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            70..1464
                     /locus_tag="ISB17_003888"
     CDS             70..1464
                     /locus_tag="ISB17_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP1981770.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1541..2038
                     /locus_tag="ISB17_003889"
     CDS             1541..2038
                     /locus_tag="ISB17_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAP1981771.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLIAK"
     gene            2133..2840
                     /gene="endA"
                     /locus_tag="ISB17_003890"
     CDS             2133..2840
                     /gene="endA"
                     /locus_tag="ISB17_003890"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAP1981772.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2920..3651
                     /gene="rsmE"
                     /locus_tag="ISB17_003891"
     CDS             2920..3651
                     /gene="rsmE"
                     /locus_tag="ISB17_003891"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP1981773.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3664..4614
                     /gene="gshB"
                     /locus_tag="ISB17_003892"
     CDS             3664..4614
                     /gene="gshB"
                     /locus_tag="ISB17_003892"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAP1981774.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQVGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4651..5286
                     /locus_tag="ISB17_003893"
     CDS             4651..5286
                     /locus_tag="ISB17_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAP1981775.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5286..5702
                     /gene="ruvX"
                     /locus_tag="ISB17_003894"
     CDS             5286..5702
                     /gene="ruvX"
                     /locus_tag="ISB17_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP1981776.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5817..6797)
                     /locus_tag="ISB17_003895"
     CDS             complement(5817..6797)
                     /locus_tag="ISB17_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAP1981777.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6815..7519
                     /gene="yggS"
                     /locus_tag="ISB17_003896"
     CDS             6815..7519
                     /gene="yggS"
                     /locus_tag="ISB17_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAP1981778.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7537..8103
                     /gene="yggT"
                     /locus_tag="ISB17_003897"
     CDS             7537..8103
                     /gene="yggT"
                     /locus_tag="ISB17_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAP1981779.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8100..8390
                     /locus_tag="ISB17_003898"
     CDS             8100..8390
                     /locus_tag="ISB17_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAP1981780.1"
                     /translation="MSAVTVNDGGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8398..8991
                     /locus_tag="ISB17_003899"
     CDS             8398..8991
                     /locus_tag="ISB17_003899"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAP1981781.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            8984..10120
                     /gene="hemW"
                     /locus_tag="ISB17_003900"
     CDS             8984..10120
                     /gene="hemW"
                     /locus_tag="ISB17_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAP1981782.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2379 a         2684 c         2688 g         2397 t
ORIGIN      
        1 ttcacatctt tttacgtcgt actcacctat cttaattcac aataaaaaat aaccatattg
       61 gagggcatca tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat gacgtttttc
      121 gtctgcttcc ttgctgctct ggcgggatta ctctttggcc tggatatcgg tgtaattgct
      181 ggcgcactgc cgtttattgc agatgaattc cagattactt cgcacacgca agaatgggtc
      241 gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg gcagcggctg gctctccttt
      301 aaactcgggc gcaaaaagag cctgatgatc ggcgcaatcc tgtttgttgc cggttcactg
      361 ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt cccgcgttct gctggggctg
      421 gcggtgggtg tggcttctta taccgcacca ctgtacctgt cagaaattgc gccggaaaaa
      481 attcgcggca gtatgatctc gatgtatcag ttgatgatca ctattgggat cctcggtgct
      541 tatctttctg ataccgcctt cagctacacc ggtgcatggc gctggatgct gggtgtgatt
      601 atcatcccgg caattttgct gctgattggt gtcttcttcc tgccagacag cccacgttgg
      661 tttgctgcca aacgccgttt tgttgatgcc gaacgcgtgc tgctgcgcct gcgtgacacc
      721 agcgcggaag cgaaacgtga actggatgaa atccgtgaaa gtttgcaggt taaacagagt
      781 ggctgggcgc tgtttaaaga gaacagcaac ttccgccgcg cggtgttcct tggcgtactg
      841 ttgcaggtaa tgcagcaatt caccgggatg aacgtcatca tgtattacgc gccgaaaatc
      901 ttcgaactgg cgggttatac caacaccacc gagcaaatgt gggggacggt gattgtcggc
      961 ctgaccaacg tacttgccac ctttatcgca atcggccttg ttgaccgctg gggacgtaaa
     1021 ccaacgctaa cgctgggctt cctggtgatg gctgctggca tgggcgtact cggtacaatg
     1081 atgcatatcg gtatccactc tccgtcggcg cagtatttcg ccatcgccat gctgctgatg
     1141 tttattgttg gctttgccat gagtgccggt ccgctgattt gggtactgtg ctccgaaatt
     1201 cagccgctga aaggccgcga ttttggcatc acctgctcca ccgccaccaa ctggattgcc
     1261 aacatgatcg ttggcgcaac cttcctgacc atgctcaaca cgctgggtaa cgccaacacc
     1321 ttctgggtgt atgcggctct gaacgtactg tttatcctgc tgacattgtg gctggtaccg
     1381 gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg
     1441 cgcgaaatcg gcgctcacga ttaatctcct caggcttcct cccgtcgcgg aggaagccac
     1501 ctcttgcagt catcttttct tcgctctatc ctctgccgct atgaaaacat cccgtctccc
     1561 tatcgccatc caacaggccg ttatgcgtcg cctgcgggaa aaactcgccc aggccaacct
     1621 gaagctaggg cgtaactacc cggagccaaa actctcttac acccagcgcg gaacctccgc
     1681 cggaacagcc tggctggaaa gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa
     1741 cagtgaagcc tttattgaag aagtggtgcc gcacgaactg gcacatttgc tggtatggaa
     1801 acatttcggc cgcgtagcgc cacatggcaa agagtggaag tggatgatgg aaagcgtgct
     1861 gggcgttccc gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt
     1921 cccctaccgc tgcaagtgcc aggagcatca gcttaccgta cgccgccata atcgcgtagt
     1981 tcgtggcgag gccgtctatc gctgtgttca ctgcggtgaa cagctgattg caaagtaacc
     2041 acctgaacta tcaggaactt tcctgatctg gctgattgca taccaaaaca actttcgcta
     2101 cgttgctggc tcgttttaac acggagtaag tgatgtaccg ttatttgtct attgctgcgg
     2161 tggtactgag cgcagcattt tccggcccgg cgttggccga aggtatcaat agtttttctc
     2221 aggcgaaagc cgcggcggta aaagtccacg ctgacgcgcc cggtacgttt tattgcggat
     2281 gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg
     2341 tgcgcaaaaa tgaaaaccgc gccagccgcg tagagtggga acacgtcgtt cccgcctggc
     2401 aattcggtca ccagcgccag tgctggcagg acggtggacg taaaaactgc gctaaagatc
     2461 cggtctatcg caagatggaa agcgatatgc ataacctgca gccgtcagtc ggcgaggtga
     2521 atggcgatcg cggcaacttt atgtacagcc agtggaatgg cggcgaaggc cagtacggtc
     2581 aatgcgccat gaaggtcgat ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg
     2641 gtgccattgc gcgcacctac ttctatatgc gtgaccaata caacctgaca ctctctcgcc
     2701 agcaaacgca gctgttcaac gcatggaaca agatgtatcc ggttaccgac tgggagtgcg
     2761 agcgcgatga acgcatcgcg aaggtgcagg gcaatcataa cccgtatgtg caacgcgctt
     2821 gccaggcgcg aaagagctaa cctacactag cgggattctt tttgttaacc cctaccccac
     2881 gcgaacaacc gcgtggggag acgacgcgga tttttaacta tgcgtatccc ccgcatttat
     2941 catcctgaac cactgaccag ccattctcat atcgcgcttt gcgaagatgc tgccaaccat
     3001 atcgggcgcg tactgcgcat ggggccgggg caggcgttgc aattgtttga cggcagcaac
     3061 caggtctttg acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgttg
     3121 gaaggacaga tcgacgatcg cgaatctccg ctgcatattc acctcggtca ggtgatgtcg
     3181 cgtggtgaaa aaatggaatt tactatccag aaatcgatcg aactcggtgt aagcctcatt
     3241 acgccacttt tttctgagcg ctgcggcgtt aaactggata gtgaacgtct gaacaagaag
     3301 ctccagcagt ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg
     3361 ccggaaatcc gtccagcgat ggatctggaa gcctggtgtg cagagcagga tgaaggactg
     3421 aaactgaatc ttcacccgcg cgccagtaac agcatcaata cgttgccgtt accggttgaa
     3481 cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg cagatgaaat tgccatgact
     3541 gcccgctatc aatttactga tatcctgttg ggacctcgcg ttttgcgtac agagacaact
     3601 gcgctcaccg ccattaccgc gctacaagta cgatttggcg atttgggcta acggagaaga
     3661 ataatgatca agctcggcat cgtgatggac cccatcgcaa acatcaacat caagaaagat
     3721 tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt acgaacttca ctatatggag
     3781 atgggcgatc tgtatctgat caatggcgaa gcccgcgccc atacccgcac gctgaacgtg
     3841 aagcagaact acgaagagtg gttttcgttc gtcggtgaac aggatctgcc gctggccgat
     3901 ctcgacgtga tcctgatgcg taaagacccg ccgtttgata ccgagtttat ctacgcgacc
     3961 tatattctgg aacgtgccga agagaaaggg acgctgatcg ttaacaagcc gcagagcctg
     4021 cgcgactgta acgagaaact gtttactgcc tggttctctg acttaacgcc agaaacgctg
     4081 gttacgcgca ataaagcgca gctaaaagcg ttctgggaga aacacagcga catcattctt
     4141 aagccactgg acggtatggg cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac
     4201 ctcggcgtga ttgccgaaac cctgactgag catggcactc gctactgcat ggcgcaaaat
     4261 tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg tggtagatgg cgagccggta
     4321 ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt
     4381 ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga aaatcgcccg tcaggtcggg
     4441 ccgacgctga aagaaaaagg gctgattttt gttggtctgg atatcatcgg cgaccgtctg
     4501 actgaaatta acgtcaccag cccaacctgt attcgtgaga ttgaagcaga gtttccggtg
     4561 tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt tacagcagca gtaacccacc
     4621 ttagcgagaa ggatctcgtt gagactctga gtgacagcgc ccttctttcc acgcatactg
     4681 ggcgctgttg cttttttgaa ccaggaaaca gaacctctga caatgaattt acagcatcac
     4741 tttcttattg ccatgcctgc tctccaggat ccgattttcc gtcgttccgt ggtctacatt
     4801 tgcgaacata ataccaatgg tgcaatgggg atcatcgtca acaagccgct ggaaaatctc
     4861 aaaattgaag ggattctgga aaagctgaag atcacgccgg agccgcgtga tgaatcaatc
     4921 cgtctggata aaccggttat gctcggcggc cctctggctg aagatcgcgg gtttattttg
     4981 catactccgc cctccaattt tgcttccagc attcgcattt cagacaacac ggtgatgacc
     5041 acttcccgcg atgtgctgga aacgctcggc accgataaac aaccgtctga cgtattggtg
     5101 gctctgggct atgcctcctg ggagaaaggt caactggaac aagaaattct cgataacgcg
     5161 tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa caccgattgc cgaccgctgg
     5221 cgcgaggcgg caaaactgat tggtgtggat attctcacca tgcctggtgt ggcagggcac
     5281 gcctgatgag tggaacctta ctcgccttcg acttcggtac caaaagcatt ggtgtagcgg
     5341 tcggccaacg cattaccggc accgctcgcc cattgcctgc aattaaagca caggacggta
     5401 cgccggactg gaacctcatc gaacgtttac tgaaagagtg gcagccggac gaaatcatcg
     5461 tcggtttgcc gctgaatatg gacggcaccg agcaaccatt gactgccaga gcgcgtaaat
     5521 ttgccaaccg tattcatggc cgtttcggtg ttgaagtaaa gctccatgac gagcgtctta
     5581 gcaccgtgga ggcacgttcc ggtctgtttg aacagggcgg ctatcgggcg ctcaacaaag
     5641 gcaaagttga ctctgcctct gcggttatca ttctcgaaag ttatttcgag cagggatatt
     5701 aaggcgattt acgtcatcgc tccttattgc cggatgcggc gtgaatgact acgtttgtag
     5761 gcctgataag cgtagcgcat caggcatatt tactaaaata atcaatcact ttagcctcac
     5821 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccacctgc
     5881 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
     5941 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
     6001 tccagctttt gtgacaacac tgcccgtaaa ctccccgcca gttgattacg tacgggatct
     6061 ttttcctgcg ccggaaatga atccaccagc cgctcaactg cctgcgccgc accacgcgta
     6121 tgtaatgttg ccagcaccaa atgcccggtt tctgccgccg tcagtgccag acggattgtc
     6181 tcgctgtcac gcagctctcc gagcaatatc acatcgggat cttcccgcaa tgcggcacgc
     6241 aatcccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
     6301 tggctggcat agagatattc aacaggatct tccagcgtca gaatatgcgc atcggcatgt
     6361 tgattgagat agccaaccat cgccgccagc gtggtagatt tgccgctccc cgtcgccccc
     6421 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggcgcacca
     6481 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
     6541 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac agcaaaatcc
     6601 aactgaccat tctccagcaa tattgtccgc tgatcgtcat ccagccactc ccgcagtagc
     6661 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc gcccgcgaat acgccatcgt
     6721 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
     6781 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
     6841 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
     6901 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
     6961 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
     7021 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
     7081 aagccgcctg gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
     7141 tacccgcctc aacgatcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
     7201 taatattagt gacgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
     7261 agctgcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcacc
     7321 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
     7381 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
     7441 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
     7501 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
     7561 tcaacggtca ttgagctgta taccatggtg ctgttattac gcatctggat gcagtgggct
     7621 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtaacgca gccaattatc
     7681 gggccactgc gccgcgttat tccggcaatg gggcccattg acagcgcctc actgctggtt
     7741 gcgtacattc tcagtttcat caaagccatc gtgctgttta aagtggtgac cttcctgcca
     7801 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcctgct gattttctgg
     7861 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg ggcgtagccc gattgaatac
     7921 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcctgct accggcaatg
     7981 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgtcat caatatgggt
     8041 gtcgcagaag tcttgcaggc gaccggaaat atgctgctgc cggggctgtg gatggcgtta
     8101 tgagtgccgt aacagtgaat gatggcggtc tggttttacg gctctatatt cagccgaaag
     8161 ccagccgtga ttctattgtc ggtttacatg gcgacgaggt aaaagtcgcc attaccgcgc
     8221 cgccggttga cggccaggcc aacagccatc tggtgaagtt tctcggtaag caattccggg
     8281 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
     8341 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
     8401 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
     8461 agcgacttcg gtcttgatat cgtggcccaa acagacctcg gtgtagattc cgctgaagaa
     8521 actggcctga cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
     8581 ggtttaccgg caattgccga cgactccggt ctggcggtag atgtacttgg cggtgcaccg
     8641 gggatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
     8701 ctgctggaaa caatgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
     8761 ctggtatatc tgcgtcacgc ggaagatccc actccgctgg tgtgccacgg tagctggccg
     8821 ggcgtgatta ctcgtgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttt
     8881 gttccttccg aagggaaaac cgctgccgaa ctgacccgcg aagaaaagag cgccatttcc
     8941 caccgtggtc aggcattgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
     9001 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
     9061 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgcttaacga
     9121 tctggacaac gatgtggctt acgctcaggg ccgtgaagtg cagacaatct ttattggcgg
     9181 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctggacg gcgtgcgtgc
     9241 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
     9301 agcggatcgc tttgtcgatt atcagcgtgc tggtgtgaac cgcatctcta ttggtgtgca
     9361 gagttttagc gaagaaaagc tgaaacgact cgggcgtatt catggcccgc aagaagcgaa
     9421 acgagcggca aagctggcga gcggtttagg gttacgtagt tttaaccttg atttgatgca
     9481 tgggctgccg gatcaatcac tggaagaggc gctaggcgat ctgcgccagg ctattgaact
     9541 gaatccgcca catctttcct ggtatcaact gaccatcgaa cctaatacgc tgtttggctc
     9601 gcgccctcct gtactgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
     9661 gttattaacc gcagcgggct atcagcagta tgaaacatcc gcttacgcca aacccggtta
     9721 tcagtgccag cacaatctca actactggcg atttggcgac tacatcggta ttggctgcgg
     9781 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacca aaacgcgtca
     9841 tccgcgtggt tttatgcagg gaagatatct ggaaagccag cgtgatgtcg aagctgcaga
     9901 taaaccgttt gagttcttta tgaatcgctt ccgtctgctg gaagccgcgc cgcgcgtgga
     9961 gtttatccaa tatactggcc tttctgaaga agttattcgc tctcagttag acgaggccat
    10021 cgctcaaggt tatctcacag aatgtgcgga ttactggcag ataaccgagc atggtaaatt
    10081 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    10141 gcattaat
//
LOCUS       DACADA010000156         9961 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.158, whole genome shotgun sequence.
ACCESSION   DACADA010000156 DACADA010000000
VERSION     DACADA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.158"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..743)
                     /gene="intI1"
                     /locus_tag="ISB17_003901"
     CDS             complement(<1..743)
                     /gene="intI1"
                     /locus_tag="ISB17_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAP1981783.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWLWFWVFAQHTHSTDPRSGVVR"
     gene            901..1374
                     /locus_tag="ISB17_003902"
     CDS             901..1374
                     /locus_tag="ISB17_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HAP1981784.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1505..2293
                     /locus_tag="ISB17_003903"
     CDS             1505..2293
                     /locus_tag="ISB17_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HAP1981785.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2499..2846
                     /locus_tag="ISB17_003904"
     CDS             2499..2846
                     /locus_tag="ISB17_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HAP1981786.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2840..3679
                     /gene="sul1"
                     /locus_tag="ISB17_003905"
     CDS             2840..3679
                     /gene="sul1"
                     /locus_tag="ISB17_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HAP1981787.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3807..4010
                     /locus_tag="ISB17_003906"
     CDS             3807..4010
                     /locus_tag="ISB17_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981788.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4166..5371
                     /gene="chrA"
                     /locus_tag="ISB17_003907"
     CDS             4166..5371
                     /gene="chrA"
                     /locus_tag="ISB17_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HAP1981789.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5382..5687
                     /locus_tag="ISB17_003908"
     CDS             5382..5687
                     /locus_tag="ISB17_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1981790.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5731..5796
                     /locus_tag="ISB17_003909"
                     /pseudo
     CDS             5731..5796
                     /locus_tag="ISB17_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5914..6678
                     /locus_tag="ISB17_003910"
     CDS             5914..6678
                     /locus_tag="ISB17_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP1981791.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7171..7755)
                     /locus_tag="ISB17_003911"
     CDS             complement(7171..7755)
                     /locus_tag="ISB17_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1981792.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7755..8993)
                     /locus_tag="ISB17_003912"
     CDS             complement(7755..8993)
                     /locus_tag="ISB17_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1981793.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8990..9895)
                     /gene="mph(A)"
                     /locus_tag="ISB17_003913"
     CDS             complement(8990..9895)
                     /gene="mph(A)"
                     /locus_tag="ISB17_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP1981794.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2071 a         2880 c         2936 g         2074 t
ORIGIN      
        1 cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac
       61 agccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg
      121 ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc
      181 aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc
      241 cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca
      301 ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga
      361 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
      421 cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag
      481 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
      541 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
      601 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taaactgtaa
      661 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      721 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
      781 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
      841 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt tagccattaa gggagttaaa
      901 ttgaaaatat cattgatttc tgcagtgtca gaaaatggcg taatcggtag tggtcctgat
      961 atcccgtggt cagtaaaagg tgagcaacta ctctttaaag cgctcacata taatcaatgg
     1021 ctccttgtcg gaagaaaaac atttgactct atgggtgttc ttccaaatcg caaatatgca
     1081 gtagtgtcaa agaacggaat ttcaagctca aatgaaaacg tcctagtttt tccttcaata
     1141 gaaaatgctt tgaaagagct atcaaaagtt acagatcatg tatatgtctc tggcgggggt
     1201 caaatctata atagccttat tgaaaaagca gatataattc atttgtctac tgttcacgtt
     1261 gaagtcgaag gtgatatcaa attccctata atgcctgaga atttcaattt ggtttttgaa
     1321 cagtttttta tgtctaatat aaattataca taccagattt ggaaaaaagg ctaacaatgc
     1381 gttgcagcac cagtcgcttc gctccttgga cagcttttaa gtcgcgtctt tgtggttttg
     1441 ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg
     1501 catcatgggt gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt
     1561 gatcgagcgc catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga
     1621 tggagggctg aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa
     1681 cgattcgctc cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga
     1741 tggcggaaca tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg
     1801 gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc
     1861 cggaacgttc gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag
     1921 gcaacacagc cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa
     1981 ggagcatttc tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg
     2041 gaagggtgac gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac
     2101 tggtctcatt gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga
     2161 gcatcggccc ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct
     2221 agcaatgcgc gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag
     2281 aatcttgcgt tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg
     2341 cctaactcgg cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac
     2401 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     2461 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
     2521 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
     2581 ctaagcttgc cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt
     2641 ctctggttct gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg
     2701 tcgtcataat tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct
     2761 ttgtaggtat ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt
     2821 cgctgcggag gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc
     2881 cttcttcgat gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat
     2941 gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag
     3001 gcctgtatcg ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga
     3061 tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa
     3121 gcgcggcgtg ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga
     3181 tattgctgag gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc
     3241 cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga
     3301 ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc
     3361 ggggatggga tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct
     3421 tcaaaagctg aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt
     3481 cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga
     3541 acttcacgcg atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg
     3601 aagcgcaatc accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg
     3661 agggttagat catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt
     3721 tccgcgaagg tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg
     3781 cggttcatac cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg
     3841 cctgctcggg tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt
     3901 ccgccaaggg aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct
     3961 tcgtcctgag atccgagctc ctagggatcg cctcagaaaa cggaaaataa agcacgctaa
     4021 gccgtaagta agcgtgctcc tgtgaaagcc acagctaaaa ctgcgtagta cacatagagg
     4081 tatttttttc tagatttata tcgaatttcg ttatcgaatt tcgcttatta ttgatcaact
     4141 cgttgcagat catgaaggcg aaagtatgac gaataaccct accgatgaca gcagaccatg
     4201 gtcggtcttt cttatttttc tgcggcttgg attgacatct tttggcggcc ccattgcgca
     4261 cttgggctac ttccgcgccg aatttgtcac acggcggcgc tggctctccg aacggagcta
     4321 tgctgacttg gtcgcgcttt gtcagttctt gccagggcct gcaagcagcc aggtcggcat
     4381 agcggtagga ctgtctcggg ctggatacag cggggcgctg gctgcttggg ctggcttcac
     4441 gctgccgtct gccatagcct tgatcctttt tgcgctcggc atctccagct atggcgatta
     4501 cgtctcgcag ggcgcgttgc atggcttaaa agtggtggct gtggccgtgg tcgctcaagc
     4561 agtatggggc atggcgcgta acctatgcac ggatgggctg cgagtcacca tcatggcaat
     4621 tgctacctgc gtcgttttac ttgtgccgtc cgcgtgggga caggttggcg tgattgctat
     4681 cgcaggcatc gcaggccggt tattgttcaa gccagcgaaa gttgttgagc atgaccccct
     4741 acctatcacg gtcagtcacc gggccggcgt gctttggctc tcgctgttct ttgtcttgct
     4801 gattggcctg ccggtgttgg ccgaactgat gccaagtcaa accatggcaa tggtggattc
     4861 cttctatcgt gtcggatcac tggtgttcgg cggtggtcac gttgtgctgc cattactgca
     4921 agccgaagtg gtgccctccg gctgggtcaa caatgaatcc tttctcgcgg ggtacggggc
     4981 agctcaagcg gtgcccggcc ctttgttcac gttcgccgcg tttcttggtg cctcgatgaa
     5041 caccgccccg tcgggctgga tcggcggcat tgtgtgtctg ctggctatct tcgcgccctc
     5101 gttcttgctg gtcgtcggat caatgccatt ttgggagcgt ttgcgccgca atacaggcat
     5161 ccaagctgcg ctggccggga tcaatgccgc tgtagtcggc ttgctgctgg ccgcgctgta
     5221 tcagcctgta tggactagcg ccatctttca gccgcaagac ttcggcttgg cattagttgc
     5281 ccttgtcgca cttatgttct ggaagctccc gccgtggctg gttgtcctcg gtagcggtgc
     5341 agctgggtgg ctgttgagcg tcgctctgtg aggcacaaaa aatgactgac aaagacctct
     5401 acggcggttt gatccgcctg cacatccttc accatgcagc cgaggaacct gtctttgggc
     5461 tggggatcat cgaagagcta cgccgacacg gctacgagat gagcgctggc accgtgtacc
     5521 cgatgctgca cggcctggaa aaaaaaggct atctgacctc acgccacgaa cgcaccgggc
     5581 gacgtgaacg gcgtgtttat gacatcactg aacaaggcag aactgcactt gctgatgcga
     5641 aaacaaaggt caaggagctg ttcggagagt tggtagaagg tggttgagtt tttgcttcta
     5701 tgtcaggttg agctataccc taactggatt gtcattttca gaagacgact gcaccagttg
     5761 attgggcgta atggctgttg tgcagccagc tcctgacagt tcaatatcag aagtgatctg
     5821 caccaatctc gactatgctc aatactcgtg tgggctctgt tgcaaaaatc gtgaagcttg
     5881 agcatgcttg gcggagattg gacggacgga acgatgacgg atttcaagtg gcgccatttc
     5941 cagggtgatg tgatcctgtg ggcggtgcgc tggtattgtc gctatccgat cagctatcgc
     6001 gaccttgagg aaatgctggc ggaacgcggc atttcggtcg accatacgac gatctatcgc
     6061 tgggtccagt gctacgcccc ggagatggag aagcggctgc gctggttctg gcggcgtggc
     6121 tttgatccga gctggcgcct ggatgaaacc tacgtcaagg tgcggggcaa gtggacctac
     6181 ctgtaccggg cagtcgacaa gcggggcgac acgatcgatt tctacctgtc gccgacccgc
     6241 agcgccaagg cagcgaagcg gttcctgggc aaggccctgc gaggcctgaa gcactgggaa
     6301 aagcctgcca cgctcaatac cgacaaagcg ccgagctatg gtgcagcgat caccgaattg
     6361 aagcgcgaag gaaagctgga ccgggagacg gcccaccggc aggtgaagta tctcaataac
     6421 gtgatcgagg ccgatcacgg aaagctcaag atactgatca agccggtgcg cggtttcaaa
     6481 tcgatcccca cggcctatgc cacgatcaag ggattcgaag tcatgcgagc cctgcgcaaa
     6541 ggacaggctc gcccctggtg cctgcagccc ggcatcaggg gcgaggtgcg ccttgtggag
     6601 agagcttttg gcattgggcc ctcggcgctg acggaggcca tgggcatgct caaccaccat
     6661 ttcgcagcag ccgcctgatc ggcgcagagc gacagcctac ctctgactgc cgccaatctt
     6721 tgcaacagag cccgccgtgc tagtctgctc ggtgatggtg gagtgaagcc aacccgcaat
     6781 cgggttatga atctgcatcg cgattcgcaa tcagctgtct cttgagcatg tcgaactcct
     6841 gcgatgttat ctcgccgtgc tcgtgctgct acaccaactt gtccaactca tccgccacac
     6901 cgctccccac cacctgcgat ggccaggttg tttgctgggc gcattggcgg agtatcgtct
     6961 cgacccggga cacccacacc ggcacaacct tacgggagtg attcactgtc aaagaatcgg
     7021 cccggtgctc tgacgcaagt atcgggatgg tcaccatttg taagccgtag acctgagtgg
     7081 tgatcaagac ttcgatacca ccgaccgtac cggtactaat cgacgacggt cgtgttcgtc
     7141 gcctgccgca gggactctgc acacctccgt ttacgcatgt gcctggagga gttggaaatc
     7201 gtcgtgttcg ggaaacatta aacacaggat ggcagcgatc tgagccagca catgatcagc
     7261 tagctcacca tccggatcga cggcccactg catcgtcgcg ccagcgatga ccgagtgcag
     7321 gagcaactca gctgccgcag gagcacctgg gggcagtcgc ttgcggatcc cctccaccac
     7381 cgcgcggttc cgctggatcg caagcgtgcg tagctccggc acctggagct cgtaccagga
     7441 gatgagatag ttcaccgaga agtcgttgcg agtgttcatg ctccgaacga gcacctgcaa
     7501 aaattcccag agcccttgcg gccctgcgcc tatcggtatc gcattcaggt aatgccgcac
     7561 ctgctcgacg ccgcgctcca tcatcctcac cagcagcgta tcgcggttgg tgaagcgctg
     7621 gattaacgct gcgcgggaga gccccacctc ctttgctact ccgctgagcg tgaactctat
     7681 gggaccgcaa cgcttcagca ctacggtggc ggcctcgagt acctcgtcat cggacttgag
     7741 cttggggcgg ggcatcagtg ttcaccttct gtatgggttg gggcggaggc tgtggctgcc
     7801 gccgccattg tagcaaattg aagacggagc gagagtagag ccacgagccc cgcaaacacg
     7861 gccgatacaa cgaggccagg gagcggacca gcaaggtcga caaacgcgcc ggccgcaagc
     7921 ataaccatgg gcgaggctga cagcatcacc gccgagaccg tgccgagtac ccggccgaga
     7981 agttctggcg gcgtgcggtt gtagatggca gcgttgagaa tgggagagac tgagccggtc
     8041 agcagtccca cgagcgcgcc caacaacatc agcaccggca cgcctggcaa ctgtgaaagc
     8101 agaagcgagc ccaccgcaga gccacaaaat gccaccgcca gccagttctg cgctgatatc
     8161 cgggcgccga ccgacgcatg aatggcaatg ccaaggagac caccagcccc catcattgag
     8221 gagaacagcc cgagctctgc tacttggcgt cctgcatcta caaacagcgc aggcatgatg
     8281 acgctgccgt tggcgccaac gatgcccacg aagatcatca ctataccaaa gagagggcgc
     8341 agcaggggtt cgctccagag aaaagcgacg ccggcgcgca tggagagagt cgccgtcgtg
     8401 gtcatcgtcc gagcggcacg cgcgggaagc acccacgcgc cgagcagacc tgcaaggacg
     8461 gagcagaacg ccgtcagccc gagcgttggc gcagcgccaa gcaggccgat tgcggccccc
     8521 ccaagggccg ggccacctag aatcgcgacg ttcccgatca ccgctttcag tgacgagacg
     8581 cgctcaacgg agagcccggc gacgtggccg agtttgggca gctcactgtc ctgcgcggcc
     8641 ataccgggtg cgtcgaacgc ggcaccgagc accacgcaag cgatcagccc agtgttcgag
     8701 agggcgccaa cggcatcgag cagtgggatg ctcgccatgg ccacgccgcc caccacaccc
     8761 gagatcaatg cgacgggcgc gcgcccgaac cgatcgacga ggccaccacc aacccacgcg
     8821 ccgatgatgg tcgcgatgac gctgctagcg gccgtggcgc ccgcccaggc cgcgctcttt
     8881 gtatgagaca ggacgaacca tggaagcgcg agggccgcca ccgcgttgcc gatccggaag
     8941 agaaaggtcg ccgcgaacag cgtcgcgagc gggctatatc gacgttcgct cattccgctg
     9001 cggcgagctg cgccttcgcc gcagcgaggt actcttcgtt acccgagtcg agggcgaaga
     9061 gtgcgtaggt gaccgccccg aacgcaaggc gctccgcgat gtggtgggcg agccgcggcc
     9121 acacccggcc accggccgct tcatacgtga ggaggagctt cgcgagcccc tcttcaccaa
     9181 agaccataag gtgcgcggcc atgtcgatgg cagggtcatc aacgcgggcc tcgctccagt
     9241 cgatcatccc gctgacgcgc tccgtgttgt cgatgagcac atggcccacg tagagatcgc
     9301 catgcaccac cacggagaaa tctggccacg acgaatcgtc gtcgagccag cgctgccacc
     9361 ggtggaggcg cttgtcgttc accacgaact cgcgtcggac gcggtcaacg tcgtcggcca
     9421 ccttctgacg ggcctgcgtc ggtgtacgga tgagcatccc cgcatccacg gcggcggaaa
     9481 tggggacggc atgcagggcg gcgagcgcgg tcgcgaagct ctccgcgaag acctccgagt
     9541 cctgcggcac gacccagtcg ggcgtggacg aaccaggctg gatgaccatc gcagtcgagt
     9601 cttcgagcat gggataggca acgagctcgg cgttggccac gcgccagtcc ggcaccgcga
     9661 acggcaggcg attcttgagc attgccagca cccgcgcctc tggttcgacc ttcgcgctta
     9721 cctcggctcg gcgcgggatg cgcagcaccc accgacgtcc atcgtcgacg gtggcgatca
     9781 cgatcctata gtcgagccca agctcattga cagtcagcgg gccatggagc ttgagcccat
     9841 gtcgggctgc aagtgcgtac agttgggagg tatcggcggt cgtgactacg gtcatgattc
     9901 actcctgagg gcttgacggg tttagccacc taaatgtaac agtcacgtcg gttatattca
     9961 a
//
LOCUS       DACADA010000157         9934 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.159, whole genome shotgun sequence.
ACCESSION   DACADA010000157 DACADA010000000
VERSION     DACADA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.159"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(203..394)
                     /locus_tag="ISB17_003914"
     CDS             complement(203..394)
                     /locus_tag="ISB17_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HAP1981795.1"
                     /translation="MGINAVVMDGVVIGENSIVGVSAFVKAKAEMPTNYLIVSSPAKA
                     IRELSEQELALRKQGIRCW"
     gene            411..680
                     /locus_tag="ISB17_003915"
                     /pseudo
     CDS             411..680
                     /locus_tag="ISB17_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(852..1178)
                     /locus_tag="ISB17_003916"
     CDS             complement(852..1178)
                     /locus_tag="ISB17_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAP1981796.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(1186..1371)
                     /locus_tag="ISB17_003917"
     CDS             complement(1186..1371)
                     /locus_tag="ISB17_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAP1981797.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1368..4007)
                     /locus_tag="ISB17_003918"
     CDS             complement(1368..4007)
                     /locus_tag="ISB17_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAP1981798.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            4215..5204
                     /gene="ldhA"
                     /locus_tag="ISB17_003919"
     CDS             4215..5204
                     /gene="ldhA"
                     /locus_tag="ISB17_003919"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP1981799.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5315..5737
                     /gene="hslJ"
                     /locus_tag="ISB17_003920"
     CDS             5315..5737
                     /gene="hslJ"
                     /locus_tag="ISB17_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAP1981800.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5734..6000)
                     /locus_tag="ISB17_003921"
     CDS             complement(5734..6000)
                     /locus_tag="ISB17_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAP1981801.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            6274..9798
                     /gene="nifJ"
                     /locus_tag="ISB17_003922"
     CDS             6274..9798
                     /gene="nifJ"
                     /locus_tag="ISB17_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP1981802.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2499 a         2578 c         2413 g         2444 t
ORIGIN      
        1 tcatttgata aaaaacggta caaatcaata ctcaaataaa taaatcagag cagaagcata
       61 attctgcttt ataattaaag ggaaaaataa ttatataact gttactgttt cggtcgcaga
      121 ttctcatcaa acaccaggcg tttcctgcca ggttcaactt cccgcaatgg ctcgacttga
      181 tgcaacgtct gcttacaacg agtcaccagc acctgatgcc ctgctttctc aatgccagct
      241 cctgctcgct gagttcacga atcgctttcg ccgggctgct gacaatcaag taattagtcg
      301 gcatttctgc tttggctttc acaaatgcgg aaacaccaac aatactgttc tcaccaatca
      361 ccacaccgtc catcactacc gcgttaattc ccactaatgt atggcggtgg ttaatgcagc
      421 cgtgaagaat agtcggagat acaggtatgt cagtacgtag tttgtaccga atgtttgccg
      481 ataaaggttt ggtggtcgcg caatatattc gtaaccgtcg tctcgatttt tgtgcagatg
      541 cgattcgcca tgccgcagat gatgaaaaac tggcaggcat tggctttcat tggggatttt
      601 ccgaccagag tcatttttca acggtattta aacaacgctt tgggatgacg ccaggcgaga
      661 atcggcgtaa attccgctaa ctaattcgcg ttgcatgtac agcaacgcga attagttaaa
      721 acagatattt gactatgcat ttttcagggc gaaaggcaat ttgacaggcg tttttgaata
      781 atcatcttgt ttccggaaaa tagattaatc gccttctcct gtagcgttat cccgatattg
      841 cggctttact attaaaattt tcgcacccat ttaccgttta tcccttgtac atattccccg
      901 ggtttggccc gttctacgag cttttgcccg gcgagtttcg cgatatcatc taccgacaca
      961 ttattttgct ttgccagttg ttgatagctc gctttacgtt cggcattaat gtcggttact
     1021 aatttctcag tttcagcatc tgttttcagc gcaaccagat aaccataaaa tgtttcgcct
     1081 acccgcccct gagttctggc ttcatccaga gtcaatgcca ttacattgct gcttaccaag
     1141 ccaacaagaa atgcacaaag aagtaatgtt cttttcatca tcacctcaaa aaagatcgct
     1201 acgggtctca agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat
     1261 tttaacgttc atattgatag tgatcggttc cttaggtgca gcgacttcaa tacgaggcgt
     1321 acagccaacc agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttatcc
     1381 tcacactctt ttccttgcgg acagtcattt cccggcagac gcgcgttctg ctccagccat
     1441 gcctggagat tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga
     1501 tggtaattaa gatttaccgt accgctttta ccatcaacgc gactggtacc tgtaatattg
     1561 gcctgcatgg ttaataaacc gagattatct aaattaattt ttgtcgatga acggctaatt
     1621 tccatatagc gcaaccagtt aattgctgaa cccgcggtca tattgtcttt caccaccgca
     1681 tctgcggtgt ctttatccag ccgcaaggtc attggtccgc tatttgccag ccagccgtct
     1741 ttcactatcc atttttcatt gtttaaccat aacggcagtg caccactaaa tgccccggac
     1801 atggcgaatt gtttcggatt aacggcgcta accagttcgc tggatgagag attattcagt
     1861 cgcaacagtg ccgggtcatg ttgcggcata cgtaattgtt tcatcagcac gttaccgcct
     1921 aacacatcga cactaacatc agtcaacagc aatggttctt cttcagtcca cggataacgc
     1981 ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc
     2041 aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg
     2101 acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaatacg
     2161 ccgtgccccc ccgcgcggaa tccttgttcg ggcgctgctg aaaatgcgac ctgagcatac
     2221 agttcaccat cgcgtaagtt catcttccag tcgggtggcg ccaacggctg gaatacagtc
     2281 agtgattgtt taggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt
     2341 acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa atacgtcgga
     2401 tcgcgcccat cgacactaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac
     2461 agcgtttgcc cggcgtccag tgacagagcg ccgctgaagg agggatgctg tgcgtcacgt
     2521 ccccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat
     2581 tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca
     2641 ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactcccttt tccccagtag
     2701 cgccactgcc agcggccagc gtcagggaga aaatcattcg ccagcccatc catatgcagc
     2761 acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca
     2821 ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa
     2881 tcaatgactc ttccctttga acgaagtaac gcgccaggct caaaggtcag cgttgggtca
     2941 gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc
     3001 gcttcaccgg tcagctgcag aggcagctgg ctgttatcca tgcttaattt tcctgggcca
     3061 aaatttaaca ccgcgttgcc tttacccgct tgcccctggg tcagcacact cagtcgcccg
     3121 ctgaccagag cgttctcgag ccctgcctgc cagttgtcga ctttgacacc cagtcggcca
     3181 ctcaaaggaa aacctgcata cggccagctc cagcgaccat cgcttacggt caattgttga
     3241 cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc cagcacaatc
     3301 aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgaagg ctcctgcggt
     3361 aagtttaacg tcgcagtagc atgaccactt acaggaagtc catccggcac gagcggcata
     3421 gtaaattccc ccaccagttt taccggcggc tgattttcaa acgcgacgac atccagttcg
     3481 ctgactgtaa gttgttgccc tttaagctgg ccttgaaatt taactttttc gccctgataa
     3541 cgcagttgct ggatatcgga ggttaatgcg agagagagtt ttccctgcca ttcctgccag
     3601 ggagaaaaaa tcagtttatc gatattgatc caggtgttag gcaacatggc ctgccactgc
     3661 gcgagggttt tgggagcggc tggcgattgc tccgtctgcg gcaatttcgc cagacaagca
     3721 gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg
     3781 ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg gatgattaaa
     3841 cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag
     3901 atgcccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga
     3961 ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg tagttaatat
     4021 cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag
     4081 ttgaaggttg cacctacact aagcatagtt gttgatgaat ttttcaatat cgccataact
     4141 ttcaattata tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca
     4201 ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc
     4261 tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg
     4321 aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg
     4381 gcagccgccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct
     4441 gtgccggttt caataacgtc gaccttgacg cggcaaaaga actggggctc aaagtagtcc
     4501 gtgttccagc ctatgatcca gaggccgtag ctgaacacgc catcggtatg atgatgacgc
     4561 tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc tctctggaag
     4621 gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt gatcggtacc ggtaaaatcg
     4681 gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt
     4741 atccaagtgc ggcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct
     4801 ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac catctgttga
     4861 acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg
     4921 cattgattga ttctcaggcg gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg
     4981 gtatggacgt gtatgagaac gaacgcgatc tgttctttga agataaatcc aacgacgtga
     5041 tccaggatga cgtattccgt cgcttgtctg cctgccacaa cgtgctgttt accgggcacc
     5101 aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa
     5161 gcaatctgga aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg
     5221 cattccaggg gagcttattc agataatccc caaagacctt tcatcctcta ttcttaaaat
     5281 agtcctgaat cagaaacagt aattgagaac cacaatgaag aaagtagccg cgcttgttgc
     5341 gctaagcctg ctgatggcgg gatgtgtaag tagtgacaaa attgctgtaa cgccagaaca
     5401 gctacagcat catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa
     5461 aaatccgcca gaaatcagct ttggtgaaaa gatgatgatt tccggcagca tgtgtaaccg
     5521 ctttagcggt gaaggcaaac tgtctaatgg tgaactgacc gccaaagggc tggcaatgac
     5581 ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct
     5641 gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca
     5701 gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc
     5761 aagtgactgt tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc
     5821 atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact
     5881 actggacatc gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg
     5941 ttcacgacta cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat
     6001 cttttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag
     6061 ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta tggctatttt
     6121 tttgccctct gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa
     6181 tgcagaagaa agccatttgc taaaattgaa cgattactac tgggcgcgca gcaatttcgt
     6241 gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg
     6301 gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt
     6361 tccacgatgg cagaacaggc tgatgcatgg gccggaaacg gcttgaaaaa cgtttgggga
     6421 gacacaccac gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggt
     6481 gctttgcaaa ctggtgccct ctcaacatcg tttacgtcat cgcagggttt gctgctgatg
     6541 atcccaacgc tgtacaaact ggcaggcgaa ctcacaccgt ttgtcctgca tgttgcggca
     6601 cgtaccgttg ccacacatgc actctctatt tttggcgatc attccgacgt tatggcagtg
     6661 cgccaaacgg gttgcgcgat gttgtgtgca gcaaacgtcc aggaagcgca agactttgct
     6721 ctcatttcgc acattgcgac gctgaaaagt cgcgtgccat ttattcattt ctttgatggt
     6781 ttccgcacat ctcacgaaat caataaaatt gtcccgctgg ccgatgacac gattcttgaa
     6841 ctgatgccgc aggcagaaat tgatgctcat cgcgcccggg cactcaaccc ggaacatccg
     6901 gtgatccgcg gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac
     6961 ccatggtaca acgcggtcta tgaccatgtt gaacaggcga tgaatgattt cgctgccgcg
     7021 acaggtcgtc agtatcagcc gtttgagtat tacgggcatc cgcaagcgga acgggtgatt
     7081 atcctgatgg gctctgccat tggcaccagt gaagaagtgg ttgatgaatt gctgacccgt
     7141 ggcgaaaaag tcggcgtgct gaaagttcgc ctgtaccgcc ccttctccgc caaacacttg
     7201 ctgcaagctc tgccgggatc cgtgcgcaac gtggcggtac tggacagaac caaagaaccc
     7261 ggtgcccagg cagaaccgct ctatctggat gtgatgactg cactggcaga agcctttaat
     7321 aatggcgagc gcgaaaccct gccccgtgtc attggtgggc gctatggtct ttcatccaaa
     7381 gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa
     7441 gcgcgcttta cggttggtat ttacgatgat gtaaccaatc tgtcactgcc gttgccggaa
     7501 aacaccctac caaactcggc gaaactggaa gccttgtttt atggccttgg tagtgatggc
     7561 agcgtttccg cgaccaaaaa caatatcaag attatcggaa attccacgcc gtggtacgca
     7621 cagggctatt ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc
     7681 gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt tgttggctgc
     7741 caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc tggcggcatt
     7801 ttcctgctca acacgccgta cagcgcagct gaagtgtggt cgcgcttgcc gcaggaagtt
     7861 caggccgtgc taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc
     7921 cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg
     7981 acgcagattc tgcctggcga tagtgccctc gcagaattgc agggtgcgat tgccaaaagt
     8041 tacagtagca aaggccagga tctggtggaa cgcaactggc aggctctggc gctggcacgt
     8101 gaatccgtag aagaagttcc actgcagcca gttaatccgc atagcgccaa tcgaccgccg
     8161 gtggtttccg atgccgctcc tgatttcgtg aaaaccgtaa ccgctgcgat gctcgccggg
     8221 cttggcgacg ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcacc
     8281 acgcgctggg aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt
     8341 acccaatgta accactgtgt cgccgcttgc ccacactcag ctattcgcgc aaaagtggta
     8401 ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt
     8461 gatatgcgcg ggcagaaata tgtcttgcag gtggctccgg aagattgcac cggttgtaac
     8521 ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat
     8581 atgatgtcgc gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac
     8641 ctgccagaaa tcgatcgtag caaactggaa cgtattgata ttcgtacatc gcagctaatc
     8701 acgccgctgt ttgaatattc cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa
     8761 ttgctgactc agctctatgg cgaccggatg ttgatcgcta atgccactgg ctgttcttca
     8821 atatacggcg gtaacctgcc ttcaacacca tataccaccg atgccaacgg tcgtggaccg
     8881 gcatgggcga actcgctgtt tgaagataac gccgaatttg gccttggttt ccgcctgacg
     8941 gtcgatcaac accgtgtccg cgtgctgcgt ctgctcgatc aatttgccga taaaatcccg
     9001 acggaattac tgactgcgtt gaaatcagac gccacgccag aggttcgtcg tgaacaggta
     9061 gcagctttac gccagcaact caacgatgtt gccgaagcac atgaactgct gcgtgacgca
     9121 gatgcactgg tggaaaaatc aatctggctg attggtggcg atggctgggc ttacgatatc
     9181 ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg
     9241 gatacgcaat gctattccaa caccggaggt caggcatcga aagcgacacc gctgggtgca
     9301 gtaactaagt ttggcgagca tggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg
     9361 atgatgtacg gtcatgttta tgtggcgcag atttctctcg gcgcgcagct gaaccagacg
     9421 gtgaaagcga ttcaggaagc ggaagcgtat ccggggccat cgctgatcat cgcttatagc
     9481 ccgtgtgaag agcatggtta cgatctggca ctcagccacg atcagatgcg ccaactcact
     9541 gctaccggct tctggccgtt atatcgcttc gatcctcgtc gtgccgatga aggcaaactg
     9601 ccgctggcgc tggattcacg cccgccgtca gaagcacttg aagaaacgtt acttcacgag
     9661 cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat
     9721 gctgcagctg atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa
     9781 aaaagcaaca ccgattaaat gctctggata aggattatcc aattcaaaaa aaaagcccgg
     9841 acgactgttc gggcttgtct tcttatgtgt tggaaaatca gtagcaatgc aatggcccaa
     9901 cagaaaataa aatctgtgac aataaaggca tata
//
LOCUS       DACADA010000158         9845 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.160, whole genome shotgun sequence.
ACCESSION   DACADA010000158 DACADA010000000
VERSION     DACADA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.160"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..411)
                     /gene="folK"
                     /locus_tag="ISB17_003923"
     CDS             complement(<1..411)
                     /gene="folK"
                     /locus_tag="ISB17_003923"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAP1981803.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELV"
     gene            complement(408..1826)
                     /gene="pcnB"
                     /locus_tag="ISB17_003924"
     CDS             complement(408..1826)
                     /gene="pcnB"
                     /locus_tag="ISB17_003924"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAP1981804.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1865..2791)
                     /gene="gluQRS"
                     /locus_tag="ISB17_003925"
     CDS             complement(1865..2791)
                     /gene="gluQRS"
                     /locus_tag="ISB17_003925"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAP1981805.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQNIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2828..3283)
                     /locus_tag="ISB17_003926"
     CDS             complement(2828..3283)
                     /locus_tag="ISB17_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAP1981806.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3461..4165)
                     /gene="sfsA"
                     /locus_tag="ISB17_003927"
     CDS             complement(3461..4165)
                     /gene="sfsA"
                     /locus_tag="ISB17_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP1981807.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4180..4710)
                     /gene="thpR"
                     /locus_tag="ISB17_003928"
     CDS             complement(4180..4710)
                     /gene="thpR"
                     /locus_tag="ISB17_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAP1981808.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4784..7213
                     /gene="hrpB"
                     /locus_tag="ISB17_003929"
     CDS             4784..7213
                     /gene="hrpB"
                     /locus_tag="ISB17_003929"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAP1981809.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTQMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFLRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPRLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7307..9832
                     /gene="mrcB"
                     /locus_tag="ISB17_003930"
     CDS             7307..9832
                     /gene="mrcB"
                     /locus_tag="ISB17_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAP1981810.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
BASE COUNT         2258 a         2512 c         2815 g         2260 t
ORIGIN      
        1 gaccagctcc ggcgcaattt caaacagtgg ccacagcata aagccacgat ttttcatatc
       61 gtagtgcgga acggtcagac gttcggtatt tattacttca ttaccaaaca gcatgatgtc
      121 gagatccagc gtgcgcggtc cccagcgttc agctttacgg acgcgacctt gctgcaattc
      181 gatacgctgt gtgtgattga gtagctcttc aggggcaaga gtggttttca gcgcgacggc
      241 ggcatttaag taatcgggtt gatcttgcgg ccccagaggt ggggtgcggt aaaacgaaga
      301 aacggcaaga atgcggcttt ctggaatatc gcctaatgct ttcagggcag cattgacctg
      361 ctccagcgga gaggccagat tgctgcctat ggcaatatac gccactgtca tgtagtaccc
      421 tcacgacgcg gtgcgcgttt gcgtggacga cgagtacgac gacgcggtga cggttcttca
      481 tccagttcgt taagcatccc tttttggtct ggcggcgcgg aaacctggaa ctcaccccac
      541 catttcacca gacgttgcag ttcagcgtta cgctcaactt cggctcgtaa ggccaacagg
      601 tcgtatgccg cacggaactt aggatgctcc agcagtttcc atgcgcgttt accctgacga
      661 cgggacatac gcaactgcaa ctgccagata tcgcgagtta gcgtagtcag acgtttcggg
      721 atcgccagtg aacggcaggc ttcgtccagc acgtcgttca tcgccagtgc gaaagcgtcg
      781 tgataggtca ggccgctctc ctgagcaatt ttctgtgccg tctccagcaa tgggtaccag
      841 aacatggcgg caaacaggaa cgccgggttc acgcgcatat cgttatggat acgcgtatcg
      901 gtattcttca gtacctgctc aataatgcgt tccatcgggc tgtcgccatt ttccgtgaag
      961 tagcgggtaa tggtcgggaa cagcggctgg aacagatgat attcacacag cagcttatag
     1021 gtttcgtaac cgtagcccgc ttgcaacagt ttcagcgatt cttcaaacag acgtgccggt
     1081 gggatatcgt tcagtaaggt tgccaggcgt gggatcggct cggcggtttc cgggctgatg
     1141 cgcatgccca atttggcggc aaaacgaacc gcgcggagca tacgtaccgg atcttcacgg
     1201 tagcgcgttt ccgggttacc aatcagacgg ataacgccgt ctttcagatc cttcatgccg
     1261 ccaacgtaat cacggacggt aaaatctgcc acgctgtaat acaggctgtt gatagtgaaa
     1321 tcgcggcgct gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag caacataccg
     1381 ttttgcccgc gttgggaggt cgtgcggtcg ctgacgttac cttcgtgatg tccacggaag
     1441 gtcgcaactt cgataatctc cgggccaaac atcacatgag ccagacggaa acggcgaccc
     1501 accagacggc agttacggaa cagtttacgt acctgttcag gcgtggcgtt agtggttacg
     1561 tcaaaatctt tcggcttttt gccaagtaac aggtcgcgca cgccgccgcc aaccagccag
     1621 gcttcgtatc ccgctttatt gagcctgtac attaccttca gggcattttc actgatatct
     1681 ttgcgggaaa tagcatgctg ctcacgcggg atcaccgtca cctgtggacg ggcgactgcc
     1741 tgttcagcct cgctttcctc gcggcttagc accttgcggc aaaaattagc gactcgggta
     1801 aaaatagtac acctcggtag tgtcaaacat cattcaggac aaaaaaatag cggctaatca
     1861 tagctcagca tgacgcattt gagaatgttg aatttacaat tgccgactca ggcactgcgg
     1921 tgagccgcca gtttttgacg gctgactgaa ggatttgctc gacgctgaaa tcctgccagt
     1981 gtgtttctac ctgctgaccc agaaaatgaa gtgccgcgat taacaccggg cgcggatcgc
     2041 ctttcggcaa cgcaggcgca tgattctgct tggaaagttt agcgccttgt ggattaagcg
     2101 ccagcggtaa atggatgtaa tctggcactt tccagccaaa aagctggtac agcgagattt
     2161 gccgcacagt cggttcgatc aaatctgccc cgcggacaat ttcgctcacg ccctggaagt
     2221 gatcatccac caccaccgcc aggttatagg caaacaaacc atcacggcga tgaataataa
     2281 aatcttcccg tgccagtttt tcgtcggcgt gaataatgcc tcgcagcagg tcagtaaact
     2341 gggtgactgg atgctgctgg cggatacgta ctgcggcgtt gtctggtcca tgatgcaaat
     2401 cccggcaatg accgtcgtaa ataccgccaa tgttttgaat acgcgcacgc gtgcaggtgc
     2461 agtaataact tagtccttgc tcatgtaacc aggcgagtgc ttcgcgatag gcgtggtgac
     2521 gttgcgattg ccagagaacg tcgccgtccc agtgtaggcc gtaatgttcc agctggcgca
     2581 ggatagtctc tgcggcaccg ggaacttcac gaggcgggtc gatatcttca atgcgtacca
     2641 gccagcgacc ttgccgggcg cgagcctgca aatagctgcc aagcgcagca atcagagaac
     2701 caaaatgaag ctcgccggaa ggagaggggg cgaagcgacc aatatagtgt gtgtctgtca
     2761 tctctttgaa caaaaaataa ggcgggagca tttcccgcct gtggtaaaca tgatggaacg
     2821 gctgtaatta gccagccatc tgtttttcgc gaatttcagc cagcgtcttg cagtcgatgc
     2881 atagatcggc tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca ccgcaggatt
     2941 cgcagtagcc gaaatcttcg tcttccactt ttttcagcgt cttctcgatc tttttgatca
     3001 gcttacgctc gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc tgggctgcac
     3061 ggtctaccgg gtccgggaag ttggctgctt catcctgcat atgtgtaacg gtgcgatcga
     3121 cttcatccct gagttgatta cgccatgctt ccagaatacg acggaagtgc gccagctggg
     3181 cttcattcat atactcttcg cccggcttct cctgatatgg ttccacccca gcgatggcga
     3241 gaatactcag ggacgatgtt ttacggtttt gcccttcttg catgttgctt ctccttaaca
     3301 cgcactatcg atccccatgt tcgggggaaa aatgaggccg ctataaatag cagatgcttt
     3361 tccggatagc aattatctaa acgtaacact tgacaactgt gtgaggaaaa gcgtatttgc
     3421 gcacgcgacc agaatgtaaa ttaaccagtt acttactcta ctacaatgta accggtagtg
     3481 attttttaag agccatgcct tcggcagaaa gttccgcttt gtaagccaga atttctaccc
     3541 ccctctgttg agcttctgac aatagttgtg cgtatttctc atcgatgtgg cgcgcgggtg
     3601 aaaaccgtgt aatggctgaa tgcagcacgg cgaaaaagat aaccgcacgc tggccttcag
     3661 ccgctacgct catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc gcatcgggaa
     3721 aatatccctg ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag cagtctggac
     3781 gcgaatccgc ctgcaacata aagtcaatac ggctgcgttc ggcgccgtat tttacttcgc
     3841 ttttcagcga gctatagcct gacagctctg aaattgattc attaaggata gcctctttcg
     3901 tcaacctgtt agcccaaagc gtattgacgc aaataattgc gccgctttgg ctttgagtta
     3961 attcccaggt gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac cagacggtat
     4021 cgccaggcgt tgcacaaccg gtcatcgcac ccgtattcgg gcagtgtagc gttaattcgc
     4081 gaccatcggg tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt agcgtcgcgc
     4141 gctgtagagg gggagaaaat tccattgcga caatccttgt tattgcgtta gcgcccagcg
     4201 ttttagcggc gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa gggtgaactc
     4261 cgtcaccgta tacgaccagt taaaacctgg cggcgggatt gtcaccgcct cgctggcgtc
     4321 gcgcaataag gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac cgctgcgggc
     4381 agcctgtgaa cggagcatat tcgccagctg gattaagccg cgtggcggct gtcgcatccc
     4441 taaccacacc acacgcgaac gcagccattg accggcgtca tcaagcgtga gtgtgaaacc
     4501 aggttgacga atccgtccgg ctaaaagaga aagcgccttc tctttctctg cgctcacttc
     4561 gcctaaaaat gccagagtca gatgcaaatt atcggcggcg accggacgtc ccgcctcagg
     4621 tgggaagtgt gtggcgcgcc agtggataat ctgttcgcgg atttctgcag gtaagtcgat
     4681 agcaaagaac agccgttgcg gttcagacat gtgaggcact cggttatgaa ttaccgcgat
     4741 gctacaatgt ggcgcgaaga atgttaaccc tctggagcgt tttgtgtcgt cgttgcccgt
     4801 tgctgccgtc ttacctgagt tacttgctgc cctcgatggt gcatcgcagg tgttattaag
     4861 tgcgccgacc ggggccggga aatcaacctg gctgccgctg caactgctgg cgcatcccgg
     4921 cattaacggg aaaattatcc tgctggagcc gcgtcgtctg gcggcgcgta acgttgcgca
     4981 acgactggcg gagctgctta acgaaaagcc aggcgatacc gttggctacc ggatgcgtgc
     5041 gcaaaactgc gtcgggccga atacccgcct ggaagtggtt accgaaggcg tgctgacgca
     5101 gatgatccag cgtgacccgg aactgagcgg tgttggattg gtgatccttg atgaatttca
     5161 tgagcgcagc ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc aacaaggtct
     5221 gcgtgatgac cttaaactgc tgattatgtc ggctacgctg gacaacgacc gcttgcagca
     5281 aatgctgcca gaagcgcccg tcgtcatctc agaagggcgc tcgtttccag ttgaacgccg
     5341 ttatttgccg ctgcccgctc atcagcgttt tgacgaagcc gtcgcggttg ccaccgccga
     5401 aatgctgcgt caggaaagtg gatcattact gttattttta cctggcgtcg gagaaattca
     5461 gcgtgtgcag gaacaactga cttcacgcat cggcagtgat gtgttgctct gcccgctgta
     5521 tggcgcgttg tcgctgaacg atcagcgaaa agcgatcctc ccggcaccgc aagggatgcg
     5581 taaagtggtg ctggcgacca atattgctga aaccagttta accatcgaag gtattcgtct
     5641 ggtggtggat tgtgcccagg agcgtgtggc gcgttttgat ccgcgtacag ggcttacgcg
     5701 gctggttact caacgtgtta gtcaggcgtc gatgacgcaa cgtgccgggc gtgccgggcg
     5761 tctggagccg ggtatctgcc tgcatttaat cgccaaagaa caagcagaac gcgctgccgc
     5821 gcaaagtgaa ccggaaatat tacaaagcga tctttcctgt ttgctgatgg aattactgca
     5881 atggggatgc agcgatccgg cgcagatgag ctggctggat caaccgccaa cggtgaatct
     5941 actggccgcg aaacgcctgt tacggatgct gggggcgctg gacggtgaac ggcttagtgc
     6001 gcaagggcaa aaaatggcag cgctgggtaa cgatccgcgt ttagcggcaa tgctggttag
     6061 cgcgaagaac gacgacgaag ctgctaccgc ggcaaaaatt gccgccattc tcgaagagcc
     6121 gccacggatg ggcaatagtg acctgggcgt ggcgtttttg cgcaatcaac ccgcctggca
     6181 gcaacgtagt cagcaactgt taaaacgttt aaacgtacgc ggcggtgagg cagacagttc
     6241 gcttatcgcg ccgcgacttg ccggagcgta tgccgatcgt attgctcgcc gccgtgggca
     6301 agagggacgc tatcaactgg cgaacggcat gggggcgatg ctcgatgccg acgatgcgct
     6361 tagccgccac gaatggttga tcgcgccgtt attattgcag ggcagttctt cgccggatgc
     6421 gcggatttta ctggcgctgc cggtcgatat tgatgagtta gtacaacgct gcccgcagct
     6481 ggtacagcag tctgacaccg tggagtggga tgacgcgcaa ggtacgctga aagcctggcg
     6541 tcggctgcaa atcggtcagt tgacggtaaa agtgcaaccg ctggcgaaac cctcggaaga
     6601 cgagttgcat caggcgatgc ttaacggcat tcgtgataaa ggtttaagcg tgctcaactg
     6661 gacggcggaa gcggaacagc tacgcttgcg tttgttatgt gccgcaaagt ggttgccgga
     6721 atatgactgg ccagcggttg atgatgaaag tttgttggcg acgctggaaa cgtggctact
     6781 gccgcatatg acaggcgtac attcgctacg cggcctgaaa tcactcgata tttatcaggc
     6841 actgcgtgga ttacttgatt gggtaatgca gcaacgtctg gatagtgaat tgcctgcgca
     6901 ttacactgtg ccgacgggaa gccggatcgc cattcgttac catgaagata acccgcccgc
     6961 gctggcggtg agaatgcagg agatgtttgg tgaggcctct aatccgacta tcgcccaggg
     7021 gcgcgtaccg ttggtgctgg agttgctttc ccctgcccaa aggccgctgc aaatcacgcg
     7081 tgatttgagc gccttctgga aaggagcgta ccgtgaggtg caaaaagaga tgaaagggcg
     7141 ttatcccaaa catgtctggc cggacgaccc ggcaaacacc gcaccgacgc gacgaacgaa
     7201 aaagtattcg tgaagaccaa agcaaatttg agagatatct tcttctgtct tgcaacagaa
     7261 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7321 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7381 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7441 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7501 gctatggctg ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7561 ttatctcgat caaaaaattc gcagccgtat tgatggcaag gtctggcaat tgcccgcggc
     7621 agtttatggt cgaatggtca accttgagcc agacatgacc atcagcaaga acgagatggt
     7681 gaagctgctg gaggcgaccc agtatcgcca ggtgtcgaaa atgacccgtc ctggtgagtt
     7741 taccgtacag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagcaa
     7801 agaagggcag gtgcgcgcgc gtctgacctt tgatggcgat catctggcga cgatagtcaa
     7861 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgcctga tcaccatgat
     7921 ctcttcgcca aacggtgagc agcgtctgtt tgtgccgcgc agtggtttcc cggatttgct
     7981 ggtggatact ttgctggcga cagaagaccg tcatttctac gagcatgatg ggatcagtct
     8041 ctactcaatc ggacgtgcgg tgctggcaaa cctgactgcc ggacgcacgg tgcagggggc
     8101 gagtacgctg acgcaacagt tggttaaaaa cctgtttctc tccagcgagc gttcttactg
     8161 gcgtaaagcg aacgaagcct acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8221 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8281 cggcttcccg ctggcgagct tgtattactt tggtcgcccg gtagaagaat tgagcctcga
     8341 tcagcaggcg ctgttggtgg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8401 cccgaaactg gcgctggagc gacgtaacct ggtactacgt ttgctgcaac agcaacaaat
     8461 tattgatcaa gaactctatg acatgttgag tgcacgtccg ctgggtgttc agccgcgcgg
     8521 tggggtgatc tctcctcagc cagcctttat gcagctggtg cgtcaggagc tgcaggcgaa
     8581 attaggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8641 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     8701 taagttgagc gatcttgaaa ccgcgattgt ggttgttgac cgctttagcg gtgaagttcg
     8761 tgcgatggtc ggtggctctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     8821 tcgttcgatt ggttcccttg caaaaccagc gacttatcta acggccttaa gccagccgaa
     8881 aatctatcgt ttgaatacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     8941 ccaggtctgg tcaccgcaga acgatgatcg tcgttatagc gaaagtggca gggtgatgct
     9001 ggtagatgcg ttgacccgtt cgatgaacgt gccaacggtg aatctgggca tggcgttggg
     9061 gctgcctgcg gttacggata cctggatcaa actgggtgtg ccgaaagatc aactgaatcc
     9121 agttccagca atgttgttgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcctt
     9181 ccagactatc gccagcggtg gtaatcgtgc tccgctttcc gcgctacgtt cagtcatcgc
     9241 agaagacggc aaggtgttgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9301 ggcggcgtat ctgacactat ggaccatgca gcaggttgta caacgcggta cgggacgtca
     9361 gcttggggca aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9421 cgtagatacc tggtttgcgg gcattgatgg gagcacggtg accatcacct gggtcggccg
     9481 tgataacaac cagccgacca aactgtatgg tgccagcggg gcaatgtcga tttatcagcg
     9541 ttatctggct aaccagacgc caacaccgct gaatcttgtt ccgccggaag atattgcaga
     9601 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggtatgcgtg tcttgccagt
     9661 ctggaccagc gatccgcaat cgctgtgcca gcagagtgag atgcagcagc aaccgtcagg
     9721 caatccgttt gatcagtctt ctcagccgca gcaacagcaa cctgctcaac aggagcaaaa
     9781 agacagcgac ggcgtggctg gctggatcaa ggagatgttt ggtagtaatt aatatcgtaa
     9841 tcgct
//
LOCUS       DACADA010000159         9796 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.161, whole genome shotgun sequence.
ACCESSION   DACADA010000159 DACADA010000000
VERSION     DACADA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.161"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            181..1158
                     /gene="csiD"
                     /locus_tag="ISB17_003931"
     CDS             181..1158
                     /gene="csiD"
                     /locus_tag="ISB17_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAP1981811.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1178..2443
                     /gene="lhgO"
                     /locus_tag="ISB17_003932"
     CDS             1178..2443
                     /gene="lhgO"
                     /locus_tag="ISB17_003932"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAP1981812.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            2469..3917
                     /gene="gabD"
                     /locus_tag="ISB17_003933"
     CDS             2469..3917
                     /gene="gabD"
                     /locus_tag="ISB17_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP1981813.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3931..5211
                     /gene="gabT"
                     /locus_tag="ISB17_003934"
     CDS             3931..5211
                     /gene="gabT"
                     /locus_tag="ISB17_003934"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAP1981814.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5448..6848
                     /gene="gabP"
                     /locus_tag="ISB17_003935"
     CDS             5448..6848
                     /gene="gabP"
                     /locus_tag="ISB17_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAP1981815.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6869..7531
                     /gene="csiR"
                     /locus_tag="ISB17_003936"
     CDS             6869..7531
                     /gene="csiR"
                     /locus_tag="ISB17_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAP1981816.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7532..7981)
                     /gene="kbp"
                     /locus_tag="ISB17_003937"
     CDS             complement(7532..7981)
                     /gene="kbp"
                     /locus_tag="ISB17_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAP1981817.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8065..8223)
                     /locus_tag="ISB17_003938"
     CDS             complement(8065..8223)
                     /locus_tag="ISB17_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAP1981818.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8406..8705
                     /locus_tag="ISB17_003939"
     CDS             8406..8705
                     /locus_tag="ISB17_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1981819.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8715..9239
                     /gene="ygaP"
                     /locus_tag="ISB17_003940"
     CDS             8715..9239
                     /gene="ygaP"
                     /locus_tag="ISB17_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAP1981820.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9286..9690)
                     /gene="stpA"
                     /locus_tag="ISB17_003941"
     CDS             complement(9286..9690)
                     /gene="stpA"
                     /locus_tag="ISB17_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAP1981821.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2251 a         2533 c         2662 g         2350 t
ORIGIN      
        1 tcctttcaac ccaattctta tccctcacat tacttgttat taatttgctg tttttgatca
       61 caataagaaa acaatatgta acttttatgc gcatttttca gaaatgtaga tatttttaga
      121 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
      181 atgaatgcac tgaccgccgt acaaaataac gctgtcgatt caggccagga ctatcgcgga
      241 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
      301 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
      361 tcgtttctgc gttttcaggt aggcaaaatt ctcgacgatc tgtgtgcgaa tcagctgcaa
      421 ccgctgctgt tgaagaccct gttaaaccgc gctgaaggtg cgctgttgat caatgcggtg
      481 ggtatcgatg atgtcgcgca ggcggatgag atggtgaagc tggcgacggc ggtggcgcat
      541 ctgattggcc gttccaattt cgatgccatg agcggtcagt attacgcgcg tttcgtggtg
      601 aaaaatgttg ataactcaga cagctatctt cgtcagccgc accgcgtaat ggagctgcac
      661 aacgacggca cttacgtcga agagatcact gattacgtgc tgatgatgaa aatcgacgag
      721 caaaacatac agggcggtaa ctcgctgctg ctgcatctcg atgactggga acatctggac
      781 ctctttttcc gccatccgct ggcgcgccgt ccgatgcgct ttgccgcgcc gccgagcaaa
      841 aacgtcagca aagatgtctt ccatccggtg ttcgatgtcg atcagcaggg ccgcccagtg
      901 atgcgctata tcgaccagtt tgtccagccg aaagacttcg aagaaggcgt gtggttgagc
      961 gagctttcgg acgccattga aaccagtaaa ggcactcttt ctgtacccgt tcccgttggc
     1021 aaattcctgt tgattaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac
     1081 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac
     1141 taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     1201 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatatctatc ctgacgcccg
     1261 cattgcgttg ctggaaaaag agtccggccc tgcctgtcac cagacgggcc acaacagcgg
     1321 cgtgatccat gccggagtct attacacgcc cggtagcctg aagacgcagt tttgcctggc
     1381 ggggaaccgc gccactaaag tcttttgcga tcaaaacggc attcgctacg acaactgcgg
     1441 caagatgctg gtcgccacgt ctgaactcga aatggaacgg atgaatgcgt tatggaaacg
     1501 cacggcggca aacgggatcg agcgcgagtg gttaaacgcc gaggagctgc gcgagcgtga
     1561 accgaatatc accgggcttg gcggcatttt tgtgccgtcc agcggcattg tcagctaccg
     1621 cgaagtgacg gcggcgatgg caaaaatctt ccaggccaga ggtggtgaga ttatttataa
     1681 cgccgaagtc agcgccctca gtgagcataa aaacggcgtg gtgatacgta cccgtcaggg
     1741 cggtgaatat gaagcatcga cgctgattag ttgttccggg ctgatggctg accggctggt
     1801 gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgcggcg agtatttccg
     1861 ccttgcgcca gagcataacc agattgttaa ccacctgatt taccccattc ccgacccggc
     1921 aatgccgttt ttgggcgtgc atctcacccg catgatcgac ggcagcgtga ccgtcgggcc
     1981 aaacgcggtg ctggctttca aacgcgaagg ctaccgcaag cgcgacttct catttagtga
     2041 cacgctggag attttaggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     2101 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
     2161 gtattgtccc cggctttcgt taagcgatct ccagccctgg ccccccggtg tgcgggcgca
     2221 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
     2281 gatccacacc tgtaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     2341 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     2401 agcggcacgt agtgtggatg ccttacacgc cgcatttaat taataacctt tgaaaacagg
     2461 atgtagcgat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
     2521 aatggctgga cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca
     2581 agctgggtag cgtgccgaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
     2641 gccgcgccct gcccgcctgg cgtgcgctca ctgccaaaga acgcgccaac attctgcgca
     2701 actggttcaa tttaatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     2761 agggcaaacc actggctgaa gcgaaaggcg aaatcagcta cgccgcctca tttattgagt
     2821 ggtttgctga agaaggcaaa cgtatttatg gcgacaccat tcccggtcat caggccgata
     2881 aacgcctgat tgtgatcaag cagccgattg gcgtcaccgc ggctatcacg ccatggaact
     2941 tcccggcggc gatgattacc cgtaaagctg gtccggcgct ggcggctggc tgcaccatgg
     3001 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcagaa ctggcgattc
     3061 gcgcgggcgt tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtcggta
     3121 acgaactgac cagcaacccg ctggtgcgca agctgtcgtt taccggctcg accgaaattg
     3181 gccgtcagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     3241 gcaacgcacc gtttattgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     3301 tggcctcgaa attccgtaac gccgggcaaa cctgcgtctg cgccaaccgc ctgtatgtgc
     3361 aggacagcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     3421 tcggcgacgg gctggagaaa ggcgtcacca tcgggccgtt gatcgatgaa aaagcggtag
     3481 caaaagtgga agagcatatt gctgatgcgc tggagaaagg cgcgcgcgtg gtttgcggcg
     3541 gtaaagcaca tgaacgcggc ggtaacttct tccagccgac cattctggta gacgttccgg
     3601 ctaacgccaa agtgtcgaaa gaagagacgt ttggccccct cgccccgctg ttccgtttta
     3661 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga atttggactt gcagcctatt
     3721 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcactggag tacggcatca
     3781 tcggcatcaa caccggcatt atttccaatg aagtggcccc gttcggcggc attaaagcct
     3841 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     3901 tgtgcatcgg tctttaactg gagaatgcga atgatcagca ataaagagtt aatgcagcgc
     3961 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     4021 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     4081 gcggtgctca ataccgggca cctgcatccg aaagtggttg cggcggtgga agcgcaattg
     4141 aaaaaactgt cgcacacctg cttccaggtg ctggcctacg agccgtatct ggagctgtgc
     4201 gagattatga atcagaaggt gccgggcgat tttgccaaga aaactctgct ggttacgacc
     4261 ggttccgaag cggtggaaaa tgcggtgaaa atcgcccgcg ccgccactaa acgtagcggt
     4321 actatcgcct ttagcggtgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     4381 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     4441 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     4501 ttcaaaaatg acgccgcgcc ggaagatatc gccgccatcg tgattgagcc cgttcagggc
     4561 gaaggcggtt tctacgccgc gacgccagcc tttatgcagc gtttacgcgc gctgtgtgac
     4621 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcagggcg taccggcacg
     4681 ctgtttgcga tggagcaaat gggcgtggca ccagatctca ccacctttgc gaaatcgatc
     4741 gcaggcggtt tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga cgccgtcgca
     4801 ccgggcggac tgggtggcac ctatgccggt aacccgattg cctgcgtagc ggcgctggaa
     4861 gtgttgaagg tgttcgagca ggaaaatctg ctgcaaaaag ccaacgttct ggggcagaag
     4921 ctgaaagacg gattgctggc gatagccgaa aaacacccgg agatcggcga cgtacgcggt
     4981 ctgggggcga tgatcgccat cgagctgttt gaagatggcg atcacagcaa accggacgca
     5041 aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gtctgattct tctctcctgc
     5101 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     5161 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagctgta gcaccgcacc
     5221 atgccggagg cgacgctttg cggcttgtcc ggcctacgag aatccaggtc ggataaggcg
     5281 tttacgccgc atccgacaat ctgtacgtga acaggaataa atctatgtcg gccggataag
     5341 gcggagccgc tctccggcaa aaagaaccaa taacaattaa acgcgtgacc cgacgcggga
     5401 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     5461 cgcaaccaca tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg
     5521 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     5581 cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt atgcggatgt
     5641 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     5701 ccattggccg ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     5761 ttatcccact tgaagccaac atcgccgcca tgatcctgca ctcatgggtt ccaggcattc
     5821 ccatctggtt attttccctc gtcattaccc tcgccttaac cggcagtaat ttattaagcg
     5881 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg taaagtcatc gctatcctgg
     5941 cctttatttt ccttggtgcc gtcgcaatta gcggttttta cccgtatgcc gacgtgagcg
     6001 ggatttcaag attgtgggat agcggcggct ttatgcccaa cggcttcggt gcggtattaa
     6061 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg
     6121 cggaatccga cacgccggaa aaacatattg ttcgcgccac caactcggtt atctggcgta
     6181 tctctatttt ctatttgtgt tctatttttg tcgttgttgc attaatcccg tggaatatgc
     6241 cagggctgaa agccgttggt tcttatcgct cggttctgga attgctcaat attcctcatg
     6301 cgaaattaat catggactgc gtgatattac tttccgtaac cagttgcctg aactcggcgc
     6361 tgtataccgc ttccaggatg ctctactcct taagccgtcg cggtgatgct cccgcggtaa
     6421 tgggtaaaat caaccgcagt aaaaccccgt acgtagcggt gttactctcc accagcgcgg
     6481 cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtgtttaaa tttctgatcg
     6541 acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca caattgcgga
     6601 tgcgcaaaat tctgcgagca gaaggaagcg aaattcgcct gcggatgtgg ctttatccgt
     6661 ggctcacctg gctggtcatc ggctttatta cctttgtgtt ggtagtgatg ctattccgcc
     6721 cggcgcaaca gttagaagtg atctccaccg gcttattagc gattgggatt atctgtaccg
     6781 tgccaattat ggctcgctgg aaaaagctga taatgtggca aaaaacaccc attcataata
     6841 cgcgctgata atgatcagga gtcacaccat gaccattacc tctctggatg gctatcgctg
     6901 gctgaagaac gatattattc gcggcaattt tcagccggat gaaaaattac ggatgagttt
     6961 gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcacttt cgcaactggt
     7021 ggcagaacga ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga
     7081 gcaggagctg ctcgatattt tcgacgcccg cgccaatatg gaagcgatgc tggtgagtct
     7141 ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct
     7201 cagtaagctt gaggcctgtg acgccagtga gaaaatgctt gatgagtggg atctgcgtca
     7261 tcaggcgttt catactgcaa ttgtggcggg ctgtggttct tactatttgc tgcaaatgcg
     7321 tgaacggttg tttgatctgg cagcgcgtta tcgatttatc tggctgcggc gaacagtgct
     7381 ttcggtggaa atgctggaag ataaacacga tcagcaccag accctgactg cggcggtact
     7441 ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc
     7501 cattatccag caggcgatgg ctggcaatta actactcttc aggaatacgc aacacttgcc
     7561 ccgggtaaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
     7621 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
     7681 cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca tcgacactgg
     7741 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgacttaagc
     7801 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag
     7861 gtataccggt tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
     7921 gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
     7981 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
     8041 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
     8101 accggggata tagccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
     8161 tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc
     8221 catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc
     8281 gtagtaattt tagcggagcc tgccagtttc agcgtacgct tttactctca tttacgctgc
     8341 tgatgctggt atatgttgca cacttagttt agagtttact taatttagaa aatacttaaa
     8401 caactatgac tgaactcgca caattacagg ccagtgccga acaggcagcg gccttattga
     8461 aatcaatgag ccaccctaaa cgattgctga ttctgtgtat gcttagcggt tcccccggca
     8521 ccagcgcggg agagctgacg cgcattaccg gactgagtgc ctctgcgaca tcacagcatc
     8581 tcgctcgtat gcggggcgaa gggcttatcg actgccaacg ggatgcccaa cgcattctat
     8641 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
     8701 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
     8761 cacgcggcgc aaagttaatc gatattcgtg atgctgatga atatcttcgt gaacatattc
     8821 ctgaagcaga tctggctcca ttatccgtac tggaacagtc aggtcttccg cctaaattac
     8881 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgctgata
     8941 aattagcagc gattgccgcc cccgcagaaa tctttttact cgaagatggg attgatggct
     9001 ggaaaaaagc aggattgcca gtagcggtaa ataaatccca acccttaccg ttaatgcgcc
     9061 aggtgcaaat cgccgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
     9121 atagcgtttt cttcttatta agcggctttg ttggtgctgg gttactgctt gcaggaatta
     9181 gtggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag
     9241 cagcgacgcc ggacgcgcca tagcagcgac atccggcctc agtaattaga tcaggaaatc
     9301 gtcgagagat ttaccttctg ccagcgcctg agcgattggc ttcggtgtac ggccctgacc
     9361 ggtccaggtt ttagtttcac cgttaacatc gatgaattta tatttcgccg gacgcggctg
     9421 gcgtttttta ccagcgcgtg gtgccgcagc agagctatta cccaataact cttccgggtt
     9481 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctggc gctctgccag
     9541 ttcacgctgc tgttgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
     9601 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
     9661 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa
     9721 aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct
     9781 atttacagtc ctgcac
//
LOCUS       DACADA010000160         9749 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.162, whole genome shotgun sequence.
ACCESSION   DACADA010000160 DACADA010000000
VERSION     DACADA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.162"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            139..1296
                     /gene="acrE"
                     /locus_tag="ISB17_003942"
     CDS             139..1296
                     /gene="acrE"
                     /locus_tag="ISB17_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAP1981822.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1308..4412
                     /gene="acrF"
                     /locus_tag="ISB17_003943"
     CDS             1308..4412
                     /gene="acrF"
                     /locus_tag="ISB17_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAP1981823.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4665..4886
                     /locus_tag="ISB17_003944"
     CDS             4665..4886
                     /locus_tag="ISB17_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981824.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5317..6342
                     /locus_tag="ISB17_003945"
     CDS             5317..6342
                     /locus_tag="ISB17_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1981825.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6410..7591
                     /locus_tag="ISB17_003946"
     CDS             6410..7591
                     /locus_tag="ISB17_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP1981826.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7601..8704
                     /locus_tag="ISB17_003947"
     CDS             7601..8704
                     /locus_tag="ISB17_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP1981827.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8712..9470
                     /locus_tag="ISB17_003948"
     CDS             8712..9470
                     /locus_tag="ISB17_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP1981828.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(9533..9648)
                     /gene="rrf"
                     /locus_tag="ISB17_003949"
     rRNA            complement(9533..9648)
                     /gene="rrf"
                     /locus_tag="ISB17_003949"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2320 a         2336 c         2509 g         2584 t
ORIGIN      
        1 gagttgccag tattcataac tattccttac atcgacgaat gataatttgt aggatagcga
       61 actgtatttt tctttctgcg agttaacgca ttgccttttt gggcaaataa cgcgcttttg
      121 gttttttgag gaatagtaat gacgaaacat gccaggtttt tcctcctgcc ctcctttatt
      181 ctgatctccg cggctttaat cgccggttgt aacgataagg gagaagagaa agctcacgtc
      241 ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc cgttagaagt taagactgaa
      301 ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc gcccacaggt tagcgggatc
      361 gtactgaatc gcaatttcac tgaaggcagc gatgtgcaag caggccagtc cctgtaccag
      421 atcgatcccg cgacctatca ggcaagttat gacagcgcga aaggcgaact ggcgaaaagt
      481 gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt acgttccgct cgtgggtacg
      541 aaatacatca gtcagcagga gtacgaccag gccattgctg atgctcgcca ggccgatgcc
      601 gccgtgattg ccgcaaaagc cacagtcgaa agcgctcgca tcaatctcgc ttataccaaa
      661 gtgactgcgc caattagcgg acgtatcggc aaatcgaccg tgaccgaagg cgctcttgtc
      721 actaatgggc aaacgactga actggcgact gtccagcagc tcgatcctat ctacgttgat
      781 gtgacccaat ccagcaacga ttttatgagg ctgaagcaat ccgtagagca aggaaatttg
      841 cataaggaaa acgccaccag caacgtagag ttggtcatgg agaacggtca aacctatccc
      901 ctgaaaggta cgctgcaatt ctccgatgtg accgttgatg aaagcaccgg ctccataacc
      961 ctacgtgctg tcttccctaa cccgcaacat acgcttttgc cgggtatgtt tgtgcgcgca
     1021 cggattgatg aaggcgtcca acctgacgcc attcttatcc cgcaacaagg cgttagccgc
     1081 acaccacgtg gtgatgcaac cgtgctgatt gttaacaata aaagtcaggt tgaagcgcgc
     1141 cctgtcgttg ccagtcaggc gattggcgat aaatggttga ttagcgaagg tctgaaatct
     1201 ggcgatcaag tcattgtcag cggcctgcaa aaagcgcgtc cgggagagca ggttaaagcc
     1261 actaccgata cccccgcaga tactgcatcg aagtaaggca atctgacatg gcaaactttt
     1321 ttattcgacg accgatattt gcatgggtgc tggccattat tctgatgatg gcgggcgcac
     1381 tggcgatcct acaattgccc gtcgcccagt atccaaccat tgcgccgcct gcggtttctg
     1441 tttcggcaaa ctatccgggt gctgatgcgc agaccgtgca ggatacagtg acgcaggtta
     1501 tcgaacagaa tatgaacggt atcgataacc tgatgtatat gtcctccacc agcgattccg
     1561 ccggtagcgt gacaattacc cttaccttcc agtccgggac cgatcctgat atcgcgcaag
     1621 tacaggtgca gaacaaactc cagctcgcca cgccgttgct gccgcaggag gttcagcagc
     1681 aggggatcag tgttgaaaag tccagtagca gctatttgat ggtggcgggc tttgtctctg
     1741 ataaccctgg caccacacag gacgatatct cggactatgt ggcctctaac gttaaagata
     1801 cgcttagccg tctgaatggc gtcggtgacg tacagctttt cggcgcacag tatgcgatgc
     1861 gtatctggct ggacgccgat ctgctaaaca aatataaact gacaccggtt gatgtgatta
     1921 accagttgaa ggtacagaac gatcagatcg ctgccggaca gttgggcgga acgccagcgt
     1981 taccagggca acagttgaac gcctcgatta ttgctcagac gcggttaaaa aatccggaag
     2041 aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc ggtggtgcgt ctgaaagatg
     2101 tcgcacgggt tgaacttggc ggtgaaaact ataacgttat cgctcgcatc aacggaaaac
     2161 cggcggcggg cctggggatt aagctggcaa ccggcgcgaa tgctctcgat accgcgaaag
     2221 ccattaaggc aaaactggcg gaattacagc cattcttccc gcagggaatg aaggttcttt
     2281 acccttatga caccacgcct ttcgtccagc tttctatcca ggaagtggtg aaaacgctgt
     2341 tcgaagccat tatgctggtg ttcctggtga tgtatctgtt cttgcagaac atgcgggcaa
     2401 cattgatacc caccattgct gtacctgtcg tgttgttagg gacatttgcc atactcgccg
     2461 cttttggtta ctccatcaat acgctaacga tgtttgggat ggtacttgcc atcggacttc
     2521 ttgttgatga tgcgatagtc gttgtggaga acgtcgagcg cgtaatgatg gaggataaac
     2581 tcccccccag agaagcgacg gaaaaatcga tgtcgcaaat ccagggcgcg ctggtaggta
     2641 tcgcgatggt actgtcggcg gtatttattc cgatggcatt cttcggtggc tcaacagggg
     2701 caatttatcg ccagttctct atcactattg tttcagcaat ggcgctttca gtactggttg
     2761 cgctgattct cacccccgcg ttatgtgcaa cattgctgaa accagtctct gccgagcatc
     2821 acgaaaataa gggaggattc ttcggctggt ttaacaccac tttcgatcat agcgttaatc
     2881 actacaccaa tagcgttggc aaaatcctcg gttccacagg gcgatattta ctgatctatg
     2941 cgctgattgt tgcaggaatg gtggtgttgt ttttacgtct tccgtcttcc ttcttacctg
     3001 aagaggatca gggtgtcttt ctgaccatga ttcagttacc cgctggcgcg acgcaagagc
     3061 ggacgcaaaa agtgttggat caagtgacgg attactatct gaagaacgag aaagcgaacg
     3121 ttgaaagtgt ctttacagtt aacggcttta gcttcagcgg ccaggcacaa aacgccggta
     3181 tggccttcgt cagtctgaaa ccgtgggaag agcgtagtgg tgacgaaaac agtgcggaag
     3241 cagtaatcca tcgtgccaaa atggaattgg gcaagatccg cgacggtttt gtcattccat
     3301 ttaatatgcc agccattgtt gaactaggca cggcaacggg tttcgacttt gagttaattg
     3361 atcaggctgg gctgggtcac gatgccctga cccaggcccg taaccagttg cttggcatgg
     3421 cggcgcaaca tcctgccagc ttagtcagcg tgcgccctaa tggcctggaa gacaccgcgc
     3481 agtttaaact ggaagttgac caggaaaagg cgcaggcatt aggtgtttca ctttctgaca
     3541 tcaatcagac catttcaacg gcgttgggtg ggacttacgt taacgacttc atcgaccgtg
     3601 gtcgcgtgaa aaaggtgtat gttcaggcgg atgccaaatt ccgtatgctg ccagaagatg
     3661 tcgataaact ttatgtccgc agcgccaacg gcgaaatggt gccattctct gcctttacca
     3721 cttcacattg ggtgtatggc tctccgcgac tggaacgcta caacggtctg ccgtcaatgg
     3781 agattcaggg ggaagccgcg ccaggaacca gttccggcga tgctatggcg ttgatggaaa
     3841 accttgcgtc aaaattaccg gcgggcattg gttatgactg gacgggtatg tcatatcagg
     3901 aacgtttatc gggaaaccag gctcccgctc tggtagcaat ttcctttgtg gttgttttcc
     3961 tgtgccttgc tgcactctat gaaagctggt caattcctgt ctcggttatg ttggtcgtgc
     4021 cgttagggat tgtcggcgtg ctactggcgg caacactctt taatcaaaaa aatgacgtct
     4081 actttatggt gggcttgcta acgacaattg gcttgtcggc caaaaacgct attttgattg
     4141 ttgagttcgc taaagatctc atggagaaag agggtaaagg tgttgttgaa gcgacactaa
     4201 tggcagtacg tatgcgcctg cgtcctatcc tgatgacctc tctcgccttt attctcggcg
     4261 tattaccgct agctatcagt aacggtgccg gcagtggcgc gcagaacgca gtgggtatcg
     4321 gggtaatggg cgggatggtt tctgcaacgt tgctggcaat cttcttcgtg ccggtgttct
     4381 ttgtggtgat ccgccgttgc tttaaaggat aaatcagaaa cataaaggca ctttcgggtg
     4441 cctttattat ttccagtgaa acccataaaa attaataaga tattcctcta ctcacttttt
     4501 aaaaactttc tatagttccc gctcccttca ctatttttac aattcacata atttgtcatt
     4561 attcattccg caagatttat aacctccagt gataaaatgg cattcagctc gttaataaga
     4621 gagttaactt attaagcgtt agcgtttatt actgaggtaa caccatgaaa agattaattc
     4681 ctgtggcatt gctcaccgca ttgctggcgg gctgtgctca cgattcgccc tgcgttccgg
     4741 tttatgacga tcagggtcga ctggttcaca ctaatacctg tatgaaaggc acgactcagg
     4801 ataactggga aacggctggg gctattgccg gcggggcagc agctgttgct ggcctgacga
     4861 tggggatcat cgctctgtcg aaataactaa acagggcgcg gagctccgcg ctctttttaa
     4921 cgatacagta tctttccata agataaaata ttgctatatt ctgctgggta attccctgct
     4981 gatttagcat gttatatcac gttatttcgc tgctttcgaa ataacgcatc aatttaactt
     5041 tcacacttcc caccagaaat atactcacgc taacaatcat tattctgctt agtaaaaaac
     5101 gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt ttcgcaccat tacgaggcgc
     5161 tgtttttatt atttcctgtc tacactcagc ttattgcgac gcgaaatcgt gcgcaaaagc
     5221 tggcactact tttgcttata agaggatggc cacagacagg taaaagacgt ttcccaaacg
     5281 tcctataacg ataattttcg cctcacagga agcattatga aaaagatgat gatagccaca
     5341 ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg cgcatgctgg cgcgacgctt
     5401 gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca gtgatggatt acctgggttc
     5461 tcttatgccg atgctgacgg taagttttca ggtattgatg tagatgtttg tcgtggtgtt
     5521 gccgctgctg tattcggtga cgatacgaaa gtgaaatata ccccgctcac tgcaaaagaa
     5581 cgcttcaccg ctttacagtc aggggaggtg gatttgctct cccgtaatac gacctggact
     5641 tcatctcgcg atgccgggat gggaatggca tttactggcg tcacttatta cgacggcatt
     5701 ggcttcctga cgcacgataa agcggggcta aaaagcgcga aagaactgga tggcgctacc
     5761 gtctgtattc aggcggggac tgataccgaa ctcaacgtcg ccgattactt caaggcaaat
     5821 aatatgaagt acacgccagt aaccttcgat cgctcagacg aatcagcgaa ggctctggaa
     5881 tctggccgct gtgatactct ggcctcggat caatcacaac tgtatgccct acgcatcaaa
     5941 ttaagcaacc caactgaatg gattgtctta ccggaagtta tctctaaaga accacttggg
     6001 ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg tacgctggac gcttttcgcc
     6061 atgctgaatg ctgaagagat gggcatcaat tcccagaatg tcgatgaaaa agcggctaat
     6121 ccagcaacgc ctgatatggc acatctgctg ggtaaagaag gcgattacgg caaggatctg
     6181 aagctggata ataaatgggc ctataacatc atcaaacagg tgggtaacta ctcggaaatt
     6241 ttcgagcgta acgtaggttc agaaagcccg ctgaaaatta aacgtgggca aaataatctc
     6301 tggaataacg gcggtattca gtacgcgccg cccgtgcgtt aagtcggtgt gaggtaacga
     6361 gcgttgctac ggcggcgctc ttttcagcgt tatggttttc gaggtttcta tgccccatcg
     6421 ccgctcaacc gttaaaggct cactctcttt tgccaaccct acggttcgcg cctggttatt
     6481 ccagatcctt gccgttgttg ctgctgtcag cattgttggt tggttatttc acaacactgt
     6541 aacgaatctc agtaatcgtg gcataacatc aggatttgct tttcttgacc gcggggccgg
     6601 ttttggcatc gttcagcatt tgattgatta tcagcaaggc gacacttacg gacgcatttt
     6661 tattgtcggc ctactcaata cgctactggt ttctgcattg tgtattgtgt tcgcttctgt
     6721 tttgggcttc tttatcggtc tggcgagact ttcggataac tggctgctac gaaagctttc
     6781 cacaatttat attgagatct tccgtaatat tcccccgctg ctgcaaatct tcttctggta
     6841 ctttgccgtg ttgcgcaatt tgcccggacc acgacaggca gtgagcgcgt ttgacctggc
     6901 ctttttgagc aatcgtgggc tttatattcc gtcgccgcag ctgggagacg gatttattgc
     6961 gtttatcctg gctgttgtta tggctatagt cctttctgtt gggctattcc gctttaataa
     7021 aacataccag ataaagaccg gacaactgcg ccgcacctgg ccattcgccg ctgtgttgat
     7081 cattggtttg cctttactgg cgcaatggct ttttggcgca gcactacact gggatgtccc
     7141 agcccttcga ggctttaatt tccgcggcgg gatggtttta attcctgaac tggcagcctt
     7201 aacgctagca ctttcgattt atacctctgc atttatcgcc gagattatcc gcgcagggat
     7261 ccaggcagtg ccttatggtc aacatgaagc ggctcgatca ctgggattac ccaacccggt
     7321 tacgctacgc caggtcatta ttccccaggc attgcgggtg attattccac cgttaaccag
     7381 ccagtatctc aacatcgtca agaactcctc tcttgccgcc gctattggct atcccgatat
     7441 ggtttcgctg tttgctggca ccgtgctgaa ccaaacgggg caagccatcg agacgatagc
     7501 catgaccatg tcggtctatc tgattatcag cctgactatc tcgctgctga tgaatatcta
     7561 taaccgccgc atcgcgatcg ttgaacgcta aggagtcatg atgacaaaag tattgctgtc
     7621 tcatcccccg cgcccggcga gccataactc aagccgcgcg atggtgtggg tgcgaaaaaa
     7681 tctgttctcc agctggagca atagcctgct gactattggc tgcatatggt tgatgtggga
     7741 actgatccca ccgttgctga actgggcatt tttgcaggct aactgggttg gctcaacgcg
     7801 tgccgactgc acaaaagccg gtgcctgttg ggtcttcatc cacgaacgat ttggtcagtt
     7861 tatgtatgga ctttacccac acgaccaacg ctggcgaatt aacctggcat tactgattgg
     7921 ccttgtgtct atcgcaccaa tgttctggaa aatacttccg catcgcggtc gctatattgc
     7981 ggtctgggcg gtgatttacc cactggttgt ctggtggctg atgtatggtg ggtttcttgg
     8041 tcttgatcgg gttgaaaccc ggcaatgggg cgggttgacg ctaactttaa ttattgcatc
     8101 agttgggatt gcgggggcgc taccgtgggg gatcttactg gcgttaggtc gccgctccca
     8161 tatgccgatt gtgcgtatct tatcggtcat ttttatcgag ttctggcgcg gcgtaccgct
     8221 gattaccgta ttgtttatgt cttcggtcat gctgccgttg tttatggcag aaggcaccag
     8281 tatcgataaa ctgatccgcg cgctggttgg cgtgatcctg tttcagtcag catatgttgc
     8341 ggaagtcgtg cgaggcggat tacaggcgct gcctaaaggg caatatgaag cggcagagtc
     8401 gctggcattg ggttattgga aaactcaggg gcttgttatt ctgccccagg cgttgaagct
     8461 ggtaattcct gggctggtaa ataccatcat cgcactcttc aaagatacca gtctggtgat
     8521 catcatcggg ttgttcgatc ttttcagtag cgttcagcag gcaaccgttg atcccgcgtg
     8581 gttgggtatg tcgacggaag ggtatgtttt cgccgcgctg atctactgga tcttctgttt
     8641 cagcatgtca cgttatagcc aacatctgga aaaacgtttt aacaccgggc gtacaccgca
     8701 ttgaggacac tatgagccaa attttactgc aacctgctaa cgcgatgatt acgctggaaa
     8761 acgtcaataa atggtatgga caattccatg ttttgaaaaa tataaattta accgtgcaac
     8821 cgggcgaacg gatcgttcta tgtggccctt caggttccgg taaatcaaca accattcgtt
     8881 gtattaatca tctggaagaa catcaacagg gacggatcgt ggtagatggc atcgaactta
     8941 atgaagatat ccgcaatatt gagcgcgtca ggcaggaagt gggaatggtc tttcagcatt
     9001 tcaatctctt ccctcatctg accgtgttac agaactgtac cctggcaccg atttgggtac
     9061 gcaagatgcc aaagaaagag gctgaagctc tggcgatgca ttaccttgag cgggtgagaa
     9121 ttgccgaaca tgcccagaag tttcccggac agatttctgg tggtcagcag caacgtgttg
     9181 ccatagcacg ttcgctgtgt atgaagccga aaattatgtt gtttgatgag ccaacgtcgg
     9241 cgctcgatcc tgagatggtg aaagaggtgc tggatacgat gattgggctg gcgcagtcgg
     9301 gtatgacaat gttgtgtgta acacatgaga tggggtttgc acgaaccgtc gctgaccggg
     9361 tgatttttat ggatcgtggg gaaatagtgg agcaagctgc acctgatgaa ttttttgcgc
     9421 atcctaaatc agagcgtacg agggcatttt tatcgcaggt aatccattaa ttgaatatta
     9481 gttcgaaaag caaaaaggcc atcctttcgg atggcctttc gcctgatttg atgtctggca
     9541 gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc
     9601 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccagaca aattcttttc
     9661 taatctgccg aactttaacc taaaaagtgg tgctgatacc cagagtcgaa ctggggacct
     9721 cacccttacc aagggtgcgc tctaccaac
//
LOCUS       DACADA010000161         9745 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.163, whole genome shotgun sequence.
ACCESSION   DACADA010000161 DACADA010000000
VERSION     DACADA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.163"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            123..746
                     /gene="rcsA"
                     /locus_tag="ISB17_003950"
     CDS             123..746
                     /gene="rcsA"
                     /locus_tag="ISB17_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAP1981829.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(790..1032)
                     /gene="dsrB"
                     /locus_tag="ISB17_003951"
     CDS             complement(790..1032)
                     /gene="dsrB"
                     /locus_tag="ISB17_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAP1981830.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1141..1368
                     /gene="yodD"
                     /locus_tag="ISB17_003952"
     CDS             1141..1368
                     /gene="yodD"
                     /locus_tag="ISB17_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAP1981831.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1664..2479
                     /locus_tag="ISB17_003953"
     CDS             1664..2479
                     /locus_tag="ISB17_003953"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAP1981832.1"
                     /translation="MFSIQQPLLVFSDLDSTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWITATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2476..4170)
                     /gene="yedQ"
                     /locus_tag="ISB17_003954"
     CDS             complement(2476..4170)
                     /gene="yedQ"
                     /locus_tag="ISB17_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAP1981833.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLKNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4341..4523)
                     /locus_tag="ISB17_003955"
     CDS             complement(4341..4523)
                     /locus_tag="ISB17_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAP1981834.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4602..5519)
                     /locus_tag="ISB17_003956"
     CDS             complement(4602..5519)
                     /locus_tag="ISB17_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAP1981835.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5692..6612
                     /gene="yedA"
                     /locus_tag="ISB17_003957"
     CDS             5692..6612
                     /gene="yedA"
                     /locus_tag="ISB17_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAP1981836.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6601..7071)
                     /locus_tag="ISB17_003958"
     CDS             complement(6601..7071)
                     /locus_tag="ISB17_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAP1981837.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7052..8470)
                     /locus_tag="ISB17_003959"
     CDS             complement(7052..8470)
                     /locus_tag="ISB17_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP1981838.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8537..9232)
                     /locus_tag="ISB17_003960"
     CDS             complement(8537..9232)
                     /locus_tag="ISB17_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP1981839.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9272..9637)
                     /locus_tag="ISB17_003961"
     CDS             complement(9272..9637)
                     /locus_tag="ISB17_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1981840.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         2392 a         2580 c         2362 g         2411 t
ORIGIN      
        1 atagtaagtc cgcctgaaaa attgcgagag tggcgcatta ggtgacccat gttgttccgt
       61 ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg
      121 ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta accgggtatc
      181 tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg
      241 ccatagcttg tgattcacag cgcccttcag tggtgtttat taatgaggac tgtttcatcc
      301 acgatgcttc taacagtcag cgcatcaagc acatcattaa tcaacatccc aatacgttat
      361 ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa
      421 atttattgat cagctctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata
      481 ttctgaaaaa agatacaacg ataacctcgt ttttaaatat gccgacttta tcattgagcc
      541 gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt caaatctccg
      601 accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga
      661 tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta
      721 atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc
      781 tgaataagat tactctgctt tctcaacaaa gataccgtcc tgatgccctg cttcattaaa
      841 gaaccagatg ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa
      901 ctcctcaact gccagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac
      961 ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg
     1021 tttttctggc agcgccaggc gcgcgcgagt gctgattttc tcgacggtct atacttaaga
     1081 gatgccaggc ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg
     1141 atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat
     1201 gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat
     1261 tctgaacacg ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc
     1321 ttcgaactgg atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa
     1381 aaaatcccga ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa
     1441 ttcgttacac caggaaatct gatgtgttca tcaccttatc tgcaattttt ttcgctgaca
     1501 agaaaatatt cgcgctatga atgattaacg ctacttatga atagaaatgt gacgcgcgtc
     1561 accccttggt ggctctggtg tcaacgggat cgctcccgaa aaaatcatct ttgattattt
     1621 gtgctgtttt gagaatcatc ctggtaaggg gagtactgcg cccatgtttt caattcaaca
     1681 accactactg gtttttagcg atcttgatag caccctgctg gacagtcata gttatgactg
     1741 gcaaccggca gccccctggc tcagccgttt acacgaagcc aatattcccg tcattctctg
     1801 tagcagtaaa acttcagcgg aaatgctgta cttgcaaaaa atgttggggc tacaaggctt
     1861 accgctgatt gcagagaatg gcgcagtgat ccagcttgct gaacaatggc aggatataga
     1921 cggttttcca cgcatcatct caggtattag ccatggcgaa atctgccagg ttttaaatac
     1981 gctacgcgag aaagaacatt ttaaattcac gacttttgat gatgtcgacg atgcaaccat
     2041 tgccgaatgg acgggattaa gccgtagcca ggcggcgctg acgcagctgc atgaggcgtc
     2101 ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttatcg ctcgtctgaa
     2161 cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg cacgtactgg atgcctctgc
     2221 cggaaaagat caggctgcca actggattac cgcgacctat caacaattgt caggcagacg
     2281 cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cctttactgg aggtaatgga
     2341 ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtc catctgcatg atgaggatcc
     2401 ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag ggctggacca
     2461 ttttttctcc gcccgttaag cgttatcgct cgcgaatacc cgattacgcc cggcctgttt
     2521 tgcgaggtaa agccgacggt cggccaatga ctggagttgt tcaaaatcat aatcaccggt
     2581 ttcctcgctg ctacttaccc ccagcgaggc actgatgcgt atcgttgtac tcttggcgat
     2641 cagcatctct ttttcattta acttcaggcg aatacgttct gcgacctccg cagcctgcgt
     2701 cagattcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc cggcaacgtc
     2761 ctgcgcacgc aaggaactgc taattaatcc ggcagcatga gaaagaacac ggtcgcccgc
     2821 ctgatgacca aagcggtcat taatcgcttt aaaatggtca agatcgacct gaatgacaga
     2881 aaaaggatgt tggtgcgtct gacacaattt agcgagcgga cgggcttttt cgaacaatgc
     2941 gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc tgccactgca acgagctttg
     3001 cagaacatac atgttgctga ccatccggcg aatcacatac caggagatga gtaacatggt
     3061 ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat cgccgcgcac
     3121 gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg aaatggtcca gacgttccca
     3181 gctaacatag cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca ttgcctgcgc
     3241 cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag gatttgaaga
     3301 ggtcaaaaat ctcagcttac tgtcatagag ctgatactca ccatcgaggt ttttatcgat
     3361 ggcgtttttt aaaaattgct gcatagtacg cacgggaata ctcatcccca gcacgccata
     3421 ccagtagtta ttactgtcta ccggaacact gacggtaacc tgcggttcag tattgctggc
     3481 gtgttccggt tgcgaagtga accagcgtac cgcgcggtga cgattttctc gttgtgaatg
     3541 gccgataaac cagggttggg tgacatagcc gtaataacgc gttggtacat tgcgcgtaaa
     3601 caaggtcggc agcgtcgaaa cgtaaaatcc agcacgcgag acatacaccg cctgttcaac
     3661 catcgacgag gagttgtgcg ccagtcgcag caagtaacca acttccagtg cagcggtaat
     3721 ttcattgtcg aggctttcat tttcgcgaga caggagattc ccctcgctga ctaatgcatc
     3781 cgatacgcca ttgactggca gggtgcgccg tcggttgagt tcgatttgcc aggcgtgctc
     3841 gtcgcgatgc tgttcgaact cggttacggc attacgcaga gaggtgaaat ccagtggcgc
     3901 cacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt tatactgcaa
     3961 atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctggctgg agatataggc
     4021 atcttccagc accaccactt cacgccaggt gagcaaggtg gaaaaaagca ataccacgat
     4081 aaagcagaga ttaacgacat gaccgggccc caggcggcgt gcgagttttt tcaaccagct
     4141 ctggttttcc atttttgtct cgtgctgcac cctgacgact cccaacctgc ttttttatga
     4201 ttctggcata acccggcgat agcgtaccgc aaaacagcga taattttata atggtggcat
     4261 gataagacgc gactatcatt cattgcaggc attgggacac caggccggat gtgatccggc
     4321 ctggtgttaa tgataccctt tcaaacttct tccgcagaac gactcccctc ccccggaaca
     4381 agctcggttt catgaaaagc ttcccgcttg accccgtacc cgtcatacca acgacactca
     4441 accataccgc tggagtatcc agtgacaatc atccgcgggc cgccctcttt aaccgtaact
     4501 tcctcactaa ccataaagct catactcgcc tccttttttc gagtgaaact tgttcacctt
     4561 agttgaagat ggcgaatttt gcataatagc ctgtgcgagt attaatgtgc ctgaccgcgc
     4621 attttcgcta cggctttcac tcccagcacc acgatgccgc cgatgataaa tccaagaatc
     4681 agatttaaaa cagtcggtaa tatcatcgcc accactgcac tttgctgccc ggcgaaatgt
     4741 tcaatggcgt gatgcaacgg cgcaatacca tgtaccacaa tcccgccgcc gacgaggaac
     4801 atcgccagcg tgccgacaat cgataacgct ttcatcaacc agggcgcaat gatcaataac
     4861 cctttaccta atgcctgcat cagcgcgctg gatttttccg ccagccaata ccccaggtca
     4921 tcaatcttaa cgataacccc taccagaccg tagacgccca cggtcactac cagcgcgatg
     4981 cctgaaagca ccagcacctg attaagcaac ggcgcttctg ccacaatccc cagcgtgatg
     5041 gcgacgattt ccgcagacaa tataaaatcg gtacgaatcg ccccttttat tttgtccttt
     5101 tcaaacttca gcggatcctg cgccgccagc ttctccagac gctgctgact ctgcgccgga
     5161 tcttctttat gtttacgcgc ctccagcata tgcagcactt tctctacccc ttcaaagcag
     5221 agaaacgcgc cgccaatcat caacagtggc gtaatcgccc acgggataaa cgcactgatg
     5281 atcagcgcca gcggcaccag aatcacttta ttgatcagcg aacctttcgc cacgccccag
     5341 accacgggaa gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt gagcgataag
     5401 tcatccccta atacaccggc ggttttcttc gccgccagtt tgcccatcac ggagatatcg
     5461 tccagcagtg tggcgatatc atcgagcaac gtcagtaaac tactcccggc caaaagcatt
     5521 ccctctcatt tttattgttg aagggtgtaa gtatgaagca aaaacggcag ctctgatact
     5581 tgcttcacag gtcaacattt atttaacaat caagtgcaat tatatctctc gccagcagaa
     5641 tgattttcac gtttactatg aaccaccttt ttatttcagc cgtgagggat tatgcgtttc
     5701 cgccagttgt taccgctttt tggcgcgctg tttgcgttgt atatcatttg gggctcaacc
     5761 tattttgtca ttcggattgg cgtggaaagc tggcctccgt taatgatggc gggcgttcga
     5821 ttcctggcag ccggtatttt attgctggca tttttgctac tgcgcggaca caaacttccc
     5881 ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt tattgctggc tgtcggtaat
     5941 ggcatggtga cggttgccga acatcaaaat gttccttccg gcatcgccgc cgtagtggtt
     6001 gcaaccgtgc ccctttttac cctgtgcttc agccgcctgt ttggcattaa aacgcgcaaa
     6061 ctggaatggg tgggtatcgc cattgggctt gccggaatca tcatgctcaa tagcggtgga
     6121 aacttaagcg gcaatccatg gggcgcgatt ctgattttaa tcggctcgat tagctgggcg
     6181 tttggctcgg tttatggctc gcgcattacc ttacctgtag ggatgatggc gggtgcgatt
     6241 gagatgctgg cggctggcgt ggtgttaatg attgcgtcga tgattgcggg tgaaaaactg
     6301 acggcgctcc cttccctttc aggcttcctt gcggtcggct atctggcgct gtttggttcg
     6361 attatcgcca ttaacgctta tatgtattta atccgcaacg tcagtccggc tctcgccacc
     6421 agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg gtacgggact gggtggcgaa
     6481 acgctgtcga agattgaatg gctggcgctc ggcgtaattg tcttcgcggt ggtactggtc
     6541 acgttgggaa aatatctctt cccggccaaa cccgttgtcg cgccagttat tcaggatgca
     6601 tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg ctggcacctt cgccgcagat
     6661 ccactcttcc agacgctcgg taagcgccgc atccgtaagc ttctcgcgcc cacgtaacgc
     6721 acactcccag acaatcagta cgcgccagcc gagttcctgc aagcgactga tatcgcggcg
     6781 atcgcgttca acatttttac ctatcttctc cagccagaac tcggttcgag tcgcaggcac
     6841 tttaaacaga tagcagtgat gatgatgcca gaagcagccg tgggtaaata tcacgcagcg
     6901 atattcatca acgacaaaat ccgggcgtcc gggcagactg gcgtcctgaa cgcgaaacgc
     6961 aaggccctgc ccggttaaca aactggcgag gcgcttctca atcgccgtat cacgcgtggc
     7021 aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga acgtcgacca tgttgtgcct
     7081 cttgctgacg caacgccacc gcctgtttga tttttggctc aagcagtttt gccaccgcgg
     7141 caaagaccgg cacgaccacc gagttaccga actggcgata ggcctgagtg tccgaaaccg
     7201 gaatgcggaa tttcgcttct cccggcgctt caaaacccat taagcgcgcg cattcacgag
     7261 gtgttaaccg acgtggacga tgttgctgat tcagcggatc gtcaaagtct ttctcacccg
     7321 tggccatatc ccagccgcgg tcgattaaaa tttccgcgcc atccttgtaa taacgagcag
     7381 acagcgtacg ggtgacgctt tgcggattgt tcggataaac cattccataa ccgaagccgt
     7441 taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta cttccacagc accggcgtca
     7501 ggatatattt cgcctcaacc atcgggtcca gcagctgcgc cagcgtcact cgctgtgcag
     7561 ggaaacattc gctgatatca cgcagagtaa aatcggcttt cagattcaga tcgcgacgaa
     7621 aacccaccag cacgatgcgt tcacggtgct gcggcaggaa atgtttaccg tcgatgattt
     7681 tcggatcgtc cggcccgtta tctgctgcgt cggcaacgtc atagcccagt tcgtccagcg
     7741 tctgcatgat gatgcggaac gtttttccct tatcgtgact cttcaggttt ttgacatttt
     7801 cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg caccacatca aagaacagcg
     7861 taccctgggt atcgcaggca aaaccgtgcg cccgcccgag cgagttcttt ttcgatacgc
     7921 cagccagcga aaatggctga caagggaaac cggccagtaa aacatcgtgt tcaggaatgt
     7981 gttgacgaat atgttccgcc gccgcctcat cactcacgcc ttcttgatga ctgagggtga
     8041 tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc gcaataatgg ttggctttat
     8101 aagtgcgtac agcatgtttg ttccattcgc tggtaaacac gcactgtccg ccaatcgatt
     8161 caaaaccgcg acggatgccg ccaattccgg caaatagatc gataaagcga aacgcataat
     8221 gcggatgatg ttccggtggt ttgggtaaca acgtttgcag atgggcaaac tctttctcac
     8281 ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt taaaattgcc gcgctccagt
     8341 gattctcacc cacaccatta agctgcgcca ccagcgtttt aacatcataa atttgcagca
     8401 gtttctccag cattgcctgt gcggcattcc cggtgctgta tgaatcggtt actgatatat
     8461 tttcctgcat agatttaacc ggccatctga gatggccgac aggttaacat aattactgcc
     8521 gtttagggcc acgcgctcag ccagcgggtg aaaacgcatc tatcaccttg tgatcgatac
     8581 cttcattctc ccccgccagt tcggcaccga gcttcgccat aaactcgact aaaaattgcg
     8641 cgttgtgctg tgccagctgc ttgcctcttg cggtttgcat ggtttgcggt aatttcagta
     8701 actttgtctg gaaatgatcc agcgcgtagc gtttatcatc aagcgggcga tgctgtgcga
     8761 acggatcttc gccatcaaac agcgccaagc ccaatgcccc tgaaacggca aatacgcgcg
     8821 ccaggccaat cgcccccagg gcttccagcc gatccgcatc ctggacgatt ttagcttccg
     8881 ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc aatggcatga caaacggcct
     8941 caattttctc cgccggaaat tgcgcgaact cttcacggag caggcgacgc gtctcttctg
     9001 ccgccaggat tgaagaacgc tgccgttgcg gatgattttt cgccagggtc acgatatcgt
     9061 gaaaataaca ggcggttaaa atcaccagca tatcgacatc gtcatctgcc gccagtttct
     9121 gtgccgtagc ccagacacgg cgaaaatgac acaagtcatg cgccgcgtcc tgatgttgat
     9181 gatggttttt caaccagtta tcgaactgtg cctgccagtg ttgtaactcc atacgcgctc
     9241 cgttgccaaa gagtcgcaac cttagcagtt tttattcata gcgtctgcaa cgtgcgggac
     9301 gaggtcgggt gtaccatgca atgccgccta acaaggcacc aaccagggcc ataaacagaa
     9361 atccgaccaa tgctcctaac cacttccccg cggtagatcc taaatcactt tcaaatcccg
     9421 acacgggggc atcggggatc cggctcatca cccagatgta tcgcgccatt gcccagacga
     9481 aatagccaca ccatgtatag aaagcccaca aagccaattt gccgccaggg ctgcgagaga
     9541 gtctctcttc catctttgtc gaaccgtatt ccattttgag ataacccgga aaattcagac
     9601 ttagaactat tatgtgataa aagtcacatt tttccacatt ggatgagcca gtcaagactc
     9661 aatcattttt tgaacaaata gataaatttt gcgcaacggc ggtgaataca ggaaaaatcg
     9721 atttgaacga tgttatttat ctttg
//
LOCUS       DACADA010000162         9637 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.164, whole genome shotgun sequence.
ACCESSION   DACADA010000162 DACADA010000000
VERSION     DACADA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.164"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(324..399)
                     /locus_tag="ISB17_003962"
     tRNA            complement(324..399)
                     /locus_tag="ISB17_003962"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Asn,seq:gtt)
     gene            complement(500..2143)
                     /gene="yeeO"
                     /locus_tag="ISB17_003963"
     CDS             complement(500..2143)
                     /gene="yeeO"
                     /locus_tag="ISB17_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAP1981841.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVFSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWAGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2148..2223
                     /locus_tag="ISB17_003964"
     tRNA            2148..2223
                     /locus_tag="ISB17_003964"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2181..2183,aa:Asn,seq:gtt)
     gene            complement(2261..3211)
                     /gene="cbl"
                     /locus_tag="ISB17_003965"
     CDS             complement(2261..3211)
                     /gene="cbl"
                     /locus_tag="ISB17_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAP1981842.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3313..4230)
                     /gene="nac"
                     /locus_tag="ISB17_003966"
     CDS             complement(3313..4230)
                     /gene="nac"
                     /locus_tag="ISB17_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAP1981843.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4557..4632
                     /locus_tag="ISB17_003967"
     tRNA            4557..4632
                     /locus_tag="ISB17_003967"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4590..4592,aa:Asn,seq:gtt)
     gene            complement(4688..5623)
                     /gene="ldtA"
                     /locus_tag="ISB17_003968"
     CDS             complement(4688..5623)
                     /gene="ldtA"
                     /locus_tag="ISB17_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1981844.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5685..6764)
                     /gene="cobT"
                     /locus_tag="ISB17_003969"
     CDS             complement(5685..6764)
                     /gene="cobT"
                     /locus_tag="ISB17_003969"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAP1981845.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6776..7519)
                     /gene="cobS"
                     /locus_tag="ISB17_003970"
     CDS             complement(6776..7519)
                     /gene="cobS"
                     /locus_tag="ISB17_003970"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAP1981846.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(7516..8061)
                     /gene="cobU"
                     /locus_tag="ISB17_003971"
     CDS             complement(7516..8061)
                     /gene="cobU"
                     /locus_tag="ISB17_003971"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAP1981847.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2685 a         2529 c         2038 g         2385 t
ORIGIN      
        1 aatggcaggt cttctttttc tgattaccga agacaaagtg gcaattacgt atagtttccc
       61 gataaagacg cgacagctac atcccgcata aggcattttt ctctttatct ttgtacggta
      121 cttcatggaa cagaattttt gaccttgtga atcgtgatgt ctgttgggga ggggcaattt
      181 gctcactgaa gcgtgagact cgattaagcg cacgaaacac agaaatcaaa aacccggcca
      241 cttttttaca aagtgaccgg gtaaaaataa tttttatttt ttaactgtcc tgagactcat
      301 agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag tgacatacgg
      361 attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac gaggcgaata
      421 ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc gtttgctgaa
      481 ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac ttttgcggct
      541 cggatcgagg gtatttccat agccaacgtc cagtaaccat tcgccagtaa aacagcacgg
      601 cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc cagccaagca
      661 tgattcccag cacataaccg actacaaccc gacaacccca catgctcaac atcgaaaccc
      721 acatggcgta acgggcatca cgagcacctt taaatccagc gggtagcacc catgaggcgg
      781 accaaatagg cataaataaa gcatttagcc aaatcagaat cacaacgacg tgtttaacct
      841 gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt agccaggcga
      901 tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc tctgcttgcg
      961 ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa ccgagcgcac
     1021 ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg gcaataacgc
     1081 tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta aataacactg
     1141 attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta aaattcagcg
     1201 gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc gccagcaccc
     1261 acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca gccccgacaa
     1321 atcccagtcc cgcccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa
     1381 gaatattcag gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg
     1441 ccccgctacc aataagagtg atggcagctg ctggataact gaatacggtc agctccagat
     1501 aagtcaacgc cagtgcttta acgtctgtcg tggcatcacc cgcgacgaaa tcaataattt
     1561 gttcgccaaa atgatgaatt agcgttgcca acagtacggc aaacaacgtc atgatcacca
     1621 atgactgccg cgtcgccacc ctcgctcgtc gtcgatctcg cttaccgaga ctaaatgcta
     1681 caacgacagt agtaccaaga tcgatagctg caaaaaaagc cataatgacc atattgaagc
     1741 tgtccgccaa ccccacgccg gccatcgcat cttttcccag ccagctgacc agaaaagtgc
     1801 tcaggacccc catcaacagg acacaggcgt tctccatgaa gataggaaca gcaagcgggg
     1861 ttatctcgcg ccagaacaac actttgtagc tcttgcgttt agagtgccag cgagtgccgt
     1921 gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa
     1981 accacatttc aaatgatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt
     2041 gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttgggaaat ttaaaaatcg
     2101 ggtttgacaa aatatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc
     2161 agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag
     2221 ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt
     2281 cctcttcact gttttccatc acctggcgct tgatatcctc aactgacagt cctgcattac
     2341 aaagttccag aaagcgccag acatagttac gctgaagttg ccctcgcttc agtcccaacc
     2401 agacagtatt agcatcgaaa agatgccgcg tatccaggcg gattaaattc ttttcctctt
     2461 gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt
     2521 taatgacatc agaatcctgt gcacttaata caatatctgc cggcaaacct ttgcgggcaa
     2581 atgcgtcatc aatgcgtgag cgccccgtaa tcccctgtcg gtaagtaatt aacggccact
     2641 tcgctattga ttccagcgtc aacggtgtaa tttgcgtcaa gggatgatcg agtggaacaa
     2701 gcaaactatg gtgccaacga aaccacggga aggcgacgag ctgcgggtca ttactcaaac
     2761 gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg
     2821 gtgtaccctg gattagctcc agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga
     2881 cctcaggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acgccagacg
     2941 tatcgttggt aaacaggtct gcgagccgac gaacattact ggcttcattc agaatgcgtt
     3001 ctgcaatgac cagtaatgct ttgcccggtt cagtcatgcc cagcagtcgc ttacctcgtc
     3061 gaacaaatat ttcgatgcca agttcatcct ccagttcccg aatatgacgg ctaacgcctg
     3121 actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct
     3181 cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc agatatcccg
     3241 ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc
     3301 ccgatataac gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat
     3361 cactgacatt aacaactctt tcactgcctg tgcctgtggt gacaagtttg ctctggcggg
     3421 taaatttaag gacaaagaga gactcattga aggagtggta atgcgtgaca tccacccatt
     3481 tactgcgcca cataacgaac gcgcagccga ttcaggtaat actgcaacgc ccatgccgct
     3541 ggcaatcgct gcggtaagcg tggcaataga ctcaatttca ccaatgactt ttgccgtgag
     3601 ccgccgtagg gaaaaagcct catcaacacg aagtctaaca gcgctgtaat cactggggag
     3661 aaaaaggttc atttgcgcaa tagcattaac atcaacgctt tgccccgggc aatcttgagt
     3721 tcctaccagg aaaagatctt ctttcagcaa agcctgactg gataccccag ccacagggga
     3781 atgctcataa atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc
     3841 accactattt tcatgaagat agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa
     3901 taagggcatg gtgatggatg acgcagcggt tcctggcgca aagccaatcg agacttgccc
     3961 cgataatgac tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat
     4021 ggcccgtgca tgggtataga gaatttttcc ggcgcctgtt ggcgtaacgc cccgttttgt
     4081 gcgaatcaaa agctgttgat ttaactcacc ttccagtgtg gcaacctgct ggctgagcgc
     4141 tggttgtgcg atatgcaata cttcagcagc ctgggtcagg ctaccaatat ctacaatttt
     4201 tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga atcggcccaa
     4261 gtgccgccat tacttacaac cagaatgcaa gatgcttgcc agttctattt tagcgttgat
     4321 gcgcaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg gcccttttta
     4381 tcagcagtcg cattcacctg ccctataaaa ggggggtatg caccacgatg gttcattacc
     4441 caataagatt gaaagctcac cactttgttg aaattgtcag caaacaaaca aaaaaatgca
     4501 tttcaccctt tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct
     4561 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     4621 tcagaggagc caaattcaaa aaagcctgct ttcgagcagg ctttttactt tctaattacc
     4681 aacgctctta aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgat
     4741 gttgcaacgc agcatttgcg cgatttacat caacttcttg cccgttgata aacgcccgca
     4801 aagatggggt taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgcg
     4861 acagcggctc atgaacttcc agccactttg aaccatccgg ttccgttgtg tatttcactg
     4921 gctgatcaat aatttgtaca cgagtgccta caggaacatt atcaaacaga tatttgatat
     4981 cgtcattacg cagacgaata cagccctgac ttacgcggag cccaatacca aaattggcat
     5041 tggtaccgtg gatggcatac aacttgccaa tataaatcgc gtacagcccc atggggttat
     5101 cgggccccgc aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccggg
     5161 tgttcggcgt tggcgtccag gttggtgctt cttgtttacg ttcaacggta gtcacccagt
     5221 tacgcggggt ttctcgccca gcctggccga taccaatagg aaagacttcc actgtattac
     5281 tgtctggcgg atagtaataa agacgcattt cagcgacgtt aaccacaatc cctttacgaa
     5341 cagtggcggg caaaatcagt tgctgcggaa tggtgagttg tgagccaggc ttcggcaaaa
     5401 aaacatcagc gcccggattc gcttccagca tattacttaa cccttgcccg tattgtgcgg
     5461 caaaagtctc cagcggctga gtattgtgat caggaacagt tacagtaagc gactgcccca
     5521 ctaaacggct accctctgga ggtaatggat aagttaccgc caggctattt tggctggcaa
     5581 aaagcagagc aaatgagcaa agaatattta cacgacgcat catgtccctt tcctaagtcg
     5641 cgaatactat ccgataagta tagcttttat cagacttttc gtttttagct gttcaaatca
     5701 gaagtcgtat tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttgtat
     5761 atcgcacaag cagcttcgat gatggacatc gccagagctg caccactccc ctcacctaaa
     5821 cgcatatcca tattgagata aggctccagc cccaaatgcg agagcgctat acgcgcgcct
     5881 ttttctgccg acaagtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag
     5941 gctgcgagcg cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa
     6001 gcagcaccta acatcactcc agctattccg accaaatcaa atccacccac ttttgccagg
     6061 acatcaacac catcctgagg atttggttga ttcaacgtaa tcgcccgacg cacaacatca
     6121 attttattag ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga
     6181 tcccggccag tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt
     6241 tcacctacac caaacagcgt gacaccgttt tttgccagct cccgcgtata acatatgacg
     6301 tccaaaagca acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg
     6361 ctacctcgtg cgacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca
     6421 acatcaatta cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt
     6481 ccacgggtca tattttcagc ctgtatggct gtcacttctt ttggagaaat agcgacccct
     6541 tcctcccaga cgccgtgatc ggcacacata accagtactg cttttttgcc cacatgcggt
     6601 atgccattca accccggcat tcctgccagt tgtatggcaa gcgcctccag ctttcccagg
     6661 ctaccaacag gtttgagtaa cccgtcaaca tgccgttgtg cacgcgacat agcggcagaa
     6721 ttgatggctg ggatcgtatt cagtaaatcg gcaagtattt gcatgtcacg tctctttata
     6781 gcagtgccag caggaaaacc agttcaccaa actctatggc tgctcccagc gtatcgcccg
     6841 tttgcccgcc cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca
     6901 ccaccatagc agccacacca tgcatacccg gcaataatac ggcagcaaaa atagcggcta
     6961 agccgagtgt gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc
     7021 cttcttcccg cgcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg
     7081 cacatgctgc cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaaca
     7141 ccagaatctt tgccagtacc acaaaaatca atgccagacc gccgtgggtg cctaaacggc
     7201 tatcacgcat gatttccaac atgcgatcgc ggctacgtgc agaaaatacg ccatcacagg
     7261 tatcagcgag gccgtccaga tggaatcccc cggtcatcag cgccagcaca agtacgctaa
     7321 acagtgccgc cagtggtacg ccacaccatg cctgcagcac catgaagacc agcccgctaa
     7381 tcgcgccaag caataatcca atcaaaggaa aagtaataat accgcgagaa taatgctcga
     7441 aatccagtcc ctgggaccag cgacgcggta caggcagacg cgtaataaat gagagcatcg
     7501 cccaaaataa tttactcatt tgatttttac tccaataccc gaaaccacca gccatacttc
     7561 atttgccgca gcggccaact gctgatttac ccgcccggca atatcacgaa aatgtcgtgc
     7621 cagacgactc tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc
     7681 ggggcaacgt tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat
     7741 cgcctgataa tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt
     7801 caccattgtg gtaacgcatt ccagcaacac agcctcattc gggttaatgt ctgcatgaat
     7861 taattcatca agatgttgcc agcgctccac tgtgcgccag tgctccgggc ggctttgccg
     7921 atgatgttct atccgtgcag ccatctcatc atcaaggatt tgcgaggtag cgatatacag
     7981 aacctgtgaa gagtccccaa taagcacctc tgcgtggcga ctcttcccgc tccgtgcgcc
     8041 tcccgtcacc aaaatcatca taccgactcc tgattcaaat gtatgatggt attcattgaa
     8101 cagagcaatg gatcccccac cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc
     8161 gtaatataat taaaaatcca gcattctcaa tccattacga aataagatgg cattacggaa
     8221 tagccagact ttctcgtgtt aacgtccgac aaaatgcgtc attgacagcg gtttctgtgg
     8281 attgacacat ttcattcaga taatgaatta atgctactgc cggaccaacc agcaaacggg
     8341 cattgtgttc tgaaatccag gctattgatt caacgtcagc gacagatctg cgctgtaaaa
     8401 gaagttgtaa aacaacgcaa taaaattatc cggaatggtg gcgactatgc actagaacac
     8461 cacaatttcg ctctctcggt aaggcactgt cacagcattc aacagaatgt gacttgccag
     8521 atttattagc gccaccagat gtttaaccgg gtaaccacga cccagtcggg gatatattcc
     8581 atcttctcgc tgacatcatc aagaataaaa aggtttatca cactcagagc aacgttcttc
     8641 agcaggcggg ataattcttc aacatttaaa agatgtgtta ttaaaggcat acaaactgga
     8701 cgtattgtat tttcttttgg tggttgacct aaggtagcag tttatcctga tgcgctgaga
     8761 tttctgtaat atctacgtca acattctcct cgaatagaaa atgcagcctc ggcaagtgat
     8821 tcacattttt taacaaagcg ttgttagcgt gccattttca gaagctctcc aagaaagcga
     8881 attcttttct caaattctgc attctcagac acagctttct actccatggc aatggcccaa
     8941 atacgaagtt gctcaggatc attagtggtg ctgagaagtt aaataaccat gcggagtaca
     9001 actcgtcatc acgtatttat atatcatgaa gctattgttg tgttttgtac atcagtagat
     9061 gtattctgat atactccttt ttctagacat aacctttcac ctgcttgcaa agcttctgtg
     9121 ttctgacatt gccaaattgt tgcaattctg tatccagcct tctttcagtc atagcttcgg
     9181 gccgcgataa gactcactga tctgaccctg attcctcttg cagactttat agaccaatta
     9241 aaatgcagtt tctgcaggtc aacgtctgac catcattgtc atcactctgg ccattagagt
     9301 aaccttctgc attcatcctt ttgtaaaaag tttatgtcag tatcagcaat taaccggacc
     9361 tgatactgat atgagtctta ccgcatatac ggtcaatttc agcaattaat tacattatcc
     9421 acgccaaagt gtttgtcatc gcaatgatgg taccttcttt cagacaccat tttttcaact
     9481 ccgttttcca cggaccgcac tcttatgtca agagtacggt ccgtggatac aaccagagac
     9541 cgactgacac gagtcagagg aaacgacgga tatgttcagt cgtaaaatat ctatcaaaaa
     9601 acatgattaa ggtcaaaaat gtttgatatt tacaatt
//
LOCUS       DACADA010000163         9630 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.165, whole genome shotgun sequence.
ACCESSION   DACADA010000163 DACADA010000000
VERSION     DACADA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.165"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            13..513
                     /gene="tssB"
                     /locus_tag="ISB17_003972"
     CDS             13..513
                     /gene="tssB"
                     /locus_tag="ISB17_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAP1981848.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            572..2110
                     /gene="tssC"
                     /locus_tag="ISB17_003973"
     CDS             572..2110
                     /gene="tssC"
                     /locus_tag="ISB17_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAP1981849.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2128..3465
                     /gene="tssK"
                     /locus_tag="ISB17_003974"
     CDS             2128..3465
                     /gene="tssK"
                     /locus_tag="ISB17_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAP1981850.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3462..4127
                     /locus_tag="ISB17_003975"
     CDS             3462..4127
                     /locus_tag="ISB17_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HAP1981851.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            4140..5792
                     /locus_tag="ISB17_003976"
     CDS             4140..5792
                     /locus_tag="ISB17_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP1981852.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5850..6341
                     /locus_tag="ISB17_003977"
     CDS             5850..6341
                     /locus_tag="ISB17_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HAP1981853.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6532..9168
                     /gene="tssH"
                     /locus_tag="ISB17_003978"
     CDS             6532..9168
                     /gene="tssH"
                     /locus_tag="ISB17_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HAP1981854.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLGWDEEDGIVLEFDEK"
     gene            9180..>9630
                     /locus_tag="ISB17_003979"
     CDS             9180..>9630
                     /locus_tag="ISB17_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1981855.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT         2149 a         2712 c         2788 g         1981 t
ORIGIN      
        1 gaggaaaagg atatggccga cagtttccag aatgaagttc ccgccgctcg tgtaaatatc
       61 aagcttgacc tgcatacagg caatgctaaa aagaaagttg aactccccct caagcttctt
      121 gccgtaggcg attacagtaa cggaaaagag cagcgtccgc tgtccgaacg ggacaagatt
      181 gatatcaata aaaacaactt caacagcgtc atggctgagt tttcgcctgc ggttaattta
      241 acagtagaag atacgctaaa cggaaacggt aatgaacaaa atatcgcgct tgaatttaaa
      301 agcctgaagg attttgagcc cgaacaggta gcaaaaaata ttccacaact tcgtgtatta
      361 ctcgctatgc gcaatttact acgtgacctg aaatccaatc tgctggataa cgcaacgttt
      421 cgccgtgaac tggaaaatat cctgaaagac ccgacgctca gttcagaact gcgtgatgaa
      481 ctggcgaaaa ttgcgccgca ggagaacgtt taacccgaac gcgtattcag gttaaaccgg
      541 agaagaaagg atcactgaaa aggaaatgct gatgtctgta caacaagaac atgccacctc
      601 tgaaactgca acactcacca ccactgagtc cggcggcgtt tatcagtccc tgtttgataa
      661 aattaattta accccggtct cttccattca ggaaatcgat tcatggcaaa acagcgaaac
      721 gctggccgat gcctcacccg atgagcgcgt gacggcggct atccacgttc tgctttcctg
      781 tctggcgaag tctggcgagg acgtggttaa actcgacaaa agcctgctgg attttcatat
      841 cgacgatctg gatcagaaaa tcagtaaaca gcttgatgca gtcatgcacc atccggaatt
      901 ccagaaagtc gagtcgctct ggcgcggcac atggttcgtc gtacagcgca ctgattttcg
      961 caaaaatgtc agaattgaac tgctggatgt cagtaaagaa catctgcggc aggactttga
     1021 tgattctccg gaaatcattc aaagtggttt atatcgccat acatacattc aggagtacga
     1081 tacgccgggt ggcgaacctg tagcctcatt aatatccagc tatgaattcg ataacagccc
     1141 gcaggatatt gccctgctgc gtaatatttc cagagtctct gctgcatcac atatgccctt
     1201 tatcggttcc gtcggaccga agttcttcct taaaaactcg atggaagaag tcgccgcaat
     1261 taaagatatc ggcaactatt ttgaccgcgc ggaatatatc aaatggaagt cgttccgcga
     1321 taccgacgac agtcgctacg tgggattagt gatgccgcgc gtgctgggcc gtctgcccta
     1381 tgggccggac acagtgccgg tacgcagctt taactatgtg gaagaagtca aaggcccgga
     1441 tcacgaaaaa tacctctgga caaacgcctc gttcgccttt gccgccaata tggtgaagag
     1501 ctttgtgaat aatggctggt gcgtgcagat ccgtggtccg caggcgggtg gcgcagtggc
     1561 cgatctgccg atccatcttt acgatctcgg caccggcaat caggtcaaaa ttccgtccga
     1621 agtgatgatc ccggaaaccc gcgaatttga atttgccaac cttggcttta ttccgctctc
     1681 ttattataag aatcgcgatt acgcctgctt cttctcagcg aactctgccc agaaaccggc
     1741 gttgtatgat accgctgacg ccaccgccaa cagtcgtatt aacgcccgtt tgccctatat
     1801 tttcctgctg tcccgcattg cgcattacct gaaaatcatc cagcgcgaga atatcggtac
     1861 caccaaagac cgccgcgtac tggaactgga gcttaacacc tggatccgca cactggtgac
     1921 ggagatgacc gatccgggtg atgaactgca ggcgtctcac ccgctgcgcg acggcaaggt
     1981 tatcgtggaa gatattgagg acaatccggg cttcttccgc gtcagactct ttgccgtgcc
     2041 gcatttccag attgaaggga tggatatcaa cctgtcactg gtttcccaga tgccgaaagc
     2101 gaaagcctga ccgggccgtg gtgtgatatg aaaattcatc gaccactgtg ggctgagggg
     2161 acattcctgt cctctcagca gtttcagcag caggcccgct gggaagcgtt cagcaatgac
     2221 agcattgcgc aactttgtat tcgtcatccg tgggggatcg cgaatgtgct ctttgaccgc
     2281 gatgccctga cgtccggaaa gctgaaaacg caggctgtac gtctgcgttt tgctgatggc
     2341 acgctgatcg attcagacgt gagtgacgtt ctgccgctcg cctgcgatct gcgcgcgctg
     2401 aagaacgaca gcgcgattgt cctgctggcg ctgccgcttg cgcatggtaa cggcggtaat
     2461 ctcggtcagg gtgagcagac tgaaagaccg ctgcgctatc gtcaggaatg gcaaaaggtg
     2521 caggatatct acggcagtga cagtgaagat atggccgttg agcgtcatgc gctgtccctg
     2581 cgctttgctc atgataacaa tcaggattac atcacctgcc cactggcgcg tctggtgcgg
     2641 gatgttcagg gcaactggac acaggatgag agctatattc cgccgttact ggcgtttaac
     2701 gcccatgacg ggctggtaca gcgtctggac acgctgctgt tgcagctacg ggcgaagtgc
     2761 cagcgtctga tggcgatgcg ccgggaaagc aaccagcgta tggcggactt cgctgtcgcc
     2821 gatgtctccc tgttctggct gcttaacgcg ctgaactccg ccgagccggt actgagtgat
     2881 ttcctgcgct acccggcggt tcatcccgaa ctggtctggc gggagctggc gaggcttgcc
     2941 ggtgcgctgc tgacgttttc ccttgaacat aacgtcagcg ccgttccgcc ctatgtccac
     3001 gaatcgccgt ccatcgtctt tccgccactt ttctccctgc tcagcgaact gctggaagcc
     3061 agcctgccgt cacgggtgat cgcgctggat ctggcgtccc tgccgggcaa ccgctggaaa
     3121 gcagacctgc acgatccgcg cctgcgggaa gaggccgact tttacctgtc cgttcgttcc
     3181 tctctgcctg cgcatcaggt actgcaccag ctaccgctgg tctgcaaaat cggtgcgcct
     3241 gatgacgtca cgctgcttat caatgtggcg ctgaatggtg ttccactggt gccgcttacc
     3301 tcagttccgg cagcgctgcc actgcggctg gagaaccagt attttgcgct ggatatgcac
     3361 agtgacgcgg ctaaatcgat gctggagtcc gggtgctgca tgatttatgc gccgggaaca
     3421 atgggcgacc tgaaacctga actgtttgcg gtgctgcgca catgaaaaaa gagacggata
     3481 tcaatatcga cgcgctgctg cgcgacacgt ttctgacggt ggtggaactg cgtcagggaa
     3541 ccaccgtgcg tcacggtatg gagctgtatc gccactgcca gcggcaggtg gaactggtcc
     3601 gcgaacgcct gaaagatgcc gggttcagcc gggagagcgt ggagcacatt acttacgcgc
     3661 agtgcgccct gctggacgaa acggtcctga gccgtggcgg catggacgac ggtcaggcca
     3721 tctggatgaa ggatccactc cagtcgcatt tcttcaacac tctgcaggcc ggtgagctgc
     3781 tgtacgagcg gatgaagcag gtgctacagg agcccgcccc cgcgcaggcc gtgctgacct
     3841 gctttcaccg tgtcctgctg ctgggatttc gcgggcgtta tcaggatccg gcagccccgg
     3901 agcgcgacca gcttatttcg acgctcaacg ggcaggtcgc gccatttggt gtgctgccgg
     3961 aaacggcggt actgaacgtg ccgttatccc cccgtcagca ccctttgctg cattcgccgt
     4021 ttttctggct ggtgacgctc acactggtac tggccggtgt ctggtggggg ctacattact
     4081 ggctgaacgt gctggtggat gaactgctgc cgcaaagtct gcgttaaagg gagtcgtgag
     4141 tgaggaatgc gctgaaacag acggttttgt tatggactct gttgttgctg ctgactacct
     4201 ggctgggttt ctttgctgta tctgcggtcg tgaagtatgc cgtggtagtg attcttgtcg
     4261 ccgtcacagc ggcggtgatg gcgtactggc ataagaaaaa gggacaggtc gtagcggacg
     4321 acgtatggcc gtcatcgcta ccgccggaaa cctaccgaca gccggtagtg ctggtctgtg
     4381 gcgatatgtc ggcgcacctg ttcactgaca gcccggtccg gcaggtttca gaagggctgt
     4441 atctgcctgt ttctgatgaa gaacagcttg tggcgcaggt ggagcgatta ctgacccttc
     4501 gcccggcgtg ggcatcgcag tttgccgtgg cgtataccgt catgcccggc atgtaccggg
     4561 atgcggcggt tctgaccgga caactgcgcc ggttcgctca cagtatggcg acggtgcgtc
     4621 gccgggcggg cgtaaacgta ccctggcttc tctggagcgg gctgtccggc tcgccgttgc
     4681 cggagagagc gaattcaccg tggtttatct gtaccggcgg cgaagttcag gtagcaacat
     4741 ccgcagagac caccatgccc gcgcagtgga ttgcacaatc cggcgcacag gaacgcagtc
     4801 agcgactctg ttacctgctg aaagccgaaa gcctgatgca gtggctggat ctgaatgtgc
     4861 tggcggaact gaacggcccg gaggcgaaat gtccaccact ggcgatgacc gttgggctgg
     4921 tcccctcgtt gcctgcggtg gataacaacc tgtggcagtt gtggatcacc gccagaaccg
     4981 gcctgacgcc ggatatcgcg gacaccggca cagacgatgc gctgccattc ccggatgccc
     5041 tgttacggcg gttgccgcgt cagtcgggct ttaccccgct gcgacgggcc tgcgtgacca
     5101 tgctgggcgt caccaccgtg gcgggtatcg ccgcgctgtg cctgtcagcc acggcaaatc
     5161 gccagttatt acggcaggtc ggtgacgatc tgcaccggtt ttatgccgtc ccggcggaag
     5221 aatttatcac caaagcccgt cacctgtcgg tgctgaaaga cgatgcggtc atgctcgatg
     5281 ggtattaccg ggaaggagaa ccactacgtc tcggtctggg gttatacccc ggtgaacgca
     5341 tccgccagcc ggtattacgc gccattcgcg actggcgtcc gcctgagcaa aaaatggacg
     5401 tgacggcttc gcttccggtt cagaccgtgc gtcttgacag tatgtcgctg tttgacgtcg
     5461 gacaggcccg cctgaaagac ggctcgacaa aagtgctggt ggatgcgctg gtgaacatcc
     5521 gggcaaaacc gggctggctg atcctcgtgg ccggatatac cgatgccacc ggcgatgaaa
     5581 aaagcaatca gcagttatcg ctgcggcgtg ccgaagcggt gcgcaactgg atgctgcaga
     5641 ccagcgacat cccggccacc tgttttgccg tacagggact gggcgagagc cagcctgcgg
     5701 cgaccaacga cacgccacag ggccgggcag tcaaccggcg tgtcgaaatc agtcttgttc
     5761 cgcgttctga cgcctgtcag gacgtgaaat aaaacatacc gccggaagaa ggcggtgctt
     5821 caatcacact aacaaggaga gtaattctca tggctattcc tgcttatctc tggctgaaag
     5881 atgacggcgg cgcggatatc aaaggttccg tggacgttca ggggcgcgaa ggtagcatcg
     5941 aagtggtggc gctggatcac gatgtgtaca tcccgaccga caacaacacc ggcaaactga
     6001 ccggtacccg tactcacaag ccctttacgt ttaccaaaga aatcgatgcc tccagcccgt
     6061 atctctacaa agctgtgacc accggccaga ctctgaaaac ggcagaattt aagttttacc
     6121 gcatcaacga tgccggtcag gaagtggagt acttcaacat cacgcttgat aacgtcaagc
     6181 tggtcagagt cgctccgctt atgcacgaca tcaaggatcc ttccagagag aagcataacc
     6241 acctggaacg tattgagttc cgctacgaga aaatcacctg gacttacaaa gacggcaaca
     6301 tcattcattc cgactcgtgg aatgagcgtc cttccgcctg ataactgcgg cagacaggaa
     6361 actgtctgcc tttttgttgt gtgctttgtc cagtgccgca ggcaccggta ctgagcaaag
     6421 catacccaca atctggtgaa acctgatggg cctgagtgag gtaacggcta tgggcggtag
     6481 cgtgcctgaa cgcggatcgc aggaatacag aacacgataa ggaaaccttc tatgaccgga
     6541 aatcaccccg ccgcgctgct gcgtcgcctt aacccatact gtgcacgggc gctggacgct
     6601 gccgcctcac tgtgtcagac ccgcgcccat gcggaaataa ccattgaaca ctggctgctg
     6661 aaactgctgg agcagggaga aggcgatatc acggtgattg cccgccgcta tgaatgggat
     6721 atcgacacgc tctggcagtc tctgctggca catctggaca ccttaccccg cccggtccgc
     6781 gaacgtcctc aactttctga accgctggca gcgcttatcc gacaggcgtg gctgatagcg
     6841 tcactggaag gcgacgatcc acaaatccgc agccagcatc tgctgatggc gctgacagaa
     6901 aaaccgatgc tgcccgcctg taatgacctg tgggtattgc tgagcctgag ccgtgtgcag
     6961 cttgagcggc tgcgtcccct gctggatgcg cagtcggatg aatgtccggc acgtcagcca
     7021 caggtcaccg aaccgctgac atccgcgcaa cccggggcgg caacgacgga cgcaccggca
     7081 aaaacgctga cggggaaaca ggatgacgcc ctgctggcgg tgcttaaccg ctttaccgaa
     7141 gacgtgacgg aaaaagcccg cagcgggcga atcgacccgg tattcgggcg cgacacggaa
     7201 attcgccaga tggtcgatat cctctcccgt cgccgcaaaa acaacccgat tctggtggga
     7261 gaaccggggg tgggcaaaac cgcgctggtg gaagggctgg cgctgcgtat caccgaaggc
     7321 aacgtgccgg acagcctgaa aacggtgcat atccgcacac tggacctcgg tctgttacag
     7381 gctggcgcgg gcgttaaagg tgaatttgaa cagcggctga aaaatgtcat cgatgcagtg
     7441 cagaaatcac cggagccggt actgctgttt attgatgaag cccataccat tatcggtgcg
     7501 ggtaatcagg caggcggcgc ggatgcggcg aacctgctga aaccggcgct ggcaaggggc
     7561 gaactgcgca ccatcgcggc gaccacgtgg agcgaataca aacagtattt tgagcgcgac
     7621 gccgcgctgg aacgccgctt ccagatggtg aaagtcgacg agccggatga tgacaccgcc
     7681 tgcctgatgc tgcggggact caaggcccgc tatgcacagc accacggcgt acatatgctg
     7741 gacagcgcca ttcagaccgc cgtgcgcctg tcgcgccgct atctgaccgg acgccagttg
     7801 ccggacaaag cggttgattt gctggatacc gccggggcaa gagtccgcat gagccttgac
     7861 actctgccgg agccgttgac gcagcttcat gcgcgactgg cggcgctgga tatcgagcgg
     7921 gaggcgattg agcaggacag cgtgttttat cccgaaacca gcccggagcg gctggcggaa
     7981 ctgaccgatt tgcgtgatga gctacaggca gaagccgggc atctggaagc gcagtatcag
     8041 caggaaaagg cactggcgca gcagattatg acgttgcgtc aggaaggaac agacagctct
     8101 gaactgcaac agcaactgcg gacgcatcag ggctttgcac cgctgctggc gctggatgtg
     8161 gacgcccgcg ccgtcgccac ggtggtggcg gactggaccg gcatcccgct ctcttccctg
     8221 ctcagggacg agcagagcga cctgctcagt atggaacaga gtcttgaaaa ccgcgttgtc
     8281 gggcaaagcc cggcgctctg cgccatcgca cagcggctgc gggcggctaa aaccggcctc
     8341 acgccggaga acggcccaca gggggtattc ctgctgaccg gccccagcgg caccggcaaa
     8401 accgaaactg cgctcacact ggccgacacc ctgtttggtg gtgaaaaatc ccttatcacc
     8461 attaatcttt cggaatacca ggaaccgcat accgtttccc agttgaaagg ctcgcctcca
     8521 ggctacgttg gttacggtca gggtggcgta cttaccgaag ccgttcgcaa acgcccttac
     8581 agcgtggtgc tgctcgacga agtggaaaag gcgcatcgcg acgtgatgaa cctgttctat
     8641 caggtgttcg accggggctt tatgcgcgac ggcgaagggc gggtaatcga cttccgcaac
     8701 accgtcattc tgatgaccgc caatctgggc agcgaccaca tcatgcaact gctggaggaa
     8761 aaaccggacg ccacggacgc agacctgcat gaactgctgt atccccttct gcgagaccat
     8821 tttcagcctg cactgatggc gcgttttcag acggtgattt accgcccgct gggacaggag
     8881 gcgatgcgca ccattgtgga aatgaaactg gcgcaggtgg tccgccgtct tcaccagcac
     8941 tacggactgg aaacggaaat caacgacagc ctgtatgacc agcttaccgc cgcctgcctg
     9001 ctgccggata ccggggcgcg caatatcgac agcctcctga accagcaaat cctgccggtc
     9061 ttaagccagc agttgctggc acagcaggcc gtgcatcata agcctgcccg actgcggctt
     9121 ggctgggatg aggaggacgg gattgtactg gaatttgatg agaaataatg gaggctctta
     9181 tgaacgtaca gttcttcgac cacgcacacc acaagctcaa aattcgcggc ctgaaaagcc
     9241 cggtggacgt gctgactttt acaggccacg aacagctcag ctcacccttc cgctacgaca
     9301 ttgagtttac cagtaccgat aaagccatcg agccggagtc ggtactgatg caggacggcg
     9361 cattcagcct gagcgccccg ccggtacagg ggatgccggt gcaggtcccc ctgcgcaccc
     9421 tgcacggcgt cattaccggc tttaaacacc tctcctcgtc acaggacgaa gcccgttacg
     9481 aagtgcgcct tgaaccccgt atggcgctgc ttacccgcag ccgccagaac gccatctacc
     9541 agaaccagac ggtgccgcaa atcgtcgaaa aaatcctgcg cgagcggcac cagatgcgcg
     9601 gtcaggactt cgtgtttaac ctcaaaagcg
//
LOCUS       DACADA010000164         9623 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.166, whole genome shotgun sequence.
ACCESSION   DACADA010000164 DACADA010000000
VERSION     DACADA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.166"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(35..613)
                     /gene="mocA"
                     /locus_tag="ISB17_003980"
     CDS             complement(35..613)
                     /gene="mocA"
                     /locus_tag="ISB17_003980"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAP1981856.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            717..1487
                     /gene="yqeC"
                     /locus_tag="ISB17_003981"
     CDS             717..1487
                     /gene="yqeC"
                     /locus_tag="ISB17_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAP1981857.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            1535..3160
                     /locus_tag="ISB17_003982"
     CDS             1535..3160
                     /locus_tag="ISB17_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAP1981858.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLHQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3201..4133)
                     /gene="arcC"
                     /locus_tag="ISB17_003983"
     CDS             complement(3201..4133)
                     /gene="arcC"
                     /locus_tag="ISB17_003983"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1981859.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4181..5566)
                     /locus_tag="ISB17_003984"
     CDS             complement(4181..5566)
                     /locus_tag="ISB17_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HAP1981860.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5619..6830)
                     /locus_tag="ISB17_003985"
     CDS             complement(5619..6830)
                     /locus_tag="ISB17_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAP1981861.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6888..8084)
                     /gene="dpaL"
                     /locus_tag="ISB17_003986"
     CDS             complement(6888..8084)
                     /gene="dpaL"
                     /locus_tag="ISB17_003986"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAP1981862.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8142..9329)
                     /gene="ygeW"
                     /locus_tag="ISB17_003987"
     CDS             complement(8142..9329)
                     /gene="ygeW"
                     /locus_tag="ISB17_003987"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAP1981863.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2258 a         2341 c         2502 g         2522 t
ORIGIN      
        1 taaatatttg aacgcaatcg tttaactacg ttttctaaat ttcagtatac cttttcttcg
       61 ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatttcga
      121 cggaataatg ttctcccata aggagtgcct gacgcatatt ggtgacattg gggcgctgga
      181 ttgcctgcat caggcatggt tttgacacta aaatcggatg gccggggatg ccattatgga
      241 gtggcagtat tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga
      301 gggttggcat atctccgtgg gtgagaaaac aatgttctgt ttgcaccgcg ggtactgcgg
      361 cctttactga tgtcagtaaa ccctgcgcat aatctgggtt gtggataata gtgatattgc
      421 tctggttcgc ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc
      481 ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag aattgttccc tgttgccagg
      541 gtaacatcat tttccattgc cccattcttg atgataatcc tgcggcggta attatacagt
      601 cgatggctga catagtgatt ccattattta caggagctaa taacgcctga taattgcgtt
      661 acttcctgat ggattacgtt gagggtaatt tatcccagag aggtccataa agacgcgtga
      721 aaagtataat tgacccatcg gcattattca ttgatttagg cgcgcagaaa cgtcccactg
      781 tgatttctat tgttggtgct ggggggaaaa ccagcttgct tttttggctg gcagaactgt
      841 tccaggcgag tggcaggcgt gtattaatca ctacgacgac acatatgttt atgccaacat
      901 ctcactggcc cgtggttttc tgtcgtgatc ccgccatgct tcctcatgcg tcttttatat
      961 cccccatttc attttgtttt cactgctgga aagcgaacca gggaaaagtg cagggattta
     1021 cgccagaagc gattgatgca ctggtacaac gaccagagtg tgacgtaatt ctcattgagg
     1081 cagatggctc gcgtggaatg ccgttaaaag cgcctgatga gcacgaacct tgcataccta
     1141 aaagcagttg ctgcgtgatt gctgtgatgg gaggacatat tttgggtgcg aaagtgagca
     1201 cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac tgggttaaca cctgatgcac
     1261 ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc tcagggcgcg tttaaaaacg
     1321 taccgcaagg ttgtcggcga gtctggttca ttaaccgttt ttctcaatgt gagaatgcga
     1381 ttgcgcaaag cgagctcctt caaccgctgc aacaacacaa cgtagaggca atctggctgg
     1441 gcgatataca agaacatcct gcaatcgcgc gcagatttgt gaattagcgg cccgggtatt
     1501 ccgggttact gaaaggatat ccattgaggt tggtatgaat attttcacag aggctgcaaa
     1561 actcgaagag caaaattgtc cgtttgcgat ggcgcaaatt gttgatagcc gaggctcaac
     1621 cccccgccat tctgcacaaa tgttagtgcg cgccgatggt tctatcgtcg gtacaattgg
     1681 tggcggaatg gttgagcgga aggtgattga agagtcgctt caggcattgc aggaacgtaa
     1741 gccgcgatta ttccatggac gtatggctcg taacggtgcg gatgctgtcg ggtcagattg
     1801 tggaggtgca atgtcagtat ttatcagcgt ccatggtatg cgtccacgtc tggtgttgat
     1861 tggtgcgggg catgtcaacc gggcgatagc ccagagtgcg gcgctattag gatttgatat
     1921 cgccgttgcc gatatttatc gcgaaagcct caatcctgaa ctattcccac catcaaccac
     1981 gcttctccat gctgagtcgt ttggtgcggc agtggaagca ctggatattc gccctgataa
     2041 ttttgtcctg attgccacga ataatcagga tcgtgaagcc ctcgacaaac tcattgaaca
     2101 acccattgca tggctggggt tgctggcaag tcgccgcaag gttcagcttt tcctacacca
     2161 attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt ttacatgcgc ccgttggtta
     2221 caacataggt gcggaaacgc cgcaggagat cgccatcagc gtgctggcag aaatattaca
     2281 ggtgaaaaat aacgcgccgg gtgggctgat gatgaagcct tctcatcctt ccggacacca
     2341 gctggtggtg attcgcggtg cgggggatat cgccagtggt gtagcgctac gtctgtatca
     2401 tgcgggtttt aaagtgatca tgttggaagt ggaaaaaccg acagtgattc gttgtaccgt
     2461 ggcgtttgcc caggccgtgt tcgatggcga aatgacggtc gaaggcgtca ccgcccgcct
     2521 ggcaaccagc tctgcggaag cgatgaaact taccgaacgc ggatttatcc ctgtgatggt
     2581 agatcccacc tgttcattgc ttgatgaact gaaaccgctt tgcgtggtgg acgctattct
     2641 ggcgaaacag aatttgggaa cgcgggcaga tatggcacca gtaacaatcg cgcttgggcc
     2701 gggctttact gcagggaagg attgtcatgc ggtaattgaa acaaatcgcg ggcactggct
     2761 cggtcaggtg atttactcag gttgtgcgca ggagaatacc ggtgttcccg gcaatattat
     2821 ggggcatacc acccgacgag tgatccgcgc tcctgctgca ggcattatgc gatccaacgt
     2881 gaaattaggc gatctggtga aagagggcga tgtcattgcc tggattggtg agcatgaaat
     2941 taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac gacggactgg ccgtggtcgg
     3001 tggttttaaa attggtgata tcgatcctcg tggtgaaacg gctgatttca ccagcgtttc
     3061 tgataaagcc cgggcgattg gcggcggcgt acttgaggcg ttaatgatgt tgatgcatca
     3121 gggcgttaaa gcgacaaaag aagtgctgga agtggcttaa atgaaaatgg ccggatggta
     3181 ttacccatcc ggcctttaaa ttactgacaa atcaccgttc cggttttgcc ttcaatccct
     3241 tctttcgctt tgctcaatac ggtaatcagt gcttcacgac ctgcgcgaga gcgagcaaac
     3301 gaggcggcag cttccacttt aggcagcata gaacctttcg caaaatgacc ttcttcgata
     3361 aagcgttcag catcacttaa tgacaggcga tcgagccact gttcattctc ttttccgaag
     3421 ttaatggcca ctttttctac cgcagtcaga atgatcagca tatcggcatc gatcatttct
     3481 gctaaacggg cactggccca gtctttatcg ataaccgcgc tggcaccacg cagatggtta
     3541 ccttcacgaa taacagggat accaccaccg ccaacggtga tcaccacctg tccggcatct
     3601 accagagctt taaccgtttc tttttcaatg atatcaaccg gttttggcga agcaacgaca
     3661 cgacgatagc cgcgacccgc atcttctttc agagtgtaac cctgttttgt cagtagctct
     3721 gcttcttgct cggtaaagaa cgaaccaatc ggcttggtcg ggttgaggaa tgccggatcg
     3781 ttagcatcga cttcgacctg ggtaaccagc gttgctacag gtttattaat gccacgagaa
     3841 agcagttctt ctcttaaggc gttttgtaga tcgtaaccaa tataaccctg gcttaaggca
     3901 acacagacag acatcggcag cattggcgag tgcgcttcag tttttgcggc agcttcaaag
     3961 gcctggttaa tcatgcccac ctgtggcccg ttgccatgag tgacgatgac ctcatgtccc
     4021 tgggcaatta aatcaacaat tgcctgagaa gtaattttta ccgctttcat ttgtccggcc
     4081 aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa ttttcttact catttttcct
     4141 cactcatcat tcttgattgt gtatagatcc ctggcagtgg ttagagcacg ggagggacaa
     4201 acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc tgtaaaggtg ccgtcgcaga
     4261 aaatcgtttc accacgggat aaggttctga caatcgcgcc ctgacaggta aaaccctccc
     4321 atggcgagta gtcagcgttg tcgtggagat ggcgatgctg aatttgttgg ctctgacgtg
     4381 ggtctataat caccacgtcg ccatcggaac cgggcgctaa taatcctttt tgtggccaca
     4441 ggccaaacag cctggcgggc attgcactgg ttaactcaac aaaacgctcc ggtgagatac
     4501 gccccgtcat cacgccactg gagaacagca gttgcatgcg gttctccaca ccgggtaagc
     4561 catttgggca gcggctgaaa tcgcctttag aaatttgcag gcgttgagcc atcgagaagg
     4621 tgcagtgatc ggttgccacc acgtcaatcg caccatcgct gatgccacac cacagtttgt
     4681 cctgctcgcg tacgttacgc agcggtgggc taagaatgaa cttcatgcca tcttctgtat
     4741 cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt ttcaacccag actggctggt
     4801 gattcgcacg ggcaagacgc agataatcca gacctaagcc gttagacagg tgcacgatat
     4861 agagcggggc gttaccggca atttgtgcca ggttaatcat gcgggcgatg gcttccgctt
     4921 cgcattccag aggtcgactc aaggcatgat aacgcggcgc ggttaaccct gcggcgataa
     4981 actccgcccg cttgctggcg atagctgcgt cattttccgg gtgcacggtg gtcagcgcgc
     5041 cggactcatg caagcggcgt aatgcctgca acacttcatc atcgttgagt ttatattgat
     5101 aggttaaata gagtttaaaa ctgctcagtc cttcctcaac catcatcgga atttcgtcga
     5161 ggatagcgtg attaatatgt tgaatgacac cgtgaaagct gtagtcgatg actgctttgt
     5221 gggcggcata accgcgataa acttccagtt gatggcgtaa ccggcagcca tttgggccaa
     5281 atcccatatg gtcaataatg gttgttgtac cgccacacgc agctgcgcgg gtaccggtaa
     5341 aaaaatcatc acaactgcgc gcgatgccga catcaatatt gaaatgcgta tggacatcca
     5401 cgccgccagg gaaaacgtaa cagccagagg catcaatttc ttcgcaaggg agttgcggcg
     5461 aaatatcggt acccaactgg cgaacaatcc cgctttcaat cagcaaatcc tgcttcgcct
     5521 gtccatctgc attaacaaca atgccgtttt ttatcaatac gcgcatagca aactccaaaa
     5581 accctccggt gaaccggagg ggaataagtg aagagtaatt attcggttgc cagccagctt
     5641 aatgggattg cagcgtacat agcggcacag gttaccaggt gagatttcca ggttttttcg
     5701 tttggcgcgt gcgcttccgg ttctttaccg gggccaaaac cgatgaccgg aatgccgtgg
     5761 cgacccatga tggatacgcc attagtcgag aaggtccact tatcaacgac aggcgcttta
     5821 ccaaacagcc cttcataagc attcaccagt gctttaacgg tgaagtgatc ttcttccact
     5881 ttccaggtcg ggaagtagca ttcggttggg taaaccaggc cagtccagga cgggcggtcg
     5941 tagttgtaca tagaaacgac agcattcgct ttttgtactg caggcagggc acggatctcg
     6001 tccagcgcgc cttcccaggt ttcgccccag gtcagacgac ggtcgattga aactgcgcag
     6061 ctgtctgcga cagcgcaacg gcttggggag gtgaagaaaa tttcagaaac ggtgagggtg
     6121 cctttgccga ggaattcgtc ataacccaga cgttgggaga gttcttgtaa ttcgccaaga
     6181 atcggaccca ttttgaaaat ggcgttatca ccgcgttctg gtgccgaacc gtggcagcta
     6241 acaccctgaa catcaatacg aatttccata cgaccgcgtt gaccacggta tacctggcag
     6301 tcggttggtt cggtactgac cacaaattcc gggcgaatgc cggattgttc aataatgtac
     6361 tgccagcaca ggccgtcgca gtcttcttcc tgcacagtac cggtaaccag cagggtatat
     6421 tcatcttcca gaccgagatc tttaatgatt ttaccggcat ataccataga cgccatgccg
     6481 ccttcctggt cggaagtacc acgaccaccg atcagctcgt cggtttccat gccttcatac
     6541 ggatcgaagt cccagttttt gatgttgcca atgccgactg tatcgatatg agcgtccatt
     6601 gccaccagac gcgggccgtg gccgatataa ccgagaacgt tgcccattgg gtcgatttca
     6661 actttatcga agccgacttt ttccatctct tctttaatac gatgtactac gcgtttctcg
     6721 tcgcagcttt cactgggaat agcaaccata tcgcgcagga agcgagtcat gtctgcctgg
     6781 taatcttttg ctttttcaag aatcagtttg aatggaatat tcttagccat tatttttctc
     6841 cagatttttt actccccgtt ttgcagggag cagttccaat agggtgatta aggtgctaca
     6901 gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca cgtcggtgtc gccttcagtg
     6961 ctgataacca gcaccacggc atctttgttc agcgccagtt tttccatcag gctttgacgt
     7021 tgcgggtgat aatgaaccgc tgcgagaacg cccaaaccga cagcgccgga ttcaccggag
     7081 atgatgcgcg ggtcgttgcc gtacggatta cccagcacgc gcatacctaa tgcggcaacg
     7141 ctgtcctggc aggagatgaa ttgggtggcg cagttacgta ggatttccca gcccagcggg
     7201 ttaggttcgc cgcaggccag gcccgccatg atggtggcca tatcaccgcc aacgttgacg
     7261 atgtcgcctt tgacgccgga gcgataaata cagtcagctt tgtcaggttc aacaataatg
     7321 ctgtgcagat tttgcgggct atagacgtcg accagataac ccagcacacc accggccatt
     7381 gcaccgacac ctgcttgcag cagaacgtgt gtcggggtta cgcccatttc acgcatttgc
     7441 tcgacggctt catccgccag tgttgcgtag ccttgcatga tccaggttgg gattttggtg
     7501 taaccttccc atgccgtgtc ctgtaccact tcccagccgt gctgctgcgc gtgttgcatg
     7561 gtcaggcgaa cggtatcgtc atagttcata tccgtgacga tgcactcggc accgaggttt
     7621 agaatggcgt caacgcgttc ctgagcagaa cctttcggca tgtaaatcac cgcattctgt
     7681 ccgagttgct gagcggccca tgccacaccg cgcccgtggt tgccgtcggt ggtggtcgcg
     7741 aaagtcattt tttcgccaat ggcatttttc aggtgctcaa atgatagcgt ttcgatatca
     7801 agatgatatt tttcacacaa taattgagcg atggcgtatg cgccgccgag cattttgaag
     7861 gcgttcagac cgaagcgttt tgattcgtcc ttaacgagaa ttttctttac accaaacagg
     7921 tttgcgagat cgtccagcgc acaaagcggt gttgggcgat aaccagcaat tttctggtgg
     7981 aactggcgtg ccagttttgc ctggctttgc gaaaaaagcg gtgatgtttc gccgttgaaa
     8041 aatttgttat cggcgatatc aatctttaat gagaaaacgg acatatccta tccttttggc
     8101 ctctggaaat agagggccga ccagctgatc ggcccgaggg gttatttcac gcgttcttgc
     8161 gcttctttca gcagttgctc gagcagtgca cctggtttgg cgtatttacg ggacaggatc
     8221 atcgcggcga tgatataagg cttccagctg gcttctttgt aggtagcgat acggtatttt
     8281 tcgaatacgc cttcagtcac ttcaccttct ttgcaggata cgccgctgat atcagctggc
     8341 aggcagtgca tgtacagggc ttcgccatca cgggtcagct ccatcatctc ttcggtacaa
     8401 tgccagtctt tgtgctgtgc gttctgtgcc agacactgtt tttccagcgc tttcagtcct
     8461 tcgtgatcgt tcgcacgcag cagttcagta cgctcttcca tcactttgta aggtgcccag
     8521 gacttcggat aaacgatgtc tgcgtctttg aaggcttctt ccatgctggt gacctgacgg
     8581 aagctaccac cggaggcttt agcattgttt ttagcaactt caacgacatc cgggatcagg
     8641 tcgtagcctt ccggatgggc cagggtgaca tccataccga agcgggtcat cagaccgatg
     8701 atgccttgtg gtacagagag cggtttgcca tagcttgggg agtaggccca ggtcatggcg
     8761 atttttttac ctttcaggtt ttccagtgaa ccaaagtgtt cacgtaacca tgccagatca
     8821 gccattgatt gagtcgggtg gtcaatatcg cattgcaggt tcactaaagc cggacgctgc
     8881 ggcagcacac cctgcttgta accgtcatca agtgcagcgc caacttcacg catataggcg
     8941 ttgcctgcgc ccagatacat atcgtcgcga ataccgatag cgtcagcgca gaaggagatc
     9001 atattggcgg tttcacgcac ggtttcgccg tgagcgattt gtgatttacc ttcgtcaaga
     9061 tcttgttgtg caaggccgag caggttaagc gcggaagcat aagagaagcg ggtacgggtg
     9121 gaattgtcgc ggaatacgga aatacctaat ccactattaa agactttggt tgagatgttt
     9181 tcagcacgca gtgcttttaa cgcggcggca acgtccagta cttgtttcag ttcgtctggc
     9241 gtctgttccc acgttaacaa aaaatctttt tcatgcaggt gagaggtcag cgaattgata
     9301 tccttaatca gctcattaac agttttcatc ataaagtccc gatagtggaa tgacaggcaa
     9361 agtggtttat ttcactgtga cgtgccaaag tgattggtgt tttcacagct atacctcgga
     9421 ctaacaataa gtgtgccaga attatccacg cggctgcgaa aggctaattt gacggataat
     9481 gtgtggggtt gatcacatat tgatgccagt ttccggttgt ttgtaaaata cgaaaacgtt
     9541 tggctttttt tattacgtta tttatttgct gctaagtatc agaaataaga taaagaggca
     9601 ggctatctct cttcagtttg ata
//
LOCUS       DACADA010000165         9405 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.167, whole genome shotgun sequence.
ACCESSION   DACADA010000165 DACADA010000000
VERSION     DACADA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.167"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            85..1149
                     /gene="ulaG"
                     /locus_tag="ISB17_003988"
     CDS             85..1149
                     /gene="ulaG"
                     /locus_tag="ISB17_003988"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP1981864.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1257..2012
                     /gene="ulaR"
                     /locus_tag="ISB17_003989"
     CDS             1257..2012
                     /gene="ulaR"
                     /locus_tag="ISB17_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAP1981865.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2009..2758)
                     /gene="yjfP"
                     /locus_tag="ISB17_003990"
     CDS             complement(2009..2758)
                     /gene="yjfP"
                     /locus_tag="ISB17_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1981866.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2940..3269
                     /gene="bsmA"
                     /locus_tag="ISB17_003991"
     CDS             2940..3269
                     /gene="bsmA"
                     /locus_tag="ISB17_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAP1981867.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3418..3693
                     /gene="yjfN"
                     /locus_tag="ISB17_003992"
     CDS             3418..3693
                     /gene="yjfN"
                     /locus_tag="ISB17_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAP1981868.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3810..5435)
                     /locus_tag="ISB17_003993"
     CDS             complement(3810..5435)
                     /locus_tag="ISB17_003993"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAP1981869.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5519..6682)
                     /locus_tag="ISB17_003994"
     CDS             complement(5519..6682)
                     /locus_tag="ISB17_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP1981870.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6685..7323)
                     /locus_tag="ISB17_003995"
     CDS             complement(6685..7323)
                     /locus_tag="ISB17_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAP1981871.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7333..7731)
                     /locus_tag="ISB17_003996"
     CDS             complement(7333..7731)
                     /locus_tag="ISB17_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAP1981872.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7749..8408)
                     /locus_tag="ISB17_003997"
     CDS             complement(7749..8408)
                     /locus_tag="ISB17_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HAP1981873.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(8458..9156)
                     /locus_tag="ISB17_003998"
     CDS             complement(8458..9156)
                     /locus_tag="ISB17_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAP1981874.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9175..>9405)
                     /locus_tag="ISB17_003999"
     CDS             complement(9175..>9405)
                     /locus_tag="ISB17_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAP1981875.1"
                     /translation="PVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGAL
                     SLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2442 a         2502 c         2246 g         2215 t
ORIGIN      
        1 tgccttcctg aaaaaatagt gtcaggatta atcaaaacca atcacatatt gattcgaatt
       61 gaactatcac gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg tgagtcctgg
      121 atcctgagca ctttcccgga gtggggtagc tggctgaatg aagaaattga gcaagagcag
      181 gtcgcaccag gcactttcgc aatgtggtgg ctcggctgta ccggaatctg gttgaaatca
      241 gaaggcggtg ccaacgtttg tgttgatttt tggtgcggta ctggcaaaca aagccacggt
      301 aacccgttaa tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag
      361 ccaaacctgc gcaccacccc gtttgttctt gatccgtttg cgattcgcca gatcgacgcg
      421 gtactggcga ctcacgatca caacgatcat atcgacgtta acgtcgctgc tgccgtgatg
      481 cagaactgtg ctgatgacgt accgttcatt ggaccgaaaa cctgcgttga tttgtggatt
      541 ggctggggcg taccgaaaga gcgttgcatc gtcgttaaac cgggcgacgt agtgaaggta
      601 aaagacattg aaattcatgc acttgatgct ttcgaccgca cggcactgat cacgctgcca
      661 gccgatcaaa aagcggctgg cgtactgccg gacggcatgg acgatcgcgc ggtaaactac
      721 ctgttcaaaa cgccgggcgg ttccctgtat cacagcggcg attcccacta ctccaactat
      781 tatgcaaaac atggtaacga gcatcagatc gacgtggctt taggatcgta cggagagaac
      841 ccacgtggta tcaccgacaa aatgaccagt gctgatatcc tgcgtatggg cgaagcgctg
      901 aatgcgaaag tagtgatccc gttccaccac gatatctggt caaacttcca ggccgatccg
      961 caagagatcc gcgtgctgtg ggagatgaaa aaagatcgcc tgaagtatgg cttcaagccg
     1021 tttatctggc aggtcggcgg caaatttacc tggccgctgg ataaagacaa cttcgagtac
     1081 cactatccgc gtggtttcga tgattgcttc actattgaac cagatctgcc gttcaagtcg
     1141 ttcctgtaat ctgtcgcttg taccggatag cgtggagatg ctcgcgctat ccggcatagt
     1201 atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa tagctcatga
     1261 ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc tttgtgaccg
     1321 ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcacgacgc gatatcaata
     1381 aacttgacga aagcggcaaa ctgaaaaaag tgcgcaatgg cgcagaagct attacccaac
     1441 agcgcccgcg ctggacgccg atgaatctgc atcaagcgca gaatcacgat gaaaaagtac
     1501 gtattgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc atcaactgcg
     1561 gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta
     1621 attatctacc gctggcaaat tacctgatcg atcaggagca tgacagcgtg atcattatgg
     1681 gcggacagta caacaaaagt cagtccatca ctttaagccc gcaaggcagc gaaaacagtc
     1741 tctatgccgg gcactggatg tttaccagcg gcaaaggact gacagcagaa gggttgtata
     1801 aaaccgatat gctgacggca atggcagagc agaagatgct gagcgtggta gggaaactgg
     1861 tggtactggt tgatagcagt aagattggcg aacgcgcggg aatgcttttt agccgtgccg
     1921 atcaaatcga tatgctgatc accggcaaaa atgctaaccc ggaaatcctg caacaactgg
     1981 aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag
     2041 catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca
     2101 gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca
     2161 cgacgtcatc gtcaaggcca tgccataaga gcagcggtct gttccccaac tctgtcagat
     2221 ggtttgtcgc ttcccactct gccagcggcg cgacaatgtt attgaattca ttctgctgtg
     2281 ctgccgtttc aggtatcagc ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg
     2341 atcccatcat gctggcggta cattttaccg tggggtggcg agcggtaatt cccagtgccg
     2401 tcatcgcgcc catcgacgcg ccaccgactg ccagacggtc atcacgcagc cagttttctt
     2461 cggctattgc cgcacgtaag gtagtgaatt cctgcatact ttgtagcaag atttgccaga
     2521 attgatttaa ccgccgcgct gcgtcgccac taaaacggct accgtgatcg ggcgcatccg
     2581 gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccagac
     2641 tggacgaagt aaagccgtga taaaaaatca cacacggtaa cggggtatct ttttgcccga
     2701 caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct
     2761 gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg
     2821 tattagttca taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg
     2881 cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta
     2941 tggttagcag gaaacgtaat agcattattt accggtttgc cagtttatta ttggtgttga
     3001 tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacggacc
     3061 atccgcaaga gattcgccgt gaccagacgc aagggttaca gcgaataggt agcgtaagca
     3121 cgatggttcg aggttctccg gatgacgcat tagcagaaat cagagcgaaa gctgtcgctg
     3181 caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt
     3241 attcacaagc cattttgtac cgtaaataaa tcagacaact aacatagtca gctttacatt
     3301 gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa gctcgggcgt
     3361 ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg
     3421 aaacaattac ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca
     3481 cagtctgcag aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc
     3541 tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc
     3601 gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat
     3661 aactggcgcg tacaggcgat actgtatgcg tgagtgtcat ctacagcaaa tgaacaatgc
     3721 cggatgcggc gcgagcgcct tgtccggcct acaattttaa aatatttcat ttagttcatc
     3781 gaaaccagcg tcgcatcagt cgtatacgct tacaaacaca ctcctcccgt cgcccgcagc
     3841 aataaatcat tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc
     3901 ttacaccacg tctgcgccat tggcggagag gcatatttca acatttgcgc accgcagccc
     3961 agcaggaata gctgatgagt aatctctcgc cccaattctt cagctggctt acgcagctgc
     4021 tgctgtaaac gacgaaccgc gcgatcaaaa tagcgatcct gccctttcac ttccacaaat
     4081 gcttccgaca ataagtcgta tacgcccgct tgcttattga gaacgcgcag cacatccagg
     4141 cacataatat tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc
     4201 ggcagctcgc tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca
     4261 aacggaatgc cacgtttgca gatcacaaat ttcgccgcag gcgtaaataa acgcgcccac
     4321 agagcttctt tggcatcggc gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc
     4381 gtttgccctt caagctgaag tgccatgcga cttaagacat gacgcataag gggctgttgg
     4441 atcaatggat taccaaaaac atggcgttga tgtgcatgat aaatcgccag cgaaaatgca
     4501 cggcgcatca tggcatggct acccagggcg caatcaaaac gcgtcatccc acccattttc
     4561 aggatcagac gaattccttc cccttcctgc cccaacaacc agccaatggc atcctgaaat
     4621 tccacttcgc agctggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga
     4681 atcgcgttgc gttgcccgtc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc
     4741 gcggtctgtg ccagcaccag atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc
     4801 cccaccagcc gataagaacc atcgtccaga cgctctgcgc gggtggtgtt gctcatgaca
     4861 tcggaaccgc cctgcttttc cgtcattccc atgccgatca acaagccacg tttttgccca
     4921 cctggcaata agtgagaatc gtagcgatcg ctcaacagcg gcgtggtcca gtcctgaaac
     4981 ggcgcgggta acatctgtaa caacaatggc gtggcggcaa aggtcatcgt tatcggacat
     5041 aacgacccag cctcaacctg cgcatgtaac ataaaacgcg ccgcgcgcgc cacaaatgcg
     5101 ccggagcgag cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc
     5161 atcagcaggt gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg
     5221 tagcgcaata attcaggcgg attcacattc gccagccgcc ccagttcaag ggattcagcc
     5281 gttcctaact gctgaccaat actggcgaga aaatcgctat cccagccagc accttcacgc
     5341 gttaccgctt cgcaaagcgc gccatcagac aggtataagt tgctgttatt taatggtata
     5401 ggttgattaa aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt
     5461 caccattagt atggtcactg ccatggattc atgacagaaa gagattgcta aaacattctt
     5521 atccagcaat gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagc gtgttatctt
     5581 cacggatccc cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc
     5641 tatcgccaaa tcgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat
     5701 caccatcagc gtggtcaaca acgttattct gaccgtcgaa aatggtgacg tttccgcctt
     5761 cgcgcgagta aatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcac
     5821 catcgaacca ggatgcaaga aggttaggat gaccagggaa gaagcgccaa agcagcggca
     5881 ttagcccttt attactcaag atacttttcc ataacggctc cacccattgc tcacgacgct
     5941 tgcgcagcag cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg
     6001 cacgctggat gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagct
     6061 cttcaatgta gataaaccga gactcctgcc ctgcctgctc ggcgcagtcc tgcaagtaca
     6121 gcacggtact cctgtcttca tcggtatcct gacagcagca aaaataaaac ggctcccggc
     6181 tataaagctc gctaaagcgt gaaatcaggc gctcctgaat ggcattgtac tgatcggcat
     6241 cgcgcggaat aacgccgctg cgccgggcat cttccagcca caaccactgg aaataagccg
     6301 actcgtacaa tgaagttggc gtatcggcgt tgtactccag caacttaacc ggtgcattac
     6361 cacaccaggc aaaatccata cggccataca acgaaggatc gcgtgcgcgc cagctttcgg
     6421 cgatcacatc ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcatctttaa
     6481 ccgcacgatc caccacctcc aggcacatct gatgcagttc cgcagtcggt ttttcgatct
     6541 gctcttcaat ctggcgcaga gtaaagcggt aagcccgact ctcatcccaa tagatttcat
     6601 tgtcgatgat atgaaagtca aatccgttat cggcggcaat ccggtccaga tcccgccgca
     6661 caggaacgtt gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg
     6721 accatagccg ccgcgagaaa cggttgacgc ttttttggtg gtaaagccgc ccgaagagta
     6781 agactctttt ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgcgccagac
     6841 ggggcgggaa gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt
     6901 acgaataacc cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt
     6961 gtcgtagtaa caattttcat acttagactg acaattctgc tgagtcatat tcttcggaac
     7021 atcggcataa aatgccgctt tggcgttgtt ccagccatga gcacagatat ctgaattatt
     7081 accatcatca atacaatcct gcacagtcgc gtaaaacgtt ccgtcgccgt cgttatcgac
     7141 atcgccacta tcgccacaac ctttcaatac gaaaaaagcg gcaccgccca ttagcgccaa
     7201 cgttaagtat ttttgtgcgt agcgattgcg gcgcggctca aaaggattat gcggtctacc
     7261 aatgcgactg attgccccat gtccgatatt tttgttattt ctggatttgc gttttctggc
     7321 catgatcgtc cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc
     7381 ggttcccata aacattccgg ctgcggtatt gtgattaata attttttcgc ttaatgcggg
     7441 catataaagc ctgacgccag caaaaacgag taattgaatc accagagcaa tcccgcccca
     7501 tgcgaaatag tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta
     7561 ccccaacaat gttccgctga acgccagtgc cgccgcggtg ttattgtttt tgattaactg
     7621 ccattcgttg tgcggtgtaa ttttggagta gataaacagg aaaataatca ccatcgccac
     7681 gccaataaaa aaataggcac taaaagcaag aagtgaatcc agtatatgca ttgtaaacgt
     7741 ccttgtaatt aaccaataat atgaagtgat gtcagcggga tatcgatacc cagcgcgcgc
     7801 gaaaataacc actccggctc gccgagatcg ttaaaagatt cctcaccatt taacagcaaa
     7861 tattcgtaag tatctccggt cacctgccgt tggtagccca tgctaaaatt atgcacttcc
     7921 catttggcat ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc
     7981 ggttcttcgc tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc
     8041 cccatcgctt tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc
     8101 tcttcataga caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccagtggta
     8161 ttgatttgca ggaactcatc gcccgaagtg tagtaacgga aaatctggct acccccgccc
     8221 agatcgatgt ggctgacagc ggctaccgta aattcctcac ccggcagcgc aatcagcaat
     8281 tcatcttcca gcaaacgaaa cgataatgtg tcgagcgtaa agccactatt gagatgcaga
     8341 cccagcggac cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa
     8401 ccagacatat tttatcctta aaatagttac cagccttgca gtgaggctgg caaaaaatta
     8461 ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc
     8521 gccaattcca gcttccgcca gcttttcatc gagatcgcga ccgtccgcga ctttttccaa
     8581 ctgtgcggca gcatccaggc gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa
     8641 ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg gtagaggtcg ttacagcctg
     8701 ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc
     8761 gatacgctgc gctgtcgctg taactgcttt ttccacgcca tcacgagaga cttccagatt
     8821 cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac
     8881 ttcgttaatc aacgacggat taacgttctt gcttaacgct tccagcgcac gcgcttccag
     8941 actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca cccgtgccag
     9001 cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg
     9061 ttccagcatg cgcacgccct gggtttcttc aatggactct tccgcctggg agataaacga
     9121 tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg
     9181 tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc
     9241 aagcaggata tcttcaagag aagatttaag cgataacgca ccgaaaacaa tgtaatactc
     9301 ttcctgttgc acgctggaga tccctaccga tgacaacggc atcatcttct gatttcttaa
     9361 taagaaggta ttaaattcat ctggattgct gatactactt accgg
//
LOCUS       DACADA010000166         9374 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.168, whole genome shotgun sequence.
ACCESSION   DACADA010000166 DACADA010000000
VERSION     DACADA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.168"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            241..1539
                     /gene="kgtP"
                     /locus_tag="ISB17_004000"
     CDS             241..1539
                     /gene="kgtP"
                     /locus_tag="ISB17_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAP1981876.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1536..1859)
                     /locus_tag="ISB17_004001"
     CDS             complement(1536..1859)
                     /locus_tag="ISB17_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAP1981877.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="ISB17_004002"
     CDS             complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="ISB17_004002"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAP1981878.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3374..6034)
                     /gene="pat"
                     /locus_tag="ISB17_004003"
     CDS             complement(3374..6034)
                     /gene="pat"
                     /locus_tag="ISB17_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAP1981879.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6066..6764)
                     /locus_tag="ISB17_004004"
     CDS             complement(6066..6764)
                     /locus_tag="ISB17_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAP1981880.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="ISB17_004005"
     CDS             complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="ISB17_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAP1981881.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7459..8496
                     /locus_tag="ISB17_004006"
     CDS             7459..8496
                     /locus_tag="ISB17_004006"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP1981882.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8544..9233)
                     /gene="ung"
                     /locus_tag="ISB17_004007"
     CDS             complement(8544..9233)
                     /gene="ung"
                     /locus_tag="ISB17_004007"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP1981883.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
BASE COUNT         2293 a         2382 c         2427 g         2272 t
ORIGIN      
        1 ttatactccc acataagcca gattcaacag cgaatacgtc ttccccaaat tgcccacttc
       61 catacttcct ccttaccaga aatctatcct taagctcctt aataaccatt ttcctgctaa
      121 ctaaattcat ggttaaggtt gtataatgat gcgcaacaaa tgtataacat tttctttacc
      181 aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag acataatggc
      241 atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac tcgtcgccgc
      301 atttgggcaa ttgttggggc ctcttcaggt aatctggtcg agtggttcga tttctatgtc
      361 tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa cacgacgact
      421 caactactgc aaacagcagg tgtttttgct gcaggatttc tgatgcgccc aataggcggt
      481 tggctgtttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg
      541 tgtatgatgt gtttcggctc actggttatc gcctgtttgc caggctacga aacaatcggt
      601 acctgggctc cggcattatt gcttctcgcg cgtttattcc agggattatc cgttggcgga
      661 gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg caaaggtttt
      721 tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct actggttgtc
      781 gtggttttac aacacaccat ggaagacgct gtactcagag agtggggatg gcgtattcct
      841 ttcgcgttag gagctgtgtt agctgttgtg gcgttgcggt tacgtcgtca gttagatgaa
      901 acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg attatggcgc
      961 aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct ttgtttctat
     1021 accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca tgccaacgtg
     1081 gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc aattattggc
     1141 gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc tctggcaacc
     1201 atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta tgccgctttt
     1261 ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag tggaatactg
     1321 aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc atatgcgttc
     1381 gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa atcaatagga
     1441 atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt tctggtttca
     1501 ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag ttgccagacg
     1561 gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct ccagccgctc
     1621 ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga gaacatcaat
     1681 ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc atttccggcg
     1741 gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct ccagttatcg
     1801 ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag aatacgcaaa
     1861 acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag gatgcggcta
     1921 attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac cttcaccgga
     1981 taatcggcaa tactttgcag atcgcggtag tgcttaacga tggtggtatg ctcgcggatc
     2041 agatccagtt ctttctctcg ctgtggcgca agctccagtt gcggatcgtg gatcaaaatg
     2101 gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt gatcaaaatc
     2161 cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc tttccataac
     2221 cgaactacta actggtcagt gttgacgtaa tactgtaaac gactcaggaa acgacgcaga
     2281 ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc ttccggaatg
     2341 tagaagtcat tcgcggtttt atcaccaaca ataatttcga cctttttccc ttcgcgcagc
     2401 aactggataa tattgcgcac aaggattgct ggtaggttga agtaaggtgt gcagatggtt
     2461 agtttctgct cggcacaagg cataagatgg aaaatagtct tgttcaacag actcgatttc
     2521 cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga
     2581 taagcggcat cacgcagctc ctggcggaac aggcgaatgt cgttcttgat ttccgggctt
     2641 tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat attctgtgta
     2701 acccattcaa acataatgtc tgacatctta cggttacgga tcagatgata acggtcgtag
     2761 cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct ataaagtacg
     2821 ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg ggtattgatt
     2881 ggaacgccat aaaccggaac atttacgccc ggattttcct gcgccatgcg gcagtaccag
     2941 tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg
     3001 accagcaccc gcacatccag ttccggacgc tgccttttag cctcatacaa cgcgttcaga
     3061 atgcctttgc caccgtcatc ctgttcgaga tacagggcaa caatgcaaat gcgctgcttc
     3121 gcgctggcta ttttttccag caacgtctcc cggaagtcgg cgggagcgta aaagaaatcg
     3181 acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg atgtttatta
     3241 cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag ggtcctctgt
     3301 gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt ggtcaacatt
     3361 tccagtacct tactcatgat tcctcgcgct gggcaagact tagcgtaagc ccaacgatcc
     3421 cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggccagcgcc accatgccac
     3481 gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc gtataggtaa
     3541 tcaacttttc cattaagcgt cgacctaagc ctaacccttt aagatccgag cgaaccagta
     3601 cggcaaattc ggcatcgatg ttatcagggt cggagatcgc acgtgtgacg ccgaggatct
     3661 cttccgtttg atcaattcgt cgtaccgcta caaatgccat ttcccgatcg tagtcgatct
     3721 gcgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag tagcggtaat
     3781 aaagatcttc tttggtgact cgcgaaatga attgctgaag ctgtggctca tcttctggca
     3841 aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct tccagctgat
     3901 gcggataagg gcgcacagcc agtcggctct cgttatcacc ttcaaacggc gcgatatcca
     3961 gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga atatccagac
     4021 gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc tggctcaagc
     4081 ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt ttgatcccct
     4141 gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg acggcttgat
     4201 cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg aaaaccggat
     4261 cgtgctcaac cacaacccgc aactcctgag cgccagcacg gttagccata ctttgcacca
     4321 acaggccgtg gacccgcgcc tgcggccagg ccatttttac gcgatcgaaa atagcgtttg
     4381 ccgcttgctg gacttcattg gctgtacgta ggtagagcat gacgccctga acttccgatt
     4441 tatgtggaat atccggcgag cgcaatttca gtgccaccgg atagccaatc tgttcggcaa
     4501 tatgtaccgc ttcggtgcta tcactggcaa tccaggtagg gagagtattc ataccatacg
     4561 cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg gcaatcgcct
     4621 gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc aacgccggcg
     4681 tttcgcgtag ttgcttctga ttacgccggt actcgaccat atgcataaaa gcagtgatgg
     4741 ttccttccgg agtacggtag gtcggcagcc cggcttcgct gaataaacgt cgtgcctctt
     4801 gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg cggggatgat
     4861 gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg
     4921 aatgaataac catcagcgca tcaaaatcct ggctgtggag cagaatgtcc agcgttttga
     4981 cataatgctc gctgctggcg tcatcgcgca gatcgagcgg gttagagatt gccacatgcc
     5041 ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc agcttgccat
     5101 tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg ctgataatca
     5161 tcaaccggtc gccacgcagc gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt
     5221 cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agctgcatcc cacgccggat
     5281 ccattcctgc cgtggtgttc aacagacgtt gagccgccgg gctacgtccg cttttaatca
     5341 ccaaaatcgg tttattgcgc gaggcactac gggctgccga aacaaagcgt cgcgcgtcgc
     5401 ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgcgcc agatagtcga
     5461 gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag gaaaagccca
     5521 tctcacgctg ttgcgcccag tcgaggatgg tgttggagac agcagccgat tgcgaaataa
     5581 acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga ccttgccagg
     5641 gagccagtaa gcccagactg tttggtccta gcaggcgcat gttatggcgc agggcgcagg
     5701 cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt ttacagcctt
     5761 tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat aaaaccgcaa
     5821 ggtcgggtgt aaagggcaag ttggcaatat ccggccaggc caacacaccc aacaccgctt
     5881 tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc aggttacgca
     5941 tcatcaggta acctgcgcga ttgggtttca tcgatgcgcc aattaccgca atcgattttg
     6001 gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga agtgaccgaa
     6061 tgattttaaa cgctttctaa ctgttctgct gtgatgcttc ccggatgttg cgtttttcct
     6121 gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgtcgc cgccctggta
     6181 tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg gccttcagcc
     6241 tgggcttcac gcaggcgata ggcagaaagc cgagaaagat cgacggaaat gacgggaaga
     6301 ttatccagat acggactttt acgaaacatc ttgcgagctt ctggccaggt accatcgagc
     6361 atgataaata gcggaggctt atcggcaggt ggtgtgaaga tcacttcccg ttgctcatca
     6421 gcatacgaag cgggaaagac caccattggc tgatagtctg ggttttgcac cagatccagc
     6481 aaatcttgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg caaaatatca
     6541 gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat cagcaaacag
     6601 aagtgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt ctctggcaaa
     6661 agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc
     6721 gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg tcataagaga gcgtcgaaaa
     6781 aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa gattaaagag
     6841 attcgttcag ccagctgtcg aacggcgctt tcggtactgc gccattaagc atgtcgacaa
     6901 cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag cgactgctca
     6961 attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta cgctcttgcg
     7021 cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt gcccagaagt
     7081 cgatcaccac aggtagatca tctttcagca atttgtcgag cgtttcaccg gtcgcattaa
     7141 tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc
     7201 gatcgtcggg aatgcgattg atggcctgac aatgggtaca aacggtattc ataactaacc
     7261 tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat atgttacata
     7321 ttatcgggga cattgttcca gacaacaatc tgttttattc aatgggtatg atagacacag
     7381 gctttggaac gaaatcatgg cgaaccgcgc gcacttcggg taatctgcgc gcttcgcgca
     7441 gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg
     7501 atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg taccgggaaa
     7561 gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac
     7621 aaacagagtc agcaccgtga ccgcaagtgg gaagattcgc cgtggcgcac ggtttcccgc
     7681 gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg taaaagtttt
     7741 attgatccgg aagttttgcg tcgtcagcgt gcggaagaaa cccgcgtcta cggcgaaaac
     7801 gcctgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg gtttatccag
     7861 agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg caaagcgtac
     7921 catgtggtgg atgaagcgga actgacaaaa gcgtcaggca cggaacatca cggcggcgtt
     7981 tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag ccaggcaggc
     8041 gcgcaggatt gtgtactggc cctggaaaac gaatctaacc cgcataacct gggtggcatg
     8101 atgcgtagct gtgcgcactt cggcgtgaaa ggtgttgtgg tgcaggatgc agcactgctg
     8161 gagtctgggg cggctatccg taccgcagaa ggcggcgcag agcacgttca gccgattact
     8221 ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg cgggttacac cgtagtgaca
     8281 acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa aatggtactg
     8341 gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa cgatctgcgc
     8401 gtgaagattg atggtactgg caacgttgcc gggctgaata tctctgtcgc aaccggcgtt
     8461 cttcttggcg aatggtggcg tcagaataaa gcctgataaa tcagccgggt ggcaactctg
     8521 ccatccggca ttttcccgca aatttactca ctctctgccg gtaatactgg catccagtca
     8581 atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg gttgcagcca
     8641 aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac atgatggcgt
     8701 tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa aaacaccacg
     8761 ccttcgcgat gctgatttat gaggctgatg actttatcag taaacgtctc ccagccgagg
     8821 ctggcgtggg aatgcgcctg accagcgcga accgtcaaga cggtattgag cagcagaaca
     8881 ccctgacgcg cccagctttc aagataacca tgattagggc gggtgaaacc gggaatagta
     8941 ttttccagct ctttatacat attcagcagt gacggtggtg tcgcaatgcc ggggcgaacg
     9001 gaaaatgcca gaccatgcgc ctgtcctggt ccgtgataag gatcctgacc gagaatcacc
     9061 actttaacgt cacccaactc cgtaaagcgg aacgcgttaa agacatcttt ttgtggtgga
     9121 tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt aagaaaatag
     9181 ggttgctgct tctcttcagc cagcacatca tgccaggtta attcgttagc catctcgctc
     9241 tcctgcgaat ctttaatccg cctagcttaa ctgcttcttt ctacagaaca aaatcatgct
     9301 cattttatag acaaaaagac atgcaaaaaa atgtaaattg gtaaaatttc acatctgcat
     9361 aagttgatgt aaaa
//
LOCUS       DACADA010000167         9265 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.169, whole genome shotgun sequence.
ACCESSION   DACADA010000167 DACADA010000000
VERSION     DACADA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.169"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            286..1626
                     /locus_tag="ISB17_004008"
     CDS             286..1626
                     /locus_tag="ISB17_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP1981884.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1704..3350
                     /locus_tag="ISB17_004009"
     CDS             1704..3350
                     /locus_tag="ISB17_004009"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP1981885.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3473..4123
                     /locus_tag="ISB17_004010"
     CDS             3473..4123
                     /locus_tag="ISB17_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAP1981886.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWLAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4120..5475)
                     /gene="melA"
                     /locus_tag="ISB17_004011"
     CDS             complement(4120..5475)
                     /gene="melA"
                     /locus_tag="ISB17_004011"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP1981887.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEDYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5758..6666
                     /gene="melR"
                     /locus_tag="ISB17_004012"
     CDS             5758..6666
                     /gene="melR"
                     /locus_tag="ISB17_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAP1981888.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAILNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6865..9132
                     /gene="adiA"
                     /locus_tag="ISB17_004013"
     CDS             6865..9132
                     /gene="adiA"
                     /locus_tag="ISB17_004013"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAP1981889.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2294 a         2488 c         2290 g         2193 t
ORIGIN      
        1 gaccgataaa tatcaataag ataacagcaa acaaaacatt aacatctgcg ctgtacaaac
       61 tataaaccca tcgccagaga gtctttctct ctgaaaaagc cgcttatcac agtgcataaa
      121 tttgccgctg ctttaatcag ccaatattca ctgtgaggta tttgctcaag ccggtaacga
      181 ccaaacggat atttagtcag gctctgaaaa cagttcatac aaaacagaac gtgactgtga
      241 tctattcagc aaaaatttaa ataggattat cgcgagggtt cacacatgtt atttactatc
      301 caacttatca taatactgat atgtctgttt tatggtgcca gaaagggtgg tatcgcgctg
      361 ggtttattag gcggtatcgg tctggtcatt ctggtcttcg tcttccacct tcagccaggt
      421 aaaccaccgg ttgatgtcat gctggttatc attgcggtgg tggcggcatc ggcgaccttg
      481 caagcttcgg gcggtcttga tgtcatgctg caaattgccg agaagctgct gcgtcgcaac
      541 ccgaaatatg tctcaattgt cgcgccgttt gtgacctgta cgctgaccat tctttgcggt
      601 acgggtcatg tggtttacac cattctgccg atcatctacg acgttgctat taagaacaac
      661 atccgtccgg aacgtccgat ggcggcaagt tctatcggtg cacagatggg gattatcgcc
      721 agtccggtgt cggttgcggt cgtatctctg gttgcgatgc tgggtaatgt cacctttgat
      781 ggtcgccatc ttgagttcct cgacctgctg gcaatcacca ttccatcgac gttaatcggt
      841 atcctggcga tcggtatctt cagctggttc cgcggtaaag atctggataa agacgaagag
      901 ttccagaaat tcatctccgt accggaaaac cgtgagtatg tttacggtga taccgcgacg
      961 ctgctggata aaaaactgcc gaaaagcaac tggctggcaa tgtggatctt cctcggggca
     1021 atcgctgtag tcgcccttct tggtgctgat tcggacctgc gtccatcctt cggcggcaaa
     1081 ccgctgtcga tggtactggt tattcagatg tttatgctgc tgaccggggc gctgattatt
     1141 atcctgacca aaaccaatcc cgcgtctatc tcaaaaaacg aagtcttccg ttccggtatg
     1201 atcgccatcg tggcggtgta cggtatcgca tggatggctg aaaccatgtt cggtgcgcat
     1261 atgtctgaaa ttcagggcgt actgggtgaa atggtgaaag agtatccgtg ggcctatgcc
     1321 attgttctgc tgctggtttc caagtttgta aactctcagg ctgcggcgct ggcggcgatt
     1381 gttccggtcg cgctggcgat cggcgttgat ccggcataca tcgtggcttc agcaccggct
     1441 tgctacggtt attacatcct gccgacttat ccgagcgatc tggcagcgat tcagtttgac
     1501 cgttccggca ccacccacat cggtcgcttc gtcatcaacc acagctttat tctgccgggg
     1561 ttgattggtg tgagcgtatc gtgcgtcttc ggctggatct tcgccgcgat gtacgggttc
     1621 ttataaatgc actttgcgtg ccgcccgtaa ctacgcggca cgccattttc gaataacaaa
     1681 tacagagtta caggctggaa gctatgtcaa acaaaccctt tatctaccag gcacctttcc
     1741 cgatggggaa agacaatacc gaatattatc tactcacttc cgattacgtt agcgttgccg
     1801 acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc cctgaccctg ctggcgcagc
     1861 aagcctttca cgatgcttct ttcatgctcc gcccggcaca ccagaaacag gttgcggcta
     1921 ttcttcacga tccagaagcc agcgaaaacg acaagtacgt ggcgctgcaa ttcttaagaa
     1981 actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca ggataccggc accgcgatca
     2041 tcgtcggtaa aaaaggtcag cgcgtgtgga ccggtggcgg tgatgaagaa gcactgtcga
     2101 aaggcgtcta taacacctat atcgaagata acctgcgcta ttcacagaac gcgccgctgg
     2161 acatgtataa agaggtcaac accggcacga acctgcctgc gcaaatcgac ctgtacgcgg
     2221 tagatggcga tgagtacaaa ttcctctgtg tcgctaaagg cggcggctct gccaacaaaa
     2281 cgtatctcta ccaggaaacc aaagccctgc tgacgcccgg caaactgaaa aacttcctcg
     2341 tcgagaaaat gcgtaccctc ggtactgcag cctgcccgcc gtaccatatc gcgtttgtga
     2401 ttggcggtac gtctgcggaa accaacctga aaaccgtcaa gttagcaagc gctcactatt
     2461 acgatgaact gccgacggaa gggaacgaac atggtcaggc gttccgcgat atccagctgg
     2521 aacaggaact gctggaagag gcacagaaac tcggtcttgg cgcgcagttt ggcggtaaat
     2581 acttcgcgca cgacatccgc gttatccgtc tgccacgtca cggcgcatcc tgcccggtag
     2641 gcatgggagt ctcctgctcc gctgaccgta acattaaagc gaaaatcaac cgcgaaggta
     2701 tctggatcga aaaactggaa cacaacccag gccagtacat tccacaagaa ctgcgtcagg
     2761 caggtgaagg cgaagcagtg aaagtcgacc ttaaccgccc gatgaaagag atccttgccc
     2821 agctttcgca atacccggta tccacccgct tgtcgctcac cggcaccatt atcgtgggtc
     2881 gcgatattgc acacgccaag ctgaaagagc tgattgacgc cggtaaagaa ctgccgcagt
     2941 acatcaaaga tcacccgatc tactacgcgg gtccggcgaa aacgcctgcc ggttatccat
     3001 caggttcact tggcccaacc accgcaggtc gtatggactc ctacgtggat ctgctgcaat
     3061 cccacggcgg cagcatgatc atgctggcga aaggtaaccg cagtcagcag gttaccgacg
     3121 cgtgccataa acacggcggc ttctacctcg gtagtatcgg cggtccggcg gcagtactgg
     3181 cgcagcagag catcaagcat ctggaatgcg tcgcttatcc ggagctgggt atggaagcta
     3241 tctggaaaat cgaagtagaa gatttcccgg cgtttatcct ggtcgatgac aaaggtaacg
     3301 acttcttcca gcagatcgtc aacaaacagt gcgcgaactg cactaagtaa cctcttcggc
     3361 ccagcgcctg gcagcatgct gccaggtgat ccccctgggc cacctctttt gcgattgtaa
     3421 tttcacgctt gctggtgaat agtcagtatt ttccctgact tgcgaactca ccatgacccg
     3481 cacactcaag ccgttaattc ttaacactgg cgcactggcg ctaacgttaa tcctgattta
     3541 taccggcatt tcggcccatg acaaactcac ctggctgatg gaagtgacac cggtgattat
     3601 tgtcgtgccg ctactgcttg ccaccgctaa acgttatccg ttaacgccgc tgctttatac
     3661 gctcattttc tttcacgcca tcatcctgat ggtcggcggg caatacacct acgcgaaagt
     3721 ccccgttggt tttgaggtgc aggaatggct ggggttgagc cgtaatccgt atgacaaact
     3781 gggacatttt ttccaggggc tggtgcctgc actggtggca cgagagatcc ttgtacgcgg
     3841 gatgtacgtt cgcggacgta agatggtggc gtttctggtc tgctgcgtgg cgctggcgat
     3901 aagcgcgatg tatgaattaa tcgagtggtt ggcagcgctg gcaatgggcc agggagctga
     3961 tgattttctc ggcacccagg gcgaccagtg ggacacgcaa tccgatatgt tctgcgcgct
     4021 gcttggcgca ttaacgacgg tgatattcct cgctcgcttt cattgccgcc agctacggcg
     4081 ctttggctta atcaccagag cgccggaagc gattacccct taacggtgca accagcctgg
     4141 cagccagtcg ccgtgggcgg caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc
     4201 cagcacggcg gcagtatgcg ggtccatcat cgcggcgtgg taaacacggt cgcgattttc
     4261 cgtaagaata gcttctgtca gcagcgtctg tacgttgatg ttggtttgca tcagggcggc
     4321 cagatgcgaa ggtagcgtac cggctttggt cggctgaatg ccattagcat caaccagaca
     4381 ggccacttcc acgcaacatc cttgtggcag gttatcaatc aaaccatcgt tacggacatt
     4441 gccataaatc acactcggct cgccagtcca gatagcgttc atgattgtgc tggcatattc
     4501 ccgtgacggt ttaatatcaa tccgggaggc gtttttataa tcctccagct ctttatgcca
     4561 gttcgccagc tgctcgacgc agcgtttcgg gtactcatcc agcggtactt tataacgctc
     4621 aatcaaatcc tcacgacctg gcttaataaa ccacggtgtg tactcagcaa aatgttctga
     4681 cgattctgtg acgaagtagc ccagcttttt gaacatttcg taacgcacaa tattctggca
     4741 gcgagtatta ccatgaatat ttggcttcgg tgcctgccct gcttcataag ccgccagcaa
     4801 ttcaggatag agattcacgt agctaccgtc tgcggtttta cgctccagtt ccaggtaaaa
     4861 cgccatatgg ttgatacctg cgcaacggta acgcagcgta gccggatcga tattgaggtc
     4921 acgcgccagc tcttccgccg ttccctgcac cgaatggcac agccctacct gcttgatatg
     4981 cggatagcgt gcatacatcg cccaggtatt catcgccatt gggttaacgt agttgagcat
     5041 ggtggcatcg gggcagactt ccgtcatgtc ctcgcaaatc tgccacaggt gcggaatggt
     5101 acgtagcgca cgcataatgc cgcccggccc cagcgtatcg gcaatggttt gttccagacc
     5161 atgccgctta cagacctcga aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa
     5221 tgccaccacc acaaaatcag catcctgtaa ggcttctttc tgttgggtgt ggcaggtgat
     5281 tttgccgctg gcccctgctg aatccatcaa cttgcgcacc acaatgtgtg actcttccag
     5341 gcgggtggga tcgatatcca tcagggcaat atgcgccgtt ttcagcgcct cacgatggaa
     5401 cacatcacca agaatatttt taacgaaaat cgtcgaacca gcgccgataa atgtaatttt
     5461 aggtgcagac atcatgcaga tctcctggct tgcttgaatg acttcatcat ggcaggcgaa
     5521 tctgcagaaa acctccggct tccaggttga gcattcccat aaactcatat cctaagtggc
     5581 acatcaatag atctgagttt atgggaataa attacctgaa aagctgaata atcgacccca
     5641 atcttctcat ccgctctcct cgcacctcca gggagaggaa taaaaccgtg cgcctattcg
     5701 cagtcatcct ccctcactcc tgccataatt ctgatattcc aggaaagaga gtcatccatg
     5761 aatacagata cgtttatgtg cagcagcgac gaaaagcaga cccgtagccc actgtcgctg
     5821 tattcagaat accagcggat ggaaattgag tttcgcgcac cgcatatcat gcccaccagc
     5881 cactggcatg gtcaggtcga agtgaatgtg cctttcgatg gcgatgtgga atacctgatc
     5941 aacaatgaaa aagtgaatat caatcagggt catatcacgc tgttctgggc ctgtacaccg
     6001 caccaactaa cagataccgg aacctgtcag agcatggcga ttcttaatct gccgatgcat
     6061 ctgtttctct cctggccgct ggataaagac ctgattaacc acgttactca cggcatggtg
     6121 atcaaatcac tggcgacaca gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa
     6181 ttaaacagtc cgaacgagca aattcgccag ctcgccattg atgaaattgg cctgatgctc
     6241 aagcgattta gcctctctgg ctgggaaccg attctggtca ataaaacctc gcgcacacac
     6301 aaaaacagtg tctcgcgcca tgcgcaattt tatgtcagcc agatgctggg ctttattgcc
     6361 gaaaactatg atcaggcgct gaccatcaac gatgtggctg agcacgtcaa acttaacgcc
     6421 aactatgcaa tggggatatt ccagcgggtc atgcaattga cgatgaaaca gtacataacc
     6481 gcgatgcgca tcaaccacgt tcgcgcatta ctgagcgata ccgataaaag tattctcgat
     6541 attgccctga cggcaggctt tcgttcgagt agccgttttt acagcacgtt cggcaaatat
     6601 gtcggcatgt cgccgcaaca ataccgcaaa cttagccaac agcgccgcca gacgtttccc
     6661 ggctaaagac gatatcagta tcagccaaaa aaatagttta gccgtcgata ataagcagtg
     6721 aaaataattt aaaataatca cataacatat tgtttataaa aataaatatt tcaaaaatgt
     6781 ttgtttttca cgcgctttac agcccgaaaa ggccggaaga tacttgcccg caacgaagat
     6841 tccttcataa ccgggtaagc aatgatgaaa gtattaattg ttgaaagcga gtttctccat
     6901 caagacacct gggtcggtaa cgccgttgag cgtctggcag atgctttaag ccagcaaaat
     6961 gttaccgtga ttaaatccac ctcctttgat gatggttttg ccattctctc ttcaaacgaa
     7021 gccattgact gcctgatgtt cagctatcaa atggaacatc cggacgaaca tcaaaacgtc
     7081 agacaattga tcggtaagct tcatgagcgc caacaaaacg tgccggtctt cctgttgggc
     7141 gatcgggaaa aagccctcgc cgcaatggat cgcgacctgc tggagcttgt cgatgaattc
     7201 gcctggattc tggaagatac cgccgacttt atcgccggac gcgccgttgc cgcgatgacc
     7261 cgctaccgcc agcagctatt gccgccactg ttcagcgcgc tgatgaaata tagcgacatc
     7321 catgaatatt cctgggcagc gccaggccac cagggcggcg ttggttttac caaaacaccc
     7381 gccggacgtt tctaccatga ctactatggt gaaaatctgt tccgcaccga catgggcatc
     7441 gaacgcactt ccctcggttc tttgcttgac catactggcg catttggcga aagcgaaaaa
     7501 tatgccgcac gcgtatttgg tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc
     7561 tctaaccgca ccatcatgca ggcttgcatg accgataacg atgtcgtggt cgttgaccgt
     7621 aactgccata aatccatcga acaaggcttg atgctgacag gtgcgaaacc ggtctacatg
     7681 gtgccaagcc gcaaccgcta cggcattatc gggccaatct atccgcagga aatgcaacct
     7741 gaaaccttgc agaagaaaat caatgaaagc ccgctgacta aagacaaagc cgggcaaaaa
     7801 ccgtcttact gcgtggtgac caactgtacc tatgacggcg tgtgttataa cgctaaagaa
     7861 gcgcaggatc tgctggaaaa aacctccgat cgtctgcact ttgacgaagc ctggtacggc
     7921 tatgcacgtt tcaacccgat ctatgccgat cactatgcca tgcgcggcga acctggcgat
     7981 cacaacggtc ctaccgtttt cgccacccac tccacccaca aactgctgaa tgcgctgtca
     8041 caggcctctt atattcatgt acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac
     8101 caggcctaca tgatgcatgc caccacctcc ccgctgtatg ccatctgcgc atccaacgac
     8161 gtggcggtgt cgatgatgga cggcaacagc ggcctgtcac tgacccagga agtgatcgac
     8221 gaagcggttg atttccgtca ggcgatggcg cggctatata aagagttcac cgctgacggt
     8281 agctggttct tcaaaccgtg gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc
     8341 tatgactttg ctgacgcacc aaccaaactg ctgaccaccg ttcaggactg ctgggtcatg
     8401 catccgggcg aaagctggca cggcttcaaa gatattccgg ataactggag tatgctcgac
     8461 ccgattaaag tcagcatcct tgctccggga atgggtgaag atggtgaact ggaagaaacc
     8521 ggtgttccgg cggcgctggt cacagcctgg cttggtcgcc acggcattgt gcctacccgt
     8581 accactgact tccagattat gttcctgttc tctatgggcg tgacccgtgg gaaatgggga
     8641 actctggtta acaccctttg ctccttcaaa cgccactatg acgccaacac accgctggcg
     8701 caggtgatgc cggaacttgt tgaacaatat cctgacactt acgcgaacat ggggattcac
     8761 gatctgggtg acaccatgtt tgcctggctg aaagaaaaca accctggcgc acggttgaat
     8821 gaagcctatt ccggcctacc ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt
     8881 gtcgacaaca atgtcgaact ggtatccatt gaaaatctgc caggacgtat cgcggcaaac
     8941 tcagttatcc cgtatccgcc aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat
     9001 aaaaacagtc cgcaagtaag ttatttacgc tcgctgcaat cctgggatca tcatttccct
     9061 ggatttgaac acgaaacaga agggacagaa attattgacg gtatttacca cgttatgtgt
     9121 gtgaaagcgt aaccactatt ccgctgaagg cgtaattgtt aaataacatt acgccgcctg
     9181 gcttgaggcc ttttaagtat ggcaacgttt tcataaaaat tgctgcaaac aaaaaatgtc
     9241 atgctttttg cgcggtccca ccccg
//
LOCUS       DACADA010000168         9142 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.170, whole genome shotgun sequence.
ACCESSION   DACADA010000168 DACADA010000000
VERSION     DACADA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.170"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            93..740
                     /gene="fucA"
                     /locus_tag="ISB17_004014"
     CDS             93..740
                     /gene="fucA"
                     /locus_tag="ISB17_004014"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP1981890.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            768..1916
                     /gene="fucO"
                     /locus_tag="ISB17_004015"
     CDS             768..1916
                     /gene="fucO"
                     /locus_tag="ISB17_004015"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP1981891.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2021..2866)
                     /gene="xni"
                     /locus_tag="ISB17_004016"
     CDS             complement(2021..2866)
                     /gene="xni"
                     /locus_tag="ISB17_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAP1981892.1"
                     /translation="MRGLFPISHPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHNEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(2888..4255)
                     /gene="sdaB"
                     /locus_tag="ISB17_004017"
     CDS             complement(2888..4255)
                     /gene="sdaB"
                     /locus_tag="ISB17_004017"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAP1981893.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4313..5602)
                     /locus_tag="ISB17_004018"
     CDS             complement(4313..5602)
                     /locus_tag="ISB17_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1981894.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(6159..7523)
                     /gene="ppnN"
                     /locus_tag="ISB17_004019"
     CDS             complement(6159..7523)
                     /gene="ppnN"
                     /locus_tag="ISB17_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAP1981895.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(7635..8483)
                     /gene="queF"
                     /locus_tag="ISB17_004020"
     CDS             complement(7635..8483)
                     /gene="queF"
                     /locus_tag="ISB17_004020"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAP1981896.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQLAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8551..9096
                     /gene="syd"
                     /locus_tag="ISB17_004021"
     CDS             8551..9096
                     /gene="syd"
                     /locus_tag="ISB17_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAP1981897.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
BASE COUNT         2347 a         2243 c         2380 g         2172 t
ORIGIN      
        1 caggaagaat atggtgagta attaaacggc taattcaata gtgtgaaagg aacaacatta
       61 ttgccctgtt ttgaatcaga gagaggtagc taatggaacg aaataaactt gctcgtcaga
      121 ttattgacac ttgcctggaa atgactcgcc tggggctgaa ccagggaaca gcgggtaacg
      181 tcagtgtacg ttatcaggat gggatgctga ttacgcctac aggtattcca tatgaaaaac
      241 tgacggagtc gcatattgtc tttattgatg gcaacggcaa acatgaggaa ggaaaactcc
      301 cctcaagcga gtggcgtttc catatggcgg cctatcaaag cagaccggat gccaacgcgg
      361 ttgttcacaa tcatgccgtt cattgtacgg cagtttccat tcttaaccga ccgatccccg
      421 cgattcacta catgattgcg gcggctggcg gtaattctat tccttgcgcg ccttatgcga
      481 cctttggaac acgcgaactt tctgaacatg ttgcgctggc tctcaaaaat cgtaaggcaa
      541 ctttgttaca acatcatggg cttatcgctt gtgaggcgaa tctggagaaa gcgttatggc
      601 tggcgcatga agttgaagtg ctggcacaac tttacctgac gaccctggcg attacggacc
      661 cggtgccagt gctgagcgat gaagagattg ccgtagtgct ggagaaattc aaaacctacg
      721 ggttacggat tgaagagtaa tttcgtaaag caacaaggag aaggatgatg gctaacagaa
      781 tgattctgaa cgaaacggca tggtttggtc gaggtgctgt tggagcttta accgatgaag
      841 tgaaacgccg tggttatcag aaggcgctga tcgtcaccga taaaacgctg gtgcaatgcg
      901 gcgtggtggc gaaagtgacc gacaagatgg atgccgcagg actggcatgg gcgatttacg
      961 acggcgtagt acctaaccca acaattactg tcgtcaaaga agggctcgat gtattccaga
     1021 atagcggcgc ggattacctg atcgctattg gtggtggttc tccacaggat acgtgtaaag
     1081 cgattggcat tatcagcaac aacccggagt ttgccgatgt gcgtagcctg gaagggcttt
     1141 ccccgaccaa taaacccagt gtaccgattc tggcaatccc caccacagca ggcactgcgg
     1201 cagaagtgac cattaactac gtgatcactg acgaagagaa acggcgcaag tttgtttgcg
     1261 ttgatccgca tgatatcccg caggtggcgt ttattgacgc tgacatgatg gatggtatgc
     1321 ctccagcgct gaaagccgcg acgggtgtcg atgcgctcac tcatgctatt gaggggtata
     1381 ttacccgtgg cgcgtgggcg ctaactgatg cgctgcacat taaagcgatt gaaatcattg
     1441 ctggggcgct gcgaggatcg gttgctggtg ataaggatgc cggagaagaa atggcgctcg
     1501 ggcagtatgt tgcgggtatg ggattctcga atgttgggtt agggttggtg catggtatgg
     1561 cgcatccact gggcgcgttt tacaacacgc cacacggtgt tgcgaacgcc atcctgttac
     1621 cgcatgtcat gcgctataac gctgacttta ccgatgagaa gtaccgcgat atcgcgcgcg
     1681 ttatgggcgt gaaagtggaa ggtatgagcc tggaagaggc gcgtaatgcc gccgttgaag
     1741 cggtgtttgc tctcaaccgt gatgtcggta ttccgccaca tttacgtgat gttggtgtac
     1801 gcaaggaaga cattccggca ctggcgcagg cggcactgga tgatgtttgt accggtggca
     1861 acccgcgtga agcaacgctt gaggatattg tagagcttta ccataccgcc tggtaaatgt
     1921 gctgatgtga taatgccgga tgcgacgcta gccgcgtctt atccggcata taagtgcatt
     1981 aaattcaatg tgttgataat cgtttgcatc cggcacgccg ttaccttacc aaccgcaatt
     2041 gctgtaaatt gccgtcgata tgcaaatcgg tttgtaagcg ggcaatatcg cggcacagaa
     2101 acgccatctc tttatgggtt tctaattttt tgcgccactt ttcggcaacc gcatccagat
     2161 tctcatatat cccttccaga ctctgaaact cgaccagtag ctgcgtggcg ctttttggtc
     2221 cgatccccgc aacacccggt acctttgaac tgctgatccc cgcaagcccc cagtaatcgg
     2281 gcaactgctg cggttgaacg ccaaattctt tatcgataaa tggcgcatcc agccaacgtt
     2341 tctggaagta atcacgaata cgtaatgtcg gtgaaagtaa ctgacagtag cctttatctg
     2401 tcgaaacaat cgttgcctga tgcccggcct gtgtcacttt gaccgccagc gtggcggcta
     2461 agtcatcggc ttcgttgccg ctggcggacc agcacgggac gccgcgttgc tcaaaggcgg
     2521 cgcgtaatgc aggcatctcg ttgtgcaact cttccggcat tggcggtcga cccgctttgt
     2581 aatctggtaa acgctgatga cgccagccgc tactgcggtt ttcatcatca aaaacggcga
     2641 ccgcgtgggt tggctggctg tgcataatga gctgatcgag cgcgtgctgg caggtttcga
     2701 cacagggcga accctgaacg gcatgaatgc gacgaataag attcagtgca tcgacaataa
     2761 gcaaatgaac agccacgata acccccttaa agatcaggcg ataagggtaa cattcgatgc
     2821 cagaacaggc tatggctgga tgagaaatcg ggaagaggcc tcgcaaaaag aggcctctgg
     2881 agagcgatta atcgcaggca acgatcttca ttgccaggcc gccgcgagag gtttcgcggt
     2941 acttggcgtt catatcttta cctgtttcgt acatggtctc gataacttta tcgaggcaga
     3001 cgcgcggctc gctggtacgg cgcagcgcca tacgcgcggc gttcaccgct tttactgccg
     3061 caatggcgtt acgctcgatg cacggtacct gcacctgtcc agcaaccggg tcacacgtca
     3121 gaccgaggtt atgctccatg gcgatttccg ccgcgatgca cacctgcgcc gggcttgcgc
     3181 ctaacagttc tgccagacca gctgccgcca tagagcacgc cacgccgact tcaccctggc
     3241 agccgacttc ggcaccagaa atcgaggcgt tcatcttata aagagaacca atggcgctgg
     3301 ctaccagcag gtaacgcgcc agtgagttag cgttcacttc gcggataaac ttgtcgtagt
     3361 acgccagcac ggccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac
     3421 cgccagcagc gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaactaccg
     3481 ccatcgggtc agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac
     3541 ggcgtggaac gcgcagtttg ccaggtaaca cgccttcggt agaaataccg cgttcaatac
     3601 cgccacgcat gacttcccag acgttcgcca ggtgctgttc cagctcttct ttgctgtgca
     3661 gcgccagctc gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt
     3721 tttgtagatc ggcagcggaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct
     3781 gaccaaaatg ctcttcatca acgataaagc caccgccaat agagtaataa gtctggctgt
     3841 aaacgacttt atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt
     3901 tgtcggcatg gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg
     3961 ccagcatcag gcgaccatga gtattcacat cctgaataaa accagggatg gaatcgatat
     4021 ccacggtatc cggcaggtta cccgccagac ccataattat ggcgatatct gtgtggtggc
     4081 ctttaccggt cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa
     4141 gcaggttacg ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg
     4201 tatgagaact ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa
     4261 atacatcgcg ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga
     4321 acagagagta gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc
     4381 tgatgtgacc gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca
     4441 ggaacaggat catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg
     4501 ggttcagggt ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca
     4561 gcttgttgat ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga
     4621 agccttcacg tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga
     4681 taatcggagc catccacgcg atgatcggcg cgttaaagtg gttagccagg taagacagaa
     4741 tcgagatgtt ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc
     4801 tgaagacgaa gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttag
     4861 agcatttctg ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga
     4921 tgatcggaga gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc
     4981 acagaccgtt tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt
     5041 tccactgcgg gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca
     5101 gaatactcat cgctttaacg atcatctgct caccgaagcg aacgatggtc atcataccca
     5161 cgatcaggat cagcgacaga atcgcacgtg gcggtggcgt catacccagt tggtgagaca
     5221 tgaagctttc aactgtattg gtgattgcca cgctataaac cagcaggatc gggtagatag
     5281 cgaagaagta gagcagagta atcagtttac ctgcgccaat accaaagtgt tcttctacaa
     5341 cctcggtgat gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt
     5401 gagcaaaaaa cgtcatcggg aatgcaagga tagccatgat gatcagcggg atcataccgc
     5461 caacaccggc gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc
     5521 ccagcatcca catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg
     5581 tgctggtttg agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta
     5641 gccagttcag cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc
     5701 ttaatcattt tccaccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta
     5761 tcagtgatct tgatcccaaa tacaataatc tacttggaag atagataatc acaacgcttt
     5821 actctgtaaa tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc
     5881 tgacttcttc tggggcgcgc aatatatgta ggttgaactg gaaaaatcaa tacttttagt
     5941 taatcatggc gataatttct ttttcatggc gataaaatgt ggtttatgaa aatagggaaa
     6001 cgtttgcgtt tcaacataaa ttgtatgaca tattaactgt ggtgatattt gtcgcgtgag
     6061 gttggtaaga aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg
     6121 ataaaacccg gcaacaaacg tcaccggggg aggggagatt acgtgcagat ttcgtagcaa
     6181 gggatgtagg ctgagcctgg caacttcata cgatgctggg caacaaaacc ctgtagcagg
     6241 tcgtccatac gacgcataat ctctttgtcg ccgttgattt tgtaaggacc aaactcttca
     6301 atggcgcgaa taccgacttc ttttacatta cccgccacaa taccggagaa cgcacggcgc
     6361 aggtcggcag ccagcacttc aacgggttga tccgggtaaa gcttcagatt agccatattc
     6421 tcgtgagacg gctcaaacgg catttgcaaa tctggcgcaa tgcgcattga ccagttaaag
     6481 ctgtaagcgt caccagtgtc gcggcgattt tctttcacca gcggcatcga ttttttcatc
     6541 tgacgagcga cttcagcggc gtcatcaatg atgatgcggt aatggcggcg cgcgttttca
     6601 cccagcgtat gcacgacaaa ctcgtccagt acgcggaagt agtcggcgct ctctttcggg
     6661 ccggtgagga tcaatggtaa aacctgatct ttgttggccg ggttcattaa aattcccagc
     6721 aaatacagca actcttctgc tgtacccaca ccgccaggga agataatgat accgtgagcg
     6781 atacggacaa acgcttccag acgtttttcg atatctggca tgatgatcaa ttcgttgacc
     6841 agcgggttag gcggttcagc ggcgataatc gacggctctg tcataccaat aaagcgactg
     6901 tctttgtaac gctgctgcgc gtgtccgacc gcagcacctt tcatcggcgc ttccatcgct
     6961 cccggaccac agccggtgca gatattcagt tcacgcaggc ccaactgatt cccgacgcga
     7021 cgggcataca aatactcgtt ttcgttaatt gagtgaccgc cccagcagac caccatattt
     7081 ggcgcttcac cgacatgcag cgcgcgagcg ttacgcaaga tggaaaagac caggttagtg
     7141 atgtggacgg agttgtcgag attcaggttg ggaaaacgaa cggtgttatg aatttgccca
     7201 taaacgaaga gaatgtcacg cagaaccgcg aacaagttgg cctgcaaagc gcgaataatt
     7261 cgcccatcga caaaagcctc ttccggggga tttatcagtt ccagctttac gccgcgttca
     7321 cgtcgcagga cgttaatatc gaaattttca aaacgagaca gcaattcttt gctgttatcg
     7381 gtcaaactac cggagttcag tacggcaagt gaacagttgc gaaacagttg atagaggtcg
     7441 ctgctggcgg tgcgtttaag catatccact tccagctgcg acaacatatc catggagcca
     7501 agcgggctaa tatgtgtaat caagaaaaac tccttatggg acgaatatcg tcattccctt
     7561 tgattacaat agccctgcct gatgaccttt cacaaccttc tgcgtgaatc cagcacgcaa
     7621 aattgagaaa aaatttattg ccgaaccagt cttgtggtcg atgggacaaa atcgctatta
     7681 ctgcgccacg ggttaatgtc cagaccgcca cgacgggtat aacgtgcgta aacgctcaat
     7741 ttctctggct ggcagaagcg taacaggtca ttaaagatgc gttccacgca ctgttcgtgg
     7801 aactcgttgt gatgacggaa agagaccagg taacgcagca gtttttctct gtcaatttgg
     7861 cgtccacgat actgaatttg gatggaaccc caatctggtt gatgggtgat caggcagttt
     7921 gatttcagca ggtggctgac aagcgtctct tccactactt tttcaccgct ggtggcattc
     7981 tccagatagt cagtagtgaa ttcatagtta tcgatagtga tatcctggtc atcaatgcag
     8041 gtgccattga aatgacctat cggctggcct tccagttcat caagacgata taacgccacg
     8101 ctaaccttac cctgggcgca agtgcttaag tcgcgctcta gcgtctggcg cacctcatcc
     8161 cagttattaa aacgcgtctg gttaaaactg ttgagataga gcttaaaact cttcgactca
     8221 atcagattta cgctggtgta atcaagttca acatgaccga cagcgagttg cggcaaacct
     8281 ttcgcattca gccaggaaag ttcatacagc gtccagatat ccgtaccgtg aaaaggcagg
     8341 ttatccgctt tcagacccag cgggtcgcga ttcaggctgc gtggaacgcc ttgcagtagg
     8401 ctggcgtcat aggtatcccg gtaatcggtt gattttccaa gtgtcaggcc cgcaagtgcc
     8461 tgatggtttg cataagaaga catgtttcac cgtcataaac cagataaact aaacgacaag
     8521 tgtatctggt ttaagtgaag agagaaatta gtggacgatt tgaccgcacg agccctgaaa
     8581 gattttactg cacgctactg cgatgcgtgg catgaagagc ataaaagctg gccgttaagt
     8641 gaggaactgt acggcgttcc ttcaccgtgc attatttcaa ctaccgaaga tgccgtatac
     8701 tggcaacccc agccgtttac cggggaacaa aatgtaaacg cggttgaacg cgcttttgat
     8761 attgtgatac aacccacgat tcataccttt tatacgactc agtttgccgg ggatatgcac
     8821 gcgcagtttg gcgatatcaa actgacattg ctgcaaacct ggagtgaaga cgacttccgt
     8881 cgggtgcagg aaaatctgat tggtcatctg gtgactcaga aaaggctaaa actgccgccc
     8941 acgctattta tcgcgacgct ggaagaagag ttagaggtca tctcggtgtg taatctctcc
     9001 ggtgaagtgt gcaaagagac gctgggaaca cgtaaacgga cgcatttagc ctcaaatctt
     9061 gcggaattcc ttaatcaact taagcctctt ctgtaatcca aataccccat ctgattgtga
     9121 gagatctctt acagactctg ta
//
LOCUS       DACADA010000169         9139 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.171, whole genome shotgun sequence.
ACCESSION   DACADA010000169 DACADA010000000
VERSION     DACADA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.171"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(57..1433)
                     /gene="trkA"
                     /locus_tag="ISB17_004022"
     CDS             complement(57..1433)
                     /gene="trkA"
                     /locus_tag="ISB17_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAP1981898.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1455..2744)
                     /gene="rsmB"
                     /locus_tag="ISB17_004023"
     CDS             complement(1455..2744)
                     /gene="rsmB"
                     /locus_tag="ISB17_004023"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP1981899.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2796..3743)
                     /gene="fmt"
                     /locus_tag="ISB17_004024"
     CDS             complement(2796..3743)
                     /gene="fmt"
                     /locus_tag="ISB17_004024"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP1981900.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(3758..4267)
                     /gene="def"
                     /locus_tag="ISB17_004025"
     CDS             complement(3758..4267)
                     /gene="def"
                     /locus_tag="ISB17_004025"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP1981901.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4397..5521
                     /gene="dprA"
                     /locus_tag="ISB17_004026"
     CDS             4397..5521
                     /gene="dprA"
                     /locus_tag="ISB17_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP1981902.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5493..5966
                     /locus_tag="ISB17_004027"
     CDS             5493..5966
                     /locus_tag="ISB17_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAP1981903.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5995..6537
                     /locus_tag="ISB17_004028"
     CDS             5995..6537
                     /locus_tag="ISB17_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981904.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            6542..7114
                     /gene="tsaC"
                     /locus_tag="ISB17_004029"
     CDS             6542..7114
                     /gene="tsaC"
                     /locus_tag="ISB17_004029"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAP1981905.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7119..7937
                     /gene="aroE"
                     /locus_tag="ISB17_004030"
     CDS             7119..7937
                     /gene="aroE"
                     /locus_tag="ISB17_004030"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP1981906.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            7934..8191
                     /locus_tag="ISB17_004031"
     CDS             7934..8191
                     /locus_tag="ISB17_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAP1981907.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8167..8721)
                     /locus_tag="ISB17_004032"
     CDS             complement(8167..8721)
                     /locus_tag="ISB17_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP1981908.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         2312 a         2212 c         2257 g         2358 t
ORIGIN      
        1 aattaatcct taataatcaa agggaaataa taaggcgtca ttagacgcct tattaattac
       61 aagaagaaag gacttggctg gaagagtctt tcgacgtcgg taataaactt tttatctgtg
      121 aggaacataa ttacgtgatc gccttgctca atgcgcagat tgtcattggc aatcatcaca
      181 tcgtttccac gtaccaccgc tccaataatc gttcctggcg gtagcttgat ttcgtcaatg
      241 actctgccga caacgcgtga ggtgctttca tcaccgtgag caacggcttc aatagcttct
      301 gctacgccgc ggcgtaatga ggaaacacca acaatatctg cttttcgcac atggctaagc
      361 aacgcagaaa tagttgcttg ttgtggtgaa atcgcaatat cgatcacgct cccctgaacc
      421 agatccacat aagcgcgacg ttggatcaat accatcacct ttttcgcacc catacgtttg
      481 gcaagcatgg cggacatgat attggcctcg tcatcgttgg tgacggcaat aaacagatca
      541 acttgatcga tatgttcttc ggccagcagt tcttgatccg acgcatcacc aaaaaagacg
      601 atcgtattct gcaacttttc cgccagttcg gcagcgcgct gctgattacg ttcgatgagt
      661 ttaacgctgt aatctttttc cagacgacgc gccagccctg caccgatatt accgccacca
      721 accagcatga tccgcttata cggtttttcc agtcgctgta attcactcat caccgcacgg
      781 atatgctgtg aagcggcaat aaagaacact tcatcaccag cttcaacaat ggtcgaacct
      841 tgcggacgaa tggggcgatc gtggcggaaa attgctgcca cacgagtatc gatatgtggc
      901 atatgttcgc gcatggtcga aagtgcatta ccaatcagcg ggccgccata ataggcttta
      961 accacagcca ggctgacttt accctcagcg aagttcacca cctgcaatgc gccgggatac
     1021 tcaatcagtc ggtaaatatt atcgataacc aactgctctg gtgcgatcag atgatcaatc
     1081 ggtacagcat ctgaatgaaa tagtttatcg gcatcgcgca cgtagtctgg tgagcggata
     1141 cgagcgatgc gattaggggt gttgaaaagt gagtaggcta cctggcaggc aaccatattg
     1201 gtttcatctg aactggttac agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc
     1261 aatacgcgtg gatgagagcc atgcccctgc acgacccgca ggtcaaattt atcctgtaag
     1321 gtccgcagac gctcaccgtt ggtatcgaca acagtaatat cgttgttctc gccaaccagg
     1381 ttttccgcca gtgtgccgcc aacctggccg gcacccagaa tgataatttt catcagtcgc
     1441 gacccgttat ctcatcactt tttgattagc ttagcgtaaa agaagccgtc gccctcttcg
     1501 gcaccgggca aattttgttt acccggttgc tctggtgttc ctgtttcaca aagttcggca
     1561 tcagcggtac gttgcaaaaa ggctttaatc tgcaggctat tttcttccgg taacaccgaa
     1621 caggtggcat agaccagggt tccaccggat tttaaatgtg gccaaatggc atcgagtatt
     1681 tcagactgca attgcgcgag ttccgggata tcgcgatcac gacgtaacca tttaatatcc
     1741 ggatggcgac gaatcacccc cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac
     1801 tgttgctcgc cacaccattg ggaagggttg cggccatcac cttgtttcac ggtcgccttc
     1861 ataccaaggc gttttaaatt gtcgtaaacg cgagagaggc gctgctcatc aatatcgacc
     1921 gccagaactt gcgcctctgg agcaacctca agaatatgcg ttgttttacc gcctggggcg
     1981 gcgcaaagat ccagaatgtg ttcaccattt tgtggagcaa gccaggtcat gcaaccttgt
     2041 gctgaagcat cctgaacggt aacccatcct tcttcaaaac cgggtagcgc atgaacaggt
     2101 gcaggtgttt ccagctgtac agcatcaggg taatccgcat gcgggaaacc tttcattcct
     2161 gcttcatcca gcaatgcaag ccagctgtcg cgggaatgat gcgtacgatt aacgcgcagc
     2221 cacattggcg gacgctggtt attggcttcg acgatggatt gccactgctc gggatacgcc
     2281 ttttgcagac gcttcagcaa ccaggaagga tgcagataac gtgcatcact ggcattaaac
     2341 tcggctaata actcatcttg ctgacgctgg aactgacgta atacaccgtt aatcaatccc
     2401 ttaagttgcg ggcgcttaat tgcaactgcg ccttcaaccg tttcagccag cgcggcatga
     2461 ggtggaatgc gggtataaag cagttgatac aaaccaacca taatcaggta atgaacagta
     2521 cgctgtttgc ctgtcatcgg acgggccatt agcttattaa tcagccagtc taactgtgag
     2581 agcgtacgca gtacgccaaa gcataactcc tggagaagag ctttatcttt atcggaaact
     2641 ttttgctgga gcggtggcag aatgttgctc agtgattgcc cttgctcaac gacttgttca
     2701 atggcctggg ccgccatgct acgtaaatta cgttgttttt tcataagtat aaatataaag
     2761 atgcccggta agaccgggct tagaagagtg gactatcagg ccagacggtt gcccggtaca
     2821 aaccattccc gacgagaatt caggaggtct tgcacgctca tcgctttctt acctgcaggt
     2881 tgcattgaga gcagattcag gatgccatca cctgtcgcta cctgaatgcc ttgtttattg
     2941 gcttcaagga ttgttcctgg cgcagccttt gttgtcgtat cgatgacaga tgctttccag
     3001 actttaaccg gctgcccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
     3061 atgcaacgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
     3121 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
     3181 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
     3241 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
     3301 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
     3361 agtgagcgtt ggattggtgc tgcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
     3421 atacagccaa gacgcggcat ctccagcaca gctttaggca gaattaaacc ataggcgacg
     3481 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
     3541 gaaacaggtt gaaaaacggg cagacctttg tcctcagcca gaactttaac cgggctgggc
     3601 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgatgtta
     3661 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
     3721 ataatacgta gtgattctga cacgttaatt cttatcctta agcccgggct ttcagacgat
     3781 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
     3841 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
     3901 cttccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
     3961 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
     4021 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
     4081 catcaattac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
     4141 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
     4201 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
     4261 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
     4321 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
     4381 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag
     4441 cttgtatggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
     4501 tgcggttgta ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
     4561 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
     4621 ccctgcggac agcgaatttt atcctcctca acttctggcg acgacagatt accccggcgc
     4681 actgtttgtt gaaggagaac tgcacgcgtt gcattcattt cagcttgccg tagtggggag
     4741 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
     4801 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
     4861 ggccttacag gtaaatggcg ttagcattgc tgtattgggg aatggactta ataccattca
     4921 tccccgccgc catgctccac tggctgccag tctacttgag caaggtggtg ctctcgtctc
     4981 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
     5041 cagtggtcta agtaagggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
     5101 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gctttgccag gtccaatagg
     5161 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
     5221 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
     5281 aaattcattt tattcaccag atcaggaaga cgtggcattg ccatttcctg agctcctggc
     5341 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
     5401 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
     5461 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
     5521 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
     5581 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
     5641 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
     5701 tgcgtattta tacccctgaa gagtgtgaaa gactggatgc cagctgtcgt ggttttttgc
     5761 ttttccttga gcaaattcag gtgcttagcc ttgaaacccg tgaaatggtg atagagcgag
     5821 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
     5881 tgttgttcaa tattccgggt tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
     5941 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
     6001 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaacta
     6061 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcatgtggc
     6121 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
     6181 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
     6241 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
     6301 gcaattacat gcccccaatg ccggacgggc catctggttc agcgccgctc ccgttatggc
     6361 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
     6421 atagctggag aatgccctga gtgccattat ccgctactca tcgaaaagaa aaccgcgcag
     6481 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cgatttcggc ggaataataa
     6541 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
     6601 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
     6661 aacagcagtg atgagactgt tggagttaaa acggcgtccg gttgataagg ggctgatttt
     6721 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgacgc
     6781 gcagcgtgaa accatttttt cccgctggcc aggtcctgta acctttgtct ttcccgcgcc
     6841 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
     6901 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
     6961 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
     7021 tgcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
     7081 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
     7141 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
     7201 atattgaaca tccctatggg cgtgtgttga cacccatcaa tgatttcatc aacacactga
     7261 acactttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
     7321 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
     7381 tcaagcggtt agaagatgga cgcttgctgg gtgacaatac cgatggtata ggtttgttaa
     7441 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattttgctc atcggcgctg
     7501 gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg gtgacaatca
     7561 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
     7621 ttcacgcgtt gggtatgcac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
     7681 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
     7741 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
     7801 agcgaggctc aaagcgtact gctgatggtt taggaatgct ggtggcacaa gcggctcatg
     7861 ccttccttct ttggcatggc gttctgcctg atgtagaacc ggtcatcaaa ctattgcaac
     7921 aggagttgtc agcgtgaatc aggccatcca gtttccggat agggaagagt gggacgagaa
     7981 taaaaaatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat gtgcgatctc
     8041 tggcgagagt ctggcgtatc gctttactgg agatacacca gaacagtggt tagagagttt
     8101 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
     8161 agatgatcaa ggttgggtgt ggttaccctg atccatatat tcgtccttcc atttcacgta
     8221 gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
     8281 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccaggct
     8341 acctgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
     8401 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaatagt
     8461 gacatcttcg ccaatggtta atgggttgcc agctgggttg tacgaggatt tatgagtgac
     8521 gtgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
     8581 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
     8641 cacgacgctg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
     8701 tgggcgtaaa acctcagaca tactcacctc aacaataaat gatttactaa tgactttggg
     8761 tacattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
     8821 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
     8881 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
     8941 atagtcgtaa caacaaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag
     9001 cgtaatatac gccacctcgc gacagtgagc tgaaaaccgc gtcgcaactg ctctttaaca
     9061 atttatcaga caatctgtgt gggcactcga agatacggat tcttgacgtc gcaagacgaa
     9121 aaatgaatac caagtctca
//
LOCUS       DACADA010000170         9008 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.172, whole genome shotgun sequence.
ACCESSION   DACADA010000170 DACADA010000000
VERSION     DACADA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.172"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            16..1158
                     /locus_tag="ISB17_004033"
     CDS             16..1158
                     /locus_tag="ISB17_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1981909.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1165..1929)
                     /gene="glcC"
                     /locus_tag="ISB17_004034"
     CDS             complement(1165..1929)
                     /gene="glcC"
                     /locus_tag="ISB17_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAP1981910.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2180..3679
                     /gene="glcD"
                     /locus_tag="ISB17_004035"
     CDS             2180..3679
                     /gene="glcD"
                     /locus_tag="ISB17_004035"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAP1981911.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3679..4731
                     /gene="glcE"
                     /locus_tag="ISB17_004036"
     CDS             3679..4731
                     /gene="glcE"
                     /locus_tag="ISB17_004036"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAP1981912.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4742..5965
                     /gene="glcF"
                     /locus_tag="ISB17_004037"
     CDS             4742..5965
                     /gene="glcF"
                     /locus_tag="ISB17_004037"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAP1981913.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5970..6374
                     /locus_tag="ISB17_004038"
     CDS             5970..6374
                     /locus_tag="ISB17_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAP1981914.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6396..8567
                     /gene="glcB"
                     /locus_tag="ISB17_004039"
     CDS             6396..8567
                     /gene="glcB"
                     /locus_tag="ISB17_004039"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAP1981915.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVTFKVVDKQLRIQLKNGKETTLRTLAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8922..>9008
                     /locus_tag="ISB17_004040"
     CDS             8922..>9008
                     /locus_tag="ISB17_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease"
                     /protein_id="HAP1981916.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFF"
BASE COUNT         2151 a         2273 c         2576 g         2008 t
ORIGIN      
        1 gttatccaca gagatatgga atgcgatctg ctgatgatta aaattgaaaa agtaattaat
       61 aaaaatgacc ttaaggcatt tatcgctttt ccgtcatcac tttatcccga cgatccaaac
      121 tggatacccc ctttattcat tgaacgcaac gagcatttgt ctgcgaaaaa tccggggacg
      181 gaccatatca tctggcaggc gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt
      241 accgcgcaaa tagatacctt gcaccgcgag cgttacggca aagataccgg tcatttcggc
      301 atgattgacg ccattgatga tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg
      361 tggttgaagt cacaaggtgc aagtaagatc agcggtcctt tcagcctgaa tatcaatcag
      421 gaaagcggat tactgattga aggtttcgat acaccaccct gtgcgatgat gccgcacggt
      481 aaaccgtggt atgccgcgca tattgaacaa ttgggttatc acaaaggcat tgatttactg
      541 gcgtggtgga tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa actgatggat
      601 caggtgcgta agaaggtgac cattcgctgc atcaatcgcc agcggtttgc cgaagagatg
      661 cagatcctgc gtgagatttt caattctggc tggcagcaca actggggatt tgtgccgttt
      721 accgaacatg aattcgcgac catgggcgac caacttaaat atctggtgcc ggacgatatg
      781 atctacatcg ctgagattga ttctgcaccc tgcgcgttta ttgtcggctt gccaaacatc
      841 aacgaggcga ttgccgatct gaacggatcg ctctttccct tcggctgggc aaaattgctg
      901 tggcgtttaa aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg cgtgcgggac
      961 gagtatcagt tcagccgcat cggcccggtg attgcgttgt tattgattga agccttacgc
     1021 gatccgtttg cccgccggaa gatcgatgcg ctggagatgt catggatcct cgaaaccaat
     1081 acaggcatga ataacatgct ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg
     1141 tatgaaaagc agatttgatg gcagtcaccc ctgcttcatc tccagcggca ccgccgagcg
     1201 aatcaaatgg tggccttcca gctcgatatc gtggagattc tttttcacgg tccgcacatg
     1261 atcgcgtgct gcgcgctggg cgacgtgcgg cagccgctgc aacaccgcgt tgtagatccg
     1321 cgcatgctgg cgatcgatct gctttttttg ctgtggtcga tggtagagat tatttacgct
     1381 ggcaaacacc gaattaaaca tcaggtcggt caatgattgc agcgtaaact ccagcacctg
     1441 attgtgagaa gcctgacaaa tggcgagatg gaaagcgtga tccaactgcg catgttcgat
     1501 cagcgaaatc tctttgttgt tctcactggc ggcgagcatt ttttcataac agcgggttat
     1561 cacaacaaaa tcagcctggg ttcccagcgt tgccgccagc cttgccgatt cgccctccag
     1621 taatgcgcga acgtcgagca gatcgtacag cgttcgcggc tgcgtactga acagatggat
     1681 cagcgggctg gtgtcctgca cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc
     1741 cgtttcaata atcccgcgcc cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga
     1801 gaagccgagc ttttcacaca gtcgacgttc cgagggaagc ggctgaccga ccttcagtac
     1861 gccgtcgata attaaccgtt cgatactctc tgcaaccact tcgcaaatag ggcgacgttc
     1921 atctttcatt cccggacctc gtgcacaggt aggaccaatt tttctgttct taactcgcaa
     1981 aacacgcaca tcacgtaagt gattatgtta catcaattta acattaagtt aaccaagaca
     2041 aggtcacaga gctggaaaaa aaatggtctg accggtagct aaagagatag acgaaaacga
     2101 aaagcccgct taataactgt tcacagaagc agcgcgaaaa aatcagctgc cacacaacac
     2161 aacaaagcga agcctactca tgagcatctt gtacgaagag cgtcttgatg gcgctttacc
     2221 cgatgtcgac cgcacatcgg tactgatggc actgcgtgag catgtccctg gacttgagat
     2281 cctgcatacc gatgaggaga tcattcctta cgagtgtgac gggttgagcg cgtatcgcac
     2341 gcgtccatta ctggttgttc tgcctaagca gatggaacag gtgacggcga ttctggctgt
     2401 ctgccaccgc ctgcgtgtac cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg
     2461 cgcgctgccg ctggaaaaag gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga
     2521 cattaacccc gttggtcgcc gtgcgcgcgt gcagccgggc gtgcgtaacc tggcgatctc
     2581 ccaggccgtt gcgccgcata atctctatta cgcgccggat ccgtcctcac aaatcgcctg
     2641 ttcgattggc ggcaatgtgg cggaaaatgc cggtggcgtt cactgcctga aatatggtct
     2701 aaccgtacat aacctgctga aaattgaagt gcaaacgctg gatggcgagg cgctgacgct
     2761 gggatcggac gcgctggatt cgcctggttt tgacctgctg gcgctgttta ccggatcgga
     2821 aggtatgctc ggcgtgacca ccgaagtgac gttaaaactg ctgccgaagc cgcccgtggc
     2881 gcgggtgctg ttagccagct ttgactcggt agaaaaagcc ggacttgcgg ttggtgacat
     2941 catcgccaat ggcattatcc ccggcgggct ggagatgatg gataacctgt cgatccgcgc
     3001 tgcggaagat tttatacatg ccggttatcc cgtcgatgcc gaagcgattt tattgtgcga
     3061 gctggacggc gtggagtctg acgtacagga agactgcgag cgggttaacg acatcttgtt
     3121 gaaagcgggc gcgactgacg tccgtctggc acaggacgaa gcagagcgcg tacgtttctg
     3181 ggccggccgc aagaacgcat tcccggcagt ggggcgcatt tccccggatt actactgtat
     3241 ggatggcacc atcccacgcc gcgcgctgcc tggcgtactg gaaggcattg cccgtttatc
     3301 gcagcaatat gatttacgcg ttgccaacgt ctttcatgcc ggagacggca acatgcaccc
     3361 gttaatcctt ttcgatgcca acgaacccgg tgaatttgcc cgcgcggaag agctgggcgg
     3421 gaagatcctc gaactctgcg ttgaagttgg cggcagcatc agtggggaac atggcattgg
     3481 gcgcgaaaaa atcaatcaaa tgtgcgccca gttcaacagc gatgaaatca cgaccttcca
     3541 tgcggtcaag gcggcgtttg accccgatgg tttgctgaac cctgggaaaa acattcccac
     3601 gctacaccgc tgtgctgaat ttggtgccat gcatgtgcat cacggtcatt tacctttccc
     3661 tgaactggag cgtttctgat gctacgcgag tgtgattaca gccaggcgct gctggagcag
     3721 gtgaatcagg cgattagcga taaaacgccg ctggtgattc agggcagcaa tagcaaagcc
     3781 tttttaggtc gtcctgtcac cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt
     3841 aattacgacc cgaccgagct ggtgataacc gctcgtgccg gaacgccgct ggtggcgatt
     3901 gaagcggcgc tggagagcgc cgggcaaatg ctcccctgcg agccgccaca ttatggtgaa
     3961 gaagctacct ggggcgggat gatcgcctgc gggctggcgg ggccgcgtcg cccgtggagt
     4021 ggttcggtcc gcgattttgt cctcggcacg cgcatcatta ccggcactgg aaaacatctg
     4081 cgttttggtg gtgaagtgat gaaaaacgtt gccggatacg atctctcacg gttaatggcc
     4141 ggaagctacg gttgtctggg tgtgctcact gaaatctcga tgaaagtgtt accgcgacca
     4201 cgcgccaccc tgagtctgcg tcgggaaatc agcctgcaag aagccatgaa tgaaatcgcc
     4261 cagtggcaac tccagccatt acccattagt ggcttatgtt acttcgacaa tgcgttgtgg
     4321 atccgccttg agggcggcga aggatcggta aaagcagcgc gtgaactgct ggggggcgaa
     4381 gaggttgcgg gtcagttctg gcagcaattg cgtgaacaac aactgccgtt cttctcgtta
     4441 ccaggtacct tatggcgcat ttccttaccc agcgatgcgc cgatgatgga tttacccggc
     4501 gagcaactga tcgactgggg cggggcgtta cgctggctga aatcgacagc cgatgacaat
     4561 caaatccatc gcattgcccg caacgctggc ggtcatgcga cccgctttag tgccggagat
     4621 ggtggttttg ccccgctacc ggctccttta ttccgctatc accagcagct taaacagcag
     4681 ctcgaccctt gcggcgtgtt taatcccggt cgcatgtacg cggagcttta aggagcaggc
     4741 tatgcaaacc caattaaccg aagagatgcg gcagaacgcg cgggcgctgg aagccgacag
     4801 catcctgcgc gcctgtgttc actgcggatt ttgtaccgca acctgcccaa cctatcagct
     4861 cctgggcgat gaactggacg ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga
     4921 aggcaacgac gtcacgctta aaacacagga gcatctcgat cgctgtctca cttgccgcaa
     4981 ttgtgaaacc acctgtcctt ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga
     5041 tattgtcgag cagaaagtga aacgcccact gccggagcga atgctgcgcg aaggattgcg
     5101 ccaggtagtg ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc tggtgctgcg
     5161 accgttttta ccggaacagg tcagagcaaa actgcctgct gaaacggtga aagctaaacc
     5221 gcgtccaccg ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg cccagcctac
     5281 gctttcgccc aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt
     5341 catgccagct aacgaagcag gctgttgtgg cgcggtggac tatcatctta atgcgcaaga
     5401 gaaagggtta gcgcgggcgc gcaataatat tgatgcctgg tggcccgcga ttgaagcagg
     5461 tgccgaggct attttgcaga ctgccagcgg ctgcggcgcg tttgtcaaag agtatgggca
     5521 gatgctgaaa aacgatgcgt tgtatgccga taaagcgcgt caggtcagtg agctggcggt
     5581 cgatttagtc gaacttctgc gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa
     5641 aaaactggcc ttccactgcc cgtgtaccct acaacatgcg caaaagctga acggcgaagt
     5701 ggaaaaagtg ttgcttcgtc ttggatttac cttaacggac gttcccgaca gccatctgtg
     5761 ctgcggttca gcgggaacat atgcgttaac gcatcccgat ctggcgcgcc agctgcggga
     5821 taacaaaatg aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg ccaacattgg
     5881 ttgccagacg catctggcga gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt
     5941 agaacaagcc cttgaaaagg aataacaaaa tgaaaactaa agtcattctt agccagcaaa
     6001 tggcgagtgc aattattgcc gcaggtcagg aagaggcgca gaaaaacaac tggtctgttt
     6061 ccattgctgt tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg
     6121 cgccgattgc ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg
     6181 aaactaaggg gtatgaagag atggtgaaca acggacgtac cgcgttcgtg actgcgccgt
     6241 tattaacgtc gctagaaggt ggcgtaccgg ttgttgtgga tgggcaaatt attggtgccg
     6301 tgggcgtttc tggtttaacc ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg
     6361 tgttggcgaa ataagcgaaa acgaggagag aaacaatgag tcaaaccata acccagggcc
     6421 gtttacgcat taacgccaat tttaaacgtt ttgtggatga agaagtttta ccgggaacag
     6481 ggttggacgc tgcggcgttc tggcgcaatt ttgatgagat cgttcacgat ctggcaccag
     6541 aaaatcgtca gttgctggca gaacgcgatc gcattcaggc ggcgcttgat gagtggcatc
     6601 gcagcaatcc ggggccggta aaagataaag cggcctataa atctttcctg cgtgaactgg
     6661 gctacctggt gccgcaaccg gagcgcgtaa cggtggaaac cacgggcatt gacagcgaaa
     6721 tcaccagcca ggcggggcca cagctggtgg ttccggcaat gaacgcccgc tacgcgctga
     6781 acgcggcgaa cgctcgctgg ggctcactgt acgatgcgtt atacggcagc gacatcatcc
     6841 cgcaggaagg ggcgatggtc agcggctacg atccgcagcg cggtgagcag gttatcgcct
     6901 gggttcggcg tttcctcgat gaatcactac cgctggaaaa cggcagctat caggatgtgg
     6961 tgacgtttaa ggtggttgat aaacaattac gcatccagtt gaaaaatggt aaagaaacca
     7021 cgttacgtac cctggcgcag tttgtcggtt accgtggcga tgccgctgcg ccgacctgca
     7081 ttttgctgaa aaataacggc ctgcatattg agctgcaaat tgatgccaac ggacggattg
     7141 gcaaagacga tccggcgcac atcaacgatg ttatcgtcga agctgccatc agtaccattc
     7201 tcgactgcga agattcggtc gcggcggttg atgcggaaga taaaatcctg ctgtaccgca
     7261 atctgctggg tctgatgcag gggactctgc aagagaaaat ggaaaaaaac ggtcggcaaa
     7321 tcgtgcgtaa actgaatgac gatcggcagt acaccgccgc cgatggctct gaaatttctc
     7381 tgcacggacg ctcgctgctg tttatccgca acgtggggca tttgatgacc attcctgtga
     7441 tttgggacag cgaaggcaat gaaatcccgg aaggcattct tgatggcgtc atgacaggcg
     7501 cgattgccct ctatgattta aaagtgcaga aaaactcgcg cactggcagc gtctatattg
     7561 tgaaaccgaa aatgcacggt ccgcaggaag tggcgttcgc caacaaactg tttacccgcg
     7621 ttgagacaat gctcggtatg gcaccgaata ccctgaaaat gggcattatg gatgaagaac
     7681 gccgaacctc gctgaacttg cgtagctgta ttgctcaggc gcgcaaccgc gtggcgttca
     7741 tcaataccgg tttcctcgac cgtaccggcg atgagatgca ttcggtgatg gaagctggcc
     7801 cgatgctgcg taaaaaccag atgaaatcga cgccgtggat caaagcctac gagcgtaata
     7861 acgtgctttc cggtctgttc tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt
     7921 gggcaatgcc ggacctgatg gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg
     7981 gggcaaacac agcctgggtt ccgtcaccaa ccgctgctac gctccatgcg ctgcactacc
     8041 accaaaccaa cgtacagagc gtgcaggcca acattgccca gagcgagttc aatgctgaat
     8101 ttgaaccgtt gctggacgat ctgctgacta ttccggtggc tgaaaacgct aactggtcgg
     8161 tggaagagat ccaacaagag ctggataaca acgtgcaggg gattctgggg tacgtggtgc
     8221 gttgggtgga gcaggggatt ggttgttcaa aagtgccgga tattcataac gtggcgctga
     8281 tggaagaccg cgcaacgctg cgtatctcca gccagcatat cgccaactgg ttacgtcacg
     8341 gtattctgac caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc
     8401 agcaaaacgc tggcgatccg gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg
     8461 cttttaaagc tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac ggctataccg
     8521 aaccgttatt acacgcctgg cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa
     8581 gccccggcaa gttttattgt cggggctatg atagtaactg cggtagtaca tttatcccgg
     8641 aaataaccgg agcgattatc agggtttaac aatattaaat acccgtagcg cattactagc
     8701 gggcctaaaa caacaatatg gacatcttaa cgatattctg cgcctgcgtg tgcggaacag
     8761 aaacacttac ccgatatact tgttatgcca gaggtagcaa ttgcgttagc agcgtactgt
     8821 tgctgtaaag tataacgcct atattttaca gttcagtcct cttttgctta ttacaacctt
     8881 aagtatatca agcatataaa gataataaga gactgaacaa tatggttacc tggacccaaa
     8941 tgtatatgcc gatgggagga ctggggctat ccgctctggt cgccctgatc ccgataatat
     9001 tcttcttc
//
LOCUS       DACADA010000171         8994 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.173, whole genome shotgun sequence.
ACCESSION   DACADA010000171 DACADA010000000
VERSION     DACADA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.173"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..316)
                     /locus_tag="ISB17_004041"
     CDS             complement(<1..316)
                     /locus_tag="ISB17_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981917.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFMGAPQKTESMV
                     TPV"
     gene            complement(345..1042)
                     /locus_tag="ISB17_004042"
                     /pseudo
     CDS             complement(345..1042)
                     /locus_tag="ISB17_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1104..1226)
                     /locus_tag="ISB17_004043"
     CDS             complement(1104..1226)
                     /locus_tag="ISB17_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981918.1"
                     /translation="MRFRFSLSGTWLTICNIIIKYNYASYFYSQNANNVAYTPV"
     gene            complement(1298..2320)
                     /locus_tag="ISB17_004044"
     CDS             complement(1298..2320)
                     /locus_tag="ISB17_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HAP1981919.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLSEDRRTLLLTFTVNNEAHLRAQ
                     IIRRFGFIPEGIRVMTWFEFLHGFCFRPFLQEQLASRGLSFDQPPPRIPRTNARHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRIRLARYYDELLVDEVQDFAGHDFNFLLELCRAEI
                     TVLCCGDFYQHTFDTSHDGNVNSTLHDDITRYEARFDAAGFAVDRDTLNRTWRCSASV
                     CEFITGQLNIRIAAHGIHASLIETIADTERSATLHADNTVIKLFYREHHRYGCYSMNW
                     GASKGLDHFQDVCIVMGSSHWKLLTRQELATLPPSSRNRLYVACSRARGNIYFVPETH
                     LRRFRN"
     gene            complement(2305..3867)
                     /locus_tag="ISB17_004045"
     CDS             complement(2305..3867)
                     /locus_tag="ISB17_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1981920.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFTHDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRQFQEGERRADQLPVLTADVFLSNGGDPDLNGR
                     QNLAGINADGLRMRIAPMTEEYGQDIHNVLQQDPDNFPYEYYSVRFSTFSGGHFASFR
                     RYLRHLLLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCTRHLSAI
                     NDTLETYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGEL
                     HVLLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGSARP
                     VLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYRRLYGRAPEED
                     GVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVICSRSRVFADR
                     DNTRSTFEISLYQDNADLCDTLFRGPRRTLTVQEYMLANKAEAAFRLLQLHAGELTVP
                     DYIQEALAWIRE"
     gene            complement(3941..4357)
                     /locus_tag="ISB17_004046"
     CDS             complement(3941..4357)
                     /locus_tag="ISB17_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HAP1981921.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITY
                     SEMRFGATGPKASPRHVELVDAFCARLDAILPWDRAAVDATTEIKVALRMAGTPIGPN
                     DTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR"
     gene            complement(4354..4584)
                     /locus_tag="ISB17_004047"
     CDS             complement(4354..4584)
                     /locus_tag="ISB17_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAP1981922.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFAQFEKADPDFMAEREDVVSDEGRFNL"
     gene            4541..5002
                     /locus_tag="ISB17_004048"
     CDS             4541..5002
                     /locus_tag="ISB17_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981923.1"
                     /translation="MARLLPFLKMDTVLIISSWPTGLYDYVQYMQMQSICIVIAYADG
                     KYMRMPHLCRCPAYGYVEPMLYSLFSRSDPKKASSGSAFRPPVRSGPETQDVAVLPAE
                     DDAICCVPPLPRDRPLTTGKRRFAYSLTVVHFRKLRKARKVGSGRNGRPVI"
     gene            5172..5390
                     /gene="ccdA"
                     /locus_tag="ISB17_004049"
     CDS             5172..5390
                     /gene="ccdA"
                     /locus_tag="ISB17_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAP1981924.1"
                     /translation="MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            5392..5697
                     /gene="ccdB"
                     /locus_tag="ISB17_004050"
     CDS             5392..5697
                     /gene="ccdB"
                     /locus_tag="ISB17_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAP1981925.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEVADLSHRENDIKNAINLMFWGI"
     gene            5866..6261
                     /locus_tag="ISB17_004051"
     CDS             5866..6261
                     /locus_tag="ISB17_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981926.1"
                     /translation="MNRTDGEPETDVMATAFSTMRKYGMNIITATQHMDINAYVKSDP
                     RMPRTRLRRYLGQYGLRGEALSAEVRRIVAGPERPDNTLKAGIFYTWPYGWEPADFTP
                     VMRRIFKGKRTRHVMLFDELPQLFGKNDY"
     gene            6288..6602
                     /locus_tag="ISB17_004052"
     CDS             6288..6602
                     /locus_tag="ISB17_004052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981927.1"
                     /translation="MGTPTFSSFNDVVRELEDVYGHQELWLYSGLNEDSPIETARRRQ
                     KWRSPKILKRNGRMVAEQSGQPDFWVLTGDYHLPQSEHSAPPWKACLINKVFKVYCSL
                     HC"
     gene            6613..7629
                     /locus_tag="ISB17_004053"
     CDS             6613..7629
                     /locus_tag="ISB17_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981928.1"
                     /translation="MTDCVTRTGDDKTMWLVTLPEIITNNSTRQEENLVVILSREESW
                     GPTSDHFPDGLYRVSCIMTLYLADTELTKTQTFTAIYWPQQKALHLFTDEFRLERRLQ
                     GLGLGSWITQQFVLWARGLPPATLVLPIEISRVDEENEENKIRRDRLWHAMGFRFPAG
                     ETSSMPLRADELQLPRGRCSTLRVEPLVSAVRRLEDCYRGLQEKIVQLEGKKRSQKQL
                     ITSLKNRPFYHLLHRKEGQLPDEKCDALPLRAEKLSLPQSGDSDLEVAQRATGVIRLA
                     TLCAQSQKRILELERELASQSEELVDLQAHPFFNLRDKYRGQILFWGWGMGFLCFIYW
                     MLVS"
     gene            7673..7818
                     /locus_tag="ISB17_004054"
                     /pseudo
     CDS             7673..7818
                     /locus_tag="ISB17_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7827..8621
                     /locus_tag="ISB17_004055"
     CDS             7827..8621
                     /locus_tag="ISB17_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP1981929.1"
                     /translation="MNNLVPVGSPASPGTLLPVAIDYPAALALRQMALVQDELPKYLL
                     APEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLVPPYPFVQLATLKQR
                     TEKAARTAGRAPAGSQVHRLVPLSDHHYVSQLQMMVATLKIPLERRNKRTGRMEKARI
                     WEITDRTVRTWLSEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEAEAVALIKKLTPNQST"
BASE COUNT         2136 a         2388 c         2429 g         2041 t
ORIGIN      
        1 aaacgggagt gaccatagat tccgttttct gaggtgcccc cataaagccc tgcgcagcgc
       61 gcagggtcag cctgaatacg cgtttaatga ccagcacagt cgtgatggca aggtcagaat
      121 agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatagctt
      181 catcatccag ccagaaagtt atggagccac ggttgatgag ggctttattg taggtgggcc
      241 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatacg
      301 tgatctgatc cttcaactca gcaaaagttg gtaatgactc caatttactg atagtgtttt
      361 atgttcagat agtgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagg
      421 gacttcctgc ccagccttgc cagatgctgt ctcagattca ggttgtgtcg ctcaattcgc
      481 tgcgtgtaac gcttactgat gacgtgcagt tttcccttca gacgtgactc atacatcggc
      541 cagccgtccg tcatccatac caccacgtca aaaactgaca gcaggctcag aagacgctcc
      601 agtgtggcca tagtgcgttc accgaaaacg tgggctacca ccgccctgcg tatcctgtca
      661 tacgcgtaaa acagccagcg ctgacgtgat tttgcaccga cgtagcccca ttgctcgtcc
      721 atctccgcac agacaatgac atcactgccc ggctgtatcc gggaggttac tgactgcggc
      781 ctgagttttt taaatggcgc aaaatggtgt tgaggcccac gcccattagt cgtgcggtgg
      841 cacggcaacc gacgccgttc atagccatat caatgatttt ctggtgtgta ccgggctgag
      901 aagcggcata agtgaaggtg agctgccatg ttttacggca gtgagagcag agataacgtt
      961 gatgcccggc agtacttttg ccattacgca tcacgccttc agtagcggaa caggagggac
     1021 agcagacgga gactgaagcc acgggagcac ctcaaaaaca ccattataca ctaaatcagc
     1081 aagttggtag catcaccatg ttttcatact ggcgtgtatg ccacattatt agcattttgc
     1141 gaataaaaat aactggcata attgtattta atgataatgt tacatattgt tagccaggtt
     1201 ccggaaagcg agaaccggaa ccgcacctgt ccagccggta ttcccgtcga tgaccctcgc
     1261 tttttactga tttgtgcccg actgagaagt cggtaactca gtttcggaat cggcgcagat
     1321 gagtttcggg aacaaaataa atgttgccgc gcgcccgtga gcatgccacg tacagcctgt
     1381 tacgggacga cggtggaagg gttgccagtt cctgacgggt cagaagcttc cagtgggagg
     1441 atcccatcac aatgcagaca tcctgaaagt gatcgagccc tttactggcg ccccagttca
     1501 tggaatagca gccgtaacgg tgatgttcac gatagaagag cttaatcacc gtattatcgg
     1561 cgtgcagggt ggctgagcgc tctgtatctg cgatcgtttc aatcagggag gcatgtatcc
     1621 catgggcagc gatacggatg ttcagttgcc cggtgataaa ctcgcacact gaagctgagc
     1681 aacgccatgt ccggttgagc gtgtcccggt caaccgcgaa acctgctgcg tcgaagcggg
     1741 cctcatatcg cgtgatgtcg tcgtgcagcg ttgaattaac gttgccgtca tggcttgtat
     1801 caaaggtatg ctgataaaaa tcgccgcaac agagcacagt gatttccgcg cggcagagct
     1861 ccagcagaaa attaaagtca tgtccggcaa agtcctggac ttcatcgaca agcagttcat
     1921 catagtaacg ggcaagccgg atacggatat ccggcagcaa cccccgggct gtcagtaaat
     1981 gtgcgagcct ccggtgatac agccgtccgg cgggatcctg ataatgccgg gcattcgtgc
     2041 gcggtattct gggtgggggc tggtcgaagc tcaggccacg cgatgccagc tgctcctgca
     2101 ggaaaggccg gaagcaaaaa ccgtgcagaa actcaaacca ggtcatcacc ctgatccctt
     2161 ccgggataaa gccaaaacgt ctgataatct gtgcccgcag atgcgcttca ttattcacgg
     2221 tgaaggtcag gagcagcgtc ctcctgtcct cactcaaccg tcgtatcagc agcgtggtct
     2281 tgcccgagcc ggcaaccgca aatatcactc tcttatccat gccagtgcct cctgaatata
     2341 atcaggtacg gtcagctctc cggcgtgcag ctgtaacagc cggaatgctg cttctgcctt
     2401 attggccagc atgtattcct gcaccgtgag tgtccggcgg ggtccgcgga aaagcgtatc
     2461 acacagatca gcgttatcct gatacaggct gatttcaaat gtggatcggg tgttatcgcg
     2521 atcagcaaat acccgggagc gggaacaaat cacgtcagca tagcgttcat cgcagttttg
     2581 ctgatagttg ccgtcattat cacgaagggc cgcgacgcga ttttccagaa gacgggccag
     2641 ttcaagataa cgacggaaac tcgttccgcc gatagcgatg atgtgaactc cgtcttcctc
     2701 cggtgccctg ccatacaggc ggcgataaaa ggcctcgatc agaataaact cagcatcccc
     2761 ctccacgagc agcacacgtc tggccagcgc aaattccagt acgttattat ccggtgcttt
     2821 catgaagaag gcggcagtct cagcggaaag ttcattcatc agtaccggcc gggcagaccc
     2881 gaggaggatt gccttacgca gatcgaggcg ggaggagata tggctgctgt gtgtggcaat
     2941 aaacacctgc gtctgccgtt cagtggcaag ctgattaacc agcctcttca tactgacatg
     3001 gctgagatga ttttccggct cctcaagcaa cagtacgtgg agctctccct gctgctgatg
     3061 ccgctgcagc gcaaactccg ttttgatgaa gcactgcctt ccttttccac ggtgacgaat
     3121 ggaaataccg tcctcggtaa tgtccagatt cgcctcaagc cctgatttag caccggaacg
     3181 cacgccaaac tgatacgttt ccagcgtgtc gtttatggct gacagatgcc gggtgcagaa
     3241 gtgcattttc tgttgccggt aactgttttc aagccggtac cgatcggcca ccggcacatt
     3301 gacgctgtaa actgtgcgcg tatattcctg tgcggcgtgc tcgttatcga tgcgtgcact
     3361 gtctaagagc agatggcgca gatagcgtct gaagctggcg aaatgaccac cggagaaggt
     3421 gctgaaccgc acggaatagt attcataggg aaaattatcg ggatcctgct gtagtacatt
     3481 gtgaatatcc tggccgtatt cctccgtcat tggtgcaata cgcattctga gaccatccgc
     3541 attgataccc gccagatttt gcctgccgtt caggtcagga tctcccccat tactcaggaa
     3601 aacgtcagct gtcagcacgg gtaactgatc ggcgcggcgc tcaccttcct gaaactgcct
     3661 tactgctgac tgggacagga gcgattccac acccaatgcc tcgacccggt gccggctgtc
     3721 actcagcaca aggtcaaggg ccagcagaat cgtgcttttc ccggactcat tgtccccgac
     3781 cagaatgtta cggtcatgag tgaagcgcag gtccagttcc ggaaactttt taaagttttg
     3841 cagcataagc cgcgtgatca gaggcatctt ttattccttt atataccgct gactgaaaga
     3901 gtgtccggct gcgccagacc gcctgttttg ttttacagcg ttatcttacc cagtcttcca
     3961 gcacaagacc cggtacccgc tcaaattccc gcgtattatt cgtcaccagc acggcaccgg
     4021 cggcgatggc gtgcccggca atggccgtgt cgttcgggcc gatcggcgtc ccggccattc
     4081 gcagcgccac cttaatctcc gtggtggcgt ccaccgcagc gcgatcccag ggcaggatgg
     4141 catcgaggcg ggcgcagaac gcgtcgacca gctcgacgtg gcgcggggag gccttcgggc
     4201 cggtggcccc gaagcgcatt tcagaataag tgatggccga gacaacgatg cgctggccgc
     4261 gcagcaccgc ctgctccagg cgcttcagga cggcttccgg ctgctcgcgc atgatgaacg
     4321 agcagataca ggtgtcgagc atataggtct tgttcacagg ttaaatcgtc cttcgtcgct
     4381 gacaacgtcc tcgcgctccg ccataaagtc cgggtctgcc ttctcgaact gcgcgaacga
     4441 gccccaggtc ggccggacgg gacgcaaaat aatgctgtcc ccttcccgga cgatctccag
     4501 ctcgctcact ccctcaaaat ccagatcgcg gggcagacga atggcgcggt tattgccgtt
     4561 tttaaaaatg gatacagttc tcatcatttc ctcctggccg accggcctgt atgactatgt
     4621 tcagtatatg cagatgcaaa gcatatgtat agtcatagca tatgcagatg gtaagtatat
     4681 gcgtatgcca cacctatgca gatgcccggc atatggatat gttgaaccta tgttgtattc
     4741 tctcttttcc cggtcagatc cgaaaaaagc tagttccgga tctgctttcc ggcctccggt
     4801 gcgcagcggc ccggaaacac aagatgtagc ggtgttacca gctgaggatg acgctatatg
     4861 ttgtgtccct cccctgcccc gcgaccggcc gctcaccacc ggaaaacgcc gctttgctta
     4921 ttctctcacc gtggtacact tccggaaact tcgtaaagcg cgaaaagtcg gatccggacg
     4981 gaacggccgt cctgtaattt aacggcgtgg ctgtatggct gcttgttatc tctaaggtga
     5041 ttattttgta tgtgaatttc acgaagtgaa attaatggcg acataacagt atgtttcagg
     5101 cgggcgtgag tcagctgcat ccttacgcta tactgatatg catatcatca gtatgtattc
     5161 cggagtgcat tatgaagcag cgcatcactg tcaccataga cagcgacagt tatcagttac
     5221 tgaaatccgc agacgtcaat atctccgccc tggtgaacag ggccatgcag aatgaggccc
     5281 gtcgtctgcg ttccgaacgc tggcaggctg aaaatcagga agggatggcc gaggttgccc
     5341 gctttattga gatgaacggt tcattcgccg acgaaaacag ggactggtga gatgcagttt
     5401 aaggtttatg cctataaaag agagagccgc tatcgtctgt tcgtggatgt gcagagtgac
     5461 atcatcgata cccccgggcg acggatggtg atcccgctgg ccagcgcccg gctgctgtcg
     5521 gataaggttt cccgtgaact ctatccggtg gtgcacatcg gggatgaaag ctaccgcctg
     5581 atgaccacgg acatggccag cgtcacgtcc tccgtcaccg gggaggaagt ggccgatctc
     5641 agccaccggg aaaatgacat caaaaatgcg ataaacctga tgttctgggg aatttgacag
     5701 cctccccgcc ctgaaagaca tcgaggatgt caaaacgaag cattgccgca gagcttaacc
     5761 ctcagcatga actgccctcc ggagcggatg ccctgcaggc tttcgcgggg tcagcttaaa
     5821 cgaacggttc acacaaacac ccttaagtca gtaaaaggaa accagatgaa cagaactgat
     5881 ggcgaaccag agacagacgt gatggccacc gctttcagca ccatgcgcaa gtacggcatg
     5941 aatatcatca ccgccacgca gcatatggat attaatgctt acgtgaaatc cgatccgcgg
     6001 atgccgagaa cgcggctgcg tcgttacctg ggacagtacg gcctgcgcgg tgaggccctg
     6061 agcgcggagg taagacgtat cgtcgctggc cctgaacgac cagataacac gctgaaagcc
     6121 ggaatcttct atacgtggcc ctatggctgg gagcctgcgg atttcacgcc agtgatgagg
     6181 cgcattttca agggaaagcg cacccgccat gtgatgttgt ttgatgaact gcctcagctg
     6241 tttggcaaga atgactattg aaccggatac tatttttgag gcgaggtatg ggtacaccaa
     6301 catttagctc atttaacgat gtggtccggg agcttgagga tgtatatggc caccaggagc
     6361 tgtggcttta ttctggcctt aatgaggaca gccccatcga gacagcccgc aggcgtcaga
     6421 aatggcgatc tccaaaaatt ctcaaacgaa acggcagaat ggtggcagaa caatcaggcc
     6481 agcctgattt ctgggtgctc acgggtgact accatctgcc gcagtctgaa cacagcgctc
     6541 cgccgtggaa agcctgcctc atcaataagg tctttaaagt gtattgttca cttcactgtt
     6601 agggaaaaaa acatgaccga ctgtgttacc cggactggtg acgacaaaac aatgtggctc
     6661 gtgacattgc cggagatcat tacaaataac agtacccgcc aggaggaaaa tctggtcgtt
     6721 attctgagca gggaggaatc gtgggggcca acttctgacc attttcccga cggattgtac
     6781 cgggtctcct gcataatgac actgtacctt gccgataccg aactgacgaa gactcagact
     6841 ttcactgcaa tctactggcc tcaacagaag gccctgcatc tgtttacaga tgagttcaga
     6901 ctggaacgcc gcctgcaggg actcggcctg ggtagctgga tcacgcagca gtttgttctc
     6961 tgggccagag gccttccgcc agcaaccctg gttttgccga ttgaaatttc ccgggttgat
     7021 gaagaaaacg aagaaaacaa gatccggagg gacaggctgt ggcacgcaat gggtttccgg
     7081 ttcccggcgg gggaaaccag ctcaatgccg ctgcgggcag acgaactgca gttgccacgg
     7141 ggcaggtgct caacgctccg ggtggagccc ctggtatccg cggtacgccg gctggaagac
     7201 tgttatcgcg ggctgcagga aaaaatcgtg cagctcgaag gcaaaaaacg gtcgcaaaaa
     7261 cagttaatta cttcacttaa gaatcgtcca ttttatcatc tgttgcacag gaaagagggc
     7321 cagctcccgg acgagaaatg tgacgccctg ccgctgcggg cagaaaaact gtctctgccg
     7381 cagagcgggg attcagacct ggaggtggcg caacgggcaa ccggggtcat ccggctggct
     7441 accctgtgtg ctcagtcgca gaaaagaatc ctggagctgg aaagggagct tgcctcgcaa
     7501 agtgaggagc tcgttgacct gcaggcacat ccctttttta acctgcggga taaataccgc
     7561 ggccagatcc tgttctgggg atgggggatg ggttttctgt gctttatcta ctggatgttg
     7621 gtgtcatgaa tgttcccgtt ccatagccac ccactgaaga ggagtgaaac ctgtgcccga
     7681 aaatatcgtg tctaccgtca ttgacggggt agttttgttt gtgacttcgt ctgttttctg
     7741 gctggccgtc gctggctggt tagtcatcct gactccggta atattacgaa gaaaattctc
     7801 ggcaaagaaa aaaattaaca gaaattatga ataacctggt tccagttgga tctccggcat
     7861 ctcccggaac attgctcccg gtggccatcg attaccctgc cgccctcgcc ctgcgccaga
     7921 tggcgctcgt gcaggatgaa ctgccgaaat acctgctggc gccggaagtc agcgccctgc
     7981 tccattacgt gccggatctt caccgcaaga tgctgctggc gacgctgtgg aacaccggcg
     8041 cgcgcattaa tgaagcgctc gcgctgaccc ggggggattt ttcgctggtg ccgccgtatc
     8101 cgttcgtgca gctggccacg ctcaagcagc gcaccgagaa agcggccagg acggcaggtc
     8161 gtgcccccgc aggcagtcag gtccatcgtc tggttcccct ttccgatcac cactatgtca
     8221 gccagctgca gatgatggtg gccaccctga aaattccgct ggaaagacga aacaagcgca
     8281 ccggcagaat ggagaaggca cgtatctggg agatcacaga ccgtaccgtg cgcacctggc
     8341 ttagcgaagc ggtggaggcc gcagcggccg acggggtgac gttctcagta ccggttaccc
     8401 cccatacgtt ccgtcactcc tacgcgatgc atatgctgta cgccggcata cctctgaagg
     8461 ttttgcagag cctgatgggc cacaaatcga tcagttccac ggaggtctat acgaaggtgt
     8521 ttgcgctcga tgtcgcggca cggcaccggg tgcagtttgc tatgccggaa gctgaagctg
     8581 ttgcgttgat aaaaaagtta accccaaacc aatctacata ggtcaactat gggtaatacc
     8641 ccaggtttcg acctaaagta gttcttgtgt gggaaacagt ccaagcttaa tgggggctta
     8701 tccagcctca ctttttacag ctttcaatca cattaattat gttcagaaag gccctcattg
     8761 ccctaaagaa aaaaaatgca gccatgaaaa taaggaataa ttgagataca actcctgcta
     8821 aatcatggtt gctaccagcc actgatagca acgtaaatag taatgcaagc gaaaaatgta
     8881 aggcgattgt agtatttgaa ttcatgtata tccccttatg tataggactg aggccatccg
     8941 caaattttca ctatcgcgaa gttgttgcct cgatataaaa ggtgttacca ccct
//
LOCUS       DACADA010000172         8871 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.174, whole genome shotgun sequence.
ACCESSION   DACADA010000172 DACADA010000000
VERSION     DACADA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.174"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..166
                     /locus_tag="ISB17_004056"
     CDS             <1..166
                     /locus_tag="ISB17_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1981930.1"
                     /translation="YTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRI
                     GFNFSSIEDG"
     gene            543..800
                     /locus_tag="ISB17_004057"
     CDS             543..800
                     /locus_tag="ISB17_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981931.1"
                     /translation="MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRAQLDNR"
     gene            812..1363
                     /locus_tag="ISB17_004058"
     CDS             812..1363
                     /locus_tag="ISB17_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1981932.1"
                     /translation="MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDES
                     ARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
                     QPMNVTGHIQCAQALMKPEHWRE"
     gene            1415..2161
                     /locus_tag="ISB17_004059"
     CDS             1415..2161
                     /locus_tag="ISB17_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1981933.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPETRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQARRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGTVELLAQSLKPGGIMLIGEPYWRQLPATDEIAQACGVSS
                     TTDFLTLPGLVSAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNISPKRHVTYAREYFGWGVFALIAR"
     gene            2289..2549
                     /locus_tag="ISB17_004060"
     CDS             2289..2549
                     /locus_tag="ISB17_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981934.1"
                     /translation="MSQHEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            2948..4069
                     /locus_tag="ISB17_004061"
     CDS             2948..4069
                     /locus_tag="ISB17_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP1981935.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            4066..4344
                     /locus_tag="ISB17_004062"
     CDS             4066..4344
                     /locus_tag="ISB17_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP1981936.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            4356..5669
                     /locus_tag="ISB17_004063"
     CDS             4356..5669
                     /locus_tag="ISB17_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1981937.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            5682..6488
                     /gene="sgcQ"
                     /locus_tag="ISB17_004064"
     CDS             5682..6488
                     /gene="sgcQ"
                     /locus_tag="ISB17_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HAP1981938.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            6619..7050
                     /locus_tag="ISB17_004065"
     CDS             6619..7050
                     /locus_tag="ISB17_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1981939.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            7062..7694
                     /locus_tag="ISB17_004066"
     CDS             7062..7694
                     /locus_tag="ISB17_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HAP1981940.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            7711..8493
                     /locus_tag="ISB17_004067"
     CDS             7711..8493
                     /locus_tag="ISB17_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1981941.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
BASE COUNT         2137 a         2268 c         2281 g         2185 t
ORIGIN      
        1 ttatactcgc cagggatatg caattgaagc aattaccgcc acgattgact ggataaaaga
       61 gaacggtttt tcactcatca aagccggtgt gaatccggaa aatacccttt caaaaaaatt
      121 acttatcagg attggcttca atttttcgag catcgaagat gggtgagcat atttatgttc
      181 tggattaaac aaaaggagaa taatctcccg aagggaaaag ccgatgaggc catttcaggc
      241 gtacttcaac atgcattcgt cagtgtcatc tgccttttcc agtaaatggg ttggcattca
      301 atttccaaag tacatcacac tttttctact gaaacatatc cctcctataa ctacgtgtta
      361 atattcgctc cgtaaatacc tttcaactca acccgaggct ttgatgctgg agaatgtaat
      421 catccgataa tcagttcccc gaccttttca ggccggactg attatcaatg cgccgaaatc
      481 gaatgcggac accgcggtgt gtttgcacgt tttgcacatg atgattaaac aggttcttca
      541 gtatgaattt atcccgtcag gaacaacgta cgttacacgt tctcgctaaa ggtggacgta
      601 ttgcgcacgt ccgcgattct tcaggccgcg tcacttccgt tgaatgctac agccgcgaag
      661 ggctgttgct gaccgactgc acgctcgccg tcttcaaaaa actcaaaacc aaaaaactta
      721 tcaagtccgt caatggtcag ccctatcgaa tcaacactac cgggctgaat aacgttcgcg
      781 ctcagctcga taatcgctaa ggagagtttc gatgacagca caccacttca cttttcaaat
      841 cacagatgaa agtgatgcca gtgatatccg ggaagtcgaa acccgcgcat ttggcttcag
      901 caaagaagcc gatttaacgg cctctttact tgaggatgaa agcgcgcgcc ctgcgttatc
      961 gctgctggcc cgacatgaag gcaaagccgt cgggcatatt ttatttaccc gggcaacctt
     1021 caaaggggaa atggattcac cgctgatgca tatcctcgct cccctggcag tcataccgga
     1081 ataccagggg atgggcgtgg gaggacggtt gatacgcact ggcatcgagc acctgaggtt
     1141 gatgggctgc cagacggttt ttgttctcgg ccacgccacc tattaccccc gacatgggtt
     1201 cgaaccctgt gccggagaca aaggctatcc ggcaccttat cccatcccgg aggagcacaa
     1261 agcctgctgg atgatgcaag cactcaccgc tcagcccatg aacgtgaccg gccacatcca
     1321 gtgcgcgcag gccctgatga aaccggagca ctggcgtgaa taacaatctt gttgtgccct
     1381 ttttaatgct taacccaata acaggaatta actaatggat attccacgta tttttaccat
     1441 cagtgaaagc gaacaccgca tccacaaccc gttcaccgaa gagaagtacg ccacgctggg
     1501 tcgcgtgctg cgcatgaaac cagagacccg aattctggac ctcggcagcg gctcgggcga
     1561 gatgctctgc acctgggccc gggatcatgg cattacgggg actggaatcg acatgagttc
     1621 gctcttcacc gcgcaggcca gacgccgcgc ggaagaactc ggcgtcagcg aacgcgtaca
     1681 ttttattcat aatgatgcgg cgggttacgt cgccaatgaa aaatgtgacg tagcagcctg
     1741 cgttggcgcg acctggattg ccggaggctt tgccgggaca gtcgaactgc tggcgcagag
     1801 ccttaaaccg ggcgggatca tgctcatcgg tgaaccttac tggcgtcagc ttccggcaac
     1861 ggacgagata gcccaggcct gtggcgtcag ctcgacgacc gattttctga cgctaccagg
     1921 gcttgtcagc gcgtttgacg acctcggcta cgacgtggtg gaaatggtac tggcggacca
     1981 ggaaggctgg gaccgatatg aggccgcgaa atggctgacc atgcgccgct ggctggaggc
     2041 caatcctgac gatgacttcg ccgccgaagt cagggccgag ctaaacatct cgccgaaacg
     2101 ccacgtaacg tacgcgcggg aatactttgg ctggggcgtg ttcgcactca tcgctaggta
     2161 acaacaggtt cccggtattt ctgactggag ataccggggg cttagccttt gctttaatct
     2221 gatgcatcac ctgcacaata catattcagt atgtttatta atcaagatgt caccataagg
     2281 agcatcatat gtcacaacat gaacagttct gtcaggcttg cggaatgccg atgtcggcac
     2341 cagatgcaca cagtgccagt gatcaatatt gtgcgtactg cagcgacagc aacggcaatc
     2401 tcaaaccctg ggaagaagcg gtatcaggtt tagccagctt ccttgattcc tggcaaaaag
     2461 tcggcccgga ggaagcacat aagcgtgcaa aacgttatct gacggcaatg ccagcctggg
     2521 ctcataaagc ggagagtgat ttgagctgat tatctatact ccctctgaat ctattcagga
     2581 gatccgatgt tttcttacat tatgctggga accaacgacc tgccacgcgc cattacattc
     2641 taagatccgc tgatggagat gctggggtat ccacgtgcag gacgtaatga agagggagcg
     2701 tcctgcaaca aagtggtgcg ataacagtta ccgcgtttta ttcattctca ttatttcaac
     2761 cataatcact ggtctctcta gcccgtaact ttcacaaacg ttgagcgctt ttattccact
     2821 cggctcacat aattttttat ttgttcagct ttgcgcgttt tcacgtgaac gttctcacaa
     2881 ttattcgacc agtctgcgcc tataaaacgc tcaacgagtg attgttttac ggtctacgga
     2941 gatagctatg tcattttctg tgcaggaaac cctgttctcg cttttgcagc acaatgcgat
     3001 ttcaggacac gaaaacgctg tcgctgacgt catgctgtgc gaattcaggc gtcaggcaaa
     3061 agaggtctgg cgagacaggc ttgggaatgt cgtcgcgcgc tacggtagtg ataaacccga
     3121 tgcgctgcga ctgatgattt ttgcgcatat ggatgaagtc ggttttatgg tgcgcaaaat
     3181 agagccttct ggatttttac gctttgaacg cgtaggcggt cctgcgcagg tcactatggc
     3241 tggatccatc gtcaccctca ccggggacaa agggccagtc atggggtgta tcggcattaa
     3301 gtcctaccac tttgccaaag gcgacgagcg cacgcagtca ccttctgtcg acaaactgtg
     3361 gattgatatt ggtgccaaag acaaagacga cgctatacga atgggcattc aggtcggtac
     3421 gcctgtcact ctgtataacc cgccgcaact cctggcaaac gatctggtgt gcagtaaagc
     3481 actagacgat cgtctgggct gtactgccct gctcggtgta gcggatgcta tcagtactac
     3541 ggagcttgat atcgccgttt atctggtggc ttcggtacag gaagaattta atatccgcgg
     3601 cattgttccc gtattacgcc gtgtaaaacc tgacctggcg attggtattg atatcactcc
     3661 atcgtgtgac acccctgatt tacacgatta ttccgaggtc aggattaatc agggcgttgg
     3721 gatcacctgc ctgaactacc atggtcgggg aacgctggcc ggattaatca cgcctcctcg
     3781 cctgatacgg atgttggaac agacggctct tgaacacaac attccggtgc agcgagaagt
     3841 ggctcccggc gtgataacag aaaccggtta tatccaggtt gagcaggatg gtattccctg
     3901 cgccagtctc tctattcctt gtcgctatac ccattctccg gcggaggttg ccagcctgcg
     3961 tgatttgact gattgcatcc gcctattgac cgctctggca ggtatgtcag cagcacattt
     4021 ccccgttgag cctgattcag gcactacaca agaggcacat ccattatgaa aaagatcctt
     4081 gtggcatgcg gtaccggcat gtcgacttca accatgattg cacacaagct gcaagagttc
     4141 ctgactgaac agggcatttc agccactacc gcccaatgct gtctgaatga gatccctctt
     4201 aactgtaacg gtatggacct tattgtaacc tccatgcgaa ccaacagtga ttacggcatc
     4261 cctacgctta atggcgccgc cctgctcaca gggatcaacg acgacgcgtt aaaacaacaa
     4321 atcaaggctc tgttaactca ataaaaggag gcattatgtt tgattacatc ctgtctctcg
     4381 gcggtaccgt tttcgtccct atcatcatga tcgtgattgg ccttatcttc cgcatcccct
     4441 ggctgcaggc gattaaggca ggggtaacgg ttgggattgg ttttgtcggt atgggactgg
     4501 tgatcgtcat ggcgatcgac agcctcagtc cccctatcaa agtgatgatt gagcgtttcg
     4561 gcctggccct gcatgtcttt gacgttggcg caggccctgc ctccggcgtt ggttatgcga
     4621 cagccattgg cgccatgatt atcccagtta tttttcttct caatgtcgcc atgctggtca
     4681 cccgtctgac caaaacgatg aacgtcgata tttacaatta ctggcactac gccattacgg
     4741 gtacggtggt ccagcttatg accggtagcc tgatttatgg tgtgctgggg gcaatctgcc
     4801 atgcggcgct atcgttaaaa atggccgact ggacggcaaa acgggtacag aacatcgtcg
     4861 ggctggaagg tatttcgata ccgcaaggct acggttccag ttcagtaccg cttttcgttc
     4921 tacttgatgc tatctatgaa aaaatccctt tcatgaaggg acgcaatatc gatgctcagg
     4981 agatccagaa acgctacggc atggtcggcg atccggtgat tatcggcgta gtgctcggtc
     5041 tgatttttgg cctggcggct ggtgaaggct ttaaaggttg cgccagtctg atgattaccg
     5101 ttgcggcaat catggtgctg ttcccgcgca tgatccgcct gattgttgag ggcctgatgc
     5161 ccatctccga cggtgcaaga aagttcttcc agaaatattt caaaggacgt gaagtgtata
     5221 tcggtctgga tactgctgtg acgctggggc atccaaccac cattgctgtc ggtctgctac
     5281 tgatacccat tatgttaatt ctggccagca ttttaccggg caacaaagtg ttacccctcg
     5341 cagacctgcc ggtagcaccg ttctttatct gtatggctac ggttatccac cgcggagacc
     5401 tggtcaggac gttgataagc ggggtcatcg tgatgattac cgtcctgctg atcgccaccc
     5461 agttcgcgcc ttacttcact gaaatggcgc tcaaaggcgg tttcagtttt gccggtgaaa
     5521 gtgcgcaaat ctcagcgttg tccgtgggca atatgttcgg ttggtcgatt tctgaattga
     5581 tgtcgcttgg gattattggc gttgtcgtgg cggtcggtat tgtcgccagc gtcgtgctgt
     5641 tcttacgtaa acgtgaatta tcggaataag gagtactttc aatgagttgg ctgaaagagg
     5701 ttattgggac ggaaaaagcg gtcatcgcca tgtgccatct acgtgcattg cccggcgacc
     5761 cgagttttga tgcgcagttg gggatgaact gggttatcga caaagcctgg gacgatctga
     5821 tggcgctcca aaatggtggg gtggatgcag tcatgttctc caacgagttt agtcttccct
     5881 atctcacgaa ggtgcgtccg gaaaccactg cggcaatggc acgaatcatc gggcaattaa
     5941 tgagcgatat ccgcatcccg tttggcgtga atgttctgtg ggatccggtc gcctcattcg
     6001 acctggcgat ggctaccggc gctaagttta tccgcgagat atttaccggt gcctatgcca
     6061 gcgactttgg cgtctgggac actaacgtcg gtgagaccat ccgtcatcaa caccggattg
     6121 gtgcaggcga agtgaaaacc ttgttcaata tcgtcccaga ggccgccgtg tatctgggca
     6181 atcgcgatat ctgttctatt gctaaatcga cagtatttaa caaccatcct gatgcgctct
     6241 gtgtttctgg cctgaccgcc gggacgcgta cagacagcgc gctgttaaaa cgggtcaaag
     6301 aaacggtgcc tgacaccgtc gtgttggcca acacgggcgt ctgcctggaa aacgtggagg
     6361 aacaactcag tatcgccgat ggctgtgtga ccgcaaccac ctttaaaaag gacggcgttt
     6421 ttgccaattt cgttgaccag gcgcgagtca gtcagtttat ggagaaagtg catcatatac
     6481 gccgataaga tagggataag cagagcgctt atccccggca aagagcaata acccttctgc
     6541 gatccctgcc gccttcgtca aaagtatcga cggggatcgc taccagatta acccgaatcg
     6601 gcatggagtc aacgacggat gattaacgat attaaatggg tacaggcaca acgtgaagcc
     6661 accgactggc gtcaggccgt ggaaatcgcc acccgcccgc tggtagccta tggtgccgcg
     6721 cagccatgct acgttaatgg gatcatcgaa aataccctta actgggggcc ttattatctg
     6781 atagccccag gaatcgcctt gcctcacgcc cgaccagagc aaggcgcaaa ctataatcag
     6841 gtcagtatca ccacgctacg caccccggtc gcctttggta acgaggagtg cgaccccgtc
     6901 tggctgcttc tctgcgtcag cgccacagat gccaacgcac atattttgac cattcagcgc
     6961 atcagccagt ttattgattc gccacaacgc ttagcggcag tgggaaatgc cagcacagac
     7021 gatgcactgt ttgcgctggt atcaggttaa cggaggcgct tatgatcctg cacccttctc
     7081 tcgccagcgc aaatcctctg cactatggtc gtgagctgac ggcattggac aatctggatt
     7141 ttgggagcct gcatctggat attgaggaca gcagcttcat taacaacatt acgtttggga
     7201 tgaaaacggt gcaggccgtc gccagacaga cgcctcatcc cttgtccttt catttcatgg
     7261 ttgcccggcc acagcgctgg tttaacgcgc tggctgaaat cagacccgcg tggatcttcg
     7321 ttcatgcaga aacgctggac tatccttcgg aaacgttaac tgagatccgc cataccggcg
     7381 cacgagccgg actggtattt aatccggcaa ccccgatcga cgcctggcga tatctggcgt
     7441 ctgagctgga cggagtgatg gtgatgacca gtgagcccga tgggcaggga cagcggttta
     7501 ttccctccat gtgcgagaaa atccaaaaag tacgcacggc ttttcctcag accgagtgct
     7561 gggccgacgg tggaattacg ctggcagctg cacagcaact ggcagcagcc ggggcgcaac
     7621 atatggtgat tgggcgcgcc ctgttctctt catctgacta tcgcgcgacc ttagctcaat
     7681 tcgccaccct gtaagaggag attgtttctg atgagccaac agagacctga ccgtatcaaa
     7741 cagatgctgc actacctgtg gcaacatcgt cacctctcca cacagcaggc catggagctt
     7801 tttggctatg cggaagcgac tgtccgtcgt gatttccagt atattgccaa ccagtatcca
     7861 ggcatgatcc gcggtcacgg ttgcctggac ttcgacgaca gtacagacga caaagagtac
     7921 gtcttcgacg tgaaacgtac attgcaatct gtggcgaaaa gagaaatagc tgcccttgcc
     7981 cgaacaatga tcaaagatgg cgactgcttt tttctcgact ccggctcaac ctgcctcgaa
     8041 ctggcgaaat gcctggccga tgcgcgggta aaggtcatct gcaacgacat taaaatcgcc
     8101 aatgaactgg gctgttttcc ccatgtggaa agttacatta tcggaggact gattcgccca
     8161 ggttattttt cggtaggaga gagtctggcg ctggagatga tcaatgcctt ttccgtagaa
     8221 cgtgcgttta tctcctgcga tgcgctgtcg ctggagacgg ggatcaccaa tgccaccatg
     8281 tttgaggtcg gggtgaaaac acggattatt caacgctcgc gtgaagttat tctcatggcc
     8341 gatcactcaa aatttgatgc cgttgagccg catgctgtag caacactatc atgtattaaa
     8401 acgatcatca gtgacagcgg gttacctgag actattgcac agcgttatca gcgagccgga
     8461 tgtcagttgt ttttacctca ttcaataaag tgataagtaa gttcgttcga gagggatttc
     8521 aagcaaaaat aatcaatggc acccaataga aaatattggc gatgcgctcg aacgaataaa
     8581 gaagccctat gcgcaatcca cacgctacga tgttgcaaca acacgccatc tactttttat
     8641 tctcattcac taaatgtggt tgttctggat tcattattca aagtgtgtac aagatcacat
     8701 ttaatcactt cattacagtt cagcatgctg aacaaagcat attttccact atgtaatgcc
     8761 gataccattt attccatgag caagaaggag ccattatggt tcgcaaaggg tgtaattcat
     8821 tagtccgtgc tgaaaaaata ttaacccaca ttgcatgggt tggtatggca a
//
LOCUS       DACADA010000173         8729 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.175, whole genome shotgun sequence.
ACCESSION   DACADA010000173 DACADA010000000
VERSION     DACADA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.175"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1080..1310
                     /locus_tag="ISB17_004068"
     CDS             1080..1310
                     /locus_tag="ISB17_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981942.1"
                     /translation="MQLLCDNNHSALTVQEFVMKKTFTFSLLAIVFASLLSACVPHHH
                     HRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
     gene            complement(1347..3275)
                     /gene="dhaR"
                     /locus_tag="ISB17_004069"
     CDS             complement(1347..3275)
                     /gene="dhaR"
                     /locus_tag="ISB17_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAP1981943.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSV
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRG"
     gene            3494..4564
                     /gene="dhaK"
                     /locus_tag="ISB17_004070"
     CDS             3494..4564
                     /gene="dhaK"
                     /locus_tag="ISB17_004070"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP1981944.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4575..5207
                     /gene="dhaL"
                     /locus_tag="ISB17_004071"
     CDS             4575..5207
                     /gene="dhaL"
                     /locus_tag="ISB17_004071"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAP1981945.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            5218..6636
                     /gene="dhaM"
                     /locus_tag="ISB17_004072"
     CDS             5218..6636
                     /gene="dhaM"
                     /locus_tag="ISB17_004072"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAP1981946.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6956..8653
                     /locus_tag="ISB17_004073"
     CDS             6956..8653
                     /locus_tag="ISB17_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAP1981947.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         2296 a         2206 c         2077 g         2150 t
ORIGIN      
        1 gttcaaattt gttacaacaa gcctgcagcc ttgccagata ggtaggttta aacataacag
       61 taaattccat tattgattat gttaatgcac atgcaggtac tcttacgcat ttaacggtga
      121 cgccaagatg acttgacggc agtcagaggg cgaatactcc cgaccttgtt aacactgatt
      181 gcgataccac aaccaacaaa tctacctgca tgtgccgtat taaattagca tagtcatcat
      241 ataactcaag cccccaatca atttttttca aaatcctcaa taagttatag atggtttgcg
      301 ttctgttcat ttctggcatc agagtgatga ttaggcaagt tagctgggaa aatttatccg
      361 gagtatatac aaagaaagta gtcagcccag ttgaatcatc taaacaagac caattaacta
      421 atttatttaa atgaaaaata atgcttttga gctaaccaat tatgcactta agctaaattt
      481 accgtttttg gggctagccc aacatcgagg gcgcataagc tttagcccac ttttactttc
      541 ctgttttctt tagtaacaga aggttaacta tcaaatattt ttgttgggct atgtgggcta
      601 acagttttca tatttacgtg tttttttatt cttctccggt ttccgtttca gataccggag
      661 taaacaacag cgtataagcc cagggacgtg catgcttgag gtgtctcagc cgtggcatac
      721 gtatcagcca tccctccacg ctggtggact ttttgagcat tccagcctca tgaaggattc
      781 tgccggcagt atccttgctg agaggagtag aacctacgcc cttcgcatta cgaatgcgag
      841 ctaactccaa aatgagtacg aacatgagta caaaaaaaat atatcattaa atgtagtata
      901 aatttcaatc agatacagca aaaattacaa ttcaacgtct aattccgcta atactactct
      961 ggcggttaat aacgagccat tatgtgagtt gcacgtaatg gcttttttac gcctgctacg
     1021 cgtcttcttt caattctgag ttaccaaaac tcataatcag actcctgaca atattaacaa
     1081 tgcagctttt atgtgataac aatcacagtg cattaaccgt tcaagagttt gtcatgaaaa
     1141 aaacatttac cttcagcctg ttagcaattg tctttgcaag cctgttgagt gcttgtgttc
     1201 cccaccatca tcaccgccac agcgatggcc ctcgtggtcc tgcgccctcc ggtaagccaa
     1261 tgccaccttc aaatggtgga ccaaatcacg gcagtcatgg tcgctattaa ttagtaattc
     1321 atagcgggaa agtacatact ctcccgctat ccacggcgct taaactgccc tgcatcaatg
     1381 ccatgttgct tcattttccg ccacagcgta gtgcggccga ttccaagtaa agccgacatt
     1441 tcctgaatgc gaccgcctgt gacttgggct gcgttaataa ttgcctcttt ttcaacttcc
     1501 gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc ctgctcggta
     1561 aacagatgtt ccggcaaatc actgacgcga atgcgcccat tatcactact cagagcaaga
     1621 ttctcgatga cgctgtaaag ttcaaaatcg ttgccaggcc atgcacagga aaccaggcga
     1681 gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag gctgcgtaat
     1741 ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagtggcgg gatggtaatt
     1801 tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc caccagcatt
     1861 gcgaggtccg cggtcgttgt ggcaatcact ttgacatcaa tgggtattaa acgccgcgca
     1921 tccagtcgcg tgataacacc ctgcttgata acctgaagca aagcagactg taactccacc
     1981 gccagatatt caatcttttc aagaaacagc gtgccgccgt gcgccagttc cagccgactc
     2041 agacggccat tttcattatc cgtgcgatcg ccaccaataa attcttccgc cagcgccgca
     2101 tcaccatata actcacaatt gacggcgata taaggccccg ctgcacgctc gctttcatta
     2161 tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag caggacagga
     2221 aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg cggatcgtcc
     2281 tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat taactgccgc
     2341 atctgttcca caggatggag caataaaata aagctggttc cctgcgtttc tattatcggt
     2401 ttaagggtta tcaccgcatc aataaactga tgctgacttt caaaggttgc ttctacgtgt
     2461 ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg cgggtaacgt taagagttca
     2521 gtgattgccc ttccctgact tgccgtcgcg tcaaggcgca agactcgcgc cgcctgggcg
     2581 ttgataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc catactttct
     2641 aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact gtccgtcagc
     2701 agtaaatttc cgacctcgcg tgcgattgcc aacgtcaacg gcaaatcagc tgcggtagtt
     2761 tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct gtcaaacaac
     2821 ggcgttgcgc aaaaggccca gttccagagt gcctgtttga aatgttgatc ggccatcgtt
     2881 ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat aattccctcg
     2941 gcgcaatacg tgccgtcatt gaatcccagt acacttagct gctgcaaggt ttgcggatcg
     3001 ccattacggc tgagaatgca ggcggtttca tcgaggataa gcaacgcaca ctctcgcggt
     3061 gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat cgctttctta
     3121 cgccgataaa tagaagcaaa tgtcacgccc tgggcctggt gtggtacgtt ccatgtctcc
     3181 cgtttcatca gcttattgca tctctcccag gaggttgcaa ttaagggaga tatgccccga
     3241 ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag gtgttccggc
     3301 tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacactca ttaaagcata
     3361 tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga tgcacaatgg
     3421 attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct accgtaattg
     3481 ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg acgaacaact
     3541 ggcaggacta gcgaaagcgc atccatcgct gacactgcat caggatccgg tgtatgtcac
     3601 ccgagctgat gcccccgttg caggaaaagt cgccctgctg tcgggtggcg gcagcggaca
     3661 cgagccgatg cattgtggtt atatcggtca ggggatgctt tcgggggcct gtccgggcga
     3721 aattttcacc tcaccgacgc ccgataaaat ctttgaatgc gccatgcaaa ttgatggcgg
     3781 cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact tcgaaacagc
     3841 aaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg atgacgacgt
     3901 tgcagtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca ccgtattaat
     3961 tgaaaaactc gtaggtgcag cggcggagcg tggcgactca ctggacgcct gtgcggaact
     4021 ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg cctgtaccgt
     4081 tcctgccgcg ggcaaacctt cttttaccct ggcggataat gagatggagt ttggcgtcgg
     4141 cattcatggt gagccaggta ttgaccgccg ccccttctct tcccttgatc aaaccgtcga
     4201 tgaaatgttc gacaccctgc tggaaaatgg ctcataccat cgcactttgc gtttatggga
     4261 ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc agtctggcga
     4321 tcgggtgatt gcgctggtta acaatcttgg cgcaactccg ctttctgagc tgtacggcgt
     4381 ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac gtaatttaat
     4441 tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac tgaaagttga
     4501 tgacgaaacg ctggcactct gggacgcccc ggtccacacc ccggccctta actggggtaa
     4561 ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc actcgttgtg
     4621 gcgatatttt cagcaccgag agcgagtatc ttactggact ggatcgcgag attggcgacg
     4681 ctgaccacgg gctaaatatg aaccgaggct ttggcaaagt ggtggaaaaa ctccctgcta
     4741 tcgcagataa agatatcggt ttcattctca agaataccgg catgacgcta ctttccagcg
     4801 tcggaggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca cagacaaccc
     4861 aggcacggca aagcctgacg ctggaagagc tttatcagat gttccgcgat ggcgcggacg
     4921 gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat gtgtgggtgc
     4981 cggtggtgga atcgttacgt cagtcctgcg agcaaaatct ctctgttccg gcggcgctcg
     5041 acgctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg caagcccgca
     5101 aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc ggcgcgacct
     5161 cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga attggtgatg
     5221 gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg tgaattagcc
     5281 cgtcagatgt taatgagtga taattgtaaa atcgccattg ccgcgggaat tgacgatcca
     5341 caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc tgttgccgat
     5401 gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc tgaaactgcg
     5461 ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc gccgttggtc
     5521 gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga caaagttatc
     5581 tttgacgcca tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc gtcctccgac
     5641 actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct ggcggtggtc
     5701 ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta taccttatcg
     5761 acatttaatg ccgatatgtt gctggagaaa aacggcaaat gcgttacacc ggagagtatt
     5821 aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat tgcgaaagga
     5881 ccagaagctg aagaggcact gatcgctttc cgtcagttgg ctgaagataa ctttggtgaa
     5941 acggaggaag ttgctccacc tactctgcgt cccgttccgc ctgtttcggg taaagccttt
     6001 tattatcaac ctgttttatg tacggtacag gcaaaatcaa ccctgaccgt ggaagaagaa
     6061 caagaacgat tacgccaggc cattgacttc acgttattag atctgatgac gttaacagcg
     6121 aaagcagaaa ccagcgggct tgacgatatt gccgcaatct tttctggtca ccatacactg
     6181 ttagatgatc cggaactgca ggcggcggca agcgaactcc ttcagcatga acattgcacg
     6241 gcggaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca acagctggat
     6301 gatgaatatc tacaggctcg ctatatcgat gtggacgatc ttctgcatcg caccctggtc
     6361 cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat tctactggcg
     6421 gagaacattt atccctccac agtactgcaa ctggatccag cggttgtaaa aggtatctgc
     6481 cttagcgccg gaactccgct atcccacagt gccctaatcg cccgtgaact gggaattggc
     6541 tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac gctaacgctg
     6601 gacgttaaaa cgcaacgttt caaccgtcag ggttaaacca ccgttcggat ggcatcattc
     6661 tgatgtcatc cggcaattta ctctgcactg atgtttttta actacctgtt atatttttca
     6721 ccaaattatg atgatttttc ttcgctaatc gcgaacccaa cgtcgctggc tgacgaataa
     6781 acagatgtta ttttatcact ctatttcgcc gttatttatc cctttaattc ccttttctaa
     6841 aatgtctgtc agttcgcaga atgtgatttc gatcatgcag ctagtgcgat cctgaactaa
     6901 ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat gattgatgaa
     6961 atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgca tctttttgtg
     7021 tttcgctgcg ctatcggtgc aggcagaaga aacatcggta acaccacaac cgcctgatat
     7081 tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg accaaaaaac
     7141 ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt atcggatgca
     7201 gcaaaaccag agcggatttg atctacgcca tttcgttaac gtcaatttca ccctgccgaa
     7261 agagggcgag aaatatgttc cgccagaggg gcaatcactg cgcgaacata ttgacggact
     7321 ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc tattaccgct
     7381 gccaaagcct tatgtcgtgc caggcggacg ttttcgcgag gtatattact gggacagtta
     7441 cttcaccatg ttaggacttg ccgaaagcgg tcactgggat aaagtcgcgg atatggtggc
     7501 caattttgct catgaaatag acacttacgg tcatattccc aacggcaacc gcagttacta
     7561 tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg cgcagcatga
     7621 aggcgacgcc gcgttgaaac aatacctgcc gcaaatgcaa aaagaatatg cttactggat
     7681 ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa aacttcagga
     7741 tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag agtcatgggt
     7801 ggaagatatt gccactgcca aaagcaatcc gaatcgacct gccactgaaa tttaccgcga
     7861 cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg acaatccgca
     7921 gcagttaaac accttacgca ccaccagcat cgtaccggtc gatctgaaca gcttgatgtt
     7981 caaaatggaa aaaatcctcg cccgcgccag caaagctatc ggagataacg cgatggcaaa
     8041 ccagtacgaa acgctggcaa atgcccgtca aaaagggatc gaaaaatacc tgtggaacga
     8101 tcaacaaagc tggtatgccg attacgacct gaaaagtcat aaagtgcgca atcagttaac
     8161 cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg ccagcaaaat
     8221 ggcgacggcg acgaaaacac atctgctgca accaggcggc ctgaacacca cgtcggtgaa
     8281 aagtgggcaa caatgggatg cgccaaacgg ctgggcgccg ttgcagtggg tcgcgacaga
     8341 aggattacaa aactacgggc aaaaagaggt agcgatggac attagctggc acttcctgac
     8401 caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg atgtcagcac
     8461 caccggaaca gggggcggcg gtggcgaata tccattacag gatggctttg gctggaccaa
     8521 tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt gtgacaatgt
     8581 tccggcgacg cgtccactca gtgaatcaac aacacagccg ctaaaacaaa aagaggcgga
     8641 acctacgcct taactttact gtatatcgag caatgccatt acgtgcataa aattggcatt
     8701 gctctttctt catcacgaaa taccactct
//
LOCUS       DACADA010000174         8710 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.176, whole genome shotgun sequence.
ACCESSION   DACADA010000174 DACADA010000000
VERSION     DACADA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.176"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            53..1102
                     /gene="allD"
                     /locus_tag="ISB17_004074"
     CDS             53..1102
                     /gene="allD"
                     /locus_tag="ISB17_004074"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAP1981948.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1124..2359
                     /gene="allC"
                     /locus_tag="ISB17_004075"
     CDS             1124..2359
                     /gene="allC"
                     /locus_tag="ISB17_004075"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAP1981949.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2370..3155
                     /gene="allE"
                     /locus_tag="ISB17_004076"
     CDS             2370..3155
                     /gene="allE"
                     /locus_tag="ISB17_004076"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAP1981950.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVYGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3284..4429)
                     /gene="glxK"
                     /locus_tag="ISB17_004077"
     CDS             complement(3284..4429)
                     /gene="glxK"
                     /locus_tag="ISB17_004077"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAP1981951.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4451..5752)
                     /locus_tag="ISB17_004078"
     CDS             complement(4451..5752)
                     /locus_tag="ISB17_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAP1981952.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5808..7169)
                     /gene="allB"
                     /locus_tag="ISB17_004079"
     CDS             complement(5808..7169)
                     /gene="allB"
                     /locus_tag="ISB17_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAP1981953.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7229..8683)
                     /locus_tag="ISB17_004080"
     CDS             complement(7229..8683)
                     /locus_tag="ISB17_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HAP1981954.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2336 a         2298 c         2092 g         1984 t
ORIGIN      
        1 cctgtatgtt aattgcagtc atgcttcaca ccgtcgttaa aaaggaagac agatgaaaat
       61 cagtcgggaa acactccacc agctaatcga gaataaactc tgccaggctg ggttaaaacg
      121 tgagcacgct gcaaccgtgg ctgaagtatt ggtttacgcc gatgccagag ggatccactc
      181 tcatggcgcg gtgcgcgttg aatactacgc cgaacgcatt tcaaaaggcg gcaccaaccg
      241 cgaaccggag tttcgtcttg aggaaaccgg accgtgctcg gcaattttac atgccgacaa
      301 tgccgccgga caggtcgcgg cgaaaatggg tatggaacat gccatcaaaa ccgcccagca
      361 aaaaggcgtt gcggtggtcg gtatcagccg gatgggtcac agcggcgcaa tctcttattt
      421 tgtgcagcag gctgcccgcg ccggattgat tggcatttcg ctgtgccagt ccgatccaat
      481 ggtggtgccg tttggcggcg cggaaattta ctacggcact aacccactgg cctttgccgc
      541 gccgggagaa ggcgacgaga tccttacctt tgatatggcg actaccgtac aggcatgggg
      601 aaaagtactc gacgcccgct cgcgcaatat gtctatcccg gatacctggg cagtcgataa
      661 aaacggtgca ccaacaaccg atccgttcgc ggtacatgct ctgctccccg ccgctgggcc
      721 aaaagggtat ggcctgatga tgatgattga cgtcctctca ggcgtcttac tcggcttacc
      781 gttcgggcga caggttagtt cgatgtatga cgatttacac gccgggcgta atttggggca
      841 attacatata gttattaacc cgaacttttt ctcctccagc gaattattcc gtcaacatct
      901 tagccagacc atgcgcgaat taaataccat tacccccgcg cccggtttta atcaggttta
      961 ttatcccgga caggatcagg atattaaaca acgcaaagcc gccgtcgaag gcatcgaaat
     1021 tgttgatgat atttaccagt atttaatttc cgacgcgctt tataacacgt catacgaaac
     1081 gaaaaatccc tttgcgcaat aattattgag acaggacttt cttatgatta cacatttccg
     1141 tcaagctata gaagaaacgc tgccctggct ttcctctttt ggcgctgacc cagcgggtgg
     1201 gatgacccgt ttactttatt cgccggaatg gctggaaacc cagcagcagt ttaaaaaaag
     1261 aatggcagca agcgggctgg aaacacgttt cgatgaagtg gggaatttat acggtcgcct
     1321 gaatggcacc gaatatccac aggaagtggt tctgagcggt tcgcatatcg ataccgtggt
     1381 taacggcggt aacctcgacg ggcaattcgg tgcgctggcg gcgtggctgg caattgactg
     1441 gctgaaaacg caatacggcg cgccgttacg tacggtcgaa gtggtggcga tggcagaaga
     1501 agaaggcagc cgcttcccgt atgttttctg gggcagtaaa aatatttttg ggctggcgaa
     1561 tcctgacgac gtgcggaata tctgtgatgc caaaggaaat agttttgtcg atgcgatgaa
     1621 ggcttgcgga tttactctgc cgaacgcccc actaactccg cgtcaggata ttaaagcctt
     1681 tgtcgaactg catatcgaac agggctgtgt gctggaaagt aatgggcagt caattggcgt
     1741 ggtgaatgca attgtcgggc aacgtcgcta tacggtgacg ctgaacggtg aatcaaacca
     1801 tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt tacgctttca gtcgcatttg
     1861 ccatcagtct gtcgaaaaag cgaaaaagat gggcgacccg ctggttctga cttttggcaa
     1921 agtagagccg cgcccgaata cggtgaatgt agtgccgggt aaaaccacgt tcaccattga
     1981 ttgtcgtcat accgatgcta ccgtgctgcg cgatttcacc caacagttag aaaacgacat
     2041 gcgggcgatt tgcgatgaaa tggatattgg tattgatatc gatttatgga tggacgaaga
     2101 acccgtgccg atgaataagg agctggtcgc caccctgaca gaattgtgtg aaagcgaaaa
     2161 actgaattac cgggtgatgc acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg
     2221 cgtgccgacc tgcatgattt tcattcccag catcaacggg atcagccata acccggcgga
     2281 acgcaccaat attaccgacc ttgccgaagg ggtcaaaacg ttggcactca tgctttatca
     2341 acttgcctgg cagaaataag gagttataaa tgggatattt aaataacgtc accggttacc
     2401 gcgatgattt actggctaac cgtgcgattg ttaaacacgg taatttcgca ctgttaacac
     2461 cagacggtct ggtaaaaaat attattccgg gctttgtaaa ttgtgacgcg acaatcctct
     2521 ccacgccaaa gctgggtgcc tcttttgttg attatctggt cacactgcat caaaacggcg
     2581 gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa
     2641 atatcactgc caaagccgaa gggaaaacat ttgccttaag cgaaggtggc tatctttatt
     2701 gcccgccagg ctccttaatg acgtttgtta acgcccaggc cgaagacagc caaatctttt
     2761 tatataagcg tcgctatatt ccggtagaag gtcatgcgcc gtggctggtt tatggcaatg
     2821 ccagcgaact ggaacgcatt cattatgaag gcatggacga tgttattctg ctggattttc
     2881 tgcccaaaga gttaggtttt gatatgaaca tgcatatcct ctcttttgca ccaggtgcca
     2941 gccacggtta tatcgaaacc cacgttcagg aacacggtgc ctatattctt tccggtcagg
     3001 gggtttataa cctcgacaat aactggatcc cggtgaaaaa aggcgattac atctttatgg
     3061 gcgcttattc tttacaggct ggttatggcg tagggcgtgg tgaagcgttc agctatattt
     3121 actcgaaaga ttgcaaccgc gacgtagaga tttaagtcat accactcgcc tgattatttt
     3181 tagctatgtt ggattttgcc acggtttatt ccgtggcttt ttttgtagtc aattcaggta
     3241 aaaaaagatg cgatataaat cgcatccgct ttaaaggtaa tagtcagttt ttaattccct
     3301 gacctatttt aatggcgcag gcaatatttc gcgcactgtt gaagagattg gtttcaccgc
     3361 tggcgagcac ttcggctaaa ggagccagac gtggcaaaat gctgaatacc gcatcaatgc
     3421 cgtactggtg caccacttcc acgccatcac ccaatacgcc agcaatccca atcaccggga
     3481 cattaaactg cttcgccacc gatgccacac ccagcggcgc tttaccgcct gccgtttgcg
     3541 agtcgatgcg cccttccccg gtgatcacca gtgctgcacc ctgcactgcc tgcgcaagat
     3601 tgaccgcatt caacacaatc tcaatacccg gtttaatatc cgcattgagg aataccgccg
     3661 ccgcaatgcc cataccgccc gccgcgccac cacccgccat ctggcagacg ttgatttcag
     3721 ttagctgttg taaaacacgg gcgtaatttt gcagcccctg ttccagctct tcgaccatct
     3781 ccggcgttgc ccctttttgc gggccaaaca ccgccgccgc gccgcgtgcc cctaccagcg
     3841 ggttatcaac gtcacatgct acttcaatat gacagttcgc cagacgcgga tcgcattcat
     3901 tcatctcaat acttgccacg cgcgctaaat tgccgccgtt agccgccagc ggctgaccgt
     3961 cggcatcaag gaaacgcaca ccgagcgcct gcgccatgcc cataccgccg tcgaccgtcg
     4021 cactgccgcc aatgccgaga ataatatggc gaatgccatt atccagcgca tggagaatta
     4081 actcccccgt accaaaactg gaggccagca acggattacg cttttcaggc acgaccagca
     4141 tcaggccact tgctgctgcc atctcaatca ccgccgtttt cccgtcgccg gtaatgccat
     4201 aaaaagcatt cactttttcg cccatcggcc cgcagacttc aagcttcacg atgttaccgt
     4261 ccgtcgcggc gaccatcgcc tccaccgtcc cttcgccgcc atccgctatc ggcaaacaga
     4321 tatagtgcgc atcgggaaat atggtcgaaa acccggcttt aattgcctga caacattttt
     4381 ctgcacttaa gctctcttta aaagagtctg gcgcaatgac aatcttcatt atcaatcctt
     4441 tttcaacaaa ttattcgatg cgttcccacg gaataagatt atccataaga acagccagta
     4501 aaattccgac cagtaagccg ttactgagca gaggacgaag cgttaatgga aggtcttgca
     4561 gatacacagg cggtaatgcc attaaaaata tgccgacaaa taacggcaat gcgagtcgat
     4621 aaatattgcg agcggtaaac gttatttgct ggctaaacac cagcgcggaa aagagtaaag
     4681 gcagataaga aaccagcatg accgcactac tcactggtaa agggatactg caaaacagtc
     4741 gcgtgagtgc cggaaccagt gccaccagca ggcaaataac gctgccataa ataaacgaac
     4801 gccgcgagta atcgcctgtc tgggttaata aaccaatgga tgaaacaaac ggtgaaaatg
     4861 gaattactgc cagcggcacg gttatcagtg tcataaatcc ggtcgccaca aagctgcgac
     4921 gataacgcgt attccccgcg ccctgctgcg gataaaaaac atccgtgccc cgaatcgcac
     4981 cgtaggtatt gctgatattt accagacctg taatcaccgc cgtcagaata atccccggcg
     5041 acaaagcacc gccactgccg agcgggaacc actgccagtg caactcaccg gagagcgaat
     5101 gcgaagaagg aaagcaaaag taccacaaca accagccagt tatcgtgccg accagcaggc
     5161 cgtaacgggc aaaacgttgt ggcaggaaaa taatcatcgc cagtaccagg cacatcaccg
     5221 ccaccgagag cgcgaacggc ggtaattgaa ttttaaaatt cggggcggct atgccaaacg
     5281 gcagcccgag catacctttg aaaaagatag tggtcagttg cgcacccagc atcaacataa
     5341 acaagaccat caccgacggc gtaaataacc gtgccaggcg atggcctaat ccgctaaaac
     5401 caatcaacat cgtcagcacg ccggagagcg caatccccac cgccagactg gtagcgatat
     5461 cgttgatcgg tgtcccgcgc gatgcttcgc caagggtgat agtcaggatg gttccccacc
     5521 acaggccgcc cggcccttcc ataatagcgc gacgatgacc gcagaacgcc tgagcgaagc
     5581 aggccagtgc ggtagcaaga aaagcatatt gcgtgagcgt aagcaggcta ctttgcggca
     5641 actgaaaagc agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa ataaagaaaa
     5701 accactggaa tcctgataac aggctttcgc ggcccactgc aaaattgaac ataaacacac
     5761 cttaaccgga gaatgcccgc cacaaggacg ggcattgcag ggccagatta ctgctgatgt
     5821 ttaaggataa attgaccttt cggcgcaaca gggaagccct gttcgatgtc gtaaatcaca
     5881 tcaccacgta agatggtttt cgtgatacgc gcgccaatag tacggccaac atacgggctg
     5941 actttgtggc gatattccag atcgtcattg gtaagaacat agctgctatt cggctgaatg
     6001 aagacgaagt cggcatcttt tcctggggcg atacggcctt tttgctgcag accgaaaata
     6061 tctgctgcgt tagtcgccat taatttgccg aacattggca gagacattcc gcgtttctgt
     6121 accgcttcat cgaacatcac gtccatgcag ctttgcagac cagcgatacc gccccacgct
     6181 ttcatgatgt taccggcttt catttccggc gggcatggag agtggtcgga aaccaggcag
     6241 tctatttcac cgttaaacag tttttcccac atgcctttct ggttttccag atcgcggatc
     6301 ggcggtgaac acttcgccag agtaccgatt tcttcgaact gatcggtatc cagcacaaag
     6361 taatgcgggc aggattcaca agtaacatcc tgaccttcct gacgtgcacg agtcacttcc
     6421 tcaacacctt ccgggctgct gacgtggcaa acgtgcagac ggcaaccagc gactttcgcc
     6481 aggtacagta cgcggcgaat cgcttccact tcggtaaata ccggacgcga agccacatag
     6541 tcatgggcag ttacgcgacc ttcacgcttc gcttcttcgc ccagtgcgtc acagatcagc
     6601 gcgttttcgc agtgcaccag cactggctgg cccagttcgc ccagcttctg cgcgcctttg
     6661 aagaattgcc agtcattgac gtcacggaag tcgttgtcga taccgcgatc gccacaggtc
     6721 gcaacgaagc atttgaaacc gacaacaccc acttcatcca actcatgcag acggtcaatg
     6781 ttgtaagaca ccaggccacc gagttgtgcc gcatcgatag tcagctttcc tttagcggca
     6841 tcgaacttca gttcaattga ggcgcggtca accgttgcag gcagctggtt gagcggcatt
     6901 tcgatcatgg tggtgatacc accttttgct gctgcgcgag taccggtttc ataaccttcc
     6961 cagtggctgc gacccggttc agaaatatgg gtgtgcgcat caaccatgcc cggcgaaacc
     7021 accagaccag acgcatccat aacgtctttt gcatcgccca gatcctggcc gatagcagca
     7081 atttttccgt ctttaacggc gatatctaca acgcgagctt cgttttctaa aataacggtg
     7141 ccgtttttaa tgattaaatc aaaagacata acaaactcct tgtaaaatac agattggttt
     7201 ttaactcaat gttcaatatc gtgattaatt aacctatggt tttttgttct cctgtttttt
     7261 ctgctgttgt acgtttcttt aataaggcgt aggccgcaaa ggcgacgatg acgccgacaa
     7321 accatgaaac acgtgataac ggttccataa agggaataaa cttaccgcca agagataaaa
     7381 taacggccac cagagttact gaaaacgcgg tgaggttaaa accgttatcg taatatttat
     7441 aatcgccagg tgcggtgtac agttcatcaa gattaatttg cccacgcatc accacaaaat
     7501 aatgcgccat catcacacca attaccggac caagcattcc gccgataata tcgaggaaca
     7561 gataaatact gtcctgattt tccattaatt tccacggaca gatcagcagg ctgataatac
     7621 tggcaatcag tacgccgttt ttataggtca gtttcgtcgg tgcaatggcg gcaatctgat
     7681 aaccagccgg aataatatta ccggtggcgt tagtagagat agtcgtcatc agaataacca
     7741 gtaccgcaaa gaacgaggcg aacaggctgt cccaacgctg aacaatatcc agcacgttcc
     7801 aggtgtcagc gccgtaatga atactggctc cggcaataat acataccccg gcgaccgcaa
     7861 acagaatata ggccacaact aaacccagcg tttgccccag cgcctgctca cgaaacgagt
     7921 gggcgttttg cgtaaagtcg gatgcgctca ccgccggtgc cgcccagacc gcaaccaccg
     7981 cgtttatcac caccagaaac aggaagccac tgttttctgc tttctgaata ccgcccggaa
     8041 tgtagtcaaa gattggaccg atcccgacca gcgaaatcgc ccaaatcgcc ataccgccga
     8101 aaacgatata gatgcacggg ttaagaatgg cagtgaattt atttaaaact tttccgccgc
     8161 caaaaccaat accaacgtta accagccaga agagtaagaa agtaattaat cccggtagag
     8221 aaaggcctaa cagcgtgaaa tcgccaccga gagtcaaaaa tcccggccag attttgccaa
     8281 tcagaatcaa gcaggccagt gaccccgcgt aacattgcag gccaaaccac ataatggcag
     8341 caataccgcc tcttaataat ccgggaaaca gtgcaccacg tacgccgtaa gaagcacgca
     8401 ggatcatggc aaacggtacg ccgtatttac tgcccgcagc accgtttaat accattaccg
     8461 cggcaatgaa aaaggcactg aggataattg ccagcataat actaaaggta gacaagccga
     8521 gaataaaaaa gccgccaacc atcacataat tgggaacgtt atgaaccgaa cccatccata
     8581 aggtaaaata gttaaatgtt ttccaggtcc gctgactttg cgttttcggc agtagatctt
     8641 cgctatagcc gcgttgctgg aatagttttc tctgatgttc cataaatccc tcttaatttc
     8701 acgtatcgaa
//
LOCUS       DACADA010000175         8668 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.177, whole genome shotgun sequence.
ACCESSION   DACADA010000175 DACADA010000000
VERSION     DACADA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.177"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            80..601
                     /locus_tag="ISB17_004081"
     CDS             80..601
                     /locus_tag="ISB17_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP1981955.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            598..1551
                     /gene="fecR"
                     /locus_tag="ISB17_004082"
     CDS             598..1551
                     /gene="fecR"
                     /locus_tag="ISB17_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAP1981956.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRILTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1638..3962
                     /gene="fecA"
                     /locus_tag="ISB17_004083"
     CDS             1638..3962
                     /gene="fecA"
                     /locus_tag="ISB17_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAP1981957.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4007..4909
                     /gene="fecB"
                     /locus_tag="ISB17_004084"
     CDS             4007..4909
                     /gene="fecB"
                     /locus_tag="ISB17_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAP1981958.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4906..5904
                     /gene="fecC"
                     /locus_tag="ISB17_004085"
     CDS             4906..5904
                     /gene="fecC"
                     /locus_tag="ISB17_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAP1981959.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5901..6857
                     /gene="fecD"
                     /locus_tag="ISB17_004086"
     CDS             5901..6857
                     /gene="fecD"
                     /locus_tag="ISB17_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAP1981960.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6858..7625
                     /gene="fecE"
                     /locus_tag="ISB17_004087"
     CDS             6858..7625
                     /gene="fecE"
                     /locus_tag="ISB17_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAP1981961.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8182..8439)
                     /locus_tag="ISB17_004088"
     CDS             complement(8182..8439)
                     /locus_tag="ISB17_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981962.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8522..>8668)
                     /locus_tag="ISB17_004089"
     CDS             complement(8522..>8668)
                     /locus_tag="ISB17_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP1981963.1"
                     /translation="FSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAE
                     ASFS"
BASE COUNT         1864 a         2476 c         2426 g         1902 t
ORIGIN      
        1 caattttatt tccaattgta atgataacca ttctcatatt aatatgacta cgtgataatt
       61 aacttttgat gcactccgca tgtctgaccg cgccactacc acagcttcct taacgttcga
      121 gtcgctttat ggcacacatc acggctggtt gaaaagctgg ctgacgcgca aactccagtc
      181 tgcttttgat gcagatgaca ttgcccagga cacttttttg cgggtaatgg tcagcgaaac
      241 gctctcgacg atccgcgatc ctcgctcctt cctctgcact atcgccaaac gcgtgatggt
      301 ggacctgttt cgccgaaacg cgctggaaaa agcgtatctg gagatgctgg cgcttatgcc
      361 ggagggggga gcgccttcac ctgaggaacg cgaaagccaa ctcgagaccc tacaactcct
      421 cgacagcatg ctggacgggc taaacggcaa aacacgtgaa gcgtttctgc tttcgcaact
      481 ggatggtctg acatacagcg agattgcgca caaactcggt gtttccatca gctccgtgaa
      541 aaaatacgtg gcgaaagccg tcgagcactg cctgctgttc cgtctggagt atgggttatg
      601 aatcctttgt taaccgattc ccgccgtcag gcgctgcgtt cagcttccca ctggtatgcc
      661 gtgctaagcg gcgagcgcgt cagcccacaa caggaagcgc gctggcaaca gtggtatgaa
      721 caggatcagg ataaccagtg ggcctggcag caggttgaaa acctgcgcaa ccagcttggc
      781 ggtgtgcctg gcgacgttgc cagccgggcg ttgcacgata cccgcctcac ccgccgtcac
      841 gtgatgaaag gattactgct gttgctcggc gctggcggag gctggcagct ctggcagtcg
      901 gaaaccggcg aaggtctgcg ggcagattac cgcaccgcca aaggcacagt cagccgtcag
      961 caactggaag atggctccct gctcacgctg aatacccaaa gcgcggcgga tgtgcgtttt
     1021 gatgcgcatc agcgcaccgt ccggctctgg tacggtgaaa tcgccattac taccgcgaaa
     1081 gatgccctgc aacgcccctt ccgtatcctg acccgtcagg gccagctcac tgctttaggg
     1141 acagaattta ccgtccgcca gcaggataat ttcacgcagc ttgacgttca gcagcacgct
     1201 gtggaagtgc ttctcgccag tgcccccgcg caaaaacgca tcgtgaacgc tggtgaaagc
     1261 ctgcagttca gcgcctctga gtttggcgca gtgaaaccgc tggatgacga gagtacaagc
     1321 tggacgaagg acatcctgag cttcagcgat aaaccactgg gtgaggtgat agccacgcta
     1381 acccgttacc gcaacggcgt gctgcgctgc gatcccgccg ttgccgggct gcgcctgagc
     1441 gggacgttcc cgctgaaaaa taccgatgcg atcctgaacg ttatcgcgca aacgcttccc
     1501 gttaaaattc agtctattac gcggtactgg ataaacattt caccactgta aggaaaataa
     1561 ttcttatttc gattgtcctt tttacccttc tcgttcgact catagctgaa cacaacaaaa
     1621 atgatgatgg ggaaggtatg acgccgttac gcgtttttcg taaaacaaca cctttggtta
     1681 acgccattcg cctgagcctg ctgccgctgg ccggtctctc gttttccgct tttgctgcac
     1741 aggttaatat cgcaccggga tcgctcgata aagcgctcaa tcagtatgcc gcacacagcg
     1801 gatttaccct ctcggttgac gccagcctga cgcgcggcaa gcagagcaac ggcctgcacg
     1861 gcgattacga cgtcgagagc ggcctgcaac aactgctgga cggcagcgga ctgcaggtaa
     1921 aaccgctggg aaataatagc tggacgctgg agcccgcgcc cgcaccaaaa gaagatgccc
     1981 tgaccgtggt cggcgactgg ctgggtgatg cgcgtgaaaa cgacgtattt gaacatgctg
     2041 gcgcgcgtga cgtgatccgc cgtgaggatt tcgccaaaac cggcgcaacc accatgcgtg
     2101 aggtacttaa ccgcatccct ggcgtcagcg cgccggaaaa caacggcacc ggcagccacg
     2161 acctggcgat gaactttggc atccggggcc tgaacccgcg cctcaccagc cgctcgaccg
     2221 tcctgatgga cggcatcccc gtccccttcg ccccttacgg tcagccgcag ctttcactgg
     2281 ctcccgtttc gctcggcaac atggatgcca ttgacgtggt acgcggtggt ggtgcggtgc
     2341 gttacggacc gcagagcgtg ggcggcgtgg tgaactttgt tacccgtgcc attccgcagg
     2401 actttggtat cgaggcgggc gtggaaggtc agctcagccc aacctcttca caaaacaacc
     2461 cgaaagagac gcacaacctg atggtgggcg gcacagcgga caacggtttt ggcaccgcgc
     2521 tgctctactc cggcacgcgc ggcagtgact ggcgcgagca cagcgccacc cgcatcgacg
     2581 acctgatgct gaaaagcaaa tatgcgccgg atgaggtgca caccttcaac agcctgctgc
     2641 aatattacga cggtgaagcc gacatgcccg gtggcctgtc tcgcgcggat tacgacgccg
     2701 atcgctggca atccacccgc ccgtatgacc gcttctgggg tcgtcgcaag ctggcgagcc
     2761 tgggctacca gttccagcca gacagccagc ataaattcaa cattcagggg ttctacaccc
     2821 aaaccctgcg cagcggctac ctggagcaag gcaaacgcat caccctctcg ccgcgtaact
     2881 actgggtgcg cggtattgag ccacgctaca gccagatctt tatgatcggc ccttccgcgc
     2941 acgaagtggg cgtgggctat cgctatgtga atgaatcaac gcatgaaatg cgttactaca
     3001 ccgccaccag cagcgggcag ttgccgtccg gctcaagccc ttacgaccgc gatacgcgtt
     3061 ccggcaccga ggcgcacgcc tggtatctgg atgacaaaat cgacatcggc aactggacca
     3121 tcacgccggg tatgcgtttc gaacatatcg agtcatacca gaacaacgcc atcacaggca
     3181 cgcacgaaga agtgagctat aacgcaccgc ttccggcgtt gaacgtgctc tatcacctga
     3241 ctgacagctg gaatctttat gcaaacactg aaggctcgtt cggcaccgta cagtacagcc
     3301 agattggcaa ggctgtgcaa agcggcaatg tggaaccgga aaaagcgcga acctgggaac
     3361 tcggtacccg ctacgacgac ggcgcgctga cggcggaaat ggggctgttc ctgattaact
     3421 ttaacaatca gtacgactcc aaccagacca acgacaccgt cactgcacgt ggcaaaacgc
     3481 gccataccgg gctggaaacg caggcacgtt acgatctggg tacgctaacg ccaacgcttg
     3541 ataacgtttc catctacgcc agctatgcgt atgtgaacgc ggaaatccgc gagaaaggcg
     3601 acacctacgg caatctggta ccattctccc cgaaacataa aggcacgctg ggcgtggact
     3661 acaagccagg aaactggacg ttcaatctga acagcgattt ccagtccagc cagtttgcgg
     3721 ataacgccaa tacggtgaaa gagagcgccg acggcagtac cggccgcatt cccggcttca
     3781 tgctctgggg cgcacgcgtg gcgtatgact ttggcccgca gatggcagat ctgaacctgg
     3841 cattcggtgt gaaaaacatc ttcgaccagg actacttcat ccgctcttat gacgacaaca
     3901 acaaaggcat ctatgcaggc cagccgcgca cgctgtatat gcaggggtcg ttgaagttct
     3961 gatgtgttaa cgcccggctt gccgggcttt tagctggaat gtgattatgt tggcatttat
     4021 ccgttttgtt tttgcaggcc tgctgctggt gatcagccac gcctttgccg ccacggttca
     4081 ggacgaacac ggcacgttta cactcgaaaa aacgccacaa cggattgtgg tgctggaact
     4141 ctcgttcgcc gatgcgctgg ccgccgtgga cgtcagcccg atcggtattg ccgacgataa
     4201 cgatgcaaaa cgcatcctgc ccgaagtgcg tgcgcacctg aaaccgtggc agtccgtcgg
     4261 aacgcgcgcg cagccgagcc tggaagccat tgccgctctg aaaccagacc tgatcattgc
     4321 cgacagcagt cgccatgcgg gggtttacat cgccttgcag caaatcgcgc cggtactgct
     4381 gcttaagtcc cgcaacgaaa cctacgctga aaatttgcaa tctgcggcta tcatcggcga
     4441 aatggtgggt aaaaagcgag agatgcaggc acgcctggaa caacataaag agaggatggc
     4501 gcagtgggcc agccagcttc ccaaagggac acgcgtggcc tttggcacat cacgcgaaca
     4561 gcaattcaac ctgcatactc aggagacctg gaccggcagc gtgctggcct ctctggggct
     4621 gaacgttccc gctgcgatgg cgggtgcgtc catgccgtcc atcggcctgg agcaactgct
     4681 ggcggtcaat cctgcctggc tgctggttgc ccactatcgc gaagagagca ttgttaaacg
     4741 ctggcaacaa gatccgctct ggcagatgtt aaccgccgcg cagaagcagc aggttgcttc
     4801 ggtcgacagt aacacctggg cgcggatgcg cggtattttt gctgcagagc gtattgccgc
     4861 tgacacggta aaaatcttcc accatcagcc gcttaccgtt gtgaaatgac cgcgataaaa
     4921 cacccggtgc tgctgtgggg gcttcccgtt gcagcactta ttattatttt ctggctgagt
     4981 ctgttttgct actcggccat tcctgtttcc ggagcagatg caacccgcgc cctgctgcct
     5041 ggacacacgc caacgctacc agaagcgctg gtgcaaaacc ttcgtttgcc acgaagcctg
     5101 gtcgccgttc tgatcggcgc aagcctggcg ctcgcgggca cgctgctgca aaccctgacc
     5161 cacaacccaa tggcctctcc ttcactgctc ggcattaaca gcggcgcggc gctggctatg
     5221 gcgcttacca gcgcgctgag tccgacgccg attgcaggct attctctgtc gttcatcgcg
     5281 gcatgcgggg gcggcgtgag ctggctgctg gtcatgaccg caggaggcgg atttcgtcat
     5341 acccatgaca gaaacaaact gatcctcgcg ggtatcgcgc tgtcggcctt ttgtatgggc
     5401 cttacccgca tcaccctgct gctggccgaa gatcatgctt acggcatctt ttactggctg
     5461 gcaggcggag tgtcccacgc ccgctggcag gatgtctggc agctcttgcc ggtggtggtc
     5521 actgcagtcc ctgtcgtgtt gctgctggcg aatcaactga acctgctcaa cctcagcgac
     5581 agcaccgccc atacgctggg agtgaacctg acgaggctac gtttggtcat caatatgtta
     5641 gtgctgcttc tggttggtgc gtgcgtcagc gtggcaggtc cggtggcgtt tatcggtctg
     5701 ctggtgccac atctggcgcg cttctgggca ggcttcgatc agcgcaacgt actgccagtg
     5761 agcatgctgc tgggggccac gctgatgctg ctggcagatg tactcgcacg cgcactggcc
     5821 ttccccggag atctgcccgc aggcgcagtg ctggcgctga ttggcagccc ttgctttgtc
     5881 tggcttgtga ggaggcgagg atgaaaattg cgctggttat tttcatcacc cttgccctgg
     5941 cgggctgtgc gctgttatca ctccatatgg gagtgatccc cgtgccgtgg cgcgcgctgc
     6001 tgaccgactg gcaggccgga cgcgagcatt attatgtatt gatggagtac cgactgccgc
     6061 gcttgctgct ggcactgttt gtcggtgcag ccctcgccgt ggcgggcgtg ctgatacagg
     6121 ggattgtgcg caaccctctg gcatcaccgg atattctcgg tgttaaccat gccgccagcc
     6181 tggcctctgt gggggctcta cttcttatgc cgtcactgcc cgtgatggtg ctgccgctgc
     6241 tggcctttgc gggcggcatg gcggggttga tattgctgaa gatgctggca aagacccacc
     6301 agccgatgaa gctggcgctc accggcgtgg cgctttctgc atgctgggcc agcctgacgg
     6361 attatctgat gctctcgcgc ccgcaggatg tgaacaacgc cctgctgtgg ctgaccggca
     6421 gcttatgggg ccgtgactgg agctttgtga agattgccat cccgctgatg attttatttc
     6481 tgccgctgag cctgagtttt tgccgcgatc tcgacctcct tgcactcggc gatgcgcgcg
     6541 ccaccacgct cggtgtgtcg gtgccccata cccgattctg ggctttgtta ctagctgtcg
     6601 ccatgacatc taccggcgtg gccgcctgcg gcccgattag ctttattggt ctcgtggtgc
     6661 cgcatatgat gcgtagcatc accggtggac gtcaccgcag actgctgcct gtttcggccc
     6721 tgacaggtgc gttgctgttg gtggttgccg atctgctggc gagaattatt catcccccac
     6781 tggagctccc ggttggcgtg ctgaccgcca ttatcggtgc gccgtggttt gtctggttgc
     6841 ttgtgagaat gcgataaatg actttacgaa ctgaaaatct gacggtcagt tacgggacag
     6901 acaaggtact taacgacgtt tcactctcac tgccaacggg gaagatcacc gccctgatcg
     6961 gtcctaacgg ttgcgggaaa tcgacgctgt taaactgttt ttcgcggctt ttaatgccgc
     7021 agtctggcac cgtatttctc ggcgataatc ccataaatat gctctcatcg cgccagttgg
     7081 cccgcaggct ttcgctgctg cctcagcacc atttaacgcc agaggggatc acagtccagg
     7141 agctggtttc gtatggtcgt aatccctggc tgtcactctg ggggcgtctc tccgctgaag
     7201 acaatgcacg agttaatgtc gccatgaacc agacccggat caatcatctt gccgttcgtc
     7261 ggttaaccga gctttccggc ggtcagcgcc agcgcgcatt tctggcaatg gtcctggccc
     7321 agaatacgcc cgttgtatta cttgatgagc caaccaccta tcttgatatc aatcaccagg
     7381 tggacctgat gcggttgatg ggcgaactcc ggactcaggg gaaaacggtg gtcgctgtgc
     7441 tgcacgacct taatcaggct agccggtact gcgatcaact ggtggtaatg gcaaacggac
     7501 atgttatggc gcaaggcaca ccagaagagg tgatgacccc aggattgctg agaacagtat
     7561 tcagcgtgga agcggaaata caccccgagc cggtatctgg caggccgatg tgcctaatga
     7621 ggtagattgc acaggccgta agaaccaaac cacgactgaa tgaaactgga ctggcgccag
     7681 caagcctgtt cagactgggg ctgaactttt ccggactctg aaagattacc aatactcatc
     7741 gtccatccgc ttgctttagg ctgacaggtt cataatcaac gcaaaccaga gctgtacagg
     7801 cttgggcgcg gctttcaaac cagtcgtgat cacggcaatc aattttgaac tctgcttaac
     7861 ggacatttct gtataaccct tacggcaacg aaaaacgcga agttaaaatt ttagaaaccc
     7921 aaaaacgtga catgactaag tttagatttc aggggggaga tcaaaaaatt tcgctctgtg
     7981 ccagagcgga cattcacgga gctggttcat taccaatgag gttgggcttt tgaggataaa
     8041 tcaatgatca gacgccaacg taaatcaaaa gcaaccctgg aacggaattg ctaatccagt
     8101 ttctgaccat cgatttttct aaaaagtgtg cgtttgctac tacttaggta ggtgcagctt
     8161 tcttaatcac cggcagccac gttatacagg ccagttgatg gatcgattgt tatcaatgat
     8221 atcttcatga gtcggtgtct cacccagctt accgaagctg gcataaagtg aaggcagacg
     8281 ggcccgtcct tctccctttt tcgccagagg gaaagcgcga agcatggtgg cagcctccca
     8341 gtcacaacaa taggatggtg tgcacggctg ctgacgccat gattcagcga tagagccgga
     8401 aaatacgggg tcaaagccgg tatcattaac cagagtcatc gtgacctgta ctgcggctgg
     8461 atgatctcct gctactgcaa ctgccaggcg accgcggctc ccttcaggta gtctgttgtg
     8521 gtcaactaaa actggcctcc gcgttagagt ttttccagta tcggttttct gattcgtttg
     8581 gtggtaaccc accattatat tcgtgcggtc ttagtgcgct gtaatatcca acgatatagt
     8641 ccgttattgc gtgagctgca tcgctgaa
//
LOCUS       DACADA010000176         8648 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.178, whole genome shotgun sequence.
ACCESSION   DACADA010000176 DACADA010000000
VERSION     DACADA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.178"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(126..1193)
                     /gene="purK"
                     /locus_tag="ISB17_004090"
     CDS             complement(126..1193)
                     /gene="purK"
                     /locus_tag="ISB17_004090"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP1981964.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(1190..1699)
                     /gene="purE"
                     /locus_tag="ISB17_004091"
     CDS             complement(1190..1699)
                     /gene="purE"
                     /locus_tag="ISB17_004091"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP1981965.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(1795..2124)
                     /locus_tag="ISB17_004092"
     CDS             complement(1795..2124)
                     /locus_tag="ISB17_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1981966.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(2235..2957)
                     /gene="lpxH"
                     /locus_tag="ISB17_004093"
     CDS             complement(2235..2957)
                     /gene="lpxH"
                     /locus_tag="ISB17_004093"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAP1981967.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(2960..3454)
                     /gene="ppiB"
                     /locus_tag="ISB17_004094"
     CDS             complement(2960..3454)
                     /gene="ppiB"
                     /locus_tag="ISB17_004094"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAP1981968.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3628..5013
                     /gene="cysS"
                     /locus_tag="ISB17_004095"
     CDS             3628..5013
                     /gene="cysS"
                     /locus_tag="ISB17_004095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP1981969.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(5049..5570)
                     /locus_tag="ISB17_004096"
     CDS             complement(5049..5570)
                     /locus_tag="ISB17_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1981970.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5678..5890)
                     /gene="ybcJ"
                     /locus_tag="ISB17_004097"
     CDS             complement(5678..5890)
                     /gene="ybcJ"
                     /locus_tag="ISB17_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAP1981971.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(5892..6758)
                     /gene="folD"
                     /locus_tag="ISB17_004098"
     CDS             complement(5892..6758)
                     /gene="folD"
                     /locus_tag="ISB17_004098"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAP1981972.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            7029..7105
                     /locus_tag="ISB17_004099"
     tRNA            7029..7105
                     /locus_tag="ISB17_004099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7063..7065,aa:Arg,seq:tct)
     gene            complement(7113..7403)
                     /locus_tag="ISB17_004100"
     CDS             complement(7113..7403)
                     /locus_tag="ISB17_004100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1981973.1"
                     /translation="MRIDSKTAWLSACRRAGVENFRFHDLWHTRASRLIPSGVPLSVL
                     QEMGGWESREMVRRYAHLAPNHLTEHARKIDDIFGDNVPLWNYRRNKEGVTD"
     gene            complement(7441..7740)
                     /locus_tag="ISB17_004101"
     CDS             complement(7441..7740)
                     /locus_tag="ISB17_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1981974.1"
                     /translation="MRWNGTRDLQKNQAKRLIDESPELLKSVVKFALVTVLRKSNIIN
                     LEWQQIDMQRQVARVDPEDSKSNRAIGVALNDTASKVLRDQTGKHHKCGCTYQGG"
     gene            7798..8103
                     /locus_tag="ISB17_004102"
     CDS             7798..8103
                     /locus_tag="ISB17_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1981975.1"
                     /translation="MVVFNSDEASWHLVEDHLGKTVYDVASGDALFISELGPLPENVT
                     WLSPAGEFQKWNGTSWIKDTEEETSLLEAWKMYRVLLNRVDTSTAPDIEWPVNPVRE"
     gene            complement(8158..>8648)
                     /locus_tag="ISB17_004103"
     CDS             complement(8158..>8648)
                     /locus_tag="ISB17_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1981976.1"
                     /translation="GVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKIS
                     HDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILG
                     DGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASG
                     KK"
BASE COUNT         2087 a         2274 c         2232 g         2055 t
ORIGIN      
        1 atccggcaat cggcacacca tgcctgatgc gatgctggtg cgtcttatca ggtctacagg
       61 cggtgttgga acggtaggcc ggataaggcg ttcacgccgc atccggcaat caaatgagca
      121 ccaggttaac tgaacttact ctgcgcccac atcacgccgc tggcatactc cggtggcagc
      181 agcgggatta aggcttccag cgtcgcggtc agatgcgatg tgtcgctgtc ggtcaaattt
      241 aaatgcccca ctttacggcc cggacggact tctttgtcgt accagtgcag atgcacaagc
      301 ggcagtttca gccagtcata attcagatcg ctaccaatca gattgatcat caccgacgga
      361 ttattcacta ctggttgcgg taacggcaga tcggtaattg cccgcagatg tagctcaaac
      421 tggctgatgc tggcaccgtt ttgcgtccag tgcccactgt tatgcacacg cggagccagt
      481 tcgttgatca gcagaccttg cggcgtgaca aaacactcca tcgccatcac gcccacatag
      541 cccagctcct gcataatcgc cgacaacatc tcttcagctt gcgcctgcag ctgtgcgttg
      601 gcctgcggga aagcgacgct ggtgcgcaaa atgccgtcct gatgcaggtt atgcgtcagc
      661 ggataaaaca cggtgctgcc atcaaaaccg cgcgcgccaa ccagcgacac ttcacctgag
      721 aagtttatgc cctgctcgac aatacattcg ccgtagcatt cagccggtaa ctgttcggtt
      781 tcagcggcgc gtaaacgcca ttgaccgcgt ccgtcatagc caccagtgcg gcgcttaaca
      841 atcgccagct cacctaaacg atcaaacacc gcaggccact cgctgcgttc ggcaagtaac
      901 tgccacggtg cggtcggcag gtggagctta tcgaaaagct gcttctgagt cagacggtcg
      961 gcaataatcg ggaacacatc gcggttcaca aaggccggct ggcgcgccag ctcgcgggtt
     1021 aatgcagttt ccggccagcg ttcgatctca gcggtaatca cgctttgttg aaaaggcacc
     1081 gccgccggtt cagcgtccag cccgaccggc cagacagcaa tgcctaacgg ttcgcctgcc
     1141 tgacgcagca tacggcctaa ctgcccgtta ccgaggacgc aaacctgttt catgccgcac
     1201 ctcgcgggtc cgggttttcc agcacttcat cagtttgtgc tttgcgccag tcattcagac
     1261 gctggtgcag ttctttatca tgagtcgcaa gaatttgtgc tgccagtaac gccgcgtttg
     1321 ccgcgccagc tttaccaatc gccagcgtac ccaccggaat gccgcgcggc atttgtacga
     1381 tggagtagag gctatcgaca ccgctcagtg cggcgctctg tactggcacg cccagcaccg
     1441 gcaccagcgt tttggcggca atcatgccag gcaggtgcgc tgcgccgcct gcgcccgcaa
     1501 taatcacctg ataaccgttc tcttcggcgc tttcggcgaa gctgaacagt ttatcggggg
     1561 tgcggtgagc agaaaccact tcaacgtggt gcgggacatt caggatttcg aagatttcgg
     1621 cggcgaactg catggtagcc cagtcgcttt tggaccccat cacgatggcg acacgcgccg
     1681 gattattgcg ggaagacatg cgtcttaaaa ctcctgtggt gcacaactct cggctttaga
     1741 gggcacagag aatagcacgg aaagagtgct aggaaaacgg ttgcgtaggc ggaattaacc
     1801 gtcagttgtt gagtttttaa tttgttctgc gtgttgatta tgcgctctgg ctgaactggc
     1861 aaagaatgcc catatcagca caatgctcca cggtaaaaag catataccga caatcaatat
     1921 atatctttct acaaagcgtt ggttatgggg gcgggtgagt actaaataaa tgagccagaa
     1981 atagactatc caggaaatga ccgccgcagc taccagtgac aatgcgccaa aatagaagcc
     2041 gacaagaccg aaccagctaa tacacagtgg cgctcctgcc gtaagggcga tgccagaaat
     2101 caaaaatccg ttgcagcgtt gcataataat attccctgtt gttatttcac tcagagatta
     2161 ttaccttgct gattttaaaa aagatataca ggttgcgtta gttcggtgct ttttcagaat
     2221 ctgtccattc gtggtcagaa aggaaaatgg attagctcaa cgtcatccgc cgtgactttc
     2281 accatcgaac cttctgtatg ccaggcaccc agtaccacgc gaaaagcagg ttgctgattg
     2341 gcgataagtt catgcaccgc cgggcgatgg gtatgcccgt ggatcagcca ttgcacttga
     2401 tgtttttcca tcgcactgac caccgcgttt tggttaacgt ccatgatcgc cagcgattta
     2461 ctgctgttgg cttctttact gttcgcgcgc attcgcgcgg caatgcgttt gcgcacaaac
     2521 aacggcaggg cgaggaatag cgtttgcagc cagggtttgt ggactttggc gcgaaaagcc
     2581 tgataacccg cgtcatcggt gcacagcgtg tcgccatgca taatcaacac ccgacgacca
     2641 taaagttcga gcaccttttc ttccggcaat aacgtcatgc cgctttcacg ggcaaagcgt
     2701 ttgccgagca gaaaatcacg gttgccatga atgaaataac agggaacgcc ggaatcggat
     2761 accgctttga tcgctgcggc catctggtga tgaagtgggt tgggatcgtc gtcgccgatc
     2821 caggcttcaa acagatcgcc aagaatatac agcgcatcgg ccttgcgggc ctcccctgct
     2881 aaaaaacgca gaaaaccggc ggtgatcgcc ggttcttcca cgcagagatg aagatctgca
     2941 ataaagagtg tcgccacgat tactcgctaa cggtcacgct ttcaatgata acgtcttctt
     3001 ttggcacgtc ctggtgcata ccgctacgac cggttgccac acctttgatt ttgtctacca
     3061 cgtccatgcc ttcaaccact tcagcaaata cgcagtagcc ccaaccttgc aggctttcgc
     3121 cagagaagtt caggaagtcg ttatcaacca cgttgatgaa gaactgggca gttgcagagt
     3181 gcggtgcctg agtacgggcc attgccagcg taccacgggt atttttcaga ccgttgttcg
     3241 cttcgttttt gatcgggtct ttggtggctt tttgtttcat gcccggttca aaaccgccgc
     3301 cctgaatcat aaagccgttg ataacacggt ggaaaatggt gttgttgtaa aaaccttcgc
     3361 ggcagtagtc caggaagttt ttaactgttt caggtgcttt atcgtcaaaa gttttgatga
     3421 caatatcgcc gtgattggtg tggaaagtaa ccatttttgc atcctgttcc gtttgattgg
     3481 tgcttcaacc cggttcgggt catatatagg gtggtgttat agcataaccg cacgatcgga
     3541 tcatctcgca atgtatgctg attcgcgcgg gaaatatggg tattatacgc aactcaatta
     3601 cccacacatg tctaaacgga atcttcgatg ctaaaaatct tcaatactct gacacgccaa
     3661 aaagaggaat ttaagcctat tcacgccggg gaagtcggca tgtacgtgtg tggaatcacc
     3721 gtttacgatc tctgtcatat cggtcacggg cgtacctttg ttgcctttga cgtggttgcg
     3781 cgctatctgc gtttcctcgg ctataagctg aagtatgtgc gcaacattac tgatatcgac
     3841 gacaaaatca tcaaacgcgc caatgaaaat ggcgaaagct ttgtggcgct ggtggatcgc
     3901 atgatcgccg aaatgcacaa agattttgat gctttgaaca ttctgcgccc ggatatggag
     3961 ccgcgcgcga cgcaccatat cgccgaaatt attgaactca ctgaacaact gatcgctaaa
     4021 ggtcacgctt atgtggctga caacggcgac gtgatgttcg acgtcccgac cgatccgaca
     4081 tatggcgtgc tgtcgcgtca ggatctcgat cagctgcagg caggcgcgcg cgttgacgtg
     4141 gtcgacgaca aacgcaaccc gatggacttc gttctgtgga aaatgtcgaa agagggcgaa
     4201 cctagctggc cgtctccgtg gggcgcgggc cgtcctggct ggcacattga atgttcggca
     4261 atgaactgca agcagctggg taaccacttt gatatccacg gcggcggttc tgacctgatg
     4321 ttcccgcacc acgagaacga aattgcccag tccacctgtg cgcacgatgg ccagtatgtg
     4381 aattactgga tgcactccgg tatggtgatg gttgaccgcg agaagatgtc taaatcgctg
     4441 ggtaacttct tcaccgtgcg tgatgtgctg aaatactacg atgcggaaac tgtgcgttac
     4501 ttcctgatgt cgggtcacta tcgcagtcag ttgaactaca gcgaagagaa cctgaaacag
     4561 gcgcgtgcgg cgctggaacg tctctacact gcgctgcgcg gcacagacaa aaccgttgcg
     4621 cctgccggtg gcgaagcgtt tgaagcgcgc tttatcgagg cgatgaacga cgatttcaac
     4681 actccggaag cctattccgt actgtttgat atggcgcgtg aagtgaaccg tctgaaagca
     4741 gaagatatgg cagcggcgaa tgcaatggcg tctcacctgc gtaaactttc tgccgtattg
     4801 ggcctgctgg agcaagaacc ggaagcgttc ctgcaaagcg gtgcgcaggc agacgacagc
     4861 gaagtggctg agattgaagc gttaattcaa cagcgtctgg atgcccgtaa agcgaaagac
     4921 tgggcggcgg cagatgcggc gcgtgaccgt cttaacgaga tggggatcgt gctggaagat
     4981 ggcccgcaag ggaccacctg gcgtcgtaag taattgcgct attgccggat gcgagttttc
     5041 gcatccggtt atcgtctgcg ccaccacaac attcccatca gtaacatccc cggcaaccac
     5101 acccacatca attcagaaat aatcacctga tgcccgtacg gcgtggtgta acgagacaat
     5161 gcaaacggcg cgactttaat cacctgccag ggagcgaaaa agcgttcatc tgaccacggc
     5221 cacagccagc caacccattt accgccagtg gttaccgaat ccagcaagct gtgcgatagc
     5281 aacgagacgg ttaaaaacag ccagcagcga atcagcccag ccctgaacca tcgacgtcca
     5341 atcagcacac ataacagcgg gacaacaaac gcaaacacca gcgaatgggt aaacccgcga
     5401 tgaccaaaaa catttccgta agcaacgcca aatttaaacg acaatacatc ggcatcgggc
     5461 agcatcgcca ggatgattcc ggcaaataac agacgcggag ggatggcttt cgaacccaac
     5521 cctaaaccaa tgcatagggg aacggcggcg tgcgtaataa cggttggcat gatggtcgct
     5581 tcggcaaaat gtcgatgcta tcagcatgga tgaacggggc gtagagggca aaagtctgaa
     5641 aagagaaccg gcctgttgat acaggccggg aaagggatca ggcaacaacc tgtacgctgt
     5701 gacctgcaaa actcactgtc tgaccggcga cgattttgca gcgtttgcgc gtttcaaccg
     5761 caccgtcgac tttcaccagg ccttcggcta tcgcgatttt cgcctgcgcg ccgctttcgc
     5821 tccagccttc cagtttcagc aagtcgcaca gctcaacgtg cggatgttta cctaaagaaa
     5881 atgtcgccat gttactcacc ctgtggatca tgatattcaa cgcacgcctg tagcgtgttt
     5941 tcaatcagcg tggcaaccgt catcgggcca acgccgcccg gaacaggcgt aatgtatgag
     6001 gcgcgtttag ccgcgtcttc aaacacgacg tcgcccacaa ctttgccatt ttccagacgg
     6061 ttgatgccga catcaatcac aattgcgcct tctttgatcc agtcaccagg aataaagccc
     6121 ggcttgccga cggcaacgat caacaaatcg gcattttcta cgtggtgacg cagattttta
     6181 gtgaaacggt gagtcacggt ggtggtgcaa cctgccagca gcagttccat gctcattggg
     6241 cgaccgacga tattcgatgc gccaatcacc acggcgttaa ggccgaaggt atcaatgttg
     6301 taacgctcaa gcagcgtgac gataccgcgc ggggtgcagg gacgcagacg tggcgcgcgc
     6361 tggcacagac gaccgacgtt gtaaggatgg aaaccgtcca cgtctttgtc cggatgaata
     6421 cgttccagca ctttgacgtt atcaataccc gccggtaacg gcagttgaac cagaatgcca
     6481 tcaatggtgt tgtcggcatt cagcgcatcg ataagctcca gcagctccgc ttcgctggtg
     6541 gtttccggga ggtcataaga gcgggagacg aacccgactt cttcacaagc cttgcgtttg
     6601 cttgcgacat aaatttgcga tgcagggttg ctacccacca gcacaacggc cagtcctggc
     6661 gcccgcagtc cggctgcaat acgcgcctga actttttgag caacttcaga gcgcacctgc
     6721 tgcgcaatcg ttttaccgtc aataatcttt gctgccatca gagagaggat tccatctgtt
     6781 acgtaggtcg aaggggatgc gcctattttg tcagaagcgt ggcgcgctgt cagttttcgt
     6841 tttctgaaaa gtaaaacctt ctgtacctgg ctgattcaca cctatcatga taaccgtcgt
     6901 tggtttgccg ccatagtgag ctacatctga tgcggtcgta cacttgttca gcaactggtt
     6961 caaaagccat tgactcagca agggttgacc gtataattca cgcgattaca gcacaccgtg
     7021 gtatcaatgc gcccttagct cagttggata gagcaacgac cttctaagtc gtgggccgca
     7081 ggttcgaatc ctgcagggcg cgccattaaa attcaatcag ttacgccttc tttatttctt
     7141 ctataattcc agagtgggac attatcacca aaaatgtcgt ctattttcct cgcgtgctca
     7201 gtcaaatgat taggcgcaag gtgagcatat ctacgaacca tttctctgga ctcccatccg
     7261 cccatttcct gaagcactga taatgggacg cctgacggaa tcaacctgct tgcccgggta
     7321 tgccagaggt catggaaacg gaaattttca actcctgcac gacgacaagc tgatagccat
     7381 gctgtcttgc tgtcaatgcg catcttcctg agcgcaggca ttgatgttcc atctgctcgc
     7441 ttagccgccc tggtatgtac acccacattt gtgatgcttg cctgtttgat cacgcaacac
     7501 tttagaggcg gtatcgttca gcgccacacc aatggcgcga tttgatttgc tatcttctgg
     7561 atccaccctg gcaacttgtc gctgcatgtc aatctgttgc cactccagat ttatgatgtt
     7621 cgactttctc agaacagtta ccagcgcaaa tttgacgaca gatttcagta gttcggggct
     7681 ctcatcaatc aggcgttttg cctgattctt ttgtaagtct cttgtgccat tccacctcat
     7741 acaggtttgc ctgcaaacag taccgatatt gcaccgccag atattccggc tggcttcgtg
     7801 gttgttttca acagtgatga ggcatcgtgg catctcgttg aagaccatct gggtaaaacg
     7861 gtttatgacg tggcatcagg ggacgcgtta tttatttctg aactcggtcc gttaccggaa
     7921 aatgtcacct ggttatcccc ggcaggggag tttcagaagt ggaacggcac atcatggata
     7981 aaggatacgg aggaagaaac ctcgttgctg gaagcctgga agatgtatcg ggtgttgctg
     8041 aaccgagttg atacatcaac tgcccctgat attgagtggc ctgtgaaccc tgtcagagag
     8101 taatcattgg gattatgccg cagcacgtcg tgcgcaagaa cgtgctgcgt ctggatgcta
     8161 ttttttccct gaagcggaaa acattactac agtaccttta accttggttt taacattctc
     8221 gaaatgctct gagagtatat gtgttaagcc ttcttcggaa tcttttgtgt ttgaaaagat
     8281 gcctttctga ttgtaaatgc gcatcagttt ttgaccgaag ctattgtgca caacgccgtc
     8341 accaagaatt gtggctccgt atagagttcc atcgtcagtt aaggcctgcg ccgcattgcg
     8401 tattacacag ctttttgtag atatatttcc aggcaggcag tgaagaaggt aaaacatgga
     8461 aatggaatca aattgaccat gtaacgccgc gggataagga tcaaaaacat catggctaat
     8521 tttatgttta atttttgatt ccccagccct tgtcgatgcc gcgttcaggc tagcttcgtt
     8581 caaatccatt aaagatatca gactactctc aggtacgtga gtaaggtaaa acccagttcc
     8641 aacgccaa
//
LOCUS       DACADA010000177         8623 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.179, whole genome shotgun sequence.
ACCESSION   DACADA010000177 DACADA010000000
VERSION     DACADA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.179"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..717
                     /locus_tag="ISB17_004104"
     CDS             <1..717
                     /locus_tag="ISB17_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP1981977.1"
                     /translation="AALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVNIRLENKGN
                     NPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSVLPKDRES
                     VFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPLALKWSWSG
                     SEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTGLNGKANSA
                     KVHFYAINDFGGAIEGNASL"
     gene            752..3352
                     /gene="htrE"
                     /locus_tag="ISB17_004105"
     CDS             752..3352
                     /gene="htrE"
                     /locus_tag="ISB17_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HAP1981978.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3369..3941
                     /locus_tag="ISB17_004106"
     CDS             3369..3941
                     /locus_tag="ISB17_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1981979.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            3956..4558
                     /gene="yadL"
                     /locus_tag="ISB17_004107"
     CDS             3956..4558
                     /gene="yadL"
                     /locus_tag="ISB17_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HAP1981980.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4585..5181
                     /locus_tag="ISB17_004108"
     CDS             4585..5181
                     /locus_tag="ISB17_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1981981.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            5233..6486
                     /locus_tag="ISB17_004109"
     CDS             5233..6486
                     /locus_tag="ISB17_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HAP1981982.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VAVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6599..7393
                     /gene="panB"
                     /locus_tag="ISB17_004110"
     CDS             6599..7393
                     /gene="panB"
                     /locus_tag="ISB17_004110"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP1981983.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7405..8256
                     /gene="panC"
                     /locus_tag="ISB17_004111"
     CDS             7405..8256
                     /gene="panC"
                     /locus_tag="ISB17_004111"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP1981984.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8338..8535)
                     /locus_tag="ISB17_004112"
     CDS             complement(8338..8535)
                     /locus_tag="ISB17_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1981985.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLALRLLSKGM
                     SREDVAEMANLPLAEIDKMIN"
BASE COUNT         2431 a         1939 c         1955 g         2298 t
ORIGIN      
        1 gcagcattat gcttcgcaac ctgtgtagct tttagctcgt cagctatagc agatatcgta
       61 atttccggta cacgcgtaat ctataaaagc gaccaaaaaa gtgtcaacat tcgcctggaa
      121 aataaaggca ataacccatt acttgttcag agttggctgg atactggtga tgacaatgct
      181 gagcctggca gtattaccgt cccctttact gcgactccgc ccgtatcacg cattgatgca
      241 aaacgcggcc aaaccatcaa actgatgtac accgccagca gcgtcctgcc aaaagacaga
      301 gaaagtgtgt tctggtttaa cgtactagaa gttcctccaa aaccagatgc cgcaacagtt
      361 gcaaatcaaa gcctgctgca attggcattt cgcacacgta taaaactgtt ctatcgcccg
      421 gaagcattga aggggaatcc ctcagaagca ccattagccc tgaaatggtc ctggtcaggt
      481 tccgagggta aagcagcatt acgagtggcc aatccaaccc cttactacgt ctcttttagc
      541 agtggtgatt tagaagccag cggtaaacgc tttccgattg atgtgaaaat gattgcacca
      601 ttcagtgatg aagtcatgaa agtcactggc cttaatggca aagcaaattc tgcaaaagtg
      661 catttttacg ccattaatga ttttggtggt gccattgaag gtaatgccag tctgtaatca
      721 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
      781 caatcatctg acccgtatcg ccacgttttg cgcgctgctg tattgcaatg gtgctttctg
      841 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
      901 tctcagccgg tatagtgaag gtaaccccgc tataccgggt gtgtacgacg ttagtgttta
      961 tgtcaacgat caacctgtta ttaaccaaag cattacattt gtcgcaattg aaggaaaaaa
     1021 gaacgcacaa gcctgtatca cacttaaaaa tttattgcag ttccatatta attctcccga
     1081 tataaacagc gaaaaagccg ttctgcttgc ccgggatgaa gctctcggca attgcctcaa
     1141 tttgacggaa attatccctc aggcttccgt tcgttatgac gttaacgatc aacgtctgga
     1201 tatagacgtt cctcaggcct gggtaatgaa aaattaccaa aactatgttg atccatcgtt
     1261 atgggaaaat ggcattaatg cagccatgtt gtcatacaac ctcaacggat atcatagtga
     1321 aacccctggt cgaaaaaatg acagcattta tgctgctttt aacggtggga tgaatttggg
     1381 tgcatggcgg ctgcgtgcct caggcaacta caactggatg accgattctg gcggtaatta
     1441 tgattttaag aatcggtata tacagcgtga tatcgcctca ctacgctctc aattcattat
     1501 tggtgagtct tatacgacgg gcgaaacctt tgattccgtc agtatccgag gcattcgctt
     1561 atacagtgac agccgcatgt tgcctccgac attagccagc tttgcgccta tcattcatgg
     1621 cgttgccaat accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac
     1681 gacggtgccg ccaggcgctt tcgtcattga tgatctgagt ccatcagggt acggcagtga
     1741 tcttattgtt accatcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc
     1801 atcggtcgtt caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt
     1861 cttaaaagat gatattcagg atgagcctaa tttatttcag gccagctact actacggtct
     1921 gaataactat ctgacgggtt ataccggtat tcagattacc gataataact ataccgctgg
     1981 tttgttaggt cttggtctga atacttcggt cggtgcattt tctttcgatg tgactcactc
     2041 caatgttcgt ataccggatg ataaaacata ccaggggcaa agttatcgtg tttcctggaa
     2101 caagttattc gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca
     2161 gaattatctt ggtcttaatg atgcactaac tctgattgat gaagtgaaac atccagaaca
     2221 agatcttgaa ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacagtcag
     2281 tattaaccaa ccgttgaaat ttgaaaaaaa agattacggt tctttttatc tttctggtag
     2341 ttggtccgat tactgggctt ccggacaaaa tcgtagcaat tactccattg gctacagtaa
     2401 cagtgcatcc tggggcagct acagcatcag tgcacagcgt tcatggaatg aggacggcga
     2461 cgctgatgat agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga
     2521 acaacgtact tcaggtttcc agagtatcga tactcaaatg agcagtgact ttaagggtaa
     2581 taaccaactc aacgtcagta gcagtggcta tagcgataac gctcgcgtca gttatagcgt
     2641 gaatactggc tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc
     2701 cagctatgaa tcaccgtggg gaactctggc aggttcagtt tctgcaaaca gcgataacag
     2761 tcgtcaagtt tctctcagca ccgacggtgg ttttgtattg catagtggcg gcctgacctt
     2821 cagtaatgac agttttagcg actccgatac actggcggta gttcaggctc caggtgctca
     2881 aggagcgcga ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag
     2941 cgctctttct ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga
     3001 tgttgaatta aaaagtacca gtgctgtagc tgtgcctcgt cagggttctg tcgtttttgc
     3061 tgattttgaa accgtgcaag ggcaatcagc cattatgaac atcacacgta gtgatggcaa
     3121 aaatattcca tttgctgcag atatttatga tgaccaaggc aatgtcattg gtaatgttgg
     3181 acagggtgga caggcttttg ttcgtggtat tgaacagcaa ggaaatatca acattaaatg
     3241 gctcgaagaa agtaaacctg taagttgtct tgcgcattat caacaaagtt cagaagcaga
     3301 aaaaatagcg caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg
     3361 gacgtcaaat gataaaaaca acgccacata aaatagcgat actgatggga gtattattat
     3421 caccctcagt atttgcaacg gatattaatg ttgagtttac agccactgtc aaagcgacaa
     3481 cctgtaacat cacacttact ggtaataatg tcacgaatga tggcaataat aactacactt
     3541 tgagaatccc taagatgggc ctggataaga tcacgaataa aacgacagaa tctcaggctg
     3601 attttaaact ggttgccagt gggtgcagca gtggcattag ctggattgat accactctga
     3661 ccggaaacgc attatcaagc tcatccaagc ttattatacc gctgtctggt gatttatctt
     3721 cgacgacaag taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc
     3781 ttcaacctaa caacagtgcg gacaagatac gctggagcac agaagaaatg cagcccgata
     3841 aaggtcttga aatgactgtc gcgctacgtg aaaccgcagc agggcaaggc gtgccgggga
     3901 tttttcgcgc cctggcgacg tttaatttca tctatcaata agaaggataa ttaagatgac
     3961 atttaaaaat ttacgttatg gattatccag cagtgtcgtt ttggctgcct cattgttcag
     4021 cgtaccctct tatgcggcaa ctgacagcat tgggctgacc gtgattacca ccgtagaaat
     4081 gggtacttgt accgctacat tagtaaatga ctctgatcag gacatttctg ttgttgattt
     4141 tggtgatgta tatatttctg aaatcaatgc caagaccaaa gtaaaaacat tcaaactcaa
     4201 attcaaagac tgtgcgggta tccccaataa aaaagcgcaa ataaaattaa ccaagcgagc
     4261 cacatgcgag gggactgcta atgacggtgc agggttcgca aatggttcca cagccacaga
     4321 taaagcaagt gccgtcgccg ttgaagtctg gagcaccgaa actccggcaa cagggagagc
     4381 aacacaattt agttgtgtga caccagcatc tcaagaggta actatttcca ctgctgccaa
     4441 tgcggttgtt tattacccga tgagtgctcg cctggtcgtt gaaaaaaata aaaccgtaag
     4501 taatgtcact gcaggtaaat tttctgcacc tgccacattt acagtaacct ataactaaca
     4561 gactgacaga ttcaggaact atatatgcat cccactcaac gtaagctgat gaagagaata
     4621 attctgtttc tgtcattact gtttcccatt acctgtccag cgatcgctgg acaggatatt
     4681 gaccttgttg ctaatgtaaa aaacagcact tgcaaaagcg gaatcagtaa ccagggtaat
     4741 attgatcttg gtgtcgttgg ggtgggatat ttttcaggta atgttactcc tgaaagctat
     4801 caaccaggtg gaaaagagtt cactgtcact gtctcagact gtgcattaca gggaactggc
     4861 gatgtgctaa atcagttaca tattgatttt agagccctta gcggtgtcat ggctgcaggc
     4921 tccaggcaaa tatttgccaa tgaagtgaca accggggcta aaaatgtcgg tgtcgttctc
     4981 ttttccattc aggacccggc caatatattc aatgttctta gttcagcggg aaattctcgt
     5041 tcaatctatc cagtaatgac ttctgccctg aataattcgt catggaaatt ttatgccaga
     5101 atgcaaaaaa tcgatcccgc gctggatgtc atatctggcc aggtgatgag tcatatatta
     5161 gttgatgtgc actacgaata atatatcaaa acaaacgact gctattatga cagcagcgaa
     5221 tagtgggtaa tcatgaagac attcttcaaa tactttgttt ttttagtact atcctgctct
     5281 tgctttacag cgattagtaa ggatacaagt tttcttgttg gtaacaatgc cggggtaggc
     5341 gatggtaatt tcaacggccc gagcactgcg gctcaggtca cttttagata tgcatcaaca
     5401 accaataatt tagtttttta taaacctact caacttggcc cgacaggtgt taaattacgc
     5461 tgggaacaat tagatacagc cagcggtggc ggttttcttt actgtaatgc ctctgacagg
     5521 gcaaatccag atggactaat ggatattgaa aatgccatgg ctgattctgg taaaacctat
     5581 ggtggacata agttatttaa aacctctgtc ccaggcttgt attacactct atttattgca
     5641 aaactttggt ctgcatattc aacaaaaaca actattagcg actctggttt atatattgga
     5701 gataccacac cacaaaaatt ccagtggatc atcacagacc ctgatctaca acatatcggc
     5761 tgcgataacg cgatgcggta cgatagattc tgggccatcg gcggtgttgt acacgatctt
     5821 acgattgaat tttatactga taccgatttt aaccctacaa caaaccagga cgtatcattg
     5881 tccagtaatt cgacatatct ttatgcgttt aaggcctaca gtcccggttc tcgtgttgtt
     5941 gatcacagcc atcaccttta tatcgacttt gatctactca atgttaaatt aactaacccc
     6001 acctgcttta ctgctgtact taccggaaaa tcagtaagtg gttcgacggt aaaaatggga
     6061 gaatatgcac cagggcagat taaaaatggt gctactccgg ttccttttga tatttctctc
     6121 cagaactgtg ttcgcgttgg cgatatagag acaaagctat cttcagggaa gctgggtaca
     6181 gaaaataagc aactcctggg taacactctt actggaagcg gcgcagcaaa aggcgttgcg
     6241 gtattgattg aaggattagc aaacagaaaa agtgccctaa tgatactgaa accgaatgat
     6301 tcaacttctg tttataaaga taatacgggt caggctcagg atcacgattc agatgcaatt
     6361 tacccagaag atcaaggtat aacatatcca cttcatttcc aggcgacgtt aaagcaagac
     6421 ggaaatattg ctatcgaacc aggagaattt aaagccacca gtactttcca ggtaacctat
     6481 ccctgataac attgtggcac cgtctggtaa tggcggtgcc acaatagata aatcattgcc
     6541 cccaccaatg tggctgataa aatgccagac agcacgacat tacttatcag gatacgttat
     6601 gaaaccaacc actattgctt cattgcaaaa atgcaaacaa gaaaaaaaac gtttcgcgac
     6661 catcactgct tacgattata gcttcgcaaa actgtttgcc gaagaaggac ttaacgtcat
     6721 gctggtcggc gattctctgg gcatgacggt tcaggggcat gactccacgc taccggttac
     6781 cgttgctgat atcgcctacc acactgccgc cgtgcgtcgc ggcgcgccaa actgccttct
     6841 gttagcagac ctgccattta tggcgtatgc cacgccggaa caagcctttg aaaacgccgc
     6901 aacggtaatg cgtgccggag ccaacatggt caaaattgaa ggcggtgagt ggctggtaga
     6961 aaccgtacaa atgctgaccg aacgtgccgt tccagtttgt ggtcatttag gcctaacccc
     7021 acagtcagta aatattttcg gtggctacaa agtccagggg cgcggcgatg aagcggccga
     7081 tcgactgctc agcgatgcat tagccttaga agctgctggg gcacaactgc tggtactgga
     7141 gtgcgtgccg gttgaactgg cgaaacgcat taccgaagca ctggcgatcc cggttatcgg
     7201 tattggcgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg cgttcggcat
     7261 taccggcggt catattccta aattcgccaa aaatttcctc gccgaaacgg gcgacatccg
     7321 cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg gcgaagaaca
     7381 cagtttccat tgaggagtga cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag
     7441 caaatccgcc gcctgcgtat ggaaggtaag cgcgtggcgc tggtgcccac catgggtaac
     7501 ctgcacgacg gccatatgaa gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc
     7561 gtcagtattt tcgttaaccc aatgcagttt gaccgcccgg aagatctggc acgttatcca
     7621 cggaccttgc aagaggactg cgaaaagctg aacaaacgta aagtggattt agttttcgcg
     7681 ccttcggtaa aagagatcta cccgaacggt acagaaaccc acacctatgt tgatgttcct
     7741 ggtctttcca ccatgctgga aggtgccagc cgtccgggac attttcgcgg cgtttcgact
     7801 attgtcagca agctgttcaa cctggtccag ccggacatcg cttgcttcgg cgaaaaggac
     7861 tttcagcaac tggcgctgat ccgcaaaatg gttgccgata tgggctttga tattgagatt
     7921 atcggcgtac cgattatgcg cgccaaagac ggtctggcgc tgagttcccg taacggttat
     7981 ctgacggcgg aacaacgcaa aattgcgccc ggtctgtaca aggttttaag ttcgattgcc
     8041 gacaaattgc aggctggcga acgggatctc gatgaaatta ttgccattgc cgggcaagaa
     8101 ctgaatgaaa aaggcttccg cgccgatgat attcagattc gtgatgccga cacattgctg
     8161 gaagtctctg aaaacagcaa acgcgcagtg attctggtag ccgcctggct tggcgatgct
     8221 cgcctgatcg acaacaaaat ggtcgaactg gcgtaatgct taactggcgc tacggctgat
     8281 ggcgccagct attcattgcc acgttatctg taagcgatta ttcataacgc agacaactta
     8341 gttaatcatt ttatcaatct cagcaagagg taaatttgcc atctctgcaa cgtcttcccg
     8401 agacattcct ttactcagaa gacgcagggc cagtcttacc ttctggagtc cctctttata
     8461 tcccttctga aatcccttct cttcaaacca ctgcgccaac gtctgcctgg tttctctccg
     8521 tgtctttatt tccatatgtt cccttaatta tctggtcagt taacaataac aattatctgg
     8581 ctataactga taatgcagat aacttagcta atcagcttat caa
//
LOCUS       DACADA010000178         8508 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.180, whole genome shotgun sequence.
ACCESSION   DACADA010000178 DACADA010000000
VERSION     DACADA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.180"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            177..2555
                     /gene="ppsA"
                     /locus_tag="ISB17_004113"
     CDS             177..2555
                     /gene="ppsA"
                     /locus_tag="ISB17_004113"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAP1981986.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2612..4312)
                     /gene="fadK"
                     /locus_tag="ISB17_004114"
     CDS             complement(2612..4312)
                     /gene="fadK"
                     /locus_tag="ISB17_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAP1981987.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKTGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(4315..4608)
                     /locus_tag="ISB17_004115"
     CDS             complement(4315..4608)
                     /locus_tag="ISB17_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP1981988.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4605..5894)
                     /locus_tag="ISB17_004116"
     CDS             complement(4605..5894)
                     /locus_tag="ISB17_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1981989.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5949..6887)
                     /locus_tag="ISB17_004117"
     CDS             complement(5949..6887)
                     /locus_tag="ISB17_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAP1981990.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(6907..7671)
                     /locus_tag="ISB17_004118"
     CDS             complement(6907..7671)
                     /locus_tag="ISB17_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAP1981991.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7987..>8508
                     /locus_tag="ISB17_004119"
     CDS             7987..>8508
                     /locus_tag="ISB17_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP1981992.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQG"
BASE COUNT         2033 a         2190 c         2206 g         2079 t
ORIGIN      
        1 ttatgtggaa attgtcgaag agagcagatt tgcgcaacgc tgggatcagt cttaaaaagt
       61 aaaaaaatat atttgcttga acgattcacc gtttttttca tctggttaaa tatgcaaaga
      121 taaatgcgca gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt
      181 ccaacaatgg ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag
      241 acagggttgg gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg
      301 gtgtttccgt tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc
      361 aaagcggcgt aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta
      421 cccagcttgc gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc
      481 ctgagctgga aaacgccatc cgcgaagcct atgcacagct ttccgccgat gacgaaaacg
      541 cttcgtttgc ggtgcgttcc tccgctactg cagaagatat gccggacgct tcttttgccg
      601 gtcagcagga aactttcctc aacgttcagg gtgttgacgc cgttctcgtg gcagtgaagc
      661 atgtattcgc ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg
      721 accatcgtgg cgtagcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat
      781 cttctggcgt gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca
      841 cttccgcatg gggccttggt gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt
      901 acgtccataa accgacactt gcggcgaatc gcccggctat cgtgcgccgc accatggggt
      961 cgaaaaaaat ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg
     1021 aagacgtacc gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac
     1081 tggcaaaaca ggccgtacaa attgagaaac actacggtcg cccgatggat attgagtggg
     1141 cgaaagatgg tcacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct
     1201 cacgcggtca ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag
     1261 gccgtgctat cggtcatcgc atcggcgcgg gtccggtgaa agttatccat gacatcagtg
     1321 aaatgaaccg catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg
     1381 aaccgatcat gaagaaagca tctgccatcg tcaccaatcg tggcggtcgt acctgtcacg
     1441 cggcgatcat cgctcgtgaa ctcggcattc cggcggtagt gggctgtggt gatgcaacag
     1501 aacggatgaa agacggcgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg
     1561 tctatgcgga gttgctggaa tttagcgtta aaagctccag cgtagaaacg atgccggacc
     1621 tgccgttgaa ggtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc
     1681 tgccgaacga aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg
     1741 tccacccacg cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc
     1801 gcgagatgat gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag
     1861 ggatcgcgac gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt
     1921 ttaaatcgaa cgaatatgcc aatctggtcg gtggtgagcg ttacgagcca gatgaagaga
     1981 acccgatgct cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct
     2041 tcgcgctgga gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg actaacgttg
     2101 agatcatgat cccgttcgtg cgtaccgttg atcaggcgaa agcggtggtt gaggaactgg
     2161 cgcgtcaggg gctgaaacgt ggtgagaacg ggctgaaaat catcatgatg tgtgaaattc
     2221 cgtccaacgc cttgctggca gagcagttcc tggaatattt cgacggcttc tcaattggct
     2281 caaacgacat gacgcagctg gcgctcggtc tggatcgtga ctccggcgtg gtgtctgaac
     2341 tgttcgatga gcgcaacgat gcggtgaaag cactgctgtc gatggcgatt cgtgccgcga
     2401 agaaacaggg caaatatgtc gggatttgcg gtcagggtcc atccgaccac gaagactttg
     2461 ctgcatggtt gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc
     2521 aaacctggtt aagcctggct gaactgaaga aataaaataa atacccggcg gcgttttgtc
     2581 gccgggttta tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgag
     2641 ttaatcgccg cataatatct ttccttaaca aaaacttttg tattttacct gagacagtgc
     2701 gcggtagttt ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac
     2761 ggctaaaaaa agccaccacc tcttccagcg ataatgaatg atgcggcgct ttcagcacga
     2821 cataagcgca tgaacgttca cctaaacgtt catcgggcat cgcaaccaca caggcatcgt
     2881 gaattttagg atgctgcaat aaaatatctt ctacttcacg gctgctaata ttttcgccgc
     2941 cgcggacaat aatatctttc ttgcgcccgg ttatttttat atagccagcc tcatccatgc
     3001 ggcagagatc gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag
     3061 gttcatcaaa ataccccata aacacattgg ggccacgcga ggcttcttca ccttcgcaac
     3121 ctggcggtaa agtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt
     3181 aaccgtcggt gtgcataaag cgcggcaaag gatcgtcgag attcaccacc gcatgcggcg
     3241 aactttctgt ggaaccataa acacttaata atttaatgcc gcgctgctgg cattctcgcg
     3301 ccactttttt ggggattgtg gtacctccgc aaagaaagaa gcgcagcgct gaaaggtctg
     3361 cgggttgttt ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg ccgagcatac
     3421 aggtgcaacg ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca
     3481 acacgctgcg agcaccaatc aaaaaaggtg ctgttacgcc atgcagaaag cccgttgcgt
     3541 gaccaagtgg cgcaggcatc ataaagacat cctgccaggt cagatttagc cgcgcgcaat
     3601 aagcccgctc actggcgaga atattgttat gcgttagcat cacacccttt ggcagaccct
     3661 cggttccgga ggtaaatagc acagcggcta attcatcgcc gtgggtcgtt atcgccgtgg
     3721 tcagaggagt attgtcggcg agaatctgac ttaatgagag ggaagaggtg gcgggagcca
     3781 gtttgtccac gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta
     3841 aatctaccgg acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact
     3901 tattgagcac ccacaccagt tctgcttccc gccaggaagg taacagcggc acggaaaccg
     3961 caccggtttt caggcaggca agatagataa cggtaaattc acaccagcct ggcagttgaa
     4021 atgcgatgcg atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg
     4081 cggcgtgatc gagcgcgcta taggtgtacg ttgcaccatg attatcgacc acggcaattt
     4141 tgtctggcat cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca tcgccccata
     4201 acccttgctg acgatacgcc gcacgacgtt gttcgttgaa cgttaatgtc actttcattt
     4261 tggactctcg aaacagaaca tcaggcccga gatgcgggcc tgtgggatgc atgattagcc
     4321 gtagcgaaat tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt
     4381 gccgcacagc acccgacagg tgccgcactc cagacaaccg gcggaatcga aatgaatgtt
     4441 tcctgcgtca tcctgcttat aaagtccggc agggcaagct ttcattaatt tacggaattc
     4501 attgatatcg ggatttgccg ccaaaatgat atgcgggtgg ccctcatcaa catggaattt
     4561 atcgacgcct aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct
     4621 cccttaatgc catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc
     4681 attttgcgta ccggctggtt tggtttgccg tcaatggtga acatatcgtt catgatgtcg
     4741 gcgaccattc gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg
     4801 cggaaatgct gcatatcgcg cataacacaa ctttgttcca gctcacgttt gtattgcgcc
     4861 agactgctgg cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc
     4921 tgagctgatg caatggctaa atccatgccg cgaactgtaa aacccaaatt caggcagaag
     4981 cctgcggcgt caccaacgat catcacgcca tcgttaacca gctgcggcac cattgccaga
     5041 ccgccctccg gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg
     5101 cgaatagcgg ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg
     5161 gcgatatcac ccaatccaca aaccagcccc aacgatacgg aatccttgtt ggtatagagg
     5221 aatccgccgc ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc
     5281 tcgcccgtaa cattaaagcg atcgttgatc tgttctggtg tcaggcctat aacctcttta
     5341 acaccaacag cgtaatgatg cggattggaa gcggaaacca ttcccagcga gcggccaagc
     5401 atcgagttaa cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccggcc
     5461 tggacgccag tgaccttgtt tccttcacga accagcgcat cgacgcgtac gcccgggata
     5521 aactgcgcgc ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat
     5581 acggtataag acgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg
     5641 gcgctttctt cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct
     5701 gatgctgcaa agcctggaat aattgcttca agtgtgtggg cataaagacg cccgccggtc
     5761 atgtttttac atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgc
     5821 gccatgacca gtgcggcaac gctaccggca acgcccgcac cgaccacaat ggcatcaaat
     5881 ttgtcatccg acatgcgtta tgctcccttc ccgctgcgtt tacagcggga gatgtggttg
     5941 aaagggaatt agcgattcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
     6001 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt aatggcgaca
     6061 atcacttttg cgccgttgcc gccaaccata tgctggatct gcccggagat ccccagcgtc
     6121 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
     6181 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag cactgccgcc
     6241 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
     6301 cgttttgctt tgctcaggtc cacgctgctt gccgctttcg cacggcgttc gcgacagaga
     6361 atttcatgac gcggggcaac ataaggtacc gtttctgtcg ggcactgatg agagatatca
     6421 cgggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
     6481 ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
     6541 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
     6601 gcggccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
     6661 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtac gttgcagcgc gtcgttttgc
     6721 tcaagaacat aaatgcattt tggcccataa ggcataactg cctgcgcctg ctcggtattt
     6781 tgtacaatgg catacacctg ttggccccat tgctgagcgc tgccaaacag ttcagcataa
     6841 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
     6901 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
     6961 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccggtcag
     7021 ttcagcaagt ggtgcgctct ggctccagcc aatatcactt gcctgccact gatttaccgg
     7081 ttttttaccc gcgccgagaa tggctttcat cgaaggaata cgtggcacgt taatatcgga
     7141 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
     7201 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
     7261 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
     7321 ttcaccaaag atcagtaaat cgaagccgat cttttcaact gctgtcgcca gcgcctttgc
     7381 ggtatcgaga ggcagtgcat gctcaagttg tgcatcctgc accatataca ggctgtgcgg
     7441 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaaagagc cgccgacggt
     7501 cagcgcggct atctcatcgt catctgttgc aagctggctt gcagcttcaa tggcattgag
     7561 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
     7621 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
     7681 tgtgagtcgc atacggaacc atctccgatg gaaagtaaaa atggcctgaa aatagtgtgc
     7741 gatagcggtg cggggttttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
     7801 aaaatgcgat cgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
     7861 ctatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
     7921 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
     7981 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
     8041 acgccacggc tttcacgttt cgcatttagc gatgatccaa aatgggagtc tggacatcac
     8101 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggtagcaag gtttaccatc
     8161 gattcttcgt tatatgtcgc gcatgccgat gacattgtgg tgatagaacg tggcaggttg
     8221 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
     8281 tttcagtttc agggggctga ggaaaatcag ctactgcaac cgcattcttg cccggtaatt
     8341 gccgcaggac agggaaaaga agtcattaaa accttattta atgagctaag tgtgattttg
     8401 ccgcaaagta aaaatagcca aacatcttcg ttatgggacg catttgccta tacgttagca
     8461 attctttact acgaaaactt taaaaatgct tatcgttcgg agcaggga
//
LOCUS       DACADA010000179         8485 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.181, whole genome shotgun sequence.
ACCESSION   DACADA010000179 DACADA010000000
VERSION     DACADA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.181"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            80..1201
                     /gene="ompC"
                     /locus_tag="ISB17_004120"
     CDS             80..1201
                     /gene="ompC"
                     /locus_tag="ISB17_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP1981993.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1313..2368
                     /gene="apbE"
                     /locus_tag="ISB17_004121"
     CDS             1313..2368
                     /gene="apbE"
                     /locus_tag="ISB17_004121"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAP1981994.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2442..3506
                     /gene="ada"
                     /locus_tag="ISB17_004122"
     CDS             2442..3506
                     /gene="ada"
                     /locus_tag="ISB17_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAP1981995.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3506..4156
                     /gene="alkB"
                     /locus_tag="ISB17_004123"
     CDS             3506..4156
                     /gene="alkB"
                     /locus_tag="ISB17_004123"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAP1981996.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4232..5875
                     /gene="yojI"
                     /locus_tag="ISB17_004124"
     CDS             4232..5875
                     /gene="yojI"
                     /locus_tag="ISB17_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAP1981997.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6093..7739
                     /gene="mqo"
                     /locus_tag="ISB17_004125"
     CDS             6093..7739
                     /gene="mqo"
                     /locus_tag="ISB17_004125"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAP1981998.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7889..8377)
                     /gene="eco"
                     /locus_tag="ISB17_004126"
     CDS             complement(7889..8377)
                     /gene="eco"
                     /locus_tag="ISB17_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAP1981999.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2041 a         2120 c         2287 g         2037 t
ORIGIN      
        1 gccgactgat taatgagggt taatcagtat gcagtggcat aaaaaagcaa ataaaggcat
       61 ataacagagg gttaataaca tgaaagttaa agtactgtcc ctcctggtcc cagctctgct
      121 ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa gacggcaaca aattagatct
      181 gtacggtaaa gtagacggcc tgcactattt ctctgacaac aagtcagaag acggcgacca
      241 gacctacatg cgtcttggct tcaaaggtga aactcaggtt actgaccagc tgaccggtta
      301 cggccagtgg gaatatcaga tccagggcaa cagcgctgaa aacgaaaaca actcctggac
      361 ccgtgtggca ttcgcaggtc tgaaattcca ggatgtgggt tctttcgact acggtcgtaa
      421 ctacggcgtt gtttatgacg taacttcctg gaccgacgta ctgccagaat tcggtggcga
      481 cacttacggt tctgacaact tcatgcagca gcgtggtaac ggcttcgcga cctatcgtaa
      541 caccgacttc ttcggtctgg ttgacggcct gaactttgct gttcagtacc agggtaaaaa
      601 cggtagcgta gatggcgaag gcatgaccaa caacggtcgt ggtgccctgc gtcagaacgg
      661 cgacggcgtt ggcggatcta tcacttatga ttacgaaggc ttcggtatcg gtgctgctgt
      721 ttctagctcc aaacgtactg atgatcagaa cttcggcctg aacggttatg gtgagcgtta
      781 tttgggtaac ggcgaccgtg ctgaaaccta cactggtggt ctgaaatacg acgctaacaa
      841 catctacctg gctgctcagt atacccagac ctacaacgca actcgcgtag gttccttggg
      901 ttgggcaaac aaagcacaga acttcgaagc tgttgctcag taccagttcg acttcggtct
      961 gcgtccgtct gtagcctacc tgcagtctaa aggtaaaaac ctgggtgtca tcaatggtcg
     1021 taactacgac gacgaagata tcctgaaata tgttgatgtt ggtgcgacct actacttcaa
     1081 caaaaacatg tccacctacg ttgactacaa aatcaacctg ctggacgaca accgcttcac
     1141 ccgtgacgct ggcatcaaca ctgatgacat cgtagctctg ggtctggttt accagttcta
     1201 atctcgattg atatcgaaca aagggcctgc gggccctttt ttcattgttt tcagcgtaca
     1261 aacacagttt tttggtgtac tcttgcgacc gttcgcatga ggataatcac gtatggaaat
     1321 aagctttacc cgcgtggcac tgctggctgc cgcgctcttc tttgttggtt gcgatcaaaa
     1381 accacaaccc gccaaaaccc acgctactga agttaccgtt cttgaaggca aaaccatggg
     1441 taccttctgg cgtgccagca tcccgggcat tgacgccaaa cgcagtgccg aacttaaaga
     1501 aaagattcag acccagctgg acgctgacga tcagctgctt tcgacctata aaaaagattc
     1561 cgcgctgatg cgctttaacg actcgcaaag tttgtcgccg tggccggtaa gtgaagcgat
     1621 ggccgatatc gtcaccacct cgctgcgcat tggcgcaagg accgatggtg cgatggatat
     1681 aaccatcggg ccgctggtga atctgtgggg ctttggcccg gaacaacaac cggttcaaat
     1741 tccgagccag gaacagatcg atgcgatgaa agccaaaacc ggcttacagc acctgacggt
     1801 cattaatcag tcgcatcagc aatatctgca aaaagacctg ccggatttat atgtcgatct
     1861 ctccaccgtc ggcgaaggtt atgcggcgga tcacctggca cgcttgatgg agcaggaagg
     1921 gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac agccgtggta tgaacggtgc
     1981 aggccaaccg tggcgggtag cgattcaaaa accaaccgat aaagaaaacg cggttcaggc
     2041 cgtggtggat atcaacggtc atggtattag cacctctggc agctaccgta actattacga
     2101 actggacggc aaacgtcttt ctcatgttat cgatccgcaa accgggcgtc ctatcgaaca
     2161 caatctggta tccgtgacgg tgattgcccc gacggcgctg gaggccgatg cctgggatac
     2221 tggcttgatg gtactcggac cggagaaagc caaagaggtt gttcgccggg aaggactggc
     2281 ggtctacatg atcaccaaag agggcgataa ttttaaagcc tggatgtcgc cgcagtttaa
     2341 aagcttcctt atcagcgaaa aaaattaaag cgcaagattg ttggtttttg tgcgatggtg
     2401 accgggcaga ctaaaggcta tccttaacca gagagctgat tatgaaatac gccatacgct
     2461 taactgacga tcaacgctgg caatctgtct tagcccgcga ctcgaatgcc gacggcgaat
     2521 tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc gtcttgccga gccagacatg
     2581 ccttgcggga aaatgtctcc ttctacgcaa atgccagcga ggcgcttgcc gctggctttc
     2641 gtccctgcaa acgttgtcag ccagacaaag ccaatcccct gcaacatcgc cttgataaaa
     2701 ttacccacgc ctgccgactt ctggaacagg aaacgcctgt aacgctggaa gccttagccg
     2761 accaggtggc gatgagtcca tttcatttgc atcggttgtt taaagcgact accggaatga
     2821 cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt gcgcgaattg ctggcgaaag
     2881 gggagagcgt gactacatct attcttaacg ccggattccc cgacagcagc agttactacc
     2941 gcaaagccga cgaaacgctg ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc
     3001 tggcggtgcg ttacgcgctg gctgattgtg agctgggccg ttgcctggtg gcagaaagtg
     3061 agcgggggat ttgcgcgata ttactgggcg atgatgacgc gacactaatc agcgagttac
     3121 agcagatgtt tcccgctgcc gacagcgcgc ctgctgatct gacgtttcag caacatgtgc
     3181 gtgaagtgat cgccagcctc aatcaacgcg atacgccgct gacgttaccg ctggacattc
     3241 gcggcactgc ttttcagcaa caagtctggc aggcactgcg cacgatacct tgcggtgaaa
     3301 ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc gaaagcggta cgggctgttg
     3361 ccagcgcctg tgccgccaac aagctagcta tcgttatacc ctgtcatcgg gtagtacgcg
     3421 gtgatggatc gctttcaggt taccgctggg gcgtgtcgcg taaagcgcaa ctgctacgcc
     3481 gcgaagctga aaatgaggag aggtaatgtt ggatctgttt gccgatgctg aaccgtggca
     3541 agaaccactg gcggctggag cggtgatttt gcaccgtttt gcttttaacg ctgcggagca
     3601 actgatccgc gatattaatg acgttgccag ccagtcgccg tttcgccaga tggtcactcc
     3661 aggcggatat accatgtcgg tggcgatgac caactgtggg catctgggct ggacgaccca
     3721 tcggcaaggt tatctctatt cgcccattga tccgcaaaca aataaaccgt ggcccgccat
     3781 gccgcagagt tttcatcatt tatgtcaacg tgcggctacg gcagcgggct atccagattt
     3841 ccagccagat gcctgtttga tcaaccgcta cgttcctggc gcgaaactgt cgctgcatca
     3901 ggataaagac gaaccggatc tgcgcgcgcc aattgtttct gtttctctgg gcttacccgc
     3961 gatttttcaa tttggcggcc tgaaacgaaa tgatccgctc aaacgtttgt tgttggaaca
     4021 tggcgatgtg gtggtatggg gtggtgagtc gcggctgttt tatcatggca ttcaaccgtt
     4081 gaaagtgggg gggcatccac tcaccaccga ctgccgctac aacctgacat tccgtcaggc
     4141 aggtaaaaaa gaataaaaat aagaattatt attgctgtgc gcacgaagat gtttaaactg
     4201 cgggctgtaa ttcattgtcc gggttttctg catggaactt cttgtacttg tctggcggca
     4261 gtatcgctgg ccatttatca gtgtgatggc gctaagcctc gccagtgcgg cattaggcat
     4321 tggcttaatt gcttttatca atcagcgcct tatcgaaacg gcggatacca gtctgctggt
     4381 attgccggag tttctggggt tattgctgct gttgatggca gtcactctcg gatcgcaact
     4441 ggcgctcacc actttggggc atcacttcgt ttaccgactg cgtagtgaat ttatcaagcg
     4501 gattctggat acccatgtcg agcgcattga acaacttggt agcgcctcgt tgctggcggg
     4561 gttaaccagc gatgtgcgca atatcaccat tgcttttgtg cgtctgccgg aactggtgca
     4621 ggggatcatt ctcactatcg gttcagcggc gtatttgtgg atgctgtcgg gcaaaatgtt
     4681 gctggtaacg gctatctgga tggcagtcac catctggggt ggttttatac tggtggcgcg
     4741 ggtgtacaaa catatggcga ccctgcgtga aaccgaagat aagctgtaca cggattttca
     4801 aaccgtactc gaagggcgca aagagctgac tctgaaccgt gaacgcgccg agtatgtgtt
     4861 taacaacctc tacattcctg atgcgcaaga gtatcgccat catattatcc gcgcagacac
     4921 cttccatctt agtgccgtga actggtcaaa catcatgatg ctgggcgcaa tcggcctggt
     4981 gttctggatg gcgaacagtc tcggttgggc tgataccaac gttgccgcga cctattcgtt
     5041 gacgttgtta ttcctgcgta cgccgctgct ttcggcggtt ggcgcgttgc cgacgctact
     5101 gacggcgcag gtggcgttta ataagctgaa caaattcgcg ctcgcgcctt tcaaagcaga
     5161 gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt
     5221 tagctatcag gataacgcgt tttccgttgg tccaattaat ctcaccatta aacgtggcga
     5281 gctgctgttt ctgattggtg gcaacggtag tggaaaatcg acgctggcga tgttgctgac
     5341 gggcttgtac cagccacaaa atggcgaaat cttgctggat ggcaaacctg tcagcggcga
     5401 acaaccggaa gattatcgaa aactgttttc ggcagtgttt accgatgtct ggctgtttga
     5461 tcaactgctg gggccggagg ggcaacccgc taacccgcaa ctggttgaga agtggctggc
     5521 gcagctgaaa atggctcata agcttgagtt aagcaacggg cgtattgtta acctgaagtt
     5581 atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg ctggcagaag aacgcgatat
     5641 tatcctgctg gatgaatggg cggcggatca ggatccacac ttccgtcgtg agttttatca
     5701 ggtgctgctg ccgctgatgc aggagatggg taaaactatt ttcgccatca gtcatgatga
     5761 tcattacttt atccacgccg accgcctgct ggaaatgcgc aacgggcaac ttagcgagct
     5821 gacaggcgaa gagcgcgatg ctgcttcgcg tgatgccgtt gcccggacag cataatatta
     5881 cgacgctgac ctggcggact gctgccgcca ggtcaatatt ctaaaaatac cttcgtttct
     5941 aataacccta taattaatca acgaaattat aatgtttcta aaattagaat ataatttata
     6001 aacattattt aaatgttgtt acttaagtgt taccgttgat gccgcgcaaa tctcacctgc
     6061 aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa agtgactgcc atgctcttct
     6121 cgatggccgt ggggcttaat gccgtttcga tggcggcaaa agcgaaagcg tccgaggagc
     6181 aggaaactga tgtactgttg attggcggcg gcattatgag cgccacgttg gggacctatt
     6241 tacgcgagct ggagcctgaa tggtcgatga ccatggtgga gcgcctggaa ggtgtcgcgc
     6301 aggagagttc gaacggctgg aacaacgccg gaaccgggca ttctgcactg atggaactca
     6361 attacacccc gcaaaatgcc gacggcagta tcagtattga aaaagcagtc gccattaacg
     6421 aagcatttca gatttcccgc cagttctggg cgcaccaggt cgagcgtggt gtgctgcgta
     6481 ctccgcgttc atttatcaat accgttccgc atatgagctt tgtctggggc gaggataacg
     6541 ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag ctcgctgttt cgcggtatgc
     6601 gttactctga agatcacgcg cagatcaaag agtgggcacc gttagtgatg gaagggcgcg
     6661 atccgcaaca gaaagtagcg gcaacacgca cagaaattgg caccgatgtg aactatggcg
     6721 agatcacccg ccagttgatc gcctccttac agaagaaatc taacttctcg ctgcaactca
     6781 gcagcgaagt ccgcgcccta aagcgtaatg acgataacag ctggaccgtc accgttgccg
     6841 atctgaaaaa cggcactgca cagaacatcc gcgccaaatt tgtctttatc ggcgcgggcg
     6901 gtgcggcgct gaaactgtta caggaatcgg ggattccgga ggcgaaagac tacgctggtt
     6961 tcccggtggg cggacagttc cttgtttcgg aaaacccgga cgtggttaat caccatctgg
     7021 cgaaggttta cggtaaagca tccgttggcg caccaccgat gtcggttccg catatcgata
     7081 cccgtgttct tgacggtaaa cgcgtagtgc tgtttgggcc atttgccacc ttctcaacca
     7141 aattcctcaa aaacggttca ttatgggatc tgatgagttc caccaccacc tctaacgtga
     7201 tgccgatgat gcacgttggg ctggataatt tcgatctggt gaaatatctg gtgagtcagg
     7261 tgatgttgag tgaagaggat cgttttgaag tgttgaaaga gtactatccg caggcgaaaa
     7321 aagaggactg gcgtttgtgg caagcggggc agcgcgtgca gattatcaag cgtgatgccg
     7381 ataaaggtgg cgtactgcgt ctcggtactg aagtcgtcag tgaccagcaa gggaccattg
     7441 ccgcgctcct gggggcatcg ccaggggcgt caaccgccgc gccgattatg ttgaatctgc
     7501 tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg gcaggctacg ttgaaagcga
     7561 tcgttccgtc ttatggacgc aagctgaacg gtgatgtagc ggcaacagaa cgcgagttgc
     7621 agtacaccag cgaagtgctg ggtctgaaat acgacaagcc gcaggcagca gatagtacgc
     7681 cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt ggcggatatt gcgttgtaat
     7741 gatgtgccac atccggcatg gtttgccgga tgtggcgtat gctgataaga cgcttcagcg
     7801 tcgcatcagg caaccggctc gggcgttaga tgtcggatgc ggcgtaaccg ccttattcgg
     7861 cctacaggtt gcagcacttc aaagcagttt agcgaacaac cgcgttgtca attttctctt
     7921 ccgccttcca gacacggtac ttcacatcca cattgtctgg cgtatacacc acgatcggca
     7981 gcttgctgtt gtaacgcagc attccagcat cgcccagata cgcggtgaca aatttcttct
     8041 ctttcttgcc atccgggcag gccatcatcg tggaaaccgg cgagctaact ttatcaaaga
     8101 cataataatc atagccccag ccttccagcg ttttgctttc cagcttcccg ccgagacgat
     8161 gcaaattgca gtcgacttcc agcgtctgac cgattaacag ttctactttc agggtagatt
     8221 catcttcttg cggagttaac tgaatcacct gacgcttcat gcctttttca gcttgtggat
     8281 aaggcgcgat tttttccagt ggctggacgc tctctgccgc ccaggcggaa gtggtggcga
     8341 aagcggcaaa caatactgca ggtagaatgg tcttcatttt tttgctttcc ttcttcattt
     8401 ataggttttc accgatgatg tcgctgaagg ttaacatatt catcagattt attaatttgc
     8461 tttatgaata acttaaagaa cattc
//
LOCUS       DACADA010000180         8476 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.182, whole genome shotgun sequence.
ACCESSION   DACADA010000180 DACADA010000000
VERSION     DACADA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.182"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(131..1102)
                     /locus_tag="ISB17_004127"
     CDS             complement(131..1102)
                     /locus_tag="ISB17_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAP1982000.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1102..2268)
                     /gene="sopA"
                     /locus_tag="ISB17_004128"
     CDS             complement(1102..2268)
                     /gene="sopA"
                     /locus_tag="ISB17_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAP1982001.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2856..3611)
                     /gene="repE"
                     /locus_tag="ISB17_004129"
     CDS             complement(2856..3611)
                     /gene="repE"
                     /locus_tag="ISB17_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HAP1982002.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4385..5191)
                     /locus_tag="ISB17_004130"
     CDS             complement(4385..5191)
                     /locus_tag="ISB17_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP1982003.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(5192..5497)
                     /gene="ccdB"
                     /locus_tag="ISB17_004131"
     CDS             complement(5192..5497)
                     /gene="ccdB"
                     /locus_tag="ISB17_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAP1982004.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(5499..5717)
                     /gene="ccdA"
                     /locus_tag="ISB17_004132"
     CDS             complement(5499..5717)
                     /gene="ccdA"
                     /locus_tag="ISB17_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAP1982005.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            6425..7420
                     /locus_tag="ISB17_004133"
     CDS             6425..7420
                     /locus_tag="ISB17_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP1982006.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            7424..8356
                     /locus_tag="ISB17_004134"
     CDS             7424..8356
                     /locus_tag="ISB17_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982007.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
BASE COUNT         2322 a         2071 c         1931 g         2152 t
ORIGIN      
        1 ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata ttcaggccag
       61 ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg tagactaaaa
      121 cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca attttctcta
      181 tacactcagt tggaacacga gacctgtcca ggttaagcac cattttatcg cccttataca
      241 atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt gatgcagatg
      301 acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat atcaccccag
      361 cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct ttatctgtaa
      421 aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga gaaaaaagag
      481 caacaactga tttaggcaat ttggctgtgt tgatacagcg ggtaataatc ttacgtgaaa
      541 tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc aatcggcttg
      601 cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc aatctggata
      661 atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca ctttcggtaa
      721 gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca gatactcttc
      781 gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga tcatccagtg
      841 agtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga gaggtcttct
      901 caacactatc accccggagc acttcaagag taaacttcac atcccgacca catacaggca
      961 aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa tccaccatcg
     1021 gggcagctgg tgtcgataac gaagtatctc caaccggttg agtattgagc gtatgttttg
     1081 gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca gacgatcgaa
     1141 aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac cagttgaaga
     1201 gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt taccaacttc
     1261 atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc gaatttgctc
     1321 ctccatccac ggggactgag agccattact attgctgtat ttggtaagca aaatacgtac
     1381 atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca tatcgaaaaa
     1441 ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca gcacatcagc
     1501 agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa taactatgac
     1561 atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg gatcggtggg
     1621 cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca gagccagaca
     1681 ggaaggaata atgtcaagcc caggccagca agtgggcttt attgcataag tgacatcgtc
     1741 cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa gatctggtac
     1801 ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga gcaaaacacg
     1861 tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt tgtaaacgcc
     1921 acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac gtcgcaatcg
     1981 cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa cacgttgctc
     2041 aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct cggcatctct
     2101 gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc agcgccgggt
     2161 tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag ccttcgtcat
     2221 ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc tgaacatcct
     2281 ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag caacaacaaa
     2341 atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac tacaaaagga
     2401 gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc agccgtgtaa
     2461 ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc agatagctct
     2521 tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga ggtacacacg
     2581 cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg atgacgaact
     2641 aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact gtgacaaact
     2701 gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa atccatgcag
     2761 gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac aaatgtgacg
     2821 aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat ggaagtgata
     2881 tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc aatgtatgag
     2941 aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa gcggcggcgg
     3001 aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat ccagtcgatt
     3061 ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga ttcgtataaa
     3121 cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg cgtaaaccgg
     3181 ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact gtaaagccct
     3241 ctggatggac tgtgcgcacg tttgataaac cagggaaaag attcatagcc tttttcatcg
     3301 ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact cttcaatgcc
     3361 tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc atatttagca
     3421 acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt tctgatctgg
     3481 tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc cgcctcagtg
     3541 aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt gataaccgct
     3601 gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc acactaaata
     3661 aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg agggcaattt
     3721 gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt gatttgtcac
     3781 actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa aggcctacaa
     3841 ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa atatccccgt
     3901 ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc agaatatata
     3961 agtgctgttc cctggtgctt cctcgctcac tcgagggctt cgccctgtcg ctcgactgcg
     4021 gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc attaggttgt
     4081 tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccacacga agatttctat
     4141 tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca tgacagaaca
     4201 ctacttccta taaacgctac acaggctcct gagattaata atgcggatct ctacgataat
     4261 gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc ttctctgttt
     4321 aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc caaggcattt
     4381 attctcagga taattgtttc agcatcgcaa ccgcatcaga ctccggcatc gcaaactgca
     4441 cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc gttgaactga
     4501 tggacttatg tcccatcagg ctttgcagaa ctttcagcgg tataccggca tacagcatgt
     4561 gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg aacagagaac gtcacaccgt
     4621 cagcagcagc ggcggcaacc gcctccccaa tccaggtcct gaccgttctg tccgtcactt
     4681 cccagatccg cgctttctct gtccttcctg tgcgacggtt acgccgctcc atgggtattt
     4741 tcagtgttgc caccatcgtc tgcagctggc tgacgtacca ggagtcagag agcggaacca
     4801 gccggtgagt ctgctgaccg gcgggcattc tccccgccgt cctggcagct ttttcggtcc
     4861 gttgtttcag ggtcgcaagc tgcacaaacg gatacggagg cgcaagcgaa aaatcccccc
     4921 gcgtcagcgc cagtgcttca ttaatgcgtg ctccggtgtt ccacagtgtg gccagcagca
     4981 tcttgcggtg cagatccggg acgtaatgga gcagggcact cacttccgga gccagcagat
     5041 attttggcag ttcatcatgg accatcgaca tctggcgaag tgccagagct gccggataat
     5101 caatggcaac aggcagcgat gcaggctgcc cggcagaata cactgccggt accatgactg
     5161 cagactggct gtgtataacg gagcctgaca tttatattcc ccagaacatc aggttaatgg
     5221 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
     5281 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
     5341 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca
     5401 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
     5461 ggctctctct tttataggtg taaaccttaa actgcatttc accagtccct gttctcgtca
     5521 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
     5581 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
     5641 ccggagatat tgacatcata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
     5701 gtaatacgct gcttcatagc acacctcttt ttgacatact tcgggtatac atatcagtat
     5761 atattcttat gccgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta
     5821 agccttatgt attttacctt tcgttatgtt aaccaataaa aattaaaatc tgacttataa
     5881 aaacaaagcg taattaccgc attcccgttt cgtatggatc cgactttatt tgaataaacg
     5941 actttccgga agtgtacaac gctgagaaaa tcatcatatc gctgttttcc gcccctgcgc
     6001 ggcgcatcac agtccagaga tacaacacaa catatagtgc cattggcaac tgtatgaggc
     6061 actacatgct gtgctgacgg gatgtaacgc tgtgcggagg gagaggaaga tccgtaactt
     6121 acttttttcg gatctctgga gaggggaaga aggatttgtg ctgatgatgc aatactaatg
     6181 atatattttg aacttaattg cattattgct tatctggcat taataactga aatactttgc
     6241 ataattatca atgaaagaac gaggctcata tttcccatgt aaattatatg cccttgcaga
     6301 gagcgagacg gtagtgaact tgcggcacta ataataaact gtaccgactc ttcccgggca
     6361 ctggaatgtt cagatgtacg acagagcccc gcgccttttg caataaataa ttaaaggact
     6421 gaacatggcg accacagtaa tagctgcctt taacgaattt atgaaagata ccgtgaatct
     6481 caaaaaggca gataccgatg acgcgcgtgc aagtcgcgac tggcttatcg gtaagatgaa
     6541 tgattttgag aaggacgata aatttccggt gagttttcca gcgatccata ttgcctttgg
     6601 ctcctttgcc agaagaacca aaatccgtcc gcttgatgat atcgatctga tgtttggctt
     6661 aaccgggcaa ggtgccacct ataccatcct gagtgaccgg ataactgtaa cctcctccgg
     6721 agaggggtca cgtctgcaca gttaccgcca ttccggagct gacaccgtct gctccgtcag
     6781 aattctgaat gcctttaaaa accgcctgca ggacattgcc cagtacgccc aggctgatat
     6841 caggcgtaac caggaagctg tcacgctgaa actggtcagc aaggactgga attttgacat
     6901 cgtcccctgc tttattacca gcgaagatgc ctttggacgg acttactacc tgatccccga
     6961 tggcaacggc cactggaagt ttaccgaccc ccgtaaagac agggacagag tcactactat
     7021 caatgtgcag aataacggca atgtgctcaa tgtcatcaga gcagtcaaat actggcagcg
     7081 gcgaccgacg atgccatcca tgagttccta cctgctggaa acgctgattc tggactatta
     7141 cgctggcagg acatcctgct catcgtttgt tgacatggag ctggaagcgc ttttccgcca
     7201 ccttggtcaa tccgtgcggt actctgtcaa cgatcccaaa ggcatacagg gtgatatcaa
     7261 ctcactttca gccgaggcac gtaaggcgat atcggacaga tgttatctcg atgcgcaaaa
     7321 ggtatcggag gcgagatggt ttgaaaataa taaagagtat gaaaaatcca tcaataaatg
     7381 gcgcgatgtg ttcgggccat tctttcctgt ttacgggtga atcatgaatg ccatttacac
     7441 aaaccagtcg accagagaaa acatggattt actgtatgcg caggcccgtg tctacgacag
     7501 ggtaaaaaac tggaacagac tgaattttct cttcagcatc atcgtaccgc tcctgctttc
     7561 tttcgttacc gtatacaacc ggagccggga atttgtcgat tcagagttgt tgtcttcgct
     7621 gttggggtta tatggactcc tggtgcttac ctttaacatt gttatcagtg gacacatctc
     7681 ggctctgcgc aggaaggcag cctcaatcca ggaaatgtac gactgccggg tacttggcat
     7741 ccggcgtaat gaactgaagg tggaggagat cccacgggat gacatcatcc gggaagctgc
     7801 atattttaga gacagcccgg aaaaagcacg aaagcgattt ggcgaagagg ggtggtacgt
     7861 cagtaaggtg tatgacgcgc cgcaggccgt aatggcgctc ctttgccacg gaaaaaatct
     7921 cggctgggac aaatcgctca gggaggttct gcacgtattt tatctttccg cttttattgt
     7981 ctcgcctgtc gcgatgcttg tttacggcat cgtcatgaaa tccgggctga atgagatcct
     8041 cttctatgtg gtgttcacac tgccggtcat ccgctatttc ttgcttcagt ttctggataa
     8101 tcgcagcagt atgaaaagaa gcgagaagct gaaaaagtac gttgagaagg aattatccgg
     8161 cataagggtg tccggaaggg cagaagaaga gcaactggct tatacgctcc ggaatatcca
     8221 agatgagatg tttacctacc gggcctcatg tccgcccgta cccaacggta ttcagctgat
     8281 aatgaagccc aaaaatgaac gaatttatgt tgattacttt gaaacaaatc tgaaggagct
     8341 gcaccttcag aagtaatccc tccttagctc cggtgtagga tttacgtatg ccggagcaca
     8401 tccgataaac aattcttggt tgcctgagtg tcagcgtaaa cccatagtca ccttcgcctc
     8461 agacggttag taacac
//
LOCUS       DACADA010000181         8246 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.183, whole genome shotgun sequence.
ACCESSION   DACADA010000181 DACADA010000000
VERSION     DACADA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.183"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..369
                     /gene="ecpR"
                     /locus_tag="ISB17_004135"
                     /gene_synonym="matA"
     CDS             <1..369
                     /gene="ecpR"
                     /locus_tag="ISB17_004135"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HAP1982008.1"
                     /translation="INRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            444..1031
                     /gene="ecpA"
                     /locus_tag="ISB17_004136"
                     /gene_synonym="matB"
     CDS             444..1031
                     /gene="ecpA"
                     /locus_tag="ISB17_004136"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HAP1982009.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1088..1756
                     /locus_tag="ISB17_004137"
     CDS             1088..1756
                     /locus_tag="ISB17_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HAP1982010.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1782..4307
                     /locus_tag="ISB17_004138"
     CDS             1782..4307
                     /locus_tag="ISB17_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982011.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4297..5940
                     /gene="ecpD"
                     /locus_tag="ISB17_004139"
     CDS             4297..5940
                     /gene="ecpD"
                     /locus_tag="ISB17_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HAP1982012.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5909..6619
                     /locus_tag="ISB17_004140"
     CDS             5909..6619
                     /locus_tag="ISB17_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HAP1982013.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6926..7255
                     /locus_tag="ISB17_004141"
     CDS             6926..7255
                     /locus_tag="ISB17_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP1982014.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7267..7430)
                     /locus_tag="ISB17_004142"
                     /pseudo
     CDS             complement(7267..7430)
                     /locus_tag="ISB17_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7504..8118)
                     /locus_tag="ISB17_004143"
     CDS             complement(7504..8118)
                     /locus_tag="ISB17_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HAP1982015.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2174 a         2125 c         2103 g         1844 t
ORIGIN      
        1 ataaaccgtg ataatgagtg gttatccgcg gtaaagggga aacaggtcgt attgattgcg
       61 gccagaaagt cagaagcctt agcaaattat tggtattaca acagcaatat taggggcgtg
      121 gtatacgctg gactgagtcg tgatattaga aaagaactgg cctatgtgat taatggcagg
      181 ttcctgagaa aagatattaa gaaagataaa atcactgacc gggaaatgga aattatccgc
      241 atgacggctc agggaatgct gcctaaatcg attgccagaa ttgaaaattg tagtgtgaag
      301 acagtgtata cccatcggcg taatgcagag gccaagctgt actcaaaatt atataagttg
      361 gttcagtaaa ctccaggcaa gttagtttta aaaaatgact caccgggact tcatgtcctc
      421 aattcaactc gggaagaaaa gcaatgaaaa aaaaggttct ggcaatagct ctggtaacgg
      481 tgtttaccgg tacaggtgtg gcgcaggctg ctgacgtaac agctcaggct gtagcgacct
      541 ggtcagcaac agccaaaaaa gacaccacca gtaagctggt tgtgacgcca ctcggtagcc
      601 tggcgttcca gtatgccgaa ggcattaaag gttttaactc acaaaaaggt ctatttgacg
      661 tggctatcga gggtgactca acggctaccg cctttaaact gacctcacgt cttatcacca
      721 acaccttaac ccagttggat acctcaggtt ccacactgaa tgtgggcgtg gattataacg
      781 gcgcggcagt cgaaaaaact ggcgataccg tgatgatcga taccgccaac ggcgtactgg
      841 gcggcaacct tagcccactg gctaacggtt acaatgccag caatcgtacc actgcacagg
      901 atggtttcac tttctccatc atcagcggca ccaccaatgg taccaccgca gtaaccgatt
      961 acagcactct accggaaggc atctggagcg gcgacgttag cgtacagttc gacgctacct
     1021 ggaccagtta atctctctga tgtaccagca ggggtacccc cctgctttat tcctggacgg
     1081 aattgttatg aaaaagcacc ttctgcctct cgctctgctg ttttccggaa tatcaccggc
     1141 ccaggcgctg gatgtcggcg atatatcatc gtttatgaac agtgacagca gcacgctgag
     1201 caaaacgatc caaaacagta ccgacagtgg ccgcctcatc aatatccgtc tcgaacggct
     1261 ctcttcaccg cttgacgacg ggcaggttat cgcaatggac aagccggatg agttgctact
     1321 cactcccgcc agcttgctgc tacccgccca agccagcgaa gtgatccgct tcttctataa
     1381 gggaccagca gatgaaaaag agcgctacta ccgcattgtc tggtttgatc aggccctcag
     1441 tgatgcgcag cgcgataatg ccaaccgcag cgctgtggcc actgcctccg cccgcatcgg
     1501 caccatcctg gtcgtcgcac cccgccaggc aaactaccac tttcagtacg ccaacggctc
     1561 cctgacaaat acaggaaatg cgacgctgcg gatcctcgcc tacggcccct gcctgaaagc
     1621 cgccaatggt aaggagtgta aagagaatta ctacctgatg ccgggcaagt cgcgtcgttt
     1681 tacccgcgtg gacacggcgg ataacaaagg acgggttgca ctttggcagg gtgataagtt
     1741 cattcccgtg aaatagatag ctgtgcagat ggataacgac aatgccttta cgacggttct
     1801 ccccaggact gaaagcccag tttgccttcg gcatggtctt tttgttcgtt cagcccgatg
     1861 ccagcgctgc tgacataagt gcgcagcaaa taggtggggt gattattccg caggccttca
     1921 gtcaggcgct ccaggacggc atgagcgtcc cgctttatat tcatctcgcc ggtagccagg
     1981 gtcgccagga cgatcagcga atcggcagcg cttttatctg gttggatgat ggacagttac
     2041 gcatccgaaa aatacagctg gaagagagtg aagataacgc cagtgtcagc gaacaaactc
     2101 gacagcagct gatgactctg gccaacgccc cgttcaatga ggcccttacc atccccctga
     2161 ctgacaacgc gcagttggat ctcagcttgc gccaactgct gctgcagctg gtggtcaagc
     2221 gcgaagcgct gggcaccgta ctacgctcac gtagcgaaga catcgggcag tccagtgtta
     2281 acaccctcag cagtaatctg agctataact tcggcgtcta taacaaccag ttgcgtaacg
     2341 gcgggagcaa cacctccagc tatctgtcgc tgaataacgt tactgcgctg cgcgaacatc
     2401 acgtggtgct cgacggctca ctgtacggga ttggtagcgg tcaacaggac agtgaattat
     2461 ataaagcgat gtatgaacgc gattttgctg gtcaccgatt tgccggtgga atgctcgaca
     2521 cctggaactt gcagtcctta gggccgatga ccgccatttc agcagggaag atttacggcc
     2581 tttcctgggg aaaccaggcc agctccacca tcttcgacag cagccagtcg gccacgccag
     2641 tgatcgcctt tttaccggcg gcgggcgaag tacatctcac ccgtgatggg cggctactaa
     2701 gcgttcagaa cttcaccatg ggcaatcatg aagtggatac ccggggtcta ccgtacggta
     2761 tttacgatgt ggaagttgag gtaatcgtta acggtcgcgt gatcagcaaa cgcacccagc
     2821 gggtcaataa gctgtttagc cgggggcgtg gcgtcggtgc accgctggcg tggcagatat
     2881 ggggcggtag ctttcatatg gatcgctggt cggaaaacgg gaaaaagacg cgaccagcca
     2941 aagagagttg gctggcaggt gcctcgacct ccggctcact gagtacgttt agctgggcgg
     3001 caacgggata tggatacgat aatcaggcgg tgggtgaaac ccgtctgacg cttccgcttg
     3061 ggggggcgat caacgttaac ttgcaaaaca tgctggccag tgacagctca tggagcaaca
     3121 tcgccagcat cagcgccact ctacctggag gcttcagttc gctgtgggtt aaccaggaaa
     3181 aaacccgcat tggcaatcaa ttgcgacgta gcgatgccga caaccgtgca atcggcggca
     3241 cactcaacct gaactcactg tggtcgaagc tgggtacgtt cagcatcagc tacaatgatg
     3301 accgccgcta caacagccac tattacacgg cagattacta tcaaagtgtc tacagcggta
     3361 cctttggttc gcttggcctg cgggccggta ttcagcgcta taacaacggc gacagcagcg
     3421 ccaatacagg gaaatatatc gctctcgatc tctcgctacc actgggcaac tggtttagcg
     3481 cagggatgac ccatcaaaac ggctacacca tggcaaacct gtcagcacgc aaacagtttg
     3541 atgaaggaac cattcgcact gttggtgcca atctgtcacg agccatctcc ggcgataccg
     3601 gtgatgacaa aaccctcagc ggtggggcgt atgcacagtt cgacgctcgt tacgccagcg
     3661 gaacgctgaa cgtcaatagc gcggcggacg gctacatcaa tactaatttg accgccaacg
     3721 gcagcgtcgg ctggcagggt aaaaacatcg ctgccagcgg gcggaccgat ggcaacgctg
     3781 gggtgatatt cgacactggc ctggagaacg atggtcagat cagcgccaaa atcaacgggc
     3841 ggattttccc gcttaacggc aagcgtaact atctcccgct ctctccctat ggaagatatg
     3901 aggtggagtt gcagaacagc aaaaactcac tcgacagtta cgatatcgtc agcggtcgca
     3961 aaagtcatct gactctctat ccaggcaatg tcgctgtcat tgagccagag gtgaagcaga
     4021 tggttaccgt ctccggtcgt atccgtgcgg aagacggcac actgctggct aacgcacgga
     4081 ttaacaacca tatcggccga acccgaaccg atgaaaacgg cgagtttgtc atggacgtgg
     4141 ataagaaata ccccactatc gattttcgct acagtggcaa taaaacctgc gaagtggcac
     4201 tggaactcaa ccaggcgcgc ggtgccgtct gggtcggtga tgtggtctgc agcggcctct
     4261 catcgtgggc ggcggtgacg cagacaggag aagagaatga gagttaacct actgatagcg
     4321 atgataatct ttgcgctaat ctggcctgca accgcgctca gagcggcagt gagcaaaaca
     4381 acctgggcgg atgctccggc acgcgagttt gtgtttgtcg aaaacaactc agacgacaac
     4441 tttttcgtca ctcctggcgg ggcgctggat ccgcgcctga ccggtgccaa ccgctggacc
     4501 ggtttaaaat acaatggttc aggaaccatc tatcagcaaa gcctcggcta cattgataac
     4561 ggttacaaca ccggccttta taccaactgg aagtttgata tgtggctgga aaattcacca
     4621 gtttcatctc ctttaactgg cttgcgctgc atcaactggt acgcagggtg taatatgacc
     4681 accagtctta tcctgccgca aaccaccgac gccagtggat tttatggcgc gacggtgacc
     4741 agcggcggcg cgaagtggat gcacggcatg ttgtcagacg cgttttacca gtatctgcaa
     4801 caaatgcccg tcggcagcag ctttacaatg accatcaatg cctgccagac ctctgtgaac
     4861 tatgacgcca gcagcggcgc acgctgtaag gatcaggcct ccggcaactg gtatgttcgc
     4921 aacgtcaccc atacgaaagc agcaaatctg cggttgataa atacccactc gctggcggaa
     4981 gtatttatca acagcgacgg agtaccgact ctgggcgaag ggaacgccga ctgccggacg
     5041 caaaccatcg gcagccgttc aggattaagt tgtaagatgg ttaactatac cctgcaaaca
     5101 aacggactca gcaacacctc aatccatata ttcccggcga tcgccaactc gtcgttagcc
     5161 tcggccgtcg gggcgtacga tatgcagttc agtctgaatg gcagttcatg gaaaccggtg
     5221 agcaatactg cctattacta caccttcaac gagatgaaga gcgcagactc gatctatgtg
     5281 ttcttctcga gcaacttctt taagcagatg gtgaacctcg ggatcagcga tatcaacacc
     5341 aaagatctgt tcaactttcg ctttcagaac accacatcac cggagtctgg ctggtatgaa
     5401 ttttctacct ccaacacgct gattatcaaa ccccgtgatt tcagcatcag tattatctcc
     5461 gatgaatata ctcagacacc gtcgcgggag ggatatgtcg gcagcggcga gtcggcactc
     5521 gatttcggct atatcgtaac caccagcggt aaaacagctg ccgacgaagt gctgatcaag
     5581 gtgaccggac ccgcgcaggt gattggcggg cgctcctatt gtgtcttcag ctccgatgac
     5641 ggtaaggcga aagtaccgtt cccggcgacg ctttccttta ttacccgcaa cggagctaca
     5701 aaaacctacg atgccgggtg cgatgatagc tggcgggata tgaccgatgc gctgtggttg
     5761 accacaccgt ggactgatat ctctggcgaa gtggggcaga tggataagac cacagtcaaa
     5821 ttttcgattc caatggataa cgccatttct ctgcgtacgg tagatgataa cggctggttt
     5881 ggcgaagtca gcgcttcagg agagattcat gttcaggcga cgtggcgtaa cattaactaa
     5941 ggccctgctg acagtggtct gtatgctggc ggcacctttg acacaggcga tttcggtcgg
     6001 caatctgact ttttcactgc cgtccgagac tgactttgtc agcaaacgtg tcgtgaataa
     6061 caacaaaagc gcacggatat accgtattgc catcagtgct attgatagcc cgggcagcag
     6121 tgaattgcgc acccgaccgg tggatggtga actacttttc gccccccgcc agctggcgtt
     6181 gcaggctggt gagagcgagt attttaaatt ttactatcat ggcccacggg ataaccgcga
     6241 gcgctactac cgggtctcat ttcgcgaggt ccccactcgt aacctgacaa aacgcagccc
     6301 aagcggcggt gaggtcagca cggagccggt ggtggtgatg gataccattc tggtagtacg
     6361 accacgtcag gttcagttta aatggtcctt cgatcaggtg acaggaacgg taagtaacac
     6421 cggcaacacg tggtttaagc tactgattaa accaggatgt gattcgaccg aagaggaagg
     6481 cgatgcctgg tatctacgtc cgggagacgt ggttcatcag cctgagttac gtcagccggg
     6541 gaatcattat ctggtctata acgacaaatt cattaagatt agcgattcct gtccggctaa
     6601 gcccccttcg gcggactaag acttatccat cggacgaggg aagaattcca cctcagagac
     6661 tcaaaaatcg tttaaatgat gtggaagcga tcgtcaatgt caccgatgag ctttctcata
     6721 tgcacaccac agtgttcagg agacacggaa aggtaccagg cggcctgcta acgagtggtt
     6781 tcttcactaa ttaggacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat
     6841 cgaaagtaca ccagccaacc gcgatacgtc cctgcatacg acgtaccgca gttatcttta
     6901 cgatttatag ctgtactggt gaattatgag caatttgaat ccatgcatga cgtgtggtgc
     6961 ctgttgtgca tttttccgcg tctcttttta ctgggccgaa gccgacgatg ctggcggtac
     7021 cattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtactaa
     7081 tcagaaaaat ccccgatgta ttgcccttgc aggaacaccg ggcaaaaatg cctgctgcac
     7141 gatatatgaa aatcgatcgt ccacatgcag agaattcgcc atgtctggtg agaacggaga
     7201 agtcaatgag gcttgcaatc gtgcaagagc taaatacggg ctaacaccgt tataaatata
     7261 caataattaa ttgcattgcc ccgccagctg taatagcggg gcttcgtttt tcagggacaa
     7321 caaaacctga aacttcttcc attttccagc aacaatgcct cttcttctgt agcgtcctcc
     7381 ggatctacat catcctgaag tagcgcgata tgatcactga ctgtccgcat cagaatatga
     7441 aaagcaacgt ttttaaccta taaaaatggc gctatatttg cgccattttt atcattcaaa
     7501 gcattatcta tttgagccta aagggatctc aggatctggc tcatgggtaa ttctgtttcg
     7561 taaatctctg cgaataattt caatagacca gaaccagact aaatgtccaa aaatttcaga
     7621 gacattctca taccacggaa gatcaaagag aggtggcgta agtcccatga gagggaatga
     7681 aatcatatga acaaaaagtt gcgctaaagc acctgccagt aagccctgcc aaagtttaat
     7741 tttcggaaat acttcggcga ccacacagta accaacggca aaaactatcg aaaagattat
     7801 gtgcgttaca ccaacccagt taaagacatg cccggcgaag gtataaacag ccgaatttgg
     7861 atctgtcagt cccaaccaat cccgaagaaa aatgtacgga ggattgagaa agttacggga
     7921 gcaatcaatt tggcctgcag ccctgattaa tgattccgga ccacacgctg cattaaacat
     7981 atcaaccgga ctacgtggtg gcaatggaac ttctgccccc cacttaacaa atgcggaaac
     8041 aacgccagca atcagcccaa tgaatgcagc aagaccataa cgtctgcggt ttggtggagt
     8101 ttgttcaaat atattcatat ctaccctgct tgtaccatta tgttatacac ctctttagga
     8161 gtattcataa aacaaggcaa atgtaaagaa ctgtattgtt ttgtataacc acatggttac
     8221 ctaatcgcca attaatataa gctcca
//
LOCUS       DACADA010000182         8221 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.184, whole genome shotgun sequence.
ACCESSION   DACADA010000182 DACADA010000000
VERSION     DACADA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.184"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            14..868
                     /locus_tag="ISB17_004144"
     CDS             14..868
                     /locus_tag="ISB17_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982016.1"
                     /translation="MEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKL
                     EIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSN
                     HNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGER
                     MTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCY
                     DNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFR
                     EKYHQMAA"
     gene            1023..3020
                     /locus_tag="ISB17_004145"
     CDS             1023..3020
                     /locus_tag="ISB17_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAP1982017.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATGLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            3083..4360
                     /locus_tag="ISB17_004146"
     CDS             3083..4360
                     /locus_tag="ISB17_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAP1982018.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATHADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            4608..5264
                     /locus_tag="ISB17_004147"
     CDS             4608..5264
                     /locus_tag="ISB17_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP1982019.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYFKSKEDYAQTVLNAYDSFFEHKLKKHLHESSCTPMVRLENFI
                     NDACEGIKKYNFTRGCLVGNMMQESPGLPQSFIKVLQNILESWQALVAACLSDALSSG
                     EISSNMNNTQLAAIFWSGWEGAVMRSKLYCSTEPVYDFWSYFKTSVRYQSSQEATTGQ
                     "
     gene            complement(5445..5552)
                     /locus_tag="ISB17_004148"
     CDS             complement(5445..5552)
                     /locus_tag="ISB17_004148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP1982020.1"
                     /translation="MGNMAPFILRGVTLKGIDSVMFAKEKRAAIWQQAV"
     gene            6678..6776
                     /locus_tag="ISB17_004149"
     CDS             6678..6776
                     /locus_tag="ISB17_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAP1982021.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
     gene            complement(6778..7560)
                     /locus_tag="ISB17_004150"
     CDS             complement(6778..7560)
                     /locus_tag="ISB17_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1982022.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            7866..>8221
                     /locus_tag="ISB17_004151"
     CDS             7866..>8221
                     /locus_tag="ISB17_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1982023.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSP"
BASE COUNT         2154 a         1838 c         2015 g         2214 t
ORIGIN      
        1 aaaatacgcg ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt
       61 atttggtgta tccaggagcg gttattacaa ctgggtacag catgaaccct cagacagaaa
      121 acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac
      181 atatggaacc cggcggctcc agacggagct ggcagagaat ggcatcatcg ttggtcgtga
      241 ccgactggca cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag
      301 agcgactacg aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt
      361 cgctcctaca gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga
      421 gggatggttg tacctcgctg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc
      481 catgggagag cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag
      541 ccagcgccca cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata
      601 cgattaccgg gtcatacagg agcagtttgg tctgaaaaca tcaatgtcgc gtaaaggtaa
      661 ctgttacgac aacgctccga tggaaagctt ctggggaacg ctgaaaaatg agagcctgag
      721 ccactatcgt tttaataacc gggatgaagc catctcagta atacgggaat acattgagat
      781 tttctacaat cgtcagcgtc gtcactctcg tctggggaat atctccccgg cagccttcag
      841 ggaaaaatat catcagatgg ctgcttaaaa aaaagaacaa atggtagtgt ccgctattgc
      901 cagtacacct caacattcca ccatgcattc cgattaacgc cgcatagcca gttgaacttt
      961 gctactttgt gagaggtagt accttctatc cagtgcgaat ttaattaatg gaataaatga
     1021 ttatgagtga aaatgataca atcccaaaga agtctacaag tcagattaac aaagcggtat
     1081 tctttacatc tgctttgcta attttccttc ttgtcgcctt tgccgccgta ttcccggatg
     1141 tcgccgacaa aaattttaaa ctacttcagc aacaaatctt cacgaatgcc agctggttct
     1201 acatccttgc tgtggccctg attttactga gtgtcacgtt ccttggactc tcacgctacg
     1261 gtgatatcaa gctgggcccg gaccatgcgc agcctgattt cagctaccac tcctggtttg
     1321 cgatgctttt ttcggcaggg atggggatcg gcctgatgtt ctttggcgtt gccgaacctg
     1381 taatgcatta tctttcgcca cccgttggca ctccagaaac cgttgcggca gctaaggaag
     1441 caatgcgtct gacctttttc cactggggac tgcacgcatg ggcaatttat gccattgtgg
     1501 cgctgattct ggcgttcttc agttaccgtc acggtctgcc tttaactctg cgctccgcac
     1561 tctatcccat tattggcgat cgcatatacg gacctgtagg acatgcggtt gatattttcg
     1621 ctgttatagg cacggtcttt ggcgttgcga catcactggg ttacggtgtt ttgcaggtga
     1681 atgccggttt gaaccatctt ttcggggtgc ccatcaatga aacggtgcag gtaattctga
     1741 tcgtggtcat cacggggtta gcgacgattt cagtggtgtc cggtctggat aagggaatac
     1801 gtatcctgtc tgaactcaat ctgggtctgg ctttgttgct cctggcgctg gtcctgtgtc
     1861 tgggaccaac cgtgcttctg ctgaagtcat ttgtggaaaa tacgggcggt tatctttcgg
     1921 aactggtgag taaaacgttc aacctttacg cgtatgagcc caagtcgagc aactggctgg
     1981 ggggctggac attactgtac tggggatggt ggctttcatg gtcgccgttt gtggggatgt
     2041 tcatcgcacg ggtctcccgc gggcgaacca ttcgcgagtt tgtcaccggc gtgctgtttg
     2101 ttcccgcggg ttttacgcta atgtggatga cggtgtttgg taacagcgcg atctatctca
     2161 ttatgaacca gggggccaca ggcctcgcca atactgttca gcaggatgtg gcgctggccc
     2221 tgtttaattt cctggagcat ttcccgttct cttctgtgct gtcattcatt gcaatggcga
     2281 tggtcatcgt cttctttgta acgtctgctg attcgggggc aatggttgtg gatactctgg
     2341 catcaggtgg agtggcaaac acacccgtct ggcagcgaat attctgggcc tcgctcatgg
     2401 gcattgttgc aattgcgctt ctccttgccg gagggctaag tgcgctgcaa acggtgacaa
     2461 tagcgagtgc attgcccttc tcagtgatct tactaatatc tatatacgga cttttaaaag
     2521 ctttgcgccg ggatttgacc aagcgtgaaa gcctgagcat ggcgacaatt gctcctacgg
     2581 ctgcacgtaa cccaattcct tggcagagaa ggttacgcaa tatcgcgtat ctgccgaagc
     2641 gatctcttgt gaaacgtttt atggacgacg ttatccagcc cgccatgacg ctggttcagg
     2701 aggaactgaa caagcagggg acgataagcc acattagtga tgcagtcgac gatcgtattc
     2761 gtcttgaagt cgatttgggc aacgagctga attttatata tgaagtgagg cttcgcgggt
     2821 atatctcacc gaccttcgcg ctcgccgcaa tggataatga tgagcagcag actgaacaac
     2881 atcgatatta tcgcgctgag gtttatctca aagaaggcgg tcaaaattat gatgtgatgg
     2941 gctggaacca ggaacagctg attaatgaca tactggacca gtacgaaaaa cacctgcact
     3001 tcctgcacct ggttcgttaa tagcaacatg ccgtcctggg ggcggcaatt attatcccgg
     3061 ccgcaatatg agggaatgca gaatgatttc acgctggaaa tggatgctga agcagacaat
     3121 taaaaaacta tggttcaggg caacgttatt cgcaattgtc gcgataataa cggccctttt
     3181 atcaattctt tttaaatcaa tgatacctga gtcggtttcc gtgaaggttg gtgcggaagc
     3241 agtcgataac attctgaaca tactggcatc gagtatgctg gcagtgacca cattttcgct
     3301 gagtatcatg gtcacagcct acggttcagc cactactaat gtgactccca gagctacgcg
     3361 tttagttgtt gaagacgtca ccacacaaaa tgtactggcc accttcatcg gttcttttct
     3421 cttcagtctg gtagggatta ttgccctcaa tatgggagct tatggagaaa gggggagagt
     3481 cattttattc attgtcacac tggttgtcat tgccttaatc ctcatcacat tgcttcgctg
     3541 gatacagcat ttgacctctc tggggagggt tggtgagaca acggcacaag tagaacaggc
     3601 ggccatcgaa acatttattg cgagagcaag aaatccctgt ctcggcggat atccatggct
     3661 tgagaacaat gaacagccga aaggaacagt tgcagtttat ccgaagaaga ttggctatgt
     3721 tgaatatatt gatatggtga aacttagcaa gctgctgacc aatgatcccc gtcatgtata
     3781 cctcgtggcg cagccaggca gtttcataca tccgtccatg ccagttttgt acctgagtca
     3841 gggccaggag tcgtcaatca acaccgattt acttgagacg attattgtct cggatgcacg
     3901 ttcatttgct caggatcctc gattttgtct tagcgtcatg gccgaaatag cctgccgggc
     3961 cctttcccct gcagtgaacg atcctggaac cgccattgat gtcattggca gaggtgttcg
     4021 tatactttcc acttacgcgc agaataaatc tgatgaaata gaagtgaaat atccttcagt
     4081 acatgttgca ccacttcaga ataacgatct actggaagac tttttctcac ctgtcgcgcg
     4141 cgatggtgcc agtatgaggg agattcagat aagagtcctt aaaggactgt cgatgctgag
     4201 taaaggttgg cctgagatat ttgctgaagc cgcacatacc ctggcatttg aaacattaga
     4261 gcatgcaact catgctgacc atatagattc tgatagatat ctaataaaat caacttatta
     4321 taatttattt agtggcgaat attctaataa aaaaacatag ctgcagagca agcaattcgg
     4381 ttgttggcaa gtcaagggca aagcattcgc gtgagaatgg gcgtatgctt tgtgccaaaa
     4441 gcaaacgttg ctaattctgc actgttcagc gatggcgcag tagcgacagg cactgccact
     4501 gtccagtcgg caaaagcgaa cactttgcca ctgcggggat aaactgattg aagctctcgc
     4561 gcaaaatcac ttattctttt gcgttaataa aatgtaggag atgggtcatg acaggcgaaa
     4621 atcgtggccg gggaaggccg cgaaagacag aaagtaccta tgcagatact cgtaatgatc
     4681 tgatccgcag tggccttgaa ctgctgacgc agaatggttt tcttgcgacg ggagtcgatg
     4741 ccattgttaa aaatgccaat gtccctaaag gctcatttta ttactacttt aagagtaaag
     4801 aggattatgc tcagaccgtt ctgaacgcct atgacagttt cttcgagcat aaactcaaaa
     4861 aacatctaca tgaatcatcc tgcacaccaa tggtacgtct ggaaaatttc atcaatgatg
     4921 cctgtgaggg gattaaaaaa tacaatttta cccgcggctg cctggttgga aatatgatgc
     4981 aggagagtcc ggggcttccc caatccttca ttaaagtact acagaatatt cttgagagct
     5041 ggcaggcact tgttgcggcc tgcctgtccg acgcgttatc ttcaggcgaa ataagttcaa
     5101 atatgaataa tacacaactg gcggctattt tctggagtgg ctgggaaggt gcggtaatgc
     5161 gttccaaact atattgttct actgagccag tgtacgactt ctggagctat ttcaaaacct
     5221 cggtacgcta tcagtcttct caggaagcaa caactggcca gtaaaagggg ccgcagcccc
     5281 taaaacttat tatactaagc ctgagctcgg catccatcta attagctata ttgaatgact
     5341 gcacgaccgc gaattccgcc actcagaagt ttttcagcca cagtgatggc gtcactgagc
     5401 ggataaacgg tagtgagact atccattctg gactctggca aatactatac cgcctgttgc
     5461 cagattgctg ctcttttctc tttcgcgaac atgacgctat caatcccttt cagcgtgaca
     5521 ccacgcagaa taaacggagc catattgccc atcaaatcca tcccctgagc catgccgcag
     5581 gctgcaacaa tactgccata tcggaccccg gcgcaggcat ttctccagta acagggggtt
     5641 gtattgtatt gtattttatt gtgccggttc aggtgtgcgt ttcccggcgc gtctgcaccg
     5701 gcttaaccaa attcaacagg gataaaatag tgcaaataac cgaagtatta cttgcagtaa
     5761 tcgggggaag catccggggt tttattcaac aggctgtaga tcactgactg cgtctgttgg
     5821 ccgtccactt cacgcgtcat tcgaccagag taaatattga tgattactag attcgtgtat
     5881 tttatcaggc attttacttg cgaataaatc cccttgctat ccagcgaaac cacacaggta
     5941 gctattacca tatgtggtaa tacactagtt acaggaaaaa tatgcaagtt ccgcaatacg
     6001 ttgtgtgttg ctccttagcc aggatctttt cttccatgcg agatatgaca acaattttga
     6061 ggaaggatgt tcgtaactgg tagagttact acaacaagca tgggaggctc tgactctgac
     6121 agacattgta ctgtggaatg ttgttacagg ttggggcggc aaccgtgcca ggaaaccatg
     6181 agccgttcac ggcattaaag gccacacttc agtcgtttac attccctatg gtggcaggca
     6241 tcttccatta aggcgtcaga gcgttaccaa cgttatgttg tcatggctca tctttcatag
     6301 tcgatgctta tctggtggct gaagctagcc gcagataaag agtgtgaagt tgataccatg
     6361 gagtggagtg acgtgcaggc aggagattcg ctctatgcag ggttgtgaga tgaattcaaa
     6421 ccggctggca gcgccagtga tattcgaaga ttcgtccggt tgttacccag tatgcattaa
     6481 aaacccatac attatggaca atataacggc ttctacaaca aggaagtgcc tgtttaccat
     6541 ccgggtgtaa tgactcacct ttgtgggctt ttgcccgtcg cgctcttaac gttgaagaca
     6601 ttctctgcct gccgattcag gactgcgaca aaagccatat ctggctactg gtcaaggatg
     6661 accagcgtct ggagtaggtg ataagccaaa tagataagct ggaatatgtg gtgaaagtgc
     6721 aacgtaatca gtccaacccg acgatgttta acaagattgt ggtgtttttt cagtaagtca
     6781 ttcgtcttct gaatcaggtg taatgatctt tacagaacca ttctggcagt gcgtaacata
     6841 atctgccggt aacggacgat cgctaatcag aacgtcaaat gcagagatcg gtgcgaggct
     6901 ggcagaggcc acttcatcga acaacgcgtg gcgggcgagc aaaattttcc gtagtccacg
     6961 gttcatcgct ttacgcttag tggcaagatc ttcagggtta aaccagctga cgccaaagtg
     7021 attatgcaca ccgctggcgg aaataaatat ttttcgcgga ttgagagagt ctaatggcga
     7081 agagttactg gcatcgtaaa aagcatcact tctggcacga tacgtaccac cacaaagtat
     7141 tgctgttaca ttaggctttt cattcaacgc aacaaagacg cgatgtgagt aacagatgcc
     7201 ggtgaaggtg attgcatccg gaatcatgct tataacgagt ggtatctcct ggccattatc
     7261 aaagaagatc agatcatttt cattaaccat tccggcagcc agaattgcaa taggtaagtc
     7321 atcacgatga ttttttggaa cgtcatggat tactggcatg gatggcgcgg gtttattcac
     7381 cattacaata tagccaccca gtagggtcag tggcagaggt tcatcttcct gatggagatc
     7441 gcgacgaata gtcattacag aaacttccag cattcgcgct gcgtctttca aatggattct
     7501 gtcggttttc ttaagcagtt ccatcaaacg tcggatacgc tctttttgct tcgtttccat
     7561 caactctctc tccgtaagaa tgttataaaa ataacatttt ggcgttagta aacattgttt
     7621 tcattaccat actatacgac cgcccctttg aaatgaaaag ggtataggct gctgagtttt
     7681 gggcactaat cttgttaggt tgcttatgat tagatagtgt tggcctgagt gatgagactg
     7741 gaagtctatt aaataaacat cgggttgatg tgctaatcgc gatctcagtc acaaatgatg
     7801 caaaagtaac atgctaatgt tatttaaata ccattgttat gcgaaagttt ataagaggta
     7861 gtgaaatgga cattgcagtt attggttcca atatggtgga tctcattact tacactaatc
     7921 agatgcctaa agaaggagaa actctggaag cacctgcctt caaaatcgga tgtggtggga
     7981 aaggtgctaa tcaagcggtg gcggcggcta aattgaattc aaaagtcctg atgctgacta
     8041 aagtaggtga tgatattttt gcggataata ctattcgtaa tcttgaatca tggggaatta
     8101 ataccactta tgttgagaag gtcccctgta ccagcagtgg tgttgcaccg atctttgtta
     8161 atgccaactc gagcaatagt atattaataa ttaagggagc gaataaattt ctttccccgg
     8221 a
//
LOCUS       DACADA010000183         8195 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.185, whole genome shotgun sequence.
ACCESSION   DACADA010000183 DACADA010000000
VERSION     DACADA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.185"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            50..385
                     /locus_tag="ISB17_004152"
     CDS             50..385
                     /locus_tag="ISB17_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAP1982024.1"
                     /translation="MAILITQDRQVANLTPPEGKSRITVSVKSKVGSGLSVESRSGSQ
                     TKSWIYRPYLNGKQIKITLGSYPAMTLSQAREAHADALALVKQGIDPRYARKTEKQQN
                     EQMPIFSQL"
     gene            593..1369
                     /locus_tag="ISB17_004153"
     CDS             593..1369
                     /locus_tag="ISB17_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1982025.1"
                     /translation="MTLDHAALQGLIDHNPARLLKPAMFGASMGKPRERWLPQDELQM
                     LWKALDEAGFGGGSVATGGRGIASSVILSQAIANALRLIILTGVRRSEAVNMRWEQIN
                     GNRWTIPETKNGKSHIVTLHPLALSLLKTQRIISEGGWVFESLSKPSFPVTGDAITRA
                     LERLKTKYMAEVAPFSPHDLRRSIATGCAEYLDAPERLIELLLNHVPKDRLIRTYQVG
                     QQAEKLRNLFLKWGDFIEHEIIESDNGTPDNVIQISFGKR"
     gene            complement(1534..2454)
                     /locus_tag="ISB17_004154"
     CDS             complement(1534..2454)
                     /locus_tag="ISB17_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP1982026.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAMLKDKNPAKYVAAVNKLERVLE"
     gene            complement(2441..3244)
                     /locus_tag="ISB17_004155"
     CDS             complement(2441..3244)
                     /locus_tag="ISB17_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982027.1"
                     /translation="MSIQERHLPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYV
                     QNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGK
                     PQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPE
                     LAAYEIACGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNG
                     HPYFQYLDQSGIETGTGKRQIIPGGWLEPDYQITVPRTFKPEGVEDGSA"
     gene            3507..6605
                     /locus_tag="ISB17_004156"
     CDS             3507..6605
                     /locus_tag="ISB17_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1982028.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYHYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENGDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSEETEIAVLKDNNLEVIRTNPEFQTNKDHNRPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKYIYAHPSFVKPMLNYIVTDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTAADEVSEEMPVLQAAEPAAVYHLHAKETRKIKRAALILHDIGS
                     KQERKEWVEQFKAGKIDLLFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRT
                     YNHFRYGYVVDFADIRQEFDATNKAYFEELQAELGDEMEHYSRLFKSAEEIREEIEHI
                     KDVLFSFDTENAEIFTDQINQIQDRETALALKNALADARSLYNLIRLQGNTEFLQALD
                     FNKLNILYREASRRLDLLNLKADLEQGVDLSGLLNRALEEVIFEFHKVGEEELVLADE
                     LKETLRRTREALAANFDQQDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIH
                     GRIKELNRQNNLLRQKYRGDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKV
                     LNNSQILNNESYFERHMESCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGAR
                     TW"
     gene            6599..>8195
                     /locus_tag="ISB17_004157"
     CDS             6599..>8195
                     /locus_tag="ISB17_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAP1982029.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGGALKRFDYIVSNPPFKMDFSDFRDALDSKENQQR
                     FFAGIPKIKAKARDKMEIYQLFLQHIIFSLKPGGKAAVVVPTGFITAQSGIDKGIREH
                     LVQNKMLAGVVSMPSNIFATTGTNVSILFIDASNKEKVVLIDASNLGEKVKDGKNQKT
                     VLTECEEKRICEAFNNKWSEEDFSVVVSYDDIAAKNYSFSAGQYFDVKIEYTDMTPEQ
                     FAAKMKGFTENLNNLFEQSRELEV"
BASE COUNT         2285 a         2246 c         1969 g         1695 t
ORIGIN      
        1 atacaatgct aaacaagtac gccagcaagt acgcgagatg aggccaaaaa tggcaatatt
       61 gataacccag gacaggcaag tcgcaaacct gactccccct gaaggaaaaa gtcggataac
      121 cgtcagcgtc aaatcaaaag taggttccgg cctgtctgtc gaatcccgtt ccgggagtca
      181 aacgaagagc tggatttacc gcccttacct caacggaaaa cagattaaaa taacgttagg
      241 ctcctaccct gcaatgacat tgtctcaggc gcgtgaagca catgcagacg cgctggcgct
      301 ggttaaacaa ggtattgatc cgcgttatgc gcgaaaaacg gaaaaacagc aaaatgaaca
      361 aatgccgatt ttttcacaat tatgaaaaaa ctggctggca ttccgtgaaa ccaataaacc
      421 cattggggcg cgtactctgg ctgattatga gggaacatat cgccgccatc tggagaaagg
      481 tttaggttca gtacgcgtct gcgacctgtc cagagcctac atttatgaac atctcatacg
      541 gatcagaaag accagcgccg aaggtgtccg caagggactt attcttctca atatgacgct
      601 tgaccatgcc gctttgcagg gattgatcga tcacaaccct gctcgcttgc ttaaacccgc
      661 aatgtttggt gcctcaatgg gaaaaccgcg tgagcgttgg ttgccacaag acgagttgca
      721 aatgttatgg aaggcactgg atgaagcggg gttcggcggc ggatctgtcg caaccggagg
      781 gcgaggcatc gcgtccagcg ttattctttc acaagcgatt gctaacgcat tacgcctcat
      841 catcctgacg ggggtgcgac gttccgaagc ggtgaatatg cgttgggagc agattaatgg
      901 caaccgctgg acaatacctg aaaccaaaaa cggtaaaagt catatcgtga cgctacatcc
      961 tctggcgctt tctctattga agactcagcg cattatttca gaaggcggct gggtattcga
     1021 gtcgctcagt aagccaagtt ttcctgtcac cggtgacgca ataactcgtg ctcttgaacg
     1081 cctgaaaaca aaatatatgg cagaagtcgc tcctttctca ccacatgatt tacgccgcag
     1141 cattgctaca ggctgcgctg aatatctgga cgcaccagaa cggcttatag aacttctgtt
     1201 aaatcatgtg cccaaagaca ggttaatcag aacctaccag gtcgggcagc aagcggaaaa
     1261 attacggaat ctgtttctga aatgggggga ttttattgag catgagatca ttgagtctga
     1321 taatggtact ccagataatg ttattcagat cagctttgga aagagatagt ttcgtatttt
     1381 catgagtaga gatcgtctga taactcagcg atctcttact cgatggtgct atgtattgaa
     1441 ttaaatgaat atgtttgagt tattgccacg aagttattca tataaccaaa gcatcatggc
     1501 ccaattcaga gtccctgaac agcgctaaga atattactcc agcaccctct ctagcttatt
     1561 caccgccgcc acatacttcg ccggattttt atctttaagc atcgccagat tgcgttgctt
     1621 ccagcgaata gccggaagat gctgaatttc aggatagcga taccgcgacc agtcagggct
     1681 gttttgctta aaactgagca agaactcgcg atccagatcc gtaaaatgtg acttcaacgc
     1741 ctgcggcagc aaagtcgtta ctgacagaag cgattccagc gacgtttcct gctgagtcat
     1801 ccccgaaaat tcctgagcgt ataacgtggc aatccgatct gtatcccagt ttgaagccag
     1861 caactcggca atgggacggg gatggcctgc cagataacaa agaaaaccgt caaaaatttc
     1921 gcgcgtcagg cccggtctcc ccagcatatt caacacatca aacagatcgc gcggatgctg
     1981 acgatcaagc gcagcacata ttttaccgcc ataaagatcc gccagcgaga cgacactcat
     2041 ggtggtgaag ccatactcca tttccacacg ttcgcacaca ggcatttctg ccggaggcaa
     2101 caacagcccg cgccagaccg ggctaacctc gatcttaatc tttgcatcag cggtattcac
     2161 aatgatgcgc ttttcatccg ctttattttc ctgccgaatc gcggtgatcc ccggtctgcc
     2221 gttcagcgat gcagtaattc tcttcaaagc gtcgttgatc gccgccagat cggtatcgcg
     2281 atccgcaaag ctcttatacg ccagatcgat atccaccgat agacggggga aatcctgcac
     2341 aaataaattg attgctgtgc cgcctttcaa cgcgaaacag gattccttcg ccacatgcgg
     2401 cagagcggcc atcaacagtt taacctgcct ggcatagcta tcacgcagat ccatcttcga
     2461 ctccttccgg tttaaatgtg cggggaacag taatttgata atccggctcc agccagccac
     2521 caggtatgat ttgccgcttt cccgtacctg tttctatacc gctttgatca agatactgaa
     2581 aataagggtg tccattacgc cgggcaaggt aaagcatcac ccggttggtt ttaatcgaat
     2641 gactggcgcg aagcagtgcc tgcaatttgc gtgggctgag aagattcccc ccctgaaaca
     2701 gagcatcggc atgttcgaac gagatcaacg aaggaacgcc gcaggcgatc tcatacgcgg
     2761 ccagttccgg tgcactgcct gctaactgcc ggtcgccaat tttcagttcc gtagtaaatg
     2821 acgagacatc aacggtaagg cccgatgtat acagcgtgat aaatgttgct gattgttcga
     2881 aggcagcaaa ccatcccggc agtttgacgt aagccggaag cgcgagccag acctgcggct
     2941 tacccggttc ggtatagtgc gcatggccct gaagcgcaag gctggttagc cccgccaccc
     3001 gaacaggctt ttcccactgt gtttgaaggc agtaaaccgc gtctacccaa tccggctcac
     3061 ggcccgtccg gcagtacaca ccgtgagcga tgcgcctgag ccagccattc tgaacataga
     3121 gataagaaag cttacgatct acgccattgc gggtaagcca tgactggagc agcacctgac
     3181 cgggtagcgt gttttgcatc aaccagttta gatagtttgg caaatgtcgc tcctgaatag
     3241 acattagtag aaatatttaa accagtgtag gttcagggat acgccacagc aagaaatagt
     3301 ttaatgattc ctcatttcgt ccacttaaaa tcgacattga gcaaaaaata caaacttacc
     3361 tacattcagg caaagtgcga caaatagact cggtcggttt aaagagaacg tgtaacatag
     3421 atgcaaatcg aacagacatt gttgccagcc gttccaaggt tgtggggcgc tgcatggcct
     3481 gagacgtgat aaatacaggg aacacaatga aattcactga agatacccga gtaaaaatcc
     3541 ccgtcatcct ccacctgata cggctgggct accactacct gtcccttaaa gaacaacgct
     3601 gggacaaaga aaccaatatc ttccctgacc tcttcaccgc tgcgattggc agaataaatc
     3661 ccggacttgc gccagacgac atcggacgcc tgttaaaaga cctcacgctg cttctcgaca
     3721 acgatgatct gggccgcgcc ttcttcgaaa aactcagtga ccgttccggt atcaaactga
     3781 tcgactttga taacttcaac aacaacagtt tccatgtcgt caccgaactg acatgcgaaa
     3841 acggtgatga agaattccgc ccggacatca cgctgctgat caacggcatg ccgctaatct
     3901 ttatcgaagt gaaaaaaccc aacaatcggg aaggcatcgt cgcagaacgc aaccgcatca
     3961 acgaacgctt taaaaacccc aaattcaaac gcttcgccaa catcacccag ttcatgatct
     4021 tctccaataa catggagtat gacgacagcg acccggagcc gattcaaggg gcattctacg
     4081 ccagcagctc ttacagcaac ccggtattca actacttccg cgaagaagaa ctcttcaacc
     4141 tgaccaactt actgaaaccg ctgtcagaag aaaccgaaat cgccgtcctg aaagacaaca
     4201 acctggaagt gatccgcacc aacccggaat ttcagaccaa caaagatcac aaccgcccga
     4261 caaaccgcct ctgcaccgcg ctgttgagcc gggatcggct ggcatttatc ctgcgctatg
     4321 cgctggtgta tgtagccgaa ccgggcggca tccaaaaaca catcatgcgc tacccgcagc
     4381 tcttcgccac caaagcgatt gagcgcaagc tgaacgcggg tgtgaaaaaa ggcattatct
     4441 ggcacaccca gggcagtggg aaaaccgcat tggcatacta caacgtgcgc ttcctgacag
     4501 attacttcca gaatcagcag gtgatcccga aattctactt tattgtcgat cgtcttgatc
     4561 tgctccagca ggcgcagcgt gaattcaccg cccgtggctt aatcgttcac cttatcgact
     4621 cccgcgaagc cttcactcgc gatatcaaag caacgcaggc gctgcacaac caccacggca
     4681 aagcggaaat caccgtcgtc aacatccaga aattccgcga tgacccggat gtcgtcacca
     4741 ccaaagacta cgacatcaac atccaacgcg tgtacttcct cgatgaagtc caccgcagct
     4801 acaaccccaa agggagcttc ctggcgaatt taagccagtc ggacaccaac gccatcaaaa
     4861 ttggcctgac cggtacgcct ctgctgggca cagactacaa ctcccgcgac ctcttcggcg
     4921 actacatcca caaatactac tacaacgcct ccatcgccga cggctacacg ctgcgcctga
     4981 tccgcgaaga gatcgccacc aactaccgaa tgatcctgca acaggcgcta aaagaggtgg
     5041 aagtcagaat gggcgatgtg gatcgcaaat acatctacgc ccatcccagc tttgttaaac
     5101 cgatgctgaa ctacatcgtc accgactttg ccaaaagccg gggcgcgctg aatgatgaca
     5161 ccatcggcgg catggtgatt tgcgacagct ctgaacaggc caagcagatg tttgagatat
     5221 tcaacactga ctacgccgaa tcgcccaccg ccgccgatga agtctccgaa gaaatgccgg
     5281 tattacaggc cgcagaacct gccgccgttt accacctcca cgccaaagaa acgcggaaaa
     5341 tcaaacgcgc cgccctgatc ttgcacgaca tcggcagcaa gcaggagcgc aaagaatggg
     5401 tggagcaatt caaagcgggc aagattgacc tgctgttcgt ttacaacatg ttgctcaccg
     5461 gcttcgacgc caaacggctg aaaaaactct acctcgggcg ggtgatcaga aagcacaacc
     5521 tgctccaggc gctgacccgc gtcaaccgta cgtacaacca cttccgctac ggctacgtgg
     5581 tggacttcgc cgacatccgc caggaatttg acgctaccaa caaagcctac ttcgaagagc
     5641 tacaggctga acttggcgac gaaatggagc actactcccg cctgttcaaa tcggcggaag
     5701 agatccgcga agaaatcgaa cacatcaaag acgttctgtt tagcttcgat accgaaaacg
     5761 cggaaatctt cactgaccag ataaaccaga tccaggaccg ggaaaccgcg ctggcgctaa
     5821 aaaacgcgct ggccgacgcc cgcagcctgt acaacctcat ccgcctccag ggcaacactg
     5881 agtttctcca ggcgctggat ttcaacaaac tcaacatcct ttatcgcgaa gccagccgcc
     5941 gcctcgactt actcaaccta aaagcggatc tggaacaggg cgttgacctc tccggcctgc
     6001 tgaaccgggc gctggaagag gtgatctttg aattccataa agtgggtgaa gaagagctgg
     6061 tactggccga cgagctgaaa gagacgctgc gccgcacccg cgaagcgctg gccgccaact
     6121 tcgaccagca ggacccgcag ttcgtcagcc tgaaagacga actggagcgt ctgtttaaga
     6181 aaaagaaact cagcgaagtc acccagcaag aaatggttgc gaatatcggc acactcaata
     6241 aaattcatgg ccgcatcaaa gagctaaacc gccaaaacaa cctgctgcgc cagaaatacc
     6301 ggggcgacgt gaaatacgcg cgtacccaca aacgcctgcg cgagcgcggc ggcctgagcg
     6361 agccggagcg taaaatcttc gaagcactgc ttagcgttaa gctggatgca gatgaaaaag
     6421 tgctgaacaa cagccagata ctgaacaatg aaagctattt tgagcggcat atggagtcat
     6481 gcgtcatcac gcactttgag gatgagcacc atatcactct gacctccagt gcggtaagcg
     6541 acatcaaccg cctgattgtg gcggaatacc tgaacgaatt taataccgga gcacgaacgt
     6601 ggtagaactg gaatttagag acaaaaccaa agccctgatc gacagcctga aaagcatctg
     6661 tgcgaactac ggcctgggca acgacggtaa cgaattcaaa attatcactc aggcattcct
     6721 gtacaaattc ctgaacgaca aattcgcttt tgaggccaaa cagaaagata aaagtatcgc
     6781 cagcgccgaa agctgggaag atgcgctcag cgcaatgagc gaagatcaac tgaaaaagct
     6841 gcaacagcgg atggccccgg ataccgcccg cctgaagccg caccatttta ttcgttactt
     6901 gtataaccga caaaacgcag cggactttgc cagaactttt gacgacaccc tgatggacat
     6961 cgccgccacc aacaacgacg tcttcgcggt caaaaccgac ggcggcgcga aagtggtgct
     7021 gtttgagcgc ctcagccagt acatcgccga cgaatcaaag cgcgatgatt tctgccgcgc
     7081 catcatcaac aagctggccg atttcagctt cgaacgcatc ttcacgcaga aattcgactt
     7141 ctacgccacc atctttgaat acctgatcaa agactacaac agcaactccg gcggcaaata
     7201 cgcggaatac tacacgcccc acgccgtggc gcgcatcatg gcggaaatcc tggtgccgaa
     7261 agcgcagcag ggcgtggtgc gcaacgtaag ctgttacgac ccgtccgccg gttccggcac
     7321 gctgctgatg aacgtggcgc acgccattgg ggaagatcgt tgcagcatct ttgcccagga
     7381 tatctcgcag aaatcctcca gcctgctgcg tctgaacctg atcctcaaca acctggtgca
     7441 ttcgatccct aacgttatcc agggcaacac tattctgcat ccgttccaca aggacggcgg
     7501 cgcgctgaaa cgctttgact acatcgtctc caacccgccg tttaagatgg atttcagcga
     7561 ctttcgtgac gcgctggaca gcaaggaaaa ccagcaacgt ttctttgccg ggataccgaa
     7621 aatcaaagcc aaagccaggg acaaaatgga gatttaccag ttgttcctgc aacacatcat
     7681 tttctcgctg aaaccgggtg gcaaggcggc ggtcgtcgtg ccgaccgggt ttatcaccgc
     7741 gcaatcgggc attgataaag gcattcgcga acatctggtg caaaacaaga tgctggcggg
     7801 cgtggtttcc atgccgtcga atatcttcgc caccaccggt accaacgtgt cgatcctgtt
     7861 tattgacgcc agcaacaaag agaaagtggt gctgatcgac gcctcgaatt taggtgaaaa
     7921 ggtcaaagac ggcaaaaacc agaaaacggt gctgacggag tgcgaagaaa aacggatctg
     7981 cgaagcgttt aacaacaagt ggagcgaaga ggatttctcg gtggtggtca gctatgacga
     8041 catcgccgcg aaaaattact cgttcagcgc cggacagtat tttgatgtga aaatcgaata
     8101 caccgacatg acgccggaac agtttgccgc gaagatgaaa ggatttacgg agaatctgaa
     8161 taatctcttt gaacagtcgc gcgagttaga agtgc
//
LOCUS       DACADA010000184         8171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.186, whole genome shotgun sequence.
ACCESSION   DACADA010000184 DACADA010000000
VERSION     DACADA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.186"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(241..432)
                     /locus_tag="ISB17_004158"
     CDS             complement(241..432)
                     /locus_tag="ISB17_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982030.1"
                     /translation="MSEDKKVVNLLSELSAALDNINPPRVKTTGDKDALNTEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            570..920
                     /locus_tag="ISB17_004159"
     CDS             570..920
                     /locus_tag="ISB17_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HAP1982031.1"
                     /translation="MKLQRNSSQQRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVD
                     RLLHGQSFIKQADL"
     gene            931..1209
                     /locus_tag="ISB17_004160"
     CDS             931..1209
                     /locus_tag="ISB17_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982032.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARKTNGDEPYIEIICMDSEAEIQKAD"
     gene            1221..1463
                     /locus_tag="ISB17_004161"
     CDS             1221..1463
                     /locus_tag="ISB17_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 domain-containing protein"
                     /protein_id="HAP1982033.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            1460..1573
                     /locus_tag="ISB17_004162"
     CDS             1460..1573
                     /locus_tag="ISB17_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982034.1"
                     /translation="MSIFMSWIVLIISVVCAIGIVRIIHSVKKIERFFTGE"
     gene            1667..2077
                     /locus_tag="ISB17_004163"
     CDS             1667..2077
                     /locus_tag="ISB17_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982035.1"
                     /translation="MHAKEEGIIRALKEISKTENEVAKKAIANNHMDVATHTLIVARV
                     TAEAAEIIAKQDAELAVLRTQPVTGLDLSNTGRLIYTIGSELQRYTIIAGLQDKYLIT
                     PHPIRESEILTNLRLIERSQVAFIDDAQCTVFNA"
     gene            2101..2304
                     /locus_tag="ISB17_004164"
     CDS             2101..2304
                     /locus_tag="ISB17_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982036.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            2301..2567
                     /locus_tag="ISB17_004165"
     CDS             2301..2567
                     /locus_tag="ISB17_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP1982037.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            2564..2863
                     /locus_tag="ISB17_004166"
     CDS             2564..2863
                     /locus_tag="ISB17_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982038.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAALALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEVPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            2875..3492
                     /locus_tag="ISB17_004167"
     CDS             2875..3492
                     /locus_tag="ISB17_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1982039.1"
                     /translation="MHTQKNRLPCRNQSGYISAAPHKTGAGFRSLLTTQAHNRASGFF
                     VRTVLPRFFRVRIMAGRTGPTSVGPGSCVAGTANLVRLATHSFAALDGEFSQLTTQGA
                     TPWQTANSTALSRSVVTSRLKSTADFPAHHASRKSCTSICCMSAATHYQTFIPPLFSA
                     IWRMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSVTRGLPQ"
     gene            3489..3854
                     /locus_tag="ISB17_004168"
     CDS             3489..3854
                     /locus_tag="ISB17_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982040.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            3861..6683
                     /locus_tag="ISB17_004169"
     CDS             3861..6683
                     /locus_tag="ISB17_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAP1982041.1"
                     /translation="MTAEYLRDWQQPRHAVGREGTGIPAPESALSSWLDAYRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDSTDGRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQGQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            6760..7719
                     /locus_tag="ISB17_004170"
     CDS             6760..7719
                     /locus_tag="ISB17_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP1982042.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVAPAIREVCGVNATFIADGVPQFALVNGLYAMDKE"
     gene            7724..8038
                     /locus_tag="ISB17_004171"
     CDS             7724..8038
                     /locus_tag="ISB17_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HAP1982043.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQDNQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2231 a         2195 c         2051 g         1694 t
ORIGIN      
        1 aacaaagatt ctgttgtcaa tggaagagat atagatactc ttcctgcaca aatcaaaccg
       61 atagcctatc aattgcgtaa tcgttttaaa aatgaaggtg ggattatatt tgactttccc
      121 gcagctgtag ccgtgagctt ctctcggggg gatatacttc actcatttcg ttttgctcta
      181 tcaggtgacg agacattatg gcagctagaa gatgccggga gcagcccggc ataaatcaaa
      241 ttaaaaatca accagttgtg tgacaggtgg gtatttacgc tgaattttgt cagttactaa
      301 cgccagtaat atatgaatct ctgtgtttaa tgcgtcttta tcacccgtcg tttttaccct
      361 gggagggtta atgttatcca gagcagcact aagttcactc aacaaattaa caactttttt
      421 atcttctgac atgaaaacct cttaacaatt taattaaaca taagccagga cctgaaccgg
      481 ccagtccaag tcctagacac acaaaaaaaa taccacaaat caatacaagg tgacacgagt
      541 acaccttacc gaaggtacag agttttaata tgaaactaca gcgcaattct tcacagcagc
      601 gcttccgcaa cggtgcggaa cgccatgcta accgtttcgc taccagtgca tcacgtagca
      661 actctcgcta cagcctgagc gaaacacacg caacgccgga tggctaccca gtaaaacaaa
      721 ttggtgaaca cacttggctg attgaaaaag caggaatcgt ggttcacaga tgccagcgca
      781 acccatttac cggaaaccgc atttttgcac tgagcaacgg cgacaatcag ttcgggcagg
      841 atttcacatt atacgaagca cttcgcacgg ttgatcgtct gcttcacggg caaagtttta
      901 ttaaacaggc tgatttataa caggtgcttt atgaccaaag agcatgcaca aggtgtattt
      961 atccgtttta ttgattttcg cggtgaactg ttattacgcg catcagccat tgacggagtt
     1021 gttccatccg aaaaaaatgc agctacttac gtttatctga acggcacgcg cctgaccgta
     1081 gaacttccgt accagactgt acacgaaatc attagcgaag cggaaaaagc acgtaagact
     1141 aatggcgatg aaccctatat cgaaattatt tgtatggatt cagaagcaga aattcagaaa
     1201 gcagattaaa gggcgttgcg atgggcaaag aatataaaac tctcattaac aaagcacttg
     1261 agcgttttta ttttcgctta agtgcatcag gcgctcatgc tgaacgtgca gcccgtgact
     1321 cattgaccag ggcaatccgg agtctgtatg acgtggcttt ttacgctgat gatctggatg
     1381 cacttaacga actttccgag ctgatctgtg ccgcagaatg cggggaacat attgaaccgt
     1441 ataagctggg gaatatcgca tgagtatatt tatgtcatgg attgttctga ttatttcagt
     1501 ggtctgcgca attggcattg tgcgaattat tcattcagta aaaaagattg aacgtttttt
     1561 cactggtgaa taacgataca aataaaacat caaattaaat aagaaaacgt gaaaaccatc
     1621 cgtattaacg gaggtattcg cacacgcaaa taacggagat acaaaaatgc acgcaaaaga
     1681 agaaggtatc atcagagcac tgaaagaaat ttcaaagaca gaaaacgaag tagcgaaaaa
     1741 agccatagca aataatcata tggacgtcgc aacccacaca ctgatagtcg caagagtcac
     1801 ggcagaagca gccgaaatta tcgcaaaaca ggatgctgaa ctggcggttc tcagaacaca
     1861 accagtcacc ggactggatt tgtctaacac cggacgcctt atttacacaa ttggctcgga
     1921 gctacagcga tacaccatta tcgccgggtt acaggataaa tacctgatca ctcctcaccc
     1981 cataagggaa tcagaaattc tgacaaatct ccgcctgata gagcgctctc aagtcgcatt
     2041 cattgatgac gcacagtgca ccgtatttaa cgcatagagt tactggacaa agggggcgca
     2101 atggcaatta agcattttcc cgtcgttcgc tttacctcca gagggcgcga atacgaggtc
     2161 gacgaacgcc tgattaccac tatcgacaaa catcgttcgg aaaaggatgc acaccacatc
     2221 tacctcactg acggcactta cttctgcgcc actaatgtgg cgcgggtaaa ccttatccga
     2281 caggtacagg agccacgcag atgaccattc tggactacat cgctactcat ccggggtgta
     2341 gcggcggaga gatcgccgca gcactgaata ctccaaccac agccattaat gctgagttac
     2401 gccaactttg gcgcggcggc ttagtcatca gaacaaaccg cagcacaggt ggtcgcgctc
     2461 gcaaaactgg aggccaggct tcttaccacg taaacccgat gccgttcggg tgtagcaatc
     2521 cacttactca catgtttaac cagctactga aggaagccag aacatgagca ccatcaacca
     2581 ccagaagcta cgcgaactgg catttgccct gcaacgaatg gcaacacctc aaaaattact
     2641 ggcatttcgc gcaatgctct cgccgtctgc tgcgctggca ttgctggatg agctggagca
     2701 cgccagaacc acggctcctg ccattcgcct gacgctccat catgaaatcg ctgatttctg
     2761 cgcgacattg gaggtgccag gcgaaccgga aacgccggaa gcaatacagc aagagctgct
     2821 gcaacgtatt gacaaggttt ttgatttttt tctgaaccag taagaaacca gagcatgcac
     2881 acacaaaaaa accgcttgcc atgccgcaat cagtcaggtt acatttccgc tgcacctcat
     2941 aaaacgggtg ccgggtttcg cagcctgctg actacacaag cgcacaaccg cgccagcggt
     3001 ttttttgtgc gtactgtatt gccacgtttt tttcgcgtca gaattatggc ggggcgtacg
     3061 gggccgactt cggtcgggcc gggttcttgt gtagccggta ctgcgaacct cgtacgtctc
     3121 gccacccaca gtttcgcagc tctggatggt gagttttcac aacttactac acaaggggcc
     3181 acaccatggc aaaccgcaaa cagcaccgcg ctatcgcgga gcgtcgtcac atccagactg
     3241 aaatcaaccg cagactttcc cgcgcatcac gcgtcgcgca aatcatgcac atcaatatgc
     3301 tgcatgagcg cagccacgca ctatcaaaca tttattccgc ctctgttttc agctatctgg
     3361 cggatgatct gcacgagctt caacagctca tccagcagca aaacaaactc cattaattcc
     3421 tgttccgggc ctttcctgca ccttgcggcg ggaggccttc gcacatctgt aacaagagga
     3481 ttgccgcaat gattctcgcc aacgactttc ttgaatacct gctcaataca gaacgtgatc
     3541 ttgccgctcg cgtgcgtgat cgttatgaca tgtacctgaa atccctgcct gtaccgcagc
     3601 tcgctgacgg aaagattgtt attgatggtc gctacatgat tgacagccac gagggaaatt
     3661 acaggcttta ccgcattgaa ggtggcaccc cgtccgttat tggcatttac cagcgcccat
     3721 cctctgcaat cgtcgatgtg attgccgaca gcatccgcat cacacatcgc catgccgaca
     3781 cagaagacac cgtgctggaa attcagcggc tggctacagt ctgccgcgac accctgaatg
     3841 gcatgacgaa gtaaatcact atgacggcag agtacctcag ggactggcaa caaccgcgcc
     3901 acgcagtggg gcgtgaagga acggggatcc ccgctcctga atccgcgctt tcctcctggc
     3961 tggatgccta ccgggcagag aacgagcgcc gccaggaaat ggctgatgcg gcgttctcag
     4021 ccacgccgct gggcaacctg attaataaaa gcctggacgc acaggaaaaa caggacaaaa
     4081 ccatcacact ggcaggagac gccagaaaac aggcacgtgg cgcggtggat gaagccatgg
     4141 cctcgctgcg cctgctgccg tcctatctgc gcgatccgct tattcgccac ctctccttcc
     4201 tgcgcaaaaa acaggaagcc gatcgccgga aaggcaaaaa gagctggcag gcggaacgct
     4261 atgcacgcgg aaccctgcgc aaaatattcg aacgtctgga tagcactgac ggacgctggc
     4321 tgacaccggg ttatcgctcc cttgccggac gcgaacgcct ggacgatttg ctttacctgc
     4381 cgcagctcaa caaacaccag atacagacgc tggccaccat gacggcggcg atgttcagca
     4441 gcaccttcga aaaactctgc gatggctttg gcgcgaccga tggcgagctg accatggatg
     4501 taaccctgaa ggcgtatcag atgctggccc gcatggcgtt acacctgcac gccatgcctc
     4561 cacattatga cgcactgaca acagacaaag accggaggaa cgaaccggac acggagctgc
     4621 tgccgggcgc aatccttcgc ctgacctgtg cggaatggtg gaaacgcaaa ctgtggctgt
     4681 tacgttgcga gtggagagaa gaacaactcc gcgccgcctg tctggtttcc agaaaaacat
     4741 caccctatct gagccaggac gcgttaagcg agtttcgcgc acagcgcgag aaaacacgcg
     4801 atttcctgaa aagtttcatg ctggaaaatg aagacgggtt cacgattgat ctcgagacag
     4861 tgtattacgc gggagtaagt aacccggttc accgtaaggc agaaatgatg gccaccatga
     4921 agggactgga acttctggcc gaagcccgtg gcgacaaagc ggtgtttctg actgtcacct
     4981 gcccgtcaaa ataccacgcc acaacggaga acggtcatcc gaatcccaaa tggaacgggg
     5041 ccacaatgcg cgactccagc gattacctgg ttaacacgtt ttttgcggcg gtccgcaaaa
     5101 aactgaaccg cgacggcctg cgctggtatg gcatccgcac ggtggagcct catcatgacg
     5161 gcaccgtgca ctggcatatg atggtctttg cacatccgga agaaatcgac accattgtgt
     5221 cccatacccg cgatattgcc attcaggaag accgccacga gctgggcaat gatattactc
     5281 cgcgctttaa ggtggagtat gtcgacggct caaaaggcac gccaaccagc tacatcgcca
     5341 cctacatcgg aaagaacctg gacagccgcg ccgtggatgg catcgacccg aaaacgggca
     5401 agccacgcgt ggaccacgaa accggaaaat caatggccga gagcgtggag cgcgccatcg
     5461 gctgggcgcg ccttcaccgg gtccgtcagt tccagttctt tggcatcccc tcccgtcagg
     5521 tgtggcgtga actgcgccgc cttgccagcc agatggcacg caatccggaa ggcccgcaac
     5581 ggctgaaaga tgatgcaatg gatgcggtac tcgctgccgc cgatgccggg tgttttgcca
     5641 cctacattga aaaacagggt ggcgtgcttg ttccacgcaa ggactacctg attcgcaccg
     5701 cctacgacct cgcagatgag ctgaacgatt acggcgaaca gagcgtacag atttacggga
     5761 tctggtcacc actcatcggg gaatcctccc gtgtgtgcac gcatccggat aactggaagc
     5821 tggtaagacg taaaccggaa gcggaagaca gcgcccgcga aaatggtttt gaccttcagg
     5881 gcggccctgc cgccccttgg actcgtggca ataactgtcc ccgtgtacag gaaacggaca
     5941 acaacgggac agaacagccg gaagaacggc cagcaccgtg gccgcagctc cctgacggcg
     6001 ttgaagtgaa cgaatggatg cgctcactga aacgacacga acgccgggcg ctgatgcgtt
     6061 cgcttcgtga caaacaggca aaaaacagca gcgatgaaat gcagagctgg acacagagcc
     6121 gcaaacagca gcggcctttg cctgataacc acgaattact cgctaaagaa tggcgggagt
     6181 ctgctgaatc tctcggcctg catatcggtg aacaacagat gcagcacctg ttacggggcg
     6241 gcagtctgta cgttgacggc agcatcattg caccgcaggg atttgaaatt gtacgcaaac
     6301 cggatacccg cccggacagc cgaatcacgc agctctggca gcgcctgagc cgtaatcatg
     6361 gcgtaagcag cacggagatc cgccataacc cggtcgccag ctatctggca cagctggggg
     6421 catcagaccc tgaagccgcc gcacgcctgg catccacact tcagcagggc cagaacacca
     6481 tgaaaacacc cgttaccgtg ctttctgaca tgctgcgcgc catccgcgac gcagagcacg
     6541 cacagagaat cagtgaaacc actgaacgcg ccagccgcaa agcagacctg ctgcggggtg
     6601 gcctgaccag tggaaacaaa aaacagacag aaacgggact tacgaatccc gtaaatgagc
     6661 aaaaaacgcg cagcgatata tgaagcgcgc acaaaacagg caaaaacggg atttcagaat
     6721 cccgtaaacg attaattaat caacataagg aaaagcgaca tgaaaatttg tatcgacgac
     6781 ggctccacca acatcaagct ggcatggact gagaatggcg aacgccgcaa cgccatcagc
     6841 ccgaacagct tcaagtcgga atggtctgcg ccgttcggtg gcatgcagcc cgcgaactac
     6901 atgcttgatg gcgtgcgcta tggttttgat ccggttagcg atcgctttgt ccagacgacc
     6961 gacacgcaat accagtacag cgatgtgaat gtaatcgcca ttcatcacgc gctggtcaaa
     7021 tcaggcatca cgccacagga ggtggatgtg gttgtcaccc tgccactgag cgagtatttc
     7081 gacacaaacg cacagccgga catggccaac atcaaccgca aaaaagcgaa cgttatgcgc
     7141 ccggtggaat accagaacgg cgaagcattc actatccgta acgtgcgggt tatgcctgaa
     7201 tccattccgg ctggctttaa agcactggct gacatgagtc cgtttgaatc cctgctgatt
     7261 gtggatttag gcggaaccac gctggatgtg gcaaaggttc aagggcaact ggcaggtatc
     7321 agccaggtgt tttgcgatcc acacgtaggc gtttccctga tggccgatgc cgtactgtcg
     7381 gtgatggcca ctaacggtat gcgcaccagt caccacatcg ccaataccat tatcgaacat
     7441 cgccatgatg aagcctggct gcgccagcac atccacaatg acgcgcatta cgccagcctg
     7501 atggcggtta ttcgtgaaaa ggaagaaaca ctgaaacaac gcgtgatccg cgcgctggcg
     7561 gttttttcgg gttacgggcg ggtgatggtt gtcggtggag gggcggagat tgtggcaccc
     7621 gctatccgcg aagtctgcgg agttaatgcg actttcatcg cggacggggt gccacagttt
     7681 gctctggtta atgggctgta cgcaatggac aaggagtaaa ccaatgacga caccaaccag
     7741 acggataagt ttctatctga agcccgccgc cgtcaagagt gaacaggagg cgtgcaatta
     7801 cctcgatagc ctgccagcct ccgaacgcag ccgcgcgcaa cgcgcggcct ttctggctgg
     7861 gctagcgctc ataaagcgca acccagcatt tgcctactgg atggccgaat ggccagagga
     7921 caacctcccc ttcaataccg ttagcatcaa ggaaacatac caggataatc agcctagcgg
     7981 agttgaatgc aatttttata aaataaaaaa gaatattcag accttattcc cagaataatc
     8041 tgataattgt atgtgaacta ataaaataca gaccaacaca tcattctggc acccttgcta
     8101 acagagtgcc aatgtttcaa atcatgatga aattgccgaa gtttttttat cgtgctgaag
     8161 taccgcaaat g
//
LOCUS       DACADA010000185         8110 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.187, whole genome shotgun sequence.
ACCESSION   DACADA010000185 DACADA010000000
VERSION     DACADA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.187"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            230..1213
                     /locus_tag="ISB17_004172"
     CDS             230..1213
                     /locus_tag="ISB17_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HAP1982044.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1285..2433
                     /locus_tag="ISB17_004173"
     CDS             1285..2433
                     /locus_tag="ISB17_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HAP1982045.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2457..4133
                     /locus_tag="ISB17_004174"
     CDS             2457..4133
                     /locus_tag="ISB17_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HAP1982046.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQIDPKGNIFLPNVGPVKVAGVSNSQLNTLVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4143..4883
                     /gene="kdsB"
                     /locus_tag="ISB17_004175"
     CDS             4143..4883
                     /gene="kdsB"
                     /locus_tag="ISB17_004175"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP1982047.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4880..6907
                     /locus_tag="ISB17_004176"
     CDS             4880..6907
                     /locus_tag="ISB17_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HAP1982048.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLQRNHQQQRNGYLWSP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWLAEAQQKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIVYKPHPDVLVGNCKGDIPAELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTLADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6942..>8110
                     /locus_tag="ISB17_004177"
     CDS             6942..>8110
                     /locus_tag="ISB17_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP1982049.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYYG"
BASE COUNT         2079 a         1951 c         2104 g         1976 t
ORIGIN      
        1 tgggcaaaat ttggttcccc ttctcgcctg taatattgca gcatcttcgc acctccatga
       61 gacattgcga cttaataaga aggtgataag tcctgcatat aagcatggac tgaccatggt
      121 tttatattca ttaaaaattt tgttacaacc cattgattta gcataaataa attatagtgg
      181 gttcgggtta gttgtgactg tggcattatt tccgtgcaaa ggagctgata tgtctgaaag
      241 acatttacct gatgaccaga gcagtactat cgatccatat ctaattacct ctgttcgcca
      301 gactctggca gaacaaggcg cagcattaca aaacttgtct aaacaactgg attccgggca
      361 gtaccagcgt gtccttaatt tgataatgaa ctgtaaaggg cacgttattc tttcgggaat
      421 gggtaaatca gggcatgtcg gtcgtaaaat gtcagcgacg ctggcctcta cgggtacgcc
      481 tagtttcttt attcatcctg cagaagcttt ccatggcgat ctgggcatga ttacgcctta
      541 cgatcttctg atccttattt ctgccagcgg tgaaacggat gaaatcctca agctagttcc
      601 ttcactgaaa aatttcggca accgaattat cgccattacc aataatggaa attccacgct
      661 ggcgaaaaat gctgatgccg tgctggaact ccacatggcg aatgaaacct gcccgaataa
      721 tcttgcacca acaacgtcta ccacgctgac gatggcgatc ggcgatgcgc tggcgattgc
      781 catgatccac caacgcaaat ttatgccgaa tgattttgcg cgctatcacc cgggcggttc
      841 attaggtcgt cgcctgctga cccgcgttgc tgatgtcatg cagcatgatg ttcctgcggt
      901 acagctggat gcgtcattta aaaccgtgat tcaacgtatc accagcggat gccagggaat
      961 ggtgatggta gaagacgcag aaggtgggct agcgggcatt atcaccgacg gtgacctgcg
     1021 tcgctttatg gaaaaagagg attctctgac atccgccacg gctgcgcaga tgatgacacg
     1081 tgaaccgctg acgctaccgg aagacaccat gatcattgaa gcggaagaaa aaatgcaaaa
     1141 gcaccgcgtc tcaacgttat tggtgaccaa caaggcaaat aaagtcactg gccttgtgcg
     1201 cattttcgac taattaagca acggggtgcg agaggttagt ctctcattga gtttgcgccc
     1261 cactgaaact ttgataatcg ttacatgttg ataaaagtga agtctgccgt atcctggatg
     1321 cgtgctcgtc tgtctgccat ctcactggca gatatccaaa aacacctggc gaaaatcatc
     1381 attctggcac cgatggcggt gctgctgatc tatctggcta tcttcagcca gcctcgctat
     1441 atgagcgagt cgaaagtcgc cattaaacgc tcggatgatt taaacagcgg cagcctgaat
     1501 tttggtctgc ttctgggtgc ctctaacccc agttccgcag aagatgcgtt gtatctgaaa
     1561 gagtacatca actcgccgga tatgctggcg gcgctggata agcaactaaa ttttcgtgaa
     1621 gcgtttagcc acagcgggct cgattttctt aatcatctta gcaaggatga aaccgcagaa
     1681 ggcttcctga agtactacaa ggaccgtatc aacgtctcgt atgacgataa aaccggatta
     1741 ctgaatattc agacgcaggg ctttagcccg gaatttgcgc ttaagtttaa ccaaaccgtg
     1801 ctgaaagagt cagagcgctt tatcaatgag atgtcacatc gcatcgcgcg tgaccagctt
     1861 gcctttgcag aaacggagat ggaaaaggca cgccagcgtc tggacgccag caaagcggaa
     1921 ttgctctctt atcaggacaa caacaacgtt ctggatccac aggcacaggc acaggcggcg
     1981 agcacgttag tgaatacgct gatgggccag aagatccaga tggaagcgga cctgcggaac
     2041 ttgctgacgt atctgcgtga ggacgccccg caagttgtga gtgcgcgtaa tgcgattcag
     2101 tcattgcagg cacaaattga cgaagaaaaa agcaaaatca cagcgccgca gggtgacaag
     2161 ctaaaccgta tggcggtgga ttttgaagaa atcaaatcaa aagtagagtt caacaccgag
     2221 ctgtacaaac tgaccctgac ctccattgaa aagacccgtg tagaagcggc tcgtaagctc
     2281 aaggtgctgt cagtgatcag ttcgccacag ttgccgcagg aatcgtcttt tccaaatatc
     2341 ccttatttga tcgcctgctg gttactggtg tgctgcctgc tgttcggcac cctgaaactg
     2401 ttgctggctg ttattgaaga tcaccgagac taacgctgtc gctgaatgag tttgtgatga
     2461 aattatttaa atcaatttta ctgattgccg cctgtcacgc ggcgcaggcc agcgcgacca
     2521 ttgatattaa cgctgaccca aacctgacag gagccgcgcc gcttaccggt attctgaacg
     2581 gacagaagtc ggatacgcaa aacatgagcg gcttcgacaa tactccaccc cccgcaccgc
     2641 cggtggtaat gagccgtatg tttggtgctc aacttttcaa cggcaccagc gcggatagcg
     2701 gtgcgacggt aggattcaac cctgactata ttctgaatcc gggtgatagc attcaggttc
     2761 gcttgtgggg tgcgttcacc tttgatggtg cattacagat tgatcccaaa ggtaatattt
     2821 ttctgccaaa cgttggtccg gtgaaagttg ccggggttag caatagtcag ttgaacactc
     2881 tggttacatc aaaagtgaag gaagtgtacc agtccaacgt caacgtctac gcctccttat
     2941 tacaggcgca gccagtaaaa gtgtacgtga ccggatttgt gcgtaatcct ggtctgtatg
     3001 gcggtgtgac gtctgattcg ttactcaatt atctgatcaa ggctggcggc gttgatccag
     3061 agcgcggaag ttacgttgat attgtggtca agcgcggtaa ccgcgtgcgc tccaacgtca
     3121 acctgtacga cttcctgctg aacggcaaac tggggctttc gcagttcgcc gatggtgaca
     3181 ccatcatcgt cgggccgcgt cagcatactt tcagcgttca gggcgatgtc tttaacagct
     3241 acgactttga gttccgcgaa agcagcattc ccgtaacgga agcgttgagc tgggcgcgcc
     3301 ctaagcctgg cgcgactcac attacgatta tgcgtaaaca ggggctgcaa aaacgcagcg
     3361 aatactatcc gatcagttct gcaccaggcc gtatgttgca aaatggcgat accttaatcg
     3421 tgagcactga ccgctatgcc ggcaccattc aggtgcgggt tgaaggcgcg cactccggtg
     3481 aacatgccat ggtactgcct tatggttcca ctatgcgtgc ggttctggaa aaagtccgcc
     3541 cgaacagcat gtcgcagatg aacgcggttc agctttatcg cccatcagta gctcagcgtc
     3601 agaaagagat gctgaatctc tcgctgcaaa aactggagga agcctcactt tctgcccagt
     3661 catccaccaa agaagaagcc agcctgcgaa tgcaggaagc acaactgatc agccgctttg
     3721 tggcgaaagc acgcaccgtg gttccgaaag gtgaagtgat cctcaacgaa tccaatattg
     3781 attctgttct gcttgaagat ggcgacgtca tcaatattcc ggagaaaaca tcgctggtta
     3841 tggttcatgg cgaagtgctg ttcccgaacg cggtgagctg gcagaagggt atgaccaccg
     3901 aggattacat cgagaaatgt ggtggcctga cgcagaaatc gggtaacgcc agaattatcg
     3961 tcattcgtca gaacggtgca gccgtcaacg cagaagatgt ggattcactc aaaccgggcg
     4021 atgagattat ggttctgccg aaatatgaat cgaaaaacat tgaagttact cgtggtattt
     4081 ccaccatcct ctatcagctg gcggtgggtg caaaagtgat tctgtctttg taaggagttg
     4141 aaatgagcaa agctgttatt gtcatcccgg ctcgttatgg ctcatcgcgt ctgccgggta
     4201 agccactgct cgatattgtt ggtaaaccga tgatccagca tgtttacgag cgtgcgttac
     4261 aggtggcggg cgttgcggaa gtttgggtgg caacagacga tccgcgtgtt gaacaggccg
     4321 tgcaggcatt tggcgggaaa gccatcatga cgcgcaacga tcatgaatcc ggcaccgatc
     4381 ggctggtcga ggtgatgcat aaagtcgagg cagatatcta cattaacctg cagggcgacg
     4441 aaccaatgat tcgcccgcgg gatgtagaaa cgctgttaca aggaatgcgt gatgaccccg
     4501 cgttgccagt ggcaacgcta tgccacgcga tttctgccga agaagcgacc gagccaagca
     4561 cggtaaaagt ggtggtaaat acccgccagg atgcgcttta tttcagccgt tcgcctattc
     4621 cgtatccgcg taatgctgaa aaagcgcgct acctgaaaca cgttggtatt tacgcttatc
     4681 gtcgcgatgt gctgcaaaac tacagccagt taccggagtc catgcaggag caggcagaat
     4741 cactggagca gctgcggttg atgagcgcgg ggatcaacat ccgcacattt gaggttgccg
     4801 caaccggtcc gggcgtcgac accccagcat gcctggaaaa agtgcgcgcc ctgatggcac
     4861 aggaactggc tgaaaacgca tgattggcat tttctcgtcc ggtatctggc gtattccgca
     4921 tctggagaaa tttctggcgc aaccgtgcca gaaactttct ctgctgcgcc ctgttccgca
     4981 agaagttgat gctatcgccg tgtggggaca tcgtcccagc gcggcgaaac cagtcgccat
     5041 cgccaaagca gcgggaaaac ccgtcattcg tctggaagat ggatttgtgc gttcgctgga
     5101 tcttggcgtc aatggcgagc cgccgctttc tctggtggtg gatgattgtt gcatttacta
     5161 cgatgccagc aagccttcag cactggagaa actggtacaa gataaagccg gaaatacagc
     5221 tctgataagc caggccagag aagcgatgca caccatcgtg accggggatt tgtcgaaata
     5281 taacctggca cctgcgtttg tggctgatga gtcagaacgt tcagacatcg ttctggttgt
     5341 cgatcagaca tttaatgata tgtcagtgac gtatggcaat gctggcccgc atgagtttgc
     5401 tgccatgctg gaagccgcga tggcggaaaa tcctcaagcc gaaatttggg tgaaggtgca
     5461 tccggatgtc ctggaaggaa agaaaacagg ttatttcgct gatctgcgcg ccacgcaacg
     5521 agtacgtttg attgccgaga atgtcagccc gcagtcgctg ttgcgacacg tttcccgggt
     5581 ttacgtcgtg acatcccaat acggctttga agccttgctg gcaggaaaac cagtaacatg
     5641 tttcggccag ccctggtatg caggctgggg cttaaccgac gatcgccatc cgcagtccgc
     5701 tttgttatct gcccgacgcg gttctgccac gctggaagaa ctttttgccg ctgcatacct
     5761 gcgttactgt cgctatatcg acccgctcac gggggccgca agcgatctat ttaccgtgct
     5821 gcaatggctg caattacagc gcaaccatca gcaacagcgt aatggctatt tatggtcgcc
     5881 aggcttaacg ctgtggaagt cggcaatcct gaaacctttc ttgcgtaccg caacaaacag
     5941 actgagttat tcacgccgtt gtactgcggc gacggcttgc gtggtatggg gtgtaaaggg
     6001 agaacagcaa tggctagccg aagcgcaaca aaaatcactg ccgttatggc gaatggaaga
     6061 tggttttctg cgttcatccg gacttggctc tgacctgctg ccgccgctat cgttggtact
     6121 ggataaacgc gggatctact atgacgccac gcgccccagc gacctggaag tcctgcttaa
     6181 tcacagccag ttaacgctgg cgcagcagat gcgagctgaa aaattacgcc agcgactggt
     6241 tgaaagcaaa ctgagtaagt acaacctggg agccgatttc tctctaccag ccgaagccaa
     6301 agataaaaaa gttatcctgg tgccgggtca ggtagaggac gatgcctcta ttaaaacagg
     6361 cactgtctcg attaagagca accttgagtt attacgcaca gtacgcgagc gtaatccgca
     6421 cgcctacatt gtttataaac cgcacccgga tgtactggtg gggaattgca agggcgatat
     6481 tccggcagaa ctaattgctg aactcgctga ttatcaggca ctggacgcag atattattca
     6541 atgcattcag cgcgcagatg aagtgcacac catgacatca ttgtccgggt ttgaagcgtt
     6601 attacatggc aagcaagttc attgttacgg cctgcccttc tatgccggtt ggggtttaac
     6661 cgttgatgaa catcactgcc cgcgccgcga gcaaaaatta acgttagcag atttgatcta
     6721 tcaggcgttg attgtttatc caacctatat ccacccaaca cggctacaac ctattacggt
     6781 agaagaagct gcggaatatt taatcaagac gccgcgcaag ccgatgttta ttacccgaaa
     6841 aaaagcgggg cgggtaatac gctattaccg caaattaatt atgttctgca aggtcagatt
     6901 tggctaaaca atttcatagt tgactattac gcaaattatt tatgcaaggt aatgcactaa
     6961 ccgttttatt atccggtaaa aaatatctgc tattgcaggg gccgatggga ccttttttca
     7021 atgacgtcgc cgaatggtta gagtcattag gacgtaacgc tgtgaatgtt gtattcaacg
     7081 gtggggatcg tttttactgc cgccatcgac aatacctggc ttactaccaa acgccgaaag
     7141 agttccccgg atggttacgg gatctccacc ggcaatatga ctttgatacc atcctctgct
     7201 ttggtgactg ccgcccattg cacaaagaag caaaacgttg ggcaaagtcg aaagggatcc
     7261 gctttctggc atttgaagaa ggatatttac gtccgcagtt tattactgtt gaagaaggcg
     7321 gagtgaacgc atattcatcg ctaccgcgcg atccggattt ttatcgtaag ttaccagata
     7381 tgcctgcgcc gcacgttgag aacttaaaac cttcaacgat gaaacgtata ggtcatgcga
     7441 tgtggtatta cctgatgggt tggcattacc gtcatgagtt ccctcgctac cgccaccaca
     7501 aatcgttttc cccctggtat gaggctcgtt gctgggttcg tgcatactgg cgcaagcaac
     7561 tttacaaggt aacacagcgt aaggtattgc cgaggttaat gaatgagctg gatcagcgtt
     7621 attatcttgc cgttttgcag gtgtataacg atagccagat tcgtaaccac agcaattata
     7681 acgatgtgcg tgactatatt aatgaagtca tgtactcatt ttcacgtaaa gcgccgaaag
     7741 aaagttattt ggtgatcaaa catcatccga tggatcgtgg tcacagactc tatcgaccat
     7801 taattaaacg gttgagtaag gaatatggct taggtgagcg cgtcatttat gtgcacgatc
     7861 tcccgatgcc ggaattgtta cgccacgcaa aagcggtggt gacaattaac agtacggcgg
     7921 ggatatctgc gctgattcac aacaaaccac tcaaagtgat gggcaatgcc ctgtacgaca
     7981 tcaaaggctt gacgtatcaa gggcatttgc accagttctg gcaggccgat tttaaaccgg
     8041 atatgaaact gtttaagaag tttcgtgggt atttattggt taagacgcag gttaatgcgg
     8101 tttattatgg
//
LOCUS       DACADA010000186         8097 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.188, whole genome shotgun sequence.
ACCESSION   DACADA010000186 DACADA010000000
VERSION     DACADA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.188"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            24..1190
                     /locus_tag="ISB17_004178"
     CDS             24..1190
                     /locus_tag="ISB17_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP1982050.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1233..2549
                     /locus_tag="ISB17_004179"
     CDS             1233..2549
                     /locus_tag="ISB17_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982051.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2653..3303
                     /gene="fabR"
                     /locus_tag="ISB17_004180"
     CDS             2653..3303
                     /gene="fabR"
                     /locus_tag="ISB17_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAP1982052.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3303..3662
                     /locus_tag="ISB17_004181"
     CDS             3303..3662
                     /locus_tag="ISB17_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAP1982053.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(3702..4802)
                     /gene="trmA"
                     /locus_tag="ISB17_004182"
     CDS             complement(3702..4802)
                     /gene="trmA"
                     /locus_tag="ISB17_004182"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAP1982054.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4916..5106
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5171..7015
                     /gene="btuB"
                     /locus_tag="ISB17_004183"
     CDS             5171..7015
                     /gene="btuB"
                     /locus_tag="ISB17_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAP1982055.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6960..7817
                     /gene="murI"
                     /locus_tag="ISB17_004184"
     CDS             6960..7817
                     /gene="murI"
                     /locus_tag="ISB17_004184"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP1982056.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         1852 a         1913 c         2201 g         2131 t
ORIGIN      
        1 ataagtgaat aaaggaatag ccgatgatcg aacactatat tcgtgggttt gaggaagaac
       61 ttcgcgagat ccgccatcag atccacgaaa acccggagct gggattacaa gaatttaaaa
      121 ccagtgcttt ggttgcggaa aagctgcgcc agtggggcta tgaggttgag caagggctgg
      181 cgacaaccgg cgtggtagcc acgttaaaag tgggcgacgg agagaaaagt attggcctgc
      241 gggcggatat ggatgcgttg ccgatttatg aaaatagtgg caagccctgg gccagtaaac
      301 atcctggttt aatgcacgcc tgcgggcatg acgggcatac caccatactg ctgggggccg
      361 cgcgttattt tgccgaaacc cgccgtttta acggcacgtt acgccttatc ttccagccag
      421 cggaagagat gattaacggc ggtgagatca tggttaaaga ggggcttttt gatcgtttcc
      481 cctgcgatgt cattttcggg atgcacaaca tgccgggttt gccggtcggc aagtttttct
      541 tccagcctgg ggcgctgatg gcgtcaatgg atcagttcca tattacggtt cgtggttgtg
      601 gcggacacgg tgcgatcccg cacaaagcca tcgatccagt gctggttgcc gcacatatca
      661 ccaccgcatt acaaagcatt gtgtcgcgca atgtcgatcc gctggaagcg gcggtaatta
      721 ccgtcggcag cattgttgcg ggtgaggcgg ctaacgtcat tcctgacagt gctgagatga
      781 aaatcagtgt ccgctctctt agccgcgata cccgacaact tcttctgacc cgtattcctg
      841 ctcttgcaca aacccaggct gccagctttg gcgctacggc tgaggttacg catgttaacg
      901 gtacaccggt attagtaaat gacgaggaga tggcgcgctt cgcctggcag gtggcgtgca
      961 aaacatttgg tgaagatcgg gctgagtttg gcatcaaacc cctgatgggc agtgaagatt
     1021 tctctttcat gctggaggcc caacccaaag gcggtttcct gttattcggt aatggcgatg
     1081 tcggggaagg ttcctgcatg gtgcataacc ccggttacga ctttaacgat gcaagcctgg
     1141 tcccggcaag cagctactgg ggcgcactgg ttgaagcctg gctgcaataa ataaaacaac
     1201 acacaacaac actttttggc aaggaacatg gaatgacggc gatcgtaaat gaaaccgtga
     1261 gtgtgaagaa aaagcccagc ggcaggcggg tgatctttgc ctcggcgttt ggcaatgcgc
     1321 tcgaattttt tgattttggc gtttacaact tttttgtggt ctatatcagt acgcttttct
     1381 tcccacccag tgccgatcat aacgtcgcgc tgttactggc atttgcaacg tttggtgtga
     1441 gcttttttat gcgtccgctc ggtgggatta tcgttggtgc ctgggccgat cgcttcgggc
     1501 gtaaaccggc gatggtgttc actattgctc tgatgagcct cggtacgctg atgatcggca
     1561 tcgcaccgac gtatgaaacg gcgggctatt ggggaaccgc aacgctggtg ctggcgcgtt
     1621 tgattcaggg cgttgcggca ggtggtgaag tcggggcttc aatgtcgcta ctggtggagt
     1681 cagctccggc aaatcgtcgt ggcttttaca gcagttggtc actggcgact caaggcctgg
     1741 caacaacttt tggcggtgtt gtcgctttag gtttaagcgc atggctaccg ttcgccacag
     1801 gttcagaaac cgtaatggca gaatggggct ggcgtgtgcc gttttttatt ggtgtgttat
     1861 tagcacctat cggttgctgg ttgcgtctga gcctggaaaa tgacgtacct gagcctgtgc
     1921 gcaataaaaa agctgctacc agcgaaagcg cgttttctct gctcctgcaa cataaggcga
     1981 ctatcgttaa tggcgtttta ctcgctattg gtagcacggt cgcaacctat atctccctct
     2041 tttattacgg cacctgggcg gcgaagtatc tggcgatgcc tcaacattat tctcatgcag
     2101 cgatgttact ggcgggcgtt gttacgtttg tgggtgcgct gcttgtgggg atgttgtgcg
     2161 attcggtcgg gcgtaaaaag ctgattttaa tctcccgtgt gatggtgctt ctctgtagct
     2221 ggccgtcatt ctggctgttg gtgaattacc caagccctgg catgttgctg acggtggttt
     2281 tcgtgatggt cagctttacc acgcttggcg gtgtgccagt gatgttgttg atctctgaac
     2341 tgttgccgaa acggattcgg gcattgggtt ttgcgctggt ttatagcatt ggtgtggcaa
     2401 tctttggcgg ttttgctcaa tatttcgcta cgcaatccat cgtgttgctg gatagtctga
     2461 cggctccggc gtggtatctg ggtggaggca cgttgctgtc gatgctggcg ttgttgtacg
     2521 taaaagaacc ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca
     2581 acactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
     2641 gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg
     2701 gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct gagtttgcgt
     2761 gaagtggcac gagaagcagg cattgctccc acctcttttt atcggcattt ccgcgacgta
     2821 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgt
     2881 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
     2941 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
     3001 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
     3061 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
     3121 gcaatggtga cgattgtctt cagtgcgggg gccgaggcgt tggatgtcgg cgtcgagcaa
     3181 cgtcggcaat tagaagagcg actggtactg cagctgcgaa tgatttcgaa aggggcttat
     3241 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
     3301 taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgctagtg gctggcttat
     3361 cgattaatgg tactttcgca gcgctgttta gctccattgt accattttct gtattcccga
     3421 ttatttccct ggtgttgaca gtttactgcc tgcatcaacg ttatcttaat cgcactatgc
     3481 cggtaggctt gccgggtctg gcagctgctt gttttattct cggcgtactg ctgtacagca
     3541 cggtagttcg tgcggaatat ccggatatcg gttccaactt cttcccggca gtactgtccg
     3601 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggac gttgctgagt
     3661 aatcagcatc atgccggatg caggcgcatc cgacatgggt tttacttcgc ggtcagtaat
     3721 acgccgcact ccatatggtg cgtgtagggg aactgatcaa atagagccag acgttcgacc
     3781 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccggatt gcaggagata
     3841 tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
     3901 ggtgggtcga caaaaatggt ttcgcactga taactcttta agtcgattcc ttgcaggcgg
     3961 ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
     4021 tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcggcgac tgacggcttg
     4081 gcgatttcgg tggctaatac ccgatcaaaa ttgcgtgcca gcgctaatga aaagttaccg
     4141 ttgccgcagt atagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat
     4201 tccagcatct gaatattcat cgccgcgttc ggttgggtaa agctgttttc tacctgacgg
     4261 tagatcatct ctttccccgc gatcggcaga cgttcgtcga tgtaatcctg atccagcgcg
     4321 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca
     4381 tcgcgcaggg cttccgcctg ctgacaccac tcatcatcca gctttttatg gtacagcaag
     4441 gaaacaaccg cttgattgct tagcgtagtg aggtaatcaa tctggaacaa cttgtggcgc
     4501 agaatgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca
     4561 ctggcggcgg ggaagctatc cacacggata cgacttttgg tttgttgatc gaaaatgatg
     4621 tggtacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg ataatgactg
     4681 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
     4741 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
     4801 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
     4861 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
     4921 gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
     4981 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
     5041 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc
     5101 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
     5161 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttccgtca cggcattttc
     5221 cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca
     5281 accgcgcagc actgtgcttg cgccaactac tgttgtgacg cgtcaggata tcgaccgctg
     5341 gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtggata tcacccaaaa
     5401 cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt
     5461 ggtgttaatt gatggcgtcc gcctgaattt ggcgggggtg agtggttctg ctgatcttag
     5521 tcagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt
     5581 ttatggttca gatgcaatag gcggggtcgt gaatatcatc acgacgcgcg atcaccccgg
     5641 aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacaca
     5701 gcaacaactg ggggataaga cacgggtaac gctgttgggc gattatgccc atactcatgg
     5761 ttatgatgtt gtcgcctatg gtaataccgg aacgcaagcg cagccagata acgatggttt
     5821 tttaagtaaa acgctttatg gcgcgctgga gcataatttt actgatgtct ggagcggctt
     5881 tgtgcgcggc tatggctatg ataatcgtac caattatgac gcatattatt ctcccggctc
     5941 gccactggtc gatacccgta aactctatag ccaaagttgg gatgccggac tgcgctataa
     6001 cggtgaactg attaaatcac aactgattac cagctacagc catagcaaag attacaacta
     6061 cgatccgcat tatggtcgtt atgattcatc cgcgacgctc gatgagatga aacagtacac
     6121 cgtccagtgg gcaaacaaca tcatcattgg ccacggtaat attggtgcgg gtgttgactg
     6181 gcagaagcag agcacggcac cgggcacagc ttatgttgag gatggatatg atcaacgtaa
     6241 taccggcatc tatctgaccg ggctgcaaca agttggcgat tttacctttg aaggcgcagc
     6301 acgtagcgac gataactcac agtttggtcg tcatggcacc tggcaaacca gcgccggttg
     6361 ggaatttatc gaaggttatc gctttattgc ttcctacggg acatcttata aggcaccaaa
     6421 tcttggacaa ctgtatggtt tctacggaaa tccgaatctc gaccctgaga aaagcaaaca
     6481 gtgggaaggc gcgtttgaag gcttaactgc tggggtgaac tggcgtattt ccggatatcg
     6541 taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt acaacgaagg
     6601 gaaagcgcgg attaagggtg tcgaggcgac cgccaatttt gataccggac cactgacgca
     6661 tactgtgagt tatgattatg ttgatgcgcg caatgcgatt actgacacgc cgttgttacg
     6721 ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
     6781 gattacttat cagtacttag gttctcgtta tgataaggat tactcctctt atccgtatca
     6841 aacagtgaaa atgggcgggg tgagcttgtg ggatcttgct gttgcatatc cggtcacctc
     6901 tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattacg agacagtcta
     6961 tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac
     7021 gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc
     7081 ggcatctctt gccggatctc cattacattt atgctttcga taacgtcgct ttcccgtatg
     7141 gcgaaaaaag cgaagtgttt attgttgagc gagtggtgga aattgtcacc gcagtgcaag
     7201 aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc gtttcacttc
     7261 ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg
     7321 ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga actgttaaac
     7381 gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg
     7441 gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat gtttctctgg
     7501 atgcgctaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca gatacagttg
     7561 tattgggttg cacccatttc cctctactac aagaagaact attacaagtg ctgccagagg
     7621 gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagagc
     7681 atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc attttgtatg gcaatgacgc
     7741 cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac
     7801 tggcagtttt agattgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc
     7861 tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa
     7921 cacgccgccg ggtcagcggg gttctcctga gaatctcaac agaggaaagc aaaaaaatgc
     7981 ttgactctgt agcgggaaag cgtattatgt ataccccgcg ccgctgagaa aagcgaagcg
     8041 gcactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggatt
//
LOCUS       DACADA010000187         8017 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.189, whole genome shotgun sequence.
ACCESSION   DACADA010000187 DACADA010000000
VERSION     DACADA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.189"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(65..943)
                     /gene="glxR"
                     /locus_tag="ISB17_004185"
     CDS             complement(65..943)
                     /gene="glxR"
                     /locus_tag="ISB17_004185"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP1982057.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1043..1819)
                     /gene="hyi"
                     /locus_tag="ISB17_004186"
     CDS             complement(1043..1819)
                     /gene="hyi"
                     /locus_tag="ISB17_004186"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAP1982058.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1832..3613)
                     /gene="gcl"
                     /locus_tag="ISB17_004187"
     CDS             complement(1832..3613)
                     /gene="gcl"
                     /locus_tag="ISB17_004187"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAP1982059.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3703..4518)
                     /gene="allR"
                     /locus_tag="ISB17_004188"
     CDS             complement(3703..4518)
                     /gene="allR"
                     /locus_tag="ISB17_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAP1982060.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(4596..5078)
                     /gene="allA"
                     /locus_tag="ISB17_004189"
     CDS             complement(4596..5078)
                     /gene="allA"
                     /locus_tag="ISB17_004189"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAP1982061.1"
                     /translation="MKLQVLPLSQKAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5308..6234
                     /gene="allS"
                     /locus_tag="ISB17_004190"
     CDS             5308..6234
                     /gene="allS"
                     /locus_tag="ISB17_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAP1982062.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6303..7409
                     /gene="mnmH"
                     /locus_tag="ISB17_004191"
     CDS             6303..7409
                     /gene="mnmH"
                     /locus_tag="ISB17_004191"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAP1982063.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
     gene            complement(7461..>8017)
                     /locus_tag="ISB17_004192"
     CDS             complement(7461..>8017)
                     /locus_tag="ISB17_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HAP1982064.1"
                     /translation="YFNFFYPNSLPAEVTYVELLDTDGIYYQFRTLASTNASPGSVGQ
                     WSEKVSGMSSVFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGDVWKLMLTPYSPPGG
                     GSEKYYLRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAY
                     KHGYSYPESTKNFIKDKKYPYGNW"
BASE COUNT         2034 a         2103 c         1890 g         1990 t
ORIGIN      
        1 gctttctttt tttaatattg ctggccgatt cattttctga tatcggccat ttttattgcg
       61 ggtatcaggc cagtttatgg ttagccatta attccagcgc ctgcactaat gcagagtgat
      121 ccaactggct gccaccgttt gccgcacagg tattaaacaa ttcctggcag gtcgcggtgt
      181 ttggcaggtt cagcgccagt gctttcgcac tttgcagtgc caggttgaga tctttctggt
      241 gcagcgcaat tttgaaacct ggattaaagg tgcgtttaat catgcgctca ccgtgaactt
      301 ccagaatacg tgaggaagca aagccgccca tcagcgcctg gcgcacacgt accgggtccg
      361 caccggcttt tgaagcaaac aacaaggctt cagaaaccgc ttcaatattg agcgccacga
      421 taatctgatt tgccactttg caggtttgac cgtcgccgtt accgcccacg agagtgatat
      481 ttttaccgag cagttcaaac agcggtttta cacgttcaaa tactgcttca tcaccgccaa
      541 ccataatcga taacgtccct tcacgcgcgc cgatttcacc gccggagact ggcgcatcga
      601 gataatcgcc gcccagttca ttcacctgac gagcgaaacg tttagtttca atcggggaaa
      661 tggagctcat atcaacaatg gttttgccct tcagcgaggc tttggtacaa ccattttcac
      721 caaacagaac gtcttcaacc tgtggcgtat ccggcaccat aataaaaatg atgtccgatg
      781 catctgttac ctggcgcgca gtttcaacac taacggcgcc cagtgacagt aattcatcag
      841 caaccggacc aatggttgtg acatgcagtt gatgaccggc acgcgccaga ttaatggcca
      901 tcggtgtacc cataatgcct aagccaataa atcccagttt cataattaac ctcttttaaa
      961 tttcgctttt cctgaattca gacaacacga tcccctgcca acttagcggc ctaaaaaagc
     1021 attgtctgaa tagcgttacg ttttaacggt acgggtccat ccagcgtaaa ccggcttccg
     1081 tggtggtttg gggtttatat tcacagccaa cccaaccgtt gtagtcagaa ttttcaatta
     1141 ccgtaaagag atattcgtaa ttaatttctc cggttcccgg ttcgccgcga tgcggattat
     1201 cagcaatttg caggtgacca attttatcgg cccactgagt catggtattg gttaattcac
     1261 cttccatccg ctgcatgtga taaatgtcat actgaatttt caaattgcag caaccaacat
     1321 cgtcaatcag tttcagtgcc tgccgggttc cggtgagatg aaaaccagga atatcaaaat
     1381 ggttaatagg ttcaatcagt aataaaatat cttctttcat cagcatattc gcagcataac
     1441 gcaggttttc tacaagcgtt gtgtgaatct gttcactgct gaaaccagtc ggtgttttac
     1501 cgaccagaca gttaattttt ttattaccca gcgcacgagc ataacgaatc gctgctgcta
     1561 cgccatcccg aaactcttct tcacggccag gaatacaggc aataccccgc tcgcccgccg
     1621 cccagtcacc tgccggtaaa ttgtgcagcg tatgttcaag tttattactc gccagcacct
     1681 gttttaattc ttcaatgtcg tagtcataag ggaacataaa ttcaacgccg cgaaaaccac
     1741 actgcgcagc tttctcaaaa cgggcgagaa aatcatattc accaaataac atcgataaat
     1801 tagcagagaa acgtaacatt atttatctcc cttattcata gtgcatgaaa caggtttcag
     1861 tcggtgcatc cgctgcgtta tcggcgatat cttcaaattc catgacgtta tccagttcac
     1921 tgcccatcga aatattagtc acacgctcga gaataacttc cacgactacc ggtacccgat
     1981 attgcgccat taagattttc gcctgttcaa aggctggtgc aatatcttcc ggtttgaaaa
     2041 cgcgaattgc tttacaacct aaaccttccg ctacttttac gtggtcgacg ccataaccgt
     2101 tcacttcgct ggagttaata ttttcgaacg cgagctgtac gcagtaatcc atatcaaagg
     2161 cacgttgcga ctggcgaatc agacccagat aggcgttatt gaccagtaca tggatgtacg
     2221 gaatgttgaa ctgtgcgcca acggctaatt cttcaatcag gaactggaag tcgaagtcac
     2281 cggaaatcgc caccacattg cgctctggat cagcggcaca aacccccagc gccgccggaa
     2341 tcgtccagcc cagcgggcct gcctgcccac agttgatcca gtggcggtct ttaaagacat
     2401 gcagcatttg tgccgcagcg atttgtgaca ggccaatggt ggtgacataa caaacatcgc
     2461 ggccaaaggc tttgttcatc tcttcataca cgcgctgcgg tttcaccggc acgttgtcga
     2521 agtgggtttt gcgcagcaaa gtgcgtttgc gctgctggca gtcggcgacc cactctttac
     2581 gacacggcag acgaccggct ttttgcatct cctgcgccac ttcaaccagc agcgtcagcg
     2641 ccgctttagc atcagagaca atgccaagat ccggacacag cacgcggcca atttgcgtcg
     2701 gctcaatatc gatatgaacg attttgcgcc cttcggtgta tttctctacc gaaccggtat
     2761 gacggttagc aaaacggtta ccgataccaa acaccatgtc ggacgccagc aacgttgcat
     2821 taccgtaacg atgcgcggtt tgcaggccca ccatcccggc catcagttca tgatcgtccg
     2881 ggatacagcc ccagcccatc agcgttggga tcaccggaat gctggtcagt tcagcaaact
     2941 gttgtaacag tacagctgcg tcagcattaa ttaccccgcc tccggcaaca atcaccggac
     3001 gttcggcctg gattaacatt tctacagctt tttcgatctg catacggctg gcagcaggtt
     3061 tgtagaccgg cagcggttcg tacatatcag gatcaaactc gatttccgca acctgtacgt
     3121 cgaacggtaa atccaccagt accggacccg gtcgaccaga acgcatcagg tgaaacgcct
     3181 gttgcagcac acgaggcacc agcgccgctt cacgaactgt aaccgccatt ttgctgaccg
     3241 gtttagcaat tgcttcaata tcaacggcct ggaaatcttc tttatgcaga cgggcgcgcg
     3301 gagcctggcc ggtaatacac agaataggaa tggaatcagc agatgcggaa tagagcgcgg
     3361 tgatcatgtc cgtgcccgca ggaccggaag tccccagaca tacgccgata ttccctgccg
     3421 ttgcgcgggt ataaccttcc gccatgtgcg aagcaccttc cacatgacgc gccagaatgt
     3481 gacgaatacc gccgtgctta cgcatcgctg agtagaacgg attgattgca gctcccggaa
     3541 cgccgaaggc ggtggtgata ccttctttct ccagcacata cattgccgcg tcaacggctc
     3601 tcatttttgc cattttattc ctacctctat taattggaaa aattttccaa ctttcaaatt
     3661 ttgtcaaaaa aacccgctcc gcagagcggg atacgacaga cactatagat gtgctttcag
     3721 tcccaacgcc gtgctgatat cgcgggcggt gtctctgacc agctcaccct gactgacaaa
     3781 acgatcttct gtcagtcttg atgaaggccc ggagatggag atagcggcaa caacactacc
     3841 tacatcatcg taaattgctg aagctatgca attcaggccg acaacatgct cttctttatc
     3901 tacggtatag cccagttcac gcgcttgttc caggtccttc agcaaggtgg gcatatccac
     3961 aagtgtcgtt ggcgtaaact gctgcaaacc ggtttgcaga atgatgctca tcaactcttc
     4021 ttccgccagc ggataaagca gcgccttgcc cgctccggaa gcatgcagcg gcagacgact
     4081 gcccagcggc gcacacatcc tgaccatcga tttacactct aactgaccaa ttaataccgc
     4141 ttcattgccg ttacggatcg cgacattgac cgtttcgccg gaaagtaaca tcaggcggcg
     4201 cataaacggc ccggcgacgg agaggacatc gcggttatgg atgtacgctg caccgacgtt
     4261 aaagacacct aatcctatat gccaccagcc taactgattg tcctgataga caaaatccgc
     4321 tgcctgtaaa accttcagca agcgaaaggt cgtggagagc ggcaaatcca gattgagaga
     4381 gatatcgcta accgacgaac ttcccccact tttttccaga tattgcagaa tcgcaattcc
     4441 ccgctctaac gcctgtgcgc ccttctgtgc tacaggctcc gcctgtcctg gcctgccgcg
     4501 ccgtctaact tccgtcatac ctacgttaac tccgacctac aaatgagcat aaacaatgaa
     4561 caattcgttt atcttatgca agtcggttgc aggcgtcacg caaaacagag ttcctgttca
     4621 ggaatacttt caacatcaca gttgtcactg ccgccgcgat cgatggtcag aaaatcggtg
     4681 acgcgctgcc aggcgaaaag tgggtgatgc cagacgttac gatggtaatt cactccctgt
     4741 tcgccattgg taataaacgc ccgcagggtt gacaggtctg gtttgtcgtc acctaacgcc
     4801 accaccacca caaacacttc acctttcatc gggataaacg cctgtgtacc cagcggatga
     4861 cgttcgagtt cgtgaatggt cagcggcaga ttcgccggtt gcgcgcggtt aatgctgata
     4921 agcgtacggt cttgctcaag aatctcaacc agcgccaaat cgtggtaacg ctccaccagt
     4981 ccattgttaa tatggaaaaa atcccgtttc tgcgtttcga ttacgtcgcc ataagcacta
     5041 aaggctttct gacttaacgg taatacctga agtttcatct ttttgtcctt gtaacataat
     5101 tccgttcaac caccgccgac cggtcagctt ggtttcaata acaggcaatc taaaacctta
     5161 agagaacaac atcaacacaa aagttggaat atttttccaa aactagattt agtccacaaa
     5221 ttgaacaggg ttatcatgta aaaatactga taaatcagag gataagaaaa tcacgcaact
     5281 tgcggattta aaaggggata cctgaagatg ttcgatccag aaaccttgcg gactttcatt
     5341 gcggttgctg aaacaggtag tttttcaaaa gcggcagaac gattatgtaa aaccacggcg
     5401 acgatcagtt atcgcattaa acttctggaa gagaataccg gagtggcgct gtttttccgc
     5461 acgactcgca gcgtgacgtt gacggcggct ggcgagcatc tactttgtca ggccagagac
     5521 tggctgagct ggctggaaag tatgcccagc gagttacaac aggtgaatga tggcgtggaa
     5581 cgccaggtga atatcgtcat caacaacctg ctctataacc cccaggccgt tgccaggctg
     5641 ctggcgtggc taaatgaacg ttacccgttt acccaatttc acatctcccg acaaatctat
     5701 atgggcgtct gggactcgct attgtacgaa ggtttttcac tggctatcgg cgtcacggga
     5761 accgaggcgc tggcaaatac ctttagtctt gatcccttag gatcggtgca atggcgcttt
     5821 gtcatggcgg cggatcatcc gctggcgaac gttgaagagc cgctaacaga agcacagttg
     5881 cggcgctttc cggcggtcaa tattgaagac agcgcccgca ccttaaccaa acgcgtcgcc
     5941 tggcgattgc cggggcaaaa agagattatt gttcccgata tggaaacgaa aatcgccgcc
     6001 catctggcgg gcgttggcat tggttttttg ccaaaatcac tttgccagtc aatgctcgat
     6061 aatcaacaac tggtcagccg ggtaatccca acgatgcgcc ctccttcgcc attgagtctg
     6121 gcatggcgca aatttggcag cggcaaagcg gtagaagata ttgtgacctt gtttacccag
     6181 cgcaggccgg aaatcagcgg atttttagaa attttcggca acccacgcag ttaaaaatca
     6241 agtttccttg cgcgttggcg cgtaaaatag cgcgcaagct gatttgaggt tcacttttta
     6301 ctatgcaaga gagacacacg gaacaggact atcgtgccct gctgattgct gatacgccca
     6361 ttattgatgt tcgcgccccc atcgagtttg agcaaggcgc aatgcccgcc gctatcaacc
     6421 tgccgttaat gaataacgat gaacgcgccg ccgttggcat ctgctataaa cagcaagggt
     6481 cagacgcggc gctggcactg gggcataaac tggtggcggg tgaaattcgt cagcagcgca
     6541 tggacgcctg gcgggcagcg tgcctgcaaa atccgcacgg tattctctgc tgcgcccgtg
     6601 gcggtcagcg ctcacatatt gtgcaaagct ggttgcatga cgcggggatt gattatccgc
     6661 tggtggaagg cggttataag gcactgcgcc agaccgcgat tcaggcgact attgaactgg
     6721 cacaaaaacc gatagtgctg attggcggtt gtaccggcag cggtaaaacg ctgttagtgc
     6781 agcaacagcc gaacggtgtt gatctggaag ggttggcgcg tcatcgcggt tcggcgtttg
     6841 gtcgcacctt acaaccacaa cttagccagg cgagttttga aaacatgctg gctgccgaaa
     6901 tgctaaaaac cgacgcccat caggatttgc gcctgtgggt gctggaagat gaaagccgga
     6961 tgatcggttc gaatcacctg ccggaatgtc tgcgcgaacg aatgactcag gcgacgattg
     7021 cggtggtaga agatccgttt gagatccgtc ttgaacgcct gaacgaagaa tatttcttgc
     7081 gtatgcatca tgattttacc cacgcttatg gcgacgaaca gggctggcag gagtattgcg
     7141 aatacctgca tcacgggctt tcggcgatta aacgtcggct gggactacaa cgctataacg
     7201 aactggctgc aaggctggat gcggcgctga caacgcaact caccaccggc agcaccgacg
     7261 gtcatctggc ctggctggtg ccgttacttg aagaatatta cgacccgatg tatcgctatc
     7321 agcttgagaa aaaagcggaa aaagttgtct ttcgcggtga gtgggcggaa gtggcggaat
     7381 gggttaagac ttatgcctgt ggttattaac ccacaaaaat gcttttccgt tcctctttaa
     7441 taaattgtgc aactggttaa ctaccagttg ccatagggat attttttatc tttgataaag
     7501 ttttttgtac tttccggata agaataccca tgtttatagg cacttcggcc gttacacatt
     7561 tccatttttt cacctgaaac cgtttctatc agaatctgct tatccgtcag gcgaatattg
     7621 ggcttgtcag gtttctccag ccacactcct attttccctt ccggagccag cccgattaac
     7681 agataccgca gataatactt ttcactgcca ccgcctggtg gtgaataagg tgtgagcatc
     7741 aatttccaga cgtctcccgc cagtgttatc caggtttcat acactttttt atcaatcact
     7801 gaatcccagc aaaaatggat agacattggt gggtttttgg ctttattaaa aactgacgac
     7861 atccctgata ctttctcgct ccattgtcca acacttcctg gactggcatt agtactcgcc
     7921 agagttctga actgataata gatgccatcg gtatctaata actccacata ggtaacttct
     7981 gctggtaggg aattagggta aaaaaaattg aaatacc
//
LOCUS       DACADA010000188         7977 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.190, whole genome shotgun sequence.
ACCESSION   DACADA010000188 DACADA010000000
VERSION     DACADA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.190"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            179..1579
                     /locus_tag="ISB17_004193"
     CDS             179..1579
                     /locus_tag="ISB17_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982065.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLVVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            1590..3563
                     /locus_tag="ISB17_004194"
     CDS             1590..3563
                     /locus_tag="ISB17_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HAP1982066.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQVDN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(3740..5278)
                     /locus_tag="ISB17_004195"
     CDS             complement(3740..5278)
                     /locus_tag="ISB17_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP1982067.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSYGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(5386..6681)
                     /locus_tag="ISB17_004196"
     CDS             complement(5386..6681)
                     /locus_tag="ISB17_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAP1982068.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(6811..7962)
                     /gene="yiaY"
                     /locus_tag="ISB17_004197"
     CDS             complement(6811..7962)
                     /gene="yiaY"
                     /locus_tag="ISB17_004197"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAP1982069.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1844 a         2139 c         2036 g         1958 t
ORIGIN      
        1 tttgattatt aatcaaaaac attaatttca cctgatccat ttcacacttt taaattcagg
       61 tcggaatcgt cacctttttt tactcttttc tcgccttgtt gatattcccg ttcacggcag
      121 aattaatcag gtcattgcaa taaaactaaa aattgctccc tattcacgag gaatatctat
      181 gacctctaca ccgattacca ccgctgatat cgctaaaggt aaaattgacg atgcgttatc
      241 tgtacgggaa aaaataggct acggcctggg tgacgcaggc ggcaccgtaa taacttgcct
      301 gatcatgaac tttctcacct ttttctacac cgacgttttt ggcttaactc cggcgctggt
      361 tggcacgctg tttattgcgc tacgcgtgtt tgatgccatc tccgacccgg tgatgggcgt
      421 cattgccgac cggacgcaaa gtcgctgggg gcgctttcgt ccgtggcagt tatggattgc
      481 cattcccatc ggcattatcg gcatcctgac attcaccgtg ccagatgcca gcatgggagt
      541 aaaaatcgcc tgggcgttcg gtacttacct gctcctttca gtcggttata cagccatcaa
      601 cgtaccgtac tgcgcgctga tcaacaccat gaccacccgc cacaatgaag tgatctcctg
      661 ccagtcctgg cgattcgttc tctgcggcgt agcgggattt ctggtttcgg taggcttacc
      721 gtggatggta gatctcttcg gtcagggcaa cgctgcacgc ggctatcaac ttggcgtcgg
      781 cgtattgtgc gccattgccg tggtgatgtt cctgtgctgt ttcttctggg ttcgtgaacg
      841 ggtgccactc tccacaatgg ggaaatttac cctgcgcgaa catcttgccg ggctgcggaa
      901 caacgaccaa ctgctgctga tgctggtcat gtctttcctg ctgattaacg tctttaatat
      961 tcgcggcggt gggtatatgt acttcattac ctacgtctta caaggcagta cgggctacac
     1021 gtcgctgttc ttcaccatgg tcaccttcgc ctccattatc ggctcggtga ttgtcagccc
     1081 attaacgcgg cgtttcgata ccgtcaaaat ttactactac accaacctgc tcctcgctgc
     1141 actggcggtg ttgatgtggt tcctgccctc cagcccggct tatcaaacgc tgtggctggt
     1201 ggtgatcctc ggtaatggcg tgattcttgg cttcacattg ccactgcact tctcattgat
     1261 ggcctttgcc gatgactacg gcgagtggaa aacccgcgta cgttcttccg gcatgaactt
     1321 cgccttcaat ctgtttttca tcaagctggc ctgggcctcc agcgccggga tcatcagcct
     1381 gctgtttatt tttgtcgcct accagccagg cgtggaaaac cagaccgcca gttcgcttgg
     1441 cgggatcgcg gcgatggaaa cattgctgcc tgcgctgttc cacctgctgc tggcgatggc
     1501 gatccgcttt tgcaaactca ataaccctat gatgtcacgc attgctagcg acctgcgtca
     1561 gcgtcatgta cagccttaag gagcacaaga tgaacatttc ggaagtcgat ctgcataaac
     1621 tgacggtcag cgatccgttc ctcggtcagt accaacaact ggtccgcgac gtggtgattc
     1681 cttatcagtg ggatgccttg aacgatcgta tcccagaagc ggaacccagc catgcgattg
     1741 aaaactttcg cattgccgcc ggacttcagg agggtgaatt ttacgggatg gtgtttcagg
     1801 acagcgacgt cgccaaatgg ctggaagcgg tagcctggtc gctgtgccag aagccggacg
     1861 ccgaactgga aaaaaccgcc gacgaggtga ttgaactgat cgcttccgcc cagtgcgaag
     1921 atggctatct caatacttac tttacggtaa aagcgcccga agaacgctgg agcaatctgg
     1981 cggagtgtca tgaactttac tgcgccggtc atctgattga agccggagtc gccttcttcc
     2041 aggctacggg caagcggcgc ttgctggaag tggtttgccg tctgaccgat catatcgaca
     2101 gcgtatttgg tccagatgaa agtaagttac atggttatcc tggtcacccg gaaattgaac
     2161 tggcactaat gcgcctgtat gaagtgaccg aagagccgcg ctacctggcg ctgacgaact
     2221 attttgtcga acagcgtggt gcgcaaccgc actattacga ccaggaatat gaaaagcgcg
     2281 ggcagacctc gcactggcac acctacggcc cggcgtggat ggtgaaagac aaagcctaca
     2341 gccaggcaca tttgcccatc gcacagcagc aaaccgccat tggtcacgcg gtacgttttg
     2401 tctacctgat gaccggcgtc gcgcatctcg cgcgtttaag tcacgatgaa agcaagcgtc
     2461 aggactgcct gcggctgtgg aacaatatgg cccagcgtca gttatatatt accggcggca
     2521 ttggctcaca gagcagcggt gaagcgttca gcagcgatta cgatctgccg aatgacacgg
     2581 tatacgctga aagttgcgcc tccatcggcc tgatgatgtt tgcccgacga atgctggaaa
     2641 tggaaggcga cagtcaatat gccgatgtga tggagcgcgc actgtacaac accgtgctcg
     2701 gcggcatggc gctggatggc aaacatttct tctatgtgaa tccgctggaa gtacatccaa
     2761 aatcgctgaa attcaaccat atctacgatc acgttaagcc gatccgccag cgttggtttg
     2821 gctgcgcttg ttgtccgcca aatatcgccc gcgtgctaac ctcgattggt cattatctct
     2881 acacgccgcg tgaagatgcg ttgtatatca acatctacgc aggaaacagc atggaggtgc
     2941 cggtagaaaa tggcacgctg cgcctgcggg ttagcggaaa ctatccgtgg caggaacagg
     3001 tgacgattgc ggttgaatca ccccagccgg tgcgtcatac gctggcttta cgtctaccgg
     3061 actggtgcat acagccgcag atcatattga atggggaaga ggtcgagcag gatattcgta
     3121 aagggtattt gcacattacc cgtgaatggc aggagggcga cacgctgaat ctgactttgc
     3181 caatgccggt acgtcgcgtt tacggtaacc cgctggtgcg tcacgtcgcc ggaaaagtgg
     3241 cgattcagcg cggcccgctg gtgtattgtc tggaacaggt cgacaacggc gagtcactgc
     3301 ataatctgtg gctgcccgcc gatgcgccat ttacgacatt tgaaggtaat ggattgttcc
     3361 gccataagat cttaatccag gcaccgggtt accggtatga acagagcaat ccagagcagc
     3421 aaccgctgtg gcattacgac agcgcgccag ccaaacgcca gccgcaaatt ctgacgttta
     3481 tcccgtggtt tagctgggcc aaccggggtg aaggcgaaat gcggatctgg gtgaatgagg
     3541 aaaccagtgc catccgtagg taggataagg cgctcgcgcc gcatccaacg catggtgcac
     3601 attgccggat gcggcgtgaa cgctttatcc gacctacaaa agcatgcaaa ttcaatatat
     3661 tgcatgagct atgtaggcat gataagacgc gaaagcgtcg catcaggcaa tgaatacccg
     3721 atgcgaccag cttcttacat cagaacagcc ccaacggttt atccgagtag ctcaccagca
     3781 ggcacttggt ttgctggtaa tgctcaagca tcattttgtg ggtttcacgg ccaatgcctg
     3841 attgtttgta gccgccaaac gccgcatgtg ccgggtaagc gtgataacag ttggtccaca
     3901 cgcgccctgc ctgaatgccg cgccccatct tataggccag attaccgttg cggctccaga
     3961 cgcccgcgcc cagaccatat tgcgtatcgt tcgccagctc cagcgcttct tccatcgttt
     4021 tgaaggtggt taccgccagc accgggccaa aaatctcctc ctggaacacg cgcatattgt
     4081 tctgaccaaa cagaatcgtc ggttcgaggt agtagccgtc tttcagttcg ccttccagca
     4141 gcttacgtcg cccgccggtg agcacatcag cgccctcttt tttaccgata tcaatgtagt
     4201 tgaggatggt ttccagttgc ccgtaagaaa cctgcgcgcc catttgcgtc acgctgtcga
     4261 gcgggttacc gctgcgaatg ctttcgacac ggcggatagc gcgttccata aagcgttcgt
     4321 agatagactc ctgcactaaa gcacggcttg gacaggtgca aacttcgccc tggttaaagg
     4381 caaacagtgc aaagccttcc agcgctttat cgaaaaaggc atcttcttca tccatcacat
     4441 cagcaaagaa gatatttggc gatttgccgc ccagctccag cgtcaccgga ataatgtttt
     4501 gcgtggcgta ttgcataatt tgttggccca cttccgttga gccggtaaac gccactttgg
     4561 cgatgcgttt cgaggtcgcc agatattcgc caatttcccc acctgcgcca ttgaccacgt
     4621 tcaccacccc tggcggcagt aaatcaccga cgatttccat tagcagcagt acagaaagcg
     4681 gagtaagacg tgcgggtttc agcaccacac agttgcccgc cgccagcgcg ggggccattt
     4741 tccagctcgc catcagcagc gggaagttcc acgggataat ctgccccacc acgcctaacg
     4801 gttcatggaa atgataggcc acggtttcgc tatccacttc actgatccca ccttcctgcg
     4861 cccgaataca cgaggcgaaa tagcgaaaat gatcaatcgc cagcggcacg tcggcagcac
     4921 tggtttcgcg aatgggtttg ccgttatccc aggtttctgc cgtcgctaaa agttcaaggt
     4981 tttgttccat tcgatcggca atcttaaaca gaatcgccgc gcgatcctgc accgaggtgt
     5041 gcgcccattt atctttcact ttatgcgcag catccagcgc cagatcgatg tctcgtttgc
     5101 ccgaagacgc cacttcgcac agtagctgcc cggtcaccgg cgtcaggttc tggtaatact
     5161 cgccatcggc aggcgctacc cattcgccac cgataaagtt gtcgtagcgg gcttttaatt
     5221 tgagggggaa accatactcg ccgggcttaa tctgtgctga agggggatta ttggtcatga
     5281 tcgtctcctt gcggtgtgag gtataacaag ggtagacgtg actggcgaaa tcttcgccag
     5341 tcggtaacag ctttacgacg gctatcacga atttacgggc aaaagctaca gcgggtagtg
     5401 tgcgacgtac actgcttgct ggttattaat tcttttctct tgcgcgaatc cttctttaac
     5461 aattgtccgt aaatcagctc gagccgtatt atcagagacg cccaagctcg cgctaatttc
     5521 agcaactgtg tactctttcc ccggattggc gagcatcaca ttcaggagcg ttacctggcg
     5581 cggattcagg cgtttaagca caccagagct aaacagcaca ctatcgagtt ttgcgcttcg
     5641 ttgtgtaatt tcatcgatat gctgctgcca gttttgcaga gcacgtttaa tgacacccgc
     5701 ctgatattca aggaaataag tcagatccat accatctgat tcagtgtact ggtagctggc
     5761 ggcatatttg accggtgcag catgaagcaa acggctgatg gaaatatatt taaagacatc
     5821 gtagcccgat ttcagcatat accagtaaaa cagcgcccga ctggtgcgcc cgttgccatc
     5881 ccgaaacgga tgttcatggg ccagcataaa atgcagaatg caggcacgga ccaacggatg
     5941 aatataccct tcatggctgt ttaaccactg gcaaactttt tccagccgtt ccggtaacaa
     6001 tgccgcagcg ggcggttgat gcactatatt gccatcatag tcggcgatca caacatcatc
     6061 tgtttcacga aattctcccg gacgatattt cgcgtcattg attccaccca taccaatggc
     6121 atgtaactgg cgaataagcg caggtgtcag cggttctgcg agtaagtgcg gaatttccgc
     6181 catcagccgg gcatttcccg caatcatatg ctcgtcttca gtgcgtggcg accgccctgt
     6241 ttccagcatt tttctcgcca ccagcgttgt agtattcgca ccttccagct gggagctggt
     6301 gatcggctct tccaggcgta actggcttaa ctcggctccg gccccgcgta accgttcagt
     6361 ctgatctttc agcgccagcg cggtagcacg tttgtcgaca agctcgcacg cttctgcaat
     6421 caccggggta atgttgaatc cggcctgttc acccgcttca ttgcaataat taattcgttg
     6481 gatcgccgcg ttgcgcgcca gacgggtaag cgtccacgcc acatcgacgt tttctccttt
     6541 tttgatgcgg tactgcaact catcaaaagg cagataacgt cctttttcat cggtaacccg
     6601 gttaaagcgc aggtgggcca ggagttcctc acccgataag tgtgataaag gaataagtcc
     6661 gggaggtgat ttgatagcca tgtgcttatc tcaaaaaagc gcaatattga gatagtgtat
     6721 agcaggaaag agaaactatc tcaaaaaaag caagttttga gatagttgaa aaagcggctg
     6781 acaactcgcc agccgttgtg ggaatgatga ttacatcgct gcgcgataaa tcgccacaat
     6841 ttcttcgtga gttgcctgga tcgggttagt aaagccgcag gcatctttca gggcattagt
     6901 cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg ccgggatatc
     6961 cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt ccgcgtcgtt
     7021 tttacctgtc acgttcacgc ccattgcagc ggcacaatca cgcagacgtg cagcggcgac
     7081 tttgctgttg aatacctgaa catgcggcag caaaacggcg ttacatacac cgtgtggcag
     7141 gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag aagcattatt
     7201 gaacgccata ccggcgagga actgggcata agccattgct tcacgcgctt tcgcattact
     7261 gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt tcagtgcaca
     7321 agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt gcgttaaggc
     7381 atccataccc gttgcggcgg tcagtgactt cggcataccg atcatcagag aggagtcatt
     7441 gacagaaagc agcggagtga catgtttatc cacaatcgcc attttgatgt gacgcgcttc
     7501 gtcagtgatg atgcaaaaac gggtcatttc agaggccgta cccgccgtgg tgttgatggc
     7561 gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcataatcgc ggatgtcacc
     7621 gccgttggct gcaaccagcg caataccttt tgcgcagtcg tgtggagaac caccgcctaa
     7681 ggaaatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcag cgacgttttc
     7741 cgtggtggga ttaggttggg tgccatcata aataacgcta aaaatattgc gttcttccag
     7801 tgctttttgc acatcacccg ccatacccaa tttcgttaac atattgtcgg tgacaattaa
     7861 ggtacgggta aatccataat ctgccatcat attcattgca tcattcaatg aatcagcacc
     7921 aataacattc acggaaggaa taaaaaaggt tgaagctgcc ataacacact cctgaaa
//
LOCUS       DACADA010000189         7865 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.191, whole genome shotgun sequence.
ACCESSION   DACADA010000189 DACADA010000000
VERSION     DACADA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.191"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            72..1061
                     /gene="tdcB"
                     /locus_tag="ISB17_004198"
     CDS             72..1061
                     /gene="tdcB"
                     /locus_tag="ISB17_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAP1982070.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            1083..2414
                     /gene="tdcC"
                     /locus_tag="ISB17_004199"
     CDS             1083..2414
                     /gene="tdcC"
                     /locus_tag="ISB17_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAP1982071.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            2440..3648
                     /gene="tdcD"
                     /locus_tag="ISB17_004200"
     CDS             2440..3648
                     /gene="tdcD"
                     /locus_tag="ISB17_004200"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAP1982072.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            3682..5976
                     /gene="tdcE"
                     /locus_tag="ISB17_004201"
     CDS             3682..5976
                     /gene="tdcE"
                     /locus_tag="ISB17_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAP1982073.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            5990..6379
                     /locus_tag="ISB17_004202"
     CDS             5990..6379
                     /locus_tag="ISB17_004202"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAP1982074.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            6451..7815
                     /gene="tdcG"
                     /locus_tag="ISB17_004203"
     CDS             6451..7815
                     /gene="tdcG"
                     /locus_tag="ISB17_004203"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP1982075.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
BASE COUNT         1902 a         1879 c         2075 g         2009 t
ORIGIN      
        1 atccacctgt ctgttttgca ggtgtcggtt acggttacct acatatttaa ttcaggctaa
       61 gaggttttat aatgcatatt acatacgatc tcccggttgc tattgatgac atcattgaag
      121 caaaacaacg actggctggg cgaatttata aaacaggtat gcctcgctcc aactatttta
      181 gtgaacgttg caaaggtgaa atattcctca aattcgaaaa catgcagcgt actggttcat
      241 ttaaaattcg tggcgcattt aataaattaa gttcactgac tgatgcggaa aaacgtaaag
      301 gagtagtggc ctgctctgcg ggcaaccatg cgcaaggggt ttcgctctcc tgcgcgatgc
      361 tgggtattga cggtaaagtg gtgatgccaa aaggtgcgcc gaaatccaaa gtcgcggcaa
      421 cgtgcgacta ctccgcagaa gtcgttttgc atggtgataa cttcaacgac actattgcta
      481 aagtgagcga aattgtcgaa atggaaggcc gtatttttat cccaccttac gatgatccga
      541 aagtgattgc tggccaggga acgattggtc tggaaattat ggaagatctc tatgatgtcg
      601 ataacgtgat tgtgccaatt ggcggcggcg gtttaattgc tggtattgcg gtggcaatta
      661 aatctatcaa cccgaccatc cgtgttattg gcgtacagtc tgaaaacgtt cacggcatgg
      721 cggcttcttt ccactccgga gaaataacta cgcaccgaac taccggcacc ctggcggatg
      781 gttgtgatgt ctcccgcccg ggtaatttaa cttacgaaat cgttcgtgaa ttagtcgatg
      841 acatcgtgct ggtcagcgaa gacgaaatca gaaacagtat gattgcctta attcagcgca
      901 ataaagtcgt caccgaaggc gcaggcgctc tggcatgtgc tgcattatta agcggtaaat
      961 tagaccaata tattcaaaac agaaaaaccg tcagtattat ttccggcggc aatatcgatc
     1021 tttctcgcgt ctctcaaatc accggtttcg ttgacgctta attaattcgt tgaggatagg
     1081 atatgagtac ttcagatagc attgtatcca gccagacaaa acaatcgtcc tggcgtaaat
     1141 cagataccac atggacgtta ggcttgtttg gtacggcaat cggcgccggg gtgctgttct
     1201 tccctatccg cgcaggtttt ggcggactga tcccgattct tctgatgttg gtattagcat
     1261 accccatcgc gttttattgc caccgggcac tggcgcgtct gtgtctttct ggctctaacc
     1321 cttccggcaa cattacggaa acggtggaag agcattttgg taaaactggc ggcgtggtta
     1381 tcacgttcct gtacttcttc gcgatttgcc cactgttgtg gatttatggc gttaccatta
     1441 ccaacacctt tatgacgttc tgggaaaacc agctcggctt tgcaccgctg aatcgcggct
     1501 ttgtggcgct gttcctgttg ctgctgatgg ctttcgtcat ctggtttggt aaggatctga
     1561 tggttaaagt gatgagctat ctggtatggc cgtttatcgc cagcctggtg ctgatttctc
     1621 tttcgctgat cccttactgg aactctgcag ttatcgacca ggttgacctc ggttcgctgt
     1681 cgttaactgg tcatgacggt atcctgatca ccgtctggct ggggatttcc atcatggttt
     1741 tctcctttaa cttctcgcca atcgtctctt ccttcgtggt ttctaaacgt gaagagtacg
     1801 agaaagattt tggtcgcgac ttcaccgagc gtaaatgttc acaaatcatc tctcgcgcca
     1861 gtatgctgat ggttgcagtg gtgatgttct ttgcctttag ctgcctgttt acgctgtctc
     1921 cggcgaatat ggctgaagcg aaagcgcaga atattccagt gctttcttat ctggctaacc
     1981 actttgcgtc catgaccggt accaaaacaa cgtttgcgat tacactggaa tatgccgctt
     2041 ccatcatcgc actcgtggct attttcaaat ctttcttcgg tcactatctg gggacgctgg
     2101 aagggctgaa tggtttgatt ctgaagttcg gttataaagg tgacaaaacc aaagtgtcgc
     2161 tgggtaaact gaatactatc agcatgattt tcattatggg ctccacctgg gttgttgcct
     2221 acgccaaccc gaacatcctc gacttgattg aagccatggg cgcaccgatt atcgcatcac
     2281 tgctgtgcct gttgccgatg tatgccatcc gtaaagcgcc gtctctggcg aaataccgtg
     2341 gtcgtctgga taacgtgttt gttaccgtga ttggtctgct gaccatcctg aacatcgtat
     2401 acaaactgtt ttaatccgta actcaggatg agaaaagaga tgaatgaatt tccggttgtt
     2461 ttggttatta actgtggttc gtcttcgatt aagttttccg tactcaatgc cagcgactgt
     2521 gaagtattaa tgtcaggtat tgccgacggt attaactcgg aaaatgcatt cttatccgta
     2581 aatgggggag agccagcacc gctggctcac cacagctacg aaggtgcatt gaaggcaatt
     2641 gcatttgaac tggaaaaacg gaatttaaat gacagtgtgg ctttaattgg ccaccgcatc
     2701 gctcacggcg gcagtatttt taccgagtcc gccattatta ccgatgaagt cattgataat
     2761 atccgtcgcg tttctccact ggcacccctg cataattacg ccaatttaag tggtattgaa
     2821 tcggcgcagc aattattccc gggcgtaact caggtggcgg tatttgatac cagtttccac
     2881 cagacgatgg ctccggaagc ttatttatac ggcctgccgt ggaaatatta tgaagagtta
     2941 ggtgtacgcc gttatggttt ccacggcacg tcgcaccgct atgtttccca gcgcgcacat
     3001 tcgctgctga atctggcgga agatgactcc ggcctggttg tggcgcatct tggcaatggc
     3061 gcatcaatct gcgcggttcg caacggtcag agtgttgaca cttcaatggg aatgacgccg
     3121 ctggaaggct tgatgatggg tacccgcagt ggtgatgtcg acttcggggc gatgtcctgg
     3181 gtcgccagcc aaaccaacca gagcctgggg gacctggaac gcgtagtgaa taaagagtcg
     3241 ggattattag gtatttccgg cctttcttcg gatttacgtg ttctggaaaa agcctggcat
     3301 gaaggtcacg aacgcgcgca actggcaatt aaaacctttg ttcaccgaat tgcccgtcat
     3361 attgccggac acgctgcttc attacatcgc ctggatggaa ttatattcac cggcggaata
     3421 ggagagaatt caagtttaat tcgtcgtctg gtcatggaac atttagctgt attaggcgta
     3481 gaaattgata cagaaatgaa taatcgctct aactcttttg gagagcgaat tgtttccagt
     3541 gaaaatgcgc gtgtcatttg cgccgtaatt ccgactaacg aagaaaaaat gattgctttg
     3601 gatgccattc atttaggcaa agtgaacgcg cccgcagaat ttgcataatt tagttgaagt
     3661 attgtagaga gattattttt catgaaggta gatattgata ccagcgataa gctgtacgcc
     3721 gacgcatggc ttggctttaa aggtacggac tggaaaaacg aaattaatgt ccgcgatttt
     3781 attcaacata actatacacc ttatgaaggc gatgaatctt ttctcgccga agcgacgcct
     3841 gccaccacgg aattgtggga aaaagtcatg gaaggcatcc gtatcgaaaa tgcgactcac
     3901 gcgccattgg atttcgatac caatattgcc accacaatta ccgctcatga cgcgggatat
     3961 attaaccagc cgctggaaaa aatagtgggt ctgcaaacgg atgcgccatt aaagcgtgcg
     4021 ctgcatccgt tcggcggcat taatatgatt agaagttcat tccacgcgta tggtcgtgaa
     4081 atggatagcg aatttgaata catctttacc gatctgcgta aaacccataa tcagggcgta
     4141 tttgatactt actcaccgga tatgctgcgc tgccgtaaat ctggtgtact gaccggttta
     4201 ccagatggtt atggtcgtgg gcgtatcatc ggtgactatc gccgtgtggc actgtacggc
     4261 atcagttatc tggtacgtga acgcgaactg cagtttgccg atctccagtc ccgtctggaa
     4321 aaaggcgaag atctcgaagc caccattcgt ctgcgtgagg aactggcaga acatcgccgt
     4381 gcgctgttac agattcagga aatggcggcg aaatatggct ttgatatctc tcgtccagcg
     4441 cagaatgcac aagaagccgt gcagtggctc tacttcgctt acctggcggc agtgaaatcg
     4501 caaaatggcg gcgcgatgtc gctggggcgc acggcatcgt tcctcgatat ctacattgag
     4561 cgcgacttta aagcaggcgt actcaatgag cagcaggcac aggaactgat cgaccacttc
     4621 atcatgaaga tccgtatggt gcgcttcttg cgtacgccgg aatttgattc gctgttctcc
     4681 ggcgacccga tctgggcgac ggaagtgatc ggcgggatgg ggttggacgg tcgaaccctg
     4741 gtgaccaaaa actccttccg ctatttacac accctgcaca ctatggggcc tgcaccggaa
     4801 cctaacctga ctattctgtg gtcggaagcc ttgccgattg ccttcaaaaa atatgccgcg
     4861 caggtgtcta tcgtcacatc ctctttgcag tatgaaaacg acgatctgat gcgtactgac
     4921 ttcaatagcg acgattacgc gattgcctgt tgcgtcagcc caatggtgat tggtaagcag
     4981 atgcagttct tcggtgcgcg cgctaacctg gcgaaaacgc tactgtacgc aatcaacggc
     5041 ggaatggacg agaagctgaa gattcaggtt ggcccgaaaa cggctccgtt gatggatgac
     5101 gtgctggact acgacaaagt gatggatagc ctcgaccact tcatggactg gctggcggta
     5161 cagtacatca gcgcgctgaa tatcattcac tacatgcacg acaagtacag ctacgaagct
     5221 tcgctgatgg cgctacatga tcgtgatgtc tatcgcacca tggcgtgcgg catcgcgggc
     5281 ctgtcggtgg caacggactc cctgtctgcc atcaaatatg ctcgtgtgaa accaatccgt
     5341 gatgaaaacg gcctggcggt ggatttcgag attgacggcg aatatccgca gtatggcaac
     5401 aacgacgagc gcgtagacag cattgcctgc gatctggttg aacgctttat gaagaaaatt
     5461 aaagctttgc caacctatcg caatgctgtg ccgacccaat cgattctgac tatcacctct
     5521 aacgtggtgt acggtcagaa aaccgggaac acgccggacg gtcgccgcgc cggaacaccg
     5581 ttcgcgccgg gcgctaaccc gatgcacggt cgtgatcgca aaggtgccgt ggcctcattg
     5641 acgtcggtgg cgaaactgcc gttcacctac gccaaagatg ggatctcgta caccttctcc
     5701 atcgtccctg cggcgctggg caaagaagat ccagtacgta aaaccaacct tgtcggcctg
     5761 ctggatgggt atttccacca cgaagcggat gtcgaaggcg gtcaacacct caacgtcaac
     5821 gtaatgaatc gggaaatgct gctggatgcc atcgagcacc cggaaaaata tcctaacctg
     5881 acaatccgtg tctctggcta cgccgtgcgc ttcaacgcac tgacccgtga acagcaacag
     5941 gatgttattt cacgtacctt tacccaggcg ctctgacgcc ggaggatgta tgaaaaagat
     6001 tatcgaaacg caacgtgccc caggcgcaat cggcccttat gttcagggcg ttgatttagg
     6061 cagcatggtc ttcacctccg ggcaaattcc ggtttgccca cagaccggtg agatcccggc
     6121 tgatgtgcaa gatcaggcgc gtttaagcct cgaaaacgtc aaagcgatcg tggttgctgc
     6181 cgggctgagt gtgggcgata tcatcaagat gaccgtgttc atcaccgatc tgaatgattt
     6241 tgccaccatc aatgaagtct ataagcagtt cttcgatgaa catcaggcga cctacccgac
     6301 ccggagctgt gtgcaggtcg cgcgtttgcc gaaagatgtg aagctggaaa ttgaagccat
     6361 cgcagtacgt agtgcgtaaa gcctcgtgag cgggacggtc gcaaggtcgt tccgctccac
     6421 ttcactgaac ggcaatccga gggtgtggat atgattagtg cattcgatat tttcaaaatt
     6481 gggattggtc cctccagttc gcatactgtg gggccaatga atgccggaaa aagttttatt
     6541 gatcggctgg aaagtagcgg cttattaacc gcgacgagcc atattgtggt cgatctgtac
     6601 gggtcgttgt cactgacggg caaaggccat gccacggatg tcgccatcat catgggactg
     6661 gcaggaaaca gtccgcagga tgttgtcatt gatgagatcc ctgcatttat agagttagtg
     6721 acgcgcagcg ggcggttgcc ggtggcttct ggcgcgcata ttgttgattt tcctgtagca
     6781 aagaacatta tcttccatcc cgaaatgttg cctcgccatg agaacggaat gcggatcact
     6841 gcctggaagg gacaggaaga gctattaagc aaaacctatt actctgtcgg cggcgggttt
     6901 attgtcgaag aagaacactt cggcctgtcg cacgatgtcg aaacacccgt accttacgat
     6961 ttccactcag caggtgaact gctgaaaatg tgtgattaca acggcctgtc tatatctggc
     7021 ctgatgatgc acaacgagct ggcgctgcgc agtaaagcgg aaattgacgc agggtttgcc
     7081 cgtatctggc aagtgatgca tgacggtatt gaacgtggaa tgaatactga aggcgtgctg
     7141 cctggtccgc tcaatgtgcc gcgccgtgcc gtagcgctgc gtcgtcaact ggtttccagc
     7201 gataacatct ctaacgatcc gatgaatgtc atcgactgga tcaacatgta cgcgctggcg
     7261 gttagtgaag aaaacgcagc tggcgggcgc gtggtaacgg caccgactaa cggtgcgtgc
     7321 ggcattattc cggcagtgct ggcttattac gataagttcc gtcgtccggt aaacgagcgg
     7381 tcaattgccc gctattttct ggcagcgggg gcaattggcg cgctgtataa aatgaacgcc
     7441 tccatctctg gcgcggaagt cggctgtcag ggggagattg gcgtggcctg ttcaatggca
     7501 gcggcagggt taactgaact actgggtggc agtccggcgc aggtatgcaa tgcggcggaa
     7561 atcgcgatgg agcataacct tgggctgacc tgcgatccgg ttgccggaca ggtacaaatc
     7621 ccgtgcattg aacgtaatgc cattaatgcc gtgaaagcag taaacgccgc gcggatggcg
     7681 atgcgccgca cctcggcacc gcgtgtttca ctcgataaag tgatcgagac gatgtatgaa
     7741 accggcaaag atatgaacga taaataccgc gaaacatcac gcggaggact ggctattaaa
     7801 gtggtctgcg gctgacattg ctgtcagcta tcatccttgg gtgcacaaaa tgtgcaccct
     7861 ttttt
//
LOCUS       DACADA010000190         7842 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.192, whole genome shotgun sequence.
ACCESSION   DACADA010000190 DACADA010000000
VERSION     DACADA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.192"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            88..177
                     /gene="kdpF"
                     /locus_tag="ISB17_004204"
     CDS             88..177
                     /gene="kdpF"
                     /locus_tag="ISB17_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAP1982076.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            177..1850
                     /gene="kdpA"
                     /locus_tag="ISB17_004205"
     CDS             177..1850
                     /gene="kdpA"
                     /locus_tag="ISB17_004205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAP1982077.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1873..3921
                     /gene="kdpB"
                     /locus_tag="ISB17_004206"
     CDS             1873..3921
                     /gene="kdpB"
                     /locus_tag="ISB17_004206"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAP1982078.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3930..4502
                     /gene="kdpC"
                     /locus_tag="ISB17_004207"
     CDS             3930..4502
                     /gene="kdpC"
                     /locus_tag="ISB17_004207"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAP1982079.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            4495..7179
                     /gene="kdbD"
                     /locus_tag="ISB17_004208"
     CDS             4495..7179
                     /gene="kdbD"
                     /locus_tag="ISB17_004208"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAP1982080.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7176..>7842
                     /gene="kdpE"
                     /locus_tag="ISB17_004209"
     CDS             7176..>7842
                     /gene="kdpE"
                     /locus_tag="ISB17_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAP1982081.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYR"
BASE COUNT         1608 a         2070 c         2335 g         1829 t
ORIGIN      
        1 ttacaccacg cccgcagatt tttgcgaaat ctttgcagcc agaactctac ccttccggta
       61 tcacttttag gccactggag gtgcactgtg agtgcaggcg tgataaccgg cgtgttgctg
      121 gtgtttttat tactgggtta tctggtttat gccctgatca atgcggaggc attctgatgg
      181 ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg ctggcgcgtc
      241 ccttaggtag cgggctggcg cggctgatta atgatattcc tcttcctggt acgacgggcg
      301 ttgaacgcgt actctttagc gcgcttggcg tctctgaccg cgagatgaac tggaagcagt
      361 atctctcagc cattcttggc ctgaatatac tggggctggc ggtgctgttt tttatgttgc
      421 tcggtcagca ctatctgccg cttaatccac agcagttgcc agggctgtcg tgggatctgg
      481 cgctcaatac cgccgtcagc tttgtcacca ataccaactg gcaatcttat agcggtgaaa
      541 ccacgttgag ctatttcagc cagatggcgg gcttaacggt acaaaacttt ctttctgccg
      601 ccagcgggat tgcggtgatt tttgccctca ttcgtgcatt tacccgccag agcatgagca
      661 cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgcta gtccctgtgg
      721 cgttgttgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt ctgccctatc
      781 aggcggtgaa taccgttgaa ggagcacaac agctgttacc catggggcca gtagcttctc
      841 aggaagcgat caaaatgctc ggaactaacg gcggtggctt ttttaatgcc aactcgtcgc
      901 atccgtttga aaacccaacc gcactgacca acttcgtgca aatgctggcg attttcttga
      961 tcccaacagc gctgtgcttt gcctttggtg aagtggcggg cgatcgtcgc caggggcgca
     1021 tgttgctgtg ggcgatgtcg gtgatttttg tcatctgcgt aggcgtagtg atgtgggcag
     1081 aagttcaggg taatcctcat ctgctggcgc tcggcgcgga cagcagcatc aatatggaag
     1141 gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg actacggcgg
     1201 cttcctgcgg cgcggtgatt gcgatgcatg attcgtttac cgctcttggt ggcatggtgc
     1261 cgatgtggtt gatgcaaatt ggtgaagtgg tattcggcgg cgtcggttcc ggcctttacg
     1321 gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt ggtcgtacac
     1381 cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgactgca ctggcgattc
     1441 tggtcacccc gacgctggtg ctgatgggcg cggcgctggc aatgatgacc gacgccggac
     1501 gtagcgccat gctcaaccct ggcccgcatg gttttagcga agtgctgtac gccgtgtcat
     1561 ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct ccattctgga
     1621 actgtttact ggcgttgtgc atgtttgtcg ggcgcttcgg ggtgattatc ccggtgatgg
     1681 caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc acattgccaa
     1741 cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt ggcgcactga
     1801 cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga tgatattgag
     1861 tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact tgtcgttcag
     1921 gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa tccggtgatg
     1981 tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat ggcaagcggt
     2041 gtgatgcctg gtaatgcgct gtttagcgcg gcaattagcg gctggctgtg ggtcaccgta
     2101 ctgttcgcta atttcgccga ggcgttggca gaaggccgca gtaaagcgca ggccaacagt
     2161 ctgaaagggg tgaaaaaaac cgcttttgct cgcaagctgc gcgaaccgaa atatggcgct
     2221 gtggcagaca aagttcctgc cgatcaactg cgtaaaggcg atatcgtact ggtagaagct
     2281 ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt cgatgaaagc
     2341 gccatcaccg gggaatcggc tccggtgatc cgtgaatccg gcggtgattt tgcctccgtc
     2401 actggcggca cgcgtattct ttctgactgg ctggtgattg agtgcagcgt taaccccggc
     2461 gagacatttc tggatcggat gatcgcgatg gtggaaggtg cgcagcgacg taaaacgccg
     2521 aacgagattg ccctgaccat tctcctgatt gccctgacta tcgtcttttt actggcaacc
     2581 gccacgctgt ggccgttttc cgcgtggggc ggtaatgcag tcagcgtaac ggtactggtg
     2641 gcactgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc gattggcgtc
     2701 gccgggatga gtcggatgct gggcgcgaat gttattgcca ccagcgggcg tgcagttgaa
     2761 gcggcgggcg acgtggacgt cctgctgctg gataaaaccg gcaccatcac actcggtaac
     2821 cgtcaggcgt cggagtttat ccctgcgcag ggcgtggatg aaaaaacgct ggctgacgcc
     2881 gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc gcagtattgt gatcctcgcc
     2941 aagcagcgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac ctttgtaccg
     3001 tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat ccgtaaaggt
     3061 tctgtcgatg ccattcgccg ccatgttgag gctaacggcg gtcacttccc tgccgatgtt
     3121 gatcaaaaag tcgatcaagt tgcgcgtcag ggagccacgc cgctggtggt ggtggaaggt
     3181 tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat taaagagcgc
     3241 ttcgcccagc tgcgtaaaat gggcattaaa acggtgatga ttaccggcga taaccgtctg
     3301 actgccgccg cgattgccgc ggaagcgggc gtcgatgatt ttctcgccga agcaacaccg
     3361 gaggccaagc tggcgttgat tcgtcagtat caggcagaag gccgtttagt ggcgatgacc
     3421 ggcgatggca ccaacgatgc tccggcgctg gcgcaggcgg atgtcgcggt ggcgatgaac
     3481 tccggcaccc aggcggcgaa agaagcaggc aatatggtgg atctcgactc caacccgacc
     3541 aagttgatcg aggtggtgca cattggcaaa cagatgctga tgacccgtgg ctcgttgacc
     3601 accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccagc ggcgttcgcg
     3661 gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc cgactccgcc
     3721 attctcagtg cggtcatttt caacgccttg attatcgtct ttttgattcc cctggcgtta
     3781 aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa cttatggatt
     3841 tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga tttactgcta
     3901 accgtttgcg gtctggtgtg aggtttacca tgagtggatt acgtccggca ttatcgacat
     3961 ttctctttct gttattgatt actggcggcg tttacccgct gctgaccacc gcactggggc
     4021 aatggtggtt tccctggcag gccaacggtt cattgattcg tgaaggtgat acggtgcgcg
     4081 gttcggcatt aatcgggcaa aattttaccg gcaacggcta ttttcatggt cgcccgtcgg
     4141 caacagcaga aatgccctat aatccacagg cttctggcgg gagcaatctg gcagtcagta
     4201 acccggtgct ggataaacag atagccgcac gcgttgccgc attacgggcc gctaacccgg
     4261 acgccagcac gagcgttccg gttgaactgg tgacagcgtc agcaagcggg ctggacaata
     4321 atattacccc gcaagcggcg gcctggcaga tcccacgcgt ggcgaaagcg cgtaatctca
     4381 gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca acaaccgctg gtgaactata
     4441 tcggccagcc ggtcgtcaac gttgtagaac tcaatctggc gctggataag cttgatgaat
     4501 aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc gccgcatcgg
     4561 gggaagctga aagttttctt cggtgcctgc gcaggcgtcg gaaagacctg ggcgatgctg
     4621 gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt ggtagaaacc
     4681 cacgggcgga aagataccgc cgcaatgctg gaaggtctgg ctgttctgcc gctgaaacgc
     4741 caggcgtacc gtgggcggca tatcagcgag tttgatctcg atgccgccct cgcccgccgt
     4801 ccggcactga tcttaatgga cgaactggcg cacagtaatg cgccaggttc tcgtcatcct
     4861 aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt cactaccgtc
     4921 aacgttcagc atctggaaag cctgaacgat gtggtcagtg gcgtaaccgg aattcaggtt
     4981 cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtagtact ggtggacttg
     5041 cccccggacg atctgcgcca acgactgaaa gaaggcaaag tctatattgc cgggcaggca
     5101 gaacgcgcca ttgagcattt tttccgcaaa ggcaatctga tcgccctgcg cgagctggcg
     5161 ctacgccgta ctgccgatcg cgtagatgag caaatgcgcg cgtggcgggg gcatcctggc
     5221 gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcgggca taacaccggc
     5281 agtgagaaac tggtccgcgc ggcggcgcgg ctggcgtcgc gcctgggcag tgtctggcac
     5341 gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg tcgggcaatt
     5401 ctcagcgcct tacgtctggc gcaggagttg ggcgcggaga cggcaacact ttctgatcca
     5461 gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc
     5521 ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg gctggcgcgc
     5581 atcgcccccg atctcgatca ggtactggtc gcgcttgatg aaccacccgc ccgcacgatt
     5641 aataacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc
     5701 gtggttgccg ccgcgttatg cgccgttatc accttaattg ccatgcagtg gctgatggcg
     5761 tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggttgtggt ggcactattt
     5821 tacggacgct ggccttcggt ggttgcgacc gtcattaatg tggtgagttt cgatctcttt
     5881 tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgcg
     5941 gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg
     6001 cgggtagccc gttaccgcga acaacgcaca cggcacttat atgaaatgtc caaagctctg
     6061 gctgtgggcc gtagttcgca ggatatcgcc gccaccagcg aacagtttat tgcctccacg
     6121 tttcatgccc gtagccaggt gttgctgcct gatgacaacg gtaaattgca gcctttaaca
     6181 catccgcaag gaatgacgcc gtgggacgat gccatcgcgc agtggagttt tgataaaggc
     6241 ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat tttgccgcta
     6301 aaaagcggcg agaaaaccta cggactggtg gtggtggaac cggggaatct gcgccagttg
     6361 atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc caatgccctt
     6421 gagcgactga cactgaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag
     6481 atccgcaacg ccctgctggc ggcgctttcg catgatttac gcacgccgct tacggtgctg
     6541 ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc
     6601 caggccagcg agattcgtca gcatgtgctg aacaccaccc gactggtgaa taatctactg
     6661 gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa
     6721 gaagtggtcg gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc catcaatctt
     6781 tctctgccag agccgctgac cttaatccac gttgacgggc cactctttga acgggtgctg
     6841 attaacctgc tggagaacgc ggtgaaatat gcgggggcgc aggccgaaat tggtatcaat
     6901 gcccacatcg agggcgaaaa tctacaactg gatgtctggg ataacggccc cggtcttcca
     6961 ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcggta
     7021 ccgggtgtag ggcttgggtt ggcaatttgt cgggcgatag tggatgtaca cggcggcacc
     7081 attaccgcat tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa
     7141 acagcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg attgttgaag
     7201 atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgatggg atgcgcgtct
     7261 ttgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga
     7321 ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc
     7381 agtggagcgc ggtgccggtg attgtgcttt ccgcacgtag cgaagagagc gacaaaatcg
     7441 ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc
     7501 aggcccgtct gcgcgtcgct ttacgtcgcc actccgccac cgccgcgccc gatccgctgg
     7561 tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt tatccatcgg ggcgaggaag
     7621 aggtgcatct caccccgatt gagttccgcc tgctggcagt gttgctcaac aacgccggaa
     7681 aagtgctcac ccagcgccag ttactcaatc aggtgtgggg accaaacgcg gtcgaacaca
     7741 gccactattt gcgtatttat atggggcatc tgcgacaaaa actggagcaa gatcccgccc
     7801 gcccccgcca ttttattact gaaaccggta ttgggtatcg gt
//
LOCUS       DACADA010000191         7699 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.193, whole genome shotgun sequence.
ACCESSION   DACADA010000191 DACADA010000000
VERSION     DACADA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.193"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            89..331
                     /locus_tag="ISB17_004210"
     CDS             89..331
                     /locus_tag="ISB17_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982082.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYLRKLREKW"
     gene            complement(513..1442)
                     /gene="fdhE"
                     /locus_tag="ISB17_004211"
     CDS             complement(513..1442)
                     /gene="fdhE"
                     /locus_tag="ISB17_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAP1982083.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1439..2074)
                     /gene="fdoI"
                     /locus_tag="ISB17_004212"
     CDS             complement(1439..2074)
                     /gene="fdoI"
                     /locus_tag="ISB17_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1982084.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2071..2973)
                     /gene="fdxH"
                     /locus_tag="ISB17_004213"
     CDS             complement(2071..2973)
                     /gene="fdxH"
                     /locus_tag="ISB17_004213"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP1982085.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2986..6036)
                     /gene="fdnG"
                     /locus_tag="ISB17_004214"
     CDS             complement(2986..6036)
                     /gene="fdnG"
                     /locus_tag="ISB17_004214"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5449..5451),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP1982086.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6230..7063
                     /gene="fdhD"
                     /locus_tag="ISB17_004215"
     CDS             6230..7063
                     /gene="fdhD"
                     /locus_tag="ISB17_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAP1982087.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7151..7639
                     /locus_tag="ISB17_004216"
                     /pseudo
     CDS             7151..7639
                     /locus_tag="ISB17_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1765 a         2007 c         2054 g         1873 t
ORIGIN      
        1 aaaagtgatg cgtaaatgcg ctttctttaa agattcgaca agggtacttt tctctccata
       61 gattcaaaat gatattggcg agatatttat gaaccctctg gcaggtatgg atatgagcag
      121 aattttactc gatttatcga atgaggtgat taagcaactt gatgatcttg aggtgcagcg
      181 taatcttccg cgtgcagacc tattacggga agcggtagat caatacctgg taaatcaatc
      241 gcaaacagca agaaccagtg ttcctggcat ctggcaaggg tgtgaggaag atggtgtcga
      301 atacctgcgt aagctgcgcg agaaatggta atggcaaaaa gatttggcta tttgatatcg
      361 atatccttat cgattttctc agcgtgaaaa ttgaggccaa acaggcactg gcggcatagc
      421 ttgaggaggt aacgcggaat acgagagata gtgtcgtcac accctatgaa ctgtaatccg
      481 ggcgacctgc gccacccgga aaacgtcaga gattactccc cttcacccgg aaacaggaac
      541 gggttgatgg aactacgggc atagccttct tgctccattc gcgcgtccag taccagagag
      601 gcgaggtcat ctgccacggc ttcaactttc ggttcttttt cctgataaag aatcttcagg
      661 taagtgccgc agtcatcgca gctttcggct ttaatcgcgg cctgttcgtc atccagcgac
      721 cagtaatgca gtttgccgct ctgttcacag ttgctgcatt ttacgcgcac tacgtgccat
      781 tcagtttcac acaggttgca gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc
      841 atgctggaca ccggcataga gccacatacc gggcaatatt gacgttgttc gccgtattca
      901 gcgcgggctt tgccggggat cagattagcc atctgcgccc agtagagcga cagcgcagcc
      961 cagataaacg gcgctttatc gctgctgacg gacgagaaat cagaggcaaa cagtgcgctg
     1021 gccatatctt ccagctcctg agtcgatgcc ttctccagat tctcaatcac tgccagcgcc
     1081 gggccgctca tttcaggttt cagctcagca atcagcgcca tcagcagctt ttgccagtgc
     1141 ttatcacgcg gcagaacgtg aatatccagc gggggcttgc cttgtgcgct ggcttcttta
     1201 atgcgcgtgg tcagatccat ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg
     1261 gcgataagcg cagcaaagcg caggtaatca cccagcggat tatgttctgc cagctcgcgc
     1321 agacgctcgg cgcggcggtt gtataaattc ttgagccgag ggaacaataa cggcggaatc
     1381 atatccgccg tacgtttctc gctcgaaccc agctcatctt gcgggattat gcgaatactc
     1441 attcagcttt cttttccgtt gtcttgcgga cctcacggta ccagcgcggg tgatgtttct
     1501 ttgcccatgc gctggtcacc catccttcca ccatggcggt aatcgtgcct ttcacccaaa
     1561 gggcggcgta gatatgcacc atgataacca caattaacgc cactgcggca aatgaatgca
     1621 gcattaacgc gaatcggatc accgggattg agaaagcagg cgcaaaataa ggacgccaga
     1681 taatcacgcc gctcaccagc aacaggacca ggaaaataat cgccgcccag aaaacgcatt
     1741 tctgaccgaa gttataacgc ccggtgtcac ctacttcctc gttgacgacg atcttacgaa
     1801 tattcttcgc ccaaaagata tcatcccgat tgattaggtt gtgatgccag taacggaaaa
     1861 acatgatgat gaacgaggca aacataacca cgccgacaaa cggatgcaga attcgcgcca
     1921 gctgcggtgt gcccatgatt tgcatcaacc agttgaagga cgggaacaaa aagcccagcc
     1981 cgctcaccgc cgccaggatg aagcagaagg cggtgatcca gtggttgata cgttccggcg
     2041 cggtgtagcg cacgatggtg tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg
     2101 cagattattc tcttcctcat ccgcacggtt cggaccgaca cccacgtagt ggaagatact
     2161 ggctgcgaag gtagccgcaa agccaacagc tgcgagtggt ttccagatgc ctttccagaa
     2221 tttcacggtt tcgctgattt ccgggttctc cggcaagcca tgatacagat ttggcttgtc
     2281 agcatggtgc agtacgtaca taacgtgtgt accaccgacg cctgccggat cgtacagacc
     2341 cgcattgtcg taaccgcggg ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat
     2401 cgactctttc gtaccgaagt gaatcgcgcc cgttgggcag gtcttcacgc aggccggttc
     2461 ttgcccaacc accacgcggt caacgcacaa cgtacatttg tagacgcggt tgtcttccgg
     2521 gttgaggcgt ggcacgttga acgggcaccc tgcgatgcaa taaccgcagc cgatgcactg
     2581 ctcggactgg aagtcgacga taccattggc atactgaata attgcccctt ccgccgggca
     2641 tgctttcagg cagcctggat cggaacagtg catacagccg tctttgcgga tcagccattc
     2701 cagtttgtcg ttctgctcca cttccgagaa tcgcatcacc gtccacgatt tggcacttaa
     2761 atcattgggg ttgtcataca ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca
     2821 ctctgaacac gccacctgac aggctttaca gccgatacag gtggtaacgt cgatgagttt
     2881 cgccacttct tcctggaagt cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga
     2941 acgacgaatg atatcttgcg attgataagc catatgtcgt ctccgttaca ccttttccac
     3001 attcacaagg aaggacttaa actccggcgt ctgcgtgttc gcatcaccga cgaatggcgt
     3061 caacgtattg gcaataaagc cttttttcgc aacaccttca tagccccagt gaatcggaat
     3121 accgatggta tcgatatctt tgccgtttgc tttcagcgtg cgaatacgtt tggtcactac
     3181 cgctttggct ttgatatagc cacggttgga ggagactttc acagtatcgc cctgggcaat
     3241 gccaagttta ttcgccagcg actccccgat ttccacaaac tgctctggtt gcaaaatcgc
     3301 gttcaacagc gcgtgtttgg tccagtagtg gaagtgctcg gtcagacgat aggtggttcc
     3361 gacatacggg aacttatcgg ctttccccaa tgcttcggca tcgtctttaa agatacgcgc
     3421 cgccggattc gagataacgt ttggatgcag cgggttagtt cccagcggcg tttcaaacgg
     3481 ctcgtagtgt tccgggaacg gaccttccgc catcttatcg agggcaaaca gacgccccat
     3541 gccttcctgc tgcatgataa acggcccgac gccactgccc ggaggcgctg cgctgtagtc
     3601 cggaatatcc cagccggtcc acttagtgcc gtcccatttc agcaactgac gcttcggatc
     3661 ccacgggtta ccctgcggat ctgcggaggc gcggttatac agaatgcggc ggttaagcgg
     3721 ccatgcccat gcccagccca gcgtgttacc gaggccagac ggatcggcgt tatcacgacg
     3781 cgccatctgg ttgccttctg gcgtccagct accggcgaaa atccagcagc cacaggaggt
     3841 tgtaccgtca tcgcgcagtt gagcgaacga gctaagttgt tggcctttct tgacaataac
     3901 cgcaccggtt gccggatcgg taatatcggc cagcgcctta ccgttgctct ccatcgccac
     3961 ttcttccgac ttcggctcat gcgggatggc gtagttccag gtcatgttca gcacctggtc
     4021 cgggttcgcg ccaccctgtt cggcatacat cttgcgcaag cgcaggaaga taccggagag
     4081 gatctcgcca tcagtcagcg caatccccgg ggcgtccgca cctttccagt gccactgcaa
     4141 ccagcgacca gagttaacga ttgaaccgtt ctcttccgcg aagcaggtcg atggcagacg
     4201 gaacacttcg gtctggatct tcgacgagtc aacttcgttc agttcaccgt ggttctgcca
     4261 gaagttagag gtttcagtgt tcagcgggtc gatagtcacg aggaatttca gcttcgacag
     4321 acagccgatc actttgtttt tgttcgggaa tgaggcaaca gggttaaagc cctggcagat
     4381 atagccattg accttgccct ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt
     4441 atcccacttc ggcaaccagt caaagcccca gctattttcc gccgtcgctt tatcaccaaa
     4501 gaaggccttc atcatagaga cgaagaattt cgggtagttg ccccagtagt taacctggcc
     4561 ttccagcagt ggtttcggcg tgttggcggt aaggtaagtt tgcagatcgg tctgcttctc
     4621 gcttggcagc gtcatgtaac ctggcagact ctgcgacagc agccccaggt ccgtcagccc
     4681 ctgaatattg gagtgaccgc gcagggcgtt aacgccgccg ccagccatcc ccatattgcc
     4741 gagcagcaac tggatcatcg ccatcgtacg gatgttctgc gcaccgatgg agtgttgcgt
     4801 ccagccgagg gcgtaaagga acgaggcagt tttatcgtga gcactggtct ctgcgatgta
     4861 ttcgcagact ttcaggaatg cgtcttttgg cgtaccgcag atgttttcaa ccacatctgg
     4921 cgtgtagcgg gaaacgtgct gtttcagcaa gttccacacg caacgtggat gttgcagggt
     4981 agtatcgcgt ttggcgaagc cgttttcgtc cagttcgtaa gtccaggtgg acttatcgta
     5041 cttgcgtttt tccgcgtcgt agccggtgaa caggccatct tcaaagccgt aatcctcacg
     5101 cacgatcagg ctagcgttgg tataggcttc ggtgtattcg cggttgaatt tttcattgtt
     5161 cagcaggtac agcaatacgc ctgacaggaa agcaatgtca gtaccggaac ggataggggc
     5221 atagtagtca gccaccgccg ctgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc
     5281 gccgttgtga attttggctt ccatcgccca gcggaacccg accgggtgag cttcagcggc
     5341 gttaccgccc atcaccacga cgaggttggc attcttgatg tcgacccagt ggttggtcat
     5401 cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc
     5461 ctggttgtcg accgcgagca tacccagcgc gcgggagaat ttttgcgtta aatagccggt
     5521 ttcgttgctc gacgcggaag cacacagcat cccggtggag agccagcggt taacagtcac
     5581 gccttcggcg ttttgcgcaa tgtagtttgc atcgcggtct tctttcatca gtttggcgat
     5641 gcgatcaaac gcctcttccc aactgatttg ctgccattta tcagaacctg gcgcacggta
     5701 ttccggaaac ttcagacggc tttcggagtg gatgaaatcc accaggccag cgcctttcgg
     5761 acaaagtgct ccgcggttga ccgggtgatc cgggtcacct tcgatatgga agatagatgc
     5821 tttggcgttt tttgctccgt caccgaggct gtacatcaac agcccacagc ctacggaaca
     5881 ataggtgcag gtattacggg tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc
     5941 gagtgctacg ctgggtgcaa aacccagtgc cgctgccgtg gtgcctgcca taccgccagc
     6001 gcagatctta aagaactgcc ttctgctgac ctgcatggat tgctccttgt ttcgacattg
     6061 tcacgtccca tttacattcg cttgctgcgt gtgcagggag tgggagttat ttttctttgc
     6121 ggaaggggcc gcaaaggtcc agaattggct caatttccct ccatccagga agggtttgta
     6181 acagaatacc ataatgttgg tgtgtgtgtt cttatctggt taatagaaag tgaaaaaaac
     6241 acagcaaaaa gaaatcgaga atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg
     6301 gcggcgtgat gatttacaac acccacggtt agatgaggtg gcggaagaag ttcccgttgc
     6361 gctggtctac aacggcattt cgcacgtggt gatgatggcg tcgcccaaag accttgagta
     6421 ctttgcgctc ggtttttcgc tttccgaagg aattatcgaa agcccgcgcg atatcttcgg
     6481 catggatgtc gttccttcct gtaatggcct ggaagtacaa attgagcttt ccagccgccg
     6541 ctttatgggg ttgaaggaac gtcgccgggc gctggcggga cgtacgggct gtggcgtatg
     6601 cggtgtagaa cagcttaacg atatcggtaa accggtgcag ccgctgccgt tcacccagac
     6661 gtttgatctc aacaaactgg atgatgcatt acgtcatctc aatgatttcc agccagtggg
     6721 gcaactgact ggttgtactc acgccgctgc atggatgttg ccatctggcg aactggtcgg
     6781 cgggcatgaa gacgtgggtc gccatgtggc gctggacaaa ctgttaggcc gccgatcaca
     6841 agaaggggaa agctggcagc aaggtgcggt gctggtttcc agccgtgcca gttatgaaat
     6901 ggtgcaaaag tcggcgatgt gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac
     6961 acttgctgta gaagtggctg agcgctgtaa tctgacgctg gtgggctttt gtaaaccggg
     7021 tcgggcgacg gtttataccc atccgcagcg tttgagcaat taattaaaat aagtcctgcg
     7081 atatatatta aattacagga cttattcatt tcgtgaattt tgttattttt ttataaacaa
     7141 aacgcgtgta gtggcgttaa tgcctgcgct gcaacctatt gatggcgtgg cggtgagtta
     7201 tattgatgct gccgtagcgt tgggtaatac cattaacgaa atggataaat attacaccca
     7261 ggaaaattat aaagacgatg cgtttgcgaa agggaaaaca ttccactact actctctggc
     7321 agtcatgatt tcagcgaaac aaattaataa cctgatatcg caagataagt ttgatgcaga
     7381 agcggcaatg aagaaagtgt ctgaactgga aacgctggtg gcgcaagcca aagaagcgga
     7441 taaaagcggc atgaatttct cgtttattaa ttcggcaggc cagtatcaac tcgaggctaa
     7501 aaaatacgtt cgccgcatca gagataaagt cccgtactct gactgggata aagagcaact
     7561 tcaggacgca aactcaagct ggatggccga agactcattt ccacgagcgt tatgcgatta
     7621 caacgaaatg gtcgatgaaa tatttcagtt aattgtaatc gcggggcggg tttgtgatga
     7681 gcacggatat gtcacgaag
//
LOCUS       DACADA010000192         7643 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.194, whole genome shotgun sequence.
ACCESSION   DACADA010000192 DACADA010000000
VERSION     DACADA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.194"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            12..818
                     /locus_tag="ISB17_004217"
     CDS             12..818
                     /locus_tag="ISB17_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1982088.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            828..1235
                     /locus_tag="ISB17_004218"
     CDS             828..1235
                     /locus_tag="ISB17_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAP1982089.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            1235..1729
                     /locus_tag="ISB17_004219"
     CDS             1235..1729
                     /locus_tag="ISB17_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAP1982090.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1795..2592
                     /locus_tag="ISB17_004220"
     CDS             1795..2592
                     /locus_tag="ISB17_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP1982091.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            2603..3427
                     /locus_tag="ISB17_004221"
     CDS             2603..3427
                     /locus_tag="ISB17_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP1982092.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNVVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3479..4708
                     /locus_tag="ISB17_004222"
     CDS             3479..4708
                     /locus_tag="ISB17_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1982093.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5090..5779
                     /gene="pepE"
                     /locus_tag="ISB17_004223"
     CDS             5090..5779
                     /gene="pepE"
                     /locus_tag="ISB17_004223"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAP1982094.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5869..7500)
                     /locus_tag="ISB17_004224"
     CDS             complement(5869..7500)
                     /locus_tag="ISB17_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP1982095.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         1820 a         1955 c         2055 g         1813 t
ORIGIN      
        1 aggagtctgt tatgcaaacg tggttaaatt tgcaggataa aatcattatt gtcaccggtg
       61 gcgcatccgg cattggtctg gcgattgtgg atgaattatt agcacaaggc gcgaatgtac
      121 agatggtcga tattcacggt ggcgatggtc agtatgaaag ccataaaggt tatcagttct
      181 ggccgaccga tatttccagc gccaaagagg taaatcatac ggtagcagaa attatccagc
      241 gttttggtcg catcgacggt ctggttaaca acgccggggt caatttcccg cgtctgctgg
      301 tcgatgagaa agcgcctgcc gggcagtatg aactcaacga agctgcattc gaaaaaatgg
      361 tcaatatcaa ccagaaaggc gtttttctga tgtcgcaggc ggtggcgcga cagatggtca
      421 aacaacatga tggcgtgatt gtgaatgttt cctcagaaag tgggctggaa ggctcagaag
      481 gccaaagctg ttacgccgcg accaaagccg cgctcaatag cttcacgcgc tcctggtcga
      541 aagagctggg taagcacggt atccgtgtgg tcggtatcgc gccggggatt ctggaaaaaa
      601 cagggctgcg cacgccggaa tatgaagagg cgctggcgtg gacgcgcaat atcaccgtcg
      661 agcagctgcg tgaaggctat accaaaaacg ccattcctat tgggcgcgcc ggaagattag
      721 cagaagtggc tgattttgtt tgttatctgc tgtctgaacg cgccagctat atcaccggag
      781 taaccactaa cattgcgggc ggcaaaacgc gcggctaagg aggctttatg gtcaatgcca
      841 ttttttgtgc gcatggcaaa ttagcttgcg ccatgctgga atcggttcag atggtttacg
      901 gcgatgccag ggtcgaggca gttgaatttg tgcccggcga gaacgccgga gacatcgtag
      961 caaagctgga aaagttagta agcattcaca atcaagatga gtggctgatt gccgtggatt
     1021 tacagtgcgg cagcccgtgg aatgcggcag cgatgcttgc gatgcgcaat cctcgtctgc
     1081 gggtaatcag cggcctttct ctgccgctag cgctcgaact ggttgataac caggacagca
     1141 tgaatgttga tgaactgtgt gaacacctga cgcaaatcgc aaagcaaacc tgcgtcgtct
     1201 ggagacaggt ggcaacagcg gaggaggatt tctgatgaac attacgctcg cccgcattga
     1261 tgaccgcctg atccacggcc aggtcactac cgtctggtct aaagtggcta acgcccagcg
     1321 cattattatc tgcaatgacg aagtttataa cgatgaagtt cgccggacat tattgcgtca
     1381 ggctgcgcca ccgggaatga aggttaacgt ggtcaatatt gaaaaagccg tcgcggtgta
     1441 tcacaacccg caatatcagg acgaaaccgt tttttatctt ttcactcgcc cacaggatgc
     1501 cttagcaatg gttcgccagg gggtaaaaat aggtaccctg aatattggcg gcatggcctg
     1561 gcgtcctggt aaaaaacagt taaccaaagc agtttcatta gatgacgatg atattaacgc
     1621 atttcatgag ttaaataatc ttggcgtcat actggattta cgcgtcgtag cgtcagatcc
     1681 ctcaatcaat attatcgaca aaataaatga acagttaatc gcgaattaaa taatagcgcc
     1741 tgtgtacata tgctttaaca ggcagggatg acgacactta aaaggtgaca aatcatggaa
     1801 ataagtactc tacaaatcat tgctatattt cttttttcct gtattgccgg aatgggcagc
     1861 gtgctggatg aatttcagac tcatcgtccg ttaattgcct gtacggtgat tggtttaatt
     1921 ctcggtgatt taaaaaccgg aattatgctc ggtggtacgc tggaattgat agctctcggc
     1981 tggatgaacg tcggcgcggc gcaatctccg gattctgcgc tcgccagcat aatctccgcc
     2041 attctggtta tcgttggtca gcaaagcatc gccaccggaa tcgccatcgc gttgcctgtg
     2101 gctgcggcag gccaggtgct gacggtgttt gcccgtacca tcaccgtagt gttccagcac
     2161 gcggcggata aagcagcgga agaagcacgg tttcgcactc tcgatattct gcatgtctcc
     2221 gcgcttggcg tgcaggcact gcgcgttgct attccggcac tgattgtctc gctgttcgtc
     2281 agcgctgata tggtgagcaa tatgctgagc gccattcccg aatttgtgac ccgtggactg
     2341 cagattgctg gcggttttat cgtggtggtc ggttacgcca tggtgcttcg catgatgggc
     2401 gtgaaatatt tgatgccttt ctttttcctc ggtttcctcg caggtggcta cctcgatctc
     2461 agtctgctgg cgttcggtgg cgtcggcgtg atcatggccc tgctctacat ccagttaaat
     2521 ccacagtggc gtaaagctga accacatccc cagaccacca ctatcaccgc ccttgaccaa
     2581 cttgatgatt aacggagcca tcatggaaca gagaaaaatt acacgcagcg atctggtgag
     2641 catgtttctg cgctccaacc tgcaacaggc atcctttaac tttgaacgta ttcacgggct
     2701 gggtttttgc tacgacatga tccccgccat caaacgactt tacccattaa aagaggatca
     2761 ggttgcggcg ctcaggcgac acctggtgtt cttcaatacc acgccagccg tatgtggccc
     2821 ggtcatcggc gtcactgccg ccatggaaga ggcgcgagcc aacggcgcgg aaattgatga
     2881 cggtaccatt aacggcatca aagtcggtct gatgggacca ttggcaggag ttggcgatcc
     2941 actggtctgg ggaacgctgc gcccgattac cgccgcgctc ggcgcatctc tggcactttc
     3001 gggcaacatt ctcggccccc tgctgttctt ctttattttc aacgtggtgc gcctggcgat
     3061 gaagtggtat ggcctacagc tcggctttcg caaaggggtg aatatcgtca gcgatatggg
     3121 cggtaatttg ctgcaaaaac tcaccgaagg cgcgtcgatt ctcgggctgt ttgtgatggg
     3181 cgtgctggtg accaaatgga cgtcaatcaa cgtaccgttg gtggtttcac aaacgcctgc
     3241 cgccgatggt gccaccgtca ccataaccgt gcagaacatc ctcgaccaac tttgccctgg
     3301 tttgctggcg ctcggtctga cgctgctgat ggttcgtctg ctcaacaaaa aaattaaccc
     3361 ggtatggctg attttcgccc tgtttggttt agggattatc ggtaatgcgc tgggcttcct
     3421 gtcctgattc ttcgccccgg cgcaaccgtc ggggccatcg ttcaacacga ggtggtttat
     3481 gaaaacaaca gctctgcgtc tttatggtaa acgtgattta cgcctggaaa cctttgacct
     3541 tcctgaaatg caggaggatg aaatcctcgc aacggtggtc actgacagcc tgtgcctctc
     3601 ttcctggaaa gaggccaatc tgggtgaaaa tcataaaaaa gtacccgacg atgtggcgac
     3661 caatcccatc atcatcggcc acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg
     3721 gcagcacaaa ttccagccgg gtcagcgtta tgtgattcag gccaacctgc aactccccga
     3781 ccgcccggat tgccccggct actccttccc gtgggtaggc ggcgaagcga cgcatgtggt
     3841 tattcccaac gaggtcatgg aacaagattg cctgctggca tacgacggcg aaacctattt
     3901 tgaaggctcg ctggttgaac cgctttcctg cgtgattggc gcgttcaacg ccaactatca
     3961 tcttcaggaa ggtagttata accacacgat ggggattcgc ccgcaagggc gcacgctgat
     4021 cctcggcggc accggaccaa tgggactgtt ggcgattgat tatgcgctac atggacccgt
     4081 taacccttcg ctgctcgtca ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa
     4141 gcactatcca tcagaaccgc aaacactgat tcattatctc aatgccgccg atgcagcatt
     4201 tgatacgtta atggcactga gtggcggtca cggcttcgat gatattttcg tctttgtgcc
     4261 taatgaagga ctggtgactc tcgcctcttc cttgctggcg acagatggtt gtctgaattt
     4321 cttcgccgga ccgcaggata agcacttcag cgcgccaatt aatttctacg atgtgcatta
     4381 tgcatttacc cactacgtgg gcacgtcagg cggcaatacc gacgacatgc gcgcagcggt
     4441 caaattaatt gaagagaaaa aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct
     4501 gaatgccgcg ggcgaaacca cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt
     4561 gtataccggg aaatacctgc cgctgacgtc actcacgcag attcaggatc aagcactggc
     4621 ggcgattctg gcgcgtcatc aggggatctg gtccggtgag gcggagcaat acctgctcgc
     4681 ccatgcagag gcaatttccc atgattaatc gcgctactta actctgcatg tcgctggctg
     4741 gtcgccgcgg caacttcgcg acctgatgtg cggctgattg ctgaagcggc acaatgtgca
     4801 cgagaaggat aaaaaaacat cagcgcctta aggggcgctc aaattggtgg caaagtgcac
     4861 attgtttatg ccggatgcgg aatgaagcct tattcggcct acataatctt gaaaattcaa
     4921 tatattgcag ttatctggta ggccaggtaa gcgtagcgca tcaggcaatc ctgtgtttgt
     4981 catcagtctc agcgcctgtg caatatgtga catcgtgagc catgtcagta agtggttaaa
     5041 ttcgcagtgg tagcattcct tccgtgtttt cttactaacc ggagaagtaa tggaactgct
     5101 tttattgagt aactcgacgc tgccgggtaa agcctggctg gaacacgcgc tgccgctgat
     5161 tgctgaacag ttgcagggtc gccgttctgc ggtatttatt cctttcgctg gcgtaacgca
     5221 gacctgggat gattacacag cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt
     5281 caccggtatt catagcgttg tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat
     5341 tgtcggcggc ggtaatactt tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc
     5401 acccattact gacgtggtga aacgtggcgc gctgtatatt ggctggagcg caggcgctaa
     5461 ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg attatcgatc cgcaaggttt
     5521 cgatgcgcta aatctgttcc cgctacaaat caacccgcac ttcaccaacg cgctgccgga
     5581 aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga
     5641 actgacgatt attggtctgc cggaaggtaa ctggatcaca gtaagtaaag gtcacgctac
     5701 gctgggtggc ccgaacacca cttatgtatt taaggctggt gaagaagcgg tgccgctgga
     5761 agcgggtcac cgtttttaag tgaattgccg gatgcatgat ccggcatctt gttcatagtg
     5821 cctgatgcaa cgctttacgc gtcttatcag gccttcaggg tcacggtatt aatactcgtc
     5881 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     5941 gttcagacgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatactctg
     6001 cacgttttgc tggtgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6061 aaaacgatgt ttgctgcggc gcaaacgacg ggcgctggta acatcgccag agaagaaaac
     6121 cgacattgcc agttttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6181 gccatcaagc gaaaatgctc gccttgctgc cagcgatttg tcggcaattt cgctgcccat
     6241 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6301 gcggcgcgac tcttcttctg ccagttcctc ttttggcatc cgcgccagat acagcttaat
     6361 ggcggtatag agaacgttga tgtcatcagc cagcttacgc agctctttct cctgccgtgg
     6421 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6481 cagggtttcg cgggcagcgt tcgccagagc aagcgtgggc gtatccagcg cgctgacatc
     6541 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6601 aaaccgtgcc atcgggtcaa cgaatggcaa catgaccagg cagcgtacaa ggttgtagaa
     6661 gacgtggaaa tagatcacca attccgcttt tggcaatggc aacttcccca ttgtctctgc
     6721 cagcaaatga acaaacggca ggataatcag gctacccacc agcttaaaca gcaggctacc
     6781 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     6841 ggaaccgagg tttgcaccaa tcaccagaca aagcgccact gggaaagaga taatgcctgc
     6901 ggcggtcaga gtcgcggtca gcagtaccgc ggcaaggctg gagtagctga taatggcgaa
     6961 catcgcacca atcagcgcat ccagcaaaat gtcgccagtc agcgaggcaa agatcacctg
     7021 aacgccgttt gcctgagtga tcggcgttac ggcctgcaca atcaactcca gcgccagtaa
     7081 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7141 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata aatcgaaggt
     7201 gagaatacgc gccatcagcg ccgtcccgac atctgcaccc agcacaatca ccagcgcagg
     7261 cgcgagagca accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7321 gctctgcacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7381 gctccggcta aggacagtac gcagacgcgc gccgaagacg cgcattacgc ctgttcgaac
     7441 aatatgggtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca aagttagcac
     7501 ggaatcaggt tctccttttt gttatgcatt ttcctgttaa gatttttcta atcacgttgt
     7561 ttttatccag gcatttctct tgtatgtact ttcagtataa gagatttata caagaaagca
     7621 tttaaaaagg catttatata aaa
//
LOCUS       DACADA010000193         7607 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.195, whole genome shotgun sequence.
ACCESSION   DACADA010000193 DACADA010000000
VERSION     DACADA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.195"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            252..1586
                     /gene="cadB"
                     /locus_tag="ISB17_004225"
     CDS             252..1586
                     /gene="cadB"
                     /locus_tag="ISB17_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAP1982096.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1666..3813
                     /gene="cadA"
                     /locus_tag="ISB17_004226"
     CDS             1666..3813
                     /gene="cadA"
                     /locus_tag="ISB17_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAP1982097.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3872..5329
                     /gene="dtpC"
                     /locus_tag="ISB17_004227"
     CDS             3872..5329
                     /gene="dtpC"
                     /locus_tag="ISB17_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HAP1982098.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5566..7083
                     /gene="lysS"
                     /locus_tag="ISB17_004228"
     CDS             5566..7083
                     /gene="lysS"
                     /locus_tag="ISB17_004228"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP1982099.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7202..7375)
                     /gene="ghoT"
                     /locus_tag="ISB17_004229"
     CDS             complement(7202..7375)
                     /gene="ghoT"
                     /locus_tag="ISB17_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HAP1982100.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7403..>7607)
                     /gene="ghoS"
                     /locus_tag="ISB17_004230"
     CDS             complement(7403..>7607)
                     /gene="ghoS"
                     /locus_tag="ISB17_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP1982101.1"
                     /translation="YDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCL
                     DLPDIDFDLSIMTVDDYFRQFYK"
BASE COUNT         1900 a         1750 c         1828 g         2129 t
ORIGIN      
        1 ttaaccagag aatgtcacgc aatgcattgt aaacattgaa tgtttatctt ttcatgatat
       61 caacttgcga tcctgatgtg ttaataaaaa acctcaagtt ctcacttaca gaaacttttg
      121 tgttatttca cctaatcttt aggattaatc cttttttcgt gagtaatctt agcgccagtt
      181 tggtctggtc aggaaatagt tatacatcat gacccggact ccaaattcaa aaatgaaatt
      241 aggagaagag catgagttct gccaagaaga tcgggctatt tgcctgtacc ggtgttgttg
      301 ccggtaatat gatggggagc ggtattgcat tattacctgc gaacctagca agtatcggtg
      361 gtattgctat ctggggttgg attatctcta ttattggtgc aatgtcgctg gcatatgtat
      421 atgcccgact ggcaacaaaa aacccgcaac aaggtggccc aattgcgtat gccggagaaa
      481 tttcccctgc atttggtttt cagacaggtg ttctttatta ccatgctaac tggattggta
      541 acctggcaat tggtattacc gctgtatctt atctttccac cttcttccca gtattaaatg
      601 atcctgttcc ggcgggtatc gcctgtattg ctatcgtctg ggtatttacc tttgtaaata
      661 tgctcggcgg tacctgggta agccgtttaa ccactattgg tctggtgctg gttcttattc
      721 ctgtggtgat gactgctatt gttggctggc attggtttga tgcagcaact tatgcagcta
      781 actggaatac tgcggatacc actgatggtc atgcgatcat taaaagtatt ctgctctgcc
      841 tgtgggcctt cgtgggtgtt gaatccgcag cagtaagtac tggtatggtt aaaaacccga
      901 aacgtaccgt tccgctggca accatgctgg gtactggttt agcaggtatt gtttacatcg
      961 ctgcgactca ggtgctttcc ggtatgtatc cgtcttctgt aatggcggct tccggtgctc
     1021 cgtttgcaat cagtgcttca actatcctcg gtaactgggc tgcgccactg gtttctgcat
     1081 tcaccgcctt tgcgtgtctg acttctctgg gctcctggat gatgttggta ggccaggcag
     1141 gtgtacgtgc cgctaacgac ggtaacttcc cgaaagttta tggtgaagtc gacagcaacg
     1201 gtattccgaa aaaaggtctg ctgctggctg cagtgaaaat gactgccctg atgatcctca
     1261 tcactctgat gaactctgcc ggtggtaaag cctctgacct gttcggtgaa ctgaccggta
     1321 tcgcagtact gctgactatg ctgccgtact tctactcttg cgttgacctg attcgttttg
     1381 aaggcgttaa catccgcaac tttgtcagcc tgatctgctc tgtactgggt tgcgtgttct
     1441 gcttcatcgc gctgatgggc gcaagctcct tcgagctggc aggtaccttc atcgtcagcc
     1501 tgattatcct gatgttctac gctcgcaaaa tgcacgagcg ccagagccac tcaatggata
     1561 accacacagc gtctaacgca cattaattaa aagtattttc cgaggctcct cctttcattt
     1621 tgtcccatgt gttgggaggg gcctttttta cctggagata tgactatgaa cgttattgca
     1681 atattgaatc acatgggggt ttattttaaa gaagaaccca tccgtgaact tcatcgcgcg
     1741 cttgaacgtc tgaacttcca gattgtttac ccgaacgacc gtgacgactt attaaaactg
     1801 atcgaaaaca atgcgcgtct gtgcggtgtt atttttgact gggataaata taatctcgag
     1861 ctgtgcgaag aaattagcaa aatgaacgag aacctgccgt tgtacgcgtt cgctaatacg
     1921 tattccactc tcgatgtaag cctgaatgac ctgcgtttac agattagctt ctttgaatat
     1981 gcgctgggtg ctgctgatga tattgctaac aagatcaagc agaccactga cgaatatatc
     2041 aacactattc tgcctccgct gaccaaagca ctgtttaaat atgttcgtga aggtaaatat
     2101 actttctgta ctcctggtca catgggcggt actgcattcc agaaaagccc ggtaggtagc
     2161 ctgttctatg atttctttgg tccgaacacc atgaaatctg atatttccat ttcagtatct
     2221 gaactgggtt ctctgctgga tcacagtggt ccacacaaag aagcagaaca gtatatcgct
     2281 cgcgtcttta acgcagaccg cagctacatg gtgaccaacg gtacttccac tgcgaacaaa
     2341 attgttggta tgtactctgc tccggcaggc agcaccattc tgattgaccg taactgccac
     2401 aaatcgctga cccacctgat gatgatgagc gatgttacgc caatctattt ccgcccgacc
     2461 cgtaacgctt acggtattct tggtggtatc ccacagagtg aattccagca cgctaccatt
     2521 gctaagcgcg tgaaagaaac accaaacgca acctggccgg tacatgctgt aattaccaac
     2581 tctacctatg atggtctgct gtacaacacc gacttcatca agaaaacact ggatgtgaaa
     2641 tccatccact ttgactccgc gtgggtgcct tacaccaact tctcaccgat ttacgaaggt
     2701 aaatgcggta tgagcggtgg ccgtgtagaa gggaaagtga tttacgaaac ccagtctact
     2761 cacaaactgc tggcggcgtt ctctcaggct tccatgatcc acgttaaagg tgacgtaaac
     2821 gaagaaacct ttaacgaagc ctacatgatg cacaccacca cctctccgca ctacggtatc
     2881 gtggcgtcca ctgaaaccgc tgcagcgatg atgaaaggca atgcaggtaa acgtctgatc
     2941 aatggttcca ttgaacgtgc gatcaaattc cgtaaagaga tcaaacgtct gagaacggaa
     3001 tctgatggct ggttctttga tgtatggcag ccggatcata tcgatacgac tgaatgctgg
     3061 ccgctgcgtt ctgacagcac ctggcacggc ttcaaaaaca tcgataacga gcacatgtat
     3121 cttgacccga tcaaagtcac cctgctgact ccggggatgg aaaaagacgg caccatgagc
     3181 gactttggta ttccggccag catcgtggcg aaatacctcg acgaacatgg catcgttgtt
     3241 gagaaaaccg gtccgtataa cctgctgttc ctgttcagca tcggtatcga taagaccaaa
     3301 gcactgagcc tgctgcgtgc tctgactgac ttcaaacgtg cgttcgatct gaacctgcgt
     3361 gtgaaaaaca tgctgccgtc tctgtatcgt gaagatcctg aattctatga aaacatgcgt
     3421 attcaggaac tggctcagaa tatccacaaa ctgattgttc accacaatct gccggatctg
     3481 atgtatcgcg catttgaagt tctgccgact atggtaatga ctccgtatgc tgcgttccag
     3541 aaagagctgc acggtatgac cgaagaagtt tacctcgacg aaatggtagg tcgtattaac
     3601 gccaatatga tccttccgta tccgccggga gttcctctgg taatgccggg tgaaatgatc
     3661 accgaagaaa gccgtccggt tctggagttc ctgcagatgc tgtgtgaaat cggcgctcac
     3721 tatccgggct ttgaaaccga tattcacggt gcataccgtc aggctgatgg ccgctatacc
     3781 gttaaggtat tgaaagaaga aagcaaaaaa taattagctc gtacaaggga agtggcttgc
     3841 cacttccctt ttttgctcat aaggagaaca catgaaaaca ccctcacagc cgcgcgcgat
     3901 atactatatc gtggcgatcc aaatctggga gtacttcagt ttttacggca tgcgtgcctt
     3961 actcattctc tatctcaccc atcagcttgg ttttgatgat aaccatgcca tcagcctgtt
     4021 cagcgcatat gcttctctgg tttacgttac ccctattctc ggcggctggc ttgccgaccg
     4081 cctgctcggc aaccgcactg cagtgattgc cggcgcgctg ttaatgaccc ttggccatgt
     4141 ggtgctgggc attgatacaa attcaacctt tagcctgtat ctggcgctgg caatcattat
     4201 ttgtggctac ggtttattca aatcaaacat cagctgtttg cttggcgagc tctacgacga
     4261 gaacgatcat cgacgtgatg gcggtttttc gctgctgtat gctgcgggca atatcggttc
     4321 tatcgccgcg cccatcgcct gcggcctggc tgctcagtgg tatggatggc atgttggctt
     4381 tgcccttgcg ggaggcggca tgtttatcgg tttgttgatt ttcttaagtg gtcatcgtca
     4441 tttccagtcc acgcgtagta tggataaaaa agcgctcact agcgtcaaat ttgccttacc
     4501 agtatggagc tggttagtgg tgatgctctg tttagcccca gtatttttta ccctgctgct
     4561 ggagaacgac tggtccggat atttgctggc gatcgtttgc ctcattgcag cacaaatcat
     4621 tgcccgcatg atgatcaaat tccccgaaca ccgccgtgct ctttggcaaa ttgtattgtt
     4681 gatgtttgtc gggacgttgt tctgggtact ggcacaacag ggcggcagta ccatcagctt
     4741 gtttatcgat cgctttgtga atcgtcaggc attcaatatt gaagtaccta cagcactatt
     4801 ccagtcggtg aatgctattg cggtgatgct cgctggggtt gttctggcct ggctggcgtc
     4861 gccagaaagc cgcggcaatt cagcattgcg cgtctggctg aagtttgcct ttggcttact
     4921 gctgatggct tgtggcttta tgttgctggc atttgatgcc cgacatgcag cggctggcgg
     4981 tcaagcgtca atgggcgtga tgatatccgg gctggcgcta atgggctttg ctgaactctt
     5041 tattgacccg gtggcgattg cgcaaatcac gcgtctgaaa atgtctggcg tattaaccgg
     5101 tatttatatg ctggcaacag gcgcggtcgc caactggctg gcaggcgtcg tggcacagca
     5161 gacgacagag tcgcaaatta gcggtatggc aattgccgct taccagcgat tcttttctca
     5221 gatgggagag tggacgttgg cttgtgtcgc gatcatcgtg gtattggcct ttgctacccg
     5281 ttttctgttt agcacgccga cgaatatgat acaggagagc aacgattaac tgtcgtaaaa
     5341 acaattggtt atgaataaat taactttcag tttatcacac tagtaaatcg agattaataa
     5401 gtcatttaaa ccattttgat ggttatttat tagtgatatc aacttgaggt aagcgttagt
     5461 ttcgataaga taaactgagt tattaatagt cgaggcagat aatacagtgt accgatctgt
     5521 ctctttatct acgctaaatt gaaagctgga tttagaggaa ccaaaatgtc tgaacgagaa
     5581 acacggggag ccaatgaggc tattgatttt aacgatgaac tgagaaatcg ccgcgaaaaa
     5641 ctggcggcac tacgtcagca aggtgtggcg tttcccaatg attttcgccg cgaccatacc
     5701 tctgaccagt tgcacgaaga gtttgatgcg aaggataacc aggaactgga atccttaaac
     5761 attgaagtct cggttgctgg ccgaatgatg acccgtcgta ttatggggaa agcctccttt
     5821 gtaacgttgc aggatgtcgg tggccgtatt caactgtacg ttgcaagaga tagcctgcca
     5881 gaaggtgttt ataacgatca gtttaaaaaa tgggatctgg gtgacattat cggtgcccgc
     5941 ggtacgctgt ttaagacgca aacgggtgag ctttccattc actgtactga gctgcgcctg
     6001 ctgactaaag cactacgtcc tttaccagat aaattccatg gtctgcagga tcaggaagtc
     6061 cgttatcgtc aacgttatct ggacctcatc gctaacgata aatcccgtca aacgtttgtt
     6121 gtccgttcaa aaattctggc cgctatccgt cagttcatgg tcgcgcgcgg ctttatggaa
     6181 gtagaaaccc cgatgatgca ggtaattcca ggtggggcat ctgctcgccc gtttattacc
     6241 catcataacg ctctggattt agatatgtac ctgcgtatcg cgccggagct gtatctgaaa
     6301 cgtctggttg taggcggttt tgaacgggta ttcgaaatca accgtaactt ccgtaatgaa
     6361 ggtatttctg ttcgccataa tcctgagttc acaatgatgg aactctacat ggcatatgcg
     6421 gattaccatg atttgattga actgacggag tcactgttcc gcaccctggc acaagaggta
     6481 ctgggtacca ctaaagtcac ttatggcgag catgtgtttg atttcggcaa accgtttgaa
     6541 aaactcacca tgcgcgaagc aatcaaaaaa tatcgtccag aaaccgatat ggcggatctg
     6601 gataattttg atgctgctaa agcattagct gaatctatcg gtattacggt agagaaaagc
     6661 tggggactgg ggcgtattgt tacggagatc tttgatgaag tggcagaagc acatctgatt
     6721 cagccaacct ttattacgga atatccggca gaagtgtccc cgctggcgcg ccgtaatgat
     6781 gttaacccgg aaatcaccga ccgttttgaa ttcttcatcg gtggtcgtga aattggtaat
     6841 ggttttagcg aattaaacga cgcagaagat caggctgaac gtttccagga acaggttaac
     6901 gctaaagctg caggtgacga cgaagccatg ttctatgacg aagattacgt gactgcgctg
     6961 gaatatggtc tgccgccaac cgctggtctg ggtattggta tcgaccgaat gattatgctg
     7021 tttacaaaca gtcatactat tcgcgacgtt attctcttcc cggcgatgcg cccacagaaa
     7081 taaatttcac tttaatgaac gaagcagtca ggcgactgct tcgttcgtca ttccgcaaat
     7141 ccagttttga ggagagacgc ttcgtttcaa gataaccagc gggtccactc aactacagct
     7201 cctaaaagag agaaaaaagt aatgccacag gcaaactcat tggccaggtt attccgacca
     7261 gaaatgcact aagtaaacga atatgtgttt tctcatgtga gataaaccag ataatgacaa
     7321 aggatatgtt cacaccaatc acataaaaaa ttaatatttt agagaatagt gccatttttt
     7381 atgttcttct tatccttcct ggctacttgt aaaactgacg gaaatagtca tcaactgtca
     7441 taatgctgag gtcaaaatca atatccggta agtctaagca tttgagcctg attaaatctt
     7501 cgatttcact tgcggctaac gatgtcgtta ttgcgaagga gttttcattc aattgtcgtg
     7561 gtatcccata ttcatcagcc acgatagagg agaaattcat atcatac
//
LOCUS       DACADA010000194         7552 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.196, whole genome shotgun sequence.
ACCESSION   DACADA010000194 DACADA010000000
VERSION     DACADA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.196"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1690
                     /gene="traD"
                     /locus_tag="ISB17_004231"
     CDS             <1..1690
                     /gene="traD"
                     /locus_tag="ISB17_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAP1982102.1"
                     /translation="VRARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWK
                     ECLTLPDFDNISNTLIPMGTKEDPFWQGSGRTIFAEGAYLMREDKDRSYEKLVDTMLS
                     IKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEKNGEPFTIRD
                     WMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWIFADE
                     LPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRS
                     PSKEIAEFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPD
                     LSCYVTLPGPYPAVKLALKYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLAR
                     ALFTPDAPASGPADTNSHAGEQPEPVSQPAPADMTVSPAPVKAPPTTKMPAEEPSVRA
                     TEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTEQELAQQSAEQGQDMLP
                     AGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF"
     gene            1690..6951
                     /gene="traI"
                     /locus_tag="ISB17_004232"
     CDS             1690..6951
                     /gene="traI"
                     /locus_tag="ISB17_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAP1982103.1"
                     /translation="MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGID
                     GKAVDKALFTELLKGKLPDGSDLTRIQDGANKHRPGYDLTFSAPKSVSVMAMLGGDKR
                     LIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNLIVAKFNHDTNRNQEPQIHTH
                     AVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVEKLGYETEV
                     VGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMV
                     EWMNTLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKV
                     QFTYADLLARTVGQLEAKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDEL
                     AVKALSQEVQRQNHVSVTPDAFVVRQVPFSDAVSVLAQDRPVMGIVSGQGGATGQRER
                     VAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTGKSALQDGTAFIPGGTLI
                     VDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYRWQGGH
                     QTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEG
                     VLGEKDTTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNM
                     LTLKDREGVRLDLKVSAVDSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVM
                     KVEDGQLTVQRPGQKTTQTLAMGAGVFDGIKIGHGWVESPGRSVSETATVFASVTQRE
                     LDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQLKTRSGETDLDAAIA
                     QQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTIQAQI
                     RSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLT
                     AGQRAATRMILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHR
                     AVGEMQSAGVDARTTASFLHDTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSL
                     IVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQRSAADVAIMKEIVRQMPELRPAVYSLI
                     ERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEKALSEGKTLPEGQP
                     ATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLPV
                     LVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISP
                     REASAEGVTLYRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKL
                     TRTLTPKADQAQQHIDLAYAITAHGAQGASEPYAIALEGVAGGREQMASFESAYVALS
                     RMKQHVQVYTDNREGWIKAIKHSPEKATAHDILEPRNDRAVKTADLLFGRARPLDETA
                     AGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIWLSPLTDRDGRL
                     EAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
                     PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAR
                     EVAGEARKAGEPADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASE
                     RLQRERLQAVERDMVRDLNREKTLGGD"
     gene            7032..>7552
                     /gene="traX"
                     /locus_tag="ISB17_004233"
     CDS             7032..>7552
                     /gene="traX"
                     /locus_tag="ISB17_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAP1982104.1"
                     /translation="MRAQHTFPGATDSWLQRLLTWTPGQTDMIKTVALVLMVTDHTGL
                     LLAGNNEVMRLLGRGCFPLFGLAWGMNLARHAEIRQSQLNSLWGWALVAQVSFMLIGY
                     PWYTGNILFAFAVTGQVLRWVSQPSWYYTLPAAGLLVTWIPLSTGSYGMAGVGMLTAS
                     WLLCRAQHAPERLG"
BASE COUNT         1663 a         2114 c         2495 g         1280 t
ORIGIN      
        1 tgttcgtgcc cggggcgata tggccatcat ttacgatcgc tcctgtgagt tcgtcaaaag
       61 ctattacgac ccgtcactgg ataaaatcct caacccgctg gactcgcgct gcgcggcctg
      121 ggatttgtgg aaagagtgcc tgacgctgcc ggacttcgac aacatctcca acactcttat
      181 cccgatgggc accaaagaag acccgttctg gcagggctcc ggtcgcacta tctttgccga
      241 aggcgcgtac ctgatgcgtg aagacaagga tcgcagctac gagaagctgg ttgataccat
      301 gctgtccatt aaaatcgaca agctgcgcgc ctatctgcag aacacgccgg cagcgaacct
      361 ggttgaagag aaaattgaga aaacggccat ctccattcgt gccgtgctga ccaactacgt
      421 caaggctatt cgctacctgc agggcatcga gaagaacggg gagccgttca ctatcaggga
      481 ctggatgcgc ggtgtccgtg aggaccgacc caatggctgg ctgtttatct cctccaacgc
      541 tgacacccat gcttccctga agccggttat ctcgatgtgg ctgtcaattg ctatccgcgg
      601 cctgctggcg atgggggaga accgtaaccg gcgcgtgtgg attttcgccg acgagctccc
      661 cacgctgcac aagctgccgg acctggtcga aatcctcccg gaggcgcgta aatttggtgg
      721 ttgttatgtg ttcggtattc agtcctatgc ccagctggaa gatatctatg gcgtcaagcc
      781 tgccgccacg ctctttgacg tgatgaacac ccgagccttc ttccgctccc cgagtaaaga
      841 gattgcggag tttgctgccg gtgaaattgg cgagaaggaa atcctcaagg ccagcgagca
      901 gtactcctac ggtgctgacc cggtgcgtga tggtgtatcg accgggaaag aaaaagagcg
      961 ggaaacgctg gtcagttact ccgatattca gacgctgcct gacctgtcct gctatgtcac
     1021 gctgcccggg ccgtatccgg ccgtgaaact ggccctcaaa tacaaaccgc gtccgaaaat
     1081 tgccgaggga tttattcctc gcactctcga tgcgcgggtg gatgcgcggc tcagtgcgtt
     1141 gcttgaagcg cgggaagctg aaggcagtct tgccagggca cttttcacgc cggatgcgcc
     1201 ggcatccggg cccgctgata caaacagtca cgccggtgaa caacctgagc cggtcagtca
     1261 gcctgcgcca gcagacatga cagtatctcc ggcaccggta aaagcgccgc cgacaacgaa
     1321 gatgccggca gaggaacctt ccgtcagagc aacggagcct tccgtcctgc ggctcacgac
     1381 cgtaccgttg atcaagccaa aagcagccgc tgctgccgct gcagccgcaa cagcatcctc
     1441 tgccggtacc cctgctgcag ctgcaggcgg gacagaacag gagctggcgc agcagtctgc
     1501 ggaacaggga caggacatgc tgccggccgg catgaacgag gacggtgtca tcgaggacat
     1561 gcaggcgtat gacgcctggc tggcagatga gcagacacag cgggatatgc agcggcgtga
     1621 ggaagtcaat atcaaccaca gccaccgcca cgatgaacag gattatgtcg agattggggg
     1681 gaacttctga tgatgtcgat tggctccgtt aaatcggcgg gcagtgccgg taattactat
     1741 acggataagg ataattacta cgtcataggg agcatggacg aacgctggca gggtaagggg
     1801 gctgaggcgc tggggatcga cgggaaagcc gtggataagg cgctgtttac ggaactgctg
     1861 aaagggaagc tgccggatgg cagcgacctg acccggatac aggacggcgc caacaagcac
     1921 cggcccggtt atgacctgac cttctcggcc cccaaaagcg tgtcggtcat ggcgatgctg
     1981 ggcggcgata aacgccttat cgatgcccac aaccaggcgg taaccgaagc ggtgcggcaa
     2041 ctggagacgc tggccgcgac ccgggtcatg acggacggta aaagcgaaac ggtgctgacc
     2101 ggaaatctga ttgtggcgaa attcaaccac gacacgaacc gtaaccagga acctcagata
     2161 cacacccatg ccgtcgtcat taacgccacg cagaacgggg acaaatggca gtctctcggc
     2221 acagataaga tcggtaaaac aggttttatc gagaacgtgt acgcgaacca gatagcgttc
     2281 gggaagcttt accgggaggc gttcaaaccc ctggttgaga agcttggcta tgagacggaa
     2341 gttgtgggca aacacgggat gtgggagatg aaaggggtcc ctgtggaacc gttctccacc
     2401 cggtctcagg aggtcaggga ggccgcaggg cccgatgcct ccctgaagtc ccgggatgtg
     2461 gccgcgctcg atacccgcaa gtcgaaagac gccatcgatc cggcagagaa gatggtggag
     2521 tggatgaaca cgctgaagga gaccgggttt gacatccggg ggtaccgtga ggccgccgat
     2581 gcgcgagccg cagagctggc ccgtgcacct gccgcgccgg tgaacactga cggtccggat
     2641 atcacggatg tggtgacgaa agcgattgcc ggtctcagcg accgcaaggt gcagttcacg
     2701 tatgccgacc tgctggcacg cacggtcggc cagctggagg cgaaggacgg tgtgtttgag
     2761 ctggcgagaa agggcatcga tgccgccatt gaacgggagc agcttatccc tctcgatcgc
     2821 gagaaaggcc tgttcacctc aaacatccac gttctcgatg aactcgccgt caaagccctt
     2881 agtcaggagg tgcagcgcca gaatcatgtc tccgtcacgc ctgatgcgtt tgtggtgcgc
     2941 caggtccctt tcagcgatgc cgtcagtgtg ctggcgcagg atcgcccggt aatgggtatc
     3001 gtttccggtc agggcggtgc caccggccag cgggagcggg tggccgagtt aaccctgatg
     3061 gcccgggagc aggggcgtga cgtccatatc ctggcggccg ataaccgctc acgtgatttt
     3121 ctcgccggtg atgtgcgtct ggccggggaa acggtgaccg gtaaatccgc cctgcaggac
     3181 gggacggcgt ttattccggg cggaacactc atcgtggatc aggcggagaa gctgagcctg
     3241 aaggaaacga tttccctgct cgatggtgcg atgcgccata acgtgcaggt cctgctctcc
     3301 gacagcggga agcgcagcgg gaccggcagc gcgctgacgg tgctgaagga cagcggcgtg
     3361 aatacgtatc gctggcaggg tggccatcag accacagccg acatcatcag tgaaccggat
     3421 aaaggagcac gctacagccg gctggcacag gagtttgccg tcagcgtgcg tgaagggcag
     3481 gaaagcgtgg cccaaatcag cgggacgcgt gaacagagcg tactcaacgg ccttatccgt
     3541 gactccctca gacaggaggg ggtgctgggt gagaaagaca cgaccattac agccctgacg
     3601 ccggtgtggc tggacagcaa aagccggggc gtgcgggatt attaccggga ggggatggtg
     3661 atggaacgct gggatccgga gacccggacg cacgatcgct ttgtcattga ccgggtgacc
     3721 gggagcagca atatgctgac cctgaaagac cgggaagggg tccgtctgga tctgaaggtt
     3781 tccgcggtcg acagccagtg gacgctgttc cgggcagata cactgccggt ggcagagggg
     3841 gaacgtctgg cggtgctcgg gaagataccg gacacacgcc tgaagggcgg ggaaagcatc
     3901 acggtgatga aagtggagga cgggcagctg acggtacagc ggccggggca gaaaaccacg
     3961 cagaccctgg ccatgggcgc gggcgtgttt gacggtatta aaatcggcca cggctgggtg
     4021 gaaagtcccg gcaggtcggt tagcgagacg gcaacggtct ttgcgtcggt gacgcagcgt
     4081 gagctggata acgccaccct caaccagctg gcgcagagcg gaagtcatct ccggctgtac
     4141 tcggcgcagg atgcggcgcg cacgacggag aagctgtcgc ggcataccgc cttcagcgtg
     4201 gtctcagagc agctgaagac ccgatccggc gagacggacc tggatgcggc cattgcgcag
     4261 cagaaggccg gactgcgtac cccggcagaa caggccattc acctggccat cccgctgctt
     4321 gaaagtgaga aactgacctt ctcccggccc cagctgctgg cgacggcgct ggaaaccggg
     4381 ggcgggaagg tttcgatggc ggacatcgac acgaccatcc aggcacaaat caggtcgggg
     4441 cagttgctga atgtaccggt ggcgcacggc tacggcaatg acctgctgat ttcccgtcag
     4501 acctgggacg ccgaaaaaag catcctgacc cacgtgctgg aaggtaaaga cgcggtggcc
     4561 cccctgatgg accgcgtgcc ggccagtctg atgacggacc tgaccgccgg acagcgggcg
     4621 gcgacccgga tgattctgga aagcacggac cggttcacgg tggttcaggg gtatgccggg
     4681 gtggggaaaa ccacccagct ccgggcagtc atgagtgcca tcagtctgct gccggaagag
     4741 acccgtccgc gcgtcatcgg tctggcgccg acgcatcgcg cggtcgggga aatgcagagt
     4801 gctggcgtgg atgcgcgcac gacggcatcg ttcctccacg acacgcagct gctacagcgc
     4861 aatggccaga cacccgattt cagcaacacc ctgttcctgc tcgacgagag ctcaatggtg
     4921 gggctggccg atatggcaaa agcccactcc ctcatcgtcg ccggcggtgg gcgggcggtg
     4981 tccagtgggg ataacgacca gctgcagccc atcgcgccgg gccagccgtt caggctgacg
     5041 cagcagcgca gcgccgccga cgttgccatc atgaaagaga ttgtacgcca gatgcctgag
     5101 ctgcgaccgg cggtctacag cctgattgag cgggatgtgc accgcgccct gaccaccatc
     5161 gagcaggtca cgccggagca ggtgccgaga aaagaaggcg cctgggcgcc gggcagttct
     5221 gtggtggaat tcacgccaaa gcaggaaaag gcgatagaaa aggcgctgag cgaggggaaa
     5281 accctgccgg aaggtcagcc cgcgacgctc tacgaggcgc tggtgaagga ctataccgga
     5341 cggaccccgg aggcacagtc gcagacgctg gtgatcaccc atctgaataa ggaccggcgg
     5401 gcgctgaaca gtctgatcca tgacgcacgg cgggagaacg gggaaaccgg caaagaggaa
     5461 atcaccctgc cggtgctggt cacgagtaac atccgtgacg gtgaactgcg caaactgtcg
     5521 acctggacgg ctcacaagga ggcggtggcg ctggttgata acgtatacca ccgtatcagc
     5581 aaggtggata aggctaatca gctgatcacc ctgacagaca gtgaaggaaa ggagcgatat
     5641 atctcacccc gggaggcatc ggcagaaggc gtcacgctct atcgtcagga aaagataacc
     5701 gtgtctcagg gggaccgaat gcgcttcagc aaaagtgacc tggagcgcgg ctatgtggcc
     5761 aacagtatct gggaggtgca gtctgtttcg ggtgacagtg tcacgctatc tgatggcaaa
     5821 ctcacccgta cgctgacccc gaaggcggat caggcgcagc agcatatcga cctcgcctac
     5881 gccattacgg cccacggcgc ccagggggcc agcgaaccgt atgccatcgc gctggaaggg
     5941 gtggccggtg gtcgggaaca gatggccagc tttgagtctg cctatgtggc gttatcccgc
     6001 atgaaacagc atgttcaggt ctacaccgac aaccgtgagg gctggataaa ggccatcaag
     6061 cattcacctg agaaagcgac ggcgcacgat attcttgaac cccgtaacga ccgggcggtg
     6121 aaaacagccg atctgctgtt cgggcgggca aggccactgg atgaaaccgc cgcgggccgg
     6181 gcagccctgc agcagtccgg tctggcacag ggaagttcac caggaaaatt catttctccg
     6241 gggaaaaagt accctcagcc gcatgtggcg ctgccggcat ttgataaaaa cgggaaagcc
     6301 gccggtatct ggctcagccc gctgaccgat cgggacggac ggctggaggc tatcggcgga
     6361 gagggacgaa ttatgggcaa tgaagacgcc cggttcgtgg cgctgcagaa cagccgcaac
     6421 ggagaaagcc tgctggccgg gaatatgggg gagggcgtca gaatggcccg cgacaatccc
     6481 gatacggggg tggtggtgcg gctggccggt gatgaccgcc cctggaaccc cggggccatg
     6541 accggtgggc gggtatgggc tgaaccggcg ccggtggctc cggtaccgca ggccggggct
     6601 gacattatcc tcccgccaga ggtactggca cagcgggcgg cagaagaaca gcagcgccgg
     6661 gaaatggaaa aacaggcgga acagactgcc cgggaagtgg ccggggaggc gcgtaaagcc
     6721 ggcgagccgg cggaccgggt gaaagaggtt atcggggacg tcattcgcgg actggagcgt
     6781 gaccggccgg gtacggagaa aacgacgcta ccggatgatc ctcagttccg gcgccaggag
     6841 gaggcgattc agcaggtggc ctcagaacgt ctgcagcgtg agcgtctgca ggctgtggag
     6901 cgggacatgg ttcgtgacct taaccgcgag aaaacactcg ggggcgactg acgcgcctgc
     6961 ggcttgtctg ctccggcagc ctccccgatt gaaatcgggc agtctgccgg agcgctaaaa
     7021 ggaaaaaaaa catgagagca caacacacat ttccaggcgc gacagacagc tggcttcagc
     7081 gcctgctgac ctggacaccg ggacaaacgg atatgattaa aacggtcgca ctggtactga
     7141 tggtgactga ccacaccggc ttactgctgg ccggaaacaa tgaagtgatg cgcctgctgg
     7201 gacgtggttg ttttccgctg ttcggtctgg catggggaat gaatctggca cggcacgcgg
     7261 aaatacgcca gtctcaactg aacagcctgt ggggctgggc gctggtcgcc caggtgtcgt
     7321 ttatgctcat cgggtacccg tggtataccg ggaatattct gtttgccttt gccgttacgg
     7381 gccaggtcct gcggtgggtc agccagccgt cctggtatta caccctcccg gcggctggac
     7441 tgctggtcac ctggataccc ctgtcaaccg gcagctacgg catggccgga gtgggcatgc
     7501 tcaccgccag ctggctgctt tgtcgcgcac agcatgcacc ggaacggctg gg
//
LOCUS       DACADA010000195         7493 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.197, whole genome shotgun sequence.
ACCESSION   DACADA010000195 DACADA010000000
VERSION     DACADA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.197"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..205
                     /gene="ydgT"
                     /locus_tag="ISB17_004234"
     CDS             <1..205
                     /gene="ydgT"
                     /locus_tag="ISB17_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAP1982105.1"
                     /translation="DYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRA
                     ELVSGGRLFDLGQVPKSVWHYVQ"
     gene            291..731
                     /locus_tag="ISB17_004235"
     CDS             291..731
                     /locus_tag="ISB17_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAP1982106.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            808..1389
                     /gene="rsxA"
                     /locus_tag="ISB17_004236"
     CDS             808..1389
                     /gene="rsxA"
                     /locus_tag="ISB17_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAP1982107.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1389..1967
                     /gene="rsxB"
                     /locus_tag="ISB17_004237"
     CDS             1389..1967
                     /gene="rsxB"
                     /locus_tag="ISB17_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAP1982108.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            1960..4380
                     /gene="rsxC"
                     /locus_tag="ISB17_004238"
     CDS             1960..4380
                     /gene="rsxC"
                     /locus_tag="ISB17_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAP1982109.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANA
                     EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAA
                     QQKVVNED"
     gene            4381..5439
                     /gene="rsxD"
                     /locus_tag="ISB17_004239"
     CDS             4381..5439
                     /gene="rsxD"
                     /locus_tag="ISB17_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAP1982110.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5443..6063
                     /gene="rsxG"
                     /locus_tag="ISB17_004240"
     CDS             5443..6063
                     /gene="rsxG"
                     /locus_tag="ISB17_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAP1982111.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            6067..6762
                     /locus_tag="ISB17_004241"
     CDS             6067..6762
                     /locus_tag="ISB17_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAP1982112.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            6762..7397
                     /gene="nth"
                     /locus_tag="ISB17_004242"
     CDS             6762..7397
                     /gene="nth"
                     /locus_tag="ISB17_004242"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP1982113.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1741 a         1945 c         1995 g         1812 t
ORIGIN      
        1 ggactactta ttaaaatttc gcaaaatcag ttcactcgaa agtctggaaa aactctacga
       61 ccatcttaat tacaccctga cggacgatca ggaactgatc aatatgtatc gtgctgccga
      121 tcaccgtcgc gcagagctgg tttctggcgg gcgtttgttt gacctcggcc aggtaccgaa
      181 gtccgtctgg cactatgtcc agtaaagaaa gtagcgatcg gttataaaag ccttataata
      241 acgcccctaa aattagattt gtctgtttgc gtcgctattc tggagaacct atgaccacca
      301 caacgccaca acgtattgga ggctggttgc ttggcccgtt ggcctggctt ttagtcgctc
      361 tgttgagtac gacgctggcg ttgttgctgt ataccgctgc gttatcttct ccgcaaacat
      421 ttcaaacgct cggcggacaa gcactgacga cacaaatctt atggggcgtt tcttttatta
      481 ccgctatcgc catgtggtat tacacactgt ggttgaccat cgctttcttt aaacgtcggc
      541 gttgtgttcc taaacactat attatctggc tgcttatttc cgtattgctg gcagtaaaag
      601 cgttcgcttt ttcacccgta gaagatggca tagccgttcg ccagttgctg tttactttgt
      661 tggcaaccgc attgatcgtc ccctatttca aacgctcgtc gcgggtgaaa gcgacgtttg
      721 tgaatccgta ataaccttac agttaacctg ttgtcgcctg ctctggatta acggataata
      781 ggcggctttt ttatttcagg ccgaaaaatg actgactacc tgttactgtt tgtcggaact
      841 gtactggtca ataactttgt actggtcaag tttctcggtc tctgtccgtt tatgggggtt
      901 tccaaaaagc tggaaaccgc gatgggcatg gggctggcaa caacgtttgt aatgacgctg
      961 gcgtctattt gcgcctggct tatcgatacg tggattttga tcccacttaa tctgatttat
     1021 ctgcgcaccc tggcgtttat tctggtgatt gctgtggttg tgcagttcac cgaaatggtg
     1081 gtgcgcaaaa ccagcccggt actttaccgt ctgctgggga tttttttgcc gcttatcacc
     1141 accaactgtg ccgtgctcgg cgtggcgttg ctgaatatca atctcgggca caatttcttg
     1201 cagtcagcgc tgtacggttt ttccgccgct gtcggtttct cgctggtgat ggtgctcttc
     1261 gccgccatcc gcgaacgcct tgctgtggct gatgtcccgg caccttttcg cggtaatgcc
     1321 attgcgttaa ttaccgcagg tcttatgtct ctggccttta tgggctttag tggtttggtg
     1381 aagttgtaat gaatgctatc tggattgccg ttgccgccgt gagcctgctg gccctggcgt
     1441 ttggcgccat tctgggttat gcctcccgcc gttttgcggt ggaagacgat ccggtcgttg
     1501 agaaaattga cgaaatctta ccgcagagcc agtgtggtca gtgcggttat cccggctgtc
     1561 gcccctacgc ggaagccatc agctgtaacg gtgaaaaaat caaccgttgc gccccaggtg
     1621 gcgaagctgt gatgctaaaa attgccgagt tgcttaatgt cgagccgcag ccgctggatg
     1681 gcgaagcgca agagctaacg ccagcgcgga tggtggcggt tattgatgaa aataactgta
     1741 ttggctgcac taaatgtatt caggcgtgtc cggtagacgc catcgttggc gctacccgcg
     1801 ccatgcatac ggtaatgagt gatctctgta cgggctgcaa tttatgtgtt gatccgtgcc
     1861 cgacgcactg catctcgttg caaccggtcg cagaaactcc tgactcctgg aaatgggatc
     1921 tgaacaccat tcccgtgcgt atcattcccg tggaacacca tgcttaagtt attctctgca
     1981 ttcagaaaaa ataaaatctg ggatttcaac ggcggcatcc atccaccgga gatgaaaacc
     2041 cagtccaacg gtacgcccct gcgccaggta ccactggcgc agcgttttgt cattccacta
     2101 aaacaacata ttggcgctga aggtgagttg tgcgttagcg tcggcgataa agtattgcgc
     2161 ggccagccgc ttacccgtgg tcgcggcaaa atgctgccgg ttcacgcgcc cacctcgggt
     2221 accgttacgg ctattgcgcc ccactctacg gctcatcctt cagctttagc tgaattaagt
     2281 gtgattattg atgccgatgg cgaagactgc tggatcccgc gcgacggctg ggccgattat
     2341 cgctctcgca gtcgcgaaga gttaatcgag cgcattcatc agtttggtgt tgccgggctg
     2401 ggtggtgcgg gatttccgac aggcgttaaa ttgcagggcg gcggagataa gattgaaacg
     2461 ttgattatca acgctgctga gtgcgagccg tacattaccg ccgatgaccg tttgatgcag
     2521 gattgcgcgg ctcaggtcgt agagggtatc cgcatccttg cgcatattct gcagccgcgc
     2581 gaaattctta tcggcattga agataacaaa ccgcaggcga tttccatgct acgcgcggtg
     2641 ctggcggact cccacgatat ttctctgcgg gtgattccca ccaaatatcc ttctggcggt
     2701 gctaaacaat taacctacat tctgaccggg aagcaggttc cacatggcgg gcgttcatcc
     2761 gatatcggcg tattaatgca aaacgtcggc accgcttatg cagtgaaacg tgccgttatt
     2821 gatggcgagc cgattaccga gcgtgtcgtg accctgactg gcgaagcaat cgctcgcccg
     2881 ggcaacgtct gggcacggct gggtacgcca gtgcgtcatt tattgaatga tgccggattc
     2941 tgcccctctg ccgatcaaat ggtgattatg ggcggcccgc taatgggctt taccttaccg
     3001 tggctggatg tcccggtcgt aaagattacc aactgtcttt tggctccctc tgccaatgaa
     3061 cttggcgaac cacaggaaga acaaagttgc atccgttgta gcgcctgtgc tgacgcctgc
     3121 cctgcggatc ttttgccgca acagctgtac tggttcagca aaggtcagca acacgataaa
     3181 gccaccacgc ataacattgc cgactgcatt gaatgtgggg cttgcgcgtg ggtttgcccg
     3241 agcaatatcc ccctggtgca atatttccgt caggaaaaag ctgaaattgc ggctattcgt
     3301 caggaagaaa agcgcgccgc agaagccaaa gcgcgtttcg aagcgcgcca ggctcgtctg
     3361 gagcgcgaaa aaacggctcg ccttgaacga cataagagcg cagccgttca acctgcagcc
     3421 aaagataaag atgcgattgc tgccgctctg gcgcgggtga aagagaaaca ggcccaggct
     3481 acacagccta ttgtgattaa agcgggcgaa cgcccggata acagtgcaat tattgcagca
     3541 cgggaagccc gtaaagcgca agccagagcg aaacaggcag aactgcagca aactaacgac
     3601 gctgcaaccg ttgctgatcc acgtaaaact gccgttgaag cggctattgc ccgcgccaaa
     3661 gcacgcaagc tggagcagca acaggccaat gcggagccag aacaacaggt tgatccgcgc
     3721 aaagccgccg tcgaagccgc tattgcccgt gccaaagcac gcaagctgga acagcaacag
     3781 gctaatgccg aaccagaaca acaggtcgat ccgcgcaaag ctgccgtcga agcggctatt
     3841 gcacgcgcca aagcgcgcaa gctggaacag caacaggcca atgcggaacc agaagaacag
     3901 gttgatccac gcaaagccgc cgtcgaagct gctattgccc gtgccaaagc acgcaagctg
     3961 gaacagcaac aacaggctaa tgcggaacca gaagaacagg ttgatccacg caaagccgcc
     4021 gtcgaagccg ctatcgcccg cgccaaagca cgcaagctgg aacagcaaca ggccaatgcg
     4081 gagccagaac aacaggtcga tccgcgcaaa gctgccgtcg aagcggctat tgcacgcgcc
     4141 aaagcgcgca agctggaaca gcaacaacag gctaatgcgg aaccagaaga acaggttgat
     4201 ccacgcaaag ccgccgtcga agctgctatt gcccgtgcca aagcacgcaa gctggaacag
     4261 caacaggcta atgcagagcc agaagaacag attgatccgc gcaaagcggc agttgctgcg
     4321 gctattgccc gcgttcaggc caaaaaagcc gcccagcaaa aggttgtaaa cgaggactaa
     4381 atggtattca gaatagctag ctccccttat acccataacc agcgccagac atcgcgcatt
     4441 atgatgttgg tattgctcgc agccgtgcca ggaatcgcag cgcaactgtg gttttttggt
     4501 tggggtactc tcgttcagat cctgttggcg tcggtcagtg ctcttctggc cgaagctctc
     4561 gtcctcaaac tacgcaagca gtcggtagct gcaacgttga aagataactc agcattgctg
     4621 acaggcttat tgctggcggt aagtattccc cccctcgcgc catggtggat ggtcgtgctg
     4681 ggtacggtgt ttgcggtgat catcgctaaa cagttgtatg gcggtctggg gcaaaacccg
     4741 tttaatccgg caatgattgg ttatgtggtc ttactgatct ccttcccggt gcagatgacc
     4801 agctggttac cgccacatga aattgcggtc aacatccctg gttttatcga cgccatacag
     4861 gttattttca gcggacatac caccagtggt ggtgatatga acacgctacg cttaggtatt
     4921 gatggcatta gtcaggcgac accgctggat acatttaaaa cctctgtccg tgccggtcat
     4981 tcggttgaag agattatgca atatccgatc tacagcggta ttctggcggg cgctggttgg
     5041 caatgggtaa atctcgcctg gctgactggc ggcgtgtggt tgctatggca gaaagcgatt
     5101 cgctggcata ttcccctcag cttcttagta acgctggcgt tatgcgcaac gttgggttgg
     5161 ttgttctcac cagatactct ggcagcaccg caaattcatc tgctgtctgg tgcgaccatg
     5221 ctcggcgcat tctttatttt gactgacccg gttaccgctt ctacgaccaa tcgtggtcgt
     5281 cttattttcg gcgcgctggc gggcttatta gtctggttga tccgcagttt cggcggctat
     5341 cctgacggcg tggcttttgc cgtcctgctg gcgaacatca cggttcctct gatcgattac
     5401 tacacgcgtc cgcgcgtcta cggccatcgc aaagggtaaa ccatgctgaa aactatccga
     5461 aaacacggca ttacgttggc gctctttgca gcgggttcaa cagggttaac tgcggccatc
     5521 aaccagatga ccaaaacgac aattgctgag caggccagtc tgcaacaaaa ggcgttattt
     5581 gatcaggtgc tgccagctga acgctataac aatgcgctgg cacagagttg ctatctggta
     5641 actgcgccag agttaggtaa aggtgagcat cgggtttaca tcgccaaaca ggatgacaaa
     5701 ccggtagctg ccgttctgga agcaaccgcg ccagatggct attccggtgc gattcagctg
     5761 ctggtgggag ccgattttaa cggcacggta cttggcacgc gcgtgactga gcaccacgaa
     5821 acgccagggc ttggcgataa aatcgaactg cgcctttctg actggatcac ccattttgcg
     5881 ggtaaaaaaa tcagtggtgc ggatgatgcg aactgggcgg tgaagaaaga tggtggtgat
     5941 ttcgaccagt tcactggcgc gacgattact ccccgcgcgg tggttaatgc ggtaaaacgc
     6001 gccggattgt acgctcagac gttaccggca caactttctc aacttcctgc ctgtggagaa
     6061 taaaacgtga gcgaaattaa agacgttatt gttcaggggt tgtggaaaaa caactctgcg
     6121 ctggtccagc tgctcggcct ttgtcctctg ttggcggtca cgtccactgc cactaacgct
     6181 ctgggtttag gacttgcgac tacgctggta ctgacgctaa ccaacctgac catttcgacg
     6241 ctgcgtcact ggacgcccgc cgagatccgc attcccattt acgtgatgat catcgcctcg
     6301 gtggtcagcg ctgtacagat gctgatcaac gcctacgcct ttggcctgta tcaatcatta
     6361 gggattttta ttccgctgat tgtcactaac tgtatcgttg tgggccgcgc tgaagccttc
     6421 gccgccaaaa aaggtccggc gctttcggca ctggacggct tttcaattgg tatgggcgca
     6481 acctgcgcca tgttcgtgct gggttcacta cgcgaaatta tcggcaatgg cacgttgttt
     6541 gacggtgcag atgcgctgtt aggtagctgg gcaaaagtat tacgcgtgga gattttccac
     6601 accgactccc ctttcctgct ggcgatgctg ccaccaggtg catttattgg cctgggactg
     6661 atgctggcag gaaaatacct gattgatgaa aaaatgaaaa agcgccgtac tgaagcagct
     6721 gccgaacgtg cattgccaaa cggtgaaaca gggaatgtct gatgaacaaa gcaaaacgcc
     6781 tggagatcct cactcgcctg cgtgagaaca atcctcatcc caccaccgag cttaatttca
     6841 gttcgccttt tgaattgctg attgccgtac tgctttccgc tcaggcgacc gatgtcagtg
     6901 ttaataaggc gacggcgaaa ctctacccgg tggctaatac gcctgcagcg atgcttgaac
     6961 tgggcgttga aggggtgaaa acctatatca aaacgattgg cctttataac agcaaagcag
     7021 aaaatatcat caaaacctgc cgtatattac tggaacaaca taatggcgag gttccggaag
     7081 atcgtgctgc gcttgaagcc ctgcctggtg taggtcgtaa aacagccaac gtcgtattaa
     7141 acactgcata cggctggccg actattgctg tcgacacgca cattttccgc gtttgcaatc
     7201 gtactcaatt tgcgccgggg aaaaacgtcg aacaggtaga agaaaagcta ctgaaagtgg
     7261 ttccggcaga gtttaaagtc gactgccacc attggttgat cctgcacggg cgttatacct
     7321 gcattgcccg caagcctcgc tgtggctctt gtattattga agatctttgt gaatacaaag
     7381 agaaagttga catctaaaga tcaggggtaa caccgattac cccattcata acctttcttt
     7441 atcctctttt aaaacatctt tttaacgtca atgatgccat tgcttagcgt tat
//
LOCUS       DACADA010000196         7492 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.198, whole genome shotgun sequence.
ACCESSION   DACADA010000196 DACADA010000000
VERSION     DACADA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.198"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..399
                     /locus_tag="ISB17_004243"
     CDS             <1..399
                     /locus_tag="ISB17_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982114.1"
                     /translation="YRDFVNSPSVLNIIATIIGYNECEGWLKSLKAHIASNIQFTQQY
                     LNENIPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGHVYGTEGEGY
                     LRLNLACSREKLYMGLTRLVSVIKNINQGE"
     gene            402..998
                     /locus_tag="ISB17_004244"
     CDS             402..998
                     /locus_tag="ISB17_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP1982115.1"
                     /translation="MNNTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            1070..2017
                     /locus_tag="ISB17_004245"
     CDS             1070..2017
                     /locus_tag="ISB17_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HAP1982116.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKILA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHAIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGRKIKNVL"
     gene            complement(2317..3693)
                     /locus_tag="ISB17_004246"
     CDS             complement(2317..3693)
                     /locus_tag="ISB17_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein VasL"
                     /protein_id="HAP1982117.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNVQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3693..4121)
                     /gene="tssE"
                     /locus_tag="ISB17_004247"
     CDS             complement(3693..4121)
                     /gene="tssE"
                     /locus_tag="ISB17_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAP1982118.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(4125..4661)
                     /gene="tssJ"
                     /locus_tag="ISB17_004248"
     CDS             complement(4125..4661)
                     /gene="tssJ"
                     /locus_tag="ISB17_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAP1982119.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4642..5721)
                     /gene="tssG"
                     /locus_tag="ISB17_004249"
     CDS             complement(4642..5721)
                     /gene="tssG"
                     /locus_tag="ISB17_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAP1982120.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5685..7445)
                     /gene="tssF"
                     /locus_tag="ISB17_004250"
     CDS             complement(5685..7445)
                     /gene="tssF"
                     /locus_tag="ISB17_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAP1982121.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1765 a         1923 c         1964 g         1840 t
ORIGIN      
        1 tatcgcgatt ttgtaaactc cccatcagta ctgaatataa ttgcaactat tattggctat
       61 aacgaatgtg agggatggtt aaagtcatta aaggcacata ttgcctctaa cattcaattc
      121 acccagcagt atctgaatga aaatattcca gaattgatcg taaggccagc tgatggttgc
      181 tatttttcct ggatagattg ttctgctatt ggttatcctt ttgatgaatt ctacagtcga
      241 ttaattcatg aaggcaaagt gggcattatg gcggggcatg tctatggaac ggagggggag
      301 ggctaccttc gtctgaacct ggcctgcagt cgagaaaaat tatatatggg gcttacgcgt
      361 cttgtgagtg taattaaaaa tattaatcag ggagaataaa aatgaataac acggatctta
      421 tccatctcat taaacatttt atgcgtaacg aacataaagc agttgaagaa gttattgatt
      481 cccctctttc ggaggtcgca aatttaatta aagtattaca atcttgtcag ggaaaagttg
      541 tttttattgg tgttggtaag tccggtatta ttgccaggaa actcgccgca acatttgcca
      601 gcaccggaac tccctcgttt tttgttcacg gtacggaagc tgtacacggc gaccttggaa
      661 tggtggcgaa agacgatgtt gttattctta tttcaaacag tggtgagacg gcggaaatcc
      721 tggcgacact gcctagtctg aaaaaaatgg gtaattattt gatctctttt acccgcagtc
      781 accactcatc gcttgcgata agttgtgatt tatctgttga aatccccgtc aaaagtgagg
      841 cggataattt aggtttagca ccgtcatgct cgtcgactgt tgttctggtt gttggtgatg
      901 cggtggcgct tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc
      961 atccaggagg tgcactcggc attaaagcaa attcataatt tcaaaatata aatacagtaa
     1021 ccggtcacgc catcttttgg ggtggcgttc tttgaagtga gtgtccgcta tgaaaaatgt
     1081 tctggtcacc gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga
     1141 gaataatttt aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac
     1201 tgcattacga gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac
     1261 gctatcattg ctgtctggtg tgaaaataat atcgagattc gggacgggga tagacaatat
     1321 tgatcttcgt gcggcacagc aatccggtat tgttgtcaat aatgcggttg ggattaattc
     1381 aaatgcggtt gcggaattta tcattggact tatcttcgcg agcatgagaa atatccctgg
     1441 cagctatcat gcgatgcaaa atggctactg gggtgagtcg catggctgtg aattacaagg
     1501 gaaacggatt ggcctggttg gctacggtaa tataggtaaa attctggcga aaaggttatc
     1561 cggttttgat gttgagcttt tagcttttga caaacaaccc gactatcagg ttgctgacaa
     1621 agctggagtt cagtttgtat caattgaaga tatctttatg cagtcgcatg ccatcattgt
     1681 tcttttgcct ttttcttctg agctggagaa cttcattagt cataaatatt tgtcgatgat
     1741 gcgcaatggt gctctgatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt
     1801 acttcaggtg attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc
     1861 gttggcgcaa tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc
     1921 ggccgccacc gttgagtcat accagcaaac cgggatacac gttgcccagt cgataattga
     1981 ttacttcgca ggaaggaaga taaaaaacgt gttgtgagtt cagcgaacaa agattttaaa
     2041 tacaaaaaaa ccgcaatttg tagaacacat tgcggtttgt cagcattcta ataaattggt
     2101 tgcgggggcc acagtcaaga ggttaatcca atcttttgaa acttaaggta ttcactgttc
     2161 ccgaacccaa gctatctacc gaaatataca ctcattatct gatgtgaatc aacgagtaga
     2221 tcgaatggcc tgttcctctc ttttttgtac acaaagactc cttttaaaaa atagcaaggt
     2281 cacatatccc ttattggtac ataaatcccc cgatgtttac tgactttgtt ccaccttcgt
     2341 atccagtaaa atgacatagc gattcagcaa ctgatttaac cgggtatcaa tctgcgtcaa
     2401 cagcgtcggc gaaatctctc cggtctgttt ttgctcttcc agttgccgaa gcagttcttc
     2461 cagcggcggc tcctttaacg actgccggat gccgtacacc gccgttttta gttccgaacc
     2521 ggtcagatat ctccccttac gttcatccag tgcatccagt tgtgccgaca ggcgctgtag
     2581 ctgactctgc gcctgtgcgt agccattcag tgcctctgcg gacagggcgc tcagttcccg
     2641 ctgtttacgc cactgcacgt tcagcgccct gacctgagga ttatccggcc acagggcatc
     2701 aagctggcgt agctggcgat atccctgctc cagcggccag cgggcaggta atttgtttaa
     2761 ctgctccagt tgcatctgac ttgccgcaat tgttctgtcc tgagccagca gcgccttatc
     2821 ctttacatcc agctttgcca gttcgccgct ctccagcgac tgcggcaacg gcatgacaga
     2881 cagttgtatt cgctggatta gcgcgtccgg ctgattaagc tgcgaccagc cccataatcc
     2941 tgttccgccg agaccggcca tcacaaccat tccggccata aatatcggcc atgtccgctg
     3001 tcgggagatt tcaggggatg acacgggtgg actggcatcc ttatcgtttt gctgagcggg
     3061 tctcgttgca aaaaccggct gtggactttc ttctgacttt ttcggttgat tcatttcccg
     3121 gcggggtaat tcagggggag tgatagtggt ttccgctccc tgaggcgcca gccgttccag
     3181 acgcaatgcg gtattgcgaa actgcccggc cagcacatcc agtttcgtca tatgccacag
     3241 ttcacactgt tgcagcgtgg tttcgatggc ggaaagatgc tgtacacaac ggtagatctg
     3301 cggcagatcc tgatactgaa tatccagcgt tctcagtgcc tgctgcattt tttctgtgac
     3361 ccagctgagc agtgatattc tggaatgtac ctgtaccggc cagaagtcag cccagcgctg
     3421 gagcagtata tccagtgagc cgagtccgtc tgccatccct gccagcccct gccgtctggt
     3481 tatcgccagt acgtaacaga ccagcgtctg tagttccacg ccattttgcc ggaacagcgc
     3541 gaggcagagt ttttccacct gacgccagtc cacatccggt cgggccgggt gagtcagttt
     3601 actcatttca gcctgcagtt caccggattc cttataagta cagggatcgc cgcccatgac
     3661 gaactggctg ataaaattcg cgttacttgc catcagtacg cccgctcctg ttgtttgaga
     3721 tgacgcagaa caattcgtcc ttccggagaa aatacgccat caaagcgtat ccagccggta
     3781 tcgggaagct gcgcgtggat caggtaatta agatgccccg gacggggctg ggggagtaac
     3841 tccacctgta tctgactcag gcgtggttca taacgcagca gcgtttcttc aatctgacgc
     3901 atcaggccat gaatacccgc agccatcccc tgatggatca gactgagatc cggcagaccg
     3961 taatccggca aatgcgccag cgtgcctgcc cggcagttga taatgcgttg catattgtcc
     4021 atcacggaaa ggataagctg atcccgctct ctgacctgtt ccagcggtaa ctcgccgctg
     4081 aaactgaacg tcagcatttc atataaggaa ggtgtcgaca tcggttattt atccttgaga
     4141 ggcagcagtc gcaggtcgct tctcatcagt tcaatcgagc gcggcttgtc ggcctccagt
     4201 tcgtcccttt tgatcaccag acgccagctg tcgctttttt catcagggtg ataaaactgc
     4261 ccaataatgg cgacaaactg cgtctcttta tccagcggca cattcaggga tgccgtgcct
     4321 tccggtttta cccagacggt gtgttttgcc agtacgttct tctccagcgt cttctcttcc
     4381 tgcataaaca gggtgtcata atccgccttg tcaaaatctt cgcgggtttt cagttgccag
     4441 acatgcacca tcgtggaaag cggcgtatct tccgcatccg tattcagggc acttcgggag
     4501 aagaaatcga gatgcagtat tttgatttgc ttgtagaaaa cggctgaggc cacgcttttc
     4561 gattcatcca ccactttctg aatcagcccg cagccgctca gaacaaccgt cagcatcgcc
     4621 atacagagca gcgccggacg tttaaaacga ataggaaaca tgagtcatac tctccctgtt
     4681 acgaatggaa aaagcgggta ttaatccctg ataacgcccc agactgaccg tcactgttcc
     4741 ggtcgcgggc gcggcaaagg aggcgcgaag gatgccggta cggctaatct gtacacgctg
     4801 ttttcccagc cgggcagcag gcaacaggct gacgggcagc gtcagttgca gtcgggcctg
     4861 acagcgccag cccagataaa cgcgcagcag caccagcaaa tcgctgtgca gttgcccgcc
     4921 gggcagccag ccacgtgctt ctgcggcatc ttcggtatac agtttcagca gcaactggct
     4981 gttgacgtcg gtgccggtac gccccagcag cgttcgcgtt ttcaggctga tgggtgaatt
     5041 ttttgacaga cccgcagggg ccgggagaat tacaggctgc ggatcatgag gcaccaccgt
     5101 cgctttcgtc agcggggcaa gcagtcccac cagtgccgta acgccttccg ccgtgcgcgt
     5161 cggcaggcgc atcacgctga gcagggccag aaaacgggat accggcgtgg cgatatggtt
     5221 ctcgctgccg ggaataccca gcccgatgag tcccagcagg caacgggaaa tgtcatccac
     5281 tgcacctgct tcaaacgacg ccgggtagtt atatttgcgc cagatgcggt aatactgggt
     5341 gataaagcga tggttaaaaa tatcgagaaa cgccatcacc gcgtcatgtc cgtcatgacg
     5401 ctgggtaatg tagtccagat aggcggttgg cagtggcgaa tccacgccgt acaggccgag
     5461 aaaagtggtg cgcactgtgg gtggcgcgtc gggattgtcc acgtcacgtt caacgttctt
     5521 taactcgctg accggaaagc ccatccccgg atgaggacga aagcgcaccg catcggcggc
     5581 ggggctgtcg gtactgccca gcggcgcgtt ctgctggcgc tgttccagca actggcaaaa
     5641 acggtaaaaa ttgatccgcg caatatccgg ggccagccgg tcggtcagcc cggtacgcgc
     5701 tggctgtgat gctctgtcca ttgcaggcac tttcctgtgg gttgcagaat taaggttagt
     5761 tgagtgaata agtggatatc ggcatacagt gcgaagaagc ggtgcagcag ttcaccaaac
     5821 agcgtgatat caccttctcc ggcaaagccg ttactgtcca gcgtcacctg aatatccacg
     5881 ccacgcagca gaaagcctct ttcaaaacgc tgtatcaggc tgtgctgaac gtcgacaatc
     5941 gccgccagac gacggcggtt catctcgctt tcggtccagt cgtacagcgc cagcgtgccg
     6001 cgcagaattt cggcgttatc catcatcgat aaaaagttag aaccaaggtg gctgagcacc
     6061 cgccagtgga agcggtcacg cgccggggga tagcagggct gcgtcggggc gcacaggttg
     6121 cgcacccgca ggacgttctg tgaggcatga actggcgtat cgagcagcgt gctctgtaaa
     6181 gctttacggg gaagctgccc gttagtaccg gtcaggctta aggacagggt ttcatcctcc
     6241 agcatccggt cagtatcaaa cgcatcgccg cccagaatca gccaggtgtc atgcaggccg
     6301 gaagggccgc gctttacccg cgtgtggtaa tagcgttccg gcgcatcgtg gcgcagcatc
     6361 ccgccccggt gacgaaagct ggagaatggc acatacgcct gactgccggt atggcgcgaa
     6421 gaaataatgt tgtcgacgga ataaatctcg gtatgcccgt cctgaatgcg catcgggcgc
     6481 agcagaaatt cgttttccag tggcgaaagg tgtaacggat cggcttccag cggaaacagg
     6541 ttgatgaccg gcacgcagtg cagacggata ttatcgctgt caaacggcag gtcgtgaggc
     6601 cagttctcgt tcagcaacac gtcaatctca aagccggtta tcccctccgg ccatgcaacc
     6661 tgctccagcc cgttcagcgc cacgaacata aacttctcgc ggaaggtgaa atactccagc
     6721 agcaactggt agccgctgaa cgccgattcg cctttcggcc acagtcggtc atcgtcatca
     6781 aaacccatcg gggaaaagtg cgcgtccagc acacggcgtc cctgccctgg cagacgcagc
     6841 cagaactgct gcgtaccaag cgttaaagcc cggtgcagcg cacaggccac cgggctgtcg
     6901 gcactgaggt acagcggcag gcgggataaa tcaatctgtg accagtcacc aaccagcggg
     6961 ccgcattcaa agcgcagacg gataaccgag cgtccgtccg gttcatattg caatgacacg
     7021 tccggcaacg ccagcggatg cagcgtgata tcccgcgtgg cgctgtagac gcagcgggtt
     7081 ctgcgttcgc cgatgggacg ggatagcacc tgaaactctt tcagcgtatc actttgcttc
     7141 atttgttggt gatccgtggt cagttcgacc acggaaagcg acggaatagt gcgcaggtag
     7201 tgcggccaca gcaggctgac cagcccttca gtcagttccg gcagatcgtc gtccagcttt
     7261 tcgcgcaggc ggcccattaa aaaagcgaag ccctcgaaca ggcgctccac atagggatcg
     7321 cgtgcgccgg gtttatcaag attaagcatc gcagcgcggt cggggtaagc ctgcgcaaat
     7381 tctttccccg cttcacgcag gtagcgcatt tcggcctcaa agtagcgttg ggtcaggtcg
     7441 tccatcggag gatttatctt tccttttatt gaaattaata ccaacgagta gt
//
LOCUS       DACADA010000197         7454 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.199, whole genome shotgun sequence.
ACCESSION   DACADA010000197 DACADA010000000
VERSION     DACADA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.199"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            167..4414
                     /gene="fdeC"
                     /locus_tag="ISB17_004251"
     CDS             167..4414
                     /gene="fdeC"
                     /locus_tag="ISB17_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HAP1982122.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWIAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4539..5396)
                     /locus_tag="ISB17_004252"
     CDS             complement(4539..5396)
                     /locus_tag="ISB17_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1982123.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5644..6513
                     /locus_tag="ISB17_004253"
     CDS             5644..6513
                     /locus_tag="ISB17_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1982124.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6673..7266)
                     /gene="rclC"
                     /locus_tag="ISB17_004254"
     CDS             complement(6673..7266)
                     /gene="rclC"
                     /locus_tag="ISB17_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAP1982125.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7278..>7454)
                     /locus_tag="ISB17_004255"
     CDS             complement(7278..>7454)
                     /locus_tag="ISB17_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1982126.1"
                     /translation="NADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKVTSM
                     RIDNNSTATAVLYK"
BASE COUNT         2042 a         1658 c         1915 g         1839 t
ORIGIN      
        1 tggctacaga cactggatat attaggtggc ggtgtacttt cttacataaa ggtatttcct
       61 tttctgtgga aaaggaaacc gggaaatccc cggtttttct gacaagcaga cgccattatt
      121 tgtgtctgcc tatgttcgtt aattcgttca tcaggaaatt atctcaatgt cacgttataa
      181 aacagacaat aaacagccac gatttcgtta ttcagttctg gcccgctgcg tggcgtgggc
      241 aaatatctct gttcaggttc tttttccact cgcagtcacc tttacgccag taatggcagc
      301 acgtgcgcag catgcggttc agccacggtt gagcatggaa aatactacgg taactgctaa
      361 taataacgtg gagaaaaatg tcgcgtcgct tgccgctaat gccgggacat ttttaagcag
      421 tcagccagat agcgatgcga cacgtaactt tattaccgga atggccaccg ctaaagctaa
      481 ccaggaaatt caggagtggc tcgggaaata cggtactgcg cgcgtcaaac tgaatgtcga
      541 taaaaatttc tcgctgaagg actcttcgct ggaaatgctt tatccgattt atgatacacc
      601 gacaaatatg ttgttcactc agggggcaat acatcgtacc gacgatcgta ctcagtcaaa
      661 tattggtttt ggctggcgtc atttttcaga aaatgactgg atggcggggg tgaatacttt
      721 tatcgatcat gatttatccc gtagtcatac ccgcattggt gttggtgcgg aatactggcg
      781 tgattatttg aaactgagcg ccaatggtta tatccgggct tctggctgga aaaaatcgcc
      841 ggatgttgag gattatcagg aacgcccggc gaatggctgg gatattcgtg ctgagggcta
      901 tttacctgcc tggccgcagc ttggcgcaag cctcatgtat gaacagtatt atggcgatga
      961 agtcgggctg tttggtaaag ataaacgcca gaaagatcca catgcgatta ccgctgaagt
     1021 gaattacacg ccagtgcctc ttctgaccct gagtgccggg cataagcagg gcaagagtgg
     1081 tgagaatgac actcgctttg gcctggaagt taattatcgg attggcgaac ctctggaaaa
     1141 acaactcgat acagacagca ttcgcgagcg tcgaatgctg gcaggcagcc gctatgacct
     1201 ggttgagcgt aataacaata tcgttcttga gtatcgcaaa tctgaagtga tccgtattgc
     1261 tctgcctgag cgtattgaag gcaagggcgg ccagacggtt tccctggggc ttgtggtcag
     1321 caaagcaact cacggtctga aaaatgtgca atgggaagcg ccgtctttgc tggccgcagg
     1381 cggaaaaatt acggggcagg gcaatcagtg gcaagtgacg ctcccggctt atcaggcagg
     1441 caaagacaat tattatgcga tttcagcgat tgcctacgat aacaaaggca atgcctcgaa
     1501 acgtgtgcag acagaagtgg ttattagcgg agctggtatg agcgccgatc gtacggcgtt
     1561 aacgcttgac ggtcagagcc gtattcaaat gcttgctaac ggtaatgagc aaaagccgct
     1621 ggtgctgtct ctgcgcgacg ccgagggtca gccagtcacg ggcatgaaag atcagatcaa
     1681 gactgaacta accttcaaac cggctggaaa tattgtgact cgtaccctga aggccactaa
     1741 atcacaggca aagccaacac tgggtgagtt caccgaaact gaatcagggg tgtatcagtc
     1801 tgtctttact accggaacgc agtcaggtga ggcaacgatt actgttagcg ttgatgacat
     1861 gagcaaaact gtcactgcag aactgcgggc cacgatgatg gatgtggcaa actccaccct
     1921 gagcgctaac gagccgtcag gtgatgtggt tgctgatggt cagcaagcct acacgctgac
     1981 actgacagcg gtggactccg agggtaatcc ggtgacggga gaagccagcc gcctgcgact
     2041 tgttccgcaa gacactaatg gtgtaaccgt tggtgccatt tcggaaataa aaccaggggt
     2101 ttacagcgcc acggtttctt cgacccgtgc cgggaacgtt gttgtgcgtg ccttcagcga
     2161 gcagtatcag ctgggcacgt tacaacaaac gctgaagttt gttgccgggc cgcttgatgc
     2221 agcacattcg tccatcaccc tgaatcctga taaaccggtg gttggcggta cagttacggc
     2281 aatctggacg gcaaaagatg ctaatgacaa ccctgtaact ggcctcaatc cggatgcacc
     2341 gtcattatcg ggcgcagctg ctgctggttc tacggcatca ggctggacgg ataatggcga
     2401 cgggacctgg attgcgcaga tttctctcgg cactacggcg ggtgaattag acgttatgcc
     2461 gaagctcaat gggcaggacg cggcagcgaa tgcggcaaaa gtaaccgtgg tggctgatgc
     2521 attatcttca aaccagtcga aagtctctgt cgcagaagat cacgtaaaag ccggtgaaag
     2581 cacaaccgta acgctggtgg cgaaagatgc gcatggcaac gctatcagtg gtctttcgtt
     2641 gtcggcaagt ttgacgggga ccgcctctga aggggcgacc gtttccagtt ggaccgaaaa
     2701 aggtgacggt tcctatgttg ctacgttaac tacaggcgga aagacgggcg agcttcgtgt
     2761 catgccgctc ttcaacggcc agcctgcagc caccgaagcc gcgcagctga ctgttattgc
     2821 cggagagatg tcatcagcga actctacgct tgttgcggac aataaaactc caacggttaa
     2881 aacgacgacg gaactcacct tcaccgtgaa ggatgcgtac gggaatccgg tcaccgggct
     2941 gaagccagat gcaccagtgt ttagtggtgc cgccagcacg gggagtgagc gtccttcagc
     3001 aggaaactgg acagagaaag gtaatggggt ctacgtgtcg accttaacgc tgggatctgc
     3061 cgcgggtcag ttgtctgtga tgccgcgagt gaacggccaa aatgccgttg ctcagccact
     3121 ggtgctgaat gttgcaggtg acgcatctaa ggctgagatt cgtgatatga cagtgaaggt
     3181 taataaccaa ctggctaatg gacagtctgc taaccagata accctgaccg tcgtggacag
     3241 ctatggtaac ccgttgcagg ggcaagaagt tacgctgact ttaccgcagg gtgtgaccag
     3301 caagacgggg aatacagtaa caaccaatgc ggcagggaaa gtggacattg agcttatgtc
     3361 aacggttgcg ggagaacaca atatttccgc ttcggtgaat ggtgctcaga agacggtcac
     3421 ggtgaaattc aacgcggatg ccagcaccgg tcaggcaaac ctgcaggtag acaccgccgt
     3481 tcaaaaagtg gcaaacggca aagatgcctt tacgctgacg gcgaacgttg aggataaaaa
     3541 tggtaaccct gttccaggga gcctggtgac ctttaatctg ccccggggtg tcaagccgct
     3601 tacaggcgat aatgtgtggg tgaaagccaa cgatgagggg aaagcggagt tgcaggtggt
     3661 ttccgtgact gccggaacct atgagatcac ggcgtcagca ggaaatgacc agccttcgaa
     3721 tgcgcagtct gtaacgtttg tggccgataa gactacggcg accatctcca gtattgaggt
     3781 gattggcaac cgtgcagtgg cggacggcaa aaccaaacag acgtataaag ttacggtgac
     3841 tgatgccaat aacaacctgc tgaaagatag cgaagtgacg ctgactgcca gcccggaaaa
     3901 tttagttctg actcccaatg ggacggcgac aacgaatgag caagggcagg ctattttcac
     3961 cgccacgacc actgtcgcag cgacatatac actcacggcg aaagtggaac aggccgacgg
     4021 tcaggaatcg acgaaaactg ccgaatctaa attcgtcgcg gatgataaaa acgcggagct
     4081 cgccgcaaca tctgatgtgc attctctggt ggcggatggg gtaacgacag caacactgac
     4141 ggtaaccctg ttctcggcaa acaacccggt aggaggaacc atgtgggtcg acattgaggc
     4201 tccggaaggg gtgacagagg cggattatca gttcctgccg tcgaaaaatg accatttcgc
     4261 gagcgggaaa atcacgcgta catttagtac caacaagcca ggtacataca cattcacatt
     4321 caactctttg acatatggag ggtatgaaat gaaacccgtg actgtgacaa ttaacgccgt
     4381 tcctgcagat actgaaggtg ctgaggagaa ataaaaacga cgatgtaata aataataatg
     4441 gcgttgacat gcgcagcgcc attatttact gaatacctac cctggcaccg attagacggt
     4501 ggcagggtag ggtaaatctt atgtgatgga gttacaggct aaaccgggcg aacggggata
     4561 aaaatttcgc aaacaatgtt attgctatca atttcgacga agttttcatt gtaataaaat
     4621 cgctcaatat cactgccgtt cactctggct aaccccctgg caggtaattc catcagatag
     4681 aggtttcgcg cccattcagg gtagtgctcc cactcacctt catagcggaa agaggcatac
     4741 agtcctcggt taattgtcca ctctttcggc atctctgaat cggtttgttt gcataccgta
     4801 ccggcaaaag tttcaactgt gagcaaatcg accgattttg ttgatggttc gaacgtggag
     4861 acgaaataga tatccgtctt aaaattcttc atacatctga ctaaatgatg acgtcgcagt
     4921 ttgacttctt catccggtaa tttattcgac acttcaatat catatttaaa tgaattgcca
     4981 aaaataggtg tctcaggaaa gttgactaat ctacactccg tcttttgctg ttgacgtatt
     5041 aaaaaagaag ggaagatatt tgcgagatcc caataatcac ggtggcggta ttcacgggga
     5101 gagcagccga ataacttttt aaattcgcgg ctgaatgact gctgtgaatc aaaatgcaaa
     5161 gagagtgcaa tatcaagcat agatttcgcg gagagccgga caagaatggc ggcacgacaa
     5221 agccttcgtt tgcgaatgta ttctcccaaa ggcacatgca tgaaatttcg gaacagaagc
     5281 tggatgttgc gtctgctgta gccagatttc tgtgcgatat cttcgattga aatagggtgc
     5341 tcaagattgc actcaatcca ctccaggagc tgctgtagaa tcttctgcct gatcatcaca
     5401 tactccgtta attctttgtc atatcgcaca ttttcgttat gtcctgggcc ctaagtgtat
     5461 caaaaacagc aagtcccaag gcaaaagcac tgatagcgtc tgcttcattg ccccaaaaat
     5521 agagaggttt cagtcctaca ttattaatga attttttgca taagtgatat caaaatccac
     5581 atactaattt gaggttacgt tttaacgtag actcattgtt catacctaat ggagggactg
     5641 acagtggaat tttccgtatt aagtaacaat ctgaaaatgc cgatgatggg atttggtgtt
     5701 tttcaggtta ccgataaaga cgtgtgcaaa cagtcagtgt tgaatgctat ccgcacgggt
     5761 tatcgactca tcgacactgc agcggtatac ggtaatgaag atgccgtcgg tgaagccgtt
     5821 cgtgaagcca tatctgaagg tttatgtacc cgagaagagt tatttataac gtctaaattg
     5881 tgggtgcagg atatgttgaa tcaggatatt gcagcagcag gtattgaagc atcattaaaa
     5941 aaatcggggc tagaatactt cgatctctat ttattgcacc aggccatgcg cgattatttt
     6001 agtgcgtggc gtgcactcga agatgcctat gaagagggca aattaaaagc gattggggtt
     6061 tccaatttct accctcatgt tctggcgaac ttttgtgaaa cggtaagagt taaaccgatg
     6121 gtcaaccagg tcgagttgca tccctatttt gcccaaccag aggcgctggc aaccatgaag
     6181 tattataacg tgcagcctga agcatgggct ccgttaggtg gtggacgaca taaacccttt
     6241 gaaaataatc tgcttcagag tattgcagat gcccatcaaa aatcgatttc tcaggtcatt
     6301 ctgcgttgga atattcaacg gggagtggtc gttattccga aatcgacaca tcaacagcgt
     6361 atcgaagaaa attttgctat ctgggatttc tcactgacag agaaagaaat ggcgcaaatt
     6421 agttcgctag atttgggtta tgttggggaa tcggtaaaac attttaatcc tgagtttgtt
     6481 cgtggttgtc ttgctgtaaa aatacatgat tgatattaat cacttatttt acctgagacg
     6541 acaaaaattt tttaacaggg gagtgatatt gatcgtcact ctgtcatatc tccggtaata
     6601 tggcgtcagg cttcatcaac agaaaggggg cctgacgcct gaaaaagtga acaacagaca
     6661 gtgttcggat tatcaatatt cagtttttaa cgttgaactg gattcattac tgcgttgttt
     6721 aagaatatcc cgtgccgaat ctgccattat catgacagca cctgccagca tcagagtatc
     6781 tttcaatacc agacgaccag caccggataa ataagggaaa ccatgatgag catcacccaa
     6841 tgcgggtacc catgcctccg gagtggtgat taaaaatgag agtgttacca gcggcgtggt
     6901 aaatgccatc agcccaccca ataaacctaa ccagcgattg acaggattag ccaaaaccag
     6961 cagagcaata atcacctcca cgacgccaag accgttggaa aaaccatagg tattattagc
     7021 cgtttgccat gctcttgctt ctggtttgta ttcgccttcg tgagtcagat actgtttata
     7081 gtcttccggg tgttcataaa agaacgacat tagtggactg tttgcgacga atggtgtaat
     7141 gctgtctgcc tcgtaaggga caaactttaa taacccaatc cacataaaaa caatcgcaat
     7201 gctcagacga atcaatgtca ggccaatttt atctccccga cttaacaggt gcaggtatct
     7261 ttccattgta aacatcctta tttatacaat actgctgttg cggttgaatt attatcaatt
     7321 cgcatcgatg taactttcca ggatgaagca ttatattttc tggccatatc agcaatggca
     7381 ttctcaagaa ctgtcggtga tgctgatact gatgagacgc tgatatgttt cagaattatt
     7441 ttatcatctg catt
//
LOCUS       DACADA010000198         7446 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.200, whole genome shotgun sequence.
ACCESSION   DACADA010000198 DACADA010000000
VERSION     DACADA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.200"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            139..378
                     /gene="chaB"
                     /locus_tag="ISB17_004256"
     CDS             139..378
                     /gene="chaB"
                     /locus_tag="ISB17_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAP1982127.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            527..1222
                     /locus_tag="ISB17_004257"
     CDS             527..1222
                     /locus_tag="ISB17_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP1982128.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1266..1619)
                     /gene="ychN"
                     /locus_tag="ISB17_004258"
     CDS             complement(1266..1619)
                     /gene="ychN"
                     /locus_tag="ISB17_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAP1982129.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1804..3198
                     /gene="ychO"
                     /locus_tag="ISB17_004259"
     CDS             1804..3198
                     /gene="ychO"
                     /locus_tag="ISB17_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAP1982130.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3199..3849)
                     /gene="narL"
                     /locus_tag="ISB17_004260"
     CDS             complement(3199..3849)
                     /gene="narL"
                     /locus_tag="ISB17_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAP1982131.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3842..5638)
                     /gene="narX"
                     /locus_tag="ISB17_004261"
     CDS             complement(3842..5638)
                     /gene="narX"
                     /locus_tag="ISB17_004261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAP1982132.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5977..7368
                     /gene="narK"
                     /locus_tag="ISB17_004262"
     CDS             5977..7368
                     /gene="narK"
                     /locus_tag="ISB17_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAP1982133.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1738 a         1820 c         1960 g         1928 t
ORIGIN      
        1 ataaaaatta gtaatacgtt tagacaatgt ttgttttagc acttcttaag aagagtctga
       61 catgaaaatt cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact
      121 ttgtaaaagg agtcaacgat gtcgtataaa acgaaaagcg atctgccgga aagcgtaaag
      181 cacgtgcttc cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat
      241 caatataaag ataaagaaga tcggcgtgat gacgccagtc gcgaagaaac agcgcataaa
      301 gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa
      361 aaatcgtcaa accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc
      421 cgtggattgc atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga
      481 caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga
      541 tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg
      601 gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg
      661 accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga
      721 gtcgtgcacc ggtacactgg ttggatggca tcgcgcgttt tgcctgcgtc tgaccgccgg
      781 gcgcgggact gcgcaccagc cgggacgaat gcttgcactg aaagagggcg gacgcaccac
      841 aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc tgctgtggaa
      901 gcgtgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg
      961 acgcacagta aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga
     1021 cactcgcgct caggtcatag cgccgttgat tgccgcggcg agcggtccgc tgggaaccaa
     1081 tgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg
     1141 gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt
     1201 gttacggccg ggattcgcct gagtaaactt ccctcatagt ggggcgtccg acgccccctc
     1261 aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag ttcaacaaga
     1321 gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag
     1381 gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg
     1441 ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg
     1501 aacagacgca gatccagatt gttctcctgc tctcgtaacg caatggccag ccgcaagctg
     1561 ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt
     1621 atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt
     1681 ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag
     1741 gtgccagtaa tcgttattct ttaaggctat ggtttttcat ttttaccgga agttaccgac
     1801 gttttgagcc gtttcgttcc tcgcattatt ccgttttatt tactcttgct ggcggcaggc
     1861 ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatcactt tgagaatagc
     1921 aatgatggtc tgccggattt aggcatggca cctgaacatc atgatgggga aaaacacttt
     1981 gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc
     2041 gagcaggcaa aagctttcgc attgggaaaa gtccgcgatg cgcttagtca acaggttaat
     2101 cagcacgtag agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt caaagtggat
     2161 aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca agataatgat
     2221 cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggct ggtgagcaat
     2281 gtgggcgttg ggcaacgctg ggcgcgcggc agctggctgg tgggttataa cactttttat
     2341 gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gcgctgaagc gtggggcgaa
     2401 tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga acagactgcc
     2461 acgcaggaac aacggatggc gcgcgggtac gacctgacag cccggatgcg catgccgttc
     2521 tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgtcgatttg
     2581 tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc
     2641 cctgtgccct tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa
     2701 aataacctcg ggctgaatct taactaccgc tttggtgtac cgctcaaaaa acaactttct
     2761 gcgggcgagg ttgtcgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga
     2821 aataatcttc cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca
     2881 ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt
     2941 tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc
     3001 gcacaagcca acagtgagga gggctggacg ctgatcatgc ctgactggca gaacggggaa
     3061 ggggcgagca atcactggcg attgtctgta gtggtggaag ataaccaggg gcagcgtgtc
     3121 tcctccaatg agatcacgct aacgcttgtc gaaccgttcg acgcattgtc aaacgacgaa
     3181 ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg cagcttccac
     3241 gcgagacttt agcttcattt tcttcagcat gtgcttgacg tgaactttta ctgtgctttc
     3301 ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag
     3361 aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc
     3421 gcgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc
     3481 tgcctgatgt aacgctttca gcagatcttc cggttccata tcttttaaca gatagccatc
     3541 cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac
     3601 aatgcgcccg gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc
     3661 gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca gttcaatacc
     3721 ctgttcgcca ttactcgctt cgccaaccac agtgatatct ggtgccatac tgataagctg
     3781 ttttacgcca gttcgcaaca tcgggtgatc gtcgatcagc aggatagtag ccggttcctg
     3841 attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca
     3901 ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg
     3961 cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc
     4021 cgcagccgtt atcctggacg gtcagtttga cctgattatc gttttgcgcc accgtcacca
     4081 cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca
     4141 acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca
     4201 ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc
     4261 caggctcggt gagctgcaaa cggaatgtgg tgagcaattc acgcaactgc acccaggatg
     4321 cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc
     4381 cctgcatctg taagcaactc acctgcatct tcatgcaaga gagagattgg gcaatagaat
     4441 catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt
     4501 cctgatggcg atccagcgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt
     4561 gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact
     4621 gcgtatgagt gtcagccagc cgccacttca gggttgtgcc gcgatcgcca acgggtaata
     4681 cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctgac tggcaggtaa
     4741 actcctgatg attctcttca tcatcagtgt catacacccg cagttcgata tcacgtagca
     4801 gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcgcggg
     4861 aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta tgctccagcc
     4921 cagcggtttt ctcctgaacc cgctgctcaa gtacggcata actttccgcc agttctgcag
     4981 acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc
     5041 gttgggtaaa atcgcgatgg ctgacggcac tcgccattgc cagcagttgc cgccacggtt
     5101 gtagcaatct cgcccgcaac cagataatag tgaataccag taaaagtgcc ataaataccg
     5161 ccattacccg atggaccagc accactgtct caatgcgcat ttccgtggtg cgatcaaaac
     5221 cagataccag ctgatcaagc ccggcaacaa actggctgac atccgctgac accgtttcgc
     5281 ggttttgtgc acgtatcagt gcagggatca gttcattacg ccagtaatct tgtaaaccct
     5341 gtaattgcgc cagttgcccg tctcgttctg ctgctcgagt caactcggcg ctaaatgctg
     5401 tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact gctgccaaca
     5461 gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa
     5521 cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca
     5581 acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt
     5641 aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct
     5701 ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa gagttactcc
     5761 ttatttgccg tgtggttagt cgctttacat cggtaagggt agggatttta cagtaccgtg
     5821 aaaaatctca taatttttat gaaatcactg tactcactat ggataatgat aaatatcaat
     5881 gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct acagacacta
     5941 aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc cgcccccgaa
     6001 agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt ctggcaacaa
     6061 cgcggtcaac gtattgccag ccgcaacctg tggatttccg ttccctgtct gttgctggcg
     6121 ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt cggttttaat
     6181 tttacgaccg atcagctatt tatgttgact gcgctgcctt cggtttctgg cgcgttatta
     6241 cgtgttccat actcctttat ggttcctatc tttggtggtc gtcgctggac ggcgttcagc
     6301 accggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg
     6361 ccttatagcg tcttcatcat catttctctg ctgtgcggct ttgctggcgc gaacttcgca
     6421 tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt
     6481 ctgaatggtg gtctgggcaa catgggcgtc agcgtcatgc agttggttgc tccgctggtg
     6541 gtatcactgt cgattttcgc agtatttggt agccagggcg tcaaacagcc ggatgggact
     6601 gagctgtatc tggcgaatgc gtcctggata tgggtgccgt tccttgccat cttcaccatt
     6661 gcggcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa ggagcagttg
     6721 ccggtactca aacggggtca tctgtggatt atgagccttc tgtatctggc aaccttcggc
     6781 tccttcatcg gcttctccgc gggctttgcg atgctgtcaa aaacgcagtt cccggatgtt
     6841 cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg ttctgcaggt
     6901 ggtgcattat ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt tatcctgatg
     6961 gcgattttca gcggcctgct gttcctgacc ttaccgactg acggacaggg cggaagcttc
     7021 atggcgttct tcgcagtctt ccttgcgctg ttcctgaccg ctgggctggg tagtggttcc
     7081 actttccaga tgatttccgt gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa
     7141 gggggttctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc ggcgctgggt
     7201 ttcatctctg cgattggcgc gattggtggc ttctttatcc cgaaagcgtt tggtagctcg
     7261 ctggcattaa cgggttcgcc agtcggcgca atgaaagtat ttttgatttt ctatatcgcc
     7321 tgcgtggtga ttacctgggc ggtatatggt cggcattcta aaaaataaat cgtaatattt
     7381 gattatcctt tgcgcggcat gatgtcgcgc tttttttatg tgtcatttag ttacaacata
     7441 ctaatg
//
LOCUS       DACADA010000199         7369 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.201, whole genome shotgun sequence.
ACCESSION   DACADA010000199 DACADA010000000
VERSION     DACADA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.201"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(181..771)
                     /gene="cobO"
                     /locus_tag="ISB17_004263"
     CDS             complement(181..771)
                     /gene="cobO"
                     /locus_tag="ISB17_004263"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP1982134.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(768..1526)
                     /locus_tag="ISB17_004264"
     CDS             complement(768..1526)
                     /locus_tag="ISB17_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAP1982135.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1746..2795
                     /gene="sohB"
                     /locus_tag="ISB17_004265"
     CDS             1746..2795
                     /gene="sohB"
                     /locus_tag="ISB17_004265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAP1982136.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2831..3082)
                     /locus_tag="ISB17_004266"
     CDS             complement(2831..3082)
                     /locus_tag="ISB17_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAP1982137.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3462..6059
                     /gene="topA"
                     /locus_tag="ISB17_004267"
     CDS             3462..6059
                     /gene="topA"
                     /locus_tag="ISB17_004267"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP1982138.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            6269..7243
                     /gene="cysB"
                     /locus_tag="ISB17_004268"
     CDS             6269..7243
                     /gene="cysB"
                     /locus_tag="ISB17_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAP1982139.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         1893 a         1731 c         1915 g         1830 t
ORIGIN      
        1 tgctaatgat ttgatcactg aattaatact gtttattgaa gacgtcaatg cggataatga
       61 tatggaatat cccgcacact tgaataaaat tcaagctctt cttaattttg ccgtggctaa
      121 ttaagccatt cagtatctaa aattagcacc tcaagaggcg aacttttgag gtgctttcac
      181 ttagtaatca atccctatct gcgcttttac accggcatca aacgcatgtt tgatggggcg
      241 cagctcactt accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat
      301 aatcaccgtc tgttgatgtg gacgttcatt taacgcctga atcacgtctt ccagtggcaa
      361 atagtcatac gctaccatat atgtcagttc atcgagcaaa accatatcca gtgaggaatc
      421 agccagcatc cgctttgcat gttgccagac ttcgcggcag gcggcggtat cggactctcg
      481 gttttgtgta tcccaggtaa agcccgttgc cattacctga aactcaacgc cgtgtggctc
      541 cagcagattt cgttcaccat taggccaggt gcctttaata aactgcacga cgcccacttt
      601 ttttccgtga ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc
      661 attgccggta aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc
      721 tactttttct ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt
      781 cctggtttac ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg
      841 cccatcagcc agaggtagag cggcatgata tcagcggggg ttttaagttt ctgtggatct
      901 tcggtcggga aggcactggc ccgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg
      961 acacgcaggc gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat
     1021 ttcgacgctg catatgcgcc ccagtttgca cgcccctgac gtccaacgct tgatgaggta
     1081 aagaccagtg aaccggcgtc ggatttgagt aataaaggaa gcagtgcctg ggtgagcata
     1141 aaggtggcat taacgttgac ctgcatgacg tcctgccaga cttgcggatc ttgttcgctc
     1201 attgggcaaa catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa
     1261 ttaacggcaa tgcgctgtgc cagttgttgg caatcttcgg acgtgcaggt tagcaaatcg
     1321 agaataaacc actgtggctg acgtccagtt tcttcgttta tgtggctggc tacctgacgt
     1381 aatttttctt cattacggcc caaaagaatc actgtcgcac catagcgtgc atacgtcatc
     1441 gctgcttcac gaccaattcc atcgctggct cccgtcacca gaataatgcg atcattgagt
     1501 aaatcttgtt ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc
     1561 cagtgtactt tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct
     1621 ggggcgataa caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc
     1681 gagacggcta aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg
     1741 gactcgtgga attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc
     1801 tagcgattgc ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc
     1861 gtggcgagtt acgagtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg
     1921 ccgcggcgct gatggacact catcagcaaa aacagtggca caaagcgcag aagaaaaagc
     1981 acaagcaaga agcgaaagcc gcaaaagcga aagctaaact gggcgaggtg gtaactgaca
     2041 gtaaaccccg cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact
     2101 ctctacgtga agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc
     2161 tccgtctgga aagcccaggt ggcatggtgc atggttacgg gttggcggct tcgcagttgc
     2221 agcgtctgcg tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg
     2281 gcggttacat gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg
     2341 gttccattgg ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata
     2401 ttgatatcga actgcacacc gccgggcagt ataagcgcac gctgacgttg ctgggtgaaa
     2461 ataccgaaga agggcgggag aaattccgcg aagagttgaa cgaaacgcat cagttattta
     2521 aagattttgt gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac
     2581 actggtacgg acaacaggca gtagagaaag gcctggttga tgaaatcaac accagtgatg
     2641 aagttattct tagcctgatg gaaggccggg aagtggtcaa tgtacgctat atgcagcgta
     2701 aacgactcat tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgttac
     2761 gctggtggca gcgtgggcaa aagccgttga tgtaaaagac aaacgcgagg ataagacctc
     2821 gcgttttgct ttaatcaacc aggtgatatt tttctgaaag cacatgggcc aggtgtttga
     2881 acatattaaa caccgcggtg cttttggcag ttggtaatcc ttgttcatct aaaaagtagt
     2941 cgccggtaaa taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga
     3001 gcgtatcctc atgctcacga atgattttgt tggcctcttt caacagcgtt tcgcggtcga
     3061 tgggttgagt ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg
     3121 catggcttgc agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga
     3181 ttttgcatgc taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc
     3241 aatctggcaa tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta
     3301 ggccaagacc tgttaactca gtcacctgaa ttttcgtgag cagagtcacg acaaggggtt
     3361 gatatccgca gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg
     3421 tgcgacgcat tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca
     3481 tcgttgagtc cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg
     3541 tgaaatccag cgtcggtcac atccgcgatt tgccgaccag tggctcaact gccaaaaaga
     3601 gtgccgactc tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc
     3661 tcgtcaaccg tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc
     3721 ctggtaaaga gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct
     3781 atctcgcaac cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga
     3841 ttggtggtga tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga
     3901 tccgccaggc atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg
     3961 cgcgtcgctt tatggaccgc gtggtggggt atatggtttc gccgcttcta tggaaaaaga
     4021 tcgctcgtgg tctgtctgcc ggtcgtgtgc agtcggtggc ggtccgcctg gtggtcgagc
     4081 gtgagcgtga aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga
     4141 ccacgccatc tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc
     4201 gtccggtcaa caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca
     4261 gcgtgctgga acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct
     4321 ctacgctgca acaagctgcc agcacccgtc ttggttttgg cgtgaaaaaa accatgatga
     4381 tggcgcagcg tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccaccaacc
     4441 tgagtcagga cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat
     4501 atctgccgga aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag
     4561 cgattcgtcc ttctgacgtc aatgtgatgg cggaatcgtt gaaggatatg gaagcagatg
     4621 cgcagaaact gtaccagtta atctggcgtc agttcgtagc ctgccagatg accccagcga
     4681 aatatgactc cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc
     4741 gtattttgcg ctttgatggc tggacgaaag tgatgcctgc actgcgtaaa ggcgatgaag
     4801 atcgtatctt accggcagtc gataaaggcg atgctctgac gctcgttgaa ctgacaccag
     4861 cccagcactt taccaagcct ccagcccgtt tcagtgaagc atcgctggtc aaagagctgg
     4921 aaaaacgcgg tatcggtcgt ccttctacct atgcgtcgat catttcgacc attcaggatc
     4981 gtggctatgt gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca
     5041 ccgatcgcct ggaagagaat ttccgcgagt taatgaacta cgacttcacc gcgcagatgg
     5101 aaaacagcct cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact
     5161 tcttctcgga tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta
     5221 tgcgcccgaa ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg
     5281 ggattcgcac agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga
     5341 aagagcgttg caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg
     5401 aaggtgaaga cgctgaaacc aatgcgttgc gcgcaaaacg tcgttgcccg aaatgtggca
     5461 cggcgatgga cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc
     5521 caacctgcga cggttacgag atcgaagagg gtgaattccg cattaaaggt tatgacggcc
     5581 cgatcgttga gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta
     5641 aatacatggc ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggcg
     5701 aagtggcacc accgaaagaa gatccggtgc cattaccgga gctgccgtgc gaaaaatcag
     5761 atgcctattt cgtgttgcgt gacggtgctg ccggtgtgtt cctggcggcc aataccttcc
     5821 cgaaatcgcg tgaaacgcgt gcgcctctgg tggaggagct ttatcgcttc cgcgaccgtc
     5881 tgccggaaaa actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga
     5941 ccatggttcg ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa
     6001 aggcgactgg ttggtcagca ttttatgttg atggcaaatg ggttgaaggg aaaaaataac
     6061 ctttaattcc gtctggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca
     6121 tattattttt tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag
     6181 cacctttttt attattaaat cgtattagtc acccgcctgg tgtggcgaaa aaacgatgtt
     6241 ctgatggcgt ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga
     6301 ggtggtcaat cataacctga atgtctcatc aacggcggaa ggactttaca catcacaacc
     6361 cgggatcagt aaacaagtca gaatgctgga agacgagctg ggcattcaaa ttttttcccg
     6421 aagcggcaag cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg
     6481 tgaagttctg tcgaaagtcg atgccataaa atcggtcgcc ggagagcaca cctggccgga
     6541 taaaggctcg ctgtatatcg ccaccacgca tactcaggca cgctatgcgt taccaaacgt
     6601 tatcaaaggc tttattgagc gttatcctcg cgtttctttg catatgcacc agggctcgcc
     6661 gacacaaatt gctgatgccg tctctaaagg caatgccgat ttcgctatcg ccacggaagc
     6721 gctgcatctg tatgaagatt tagtgatgct accgtgctac cactggaatc gggctatagt
     6781 agtcactccg gatcacccgc tggcaggtaa aaaagccatt accattgaag aactggcgca
     6841 atatccgttg gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt
     6901 taaccgcgca gggttaacgc cgcgtatcgt cttcacggca acggatgctg acgtcattaa
     6961 aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt
     7021 cgccgatccc gaccttgtgc gtgttgatgc tcacgacatc ttcagccaca gtacaaccaa
     7081 aattggtttt cgccgtagta cttttttgcg tagttatatg tatgatttca ttcagcgttt
     7141 tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ctaatgaaga
     7201 aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc
     7261 ggcacctttt atgtagcgaa ggtgccggaa tatattctcc tttgttactt atttgtatcc
     7321 ggtgcacaat atttttatcc ctcatattta tagggtaaat tacctgtaa
//
LOCUS       DACADA010000200         7365 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.202, whole genome shotgun sequence.
ACCESSION   DACADA010000200 DACADA010000000
VERSION     DACADA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.202"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(66..191)
                     /locus_tag="ISB17_004269"
                     /pseudo
     CDS             complement(66..191)
                     /locus_tag="ISB17_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            233..463
                     /locus_tag="ISB17_004270"
                     /pseudo
     CDS             233..463
                     /locus_tag="ISB17_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            515..1450
                     /gene="ttcA"
                     /locus_tag="ISB17_004271"
     CDS             515..1450
                     /gene="ttcA"
                     /locus_tag="ISB17_004271"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAP1982140.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1578..2951)
                     /gene="dbpA"
                     /locus_tag="ISB17_004272"
     CDS             complement(1578..2951)
                     /gene="dbpA"
                     /locus_tag="ISB17_004272"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAP1982141.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3429..4412)
                     /gene="zntB"
                     /locus_tag="ISB17_004273"
     CDS             complement(3429..4412)
                     /gene="zntB"
                     /locus_tag="ISB17_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAP1982142.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4667..5899
                     /gene="dgcM"
                     /locus_tag="ISB17_004274"
     CDS             4667..5899
                     /gene="dgcM"
                     /locus_tag="ISB17_004274"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAP1982143.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5920..6483)
                     /gene="smrA"
                     /locus_tag="ISB17_004275"
     CDS             complement(5920..6483)
                     /gene="smrA"
                     /locus_tag="ISB17_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAP1982144.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6678..7331)
                     /locus_tag="ISB17_004276"
     CDS             complement(6678..7331)
                     /locus_tag="ISB17_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP1982145.1"
                     /translation="MFLGRVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWL
                     LMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERL
                     KLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALF
                     FNRGGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         1983 a         1881 c         1704 g         1797 t
ORIGIN      
        1 aaatatcccc tgatagaaaa ctaaaaaaca ctgcataata tcgctattct ggcacttgcg
       61 aaaaattata ttctgcacct gttgtagcgt tctatttctc tgcctggcaa cgaatgcaca
      121 atgtccgcca cagtttctgt tctaatttct tcctgtgtta ccgcatcctg tacgggtagc
      181 gcaacaccag catgctaagc aaggctttcc catcagattt cactacgact atttgggagc
      241 gagatggaat gcagcagtaa aacgtgctgg tattcgccgc cgtaatccgt accatacgcg
      301 acatactttt gcctgctggc tgttgacggc aggagcgaac ccggcattta tagccagcca
      361 gatggggcat gaaactgcgc agatggtgta tgaaatttac ggtatgtgga ttgatgacat
      421 gaacgacgaa caggtagcca tgttgaatgc gcggttatcg tagttgcaaa gtttgccctc
      481 aatatgcccc atttagtacc agagaactga aataatgcaa gaaaatcaac aaattacaaa
      541 gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc
      601 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
      661 taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg cgccaatcaa
      721 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
      781 gcccgagtat cttgaaacgc tgggcgttga gtacaagatt gttgaagaga acacttacgg
      841 tatcgtgaaa gagaagattc cggagggcaa aaccacttgc tcactgtgtt ctcgtcttcg
      901 tcgcggtatc ctttatcgca ccgcaacgga actgggggcg acgaagatcg cgctgggtca
      961 ccatcgtgac gatatcctgc aaacgctgtt cttaaatatg ttctacggtg gcaagatgaa
     1021 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgtgattc gtccgctggc
     1081 ctactgccgc gagaaagata ttcagcgctt tgccgatgca aaagcgttcc cgattattcc
     1141 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtaattgctg acatgttgcg
     1201 tgactgggat aaacgttatc cggggcgtat cgagacgatg ttcagcgcaa tgcagaatgt
     1261 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta cccacggttc
     1321 tgaagtggtt aacggggggg atctggcgtt tgatcgcgaa gagatcccac tacaaccagc
     1381 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
     1441 agtgaaataa ccaggatagc gcccgatacg caagcgtatc gggctactct tatggaagcc
     1501 gataagacgc ggccagcgtc gcatccggca atgccgaaga agatgtttac tcttgcaccc
     1561 tgcaattcaa catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc
     1621 ccgccctgta actgtttcca tgctttatga gcaacagcct gacgcaccgc gacataaaca
     1681 tgcgccggat gcacggcaat tttgccaata tctgcgccat caagcccgat gtctcctgtc
     1741 agcgcaccta atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac
     1801 gttgccattt ctgcttccag cggcacaatg gaactattcg ctggcggcgt ttgccagtta
     1861 agttttatct gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag
     1921 aaactgatcg ccagaccgct atttcctgca cgagctgtac gaccgatgcg atgtacatga
     1981 acttcagggt cccacgccag ctcaaagttc accaccagct caagcgattt tatatccaga
     2041 ccacgcgcag caacatcagt cgcgaccagt acacgggcgc taccgttagc aaaacgtacc
     2101 agggtctgat cgcgatcgcg ttgctccaga tcgccgtgta acgacaatgc actttgccct
     2161 acttcattca gcgcgtcgca gacagactgg caatcttttt tggtattgca aaacaccacg
     2221 caagaggatg gctgatgcaa gcttaataac cgttgcaaca gaggaatttt gcctttgctg
     2281 gatgtctcat aaaattgttg ttcaataggt ggcagagcat ctgttgagtc aatttcaatc
     2341 gctaaaggat cgcgctgcac tcttccgctg attgcggcaa tggcttccgg ccaggttgcc
     2401 gaaaacagaa gtgtctgccg agatgctggc gcaaaacgga tgacatcatc aatggcgtca
     2461 ctaaatccca tatccagcat gcggtcggcc tcatccatca ccagcgtatt caacgcatcc
     2521 agtgatacag tgcctttttg caggtgatcc agcaaacggc ccggcgttgc cacgataata
     2581 tgcggcgcat gttgtaacga atcacgctgc ataccgaacg gttgaccacc gcacaacgtc
     2641 aaaattttgg tatttggcag aaaacgcgcc agccgacgca attcacctgc cacctgatcc
     2701 gccagttcac gcgtaggaca cagcactaaa gcctgggttt gaaatagcga cgcatcaatt
     2761 tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg
     2821 cgaacatctt ttccggcaag gatcgccgga agcgcggcgg cctgcaccgg cgtcatggtt
     2881 aaataaccca acccattaag gtttgtgagt tgggcgggag gcaaaacatt cagggtagaa
     2941 aaagcggtca caatctattc tcgtggtcat cgacgcaaag tcagcaggcg cgtatcctcg
     3001 cagatctacg ctcacgatgc gacaatttaa tcggctcttc atcgggtggt gggtcaggca
     3061 tgggttgcgg gcgagggatc ggatcgggca cgggaacagg atcgccagga atcggttcag
     3121 ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc ctccggtcat tgggtggact
     3181 cttaaagggt agacgctgat aaataacagg caaaaaaaag ccgactcatc aaagtcggcg
     3241 tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg aagtaagaca atatggagcg
     3301 caacgcccat cgcttgacgt tgcattcacc tgcaagagag atattgccct gaatgggtag
     3361 agagtttatt gacttcgctc aaactttacg gcgtttttgt atacagacag ccgggaaaat
     3421 tgcttttgtt acaaccattt actacgatgc aaccataaag caacaccacc aataagaaca
     3481 actaacagaa tacaaaaaat tgaaaatccg aattgccatc caccgccagg gatcccacca
     3541 aggttgacgc caaataaccc tgtcagaaac gtactgggta aaaagaccat tgccatcaac
     3601 gacattgtat aagtacgacg agctaaattt tcctgcatca cctgagcgat ttcatccgcc
     3661 atcacgccag tccgtgctat acaggcgtcg atttcgtcaa gaccgcgccc aaggcgatcg
     3721 gcaatatcct gcatccgacg gcgttggtca tcgctcatcc acggcaaacg ttcactggca
     3781 agacgagcat aaacatcacg ttgcggtgcc atatagcgac gcatcacaat taattgtttg
     3841 cgcagcagag ccaggaatcc acgcggtgga atttgctgat cgagaagatt atcttcgagg
     3901 tcgataattt tatcgtgcag ctgctcgata aattcgctgg aatgatcggt caacgcatcg
     3961 cacacatcaa ccagccaccc cccgcaatcg gtcggaccag tgccctcttc cagatcgctc
     4021 accacatcgt ccagcgccag cactttgcgt tgtcgggtcg aaacaattaa ccgcccgtcc
     4081 atatatacac gcatggcgac cagttgatcg gggcgttcat cggtgctgcc gtttatacag
     4141 cgcaatgtaa tcagcgtgcc ttcaccgaga cggctgactc ggggccgtgt gctctcgccc
     4201 gccagcgcat cacgtacgtt attgggaagc agcggtgttg tcgccagcca ttgggcgcta
     4261 tcatggtgta cataattaag gtggagccag cagggatgcg cttcatcaat cacatctgta
     4321 ttttccagcg gtttaacgcc gcctctacca tccagcatcc atgcaaatac tgcatccggg
     4381 acattaacgt ccgatccctt aatcgcttcc acagtgcctc catcatcaac gcattatttt
     4441 gtagtctagc cttctggccc tgttacgcaa catctcatca ccccattacc ctgaaatgat
     4501 taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg cttttccgta tacaaaccgt
     4561 gtgaagtgtt aaatagcgtc tatcattatc agaattatct gatcatatga cgtggctttt
     4621 ttgcgatcgg atagcaacaa caattgataa aaataatggg atctcaatga ttacgcacaa
     4681 cttcaatacc ctggacttac tcaccagtcc tgtctggatc gtttcgccct ttgaggaaca
     4741 gttaatttat gccaacagcg aggcgcgact gttgatgcaa gacctcacgt ttagtcagct
     4801 acgaaccggt ccctattccg tctcctcaca aaaagaactg ccgaaatacc tctccgatct
     4861 gcaaaatcaa cacgatatta tcgaaatcct caccgttcag cgcaatgaag aggaaacagc
     4921 attaagctgt cggcttgttt tgcgagagct gacagaaaca gaaccggtga ttattttcga
     4981 aggtatcgaa gcaccggcaa cgctgggttt aaaagccagt cgctcgacaa attatcagcg
     5041 caaaaaacaa ggtttttatg cgcgcttttt tctgactaac tctgcgccaa tgttgttgat
     5101 tgacccctca cgagatggcc aaatcgttga tgctaacctc gccgctctca atttctatgg
     5161 ttataaccat gaaacgatgt gccagaaaca tacctgggaa ataaatatgc tcgggcgtcg
     5221 cgtcatgcct atcatgcatg aaatctcgca tttacccggt ggtcataaac ctttgaattt
     5281 tgttcataaa ctggcggatg gttcgactcg tcatgtgcag acctatgccg ggccgattga
     5341 aatttatggc gacaagctca tgttatgtat tgtgcatgat attactgagc aaaaacggct
     5401 ggaggagcag ctggaacatg ctgcccacca tgatgcgatg acaggattac tgaatcggcg
     5461 acagttttat cacattaccg agccaggcca aatgcagcac ctcgccatcg ctcaggatta
     5521 cagcttgttg ctcatcgaca ccgatcgttt taaacacatt aacgatctct atgggcattc
     5581 taaaggtgat gaggtgttat gcgcccttgc ccgcactctc gaaagttgcg ctcgcaaagg
     5641 cgatttggtg tttcgttggg gaggcgaaga gtttgtctta ttgctaccaa gaaccccact
     5701 ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc gtggcaaaag tgagtatttc
     5761 gggcttacca cgctttactg tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat
     5821 cgatgaactg tttaaacgcg ttgatgatgc tttgtatcga gcgaaaaatg atggacgcaa
     5881 ccgcgtgctg gcggcataag ccacggatgc gtctcgtgat caacgactgc gcttagcatg
     5941 gcgctcccag ttttcttgct tcgcctgcgc tgttttacgt agtgcgacgt aacacgcccc
     6001 gctgccgcca tgatgcggta gcgcagtgca atatgcctga acatcatcaa attcggtcag
     6061 ccagcgcgcc acatagctgc ggacaatatt ggcatgcgat ttatcatccc gtcctttgcc
     6121 atgaataatc agcacgttac gcaaaccatc cgccagggct tgttgaatga aactgaacat
     6181 cattttgcgg cactcttcca ccggctggcg caaaagattc aggctcgcct gttgcggata
     6241 tttaccactg cgcagcttat ccagcacccc atgttgcaac ccttcccgcc gaaactccag
     6301 cggccgactt agcgggatga tgtcgagaaa tccagtggtg aggaaattat caagttgcag
     6361 cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga tgccagtgga catctgtagc
     6421 acgtttcagc ggctggacat cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt
     6481 catgtaacct cctatgcacc ttcacacgtc gtgattcaga cgggttaatt gttcatactt
     6541 actgcatcgt tcccttcaca gattgaaaca atactctact tcatacctgt gagtatctgc
     6601 gcaggcggag cattttatac tagcctgtta gcagtatcga acagtttagc ccgagtttaa
     6661 ttcagcacag gctccgatca ggatgattta ttcatagcag gaaccaacaa aaatgcgata
     6721 aataacagac cactgattag cagaaaggta tccgaaaatg ccatagtttg cgcctccctc
     6781 agcatcaatt gactcaataa tttggttgaa gccagtattt ccgatgtctg gtcacttcct
     6841 ccgcgattaa aaaacaaagc acttctggaa atgaagtcat tcaccgtatg gggaacagaa
     6901 accatttttt cacccattct cgaaaaatga aaattagttc gattattgag aatagagcca
     6961 cacagggcaa taccaatcgc gcccccaaga ttacgtgtta aattaaatac accagaggct
     7021 aacttcagcc gttcttttgg aattcccccc agcgttaatg ttacgatggg ggccatcgca
     7081 aactgctgcg aaatccctct aattgcctgt ggaaacagta actcctgcca tccccattcg
     7141 tgggttatag gggtaaaaag atacattgaa aacacaaatc cacccagccc cgccatcaac
     7201 agccatcgca gatttatttt tttacttaac cagaagtaaa agggaacaga aaataactgg
     7261 aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg catttaatcc gcgaacccgc
     7321 cccaaaaaca caggtattaa atatacagta gagaatattc caacc
//
LOCUS       DACADA010000201         7354 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.203, whole genome shotgun sequence.
ACCESSION   DACADA010000201 DACADA010000000
VERSION     DACADA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.203"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            62..892
                     /locus_tag="ISB17_004277"
     CDS             62..892
                     /locus_tag="ISB17_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1982146.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            924..1859
                     /locus_tag="ISB17_004278"
     CDS             924..1859
                     /locus_tag="ISB17_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1982147.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1952..2194
                     /locus_tag="ISB17_004279"
     CDS             1952..2194
                     /locus_tag="ISB17_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAP1982148.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            2184..3635
                     /gene="panF"
                     /locus_tag="ISB17_004280"
     CDS             2184..3635
                     /gene="panF"
                     /locus_tag="ISB17_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAP1982149.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3647..4528
                     /gene="prmA"
                     /locus_tag="ISB17_004281"
     CDS             3647..4528
                     /gene="prmA"
                     /locus_tag="ISB17_004281"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP1982150.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4857..5822
                     /gene="dusB"
                     /locus_tag="ISB17_004282"
     CDS             4857..5822
                     /gene="dusB"
                     /locus_tag="ISB17_004282"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP1982151.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5848..6144
                     /gene="fis"
                     /locus_tag="ISB17_004283"
     CDS             5848..6144
                     /gene="fis"
                     /locus_tag="ISB17_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAP1982152.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6229..7113
                     /gene="yhdJ"
                     /locus_tag="ISB17_004284"
     CDS             6229..7113
                     /gene="yhdJ"
                     /locus_tag="ISB17_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP1982153.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7197..>7354
                     /locus_tag="ISB17_004285"
     CDS             7197..>7354
                     /locus_tag="ISB17_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAP1982154.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDEL"
BASE COUNT         1789 a         1791 c         1900 g         1874 t
ORIGIN      
        1 gattgaagga taactttcaa agaaaaatac aaggggctat agtgttacgc attaaaggcc
       61 aatgaagagt gttaacgtgc cgagaaaatc gtctaactta ctgaaacgta gattaaaaat
      121 tgcagaaatt gttgccgccc agggtgaaat caaagttgat gacttatctg cgcaacttgg
      181 tgtctctggc gtgacgatcc gcggcgatct cagttatctg gagcaacagg gatatttaaa
      241 gcgatcattt ggcggtgcta ttgcgacacc actctcctct gaaccagaag cgatacaacc
      301 gatcgcgcca ttgtctctgg cacaacaaat ggaaatagca cgacattgcg cacggttaat
      361 tcgcgatcgt gacacgctat ttcttggaca tgggacaatc tgccgtaaga tcattccatt
      421 gctcagcgaa gtgaaaaaac tgcgtctgat caccaacgat cccgaacatg cgctgctggc
      481 aaatcagttt attgatggcg aaataatact ggctggcagt gaaatgctgc gccccgacac
      541 cgcactggcc ggaaaacagc ttgaacaggc acttcagcat ttcaccatta cacatagcat
      601 tctggaaatc agccatctgg atgctgatgg tactttaaat atcaacgttc ccagactcgc
      661 cgctgcctgg cagctctgtt tcgatcatgc ccagaataag acggttatcg ttgccgccga
      721 tcctgcgcca tcaacggctg ctatcgggca tttgcatcag gcagaaaatg tgatcgtcag
      781 ccgcagcatt aacgaacaat atcagcaaca attgcagtcg gctgatttcg ttatccttta
      841 taccagtaat gagtgcttta cctggtccaa tcgcgaaagg cttcaggaat gagaggggtt
      901 ttgtgacaat aactatctgc gctatgggcg aattgctggc cgagtttttg tcccgcaacc
      961 cacatcaaaa attcactcag cctggggagt ttatcgggcc atttcccagc ggtgcgccag
     1021 caatttttgc tgctcaggtg gcaaaactgt cccatcgggc catcttcttt ggatgtgttg
     1081 gtaatgatga ttttgcccga ctcattatag agcgtctccg tcatgaaggt gtcattaccg
     1141 atgggatcca tgttatgaac aatgccgtca caggtacggc gttcgtgagt tatcaaaatc
     1201 cccagctgcg ggatttcgtc tttaatatcc ctaacagcgc ctgcggtttg tttactgccg
     1261 agcacattga taaggatctg cttaaacagt gtaaccatct gcatattgtg ggctcatcgt
     1321 tgttctcatt tcgcatgatc gatgtcatgc gtaaagcaat aacgacgatc aaatcggctg
     1381 gcggcaccgt ttctttcgat cccaatattc gcaaagagat gctgagcatt cctgaaatgg
     1441 cgcaggctct cgattatttg attgaatata cggatatttt tatccccagc gaaagcgaac
     1501 tccctttctt cgcgcgtcac aaaaatctgt cagaggaaca gattgttagc gatcttctcc
     1561 acggcggcgt aaaacatgtg gcgataaaac gcgcccagcg tggggctagc tattacaagc
     1621 ttaaaaacgg tacattacac gcccagcatg ttgcaggtca cgatatcgaa attatcgatc
     1681 caacgggtgc aggcgactgc tttggcgcaa cgtttatcac tcttttctta tccggtttcc
     1741 cggcacacaa ggcgctgcaa tatgcaaatg ccagcggcgc gctcgccgta atgcggcaag
     1801 gtccgatgga agggatatcc tcactggcag acattgaaga ctttttgcag cagcactgaa
     1861 cggcaatttt ctgtcagatt attttccctg cctgaagact tgccgtagaa ttgcccgttt
     1921 ctcgtatccc cccatgagga ggaccccttc cgtggacact cgttttgttc aggcccataa
     1981 agaggcgcgc tgggcgctgg ggctgaccct tttgtatctg gcggtatggt tagtagccgc
     2041 ttacttacct ggcgttgccc ccggttttac cggttttccg cgctggtttg agatggcctg
     2101 catcctgacg ccgctgctgt ttattggact gtgctgggcg atggtgagat ttatctatcg
     2161 cgatatccca ctggaggatg acgatgcagc ttgaagtaat tctaccgctg gtcgcctatc
     2221 tggtggtggt gttcggtatc tctgtttatg cgatgcgtaa acggagcact ggcaccttcc
     2281 ttaatgagta tttcctcggc agccgctcta tgggcggtat tgtgctggcg atgacgctca
     2341 ccgcgaccta tatcagtgcc agttcgttta tcggcgggcc tggagctgct tataaatacg
     2401 gcctgggctg ggtattgctg gcgatgattc agcttcctgc agtctggctt tcgctcggta
     2461 ttctcggcaa gaagttcgca attcttgcgc gccgctacaa tgcggtgacg ctgaacgata
     2521 tgctgtttgc ccgctaccag agtcgtcttc tggtgtggct ggcgagtttg agtttgctgg
     2581 ttgcgttcgt tggtgcgatg accgtgcagt ttatcggcgg tgcgcgcctg ctggaaaccg
     2641 cggcgggtat tccttatgaa accgggctgc tgattttcgg tatcagcatt gcgttgtata
     2701 ccgcctttgg cggctttcgc gccagcgtgc tgaacgacac catgcaaggg ctggtgatgc
     2761 tgattggcac cgttgtgctg cttattggcg tggtacatgc cgcaggtggt ttaagtaatg
     2821 cggtagacac gctgcaaacc atcgatccgc aactggttac gccacaaggc gctgacgata
     2881 ttctgtcgcc tgcctttatg acgtcgttct gggtactggt gtgctttggc gttataggcc
     2941 tgccgcatac tgcggtgcgc tgtatctctt ataaagacag caaagcagta catcggggga
     3001 tcatcatcgg tacgattgta gtcgcaatcc tgatgttcgg tatgcatctg gccggagcgt
     3061 taggtcgggc ggtgatcccc gatctcaccg taccggatct ggtgatcccg acgttaatgg
     3121 taaaagtgct gccaccgttt gccgccggga tcttcctggc tgcaccgatg gctgcgatca
     3181 tgtcgacaat taacgcccaa ctgctgcaaa gttccgctac gatcattaaa gatctctatc
     3241 tgaatatccg tccggatcaa atgcaaaacg agacgcgtct gaagcggatg tcggcggtga
     3301 tcacgttggt tctcggtgcg ttgctgctgc ttgccgcctg gaagccgcca gaaatgatca
     3361 tctggctgaa tttgctggcc ttcggtgggc tggaagccgt tttcctgtgg ccgctggtgc
     3421 tgggtcttta ctgggaacgc gccaacgcca aaggcgcgct aagtgcgatg atcgttggcg
     3481 gcgtgctgta tgccgtactc gcgaagctga atattcagta cctgggcttc caccctatcg
     3541 tgccctcgtt actactaagt ttgctggctt tcctggtcgg aaaccgtttc ggtacatccg
     3601 tcccgcaagc taccgttttg actactgata aataaagagt tttgccatgc cttggatcca
     3661 actgaaactg aacaccaccg gcgcaaacgc ggaagatctt agcgatgcgc tgatggaagc
     3721 gggtgccgtt tctatcactt ttcaggatac ccacgatacg ccagtatttg agccgctgcc
     3781 gggcgaaacg cgcctgtggg gcgacaccga tgtgattggt ctgttcgacg ctgaaaccga
     3841 tatgaacgac gtggtggcga ttctggaaaa ccatccgctg ctcggcgcgg gcttcgcgca
     3901 taaaatcgaa caactggaag ataaagactg ggagcgcgaa tggatggata atttccaccc
     3961 gatgcgcttt ggtgaacgac tgtggatctg cccaagctgg cgtgatgtgc cggacgaaaa
     4021 cgccgtcaac gtgatgttag acccagggct ggcgtttggt acgggtaccc atccaactac
     4081 ctctctgtgc ctgcaatggc tcgacagcct cgatttaacc ggtaaaacag tcatcgactt
     4141 tggctgcggt tccggcattc tggctatcgc ggcgctgaaa ctgggtgcag caaaagccat
     4201 tggtattgat atcgatccgc aggcgatcca ggccagccgc gataacgccg aacgtaatgg
     4261 cgtttctgat cgtctggaac tctacttacc gaaagatcag ccagaagaaa tgaaagccga
     4321 cgtggtggtc gctaacatcc ttgcaggccc attacgtgaa ctggcaccgt taatcagcgt
     4381 cctgccagtt tcaggcggtt tgctgggcct ttccggtatt ctggcaagcc aggcagagag
     4441 cgtttgcgaa gcttatgccg acagcttcgc tctggacccg gtcgtggaaa aagaagagtg
     4501 gtgccgcatt accggtcgta agaattaacc ttcgcatcgc cgtatggcga cgcagagcga
     4561 agtgcgagcg atgtcacaaa aggcacgtta atttgccgat tatttacgca aatttgcgtg
     4621 ccaaaatttt cattcataaa gaaaaattga gaacttactc aaatttcttt gagtgtaaat
     4681 tttagtcact attttctaat atgatgattt ttatgagtaa ttatcgcgct acgctcattt
     4741 taaatgcaat tctttgatcc atctcagagg attggtcaaa gtttggcctt tcatctcgtg
     4801 caaaaaatgc gtaatatacg ccgccttgca gtcacagtat ggtcatttct taactcatgc
     4861 gcatcggaca atatcagctc agaaatcgcc tgatcgcagc gcccatggct ggcattacag
     4921 acagaccttt tcggacgttg tgctacgaga tgggagccgg attgacagta tccgagatga
     4981 tgtcttctaa cccacaggtt tgggaaagcg acaaatctcg tttacggatg gtgcacattg
     5041 atgaacccgg tattcgcacc gtgcaaattg ctggtagcga tccgaaagaa atggcagatg
     5101 cagcacgtat taacgtggaa agcggtgccc agattattga tatcaatatg ggttgcccgg
     5161 ctaaaaaagt gaatcgcaag ctcgcaggtt cagccctctt gcagtacccg gatgtcgtta
     5221 aatcgatcct taccgaggtc gtcaatgcag tggacgttcc tgttaccctg aaaattcgca
     5281 ctggctgggc accggaacac cgtaactgcg aagagattgc ccaactggct gaagactgtg
     5341 gcattcaggc tctgaccatt catggccgta cacgcgcctg tttgttcaat ggagaagctg
     5401 agtacgacag tattcgggca gttaagcaga aagtttccat tccggttatc gcgaatggtg
     5461 acattactga cccgcttaaa gccagagctg tgctcgacta tacaggggcg gatgccctga
     5521 tgataggccg cgcagctcag ggaagaccct ggatctttcg ggaaatccag cattatctgg
     5581 acactgggga gttgctgccc ccgctgcctt tggcagaggt taagcgcttg ctttgcgcgc
     5641 acgttcggga actgcatgac ttttatggtc cggcaaaagg gtaccgaatt gcacgtaaac
     5701 acgtttcctg gtatctccag gaacacgctc caaatgacca gtttcggcgc acattcaacg
     5761 ccattgagga tgccagcgaa cagctggagg cgttggaggc atacttcgaa aattttgcgt
     5821 aaacagaaat aaagagctga cagaactatg ttcgaacaac gcgtaaattc tgacgtactg
     5881 accgtttcta ccgttaactc tcaggatcag gtaacccaaa aacccctgcg tgactcggtt
     5941 aaacaggcac tgaagaacta ttttgctcaa ctgaatggtc aggatgtgaa tgacctctat
     6001 gagctggtac tggctgaagt agaacagccc ctgttggaca tggtgatgca atacacccgt
     6061 ggtaaccaga cccgtgctgc tctgatgatg ggcatcaacc gtggtacgct gcgtaaaaaa
     6121 ttgaaaaaat acggcatgaa ctaattcagg ttagctaatt gcttgattaa aaggcgctac
     6181 tcggcatggg gaagcgcctt ttttataggt gtcacaaagg gagtgaccat gagaacagga
     6241 tgtgaaccga cccgatttgg taatgaagca aagaccatca tccagggtga tgcattaacc
     6301 gaacttaaaa agctccccgc cgaaagtgtc gatctgatct ttgccgaccc accgtataac
     6361 atcggtaaaa attttgatgg tctgatcgaa gcctggaaag aagatctgtt tatcgactgg
     6421 ctgtttgaag tgattgcaga gtgccatcgc gttctgaaaa agcagggcag catgtacatc
     6481 atgaatagca cggaaaacat gccctttatc gatctccagt gccgcaagct ttttaccatc
     6541 aaaagtcgca tcgtctggtc atatgacagt tctggagtgc aggcgaaaaa acactacggc
     6601 tccatgtacg aacccatcct gatgatggtg aaagacgcaa agaactacac gttcaacggt
     6661 gatgctattc tggtagaagc caaaaccgga tcgcagcgcg cgttgatcaa ttatcgcaaa
     6721 aatcctccac agccttacaa tcatcaaaaa gtaccgggta acgtctggga ttttccgcgc
     6781 gtgcgttatt tgatggatga atatgaaaac cacccgacgc aaaaaccgga agccttgctg
     6841 aaacgcatta ttctcgcctc ttccaaccca ggcgatatcg ttctcgaccc gtttgccggt
     6901 agctttacta ccggtgccgt agccgtcgcc agcgggcgaa aattcattgg tattgagatc
     6961 aacagcgagt acatcaaaat ggggcttcga cggctggatg tcgcgtcgca ttactctgcg
     7021 gaagaactgg cgaaagtgaa aaaaagaaag accggcaacc tgtcaaaacg aagccggtta
     7081 agcgaagttg accccgatct cattgcaaag taaaggaagt gtaagcctgg ttttcagatt
     7141 attcatttcg tgtatatttc ctgccagact tggttaaaca tgcacaggct caggtaatga
     7201 ttcgcaagta ttggtggctc gtcgttttcg ctgtcttcgt ttttctgttt gatactttac
     7261 tgatgcagtg gattgaactg ctggcaacag aaacagacaa atgccgcaat atgaactcag
     7321 ttaatccact aaaactggtt aactgtgacg aact
//
LOCUS       DACADA010000202         7315 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.204, whole genome shotgun sequence.
ACCESSION   DACADA010000202 DACADA010000000
VERSION     DACADA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.204"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            237..1769
                     /locus_tag="ISB17_004286"
                     /pseudo
     CDS             237..1769
                     /locus_tag="ISB17_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1713..2312)
                     /locus_tag="ISB17_004287"
                     /pseudo
     CDS             complement(1713..2312)
                     /locus_tag="ISB17_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            2332..2654
                     /locus_tag="ISB17_004288"
                     /pseudo
     CDS             2332..2654
                     /locus_tag="ISB17_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3232..3447)
                     /locus_tag="ISB17_004289"
     CDS             complement(3232..3447)
                     /locus_tag="ISB17_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982155.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(3451..3810)
                     /locus_tag="ISB17_004290"
     CDS             complement(3451..3810)
                     /locus_tag="ISB17_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982156.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHEV"
     gene            complement(4101..4373)
                     /locus_tag="ISB17_004291"
     CDS             complement(4101..4373)
                     /locus_tag="ISB17_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982157.1"
                     /translation="MQNNKKQGHIMKDEIHSTSINPDVVEQFLALMNTHCKSSEEALA
                     MLSTLCYNLMVTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            4828..4987
                     /locus_tag="ISB17_004292"
                     /pseudo
     CDS             4828..4987
                     /locus_tag="ISB17_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5205..7295)
                     /gene="iha"
                     /locus_tag="ISB17_004293"
     CDS             complement(5205..7295)
                     /gene="iha"
                     /locus_tag="ISB17_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HAP1982158.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT         1865 a         1822 c         1784 g         1844 t
ORIGIN      
        1 tactttggac tattactcct gaacaactgt aagcgtacag cctgaaccgt ctggtcagaa
       61 tctgacgaat tagacaaagt ggtgtccacc aaataagtag tgggaaccaa agtgtcagat
      121 atgcagaaaa atgtgactcc cggcaggcga aaaggctgcc ctaattatcc tcccgaattt
      181 aaacagcagc tcgttggggg atgcggacga tttcctggac tctcaggaga taaaacatgt
      241 ttactgaaga agaaaaaatc cgcgccattg agctttattt caaatatggt aagaagctgg
      301 ctcccgtcgt acgcgaactg ggctaccctt cgaaaagaaa tctgcggcgt tggatccgct
      361 catgggaagc aggtggtggc gcaaaagaat ccattcgtca taagcaccgt tactccgatg
      421 agcaaaaaca ggttgccgtt gaacattatc ttaaccacgg ctgctgcctg gcgtttacca
      481 gcagagccct gggttatccc tgcactgatg tactcgctcg ctgggttaat gaactttatc
      541 ccgacagacg acgtatattt accagtaagg ccaacccggt tgcgcccttt gaaccagaag
      601 tcaaacgtca ggctgtcatg gcactctgta cacgacaagt atcagccagt gagatcgcca
      661 ggcgtattgg tgtcagtcgt gcggtattgt ataaatagaa agatgaaatt atcggcaaca
      721 gtgcttacca gactatgcgt aaacataacg aaccttccct ggaggcagaa cgcgatgcgt
      781 tgcgggagga agtcgcccga ctgaatcagg aaatacgccg ccggcagatg gagctggata
      841 ttctgaaaaa ggcggaggaa atcataaaaa aagacccggg catcagtatc agtcacctga
      901 acaacagaga aaaaacaaag atcgctgatg ccctgaggca aacatatccc ctgacagaat
      961 tacttcatgt tctgggcctt acccgtagca gttattttta tcaccgggct gcactgaaag
     1021 ccggtgataa atacgccacg atacgtacga tgctgacaga tatatttaac agtaattacc
     1081 agtgttatgg ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag
     1141 aaaaggttgt acgcagactt atggtggagg aacagcttgt cgtcagccgt aaccgtcgtc
     1201 gtcgctacag ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag
     1261 attttaaggc gtagcaacct aatcagaaat ggctgacaga tatcacggag ttccaactcc
     1321 ctgcaggtaa agtctggctg tcaccggtgg tggactgctt cgatggaaaa gttattagct
     1381 ggtctctcag tacacgcccc gatgctgagc tggtcaacac tatgctggat agcgctgtcg
     1441 aaacgttaaa tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct
     1501 ggccaggctg gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag
     1561 gatgttcacc tgataatgcc gcatgcgaag gttttttcgg cagactgaaa acggaaatgt
     1621 attatgggcg taaatggtcg ggcatcacgc cagaaaagct catgcagcat gtagatgctt
     1681 acatcagatg gtataacgag cggcgtataa aattatcgct gggtgcagtc agccctgaaa
     1741 tgtaccgcca acaatgtggg ctggcataat aaagcagtct aggaaatcgt ccgcatcccc
     1801 aattcagggg cggttttttc tttacggttt ttcgccataa agactattac tatcagactg
     1861 acaagactac ccgcgaaagc cgggagttcc gcgccaagat taacagccac cagatactgg
     1921 ggaattaatg tactgacacc acacatcagg gtaattagtc caacgcctcg tatagtcttc
     1981 ataccaccac cgataatcgc gacgatgacg aaaggaagca gaatgttgaa cagtgccagt
     2041 tgaagaataa ttgtcccgct gagtgtggtt acaggtaaat taacttgttc agcaagtaca
     2101 gagacaggta tacctactgc cccaaacgct gtagggactg tattagcaac cagtgatgca
     2161 atcgctgctt ttagtgggtt aaagcccaga gcaatcagta taccaatggg gattgctacc
     2221 gctgtaccgt atccggctgc cgcctcaaga aatccaccaa aacaccatga gataagaagt
     2281 acctggatac gtttgtcatc actaacactc gctcaatcat ttggtctgct atctcaacag
     2341 attgctttca acattattgt cagaatctgg agcagattga agatcgcata cttcaactcg
     2401 atcaggaaat atctcgcctg ttacctgagt gatctctgtg caatctggtc tgccagttgc
     2461 aggctcttaa aggtgttggt caactaatcg caataaccct ggttgctgaa ctgggtgatt
     2521 tttcgcgatt ttccaatcct gaacagctca tggcttttct gggactggtg ccaggtgaat
     2581 attccagcgg aaacagcatt cgccccagag gaataacaaa agttggaaac agcgaactga
     2641 gacatctgct ttacggatat caaataaatt cagattattt aacagtaaaa ccccacttat
     2701 gtgatgaatc tcataaaaaa gcaaagtgaa gtaataatat ccaatttatc cctcattcct
     2761 taagtaagcg tacactggcc gcgtacacca tggcataatg atatgttgtt ttactgatat
     2821 ttccggctgg taaaatcccc gacggcccgg ctgaagctgg tgcaggaaag cgggttaatt
     2881 gtcactacgc gggataacct gtctggctgg atacaacgta ttgccacaga tatgcagcaa
     2941 ctgcgtcagt ccggaatgtc agtcatctcc ggggatgttg gggtctcagg cacactgacc
     3001 ggccccccgt gttgtttccg ggtactgttt ttgatttatg caacaaagta ttgcagcgtc
     3061 gaatatgagc tatggccaga tttttgttaa tcgtggttgt tgcaacatgt aaggcgaatg
     3121 accacactat tctggcacgc tcgtaatcac gctgaaattc cgcttcctgt gctgccgtga
     3181 agagttccga ccaggacata cggcgaagtg tatgtctgtc agtaatatcc gtcatgagct
     3241 ctcagcttcc tccttcgtat ttccgtcaga aacacaaacg ccccgcagag aaaattcgcc
     3301 aacattctcc tttttaatgg cgtgcaatgc atcgcgacag gcccgttcgg tatgaaatgc
     3361 ctggctgtgc accgccagtg tgggctgccg ggcatcaccg gaagagacat taaagggaga
     3421 tgaaagccag aaaatcagta gccacatgcg tcacacctcg tgcgggtatc tggccagaat
     3481 attgagcgcc tgtaatggag cttcatgcat ttcacggata caacgggcat cggtgatgtt
     3541 ttccctgtaa tgcgtggttt ccagcatcat atgatgcata tgcagcatcc gggccagggc
     3601 tgcacggagt tcttcaatat gttgttcatg ggaatgctga cggggtggta tccggcagag
     3661 agggataacc tccacatcat ccgggtgcgc gcgaagccac gcgtcagtga ctgtaatgtc
     3721 gtcagtgtaa aaccatgtgc cggatgtccc gttggccaga aaacgcattt tccaggcttc
     3781 tggggtggcg gttgtgctgt tatctttcat ggtgccacca ctcattcaac aggcttaatg
     3841 ctgtccaggc gcagtctgct cagactgtcg tggctgccac agcggcgtcc tgtacttttg
     3901 ctgtagcact cctgccccct gatacaccat gccgttggcg tttcgtagag gctgacacgt
     3961 cgggttttat ctttactggc tagaagcccg gtgtgagttt ttgtcttttt catcggtggg
     4021 gtacaaacgc agaaattttg ttgtcatcag tttttgcctg cagaggcctg aaaagacagc
     4081 gttttcagac cctgttcagg ttagtgaata ttcttacctg gcctttcggc aatatcaatc
     4141 ccctgaagtt gcatggatat cccatgccca ttgagcatct ggctgtccat ggaatggata
     4201 ccgcatgtca ccatcaggtt gtaacaaagc gtactcagca tggcgagcgc ctcttctgag
     4261 gatttacaat gtgtgttcat aagggccaga aactgctcaa caacatccgg gttgatgctg
     4321 gtactgtgta tttcatcctt cattatgtgt ccttgttttt tattattttg catttcagaa
     4381 aataacacga tgcatgtaac ccagaaaacc gatatgtgca cgcgtagtca ctttctggca
     4441 gattccggtc tctgaaacaa aatacgaggt ggcatgaagc ggattgtggc ggacagggaa
     4501 agtgatttga ttccggggaa ggcgctgtgc agcagtctta tccggcagtt taaaggggtg
     4561 gacggagacc aatgaggcac tggagcagat tcagtgcaga ggtgacatga tttatcaaac
     4621 ttaattgtga caaaatggcg attgagaagg tgcaattgac ttatccggat tcggtcgtgg
     4681 cgaggtggtg accctgcatt ctgctaaccg ggttatgagc caggagtcag cgatagacaa
     4741 agggacttat ctgaccgggc ccccggaaaa atggcgggag agaatgatac cttcctgctg
     4801 acgagaccgg actttgagat gacgagcgat agtgcggcag tgatgtacag cctgatcggt
     4861 agctgcaaaa cttaacggaa tcgagccgga aacgtggcta cgccacgtga tcagtgtaat
     4921 caacacctgg cctgccaacc gcatgaaaga gttgttgccc tggaacgtca ctctctctgt
     4981 acactaattt ttaccccacg tccttcacgg tgcgcttaca ctctggacaa gtatgtaaat
     5041 gataagttaa aaataatccg tgcatttcct gtgacgatat tagttgttgg taacgaaatc
     5101 tcattagcgg atcggaaatg cacttaacct aatacgttgc cactgttccg ccaggagtta
     5161 cagtggcaac gtattctacc gtcagtgata gcgttttgtt attatcagaa ctgatagttc
     5221 agcgacatcc agtaatttcg ctcaggtatc acatatcctg ttgttgatga tcccgtctgg
     5281 aagtaatcac cggcatacag cgtactctta ccggcactgt acaggctcac gtcactgtaa
     5341 tccttgttga gcaggttatt caccgcagca ttcagcgtca gggcatccgt catcttccac
     5401 gacagacctg catccaccac cgtccaggct ttcaggtatt ctcctttctc atcatacact
     5461 ttcttctgta cagcgctcag tgacgaataa ttctgggtga aacgtggtgt tttcccgcga
     5521 taacgggcac ccagccatga tgccacctct tcggtgatct gccagttcag tttcgcattc
     5581 accatgtgtt caggggtata actcagcggc gcacctttgt tatcaccatc acgttgttca
     5641 cttcgggtcc aggtgtaatt cagtgacagc gtgacatcct ctgaccacag cggcaatgtg
     5701 ccggcaaatt ccacaccatg caaccgggcc tttccgctgt ttacatagct attggtgtta
     5761 tcatttatgg aataagagac aatcttgttg gagaagtcag tcataaaacc tgtgacattg
     5821 gcattcagac cggcggggtt atcgtaatac accccagcct cataactgac gctctcttcc
     5881 ggcttcaggt cggggttacc aagtagattt gtttttccct gcccggatac accactaatc
     5941 cctttatgta gctgccccat tctgggtgcc ttatatcccg tggtcacacc gcctttcagc
     6001 gtccaggcat ctgccacatc ccagaccaga tatgcacgcg gactgaagtg tcccccgaat
     6061 tgctcatgat gttcatagcg gctgcccaca gtcagcgcaa gtgcatccgt gagatgccac
     6121 tcatcctcag caaataccga ccagcttttc tgccggaaag tttcacctgt gctggcaagg
     6181 acaactccgt ctttcatgga cgagctctga aactcgcccc ccaccgtaac cagatgagat
     6241 tctcccagag gggtaagcag taatgaattc aggataaggt tcgattcctt aagctcccgc
     6301 ggctgaccgg caagccccca tttgtcgcgc ttcagtacac tgcgtacgag ctcacgacct
     6361 ttattttctg tctcgttcca gttcagatac gatttccatg ttccgaaggt gaaagtatga
     6421 tcatagccag ctgaaatttt gtttcgctca tagcgcaggg tccggtcata tccccccgtc
     6481 agactcccca gttgcccatc ccggttatca taacgctgcc gggtggtatc catatcaaac
     6541 cagagcacat cctgcgccga cgccttccag tcaagacgtg caccaagatt ataattctgt
     6601 gactccgtgg gataaggaat acgcgtggct gctgtatcgc tcagtgatgt gaccgatgag
     6661 ccctgacgct gttgtgtgct accgcgtacc tgcaggctga cagaatcatc cacaagggga
     6721 ccactgctcc agaaattaaa ctggctgctg ttaccccatt tgttgctttc ctgcagattc
     6781 agccctgcat tgacggaaga gagccatttg tctgcattct ttctggtaat gatattcacc
     6841 acaccgccca tcgcatcaga gccatacagt gtggacatcg gccccctgat aacctcaata
     6901 cgctcaatgg cggccagagg gggcatgaac ccggtattca tggcagaaaa accgttggga
     6961 gtcacgtcac tgcttccgcc ctgacgaaca ccatcaatca gtatcagcgt gtaactggct
     7021 ggcattcctc ggatgctgat ttccagccca ccggttttac ccgtaccact ttcaacatcc
     7081 acaccctcta ctgatctcag agcctccgcc agatcgtggt actggctgga ctgcaattcc
     7141 tcctggctaa tcacagaaac actggcggct gcgttagtca gctttttctc atagccggat
     7201 gcagagacaa tcatcacatc ctctgcagca gctatgctgc tggctgaaaa tccgagacag
     7261 ggaatgacta cggaagccag agtggttatt cgcatattac taatctccga tcatg
//
LOCUS       DACADA010000203         7234 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.205, whole genome shotgun sequence.
ACCESSION   DACADA010000203 DACADA010000000
VERSION     DACADA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.205"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            90..1199
                     /gene="anmK"
                     /locus_tag="ISB17_004294"
     CDS             90..1199
                     /gene="anmK"
                     /locus_tag="ISB17_004294"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAP1982159.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1297..1626
                     /gene="mliC"
                     /locus_tag="ISB17_004295"
     CDS             1297..1626
                     /gene="mliC"
                     /locus_tag="ISB17_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAP1982160.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1685..2341
                     /gene="pdxH"
                     /locus_tag="ISB17_004296"
     CDS             1685..2341
                     /gene="pdxH"
                     /locus_tag="ISB17_004296"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAP1982161.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2470..3744
                     /gene="tyrS"
                     /locus_tag="ISB17_004297"
     CDS             2470..3744
                     /gene="tyrS"
                     /locus_tag="ISB17_004297"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP1982162.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3806..4666
                     /gene="pdxY"
                     /locus_tag="ISB17_004298"
     CDS             3806..4666
                     /gene="pdxY"
                     /locus_tag="ISB17_004298"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAP1982163.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(4710..5315)
                     /gene="gstA"
                     /locus_tag="ISB17_004299"
     CDS             complement(4710..5315)
                     /gene="gstA"
                     /locus_tag="ISB17_004299"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAP1982164.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5421..6923)
                     /gene="dtpA"
                     /locus_tag="ISB17_004300"
     CDS             complement(5421..6923)
                     /gene="dtpA"
                     /locus_tag="ISB17_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAP1982165.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         1826 a         1790 c         1891 g         1727 t
ORIGIN      
        1 caacggtaaa aattggtcat tccgcgtggt attcacgaaa agtttatgtt tgagtggtgc
       61 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
      121 caggcaccag ccttgatggt gttgatgttg tgttagcgac aattgatgaa caccgggtcg
      181 cacagctggc aagtttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
      241 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
      301 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
      361 tcgttgcgat cggctgtcac ggtcaaaccg tctggcacga accgacgggc gtggcaccac
      421 acactttaca gattggcgat aacaatcaaa ttgtggcgcg caccggaatt acggttgtcg
      481 gtgattttcg ccgtcgcgat attgccctgg gaggacaagg tgcaccgctg gtacctgcgt
      541 tccatcatgc gctgctggct cacccaaccg agcgacgaat ggtgctcaat attggcggca
      601 tcgccaatct gtcactgctc attcctggcc agccggttgg gggctacgat accggtcctg
      661 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
      721 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
      781 catatttctc gcaacccgca ccgaaaagca ccggacgcga atactttaac tacggctggc
      841 tggagcgcca tttgcgccat tttccggggg ttgatccccg tgatgtgcag gccacagtgg
      901 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
      961 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
     1021 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
     1081 cattggcgtt cgcctggctt gcctggcgaa cgctggcggg attaccagga aatctgcctt
     1141 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccccgct aacccgtgat
     1201 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
     1261 gcgtattgcc ctccagacca ggaaagtctt cgggatatgt ctatgaaaaa actgttactg
     1321 atctgtttgc cggtgcttct caccggctgt agcgcgttca atcaactggt tgagcgcatg
     1381 caaaccgata cgcttgaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
     1441 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
     1501 tcagcgtctg gtgcacgtta caccgacgga atctatgttt tctggtcgaa aggcgaggaa
     1561 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccgcag
     1621 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
     1681 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
     1741 aggcgggtta cgccgccgcg atctacccgc cgatccatta accctttttg aacgttggct
     1801 ctctcaggct tgtgaagcca aactggctga ccctaccgcg atggtggtcg ctaccgtgga
     1861 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
     1921 ggtgttttac accaacctcg gcagccgtaa agcacatcaa atcgaaaata atccgcgcgt
     1981 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcgga
     2041 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
     2101 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
     2161 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccaa gtttttgggg
     2221 tggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
     2281 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
     2341 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
     2401 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
     2461 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
     2521 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
     2581 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     2641 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     2701 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacactga agaaactgtt
     2761 caggagtggg tggacaaaat ccgcaagcag gttgccccgt tcctcgattt cgactgtgga
     2821 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     2881 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     2941 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     3001 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaatcggt
     3061 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     3121 aatcaggtgt ttggcttgac cgttccgctg atcaccaaag cagacggcac caaatttggt
     3181 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     3241 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttc
     3301 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
     3361 cgcgcccagt atgtgcttgc agaacaggtg acacgtctgg tgcatggtga agaaggtttg
     3421 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ccttgagtgc gctgagtgaa
     3481 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     3541 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     3601 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     3661 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     3721 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     3781 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     3841 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
     3901 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggaccggttg
     3961 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     4021 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
     4081 cctcggtatc gttcgtcagg tgaaagcagc gaatccacag gcgaaatatt tttgcgatcc
     4141 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     4201 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     4261 ctgtgagcat ccggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
     4321 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     4381 ttttgaaatg ctgctagtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
     4441 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     4501 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtatacga
     4561 aatcatggtg accacaaaag caatgcagga atatgagctg caagtggttg ctgctcagga
     4621 tcgtattgcc aatccagaac attatttcag cgcaacaaag ctctgaaata tctcatttcg
     4681 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
     4741 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
     4801 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     4861 gtgaatcgtt gcccgcagat ccagtgctca tccttcaggg cctcgttcac atattgcagc
     4921 ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc agggcgaaac
     4981 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     5041 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     5101 tactgcataa tcgctacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggt
     5161 acctgtccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     5221 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     5281 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     5341 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
     5401 taaatgagtt tccctgaatt ttacgctacg gctgctttcg ccgctttgtc tgcagcgtca
     5461 tcctgcgtca tgcggtgcag tttcggcgcg gtaaacagca tcagtactgc aataacggca
     5521 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcggatcg
     5581 gtaacgttat ccggcacagc catcatacct gcaacataac caccaatcag gtttgcaccg
     5641 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     5701 gcaaccattg ccagacccag gccagagatc atcagttccc cgatactctg caggccatag
     5761 ctcgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
     5821 ggcagaatca ggaacgcacc agaacacatc accatgccga tagcaaactt ggttggcatc
     5881 ggcagggtat cgcccatctt gttatagata gcggccagaa tcgggctacc aatgatgatc
     5941 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccaggcccag aatggtgtgt
     6001 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctata aagcacgaag
     6061 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     6121 ttcatcgcga aggcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
     6181 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
     6241 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
     6301 ccgtactgtt taacccaacg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     6361 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
     6421 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     6481 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     6541 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
     6601 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     6661 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     6721 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     6781 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     6841 gagaagatga gatagaacgc cttcggttgt ttaaaagcgt tcaaactgac gctttcagtt
     6901 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     6961 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     7021 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     7081 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     7141 aacgtaacgg cagatttttt cactaccctg agagggtgat gatggcttaa cggcgatatg
     7201 ttctactatt tagcgttttt cctagtgaga tagt
//
LOCUS       DACADA010000204         7215 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.206, whole genome shotgun sequence.
ACCESSION   DACADA010000204 DACADA010000000
VERSION     DACADA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.206"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            159..590
                     /gene="osmC"
                     /locus_tag="ISB17_004301"
     CDS             159..590
                     /gene="osmC"
                     /locus_tag="ISB17_004301"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAP1982166.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(780..3179)
                     /gene="dosP"
                     /locus_tag="ISB17_004302"
                     /gene_synonym="pdeO"
     CDS             complement(780..3179)
                     /gene="dosP"
                     /locus_tag="ISB17_004302"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAP1982167.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3204..3421)
                     /locus_tag="ISB17_004303"
                     /pseudo
     CDS             complement(3204..3421)
                     /locus_tag="ISB17_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3745..5064)
                     /locus_tag="ISB17_004304"
     CDS             complement(3745..5064)
                     /locus_tag="ISB17_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAP1982168.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5195..6730)
                     /gene="gadC"
                     /locus_tag="ISB17_004305"
     CDS             complement(5195..6730)
                     /gene="gadC"
                     /locus_tag="ISB17_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAP1982169.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(6886..>7215)
                     /locus_tag="ISB17_004306"
     CDS             complement(6886..>7215)
                     /locus_tag="ISB17_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1982170.1"
                     /translation="AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
BASE COUNT         1849 a         1756 c         1791 g         1819 t
ORIGIN      
        1 tatctcttgg gttatatatt tccggttatc tattgtggaa atttaattta agtgcagaaa
       61 ttatattttc gccggatttt attcggatta tcctgcttat cctcgtgctg tttctcacgt
      121 agtctataat ttccttttaa gcccacagga gagcaacaat gacaatccat aagaaaggtc
      181 aggcacattg ggaaggcgat atcaaacgcg ggaagggaac agtatctact gagagcggcg
      241 tgctgaacca acagccgtat ggatttaaca cgcgttttga aggcgaaaaa ggaaccaacc
      301 ctgaagaact gattggcgca gcgcatgccg catgtttctc aatggcgctt tcattaatgc
      361 tgggggaagc gggattcacg ccaacatcga ttgataccac cgccgatgtc tcgctggata
      421 aagtggatgc cggttttgcg attactaaaa tcgcactgaa gagcgaagtt gcggtgccgg
      481 gtattgatgc ctccaccttt gacggcatta tccagaaagc aaaagcagga tgcccggttt
      541 ctcaggtact gaaagcggaa attacgctgg attaccagtt gaaatcgtaa agcgttgccg
      601 gatgacgcgt caggcgcgtg aatgcctgat gcgtcagcac aatcacatca ggctgagaag
      661 ggattgttct gacatggcta tgcgttgtcg ttgtccaact aaacgtagcg gaaatcacag
      721 catttaagtg acagtgtcac gttaaataaa aacccgcgag tgcgggcgag aggaatttat
      781 cagattttca gcggtaacac gctgctcatc cagcctggaa tttcttcggc gggcaggggg
      841 cgggaaaaga aatatccctg aataacgcga cagtggatct tgcgtagcat ctcaaattgc
      901 tctttggttt cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta
      961 atggcttcga gtaaggcaag gatgcgtttt tcagtcagac aacgatcgac aaaacttttg
     1021 tcaattttga tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgta
     1081 ccaaaatcat ctaccgataa accgatgccc atatcgcgca ggatctgaat gcgcttaaag
     1141 atttcggtat cgtgttccat catcatgctt tccgtgattt ccaccgtcag ctggtggccg
     1201 tcaatacccc aggcgtgcat tgcatcagat acctgattag gcagctgatt actgcgaaag
     1261 tgcagcgcag acaagttaac ggataacgcc gggatatgaa tattctggct acgccattct
     1321 gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc
     1381 tcttctgcga gaggaataaa ccgtgaaggg ggcacatgac catgcagggg atcgtaccag
     1441 cgagcaaggg cttcgatgcc gtacagttca cccgtttctg cgaagatttg cggctgataa
     1501 accagtttaa gttggttatt gctaatcgct tcttttagcg ctgcgcctaa aaccaaacgc
     1561 tcttttacca tttcgttcat cgccgggctg aagaactgcc agccgttacc gccatttttg
     1621 cgaataaaat ccattgcatt gtgagcagtg gagagcaggt aatcgcggtt tttacccacg
     1681 tcgtagctga tgccaatgct caaggtgagc gggaatggtt tatcgtcaat cattatcggc
     1741 ttgctgacca cattccgtag ctcatcggcg atctgggtaa tgttactgac atcgttttcc
     1801 aggctcacga ggacaaaaga cgcaccttca atacgacaga gatactgatc cggtttgagt
     1861 ttttcacgaa agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg
     1921 ctatcaatca catcctgaat atggtcaaca ccgatgagat acaccaccgg agagacggct
     1981 ttgtcgacca ggtcatcgag gtaattgtgc aggttattgc gatttggcag accggtcatc
     2041 ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt tttcctgttc cagcgccagc
     2101 gcggccatat gctggctgat atctgccacg cgttcgataa aggcgctggt ttctgctcct
     2161 gacgaggttt taatttgcag gatccccgca ggcgcgccat cgcgctgacg aatggtcgct
     2221 gaccagcttt gcgcattttg aacttctgca ccgtgcgaag atgacgccca gtgtatcggc
     2281 atcccgttgc gcagcgcgaa cagagaaaca tgcgattcgt tgagaacaga ttcgatatta
     2341 cgacaaatga tttcccccat ttcatgaaat ggtgggctgc tgcacatggc ggcgagaata
     2401 ttgccttcaa gctggcggat ttgcctttct tcggtgatat ccgagaatgt cattaccagg
     2461 ttctgcagat gcgcgagcac gtcataaacc gggctgatag aggctttaat ccagattttt
     2521 tcaccggtgc gcgtcaacag cagaaattcg tcctgatcgc gggcggtttt ccatagcaac
     2581 tgctgtaaac gaatacggtt atcagcaggg aattctggaa tgttcaggag tgtatcgggc
     2641 tgcataccgc tggcttcgtt aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac
     2701 tgcacaatat ggcgttccgg atcgaggaca atcaccggtc ggtcgagatg gtcaacggca
     2761 ataatcaatt gtcgggtctg ttctttttgc gccatttcta cgctggcatc ccgtaccagc
     2821 gccaggtaat aaactttccc ctcggcgctc actttcgata gcgcaaaacg ggtccagatt
     2881 ttactgccgt cttttttctc cagctgcagc tcccgactca tcccctcgac gcgcgcttta
     2941 ccaccttcac ggttgtgacg aatgtattga ggatgcgcag gacgcaaatc ccgcggaatc
     3001 agcatatcaa tgttattgcc aatgacttct tcacgtttgt acccccagag cttctctgcg
     3061 gcggggttga aaaacatcac ttcatcattt tcgttaatta acaccgcgcc catcatattt
     3121 tgctcaaggg cggggaaaaa aatgccatcg gcggcattat ccgcatcggt tagcttcatg
     3181 attacctctg catcctggcg catctaaaga ctggctttac agagttcaac acggtttcta
     3241 cctcgtcttt tgcgatatac agagcttcat cggctatttg aatgaggcgc tcatagtcag
     3301 gatgaccatt aaacatggcg gcaccgatgg aaagtgagag ggcaatatcc tcgccgtttg
     3361 cggctttaaa ataatgtcga cctttatggt taaaccgcag gccaaaatca accaaacaca
     3421 acgtgcggga ttggctaact gcaccatatt ctcctgaaat atgaagatat actgaaaaga
     3481 aataagcgac ttaggacagt ttcaatctac gctactgttc ttcagaagag tatagcccat
     3541 cgtaattatt tttcggtgac agcgaatatc gtatgttttt tcatattcat acatttttat
     3601 tagggggatt atggctgttt aactaagtgt ggttaattgc gtggttaatt tgacgtattt
     3661 aagcatgatt gctagtggga tagttcaaga gggtaacaag ccagtgggta aagcaccggc
     3721 ttgttacaaa gtaagaatgg gagtttaact gccccaacga ctttgcagat agctgaccgc
     3781 ttgttgagtc tgcggtttat tcagatagtc ctcacggaac aagattgtgc cgctaatttc
     3841 cggcacagca tcgttaagat cgagctgctt tttcagttcc ggtacgccgc cgttaatcat
     3901 ccagtctggc tctatctttg acggttctcc tactttatag aaggcgatac cgatatacag
     3961 gcgggtcctg gtcggtttaa cgacatccgc ccaccatttt gccaacacgt cataacgcgc
     4021 ggcactccgt gagaatggcc agtaaatttg gggagcaatg taatccagca acccttgttc
     4081 cacccatcga cgggtatcag cgtaggattc gtcataggct gccgcgcctc gggtatcgga
     4141 accgagcgga tcgtgtgatc ggttacgcca cacgcctgcc gggctaacgc caaattcgac
     4201 ttccggctta atgcttttaa tggtgtgcga aaccttcgca attaactgct gggtattgtt
     4261 gcgccgccag tctgcttttg acgcaaatgc gcctccgtat ttgcggtacg tttcgttatc
     4321 atttagccgt gaacctggtg actcagtata gaaatagtca tcaaactgca cgccatctac
     4381 cggatagcgg gaaaccactt ccgcaactat tgatgtgatc cagtcctgaa cctcagggat
     4441 gcccgggtcg aggacaaagc gatcgccgga cgttctgatc cagtcgcggt gttgcacata
     4501 gacgctggcc ggttgttgag acagggtgct attcagttcc ctgatagtac cgggcttcgt
     4561 attaaccgat acgcgatagg ggttaaacca ggcgtgtact ttcatcccac gcttgtgggc
     4621 ttcgtcgagc ataaattgca gcggatcgta acccggattt tcaccaatct taccggtcat
     4681 aagatcggac cacggcaaaa ttttcgatgg ccacagggcg gtaccatccg gcttgacctg
     4741 aaaaaagacc gtgtttatgc cgagacgttg cagatgatcc agtttgtcga tcatcgcctg
     4801 ttgttgtaca cgtgcccggc tggtggggtt actattgtta accgaggaaa ccggtggcca
     4861 gtcgaggcga gacaccgtgg ccagccagat gccacgcatc ggttgtgacg attgttgcgt
     4921 cgtggctggt ggctttgagc ccgctggtgg tgtcaccatg gactctggag gcgtgctttt
     4981 acaactacac agtaaaagtg ccagtgcaac cagtatcgct ggtcttctaa tcgctaattt
     5041 ctcgtttcgg gagcagatat ccatatgtgc tggtttccgg taaacagatg tgcgctcatt
     5101 ctcgtactta tcccggttaa gtcaatacga caacaagcac gaaaaaggga gcgatgaatt
     5161 atcgctccct tgtcttataa gcattcagac atggttagtg tttcttgtca ttcatcacaa
     5221 tatagtgtgg tgaacgtgca cgcgggtgca ggaagaagtg accttttggt gcgttctgac
     5281 tgttgattgg ctccagagtg acgccggtat tcgctttgct tttacgatca tgaacagcat
     5341 agagaataaa gggcagggca agtaccacca ggaaactaac gaccagtaat tcaacataca
     5401 tatcggtaga gtcaccctgg atgttatccg gcggcaggaa ggaaacaata aacgccataa
     5461 ttgaagtcag cagaccgaca atagccacga ccagtttcac ccctttacca ccagggatat
     5521 taaatgtgcg ttttaagtca ggatgtttaa gcaccaacac aatgtagcca ataaacagca
     5581 tgaaataagc acacagataa atcaccaccg tcagcgccag tgcgatcagg aaggacatgt
     5641 tgttaccgcc accggtattg gtgaggatga tcaacgcgat agaagtaatc accaactgcg
     5701 aaatgaccag cgttaccggt acgccatttt tgttcatttt agcgaatgcc gctggcagca
     5761 ggtttttctg cgctgtgaca tacatcccgc gagaaggacc aacaatccag gaagcgattt
     5821 ccgccagaac acccagcaac agcagtgcgg agatcacgcg aaccgtccac tcaatttccg
     5881 gtgccacatg tgacatcaga acggtaaagg tttgcattac ccctgcggag aggttgattt
     5941 cattacccgg aatgaccatc gcaatagaca aaccgccaac agagcttagg cagattgccg
     6001 ccaccatcag cagtaacata gccagtggat agtcgcgccc ggggttgctc atttcattga
     6061 cgtgggttgc ggaagcttca acgcccatat aactcaaaat gaaggcaaca aatacaacca
     6121 gggtgcccac tttagagaag tcagggaaga aggtcttcgc atccatttcg atagcaacgg
     6181 gggcaccgga gtgcagataa atagccgcta atgcgatcaa aataaatgca ggtaacagga
     6241 taccggcgaa gaagccaact ttagcaattc gcgccgtgta tttcgtgcca ccaaactgcg
     6301 ttaatgccag cgcccaaaga atgatgagtg ctgcaatagt tttggtaatg gggtcttcat
     6361 tcagcgctgg ccatttcagg atgtaggaga gtgcccctaa cacgaaatag agcatcggaa
     6421 taaaaccaat ggcgatttgc agatagccaa atgagatcgc tgcaaatccc catctcggcc
     6481 ccagagtatt tgatacccag gcgaagacgc caccttcttc ccagccgtcg acggtagcca
     6541 tttccgcagc acaaagtccc acgggaataa accataaaat cccgcctaat agcaggaaga
     6601 agactaatga aaagcccgat gttgcgaagg tagggtattc ataaacagcc attaccatcg
     6661 atgccgttat ggcaaaaaat ccgagtaatg tgagctgctt agctttacct gtctgtactg
     6721 atgtagccat attatccccc taaaacggta ttcctgtcgg aaccgcacct gttttgttct
     6781 gaagcgtatt cagaacaata ttttccgttg ctaatgccag tgaacagact ttggaaattg
     6841 tcccgaaacg gcttcgtttc gggacaccgt taccgttaaa cgttatcagg tgtgtttaaa
     6901 gctgttctgc tgggcaatac cctgcagttt cgggtggtcg ctgagatatt tcagggaggc
     6961 tttgtagtct tccagcaaca gttcagcaaa gtccatttcg aagccgcgac gacacataat
     7021 gcgcatcacc acgatgtcgg tggcttcacc gccgagagtg aaggccggaa cctgccagcc
     7081 gcgcagacgc agacgttcag agaggtcgta cagggtgtat cctggatctt caccatcttt
     7141 cagtttgaag caaaccgccg ggatgccttc gtccgggcga ccggtacaga tgaactcata
     7201 cggccccagt ttggc
//
LOCUS       DACADA010000205         7211 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.207, whole genome shotgun sequence.
ACCESSION   DACADA010000205 DACADA010000000
VERSION     DACADA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.207"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            156..1406
                     /gene="frc"
                     /locus_tag="ISB17_004307"
     CDS             156..1406
                     /gene="frc"
                     /locus_tag="ISB17_004307"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAP1982171.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1460..3154
                     /gene="oxc"
                     /locus_tag="ISB17_004308"
     CDS             1460..3154
                     /gene="oxc"
                     /locus_tag="ISB17_004308"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAP1982172.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3224..4168
                     /gene="yfdV"
                     /locus_tag="ISB17_004309"
     CDS             3224..4168
                     /gene="yfdV"
                     /locus_tag="ISB17_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAP1982173.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4242..5387
                     /gene="yfdE"
                     /locus_tag="ISB17_004310"
     CDS             4242..5387
                     /gene="yfdE"
                     /locus_tag="ISB17_004310"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAP1982174.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5443..>7211)
                     /gene="evgS"
                     /locus_tag="ISB17_004311"
     CDS             complement(5443..>7211)
                     /gene="evgS"
                     /locus_tag="ISB17_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP1982175.1"
                     /translation="TADYYKNAMLPLENSESPFKDVFSNTHEVTAETKENRKIYTQVF
                     EIDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVA
                     KSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEIL
                     DVDKIESGNYQLQPQWVDIPTLVQNTCHSFAAIAASKSIALSCSSTLPERYLVKIDPQ
                     AFKQVLSNLLSNALKFTTEGAVKITTSLGHIDDNHAVIKMTIMDSGSGLSQEEQQQLF
                     KRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTIFTITIPVEITQQVA
                     AVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKISMQ
                     HYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFK
                     PLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDL
                     PAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLL
                     NSVKEHIAELDQEITVFCQQND"
BASE COUNT         1892 a         1512 c         1710 g         2097 t
ORIGIN      
        1 ttggaagtag cgatgaaatg attttcagca cttgatacag gaatttaaaa atatcgagtg
       61 cagctacgac gatattttgc agattattta tagaggcgtt atgccttaaa taaacgctaa
      121 attaacacat atttaattaa ctaataggta ttgctatgtc aactccactt caaggaatta
      181 aagttctcga tttcaccggt gtgcaatctg gcccatcttg tactcaaatg ctggcctggt
      241 ttggcgctga cgtcattaaa attgaacgtc ccggcgttgg tgacgtaacg cgccaccagc
      301 tgcgagatat tcctgatatc gatgcgcttt acttcaccat gcttaacagt aacaaacgtt
      361 ctattgaatt aaataccaaa acagcggaag gcaaagaggt aatggaaaag ctgatccgcg
      421 aagctgatat cttagtcgag aactttcatc caggggccat tgatcatatg ggcttcacct
      481 gggagcatat tcaagaaatc aatccacgtc tgatttttgg ttcgatcaaa gggtttgatg
      541 agtgttcgcc ttatgtgaat gtaaaagcct atgaaaacgt tgctcaggca gcgggtggcg
      601 cggcatctac tacaggtttt tgggacggtc cgccgctggt aagcgctgca gcgttgggtg
      661 acagcaacac cggaatgcat ttgctgatcg gtttacttgc tgctttgctg catcgcgaaa
      721 aaacggggcg tgggcaacga gtgaccatgt caatgcagga tgccgtgttg aacctttgcc
      781 gcgtgaaatt acgcgaccag cagcgtctcg ataaattggg ttatctggaa gaatacccgc
      841 agtatccgaa tggcacattt ggtgatgcag ttccccgcgg tggtaatgcg ggtggtggtg
      901 gtcagcctgg ctggatcctg aaatgtaaag gctgggaaac cgatcctaac gcctatattt
      961 atttcactat tcaggagcaa aactgggaaa acacctgtaa agccatcggc aaaccagaat
     1021 ggattaccga tccggcatac agtacagccc atgcccgaca gccacatatt ttcgatattt
     1081 ttgctgaaat cgaaaaatac actgtcacta ttgataaaca tgaagctgtg gcctatttga
     1141 ctcagtttga tattccttgc gcaccggttt taagtatgaa agaaatttca cttgatccct
     1201 ctttgcgcca aagtggcagt gttgtcgaag tggaacaacc gttgcgtgga aaatatctga
     1261 cagttggttg tccaatgaaa ttctctgcct ttacgccgga tattaaagct gcgccgctat
     1321 taggtgaaca taccgctgct gtattgcagg agctgggtta tagcgacgat gaaattgctg
     1381 caatgaagca aaaccacgcc atctgatatt aacgggggct tctggctccc aatttataaa
     1441 aatttcgagg ttattaatca tgtcagatca acttcaaatg acagatggta tgcatatcat
     1501 cgttgaagca ttaaaacaga ataatattga cactatttat ggtgttgtag gtattcctgt
     1561 gacggatatg gcgcgccatg cccaggcgga aggcattcgt tatattggtt ttcgtcatga
     1621 gcagtcggca ggttatgccg ctgcagcaag cggttttctt acccaaaaac cagggatctg
     1681 cctgacagtg tctgcgccag gattcctcaa cggtttgacc gcattggcca atgcaacggt
     1741 aaatggtttt ccgatgatca tgattagcgg ctccagcgac cgcgcgatcg tcgatctcca
     1801 gcaaggtgat tatgaagagc tggaccaaat gaatgcggca aaaccgtatg ccaaagcagc
     1861 atttcgcgtt aatcagccgc aggatcttgg cattgcattg gcacgcgcta tccgggtctc
     1921 agtatcgggt cgtcctggcg gtgtttatct tgatttgcca gcaaatgtcc tggccgcgac
     1981 gatggaaaaa gacgaagcgt taaccacgat tgttaaagtt gaaaatccgt cgccagcatt
     2041 attgccatgc ccgaagtcag tcactagcgc aatttcgctt ttagcaaaag ctgaacgacc
     2101 attaattatt cttggcaaag gcgcggcgta ttcacaatct gatgaacagc ttcgtgaatt
     2161 tattgaaagt gcccagattc cattcctgcc aatgtctatg gcgaaaggga tcctcgaaga
     2221 tacgcatcca ctctctgcgg cagctgcgcg ttcgtttgcc ctggcaaatg ctgacgttgt
     2281 catgcttgtt ggtgcacgac tgaattggtt actggcacac ggtaaaaaag gatgggcggc
     2341 agatacacag tttattcaac tggatattga accgcaggaa attgacagca accgccccat
     2401 tgctgtgcca gtcgttggcg atattgcatc cagtatgcaa ggtatgctgg cagaattgaa
     2461 acaaaacaca tttacgactc cactggtgtg gcgtgatatt ttaaatatcc acaagcagca
     2521 aaatgcacaa aaaatgcatg aaaagttaag tacagatacc caaccattaa attactttaa
     2581 tgcattgagt gccgtgcgcg atgttttgcg cgagaaccag gatatttatt tagttaatga
     2641 aggggcaaat accctggata atgcacgaaa tattattgat atgtataaac cacgtcgtcg
     2701 tctggattgt ggcacctggg gtgtcatggg catcggtatg ggctatgcca tcggtgctag
     2761 cgtgacctct ggttctccgg ttgtcgctat tgaaggtgat agtgcttttg gtttcagtgg
     2821 gatggagatt gaaacgattt gtcgatataa cctgccggtg acgatcgtta tttttaataa
     2881 tggcggcatc tacagaggag acggtgttga cctcagcggc gctggtgcac catcgccaac
     2941 ggatctgttg caccatgcaa ggtatgacaa attaatggat gcgtttcgtg gcgttggcta
     3001 taacgtcacc acgacagatg aacttcgtca tgctttaacc accggaattc agtcgcgcaa
     3061 accgaccatt attaatgtgg tcatcgaccc agcagcagga actgaaagtg gccatattac
     3121 caaacttaac ccaaaacaag tcgctggtaa ttaaatactg acatcgtctc ctcttgccaa
     3181 cttattatgc gatggtgagg ggacccttat cggaaatact attatgctaa cattttttat
     3241 tggcgattta ttgcctatta tcgtaatcat gctgttgggt tattttagcg gcagacgaga
     3301 aactttttca gaagatcaag cccgggcatt taataaactg gtattaaact acgctcttcc
     3361 tgcggctcta tttgtttcta ttaccagggc aaacagggaa atgatttttg cggacactcg
     3421 cctgaccctt gtatcacttg tggttattgt cggatgtttc tttttctcct ggttcggttg
     3481 ctacaaattt tttaaacgta cccatgcaga agcagcagta tgtgcattaa ttgcaggttc
     3541 acctaccatt ggattcctgg ggtttgcagt tctcgatcct atttatggtg attccgtatc
     3601 aacaggttta gtggtagcaa ttatttctat tattgttaac gcaattacta ttcctattgg
     3661 tctgtatttg ctgaatcctt cctcaggagc ggatggtaag aagaatagta atctgagcgc
     3721 attaatttct gcggcaaagg agccagtagt atgggcacct gttctggcaa cgatcctggt
     3781 gctggttggg gtaaaaattc cggcagcatg ggacccaacc tttaatctga ttgcgaaggc
     3841 taactcaggg gtagcggtat tcgctgcggg gttgactctg gctgcacata aattcgagtt
     3901 cagtgccgaa attgcttata acactttcct gaagctgatt ctgatgccac tggcgctgct
     3961 tctcgtcggt atggcatgtc atttgaatag cgaacatttg cagatgatgg tactggcagg
     4021 cgcattaccg ccggcattct ccggaatcat tattgccagc cggtttaatg tctacacccg
     4081 tactggtaca gcgtcattgg ctgtgagcgt actgggtttt gtcgtcacgg ctcctttgtg
     4141 gatttatgtc agtcgactgg tttcataatt tatcatgtta ttgattttac ataaatgccg
     4201 aagtgtcttt tcaccttcgg cttttttcaa ggcataagca aatgacaaat aatgaatgca
     4261 aagggccgtt tgaaggctta ttagttatcg atatgacgca tgtccttaat gggcctttcg
     4321 gaactcaact tctttgtaat atgggcgcaa gggtaattaa agttgagcca ccgggtcatg
     4381 gtgatgatac ccgcacattt ggtccctatg tggatggaca gtcactctat tacagtttta
     4441 ttaatcatgg caaagagagt gtggttcttg atttaaagaa tgatcacgat aaaagtatat
     4501 ttataaatat gcttaaacaa gctgatgtat tagctgagaa ttttcgccca ggtacaatgg
     4561 aaaaactcgg gttttcatgg gaaaggttac aagaaatcaa cccgcgcctt atatatgctt
     4621 catcgtcagg tttcggacat accggtccgc taaaagatgc tcctgcctac gataccatca
     4681 ttcaggcaat gagcgggata atgatggaaa caggataccc tgatgctccg ccagtgcgcg
     4741 ttggtacctc tcttgcggat ctctgtggcg gtgtttattt attcagtgga atagtgagtg
     4801 cactttatgg ccgcgaaaag agccagagag gggcgcatgt cgatatagcg atgtttgatg
     4861 ccactctgag ttttctggag catggactga tggcatatat cgcgactggg aagtcaccac
     4921 aacgcctggg aaatcgccat ccctacatgg caccttttga tgttttcgat actcaggata
     4981 agccgattac gatttgttgt ggtaatgaca agcttttttc tgcgttatgc caggcactgg
     5041 agcttacgga actggttaat gatccccgat ttagcagcaa tattttacgc gtacataacc
     5101 aggctattct taaagaatat attgagcgga cgttaaaaac gcaggtagct gaagtttggt
     5161 tagccagaat acatgaagtt ggtgtgcccg tcgcgccgtt attaagtgtg gctgaggcca
     5221 ttaatttacc acaaactcag gcgagaaata tgttgattga agccggggga ataatgatgc
     5281 cgggtaatcc gataaaaatc agcggctgcg cggacccgca tgttatgccg ggagcggcaa
     5341 cgctcgacca gcatggggaa caaattcgcc aggagttctc atcataaatg taagatgtgc
     5401 tggtaaatag ctcccacatt tgaacattgt gggagccgct atttagtcat tttgctgaca
     5461 gaaaacagta atctcctggt ccagctctgc aatgtgttct tttacagagt tcagcaactg
     5521 aagaatttca ggcttactgt catctgaaac aggtgttatt tctaactggt ggctaatatt
     5581 aatcaacttt tgcaaattca ggatgttagc cgcaccgtgg atgcgatgaa tacactgatg
     5641 gaaagttctg ttatcgccag cttctagtgc atgaaacgca gcgggtagat ctttatgcgt
     5701 ttcatgctgg aaagtcatga gaatctcctg catcagttgt agatcattcg ccgtattatt
     5761 cttcagggcc tcgatatcaa ggtggcgata ctgaggtgca atatgcgcaa cctggtgtaa
     5821 ctgacttaaa tgtgttttca gtacatccag agtcaacggt ttgaacaaac ataagttcat
     5881 gccgcaattt aacccttttt cacgttcgtt agcctgtgcg ttggctgtaa gcccccagat
     5941 gggtaaggaa gaattttgct cacggagttt gcgagtcaac tcaaaaccat ccatattcgg
     6001 catattaacg tcagtaataa gcagatcata atgttgcata ctgattttgt gtagcgcttg
     6061 cacaccatca gtggcttcat caacatcata tcctaataga tttagctggc gtttgagtaa
     6121 tagcctgttg gtcggatgat catccgcgat taatatgctc aacttttcag gtagtgttat
     6181 aggttgttct gcttttgcct cgacagccgc cacttgttgg gtaatttcta ccgggattgt
     6241 gatcgtaaat atcgttccta tgcctggatg actttctaat gacaaatcgc cctgcatgtt
     6301 tttaattaat tctttgcaga tcattaagcc taaaccagaa cctgtttgct gacgacctgc
     6361 acttgtttgg ctatagcgtt taaacagttg ttgttgttct tcctgcgata atccacttcc
     6421 agaatccata atcgtcattt tgataacagc gtggttgtca tcaatgtgac ccagggaggt
     6481 cgtaattttt actgccccct cggtagtaaa tttgagagca ttactcagta aattagataa
     6541 gacctgctta aacgcctgag ggtcgatctt aaccaggtag cgttcaggaa gcgtactgct
     6601 gcaacttaat gcgatcgatt tgcttgcagc aatcgcagcg aaagagtgac aagtgttctg
     6661 gactaaagta gggatatcga cccattgtgg ttgaagttga tagttacccg attcaatttt
     6721 gtcgacatca aggatttcac caattaagcc gaggagtgat tgtccggtgg cgtaggcaag
     6781 tgaaatcgcc tccacccgtt gttccttgct aagaccagaa cccgacagaa gttccaggaa
     6841 gcccataatg gagcttattg gtgttcttat ttcgtgactc attgttgcca gaaactggct
     6901 ttttgcgaca gttgcattga tcgctttatt tctctctact tcgagtgcat gaattaaatc
     6961 acgcgtctcg gtaatatctt gccaaccaca aatataaaca gcatgttcgc ttgctggcag
     7021 attacataag gtatgccagt gattaatgca tcttttctcg atgccattat caatttcaaa
     7081 tacctgtgtg tatatttttc ggttttcttt cgtttctgct gtgacttcat gcgtattaga
     7141 aaaaacatct ttaaaggggg attcactgtt ttctaatggt aacattgcat ttttatagta
     7201 atcagcagtg a
//
LOCUS       DACADA010000206         7160 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.208, whole genome shotgun sequence.
ACCESSION   DACADA010000206 DACADA010000000
VERSION     DACADA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.208"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            401..1315
                     /locus_tag="ISB17_004312"
     CDS             401..1315
                     /locus_tag="ISB17_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HAP1982176.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1335..2672
                     /locus_tag="ISB17_004313"
     CDS             1335..2672
                     /locus_tag="ISB17_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HAP1982177.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLVQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDVCAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2685..3179
                     /gene="hutX"
                     /locus_tag="ISB17_004314"
     CDS             2685..3179
                     /gene="hutX"
                     /locus_tag="ISB17_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HAP1982178.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            3179..3802
                     /gene="chuY"
                     /locus_tag="ISB17_004315"
     CDS             3179..3802
                     /gene="chuY"
                     /locus_tag="ISB17_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HAP1982179.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3887..4843
                     /locus_tag="ISB17_004316"
     CDS             3887..4843
                     /locus_tag="ISB17_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1982180.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4840..5610
                     /locus_tag="ISB17_004317"
     CDS             4840..5610
                     /locus_tag="ISB17_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HAP1982181.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5662..6309)
                     /locus_tag="ISB17_004318"
     CDS             complement(5662..6309)
                     /locus_tag="ISB17_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP1982182.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6373..6699)
                     /gene="hdeB"
                     /locus_tag="ISB17_004319"
     CDS             complement(6373..6699)
                     /gene="hdeB"
                     /locus_tag="ISB17_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAP1982183.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6815..7147)
                     /gene="hdeA"
                     /locus_tag="ISB17_004320"
     CDS             complement(6815..7147)
                     /gene="hdeA"
                     /locus_tag="ISB17_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAP1982184.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1800 a         1799 c         1727 g         1834 t
ORIGIN      
        1 caaacgtgat aatgagaatt attatcgatc aattttttat gatcaagctt tttttaaccg
       61 ccattaacac aataaacagg gggttatgaa atctatccag catccagaaa accatcctgt
      121 gtttatggct atccccccac aacctcatca caaaacgttg tcgttaacag tgataatttt
      181 ctcttctctt tgctctgcaa gccgcgatat ctccgggttc tcagcttttt cccgcgctga
      241 aatttaatcg gtaacaagaa cattagctga cgaagcatct tgataaaaat caaaaataaa
      301 tacgcttgta acgccgtgcg tattgaatcg acggttgtat ttcatatata actcaaatga
      361 taatcatgat cattctcaat ttgataagag ttctcacatt atgccaagga tcatcaccag
      421 gccgtttctt ttttccccac taacgttatg tatttcagcc gtggtctctg ccgcgaagac
      481 catggtaaaa cgaaaaaaat tgtttacggc attactggct cttagctgga cttttagcgt
      541 aacggctgcc gaacgtatcg tggtcgcagg aggatcgctg acggagctaa tctacgcgat
      601 gggcgctggc aaacgcgtgg ttggtgtcga tgaaacgaca tcctatccac cagaaaccgc
      661 caaactgccg catattggtt actggaaaca actcagcagc gaaggcattt tgtcacttcg
      721 cccggatagc gtaattacct ggcaggatgc aggaccgcaa attgtgctcg accaactgcg
      781 ggcgcaaaaa gtcaacgtcg tcactctgcc gcgtgtacca gccacccttg agcagatgta
      841 cgccaacatt cgccaactgg caaaaacgtt acaggttcct gaacaaggcg aggcgctggt
      901 gacacagatc aaccaacgcc tggagcgagt acagcaaaac gtggcggcca aaaaagcccc
      961 ggttaaagcc atgtttattc tctccgcagg cggaagtgcg ccacaggttg ccggtaaagg
     1021 cagcgtcgcg gacgccattc tttcgcttgc cggagcagaa aacgtcgcta cccaccagca
     1081 gtacaaaagc tacagcgcgg aatcgctgat tgcggctaac cctgaagtga ttgtcgtcac
     1141 ctcacaaatg gtcgatggcg atattaatcg gctacgttct attgccggaa ttacccacac
     1201 tgccgcatgg aaaaaccagc gcattatcac cgttgatcaa aacctgattc tgggaatggg
     1261 tccgcgtatt gctgatgtcg ttgagtcttt acaccagcag ctttggccgc aataaatgga
     1321 tttaaatcag acgaatgaac acaaataaca ccctggatct cacgccacat tttgctttag
     1381 acggtgatca gccctttaaa gacaggcgcg ccatgatgcc gtttcgtggg gccattccgg
     1441 ttgccaaaga gcaactggta cagacctggc aagagatgat caatcaaacg gtatcaccac
     1501 gtaagcgact ggtttaccta cacattcctt tttgtgcgac acactgcaca ttttgtggtt
     1561 tttatcagaa ccgttttaat gaagatgtgt gtgctcatta taccgacgcc ctgattcgtg
     1621 aaatagaact ggaagcagac agcgtattgc atcagtcggg ccccatccac gcggtctatt
     1681 tcggtggcgg tacgccttcc gcgctttcag cacacgattt ggccagaatc atcaccacat
     1741 tacgagaaaa gttgccactg gcccctgatt gtgaaatcac tatcgaaggc cgggtactga
     1801 attttgacgc tgagcgaatc gatgcttgcc ttgatgctgg agcaaaccgc ttctcaattg
     1861 gtattcagtc gtttaacagc aaaatccgca agaaaatggc ccgcacctcg gatggcccaa
     1921 ccgctattgc gtttatggaa agcctggtca aacgcgaccg tgccgcggtg gtctgtgacc
     1981 tgctgtttgg tctgccgggt caggatgcgc aaacctgggg agaagatctg gctattgccc
     2041 gcgatatcgg tctcgacggc gtcgatctct atgcgctcaa tgtcctgccc aatacaccgc
     2101 tgggcaaagc cgtggaaaat gggcgtacta ccgtgccctc tccggcagaa cgtcgcgatc
     2161 tttacctgca agggtgtgat tttatggacg atgccggttg gcgctgcatc agtaacagcc
     2221 actggggccg taccacacgc gaacgcaatc tctataacct gctgattaaa caaggtgccg
     2281 attgtctggc ctttggttcc ggagccggcg gttcgattaa tggttactcc tggatgaacg
     2341 aacgcaatct tcagacctgg catgaatccg tcgcagcagg caaaaaaccg ctgatgatga
     2401 tcatgcgtaa cgccgaacgt aacgcgcagt ggcgtcatac cttgcagtcg ggcattgaaa
     2461 cggcgcgtgt accgctggac gaacttacgc cacatgccga aaaactcgcg ccgttactgg
     2521 cccagtggca ccaaaaaggc ttaagccgcg atgcgtcaac gtgtctgcgg ttgaccaatg
     2581 aaggtcgttt ctgggcaagc aacattttgc agtctcttaa tgaactgatt caggtactta
     2641 atgcgccagc aattgcgctt gaaaaaccat aacaggagtg atttatgagc catgtctcgt
     2701 tacaggaatt tttgaaaacg gagccagacg gtacgctgga ggcgatcgcc gaacaataca
     2761 acaccacgct gctggaggta gtgaaaaatc tgccctctcc gactgttgtg tcaggtgata
     2821 aattcgatac tgtctgggat accgtctgcg aatggggcaa cgtcaccacg ttagtacata
     2881 ctaccgatgt aatactcgaa tttagcggcg aactgccttc gggtttccat cgccacggct
     2941 atttcaacct acgcggaaag cacggcatgt ccgggcatat caaagcagaa aactgcacgc
     3001 atattgcctt gatcgaacgt aaatttatgg gcatggacac cgcctctatt ctctttttca
     3061 ataaggaagg cagcgcaatg ctgaaaatct tcctcggtag ggatgatcat cgtcagctcc
     3121 tgagcgaaca agtcagcgcc ttccatgctc tggcagcatc gttaaaggaa cacgcctgat
     3181 gacaccgtgg ctactctttg gtgcgggcgg aaaaggtgtg ggtgccagaa cgcttgaact
     3241 ggcgctggcg gaacaacgtc cggtcgttgc tgtcattcgt catgccgatg ccgccacgaa
     3301 actggcgcaa caaggcgtac aggtttttac aggcgacgcc tgtgatgcca gcgtagtcgc
     3361 ggctgcttgc cgcgcagctg gcccggatgc gcttatcatc tcaacaatgg gcggagctca
     3421 ggattatctg gcgcacagaa cagtgattga tgaggccgag aaggctggca tcagtcgcat
     3481 gattctggtg acctctttgg gatgcggcga tagctggccg tttttatcag agcgggcaaa
     3541 ggctgcgttt ggtcaggcgg tacgggaaaa aacgctggcg gaaagctggt tgcaaaccag
     3601 ccagcttgat tatgccattc tccgccctgg cggtctgctt gatggtgcgg caacgggcaa
     3661 agcacaacgg atacaaaatc aggagtgtca tggttttgtt aaccgtgctg atgtcgccgc
     3721 acatatccat gaactggcaa atgcgccagc acttaaccag caggtctaca gtctgattga
     3781 accggacctg aaaccggcgt gatcccccgg cgcggcatgt ccgcgccgta atgtcgtaac
     3841 ggagttttgc gtgctcaagg attcactctc ttccgccagg gtctttatgg ggctatcact
     3901 attattgcta gctctggtgc tgtttggtgc cagtcaggga gcgttaaaga tcagttttga
     3961 tgcccttttt gatgaggaat accgcgatat ctggctcaat attcgtctac cacgggtttt
     4021 gctggcggtg ctggtaggtg cagcgttggc aaccgcaggg gtaattatgc aagggctttt
     4081 tcgcaaccca atggctgacc ctggattact tggcgtcagt agcggttctg cattaatggt
     4141 tggcgttgct attgtgctgc ccttctcctt cccagtggtg ctggtgctct atgagcaaat
     4201 ggtgtttgcc attgccggaa gtttagtggt ctgcaccatc atttttctca tcacgcagcg
     4261 ccatcgcgat ggcagcatga tgcaattatt actcgccggt atcgccatca atgccctgtg
     4321 cggcgcagcg atcggcatcc tgagctatat cggcgatgag cagcagctac gacaactcac
     4381 attgtggatg atgggcaatc ttggacaggc gcaatggccg acgttattgg ttgccagttc
     4441 attcatccta ccggccatta tcgcaacaac ttgtctcgcc ggaacgctga atttactgca
     4501 gctcggtgat gaagaagccc actacctcgg cgtgaacgtt aagcgtaaac gccagcaatt
     4561 actgttagtg agctcactgc tcgttggtgc tgccgtatcg gtaagcggta ttatcggctt
     4621 tattggcctg gtgatcccgc atctgattcg catgactacc ggggcaaatc accgctggct
     4681 aatcccttgt tccgccctcg ccggagcctg tttattgctg atggcagaca cgcttgcccg
     4741 cacgctggta cagccagcag aaatgcccgt gggattatta accagcctgc ttggtggccc
     4801 ttattttatg tggttgattc tgcgcaaccg gaggatcaca tgatcagcgc ccagaatctg
     4861 gtttatagcc ttcaggggcg gcgtctgact gataatgtct cgctgacttt tcccggtggg
     4921 gaaattgttg ccatcctcgg ccctaatggt gcgggaaaat ctacgctgct tcgccagcta
     4981 accggctatc ttcaacctga tagcggcgaa tgtcggttgt ttaataagcc attaaatgaa
     5041 tggtcgatta ctgaactggc gaaacaccgg gccgtgatgc gtcaaaatag ccacatggcg
     5101 tttccattca gcgtacaaga agttatccag atggggcggc atccgcatcg tactggtaat
     5161 caggataatg aaacagcaca aataatggcg ttatgtgact gtcaggcgtt agcaaaccgc
     5221 gactatcggc aattatccgg tggtgaacaa cagcgcgtgc aattagcgcg cctgctggtg
     5281 caactctggg aacctactcc ctcaccgaaa tggttatttc ttgatgaacc aacttcggcg
     5341 ctggatattc accatcagca gcacttgttt cgtttattac gccagttagt tcacgaacgg
     5401 cagttcaatg tttgctgcgt attgcacgat ctaaatcttg ccgcgcgtta tgcagaccgt
     5461 gttgtattaa tgcaaaaagg aaaggttatc gcaaatggta agccgcagga cgtattgaca
     5521 cagcaggcgc tgacaatgct ctacggcgcg gatattaccg tacttaaaga tcctgccaac
     5581 cattcaccac tgatcgtcct cgatcattaa aaccaacagg agtaccgcac tggtactcct
     5641 gcgaataaat aactatacag attagctaat tgaaacgcct ttcacccctg ccataccttt
     5701 taataatcgc aacagatctt ctatcgacgt cgtggcatga agctggatat caatggcgac
     5761 cacttcatga tcttcatttt cctgcaacga aactaaatcc gtttttattt tttgctgctt
     5821 aaaccagtcg agcattgata ccgtattacc gttcactaat gtcagctgta aatggtaatt
     5881 tttgttcatc aggcggaagg ttaattgatg gaagacttcc agcaccagca aggtcatcac
     5941 tgaaccataa atacccagtt cgtacatacc gctgccaata accataccaa tggcggcggt
     6001 cacccagata tccgctgccg tcgtcagacc tacaatattt tggttacgaa cgagaatgtt
     6061 acctgcaccg ataaacccga cgcccgtcac aacctgagca gcaatacggc tggggtctag
     6121 tccgacgtga cctaaagaca gcacgtcagc aaaaccatat ttcgaaacaa tcataaacag
     6181 ggcgcttccc atgccaatta atacatgtgt gcgtaaccct gctcctttgc cgcgcatttg
     6241 tctttccatg ccaatagcgc cacaggcaat taccgcaagt ataagtcgta taataaattc
     6301 tgctgtcata taaaaatgca aaaaggagca gcaagatggc tcaacttgct actccttttt
     6361 acttgtacct cattaattcg gcaagtcatt agatgcttga tttttgaagg tatacaaatt
     6421 tttctgcggg tttttcttgc agtattcgat cactttagga atttgagtga gatcggtttc
     6481 atttaaagta acggtatcgc cacctttata tactgtttct tcatgcagca tccaccacgc
     6541 aactggtgtc attgcttttg gattcagatc aataaattcc tggcaggtca tatctttagc
     6601 ggattcattg gctgccagcg cagattgtgc gttcaccagt gacaaagccg ctacagcgcc
     6661 cataaaaata aacgctttac ggagagatga aatgttcatt ttgtaaccca ttcaatatag
     6721 agattatata caaacaggag gaatagctgc ttttaaagaa gatattccgg cctgttcaat
     6781 atgcaaggaa atacgatgta acgcattccc ggaattacat atctttctta attttgtccc
     6841 attcgccttt aactttatct ttaaagctgg cttttttatc ctgagtacaa gcctgaacga
     6901 tagctggggt tacggttgca ataccctgaa catctaaaac cgcatcttct ggtttatctt
     6961 tgttgttcag tgcttcagca aaaccaactg cagttggctg gaaggattcg tccacagcca
     7021 ggaaatcttc acaggtccag gagttgaccg gttttttgtt atcagctgct ttttgtgcat
     7081 ccgctgcatt gctcactact ggcagaagaa gcagaccacc aagaataacg cctaatactt
     7141 ttttcatcgt aatatcctca
//
LOCUS       DACADA010000207         7152 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.209, whole genome shotgun sequence.
ACCESSION   DACADA010000207 DACADA010000000
VERSION     DACADA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.209"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..325
                     /locus_tag="ISB17_004321"
     CDS             <1..325
                     /locus_tag="ISB17_004321"
                     /inference="COORDINATES: protein motif:HMM:NF016200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAP1982185.1"
                     /translation="TFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLC
                     LGLAWGAIEKTAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSR
                     NQQGA"
     gene            130..1605
                     /gene="yiaN"
                     /locus_tag="ISB17_004322"
     CDS             130..1605
                     /gene="yiaN"
                     /locus_tag="ISB17_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HAP1982186.1"
                     /translation="MSGAGLGRYRKNSARLEQLIAHSWRPRWLNVRRRYPHQSDYRIA
                     GIASLIPAVHKYPQPKPTRSLTMTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMD
                     IFDVQILAQTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLG
                     YVGVLAAMIMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPP
                     SIPLIIFGVSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVW
                     QSLVSGIWALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVL
                     INAAKTTSVVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVI
                     STVMDLTPTVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGV
                     AKLKFDDAVKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1618..2604
                     /gene="yiaO"
                     /locus_tag="ISB17_004323"
     CDS             1618..2604
                     /gene="yiaO"
                     /locus_tag="ISB17_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HAP1982187.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2608..4104
                     /gene="lyxK"
                     /locus_tag="ISB17_004324"
     CDS             2608..4104
                     /gene="lyxK"
                     /locus_tag="ISB17_004324"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HAP1982188.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4101..4763
                     /gene="ulaD"
                     /locus_tag="ISB17_004325"
     CDS             4101..4763
                     /gene="ulaD"
                     /locus_tag="ISB17_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP1982189.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            4756..5616
                     /locus_tag="ISB17_004326"
     CDS             4756..5616
                     /locus_tag="ISB17_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP1982190.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMLETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5610..6305
                     /gene="araD"
                     /locus_tag="ISB17_004327"
     CDS             5610..6305
                     /gene="araD"
                     /locus_tag="ISB17_004327"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1982191.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6337..7152)
                     /locus_tag="ISB17_004328"
     CDS             complement(6337..7152)
                     /locus_tag="ISB17_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1982192.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         1624 a         1923 c         1934 g         1671 t
ORIGIN      
        1 aacctttatt ggcgcaatcg ttgcttatat ggacaacagc catgtgcagg ttactttttt
       61 agtcgaaaaa ctctcaccgg ctaatcaaca acgtgtttcg ctgctaaccc atacgctcat
      121 tttattgctg tgtctggggc tggcctgggg cgctatagaa aaaacagcgc aagactggag
      181 caacttatcg cccattcttg gcgtccccgt tggcttaatg tacgccgccg ctatccccac
      241 cagtctgatt atcgcattgc tggaattgcg tcacttatac cggcagttca caaatacccc
      301 cagccgaaac caacaaggag cctgacgatg acaatcctta tatttattgt cagtttgtta
      361 ggggctatcg caattggtgt tcccatcgcc tgggcattat tgttgtgcgg catctcattg
      421 atgttctgga tggatatctt tgatgtgcaa attctggcac aaacgttggt aaatggcgca
      481 gacagcttct ccctgttagc catccccttc tttgtactgg cgggtgagat catgaacgcg
      541 ggcggcctgt cgcaacgtat cgtcgacttg ccaatgaaac tggtaggcca caggccaggc
      601 ggtctgggtt acgtgggcgt gctggcagcg atgatcatgg caagcctgtc cggttctgcg
      661 gttgctgata ccgcagctgt cgcggcgctg ctggtgccga tgatgcgcca ggccaactat
      721 ccggtaaacc gcgccgccgg gcttatcggt tccggcggga ttattgcggc cattatccca
      781 ccttccatac cactcattat cttcggcgtc tccagcggac tctccatcag taagctgttt
      841 atggcgggca tcgcaccggg catcatgatg ggcgtcacgc tgatggttac ctggtggtgg
      901 caggcaaaac gactgaattt accttgtcag ccgaaagcct cactgcgaga agtctggcaa
      961 tccttagttt ccggtatttg ggcgctcttt ttaccgataa ttattattgg cggtttccgt
     1021 tcggggttat ttacaccgac cgaagcaggc gcggttgccg ccttctacgc tctgttcgtc
     1081 tcggtggtgg tctatcgcga gatgaccttc tccacgcttt atcatgtgct gattaacgcg
     1141 gcaaaaacca catccgtggt gatgttcctg gtggcctctg ctgcggtatc tgcatggctt
     1201 attacgatcg ccgaattacc catgatggta tcggagctat tacaaccgtt ggttgattcg
     1261 cctcgcttat tattcatcgt cattatgctc gctattatgg tgatcagtac ggtcatggac
     1321 ttaacgccta ccgtattaat tcttacacca gtattaatgc cattagtgaa agaagcggga
     1381 atagatccgg tctatttcgg catcatgttt attatcaact gttcaatatc actgattacg
     1441 cctccagtcg gtaatgttct taacgtggta tgcggcgtgg cgaaacttaa atttgacgat
     1501 gcggtaaaag gcgttgcgcc ttacgtcatg gttttattca tgttgttagc attatttatt
     1561 tttataccgg agctcattac cgctcctctt aaatggatga gttaaaagga aatcataatg
     1621 aaattacgtt ctgtaaccta cgctttatta attgccggag tggcgacgtt cagcacatct
     1681 tctctggcgg cacaatcatt acgtttcggt tatgaaacct cacaaaccga ttcacaacat
     1741 attgcggcga aaaaatttaa tgaattactg caggaaaaaa ccaaaggcga actgaaatta
     1801 aaactgttcc cggatagcac ccttggtaat gcgcaggcga tgatcagcgg cgtgcgtggc
     1861 ggcactattg atatggagat gtctggctct aacaacttta cgggactctc gcccgtcatc
     1921 aacctgctcg atgtcccgtt cctgttccgc gataccgctc acgcgcataa aacgctcgac
     1981 ggcaaagtcg gtgatgacct gaaagtctcc cttgaaggta aagggctgaa agtgctggct
     2041 tactgggaaa acggctggcg cgatgtcacc aactcacgcc agccagttaa aacacctgcc
     2101 gacctgaaag gactaaaaat ccgcaccaac aacagcccga tgaatatcgc cgcatttaaa
     2161 gtttttggcg ctaacccgat cccgatgccg tttgccgaag tctataccgg gctggaaacc
     2221 cgcactatcg acgctcagga acacccgatc aacgtcgtct ggtcagcaaa atttttcgaa
     2281 gtgcagaagt acctttctct gacccatcac gcctattccc cacttctggt ggtgatcaac
     2341 aaagcgaagt ttgatggctt aaccccggag ttccagcagg cactgatctc atctgcgcag
     2401 gaagcgggta gctatcagcg caaactggtc gccgaagatc agcaaaaaat catcgacggt
     2461 atgaaagaag cgggcgtgga agtcatcacc gatctcgacc gcaaagcctt tagcgatgcg
     2521 ctgggtaccc aggtccgtga catgtttgtt aaagatgttc cccagggtgc tgatctgctg
     2581 aaagccgtgg atgaggtgca ataaaccatg acgcaatact ggctggggtt agattgtggc
     2641 ggtagctggc tgaaagccgg gctgtatgac cgagaaggcc gggaggcggg cgtgcagcgc
     2701 ctgccgctgt gcgcattaag tccacagcca ggctgggcag agcgcgacat ggcagaactg
     2761 tggcaatgtt gcactgctgt cattcgctcc ctgcttaccc attctggcgt cagcggggaa
     2821 caaattgtcg gtatcggcat ctccgcgcag ggaaagggct tatttctact tgataaaaac
     2881 gacaaaccgc tcggtaacgc cattttgtcc tcggatcgcc gggcgatgga aatcgttcgt
     2941 cgctggcagg aagatggcat cccggaaaaa ctctatccgc tgacccgaca aaccttatgg
     3001 accgggcatc cggtgtcgct gttacgctgg ctgaaagagc acgaaccaga acgctacgcg
     3061 caaattggct gcgtgatgat gacgcacgac tacctgcgct ggtgtttaac cggcgtcaaa
     3121 ggttgcgaag agagcaatat ttctgagtcc aatctctaca acatgagtcg cggggaatat
     3181 gacccgtgcc taaccgactg gctggggatc gctgaaatca atcacgccct gccgcctgtt
     3241 gtcggatctg ccgaaatctg cggggaaatc accgctcaga cagccgtact gactggtctg
     3301 aaagcgggta cccccgttgt cggcggcctg tttgatgtgg tttccaccgc actctgcgcc
     3361 gggctcgaag acgaattcac cctcaatgcg gtgatgggga cctgggcggt gaccagcggc
     3421 ataacccacg gtttacgtga cggtgaagcg catccgtatg tctatggtcg ctacgttaac
     3481 gatggtcaat ttatcgttca cgaagccagc cccacctctt ccggcaatct cgaatggttt
     3541 accgcacagt ggggagaaat ctcatttgct gagattaatc aggccgtagc cagtttgccg
     3601 aaagccggtg gcgctctctt tttcctgccg ttcctgtacg gcagcaacgc cggactcgaa
     3661 atgaccagcg gtttctacgg gatgcaggcc attcacaccc gtgcacacct cttacaggct
     3721 atctatgaag gggtggtgtt cagccatatg acccatctca accggatgcg cgaacgtttt
     3781 actgatgtgc acaccctgcg cgtcactggc ggcccggcgc actccgatgt ctggatgcaa
     3841 atgctggctg acgtcagcgg tctgcgtatc gagctgccgc aggtggaaga aaccggctgc
     3901 tttggtgcgg cacttgccgc ccgcgtcggt accagggttt atcgcgattt cagcgaagcc
     3961 caacgtgact tacagcaccc ggtacgcacc ctgctgccgg atatggccgc ccatcagctt
     4021 taccaacaaa aataccaacg ctatcaacat ctcattgccg cacttcaggg ttatcacgcc
     4081 cgcatcaagg agcacacatt atgagtcgac cacttctgca actggccctc gaccactcat
     4141 cacttgaagc cgcgcagcgc gacgtgacgc tgttaaaaga cagcgtcgat attgtcgaag
     4201 caggcaccat tctctgttta aacgaagggc ttggcgcagt gaaagccttg cgcgaacagt
     4261 gcccggacaa aatcatcgtt gccgactgga aggtcgccga cgcgggtgaa acgctcgcgc
     4321 aacaggcatt tggcgcaggc gctaactgga tgaccatcat ctgcgccgcg ccgctcgcca
     4381 cggtggaaaa aggccacgcg atggcacaga actgcggcgg tgaaattcag atcgagctat
     4441 tcggcaactg gacgctggac gatgcccgtg actggcatcg tattggtgta cgacaggcga
     4501 tttatcatcg tggtcgtgac gcgcaggcca gcgggcaaca gtggggcgag gccgatctgg
     4561 cacgcatgaa ggcgctttcc gatatcggcc ttgagctttc cattaccggc gggattaccc
     4621 ctgctgacct gccgctgttt aaagagatcc gcgtgaaagc gtttattgcc ggacggtcgc
     4681 tggcaggcgc agcaaatccg gcgcaagtgg ccgctgattt tcatgcgcaa atcgactcca
     4741 tctggggagg caagcgtgcg taatcatccg ttaggtattt atgaaaaagc gctggcgaaa
     4801 gatctctcct ggccggagcg gctggtgctg gcaaaaagct gtggttttga ttttgtcgaa
     4861 atgtcggtgg atgaaaccga cgaacggttg tcacgtcttg actggagcgc cgcacaacgt
     4921 gcttcgctgg tcactgcgat gctcgaaacg ggtgttgcta ttccgtcaat gtgcctgtcc
     4981 gcacatcgtc gttttccttt tggtagccgg gacgacgccg tgcgccagcg ggcgcgggag
     5041 attatgagta aagcgattcg cctggcacgc gatctcggca ttcgtactat tcaactggca
     5101 ggttacgacg tctattacga agatcatgac gaaggcaccc agcaacggtt tgctgaaggg
     5161 ctggcgtggg cggttgaaca ggcagcggca tcacaagtca tgctggcggt ggagattatg
     5221 gacaccgcgt ttatgaactc tatcagcaaa tggaaaaagt gggacgaaat gcttgcgtca
     5281 ccgtggttca ccgtttaccc ggacgtcggc aatctcagcg cctggggcaa tgatgttccc
     5341 gccgaactga aactgggcat cgaccgtatc gccgccatcc acctgaaaga cactcaaccc
     5401 gttaccgaac atagccccgg acagttccgc gatgtgccgt ttggcgaagg ctgtgtcgat
     5461 tttgttggca tcttcaaaac gctgcatgag ctgaactatc gcggttcttt cctgattgag
     5521 atgtggaccg aaaaagccaa agagccggtg ctggagatta ttcaggcgcg gcgctggatt
     5581 gaagcgcgta tgcaggaggc cggatttata tgttagagca actgaaagct gacgtgctgg
     5641 cggcaaacct ggcgcttccc gttcatcatc tggtgacgtt cacctggggc aatgtcagcg
     5701 cgatagacga aacgcggcaa tggatggtga ttaagccttc cggcgttgag tatgaggtaa
     5761 tgaccgccga cgatatggtg gtggttgaga tagccagcgg caaggtggtg gaaggcagca
     5821 aaaagccctc ttccgacacg ccaacgcatc tggcgcttta ccgtcgcttt gcggagattg
     5881 gcggcatagt gcatacccac tcgcgccacg ccaccatctg gtcgcaggcc ggacttgatc
     5941 tccccgcctg gggcactact cacgccgatt atttttacgg agccatcccc tgcacgcgcc
     6001 tgatgaccgc agaagagatc aacggtgagt atgaatacca gaccggcgaa gtgatcatta
     6061 aaaccttcga agagcgtggg ttgaacccgg cacaaatccc ggcggtactg gtgcattctc
     6121 acggcccgtt cgcctgggga aaaaatgccg ccgatgcggt gcataacgcc gtggtactgg
     6181 aagagtgcgc ctatatgggt ctgttctcgc gccagcttgc gccacagctc cctgcgatgc
     6241 aaaacgaact gctggataag cactacctgc gtaagcatgg ggccaatgcc tattacgggc
     6301 agtaatccct cacgccgggg cttcatcgcc ccggcactac gaattgatat gttccttgct
     6361 gtaacgccgc ttccacgctg ctggcgttaa gccggtgtgt ttgcgaaaaa tctgccggaa
     6421 atagccgacg tcattaaacc cgcacaacgc cgccacttcc gtgagcgatc gcgcttcgct
     6481 aattaacagc ttttcggctt cgcgcacccg ctggcggtgg attgcttctg ttaacgtcag
     6541 atgaaacacc cgccgataga cgcgcccaag ataatcggca ttgcagtaca gctcgcgggc
     6601 taaaagcgcc gtggaaagcg ggcggtgata ttgcgtgcga atcagtttct gcgctttcca
     6661 ggcaagggtg gtgcctgcac catcggcctg ttcatcaaga cgcgcgtcac gggtgagctg
     6721 ctgcaaaatc agcagcacaa taaactccag cgccggagtg cgttgatgct tttcctgttc
     6781 gcttaagaac tggcgaaaca aggccaccag cacctgcgga tcggcaacct tagtttgttg
     6841 cggcacgcta agatgagtga gccagcgcgg atcgttgtcc ggcgctttaa cctcaaaatg
     6901 cagccagtaa aatttgagat cggcgggaaa atcctccacc ccaacatgct gtcggttcgg
     6961 ccagagtaat aaactctctc ccgcatcgac cagaaatatc cgttcatctt cctgaatcgt
     7021 taatcgacct ttttcgacga agataatttc ccatgactcc agccgccttg caggatgcct
     7081 ccctacgcca cgggaaataa ataatccacc attttgcact ttaatcggta acgtcatgga
     7141 taattcgagc at
//
LOCUS       DACADA010000208         7090 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.210, whole genome shotgun sequence.
ACCESSION   DACADA010000208 DACADA010000000
VERSION     DACADA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.210"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            73..1851
                     /locus_tag="ISB17_004329"
     CDS             73..1851
                     /locus_tag="ISB17_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HAP1982193.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1890..2369)
                     /gene="xdhC"
                     /locus_tag="ISB17_004330"
     CDS             complement(1890..2369)
                     /gene="xdhC"
                     /locus_tag="ISB17_004330"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAP1982194.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2366..3244)
                     /gene="xdhB"
                     /locus_tag="ISB17_004331"
     CDS             complement(2366..3244)
                     /gene="xdhB"
                     /locus_tag="ISB17_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAP1982195.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3255..5552)
                     /gene="xdhA"
                     /locus_tag="ISB17_004332"
     CDS             complement(3255..5552)
                     /gene="xdhA"
                     /locus_tag="ISB17_004332"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAP1982196.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5966..6721
                     /locus_tag="ISB17_004333"
     CDS             5966..6721
                     /locus_tag="ISB17_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP1982197.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6800..6873
                     /locus_tag="ISB17_004334"
     tRNA            6800..6873
                     /locus_tag="ISB17_004334"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6832..6834,aa:Gly,seq:ccc)
BASE COUNT         1776 a         1700 c         1789 g         1825 t
ORIGIN      
        1 aatatcatca tgatctgtga accatcaacg tcttcaatga tcgactttta agtaagtctg
       61 gaaggtaaag ctatggagct tgcgactacg cagtcagtat tgatgcaaat tcaaccaaca
      121 attcagcgtt ttgccagaat gcttgccagc gttttgcagc ttgaggttga gatcgttgat
      181 gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga agtttcttgg ccgccagttg
      241 agcggcaact cacgtctgct ccgccacgtc ctggaaacga aaactgaaaa agttgtgaca
      301 cagtctcgct tcgatcccct ttgtgaaggt tgcgatagta aagaaaattg ccgcgaaaaa
      361 gcatttctgg gtacgccagt cattttacag gatcgttgtg ttggggtgat aagtttgatt
      421 gccgttaccc acgagcaaca agaacatatc agtgataatt tacgcgaatt ttccgattac
      481 gttcgccata tatccaccat ttttgtttct aaacttctgg aggatcaggg accaggagat
      541 aacatcagta aaatattcgc gaccatgatc gataatatgg atcagggcgt gttagtggtt
      601 gatgctgata atcgggttca gtttgttaat cagacagctt taaaaacact cggtgttgta
      661 caaaataata ttattggaaa acctgttcgt ttcagaccat taacatttga gagtaatttt
      721 acccatggtc atatgcaaca tattgtttcg tgggatgata aaagtgaatt aatcattggt
      781 cagttgcata acatccaggg ccgacaacta tttttaatgg cgtttcacca atcgcatacc
      841 agtttttctg tagcaaatgc acctgatgaa ccgcatattg aacaattggt tggcgagtgc
      901 cgtgttatgc ggcaattaaa acgactcatt agccgtattg cacccagccc atccagcgtt
      961 atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg cccgtgcaat ccataagttg
     1021 agcggaagac ggaataaacc ctttattgct atcaactgtg ccgcgattcc ggaacagctt
     1081 ctggagagcg aactgttcgg ttatgttaaa ggcgcattta ctggcgcttc tgccaacggt
     1141 aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt ttctcgatga aattggtgat
     1201 atgccattaa tgttgcaggc taaattactg cgcgctattg aggcgcgtga aattctgccg
     1261 attggtgcca gtagcccaat acaagtcgac atccgcatca tttctgcaac taatcagaat
     1321 ttggcccagt tcattgccga aggtaaattc cgcgaagatc tcttctaccg acttaatgtt
     1381 atcccgataa ctcttccacc gctgcgtgaa cgtcaggaag atattgaatt actagtgcat
     1441 tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat tgctcctgat
     1501 gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt aagcaatttg
     1561 atggaatatc tggttaacgt tgttccttca ggtgaagtta tcgacagcac gctattgccg
     1621 ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggc cagtgaggcg
     1681 cacctgtcac tcgatgatgc gggcggcacg gcgctggaag agatggaaaa gcaaatgatc
     1741 cgcgaggcgc tttcacgtca taacagcaag aaggaagttg ctgatgaact gggcatcggc
     1801 attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata acctctgaga
     1861 atccggcctg aattcaggcc ggatatcttt tactttgttt tctcgcaatc cagaactgta
     1921 tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc acgacgaatt
     1981 tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc
     2041 aggcccggcg tacaaaaccc gcattgcact gcaccagatt tcgcataagc cagttggaca
     2101 tgagaaagtt taccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc
     2161 caggccgcaa ggaataagca actgtctatc gcagtgccgt cgaccagcac cgtacaggca
     2221 ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgttc gcggagtaat
     2281 tccgagagcg gcgtgcctgg tgcggcgtga agctgaaaag gcatcccgtt aatggtgcat
     2341 tcgatggtaa ttgtttcgct gtgattcatt gcaatttccc ccccgccgcg gcgacggctt
     2401 cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc
     2461 gccatgacga acgcggggcg acatcttgca ggactgattc gctgatggct tccagcgttt
     2521 gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg
     2581 gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat
     2641 gtgcggcgca gccaattgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg
     2701 cgctgcccgc gtgttctttc ggctgtggcg gaaaatgaaa ggcgacaagg atttcgtcat
     2761 gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg aaacgaacac
     2821 cgcgcgggga gtggatctcc agtttcgcgt cataaattag cgttggcgtg gcagaatctg
     2881 cgctggtggc accgttgcaa atatttccgc cgtaggtggc gacattacgg atctgcggcc
     2941 cggcgattga tgcagccgca gcacataacg ccgggagatg acgttgagtt acaggatctt
     3001 caatgagctg ggtaaatgtc gttgcagagc cgatacgtag cgcgccatct tccgccagcg
     3061 taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt
     3121 gatggtgaag ctgtatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt
     3181 cagtcagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa
     3241 acatgatgtt atcctcaaat caatcctgcc agatggaatt cttcatataa tcgttttggc
     3301 gttagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagca
     3361 gcggctacag gaattatggg tggctcaccc agtgacttat gtccgtatgc ggattgcggc
     3421 tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg
     3481 taatccagca gattggggtt acggaccacg ccgcttttcg catcgatgat catctcttca
     3541 aatagcgccc agccaatgcc catccccatt ccgccgtgta cctgaccttc tgccagcagc
     3601 ggattaagaa tatggcccga atcatgaacg ttgaggatgc ggttgatggt tactttacac
     3661 agtgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg gttagtggtg
     3721 gttttgatgg agctttcggc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc
     3781 atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg
     3841 ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg
     3901 attttctctt ttaataacag tgccgcactg cgcagcgcag gcgcggcaac atagctctga
     3961 cgtgaggcaa atgcgccggg gtcgaatggt gtaacgtcgg tgtcttgtgt cgaaattacg
     4021 cggacatcgc tgaccggaac tcccacggtt tctgccacca tttgcgagaa cacggtgtcg
     4081 gcaccctggc cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc
     4141 aacaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt gtagctaaaa
     4201 caggcgacgc caacgccacg acgtaaattg ccttgctggt tctggcactc tgcacggcgt
     4261 ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa
     4321 atacgttttc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg taaacgaatt
     4381 tcaacaggat caatacctaa cgctgtcgcg gcatcatcaa gcatagactc aacggcaaat
     4441 acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag
     4501 gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt attcccccca
     4561 gcagaagcaa tggagtgccc gtgagatgca taagcgccgg tgttagacag aacatccaga
     4621 ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat agtaaaagcg
     4681 tggcgggtac gggttgcgag gaaacactct tcacggctaa gagaaacttt caccggaatg
     4741 ccgccaagtt tgcttgtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta
     4801 ccaaaaccgc caccgataaa cggtttgatg actcgtacgc atgaccaggg aatatccagc
     4861 gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt
     4921 cgcgagtcat cctccatcca tgccagcgat gtcacgcttt ccatatgaca atgttgaata
     4981 acgggcgtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca
     5041 ttacccgtcg acatcgtgct ttgtttcagt aaattgccac cgttatggat tggtgcagcg
     5101 tcttctgcca gcgccgcttc tgacgaggtg ataacgggta attcttgcca ctcaatgctg
     5161 accaattgca ccgctttttc tgccgtgagt tcatcgcggg ctacgacgat ggcaacggcg
     5221 tcaccatgat gacgaacatg acgcgttagc agggcacgat cggcagtatc gcgcttgttt
     5281 tcgtcaagtg tccaggcatg ccctgccgtg gcgaatggga tttctggcac atcttcccag
     5341 gtaaaaatcg ccaggacgcc cggcaaactc ctggcttgtt catcattaat acttacggca
     5401 taaccatgtg cgatagggct acgtacatat ttcgcgtaac acatgcccgc cataacataa
     5461 tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catcgacgcg catgcatgat
     5521 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcggtttg aacatttggt
     5581 tcttatggca aagatttagt cttagcaggc aagaattacg ccagaaacga ctatatgcgt
     5641 gaggggagga aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
     5701 ctgaataggg acagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaatta
     5761 ggattattac gcatcaaggt gggaaatagg gtaaaagtca ggaaattcga taccactaca
     5821 gcaaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta
     5881 catcgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat gatatggcgt
     5941 cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg
     6001 tatcgtgatg ttgttatcgg ttggactgct tttggcgggc tgttcgggta gcaaatcatc
     6061 cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg
     6121 tatttcgcgc accacgggaa ccagcgtaaa agaactggcg cgactgaacg gcatttcccc
     6181 cccttacacc attgaagtag gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag
     6241 tacacgtaaa tcaaccgcca aatccacgac caaaaccgca tcggttacac cgtcatcagc
     6301 ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg
     6361 gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc
     6421 tccacgaggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca
     6481 gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta
     6541 cgctcataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat
     6601 cgccactatg gggagcactg atgcggcatc tgttcgcctg catttccaga ttcgttaccg
     6661 tgcaacggca attgatccgc tacgttactt gccgcctcag ggcagcaagc caaaatgctg
     6721 atggcgaatt aatcagcagt cagcagcgtg gtacttgcta aggagagcgt aaggtttata
     6781 atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc
     6841 tatacgaggg ttcgattccc ttcgcccgct ccaatttatc ttcacatcat ctacctgcca
     6901 tttttgttgt atctgatttt ctatattatc ttaattcaag cgcttcagac ttttcgcaat
     6961 cgccgtaata cttccccatt tttttgtaaa taaaaagcgt ttacgcaatg caatcgtcat
     7021 gtatcttaaa tagtcttatt taagatgttc agaactcttt gtgtgtttaa ctggctgata
     7081 attatataat
//
LOCUS       DACADA010000209         6927 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.211, whole genome shotgun sequence.
ACCESSION   DACADA010000209 DACADA010000000
VERSION     DACADA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.211"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1106
                     /locus_tag="ISB17_004335"
     CDS             <1..1106
                     /locus_tag="ISB17_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1982198.1"
                     /translation="VKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCMLIFLALI
                     NKVSENTKQRNLFSKKVTLCISLFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVV
                     WKASYVTIMIFMWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSI
                     WYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQSAS
                     AKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFG
                     VLLTEIDTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIY
                     QLADNALYEAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(1211..1298)
                     /locus_tag="ISB17_004336"
     tRNA            complement(1211..1298)
                     /locus_tag="ISB17_004336"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ser,seq:gga)
     gene            1532..2470
                     /gene="ghrA"
                     /locus_tag="ISB17_004337"
     CDS             1532..2470
                     /gene="ghrA"
                     /locus_tag="ISB17_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAP1982199.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2525..3262
                     /locus_tag="ISB17_004338"
     CDS             2525..3262
                     /locus_tag="ISB17_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAP1982200.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3286..3840
                     /locus_tag="ISB17_004339"
     CDS             3286..3840
                     /locus_tag="ISB17_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1982201.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3942..4433
                     /locus_tag="ISB17_004340"
     CDS             3942..4433
                     /locus_tag="ISB17_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP1982202.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(4498..5331)
                     /gene="csgG"
                     /locus_tag="ISB17_004341"
     CDS             complement(4498..5331)
                     /gene="csgG"
                     /locus_tag="ISB17_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HAP1982203.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5358..5774)
                     /gene="csgF"
                     /locus_tag="ISB17_004342"
     CDS             complement(5358..5774)
                     /gene="csgF"
                     /locus_tag="ISB17_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAP1982204.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5799..6188)
                     /gene="csgE"
                     /locus_tag="ISB17_004343"
     CDS             complement(5799..6188)
                     /gene="csgE"
                     /locus_tag="ISB17_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAP1982205.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6193..6843)
                     /gene="csgD"
                     /locus_tag="ISB17_004344"
     CDS             complement(6193..6843)
                     /gene="csgD"
                     /locus_tag="ISB17_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAP1982206.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1792 a         1537 c         1680 g         1918 t
ORIGIN      
        1 ttgttaaaac cgtcattatc attcagcaaa ttattgaggg gcgtttgaca agcagtgttg
       61 ttcaaaatga cattgcaatt tattatttgt ttcgtcagat gagtttgtgc atgttaatat
      121 ttctggcatt gataaataaa gttagtgaaa acacaaaaca gcgcaattta ttttccaaaa
      181 aagtgacttt gtgcataagt ttgttttttg ttcttggggg gccgatagtt gctcacatat
      241 tttctagcca ttatgagagc tacgatttac acattgccga gttaaccaat gaaaatggtc
      301 aggtggtttg gaaagcctca tatgttacca taatgatttt catgtggtta accttgcttt
      361 cggttaatct atactttaat ggtttacgct gtgatatctg gaacggggtg tcggtgattg
      421 ctttttgtgc cgtgctgtac aatatctctc tgttatttat gagcaggtat agcgtgtcca
      481 tttggtatat tagtcgcact attgaagtag tcagtaagtt aaccgttttg gtaattttta
      541 tgtgccatat attcagtgcg ctacgagtaa caaaggacat tgcacatcgc gatcccttaa
      601 ccaatatatt caacagaaat tattttttta atgaactgac ggttcaatca gcatcggcca
      661 aaaaaacacc ttattgcgtc atgattatgg atattgacca cttcaaaaaa gtcaacgaca
      721 cctggggaca tccggttggc gatcaagtga taaagacagt ggtgaatatc attgggaaaa
      781 gcatacgacc agatgacctt ttagcgcgcg ttggcggcga agagtttggc gtcttgctga
      841 cggagatcga tactgaatgt gcgaaagctt tagcggaaag gattcgggaa aatgttgaac
      901 gtttaactgg cgataatcct gaatacgcca taccacaaaa agtgacgatt agtattggcg
      961 ctgttgtaac gcagaaaaat gagttaaacc ctaaggagat ctatcaactg gctgataacg
     1021 cactttacga ggcgaaagag accgggcgta ataaggtggt agtaaaagag gcagaaattc
     1081 ttatcaaccg caaggatgat gaatgagtct gtcgctagtt gtcaacctcc gcctggggct
     1141 tctcatcccc ccggtgtatg caatatacga aaaaaaagct cgtactttcg tacgagctct
     1201 tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
     1261 ttccaggcgt gctccttcag ccactcggac acctcaccaa attgtcgttc ctgtcttgct
     1321 ggaacgggcg ctaatttagg gaaatcatga cctgaggtca acaaactttt ttaaaaaatc
     1381 gctcgattat tcaaacttca atcaatatgt agttttaata agcgaaattt gctttttttg
     1441 tcaccgacca cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat
     1501 aaatttcgcg caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt
     1561 cgatacccaa tggtggattg aggcactgca caaagctatt cctcaggcaa gagtcagagc
     1621 atggaaaagc ggagacaatg aatcagccga ttatgcttta gtctggcatc ctcctgttga
     1681 aatgctggca gggcgcgatc ttaaagcggt gtttgcactt ggggcaggtg ttgattctat
     1741 tttgagcaag ctacaggctc accctgaaat gctgaagcct tctgttccac tttttcgcct
     1801 ggaagatacc ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg
     1861 gtttcgacgt tttgacgatt accgcctcca gcaaaatagt tcgcattggc aaccgctgcc
     1921 tgaatatcat cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggcagtaa
     1981 agttgcacag agtctgcaaa cctggcgctt cccgctgcgt tgctggagtc gaacccgtaa
     2041 atcgtggcct ggcgtgcaaa gctttgccgg acgggaagaa ctttctgcat ttctgagcca
     2101 atgccgggta ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca
     2161 acaattactc gaaaaattac cggatggcgc gtatctcctc aacctggcgc gtggtgttca
     2221 tgtcgtggaa gatgacctgc tcgcggcgct ggatagcggc aaagttaaag gcgcaatgct
     2281 ggatgttttt aatcgtgaac ccttaccgcc ggaaagtccg ctctggcaac atccacgcgt
     2341 gacgataaca ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg attacatttc
     2401 gcgcaccatt gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg
     2461 cggctactaa taaagcatca ggtttcctgc tatccttggc gggaattgaa tacaggagag
     2521 agttatgtat cccgtcgacc ttcatatgca taccgttgcc agcacgcatg catatagcac
     2581 attgagtgat tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga
     2641 tcatggcccg gatatggaag atgcgccgca tcactggcac ttcattaaca tgcgtatctg
     2701 gccgcgagtg gttgatgggg tagggatcct gcgcggcatc gaagctaaca ttaaaaatgt
     2761 tgatggtgaa attgattgca gcggcaaaat gtttgattcg ctggatctga ttattgccgg
     2821 ttttcatgag ccggtttttg cgccacatga caaagcaacc aatacacaag cgatgatcgc
     2881 cactatcgcc agcggcaatg tgcatataat aagccatccc ggaaatccga aatatgagat
     2941 agatgttaaa gccgttgctg aagcagccgc gaaatatcag gtggcgctgg aaatcaataa
     3001 ttcctcattt ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagctgcagc
     3061 ggtacgtgac gctggaggtt gggtggcatt aggctcggat tctcacactg catttaccat
     3121 gggggaattt gaagagtgtc ttaaaatcct cgacgcggta gattttccgc cagaacgcat
     3181 tttgaatgtt tctccgcgcc gcttactgaa cttccttgaa tctcgcggta tggcaccgat
     3241 tgcggaattt gcagaccttt aattactagt aatggaaata tattaatgaa cgagttttct
     3301 atcctctgtc gtgtgctggg ttcgctctat taccgccaac cgcaagatcc tttactggtg
     3361 ccgttgttta ccctgattcg tgaggggaaa ctggctgcga actggccact ggagcaggat
     3421 gagttactga cgcgtttaca gaaaagctgt gatatgacgc aagtctctgc cgattacaat
     3481 gcgttgttta tcggcgatga atgtgctgtg ccgccatatc gtagcgcatg ggttgagggc
     3541 gcaacggaag cggaagtgcg cgcttttctt tccgaacgag ggatgccact agcggatacg
     3601 ccagccgatc acatcggcac attgctgctt gcggcttcct ggctggaaga tcagtcaacg
     3661 gaagatgaga gcgaagcact ggaaacactg ttcagtgagt atctgttacc ctggtgtggt
     3721 gcgttccttg gcaaagtgga ggcccatgca actacgcctt tctggcgcac catggcaccg
     3781 ctaacccgcg atgctattag tgcaatgtgg gacgagctgg aagaagattc tgaagagtaa
     3841 atgtgatctg catcactcat aactgcaact gataatttgt tgttgcataa aatgtgtgct
     3901 cgatctcatt catggccgcg ttttctgcta tcatgcgcgg catgaacata cttctctcta
     3961 ttgcaatcac aacaggcatt ctctccggta tctggggatg ggtggctgtt tccctgggct
     4021 tactaagctg ggcgggcttc ctgggctgta cggcctactt tgcctgcccg caaggtgggc
     4081 tgaaagggct ggcgatctcc gctgcaaccc tgcttagtgg cgtggtgtgg gcgatggtca
     4141 ttatttacgg tagtgcactg gcaccacatc tggaaattct cggttatgta ataaccggta
     4201 ttgtcgcgtt tctgatgtgt attcaggcca aacagctgct gctttcattt gttccgggga
     4261 cgtttatagg cgcatgcgcg acgtttgccg ggcaggggga ctggaaactg gtgttacctt
     4321 cgctggcgct tggactggta tttggttacg caatgaaaaa cagcggcctg tggctggcgg
     4381 cgcgtagtac gaagaccgct caccgtgagc agcaaatcaa aaataaagcg tgaggggcac
     4441 tcacgctttc gcttaaacag taaaatgccg gattattaat tccggcgttt ttatctatca
     4501 ggattccggt ggaaccgaca tatggcggta tttcaccaga atgtcattct gccgttctgt
     4561 tttattttgc aaatcccaaa ggccgcggtc gataccatca ttaatcagga aaatgacgcc
     4621 tgtttcgata gccgacatca ggcacagcat aacgggttcg ttcgaggtgt aacctacttc
     4681 cccttccagc aagcgctggt agtcaataaa gcggaagacc cccgcctgaa cttcatagga
     4741 aagtatcgtc ttactggtgt tcaccgagga gaggatctca ccggtactca cattgacgac
     4801 acgcaggttc acggcaatct gatcaagctg gtattgcgtg tctgcaccga tcccaaaata
     4861 tcttgcccca acaccgcccg atttaacgtt gctttcataa ccgataatcg aaccttcaac
     4921 catgatattt gccgccgtta aagattgcag cgggattcgg ttattaatgg caactgtgcc
     4981 gttttcttgt gccgcacgaa taatcttgcg ctcgttaagc agattttgta agccctggcg
     5041 ctccagcggt ataaaccagc gagaatcttt cagtgccgtg accagcattg ccgtggcgct
     5101 ttgcggaaca gcagtggaga agttacttgc cgggtagggt ttaaattgtc cggtttcgtc
     5161 ctgaatgttg tataccgaaa caaagatctt acccgtcggc gctggcagat gggtcaaatc
     5221 tttgtagctc tgagcacgag gcattaatgt cggtctggcg gcttctttag gcggggcggt
     5281 taagcatccg ctcagtaaca tgacggcaac caaaagaaat aagcgctgca tgattatttt
     5341 ccttatgaag ctggggctta aaaatcggtt gagttatttt gtaaacccga aacctggatg
     5401 gtcgaggttt gtccggtttt acgatctgtc acgttcaact gcaattgacc atcgcggtta
     5461 gcaatatcaa caatataatc gttggtcacc atgcggcccg gtttaccggt attaatattc
     5521 gacagtagcc cacccaaaat ttgtgactgg atggcctgag taaagttatc taacgctgat
     5581 ggcgtttcaa taccaaagtc atcgttatag ctcggatctt tataagagtt ttgtgcctga
     5641 gcgctattta acaaaaaagc gccattattt gggttaccac caaagtttgg attacggaac
     5701 tggaaagtca tggatccagc ccaacttaat ggcgaaataa gcatgagtag aactactgca
     5761 tgtttgacac gcattgcagc ctccgaacaa tttattattt aaaattcatc atgcgccaaa
     5821 tcgcccgtac ttaataacgc ctggtttatc tggcgacgat ttagtgcttc ttcagtttga
     5881 atcagtgcaa agacgacagt tttctcgaag tctcttttca acggaaataa aaaagtctgg
     5941 aaaataacgt cctgattgac cgttatagtg atccaacttc cccatcgtgc actgggcctt
     6001 tcattaatcg ttaagttacc cgtatagtca ctttcccatt tatcactaaa ggctcggtaa
     6061 aaatcatggc caatagatga aacagtatgg tcagttaaca atcccgggac ttctacctca
     6121 acggcatgaa gattccctgc ggcgaacaga aattctgccg ccacaatcca gcgtaaataa
     6181 cgtttcatgg ctttatcgcc tgaggttatc gtttgcccag gaaaccgctt gtgtccggtt
     6241 ttttacggct atcttcttga aaagattata aagatgcgtt ttaaccgtat tttcgctgat
     6301 gaacaacgaa cgagcgatct cgttattaga cgcgccgata cgcagcttat tcaggatctc
     6361 tttttcccga tgagtaagga gggctgattc cgtgctgtta taacgatagt tacctgaatg
     6421 cgtaatcagg taactggcaa gcttttgcgt aaagtagcat tcgccgcgca ggacgccttg
     6481 caacccattg acaacacgtt cttgatcctc catggcataa aaaacgccgt tgatatgagg
     6541 ccagttttca atgtcgcggt acgggtaatc ttcaggcgta tttagcaaca atattttgat
     6601 attgttgttt ttcctgctca aagtatcctg ccaataatgg ataagctttt tatccgcttc
     6661 catcatatcc agaagaataa tagagcctga agatatatcg tccagagaac gttgaatatt
     6721 atgtaatttt cctgtaatcg ccagcgattg tttaaggtgc tgcaagagag ctgtcgcttg
     6781 caaagaaggt ttagtgatca acaataatgt atgaccatga atactatgga cttcattaaa
     6841 catgatgaaa ccccgctttt ttattgttcg cacacctgac agctgcctct aaaatagaag
     6901 caccagaagt actgacagat gttgcac
//
LOCUS       DACADA010000210         6899 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.212, whole genome shotgun sequence.
ACCESSION   DACADA010000210 DACADA010000000
VERSION     DACADA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.212"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            460..1449
                     /locus_tag="ISB17_004345"
     CDS             460..1449
                     /locus_tag="ISB17_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1982207.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1519..3033
                     /locus_tag="ISB17_004346"
     CDS             1519..3033
                     /locus_tag="ISB17_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAP1982208.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTNMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3045..4034
                     /locus_tag="ISB17_004347"
     CDS             3045..4034
                     /locus_tag="ISB17_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAP1982209.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4201..5001
                     /gene="otsB"
                     /locus_tag="ISB17_004348"
     CDS             4201..5001
                     /gene="otsB"
                     /locus_tag="ISB17_004348"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAP1982210.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4976..6400
                     /gene="otsA"
                     /locus_tag="ISB17_004349"
     CDS             4976..6400
                     /gene="otsA"
                     /locus_tag="ISB17_004349"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAP1982211.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6407..6835)
                     /gene="uspC"
                     /locus_tag="ISB17_004350"
     CDS             complement(6407..6835)
                     /gene="uspC"
                     /locus_tag="ISB17_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAP1982212.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
BASE COUNT         1719 a         1619 c         1813 g         1748 t
ORIGIN      
        1 attatacgtg ttctgttaat taatgagcac agtgataatt aatggcgaaa tgaatgacgc
       61 gcattttcca catctttaag ttgcggttat tacaccattg caaaaactca acgccaggta
      121 atgaggccta ttgactggtt aaaaagaaga catcccgcat gggtaccaaa gacaacaagg
      181 acttccaggc taatcttatg gattattctg ctgtgcactc gataatttgt ctgacaaatt
      241 cgcttttcct tatgtctttt cccgctaaat ttatgcacgt tctcactgta attctgcgat
      301 gtgatattgc tctcctatgg agaattaatt tctcgctaaa actatgtcaa cacagtcact
      361 tatcttttag ttaaaaggta atgctttgtt ttccgattaa tttaacgaat gtcattcgtt
      421 tttgccctac acaaaacgac actaaagctg gagagaacca tgcacaaatt tactaaagcc
      481 ctggcagcca ttggtctggc agccgttatg tcacaatccg ctatggcgga gaacctgaag
      541 ctcggtttcc tggtgaagca accggaagag ccgtggttcc agaccgaatg gaagtttgcc
      601 gataaagccg ggaaggattt agggtttgaa gttattaaga ttgccgtgcc ggatggcgaa
      661 aaaacattga acgctatcga cagcctggcc gccagtggcg cgaaaggttt cgttatttgt
      721 actccggacc cgaaactcgg ctctgccatc gtggcgaaag cgcgtggcta cgatatgaaa
      781 gtcattgccg tggatgacca gtttgttaac gccaaaggta agccaatgga taccgttccg
      841 ttggtgatga tggctgcgac caaaattggc gaacgtcagg gccaggaact gtataaagag
      901 atgcagaaac gtggctggga tgtcaaagaa agcgcggtga tggcgattac ctccaacgaa
      961 ctggatacgg ctcgtcgccg tactacgggg tctatggatg cgctgaaagc tgcaggattc
     1021 ccggaaaaac aaatttatca ggtaccgacc aaatctaacg acatcccggg ggcatttgac
     1081 gctgccaact caatgctggt tcaacatccg gaagttaaac attggctgat tgtcggtatg
     1141 aacgacagca ccgtgctggg cggcgtacgc gcgacggaag gtcagggctt taaagcggcc
     1201 gatatcatcg gtatcggtat taacggtgtg gatgcggtga gtgaactgtc taaggcgcag
     1261 gcaaccggct tctacggttc cctgctgcca agcccggacg tacatggtta taagtccagc
     1321 gaaatgcttt acaactgggt tgcaaaaggt gttgaaccga ccaaattcac cgaagttacc
     1381 gacgtggtgc tgatcacgcg tgacaacttt aaagaagaac tggagaaaaa aggtttaggc
     1441 ggtaagtaat ttgccggaaa aattcccctc tgcatgatgc agagggggtg tgaacgacca
     1501 gtgattcacg gagacgttat gcaacagtct accccgtatc tctcatttcg cggcatcggt
     1561 aaaacgtttc ccggcgttaa ggcgctgacg gatattagtt ttgactgcta tgccggtcag
     1621 gttcatgcgt tgatgggtga aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc
     1681 ggaaactatg cgccaaccac gggttctgta gtgattaatg ggcaggaaat gtccttttcc
     1741 gacacgaccg cagcacttaa cgcgggtgtg gcgattattt atcaggaact gcatctcgtg
     1801 ccggaaatga ccgtcgcgga aaatatctat ctcggccagc tgccgcataa aggcggcatt
     1861 gtgaatcgtt cattgctgaa ttatgaggca ggtttacaac ttaaacatct tggtatggat
     1921 attgacccgg acacgccgct gaaatatctc tccattggtc agtggcagat ggttgaaatc
     1981 gccaaagcgc tggcgcgtaa cgccaaaatt atcgcctttg atgagccaac cagctccctc
     2041 tctgcccgcg aaatcgacaa tcttttccgc gttattcgtg aattgcgaaa agaggggcgg
     2101 gtgatcttat acgtttctca ccgtatggaa gaaatatttg ccctcagcga tgccatcacc
     2161 gtctttaaag atgggcgtta tgtcaaaact tttaccaata tgcagcaggt tgaccacgac
     2221 gcactggtgc aggcgatggt cgggcgagac attggcgata tctacggctg gcaaccgcgt
     2281 agttatggcg aggagcggct gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca
     2341 ataagtctgg cggttcgcag tggagaaatt gtcggtctgt ttggtctggt cggggcaggg
     2401 cgtagcgaat taatgaaagg catgtttggc gggacgcaaa tcaccgccgg tcaggtttat
     2461 atcgaccaac agccgatcga tattcgtaaa ccgagccacg ccattgccgc aggcatgatg
     2521 ctctgcccgg aagatcgcaa agcggaaggc attattcccg tgcactccgt tcgcgacaat
     2581 atcaacatca gtgccagacg taaacatgtg cttggcggtt gtgtaatcaa caacggttgg
     2641 gaagaaaaca atgccgatca ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag
     2701 caactgatca tgaatctctc aggcggaaat cagcaaaaag ccattctggg ccgctggtta
     2761 tcggaagaga tgaaggtcat tttgctggat gaacctacgc gtggcattga tgttggtgct
     2821 aagcatgaaa tttacaacgt gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt
     2881 gcctccagcg acttacctga agtcctcggc gttgccgacc ggattgtggt gatgcgggaa
     2941 ggcgaaatcg ccggtgaatt gttacacgag caggcagatg agcgtcaggc actgagcctt
     3001 gcgatgccta aagtcagcca ggcagttgcc tgagtaagga gagtatgatg tcttctgttt
     3061 ctacatcggg ttctggcgca cctaagtcgt cattcagctt cgggcgtatc tgggatcagt
     3121 acggcatgct ggtggtgttt gcggtgcttt ttatcgcctg tgccattttt gtcccaaatt
     3181 ttgccacctt cattaatatg aaagggttgg gcctggcaat ttccatgtcg gggatggtgg
     3241 cttgtggcat gttgttctgc cttgcttccg gtgactttga cctttctgtc gcctccgtaa
     3301 ttgcctgtgc gggtgtcacc acggcggtgg ttattaatct gactgaaagc ctgtggattg
     3361 gcgtggcagc ggggttgctg ctgggcattc tctgtggtct ggtcaatggc tttgttatcg
     3421 ccaaactcaa aataaatgcc ctgatcacga cactggcaac gatgcagatt gttcgaggtc
     3481 tggcgtacat catttcagac ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc
     3541 ttggttacgc caactggttc ggtctgcctg cgccaatctg gctcaccgtc gcgtgtttga
     3601 tcatctttgg cttgctgctg aataaaacca cctttggtcg taataccctg gcgattggcg
     3661 ggaacgaaga ggctgcgcgt ctggcgggtg taccggttgt tcgcaccaaa attattatct
     3721 ttgttctctc tggcctggta tcagcgatag ccggaattat tctggcttca cgtatgacca
     3781 gtgggcagcc aatgacgtcg attggttatg agctgattgt gatctccgcc tgcgttttag
     3841 gtggcgtttc tctgaaaggc ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa
     3901 ttttaggcac cgtggagaac gccatgaacc tgcttaatat ttctcctttc gcgcagtacg
     3961 tggttcgcgg cttaatcctg ctggcagcgg tgatcttcga ccgttacaag caaaaagcga
     4021 aacgcactgt ctgatgcttt tttctgcaac aatttagcgc ttttttcctc cacagccagc
     4081 cgccacaacg gttggctgtt cttcgttaca aatggcgacc cccgtcacac tgtctatact
     4141 tacatgtctg taaagcgcgt tctgcgcaac acaataagaa aagagaagga ggagaaccgg
     4201 gtgacagaac cgttaaccga aacccctgaa ctatccgcga aatatgcctg gttttttgat
     4261 ctcgatggaa cgctggcgga aatcaaaccg catcccgatc aggtcgtcgt gcctgacact
     4321 attctgcaag gacttcagct actggcaacc gcaagtgatg gtgcattggc attgatttca
     4381 gggcgctcaa tggtggagct tgacgcactg gcaaaacctt atcgcttccc attagcgggc
     4441 gtgcatgggg cggagcgccg tgacatcaat ggtaaaacgc atatcgttca tctgccggat
     4501 gcgattgcgc gtgatattag cgtgcaactg catacggtca tcgcccagta tcccggcgcg
     4561 gagctggagg cgaaagggat ggcttttgcg ctgcattatc gccaggctcc gcagcatgaa
     4621 gacgcattaa tgacattagc gcaacgtatt actcagatct ggccacaaat ggcgttacag
     4681 cagggaaagt gtgttgtcga gatcaaaccg aaaggtacca gtaaaggtga ggcgattact
     4741 gcctttatgc aggaagctcc ctttatcggg cgaacgcccg tatttctggg cgatgatcta
     4801 accgatgaat ctggcttcgc agtcgttaac cggctgggcg gaatgtcagt aaaaattggc
     4861 acaggtgcaa ctcaggcatc atggcgactg gcgggcgtgc cggatgtctg gagctggctt
     4921 gaaatgataa ccaccgcatt acaacaaaaa agagaaaata acaggagtga tgactatgag
     4981 tcgtttagtc gtagtatcta accggattgc accaccagac gagcacgccg ccagtgccgg
     5041 tggccttgcc gttggcatac tgggggcact gaaagccgca ggtggactgt ggtttggctg
     5101 gagcggtgaa acagggaatg aggatcagcc gctaaaaaag gtgaaaaagg gtaacattac
     5161 gtgggcctct tttaacctca gcgaacagga ccttgacgaa tactacaaca aattctccaa
     5221 tgccgttctc tggcccgctt ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc
     5281 ctgggatggc tatctacgcg taaatgcgtt gctggcagat aaattactgc cgctgttgca
     5341 agacgatgac attatctgga tccacgatta tcacctgttg ccctttgcgc atgaattacg
     5401 caaacgggga gtgaataatc gcattggttt ctttctgcat attcctttcc cgacgccgga
     5461 aatcttcaac gcgctgccga catatgacac cttgcttgaa cagctttgtg attatgattt
     5521 gctgggtttc cagacggaga acgatcgtct ggcgttcctg gattgccttt ctaacctgac
     5581 ccgcgtcacg acacgtagcg caaaaagcca tacggcctgc ggcaaagcgt ttcgaacgga
     5641 agtttacccg atcggcattg agccgaaaga aatagcgaaa caggctgccg gtccactgcc
     5701 gccaaaactg gcgcaactca aagcagaact gaaaaacgta caaaatatct tttctgtcga
     5761 acgactggat tattccaaag gtttgccgga gcgttttctc gcctatgaag cgttactgga
     5821 aaaatatccg caacatcacg gtaaaattcg ttatacccag atcgcgccaa cgtctcgtgg
     5881 tgatgtgcaa gcctatcagg atattcgtca tcagctcgaa aatgaagccg gacgaattaa
     5941 tggtaaatat gggcaattag gctggacgcc gctttattat ttgaatcagc attttgaccg
     6001 taaattactg atgaaaatat tccgctactc tgacgtgggc ttagtgacgc cactgcgtga
     6061 cgggatgaac ctggtggcaa aagagtatgt tgccgctcag gacccagcca acccgggcgt
     6121 tcttgttctt tcgcaatttg cgggagcggc aaacgagtta acgtcggcgt taattgttaa
     6181 tccctacgat cgtgacgaag ttgcagctgc gctggatcgt gcattgacta tgtcgctggc
     6241 ggaacgtatt tcccgtcatg cagaaatgct ggacgttatc gtgaaaaacg atattaacca
     6301 ctggcaggag tgcttcatta gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca
     6361 gcagcgcgat aaagttgcta cctttcctaa gcttgcgtag gtgcggttaa cctcccgtaa
     6421 gtggaactaa cagcacatcg acctcacttg aggtaatcac tcttttcgcc gagcaggatg
     6481 ctcgcgaaaa gaaactgtga ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgc
     6541 gacaaacttc cagaatatgt tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg
     6601 gataccccgc atcctgaatt aacttatcaa gaaagctttg cgtttcttcc tgcatcacgt
     6661 tgcgtaaatc ttcaagcatc ggcgcagcta attgattgta catttccggg tcggaagcga
     6721 gagtaattaa actgatgtgt ccctttactg gcctggcgat agataccgct tttgccagca
     6781 gttgctgact ttccggtgta accgcaacgg cgacaagaat attgctatag ctcataacct
     6841 gttccttatt ctgtgaactt caggtgacat taaagcttta ctcctaatgg agaaacgac
//
LOCUS       DACADA010000211         6892 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.213, whole genome shotgun sequence.
ACCESSION   DACADA010000211 DACADA010000000
VERSION     DACADA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.213"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            110..1507
                     /gene="ulaA"
                     /locus_tag="ISB17_004351"
     CDS             110..1507
                     /gene="ulaA"
                     /locus_tag="ISB17_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP1982213.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFASRAL
                     RAEEDAEKQLAEQSA"
     gene            1523..1828
                     /gene="ulaB"
                     /locus_tag="ISB17_004352"
     CDS             1523..1828
                     /gene="ulaB"
                     /locus_tag="ISB17_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAP1982214.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1838..2302
                     /gene="ulaC"
                     /locus_tag="ISB17_004353"
     CDS             1838..2302
                     /gene="ulaC"
                     /locus_tag="ISB17_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAP1982215.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2316..2966
                     /gene="ulaD"
                     /locus_tag="ISB17_004354"
     CDS             2316..2966
                     /gene="ulaD"
                     /locus_tag="ISB17_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP1982216.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2976..3830
                     /locus_tag="ISB17_004355"
     CDS             2976..3830
                     /locus_tag="ISB17_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP1982217.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            3830..4516
                     /locus_tag="ISB17_004356"
     CDS             3830..4516
                     /locus_tag="ISB17_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1982218.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFVWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4645..4920)
                     /locus_tag="ISB17_004357"
     CDS             complement(4645..4920)
                     /locus_tag="ISB17_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1982219.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5248..5643
                     /gene="rpsF"
                     /locus_tag="ISB17_004358"
     CDS             5248..5643
                     /gene="rpsF"
                     /locus_tag="ISB17_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP1982220.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5650..5964
                     /gene="priB"
                     /locus_tag="ISB17_004359"
     CDS             5650..5964
                     /gene="priB"
                     /locus_tag="ISB17_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAP1982221.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5969..6196
                     /gene="rpsR"
                     /locus_tag="ISB17_004360"
     CDS             5969..6196
                     /gene="rpsR"
                     /locus_tag="ISB17_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP1982222.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6238..6687
                     /gene="rplI"
                     /locus_tag="ISB17_004361"
     CDS             6238..6687
                     /gene="rplI"
                     /locus_tag="ISB17_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP1982223.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1650 a         1711 c         1886 g         1645 t
ORIGIN      
        1 cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt gagagcacaa
       61 cattccgggt gtgcgaaata cccggttacc tcttcttcag gagatcgtta tggagatcct
      121 ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg
      181 tattgtgacc tgtctgggtt acatcctact gcgcaaaagt gtcagcgtta ttattaaagg
      241 tacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca tcctgaccag
      301 caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc
      361 cgatacttac gcgtcgatga tggcaaccat tgaacgcatg ggcgatgcct atagctgggt
      421 gggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat
      481 taccggtatt cgaaccatca tgctgactgg tcacatcatg ttccagcagg cggggctgat
      541 tgccgttacg ctgtttatct tcggctactc catgtggacg accattatct gcaccgcgat
      601 tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga cacaggaagt
      661 caccgacggc tgtggtttct ccattggtca ccagcagcag ttcgcatcat ggattgctta
      721 taaagtcgca ccgttccttg gcaaaaaaga ggaaagcgtt gaagacctca aactgcctgg
      781 ctggctgaat attttccacg acaacatcgt ctccacggcg attgtcatga ctatcttctt
      841 tggtgccatt ctgctctcct ttggtatcga caccgtgcag gcgatggcag gcaaagtgaa
      901 ctggacggtg tacatcctgc aaaccggttt ctcctttgcg gtggcgatct tcatcatcac
      961 tcagggcgtg cgcatgtttg tggcggaact ctctgaagca tttaacggta tctcccagcg
     1021 tctgatccca ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgcgccgaa
     1081 cgccgtggtc tggggtttta tgtggggcac catcgggcaa ctgattgcgg ttggtatcct
     1141 ggtcgcctgt ggctcttcaa tcctgattat tcccggcttt atcccgatgt tcttctccaa
     1201 cgccactatc ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaaaatttg
     1261 tctggtgatg gggatgatcg aaatctttgg ttgcgtctgg gcagtgaaac tcaccggtat
     1321 gagtgcctgg atgggtatgg cggactggtc gattctggca ccgccgatga tgcagggctt
     1381 cttctccatc ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt
     1441 cttcgctagc cgcgcgctgc gcgcagaaga agatgcagaa aaacaactgg cagaacaatc
     1501 tgcttaataa ggagttttga ttatgaccgt tcgtattctg gctgtgtgtg gtaacggaca
     1561 aggcagttcc atgatcatga aaatgaaagt ggaccagttt ttaacccagt cgaacattga
     1621 ccacacggta aacagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat
     1681 catcatcgct tctacccata ttgcgggcga aatcaccgtg accggcaaca aatatgtggt
     1741 tggcgtgcgc aacatgctct ctcctgctga ttttggcccg aaactgctgg aagtgatcaa
     1801 agagcatttc ccgcaggacg tgaagtaagg acgcgccatg aaattacgtg attcgctggc
     1861 tgaaaataaa tctatccgcc tgcaagcgga agcagagaca tggcaggacg cagtgaaaat
     1921 cggcgttgat ctgctggtag cggcagatgt ggtagagccg cgttactacc aggcgattct
     1981 ggatgcggtt gaacagcacg gcccgtactt tgtgctcgcc cccggcctgg caatgccgca
     2041 cggacgcccg gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc
     2101 gctggagttc aaccacgaag ataacgatcc ggtggatatc ctcatcacca tggcggcggt
     2161 tgatgccaac actcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga
     2221 agagaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga
     2281 ccgcaccaac gcggcagctt aagaaggaat tgaacatgtc attaccgatg ttgcaagtcg
     2341 cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag
     2401 tcgacattat cgaagtgggc accattcttt gcgtaggcga aggcgtgcgt gcggttcgtg
     2461 acctgaaagc gctgtacccg cacaaaatcg tactggctga cgccaaaatt gccgatgcag
     2521 gcaaaatcct ttctcgcatg tgcttcgaag ccaacgctga ctgggtgacc gtaatttgtt
     2581 gtgcggatat caacaccgcc aaaggcgcgc tggacgtggc aaaagagttt aatggcgacg
     2641 tgcagatcga actgaccggc tactggacct gggaacaggc gcagcagtgg cgcgacgcag
     2701 gcattgggca ggtggtttat caccgcagcc gtgacgcgca ggccgcaggc gtggcgtggg
     2761 gcgaagcgga cattaccgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca
     2821 ccggtggtct ggcgctggaa gatctgccgt tgtttaaagg cattccgatt catgtgttta
     2881 tcgctggtcg cagcatccgt gatgcagcct ctccggttga agccgcgcgt cagttcaaac
     2941 gttccatcgc cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg
     3001 gcatctacga aaaagcgctc cccgctgggg agtgctggct ggaacgactg caactggcaa
     3061 aaacgttagg cttcgatttt gtcgagatgt cagtagatga aacggacgat cgcctttcgc
     3121 gcctcgactg gagccgcgag cagcgtctgg cactggtgaa cgcgattgtt gaaaccggcg
     3181 tgcgcgtacc gtccatgtgc ctttctgctc atcgtcgttt cccgctggga agcgaagatg
     3241 acgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc tatccagttc gcccaggacg
     3301 tcggcattcg cgtgatccaa ctggcgggct atgacgttta ctatcaggaa gccaataacg
     3361 aaacgcgtcg tcgttttcgt gacggcctga aagagagcgt tgagatggca agccgcgcgc
     3421 aggtcacgct ggcgatggag atcatggatt acccattgat gaactccatc agcaaggcgc
     3481 tgggctacgc acactatctc aacaatccgt ggttccagct ctacccggat atcggcaacc
     3541 tgtcggcgtg ggacaacgac gtgcagatgg agttgcaggc cggaatcggg catatcgtcg
     3601 cggtacatgt gaaagacacc aaaccaggcg tcttcaaaaa cgtgcctttt ggcgaaggtg
     3661 ttgtggattt tgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc
     3721 tgattgagat gtggagcgaa acggcggaag atccggcggc agaagtggcg aaagcgcgcg
     3781 attgggtgaa agcgcgcatg accaaagcag gcatggtgga ggcggtataa tgcaaaagct
     3841 gaaacagcag gtgtttgagg ccaacatgga tctgccgcgt tacgggctgg tgacctttac
     3901 ctggggcaac gtcagcgcca ttgaccgcga acgcgggctg gtggtgatca agcccagcgg
     3961 cgtcgcctac gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggtaaggt
     4021 ggtggaaggg gagtatcgtc cgtcttccga cactgcgacg catctcgaac tctaccgtcg
     4081 ttatccgtcg cttggtggta ttgtccatac ccactccact catgccaccg catgggcgca
     4141 ggcggggctg gcgatcccgg cgttaggcac cacgcacgcc gattacttct ttggcgacat
     4201 cccgtgcacg cgcggattaa gcgaagaaga ggtgcagggt gagtacgaac tgaataccgg
     4261 caaagtgatt atcgaaacgc tgggtgacgc cgagccgctg catacgccgg gaattgtggt
     4321 gtatcagcac ggaccgttcg tctgggggaa agatgcccac gatgcggtgc ataacgcggt
     4381 ggtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaacc cacaactgaa
     4441 tcacatcgac agctttctga tgaataaaca cttcatgcgt aagcacggtc ccaacgccta
     4501 ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cttcgggaga taactggtct
     4561 aattccgcag ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca
     4621 atgtttcgtt ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc
     4681 gttactcccc ggttcccgca tcagaatcac atggtagtac gttgcgcctt gcgcatctgt
     4741 ctcttcattt aatgcctgac gtgcttcact ttcagtggcg aagttatggt tgatataaat
     4801 cacacctaag ctttgcacat catccatatt tctggcctgg tggttattaa tttcaatggc
     4861 tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaacac gactgaacat
     4921 agatacctcc tcgacggctg actttgtgtg ctctcctctt gtggtgatct tctgatttaa
     4981 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac
     5041 accatgacga agtgtgatga actccaaatc agcgatttag aggttatttg cgaaaggggc
     5101 gatttatttc tgctcagccc ttgagtttag cggggtatgt cagtactata acgcgtcatt
     5161 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
     5221 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
     5281 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
     5341 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
     5401 aacaaactgc acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
     5461 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
     5521 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
     5581 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
     5641 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
     5701 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
     5761 gaggaagccg gctttcaccg gcaggcgtgg tgccaaatgc ccgttattgt tagcggacac
     5821 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
     5881 atttcatgcc ataaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
     5941 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
     6001 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
     6061 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
     6121 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
     6181 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
     6241 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
     6301 aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag
     6361 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
     6421 ctggcagcag ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
     6481 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
     6541 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
     6601 ctgcgtacca ctggtgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
     6661 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc
     6721 agtggtcggc gttttgcttt taagtgataa aaaccgcaga aacagacgtt ccctccataa
     6781 atctcttgct attcctttta taaaaagttc gcagtaccca tacctggccc ccagcctcgc
     6841 cgctggtagt tcgtgtcatg ttttttacgc gtaaaacacg acaaaagcat ca
//
LOCUS       DACADA010000212         6872 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.214, whole genome shotgun sequence.
ACCESSION   DACADA010000212 DACADA010000000
VERSION     DACADA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.214"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            2..1711
                     /gene="adeD"
                     /locus_tag="ISB17_004362"
     CDS             2..1711
                     /gene="adeD"
                     /locus_tag="ISB17_004362"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP1982224.1"
                     /translation="MLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQPVSASDFALQFTPGKRYRVI
                     EVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLE
                     VTE"
     gene            complement(1758..3149)
                     /gene="uhpT"
                     /locus_tag="ISB17_004363"
     CDS             complement(1758..3149)
                     /gene="uhpT"
                     /locus_tag="ISB17_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAP1982225.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3287..4606)
                     /gene="uhpC"
                     /locus_tag="ISB17_004364"
     CDS             complement(3287..4606)
                     /gene="uhpC"
                     /locus_tag="ISB17_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAP1982226.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4616..6118)
                     /gene="uhpB"
                     /locus_tag="ISB17_004365"
     CDS             complement(4616..6118)
                     /gene="uhpB"
                     /locus_tag="ISB17_004365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAP1982227.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6118..6708)
                     /gene="uhpA"
                     /locus_tag="ISB17_004366"
     CDS             complement(6118..6708)
                     /gene="uhpA"
                     /locus_tag="ISB17_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAP1982228.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         1730 a         1978 c         1798 g         1366 t
ORIGIN      
        1 attgttagcc gtttcccgtg gagacgctgt tgccgattat attattgata atgtctctat
       61 tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag gacgttacat
      121 tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg atgcccgcgg
      181 cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca gcatgatgac
      241 gccggtcacc tttgaaactg ctaccctgcc gcgcggcctg acgaccgtta tttgcgaccc
      301 tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc gctgtgccga
      361 acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc tggaaggctg
      421 cgatgttaat ggtgccagtt ttacccttga acagatgctc gcctggcggg accatccgca
      481 ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc agaatgcgct
      541 gctcgataaa ctggacgcat ttcgccacct gacactggac ggtcactgcc cgggtttggg
      601 tggtaaagaa cttaacgcct atattgctgc gggtattgaa aactgccacg aaagttatca
      661 gttggaagaa ggacgccgga aattgcaact cggcatgtca ttgatgatcc gtgaagggtc
      721 cgcagcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca gcccgcaatg
      781 catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac acatcgatgc
      841 cttaattcgc cgcctgatcg aacaacacaa tgtaccgctg catgtggcat atcgcgtagc
      901 cagctggtcg acggcgcgcc attttggtct gaatcacctc ggcttactgg cacccggcaa
      961 gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc aggtactggt
     1021 gaaaggcgag ccgatcgatg cacaaacctt acaggcggaa gagtcggcga gactggcaca
     1081 atccgccccg ccatatggca ataccattga tcgccagcca gtttccgcca gtgactttgc
     1141 cctgcaattt acccccggaa aacgctatcg cgttattgag gtaatccata acgaactgat
     1201 cacgcactcc cgctccagcg tctacagcga aaacggtttt gatcgcgatg atgtgtgctt
     1261 tattgccgtt cttgagcgtt acggacaacg actggctccg gcctgtggtt tgctcggcgg
     1321 ctttggcctg aatgaaggtg cgctggcggc gacggtcagc catgacagcc ataatattgt
     1381 ggtgatcggt cgtagcgcag aagagatggc gctggcggtc aatcaggtga ttcaggatgg
     1441 cggcgggctg tgcgtggtcc gtaacggcca ggtacaaagt catctaccgt tgcccattgc
     1501 cgggctaatg agcaccgaca cggcgcagtc actggcggag caaattgacg ccttgaaagc
     1561 cgccgcccgt gaatgcggtc cgttaccgga tgagccgttt attcagatgg cgtttctttc
     1621 cctgccagtg atccccgcgc tgaaactaac cagtcagggg ctgtttgatg gtgagaagtt
     1681 tgccttcact acgctggaag tcacggaata ataaaaaaaa gcccggcgtc atgccgggca
     1741 aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc gcggcggatt
     1801 ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga tacaaccaat cgcggcgata
     1861 tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccaa ataccggcgt cccatcggca
     1921 atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcgaa ggtgccttta
     1981 ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagccgc cacgccaatc
     2041 aacagttgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag gtagatatat
     2101 tggttgctgg cgtgttgata cacaccgagc gtggcgataa tcagcgccag cgcgatacag
     2161 gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag cagcgtaccg
     2221 accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc
     2281 agttcctgga acgcatacac agttgaccac tggtcgatac caatacgcac cacatagagg
     2341 aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata ctcaacaaag
     2401 atctgccact tggtcatatc ggtagattct gtctctttat cctcttcact gatctcctcg
     2461 ccaaacagct cttcagcttt gccgaggcca taagattccg gggagtcgct gccgtaacgc
     2521 agaccgataa aaccaacaat cagcgcgata atcgacggga aaataaacat gccgatgaca
     2581 tgaccatcga acaggtaatt tgcgccgaac agcgccacac ctgctgcgcc tgcaccacca
     2641 aggttgtgag aaatgttcca gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat
     2701 ttggtgatgg tggagtaact gcatgaaccg ccggtactct ggaaaaagcc gctcaaggcg
     2761 tagaaggcaa tcatcaggaa caggctaacc gagccgctgc ccatactggc actgaagccc
     2821 agcatacaaa tggcagacag gatcagcatg aacggcagga attgtttggt gtttttgccg
     2881 tcggcgtagt aagaaaccag cgttttaccc acgccataag tgatggagaa gcccaggccg
     2941 atcatcccca gctgcgtcat gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg
     3001 ttaaagttct tgcgaatcag gtacatcgta aggtagccga taaagaccac caggtaggat
     3061 tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc cagggtcggc
     3121 ttgcgaacct ggtttaagaa agccagcatg ggttactcct gaaatgaata cctgcgtgag
     3181 gcatgcattg taaaaatcag cagcaaatca ggcctgagat agcgtccagg caaaacctgg
     3241 aaaatttctt agttttgccg gatatgaagt gaaaaggtga gatgcatcac gcttctcgcg
     3301 gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg gcgatggcga
     3361 taaccacaaa aaatccgctc cagtgccagg tatcgagtac tttcgccagc ggccaaccag
     3421 caagcgacgc accaagataa gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct
     3481 ctttgtggga acactctgcc gccgccatgc cgattaacat ctgcgggcca aagacaaaaa
     3541 aaccaatggt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc atcagccaca
     3601 gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc ccgcggttgc
     3661 cgttaaacag tttgtccgaa ccccaacccg ctaccagcgc accgataaat ccgcccagtt
     3721 caaacattgt cactgccgta ttcgccgtga ccagatcgac gcccagcgtc tcggacatat
     3781 acaaattgcc ccagtcgttg atcgccgccc gaaccacata gaccagcaca tagcaaaacg
     3841 aaagcagcca gatatacgga ttcagcaaca catatttggt gaggatctct ttacgcgtca
     3901 gccctgcccc ttcttgttgt tgagcaatct ccagtgcgtc gtgccgccat tcaccaactg
     3961 ccggtaaacc taacgcctgc gggcgatcgc gcagccgcca gcagagaaaa atccccacga
     4021 cgatcgccat acaacccgca atcatcatcc cggcacgcca gccgtaatgc agcgcgctcg
     4081 ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc cataatgccc
     4141 accaaccgcc gcgctcggta cgagaatacc aggcggttaa cagacgcgca cacaccggtg
     4201 aaccccagcc ctggaaaaag gcgttcaaca cccagagcac ggcaaacgcc catagcgacg
     4261 tcgagaagcc aaacagaatg ttgataatgc ccgtggcaat aagccctatc cccataaaat
     4321 aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacaca ccgtaggtaa
     4381 tgtaaaacag ggtcgctaac aggccaatat cgctacggct gagcacgccg ttagcaagga
     4441 tttctggtac ggcggagtta aaacttttac gcgtgaagta aaacagcgcg taacccagcc
     4501 agatggtcag cagaatatgc cgacgccagt agcgatagcg ggcatcaatt tcatgtttat
     4561 cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc gaaccttaga
     4621 cataacgttg aggtagagaa acgctgacac gcgtgccgtg caggcaggag atggtcaatg
     4681 tgccacccag cgccgttacg cgctcgcgca ttccggtaag gccaaaaccg tgttgcccgg
     4741 aatctggcgg taaaccgctg ccatcgtctt caatcaccag cattaaccgc tcatcatgct
     4801 gccagccttg cagcgtgacc gcgctggcat cggcatgttt cacaatgttg ttcaaccctt
     4861 cctggcagac gcgaaatagc gtcacgcgct ggttttcgct taacgctgat tcatcgattc
     4921 gccattcgag atgactgaca atgccgcgcc cttccagctc catttcccgc atcagtgagc
     4981 ggatggcctg ctccagggtg agatcatcca gctggcgcgg acgtaaccgc cctaacaagc
     5041 ggcgcaccgc gtcgtaaacg cccaacgaca actgttcgat gagctgcccg ctctgtttca
     5101 cgctggcgtt atccgccgcg agccgctgga caatgccagc ctgagtacga atagcagtga
     5161 tggtctgacc gatatcatcg tgcagctctc gcgccacatc gcggcgcacg ctctcttcgg
     5221 tttccagcaa ccgttcagcg agatgctgat tgcgcgccag ttccttttgc agcgactggt
     5281 taagttcacg caaccgctgg atgccagcgc ccagtaacaa ccctgtcaga ctttgcacca
     5341 gcagcgagag caataaatcc accggatgat cgcgccaggt ttgactggcg atcagcgcga
     5401 tggcgttcat caatgtcgca atcagcgctc cttgccaacc atagtgccag gccagcgcga
     5461 taatcggcag cgccagacag aatggcgtaa agcgcgacag ttcgtctggc aatcccaact
     5521 ggagccagag actgataaca aacagcagca agtaccagac cagatgtcgc ccgcgccagt
     5581 tgattggctg agaaaccagt gacgggccga gcggcagcca ggtgttattg gcgagatagt
     5641 gccagaacac cagacatatc ggggccagcg tcaggccgcc agttaaagtc agcagcagcg
     5701 cattccacga ctctttgccg tgccaaagcc agggcaacga ctgcaacaac gccgccgccg
     5761 ttaacgccgc accctgtaac agcaaggtgc gccagtcacg ttgatggcga tagcgcgaga
     5821 tcagcgccac gggcaataac gtcagtaaac taccgatcat caataaggag aaatgggtta
     5881 aaccgaccgc ctgcattagc cagtaaatca gcagccactc cgcacccagt agcaccggcc
     5941 agtatccgcg cgggcattgc agcattagcc ctagccgcag gccaaacgga aataacagca
     6001 ccgccatatc agggcgctca accaggtgca ggctgatact ccacaggcaa aaccatgcgg
     6061 cagagaagat aaaaaagcag gcaataacgg taattaaacg ggagaacaac gtcttcatca
     6121 ccagccatca aacatgcggc gcgccagttc aacgtcgtta ctgacgccca gtttttccat
     6181 cagattggcg cgatggacgt gtactgtttt cggcgacaag cccagttcgg cggcaatctc
     6241 tttcaccgcc attccttgcg ccagtttttc tgccacctgg cgttcgcgtt tggttagcgg
     6301 gtcctggcga ccagatgcca gtttaatggc aatatccggc gtcagataac agccgccagt
     6361 ggcaaccgta tgcaccgcag caatgagttc atccgggcta cagcgtttgg agagaaagcc
     6421 gcgcgccccc gcgttaagcg cctgctcaac cagcgccgga ctgtcgtgaa cggagagcat
     6481 aattgtcgcc atgcctttcg gcagctggct tagaagctcc agaccggaga tatcaggcat
     6541 ggagatatcg caaatacaca cctgcacacc gcgccccggc agccccgcca gcgcctcgcg
     6601 ccccgaacca aactcggcaa ctacctgcaa atcaggttcc agccccagca gctgcgcaaa
     6661 gccggagcgg acgataaggt gatcgtctat aagggcaacg gtgatcatgg tcttgtcctg
     6721 gcgggtaata aaacgcgctt accttaacga taagcgcgat gttgttcaag ccttgagcgg
     6781 ttactgccaa aagctgcgct cttgttgctt gtggaaagaa gtgatgcctt caaggacttg
     6841 tatatcgact aaccattaaa aatgctggcg gc
//
LOCUS       DACADA010000213         6665 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.215, whole genome shotgun sequence.
ACCESSION   DACADA010000213 DACADA010000000
VERSION     DACADA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.215"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1094
                     /gene="emrK"
                     /locus_tag="ISB17_004367"
     CDS             <1..1094
                     /gene="emrK"
                     /locus_tag="ISB17_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAP1982229.1"
                     /translation="FIALSGAYAYWSMELKDMISTDDAYVTGNADPISAQVSGSVTVV
                     NHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASAR
                     IQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAYKANKALVMNT
                     PLNRQPQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPA
                     RQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
                     ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSM
                     PAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1094..2632
                     /gene="emrY"
                     /locus_tag="ISB17_004368"
     CDS             1094..2632
                     /gene="emrY"
                     /locus_tag="ISB17_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAP1982230.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2740..4068)
                     /gene="dsdA"
                     /locus_tag="ISB17_004369"
     CDS             complement(2740..4068)
                     /gene="dsdA"
                     /locus_tag="ISB17_004369"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAP1982231.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4086..5423)
                     /gene="dsdX"
                     /locus_tag="ISB17_004370"
     CDS             complement(4086..5423)
                     /gene="dsdX"
                     /locus_tag="ISB17_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HAP1982232.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            5641..6576
                     /gene="dsdC"
                     /locus_tag="ISB17_004371"
     CDS             5641..6576
                     /gene="dsdC"
                     /locus_tag="ISB17_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HAP1982233.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1837 a         1530 c         1541 g         1757 t
ORIGIN      
        1 tatttattgc gctttcaggt gcctatgcct attggtcaat ggaattaaaa gacatgatta
       61 gtacagatga cgcctatgtc acgggaaatg cagatccaat ttctgcacaa gtctcaggca
      121 gtgtcactgt cgttaatcat aaagatacga actacgttcg acaaggtgac attttagttt
      181 cactggataa aactgatgcc actatcgcac tcaataaagc taaaaataat ctggcaaata
      241 ttgttcggca aacgaataaa ctatacttac aggataaaca atacagtgcc gaagtcgctt
      301 cagcacgtat tcagtatcaa caatctttag aagattataa ccgtcgtgtg cctttagcga
      361 agcaaggggt tatttcaaaa gaagcgctgg agcataccaa agatacgtta ataagtagca
      421 aagcggcatt gaatgctgct atccaggctt ataaagcgaa taaagcttta gtaatgaaca
      481 caccattaaa ccgtcagcca caagtcattg aagctgcaga tgcaaccaaa gaagcctggt
      541 tggcgcttaa acgtacggat attaagagtc cggttaccgg ttatattgcc cagagaagtg
      601 ttcaggtcgg cgaaacagtg agtcccggac aatcgttaat ggctgtcgta ccggcgcgtc
      661 aaatgtgggt taatgccaac tttaaagaaa cacaactcac ggatgtacgg attggtcaat
      721 cggtcaatat tatcagcgat ctttatggtg aaaatgttgt gtttcatggt cgggtgacag
      781 ggatcaatat gggaaccggc aatgcgttct ccttattacc tgcacaaaat gcgacaggga
      841 actggatcaa aatcgttcag cgtgtaccgg ttgaagtttc tctggatcca aaagaactca
      901 tggaacaccc tttgcgcatt ggtttatcga tgactgcaac tattgatacg aagaatgaag
      961 acattgccga gatgcccgat ctggcttcaa ccgtgacgtc catgcctgct tataccagta
     1021 aggctttagt tatcgatacc agtccgatag aaaaagaaat tagcaacatt atttcgcata
     1081 atggacaact ttaatggcaa tcactaaatc aactcctgca ccattaaccg gtgggacgtt
     1141 atggtgcgtc actattgcat tgtcattagc gacgtttatg caaatgttgg attccactat
     1201 ttctaacgtc gcaataccga caatatctgg ctttctggga gcatcaacag acgaaggtac
     1261 ctgggttatc acctcgtttg gcgtagcaaa tgccattgcg atccctgtta ccggcaggct
     1321 ggcacaaaga ataggcgaat taaggttatt tttactttca gtaacctttt tttcgctgtc
     1381 ttcattaatg tgtagtttat cgaccaatct tgatgtgctg atatttttta gagtcgttca
     1441 ggggttaatg gcggggccgt taattccact gtcacagagt ttattattaa ggaattatcc
     1501 accagaaaaa agaacatttg ctctggcatt atggtcaatg accgtgatta tcgctccgat
     1561 atgtgggccg atattgggcg gctatatttg cgataacttt agctggggtt ggatattttt
     1621 aatcaatgtc cctatgggga ttatcgtcct gacattatgc ttaaccttac ttaaaggacg
     1681 agaaactgag acttcaccgg tcaaaatgaa tctaccagga ctgaccctgt tagtgctcgg
     1741 tgttggtggc ttgcaaatta tgcttgataa agggcgcgat ctggattggt tcaactcgag
     1801 tacaataata ctactaacag tagtatcagt tatttctctg atctctttag tcatttggga
     1861 gtcgacctca gagaatccga ttcttgacct cagtttgttt aagtcccgta atttcaccat
     1921 tggtattgtg agtatcacat gcgcgtattt attttactct ggagcgatag ttcttatgcc
     1981 gcagttactc caggaaacga tggggtataa tgcgatatgg gccggacttg cctatgcgcc
     2041 catcggtatc atgccgctat taatatcacc tttgatagga cgttatggca acaaaattga
     2101 tatgcggttg ttggtgacat ttagtttttt gatgtatgcg gtttgctatt actggcgttc
     2161 tgtgacattt atgccaacga ttgattttac aggcatcatt ttgccacagt tttttcaggg
     2221 attcgccgtt gcctgtttct ttttaccctt aacaacgatt tcgttttcag gcttgccaga
     2281 taataaattt gccaatgcct cgagtatgag taattttttt cgcaccttgt caggatcagt
     2341 tggtacgtcg ttgacaatga cactgtgggg acgccgcgaa tcgttacacc atagtcagtt
     2401 gacagcaacc atcgatcaat ttaaccccgt gtttaattca tcgtcacaaa ttatggataa
     2461 atactatggt tcgctttcag gagttcttaa tgaaattaat aatgaaataa ctcagcagtc
     2521 actttctatt tctgcaaatg agattttccg tatggcggct attgctttta tcttacttac
     2581 ggttttggtt tggtttgcga aaccgccgtt tacagcgaaa ggcgttgggt gatggaaaaa
     2641 gaggaggcta tagcaggtaa aacggcagaa tcttaagtct gcgtcatcgg cagcatcgct
     2701 cacccaggga aaggatggtg atgctgcgtt gaaacgttat taacggcctt ttgccagata
     2761 ttgattcatc tcttcttccg gcaccattcc acctcccgtc gcccacacca gatgagtggc
     2821 attacgcagt tgttctgcgc tgaaaccgtg catctgttgg taacttactg atgcgcacac
     2881 gcgctgaggt ccggccatac ccgccagtgc cgaaggttca agacgaatac cttcttcctg
     2941 cgccagccag ccaagcatgt catacatggt ttgatcgcta agggtataga agccatccag
     3001 cagacgctcc atcgcccgcc cgacaaagcc cgatgcgcga ccaaccgcaa ggccatcggc
     3061 agcggtaagg ttgtcgatgc caatatcctg aacagaaatc tgatcgtgta atccggtatg
     3121 gacgcctaat aacatacacg gggagtgtgt tggttcggca aaaaagcagt gaacatgatc
     3181 gccaaacgcc agtttaagtc cgaatgcgac gccaccagga ccaccgccaa caccacacgg
     3241 cagatagaca aatagagggt tatcagcatc gacgatacgg ccttgctggg caaattgcgc
     3301 tttaagacgc tggccagcga cggaataccc aaggaacaac gtgcgggaat tctcgtcatc
     3361 aataaagaaa cagttcgggt cagactgcga tgctttacgt ccttcctcga cagcaacacc
     3421 ataatcttgc tcatattcca cgaccgtaac gccatggctg cgcagtttcg cttttttcca
     3481 tgcccgggca tcagcagaca tatgcactgt caccttaaag ccaatgcggg cgctcataat
     3541 accgattgat aaccccagat ttccggttga gcccacagca atgctgtatt ggctaaagaa
     3601 ctgtttaaac tccggagaaa gcagtttgct gtagtcatct tcaagcgtca gcaaccctgc
     3661 ttccagagcc agtttttctg catgtgccag gacttcataa atcccgccgc gtgcttttat
     3721 ggagccggaa ataggcaaat ggctatcttt tttcagtaac agttgcccgc tgatcggttg
     3781 ctgatattcc ttttccagcc gtttttgcat agccggaatg gcaaccagtt ctgattcaat
     3841 aatcccccca gtggcagcag tttcaggaaa tgcttttgcc agataggggg caaaacgaga
     3901 taagcgcgca tgggcgtcct gaacatcctg ttcggtcagg ccaacataag gtaaaccttc
     3961 agccaatgag gtcgtgccag gattgaacca agtggtttct ttaagagcaa ccagatcctt
     4021 taccaacgga tactgggcga tgagcgaatt cattttagcg ttttccatag tacgtctctt
     4081 tgcgcttaga tgataaatga cagcaggaat gtgcccgcca gagcgatgac cgaagcgata
     4141 aatgtcgctg tcgtatagta tttaaatgtt tcattgagcg tagcgccgca atattgcttc
     4201 accagccaga aaagcgagtc cgtaacgatc gtgcagccaa tcgcaccgga accgatagca
     4261 atcgcaataa tttccgggct gatgtcggga tacagcggca gcatgggggc aacaattgcc
     4321 gttgccccca tcattgccac cgtagcggag cccactgcag catgcagaat aagtgccacc
     4381 aaccaggcca gaagaatcgg gtgcatatgc atattggaga gaataactgc cagcgtatca
     4441 gcgagactgc tgctttttaa aatggcgttg aatgcgcctc cggccccgat aatcagcaaa
     4501 atattagcaa tagaaccgaa accattttcc gtatgtgtga gcatcgtccc catgctcata
     4561 tgctgacgta tacccaacac ataataggcg acaaacacgg cgataaacat ggcagtgata
     4621 gggttgccaa taaactcaag caaggtatac aaaccactct cacgcgccat attcaattcg
     4681 gcaatcgttt taaccaacat cagcgcaatg ggtagcagta tggtgaataa cgttgcgcct
     4741 aatgaaggta gtgttttttc atcgcgaact ttgagatcgg caaactctgt gggtacaggt
     4801 ttaaagggca gtcgttgccc cagaaattta aggaaaagtg ggccaccgat cagtgatgcc
     4861 atcagcccaa ccagcaaacc gtagacgatc accgaaccga tatctgcgcc cagcttattg
     4921 gcaacatata aagcagccgg atgtggggga accacgcagt gcacagccat caatgcggta
     4981 cacagcggaa tggccagctt taacaatgag gtattggttt ttttggcaat tgaaaaagcc
     5041 agaggaatca atagcacgac gcccacttca acaaacagcg tgatgccgca aatcaggcca
     5101 accagcacca taatgacatc agccgaaagc caccggcagc gttgaagtgt cagaccaatt
     5161 ctttctgcgg ccccggatac ttccatcatt tttcccagta tcgtgccaag gccgataacc
     5221 gcagcgagga accccagcgt tccgccaatt ccactttcaa tagcatttac catatccagt
     5281 ggccccatac ccatcatcgt tcccacgaag aagctggcca gcaacagcgc cagaaacggg
     5341 tggaatttga acttcacgat ggtcagtaca attaacacga tgctgataag cagcgtgctc
     5401 acaacccaga tttgagagtg catatctcac cttcccctgt gattaacctg atgatattgg
     5461 acaaaaaaac aacgcaggct gacaaacgat aaaatttgcc tttcacatga gctaaattgc
     5521 atcaagggag tgatttacat ctaaaaatga aggtgaattg agatatggtt cactttagct
     5581 caccttacag ctgaaatttt gccgctattt ttttacactt aatagaaaaa tgaggtgatt
     5641 atggaacccc ttcgtgaaat aagaaatcga ctgcttaacg gctggcaact atcaaaactg
     5701 catacttttg aagtggctgc caggcatcag tctttcgccc tggcggcaga ggaattgtcg
     5761 ctgagcccca gtgcggtaag tcaccgtatc aatcagctgg aagaagaatt aggtattcag
     5821 ttgtttgttc gttcccatcg caaagtggaa ttaacgcacg aggggaaacg cgtttattgg
     5881 gcgctaaaat cgtcgctgga taccctgaat caggaaattc tggatatcaa aaatcaggag
     5941 ttatcgggaa cgttaactct gtattcccgg ccctctatcg cccaatgctg gttggtgccc
     6001 gcattaggtg actttacacg ccgatatccg tctatttcgc tcaccgtgct cactggtaat
     6061 gacaatgtca atttgcaacg tgccggaatc gatttggcga tctactttga tgatgcgccg
     6121 tcagcgcaac tgacccataa ctttctgatg gatgaagaaa tcttgccagt ttgtagcccg
     6181 gagtacgctc atagacatga tttaaccaac acggtaatta acctgcgtca ctgtacgttg
     6241 ctccatgaca gacaggcatg gagcaacgac tccggtacgg atgaatggca tagttgggcg
     6301 caacattatg cggttaattt gccgacatct tctggaattg gctttgatcg ttctgattta
     6361 gccgttatcg ccgcgatgaa tcatgttggg gtggcgatgg gaaggaaacg cctggtacaa
     6421 aaaaggcttg ccagtggtga gctcgtcgcg ccgtttggcg atatgacggt gaaatgccat
     6481 cagcattatt acatcaccac attaccgggc aggcagtggc caaaaattga ggcatttatt
     6541 acttggttaa gagaacaggt aaaaaccact tcataatgaa taagaccacg agttcctggt
     6601 gtgccttatt cggcactgct agtgcgaatt taataattta cggtttttac gtggttttag
     6661 ggata
//
LOCUS       DACADA010000214         6562 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.216, whole genome shotgun sequence.
ACCESSION   DACADA010000214 DACADA010000000
VERSION     DACADA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.216"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(326..1975)
                     /gene="pgi"
                     /locus_tag="ISB17_004372"
     CDS             complement(326..1975)
                     /gene="pgi"
                     /locus_tag="ISB17_004372"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP1982234.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2500..3849
                     /gene="lysC"
                     /locus_tag="ISB17_004373"
     CDS             2500..3849
                     /gene="lysC"
                     /locus_tag="ISB17_004373"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAP1982235.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4102..4374
                     /locus_tag="ISB17_004374"
     CDS             4102..4374
                     /locus_tag="ISB17_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAP1982236.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4375..5247)
                     /gene="rluF"
                     /locus_tag="ISB17_004375"
     CDS             complement(4375..5247)
                     /gene="rluF"
                     /locus_tag="ISB17_004375"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAP1982237.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5595..6542
                     /locus_tag="ISB17_004376"
     CDS             5595..6542
                     /locus_tag="ISB17_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAP1982238.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
BASE COUNT         1624 a         1623 c         1668 g         1647 t
ORIGIN      
        1 aatcatgaag ttaaaataaa ataaaaagta attactctgc gtattttccg aaataaacca
       61 cgattaaaaa tttattttag gattaacctc acggtatgat tttcgttaaa tgacagacaa
      121 gaggatttat ctgataaaac aatgccgact tatagtcggc attgttttta aggctattta
      181 atcaatcgta ctatgtatta attgccgaat acgatgtaca tatcggcatc gacctgtagg
      241 cctgataaga cgcgaccgcg tcgcatcagg catcggttgc cggatgcggc gtgaacgcct
      301 tatccggcct acatatcgac aatgattaac cgcgccacgc tttatagcgg ttaatcagac
      361 cattggtcga gctatcgtgg ctgctgattt ctttgtcatc tttcagctct ggcagaatac
      421 ggttcgccag ttgtttaccc agctccacgc cccattggtc gaaggtgaag atgttcagga
      481 tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgccccc aggctgaacg
      541 gagtgatttc acgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg
      601 gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca
      661 cttcgcggga tttaccaaac gccagcgctt cggtctgagc aaagaagtta gacagcagtt
      721 tctggtgatg atcagagagc gggttatggg tgatagccgg agcgatgaaa tcgcacggta
      781 ccattttggt tccctggtgg atcagctgat agaacgcgtg ctgaccgttc gtgccaggtt
      841 caccccagat aatcgggcca gtctggtaat ccacaacctt accgttacgg tcaacatact
      901 taccgttgga ttccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt
      961 catacggcag aatcgcttca gtttccgcgc caaagaaatt gttgtaccag atgccaatca
     1021 gcgccagcag tacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg
     1081 cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca
     1141 ggccaatcgc tgaccacaaa gaataacgac cgccgaccca gtcccagaat tcgaacatgt
     1201 tggcagtatc aataccaaac tcgccaacgg ctttggcgtt ggtggaaagc gccgcaaagt
     1261 gtttcgcaac gtgcttctca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg
     1321 cgttggtcat ggtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt
     1381 ccgggtttac ttttttcagc acttccgcga tgtgagtccc atcgacgtta gaaacaaagt
     1441 gcatgttcag gtggtttttg tatggacgca gtgcttcagt caccatgtac gggccgaggt
     1501 cagaaccgcc gatcccgatg ttcactacgt cagtgattgc cttgccggta tagcctttcc
     1561 actcaccgga aataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt
     1621 ccggcataac atctttgcca tcaaccagaa tcggggtatt gctacggtta cgcagcgcta
     1681 cgtgcagtac ggcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa
     1741 tcgcgcccgc cagatcgcac tctttcgcca gatcttgtaa tttcgccagc gtctcttcag
     1801 tgatgcggtt tttggagtaa tccaccagca tctgatcgtc gaaggttgcg gagaacttag
     1861 aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga
     1921 agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca
     1981 atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa
     2041 tgtgatggtt ttagtgccgt tagcgtaatg ttgagtgtaa acccttagcg cagcgaagca
     2101 tttattagct gaactactga ccgccaggtg tggatgaaaa atccgcatga ccccaccgtt
     2161 gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga
     2221 agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg
     2281 ggtgcatcgc cgaggtgatt gaacggctgg ctacgttcat catcggctac aggggctgaa
     2341 tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt
     2401 ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg
     2461 tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc
     2521 aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg
     2581 ctttctgatg ccaacgtacg tttagttgtc ctctcggctt ctgctggtat cactaatcta
     2641 ctggtcgctt tagctgaagg actggaacct ggcgagcgat tcgaaaaact cgacgcaatc
     2701 cgcaatatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag
     2761 attgaacgcc tgctggagaa cattaccgtt ctggcagaag cggcggcgct ggcaacgtct
     2821 ccggccctga cagatgaact ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc
     2881 gagatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt
     2941 accaacgacc gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg
     3001 ctgcagctgc tcccacgcct caatgaaggc ttagtgatca cccagggatt tatcggcagc
     3061 gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac ggcagccttg
     3121 ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac
     3181 accaccgatc cacgcgtggt ttctgcagca aaacgcattg atgaaatcgc gtttgccgaa
     3241 gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca
     3301 gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg
     3361 ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat
     3421 cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggttt cctcgcggaa
     3481 gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac atcagaagtg
     3541 agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg
     3601 caatccctgc tgatggagct ttccgcactg tgccgggtgg aagtggaaga gggtctggcg
     3661 ctggtcgcgt tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa agaggtattc
     3721 ggcgtactgg aaccgttcaa cattcgcatg atttgttacg gcgcatccag ccataacctg
     3781 tgcttcctgg tgcccggcga agatgccgag caggtggtgc agaagctaca ttttaatttg
     3841 tttgagtaaa cacagtatgg cccggaagac attatttcgg gccgtattgt ttttcttgtc
     3901 attatgccca taaataaacg agcctgtact ctgttaacca gcgtctttat cggagaataa
     3961 ttgcctttaa ttttttatcc gcatctctaa ctaattatcg aaagagataa atagttaaga
     4021 gaaggcaaaa tgaatattat cagttctgct cgcaaacgaa ttcccgtaca ctgaactctc
     4081 tggctcactg agggcaccat catggcactc ccccgtatta cccaaaaaga gatgactgaa
     4141 cgcgagcagc gtgaacttaa aacactgctg gatcgggcgc ggattgcgca tggtcgcgta
     4201 ctgaccaact ccgaaaccaa cagcattaag aaagagtaca tcgataaatt aatggtcgag
     4261 cgtgaggctg aagcgaaaaa agcccgccaa ttgaagaaaa agcaggctta taaaccggat
     4321 cctgaggcgt catttagctg gtcagcaaat acgtcgacgc gcggcaggcg ctgattaacg
     4381 ccccttcttt ttgcgccccg gcgaggtaaa acgcttaccg ttggacgccg ggcgaccgcc
     4441 tttttccgcc gttttttcca tcttaacgac tggacgtttg atgcctaccg ttttcggttt
     4501 ggccttcgct ttaggttttg cctcggaaga ggaattttca atgagcttaa agagatcgat
     4561 taactcatcg tcggttaaat cgcgccactc acccagcgga atgccgctta agctgacgtt
     4621 cataatgcgc gtacgttcca gctttttcac ttcgtaacca aagtgctcgc acatgcgacg
     4681 aatctgacgg ttcagcccct gcaccagggt aatgcggaag acaaacggtg cttctttttt
     4741 gactttgcac tttttagtta ccgtcccgag gatcggcact cccgccccca taccacgaat
     4801 aaattcatcg gtaatcggtt tatcaaccgt caccagatac tctttctcat gatcgttacc
     4861 cgcacggagg atcttattca ctagatcgcc gtgattggtg aggaaaatca gcccctggga
     4921 atctttatcc aggcggccaa tcgggaacac gcgtttgctg tggttaacga aatcgacaat
     4981 gttatcgcgc tcgccatctt cggtggtgct gacaatgcct acgggcttgt tcagggcgat
     5041 aagtaccaaa tcttcggctt cccgaggctc aatcaactga ccgtttactt tcacaacgtc
     5101 gccggatttc acctgatcgc caatggtggc tcgcttgcca ttaaggaaca cattgccttg
     5161 ctcgatatag cgatccgctt cgcggcgtga gcaaattccg ctttcgctga tgtatttatt
     5221 taaacggact gatgagtcgg gcagcataaa ttctcctgta aaacgcggaa tataccgtac
     5281 ctcttagcgg ggaaaaacag ggttatgcgg caacgtcgaa attaaatgtg atctgccacg
     5341 ccttttatca caaaagtgtc tccggaagta cgatgcaaat ttgcctggca aaataaggat
     5401 aatcaacaaa ttacgaatcg ctgcacacct gtgctgatga ctactacgca gagaaacaaa
     5461 tgtgcaagag aaagcgctat ttccccccat gcaaagagtg aaaaatcgct ttgaaaggcc
     5521 atcatgatgg ggttatatca gatcaagcgg tgagaaagcg tatttgcaca aatgtgcaga
     5581 accaggaggc ggcaatggaa aacagtgacg atatccgttt gattgtgaag attgcccaac
     5641 tctattacga acaggatatg acgcaggcgc aaattgcgcg cgaactgggt atttaccgca
     5701 ccaccatcag ccgcttgctt aaacgaggcc gcgatcaggg aattgtcacc atcgccatca
     5761 actatgacta caacgaaaat ctctggctgg agcagcaagt gaagcaaaag tttggcctga
     5821 aagacgttgt ggtggtgtca ggaaatgatg aggatgaaga tactcaactg gcgatgatgg
     5881 ggttacacgg cgcgcaactg ctggatcgct tgctggaacc tggtgatatt gttggttttt
     5941 cctggggtcg cgcggtgagc gcactggttg aaaacttgcc gcaggcgggg caatcgcggc
     6001 agttaatttg cgtgccgatt attggcggcc cgtccggtaa actcgaaagc cgctatcacg
     6061 taaacacatt aacctacagc gcggcagcga agctgaaagg ggaatcgcat ctcgcggatt
     6121 ttccggctct gctggataac ccattaattc gtaatgggat catgcagtct cagcacttta
     6181 aaaccatctc tgcctactgg gataatctgg atgtcgccct ggtgggaatt ggctcaccgg
     6241 ccattcgcga cggcgctaac tggcatgcgt tttatggtgg tgaagagagt gacgacctga
     6301 atgcccgcca ggttgctggc gatatttgct cgcgcttttt tgatattcac ggcgcaatgg
     6361 ttgaaacgaa tatgagcgaa aaaacactct ctatcgaaat gaataaatta aagcaggcac
     6421 ggtattccat tggcattgcc atgagcgaag aaaaatacag cggaattgtt ggtgcactgc
     6481 gtggaaaata tattaattgt ctggtaacga atagcagcac agctgaacta ttactgaaat
     6541 aaaaatcttc ccgatgttta cc
//
LOCUS       DACADA010000215         6541 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.217, whole genome shotgun sequence.
ACCESSION   DACADA010000215 DACADA010000000
VERSION     DACADA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.217"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            128..1462
                     /locus_tag="ISB17_004377"
     CDS             128..1462
                     /locus_tag="ISB17_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1982239.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1515..1967
                     /locus_tag="ISB17_004378"
     CDS             1515..1967
                     /locus_tag="ISB17_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAP1982240.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2083..2406)
                     /locus_tag="ISB17_004379"
     CDS             complement(2083..2406)
                     /locus_tag="ISB17_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP1982241.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2390..2743)
                     /locus_tag="ISB17_004380"
     CDS             complement(2390..2743)
                     /locus_tag="ISB17_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1982242.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2977..4167
                     /gene="nepI"
                     /locus_tag="ISB17_004381"
     CDS             2977..4167
                     /gene="nepI"
                     /locus_tag="ISB17_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAP1982243.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4208..4501)
                     /locus_tag="ISB17_004382"
     CDS             complement(4208..4501)
                     /locus_tag="ISB17_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982244.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4723..5541
                     /gene="nlpA"
                     /locus_tag="ISB17_004383"
     CDS             4723..5541
                     /gene="nlpA"
                     /locus_tag="ISB17_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAP1982245.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5545..6468)
                     /locus_tag="ISB17_004384"
     CDS             complement(5545..6468)
                     /locus_tag="ISB17_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1982246.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1568 a         1479 c         1644 g         1850 t
ORIGIN      
        1 gaaacgtcgc gagattatta aagaatatcc attaatgtgc aattgaaatg tgataattat
       61 cactgaaccg ttgttgtcat ttgttttaca aaagcaaggg attaaatctc ctccaatgga
      121 caaaaaaatg aataatgaca ataccgatta cgtgagtaat gaatcaggga cgctttcgcg
      181 attatttaaa ctacctcagc atgggaccac cgtccgcaca gaattgattg cggggatgac
      241 cactttttta accatggtgt acatcgtttt tgtgaacccg caaatcctcg gcgcggcaca
      301 aatggaccca aaagtggtgt ttgttaccac ctgtttgatt gccggtatcg gcagtattgc
      361 gatgggggta tttgctaact tacccgtggc actggctccg gcaatgggcc tgaacgcctt
      421 ctttgccttc gtggtcgtgg gggcgatggg tatctcctgg cagaccggga tgggcgcgat
      481 attctggggc gcagttgggc tatttttgct cacgctgttt cgtatccggt actggatgat
      541 ctccaacatt ccattaagtt tacgtattgg tatcaccagc ggaatcggat tatttatcgc
      601 cttaatggga ttaaaaaata ctggcgttat tgtcgccaat aaagacaccc tggtgatgat
      661 tggcgattta agttctcacg gcgtgttgtt aggtatttta gggtttttta ttataactgt
      721 gttgtcatca cgtcattttc atgccgcggt gcttgtttct attgtggtga cgtcttgctg
      781 tggattattt ttcggtgatg ttcattttag cggtgtctat tccattccgc ctgatattag
      841 cggcgtcatt ggtgaagtgg atttgagcgg tgcgttatca cttgaactcg ccggtatcat
      901 tttctccttt atgctgatca acttatttga ttcatcaggg acattaattg gtgtgactga
      961 taaagctggc ttaatagata gtaacggtaa attccccaat atgaataagg cgctgtatgt
     1021 tgatagcatc agttcggtgg cgggtgcgtt tatcggtacc tcgtctgtta ccgcctatat
     1081 tgaaagtact tcaggtgtgg ctgtcggtgg tcgcacgggg ctgactgcgg ttgttgtcgg
     1141 cgttatgttt ctgctggtta tgttcttctc accgctggtg gcgatggttc ctccttacgc
     1201 aaccgccgga gcgttaatct ttgttggcgt attgatgaca tcgagcctgg cacgcgttaa
     1261 ctgggatgat tttaccgaat cggtgcctgc gtttattacc acggtgatga tgccctttac
     1321 gttctcgatc accgaaggga ttgcactagg ctttatgtcg tactgcatca tgaaagtgtg
     1381 caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc tgtttgcact
     1441 gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg agttctgatt
     1501 acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg tttgtggttg
     1561 gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc gatcggctga
     1621 acatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca gccggtgacg
     1681 aatttttacg ttatttgcat cgcccggatg agtcgcacct gcaaaacgcc gcgcaggtgt
     1741 tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca gaacctgctg caatggcatc
     1801 ggattttaca aaaagcccgc cttgccgcgc cgattaccga tgctcaggtc agactggcgc
     1861 tgggttttct gcgcgaaacc gaacctgaaa tgcaggatat caatgctttt cagatgcgct
     1921 ataacgcgtt ctttcagcct gccgagggcg ttcactggct gcattaaaac cgcttcgtct
     1981 tatcagccta cagaccgcgc ctgacctggt gggtggataa ggcgtgtacg tcgcatccgg
     2041 tatcggaatt gacagtaggt gaacgcatta tctacgttct atttacagca gcacctccag
     2101 cccgtgtttt tgcacaatgc tgagcagttt taatgaaatg ccattggggc gtttagcgcc
     2161 actttcccat ttttgtacag ttgaaacgct ggtattgaga tagtgagcga agacgggctg
     2221 actcactttt aatttttccc gcagtgcttt tatctcaaca ggttgtaagt cttgcacgct
     2281 ggtcaggcag gctttgtcaa aagtacgcat cgtttcggca gaaatagcat cgactctgga
     2341 caatccagcc gctgcgctgt gaatagcttc aaatgcagga cttttaaatt tagtttttgc
     2401 agtcatagca gatctctgtc agttctttag tgttaatcat tgctgtcagt tgggctttcg
     2461 tcaggaaggc ataatgcttt gcaagttcac gaaacccagc cagttcctga ctattaatgt
     2521 tcgacatatc ctgttttgca tataaaaacg tgtaaaacca attgcttccc cctttcgcca
     2581 gaataattgc acgctcacga tttcggtgaa ggcgcttttt aaaaacgcca ccaccaagta
     2641 catcagcttt accttgttcc gtttctaaaa tggcccggca caactcttca tctgttattg
     2701 catgagacct ggcttccctt gcaaaccatt tggttttaaa cacgcgcata tcgttgagat
     2761 cctgttatta aagtatatca ccaagtgcaa tactttatcc cttattccca acctaatcgc
     2821 gaaaatgcat atcaccttgc cctatgattt ttatttcttc gagcagcctc gaacttttcc
     2881 cacaacaccg taaaagttgc aaaaacgtta aatacgtcac acatttcaat gttaaagtgg
     2941 tcggctttcc ctctgaaaca tgccacgggt aacaccatga gtgaatttat tgctgaaaac
     3001 cgcggcgcgg atgccatcac ccgaccgaac tggtcagccg ttttctcggt ggcattttgc
     3061 gttgcctgtc ttattatcgt tgagtttttg cccgtcagtt tgttgacgcc aatggcccag
     3121 gatttaggca tttcggaagg ggttgccggg caatcggtga ccgtaaccgc ttttgtggcg
     3181 atgtttgcca gtttgtttat tacccagacc attcaggcca ctgaccgccg ctacgttgtc
     3241 attctgttcg ccgttttgct gacgctttcc tgtttgctgg tttcctttgc caactcattc
     3301 agtctgttgt taatcggtcg cgcctgtctg gggctggcgc tgggcggatt ctgggcgatg
     3361 tcggcgtcgc tgaccatgcg tctggtaccg ccgcgtacgg tgccgaaggc gctgtcggtg
     3421 atcttcggcg cggtttctat tgcgctggtg attgccgcgc cgttgggcag ttttttaggc
     3481 gagcttatcg gttggcgcaa tgtctttaat gcggcggcgg tgatgggcgt gctgtgtatt
     3541 ttctggatta tcaaatcact gccttcgctg ccaggcgaac cttcgcatca gaaacaaaat
     3601 actttccgct tattacaacg tccgggagtg atggccggga tgatcgccat ctttatgtcc
     3661 tttgccggac agtttgcttt tttcacttat attcgcccag tgtatatgaa tctggcggga
     3721 ttcggtgttg atggcttaac cctggtgctg ctgagttttg gtatcgccag ctttgtcggg
     3781 acatcgcttt cgtcgtttat tctcaaacgt tcggtgaaac tggccttagc gggcgcaccg
     3841 tttgtgctcg ccctgagcgc gctggtactg acgctgtggg gaagcgataa aattgttgct
     3901 actggtgtgg cgattatctg ggggctgacc tttgctttaa ttcccgttgg ctggtcaacg
     3961 tggatcactc gctcactggc cgatcaggca gaaaaagccg gatctattca ggtggccgtt
     4021 attcagcttg ccaatacctg cggtgcggca atcggcggtt atgcgttgga taacattggt
     4081 ctgacttcgc cgctgatgtt gtccggcaca ttgatgttgc tgactgcatt gttggtgact
     4141 gcaaaggtga aaatgaagaa atcctgatat caggtaaaga agcgttggtc agtggttgtt
     4201 gccgttctta catatccggg cattctacct tacccaacgc ttcccaataa ttgctctgat
     4261 tattacgttc catcgctacg atggcttcgc gtatgtgttg tttattgctt tcatcagaca
     4321 acattaactt ttcccacgct tcatctgata acgatgcctg cggggtacaa attttacgta
     4381 aatcttgcaa taccgtggtt ttctcttttg ccgactgtag ggaactgaat ggcgactcgg
     4441 cctgagaaat ggctggaaaa atcaggaata tggtaatcat gagtagcatc gttggcttca
     4501 tagcggcaac cttctgcaaa tgtgcatcga atgaagcata agtgtagttt gcttttgccg
     4561 ctctcatcat gctgtacgaa aggtgtttgc gtggtcagta agaagtgcca gactttatat
     4621 tccactttta ttccttttta ttcttatcga tagcgtttcg ttttttaaac tgcagcgacc
     4681 ttaccgctat agtcaggtaa tcattaataa aaggataaaa aaatgaaact gacaacacat
     4741 catctacggg cgggggccgc attattactg gcaggagttc tgctggcagg ttgcgaccag
     4801 agcagtagcg atgaaaagca tattaaagtt ggcgttatta atggcgcaga acaagatgtc
     4861 gcggaagtcg ctaaaaaggt agcaaaagag aaatatggcc tcgatgttga actggtagga
     4921 tttagcggtt cgttgctacc gaacgatgcg acaaatcatg gcgagcttga tgcgaacgtc
     4981 ttccagcatc gaccctttct tgaacaagat aatcaggcgc atggttataa actggtagcg
     5041 gtgggaaata cgtttgtctt ccccatggcg ggttattcca aaaaaatcaa aaccgtggcg
     5101 caaataaaag aaggcgcgac agtggcgatc cccaacgacc cgactaacct tggtcgtgcg
     5161 cttttactgc tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct
     5221 accgcgttgg atataaccga taatccacgc catttgcaga taatggagct ggaaggggca
     5281 cagttgccac gtgtactgga cgacccgaaa gtggatgtag cgattatcag caccacttac
     5341 atccagcaaa caggactttc tccggttaac gacagcgtat ttattgaaga taaaaattcg
     5401 ccgtatgtga atattctggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa
     5461 tttctgcaat cttatcaatc acccgaagtc gctaaagcgg cagaaaccat ctttaacgga
     5521 ggcgcggtgc ctggctggta attctcactc atgccgcgcc ggacgattta tttttctggc
     5581 gcggcggcgg gaatccattg aaatcaaaat caccgatgac agaatcagca gcgttcccag
     5641 ccagtccggc aatgtgaagg tgatccccag caacagcaaa gagagcagtg cgctacttaa
     5701 tggttctgca cagcttaaaa tgctggcttt tggaccacca attaattgtg ctcctttcag
     5761 gtacagacta aatgtcaggg acgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa
     5821 actgccgtta acgacaaagt ttgtcccttg tctggcataa aagggcagca ggatcagacc
     5881 gccaatgagc atactccagc cgacgaccgg taacgtgcca tagcgagcaa tcagcgttga
     5941 gggataggtg gtatagaatg cagcagcaaa tgccgaggca atgccccaga ataacgcggc
     6001 aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag tcccgatcag
     6061 cgatgtcaaa atagcgcaaa aaaccaggat gccggggcgg gatttacgca ccagtgagaa
     6121 ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc gttgccgtgg ctgcgttcga
     6181 tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca cggaaaaaat
     6241 cagtaggcta atggcatctt tgtgattgtt aataatagaa aagattttat caccatgaac
     6301 aaatgatagc gtcagcagaa tcaatccggc gaatatcaaa cgcgtcatag tcaaaaactg
     6361 cgacgacatc tggctttgct ccatgatgta ttgcgcgcag acccctgaac ttccccacaa
     6421 cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc
     6481 atactgtttt gtcttttcat caatatacgt taaggatacc actaccgatg acggtgaggg
     6541 a
//
LOCUS       DACADA010000216         6494 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.218, whole genome shotgun sequence.
ACCESSION   DACADA010000216 DACADA010000000
VERSION     DACADA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.218"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..647)
                     /locus_tag="ISB17_004385"
     CDS             complement(<1..647)
                     /locus_tag="ISB17_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP1982247.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDME"
     gene            complement(942..3260)
                     /gene="yicI"
                     /locus_tag="ISB17_004386"
     CDS             complement(942..3260)
                     /gene="yicI"
                     /locus_tag="ISB17_004386"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAP1982248.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3270..4652)
                     /locus_tag="ISB17_004387"
     CDS             complement(3270..4652)
                     /locus_tag="ISB17_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAP1982249.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4945..5039
                     /locus_tag="ISB17_004388"
     tRNA            4945..5039
                     /locus_tag="ISB17_004388"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4979..4981,aa:Sec,seq:tca)
     gene            5092..6129
                     /locus_tag="ISB17_004389"
     CDS             5092..6129
                     /locus_tag="ISB17_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HAP1982250.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
BASE COUNT         1770 a         1672 c         1470 g         1582 t
ORIGIN      
        1 ttttccatat ccagtaacaa tgactggaca aataacactt cttcctctcc aaaataatgg
       61 actaatgctt tatagcttcc gggatccaga cgtgaataca cgacaccttg cggctcatta
      121 tggttaataa tgccgttaat cagtaatgcc attgcctgtc ggacttgccc ttcactacct
      181 tcaatatgcg taccacttgg gctgcggatc aacgataacc ccaacggcgc aagccaggac
      241 tcaattactc tcaggtcatt aacaatagag gctccgctga taaaatagcg ttctgacaat
      301 ttactgatcg acgtttcatt cggtgtttca cttaataact gcgacgctat tttaacgcgc
      361 ctggcattat tcattacacc atcaatctct tcacattctg ccaccaacaa atgatcaaga
      421 tgcagcaaat cagcaatatt ctccgcgctt aacatcacac ccgcgccagc acgcttctca
      481 acatttattc tccattgtcc cagccattgc tcaagtaaac gtaaatcacg ttgaatggtt
      541 ttttccgaaa catttaaata gccggcaatc gtaacaatag tcgtatattc ccttcgaggt
      601 aaaaggaatc gtaataatct gttttgtcgc gaggtaatca tttgcatttt ttacccttat
      661 cacagattaa gaagccccat atggaagagc ttcagttgcc atctctgagg gtaaatataa
      721 agcagaataa tttcacaaca atactaaaat ctgtccgtaa tccggacagt gataaaacaa
      781 agcaaggcgg ttaagggata tttttcagca cagatcacat tttcgtgatg aataatcgtg
      841 atcatgctcg caaatacgat ggaagaaata tttattacca gatgtgagat tatttaccgg
      901 atacgacaca aaaccattcg tatccggcat tcttcaatag attacaacgt aattgtcagc
      961 gcattccctt gaggagtcac caccagtccc tgctcacttt cagcctgcga accgccttgc
     1021 agaccattta ctttcacaat attgcgcaag cacagtgtcc agttcttcgc ctcgcccgta
     1081 ccagtcacag taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgaaccg
     1141 tcagcagtgg gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat
     1201 gccgtacctt cgtgccacgc gtaatcggga cgttgatcgt tgttgcccag cgccagtagg
     1261 gtgctatcac gcacataaac gggcagactc aggaagctgt gctgctgttt atgccagcga
     1321 ctaccgtcga gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac
     1381 tgcacatcgc ccgcttcagt gaacacaggc gcaaccatca cgttgtcgcc taacatgtat
     1441 tgacggtcaa ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc
     1501 atcggcgtac cccgggcgtt tgcacgtgca gcttcacgat acagatacgg catcatgcgg
     1561 catttcagtt gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc
     1621 acacgataag atttgctgcc atgtaaacgg ctatggctgg agagcaaacc aaacgcgcac
     1681 cagcgtttgt aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag
     1741 aagccaaaac ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg
     1801 tagttagcgt aacagtcgcc accccagtgt accggaaatt tctgcgcacc aacggaggcc
     1861 gagcgggcaa acaagacggc ttcttcctca ccaacggtgt ccttgagcac gttccacacc
     1921 agttcgttgt agatgtatgc ataatggtta tgcattttct gcggatcgga accatcaaac
     1981 cattgaacat cggttggaat acgttcgcca aagtcggtct taaagcaatc aacgcccatc
     2041 gcgaccagac ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca
     2101 taaatcgcca gacctggctg ccatttatcc cactgccaca gcgaaccgtc cgggcgtttg
     2161 agtaaataac ctttctcttg taactcttta aagacggggg atttttgccc aatatagggg
     2221 ttaatccaga cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg
     2281 tccggaaaag tcagcggatc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa
     2341 cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg
     2401 tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg ttagccacag accgaaggac
     2461 cacgcgggcg gcagcgccgg acggccagta aaacgggtat aacgatcaag taccgcttta
     2521 ggcgtcgggc cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgtact
     2581 ttggagactt tctccgatcc cacttcaaaa gagacacatt ggggatgatt gaccagtacc
     2641 ccataaccac ggttagtcac ataaaacggg atatttttat acgcctgttc agtacttgtg
     2701 ccgccgtccc ggttccaggt ttctaccgtc tgaccattgc gcaccagggc agtaaagcgc
     2761 tctcccaggc cgtaaactgt ttcgccaacg ccaagatcca gccgttcaaa catataattt
     2821 cgttgattat tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt
     2881 tcgccgttgc gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcacttaag
     2941 ttgccacttt taaactcagc gtaacgttct gtgttttcga tagtgacctt cacgtcctgc
     3001 aaaatattga gcgggtaatg aggaccgtta ttcagcgccc cctgaaaatg ctcaatccgt
     3061 acaccgacaa taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc
     3121 tgccaggtac gttcacgcac atcacgaggg gcagcataga ccaccatttc attaccctgc
     3181 tgttcaacct cgaacacctg aagcgggtga atcaaattga ggccaggttg aatcaaccag
     3241 tttccgtcgc taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt
     3301 tgctggcaac ggcgtttgcc ctgcgccagc tgttccataa cggttttcag attgtgcgtc
     3361 gtgagcgagt agtagcgttt agcgataatc gcgctcagca agtaacagat cgccggaaca
     3421 atcgtgaata gcgcgataat gatgctaatt gtggcgctgt tctgcgcttt ttctgccgca
     3481 tcatatccgc cataagccag catccagccg ataagggcac caccgaaggc caaccccagt
     3541 ttgagcacaa acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attacaccac
     3601 tcgccgtagt cgacggtatc ggacatcatt acccactgga taggtgtcac cagttgatgc
     3661 aacacaccaa tcacgaagat gaagacaaac atggtgatgc tggcctgcat aggaacaaag
     3721 aacatcgcga ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta
     3781 catttccagt cggtcagcgg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa
     3841 gtggtgagaa aagcaacaaa tacttccggc gtacccaaaa tccatgtgac gtaatacatc
     3901 atcgccccac cacgtacgca caccgccagg atattgaaaa tggtcaggaa accgacaata
     3961 cgccactggt cgttttgcca gatatcacgt aaatcatccc gcatagacgt cgttgtaggt
     4021 ggtgcttcaa cgcgttcttt agtggtgaag aaacagaatg ccagcatcat aaatgccacc
     4081 acggaaagga ccgcgatacc gccctggaaa ccgagtggtt tattatcacc accaattaaa
     4141 ttaaccagtg gcatcatcag aacagtagag agcatgcctc ccgcggtcgc cagcacaaaa
     4201 cgccaggatt gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg
     4261 cagtaaggga tattgacgat ggtataaagt aaggtaagta gggtgtaagt aattgctgca
     4321 taaatcattt ttccgttcat actgagatct ggcgtgctat aggctagtac acagacgatc
     4381 ccgaatggca gtgcaccaaa cagtacccac ggccgaaatt taccccagcg agagcgcgtt
     4441 cgatcggcca acaatcccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
     4501 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
     4561 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
     4621 ttctctttaa cggacaacac ttcactcttc atctgctttt ccttgatgct ataggggtgc
     4681 tgagaccgat aacgcatttt gtaaaccatc cctgcgagag cgaatattcc gatatctggt
     4741 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaatttcgc tcatatatca
     4801 ttgtaaaata tgagttttat atgaactata atgctttcgt gataatacgc tgcgtgtatt
     4861 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
     4921 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
     4981 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccaa
     5041 aatgcctctc ctgaactctc ccgacgtcaa caacactcct tcggaggcaa catgtcagac
     5101 aaagacttaa cccagccccc agcaggcgag tttattatgt ttgccagcgg tgacggaaga
     5161 gtgcgtgttg aatgtcgctt tgagtcagac acaatctggt tatcccaggc ggcaatggct
     5221 gaactgtacg ataaagatgt tcgcactatt aacgaacatt taatcaatat attttccgaa
     5281 ggtgaacttg ttcaaaactc aaccatccgg aaattccgga tagttcgcca ggaaggaaaa
     5341 cgtcaggttt ccagagagat agaccattac aacctcgaag ccattctcgc tgtcggctat
     5401 cgcgttcgct cgcctcgagg cacgcaattc cgccagtggg ccacccagac gcttcaggaa
     5461 tacctgatca aaggttttgt gatggacgat gagcggctga agaatccgcc tgtcggttca
     5521 tcggttgtgc ccgactattt tgatgagatg cttgaacgca tccgcgatat tcgcgccagc
     5581 gagcgtcggg tatatttgcg ggtgcgggag atctttgctt tagctgccga ctatcagcca
     5641 tcgcttaaag aaaccacgca attttttcaa accatccaga ataaactgca ttttgcctgt
     5701 accgggcata ccgctgcgga actcatccac aaacgagcag atgccagcca gccgcacatg
     5761 ggcttgacca gttataaagg tgaagaggta cgtaagggcg acgtgacggt ggcaaaaaat
     5821 tatctcaccc aggatgaggt cagcgaactt aatcgcgtgg ttaatatgtg gctggacttt
     5881 gccgaggatc aggcccgtcg tcgtcagcaa gtctttttgc gcgactggca ggataagctg
     5941 gatcagttcc tgcaatttaa cgaccgagag gttctacagg gcgctggtaa aatcagcaag
     6001 aaaatggccg atgaaaaagc gcaggcggag tatgttcagt ttgccgaaca acaacggcgc
     6061 ttaaaagaag ccgaaggcga gaaggatatc gctggtttgc tacagtggaa taaagagtcg
     6121 aaaaagtagc ccggaatccc cccaaacgcc atacgcaaaa ccatttgggg gtacttatgg
     6181 ggttactcat ttatcaagtt caaaatataa acattaaatc aataacttaa ccatcaaatg
     6241 cgactcctgt gatcgccaaa atgcctcttc agtgatcccc atcctgctta tttctgagca
     6301 acctgaccct gctgatgtat ctggtagtat gacagagtga ttcatttttc ttgccgagtg
     6361 tacgaaaacg tcttccttgc caccgaccat caaccgtcac tcaaaaaaac ctctcttttt
     6421 tcaagctatc aagaataagt tgcattttat ctgcaccgga caccagccat ccatatatgg
     6481 cttttacctg ctac
//
LOCUS       DACADA010000217         6462 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.219, whole genome shotgun sequence.
ACCESSION   DACADA010000217 DACADA010000000
VERSION     DACADA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.219"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(311..1981)
                     /gene="betA"
                     /locus_tag="ISB17_004390"
     CDS             complement(311..1981)
                     /gene="betA"
                     /locus_tag="ISB17_004390"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HAP1982251.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFNIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1995..3467)
                     /gene="betB"
                     /locus_tag="ISB17_004391"
     CDS             complement(1995..3467)
                     /gene="betB"
                     /locus_tag="ISB17_004391"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HAP1982252.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3481..4068)
                     /gene="betI"
                     /locus_tag="ISB17_004392"
     CDS             complement(3481..4068)
                     /gene="betI"
                     /locus_tag="ISB17_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HAP1982253.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4197..6230
                     /gene="betT"
                     /locus_tag="ISB17_004393"
     CDS             4197..6230
                     /gene="betT"
                     /locus_tag="ISB17_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HAP1982254.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1346 a         1754 c         1769 g         1593 t
ORIGIN      
        1 ctgacttatt tttgtactcg ttacaagaac ccttttcgta tatgctaacc gtgagcggga
       61 atgaacaggt aaccatagcc aggaacatta gcggcgtaat tcttaagctg agtggtattt
      121 catctgtagt ttatctgtgt atgcatagca cattcatcag ccagattgag caagtcagct
      181 tagaatttgc tggcgagatt gtcacgatga agcatgagag ttacctcagt gttttatata
      241 aggattcggt cctcatagta gaacggtaac tctcaatctg ataggttcct gagtcagttc
      301 acatcacgac tcattttttc gctctcaccg gcatcccatt tgccacaaaa tatcccgccg
      361 tgctcctcgg caacgcttcc ttcccacgaa tcatatccgc tattttctcg ccaatcataa
      421 tcgtcgtggc gttcaaattg ccggtgataa tttgcggcat aattgacgca tccaccacgc
      481 gcaggccttc cagcccgtgt acgcggcctt cgccgtcaac cacagccatc tcgtcataac
      541 ccattttgca ggtaccgcac ggatgaaacg cggtttcggc gtggttacgc acgaactcat
      601 cgagctgctc atccgtctgg cattcgacac cagggctgat ttcgcggcca cggtattgat
      661 ccagcgcggg ttgatgcatg atctcgcgtg tgatgcgaat tgcgtcgcgg aactcctgcc
      721 agtcctgctc gtgcgacatg tagttaaaca gaattcccgg atgctggtgc gggtcgcggg
      781 atttaatccg cacatgccca cggcttggcg agcgcatcga tccgacatgg cattggaagc
      841 cgtgctcttt cactgcgttc gagccgttat aattaatcgc cactggcagg aaatggtact
      901 gaatattcgg ccacgcaaat tcctcacggc tgcgaataaa tccgcctgct tcaaagtggt
      961 tgctggcacc aacgccggtg ccgccaaaca gccactccgc accgattctc ggctgattcc
     1021 accactgcag ggcagggtag agggaaaccg gttctttgca ctcgtattgc agatacatct
     1081 ccaggtgatc ctgaagattt tcgccgacgc cgggtaattc atgcaccagc ggaatattaa
     1141 actccgccag cagttcagcg ttgccgacgc cggagcgttg caggatctgc ggtgaagcaa
     1201 tcgcgcctgc acataacagc acttctttat tcgccgccgc gcgggtcgga atagtgctgt
     1261 cgccttccag ccactcgacg cccaccgcgc gtttgccgtc aaaaatgatg tgatcggtca
     1321 tggcgtgagt acgaatggtc aggttagggc gcgatttggc ctgatcgaga tagccacgcg
     1381 cggtgctggc gcgacggcca tgcggcgtga cggtgcgatc catcgggcca aaaccttctt
     1441 gctgatagcc gttgagatcg tccgtgcgtg ggtagcctgc ctgcatgccc gcttcaatca
     1501 tcgcttcaaa cagcggattg acgccgggtt tggaggtgat gacgctcacc gggccatcgc
     1561 caccgtggta atcgttctcg cccacatcgc gagtctcggc cttgcggtag tagggcaggc
     1621 agtcgagata actccagttc tccagaccag gttcttgtgc ccagttatcg agatccagcg
     1681 cattgccacg gatgtagcac ataccgttga tcagcgatga accgcccaga cctttgccgc
     1741 gtccgcactc catgcggcgg ttgttcataa acggttcggg ttccgtctca taggcccagt
     1801 tgtagcgttt gccctgtagc gggaacgcca gcgcggcggg catctgggtg cggaagtcaa
     1861 agcgatagtc cgggccgcct gcttcaagca gcagcacggt tgtgttcgga tcttcagtca
     1921 gacgggtagc gagaacgttg ccggctgagc cggcaccaat aatgatgtag tcaaattgca
     1981 aataaacctc ctggttagaa tatggactgg aatttagcca tctcaacctg gatggacttc
     2041 acctgggtgt aactctggag cgtcatcacg ccgttctcgc gaccaatgcc ggagtgtttg
     2101 tagccgccaa cgggcatctc tgccggggat tcgccccagg tgttgatcca gcaaataccc
     2161 gcttccagct gatgaatgac gcgatgcgcg aggttcaggt ccgccgtcac gatacccgcc
     2221 gccagaccgt agtcggtatc gttagcgcgg cgaatgactt cgtcttccga ctcgtagatc
     2281 agaatggaca tcactggccc gaaaatctct tctcgcacaa tggtcatatc gtcgctgcaa
     2341 tcggtgaaca cggtcggtgc aacccatgcg ccgttatcga agctatcccc cttcagtaca
     2401 ttgccgccgc acagtacgcg cgcgccttcc tctttgcctt tggcgatata gcgcagcacg
     2461 ttatcgcgat gcgggaagct gaccagcggg ccgaagttag tttgcggatc gaaaacgtcg
     2521 cccgcgcgaa tgcgctcaac gcgcgccaga accttttgtt caaatgcggc tttgcatttc
     2581 gtcggaacga agacgcgagt gccgttggta cacacctgac cggagctgaa gaaattcgcc
     2641 atcatggcga tatcggcggc gagatcgaga tccgcatcgt cgaaaacgat cagcggtgat
     2701 ttaccgccca gctccatggt cacttctttc agggaggaag ctgccgagtt agccatcact
     2761 tttttgccgc tggcgacacc gccggtaaat gacactttgg caatgcccgg atgatcggtc
     2821 agatattgtc cggtctccgc gcccacgccc ggcaacacgt taaatacgcc gtccggcagg
     2881 cccgcttcgc tgtaaatttc ggccagcttt aacgcggtaa gcggggtgac ttcgctcggt
     2941 ttgaaaatca ttgcattgcc tgccgccagc gctggggcgg atttccacaa agcaatctgg
     3001 atcgggtagt tccatgcgcc aatccccgcc accacaccca gcggttcgcg gcgggtataa
     3061 acaaatgacg tctcacgcaa cgggatctgg ctgccttcca gcgccgggat cagcccggca
     3121 tagtactcca gcacgtccgc accggtaacg atatcgacgg ttgaggtttc cgaatatgct
     3181 tttccggtgt caagggtttc cagttttgcg agttcgtcat tgcgttcacg cagaatatca
     3241 acggcccgac gcagaatacg cgagcgctcc atagcggtca tcgccgccca gattttttgc
     3301 ccttgttggg cactcttcac ggcgcgatcg acatcctcac gcccggcggc ctgcacggtc
     3361 gccagcacgt taccgttggc cgggttaatg gtctcgaagg tgcgaccgct ggtggcggag
     3421 gtataaccac catgtatata aagctgctgt tctgccattc gggacatcac gtctcctcgg
     3481 ttaatcggtg ggtaaatgct gagtaataaa gtgacgggtc agcgagtgtg cgagaggttt
     3541 atccagcggt ttgccgctca gtgccgcgcg cagccataat ccgtcaatta gcgcggcaag
     3601 gccgtagccc gcttcctgtg cctgttggcg cggtaactcg cggcgaaatt cgctcaccag
     3661 attcgacaat aagcggcggc tactgacctg ctgcaaacgg taaagcatcg gctgatgcat
     3721 actgctggcc cagaaggcca gccaggcttt catcgccgca ctgctcacct gggtttcatc
     3781 gaagtttccg ccaacaatcg cctgtaatcg cagctctgca ctgccctgcg gaagtgcatg
     3841 taatcgattc aaaaccgcgt cacgcagctg actggtgata tcgcgcatgg ttgcttccag
     3901 cagaccattt ttgtccctga aatagtggct gatgatcccc gtagaaacgc ctgcacggcg
     3961 ggcgatctgc gcgatcgttg catcgtgcat gcccacttca tttattgctt ccagcgtggc
     4021 gtcgatcagt tgtctgcgcc ggatcgactg cattcccaat ttgggcattt tcgccactcc
     4081 attcatcagc gttgtttatc tattaaagcg gttattgatt ggacgttcaa tataaaatgt
     4141 gtcttaattg ttactaattt gattttaaat ggtaacaata acagtgggga tactggatga
     4201 cagacctttc acacagcagg gaaaaggaca aaatcaatcc ggtggtgttt tacacttccg
     4261 ccggactgat tttgttgttt tccctgacaa cgatcctgtt tcgcgacttc tcggccctgt
     4321 ggattggccg cacgctggac tgggtttcta aaaccttcgg ttggtactat ctgctggcgg
     4381 caacgctcta tattgtcttt gtggtctgta tcgcttgttc gcgttttggt tcggtgaagc
     4441 tcgggccaga acaatcaaaa ccggaattca gtttgctgag ttgggcggcg atgctgtttg
     4501 ctgccgggat cggtatcgac ctgatgttct tctccgtagc cgaaccggta acgcagtata
     4561 tgcagccgcc ggaaggcgcg ggacagacga ttgaggccgc gcgtcaggcg atggtctgga
     4621 cgctgtttca ctacggctta accggctggt cgatgtatgc gctgatgggc atggcgctcg
     4681 gatactttag ctatcgttat aatttgccgc tcaccatccg ctcggcgctg tacccgatct
     4741 tcggtaaacg gattaacggg ccgataggtc actcagtgga tattgctgcg gtgatcggca
     4801 ccatcttcgg tattgccact acgctcggta tcggtgtggt gcagcttaac tatggcttga
     4861 gcgtgctgtt tgatattccc gattcgatgg cggcgaaagc ggcactgatc gccttgtcgg
     4921 tgatcatcgc cactatctcg gtgacatccg gtgtcgataa gggcattcgc gtgttatcgg
     4981 aactcaacgt tgcgctggcg ctgggattga ttctgttcgt gttgtttatg ggcgacacct
     5041 cgttcctgct taatgcactg gtgctgaacg ttggcgacta tgtgaatcgc tttatgggca
     5101 tgacgctcaa tagttttgcc ttcgaccgtc cggttgagtg gatgaataac tggacgctct
     5161 tcttctgggc atggtgggtg gcatggtcgc cgtttgtcgg cttgttcctg gcgcgtatct
     5221 cgcgtgggcg taccattcgc cagttcgtgc tgggcacgtt gattattccg tttaccttca
     5281 cgctgttatg gctctcggta ttcggcaata gcgcgctgta tgaaatcatc cacggcggcg
     5341 cggcatttgc cgaggaagcg atggtccatc cggagcgcgg cttctacagc ctgctggcgc
     5401 agtatccggc gtttaccttt agcgcctccg tcgccaccat tactggcctg ctattttatg
     5461 tgacctcggc ggactccggt gcgctggtgc tggggaattt cacctcgcag cttaaagata
     5521 tcaacagcga cgcacccggc tggctgcgcg tcttctggtc ggtggcgatt ggcctgctga
     5581 cgctaggcat gctgatgacc aacgggatat ccgcgctgca aaacaccacg gtgattatgg
     5641 gcctgccgtt cagctttgtg atcttcttcg tgatggcggg gttgtataaa tctctgaagg
     5701 tagaagatta ccgccgtgaa agtgccaacc gcgataccgc accgcgaccg ctggggcttc
     5761 aggatcgcct gagctggaaa aaacgtctct cgcgcctgat gaattatccg ggcacgcgtt
     5821 acactaaaca gatgatggag acggtctgtt atccggcaat ggaagaagtg gcgcaggaac
     5881 tgcggttgcg cggcgcgtac gtggagctaa aaaacctgcc gccggaagag ggacaacagt
     5941 tggggcatct ggatttgttg gtgcatatgg gcgaagagca aaactttgtc tatcagattt
     6001 ggccacagca atattcggtg ccgggcttta cctaccgcgc acgtagcggg aaatcgacct
     6061 actaccggct ggaaaccttc ctgttagaag gcagccaggg caacgacctg atggactaca
     6121 gcaaagagca ggtgatcacc gatattctcg accagtacga gcggcacctt aactttatcc
     6181 atctccatcg tgaagcgccg ggccatagcg tgatgttccc ggacgcgtga ttgttattgc
     6241 ataaaaccgc gccatgtctg catatggcgc ggtttctctt acctcttgat acgttatatc
     6301 tgtgtacagt taagccctta gtatcgattg atgattgcca ggcaacagat aataaaaaag
     6361 aaaagaacta ttgaagccca aaacctacgc ttggactgtt gtgaatgttc aataagctta
     6421 gtcttattta atgtaaatat tggtgctaat ttgaaatgac gc
//
LOCUS       DACADA010000218         6452 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.220, whole genome shotgun sequence.
ACCESSION   DACADA010000218 DACADA010000000
VERSION     DACADA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.220"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            208..1302
                     /gene="ddlA"
                     /locus_tag="ISB17_004394"
     CDS             208..1302
                     /gene="ddlA"
                     /locus_tag="ISB17_004394"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP1982255.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1326..1538)
                     /locus_tag="ISB17_004395"
     CDS             complement(1326..1538)
                     /locus_tag="ISB17_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAP1982256.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1798..2106
                     /locus_tag="ISB17_004396"
     CDS             1798..2106
                     /locus_tag="ISB17_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAP1982257.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2165..3259)
                     /gene="yaiW"
                     /locus_tag="ISB17_004397"
     CDS             complement(2165..3259)
                     /gene="yaiW"
                     /locus_tag="ISB17_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAP1982258.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3272..4492)
                     /gene="sbmA"
                     /locus_tag="ISB17_004398"
     CDS             complement(3272..4492)
                     /gene="sbmA"
                     /locus_tag="ISB17_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAP1982259.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4844..6001
                     /gene="ampH"
                     /locus_tag="ISB17_004399"
     CDS             4844..6001
                     /gene="ampH"
                     /locus_tag="ISB17_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAP1982260.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6002..>6452)
                     /locus_tag="ISB17_004400"
     CDS             complement(6002..>6452)
                     /locus_tag="ISB17_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1982261.1"
                     /translation="NVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1625 a         1670 c         1564 g         1593 t
ORIGIN      
        1 cacatggtca tttaagccag gaataaatta gcttcccgtt catcattttt gtgatcttgc
       61 acacatcttt gagataacgc actttcttca ccagtacgtt gctcgctttg tcagcagaag
      121 attgcaacat tctgtcagaa ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact
      181 ttgtgttaga cgggataggt ttttaagatg gaaaaactgc gggtaggaat cgtttttggt
      241 ggtaaatcag cggaacatga agtgtctctg caatcggcaa aaaacattgt cgatgccatt
      301 gataaaagtc gcttcgatgt tgtgctgctg ggcattgata aacaagggca atggcacgtc
      361 agcgatgcca gcaattatct gctaaatgca gacgatcctg cccatattgc gttgcgtcct
      421 tcggcgacca gccttgcgca ggtgccaggt aaacatgagc atcagcttat cgacgcacaa
      481 aacggtcagc cgttgccgac ggtggatgtc attttcccga ttgtccacgg tacgctgggc
      541 gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt aggttctgat
      601 gttctggctt cagcggcctg tatggataaa gatgtcacca aacgtctgct gcgcgatgcc
      661 gggctgaaca ttgcgccatt tattaccctg acgcgcgcta atcgtcacaa catcagtttt
      721 gccgaagtgg agtctaaact ggggttaccg ctgtttgtaa aaccggctaa tcagggctct
      781 tctgttggtg tcagcaaagt aaccagtgaa gaacagtaca caattgccgt cgatctggcg
      841 ttcgagttcg atcataaagt gatcgttgag caagggatca aaggtcgtga aatcgaatgc
      901 gcagttcttg gcaacaataa cccgcaagca agcacctgtg gcgagatcgt actcaccagc
      961 gatttctatg cctacgacac caagtacatt gacgaagatg gcgcgaaagt ggtagttccg
     1021 gcagccattg cgccagaaat caacgataag atccgggcga ttgccgttca ggcttatcag
     1081 acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg
     1141 gtgatcaacg agatcaacac cctgcctggc ttcaccaaca tcagtatgta tccgaagctg
     1201 tggcaagcca gcggtctggg ttacaccgac ctgatcacgc gtttgattga gctggcgctg
     1261 gagcgtcacg ctgcggataa cgcactgaaa accacaatgt aatattcaaa cgcccggtaa
     1321 tattcttatt gggcgttttc agtttcttca tcgcgacgac gaataatcaa tcccgccagc
     1381 cagaaactaa tcacccacgt caccagaccg acggcatagg tctgccaacc ttttgcttca
     1441 aatcccagta accccaccac gccattaaga atgaatataa ggccaatggc gaacgcatag
     1501 tagtgccagt cacggcggat ttttacaggc agattcatgg cagctccgtt aacgaaaaac
     1561 aacatctttt catattctgt aacaactgtc tgtggtgtag acaacacttt cgcaattgtt
     1621 aataaattta acttaaggtt gctattttgt gactcgcaac gaagattcac gcttcgggaa
     1681 tattcccaac atcaatttac catgattctg atattgacaa tctaaatgac ctgtcagact
     1741 tgccataagc cgctggtatc acaccagaag agaagcagat gacttccgga ggtttttatg
     1801 gctgatttca ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg
     1861 cctggcaata ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg ggcacaattg
     1921 ctgaacctgc tagtgcatgc gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca
     1981 ggtcgcccac gggtggaaat tggtttaggt gtcggcacca tttttgggct gatcccgttt
     2041 ttagtaggct gcctcatttt tgcagtagtg gcgctatggc tgcactggcg acatcgccgc
     2101 cagtaagatt tacgttgcca gatgcggcct aaaacgcctt atctggcgtg acattcctgt
     2161 ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg
     2221 tagtcagatt gcgggtgatt ttcgggcttt ccagttgaat gccaggcaac atttcgcggg
     2281 gtagtgattt accggttttc gcttcggcaa gttgataaac tttcttgtac agcgccgtct
     2341 cctcaaaaga gaagctgtcc cctttctcta actgacggcg aatttcgctg tcactcatcc
     2401 ccagttttcc cgccagtttg cgcgttgcca gttcggtttt cccgggctct ttactgtcgt
     2461 aacgaattaa atcaccgtcc agtgccagct tcacgccgct ggccttactg accgcgttct
     2521 gaaacgcggc attacggctg gcgtaccagc cagcgttaaa atcagcaaaa cggtatatcg
     2581 gcgcgctata gctggcggga taattcagta aatggtaagt accaaaccac aacccgccgc
     2641 ggcgactgaa gacttcctga cggactgtac cgtccatttt ccacggatac cctttggtat
     2701 gctgttcggc aaaagcaata ctgacctgca tcggcccacc ggtgcgcacc ggattgagcg
     2761 aaccaaacag cgtctgcccc attggcacca tgctgatcag atcgtcaaaa atcgcgctca
     2821 attgcttttc tgtacgcacc gaatctaacc gttcgctata gcttttgccg tttggcgatt
     2881 tgatttttag cgcggtatgc accagaaaag caggaatgtg catccgttca gcacgacggt
     2941 caatctcttg ccaggcgatc ttgcttaagc ccggaacggc cggatctgcc tgataatttg
     3001 actcctgctg cgccaccgcc agcaccgagc agacgttttc cagcgtcggt gccagcccct
     3061 gactttcaaa ggtggtggcg agatctttcg cccaggcatc gcggtctttc acgctcgcag
     3121 gcatcttctg ccgcacgacg ctcgccacat ctatcgcttt ttcgcccttt ttcaacggtt
     3181 gcggagcctg actactacac cccgctaaaa ccagaaccgc caacaatgac agcgaagata
     3241 gaggattgac gcgcgacatt acttctcctt tttagctcaa ggtatgggtt acttcctgaa
     3301 ttttgtcacc atccagctca tgttcaaagc tgcgcagacg tttgtagata gacatcaact
     3361 caaccagtgt ggtccatgag ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa
     3421 cgttagtaat ctgcgtcatc aggccgagtg taatcgtacc ggcaacaatt gacggaaaca
     3481 gcaagaacaa accgaaaacg ttatcgacct gcaaatagag aatgcgggcg atattgaaat
     3541 acatatagtg aaaatagagg cggaaatagt ttttccgtac ggcgctaaaa agctcgcgta
     3601 ccgtaggcgg cgtcgcgcgc gtggcatcgt cttcaccata aaccagttct ttacggtagg
     3661 cagcctctac acgctggttt ttaaactcca gccccggcag tttgatccct accactgcca
     3721 gtaatccggt ccccatcagc gaccagacga tagcggcaat caccagacca tacggaatgt
     3781 gcccgacaat cggcagctcc ggcacatgtg cggagagcgt taccagcacc ggcaggaagg
     3841 cgatcaacgt catgatagcg ttgataaagc tgacgcccat attctccagc gttgaagcaa
     3901 aacgcatggt gtcttcctgc acacgctgcg cggccccttc gatatgacgc agttgttgcc
     3961 agttcgccat gtaatattcg ttcatcgccg tacgccagcg gaacacgtag tgactgacaa
     4021 agaagttgtt cagcacactg atcaccaccg cgatcagcgc aatccccaga aagacgccca
     4081 cttcgcggta aaattgttcg atggtgactt tatgcggcga acttagcgcc gtttgaatca
     4141 gatcatagaa cggcgcatac caggcgttga cggcaacccc gacttccacc aaaaaccagg
     4201 tgacgaagat aatcagcgca gtaccgagta ttgaccagta ttgccaacgg tgcgggctat
     4261 agataaacca gaacaatgca aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca
     4321 ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga
     4381 tacgcgccac ccaatcaccc ccaccgactt gccagaagat aacggcaatc aatgcccaaa
     4441 caaaggccga gagaaaaaac attcccggct ttgggaaaaa agacttaaac atatgcactc
     4501 ctgctaactt cttatcgttt taacaactgc tgtgtattaa ccgcatgacc gctacaaagc
     4561 gtcagccaaa tgaaaccgcc cattgtgacc catagcaagg cgcttagttc gccagcctga
     4621 ccataagaat tgtttcgacg ggtttcacaa accaaagatt gtttacataa gcggattaac
     4681 acgcgcagga tcacaaatgc aggcattggt gagcaggaaa aagatcgtgt ttacgcgccg
     4741 ttacgttttt aacactatta ttgcactaat tcgccctttg caatccatca atgagtagta
     4801 taaatacgct cagttacctt cattcaatct atggacacca ccgttgaaac gtagtctgct
     4861 tttttctgcc gtgctgtatt cggcgtcatt gacctccgtc catgcggctc agccgatcac
     4921 tgaaccggag tttgcctctg atattgtcga tcgttatgcc gatcatattt tttacggcag
     4981 cggtgccacg gggatggcgc tggttgttat cgacggtaac cagcgcgtct ttcgcagtta
     5041 tggcgaaacg cgacctggta ataacgttcg cccgcagctg gattccgtca tacgtattgc
     5101 ttccctcacc aagttaatga ccagtgagat gctggtaaaa ttgctcgacc aggggaccgt
     5161 gaagttaaac gatccgctaa gtaaatatgc tccgccaggc gcacgcgtac cgacctacaa
     5221 tgggacgccc attacgctgg tgaatctggc aacccatacc agcgccctgc cccgtgaaca
     5281 gcccggtggc gcggcacatc gtccggtatt tgtctggcca acgcgcgagc aacgctggaa
     5341 atacctttcc actgccaaac taaaagccgc gccgggcagc caggccgcgt actccaacct
     5401 cgcattcgat ctgctggcag atgcactggc gaatgcgtcc ggcaagcctt atacccagtt
     5461 gtttgaagag cagattaccc gtccgttggg gatgaaagac accacctaca ccccctcacc
     5521 ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc agtccgtgca ataacacgct
     5581 ggcggcaatt ggcagcggcg gcgtctattc cacgccaggc gatatgatgc gctggatgca
     5641 gcagtatttg tcatctgatt tttatcaacg cagtaaccag gctgatcgca tgcagacgtt
     5701 gatttatcag cgcgcgcagt ttacaaaagt gattggcatg gatgtccccg gcaaagcgga
     5761 tgcgcttggt ttaggctggg tatatatggc cccgaaagag ggtcgtccgg ggattattca
     5821 gaagacaggt ggtggtggcg gtttcattac ctatatggcg atgatcccgc agaagaatat
     5881 tggtgcgttt gtggtggtca ctcgttcgcc gttgacgcgc tttaaaaata tgagcgatgg
     5941 catcaatgac ctggtgaccg agctaagcgg gaataaaccg ttggttatcc ccgcatcctg
     6001 attaatactc tgaaggcaaa cggttgatag cgaccagttt gcctttattc atttcgatat
     6061 agccgccttt acgcaaagca gcgagaactt ccgcaacgac agaacgcgat atacgtgtac
     6121 gttgatggat ataattcatc acgccaatac gtgatcgcaa ttcttcattc cagtcaatca
     6181 ttgataataa cgtcgcgcgg atttgttcgt aggaattatg cccaatgagc tgaacgtcgc
     6241 gtaattccag aattctattt tgccaggcta accagtaaaa agcgtctcgc cagagttgat
     6301 tttgttcaat aagagtaatg gtttgtttgg ctggcaaatg atatcccgta cagtttcctt
     6361 ctgatattaa tttgtatggt atatcgtttt tcattaaacc atcagccagc cccataatat
     6421 aaggagcctg tgtaataccg ataagtacgt tt
//
LOCUS       DACADA010000219         6447 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.221, whole genome shotgun sequence.
ACCESSION   DACADA010000219 DACADA010000000
VERSION     DACADA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.221"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            229..768
                     /locus_tag="ISB17_004401"
     CDS             229..768
                     /locus_tag="ISB17_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1982262.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            831..1064
                     /locus_tag="ISB17_004402"
     CDS             831..1064
                     /locus_tag="ISB17_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1982263.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1130..3088
                     /locus_tag="ISB17_004403"
     CDS             1130..3088
                     /locus_tag="ISB17_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1982264.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3143..3577
                     /gene="psiB"
                     /locus_tag="ISB17_004404"
     CDS             3143..3577
                     /gene="psiB"
                     /locus_tag="ISB17_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1982265.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3574..4293
                     /locus_tag="ISB17_004405"
     CDS             3574..4293
                     /locus_tag="ISB17_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1982266.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            4539..5908
                     /locus_tag="ISB17_004406"
     CDS             join(4539..5019,5019..5908)
                     /locus_tag="ISB17_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HAP1982267.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            6019..6177
                     /locus_tag="ISB17_004407"
     CDS             6019..6177
                     /locus_tag="ISB17_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1982268.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1565 a         1591 c         1909 g         1382 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc atgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaaaaga tgatgggggc tgaaaacatc aacagaagga ggcacatcat ggcagttcgt
      241 ggcattaaca aggtcattct cgtaggacgc ctgggaaaag atccggaagt ccgttacatc
      301 cccaacgggg gcgcagtggc aaacctgcag gtcgccacgt cggaaacctg gcgtgacaaa
      361 cagacagggg agatgcggga gcagacagaa tggcatcgcg tggtgctgtt cggcaagctc
      421 gcggaagtgg ctggcgaata tctgcgcaag ggcgcgcagg tctacatcga aggtcagctt
      481 cgcacccgta gctgggaaga taacggtatc acccgttacg tcaccgaaat tcttgttaag
      541 accacaggca ccatgcagat gctgggacgt gccgcaggtg ctcagactca gccggaagag
      601 gggcaacagt tcagcggtca gcctcagccg gaaccacagt cggaggccgg tacgaaaaaa
      661 ggtggcgcaa aaacgaaagg ccgtggacgt aaggccgcgc agcccgagcc tcagccgcaa
      721 ccgccggagg gggaggatta cgggttttca gacgatatcc cgttctgatc gggctgactg
      781 tgataaccgc cccgccccgt tctgtgcggg gcatcaccgg agagatgaga atgagcgaat
      841 atttcagaat acttcagggg ctgcctgacg gtccttttac ccgcaaacat gccgaagctg
      901 ttgccgcaca gtaccggaac gtctttatcg aggatgacca cggcgaacag tttcgcctgg
      961 ttgtccgtaa taacggtgca atggtctggc gtacctggaa ttttgaggac ggtgccgggt
     1021 actggatgaa ccatgtcatc agggatttcg ggattcttaa gtaagagagg tgccggacgg
     1081 ggacacgtcc ggcagacaat cattattacc gttttgggag gtactaacta tgtcagttac
     1141 agagtctaag gcaaaaacgg agcgtaaatc cagccgtaaa cctgcaaaaa cgcaggaaac
     1201 agtcctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg cctcactgat
     1261 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgtg gagtccgtca gcgaactggc
     1321 tgagtccatt aagggcgttg gcctgttgca gaatctggtc gtgcataccc tgcctggtga
     1381 ccgttacggt gtcgccgcag gtggtcgccg actggctgca ctcaacatgc tggcagagcg
     1441 caacatcctt ccggctgact ggcctgtacg cgtgaaggtt attctgcagg aactggcgac
     1501 tgccgcatca atgactgaaa acggtcatcg tcgggatatg caccctgccg aacagattgc
     1561 cggattccgc gcaatggcgc aggaaggcaa aacacctgcg cagactggcg acctgctggg
     1621 ctattcaccc cgccacgtcc agcgaatgct gaaactggca gaccttgcac ccgtcatcct
     1681 tgatgcgctg gcagaagacc gcatcaccac cgaacactgt caggcgctgg cgctggagaa
     1741 cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgc cagtcaggat ggggcggtaa
     1801 accggatgtg cgggttatcc gcaacctgat taccgaaagt gaagtggcgg tggctgggaa
     1861 cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca gacgaactgc gcaccgattt
     1921 gttcagcgac gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg ccctgctgga
     1981 aaaactccag gctgtcgcag agttccttcg ggaagccgaa ggctggggat ggtgtgccgg
     2041 acgcatggag cctgtaggtg agtgccgtga ggatgccgga acataccgct gtctgccgga
     2101 gccggaagcg gtgctgacgg aggcggaaga agaacgtctg aacgaactga tgacgcgtta
     2161 cgacgcgctg gaaaaccagt gtgaggaatc tgacctgctg gaagcagaaa tgaagctgat
     2221 gcgctgcatg gcgaaggtca gagcgtggac gccggagatg cgtgccggag gtggagtggt
     2281 ggtgtcctgg cgttatggca acgtgtgtgt ccagcgaggt gtgcagttgc gcagtgagga
     2341 tgacgtggct gacgacgctg accgcacgga acaggtgcag gagaaagcgt cagtgggggg
     2401 aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc acgctggcag tccaggcggc
     2461 gcttatgcag caaccggaca aatctctggc actgctggca tggacgctct gcctgaatgt
     2521 gtttggcagc ggagcgtaca gtaaaccggc acaaatcagc ctggaatgta aacattattc
     2581 gctgaccagc gatgcgccgt cagggaagga aggtgtcgca ttcatggcgc tgatggcaga
     2641 aaaagcccgt cttgctgccc tgttaccgga gggatggtca cgagacatga cgacattcct
     2701 gtcactcagt caggaggtgc tgttgtccct gctcagtttc tgcaccgcat gcagccttaa
     2761 cggtgtccag acccgtgagt gtggtcacac gtcacgcagt ccgcttgact cgctggaaac
     2821 cgctatcgga ttccacatgc gcgactggtg gcagccgaca aaagcaaact tcttcggaca
     2881 cctgaaaaag ccgcagatta tcgcagccct gaatgaggca ggactgtccg gtgctgcacg
     2941 ggatgcagag aagatgaaga aaggtgatgc ggctgaacat gcagagcacc atatgaaaga
     3001 caactgctgg gtgcctggct ggatgtgtgc accacatcca cagacggata ccactgaacg
     3061 caccgataac ctggctgatg ccgcctgata aacaaccaca ccgccccgcc ggagacgggg
     3121 cggcagcaag ggagataccg tgatgaaaac tgaactgacc ctgaatgtat tacagaccat
     3181 gagcgcacag gaatatgaag atatccgggc agccggaagt gatgaacgtc gtgagctgac
     3241 acacgccgtg atgcgggaac tggatgcgcc ggacaactgg atgatgaatg gcgagtatgg
     3301 cagtgagttc gggggctttt tccccgtcca ggttcgtttc acgccagccc acgaacgttt
     3361 ccacctggcg ttatgttcac cgggcgatgt ctctcaggtc tgggtgctgg ttctggtgaa
     3421 tgccggtggt gagccgtttg cggtggttca ggtacagcgg cggtttgcac cggaagccgt
     3481 cagccattca ctggcactgg cggcgtcact ggacgcgcag gggtacagcg tcagtgacat
     3541 catccatatc ctgatggcgg aaggaggtca ggcatgagcg cacgttcaca ggcactggtt
     3601 cctctcagca cagagcaaca ggccgcatgg cgggcggtgg cggagaccga aaaacgccgc
     3661 catcagggta acacgctggc tgaatatccg tatgcggggg cgtttttccg ctgtctgaac
     3721 ggaagccgca ggatatcgct gtctgacctg cgatttttca tgccgtccct gaccgcagaa
     3781 gaactgcacg gcaaccgtct tcagtggctg tatgccatcg atgtgctgat tgaaacacag
     3841 ggcgaggtct gcctccttcc ccttcccggt gatgcagcag agcgcttgtt cccgtcggtg
     3901 cggtttcgtg tcagggaacg cagtcgccat aaatccgcgc tggtcatgca gaagtacagc
     3961 cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt accaggcgct ggtcgctcag
     4021 gctgaaatcg aactggcatt ccattcgccg gaaacagtcg gaagctggca tgcacgctgg
     4081 agtgaccggg tggcagagca tgacctggaa accctgttct ggcagtgggg cgagcgtttt
     4141 ccgtcactgg ccggaatgga acgctggcag tggcaggata tgccattctg gcaggtcatc
     4201 gcagaggctg gtatggcggc aagggtggtc ggtcatgctg ttcgtgagat ggagcggtgg
     4261 atggtgccga acaaactccg ggaggcagcg tgatgcggca acaatcacac ggatttcccg
     4321 tgaacggtct gaatgagcgg attattttca gggaaagtga gtgtggtcag cgtgcaggta
     4381 tatgggctat gatgtgcccg gcgcttgagg ctttctgcct catgacgtga aggtggtttg
     4441 ttgccgtgtt gtgtggcaga aagaagatag ccccgtagta agttaatttt ctgtactgca
     4501 cccattttgt tggacgatga aatggaatag cccctaatat gtcaaagcca aaataccctt
     4561 ttgaaaagcg ccttgaagtc gtgaatcact acttcacaac tgatgatggt tacaggatca
     4621 tctcggcacg ttttggtgtc ccccgaaccc aggtcaggac atgggttgcc ctctatgaaa
     4681 aacatggaga aaaaggttta attcccaaac ctaaaggcgt tagtgctgat ccagagttgc
     4741 gtattaaggt cgtgaaagct gtgatcgagc agcacatgtc ccttaatcag gctgctgctc
     4801 actttatgct tgctggtagt ggttctgtag ccaggtggct gaaggtctat gaagagcgcg
     4861 gagaagctgg tttacgcgcg ctcaagattg gcaccaaaag aaacattgca atatcagttg
     4921 atccagaaaa agcggcatca gcattggagc tgtcaaaaga ccgacgcatt gaggatcttg
     4981 aaaggcaagt tcgatttctt gaaacgcggc ttatgtatct aaaaaagctg aaagccttag
     5041 ctcatcccac gaaaaagtga aagtactcaa cgagctaagg cagttttatc ctcttgatga
     5101 gcttctcagg gctgcggaga taccgcgcag tacgttttat tatcatctaa aggctctcag
     5161 caagcctgac aagtatgcgg acgttaaaaa gcgtattagt gagatttatc acgagaatag
     5221 aggccgatac ggataccgta gggtaacgct gtctcttcat cgagaaggaa aacagattaa
     5281 ccataaagct gttcagcgcc tgatgggaac cctctcactt aaagcagcga ttaaggtcaa
     5341 gcgataccgc tcttacagag gagaggtagg gcaaaccgcc cctaatgttc tccaaagaga
     5401 tttcaaggct acgcggccaa acgagaagtg ggttaccgat gttactgaat ttgcagtcaa
     5461 tgggcgcaag ctgtatttgt ctccagtaat agatctcttc aacaacgaag ttatttctta
     5521 cagcctttcg gaaagaccag tgatgaacat ggttgagaat atgctcgatc aggcattcaa
     5581 aaagcttaat cctcacgagc atcctgttct gcactctgac cagggatggc agtatcgtat
     5641 gagaagatat caaaatatcc ttaaagaaca tggtattaaa caaagcatgt ccagaaaagg
     5701 caattgtctg gataatgctg tggtggagtg tttctttgga accttaaagt cggagtgttt
     5761 ttatcttgat gagttcagta atataagcga actgaaggat gctgttacgg aatatattga
     5821 atactacaac agcagaagaa ttagcctgaa attaaaaggt ctgactccaa ttgaatatcg
     5881 gaatcagacc tatatgcctc gtgtttaact gtccaacttt ttggggtcag tacattcatt
     5941 aaccaccacg aggcatccct atgtctagtc cacatcagga tagcctctta ccgcgctttg
     6001 cgcaaggaga agaaggccat gaaactacca cgaagttccc ttgtctggtg tgtgttgatc
     6061 gtgtgtctca cactgttgat attcacttat ctgacacgaa aatcgctgtg cgagattcgt
     6121 tacagagacg gacacaggga ggtggcggct ttcatggctt acgaatccgg taagtagcaa
     6181 cctggaggcg ggcgcaggcc cgccttttca ggactgatgc tggtctgact actgaagcgc
     6241 ctttataaag gggctgctgg ttcgccggta gcccctttct ccttgctgat gttgtacggg
     6301 catgaacaac ctgacttcag gaaggtcgtt tcccttcagg aacggggatg aacgcgcgcc
     6361 tgcggcgccc gttcttttcc cccgccttct ctggttatgg cctgtcagaa atcacatcac
     6421 cttccctgtg attattctct ttttctt
//
LOCUS       DACADA010000220         6417 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.222, whole genome shotgun sequence.
ACCESSION   DACADA010000220 DACADA010000000
VERSION     DACADA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.222"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            117..1097
                     /locus_tag="ISB17_004408"
     CDS             117..1097
                     /locus_tag="ISB17_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="HAP1982269.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQ
                     NRESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGL
                     GRCLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFV
                     AIDRATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKK
                     KEPSGTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELES
                     TLRNYVKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
     gene            complement(1210..1356)
                     /locus_tag="ISB17_004409"
     CDS             complement(1210..1356)
                     /locus_tag="ISB17_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982270.1"
                     /translation="MKVELGVAAGCNQAATAGIRGLTLSGTKTHVKKSFFNIIEAFIR
                     VIVS"
     gene            1373..2254
                     /locus_tag="ISB17_004410"
     CDS             1373..2254
                     /locus_tag="ISB17_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="HAP1982271.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSI
                     GVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALV
                     PWSPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFR
                     LDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNH
                     RIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAV
                     IAAAARLALEGLGVNGQ"
     gene            complement(2504..3385)
                     /locus_tag="ISB17_004411"
                     /pseudo
     CDS             complement(2504..3385)
                     /locus_tag="ISB17_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
     gene            complement(3489..3785)
                     /locus_tag="ISB17_004412"
     CDS             complement(3489..3785)
                     /locus_tag="ISB17_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor protein KorC"
                     /protein_id="HAP1982272.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDV
                     ADLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
     gene            complement(3831..3986)
                     /locus_tag="ISB17_004413"
     CDS             complement(3831..3986)
                     /locus_tag="ISB17_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982273.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYRE
                     AAATVTH"
     gene            complement(4114..4539)
                     /locus_tag="ISB17_004414"
     CDS             complement(4114..4539)
                     /locus_tag="ISB17_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1982274.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKG
                     LCREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYS
                     HLSFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
     gene            complement(4650..4928)
                     /locus_tag="ISB17_004415"
     CDS             complement(4650..4928)
                     /locus_tag="ISB17_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982275.1"
                     /translation="MIHTANRTFHQLYREWIRERREHMHNVLTWERDRYGARLVGLFY
                     RYCKVANPFPRCTLNTRINYRAHAVNLPDWPARSLELNKMWLSWREKK"
     gene            complement(4912..5307)
                     /locus_tag="ISB17_004416"
     CDS             complement(4912..5307)
                     /locus_tag="ISB17_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982276.1"
                     /translation="MANLPGTGSSSTGWPSSDLDARRKSTRWPTYQAPAAARPGGQVQ
                     IWTPEGNQPGGQLTRHRQQLDQVAHFVRTKSGDAMRNLGGNPPRLRDFFNVERAFSCH
                     SRPFWPIILIKKTGHPPRSIPENSDDPHS"
     gene            complement(5462..6022)
                     /locus_tag="ISB17_004417"
     CDS             complement(5462..6022)
                     /locus_tag="ISB17_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1982277.1"
                     /translation="MRHLFWRLNFLLLSGATNSAQMYHEAAALSSELDTRWSYRSKEL
                     MTLYSKAKSYEAGERVEFGGKSFAPLYTPKNDTLINLFQISQQEQEQLKTIISTDEAQ
                     KRRRERDRLRDEERRRAAGQLEREAYEANSLSRQKPWEAMGMSRAKWYRLGKPSPQQN
                     SETSPSPITNGEASAVALQAARCKAV"
BASE COUNT         1363 a         1890 c         1720 g         1444 t
ORIGIN      
        1 tatttttata ggtgtctgga ctcgtgggat catgtaccca tgcgtagctg gccgctcttc
       61 aaggccagac acgttttgcg tacactgttc caatccgact cttcactggc aactcgatga
      121 cccaggcact gcacagccaa gcccgtacta cccacctgat ccgtgaggaa atcaggaact
      181 cgacgctccc gcaggccgaa ctggccagga tgtacaacgt cacccgccaa accatccgaa
      241 agtggcaaaa ccgcgagtct cctgaagaca agtcgcatgc gccgaacaag atgtacacga
      301 cgctcacgcc cgagcaggag ctcatcgtgg tggagctgcg caagacgttg ctgctgccca
      361 cggacgacct gctggcggtc acgcgcgagt tcatcaatcc agccgtctcg cgtgccggcc
      421 tgggacgttg cctgcgccgc cacggtgtct cggatctacg taacctggtc gagcaggaag
      481 gcactgcgcc cgccacgaaa aagaccttca aggactacga gccgggcttt gtgcacatcg
      541 acatcaagta cctgccgcag atgcccgacg agacggcaag gcgctatctc ttcgttgcca
      601 tcgaccgtgc tacgcgctgg gtcttcatcg agctctatgc cgaccagacc gatggcagca
      661 gtggcgactt cctcaacaaa gtccagcaag cctgtcccgt caagatcgtc aagcttctga
      721 ccgacaacgg cagccagttc accgaccgct tcacggctgg cggcaagaag aaggaaccca
      781 gcggcacaca cgtgttcgac cgcctgtgca agcagctcgg catcgagcac cggctcatcc
      841 cgcctcgtca tccgcagacc aacggcatgg tggagcgctt caacggtcgt atcagcgaca
      901 tcgtcaacca gacccgtttt ggttcagctg ccgaactgga atcgacgctg cgcaattacg
      961 tcaagatcta caaccacagc attccgcaac gcgcgctcca acacaaaaca cccgttcagg
     1021 cgctcaagga atggcatgaa aaacgccctg aattgttcag gaaacgcgtg tataaccagc
     1081 cgggtcttga catataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaatgactt
     1261 cttaacgtga gttttcgttc cactgagcgt cagaccccta atcccagctg tagcggcctg
     1321 attacatccg gccgctacac ctagctccac cttcaaacaa ggaatatcgt tgatgtcact
     1381 gtatcgccgt ctagttctgc tgtcttgtct ctcatggccg ctggctggct tttctgccac
     1441 cgcgctgacc aacctcgtcg cggaaccatt cgctaaactc gaacaggact ttggcggctc
     1501 catcggtgtg tacgcgatgg ataccggctc aggcgcaact gtaagttacc gcgctgagga
     1561 gcgcttccca ctgtgcagct cattcaaggg ctttcttgct gccgctgtgc tggctcgcag
     1621 ccagcagcag gccggcttgc tggacacacc catccgttac ggcaaaaatg cgctggttcc
     1681 gtggtcaccc atctcggaaa aatatctgac aacaggcatg acggtggcgg agctgtccgc
     1741 ggccgccgtg caatacagtg ataacgccgc cgccaatttg ttgctgaagg agttgggcgg
     1801 cccggccggg ctgacggcct tcatgcgctc tatcggcgat accacgttcc gtctggaccg
     1861 ctgggagctg gagctgaact ccgccatccc aggcgatgcg cgcgatacct catcgccgcg
     1921 cgccgtgacg gaaagcttac aaaaactgac actgggctct gcactggctg cgccgcagcg
     1981 gcagcagttt gttgattggc taaagggaaa cacgaccggc aaccaccgca tccgcgcggc
     2041 ggtgccggca gactgggcag tcggagacaa aaccggaacc tgcggagtgt atggcacggc
     2101 aaatgactat gccgtcgtct ggcccactgg gcgcgcacct attgtgttgg ccgtctacac
     2161 ccgggcgcct aacaaggatg acaagcacag cgaggccgtc atcgccgctg cggctagact
     2221 cgcgctcgag ggattgggcg tcaacgggca gtaaggctct gaaaatcatc tattggccca
     2281 ccaccgccgc ccttgcgggc ggcatggatt accaaccact gtcacattta ggctaggagt
     2341 ctgcgcggca gagccgtgtg accggttttc tgtagagcac tgacgatggc ggcggcgctc
     2401 tctgcaattg gcaaggcgtc ggcgccaagg ataccaatct tgcggcgcgc ggcgtgttat
     2461 gacgactggg gtgcatttga gccgccccat ttaaccttcg ccctcacaga tacgccattc
     2521 gcctcagatt tagcgccatg cagacgagct tccactcggc ttgcaccttg tccaggcccc
     2581 tcatgctgaa ctgacgcaat cccatcaccg ccttgatcca accattcgga gcctccacga
     2641 tcgacttgcg ccggcggtaa gctgcatcgc cttgctccgt tttcaatttc gccgcaatcg
     2701 ccgccgtatg cggatgggtc ttggcattga ccttggcatc ttcacgtccc tcgcggccga
     2761 gggcaacgat gacatcgccg tggtgatcgg cgacctttgc cagaacagcc tcactacgga
     2821 atcccgcatc cgccagcgtc tgggccggca tttctccggt gttggcctga actgctgcca
     2881 gcatgcccag cagcgcctga ctgtccgcgg cgcagttggt caactccgcc gccacgatga
     2941 tctggtgctc ggcatcgacc gctgtgtacc cgttgtagct ctgctcggag ccaccaccgg
     3001 cgtgtttcat gatccggctg tccggatcgg tgaagctttc ctgatcacgg tcatccggca
     3061 caccaaactc gcgtttgtac gagccaccgc ccttgtccga gccatccgga tggcgaggcc
     3121 ggcggccatc gtcttcgctg cgcccccggg cctggtccgc ttcacgctgg cgcgcttcca
     3181 ggcgcgcctt tgccgcctgg atcgcctcca ggcgcttctc gcggcgagaa atctcggcag
     3241 gaatgtccag ctccggctcg ttacgctcct ggtcgtcggt agccttggcg cgatcaagca
     3301 gcgccttgat ctcgcaatgc aattcgtcct cggccggctt catgcgctta tagctcatcg
     3361 ccttgtggtc gccatgtttg gcggcttcgt attcaggttc tttagagaga tcctttagat
     3421 cgccgttcct cactactgcc gggaagtctc cccagggagc acgttcggcc ttatgttcgg
     3481 tcatggtttc aatcccggtc agcgtcccat atagccccga aacctgccag gtgggccaag
     3541 atagaccagg caccataagc aatagggcta gggttggttt tgctcaccca acgcctgaag
     3601 gttcgggttc cctttccgct gcctctctca ccaggcaaac caacgagatc agcgacatca
     3661 acgccactga gaggcttact aagccctttt cgttgcctaa tcagctccaa cacctctttg
     3721 atttcatcgg cggttggagc ctgccaatct tcagcgaacg gcctaagcgt ttcaggtcta
     3781 atcatgcgat aaatcctcaa atagccccgg tcaatgccgg ggctttttta tcaatgggta
     3841 acggtcgcgg cggcctcccg atacgccttc agttcaatca agagcaagcc aagctcccgc
     3901 atcttcatgc gcggcatatg aacggggcgg cgggccgtca gagcgtcgcc aatgtgtttt
     3961 gcaagtgata caatatccgt attcatcgcg tttatcctca aattgaccgg tgaaagcccc
     4021 tgtgattggt tgcagccata ccaggggcac catttctttt tctacctatg cacctccttt
     4081 aagagccccg gccatcaccg gggctttctg ttgctagtct attgcggcca agatggcctc
     4141 tttttcgtca tgctcacaag cccaatcccg caagctgtga tacagccttg aaaaccgctc
     4201 ctggtcagcg ccttctgtgt gaaatgacag atggctatag aggtagaggc aagcggtaat
     4261 cccggcagca tcggcgctca tttggccact aaagtagttc atggcctcga tgttcagcat
     4321 ttcatcacga cgcccaggag ccatataaaa gccgccgttc gacagctccc acataagcca
     4381 ataagccccg cgatactcgc ggcagagccc tttcatttgc tggtaaagca tcgactcgaa
     4441 aaccacatag aaacggccta catagcgggg cattgcagcc atacggcgag gggtggcgac
     4501 cagggagcag atcaggggat taagttctgt ttgcatcatc ggagttctcc ggtattgacc
     4561 gtggcgggtg cccggccttc tgagatatat aataggccat tttggcctat tgtgtcaagc
     4621 gggtacaata aaaaataggc caaatcggcc tacttttttt ctctccaact gagccacatt
     4681 ttattcagct ccaggctgcg ggctggccaa tctggcagat tcaccgcatg ggcgcggtag
     4741 ttgatccgag tattcagagt gcaacgggga aaggggttgg ccaccttgca gtaacgataa
     4801 aacagaccga cgaggcgagc accgtaacga tcacgctccc aggtcaaaac gttgtgcata
     4861 tgctcgcggc gttcgcgtat ccattctcga tagagttggt gaaaggtgcg gttagctgtg
     4921 tggatcatcg gagttctccg gtattgaccg tggcgggtgc ccggtcttct taattaagat
     4981 tataggccaa aatggcctac tatggcaaga aaaagcgcgc tctacgttaa aaaaatcacg
     5041 tagtcgcggg ggattgccgc caagatttcg catagcgtcg cctgatttcg tacgtacgaa
     5101 atgggccacc tggtcgagct gctgccgatg cctggtgagt tggccacctg gttgatttcc
     5161 ttctggcgtc cagatctgaa cttggccacc tggtcgagct gctgccggtg cctggtaagt
     5221 tggccacctg gttgatttcc ttctggcgtc cagatctgaa cttggccacc cggtcgagct
     5281 gctgccggtg cctggtaagt tggccacctg gtcgaactgc tgccggatct aaaggtggaa
     5341 tattaagcag gtagtgatag actgagatcc agcatggcta tacgcctgct aacccctcaa
     5401 aagaaagcag cctctagggc tgccatccgt tacgaaatct cactcacttt gaaatagcca
     5461 tctaaacggc tttacacctg gccgcctgaa gggcaaccgc tgaggcttcg ccattagtaa
     5521 taggggaggg acttgtctca ctgttttgtt gaggtgaagg cttgcccagg cgataccatt
     5581 tagcccggct catgcccata gcttcccagg gcttttgcct gctcaatgag ttggcttcat
     5641 aggcttcacg ctcaagctgg ccagcggcgc ggcgacgttc ttcatcacgc agtctatcgc
     5701 gctctctgcg gcgcttctga gcctcgtcag tgctgataat cgtcttcagt tgctcttgtt
     5761 cttgctgtga gatctggaat aggttgatca gcgtgtcgtt tttgggcgtg tatagcggtg
     5821 caaacgactt tccgccgaac tcgacacgtt ccccagcctc ataggatttc gccttgctgt
     5881 agagcgtcat aagctctttg gagcggtaag accaacgagt atccagctcg gaggaaagag
     5941 cggcggcctc atggtacatc tgggcgctgt ttgttgcccc ggagagaagg agaaagttaa
     6001 gacgccagaa aagatgcctc attcgctcgc cttcgttgat tccacctctg agctggccca
     6061 acttgcgtag gtcttccagg gggaaccgca gaattcggaa aaaatcgtac gctaagctaa
     6121 ggaaggtctg aaaaagcccc cgctcgagaa atgcatccca gcaaccatgc gggttacaga
     6181 gcggtgattt cgcgaaacat gggctttttc agagcttcct tagcgttacg ataaagctag
     6241 catggaaacg ataggtgcaa gcaagttaag ggttgcatcg cgcatgtcaa tctaggctat
     6301 accctaactt ggcgtcagac catccggcgc taaatcgtca gaatagagtt gccttccgaa
     6361 ttgattgaca tacgccgtca agggtcatag atttcttcct gacacatttc cctcagg
//
LOCUS       DACADA010000221         6405 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.223, whole genome shotgun sequence.
ACCESSION   DACADA010000221 DACADA010000000
VERSION     DACADA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.223"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            103..993
                     /gene="alsR"
                     /locus_tag="ISB17_004418"
                     /gene_synonym="rpiR"
     CDS             103..993
                     /gene="alsR"
                     /locus_tag="ISB17_004418"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HAP1982278.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1052..1987
                     /gene="alsB"
                     /locus_tag="ISB17_004419"
     CDS             1052..1987
                     /gene="alsB"
                     /locus_tag="ISB17_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HAP1982279.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATETFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2115..3647
                     /gene="alsA"
                     /locus_tag="ISB17_004420"
     CDS             2115..3647
                     /gene="alsA"
                     /locus_tag="ISB17_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HAP1982280.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3626..4606
                     /gene="alsC"
                     /locus_tag="ISB17_004421"
     CDS             3626..4606
                     /gene="alsC"
                     /locus_tag="ISB17_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HAP1982281.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4617..5312
                     /gene="rpe"
                     /locus_tag="ISB17_004422"
     CDS             4617..5312
                     /gene="rpe"
                     /locus_tag="ISB17_004422"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP1982282.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     RERKDLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5296..6225
                     /gene="alsK"
                     /locus_tag="ISB17_004423"
     CDS             5296..6225
                     /gene="alsK"
                     /locus_tag="ISB17_004423"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HAP1982283.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
BASE COUNT         1663 a         1509 c         1632 g         1601 t
ORIGIN      
        1 acaaacttta taaattttat ttttttgtga agttgccagc atcttttctg ttcttgctgt
       61 ggtgatatag tggcgtcttc aattcaagga caagagaacg tgatgagcca gtcagagttt
      121 gattcagcgc ttccgaacgg tatcgggtta gcgccttacc tgcgaatgaa gcaggaagga
      181 atgacagaaa acgaaagccg catcgtggag tggttactca aacccggtaa cctgagttgt
      241 gcacccgcaa ttaaagatgt cgcagaagct ctggcggtat ctgaagcgat gatcgttaag
      301 gtatcaaagc tgctggggtt tagcggcttt cgtaacttac gcagtgcgct ggaagattat
      361 ttttctcagt cagaacaagt attgccgtcc gagttggctt ttgatgaagc gccgcaggat
      421 gtggtgaata aggtatttaa catcacttta cgcaccatta tggaaggtca gtcgatcgtc
      481 aacgttgatg agatccaccg tgccgcccgc tttttctatc aggccagaca gcgggatttg
      541 tacggtgccg gaggatcaaa tgcaatctgt gctgatgtac agcacaagtt tttacgcatt
      601 ggcgtacgct gccaggccta tccggacgct cacatcatga tgatgtctgc ttcgttgtta
      661 caggaaggag atgtggtgct ggtggtgacc cattccgggc gaaccagtga tgtaaaagca
      721 gctgtagaac tggcaaaaaa gaacggggca aagattattt gtataacgca tagctaccat
      781 tcaccgatag cgaagctggc cgattatatt atttgctcac cggccccgga aacaccgtta
      841 ttaggtcgta atgcctcggc aagaatatta caactaactt tgctggacgc gttttttgtc
      901 tctgtcgccc agctcaacat tgaacaagct aatattaata tgcaaaaaac cggcgcaatt
      961 gttgatttct tctcaccagg cgcgctgaaa taagaaaaag gtaattccgc catcgcggag
     1021 ttacctatcc ccttaccgtg agaacatgat tatgaataaa tatctgaaat atttcagcgg
     1081 cacactcgtg ggcttaatgt tgtcaaccag cgcttttgct gccgccgaat atgccgtcgt
     1141 attgaaaaca ctctccaacc cattttgggt agatatgaaa aaaggcattg aagatgaagc
     1201 aaaaacgctg ggcgtcagcg ttgatatttt tgcctctcct tcagaaggcg attttcaatc
     1261 tcaattgcag ttatttgaag atctcagtaa taaaaattac aaaggtatcg ccttcgcacc
     1321 attatcctca gtgaacctgg tcatgcctgt cgcccgcgca tggaaaaaag gcatttatct
     1381 ggtcaatctc gatgaaaaaa tcgacatgga taatctgaaa aaagcaggcg gtaatgtgga
     1441 aggttttgtc accaccgata acgttgccgt cggggcgaaa ggcgcgtcat tcattattga
     1501 caagctgggt gccgaagggg gtgaagttgc aattattgaa ggtaaagccg gtaacgcctc
     1561 cggtgaagcg cgtcgtaatg gtgccaccga aaccttcaaa aaagcaagcc agatcaagct
     1621 tgtcgccagc cagcctgccg actgggaccg cattaaagca ctggatgtcg ccactaacgt
     1681 gttgcaacgt aatccgaata ttaaagcgat ctattgcgcg aatgacacga tggcaatggg
     1741 tgttgctcag gctgtcgcaa acgccggaaa aacgggaaaa gtgctggtcg tcggtacaga
     1801 tggcattccg gaagcccgca aaatggtgga agccggacaa atgaccgcga cggttgccca
     1861 gaacccggcg gatatcggcg ctacgggtct aaagctgatg gttgacgctg aaaaatccgg
     1921 caaggttatc ccgctggata aagcaccgga atttaaactg gtcgattcaa tcctggtcac
     1981 tcaataaaac taaatgatgc cggatagtga tgctaatgaa gtgtcttatc cggccaacaa
     2041 aataacctga acgtagacct gataagtgta gcccatcagg caataatacg atatttcgtg
     2101 cagagaggtt atttatggcc acgccatata tatcgatggc ggggatcggc aagtcctttg
     2161 gtccggttca cgcattaaag tcggttaatt taacggttta tcctggtgaa atacatgcat
     2221 tactaggaga aaatggcgcg ggtaaatcca cgctaatgaa agtgttatcc ggaatacatg
     2281 agccaaccaa aggcaccatt accattaata acattaacta taacaaactg gatcataaat
     2341 tagcggcaca actcggtatc gggattattt atcaggaact cagcgttatt gatgaattaa
     2401 ccgtactgga aaatttatat attggtcgtc atctgacgaa aaaaatctgc ggtgtcaata
     2461 ttatcgactg gcgagaaatg cgtgtccgcg ccgcaatgat gttattacgc gtgggcttga
     2521 aagttgattt agatgagaaa gtggcgaact tatctatcag ccacaagcag atgctggaaa
     2581 ttgccaaaac gctgatgctc gacgccaaag tcatcatcat ggatgaaccc acctcctcac
     2641 tcaccaataa agaggtggac tatctgtttc tgatcatgaa tcagttgcgt aaggagggta
     2701 cggctatcgt ctatatctcg cataaattgg cggaaattcg ccgtatttgc gaccgctata
     2761 cggtgatgaa agacggcagc agcgtttgca gcggcatggt gagcgatgtg tcaaatgacg
     2821 atatcgtccg tctgatggta ggccgcgaac tgcaaaaccg ttttaacgcg atgaaggaga
     2881 atgtcagcaa ccttgcgcac gatacggttt ttgaggtgcg caacgtcacc agccgtgaca
     2941 gaaaaaaggt ccgggatatc tcatttagcg tctgccgggg agaaatatta ggctttgccg
     3001 gactggtcgg ttccggacgt actgaactga tgaactgcct gtttggcgtt gataaacgcg
     3061 ctggcggaga aatccgtctt aatggcaaag atatctctcc acgttctccc ctggatgccg
     3121 tgaaaaaagg gatggcttac atcactgaaa gccgccggga taacggcttt ttccccaact
     3181 tttctatcgc tcagaacatg gctatcagcc gcagtctgaa agacggcggc tataaaggcg
     3241 cgatggggtt gtttcatgaa gttgacgagc aacgtaccgc tgaaaatcaa cgcgaactgc
     3301 tggcgctgaa atgtcattcg gtaaaccaga acatcactga actctccggc ggtaaccagc
     3361 agaaagtcct gatctccaaa tggctgtgct gtcgcccgga agtgatcatt ttcgatgaac
     3421 ctacccgtgg catcgacgtt ggcgcgaaag ccgaaattta caaagtgatg cgccaactgg
     3481 cggacgacgg aaaagtcatc ctgatggtgt catctgaact acctgaaatt atcaccgtct
     3541 gcgaccgcat cgccgtgttc tgcgaaggac gactgacgca aatcctgacg aatcgcgatg
     3601 acatgagcga agaggagatt atggcatggg ctttaccaca agagtaaaaa gcgaagcgag
     3661 cgagaagaaa ccgttcaact ttgcgctgtt ctgggataaa tacggtactt tttttatcct
     3721 ggcgatcatc gtcgccattt ttggttcgct gtcgtcagaa tattttctga ccaccaataa
     3781 tattacccag atttttgtgc aaagctccgt gacggtactg atcggcatgg gggaattctt
     3841 cgctatcctg gtcgccggta tcgacctttc ggttggcgcg attctggcgc tttccggtat
     3901 ggtgaccgct aaactgatgt tggcaggtat tgatccgttt ctcgcggcgc taattggcgg
     3961 tgtactggtt ggcggcgcac tgggggcgat caacggctgc ctggtcaact ggacgggact
     4021 acacccgttc attatcaccc ttggcaccaa cgctattttc cgtgggatca cgctggtgat
     4081 ctccgatgcc aactcggtat acggcttctc atttgacttc gtgaacttct ttgccgccag
     4141 cgtaattggg atacctgtcc ccgttatctt ctcgctaatt gtcgcgctca tcctttggtt
     4201 tctgacaacg cgtatgcggc tcgggcgcaa catctacgca ctgggcggca acaaaaactc
     4261 ggcgttctat tccgggattg acgtgaaatt ccacatcctg gtggtgttta tcatctccgg
     4321 tgtttgtgca ggtctggcag gcgtcgtctc aactgcacga ctcggtgccg cagaaccgct
     4381 tgccggtatg ggttttgaaa cctatgccat tgccagcgcc atcattggcg gcaccagttt
     4441 cttcggcggc aaggggcgca ttttctctgt ggtgattggc gggttgatca tcggcaccat
     4501 caacaacggt ctgaatattt tgcaggtaca aacctattac caactggtgg tgatgggcgg
     4561 attaattatc gcggctgtcg cccttgaccg tcttatcagt aagtaaggaa ttgaacatga
     4621 aaatctcccc ctcgttaatg tgtatggatc tgctgaaatt taaagaacag atcgaattta
     4681 tcgacagcca tgccgattac tttcacatcg atatcatgga cggccacttt gtgccgaatc
     4741 tgacgctctc accgttcttc gtaagccagg taaaaaaact ggcgagtaaa ccgctcgact
     4801 gccatctgat ggtaacgcgt ccgcaggatt acattgctca actggcacgt gcgggagctg
     4861 atttcatcac tttgcatccg gaaactatca acggtcaggc attccgcctg attgatgaaa
     4921 tccgccgtca tggcatgaaa gtggggctga ttcttaatcc ggaaacgccg gttgaggcca
     4981 tgaaatacta tatccataag gctgataaaa ttacggtcat gactgtcgat cccggttttg
     5041 ccggacaacc cttcattcct gaaatgctgg ataaagttgc cgaactgaag gcatggcgtg
     5101 aacgaaaaga tctggagtac gaaattgagg tggacggttc ctgcaaccag gcaacttacg
     5161 aaaaactaat ggcggcaggg gcggatgtct ttatcgtcgg cacttccggc ctgtttaatc
     5221 atgcggaaaa tatcgacgaa gcatggagaa ttatgaccgc gcagattctg gctgcaaaaa
     5281 gcgaggtaca gcctcatgca aaaacagcat aacgtcgtag cgggcgtgga tatgggggca
     5341 acgcatatcc gcttttgtct gcggacagca gaaggtgaaa cgctacactg cgaaaaaaag
     5401 cggaccgcag aagttattgc tcccgacctg gtgtcgggta tcggcgaaat gattgacgag
     5461 caactcaggc gctttaacgc tcgctgtcat ggtctggtga tgggatttcc ggcgctggtc
     5521 agtaaagata aacgcaccat tatttctacg cctaacctgc cgttaacagc ggcagattta
     5581 tatgatctcg ccgataagct cgaaaatacg ctgaattgtc cggttgagtt ttcccgcgac
     5641 gttaacctgc aactttcctg ggacgtagta gaaaaccgcc ttacgcaaca actggttctg
     5701 gcggcctatc tcggtacggg gatggggttc gcagtgtgga tgaacggtgc gccgtggacg
     5761 ggtgcacacg gtgtggcagg cgaactgggc catatccccc tgggagatat gacccaacac
     5821 tgcgcgtgtg gcaattctgg gtgcctggaa accaattgct ctggaatggc gctaagacgc
     5881 tggtacgaac aacagccccg aaattaccca ttgagcgatc ttttcgtcca tgcggaaaac
     5941 gcccctttcg tccagagtct gcttgaaaac gcggcacgag ccattgccac cagcattaat
     6001 ctgttcgatc ccgatgcggt gattctgggc ggtggcgtga tggatatgcc cgccttccca
     6061 cgcgagactc tcattgccat gacccaaaag tacctgcgcc gtccactgcc gtatcaggtc
     6121 gtgcgcttta ttgccgcctc atcttctgac tttaatggcg ctcagggtgc agcaatattg
     6181 gcgcatcaac gttttttgcc acagtcctgt gctaaagtcc catgagtaaa gcgttggcac
     6241 agtagcgcaa tgtcccgctg tgccaacgac aggtacaata tatataataa ttacataaaa
     6301 cagcccatat tattcccagc aatgatagtt ctactgtaat tttccctctt cagcacaaat
     6361 gcctaataaa atggctcgtt ccgctcacac ttccacttat taaag
//
LOCUS       DACADA010000222         6256 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.224, whole genome shotgun sequence.
ACCESSION   DACADA010000222 DACADA010000000
VERSION     DACADA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.224"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(240..1433)
                     /locus_tag="ISB17_004424"
     CDS             complement(240..1433)
                     /locus_tag="ISB17_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP1982284.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
     gene            complement(1631..4264)
                     /gene="mngB"
                     /locus_tag="ISB17_004425"
     CDS             complement(1631..4264)
                     /gene="mngB"
                     /locus_tag="ISB17_004425"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAP1982285.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQLGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIGTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRTFSYRIV"
     gene            complement(4282..6198)
                     /gene="mngA"
                     /locus_tag="ISB17_004426"
     CDS             complement(4282..6198)
                     /gene="mngA"
                     /locus_tag="ISB17_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAP1982286.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
BASE COUNT         1616 a         1654 c         1351 g         1635 t
ORIGIN      
        1 aacatgactc cactttcatt tttatgaaat ggcatcgtga agaggcgcca gccaattata
       61 aagatcttgc tcctcaaaaa atgaaacaac tcagcctgag ataatatttt tgaccgaagt
      121 taacattaaa aatggttaat agtttgcgcc tctcacacag gtccaggcaa ctgcgtaaca
      181 ggcaaatcaa attttcgtaa acagtgggca tcatcagatg cccacaaaat acagctgatt
      241 tacattttcg tctttacacg tgcaatagct tcttcataag aaggcttatt tttttccgta
      301 tacgccagtt gataatgctg caatgcttca gcattctggc ccgaggtttc ataaattttg
      361 gcaatgttat aataggagtt ggcgcgcgtt gtcgccgcat ttaccccact cgctaacgag
      421 atcgctttac gatttgccca aattgcttcc gctatgttgc catttttctg atacgccaga
      481 cccaggttac taaatgcctg tccgtaattg ccgtctaatt ctgttgcctg ttggaagagt
      541 tgaatggctt gctcaagttg accggtttta taaacagctt caccttcgcg attatatttt
      601 cttgccagtt gagtatctcc actgttttgc atcgcaggcg aagccggagc tgaagagtcc
      661 gtcgcaggca ttatcacagt ggctaaatcc ctgacgtcta atgtggtgcc gttaaaattg
      721 gcgctggtaa cagagttgat atcttcaaac tcgccgtttg gattgagttt aaacacggtc
      781 cagatgttcc ctcgttttcc tgcagggaca ctataactac gtaccaggga gctccctaca
      841 taaacaaaca cttttgcctt actggcagag agataattac tgttgggcag ccctttattg
      901 ctatagtcct gcacggcata gatgtaactt tcaccaaaat gctttttgct gatagtgaca
      961 gtttctggtc catagctatc cgtatcatca acatccagat tggcatcagt tccctcttta
     1021 ctatcaaaat agatatgtcc gccagggaag atcaaatgcg aatcaagatc aaaaggcttt
     1081 tcgccccagc tcaaaactac acgcatgcca tccagattcg tcatcaccgg actgatagca
     1141 taagtcatgc cagcgcacgg acatttgacc accagattgg aatacccctc ttttttgacg
     1201 attaatagag catctttatt atcagcaaat gaagtcccca aactaatgct gcccgaagta
     1261 tttgtgatac cagaaacaga ttgtgcacca ttgcgttgca gcgtcaccga tgccccttca
     1321 atgcgtttat ctttcactgt cgcacttaaa atatcaatgc ttacatcggc ggcataagca
     1381 cctgcattca tgcccaacag cgcgagagca actggcaaaa caattttctt catgtataaa
     1441 tccttttttt aatgaatgta aggtggaaat ttcccagtta ttattatcgt gttaaaaggt
     1501 gaaaaaaaag aaataagtac acttttcaga ttaacaccca ctcaccttga aacaaaaagg
     1561 ccatccggag atgacctgaa ctttctgaga aacgctgttt tttcatgtgg tttgctttat
     1621 tacacaccat tcagactatt cggtagctaa atgtccgcga ctgtcccggt aaaaagagag
     1681 ctaattcttt taaaacctca tttttttctg tattaaagcg ttcattcatc atggtttctg
     1741 tacacgtcat caccttcctg ctaaacgcga cggtcgcatc acagcttgtc aattcagccg
     1801 gattaaacag ccgcaatatt aattcctgtc tgtcttctgc tttttttagc gcgctgatca
     1861 gacatcccac tggtggcatt ttcagcaaac tatagctttc cggcacattg aatttggctt
     1921 tattaagctt catcgcatcc cacgggattt tgttataaca ctgaactggc gtcagccatg
     1981 cgcgtgcctg ctgggctaca ccggcagtta cgggagtacc gatataacta aataaactca
     2041 gacgacagga aaatgatccg cgtaattgtg aatcggacac aggcatttta attcctgaag
     2101 gacgccctgg ccttaaaagt aaatcttctt ttcccagtaa tcccacacca cgcaacaacg
     2161 taatagcaaa ggttttattc tcttctccaa tgacttcaaa ttcacgtaat ccttcgctaa
     2221 aaacggctat gccgttacgc ccttcctgta agacagcata gttgagcata ttccataccg
     2281 gaaccggcgc ttctttccag ccttcttgct gccagacatt cattgcgcaa tccttcactg
     2341 gccgcgtcaa tgaaccaaac tgcgtatccg caagaacaac atctgtgtta aaaggcgtgg
     2401 gaatcaatac acgaatacgg tgatcatcgg cctggttatc aagattaata tccgcatcaa
     2461 tgcgtctgct gttatggcta agagtgatca ccgtctccac ccctaccctg ccagaacatt
     2521 gctttgcgcg acgctctgac agattacgcg gcaccgctat ttcatagcga attatggccc
     2581 tactctgcca ggcttcatga ataatatcgt attgcggttt cgcattcgct gaggtaatgg
     2641 cccactcttc ttttgctggc gaataatcat attcatcccc atcatcggaa ccttcttcaa
     2701 tttgaaacac ccggtcatag cgcacgccac tatctttatc aaccagacga agtgaaccat
     2761 cctcattgag tgcaatttgc cagaaagcat tttccagtat tccctctgtt ttactttttg
     2821 gcgtgaccac attgccaagc tgattcgctt ctatatagag cgtgcgatac cccatagaag
     2881 ggacaatttg gctgatctga atatcaaact ccataaaggg atcataattg ccgtaatgaa
     2941 ctatttgccg atcaataagg ccgggatcga tctcacgggc atgtcgaata aaatatggca
     3001 caggctgacc gcgaccgtct cgcaaattaa actggctacc gcgcagtcga acagttgtgt
     3061 tgataacttc ttcacgcggc cagggcatca ggttaaacag gacgagtttg tcggcgtcgc
     3121 tctgcggcat gttgtctgct attttgcgca tatagaaccg aattagatta tccgccatgt
     3181 cttctgccag ttcgaagcgg gcgacaatct cgcgatgaac tttatcactg cagcagcagc
     3241 cgatgctgtc atgggcgtgg ttttttaaaa tttctttcca catcttctcc agcaagccgt
     3301 ggtgataatc aaaacctaaa gtccaggcca gcgttgccaa cggttccagc agattaacaa
     3361 tcttattttc aatgcgtgcg tgggcaattt tgatatccat gcgcgtggaa ccgatggtgc
     3421 gatgcacgcg catatatttg ccatcgatga attcaccttt caggatggcc agactttcac
     3481 gctgcgcttc aatctgctcg aatacctctt caaagcggct catcacaaac tttcgttgag
     3541 gataaatttc gcgcagtttc tccatcactt cgaagatatt ttgctgcaag ggcatctggt
     3601 catgaccatt cggcaataaa atttctttgg ttactgacgc tttttccagc acgtcaaaat
     3661 aactgtcgag gcgtttacgt aatccatttt cgtccgcagg taagtactta ccgatggcgt
     3721 agcccagcgg cagcacctgc gccgtcactt cgctaccgtc actgctttgc cacaaaaact
     3781 cggttttatc agtaccgtgg cgctcagaac atccgcgcca gaacatggtg cgggtaatgc
     3841 caaatccatt gtatatatgt ggcagttgcc cggacatgcc aaaggaatcc ggtaaataac
     3901 ctattttcat cggctcgcca aacgcgaggc agtcacgcat tccgtacatc agattacgga
     3961 ctatggattc agcagaaaca atcgtggtat cggtctgggt ataccaggga ccgataatta
     4021 atttcccgcc ctcgaccaac ttcttcacac gatctttgtt ctccggtttg acagcgaaat
     4081 aatcttcgag gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg ttgtcctgtt
     4141 ccagtctgca caggatctct tccatattat tgaccagcag aatgcgagac tcttctgtgg
     4201 tgaaatacca ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga gataccgctt
     4261 tcatcgtcgt ttcctttttg tttatggcat tacgccatca gtcagatagt ttccatgctt
     4321 taccgcatga tgccgccaga tcaggagaat tgcagtcgag attgcagccc ccaccaacgc
     4381 cgcaccaaac caaccaattg ccgccataac accacccgcg ccattatcat gaagtaaaaa
     4441 gagcgagaaa atgccggcac cgggtgtcga aaggccgata ttcatcgcac cgacgatagc
     4501 gcccgttacc atagatccca gcacaaacga accaataacc cgcagcggat cttcaatcgc
     4561 catcgggatc gccccttcgg tgatacctgc caatcctaac agccaggtgg atttcccggt
     4621 ttcaatttca aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg ttacggtaaa
     4681 tgccgaaacc attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc cgttcgccat
     4741 tgcccccaga cagaatgcat aagcggcttt gttcactggc ccaccaaggt caaaggaaca
     4801 cataaaaccg agaatggcgc ccaacaacag tgcgttattt cctgacagac cgttcagcca
     4861 ggcggtaagc gagttattga tccatgcgac aggttcccct actacaaaca gcatcagact
     4921 acccgccccc aacgtaccga gcaccgggta gagatagaaa gtcaggaatc cgttgaattt
     4981 actgctaaga cgtagatgat ttttcaccca gcgcatcaga taaccggcta tcaatccgcc
     5041 aacgaccgcg ccgagaaacc cggaaccaat catgttggcg gcaagccctg ccgcaaagcc
     5101 tggcgctaac gccggtttat cagccagtga ataggcggta taggctgcga gcaccggtac
     5161 catcaaaatc ccgagcatcc cgccgcccag cttgcggtac atccacaacc aggagttttc
     5221 ttcattaaac aaatcttgta gcccgaagat ttgcgacagt aataccgcga ccgccagcac
     5281 cgtgccaccc gcgacaatca gcggcacggc gaaagagatc ccgctcaaca gtgcctgctt
     5341 cagctccgtt ttgacacttt tcaccggttg cgtatcttgc tgtacggtac gcgtctcatc
     5401 gctacgcttg agggtaagcg cttgttgaat caacgcttct gcatggcgaa tcggctcggc
     5461 aacaggcaca gaaagcgcgg gaatgccgtt aaaacgctcg ctctccttga tagcgacttc
     5521 cgccgcaaaa atacaggcgg ttgcactatt gagttgctcc gccgttaaac gctcttcaat
     5581 gccgttagca ccttgctttt caacgtagat attcacgccg agtttgcgtc cggctttttc
     5641 cagatattcc gcagccatgt aggtgtgagc aatacccgcc gggcaggccg ttacgcagac
     5701 gatagttggc gcataagaaa aagaggcagt atgttgcgcg tcccccttgt catccagcgc
     5761 cgagagcaat tcatcgggcg tcgttgccga ctgaatacgc gccctgagct catcatccgc
     5821 caggcgcgtg gtcagcgcgg taagcagttt catatgcgta gtacctgctt cattgggagg
     5881 aatggccagc aataccacta aatcaaccgc ttccgggcca tccacgcctt cccactgaag
     5941 cggctcgctg agtgtggcga ccgcaaacgc cgcttctttt accgcagcag ttttgccatg
     6001 cggcacggcc agcccttcac ctaaggcggt cgggccgagg ctttcacggc aatacacttc
     6061 ttccagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt gagtcaacgc
     6121 gtggatggcc tcttcacggc tggtaaagcg cacattcaga cacagcgcat cgcggtgggt
     6181 cagagtcgtc aggttcatca tggttctcac ttgatcattt ttatagtcgc gcaagtgtgc
     6241 ctgataaaac aataca
//
LOCUS       DACADA010000223         6239 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.225, whole genome shotgun sequence.
ACCESSION   DACADA010000223 DACADA010000000
VERSION     DACADA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.225"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..212)
                     /locus_tag="ISB17_004427"
     CDS             complement(<1..212)
                     /locus_tag="ISB17_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982287.1"
                     /translation="MLNTRVHQSEVSMATDTPRIPEQGVATLPDEAWERARRRAEIIS
                     PLAQSETVGHEAADMAAQALGLSRRQV"
     gene            complement(251..919)
                     /locus_tag="ISB17_004428"
     CDS             complement(251..919)
                     /locus_tag="ISB17_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1982288.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            complement(955..1191)
                     /gene="merE"
                     /locus_tag="ISB17_004429"
     CDS             complement(955..1191)
                     /gene="merE"
                     /locus_tag="ISB17_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HAP1982289.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            complement(1188..1550)
                     /gene="merD"
                     /locus_tag="ISB17_004430"
     CDS             complement(1188..1550)
                     /gene="merD"
                     /locus_tag="ISB17_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HAP1982290.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            complement(1568..3262)
                     /locus_tag="ISB17_004431"
     CDS             complement(1568..3262)
                     /locus_tag="ISB17_004431"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HAP1982291.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            complement(3314..3736)
                     /gene="merC"
                     /locus_tag="ISB17_004432"
     CDS             complement(3314..3736)
                     /gene="merC"
                     /locus_tag="ISB17_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HAP1982292.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            complement(3772..4047)
                     /gene="merP"
                     /locus_tag="ISB17_004433"
     CDS             complement(3772..4047)
                     /gene="merP"
                     /locus_tag="ISB17_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HAP1982293.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            complement(4061..4411)
                     /locus_tag="ISB17_004434"
     CDS             complement(4061..4411)
                     /locus_tag="ISB17_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HAP1982294.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            4483..4917
                     /gene="merR"
                     /locus_tag="ISB17_004435"
     CDS             4483..4917
                     /gene="merR"
                     /locus_tag="ISB17_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HAP1982295.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            4996..6000
                     /locus_tag="ISB17_004436"
     CDS             4996..6000
                     /locus_tag="ISB17_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAP1982296.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
BASE COUNT         1259 a         1875 c         1912 g         1193 t
ORIGIN      
        1 acctggcgcc gagacaagcc cagcgcctga gccgccatat cggccgcttc gtgcccgacc
       61 gtctccgact gcgccaacgg actgatgatc tccgcacgac ggcgcgcacg ctcccaagcc
      121 tcatcaggca gagtggccac gccttgttct ggaatccgtg gggtgtccgt cgccatgctc
      181 acctcgcttt ggtgcacacg agtattgagc atagtcgaga ttggtgcaga tcacttctga
      241 tattgaactg tcaggagctg gctgcacaac agccattacg cccaatcaac tggtgcagtc
      301 gtcttctgaa aatgacatcc atgcccagcc cgtgcgcgag ctggatcacc gcccgcacga
      361 tagtttggtc acgggcatca tccgggagcc tggcgacaaa ggattggtcg attttcaatg
      421 tggtgatggg gcagcatttc agatgttgca ggcaggaata gccggtgccg aagtcgtcgg
      481 cggcgaagcg cacgccgatg gcgcgcaagg cgtcgaaact ggcgaacagg gctggattgc
      541 cgaatgcgac cgattcggtc agttcgatct ccagaagctc ggcgggcagg gccatatcgg
      601 ccagcacccg ctttacctcg tcgtcgaacg ttggcccaac ctggctggcg gacacattga
      661 tggcaagacg gaacggttgc catgccggtc cttgccactt gtgcatctgg cgacaggcct
      721 cgcccagcac ccacgcgcct atttccggca tcaggccgaa cgactcggcc agcggcagga
      781 actggccggg cggcaacagg ccaagcctcg gatgccgcca gcgcatcaac gcttccgcgc
      841 cagcgatccg gtgatcgcgc agatcgacca gcggctggta atgcaggtca agctgtccgc
      901 gcgccgccgc ctgcgccaac tcggccgccg tccatccggc gggctgcgaa ctcgtcatga
      961 tccgccccgg aaggcgcgca gcagccgcgt tacggccaga acgaacaagc cggtcagcgc
     1021 gagcgcggca acaccccaat gctcgccaag gaaggcaccg gcggtcgtcc cggccagcac
     1081 ggcggcgaga atcggcagat ggcaggggca ggtcaacacg gccagcgcac cccacaggta
     1141 gccggaaacg ggttggcgcg tctcgggcgg cagtttgtca ggggcgttca cggcaatgcc
     1201 tcctcgtgcg cccgctcggc tggcatggag gccagttgcg cgtccagatg ggccaacgcc
     1261 gcgcgccgcc gctcgaccaa ctggcgcagc acggcaagct gcgctgcggc ttgtgcgccg
     1321 tccgctgcgt cgagcgcacg gcacagccgc gccagggcat ccaggccgat acccgcctcg
     1381 aaggccgcgc gcacgaagca cagccgttgc aaggccgcat cgtcgaacac gccgtagccg
     1441 cccgtggtgc aggccaccgg ccgtaacaag ccgcgcacca ggtagtcgcg cacgatatgt
     1501 acgctcaccc cagcgttatg ggccagttgc gataccgtgt aggcgctcat cgcacacctc
     1561 cttgtcctca cccggcgcag caggaaagct gcttcacatc cttgttgaag gtctgcgccg
     1621 cgagcttcaa cccttcgacc atcgtcaggt aggggaacaa ctggtcggcc agttcctgca
     1681 ccgtcatccg gttgcgaatc gccagtgcgg ccgtctggat cagttcgccc gcttccgggg
     1741 ccactgcctg cacgccgatc agtcgtccgc tgccttcttc aaccaccagt ttgatgaagc
     1801 cgcgcgtgtc gaagttggcg agcgcgcgcg gcacgttgtc cagcgttagc gtgcgactat
     1861 cagttttgat gccgtcatgg tgcgcttccg cctcgctgta gcctacggtc gccacttgcg
     1921 ggtcggtgaa caccacggcc ggcatcgcgg tcaggttcag ggccgcgtca ccgccggtca
     1981 tgttgatcgc ggcgcgagtg ccggccgctg ccgccacata gacgaactgc ggctggtcgg
     2041 tgcagtcgcc tgcggcgtag atgtgttcca cgcttgtacg catgccgggg tcgatgacga
     2101 tagcgccttg cggggtgagc gtgacgcccg tcgcatccag tgccagcttg cgtgtgttgg
     2161 gcgcgcggcc ggtggcgacc agcagcttgt cggcgcgcag ttcgccgtgc gccgtggtga
     2221 gcacgaattc gccgtcccct tcaccattga tatacgcgac ctggctggcc tgggtgtgtt
     2281 ccctcacctc gatgccctcc atgcggaatg cggccgtgac ggcttcgcct atagctgggt
     2341 cttcgcggaa gaacagcgtg ctgcgagcca ggatcgtcac cttcgctccg agtcgggcga
     2401 acgcctgcgc cagctccagc gccaccactg atgagccaat cacggccagg cgcttaggaa
     2461 tcgtctcgct gaccagcgct tcagtggaag tccagtacgg agtgtctttc aggccgggaa
     2521 tcggcggcac ggccgggctc gcgccggtgg cgatcaggca gcggtcgaat gccaccacgc
     2581 gctcgccgcc gtcgttgagt tgcacgatca ggttgcgatt gtccttaaag cgggcggagc
     2641 cgtgcagcac agtgatcgcc ggattgccct ccaagatgcc ttcgtacttg gcgtggcgca
     2701 gttcatcgac gcgggcctgc tgctgggcca gcagcgccgt gcgctggatg gtcggcgtgg
     2761 tagcggcgat gccgccatcg aacgggcttt cccggcgcag atgggcgata tgggcggcgc
     2821 ggatcatgat cttggacggc acacaaccga cattgacgca ggtgccgccg atggtgccgc
     2881 gctcgatcag cgtgacacgt gcgccttgct cgacggcctt cagcgccgct gccatcgcgg
     2941 ccccgccgct gccgatgacg gcgatatgca atgcgccgct gctacccgtc ttgtcgtttc
     3001 tgcccagcag atcgcgcatc ttgtcgagca atccgcccgg cgtcgaaact gagggggcat
     3061 cggccagcgt ggcccgataa ccgagtccag ctacagcggc cgtcagcgcg tcgggtgacg
     3121 tgccgacctc aatggcgagc ttggcgctgc ccttggcgta ggagacatcc gctgattgca
     3181 cgccgggcac tttctccagg gcgtccttga catgcactgc gcacgagtcg caagtcatgc
     3241 cggtgatttt gagagtgctc ataccatcgt tccttattcg tgtgggccgc cgtgtcgcac
     3301 ggtcagccgt ctttcacaag cgcttggcgg ggagttcgca gccgtccggt ccgcaacggc
     3361 gatgcgccgg cgacacgaag tcccagatcg acaccccaat catcaaggcc aggccgacgt
     3421 acatcaggtt cgccgtccac cagttgccga gcagccagac cgtggccgca aacacgatgg
     3481 ccgggccgat catgccgagc agactgcgca gccattgccg atgactgaac caacccagcg
     3541 cgttcgccag gaaggccagc gcggcaaaca gcggcagcag gcggctgatg aacagtccct
     3601 cgtactggct caagaagccc agcccgatgg ccgcgccgaa gctggcgagg gctggaaagc
     3661 aggcggcgca gcccatcgcg gaaacgacgc tgccgagcgc gccggtttta tcggcaatgc
     3721 gtgtcatcag tcccatgaag cggctctcgc tgttgtcgtt ggcttgctgg ctcactgctt
     3781 gacgctggac ggatagccgg cgtctgcggt ggccttggtc agcttctgta cgctggcctt
     3841 ggtgtcgtca aaagtgacga cggcctcgcg cttctcgaag cccacatcga ccttgctcac
     3901 gccttcgacc ttggagagcg ctttcttgac tgtgatcggg caggcggcgc aagtcatgcc
     3961 gggaaccgct agcgtgacgg tctgggtagc ggcccacacc ggggcaacag cggcggcgag
     4021 ggcaagggag gcaaacagtt tcttcatgat gaactcctgg ttaatagaaa aatggaacga
     4081 catagggaaa tccaagcgcg accaggacca gcacggccac gatccagaaa atcagcttgt
     4141 aggtggcgcg cacctgcgga atcgcgcaga cctcacctgg cttgcatgcc tgcacgggcc
     4201 ggtaaatccg cttccaggcg aagaacagcg ccactagcgc cgcgccgatg aacaacggtc
     4261 gatagggttc cagcaccgtc aggttgccga tccaagcacc ggagaagccc agggcgacca
     4321 gtactagcgg ccccaggcag caggtcgatg caagaatggc ggccagcccg ccggcgaaga
     4381 gcgcaccgcg cccgttttgt ggttcagaca tacgttggcc cttttgaatt tggattggat
     4441 agcgtaacct tacttccgta ctcatgtacg gagtcaagcg atatggaaaa taatttggaa
     4501 aacctgacca ttggcgtttt tgccaaggcg gccggggtca acgtggagac aatccgcttc
     4561 tatcagcgca agggcctgtt gcgggaaccg gacaagcctt acggcagcat ccgccgctat
     4621 ggggaggcgg acgtggttcg ggtgaaattc gtgaaatcgg cacagcggct ggggttcagt
     4681 ctggacgaga ttgccgagct gttgcggctc gacgatggca cccactgcga ggaggccagc
     4741 agcctggccg aacacaagct caaggacgtg cgcgagaaga tggccgactt ggcgcgcatg
     4801 gaaaccgtgc tgtctgaact cgtgtgcgcc tgccatgcac gaaaggggaa tgtttcctgc
     4861 ccgttgatcg cgtcactaca gggcgaagca ggcctggcaa ggtcagctat gccttagcgt
     4921 gctttattta atgagatggt cactccctcc ttcccagtac tatgctgagg acaggctttc
     4981 attcggagaa ccatcatgga aaacattgcg cttattggta tcgatctggg taagaactct
     5041 ttccatattc attgtcagga tcatcgtggg aaggccgttt accgtaaaaa attcacccga
     5101 ccaaagctaa tcgaatttct ggcgacatgc ccggcaacaa ccatcgcgat ggaagcctgt
     5161 ggcggttctc actttatggc acgcaagctg gaagagttag ggcattttcc aaagctgata
     5221 tcaccgcaat ttgtccgccc attcgttaaa agcaacaaaa atgacttcgt tgatgctgaa
     5281 gctatctgtg aagcagcatc acgtccatct atgcgtttcg tgcagcccag aaccgaatct
     5341 cagcaggcaa tgcgagctct gcatcgtgtc cgtgaatccc tggttcagga taaggtgaaa
     5401 acaactaatc agatgcatgc ttttctgctg gaatttggta tcagcgttcc gcgaggtgct
     5461 gccgttatta gtcgactgag tacccttctt gaggacagta gtttgcctct ttatctcagc
     5521 cagttactgc tgaaattaca acagcattat cactatcttg ttgagcagat taaagatctg
     5581 gaatctcagt tgaaacgaaa gttggacgaa gatgaggttg gacagcgctt gctgagtatt
     5641 ccctgcgttg gaacgctgac tgccagtact atttcaactg agattggcga cgggaagcag
     5701 tacgccagca gccgtgactt tgcggcggca acagggctgg taccccgaca gtacagcacg
     5761 ggaggtcgga cgacattgtt agggattagc aagcggggca acaaaaagat ccgaactttg
     5821 ttggttcagt gtgccagggt attcatacaa aaactggaac accagtctgg caagttggcc
     5881 gactgggtca gggagttgtt gtgtcggaaa agcaactttg tcgtcacctg tgctctggca
     5941 aacaagctgg ccagaatagc ctgggcactg acggcgcgac agcaaactta cgaagcataa
     6001 aggcagaaat acaccagttt aaacaatcat tcatctggtt ttgcgaatac tgatattgat
     6061 gatactaacg gcccaccggc ctgttgagga acctgtaaaa cggaaaggct cattgaagcc
     6121 gtatattttc tggaggttca tcaggcgcgg aactcatcga ggcgcgggaa taaaatccca
     6181 ttcagacgcc ggatagattc aagcaagcca acttgtcgtc aaaatcggtg ttgcaaaaa
//
LOCUS       DACADA010000224         6233 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.226, whole genome shotgun sequence.
ACCESSION   DACADA010000224 DACADA010000000
VERSION     DACADA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.226"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..62)
                     /locus_tag="ISB17_004437"
     CDS             complement(<1..62)
                     /locus_tag="ISB17_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982297.1"
                     /translation="MNVGLGEGSALPVGVPVPWPT"
     gene            122..694
                     /locus_tag="ISB17_004438"
     CDS             122..694
                     /locus_tag="ISB17_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1982298.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMERELIVERTLAGLAAARAQGRVGGRRPKLTKEQHEQIARLLQKGYD
                     RKRLAIIYDIGLSTIYRYHPVGTVITQPEM"
     gene            complement(950..2233)
                     /gene="aroA"
                     /locus_tag="ISB17_004439"
     CDS             complement(950..2233)
                     /gene="aroA"
                     /locus_tag="ISB17_004439"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP1982299.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(2304..3392)
                     /gene="serC"
                     /locus_tag="ISB17_004440"
     CDS             complement(2304..3392)
                     /gene="serC"
                     /locus_tag="ISB17_004440"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAP1982300.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(3591..4283)
                     /locus_tag="ISB17_004441"
     CDS             complement(3591..4283)
                     /locus_tag="ISB17_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP1982301.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            4413..6173
                     /locus_tag="ISB17_004442"
     CDS             4413..6173
                     /locus_tag="ISB17_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAP1982302.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1644 a         1564 c         1472 g         1553 t
ORIGIN      
        1 gtgggccacg gaacaggtac accaacgggc agcgccgagc cttcccccaa accaacgttt
       61 atctctcaaa tcctccccac attatctcag tactttaacc actcaaaagg gagtattttt
      121 aatgctgatt ggctacatac gtgtgtcaac aaatgaccaa aacacagatt tgcaacgcaa
      181 tgcattaatg tgcgcaggat gtgaacagat ttttgaggac aaaatgagcg gaaccaagtc
      241 ggaacgacca ggcctgaaac gcgctttaaa gtgccttaaa agaggggata cattggtggt
      301 ctggaagctg gatcggttgg gtagaagaat gaaacacctc attgctctca cagaagagct
      361 acgcgcaaaa ggtgtcaatt ttcgcagtct gacggattca atcgatacca gcactccgat
      421 gggaaggttc ttttttcata tgatgggtgc actggcagaa atggagcgtg aactgatagt
      481 tgagcgcacg ctggctggac tggctgctgc gcgagcacaa ggaagagttg gaggacgtcg
      541 cccgaaactg acgaaggagc agcatgagca gatcgcaaga ttgctccaga aagggtatga
      601 cagaaagcgg ctggcaatta tctatgatat tggactgtca acgatctacc gctatcaccc
      661 tgttgggact gtcataacgc aacctgaaat gtaattcttt ttcaaataat gaaacgccgc
      721 gtggatgcca tttatcgcac aagatagagt gcatttatcg cgcggcgcat cattcttcat
      781 gtgaatcatc cgttgattaa ttatatataa acattatgta tcaacaagtt acattgataa
      841 ttaacgctac cattaataaa aataccccac aaggtccgaa aaaaaacgcc ggcaatgtgc
      901 cgacgtcttt gaaaagaaag gattgtctat gttatcgccc gttattcatt cagccgggtt
      961 gacttatccg cgccaattgc tcgaaatagt ctggaaaagt tttagcggta cattttggat
     1021 caagaatcgt cacaggcgta tctgataatg cgaccagcga gaaacacatc gccatccggt
     1081 gatcattata agtggcgatc tcagcaaatt tcagtttttc cggtggcgta atgcgaatga
     1141 aatcatgccc ctcttcgact tccgcaccga ctttacgcag ttctgttgcc atcgcaaaca
     1201 ggcgatcggt ctctttaaca cgccagttat agatattgcg cagcgtggtg gtgccttttg
     1261 caaataacgc cgcggtggca atggtcatcg ccgcatcggg aatatggttc atatccatat
     1321 caatggcgtt cagttcacca cgcgtgcagg aaatataatc atcgccccag caaatggtcg
     1381 cgcccatttt ttccagaaca tcagcaaagc gaatatcacc ctgcatactg ttgcgtccaa
     1441 tgccggtcac ttttacagtg ccgcctctga ttgctgctgc tgccaggaaa taagaagccg
     1501 aagatgcatc gccttcaacc aaataagtgc ccggagactg gtaagactgc ccccctttta
     1561 cgacaaattg ttgatagtgc tgattttcaa tttcaacacc aaacgtcttc atcagattga
     1621 gggtgatatc gatataaggt ttagaaacca gatcgccttt aatacgaatc accgtatctt
     1681 ccggcgcaag aggcgcagtc attaacagtg cggtgaggaa ctggctggaa acggagccat
     1741 caacatcaac gttgccgcca gtaaagccgc cctgtaaacg caatggcgga taattttctt
     1801 gttccaggta agtgatcttc gccccgccct ggcgcagcgc atccaccaga tgaccaatcg
     1861 ggcgttcttt cattcgcggt tcaccgctca gtacaatatc attgctaccc aggcaaagtg
     1921 ctgccgccag cggacgcatt gccgttccgg cgttaccgag gaacaactcc cgggcacttt
     1981 ctgcgtgtaa tggaccaccg ttaccgataa tttcgcaacg cgtacgatcg gctgaaagcg
     2041 tatagcttac ccctaacgct gttaatgcat tcagcatatg gcgcacgtca tcgctatcca
     2101 gcagattggt taatactgtt ttgccgtgtg ctaatgccgc cagcaataaa gcgcggttag
     2161 aaacgctctt ggaaccgggc agattaatag tgccatcgac acgagcgatg ggttgtaacg
     2221 ttagggattc catgaactca actctctaca acagaaataa aaaccccaca ggctggctgt
     2281 ggggattatg caaaatttcg acattagccg tgacgacgtt cgaactcaac catgaagtct
     2341 gtcagtgctt taacaccttc cagcggcatg gcgttataaa tagacgcacg cattccgccg
     2401 accactcggt gacctttcag cgcatgtaag cccgcagcga aagactcttc gaggaacaat
     2461 ttatcaagcg cgctatccgc caactggaac ggcacgttca tccgcgaacg gttagctttc
     2521 gccacatcat tgcggtagaa atcgctgtta tcaatcaccc catacagcag ttctgctttt
     2581 tgctgattga tcttatccat tgcagccaca ccgccgttcg ctttcagcca tttgaagacc
     2641 aggccagata agtaccaggc aaatgtcggc ggcgtgttga acatggaatc gttatcgttg
     2701 aggatggagt aatccagaat cgatgggcag gcgatattcg ctttgcccag caaatcttca
     2761 cgaacgatga cgattgtcag gccagccggg ccgatatttt tctgcgcgcc agcgtaaatt
     2821 acaccataac ggctgacatc aatcggacgg gaaagaatgg ttgaagagaa gtcagcggcg
     2881 accaccacgt ctttaccgaa gtctggtgtt tcatcgatag cgataccatc gatggtttcg
     2941 ttcgggcagt aatgcatata agcagcatta tcagagagtt gccattcact cattggctta
     3001 actgcgcgaa gaccatcaac ggtcactttc gcatcaaaca catttggtgt gcagtatttt
     3061 ttcgcttctt tgatagcact tgctgcccag taacccgcat caacataatc tgcagtggtt
     3121 ttatcaccaa gaatattcaa cggtactgca gcaaattgac cgcgaccacc gccatggcag
     3181 aacaatactt tgtagttgga ggggacgtta agaagatcgc gaaaatcctt ctcggcttcc
     3241 tctgcaacct gaatgaactc tttgccacgg tgactcactt ccatcaccga cgtaccaaga
     3301 ccgttccagt cgcgcagttc ctgttgagcc tgtttaagca cctctgccgg tagcattgcc
     3361 ggaccagaac taaaattgaa gatttgagcc atttcccctc accacgttgc gttgctatta
     3421 acccgcggtc aatcgattgc gaccacaggt aatacattat tccagtggct atcggtttta
     3481 tcattcagtg acacacatcg caatgagtaa aaccgttgag ggcaaaaatg tggcttgcat
     3541 cacacaaaag aatctctcgg attattagca aaaagccgat gtttttgctg tcaggtcacc
     3601 cgtttagctc gacttgcctt cgtccattct ggatttgcac agcgcggaca taattgtttt
     3661 tcccccgcgt tcatatgaat gatttcactg caacggatgc aggcatagtc cgccgactcc
     3721 ggcaccactt tgtaacgctg agtacagtca ctgggtaaaa taagtaaccc cggtttcacc
     3781 ttgtcatctt caaagaaata aacactgatt tgcccgttgg tttcgagaat cgccagacgt
     3841 acctgcccca actgctctac accgcgcaat cgtagctcca taaagaactc aaattccgtc
     3901 atgttggagt tattgagttt cgaccaggcc agctcgccgt cttcaataat gacaaccggc
     3961 ttgccttcca gaagatcttc cagtttttca ctgtgcgcca tcaaccacat taccaggcgg
     4021 tataacaacg ccagagtaat aaagacgata agtaccggga ccatcggcac atcatcataa
     4081 aacgccacat ctcccgccgc tgaacccagt gtcagaatga ttaacacttc aaacagcgac
     4141 atctgccgca caccgcgtct tccggtcatt ttgaggaaca aaaaaaccag tacaaaggta
     4201 taaagactac gtagtgcaac ttcgccaagg aaatcaaaag gcactttatc aaatgccata
     4261 cggtggagat cgaacgcctt catttttatg ctcttatcgt ggtttatccg ctaaaagcat
     4321 agccaacagt tcatcggcat ggcggcggtg atagcgcctt tgagataaaa cccgtattat
     4381 tgcgcgcttt ccgtacgact aaagtgattt tcatgacgca aacatttatc cccggcaaag
     4441 atgccgctct ggaagattcc atcgctcgct tccagcaaaa actttcagac ctcggttttc
     4501 agattgaaga ggcctcctgg ctgaatcccg tgcctaatgt ctggtctgta catattcgcg
     4561 acaaagagtg cgcactgtgt tttaccaacg gtaaaggcgc aaccaaaaaa gcggcgctgg
     4621 cttctgccct cggtgaatat ttcgagcgtc tctcaaccaa ctactttttt gcggacttct
     4681 ggctgggcga aaccatcgcc aacggcccat tcgtgcatta tcccaacgaa aaatggttcc
     4741 cactgaccga aaatgacgat gtaccagaag ggctgctcga tgaccgtctg cgcgcatttt
     4801 acgatccgga gaatgaactg accggtagta tgctgattga cctgcaatcc ggtaacgaag
     4861 atcgtggtat ttgcggtcta ccgtttacgc gccagtccga caatcagacc atttatattc
     4921 cgatgaatat cattggtaac ttgtacgttt ctaacggtat gtctgctggc aatacccgta
     4981 acgaagcacg cgttcagggg ttatccgaag ttttcgaacg ttacgtgaaa aaccgcatta
     5041 ttgctgaaag catcagcttg ccggaaatcc cggcagacgt gctggcacgt tatccagcag
     5101 tagttgaagc gatcgaaacg ctggaagcgg aaggtttccc gatcttcgct tatgacggtt
     5161 cgcttggcgg ccagtatccg gtgatttgcg tggtactgtt caatcctgct aacggtacct
     5221 gctttgcctc attcggtgcg catcctgatt ttggcgttgc actggaacgt accgtgaccg
     5281 agttgctgca aggtcgtggc ctgaaagatc tggatgtgtt tactccacca accttcgatg
     5341 atgaagaagt cgctgaacat accaacctcg aaacgcactt tatcgattcc agcggtttaa
     5401 tctcctggga cctgttcaag caggatgccg attatccgtt tgttgactgg agcttctccg
     5461 gcaccacgga agaagagttc gccacgctga tggctatctt caaaaaggaa gataaagaag
     5521 tttatattgc cgattacgag catctgggtg tctactcttg ccgtattatc gtacctggca
     5581 tgtccgatat ttatccggct gaagatctgt ggttggcgaa taacagtatg ggcagccatt
     5641 tacgtgaaac gattctgtca ctgcccggta gcgagtggga aaaggaagat tacctgaacc
     5701 tcatcgagca actggatgaa gaaggttttg atgactttac ccgcgtgcgt gaactgttgg
     5761 gcctggcgac cgggtcggat aacggctggt acactctgcg tatcggtgaa ttaaaagcca
     5821 tgctggcgct ggctggtggc gatctggaac aggctctggt ctggaccgaa tggacgatgg
     5881 agtttaactc atcagtattc agcccggaac gcgccaatta ttatcgctgc ctgcaaacat
     5941 tgttattact ggcgcaggaa gaagatcgcc agccgctgca atatctgaat gcgtttgttc
     6001 gcatgtacgg cgcagatgcc gtggaagccg ccagtgcagc aatgagcggc gaagcggcgt
     6061 tttatggctt gcaaccagta gatagcgatc tgcacgcgtt tgctgcacat cagtcgttgt
     6121 tgaaggccta cgaaaagctg caacgcgcca aagcagcatt ctgggcaaaa taaaatcaaa
     6181 tagcctacgc aatgtaggct taatgattcg tctgagttat attacggggc gtt
//
LOCUS       DACADA010000225         6165 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.227, whole genome shotgun sequence.
ACCESSION   DACADA010000225 DACADA010000000
VERSION     DACADA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.227"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(622..894)
                     /locus_tag="ISB17_004443"
     CDS             complement(622..894)
                     /locus_tag="ISB17_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAP1982303.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNG"
     gene            complement(1177..3864)
                     /locus_tag="ISB17_004444"
     CDS             complement(1177..3864)
                     /locus_tag="ISB17_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP1982304.1"
                     /translation="MKTPSVSTISGAAIGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEVAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKSLHGYSLPLLSQPLNLAGITFSTGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSEGWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKTLAEHLEQHVAH
                     YHGAPFRDWLYCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQSCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(3861..4271)
                     /locus_tag="ISB17_004445"
     CDS             complement(3861..4271)
                     /locus_tag="ISB17_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982305.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMVRVHNEPVYIEDGER
                     IELSHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(4264..4500)
                     /locus_tag="ISB17_004446"
     CDS             complement(4264..4500)
                     /locus_tag="ISB17_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982306.1"
                     /translation="MKSMNFKQGCILSEQGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGVMRDE"
     gene            complement(4497..5087)
                     /locus_tag="ISB17_004447"
     CDS             complement(4497..5087)
                     /locus_tag="ISB17_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1982307.1"
                     /translation="MPALINPGNDQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCYL
                     KAILHALGVFFCVAALTHLKFMVWWLIVFFYFLRHIIRIMVVQAGQLSGWPVSFRAGI
                     PTPVWATTHERRNSGGSVTRYLKEVAIMATVPALSHPEFTFVFLAVRRTDRDARPRPV
                     RVIAECEHAARLKLATEFILSFAARIPVKRAGEVVA"
     gene            complement(5175..>6165)
                     /locus_tag="ISB17_004448"
     CDS             complement(5175..>6165)
                     /locus_tag="ISB17_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HAP1982308.1"
                     /translation="TERPDAARRYHNKFVTGTNYQEESNRRFALSEPTENILRTSILE
                     SGWLIKNITLRDVNAVSGNAVNIGASELYTGRVDGGRFHKKMAINGTEFFLTETDTCA
                     RISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEINKKGE
                     DVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDALANHLITELIAEQFREDPRLV
                     VLVGAELAAHQRLKLFNAADRPSDVNAAQMATSSVAGRFAFIPPFMPGKRLAVTTLDN
                     LHIYTQAITRWFRAEFDDESSEYVHSYLRNEGYALGEPELYAAVDECTLTFAD"
BASE COUNT         1532 a         1710 c         1332 g         1591 t
ORIGIN      
        1 agttactacc tgtacatact aatcaaaaaa aacgagtacg caagatacga aagtactttt
       61 cttgttcacc agttagctta ccttgatcct caccagcgac tggaagatag gtagcagtat
      121 atgtggaaac cttcccctag ctaggtctac aaacgtagtt tgggcattta ggctccacga
      181 ttcacggttt gacacggcct gatatctgca cattcgtttt gatatgcagc aatcgtgaaa
      241 aaggaaaata tacgctcgac agaccaaaac ggcactacac cgcacccgcc tgcggttttc
      301 atatcactaa aaattttcag cctggatttt ttacaaacga taccgccagt ccgtgccagt
      361 cctcgagatg tactgcgata caggaactga aaagaatgaa aagaaattca ttttttttca
      421 gttttactgt ccgtcgaatc tatggcctga atacgcaact ctatgatatt aaatggataa
      481 ttaattttag gtgtgtgatt ttatgaaggt ttgtgaaaca tgaagaagat aaccagttga
      541 aaaataataa ttttatgacc gtataaactg aaatcctgtg ttcaggttcc gggaacagat
      601 cggccattcc cgacgcatac tttacccatt cttttgcgca gccagcagca actttaaagc
      661 cttttcccgt tcctcgggtg gaattgcatc aatcagctct ttaacccttc cctgcccgtt
      721 cagcgcgctt ggactaacgg aatgggagaa agcaacattc atcacaaacg agtgaccgca
      781 ctccacatta gagcaatagc agtacacatc gtacagttgt gggtgtttcc tgtttgtttt
      841 acctatccgt gctactgcct gacatgtggg gcagtacatt ctcatcatct tcatcgttct
      901 gtgccctata ctgttgcccg ccagaagaag ggatttggat ccctacctct tgttctgaac
      961 gtgtaacact ggtaacacgt gtaacgctat tgatttaaaa ggatttaatc tgttaccagt
     1021 tagcaaaatc aagaggtaac gcgtggtaac acaccatttt ttgttacctc tgttaccagt
     1081 cagtattttt gactggtaac actgaagaac cgcatcagta acgggtgtta cctctgttac
     1141 cagtgttacc tcataaaaat aagactcacg cgataatcac tcaggttcag cgcttcccag
     1201 tacctgtgta ttgaactggt acacacgttt aagccctatt tctggcaggc gaatgctgtt
     1261 ttgcgtccgg ccatcttcac cgggtttcag ccagcccgcg tccacacaca acctggccac
     1321 cttacgcgag tcaaatccct tacagatctc cttccagcct gacgggagaa cgtagaacgt
     1381 cacaaccggc tctgtcacgt tgtctccttt atcgactttt ctgaatccca tcattgaaac
     1441 aggccggttc ctgtcgtcat tccagtcagc aaaccgactg aactgattac gcgtcatgaa
     1501 gtcccgaacc tgttcaagtg ccgctttatc ttcctgatta gctgtatggc cccggtcagc
     1561 catccatgca gccagacagc tttgagcggc acgaaaggcc tctccctccg gccatcctgt
     1621 gatgcccgct tttgtggcca gctccccggc catcgcaacc agagcaaaac gggtaacagc
     1681 ccggccaacc tgattcccgg cattttccgg cgtcagcctt cgggtatact ctttcaataa
     1741 ggctttcgcc tgactggtca gttccggcag atcagcggtc aggcaataca gccagtcgcg
     1801 gaatggtgca ccatgataat gcgctacgtg ctgctcaaga tgctcagcca gggttttccc
     1861 gctactgaag ccatgaagtt cttcaaacac gccatacttg cccgaatcgc ttgggatctg
     1921 gatcattctg acctcaaccc cggcatacgt acgttccccg gcgcttgccg catgctctac
     1981 cagtgacagt tccccggtag acagaaacaa cagattccag cggttggttt cccttaccga
     2041 accatctgtt ctggctctgg ctttgccctg cccgttagcc agcatataag cgatatttcc
     2101 cgcctccctg ccgtcaacct cccggatttc atccagcatc agcgtggcat cattgcggcg
     2161 gctcgccgtt ccttctaagg cattccccgt cgcccgccac gtatgccaga aatctgttcc
     2221 accacatact gatgcagcca ctttcatcgt cgtggtttta ccgtctgtgg attccccttt
     2281 gaggtgataa cctccaccgc cgataccaac cagtttcaga aggggggcag caaatgccag
     2341 actcaccgca aaggcaagac gggcattctt tatgcaataa cggcctatat tttcacgcca
     2401 gccctctgat gtgccgctga cacgaaaatc acgcccctgc acactcgatg tttgtaagat
     2461 gactgactgg gcttcgcgcc ctatcacttc atcctggaga acgtaaaccc cgccgtgcca
     2521 gccagtttta ttcacacagg ttacttttct gtccggttta cacagcgaga tatattccat
     2581 caggaatgct ctggccatgc cgttaatgtt gatgtaagaa agcccgttaa ccagcagaac
     2641 gcggcgcagt tcttccccgc taccgcccag catctccatc ggcattgccc atttacggct
     2701 gtttccgttg gtatcttccc attccagcaa acgcccgtaa ttacttccat cagcatcact
     2761 ggtgattgcc gtcacccgca acggactgca aattttgata ttacgtatct ctgtatcacc
     2821 atcagactta ttaaccagct tgtcgtacca caaatattct tttgtcagcc ggaacccttc
     2881 tggtaaacgg gtctgccctt tgccatgcag cgtcatttct tcacgaaagg catctctcgc
     2941 ccgttctttc cccacttcct gccgataatc atcccagtca gccttatggc gtaccggagg
     3001 gagcgttacc cagccatcca ccgctttggc ggctttctct gcctgaatac gcccggtgtt
     3061 ttctttgccg tcagccagat cattatctcc ggccagaatg atccgggttt ccggccagcg
     3121 ttttcggatc tgctccgtca ccttcagtaa attggtggca gcgacagccg ccacgatcca
     3181 tccttcagtc agcagactga cggtaagcgc cgtggcaaaa ccctcagtga taatcacctg
     3241 ttcaggtgtt tcagcgggta tatcggtcag ggcaatgaat gcgccagaca gctggctacc
     3301 gggcagcaga cttttatccc cgtcaggatt gataagctgg ccaccagtaa tattcccgga
     3361 aatatccgtc aaaggcagca gtaatgaacc ggtggaaaat gttattccgg ccagattcaa
     3421 aggctgtgac agcaagggaa gtgaataccc gtgtaagctc ttatttgtca gatagaccgg
     3481 ctcaccattg caggactgct gtctgagtct ggtatatcgc tccgcgcccg cttcttttcc
     3541 tgcggccttt ttcctggctg gcaatgcggg cttctcctgt atttctggta atgcaagcgc
     3601 ctcagatacc atcccggcga cttcttttat ctttcttcct gtcaccagac gaaccagatc
     3661 aagaccatcg ccatgaccgc actgattaca aaaccacgtc cctcgccccg ctttatcgtc
     3721 cagtcgaaaa cgatcttttc caccacaggc agggcaagcc ccatgatgtc ctgcggacgg
     3781 tactttaata cctaatgctg acaggatata aggccagcgc ccaattgcag ctccggaaat
     3841 cgtggataca gacggtgttt tcacagcagc acctcctgac cttccggctt cacaaattca
     3901 aaaccttcgg gtagttcgcc atcaggtgct ccgaaaataa tatcccggta aacctcacgc
     3961 atctctgtga caccaaaaac tgacaggcga atattttctg acataaagcc tggtttaagc
     4021 attttttcca gtttctctgc ggccagtatg caaccagcat cagccccaaa ctcattcacc
     4081 cacggcattt caatatggta aataatatta ctcaccactt tttcgtggga gagttctatt
     4141 cgttcgccat cttcaatata aacaggttca ttatgaacac gcaccattaa ggaaatgaat
     4201 gcatcagcaa tgtagtagcg taagactgct gttcgtaaag catgcgcctt taaattttga
     4261 ttattattca tcgcgcatca ctcccttaat tgtgctggtt tccttaacaa cgccatccag
     4321 ttgttcggca atacaggcca ctaaagccgc agcgccttcc gctgaaacag ttctcgctcc
     4381 attcactgtg ggcacggaca agacctctgc aagaaactca ctggccagtg ccgcccgtaa
     4441 cagacgactc tccccctgtt cactcaatat gcagccttgc ttaaaattca tggatttcat
     4501 gcaaccacct ccccggctct tttcacaggg atacgggcag caaagctcag gataaattca
     4561 gtggcaagtt taaggcgtgc agcatgctcg cattctgcga ttacacgcac agggcgtgga
     4621 cgggcatcac ggtctgtacg gcggacagcc agaaagacaa aggtaaattc agggtgagat
     4681 aaagcaggga ctgtagccat aatggcaacc tccttcagat agcgggtaac gctaccaccg
     4741 gagttcctac gctcatgggt ggtagcccag acgggggtag gaataccggc tctgaaggat
     4801 accggccagc ccgaaagctg ccccgcctgg actaccataa ttctgatgat gtggcgtaaa
     4861 aaataaaaaa atacaatcag ccaccacacc ataaatttta ggtgagtcaa agctgcgaca
     4921 caaaaaaaca cgcctagcgc gtgtagtatc gccttcagat aacacgggtt cctacgcccg
     4981 gctgccgatt ttgcggcaac ctttaaacta tatcccgcgc attcctggtg aggcaagaaa
     5041 tttataaccg aaaactgatc attgcctgga ttaattaacg ctggcatgct taagcctctg
     5101 cttaaaaata gttcttcatt tttcctgatg ctgtaaacga gacccgcctc tgagcgggtc
     5161 tttctgtcgc taagtcaatc cgcaaacgtc agggtgcact catcaacagc cgcgtacagt
     5221 tcaggttcac cgagtgcata accttcattg cgcaggtagg aatgcacgta ttcactgctt
     5281 tcatcatcaa actcagcacg aaaccagcgt gttatcgcct gagtgtaaat atgcagatta
     5341 tccagcgtcg ttactgccag acgcttaccc ggcataaacg ggggaataaa cgcaaagcgg
     5401 ccagcaacag aacttgtagc catctgtgcc gcattcacat cagacgggcg gtctgcggca
     5461 ttaaataact tcaggcgttg atgtgctgcc agctctgccc caaccagtac aaccagacga
     5521 ggatcttcac gaaactgctc agcaataagc tctgtaatca gatggttggc cagagcatca
     5581 atatttttcc aggtgccagt ctctcccaga gtgacaggct cagagatgat ttgctttccg
     5641 ttcccgtagg ctttcgccag tgcatgccag ccaatattga catcctcgcc ttttttattg
     5701 atttccgggt ttgtagtatt tgcaatcgac acaccgttga aaccgacccg cagcatgtcc
     5761 agcgcatatg cctgggcaaa aaagctctca accgttttct caaactcatc cggcacgccg
     5821 gtatggtaaa tatgagacat atcggaatag ctgatccggg cgcaggtatc cgtctctgtc
     5881 agaaagaact ccgtaccatt gattgccatt tttttgtgga aacggccacc atccacacgt
     5941 ccggtataca gttcactggc accaatattg acggcattac ctgacacggc gttaacatca
     6001 cgtagggtaa tatttttaat aagccacccg gattccagaa ttgaggtacg cagtatattt
     6061 tcagtaggtt cgcttaatgc aaagcgtctg tttgattctt cctgatagtt agtgccagtg
     6121 acgaatttat tatgataacg acgtgctgcg tccggccttt cagtt
//
LOCUS       DACADA010000226         6133 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.228, whole genome shotgun sequence.
ACCESSION   DACADA010000226 DACADA010000000
VERSION     DACADA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.228"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            154..882
                     /gene="gadW"
                     /locus_tag="ISB17_004449"
     CDS             154..882
                     /gene="gadW"
                     /locus_tag="ISB17_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAP1982309.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1245..4358)
                     /locus_tag="ISB17_004450"
     CDS             complement(1245..4358)
                     /locus_tag="ISB17_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP1982310.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4383..5540)
                     /gene="mdtE"
                     /locus_tag="ISB17_004451"
     CDS             complement(4383..5540)
                     /gene="mdtE"
                     /locus_tag="ISB17_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAP1982311.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5600..5929
                     /locus_tag="ISB17_004452"
     CDS             5600..5929
                     /locus_tag="ISB17_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982312.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5879..>6133)
                     /gene="gadE"
                     /locus_tag="ISB17_004453"
     CDS             complement(5879..>6133)
                     /gene="gadE"
                     /locus_tag="ISB17_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAP1982313.1"
                     /translation="KESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESN
                     ITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1510 a         1513 c         1529 g         1581 t
ORIGIN      
        1 cttattatca attaactgtt atgcaaaact actttgtgga taaaatttgg tcctaccaaa
       61 tatggcagtt tttgcacaca gaaacagtct ggcgtcattt cattagtata ctgacattga
      121 aataatcgca gtaatgaaat ataagggata gtcatggctc atgtctgctc ggtgatcctc
      181 gttcgtcgtt cattcgatat tcatcatgaa cagcaaaaaa tatcgttgca taacgagagt
      241 atcctgctgc tggataaaaa tttggcagac gattttgcgt tttgttcact ggatacgcga
      301 cggctggata tcgaagagct gacagtttgc cattacttac aaaatattcg tcagttgcca
      361 cgcaatttag gattgcatag caaagaccgt ttgttaatta accagtcacc ccccatacag
      421 ctggtgacgg cgatttttga tagtttcaat gacccccggg tcaattcgcc gatactgagc
      481 aaaatgctct atctttcctg tttatcaatg ttttctcata agaaagaact gatcccctta
      541 cttttcaata gtatcagtac tgtttcagga aaagttgaac gccttattag ctttgatatc
      601 gctaaacgtt ggtatctacg cgatatcgca gaaagaatgt acaccagcga gagtctcatc
      661 aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa tattactcgc ctccaggatg
      721 tcgatggcca gacgattact cgagttacgt caaatacctc tgcatactat tgcggaaaaa
      781 tgtggctata gcagtacgtc atactttata aatacattta gacaatatta tggtgtaacg
      841 ccacatcagt tttcgcaaca ttcgccgggt accttttcct gacatatttc gcatttgaat
      901 attggtcagg atctcacgtc tgcttcatgt gaaactcttt cctgatgatt tctgccgggc
      961 taccggctag ttctctttcg cagtttgcca ggcggtatag gtattttcag gaggaaaaga
     1021 ctcggcatgt ttgggattat taagctaaca attcatacga ttacgggaat gtgggtcagt
     1081 attgtgctgt tcaaattgat gacgaatggc tggagtggtt tctactttca gtgctgtgta
     1141 ctaagcctgg tattcctgac ggttagctgg ttgttgagtg gtgaatggtt agcaggaaag
     1201 agtaaggctg aaccctcacg ttcaacctta ctctcattta cacgttacgc ttttttaaag
     1261 cgggcaaaga gatgttccac tacaacgaaa aagaccggaa cgaagtaaat tgccagcact
     1321 gttgcggcaa acatcccgcc cattacgccg gtacctaccg cgttttgcgc gccagagccg
     1381 gcaccatgac tgataaccag cggcagcacg ccgagaataa aggccagaga agtcatcagg
     1441 attgggcgta aacgcatacg cgctgcttcg atgattgcct ctaccggtgt tttcccttct
     1501 ttctgcatca tctcaacggc aaattcgaca atcaggatgg cgttcttggc ggaaagcccg
     1561 atggtggtca gcaaaccaac ctggaagtaa acgtcattac ttaagccgcg cagatcggtg
     1621 gccagtaatg cgccaacgac gcctaacgga acaaccaaca tcaccgagaa cggaattgac
     1681 cagctttcat aaagtgcggc gagggcgagg aacaccacga ccagtgaaat ggcatatagc
     1741 gcgggagcct gatttgagga taacgcttcc tgatacgaca gtccggtcca tgagtagccg
     1801 acgcctgccg gaagttttgc gaccaggtct gccataaatt tcatggcgtc accggtactt
     1861 ttcccggcag ccgcttcacc taaaatctcc attgacggga tgccgttgta gcgttccagt
     1921 cgcggtgaac cataggtcca ttcggtagac gaataggcag aaagcggtgc catcgtgcca
     1981 gaggcgttgc gtacatacca ttggttgatg ttatccggca acatacggaa cggcgtgcct
     2041 gcctggacat acactttttt cacccgcccc tggttgagga agtcgttcac gtagctgctg
     2101 ccgaaggcgg tggaaattgt ctggttgata tcagacagcg ccacacccat agcctcggct
     2161 ttcgcagcgt tgacgttcac tttgaacatc ggcgtatctt ccaggccgtt cgggcgtacc
     2221 ccgatgacct gattcggtga ttgcgctgcc agtgataaca gctcgtttcg cgcctgggtc
     2281 agtttttcgt gccccagatt gccgttgtcc agcagttcca tatcaaaacc tgacgcggta
     2341 cccagttcag ccaccgcggg taagttgaac gggaagacga cggctttatt gatactgctt
     2401 agcgcaatca ttgcccgctg aataatcgcg gtaaccgagt tttcctcgcc gacacgttca
     2461 gaccacggct tgaggctgat aaacgccaga ccgttgtttt gcccctgacc actgaagcca
     2521 aagccaccaa cggtaaacac cgactgaaca ttatttttct cttttgtcag ataataatcc
     2581 gtcacctgtt gtagcacttt cgtggtgtta accatggttg caccggaagg taactgcgcg
     2641 gtggtcataa ataccccctg atcctcttct ggtaagaaag aggtcggcgt acgcaggaac
     2701 agcaccgcca tcccggcgca aatcagtagg tagaccacca tgtagcgacc ggtacaacgc
     2761 aacagcgagc gggtgctgtc ggtatagtgc tgagttgatt tttcaaacag cgtgttgaag
     2821 cgtgcgaaca gggcgttagg tttgtgaccg ccctccggcg cggctttcag aatggtggcg
     2881 cacagggcag gggtcaggct cattgctaca aatactgaaa gcagcatgga ggagatcagc
     2941 gtgatggaga actggcggta gatctccccg gtcgcaccgc tcataaaggc catcggcata
     3001 aacactgcgg aaagaacaac ggcaatcccg accagcgcac gttggatctg ccccatcgat
     3061 ttatgcgtcg cttccttcgg cggtagctta tcttccgcaa tgacacgctc gacgttctcc
     3121 accaccacga tggcgtcatc caccagtaac cctatcgcca gcaccatccc gaacatagtt
     3181 aacgtgttga tggtgaaacc aactgccgac aagatcgcaa acgtcccgag aataaccacc
     3241 ggtacggcaa tcgtcgggat gattgtggcg cggaaattct gcaaaaacag atacatgacc
     3301 aggaagacga ggatgatagc ctcaaccagc gttttgaaaa cttcctgaat agaaattttg
     3361 ataaacggtg tggtgtcgta aggataaacc gtcttcaggc ttgcagggaa ataagccgat
     3421 aaacggttca gctcctcttt caccgctcgc gaggtatcca gggcgtttgc tccggtagcc
     3481 agtttgatgg cgatcccggc ggcaggtttg ccgttatagc gcgccacggt ggaataatct
     3541 tccgccccaa gttcgacgcg ggcgacatca cgcagcagca cttgcgaacc atcttgctga
     3601 actttcaaca ggattttgcc aaattcttcc ggcgtttgca gacgcgtctg cacaatgatc
     3661 gaggcgttca gttgctggtc tgccgcctgt ggcatgccac ccagttgacc accagaaatc
     3721 tggttgtttt gcaccttaat ctgggaaata acatcggaag gtaccaggtt atatttattg
     3781 agtttttgcg ggtccagcca gatacgcatg gcgtactcgg aaccaaagag ctgtacgcta
     3841 ccaacgcccg cggtacggct tagcgggtct ttgatattag acgctacata gtccgcgata
     3901 tcgtactggt tgaggctgcc gttatcagaa ataaatgccg ctaccatcag gatattactg
     3961 ctcgacttat cgacgctaat cccctgctgc tgcaccgctt caggtaatga aggcatagcg
     4021 agttgcagtt tattttgcac ctgaacctgt gcgatatcag gcgatgtccc cgtctcgaag
     4081 gtcagagtga tggaggcatt acctgccgca tcgctggttg aagacatgta catcaggcca
     4141 tcaagcccat tcatattttg ctcaatcacc tgagtgaccg agtcttctac cgtttgcgca
     4201 tcggcacccg gataggttgc gctgacggta atggtcggtg gcgcaatctg cggatactgc
     4261 gcaaccggta agttcatgat cgccagaccg cctgcaagca tcataataat ggcaagtacc
     4321 caggcaaaaa ccgggcgatc aataaaatag ttagccatga aagtcccctt aagcctgcaa
     4381 cgttattgtt tcgattcggt gctggcgttt tcctggctgg aggaaattgc tcgtgctttg
     4441 atacccggac gaatgcgttg caagccggaa acgatcaccc gatcgccagc ctgcaagcca
     4501 gaggtgacaa cccactggtc gccgatggct ttgctggctt cgatttcgcg tagctgcacg
     4561 acatcgtctt tatccagaat gagtgccgtt gctttaccct gggcgttgtg ggtgacgcct
     4621 tcctgcggca ccagtaatac attctggcgg ctaccttcat ccactaatgc cgtaacatac
     4681 atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt cacggagccc
     4741 gtggtttcat ccaccgtcgg gtcggagaat ttcagcgtgc cggtctggcc gtaacgttta
     4801 ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat ttgcccactg
     4861 gcgacctctt ctttcatgcg caagaaatcc tgcaccgact gcgtgagatc gacataaatc
     4921 gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg
     4981 acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc
     5041 agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc ttctgcttca
     5101 ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa catagttggt cttcagcaac
     5161 gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc tttcgccagc
     5221 gagcctttgg cggagttcag ctctgcctgt aaaggcgcag gatcaatctg atacagcgaa
     5281 tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat tccgcccacc
     5341 tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc gctcaacaca
     5401 ttgaccgaac cgggggagag tgtgacgaca ccgacctcag gcgtcatggc ggcggcgttt
     5461 tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa cggtattaac
     5521 agttttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac ggttctttaa
     5581 attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg ggttgtcagc
     5641 cagtaagatg ggatgcaagc acagtgatcg acatggtgag gtcaacgaca aaaaaaatgt
     5701 tgccgttctg ccaacagttt ctgccagcat tcgggcaacg aagggttcta ctggtggata
     5761 cacataccag gggaataaag tcgttttcgt ctctccttca ataatcaccg ggggtattga
     5821 aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta tgtccttact
     5881 aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttaat ttcagcttgt
     5941 acataatgtt gaatttctga atctttaatg tttgttgcga aatgttgagc gttgacgtga
     6001 tattgctttc atttttgttt ttcagaatca gttgcataat tttgaactga ttatgtgaaa
     6061 aacatagttg tgaattctta tggggcaagt gtttaccata agtaatctca agaagatttt
     6121 tgatactttc ttt
//
LOCUS       DACADA010000227         6116 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.229, whole genome shotgun sequence.
ACCESSION   DACADA010000227 DACADA010000000
VERSION     DACADA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.229"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            99..812
                     /locus_tag="ISB17_004454"
     CDS             99..812
                     /locus_tag="ISB17_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982314.1"
                     /translation="MRLKALAFLVPLEKISWIAGSERACLFSWYLLMQFIQNNRAKIS
                     ADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEWIIQTKDRWIR
                     AFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHAIHLSFDIWVT
                     CPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLKELSRVRRLST
                     GEMLHDLIVEEYKRYRHSR"
     gene            897..1769
                     /locus_tag="ISB17_004455"
     CDS             897..1769
                     /locus_tag="ISB17_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP1982315.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRNRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2142..4988
                     /gene="ag43"
                     /locus_tag="ISB17_004456"
     CDS             2142..4988
                     /gene="ag43"
                     /locus_tag="ISB17_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP1982316.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDADNGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGNADGVVLESGGRLDVLEGHSAQNTLVDDGGILAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGN
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARI
                     RENITLGVQGGYAHSVSGSSAEGYNGQATLNVTF"
     gene            5059..5217
                     /locus_tag="ISB17_004457"
     CDS             5059..5217
                     /locus_tag="ISB17_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1982317.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            5372..>6116
                     /locus_tag="ISB17_004458"
     CDS             5372..>6116
                     /locus_tag="ISB17_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1982318.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSPGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPTRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIH"
BASE COUNT         1487 a         1582 c         1708 g         1339 t
ORIGIN      
        1 atgatccccc ggaacgtttt atcgcttaca taaaaaaaaa cagagaaaat ctgaacatca
       61 cactttcaga ttttgaactt gaacaaataa tttctggtat gcgtctgaag gccctggcct
      121 tcctggtccc cctggaaaaa atatcctgga tcgcgggcag tgaacgcgca tgcttatttt
      181 cctggtatct gttgatgcag tttatccaga ataacagggc aaaaataagc gcagacttgc
      241 ttcaaaagaa taaactgtat cttaaagaag agtatctgga agggaatgcc ttcccgtcgg
      301 actcatcgac acagttcaga caaatactca gagttctcga tattctttct gataagaatt
      361 tgcgggatga gtggataatc cagaccaaag acagatggat acgggcattt aaatcaaaga
      421 gtcctttcag ttacctcctc ccggagaacg aacatgaatg catatggaca tggaattatt
      481 tgaagggaaa aaatattgca ctggaaaaac tggccagttt tcctggttct gctgatattt
      541 accacgcaat ccacctttca tttgacatat gggttacctg tccactcacc tcacctgacg
      601 atataaaaaa tttcagaaac agtttcaata aggccaaggc gcagcgcaag tacaaaaaaa
      661 tgcaggaaga taaagtcaat gtacagtttt ttcttgacgc agaaaccagg gcgcagctca
      721 aagaattatc gagggtcaga agactcagta ccggcgaaat gctgcatgac ctgattgttg
      781 aagagtataa aaggtaccga cattccagat aatttcagac atatattatc tccgtgagtc
      841 ccccgccacc tttccggtgt gcgggggttt tgtctttttt caccgggaat acatgtatga
      901 atccgtctga tgccattgag gcaattgaaa aaccgctctc ctccctgcct tactcgcttt
      961 cccgtcacat cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg attggcatta
     1021 tgggtaaaag cggggccggt aaatcctcac tctgtaatgc actgtttcag ggggaggtca
     1081 ccccggtcag tgatgttcac gccggcaccc gggaagtacg gcgcttccgt ctgagtggcc
     1141 atggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg aacagggatg
     1201 cagagtatga agccctgtac cgtgacattc tgcctgaact tgacctggta ctgtggctga
     1261 ttaaagccga tgaccgtgcc ctgtctgtgg atgagtattt ctggcgacac atcctgcacc
     1321 ggggacatca gcaggtgctg tttgtggtga cgcaggccga caaaacggag ccctgccatg
     1381 aatgggatat ggccggcatt cagccttctc ccgcacaggc acagaacatt cgggagaaaa
     1441 cggaggcggt attccgtctg ttccggcccg tacatccggt tgtggccgta tcggcccgca
     1501 ccggctggga actggatacg ctggtcagtg cgctcatgac agcacttccc gaccatgccg
     1561 ccagtcccct gatgacccgc ctgcaggacg agctgcgcac ggaatctgtc cggtctcagg
     1621 cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacggcggag agcgtctgta
     1681 ttgcctctgt tgcacgcacg gttctgcgtg ccgtccgtga cacggtggtc tctgttgccc
     1741 gcgctgtatg gaactggatt ttcttctgaa cactctgcca ggggaaatgc ctgattacat
     1801 tgcgttaacc aactgaaaac atgatacaaa cactccatcc ccctcctgtt tctcttgcgt
     1861 gactgctctg ctgttaatag aataaaacga tcgataaaac aggtatcgat cgttaccatc
     1921 gatcgtttat agtggttaat ggtctgcgaa aaggcagctg aatctcttca tcatgcagaa
     1981 cggaattgca cacaacggac tgatacttct ctggcggatg agagagggga agcatttatg
     2041 tccgggaaaa accactttat ctgaccatgc tgttttgtac ctgccggtat ccacttttgt
     2101 gggtaccggc ttttttattc accctctgta aggaaaagct gatgaaacga catctgaaca
     2161 ccagctacag gctggtatgg aatcacatta cgggcaccct ggtggtggcc tccgaactgg
     2221 cccgctcacg gggaaaacgc gccggtgtgg cggttgcact gtctcttgct gctgtcacat
     2281 cagtcccggc actggctgct gacacggttg tacaggcggg agaaaccgtg aacgacggaa
     2341 cactgacaaa tcatgacaac cagattgtcc tcggtacggc caacggaatg accatcagta
     2401 ccgggctgga gtatgggccg gataacgagg ccaataccgg cggacaatgg atacaaaatg
     2461 gcggtatcgc caacaacact actgtcaccg gtggtggtct tcagagagtg aatgccggag
     2521 gaagcgtttc agacacggtt atcagtgccg gaggaggaca gagccttcag gggcaggcag
     2581 tgaataccac tctgaacggc ggtgagcagt gggtacatga aggcgggatt gcaacgggta
     2641 ccgtcattaa tgagaagggc tggcaggccg tcaaatccgg cgcaatggca accgacacgg
     2701 ttgtgaatac cggcgcggaa gggggaccag atgcagacaa tggtgatacc gggcagtttg
     2761 ttcgcggaaa tgccgtacgt accaccatca ataaaaatgg tcgtcagatt gtggctgctg
     2821 aaggaacagc aaataccact gtggtttatg ccggcggcga ccagacggta cacgggcatg
     2881 cgctggatac cacactgaat ggcggttacc agtatgtgca caacggaggc acagcctctg
     2941 acacggttgt aaacagtgac ggctggcaga ttgtcaagga aggtggtctg gcggatttca
     3001 ccaccgttaa ccagaaaggc aaactgcagg tgaacgccgg tggtacagcc acgaatgtca
     3061 ccctgaagca gggaggcgca ctggtcacca gtacggcggc aaccgtcacc ggcagcaacc
     3121 gtctgggcaa tttcactgtg gaaaacggta atgctgacgg tgttgttctg gagtccggtg
     3181 gtcgcctgga tgtactggaa ggccattcag cgcagaatac cctggtggat gacggcggta
     3241 tcctggcagt gtctgccggt ggtaaggcaa cagatgtcac catgacatcc ggtggtgccc
     3301 tgatagcaga cagtggtgcc actgttgagg ggaccaatgc cagcggtaag ttcagtattg
     3361 atggtatctc cggtcaggcc agcggcctgc tgctggaaaa tggcggcagc tttacggtta
     3421 atgccggagg acaggctggc aacaccactg tcggacatcg tggaacactg acgctggctg
     3481 ccgggggaaa tctgagtggc agaacacagc tcagtaaagg tgccagcatg gtactgaatg
     3541 gtgatgtggt cagtaccggc gatattgtta acgcagggga gattcgcttt gataatcaga
     3601 cgacaccgga tgccgcgctg agccgtgctg ttgcaaaagg cgactccccg gtaacgttcc
     3661 ataaactgac caccagtaac ctcaccggtc agggtggcac catcaatatg cgtgttcgcc
     3721 ttgatggcag caatacctct gaccagctgg tgattaatgg tggtcaggca accggcaaaa
     3781 cctggcttgc gtttacaaat gtcggaaaca gtaaccttgg ggtggcaacc tccggacagg
     3841 gtatccgggt tgtggatgca cagaatggcg ccaccacaga agaaggtgcg tttgccctga
     3901 gtcgcccgct tcaggccggc gcctttaact acaccctgaa ccgtgacagc gatgaagact
     3961 ggtacctgcg cagtgaaaat gcttatcgtg ctgaagtccc cctgtataca tccatgctga
     4021 cacaggcaat ggactatgac cggattctgg caggctcccg cagccatcag accggtgtaa
     4081 acggtgaaaa taacagcgtc cgtctcagca ttcagggcgg tcatctcggt cacgataaca
     4141 acggcggtat tgcccgtgga gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc
     4201 tggagggtga cctgctcaga acagaggttg ccggtatgtc tctgacgaca ggggtgtatg
     4261 gtgccgcagg ccattcttcc gttgatgtta aggatgatga cggctcccgc gccggcacgg
     4321 tccgggatga tgccggcagt ctgggcggat acctgaatct ggtacacaca tcctccggcc
     4381 tgtgggctga cattgtggcc cagggaaccc gtcacagcat gaaagcgtca tcggacaata
     4441 acgacttccg cgcccggggc tggggctggc tgggctcact ggaaaccggt ctgcccttca
     4501 gtatcactga caatctgatg ctggagccac aactgcagta cacctggcag ggactctccc
     4561 tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt gcacaacatg
     4621 tgcgtgccgg tttccgtctg ggcagccaca acgatatgag ctttggtgaa ggcacctcat
     4681 cccgtgacac cctgcgcgac agtgcaaaac acagtgtgag tgaactgccg gtgaactggt
     4741 gggtacagcc ttctgttatc cgcaccttca gctcccgggg tgacatgagc atggggacag
     4801 ccgcagccgg cagtaacatg acgttctcac cgtcccggaa tggcacgtca ctggacctgc
     4861 aggccggact ggaagcccgt atccgggaaa atatcaccct gggcgttcag ggcggttatg
     4921 cccacagcgt cagcggcagc agcgctgaag gttataacgg tcaggccacg ctgaatgtga
     4981 ctttctgata atcaggtcac tctccttcag gccccggtca tcatgaccgg ggctttttac
     5041 tttcagggag caacagatat gccgggttgt acatcacgtt tattgcccga aggccctttc
     5101 tcacgcgaac aggctgttgc cgtcaaaaca gcttaccgca atgtgtttac tgaagatgac
     5161 cagggaacat attcccggct ggttatccgt aatgctgaag gtcagttgcg ctggtgatgc
     5221 cggaacacag cttaatactc agctcgccag ttaagggctt ctcagacaat aaacgtcatt
     5281 catttcatct gtcaggccgc gtcttctccg ggagacgtgg ctttttcatt tattacacca
     5341 gtaaatctta accaccgata aggagcaaat tatgcgacta gccagtcgtt ttggttatac
     5401 gaaccagata cgccgtgacc gtccgctgac acacgaagaa ctgatgcact atgttcccag
     5461 tattttcggt gaagaccggc atacttcccg cagtaaacga tatgcgtaca ttcccaccat
     5521 caccgtactg gaaagcctgc agcgggaagg ctttcagcca ttcttcgcct gccagacccg
     5581 tgtgcgcgac ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg
     5641 agagataaac ggagaacatg tccctgaaat cattctgctc aactcccatg acggtacctc
     5701 cagctaccag atgctgccgg gttacttcag gttcgtctgt cagaacggat gcgtctgtgg
     5761 tcagtctccg ggggaagtgc gtgttccgca tcggggaaat gtggtggaca gagtcattga
     5821 aggggcttac gaggtggtgg gcgtgtttga ccggattgag gaaaagcgtg atgccatgca
     5881 gtcgctggtc ctgccgccac cgacacgcca ggcgctggca caggcggcac tgacttaccg
     5941 ttatggtgac gaacatcagc cagtcaccac cgctgacatt ctgacaccac gacgccggga
     6001 ggattacggt aaagacctgt ggagcgcata tcagaccatc caggagaata tgctgaaagg
     6061 cggaatttcc ggccgcagtg caaaaggaaa acgtatccac acccgtgcca ttcaca
//
LOCUS       DACADA010000228         5897 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.230, whole genome shotgun sequence.
ACCESSION   DACADA010000228 DACADA010000000
VERSION     DACADA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.230"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            167..748
                     /gene="mdaB"
                     /locus_tag="ISB17_004459"
     CDS             167..748
                     /gene="mdaB"
                     /locus_tag="ISB17_004459"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAP1982319.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            779..1093
                     /locus_tag="ISB17_004460"
     CDS             779..1093
                     /locus_tag="ISB17_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP1982320.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1138..2025)
                     /locus_tag="ISB17_004461"
     CDS             complement(1138..2025)
                     /locus_tag="ISB17_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1982321.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2022..2969)
                     /locus_tag="ISB17_004462"
     CDS             complement(2022..2969)
                     /locus_tag="ISB17_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1982322.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2980..4017)
                     /locus_tag="ISB17_004463"
     CDS             complement(2980..4017)
                     /locus_tag="ISB17_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1982323.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4026..5009)
                     /locus_tag="ISB17_004464"
     CDS             complement(4026..5009)
                     /locus_tag="ISB17_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1982324.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5006..5815)
                     /locus_tag="ISB17_004465"
     CDS             complement(5006..5815)
                     /locus_tag="ISB17_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1982325.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1664 a         1599 c         1367 g         1267 t
ORIGIN      
        1 gatttaagta ttgtcatttt cagaaaatat ttatcccgca tcggtgaatc agagaaagat
       61 cagacttttg ctaaattcgc aaaagacttt gcacattttg ctaatttcac cgtaccgctc
      121 tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt aaaaaaatga gcaacatcct
      181 gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac
      241 cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcattg ttcgcgccga
      301 cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg
      361 gcagatgcct ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt
      421 attcaccgaa ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccatc
      481 aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt ctctgacctg
      541 gaacgcacca atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggtgttga
      601 cggtgtgtac ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac cgctgccgac
      661 atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg
      721 caagcatctt gtggaaattt ttggttaact agagctctgg ctttagaagg agttaaccat
      781 gattacagta atcgcagaaa ttcgtactcg tcctggtcaa catcaccgtc aggcagtgtt
      841 ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc
      901 gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt
      961 gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa ccccgcacat
     1021 gaaagcatat agcgaagccg taaaaggtga cgtgctggag atgaatattc gtatcctgca
     1081 gccggggctt tgacatttct aaaaagaggc cggtgctgct accggtctca gcccagatta
     1141 gccattcttt cttcccgttt tcagtcctcg atggaaatcg ttaattcgct taatggtttt
     1201 aaccgcacgc tgcggcactg cggaagctac cgaccagaca atcatttcca gacacaacaa
     1261 taccgtacca tgtagcggcg ttttcccttt ttcatccccg cgcggaacat gaatcacgat
     1321 actggcgtct ttgctaaaac gtgaatccag cgcgtttgtc agcaaaataa cagggatccc
     1381 cagccgcctc gcctcacgaa gcgttgtcag cccttcccgg tgcgcagatt tttgcgccat
     1441 catgatcaga acatcgccac gttgaagcgc gatcagttgc tcggcaaggc caatacccgt
     1501 acggtttagt gccgtggcag gcaaacctat gcggttgaat agtcgtgcgg tatagtccgc
     1561 cagaataccc gaagcgccaa tgccaaaaat tgcgacttgc cgtgcctgca ccaacaacgc
     1621 tacagcttgc gcaagcgcgt ggcggttttc aggctctgac aggacattac aggtatacaa
     1681 atgcccttca agaacaaagt cgattgcggt gttaacatcg ctcgtcaagt tactcaccgt
     1741 cgtagacatc ttttcgctgg aactaagggc tggaccaagc cactgttcca gagtgcgttt
     1801 cagatcacgc agcccggcaa accccagggc ctgtatagcc ctgataactg tggcatcgga
     1861 agtgttcaac gtggccgcaa tttccatcgc cgtttgctcg agtaccactt cacgatgctc
     1921 attaatgtag ctggcgacaa ctcttaaccc aggcgttaac tgactcgcgc gagtgcgaaa
     1981 acgctcacca aaaatatcaa cccgcacttt atctttctgg atcatttagc ctccggtaga
     2041 ggtcgccctg caatttgtga ttgtatctgc gcagccatca gccccagaga tgcaaaggag
     2101 ttagaaattg cctcttcacg agaaatcagc acataacgcc cggaacgaca ggcagtcaga
     2161 aactgacacc atcccggcat gactttttcc attgctgcca gctcatcctg aggtttacca
     2221 ccggtgtcac cgcgccaggt tgcaaaaaca aaatcggcat ccagttcagg taaacgctcc
     2281 gcgctcacat ccattcgccc accttcagga atgctctcaa tcagcggcgg aaaacgaaaa
     2341 ccagcatcgc gtaatacccg tcccagcgaa tgatagctat gcatcacatt aatttttccc
     2401 tggtttgcct gaatcaccga taccgttatt ttttggctgt ccagcgtcgc cttcagcgca
     2461 ttgatttgtg cctgatagcg gcgttcaagt atcgccagct gcgactgcgt tcctgtaagt
     2521 tctgccagct tgcgatagat ttctggcgcg ccccctttga gatgatcaat gctcaccgtg
     2581 ggcgcaattt tttcaagttg ttcaatgggc gtattacggg tcggttctgt gatgatcaag
     2641 tcaggctttg ccgccacaat cgcttcaata tcaatatcag cggtaccaat aaaggcgatc
     2701 gatgaattat caaagtcgac acctgtcaat aacgcgccag atcgaatgaa atggctaccg
     2761 tcaggccgag ttcgcccgtg gctggcgaca ggaggtacac cgagctcaat cagaggaata
     2821 gtgatatcaa gatcgtgtaa agagacaatt ctctttggat gagcaggaac agtgactttg
     2881 cgccctaaat catcagtaaa tacctgaaca ggttctgttg cacgggaaaa aaatgacaac
     2941 agtatcagca gagaaaaaaa taaacgcata taactcccgt taaaaactgt tcctgcgctg
     3001 ccagagcagt agtaaaaaaa atggtccacc aatcagtgag ataatgattc cggcaggcag
     3061 ttgcaaaggc aaaaaagcca aacgaccaat gctatccgcc agaataacta acaacgcccc
     3121 cgataccgcg ctccccagca gtaatgtcgt ctgcccgcca cgtagaataa aacgtgatat
     3181 atggggtgct atcaatccga caaaaccgat gttaccaaca cacgaaacgc agacggctgt
     3241 caggattatg ggtgcggcga cacgtaacaa cgccagacga gaactattca cccctaaccc
     3301 ggttgcaacc tgatgaccaa gcagagcgat atccgcagtc ctggcggtga acagcaataa
     3361 aagagcggct ggcaacatcc aacaggctga aataccgact aacatccagt tagctgcatg
     3421 caggcttccc gataaccaca tcaatgcagt ttgtacatct cgaacatctg cagtggtcat
     3481 aaatacaccg ataccggcgg cgaaaaacca ggaaacacca ataccaatca gaacaaatcg
     3541 cgggcgggaa atatcgcgcg cacaaaaaat cacgattaaa gccaccaaca ggccaccagc
     3601 aagaccagcc actggacgcc agaacattcc cagcattgga aactggaaaa tcagccacag
     3661 cacagccacg ctacatcctt ctttgacgcc gattaaacca ggatcggcaa ggccgttacg
     3721 tgcaatggat tgcattgcgg cccctgccat acctaacatg gccccgcata gcaatgccat
     3781 gatgactctc ggcaagcgaa tatcctgcac aatataacgt gcgtctgtgc ttacgttttc
     3841 tggagcaaac aacgctcgtc ctacttccga tgccggaaca gagaaagaac cctgcatcag
     3901 accgaatatc agtattccaa ccgcaagcag gaacagccca ccaagcaaca gcagattttt
     3961 agggcgcacc agtgtcgaaa acttaccaac acgtagtggg cgtagcccga cgttcattat
     4021 ccgactcatt tgaacatcct cgtcgccata atgacaaaaa caggcgcacc aactaacgcc
     4081 gtcatgacac cggtcgctaa ctcatggggc gtgaaaagcg tccgggcaat aatatctgcc
     4141 agtaataaca aaagcgcgcc gacacaagct gaaagaggga gcagaacgcg cagatccgct
     4201 gataccagac gccggacaat ctgcggtact agcaggccga caaaaccaat ggggccagca
     4261 atcgatacgg ctgcgccaca gagcaacgcg atcgcaagca gggtgaatgt tcgcgtgcgg
     4321 agtacagaga cgcccagccc ctgcgccatg cgatcgccca gtgccaacat gttaagggag
     4381 ggtgcaaggt aaagggctaa cacaaatccc cccagagaaa accaggcgga cgtacccagc
     4441 gtcgcccaat cctgccccgc taaatcacct gctagccatg tccgcatcgc cagcagtgtt
     4501 tgttcgtcaa gaatcaggat tgcagccgtt attgaggaca cgaacgccga tagcgccacg
     4561 ccgcataaag taactttcat tggcgttaac cctgaacgcc ctgcggagga aagcagcaat
     4621 atcaacagga acaataacgc gccccccgta gatgccagca gtgggcgtcc aaccggaaag
     4681 gccagtccaa gcgcactggc ggcaacgaca gcaagagcgg ctccggcatt taagcctaaa
     4741 atatgtggtt cccccaatgg gttacgaatg acagcctgca acaaagcacc agcaacacca
     4801 agagaagcac cggtaagcaa tgctgccgct aaacgcggca aacggagtct tacaattacg
     4861 ttgtgatcga agttttgcgg atcaaaatga agaaatgcct gcaacaccgt ttgtggtgca
     4921 ataaatcggg caccaatccc tggatatacc acgcatccca ccagcagtaa ggcgaggaaa
     4981 attacagtaa ccagccctgt gcgcatcata ctttttctac accacgaaac ggcatgaaga
     5041 acggctttcc cgtcagagga tttattgatg cgtgaacatc aacgtcaaag acagctttta
     5101 ccagttcagg cgtgcaatgc tccccctcat tcaacacacg caccactttc ccctggcgta
     5161 aaaatatcaa cgtatcgcca taattgacgg caaaattcag atcatgcaac accacgacga
     5221 ctgtacgccc gtgatgacgc gttaaatcat gtaataactc aagaatttca acctgataac
     5281 gtaaatcaag ccaggttgtg ggttcatcca gcaggatata aggcgtcttt tgcgccaaca
     5341 ccatcgcaat ccagcaacgc tggcgctgcc caccagagag tttttcaaca ggtaaatgtg
     5401 caaactcctg cgtaccggtt aatttcaggg cctcttccac cgcctgctca tcggcgtcgc
     5461 tccattgacg tatgaaattt tgccagggaa aacgcccacg cgacaccagt tcataaacgg
     5521 ttaagccctc cggcaataat ggcgactgcg gcaagatccc aagtctacgg gataacgctt
     5581 tcgttggttg ctcgtgaatc gctttgccat caagcaatat cgaaccgccc ataggttgca
     5641 agatacgtgc aatggtgctg agcaacgtcg actttccaca gccgtttgcc ccaaccagaa
     5701 cggtcatttt ttctgtggga acggagaaag aaacaccatc aacaatgatt ttcttgtgat
     5761 acccggcaga aacattgtcg aggatcagcc cctgcccttt tgcgttatgt gccacgtgta
     5821 cgccaactta atcaaagaca aacaaaaata aaactcattc tcctttgaga gtttgacgcc
     5881 acaaaatgta gtagtca
//
LOCUS       DACADA010000229         5715 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.232, whole genome shotgun sequence.
ACCESSION   DACADA010000229 DACADA010000000
VERSION     DACADA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.232"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ISB17_004466"
     CDS             complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ISB17_004466"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP1982326.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ISB17_004467"
     CDS             complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ISB17_004467"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP1982327.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ISB17_004468"
     CDS             complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ISB17_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP1982328.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ISB17_004469"
     CDS             complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ISB17_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP1982329.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4276..4605)
                     /locus_tag="ISB17_004470"
     CDS             complement(4276..4605)
                     /locus_tag="ISB17_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP1982330.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4757..5101
                     /locus_tag="ISB17_004471"
     CDS             4757..5101
                     /locus_tag="ISB17_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAP1982331.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5138..5587)
                     /gene="alaE"
                     /locus_tag="ISB17_004472"
     CDS             complement(5138..5587)
                     /gene="alaE"
                     /locus_tag="ISB17_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAP1982332.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1444 a         1417 c         1433 g         1421 t
ORIGIN      
        1 tctaccctaa catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt
       61 gatgtgagca aatcgccgga atttcccatg atataagggc tgagagcaaa tcgagaaaag
      121 ttgatatttt ttgtcaaaga tagcaaaaaa acgcctgaat attgtggttt caggcgaata
      181 atgcaacaga gtatcagtgt agatcaccac aaattatttg cgtgatataa agctatttgg
      241 cgggaattat ttccctgcag cgggtagtga tatttttgaa aataaccccc tcagaaattc
      301 cagtcttcat cttctgtttc gaccgctttt cccatcacat atgaagagcc tgaaccggaa
      361 aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact
      421 tccgccattt ctgcgggaaa tagcggttcg tagcccagat tcatcaacgc cttattggcg
      481 ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg
      541 gtgtattgca actcgttgtc atagagttcc agcaacaaat cgaaggcgaa actcttcaat
      601 tcttcacgtc gtgcctggga gatcttttcc aggtttttct gatatttata gccgatgtag
      661 taaccgtgga ctgcttcatc ccggataatc agacggatca ggtctgcggt attggttagc
      721 tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca
      781 agaaacacac tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata
      841 atctgtgctt ttcgctgcaa cggcgcgttt tcttcactcc aggcgtaggc ggcatcgaca
      901 tctttggtct ggcacagcgt cgagaaaatt gaactgtaag agcgggcatg aaccgcttcc
      961 ataaagctga tatttgataa taccgcctct tcatgggggg tgagtgcatc gggcatcagc
     1021 gagggcgcgc cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc
     1081 attgttagtt gttgttctac gacacttaat gtctgccatg caggaatatc gttcgacagt
     1141 ggcacctttt ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta
     1201 tcgtcagata tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct
     1261 taaagtgcac aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg
     1321 cgaatgtaat agagcgtttt gatacccttg cgccaggcgt aaatctgcgc tttgttgata
     1381 tcgcgagtgg tggcggtatc ggggaaaaac agcgtcagcg acagcccctg atcgacatgg
     1441 cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcgtcctga
     1501 tacagcgcca gattttcgtt agtcataaac ggggcagggt agtaaacgcg cccggttttg
     1561 ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt ggcatggttg
     1621 atataagaga tagaaccggt tggcggcacc gcctggagat tctggttgta gataccatag
     1681 cgcatcacgt cgtcgcgcag ctgcgcccac atctcacggg taggtaacgt aataccgcta
     1741 cgggcaaaca gttcgccaac tttcgccgtt ttcggctgcc agttactttg cagatattgg
     1801 ctgaaatatt caccactggc atagcgtgac tgtttgaacc cggcgaaggt ttcaccgcgt
     1861 tcgcgcgcca gcaacatcga ggtacgcagt gcgtgccaag tgatggtata gaaatagaga
     1921 ttggtgaaat cgagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg
     1981 tgtaaattca tctgccccag tccgatggcg tgcgaggcgg catttccggc ttcgatggac
     2041 ggcacgctgc ggatatgact catatctgac accgccgtta aaccgcgtac ggcagtctct
     2101 accgtgcggg caaagtcggg ggaatccatg gtgtgggcaa tattcaacga ccctagattg
     2161 caggaaatat catggcctgt acgggcatag tcgagattct cgtcatattc tgaggcgctg
     2221 ttaacctgca aaatttctga gcagagattg ctcatattta tgcgcccggc gatagggtta
     2281 gcacggttaa ccgtgtcttc atacatgatg tacggatagc cagactcaaa ctggatctct
     2341 gccagtcgct ggaagaaatc acgagcgttg aggtattttt tgcgaatgcg ttcatcggca
     2401 accagttcgt catagcgttc gctgatggcg acatcggcaa acggtttgcc ataaacccgc
     2461 tcaacgtcat agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg
     2521 atatccggga tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt
     2581 tcccgttttg tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgcc
     2641 ccagccccct gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc
     2701 atcaccggaa tcacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttcc
     2761 cgcagattcg acagcaaaaa tgctacgccg ccgccgcgtt tcgacagttg tagtgcggaa
     2821 tttactgccc ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc
     2881 agttcgccgc gctgctgctt accgcagttg aggaatgttg gcgtggctgg ctgaaagcgt
     2941 cctgacagca tctcatcggt gagctgcgtc gccagtgttt catcgccttg tgccagcgtc
     3001 agcgccacca tcgtgacgcg atcggcaaaa tcttccagat aacgcttacc gtcgaatgtc
     3061 ttcaacgtat agctggtgta aaacttccat gctcctagga atgtctggaa acgaaaaccg
     3121 ctggcgtgcg cgtgggcaaa cagcgtaatg acaaaatcgc gagagtagcg attaaggaca
     3181 ctttcatcat agtaaccttc gttaaccagc cagttcaggc gctgctgctg gctactgaag
     3241 gccacactgt tcggacgcac atgcgtcgtc ataaaggcgt ctactgcctg gcgatcttta
     3301 tcgaactgaa tgcgacctgc gctatcgtaa aggttaagca tcgcattcag agcgtgataa
     3361 tccatcgttt cctgcgtcag gcgttctgcg gttgtcgttg ccaaaattca gttactcctt
     3421 tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaagcggtac agccacggca
     3481 cgccgcattt ccgggcaatc acatctccgg cgcggccata tgcctcacca aagttgcgat
     3541 tacccgaagc aataacgccg cgcagcaacg cccggttatg ctcgtcgttt aaaaagcgaa
     3601 tgacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga
     3661 tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca
     3721 gccctaaacg ttcgataaaa cgctgcgtat tttcggagct gctggaaaag tagacgagct
     3781 ggctcatgca ctggccgcgt gtggcgctgg atgcagacga ttgatcatgt ccgggcggaa
     3841 accagaccag ctaagatcgc ctgcaatcac aaccggcaac tgacgaaagc cctgagcacg
     3901 cagcgcttct gccgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg
     3961 gttttccatc gcccgtttag tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt
     4021 aatgcgcatg attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg
     4081 ttgtctctct gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat
     4141 atatagcaaa taatggtcag ggttaccctg gggcgagcgt gacggggtag ggggattttt
     4201 gtgattcacc aggcaaaaag aaaccccgaa gccaggcttc ggggtcaaag acgcgtattt
     4261 attatcattt ttgcactacg atttgcgcat gcttaacagt gcgccgataa aaatacccac
     4321 cgctgccgct gtacccacac tacaccaggg tctttcacga acaaaagaat ccgcgcagcc
     4381 aacggcatcg cgcgcggctt gctggacgcg agtacgacca tgcattcgcg ccctggtttc
     4441 tttcagtaga gcctgcgctt tactacgagc ggcttcggct tcccctttgg cgtcgctgcc
     4501 ccaggacttc aatacagatt cgagactgtc agctaattga ttgacatcat tctggatatc
     4561 ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta aacatatgat cccccattag
     4621 aattcgatgt aattttcagt gtagaccata attttcttaa ctgaggttca tcgcggcttt
     4681 atggactttt ctgaaagcgt tgtgaatgcg caatcaggta aggtaggccc gcggttgata
     4741 ataacgagga tcaaatatgt atttacgacc agacgaggtg gcgcgcgtac ttgaaaaagt
     4801 cggttttact gtcgatgtgg taacccagaa agcgtatggt taccgccgtg gggaaaatta
     4861 tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgcc ctggtgattc atccaacatt
     4921 aaaagaacgt agttcaacac tggctgaacc cgcttccgat attaaaactt gcgatcatta
     4981 ccagcaattt ccgctctatt tagcgggtga acgtcatgag cattacggta tcccgcatgg
     5041 ctttagttcg cgtgttgcgc ttgaacgtta tttgaatggt ttatttggcg aagccagtta
     5101 aagacgcgac tggcgatgcc agtcgcgaaa agaagggtca ggcttttacc tgctggtaac
     5161 ggctgacttt aaacagtcgg cggcaataat cgaggaagta gccataaacc gcccccatca
     5221 acatcgaaac aacgatgttt gaactgactg ccgccataat ctgatgccag tctgcgccca
     5281 ccactaacaa gatcgccaca tacaccggtg actggaacgt cacataagcc aggatatccg
     5341 ccagattttt tatccagcct gacgggctaa ctttgcgtgc cgcgcgcata aacagatcgc
     5401 ggtacatacc gtatggccat gcaattaaga tgttcaccgg aatcgctacc aatctggaat
     5461 aaaaagactg ttcgaagctc attccggaga ggaaaacttc aatacacatg ttcacgacag
     5521 aacagtaaac aaccatcgcg aacgtatctg caactgcatg acgcaagcgt gactgcggtg
     5581 agaacatgct aatgctcctt aataaaagcg taatgggatg ttaatggaga tgaaagcagt
     5641 ggatctcatt gccatctgca tttttgcata gcgtatatcg ctggctatga ctaatcaatt
     5701 agtgaaaaat tttca
//
LOCUS       DACADA010000230         5652 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.233, whole genome shotgun sequence.
ACCESSION   DACADA010000230 DACADA010000000
VERSION     DACADA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.233"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            466..1368
                     /locus_tag="ISB17_004473"
     CDS             466..1368
                     /locus_tag="ISB17_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAP1982333.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            1372..1676
                     /locus_tag="ISB17_004474"
                     /pseudo
     CDS             1372..1676
                     /locus_tag="ISB17_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1753..2436
                     /locus_tag="ISB17_004475"
     CDS             1753..2436
                     /locus_tag="ISB17_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1982334.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            2437..2658
                     /locus_tag="ISB17_004476"
     CDS             2437..2658
                     /locus_tag="ISB17_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982335.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2672..3106
                     /locus_tag="ISB17_004477"
     CDS             2672..3106
                     /locus_tag="ISB17_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1982336.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            3157..3921
                     /locus_tag="ISB17_004478"
                     /pseudo
     CDS             3157..3921
                     /locus_tag="ISB17_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3806..4377
                     /locus_tag="ISB17_004479"
                     /pseudo
     CDS             3806..4377
                     /locus_tag="ISB17_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            4473..4775
                     /locus_tag="ISB17_004480"
     CDS             4473..4775
                     /locus_tag="ISB17_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1982337.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            4822..5244
                     /locus_tag="ISB17_004481"
     CDS             4822..5244
                     /locus_tag="ISB17_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1982338.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            5241..5432
                     /locus_tag="ISB17_004482"
     CDS             5241..5432
                     /locus_tag="ISB17_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982339.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT         1152 a         1535 c         1727 g         1238 t
ORIGIN      
        1 tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg
       61 atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattgaca
      121 tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat
      181 tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg
      241 gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc
      301 gccggcctcc tcacccggtc acgtttcgtc gttcctcctc cacgcgctgc ggcttcgggg
      361 ccgcacctgc attcgtatgc ggtcgcccgg ttacaggtgc ggcacggcct gatggaggcc
      421 gcatgtgaga ggagaattcc catgccaaac tggtgctcga atcgtatgca tttttctggt
      481 gaaccggcac agattgctga gattaaacga ctggccagcg gtgccgtcac accgctttat
      541 cgccgcgcca caaatgaagg tattcagctg tttctggccg gaagtgccgg acttctgcag
      601 accactgaag atgtgcagtt tgaaccgtgc cccggactga cggatgccgg acgtggtgtt
      661 gtatcgccgg agaatatcgc gttcacccgc tggctgacac acctgcagaa cggtgtactg
      721 ctggatgagc aaaactgcct gatgctgcat gaactctggc tgcagagtgg tactggccag
      781 cgtcgctggg aaggattacc ggatgaggtc agggaaacca tcaccgtgca tttcaccgca
      841 aaaagaggtg actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt
      901 ctgtgtgaca atgtactgcc ggaaaaaacc atgccgtttg acctgctgac ggttctgccc
      961 acccgcctgg atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct
     1021 gcgtatcact ggtatacgga acagtatggc gtgaagtggc ctgtggggta tgaggtgaat
     1081 atcagccgtc agggagaaaa cttcattcaa gtggatttcg acacgccgtg gtgtcagccg
     1141 gaaagcaacg ttgttgcaga attaagccgc cgtttcggct gcacgctgga gcactggtac
     1201 gccgaacagg gctgcaattt ctgtggctgg cagcgatatg agcgtggaga gcttgttgat
     1261 gtgctgtggg gggaactgga atggtcttcc ccgacagatg atgatgagct gccggaagtc
     1321 accgcgccgg agtggatagt cgacaaggtg gcgcattacg ggggatgaag tatgacggag
     1381 acgggcgggc aaccgcccgt ttcttttccg acaaaggatg tcgccgtgct cctcttttta
     1441 ctgcgccggc tgacgcggcg cggcaggaac gctgcctgtg gtcagtgtcc cgcgtccggt
     1501 gggcacggga cgggcgcttt tactgctccc ggctggcacc ggtgacagtg tacgccagcc
     1561 tgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatattc tcgccgtcaa
     1621 aaagacgacg gctgcgatat tccacggtcg gcctgacccg ttaccggacg cggtgaacag
     1681 cccacaggca gcggggaacg ggcaccacag gggggccctc ccggtgccct ctgtaagaga
     1741 aggagcgttg ttatgtcccg ttttgtcctt ggtaactgca tcgatgttat gacgcgtatc
     1801 cctgataacg ccattgattt catattaact gacccgccat atctcgtcgg ttttcgtgac
     1861 cgttccgggc gcaccatcgc cggcgataaa accgatgaat ggctgcaacc ggcctgtaat
     1921 gaaatgtacc gcgtactcaa aaaagacgcg ctgatggtga gcttctacgg ctggaaccgc
     1981 gtcgatcgct ttatggccgc ctggaaaaat gcgggattca gcgttgttgg tcacctggtc
     2041 ttcaccaaaa actacacatc gaaggccgca tatgtgggct atcgccacga atgcgcctac
     2101 atcctggcaa aaggccgtcc acgtctgcca caaaacccgc tgccggacgt gctgggctgg
     2161 aaatattctg gcaatcgtca tcacccgacg gaaaagcccg ttaccagcct gcaaccgctg
     2221 attgagagct tcacacaccc gaacgcaatt gtgctggacc cgtttgcagg cagcggctca
     2281 acctgcgtcg ccgccctcca gtccggacgc cggtatatcg gtatcgagct gcttgagcag
     2341 tatcaccgtg ccgggcagca acgccttgcc gccgtgcaac gggccatgca gcagggggcc
     2401 gcgaatgatg actggtttat gccggaggct gcgtaaatga actatgccgg acacgaaaaa
     2461 cttcgcgccg acgtggcgga ggtggccaac accatgtgtg accttcgggc caggctgaac
     2521 gatatggagc atcgctgtcg ctttgattcc gatgtgcttg ttgaacgcct gacacgccag
     2581 acgctgtatc gcgccaatcg cctgtttatg gaggcgtata ccgaaattct ggaactggat
     2641 tcctgcttca aagattgaga aggagacgag aatgtacgga acatgcgaaa cgctatgccg
     2701 ggagctggca gcaaagtatc cgggagacgc gccgctgatg ctggttatct ggtccccgga
     2761 agagattcag gcactggctg acggtatgga aatttctctg accgatcatg aaatcaggac
     2821 cgtcctggcg cgcatggagg acatcccgga agaccagcgg actgaatccg gtatttcttc
     2881 cgccgcggtg atggagatta tcagcaacgt gagcgaaaac cgccaggtga ccgtccctgc
     2941 tgaactgctg gcgtccctga ttcagaccgc tgaacaggca ctgtggaaac gtgagtgggc
     3001 cgcccgtgat aacggcctcg ccgtcccgga atgcgttacc cgccgtcagg cggtggttaa
     3061 tcaggcccgc accctgctga aaaacaacac acacgaaaac aactgatgtt atcgccgcct
     3121 gccctgcggg cggcgataca gggagaacgc ttaatcatga acgaaaacag cacgctgaac
     3181 gcactgattt gtcgtcacgc ccgtaacctg ctgctggcgc agggctggcc ggaagagacg
     3241 gatgttgacc agcgaaatcc gaactatccg ggctggatca gcatttacgt gcggctggat
     3301 gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc gccgctcctg
     3361 gcctccgcca ttcagagact gaccggaacc ggggcggaac tggtactgtc cggcagtcag
     3421 tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccgga
     3481 gaatggctga ccgaagacga aatcagggct gttcttgatg cggtgcacga tgcggtacga
     3541 agcatctgtt accaggtggc agaagatgcg cggcgtatcc gtgcggcgct gaccaccacc
     3601 ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggtcgtgaa ggaaagcgat
     3661 cacccctgct ggctcgatga agatgacgaa aacctgcccg tggtgctcga tgccatcgtg
     3721 aaccggggag cacgtttttc gtcggtggaa atgtatctgg tcagtgagtg tattgagcat
     3781 atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac cggatgaacc gccccgccgc
     3841 tggtttgacc gtggtgttct gcgggaggtg gtccgggaag cccggaccga aatccgcagc
     3901 atggcggatg ccctggcaaa aatccggaaa tgatgacact ggcggggtaa atccccgcct
     3961 ttttcctgcc ggtccccgga cagagcacaa aggaagtcgc cgccggctcc gctttacccg
     4021 gccatgcagg gcatggcctt gtggtttttc agttatgtgg cctcagcgtc gtgtgcgggc
     4081 tgtgccgtgc ctccatctta gccgggctgg cgttgatgca agggtacgct tcgccgctgc
     4141 ggtcacccgg tccctccttc ccgtctgccg tgattttccg gtcgttcacc cgctcagatt
     4201 tcgcggtctc accctgcaac tcccgtcagc cgtgtgcgtc aggctgcggc ttcccttgcg
     4261 cccctgcatc cccgcctgtt cgcccggctt ttatggaggc acggcaccgc ccggtgtcgc
     4321 ggaatatgtg aacaacggag gaaaacatca tgcaatatgc gaaacctgtc actctgaatg
     4381 ttgaagagtg cgaccgtctc tcctttctgc cgtatctgtt tggcaacgat tttctgtatg
     4441 ccgaggcgta tgtgtacgcg ctggcgcaaa aaatgatgcc ggaatatcag ggaggcttct
     4501 ggcacttcat ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgtttcc
     4561 acatggtgaa cggtgcaaac tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca
     4621 tcctgacctc ccttgtgatt aaccgccagt tgtggctgta ccacgacagc ggggatgcag
     4681 gactgaccca gctttaccgg atgcgcgatg cgcagttgtg gcgtcacatc gaatttcacc
     4741 ccgaatgcaa cgcgatttac gcggctctgg actgattaac ggacggggcg gcaatgccgc
     4801 ccctgtaaag gagacgagaa catgtactgt actgttaaag aaattatccg cgaagtgctg
     4861 gatacagacg tgccggacag tgaatgcgtt tttgccgtag tgctgacccg tggggatgtg
     4921 cgtcacatag cccaggactg gagtctgaca gacgatgagc tggaaaccgt catgcagcgg
     4981 ctggacgatg cctttgaata tggtgcggat gtcagcgttg ttcacggcgt tgttcgtgaa
     5041 ctgatggaag aaaagcgcgc cagccgtcag gtgacagtcc cggcggtgat gctggaaaaa
     5101 gtgatggcgc tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg
     5161 ggcgacggtg acgcattcgt cagggaggaa cgcgaagcaa tggacgttgt gttacaggcg
     5221 ctggacgggg agacgatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgtaatt
     5281 acagggccgt gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc accaccgcgc
     5341 agattgttga tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctgggcggtc
     5401 actacatctt acagggtagc agaaacaggt gatacagggg caggcggtgg aggccgcctg
     5461 tcagtccgcg cgaaaacccc tggcaggctg acgcctgccg gaagggaagc gggccacgct
     5521 ggcgcgtcgc ccgttaccct tcccgtctgt gatgtgtctg actcaggagt gtttctttct
     5581 tatgttatgg aactgttcct gatgttgttg ttcctttatc tgctgctctg attctgacta
     5641 ctgcagtgtt gt
//
LOCUS       DACADA010000231         5578 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.234, whole genome shotgun sequence.
ACCESSION   DACADA010000231 DACADA010000000
VERSION     DACADA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.234"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(119..655)
                     /gene="ssb1"
                     /locus_tag="ISB17_004483"
     CDS             complement(119..655)
                     /gene="ssb1"
                     /locus_tag="ISB17_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAP1982340.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            910..3732
                     /gene="uvrA"
                     /locus_tag="ISB17_004484"
     CDS             910..3732
                     /gene="uvrA"
                     /locus_tag="ISB17_004484"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP1982341.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPDGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3767..4123)
                     /locus_tag="ISB17_004485"
     CDS             complement(3767..4123)
                     /locus_tag="ISB17_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP1982342.1"
                     /translation="MTILELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4127..4543)
                     /locus_tag="ISB17_004486"
     CDS             complement(4127..4543)
                     /locus_tag="ISB17_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP1982343.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4654..5367)
                     /gene="aphA"
                     /locus_tag="ISB17_004487"
     CDS             complement(4654..5367)
                     /gene="aphA"
                     /locus_tag="ISB17_004487"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HAP1982344.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1337 a         1463 c         1449 g         1329 t
ORIGIN      
        1 aagccctcta gtatctttac agacaaaaga gggctattta ttaacttaac ctattaattt
       61 atatgtcttt tttaaaacaa accaaaccta aaagattaca aattaatatt tatcagggct
      121 agaacggaat atcgtcatca aagtccatcg gcggctcgtt agacggcgct gccggagcgg
      181 actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccaccctgc ggctgctgag
      241 gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgca ggagcgccac
      301 caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg acttctgtgg
      361 tatagcgatc ctgaccggat tgatcggtcc atttgcgggt acgcagctga ccttcgatat
      421 aaacctgaga acctttacgc agatattcgc tggctacttc tgccagtttg ccgaacagca
      481 caacgcggtg ccattcagtc tgctctttca tctcgccggt ggctttatca cgccaggatt
      541 cggaagtagc cagcgtaatg ttggcaactg cgccaccatt tggcatgtag cgtacttccg
      601 ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg
      661 tgtctcctga aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac cattaccaat
      721 agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag acattgttac
      781 acaacactcc gggtaatgca ttccaatact gtatattcat tcaggttaat ttgtgtcata
      841 attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg tttaatccgg
      901 gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct caaaaacatc
      961 aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg ttctggcaaa
     1021 tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt tgaatccctt
     1081 tccgcctacg cgcggcagtt tctgtcactg atggagaagc cggacgtcga ccatattgag
     1141 gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc gcgctctacg
     1201 gtggggacaa tcaccgaaat ccacgactat ttgcgtctgc tgtacgctcg cgttggtgag
     1261 ccgcgctgcc cggaccacga tgtaccgctg gcggcgcaaa ccgtcagtca gatggtggat
     1321 aacgtgttgt cccagccgga cggcaagcgt ctgatgctgc tcgcgccaat cattaaagag
     1381 cgcaaaggcg aacacaccaa aacgctggag aacctggcaa gccagggtta catccgtgct
     1441 cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact gcaaaagaaa
     1501 cataccattg aagtggtggt tgatcgcttc aaagtgcgtg acgatcttac ccaacgtctt
     1561 gccgagtcgt ttgaaaccgc gctggagctt tccggtggta ccgcggtagt ggcggatatg
     1621 gacgacccga aagcggaaga gctgctgttc tccgccaact ttgcctgccc gatttgcggt
     1681 tacagtatgc gcgagctgga acctcgcctg ttttcattta acaacccggc gggagcctgc
     1741 ccgacctgtg acggacttgg cgtacagcaa tatttcgatc ctgaccgcgt gatccagaat
     1801 ccggaattat cgctggctgg cggtgcgatc cgtggctggg atcgccgcaa cttctattat
     1861 ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc gccgtggggc
     1921 agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga aaatattgaa
     1981 ttcaaataca tgaacgatcg tggcgatacc tccattcgtc gtcatccgtt cgaaggcgtg
     2041 ctgcacaata tggagcgccg ttataaagag acggaatcca gcgcggtacg tgaagaatta
     2101 gccaagttta tcagcaatcg tccgtgcgcc agctgcgaag gaacccgtct gcgtcgggaa
     2161 gcacgccacg tgtatgtcga gaatacgccg ctgcccgcca tctccgacat gagcatcggt
     2221 catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc agcgggcgaa gattgcagaa
     2281 aaaatcctta aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg cctgaattac
     2341 ctgacacttt cccgctcagc agagacactt tccggcggtg aagcccagcg tattcgtctg
     2401 gcgagccaga ttggtgcagg cctggttggc gtgatgtacg tgctggatga gccgtctatc
     2461 ggcctgcacc agcgcgataa cgagcgcctg ctgggtacgc ttatccatct gcgcgatctc
     2521 ggtaataccg tgattgtggt ggagcacgac gaagacgcga ttcgcgccgc tgatcatgtg
     2581 atcgatatcg gtccgggtgc gggtgttcac ggcggtgaag tcgtcgcgga aggtccgctg
     2641 gaagcaataa tggcggtgcc tgagtcgttg accgggcagt acatgagcgg taaacgcaag
     2701 attgaggtgc cgaagaaacg cgttccggca aatccggaaa aagtgctgaa actgacgggc
     2761 gcacgcggca acaacctgaa agacgtgacg ctgacgctgc cggtgggtct gtttacctgc
     2821 attaccggag tttcaggttc cggtaaatca acgctgatta acgacacact gttcccaatt
     2881 gcccaacgcc agttgaatgg tgcgaccatc gccgaaccag caccgtatcg cgatattcag
     2941 gggctggagc atttcgataa agtgatcgat atcgaccaaa gcccaattgg tcgtactccg
     3001 cgttctaacc cggcgaccta taccggcgtg tttacgcctg tgcgcgaact gtttgcgggc
     3061 gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt tcagctttaa cgttcgcggc
     3121 ggacgctgcg aagcctgtca gggcgacggc gtgatcaaag tggagatgca cttcctgccg
     3181 gacatttacg taccgtgcga ccagtgcaaa ggcaaacgct ataaccgtga aacgctggag
     3241 attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga agaggcgcgt
     3301 gagttctttg atgcggtgcc agctctggca cgtaagctgc aaacattgat ggacgttggt
     3361 ctgacgtata ttcgtctggg tcagtccgca accacacttt ctggtggtga agcccagcgc
     3421 gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta tattctcgac
     3481 gagccgacca ccggtctgca cttcgccgat attcagcaac tgctcgacgt actgcataaa
     3541 ctgcgcgatc agggcaatac cattgtggta attgagcaca atctcgacgt gatcaaaacc
     3601 gctgactgga ttgtcgacct gggaccagaa ggcggcagtg ggggcggcga aatcctcgtc
     3661 tccggtacgc cagaaaccgt cgcagagtgc gaagcttcgc atacggcgcg cttcctcaag
     3721 ccgatgctgt aatcgttaag gccgctttct gagcggcctt cttctttcag agttgcacca
     3781 gcaatttacg tttttcttcc ggcagcaaat tcaccgcctg ctgataagac gcatccacca
     3841 gataatagat ttgcgaatcc ggcagcgatc cgtcgagata cacggtgctc cagtgtgctt
     3901 tgttcagatg gcggcttgga cgcacatcgc tgtgctgctg acgtagcagc tccgccagct
     3961 ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc atcgcaaaca
     4021 gcacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc tgttctgcgc
     4081 ctggttttgc catgcaatat tgtagcaact ccaaaatggt cattttttac tccccttgaa
     4141 gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc cagatgcctt
     4201 gccaggtgcc ggtctgaata cgccctttat gcaccggcaa tacaagcgat gttcccagca
     4261 ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc tcataatttc
     4321 cgttgtcggg aacggcgcgg aggaaaaaac gctccatgtc gtggcgtacg gtgggatcgc
     4381 agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc agtaaaccga
     4441 tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc agatgaaacc
     4501 cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc tccttgataa
     4561 aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat taaaaaaaca
     4621 gcattcggga gagtattacg ctctcccgct ctgtcagtat tctgaattga cgatcacctc
     4681 ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg cgcgcagaat
     4741 gcggatacca cgagcgccaa catcgcgtgc ggcggtaata tcgttatcgg aatcaccata
     4801 aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgac ctggtttatc
     4861 gcccgcaaag atcaccggat tcatgttggt ggcaggaata tgaaaattat ccgccagcgt
     4921 ttttgaaacc gtttctgttt tggtcgggct acgaccagtc acaaagaaga tcgcgtcacc
     4981 gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc tgaattcatc
     5041 ccagccattg ttcatttttt cccagaacac aggatttttc agataatctt cgctttctgg
     5101 tgagaaggtt tttttgccgc gccagaaacc cggactggaa aaaagtaccg tatcatcgat
     5161 atcaaacccc accgccattg gcggacgtcc tgcgaggcta ttttcaattt gtgcaaccga
     5221 aacccaatga attggtgcct gttcagcgag tttggctacg ttagtacccg gattaagcgg
     5281 tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata agcaaacggc
     5341 actcaatgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtaa gttgaaactt
     5401 attattgtgc gtttgttgaa aaaaccatct gaccataacg ccaaccgcag gggaaaggaa
     5461 ggattatctg cggatttgtc acataaaaag agcatgatca catttggaat acctgtgcga
     5521 taaaccacac attgccacgg ttattctgct tacacattca cacaatggca ctcttttg
//
LOCUS       DACADA010000232         5563 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.235, whole genome shotgun sequence.
ACCESSION   DACADA010000232 DACADA010000000
VERSION     DACADA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.235"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(31..990)
                     /gene="rfaC"
                     /locus_tag="ISB17_004488"
     CDS             complement(31..990)
                     /gene="rfaC"
                     /locus_tag="ISB17_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAP1982345.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
     gene            complement(994..2040)
                     /gene="rfaF"
                     /locus_tag="ISB17_004489"
     CDS             complement(994..2040)
                     /gene="rfaF"
                     /locus_tag="ISB17_004489"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAP1982346.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2050..2982)
                     /gene="rfaD"
                     /locus_tag="ISB17_004490"
     CDS             complement(2050..2982)
                     /gene="rfaD"
                     /locus_tag="ISB17_004490"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAP1982347.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3196..4392
                     /gene="kbl"
                     /locus_tag="ISB17_004491"
     CDS             3196..4392
                     /gene="kbl"
                     /locus_tag="ISB17_004491"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP1982348.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4402..5427
                     /gene="tdh"
                     /locus_tag="ISB17_004492"
     CDS             4402..5427
                     /gene="tdh"
                     /locus_tag="ISB17_004492"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP1982349.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT         1275 a         1409 c         1463 g         1416 t
ORIGIN      
        1 aagtttaaag gatgttagca tgttttacct ttataatgat gataactttt ccaaaactgc
       61 ttgactgttg aggttaatta aattttctct tggagcccta caaaccatct gattcttccc
      121 atacccacca attaatcccg gatcggtagg tccataaacc gtgatattgg gtctatccag
      181 tgccgccgtt aaatggctta accccgtatc caccgacact acaaatttag ccccggccag
      241 tacgcgggca acgccttcca gactcatctt cggcaatact tcaacataag caaatccttc
      301 cgccagtcgt ttcgcccgtt cttcctcatg cggcgcgccc cacggaagtt taatccgtat
      361 tcctgaatca gccagtaaac caatcaattc tcgccagtgt tcttccggcc agtgtttatc
      421 atctcgggtt gttgcatgaa gaaatacggc atattcgcca gcatctgtag gcaggttcgt
      481 caggaaatgc tgtgcgatag cataatcgcc ctgggtttgt ggtttgctat agcccagact
      541 tttggcaaac agttcgcggg tgcgttctac ggcgtgctgc tgttttgcaa tatgatgttt
      601 acgattgtaa aacaggctgg ctaacggttc gcgagcggtt tgccagtcca ttccatgctt
      661 tacgccatgc gccagacgcg tcaccagcgt cgcgcttttt accagcccct gagcgtcgat
      721 aactgcgtca tagttctctg cttgtagcgc ttcacgaaac gctttgcgtt cagcttttat
      781 gggggccgag aaccaggctt tacgccagcg acgtattgcc acaggaataa ctcgctcaac
      841 ggcagcgtgc caggaaggaa tctgtgcgaa cccttcttcc accacccagt caaacttaat
      901 ccctgggatt gcctgctggg catcagtgag tgcgggcagc gtatggagaa catcgcccat
      961 cgacgatgtt ttaacgatca gaacccgcat ccgtcaggct tcctcttgta acaatagcgc
     1021 gttgagttct tccagtacgc gctggggagt aatgtcgatc aagctctggt gataaccctc
     1081 cgccgcgtca cctttacgca ctttgtgata gccggtaatc aggcggatca cgcgtgcttt
     1141 atgggatagc ggtggtgtga agtccgggct actcggacca tacagggcaa ccagcggacg
     1201 attgagcgcc gccgcaacgt gcatcaggcc agaatcgtta gtaacaatgg ctttacaggc
     1261 tgcaatcagg ataaccgctt gatcaagctg tgtttccccc gccaggttcc gacaccatgc
     1321 ctgctgctcg gtattcaaag cggcaagaat ctcattgccc gcttcatgat ctttcgccga
     1381 gccaaacaga accacctgat aaccttcatc aatcagctgc tttgccagct ccgcatagtg
     1441 gtagtgtggc cagcgttttg ccggaccaaa ctccgcaccc gggcaaaaac caatcatcgg
     1501 acgttctgat gagagcgaaa attgattaca ggtatatgat ttttcacctt cgctcacctg
     1561 caactgcggc cataacaatg gctgcggcag atcttgtgct gtacgcataa tgcctttgtc
     1621 ataggccagc gcgacatagc gttccaccat tagcggccag gcttctttat cgagcacgcg
     1681 tacatcgttg agtaaaccgt agcgcatctc gccgcgccag ccggtgcgat gaggaatacc
     1741 tgcgaagaaa ggcactaatg cggatttgaa ggagttaggt aagacgtagg cgcggtcgta
     1801 gcgcttttca cgcaggctat gacccagttt gcggcgttcg ccgatttcca gcgctccgtg
     1861 accgagaggc atagggatag cttcgttaac ttccggcatc cgcgataata atggacggca
     1921 ccatgccggt gccatcacgt cgattatcgc ctggggatag cgcgcctgga gcgtgcgata
     1981 gagactttgc gacatcatca tgtcgccaac ccaagacggg ccgatcacca gtattttcat
     2041 gcagagctct tatgcgtcac gattcagcca ggccatgtat tccgttacgc cttcggcaac
     2101 ggttttgaac ggtttatcgt aacccgccgc gcgcagattc gtcagatctg cctgagtgaa
     2161 cgcctggtag cggcctttca gtttatccgg gaacggaatg tattcgattt ggcctttctt
     2221 gtgataagca agcgtagcat ctgctactgc ctggaaggat tccgcacgac cggtgccgag
     2281 gttgaagatg ccggaaacgc cattttccag gaaccacagg tttacatctg ccacgtcgcc
     2341 cacatagacg aagtcacgtt taaagttctc gctaccttcg aacagcttcg gtgattcacc
     2401 gttattaagc tgggtgttga gatggaaagc gacgctcgcc atactgcctt tatggccttc
     2461 acgaggtcca taaacgttga aataacggaa gccaacaatc tgcgagttcg cttcaggcag
     2521 gatttggcga acatattcat caaacaggaa ttttgagtaa ccgtagacat tcaacggttt
     2581 ttcatattcg cgggattcaa taaagtcgga ggtgcgtcca ccgtaagtgg ctgcggaaga
     2641 ggcatacagg aacgggattt cgcgctccag acagtagtgc agcagctctt tggagtattg
     2701 atagttgtta tccatcatat acttgccgtc ccactcggtg gtggaagagc acgcgccttc
     2761 gtggaaaatc gcttcgacat cgccgaactc ttcgccagcc ataatctgga tcaggaagtc
     2821 ttccttatcc atatagtccg cgatatccag atccaccagg ttcacaaact tggtgccgtc
     2881 tttcaggttg tccaccacca gaatatcggt gatgccttta tcattcaggg ccttaacgat
     2941 gttgctgccg ataaagcccg cgccgccggt aacgatgatc ataactgtaa ccttcgaatt
     3001 atggagtcag agacaatctc agacacgaat acttctatca tatcactaca cggcgttggc
     3061 ttcagccatt cgccaaaagt gagattgatg gacgttattt atcctgaatt ttgcagaagt
     3121 gttaacgcgt catctcgtcg cgacctagaa gtttgggtaa tatgtgctgg aatttgccct
     3181 gtctggagaa tcgcaatgcg tggagaattt tatcagcagt taaccaacga tctggaaacc
     3241 gcacgggcgg aagggttgtt taaagaagag cgcattatta cgtctgcgca gcaagcagat
     3301 atcactgtgg ctgatggaag ccacgttatt aacttttgcg ccaataacta tctcggactg
     3361 gcaaatcatc cagagctgat tgcggcggca aaagcgggaa tggactctca tggttttggg
     3421 atggcttcgg tgcgctttat ctgcggcacc caggatagcc ataaagagct tgaacaaaaa
     3481 ctggcggcat tcctggggat ggaagatgcg attctctact cttcctgctt tgatgctaac
     3541 ggcggcctgt ttgaaacgct tctgagcgcg gaggacgcca ttatctccga cgcactgaac
     3601 cacgcgtcta ttattgatgg tgtgcgtctg tgtaaagcta aacgctatcg ttatgccaac
     3661 aacgatatgc atgagctgga agctcgtctg aaagaagcgc gtgaagccgg tgcgcgtcat
     3721 gtgctgattg ccaccgatgg tgtgttctcg atggacggcg tgattgccaa cctgaagggc
     3781 gtttgcgatc tggcagataa atacgatgcc ctggtgatgg tagacgactc ccacgcggtc
     3841 ggttttgtcg gtgaaaatgg tcgtggttcc catgaatact gcgatgtgat ggggcgggtc
     3901 gatattatca ccggtacgct tggtaaagcg ttgggcgggg cttctggtgg ttacaccgca
     3961 gcgcgtaaag aagtggttga gtggctgcgt cagcgttctc gtccttacct gttctccaac
     4021 tcactggcac ctgccattgt ggctgcttcc attaaagtgc tggagatggt cgaagcgggc
     4081 agtgagcttc gtgaccgtct gtgggcgaac gcgcgtcagt tccgtgagca gatgtcggcg
     4141 gcgggcttta ccctggcggg tgccgatcac gccattattc cggtcatgct tggcgatgct
     4201 gtagtggcgc aaaaatttgc ccgtgaactg caaaaagagg gcatttacgt caccggtttc
     4261 ttctatccgg tcgttcctaa aggtcaggcg cgtattcgta cccagatgtc tgcggcgcat
     4321 acccctgagc aaattacgcg tgcagtagaa gcattcacgc gtattggtaa acaactgggc
     4381 gttatcgcct gagggtgtga gatgaaagcg ttatccaaac tgaaagcgga agagggcatc
     4441 tggatgaccg acgttcctgt accggaactc gggcataacg atctgctgat taaaatccgt
     4501 aaaacagcca tctgcgggac tgacgttcac atctataact gggatgagtg gtcgcaaaaa
     4561 accatcccgg tgccgatggt cgtgggccat gaatatgtcg gtgaagtggt aggtattggt
     4621 caggaagtga aaggcttcaa gattggcgat cgcgtttctg gcgaaggcca tatcacctgc
     4681 ggtcactgtc gcaactgtcg tggcggtcgt acccatctgt gccgcaacac gatcggcgtc
     4741 ggtgttaacc gtccgggctg cttcgccgag tatctggtga ttccggcgtt caacgccttc
     4801 aaaatcccag acaatatctc cgacgacctg gcttccattt ttgatccctt cggtaacgcc
     4861 gtgcataccg cgctgtcgtt cgatctggtt ggcgaggatg tgctggtctc tggtgcaggt
     4921 ccgataggta ttatggctgc ggcggtggcg aaacacgttg gtgcacgcaa tgtggtgatc
     4981 actgatgtta acgaataccg ccttgagctg gcacgcaaaa tgggtatcac ccgtgcggtt
     5041 aacgtcgcta aagaaaacct taatgatgtg atggcagaac tgggcatgac cgaaggcttt
     5101 gatgtcggtc tggaaatgtc cggtgcgccg ccagcgtttc gtaccatgct cgacaccatg
     5161 aaccacggcg gtcgtattgc gatgctgggt attccaccgt ccgatatgtc tatcgactgg
     5221 accaaagtaa tctttaaagg cttgttcatt aaaggtattt acggtcgtga gatgtttgaa
     5281 acctggtaca agatggcggc gctgattcag tctggcctcg atctctcgcc gatcattacc
     5341 catcgtttct ctatcgatga tttccagaag ggctttgacg ctatgcgttc gggccagtcc
     5401 gggaaagtta ttctgagctg ggattaacac gaacaagggc tggtattcca gcccttttgc
     5461 ctgaggataa tccgttaaat atgtaaaatc ctgtcagtgt aataaagagt tcgtaattgc
     5521 actgatctct tatatagctg ctctcattat ctctctaccc tga
//
LOCUS       DACADA010000233         5445 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.236, whole genome shotgun sequence.
ACCESSION   DACADA010000233 DACADA010000000
VERSION     DACADA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.236"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            29..547
                     /locus_tag="ISB17_004493"
     CDS             29..547
                     /locus_tag="ISB17_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1982350.1"
                     /translation="MSSAAVSAAEGDESVTTTVNGGVIHFKGEVVNAACAIDSESMNQ
                     TVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAFLGTTVTSNDDTLALQ
                     SSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILPFQARYIALGQSVAGT
                     ANADATFKVQYL"
     gene            909..1619
                     /locus_tag="ISB17_004494"
     CDS             909..1619
                     /locus_tag="ISB17_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1982351.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPLIKATMQ"
     gene            1661..4312
                     /locus_tag="ISB17_004495"
     CDS             1661..4312
                     /locus_tag="ISB17_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1982352.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            4326..4856
                     /locus_tag="ISB17_004496"
     CDS             4326..4856
                     /locus_tag="ISB17_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1982353.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHTGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4869..5372
                     /locus_tag="ISB17_004497"
     CDS             4869..5372
                     /locus_tag="ISB17_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1982354.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYIWQ"
BASE COUNT         1551 a         1179 c         1210 g         1505 t
ORIGIN      
        1 cggtattatt gtcgtttctg tgttggcgat gtcgtctgct gcggtaagcg cagccgaggg
       61 tgatgaatca gtaacgacca ctgttaatgg cggtgtaatt cattttaaag gtgaagtggt
      121 aaatgccgct tgtgcgattg attcagaatc aatgaaccaa acggttgagc tgggtcaggt
      181 tcgttcttct cgcctggcta aagcgggtga cctcagctcc gccgttggct tcaatatcaa
      241 gctgaatgat tgtgatacca atgtttccag taatgcagct gttgcattcc tgggtactac
      301 tgtcaccagt aatgacgata cattagcgct gcagagttca gcggcaggct ctgcccaaaa
      361 tgtcggtatc caaattttgg accgtacagg ggaggtatta gtacttgatg gtgctacttt
      421 tagcgctaaa actgacttga ttgatggcac gaatatactg ccgttccagg ctcgttatat
      481 tgctctcggg cagtcagtag ctggtactgc aaatgctgat gcgaccttca aagttcaata
      541 tctataattc actcttaagg tgactatgcg tcatctgaaa ttgaaacagg gtgtgtgtgg
      601 catggctacg cacacccgag aaattcacca atgttcatct cattattaat gaaaaatggt
      661 ttgtattttg gagcggtatg aaaaatcaca taatatctaa acgatcaata aacaacagta
      721 gttaaacttg ctgttattta aaatatttac ttggtagcaa ggttttccta tcaaaagaga
      781 accaacattt attacaaacg cattaagtct ggaaacccgg cgactcgcca atgtattagc
      841 aatatttacc gattgtctga tgtaacggtt tccccgctta tcctcttaac acatggagaa
      901 tgcatcagat gcaaactacg cgtaccccct actctgtttc ctttatggcg acagtgttac
      961 ttttatttct ctttgcttgc catagcacca ttgccaacgc agctgtcgca cttggtgcga
     1021 cgcgggtaat ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc
     1081 ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc gggtgaccag aaagataccc
     1141 agtttgtcat taccccaccg cttttttcca tgcaaggtaa aaaagagaat acgcttcgga
     1201 taatcaacgc cactaatcat cagttaccag gcgaccgtga aagcctgttc tgggttaatg
     1261 ttaaagctat ccctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa
     1321 ttattagccg cataaaaatg ttttatcgcc ccacgcatct ggcaatggca cctgaagaag
     1381 cgcctgcaat gcttcggttt cgtcgcagcg gtagcatact cactcttatt aatccaacgc
     1441 cctacttcat taccgtaaca aatatgaaag caggaaatag caatctccca aacaccatgg
     1501 tgccgccaaa aggtgaagta tcggtggata tcccgcatgc tgtaaccggg gatatcagtt
     1561 ttcagacaat caatgattac ggtgcattaa ctccgcttat taaggcaacc atgcagtaag
     1621 ccagtgacct aattaataat aaactcaatc gggattaaaa atgacagcct ttcgtgccgc
     1681 atttaaggca tatcgtatgc atcaggtgtt aatactgcct cgctttgcac ggcttacctt
     1741 tgcccttggc ttagcaactg cggtttttcc cgttgacgca gagtattatt ttaatccacg
     1801 gtttttaagc aatgaccttg ctgaatctgt cgatttatct gcctttacta agggacgcga
     1861 ggccccacct ggtacatatc gggtcgacat ttatttgaac gacgaattta tggctagtcg
     1921 ggatattact tttattgcag atgataataa cgcagatctc atcccatgcc tgagtacaga
     1981 ccttcttgtc agtcttggca taaaaaaatc agcattatta gacaacaagg aacattcggc
     2041 agataaacat gtgccggaca acagcgcatg cacaccactt caggatcgtt tggctgacgc
     2101 atcatctgag tttgatgttg gtcagcaaca tctgtcactc tccgttccgc aaatatatgt
     2161 cggcagaatg gctcgcggct atgtttctcc tgatctgtgg gaagaaggaa taaatgccgg
     2221 gctactaaac tatagtttta atggtaattc tattaataac cgtagtaacc ataatgcagg
     2281 aaaatccaac tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact
     2341 gcgcgataac tcaacgtgga gttataacag tgggagtagc aattcatctg acagcaataa
     2401 atggcagcat atcaatacgt cggctgaacg tgacattatt ccgttacgct cacggttaac
     2461 agtgggtgat agttataccg atggcgatat ttttgacagt gtgaactttc gtggccttaa
     2521 aataaattca acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca
     2581 tggtattgct cgtggcaccg cacaagtgag tgtaaaacag aatggatacg atgtttatca
     2641 gactactgtc ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg
     2701 tgatctgcaa gtaaccataa aagaggcaga cggcagtatt cagacattat atgttcctta
     2761 ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt tatgcacttg ccatggggga
     2821 atatcgtagt gggaataacc tgcaaagctc ccccaagttc gtacaagcaa gcttgatgca
     2881 tggtctgaaa ggaaactgga caccttatgg cggaatgcaa attgcagaag attatcaggc
     2941 cttcaacctt ggtattggta aagatttagg actttttggt gccttttctt tcgatatcac
     3001 gcaggccaat acgacgcttg cagatgacac ccgtcacagc ggacaatcgg ttaaatccgt
     3061 ctacagcaaa tccttctacc agacgggaac caatatccag gtcgcaggat atcgctattc
     3121 tacgcaaggt ttttataact tatccgacag tgcatacagt cgaatgagtg gttacaccgt
     3181 caagcctcct accggagaca ccagtgagca gacactattt attgattatt ttaatctgtt
     3241 ctacagtaag cgtggtcagg aacaaataag catctctcag cagcttggac attacggtac
     3301 gacatttttc agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat
     3361 atcatttgga ttaaatgtgc cgtttggtga tattacgact tcgctgaatt acagctattc
     3421 caataatata tggcaaaacg atcgggatca tttactcgct tttacactta atgttccctt
     3481 tagtcattgg atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag
     3541 tatgtcaaac gatttgaaag gcggcatgac caacctatca ggggtttatg gcactctgct
     3601 gccggataat aacctgaatt atagcgttca ggtcggtaac acccaagggg gtaatacatc
     3661 gtctggcacc agtggttaca gttctcttaa ttatcgtgga gcttacggta atacgaatgt
     3721 cggttacagt cggagtggtg acagcagcca gatttattac ggaatgagtg gtgggattat
     3781 tgctcatgct gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa
     3841 ggctcctggc gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg
     3901 tggctatgcc atattaccat ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc
     3961 gaattccctt gcagataatg ttgaactgga tgaaaccgta gtcactgtca tcccaactca
     4021 cggtgctatt gccagagcaa catttaatgc acaaatcggc gggaaagtat taatgacgtt
     4081 gaagtacggt aataaaagcg ttccattcgg tgcaattgtc actcacggag agaataaaaa
     4141 tggcagcatt gtcgcggaaa atggtcaggt ttatctgact ggacttccac agtcagggaa
     4201 attacaggtt tcatggggca atgataaaaa ctcaaactgt attgtcgatt acaagcttcc
     4261 tgcagtctct cctggaacct tgctgaacca gcagacagca atctgtcgct aaggaacaat
     4321 agattatgaa atacaataac gttattttcc tcggtttatg tctggggtta accacctatt
     4381 ctgctttatc cgcagatagc gttattaaaa ttagcgggcg cgttctcgat tatggctgca
     4441 cagtctcatc ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat
     4501 ttccaacgac cggtagcaca agtcctgccg tcccttttca gattacgtta agtgaatgca
     4561 gcaaagggac aacgggggtg cgggttgcat ttaacggtat tgaggatgca gaaaataata
     4621 ctctgttgaa actggatgaa ggaagcaata cggcctccgg tttgggtata gaaatactgg
     4681 acggaaatat gcgtccggtg aaattgaatg accttcatac cgggatgcag tggatcccat
     4741 tggtaccaga acagaacaat attttgcctt actccgctcg tttgaagtca actcagaagt
     4801 ccgtcaatcc aggactggtg agcgcttcgg caacctttac ccttgaattt caataatcag
     4861 agtcagatat gaaaagactt cacaagaggt tcctgttagc tacgttttgc acgttattta
     4921 cagcaactct ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac
     4981 catgcactat tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acacgcaatc
     5041 tgcaacaacc tggttctgca tctggctggc acaatattac tttgtcatta accgattgtc
     5101 cgattgaaac aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt
     5161 acaaaaatga aggtactgcc gaaaatattc agatagagct aagggatgac caggatgcga
     5221 cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt
     5281 ttccacttaa ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg
     5341 cgctaatcaa tgtgatctat atctggcaat aacatcaatt tgattttatt tgcctccttt
     5401 ttaaatcaat tttttaacct taggatatat catgggtaaa acatt
//
LOCUS       DACADA010000234         5420 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.237, whole genome shotgun sequence.
ACCESSION   DACADA010000234 DACADA010000000
VERSION     DACADA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.237"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..107
                     /locus_tag="ISB17_004498"
     CDS             <1..107
                     /locus_tag="ISB17_004498"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAP1982355.1"
                     /translation="RTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            179..1537
                     /gene="mltD"
                     /locus_tag="ISB17_004499"
     CDS             179..1537
                     /gene="mltD"
                     /locus_tag="ISB17_004499"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAP1982356.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1586..2356)
                     /locus_tag="ISB17_004500"
     CDS             complement(1586..2356)
                     /locus_tag="ISB17_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1982357.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2434..3234)
                     /locus_tag="ISB17_004501"
     CDS             complement(2434..3234)
                     /locus_tag="ISB17_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP1982358.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3475..4389
                     /locus_tag="ISB17_004502"
     CDS             3475..4389
                     /locus_tag="ISB17_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982359.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="ISB17_004503"
     CDS             complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="ISB17_004503"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAP1982360.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1434 a         1300 c         1347 g         1339 t
ORIGIN      
        1 taagaacgga agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac
       61 ctgaagaacg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg
      121 tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat
      181 gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gcaccggcaa
      241 cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac
      301 aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc
      361 tttcattggc gacgagctaa agatgggaat tccggaaaac gaccggattc gcgaacagaa
      421 acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta
      481 tatgtactgg atagccgggc aagttaaaaa acgtaacatg cctatggaac tggtactact
      541 acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcgggcat
      601 ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga
      661 cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aacatgatgc agcgtctgaa
      721 caagatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg
      781 ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt
      841 accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct
      901 caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc
      961 acgtgtgcac ctgagcagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc
     1021 cgtcagcaag ctgaagacat tcaacgcagg cgtgaaaggc tccacgctgg gcgcaagtgg
     1081 tccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctggcttc
     1141 aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccac ttaacagccg
     1201 tgtttacacc gtacgctctg gcgacacgct ttcaagtatc gcttcacgtc tcggcgtaag
     1261 caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag
     1321 tttgacgatt ggtgcaggca gtagcgcaca gcgactggca aacaacagcg atagcattac
     1381 gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat
     1441 caaagatgta atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac
     1501 gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca
     1561 ccgattcccc cggtgccttt tttatttatg ccgctttatg tgcttctagc atgatgatat
     1621 cactggtgaa agagccatca ttctgcaagg caaaatacgt tttcacctct gtcgatgcgc
     1681 tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcgctaccc
     1741 atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt
     1801 cactgattaa cgtcaaccac tcaccgctgg cgtggttccg tatgtgagat gtatcgcgta
     1861 atgcttctac cgtctgtaac cagatgtcgc gcactgggtg acccggagac attacgtcca
     1921 tcacaatcag cagaccaccg ggtttcaata cccggttcac ttcacgcaat gctgcaccaa
     1981 catcatgcca atgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa
     2041 atggcaggct ttcgacataa ccctggcggg tggtgatatt tttcagttgc cgggcttcgg
     2101 cagcttgtgc cacgacatcc agcatatggg cagataagtc atacgccacc accgtgctca
     2161 cgttttgcgc agcgacaaag ctggcatgcc ctgctccgca gcccatatca agtacacttg
     2221 catcaggata atcagccaga cgcaccgcca gacgctgtaa atcttgtccg gatgcatgta
     2281 cggtactggt cagatattcg ctggcttgag agctaaactg cttttctacg tggtcatggt
     2341 gggattgtgt tgtcattgcg ctgtcctttt gttatttgag tataaaaggg cagcgccaaa
     2401 cgttggtctg ccctgtggca gacctgacat accttattta tcaggcttgc cgggactgaa
     2461 ttcaacgagt agcggattgt gatcggacgc gcgcgtaacc agtacagaag cttcgctgac
     2521 gttcagacca cggtagaaaa caaaatcgag cgggcgacca aacgcccgac ggcgctgatc
     2581 atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca aagcgatata acgcgttcat
     2641 ccttctacgg ctccaggcat tgaaatctcc cgccataatg accgggccac tgtggtgagc
     2701 tatctgatcg ccaataggaa gtaactgctt actatagaca tccacgccca gactgaagtt
     2761 gacggcgtgt atattaacca ccatcaacag gcgggtgtca ggtaacggat agaccgtcac
     2821 cagtgccgac ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc agcaatacac
     2881 tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt tgtggcagca cgaaagcggg
     2941 tacctgatcg gcggcaagat agttagcagt cgcaaactgt actaactctg gcgttgtctg
     3001 cgcttcctgc aataacacca gatgtgcatc tttgccgtag ttctttaata ccgacaacca
     3061 ttcagcgcgt tgctgtttgt atatgttcca caccaggatc cgaatacgct cttcggtact
     3121 taacggttct ccaggtggta atgcctggcc gatgctcgca aaagaccccg gcggtaagat
     3181 cctttccgca ggttgtccgg caacatagcg catggcatag gtgttttttc gcacttttga
     3241 ctttgaaacc tctatcacgt caacccttcc gcactcggga gggttctact gttataggga
     3301 ttttagctca ctctttcaac gagcaattgt atattgttat gtaagcaagt gcttacaccg
     3361 gtcaggattt tgctcattat tgagaacatt aaatcggatg gcatatctcg catcctgatg
     3421 gtttaatgac aaataaattc aaaatatctc ttctattttg caacaggagc aaaaatgaaa
     3481 gccacgtcgg aagaactcgc catttttgtt tcagtcgtcg aaagcggcag ctttagccgg
     3541 gcagcggaac aattagggca agcaaactca gcggtaagcc gggcggtgaa aaagctggag
     3601 atgaaacttg gcgttagcct gcttaatcgg accacgcggc aacttagcct gacggaagaa
     3661 ggcgagcgtt attttcgtcg cgtacagtca attttgcagg agatggcagc ggcagaatca
     3721 gaaattatgg agacgcgtaa tacaccgcgt ggactgttac ggatcgatgc cgcaactcca
     3781 gtggtgctgc actttctgat gccgttgatt aagccttttc gtgaacgcta tccggaagtc
     3841 actttatcgc tagtctcctc cgaaacgatt attaatttga tcgaaagaaa agtggatgtc
     3901 gcgatacgcg ccggtacgtt aacggattcc agcttacgtg ccaggccgct atttaacagt
     3961 tatcgaaaaa ttatcgcctc ccccgattat atttcccgct acgggaagcc agaaacgatc
     4021 gacgatttaa agcaacatat ttgcctggga ttcactgaac ccgcttccct caatacctgg
     4081 ccgatagcct gtagcgatgg acaattacat gaggttaagt acggtttgtc atccaatagt
     4141 ggggaaacac tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac
     4201 atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt taatagcaga taaagtgttg
     4261 ccagtagaaa tgccattcag tgcagtctat tacagcgacc gtgcggtaag tacgcgcatc
     4321 cgggctttta tcgatttcct tagcgagcat gtaaaaacag ctcccggagg agctgtcaga
     4381 gaggcttaat cccattcagg agccagacct tccgggctaa ccaggcggtc gttgcaatcc
     4441 agtgcggcga tcgctttttt atcttcggca tcaagctgta aattttgtgc cttaagatta
     4501 ctctccaggt tttcacgttt agtagaagaa ggaattactg agtaaccttc ccccatagcc
     4561 cacgccagaa tcacttgagc cggagtcgca ttgtgtttag ctgcaatacg agcaataacc
     4621 tcatctttca gtgccttgcc atacgccagc gtcatatagg aagtaatatg gatactgtgc
     4681 tgtttagccc aagcgaccac tttacggttt tgcagataag gagagagttc aatctggtta
     4741 gtagcgatgt tttcagcgcc aacagcagca atcgcttttt ccatcaatgg gatcgtaaag
     4801 ttggaaatac cgatctcacg cgtcagccct tgttttttgg cttccagcag cgcctgcata
     4861 aactcttcaa cagagacttc atcgtttggt gacggccagt ggattagcgt cagatcaaca
     4921 taatcggtac gcaatttttg caggctctct ttcagactcg ggatcaattt gtctttgctg
     4981 agattttcaa tccagatttt agtggtgatg tggagttcat gacgtggcac gccactttct
     5041 gcaatcgcct gacctactgc ggcttcgtta tcatagattt gtgcggtatc aattgcgcga
     5101 taatcaagtt caagcgccgt tttcacagat gaaataacaa cgtcgtcttt cagacggaaa
     5161 gtgcctaaac caaatgcagg gatagccata atatgcctct tttaatgcta ttttcgttaa
     5221 ctgaggtgat tatgggcttc agactgttgc ggaaaaatgg cgtaaaacgc agaggatttt
     5281 tgcgattttg gcaataatag aaatacaaac gaaaaaaaac ctcgagcgtt aactcgaggc
     5341 ttcttagtaa gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
     5401 gtattctaac cgactgaact
//
LOCUS       DACADA010000235         5339 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.238, whole genome shotgun sequence.
ACCESSION   DACADA010000235 DACADA010000000
VERSION     DACADA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.238"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            47..1381
                     /locus_tag="ISB17_004504"
     CDS             47..1381
                     /locus_tag="ISB17_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982361.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1397..2167
                     /gene="garL"
                     /locus_tag="ISB17_004505"
     CDS             1397..2167
                     /gene="garL"
                     /locus_tag="ISB17_004505"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAP1982362.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2197..3087
                     /gene="garR"
                     /locus_tag="ISB17_004506"
     CDS             2197..3087
                     /gene="garR"
                     /locus_tag="ISB17_004506"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP1982363.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3184..4329
                     /gene="garK"
                     /locus_tag="ISB17_004507"
     CDS             3184..4329
                     /gene="garK"
                     /locus_tag="ISB17_004507"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAP1982364.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4362..4738
                     /gene="rnpB"
                     /locus_tag="ISB17_004508"
     ncRNA           4362..4738
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ISB17_004508"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1263 a         1260 c         1491 g         1325 t
ORIGIN      
        1 ttccccataa taataaaaat cagcataagt acccgaggta aataaaatga ttctggacac
       61 cgttgacgta aaaaagaaag gcgtgcatac ccgctattta atattactga ttatttttat
      121 tgttaccgcc gttaactacg ccgatcgtgc aacgctgtct attgctggta ccgaagtggc
      181 aaaagagttg cagttaagtg cggtttcgat gggttacatc ttctctgctt ttggctgggc
      241 ctacttgctg atgcaaattc ccggcggctg gctgcttgat aagtttggct cgaagaaagt
      301 ttacacttac agcctctttt tctggtcgct gttcaccttc ctgcaaggct ttgtcgatat
      361 gtttccgctg gcctgggctg ggatctccat gttctttatg cgatttatgc tggggttctc
      421 ggaagcgcca tcattcccgg cgaacgcccg tattgtcgcc gcctggttcc cgacgaaaga
      481 acgtggtact gcctccgcca tctttaactc ggcgcaatat ttctcgttgg cgctcttttc
      541 gccgctgctt ggctggctga ctttcgcctg gggctgggag catgtcttta ccgttatggg
      601 ggtgattggt tttgtgttga cggcgctgtg gatcaagttg attcataacc cgacagatca
      661 cccacgtatg tctgcagaag agctgaagtt tatctcggaa aatggcgcgg tggtcgatat
      721 ggaccacaaa aagccgggta gtgcggcagc aagcggaccc aaactgcatt acatcaagca
      781 attgctctct aaccgcatga tgctgggcgt atttttcgga caatatttta tcaacaccat
      841 cacctggttc ttcctcacct ggttcccaat ttatctggtg caggaaaaag gcatgtcgat
      901 tctgaaagtg ggtctggtcg cctcgattcc ggcactgtgt ggctttgcgg gcggcgtgct
      961 gggaggtgtc ttctcggatt atctgatcaa acgcggtttg tccctgaccc tggcacgtaa
     1021 gctaccaatt gtgctgggaa tgttgctggc ttccaccatc atcttatgta actacaccaa
     1081 caacaccacg ctggtggtca tgctgatggc gctggctttc tttggcaaag gatttggtgc
     1141 gctgggctgg ccagtgattt ctgacaccgc gccgaaagag attgttggcc tctgcggcgg
     1201 cgtctttaac gtctttggca atgttgcctc cattgtcact ccactggtga ttggctatct
     1261 ggtaagtgaa ctgcactcct tcaatgcagc actggttttc gtgggatgtt cagcgctgat
     1321 ggcgatggtc tgctacctct tcgtagtcgg cgacattaaa cgtatggaat tgcagaaata
     1381 agcaaaggta taagcgatga ataacgatgt tttcccgaat aaattcaaag ccgcactggc
     1441 tgcgaaacag gtacaaattg gttgctggtc agctctctct aacccgatta gcactgaagt
     1501 tcttggtttg gctgggtttg actggctggt gctggatggt gaacatgcgc caaacgatat
     1561 ctccacgttt attccgcagt taatggccct gaaaggcagc gccagcgcgc cagtagtgcg
     1621 agtgccgacc aacgagccgg taattattaa gcgtcttctg gatatcggtt tctataactt
     1681 cctgattcct tttgtagaaa caaaagagga agcagagcag gcggtggcat caactcgtta
     1741 cccaccggaa ggcattcgcg gcgtctccgt ttctcaccgc gccaatatgt ttggcaccgt
     1801 ggcggattat ttcgctcagt cgaacaagaa catcactatt ctggtccaga tagaaagtca
     1861 gcagggcgta gataacgtcg atgctattgc cgctaccgaa ggtgtagacg gcatcttcgt
     1921 cggccccagc gatctggctg cggcattagg ccatctcggc aatgcctcgc acccggatgt
     1981 acaaaaagca attcagcaca tttttaaccg tgccagcgcg cacggcaaac ccagcggcat
     2041 cctcgcaccg gtcgaggctg atgcgcgtcg ttatctggaa tggggcgcga cgtttgtggc
     2101 cgtcggcagc gatctcggcg tcttccgctc tgccactcag aaactggctg atacctttaa
     2161 aaaataacca ccaccgcaac aagagagatg attgatatga ctatgaaagt tggttttatt
     2221 ggcctgggga ttatgggtaa accaatgagt aaaaaccttc tgaaagcagg ttactcgctg
     2281 gtggttgctg accgtaaccc agaagctatt gctgacgtga ttgctgccgg tgcagaaaca
     2341 gccgctacgg ctaaagcgat cgctgaacag tgcgacgtca tcataaccat gctgccaaac
     2401 tcccctcatg tgaaagaggt ggcgctgggt gagaatggca ttattgaagg cgcgaagcca
     2461 ggtacggtat tgatcgatat gagttctatc gcaccgctgg caagccgtga aatcagcgaa
     2521 gcgctgaaag cgaaaggcat tgatatgctg gatgctccgg tgagcggcgg tgaaccgaaa
     2581 gccatcgacg gtacgctgtc agtgatggtg ggcggcgaca aggctatttt cgacaaatac
     2641 tatgatttga tgaaagcgat ggcgggttcc gtggtgcata ccggggaaat cggtgcaggt
     2701 aacgtcacca aactggcaaa tcaggtcatt gtggcgctga atattgccgc gatgtcagaa
     2761 gcgttaacgc tggcaaccaa agcgggcgtt aacccggatc tggtgtatca ggcaattcgc
     2821 ggtgggctgg cgggcagtac cgtgctggat gccaaagcgc cgatggtgat ggaccgcaac
     2881 ttcaaaccgg gcttccgtat tgatctgcat attaaggatc tggcgaatgc gctggatact
     2941 tctcacggcg tcggcgcgca actgccgctc actgctgcgg ttatggagat gatgcaggca
     3001 ctgcgtgcag atggtttagg aacggcggat catagcgccc tggcgtgcta ctacgaaaaa
     3061 ctggcgaaag tcgaagttac tcgctaatga cgtcgtgccc ggatggcgta ttgcaatccg
     3121 ggcctggaat ccaggccgaa taagagaaac gccctccggc gtcatgtggt aacaggcata
     3181 ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa gtttatctgc cagcgaggtt
     3241 gcgcaggcga tagaaaaagg atttcgggaa atttttcctg atgcacagta tgtttctgtt
     3301 ccggttgccg acggtggtga aggaacggtg gaagcgatga ttgcagccac ccagggggct
     3361 gaacgtcacg cctgggttac agggccgctg ggcgagaaag tgaatgccag ttgggggatc
     3421 tccggcgatg gcaaaaccgc gtttatcgaa atggcggcgg ccagtgggct ggaactggta
     3481 cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg gtactggtga gttaatcctg
     3541 caggcgctgg agagcggtgc gacaaacatt attatcggca ttggcggcag cgccaccaac
     3601 gacggcggcg caggcatggt acaggcgctg ggggcgaaat tatgcgatgc caacggcaat
     3661 gaaattggtt ttggcggcgg tagtcttaat actctgaatg atattgatat ttccggtctc
     3721 gatccgcgct taaaagattg tgtcatccgc gtcgcttgtg atgtcaccaa cccgttagtc
     3781 ggcgacagtg gtgcttcgcg catctttggc ccacaaaagg gagccagtga ggcgatgatt
     3841 gtcgagctgg acaataacct ctctcactat gccgatgtta ttaaaaaagc gctgcatgtt
     3901 gatgtgaaag atgtccccgg cgcaggagct gcgggaggta tgggcgcggc gctgatggcg
     3961 tttcttggtg cagaactgaa aagtggtatt gaaatcgtca ctacggcgct gaatctggag
     4021 gaacatattc acgattgtac gctggtgatc actggtgaag ggcgtattga tagccagagt
     4081 attcacggca aggtaccgat tggtgtcgca aacgtggcga agaagtacca taaaccggtg
     4141 attggcattg cgggtagcct gaccaatgat gttggcgttg tacatcagca tggcattgat
     4201 gcggtcttca gcgtactgac cagcattggt acgttagacg aagcactccg cggagcttat
     4261 gacaatatct gccgtgcttc acgtaatatc gccgcgacac tggcgattgg aatgcgcaac
     4321 gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc cgaagctgac cagacagtcg
     4381 ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg ggaggaaagt ccgggctcca
     4441 tagggcaggg tgccaggtaa cgcctggggg ggaaacccac gaccagtgca acagagagca
     4501 aaccgccgat ggcccgcgta agcgggatca ggtaagggtg aaagggtgcg gtaagagcgc
     4561 accgcgcggc tggtaacagt ccgtggcacg gtaaactcca cccggagcaa ggccaaatag
     4621 gggttcataa ggtacggccc gtactgaacc cgggtaggct gcttgagcca gtgagcgatt
     4681 gctggcctag atgaatgact gtccacgaca gaacccggct tatcggtcag tttcacctga
     4741 tttacgtaaa aacccgcttc ggcgggtttt tgctttgtgt gcccagggta agatgaatat
     4801 ctacggtatt aataacagac tttgtaagaa gttattaagt ctatattgca tttaaaaggt
     4861 cgtaattata cgtatatttc tctcattaat tgtaagttcg caggtattgt ccgatcgttg
     4921 aataatttgg gcgttataac ctaaattttt accgtccaat aatcaatatt tctattgtga
     4981 ttgttaacca ctgcgtgcat tgacgggatt gaagggtgaa gaatatgtgc aattgagaag
     5041 cctttcattg atgcctgcct tgtcatcatg acaaacaata atagatgctt attctccttg
     5101 cacggttctt tcgcactatc aaaaaacgct catttacccg gcattacagt caacgaagca
     5161 acattctatg gcctacgctg atgtaaagca cagtgagaat cccccttgtt gtcttcgtct
     5221 gaccttatcg cggcgaccag caattattcg ttttgtgatc ctcaacacaa aatgctcttc
     5281 gttgcctatg atgggagaag aatttatgga aaacgattgc attcacaaaa tgacatcct
//
LOCUS       DACADA010000236         5261 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.239, whole genome shotgun sequence.
ACCESSION   DACADA010000236 DACADA010000000
VERSION     DACADA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.239"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..315
                     /locus_tag="ISB17_004509"
     CDS             <1..315
                     /locus_tag="ISB17_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1982365.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLII
                     DEIGYLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEAN
                     PE"
     gene            complement(382..537)
                     /locus_tag="ISB17_004510"
                     /pseudo
     CDS             complement(382..537)
                     /locus_tag="ISB17_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1341..1943)
                     /locus_tag="ISB17_004511"
     CDS             complement(1341..1943)
                     /locus_tag="ISB17_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982366.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(2037..2315)
                     /locus_tag="ISB17_004512"
     CDS             complement(2037..2315)
                     /locus_tag="ISB17_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP1982367.1"
                     /translation="MPVKVARCFHDHTGWFNNIRRNILMATPVSLMDDQMVDMAFITQ
                     LTGLTDKWFYKLIKVGGFPAPIKMGRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(2439..3635)
                     /locus_tag="ISB17_004513"
     CDS             complement(2439..3635)
                     /locus_tag="ISB17_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HAP1982368.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            4399..4575
                     /locus_tag="ISB17_004514"
     CDS             4399..4575
                     /locus_tag="ISB17_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAP1982369.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTANF
                     TSACWLQSCHLSVR"
BASE COUNT         1254 a         1293 c         1456 g         1258 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatct
       61 acggcacaac gtcagggccg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaat aggctatctg ccgttcgggc agtgggatca aacgttcgcc
      181 ggtgatgcag cactgacctc agcgatgctg gaccgtatct tacaccactc acatgtcgtt
      241 caaatcaaag gagaaagcta tcgactcaga cagaaacgaa aagccggggt tatagcagaa
      301 gctaatcctg agtaaaacgg tggatcaata ttgggccgtt ggtggagata taagtggatc
      361 acttttcatc cgtcgttgac acattgcctt aatctccgca aagataacct cgtcattccg
      421 gacattctct gccaggcata tgtcgatgta atccataaac gatttcagct taaccctttt
      481 gtggccggtc tttctggctg gcggttcagg ggatgttttc tggttatcgt gtaccacaaa
      541 actgggatat tgccccgata gtcacatttc agattgatcg cggtctgaag atgtggcact
      601 gaggtaacgt gctggtgtca ctggtattgc tgaagttatg gtttttcggg gtcaggtgag
      661 cctgttgtgt gtatgctttt tgttcagatc agaggggggc tgtgcggaca gcggcttttg
      721 tctccgggca ttcacgcctg aacgatgaat gcgattttca gcaatatttg tctttgtgac
      781 actctctggt tgagaagcta atgcgcgccc ctgtttccac agagaatgag gcttgccatc
      841 agccgaaaca tcagtattcc acgccataca gacggcgcct gaccatgtgc tgatgcaggt
      901 aaaatgtttc aactacaact gttcgggttg ctatgcaacg aaacagcgat tctcacgatc
      961 tttaactgca acgtttttcc gcctgaaagc tgactgcctc ctgctgaggt gggaggctat
     1021 gattcagtgc gtttatagtc tccggggtat gcatgtaccg gagcatacca tttctggtgg
     1081 ataacaagcc ggcacattca gcctgcagtg aagatggagg cacctggttg tgaagaacgt
     1141 ttctgcccgt ggctgcttcg catcatcaat ggtattcata cccgctcaga aatgaccggg
     1201 tgctgaagcc ctccgttatc gcgcagtata atgacgtata tgatctgtgg acgagtgagc
     1261 ggcgtgcctg atgtacgccg ctattactgt attttaccac agggctggtt ttctgctgcc
     1321 agccctgtgg ttatcagaca tcaactgtga gcggcatcaa ccgatgtacg gtatgccata
     1381 agcacgtact gatccatcag atttgtcagt ggttcactct gaccctgacg tgcaatgatc
     1441 cattgctcca gatctttttc tgatttgtgt ttcaggtttt tcgtccagtc gtcaaggctg
     1501 tttccggtca ttaagtcaag ccagcagagc cagaccagtt tgagacgtaa ctccgtgccg
     1561 ccaaactgac ggaagaggct gatcagtttg tttgccagag aatgttgctt caggcgctca
     1621 cattcagaca gcaggatccc ttccagcatc ggatgatgaa ccagcaggtg ttcgctggtg
     1681 cataaattca ccaccagatc atcatccagg gacggggagc tccgtttgtc cggaccataa
     1741 atcagccggt gcagactctg gggaacgatg gcaatctccg gtttttcgcc attgaatgac
     1801 acccacccgg gcgtcagatt atggaaatag tagatattcg gatgacacac gccccatgat
     1861 acggcgtgcc gggtgaagcc ggtcagccat ttctggtagt tttcatggag cagctcgtaa
     1921 cgggtctgat actgttgttt cattttcata ttcctcacgg gggataacag gcagcagagc
     1981 gccgccggca ggccggagat tgcgtctgct ttgggcgaag ggttagtggt taaaaattac
     2041 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcactttt cagccagcgg
     2101 gagctgcgcc ccattttgat gggggcagga aagcccccaa ctttgatgag tttgtaaaac
     2161 cacttatcgg ttaagccggt cagttgagtg ataaacgcca tgtcgaccat ctggtcatcc
     2221 atcagcgaaa ctggggtagc catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     2281 tggtcatgaa aacagcgcgc caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     2341 taatcgggaa tctggctgtc cggagataaa cggggggatt gcgatggcag agccgtagcg
     2401 gaatcccggg gggtgcccct attaagttgt caagcatgtt atgacgcctg cggggtataa
     2461 aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc gtcgtctcgc cagggcgata
     2521 agcgcctgat tgtgtcgttt tccctgactc attttgcggg tgtagtaagc cctggagagc
     2581 ggatccctga gcgcggcgaa ggccgacagg aacaacgccc gtttgagagc tttattaccc
     2641 cgtcgcgagg gatgctcacc gcgtattgac gagccggatc gccgagttac cggcgcaagg
     2701 ccagcataag cagcgagatg tgcgacagag gcgaaggcgc ggcaggcgac ctcggtgagg
     2761 agtctggctg cggtcctgac accgactccg ggcatactgg tcaggaccgg gtaaagaggg
     2821 tgagcaagaa ctcgctgttc taccgcaagc gccacctcgt ctctttgctt acgcagcgtg
     2881 atgagctgga gtgccagacg tggcagtact acggcagcgg cattcgtgcc gggaacgacg
     2941 acggtttgtt cggccagtgc ctgagctatg tctgctgcaa ggcgtttacc cagacgaggc
     3001 gcaagtttgc agagctgggc tgccagcttc ttctcaccca gcgaagcgag tttttctggt
     3061 gagggatatc gctggagaag atcgagtacc gccgggtgat caagtctcgg accgagaacg
     3121 cgctccagtg ccggatgtat ctgggtcaga aggccgcgga tacggttgct ggcctgcgtt
     3181 gtctgtgcgg caagatcatc atcgaagccg cagagcatgg agagttcggc gatttgctcg
     3241 tcagccagtt tcagcgtgcg tagcgcgtga ggcagggtac gggcagcttc ggcaatgatg
     3301 gcagcgtcac gagcatcagt tttagcttca ccggcgtgta agtcggctat gcggcgcatg
     3361 gccagtccag ggaggtatcc gacaaggact ccttctgagc gggcaacggc gacaggtaac
     3421 gcaccgatgg tagctggctg atcaacaacc agcagtatct gaccatgttg tttcaggtca
     3481 gatattagcg acctgagttt gttttcgtcg ttgggtaatg ctttatcgaa caggcgttta
     3541 cctgaacgat taatggctac agcgtgatgc gtatctttac cgacatcaac gccgataaag
     3601 acctggacgg attcgtaatc gctggattcg gtcattctgt ctcccttgta tatgggttaa
     3661 gcagataacc acggggagca ggtaccggca tccacgttac agacggtccc ggcaaaagtg
     3721 cctgacctga cccctattag cggttaccag cgccccacca gacccggtga catcaccccc
     3781 cggatcatgg acgactgggg gcagtaatca tgccgggtct ggctggctaa caccccatta
     3841 taaggggtac gaataaagta acgggggtgc gaaaagatgc ttccgcacgc acgcgcgata
     3901 aagaggggat agttttcgtg aggacaggga gagaagcagg cgttctgata attgcagagt
     3961 tgttttcgga ggtggttaca gtgagtcagc ccgttcctgg cgagcaggag actgatatca
     4021 ccgatgggat aaggttgtct gattggtatt ataaatataa gaagtcatca gagagtaacg
     4081 taaaggattt gccagaataa cctaagagag taaatctctc cggtaacaga gcgctgtgaa
     4141 gcattctgta tcagagtgca aatacatgaa cgctgatgcg taaaatcagc agtgacacgc
     4201 cagctccccc tatgcccatt cctgtataag acatgaactg agcgtattaa tgctcctgcc
     4261 ctgagactga gccggtgagt gaggggctga gaaatccctc ttgacactgc tctaaccaga
     4321 aatacttccc cggttgttgc ggtgcgtccg gatccccgat aacggaacac taaggctgta
     4381 ttgcgggaca ccaggtaagt ggcagcccgt aatacgtcag gagacgcggg aaaagtgaat
     4441 tcccacacgc gttataaact tacccttgcg gagctggaag cctttaactc tgccgttgac
     4501 aaccggctgg cagaactgac cgcaaatttt acgagtgcgt gctggcttca gtcttgtcac
     4561 ctgagcgttc ggtaacggag gcgcgggcgg taatagactg attttcaggt attaccggct
     4621 tacggcgtgt ggttgatatt gaggggctgt gagtttcgcg tatttgtcag aaacccagac
     4681 gagttttcct gtatgggacg aaataaggca gcggcaatac ctggaaaaat ttaatatggt
     4741 atgaaggaga acctgtcggt tacgcttaat atacccggta cactgtcagc tcaggggata
     4801 catgaaccgt tatctcacca gctacacagt atatacacac tgacgctgac agtagtcagc
     4861 aggaccacaa taatcagtgc cagcagtatg gtaacgatac ggtctgtatt ccggtaatag
     4921 ccgaaggggt cgacagaacc acaatacggg caacacactg tattatgagt aatcgcgttt
     4981 ccacagtccc tgcacttctt tttgcgtatc accatcactc cctcctcaca gccttacccg
     5041 taaccggttt tttacattaa aaaaacaacg ctgaaaattc aaccgtcagt ccggagacga
     5101 ccgttcgggt tatcacagag tgcctgagac agtgccctgc cggaggtcac ttcagggact
     5161 ctgcgcattt tttacgacgt ggctattccg tatgaaccat acggagattt aaccatgacc
     5221 tacaaataca accccttctg gcagcaacgt attcgtgaga c
//
LOCUS       DACADA010000237         5167 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.240, whole genome shotgun sequence.
ACCESSION   DACADA010000237 DACADA010000000
VERSION     DACADA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.240"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1310..1519
                     /locus_tag="ISB17_004515"
     CDS             1310..1519
                     /locus_tag="ISB17_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982370.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="ISB17_004516"
     CDS             complement(1585..2118)
                     /locus_tag="ISB17_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982371.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="ISB17_004517"
     CDS             complement(2121..3047)
                     /locus_tag="ISB17_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP1982372.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3104..3622
                     /locus_tag="ISB17_004518"
     CDS             3104..3622
                     /locus_tag="ISB17_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982373.1"
                     /translation="MRFLLILRISTEDTPCTRRPQAIYCTTTTHRKKTPGSITNMRIS
                     NIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERKLLHVLATAEKNDLQAQE
                     SERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTGELCYKKDRILQALKEL
                     KYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="ISB17_004519"
     CDS             complement(3826..4740)
                     /locus_tag="ISB17_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAP1982374.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DACADA010000238         5118 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.241, whole genome shotgun sequence.
ACCESSION   DACADA010000238 DACADA010000000
VERSION     DACADA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.241"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            8..1804
                     /locus_tag="ISB17_004520"
     CDS             8..1804
                     /locus_tag="ISB17_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HAP1982375.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAIIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QNTFYDGRDRNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTIGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1822..2490
                     /locus_tag="ISB17_004521"
     CDS             1822..2490
                     /locus_tag="ISB17_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HAP1982376.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2505..3176
                     /gene="dsbI"
                     /locus_tag="ISB17_004522"
     CDS             2505..3176
                     /gene="dsbI"
                     /locus_tag="ISB17_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HAP1982377.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGTLIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3222..4037)
                     /gene="ygiD"
                     /locus_tag="ISB17_004523"
     CDS             complement(3222..4037)
                     /gene="ygiD"
                     /locus_tag="ISB17_004523"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAP1982378.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLV
                     ELLAPVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4153..4926
                     /gene="zupT"
                     /locus_tag="ISB17_004524"
     CDS             4153..4926
                     /gene="zupT"
                     /locus_tag="ISB17_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAP1982379.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT         1315 a         1259 c         1264 g         1280 t
ORIGIN      
        1 tatatccatg tttgataaat atagaaaaac actcgtagcc ggaactgtgg cgataaccct
       61 gggtttgtca gcatcggggg tgatggctgc gggttttaaa ccagcaccgc ctgccgggca
      121 gcttggggcg attatcgtcg atccctacgg taatgcgccc ttaaccgcgc tggttgattt
      181 agacagccat gttatttctg acgtcaaagt taccgtccac gggaagggcg aaaaaggcgt
      241 tgacatcagc tataaagtgg gcaacgagtc actgaaaact tacgatggtg taccgatttt
      301 tggtctttac cagaagtttg cgaacaaagt gaccgttgag tggaaagaaa acggtaaggc
      361 catgaaagat gattacgtgg tgcagacttc cgccatcgtc aacaattaca tggataaccg
      421 ctccatctcc gacctacagc aaaccaaagt cgttaaagtc gcgccaggtt ttgaagatcg
      481 cctctatctg gttaataccc acacctttac cgcccaaggt tccgatctcc actggcatgg
      541 tgagaaagat aaaaatgccg gtatccttga tgcgggtccg gcaactggcg cactcccttt
      601 tgatatcgcg ccattcacct ttatcgtcga tacggaaggc gaataccgct ggtggttgga
      661 tcaaaacacc ttctacgatg gtcgtgaccg caacattaac aaacgtggtt atctgatggg
      721 tatccgtgaa acgccacgcg gcacctttac cgctgtacaa ggtcagcact ggtacgagtt
      781 cgacatgatg gggcaggtgc tcgaagatca caaactaccg cgcggatttg ctgacgccac
      841 tcatgagtcc attgagacgc caaatggcac ggtactgttg cgcgtaggta agagtaacta
      901 tcgtcgcgat gacggcgtac acgtcaccac cattcgtgac catatcctcg aagtcgataa
      961 atctggtcgc gttgtcgatg tatgggatct gaccaagatc ctcgatccga aacgcgatgc
     1021 actgctcggc gcgctggatg caggtgcagt ttgcgttaac gttgaccttg cccatgcagg
     1081 acaacaggca aaactggaac cagatacacc gttcggcgac gcgctgggtg tagggccagg
     1141 ccgcaactgg gcgcacgtga actctatcgc ctatgacgca aaagatgact ctatcatcct
     1201 ctcctctcgc caccagggtg ttgtgaagat tggtcgtgat aagcaagtga aatggatcct
     1261 tgcgccctct aaaggttggg aaaaaccgct ggccagcaag ctgctgaaac cggttgatgc
     1321 taacggtaag ccaattacct gtaacgaaaa tggcctgtgc gaaaactcag acttcgactt
     1381 tacctacacc cagcataccg cctggatttc cagcaaagga acgctcacca tttttgataa
     1441 tggcgatggg cgtcatctgg aacaacctgc cttaccaacc atgaaatact cccgctttgt
     1501 ggaatataag attgatgaga agaaaggcac cgttcagcaa gtgtgggaat acggtaaaga
     1561 acgtggctac gatttctata gcccgattac ctccatcatt gaatatcaag ccgaccgtaa
     1621 caccatgttt ggcttcggtg gttctattca tttgttcgat gtcgggcagc caaccatcgg
     1681 taagttgaac gaaatcgatt acaaaaccaa agaagtgaaa gtggaaatcg acgtgctgtc
     1741 agataaaccc aatcagactc actatcgtgc actgttagtc cgtccacaac agatgttcaa
     1801 ataattaaga agggaataaa aatgtcgaaa ttagggatct catcactatt taaaaccata
     1861 ctgcttaccg cagctttggc tgtttcattt accgcttctg catttaccga aggcacggat
     1921 tacatggttc tggaaaaacc tattccgaat gccgacaaaa cgctgattaa ggtatttagc
     1981 tacgcctgcc cgttctgtta caagtacgac aaagcggtta ccggtccggt atcggaaaaa
     2041 gtgaaagata tcgttgcttt cacgccgttc catctggaaa ccaaaggtga atacggcaaa
     2101 caagcaagcg aagtctttgc ggtcttgatc aataaagata aagcagcagg catttcctta
     2161 tttgatgcta actctcagtt taagaaagcc aagtttgcct actacgcggc ttatcacgac
     2221 aaaaaagaac gctggtctga cggtaaagat cccgctgcct ttatcaaaac tggtctggat
     2281 gccgcaggta tgagtcaggc ggattttgaa gcggcactga aagaaccagc agttcaagaa
     2341 acactggaaa aatggaaagc ctcttatgat gtcgccaaaa tccagggggt tccagcctat
     2401 gtcgttaatg gtaagtactt gatctacacc aagagcatca aatccatcga cgcgatggca
     2461 gaccttatcc gtgagttagc aagcaaataa cagggaaaca ggctatgggt attaagggaa
     2521 tgtggaaaga tcttcgcaca tcaccagttg acacactggt cagatggcag gagcaacgct
     2581 tgttgtggtt gttaatggcc gttgcgatgg gcacgttaat tatcctggcg cactctttct
     2641 tccagatcta tctctacatg gcaccctgtg agcaatgtgt atacattcgc tacgccatgt
     2701 ttgtgatggt gattggagga ttggttgcag cgatcaatcc aaagaacatc atcctcaagc
     2761 taattggctg cgtgatggca ttttacggca gcattttggg actgaagttt tcgctcaaac
     2821 tgaacgatat ccaccatgca gtacataatc cggatcccga ttcactgttt ggcgtacagg
     2881 gttgctctac cgatcccact tttcccttta acctgccgct ggcacagtgg gcaccaaatt
     2941 ggttcaagcc taccggggat tgtggctatg acgcaccaat cgttcctgat ggcgtcacac
     3001 tgagcagcac gcaacagtgg tttgtggaaa tgtatcagca atctgaaggc tggtatctgc
     3061 taccaccatg gcattttatg aatatggctc aggcttgtat gctggctttt ggtatgtgtc
     3121 tggtgctgct ggtcattatg agtggcgcat gggcgcttaa aatcatcaga ggctaactca
     3181 gcaacacccg aagccagtca ttctggcttc gggttctttt attaacctat ctgcaccgac
     3241 agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg ctcctgccca
     3301 tcccacgccc ctaagacata caacaacggc agatagtgct ctggcgttgg gttcgataac
     3361 gcgccacctt catggtcgag ataattcacc agaggatgtt gttccaccgg cccttgccac
     3421 gtcagattcg ctttcacata ctcattaaac gacatcgccc acggatacgg tgaactatca
     3481 ccgtgccact tcactgtacg caggttatgc accacgttgc cactggcgac caacattatc
     3541 ccttcatcac gcagcgccgc gagtttgcgc cccatttcga aatgccaggc ggcaggtttg
     3601 ctactgtcga tactcaactg caccatcggg atatcggcgt caggatacat cttaatcagt
     3661 acgccccacg agccgtggtc gaagccccag gcttctttat ccagcgtcac agggaccggc
     3721 gctaacagct caaccagatg ctgtgccagc gcaggtgaac ccggagcagg ataatgcgta
     3781 tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg
     3841 gtcactcctg ttccacgggt aaaccaatga gccgaaacca ccacaatcgc ttgcgggcgt
     3901 ggcaatgtca tccccaactt ctgccagctg cgggtataca aattatcttc cagcacgttc
     3961 atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat gatgatatcc
     4021 ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga gtaagaaccc
     4081 ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt gaagatttat
     4141 aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc agccacgttt
     4201 attggcgcgt ttctcggcgt tctcgggcaa aaaccctcga accgcttact ggcgttttcg
     4261 ctaggatttg cggcggggat catgttgctc atctcattaa tggaaatgct tcctgccgca
     4321 ttagccgccg aaggaatgtc acctgtgttg ggttatggaa tgtttatctt cggtctgctt
     4381 ggctattttg gtctggaccg catgttgcca catgcgcatc cgcaggattt aatgcaaaaa
     4441 tcggtgcagc cgttgccaaa atcgatcaag cgcacagcca ttctgctcac tctcggtatc
     4501 agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag caacctggag
     4561 ctgggatttg gtatcgccct ggccgtagcg ttgcacaata ttcctgaagg tctggcggtt
     4621 gcaggcccgg tttacgcggc aacggggtca aaacgtaccg caattctgtg ggcggggatt
     4681 tccggactgg ctgaaattct cggtggtgtg ctggcatggt taattcttgg cagcatgatt
     4741 tctccggtgg tcatggcggc aatcatggcg gcggttgccg gtattatggt ggcgctctcg
     4801 gttgatgaat taatgccgct cgccaaagag attgacccca ataataaccc cagttatggc
     4861 gtgctatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac ggcaggaatt
     4921 ggttaaagat gataatgaca gatgttgccc attgctaaag gcaacatctg tatcgttatt
     4981 aaaggtatta ttgatgcttt tcaaacactt ctttatcgga gcgtttatct aatttacttc
     5041 gggcagctaa cagcgcatct ctgtcatggc gaaaagcttt acagtaacgt ttaagatgaa
     5101 gaaaatagcc accagcta
//
LOCUS       DACADA010000239         5058 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.242, whole genome shotgun sequence.
ACCESSION   DACADA010000239 DACADA010000000
VERSION     DACADA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.242"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            612..800
                     /locus_tag="ISB17_004525"
     CDS             612..800
                     /locus_tag="ISB17_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAP1982380.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
     gene            921..1661
                     /locus_tag="ISB17_004526"
     CDS             921..1661
                     /locus_tag="ISB17_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP1982381.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(1946..2923)
                     /locus_tag="ISB17_004527"
     CDS             complement(1946..2923)
                     /locus_tag="ISB17_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP1982382.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            3709..4520
                     /locus_tag="ISB17_004528"
                     /pseudo
     CDS             3709..4520
                     /locus_tag="ISB17_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            complement(4809..>5058)
                     /locus_tag="ISB17_004529"
     CDS             complement(4809..>5058)
                     /locus_tag="ISB17_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP1982383.1"
                     /translation="TNDNVPGLLSLITAHLKDLPDDGRNEDVFKMLRSSAAILHGINN
                     LRNNYSMAHPTETLLNEADARFAINLVRSIMTYVDELL"
BASE COUNT         1330 a         1199 c         1241 g         1288 t
ORIGIN      
        1 tcataaggaa taactattgg tataaaaaaa gatgttataa aaaaaataaa aatgatcata
       61 atcaatgata gaatagttgt acaacaaacc aataccaaga tgcttttaat attcatcaca
      121 tcaaagaaaa tatcaagtcg atccagacga ctgaaatatc cccagagaac tatcaacgca
      181 atccctgata taatacttga tggtgttaat actgaaacta ttagcgtctt ccacttctga
      241 ttcattttaa cttcctggtt ttgctataaa gagagcataa aaaagagtga ataagttatt
      301 acacttcatt gtttgactcc tcgcagtaac ctttcaggaa ggttgtcgga tatttttgat
      361 aaattatgtc ataaacatat tattgtaagt aatgatttca ctaaatttat ttgatcgtcg
      421 tcataatatt tttaatctga atatagaaga tgattgtgtc atatgcagta tgatataaaa
      481 cgttaaaagt gactgaatag ataacagttc atgcatgaac aagaccataa cataacataa
      541 taaaatatgc ttattatgca taaccttatg ttgtgactaa gccgccttaa aactgagaag
      601 tagctaagaa tatggcgtca gacaccttca gttgtcgtag aaggttatct gcaggatttt
      661 tctccgtatc tgcctgttcc tggcattacc ccaattctcc caaaaacggc aaaccagccc
      721 tcagcccgcg ccacgactgg cttaatacgg gatccctgct gcaaaacata cctttacgta
      781 tttggtttta cccgcaataa tgcgtgataa gcaaacaaaa ggatccgcgc gctgcgattt
      841 attgtcgtgg aaggatccgg gcgttcaggt cagaatacct gtatccataa acagtgcgtg
      901 tgctacgtga aaaataactc atgaacaatg tcattcccct gcagaattca ccagaacgcg
      961 tctccctgtt acccattgcg ccgggggtgg attttgcaac agcgctctcc ctgagaagaa
     1021 tggccacttc cacgggggcc acaccggcct acctgctggc cccggaagtg agtgcccttc
     1081 tcttctatat gccggatcag cgtcaccata tgctgttcgc caccctctgg aataccggaa
     1141 tgcgtattgg cgaagcccgg atgctgacgc cggaatcatt tgacctggat ggagtaagac
     1201 cgtttgtgcg gatcctgtcc gaaaaagtgc gtgcgcgacg cggacgcccg ccaaaagatg
     1261 aagtgcgcct ggttccgctg acagatataa gctatgtcag gcagatggaa agctggatga
     1321 tcaccacccg gccccgtcgt cgtgaaccat tatgggccgt gaccgacgaa accatgcgca
     1381 actggctgaa gcaggctgtc agacgggccg aagctgacgg ggtacacttt tcgattccgg
     1441 tcacaccaca caccttccgg cacagctata tcatgcacat gctctatcac cgccagcccc
     1501 ggaaagtcat ccaggcactg gctggtcaca gggatccacg ttcgatggag gtttatacca
     1561 gagtgtttgc actggatatg gctgccacgc tggcagtgcc tttcacaggt gacggtcggg
     1621 atgctgcgga gatcctgcgt acactgcctc ccctgaagtg acggcatacg cgcttcctta
     1681 tataagctgt ggtcagcaga acagacataa cataagctgg agcaggtaga taagctgtag
     1741 tgagtaaacc atgttttatg aagggagcaa tgcctcagca tcaggttacg gggtgactca
     1801 cgtaagggac aggcagatgg cagctcagcc acaggcagca ctgcaggaaa ctgaatataa
     1861 acagcagtga gcagaccact cactgcacct gataatataa gctgtagtca gtaaaggagc
     1921 actcttcact gactacagtt tatgttcagc gggatttgaa gagtttttcc aggtcatcca
     1981 gtgtgatgcc cagtttttca agcagggcaa gtttctccgc catctccgga ctgacttttt
     2041 ccgcaggtga aggtggcaac ggattttcct tactctcatc attcggcgct tttaaccggg
     2101 gacgccggta gtgaatacag aagaattttg tccgcccccg ctggatctcc gtgtaatcaa
     2161 gatatccaat ctcgcgcaac tgctccattg cccgtctgac cgtctggttc tgggaaaata
     2221 caggagactt cagattgagg cgtgcacgca gccgcgccag cgatatcggt gccggatccc
     2281 ggggcaggct ctctataaag gtgtagagtg cctgggcgga ctcccgtcgc ttcagggcat
     2341 taatcgcctt aagctggaga aggacttttc tgtcaaactg gtacagttca aacaggcggg
     2401 gatcagcctg taactgaaca atatcccgtt cagtatcgta gtaggctgac tgtaccagat
     2461 gggtgatgta ttcccgggtg tgcttctcat cggtacggga aaacgagatc acggtaccgg
     2521 caatgcgctt cagggaaggg ctgatgcgct cacgcagcct gcgggatgac tggcttgaag
     2581 gtataccaca cagttttgca aactcaacaa aaggcagttc aactttgtca ccaatcacgt
     2641 tatggcgggc aaaggaatga atgatcccca cccaggtctt gaaatcgtta tccatatcca
     2701 gccgggggcc ggtgatctca accttatcga atccttctgc acgggccaga gaaagacgcg
     2761 tcagttcttc cgtggcatcg gtgcgtgaca gtgtattttt tttactgttc ttcagtgact
     2821 taagggtcgg tacaaagacg cccagacgca tcagcgccac aggttgtacg gtgttgttat
     2881 tgtttggtgt cagtgtcacc agctcaccgg acgacttatc cacctcgccg aacagttttt
     2941 tgatgtctga attttcgttt tccagaaagc ctccagcctg tggacagtaa aagaataaac
     3001 agaaaatcgt gtgtcagaac tccgctaact gactgaagaa aaacctgcag cctgtattta
     3061 acaactgagt ctggcatact gaccatagtt tatgtcatta acttacttca gtatatggat
     3121 tactgaatac agcttatcat tagcatgtta cagtttatca gtaacatgcc acagcttatg
     3181 tgatcgtagt cccggcccat gagtgacaag gtttgcagag atatggggat ctttaatgga
     3241 tcctttgttg atcattcatg gatctgttta ctggatcggg cctccggata tgtggataaa
     3301 tcattccaga aaaacagcat acactaccga ttatgaaggc ttcagggcgt acctgctcac
     3361 tgtaactaca gatccattgc gcttcctcgc cggttcgcgg gcttcgcccg gtcaccggtc
     3421 tgcggcgagc actgtttttc tgatgtctgt ctttacaggt aatttttgac ggaatctgcc
     3481 gtacttcctg agcgtacggt catatccgaa gtacacttca tatgtgtgtt tcaatgccag
     3541 ggattgtgta tacaaactac aggcaaagtc tggttatcac cggtggtagg ctgctccgat
     3601 ggaaaaattg tgaacggatt gacaccatgc cggataactc tgtcgatata agacggtcaa
     3661 agatcgtggc gttgacaagt acagcgtctg cacgccgcag agcttggcat gcaccgcctt
     3721 gatgaggtgg ttgatacgct gcttgtcctg cagaagaagc accggatacg gtttgatgtc
     3781 tggcaggtcg ttaaacggga tcacgccatt atttcgttct tcgatcaggg gatgaatcct
     3841 gcagtgccca gggttgccta ctggaccccc tttcgttacc cgctgctact gaatctggca
     3901 agcctgttcg ataatgaact ggctgaaaaa gcgtggtgtg cccgcctcga agcccatgat
     3961 ggaaggtcct catctctgtt cagtgaggtc tgcagtgagc tgcttgcgcg cgttcatacc
     4021 ctgggggacc gccgttacat tgaattgatt accgatgcgt tgtcctgggc gatgacccac
     4081 tttgacgaac tgggatataa ctgcaaaacc ggtaaacaaa agctgcagat catgcccaat
     4141 atggttggat ttcagttcgt attgcgcggt atctgttccc gcctgggagc accaaatcgc
     4201 aaagccgaca tcgttgtgga ccagcagtca cagttcaaca ccacccagcg ggagctgagg
     4261 gagttctatt accagatacg tgagatgccg tgggtacacg gtcccggttt accggtcatg
     4321 gatgtgacga acatgccggc tgaacgctgg ttttccagtc gggtacaaaa agtgcagggc
     4381 tggagctggt tgatatatat ctctggatct tcaaacgctt tatggaaggg aaggagttaa
     4441 ccagaccccc ttacccgcct ggtttacacc aatcgcaata ccggcaggac ggacagtgtc
     4501 tcccttcagt cggtggctaa acgatctaag gagtttctcg acaaactgca ggagccaacg
     4561 gcagaaatga tgaaaaaggc gagggaatac cgggatcagg aagaggcaag gcggctggaa
     4621 catcgggtgc aaatcctgcc gccatcctga tccggcaggt tgagtacaca caaaaatgga
     4681 agcggccacg acgggatgat gcgtctgtcc ggcatcttcc ggagggccag aactcgaaat
     4741 tctggccgta cttcacttct tcgttgaagt tctatctgtt cgctgtgagt ctcatatttt
     4801 tattaaaact aaagcaactc atcaacataa gtcatgatgg aacgaaccag atttatggca
     4861 aaacgggcat cggcttcatt cagcaaagtt tcagtaggat gggccatgct gtaattgtta
     4921 cgcaggttat tgatcccatg aagtattgcg gcagatgatc tcaacatttt aaacacatct
     4981 tcatttcttc catcatcagg caggtctttg agatgtgcag ttatcaggga aagcaagcct
     5041 ggaacattat catttgta
//
LOCUS       DACADA010000240         4997 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.243, whole genome shotgun sequence.
ACCESSION   DACADA010000240 DACADA010000000
VERSION     DACADA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.243"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            104..1771
                     /locus_tag="ISB17_004530"
     CDS             104..1771
                     /locus_tag="ISB17_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1982384.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEKALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1781..3040
                     /locus_tag="ISB17_004531"
     CDS             1781..3040
                     /locus_tag="ISB17_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1982385.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIACLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3051..3866
                     /locus_tag="ISB17_004532"
     CDS             3051..3866
                     /locus_tag="ISB17_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1982386.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3863..4756
                     /locus_tag="ISB17_004533"
     CDS             3863..4756
                     /locus_tag="ISB17_004533"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1982387.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         1070 a         1277 c         1546 g         1104 t
ORIGIN      
        1 gtgtgatatt catcacctta tttactcgtt gtcatcgata ccgtaatcgc cacattaaca
       61 ctgctcgtgc aattgccatg ggtgcaattt ttaaggagtt gttatgatcc acgcctttat
      121 taaaaaaggg tgttttcagg attcggtcag tttaatgatt atttcacgaa aactcagcga
      181 atcagaaaat gttgatgatg tttccgtaat gatgggtacg cccgccaata aagcgttatt
      241 agatactaca ggtttctggc atgacgattt taataacgcc acgccgaacg atatttgcgt
      301 ggcaattcgt agcgaagcgg cggatgcggg gatcgcgcag gcggttatgc agcagcttga
      361 aaaggcgcta aaacaactgg cgcagggttc aggcagcagc caggcgttga cgcaggtgcg
      421 tcgctgggac agtgccagtc agaaattacc cgatgccaat ctggcgctga tttcagtggc
      481 tggtgagtat gcggcggagt tggcgaacca ggcgctggat cgcaacctca acgtgatgat
      541 gttctccgat aacgtcacgc tggaagatga aatccaactt aaaacccgcg cgcgggaaaa
      601 aggcttgctg gtgatggggc cagactgcgg tacgtcgatg attgctgcca cgccgctggc
      661 ttttgctaat gtgatgccgg aaggcaacat tggcgtcata ggcgcttccg gtaccgggat
      721 tcaggagctg tgctcacaga ttgcgctggc aggggaggga attactcacg cgattggtct
      781 tggcgggcgc gatctcagcc gtgaagtggg cggcatcagt gcgctaacgg cgctggaaat
      841 gctcagtgca gacgagaaaa gcgaagtgct ggcatttgtt tcaaaaccac ctgccgaaac
      901 tgtgcgtctg aaaattgtta atgccatgaa agcaactggc aaaccgacgg tggcgctgtt
      961 tttaggttat accccggcgg tggcccgcga cgagaatgtc tggtttgcct cctcgctgga
     1021 tgaggccgct cgtctggctt gtctgctttc acgcgtcact gcgcgacgta acgcgatagc
     1081 gcctgtcagc agcggattta tttgcggttt gtataccggc ggtacgctgg ctgctgaagc
     1141 ggcgggatta cttgccggac accttggcgt ggaagccgac gatacccatc aacatggcat
     1201 gatgctggac gccgacggac atcagatcct cgacctgggc gatgattttt acaccgtcgg
     1261 gcgtccccat ccgatgatcg acccgacctt acgcaacctg ttaattgccg atctcggcgc
     1321 taaaccgcaa gtgcgagtgt tgctgcttga tgtcgtgatt ggcttcggcg cgaccgccga
     1381 tcctgccgcc tcgctggtga gcgcctggca aaaagcctgt gccgcgcgtt cagacaatca
     1441 accactgtat gccattgcca cggtgacagg cactgaacgt gacccgcaat gccgctcgca
     1501 gcaaatcgcc acgctggaag atgcggggat cgcggtagtg agttcgctac cggaagccac
     1561 cttgctggct gcggcgttaa ttcatccgct ctcgcctgcc acacagcaac acacaccgtc
     1621 attactggaa aacgtcgccg tgattaacat cggattacgc agctttgcgc tggagctaca
     1681 aagcgccagc aaaccggttg tgcattacca atggtcgcca gtcgccggcg gcaataaaaa
     1741 actggctcgt ttattagaac gtttgcaata aggggttccc atgtttacat cagtggcgca
     1801 agccaatgct gcggttatcg aacaaattcg tcgtgctcgt ccacactggc tggatgtgca
     1861 accggcttct tcacttatca gcgaactaaa caagggcaaa acactgcttc acgccgggcc
     1921 gccaatgcgc tggcaggaga tgaccggacc catgaaaggg gcgtgcgtgg gcgcatgtct
     1981 gttcgaaggc tgggcgaaag atgaagcgca ggcgctggca atactggagc agggggaagt
     2041 gaacttcatt ccttgtcacc atgtgaatgc cgtcgggcca atgggcggca ttacttctgc
     2101 cagtatgccg atgctggtgg tcgagaacgt gaccgacggc aaccgggcgt actgcaacct
     2161 caacgaaggt atcggcaaag tgatgcgttt tggcgcttac ggcgaagatg tcctgactcg
     2221 ccatcgctgg atgcgcgatg tgttaatgcc agtattaagc gcggcgctgg ggcgcatgga
     2281 gcgcggtatc gatctcacgg caatgatggc gcagggcatt accatgggcg atgagttcca
     2341 tcaacgcaat attgcttcct cagcactgtt aatgcgtacg ctggccccac aaattgcttg
     2401 cctcgatcat gataaacagc acattgcgga agtgatggat tttctcagcg tgaccgatca
     2461 gttcttcctc aacctcgcga tggcctactg caaggcggcg atggatgcag gcgcgatgat
     2521 ccgcgcaggc agcatcgtta cggctatgac ccgcaacggc aatatgttcg ggattcgggt
     2581 aagcgggctg ggtgaacgct ggtttactgc gcctgtaaac actccgcaag gtctgttttt
     2641 caccggcttc tcgcaggagc aggcgaaccc ggatatgggc gatagcgcga ttaccgaaac
     2701 ctttggtatc ggaggtgcgg caatgatcgc agcgcctggc gtaacgcgct ttgtcggtgc
     2761 gggtggcatg gaagcggcca gagcggtatc tgaagagatg gcggaaattt accttgaacg
     2821 caatatgcag ttgcagatcc ccggctggga tttccagggg gcgtgcctgg ggctggacat
     2881 tcgtcgcgtg gtagaaaccg gcattacgcc actcatcaat accggtatcg cccataaaga
     2941 ggcggggatc gggcagattg gcgcaggcac cgtgcgggca ccgctggcgt gctttgaaca
     3001 ggcgctggaa gcactggctg aaagcatggg gattggttga ggaacgcaca atgacgatca
     3061 tccatcctct gcttgccagc cgtagcgcac cgaattatcg ccagtcctgg cggttagcgg
     3121 gagtgtggcg gcgggcgatt aacctgatga cggaaagcgg cgaactgtta acgctgcatc
     3181 gtcaggggag tggtttcggc cccggcggat gggtgcttcg ccgcgcgcaa ttcgatgcgt
     3241 tatgcggtgg attatgcggc aatgaacgac cacaggttgt ggcacaaggt attcgtctcg
     3301 ggcgtttcac ggttaaacag ccacagcgtt attgtttgct acgtattacg ccgcctgcgc
     3361 atcctcaacc gcttgccgtt gcatggatgc aacgcgcgga ggaaaccggg cttttcgggc
     3421 cactggcatt ggcggcaagc gatccgctgc ctgctgaatt acgccagttt cgtcactgtt
     3481 ttcaggccgc gctcaatggc gttaagaccg actggcggca ctggctgggt aaaggccccg
     3541 gattaacgcc aagtcatgat gacacgctga gcggaatgct gctggcggcc tggtattatg
     3601 gcgctttaga tgcgcgctcc ggtcgcccgt tttttgcctg ttccgacaat cttcaactcg
     3661 ttaccactgc ggtgagcgtc agttatttac gttatgccgc gcaaggatat ttcgcctcgc
     3721 cactcctgca ctttgttcat gcgctgagtt gcccgaaacg taccgcagcg gcgatcgatt
     3781 cgctgctggc gctggggcat acgtcagggg cagatacgct gctggggttc tggcttggcc
     3841 aacaattatt acaagggaaa ccatgaaaac actggttgtg gctcttgggg gcaacgcctt
     3901 actccagcgc ggtgaggcgc tgacggcaaa aaatcaatat cgcaatattg ccagtgctgt
     3961 acccgcgctg gcgcgcctgg cccgttctta tcggctggca attgttcacg gcaacggacc
     4021 gcaggtgggg ctgctggcgt tacagaatct ggcgtggaaa gaggtagaac cgtatccgct
     4081 ggatgtactg gtggcggaaa gccaggggat gattggctat atgctggcgc agagcttgag
     4141 cgcacagccg cagatgccgc ctgtcacgac ggtgctgacg cgcattgagg tttcgcctga
     4201 tgatccggcg tttttacagc cagagaagtt tatcggtccg gtttatcagc cagaagaaca
     4261 agaggcactg gaagcggctt acggctggca gatgaaacgt gatggtaaat atttacgtcg
     4321 ggtggtggcg tctccgcaac cgcgtaaaat tctcgacagc gaagccatcg agttgttgct
     4381 caaagagggg catgtggtga tttgcagtgg cggcggtggt gtgcctgtga cggaagacgg
     4441 ggcagggagt gaagcggtga ttgataaaga tctcgccgct gcgttgctcg ccgagcagat
     4501 taatgccgat ggactggtga tcctcaccga tgctgatgcg gtatatgaaa actggggaac
     4561 gccgcagcaa cgtgccattc gccatgccac accggatgag ttagcgccat ttgccaaagc
     4621 cgatggttcg atggggccga aggtaacggc ggtgagtggc tatgtcagaa gccgtggtaa
     4681 acccgcgtgg attggggcgt tatcgcggat tgaagagacg ctggcgggcg aagcggggac
     4741 ttgtatttcg ctgtagtgat gggcaccagg caattggcac atctagcctg atgcgatgct
     4801 ggcgcgtctt atcaggtcta caggcggtgt cggaacggta ggccggataa ggcgttcacg
     4861 ccgcatccgg caatcggcac accatgcctg atgcgatgct ggcgcgtctt atcaggccta
     4921 cagtcggtgt cggaacggta ggccggataa ggcgttcacg ccgcatccgg caatcggcac
     4981 accatgcctg atgcgat
//
LOCUS       DACADA010000241         4990 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.244, whole genome shotgun sequence.
ACCESSION   DACADA010000241 DACADA010000000
VERSION     DACADA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.244"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            325..2610
                     /gene="nrdA"
                     /locus_tag="ISB17_004534"
     CDS             325..2610
                     /gene="nrdA"
                     /locus_tag="ISB17_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAP1982388.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2699..3829
                     /gene="nrdB"
                     /locus_tag="ISB17_004535"
     CDS             2699..3829
                     /gene="nrdB"
                     /locus_tag="ISB17_004535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAP1982389.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3829..4083
                     /gene="yfaE"
                     /locus_tag="ISB17_004536"
     CDS             3829..4083
                     /gene="yfaE"
                     /locus_tag="ISB17_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAP1982390.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4137..4787)
                     /gene="inaA"
                     /locus_tag="ISB17_004537"
     CDS             complement(4137..4787)
                     /gene="inaA"
                     /locus_tag="ISB17_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAP1982391.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1243 a         1339 c         1221 g         1187 t
ORIGIN      
        1 tttggcaatc attagcaaca agattaataa accatctata tcaatttacc tatcctattt
       61 taccgttcaa gaattcaccg aacggttatt tttaacaaat ttttctcttc ccattgactt
      121 tcccggacac cttgtctgac ctaaggtgcg cggaagccac tttttccttc ctgagttatc
      181 cacaaagtta tgcacttgca agagggccag tttcacacta tcttgcagtg aatcccaaac
      241 atacccccta tatatagtgt tcttagcagc ttccctcact acaggtagtc tgcatgaagc
      301 tattgaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa gcgcgacggt
      361 agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc ggcagaagga
      421 ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc acattcagtt ttatgacggt
      481 atcaagacct ctgacatcca cgaaaccatt atcaaggctg ccgcagacct gatctcccgt
      541 gatgcgccgg attatcagta tctcgccgca cgtctggcga tcttccacct gcgtaaaaaa
      601 gcctacggtc agtttgagcc gcctgcgctg tacgaccatg tggtgaaaat ggtcgagatg
      661 ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa gcagatggac
      721 acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa gcagctggaa
      781 ggcaaatatc tggtacagaa ccgcgtgacc ggcgaaatct acgaaagcgc ccagttcctt
      841 tatattctgg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg cctgcaatac
      901 gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt cgctgccgac gccaatcatg
      961 tccggcgtgc gtaccccgac tcgtcagttc agctcatgcg tgctgatcga gtgcggcgac
     1021 agcctggatt ccattaacgc cacctccagc gcgattgtta aatacgtttc ccagcgtgcc
     1081 gggatcggca tcaacgccgg gcgtattcgt gcgctgggta gcccgattcg cggtggcgaa
     1141 gcgttccata ccggttgtat tccgttctac aaacatttcc agacagcggt gaaatcctgc
     1201 tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct acccgatgtg gcatctggaa
     1261 gtggaaagcc tgctggtgtt gaaaaacaac cgtggtgtgg aaggcaaccg cgtgcgtcat
     1321 atggactacg gggttcaaat caacaaactg atgtataccc gtctgctgaa aggtgaagat
     1381 atcactctgt tcagcccgtc cgacgtaccg gggctgtacg acgcgttctt cgccgatcag
     1441 gaagagtttg aacgtctgta taccaaatat gagaaagacg acagcatccg caagcagcgt
     1501 gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac cggtcgtatc
     1561 tatattcaga acgttgacca ctgcaatacc catagcccgt ttgatccggc catcgcgcca
     1621 gtgcgccagt ctaacctgtg cctggaaatc gccctgccga ccaaaccgct gaacgacgtc
     1681 aacgacgaga acggtgaaat cgcactgtgt acgctgtctg ctttcaacct gggcgcaatt
     1741 aacagcctgg atgaactgga agagctggca attctggcag tacgcgcact cgacgcgctg
     1801 ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg tcgtcgtacg
     1861 ctgggtattg gtgtgatcaa cttcgcttac tacctggcga agcacggtaa acgctactcc
     1921 gacggcagcg ccaacaacct gacgcataaa accttcgaag ccattcagta ttacctgctg
     1981 aaagcgtcta atgagctggc gaaagagcaa ggcgcgtgcc cgtggtttaa cgaaaccaca
     2041 tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatgc catcgctaat
     2101 gagccgctgc attacgactg ggaagcactg cgtgagtcaa tcaaaacgca cggcctgcgt
     2161 aactccacgc tttctgccct gatgccgtcc gagacttctt cgcagatctc taacgccact
     2221 aacggtattg aaccgccgcg cggttacgtc agcatcaaag cgtcgaaaga cggtattctg
     2281 cgccaggtgg tgccggacta cgagcatctg cacgacgcct atgagctgtt gtgggaaatg
     2341 ccgggtaacg atggctacct gcaactggtg ggtatcatgc agaaatttat cgatcagtcg
     2401 atctctgcta acaccaacta cgatccgtca cgtttcccgt caggaaaagt gccgatgcag
     2461 cagttgctga aagacctgct cactgcctac aaattcgggg tcaaaacgct gtattatcag
     2521 aacacccgtg acggcgcaga agacgcgcag gacgatctgg tgccgtcaat ccaggacgat
     2581 ggctgcgaaa gcggcgcatg taagatctga taatccccca gtctttgcac caaaccgtcc
     2641 cctctcccct tgcgggagag gggacgggtg aggggtaata aaaccacagg acacattcat
     2701 ggcatatacc accttttcac agacgaaaaa tgatcagctc aaagaaccaa tgttctttgg
     2761 tcagccggtc aacgtggctc gctacgatca gcaaaaatat gacatcttcg aaaagctgat
     2821 cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt gacgtctccc gcgaccgtat
     2881 cgattaccag gcactgcctg aacacgaaaa acacatcttt atcagcaacc tgaaatatca
     2941 gacgctgctg gattccattc agggtcgcag tccgaacgtg gcactgctgc cgctgatctc
     3001 gatcccggaa ctggaaacct gggtagaaac ctgggcgttc tcagaaacga ttcattcccg
     3061 ctcctatacc catatcatcc gtaatatcgt taacgatccg tctgttgtct ttgacgatat
     3121 cgttaccaac gagcagatcc agaaacgtgc ggaggggatc tccagctatt acgatgaact
     3181 gatcgaaatg accagctact ggcatctgct gggcgaaggc acccacaccg ttaacggtaa
     3241 aaccgtaacc gttagcctgc gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt
     3301 taacgcgctg gaagcgatcc gcttctacgt cagctttgcc tgctctttcg cattcgctga
     3361 acgtgagctg atggaaggta acgccaaaat tattcgcctg attgcccgcg acgaagccct
     3421 gcacctgacc ggcacccagc atatgctgaa tctgctgcgc agcggcgcgg atgatccgga
     3481 gatggcggaa attgccgaag aatgtaagca ggagtgctat gacctgtttg tccaggcggc
     3541 acagcaagag aaagactggg cggattatct gttccgtgat ggttcgatga ttggtctgaa
     3601 taaagacatt ctctgccagt acgttgaata catcaccaat atccgtatgc aggcagtcgg
     3661 tttggatctg ccgttccaga cgcgctccaa cccgatcccg tggatcaaca cctggctggt
     3721 gtctgataac gtgcaggttg ctccgcagga agtggaagtc agttcttatc tggtcgggca
     3781 gattgacgcg gaagtggaca ccgacgattt gagtaacttc cagctctgat ggcccgcgtt
     3841 accctgcgca ttactggcac acaactgctg tgccaggatg aacacccttc ccttctggcg
     3901 gcgctggaat cccacaatgt ggcggttgag taccagtgcc gcgaaggtta ctgcggctcc
     3961 tgtcgcaccc gactggtcgc aggccaggtg gactggattg ccgaaccgtt agcttttatt
     4021 cagccggggg aaattttgcc ctgttgttgc cgggcaaaag gcgatattga aatcgagatg
     4081 tgaattggtt ttaacgccag atacgacgct tatacgtctt atctggccta caacgattac
     4141 atagcgtagt aatatgcttt cacctgctcc cagtccgctt tggggatcgg ctccagatat
     4201 ttttccagct ggcggaagtc atgattaatc gctttatcac gacgcaagcg gcgacgactt
     4261 ttctccaggt caagaaatcc agcttctgcc ttaccttctg ttttcacata gatatggcga
     4321 acataacaac agccatgctg acgattaacg ctatgcattt tcttaaacgc cagcgccact
     4381 gctttcaaca tggcttgccg tacttcgtca gaataaggcg atactgcatg ccgggcatac
     4441 cagtcagcaa tgctgatgaa ccccgccata tcttcagtca ccagcaacgc gcgccattca
     4501 ccctcaattt tcaccgcttc accaaaaaca atcttcggca cgatgacacc ggcccgttcc
     4561 agttctttaa ttaccgcaac ctcacggaca atcgttggtc ggccgaacgg ataacgtacg
     4621 gaatgaaaca gatgatgcgt catgcgcttt acatacagct ttttgccgtt gcgctcgacg
     4681 cattgcaccc cgctcatacc attacggcga tagttaggct cttcaaccca gtcgccctct
     4741 gttgcccacc agtgattaaa ttcgtcgtgt tttgctgaaa ctgccatacc ctatcgcctg
     4801 tcgtttttta ttaacgacaa tgactataga tgtttagctg aggaaaatct taataatacg
     4861 tgttgtattg acgagtatct tatgccggga acaagtcatc tcgctctaaa taattgcaat
     4921 taaggataac ccacagactt taatgaagag tggtatcgga tagcacataa gtgccatccg
     4981 gtagttaatt
//
LOCUS       DACADA010000242         4972 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.245, whole genome shotgun sequence.
ACCESSION   DACADA010000242 DACADA010000000
VERSION     DACADA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.245"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..83)
                     /locus_tag="ISB17_004538"
     CDS             complement(<1..83)
                     /locus_tag="ISB17_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982392.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADA"
     gene            246..500
                     /locus_tag="ISB17_004539"
     CDS             246..500
                     /locus_tag="ISB17_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP1982393.1"
                     /translation="MKNGIDVSVIIPAYNAAETSVSLVTKILSEPHLAIELIIVDDGS
                     TDETGNVLRQIHDDRLILIEQANQGVYAARNAALALHQGE"
     gene            1604..3679
                     /locus_tag="ISB17_004540"
     CDS             1604..3679
                     /locus_tag="ISB17_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP1982394.1"
                     /translation="MDLVDSVEAGKLTISELIDALAKDKNYSASKWDQRYREFTTLLQ
                     QTSTFSEPETDGLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYRSLRNRVFAAFSPENYTSTVDENAFSKAAEFLNQH
                     FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIA
                     STTFITSNEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            3672..4958
                     /locus_tag="ISB17_004541"
     CDS             3672..4958
                     /locus_tag="ISB17_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HAP1982395.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
BASE COUNT         1436 a         1135 c         1092 g         1309 t
ORIGIN      
        1 gcatctgcca cggtgtaatt ctggtcaacg accagttgag cggattcgcg tttaaactct
       61 gcgctgaaat ttcttttttt cattatggca cctgtgttgt tctgaggtga gcatatcacc
      121 tctgttcagg tggccaaatt cagtaaacca cttcattatt cgatatcgat gaggaaatca
      181 gggaaagaag gattatcacg atcagacgcc tcaaagatga caacgaaaga aaatcgtagc
      241 tcaatatgaa aaatggaatc gatgtcagcg tcattatccc tgcttacaac gccgctgaaa
      301 ccagcgttag tttggttact aaaatcctca gtgaacccca cttggctatt gaacttatca
      361 ttgtcgatga cggttctact gatgaaactg gcaacgttct tcgtcaaatc cacgatgatc
      421 ggctcattct cattgagcag gccaatcaag gtgtctatgc cgcccgaaat gctgctctag
      481 ccttgcacca aggagaatag gttatattcc ttgatgcgga cgacgatata gcagatgggt
      541 tcatagaaaa aagatggcag gcagttagta cgtcgcaagc cgacgtcatg atctttaatg
      601 gctggcattc ctgtgctgac caacatcgaa gagcggttca caaaaagcag ccctatggta
      661 cgcctctgtc aggttattaa tggattagac actgtgttac acaccgcgaa tggccgcatt
      721 atctgtggct gcagatggtc aggccatcct atgtcaggca gcattcactg cgcttccagt
      781 ccggcaggag ccataaagac attttttaaa cggttcatct tgcagcgagc aatggttgat
      841 tctgtacatt ggatataaag gactatatct atatcaacaa taaggcctct atcacacatc
      901 ggccggatta ctacgttgtt cgtgctataa actatgttga agtgatcgcg aaaatcatcc
      961 aagtagcgga acaagaacaa aacaaaaaat aaaggtattt ttgtaccggc atgcgctggt
     1021 agaggcacgt cattttttgg gactctaccg caatcgcgtt ggggataaat cgggcatgaa
     1081 ggactgcttc aaagcgaaca tattcgcagc ccatttattc agaggtatca gcagttttag
     1141 cgatgtgttt ttctttatta agctgctggg caagctattc tgataagttg tgcgttgcta
     1201 cacgacacca gcgcacagct taatttcacg ctttctgact aaattgttca ggccttttct
     1261 ataggtgcaa acacaactcc ccgtactttc ctcttccaac tcaaaatcat cgcaccttgc
     1321 tgacccaaga agggagcata ccctgcacta gtatccgcac agctaatact ctattgatac
     1381 caacaacgct tcagcacaac aaagtaactc gataatcaca actcaaagtg aagatgccaa
     1441 aaactcgttt gagagcaata aattcaataa catgaaaaaa atgtattttg cattgaaaat
     1501 cttacttatt gcgcttggct acaattgcat gcttggtaat atagattata aaacaccaga
     1561 ctagctgcct ggtaagtaat gtaatagcag aggaaatatc acgatggatc ttgtagatag
     1621 tgtcgaagca ggtaagctga cgatcagtga attgattgat gccctggcga aagataaaaa
     1681 ttactcagct tccaagtggg atcagcgata ccgtgaattt acgaccttgc tacaacaaac
     1741 ctcaactttt tctgaacctg aaacggatgg tttagtcaag aggctctggt atgaacgcga
     1801 taacggtatt gccagtattc gccagggcgt tccatcctta gcagaatatc agcaaagcct
     1861 gccactgctt agagaactaa ctgaacgaat tcggcaacag ccggatgaag aaacttacca
     1921 atatgttggc aatgcactac aacaagctaa agaaaacgga cttctcaagc gtatgtatag
     1981 gagtttgaga aatcgcgtct ttgccgcgtt ctcgccagaa aactacacca gtactgtgga
     2041 tgagaatgct tttagtaaag cagcagaatt cctaaatcag cacttccatc tcggtctggc
     2101 actgaccgga aattggttac agaaaaacta tgaattgaaa caagccatac acgcccaatc
     2161 tcctgacaca gatccttatt acgtgaatat ggccatctgg catctctatg aattgctccg
     2221 tgaacgcgat aatgaacaaa agcaggagaa aatagcgagc actacattca taaccagcaa
     2281 tgagcccatc gagaacaaga tcatcctaca ttcaccaact aacgtgatct tctttggccc
     2341 ccctggcact ggcaaaacct tcaggttgca gcaaaaaatg aaagagtaca cttctcatgc
     2401 tgttccagct gatcgtgatg cctggctgga ttctcgcctt gaatcgttga attggatgca
     2461 ggttataacg ctggtgctgc tcgatcttgg gaaacgagcc aaagttcgcc aaattattga
     2521 acatatgtgg tttcaacgta aggcattatt aaacggtcgt aatggcaacc tatcgaatac
     2581 agcctgggca gctttgcaat cctatacagt tcccgagtcg ttaaccgttg attataagaa
     2641 tcggcgtgag cctgccgtat ttaacaaaac agataacagc gaatggttgc tagttgattc
     2701 acagctcgag caagtggagg atttggtaga gctctacgcc gaacttaaac gaggccctaa
     2761 atctgccgaa gccatccagc gttttgcggt ggttacgttc caccaatctt acggctatga
     2821 agaatttatt gaaggtatac gcgctcgctc tgacgagagt ggcaatatct cttatcccat
     2881 tgagccgggt atctttatgc gcctttccca acgtgccaat gccgatccag gacatcgcta
     2941 cgccattttc attgatgaga tcaatcgcgg taacatatcc aagatctttg gtgaactaat
     3001 ctcactcatt gaagtagaca agcgtgcagg catgcccaat gcaatgagcc tacaactggc
     3061 ttatagcggt gatcacttca gcgtacccgc caatgtcgat atcatcggag ccatgaatac
     3121 agcggaccgt tctttagctc tgatggacac ggctttgcgc cgtcgctttg actttgtcga
     3181 aatgatgccc gatctctctt tactgagtgg agctaaggtg aaaggcatag agctcgagtc
     3241 gttgttagag aaactcaata gccgcatcga ggctctttac gatcgtgaac atacgctggg
     3301 gcatgcgttc tttatgccgg taaaaaatgc actcgatgcc ggtgatgaag aagctgcgtt
     3361 taaacaattg aagatcgcat tccagaaaaa gatcattccg cttttacagg aatacttttt
     3421 cgatgactgg aacaagatcc ggttggtgct ggcagacaat caaaagcaag acgacaacct
     3481 gcaattcgtg attgagaaaa ccgacgatct cgatacgctt tttggtaaca accatggttt
     3541 acgacaccat gatcagcaat caacagctta cgaactcaaa gatttcgatc aagagatctg
     3601 gaatattcca caggcttatc gttcaattta tcagccccag cagactcccc ttgatgagca
     3661 ggctgtaaat catgggtgaa gttatctccg tttttgaata tgacctattg gggagtgaca
     3721 aagcggcttc agttggcgca aaactggtgc ctccactggt ctttaattat ttagaagcgc
     3781 ttagcttggc aagtaatcaa gggagccagt tcctcaaact cacctctcgc tcaggcttca
     3841 aattgcttca ggttcaaaac tacgcgggga tgctttcaac acctcacggt ttccagcttg
     3901 agatactgcc caaggttggt aaaaacctca cagcggctaa tgcacgtcaa acattactga
     3961 cgatgctaag ccatttgcct gggtttcgac atattgaaac tcagcaagcc actcttcagg
     4021 ctcagcgtat gcctttgctt gagattttta ttcaccagtt tttgcatagc gttagccaat
     4081 tacttaaaca aggtttacgc tccgactatg tgagcgagca aggtaatctg gcttttatga
     4141 agggcaagct gatgctttct gcacagctgc gacataacgc ggtgaatcgc cataagtttt
     4201 gtgtcgatta tgatgactac atgcctgatt gtgcagccaa tcggctctta cactccgcgc
     4261 tagataagct tcttagtcta aagctgtcat cagagaatca acgctggctt tacgaactgc
     4321 gctttgcgtt tgacggtatt ccacttagcc gggatattga acgtgatata aacaacttac
     4381 gcctcgagcg tggtatggct cattacaacg agccaatggc gtgggcgcaa ttgatcctga
     4441 gaggaatgag tccgagtgct ttgcagggaa acaccaaagc gatatcactt ttgttcccta
     4501 tggaagcggt atttgaatcc tttgtggcac agaccctacc ctacgaatta ccttctcatc
     4561 tcaaagtaat accacaagcg gctacttatt ctcttgtgaa gcacgggtct aacgattgct
     4621 ttaaacttcg gccggatttg ttgattcagt cacgtcgacc ggtacaaacc aaattggtga
     4681 tggacacaaa atggaagcta ataaatagca actcccaaaa aacaacacag tatgggctag
     4741 cgcaagccga cttctatcaa atgtttgcct acggtcaaaa atacctaaat gggattggcg
     4801 aaatgtactt aatctatcct gcgcatgatc aatttaacca accgatacag cagcattttg
     4861 ctttctctaa aacattaaaa ctatgggttg ttccgtacca gataacagca aaccgcgggg
     4921 agaggatgat gtgggtgaag gatgtgtcca gtatataaat ttctagccaa gc
//
LOCUS       DACADA010000243         4837 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.246, whole genome shotgun sequence.
ACCESSION   DACADA010000243 DACADA010000000
VERSION     DACADA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.246"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..192)
                     /locus_tag="ISB17_004542"
     CDS             complement(<1..192)
                     /locus_tag="ISB17_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAP1982396.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGF"
     gene            complement(195..914)
                     /gene="sanA"
                     /locus_tag="ISB17_004543"
     CDS             complement(195..914)
                     /gene="sanA"
                     /locus_tag="ISB17_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAP1982397.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1064..1948)
                     /gene="cdd"
                     /locus_tag="ISB17_004544"
     CDS             complement(1064..1948)
                     /gene="cdd"
                     /locus_tag="ISB17_004544"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP1982398.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(2078..2773)
                     /locus_tag="ISB17_004545"
     CDS             complement(2078..2773)
                     /locus_tag="ISB17_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAP1982399.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2770..3168)
                     /locus_tag="ISB17_004546"
     CDS             complement(2770..3168)
                     /locus_tag="ISB17_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP1982400.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3407..4357
                     /gene="dusC"
                     /locus_tag="ISB17_004547"
     CDS             3407..4357
                     /gene="dusC"
                     /locus_tag="ISB17_004547"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAP1982401.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(4745..4828)
                     /gene="yohP"
                     /locus_tag="ISB17_004548"
     CDS             complement(4745..4828)
                     /gene="yohP"
                     /locus_tag="ISB17_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAP1982402.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         1272 a         1257 c         1228 g         1080 t
ORIGIN      
        1 gaagccgata tatgccacca tataagcgaa gaagagtccg ggatttttcg ctcgccagac
       61 atacaccggt tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc gccagccttt
      121 ccatgcttcg cggaaacaaa atatcgggat aaggaaaaaa acggcaacga caaaaaactg
      181 ctgtacatcc ataactactt tccttgtttc ttttgtaatt caagtaactg ttcgggtgtg
      241 acagcgggat acccctgcgc atcttccggc acttgatgca ttgccggaat agggaccagc
      301 ggccctaaaa aacgcggttc acgtttaaaa atataaaggt cagccagcgc accgaaacgg
      361 gcggcaaatt cgcgaatacg tactgacagc atatctttcg gtgacggtac ggcataacac
      421 tgagcctgaa tccccatatg cagcgcaata aataatgccc gctcacagtg gaaacgttgg
      481 gtgataataa tgaaatcatt agtatcgaaa actttgcgtg tacgtacgat ggaatccagc
      541 gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt cgacaccggc tgcgattaaa
      601 tctttgcgca tggtcatcgg ctcattataa ctttgcaatg cgttatcgcc gctcagtaat
      661 agataattta ccttaccgct gttataggca ttaatcgctc cttgaatgcg gtagcgataa
      721 tactgattaa ttacgccagt ccgataatat tttgctgtgc cgagcaccac accgacctgg
      781 cggtagggga gatcctgcaa ttcgtcgtag atataaggcg cggttttcca gctcatccag
      841 cgatcgaggc cgagcacagt caacagcagc aagccgatca ggactaacag gctgaggaac
      901 acgcgcttta acatgcagga gactcgatct aaggaaaatt tccatcaggc tactttaccc
      961 gctggttttg cgcaagaaac ggcaaactgt ttgccgggat tttcgacacc aggaaagggc
     1021 tggcaagccg gacaaggcgt tcacgccgca tccggcacca ggcttaagcg agaagcacgc
     1081 ggtcgatatt gtgacagccg agagctttca gcgttgcgga ggtggcatcc cactgaatca
     1141 acggcgcatc ggctttttct gccagaaccg cgcgctggat atccgggtaa tcataaccct
     1201 tgagattcaa cagaattaac gccccctgca acggcggcag agtcgggttg aatgcggcgt
     1261 tttcagcgta gctgccgctg aaaatacgac cgtctttaca ttccagcgcg acaccgcttg
     1321 gcgacttact gtaaggcatg tgcgaacggt ttgccgccgc aatcgccgcc tgagaaagcg
     1381 catcaccagt cagcgcatag ccgtgatcct gctcgtccat cagcagcgtt ttaatctcca
     1441 gatctttcgg cccaaaggca tccggtagat agtcacgcag cgcgtgtgcc tcgcgtcccg
     1501 gaagatgaat acgcagatcc agaccgctgt tcagttcatt cataaactga cggcagtgac
     1561 cacaaggcgt gtagttaacg gtgatggctg caagcgcttt ttcaccactc agccaggcgt
     1621 ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat tgtcgcacca ataaattcca
     1681 tattggcacc gaaataccag gttccgctca caccgcgcgc aattgcgcca acattaaaat
     1741 tcgacaatgg cgtacgcgca caggccgccg ccagcggaag tagtgcgaat gccagcgcgt
     1801 cttcgtccag ccccgttgcg ctcttcagcg atgagacttg ctccccggtc aacaaagcgg
     1861 ggaagtactt gtctgccaga ataggttcca gtgcagattg caagttatcc gcaagttggg
     1921 caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta atgggataac
     1981 agtttacgaa cccgctatgg ctttatatgt gatctgaatc tcattaatta tgcaacgcgt
     2041 gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta gcccattact gccagaataa
     2101 tcgggaaaag gaacggtgcg atcagcgaag taataatccc gcataacacc agcgccagcg
     2161 aactaaatgc accttcctga taatccagct cggcgcagcg cgccgtaccg agggcgtgcg
     2221 aggctgtccc cattgccaga ccgcgcgcag ctttggtacg aatacgcatc gcattaagca
     2281 atgtatgacc aaacacagcg ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg
     2341 ccggaatacc gccaatactg ccgccaactg ccattgcaat aggcgtggtg actgatttcg
     2401 gcaggattga cgcggcgatt tccggtgaag cgcccatcaa caatgccaca gaagtccccg
     2461 tcaccatcgc aaccacgctg ccgataaaac agatggtgat gatcgatttc cagcgcgcgc
     2521 ggatctggtg tagctgctca tataaaggat aggccagtgc gacgaccgcc ggttgcagca
     2581 gatcgtttag cacttcgcta cctttaaagt agctgtcgta agagatgccg gtcagcatta
     2641 aaaaaggaat gatcaccacc attgctacca gcaacgggtt aagcagggga aacttatacc
     2701 gtgccgccag tttgcgggcg gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga
     2761 tattcgccat cattcttctg atcctttctg acctacgact ttacgttcac catgcaccag
     2821 ttgtgaactc cagctcacca ccagaaaaac caccagcgta ctgactgcac aggaaaccac
     2881 taccggacca aactgtgcgc ggagcaaatc aaaatattgc atgacgccta caccaatcgg
     2941 cacaaacaat agcgccatat agcgaatcag tacgtagcac cccggattga cccatttcgc
     3001 cggaagaatt tgcaaggcca gcaggacaaa caggatcagc atcccgatga tgctgcccgg
     3061 aatggttacc ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg cataaatcag
     3121 gacaaaagca cgtaaatatt gccagataat gttcagtgtc ttactcataa tcaacacccc
     3181 agatgagatg gattcatcat acaattaatt cgtaaaacgt gctaccgatc acattaagaa
     3241 atcagcgctt attttttcgc tccccggtat tacgcaatgc gcagcgaact caggcataca
     3301 tcaagcatag agagggattg cagtttgcag caatccctct ttgcagacct ttaatccgtt
     3361 gaccggaggc gctaacatag cgcctcattt ttttgcgggt gatgatatgc gtgtgttact
     3421 ggctccgatg gagggtgtac ttgactctct ggtgcgggaa ttactgaccg aagttaacga
     3481 ctacgatctg tgcatcaccg agtttgtccg cgtggtggat caactgctgc cagtaaaagt
     3541 ctttcatcgc atttgccctg agctacaaaa tgccagccgg acaccatccg gtacgctggt
     3601 tcgcgtgcag ttgttagggc agttcccaca gtggctggca gagaacgccg cccgtgcggt
     3661 ggagttaggt tcctggggcg tggatcttaa ctgcggctgc ccgtcgaaaa cggttaacgg
     3721 tagcggtggt ggggcaacgt tactgaaaga tcctgaactc atctaccagg gtgcaaaagc
     3781 gatgcgcgaa gctgtaccgg cgcatttgcc tgtcagcgta aaagtgcgtc tgggctggga
     3841 cagcggtgag aagaaatttg aaatcgccga tgcggttcaa caggctggtg ctacggagct
     3901 ggtagtacat gggcggacaa aagagcaggg ttaccgcgcg gaacatattg actggcaggc
     3961 gattggcgag attcgccagc ggcttaatat tccggtgatt gccaacggtg aaatctggga
     4021 ctggcagagt gcgcaacaat gcatggcgat cagcggctgc gacgcagtga tgattggtcg
     4081 cggggcgctc aatattccca acctgagccg tgtggtgaaa tataacgaac cgcgaatgcc
     4141 gtggccggag gtggttgctt tgctgcaaaa atatacccgt ctggaaaagc agggcgatac
     4201 cgggttatat cacgttgcgc ggattaaaca gtggttgagt tatttgcgta aagaatacga
     4261 tgaagcaacg gaattatttc agcatgttcg ggtgttgaat aattcccctg atattgcaag
     4321 ggctattcag gccattgata tcgataagct gcgataatag tggaatcgca gcttatctga
     4381 gacaatgaat cgcttattca gcttcatcat cttgttgcgc tcctttcatg agctaagcca
     4441 catattgcca ctggcgcaag gagcgcgcag ggggaggaca atcgcctcct ccccctgcac
     4501 ccccgggctc tggcgaacaa aatcgccgct gcgcggtacc ttcagcttat ccccttcggc
     4561 taccgggtcg ggcgcgaggt aacgtccttg taagacgcgc cctcagccca catccatgtg
     4621 ggctgccccg gccttcgggg aacgcttcag cgattttgac gccaccaaca ccggagctgt
     4681 ttgcaaatca agtacacaaa atatctagtt cctgacctga caacttatat gccatatctg
     4741 atttttaaaa tatcatctta aatacgccag tcaccaccag tagcccaatc aggaaaataa
     4801 tcaatacagc ccagagtata attttcattg gcgttcc
//
LOCUS       DACADA010000244         4809 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.247, whole genome shotgun sequence.
ACCESSION   DACADA010000244 DACADA010000000
VERSION     DACADA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.247"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..845
                     /locus_tag="ISB17_004549"
     CDS             <1..845
                     /locus_tag="ISB17_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP1982403.1"
                     /translation="QQLLTLANETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQ
                     ITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKH
                     IISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAP
                     SMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGF
                     APEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWG
                     EKHE"
     gene            838..2196
                     /locus_tag="ISB17_004550"
     CDS             838..2196
                     /locus_tag="ISB17_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAP1982404.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(2185..3258)
                     /locus_tag="ISB17_004551"
     CDS             complement(2185..3258)
                     /locus_tag="ISB17_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HAP1982405.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     TKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(3270..4769)
                     /locus_tag="ISB17_004552"
     CDS             complement(3270..4769)
                     /locus_tag="ISB17_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HAP1982406.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSSAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT         1364 a         1172 c         1141 g         1132 t
ORIGIN      
        1 cacaacaatt attaacactg gcgaatgaaa cattagaaaa acaggttaaa gagcgtacat
       61 ctgccctgga attgatcaat caaaaattaa tacaggagat aaaagagcgc agtcaggctg
      121 aacaagtatt acaaattacg cgtagtgaac tggcagagtc cagcaaactg gcggcgcttg
      181 gacagatggc aaccgaaatt gcccatgaac aaaatcaacc gttagccgcc attcacgcac
      241 ttactgataa cgcgcgtact atgctaaaaa aagagatgta tccacaggtt gaacagaatc
      301 tgaaacacat tatttccgtg attgagcgga tgacgcagct catttccgaa cttaaagcat
      361 ttgcctcgcg ccatcgcgta cctaaaggtt ctgccgatgt catcaaagtg atgtatagcg
      421 ccgtggcgtt acttaatcac agcatggaga aaaataacat tgagcgacga ataaaagccc
      481 catccatgcc gttatttgtc aattgcgatg aactcggtct tgaacagata ttcagtaatt
      541 taattagcaa cgccttagat tctatggaag gtagctctta caaacgactg gatatcgcca
      601 ttcgccaggc aaataacaaa gttattatta ccattaaaga cagcggtggc ggttttgcac
      661 ctgaagttgt cgatcgcata tttgaaccat tttttaccac taaacgtaga ggaatggggt
      721 tgggactggc aatagtcagc gaaattgtcc gaaattcgaa cggcgcactc cacgccagta
      781 atcatcctga aggcggcgca gtaatgacat taacctggcc tgaatgggga gaaaaacatg
      841 aataataaat tcagtgaaat tactatagtt tatattgagg atagtgacga tgtacgtttt
      901 gcctgtgaac aaacgctcac gctggctggt tatcgcgtta tttcctgttg cgatgcagaa
      961 cacagtattc cccttattca aagtcaggcc aacattatta tactgaccga tgtcaggtta
     1021 cctggaatat caggactaga gctcctcagt tacatcaacg aaatggatag caaaattccg
     1081 gttattttaa ttaccggaca cggtgacgtg gaaatggcgg tggatgccat gcgtaatggt
     1141 gcattcgatt tcattgagaa accaagtagc tcagataaat tattgagtat tattgccaga
     1201 gcggtagaaa aacggcgatt ggttttagaa aatcagcaat tactggcgaa tctgcaacag
     1261 gaaaatggcc cggtgctgat tggtcgtagt ccgcagatgc agcaactgcg taaaatgatc
     1321 cttaatgtcg cagataccgg tgccgatgtc cttatttatg gtgaaacagg ttgtggtaaa
     1381 gaagtcgtcg ccagaatgct gcatcactgg agtacccgcc gtcaggggca gtttgttgcc
     1441 ttaaactgtg caggcttacc agaaacgttg tttgaaagcg aaatatttgg tcatgaggcg
     1501 ggcgcattta ccggtgcggt taaaaagcgt attggaaaaa ttgaacatgc aaatggtggt
     1561 acgttatttc tcgatgaaat agagggaatg ccatcaggaa tgcaggtaaa actgctacgt
     1621 gtattgcagg aaagaaccat tgaacgatta ggtgccaatc aattaattcc ggtgaattgc
     1681 cgtgttattg cagcgaccaa ggaagattta ctgcgtcgca gcgaagaaca tctgtttcgt
     1741 ctcgatctct attatcgtct gaatgtggtt agcctgaata ttccacctct acgccagcga
     1801 cgagaagata tccctgaact gttttactgg ttcgccagtc aggcggcaca aaaatataat
     1861 cgtcctctgc ctgatatttc tcccatgtta ctggcctggt tgcagtcaca aagttggcct
     1921 ggcaatgtac gtgaacttaa acacaatgca gagcgttttg ttctcggttt actgacccac
     1981 caccagccag tgccaatgac tcagcaagaa gagagtggtc ttaccgcctg cattgatgct
     2041 ttcgagaaaa agttaatcga agatatgctg cgacaaacgg aaggccaggt cagtttgaca
     2101 gcgcggctat tacagctgcc gcgcaagact ttatatgaca aactcaacaa acatcagatt
     2161 caaccgcaag tttatcgacc agaatcatcc agctaatgct gattcctgag gatgtaaccc
     2221 caaatcgata cctttgcggg cgtaatgttc aactacccag gaccagaatg ataccgggat
     2281 cgtaatcatc ttcgcattgc gttcccggtt ggcagcttct ggcataccgg ggtaaatcac
     2341 cggattgccg ccaggttgag gctgcgtagc taacagataa tccgcaaaga actgcccttt
     2401 ctcccctgcc agatcggtga attgatcagg atcgataatc aacgcaaaaa gatgattatg
     2461 cgcagatcca tccgtctgat gatcttcggc gatcgtgtca ccaccagata agatccccgc
     2521 catcatttca atcatcagtg ccaggccaaa tcctttatgc tcaccaaatg gcagtaacgc
     2581 ccctggagga ttgctgaata acaccccagg atcattgctt ggattcccct gtgcatcaat
     2641 caattgtccc ggcgcggcct gcttcccggt ggacgacatt acccgtgctt tatttagcgc
     2701 catgctactg gtggcaaaat caaccagaat cggcggacgc ccatcactga acggccaggc
     2761 aaagcagaaa gggttagtgg tcatacgcgg ttccgcaccg ccccacgggg cgaccagtgc
     2821 aggacgcccg gtgacgttac tcatggccag catcgccagg ccttgatcag caaccatttg
     2881 cccgtaatgc cccatacgcc ctaaatgatg cgcatggcac aacgttaaca ggcaaaaacc
     2941 gcggtgtttt gtttgttcga tggcagcatt taccgccact tttccggcgt gttgaccaaa
     3001 gccattgtgg gcatgaaaac gctgtaaatt accttcactt gttaagcatt ccgggcgggc
     3061 attcgcggtt acatcgccac gagcgatatt ttccagatat ttcggtagca gcgtgacgcc
     3121 gtggctggca aagccataac gttccgcttc aatcatatgt tccgccacat cctgagcgat
     3181 atcggcatca cagccattaa gttccagcag cccggcagct aaccaacgca ctttttcaac
     3241 agcaatttgt aatgacatcg ttggtttcct cacattagac ctaaaatttt ccaccaggtg
     3301 gcggcaaaca gtagcaacaa caggtagccg attaccgtca ggtacagtcc cacacgggta
     3361 aactggcgtg gcgtgaaggt atccgtaccc atacacacca tattttgtgg cgcgttaatc
     3421 ggcaggatga agccaaaact gacactgaag gcgagcagaa tggtcatgcc gaccgggtta
     3481 acgccgagtt ctggcggcag actgcttaac aggctaatca ggatgggcag caatgcggca
     3541 gttagcgcag tggcactggc aaaccccagg tgaatgatta tcaagaaagc ggcgagaatc
     3601 gcgaagatgg caagtgatgg taaaccgtca agaccaaaac ctttcaccac atagttcgcc
     3661 atccatgaag ctgcctgtgt atcaagcaac gtggacccca ggctgatacc aataccaaac
     3721 atcagcaacg tgccccactg cacacgtttt tcgacctctt tccagctcat tacaccgatg
     3781 cctggcagca acataatcgc cagtccggca agggtgacag aggtggtatc aatactgtgt
     3841 aattttccgc cagtagacca aaagagcagt aacaacagcg aaataccgat caagcgtttt
     3901 tctttaccgg tggttggccc taactctgcg agtgcttttt taatggcctc gctcccccct
     3961 tcaacagctt ccgtttctgg cggcaacaat ttacgtgcga ggaaatagag gatcaccgac
     4021 atggtgaggc tccagggcgc gcccgctaac agccagtcaa gccagctgac agaatgcccg
     4081 gcgccgaaag ttttattgat aaaaccgata gaaagcaggt tttgcgccga cgaagtttga
     4141 ataccaacgt tccagatact ggtcgcctgg gcgataacaa tcatcattga cgccgctaaa
     4201 cgtgaatgtt tatccacctt aaatgccgca atgaccccca tcataatcgg caccacgcaa
     4261 gccgtacggg cagtggcact gggtacgacc aggctcagga cgatggtcac cacaatggca
     4321 ccgataataa tgctgcggct actggcacct attttcgaca tagtaaacag cgcaatacgt
     4381 ttatccagcc cggtaatggt cattgccgcc gcgataaaca ttgctgctgc caccagcgcc
     4441 agagcagaat tagaaaaacc cgacagagtc attttcagcg ctttcgcggt gccaaggatc
     4501 gtgtccgggt gattcatatc cggggcaaaa ccaaccataa agataatcag tgcggaaata
     4561 acaattgcac tggcggtgta atccatcgct tcgctgatcc agacaataat ggcaaacacc
     4621 agaatggcga tcatattttg tccggcgacg ggcaaggtat cgcccagagg cagaagagta
     4681 ataataattc cggcaatgat cgccaaccac aggccgggat tagggataat tcgacgcttt
     4741 tgtggtgcgc catgtgatat tgatgacata ttcacctcat tgtgaaaaat aaaaaacctg
     4801 cctggctat
//
LOCUS       DACADA010000245         4808 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.248, whole genome shotgun sequence.
ACCESSION   DACADA010000245 DACADA010000000
VERSION     DACADA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.248"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            104..571
                     /locus_tag="ISB17_004553"
     CDS             104..571
                     /locus_tag="ISB17_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982407.1"
                     /translation="MLKTNSLRESMLHGCRWCQANPEKFTIFVESGNIETTGEAPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            564..1199
                     /locus_tag="ISB17_004554"
     CDS             564..1199
                     /locus_tag="ISB17_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP1982408.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTEWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1196..1777
                     /locus_tag="ISB17_004555"
     CDS             1196..1777
                     /locus_tag="ISB17_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1982409.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNT
                     TRAGAFNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKEGKMSGNI
                     THSGGNFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            1774..2124
                     /locus_tag="ISB17_004556"
     CDS             1774..2124
                     /locus_tag="ISB17_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1982410.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            2128..3024
                     /locus_tag="ISB17_004557"
     CDS             2128..3024
                     /locus_tag="ISB17_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1982411.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSASIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3017..3547
                     /locus_tag="ISB17_004558"
     CDS             3017..3547
                     /locus_tag="ISB17_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1982412.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGARITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            3550..>4808
                     /locus_tag="ISB17_004559"
     CDS             3550..>4808
                     /locus_tag="ISB17_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982413.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVVLTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLTTAAGKF
                     PYFTGNDVASLATLTKVGRDILAKSTVA"
BASE COUNT         1178 a         1334 c         1383 g          913 t
ORIGIN      
        1 taactgatat cactaaaaat gccgcgtacg gcgtcgatat cggcagcctg gtgctggaaa
       61 tcaataatcc cgcagcataa ggaagagcag gagagcaaaa cagatgctta agacaaacag
      121 tctgcgagag tccatgcttc atgggtgtcg gtggtgccag gctaatcccg agaaattcac
      181 cattttcgtg gagagcggca acattgaaac gaccggagaa gcgccctcgt ttgtttaccg
      241 ctatcagatg gtgatgtttg tcatggatta cgccggagag ctggacgacc tcacgctgcc
      301 gctgctggcg tggttatccg aaaatcagcc acagttgttg ctcaatccgg agcgtaatca
      361 ggacatcaaa ttttctgccg ttatcaatga cgatgacagc gccgatctcc tgtttacgct
      421 ccccctgcgg gaacgcgttc gcatcacgcg caacagtcag ggcacaccgc aggcagaaca
      481 cctgccggag ccaaaacccc gtctgccttc ttccgaaggc gactggtcgc atgtattcca
      541 ggatgtgacg tggggtgaaa gcgatggata aggcattcac ccgcgtggat gaaacctttg
      601 aggctatccg cgacagcctg aatcagcagg ccatcaataa catcgccaga aagctggcac
      661 aggatttacg tcgcgcccag caggcacgta tccggtcaca aaaagcgccg gacgggaccg
      721 aatggacacc acgcagacgc cgcgtaaccc ggatacagga gcgcattcgc tttatctgga
      781 ataacgaagc acgcacgctg aaaaactggc atcacgacac agggaaatac gggcgaacca
      841 ttaccgggtg ggatgaggat aaaaacaata tccgcacgtt ttaccgggat gacatcgacc
      901 gttttctgga aatacgcacc cggcgcatca accaggacag cacaaagcgc gtccccatgt
      961 tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc agatgcctcc ggtgtgacgg
     1021 tgggttacag cggcgtggcc gcacgtattg cacgcgttca tcagttcggt gagcgcgatc
     1081 aggttgcgcc gggcattttc accgattacc cggtacgtga gctgttgggt atcagccagg
     1141 cagatgagcg cctgatttat aacacggtgc tgggccggat tgcggaggct gtacggtgag
     1201 cgcagaactc atgcgactgc tgagcaacat catccgcacc gggatcatct ctgaagttga
     1261 tgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg gaaacaggct ggttgcgctg
     1321 gaacaccacg cgcgcgggag ccttcaatgt gtggctgccg ccatcaccag gcgaacaggt
     1381 ggtaattgcc tgcattggcg gcaacccgga aaccgccatg ataattggca gcctgtggag
     1441 tgatgccagt ccggcccccg gcaaaagcct gaaagaaatc gtggtcagcg cgccggatgg
     1501 cgcggtgttc cgctacgacg cagacgctgg cgcactgagc gccagcggca tgaaaacagc
     1561 caccctgcag gcatccgtca gcgtgacact ggatacgccc gtcgtggaat gcacaaacct
     1621 tctgagaacg gcgacgcttg acgtcacaaa agagggaaag atgagcggca atatcacgca
     1681 cagcggcggc aatttcacct caaacggcat taccgtgcat acgcataagc acggtggcgt
     1741 taaaggtggc agcgattcga caggaggccc gcagtgacaa cccgctacac aggaatgaac
     1801 ccggacggga cgggaaacct gaacgatatg gagcacctga aacagtcagt cagggacatc
     1861 ctgatcaccc cgctggcaag ccgggttatg cgacgggaat atggcagcct tgtgcctgat
     1921 ttgattgacg aacccatgaa taacaccacg cgtctgcaat gcatgagtgc tgccgtgatt
     1981 gcgctgacac gatgggaacc ccgcattgcc ctggacgcca tcgacgttgt ctggaaggca
     2041 ggaggccgcg ccggggtgac gctgtcgggc actgtcatgc agaccatgca gaatgttgaa
     2101 ttaaccatca cgctgagaga gtaaatcatg cctgctgttg atctttccca gttaccggaa
     2161 cccgccatca tcgcggagcc tgattttgag gcaattctgg ctgacacaaa ggccatgatg
     2221 attgcgtcct atcctgccga acagcgtgaa gccgtctccg ccgcgctgga gctggaatca
     2281 gaacccctga acgttatcgc tcaaaccatg tcgtttcgtg aaatgctgtt acgccagcgg
     2341 gttaacgagg gcgcacgcgc ctgcatgtta agccacggtt cagggacaaa cctggacaac
     2401 ctcgcgggca atatgaacac aaagcgcctg gttatcactc cggcaacgga taccaccgac
     2461 gcggtgatgg agagcgacac ctcgctgaga ctgcgggcgc agcgggcgta tgacggcctg
     2521 agtgttgctg gcccgtcagg tgcatacgag tattttgcac gcagcgccag cggtctggtg
     2581 cgtgatgcgc gggccatcag cccgtctccg gcctgtgtga cggtttccat tctgtccact
     2641 gaaggcgacg gcacagcaac ggaggcgttg cttaataccg ttcgcgccgt tctgaatgca
     2701 gaggataccc gcccggtggc cgaccgactg accgtacaga gcgccagcat cgtgacatgg
     2761 cggctgaatg caaaactgta cttttacccc ggcccggaat ccgaacctat tctggctgcg
     2821 gctgaatcgt cgttcaggaa gtggctggct gagcaggggc ttattggtca ggacgtggcg
     2881 ttgtcagcca ttgctgccgc acttcatgtg cacggcgtgc aacgcgtgga gataatcgaa
     2941 cccacacaga atatggccat cagcgacata caggcggcgc gctgtgagtc attcaccatc
     3001 agcgaaggtg ggcgtaatga gtaattcact gttaccgcca tcagccagca atttcatgcg
     3061 ttgcgccgaa gctgtcggag cgcgcattac agacatcccg gtagacctca acacgctgtg
     3121 gtcaccggac acctgcccgg tgcatctgct gccttatctc gcctgggcat tttccgttga
     3181 ccgctgggat cgcaactggc cggaagagac aaagcgacag gtgattcgtg atgcatggct
     3241 gatacaccga cacaaaggga ccatcagcgc actgcgccgg gccattgagc cgctgggata
     3301 cctcattcgc gtgtctgagt ggtgggagtt cggcggagaa ccgggaacat ttaccgttga
     3361 agtcggcacg ctggacagtg gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat
     3421 tgctgatgcc cgcccggtca gccgccacat gacagggctg aatatcattc aggagattca
     3481 gggagatatt ttcgcggcgg cagcaactta cgacggcgaa gtcattacca tttatccgga
     3541 cgattaagca tgagtaccac aacacgtaaa tttaaaaccg ttatcaccga tacgggtgcc
     3601 aaaaaattag ctcaggcagc cgcgccagat ggtaagcctg tccgcctgac tcatatggcc
     3661 gtgggcgacg gtggcggcac gttgcccaca ccagacagta agcagacccg tctggtgcat
     3721 gaggtgtggc gacatactgt taatcgcgtc atcctggacg caacacatca gaaccgcatt
     3781 attgcggagc tggttattcc tcctgaaacg ggcggattct ggatccggga aattggtgta
     3841 tttgatgagc acggcgattt aatcgcggtg ggcaatactg ccgaaagtta caagccagcc
     3901 gttgccgaag ggtccggtcg tgcacaaaca tttcgcacca ttctgaccgt atccagcact
     3961 gccaccgtgg tgcttaccgt ggataacacc atggtgatgg ccacagtgga ttacgtggat
     4021 gacaaactga aagagcatga acagtcacga cgtcacccgg acgcctcgct gaccgcaaaa
     4081 ggctttgttc aactcagtag cgccactaac agcgtgtctg aaacgcaggc tgcaacgccg
     4141 aaagcagtaa aggccgcgta tgaccttgct aacggtaaat atactgcgca ggatgccagc
     4201 acgacgcgaa aaggccttgt ccagctcagt agcgccacca acagcacgtc tgaaacgcag
     4261 gctgcaacgc cgaaagcagt aaaggccgcg tatgaccttg ctaacgcaaa atataccgct
     4321 caggacgcca cgacggcaca aaaagggata gtccagctca gtagtgccac caacagcacg
     4381 tctgaaacac tggccgcgac atcgaaagct gttaaggcgg taatggatga aacgaacaag
     4441 aaagcgccct taaatagtcc tgcactgacc ggaacgccaa caacaccaac tgcgcgacag
     4501 ggaacgaata atacccaaat cgcaagcacg gctttcgtta tggctgcgat tgccgccctt
     4561 gtagattcgt cacctgatgc actgaacacg ctgaacgagc tggctgcggc gctgggcaac
     4621 gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc gaaagatgcc
     4681 accctgacgg cgctggccgg acttactact gcggcaggca agtttccgta ttttacgggg
     4741 aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct tgcgaaatcg
     4801 accgttgc
//
LOCUS       DACADA010000246         4734 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.249, whole genome shotgun sequence.
ACCESSION   DACADA010000246 DACADA010000000
VERSION     DACADA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.249"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(96..857)
                     /gene="kduD"
                     /locus_tag="ISB17_004560"
     CDS             complement(96..857)
                     /gene="kduD"
                     /locus_tag="ISB17_004560"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAP1982414.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(887..1723)
                     /gene="kduI"
                     /locus_tag="ISB17_004561"
     CDS             complement(887..1723)
                     /gene="kduI"
                     /locus_tag="ISB17_004561"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP1982415.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2010..3191)
                     /locus_tag="ISB17_004562"
     CDS             complement(2010..3191)
                     /locus_tag="ISB17_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP1982416.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3446..4675
                     /locus_tag="ISB17_004563"
     CDS             3446..4675
                     /locus_tag="ISB17_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1982417.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1236 a         1205 c         1046 g         1247 t
ORIGIN      
        1 tatggattat ttcataacca tgatatggat tatgatgatc tacaggtata aaaaaccctg
       61 ccatgcgaca gggtcataaa agtaagagga atgaattaac gcgccagcca accgccatcc
      121 acggcaatgg tataaccatt cacataatct gaagcgctgg aggcaaggaa caccaccggc
      181 cccatcaggt cactcggcag tccccaacgg ccagctggaa tgcggtcgag aatttccgcg
      241 ctacgttgtt catctgcccg cagttgttga gtattgttgg tcgccatgta acccggagct
      301 atcgcattaa cattaatgtt gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc
      361 atcacaccgc tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc
      421 attgacgcga tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct
      481 gcctgagaca tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt
      541 tcgctgaatt cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca
      601 atatgaccaa actccgctac cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc
      661 agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg tgacctgctt gatggtttca
      721 gtcggttcaa cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc
      781 atcccctggc ccagcccagt atcacaacca gtgacgaccg caactttacc ttcgagagaa
      841 aatgcactta aaatcataac aataccttat tcgtttatgc ccacaactag cgcaaatctt
      901 taacggccac atggtccata tcatcaaaaa cctggttttc accgaccatg ccccagataa
      961 aggtataagc tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct
     1021 gctcgttatg catcacaatg tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc
     1081 aggcgtcatc atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac
     1141 acggcatggt gttccacaag ttacctggag ccagctccgt cagccccata ctcaattggc
     1201 aggtttccag cacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt
     1261 tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg
     1321 tatgcgcggg tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa
     1381 agacaacttc ttttgcacct ttaccaacat acagggcgtc gcggtgaccg atttcatagc
     1441 attgaccatc gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac
     1501 gttcaaggaa atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt
     1561 tagttaccgg cataatgccg ccaacaataa tacggtcaat gtggctgtaa accatggtgt
     1621 actcatcggc gacaaatacc ttttcaacca aaaattcatt gcgcagcccc tgggtatcca
     1681 gcgtttttgc gtgcgcgctg tggatgctct gtcttacgtc cacatcaacc tccgataatc
     1741 agtgaagcgt gaaaatagaa aacaataata aaacactgtt tcgttttctc tatgagggga
     1801 tgatatgccc atgggatagg gttttcaatt aaaaataaaa caatgtttca ttttcaagtt
     1861 aaggattaaa aaaagtgcag tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg
     1921 agccctgaaa aggtatgtcg actcaccagc catcattatt caacagatta atgacggcag
     1981 atatttatcg gtaaggaggt aatgcagagc tactcgtcac gttcaatggt caatgccaca
     2041 ccctggcctc cgccgataca aagcgttgcc agtcctttgc gggcattacg tttcaccatt
     2101 tcatgaacaa gagaaaccag gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg
     2161 atcgcaccac cattaacatt gacccgacgc tcatcccatt caagcatctt gccaaccgaa
     2221 agcgcctgtg cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag
     2281 ccaacacgct ccaggcaacg gcgggtcgca tacaccggcg caattcccat caatgccgga
     2341 tccacaccaa cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt
     2401 gctttggctt cgctcatcat cattactgca gctgccccat cgtttatgga tgatgcatta
     2461 cccgctgtca cagaaccgag actatcaaat gaaggattta aacgggctaa gccttctgcg
     2521 ctggtgtcag tgcgtggctg ttcatcggta tcaacaacca acgtctgacc gttactttgg
     2581 gttattaccg ggacgatctc atctttaaat cgtccggcgt caatcgccgc tcgcgctttt
     2641 tgttgcgagc taagtgcgta agcatcctgc aactgacggc tgatgccata ttcgcgagcc
     2701 agattttcgg cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcatgc
     2761 acaagactat caaccaactg gctattgcca agctgtgcgc cggtgcggct atcagtcaga
     2821 acatgtggtg cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca
     2881 cactgtatcg cctgagtagc cagatgcagt gctttaagcc cggaaccaca aacgtcatta
     2941 atagtgattg cagaaacgct attaggaaga ccacctttaa tagccgattg tcttgccgga
     3001 ttctgccctg cccctgcagt caacacctga ccaagaatca cttcatccac cgcatatgca
     3061 ggaacgccgg tacgttctat taacgctttg acgaccaggc tgccaagttc cacggcggaa
     3121 tgacccgcta acgcaccacg aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca
     3181 acgtctttca tcacaacctc gcaccaatta acagtgcaat agtaaatgat tgttaattac
     3241 agttatctta attgtttaaa aaaagtgacc attatcacaa aggaaatacg cctgagtagt
     3301 agtcagagac ataactggca cgtaaggttt gcaaccacta acccaccaat agagaggtag
     3361 ataaggcgtt aatctcccat acttaacctg gtttatggta aattgccctc cattttgttt
     3421 aatttgtaga tgatacgttc agataatgtc taatatttgg tcaaaagaag aaactctgtg
     3481 gagtttcgca ctctacggca cagccgttgg tgcaggcacg ctcttccttc ctattcagtt
     3541 aggttcggcg ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata
     3601 ttggccacat aaagccttat gccagttcat cctctcatcg aaaacatcag caggtgaagg
     3661 gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct
     3721 gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact
     3781 tacggaacag ctggcaaagc atatggttat tgatcttcgc atccgtatgc tagtgagtct
     3841 gggggttgta ttaatcctga atctcatttt tctgatggga cgccatgcca ctattcgggt
     3901 aatgggattt ttggtattcc cattgattgc ctatttctta tttctttcta tttacctggt
     3961 cggtagttgg caacctgatc tattaacaac ccaggtagag ttcaatcaga atacccttca
     4021 ccagatatgg atatcgattc ccgtgatggt tttcgctttt agccatacgc ccattatttc
     4081 tacgtttgcc atagacagac gtgaaaaata tggcgaacat gctatggata aatgcaaaaa
     4141 aattatgaaa gtcgcttatc tcatcatctg cataagtgta ttgttctttg tctttagctg
     4201 cctgctttct attccacctt cgtatattga agcggctaaa gaagaagggg ttaccatttt
     4261 atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt
     4321 cgcagtagtt gcgatgtcga aatcattcct gggtacgtac tttggcgtta ttgaaggtgc
     4381 cacagaggtc gtcaaaacaa cattacagca ggttggtgta aagaaaagtc gtgcatttaa
     4441 ccgcgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc
     4501 gaacgcgatt tcgatgattt acgcgatcag cggcccgctc attgccatga tacttttcat
     4561 catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa
     4621 tctgattacc ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta gctaatatcc
     4681 gttgtggatg gttcaaaatt gcttcaattt tgaaccatcc cagttttcat aatg
//
LOCUS       DACADA010000247         4610 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.250, whole genome shotgun sequence.
ACCESSION   DACADA010000247 DACADA010000000
VERSION     DACADA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.250"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..208
                     /locus_tag="ISB17_004564"
     CDS             <1..208
                     /locus_tag="ISB17_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAP1982418.1"
                     /translation="GYGVLWALMVLLMNMHDVSESVAGLAIALLTLMVCSSAGERVKR
                     FWPRQFFVMFYAVHLAVLGIVVSM"
     gene            280..873
                     /locus_tag="ISB17_004565"
     CDS             280..873
                     /locus_tag="ISB17_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAP1982419.1"
                     /translation="MAEEKRPVLSLKRKPAENSTAPAEATPAPGVVRRKKVVVVSSPP
                     AWKAKKAKLEKAKQAAEADARNAAPAPDKAVKTPPPVRYLRLLPPEQAIMTLKAFWPQ
                     LFDGNSPRLLARGMREQLFADIVNRDLPLSHKQVIKCLKSLTRSADYLSRMKVGASRY
                     DLQGNAVATVTAEEAQYASERMMKELLRAERKMSQTL"
     gene            1034..1636
                     /locus_tag="ISB17_004566"
     CDS             1034..1636
                     /locus_tag="ISB17_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982420.1"
                     /translation="MTMTEKIIALVDYENIGTLENVRLSRYERLILFTGPQQEFIRFP
                     AVTYAGDISVSVFQVSNVSKNNVDFHLVFELGRLSATAPEETIFHIISNDKGYDGVIA
                     ELCRAGRRCCRISSPCSAEKPKAAPMVISNDEVLHLTDKVQSLSKKSAGNRPANTSSL
                     MNYIKSLSGNTKGMALYGRVKEELIRRGVIAVYEKTVVWR"
     gene            1686..2330
                     /locus_tag="ISB17_004567"
     CDS             1686..2330
                     /locus_tag="ISB17_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982421.1"
                     /translation="MFRYFILRPEQQLFCYLYGCALALVQMVLFSPVSRASGFYLVAL
                     SVALFWAGLALYTRHIDRMRKPEVSPLVSIRDGIQVVAEVPRYEKARLEWEILRDDEV
                     FRQQRCELTGLTGRVISRGLLYTPAVMLVGIGILAWGSPQDAIRLINALRNMPAAELV
                     HQIGFVLCHFLQISVISVLIADVVAGRGLPNVFRRALLDRLPAEFCLIRRGTER"
     gene            2386..3036
                     /locus_tag="ISB17_004568"
     CDS             2386..3036
                     /locus_tag="ISB17_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAP1982422.1"
                     /translation="MAKILMMAGSTVVVLSLLGFLVLLLKGRAVTKTSPVLRSLKALG
                     IRPSEAEQRLCRQRVWVGNDTLMTPREQHFFRALLRHTSRTRWLLCPQVRVADIATLS
                     PHIRPRSRTWWQLFRMASQWHCDVVIVDIHTFAIIGAVELDDASHQKKHRIRRDILLE
                     EVLRQAGIPLLRDRDSESLVRKVVEFLKYREAGAEEKSITVMKSTTKHSEHKEKEK"
     gene            3033..3341
                     /locus_tag="ISB17_004569"
     CDS             3033..3341
                     /locus_tag="ISB17_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982423.1"
                     /translation="MIIAAEVKRDEQGYWTHPALVRSGCETSAELSYWLRTHKLQCFV
                     MTMRDEAPEAFAAGFTDEAPDARGWIPEPPDDDGWFLGSVHDTGDGPVCYWFRHTTKS
                     "
     gene            complement(3574..4434)
                     /locus_tag="ISB17_004570"
     CDS             complement(3574..4434)
                     /locus_tag="ISB17_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP1982424.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1154 a         1086 c         1236 g         1134 t
ORIGIN      
        1 gggatacggg gtgttgtggg ccctcatggt gttgctgatg aatatgcatg acgtgagtga
       61 atcagtggcc ggactggcga ttgcgctgct gacgctgatg gtgtgctcat ctgccggaga
      121 acgtgtgaaa cgcttctggc cgcggcagtt cttcgtgatg ttctatgccg tgcacctggc
      181 ggtactgggt attgtcgtct caatgtgaac gcaggagcag gaaagggtga taccgttcag
      241 gtatagtagc tcttattaac tgaacgatag gcaattgtta tggcagaaga aaaacgccct
      301 gtactgagcc tgaaacgaaa accggcggag aacagcacgg cccccgcaga ggcaacccct
      361 gctcctggtg tggtgcgccg aaagaaggtc gtggtggtct cgtccccgcc ggcctggaag
      421 gcaaagaaag cgaaacttga gaaggctaaa caagcggccg aagccgacgc gcgcaacgct
      481 gcgccggcgc cggataaagc ggtaaagacg ccacctccgg tgcgttatct tcgcctgctg
      541 ccgccggaac aggccatcat gaccctgaag gcattctggc cacagctgtt cgacggaaac
      601 tcaccccgtc tgctggcgag gggaatgcgc gagcagctgt ttgccgacat tgtgaaccgc
      661 gacctgccgc tgtcgcataa acaggtcatt aagtgcctga agagtctgac ccggtctgcg
      721 gactatctgt cccggatgaa ggtgggggcc tcccgctatg atctgcaggg gaatgccgtt
      781 gcgaccgtca ctgcagagga agcgcagtac gccagtgagc gcatgatgaa ggagctgctg
      841 cgagctgaac gaaagatgag tcagaccctt taattatatt tacccattcc tgataattat
      901 acctgggaat cctcagctaa tagatgtaac gaaaattagc tgagggtatg cctgaaatag
      961 caacaggtat tcattttata tttgccgcat tacaggctgc ggcatcataa ctgtccgtgc
     1021 agacagattc tggatgacga tgactgaaaa aattattgct ctcgtagatt atgaaaatat
     1081 tggtacactt gaaaatgtcc gtctgtcccg ttatgaacgc cttattttat ttaccgggcc
     1141 tcagcaggag tttatacgtt ttccagctgt aacgtatgca ggtgatatat cggtaagcgt
     1201 attccaggtt tcgaatgtgt caaaaaataa tgtggacttc catctggtgt ttgagctggg
     1261 aagacttagc gcgactgcac cggaagagac gatatttcat attatctcga atgataaagg
     1321 ctatgatggc gtcattgccg agctatgcag ggcggggaga cgatgttgcc gtatatcatc
     1381 gccttgctct gcagagaaac cgaaggcggc acctatggta atcagtaacg atgaagtgct
     1441 gcacctgacc gataaagtcc agtcattaag taaaaaatct gcagggaacc gcccggcgaa
     1501 tacttcatcc ctgatgaatt atattaaatc cctgtccggt aatacaaaag gcatggcgct
     1561 ttatggtcgc gttaaagagg aattaatcag gcgtggtgtg attgcggtgt atgagaaaac
     1621 agtggtctgg cgataatgaa attaagacgg ctgtgaatgt tatttttatc taaaaaggaa
     1681 tcaatatgtt cagatatttc attctgcgcc ctgagcagca gttattctgt tatttatacg
     1741 gctgcgcgct ggcactggtt cagatggttt tattttctcc tgtatcccgg gcaagcggat
     1801 tttatctggt tgcgttatct gtcgcgctgt tctgggccgg tctggccctg tacacccggc
     1861 acatcgaccg gatgagaaag ccagaggtct caccgctggt cagtattcgc gacggtattc
     1921 aggtggtggc cgaggtgccg cgctatgaaa aggcccggct ggaatgggaa atcctccggg
     1981 atgatgaggt gtttcgccag caaaggtgtg agcttacagg cctgaccgga cgggttatca
     2041 gccgggggct gttgtatacg ccggcggtga tgctggtcgg tatcggtatt ctcgcctggg
     2101 gttccccaca ggatgccatc cggctgatta atgctcttcg taatatgcct gctgcggaac
     2161 tggtgcatca gattggtttt gtcctgtgcc acttcctgca gattagcgtc atatcagttc
     2221 tgattgctga tgttgtagcc gggagaggac tgccgaatgt atttcgccgc gcgttgctcg
     2281 acaggctgcc ggcagagttc tgccttatca gaagagggac agaaagatga ggaagacgac
     2341 aggaaaacgc atcgggaaag aaaggaacat cagggaataa aatacatggc aaagatatta
     2401 atgatggcgg gcagtaccgt tgttgtgctg agtttgctgg gatttctggt gctgcttctg
     2461 aaaggacgcg cagtaacgaa gacttccccg gttctgaggt cactgaaagc cctgggtatt
     2521 cgcccctcag aggctgagca acggttgtgt cgccagcggg tctgggtggg taatgacaca
     2581 ctgatgacgc cacgggaaca acattttttc cgggcattgc tgaggcatac cagccgaaca
     2641 cgctggctgt tgtgtcctca ggtccgggtg gcagatattg ccacactctc tccccatatc
     2701 cggccacgat cccggacctg gtggcaactc ttccggatgg catcacagtg gcactgcgat
     2761 gtggtcatcg ttgacatcca taccttcgct atcatcggcg cagttgaact ggatgatgcc
     2821 tcacaccaga aaaaacaccg tatccggcgc gatattctgc tggaagaggt tctgaggcag
     2881 gccggcattc cactactacg cgatcgggat tcggagagtc tggtaagaaa ggtcgtagag
     2941 tttctgaaat accgggaagc cggtgctgaa gaaaaatcca taaccgttat gaaatcaacg
     3001 acaaagcata gcgaacacaa ggagaaagaa aaatgattat cgccgctgaa gtgaaacgtg
     3061 atgaacaggg gtactggaca catcctgcgc tggtacgaag tggctgtgaa acttccgcag
     3121 aattaagtta ctggctcaga acccataaac tccagtgctt tgtgatgaca atgcgcgatg
     3181 aagcgcctga agctttcgct gccggattta cggatgaagc cccggatgcc cgtggctgga
     3241 taccggagcc accggatgat gatggctggt ttctgggatc tgtccacgat accggcgacg
     3301 gacccgtctg ctactggttt cgtcatacca caaaatcctg acgcctccgc atcgcgtcgt
     3361 tgtccaaccc cgggaacggg gaaaagccag tttccccgct cccggcgttt cttaaacacc
     3421 cgttaagggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     3901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     4201 taccgcacca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4561 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
//
LOCUS       DACADA010000248         4602 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.251, whole genome shotgun sequence.
ACCESSION   DACADA010000248 DACADA010000000
VERSION     DACADA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.251"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            6..572
                     /locus_tag="ISB17_004571"
     CDS             6..572
                     /locus_tag="ISB17_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1982425.1"
                     /translation="MNKKLLSLLVSGSLIAVVSTSVFATDTGVVNFNGKIVADTCEIN
                     VNESASTTGTVTFADTYPADYSGDGSIGTSKDFKIELIKCDPLVDKLNLTFSGTTTDA
                     GFLRLENSEAGAGSATNVGITVTNKNGGANDVKFDGSVPDASTDVDNAGSITPTIFNY
                     TANVIQVGNNAPTAGKYASSATFEVFYR"
     gene            618..1334
                     /locus_tag="ISB17_004572"
     CDS             618..1334
                     /locus_tag="ISB17_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1982426.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKLEENRQTLLHIVFTGDKNKLPQDRE
                     SLFLANIKSVSAMPEELKDRNTLQFAMKARLKLFWRPASLDNSDALTAWEKLKFHKEA
                     GKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPFSEQRFSLPVGAGSI
                     VEWSVITDYGSSSGKRQQKL"
     gene            1401..3878
                     /locus_tag="ISB17_004573"
     CDS             1401..3878
                     /locus_tag="ISB17_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1982427.1"
                     /translation="MLLPLHRTFVLTGITFALSAVYSLSYARDEFNLRILELDSPLEN
                     TQVLEDFVNNNNLTPGVYLTSVMWGQEYLDKRNITFILSSDKKRLIPRFTKADLREFG
                     LKVDDIPALQVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISP
                     KYWSDGESAVWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWE
                     SISTSLQRDIKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVR
                     GIANSDAQVVIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQ
                     ASSTVPILQREGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGAS
                     IYHSGVTGIGADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVT
                     LASYRYSTSQFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYL
                     NGYQQDYWGMHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSR
                     WLPDSWAMYNVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRY
                     RSSVGEFGLGYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKV
                     QNAQGVYTDYWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDF
                     DARSGIRALLTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKE
                     INQQCTGVVTVPEKYTTGIYKATMDCR"
     gene            3900..>4602
                     /locus_tag="ISB17_004574"
     CDS             3900..>4602
                     /locus_tag="ISB17_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1982428.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFK"
BASE COUNT         1354 a          860 c         1088 g         1300 t
ORIGIN      
        1 tttttatgaa taaaaaattg ctttctctgt tagtgtcagg aagtctcatc gctgtagtaa
       61 gtaccagtgt atttgctaca gataccggag tggttaactt taacggtaaa attgttgccg
      121 atacttgcga aattaatgta aacgagtctg ccagtacaac agggacagtc acttttgcag
      181 atacttaccc agcggactat tctggagacg gtagtattgg aacgtcaaaa gattttaaaa
      241 ttgaattaat aaaatgcgat ccgttggtgg acaaactaaa cctgacattt tctggaacga
      301 caacggatgc aggtttcctg cgtctggaaa atagcgaagc tggggctggc agtgcaacaa
      361 atgtgggtat tacggtgaca aataaaaatg gcggtgctaa cgatgtgaaa tttgatggtt
      421 cagttcccga tgcatccact gatgttgata atgccgggag cattactcca acaatattca
      481 attacaccgc caatgttatt caggttggta ataacgcgcc gactgcgggt aagtatgcct
      541 catctgcgac gttcgaagta ttttatcgct aatattcata gtgtttgaaa ggactgtttg
      601 accacggagg gttatttatg tcagtttttt ctcgagtttg tttaggtttt acgtttcttc
      661 ttacggcatt gcaagttcag gcaagtattg ttcttggcgg tacacgtatt atctatcctt
      721 caaatcaaaa tgaagttcaa atcacattaa aaaataaaga tgcatatgca cgctatttgg
      781 tgcaaagctg ggtgagtaat attgatggct ctaaagcccc ctttctcatt acgcctccag
      841 tttataaact tgaagaaaat cggcaaacat tactccatat tgtatttaca ggtgataaaa
      901 acaaacttcc tcaggatagg gaaagtttgt ttctggcaaa cataaaatct gtttctgcga
      961 tgccagaaga actaaaagat agaaatacct tgcaatttgc aatgaaagcc cggcttaaat
     1021 tattttggcg gccagcttct ttggataata gtgatgctct cactgcatgg gaaaaactta
     1081 agttccataa agaagcgggg aagctcatag taaaaaatcc aacgcctttt tatatttcat
     1141 tcagtgatct gacagtatcg ggaaaaaata ttgttcccac cgagagcaaa tctgaacctg
     1201 gcgcattatt gatgatggta ggaccatttt ctgagcagcg gtttagtctt cctgtcggag
     1261 cgggaagtat tgttgaatgg tcggtgataa ctgactacgg ttcaagttct gggaaacgac
     1321 aacaaaagtt ataaatcacc gaagtgtatt actataaata accgatctgt atttataata
     1381 ttacgggcgc aagggaattt atgctgctac cattgcatcg cacatttgtc ttaactggga
     1441 taacatttgc attatcggca gtgtattcat tgagctatgc acgagatgaa tttaaccttc
     1501 gtatcctgga gctggatagc ccacttgaaa atacacaagt gctggaggat tttgttaata
     1561 ataataactt aactccggga gtatatctta cctctgttat gtgggggcaa gagtatcttg
     1621 ataagagaaa tattaccttc attctgtctt cggataaaaa aaggctcatc cctcgtttta
     1681 caaaggcgga tctccgtgag ttcggtctta aagttgacga cattcccgcg ttacaagtaa
     1741 tggatgatga cactgagttt ggtgatattg ctcagattat tgatggcgca cgttatgatt
     1801 tccagttaga cagccaaacg ctgtgtctgc ggatcccgca aatctatcag aatgcgcggg
     1861 ccgcagggag tatttcaccg aaatactgga gtgatggtga gagtgcggtt tggttaagtt
     1921 attacgccag tggttcccgg caaaacagtg atggcgataa cctgaattca aattggttga
     1981 atttaaattc agggattaat ttaggcgtat ggcggctacg aaataatacc gtttacagtg
     2041 actccagttg ggagtcgatc agcaccagtt tgcaacgcga tatcaaagca ttaagaagcc
     2101 agatggaagt cgggcagacg ttcacaaatg gcgatctttt tgacagtgtg cagatgacag
     2161 gtataaagct tgaaacggac accagtatgc tgcctgacag cgaacaaggc tttgcgccag
     2221 tggttcgtgg tattgccaac agcgatgcac aagttgtgat taaacaaaac ggctatgtta
     2281 tttaccaaac gtgggtatcg gcagggccat ttgaaattaa agacttaagc caggtaacgg
     2341 cgggggccga tctggaagtc accatcaaag aaaccaacgg gcaagaacac agttttattc
     2401 aggccagttc aacggttcca attctacagc gagaaggcgc attaaaatat agtcttgcaa
     2461 ctggaaaata ccgtgacaac gataatcatg ctgagacacc tgtttttggt gttgcaaccg
     2521 cgatatatgg cttgccctat ggaataacga tttatggcgg catacttggt gcctcaattt
     2581 atcattctgg tgtgacaggg attggcgctg atttaggccg tctggggtcg gtatctgttg
     2641 atatcaccgc agcagaaacg aagtttgatg atgggcgtga tgatgctaca ggactgtcat
     2701 ggcgtgcaca gtatgcgaaa gattttccag acactgatac gacagtaact ctggcaagtt
     2761 atcgttattc tacatcacaa ttttacactt tccaggaagc acttgatcaa cgtgatacac
     2821 ctgatgacaa gggtatttac agttatcgac agacaaataa tcggcgtaat cgtttacaaa
     2881 ttaatctttc acaaaatatt ggtcgttggg gctcggtcta ccttaacggt tatcaacagg
     2941 attattgggg aatgcatggg gccgaacgca gtataggaat gggatacagt acgacctgga
     3001 gtaacattaa ttggtctgtt aattacacac taacgaaaac gccaggaatg gctggtgagc
     3061 agcaattttc attaacactc aatattcctc tctctcgctg gctgccagat tcatgggcga
     3121 tgtataacgt aaaccgttcc gataaaagta atacatcaca tcaattggga attggcggta
     3181 ctgctctgca agataacaac ctgagttata atttacagca aagttatact gataataatg
     3241 tgggttatga tgccagcatg aatggacgtt atcgtagctc agtgggggaa ttcggtctgg
     3301 gatatagcta tgataaaaat agtcgtcagt ggaattacag tgcgcaaggt gcggtggttg
     3361 ctcatgctca tggagtaaca ttggggcaat cagtgcagga ttcattcgcg atcgtgcata
     3421 ttaatgaggg tgccaatgta aaagtacaaa atgcgcaagg cgtttatact gattattggg
     3481 gaaacgcgat tgttcccaat atgacgaatt atcgacataa cgctattact gtgaatacac
     3541 aggggcacga tagtcttgat atttctgatg ccacacaaga tgttattcct tcgaaagggg
     3601 ctgttgttgg tgtcgatttt gatgcacgta gcggaatacg agccttgttg acattagtcc
     3661 ataataaaga acgcgtaccc tttggggcat tgctgacatt agggaatagt acagcgattg
     3721 ttggtgaaga tggggaggtt tatattactg gtgtgcaaga atcaatgaca tttacggtac
     3781 agtggggaaa agagattaac cagcaatgta caggagttgt cacagtgcca gaaaaataca
     3841 ccacgggtat ctataaagcc acaatggatt gccgataaca aataagacag gaaaaaataa
     3901 tgaagccagg attcgtctct tatatacgaa cattattgac cataatattt cttagctggg
     3961 gcgtcattcc tgaggttttc gccggttgta acatttctgg aaatggagga aatccggcaa
     4021 cagtacaatt ctccgtgcct gcgttggttg ttaatagcga tgccgttcct ggaacgatta
     4081 ttgcgacagt aaatgcgaac agtccgtcaa tttcagtttc ctgtacggca aatggtgaga
     4141 tctggcaagg atataccgta ttgacaaacg gtgatatgcg tactgataac ccacttaaca
     4201 atgtgtttca gactaatgtg ccaggaatcg gttttcgcgc ggcgtgggcg aataacacca
     4261 gtgcgcaact caccgccgga tatttaatga cgccctggca taaaggctcg tcaacagtga
     4321 ttaaaggata tggttatccg attacaatac atgcagctat tcagtttatt gtcacaggac
     4381 ctgtcggtaa tgggaccatt gataccagca aactggttgc tgactggaag tatgacaatc
     4441 gtgttattgg agagcttcgt tttagttctg ttccggtcaa tattcaaccg ggcacatgcg
     4501 accttataga aaaaaatatt accgttccac tcaatgatat tggcacagat gattttgatg
     4561 ttaacaatgt gtcacctgta gtttccgatg atcggtttaa aa
//
LOCUS       DACADA010000249         4461 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.253, whole genome shotgun sequence.
ACCESSION   DACADA010000249 DACADA010000000
VERSION     DACADA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.253"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            10..549
                     /locus_tag="ISB17_004575"
     CDS             10..549
                     /locus_tag="ISB17_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAP1982429.1"
                     /translation="MFATALLSGVAFNALADDPNQGSGKITFKGEVIDAPCSIAPGDE
                     DQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSSASVDATDS
                     NLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNFKARMESLG
                     RTATPGNVQAQANYVLDYK"
     gene            634..3156
                     /locus_tag="ISB17_004576"
     CDS             634..3156
                     /locus_tag="ISB17_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1982430.1"
                     /translation="MNNKNTFTGDTLSHAIKNALSGVVCSLLFVLPAHAVEFNVDMID
                     VEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQALILEWVKADNESGSLLCLTKE
                     DLTSFGLNTEFIESLQTIAGRECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQKQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYADNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVVPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3172..3912
                     /locus_tag="ISB17_004577"
     CDS             3172..3912
                     /locus_tag="ISB17_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1982431.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRGYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVTPFASATQYLNNLPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            3909..>4461
                     /locus_tag="ISB17_004578"
     CDS             3909..>4461
                     /locus_tag="ISB17_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1982432.1"
                     /translation="MITLFRLLAILCLFYNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSDVGIDTFACLDKYEQYYGGIYHDPVCNGSTLQKNVTFNARFRAYV
                     KFKARPAVDQTWDFGVVNVLQFDG"
BASE COUNT         1264 a         1035 c          997 g         1165 t
ORIGIN      
        1 ttgttatcta tgttcgcaac cgcattgtta tctggcgttg cttttaacgc gcttgctgat
       61 gacccaaatc agggttctgg taaaataaca tttaaaggtg aagttatcga tgcgccttgt
      121 tctattgctc ctggtgatga agatcagaca ataaacctcg gtgaagttgc tgatactgta
      181 ttaaaaagcg gacagaaatc tcttcctgta gatgtcacca ttcacttgca ggattgtatt
      241 ttatctgacg gcactaacac tgttgataaa gtcaaaatca cctttagttc tgccagtgtt
      301 gacgctaccg actccaacct gcttaaaaac actctggaag gtaacatcgg cggcgcaact
      361 gatgtaggcg tacgtctggt gaaatcagac aacaccaacg tgactcttgg cactccaatc
      421 actatcaact tcccgacgac taactcctac caggagttga actttaaagc ccgtatggaa
      481 tctctgggac gcaccgcgac cccgggtaac gtgcaggcac aggccaatta cgtactcgac
      541 tacaagtaag cggcaattaa tacgcacaac gtcagacggt aaagccgatg atatgtcatc
      601 ggcaactttg ggtctttttt gaaatggatt tttatgaaca ataaaaacac gtttaccggg
      661 gatacattat cccacgcaat taaaaatgcc ctgtctggcg tagtgtgttc gctactcttc
      721 gttttacctg cccatgcagt ggaattcaac gttgatatga ttgatgttga agaccgtgag
      781 aatatcgaca tctctcgttt tgagaaaaaa ggctatatcc ccccaggcag atacctcgct
      841 cgtgtgcaaa taaataaaaa tatgttgcca caagcgttaa tactggaatg ggtaaaagcc
      901 gataatgaaa gtggttcgtt actctgctta accaaagaag atctgactag tttcggtctt
      961 aatacggaat ttattgaatc attgcaaacc atagctggca gagaatgtct cgatttaagc
     1021 caacgtcagg agttaacgac acggcttgat aaagctacga tgatattatc gctaagtgtt
     1081 ccccaggcgt ggttaaaata ccaggcaaca aactggacgc caccagagtt ttgggatacc
     1141 ggtatcgccg ggtttatcct tgattacaat gtgtacgcca gccaatatgc cccacatcat
     1201 ggagacagca cccaaaacgt cagctcctat ggtacgttag gctttaacct cggcgcatgg
     1261 cgcttacgta gcgattacca atataatcag aattttgctg atggacgctc ggtaaaccgc
     1321 gacagcgaat ttgcgcgaac ttatctgttt cgccctatcc cctcctggtc gtcaaaattc
     1381 actatgggcc agtacgacct gagctccaat ctttacgata ccttccactt tactggcgca
     1441 tcgctggaaa gtgatgaaag catgctgccg ccagatttac agggttatgc gccacaaatt
     1501 accggcatcg cgcagaccaa cgcgaaagta actgtggcac aaaatggtcg tgtactttat
     1561 caaaccactg tcgcgccagg cccttttact atttctgatt tggggcaatc gtttcagggg
     1621 cagctggatg tcacagtgga agaagaagat ggccgcacca gcaccttcca ggttggatcc
     1681 gcatccattc cctatttaac ccgtaaaggg caagtgcgct ataaaacgtc gctgggaaaa
     1741 ccgacatccg tcgggcataa cgatatcaat aatccctttt tctggacggc ggaagcctcc
     1801 tggggctggt tgaacaatgt gtcgctgtat ggtggcggca tgttcaccgc tgatgattat
     1861 caggctatca ctaccggtat tggctttaat cttaaccaat tcggttcgct ttcttttgat
     1921 gtcaccggag cagacgcgtc tttgcagaaa caaaatagcg acaatctgcg tggttacagc
     1981 tatcgcttca actatgcaaa gcatttcgaa tcgacaggta gtcagattac cttcgcgggt
     2041 tatcgcttct cagataaaga ttacgtgtcg atgagtgagt acctaagctc gcgtaatggc
     2101 gatgagtcaa ttgataatga aaaagagagt tatgtcattt ccttgaacca gtactttgaa
     2161 acactggaat taaactctta tctcaacgtt acacgcaata cttattggga cagcgccagc
     2221 aataccaact actccgtatc tgtaagcaaa aactttgata ttggcgattt caaaggtatc
     2281 tctgcatcgc tggcagtaag tcgaatccgc tgggatgacg acgaagagaa tcaatattac
     2341 ttctctttct ctctaccttt acaacaaaac cgcaacatct cctacagtat gcagcgtacg
     2401 ggaagcagta atacttcgca gatgatttcc tggtacgatt catcagatcg caacaatatc
     2461 tggaatattt cagcgtcggc aacggacgac aatatacgtg atggcgaacc aacactgcgc
     2521 ggcagctacc agcactattc accgtgggga cgtctgaaca ttaacggcag tgtacagccg
     2581 aatcagtaca attctgttac cgcaggctgg tacggttcac ttaccgctac acgtcatggt
     2641 gtcgcccttc acgattatag ctatgctgat aacgcccgca tgatggtcga taccgacggc
     2701 atctccggcg ttgaaatcaa ctctaaccga actgttacca acgggctggg cattgccgtt
     2761 gtaccttcgt tatcgaacta caccacctcc atgttgcggg tgaacaataa cgatctgcca
     2821 gaaggtgtcg atgtcgaaaa ctcggttatt cgtactacgc tcacccaggg tgccatcggc
     2881 tacgcaaaac tgaatgccac caccggatac caaatcgtcg gcgttattcg tcaggaaaat
     2941 ggccgcttcc ctccactagg tgtgaatgtc acggataaag cgacaggtaa agatgtgggc
     3001 ctggtcgcgg aagacggctt cgtttatctc agcggtattc aggaaaacag tattctgcat
     3061 ttaacctggg gtgataatac ctgtgaagtc acgccgccaa accaaagtaa cattagcgaa
     3121 agcgcaataa ttttaccttg taaaacagtc aaataattca ggatgaatat aatgaaatca
     3181 ccattaatca caacaggaat gatttgttta ctggggatct gcaattttgc ccaggcgact
     3241 gtctcccccg acaggacacg tatcattttt aatgcttcga ataaaagcgc aacggtccgt
     3301 ctgactaacc aaagtaaaat cgatccctat ctggctcagt cgtggattga agatgccagc
     3361 ggtaaaaaaa cgcgtggtta catatcaaca ctgccgccga tggaacgtat tgagccggat
     3421 gaacaggtac aaattcgcct gatggcgctg gcctcattaa acaacttacc gcaggacaga
     3481 gaaacgctgt tctactataa cgtgcgggaa attccgcctc gcactaaaga acagaatgtg
     3541 atgcaaatcg ccatgcagag tcgactgaaa ttattctggc gtccgaaagc aatcgaatta
     3601 aaagaagggc agatggtgcc tttacaaaaa gtcacaataa cacggaccgc tgcagggctg
     3661 acactcaata atccaacgcc ttatcacatc accgtcgggt atatcggtac taacggcaag
     3721 acactgatgc ccggtgcaga cagcattatg gtgactccgt ttgcttctgc aactcaatac
     3781 ttgaacaact tgccctcaac tttccagtta ggctttgttg ctgactatgg tggactggag
     3841 atgtttaaag tcgaatgtaa ttccgtccaa tccctctgcc aaagctctcc tgctaagaag
     3901 ggaaaaatat gattacgctt ttccgtttac tggcgattct ttgccttttt tataacgttt
     3961 cagtttatgc tgtaaattgc tatcaggatc aaagaggtgg taatacacaa atacgtggta
     4021 ctttaccttc cttcagaatt ccagaaaacg cccagccggg acaaaaaatt tgggaaagcg
     4081 atgacgttaa tgttacggta tattgtgata acgcgacagg atggagagct gatgatcccg
     4141 cagaaaatat ttatgcctgg attaaaatgc cggctataaa tagtgcagac attttgaata
     4201 acccatacct gacattcggt gtaacctata atggtgtgga ctatgaagat agcgatgtag
     4261 gaattgatac tttcgcctgt ctggataagt atgaacagta ttatggtggt atttatcatg
     4321 atccagtttg taatggcagc accctacaaa aaaatgtcac gtttaatgcc cgttttcgcg
     4381 catatgtgaa atttaaagca cgtccagcag tggatcaaac ctgggatttt ggcgtggtaa
     4441 atgttctgca atttgatggt g
//
LOCUS       DACADA010000250         4449 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.254, whole genome shotgun sequence.
ACCESSION   DACADA010000250 DACADA010000000
VERSION     DACADA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.254"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            51..959
                     /locus_tag="ISB17_004579"
     CDS             51..959
                     /locus_tag="ISB17_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP1982433.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1166..3157)
                     /gene="tkt"
                     /locus_tag="ISB17_004580"
     CDS             complement(1166..3157)
                     /gene="tkt"
                     /locus_tag="ISB17_004580"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1982434.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3435..4193
                     /locus_tag="ISB17_004581"
     CDS             3435..4193
                     /locus_tag="ISB17_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1982435.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
BASE COUNT         1066 a         1173 c         1195 g         1015 t
ORIGIN      
        1 ctcctccccc catgtgacat attcccatac tttactcaag gaagattcat atgaaagcac
       61 ctgtttcaat aacctcattt cgcccacaaa aatctattgc tatcgccatt ggcgtgcttg
      121 ccgtggttgt gctgccattt ctctcgtatt acaccgttaa tgaaggtgaa cgcggcattt
      181 tgctgcgcta cggaaaaatc gtaaaagttg ccgagccggg gctgggtttt aagatccctt
      241 ttatggaaag tgtagagaaa atttccacgc gtaatcaggc tgtggtctat cagggattac
      301 aggcctacag ccgcgaccag caaccggcac aaatgacggt atcagtcagc ttccacatta
      361 aaccttcaga agcaggcgcg gtttatacca cctacaacac cattgaagca ttaaaagatc
      421 gtctgattgt ccgtcagttg ccaactcaac tggagaacgt tttcggccaa tataccgcca
      481 tcagcgcggt acaggatcgt acaaaactgg ttcaggatct gcaaaatgca atgcgtaaag
      541 ccgttgttgg cccggtggtg attgatggtg tccagattga aaatattgat ttttctgacg
      601 cttatgaaaa gtcgattgaa gaccgaatga aagcagaagt cgccattgcc acacgtaagc
      661 aaaatctgga aacagagaaa atccaagcgc aaatagcagt aactcaggcc caggcagaag
      721 cagacagtaa actggcggca gccaaagcag aagctgagac cattcgcgta agaggcgcgg
      781 cagaggctga aactattcgt ttaaaaagtg cggctgaagc agaagctatc cgcctgcgcg
      841 gtgaagctct gcgggagaat cccggacttg ttgccctcac cactgctgag cgttgggacg
      901 gtaaactgcc cgataccatg atccccggca gcacagttcc gtttatctct actaagtaaa
      961 aaaaggccgc tttcgcggcc cttgagactg atagcaaaga caaaactgcc tgatgcgcta
     1021 cgcttatcag gctgcaatat gttgaatttg caaaatcttg taggacggat aaggcgttca
     1081 cgccgcatcc ggcataaaca acgcgcactt tgccaacaat ctgaagggcc gcatttgcgg
     1141 cccttctcac aaagcatctt accgattaca gcagcgcttt tgctttcgca acaacgttgt
     1201 caacggtgaa gccgaactct tcaaacagct gctctgccgg agcagattca ccgaaggtgg
     1261 tcatgccgac gatagcaccg ttcaggccaa catacttgta ccagtagtca gcaatgccag
     1321 cttccaccgc aacacgagcg gtaaccgctt tcggcagtac ggattcacgg taagccgcgt
     1381 cctgcttgtc gaatgcgtcg gtagacggca tggaaaccac gcgcgctttc acgccttcgg
     1441 cagtcaattt ttcgtatgcc gctacagcca gttcaacttc agaaccggtc gcgatgaaga
     1501 tcagttccgg ctgaccagca cagtctttca gcacataacc accgcgcgcg atgtttgcca
     1561 gctgctcttc agtacgttcc tgctgcgcca ggttctgacg ggagaggata agcgcagtcg
     1621 ggccgtcctg acgttcaacg ccgtatttcc acgctaccgc ggattcaacc tggtcacacg
     1681 gacgccatgt agacatgttc ggggtcacgc gcagagaagc gacctgctct accggctggt
     1741 gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt gtaaaccatc acctgacgct
     1801 gtttcatcag cgcagccata cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg
     1861 tggaggtgta cggcaggaaa ccaccgtgca gggagatacc gttggcaatc gcagtcatac
     1921 cgaattcacg tacaccgtag tggatgtagt tacccgcagc atcttcgttg attgctttag
     1981 aaccagacca cagggtcagg ttagacggcg ccaggtctgc agaaccgccg aggaattccg
     2041 gcaacagcgg cccgaacgct tcgatagcat tctgagacgc tttacggctg gcgatcttcg
     2101 ccgggttagc ctgcagttta gcaatgaact ctttcgcttt cgcgtcgaag tcagacggca
     2161 tttcgccttt catacggcgg gtaaattcag cggcttcctg cggataagct ttcgcgtaag
     2221 cagcgaattt ctcgttccat gcggactctt tcgcctggcc tgcttctttc gcatcccact
     2281 gagcatagat ttcagacggg atttcgaacg gcgcgtattt ccagcccagc tgttcgcggg
     2341 tcagggcaat ttcagcgtcg cccagcggcg caccgtggga gtcgtgggta ccggctttgt
     2401 tcggggaacc gaaaccgatg atggttttgc acatcagcag ggacggtttg tcagtcactg
     2461 cgcgcgcttc ttctactgcg cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa
     2521 taacgtgcca gccgtaagct tcgaaacgca ttgcggtgtc gtcggtaaac cagccttcaa
     2581 cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc aatcagttta cccagcttca
     2641 gcgtacccgc cagagagcaa acttcgtggg agatgccttc catcatgcag ccgtcgccca
     2701 tgaaggcgta ggtgtagtgg tcgacgatgt cgtggcccgg acggttaaac tgcgccgcca
     2761 gcgttttttc tgcaatcgcc ataccgactg cgttggcaat accctgaccc agcggaccgg
     2821 tggtggtttc tacacctgcg gtgtaaccca cttccgggtg gcccggagtt ttagagtgca
     2881 gctgacggaa gtttttcagt tcttccatcg gcagatcgta accggtgagg tgcagcaggc
     2941 tgtagatcag catggagccg tggccgttgg acagcacgaa gcggtcacgg tcagcccagg
     3001 acggattctg cgggttgtgt ttcaggaaat cacgccacag gacttcggca atgtcagcca
     3061 tacccatagg ggcacccggg tgaccggatt tggctttctg tactgcgtcc atgctcagcg
     3121 cacgaatagc attggcaagc tctttacgtg aggacatttt gactccagat cggatgatga
     3181 agggcacgcc cttaacgact tgacgacagc gcgttttggg ctacgccgga aaatttgcca
     3241 acaatttacc gcaagccgcg cgtcatgtac atggaacatc cttttgccgc ttcagaaatc
     3301 tctggatcat gctcgcatgt tgcgcaatct actcgccggt ccactgcact tttccttata
     3361 cttcgattga gcgtcaattc acctgcagat ggcgcatttt tagaataatc ctgaccttgt
     3421 gcggaagaga aaacatgaaa attcgcgcct tattggtagc aatgagcgtg gcaacggtac
     3481 tgactggttg ccagaatatg gactccagtg gactgctctc atcaggagcg gaagcttttc
     3541 aggcttacag tttgagtgat gcgcaggtga aaaccctgag cgatcaggca tgtcaggata
     3601 tggacagcaa ggcgacgatt gcgccagcca atagcgaata cgctaaacgt ctgacgacaa
     3661 tttctcgcgc gttaggcgat aacatcaatg gtcagccggt gaattacaaa gtgtatatgg
     3721 cgaaggatgt gaacgccttt gcaatggcta acggctgtat ccgcgtctat agcgggctga
     3781 tggatatgat gacggataac gaagtcgaag cggtgctcgg tcacgaaatg gggcacgtgg
     3841 cattaggcca tgtgaagaaa ggaatgcagg tagcacttgg tacaaacgca attcgcgttg
     3901 cggcggcctc tgcaggcgga attgtcggca gtttgtcaca atcacaacta ggagacctgg
     3961 gcgaaaagct agtcaattcg caattctccc agcgccagga agcagaggcc gatgattact
     4021 cctacgattt gctgcgccaa cgcggcatca gtccggctgg ccttgccacc agctttgaaa
     4081 aactggctaa actggaagaa ggtcgccaaa gttcaatgtt tgacgaccac ccagcatccg
     4141 ccgaacgcgc ccaacatatt cgcgatcgta tgagcgcgga tggagttaag taaggcatgg
     4201 tggtgccaaa cgtaggtcag ataaggcgtt tacgccgcat ctgacatgaa tggcacattt
     4261 gttatcctgt gcgcgttgcc ggatgcgatg ctggcgcatc ttatccagcc tacgggtgcc
     4321 gaacgtaggt cggataaggc gctcacgccg catccgacat taatggcaca tttgttatcc
     4381 tgtgcgcgtt gccggatgcg atgctgacgc atcttatccg gcctacgggt gccgaacgta
     4441 ggtcggata
//
LOCUS       DACADA010000251         4426 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.255, whole genome shotgun sequence.
ACCESSION   DACADA010000251 DACADA010000000
VERSION     DACADA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.255"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..700)
                     /locus_tag="ISB17_004582"
     CDS             complement(<1..700)
                     /locus_tag="ISB17_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1982436.1"
                     /translation="MNNQTIRTSVPALDKTVRVCNYLFSSPGATFSQIQQDLDLPKSS
                     TSSLLNALVEHHLLRQERGRFFLGLKLYEWGNRSLEQFDISKVARPVLEKLRDSTDLI
                     CHLGILDEVAPIYILKLESTQPIGIRTWVGKKLPLHSSGVGKALCAWLPQDVIDTLLP
                     EETLPRFTQTTITRKSDLIQEFARIRERGWAFDNAEDSPGIYCIAAPVFNQTREVIAA
                     ISISGVEIQIPKEKI"
     gene            940..1839
                     /locus_tag="ISB17_004583"
     CDS             940..1839
                     /locus_tag="ISB17_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1982437.1"
                     /translation="MLTAFRGIFPPVPTIVDAQGKLDKAGMARMIDHVLENGANGMLI
                     LGSGGEFSQFSSQQRKEIAAFCLSHVDGKVPVLVGIACAGTAETIDLGHHAQEHGADG
                     VMVVNPYYAKLSDDARFMHYKSIADSLKIPVFLYNFPDLTGQDIGLEVITRLAREVPN
                     IVGIKDTIDNISHTREVINRVHAFRPDFIIFSGYDEYMLDTLLLGGHGGIPATFNFAP
                     NITRGIYQAVMENDLDKARYFQQQLAKLSPLYTLEQPFFGVIKTAIKLSGLDISTAVM
                     APALPLSDDKVARVKAILEHIHQ"
     gene            1856..3373
                     /locus_tag="ISB17_004584"
     CDS             1856..3373
                     /locus_tag="ISB17_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP1982438.1"
                     /translation="MNKTPILKLNNSDDVVIARTELPAGSWLESESMETRDLIPAGHK
                     IALRDIPAGSAVKRYGQIIGFATRDIRQGEHIHVHNLGMGSFERDYAWGIDSHLPEKD
                     VHQDTFMGIRRKDGRVATRNYIGVLSSVNCSATVVRAIEDHFRTRGLSDYPNVDGIVA
                     LPQSFGCAFGSDSEMMKVMRRTMAGYATHANFAGIVIVGLGCESFQIKDMMNAHGLHE
                     SPVFHTMTIQESGGTRNAIEKGISFILDMLPEANNVKREPVPVSEIILALECGGSDSY
                     SGISANPALGYAVDRLVRQGGTAILSETSEIYGAEHLLTRRAISREVGEKLIRRIKWW
                     EEYCKRTGSEMNNNPSAGNKAGGLTTVLEKSLGGIAKAGTTSLMDVYEYAEPVTAKGL
                     VFMDTPGYDPMAVTGQIAGGANLLCFTTGRGSAFGAKPTPCLKLSTNNALWQRQRDDM
                     DLNCGTIVDGEETIEQVGERIYQRIIELASGHRSRSEELGYGSQEFVPWIMNSIM"
     gene            3451..>4426
                     /locus_tag="ISB17_004585"
     CDS             3451..>4426
                     /locus_tag="ISB17_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP1982439.1"
                     /translation="MERKPVLGIFLGEAAGIGPELVAKVVADGTVYKYCRPVLIGDAR
                     VLALGQKIAGVHFQWNVISDPTEADWTTGNVPLLDLKNFNPEKLTMGTIDTDSGYATG
                     ESLVTCMSLLKSGKIDGFVFAPLNKEAFKKGGWDIEDEHYLFAEQLGYLDKPRGLLNV
                     LGDLWVFRVTGHIPFKDIASHITPENVSRSIQLCYDTLRMAAVENPRIAVAALNPHAG
                     DGGTCGKEEIDVLIPVIEGFREKGMDMYGPVPGDTVFIHAFNHEYDAVVTLFHDQGQI
                     ATKLHSFDVGITVAAGLPFAITTPEHGTAFDIAGKGIAKTLATERAIAV"
BASE COUNT         1218 a          938 c         1075 g         1195 t
ORIGIN      
        1 caattttttc tttaggtatc tgaatttcca cgccactaat gctgatagct gcaattactt
       61 cacgagtctg attaaatacc ggagcagcaa tacaataaat tcccggtgaa tcctctgcgt
      121 tatcaaaagc ccatccacgt tcacgaatac gtgcaaattc ctgtattaga tcagatttac
      181 gagtgatagt tgtttgcgta aatcgtggca acgtttcctc aggcaacaat gtgtcaatga
      241 cgtcttgagg taaccacgca cataaagcct taccaactcc agaactatgg agtggcaact
      301 tcttgcctac ccacgttctg atgccaattg gttgtgtact ctcgagtttc aaaatataga
      361 ttggagcgac ttcatcgaga atgcccagat ggcagataag atcagtactg tctctcagtt
      421 tttctagaac cggcctcgct accttactga tgtcaaactg ctccagagat cgattccccc
      481 attcatacaa tttcagccca agaaaaaaac gccctctttc ctgacgtaac agatgatgtt
      541 cgacaagggc attcagtaaa gatgatgtgc tgctttttgg cagatccaga tcctgctgga
      601 tctggctgaa ggtggcgcct ggagagctaa aaaggtaatt gcagactctg acagttttat
      661 cgagtgctgg taccgatgtt ctgatggttt gattattcat aaaaaaatat gattccttac
      721 aatactgaat agtatacccc attccccctt gatgcgcagc ggcgcttagc acacatatta
      781 tacatggcct ccgcgtaccg gcatgcctct ataaacctga cttatttcac acttttcata
      841 tcgcattgta aaaacatttg acatgcaaaa cctgaatttg attcacttta ttcagcataa
      901 agaacttgtg ttccatatac agaacttagt gagagtgata tgttgacagc gtttagaggt
      961 attttcccgc cagttcctac aattgttgac gcacaaggga aactagataa ggctggcatg
     1021 gccaggatga tcgaccatgt tttagaaaat ggtgcaaacg ggatgttgat ccttggcagt
     1081 gggggagaat tctcacaatt ttcttcccag caacgaaaag agatagctgc attttgctta
     1141 agtcatgtag atggcaaagt gcctgttctg gtggggatcg cctgcgcggg aactgcagaa
     1201 actattgatt taggacatca tgcacaggag cacggagctg atggtgtgat ggtggtgaat
     1261 ccgtattatg caaaactcag cgatgatgcc cggtttatgc attacaaatc tattgccgat
     1321 tcacttaaaa ttccagtgtt tctctataac tttcctgatc tgaccggtca ggatattggg
     1381 cttgaggtca ttacccggct ggcccgggaa gttccaaata tcgtgggtat caaggatacc
     1441 atcgataata tcagccatac ccgggaagtc atcaaccgtg tacatgcttt tcgtccggat
     1501 ttcattatat tcagcggata tgacgaatat atgctggata ccctgttact gggcggccat
     1561 ggtggtattc cggcgacatt caattttgct ccaaatatca cacgtggtat ttatcaggcc
     1621 gttatggaaa atgatcttga taaagcgcgt tattttcagc aacaactggc aaaactgtct
     1681 ccgttataca cactggagca gcctttcttt ggcgtgataa aaactgccat caaactgagt
     1741 ggactggaca tatctactgc agttatggca ccggctcttc cgctcagtga tgacaaggtc
     1801 gccagggtga aagccattct ggaacacatt caccagtgat tacgaggatc gtaagatgaa
     1861 taagactccg atactcaaat taaataactc ggatgatgtc gttattgcca ggacagaact
     1921 tcctgccgga agctggctgg aatctgaaag tatggaaaca cgtgacctta ttccggcagg
     1981 gcacaaaatt gctcttcggg atattccggc cggcagtgcc gttaaacgct atggacagat
     2041 aatcggtttt gcgacccgtg acatccgaca gggcgaacat attcatgtgc ataatctcgg
     2101 tatggggagt tttgagcgtg attatgcctg ggggatagac agtcatcttc cggaaaagga
     2161 tgttcatcag gataccttta tggggatccg cagaaaagac ggacgggtgg caacccgtaa
     2221 ttatattggt gtactgagtt ctgtaaactg tagtgcaacc gtagtaaggg caattgaaga
     2281 tcatttccgt acccgtggtc tcagtgatta ccctaatgtg gatggtattg ttgccctccc
     2341 acaaagtttt ggttgtgcct ttggctctga cagcgaaatg atgaaagtca tgcggaggac
     2401 aatggcaggc tatgccacgc acgcgaactt tgccgggatc gtgatcgtgg gactgggatg
     2461 tgaatcattc cagattaagg acatgatgaa tgcccacgga ttacatgaaa gtcctgtatt
     2521 ccatacaatg actattcagg aaagcggcgg aacacgtaac gctatagaaa aaggtatttc
     2581 cttcattctc gacatgctcc cggaagccaa caatgtcaaa cgagaaccag ttccggtatc
     2641 agaaatcatt ctggctcttg agtgtggcgg ctctgatagc tattcaggaa tttccgctaa
     2701 tccggctctc gggtacgcag ttgatcgcct ggttcgccag ggaggaacgg ctattctgtc
     2761 ggaaacatcg gaaatttatg gagcagaaca ccttctgaca cgccgtgcca ttagtcgcga
     2821 agttggcgag aaactaatcc gcagaattaa gtggtgggaa gagtactgta aacgtacagg
     2881 gagtgaaatg aataacaacc cttctgcagg taataaagcg gggggactga ccactgttct
     2941 ggagaaatcg ctgggaggga tagcaaaagc tggtacaacc agcttaatgg atgtgtatga
     3001 atatgctgaa ccggtgacag caaaagggct tgtctttatg gatacccccg gatatgaccc
     3061 gatggcagta accggacaaa tcgccggagg agccaacctg ctgtgtttta ccacgggcag
     3121 gggatctgcc tttggcgcaa aacccactcc ttgtctgaaa ctgtcaacca ataacgcact
     3181 atggcaacgg cagcgcgacg acatggattt aaactgtggc acgattgttg atggcgaaga
     3241 aacaatagaa caggttggag aacgtattta tcagagaatt attgagctgg cttcaggtca
     3301 tcgtagtcga agtgaagagc tgggttatgg gagccaggaa ttcgttcctt ggataatgaa
     3361 ctcaatcatg taataactac gggcataatt tgttttgccc ggaaataagg aaacaaactg
     3421 aatcctgttt ttgataatta gaggtgaata atggaacgta aaccagtact gggcattttt
     3481 ttaggtgaag ctgccggcat tggacctgaa ctggttgcca aagtcgtagc tgatggtact
     3541 gtctataaat attgcagacc tgttctgatt ggcgatgccc gggtacttgc tcttggacaa
     3601 aagattgctg gtgtgcattt tcagtggaat gtcatcagtg atcccactga agctgactgg
     3661 actacgggaa atgtaccatt acttgatttg aaaaatttta atcccgaaaa actaactatg
     3721 ggcaccattg ataccgactc aggttatgca acaggagaat cactggtaac ctgtatgtca
     3781 ttacttaaat caggtaaaat tgacggtttt gtcttcgctc cactcaataa agaagctttc
     3841 aagaaggggg gctgggatat tgaggacgaa cattatctct ttgctgagca gttaggatat
     3901 ttggataaac ctcgtggcct gttgaatgtc cttggtgacc tctgggtttt tcgcgtgacg
     3961 ggacatattc cattcaaaga tattgccagc catattacac cagaaaatgt cagtcgttcg
     4021 attcagttat gctatgacac attacgtatg gcggccgttg aaaatccacg aatagcggtt
     4081 gcagcactta atcctcatgc cggtgatgga ggaacctgtg gtaaggaaga aattgatgtt
     4141 ctcattccgg tcattgaagg cttccgcgag aaagggatgg atatgtatgg cccagtaccg
     4201 ggtgatactg tttttattca tgcatttaat catgaatatg atgctgtcgt taccctgttc
     4261 catgaccagg gacagattgc caccaagctt cattcctttg atgtgggcat aactgtagcc
     4321 gcagggcttc catttgctat tacaacaccg gaacatggca ctgcatttga tattgcaggc
     4381 aagggaatag caaaaactct ggccacagag cgggctattg cagttg
//
LOCUS       DACADA010000252         4421 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.256, whole genome shotgun sequence.
ACCESSION   DACADA010000252 DACADA010000000
VERSION     DACADA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.256"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..653)
                     /locus_tag="ISB17_004586"
     CDS             complement(<1..653)
                     /locus_tag="ISB17_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HAP1982440.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATG"
     gene            complement(669..3149)
                     /locus_tag="ISB17_004587"
     CDS             complement(669..3149)
                     /locus_tag="ISB17_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1982441.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(3165..3839)
                     /locus_tag="ISB17_004588"
     CDS             complement(3165..3839)
                     /locus_tag="ISB17_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1982442.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3920..>4421)
                     /locus_tag="ISB17_004589"
     CDS             complement(3920..>4421)
                     /locus_tag="ISB17_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1982443.1"
                     /translation="FMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVP
                     TTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDT
                     VGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLTDNSVNSGDVLSSLV
                     MRVAQD"
BASE COUNT         1236 a         1068 c          822 g         1295 t
ORIGIN      
        1 ttacctgtgg ctggtaattg tgaaacggga atactctcta acgtaattaa taaatcgtcc
       61 ggtttgcagg ttgtggttat cgggttgtat gacaatgtca gattaaagga atagacacct
      121 gcattttgtg ccaatgcatt tctgtagcta tttacacatg catcccatga cttaccacct
      181 aataaacaca gtagaaattg tactgcggca ctcccacgtg tcgatgatga agcactggat
      241 aatgtcgcaa ctccattaat catcactgaa ccaccgcccc ccgcagaaac attcactttt
      301 gcattactgg ccgcaggtgt caccgttacc gaataaccac tggtgaaact ggtctgatta
      361 tttgacaatg taatgttgtt tttatttgtc cattcaaaat gtaaatttaa tttaatggta
      421 tcgttataaa gtccaacaat aatatccgac gggctcatag gggtgatagt atttgtactc
      481 acgcatgacg aatcattaaa attatgctca tatgtcgcca tttgtgtttg agtaagtgca
      541 aaatccccct gaacaaccaa attgtgctga acagataact cactataaca tgccgcatag
      601 ctcacaggca taaccatcat cattaatata aatagcatta tgcgaatttc catagaacca
      661 cctgactatt actggcaaat ataattttta ctttcatcaa tctctttacc gaaggtgatt
      721 gtgcatgaaa gcccttgttg cttatcaatt gccacattaa ccgatggcgg tacttcattg
      781 gtgcgaataa ataactggct tccctggccg acaacaccaa tattatgacc atggatatca
      841 ttgacttcat aaccaaacgt taatggttgc ccatccgctc ttaatgcttt tataaaccat
      901 ggcttgcgct gatcggtatc aaaattaacc agtacaaccg cgccgcgata aggggcggca
      961 attttccggt tgccacgtaa ttctgcttcg ctatcgcttt gtgacacatc caacatcagg
     1021 taattttccc gataaggtgt cattccgtcg tataccacca ctccattacg gtttgttgtg
     1081 cgatattttt gcccattgac ataagcatct ttaattcctg gcgcattcat cacagcaaac
     1141 gtttcagaaa gacggtttgc cagattaacg ccacccgacc aggcgacaat gccccctgaa
     1201 acactggctc cagcctgtcg ataagcactc gactgactat aactgccatt cactgtcgca
     1261 accggcgcgt tccaggttaa attcgcccca gctgtcgttt catttccctg atactgataa
     1321 ctcaggttga ccccatagtt aaactgatcc cggcttccaa cggttcctga taatcccgta
     1381 ttatttgagg caaccccctg atcatcaaac gtcgttgagt tagacatata tatttgccga
     1441 cgaggcgtcg taacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt
     1501 ttctcttcat gatgattctc gtcataagca tggcttgccg cgagggtata gcttatcctt
     1561 cgcaggttgt tggaataact caactgataa tccttactgc tgccgctacg cccccagtaa
     1621 tctcgccata acgtacttaa ggacacagaa ccccagcctt ctggcaatga ctgactcata
     1681 ttggcagaaa agctattttt acggccaaaa tcgttctggt aataatcggc aatgtcatag
     1741 atatcgtttt catcacggcg ataattatct ttattgtttg cccatacgtg atcattaaat
     1801 gtccgataat cacgcgacga ataacgccag gccgccagac caaaacgtgt cgacgtttgg
     1861 ctcacaaatt tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct
     1921 tgtttactat gcgactttgt ggcatcgacg gaaatggcac caaagcgtgt attccagcca
     1981 gtccccaaag tgaacgcgta ataattattc gcgaccatcg agccaccata cagcgttaat
     2041 aagttattaa aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct
     2101 tcaatatggc tacgacccgc cgcaaaatca tatttcgaca cgccgggttg cagcatattt
     2161 ggtaccgctg cataaggcac cagataggtg gttaccgaac cgtccgcctc tttcacgcta
     2221 acatcaagat ccgctccacc accggccaac tgcaaatctg taatcgcaaa cgggcctgga
     2281 ggaacctctt tctgataaac cacaaaacca ttctgttcaa tagttaccag cgcgttactc
     2341 tgagcaatcc cctgcactcg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata
     2401 tcacgaaaca accgcacacc gctgaagcga acagaatcaa aaatatcgct tgatgtgtac
     2461 atatcaccca cacgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta
     2521 ttgctcttcc acacccctgg attgttattt gttttactga aactggcatc agaatgcagt
     2581 tgccacccca ataaatttaa cccgctgtta aaacgtacat atgtactctt gctattaccg
     2641 gacgctttat aatcgctgta atactgactc atataataag aggtataaaa cgcattaata
     2701 ccccgctccc agttttcagg tggtacatag ccaatttcca gttcttccac ccaggcttgc
     2761 gggacactga aatcgagacg aaaaactccg atatcccagg tatagctccc gccctgaata
     2821 agttgcttaa atgttaaaca ttgcttaccg ctggcaaagt tatcggtatt aatgcctaac
     2881 cgcttgatca tttcccttga taaacatgtt tcttgcgggt tgtctttaac aatgatctca
     2941 tatttcccac gccattgctt attgacataa atatcgatgt catactgccc cggcaagggt
     3001 tgattatcat caagacgaat atttgcaacc tgctggtctt tcattccacc tatcatgaaa
     3061 tgggtatcaa aggtttcttc agctgcataa gcttcaatgc cgatcaataa cgctacgatt
     3121 gctgatgcaa gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta
     3181 atatagttgc catggtcatc gataatggtc agatgccagt tatttgcatt attacttttg
     3241 acattaacac tctgactttc taagggggca atcataatag tttcataatt gactttgaca
     3301 ttattagctt tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaat
     3361 ccattgccac tgcgatttac cagtaacttt ttaaaggtcg ctttatttac cggagcaata
     3421 cccgctggcc ggtaaaacaa cttaattctg ttttgcatcg caaacttcag tgcattctta
     3481 ccttcttgtt ctggactatt tggtggaata tccagaacat tcagataaaa aatactctct
     3541 ttattagtgg gtagtttatt cggcataatt ttaattttta cttgctgccc ggaatttgcc
     3601 cctatttttg ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat
     3661 gacgtatcgc catcatcaat ccacgcctgc atcagcgaag aacgatttcc ctggttcatc
     3721 agctgcacca tcacttcttt gtgctctgcc gggtaaataa tacgcgtccc gtagataaca
     3781 ataccggcat gtgcaggaag gaccatagaa gaaatgagta aagctaataa tcctttcatc
     3841 ttaatgcctc ttaaattaaa aggccgccat ggggatagcg gccatgttaa ttacctgaag
     3901 aatcttgcaa ttttatttat taatcctgcg ccacacgcat aaccagggaa gataacacat
     3961 cacctgaatt cactgagtta tctgtcagtt tcaggtattt tgctttaaac ggaatggcat
     4021 attgaccgtc cgctatttta taactctcga tgttgaaagg tgcagaaaca tccaggacat
     4081 cgctgttatc acctaaatag agataataac ccacggtatc gttgctactg gctatcgcct
     4141 tatcgtttcc ggttatttta tcgttggtaa tcaacgagag ttttacacca gcaacttctt
     4201 tcggacagct aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtcgagt
     4261 atttagcttt accttcaaag acagtggttg gtacagttgg catattaata cttactgaat
     4321 cgccacccgc ttcgaattta cacggagatt ctgcaatatt ccctttaatg gttaatgttc
     4381 cggtatcaac gtctgcggca aataccccag cgctcataaa a
//
LOCUS       DACADA010000253         4396 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.257, whole genome shotgun sequence.
ACCESSION   DACADA010000253 DACADA010000000
VERSION     DACADA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.257"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            46..1269
                     /locus_tag="ISB17_004590"
     CDS             46..1269
                     /locus_tag="ISB17_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1982444.1"
                     /translation="MGTIQKIRTDVNVLYAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1254..1898
                     /locus_tag="ISB17_004591"
     CDS             1254..1898
                     /locus_tag="ISB17_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAP1982445.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
     gene            complement(2269..4353)
                     /locus_tag="ISB17_004592"
     CDS             complement(2269..4353)
                     /locus_tag="ISB17_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1982446.1"
                     /translation="MVSTATATSSETKISNEETLVVTTNRSASNLWESPATIQVIDQQ
                     TLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLILIDGQEVTYQRAG
                     DNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGDKLASGVVKAVYN
                     SATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYRNNSQGVWLGYNS
                     GNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFYDTDVDGDYLKKI
                     HFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQAVTLQSHFSLPA
                     NNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQSTVSLFAQNDWR
                     FADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDHEMVTSTSLRYSG
                     FDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNNFELGARYNGNQW
                     LIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRAKTWGLEISAEYN
                     GWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQANIISDVFIRAA
                     SSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDKRYRTAHETIPAA
                     GFNAAIGFVWNF"
BASE COUNT         1134 a          957 c         1095 g         1210 t
ORIGIN      
        1 gtagttcaga atattactgc caagatagtt tatactagga tatgtatggg aaccatacag
       61 aagataagaa cagatgttaa tgtattatat gcggccattc accgtattga gtggttgttt
      121 gagacatttt catctgtttg tttgtctttt tctggaggaa aagactctac tgtgctgctc
      181 catcttacgg ccgatgtggc tcgcaggaag aaacgtcgtt tctctgtatt attcattgac
      241 tgggaagctc agtaccagtg cacaattgca cacattctga agatgaggga gatgtatcgg
      301 gatgtgacgg agacttttta ctgggtggca ctccccctga ctacggtaaa cggtgtctct
      361 cagtttcagc cggaatggat atgctgggaa ccaggtgtgg agtgggttcg tcagccacca
      421 gatgacgcta ttacagatat gtcgtatttc ccattttatc ggtatgccat gacgtttgaa
      481 gaatttgttc cggcattttc ttcctggttt gccggtaacc ggtgtggagt ggcaatactg
      541 actggtgttc gtgctgatga atcgctcaat cgctttatgg gactggtgtc tcagcgcaaa
      601 ctgagatatg cagatgataa accctggacc acagcgtcac ctgaagggtt ttattacacc
      661 ttgtatccgt tgtatgactg gaaagcccgt gatatatgga tatataacgc cagaacccga
      721 gctatctaca atcccctgta tgacctgatg taccgtgccg gcgtgccgtt acgcaacatg
      781 cgggtctgtg agccttttgg cccggaacag cgtaagggac tgtggcttta ccatgttctg
      841 gagccggaaa cttgggccag gatgtgtgag cgggtgtcgg gtgctgccag cggggcgctt
      901 tatgccaatg agagcggtgc ctattttgcc ctgcgtaagc gtatcacgaa gccacctcat
      961 catacctggc gtagctatgc gatgttcctg ctggatgtga tgccggaaag aacggcagaa
     1021 cattaccgta ataaaattgc tgtctacctg cgctggtatc agacgcgggg cttcccggat
     1081 gacatcccgg atgaacagga gaatgacctg gggagccggg atatcccgtc ttggcgacgt
     1141 atctgtaaga cactcataaa gaatgatttc tggtgccgga ccctctcctt cagtccgaac
     1201 aaaccccggc actatgaacg ttatctgcag cgtatgaaag aaaggaggaa ggaatggggg
     1261 attctgtgac acctgaggtg gaagtattga gcaacatgat tcgacagtac ttcagtcagg
     1321 aacgatctga agaagagacc atacgagccc ttaatcatct gcgcagggtg ttgcatgagg
     1381 tcagcccttt tgcacaggag cctgtggact gtgtgttgtg ggtgaaagcg gatgaggtgg
     1441 tggccaacga ttacaatccc aatgtcatgt cttccagtga aaaaaaactg cttaaacatt
     1501 cactggagca ggatggattc acgcagcctg tggtggtttc ggaagagaag gagcattatc
     1561 tggtcgtgga tggttttcat cgtcagttgc tgggcaggaa agcggatacg agaaaacgtc
     1621 tgaagggttg gctgccagtg acctgtatta atccggaaag aaagggacag gcatcacgta
     1681 ttgccgcaac catacggcat aaccgtgcca ggggaaaaca ccagataacg tcaatgtctg
     1741 acattgtcag ggacctttcg cgtctgggct ggacagatga gcgtattggc acagagttgg
     1801 gcatggacca ggatgaagtg ctgaggctga aacagattag cgggctgaca gagctgtttc
     1861 aggaagagaa tttcagccct tcatggacgg ttcgttgata ttgtaaaata tgatagttgg
     1921 agtaaggggc aatagaagga gaatatttat cccaccttct tccgagtcgg gataaatcaa
     1981 aaggtattat ccattgattc agtatctggg tgagagtaaa taatctaaaa atagtattgc
     2041 gttgtgaact gatatcactt ttgaattaca aaggtatagg ctcagtaacc agctttataa
     2101 gtcattgtcg tagcaatgac tatagagtca tgctggcggg aagaataaat attcaccccc
     2161 agatggaaaa caacgtcgaa aaaccttcaa caaacacaat acgttcgtta gcctgcccgc
     2221 aaagcgggca gatcaggaca aagaattcca taacgattgt ttttgtcatc agaaattcca
     2281 tacaaaacct atagctgcat taaaacctgc tgcaggaata gtttcatgtg ctgtacggta
     2341 gcgtttgtct gtcaggttat tgagtgctag gttaatccgg tactgatcct cgttaccgaa
     2401 ttctgtattt actgcaaagt tgagagtggc ccagcccgga acatttgttt cggtaccgtt
     2461 actgtcatct tttgcactag aggcagcacg aataaaaaca tcagagatta tgttggcctg
     2521 acccatcaca agagtatgtt tcagccctat acgtccgttt atcgctggtt ctcctgtatt
     2581 agttgttttt aatgttgaag tttcatattg ccgacgaatt aaattgccac tgatatatgg
     2641 cgagaaaacc cagccattat attccgcgct tatttccagt ccccatgttt ttgcccgatc
     2701 aatattgtca taataatagt agctactacg ggaggagttg gtgttaccat tgcaaactat
     2761 actgccatca cagatcagac ttgcaatata atctttagct tctgagtagt aaactgcgct
     2821 gtcaatcagc cactgattac cattatatcg tgcacctaat tcaaagttat tggagtgttc
     2881 agccttaaga tcaggatttc cgtatgtgac actgccgccc gcagatgtct gcataaaaag
     2941 ctgggagagt gtgggaaata cgtagccttg cgcgaacgca gcgcgtaact ccaagttatc
     3001 gaaacctgaa tagcgcaggc ttgtagatgt taccatttcg tgatcactcg cagactctct
     3061 ggcaagagag gtatcgctta taatgcctgc ggtatatgat actccgtcac cacgcgtcaa
     3121 ctttgaagaa agccagtatt ggcgaactcc cattgtccat gtccagtgat cggcgaatcg
     3181 ccagtcattt tgtgcgaata gtgagactgt actttgctct gactcataat aggagcgagt
     3241 tcgtgtttcc ttattaatga agccggtcag agatttgctt gaggtcatgc caccggacct
     3301 ttggctgacc ctgtcttgtt tgtactgtgc accggtaaca agttcattat tagcaggcag
     3361 cgaaaagtga ctctgcaatg tgaccgcctg agtgtattgc ttatcatggg tgtcagtctt
     3421 gttatgaacg gtcagagcct gaatcatcgg actgggaaca ggctgtgtcg tttttacttc
     3481 gttggcaaat tggcgctgga tggtctgctc atacgcgtcg aaatgaattt tttttagata
     3541 gtcaccgtcc acgtctgtgt catagaatac cccaactttc tctcgttcaa gtttaggtat
     3601 tttgacacta aatgcctcat agcttccgtc tggatcctca tagtaagttt gcgtcgcgag
     3661 tctgtagcga tcaagcgaga ggccaaaacg atggtttccg gagttataac ccaaccatac
     3721 accctgacta ttgttacgat agttggtatt cggcagacgt ccatccggcg tatcacgatt
     3781 gccctgatca gaataactac cgttgatgcg ataatcaaat ccaccgatgc tcccctggac
     3841 cgcgattgat tcttcccagc ctgctgttgc ggaattataa acagctttca caactccaga
     3901 tgcaagtttg tcacctccct ttttggttat gaagttaaca ataccgccaa ttgcctgtga
     3961 accgtacagt acggaatatg gacctttcac tacctcaaca cgctccagcg cagactcatc
     4021 tatcaacagt cccacaccat aattatctcc ggcgcgctga taagttacct cctgaccatc
     4081 aatgagaatt aaaacacggg aggatgcttc gccacgaatg cggatttgtt tacggcctgc
     4141 caaggagttg tctgttatct ctactccggg gatgtcctgc aaattatcgg ctatggaggc
     4201 attggtggag ttctgcaatg tttgttggtc aataacctgt atagtcgccg ggctttccca
     4261 aaggttgctt gccgaacgat tcgtggtcac gacgagcgtc tcttcgttgc tgatttttgt
     4321 ttctgaagaa gttgcggttg cagtagaaac tatcccagaa atgcataatg acagagtgga
     4381 aaggatataa attcga
//
LOCUS       DACADA010000254         4327 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.258, whole genome shotgun sequence.
ACCESSION   DACADA010000254 DACADA010000000
VERSION     DACADA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.258"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..505
                     /locus_tag="ISB17_004593"
     CDS             <1..505
                     /locus_tag="ISB17_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982447.1"
                     /translation="FVVSPQWHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSML
                     VSITWNFIVGFCVLGTALAIRVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGL
                     GLLMLGVASTAGQLLGSVLIDVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSK
                     TMKKMEV"
     gene            508..1542
                     /locus_tag="ISB17_004594"
     CDS             508..1542
                     /locus_tag="ISB17_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HAP1982448.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(1582..1910)
                     /locus_tag="ISB17_004595"
                     /pseudo
     CDS             complement(1582..1910)
                     /locus_tag="ISB17_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420459.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
     gene            complement(1954..3573)
                     /locus_tag="ISB17_004596"
                     /pseudo
     CDS             complement(1954..3573)
                     /locus_tag="ISB17_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3857..4267)
                     /locus_tag="ISB17_004597"
     CDS             complement(3857..4267)
                     /locus_tag="ISB17_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982449.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPSCGTGVYSREGGLQEGNWKDRN"
BASE COUNT         1040 a         1137 c         1021 g         1129 t
ORIGIN      
        1 ttttgttgtg tcaccgcagt ggcattctac ctctttcatc ttacttgcca ttcttccgtt
       61 cttagctggt ttgcttgcag gatggcagcc cgctggaaat gcaaaagtag ctgaagctac
      121 gggatccatg ctggtttcaa tcacctggaa tttcatcgtt ggattttgtg ttttaggcac
      181 agcgctagct attcgtgtcg cattagggca cgtcactgtt caattaccag atgtctggtg
      241 gatgtatctt ggcggcccgc ttggactaat gtctatcggt ttaatggcca ttttggtcag
      301 aggtctgggg ttactgatgc tgggggttgc ttcaacggcg ggccagttac tgggatcagt
      361 attgattgac gtattaattc catcgctggg caataccgtt tatctggtca cgatcatcgg
      421 aacactgttt gcactggtcg gtgcaattgt taccactatt ccggaataca gaacctcgaa
      481 aacaatgaag aaaatggagg tataatcatg aaagattatc tacaaactgt gacgggcccc
      541 gtcgcccgtg aagatatggg gctaacactt cctcatgaac atctgttcaa cgatctttcc
      601 tctgttgtgg atgctccttg ctatcctttt tcacaacggc ttgttgataa aaaagtcacg
      661 gcagaaatcc agtgggcatt aaaacacgat ccttattgct gcgctgataa tatggaccgc
      721 aaacctattg aagatgtgat cttcgaaatt aataacttta tctcgctcgg tggtcgcacc
      781 attgttgatg ccacagggtc tgagtctatc ggacgagatg cgcaggcatt acgagaagtc
      841 gcattaaaaa caggtctgaa tattgttgct tcctccggtc cctacctgga aaaatttgaa
      901 agccagagaa ttcataaaac ggttgatgaa ctggcggcaa cgatcgataa agaattgaat
      961 caagggattg gcgatacgga tattcgtgcc ggaatgatcg gtgaaatagg tgtctcaccg
     1021 acatttaccg aagccgagca taacagcttg cgggctgcct cgctggcaca gattaacaat
     1081 cctcatgtgg cgatgaatat tcacatgccg ggctggcttc gtcgcggtga tgaagtactc
     1141 gacattgtgt taggtgaaat gggcgtctcg ccaaataaag tctctctcgc acactcggat
     1201 ccgtcaggaa aagacgtggc gtatcagcgg aaaatgcttg ataagggtgt ctggctggaa
     1261 ttcgacatga ttggcctaga cattaccttc ccgaaagagg gaatagcgcc aggggtgcag
     1321 gagactgccg atgccgtcgc tcatctcatt gagttgggat acgccgatca gcttgttctc
     1381 agccacgatg tcttccttaa acaaatgtgg gctaaaaatg gcggtaatgg ctggggattt
     1441 gttccagatg tttttctggc ctatctggcg gagcgcggcg tcgataaaac gatcctcaaa
     1501 aaactctgta tcgataatcc cggacgatta ttgaccgcat aaaaccatta tcccccggca
     1561 cactgcgccg ggggatgtca attaataccc cgccgttaaa tcatccacca agcgcgagtc
     1621 ggatgcgccg tacaactcac catccgaccc aaccataatg ctttgcgtgc tgcccatcgc
     1681 ctcattcagc gccactttct gaccttttgc ttccagcagc ttgagcgtat ccggactaaa
     1741 cccttttcaa cacgcagcta gtccggcaac cactgatggt ggaaacgagg cgcattggtc
     1801 gcttcggcaa cattcattcc acaattgatg ctgttcacca ccatttgcag cacggtagtg
     1861 atgattcagc taccggtaac aaagtgagta cataaaggcc atactgtcac gaacagtgca
     1921 aaagtggctc ttccgcaacc taggtgaaga ttttcatgcc agtggactca ttaccctccg
     1981 cctaagtagt tcaaatccgg ctcgaccata catctggcgt atcagcacct tcaggcgatt
     2041 tacatgacct tcgacaacac cattactcca gcgactgctg attgcctcac atatagccgc
     2101 agcatcagct tccatccccg cagctacgca ccgaagttct gctgagccgc tttcgtgaac
     2161 tcgagtgaac cagctgctta gctgtgattt attttaggtt ttcagaatac ggtagaactc
     2221 gagtaccagt tgctgtgcta ttttcaactc cggttctttt tcacacatca gactaataaa
     2281 tcgggatgca taattttctt cgcccctgat tattctccag ggcatcaacc agcggcttac
     2341 ccgggacact gaaggaagtc ttgcggcagt agtaaccggt gggttccagc ctttacgcca
     2401 tttggcaaca gtatccctga cgatggtttc actgcctgtc cccccctggg ccaccatttc
     2461 ccgccatatc cggctggcat tataattacc actttctcgt tgctctttta accactccct
     2521 ccatgggtcc agaagccctc gctttggagg ccgggttgac atttcaggga atgttcctga
     2581 acgcacccag cgactgactg tcactcgcga caggcctgta atacgggata tttccctgaa
     2641 actacatccc ttgttatgca gggccataac ctctgtccaa tgctgatgcc gttttttgcg
     2701 ggtttgctgt ttaaggcgtt ccggacgacg gagcgatgct acaggcacag atatttctgg
     2761 ctcaggtgat ttcttcagtg ataactctct gacaacaaga cgtatcagag gcatatgtct
     2821 gtacatcatt cgttcaggct catcaccaat actttttagc aggtgccatc gatcggccac
     2881 ctgtctggcc tgaggtgcac cttcacgtgc tgctgtcgca tagactccac tgcgatcacg
     2941 cgagacaacc tgtatttccg gatattttct gaaccaggtc gccagcgtac gctgatcacg
     3001 gccgggaagc aggacgaggg gacggtgagt atcaagattg actattaaca taccgcagca
     3061 gtggccccga tgccacgccc actcatcaat accgacatga ggcgcgcctg actgtttcgt
     3121 ctccggggta ttgataaccc tgcgaagaag agtatcagca ctgcaacgga gacccgctgc
     3181 cgttgcagcc cgttttccag cctcacctcc agctattaat cccagttgac gttgtaaatt
     3241 ttgtaacgcc tgtgaagact gctggtgtga accggcgaag ggagcaagcg actcggcaaa
     3301 aattttacgt taacaagccg ggttacggca gtaccagtgg cggacggaaa atacaagcca
     3361 tagcgtctgc cctgagcagg gtagatgctg tatccggcgc cgacgacaac tatgaacaga
     3421 gtcgctacgc ttaaagcatt caggacattg tgctgttttg cgttttccgt ggagatggag
     3481 ggttataccg tcagagctaa taatttgtcg actgcatcgc catccgcaag gtaactgtag
     3541 aagtgtctta agagagtgca ttgcgttgcc cataacttaa aacagcttag atccttgttc
     3601 gccagaaagt ttcatccatt ttcttccacc caggttgcgg aagaaccact tttcatccgt
     3661 cgttgacaga tgtgaagtat gcgggagaaa aagtgaatat ctaactgtaa taactcagac
     3721 cggtacgagt acccgggacg tctacaatca ttcagggcaa agtgctaaaa tttctctatc
     3781 ccgggatgcc cctaccaacg cagcactccc ggagatatca ttacagctct acaactgatt
     3841 tccgcgactg aaccgctcag ttcctatcct tccagttgcc ttcctgcagc cccccctccc
     3901 tgctgtatac cccggtacca caactcgggc agcacttttc tccttcataa tcttcgtcac
     3961 acatcacaca aaaccacttt ctcaccttca ccgtataatc cggaaccgtc ataccctgca
     4021 gatgcaaacg acgggcctct ttctctgccg ggtcaagcca ggtgcatcgc agcaggattt
     4081 tctcagcgac agtcatctgg tcatgttcaa accacggtgg ttgtccgtca ttgccccgct
     4141 gaagtgttaa cgggcatcca caggacacac agcacaaaac gtcctggcgg ttgtgcttcg
     4201 ctgcttcttt cgccgtaaca aaatggcctt tgctgtcatt cgccatataa cagttcagaa
     4261 ttcgcatatt tcctcctgag ttgtccgggg aatactgaat cctccggtct ggcagtgtgt
     4321 gcagtac
//
LOCUS       DACADA010000255         4303 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.259, whole genome shotgun sequence.
ACCESSION   DACADA010000255 DACADA010000000
VERSION     DACADA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.259"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            98..490
                     /locus_tag="ISB17_004598"
     CDS             98..490
                     /locus_tag="ISB17_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982450.1"
                     /translation="MQRKCLICPPSAIYQPIQKLRVIFPDLLFIRKGQGVTPTAFAMY
                     LYESITQGLESILGALDIEGSYDKQRTITIAPSPSVGALVLPVIYRAIKTHYPQLLLR
                     NPPISGAENQLSQFQTDLIINNMFCTGS"
     gene            722..1687
                     /gene="lipA"
                     /locus_tag="ISB17_004599"
     CDS             722..1687
                     /gene="lipA"
                     /locus_tag="ISB17_004599"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAP1982451.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1787..1990)
                     /gene="tatE"
                     /locus_tag="ISB17_004600"
     CDS             complement(1787..1990)
                     /gene="tatE"
                     /locus_tag="ISB17_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAP1982452.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2119..2907)
                     /locus_tag="ISB17_004601"
     CDS             complement(2119..2907)
                     /locus_tag="ISB17_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAP1982453.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3000..3383
                     /gene="crcB"
                     /locus_tag="ISB17_004602"
     CDS             3000..3383
                     /gene="crcB"
                     /locus_tag="ISB17_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAP1982454.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3437..3646)
                     /gene="cspE"
                     /locus_tag="ISB17_004603"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3437..3646)
                     /gene="cspE"
                     /locus_tag="ISB17_004603"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP1982455.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3821..>4303)
                     /gene="pagp"
                     /locus_tag="ISB17_004604"
     CDS             complement(3821..>4303)
                     /gene="pagp"
                     /locus_tag="ISB17_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HAP1982456.1"
                     /translation="ADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDR
                     YNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHL
                     GLGFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYFAWMRFQF
                     "
BASE COUNT         1110 a         1113 c          974 g         1106 t
ORIGIN      
        1 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta cccttctgac
       61 tattttgaag ttgcatatgt ccataaagga atcgttaatg cagcgaaagt gcttaatctg
      121 ccccccctcg gcaatctatc agcctattca gaaactgcgc gttatattcc ctgacctatt
      181 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgtatc tatatgagtc
      241 tatcactcag ggacttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
      301 gcaacggacg ataactattg cgccctcccc ctcggtcgga gccctggtcc ttcctgtcat
      361 ctatcgggcg attaaaactc actatccaca gcttttactg cgcaacccgc ccatcagcgg
      421 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcaataaca tgttttgcac
      481 ggggtcatag atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag
      541 cgcacaacga taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc
      601 tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat
      661 tacaacaatc tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc
      721 tatgagtaaa cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc
      781 ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg
      841 gatgaaaatc aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg
      901 caaaaatggc ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt
      961 taaccacggc actgcaacgt ttatgatcct cggcgctatt tgtacccgcc gttgcccgtt
     1021 ctgtgacgtt gcccacggtc gcccggtagc tcctgatgcc aatgaaccag tgaaactggc
     1081 gcagaccatt gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga
     1141 tctgcgcgat ggcggtgccc agcactttgc ggattgcatt actgccattc gggaaaaaag
     1201 cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgc ggtcgtatgg atcgtgctct
     1261 ggatattctg actgcaacgc caccagatgt gttcaaccat aacctggaaa acgtaccgcg
     1321 tatttaccgt caggtacggc ctggtgcaga ttacaactgg tcgcttaagc tgctggaacg
     1381 ctttaaagaa gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gactgggtga
     1441 aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgct
     1501 aacgctgggg caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag
     1561 cccggatgag ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc
     1621 atgcggtccg tttgtccgct cttcttacca cgccgatttg caggcgaaag ggatggaagt
     1681 taagtaatcc tgtaataatt acttacatct gtctgataaa aaacccgctt tttgaagcgg
     1741 gtttttgtat cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag
     1801 agagcttttc agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat
     1861 tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact
     1921 tcttagtccc aaacagcaga acgaccagcg ccgcaactac cagcagtttg gtaatactaa
     1981 tctcacccat agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag
     2041 agaatattaa cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa
     2101 gcgccgagta aaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagac
     2161 gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc
     2221 aggcatttct gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgccc
     2281 gatatttttg ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg
     2341 agcggcaagc aacgttgacc agtgatgctc tttgagcggc ccacgaaccc aggcggcagg
     2401 aagtaccagt atttcagctc cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa
     2461 gtcataacag gtcatcagac cgaccttcat cccctccacc tccagtaacg gagctatttc
     2521 attaccagca tcaacatggc gtgattcctg aatggcaaat gcatcataga gatgcagttt
     2581 ggcataacgg gcgacgatgt ttcctgcctg tagcgccacc agcatattcc atgcgcgccc
     2641 cggtgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg
     2701 ccgtaaacga ccgaggaatt cgccttccag cagttgtgct gatttaaccg atagatctgc
     2761 atcatgatca tcacgcgcca gtaatgcttc cggcaggaca aacagcgaaa cctcgttttc
     2821 cgccgcctgc gccatcaacg aggcacaaat ctcaacgttc ttttcccaca cagatgtaac
     2881 agcaaactgt cctgcggcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca
     2941 acggcgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag
     3001 tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgtg gcgagatggc
     3061 tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttgggaacg ctggcagcaa
     3121 acctgattgg ggcattcatc ataggaatgg gattcgcctg gttcagcagg atgacgaaca
     3181 ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct
     3241 caacattttc ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc tgggcgttac
     3301 tgaacgtttt cgtcaacctt ttggggtctt ttgccatgac cgcactggct ttctggctgt
     3361 tttccgcctc aaccgcacac taaagaccaa aaaaaacccg ctgattaagc gggttttgaa
     3421 ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct ttggcaccgt
     3481 tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca ttagtctgga
     3541 ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg aaaccgaatc
     3601 ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa attaccttta
     3661 catgaaaaat agacacaaat gctgtgtcgg ttaccagtac accaattgtg gtacgctttg
     3721 tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt taattatttt
     3781 agtggttgat tttaaagaat ttactaaaac ttcatttgtc tcaaaactga aagcgcatcc
     3841 aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc tgaaacgtcg
     3901 cggggccata ccccacagag gccaatggca gtagaaccgg aagagggatg taattccagt
     3961 tatcgcgcgc cgttacgcca gcggtaaatc ccaggcctaa atgaaaattt tcatccgcca
     4021 gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg ttccacgagt
     4081 ccttaaatgc cattgcatac aggccatgcc agtttccttt ttcatcccaa cgcgactgac
     4141 caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg tcataggcaa
     4201 aacgcgcatg ccaggtgatg gcaggaatat ataaatcata atgttctggc tgttgccagg
     4261 tttgtgcaat attttctcta aacgttgtca tccactcatc agc
//
LOCUS       DACADA010000256         4278 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.260, whole genome shotgun sequence.
ACCESSION   DACADA010000256 DACADA010000000
VERSION     DACADA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.260"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..299)
                     /locus_tag="ISB17_004605"
     CDS             complement(<1..299)
                     /locus_tag="ISB17_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1982457.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(414..489)
                     /locus_tag="ISB17_004606"
     tRNA            complement(414..489)
                     /locus_tag="ISB17_004606"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:ggt)
     gene            complement(496..570)
                     /locus_tag="ISB17_004607"
     tRNA            complement(496..570)
                     /locus_tag="ISB17_004607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Gly,seq:tcc)
     gene            complement(687..771)
                     /locus_tag="ISB17_004608"
     tRNA            complement(687..771)
                     /locus_tag="ISB17_004608"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Tyr,seq:gta)
     gene            complement(780..855)
                     /locus_tag="ISB17_004609"
     tRNA            complement(780..855)
                     /locus_tag="ISB17_004609"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Thr,seq:tgt)
     gene            1217..2167
                     /gene="coaA"
                     /locus_tag="ISB17_004610"
                     /gene_synonym="panK"
     CDS             1217..2167
                     /gene="coaA"
                     /locus_tag="ISB17_004610"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP1982458.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2196..3161)
                     /gene="birA"
                     /locus_tag="ISB17_004611"
     CDS             complement(2196..3161)
                     /gene="birA"
                     /locus_tag="ISB17_004611"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAP1982459.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3158..4186)
                     /gene="murB"
                     /locus_tag="ISB17_004612"
     CDS             complement(3158..4186)
                     /gene="murB"
                     /locus_tag="ISB17_004612"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP1982460.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGRAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1109 a         1053 c          960 g         1156 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcgcgagc agcaccgccg
      181 taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc atggtcaacg
      241 tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc tttagacatc
      301 gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca ggcaacatgc
      361 ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag aagtggtgct
      421 gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta ccaactgagc
      481 tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      541 ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct gattttcatt
      601 ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac ttatcgtctc
      661 gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc
      721 tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac cggacttgat
      781 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
      841 ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt tcatgcaact
      901 aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga agaaacagcc
      961 gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat gtgctcctgc
     1021 taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca acgatagctt
     1081 cctgggagag attttttctt attattcctc cccatctggt gttaccctcc tgcccattaa
     1141 cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata gagctatgac
     1201 cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt acctacagtt
     1261 tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat cggaagatga
     1321 gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg ccgagatcta
     1381 tttacctctg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc gtcaggcagt
     1441 tttggaacag tttcttggta ccaacgggca acgcattcct tacattatca gtattgctgg
     1501 tagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat taagccgttg
     1561 gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc ctaatcaggt
     1621 tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg atatgcatcg
     1681 cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag cccctgttta
     1741 ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc agcctgatat
     1801 tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac acgatccaca
     1861 tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac cggaagactt
     1921 acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt ttacagaccc
     1981 ggattcctat tttcataact acgcgaaatt aactaaagaa gaagctatta agactgccat
     2041 gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac ctactcgcga
     2101 gcgtgccagt ttgatcctga cgaaaagtgc taatcatgca gtagaagagg tcagactacg
     2161 caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact acgcagggat
     2221 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agcaccctgt
     2281 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
     2341 cgattaataa aattatccaa cttttcccag cgcgaaagat aaggtgccaa tccttcttgc
     2401 tcgaagagtt ccagcgccgc gcgtaattca cgtatcagca tggccgcaag actattacga
     2461 tcgagattga tcccggcttc ctgcagcgtg atccaccctt gattaacgac actctcttca
     2521 acacgacgca ttgccatgtt gatccctgcg ccaatgacta tttgcgccgc atcgccggtt
     2581 ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
     2641 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
     2701 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
     2761 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
     2821 gcctgctggt attctgcaac acaggcatcg cccgatttaa gctctccgat acgatcaaga
     2881 aggtactgat tcgtggagtc aataacgggc agcacagtta cactaccgcc atccagctga
     2941 cccaatatct gttcagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
     3001 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
     3061 gcccggctca ttcctaacga ttcacccaac tgctcaccag agtgaaattc gccattcgct
     3121 aacagcgcga ttaatttcag cggaacggta ttatccttca tgaaatcgtc tccactgcgc
     3181 taacttcacc tgatgcacca ataaagcgaa cttcaggctc aagccagaca ttaaattttt
     3241 cacccacttt ttgtcttaca tgaagtgcca gttgcaccac atcttcgctt tttgcattgt
     3301 cttcattaat gaggactaac gcctgttgac ggtgcaccgc agccctaccc atttgcatcc
     3361 cttttagctg gcactgatcg ataagccaac ctgctgccag ttttactgaa ccatccgcct
     3421 ggggataatt tggtgctgtt ggaaattgtg ccagtaatac tttagccgtt tcggcagata
     3481 caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca gggagtttgg
     3541 tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt gtaggatcca
     3601 gacgagttaa gtcaccatac gtcagtacag gttgccactc ttttggcaga cgcaggccta
     3661 cggctacaat agcgaagcga tcctggtatt catgtttaaa aatactgtcg cgatagccaa
     3721 aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca acacaatcaa
     3781 cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg ataggtgatg
     3841 agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct tcctgcaaag
     3901 tgtattttac aaggcgatgc cagttttctc cggctcctac atgtaaatac cacgcatcag
     3961 gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg cgatagtctt
     4021 ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct tctgcggttg
     4081 catgctgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg tgctgagcat
     4141 tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct gctttcctga
     4201 tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat atgacgacag
     4261 gaagagttta tagaaacg
//
LOCUS       DACADA010000257         4267 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.261, whole genome shotgun sequence.
ACCESSION   DACADA010000257 DACADA010000000
VERSION     DACADA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.261"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(11..1408)
                     /gene="ccp"
                     /locus_tag="ISB17_004613"
     CDS             complement(11..1408)
                     /gene="ccp"
                     /locus_tag="ISB17_004613"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAP1982461.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
     gene            1813..3462
                     /gene="treF"
                     /locus_tag="ISB17_004614"
     CDS             1813..3462
                     /gene="treF"
                     /locus_tag="ISB17_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAP1982462.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3513..4115)
                     /locus_tag="ISB17_004615"
     CDS             complement(3513..4115)
                     /locus_tag="ISB17_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1982463.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         1087 a         1109 c          991 g         1080 t
ORIGIN      
        1 atcaaattaa ttattgttta tcctgcatat acggcatgta aaccccgttc agactgtgca
       61 ggaaagctac gatatcatcc acatcctcct gcggcagctc tttgcctacc tgatagcgca
      121 gcatcagttt cactgccccg tccagcgtcg gcacgtcacc gcgatggaag tacggtgccg
      181 ttaaagcaac gttacgtaaa ccgggtactt tctgacgcaa tttatcacgc tcttctttag
      241 taacattcat acgaccaata tccgccgccg taatttcacc aaagttaaag tcttttttca
      301 gccccaacgg ttcaaaggaa cgtccgccga gaataatacc accatgacaa gttgcacatt
      361 tattatcttt aaataattga tagccttttt tctgttgcgc cgtcagagca ttctcatctc
      421 cacgcaacca tttatcaaat ggggaatccg gcgtaattaa tgttttctca aattcagcaa
      481 tggcatcagt aatattttcg ccactgaaac cttgcggata gacttcgagg aactgcgctt
      541 taagctgcgg atctttttcc agcttagcaa taatttcgtc ccaggatttc gacgccattt
      601 caatcgggtt caacggcggt ccaccagcct gatcctgcaa tgttgccgca cgaccatccc
      661 agaactgctc aacgttaaat actgagttaa ataccgtcgg cgcgttaatc ggcccaactg
      721 cgccaccaac accaatcgat gtttttctgc catcgacgcc ccccgcattc aacgcatggc
      781 aatgagcgca tgaaatggtg ctatccgccg ataaacgggg atcgtgatac agcgcaaaac
      841 ccaacgccac tttttgcgca tcggtaggca gtttttgcgg gatgggctgc accggttcat
      901 tgcgatgctc cggagcagta tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc
      961 aggccagtat ttccgcccgc tcttcatcac tcaccttacc cgcccagtgt agcgcggtat
     1021 aacgcgttgg cggcatagtt tcatactgca tcacccattc aatcttattc agatcgcttt
     1081 gcgaaacggg tttattagcc agcagagccg cacgcacggc ctcgaggtta aaagatttat
     1141 atccaagctt tatgtcgtaa tccatcaact gtttcgcgcc gggaatataa taataggcgg
     1201 gtaattctgc cgaaggcgta tggcaataat cgcatccttt ttcgcggaga aagcctaaaa
     1261 ccttattatt ttcactgaca gcagatgcct gaacatcggc ctgtttactg cgtttattat
     1321 cgtggtacca cacataacca gataacccta aatagcaaat cgcgacgcca gccaggccga
     1381 tcgcggtaat acgtgagacc attttcatta ttcttccctc acggtagtga gttattgtga
     1441 cccagaaaat tttccagaat gctgcaaacg tctctgcgag acaaagccat cataaaaaac
     1501 agggagatat gctttttgat aggaatcaat caattacatg aatataaact atcaataaga
     1561 tagccagtgc ctattaccca gatcaggaat ggcatatcat gattgcggcg agagcaggat
     1621 tgaatgttct gaaaatgaaa aacaacacga aatccgtatt tttataaatt ccattgatat
     1681 tagtgcgtta tattttttgg caggttaata aacgtgatcc accgcacgct ttgtcgccca
     1741 ccaggcggag cgaatgacta cccttaaaga aaagcccgat aattagcgac gaatttcgga
     1801 ggtcggatcc ttatgctcaa tcagaaaatt caaaacccta atccagacga actgatgatc
     1861 gaggtcgatc tctgctatga gctggacccg tatgaattaa aactggatga gatgatcgag
     1921 gcagaaccgg aacccgagat gattgaaggg ctgcccgcct ctgatgcgct gacgcctgcc
     1981 gatcgctatc tcgaactgtt cgagcatgtt cagtcggcga aaattttccc cgacagtaaa
     2041 acctttcccg actgcgcacc caaaatggac ccgctggata ttttaatccg ctaccgtaaa
     2101 gtgcgccgtc atcgtgattt tgacttgcgc aagtttgttg aaaaccactt ctggctgccg
     2161 gaggtctact ccagcgagta tgtatcggac ccgcaaaatt ccctgaaaga gcatatcgac
     2221 cagctgtggc cggtgctaac ccgtgaacca caggatcaca ttccgtggtc ttctctgctg
     2281 gcgctgccgc agtcatatat tgtcccgggc ggccgtttta gcgaaaccta ctattgggat
     2341 tcctatttca ccatgctggg gctggcggaa agtggtcggg aagatttgct gaaatgcatg
     2401 gccgataact tcgcctggat gatcgaaaac tacggccaca tccccaacgg caaccgcacc
     2461 tattatttga gccgctcgca accaccggtt tttgcgctga tggtggagtt gtttgaagaa
     2521 gatggtgtac gcggcgcgcg ccgctatctc gaccacctga aaatggaata tgccttctgg
     2581 atggacggtg cagaatcgtt gatccctaat caggcctatc gccatgttgt gcggatgccg
     2641 gacggctcgc tgctcaaccg ttactgggac gatcgcgaca cgccgcgtga cgaatcctgg
     2701 cttgaggacg ttgaaaccgc gaaacattct ggtcgcccgc ctaacgaggt gtaccgcgat
     2761 ttacgtgcgg gggcggcctc cggttgggat tactcttccc gttggctgcg tgatactggt
     2821 cgtctggcga gcattcgtac cacccagttc atccccatcg atctgaatgc cttcctgttt
     2881 aaactggaga gcgccatcgc caacatctcg gcactgaaag gcgagaaaga gacagaagca
     2941 ctgttccgcc agaaggccag tgcccgtcgc gatgcggtaa accgttacct ctgggatgat
     3001 gaaaacggta tctaccgcga ttacgactgg cgacgcgagc aactggcgct gttttccgct
     3061 gccgccattg tgccgctcta tgtcggtatg gcgaaccatg aacaggccga tcgtctggca
     3121 aacgccgtgc gcagccggtt actgacacct ggcgggattc tggcaagcga gtacgaaacc
     3181 ggtgaacagt gggataaacc caacggctgg gcaccgttac agtggatggc aattcaggga
     3241 tttaagatgt acggcgatga ccttctgggt gatgaaatcg cgcgcaactg gctgaagacg
     3301 gtgaatcagt tctatctgga acagcacaaa ctgatcgaga aataccatat tgccgatggt
     3361 gttcctcgcg aaggcggcgg tggcgagtat ccgttgcagg atgggtttgg ctggactaac
     3421 ggtgtggtac gccgtttaat tggtttgtac ggcgaaccat aatattttta cagccagccg
     3481 ctaacttcct gctggctgta aaattatcct cttcaggagg agatatttaa catcattgcc
     3541 gcctgggtgc gatttttgac ttccagacgg cgatacagtg attccagatg cgcttttacc
     3601 gttccggtac tgatattcag cgccctgccg atctctttat ttgattcgcc cgccgctaac
     3661 atggttaaaa tctcccgctg gcgggcgctt aacgatttga gatctttaat gtccttttcc
     3721 ggcgtcgttc gccagtctcc aggcagaaac atcatcccca tcgccgcact atttaccgcc
     3781 aacgcaaatg tctcgacggt tgaatcacga ggcacaatgg ccagcacatt aaaatgaata
     3841 acttcctgta accaccgttt gttgcaatcc gtcgccgtaa ttaacacctt aacctcagga
     3901 aattgcacca cagttttttg cagcaaccag tagcaaaact caccatcctg atcgccatcg
     3961 agcatcacta aggcttcagg gtaactttcc agcttttgcc ataactcgtc tgcctgactg
     4021 gccccctgaa tactcactcc tggaatacga tgctgtaaac tgattttcat tccatgaata
     4081 aatattgact gcctgtcaaa catgactatt tgcataactg aatctccacc tgaatacgtt
     4141 aaaaagactt aagtagtgga agggtattac ccgcgagaaa aaataagaat tcgccatttg
     4201 gcggtggcca ttctacagag atgacacgta gaaaatagtt accgatataa atagttacag
     4261 ctaaacg
//
LOCUS       DACADA010000258         4243 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.262, whole genome shotgun sequence.
ACCESSION   DACADA010000258 DACADA010000000
VERSION     DACADA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.262"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(36..2012)
                     /gene="speA"
                     /locus_tag="ISB17_004616"
     CDS             complement(36..2012)
                     /gene="speA"
                     /locus_tag="ISB17_004616"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAP1982464.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2021..2152)
                     /gene="yqgB"
                     /locus_tag="ISB17_004617"
     CDS             complement(2021..2152)
                     /gene="yqgB"
                     /locus_tag="ISB17_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAP1982465.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2288..2503
                     /locus_tag="ISB17_004618"
     CDS             2288..2503
                     /locus_tag="ISB17_004618"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982466.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            2807..3961
                     /gene="metK"
                     /locus_tag="ISB17_004619"
     CDS             2807..3961
                     /gene="metK"
                     /locus_tag="ISB17_004619"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP1982467.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1072 a         1152 c         1028 g          991 t
ORIGIN      
        1 gtaaataaat cgccccgcca gcggaataat cttaattatt catcttcaag ataggtataa
       61 ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg
      121 gttttcttca cctgatcgcg gaactgagtt aacagcgttt tcggatcgag ctgcacatat
      181 tgcagcatgt ccgccacggt atcgccttcg tcagacagtt ctacctctac gctaccatca
      241 gggaagacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat gttgccaagg
      301 atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc cggatcgtac
      361 tccggcatag gcattgtcgt ggcaataccg tcaccatcaa tatagtggtc gatagcaccg
      421 tctgagtcac aggtaatatc caacagcaca gcgcggcgtt ccggcacttg atccagccct
      481 tccagcggca gaaccgggaa caactggtcg atcccccacg catccggcat cgactggaac
      541 agcgagaagt tgacgtacat tttgtccgcc atacgttcct gcagctcgtc gataatcgga
      601 cgatgagcac ggttttgcgg atccagctgc ttttgcactt catggcacat gctcaaataa
      661 agctgctcag cccatgcgcg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga
      721 atatcgtgca gatccatctg gctgtcgtgt aaccattcac gcagagaacg gcgagttccc
      781 ggttcgtgca tttcctgcca ggtttcccac atgctttgca gggcgcgcgg tgcgtcttct
      841 gcaggcgcgg tcggcaccgt gtattcgtta cgttccacgc cgatgatatt cgacaccagc
      901 acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgtggcaga
      961 ccattttctt cacacgcatc accaatcgcc cagatgatgt tgttggcgta ttcattgagg
     1021 ccgtagttca ccgaacagtc ggactgcgaa cgggtgcctt catagtccac gcccagaccg
     1081 ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagctcaac atagaaacgc
     1141 gcggattcac gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg
     1201 aagtgcagca gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc
     1261 agtacctgag tcgccgccag gccgaacttc gatttttccc cgccggagga ctgccattta
     1321 ccggagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt
     1381 tctgcttcat ccagcacaat ggcaatttct gacatcttct caatgaccag atagaccttg
     1441 tggcccatct tctcgccaat taatgccaga cggatatatt cgcggtcctt ataaccgttg
     1501 cagacgatga cgctacgggt catgccagca tgtgccagca ccgccatcaa ctcagctttg
     1561 gaaccggctt ccaggcccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg
     1621 tgctggttaa ctttgatcgg ataaacgagg aagtaatcgc cgttatagcc gtaggattcc
     1681 cgcgcacgtt tgaacgcggc gttaatagaa cgcaaacggt gctgcaggat ctgcgggaaa
     1741 cagaacagtg caggcagacg ctggccctgt gcttcacgag ttttcactaa ctgcgcgaga
     1801 tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta
     1861 acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct
     1921 tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac
     1981 gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg
     2041 taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat
     2101 tttccagcag tgaatgctga cgctccaact gcgcaaccgg tttctttttc ataacattat
     2161 taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt
     2221 ttacaagagc gcccttgttc agtcctcagt agctgtaacc agctcttgaa tcctgagaag
     2281 cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg
     2341 aatcttcacc agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc
     2401 acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgtttt caacccggct
     2461 ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt
     2521 ataccccaca atggcagaaa attgcaaaag ataaatacgc agaatgccag cattgtcagg
     2581 aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaattg
     2641 ggtgaagaac tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact
     2701 cagactgcca gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc
     2761 acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct
     2821 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga
     2881 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta
     2941 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat
     3001 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtacattccg acatgggctt
     3061 tgacgctaac tcctgtgcgg ttctgagcgc catcggcaaa cagtctcctg acatcaacca
     3121 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg
     3181 ctacgcaacc aatgaaaccg acgtgctgat gccagcgcct atcacctatg cacaccgtct
     3241 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc
     3301 gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtatcg atgctgtcgt
     3361 gctttccact cagcactctg aagaaatcga ccagaaatcg ctgcaagaag cggtaatgga
     3421 agagatcatc aagccgattc tgcccgctga atggctgact tctgccacca aattcttcat
     3481 caacccgacc ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc tgactggtcg
     3541 taaaatcatc gttgatacct acggcggcat ggcacgtcac ggtggtggtg cattctctgg
     3601 taaagatcca tcaaaagtgg accgttccgc agcgtacgca gcacgttatg tcgcgaaaaa
     3661 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt
     3721 ggctgaaccg acctctatca tggtggaaac tttcggtacc gagaaagtgc cttctgaaca
     3781 actgactctg ctggtacgtg agttctttga cctgcgtcca tacggtctga ttcagatgct
     3841 ggatctgctg cacccaatct acaaagaaac cgcagcatac ggtcactttg gtcgcgaaca
     3901 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta
     3961 atttttcttc acctgctttt aaaggccagc ctcgcgctgg cctttttctt ttacctcacc
     4021 gcggtggata ggcgttcatg ccgcatccgg caaaaagctg cccgcacaat aacatcattc
     4081 ttcctgaaca cgttttaccg caaattatca tcgctactga aaccgattac accaaccacg
     4141 ccagccaaat atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc
     4201 ttttcagtaa agtcttaatt gcagataaca gcgtttaatc tat
//
LOCUS       DACADA010000259         4234 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.263, whole genome shotgun sequence.
ACCESSION   DACADA010000259 DACADA010000000
VERSION     DACADA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.263"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            53..847
                     /locus_tag="ISB17_004620"
     CDS             53..847
                     /locus_tag="ISB17_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1982468.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            1001..1762
                     /locus_tag="ISB17_004621"
     CDS             1001..1762
                     /locus_tag="ISB17_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982469.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYLDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDCYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..2880
                     /locus_tag="ISB17_004622"
                     /pseudo
     CDS             1804..2880
                     /locus_tag="ISB17_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2902..4095
                     /locus_tag="ISB17_004623"
     CDS             2902..4095
                     /locus_tag="ISB17_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982470.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1153 a          920 c         1072 g         1089 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggttttgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt caccaggctg gcactggagt ctctcatccc
      121 aacggacagg tatatcgtgc gggtgtacag caacgtcact acagaggttg aattcgtttt
      181 aaagacctcc tgtggctacc tgttagctga tatcccttcg cttccttcgg gggagttggt
      241 tcttctggct tgtctcgccc gtttgcggag tgatgccggg caatggcagg tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgctgcaagt ctgtataacg gcttcccacg
      361 catcggcacc atgctgacag cgagtcgtta tcagaatcaa attatccgct ggttactgcg
      421 tcctttgccc ggcgatatga gcgatttctg gctcctgact gcacgggagt tgagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagaccctg catgcgattg ccacacgggc tttaatgaaa cttggactga gaacactgag
      601 cgatttcagg ctgctctata cgggatgcgg tgacagcaga gtgaaacgga atatacggct
      661 acatgcaaaa tatatcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtatccgga atcctttatc cagagaaagt tgccgttggt gaatcaataa attctgacgc
      781 catttccgga aaagtacttt ccggacaaag aaaaaatata tctaaaaaaa gaccaggatt
      841 gttatgacac agcagcgttg ttactaccac ccactgcggc gcacacggac tcgctacagt
      901 gtaggagcca aaatatttct actgttctgg gctgcaggat tgggaggcat gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga attgtaccag cgcagttcgc agctctatca accggttctg tcggcaatgt
     1081 tacctgcata agaacagaag aggagttacg caataaattg cggcacaggc cacagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatctgga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccaccgg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga atgtcagtag agcgtgtcat
     1321 cagtattgca aatgcctctg gtgggttttc aggactgaaa catgcagaaa atttgccagt
     1381 tatggactgt tactctgtct ttatgaaaga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg ttgcttcttg ccggacacag
     1501 ctgggaatac agtgcgcagt ttctcaaaac ggggataaga cagatatggc ttgccgagca
     1561 gtcgctaaaa aagcgttggg ggataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aattttcacg gtgatggaaa tgcattggaa acaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtgggaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaggc attttaccgc agcgcattag cgacagctat tgttatggct
     1861 ctttctgcac cagcattcgc tgctgataac gcggtatcaa ctacaccggt tacattggat
     1921 aaagataaga caactctgga acaagatgtt gttattagtg gtactacaca gattacagcc
     1981 gcaacaatta atgcgaaaga taaagacctt aatgttactt tgggtggtca tgatattacc
     2041 gccacatcaa cggtagacca aggtttcgtt gaaggtgtaa aagttagcgg tgacaaaaat
     2101 gttgtgatta atgctacagg ctccaccatc acagctcaag gtgaaggtac ctatgtccga
     2161 actgcaatgg tcattgattc aactggcgat gttgttgtta atggcggtaa ctttgttgca
     2221 aaaaatgaaa aaagtagtgc gacaggtata tctctggaag cgaccacggg aaataattta
     2281 acgctcaatg gcacaaccat aaatgctcaa ggtaataaga gttccagcaa cggctctacg
     2341 gcaatttttg ctcaaaaggg taatttgttg cagggttttg acggtgatgc aaccgacaac
     2401 atcacccttg ctgactcaaa tattattaat ggtgggattg aaacaatagt tattgccaag
     2461 gagaataagg gaattcatac agtcaacctg aatattaagg atggctcaat aattggggcg
     2521 gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcgcagg ttcagcaacg
     2581 caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct
     2641 gaaagcgaga attcagccgg taccacaaca aatgtaaata tgaacgttgc ccgctcttac
     2701 tgggaaggta atgcttatac gctcaatagc ggcgataaag cgggtagtga tctggatata
     2761 aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaga tgccagtgta
     2821 tctctgcaaa acgggtctgt ctggaatgtt acgggttcct caactgttga tgctctggca
     2881 gtaaaagaca gtacggttaa tatcacgaag gctacagtca atactggcac gtttgcttct
     2941 caaaacggca ctctgattgt tgatgcctct tctgaaaaca ctctggatat cagcggaaaa
     3001 gcgagcggtg acttgagtgt ttacagtgcg ggttcattgg atcttatcaa tgaacaaacg
     3061 gcatttattt ctaccggcaa agacagcact ctaaaagcca caggcacaac ggaaggtggt
     3121 ctgtatcaat atgacctgac acagggagct gatggtaact tttatttcgt aaaaaacacg
     3181 cataaagcat ccaacgccag ctccgtgatt caggcaatgg cagctgctcc ggctaacgtc
     3241 gctaatctgc aggctgacac gctctccgcc cgtcaggatg ctgtccgtct gagcgaaaat
     3301 gacaagggtg gcgtatggat tcagtacttt ggcggtaaac agaaacatac caccgcggga
     3361 aatgcatcct atgacctgga tgtaaatggt gtaatgctgg gtggtgatac ccgcttcatg
     3421 actgaagatg gtagctggct ggccggtgtg gcgatgtctt ctgcgaaagg tgacatgact
     3481 accatgcaga gcaaaggtga cactgaaggt tacagcttcc acgcttacct gagccgccag
     3541 tataacaacg gtatcttcat tgatactgct gcacagtttg gtcactacag caacacggca
     3601 gatgttcgcc tgatgaatgg tggcggtacc atcaaagctg actttaacac caatggtttt
     3661 ggtgcgatgg ttaaaggcgg ttacacatgg aaagacggta atggcctgtt tattcagcca
     3721 tatgccaaac tgtctgctct gactctggaa ggtgtggatt atcaactcaa cggcgtggac
     3781 gttcattctg acagctataa ctctgtgctg ggtgaggccg gtacgcgcgt gggttatgac
     3841 ttcgctgtgg gcaacgcgac cgttaaacct tatctgaatc tggccgcact gaacgaattc
     3901 tctgatggca acaaagtccg tctgggtgat gagtctgtca atgccagcat tgacggtgca
     3961 gcattccgcg tgggtgcagg tgtacaggct gatatcacca aaaacatggg agcatatgca
     4021 agccttgact acaccaaagg tgacgacatt gagaacccgc tacagggtgt agttggtatc
     4081 aatgtgacct ggtaagaata aagcagtcgg ttcccctgtc gactgttata ccccctgcag
     4141 gcagcagtat gctgcctgca ggctataaat atccagtact catgatgctg gctttttgct
     4201 gatatttacc ctgaacattc ctgatacaga ctat
//
LOCUS       DACADA010000260         4093 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.265, whole genome shotgun sequence.
ACCESSION   DACADA010000260 DACADA010000000
VERSION     DACADA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.265"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1764
                     /locus_tag="ISB17_004624"
     CDS             <1..1764
                     /locus_tag="ISB17_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HAP1982471.1"
                     /translation="LTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            1757..3367
                     /gene="tssA"
                     /locus_tag="ISB17_004625"
     CDS             1757..3367
                     /gene="tssA"
                     /locus_tag="ISB17_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HAP1982472.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            3373..>4093
                     /locus_tag="ISB17_004626"
     CDS             3373..>4093
                     /locus_tag="ISB17_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HAP1982473.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPL"
BASE COUNT          973 a         1095 c         1270 g          755 t
ORIGIN      
        1 ctgaccttct ggatggcgaa cctgccggaa cacccgcagt ggaaaacctc accgccaccg
       61 gaactgaccg gcgcggtgcg taaaatcctg ctgcgccaga ttggtgtgcg taatgccgaa
      121 aacaccctct accagaacgt gctgcaacag gtgtcccgca actacgccga tatgacgctg
      181 gcggacatga ccggggatac cctcactgaa tctcttttca gtacggaaca gacggtgccg
      241 gggatgttca cccgtcaggc atgggaagga caggtcaggg aagctatcga gcaggtggtg
      301 acggcgcggc gcgaggaaat cgactgggta ctcagcgacc gccagcagga tacctctgct
      361 gatatctcgc cggatacgct gcgtaaccgc ctcacctcac gctactttac cgactttgcc
      421 ggaagctggc tggcgtttct caacagcatt cgctggaaaa aggaagactc gctctccggc
      481 attctcgacc agctgacgct gatggccgat gcccgtcagt cgccactgat tgcactgaca
      541 gacactattg cgtggcaggt ggcaacaggc agggaaaatc gtggcctgtc agactcgctg
      601 gcgaaatcgg cacaggaact gtttaacggc aaggagaaaa cgccgcagca atcccgtgaa
      661 ggcgacgtgc ctgccgggcc gctggataaa accttcgcgc cgctgctgcg gttgctgggc
      721 gataagtcag gaggcggtgg tggtgatacg caactcaacc tgcaaacgta tctgacccgc
      781 gtcacccgcg tacgcctcaa actgcaacag gtgaccaacg cccccgaccc gcaggagatg
      841 acccagcaac tggcgcagac ggttttacag ggcaaaaccg ttgacctcac cgacacccgc
      901 gactacgggc ggttaatcgc cgccagtctg ggcgaagaat ggagtggctt cggtcaggcg
      961 ctgttcgtcc gcccggtgga acagtcgtgg cggcaggtgc tgacgcccgc agcggacagc
     1021 ctgaaccgcc agtggcaacg ggcgattgtc agtcactgga atcaggactt cgctggccgc
     1081 tatccgttca aagcctcaca gaacgatgcc tctcttccct tgctggcgca gtacctgcgc
     1141 gatgacggac gcatcaacca gtttatcgcc gccaaccttt ccggtgttct gaaacgtgaa
     1201 ggccgctact gggtggctga cgccatgaac acgcaggggc tgacggtcaa cccggacttt
     1261 atccgcgccc tgaaccgcct gcgcgatgtg gccgataccg cctttgccag cggcgatgcc
     1321 ggaatacatt tcgaactgcg ggcaaaaccg gcgcgtgacg tgatgaaaac gcatctggtg
     1381 attgacgggc aggaactgga gtatttcaac cagaaagaac gctggcagcg ttttaactgg
     1441 ccggatgaac agtggcaacc cggcgcatcg ctgagctgga ccagcacaca ggcgatggaa
     1501 cgcatactgg cggattaccg gggaagctgg agccttattc gcctgctgga acgggcgcag
     1561 gtaacaccgg tcgacagcag cacctttaaa gtggtgtgga aagcgcagga cggcctgcca
     1621 ctgaattacc tgctgcgggt tgaacagggt aaagggccgc tggcgctgct ggaactgaaa
     1681 aacttccgcc tgccgggaca ggtatttctg accggcagga gtatgaagga tgcggaagag
     1741 tatggggaag acgccgatga gtgaactgtt gcaaaagctg acccgtagct gttttgccga
     1801 ccgggatgca ctggatgtgg cgcgaacaca ggcggcgctg tggcagacat ggctgctgcc
     1861 ggtgacggca gacacgccgg tcggggaaga ccccggctac cacgacgatt tcctgcgtat
     1921 ccgcgacgaa atgaacaaac tgtccggtgc cgataccggc cttatctgcc agcttgccga
     1981 atccctgctg ctgacgcagg ccaaagacgt gcgcatcgcc acttactaca tctgggcgcg
     2041 actgcaccgc gacggtgagc gcggactggc tgaaggactg gcgctgttag ccgggctggt
     2101 ggaacgcttc ggtacgcaac tgctgccctc gcgaccggca agccgaaaaa tggcgctgga
     2161 gtggctggcg ggggaaaaga tgctcgacag ccttgcccgc tacccggagg tggcaaaaga
     2221 ggattttgcg aacattgttg ccgctcttaa tcaactgtcg gtttccttca ctgcatggcc
     2281 cgaagaccag cattccccct ccctgatgcc gctgattaac gcgctggaat cacgtctggc
     2341 gcagtcgggc gggatgaacg cggtggtgcc gcaaaacagc agcggcgttc cggcaccgtc
     2401 ttccccggtg gatgcgccgc aggtacagac catcacctcc gggcgcgacc tactcgacca
     2461 ggcgaaagtg ctggcccgtt atctgaacga acagccgcag ggctggcttt cagcacaccg
     2521 gctgatgaaa accctgcgct gggacacggt gcatgaactg ccgccggacg tggacggcaa
     2581 aacccggctg gccccgccgc gcaccgaatc acgcaaccag cttaagcggc tgtatgccca
     2641 gcagaactgg acggaactgc tggagcaggc cgacctgatg ttcagcaccg gtgtcagcca
     2701 tttctggctg gatatccagt ggtatctgca tcaggcgctg gcgaaggcgg gcgcaccgtg
     2761 ggacagatgg acagcggtta tccgtcagga cctggcgctg ctgctggagc gcctgccggg
     2821 gctggaaaat ctggcatgga acgacggcac gccgtttgcc gatgaggtga cgcgcaactg
     2881 gatagcgcag caggtcatga tgcgggaaga cggcgcatgg cttgcgggga aagccgctgt
     2941 gccgacagac gacgccacaa atgacgtact ggcgctggag ccggaagcac tggagatggc
     3001 tgacagtcag ggcgtggaag cggcgctggg ctggatacag acccgtccgg gcatcaccac
     3061 ggcacgacag cgcctgttat tgcgactgct gatggcacgg gtggcggaac agtacggtaa
     3121 aaacgagatg gcgctcctgc tgctggaaga actggatacc gccgcacagg gaataacgct
     3181 gacacagtgg gagccagagc tgctgtttga agtaaaagcc cgacaactga aactgctgcg
     3241 tctgcgggcg caccgctatg cggataaagc cctgctgaac cgaaaaatgg aaatcctgct
     3301 gggaacgctg gtggcaatcg atccggtacg ggcagcggta ttgtgtgata cgcaacataa
     3361 ggactgatgg caatgggaac aggctacttt ctggtcaggg gggataagac cacctgtggt
     3421 ggaaaaatta ttgaaggggc ggacgaccat acgatcatgg gtatcccgca ggcacgggat
     3481 atggaccggg tcacctgcgg cagatatccg ggaatgttta ttattgtggg cggcgttcct
     3541 gaaacagata ttcacggcag gctgatggcg gggacgctgg acagtcagag cagttgcccg
     3601 tgtaaagcac ggtttattgc gtcaatgatg gatgatacgt atgagacgga tgacggggaa
     3661 agtgaagcgg aacaacatgc gcagtcggcg aaaaaggatc tgacttctgg ttctgattca
     3721 tcctctgatg atgtaaaact ggattacaga attaaattat caggtaataa gatactgacg
     3781 ccactgaata tacctgatta taaagaaatg ataaacggtg gtagcacgaa gaatacagaa
     3841 aaaatagatt ttaccatcac aaataaaggc gatgaggctg aaggactttc cctggaagtg
     3901 cttgatggca atgaagtgat ttatagtgaa cggcagaccg gtaaatattt tactaaggga
     3961 gagcatgcct ggcaatggga tggctacagt aatcaaggca tactggatac aactaagcta
     4021 aaaagtaaat cactactcgt aagactgatt gctttatgtg gtgatatgat gataaaagtt
     4081 gattatccat tga
//
LOCUS       DACADA010000261         3991 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.266, whole genome shotgun sequence.
ACCESSION   DACADA010000261 DACADA010000000
VERSION     DACADA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.266"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(400..654)
                     /locus_tag="ISB17_004627"
     CDS             complement(400..654)
                     /locus_tag="ISB17_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAP1982474.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
     gene            833..2980
                     /locus_tag="ISB17_004628"
     CDS             833..2980
                     /locus_tag="ISB17_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP1982475.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            complement(3238..3939)
                     /locus_tag="ISB17_004629"
     CDS             complement(3238..3939)
                     /locus_tag="ISB17_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP1982476.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
BASE COUNT          996 a         1041 c         1018 g          936 t
ORIGIN      
        1 ccacctttca cccccaaaag ctccaccttt cacccccaaa agctccactt atcttgccag
       61 aacatcttta aaatcatggt gttacaagac ctaaaaaggt tttaaaaaaa aagatctaaa
      121 aataagatct taaaaactta acggcgagcc tgtggataac ttggggtgct gaaaacaaaa
      181 aaccaataaa aacaatgcgt tgcaataaag cagaattgca agtttcgaat acaccccctc
      241 caaccctgtg gaaacccagt attcatgcgg gttcagcgca tatcacctgc cgaaaaaccg
      301 tgcgtatgtg ctcaacctgg cgactgattc acttccgctt cctcgcccac tcgctcgcta
      361 acgctcggtc gctggggctg cggcgagcgg acgatacaat cactgttctg gcagcgcctc
      421 cagctcgtta atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt
      481 caggaactgg agtaaccagg cacgcaactg cacctcctcc ggctttctga cctttgccag
      541 caatgcagca cccacaacaa ccttctgcgc tgtgtccagg ctccggctct tcgccttcag
      601 gcgctgtaat ctcgcctcag catcagcaat cttctgttcg agcgttctgc tcatgatgta
      661 actccgtgcg cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa aaactgagct
      721 ggcataatgc acggcaaatc acgaagtgcg cacttataca atctccactc cgtttcgatt
      781 gtgtgcgccc tgcggggctg aaagaaaact acaaaaaggc attacggcag aaatggcgat
      841 ttatcatctc agcatgaaaa ttatctccag aagcagcggc tacagcgccg tagcttccgc
      901 tgcctatcgt tccggttcac tgatgcttga tgagcgtacc ggactgactc acgactacac
      961 ccgcaaaagc ggtgtggctg aggctgtgat tctgaccccc gccaccgccc ctgcctggtg
     1021 tacgaaccgt gccgaactct ggaatgccgt tgagaaggcc gaacgccgga agaactcaca
     1081 acttgcgcgg gaaattgaac tcgccatccc ccacgaactg ccgcaggatg ccgcacggga
     1141 aaccgttctc gcctttgtca gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc
     1201 gttccatcac atggataaaa caaatcccca tgcgcacatc atgctgacca cgagggctat
     1261 cggacctgct gggttcagcg gaaaggtcag ggactggaac gaccggacac atgccgagac
     1321 gtggcgggca tcatgggctg accatgcaaa cagggcactg gcgaacgccg gttatcagga
     1381 agagatagac catcgttcat acgagcgaca ggggcttgag aaaacgcctg gtattcacct
     1441 cggaaagtct gcctgtgcga tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa
     1501 tcgtctgatt aacaggctta acctggaaat acagatttcc cgcaccgaac tcaggaacgg
     1561 agggctgaca gagcctgccc gccgtgtggc tgaccttctg aatatcgggc ttcctgacaa
     1621 cgcaacttcg tacacactac gtgacattat cgaagcgttg ccgaaggaca gtaactcggc
     1681 atggaaactc accagtaatg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg
     1741 ctgggaagag ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa
     1801 atcatcgccg ttttcgtccg gtttttcccg tattcccctg atgcaatggg cggcaccgga
     1861 ataccgtagt gagcagggaa aaatccagtc tctgagccag caaatggaaa aattacgaca
     1921 gcattaccgg aaggtggaga aacaggacat tcccgcgagt cagatggcat ttgaaaaaca
     1981 gtggcagcag tggggggaat ccggccttgc agctctcaga gcccagttgc ttaaacgtga
     2041 agaggaattg cgccggaagg aacaggaaga tactgaacgt agtcgcgcgg agcagctcag
     2101 gaaaaatgac aatgccgtta tggattcagg ggtgctgaga gcagtcgtta cggggtatgg
     2161 cgaggctccc atgcctggta acggggaaat gacctgttac cttctgcttc acaaccggaa
     2221 cggagaatat acgctctggg ggaacgagct ggagaaatac cgcaccagaa tctttgagag
     2281 tgttgattta atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa tcggccaacg
     2341 tccgccactg caacgtgttt attcctctgc aacctttgag caactgctga cgcaggtttg
     2401 ccagacatgg ccgcaacata cccgcaatct ccgccagccg aagacatggc cggagtcttt
     2461 ctgtcttgga gaagaccgcc agccagccat gccatcacca gcggcaagga aggtggattt
     2521 tatgcaggga cgtctgccac cgacgctgat gccggtcatg agttctgtcg accgggaaac
     2581 ccgccagctt cagcttttgc tggtcatggg cgtggatgac tctctgggcg gtgttatccg
     2641 gcttaatggt acgctgtatc ccgcgtttgc cgttccctca gccgataaca ggcatctggt
     2701 catcagtgcg ctgaccgata aggggttacg gtatgcaggg tatggtgttg ccgtaaatca
     2761 cgatgcagac agccacatat cacctgcacc agaactgatg cagtttcatc tgaaaacccg
     2821 tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa cagccggatc atctgttccg
     2881 ctcgctgggt tttaatcgta cctgggagga atggcagcgc gaggaagatg cacgaacaca
     2941 caccaccgag aggagacatg atcgcggttg gtcacagtga atgtgaacaa tattttgttt
     3001 tgtttaacat cgtgttatgt tcgtcgatgt tcttcgatgt tcatcgacag ggatgaatgc
     3061 aagaagcaca ctcacataac gcaagcatag ttttgttttt ccgcccccaa aaattggggg
     3121 gtcttttttc gctgcatcag agcgtttttg acgcaatttt tcacttcaat agtaccttcg
     3181 cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgatttta
     3241 gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg
     3301 cccctttttg atcgtctcaa cagttacaac cagatcgctt ttttcgttca gttcggcgat
     3361 ggctggcttg attatcatct tattcaagtc tttgaattgt tgatatttat cttcaacggc
     3421 aagcatggtt cggaaatcat cgatagctat cgttctgtca ccggtgctgc ggaactgctg
     3481 gagcagctcg taaatgcgga tgctgtaggt gctggaaagg ctggagacgt tcttcacgac
     3541 tacccgcgta aactgcccct tgagctgtgt cagatacggc atgatggcat cggagaaggt
     3601 gatatctact ttcgcctcac ctttgaaata ctgcgcccgg tactgcaccc agcggaactc
     3661 ttctcgtttt tcctcgttct tgatgattac tgacctgtcc cagaggcgat ctatcgcttc
     3721 cttgagccta gcttcagcgt gctgccgtcc catgtcagga aaggcatcat gaaactctgc
     3781 agccgtgatg gtcatggttt tttgttcatc cggcgaaggg gaatcttctc cggattttag
     3841 gcggctgata cagaccagca ggaagcgctg ttcctgcaaa gtgagcttgt agcttgcctc
     3901 gataagggcg tttgatttgt aagctatcag atctgacatt tcgcttgctc gttgctattg
     3961 gtggcgtttt tttactgtac gccacttatc a
//
LOCUS       DACADA010000262         3987 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.267, whole genome shotgun sequence.
ACCESSION   DACADA010000262 DACADA010000000
VERSION     DACADA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.267"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(98..889)
                     /locus_tag="ISB17_004630"
     CDS             complement(98..889)
                     /locus_tag="ISB17_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HAP1982477.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(896..2866)
                     /locus_tag="ISB17_004631"
     CDS             complement(896..2866)
                     /locus_tag="ISB17_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HAP1982478.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            3103..3587
                     /locus_tag="ISB17_004632"
                     /pseudo
     CDS             3103..3587
                     /locus_tag="ISB17_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1011 a          947 c          920 g         1109 t
ORIGIN      
        1 tgctcccctg gcgcgtagca ctgccgactg aataacacat ccccgtcaat acggttcttg
       61 ctgcacgctt acaatttcag ggcagggaat agtattgtta atttcgggag aaacttaaag
      121 ttgccacatc gacagtttca tcacacaggc ttttgtcctg aaaacttttc ttattttgga
      181 ctttcgccag ccactgtcca atatgaagag aaggtactgt gacgatacca tcctcattag
      241 ttacaaaggt gagttctgct ttatgaacag gatgtttgtg ctcaactgca tgagtatgac
      301 gggatgattt cagataactg accgagtcaa gctccatgat agctccggcg agcggggacg
      361 ttttatacag gactttcagc ttcacatctt cactgaaacg acttatatcg gataacggaa
      421 tgatttcaag ctcatgcccc gttggagact gatagagttg tgctggcatc tgctgttcac
      481 caggaataaa agatttcgca tatttagtga caaactcaca gtaagccaca tcatttctgc
      541 cctcccggct gaccggtttc catcctgaag aggttctcga ccagtatccc ggtttcatat
      601 atgttgacaa aacgtagcca ttatctttgc ttgcagactc agttttatag acataatccg
      661 tggacgctgg cttcagtgtt attgtctctt tcccgttata aatccgggga ttttcaaaga
      721 aattccttct gtcaggaatt gtgccctttg aggggaaatt atgaccataa ccaatctctg
      781 cggtaatgtt gttctctgcc tgtttacctg taatccagac atcgtgggcc aggacatgcc
      841 ccgatgctgt cagacaaaga gctaagatat aacctgtatt tttcatcatg atttgttacc
      901 actgataacg ggtataaaga ctgacagttc tgcctgaaag cggcactcct ttgccattca
      961 tggcaagctt gttgaaggca ttatccagag tcactgatgc ctcaaagcct ttaacactta
     1021 caggcttata agacagtgaa aaggaatgta cggcatagcc agcctgatgg ggatcgcctt
     1081 ttttcacatg cgtggctgct gccgaaagtt cagcacgcca tccggcagaa actcctccat
     1141 gaccaacagg tatacttatg tcactgatga gtgtgtcagg attgccggag cccagccatt
     1201 ccctgctgct ggcatccata ctttccgtgc ggttataaga aagcatccag tcaaaataat
     1261 cagactggta atttccctgc atatcccatc cccagatacg ggcccgggat acattataag
     1321 aataagaacg catccgtttc atatccacgc gcgtggcaat atagtttctg gcatccgttc
     1381 cgaaatatcc gcctttcaac tgcagccgat cattgcctgt aagcaggccg tccagttgaa
     1441 tacctgcgcc aatctcacgg gtaatgttat tttctggttt cagattaagg ttaggtaccc
     1501 agtaattggg tttacccttg cggtaaaaat gtacatcatc cctgtacatt tctgccattg
     1561 tgggggcacg gaatgcagag gatatggagc cgtacatcat cagccagtct gtcggtgaga
     1621 ctgaaatcgc cgcccttggg gacaggcgtt cggctttcag ttctccggca cggggattga
     1681 agctcttgta ccggtcatag cgggaaccga cgataatgtt aaccgggtag cttttcattg
     1741 tcatttcatc ctggaaatac aacgatgtaa agtcaatgtt tccttccgga tatagtgtgg
     1801 cagagccttc tggcttctgc tgctgacggt aatattcagc gccataactt aactgatggg
     1861 ctcccggaaa aatcagagta tgactcctgt tgacaacatt aacaccggag gttctgttgt
     1921 ttctccattc tgccgttttg ttgtgaccgt ttgtcttgat agtgatatca ctgagatata
     1981 aagttgactt tacattgatg aggctgttat ccacaggagc gtactggtac cagaactgta
     2041 cgtcattttg tactgttttt ctgtcacgca ggccgctgtc accatgtgtc tgagtgggat
     2101 tgcccggttc agtggtgtta ttccggtaga gacgcaagct ggcacctgct ttgttactgt
     2161 ctgttatacc gacagagcct ttcgcaaaca gggctgcagg tttttccttg ttaggtgcgc
     2221 gctcaccatc actttgatag atattacctc ttttacgcat aatgacagaa aggagcgcat
     2281 ctgtttttcc ggttttaccg aaccaggtga gccccgagcc tgtgctgtgg tcaccactgg
     2341 cgatatttcc ccacagactt aaaccgtttg tctctccggg gggaaggaaa tctgctgcat
     2401 cggcagttct gaagtccaca acacctccca gcccgccact gccgtacaga ctggagtttg
     2461 gtccgcggac aacttcgata cgtttgacga gtgccggatc cagataagtg ccactgcttt
     2521 ttgccatgtc actgagttga cggatgccgt caacaagaac aagtaccccg cttttgtcat
     2581 agccgcgtag attaaaggtc tgcccattgg tccgtcctgc accagtctgg ctgagaccgg
     2641 caacaccttt cagcatgccg gctgatgtga ccgcattctg actccatggc gtgtcattcg
     2701 tgacgacact gaccattgac ggcgtttcaa ataccgtgtt ctgatttcct gtcgcgacaa
     2761 cggtgattgt atctttagcc atagaagaat gactggtctc tgcctgtaac cctatcggga
     2821 gtatgattaa tgtacaaatg acgtttctga ttacgttcat atacatgcga aaatatatgt
     2881 taatattgat atgttataac atatcatttt attaatgcaa ccctgatcac gcagcgttca
     2941 gtgataacca gtgaatagcg actctaatac ccggtcgctc tgcaatgtat cagcgcctgc
     3001 gaccgatgct atggatattg atttttctac cgataaagaa ccagcaatac ggaatttaga
     3061 ctggccccct gaatctccag acaaccaata tcacttaaat aagtgatagt cttaatacta
     3121 gtttttagac tagtcattgg agaacagatg attgatgtct taggaccgga gaaacgcaga
     3181 cggcgtacta cacaggaaaa gatcgctatt gttcagcaga gctttgaacc gggaatgacg
     3241 gtctcccttg ttgcccggca acacggtgtg gcagccagcc agttatttct ctggcgcaag
     3301 caataccagg agggaagtct tactgctgtg gccgcaggag aacaggtcgt tcctgcctct
     3361 gaacttgctg ctgccatgaa gcagattaaa gaactccagc gcctgctcgg caaaaaaacg
     3421 atggaaaatg aactccttaa agaagccgtt gaatatgggc gtgcaaaaaa gtggatagcg
     3481 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
     3541 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggaaatat aggggcaaat
     3601 ccacatcctt acttttccgt aaggatttta gcgggtcaaa acagcgttcc atgccggact
     3661 gatgatctac atacttagag tcacatacca aagtaaaact atggatgtat gcaagcctgc
     3721 atcgtcagga ggttaaattt cagacctgcc agttaccgcg taaatggctt gatatatgac
     3781 taatttattt gcacaggatt tttacgacaa cgctctgaac cagcagttga cgggatatat
     3841 agttgagctc attacagata aagcttttct gtatctctgg cggtggtcat agaccagtaa
     3901 tcgttattgg ttgttctata ttatcccgta tgacggtaca acgtttttaa caactggcgc
     3961 tgtcacaaca taagccaccc caaaata
//
LOCUS       DACADA010000263         3956 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.268, whole genome shotgun sequence.
ACCESSION   DACADA010000263 DACADA010000000
VERSION     DACADA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.268"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(51..686)
                     /gene="msrQ"
                     /locus_tag="ISB17_004633"
     CDS             complement(51..686)
                     /gene="msrQ"
                     /locus_tag="ISB17_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAP1982479.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(687..1691)
                     /gene="msrP"
                     /locus_tag="ISB17_004634"
     CDS             complement(687..1691)
                     /gene="msrP"
                     /locus_tag="ISB17_004634"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAP1982480.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1800..2213)
                     /gene="hiuH"
                     /locus_tag="ISB17_004635"
     CDS             complement(1800..2213)
                     /gene="hiuH"
                     /locus_tag="ISB17_004635"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAP1982481.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2346..3017
                     /gene="hprR"
                     /locus_tag="ISB17_004636"
     CDS             2346..3017
                     /gene="hprR"
                     /locus_tag="ISB17_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAP1982482.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3017..>3956
                     /locus_tag="ISB17_004637"
     CDS             3017..>3956
                     /locus_tag="ISB17_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP1982483.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
BASE COUNT         1100 a          849 c          945 g         1062 t
ORIGIN      
        1 ctcgcgccgc atccggccgg gataacgcga agtttgtcgg caatatgaag ttatagagat
       61 aatttgttgt gggcctgctt gcgtaaccgg ttgaaaaggg aaagcaactt cttataccgt
      121 aaggctaaaa gcagtacagc cagcccagcg tagatgagtg gctgcggtga aataatcttc
      181 actgaccaca gataatgtat tggggtcagg atcgcgacaa gatagacgaa gttatgcaac
      241 tgttgccagt gtttgccgag ttttcgctgc atcgactggg ttgaagtgaa cgctaaagca
      301 agcaaaataa cccagctgat aattcctaac gttaaataag gccgggtaat taactcctta
      361 cctaataacg ccagattatt cacgcccaac tccagcaatg cgtaactggt taaatgcagt
      421 gtcgcccagg caaagcacca taagcctaac aggcggcgag tgcgtatcaa taacggctgt
      481 tttgcgtagc gtgccagagg tgtgattaac aaggccgcaa gcaaaaattt cagtgcagtg
      541 cgaccggtaa aatgctgaat atctttcacc ggatcggcac ctagtccacc gtgattgatc
      601 gcccagacca gccagagaaa cggcaacaat ccggcaagat gcaggcaaac tttcagccat
      661 atcacctgtt ttgctgtcag gcgcatttag aaattctccc gcaaatccag gccacgatac
      721 agcgatgcca cctggtcggc gtaaccatta aacagtagtg ttggctggcg ctgaacatcg
      781 agaatgccgc ctgaaccaat aaatcgttcg gtagcctgtg accagcgcgg gtgatcaaca
      841 tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg ctgccagatt ccaggtggtt
      901 ggcggacgct cgcgggtcag cttaatactg acgatcgatt taatcccttt aaagccatat
      961 ttccacggca caatcagtcg caccggcgcg ccattttgcg gtggtaacgc cttgccataa
     1021 acacctacgg tcatcagtgt gagcggatgc attgcttcgt cgagacgcaa tccttcgaca
     1081 taaggatatt tcagcccgcc gccgataaag cggtcctgct ggcctggcat ctgttccggc
     1141 gcataaatcg tttcgaaagc gacatatttc gcattgctgg tgggttcggc aagcgccagc
     1201 aatttgtgta gcggaaaacc aatccacggc accaccatcg accacgcttc cacgcagcgc
     1261 atacgataaa tacgctcttc cagcgggaag cgacgggtta aatcatcatg atcgagggtc
     1321 aatggttttg ccacttcgcc gctgattttc agtgtccatg gatcggtttt caggctacca
     1381 gcattagcag cgggatcggc tttatccagc ccgaattcat agaagttgtt ataaccggag
     1441 actttatctg ctggcgtcag tggcaggtta ttttgccagg cggcaggctt gctgaactcc
     1501 agcggttttc cggcgggggc gggcggacga tcgttccctt taaaccagct aagcagatcg
     1561 gcatgcgcag cgtgaggcaa agaaaatgca gctgcgctga tgcccagtgc ttttaacacc
     1621 tgccgacgct tcataaagaa taccgactcg gccgtaacat ctgattcttt taaaaattga
     1681 ttctttttca taagccatca cacttttttg tcgttagcca acaagagccg tttactgtta
     1741 atctgtcagg gagtcacctc cctaacagta gcagagatca gcttttacat atttacgcat
     1801 taactgccac gataggttga atacccatat tggctgagta acaaaggcac atgataatgc
     1861 tcgttcactt tattaatatg aaattcaacg gggatctcag ggaagaaact ttcaagattt
     1921 tgtttcttga aatagtcccc ggttttaaat acgacacggt aatcgccagt agtcgcagtt
     1981 tgctcgggcc acagtgcctt aattcgtcca tccttatctg ttttggcggt attaagttgt
     2041 aaccagccgt tatccgcttt cttttcaaga gtgactgtca cgtcggcagc gggttttcct
     2101 gtttgctggt tcaaaatgtg cacgctaaga atgttttgtt gtgcggcata aaccaaagaa
     2161 ggtaatgaaa atgctgccgt tgctaccgag agtactaaat aacgctttaa catgtttata
     2221 tccttgtcat gtgaatgagt tttgaggata gcgaaaaaac gtccagggta accttacagc
     2281 agcattacaa ttttgtaatg aaccaggttg tttctataac atatgattta tggcatatta
     2341 ttttcatgaa gattctactt attgaagata atcaaaggac ccaggaatgg gtaacgcagg
     2401 ggctttccga agcgggttat gtcattgatg ccgtttctga tggcagagat gggctttatc
     2461 ttgcgctgaa ggatgattat gcattgatca ttctggatat tatgcttccg ggtatggatg
     2521 gctggcagat cttacaaacg ttaagaacgg caaagcaaac tcctgttatt tgccttacag
     2581 caagggattc tgtcgatgac agagtcagag ggctggacag tggagcaaat gattatctgg
     2641 taaaaccttt ttcattttct gagttgctgg caagggttcg ggcacaatta aggcaacatc
     2701 acgctttgaa ttcaacatta gaaatcagtg gcttaaaaat ggactctgtc agtcaaagtg
     2761 tgagcaggga caatatcagt attacactga cgcgcaagga gtttcagtta ctttggctac
     2821 tggcctccag agctggcgag attataccca gaacggttat tgcgagtgaa atttggggaa
     2881 tcaactttga tagtgatacc aatacggtgg atgtcgccat tcgcaggctc cgcgcaaaag
     2941 ttgatgatcc ttttcctgaa aagctaatcg ccacaatccg ggggatgggc tattcattcg
     3001 tagcggtaaa aaaataatga aaagattatc tataaccgtc cgtttaacct tgctttttat
     3061 atttctgctg tctgttgctg gcgctggaat tgtatggact ctctataatg gcctggcaag
     3121 tgagttgaaa tggcgcgatg atacaacact cattaaccgg acagcgcaga tcaagcagtt
     3181 gttaattgat ggggtaaatc cagatacatt acctgtctat tttaaccgga tgatggatgt
     3241 tagtcaggat atcttgatca ttcatggtga tggcatcaat aaaattgtta accggacaaa
     3301 tgtcagtgat gacatgttaa ataacatacc tgctagtgag acaatcagcg cagcaggcat
     3361 ttacagaagc attattaatg atacagagat agatgcttta cgaattaaca ttgatgaagt
     3421 ttcgccatca ttaacggtta ctgtggctaa attggcttca gccagacata atatgcttga
     3481 acagtataaa attaatagca ttataatttg cattgtcgtc attatattgt gctcattatt
     3541 aagtccgttg ttaatcagaa cggggttgcg agagatcaaa aagttgagtg gcgtaacgga
     3601 agcgctgaat tataacgata gccgagtgcc ggttgaggtt aacgcattac cgagagaact
     3661 aaaacctctt gggcaggcgt tgaataaaat gcatcatgct ttagtcaaag attttgagcg
     3721 ccttagtcaa tttgctgacg atctcgctca tgaacttaga acgccaatta atgcattact
     3781 gggtcagaat caggttacgc tcagtcaaac cagaagtatc gctgaatatc aaaaaacaat
     3841 tgccggaaac atcgaagagc tggaaaatat ttcgcggtta acagagaata tactgtttct
     3901 tgccagggca gacaaaaaca atgttttggt gaaactggat tcgctttctc tcaata
//
LOCUS       DACADA010000264         3948 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.269, whole genome shotgun sequence.
ACCESSION   DACADA010000264 DACADA010000000
VERSION     DACADA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.269"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..652
                     /locus_tag="ISB17_004638"
     CDS             <1..652
                     /locus_tag="ISB17_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982484.1"
                     /translation="QDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINKDNIKVS
                     YSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIKLEWSGD
                     KRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWS
                     LYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(895..1590)
                     /locus_tag="ISB17_004639"
     CDS             complement(895..1590)
                     /locus_tag="ISB17_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAP1982485.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1583..3010)
                     /locus_tag="ISB17_004640"
     CDS             complement(1583..3010)
                     /locus_tag="ISB17_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAP1982486.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(3021..3740)
                     /locus_tag="ISB17_004641"
     CDS             complement(3021..3740)
                     /locus_tag="ISB17_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAP1982487.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1036 a         1029 c          829 g         1054 t
ORIGIN      
        1 acaggatatc gatctgattg agattttatt ttatctgaag aaaaagattc gtgttatcct
       61 ttttattata gctatctgta tggcaatggt gctgttgttt ctgtatatca ataaagataa
      121 tataaaagtg agttacagcc taaaaataaa tcagacaaca ccaggcatac ttgttagctg
      181 tgatagcaat aataactttg cctgtcagac cacaatgact gaagatgtta ttcagcgaat
      241 tactacattt tttcagacca gcccagatgt caagaacaga gaaataaagc tggaatggtc
      301 aggagataag agagatttac caactgctga agcggaaata tctcgcgtgc aggcgtctat
      361 cattaaatgg tatacgtcag aataccataa tggcaggcaa gttctcgatg agatacaaac
      421 gccttcagca attaacagtg agctttatac aaaaatgata tatctgacca ggaactggtc
      481 actgtatccg aacggtgatg gctgtgtcac tataagctca ccagaaataa aaaataaata
      541 ccctgctgcc atttgcctgg ctctgggatt ttttctaagt attgtgattt ctgtaatgtt
      601 ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac tctgggcagt aataaacagc
      661 attctgacag agagtgcctg tataaaacta tagtgacagt gaccgggaag ggagcccgtc
      721 ttcaggaatg gattcctgtc tttttaactg taaatatcct ggcattatta atgtcaggat
      781 atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg tccttaacgc
      841 tattcacaac ctcgtatacc gccttcaggt ttatgtccag actacatatc tctctcagca
      901 atcctcaata atcagataca ccgctttcac cggaccatga accccgacga ctttgataag
      961 ctcaatgtcc gccgttgaac tgggaccact gatgatgtta atacatgacg gcattcgttc
     1021 accggcctgc gctttctgat gcaatttttc tgcaagttgc gctacacgcg gcaggattgt
     1081 gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac gcccgcgccc
     1141 ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag catatacgac
     1201 gcccactata gcctgctctg cttgtgatat gttctcaata cctttcgccg gatcccaaac
     1261 aacggcattg cattcctgct gcaaacgttc gctaatcccc aattcctcca gcctcgtgtc
     1321 accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg cagcttctgc
     1381 cgccttcgcc tcgctggtca gttcacagcg cgtcaacata acatcgctgg caaactggat
     1441 aaacgcgtca cagcgctgtt gttgggtaag ttgggtaagc cgctcgttag catagttgtt
     1501 aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg cctgagcaac
     1561 gttattcaaa aattcgctcc gattatccat tcttattctc ctgcgcctga tgtttcttaa
     1621 accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc
     1681 taatcgcgcc aaatttgagt ggtgttttgc cgccattgat aaaccagctt gccgcatgag
     1741 caccagccat catcccaact ttccacaatc ctggatgact attggcataa gcgaacattt
     1801 ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc cgacgatgac
     1861 gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa gcggtgcata
     1921 aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtaac ggggaaatca
     1981 ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata tggcgatatg
     2041 ccggacaagt attcatacaa gccccgcaac gaatacagcg cagcacatcc cgaaattcag
     2101 aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct tcaggaccat
     2161 caacgtgccc cgcttcgcgt ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac
     2221 caacggcact gcgcgccagc atggtgatca atacatctac ctctgcaaac gtgggagcaa
     2281 tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac attcgcgcat
     2341 taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa ttacagccgg
     2401 taatacctat ttcagcactg aggaaatctt cgcggatttt ttgccgaatg aataaggtca
     2461 tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact cgacggatct
     2521 gatggcgatc tttatgaatt gccgggacca caacatgaga gggcggatct tgatccagct
     2581 ggagaatata ttcacccaga tcggtttcaa tcacctgaat cccggcatcc tgcaacacat
     2641 gattgacacc aatctcttcg gtcaccatcg atttagattt cactaccttc cgggcatttt
     2701 tgcgttgggc aacctgtaaa atgtagcggg tggcgtcttc tttggttttt gcaaaataga
     2761 cgtgaccgcc gttttgtgtc actttttctg agagctggta cagataagcg tcgagattac
     2821 tcagaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag tgccccaatt
     2881 catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc accgctttgc
     2941 gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta ttactggttt
     3001 tgatcgacat ctttatatcc tcagcggctc atcagcactt cagcaatatg catcactttg
     3061 actttctgcc cttcccgttg taatcgtcca ctgatgttca gcaggcaact cacgtcagca
     3121 ccaattaaat actcagggcg gacttccatc aggtgcgcaa ccttttcttt caccatctcg
     3181 ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca ggtatcctgt
     3241 tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag tggttcgtcc
     3301 ttcaccccca gcttacgggc caggctacaa gatgggtgat acaccgctct cccttgcaaa
     3361 ctggcaccta catcgactac ccctaatgta ttaacaataa aagaggtgag atcctgcata
     3421 cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag atacgtcggg
     3481 taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc gtcgttatcc
     3541 tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata accactattg
     3601 atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca gccgagtttt
     3661 tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc aatacaggtg
     3721 acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt atgcccgatg
     3781 gttccctcct tccgggggat caatcatcaa taaatatggc gttctttttt aattggctag
     3841 caacatacca cctctcattt gtatgacaaa tgataatctt tcatctaaac ttcaaatgca
     3901 ataactaatt ttatttcttt cggcatcgct cacattttat agggaaat
//
LOCUS       DACADA010000265         3909 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.270, whole genome shotgun sequence.
ACCESSION   DACADA010000265 DACADA010000000
VERSION     DACADA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.270"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            970..1623
                     /locus_tag="ISB17_004642"
     CDS             970..1623
                     /locus_tag="ISB17_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP1982488.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            1716..1973
                     /gene="pemI"
                     /locus_tag="ISB17_004643"
     CDS             1716..1973
                     /gene="pemI"
                     /locus_tag="ISB17_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HAP1982489.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            1906..2307
                     /gene="pemK"
                     /locus_tag="ISB17_004644"
     CDS             1906..2307
                     /gene="pemK"
                     /locus_tag="ISB17_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HAP1982490.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            2592..3683
                     /locus_tag="ISB17_004645"
                     /pseudo
     CDS             2592..3683
                     /locus_tag="ISB17_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(3699..>3909)
                     /locus_tag="ISB17_004646"
     CDS             complement(3699..>3909)
                     /locus_tag="ISB17_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP1982491.1"
                     /translation="MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     SLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1072 a          877 c          891 g         1069 t
ORIGIN      
        1 tacagccgcc tggccacagc ttccccctga aagtgacctc ctctgaataa ttcggcacgc
       61 gccggaggca ttcgcacgcc tgaagcccgc cagtgcacaa aaaatcagca ccacatacaa
      121 aaaaacaacc tcatcatcca ccttcaggtg catccggtcc cccttgtttt tgatacaaaa
      181 cacgcctcac agacgggtaa ttttgcttat ccacattaaa ctgcaaggga cttccccata
      241 aggttacaat cgttaatgtc ataaagcgcc agccgtcagc cctacagggt gcaatgtatc
      301 ttttaaacac ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa
      361 cctattcact gcttgtccta tggacagaca gatatgcacc tcccaccgta aacggcgggc
      421 cccaaccgga gccactttag ttacaacaca caaaaccaac ctccagaaaa accccgaacc
      481 agcgcagaac tgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc
      541 ccttcgggcc ggaacagagt cgcttttaat tatgaatgtt gtaactacat cttcatcgct
      601 gtcagtcttc tcgctggaag ttctcagtac acgctcgtaa gcggccctca cggcccgcta
      661 acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca
      721 gaagctctct catggctgaa agcgggtatg gtctggcagg gctggggatg ggtaaggtga
      781 aatctatcaa tcagtaccgg cttacgccgg gcttcggcgg ttttactcct gtatcatatg
      841 aaacaacaga gtgccgcctt ccatgccgct gatgcggcat atcctggtaa cgatatctga
      901 attgttatac atgtgtatat acgtggtaat gacaaaaata ggacaagtta aaaatttaca
      961 ggcgatgcaa tgattcaaac acgtaatcaa tatctgcagt ttatgctggt tatgctggct
     1021 gcatggggca ttagttgggg agccagattt gtcatggagc aggccgttct gctttatgga
     1081 tcaggaaaaa actatttgtt cttcagtcat ggtactgttc tgatgtacct gctgtgtgtt
     1141 ttcctggtat accgccgttg gatagctccg ctaccggtcg ttggtcagct gcgcaacgtt
     1201 ggcgtaccgt ggctggtcgg tgcgatggcc gtggtgtatg tcggtgtatt tctgctcggt
     1261 aaggcgctgg ctctgcctgc tgagccattt atgacgaaac tttttgccga taagtccata
     1321 cctgacgtga tcctgacgtt gctgaccatc tttatccttg ccccgttgaa tgaggaaacg
     1381 ctgttccggg ggattatgct gaacgtcttc cgttcacggt actgctggac gatgtggctg
     1441 ggggcgctga taacgtcgtt gttgttcgtc gccgcgcaca gccagtatca gaacctgctg
     1501 acactggcag aactgttcct ggtggggttg attacatcag tggccaggat cagaagtggt
     1561 ggcctgctgc tgccggtatt gctgcatatg gaagcaacca cgctgggttt actgtttggt
     1621 tgaaagttat atttttatta aacattgtgc gttaaagcct ggtgtgtttt tttagtggat
     1681 gttatattta aatataactt ttatggaggt gaagaatgca taccacccga ctgaagaggg
     1741 ttggcggctc agttatgctg accgtcccac cggcactgct gaatgcgctg tctctgggca
     1801 cagataatga agttggcatg gtcattgata atggccggct gattgttgag ccgtacagac
     1861 gcccgcaata ttcactggct gagctactgg cacagtgtga tccgaatgct gaaatatcag
     1921 ctgaagaacg agaatggctg gatgcaccgg cgactggtca ggaggaaatc tgacatggaa
     1981 agaggggaaa tctggcttgt ctcgcttgat cctaccgcag gtcatgagca gcagggaacg
     2041 cggccggtgc tgattgtcac accggcggcc tttaatcgcg tgacccgcct gcctgttgtt
     2101 gtgcccgtaa ccagcggagg caattttgcc cgcactgccg gctttgcggt gtcgttggat
     2161 ggtgttggca tacgtaccac aggtgttgta cgttgcgatc aaccccggac aattgatatg
     2221 aaagcacggg gcggaaaacg actcgaacgg gttccggaga ctatcatgaa cgaagttctt
     2281 ggccgcctgt ccactattct gacttgaaca tggggtttga ggggcaactg gatgaaaacg
     2341 tacgatttag ggcactaaaa ccgctgttgt cccaccattc tggtgattcc caaacgttat
     2401 ttggctaaaa agtagttttg atgtggttta ttttccgaat tccaagcgca gccctacttt
     2461 cttgtgcgta tttgcgtgtt ttgcgcagtt ttgaagttcc tacatcagat tgacttcgga
     2521 tacaaattca atgaacattg atgcaaatac tgacctatca gtttcttaac taaatactcg
     2581 agatacatct aatggctaat gaaaacttag atattgactc caagacagca gaagaattat
     2641 acgagtggta tctggatgat aaattaatta taaatcgaag ataccaaaga aaattagtat
     2701 ggtccctcga agagaaaaca gcactgattt catcaatgac tcagcaatac cctatccctt
     2761 tgctattgtt tgtttccatt gacaacaaaa gagaaatcct tgatggaatg caaagactag
     2821 aagctttaat gagttttatt gagcagcgat tttcattaaa tggtaaatac tttaatttag
     2881 atgccattgc attaacaaaa caactaaaag acagtggtaa acttcaacaa aaagagccag
     2941 tgctatcccg cgaaagttct acattaattg ctcgctatag atttgctata tctgaatact
     3001 cttcaacaga ggaccacatt gacgaagtgt ttcgacgcat aaattccaat ggtaaaattt
     3061 tatcaaaaca agaactacga agtgcggggt gcgttagtaa tttttcagag ttagtaagga
     3121 aaatatcgac aattataaga ggagatacta cacatagcga tataatggga ctgaataaaa
     3181 tacataacat atcaatttgc aatgatggac tcgattatgg aataaatata gacaatcatt
     3241 tctatattag aaatcatatc attagtaggc ctagcattag agactctgac gatgaagagt
     3301 tagttgcaaa tatcttgggc tatatatttt tagatgataa gccaacatca ggatcaacat
     3361 cgctcgatac attttacggt gaaggttcga cgagtcatgc aatccataca cgaactcaat
     3421 tagagaatta cattcagaca aatggtgcag ataaaattgt caataattac ctcttcgttt
     3481 atgagatgat tcaaaaatta tttgatgcaa ataatttgaa ctttagatca cacattctgg
     3541 gcaatgcatc ttcatctcaa gaatgtcctc gttactatca agctgttttt cttgctttgt
     3601 atgaattaat cataaatgaa aacatgcaac ttgacgatga acagaaattt atcgctcagt
     3661 taggagacag tgtacaacgt tcaggtaatg actccaactt actgatagtg ttttatgttc
     3721 agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc
     3781 cgtcccagcc ttgccagatg ttgtctcaga ttcaggttat gtcgctcaat gcgctgagtg
     3841 taacgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca
     3901 tccgtcatc
//
LOCUS       DACADA010000266         3885 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.271, whole genome shotgun sequence.
ACCESSION   DACADA010000266 DACADA010000000
VERSION     DACADA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.271"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            388..1878
                     /locus_tag="ISB17_004647"
     CDS             388..1878
                     /locus_tag="ISB17_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAP1982492.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1999..2571)
                     /locus_tag="ISB17_004648"
     CDS             complement(1999..2571)
                     /locus_tag="ISB17_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982493.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2657..2917)
                     /locus_tag="ISB17_004649"
     CDS             complement(2657..2917)
                     /locus_tag="ISB17_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAP1982494.1"
                     /translation="MFSSPTPVEINNIRRNIFMNTPVSLMDDQMVDMSFITQLTGLTD
                     KWFYKLIKDGAFPAPIKLGRSSRWLKSEVEAWLQARITQSRP"
     gene            3751..3885
                     /locus_tag="ISB17_004650"
                     /pseudo
     CDS             3751..3885
                     /locus_tag="ISB17_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
BASE COUNT         1026 a          844 c          999 g         1016 t
ORIGIN      
        1 cctgtccata gaatattgta ctcagcattc agtgcaggaa ctgtcatttt ctttatttta
       61 ttcttcttca ggagggcagt tttctgaaag cgttactccg gggaaactgt ttatgtttat
      121 tcccggcagg aaacccgtac ctgcaatacc ctggatgatg ctttttgctg gtacgttgtt
      181 gtgtgaaatt taatcaacgt gtgtgttccg gagagcgccc gtagacgcac agattcattc
      241 tgacattctt tagcgacaaa ataccgaatg gttattattt aatctgaaaa tcgacagaaa
      301 tttttatcct gtattgattc tgctgctatt tttgctatta gtagcaatag ttgcggtact
      361 ttatgttctg tgtgttgtga ggtcattatg ccatactttc agtatcctga tgagtttccc
      421 ctgagctcgc tgcctccact cattcgtgat gccgttattg aagctcagca aatcacgcag
      481 gctcccctgg ggcttgtggc agcatctgcc ctgggcgctg tttcacttgt ctgccagaat
      541 ctgattgatg tctgcagact gaatacacta cgggggccgg tttctctttt cttactgacg
      601 cttgctgagt ccggagaacg aaaaaccgca gtggacaaat tattaatgga gcccctgtac
      661 cagcaggaga tgctgttata ttccagacac aaaaatgaac tgacgacatg gaaaaataag
      721 gaagagcttt taaaggcgca gaaaaaagca ttgttgtcaa aactgaataa agaactccgc
      781 aaaggcgcgg atgagtcaga aacgctcaga caactggaag cgcttcagaa aaacagaggg
      841 gaaaaacctg taagatataa attcattttt aatgatgcca cgactgcggc aattaagaat
      901 cagctttgtg gtaaatggcg ttcagtgggt attatgtcag atgaagccgg tataatattt
      961 gatgggtata ccctgtctga gctgcctttt attaacaaaa tgtgggatgg ctccgtattg
     1021 tcggtggaca gaaagaatga acccgaacag atgattgaaa atgccagaat gacattatcc
     1081 ctcatggtac aacccggtct ttttgaccga tatatggagc gaaaaggttc tgttgccagg
     1141 gacagtggtt ttcttgcccg ttgtctaatc agtaaacctg ccaccacgca ggggaaacgc
     1201 tttattaacg gagctgtgac cccgggagga tcattaacgg catttcatga acgattaatg
     1261 gagctggcca gaggcagtat tgaaaaatcc agtaaggatg aacgttattg tctgcatttt
     1321 tctcctgaag cgcaaaaaat atttattgat cactataatg ttctggagca ggatttgtct
     1381 ccttccggac ccttaagcca gtttcgggga catgtttcaa aaaaaacaga aaacattgcg
     1441 agaattgctg cgttattgca gtatttcagt cagggtgaag ggaaaatctc tgccgatatc
     1501 atgacgtctg ctgtcgtaat aagttcgtgg tacacagatg aatataaaaa attatttgcg
     1561 ttacctgatg aatctgaact gcagctgcag gacgcccggg agctgctcga ctggctcatt
     1621 gaggagtgcc gcggagaatg cccgccccgt gtcaggaaaa attatattct ccagtgtggc
     1681 ccgggccggt tccggaacag gaaaaagctg aacgtcctgc tgaacattct tgcatctcaa
     1741 ctcaggctct ctgtcgtgca ggaaggaaaa accacctatg tcctgctttc cgaaataaac
     1801 aatatcacgc tgcctgaact gaatgcgtgc tacagccaga aactgataag atggcatgag
     1861 caatcctttg gcttataatg ttccctgttg ctgagacgga ttattcagtg gtggtatcgc
     1921 agtatgcccg tcacccccgc ttctgcatga gggtgacgga ggaaaagtaa gtgtgcggag
     1981 gcccgggagc gttatgaatc aacaggcgta taacaggtac tcgtcaatca tcagttccag
     2041 ctcgcgcttt tcccgtttgc gcgccacagc ccagttttca agctctgcat gtgatttatg
     2101 ctttaactct tcagtccagg gctgcatgga gtgggcacac atcagatcat accagcacag
     2161 ccatatcagc gtgtggtaga gatcacgatc gtcgaaacgc cggagcaggc tgttcagcct
     2221 gtttgccagc cggttttcac ccagacgatg tagctcactg agtaaaacgc cgcccagatt
     2281 aaaatgtgta agcagcatga tttcatgggt ctgaatgctg gtcattaacc cttcctgaat
     2341 ggcaggaatg aacggtggtg ttcccgggtt gacgacctga tagagtccct gtggtgccac
     2401 tgagatgatg gtgccttttt ctggcagaaa aaagtgggcc agggtcaggc ggtgataacc
     2461 atgaagatgc ggagccccca ttccatgagc gacgataagc ctcgtcacgt ttgtcatcca
     2521 tttctgatgc tggatgagca gcagctcaca ccggttttca agtttttgca taatattctc
     2581 tcctttagcg cgcagcaaaa tgcctccggg ggccggatca tgcctttgct gcggttgaac
     2641 ggataacggt caggaattac ggacgggact gtgtaatacg tgcctgcaac caggcttcca
     2701 cttcactttt cagccatcgg gagctacggc ccagtttgat gggggcagga aaggcaccat
     2761 ccttgatgag cttgtaaaac catttatcgg tcaggccggt cagctgagtg ataaacgaca
     2821 tgtcgaccat ctggtcatcc atcagtgaaa ctggggtatt catgaaaatg ttcctccgga
     2881 tgttgttaat ctcaaccggc gttggtgatg aaaacaacgc accaccttcc ccggcataac
     2941 aaccggggaa aaacgtaaaa aataatcggg aatgtgaccg tcaggagata aacgggggga
     3001 ttgcggtggc tgagccgtac cggtatgtcg ggtgattgtt gcgaaacgat gcttccgcac
     3061 gcgcacgtga taaaggaggg aggactttcg tgagacaagg ggaaaactgg cgttctgata
     3121 attgcagagt tgtttttgaa ggcggttgtg gtgcatcagc ccgtgacctg tgggcaggcg
     3181 acaggtatca ctggtgggat aagactgtct gattggtatt ataaatatat aagagagctc
     3241 aaacacatga gttcatatat gtgaaataaa tctgtgagtg tgatcagcag accagtctac
     3301 ccagtagcaa cagattgcgt tgtactcaca gggttatccc tgataactgg cgtataacct
     3361 gcgtacataa aacgtacctg caatgcacct gaacagagtg cattaagcat ccgcctgagc
     3421 ggtaaacgta tccgatctgg tgagtgacag tctgagagca ttctcagtaa tcgccccctt
     3481 gtatcctgga aattggttaa gataacagcg attagatttg gcaaggtgta tgcagccagc
     3541 agcttaacat ctcagctaac tggctggtta ccttcaggtt gtcgcaatgg catcggacat
     3601 ccggtgtcgg ataactaaac gtagcgttaa ggcgatattg ctgagagacc ggttaagtgg
     3661 ttgcacggga gtaatgagtt tggggagggt accagaatgt cagtctggtg cggtatgctg
     3721 gcagtatcac gttgattaca gcataaagta acgagagacg aatggaagtt aaaaccagag
     3781 cagttccgtc gttgttcatc ccgggagacg ctggaaaaag tgatttccca cacacgttat
     3841 aaacttaccc ctgcggagct ggaagccttt aactctgcgg tcgat
//
LOCUS       DACADA010000267         3880 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.272, whole genome shotgun sequence.
ACCESSION   DACADA010000267 DACADA010000000
VERSION     DACADA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.272"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            22..1680
                     /gene="dpiB"
                     /locus_tag="ISB17_004651"
     CDS             22..1680
                     /gene="dpiB"
                     /locus_tag="ISB17_004651"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HAP1982495.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1649..2329
                     /gene="dpiA"
                     /locus_tag="ISB17_004652"
                     /gene_synonym="citB"
     CDS             1649..2329
                     /gene="dpiA"
                     /locus_tag="ISB17_004652"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAP1982496.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2370..3755)
                     /gene="dcuC"
                     /locus_tag="ISB17_004653"
     CDS             complement(2370..3755)
                     /gene="dcuC"
                     /locus_tag="ISB17_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP1982497.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1055 a          930 c         1005 g          890 t
ORIGIN      
        1 ggacaacccg caaggaaaac aatgttgcag cttaacgaga ataaacagtt tgcatttttc
       61 caaagactgg catttccgct gcgtatcttt ttgctgattc tggtgttctc aatatttgtc
      121 attgcagccc tggcgcaata ttttacggcc agttttgagg actatttaac gcttcatgtg
      181 cgcgacatgg caatgaatca ggcaaaaatt attgcttcta atgacagtat catcagcgca
      241 gtgaaaacgc gtgactacaa gaggctggcg accatcgctg acaaattaca aagagatact
      301 gatttcgatt atgtggtgat tggcgaccgg cactcgattc gtctttacca tcctaatccg
      361 gagaaaattg gttatcctat gcagttcacc aaaccgggcg cgctggagaa aggggagagc
      421 tacttcatca ccgggaaagg gtcaattggc atggcgatgc gtgccaaaac gccaatcttt
      481 gatgacgatg gaaaagtcat cggtgtggtg tcgattggct atctggtgag taaaatcgat
      541 agctggcggg ctgagttttt attaccgatg gcaggcgtgt ttgtcgtgct gttagggatt
      601 ttgatgttgc tatcgtggtt cctggccgcg catatccgtc ggcagatgat gggcatggag
      661 ccaaagcaaa tcgcacgcgt ggtccgtcag caagaggcgc tgtttagttc ggtttatgaa
      721 gggctgattg cggtggatcc gcacgggtac attaccgcca tcaatcgtaa cgcaagaaag
      781 atgctggggc tgagctcccc cggacggcaa tggttgggta aacccattgc tgaagtggtc
      841 aggcccgccg atttctttac cgaacagatt gatgaaaaac gtcaggatgt ggtggcgaac
      901 tttaacggtc tgagcgttat cgccaatcgg gaagctattc gttctggcga tgatttgctg
      961 ggggccatta tcagctttcg tagtaaagac gaaatttcca ccctcaatgc gcaactgacg
     1021 caaattaaac aatacgtcga gagccttcgt acattgcgac acgagcatct caattggatg
     1081 tcgacgctca atggtctgtt gcagatgaaa gagtatgatc gcgtgctggc gatggtgcag
     1141 ggtgagtctc aggcccagca acagcttatt gacagcctgc gtgaggcgtt tgccgatcgc
     1201 caggtggcgg ggctgctttt tggtaaagtg cagcgcgccc gcgaactggg gctaaaaatg
     1261 gtcatcgtcc ccggtagcca gctttcgcaa ctgccgccag gactggacag caccgagttt
     1321 gcagccattg ttggcaattt acttgataac gccttcgaag caagcctgcg tagcgatgaa
     1381 ggaaacaaga ttgttgaatt atacctcagc gatgaaggcg atgatgtggt gattgaagtc
     1441 gccgatcagg gctgcggcgt tccagagtct ctacgagaca aaatatttga gcagggtgtc
     1501 agtacgcgtg ctgacgagcc cggtgaacat ggcattgggt tgtacttgat tgccagctac
     1561 gtaacgcgct gcggtggtgt tatcactctc gaagataatg atccctgcgg taccttattt
     1621 tcaatctata ttccgaaagt gaaacctaat gacagctcca ttaaccctat tgatcgttga
     1681 ggacgaaacg ccgctggcag agatgcatgc ggaatatatt cgtcacattc ccggattcag
     1741 ccagatatta ctggcgggaa acctggcgca ggcccgaatg atgatcgagc gttttaagcc
     1801 ggggctaatc ttgctcgata actatcttcc tgacggtaga gggattaatt tactgcatga
     1861 actggtgcag gcgcattatc ccggcgacgt ggtgtttacc actgcagcca gcgatatgga
     1921 aacggtgtct gaagccgtac gttgtggtgt atttgattat ctcattaagc caattgccta
     1981 tgaacgtctg gggcaaacgc taacccgttt ccgccagcgt aaacatatgc tggaaagtat
     2041 tgatagcgcc agccagaagc aaattgatga gatgtttaat gcctatgctc gcggtgaacc
     2101 caaagacgaa ctgccgaccg gcattgaccc cttgacgcta aacgcggtgc gaaaactgtt
     2161 caaagagcct ggtgtgcaac atacggcaga aacggtggcg caggcactga ccatcagtcg
     2221 caccactgcc aggcgttatc ttgaatattg cgccagccgc catctgatta ttgctgaaat
     2281 tgttcacggc aaagttggca gaccgcaacg catataccac agtgggtgac atgataaagc
     2341 cggaggaaac ttgcctccgg cattactatt tacttgcctg tgaccgctgc tgccgttcct
     2401 ggcaccatca gctctgtagc aacgataaca atcaccaaac caacaagcac cggcaccgag
     2461 gtgcgtttta cgacttcaaa cggcgagatc ttcgccatcc cggcaaccgc aacgactacg
     2521 ccagaaacgg gtgatagggt acgacccagg tttgacgcct gcagcatcgg gatagtcaaa
     2581 tacgccgggt taatgccgga ggagtgcgcc agtttcggga tcatctcaac aaacgcataa
     2641 aacggcgcat tgcctgaacc ggtcgtgact gccgccagca ttgtcaggat caccaatacc
     2701 agcatcagga tgatactcgc cgaaccaaac gaggtagcga tagaaatcag actttgaata
     2761 aagccgatgg tgctaagccc ctgagcgaat accccagcgg caaccagcag catcaccacg
     2821 ttagcaaatg catctgccat accgcgataa gccacttcca gaccagagaa aactttctgg
     2881 gtattaaagc tgcggatgaa ctccagaatg gaggcaatta gcatacaaat caccagaata
     2941 gtgatgatgt gtaattgcgg accccatttg ccgtcaaaaa tcagcactcc gatgatcggc
     3001 gtgaacggca aaatggcata aaacgcaggg gcagtggtgg tgatttcact gacatctaac
     3061 atttcatgag agatgtgctc ttttttatcc agataacgtt gccagaagaa gtgggcgatc
     3121 gccatgccga taattgcagc aattgagata ggcagcgttg ttttgaaggc gaagtcaatc
     3181 agcgacattt cggaagcctg cgctgccagc accacatccc ctgaagtcgg tgcgagaata
     3241 atcgccgccg gggaggcaca aatggcagca gctgcgccac gactgatacc aacgtttacc
     3301 atcaccggaa acagggttgc catcagcaaa acacccagac cggttgcgga agagacggcc
     3361 agtgacatca gacaggcaac aaaataggcg gcaatcatca gaaggtaagg ggagttaata
     3421 tactgcaatg gttttgacgc cagcttgacc accatatcat tcgcgccgat atgggtcatg
     3481 taagcggcaa agccacacag catcataatc atcatgccga ggtcgccgcc gcggctcatt
     3541 agcaatattt taacgtattc aacgatatcc gtggcgctgt agcctgttga agcctggctg
     3601 gacggtaaca ctttgtgccc cataatggca ctgataatca gcaataacag gccaccgaca
     3661 aataacacgc cagtggcaga atacccttta atgatgtagc gagctacacc cacaataacc
     3721 acaaccccaa taaggagttc aatgaatgtc agcataattt ttcctgtctc caggccccaa
     3781 agtaaataat aaaaaattcc taaaagttaa ggaaaaaata tgcccaataa attggcggtg
     3841 aatgctgatt aaaatcaaga aaaactgcca ttaagacatt
//
LOCUS       DACADA010000268         3877 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.273, whole genome shotgun sequence.
ACCESSION   DACADA010000268 DACADA010000000
VERSION     DACADA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.273"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..391
                     /locus_tag="ISB17_004654"
     CDS             <1..391
                     /locus_tag="ISB17_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HAP1982498.1"
                     /translation="QDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGV
                     RRIDDQLLHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPC
                     TTRVLDSGAHTSDELAAMVLDGIDGRT"
     gene            complement(873..1064)
                     /locus_tag="ISB17_004655"
     CDS             complement(873..1064)
                     /locus_tag="ISB17_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982499.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     gene            1088..1315
                     /locus_tag="ISB17_004656"
     CDS             1088..1315
                     /locus_tag="ISB17_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982500.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     gene            1366..2502
                     /locus_tag="ISB17_004657"
     CDS             1366..2502
                     /locus_tag="ISB17_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HAP1982501.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRD
                     PKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRIIRIGEDWS
                     EEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQNISAVLDA
                     LGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVE
                     PPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPEL
                     FQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAG
                     DQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
     gene            complement(2469..2618)
                     /locus_tag="ISB17_004658"
     CDS             complement(2469..2618)
                     /locus_tag="ISB17_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982502.1"
                     /translation="MLFAPTLADKAILPTAWGGRYFFNRIGRTQPFGSTEPCLYPTKL
                     RKLAR"
     gene            2617..3877
                     /locus_tag="ISB17_004659"
                     /pseudo
     CDS             2617..3877
                     /locus_tag="ISB17_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISCfr1 family transposase"
BASE COUNT          798 a         1135 c         1125 g          819 t
ORIGIN      
        1 tcaggatctc cccttgtgga ggggacttac gatcgcggcg gtcagggaga ttcgaaggaa
       61 ttactcgcag gacatcatca tcccaatgac gctcgtgcac ccggactatc tgactgagat
      121 actcgacggg gtaaggcgga tcgacgatca gctgctgcac atctttctga cgctcaacga
      181 ggacctattg cgtcaccgga tcgcgaacca gaccatgcat cctgacccga atcgaaatgc
      241 ggagattcga gagtggcgat tagcgaatgt cgcccgatgc ttggccgcaa gggaacggct
      301 tccatgcaca acccgtgttc tcgatagtgg tgcacacacc agcgatgaac tcgcagcgat
      361 ggtgctcgac ggaatcgatg ggcgcacctg atcgccttcg acgcctgcgc aaagcgtagc
      421 gcgagggtgg cgggctcacg accaaacgcc cagaggtcga tcatcgcagg gatgtttggc
      481 tttgtggtgc ggacgacggg actcgaaccc gtactctcac agagaagcag attttcgtac
      541 cacctcgact ttcgccgccg tctgatgacg ttcgtggtct ggactgtccc ttcgccattg
      601 cccgaaggct ttaggcgccg cccgtccagt ctctacacct tcccccgaag gggcttggct
      661 cgggattggc ttagggtatt gcccgttagc gttccccgac tttgagcggt tctactccgc
      721 ggatttcccc gcgggcactc caattttaaa gtctgctgcg tctaccgatt tcgccacgtc
      781 cgcctttttt cgccgttcct agcgctcgtg cgatgcacct atgttgcacc tagcgccgaa
      841 tcgttcttcg tcatcctgaa aaaccacgtc tcctaaagcc ttgcatagct tatcttttct
      901 ccaccacgaa cttttttgtg ggatggtaga aaaaaagact ttttaagtcc gctggcttgc
      961 caggccttgt tagcttgtac ggtcatggtt atcgggtaaa gaatattgac ggcatcgctg
     1021 gtgtcggtgg ctgaaaagcc ggctcccatc agggcaatag ccatttcaga tgcaggcgta
     1081 cagggcaatg gtcaacagct acagcctgtc tgacgattcc ggcgtcatgg ctgcggcggc
     1141 tatcacgcat tttttgttcg gtcaggcggt gttttcgtac ctcaatggtt ggagcgtgtt
     1201 gatcggacct ggtacaggtt tggacagcac gggctgcaaa tacgcaaggg atttaatggg
     1261 cctggtggcg ttcacggctt ttatcgtgac gtttctgttc aggggctact cataatctcg
     1321 tggctcggcg gttcccggca caccatgaca gtaaggaagg accctgtgtc tcaactctcc
     1381 cagcttcgaa gccccgccgc cgtgcaggct gccatcgatg agttcgtgca actgggccgc
     1441 acgaaattcc tggcgcgcca cggctacggc aagtcccgcg acttcctggt acgtgatccg
     1501 aagaccggca ccgattgcga ttccaaggcc atcgccggtg tggccttcgg caagcaattt
     1561 cccgagcagg gcccgctcac tgctgacagc ttctccggtg gcgaggcgac cgtcgttccg
     1621 gcgctgacgc ggctcgggtt tcgcatcatt cgcatcggcg aagactggtc cgaagaagag
     1681 gtcctggcca cggtcgaaga ctatttcgac atgctgcgtg ccgaggcggc tggggagccg
     1741 tacaacaagt ccgagcacaa ccaggcactg cgccaactgc tgaacggtcg cagcaagtct
     1801 tcagtcgagc tcaagcacca gaacattagc gccgtactcg atgccctggg cctgccctat
     1861 atcaacggct acaagccacg cggcaacagc caactgctgc tgcgtaaatc cgtacacgcc
     1921 tacgttctgg aacatcagca gacggtcggc gctcttgtcg atgccctgga ggaggtaaaa
     1981 cttccgggtg acaaaaccta ccgagcggct ttggtagaac cacccgcccg tgaagtgctt
     2041 gtgcgtaccc cggcatctct acggcaacgc ctaccgcgaa agttcgatta tgccgctcgc
     2101 gatgaagcca accgcaagct gggccgggca ggggagcagt gggtgattgg ctacgaacag
     2161 caacgcctga ccgagctcgg ccacccagag ctttttcagc ggctggattg ggtgtccgac
     2221 acccagggag acggtgcggg gttcgacatc ctgtcgttcg aagaggacgc ccatgagcgc
     2281 ttcatcgagg tgaaaaccac caatggcggg gtaggctcgt ctttcttggt cagccacaac
     2341 gaactcgaat tctccaagga ggcgggcgat caattccatc tgtatcgcgt gttccagttt
     2401 cgggacggtc cgcgcctgtt cacgctaccc ggcgacctca gccaacatgt gcatctcaag
     2461 ccgacggact accgggcgag tttccggagt ttggtggggt aaaggcaggg ttctgttgag
     2521 ccgaatggct gtgtgcggcc gattctgttg aaaaagtagc ggcctcccca tgccgttggc
     2581 aaaattgctt tgtcagcgag cgtgggggcg aacagcatga tgggacagtt accgggagga
     2641 cagcagcgcc tgttctactc gttcaatctg gaagatcacg tcccggccca acatctcctg
     2701 cgcagcatcg accagtgctt ggatctcagt gatctacgtg cctacctggc agatttctat
     2761 agccccatcg ggcgtccctc gattgacccg gagttgatgg tgcgcatgct ggtcgtcggc
     2821 tacttgctat ggcattcgtt ccgagcggcg attgtgcgaa gaggtgcacc tgaacctggc
     2881 ctatcgctgg ttctgccggt tgggtctgga agacgaagtc cccaatcact cgaccttctc
     2941 gaagaatcgc catgggcgtt ttcgtgacag cgatctattc cgctggttat tcaatgaggt
     3001 gctgcggcgc tgcatggcag ccggcctagt caagggtgaa ggtttcgccg tcgacgccag
     3061 catcattaag gcggatgcca gccggcaacg tggggtggcg ggagatgagg tcgattggaa
     3121 cgatccaaag ctcagcagcc gcgcagtgcg cgagtacctc gaagcccttg atgaagaggc
     3181 gctggctgag gctcttccca agaaaatttc gctcactgat cctcagtccc gttggacagc
     3241 agcgccaggt ggcccggcct tttttgccta ctccacgaat tacctgatcg acactgagca
     3301 cggtgtgatc atggacgtgg aagctacccc ggcgcaccgt accgccgaag tcgattcgac
     3361 taggacgatg gtcgagcgtg tcgaggcgca gttcgatctc acaccggaac gccttatcgg
     3421 cgataccgct tatggcaccg ccccgatgct ggcctggatg gtcgaagaaa aggacatcga
     3481 accgcatgtg ccggtgtggg acaagaccga gcgcaaggac gacagcctct ccagtaacga
     3541 ctttcactgg agtcaggacg ccaatgaata tcgctgccca gccggcaaac cgctacgcag
     3601 tgaatggcgc gccttcaccc agcaaaggtc gcgggtaact aaggccaaaa ccgtcattta
     3661 ccgctccagc caaaccgact gcgccacctg cccgttgaaa gcgaaatgct gccccaacac
     3721 gccgaatcgg aagatcgtcc gcagcatcca tgaggctgcc cgcgacgtgg ctcgacgcat
     3781 cgccaagaca ccggagtacc tcgtctctcg ctgcgaacga aagaaggtgg agatgctttt
     3841 cgcccacctc aaacggatca tgaaactcga ccgttta
//
LOCUS       DACADA010000269         3822 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.274, whole genome shotgun sequence.
ACCESSION   DACADA010000269 DACADA010000000
VERSION     DACADA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.274"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            209..1447
                     /gene="alaC"
                     /locus_tag="ISB17_004660"
     CDS             209..1447
                     /gene="alaC"
                     /locus_tag="ISB17_004660"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAP1982503.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1512..1583)
                     /gene="ypdK"
                     /locus_tag="ISB17_004661"
     CDS             complement(1512..1583)
                     /gene="ypdK"
                     /locus_tag="ISB17_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAP1982504.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1940..2860)
                     /gene="lpxP"
                     /locus_tag="ISB17_004662"
     CDS             complement(1940..2860)
                     /gene="lpxP"
                     /locus_tag="ISB17_004662"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAP1982505.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3213..3455
                     /locus_tag="ISB17_004663"
     CDS             3213..3455
                     /locus_tag="ISB17_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAP1982506.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3532..3807)
                     /locus_tag="ISB17_004664"
     CDS             complement(3532..3807)
                     /locus_tag="ISB17_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1982507.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
BASE COUNT          975 a          890 c          963 g          994 t
ORIGIN      
        1 attataatgt taataaaatg agtggtaatt ttcttgttag cgtagataaa tttcctgttt
       61 ttgaagtgga gttcctcatt taaagccatt gataaaaatg ttctaaaacg ctggacagac
      121 agaaattaat caggctattt tcaaaacagc caccatcgtg gcagcgtcgc tcggacggtc
      181 cgggcgctaa cgttaatctg aggaaattat ggctgacact cgccctgaac gtcgctttac
      241 gcgcattgat cgtctcccgc cctacgtttt taacattacc gctgaactga aaatggctgc
      301 gcgtcggcgc ggcgaagata ttatcgattt cagtatgggt aacccggacg gtgcgactcc
      361 gccgcatatc gtcgaaaaat tatgtacggt ggcccagcgc ccggacacgc atggttactc
      421 cacttcacgc ggcattccgc ggttacgtcg cgccatttcc cgctggtatc aggatcgcta
      481 cgacgttgaa atcgacccgg aatcagaagc catcgtcact attggttcga aagaggggct
      541 ggcgcacctg atgctggcga cgctggatca tggtgacaca gtgctggtgc cgaatccaag
      601 ctaccccatt catatttacg gcgcggtgat tgccggggcg caggtacgct cagtgccgct
      661 ggtggaaggt gtcgacttct tcaatgaact ggagcgcgcc attcgtgaaa gttatccgaa
      721 accgaagatg atgatcctcg gcttcccgtc aaacccaacc gcgcaatgcg tggaactgga
      781 attctttgag aaggttgtgg cgctggctaa acgctacgat gtgctggtgg tccacgacct
      841 ggcctatgcc gatatcgtct acgatggctg gaaagcgccg tcaatcatgc aggtgccggg
      901 cgcacgcgac gtggcggtcg agttctttac gctgtcgaaa agctacaaca tggcgggctg
      961 gcgtatcggc tttatggtcg gtaacaaaac gctggtcagc gcgttggcac gtattaaaag
     1021 ctatcacgat tacggcacct ttacgccgtt gcaggtggcg gctattgcgg cgctggaggg
     1081 cgatcaacag tgcgtgcgcg atattgccga acagtacaaa cgccgccgcg atgtgctggt
     1141 gaaagggctg catgaagcgg gctggatggt cgaaatgcct aaggcttcga tgtatgtctg
     1201 ggcgaaaatc ccggaaccgt atgctgccat gggctcgctg gaatttgcca agaaattgct
     1261 taacgaagcg aaggtctgtg tctctccagg gattggcttt ggcgactacg gcgacacgca
     1321 tgtgcgcttt gcactgattg aaaaccgcga ccgtattcgt caggcgattc gtgggattaa
     1381 agcgatgttc cgtgccgacg gtttactacc cgccagcagc aaacatattc acgaaaacgc
     1441 ggaataaacg ccttcgccag aaatgcaaac aggagcctaa tggctcctgt ttttcattgc
     1501 acagtttgct tttagatcat cagggcaaaa gtaccggccc aaacgatgac cataaaagaa
     1561 atgcccataa agaaatattt cactttacat ctctccgcgt aattactgtt acgcctgaat
     1621 gacttcatta cacctgatac agtgagttga caccgggcaa aacgacctta aacgtcctgt
     1681 attttaccgt gggcatcatc ccagaattct gagctaaact gctcgagacg gcgctaatgt
     1741 acgcctaaaa ctgaaacgag acaagaggca ttttcttatt tacttttagt aacttacagg
     1801 tgtctcatat cggcgacagt ttaatttctg acggtaataa ttagatattc agcgaaggaa
     1861 aaaaccttaa gaaaaaaagc cataaaaacc atgaggttat tatggctgat ttgaggaggg
     1921 aaagagtgag agccgtttgt taaatgtata acgacgattc acccaccggg cgcgttttaa
     1981 agcgacggtg tatccagagg tactgctccg gtgcgcgcat gatctctttc tcgataatct
     2041 tgttcatata ggcagcggct tgattttcat ctgtcgggta gccttccatc tctggggtga
     2101 tgtacaaacg atatccgctg taatccgctt ttcttaccat cgttacggtc aacatggctg
     2161 cgccagagag acgagagagg acataggtgc cattggttgt ggcgacattt tccaccgcaa
     2221 agaacggcgc gaaggagctg cctttacgac cataatcctg atcgggagca aaccataccg
     2281 cttcaccttt cttcagtgca ctgacaatgc cgcgcagatt atttctgccg atcatcgctt
     2341 tgttagagcg catgcgacca cgggtctgga cccattccat cagctgatta ttatgcgggc
     2401 gataggtggc catcattggt tggcaaagtc ccatcacccg accgcccagt tccagcgaca
     2461 taaaatggac gccgacaacc attacgccgc gattttgcat ttgtgcgcgt ttaaggttat
     2521 ccagcccttc cacatcaaac catttacgta cgcgactgtc gggccagaac cacgccatgc
     2581 cggtttctac cagcgccatg ccgagtgaac gaaagttttc ggcaatcatc ttctcgcgct
     2641 cttccgcaga atgctgcggg aaacaaagtt ccaggttttt acgggcgata gattcacgac
     2701 gtttcaggaa tggtcgcgcc attgtgccga tacgcgtgcc gagaaagcag agaacaggat
     2761 aaggaagctg tacccaaagc cagagtacac caagcccaaa ccacgtgagc cagtagcgag
     2821 gatgcagaaa ctcgcgggaa aatttgcatt ttggaaacat agttaccttc tgtagatacg
     2881 aattgttgta tcgcagaaga ttccatgtcg acagcggtac actcaattga ttaggccatc
     2941 atgtacgaca atggttcctg taaagagtgt aaagactagt caggacataa ccacgaggag
     3001 catgaatgcg gtatctgtac tttatcactc gctgactgct ttcagctact gattattaat
     3061 cgtgcaaagt aaatattttt ccgtacgtaa cagtcttgca tttaacatgc tgttttgatt
     3121 gatgcttagc aaaaattgcc agaggttaag gattgtctta ctattaaagt gactgaagtt
     3181 acaggttttt tagcgcggca ggaggttgag ctatgattta tttatggatg tttctcgccc
     3241 tgtgtattgt ctgcgtaagc ggatacatcg gtcaggtact gaacatggta tcggcgatct
     3301 cttctttctt cggcatggtg atcctcgctg cactcattta ttacttcacc atgtggttaa
     3361 ctggcggtaa tgaactggtg acggggatat ttatgtttct tgccccggct tgtggcttga
     3421 tgattcgctt tatggtgggg tatggcaggc gatagtgggt caggatggct catttgggca
     3481 ggtgttcaca tcagcatcag acatgattgt tcacctgcca ggtgacagac gctatcgcaa
     3541 aggatgaatg agataattcg cctttgtcgg ataagtaggc gtgatgtctt ctgtgaggtt
     3601 taatggtggt gcatcttctc tcatcttaaa caaaatcgcc tgtgatggct gaagttttgt
     3661 agaatgcgtt gtcgtcatca ctgacgtttt ttctgtctct ttatagctcg cctcactacc
     3721 accaccagaa atagaaaatg caaaaacatt gcatgccata atagagacaa ataaaaataa
     3781 gctaaaaagc attaagttta ctttcataga aaatatcctg ga
//
LOCUS       DACADA010000270         3680 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.275, whole genome shotgun sequence.
ACCESSION   DACADA010000270 DACADA010000000
VERSION     DACADA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.275"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..317
                     /locus_tag="ISB17_004665"
     CDS             <1..317
                     /locus_tag="ISB17_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1982508.1"
                     /translation="SPFNIKRCLGKVPVTFVPRTIAIIDFVALINGSYCSVPEADVSL
                     SRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHINY
                     LV"
     gene            complement(374..1402)
                     /gene="chuS"
                     /locus_tag="ISB17_004666"
     CDS             complement(374..1402)
                     /gene="chuS"
                     /locus_tag="ISB17_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HAP1982509.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(1451..3433)
                     /locus_tag="ISB17_004667"
     CDS             complement(1451..3433)
                     /locus_tag="ISB17_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HAP1982510.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT          940 a          943 c          850 g          947 t
ORIGIN      
        1 tctccccctt taatattaaa cgctgcctgg ggaaagtacc ggtgaccttt gttccgcgaa
       61 ctatcgccat catcgacttt gtcgcactca tcaatggcag ttactgctct gtgcctgaag
      121 cggatgtgtc cctctcgcgc aagcagcatc aggttctgag ctgtattgcg aatcaaatga
      181 caacggaaga tattctggag aaactgaaaa tatcgctaaa aacgttctac tgccataaac
      241 acaatatcat gatgatcctc aatcttaagc ggatcaatga gctggtacgc catcagcata
      301 ttaattatct ggtgtgaacg attgatcaat ataaagaggc ccagcaacag ccagacctcc
      361 cgttaattat acgttatgca gtaacgcctt ccggtatcaa cgaagcaatt tgcttacgcc
      421 attgcgcttg ctcctgttca ccttctgtac gttgaccata aagttgcgct atctgcgtac
      481 catcatgggc aaacaattcc agactggtta cgtgaccatc gctggtcggt ttacgggtaa
      541 cccaggcttc agcaatgctc tcttctaata gatgaagagt aaacgtcggg ttgaaaatat
      601 tcagccaacc tttcattggc accacttttt ctaccacacc ggtgaaaatc tgtacgcagc
      661 cacggttgcc aacaaacacc atgatttcat tgccatcctg ctgtgcagat tcaagaattt
      721 gcgccaacgc actgttggat actttgcagg ccaaatcgtc tgccaccaga ttgaacgcct
      781 gttggcgcgt cagattgtga cgcttgagca acgtaaaaaa ctgatgaacg tcggtcatcg
      841 cccgccactc ttgctcgacc acactggcat cggctcgcgt ttgaacaact ggcgcatcaa
      901 cggcttttaa ctcaagcggc gtattctcat cggtgataaa ccgtgccaga agctcactcc
      961 atgccgccat atcggtattg tcggtggcat aaacttttag taatgcatcg ccctgatgat
     1021 caaagaactg aatactctgg cgttcgccac gggccgtgtt ttctttgatg tggaaaacac
     1081 tggcccattg attgagaaac agacgtaaat ccagcgcgcg cggattgagg atcaatccgg
     1141 catgtccgtt caaatgctgg tttgtgaacg caccaacttg ctcatggact gcatattcat
     1201 tacggcaaat acatttggtt tcgccaacac tttcgagcgc cgccagaatt tcacgaatat
     1261 cgccgcgcat ccgccacgcg tcgtgcgtga cgcgcgcaaa tgccagttct gcttcgctga
     1321 tattcattaa acctgcgatg tcacgcgcgt actttccggg attttgttct tttaactcaa
     1381 gccagcgtgt gtagtggttc attgtctctc ttccttccag ataaatgccc cgaaatatcg
     1441 gggcagatga ttaccattga taactcacga aaatttttcc gttacgacca tcctgtggga
     1501 tgccttgcgg cgaccagtac tctttatcga aggcgttgcc caataccaga gtagtggtca
     1561 tgcctttgag cgcctgttgt ccttgataac tgacgtagaa atcattcaca ccatagccag
     1621 gttgtttgct gtagctgctg ctgatatgtg ttgagcgatc ggcaaacgta ccgacccaac
     1681 caacagagaa gccactgtga gcgatcggaa tattcagggt actggtaacg gtatccgggt
     1741 taatgctgga gatatattcc ccggtatcgg tgtctttgcc gcgggtacgg ttataggcca
     1801 catcaaggct aaacagatca gtggtatatt tcgtcatcac atcccagccc cagattttgg
     1861 cgttcgggac gttatacgac atagttgtcg ccgccgcgaa atcgacggtc gtggagatat
     1921 aatctttcgc tttggtatca aagtagctgg ctttaaattc cagagcatca ttggacaaca
     1981 tcaggtcatc aaaacgtagc ccaaaaccgt actcctgagt ttcgttagtt tccggacgta
     2041 agttcgggtt tggcacccaa tagttggtat agaagcgacc aatcgagaag tgtttagaat
     2101 cattatacat ttcgcccatc gtcggggcgc ggaatgcctg agcatatgag ccaaataaca
     2161 tcagccagtt ggtcgggttg atagtcatcc ccgcacgaga tgaccatttg tcggcatcaa
     2221 catctttgta gccgtcgctg ctaccgcgat aactgtcata gcgggttccg ccaagcaggg
     2281 taatcggcag atcgcgtaag gtgatctcat cttgtagcca gccagagcta aaatcgattt
     2341 ttgcttgcgg gaagcccgtc gtcgcgccac ccggatgttg ttcctgacga taatactcac
     2401 cgccatatgt cagtaagtga gaagcgaaac tgtcggcaaa tagagtggaa cggttctcca
     2461 gccttgctcc ttttgttatc tgttcacgat actcgcctga actccccgtg ttttgcgcat
     2521 taatacggac ttccgaccag tatatttttg catctgcatt taaccagtcg ttaccctgcg
     2581 gggcgagttt ataagaaagc tgcgcatcgc gttgaattgt tgaacgatcg accatcgggt
     2641 tgctgctatc agaagcttca acagtctgcg gattttttgg ttcacgcgcg tcgttgttgt
     2701 agtaacgcac taaaccgctc agagactggg ctgaatcaat ttgccaggtc cctttcgcca
     2761 gcatgttatt aatggattcg tcattcggcg cggtttcacc attgctctgg cgtaaatcac
     2821 cccgatcgcg actggaccag gccacaatac catccagatt ttcagttcgc ccaaacgcgc
     2881 tcgcgcctaa tcccaggcta tggtcccccg tgccgccagt accaaagaca cgaaaaccac
     2941 tgctttgtcc ttcctgcaat aaatcttttg catcgaccgt atcgtaggag atcactccac
     3001 ccagcgcgcc actgccatac agtaatgctg aaggtccgcg aacaatctca acacgcttga
     3061 tcagcgccgg atcgagaaaa gtgccattca ggtgtccggt atccgttccc tggcgaacac
     3121 catcgacaag aaccagcacg ccgcgatgat catagccacg catatttaca tcctgaccgt
     3181 tggttcgtcc ggtaccatca agagtaattc caggaacatg acgcagcaaa tcagtggctg
     3241 aagtagcagt ttgattttca ggagcggaag tgtcgataac gctgaccatc ataggcgctt
     3301 cgaaggaact acgtgcattc cccgttgccg taacggtcat ggtttcagta gcaaaagcaa
     3361 acgttggcaa ggtggcagaa acagccaaag ccaacaaact caaacgcaac gaagtaaatt
     3421 gcggacgtga catagcgatt ctccatgagg aaaataaaaa atgctggctg cctgggttaa
     3481 taccggggtg gctggcgaaa aataaattta tttacaattc aaatgattag atatcaccac
     3541 gggaagctat tagcgtatat atcccgtaaa attgccgaat atttctgata attcagcaaa
     3601 agcacgccct ctttgtcagg caaagtagta cgccacgtct gaagtgaaga gtgctgtgcg
     3661 ttttccctta caaaagatat
//
LOCUS       DACADA010000271         3635 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.276, whole genome shotgun sequence.
ACCESSION   DACADA010000271 DACADA010000000
VERSION     DACADA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.276"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            69..3635
                     /locus_tag="ISB17_004668"
                     /pseudo
     CDS             69..3635
                     /locus_tag="ISB17_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1072 a          736 c          890 g          937 t
ORIGIN      
        1 tgggtgatgt aaaagtaact gtttcagcaa tgattgcttg cgctgtttta ttgcacccaa
       61 ataaattgtt tgcctccgtc gctggaacct cacttcccta tcaaacgtat cgtgattttg
      121 ctgagaataa gggagagttc cgtcctggcg cagaaaatgt tcctctatat gataaaaatg
      181 gcaatcccgt cacgacgctg aataaggcac ccatgattga cttcagctct aatgactcga
      241 ctggggttgc aacactggtt tcgccacagt atgtcgtcag cgttaaacac aatgttggtt
      301 atcaatatgt gaaatttggc tatgcagatg attcatccta tgcccttgtt gacagaaata
      361 accactggag agatttccac actccccgtc tcaacaaaat tgtcaccgag gttacacctc
      421 tggatataac caatgcgggt acagctaagg gaacctatca aaatgctgac cgtttccctg
      481 tgttctatcg agtaggtacg ggaactcaat atgtaaaaga tactaacgga aaaatcacct
      541 atttaatggg agcctactcg tataaaacag gtggaatagt aaatacgccg gctatatctg
      601 actggagttt tgtcacgaat accactaata ctccactctc aacctacggc actcccggtg
      661 acagtggctc tcctcttttt gcctgggatg cagaccaaaa taaatgggtt ttgttagccg
      721 ttctcaattc atatgcaggg gtcaatggca atactaactg gtacacaata attcctgctg
      781 gtgatgtaaa aaatacgatg aaacaagatg ccgatgcacc agtaaataca aaacaaggtg
      841 aaggggatat tcattggtca tatgacgaaa aaacgggact tggttcatta acccagggaa
      901 gcacatcatg ggaaatgcat ggaaatttgg gggcaacatg gcctgcttcg cttaatagtg
      961 gtaaagatct aacttttcag ggcggcggta ctgtagtgtt agaaaatacc gttaatcagg
     1021 gggctggcac acttactttt gatgatgatt atattgtcaa gcccgttgat actcagacat
     1081 ggaaaggcgg cggaattatt gttaatggtg aacatcttgt tgactggcag attaatgggg
     1141 ttactggtga tagtttgcat aaactgggta cgggtacgtt aaaaattaac ggtaccggtg
     1201 taaacccagg aagcttaagt gtgggagacg gaaccgttat attggcacag cgtgctgatg
     1261 ataacggact gagccaggcg ttcagttctg tcagtattgt aagtgggcgt cctactctgg
     1321 tgctaaacga cgacaaacaa attaatccgg ataatatcaa atggggctat catggcggca
     1381 agctcgacat taatggaaac agtttgactt ttcataaact taacggggct gatgatggag
     1441 ctatactgac taacagtggc tccatggcta atgttaatct ggatttcaac agcccagata
     1501 caacagccac gattgctaat atctggcacg gccatttcac cggtaatcta aatatcaata
     1561 atgaggttac cgcaggaaca cagaatgatt tcgctattga tggtggagtg aatgctcagg
     1621 ggagtataac ccagcaaaat ggtcggctat ttatgcaagg acatccggtt gttcacgccg
     1681 tcagcagtca ggacgtagca aataagctaa aggcgctggg tgacaattca gtactgaccc
     1741 aaccagtgtc atttactcag aacgattggg aaaatcgaca gttcagtatg gcggagctta
     1801 acttacaaaa tgctgaattt aaccttgcaa gaaacgccag ccttaatacc agaattaatg
     1861 ccgagcattc taccgtgacg ttaggtagtg aggatcttta cattgatctc aatgacggga
     1921 atggcgtggc aaccaaacca acattgggta agtcaaaggc aaccgctgaa gatgatcaga
     1981 gtcgtttcaa tggacatgta cagttgcaac aaggatcggc actaactatt aatgaacatt
     2041 ttgccggcgg tattgatagt gcagatagtg cgacgacgat aacctctaca gataccacgc
     2101 ttaatcagct cagccgtttc acccaatctt ctttgtcatt aggtgaaggg gcaaaattga
     2161 ccgctacagc cggattactt tctgacggga cggtaagcag taatgccggg gcgagccttt
     2221 ccttgctatc agaccagcct ggaacgatgt attccgccca aagctgggag cttagcgggc
     2281 aagacacgag cttaaatgtt ggcgcgggtg ggatcatcac tggagatatc aatgccaatg
     2341 atgcggccag cattagcttt ggcacgactg atattaacca atccacaaat tactatggaa
     2401 atatcaacgc gccgttggca agtgtaacaa tgaaggacac tgcctggcag gtaaacaagc
     2461 agtcggtggc taagtcgctc actttgaatg gttcaacact gagctttaat cgtttcgggc
     2521 aagggggact gacgtcagat acgctggagg ccactaacag ttcgttcatt atcaatgccg
     2581 atggtaaagc agcagatacg gtgacggtaa accaggcatt gacagggggg aataacacac
     2641 tggttgttat tcctacgaca aattcagtaa aacagggcgg tgaccctgtg tcattggtta
     2701 ctgccccgaa aaatacacaa agcaatatat ttacacttaa cccggtttca ataaatgcag
     2761 ggttccatag ttttacaccg caacttgatg tgttggagac cgacgttaat aagcaatggc
     2821 gtctggaggg attttacata cagcctgata aagccgcttt gcggacaggc aagagtttta
     2881 tggatctcgg ttataaaaac tttattacag aaattaataa tctgaatgat cgtatggggg
     2941 acttacgcca tacccatggt gaaactgggg catgggctag acttaacagt ggttcgggct
     3001 cagctactga tggatttacc ggaagttata cgcacttgca aattggggca gacaggaaac
     3061 atataattga aggcggagag ttatttactg gagtaacggc gacatttaca agcagtaata
     3121 accggggaac tggttggtcg ggaagaacca aatcaacggg tattggtgtg tacgcttcag
     3181 ctatgtttga ctctggactc tatgtagata ctatcgggaa atatgtgcgt catgacaatc
     3241 attactcatc aagtgcgtta ggaatgccag aacaagatta tggctcgcac tcctggtatt
     3301 tgggcgctga ggccggttgg cgtttttcct tacctgacga aacatatata cagccgcaaa
     3361 cagagttgat ctacggtact gtatctgaga atcagtttgc ctggcaattc aatgggggtg
     3421 agatctatat gcaacgtaag cagatgcaac cattgattgg tcgcacagga attgaatttg
     3481 gcaaaacatt ccgcggcaaa gactgggaaa tgacagcgct gactgggatt aattatcagt
     3541 acgatttatt taagcctact gtgacagcgt tcaaggatct ggcaggtgat acatatatta
     3601 ataatggtaa agatagcaga gtggtattca atgtc
//
LOCUS       DACADA010000272         3590 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.277, whole genome shotgun sequence.
ACCESSION   DACADA010000272 DACADA010000000
VERSION     DACADA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.277"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            11..505
                     /locus_tag="ISB17_004669"
     CDS             11..505
                     /locus_tag="ISB17_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982511.1"
                     /translation="MLDSDGFIYGQNLRLCTEARYICPVQQIIDTPLLRKLNQFRTFV
                     RNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAEDMNSEQAANI
                     ARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFDATNSELCAE
                     RHEC"
     gene            638..1900
                     /locus_tag="ISB17_004670"
     CDS             638..1900
                     /locus_tag="ISB17_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1982512.1"
                     /translation="MKANFTLSDGDKAVTDADGKAKVTLKGTKAGAHTVTASMVGGKS
                     EQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTRLQATVTDGNGNPVEGIKVNFRGTS
                     VTLSSTSVETDDQVFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKVDVNSATIT
                     SQEIPEGQVMVAQDIAVKAHVNDQFGNPVTHQPATFSAAPSSQMIISQNTVSTNTQGV
                     AEVTMTSERNGSYTVKASLANGASLEKQLEAIDEKLTLTSSPLIGVNAPKGATLTATL
                     TSANGTPVEGQVINFSVTLEGATLSGGKVRTNSSDQAPVVLTSNKVGTYTVTASFHNG
                     VTIQTQTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVY
                     FALKSGSTTLTSLTAVTDQNGIATTNVR"
     gene            2120..2596
                     /locus_tag="ISB17_004671"
     CDS             2120..2596
                     /locus_tag="ISB17_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HAP1982513.1"
                     /translation="MQSGTNVPYMKISAIDYSQNINGDYKATVTGGGEGIATLIPVLN
                     GVHQAGLSTTIEFISAETRPMTGTVSVNSANLPTASFPSQGFTGAYYQLNNDNFAPGK
                     TAADYSFSSSASWVGVDATGKVTFKNDGDSNTVIITAPPRSGGAIYQTVPPESRSV"
     gene            complement(2724..>3590)
                     /locus_tag="ISB17_004672"
     CDS             complement(2724..>3590)
                     /locus_tag="ISB17_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAP1982514.1"
                     /translation="TSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITL
                     QYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQNLRA
                     PVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQERTF
                     SLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRSAAII
                     KDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDE
                     FAKALKSVLARI"
BASE COUNT         1032 a          836 c          824 g          898 t
ORIGIN      
        1 taaactatgc atgcttgaca gtgacggttt tatttatggt caaaatttaa gactctgtac
       61 agaagccaga tatatttgtc ctgtgcaaca aattatagat acccccctgt tacgcaaact
      121 caaccagttt cgtacgtttg ttcgtaatgt ccgcccgggt gatgaactgg atgtccaggc
      181 acaagttagt gaaaaaaatt taaccccgcc gccgggtaac agcagtggca accttgagca
      241 acagatagcc agtacttcac agctaattgg atctctgctc gccgaagata tgaacagcga
      301 gcaagcggca aacatagcac gtggatgggc ctcttctcag gcttcagggg taatgacaga
      361 ctggttaagc cgcttcggta ccgcaagaat cacgctgggc gtggatgaag atttcagcct
      421 gaagaactcc cgtgacggca accctcgaaa atggcgattc gatgcaacaa acagtgaact
      481 atgtgccgaa cgtcacgaat gctgaaatca cgctggcagc ctcgaaggat ccggtgattg
      541 ccgacaataa cgatctcacg acactaacag caccgtcgct gatacagagg gcaatgcgat
      601 agccaacact gaggtaacat ttactctacc ggaagatgtg aaggcgaact tcacgctgag
      661 cgatggcgac aaagcggtta ctgatgccga tggcaaagcg aaagtcacgc tgaaaggtac
      721 aaaagcaggc gctcataccg tgacagcatc gatggttggc ggtaagagtg agcagttggt
      781 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
      841 tttcatcgcc aataacattg gaatgaccag gctacaagca acagtgactg atggaaacgg
      901 caacccagta gaaggtataa aagttaattt ccgtggaacc tccgtcacgc taagcagcac
      961 cagcgttgaa acggatgatc aggtttttgc tgaaattctt gtgacaagca ccgaggtcgg
     1021 actaaaaaca gtttcagcct ctctggcaga taaacctact gaagtcatct cgcgattact
     1081 gaatgccaag gtagatgtta attctgcgac gattaccagt caggagatac ctgaaggtca
     1141 ggtcatggtc gcacaagaca tagcagttaa agctcacgtt aacgaccagt ttggcaaccc
     1201 ggtgacgcat caacccgcga cattcagtgc agcgccatcc tcgcaaatga tcatcagcca
     1261 gaacacggtc tctactaata cgcagggagt agccgaggtc accatgacgt ctgaaagaaa
     1321 cggctcgtat actgtaaaag catccctagc gaatggagct tcacttgaga aacaactgga
     1381 ggctattgat gaaaaactga cactcacgtc cagtccgctt atcggtgtca atgctcctaa
     1441 aggcgctact ctgacggcaa cgctaacctc tgcaaatggc actccagtgg agggtcaggt
     1501 catcaacttt agcgtaacgc tagaaggtgc gacgttaagt ggcggaaaag tgagaaccaa
     1561 ctcttcagat caggctccag tcgttctgac cagcaataaa gtcggtacat atacggtgac
     1621 tgcatcgttc cataacggcg tcacaataca gacacagaca accgtgaagg ttactggcaa
     1681 cccaagcacc gcacatgttg ctagctttat cgctgatccg tcgactatcg ccgccaccaa
     1741 cagtgattta agtaccttaa aggcaacggt tgaggatggc agtggtaacc tgatcgaagg
     1801 tctcactgtg tacttcgcct taaaaagcgg ctccaccaca ttaacgtcat taacagccgt
     1861 gaccgatcaa aacggaatcg cgacaacaaa cgtgagatga gcgataacag ggagcgtcac
     1921 tgtaagcgca gtcataagcg caggtggaat gcaaacagta gatatatcgc tagtggcagg
     1981 cccagcagac gcctcgcagt ccatccttaa gaacaaccag tcatcactga aaggggactt
     2041 taccgatagt gctgagctac atcttgttct gcacgatata tcaggcaatc cgatcaaagt
     2101 ttcttaaggg atagaatttg tgcaatcagg tacaaacgtg ccctatatga aaattagcgc
     2161 aattgattac agtcaaaata tcaacggtga ttacaaagct actgttacag gaggcggaga
     2221 gggtatcgca acgctgatcc ccgtattgaa tggtgttcat caggcgggtc tgagtaccac
     2281 gatagaattc attagcgcgg aaaccagacc aatgacgggt acagtgtcag tgaatagtgc
     2341 aaacctaccg acagcttcat tcccttcgca gggatttacc ggggcgtatt atcagttgaa
     2401 taatgacaac tttgcaccag gaaaaacggc ggctgattat tcgttttcaa gctcggcctc
     2461 ctgggtaggc gttgatgcta ctggtaaagt gacattcaaa aatgacggtg atagcaatac
     2521 tgtaataatc acagcgcccc caagatccgg tggtgctatt taccaaacag ttcctcctga
     2581 atcgcgttcg gtgtgactat gatcattttc ggtgagtatt gctttgatcc aaaggctggt
     2641 tttctgtgat gattgaccag cctcagagta ttgatgacac caccgttcat aacagatcgg
     2701 cattactatg cataagtact gcctcaaatt ctcgccaaaa cactttttaa tgcctttgcg
     2761 aattcatcca gacttatatt gtcaattttt tgcccaacga cgactgactc cgaaacatcg
     2821 gccattgcgg gccatcgcgt tttgtaatca gcataaattg caacaacagg ggtattatag
     2881 gcacttgcca tatgtaatat tgaggtgtca ggagttattg caatataagc atcttttatg
     2941 attgctgctg aacgtttaat cgagggtgat aaggataaac ggtagacatt attataacaa
     3001 tccactaatg ccctggcttt atcttcccct ttgggtccat aaacgatcac tattggaata
     3061 tctgtttcac tttgaatttt tgctattaga ttttctgcaa tcgataatga aaatgtacgt
     3121 tcctgcgagc taccttctaa attgagcgca atgtaagatc ctaacgatcg catttcctcg
     3181 cgtacctcat cgagtacatc atcgcttaat ggcaattcat atattgacct gactgccgga
     3241 aaacccgcct cacgcatcat aaatgcccat gtcatgggta caggagcccg gagattctga
     3301 tccatccggg acgcgttctt acacaaaggg gaatagcact tcatggttaa accgacgacc
     3361 tggaaattcg tttttgcttt caactgactg ataaatatca tcgttttcag gttcttttta
     3421 cgcatcgcct ctatacaaag atccggcgta ccatattgca gtgttatgtt cttcgctaaa
     3481 tcttttattt cttttaatgt tgcgtgatcc tgcatagtca ttgtgactaa tgttaattta
     3541 gtctgttcaa gtttgagcgc attaaagact tctaaattaa ttgtcgacgt
//
LOCUS       DACADA010000273         3565 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.278, whole genome shotgun sequence.
ACCESSION   DACADA010000273 DACADA010000000
VERSION     DACADA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.278"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..178
                     /locus_tag="ISB17_004673"
                     /pseudo
     CDS             1..178
                     /locus_tag="ISB17_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            139..258
                     /locus_tag="ISB17_004674"
     CDS             139..258
                     /locus_tag="ISB17_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP1982515.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            293..635
                     /locus_tag="ISB17_004675"
                     /pseudo
     CDS             293..635
                     /locus_tag="ISB17_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1002..1268)
                     /gene="minE"
                     /locus_tag="ISB17_004676"
     CDS             complement(1002..1268)
                     /gene="minE"
                     /locus_tag="ISB17_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAP1982516.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1272..2084)
                     /gene="minD"
                     /locus_tag="ISB17_004677"
     CDS             complement(1272..2084)
                     /gene="minD"
                     /locus_tag="ISB17_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAP1982517.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2108..2803)
                     /gene="minC"
                     /locus_tag="ISB17_004678"
     CDS             complement(2108..2803)
                     /gene="minC"
                     /locus_tag="ISB17_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAP1982518.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3323..>3565
                     /locus_tag="ISB17_004679"
     CDS             3323..>3565
                     /locus_tag="ISB17_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982519.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFS"
BASE COUNT          903 a          823 c          843 g          996 t
ORIGIN      
        1 gaagatattc attagcaacg attacattag tcattttatt ttgtggacgg cctcattgtc
       61 gaaaaataag cgtacgacag tattatcaga aaagagtgtt ttttatccaa caacacttca
      121 gcgcactgcg tttaaaaaat gcctctttct tatacgggat atcatcattt catcatgatg
      181 tctttgatga gcggtgaaca caatacattt gcgctgtctc ttcaggatga atcccctggt
      241 ctggtgcctg cgggctgatg ttgcagcaga gctaaggtca cttagacgct actatcattt
      301 atccaatgca tggaatcgaa atcagtcgat acccgcagta tatatcgtga actgggcgca
      361 acgctgagct acaacatgcg tctggggaac ggtatggaaa ttgaaccctg gctgaaggcg
      421 gctgtgcgca aagaatttgt cgatgataac cgggtgaagg tgaatagtga cggtaatttt
      481 gtcaatgatt tgtcgggcag acgtggaata taccaggcag gtattaaagc ctcattcagc
      541 agtatgttaa gcgggcatct cggggtgggt ataaccgtgg tgccggtgtg gagtccccgt
      601 ggaatgcggt ggccggtgtg aactggccgt tctgaccatc aacgattaac tgcgtttcgg
      661 cgctgttttc gtttacaggg tgctaaaagg gaaaattctg gggcaaaaaa agcccgccag
      721 ttacggcggg aaacctcatc ctattggaga acaatgaata atgaaattgc gggttatcat
      781 ctccctggat acccatgcta acaataaggt tatttactca accaggcgta aacattttgt
      841 tctgtgcgtg ggaacagcct taaggtgtaa agggggggga aatagcaatg agtagtatca
      901 gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac tcaaggcaga
      961 gatgactctg ccttgaagat aaatgcgctt ttacaggggg cttatttcag ctcttctgct
     1021 tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt
     1081 acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag aatatcttta
     1141 cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc
     1201 tgcaaccgtt cttttgcaat gttggctgtg tttttcttcc gcgagagaaa gaaatcgagt
     1261 aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg
     1321 aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc
     1381 gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat
     1441 tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg
     1501 ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag caggtgctct
     1561 ttaatcggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata
     1621 cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca
     1681 aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat
     1741 tcaaaatcca tcgctttcag atcatcaaga actttggcga ccccttcacg ggtgagggca
     1801 tctttatcgc gagtttgcga tgccggaaga atatagagat tttcagtacg tttatcttta
     1861 attaacgcct ggtttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc
     1921 cggcgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg
     1981 acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta
     2041 ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa
     2101 aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgtgccg
     2161 ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca
     2221 ccagttccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg
     2281 ccagcgcacg accgcgcatc atgccataga catgaatgtt accatcggca atcaattcgg
     2341 cccctgcgct aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac
     2401 cggaacgcac tggggtatca attaaacgcg tttttgtgac cggcgttgta ttttgcgctg
     2461 gagcctgcgg tgcgggggct ggacgcggcg atttttcctt tccttccgtc aggataggca
     2521 gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa
     2581 cccgcaaacc agttgccgaa accgccttat gcatcgccga ccagtttacc gggtcttcca
     2641 gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt
     2701 tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga accacagata
     2761 aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt
     2821 agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc
     2881 tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat
     2941 aaatggcgtt gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac
     3001 ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatcttcag
     3061 acctgtttaa atatccagta ttgaggcgag tgtatttttt caccacgcca ggctttctta
     3121 actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccgc
     3181 agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa
     3241 ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac
     3301 ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg
     3361 tgtggggatg atgttctcat caatggcgtc tggaaatgac gctggcgggc ttcaatctcc
     3421 tgcgtgtggc gttgtttgcg acccgtatat ttgtgtgagt tcagatggca tttccccaga
     3481 gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcat tacaaggcta
     3541 cgatcccagc gaatttacat tctct
//
LOCUS       DACADA010000274         3523 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.279, whole genome shotgun sequence.
ACCESSION   DACADA010000274 DACADA010000000
VERSION     DACADA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.279"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            65..394
                     /locus_tag="ISB17_004680"
                     /pseudo
     CDS             65..394
                     /locus_tag="ISB17_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="ISB17_004681"
     CDS             complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="ISB17_004681"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HAP1982520.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1422..2825)
                     /gene="mmuP"
                     /locus_tag="ISB17_004682"
     CDS             complement(1422..2825)
                     /gene="mmuP"
                     /locus_tag="ISB17_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HAP1982521.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            3033..>3523
                     /locus_tag="ISB17_004683"
     CDS             3033..>3523
                     /locus_tag="ISB17_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HAP1982522.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSS"
BASE COUNT          880 a         1063 c          906 g          674 t
ORIGIN      
        1 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
       61 taccgccaac ctgttcccgg caaccttcag cgacggatac gttgatatct acggctatca
      121 tctgccgcgc ccgctgcact ttggtacaca gggtgaaggg atggtcggtg tgcgcccgga
      181 agcgatcacg ctcagcgatc gcggcgaaga gagccagcgc tgcgtgatcc gccatgtcgc
      241 ctatatgggg ccgcagtatg aagtgacggt ggaatggcac gggcaggaga tattattgca
      301 ggtcaacgct acgcgtctgc aaccggacgt cggcgagcag tattatcttg aaatccatcc
      361 gtacggcatg tttgttctgg cggatgcggc ataacgtttt gtggttttgt aggccggata
      421 aggcgcttgt gccgccatcc ggcaatgcct gatgcgctgc gcttatcagg cctacaatga
      481 acgatgtaga cccgataaac cctcagcttc gcgcttttaa cgcggcgata tccgcaggcg
      541 tggtgcgaca gcacccgcca atcaaccgtg cgccagcggc ctgccactgc ggcagataat
      601 ccgccagctg cgcgcaatgt tcaccgtgat gatgccaggt tttgctcacg gcatcgtaat
      661 gctcgcccga gttcggatac accaccagcg gcagcaccgt taaaccgtgt aaatgctgca
      721 acgcagcggt ggtgttttcc agcgcaatac agttaatgcc tagcgccacc acctgcggat
      781 aacccgccag caacgcaacc acgtcacgca gcggcgtacc gtcgctcagg tgttcgctgt
      841 cgcgcagggt aaatgagaac cacgcccgcg cacgcggata tgcggtcaac agctcggcca
      901 acgcctcaat ctcggaaaaa ttcggcaggg tttcgcaggc cagcagatcg gccccggcat
      961 ccagcaaggc ttccacgcgc gggcgatgaa acgcctgaaa tgcctcaacg ctacaatgat
     1021 aatcgccacg gtattcagag ccatccgcca gatacgcgcc gtaaggcccg acggatcccg
     1081 ccaccagaag cgttcccgcc tgcgggttct ctgccagata cgcttcacgg gctttacgcg
     1141 ccagctccac gcttttgcca atcagcgctt tcgactgcgc ttcatccaga cctcgcgcgg
     1201 cgaagcccgc cggcgtcgcc tgatagctgg cagtgatcgc gcattgcgcc cccgcccggt
     1261 agtaatcaag atgcacttcg cggataagct ccgggttttc taccagcact ttggctgacc
     1321 acaggctgtc ggctaagtta caccctcgcg cttccagctc cgtcgccatc gcgccatcca
     1381 gcagcaggat atcctgttta tcaagaagag cgcgtaacgg attattctgc gacatgttct
     1441 ggctcctgtt ttgcgtttcg gggttgagta aggaaataag caccatagca caacgcaaca
     1501 aacggtaacc cgcaccacaa cgcaattctc tgcgctggat cgaatgccag cccaacacag
     1561 gccaccaggc acagcacaaa acctaatact ggcaccagcg gataccacgg cgcgcgataa
     1621 tgtaattcac tcaatgcctt accttgttgc agatgacggc gacgaaaaac aaaatgcgag
     1681 gcgcagatac tcagccacac cgctaccacc gcaaacccgg agattgccga cagcgcaaca
     1741 aataccgtgt ccggggccac cacgctggaa aacagcgcca gcacaccacc gagcatactg
     1801 accgacagcg ccgtcagtgg cacgccgttt ttcgttactc gcgcaaaaca ggccggtagc
     1861 gtacgttcat tcgacaacga ccacagcatg cgcccggagg catataaccc ggagttcgct
     1921 gcagaaagaa tagccgtcag gatcacgaag ttaaaaatat cagcggcgta cgggatccct
     1981 actttctcaa ataccagcac aaacgggctt ttctccacgc ccgcctgctg catcgggatc
     2041 agcgctgcca gcacaaacac ggtgccgata aagaaaataa tcagtcgcgc gatggtagta
     2101 cgaatcgcta ccgggataac tttgcgcggg ttttccgttt caccggcggc aatgccgata
     2161 agctcggtac ccgaaaaagc aaagttcact gccaccatag tcatcaaaat cggtaagcca
     2221 ccgtgcggga accagccttc tgccgtgata ttactcagcc ccggcgcggg cgagccatcc
     2281 tgcatcggaa taaagccgaa aatcgccgcc ccaccgagga tgataaaggc gatgatagtg
     2341 accactttga ccagcgagaa ccagaactcc ccttcggcaa aaaagcgcgt ggagataaca
     2401 ttcagaccaa aaataatcgc gcagaacacc acgcaccaga cccataccgg cacctgtgga
     2461 aaccagtact gcatacagaa tccagcggcg gtaaagctcg aacccagcgc cacggtccag
     2521 gtcagccagt aaagccaggc cacggtatac ccggtagccg gaccaagata gcgcgcggca
     2581 taaacgtgaa acgctccggt ctccggcatc gcgaccgaca gctcgcccag acactgcata
     2641 accagccaga ccaccagcgc accaatcaga taggccagca gcgttcccgc cgctccagtg
     2701 gtggaaatga tgtacccggt attgaagaat aatcctgtgc caatcacgcc gcccaaggaa
     2761 agcataatca ggtgacgcgt tttcattgtg cgcttcagtg gcgcattttg ttgtgttgtt
     2821 tgcatataac cttctaaacg tatggacatc taaacatcca aagaaggttt gttataccgc
     2881 gtttgttaac agaatgcaaa gtcaaccctg caccagacgc agatgcccgc gccgggcgct
     2941 taatagcggg tggggattaa taatcagata caaaaagggc cgccagctca taacgcctcc
     3001 ggaaggtgcg aacaagtccc tgatatgaga tcatgtttgt catctggagc catagaacag
     3061 ggttcatcat gagtcatcaa cttaccttcg ccgacagtga attcagcagt aagcgccgtc
     3121 agaccagaaa agagattttc ttgtcccgca tggagcagat tctgccatgg caaaacatgg
     3181 tggaagtcat cgagccgttt taccccaagg ctggtaatgg ccggcgacct tatccgctgg
     3241 aaaccatgct acgcattcac tgcatgcagc attggtacaa cctgagcgat ggcgcgatgg
     3301 aagatgctct gtacgaaatc gcctccatgc gtctgtttgc ccggttatcc ctggatagcg
     3361 ccttgccgga ccgcaccacc atcatgaatt tccgccacct gctggagcag catcaactgg
     3421 cccgccaatt gttcaagacc atcagtcgct ggctggccga agcaggcgtc atgatgactc
     3481 aaggcacctt ggtcgatgcc accatcattg aggcacccag ctc
//
LOCUS       DACADA010000275         3430 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.280, whole genome shotgun sequence.
ACCESSION   DACADA010000275 DACADA010000000
VERSION     DACADA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.280"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            246..482
                     /locus_tag="ISB17_004684"
     CDS             246..482
                     /locus_tag="ISB17_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1982523.1"
                     /translation="MVKLALQPRDSVACIAREHDINDNLLFKWLRLWLIKGRISRRLP
                     VTNSSGIGVELLPVEMTLDERPYPVFTRGNYEDQ"
     gene            complement(682..1557)
                     /locus_tag="ISB17_004685"
                     /pseudo
     CDS             complement(682..1557)
                     /locus_tag="ISB17_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1836..2086)
                     /locus_tag="ISB17_004686"
                     /pseudo
     CDS             complement(1836..2086)
                     /locus_tag="ISB17_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2174..3418)
                     /locus_tag="ISB17_004687"
     CDS             complement(2174..3418)
                     /locus_tag="ISB17_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982524.1"
                     /translation="MLQIKARTKILALFCAMSFLMYVDRVNLSATAGLIKDELGLSNT
                     ELGVIFSAFAYTYAVFQIIGGWFVDRLGSRRMIIFCGIIWVIATMATGFVGGFISLFL
                     ARLLLGVGEGAALPSQARAITYWFKPQQRGFVQGVTHSFSRLGNAITPPIIVALVAFW
                     SWRGAFISLAIVTFIWLISWIISYKDDPRDHSGMTEEELEGVPIFEKKDLKVKAEPTP
                     WRALIKRMGPTMGVYFCYGWTSWLFFTWLPTFFMHGRGMDLKSSALFTAGVFLSGVVG
                     NTVGGVISDKVLKMTGNLVAARRNVILFSFISVLFLLVPVINTDSLLIMTICLSVAFF
                     CLELTIGPIWAVPMDITPKYVGIASGLMNAGSAVAGIISPIVFGIIIDKTGNWSLPFY
                     GSVALLIIGIFLTFFMRPDKSL"
BASE COUNT         1029 a          824 c          738 g          839 t
ORIGIN      
        1 tggtcacaat ttcagatttt ttccagtatc tattttccga ttcgttcggc ggtaatccac
       61 cgttatattc atgaggccgg agtgcactat aaaagtgtat gcgtaaaccg actgctgtat
      121 gtagccatta agcctgtatt ggtaacatag gtacccacct tttcaacaag tggacacctg
      181 ttatagaaca aaaaacatta tcagcagaac cccgcaggtc attttcaaat gagtttaaac
      241 ttcaaatggt taaactggct ttacaaccaa gggattctgt tgcctgtatt gcccgggagc
      301 acgatatcaa tgataatctt ctgttcaaat ggctcaggct ctggctgatc aaagggcgta
      361 tatcacggcg tcttccggta acgaactctt ctggcattgg cgttgaatta ttacctgtgg
      421 agatgacgct ggatgagcgg ccttaccccg tttttacgcg aggtaattat gaagatcaat
      481 gactagccgt cgaaccgtgt gcatgaactg ctgtactgaa acttctcagc tgtaaaataa
      541 tccttacgct acgtacttct cggagcgctt acgattaatc agatgcagtc ctaactggaa
      601 gaaagaaaaa tggagaaaat aagaggaatt aatatcctcc acgttcctga aatcctggag
      661 gatattaatg tcatcaaaat attagcgaac gataatccct aacaaaatac ccagtgcgcc
      721 aaagtcagca tcactgaagg tggtgttagc aaaaccaata tcacccagca caggcaataa
      781 gaatactgac aggaaggtaa taagcaagcc ttgcgcaaag gcacccaaaa tcgcaccacg
      841 acgtccaccc gttgcattac cgaatacccc tgcagctgca cctacgaaga agtgcggcac
      901 cacaccagga ataatcacga tcatgttcag cagatacaac gtgaacatac cgattaaacc
      961 tgcagcaaag ctactcagga aaccaaccag tacagcgtta ggtgcgtaag ggaagatgac
     1021 cgggcaatcg agagcaggtc tggcattcgg aaccagcttg tctgaaatac ctttgaatgc
     1081 cgggacaatc tccgcaatca ccatgcgcac accctgcaga atgatgtaca cgccagctgc
     1141 gaaggtaatg gattgcatga tagagaacat gaaccagttt ttaccaccgc taagctcttt
     1201 caccgcatct gcacccgcaa acaggcacgt cagcaggaaa attatgatca tggtaaaaga
     1261 gatagcaacc ggcgtgtcgc gcaagaacag cagacttttc ggcacattca tgtcttcagt
     1321 cgagtgctct ttattaccaa acttgctgcc gatgaaaccg gccaatacat aagataatgt
     1381 tgagaagtgg ccgatggcaa cgtcatcaga accggtcact ttcttcatgt acggatgagc
     1441 aatagccggg aaaaagacca ttgccacgcc aacaaccaaa gagccaacgg ctataagcgt
     1501 aatgccggtc atgcctgctg ttgccagaat taccgctacc atcatcgaca taaacaacgc
     1561 ccgcagcatg agaatggaca atagacattc gggaggattt tttcatataa atctccttgc
     1621 tcatttttaa tgaacgaagg aggagacgac ggagaaatca ccttcctgaa cgggatcaag
     1681 ctaccactgt cggatccgcc acactccctg aaccatgttt tttaagggtc ataccaccaa
     1741 aaggtcgacg gctgcttcct tcgctatatt agactgccag ttttaccggg gagtttctga
     1801 gactcgggac ggggtttcac acatggaaag ttttttaata tttcgttttc catttcaatt
     1861 cgttgtagct ttttctttag ctcacgtagt ttgatttgtt ccggtgttat cagggaggct
     1921 tttggcgttt accctgacgc tcatcacgca gttgtttgaa ccattccgtc atcgtggaaa
     1981 gaccaacatc catagcctta gcagcgtcag aaacagtgta attttgatcg acaaccaact
     2041 gagcggattc acgtttgaat tcaggactga aatttcttct tttcataggt gcacctgtgt
     2101 tgttctaagg tgagcatatc accctgttca gatggccaaa ttcagtagac cacttcagcc
     2161 ctgaattcga cttttacaat gacttatctg gacgcatgaa gaacgtcaga aatataccaa
     2221 taataagcaa tgcgacggaa ccgtagaatg gtaaactcca gttgcctgtt ttgtcgataa
     2281 tgatgccaaa gacgataggt gaaataatgc cggcgacagc ggaaccggcg ttcatcaatc
     2341 cgctggcaat tcccacatac ttcggcgtaa tatccatcgg gacggcccag ataggcccta
     2401 tggtcaactc aagacagaag aacgccacac tcagacatat agtcataatg agcaacgagt
     2461 cagtatttat aaccgggacg agcagaaata gtacggagat aaagctgaaa agaatgacgt
     2521 tacgccgggc tgctactaga ttgccagtca tcttcagcac tttgtcgctg attacgccac
     2581 caaccgtatt acctactaca ccggacagaa atacgccggc agtaaagagc gccgatgact
     2641 taagatccat gccgcgacca tgcataaaga aagttggtaa ccaagtaaaa aacaaccaac
     2701 ttgtccatcc gtaacagaaa taaactccca ttgtcggtcc cattcgtttt atcagggctc
     2761 tccatggagt aggctcagct ttaactttta gatctttttt ctcgaaaatt ggtacgcctt
     2821 ctagttcttc ttcggtcata ccgctgtggt ctcgtgggtc atctttatac gatatgatcc
     2881 aagatattaa ccagataaat gtgacaatag ccaaactgat aaatgcgcca cgccaagacc
     2941 aaaatgccac taatgcaaca ataatggggg gagtaatcgc atttcccaaa cgagagaatg
     3001 agtgcgtaac gccctgaaca aagccacgct gctgcggctt gaaccagtag gtgatcgctc
     3061 gcgcctgaga aggcagcgcc gcaccttccc ctacacctaa tagcaatctg gcgaggaata
     3121 gtgatataaa gccaccgacg aaaccagtcg ccatggttgc tataacccaa ataatgccac
     3181 aaaaaatgat catgcggcgt gagccaagtc gatcaacgaa ccagccaccg atgatttgaa
     3241 aaactgcata ggtataagca aaagcagaaa aaataacgcc gagttctgta ttactcagtc
     3301 cgagttcatc tttaatcaac cccgcagtag ctgataagtt tactcggtcc acatacatta
     3361 ggaatgacat tgcgcaaaat agcgcaagta tcttggttct ggcttttatt tgtaacattt
     3421 ttgtctccta
//
LOCUS       DACADA010000276         3367 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.281, whole genome shotgun sequence.
ACCESSION   DACADA010000276 DACADA010000000
VERSION     DACADA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.281"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            288..1604
                     /gene="shiA"
                     /locus_tag="ISB17_004688"
     CDS             288..1604
                     /gene="shiA"
                     /locus_tag="ISB17_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HAP1982525.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1706..3160
                     /gene="amn"
                     /locus_tag="ISB17_004689"
     CDS             1706..3160
                     /gene="amn"
                     /locus_tag="ISB17_004689"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAP1982526.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          791 a          811 c          806 g          959 t
ORIGIN      
        1 aatgcaatgc agtctatcag aatcatcagg gtgcaatttt gtgcatatgc ggacttttat
       61 gtttcatatc tcgaacctgt gggccctctg cttaatcctt aaacaacacc agcaactcct
      121 gcgctttcat cttccatcga atttttcatg ttgccgctaa tcagccataa aatcatttgc
      181 agatgcgctc tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt
      241 taccttttcc atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc
      301 tcatctccac tcgtcccgat gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt
      361 taggcagttt cgctggtgcc gtcgtcgact ggtatgattt tctactctat ggcattaccg
      421 ccgcactggt gtttaatcgc gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg
      481 ccgcatttgc cacctttggt gtcggcttcc ttttccgacc gctcggcggt gtcattttcg
      541 gtcactttgg cgaccgactg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg
      601 gcatcgcgac agccttaatt ggtattcttc cttcattctc gaccattggg tggtgggcac
      661 ctattttgct ggtgacactg cgtgccattc agggatttgc cgtcggcggc gaatggggag
      721 gcgcagcgtt gctttccgtt gaaagtgcgc cgaaaaataa aaaagccttt tacagtagcg
      781 gtgtacaagt tggctacggt gtaggtttac tgctttcaac cggactggtt tcattgatca
      841 gtatgatgac gactgacgaa cagtttttaa gctggggctg gcgcattcct ttcctgttta
      901 gcatcgtact ggtactggga gcattgtggg tgcgcaatgg catggaggag tccgcggaat
      961 ttgaacaaca gcaacataat caagcggccg cgaaaaaacg catcccggtt atcgaagctc
     1021 tgttacgaca tcccggtgct ttcctgaaga ttattgcact acgactgtgc gagttgctga
     1081 cgatgtacat tgttactgcc tttgcactta attattcaac ccagaatatg gggttaccgc
     1141 gcgaactttt ccttaatatt ggtttgctgg taggtggatt aagctgcctg acaattccct
     1201 gttttgcctg gcttgccgat cgttttggtc ggcgcagggt ttatattaca ggcgcgttga
     1261 tcggaacgtt gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga
     1321 tagttttctt ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac
     1381 aaccgatgtt taccgaaatg tttggtgcca gttatcgcta cagtggtgct ggagtcggtt
     1441 atcaggttgc cagtgtggtt ggcggtggat ttacaccttt tattgccgct gcactcatca
     1501 cttactttgc cgggaactgg catagcgtcg ccatttattt gctggctgga tgcctgattt
     1561 ctgcaatgac cgctttattg atgaaagaca atcaacgctc gtgatagcct ggcgaagatc
     1621 atccgatctt cgccttatac ttttgtttca cattcctgtg acatactatc ggatgtgcgg
     1681 taattgtatg gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca
     1741 ggcactggat aaactcgacg cgctgtatga gcaatctgta gttgctttac gcaacgccat
     1801 tggcaaatat attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt
     1861 tgtctatcca tcactgaccg taacctggga cggtagcaca actaatcccc ccaaaacgcg
     1921 cgcatttggt cgttttaccc acgcaggcag ctacaccacc acgattactc gccctactct
     1981 ctttcgttcg tatctcaatg aacaacttac gttgctgtat caggattatg gtgcgcatat
     2041 ctcggtgcaa ccctcgcagc atgaaatccc ttatccttat gtcatcgatg gctctgaatt
     2101 gacacttgat cgctcaatga gcgctgggtt aactcgctac ttcccgacaa cagaactggc
     2161 gcaaattggc gatgaaactg cagacggcat ttatcatcca acggaattct ccccgctatc
     2221 gcattttgat gctcgccggg ttgatttttc cctcgcacgg ttgcgccatt ataccggtac
     2281 gccagtagaa cattttcaac cgttcgtctt gtttaccaac tacacacgtt atgtggatga
     2341 attcgttcgt tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc
     2401 ttgtgctggc gggaactgga tcactgccga aaccgaagcg ccagaagaag ccatttccga
     2461 ccttgcatgg aaaaaacacc agatgccagc atggcattta attaccgctg atggtcaggg
     2521 tattactctg gtgaatattg gcgtgggacc gtccaatgct aaaaccatct gcgatcatct
     2581 ggcagtgcta cgcccggatg tctggttgat gattggtcac tgtggcggat tacgtgaaag
     2641 ccaggccatt ggagattatg tccttgcaca cgcttattta cgcgatgacc acgttcttga
     2701 tgcggttctg ccgcccgata ttcctattcc gagcattgct gaagtgcaac gtgcgcttta
     2761 tgacgctacc aagttagtca gcggcaggcc tggtgaagaa gtcaaacagc ggttacgtac
     2821 tggcacggtg gtaaccacag atgacaggaa ctgggagtta cgttactcag cttctgcact
     2881 tcgttttaac ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca
     2941 aggatatcgt ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttaca
     3001 tggtgagatt aagcttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca
     3061 cctgcaaatt ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc
     3121 acgtaaatta cgaaccttta atgagccgcc gttccgataa taagttatga accttcttat
     3181 cagaaataga tgtgaaggat taagtaagac atctggcaaa tagcctgcaa taaacgcggg
     3241 ctgtttgctt ccttgggcag atgcgagttt tattatcgtc ttaatgattt ccacatatta
     3301 aaagcaagta tgctttcaaa acacaattat aaaaaatccc gccaacaaca ttagttttta
     3361 taaaatt
//
LOCUS       DACADA010000277         3333 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.283, whole genome shotgun sequence.
ACCESSION   DACADA010000277 DACADA010000000
VERSION     DACADA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.283"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            84..800
                     /gene="nanC"
                     /locus_tag="ISB17_004690"
     CDS             84..800
                     /gene="nanC"
                     /locus_tag="ISB17_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAP1982527.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFVWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            820..1926
                     /locus_tag="ISB17_004691"
     CDS             820..1926
                     /locus_tag="ISB17_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAP1982528.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            1990..2970
                     /locus_tag="ISB17_004692"
     CDS             1990..2970
                     /locus_tag="ISB17_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAP1982529.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
BASE COUNT          969 a          718 c          788 g          858 t
ORIGIN      
        1 gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
       61 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
      121 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
      181 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
      241 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
      301 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
      361 cggtacgccc gggaatgtta acgcatttta gcagcaacgg cacacgttac ggaccctacg
      421 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
      481 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
      541 gggatggata tgtcacttac catattaata gtgatttcac cttcgtatgg caaacgacgc
      601 tatacagcaa acagaacgat tatcgctatg caaaccataa gaaatgggcg acggaaaatg
      661 catttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
      721 ttgaccgtca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
      781 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac aataacggcg
      841 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
      901 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
      961 gcaggtacgg catggtacaa gctggataca caggccaaag ataaaaaatg gacagcgtta
     1021 gctgcattcc ctggtggacc aagagatcaa gcaacctcgg catttattga tggtgatctg
     1081 tatgtgtttg gcggcattgg caaaaatagc aagggcttga ctcaggtatt taatgacgta
     1141 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccaatgggc
     1201 atggcgggcc atgtgacttt tgtacacaac ggcaaagctt atgttactgg cggtgttaac
     1261 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     1321 atagataaaa tcaacgctca ctattttgac aaaaaagcag aagattattt cttcaataag
     1381 tttctgttgt cttttgatcc ctcaacccag caatggagtt acgctggcga atctccctgg
     1441 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     1501 gaagccaaac ccggattgcg aacggatgcc gtatttgaac ttgatttcac cggtgataat
     1561 ttaaaatgga ataggcttgc tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     1621 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     1681 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     1741 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     1801 tacggagtat cattgccctg gaataatagt ctattgatta ttggcggtga atctgcaggc
     1861 ggcaaagcgg tgacggattc agttttgatc tctgtgaagg ataataaagt cacagtacaa
     1921 aactaacgct tcagggcccc ggtaaagggc cctgctatca atttgtcatt tcaattacgc
     1981 gaaaattata tgaacgcaat aatatcgccc aattattact atgttcttac cgttgctggt
     2041 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     2101 catcccagaa tcaaacaatt agcgagattt acgcatacgc atcccggagg cccgccatgt
     2161 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     2221 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     2281 catattgcac ggaaattact gcccttcatt cctgataatg cagggattct catcgtcccg
     2341 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgagg ggacatattc agaacggcac
     2401 ggagccagcc atgatgcttg tcgttgggga acggataccc cgctctacca ggatttagtc
     2461 agcagaacgc gagccgcact ggcaaaaaat ccgcataaca aattcctcgg cgtatgctgg
     2521 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     2581 catatggttg aagcctttcg tagggatcta aaacaattcc attctcagct taataatatt
     2641 actgccgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttccctcat
     2701 gcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     2761 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga tccggacgat
     2821 ttaagcacgg gatatttcgg ttcagcgtac cggtcagcgg agaactggac gacgtcactg
     2881 cgaagcagtc atttcagcgc ggcagcccgt cgggggatta tttctgacag atttgtagaa
     2941 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     3001 gtccattccc agagcagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg
     3061 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     3121 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     3181 cttttcggtt atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg
     3241 ccacagcgtt cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc
     3301 tggcaaatag acgatgatga tatgcttaat gat
//
LOCUS       DACADA010000278         3248 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.284, whole genome shotgun sequence.
ACCESSION   DACADA010000278 DACADA010000000
VERSION     DACADA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.284"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..915
                     /locus_tag="ISB17_004693"
     CDS             <1..915
                     /locus_tag="ISB17_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HAP1982530.1"
                     /translation="DHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQL
                     NLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELN
                     SLSDKIQKLSDDAATANADPADMAAQVALITSRINDLTASVILMHAPKGVAVASGEHL
                     QLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDL
                     MELLAKKSIEIVSTEDEIRISAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYG
                     RMGKAHEPVELQMLAEKVDEPPVKFFFS"
     gene            927..>3248
                     /locus_tag="ISB17_004694"
     CDS             927..>3248
                     /locus_tag="ISB17_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HAP1982531.1"
                     /translation="MIISPPFIRAKNNNENDAHWIERMMPVEPDRDYPINYGGSWHGG
                     IHVRHTNSDRQPEYVRAIADGVVVSIRNPSDQAACSLPPLNYNGSTDDGYVLLRHETE
                     IGTGEDGNIVYYSLYMHLEKIEEGISEGNKVYRKSSLGMPGHVDGDRGLHFQIFCDDD
                     NLRKITGRTTPEMDVSRHGRTDVVYGDIYFYLPPGTRFYRSAPENNAVDNTGEVFQST
                     EPLVVTMCFDTGHCIMTTRRANPVIPSRYDIVGEVLTDADGTDYEYNLYSAALRLYPQ
                     SPSAGFELLRFGRVINTEHETLIPANAPLWRTVSYPGGQGVINLAADVVTKFSDGDFP
                     HWSGWRLVDDDADSNSQCNSVILRGGGFTDFRQMICHFPLEWCNTGIEARYGWLKEGV
                     IQERQENEANSDLSLGTDYYRPNNVEYNTTGDSHGESPQSAISEMPDLSYEQSNAYEN
                     AVRVLLLGEEDWQTLVKHIKALCFDTSSIAEGRVWHFNPGEFIKHFRKCGWVDKAILK
                     RIFASGTNDRDRAVIEQKVDIYGTAINIVMNKYIMNSPLRRAHFLGQGAVESSRLRSM
                     QEKSQYQTVDENGRPVGGGIVPDSLRDENSDLGHWYGAIETEVDGYFSGVKYNSGGGR
                     IAGSYDWILGNCDTEDAQKFRGRGFKQLTGRSNYAEYWVYRAWIDTNSFTAKWWEDPL
                     WRLHDRRRLTRIPANIEEPHRVTRSEYNCIDTGGFFIVKTVDRRGTRSSITRAMDQDS
                     EVIHLNNTTIDTTDEQTIRSVSRAINGGENGLPD"
BASE COUNT          916 a          641 c          910 g          781 t
ORIGIN      
        1 gaccatgtga ccattcagaa ctacaagcgt aacgtgctgc gcaccccggc gaacaacaaa
       61 atccgcctcg acgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc
      121 aagagccagc taaacctcgg ccatctggtg gatgccggaa aacagcagcg cggggaaggt
      181 tttgaactgc gtaccgatct gtggggtgca gtacgggcaa agaaaggcat ctttatcagc
      241 gccgacgcgc aggataaggc gcagggtcag gtacgcgaaa tggccgacat tatcagcgaa
      301 ctgaacagcc tgagcgataa aatccagaaa ctgtctgacg atgcggccac ggcgaacgcc
      361 gatccggcgg atatggccgc gcaggtggcg ctgataacca gccggataaa tgacctgacc
      421 gcctcggtga ttttgatgca cgcgccgaaa ggtgtggcgg tcgccagcgg cgagcacctg
      481 caactggccg cagtgaaaaa cctccagata aatgcgggaa acaacgccga tatcggcgtg
      541 gtgaagaata tgtttatcgg cgtagggcgg gcgctgagcg tgtttgtgcg caaggcgggt
      601 atcaaactga ttgccaataa aggcgcagta tcggtacagg cgcagcacga tctgatggag
      661 ttactggcga agaagtcgat agagatcgtc agtactgagg acgagatcag gatttcagcg
      721 aagaaaaaaa taacgattaa tggtggtgga agttatatcc ggattgaggg tagcggcatt
      781 gagccgggaa cgccggggga ttataacgtc aaagcggtgc attacgggcg tatgggaaaa
      841 gcacatgagc ctgttgagtt acaaatgttg gcagagaaag tagatgagcc acccgtaaaa
      901 tttttctttt cttaaggata taagaaatga ttattagccc gccatttata cgagcgaaaa
      961 ataacaatga aaacgatgct cattggattg aaaggatgat gccagttgaa cctgaccgtg
     1021 actatcccat caactatggg ggatcatggc atggaggcat ccacgttaga catacaaaca
     1081 gtgacaggca accagaatat gtccgtgcca ttgctgatgg tgtcgtggta tctattcgca
     1141 atccctctga ccaggcagca tgtagtcttc cgccgctcaa ttataatgga agcacagacg
     1201 atgggtatgt tctgcttcgt catgaaactg aaataggcac tggtgaggac ggcaatattg
     1261 tttattactc tctttacatg cacctggaaa agattgaaga gggtatcagt gaaggaaata
     1321 aagtctaccg gaaatcgtca ctgggaatgc cggggcatgt cgacggggat cgcggcctgc
     1381 acttccaaat tttctgcgat gatgacaatc tgagaaaaat aaccgggcgc actacacctg
     1441 agatggatgt gagccgtcat gggcgtaccg acgtggttta cggggatatc tatttttacc
     1501 tgccgcccgg tacccgcttt tacaggagtg cgccggaaaa caacgcagta gataacacag
     1561 gtgaagtttt tcagagcacg gaaccgttgg ttgtcaccat gtgttttgat actggccatt
     1621 gtatcatgac tacccgcaga gcaaatccgg tgataccaag ccgttatgac attgtgggag
     1681 aagtgttgac ggatgctgac ggcactgatt atgaatacaa cttgtacagt gctgcactga
     1741 gactttatcc gcaaagtcca agcgcagggt ttgagttact gcgctttggt cgggttatca
     1801 ataccgagca tgaaacactg atacccgcga atgcgccact gtggcgaacc gtgagctatc
     1861 ccggtgggca aggagtgata aatctggcgg cagatgttgt gacaaaattt agcgacggtg
     1921 attttccgca ctggagtggc tggcgactgg tagatgacga cgctgacagc aacagtcagt
     1981 gcaattcagt aatattacgt ggtggtggtt ttacggattt tcgtcagatg atttgccact
     2041 tcccactgga gtggtgtaat acgggaatag aagcgcgtta cggctggttg aaagaagggg
     2101 taatacagga acgtcaggaa aatgaggcta acagcgattt atcattaggt acggattact
     2161 acaggccaaa taatgtcgaa tataatacga ctggagatag ccatggagaa tctcctcagt
     2221 cagctatatc agaaatgcca gatttgtctt atgaacagtc aaatgcttat gaaaatgccg
     2281 tcagagtttt attactggga gaagaagact ggcagacgtt ggtgaagcat attaaggcac
     2341 tgtgttttga taccagtagt attgctgaag gacgggtttg gcattttaat ccgggtgagt
     2401 ttattaagca ttttaggaag tgtgggtggg ttgacaaagc aatattgaag cgaatttttg
     2461 cctctggtac taatgaccgt gatagagcgg ttattgaaca aaaagttgat atatatggta
     2521 cggctataaa tattgttatg aataaatata taatgaattc tcctctgaga cgtgctcact
     2581 ttctggggca aggggcagtg gaatcttccc gtttaagaag tatgcaggaa aaaagccagt
     2641 atcaaactgt tgatgaaaat ggtcggccag tcggcggagg tattgttccg gactcattac
     2701 gtgatgagaa ttcggacttg ggtcattggt atggtgcaat tgagactgag gtcgatggat
     2761 acttctctgg tgttaaatat aattctggtg gaggacgaat tgcaggctca tatgactgga
     2821 tactaggaaa ctgcgacact gaggatgccc agaagtttcg tgggcgaggt tttaagcagt
     2881 taactggtcg atcaaattat gccgaatatt gggtctatcg tgcctggata gatacgaata
     2941 gttttaccgc taagtggtgg gaagaccctc tgtggcgctt acatgatcgt cgtcgtttaa
     3001 ctcgcatacc ggctaatatc gaagagcctc acagggttac cagaagcgaa tataactgta
     3061 tcgatactgg tggattcttt attgttaaga ccgttgatcg tcggggtact cggtcaagta
     3121 ttaccagagc gatggatcaa gacagtgaag ttattcattt gaacaatacc acgatagata
     3181 caacggatga acaaacaata agaagtgtgt cccgtgcaat taatggtggt gaaaatggtt
     3241 tgccagac
//
LOCUS       DACADA010000279         3210 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.286, whole genome shotgun sequence.
ACCESSION   DACADA010000279 DACADA010000000
VERSION     DACADA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.286"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(43..570)
                     /gene="mobB"
                     /locus_tag="ISB17_004695"
     CDS             complement(43..570)
                     /gene="mobB"
                     /locus_tag="ISB17_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAP1982532.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(552..1136)
                     /gene="mobA"
                     /locus_tag="ISB17_004696"
     CDS             complement(552..1136)
                     /gene="mobA"
                     /locus_tag="ISB17_004696"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAP1982533.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1206..1475
                     /locus_tag="ISB17_004697"
     CDS             1206..1475
                     /locus_tag="ISB17_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAP1982534.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1552..2538
                     /gene="srkA"
                     /locus_tag="ISB17_004698"
     CDS             1552..2538
                     /gene="srkA"
                     /locus_tag="ISB17_004698"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAP1982535.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2555..3181
                     /gene="dsbA"
                     /locus_tag="ISB17_004699"
     CDS             2555..3181
                     /gene="dsbA"
                     /locus_tag="ISB17_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAP1982536.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          814 a          780 c          783 g          833 t
ORIGIN      
        1 gccggatgcg acgctggcgc gtttgcttca gcctattctt gcttatccgt cctgcttttg
       61 catccactcc accacaaaat cagccagccc ctcaacatca ttaatatcca gtaacgcgac
      121 atcaagatta agcggcacat cactggctac agcaataaca tgcctgtcta tcactaattc
      181 ttccggtcga tgtccggctc catcgcgaaa cagcacaatc tttgcgatct cttcatgctt
      241 aaacccttcg accagaatca aatccagctt tgaggtatcc attcgacttg cgagaaaatg
      301 tagatccagc tcttcttcgt ctggtgtttc cgtcatcaag gcccatcgct gctggctggc
      361 aacgatggtt tgcgccgcgc cagccttgcg cagctcatag ctatctttgc ccggtttatc
      421 aacatccata tcatggtgcg tatgcttaat cagccccgga cggatccctc tggcgcataa
      481 tgccgggatc aattttttca gcagcgttgt ttttccggtg ccactccatg cagcaaaggc
      541 gagtaacggt atcatcgttt ttcctgccat cgggcaagct cctctggcgt attcacgttt
      601 ataaatgcct ctttacgatc gctgaaatca accgcatgac cgccagccag acgcataaat
      661 gccataaccc ggcgttctcc tgcttgcaga tattccagta ataaaggctc aattgcgcgg
      721 tttaccagag caatagtcgg gtgatcgcgt tcaccgtcgt ggacccacac gacaggcgca
      781 tctttgcgct gatgagtaag ccgggcggct aaatcatggg gaatgtaagg cgtatcgcac
      841 gggcaaaaca aaaaccactc acccgcttcc tgctgcatta ctgaaagcat tcctgccaga
      901 gggccagggt aatccgccag tgaatcttca atcactttca gaccgcttac ctgatagatt
      961 tcctgatgac gattagcatt aaccacgacg tgagaaagct gcgtcataag cgcgtcagcg
     1021 acatgttgcc ataatggttt gccgtttaat tcaagcaatc ctttatctac gccgcccatt
     1081 cgtctggctt taccgcccgc cagcacaacg cctgttatcg tcgtcatcag attcaccgat
     1141 atcgcctctt ttattgtggg attgaccctg ctaacgtgtc tgtctcaaga gtaaggagca
     1201 ttactatgaa atgtaaacgt ctgaatgaag ttattgaact cctccagcca gcctggcaaa
     1261 aagagccaga ccttaacctg ctgcaatttt tgcagaaact ggcgaaagag tcaggttttg
     1321 acggcgaact ggcggatttg acggatgaca ttctgatcta tcacctgaaa atgcgtgatt
     1381 ccgcgaaaga tgcggtgatc ccgggtttgc agaaagatta tgaagaagat ttcaaaacgg
     1441 cgctattacg cgcacgtggc gtaattaaag agtaaaagct tgtaagcggc gcaaccaaaa
     1501 tcatcgtgaa atgatatcct tcatcattcg taatgttttc cggatgatgg gatgaacaac
     1561 agcgctttta ctttccagac actacacccg gataccatca tggacgctct gtttgagcat
     1621 gggatccggg tggattccgg tcttaccccg cttaacagct atgaaaaccg tgtctatcaa
     1681 tttcaggacg aagatcgtcg acgttttgtc gtcaaatttt atcgccctga acgttggaca
     1741 gccgatcaaa tcctcgaaga acatcaattt gcgttgcagc tggttaacga tgaagttccg
     1801 gtcgcagcac ctgtggcctt taatggtcag actttattga accatcaggg attttatttc
     1861 gctgtttttc caagcgtcgg tggtcgccag ttcgaagctg ataatatcga tcagatggaa
     1921 gcggttgggc gttatttagg gcgtatgcac cagacggggc gcaaacagct ttttatccat
     1981 cgcccgacca tcggtttgaa tgaatatctc attgagccac gcaagctgtt tgaggacgct
     2041 acactgatac cttccgggtt gaaagcggca ttcctgaaag cgacagatga gctgattgcc
     2101 gccgttacag cacactggcg ggaagatttc accgttctgc ggctacatgg agactgccac
     2161 gccgggaata ttctctggcg cgatggccca atgtttgttg atctggatga tgcacgtaat
     2221 ggtccagccg ttcaggattt gtggatgttg ctcaatggcg ataaagccga gcagcggatg
     2281 caactggaaa ctattattga ggcttatgaa gaatttagcg agttcgacac cgctgaaatc
     2341 ggactgattg aacctttacg cgccatgcgt ttggtttatt atcttgcctg gctaatgcgg
     2401 cgttgggctg atcccgcgtt cccgaaaaat ttcccgtggt taaccgggga agattactgg
     2461 ctacgacaga cggcgacttt tatagaacag gcaaaagttc tacaagaacc ccctttgcaa
     2521 ttaacaccta tgtattaatc ggagagagta gatcatgaaa aagatttggc tggcgctggc
     2581 tggtttagtt ttagcgttta gcgcatcggc ggcgcagtat gaagatggta aacagtacac
     2641 taccctggaa aaaccagttg ctggcgcgcc gcaagtgctg gagtttttct ctttcttctg
     2701 cccgcactgc tatcagtttg aagaagttct gcatatttct gataacgtga agaaaaaact
     2761 gccggaaggc gtgaagatga ctaaatacca cgtcaacttc atggggggtg acctgggcaa
     2821 agagctgact caggcatggg ctgtggcgat ggcgctgggc gtggaagaca aagtcacagt
     2881 tccgctgttt gaaggcgtac aaaaaaccca gaccattcgt tcagcatctg atatccgcga
     2941 tgtatttatc aacgcaggta ttaaaggtga agagtacgac gcggcgtgga acagcttcgt
     3001 ggtgaaatct ctggtcgctc agcaggaaaa agccgcagct gacgtgcaat tgcgtggtgt
     3061 tccggcgatg tttgttaacg gtaaatatca gctgaatccg cagggtatgg ataccagtaa
     3121 tatggatgtt tttgttcagc agtatgctga tactgtgaaa tatctgtccg agaaaaaata
     3181 attcaatgta aataacataa agcccgtgaa
//
LOCUS       DACADA010000280         3192 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.287, whole genome shotgun sequence.
ACCESSION   DACADA010000280 DACADA010000000
VERSION     DACADA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.287"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            65..556
                     /locus_tag="ISB17_004700"
     CDS             65..556
                     /locus_tag="ISB17_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1982537.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            559..1065
                     /locus_tag="ISB17_004701"
     CDS             559..1065
                     /locus_tag="ISB17_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HipA"
                     /protein_id="HAP1982538.1"
                     /translation="MALIGVYADWEGLDGPARIGYLHSRRTRAREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERVSRAI
                     EEDPDNKE"
     gene            1065..1826
                     /locus_tag="ISB17_004702"
     CDS             1065..1826
                     /locus_tag="ISB17_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HAP1982539.1"
                     /translation="MGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEY
                     DVGGWEMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLT
                     RHQDGEDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVTNTDDHLRNHGFI
                     LIPGKGWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDII
                     ANFRGIVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(1969..3177)
                     /locus_tag="ISB17_004703"
     CDS             complement(1969..3177)
                     /locus_tag="ISB17_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982540.1"
                     /translation="MDRTALGIAAPTIMETTGITKEQYSWIVSAFQLAYTLGQPIMGF
                     FIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIPAGVKTAST
                     WFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLALLAALFWFF
                     CYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIGIARFLADP
                     AWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFHDRGVSLIN
                     SRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLGGDLFKKDE
                     VATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIALWTLIKVKD
                     EEPQVQLATS"
BASE COUNT          916 a          654 c          773 g          849 t
ORIGIN      
        1 gttcaatcca ataaatgcta atattatggc tatcatagcc ataaaaagat aaaatattat
       61 cactatgggt aaaaaaacag caccgttact cccttatacg aatcagatgc ttagtgaatt
      121 tggtgagcgg ctgaaacttg ctcgtttacg acgcaggctt accgcaaagc aagttgctga
      181 acgtgctggg atgtcgccaa taacattgcg ctctctggag tcaggaagtg caggggtcac
      241 gataggggca tatttgtctg tgatgcaggt tttgggcctg gaaacggatt taaataagct
      301 tgcagctgaa gacgacttag ggcgtcagct ccaggattcc cgtttgatta aaaaaagagg
      361 gctgtctcgt gtatcaagta agaatgaaga aggttctcag acagcctcat tgcagatgag
      421 gcacgagaaa gaaggtcatc tcaccacgga tttaacagca ctggcagaag gctcgacgcc
      481 atctattaca ggagaaacat caacctctct tgctgcacta ttaaaaccga taaaaaaacc
      541 gttaaaagga aattaattat ggcattaatc ggagtgtatg cggattggga aggcctggat
      601 ggtcctgcgc gtattggtta tttacacagt cgtcgcactc gggcgcgtga gatatttgag
      661 tttgagtacg ataaaaaggc gcttgcagat ccttcactta atttcattca gttagatcct
      721 gaaataatgt tgtatgaggg agcacagtac ccaattcctc caaaggataa atttggtgca
      781 tttagtgatt cctgcccgga tcgttggggg cgtatgctaa tgaagcgtcg ttttgagagg
      841 gatattcgag acgggttatg tgataaagac tcccaccttt atgagtctga ttatctacta
      901 ggtgttcatg atctttatcg ggttggggct cttcgataca aacgtgaaga cgctggagag
      961 tttcttgata atcgcattga tgttgcagcc ccaccattta ccgaaatagc gagtctggaa
     1021 agggttagcc gggcaattga agaagatcct gataataaag agtaatgggg caaaaatggt
     1081 taagaatgct aatcgctccg gggggatcat tagggggggc acgtccgaaa gccagtgttg
     1141 ttgatgaagc tgggcatctc tacatcgcta aatttcccag tgtaaaagat gagtatgatg
     1201 ttggtggatg ggaaatggtc gttaatgcac ttgctgttgg ctgtggactg aatgttgcac
     1261 ctgctcaggc gcataaattt gccagcaatt accattgttt tatggttcgt cgttttgacc
     1321 gcactaatgc gggaaggcgt ctgcattttg cttcggctat gacactaacc aggcatcagg
     1381 atggtgaaga tgcctctaca ggtgtgagtt atctggaatt agcagatgtt cttatcagac
     1441 atggcgcgca gactaatact gaccttaagg aactctggtc caggattgtt tttaatattc
     1501 ttgttactaa taccgatgat catttacgca atcatggatt tatcttgata ccaggaaaag
     1561 gttggcggct ttctgaggca tatgatttga atcctgtggt caggtctgat ggtctaaaac
     1621 tcaatattac ggaaaatgac aatgcacttg atttggaatt agctagagaa gttgcagagt
     1681 attttcgact tagtttgacg gaagctgatg acattattgc taattttaga gggattgtca
     1741 gtcagtggcg aatcattgcc gagcgtcttc gattacctgg tcgtgagcaa gagttaatgg
     1801 cagaggcgtt caggggggca atttagaata gttatttcga tatattcgct aaatcataaa
     1861 tttgcgaagc aatgccagta gatactgaac gttttggaag agacgtttaa tttatgagaa
     1921 aaatagaaac gctacaaagc taaattatag cgtttctatg taaaataatt agcttgtcgc
     1981 taactgtact tgtggttctt cgtctttaac tttgataagc gtccacagcg caatggcgcc
     2041 aataatgtca aagaaagcca gggcaataaa gaacggcgcg aagccgataa tgtgaacgaa
     2101 tgccccgata aacaggttga aaatcaactg accgctccat gcacaggctc ctgccatacc
     2161 cacagcggta gctacttcgt cttttttgaa taggtcaccg cccagagttg ctgcaacagt
     2221 agaaagacat tgatgcgaga atgcgccaat gctaatcagt aatacggcga tgtagggatt
     2281 ttcgacaata ctcaccagac caatcgtcat cataatgacg gctgcaatag tgaaggtaat
     2341 tcttcgtgag ttaattaaac tcacgccgcg atcgtggaag aacttcgcga caaaaccact
     2401 ggctaaacag ccgagatcgc ccagcaggaa aggtagccag acgaacatgg caatttcttt
     2461 caggctaaaa tgcagcgttt cgacgaagaa aatcggtacc cagaagttaa tggttcccca
     2521 tgccgggtca gcgagaaaac gcgcgatgcc aatcccccag aaattacgtt ggctgaggat
     2581 atgcttgatt gacgtttttt ctttcttatc tgttttaaga tgctgttctt gcccagactc
     2641 aatatagtgg cgctcttcat cagaaaggcg tttagcatct tttggatctt tataacaaaa
     2701 gaaccaaaat aaagccgcga gtaaagcaag gctacctgag acaataaacg caaattgcca
     2761 gctatgaaac ataatgcacc aggcaatcaa cggtggagca agcatcgcgc cgagtgaggt
     2821 gcccatattg aaaacacctg tcgccacgcc acgctctttt gccgggaacc atgttgatgc
     2881 ggtttttaca cccgcaggaa ttgccgatgc ttcgctgaaa cccatcaggg cgcgcataaa
     2941 tgccagacct gaccatgttc ctgtcaaggc atgccccatt gtcgccaggc cccagattgc
     3001 ggcacatatc gcaaaactta acttcagacc cacggtatca ataaagaagc ccattatcgg
     3061 ttgccccaat gtataggcca actggaatgc actgacaatc catgaatatt gttctttggt
     3121 tattccggtt gtctccataa tcgttggagc cgcaatacct aacgccgttc tgtccagata
     3181 attgattaca gt
//
LOCUS       DACADA010000281         3171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.288, whole genome shotgun sequence.
ACCESSION   DACADA010000281 DACADA010000000
VERSION     DACADA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.288"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..211
                     /locus_tag="ISB17_004704"
     CDS             <1..211
                     /locus_tag="ISB17_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP1982541.1"
                     /translation="MTDGWPLYESRLKRKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     LLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(371..502)
                     /locus_tag="ISB17_004705"
                     /pseudo
     CDS             complement(371..502)
                     /locus_tag="ISB17_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            784..2142
                     /locus_tag="ISB17_004706"
     CDS             784..2142
                     /locus_tag="ISB17_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAP1982542.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2381..>3171)
                     /locus_tag="ISB17_004707"
     CDS             complement(2381..>3171)
                     /locus_tag="ISB17_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP1982543.1"
                     /translation="QSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHGYVMTDGK
                     LHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHL
                     ACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQ
                     LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYS
                     LIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          791 a          827 c          839 g          714 t
ORIGIN      
        1 gatgacggat ggctggccgc tgtatgaatc acgcctgaag agaaagctgc acgttatcag
       61 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac atctggcaag
      121 gctgggacgg aagttactgt cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg
      181 gcattatctg aacataaaac actatcagta agttggagtc attacctcct cccgaactca
      241 aagctatcat acagataatt actggttaac cctatatagg gcattttaaa gtgtgatctt
      301 atccagcaat aatggacacg cggctaagtg aaccgccccg ggtttcctgg agagtgtttt
      361 atctgtgaac tcaggctgcc agatcatcgt ttccgatgga agcataataa gctttttctg
      421 cttctgccgg tgggatatgg ccctgccttt ccagcaatcg tcgattgtta taccagtcca
      481 cccacgtgag tgtggtcagc tcatcatact gacacgtgca ttacccctac cgcgataatg
      541 caaccgtaca atatagggcg tttcctaaac gcagccctca tcactccaat ccaacaagac
      601 acagcatttt tgatcacgaa gaaagacaaa taatttccgt aaatgtttcc gttgttacaa
      661 ataacaacag tgttttacat ttctttggcc tcaggcccgc gcatgttgcc attcgatgtc
      721 attttcctga ctcgtttctt ctttatggtg tagtgtgatc ttcactttat ggatgctaac
      781 aatatgaaaa gaaaaattat tcccgtgcta atcggttgta cgctctcttt ctctgccctg
      841 gcggcacaac ctacggctga gcgctatgtc gtcagttttc cagaaggcac gcacgtgaac
      901 tacgccggcg catttgccag tgcattcccg aacggccttc cggttgggat aggttctggc
      961 ctgctgttca ccggtaaaca gggcgatgcc ctgacattcg caaccattac cgatcgcggt
     1021 ccgaacgcgg attcaccgaa agaggggaaa aacgaaacta aaattttcgt tacgccagat
     1081 ttcgcgccgc tgctgatgac gattcgtgta caaaacggca aagcggaggc catcgatccc
     1141 cgtcctctgc atgacgataa aggcgcgatc aacggtctgc cgctggcaag cgacgtgatt
     1201 ggctccacca atgaagtggc cttcagcgac acgttacata gactgaaggg ggataatcgc
     1261 gggctggata cggaagggat cactccggat ggtaaaggag gttactggct gtgcgatgag
     1321 tatggcccgt tcctgattaa catcgacagt aaagggaaaa tcctggcaat ccacggtccg
     1381 caggcggcgg aaggggaaaa agcgattgcg ggcggtttgc caaatatcct caaatggcgt
     1441 caggcgaacc gcggctttga aggcctcacc cgtatgccgg acgggcgtat tattgtcgcc
     1501 gtgcaaagta cgctggatat cgacgctaag agcaagaaaa aggcgctgtt tacgcgtctg
     1561 gtgagcttcg atccggcaag cggaaaaacc gcgatgtacg gttatcctat cgacagcgcg
     1621 gcctacagca aaaacagcga cgctaaaatc ggagacatcg tggcgctcga taaccagcac
     1681 atcctgctga tcgaacaggg aagagataaa aacaacagaa tgcgtaacct gatttacgag
     1741 gtcgatctca ataaggcgag cgacctgtca ggcttcgaca agccgggtga gtatccggag
     1801 tttgatgatg agaaaaccct gtcacagcgc ggcattaccc ttgcgcagaa aacgcaggtt
     1861 gttgatctgc gctccttggg ctggcagcag gagaaggctg aagggttggc gctgatcgac
     1921 agcaaaacgc tggcggtggc gaacgataac gactttggcg tgaaggtggc gatgcaacat
     1981 ccggtcgagg gcaagacatt caaggattat cgggtgaacg cggaaggaaa gctgacgctt
     2041 gacgacaagc aggttgaaac gaccctcaga gtgaagccac tggagaaacc ggagtctgac
     2101 agtgaactgt ggattgtgac gctgccggaa gcgttgaaat aagtttataa ggtacggaca
     2161 gcagtatcgc ttagtttttc aaattcatgt atgtgaacaa tattggggtt ccggcgtaga
     2221 cccccaatga aagcgtcacc acgtgaaaac atgcctgcac ggtttacacg tcatctcatg
     2281 gtggcagccg gttaactttc ctgctacacc agtggaaaaa ggccataagc agtccgaaca
     2341 aaagtaagcg tacagcgagg gccgtattga cggggatgtg ttattcagct ggcagtgcta
     2401 tgcgccacgg aagcagttcg ctgacccggt tgaccggcca gtctgctatg acgccaggca
     2461 catggcgaag gtagctttct ggatccacgt cattcagttt gcacgtcccg atcaggctgt
     2521 acagtagcgc tccccgctca ccaccatggc cagagccgaa gaacagggtc ttcatctttt
     2581 cacgcaacca gctttccagg gatttcaaca gcggtttcgt ttttcgctga cgttcagcaa
     2641 gccgctgctc tgccggcatt ccccttatat ccgcctctat ggcgtacaac tgaccgatct
     2701 gctctagggc ttcttccgtc agtgctgacg ggatgcggac gtgcacatcg tggatctttc
     2761 ggcgggcatg agcccagcag gcagcttccg ttatcccacc attgcgatac agctcgttga
     2821 acccggcgta cgcatccgct tgcagcacac cgctgaagca agcaagatga gtctgcggat
     2881 ggatgccttt tctgtccggg ctgtaagcga accacactgc aggtgccaac gctgacccgg
     2941 cattgcggtc atcacgaaca tacgcccaca accgcccggt cttcgtcttc ttattacccg
     3001 gcagcagtac ctggaccggg gtatcatcgg catggagttt gccgtcagtc atgacatagc
     3061 catgaagcgc ctcttccagc ggagacagca gccggcagca tgcatccacc cagcccgaca
     3121 gcagtgaacg gctcagctcc acaccttgcc ggccgtatat ttctgactgg c
//
LOCUS       DACADA010000282         3143 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.289, whole genome shotgun sequence.
ACCESSION   DACADA010000282 DACADA010000000
VERSION     DACADA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.289"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            45..281
                     /locus_tag="ISB17_004708"
     CDS             45..281
                     /locus_tag="ISB17_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP1982544.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(313..939)
                     /gene="tetR(A)"
                     /locus_tag="ISB17_004709"
     CDS             complement(313..939)
                     /gene="tetR(A)"
                     /locus_tag="ISB17_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP1982545.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1045..2244
                     /gene="tet(A)"
                     /locus_tag="ISB17_004710"
     CDS             1045..2244
                     /gene="tet(A)"
                     /locus_tag="ISB17_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP1982546.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2276..3136)
                     /locus_tag="ISB17_004711"
     CDS             complement(2276..3136)
                     /locus_tag="ISB17_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP1982547.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          598 a          987 c          959 g          599 t
ORIGIN      
        1 gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc
       61 ttagcgtagg attccgcccc ttcccgcaaa cgaccccgac ccgcgccacg gcccacgagc
      121 ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt
      181 ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt
      241 ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg
      301 cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct
      361 ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc
      421 ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac
      481 ggtgccgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag
      541 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc
      601 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg
      661 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc
      721 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc
      781 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa
      841 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag
      901 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat
      961 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag
     1021 cggaggctgg tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc
     1081 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg
     1141 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat
     1201 gcgttgatgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg
     1261 cggccggtct tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg
     1321 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact
     1381 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac
     1441 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg
     1501 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc
     1561 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta
     1621 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc
     1681 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt
     1741 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt
     1801 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc
     1861 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc
     1921 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg
     1981 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt
     2041 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc
     2101 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt
     2161 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc
     2221 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag
     2281 gcgacttccg gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga
     2341 tactcccgat cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga
     2401 acaaccatcc tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg
     2461 taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca
     2521 ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa
     2581 acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg
     2641 tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa
     2701 ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca
     2761 ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg
     2821 ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg
     2881 tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg
     2941 aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc
     3001 ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg accgtcatcg
     3061 gtgagaagtt cggcaggtat tgggtggtga caatgtaggt gctgccccaa atggcaggtg
     3121 ctatcgctgt gaacaataaa tcg
//
LOCUS       DACADA010000283         3137 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.290, whole genome shotgun sequence.
ACCESSION   DACADA010000283 DACADA010000000
VERSION     DACADA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.290"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(239..1252)
                     /locus_tag="ISB17_004712"
     CDS             complement(239..1252)
                     /locus_tag="ISB17_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HAP1982548.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVMGFGHHYLAQPTVVLHRSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDLPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(1264..2580)
                     /gene="fucP"
                     /locus_tag="ISB17_004713"
     CDS             complement(1264..2580)
                     /gene="fucP"
                     /locus_tag="ISB17_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAP1982549.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            complement(2608..>3137)
                     /locus_tag="ISB17_004714"
     CDS             complement(2608..>3137)
                     /locus_tag="ISB17_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1982550.1"
                     /translation="KLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGTQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          952 a          720 c          671 g          794 t
ORIGIN      
        1 tttttgttga ccactacaaa taaataaaag agttgcagtg cttgctcggt aagaaaacaa
       61 tggaagttga gatcctaaaa gaagctgtgg agtacaccac tctacagtag ttgcgcaaat
      121 ttggagtgga cgataatgtg atcgtctata agggcaacgc tatcatagtc ttgtcctggc
      181 gggtaaaaaa acgcgcttac cttaacgata agcgcgccgc tgttcaggcc ttaagtggtt
      241 attcaattcc tgtggtgact gtaaaagtgc gtgtttgctg cggtgcaacc tgaatcagcg
      301 tgccattacg ttgcgcggca agatacccct caggccgaca ggttgcaggt aatgcaaagg
      361 cggctacctg ttgctcgccg ttataaagga tccagcgtgt cacataattt agttcggcac
      421 tggagaaacg agtaacaaac gtagtgccat cgggagcgat catgcgaaac tctggcagat
      481 ctgtgtaagc gtccagtttg tctgcaaaga agactatttc tggatcataa aattccggtt
      541 gattcagcgt cgacagagag gcttctccct gcataatccg ttgattaaac gccagccact
      601 gagcggtggg attaacatgc gaaggtactg attcacgcaa ccttaatatt tcgtccggta
      661 tattctggct gaacgtagca tttggtatat atgcataatt catgtggcac atatattgta
      721 gtggcatatc tacagaagcc agattggtta cggccatctt aatatcgaac aatgtagagg
      781 atctgtgaag gaccactgtt ggctgagcca gataatgatg cccgaacccc attacatact
      841 cgtaacgccc ggtaaggcgt aacatatctc cctctaattc catccatgct tcatccatcg
      901 cggcacaggc catttcaccg tgtagcagat gagtatcttc cgcagatggg cagccattag
      961 ccagcaaacc tgaatgaaag gcaaaacagc cataggtttc tatcacctct gtcgccggtt
     1021 taggctggcg aaacatattg cacatggtga gactgtgtcc atcaaattgc gcatcccaaa
     1081 tcatctgccc catccaggga agaataatta aatgtccacg actgtttgca attttaagcc
     1141 cctcgacacc actgtcatag cgaaaagacg tgacagtaaa atcactattt tccagcaaga
     1201 tacgaggttt ttcgccaaaa agcgcccgcc acaaataaat acgcgtactc ataacgattc
     1261 tcctcaggac tctgtgactt cagccagtgc attacgtact ttgctttcac gccagaagta
     1321 gacaccgaca tagacaaaac agagcataga aaccaggaat gaaagctgta gtgagtggaa
     1381 catatctgca atatacccct gaattgccgg aaccaccgct gcgccaacaa tagccataac
     1441 aatgactgct cctgccattt ctgtatgttg attatcaact gtatccagtg ttcctgcata
     1501 gatcgtcgcc cagcaagggc caaacaaaac acttaccagg acggcgacat agaccgcgct
     1561 gaaacttgga gccagtgcaa catatgccag aaacagcgcc cctataacgg aatagagaat
     1621 taggactttt tccggattaa aacgtgtcat aaggatgttg gctataaact tgccaataaa
     1681 gaagcaggca aagctgtaga ccatgaagtt tgaagcatca cgttcgttga tatcgcccaa
     1741 ctccagtgcc agacggatgg taaatgacca tactgcgacc tgcataccca cataaaggaa
     1801 ttgagccaca ataccacgac gaaagcgcga atttttagcc agatagcgca gcgtatccat
     1861 tgctgacggg cgtttatggt gacttgtctg tgccacttta caggttggga agcgggttaa
     1921 aaggaacaac accatgacca caaccagaat cataatcata tacttatacg gttcaagggt
     1981 gttctctaac atcagcacct taaagttgtg aatttgctcg gcgttcattc cggacatctg
     2041 cttctcaagg ctttccccct cggagaaaac cagatatttg cccaataaaa taccagacgc
     2101 agcaccaatc ggataaaagg tctggctgat attgagccgc aatgtggcat aggcttttgg
     2161 accgatcatt gaactgtatg tgttcgctgc agtttcaagg aaactcaggc caatcgcaat
     2221 cgcaaaaata gctgcaagga acatagtgta ggttgccata tgcgaggcag ggaaaaaaag
     2281 tgtacaacca ccaatataca gcgtcaggcc aattaaaatt gccaccttat aactggtctt
     2341 tttaatcaca agggatgctg gtattgcaat taaaaaataa cctccataaa atgcgctctg
     2401 caccaatgct gaagcaaagt tacttagcga aaatacactt ttgaattgag tgattaatat
     2461 gtcatttaat gcagctgcgc atccccatag cgggaataaa cacgataaca aaataaactg
     2521 gaacaaggga gtcttattca gatacccatc aggcatctga atgatgtttt tatcgttcat
     2581 agtgctacct ttaactgtgc aggatgatta ttcgtttaag gttaaaaatt cattaaattg
     2641 ttcaatactc ggataagatg attgcgtacc tttccctgtg acgctgaaag cggcaaagag
     2701 agcggctttt ttcaaagcgg cttcaacatc accgctttga acataataat gggaaaagca
     2761 accaataaat gcgtcaccag cgccactagt atcaacagca tttactttga atgcaggaac
     2821 atggacttcc tgatcgcggg tcatccataa tgcgcctttt tcgctcatgg taacaataat
     2881 attgttcagc cctttatcaa ctaacgaacg tgcggccaaa cgaatatgat cataagtatc
     2941 aaccgacata ccggttaata tttccagttc tgtttcattc gggataaaga aatcacattt
     3001 gcaggcataa gacatatcta actcacgcaa tgccggagcc ggatttaata acacttcaat
     3061 accatttttc ttaccaaact caatcgcgtg gtaaactgtt tccagttgaa cttccagttg
     3121 taaaacgatc aatttgc
//
LOCUS       DACADA010000284         3129 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.291, whole genome shotgun sequence.
ACCESSION   DACADA010000284 DACADA010000000
VERSION     DACADA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.291"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            629..859
                     /locus_tag="ISB17_004715"
     CDS             629..859
                     /locus_tag="ISB17_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982551.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            911..2272
                     /locus_tag="ISB17_004716"
     CDS             911..2272
                     /locus_tag="ISB17_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAP1982552.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            2320..2883
                     /locus_tag="ISB17_004717"
     CDS             2320..2883
                     /locus_tag="ISB17_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1982553.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2968..>3129)
                     /locus_tag="ISB17_004718"
     CDS             complement(2968..>3129)
                     /locus_tag="ISB17_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HAP1982554.1"
                     /translation="RKKKNSSSSSSSSSSSSSSSSSSRNNNISNRQMTGMKKQKPPSE
                     RPRNERGGA"
BASE COUNT          718 a          871 c          910 g          630 t
ORIGIN      
        1 cccttgtcgc gccgtggcct gcgctcccgg catcccggca aaaggccgcc gggatgtaag
       61 gggcaacgcc gtttcgcgca cgcgctctcc gtcgttgcgg cgggtccctg ctcattgcct
      121 gccggtgcca cacacccgta tgagtccctg tcgcctgggg ctgatacagc cacaacctgc
      181 cgcactgact gtccagggta aatgaacagc ccacaaaatt ttttacccaa aacccggtaa
      241 aaaattttgc gttctgccca ggacaggtgc gtcaggccgt ggcagtgatg ccccttgcgg
      301 cgaaggttct caaacgggtg aagttcatac cacagggtat acagcagcag gttcccgcga
      361 aacgtcaaaa ccgcggctcc cgaaactcaa cgggtgatgt tctttaaaaa tcaggattta
      421 tttcaggctc cggcaatacg catttatgtg ttctgtcagt catccggctg gcaggtaatg
      481 agaacgcagg aacagaagga ggaaaacacc ggagaacgtc agacgcagat atgaaaaaag
      541 caggtcaggg acctgcttaa tccacaccgc agagactgca cgaacagtct ttacggtttc
      601 attaattaac gtcataagag gctaaattat gacagtaagt attgtttcac cttccgcagc
      661 ggctgtaaag ccccgccgtc atccccgttt cagacgcgaa gacatccccg cgccggaaat
      721 tgaccccgtg ctgaaagcct tcgggcgaca cattgcccgg agttttcacc gggggcgtgg
      781 tgtacacatc cccgccatga aaaacacggc cttcgggcag gtgctccgca cactcgaact
      841 gaaacgcgct tttaactgat gaggtgagcc gccccgcgag gggcggcaac agacggaagg
      901 agaacatttt atgacattag cagcaatcac catgaccgcc ccggaagccg ccagccctgt
      961 gcagatgtac cgcgcgacct actcaccgga tgacaacaaa ctgcgcctgt atgccgtgtc
     1021 acgtcttgac ccggagacgt ataaaaaagt gcatgatgcc ggtttccgct gggcaccaaa
     1081 acaggcgctg tttgtcgcgc cagcctggac accgggccgg gaagacgtgc tcctctcact
     1141 tgccggagag attgaggatg aagacagcac gctcgctgaa cgtcaggaag cacgggcgga
     1201 gcggtttacc ggatacagcg gaaagcgggc cagtgaatcc gcacaggcac ttgatgaagt
     1261 ggaaagactg gccgcgatga tcccgcccgg tcagcccatt cttgtggggc atcacagcga
     1321 acgccgcgcc cgtcgtgatg cgcagcgtat tgaaaacggc atgaaacgag ccgtgatgct
     1381 ctttgaacgt gcggaatact gggaagagcg ggcgcggtcg gcactgcttc acgcgaagta
     1441 taaagaacgt ccggacgttc gctggcgtcg tatcaaaaaa atcgaagctg atttgcgcaa
     1501 ggctgaaaag accatcgcgc agtcgcagaa atatctgacg atgtggcggg ctgaatcgct
     1561 ggatctgaat atggcaaaac tcatcagcag tcatgaccat atcagcgcct gtttcccgct
     1621 ggatacgtat ccgcgcccgg cagaaaaaag ccagtatgaa ggaagtcgat cgttatggtc
     1681 ggccctggat gatgacatca tcaccacgga gcaggcccgc gaaattgcga tccgctgtca
     1741 tgaacggcag attcagcatc aacaacgctg ggttaaccac tatcagaacc gcctgatcta
     1801 tgagcgtgcc atgctggacg aaagcggcgg cgtggttacc cggacacagg attttgagcc
     1861 gggcggacag gttttcagcc ggggcgagtg gctgaccatc atccgtgtga acaaaagcaa
     1921 cggggcggtg agttcagtca caacgccaaa ttacagtttt ctcgggtaca gcggcacgat
     1981 gaaagtgacg cccgatcgca tcacggacta caaagcacca tcggcagaag aggctgccgt
     2041 cgccagtcag gccgcgaagc gtccgccggt agtcaactat ccgggggaag gtttccggga
     2101 aatgacaaag gcacagtggg ccgccctgcc ccgggactgt aaggccgtgc gcagtgtggc
     2161 agaagcagaa gaccacgggg cataccggta ccgccgcaca atggacaata atttccgcct
     2221 ggtgaatgtg tatatctccg acatgaaaat cacggaaatc ccacagaaat aaggtatatc
     2281 cccccgggaa aatccccggg ggatgacgga gataatgata tgcacagtca gttaagagaa
     2341 cgtatccggc tgatgcgcgc aaggctggat aacgccgcgc cggttgctga aatccgggct
     2401 gaatctcagc tttttgtgac tcctgccccg gtctgtgatc gcctggtgat gctggcggag
     2461 atcagcaacc gtgatcacat tctggaaccc tctgccggca ccggggccat actgcgggcg
     2521 attcgggata ccgcgccggg ggccatgtgt gatgcggtgg aaatcaacag cgggctggtc
     2581 cggtatctgc gggaaaattt taacggtgtc agggttcagt gtggcgactt catggaatgg
     2641 ctgccggtgc agtattacag ccgggttatc atgaatccgc cgttcagcca cgggcaggat
     2701 atccggcata tcctgcgggc cttttccctg ttgcgtccgg gtggcgtgct ggtcgccgtc
     2761 tgtctcaacg gaccacgcca gcaggagaag ctgttaccgt tttctgacgt ccgcgaggag
     2821 ctgccgcgcg gcacgtttgc ttataccgat gtcccgacga tgattattcg tctgcgtgcc
     2881 tgatgacaca gggccggcgg gaggataaat gcctgtctgc ccgcgccggg aaaaaacgcg
     2941 cgccggtggc gctgaaaggg gccgcgctca cgcgccgccc ctttcattac gcggacgttc
     3001 tgatgggggc ttctgtttct tcatgcctgt catttgtctg ttactgatgt tgttgttcct
     3061 gctactgcta ctgctactgc tactgctact gctactgcta ctgctactgc tactgttctt
     3121 ctttttcct
//
LOCUS       DACADA010000285         3108 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.292, whole genome shotgun sequence.
ACCESSION   DACADA010000285 DACADA010000000
VERSION     DACADA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.292"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..2931
                     /locus_tag="ISB17_004719"
     CDS             <1..2931
                     /locus_tag="ISB17_004719"
                     /inference="COORDINATES: protein motif:HMM:NF014452.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107086997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982555.1"
                     /translation="NKNKDLKFHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAY
                     KGAGVEIGKDTVVDWSVKGEANDNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAF
                     NAIYVASGRGTVKLGQADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTI
                     ITNTSDKTATLSLQNPSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNINTRNDITV
                     RNSQLRLQGHATSHAIFREGPRHCYVPGVLCDKDYVTDFARLESEANKKNNSAYKTNN
                     QVASFDQPDWETRHFRFKTLNLENSEFTTARNSVVEGDIVASNSTLKLGGDVPVFIDM
                     YDGINITGNGFGFRQDVREGRSADDGSSSYTGKITLQKGSTLDINNRFIGGIEAHDSK
                     VNVTSPDALLQNSGVFVNSTLSVRDGGHLTAQKGLYSNGRVQIGKKGTLSLSGTPENG
                     ADNTWMPVLTYMTEGYDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSS
                     VSPVLAAGLFSGYNAAYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEEN
                     GAFRTLTVKTLDATGSDFVLRTDLKDADKISITEKASGSDNTLNVSFMKNPSPGQSLN
                     IPLVSAPAGTSGDIFKAGTRVTGFSRVTPTLRVDTTGGSTKWILDGFRTEADKAAAAK
                     ANSFMNAGYKSFMTEVNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQ
                     VGFDKKHALDGVDLFTGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLI
                     GKYIHHDNDYTGNFAGLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSG
                     KTFRWKDGEMDLSMKNKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGE
                     TVLRDASGEKRIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANF
                     RYMF"
BASE COUNT          850 a          703 c          850 g          705 t
ORIGIN      
        1 aataaaaata aagatttgaa attccatggt ggcgggagca ttgagctcac cgataacctg
       61 aactcaggaa ccggaggatt gatttttgat gagggacaac attattcggt tattgggaaa
      121 gataaagcct ataaaggggc gggtgttgag atcggaaaag atacggttgt cgactggtcg
      181 gtaaaagggg aggcaaacga taacctgcac aaaacagggg ccgggacact gaatgtcaat
      241 gtggcccagg ggaataacct gaaaacaggt gacggtaccg tttttcttaa tgcagaaaag
      301 gctttcaatg ctatctatgt tgccagtggc cgtggaacgg tcaaactggg gcaggccgat
      361 gcgctggata aaaatagtga ttacagaggt atttatttta ctagtcgtgg ggggactctg
      421 gatttaaacg ggttcagcca gtcgttcaag aagatcgcgg caactgatgt tggtaccatt
      481 atcaccaata cttctgataa aacagcgacc ctttccctac aaaacccctc ccgctatgtc
      541 tatcacggta gtatcacggg aaatacgaat atcgaacaca ctgggacaca gaaaagtgct
      601 gacagcagtc tgattattga tggaaacatt aatacacgca atgacattac tgtgcggaat
      661 tcccagctca gacttcaggg gcatgccaca tcacatgcaa tattccgcga ggggcctcgg
      721 cactgctatg tccccggagt tctttgtgac aaagattatg ttactgattt tgccagactg
      781 gaaagtgagg caaataagaa aaataacagt gcctataaaa caaataatca ggtggcttct
      841 tttgaccaac ctgactggga aacccggcat ttccgattta agactctgaa tctggaaaac
      901 tcagaattca caactgcacg taactcagtt gttgagggtg atattgtcgc atcgaattca
      961 acgctgaaac tggggggcga cgttccggtg ttcattgata tgtatgatgg catcaatatc
     1021 accggtaatg gttttggctt ccgccaggac gttcgtgaag gacgctcagc agatgatggc
     1081 agtagcagct atacaggcaa aattacactg caaaaaggct ccacgctgga catcaacaac
     1141 cggttcattg gcggtattga agcccatgac agtaaggtaa acgtcacctc accggatgcc
     1201 ctgctgcaga acagtggtgt tttcgtgaat tccacccttt ctgtccgtga cggcggtcat
     1261 ctgacggcac aaaaagggct ctacagtaac ggccgggttc agattggaaa gaaaggtacg
     1321 ctttccctga gcggcacgcc ggaaaatggc gctgataata cctggatgcc cgtactgaca
     1381 tacatgacag aaggctatga tttaaccggt gataacgcta cgctgaacat cagccagcag
     1441 gcgcatgttt ccggggatgt tcatgcaacc agttcatcca gcattcgtat tggctccgaa
     1501 aaccctggct cagtttcctc ttctgtctcc cctgttctgg ctgccgggtt gttcagcgga
     1561 tataacgcgg cgtactacgg tgccatcacc ggcggtaagg ggaacgtcag tatgaataat
     1621 ggcctgtggc agctgaccgg agattccgac atcaacagtc tgacgacccg taacagccgg
     1681 gtacagtctg aagaaaacgg tgccttccgt accctgacgg ttaagacact tgatgccacg
     1741 ggcagtgatt ttgtcctgcg cactgatctg aaggacgctg ataaaatcag tattacggag
     1801 aaagccagcg gttcagacaa caccctgaat gtcagcttta tgaagaaccc gtctccggga
     1861 cagtccctga atatcccgct ggtcagtgca ccggccggaa catcagggga tatctttaag
     1921 gccggcaccc gggtgacagg cttcagtcgt gtgacgccga cgctgcgtgt tgacaccact
     1981 ggcggcagta cgaagtggat tctggatggt ttcaggacgg aagctgataa agcggctgca
     2041 gcgaaggcga acagtttcat gaatgccggc tacaaaagct ttatgacgga agtaaacaat
     2101 ctgaacaaac gtatggggga gctgcgtgac acgaacggtg atgccggtgc ctgggcccgt
     2161 attatgaacg gcgccggttc agccgatggc ggatacagtg ataactacac ccacgttcag
     2221 gtcggttttg acaaaaaaca tgcgctggac ggtgttgacc tgttcaccgg tgtcacgatg
     2281 acctataccg acagcagtgc agacagtgat gcgttcagcg ggaagacaaa atccgtgggg
     2341 ggaggtctgt atgcttcagc actgtttaac tccggtgcct acattgattt gattggtaaa
     2401 tatattcacc atgacaatga ctacacaggt aactttgccg gtctggggac gaagcactac
     2461 ggaacccact cctggtatgc cggagcggaa acgggttacc gttatcacct gacggaagac
     2521 acatttattg agcctcaggc cgaactggtt tacggcgcag tgtccgggaa aacattccgc
     2581 tggaaagacg gtgagatgga cctgagtatg aagaacaagg atttcagccc gttgattggc
     2641 agaacaggga ttgagctggg caaaaccttc aggggtaagg actggagtgt gacagcccgt
     2701 gccggaacca gctggcagtt tgacctgctg aataatggcg agacagttct tcgtgatgca
     2761 tccggagaga aacggatcaa aggggagaaa gacagcagga tgttgttcaa tgttggcatg
     2821 aacgcacaga taaaggacaa catgcgcttt ggtctggagt ttgagaaatc cgcctttggt
     2881 aaatacaacg tggataacgc aataaacgcg aatttccggt atatgttctg acaaggagag
     2941 gcccctgaag aggggcctgt agtgatgtta ctcccgggtt aatgcagcta gtaaaggtat
     3001 ggctctgact accctggaaa gtggtgccca tcgcatcact acaaattctg gcaattgcgt
     3061 acgtggtaac ggcaagttat ttaacctcaa acgtacgtgc ccgagcgg
//
LOCUS       DACADA010000286         3103 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.293, whole genome shotgun sequence.
ACCESSION   DACADA010000286 DACADA010000000
VERSION     DACADA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.293"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            48..1163
                     /locus_tag="ISB17_004720"
     CDS             48..1163
                     /locus_tag="ISB17_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982556.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADQEGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1756..2607
                     /gene="hchA"
                     /locus_tag="ISB17_004721"
     CDS             1756..2607
                     /gene="hchA"
                     /locus_tag="ISB17_004721"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAP1982557.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2715..>3103)
                     /locus_tag="ISB17_004722"
     CDS             complement(2715..>3103)
                     /locus_tag="ISB17_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP1982558.1"
                     /translation="YLSDEKEICFKVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKS
                     RIHITSFLDANGSLNIDIASPGTKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIA
                     ELHGGSATYHYLSKHNVFRITLPQRN"
BASE COUNT          905 a          709 c          654 g          835 t
ORIGIN      
        1 cacttattga tatattcatg aagattataa tcacaaggga atacataatg aaaagaaaag
       61 ttctggcaat gctggtcccg gcgttattag ttgctggcgc agcaaatgcg gctgaaattt
      121 ataataaaaa tggcaataaa gttgattttt acggcaagat ggttggcgaa cgcatctggt
      181 caaatactga tgataataac agcgaaaacg aagatacctc ctatgctcgt ttcggtgtta
      241 aaggtgaaac ccaaatcacc agcgaactga ctggtttcgg tcagtttgaa tacaacctcg
      301 acgccagcaa gcctgaaggt gaaaaccaag aaaaaacccg tttaaccttc gccggtttga
      361 aatataacga gttaggatca tttgactatg gccgtaacta cggtgttgct tatgatgccg
      421 cagcttatac cgatatgtta gttgaatggg gtggcgattc ctgggcttcc gctgacaact
      481 tcatgaacgg tcgtaccaac ggtgttgcaa cctaccgtaa ctctgatttc tttggcctgg
      541 ttgatggtct tgattttgcg attcagtacc aaggcaaaaa cagcaaccgt agcactaaga
      601 aacaaaatgg tgacggctat gcgttgtctg tagactataa catcaatggt tttggtatcg
      661 ttggtgcata tagcaaatct gatcgcacca acgatcaagt tgccgatgga aacggttcaa
      721 atgctgaact gtggtcattg gctgcaaagt atgacgcgaa caatgtctac gcagctgtaa
      781 tgtacggtga aactcgtaat atgactccgg gttctattga tacaggtgtt gccgatcaag
      841 aaggcaatac aataatgcgt gatcaactta tcaatgaaac ccagaacttt gaagcggttg
      901 ttcagtacca attcgacttt ggtctgcgtc cgtccttagg ttatgtgtac tctaaaggta
      961 aagatatcaa aggtgtacct ggacatagat atgttgacgc tgaccgtgtg aactacattg
     1021 aagttggtac ctggtactac ttcaataaga acatgaacgt ttatactgca tacaaattca
     1081 acatgctgga taaagatgac gccgccatta ctggcgcagc agctgatgac cagtttgcag
     1141 ttggtatcgt ctaccagttc taatcagcca acgcgccgag gcgtgttact gtaaaacata
     1201 acaccagcct gagttcgtcc ctcatcgaac tcaggctttt ttattggcat ttcatctcca
     1261 tctgcggcac acgcaattgc caaagcatat cgatacagca cattaccccg cccctcctgg
     1321 ctcaataaaa tctaccagcg tctcttccag ccaacaccca aatgcatttc tttatgaatt
     1381 aatccgcaac aaaaccgctt cccatataga aatatttgca aaaatagtgc atgaaaaaaa
     1441 ctttttccct accaatccgg gatataaata ttcatcagta ccacaacatt ccacagcact
     1501 aaatctctcc ccatcgcccc caaacctctg ataatggtct aaaatcattg aggccacttg
     1561 cgacgacgtt actcaataag gaaagtttgg cgtgaataat attcaacacc agtgcagcat
     1621 catcattaca tacttatgcc cgccaactac cgctttcgat atattgactc aatttttcag
     1681 cctggttaaa aatagcgacc agctcagtcg caaatatagt gactacccta actaagcaac
     1741 aataaggaat acactatgac tgttcaaaca agtaaaaacc cgcaggtcga tattgctgaa
     1801 gataatgcgt tcttcccttc agaatattcg cttagccaat atacgagtcc cgtctctgat
     1861 cttgatggcg tggactatcc aaaaccgtat cgcggtaaac ataaaattct ggtgatcgcc
     1921 gcggacgaac gttatttgcc gaccgataac ggaaaactgt tctcgaccgg taaccatccg
     1981 attgaaacgt tgctgccgtt gtatcatctc catgctgcag gtttcgaatt cgaagtggcg
     2041 accatttccg gtctgatgac caagtttgaa tactgggcta tgccgcacaa agatgaaaaa
     2101 gtaatgccat tctttgaaca gcataaatcg ttattccgta atccgaagaa actcgcggat
     2161 gttgtcgcca gcctcaacgc tgatagcgaa tatgcagcaa tctttgttcc tggtgggcat
     2221 ggcgcactta ttggtttacc agaaagccag gacgtggctg ccgctttaca gtgggcaatc
     2281 aaaaatgacc gttttgttat ctccctttgc cacggtccgg cagcttttct ggcacttcgc
     2341 cacagcgata acccactgaa tggttattct atttgcgcat tcccagacgc cgcagacaaa
     2401 caaacgccag atattggcta tatgccgggt catctcacct ggtacttcgg cgaagagttg
     2461 aagaaaatgg gcatgaacat cattaatgac gacatcaccg ggcgagtaca taaggaccgt
     2521 aaacttctca ccggcgacag tccttttgca gcgaatgcgt tgggtaaact ggcggcgcag
     2581 gaaatgctgg cagcttacgc gagttaatcc ctgttacatt gtaatgtaag cgcataatca
     2641 atcgcagcca cgttaatact cacgacgtgg ctgcgttttt tcacggttaa cttatggcgt
     2701 actgaagccc gatgttaatt tctttgcggt aacgtaattc ggaacacgtt atgcttactg
     2761 agatagtgat acgtagcact tcctccatgt aattcggcaa tcgctttgac taaagaaagg
     2821 ccaagtccct gacctacgga atgacgcgaa ttatctcccc gccaaaatct acggaagagt
     2881 ttttcaggct cattaatttt cgttccagga ctggcgatat caatattaag agaaccgttg
     2941 gcatcaagaa aactggttat atgaatacgc gatttttctg gtgaatatcg aatggcgtta
     3001 acaataagat tcgataacat tcgttgcagt aaaattttat ccgcaaagat ttgttgattg
     3061 cacttgacct taaagcaaat ctctttctcg tctgaaagat att
//
LOCUS       DACADA010000287         3093 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.294, whole genome shotgun sequence.
ACCESSION   DACADA010000287 DACADA010000000
VERSION     DACADA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.294"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..739)
                     /locus_tag="ISB17_004723"
     CDS             complement(<1..739)
                     /locus_tag="ISB17_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982559.1"
                     /translation="MISESVISAAVRAQQSEDGKVGNCGRIWHVAFFFDGVGRNIDRD
                     ATDCRLSNIARLFRAYPDEQKNTGDTCYNKFYFSGMGTAYHDDSVDNIHALMDIGLDN
                     FLEDMKSLPEDTISDAGESVIKGEKKWTDVLGNLLEDLNNPVEWVKGIGKMAAKSVGK
                     AGIESTPWLRDSEAMSAYFMTGEPVRLEAAKRLFKKFYTENMKGDVPIKKISVSLYGF
                     DLGATLARKFLDNFLKEVCLKNKEKKHQ"
     gene            complement(736..1263)
                     /locus_tag="ISB17_004724"
     CDS             complement(736..1263)
                     /locus_tag="ISB17_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HAP1982560.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAIPVAYFLLCVGIS
                     IWGAPTGGVILVIHSELDRPILGFSVNGMAGANAFAHGGGSTTCCGDIRGKEAEVVWT
                     LSTTRAQYNTGLREEVRRITLPLPERKHGQDFLHVHFLPGDKVFLGWSEGAGSPYEKR
                     KEPSYPSRKNQEAQP"
     gene            complement(1307..2128)
                     /locus_tag="ISB17_004725"
     CDS             complement(1307..2128)
                     /locus_tag="ISB17_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HAP1982561.1"
                     /translation="MSSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYTPRVLKQLAARPDMDWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTVANNSDETVEIITLDEPLWQKIADRGDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQVVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            complement(2131..3087)
                     /locus_tag="ISB17_004726"
     CDS             complement(2131..3087)
                     /locus_tag="ISB17_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HAP1982562.1"
                     /translation="MTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNL
                     GHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELNSL
                     SDKIQKLSDDAATANADPADMAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQL
                     AAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIRLIANKGAVSVQAQHDLME
                     LLAKKSIEIVSTEDEIKITAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYGRQ
                     PKASEKVPMPEFPILSAVDSSDFCLECLLNAIKNDDAVVEGV"
BASE COUNT          730 a          871 c          629 g          863 t
ORIGIN      
        1 attggtgttt tttttcttta tttttcaggc aaacttcctt aagaaaatta tccaaaaatt
       61 tacgtgccag tgtagctcca aggtcaaaac cgtataatga aacagaaatt tttttgatgg
      121 gaacatctcc cttcatattt tccgtataaa acttcttaaa caatcgcttt gcagcttcaa
      181 ggcgtacggg ttctcccgtc ataaaatagg cgctcatggc ttcgctatca cgcaaccaag
      241 gtgtggactc aattcctgct ttgcctaccg acttagccgc catcttgcca atccctttaa
      301 cccactctac cggattattg agatcctcaa ggagattacc aagcacatcc gtccatttct
      361 tttctccttt aataacagac tcccctgcat ccgatatagt atcctctggc agacttttca
      421 tatcctccag aaagttatct aaaccaatat ccataagagc gtgaatattg tcgacagaat
      481 cgtcatggta ggcagtgccc attccggaga aataaaattt attataacaa gtatcacctg
      541 tatttttttg ttcatcagga tatgcccgga acaggcgggc aatattactc aaacggcagt
      601 ctgtcgcatc ccggtcgata tttcgtccca ctccatcaaa aaagaaagcg acatgccaga
      661 tccggccaca atttcccact ttaccatctt ctgactgttg tgctcgtaca gctgcggaaa
      721 tcactgattc acttatcacg gctgcgcctc ctgatttttt cgactcggat aagaaggttc
      781 ttttcgtttc tcatagggcg aaccagctcc ttcactccaa cccaaaaaaa ccttatcacc
      841 aggcaagaaa tgtacatgca aaaaatcctg cccatgtttc ctttcaggca aaggcagagt
      901 gatacgtcga acttcttcac gcagtcccgt attatactga gcgcgagtcg tactcaatgt
      961 ccagacgacc tcagcttctt tccctctgat gtcaccgcag caagtcgttg aacctccacc
     1021 atgtgcaaac gcatttgccc cagccatgcc gttcacgctg aatcctaata ttggcctatc
     1081 cagttcacta tggatcacca atatgactcc tccagttgga gctccccaga ttgatattcc
     1141 tacacacagt aaaaaataag ccacggggat cgctattagc atccgccaca cccaacgccc
     1201 ccagcgggca taaccacgcg taagcttttg gtcaaatttt tccagtcggg taaatagccc
     1261 catcagattt tattcccttt tatcaattgg ttggcaaaga tgtatgtcac tggcgaccaa
     1321 ggtcagacag cgatatttca tttcgtttca ccttttccag catgacctga aattcggctg
     1381 actcaagata cgctttcttt ccattcagat agctcaacgc ataaattttc tgatccagtg
     1441 gctttgtcaa tccgaccacc tgagccttat tcaaggcctt ctccaccata tttcgctgaa
     1501 tacagggcgg gaagtgctca ctcgttttca tggtgcgcca ctctcttaaa agcgcagata
     1561 catctccccg atccgctatt ttctgccaga gcggttcatc aagcgttatg atttccactg
     1621 tctcatcgct gttatttgcc acagtaagcg gcatgttcag cctttgccac cgcgtatctt
     1681 ccagcatcca ccatgattct atctgagaaa acattaccgg atgccagtcc atatcaggcc
     1741 gggcagccag ttgtttcagc actcggggcg tgtagtacct cagcagaact tcacgaccat
     1801 ccggcgtttt tgccatacat cccacagcaa gccgagacgc cagtggctcc agctgcaatt
     1861 cggagaaagt aatgcctgcg acaggtagtt tcccgacaag caccatcgcc agatcctttt
     1921 cactttcaat acgaaccaac cagggaccgt aagctgctac ctttttaaga tcgggcagtt
     1981 gatacaggga aattcgtcgg tatttataat gctccatcat gatctgaagc atttcctgag
     2041 agagcgctcc accttcaaat aaaataaagg cgtttaacgt ttcgtttcgc ttaagccagt
     2101 ggtctgccag ttgttgttct gaagacatga ttacactcct tcaacaacag cgtcatcgtt
     2161 tttgatggca ttcagcaggc attcaagaca aaaatcggat gagtccacgg ctgaaaggat
     2221 gggaaattct ggcataggaa ccttctccga ggctttaggc tgacgcccgt aatgtaccgc
     2281 tttgacgtta taatcccccg gcgttcccgg ctcaatgccg ctaccctcta tccggatata
     2341 actcccacca ccattaatcg ttattttttt cttcgcggtg attttaatct catcttcggt
     2401 actgacgatc tctatcgatt ttttcgccag taactccatc agatcgtgct gcgcctgtac
     2461 cgatactgcg cctttattgg caatcagcct gatacccgcc ttgcgtacaa acacgctcag
     2521 cgcccgtccc acgccgataa acatattctt cactacgccg atatcggcgt tgtttcccgc
     2581 atttatctgg aggtttttca ccgcggccag ttgcaggtgc tcgccgctgg cgaccgccac
     2641 gcctttcggt gcgtgcatca aaatcaccga ggcggtcagg tcatttatcc ggctggttat
     2701 caatgctatc tgcgcggcca tatccgccgg atcggcgttc gccgtggccg catcgtcaga
     2761 cagtttctgg attttatcgc tcaggctgtt cagttcgctg ataatgtcgg ccatttcgcg
     2821 cacctgaccc tgcgccttgt cctgcgcgtc ggcgctgata aagatgcctt tctttgcccg
     2881 tactgcaccc cacagatcgg tacgcagttc aaaaccttcc ccgcgctgct gtttcccggc
     2941 atccaccaga tgaccgaggt tgagctggct cttgccgccg tactcggtac tgaccttgat
     3001 atgctctttg ccgcgctcat cgtcgaggcg gattttgttg ttcgccgggg tgcgtagcac
     3061 gttgcgcttg tagttctgaa tggtcacatg gtc
//
LOCUS       DACADA010000288         3065 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.295, whole genome shotgun sequence.
ACCESSION   DACADA010000288 DACADA010000000
VERSION     DACADA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.295"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            191..1042
                     /locus_tag="ISB17_004727"
     CDS             191..1042
                     /locus_tag="ISB17_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1982563.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1069..2058
                     /locus_tag="ISB17_004728"
     CDS             1069..2058
                     /locus_tag="ISB17_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1982564.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2089..2982)
                     /locus_tag="ISB17_004729"
     CDS             complement(2089..2982)
                     /locus_tag="ISB17_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982565.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          801 a          785 c          736 g          743 t
ORIGIN      
        1 tatatttttt atggacattc atttttattc atcggactgt aaattccggg gatatcgaac
       61 ttgcacgact aatctcaacc ctcaaacaga tttatgaatt ttgttcataa cccctgacaa
      121 atcctgccgt ctaatcgagc tcttcccttt acgttatgct tctcatgaac gataacggaa
      181 ggataacacc atgcaaaaag taaaactgaa caacggtatt gaaatgcccc tgttgggctt
      241 tggtgtattt cagatgaccg atgccgccga atgcgaaaga gcggtcattg acgctattaa
      301 cagcggttat cgactgattg acaccgccgc ctcttatcaa aatgaaatcc aggtcgggaa
      361 cgcactgaaa caaagcggta tcgcccgtaa cgaactcttt gtcaccacga aactctggtt
      421 gcaggatacc agttacgaag gggccaaagc ccagttcgaa cgctctctga atcgactgca
      481 acttgattac gttgacctgt acttgattca ccagccatat ggcgatgtcc acggagcctg
      541 gcgtgccatg gaagagctgc aacaggccgg taaaattcgc gctattggcg tcagtaactt
      601 ccatcctgac cgactggcag accttatcgc ttttaataac gtcgttccgg cggtaaacca
      661 ggttgaagtt aaccctttca atcagcagct acaggccgtt ccctggatgc aaagtcgtgg
      721 cattcagcca gaagcctggg caccgtttgc ggaagggaaa aatggtctgt tccagcatcc
      781 tgttctcacg gcaattggcg agaagtatgg caaaagcgtg ggccaggttg ttctgcgctg
      841 gatctaccaa cgaggcattg tttcactggc aaaatcagtg cgaaaagaac gcatggaaga
      901 gaacatcaac attctcgatt ttgaactcag ccctgaagat atgctgcaaa ttaccgccct
      961 ggataccgca accagcgcgt tcttctctca ccgcgatccc gcaatggtgg aatggctgac
     1021 tggccgcaaa cttgatgttt aagccgcgat aaaagaggta ttcattcaat gcaaaaacgt
     1081 tatctgggta aatccggact cgaagtctcc gcccttgggc tcggttgcat gggcttaagc
     1141 cacggctacg gcccggcgac cgatacccgt caggctatcg aacttattcg tgctgcggtt
     1201 gaacgtggtg tcaccttctt cgataccgcc gaagtgtatg gtccgtatct gaatgaagaa
     1261 gtggtcggcg aagccttaaa accgtttcgt gaccgcgtgg taattgccac taaattcggt
     1321 tttaccttcg gcgatgataa taagcagcag attttaaaca gccgtccgga acatatccgt
     1381 gaagccgttg aaggatcatt acgtcgtctg aaaacagatg ttattgacct gctgtatcaa
     1441 caccgtgttg atccagatgt tcctattgaa gatgttgcag gaacggtgaa agacctcatc
     1501 gcagaaggta aggtcaaaca tttcggcctg tctgaagcag gagcacaaac cattcgtcgt
     1561 gcacatgcgg tacaacctgt caccgccctg caaagcgaat attccatgtg gtggcgtgag
     1621 cctgaacagg aaattctgcc gttgctggag gaactgggaa ttggttttgt ccccttcagc
     1681 ccactaggta aaggtttcct gacaggggcg attaagccgg gaaccacttt cggcaaagat
     1741 gattaccgca gcaccgtacc gcgtttcgca gcacaagcca ttgaagccaa tgaaaaactg
     1801 gtcaccttgt taggtgaact ggctgcagat aaaggggtaa cgtctgcaca aatcgcactg
     1861 gcatggctat tagcacaaaa gccgtggatt gttcccatcc ctggcaccac taaactgaac
     1921 cggctggaag aaaacctggc cgctgctgac attgttcttt cgcagaaaga tacgcagcag
     1981 ataaccgagg cgctggaaac aattaaaatc gtcggtgaac gttactcgcc tgaacatcag
     2041 gcacgcgtag gccgctaata cccaaacggg tccaggataa aggacccgtt attcggtgta
     2101 gcgaagcgca tcgatcaaca gcgcaaaagc tggcggatgc tgcttgcggc tggggtagta
     2161 aagataatac ccaggaaaag ttggacacca ctcctgtaac acctgaataa gcttgcctga
     2221 ctttatatac tcctgaacca tgtcttcagg gacacaagcg atcccaaacc ctgataacgc
     2281 ggcatcaatt ctttctggca atggactaca cgtcagttgc ccatcaacct taacgcgaag
     2341 cggtttccct tctcgctcga actcccagtg gtaaagccca ccggcagttg gcaaacgcat
     2401 gttgatgcag cgatgatttt gtagctcatg aggcgtttca ggcgcagggt ttgcagcaaa
     2461 atacgccgga gctcccacca cagccatccg catatctggt ccaatcctga ctgcaaccat
     2521 atctttatca acattttcac ccagtcgtat cccggcatca aaacggcctt caacaatatc
     2581 gacaaaaccg ttatccacca ccagttcgac attgatttcg ggatactctc tgagaaaagg
     2641 ttttagcttc ggccagacca gacttcgcgc ggcatgttcc ccggcagata accgaatatt
     2701 cccggagggc gtgccgttca attgaataag tgattccagt tcctgttcaa gctcgtcaat
     2761 acgcggttca aggcaggcaa tgattctctc ccccgcttct gtgggagcca cactgcgggt
     2821 agtccgtgtc aggaggcgga tattgagcct ttcctccaga gccttcattg catggctaag
     2881 tgcagactga gagacgccga gtttgcccgc tgctttggta aaacttcgct ctcttgccac
     2941 cacaagaaag atttgcagtt cgttgaagtt ttctttgagc attggcgatt tccttgtaga
     3001 agcatcctct tcacagacac tcatgaaggg gggcatagat gctattattt taaccacatt
     3061 attag
//
LOCUS       DACADA010000289         3058 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.296, whole genome shotgun sequence.
ACCESSION   DACADA010000289 DACADA010000000
VERSION     DACADA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.296"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            26..253
                     /locus_tag="ISB17_004730"
     CDS             26..253
                     /locus_tag="ISB17_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982566.1"
                     /translation="MQDPSLRTYRIAFLGSNASGNLPMFTRVQATTGKRAIKAFIERC
                     EPVKGWFLGEPEDITDQLKKEEEEAGSKPQI"
     gene            345..575
                     /locus_tag="ISB17_004731"
     CDS             345..575
                     /locus_tag="ISB17_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982567.1"
                     /translation="MIVSIFSPSAGAVKPRRHSRILRADITAPEIDPALRAFGRHIAR
                     SHRKGRGVHIPAMKNTALGQVLRTLELKRAFN"
     gene            627..1982
                     /locus_tag="ISB17_004732"
     CDS             627..1982
                     /locus_tag="ISB17_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAP1982568.1"
                     /translation="MTPTTAQIMTENTVSQTYRATYSPDDNKLRLYASLRLDEETYSL
                     INKAGFRWAPKQELFVAPAWTPGREDVLLSLAGDIEDEDSTLFDRQEQRAGRFSDYSD
                     RRAVESEQALAHVDSLASAVPLGQPILVGHHSERRARRHAQKIENGMKRAVMLFERAE
                     YWEQRAQASLRHAKYKERPDVRYRRIKKIEAELRKSQKHIARSEKYMTMWRAQTLDLK
                     MALLVSNYDHIHACFTLDKYPRPTEKSQYEGSMSLHSALSEEIITFEQARDIAIRCHE
                     RTISHQQRWVNHYQNRLAYERAMLNENGGVVTRTEEFEPGGQVLSRGEWLTILRVNRS
                     QGEVSSVETPCYRFLGYSGTMKLTPDRITDYKAPTAEEASDAKKAAKRPPIVNYPGEG
                     FREMTKAEWAKLPADYKGVRGAAETETHGAYRFRRCMTHGCTLVNVYITDMKTVEIPK
                     K"
     gene            2030..2593
                     /locus_tag="ISB17_004733"
     CDS             2030..2593
                     /locus_tag="ISB17_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAP1982569.1"
                     /translation="MHHDLKHRIQAMRDKLEGRAPVAEIQGSSQLFVTPAPECRRLVE
                     LADVRETDRILEPSAGTGAILQAIRDTVPRAKCDAVELHAGLVSHLQARFPEVRTWCG
                     DFLEYHPDRRYTRIIMNPPFHRGDDIRHIRRALTLLEPGGILTGICLDGPRQQKALES
                     LADVWEPLPRGTFTYTQVATAILRITV"
     gene            2937..>3058
                     /locus_tag="ISB17_004734"
     CDS             2937..>3058
                     /locus_tag="ISB17_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982570.1"
                     /translation="MSDVKVNGSACCDRPGRAAFTVPAIKLPALCGATPFRARAL"
BASE COUNT          703 a          882 c          879 g          594 t
ORIGIN      
        1 tctttaaaaa tcaggaaaaa gaaaaatgca ggatccttca ttgcgaacgt atcggatcgc
       61 atttctggga agcaatgcca gcggcaatct gccgatgttt acccgggtgc aggccacgac
      121 cggaaaacgg gcgataaaag cgtttattga acgctgtgaa cccgtcaaag gctggtttct
      181 gggcgaaccg gaagacatta ccgatcagct taagaaggag gaagaagaag cgggaagcaa
      241 accgcagata tgaaaaaagc agggtaaacc ctgcttctcc cacgccgtaa aaatcgcacg
      301 aacggttttt ccggcttcgt taactaccat aaggagtcaa ctctatgatc gtttctattt
      361 tttcaccttc cgcaggcgct gtaaagcccc gtcgtcattc gcgcattctg cgcgcagata
      421 tcaccgcacc tgagattgat cccgctctca gagctttcgg gcgtcatatc gccagaagtc
      481 accgcaaagg gcgcggtgtc cacatccccg cgatgaaaaa taccgccctc gggcaggtac
      541 tccgcacgct ggaactgaaa cgcgcattta actgatgagg tgagccgccc cgcgaggggc
      601 ggcaactgca ggaaggagaa aacacaatga caccgactac cgcacagatc atgacggaaa
      661 ataccgtcag ccagacttac cgggcaactt attcacccga tgataataaa ttgcgacttt
      721 acgcatcatt acggctcgat gaagaaacgt attccctgat caacaaagca ggctttcgct
      781 gggcgcccaa acaggaactc tttgtggcgc cagcctggac gccggggcgg gaggatgtcc
      841 tgctttccct cgccggtgat atcgaggacg aggacagcac cttgtttgat cgccaggagc
      901 agcgcgccgg gcggttcagt gactacagtg accggcgggc ggttgaatcg gagcaggcgc
      961 ttgcgcatgt tgattcgctg gcatcggctg tcccgctggg acagccgatc ctggtaggcc
     1021 accacagcga gcgccgcgcg cgccggcatg cgcagaaaat tgagaacggc atgaaacgtg
     1081 cggtgatgct gttcgagcgc gccgaatact gggaacagcg cgcgcaggcc tctctgcggc
     1141 acgcgaaata caaggagcgt ccggatgtgc gttaccgccg tatcaaaaaa atcgaggcgg
     1201 aattacgcaa gtcccaaaag cacattgccc ggtctgaaaa gtatatgacg atgtggcgtg
     1261 cgcagacgct cgatctgaaa atggcgttac tggtcagtaa ttatgatcat attcatgcct
     1321 gcttcacgct cgacaaatac ccccgcccga cagagaaaag ccagtatgag ggctcgatgt
     1381 ctctccattc tgccctgtct gaggagatca ttacttttga acaggcgcgg gatattgcga
     1441 tccgctgtca tgagcgcacg attagccacc aacagcgctg ggttaatcat taccagaacc
     1501 gcctggccta cgaacgcgcg atgcttaatg aaaacggtgg cgttgtcacc cgaacagagg
     1561 aatttgaacc aggcgggcag gtactgagcc ggggagaatg gttaaccatt ctccgggtta
     1621 accggagtca gggggaggtg agttcagtcg aaacgccctg ttatcgcttt ctgggctaca
     1681 gcggcacgat gaaactcacg cccgaccgta tcacggacta caaagccccg acagcggagg
     1741 aagccagcga cgcgaagaaa gcggcgaagc gcccaccgat cgtcaactat ccgggggaag
     1801 ggttccggga aatgacaaag gccgagtggg cgaaattacc cgctgattat aaaggcgtgc
     1861 ggggtgctgc agaaaccgaa acacacggcg cttaccgctt ccgccggtgc atgacgcacg
     1921 gctgtacgct cgtgaatgtg tatatcacag acatgaaaac cgttgaaatt ccgaaaaaat
     1981 gacaggaagg ctccccggca tcttgccggg gagatcaggg agtacattca tgcatcatga
     2041 ccttaaacac cgtattcagg cgatgcggga caaactggag gggcgcgccc ctgtcgctga
     2101 aattcagggg agctcgcagc tttttgtcac ccccgccccg gaatgtcgcc ggctggttga
     2161 gctggctgat gtccgggaaa ccgatcgcat tctggaaccc agcgcaggaa cgggcgccat
     2221 actgcaggcg atcagggata cagtcccccg ggcgaagtgt gacgccgtgg agctgcacgc
     2281 cggattagtc agccacctgc aggcacgttt cccggaggtc cggacctggt gcggggattt
     2341 tctcgaatat catccggatc gccggtatac ccgaatcatc atgaatccgc cgtttcatcg
     2401 cggtgatgat atccggcata tccggcgggc attaactctg cttgagcctg gcgggattct
     2461 gacgggcata tgtctggacg gcccgcgtca gcaaaaggcg ctggagtccc tggcggacgt
     2521 ctgggagccg ttgccgcggg gaacattcac gtatacccag gtagcgaccg ccatcctcag
     2581 aataaccgtc tgatgacacg ttaagccggc ggggcacctg tccccgtctg gccgcgcctc
     2641 gaaagaccgc gcgcctgcgg cgccgggaaa accgggccgc gctgacgcgc cgcccgcatt
     2701 tcctttccgc gtcttccctg tttgctgcct gctgtctgtc tgcttcctct tcttctccct
     2761 gctgtgatgc ctctttttaa cccttaaagg ctttaaaagc acacccacac tggacacctt
     2821 cggctcctgg ccagctacgc tgtccggtcg tcgagccaca ctccgctaac cgcgaaaaga
     2881 cgcggtaagc tccgccgaac ccgctacgcg gtttcgggcc tggcggctag tgtctgatgt
     2941 ctgacgtcaa ggtcaacggc agcgcttgct gcgaccgtcc cggtcgcgcc gctttcaccg
     3001 tgccggcgat aaagctgccg gcactctgcg gggcaacgcc gtttcgcgcc cgcgctct
//
LOCUS       DACADA010000290         3035 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.297, whole genome shotgun sequence.
ACCESSION   DACADA010000290 DACADA010000000
VERSION     DACADA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.297"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            243..1370
                     /locus_tag="ISB17_004735"
     CDS             243..1370
                     /locus_tag="ISB17_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982571.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFDFSIFHAKQPIEILAEHSKTSFH
                     LMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISADIKTNVRDLVDFQSSISHVK
                     LFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDDGLYHYTSDLTGTPLLRLKNT
                     VKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINWCNP"
     gene            1367..1882
                     /locus_tag="ISB17_004736"
     CDS             1367..1882
                     /locus_tag="ISB17_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982572.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            1884..>3035
                     /locus_tag="ISB17_004737"
     CDS             1884..>3035
                     /locus_tag="ISB17_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982573.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRY"
BASE COUNT          891 a          608 c          698 g          838 t
ORIGIN      
        1 tatgaaaaga gataaaaatg ctgatgtcaa aagaggctta tgctcggcac agtggagtga
       61 gctgccaaac tgtcgatgac tgggtagccg gtggggcgga agtagttatg tcccgtagca
      121 aggttaagat ttgctcttgt gtgtggggaa ccttagtcaa ttactttcct ggcgcactgt
      181 gttagatttt gtaaaatttt aaaagactaa agatttaata tcacttctcc atggaggttg
      241 tggtggtaga gaagatgaaa caaaattcaa gttcgacgct tggcgcgctt tctgctccta
      301 caacgtggcc gggtgcgaac gctcggctgc ttgggttagg tgtagggctt ccaatagccc
      361 ccttggatcg tctggcacag tttagcgccg ccgaatttga aaggtttaca ctcgaatggg
      421 cctctgaata cttagctggt cagcaatatg tcaacgaagt acaacaacgt ggcggggcag
      481 gagataaagg cagagatatt gttgtatggc ttgatccatc tgatgctgtt cctcgaagat
      541 ggcacttata tcaatgcaaa cattatgatg cgaatttagg ccttcctaag gcaggcatcg
      601 aagttgcaaa agttatctat tatactttca tcaaggacta cacagtacct acacaatacc
      661 attttgttac acataaaggg gtaacatcgc cattccaaga cctcctagat gatccaacta
      721 aactcaaaga gaaaatgcta tccgagtggg caactttttc aaagcaaatt acatcgaaac
      781 atagtgttga cctgacgcct gaacttgaaa aatatattaa agagtttgat tttagtattt
      841 ttcatgcaaa acagcctatt gagatacttg ccgaacacag taaaacctct tttcatctta
      901 tggtctttgg cgcaccatta attgagcggg atccaccaac taggccccca tcatctgtgg
      961 ctcccattga aactgtatat attgaacaac tcttttcagt tatcagcgct gatatcaaaa
     1021 ccaacgtgcg agatttagtt gattttcaat caagtatcag ccatgtaaaa ctctttgaac
     1081 ggtcccgaat aactttttat tgctcggaag ggctaaagga actcgctagg gaccaaatgg
     1141 cgaatcaaga attctttaat tctttacttg tagagtttga tgatggtctg tatcactaca
     1201 cctctgacct gacagggact ccactccttc ggttaaaaaa taccgtcaag gccgctcaga
     1261 ccctgcaact tggtgcgcac ccacttgcca ttcacgtaac gaataaggat cgtgaaggta
     1321 tatgccacca actagccaat acaaacttga ttaattggtg caatccatga gcgaaatgca
     1381 agtatttgaa ggaaactttg agcagagacc ttttaacact ccgctggagt atggatttcg
     1441 tgctctgttc atattggatg cagcggacaa tgtagcaatg gacctacaaa ggatagtttc
     1501 gtatgactat cttcttgtgc ataccagtga cgtacctagg gggcctaaaa gccttcatcc
     1561 agcagttcct caccggggag cagagcttct tgttaagcga agcgcaattc aggcagggct
     1621 gcatttaatg ctatcaagag aattgattaa ggttgtcttt gctgccgaag gtattttgta
     1681 tcaggctacc aatttaacag gaaggtttgt gcggcttttg atgtcacagt attcaaaaga
     1741 gctcgcagaa cgtgcctctt gggtcacaaa gcaattctat gaatatacgg atgaagaact
     1801 tgcttcatat atatctcaga atgtggggca atggggttct gaattcgacc gattgaccgc
     1861 aattgatcta ctggatttat aacatgcttc gtataagaaa aatactattg cgcggaaaca
     1921 gcgttcaaga cgcttatgtt gatttctata agggtgcgaa tattctagca ggggaatctg
     1981 atactgggaa aagttacctt gtgagttgtc ttgactacat tcttggtgcc gaaaaactca
     2041 aaaaaaagct taaagaggcc accgattaca cccatcttta tgttgaattc gaaaatgatg
     2101 aagggggggt gttaacgctc aagcgcggtc ttgagggggg gaaattagag gcacatgatg
     2161 tcgctattca agacatccat ggagaaggta aaataattgc tcctgtgcgt aaaggaacga
     2221 gtaaggggcc agatgtgacg tccatacttt tcccttttgc ggggatcaaa gaggcgaagc
     2281 ttcgcaagaa tgctcgtggc gaaacgcaac gtttctcgat tcgaacgctc gctcctatat
     2341 ttttggtaga tgaagtatcc attatcgacg aatactcgcc agtaacggga cgttccggtt
     2401 atgacgacac tgctcgaaaa cgcatgtttt catacatcct taccgggcac gatgatggtg
     2461 gtgttaccgt agaggaaaag cctgaaattg taaaagcgag gttgatggcg aagcttgaat
     2521 tcatccagga tttgattcga ccacttgatg aacgtttctc aatttgttcg cccaagtttc
     2581 cattaacgag ctcagcagat gatttatcag atcaattgat tgcacaagca attgatgaag
     2641 ttgagagggc tgcagccgct atttctgact tgctcgaggg aataaaaatg gagacagcgt
     2701 taacgttaaa aatagaatct cagttaatgg gagtatctga aattcaatcg agatatagcc
     2761 tattagaaga acgttaccat tccgacctga agcggttgga ttttatttcc gaaggttctc
     2821 actatttcac ttcacttcag gaagttcctt gttccctttg tggacaaaat ttactacatc
     2881 cacacagtga gaacgctaaa aaattaatga acagtaatga agtccgacgt tcttctttag
     2941 ctgaagctgc aaaaattcat ggataccttg caggcctgca aaaagcgatg agtgatcttg
     3001 accgaagaaa agaagctctg aatatagaca gatat
//
LOCUS       DACADA010000291         3010 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.298, whole genome shotgun sequence.
ACCESSION   DACADA010000291 DACADA010000000
VERSION     DACADA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.298"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..415)
                     /locus_tag="ISB17_004738"
     CDS             complement(<1..415)
                     /locus_tag="ISB17_004738"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="catalyzes the formation of pyruvate and
                     N-acetylmannosamine from N-acetylneuraminic acid; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP1982574.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYN"
     gene            567..704
                     /locus_tag="ISB17_004739"
     CDS             567..704
                     /locus_tag="ISB17_004739"
                     /inference="COORDINATES: protein motif:HMM:NF024407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982575.1"
                     /translation="MQCPVTILCVTLCDYLAPELITHSPEESDTVTLRKRRLPLEMMA
                     W"
     gene            1076..1501
                     /locus_tag="ISB17_004740"
     CDS             1076..1501
                     /locus_tag="ISB17_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1982576.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1498..1848
                     /gene="tnpB"
                     /locus_tag="ISB17_004741"
     CDS             1498..1848
                     /gene="tnpB"
                     /locus_tag="ISB17_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1982577.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            1879..2976
                     /locus_tag="ISB17_004742"
                     /pseudo
     CDS             1879..2976
                     /locus_tag="ISB17_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          790 a          774 c          739 g          707 t
ORIGIN      
        1 tattataaac taccatcgga agtccatctg cagaatcaat aatcttcctg taatggatac
       61 aatgctcttc aaatgaaaaa ggataataaa atggagtaac agcagatata gcatgatatc
      121 cgtatttttt tgcagctttt gcaagtacct cgctttctgc cgtactgatt ccgcccacat
      181 gcgctataag tgtcagtctt ccatctgatt catcggctac tgtttcaaga attttttctc
      241 gctctgcaac attctgaaga aatgcttctc cagtagagcc tccaacatac aatcccttaa
      301 tattttgttc gatattaaaa cggatcagtt ttcgaagact ctccatatcg atttgctggg
      361 attggtcata aggagtgggt aatgctgata tgaccccttt aaattcacat tgcattttta
      421 atcaccttca cttaactgca taatgggata cctagtacac catcaagtac aaagattatc
      481 caacagaaaa gaagcacaat gtgaagatta tcaaaaaaaa accatcagaa agctgtcatg
      541 ccgctcagtt aaggatcggt tgatcgatcc agtgtccagt tactattctc tgcgtaaccc
      601 tctgcgacta tcttgccccg gaactgatta cccacagccc tgaagagtcc gacactgtca
      661 cgctacgcaa acggcgtctt cctctggaaa tgatggcctg gtgaattatc agtatggccc
      721 atgagcgtaa ggaaccttca ccagattgtg aaccggctgg acacatgctg ccaggtagtc
      781 gcccctttgt cgcgccgtga tccatgccca gcagggccag gaagcggggc cgtacgccac
      841 gtgttcacgt aaacagcgca gccctgacat aacaccacgc tgtatccgct ctggtgcaat
      901 atgaacctgc tgaccatcga tggcgtgttc taactaacac aggacacgcc agaacacgac
      961 ataaagcttc actcgccaga tacatggtaa gcgtaaactg accgccgtat gtagccatta
     1021 agccagtatt ggtaacgtag gtgcccacct tttcatcaac tagtggacac ctgttatgga
     1081 acagaaaata ttatccgcag aaccccgcag aacattttca aatgagttta aacttcaaat
     1141 ggttaaactg gcttcacaac taggagcctc tgttgcccgt attgccaggg aacacgatat
     1201 caatgataac ctcctgttca aatggctcag gctctggcag aacgaagggc gtatatcgcg
     1261 gcgtcttccg gtaacaacct cttctgacgc tggcgttgaa ttattacctg ttgagataac
     1321 gcctgatgag cagaaagagc ctatggcggc tcttactccg cttttatcca ctccttcaca
     1381 aagcacagtc agtgccagtt cctgtaaagt tgagttccgc cacggtaaca tgacgctgga
     1441 aaatccttca ccagagctgc tcacggtgtt gatccgtgaa ctgaccggga gggggcgatg
     1501 atctcgctcc catcagacac tcgaatctcg ctggttgccg gcgttactga tatgcgtaaa
     1561 tccttcaacg gtctgggtga acaggtacaa catgtgctgg atgagaaccc cttctccggt
     1621 cacctgttca tcttccgtgg ccgacggagt gacatgatta aaatcctctg ggctgatgct
     1681 gatggtctgt gcctgttcac caaacgcctg gaggaaggcc tgtttatctg gcctgcggtg
     1741 cgtgacggta aggtatccat tacccgctcg cagctggcaa tgctcctcga taagctggac
     1801 tggcgtcagc caaaaacatc ccgtcttaac gcactgacaa tgttgtaaaa atgtcatggc
     1861 cggattataa aaacggtcat gagtcggaaa tacctcattc gtatcactga gctggaaagg
     1921 ctgctctctg agcaggctga agccctccgt cagagagatc tgcaactgag tctggttgaa
     1981 gagacggagg ccttcctgcg ctctgcactg gcacgtgccg aagaaaagat cgaagaggag
     2041 gagcgggaaa tagaatatct gagggcccag atagaaaaac tgcgccggat gctgttcggt
     2101 acccgttctg aaaaactgca gcgtgaggtt gaacaggctg aggcccaact gaaacaacgc
     2161 gaacaggaaa gcgatcgtta cagtgggcgt gaggatgacc cgcaggttcc ccgccagttg
     2221 cgacagtcac gtcatcgtcg tccgttacca gcacatcttc cccgtgaaat acaccgcctg
     2281 gagcctgaag aaagttgttg cccggagtgt ggcagtgagc tggattatct gggtgaagtc
     2341 agtgctgagc agctggaact ggtgagtagc gccctgaaag tgatccgcac tgttcgggta
     2401 aaaaaagcct gtacaaaatg tgactgtata gttgaagcgc cagcgccgtc ccgcccgata
     2461 gcgcgcggca tcgcgggctc cggattactt gcccgcgtgt taacgggaaa atactgcgaa
     2521 cacctgccac tgtatcgtca gagtgaaatc tttgcccgac agggtgccga actgagccgt
     2581 gcattaatct ccaactgggt tgacgcgtgc tgccagttaa tgactccgct aaatgatgcc
     2641 ctgtaccgtt acgtgatgaa cacccgcaag gttcacactg acgacacacc ggtaaaagtg
     2701 ctgacaccgg gcagaaaaaa ggcaaaaaca ggacgcatct ggacgtatgt ccgggatgac
     2761 cggaatgcgg gctcatcaga gccaccggcg gtctggttcg cctactcacc agacaggcag
     2821 ggaaaacatc cggtacaaca ccttcgtccc ttccggggta tcctgcaggc ggatgcattc
     2881 agcggttacg atcggctgtt cagtgccaaa cgtgaaggtg atgcacagac agaagttgcg
     2941 tgctgggtcc atgcccggcg aaaaatacac gatgtatgaa cgacatctct tctgacgaca
     3001 ttttcctgct
//
LOCUS       DACADA010000292         2973 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.300, whole genome shotgun sequence.
ACCESSION   DACADA010000292 DACADA010000000
VERSION     DACADA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.300"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(41..1597)
                     /locus_tag="ISB17_004743"
     CDS             complement(41..1597)
                     /locus_tag="ISB17_004743"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP1982578.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSCYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            1780..2001
                     /locus_tag="ISB17_004744"
     CDS             1780..2001
                     /locus_tag="ISB17_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP1982579.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            2001..2381
                     /locus_tag="ISB17_004745"
     CDS             2001..2381
                     /locus_tag="ISB17_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP1982580.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            2386..2565
                     /locus_tag="ISB17_004746"
     CDS             2386..2565
                     /locus_tag="ISB17_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HAP1982581.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            2593..2871
                     /locus_tag="ISB17_004747"
                     /pseudo
     CDS             2593..2871
                     /locus_tag="ISB17_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
     gene            2885..>2973
                     /locus_tag="ISB17_004748"
     CDS             2885..>2973
                     /locus_tag="ISB17_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1982582.1"
                     /translation="MSFSPDSRLTMKALEMAWETRGKPVGVMF"
BASE COUNT          773 a          680 c          737 g          783 t
ORIGIN      
        1 tcaaccccat ccagcaattt ttccagataa ctcaactcgc tcatttctgc acctcgcagc
       61 cttcaatttc agccacaatc gcatcaatat ctttacgcaa ctggtcgatt ttgctgaccg
      121 tagttttaag ctctgcattt agctcagcaa tattgataat ttcgcggtta tctttcgctt
      181 ctacatagca gctcaccgac aggttatagt cattagcgac aacggtctca aacgcgacag
      241 atttcgccag atgagcaaca tcttccttgc tggcaaatac ctgcataatc tgttcgatat
      301 gggcatcggt caggatattg ttgttggtct cttttttgaa tagttcgctg gcatcaataa
      361 actgaacttt ggtatccgtt ttatgtttag acaacaccag aatattgacg gcaatggtgg
      421 tgccaaagaa caggttcggt gcgagtgaaa tcacggtttc gacatagtta ttgtcaacca
      481 gatactgacg gattttctgc tccgcgccgc cacggtaaaa aatgcccggg aagcagacaa
      541 tcgcagcacg acctttggca gaaagatagt tcagcgcatg taatacaaac gcaaagtcag
      601 ctttggattt gggggccaga acgccagccg gggcaaaacg ttcatcgtta atcagcgtcg
      661 ggtcatcgct gccaatccat ttcaccgaat acggcgggtt agaaacgatg gcatcaaacg
      721 gtttttcatc tctgaagtgc ggttcagtca gcgtattgcc cagcttgata tcaaacttgt
      781 cgtagttgat gttgtgcaaa aacatgttca tacgcgccag gttataggtc gtatggttga
      841 tttcctgacc aaaaaaacct tcttcgatga tatggttatc aaactgtttt ttcgcctgca
      901 acaacagtga gccggaaccc gctgccgggt cgtagatttt gttaacgctg gtctgcccgt
      961 gcatagccag ttgtgcaatc agcttggaga cgtgctgcgg tgtaaagaac tcaccgcctg
     1021 acttaccggc atttgccgca tagttagaaa tcaggaactc ataggcatca ccgaacaggt
     1081 caatctgatg ttcgttgaag tcaccaagtt ttaacccttc aacccctttc agaaccgcag
     1141 ccaggcgggc atttttatct ttaacggtgt tacccaggcg gttactggtg gtatcgaaat
     1201 cagcaaacaa acctttgatg tcagcttctg aaggataacc gtaagcagaa ctttcgatag
     1261 caacgaagat gctgtttaaa tctgcattca atctgtcatt ggtatttgct ttcgcagcta
     1321 cgttgcagaa aagctggctt gggtagatga agtagccttt ggttttgatg gcatcgtctt
     1381 taatgtcatc agtaattacg ctgtcatcca gtttcgcata acagatactg tcatcaccgg
     1441 cttcaatata actggaaaaa ttttcgctga taaaacggta gaaaagtgcg cccagaacgt
     1501 attgcttaaa atcccatcca tcgaccgaac ccctgacatc gttagcaatt tgccagattt
     1561 gacgatgaag ctctgcacgt tgttgaatac ttgtcatttt catccactta tttcaggctt
     1621 atgtaattgg cggtgattct acagcaactt ggatgcttta gcagttcgga cattaggcta
     1681 cgaatgacct gcctagaggt ttgttaagcc gcaaagtgct ggtgctttat gcctgtgaag
     1741 tttataattg tgtacacata acgagtacac gaggtgttta tgcaatccat taacttccgt
     1801 accgcgcgcg gcaacctttc tgaagtgctc aacaatgttg aggccgggga agaggttgaa
     1861 atcacccgca gaggccgtga gccagcagta attgtcagca aggctacttt cgaagcctac
     1921 aaaaaagcgg cgctggatgc tgaatttgca tccctgtttg acaccctgga ctccaccaac
     1981 aaggaactgg ttaaccgata atgaggcata tatcaccgga agaacttatt gcgcttcatg
     2041 atgcgaatat aaaccgctac ggcggcctgc cgggaatgtc tgatccgggc agggcagagg
     2101 ccattatcgg gagagttcag gccagagttg cctacgaaga gatcaccgac cttttcgaag
     2161 tctccgccac ctacctggtg gctacagcga gagggcatat attcaatgat gccaataagc
     2221 gtaccgcgct aaacagtgcg ctgttatttc tacgccgtaa cggggtgcag gtatttgatt
     2281 cacctgaact ggcagacctt accgtagggg ctgcgaccgg agagatatct gtatcttctg
     2341 tcgccgacac gttacgtaga ttgtatggtt ctgcggagta gattaatggc acgcaaatac
     2401 aacaaattgt cccgtgaagc gttaaagatg cttcttgatg gcgtgagtcg ccgcgaggta
     2461 aagcaatacc tggctggtaa gcaaattggt gccaggaccg ctattgctgt gttatgccgt
     2521 caggaaatgg ttgtgcttaa acagagaatg ccgggcagca gataaagccc aatcagtgat
     2581 gaaaggtgtg atgtgaaagc cgtaattact ccctttgtac aaaaagagct tggcgtcgcc
     2641 acattcaaag tggatcagga agtcagaaag ctggtggagg ctggccgtaa atttattatg
     2701 gagccggtac cgcgtgagtt aatcgagcac atggacgacg gcctcgttgt ttccgagcaa
     2761 actatggcaa caaatgaggc gttgcagccg ttttttaaca gcgatgaact gtttcgccgt
     2821 attggtggaa ttgactcgct ggtagcgtgg ttgcgcagga aagaggggca ggtcaggctg
     2881 ggccatgtcg ttctcgccgg acagcaggct taccatgaaa gcactggaaa tggcatggga
     2941 aacccgtggt aagcccgtcg gggtgatgtt cca
//
LOCUS       DACADA010000293         2963 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.301, whole genome shotgun sequence.
ACCESSION   DACADA010000293 DACADA010000000
VERSION     DACADA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.301"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..824)
                     /gene="aph(6)-I"
                     /locus_tag="ISB17_004749"
     CDS             complement(<1..824)
                     /gene="aph(6)-I"
                     /locus_tag="ISB17_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP1982583.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVR"
     gene            complement(824..1627)
                     /locus_tag="ISB17_004750"
                     /note="aph(3'')-Ib"
     CDS             complement(824..1627)
                     /locus_tag="ISB17_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP1982584.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1688..2503)
                     /gene="sul2"
                     /locus_tag="ISB17_004751"
     CDS             complement(1688..2503)
                     /gene="sul2"
                     /locus_tag="ISB17_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1982585.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2527..2604
                     /locus_tag="ISB17_004752"
                     /pseudo
     CDS             2527..2604
                     /locus_tag="ISB17_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(2811..>2963)
                     /locus_tag="ISB17_004753"
     CDS             complement(2811..>2963)
                     /locus_tag="ISB17_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAP1982586.1"
                     /translation="YVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDIT
                     RPKAAG"
BASE COUNT          700 a          850 c          825 g          588 t
ORIGIN      
        1 tgtcgcacct gcttgatcgc ggccgcgata gctagatcgc gttgctcctc ttctccatcc
       61 gcgttccaag ctgcggaaag gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga
      121 tcgacgtcca gcgcacgaga gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga
      181 caaaggtcgt ctctgtcagc cggatcgtag aacatattgg cggcgccaaa gcccacttca
      241 ccgaccagac cgacgggatc tatcaccagc cagccgcgac tggagaacat gatgttttca
      301 tgatgcagat cgccatgtag cccacgcagt tccgaggcat tgctcatcat ttgatcggct
      361 ataatcgccg cgtggacgta gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc
      421 tgaaacaaag ctgcaaagcg atcccggatc gggagaaggg cagaaggcag gggttcctca
      481 gatgcggcat acagcttcgc cattagttcc gctgcaattt cggtcgcctg gtagtcgccg
      541 tgctcggcaa cgatgtgaga gagcattcgc tccccggcat attcgagcaa catcagattg
      601 ttctcacgac cgagcaaccg gactgctccc ctcccattgc gccataccag atagtcggcc
      661 ccgcgcagtt catcagcaat gtcttttata ggtttcaatc ccttgacgat tgcaggagtc
      721 ccgtctggca atgaaacttt ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt
      781 tgcgaaacgt gccaatgagc aggaaaaaca ggcggcatga acatcaaccc caagtcagag
      841 ggtccaatcg cagatagaag gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat
      901 tgaataggac agcgaaggcg cgctctgctt catctggcgc tgcccagttc tcttcggcgt
      961 tagcaatcat gagtgccaaa tcggcatagc gatctgctgt tccgagccgc ccaaggtcga
     1021 tcagacccgt gcattgaaga gttttagggt ccaccatgaa gttcggcatg cagggatcac
     1081 catggcaaac aaccatatcg gtgcgctctt ggtcgagccg caccggtagc tctcgttcga
     1141 cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc gtccggtaag aagtcgggat
     1201 tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa cattcgcgac agcctgcgct
     1261 caaacggaca ttgatcaacc gataggctgt gaacagcgcc aagttgctgc cccattgacg
     1321 gccacgcttt gagcaaatcc gctccagaca gatcagccgc cggtactccc ggaattgccg
     1381 ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt gatgacctcg gggcaagcca
     1441 cacctcgacc tttgagccaa atgaggcggt cacgctctcc agcgagctca ccgcggcggg
     1501 aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg tcgaaaaaca aaatcaccag
     1561 attctccgcc tctgacaggc aaccagtcag aatgcgattc accaaaaaaa atattagttc
     1621 gattcaatgg aggttccttc agttttctga tgaagcgcga atatagagaa atatcccgaa
     1681 tgtgcagtta acgaattctt gcggtttctt tcagcgccgc caataccgcc agcccgtcgc
     1741 gcaaggggcg cggctcgtgt gtgcggatga agtcagctcc acctgcggcg gcggcaagct
     1801 ctgcagcgag tgtcgcggcc ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa
     1861 aggatttgcg cgaaacagac agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga
     1921 accgcgccag caccgagagc gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg
     1981 gatcaaggac aaggcggttg cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt
     2041 caaagaacgc cgcaatgtga tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct
     2101 gcccgtcttg caccgaatgc ataacgacga gtttggcaga tgatttcgcc aattgcggat
     2161 agaacgcagc gtctggaaaa ccgcgaatat cattgagata ggccacacca cgcgacaagg
     2221 cataggcttg cgtcgcgggt tgataactgt cgagcgagac gggaatgcca tctgccttga
     2281 gcgcgtccag caccggcgcg atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt
     2341 cgggattgct ggatgccgga ccgaggtcga tcacatctgc cccctcggcc atcagcttac
     2401 gcgcctgcgc aatggctgcg tctggcgcca gataccggcc tccatcggag aaactgtccg
     2461 aggttatgtt gacgatgccg aaaatgatga gcgatttatt catgggggct tctataataa
     2521 taataatcga gcatgagtat catacggatg ctcgggtcga aagggaatcc ccaggcgagt
     2581 aacctgtttg cggtgatcca ttagctgcag gagcagaaga gcatacatct ggaagcaaag
     2641 ccaggaaagc ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt
     2701 gttaagcctt ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc
     2761 cattgaatat aaaagataaa aatgtcttgt ttacaataga gtgggggggg tcagcctgcc
     2821 gccttgggcc gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc
     2881 gcgaccagct ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca
     2941 ctggcctctg acggccagac ata
//
LOCUS       DACADA010000294         2897 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.302, whole genome shotgun sequence.
ACCESSION   DACADA010000294 DACADA010000000
VERSION     DACADA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.302"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            148..2037
                     /gene="mnmG"
                     /locus_tag="ISB17_004754"
     CDS             148..2037
                     /gene="mnmG"
                     /locus_tag="ISB17_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP1982587.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            2101..2724
                     /gene="rsmG"
                     /locus_tag="ISB17_004755"
     CDS             2101..2724
                     /gene="rsmG"
                     /locus_tag="ISB17_004755"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP1982588.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT          721 a          723 c          756 g          697 t
ORIGIN      
        1 gagatcacaa tagaacagat ctctaaataa atagatcttc tttttaatac ccaggatccc
       61 aggtctttct caagccgaca aagttgagta gaatccacgg cccgggcttc aatccatttt
      121 cataccgcgt tatgcgaggc aatcaccatg ttttatccgg atccttttga cgtcatcatc
      181 attggcgggg gtcatgcagg caccgaggcc gcgatggccg cggcgcgtat gggtcaacag
      241 actctgcttt tgacacacaa tatcgacact ctggggcaga tgagctgcaa cccggcgatc
      301 ggcggtattg ggaagggaca tctggtaaaa gaagtggatg cactcggcgg tctgatggcg
      361 aaagcgatcg atcaggcggg tatccagttt aggatactaa acgcaagcaa gggaccggcg
      421 gttcgcgcta cccgagctca ggcagatcgt gtgctctacc gtcaggcggt acgtacggcg
      481 ctggagaacc aaccgaacct gatgatcttc cagcaggcgg ttgaagatct tattgtcgaa
      541 aacgatcgcg tggtcggtgc cgttacccaa atgggactga agttccgtgc caaagctgtc
      601 gtgctcaccg ttgggacgtt cctcgacggt aaaattcata tcggtctgga taattacagc
      661 ggtggccgtg ctggtgatcc gccgtccatt ccgctttctc gccgtttgcg tgaactgccg
      721 ctgcgcgttg gtcgtctgaa aaccgggaca ccaccgcgta ttgatgctcg aaccatcgac
      781 tttagcgtac tggcgcaaca gcatggcgat aacccaatgc cggtattctc gtttatgggc
      841 aatgcgtccc agcatcccca gcaggtgccg tgttatatca ctcataccaa cgagaaaacc
      901 catgatgtga tccgcagtaa tctcgatcgt agcccaatgt acgcaggggt gatcgaaggt
      961 gtcggcccac gctactgccc gtcgattgaa gacaaagtca tgcgctttgc cgacagaaat
     1021 cagcatcaga tcttccttga accggaaggg ctgacctcta acgaaattta tccgaacggt
     1081 atctccacca gcctgccgtt cgatgtgcag atgcaaatcg tccgctccat gcaggggatg
     1141 gaaaacgcga agatcgtgcg tccgggttat gccattgagt atgacttctt cgatccacgc
     1201 gacctgaaac cgacgctgga gagcaagttt atccaggggc tgttctttgc tggtcagatt
     1261 aacggcacta ccggttacga agaagccgct gcgcaaggtt tgctggctgg tcttaacgct
     1321 gcccgtctgt ctgctgacaa agaaggttgg gctccggcgc gttctcaggc gtatctcggc
     1381 gtactggttg atgacctgtg cactttagga accaaagaac cgtatcgtat gtttacctcg
     1441 cgcgcagaat atcgtctgat gctgcgcgaa gataatgctg atctgcgttt gactgaaatc
     1501 ggtcgtgaac tgggcctggt ggatgacgaa cgttgggcgc gctttaacga gaaacttgag
     1561 aatatcgagc gtgagcgtca gcgtctgaaa tcgacctggg taaccccgtc ggcggaagct
     1621 gcagccgaag tgaatgctca cctgactgcg ccgctttccc gtgaagccag tggtgaagat
     1681 ctgctacgtc gtcctgaaat gacttatgaa aaattaacca cgctgacacc gtttgcccct
     1741 gcgttgacag atgaacaggc tgcggaacag gttgagattc aggttaaata cgaaggttat
     1801 atcgcgcgcc agcaagatga gatcgaaaag cagctgcgta acgagaatac cctgctacca
     1861 gcgacgctgg attaccgcca ggtatccggt ctttctaacg aagtgatcgc caaacttaac
     1921 gatcacaaac cggcctctat tggccaggct tcgcgtattt ctggcgtcac gcctgcggct
     1981 atctccattc tgctggtgtg gctgaaaaaa cagggtatgc tgcgtcgtag cgcataacgc
     2041 attaaaaatg cctggtaagc acccgcttac caggcaacgc atcaagaaca ggtaatcacc
     2101 gtgctcaaca aactctcctt actgctgaaa gacgcaggta tttcgcttac cgatcaccag
     2161 aaaaaccagc ttattgccta cgtgaatatg ctgcataaat ggaataaagc gtacaacctg
     2221 acttcggtcc gcgatcctaa tgagatgctg gtacgccata ttctcgatag cattgtggtg
     2281 gcaccgtatc tgcaaggtga acggtttatc gatgtcggca ccggaccggg actgcctggc
     2341 attccactct ctatcgtgcg tcctgaagct catttcacgt tgttggatag ccttggtaaa
     2401 cgcgtgcgtt tccttcgtca ggtgcaacat gagcttaaac tggagaatat tgagccagta
     2461 cagagcaggg tagaagagtt tccgtcagag ccgccatttg atggcgtaat tagccgcgct
     2521 tttgcctctc tgaacgatat ggtgagctgg tgccaccatc ttcctggtga gcaaggccgt
     2581 ttctacgcgc tgaaagggca aatgccggaa gatgaaatcg ctttgttgcc cgaagaatat
     2641 caggtcgaat cagtggttaa acttcaggtt ccagccctgg atggcgaacg tcatctggtg
     2701 gtgattaaag caaataaaat ttaattttta tcaaaaaaat cataaaaaat tgaccggtta
     2761 gactgttaac aacaaccagg ttttctactg atataactgg ttacatttaa cgccacgttc
     2821 actcttttgt atcaacaaga taacgtggct ttttttggta agcagaaaat aagtcataag
     2881 tgaaaatatc agtctgc
//
LOCUS       DACADA010000295         2874 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.303, whole genome shotgun sequence.
ACCESSION   DACADA010000295 DACADA010000000
VERSION     DACADA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.303"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            35..1249
                     /locus_tag="ISB17_004756"
     CDS             35..1249
                     /locus_tag="ISB17_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982589.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1306..>2874
                     /locus_tag="ISB17_004757"
     CDS             1306..>2874
                     /locus_tag="ISB17_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HAP1982590.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPFYLWQVCDNGDYQS
                     GRVLQSVGCLLPEGCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GITHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLAIGGAGMTTSFFANRALVQETSIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSL"
BASE COUNT          568 a          856 c          855 g          595 t
ORIGIN      
        1 tctggtcgat aatgcaaaat aaagggatgt actgatgcca gtccgactta ataccattcc
       61 cgacgcagaa ccctatcccg taccgccagc ccgaattaag tggctgacag ctttattact
      121 gatgctgtct gtcggtgtgg ccctgacttc attattcgcc agcgatgaac tggcaaaaaa
      181 cggcccgtat ttctgggggc tggcctgtgg tgtgcctgcc tttatctggt cactggttgc
      241 ttcagttcgc tggctgtttt ttatcacgca gtacattcgg actgatgcct ggaataagcg
      301 ccgggaagag gtgattttgc aggaaacgcg ccggggacga cgggcgttgc agatactcag
      361 tttttccgta caaaccgcgc tgaatggtga ttccgttgca gaaactataa cagcatttct
      421 ggcacgtcag caggtgttga atacctacgc tgacttgcgc ggagaagata ctgtgcgtcg
      481 cagcgtcatc cctgtattaa ccaataaacc ggttaccgga cgtctatccg aagttttttc
      541 ccgtctgttc agcgatatca ggctgcaact gacgcggcta cccgatgatt ttcatatcaa
      601 cgtactactg gaaattgatg cgccgctttc aggggcctcc gtgagaacta tctggcagga
      661 cgaatggcaa aaagccggac tcccggaagc ccgtctgttt tcagccccgg agccggggct
      721 ggccgccgtg gacgactggc tggacaacca cattcaggaa aaggccgtac tgctggtgat
      781 ttccgtgcgt ctggaaccga agaacccgga acggacggca gaatccgcca ccgcgctgtt
      841 gctggcaaac cggttgacgc aaaccgcgct gacgccgctg gccctgctgc atcgtcctga
      901 atgtatcact gatacagaga tgatggcgtc aggcattgca caggcgctgg actggatgcc
      961 ggtgcagcca gatgccatat ccggtatgtg gactgccgaa ctggacaggg aacagcgtgc
     1021 cgcgttgtta agcctgaacc agccgtttgc tcaggaagcc ctgatgtacg agctggatgc
     1081 ctttctgggg cgctccggac cggctgcgcc gtggctgtcc gtcgctgtgg caacgctggc
     1141 ggcaatacaa tcacaacacc cccaactgac gctcagcggc gttcagggcg ggcattattc
     1201 atgggcgacc gtggtgtcgc cattcgtttc ccctcaggag gcatcatagt ggttccggga
     1261 ttaaaccgtc tccggccccg ttcggtttca gggcctgcca tcgtcatgac cgtggctgtt
     1321 gtggttgcgc tttgcacgtt tattcaccgt aaaccggcat tgctgggcgt tgaaccgggt
     1381 agctcacagc aaatgtactg gatggctggc gtgattgccg caggcgtggt ctttctgatt
     1441 atcatggggg tttatcatct gctgaaccgc cacgctgccg tgcagaactt tgaactccgc
     1501 agacagcttg ccgccggaga tgactatcgt gcggtgacga caaagaaagc cgacagccgt
     1561 catccggcaa tggtggaact cgccgccttc ctgcgcgacc gctacggccc attctggcgg
     1621 cgtaaaatcc gcctgctgct ggtgaccggc gagcctgaac aggcagaggc catcgcgccg
     1681 gggctgaccg ggcaacactg gctggagggc gaccacacgg tgctgatata cggcggcagg
     1741 ccatcagcgg agcctgatgt cgcgctactg accgccttaa aaaaactgcg ccgcagccgt
     1801 ccgctggacg gcatcatctg gccgctgaca gaagaacaga gccgccagac agcgcaactc
     1861 gacaaaggct ggcgcgaact gataaacggc ggtaagcgac tcggttttca ggccccattc
     1921 tatttgtggc aggtctgtga caacggtgat tatcagtccg ggcgcgtcct gcaaagcgtc
     1981 ggctgcctgc tgccggaagg ctgtaccccg gaacaactgg ctgcaatgct ggaagcacag
     2041 accctaccgc tgacggaaca gggcatgtcg cagctgctga ccgataaccg ccatgatttt
     2101 ctgctgcgtc tggcccatac ccttgcagag cggggtatca cccactggca gaccgtcctg
     2161 aaaccgctgc tggcaggcgg cgcattttct tccctgcgcc tgcgcggcct gatgttcagc
     2221 ccgccgcttg ccgccgtacc ggaggccgca cctcatgcgt ggctgccgtc gccggtctgg
     2281 gcaggcatca ccggcgataa cgcgcggggc cgcgcggtgg gctttccgtg gctgcgtacc
     2341 gcactgatgt ccgctgtctg cgtgctggcg ataggggggg ccggaatgac gacctcgttc
     2401 ttcgccaacc gcgcacttgt tcaggaaacc agcatccaga cggcgcgggc gctcgatacc
     2461 cgcctgccgc tggcagaaca actggtggcg ctgcataccc tgcagggcga actggaacgc
     2521 ctgcaatacc gtatccgcga aggtgcgccg tggtatcagc gttttggccc tgaacgtaac
     2581 cagcaactgc tcgccgccgc ctttcccggc tatgcgcagg cggcaaaccg gctggtgcgc
     2641 gacgtggccg ttgaccatct gcaacagcaa ctgaacgcct ttgtcgccct gccgcccaac
     2701 agtcctcagc gtaccgccac cggtgaacaa cgctataagc agcttaaggc attgctgatg
     2761 acttcccgcc cggaaaaggc cgacgctgcc tttttcagta ccacgctgat ggcagacggc
     2821 ctgcgctatg aaaatatccc ggaaggcgtg cggcagagcg tgttgccgtc actg
//
LOCUS       DACADA010000296         2855 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.304, whole genome shotgun sequence.
ACCESSION   DACADA010000296 DACADA010000000
VERSION     DACADA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.304"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(1..135)
                     /locus_tag="ISB17_004758"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="ISB17_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164058.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            849..1055
                     /locus_tag="ISB17_004759"
     CDS             849..1055
                     /locus_tag="ISB17_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1982591.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLNGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1151..1753
                     /locus_tag="ISB17_004760"
     CDS             1151..1753
                     /locus_tag="ISB17_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982592.1"
                     /translation="MKQQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDNRN"
BASE COUNT          690 a          770 c          653 g          742 t
ORIGIN      
        1 gtcaacggca gagttaaagg cttccagctc cgcaggggta agtttataac gcgtgtggga
       61 aatcactttt tccagcgtct cccgggatga acaacgacgg aactgctgtg gttttaactt
      121 ccattagcct ctcattactt tatgctgcgg ttaacaggat gctgtcagca taccgcacca
      181 gcctggcatt ctggtaccct ccccaaactc attactcccg tgccaccact taaccggtct
      241 ctcagactgt cactcaccag gtcggatacg tttaccgctc agacggatgc ttaatgcact
      301 ctgttcacgt gtgttgcagg tacgttttat atacgcaggt tatacgccag ctatcagaga
      361 tagccctgtg ggtacaacgc aatctgctgc tactgcgttc tcatgcccac tgatcgcact
      421 cacagtgttt atttcacata tatgaactca tgtatttgat ctcatgtgtt tgatctctct
      481 tatatattta taataccaat cagacagtct tatcccacca gtgataactg tcgcctgccc
      541 acaggtcaca ggctgatata ccacaaccgc cttcaaaaac aactctgcaa ttatcagaac
      601 gctagttttc cccttgtctc acgaaagtcc tccctccttt atcgcgtgcg cgtgcggaag
      661 catcgtttcg caacaaccac ccgacatacc ggtacggctc agccaccgca atccccccgt
      721 ttatctcctg acggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
      781 gtgaaggtgg tgcgttgttt tcatcaccaa cgccggttga gattaacaac atccggagga
      841 acattctcat gaccacccca gtttcactga tggatgacca gatggtcgac atggcattta
      901 tcactcaact gaacggcctg acagataagt ggttttacag gctcatcagg gatggggcct
      961 ttccggctcc catcaagctg ggccgcagct cccgctggct gaaaagtgaa gtggaagcct
     1021 ggctacaggc gcgtattgca cagccccgcc cgtaatttct gaccgttatc cgttcacccg
     1081 cagcagacgt aatccccggc ctgccggcgg cattctgctg cctgttatat cccccgtgag
     1141 gaatatgaaa atgaaacaac agtatcagac ccgttacgaa ctgctccacg aaagctacca
     1201 gaaatggctg accggcttca cccggcacgc cgtatcctgg ggcgtgtgtc atccgaatat
     1261 ctactacttc cataatctga cgcccgggtg ggtgtcattc aatggtgaaa aaccggagat
     1321 tgccatcgta ccccagagcc tgcaccggct gatttatggc cctgacaaac gggccacgcc
     1381 gcccctggat gatgatctga tagtgaattt atgcaccagt gaacatctgc tggttcatca
     1441 tccgatgctg gaaggcattc tcctgtctga atgtgaacgt ctgaggcagc gttctctggc
     1501 aaataaactg attagcctct tccgtcagtt tggtggcacg gagttgcgcc tcaaactggt
     1561 ctggctttgc tggcttgatt taatgaccgg aaacagcctt gaagactgga aagagaacct
     1621 gaaacggaaa tcagaaaaag agctggagga atggatcatc aaccgtcaga ggcagagcgc
     1681 agcactgacg gatctgatgg atcagtacgt gctcctggca taccgcacaa cggttgacga
     1741 taaccgcaac tgatgccggt tgcttccgga aggtgtgaca aaatacggaa atgatgatgc
     1801 tcccggcccc gcatgctcac ctgcagagcg tgttcgtcgt catcatactg agccatgacg
     1861 ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca ccaccagagg agccacggac
     1921 agggatgttc tttacagccc gatgcacgcc aggttctcag cagactgaat gtgcaggtgc
     1981 ggttattcac cagaaatggc atgctgcggt acatacccat tctggtgaca atatgtacgt
     2041 ggcgacagcg atcccggcac tgtggaagag actcactcag cctgcaggat attctttccg
     2101 gcatccggga atgtccgcct gaatgcaaac aggatgatat ttgaatccgt aatactgttc
     2161 tttcatcata aataatttat gcagatacag ttaaccgtca cagtaccagg tttgttttcc
     2221 cgggaaataa tatccgtcag actgatgccg gaacatatct gaaacacgtt atgatgtttt
     2281 gttatcagaa atgttgtata ttcttatccg tggcgacagt ttaatgccct ctggtatcac
     2341 ctgataatgc atatcctgcg atgattcgtt atatttacgg gacacgcctc ttttcattgt
     2401 gctccttttc agacttcatt ttccgtaagt gttccccggc ataaaggagg tacctttctg
     2461 gcgtttataa ctctctttat cggtgacgcc ggtaatacag ggaataccac accgacaaac
     2521 gaggtattaa cagacgatat ttcagtgtcc ataatttttc tgtccggtta aataacgtca
     2581 gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc ataccgggtt acaacgtaaa
     2641 aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac atgtttacct ttcaataccc
     2701 cctgtctgca gtctccactc ttccatcctc ttaagctacg tcggccacat gagtgcactt
     2761 ccatgataac aggtattcct gtcagcataa cgaagccggt taccttccct tcctcctgca
     2821 tttcctgtca gaatgactgt acagcgcgca gattg
//
LOCUS       DACADA010000297         2790 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.305, whole genome shotgun sequence.
ACCESSION   DACADA010000297 DACADA010000000
VERSION     DACADA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.305"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..206)
                     /gene="yjdI"
                     /locus_tag="ISB17_004761"
     CDS             complement(<1..206)
                     /gene="yjdI"
                     /locus_tag="ISB17_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAP1982593.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSG"
     gene            387..2018
                     /gene="dcuS"
                     /locus_tag="ISB17_004762"
     CDS             387..2018
                     /gene="dcuS"
                     /locus_tag="ISB17_004762"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAP1982594.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKAQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2015..2734
                     /gene="dcuR"
                     /locus_tag="ISB17_004763"
     CDS             2015..2734
                     /gene="dcuR"
                     /locus_tag="ISB17_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAP1982595.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT          747 a          695 c          677 g          671 t
ORIGIN      
        1 ccgcttgggc acgtatcaat cactttcacc acagtggtga cgtcgacttc atccggcatg
       61 atccacggct ttcgtttgag attaaataac ttgccgttgc cacgtacgca attgccagaa
      121 tgctgacata tcgcagtgtt aaaatagaca tcgatatttt cgccggtata acagcgataa
      181 cccccgtcca gtagcgcctg atccatgacc ttccctctct catcagtatg gttttcacta
      241 taagcatagc cccggaatct tcatacagca ctggcagttt ccgttgccag agtctcttcc
      301 cctatatagc caatcattct tactcccttt gaattacccg cctcatcaga gataatgctt
      361 aagaaatttg tcacacaagg aagctgatga gacattcatt gccctaccgt atgttacgca
      421 aacgtccgat gaaattgagt accacagtga tcttaatggt cagcgcggta ctgttctcgg
      481 tgctattggt ggtgcatctg atttacttct cgcaaatcag tgatatgacg cgagacgggc
      541 tagctaacaa ggcactggca gtagcgcgta ccctcgccga ctcgccggaa atccgtcagg
      601 gcttgcagaa aaaagcgcag gagagtggaa tccaggccat cgcggaagcc gtgcgcaaac
      661 gcaacgatct gctgtttatt gtcgttaccg atatgcaaag tcttcgctac tcgcatcctg
      721 aagcccagcg tattggtcag ccatttaaag gtgatgacat ccttaaagcg ctgaatggcg
      781 aagaaaatgt cgctatcaat cgcggttttc tggcgcaggc tttacgcgta tttaccccca
      841 tctacgatga aaatcataaa caaattggcg tagtggcgat cggccttgag ttaagccgcg
      901 tgacccaaca gatcaatgac agtcgctgga gcattatctg gtcggtacta tttggcatgc
      961 tggtcggact gatcggcaca tgcattctgg ttaaagtact gaaaaaaatc cttttcggcc
     1021 tggaacccta cgaaatctcc acactgtttg agcaacgcca ggccatgttg cagtccatca
     1081 aagaaggcgt cgttgccgtg gacgatcgcg gcgaggtcac cctgatcaac gatgccgcac
     1141 aagaattgct gaattaccgt aagtcgcagg acgatgagaa actgtcgacg ctaagccact
     1201 cgtggtcaca agtggtagat gtctcggaag tgttacgcga cggaacccca cgccgcgacg
     1261 aagagattac gattaaagac cgactattac tgatcaacac cgttccggtg cgcagtaatg
     1321 gcgttatcat cggtgccatt tcaaccttca gggacaaaac tgaagtacgt aaactgatgc
     1381 agcgactcga cggtctggtc aactatgctg acgcacttcg tgaacgatcc cacgaattta
     1441 tgaataaatt gcatgtgatt ctcggattat tgcatctgaa gagttataag cagttggaag
     1501 attacattct caaaacagcc aataactatc aggaagagat tggctctctg ctgggtaaga
     1561 tcaaatctcc ggttatcgct ggttttttaa tcagcaagat taaccgcgcg accgatttag
     1621 gccatacgct gattttaaac agtgaaagcc agctgccaga cagcggtagt gaggatcagg
     1681 tcgcgacgct gattaccacg ttgggaaatc tgatagaaaa cgcgctggag gcattaggac
     1741 cggaacccgg tggcgaaatt agcgtaacat tgcactaccg tcacggctgg ctgcactgcg
     1801 aagttaacga tgatggaccg gggatcgcac ccgacaaaat cgatcacatt tttgacaaag
     1861 gtgtctcgac aaaaggaagc gagcgaggcg tcggtttagc acttgtcaaa caacaggtag
     1921 aaaatctcgg cggcagcatc gccgtggaat cggaacccgg gattttcaca caattttttg
     1981 tccagatacc ctgggacggg gagaggtcga acagatgatc aatgtattaa ttatcgatga
     2041 cgacgcaatg gtcgcggagc tgaatcgccg atacgtggca caaatcccgg gctttcaatg
     2101 ctgtggaacg gcttcgacgc tggagaaagc caaagagatt atcttcaata gcgatacgcc
     2161 tatcgacctg atattgctag atatctatat gcaaaaagag aacgggctcg atttactgcc
     2221 tgtcctgcat aacgcgcgtt gcaaaagtga tgtgattgtc atctcctccg ctgccgatgc
     2281 agcaaccatc aaagattcgc tgcattacgg tgttgtggat tacctgatca aacccttcca
     2341 ggcttcccgc tttgaagagg cgctcaccgg ctggcggcaa aagaaaatgg cgctggaaaa
     2401 acatcagtat tatgaccagg ccgagcttga tcagctaatc cacggcagca gctccaacga
     2461 gcaggatcct cgtcgcttgc caaaaggctt aacgccgcag acgttacgca cgctgtgcca
     2521 gtggattgac gcgcatcagg actatgagtt ttcaaccgac gagctggcta acgaggttaa
     2581 catctcgcgt gtttcctgcc gtaaatacct tatctggctg gtcaactgcc acatcttgtt
     2641 caccagtatc cattatggcg tcacggggcg tccggtttac cgttaccgta ttcaggcaga
     2701 acactactca ctactgaaac aatattgcca ataacggatc gctggttatc tgtaagtaat
     2761 aattattacc gtaatgcaaa atacccctgg
//
LOCUS       DACADA010000298         2682 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.306, whole genome shotgun sequence.
ACCESSION   DACADA010000298 DACADA010000000
VERSION     DACADA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.306"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            57..2570
                     /gene="vgrG"
                     /locus_tag="ISB17_004764"
     CDS             57..2570
                     /gene="vgrG"
                     /locus_tag="ISB17_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1982596.1"
                     /translation="MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIE
                     FTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQV
                     MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQGKVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGV
                     AIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGL
                     KMSGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN"
BASE COUNT          734 a          694 c          753 g          501 t
ORIGIN      
        1 tttttaaaga gaatcatact tggatttatt tttcataaat ataaaggagt acagagatgg
       61 atatcccgac tctgaatttc gaccactcac accacaagct caaaattcgc ggcctgaaaa
      121 gcccggcgga tgtgctgacg tttacgggcc acgaacagct cagctcaccc ttccgctacg
      181 acattgagtt taccagtacc aataaagcaa taacgccgga gtcggtgctg atgcaggacg
      241 gcgcattcag cctgacggca ccgcccgtgc agggaatgcc ggtgcagact gccctgcgca
      301 ccctgcacgg cgtcattacc agctttaaac acctctcatc gtcacaggac gaagcccgtt
      361 acgaagtgcg ccttgaaccc cgtatggcgc tgcttacccg cagccgccag aacgccatct
      421 accagaacca gacggtgccg caaatcgtcg aaaaaatcct gcgcgagcgc catcaaatgc
      481 gcggtcagga cttcgtgttt aacctcaaaa acgaataccc ggcccgtgaa caggtgatgc
      541 agtacggtga ggacgacctg acgtttgtct cacgcctgct gtcggaggtg ggtatctggt
      601 tccgctttgc caccgacgcc cggctgaaaa tcgaagttgt cgagttttat gacgaccaga
      661 gcggctatga gcgcggactg acgctgccgc ttcgtcatcc ttcagggctg tttgatggtg
      721 aaacggaagc cgtatggggg ttgaacagcg cgtacagcgt ggtggaaaaa agcgtctcca
      781 cgcgggacta caactaccgt gaagccacgg cggaaatgac cacaggccag catgacgcca
      841 ccgggggcga taacaccacc tacggcgaag cctaccacta cgctgacaac ttcctgcaaa
      901 aaggcgataa agaggcagcg gaaagcggcg cgttttatgc ccgtatccgc cacgaacgct
      961 atttgaatga acaggccatt ctacagggcc agagcaccag ttcactgctg atgccggggc
     1021 tggagataaa agttcagggt gacgacgcgc cagcggtgtt ccgcaagggc gtactgatta
     1081 ccggcgtgac ggccagcgcg gcgcgtgacc gcagctatga actgacgttt accgccatcc
     1141 cgtactcaga acgctatggc taccgcccgg cgcttatccc ctgccccgtg atggccggca
     1201 cgctcccggc acgggtcacc agcacggtga agaacgacat ctacgcccat atcgacaagg
     1261 acgggcgcta ccgggtcaac ctcgactttg accgcgatac gtggaaaccg ggttatgaaa
     1321 gcctgtgggt acgccagtcg cgcccgtatg cgggagacac ctacggcctg catctgccgc
     1381 tgctggcggg aacggaagtc agcatcgcgt ttgaggaagg caacccggac cgaccttata
     1441 ttgcaggcgt aaaacacgac tcggcacaca ccgaccatgt gaccattcag aactacaagc
     1501 gcaacgtact gcgcaccccg gcgaacaaca aaatccgcct cgatgacgag cgcggcaaag
     1561 agcatatcaa ggtcagtacc gagtatggcg gcaagagcca gctcaacctc ggccatctgg
     1621 tggatgccgg aaaacagcag cgcggggaag gctttgaact gcgtaccgat ctgtggggta
     1681 cagtacgggc aaagaaaggc atctttatca gctccgacgc gcaggacaag gcgcagggta
     1741 aggtgcgcga aatggctccg gcgatggcaa tactggatgg cgcacagtca cagatgaagt
     1801 cgctgtccac tgatgcgcag acggcgaatg ccgatccggc agatctgtca tcacagatag
     1861 cgctgttgca gcagagtgta aaagatctga cgcaggctgc gatcctgcta agcgcaccca
     1921 aaggcgtggc gatcgccagc ggagagcacc tgcaactggc ggcgtcgaaa aacctgatcg
     1981 cgaacgcagg aaatcacgct gatatcggtg tcgtgaagaa tatgtttatc ggcgtgggtc
     2041 aggcgctgag cgtgtttgta cgcaaagcgg gtatcaaact gtttgccaat aaaggggcga
     2101 tctcggtaca ggcacagaac gatctgatgg aactgttggc gcagaaatcg atagagatca
     2161 ccagtacgga agatgagatt aaaatcaccg cgaagaagaa aatcaccctt aacggcggtg
     2221 gcagctacat ccggctggat gcctgtggga tagaggccgg tacgccggga gaatataacg
     2281 ttaaggcggg atattacggg cgtaagccga aggcgaagct gacgccggag ctgatggcat
     2341 ttccggtgat taagtctgag gattttaatc agagctttat tttgctggat gaaaatactg
     2401 ggcaaccact tattaattgg ccctatgaac tagaactgga aagtgggcta aaaatgagtg
     2461 ggattactga tgaaaatgga aatacagaac taatatcctc tgataaggag gaggtcgtta
     2521 atattagtgt gtttgaaccc gatgagtttt tagacgatga aattaattaa gacatatagc
     2581 gagaatataa catggcagat aaatccgaaa agaaaaaaga aaccacaaat aaatcagcaa
     2641 aaaaaacact aacgaagaag agcgaaaaat taactccagc aa
//
LOCUS       DACADA010000299         2616 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.307, whole genome shotgun sequence.
ACCESSION   DACADA010000299 DACADA010000000
VERSION     DACADA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.307"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            118..315
                     /gene="fimB"
                     /locus_tag="ISB17_004765"
                     /pseudo
     CDS             118..315
                     /gene="fimB"
                     /locus_tag="ISB17_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            complement(346..1566)
                     /locus_tag="ISB17_004766"
     CDS             complement(346..1566)
                     /locus_tag="ISB17_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HAP1982597.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISLAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
     gene            complement(1585..2103)
                     /locus_tag="ISB17_004767"
     CDS             complement(1585..2103)
                     /locus_tag="ISB17_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP1982598.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKG
                     TEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSEL
                     SYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRL
                     RKWAKNNNISLK"
     gene            2203..2610
                     /gene="fimB"
                     /locus_tag="ISB17_004768"
                     /pseudo
     CDS             2203..2610
                     /gene="fimB"
                     /locus_tag="ISB17_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
BASE COUNT          683 a          607 c          573 g          753 t
ORIGIN      
        1 cacttacgtt agttattaag caaacgttcg cttttataaa cataatcagg ataaaaatgt
       61 tggattattg ctaacccagc acagctagtg cgcgtctgta attctaaggg aaaaacgatg
      121 aagaataagg ctgataacaa aaaaaggaac ttcctgaccc atagtgaaat cgaatcactc
      181 cttaaagcag caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt
      241 tttattcatg gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt
      301 aaggcaaagt gtatataggg tcttccccga tcatggtggg aagactcagt acgccatatt
      361 cagtttccca tagtggaaca taactcccag tttaaagcgt tcccggtttc ggtatcccct
      421 ggcctttatc ctcagcagtc tgatcttgct gttcagcgcc tccgcatttc cattcgagac
      481 gcgatgacgc attgcattca gtattccgta taaccgtttt ttaatggttt tcgctgcgtt
      541 tttcatcatc ggcacatcac attcagacgc cagcgatatc cactgcaacc agtcattcct
      601 gcgttctgtg ctccaggggc gatcccagat attttttgcc agctctttca gtgtccagca
      661 ctggcttgtc tgttgcatct gttcccgcaa ccacatcagc ttttcctgcc gggactcagt
      721 catccatttg tcgctgtact gccacaggaa gcgggtacct ttggcctgac gacgactttc
      781 aacagggagg tgcggatgtt cattctggcg ggttttatca accacttcgc ccagttgctt
      841 cgccacatgg aagcgatcga aggcgatttt ctcgaccgca ttgggtaaat ggatacgcgc
      901 tgctcttata tagcccgcat tcatgtccat cgacagtgtt ttgatggcca acaactgact
      961 gtcagtaagc gaacggagat agctggcaag actctctgtg ccacgatcat cggttaatgc
     1021 cagtgcccgc ccgtcgcgat cagataccac ggttatatac ctatgccctt ttttaaaggc
     1081 gacttcgtct acattcatat gacgcactga taaaggcttt ttaatccgcg acaggcctcg
     1141 cttaaccgcg cgggtcatga tgccgtcaac ggcattccag ctaagtttaa gttgttttct
     1201 gaccgcatca acggtgctga ttttaagcca tgagagcacg aacgattcga acagcagagt
     1261 gtaccgactg ccggaacctg cccacggtac aggcagagtc tggcagccat gctccgggca
     1321 cataacgcgg ggaacatcgg cttctactaa tgtcatgaac tggcaggtat caaggtggcg
     1381 ccatttacga tgtcggtgat cgtgaacaga acaggatttc ctgcaggtcg gacaggttaa
     1441 ctgagtattt tcagcaattc cgactgtaac agtaacggaa cctgcatttt catcgagggt
     1501 aagggattta acctgccatg gtgcagtcag gttaaggata tgggcataga gggatttttc
     1561 gtccatataa tttcattctg aagcttactt taaactgata ttattatttt ttgcccactt
     1621 tcttaatctt ccattagcgt tggcgtaagg cgaagatgtg ttaaatgaga tcattctccc
     1681 catcgtccat tttgtgtacc atggtcttcc atacaaaata tcatcggtac gatcattaat
     1741 aaggttcaca atctcatttt ttacagtctg aagttcagct actaacaact cataacttaa
     1801 ctcactgtaa tctgagtaaa atttttgagc aagaaggcca agctgattcc atttatagcc
     1861 agtttccgga aaatcgacag gcagaccttt agcatcagaa gcgatccact ttacaacaag
     1921 agcattccat ccaagcagat acgaaacgag atcacgaaca ctcatctccg ttcctttggc
     1981 gtgtccgtcc attgatttat ctgaagtaat ttctggtggg attgtgttga ggtaactaat
     2041 taatttacta aaatttttat caatagctaa aagcagttca gcttttgttt gcggcacact
     2101 cataagaatt ccctctcatg taaaaagaga caccataaag gtactgtgtg gtgtttgaaa
     2161 atcactcaat tatgtaccac ccaccacaac aggggaagac ccatatatat ccatcgatta
     2221 aaaaaaggct tttcaacaac gcatccgcta ttgaacaaag aagttcaggc tttaaaaaac
     2281 tggttgagta tccgtacttc gtacccgcat gctgagagcg agtgggtatt tttatctcgt
     2341 aagggaaatc cgctttctcg gcaacagttt taccatatta tctcgacttc cggtggtaat
     2401 gccgggttgt cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg
     2461 gcgaatatgg gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt
     2521 catactgtct ggtataccgc cagcaatgca gggcgttttt acggcatctg ggatagagcc
     2581 agaggacgac agcgtcacgc tgttttatag agatag
//
LOCUS       DACADA010000300         2608 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.308, whole genome shotgun sequence.
ACCESSION   DACADA010000300 DACADA010000000
VERSION     DACADA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.308"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(56..829)
                     /gene="yaiO"
                     /locus_tag="ISB17_004769"
     CDS             complement(56..829)
                     /gene="yaiO"
                     /locus_tag="ISB17_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HAP1982599.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(831..1412)
                     /locus_tag="ISB17_004770"
     CDS             complement(831..1412)
                     /locus_tag="ISB17_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP1982600.1"
                     /translation="MKNYLKLNAKEKNVQIADQVIIDESTGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPSGTYSLRQELIRTGD"
     gene            complement(1390..2586)
                     /locus_tag="ISB17_004771"
     CDS             complement(1390..2586)
                     /locus_tag="ISB17_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP1982601.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
BASE COUNT          724 a          645 c          591 g          648 t
ORIGIN      
        1 gagttatagc ttttctctga aaactaccga ataaaatata ctgatagact gctgcctaga
       61 atttccagat caggtgggct gcaagttgca gaccgttata atcatcggtt ggggtgttga
      121 accacacttt acctgccgtc aacccgagat taagttgctc agtcagcggt tgaattcgtt
      181 gcaaactgat gctcttcatg ctgccgtagc gtgtttctgg cgtccagtcg taggtgtaag
      241 cacctgttcc gcggcttagc catagttgcg tataaccagc accgcgcggg tccttcagac
      301 gcacggaaat catattgcta taactactac ctgcatcgct ggagtcataa tgggtatagc
      361 ggtaactggt gatcaccggg ccagtataga gtgatacgcc gccttgccag gcattgactt
      421 cgacatcatc gtaatattta gtgtagcgat aaccggtcgt gaaaagcgtt tttggcaaca
      481 gggctaggtt aatatcctgt cgaaaatcct ggcgggcaaa gaccggcgta ttatccgcaa
      541 aggcaatacc cgttcgggtt gtcagccagt tattccattt gtaccagact gcaccctggc
      601 cgcgagtggc attgaaatgt tcagtttcat aatcacgacg cccctgggaa agattaaaaa
      661 gcagcgttgc gttttcaact tttgctacca gttcagcata cgctaaatta cggttgccat
      721 gttcgccaga ataatcggta aaatcgtagc ccgcggtaat ggaagttaac ccggcataag
      781 cgggcaatgc actaaaaaaa aacggcacta ataacgtatg ctttatcatt ttaatctcct
      841 gtacggataa gttcttgtcg gagtgagtaa gttccactag gtaaattacg ttctacaatc
      901 acgcgcgggc caagttgtgt gttcggagaa ataattcgcc caggcaaaat aataacttgt
      961 acgccaaggc gtgaacgctt cccgatataa caacctaatt tatcgcatcc ggtagcgata
     1021 attccctctg gagttcgaac agacacgggt tgttcatcca gacgatgatt actggtacgt
     1081 acttgtgcgc ccaaatatgc ctggtttgca actaccgagt cggcaataaa gcattgcgga
     1141 ccaatcgttg cttccgcttc aataaccgca tttttaattt cggtggcaaa accaattttt
     1201 acgccattgc tgattattgt gccaggacga ataaacgcat aattacctat caggcagttt
     1261 gcgccaatca ctaccggccc ctgaataacg gcaccgtggc aaatacgggt attcgcgccg
     1321 ataacaacct caccggtact ttcatcaata atcacctggt ctgcaatctg aacatttttc
     1381 tctttagcat taagcttcag atagtttttc agttcctgtg acagaaggtt gggaataagc
     1441 ggttgacgct tccaccagtg cggaatagcg ggtgggtttg tcgcgctgag gttcgcgtcc
     1501 agtaaaaaag gcaataagtc cataaataat ccagatggca taagctaata atacatacac
     1561 aacggaaagc ggtgctaaag gcaccaacaa ccagcaacga tgaaaccagg cgctaaatgc
     1621 accaataaca caaactacgc cgacccagat aagcgggaac attgccaaca ccacaccatg
     1681 tggcccggtg gtgatgaatt cattaaacca ggtagtgaga taaataccaa cgccataaag
     1741 cacaaccaac agcataggaa atatactgaa gataccgaag cggctaaata aaagtctttt
     1801 atgcaggcgc atacagaccg cgtagcccac aatccaacgc cgccagcggc gccactcctc
     1861 acgcggactg ttacattcct gtgggtatac gatgcagcga ttcgcctgcc gaatacggta
     1921 gccgtttgcc accaacgtcc aggtgagatc aaggtcttcg actttagtac gatcagagaa
     1981 accgaactta cgcaatacat cagtacggaa catcccgcag gcaccgctga taataaacgg
     2041 tgcaccgccc agaaactgct gtagcgtgcg cttcataaca atcatcggca actttacggt
     2101 ggcgcggata tgtggcagca gacctgcccc ttttaacgcg gtggagggaa ttccgccaac
     2161 ggcatcagcg ccgcgctcaa tttctgccag catatagccc attccatcat tatcaggtgg
     2221 aacgaaagta tctgcatcac ttaaaaatac ttgatcacac gtggcgtatt ttagtccatg
     2281 cattagtgcg ccacctttac cggtgttttt ttgcgtgacg gcaacaaagc ggtcgcccca
     2341 tttgcgtttg acttccgcca tgaccgcttc ggtattgtcc gtggatccgt cgttaacgca
     2401 aattacccgg caaaaataag ggttacgcag cagattatcc agtgactgcg ccagacacgg
     2461 gccttcgtta taagctggta taatggcgtc aatacagcct ttcttttgac agggtttacg
     2521 tcttagcgta cttaaaaacc atagcaacat tgctatggtc atgaagataa ataacaaagt
     2581 tttcatttct attactccgt tacaacac
//
LOCUS       DACADA010000301         2551 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.309, whole genome shotgun sequence.
ACCESSION   DACADA010000301 DACADA010000000
VERSION     DACADA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.309"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(175..1029)
                     /locus_tag="ISB17_004772"
     CDS             complement(175..1029)
                     /locus_tag="ISB17_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1982602.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1256..>2551
                     /locus_tag="ISB17_004773"
     CDS             1256..>2551
                     /locus_tag="ISB17_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP1982603.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSES"
BASE COUNT          725 a          564 c          594 g          668 t
ORIGIN      
        1 gtcgggattt tatttaattg aagcaattta gtttgcattt ttatttaaaa actgagctag
       61 agtccatcaa aaaaatatat aaaacccaaa accaccgtta gaaatagaaa tagttgaaag
      121 gctcacaact cccggaatgc ggattatctt tgccctgatt tctggcggtt ttattcaggg
      181 tgcaagctgt ctcacccttt cccgatattc tcccggagta caaccaaact cgcggacaaa
      241 cgccttgtga aaagatgatt cactggcata gcctactgac tcagcgatca ccacaacagg
      301 gagcatttcc cgggaaaaca tctgggccgc tatttgtaga cgcaactttg ttaatacagc
      361 cagcggcgtg gttccggaaa catcacggaa aagctgggca aaacttgccc gggacatgtg
      421 ggcgatgctg gccagcgatt cgacggtcca ggcgtgtcca ggcatttcca tcatttgctg
      481 tattaccgca ccaagacgtg gatgcagaag caaactgaga atgtttttct ctgtattaac
      541 ctgtgcaatc cactcacgca ccgcgagggt aaagaatgta gcgcagatct ggctacacag
      601 agcatccact cccggcattg ccgatctgga ttcctgttgt aaaaacggga tcgcctccct
      661 cagccagtta tattccacgc tatggttcac tggtgctaca aacagcgttt ccggcaaaga
      721 cgttaaaaaa taacgcgccg aatgttgcag ccgaagagtt ccacagacaa tacaggtcga
      781 ttcattatcg acatgactca ggcgatgagc tgaattttgt ggtagcagga ccacatttcc
      841 cgggcgtaat gtaaaaatct ctcccgtcgg catttccagc ttcgccgctc cttgcgttaa
      901 cgcatgccaa cgaataaccg acaactcccc ggcaccatgt ggaagttgcc agtcacttcc
      961 taacacgcaa ttcttatcga tcgttccttg tggagcgtta agcatcaaca aacggctaag
     1021 agcatccata tcagactcct gagtggtttt tggattacaa acaccaacaa tcttagtcaa
     1081 taattaatct aataccatca aatagttaat catgattttt gttttgtgat tctgattaag
     1141 aaaaagaaaa taccagacga taagacaaaa tatcaagcgt tgtgaagaaa tgttatttgc
     1201 tcttttgcgt ctatagtcat gatgtcaaat gaacgcgttt cgacaggaaa tcatcatgaa
     1261 taaatatcag gcagttatta ttggttttgg caaggctgga aaaacattag ccgtcacgct
     1321 ggcaaaagca ggttggcgtg tggctctcat cgaacaatca aatgcaatgt atggcgggac
     1381 ctgtattaat atcggctgta tcccaaccaa aacattggtt catgacgcac agcagcacac
     1441 agattttgtc cgtgccatac agcgtaaaaa tgaagtggtt aattttttac gtaataagaa
     1501 ttttcataat cttgcggata tgcccaatat cgacgtgatc gacggccagg cggagtttat
     1561 caataatcat agcctgcgtg ttcatcggcc tgagggaaat ctggaaattc atggcgagaa
     1621 aatttttatt aataccggtg cacaaaccgt ggttccgcca attcctggaa ttaccaccac
     1681 gccaggagta tatgacagca ccggattact taatctaaaa gaattgcctg ggcatttagg
     1741 tattttgggc ggcggatata ttggcgttga gttcgcctct atgttcgcta attttggcag
     1801 caaagtaacc attttagagg cagcttcgct gtttttgcct cgggaagatc gggatattgc
     1861 tgataatatc gcgacgattt tacgcgatca gggcgtcgat ataatcctca atgcccatgt
     1921 ggagcgaatc agtcaccatg aaaatcaagt gcaagtgcat agcgaacacg cccaactggc
     1981 ggtggatgca ctgttaatag cttccggtcg tcaaccggct accgcttcgt tacatccaga
     2041 aaatgcaggt atcgcagtaa acgaacgcgg ggcaattgtc gttgacaagc aattacatac
     2101 caccgcagac aatatttggg cgatgggaga tgttaccggc gggctgcaat ttacttatat
     2161 ttcactggat gattaccgca ttgtgcgtga tgagttgctg ggagaaggca gacgtagtac
     2221 tgatgatcgg aaaaatgtgc cttattccgt attcatgaca ccgccactgt ccagggttgg
     2281 tatgacagaa gaacaggcca gagagagtgg tgctgatatt caggtggtga cattgcctgt
     2341 agctgcaatt ccgcgtgcca gagtgatgaa tgatactcgt ggggtattaa aagcgattgt
     2401 tgataataaa acccaacgta tattaggggc atcactgctg tgtgttgact cccacgagat
     2461 gatcaatata gtgaaaatgg tgatggatgc cgggctgcct tatagcatat tacgcgatca
     2521 gatatttact catccgtcga tgagcgaatc a
//
LOCUS       DACADA010000302         2549 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.310, whole genome shotgun sequence.
ACCESSION   DACADA010000302 DACADA010000000
VERSION     DACADA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.310"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            210..2489
                     /gene="ydeP"
                     /locus_tag="ISB17_004774"
     CDS             210..2489
                     /gene="ydeP"
                     /locus_tag="ISB17_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAP1982604.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          677 a          592 c          637 g          643 t
ORIGIN      
        1 ttgagaatta tgttaattta aaccctatga gagatgatgc ttaacttatc gtatttatac
       61 tactctatta tttaacgata atagagtaaa aatagtaaga gagtagtccc ctactcttat
      121 atatccatgt tgacgataaa ccctatgtat atattgtgca tcatcgctat tacaaatcct
      181 aataattcat ttccacacag gataagcaga tgaagaaaaa aattgaatcc taccagggag
      241 ctgccggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata
      301 tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg
      361 gatgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      421 aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata
      481 cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga cgtctcactc
      541 agcctttgaa atatgatgac gtcagcgact gttacaagcc attaagctgg caacaagctt
      601 tcgacgaaat tggcgctcgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      661 cttcggggcg cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg
      721 ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgaccagt gtgggattgg
      781 cagcgagtat cggtgtgggt aaagggaccg tgttgctgga agactttgag aaatgcgatt
      841 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc
      901 gcgctttagt gaaacgggga gcgaaaatga tcgctatcaa ccctctacag gaacgtggcc
      961 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
     1021 tagccagtgc ctactataac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga
     1081 tgcgcctgtt aattgagcgc gatgatgctg caagcgccgc aggtcggccc tcattgctgg
     1141 atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgtgctca
     1201 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1261 ttgctgatgc atatgccgct gccgaacgaa ccattatctg ttacggaatg gggatcactc
     1321 agcacgaaca tggtacccag aacgtacagc aactggtcaa tctgctgttg atgaaaggta
     1381 acattggcaa gcctggtgcg ggtatctgcc cactacgagg tcactctaat gtacagggcg
     1441 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc
     1501 gctatggctt caccccacct catgcacctg gacatgctgc aatagccagc atgcaagcta
     1561 tatgtacggg gcaagctcga gcattgatct gcatgggggg caactttgca ctggcaatgc
     1621 cagatcggga agcgagcgct gtaccgttaa cgcaattaga tctggcggta cacgtagcca
     1681 ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg
     1741 gacgtagcga gattgacatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa
     1801 tgtcgatggt tcatgcctcg cgtggtgtgt taaaacccgc cggtgtaatg ctgaaatcag
     1861 agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg
     1921 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1981 ttgccgacta taaccagcgc atccgtcatc ctggtggttt tcacctgata aacgcagctg
     2041 ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt
     2101 tagaagatcc ttcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg
     2161 atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggtgta ttcggtcaac
     2221 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2281 gagttaatct tattgcactc acgccagacg gtaaacgtag ctcacgccgc atggatagat
     2341 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttc ccagaatcga
     2401 atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc tataaaagta
     2461 ttccggttga attagaacca tcaaattaat tctcctcctc atttcttctg ctgtcatccg
     2521 cacagcagaa gaattcctca ttgactatt
//
LOCUS       DACADA010000303         2503 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.311, whole genome shotgun sequence.
ACCESSION   DACADA010000303 DACADA010000000
VERSION     DACADA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.311"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            174..287
                     /locus_tag="ISB17_004775"
     CDS             174..287
                     /locus_tag="ISB17_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982605.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            378..1504
                     /locus_tag="ISB17_004776"
     CDS             join(378..702,702..1504)
                     /locus_tag="ISB17_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982606.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLWKKLMPLLDKLREQYGVGPVCSEQHIAPSTYYHCQQQRHHPDKRSARCQRDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKTVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWWVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYMERLKEAKLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNR"
     gene            complement(1473..1688)
                     /locus_tag="ISB17_004777"
     CDS             complement(1473..1688)
                     /locus_tag="ISB17_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982607.1"
                     /translation="MCCGHRLIPASVRYDQILLNRPGFPGECFICELRLPDHCFRWKH
                     NKLFLLLPEEYGPAFPAIIDCYTSPPT"
     gene            complement(2155..2388)
                     /locus_tag="ISB17_004778"
     CDS             complement(2155..2388)
                     /locus_tag="ISB17_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1982608.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
BASE COUNT          654 a          550 c          648 g          651 t
ORIGIN      
        1 ggctatcagt ttttgctgat gatcttttta tctatagaaa acatcagcca agctggttgg
       61 ttcagtaacg ctttcatctt tgagcatttt ataaaaaatt cttgaattgt taaaatagat
      121 taaagtctgg taacagcaaa agttaaacga gaaatcaaaa ggaggtccca aatctgaaaa
      181 tgaagcatct gcgtgatgat gttgcagcgg gacgtcgtcg gctgtatatc agagcaatct
      241 gtccatccct gcgtgaagcc ccgttacttt attcgtaccc cttataatgg ggtgttagcc
      301 agccagaccc ggcatgatta ctgtgaaccg ccccgggaat cctggagact aaattccctg
      361 agaaagaggt aaacaggatg actaaaaata cacgtttttc ccccgaagtc cgtcaacggg
      421 cagttcgtat ggttctggaa agtcaggacg aatatgactc acaatgggcg gcaatttgtt
      481 ccatagctcc aaagattggc tgtacgccag agactctgcg tgtctgggtt cgccagcatg
      541 agcgggatac cgggggcggt gatggtgggc tcaccaccgc tgaacgtcag cgtctgaaag
      601 agctggaacg tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc caggcttccg
      661 cttattttgc gaaggcggag ttcgaccgcc tctggaaaaa ataatgccac tgctggataa
      721 gctgcgtgag cagtacgggg tcggaccggt atgcagtgaa cagcatattg ccccgtcaac
      781 gtattaccat tgtcagcagc agcggcatca tcctgataaa cgcagtgccc gttgtcagcg
      841 cgatgactgg ctgaagagag agatacagcg cgtatacgat gaaaatcatc aggtgtacgg
      901 tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc agggtggcca gatgtacagt
      961 ggcgcgcctc atggcggtta tgggacttgc cggtgttctc cggggtaaaa aggtccgcac
     1021 taccgtcagc cggaaaaccg ttgctgcagg tgaccgcgta aaccgtcagt tcgtggcaga
     1081 acgtcctgac cagctgtggg tggctgattt tacttacgtc agcacatggc agggcttcgt
     1141 ctatgtggcg ttcatcattg atgtgtttgc cggatacatc gtggggtggt gggtctcatc
     1201 gtctatggaa acgacattcg tgctggatgc gctggagcag gcgttgtggg cccgccgtcc
     1261 gtccggcacc atccatcaca gcgataaagg ctctcagtat gtgtcactgg cctatatgga
     1321 gcgattaaaa gaagcaaaac tgctggcatc aacagggagt acaggcgact cgtatgacaa
     1381 cgcgatggcg gagagcatca atggtcttta caaagcggag gtaatacacc gtaagagctg
     1441 gaaaaaccgt gcagaagtgg aactggccac actcacgtgg gtggactggt ataacaatcg
     1501 atgattgctg ggaaggctgg gccatactcc tccggcagaa gcagaaaaag cttattatgc
     1561 ttccatcgga aacaatgatc tggcagcctg agttcacaga taaaacactc tccaggaaac
     1621 ccggggcggt tcagtaatat ctgatcgtaa cggacggacg ctggaattaa ccgatgaccg
     1681 cagcacagag agtaggggat gcggacggtc aaagatcgtg gagttggatt ttataaatct
     1741 gtctccagta gtagatgaac tgaatacaaa gttgccagag agtggtctgt caggcaggga
     1801 atcagaaacc ggatattatt gactggtaca ttgtgtcggg ctgtgtacgt tctccccgga
     1861 cagacagtct cagttcgtag aatctataaa ttactgctac tgatgctgcc ggggaaaggt
     1921 gtaacgaaaa agcagcctcc gttaccggac agcaagaagg ctgaatgaag tttatggcgt
     1981 ttgtattttg taatgtctgg ccatgcagta aatccagaca ggtttttatt atcatgtgaa
     2041 gtattctgac ataaaatact aaatttctat tttgtgatga atgctgcaaa actgaatctg
     2101 cactctgatg taacttttat ctgtttcagt gaagcatgcc cacagattga gttattaagt
     2161 tgtggaagaa cagttctgcc ccgcctgctt ctctcctttc agaagccagt atgttgccat
     2221 gcctcgcctt aatggagagc gctgaaccac accttctttt tccagtaata acaggtaata
     2281 gcgtgcctga taatccgtta ccgccagcgc ctccgcaatt tctgcggttt tccctccatt
     2341 atgcctgttc agaaattcca gtatttcatt cttcatatat tcactcatct cactgtaaca
     2401 aagtttcttc gaataataaa aatcatgctc tctgttatca acggaaaggt atttttattc
     2461 tctgtgtttg ctttatttgt gcaatttagt gaatttgctt ttt
//
LOCUS       DACADA010000304         2490 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.312, whole genome shotgun sequence.
ACCESSION   DACADA010000304 DACADA010000000
VERSION     DACADA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.312"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(171..572)
                     /locus_tag="ISB17_004779"
     CDS             complement(171..572)
                     /locus_tag="ISB17_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAP1982609.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            813..1106
                     /locus_tag="ISB17_004780"
     CDS             813..1106
                     /locus_tag="ISB17_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982610.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1178..1837
                     /locus_tag="ISB17_004781"
     CDS             1178..1837
                     /locus_tag="ISB17_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAP1982611.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1914..2375
                     /locus_tag="ISB17_004782"
     CDS             1914..2375
                     /locus_tag="ISB17_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAP1982612.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKGLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          646 a          526 c          637 g          681 t
ORIGIN      
        1 tggatggtaa gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc
       61 gtgagtgatg tcttgtttac attatgttct attcgtcatt aacctcttaa gcgacagaga
      121 ggagtgtggc gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt
      181 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
      241 actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
      301 ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaagag gtaagtatca
      361 gcgccttcat tagagatact cacatgcact ttctgacctt ttttggcata gaaggtatat
      421 gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctactatg gccttttctg
      481 aactcaacat tgacattctt acccgcagca aagctcgtgg aagttagcag tgcaagcaac
      541 aatacagcct tactgatgcc cttaattttc attggtagtc tcctgttcag aatgagtacg
      601 atgaactgtt ataatataac aatccttcac gggagaattc cagtggcttt cctgtgttcg
      661 gttaaatcct ggcgcggcat ttttgccggg ggattactta taaagggggc atggactgaa
      721 caaaccgtag aaatctacag ctggctggct tgtggcgcgg gtttcattga tgttagctta
      781 tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca
      841 aacgtgacca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gttcctgagg
      901 aactgatgaa aggttttggt cagcctcagt tagcgatgat tcttccgctg gatgggcgga
      961 agaaactggt caatgccgat attgaaaagg ttaaacaggc attaaccgag caaggttact
     1021 atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc
     1081 agaaaacaga cgacactaac aaataaccga tatccggcgg tggcattatc tttgtcggcg
     1141 cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca
     1201 aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa
     1261 acatattaaa gagatgggca gcgcagtacc cgaagagcca gtgctgttta ttaaaccaga
     1321 aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca
     1381 tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcta cggaagagca
     1441 tgtccgcaaa gccattgccg gttacggcgt ggcgctcgat ctgacattgc gtgatgttca
     1501 gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggcc aaagcgtttg ataactcttg
     1561 tccgctttcc gggtttattc ccgcggcgga atttaccggc gacccgcaaa atacaacgct
     1621 gggcctgagc gtaaacggcg agcaacgcca gcaaggtacg actgcggaca tgatccataa
     1681 aatcgttccg ctgatcgctt atatgagcaa gttttttacc ctcaaggtcg gtgacgttgt
     1741 gctgacaggc acgcctgatg gtgtcggccc gttgcaaagc ggtgatgagc tgacagtcac
     1801 tttcgatggg cattctttga caactcgcgt tttgtaatac tttttgccgc ctgaaagcgg
     1861 cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg
     1921 aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg
     1981 cagaatggga gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa ctgatggatg
     2041 aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct
     2101 gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt aaattaaccc
     2161 gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgt ttgctggcgg
     2221 aaggtaaagg tttacctgcg tggcatccgc tacttactgg ttcgaaagcg gcaatgcatg
     2281 gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg
     2341 atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg ttgatggggg
     2401 ctttcttaat atacgtctgg atgctcgcca tttcgtagcc ctgtttttga aagcgctctg
     2461 tcataatgag agttacccga tactttatta
//
LOCUS       DACADA010000305         2477 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.313, whole genome shotgun sequence.
ACCESSION   DACADA010000305 DACADA010000000
VERSION     DACADA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.313"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            79..690
                     /gene="ruvA"
                     /locus_tag="ISB17_004783"
     CDS             79..690
                     /gene="ruvA"
                     /locus_tag="ISB17_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP1982613.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            699..1709
                     /gene="ruvB"
                     /locus_tag="ISB17_004784"
     CDS             699..1709
                     /gene="ruvB"
                     /locus_tag="ISB17_004784"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP1982614.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
     gene            1819..2477
                     /locus_tag="ISB17_004785"
                     /pseudo
     CDS             1819..2477
                     /locus_tag="ISB17_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          613 a          594 c          667 g          603 t
ORIGIN      
        1 ttcatccttc gctggatatc tatccagcat ttttttatca tacagcatta tctttgattc
       61 attacgcagg agcgtcatgt gataggcaga ctcagaggca tcatcattga aaaacaaccc
      121 ccgctggtgt taattgaagt gggcggcgta ggctatgaag tgcatatgcc gatgacctgt
      181 ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt tcacccactt tgtggtgcgt
      241 gaagacgcgc aactgctgta cggttttaac aataaacaag agcgcacgtt gttcaaagag
      301 ttgatcaaaa ccaacggcgt cggcccgaag ttggcgctgg cgatcctctc cggaatgtca
      361 gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg gggcactggt gaaactgccg
      421 ggtattggca aaaaaaccgc cgaacgtttg attgtcgaaa tgaaagaccg atttaaaggt
      481 ttgcatggcg atctctttac gccagccgcc gacctggtac tcacgtctcc tgccagcccg
      541 gcgaccgacg atgctgaaca ggaagcggtt gctgcgctgg tggcactggg ctataaacca
      601 caagaagcaa gccgcatggt gagcaaaatc gctcgccctg acgccagcag tgaaacatta
      661 attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat gattgaagca gaccgtctga
      721 tttctgccgg taccactttg ccggaagatg tggcagatcg cgccattcgc cctaaattac
      781 tggaagagta tgttggtcag ccgcaggttc gttcgcagat ggagattttc atcaaagcag
      841 cgaaactgcg cggcgatgcc ctcgatcatc tattgatttt tggtcctccg gggttgggta
      901 aaactacgct tgccaatatt gtcgccaatg aaatgggcgt taatttacgc acgacttctg
      961 gtccggtgct ggaaaaggcg ggcgatctgg cagcaatgct cactaacctt gaaccacacg
     1021 atgtactgtt tattgatgag atccaccgtc tttcaccagt ggtggaagag gtgttgtatc
     1081 cggcaatgga agattaccaa ctggatatca tgattggtga aggtccggcg gcacgctcca
     1141 ttaaaattga tttgccgccg tttaccctga ttggtgcaac cacgcgcgca ggttcgctga
     1201 catcaccgtt gcgcgatcgt tttggtattg tgcaacgtct ggagttttat caggtgccgg
     1261 atctgcaata tatcgtcagt cgcagcgcac gctttatggg gcttgagatg agtgatgacg
     1321 gcgcgctgga agttgctcgt cgtgcgcgcg gtacgccacg tattgccaac cgtctgctgc
     1381 gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac catctcggca gatatcgctg
     1441 ctcaggcgct ggatatgttg aatgtcgatg ctgaaggttt cgattatatg gaccgcaaat
     1501 tgttgctggc ggtaatcgat aagttctttg gcgggccggt aggtctggat aacctggcgg
     1561 cagccattgg cgaagaacgt gaaaccattg aggatgtact ggaaccttat ttgattcagc
     1621 aaggcttttt gcagcgtaca ccgcgtgggc gtatggcgac gacgcgggcg tggaatcact
     1681 ttggcataac gccgccagaa atgtcgtaag tcggattggt cgctcgtgcc gcatccgaca
     1741 ctggcagtgt gcctgatgcg acgctgttga actgcacccc aaatgttgga cataatctga
     1801 catctgaagg tgcagtttat gaaacattca tttgaagtaa aacttgccgc tgtaaatcat
     1861 tacctggctg gtcatgcagg catcatttct acggcaaaac tctttcaact ttctcacacc
     1921 agcctctcac actggattaa tctttttctt cttcacggtc ctcgggcact ggattgcaga
     1981 cacaagcgta gctattctcc tgaagataaa ctttgcgtgg ttctttatgc tctcgggcat
     2041 tctgagtctc taccccgggt agcagcccgg tttaacattc ccagccacaa cacggttaaa
     2101 aactggataa aaggctaccg caaatctggt aatgaggcct ttatcaggcg caggaaggag
     2161 aaaagcatga cccgttctga tgatacccat gaaaacgagg caaacatgac gccggaagag
     2221 atgaagaatg agctgcgtta tcttcgcgca gaaaatgcat atctgaaagc catgcaggag
     2281 catcttctgg aaaaaaagcg ccaggagctg gagaaaaaac gaaagtcatc cagagcctga
     2341 ggtacggaca ctgccagtct gacctgttaa aggccgcagg gctggccaga agcacgctgt
     2401 actaccagct gtcactgcag aaggctaagg acaagtatgc tgatgtgaaa cagctcatag
     2461 cctccatctt ccatgaa
//
LOCUS       DACADA010000306         2442 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.314, whole genome shotgun sequence.
ACCESSION   DACADA010000306 DACADA010000000
VERSION     DACADA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.314"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            53..478
                     /locus_tag="ISB17_004786"
     CDS             53..478
                     /locus_tag="ISB17_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1982615.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            475..825
                     /gene="tnpB"
                     /locus_tag="ISB17_004787"
     CDS             475..825
                     /gene="tnpB"
                     /locus_tag="ISB17_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1982616.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            856..>2442
                     /locus_tag="ISB17_004788"
     CDS             856..>2442
                     /locus_tag="ISB17_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HAP1982617.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLP"
BASE COUNT          621 a          610 c          689 g          522 t
ORIGIN      
        1 agacgagaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
       61 acaggactgg cgaaaagaac ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt
      121 ggaactggca tcacaacctg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
      181 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      241 tcttccggta acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgcc
      301 ggatgagcag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      361 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga cgctggaaaa
      421 tccatcgcca gagctgctca cagtgttgat ccgtgaactg accgggaggg gaagatgatc
      481 tcactcccat caggtacccg tatctggctc gttgccggcg ttaccgatat gcgtaaatcc
      541 ttcaacggac tgggagaaca ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      601 ctgtttatct tccgtggccg acggggtgac accgtcaaaa ttctttgggc tgatgctgat
      661 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      721 gacggcaagg tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg
      781 cgtcagccaa aaacatccag ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      841 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      901 ctctctgagc aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag
      961 acggaagcct tcctgcgctc tgcactgaca cgtgccgaag aaaagatcga agaagatgaa
     1021 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
     1081 cgttctgaaa aactgcgtcg tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa
     1141 caggacagcg atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga
     1201 cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca ccgcctggag
     1261 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
     1321 gctgaacagc tggaactggt gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa
     1381 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1441 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat
     1501 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc
     1561 ttactctcca actgggttga cgcgtgctgc cagttaatga caccggtgaa tgatgccctg
     1621 taccgttatg taatgaacac ccgcaaggtt cacactgatg acacaccggt aaaggtactg
     1681 gcaccgggtc agaaaaaggc gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc
     1741 aatgtgggtt cgtcatctcc tccagcggtc tggttcgcgt actcgccgaa ccggcagggg
     1801 aaacacccgg agcaacacct ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca
     1861 ggttacgaca ggttgttcag tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc
     1921 tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     1981 gaagaagccc tgaagcgaat cagtgaactg tacgccatcg aggatgaaat acggggatta
     2041 ccggagtcag agcgtcttgc cgtcaggcag cagcgaagca aagtgttact gacgtcgctg
     2101 catgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg
     2161 ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga cggtctggcg
     2221 gaggcggata ataatgctgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2281 tttatgttct ttggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2341 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat cctgagcgta
     2401 ctgccggaat ggccttccaa ccgagttgac gaactcctgc ca
//
LOCUS       DACADA010000307         2391 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.315, whole genome shotgun sequence.
ACCESSION   DACADA010000307 DACADA010000000
VERSION     DACADA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.315"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            228..515
                     /locus_tag="ISB17_004789"
     CDS             228..515
                     /locus_tag="ISB17_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982618.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            634..1455
                     /locus_tag="ISB17_004790"
     CDS             634..1455
                     /locus_tag="ISB17_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1982619.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1752..2354)
                     /locus_tag="ISB17_004791"
     CDS             complement(1752..2354)
                     /locus_tag="ISB17_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1982620.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT          647 a          519 c          578 g          647 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaacaga tgatgggggc tgaaaaccag agagaggaga aagcaggatg agtacccgaa
      241 atatccacgt taacaccgcg tcgtacacgc ttcttgttgc cgggaagaaa aaaaacaccg
      301 gggaagagtg ggacgtcctg gaattcagca gcctgactga gctgaaaaaa tatcgcaaaa
      361 gccacccgga aaagatggcc ttcagttata gctacgcgct cagtcagggt gtggataagc
      421 agttccgcca tatcaacatt gcggaagccg atcacttcaa acagttcctg cgtcagatta
      481 agcgtgccgg cctcgatatc cgggcgatct gctgaccgga tgtgatgaat acccctcctg
      541 attgttgcaa tcttgttaat ggagatcggg ggggatgata ttaagcggaa aaatttttta
      601 ttaaatttat tctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg
      661 tataattcca tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct
      721 tcggtatttt ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca
      781 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt
      841 cgggttcgtg atttgggacg tcgcgaatac agtaaacata tgttacgtct tcgcagggaa
      901 gggcatatta acggacagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      961 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
     1021 ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
     1081 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
     1141 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
     1201 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
     1261 cgggaagaca aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata
     1321 aaaggtggat tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca
     1381 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
     1441 aagtggaagt catgataata taagtaaagc cccgaaaaaa ttttcggggc tttacttata
     1501 ttatcatttt tcggcattgc tgtgtcgtct gatatacctc aatttcatca accggctttc
     1561 cggttgtctg taaatattgt gcctgcgaaa aatggtctgg caggaggctg cgcagttacc
     1621 gggtatatat aagatgggtt cccgcttcgc ggggcttcgg ccagtcagta ttgtcactca
     1681 tatgatattt ttgtgtgtgg ccttccatgc cgcttttgcg gcataaccgg tatctgacaa
     1741 tgtctgtaag attaattgtt ctgcacgctg ttaatttccg gaagtgattt cttcgtggcc
     1801 ggaagccgga tacctttact gctttttatc ccggtataaa tccggtaaac atctgatgcg
     1861 taggcaagac gtctctggtt ctggcgttcg gttttcctga atccggcatt gtatgcacca
     1921 acggcctccc aggtgacgcc ccatttttta aatgctattg ccagataata agcaccggta
     1981 taaatgttca tgcagggatc tgttgtcaga tgttctggct taattccgta gcgggccagt
     2041 tcgttaaaat gctgggaatc tacctgcatc agtccgctgc catatcccgt taccggatta
     2101 ataccgatgg cattaacccg gtaacgggat tcttgccatg atattgctct cagtaaatca
     2161 ggatctattt tgtaatcccg gcctgcaaga tcaaagcaat cagtggcttg gcagatctca
     2221 tttataaaca tcaggcagat ggctaacatc cattttttca tttttccacc tctggtgact
     2281 ttatccgtaa ataatttaac ccactccaca aaaaggctca acaggttggt ggttctcacc
     2341 accaaaagca ccacacacta cgcaaaaaca agtttttgct gatttgctac t
//
LOCUS       DACADA010000308         2385 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.316, whole genome shotgun sequence.
ACCESSION   DACADA010000308 DACADA010000000
VERSION     DACADA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.316"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(126..1538)
                     /locus_tag="ISB17_004792"
     CDS             complement(126..1538)
                     /locus_tag="ISB17_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAP1982621.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2095..2304
                     /gene="fumD"
                     /locus_tag="ISB17_004793"
     CDS             2095..2304
                     /gene="fumD"
                     /locus_tag="ISB17_004793"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAP1982622.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          626 a          514 c          583 g          662 t
ORIGIN      
        1 tcttagataa agtgagatta tatagtgaaa tccgatttga ttttttattg cttctggtta
       61 tcgattaaat aaaaaaagcg cccatcagga cgcttcgata tacaaattaa ttcacaaaag
      121 caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac cagtgcacca
      181 gaaaccataa ctacgacatc acctttgtgt gccagaccgc tctgcagagc cagttcttta
      241 cccagacggt agaagtcatc agtagaagtg atctctttaa ccagctgcgg cacaacgcct
      301 ttgctcagta ctaactggtg agccgttttt tcgttggtgg tcagtgccag gatggtggca
      361 tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgagtagc aaccacgatc
      421 agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc ttcggttata
      481 cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg gtcggtacgt
      541 tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt tgcggattca
      601 ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc accggcttct
      661 gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc agtgataacg
      721 actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat aacttcttct
      781 accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg
      841 atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat gtggatgttt
      901 tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg cttacgaata
      961 aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc ctgtttgtct
     1021 ttttcagcca gtgctggcag ggcaatggaa acgccaggca ggttcacacc tttgttttcg
     1081 cccaggtcgc cgttgttcag cactttacag ataactttgt taccttcaat ggcggtaact
     1141 tccataccga tcagaccgtc gtcaaccagt acggtgttgc caacagacag gtcagtagtg
     1201 aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt atcagtagtg
     1261 aaagtaaagg tctgacccgc tttcagagaa acgtcgttac cgccttccag tttcatggtg
     1321 cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt tttgctcatc
     1381 acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg agagaagttc
     1441 agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc agattcggtt
     1501 ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt ctttaagttg
     1561 agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc cggtattacg
     1621 aaagtggttg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg
     1681 tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc tacagtccaa
     1741 agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt aatccaaaag
     1801 gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat tgttatcaaa
     1861 gattcagcgt tccagggaaa aggttacgca aaaacttcgt tgtttgcgca acgtctggtg
     1921 ccggggttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata catcatcgct
     1981 caattgctac agttaaaata atttatcacg ttatttgtct gattttgttt gatttttgtt
     2041 taattggtta gattgatata tgctgatagt tggcatgata aataggtata aagaatggga
     2101 aaccggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg taaagttgtg
     2161 ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg cgtattacgt
     2221 acagcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc gatggaaaca
     2281 gtcattaacg cactagtaaa atgaccattc aggatgaggc ggtaagcttc atcctgatat
     2341 tgttagttca gttgcggtcc agttaatcat tctaaataac aactg
//
LOCUS       DACADA010000309         2374 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.317, whole genome shotgun sequence.
ACCESSION   DACADA010000309 DACADA010000000
VERSION     DACADA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.317"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            251..394
                     /locus_tag="ISB17_004794"
     CDS             251..394
                     /locus_tag="ISB17_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAP1982623.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            407..616
                     /gene="cspE"
                     /locus_tag="ISB17_004795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             407..616
                     /gene="cspE"
                     /locus_tag="ISB17_004795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP1982624.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            782..1591
                     /gene="rlmA"
                     /locus_tag="ISB17_004796"
     CDS             782..1591
                     /gene="rlmA"
                     /locus_tag="ISB17_004796"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAP1982625.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLREWLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1588..2154)
                     /gene="mntP"
                     /locus_tag="ISB17_004797"
     CDS             complement(1588..2154)
                     /gene="mntP"
                     /locus_tag="ISB17_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAP1982626.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          625 a          567 c          580 g          602 t
ORIGIN      
        1 ctcccactgg cctggaacaa ctgaacttat tgaactatgt tagaaaatac gtcagtttaa
       61 gtatctgcct gaactggcaa ggttaagcac aatgatatat cggcgcgtat tccgttgcat
      121 aagtgtgcaa aaaaagtgga agacgtatcg agatttgtgc gtctgatcga gacatgttta
      181 aaaatggctt gccataatta acgttgtatg tgataacaga tttcgggtta aacgaggtac
      241 agttctgttt atgtgtggca ttttcagtaa agaagtcctg agtaaacacg ttgacgttga
      301 ataccgcttc tctgccgagc cttatattgg tgcctcatgc agtaatgtgt cagttttatc
      361 tatgttatgc ctgcgggcga agaaaacaat ctaaggaatt tttcaaatgg caaagattaa
      421 aggtcaggtt aagtggttca acgagtctaa aggttttggc ttcattactc cggctgatgg
      481 cagcaaagat gtgttcgtac acttctccgc tatccagggt aatggtttca aaactctggc
      541 tgaaggtcag aacgttgagt tcgaaattca ggacggtcag aaaggtccgg cagctgttaa
      601 cgtaacagct atctgatcga atccactgat ctgaagtgtg aatacgcttc aatctcgcta
      661 taaagcctcg tcgaatgcga ggctttttac tttgttttat cttcgctcca gtcgttcgga
      721 tatttgcccg ccgcatgatt cgcgttacac ttgcggcctt tagtatcctg ccggagttgt
      781 catgtcgttt tcctgtccac tttgccatca gcctcttttg cgtgaaaaaa acagctatat
      841 ctgtccccag cgacatcagt ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt
      901 tcagcataaa cggtctcgtg atccgggcga cagcgcggaa atgatgcaag cacgccgcgc
      961 attcttagat gccggacatt atcagccgct gcgtgatgca attgtcgccc aactgcggga
     1021 atggcttgat gaaaaggcca cggcggtgct ggatattggc tgtggtgaag ggtattacac
     1081 acacgcattt gctgatgcgt tgcccgaaat caccacgttt ggcctggatg tctcgaaggt
     1141 agcgataaaa gcggcggcga aacgctatcc gcaggtcact ttttgtgtcg cttccagcca
     1201 ccgtttgccg ttttccgata ccagtatgga cgccataata cgtatttacg cgccgtgtaa
     1261 agcagaagaa ttagtgcgag tagtgaagcc cggcggttgg gtcattactg ccacgccggg
     1321 accgagacac ttaatggagc tgaaggggct gatttacaat gaagtacatc ttcatgcacc
     1381 tcatgcagaa caactgaaag gttttacatt acagcagagt gatgagttgt gttatctgat
     1441 gcgtcttcgc ggtgatgaag ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc
     1501 gaagcctgaa gtctggcaag cactggcagc aaaagaagtg ttcgactgcc agacggactt
     1561 taatattcac ctctggcagc gttcttatta accgtggaag tgcgtccaga ggatctggac
     1621 gccaataccg atcagtacca gcccaccgag aatttccgct tttttcccaa taattgagcc
     1681 gataaagcga ccaaccatca tccctaatgt cgacataatc aaggttgcac aaccaatggc
     1741 caatgcggtc gcgataatgt taacctgcag gaaagcaaga ccaacaccca cagccatggc
     1801 atccaggctg gtggcaatcg cggtggttac cagtagccag aaaccgtgtc gacggcgcgg
     1861 ttcttcatct tcatcatctg cgccacgaaa accctcaata atcattcgcc cgccgagaaa
     1921 tatcagcagc acaaacgcaa tccagtggtt ccattcaagg acaaaccgac tggctaacat
     1981 gcccattccc cagccgatca gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt
     2041 tcgcaatgct tcagaaaatt tcggtttatg gagggtggca cctttaccga ttgatgcagc
     2101 aaaggcatcc atcgacatac caaacgcaag aagaacagta gcagtgatat tcatgacaac
     2161 gtcctgaccg gggtatccat aaaacacatc atcgccccca gcaaacatgc atacatgaac
     2221 aacaagccgc gatggttttc gcgttcatgc aggcatagcg attgatgtgc atatggtctc
     2281 gcctgactga aagtgttcag tccgtacgtg ccacgttttg caacgagtat gttgacacgt
     2341 acatttccgg aatctggaaa tcagctactc ccca
//
LOCUS       DACADA010000310         2362 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.318, whole genome shotgun sequence.
ACCESSION   DACADA010000310 DACADA010000000
VERSION     DACADA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.318"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            44..877
                     /gene="xapA"
                     /locus_tag="ISB17_004798"
     CDS             44..877
                     /gene="xapA"
                     /locus_tag="ISB17_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HAP1982627.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            938..2194
                     /gene="xapB"
                     /locus_tag="ISB17_004799"
     CDS             938..2194
                     /gene="xapB"
                     /locus_tag="ISB17_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HAP1982628.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2247..>2362
                     /locus_tag="ISB17_004800"
     CDS             2247..>2362
                     /locus_tag="ISB17_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HAP1982629.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIV"
BASE COUNT          496 a          513 c          635 g          718 t
ORIGIN      
        1 tataaaaagg cgttagattc ccccctacaa aaaaaggata tatatgtatc aggctcagtt
       61 ttctcataac ccactgtatt gcgtagatat tatcaagact tataaacctg atttcacgcc
      121 acgagtggcc tttattttag gttccgggct gggcgcgctg gccgatcaga ttgagaacgc
      181 ggtcgcaatt tcctacgaaa agctgcctgg gttcccggta agtaccgtac acggtcatgc
      241 gggtgagctg gtgctggggt atctccaggg ggtgccagtg gtgtgcatga aaggtcgcgg
      301 acatttctac gaaggtcgtg ggatgaccat catgacggat gcaatccgta cctttaagtt
      361 gctgggctgc gagttgctgt tctgcaccaa tgcggctggc tcactgcgcc ctgaagtggg
      421 ggccggcagt ctggtcgcat tgaaagatca catcaacacc atgccgggaa cgccgatggt
      481 gggtcttaat gatgaacgtt ttggtgagcg cttcttctcg ctggcgaatg cctacgatgc
      541 ggaataccgc gcactgttac aaaaagtggc gaaagaagag ggggtccctc tgacggaggg
      601 cgtgttcgtc tcgtatccgg ggccgaattt cgagactgcg gcggaaattc gcatgatgca
      661 aattattggt ggggatgttg ttggtatgtc tgtggtgcct gaggttattt cagctcgcca
      721 ttgcgaactt aaagtcgttg cggtctctgc gattaccaac atggcggaag gtctgagtga
      781 cgtgaagctt tctcatgccc aaacgctggc agcagcggaa ctctcaaagc aaaactttat
      841 taatcttatt tgcggctttc tgcgcaaaat tgcctgagta aaacaacaat aaacggcctt
      901 actgagtgag gccatatgat gcgacaagga aacgattatg agcatcgctt tgcgcttaaa
      961 ggtaatgtcc tttttgcaat attttatctg ggggagctgg ctggttaccc tcggttctta
     1021 catgattaat acccttcatt tcaccggcgc taatgttggc atggtttaca gttccaaagg
     1081 gatcgccgcg attattatgc ctggtataat ggggatcatc gcagacaaat ggctgcgtgc
     1141 agaacgtgca tacatgctgt gtcacctggt gtgtgcgggc gtactttttt atgcggcatc
     1201 cgtaactgat ccggatatga tgttttgggt gatgttagtc aatgcgatgg cgtttatgcc
     1261 gactattgcg ttatcgaaca gcgtctctta ttcctgtctt gcccaggcag ggcttgaccc
     1321 ggtgaccgct ttcccgccca ttcgcgtttt tggtacggtg gggttcattg tcgccatgtg
     1381 ggcagtaagc ttgctgcatc tggaattgag tagtctgcaa ctgtatatcg cgtccggtgc
     1441 gtcattgctg ctgtcggctt atgcgctgac tttgccgaag attccggttg cggagaaaaa
     1501 agcgactaca tcgcttgcca gcaagctggg tctggatgcc ttcgtgctgt ttaaaaatcc
     1561 acgcatggct atctttttcc tcttcgccat gatgctgggt gcggtgctgc aaattaccaa
     1621 cgtttttggc aacccgttcc tgcatgattt cgcccgtaat cctgagtttg ctgatagctt
     1681 cgtggtgaaa tacccgtcca ttttactgtc agtttcacag atggctgaag tcggtttcat
     1741 tctgacgata ccgttctttt tgaagcgttt cggtattaaa accgtcatgc tgatgagtat
     1801 ggtggcgtgg acgctgcgct ttggcttctt tgcctatggc gatccatcac caaccgggtt
     1861 tattttgctg ttgctgtcga tgattgttta cggttgtgca ttcgatttct tcaatatttc
     1921 tggttcggtc tttgtcgaac aggaagttga ttccagcatt cgtgccagcg cccagggcct
     1981 ttttatgacg atggtaaatg gcgtcggcgc atgggttggc tcgattctga gcggtatggc
     2041 agtggattac ttctcggtgg atggcgtaaa agattggcat accatttggc tgatgtttgc
     2101 agggtatgct cttttcctcg cagtgatatt tttctttggg tttaaatata accatgaccc
     2161 tgaaaagata acgcatcgtt cagttacaca ttaaaaggat tcgcagctct gttccttggg
     2221 gctgctttta tgataaaggt taattagtga aagatatttt attttcttct ggcgttggtt
     2281 ttggcattgg tgctttgttt acaatcgtgc gattacctat tcctgttcca aatgtattgc
     2341 cgggtatatt atccatcgtg tt
//
LOCUS       DACADA010000311         2361 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.319, whole genome shotgun sequence.
ACCESSION   DACADA010000311 DACADA010000000
VERSION     DACADA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.319"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(494..670)
                     /locus_tag="ISB17_004801"
                     /pseudo
     CDS             complement(494..670)
                     /locus_tag="ISB17_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(933..1061)
                     /locus_tag="ISB17_004802"
                     /pseudo
     CDS             complement(933..1061)
                     /locus_tag="ISB17_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1034..1192)
                     /locus_tag="ISB17_004803"
     CDS             complement(1034..1192)
                     /locus_tag="ISB17_004803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982630.1"
                     /translation="MMMSDTTLSRPEVVSGHTDVIYSTSVCHILAVRKSTLLPIDTII
                     RHQVSDIS"
     gene            complement(1292..1468)
                     /locus_tag="ISB17_004804"
     CDS             complement(1292..1468)
                     /locus_tag="ISB17_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1982631.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IAQAREGIRTLRQQ"
     gene            complement(1485..1973)
                     /locus_tag="ISB17_004805"
     CDS             complement(1485..1973)
                     /locus_tag="ISB17_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982632.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGYS
                     LRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GTGYLLRTDTWSYPVLRLKRLGLSKTFRRLVVTLIRCYGVSLIHLDASAGYLPGLPTF
                     DW"
     gene            complement(1970..2347)
                     /locus_tag="ISB17_004806"
     CDS             complement(1970..2347)
                     /locus_tag="ISB17_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP1982633.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGFSACTHSQLINSIDILRARRATG
                     LMIRDNYRTVNNITLGKHPEAKR"
BASE COUNT          590 a          583 c          570 g          618 t
ORIGIN      
        1 caacataatc tacgctcatc cacatgcctg gtgcgtatta ccgtgaagga gatcggtgag
       61 ttattgagtg atctcctttc atgaaaatac gcacctggct ttaactgttc ctgatataag
      121 ttgacagttt tctgtccgta aaacggttga tgaacttcat cgggcgtttt gtatttcaat
      181 gccaaatgtg accgctcatg gttataaatt gccacgaatt cttttaccat ttaccggccc
      241 tacgtcagct ctgcaggacg cgagagtaaa aactcatttt caggattccg tttatccact
      301 cagcctgcgc attctggtgg cagttataac cattagtcat tgaacaggtt attgataaat
      361 aattaaaaaa ctcagttttc tggctcgcct gctcaagctg ttcttcaagt tccctgattc
      421 tttgctcggg tgtcagcggg acagtcgcca gagccacagg cagtctcctc tttacctggt
      481 caggaagtcc ggggctccag tcaagccggc catatttacg caaccagaca agaacggtgg
      541 agcgcccctg aatgccataa ctgcagccgg cctgtttata agtcatctcg cctttttcaa
      601 ccttctcaac aacggctatt ttaaaggata gaggataatc acgttgagtg cgtttgaacc
      661 cagacatcat cacgttctcc gggtttaagt caggaacgtg tcatagctat tcaggacggg
      721 tcaccgacac aaaaaaagtc gcccttgagc gactcgattt gcatacggta tggtgcgaag
      781 gccggactca aacatgcgat ttaacctcat gatttaaaat tgttaataat aaacaacacc
      841 atcctatacc aacacagata ccaacacgaa aaatcactgg ttttcggcat cgaaccagga
      901 caatcaggtc ctgattgaga taccgagctc tgttactcgt catcttcctc gacaggctcc
      961 attgcatcaa gtggtactcg caccatcgcg atagaaccaa tccggataaa agcatcttcc
     1021 ccatctatat cggttaactg atatcgctta cttgatgccg gataattgtg tcgattggca
     1081 acagtgtact ctttcgtaca gccagaatgt ggcagactga ggtggaatag ataacgtccg
     1141 tatgcccgct gaccacctcc gggcgggaga gtgtggtatc tgacatcatc atttttcctt
     1201 tctgtttata aatgaaaatg ccagccgtgt tcaggctgac gtcagggaag tgaaatcggg
     1261 tgagtgatct tcactggttc tggtacaaaa gttactgttg gcgcagggta cggataccct
     1321 ccctggcctg tgcgatacag ggcaacagtg ctgccgaatc tgttttatcc tcatcgttgt
     1381 cgaagataat tcccgattcg cagtcgatat tgtcctgcag ccacgtaatc agaatatcca
     1441 gcgctgtttc cgtggttaat gatttcatgt tgtgaatttc cggattacca gtcgaaagtg
     1501 ggtaaacccg gcagatatcc ggcactggca tccagatgaa tgagactgac accataacac
     1561 cggatgagtg tgacgaccag acgacggaac gtttttgaca gccccaggcg cttaagccgc
     1621 agaaccgggt atgaccacgt atcggtacgt aacagataac cggtaccggt aaaatgaatc
     1681 cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacagcca ggcgttgtcc
     1741 gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggcaacaaa tggtggcgtg
     1801 cagtctgctg aggtccggtc agattcatca acaacacgaa gggagtagcc gttatcgcag
     1861 accatgttaa tcagttcagc gagattaaca ccatcaatgt caacgacaat gcgccccatg
     1921 ttcagcgcct gcacgttaac gctgtcggct tccagcgtca gggccagttt catcgtttcg
     1981 cctccggatg cttccccagg gtaatgttat ttaccgttct gtaattgtcg cggatcatca
     2041 ggcctgttgc ccggcgagcc cggaggatat cgatgctgtt tattaactga gagtgggtac
     2101 aggcgctgaa tcccggcagg tcggtacgta ccagtgcgta tttttccacg agaaagttca
     2161 ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcagcga
     2221 acggtgtgtc attcagcgta aggccgtagt gctggtccag cagtcgggtg agcagtgtct
     2281 gccagatggt gactggagac gggcagcacg atacctcccg tacatgcgtg tcgggtaatg
     2341 ttttcatact gaagattttc c
//
LOCUS       DACADA010000312         2359 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.320, whole genome shotgun sequence.
ACCESSION   DACADA010000312 DACADA010000000
VERSION     DACADA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.320"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..2312)
                     /locus_tag="ISB17_004807"
     CDS             complement(<1..2312)
                     /locus_tag="ISB17_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1982634.1"
                     /translation="MQLSDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIK
                     IDFFAQDNKLNARLTPQQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQK
                     LILNIPQSYLKPQDWLSTPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSS
                     LNLGGWRLRHNGNWSTNSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDS
                     FPFEGAQFSSDDGMIAPELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELR
                     DFNQIFTGDLEVEIREADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNS
                     VSEPQFVQSSAAIGLPYEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQ
                     FSQNYKSFGKQQGQSYRFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVD
                     EKNINSYHQRSRISTTISQDLQDWGQLYLSASKDQYWDTADGYNLSASYSLPFRYISA
                     MLSLGYNKSPYYHEADKSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGS
                     SQDGEFNYAVAQGWQNQNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGIT
                     LHPHGLTLSQPLSLDSASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQI
                     SLDVNSVKDNVELLNTDVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVV
                     TLDSENSINSSIVADQGQVYMSGLPEEDTL"
BASE COUNT          667 a          524 c          496 g          672 t
ORIGIN      
        1 ataagtgtat cctcttcagg taaccctgac atatacacct gtccttgatc agcaacaata
       61 ctgctgttaa tgctattttc agaatctaat gtcaccacac taccaaacgg tacgggctca
      121 ccattcttct gtatcaaggt aattattgcc ttattaccga cattgacttt aaatggagcg
      181 gaaactaaag ctcctcgaga agggatgaca gtaacatctg tatttagcag ctcaacatta
      241 tccttaacac tattaacatc aagtgaaatt tgatttcggt tatagggatt caaatatggg
      301 accacggcat atccacgcca gtccgtataa atacctgacc cgttcaatac ttttacgctg
      361 gcggcatcac gagcctgaac cagagcactg gcactgtcca gacttaatgg ctgggataag
      421 gttaatccat gtggatgcaa cgtaatacca ccgctaattc cgtatgtcca ttgactggat
      481 tctttttgcc aggcatagcc gccgttcatt tgtgcataag gtccacgata gttaaggtta
      541 atgttacctg actctcctct gttttgattc tgccagcctt gcgccacggc atagttaaac
      601 tcaccatcct gtgaggagcc gtttaatcca acctgctgtt gcacctgacc attattcgta
      661 atggtattcg tggttaagaa caggttattt ccattcagca atgagttgag cggtacagac
      721 acagataaaa acagcgattt atcagcctcg tggtaatacg ggcttttatt atacccgagc
      781 gatagcattg ccgagatata gcggaatggc agcgagtaac tcgcagataa gttatatcca
      841 tcagcggtat cccagtactg atctttactt gctgagaggt agagttgtcc ccaatcctga
      901 agatcttgac ttatcgttgt tgatatacgc gaacgttgat gataactgtt tatatttttc
      961 tcatcaacga atccactctg aatttgttgt aactcatcaa aatcgtagta accgcgtgtg
     1021 gcataacgat agccggtaat attcaatgtc gtattcgtct caccaaaacc acgcgaatac
     1081 ataaagcggt aagactgtcc ttgctgtttt ccgaaagact tataattctg cgaaaattgt
     1141 gagcgagcat gagtgacatc cagggagaac gctccaaaaa aatctgagta tttacccagg
     1201 ccaagcagaa ctgctgcata gttgtctgct ttgataccac caccataaaa tgtatattca
     1261 taaggtaatc cgattgctgc tgaagactga acaaactgag gctcagacac actattattc
     1321 aaagtagaac ttgagcgata tttcccgaat gccaggttat agcgtaagcg cccctgacgt
     1381 tgcagaatcg gtaaaacggc cgtcgcctga gtaaaatggc gaatagtgcc atctgcctca
     1441 cggatttcca cctccaggtc accggtgaat atttggttaa aatcgcgtaa ttcaaacggt
     1501 ccaggtggga cgttcttttg ataaatgaca actccgtttt gcttgacgga tacctgtgct
     1561 tgagaataag caatgccacg gacaacagga ctatattggc tgagttctgg ggcaatcatg
     1621 ccatcatcag atgaaaactg tgctccttca aaaggaaaac tgtcaaatat cgcaccatcc
     1681 gttgaggttt gccctacagt aaattgccct ccctgcagga aagaataatc atgctgtaga
     1741 taaacactaa cgggttgcca gtggctctct ttattatatg agtttgttga ccagttaccg
     1801 ttatggcgaa ggcgccatcc accaaggttc agagaactgt ccagagataa aaactgacta
     1861 cggctaccgt agccattatt attttgctta attccgttaa atagatagtt aaccattagc
     1921 gctggcatac cttcatccca gagatggggc ggagtactta accaatcctg aggtttgagg
     1981 tagctttgtg gtatattcaa tattagcttt tggttgttcg catcgaattt ctctgttgcg
     2041 ccctgaataa tatatgcaat attttgctgg taagccgatg gggaagatac ggagagctta
     2101 tttgtatcaa ttccccaacg ttccaactgt tgaggcgtca gtctggcgtt cagcttgttg
     2161 tcctgcgcga aaaaatctat cttaatctga cggtcaacta attccccatt cataactaca
     2221 tcaacagtat attcccctgg agctgcaccg cttgcgtttg caaaataatt gagatcgata
     2281 tttttggcat tatcaccatc agataattgc aaaaaatcgg ggttaaatga gaacctatga
     2341 ctattgtcag gtgagggag
//
LOCUS       DACADA010000313         2357 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.321, whole genome shotgun sequence.
ACCESSION   DACADA010000313 DACADA010000000
VERSION     DACADA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.321"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..429)
                     /locus_tag="ISB17_004808"
     CDS             complement(<1..429)
                     /locus_tag="ISB17_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP1982635.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAA"
     gene            complement(481..2163)
                     /locus_tag="ISB17_004809"
     CDS             complement(481..2163)
                     /locus_tag="ISB17_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP1982636.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          637 a          483 c          519 g          718 t
ORIGIN      
        1 ggctgctatc acttttgcaa aagtaccgtt acctgaatta ctgcgtctgt aacggtcatg
       61 tagttcttgg gggccatcga tcgagatacc aaccagaaac tcatgttctt tgagaaaggc
      121 acaccattca ttattcaata aaatgccatt cgtttgtaat gcattaaaaa tacgtttttg
      181 gcctgcatag cgttgttgat agtgaataac tttacggaaa aaatccaggc cagccagagt
      241 gggttcaccg ccttgccagg taaaatagac ctgattgcca gacgctgcga tatattgttt
      301 gatgaactct ttcagagtgc tgtcatccat ccatttttca tgagtaaact gcgactcttt
      361 ttcaaggtaa aaacagtaat cacatttgag attacattga aaactggagg gcttggctgt
      421 aacgtgcatc gctatctcgc tcaataaggc ggcggaaaaa tccgccgcat aaaggtttag
      481 ttatttcgct tcgcttagtg ctttcttgat attgttaaac ttctcctgat ttacctcgct
      541 aagcggtggc tggctgttat cgataaactc tcttaccacg ccttgcatct ctttaacgac
      601 ctgcggattg gcggcggcaa ggttatcttt ttgctgtaga tccgtcagtt tgtatagacc
      661 taactgatta ttttctactg tatagacaag cgaataatcg ttatttctca ccgtataaga
      721 gaattggctt aagtcctcgg tgttggggtt atgcgggtaa tcgtctgact gatggcggac
      781 aaatttgtgg taattatccc agaatggaat attttcctca tcaaaccagt gagaataaga
      841 tgttatccag gtcagatttt tatgtggctc gccttgtttc ttattttgca accagggcag
      901 caaggaaacg ccatccagct taaggtcttt tggaatgctg atatcggctg catcaagagc
      961 tgtcgggtag aaatccattg cggaaatcag cttgtcataa ttaccgggtt gaagttttcc
     1021 tttccaccac ataaacattg gggtgtgagt accgccagga taggtctgac tcttatagcc
     1081 tttttgcgcc ccgttcagcg gcagaggacc atcgataact gcaccattat cggaggtaaa
     1141 gagaataatt gtattgtcat actgtccgtt tttcttcagt tgttcgagaa tgcgttttac
     1201 accctgatca acagaataaa cggaagcgta gtaattatct gctgtttgac taccggtatt
     1261 aaattgcttc tgatattgct ccggagcagg attatcattt ggcaggtgcg gagcattata
     1321 agccaggtaa agcataaaag gctggtcaag tgttttggca cgatcaacaa cgccaattgc
     1381 ttcatcggtt aactgatcgc tgatataacc ttttgcgggg acgcgttcac ggtttttgaa
     1441 cagtgaaggg gagttgtaat atgccgttcc tgcagcatgg aatcccataa agtaatcaaa
     1501 gccacggttt tgcggttgcc attcttccgc agaaaaggta gtgaagttgt catgatagtc
     1561 acgcgtttgt ttatcttccg gcaccggcac attactgatt tttgataagt gccatttacc
     1621 tactgctgca gtgtaataac catgattctg gaataattca ggcaagaaag tttctgttag
     1681 cggaatacca tcctgagcat cagtattgga atagacacca aagcgggcgg gcgctcgacc
     1741 ggtcattatt gcggcgcggg aggggccgga aacaccgtgc gccacatagc cgttagtaaa
     1801 acgtacgcct tcatccatta atgaaaggag cgtcggcgtt gatttttgcg cagcttcaat
     1861 ggctttatct atccctattt tgtaggtatc gacaacttca cgattttcca ttgtttttgg
     1921 gtcaaaagat cccttatcaa aaggaagttg tccataacca agatcatcca tggtcagtac
     1981 gataatattt ggctttcctt tggtactgta ttcagtcggc gtaaagtctg agaacgcaac
     2041 gtttgttttg gttgctttca gctttacatc atctgccgca tgagcagcaa atgcagccat
     2101 accagatgcc agtatcaaag atatcgaggt acttacgaca cttttcttta atgcagactt
     2161 cataaatgtt cctcttctta ttgtacttaa ttccaaagta attttacgaa gagaaataag
     2221 tgaatgtaag tgaagttagt cacataaaga gatagcagat ttagctaaaa aaagggaaat
     2281 aacagtccat aacgcgttga cattactttc tgttctacta agtaatttct cgccgataaa
     2341 caactaatct attgata
//
LOCUS       DACADA010000314         2342 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.322, whole genome shotgun sequence.
ACCESSION   DACADA010000314 DACADA010000000
VERSION     DACADA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.322"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            105..1076
                     /locus_tag="ISB17_004810"
     CDS             105..1076
                     /locus_tag="ISB17_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982637.1"
                     /translation="MLEKTINNAICTLLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGSMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEYLD"
     gene            1124..2137
                     /gene="yhjD"
                     /locus_tag="ISB17_004811"
     CDS             1124..2137
                     /gene="yhjD"
                     /locus_tag="ISB17_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAP1982638.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT          554 a          612 c          584 g          592 t
ORIGIN      
        1 cgctctgttt aagggaattt acctttctca taaagctatt cctccctttt cctgctgaga
       61 aatcgccaca ttcatcatga caacattgtg aaacccggca ttagatgtta gaaaaaacaa
      121 taaacaatgc gatatgcacg ctactgtttc gttgtgaaca acaatcggtc aaagaaatgg
      181 ataaaattca cgcaatgcag ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg
      241 cggcggattt ctttgctttg ccaaagggaa gtgtttcgcg ccagatacag gcactggaac
      301 atcaacttgg cacccagctt ctccagcgca ccacgcgacg ggtcaaactc acgccagaag
      361 gcatgaccta ttatcaacga gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc
      421 tgtttcaaca ggatgccacc agtatcagcg gtaaattacg catcgacatc ccgtcaggaa
      481 tcgcgaaaag cctgttactg ccgcgcttgt cggaatttct ctatctgcat ccgggaattg
      541 agctggaact gagtagccat gaccgtccgg tagatattct tcatgatggt tttgattgcg
      601 tgatacgcac tggcgcatta ccggaagatg gcgttatcgc ccgtcccctc ggtaaactga
      661 ccatggtcaa ctgtgccagt ccgcactatc tgacgcgctt tggttatccg caaagccccg
      721 atgatctgac ttcacacgca atagtgcgtt atacaccgca cctgggtgta catccgttag
      781 gttttgaggt tgccagcgtt aatggcgtcc agtggtttaa gtctggcagc atgttgacgg
      841 taaacagtag cgaaaactat ctcgccgccg gtcttgccgg tctggggatt attcagatcc
      901 cgcgcattgc cgtgcgcgaa gccctgcgtg ccgggcggct tattgaagtg ttgcctggct
      961 accgtgccga gccgctgtcc ctttcactgg tttatccgca gcgtcgggag ctttcccggc
     1021 gtgtaaacct gtttatgcag tggctggctg gcgtaatgaa agagtacctg gactgaccga
     1081 ctatactttt aagaatgacc acgacagaag gacaaaagag cggatgacgc aggaaaacga
     1141 gatcaaacgt cccacccagg atctggagca cgagccgatt aagcagttgg ataatagcga
     1201 gaaaggcggc aaagttagcc aggcgctgga aaccgtcacc accactgccg agaaagtcca
     1261 acgccaaccg gttattgcgc acctgattcg cgcgacagaa cgctttaacg atcggctggg
     1321 taaccagttt ggtgcggcta tcacctattt ctcatttttg tcgatgatcc ccattttgat
     1381 ggtgtcgttt gccgcagggg gctttgtgct cgcctcccat ccgatgttgc tacaggatat
     1441 cttcgacaaa attctgcaaa acatcagcga tccgacgcta gccgccacat tgaaaaacac
     1501 catcaacacc gccgttcagc agcgtacgac tgtagggctt gtcggtctgg cagtggcact
     1561 ttattccggc atcaactgga tgggtaacct gcgtgaagcg attcgcgccc agtcacgcga
     1621 tgtctgggaa cgctcgccgc aagatcagga gaagttctgg gtaaaatatc tgcgtgattt
     1681 tatttcgctg attggtttgt tgattgcgct gattgtgacg ctttctatca cctcggttgc
     1741 cggttctgcg cagcaaatga ttattagcgc cctgcacctg aacagcattg agtggctgaa
     1801 accgacatgg cggttgattg gcgtggcgat ttccatcttc gccaactatc tgcttttctt
     1861 ctggatcttc tggcggttgc cgcgccaccg tccgcgcaaa aaagccctga tccgcggaac
     1921 atttctggct gctattggtt ttgaagtgat taaaatcgtg atgacctaca ccctgccatc
     1981 gttgatgaaa tcaccctctg gcgcagcatt tggttccgtg ctggggctga tggcattttt
     2041 ctacttcttc gcccgtttga cgctgttttg cgcggcgtgg attgccaccg ccgaatataa
     2101 agacgacccg cgaatgccgg ggaaaacaca gccttaaaat aggccggatg cggcgtacga
     2161 atttatccgg cctggctccc cgtaggccgg ataagatgcg ccagcatcgc atccggctat
     2221 aatgcgcaca taacctcttg aaactctttc ccagagcctc tttagccatc tattcaggag
     2281 caaacaattt cattccaact cataacccca gcatataaat ccagttggta acttttattt
     2341 aa
//
LOCUS       DACADA010000315         2295 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.323, whole genome shotgun sequence.
ACCESSION   DACADA010000315 DACADA010000000
VERSION     DACADA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.323"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            78..581
                     /locus_tag="ISB17_004812"
     CDS             78..581
                     /locus_tag="ISB17_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1982639.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(627..1043)
                     /gene="rraB"
                     /locus_tag="ISB17_004813"
     CDS             complement(627..1043)
                     /gene="rraB"
                     /locus_tag="ISB17_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAP1982640.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1205..2209
                     /gene="argF"
                     /locus_tag="ISB17_004814"
     CDS             1205..2209
                     /gene="argF"
                     /locus_tag="ISB17_004814"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP1982641.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          587 a          542 c          575 g          591 t
ORIGIN      
        1 tataagacgc ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat
       61 ttttattggg ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga
      121 cgttgcagga taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc
      181 ttaccgctga taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt
      241 atagtcagcc tggtcatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg
      301 gtgggattgc gccattagca ggtagtgaat cggacatttg cgaactgcaa aagatgtatt
      361 ttcttccggc tattcgcggt aaagggctgg cgaaaaaact ggccttaatg gcgatggagc
      421 aagcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag
      481 ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg
      541 gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtgtg
      601 tgctatcggg cattttaatc aattaattag tggcgaacac cgtcatcgtc ttcatcgaca
      661 aaatcttcat cgtcgccgtc ttcgccattg ggatcttcaa agtaagtgcc ccaaccgtcg
      721 tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga
      781 tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca
      841 acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgctgct
      901 ttttccaggg tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc
      961 ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct
     1021 tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt
     1081 gtcacacacc actgacattg cctccacctt tgtgacaaag atttatgctt tagacttgca
     1141 aatgaataat catccatata aattgaattt taattcattg aggcattagc cacaggaggg
     1201 atctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga
     1261 actcaacacc ttgttgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga
     1321 agaagctaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgactcgtac
     1381 ccgatgctct ttcgaagttg ccgcatatga ccagggtgct cgtgttactt atctcggccc
     1441 aagcggcagc cagattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg
     1501 catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga
     1561 atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct
     1621 ggcggatctc ctcaccatgc aggagcattt gcccggtaaa acattcaacg aaatgacgct
     1681 ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcggcgct
     1741 taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt
     1801 tacggaatgc cgcgctctgg cacagcaaaa tggcgggaat attacgctga cggaagatgt
     1861 agccaaagga gtagaaggtg ctgactttat ctataccgat gtgtgggtgt cgatggggga
     1921 agcaaaagag aaatgggcgg aacgaattgc attgctgcgt gattatcagg tgaataacaa
     1981 gatgatgcag ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcatttca
     2041 tgacgaccaa acgacgcttg gcaagaaaat ggcggaagag tttggcctac atggcggaat
     2101 ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa
     2161 ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct
     2221 ataaagatgc cgggaaatac aaaactatcc cggcatcaca ttattttatg cagtgatagg
     2281 tctattcttc caccc
//
LOCUS       DACADA010000316         2291 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.324, whole genome shotgun sequence.
ACCESSION   DACADA010000316 DACADA010000000
VERSION     DACADA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.324"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..330)
                     /locus_tag="ISB17_004815"
     CDS             complement(<1..330)
                     /locus_tag="ISB17_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982642.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     PFYINVTG"
     gene            complement(361..720)
                     /locus_tag="ISB17_004816"
     CDS             complement(361..720)
                     /locus_tag="ISB17_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1982643.1"
                     /translation="MNRKLLALFMAAITLPVAAAQYVKPGAVLTLHPGQNNAEFNINA
                     STGDGSGVCNMEGTAQQIAAGESQKRRWVWNDNASECVAVISELTNGKASIMTRGCEG
                     YCGASAAGAMDGLFNKK"
     gene            complement(735..1262)
                     /locus_tag="ISB17_004817"
     CDS             complement(735..1262)
                     /locus_tag="ISB17_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982644.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(1274..1543)
                     /locus_tag="ISB17_004818"
     CDS             complement(1274..1543)
                     /locus_tag="ISB17_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1982645.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
     gene            1797..2153
                     /locus_tag="ISB17_004819"
     CDS             1797..2153
                     /locus_tag="ISB17_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006324788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982646.1"
                     /translation="MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGE
                     AFTLNDSDRQLSLWVQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKL
                     PEDERKRLFEKLNGKV"
BASE COUNT          581 a          551 c          515 g          644 t
ORIGIN      
        1 acctgttacg tttatataaa acggaccttt accctcatca gttttctgaa tccggtaggt
       61 gattggtaga gaattttgca aatctggttt tgtaaacgtt aaatcagcag cgcactgata
      121 aacatccaga tctgattcat ggctaattgt tctgacattt tctacagaaa tacgcacatc
      181 cttatccagt ttcatccctt tctcgattgt ctttcttgca atatccccga ccagattttt
      241 tgcatcatca ctactacatt ttggcgtttt ctcgccgcag ccagccagca aaccagcagc
      301 aattaatacc agaagtattg ctcttttcat ctgtagttcc ttcgtaaatt aatgatactt
      361 ttatttctta ttaaatagac catccattgc gcctgccgca gaagccccgc agtatccctc
      421 gcaaccacgt gtcataatgc tggccttacc gtttgtcagt tcactaatta ctgcaacaca
      481 ctcgctggca ttgtcattcc agacccagcg acgcttctga ctttctccgg cagctatttg
      541 ctgtgctgtc ccctccatat tgcatacgcc cgaaccatcc cccgttgagg cattaatgtt
      601 gaattcagcg ttattctgtc ccggatgcag ggttagaacc gctccgggtt ttacgtactg
      661 cgctgctgcg actggtagcg ttatggctgc catgaataag gccaacagtt ttctgttcat
      721 taattaattc cctgttaagt gatacggatg tttttggtga ttttccccag tgccagcggt
      781 ctgcgttttt ccacaaagaa ataaacgacg ttgaatgcag acttgcgatg attccctcga
      841 ccaagagagg gccagttgcg gaaggattca atctgttctt ctgaataggg tgtgtcagag
      901 aaatcaaaca gcacaagcaa ggctttgccg tcatgtttca tcaatttctt tacttctgta
      961 ctgttgacgg tcgcagagac attggaacgt gccgcagttg gtttacgtac tgcaaactca
     1021 acggccacat cgtcaatgac aaaatcaatg taaccgtgcc cggatacagt tcctggcagt
     1081 ttgcttttta cttccggtga gacgttatct gcaaaccaac ccagcaaata gcaacgtacc
     1141 agaggcagta attcccgctc gctacattcg ttcagacgca gggttttgac gaactccctg
     1201 cgataaagag aactgaaaaa atactcaaag cattccgtta tttcagtgat ggtggttgcc
     1261 atggttgcat cctttacctg ctattaggtc catttttaag ggcatacctt tccggccata
     1321 atgtttccgg tgtcgtatcc agagccccgg caataatgcg ctcagctctg ggataatgac
     1381 gctctaaggc gttatttaat gtccgtggcg caaggccgtg actgcgtgac agtaacgcca
     1441 gcgttaaccc tcgcttgtga agtgctgcca caatatcggc acgatgccag ttccctgaac
     1501 ttcctttgat ttgctttttc tgcgatgctg ttgagttacg cacagtatta actccatgga
     1561 atcagtacat ggatattaaa tgcgaataaa agataccttt caagttcttt tggtatctat
     1621 ttattggttt tcatattaac gcattgatat ctggtgaaat ttatatgagc agtaagagaa
     1681 cagtttcggc tatacctact catcctgaag cttctgttga agaggctaaa gactggctga
     1741 cagcagagga attcactcat attgagggga tgccgggcac tgcgcgtggc gccaggatgc
     1801 gcttggaaaa gctggcatcc ctgcacccgg aaatacgtcg taaaaggact gccgggaaag
     1861 gtgtggttta ccatattagc gcagccacaa tgtcgcttga tgatgagaga aacagggcta
     1921 agggcgaagc gtttactctc aacgacagtg atcgccagct aagcctctgg gtgcagctgt
     1981 atcgcaatat gtcgccagct tcgcgggcgc aactgatgga gcaggccctc aggctggtaa
     2041 aggacgatct gatgtctcag gggcccaatg tttctgaact ggcggtacaa atcatgaagc
     2101 tgcccgaaga tgagcgtaag cgcctctttg aaaagctgaa cgggaaggtg tgatagcgtc
     2161 agttttccgc catgccagac agaaggaacc accccggaat cctgacctgt ctgacatggc
     2221 agaatggaaa gtatgccatc acttaataat gaataaaaac ctattgccag ttatttcatt
     2281 gctgaaccac a
//
LOCUS       DACADA010000317         2278 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.325, whole genome shotgun sequence.
ACCESSION   DACADA010000317 DACADA010000000
VERSION     DACADA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.325"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            223..561
                     /locus_tag="ISB17_004820"
                     /pseudo
     CDS             223..561
                     /locus_tag="ISB17_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            747..1961
                     /locus_tag="ISB17_004821"
     CDS             747..1961
                     /locus_tag="ISB17_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1982647.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            1975..>2278
                     /locus_tag="ISB17_004822"
     CDS             1975..>2278
                     /locus_tag="ISB17_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982648.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIK"
BASE COUNT          704 a          482 c          473 g          619 t
ORIGIN      
        1 cagacaacca atatcactta aataagtgat agtcctgata caaattttta gagtagtcat
       61 tagtgaacag ataattgtta acttagaacc agtaaaatgc aaacgacgca ccacgctggg
      121 aaaatcgctg ctattcagca gagttttgta ccgagaattg tggccagcca cttattgctc
      181 tcgcgtaagc aggtatcccc atcgtttacc agtccagcac atcttgtggt caggcagaag
      241 aagagcaaac tgttactgga ctcactgcat gcatggtgga gaaaaatgcc acgctatcca
      301 aaaatccaga ctgggtgaat ggttcagctg tgtactgaat cagtggggcg cactctgtta
      361 ttacagtgtt gacggtcagg cggaggcaga cataatgccg cggaaaaagc cctttgtgcc
      421 atctgtctcg gaaataaaaa ctttatgttc ttcggcggcg agcgcagtgc actgttgtat
      481 gggcttatcg gcacttgcct tctgaacgac attgatccgg aagcgtatct gcgccatatc
      541 ctgagcgtac tgccggaata accttccaac cgtattgatg aactcctgcc atggaacgta
      601 gttctcacca ataaataagc atcaatatga ttctccatta acgcttacct tttctcaggc
      661 tatttttgct tttccccgcc actttatggt gattatcaca tcaatataca tcgatagata
      721 ttaacgtaac tgaagtgagg tgaagcatga aatttgaacg ccatcatgaa atattgaaac
      781 gactcagtaa attcggttct gtaaaagtat ccgatctgtc caatagcctc aatgtaacga
      841 aagaaactat tcgatcagat cttaatgaac ttgccaggct tggctatctg actcgatgtc
      901 atggcggtgc ttttatcgtc ctagactcgc ttgatactat tgcgaaaaat gaaatcgctt
      961 atgcgcttga aaattatgat acagcccagg gaataaaaaa aggccactca actatgaaaa
     1021 gtcaagtatg cgttattggt tcgtttaatg ttgatatcat cagttatttg ccgaggcttc
     1081 ccactatcgg agaatcgctt ctggcaagta attttatatt ttcaccgggg ggaaaaggct
     1141 gtaaccaagc gctggcagca agctttgccg atactgatgt ttatttcata acaaaagtag
     1201 gtacagatca ctttagtgat tatgcgatca actttatgaa ttcgtctaag atttataaaa
     1261 gtataattta tcaaacgaaa gaaacgcaaa ccggaactgc cacgatcctt gttaatgaag
     1321 gtactggcga taatgttatt gcgatctatc ccggtgccaa catgacaatg tcgtccgatg
     1381 aaataactat tcagaaagaa gcgattatta attctgatgt catacttctg cagctcgaaa
     1441 caaattatac cgctctgcaa caagcgatca cattggcgca aaaaaacagt atcccggtca
     1501 ttattaaccc ggcaccctat aatgatatcg tcaacgaact tattcaggat gttgattata
     1561 tcacccccaa tgaaacagaa gctggtttac tgtccggcat agacgtacac gatcttgaat
     1621 cagctaaacg agctgcagaa gctatccaca ataaaggggt aaaaaacacg gttatcacgc
     1681 tcggtagcaa gggctcgctc gcatttgatg gcaaaaaatt cattcattcg ccagcgtttc
     1741 cagcggtcgt taaaaatacg gcaggagccg gggatgcttt taacggcgct ctggcatcag
     1801 gacttgcgaa aggaaaatca ctggaatcag cactgtgtta tgccagcgcc tttgcatccc
     1861 tagcggtgga gacaagcaat gcttccgaca tgcctgaaca tgaatctgtg atacatcgaa
     1921 ttcagagtat acattatcag caaactattt ttacgcatta agaagggaca gagaatgaat
     1981 aacaccaaaa aatcactaaa agtgctcttt attggggaat cctggcatat tcatatgatt
     2041 cattcaaaag gatatgacag ttttacctca agtaaatatg aagaaggtgc gacctggtta
     2101 ctccaatgtc tgaaaaatag tcaggtagat gtcacctata tgccggcaca tacggtacaa
     2161 attgccttcc ctgaagatgt ggcacagtta gaacaatatg atgccatcgt tattagtgat
     2221 attggtagta atactttcct gctacaaaat gatacttttt atcaacttcg cataaaac
//
LOCUS       DACADA010000318         2203 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.326, whole genome shotgun sequence.
ACCESSION   DACADA010000318 DACADA010000000
VERSION     DACADA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.326"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(359..1831)
                     /locus_tag="ISB17_004823"
                     /pseudo
     CDS             complement(359..1831)
                     /locus_tag="ISB17_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1983..>2203)
                     /locus_tag="ISB17_004824"
     CDS             complement(1983..>2203)
                     /locus_tag="ISB17_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP1982649.1"
                     /translation="SGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTES
                     VTIDLAYEGSGSGDWRTDAFIVGIGYRF"
BASE COUNT          383 a          639 c          607 g          574 t
ORIGIN      
        1 caacttttgt cagggttgcc agactggcaa catcattccc cgtaaaatac ggaaacctgt
       61 ctgccgcagt agcaagcccg gccagcgccg tcagggtggc atctttcggt tgcttacccg
      121 caagcgcgct agtcatggtg gtcgcaaaat tcgggtcgtt gcccaacgcc gcagccagct
      181 cgttcagcgt attcagtgcg tcaggcgacg agtccacgag ggcagcgatc gcagccataa
      241 cataagccgt gcttgcgatt tgggtattat tcgttccctg tcgcgcagtt ggtgttgttg
      301 gcgttccggt cagtgcagga ctatttaagg gcgctttctt gttcgtttca cccattaccg
      361 ccttaaccgc ttttggcgtt gccgccagtg actcggaagt gctgttggtc gcactgctga
      421 gctgtactat cccctttttc gtcgtgctcg catcctcaag cgccacggcg gatgcaatat
      481 cctctgcccg ttttgccgct gtctcggcgc gcgttgccgc ggattccgcc gtacttttgc
      541 tctgagctgc cgccgtcgca ctgccagctg cctctgtcgc cttcgtggat gccgtcgtgg
      601 cgctgccctt tgctgctgac gcctgtctgg tcgcctcatc ttttgaagca gacgcagatg
      661 atgccgatga cgccgctgaa ctggctgacg atgcggctgc cgccttagag gaagcagcat
      721 tgtctgctga agtctttgca tttgtttcag aggtttttgc tgcagaagca gacctcgctg
      781 ctgcagtggc ttgctcagtg gctttgccag ccttcgttgt ggctgttgaa gcggatgatg
      841 cggcgctttc tgccgatttt ccggcggcgg tggcactggc tgaggcctgc ccggcacttg
      901 ttgacgcggc actggcagat aatgcagccg ctgtttttga acctgccgca gctgaggcgc
      961 tctgtcccgc tgctgtttca gaagacttag cgttcgtctc ggacgttttt gccgccttcg
     1021 cggaatttcc tgccgccgtt gccgaggaag ctgcactact ggcacttgat gatgcattcg
     1081 tttctgaaga tttcgctgcc tcttttgagg ccgccgcacc ccgtgccgag gtggcagctt
     1141 ctgacgcctt cgtggtcgct gtggatgcag aagtggctgc cgatttttgt gatgctgcgg
     1201 cattcgtttc tgacgttttc gcggcactgg cgctggtagc tgccgcgctt tttgatgact
     1261 ctgcagcagc agcacttttt gctgcttcac gggcctttgt cgatgccgtt cctgcgctgg
     1321 aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggcg gcacgtgatg
     1381 aggctgcagc atcggttgca tgagttgccg cctcgctggc tgatgcactg gcatcgctgg
     1441 ctgatttttt cgcggctgcc gtattctgtg caaccgcgga ggcgttacgt gacacctctt
     1501 ctaccatctg ctcaaagcgg cgcagtgcct ccggtcgtac atcatcctcc gtcatggcac
     1561 cgagaaaatc attcagcgta cctggtctgg aaccttcata gacggtaatg gtcccggcat
     1621 gtgaaggcgg aaaaccttca accagcaggg tgacgctgta ctgaccatgc tcaacatcca
     1681 tgctgtaacg tccggcttca tccggatttt cagaggccac cgtgttcacc accaccgtgc
     1741 tgctggttcg tctggccttc agcacaatgg tgcagttctg tactggtttt cctgtgccat
     1801 ctttaagcac gccagaaatt tttactgtca tacttttcca ccaataaaaa aagcccgcag
     1861 cagtgacgcc acgggcttca ggacagtgta actttacgtt tcctcaaacg cagttcaccc
     1921 cataaggtgg atgaacctgc gtatcataac aatatttaca gaagataaat cggcgtctgt
     1981 tgtcagaaac ggtatccgat accaacaata aatgcatccg ttcgccagtc gccactaccg
     2041 gaaccttcat aagcaaggtc aatggtcacg gattcggtcg ggttaaactg cacgccagcc
     2101 ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc
     2161 gttttcccct tgttgtcagt tacgcggaga taatccccgg aga
//
LOCUS       DACADA010000319         2202 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.327, whole genome shotgun sequence.
ACCESSION   DACADA010000319 DACADA010000000
VERSION     DACADA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.327"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            46..675
                     /locus_tag="ISB17_004825"
     CDS             46..675
                     /locus_tag="ISB17_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAP1982650.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRSVTKLPLMGKSLHKT
                     IERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEKAITDFMTARGYH"
     gene            complement(724..>2202)
                     /gene="pitB"
                     /locus_tag="ISB17_004826"
     CDS             complement(724..>2202)
                     /gene="pitB"
                     /locus_tag="ISB17_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAP1982651.1"
                     /translation="LDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQ
                     LAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNL
                     GTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGL
                     VIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSH
                     GANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQK
                     LIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLR
                     RIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTM
                     IGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGT
                     MVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          584 a          578 c          542 g          498 t
ORIGIN      
        1 ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctc
       61 attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga
      121 aatgtcggtc gttcagtgac aaaattaccg ttgatgggaa aatccttaca taaaacaatt
      181 gaacgaaatc aggtgaaaac agcaaaaaaa ttgccaggac cagttccggc gctggtaatt
      241 acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc
      301 gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc ttacgatggt
      361 acagtgttcc cacctaacat tgaaggtggt cgttttgtct catggctggc aagccaggaa
      421 cgtaaagcgt ttcactggat ggcgagccat aaacctgatc tggtcatcaa actcaatgtt
      481 gaccttgaag ttgcctgcgc gcgtaaaccc gaccataaac gggaatcgct ggcgaggaag
      541 attgccataa cgccacagtt aacctttggc ggtgcacaac tggttgatat cgatgctaat
      601 cagccactgg aaaaggtgct gattgatgca gaaaaagcga ttacggattt tatgaccgcg
      661 cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga
      721 aggttaaatc aactgcaatg ctatccagta caacccacca gaaagaaaaa tcgccgccgg
      781 taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc
      841 gtccaccacc atcgttcctg ctacggcaga tgagagtacg tgtgttgtgg agacgggcat
      901 cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg ccatgccttg
      961 cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca
     1021 gccaatcatg gtgccaatgc cgagcgccag tgctactgcc atgatgatcc ataccggtgc
     1081 gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct ggtcttcttt
     1141 actgacgcct ggcagtttcg ccagcttcgc ggaagtatca gagatgcaca gcataatgcg
     1201 gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtag ctttccatat tgcctggcag
     1261 catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt gaaactccgt
     1321 cacttgcgcg ccatcagtcg atgccgcagg caatggaggt tccatcgcaa tcaacttctg
     1381 cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtgacggcat cgcgggtacg
     1441 ggtaatttca tagccggacg cgttcatatt gacgacgaag ccagcagggg caatccccac
     1501 cagtaccagc attaccaggc cgatcccttt ctgcccgtcg ttagcgccgt gcgaaaacgc
     1561 cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggct tacgtttgcc
     1621 ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct
     1681 ccagaaacgg cgcagcagga aaatcagacc acccgcaatg accaggccga cgatagggga
     1741 aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat ccatcaccga
     1801 tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg
     1861 cgaactggag gccggtaaac caaagaacca cgttcccagg ttccagataa tcgccgccag
     1921 cagcatggaa aagaccatcg ccaggccgtg agttgacccc atattcagca acaaatcggt
     1981 tggtaacata tggacaatgg cataggcaac gctaagtccg cccaataaca cgccaaaaaa
     2041 attaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag tataaataac
     2101 ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga acaacacaaa
     2161 tgccagagca agtaataaca aaagccctgt gtatatatca ag
//
LOCUS       DACADA010000320         2171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.328, whole genome shotgun sequence.
ACCESSION   DACADA010000320 DACADA010000000
VERSION     DACADA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.328"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            85..642
                     /locus_tag="ISB17_004827"
     CDS             85..642
                     /locus_tag="ISB17_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982652.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNAMIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGFELGWSDFYTT
                     TRKPSAGDVGAYTRTECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
     gene            645..1178
                     /locus_tag="ISB17_004828"
     CDS             645..1178
                     /locus_tag="ISB17_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1982653.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSADGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVV
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1207..1734)
                     /locus_tag="ISB17_004829"
     CDS             complement(1207..1734)
                     /locus_tag="ISB17_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1982654.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITANRRTDDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     NRVDTANPEWPQKPE"
     gene            complement(1736..>2171)
                     /locus_tag="ISB17_004830"
     CDS             complement(1736..>2171)
                     /locus_tag="ISB17_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982655.1"
                     /translation="LNGALGIGTSSALGGNSIVLGDNDTGFKQNGDGNLDVYANYVHV
                     MRFVPGSIQSNKTINITGRVNPSDYGNFDSRYVKDVRLGSQQYYGVNNWRTWNFQCPS
                     GHVLSGINVQDTGSNSADNIAGVYYRPVQKYINGTWYNVASV"
BASE COUNT          620 a          471 c          481 g          599 t
ORIGIN      
        1 aatcgacaaa cattcagcgg tggcctttct ggggaactgt ccggcaatgc cgctactgca
       61 acaaagctga aaacagcacg aaaaattgct ggagttggtt ttgatggttc tagcgatatt
      121 tcaattagtg ccaaaaatgt caatgcattt gcactccgac aaacaggtaa tactgttaat
      181 ggtgatacat ccgttggatg gaattgggat agtggtgcat ataacgccat gattggtggt
      241 gcatctgcat taattcttca ctttaatata aatgctggta gctgtcctgc cgtacaattc
      301 cgtgtgaatt ataaaaatgg tggcatttcc tacaggtcgg ctcgtgatgg ttatgggttt
      361 gaattaggtt ggtcagattt ctataccacg acacgaaaac cttcagcggg agatgttggt
      421 gcatatacgc ggacagaatg taactcaagg tttattacag gtattcgcct tggcggtctg
      481 tcatctgttc agacatggaa tggtcccggc tggtctgaca ggtcaggtta tgtcgttacg
      541 ggttcagtta acggaaaccg tgatgaatta attgatacaa cacaggcaag gccaattcag
      601 tattgcatta atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa
      661 acattactgc tggcaaccct aaaacaaaag agcaatacca gctaacaaag caatttaaca
      721 tcaaatggct ttattcagct gatggaaaaa actggtatga ggaacaaaag aatttccagc
      781 cagacacttt gaaaatggtc tatgaccata acggcgttat tatttgtatt gaaaaggatg
      841 tttcagcaat taatccggaa ggcgcaagcg tcgttgaatt acctgatatt acagcaaatc
      901 gccgggctga tatttcgggg aaatggatgt tcaaagatgg cgtagtggta aagcgaactt
      961 ataccgagga agagcagagg caacaagcgg aaaatgaaaa gcagagcctg ttgcaacttg
     1021 tcagggataa aactcagcta tgggactcac agctacggct gggcatcatt tccgacgaga
     1081 ataaacaaaa attaaccgag tggatgctct atgcgcagaa agtcgaatcc acagacacct
     1141 ccagcctgcc agtaacattt cccgaacaac ctgaatgaga caaggcccga taacgggcct
     1201 taatttttat tcaggctttt gtggccattc aggatttgcc gtatccacac ggttgaccag
     1261 aacactgtag cgttcccatg cttccagtcg tgtgcgttcc tcgtctgttg ccatattcag
     1321 cctgacagcg cgttccagcg gctggatgac tgattcagct tcggaaagca atgcggcctt
     1381 ttgtgattcg gcctgttgtt gctgttcgtc agccgtataa atccgtttaa ccacagctcc
     1441 gtccttaaac atccacttac cggaatcatc ggtccgacga ttggccgtta tatcgggaac
     1501 ctcgacgacg ctaaaacctt cgggattaag cgtggaggca tctctggtga tggcgacaat
     1561 aatattattt tcatcgtaaa caatctttat ggtgtctggc tgaaagtttt tcacttcctc
     1621 ataccagttt tttccgtcct cagagtaaag ccagataact ccgtgtttct ttgttaactc
     1681 atactgttcc agtgttttag cattacccgc ttttatgttc tttaagtgca tcatattaaa
     1741 cgctcgctac attataccag gtgccattta tatacttttg aacgggtctg taataaacac
     1801 ccgctatatt atcggcagag ttagaccctg tatcctgaac attaatacca gacaatacat
     1861 gacctgaagg gcactggaaa ttccatgttc gccagttgtt cactccataa tattgctgtg
     1921 aaccaagtcg aacatctttc acataacggg aatcaaagtt accgtaatcc gagggattaa
     1981 cacgccccgt aatatttatg gtcttattac tttgaatgct tccgggaaca aagcgcataa
     2041 catggacgta attagcataa acatccagat taccatcgcc attttgttta aagcccgtgt
     2101 cattatcacc caatacaatc gaattaccgc caagagcact ggatgttccg atacccagtg
     2161 caccattcag t
//
LOCUS       DACADA010000321         2123 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.329, whole genome shotgun sequence.
ACCESSION   DACADA010000321 DACADA010000000
VERSION     DACADA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.329"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            158..1699
                     /locus_tag="ISB17_004831"
     CDS             158..1699
                     /locus_tag="ISB17_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HAP1982656.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1714..>2123
                     /locus_tag="ISB17_004832"
     CDS             1714..>2123
                     /locus_tag="ISB17_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1982657.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKAL"
BASE COUNT          599 a          509 c          501 g          514 t
ORIGIN      
        1 ccatcgagat cgggtaataa catttgaaca gatcgctgaa taacatcgat ggagatcact
       61 tttgactcat tttgttattc agtgatctcc atcaatgtta ttggaacttc acaggtgtgt
      121 tgatctgtat cttttgccat tccggtaaag gatacctatg ccaacagttc caatttctat
      181 gagaaaactt aaagaaattc ttaggcttaa atacggtgtt ggactcagcc atcgacaaat
      241 tggtcgtagt cttgcaatct ccccttccgt tgtatccaga tatgctaatc gggcggctca
      301 acttggcata aagcagtggc ccttacctac aggatgggat gatacaaaac taaaacatgc
      361 gttccttcag acccaggtta agatgaagaa gcactctctg cctgactggg ctacagtaca
      421 ccgggaactg cgtaataaat gcgtgacgct gcagctactc tgggaagaat actgtgagcg
      481 taatccaggc ggtttttaca gctataacca ttactgccgg atgtaccgtg aatggctcaa
      541 aaccacttca ccatcaatgc gtcaggtaca taaagctggc gaaaaacttt tcgttgatta
      601 ctgtggacct accgttggcg ttaccgaccc tgagaccgga gaaataagaa ctgctcaggt
      661 catcgtagct gttctcgggg catcaagtta cacatgggca gaggccacct ggtctcagca
      721 gcttgaagac tgggtgatga gtcatgttcg ctgcttccag tggttgggtg gcgttcctga
      781 acttgttgtt ccggacaatc tgaaaagcgc cacatccagg gcatgtaagt atgatcctga
      841 cgttaaccct acctaccagc agatgcttga gcattataat gtcgcagttt tgcctgcgcg
      901 gccacgtaaa ccgaaagata aagccaaagc tgaagttggc gttcaggttg ttgaacgctg
      961 gatcatggcc cgaatcaggc atgagatctt ctacagcctt gcatcgctta atcagcgcat
     1021 tcgggagttg ctggaaagac tgaataacaa aataatgcag aagttgggtt attcacgtgc
     1081 agaactcttc atccagcttg ataaacccgc actgaagcct cttcctgaag ccagttacag
     1141 ttacaccctg gtgaagaaag tcagagttca tgccgattac cacgtggaaa tcgacaaaca
     1201 ttactactcg gttccatgtt cgctgttagg ccagcaactg gaagcatgga tctccggaga
     1261 actggtaaga ctcttcaatc aggggcagga ggttgctgtg cacccgcgca agcgtactta
     1321 tggctacagt acccgcaacg agcacatgcc tgaagctcat cgacagcatg ccacctggac
     1381 gccagagcgt cttctggaat gggcggggca cataggcagt gaaactcata gttatgtgct
     1441 tcatatactg aactctcgtc cacatccgga acaaagctat cgcttctgcc ttggactcct
     1501 gaaccttcat aaaaaataca gtaaagccag acttaatgca gcatgtgcaa gagctctgaa
     1561 aacaaaggta tggcgtctgt caggtattaa atcgatcctg gaaaaaggtc tggataaaca
     1621 acctgttcag gatccaaaac cagatctgtt atccacgatg gaacacgaaa acgtacgcgg
     1681 cagtgagtat taccactgat acgggatcca atgatgaatc atctttacga acaactgacc
     1741 gcacttaaac tcaccggctt ccgtgatgcg cttaaaaagc aacttgctca accgggcaca
     1801 taccaggagc tgggcttcga agaacgcctg tcattactga cagcagaaga actaacctgc
     1861 cgtgaaaaca ggaaggcaga gcgtctgatc aaacatgcac ggttcagact taatgctgag
     1921 ttatcaaagc tggattatcg taacaataga gggctggaca gggccctcat ccgttcactc
     1981 agtcagggaa actggttaac cctgaaacaa aatattttac tgaccggggc caccggcagc
     2041 ggtaaaacgt tcctggcatg tgcacttggt cataatgcct gccgacaggg atacaaggtc
     2101 tactattatc gccttaaagc gct
//
LOCUS       DACADA010000322         2090 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.330, whole genome shotgun sequence.
ACCESSION   DACADA010000322 DACADA010000000
VERSION     DACADA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.330"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..411
                     /locus_tag="ISB17_004833"
     CDS             <1..411
                     /locus_tag="ISB17_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1982658.1"
                     /translation="VDVRIFPESQIVNFGQISANSIATYRPKAAFSVSTIKDVAADCT
                     EQFDVVTSFYTTDTLHDDTHLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGA
                     MVTRDYQAELSQKPGETLVYGPFKKDLIVKINYN"
     gene            complement(465..1118)
                     /gene="ribB"
                     /locus_tag="ISB17_004834"
     CDS             complement(465..1118)
                     /gene="ribB"
                     /locus_tag="ISB17_004834"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAP1982659.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(1222..1370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1492..1791
                     /locus_tag="ISB17_004835"
     CDS             1492..1791
                     /locus_tag="ISB17_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HAP1982660.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPAAELQSPPAIPPVDKAE"
     gene            complement(1826..2026)
                     /gene="glgS"
                     /locus_tag="ISB17_004836"
     CDS             complement(1826..2026)
                     /gene="glgS"
                     /locus_tag="ISB17_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAP1982661.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT          582 a          534 c          462 g          512 t
ORIGIN      
        1 gtcgatgtcc gtatttttcc agaaagccag atcgttaatt tcgggcaaat ctctgcaaac
       61 tctattgcta cttatcgacc caaagctgca tttagcgtat ctactataaa agatgttgct
      121 gccgactgta ccgagcaatt tgatgttgtc accagttttt atacgacaga tacgttacat
      181 gatgatacgc atctggaaat gggtaatggt ttgttaatgc gtattactga tcaaaaaaca
      241 caagaagaca ttaagtttaa taagtacaaa cgcttcacca catatattcc aggtcagact
      301 ggagcgatgg ttacccgtga ttaccaggct gaactttcac aaaaaccggg ggagactctc
      361 gtttatgggc ctttcaaaaa agatctcatc gtaaaaataa actataactg aacagcaaaa
      421 tgccgggaac accccggcat tttcatattt agtatttcac gtcattagct ggctttacgc
      481 tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat attgtgttta
      541 ttcgcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca
      601 cacagcacac cagctggttt aaagcctgcc agcgtcatca gatcgatagt cgcttcagta
      661 tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac gtggcctggg
      721 cgattcagat ctgaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacgg
      781 tcagcggcag aaacaccggt agtcacacct tcagctgctt caatggtcac ggtaaaaccg
      841 gtgccatagg cgctggtgtt attttctacc atcattggca gatcgagttg tttacggcga
      901 tcgtcagtaa tgcacaggca aacaatacca ctcccgtggc gaatggtcag cgccatctgc
      961 tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg gtcttcatca
     1021 tcaagcacca tcacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg
     1081 aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat
     1141 tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa ataacgtgag
     1201 cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc gtcggccccg
     1261 gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac
     1321 tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc ggattcacta
     1381 taacgctaat gattagcgcc agcaacgcat agcttcacat aattctggtt tatgacttac
     1441 ccttatcgca ctacaatggc actcaacacc tatcagtaca gggaaactac aatgattgac
     1501 ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa aggaatcagg
     1561 gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagcgca gctgacgcgt
     1621 ctcgatctgg taagccgcga agaattcgac gttcagacgc aagtcctgtt acggactcgt
     1681 gaaaaactgg cgttactgga gcagcgcatc agcgaactgg aagcgcgcaa taatccggca
     1741 gcagaacttc aatctcctcc ggctatcccg ccagttgata aagcagaata aagcaccaca
     1801 aaaacgggcc agacggcccg taatatcagt gttctaattc aagttctctg gtttgcatca
     1861 tctgttggca ataccaggca taacgtgcgc aaaaccaggt tctatgttct tcatccatgt
     1921 taccagtaac ggccagaata tcgaccgggc gaccttctgc gtgcattctg gcaaaactac
     1981 gcgcgagaaa atcgaaatta tttaaagaat taagactatg atccataagc acatcctccg
     2041 gttattgcat accttttaag gccatatgct ttttctgatt tcagtattcc
//
LOCUS       DACADA010000323         2085 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.331, whole genome shotgun sequence.
ACCESSION   DACADA010000323 DACADA010000000
VERSION     DACADA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.331"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            87..1220
                     /gene="ompN"
                     /locus_tag="ISB17_004837"
     CDS             87..1220
                     /gene="ompN"
                     /locus_tag="ISB17_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAP1982662.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1361..1795
                     /gene="uspF"
                     /locus_tag="ISB17_004838"
     CDS             1361..1795
                     /gene="uspF"
                     /locus_tag="ISB17_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAP1982663.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1884..>2085
                     /locus_tag="ISB17_004839"
     CDS             1884..>2085
                     /locus_tag="ISB17_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP1982664.1"
                     /translation="MNYALIIAIIISFPCALLSVFLVLKGWALIGDAMSHAMFPGIVL
                     VWIIGMPLGIGAFIVGLSCAIAT"
BASE COUNT          557 a          467 c          477 g          584 t
ORIGIN      
        1 tcggctaact gaacttctcc tttaggatgt tttcactccc cgaaatgggg atgaaaggca
       61 aataaaataa ctaaaggatt tattcaatga aaagcaaagt actggcactc ttaattcctg
      121 ccctgctcgc tgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat
      181 tggatctgta tggcaaagtt gatggcctgc attatttttc tgataattca gcgaaagatg
      241 gcgaccagag ctatgctcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactca
      301 ctggctacgg tcaatgggaa tacaatattc aggcaaacaa caccgaatct tcaacaaacc
      361 agtcatggac ccgtctggca tttgccggac tgaaatttgc agattacggt tctttcgatt
      421 acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gactgatatg ctacctgaat
      481 ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgtgcc aatggcgtcg
      541 cgacttatcg taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt
      601 atcaaggtaa caacgaaggt gccagtaatg gtcaggaagg caccaacaac ggacgtgatg
      661 ttcgccatga aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct
      721 ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgaccaggtt aaccatactg
      781 cggcgggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata
      841 tttacctggc aaccatgtat tcagaaacgc gtaatatgac cccgtttggc gacagcgatt
      901 atgctgttgc aaacaaaacc cagaactttg aagtcactgc acagtaccag tttgattttg
      961 gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg tgacctgcac gctgccggtg
     1021 gtgcagacaa tccggcaggt gttgatgata aagatctggt taaatacgcc gatgttggcg
     1081 cgacttacta tttcaataaa aacatgtcca cctatgttga ctataaaatc aacctgttgg
     1141 atgaagatga cagtttctac gctgccaacg gcatctctac cgatgatatt gtcgctttag
     1201 gtctggttta tcagttctaa atccccctgc ccgctgtttt ggcgggcttt ttctgcttat
     1261 tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa aagtgtcatc
     1321 tggcgttaca ctttatgcgg atactaaaac aggaggtttt atgaacagaa cgattcttgt
     1381 ccctatcgat atttccgatt cagaattaac tcaacgcgtg attagccacg ttgaggcaga
     1441 ggcaaagatt gatgatgcag aggttcattt cctgacggta ataccttcgc tgccctacta
     1501 tgcctctctg ggtttagcgt attccgcaga attaccggca atggatgacc tgaaagcgga
     1561 agccaaatcg caactggaag agatcattaa aaaatttaaa ctgccaaccg acagagtgca
     1621 tgtccatgtt gaggaaggct cgcccaaaga ccgcattctg gaattggcga agaagatccc
     1681 cgctcatatg atcatcattg cttcccatcg accggatatc accacttatc tgctcggttc
     1741 taacgccgca gctgtagtgc gtcacgcaga gtgctccgtg ctggttgtgc gctgacacta
     1801 acgcccgcac attgctgcgg gctttttgat tcatttcgca aatgtgctga tattttcccc
     1861 tctaatcatt acaatttaaa tttatgaatt atgcattgat aatcgctatt ataatatcgt
     1921 tcccctgtgc attattatca gtttttctgg tattaaaagg ctgggcgcta ataggagatg
     1981 caatgagcca cgcaatgttt cctggcatag ttctagtctg gattataggt atgccgctgg
     2041 gaattggtgc atttattgta ggactgtctt gtgcaattgc aactg
//
LOCUS       DACADA010000324         2084 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.332, whole genome shotgun sequence.
ACCESSION   DACADA010000324 DACADA010000000
VERSION     DACADA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.332"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            108..701
                     /locus_tag="ISB17_004840"
     CDS             108..701
                     /locus_tag="ISB17_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982665.1"
                     /translation="MTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFD
                     SDMTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYG
                     QTNGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIA
                     WKFYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            722..>2084
                     /locus_tag="ISB17_004841"
     CDS             722..>2084
                     /locus_tag="ISB17_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP1982666.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETL"
BASE COUNT          592 a          406 c          510 g          576 t
ORIGIN      
        1 ctgcatattc ttcattagaa tataaaggaa cctttggttc aataaatgcg ggttatgcag
       61 actggaatag tggatttgta aacactcacc gtggtgaagt atggaaagtg actgcggatt
      121 ttggggtaaa ttttaaagaa gcagaatttt actcatttta tgaaagtaat gtactcaatc
      181 atgctgtagc agggcgaaat catacggttt cagtaatgac gcatgtcaga ctctttgact
      241 ctgatatgac attctttggc aaaatttatg gccaatggga taactcatgg ggtgacgatc
      301 tggacatgtt ttatggattc ggttacctcg gctggaacgg cgagtggggc ttttttaaac
      361 cgtatattgg attacataat caatctggtg actacgtatc agctaaatat ggtcaaacga
      421 atggttggaa tggttatgtt gttggctgga cagcagtatt accatttacg ttatttgacg
      481 aaaaatttgt tttatctaac tggaatgaaa tagaactgga caggaacgat gcttacacgg
      541 agcagcaatt tggccggaac gggttaaatg gcggtttaac tattgcctgg aagttctatc
      601 ctcgctggaa agccagtgtg acgtggcgtt atttcgataa taagctgggc tacgatggct
      661 ttggcgatca aatgatttat atgcttggtt atgatttcta attttttatc agggaataat
      721 tatgcagaaa acgttaatgg ccagtttgat cggccttgca gtttgcacag ggaatgcttt
      781 tagtcctgcc ttagccgcag aggctaaaca acctaattta gtcattatta tggcggatga
      841 tttaggttat ggcgatttag caacatatgg tcatcagatc gttaaaacac ctaatatcga
      901 caggcttgcc caggaagggg tcaaatttac tgactactat gcccccgctc ctttaagttc
      961 accttcacgc gcagggctat taaccggccg gatgccattt cgtactggaa ttcgctcatg
     1021 gattccttca ggcaaagatg ttgccttagg gcgtaacgaa ctcacgattg ctaatctact
     1081 caaagcgcaa gggtacgaca cggcaatgat gggtaagctg catctgaatg caggcagcga
     1141 tcgcaccgat cagccacaag cacaagatat gggctttgat tactcactgg ttaatacggc
     1201 gggctttgtt accgacgcca cgctggataa cgctaaagaa cgcccgcgtt atggcatggt
     1261 ttacccgaca ggctggctac gtaacgggca acccactcca cgagccgata aaatgagcgg
     1321 tgagtatgtc agttcggaag tcgtcaactg gctggataac aaaaaggaca gcaagccttt
     1381 cttcctctat gttgctttta ccgaagtgca tagccccctg gcttcgccca aaaaatacct
     1441 cgatatgtac tcacaatata tgagcgcgta tcagaagcag catcctgatt tattttatgg
     1501 cgactgggca gacaaaccct ggcgtggtgt gggggaatat tatgccaata tcagctatct
     1561 ggatgcacag gttggaaaag tgctggataa aatcaaagct gtgggtgaag aagataacac
     1621 aatcgttatt tttaccagtg ataacggtcc ggtaacgcgt gaagcgcgca aagtgtatga
     1681 gctgaatttg gcaggggaaa cggatggatt acgcggtcgc aaggataacc tttgggaagg
     1741 cggaattcgt gttccagcca ttattaaata tggtaaacat ctaccacagg gaatggtttc
     1801 agatacaccc gtttatggtc tggactggat gcctacttta gcgaaaatga tgaacttcaa
     1861 attacctaca gaccgtactt tcgatggtga atcgctggtt cctgttcttg agcaaaaagc
     1921 attgaaacgc gaaaagccat taattttcgg gattgatatg ccattccagg atgatccaac
     1981 cgatgaatgg gcgatccgtg atggtgactg gaagatgatt atcgatcgca ataataaacc
     2041 gaaatatctc tacaatctga aatctgatcg ttatgaaaca ctta
//
LOCUS       DACADA010000325         2070 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.333, whole genome shotgun sequence.
ACCESSION   DACADA010000325 DACADA010000000
VERSION     DACADA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.333"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1769
                     /gene="sat"
                     /locus_tag="ISB17_004842"
     CDS             <1..1769
                     /gene="sat"
                     /locus_tag="ISB17_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAP1982667.1"
                     /translation="HNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTK
                     GIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDA
                     ATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQS
                     SINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQLVVNKSLSGKN
                     NLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTPVIEQKNDTDKA
                     TWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDINGESGAWARIM
                     SGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGSDAFSGETKSVG
                     AGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYAGAEVGYRYHVT
                     DSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWK
                     VTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLEF
                     EKSAFGKYNVDNAINANFRYSF"
BASE COUNT          592 a          470 c          492 g          516 t
ORIGIN      
        1 cacataacag ccaaatagcc ataggtgatc aagctgtagt tacacttaat ggtgcaacct
       61 ttctgaataa tactcctata agtatagata aaggagcaaa agttatagca caaaattcca
      121 tgttcacaac aaaaggtatt gatatctccg gtgagctgac tatgatggga atccctgaac
      181 agaatagtaa aactgtaacg ccgggtctcc actacgctgc tgatggattc aggctgagtg
      241 gtggaaatgc aaatttcatt gccagaaata tggcatctgt caccggaaat atttatgctg
      301 atgatgcagc aaccattact ctgggacagc ctgaaactga aacaccgact atatcgtctg
      361 cttatcaggc atgggcagag actcttttgt atggctttga taccgcctat cgaggcgcaa
      421 taacagcccc caaagctaca gttagcatga ataatgcgat ctggcatcta aatagccagt
      481 catcaattaa tcgtctagaa acaaaagaca gtatggtgcg ttttactggt gataatggga
      541 agtttacaac ccttacagtg gacaacctta ctatagatga cagtgcattt gtgctgcgtg
      601 caaatctggc ccaagcagat cagcttgttg tcaataaatc gttgtctggt aaaaacaacc
      661 ttctgttagt cgacttcatt gagaaaaatg gaaacagcaa cggactgaat atcgatctgg
      721 tcagcgcacc aaaaggaact gcagtagatg tctttaaagc tacgactcgg agtattggct
      781 tcagtgatgt aacaccggtt atcgagcaaa agaacgatac agacaaagca acatggactc
      841 tgatcggcta taaatctgtg gccaacgccg atgcggctaa aaaggcaaca ttactgatgt
      901 caggcggcta taaagccttc cttgctgagg tcaacaacct taacaaacgt atgggtgatc
      961 tgcgtgacat taacggtgag tccggtgcat gggcccgaat catgagcgga accgggtctg
     1021 ccggcggtgg attcagtgac aactacaccc acgttcaggt cggtgcggat aacaaacatg
     1081 aactcgatgg ccttgacctc ttcaccgggg tgaccatgac ctataccgac agccatgcag
     1141 gcagtgatgc cttcagtggt gaaacgaagt ctgtgggtgc cggtctctat gcctctgcca
     1201 tgtttgagtc cggagcatat atcgacctca tcggtaagta cgttcaccat gacaacgagt
     1261 ataccgcaac tttcgccggc cttggcacca gagactacag ctcccactcc tggtatgccg
     1321 gtgcggaagt cggttaccgt taccatgtaa ctgactctgc atggattgag ccgcaggcgg
     1381 aacttgttta cggtgctgta tccgggaaac agttctcctg gaaggaccag ggaatgaacc
     1441 tcaccatgaa ggataaggac tttaatccgc tgattgggcg taccggtgtt gatgtgggta
     1501 aatccttctc gggtaaggac tggaaagtca cagcccgcgc cggccttggc taccagtttg
     1561 acctgtttgc caacggtgaa accgtactgc gtgatgcgtc cggtgagaaa cgtatcaaag
     1621 gtgaaaaaga cggtcgtatg ctcatgaatg ttggtctcaa cgctgaaatt cgcgataatc
     1681 ttcgcttcgg tcttgagttt gagaaatcgg catttggtaa atacaacgtg gataacgcga
     1741 tcaacgccaa cttccgttac tctttctgat aacagcccgg gccgcgtttg cggcccttct
     1801 tctaccggag agaatatgta ttaccctgtg acagactata tcgctcttgc tctcattatt
     1861 agctttcttt ttctgacatt atttatctgc ctgttatgcc ttaaacatga gcgaataaaa
     1921 aaagaaacta tcaggcaaaa aaatgcacat attctggatc atggctggaa tgcaactgag
     1981 gtagcctgag tttaacggac actccttcct gaaatagaat ggcatcagaa ggagctaata
     2041 atgagcagaa aaacccaacg ttactctaaa
//
LOCUS       DACADA010000326         2042 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.334, whole genome shotgun sequence.
ACCESSION   DACADA010000326 DACADA010000000
VERSION     DACADA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.334"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            40..2025
                     /gene="yjcS"
                     /locus_tag="ISB17_004843"
     CDS             40..2025
                     /gene="yjcS"
                     /locus_tag="ISB17_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAP1982668.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          537 a          536 c          519 g          450 t
ORIGIN      
        1 attacatatt tacgacataa atgataaatg gagatttata tgaataactc tcggttattc
       61 cgtttgagca ggattgttat tgcgttcact gccgccagcg gcatgatgat aaataccgct
      121 tacgcaacag atgaggcgaa agccgccact caatataccc aacaggttaa tcagaattac
      181 gccaaatcat taccgtttag cgatcgccag gattttgacg atgctcagcg tggatttatc
      241 gctccgctgc tggatgaagg tattctgcgt gatgcgaacg gtaaaattta ctaccgcgcg
      301 aatgattaca aatttgatat taatgccgct gcgccggaaa ccgtaaaccc cagcctgtgg
      361 cgtcagtcgc agatcaacgg catttcaggc ctgttcaaag tcaccgataa aatgtatcag
      421 gtgcgcggcc aggatatctc taacattacg ttcgttgagg gcgagaaagg cattattgtt
      481 attgacccac tggtgacgcc acctgccgca aaagccgcac ttgaccttta cttccagcat
      541 cgtccgcaaa aaccgattgt tgccgttatc tacactcaca gccacgccga ccactacggc
      601 ggcgtgaaag gtattatctc tgaagccgat gttaaatccg gcaaagttca ggtgattgct
      661 ccagctggct ttatggacga agccatcagc gaaaacgtgc tggcgggtaa catcatgagc
      721 cgccgtgcgc tctactctta cggtctgtta ctgccgcaca acgcgcaagg caacgtgggc
      781 aacggcctcg gcgtgacgct ggcaacgggc gacccgagca ttattgcacc gactaaaact
      841 atcgtcagaa ctggcgagaa gatgattatc gacggcctgg agtttgactt cctgatgacc
      901 ccaggtagcg aagcgccagc ggaaatgcac ttctatattc cggccctgaa agccctgtgt
      961 accgccgaga atgccacgca taccctgcac aacttctaca ccctgcgcgg cgcgaaaacc
     1021 cgcgacacca gcaagtggac cgagtatctg aacgaaacat tggatatgtg gggtaacgac
     1081 gcggaagtac tgtttatgcc gcacacctgg ccggtctggg gcaataagca tatcaatgat
     1141 tatattggta aatatcgcga tactatcaag tacattcacg accagaccct gcacctggcg
     1201 aaccagggct acaccatgaa tgaaatcggc gacatgatta agctgccgcc tgcgcttgcc
     1261 aataactggg ccagccgcgg ctattacggt tctgtcagcc acaacgcccg cgcggtatat
     1321 aacttctatc ttggctatta cgacggtaac ccggctaacc tgcatccgta tggtcaggtg
     1381 gagatgggta aacgttacgt gcaggcgctg ggcggttctg cccgcgtcat caacctggcg
     1441 caagaagcga acaagcaagg tgattatcgc tggtcggcag aactgctgaa acaggtgatt
     1501 gccgccaacc cgggtgacca ggtcgcgaag aatctgcaag cgaataactt tgaacagctg
     1561 ggctatcagg ccgagtccgc cacatggcgc ggtttctacc tgaccggcgc gaaagagctg
     1621 cgcgaagggg tgcataagtt cagccacggc accaccggtt ccccggacac cattcgtggg
     1681 atgtcggtcg aaatgctgtt cgactttatg tccgttcgcc tcgatagcgc gaaagccgcg
     1741 ggtaaaaata tcagcctgaa cttcaatatg aacaacggcg ataacctcaa cctgacgctg
     1801 aacgatagcg tgcttaacta ccgtaaaaca ctgcaaccgc aagctgacgc ctctttctac
     1861 atcagccgtg aagatctgca cgccgtgctg accggacagg caaaaatggc tgatctggtg
     1921 aaagcgaaga aagccaaaat tattggcaat ggtgcgaaac tggaagaaat tatcgcctgt
     1981 ctggataatt tcgatttgtg ggtgaatatc gtaaccccaa attaatatca ctcgcttttg
     2041 gg
//
LOCUS       DACADA010000327         1968 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.335, whole genome shotgun sequence.
ACCESSION   DACADA010000327 DACADA010000000
VERSION     DACADA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.335"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..856)
                     /locus_tag="ISB17_004844"
     CDS             complement(<1..856)
                     /locus_tag="ISB17_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982669.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSR"
     gene            1013..1933
                     /locus_tag="ISB17_004845"
     CDS             1013..1933
                     /locus_tag="ISB17_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1982670.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          569 a          439 c          426 g          534 t
ORIGIN      
        1 cccgagatga aacatatata taaaatccag gaaatgaaac ggcccaatcc tcaagaatcg
       61 gctgtaactt gcccgcattg atatgttcag cgattgcaaa atccagatgt tgaatgatac
      121 ccgttccatt cagcgccatt cgtatcatgc tttcgtcatc attagaaata tacttacctg
      181 acacctctat ttcgaactct tcattacccc ccacaacatt cgaaaactcc catgctgaga
      241 gtgcaccgct acttttatgc cgcaaccgaa gacaattatg gttactcaat tcctgaggag
      301 tttcaggaat accgtgttct tttaaataat ctggcgatgc cacagtaacc agtttaataa
      361 gcggagaaac aggaatggca gtcatatgct catccagtcc ttgctcaaga cgtatgccca
      421 catcacagcc gtccgcaata atgttagata aaccgtcatc cataatgagt tctatctttg
      481 ttttggggta acgagtaaga aactcgccaa tatgaggctc aacaagcatc cttgctgcaa
      541 cacgggagga atttattctc aacagtcctg taggttcaag ttgggtatca ccgacatctt
      601 tcaacgcatc atcaatttgc cccaaagccg acaccagcac ttcatacaga tgctgtcctt
      661 cctcagtaag cgacatattg cgggttgtac ggtatatcag cttggtgtta agacgttcct
      721 caagagattt taaattctgg gacaatgcgg cccgcgtaat acccatttca gaggctgctc
      781 tggaaaaact gccggcacga acaatcagcg caaaccaggc aagggaaggc aaaattgaag
      841 gctctatttt catcattgta aaccttaact taacactgtg atagatatta gaccattgat
      901 gataaccaat acaagtcata ctattacgcc gttcgcgata tgaacacgaa tgccagttgc
      961 ggataataga aatgatattt aaatctgttt aggcaaatcc ctggagcttg aaatgaaaac
     1021 tgtttcaata aaacacactt actgggatat agctgctgat atctatttcc ctgacgattt
     1081 taatagtgaa aaaaagtatc ctgccattat cagtgcccat ccaattggaa gctgcaaaga
     1141 acagacatcg ggtagtgtgt atggtgccgc cctggcaaaa gctggcttta tcgtcattgc
     1201 ttttaatgcc agttttcagg gaagcagcgg tggagaaccg cgttatctgg aagatccaac
     1261 aatgcgagta aaagacttca gcattgtcgt tgattacctt accaccttac cttacgttga
     1321 tgccgggcgt attggtgtac tgggtatttg tggtggaggt ggatatgcca ttaatgccgc
     1381 aatgacagaa cgtcggatta aagccattgg tactgtcaca ggtgcaaact acgggcgtct
     1441 tatgcgtgag ggatttacag cgtttaatcc tatcggagca ctcgaagcaa tggcgcaaca
     1501 aagaacagac gaagctaacg gcgcaaaact tcgcgtagat gatttgcttc cttcttctcc
     1561 ggaagcagct cttgaagcgg gattaacaga aatagacctt tttgaagcaa cagaatatta
     1621 tcgttcgcca cgaggctgtg cgcctaatgg ggtaaatcgt tcgctatttt cacatcagac
     1681 tgttgccgtg ggctgggatg cttttcatct tgcagaagtc ctgttaactc aaccactaat
     1741 ggttgtagta ggcgatagag tgggcgcttt tggtgcctat cgtgacggct gcgaaattat
     1801 tggtcgtgca gcctcaaaac ataaagagtt ggtcgtggtt gaaggttatt cacattacga
     1861 cctgtatgat aagccagaac ctgtgaaaca ggcgctcgat aaattaattc ctttttacaa
     1921 aacacatttg taataacctt gcagggagat tcattatctc cctgacat
//
LOCUS       DACADA010000328         1967 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.336, whole genome shotgun sequence.
ACCESSION   DACADA010000328 DACADA010000000
VERSION     DACADA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.336"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(77..445)
                     /locus_tag="ISB17_004846"
     CDS             complement(77..445)
                     /locus_tag="ISB17_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1982671.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(608..829)
                     /locus_tag="ISB17_004847"
     CDS             complement(608..829)
                     /locus_tag="ISB17_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1982672.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(892..1368)
                     /locus_tag="ISB17_004848"
     CDS             complement(892..1368)
                     /locus_tag="ISB17_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1982673.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            complement(1384..1857)
                     /locus_tag="ISB17_004849"
     CDS             complement(1384..1857)
                     /locus_tag="ISB17_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1982674.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          458 a          497 c          578 g          434 t
ORIGIN      
        1 ctgatatgca gagataaaaa atgggacagt ggcgtggtga aaacaccagg ccggagcaga
       61 aggttattct ggtggtttac tttttcgttt caggtgtcgg ataaacagcc agataaacgt
      121 aaccacagga gccgagggtg tcggcttcac aggtcagccc cttcgcatac agtgtcacgg
      181 tatgctgatg gcggggattc agttcaccac tggtgagcat taatttcagt tgtttcatca
      241 gcagcggaaa ggcctggtcc agagggtacg catccgcatc tctgaaccgg cctctgatac
      301 cggcacggtc ggcaaggtaa tgcaaccggt taccctcctg caccagacgg gcgccgaaac
      361 agggcgtcac tgtgcagggt agtccccacc agggatggtc gtcattgtca tcgggatgcg
      421 ttgtcccgga aagtgtgtct gacacgataa aatcctcaca gaaaatcggt gaatgatagt
      481 ttaacgatga cgggtcagcc ggcgcgcaga ccggcttcgg taaaggtgta ttcgttgatg
      541 agcaggacct catccacagc ggcgtctgag ctctgccagt cgtactcgtt ttccgggtga
      601 cggaacatca gttcagggtg acgctcatca gccggacata cggaccaaaa ctgtccttac
      661 ggcgttctgc aaacacggcc agcacaccgg gaatatcctg cacttcacga ccggtatacg
      721 cttcagcact gccgtgccag cggtatttgc cggtacagaa cggaaaaaga cgggatgccg
      781 gatgctgacg gtgaatacgc atggcttcgc cacgggtgat gattttcata gtgggatacc
      841 tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg gttgtgacgg gttaaagcag
      901 accatattcg gcaaaggaga aaacctggct gccaccgact atcagatggt ccggcacccg
      961 gatatccacc aggcccagtg cctgtaccag acgttccgtg ataaggcggt ctgccttgct
     1021 gggtgtgact tcaccggacg ggtgattgtg tgccagcacc acagcagcgg cattgtgata
     1081 cagggcgcgt ttaatcactt cccggggatg gacttccgtg cggttgatgg tgccggtgaa
     1141 gagggtttca ccggcaatca gctgattctg gttgttcaga tacagcaccc ggaactcttc
     1201 acgctccagt cccgccatgt tcagaatcag ccattctcgt gccgcacggg tggaggtgaa
     1261 ggccacgccg ggttcatgaa gatggcggtc cagggttttc agggcccgtt gaatgaggct
     1321 gcgctcgccg ggcgtcatct ctccgggcag aaaggaaagc tgttgcattg tacttctctc
     1381 cattcagtcg atgatgcgca taatggcgct gcattccgga tgctgcaggg cgtaatcccg
     1441 cagccggtaa taatggaccg tcatggcata acactccgta cgacaggcat gatgactgta
     1501 cgtcatcaga caggcagcaa tgccggcggc ttccgggctc atttcagcgc ggttaccgtt
     1561 catggcattg aacagtaccc atgtttcgtc atcatcgtca tccggttcgg gtgccataaa
     1621 tgccccgccg ttgttcaggg tgtacagatt ccagatacca ccgcagtagt cttcacacag
     1681 acggtccatc cagccgaaga tacggggctc cagggtcacc cactgtggaa tgaggccaaa
     1741 gtgctgcggc cagaagctga tgcgccgttc atcagggacg ggagtggcta ccagtttttg
     1801 caggtcagtc tccggtgcac aggccgaaga ggtggtgttc tgagacaatg ttttcatgta
     1861 tgaaatcctt gtaatgtgaa taaatgagag ggagtaaagg tcgggagctg gcaacatcac
     1921 gttcatcact gaccgtgatg acccggtaat atcagggatg aatgata
//
LOCUS       DACADA010000329         1945 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.337, whole genome shotgun sequence.
ACCESSION   DACADA010000329 DACADA010000000
VERSION     DACADA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.337"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..354
                     /locus_tag="ISB17_004850"
     CDS             <1..354
                     /locus_tag="ISB17_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HAP1982675.1"
                     /translation="AITKDASTLNPEGYSVVEIPDITANRRADDSGKWMFKDGAVIKR
                     VYTEEELRLQTENQKKILLQQAREKTQFWQTQLTLGIITDSDRQQLMNWMRYVQQVET
                     TDTSVLPVTFPEPPE"
     gene            complement(385..912)
                     /locus_tag="ISB17_004851"
     CDS             complement(385..912)
                     /locus_tag="ISB17_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1982676.1"
                     /translation="MQHLKNITAGNPKTIEQYQLTKKAGVIWLYTEDGKNWYDELKNF
                     QDDTLKIAYDQKGIIRCIEKDVSTLNPDGLSVVELPNITANRRADISGNWKFLDGKVV
                     KREYTKQELQQQAELQKAALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVMV
                     SRVDTAKPEWPQKPE"
     gene            complement(928..1896)
                     /locus_tag="ISB17_004852"
     CDS             complement(928..1896)
                     /locus_tag="ISB17_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982677.1"
                     /translation="MTGELKIGTVNALRIFNDAFGLIFRRSEDFLHFIPTAEGQGENG
                     DIGPLRPFAINLRTGAISVSHGAKIDGGLALGTDNALGGNSITLGDNDTGIKQGGDGV
                     LLFYSNGQLAFGLQPASADFYKRVAYIHQGIIPDGSGAFADQLNNATAPFVQTQFAWN
                     PTPGGLYVPIVKGLSIRNGQGYPGAVSFGYLLTEQYGFPVPCIHMRGDGGNDALWQFN
                     PNDKSFISPGALIAGGVRYNTDGNIFGGCWGSNLNDYLNSSFIRNVRLGGRRSDTLYR
                     GGLCEPGNGHVTTGLQIIGEVDGDDWMVSRPLQKYISGNWYNVEQA"
BASE COUNT          529 a          487 c          400 g          529 t
ORIGIN      
        1 gccataacca aagatgcctc cacacttaac cctgaaggtt atagcgtcgt tgagattcct
       61 gatattaccg ccaaccggcg tgctgacgac tcaggtaaat ggatgtttaa agatggcgca
      121 gtcattaaac gtgtatatac cgaagaagag ttgcgcctac agacggaaaa ccagaaaaaa
      181 atattattac agcaggccag agaaaaaacg cagttctggc aaacacagct tactttgggg
      241 attatcactg attcagacag acagcaactg atgaactgga tgcgatacgt gcaacaggtt
      301 gagacaactg atacctcggt tcttcctgta acattcccgg aaccaccaga ataagaaaag
      361 gcccgtacgg gccttaattt gttgttattc tggtttctgt ggccattcag gctttgcagt
      421 atccacacgg ctgaccataa cgctgtagcg ttcccatgat tccagtcgtg tgcgttcctc
      481 atcagttgcc atattcagtc tgacagcacg ttccagcggt tgaatcacag actccgcttc
      541 ggaaagcaaa gcggcttttt gtaactctgc ctgctgttgc agttcctgtt ttgtatattc
      601 ccgctttact actttaccat ccagaaactt ccagtttccc gagatatcgg cgcgacggtt
      661 ggctgttata ttcggtaact caacaacact taacccgtca gggttaagtg ttgatacgtc
      721 tttctcaata caacgaataa tccccttctg gtcataagct atttttaaag tatcatcctg
      781 aaagtttttt aattcgtcat accagttttt accgtcttct gtatatagcc agataacgcc
      841 agcttttttc gtaagctgat actgctcaat ggttttgggg tttccggcgg taatattttt
      901 cagatgttgc atatattctc ctgatggcta tgcctgttca acgttatacc agttaccaga
      961 aatgtatttt tgtagtggtc gtgacaccat ccagtcatct ccatcaacct caccaataat
     1021 ttgcaatcct gttgtcacat gaccattacc tggttcgcaa agtcctccgc gatataatgt
     1081 gtcagaacgt ctgcctccca gacgcacatt tctgataaaa gaactattca ggtaatcatt
     1141 taagtttgac ccccagcacc caccaaatat atttccatcg gtgttataac ggacgccacc
     1201 cgcaataaga gcacccggtg aaataaagga tttatcgttc gggttaaact gccataaagc
     1261 atcattaccg ccatcgccac gcatatgaat acatggaacc ggaaatccat actgttctgt
     1321 cagtaaatac ccaaagctga ccgcgccggg atagccctgt ccattgcgaa tggacaagcc
     1381 cttaactatc ggcacgtaaa gaccaccagg agtgggattc caggcaaact gcgtctgaac
     1441 aaaaggcgcg gtggcattat tcaactggtc tgcaaatgcg ccacttccat caggaattat
     1501 tccctgatga atatatgcaa cccgcttata aaaatcagca gatgcgggtt gaagcccaaa
     1561 tgccagttgt ccatttgaat agaataaaag gacaccgtcg ccgccctgtt taataccagt
     1621 gtcgttatct ccgagagtaa tggaattacc gcccagtgcg ttatctgtac caagcgccag
     1681 cccaccatca attttggccc cgtggctgac agatatagca cctgttctca gatttatagc
     1741 gaatggcctt aatgggccga tatcaccgtt ttcgccttgt ccttcagccg tcggaatgaa
     1801 atgaagaaaa tcttctgaac gacggaaaat aagaccgaag gcatcattga aaattcgcag
     1861 cgcattcacc gtgccaattt tcagttctcc ggtcatttta tcgccggaac gctgaacggc
     1921 gttaccagcc ttgtttaccg tttcc
//
LOCUS       DACADA010000330         1933 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.338, whole genome shotgun sequence.
ACCESSION   DACADA010000330 DACADA010000000
VERSION     DACADA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.338"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(74..994)
                     /gene="speB"
                     /locus_tag="ISB17_004853"
     CDS             complement(74..994)
                     /gene="speB"
                     /locus_tag="ISB17_004853"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAP1982678.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1130..1861)
                     /locus_tag="ISB17_004854"
     CDS             complement(1130..1861)
                     /locus_tag="ISB17_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982679.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVHNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT          489 a          518 c          475 g          451 t
ORIGIN      
        1 tgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tgacacatca cccgtgcgcc
       61 tcttatctgg cgcttactcg ccctttttcg ccgcctgaat atacagcatc tccagcgcca
      121 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
      181 cgtccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg gcgcgatcgg
      241 aggtcaggcc gccaattacc ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
      301 cgatatcaaa ggtcaggtaa accggcatat cacccacaat ctgtttcacc tgggcgataa
      361 tgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
      421 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
      481 tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgca taggtatcgg
      541 tgtgggcgtc aaagtgtacc aacgccattt tgccgaaatg cttcgcatga gcgcgcagca
      601 gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca
      661 gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg aaggcatata
      721 ccagatcacc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc cacgggaagc
      781 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtgacgac
      841 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtcagcat
      901 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg ttggaaacca
      961 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctttta caaagtcgat
     1021 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca
     1081 agtttgtgct aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac
     1141 acggtggtgg cgcatagcca ttgttatctg ccggaaacgc ttgtccagag cacggcggca
     1201 gcgcgttttg cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattat
     1261 gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag
     1321 ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca
     1381 caatataacc gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa
     1441 ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggatatat aattgataat
     1501 tcattccgat tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc
     1561 gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac
     1621 gaatactata aacgcggtcg tagccggaag gaacattagt cgaataacta ataacgatca
     1681 tctctgccgg atcgcgccag gctcgttcag cagatacgcc ctgaaccgtt gagtgcacat
     1741 ccccacccca ggcggtgccg acattacccg atgcactgct attatactgc gcattactgg
     1801 aacaaccggc cagcagcacg gctaatgcta tcaacgcgct acgtaatttc cattttttca
     1861 taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt taataagaaa
     1921 agcagctaaa tta
//
LOCUS       DACADA010000331         1929 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.339, whole genome shotgun sequence.
ACCESSION   DACADA010000331 DACADA010000000
VERSION     DACADA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.339"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>1929
                     /locus_tag="ISB17_004855"
     CDS             <1..>1929
                     /locus_tag="ISB17_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1982680.1"
                     /translation="AGSQAGTVRVTAKVQGKTFTLSIKQTATTEPDSEVSAVLTAAPA
                     EQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYSATVTSK
                     RAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRNMVTVSL
                     ADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVITAHAGS
                     QMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNASVNGQKI
                     QLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGSVKLTFV
                     PDVASAELNMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTGASLTSS
                     NLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVLEITSSA
                     SSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTTDENGQA
                     TVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPGYETNDGSPV
                     GIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEFSTYESG
                     YHWIKAKITNSKNTYEKT"
BASE COUNT          568 a          375 c          480 g          506 t
ORIGIN      
        1 ctgctggttc gcaggctggt acggtacgtg taactgcaaa agttcaggga aaaacattta
       61 ctctcagtat taagcaaaca gcaacaacag aacctgattc tgaggtcagt gctgttttaa
      121 ccgctgcacc agcagaacag gtagttggtt acaatatcaa tctacaactg gcagtgaagg
      181 attcacaagg aaatgcaatc actggtgata acactctgag cttttatgct cttaaccagg
      241 cagaaggagt cgagtttggg actgtaacgg aaaaagacgg tgtctatagc gcgacagtaa
      301 cttcaaaacg agctggtaag attactatcg gtgttaaatc tgatagtcat gatttctcag
      361 gtgttaagaa agaaatcacc tttattgagg accggcagca ggttacattc tctcagtttg
      421 aagccagcca aaataatgca ttggctgatg gaaaacagag aaatatggta actgtaagcc
      481 ttgcggaccg tttcggcaat gttgttcctg gttatgctgt cacattgtct ttaccagcag
      541 ggataactca agttggtggt gaacatgctg tttcaactga cgaaaatggt aatgctattt
      601 ttgcgttaat aagctccact ccggggtctt acgtgattac agctcatgct ggttctcaga
      661 tgtctactga gttaacagtg acctttgcat caaatatgac cggagcttca ctgtcactga
      721 cgcctgaaag cagtagttta atttccaata ttcctgctaa tggtaaggat gcagcggtat
      781 tagatgtgca gctgacaaat acgaatgcgt cggtcaatgg gcaaaagatt cagctcatca
      841 cctcatctga aggattatct gtaccaacaa atatcgtgac tgattcaaca gggcatgttt
      901 ctgttccgct tacaacggtg aaagctggtg agtacacggt gactgcacgg gtgactgacg
      961 gttcccatag cgtggaatct ggcagcgtta agttaacttt tgtgcccgat gttgcttctg
     1021 cagaactgaa tatgagtgta tcaaaacagg agattgtggc cgatggcagt gaaaacgcaa
     1081 cagtaaacat tcagttggtt gatgcgaata acaatgcgtt tacaggcgag gtggatttaa
     1141 cgattacacc atcgacggga gcatcattga ccagtagtaa tctgcaactg gatgctcatg
     1201 gacaggcaac aacgcagttc actgcatcga aatcaggcca gtacacaatt caggccgagt
     1261 atgttctgga tgggaaacgc ataaccgcaa gccagaatat tgatgccgtg acggatgtga
     1321 agggggctgt gttggagatc acatcatctg catcttcggc tgtagtgagc gataccaaca
     1381 atctggtatt tacgttactg ttgaaaagta agtcaggcga agcattaagt ggtcgagcat
     1441 tgaatgttaa gacatctggg ccgtctaaat atggtgcgtt ggtggttgat aaaaccacag
     1501 taacgactga tgagaacggt caggctacgg taagcgtaca tggtcgaacg gttggttcgt
     1561 ataaattaac ggcgacctta aacgaacttg gcagtgatgt tagcgcggtg aaatcattct
     1621 ccctgtacgc tgatgacgca aatggagtat tgactctaac aaaagatcct ggttatgaga
     1681 caaacgatgg atctccagta ggtatttatg ctcgatttgt cgatcatttt ggaaacccat
     1741 tatctggtac ggttgagttt tctgctggta gtgaagatag tccagactct caacgggtca
     1801 aaatggagcc tgctacagta acgttacact ggacaggtaa tgcagcttcg gaattcagca
     1861 cctatgaaag tggttatcac tggattaagg ccaaaataac gaatagcaaa aatacatatg
     1921 agaaaacaa
//
LOCUS       DACADA010000332         1902 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.340, whole genome shotgun sequence.
ACCESSION   DACADA010000332 DACADA010000000
VERSION     DACADA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.340"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(517..642)
                     /locus_tag="ISB17_004856"
     CDS             complement(517..642)
                     /locus_tag="ISB17_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1982681.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGVTSRMLSVIPLLFFQG"
     gene            1136..1675
                     /locus_tag="ISB17_004857"
     CDS             1136..1675
                     /locus_tag="ISB17_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="HAP1982682.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFESSLKYSNHDSEQG"
     gene            1682..>1902
                     /locus_tag="ISB17_004858"
     CDS             1682..>1902
                     /locus_tag="ISB17_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982683.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCE
                     DKEFNLVDVINEDGNKIMFVVSNDEKIQKP"
BASE COUNT          580 a          382 c          456 g          484 t
ORIGIN      
        1 aaaaaaaata gcaaagaaaa aaactccagc acctgtaatt gagctagata aatatcgaaa
       61 aaaatgacca ttgagccagc ggcaggcgga caatcagggg ctacgtgtta acgttctgac
      121 cttgattgtc tatcctgcat tgctcttttg ccgcctcgaa tcccttgcgt gtttttgttc
      181 cacgctctcc agaacgaccg agccgccacg gttccgatag cgccttgagc gcaaccggaa
      241 accgtctggg gagaacccca gacccccatc gctgtaatac cgacggtcta cgaccaccta
      301 ccagccgaat agctgcctat gccgtgcaaa taggctttgc agggggctga cgctgttcct
      361 ggcgacctga aagcccctgg ttaggggtat cggcgcgcct gacctcctga gagcctctgt
      421 aagcgttttt cgcgccttcc atgccctgac gggcatttaa aacccgtttc gtgcgtctga
      481 gagcttctga gcgcgtttga agggctatct gacgactcat ccctgaaaga acaacaacgg
      541 gatcaccgac aacatgcgag aggtgacacc gctcgccgca agtcgatgac cgagcgtagc
      601 gagcgaatcg acgaggaagc ggaagagaac cggaagccac attgagcact tacgcactga
      661 tgcggggtgt cggggtgaag ccctgaccag gtggtgaatg tctgagtgcg cgtgcgcggt
      721 ccgacattcc cacatcctgt cccgatttct gaggcttttt agcggtgaac ggacggcaaa
      781 accatgatgg agttgttgaa cgttgagagc atgaaatctg gcaaaaatca cggctggtaa
      841 ggcttgcaga ggagtaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
      901 gcgccccgac accccaaaag cagttaggta gtgttaaatc atgagcagta aaaaaacgct
      961 ttgatagatc tttagagcaa aaattgacat ttctttaaaa atcattgact taagacacct
     1021 tcatacgaaa cgttatttgc atgtgtatac gcaaacatgt ttgcatgtgt tgtttcgtac
     1081 tgtacgatat aaatttcgta aggtgcgaaa tatcattgca ggtaaggatg caagtatgtt
     1141 ggaacagcaa tttgaagact ttgaacagca aaaacggcga gatcatcagg tacaagatca
     1201 atccaagaac gctaagcaaa aacgagatcg gttgaatcct ccagactggt cgcaggttac
     1261 taaggctggt tggaatatgt tggacgctat atcagaaaaa gttcctagag catttcggtt
     1321 atattctttt tttgcccaga atatagataa atcatgtggg gctgttgtat gtgatcaaaa
     1381 attcttagct gattatttcg gtgttagttt aagaacgatc caatattgga ttggagattt
     1441 agaaagtatt ggcgcaatag ttaaaatacc agtaggcata tattatgcct atgcattaga
     1501 tccttatcag gtatggcgtg gatataacaa tactaaaaaa tatgcagcat ttaacgcaaa
     1561 aacattaact caaaaagatg gggttataga tcgtaaattg cggcttatgc tttctaggcg
     1621 tgaagagttt gaaagttcct taaagtactc taatcatgac tcagagcagg ggtgatgcac
     1681 tatgagaatt aaatatgaat cacgttggag attagaagaa ctaaaattag aaatatgcag
     1741 ttcaatagat gagttaatta ataaatatgg tattgaatat gcaacaggaa taaatcttta
     1801 tatgaattgt gaagataaag aatttaatct tgttgatgtt atcaatgaag atggaaataa
     1861 aataatgttt gtcgttagca acgatgaaaa aatacaaaag cc
//
LOCUS       DACADA010000333         1849 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.341, whole genome shotgun sequence.
ACCESSION   DACADA010000333 DACADA010000000
VERSION     DACADA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.341"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1644
                     /locus_tag="ISB17_004859"
     CDS             <1..1644
                     /locus_tag="ISB17_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982684.1"
                     /translation="VIDTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATD
                     HLTVDSYSTVNLTESTGWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTS
                     GNIADHNGNVVSGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTING
                     NGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATF
                     SAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRL
                     TNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAA
                     AGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTY
                     VDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYD
                     SMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVG
                     LGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1730..>1849
                     /locus_tag="ISB17_004860"
     CDS             1730..>1849
                     /locus_tag="ISB17_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1982685.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVN"
BASE COUNT          498 a          426 c          441 g          484 t
ORIGIN      
        1 gtaattgata cattagctgt aaataatggc tctcaggtaa atgttactga ttcctctctg
       61 gtctcagata ccattggttt aaccaacggc tcctcactga atattggtga agatggtttg
      121 gttgctacag accacctgac tgttgatagc tacagtaccg ttaatttaac cgaatctact
      181 ggctggaata actattctaa cctgtatgcc aataccatca ccgtaactaa cggtggtgta
      241 ctggatgtga acgttgatca gtttgatact gaagctttcc gtactgacaa actggaactg
      301 actagcggca acatcgctga ccataacggc aacgtagttt ctggtgtgtt cgacatcaac
      361 agcagcgatt acgttctgaa cgcggatttg gtgaacgacc gtacgtggga tacttccaag
      421 tctaactacg gttacggtat tgttgctatg aactctgatg gtcacctgac tatcaatggc
      481 aacggcgacg tagacaacgg tactgaactg gataacagtt ctgtagacaa cgttgttgct
      541 gcaaccggta actacaaagt tcgtatcgac aacgcaactg gcgctggcgc aatcgctgat
      601 tacaaagata aagaaattat ctacgtcaac gacaaaaaca gcaacgcgac tttctctgct
      661 gctaacaaag ctgacctggg tgcatacacc tatcaggctg agcagcgtgg taacaccgtt
      721 gttctgcaac agatggagct gaccgactac gctaacatgg cgctgagcat cccgtctgcg
      781 aacaccaata tctggaacct ggaacaagac accgttggta ctcgtctgac caactctcgt
      841 catggtctgg ctgataacgg cggcgcatgg gtaagctact tcggtggtaa cttcaacggc
      901 gacaacggca ccatcaacta tgatcaggat gttaacggca tcatggtcgg tgttgatacc
      961 aaaattgacg gtaacaacgc taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt
     1021 gatatgaatg accgttctgg tcaggtggat caagacagcc agactgccta catctactct
     1081 tctgctcact tcgcgaacaa cgtctttgtt gacggtagct tgagctactc tcacttcaac
     1141 aacgacctgt ctgcaaccat gagcaacggt acttacgttg acggcagcac caactccgac
     1201 gcttggggct ttggtttgaa agccggttac gacttcaaac tgggtgatgc aggttacgta
     1261 actccttacg gcagcgtttc tggtctgttc cagtctggtg atgattacca gctgagcaac
     1321 aacatgaaag ttgatggtca gtcttacgac agcatgcgtt atgaactggg tgtagatgca
     1381 ggttatacct tcacctacag cgaagaccag gctctgactc cgtacttcaa actggcttac
     1441 gtctacgacg actctaacaa cgataacgat gtgaacggtg attccatcga taacggtact
     1501 gaagggtctg cggtacgtgt tggtctgggt actcagttca gcttcaccaa gaacttcagc
     1561 gcctataccg atgctaacta cctcggtggt ggtgacgtag atcaagactg gtccgcgaat
     1621 gtgggtgtta aatatacctg gtaatattct tcactccgaa gaaataggta atttaatcta
     1681 aataatgccc gtcaaggatt tgacgggcat tactgcaaag gacgcgcaaa tgttatctgt
     1741 agttaaacct cttcaggaat ttggtaagct cgataaatgt ttgtccagat acggtacgcg
     1801 cttcgagttt aataatgaaa agcaagttat attttccagt gatgtcaat
//
LOCUS       DACADA010000334         1811 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.342, whole genome shotgun sequence.
ACCESSION   DACADA010000334 DACADA010000000
VERSION     DACADA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.342"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            51..305
                     /gene="bssS"
                     /locus_tag="ISB17_004861"
     CDS             51..305
                     /gene="bssS"
                     /locus_tag="ISB17_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAP1982686.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            420..1538
                     /gene="solA"
                     /locus_tag="ISB17_004862"
     CDS             420..1538
                     /gene="solA"
                     /locus_tag="ISB17_004862"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAP1982687.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1586..1699
                     /locus_tag="ISB17_004863"
     CDS             1586..1699
                     /locus_tag="ISB17_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAP1982688.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          463 a          445 c          435 g          468 t
ORIGIN      
        1 gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg atggaaaaaa
       61 ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc gttgatagct
      121 atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag caggaaggga
      181 ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt atttcgatat
      241 tagaagcagg aatcgccaaa attgaatccg gtgatttcca ggtaaacgag tatcggcgtc
      301 attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta ccatttctgg
      361 gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga gagaaaaaaa
      421 tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc gggtattatg
      481 caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg catcaacacg
      541 gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc gaaaagtatg
      601 tcccgctggt cctccacgcg caaatgctgt gggatgaact ttcccgccac aacgaagatg
      661 atcccatttt tgtacgctct ggtgtcatta acctcggtcc ggctgactcc gcatttctcg
      721 ccaacgtcgc ccacagcgcc gaccagtggc aactcaacgt tgaaaagctc gacgcgcaag
      781 ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggt ttatttgaaa
      841 ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa ctggcgaagg
      901 aagcgggctg tgcgcagctg ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg
      961 gcgtaactat tgaaacggca gacggtgagt atcaggcgaa aaaagcgatt gtctgcgcgg
     1021 gaacatgggt aaaagaccta ctcccggagc tgcctgtcca gcccgtacgc aaagtatttg
     1081 cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggca tttaccggtg
     1141 agctgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg ttgaagattg
     1201 gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg tttgcggaag
     1261 tggccagcga tggttcggaa gccttcccgt tcttgcgcaa tgtgttgccg ggtatcggtt
     1321 gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat tttattatcg
     1381 ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg cacggtttta
     1441 aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagacaaa aaaagcgatt
     1501 ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg cctccagaat
     1561 gaggccgttt ttacaggagt atttaatgcg tcgactgttg cactatctca tcaataatat
     1621 tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta tggatctgat
     1681 ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt taatctccct
     1741 ttcatttagc gtgaaaaatt gaatgagatt cttcctgcct ctacagcact cactagacct
     1801 tccagatgct t
//
LOCUS       DACADA010000335         1763 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.343, whole genome shotgun sequence.
ACCESSION   DACADA010000335 DACADA010000000
VERSION     DACADA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.343"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            36..1565
                     /locus_tag="ISB17_004864"
     CDS             36..1565
                     /locus_tag="ISB17_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP1982689.1"
                     /translation="MYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIPIIKTILDFI
                     VSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLL
                     VVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLRIPESLGFYSGPRL
                     VPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLN
                     HLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYG
                     LAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFLFVSPLLFVFHAFM
                     NGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFFIYYFVFRWT
                     ILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLD
                     NCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGI
                     Y"
BASE COUNT          427 a          347 c          433 g          556 t
ORIGIN      
        1 cctggagttt ttttcagagc ctggggaagg catttatgta tcccattgct ctgctaagtg
       61 tatgtggcat gatgctaggg ctgggaagtg gtttagccag tgatgatatg gcaaagttaa
      121 ttccatttct ggctatccca ataattaaaa ccatacttga tttcattgtt agtcttggtt
      181 tgtttgcctt tgttaattta cctgtattgt ttgcgatagc gattccctta ggattattaa
      241 aagataaaga ggataaagcc tatggtgctt tttctggctt aattggtttt atggcgatgc
      301 atctgggaac gaacttttat cttaaacagc acgacttatt ggtcgttgct gaccaaatgt
      361 cgacacatgg gcaaaccatc attctgggga tccagtccta caataccagc gtgttggggg
      421 gaattgttgc tgggttatta gtcgccagca tgtataaaaa gatcgttaat ttacgcattc
      481 ctgaatcgtt aggtttttat agcggcccac gtctggtgcc tatcattaca ctgattgtga
      541 tgagtggatt tggtctgatc attcctttta tctggccgcc gtttttcaat cttttcatgc
      601 tcattggaca ctggatttca acttccggtc ctgttggtta tttcttctat gcagttgccg
      661 aacgcgtgac gattcctttt ggcttaaacc atctggtgac gtcagttttc cgctttacgc
      721 caatcggcgg ttcggctgtg attggcggcg aagaatatta cggcaccctg aacatgttta
      781 tggcgtacgt caaagagaat gcggtcattc cgctggattt ggcggggaaa atggagcagg
      841 gcaaactgat gattcagtat ggtctggctg gtgccgcgct ggcgatgtat cgcactgctc
      901 atgctcaaaa cagaaaggct atcaaagcat tgcttatttc cggggtgctt acggtgatta
      961 ttggcggcgt cagcgaaccg attgagtttc tgttcttatt tgtcagtcca ctgctgtttg
     1021 tcttccatgc ctttatgaat ggattcgcta acatggttct gccatatatg ggcgtgaaga
     1081 tgggatttac tggcgatctg attcaattta ttagctttgg cgtattgcgt ggcacaagaa
     1141 caggttggcc gatcgcggtg tgtgtcgaag tggcctattt cttcatttat tactttgtgt
     1201 tccgttggac cattcttaaa tttaacctga tgaccgtagg ccgtgaagag tccagtcctg
     1261 tcacgctgaa cgctcacgaa gatacggcta tagcggatat cccaactcct gataaatcag
     1321 agctgcaagc ggcggagcag atggttaagg cacttggtgg taaagagaat attaagtcac
     1381 tggataattg cgtaactcgt ttacgtttaa caatcgcaga tatgggattg attgacgaag
     1441 ttgcaataaa aatagctggc gggattgcgg ttgttaaact tgatcaaaat accctacaag
     1501 tcattatcgg cactaaagtc atcgccctgc gtcgggatat ggataactat atggggatat
     1561 actgatggat gtttttaata ctcctgtttc ccgcaaagga acatattgta cccaatggga
     1621 cttttgcgag gacagatttg gtgtaaaaga tgtactacca ttctctattt cagatatgga
     1681 tcttccgata ccagacgcta tcaccagagc attaaaaaaa cgcctggaac acccgatttt
     1741 agggtacagt cgttggcaac atg
//
LOCUS       DACADA010000336         1762 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.344, whole genome shotgun sequence.
ACCESSION   DACADA010000336 DACADA010000000
VERSION     DACADA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.344"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..236)
                     /locus_tag="ISB17_004865"
     CDS             complement(<1..236)
                     /locus_tag="ISB17_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1982690.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKL"
     gene            785..1534
                     /locus_tag="ISB17_004866"
                     /pseudo
     CDS             785..1534
                     /locus_tag="ISB17_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          478 a          390 c          451 g          443 t
ORIGIN      
        1 tcaagtttat tttttcctcc caatgaagtg attcggaaat gagaacctcc tgcctgctcc
       61 gctttgtctt taagcgcagt tgccacctct tctggtgtat atagattacc aacagaaata
      121 tagtctgacg gcatcaaacc aacaacgtcc tgacgattaa tttcatctgc agcagttgcg
      181 tagcctgtca gaatgagacc aagggcgaca atggtagtgc agataacata attcatgagc
      241 aatttctctt tatgtttgcg atactacaca tgctaccttc tgattggtag gcttgcaaga
      301 tggatttatt taaaataaga gggatgattt gtatcaaatt gttttgtatt tttgtgtttt
      361 gtatcactga ggtgaagttg taagatctgg ccctctgcga ggttggctct gtcgaaagga
      421 acaggtgacg agagttaaca tcgttgcctt ggctgcatgg gggatatgta gctaccccct
      481 gatatttccg ccacggaagc caatatgaac aacaataaca atacgcctcg tttccagtat
      541 gcttcatgtc ggatattttg taagcgctat gtacttcccg ggacgcttat cttttagccg
      601 tagctggtag tgcaataaaa gcctggcagc aatccaggcc tctacgttct aagaatcaac
      661 tgtcagtgca gaggaatgcg cacgacgctc tcgttgttat tggcttttgc cgtcgcaccg
      721 tcgtttttca cggtgacaaa cagcgcgtgc gctttgctat ccgtcttata tcactggtgc
      781 tgacaatgtg gatgaggtcg cctttaaaaa aggacatcgt tatataacgg tgatctccga
      841 tcgcgatggt cgtgcgctgg ccttaacgga cgatcgtggc acagagagcc tcgccggcta
      901 tcttcgcacg cttactgacg ggcagttgct ggctatcaaa acgctctcaa tggacatgaa
      961 tgcaggctat ataagagcag cgcgtatcca cttaccctgt gcggttgaga aaatcgcctt
     1021 cgaccgcttc catgtagcaa agcaacttgg cgaagtggtt gataaaaccc gtcagaatga
     1081 acatccgcac ctccctgttg aaagttggcg tcaggcaaag gggacccgct tcctgtggca
     1141 gtacagcgat aaatggatga ccaaatccag gcaggaaaag ctgatatggc tgcgtgcaca
     1201 gatgaagctg acaagccagt gctgggcgct gaaagagctg gcaaaggata tctggaacag
     1261 gccatggagt gaggaaagac gtaatgactg ggagagatgg ctggctctgg cggccaacag
     1321 tgacgttcct atgatgaaaa acgccgcgaa aacgatagga aaaaggcttt acgggatcct
     1381 gaatgccatg cgacaccgtg tctcaaacgg aaacgcggag gcgcttaaca gtaagatcag
     1441 gctactgagg ataaaagcca ggggataccg aaatcgggag cgctttaagc tgggcgtgat
     1501 gttccactac gggaaactga atatggcgtt ctgagccttc ccaccatgat cagggaaaac
     1561 ccatgtatca tcaggttatt atgtgagctg gtaaatcaga ttttgcatgg actgaaatca
     1621 tgctgttatt tagggataaa gaaccggctt tttgtcccgc cccctcgcca ttaatgaaaa
     1681 aaagaatgga atgctccctc tgaggagaag ggcagtagaa aaaagaaccc gccagcaaaa
     1741 atattgggga tgaacagctt tc
//
LOCUS       DACADA010000337         1748 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.345, whole genome shotgun sequence.
ACCESSION   DACADA010000337 DACADA010000000
VERSION     DACADA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.345"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..1695)
                     /gene="hcp"
                     /locus_tag="ISB17_004867"
     CDS             complement(<1..1695)
                     /gene="hcp"
                     /locus_tag="ISB17_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP1982691.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFVQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEF
                     REALWLEVSQEPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIA"
BASE COUNT          455 a          440 c          404 g          449 t
ORIGIN      
        1 agcaataaaa tgatgtatct gaaatttttt aagtgtttct ttaggaccaa tatagtgtcc
       61 ttcgtcgggg gcaaaaggtg taaagccctg actaatacgt aactgattaa tttccgaaaa
      121 ctgcgctatc aactccggtt cctgactcac ctccagccac agcgcctcgc gaaactcatc
      181 aaaggtttta aactcccgtc cgcgcaattt atcaacaata tgcgccggga ttggtgcgcc
      241 gttgccgtta ttggataccg acatatccag ccagcgagtt cctggtgaaa gcgggacgcc
      301 gtgccctgtt acaacaccag gcagctttcg cggatcctcc ttcaggtaaa catagatagg
      361 cttaataccg gatcccgccg gaaaaaccag aatataatcg ttgaattctt tcggatcagg
      421 aaccgggaat ggcggcgtgg tataggtatc ggtgccgtcc ggaatcggcg tcaccagaat
      481 ggtgggctgc tccagcggcg gcctgtcgtt accggtatgg cccggaacat tcccatgttc
      541 cggattttcc ggcgtccagg taatcagggg gccatcgaca tcgtgagcgg gggtaaattc
      601 ataacgctgc gtctgctcgt tccactgcat gttcgccacg cggacgtttt catacggcgt
      661 accttcaccc gtatgaacac catacacctg aggcttgccg tgaatgtcag ttccccagaa
      721 aaaacgcacc cgggtggtgg cggtactgcc tgctagtgct ttctgccgca gttcttcctc
      781 ataccagaac tcatcacgtc caggcacacg agcactgccc tcacccgcag agggaatata
      841 cagagcagat gccaacgcac cgatgtaaga gccgcgggtt gcactgagta cgccactgag
      901 cgaccatttt ccccatattt gagtcgccaa cacactactc gggaacagta gcatggtttg
      961 agcttctgcg ggttccccga taacgttagc ctcgggatgg tggtatccac agcagtcatc
     1021 aaccactggc tgaacgaaaa tcgctacttg accgaagttt tcagcgggtt cttctgccgt
     1081 tccggcatct gtacatccct tcaccttcag acaggatttg gcatagaccg ggttaaccgc
     1141 tggtgaagag agtggaaggt taacaactgg tagcggttca ggcttaagat tactggcagg
     1201 tggactttta ggcggaataa ataagctatt atagccagca ggaaaggcca accagtcaaa
     1261 ttccgtattg gctatgagat gtttacataa tatgtagtca taatcgacag ttacacattc
     1321 ggtaggaaga ttattgtcag aaatgtttac atgaatagca gttaatgaag catttcttag
     1381 ctgaatataa taataccgtt cccatcgccc gcttttattt atacgataca aatcgatttc
     1441 aataaataac cgctcattct gattgatggc attagacagt agcggtgagc ttttatcaat
     1501 gagtttgcaa aattgtagcc cctgtagatt aacgccatta ccagtactgg taatggcgtt
     1561 ggtgagtgca aaggcaaaaa tttcatcttc atggccaacc tgccagcgat taccaaccga
     1621 cgcatacgtc ccacagcctg aagaaatatc gccctgcttc tcaccgatta ttctcaggta
     1681 aacaatatcg cccactttat ttcattcccg tttatcctta acttatttgc attaaataga
     1741 aaacttat
//
LOCUS       DACADA010000338         1736 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.346, whole genome shotgun sequence.
ACCESSION   DACADA010000338 DACADA010000000
VERSION     DACADA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.346"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..745)
                     /locus_tag="ISB17_004868"
     CDS             complement(<1..745)
                     /locus_tag="ISB17_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1982692.1"
                     /translation="MRLTSRFGYVNQIRRDRPLTHEELMHHVPGIFGEDRHTSCSERY
                     TYIPTITVLESLQREGFHPFFACQTRVRDPDRREYTKHMLRLRRNGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIH"
     gene            complement(966..1376)
                     /locus_tag="ISB17_004869"
     CDS             complement(966..1376)
                     /locus_tag="ISB17_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982693.1"
                     /translation="MKTFIKTLLVAVTILFSVFAAAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKEQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(1392..>1736)
                     /locus_tag="ISB17_004870"
     CDS             complement(1392..>1736)
                     /locus_tag="ISB17_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982694.1"
                     /translation="YELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVT
                     INGTVWLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEF
                     AYLVKNYVAAVN"
BASE COUNT          401 a          438 c          439 g          458 t
ORIGIN      
        1 tgtgaatggc acgggtgtgg atacgttttc cttttgcact gcgaccggaa atcccgcctt
       61 tcagcatatt ctcctggatg gtctgataag tactccacag gtccttaccg taatcctccc
      121 ggcgtcgtgg cgtcagaatg tcggcggtgg tgacgggccg atgttcgtca ccataacggt
      181 aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcagaatc agcgactgca
      241 tggcatcacg cttctcctct atccggtcaa acacgcccac cacctcgtaa gccccttcaa
      301 tgactctgtc caccacattt ccccggtgcg gaacacgcac ttcccccaga gactgaccac
      361 agacgcatcc gttctggcag acgaacctga agtaacccgg cagcatctgg tagctggagg
      421 taccgtcatg agagttgagc agaataattt cagggacatg ttctccgttt atctctccgt
      481 tacggcgcag acgcagcatg tgtttggtgt attcccggcg gtccgggtca cgtacgcggg
      541 tctggcaggc gaagaatgga tgaaagcctt cccgctgcag gctttccagt acggtgatgg
      601 tggggatgta cgtatagcgt tcactgcagg aggtatgccg gtcttcaccg aaaatacccg
      661 gtacatggtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc tggtttacat
      721 aaccaaaacg actggttaat cgcatgattt gctccttatc ggtggttaag atttactggt
      781 gtaataaatg aaaaaagcca cgtctcccgg agaagacgcg gcctgacggg tgaaatgaat
      841 gaggtttatt gtctgagaag cctttcactg gcgagccacg cattaagcgg ctttccggca
      901 acaatggctg gggctgtatt tacctttgat attaataatg tgcagagaaa cgcatggttt
      961 actcctcagg ttttaagttt gtccagagcc acaacgacag gttttccgga tgtgtcgatg
     1021 actcctaccg tcggtacgcc atttttgatt tcgaagaacg gtttgttgct acctgagcac
     1081 agctggttgt ctttgtattc gagcatctgt tctttcccgg ttttcaggtt aagtaccgag
     1141 acagtgtagt cgtaacacca ggcaccacca cgttcaagca cgaaaacata gctgttatct
     1201 ttatagttga acggattctt cgtctggcaa ttcataccgt caatctcagt acaggaaaac
     1261 gcctctgtag tattaacgta tttgatgttg tttggcagtt ttacttgttt cgccgcagcg
     1321 aagacagaga acagaatagt tacagcaacg agtaaagttt tgataaaagt tttcatgaca
     1381 gactccttaa attagttaac cgcagctacg tagtttttca ccagatacgc gaattcatgc
     1441 acgccaattt cgccgaacca aaccaggtag gccgggtcat ctaaaccaaa gtagcggtag
     1501 ccaacgactt catgggtttt tgggttatag agtgttacga taaagtccca gccgcaggta
     1561 ttggctttgc aaccgtcgcc ctggagccag actgtaccgt taatggttac tggcgttacg
     1621 tttccgccag ggccatccat atacccgcct ttaccgaaca tgccgctcag tccgaccggt
     1681 tttaatgctt tgtcaagcgc agctttgtag gcagcgtcac cttcgtacaa ctcgta
//
LOCUS       DACADA010000339         1712 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.347, whole genome shotgun sequence.
ACCESSION   DACADA010000339 DACADA010000000
VERSION     DACADA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.347"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            263..1576
                     /gene="gltP"
                     /locus_tag="ISB17_004871"
     CDS             263..1576
                     /gene="gltP"
                     /locus_tag="ISB17_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAP1982695.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
BASE COUNT          367 a          395 c          479 g          471 t
ORIGIN      
        1 agtattattg cagattaaac aaataaaaat ccttctataa caaatggtta ttcattaatc
       61 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
      121 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
      181 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
      241 ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct ggcagattct
      301 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
      361 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
      421 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
      481 tgccaaacag ctcgggcgta ttggtgcgaa aaccattatc tacttcgagg tgatcaccac
      541 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
      601 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
      661 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acatcgtggc
      721 gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg
      781 gctttcatcc ctgcccgcga cgcatcgtga accgctggtg accgttttcc gctccatctc
      841 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcgccggtgg gtgtgtttgc
      901 gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact
      961 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtcgtactgg gaattgtggc
     1021 gcgcctgtgc gggttaagtg tctggatcct gattcgtatt ctgaaagatg agctgattct
     1081 ggcgtactct actgccagtt ctgaaagcgt gctgccgcga attattgaga agatggaagc
     1141 ctacggtgca ccggcgtcga tcaccagttt cgtggtgccg accggttact cttttaacct
     1201 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
     1261 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
     1321 gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg
     1381 tattccgctg gaaggtctgg cgtttattgc cggtgttgac cgtatcctcg atatggcgcg
     1441 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
     1501 caaatttgac cgtaaaaaag cgctggccta cgagcgtgaa atgttgggca aatttgataa
     1561 aacagcagat cagtaattga agattgccgg ggggatatcc acccggcaat gtgtggatgc
     1621 ctgactgagt aatagcctga tgtagcgtga tttttgtagg tcggataagg cgtttacgcc
     1681 gcatccgaca tcaacgcctg atgcgacgct ta
//
LOCUS       DACADA010000340         1685 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.348, whole genome shotgun sequence.
ACCESSION   DACADA010000340 DACADA010000000
VERSION     DACADA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.348"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..1667
                     /locus_tag="ISB17_004872"
     rRNA            <1..1667
                     /locus_tag="ISB17_004872"
                     /product="23S ribosomal RNA"
BASE COUNT          425 a          373 c          532 g          355 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      601 taattgatgg ggttagcggt aacgcgaagc tcttgatcga agccccggta aacggcggcc
      661 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga
      721 atggcgtaat gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg
      781 aagatgcagt gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac
      841 actgaacatt gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt
      901 ctgcatggag ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtgggcccc
      961 ttaccggggt tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag
     1021 agtaacggag gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat
     1081 ggcataagcc agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag
     1141 tgatccggtg gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat
     1201 aacaggctga taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg
     1261 ctcatcacat cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg
     1321 gtacgcgagc tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct
     1381 ggagaactga ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt
     1441 tcgggttgtc atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa
     1501 agcatctaag cacgaaactt gccccgagat gagttctccc tgactccttg agagtcctga
     1561 aggaacgttg aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag
     1621 ctaaccggta ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga
     1681 agaga
//
LOCUS       DACADA010000341         1668 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.349, whole genome shotgun sequence.
ACCESSION   DACADA010000341 DACADA010000000
VERSION     DACADA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.349"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            31..1259
                     /locus_tag="ISB17_004873"
     CDS             join(31..346,346..1259)
                     /locus_tag="ISB17_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982696.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVIPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRADDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRVYRTMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQWASDGLSDNRCLEI"
BASE COUNT          430 a          386 c          465 g          387 t
ORIGIN      
        1 ctagttttta gactagtcat tggagaacag atgattgatg tcttagggcc ggagaaacgc
       61 agacggcgta ctacacagga aaagatcgct atcgttcagc agagctttga accgggaatg
      121 acggtctccc ttgttgcccg gcaacatggt gtggcagcca gccagctatt tctctggcgt
      181 aagcaatacc aggagggaag tcttactgct gtggctgccg gagaacaggt cattcctgcc
      241 tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgtctgct cggcaaaaaa
      301 acgatggaaa atgaactcct taaagaagcc gttgaatatg ggcgagcaaa aaagtggata
      361 gcgcacgcgc ccttattgcc cggggatggg gagtaagctt cgtcagccgt tgtctccggg
      421 tgtcgcgtgc gcagttgcac gtcattctca gacgagccga tgactggaag gacggccgcc
      481 gcagccgtca cacggatgat acggatgtgc ttcgccgtat acaccatgtt atcggagagc
      541 tacccacata tggttatcgt cgggtatggg cgctgcttcg cagacaaaca gaacttgatg
      601 gtatgcctgc gatcaatgcc aaacgtgttt accggaccat gcgccagaat gcgctgttgc
      661 ttgagcgaaa acccgctgta ccgccatcga aacgggcaca taccggcaga gtggctgtga
      721 aagaaagtaa tcagcgatgg tgctctgacg ggtttgagtt ccgctgtgat aacggagaaa
      781 aactgcgggt cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca
      841 caacgggtgg cttcgacagt gaaacagtac aggacgtcat gctgggagca gtggaacgcc
      901 gctttggcag cgagcttccg gcgtctccag tggagtggct gacggataat ggttcatgct
      961 accgggcgaa tgaaacacgt cagttcgcca ggatgttggg acttgaaccg aagaacacgg
     1021 cagtgcggag tccggagagt aacggaatag cagagagctt cgtgaaaacg ataaagcgtg
     1081 actacataag tatcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
     1141 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
     1201 aatatctgcg gcagtgggcc agtgatgggt taagtgataa caggtgtctg gaaatatagg
     1261 ggcaaatcca ttgtcagcgc caacaatgtg ggctgacatg ataaagctgt ctaggaaatc
     1321 gttcgcctcc tcagcggaca atccaaatgg tgattgtctc tgttaaacgt ttattttgaa
     1381 ggtcgactga ataaggtgat gacgctgtag aatttttcac gtgccacaga attttgaacg
     1441 ctttctctta caatatttca atgtttctat cagtattcgc cggaagaagt catcgaccaa
     1501 atcatcccag tcgtctcgca ccactgacca ttcatggttg acatgtggtg gaaatccgct
     1561 tctacagtaa ccatttttta ttcgcaaaac cgaacacgcc atacggatag ctgttaactg
     1621 gcatgccgag gcagtcgtta tttatattta gttttgtcaa taatcttt
//
LOCUS       DACADA010000342         1667 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.350, whole genome shotgun sequence.
ACCESSION   DACADA010000342 DACADA010000000
VERSION     DACADA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.350"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            27..1133
                     /locus_tag="ISB17_004874"
     CDS             27..1133
                     /locus_tag="ISB17_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP1982697.1"
                     /translation="MLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVG
                     LVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIP
                     ELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGV
                     ELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIG
                     YRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELI
                     LNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWVTLAESGRSHEIQ
                     TALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          497 a          361 c          361 g          448 t
ORIGIN      
        1 aacacccatc attgttttct ccttttatgc tgacagaaaa aataaagcta cgtaatcgta
       61 tcgttatggc tcctatgaca acctggtcag ctaatcctga tggaaccatc tctgagcaag
      121 aactcgaatt ttataaacgt cgctcacaaa atgttggttt agtaataaca ggatgtacct
      181 atgtcacgcc aagcggcatt gggtttactc atgaatttgc cgcgtatgac gaccgtttca
      241 ttaacagcct tgaaaagtta gcagctgcag ccaaaagtgg cggtgctccg gctattttgc
      301 agattttcca cgccggtaat aaagccatcc cggagcttgt gccaaattac gatgttatta
      361 gtgccagcgc atcaagcgta aaatctggtg actttatgaa gagagtggta caaagcaggg
      421 aaatgactga aaacgaaatt caggaaacca ttagagcatt cggagatgtc acaaaaaggg
      481 cgattaaagc aggttttgat ggcgttgaac ttcatggtgc gcacggattc cttctgcaga
      541 atttcttctc ccctctattt aaccagcgta atgaccgatg gggaggagat cttgaaggac
      601 gtatgcgctt cccgctggcc gtattacagg aagtaaaaaa tgtagtctat gaatatgcga
      661 caaagccctt tgctatcggt tacagaatat ctcctgaaga atctgtaaca gggggactgc
      721 ggatagagga tacttataaa ctccttgatc ggctcatttc atccggtatt tcgtatatac
      781 atacctcgct ggtcagtatt aacgatagct accctgttga gtcgccaaat ggtccccgaa
      841 ctattgaatt gattttaaat catattgcag gcagagtgcc ggtaatcgcc gcaggtaaaa
      901 tacgcacacc gtctcaggct caagaagcaa tttccgctgg cctgccactt gttgcgatcg
      961 gtaaagggct ggtgataaat ccagaatggg tgactttagc tgaatcaggt agaagtcatg
     1021 aaattcaaac agcccttaat ccacaacgcg ttcctgaatt aaccattccg gataaactat
     1081 gggatcaaat tcaggcatcg aaaggaactg gctggtttcc gttaatggat taaatatcga
     1141 ttactctccc gagagtaaaa ctatcaaatc aatgagtaac cgatacagcc tcgcaaattc
     1201 tttcagataa ttaacccgtc actgcggcac gttctctcgc aaggcgtgcc tgcagtgtaa
     1261 tccagtatgt atccggcata gttgacggtt taatagactc aattcctctc cctggttacc
     1321 tacaacgata tcttgtataa caccccagga gatacgacgt tacgatgaga tgcgatatgc
     1381 tcctcagcgc caatcaacgc caggactggg ccagtcttga gatcagactc attcccgtaa
     1441 ctgatttcag gcgacatata tcaatgacgt tggggcaata aaagatagcc gatgtaatac
     1501 atctattcaa atttgtgata catgtcaaaa aattggttga ccaaactcgt tatttatata
     1561 agggcactta cgaagtgcac tcttttttaa agcgaggaag taccaatgaa agagaataaa
     1621 gtacagcaaa tcagtcataa actgattaat atcgttgttt ttgtcgc
//
LOCUS       DACADA010000343         1658 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.351, whole genome shotgun sequence.
ACCESSION   DACADA010000343 DACADA010000000
VERSION     DACADA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.351"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            61..495
                     /locus_tag="ISB17_004875"
     CDS             61..495
                     /locus_tag="ISB17_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982698.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            850..1230
                     /locus_tag="ISB17_004876"
     CDS             850..1230
                     /locus_tag="ISB17_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1982699.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1227..1574
                     /gene="tnpB"
                     /locus_tag="ISB17_004877"
     CDS             1227..1574
                     /gene="tnpB"
                     /locus_tag="ISB17_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1982700.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          392 a          415 c          467 g          384 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cagagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccattgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtacc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
      901 cccgaattta aacagctgct cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa
      961 cttgctcttg aaaatggcat taacgccaat ctgttgttca aatggcgaca acaatggcgc
     1021 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
     1081 gcagctgccg aacagccaga atcgctcgca gaggacccgg aaaccctcag tatcagctgt
     1141 gaggtaacgt tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg
     1201 actctgctga tacaggaact gaagcgatga tcccgttacc ttccgggacc aaaatttggc
     1261 tggttgccgg tatcaccgat atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa
     1321 cggcgctgaa agacgatccc atgtccggcc atgttttcat tttccggggc cgcagcggca
     1381 gtcaggttaa actgctgtgg tccaccggtg acggactgtg cctcctgacc aaacggctgg
     1441 agcgtgggcg cttcgcctgg ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc
     1501 agctggcgat gctgctggaa ggtatcgact ggcgacagcc caagcggctg ctgacctccc
     1561 tgaccatgct gtaaatctct ttatcctggt tgtcacagaa taagcccggt aaaatacggg
     1621 cttatgaacg acatctcttc tgacgacatc ttcctgct
//
LOCUS       DACADA010000344         1621 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.352, whole genome shotgun sequence.
ACCESSION   DACADA010000344 DACADA010000000
VERSION     DACADA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.352"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(59..1597)
                     /gene="cadC"
                     /locus_tag="ISB17_004878"
     CDS             complement(59..1597)
                     /gene="cadC"
                     /locus_tag="ISB17_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
                     /protein_id="HAP1982701.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          473 a          335 c          346 g          467 t
ORIGIN      
        1 ggaacataca aaaactcaac aacaaatatt cccgagcatg aatcaacccg ggagttactt
       61 attctgaagc gagaaatttg tcgagataag gtacaacata aggaacagaa gtctggaata
      121 taccattttc aatccagtaa agggtgtttg cccctgggcg taaattaaag gcggtgagat
      181 atgcatcagc tgcttcccgg ttcatcccct tcatttcata aaccttgcca agcaacacat
      241 aatttagcca ggacatttca agatcaatgc cggtatttat cgcctgataa gactcatctg
      301 ttttaccttt taccagggca ctgaccgctt ttatttgata tataatggac aggttgttca
      361 attccggcag tgtaacaatg ttatctattt ctgtgttcag tgctgctaat tgtttttcgt
      421 ctaaaggatg ttgagaatgg cgcacgatat caactaatgc tttttctgct ctcgcgtagg
      481 taaattctgg ggatgattga acaatctcac ctaataattc actggcacga tccaatgatt
      541 tatcatcacc atgcagtaaa taatcatgtg cctgataaaa attagttaat aacgcaccac
      601 gatgcggcaa aatttgctgg agcatctcct gcattcgttg tggccacggt tggtttaacg
      661 cttttgataa actctggagc aaatcatttt gaatcgccag ctgattaccg ttagtgatga
      721 cataacgttt atccagcatg gttgaaccat ctgcattgtc taccaatttt accgacataa
      781 agcattgttg agcacggtat tggcgctgat taacaaacgc aatagataat gttttaccgg
      841 aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg caccataaag gtggagaagg
      901 tgttaagtga tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc tgatacggag
      961 aactccagct gttacaactc ttattaacca tattaatgtc aatatcgcgt ggattgagca
     1021 aaatgcgcga tttactcata ggaagacgtg tttcaagact tgaaaacgct accagtgcga
     1081 cacagatacc taacgacaac aggaaaaaaa accataccca aaaggtagtg aatcgtttgc
     1141 ttttaactgg ggattgttca ggtggcgttg tggtgttttg aatgttaaga ctgtgggagg
     1201 gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct
     1261 ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta
     1321 cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg
     1381 agatactctg cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa
     1441 gttcatctct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga agatcgatta
     1501 atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa
     1561 caagccattc gccaacgcga actacaggtt gttgcataat agaaactcat tcgaaaaggg
     1621 a
//
LOCUS       DACADA010000345         1586 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.353, whole genome shotgun sequence.
ACCESSION   DACADA010000345 DACADA010000000
VERSION     DACADA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.353"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            154..438
                     /locus_tag="ISB17_004879"
     CDS             154..438
                     /locus_tag="ISB17_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAP1982702.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            442..777
                     /locus_tag="ISB17_004880"
     CDS             442..777
                     /locus_tag="ISB17_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAP1982703.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(836..1435)
                     /locus_tag="ISB17_004881"
                     /pseudo
     CDS             complement(836..1435)
                     /locus_tag="ISB17_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          410 a          351 c          399 g          426 t
ORIGIN      
        1 atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg
       61 tgtgtgcaac ttacttaata ccggacaact aagttgaaga aaaaaatact cgccttcggg
      121 cttatctcag cattgttctg ctccacgcca gcaatggcgg atatgaatcg caccacgaaa
      181 ggtgcattgc taggtgcagg cgttggttta cttactggca atggtgttaa tggcgtactg
      241 aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg tgacggtaaa
      301 aatgcgcgta aaggtgcaaa agtgggtgcc gccgttggtg ctgtcactgg cgttctgacc
      361 ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt
      421 ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggtctgttt
      481 gttttcgcac caatgacctg ggcgcaggac tacaatatta agaacggttt gccatcagaa
      541 acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca
      601 gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa
      661 tctccggaac tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagatccg
      721 caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat
      781 ttcatatgtt accgcgtcga tgaattcgac gcggtttttt agctaacggg cgggatcaga
      841 acttatattt caggcccacc atcattgcgg tgtcattgta gccattatca cccagctgca
      901 cgcccacatt accccagacg ctggccgccg gattcagatt gccatttacg ccatgtttca
      961 tttcccccag attacgagcc ccgtcttgtt cgactttcac accgttcatt gagacagcaa
     1021 aatctttact gttatggatc ccgttcattt caacaaaagg ttcaaagtcg tcaccgtgcg
     1081 gaccgctgtt tttctcctga gtacgaataa aggtgcggaa accaagtcgg ctctggatgt
     1141 tgtaatttgc gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga
     1201 cgcccatcca gatggcctgt gccgaggtcg ttctcaactg tccgttgctg tcacgccagg
     1261 cattatgccc gccaatttga catagccaaa gttggcttga acgctcctgt tcagttacag
     1321 gatcgatata gcgcgtttca cccgcacgat cgttcagacg cgtaacaaaa agagtgttag
     1381 cggctgccag gttggcgacg taactgccag cgtcgggtcg caggacgggg gtaggcgttt
     1441 ctgttgattc tgaagtgaca ttggaaaaaa atcactctct accattgtat atttttgcaa
     1501 tgggggcttt agtgccattt gttgtaatag aactttttta tatcaatgtt accttgtcga
     1561 atagcaactg cataacttcc atcagc
//
LOCUS       DACADA010000346         1583 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.354, whole genome shotgun sequence.
ACCESSION   DACADA010000346 DACADA010000000
VERSION     DACADA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.354"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..607
                     /locus_tag="ISB17_004882"
     CDS             <1..607
                     /locus_tag="ISB17_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HAP1982704.1"
                     /translation="FIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQ
                     WVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQY
                     KIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITKVNNETVSYF
                     CEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            607..1530
                     /locus_tag="ISB17_004883"
     CDS             607..1530
                     /locus_tag="ISB17_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP1982705.1"
                     /translation="MTLPHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRY
                     ADNRTKLPQANQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGS
                     IKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYNSRTPVNLATRMALDAF
                     NVALAKDLRINIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEV
                     MVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQL
                     LKQMTVENVNTAVKQYLSHPVNTYTGVLLPK"
BASE COUNT          477 a          343 c          341 g          422 t
ORIGIN      
        1 atttatcgaa ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa
       61 cacattccgt atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac
      121 catttttatg caatgggtta aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag
      181 ctttgtcagc cgttttttgg ggaatgggca taccagttgt attttccagg agtcctgcaa
      241 ggataatctg gttgttgaaa gtaatggaga catttacgaa tgcgaccatt ttgtctatcc
      301 acagtacaaa attggaaaca ttaataaatc ggaactcaaa acgatgaaca gtgtacaact
      361 gacagcgcaa aaaaaacgga tttcagcgaa atgtcagcaa tgtgtatata aacctatctg
      421 caatggtggt tgccctaagc atcgtattac taaagtaaac aatgagactg tttcctattt
      481 ttgcgaaggt tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtgga
      541 gttagctaag aacagagtac cgctttacca cattatggat gttgcaaaac aaatggagaa
      601 taattaataa cgctccccca cagcacgatt aacgacaata tttgggcatc gctacaaaat
      661 gctcaaattc aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagcag
      721 atgtatgaga cgcgctatgc tgataaccgc acgaaattac cgcaagcaaa tcagattgca
      781 cagtttactg ccgcagatgc actggctgcc gatcgccagt tgttttcatc tccagcggat
      841 atcacgtttg tcattgtcgg taatgtcgca gaagacaaac tcgtggcgtt aattacgcgt
      901 tacttaggat caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc
      961 gcgacggaca acgcatcggt tactgtaaaa gaacaaaatg aaccagtggc acaggtttca
     1021 cagtggaagc gttataattc ccggactcct gttaatctgg cgacgcgtat ggcgctcgat
     1081 gcttttaacg tcgcactggc aaaagatcta cgcattaata ttcgtgaaca ggcatccggg
     1141 gcatacagcg tttcttctcg cctctcggtt gatcctcagg ccaaagatat cagtcatttg
     1201 ctggctttta cttgtcaacc agaacgacat gatgaactgt taacgttagc gaatgaagtg
     1261 atggttaagc gtctggccaa agggatcagt gagcaagaac tgaacgaata ccagcaaaac
     1321 gttcagcgca gcctcgatat ccaacagcgt agcgttcaac aattagcgaa caccattgta
     1381 aatagtctta ttcaatatga cgatcctgca gcatggactg agcaggagca attgttgaaa
     1441 caaatgacgg tagagaatgt taacactgct gttaaacaat atctttctca tccggtgaat
     1501 acctataccg gagtattatt gccaaaataa tcacagcccc gtcaacacat cgttggcggg
     1561 gatttcatca atattcgcta ttt
//
LOCUS       DACADA010000347         1577 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.355, whole genome shotgun sequence.
ACCESSION   DACADA010000347 DACADA010000000
VERSION     DACADA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.355"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..830
                     /locus_tag="ISB17_004884"
                     /pseudo
     CDS             1..830
                     /locus_tag="ISB17_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            1262..1417
                     /locus_tag="ISB17_004885"
     CDS             1262..1417
                     /locus_tag="ISB17_004885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982706.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAAGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
BASE COUNT          514 a          347 c          321 g          395 t
ORIGIN      
        1 attaaaagaa ataaaaccag aaacacatac tctcgtgttt gcaggaggta ccgcattatc
       61 aaaagcaggt atctcgctaa acagaatgtc agaagatatt gatataaagc tcgtgccaac
      121 agagaacttt atgcaaaatg gcagagacaa gagaagaaaa attcgcaagg aaatcgtgca
      181 aataatcacg gatgttattc ataattctga cattttcagc ttggataatg aaaacgcccg
      241 tataacacgt gatgagtatc gttataacga aatctcggta cgttatcccc agacatttgc
      301 acaagtcccc tgtcttcgtc catttattaa gctggagttg atggaaagca ctctgctgga
      361 acatccggaa agcagagata tttattccct tgtcacggag ttaacgggta aaggtacacc
      421 tgtaacagca ttcccctgtg ccaccatttt atccactcag gctgaaaagc tcatctccat
      481 gatgcgaaga acagcagcac atcttcgtaa tcccgaacaa caggatgatg agtttctggt
      541 acgtcacatt tacgacaact actgcattgt cagggaaaaa ggtgtgaatg ttcctgtatt
      601 gaaaaatttt gttcagatat gtattcagct atggtaacca gtacccacaa ttttgtagct
      661 caccagttga tgaacttaag aaagggctgg atgcactccg ggattatcct gtacacaaat
      721 tgcgctttca acgatttgtt aaaccgatgg tatttggtaa tacacagatt aactgggaag
      781 aagcctattc cagtttcaga caaacagcgc taagtgttct gaccagctaa caaacgcgtt
      841 aagtacgatt ccacaattca gcatatcgaa ccagaccaaa tgattacagc aaagcacaaa
      901 aaagagggat tatagcgtca aaagaattcg aattgcacat gggggcctca ttggtggcct
      961 caaaaacaac aaatacaaaa taatctatat aaaacaattt gttatacaat cagtttcatt
     1021 ccgattccgg gcaccactaa ttcttaagaa cccgcccaca aggcgggttt ttgcttttgg
     1081 atctttatat aaaataaact tgggagcata acgccatcag gtcttaaaca ctcccgaatc
     1141 atcaataaac atcccccgct aacaaaacac agttcagttc acataacatc cctgttatcc
     1201 cacacatatc tgctggccga acgacgccag tgcgcgtacg gaggcaagag cagccctgca
     1261 catggcagcg cttgtggtaa cctggtttaa tccggtgaaa gaggcgtttt acatgcatct
     1321 gccagcagcc ggaaaaacca aaaaagtggc gctggttgac tgtatgcgta aattgctgac
     1381 catcctgaac gcaatgctca gaaagaacga agagtgaaat gaatcgtagc atcatgttgc
     1441 tccataattt ttacgttcaa gacagttgat tatcggcgca tatgactcaa aaagagcctg
     1501 atgattgcct cttttccata ggctctttct ctgaagtgaa ggaagacccg gaagaaagca
     1561 ctcgctggca tacgtga
//
LOCUS       DACADA010000348         1512 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.356, whole genome shotgun sequence.
ACCESSION   DACADA010000348 DACADA010000000
VERSION     DACADA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.356"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..424
                     /locus_tag="ISB17_004886"
     CDS             <1..424
                     /locus_tag="ISB17_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP1982707.1"
                     /translation="QRVSLGRNDLLIRTFPKGAFRLKGEQIDGSFLLNNETYLVEAKW
                     HSTKTGNADLHAFHGKLDQKISWARGVFISWAGFTKSGLDAWGRGKKVICVSGYDLVL
                     MLKNNISFRMLMEEKIRRAAETGNLYIKIDEIYPNISK"
     gene            552..1109
                     /locus_tag="ISB17_004887"
     CDS             552..1109
                     /locus_tag="ISB17_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982708.1"
                     /translation="MAKYHIESVQEWAPFTHNGQSYSLSHLNAHEITYKGKMQDFKFV
                     VTYGMHCFTKDGTPYNIPFKYQDARESISVCLERYEASKQLQHILPNLPSLMLYQTTE
                     EKYFTLQMMNSATNQLEPYKICVAFFKENRLMRIHVLSAFFARTGPGAPGEPIPQKPV
                     SLFKVAVDTAKKPRNSGRPKEVNNR"
BASE COUNT          462 a          306 c          349 g          395 t
ORIGIN      
        1 tcaacgagtc agcttgggaa gaaatgacct gcttattcgc actttcccta aaggcgcatt
       61 caggttgaag ggcgaacaga ttgacggcag ttttctgtta aataatgaaa cctatcttgt
      121 ggaagcaaag tggcatagca ctaaaacagg aaacgctgat ctccatgcat ttcatggcaa
      181 gctggatcag aaaatttcat gggctcgcgg cgtatttatc agttgggctg gatttacaaa
      241 aagtggtctg gatgcatggg ggaggggaaa gaaggtcatc tgtgtcagcg gttatgatct
      301 tgttctcatg ttaaaaaata acatcagttt cagaatgttg atggaagaaa aaatcagacg
      361 ggcagccgaa actggcaact tatatatcaa gatagacgaa atttatccaa acataagcaa
      421 atgatgcttc ctgtcctgtc tacaatcagc gtaactggtg ggattacagc tccacacctt
      481 gcagcattag cacttcagct tgagcggata aaaactatgg tagaattcaa aaaaaatagc
      541 tggggatgac agtggctaag taccatattg aatctgtgca ggaatgggcg ccgtttactc
      601 acaacggcca gagttactct ttgtcacatt tgaatgcaca tgagattacc tacaaaggta
      661 agatgcaaga ttttaagttt gtcgtaactt atggcatgca ctgcttcact aaagacggta
      721 cgccttacaa catccccttt aaatatcaag atgcgagaga gtcgatctcc gtttgtcttg
      781 aacggtatga agcatcaaag caactgcaac acattctgcc caaccttcca tcgttgatgc
      841 tctatcagac cactgaagaa aagtatttca cgcttcagat gatgaatagt gctacgaacc
      901 agcttgagcc atacaagatc tgcgttgcat tcttcaaaga gaaccgtttg atgcgtatac
      961 acgttctttc ggcatttttt gctcgtactg gacctggtgc accgggcgag cctatcccgc
     1021 aaaagccagt tagcttattt aaggttgctg tggatacggc aaaaaagcca agaaacagtg
     1081 gacgaccgaa ggaagttaac aacaggtgag aaagtggcgg ccccctttcg ggggcctcag
     1141 ccatttagtt tgattagata atcaagacta aacagggcag gtcgcatttg cttcgaactg
     1201 gtcgaaccgt ttccggctgc gggatgcgat tcattgctga acccctgtaa ccatccgtta
     1261 ttagtattct attatctgac gtagataagt caagtaatgt agttcttact gccgcaagga
     1321 ctttctgaag aagatagtac atcttgtcgg aagttaggag taattacttg agtaagaaga
     1381 tgtagttcta tcaggtacgc acagtgcaca caaagaaaca agttatccgc tgtgaagatg
     1441 ttcaagatag acgagcaaga ttagcttaaa aaaacagcct tagcgtaaaa taattatcga
     1501 tatccaagct gc
//
LOCUS       DACADA010000349         1474 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.357, whole genome shotgun sequence.
ACCESSION   DACADA010000349 DACADA010000000
VERSION     DACADA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.357"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            535..779
                     /locus_tag="ISB17_004888"
                     /pseudo
     CDS             535..779
                     /locus_tag="ISB17_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(796..1467)
                     /locus_tag="ISB17_004889"
     CDS             complement(796..1467)
                     /locus_tag="ISB17_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAP1982709.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
BASE COUNT          438 a          332 c          330 g          374 t
ORIGIN      
        1 gtggtaaacc tatagcgtaa attcagattg ttaaattaac tctactactt tacacgttag
       61 ttatgagcga tttctgatag tgtctggttt aatcagagct ttattatctg cgacgtttat
      121 ttttatttaa gagagccacc tgcaagttgt tattccacca gagagtgaat cgcaaagcag
      181 gtcatcattt catctgtata tctgtatgcc aaatggcatt ccccctcctt tttcgccgat
      241 attttgttgt gatcgacgaa gtttcgctta aaaaagcacc agcagtggtt tcgcagctat
      301 atggtgtata aaaaatgttc tcatacagat gttttgtgaa tgtgtgggtt gacattcata
      361 tgaaaaaaat cataattcca tcatgtttgt gtgggggctt ttctgtatct tacgcatcgc
      421 actcaagcct gacagaaaat atgctgtaag gctcatatca aaaaccgcca ttagctcatc
      481 aggaagagca gacgacaacc aggattgttg tatggtacgg ggttcgaggc ctcgatggcg
      541 gtccaacttt tcaaaactct tctgaatcag atccccttat tatcatccct gcaaagtgga
      601 acattgccat tgtttggcta ttcaggacgg gtcagaccaa tgaaaaagcc cacacagggg
      661 agagtaggct gaaatgggaa gctaaagact caagtaaact tatcggaaat aaggaccacg
      721 cattacgggg gctatcatcg ccgatggttg ggattcggca aatcaggttg attacgtaga
      781 gagtaaatta tctgctcacc gctgcgtcac cccttcgtaa atcaggcgta gcgcgaatac
      841 accaataatt gcaccaataa cccgactggc aacgcgttgc atacgcccat aagcacgacg
      901 cacagcgggc aaagaaaacg cctggcttag aaagactcgc cagataattg atgtgagcac
      961 aatccccgcc caggccatta atcgtgccca tgttggtgtt tcggcattta atgttactga
     1021 gaaaatactg ataaaaaata aaacggtttg cgggttagag agatcggtaa ttaatccacg
     1081 gcggaaaaag atataccagg gggcactaat cggttgttgt agtgtgctca tttgcggtgt
     1141 tgactggcgg cgcatgctgc accacgcaaa ccataagaga taagcgccgc cgacgattct
     1201 gataagcgaa aaaatctcct cacactgcgt aattagcgtt gcaagaccaa acaaacccaa
     1261 cccggaataa aatgcatcgc ccagcgccac gcccagcccg gtcaacaccc ccgcgcgtcg
     1321 accggaagcc aggctggttt gtactaccac aaagagattg gctcccggat taaaaaaagt
     1381 aatcacgaac aatcctacgg tcaggtaaac ggcgtgcaaa ggatccatag tgatttcatc
     1441 cataaataag tgaagtaact tcatcatatt tcta
//
LOCUS       DACADA010000350         1457 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.358, whole genome shotgun sequence.
ACCESSION   DACADA010000350 DACADA010000000
VERSION     DACADA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.358"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..471)
                     /locus_tag="ISB17_004890"
     CDS             complement(<1..471)
                     /locus_tag="ISB17_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HAP1982710.1"
                     /translation="MRKGKIIVASMMVLCVLIFVAAAAWFMLFQGEMTEPTEEEKQAA
                     ILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVAGSSADTARQKCEKLAPER
                     DPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMMHAFLALGLDGRTVRN"
     gene            complement(708..1214)
                     /locus_tag="ISB17_004891"
     CDS             complement(708..1214)
                     /locus_tag="ISB17_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HAP1982711.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            complement(1204..1431)
                     /locus_tag="ISB17_004892"
     CDS             complement(1204..1431)
                     /locus_tag="ISB17_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scsC protein"
                     /protein_id="HAP1982712.1"
                     /translation="MTCRGSPYSTIILYIQTDPDEKSMETLSTNLQLARLVGVQGTPA
                     TIIGDEMIPGAVSWETLEAVVKEKLAVAHAQ"
BASE COUNT          356 a          434 c          329 g          338 t
ORIGIN      
        1 atttctcacc gtacgaccgt ccagccccag agcaagaaac gcatgcatca tggatttcgc
       61 acccttaccg gtaactggaa tgataacctg ctgaatttca ttatttttat ctttcacaaa
      121 gaacacatcc gccaccgtac agcgctgacg aacctgcgct gggtcgcgtt caggggcaag
      181 tttttcacac ttctgccgcg cggtgtccgc gctgctgcca gccaccagtt cgccggaatc
      241 taaattaaca tggcgttgaa taatataacg atgatagagc gtttcgactg attttttatc
      301 ctgggtctct gatttcatca ggcccgcagc gtgtaaaatt gccgcctgtt tttcctcctc
      361 tgtcggctcc gtcatttctc cctggaacag cataaaccat gccgccgccg caacaaaaat
      421 aaggacacaa agcaccatca ttgatgcaac aataatttta cccttgcgca ttgaattgtt
      481 atccctgtca aaatggaaat cgaaaaaatc gcctgaacgt atcacagact ttcattcaat
      541 taaaacagat atgccattat caattcaggt ttgcaacgtc aggcagcggt aaaaaataat
      601 aactggcgtc ggtaaaaaag cagcaaaaat aacagaaaac acgctctccg ctcgttttac
      661 acatccttac aatgggttaa aaaatgccgg gtttccccgg cacgctatca aaacatggct
      721 gcccgccaca gacgtaactt catgccccag tagctggtcc agccgctggt cgtagttacc
      781 acctgcccct tcgacaccac caccagcgtt ggcgtgacgc tgatttccca gtttcgtgat
      841 atctcaccgc cgctatcatt cacgacgggg aacgtcaccc ttttacgcga taaccagcgg
      901 gaaacctccc cgtcattacc ggaacgcagc gcgatggtca tgacgctctc cccttccgac
      961 tgcaaacggg cgacatccgg cgtggtgaaa cggcagatac cgcaccagct cgcccagaag
     1021 tagagcagca ccgggcgttc ttcactgatc gacgccagcg ttacggtttc cccgtccagc
     1081 gtatgcagcg gcgtgctgtc aaacatggcc ggcatctgcg gagaacgcca gacatccagc
     1141 aggattatca cgccagccag cacaaccagt aagatcgcgc cttcacgcag ccagcggcgc
     1201 agtttactga gcatgggcca ccgccagttt ttctttcacc accgcctcaa gggtctccca
     1261 cgataccgcg ccggggatca tctcgtcgcc aatgatggtt gccggcgtcc cctgaacgcc
     1321 aaccagccgg gcgagttgca gattggtgct caacgtctcc atgcttttct catcggggtc
     1381 agtttggata tagagaatta ttgtactata agggcttccc cggcatgtca atgccggtgt
     1441 ttacggtttc tgtctgc
//
LOCUS       DACADA010000351         1455 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.359, whole genome shotgun sequence.
ACCESSION   DACADA010000351 DACADA010000000
VERSION     DACADA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.359"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            4..1410
                     /gene="gndA"
                     /locus_tag="ISB17_004893"
     CDS             4..1410
                     /gene="gndA"
                     /locus_tag="ISB17_004893"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP1982713.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQRAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          366 a          354 c          377 g          358 t
ORIGIN      
        1 acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag tgatggggcg caaccttgcg
       61 ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgttcccg tgaaaaaacg
      121 gaagaagtga ttgccgaaaa tccaggcaaa aaactggttc cttactatac ggtgaaagag
      181 tttgttgaat ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc
      241 acggatgctg ctattgattc ccttaagccg tacctcgata aaggtgacat catcattgat
      301 ggtggtaaca ccttcttcca ggacactatt cgtcgtaatc gtgagctttc tgcagaaggc
      361 tttaacttca tcggtactgg tgtttctggc ggtgaagaag gcgcgctgaa aggaccttcc
      421 attatgcctg gtggccagaa agaagcctat gaactggttg cgccgatcct gaccaaaatc
      481 gccgcagtgg ctgaagacgg ggagccatgc gttacctata ttggtgccga tggcgcaggc
      541 cactatgtga agatggttca caacggtatt gaatacggtg atatgcagct gattgctgaa
      601 gcctattctc tgcttaaagg tggcctgaac ctcaccaacg aagaactggc gcagaccttt
      661 accgagtgga ataacggtga actgagcagc tacctgattg acattaccaa agatatcttc
      721 accaaaaaag atgaagacgg taactacctg gttgatgtga ttctggatga agctgcaaac
      781 aaaggtacgg gtaaatggac cagccagagc gcgctggatc tcggcgaacc gctgtcgctg
      841 attaccgagt ctgtgtttgc acgttatatc tcttctctga aagatcagcg cgttgccgcg
      901 tctaaagttc tctctggccc gcaagcgcag cgagcaggcg acaaagctga gtttatcgag
      961 aaggttcgtc gtgcgctgta tctgggcaaa atcgtttctt acgctcaggg cttctctcag
     1021 ttgcgtgctg cgtctgaaga atacaactgg gatctgaact acggcgaaat cgcgaagatt
     1081 ttccgtgctg gctgcatcat ccgtgcgcag ttcctgcaga aaatcaccga tgcatacgcc
     1141 gaaaatccgc agatcgctaa cctgctgctg gctccgtact ttaagcaaat cgccgatgac
     1201 tatcagcagg cgctgcgcga tgtcgttgct tatgcagtac agaacggtat cccggttccg
     1261 accttcgccg ctgcggttgc ctattatgac agctaccgtg ccgctgttct gcctgcaaac
     1321 ctgatccagg cacagcgtga ctatttcggt gcacatactt ataagcgcat cgataaagaa
     1381 ggtgtgttcc ataccgaatg gctggattaa tctgacttaa ctaacaataa attgaggccc
     1441 ggcgtatatt gtgcc
//
LOCUS       DACADA010000352         1447 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.360, whole genome shotgun sequence.
ACCESSION   DACADA010000352 DACADA010000000
VERSION     DACADA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.360"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..699
                     /locus_tag="ISB17_004894"
     CDS             <1..699
                     /locus_tag="ISB17_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982714.1"
                     /translation="IKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQK
                     DIEILLEKSSQPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVD
                     GQPRQSHGKGVRAILYSAFIIGLLKYCIKKGYPHPGLVVIDSPLTSYKKKAAATVSGF
                     DEPISAGIEEGFWHSLTQLPDTLQIIVIENKEPPVSIMNKVHYEWFAGEHAMDGERRA
                     LIPLPSKNSAILMK"
BASE COUNT          412 a          285 c          360 g          390 t
ORIGIN      
        1 atcaaatata cttttgagcc tcttcttaca ctttccaaag aaagcctaga tttagctatt
       61 gagcagcgag ctgaaagaga ctctgagttg aatgatcgac agagatggtt tgagttacta
      121 aaacatcaaa aggatataga aatactgctg gaaaagagca gtcaaccacg gctcgaatgg
      181 gagggattat ctacccgtac gctggccgaa ctttgtagag aaattgaaac tgttttaaaa
      241 gactggaaat ggggtgccga gcctgacgtt tcatttaatg aaaaagagta tgacatcatt
      301 gttgatggac aacctcggca gtctcacggt aaaggtgttc gtgccattct ttattcagct
      361 ttcatcattg gattactaaa atattgtatt aaaaaaggat acccgcatcc tgggctagtc
      421 gtgattgatt caccgctgac aagttataag aagaaagccg ctgctacggt atcgggcttc
      481 gatgaaccca tatcggctgg tattgaggaa ggcttctggc attcactgac ccaattgcct
      541 gataccttac aaatcatagt tatagagaat aaagaaccgc cggtatcaat catgaataaa
      601 gttcattacg aatggtttgc gggtgaacat gcaatggatg gagagcgcag agcccttatc
      661 ccgctgccct caaaaaatag tgctatttta atgaagtaac ttaggatggg tagccatcaa
      721 atcgcagtta tacgattggt ggggtttgta aaagcggtag tcttgattac cactgcattc
      781 attctacaag cacatacttc cctaggttgc tgtagttatc cgattacacg ccttttctga
      841 aagcagcctc agggagccgg ataaaacccg ttaaaagtgg cttgcctata ctgctgcgcg
      901 gcgctgacgc tttagaggcc ccaggattta ggcaaatcaa tgtagttagc tcagtagcag
      961 gtcatatctg ggctataaat ggcattacgt gacactgaaa gagcttccca gtggcatcgt
     1021 atttggtgag ccccaaccta caagacgggc ggggatagcc gtgattggca cccacagcaa
     1081 ggcgataaag atgttcaacg gagcggctca gcaggtatgg ggatggtagg atttagtctt
     1141 catatgcctg tggaattaat caaccctcag agcccgacac acccggcatt gcttacggta
     1201 taagactgat tgagaaggtg tttgacgttt ttgtatccca gtctgtcggt aaagtaaaca
     1261 cagtgatcag taactggtag tactggaagt gattgcggtc gtaatgctga ttttgcacgc
     1321 tacgacaggt atattctgtt tggtatctgt agaggttgtg atggcgctga ccttgcacat
     1381 gccaggtacg ttagacttaa taataaaggg agtattagca ggagggattt tttcggactc
     1441 agttgta
//
LOCUS       DACADA010000353         1428 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.361, whole genome shotgun sequence.
ACCESSION   DACADA010000353 DACADA010000000
VERSION     DACADA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.361"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(103..996)
                     /locus_tag="ISB17_004895"
                     /pseudo
     CDS             complement(103..996)
                     /locus_tag="ISB17_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          453 a          313 c          282 g          380 t
ORIGIN      
        1 caaacaatta tagcaaacaa aagaatgaac aatagtaaca agaggcaacg agctaattag
       61 ctgtctataa ttacaccgta ccatggcctt actgatcaat tttcactcaa ccacaacctt
      121 caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag gtttatagaa
      181 ataataaccc tgctgaaatg taatattatt ttggttcaga tagtcaagtt gttctttcgt
      241 ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca atacacaatc
      301 cagaattcgg gttgattctg ggtctgcgtt gacgcggcca acgaaactgt gatcaatttt
      361 tatataatca atatgcaggt catgcagata agaaagccct gagtaaccag taccgaaatc
      421 atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa ccagactttc
      481 atcaacattt aatggttcac gctcggtgac ttccagaaca aggtttaaat cgcggcgggt
      541 aaaactgtca cggtaattta agcactcgtc gacaaacgtc ggcgaaacaa tatgcgaggc
      601 gctaaaatta atcccaatat gaaaaccttc cggcagttta ctcgcgatag cgttcatctg
      661 ggtggcaacc tgattcatca ggctttgcgt aagcggaacg attaatccgc atttttcagc
      721 aagtggaata aatgccgcag gtgatatata tccaccgtga ggttgtttcc agcgggctaa
      781 cacctcaact ccccgcaatg tcccttcccg accatttacc acaggttggt aaaaagggac
      841 gatctcccca ttatttatag cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa
      901 atacctatca agaaggaagg cagcggcgca ggcgattaac agaataaata tcaaaatccc
      961 aaagccttta tcgataagtc tgtagaagct aaagagacgt tcattcgctc gcgctggggt
     1021 ctacagatag catggtagag agcatcacaa atatccacgc cgaagataaa attttctcca
     1081 acttcatcta acctattaat tatttagaac aatgtaggca gaattaaaca aattaggcgg
     1141 gatatcaggc gtcaagaatg gaaaacgagc gctctccatt cctgtttgcc ctctgatatt
     1201 gcggatatat taagaaagca taacgcctga aatgctcact ttgcatcagc atagtgatac
     1261 agctgatgtt tattcaaaaa ccttactcaa gttttaagag agcacggatt ccctgtcatt
     1321 aataatgata tccgttagtg tagtcttatg catttcaatg tactgcaaga tttgcagata
     1381 gagatcagaa agaataacac tggtctcatc ttttaaacgc aaccgtaa
//
LOCUS       DACADA010000354         1409 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.362, whole genome shotgun sequence.
ACCESSION   DACADA010000354 DACADA010000000
VERSION     DACADA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.362"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            37..>1409
                     /locus_tag="ISB17_004896"
     CDS             37..>1409
                     /locus_tag="ISB17_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982715.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQIYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNTHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVTDSGVSTADSSFT
                     LDGDSSPT"
BASE COUNT          396 a          261 c          331 g          421 t
ORIGIN      
        1 tatttttttc attatattca taacgttgat tgaactatga ccactgtgaa taaaaagtta
       61 aaaaaaactg catctggcgc gattacatgg tcggtaattg ttacccagat attatctcca
      121 gtttctcttt ctttgatccc agcaaacagt tttgcatcat ctggtaataa agatgttacg
      181 caaatttatg ccagcgatga gcatgcaaat aaagtggcct catttgcagc aagtgcaggt
      241 cagagcctgg cgaataataa tgcaagtagt tttgctgtaa atactttatc aactcaggcg
      301 acaaaagagg tcgtcgactg gttgcaacaa tatggcaatg cgcgcatcaa gcttaatgtc
      361 gatgattctt tttccttgaa ggattcatca ttcgactttt tatatccatg gctggatact
      421 caggattatg tgttattcag tcagacatca ctacatcgta ctgatgaccg aaatcagacc
      481 aatattggtt tggggattcg tcatttcact cctgataacg caatgttggg tgcgaatgtt
      541 ttctatgatt acgatttaag tcgtagtcac tctcgtgcag gttttggggt tgagtactgg
      601 agggattatt tcaggcttgg tgtaaatacc tattttggtc tatctgactg gaagaacagt
      661 cgtgatattg atgattatct ggaaagacct gcaaatggct gggatttttc tgctgaagga
      721 tggctacctg cttatccgca attaggggca tctattcagt ttgaaaaata ttatggtaaa
      781 aatgtcgggt tatttggaag cgataacctg caggaaaacc cttacgcagt tactggggga
      841 atttcttata caccagttcc tctgattaag ttttctgcac agcataagca aggacagagc
      901 aacactcacg atacaacctt tggtgttgag tttaattatc gcccgggcgt ttccttggct
      961 gaacagctta gcagcgataa tgttgcagtt atgcgagaag tccagaaccg gcgttatgat
     1021 tttgttgaac gaaataacaa cattgttctg gagtacaaga agaagcatgc actgaaaatc
     1081 agtttgccag agtctgttca gggggagggg gaatcaatca tccctgtaac actgacaatc
     1141 aacaatgcta gtggtggtat caagtctgta cagtggaatg atagtgcatt cactgcggct
     1201 ggcggcaaga tcagtggaaa tggcacatca tggcagatca ctttaccggc ttataaaagc
     1261 gaaggtgtaa attcgtggaa tgttggagcc acagtacagg ataacagagg caacgtttcc
     1321 aactatgccg tgatgaatat tagcgttact gatagtggtg tctcgacggc tgattcgtct
     1381 tttacactgg atggagattc gtcgccgac
//
LOCUS       DACADA010000355         1406 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.363, whole genome shotgun sequence.
ACCESSION   DACADA010000355 DACADA010000000
VERSION     DACADA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.363"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            61..>1406
                     /locus_tag="ISB17_004897"
     CDS             61..>1406
                     /locus_tag="ISB17_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982716.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSV"
BASE COUNT          409 a          292 c          306 g          399 t
ORIGIN      
        1 gttttctatg cattgataat tgatggatca acttattacg tccctgagga gggatgacaa
       61 atgcactcct ggaaaaagaa acttgtagta tcacaattag cattggcttg cactctggct
      121 atcacctctc aggctaatgc agcaaactat gatacctggg ggtatcatga caacaccacg
      181 accgatttag tttggccgta tgacactgac aatgatggaa ttgccgatgc tggcggcatg
      241 gacccgatgg gttcagacgg taactacgta acttacaatg gttttgttta ctataacaat
      301 gccaatggtg atttcgacac cgtctttaaa ggcgatactg ttaacggtac catttctact
      361 tattacttga atcatgatta cgctagcggt tctgttaacc agttagatat cagcaattca
      421 gtaattcatg ggtcaatcac ttctgaactg ccatttggtt attacagcat tactcctgca
      481 gaagttaatg ctaaaggtga agttgtaact ccacagacct atagtggcta taatttctat
      541 aacggaatca ctactgacct tgactacaat tggtacgatg gtgacgtatt cactctgaac
      601 gtatccaatt ccaccattga tgatgactat gaagctcttt acttcactga cacatatctt
      661 gatggtgata aatctaagtc aaccaatgaa acgttctata ccgctctggg tgttgcagta
      721 aatcttgatg ttgaaagcaa tatcaatatt actaacaact cccgtgtagc tggtattgcg
      781 ctgtctgaag gcaatacaag caacacgact tatactagcg aatatcatca gtgggacaac
      841 aacattaatg tgtctaactc cactgtaact tcagggtcac agactccgct ggaagaaagc
      901 aatggcttct tcggtaaatc tgctgaacca agtgattacg ctggtaatgg tggcgaaaat
      961 gacgtagctc tgtcattctc tgacaatgct ggctcaaatt attccatgaa aaacaatgta
     1021 aactttgacc attcaaccct gttgggtgat gtcgagttca caagccactg gaataacgat
     1081 ggcgtattct tctattcaac cggccatgac agcaatggtg acggtgtcct tgataccaac
     1141 ggtggttggg tagatgatgc tcagaacgtt gatgaactga acattactct gaacaacggt
     1201 agtaagtggg ttggctcagc aaacatgagt gctgaagtaa ttgctccggc agatatgtat
     1261 gatgttgcgc cgaacagcct gacccctggt gccactatcg aagctaacga ttggggtcgt
     1321 attattgaca acaaggtatt ccagagcggc gtatttaacg tagcactgaa caatggttca
     1381 gaatggaata ccgttaatag ttcagt
//
LOCUS       DACADA010000356         1394 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.364, whole genome shotgun sequence.
ACCESSION   DACADA010000356 DACADA010000000
VERSION     DACADA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.364"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            73..>1394
                     /locus_tag="ISB17_004898"
     CDS             73..>1394
                     /locus_tag="ISB17_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP1982717.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
BASE COUNT          334 a          340 c          390 g          330 t
ORIGIN      
        1 atggttttgt gttttttctg tattacatgt caggtatttt aaagaatcat ttttcagatg
       61 gtggaaagaa ccatggcatt taaacactat gatgttgtca gggcggcgtc gccgtcagat
      121 cttgcggaaa agctgacaca taaactgaaa gagggctggc agccgtttgg tagtccggtg
      181 gccataaccc cttataccct gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt
      241 ggtgcaactg agccggagtg gtactacgtc atcgtactgg ccgggcaatc caatgccatg
      301 gcttacggtg aagggcttcc gcttccggat tcatacgatg cgccccatcc gcgcattaag
      361 caactggccc gtcgtaacac agtgactccc ggtggtgaag tatgcgtatt taacgacatc
      421 attcctgctg accattgtct gcatgatgtt caggatatga gtacgattaa ccatccccgg
      481 gctgacctga gcaaagggca gtacggctgt gtcggacagg gcttacatat tgccaaaaaa
      541 ctgcttccgt atatccctaa taatgcgggg atcctgctgg taccatgctg tcgtggtggt
      601 tcggcattca cccagggcac ggaggggaca ttcagcgagt ccacgggggc cagtcaggat
      661 tcggctcgct ggggagtggg taagccgtta tatcaggatc tgcttttccg cacgaaggca
      721 gcattgcaga aaaacccgaa aaacgttttg ctggcgatat gctggatgca gggggaattc
      781 gatatgacga atgccagtta cgcccagcag ccagcagcat ttcttgcaat ggtacagcag
      841 ttccgtgctg accttgccgg gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc
      901 ccctggattt gtggcgacac gacatacgcg tggaaacaag aacacggtac gcaatatgaa
      961 gtggtatatg gtgcatataa aggtaaagaa tcccagcaga tttattttgt tccctttatg
     1021 accgatggta gcggagttaa tacaccgaca aacaacccgt cagaagatcc tgatattgcc
     1081 gggtctggtt attacggttc ggcatcccga acgaacaaaa actgggtatc atcaaatcgc
     1141 ccgacgcatt tcagctcatg ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct
     1201 attctgaacg tagccggtcg caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa
     1261 ccctcgcccg gcggcgacac gccatcgggg ccgtctgaag atgcatccgt acgcacaatc
     1321 tccctgttgc cgacagccgg agatgctgct gcgcagggct ggagcattaa gaatggcgga
     1381 attcagttgt caga
//
LOCUS       DACADA010000357         1386 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.365, whole genome shotgun sequence.
ACCESSION   DACADA010000357 DACADA010000000
VERSION     DACADA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.365"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(143..1186)
                     /locus_tag="ISB17_004899"
     CDS             complement(143..1186)
                     /locus_tag="ISB17_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982718.1"
                     /translation="MEKGTIFPAMGYLHIDGRGMKPNGGSRYNPLEAETIAAWLVAHK
                     DDIERHYGEPLYKVVGVVTPFSAQVNAIKTSLRKLEINGKDEQGSLTVGTVHSLQGAE
                     RAIVLFSPVYSKHEDGRFLDSNSSILNVAVSRAKDSFLVFGDMDLIEMQPAFSPRGLL
                     AKYLFSSDNNALQFEFQKRQDLISAHTQISTLHGVEQHDGFLNKTLAGAQKKITIISP
                     WLSWQKVEQTGFLASMALARSRGIDITVVTDKNCNIAHVDDDKRQEKQHLLNDAVEKL
                     NKMGIATKLVNRVHSKIVIEDEELLCVGSFNWFSAAREDKYQRYDTSLVYRGEGVKNE
                     IKAIYGSLDQRQL"
     gene            1312..>1386
                     /locus_tag="ISB17_004900"
     CDS             1312..>1386
                     /locus_tag="ISB17_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1982719.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTV"
BASE COUNT          397 a          341 c          264 g          384 t
ORIGIN      
        1 ggcgcattat gcaaaggaaa atgattaggt aaacaataag atttataatc aggaaaggcg
       61 actcgcaata aaacggaaag tcgccttaac attggtaaaa tatgcccttt actactcctg
      121 tatcatgaac ggctcatcac atttaaagct gacgttgatc cagactacca tatattgcct
      181 ttatttcatt ttttacgcct tcgccacggt acaccaaaga cgtatcgtat cgttgatact
      241 tatcttcccg tgcagcactg aaccagttaa aagatccgac acacagcagt tcttcatcct
      301 caatcacaat tttgctgtgg acacgattaa ccaatttcgt agcgataccc atcttattga
      361 gcttttcaac agcatcattc agcagatgtt gtttctcctg acgcttatca tcatcgacat
      421 gcgctatatt gcagttttta tccgtcacca cggtaatatc aatgccccgg gaacgggcca
      481 aagccattga tgccagaaat cccgtttgct ccactttttg ccaagataac caaggggaaa
      541 taatcgttat tttcttttga gctccggcaa gagtcttgtt caaaaatcca tcatgctgct
      601 ccacaccatg cagcgttgaa atttgtgtat gtgcggaaat taaatcctgt cgtttctgaa
      661 actcaaattg caacgcatta ttatctgaag aaaagagata ttttgccaat aaccctcgcg
      721 gcgaaaatgc gggctgcatc tcgataaggt ccatatcgcc aaataccaga aaactatctt
      781 tagcgcgtga gacagcaaca ttgaggatac tgctgttgct atcaagaaac ctgccatctt
      841 catgttttga gtagacagga gaaaagagaa caatcgccct ttccgccccc tgcaaggagt
      901 gcacagtccc cactgtcagt gaaccctgct cgtctttgcc attaatttcc agcttacgca
      961 atgacgtttt gatagcatta acctgcgccg aaaatggcgt cacaacacca acaactttgt
     1021 acagtggttc cccgtaatgg cgctctatat catccttatg tgccaccagc catgctgcta
     1081 ttgtttcagc ttcaagaggg ttataacggc ttcctccatt tggcttcatg cctctaccat
     1141 caatatgtaa atatcccatt gcggggaaaa tcgtcccttt ttccatccct cttttaggct
     1201 gcaacttacc gtgatagcaa agtgagttac agtagccgat gaagtggttt actgaatttg
     1261 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgccat aatgaaaaaa
     1321 agaaatttca gcgcagagtt taaacgcgaa tccgctcaac tggtcgttga ccagaattac
     1381 accgtg
//
LOCUS       DACADA010000358         1383 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.366, whole genome shotgun sequence.
ACCESSION   DACADA010000358 DACADA010000000
VERSION     DACADA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.366"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..338)
                     /locus_tag="ISB17_004901"
     CDS             complement(<1..338)
                     /locus_tag="ISB17_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAP1982720.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKR"
     gene            complement(559..1290)
                     /locus_tag="ISB17_004902"
     CDS             complement(559..1290)
                     /locus_tag="ISB17_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP1982721.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          361 a          319 c          305 g          398 t
ORIGIN      
        1 ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcctgcgcc tgccagtgtt
       61 gaacagtatt cccgaaatgt agattttccg cgaatatgaa gcaatgcggc ctcttttatt
      121 ttagctgggt tcttggtcgt actaactttt aacaggttcc tggttcctct taataacaaa
      181 accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      241 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      301 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      361 gtgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      421 ctgtgatatg tacaaagtgt caaaggggga ttacctgaat tctcccggtg agcattttgc
      481 aacggaccag ctccgctaca aacgctgttg tgggttcaga ttataacatt ctgtctaagg
      541 ggcgggataa aggtgaattt agggggcatg aaagatgact ttataacctt gctcacccca
      601 gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
      661 ttgcgttggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa cagaaatgtg
      721 ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct cttcaacatt
      781 ccacgccatc agaatggcct ctttaccggg cttacggcgc atttcgaaaa gtgaggccgc
      841 tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg tgttgtgatc
      901 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
      961 ctctcgaccc agtgaaataa ttcgcgcgcg tagtttggct ggattagcca tacgaagaat
     1021 tgacatcatc tcttcttgca ggcggctcca gtcatcttgc gtatcctggc gggtagtttc
     1081 cagtaatgct ttaactttgc ctacaggaac gccattactt atccaacgct tgatctcttc
     1141 gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gttgtggttt
     1201 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1261 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcata aatctccgtc
     1321 gcctctacac gacccaataa tactttgtac aatatacgct aaaattgtac aaagtataaa
     1381 taa
//
LOCUS       DACADA010000359         1377 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.367, whole genome shotgun sequence.
ACCESSION   DACADA010000359 DACADA010000000
VERSION     DACADA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.367"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..714
                     /locus_tag="ISB17_004903"
     CDS             <1..714
                     /locus_tag="ISB17_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1982722.1"
                     /translation="KSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHR
                     LHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRR
                     FHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVL
                     VDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVL
                     KPAVLERQRKSKGVPRGEA"
     gene            701..>1377
                     /locus_tag="ISB17_004904"
     CDS             701..>1377
                     /locus_tag="ISB17_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAP1982723.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKA"
BASE COUNT          252 a          441 c          481 g          203 t
ORIGIN      
        1 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
       61 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
      121 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
      181 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
      241 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
      301 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
      361 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
      421 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
      481 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
      541 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
      601 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
      661 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
      721 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg ggctgttccg
      781 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
      841 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
      901 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
      961 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
     1021 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
     1081 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
     1141 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
     1201 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
     1261 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
     1321 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggc
//
LOCUS       DACADA010000360         1351 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.368, whole genome shotgun sequence.
ACCESSION   DACADA010000360 DACADA010000000
VERSION     DACADA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.368"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            102..1313
                     /gene="bluF"
                     /locus_tag="ISB17_004905"
     CDS             102..1313
                     /gene="bluF"
                     /locus_tag="ISB17_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAP1982724.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          382 a          283 c          295 g          391 t
ORIGIN      
        1 caatgtaaag aaacggttat gttttggtat taaaagaaag acacaagaag atatgtctgt
       61 taccgtctta ctctcgcctc gcccattacc ctggattgtt aatgcttacc acccttattt
      121 atcgtagcca tatacgtgac gccgaacctg tcaaaaaaat cgaagaaatg gtttcgatag
      181 caaatcgcag gaacatgcgg tctgacgtaa cagggatctt actgtttaat ggttctcatt
      241 ttttccagct tctggaaggt ccggaagaac aggttcaaat gatatatcgg gccatatgcc
      301 aggatccacg gcactataat attgttgaac tgttgtgcga ttacgcgcct gctcgccgtt
      361 ttggcaaagc aggaatggaa ttatttgatt tacgcctgca cgagcgagat gacgttttac
      421 aggccgtatt cgacaaaggc acatcaaaat ttcagctaac ttatgatgac agagcgctac
      481 aattttttcg tacttttgtc cttgcaactg aacaatcaac ctatttcgag attcctgctg
      541 aagactcctg gctttttatc gctgacggat ctgataaaga acttgattct tgtacccttt
      601 catcaaatat aaacgaccac tttgcctttc atcctattgt cgacccttta tcgcggcgga
      661 taatcgcttt tgaagccatt gtgcaaaaaa atgaagacag cccgtcagct atagcggttg
      721 ggcagcgtaa agaaggggaa atctacacag cggatctcaa aagtaaggcg cttgcattcg
      781 cgatggcaca cgcacttaag ctcggtgata aaatgatttc aatcaatcta ttacctatga
      841 ccctggttaa cgagcctgac gcagtctctt ttttacttga tgaaataaag gccaatgctc
      901 tggtgcctga acaaatcatc gttgaattta ctgaaagtga agtcatatct cggtttgatg
      961 agtttgccga agcggttaaa tcgctaaagg ctgccggtat cagtgtagca attgatcatt
     1021 ttggcgcagg ttttgctggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa
     1081 tcagtcagga attgattacc aacgttcata aaagcggacc acggcaggca attattcagg
     1141 cgatcatgaa atgctgtaca tcacttgaaa ttcaagtctc tgctatgggc gtggcaacac
     1201 cagaagagtg gatgtggctt gaatctgcag gaattgagat gtttcaggga gatctgtttt
     1261 ccaaagctaa attgaatggt atcccttcag ttgcgtggcc ggagaaaaaa taattttcag
     1321 cacattattt cgcatgattt ctggaaatca t
//
LOCUS       DACADA010000361         1347 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.369, whole genome shotgun sequence.
ACCESSION   DACADA010000361 DACADA010000000
VERSION     DACADA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.369"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            151..>1347
                     /locus_tag="ISB17_004906"
     CDS             151..>1347
                     /locus_tag="ISB17_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP1982725.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANV"
BASE COUNT          371 a          292 c          305 g          379 t
ORIGIN      
        1 agaagcggtg acctggggct aacctgataa tcataataaa tgtaatgtat gccatttagg
       61 gctatgcccg cctgattcag tgctacgcat tgaatcaggt tcttttcgcg ttgaacaata
      121 tcaagatacg gctattcgca gggagtggat atgtttacca acgtaaatgt tgattgttgc
      181 aaaacaccag gatgtaaaaa cctggggttg ctgaatagcc aggattatgt cgcacagggt
      241 aaaaatattt tatgccgtga atgtggttac ttgtttccag tgatatctga acagtcgctt
      301 aatatttatc gtaatattgt gaatcactcc tggagaggtt tgatttgcca atgttcaact
      361 tgcggaggca cgtccctcaa aaaatatgga tattctgcac aaggccagag aagaatgtat
      421 tgccatcatt gtgagaaaac atttatcact ctggaacatg taattaccac accacgagga
      481 gcccagttag cattgatgat tgagcaaggg gaggcacttg cggatatcag aaagtcatta
      541 cttcttaaca gcactggact tagccgtgaa ctgttaaaat tagcgcgtga agcaaactat
      601 aaagaaagtc gacagtgttt cccagcttct gatattaccc tgagtacccg cgcttttcgc
      661 gtcaagtata atggtagcaa taactctctt tatgctcttg ttaccgcaga agaacaaagc
      721 ggcagggtgg ttgccatctc aaccaattac tccccatctg ccgtagagca acattatcaa
      781 tacacatcga actatgaaga gcgtatgtct ccagggacgc tggcacatca tgtccagcgc
      841 aaagagttac ttactatgcg gcgggatacc ttgtttgata ttgattacgg cccggcagtt
      901 ttacatcaaa acgatccggg aatgttggta aaaccggttc ttccggcata tcgtcatttt
      961 gaactggtca gaatactgac cgatgagcat tccaacaacg ttcagcatta ccttgatcac
     1021 gaatgcttta tattgggcgg ctgcctgatg gctaatttgc agcatattca tcaaggtcgc
     1081 tgccatattt cctttgtcaa agagcgcggt gtggcacccg ccaccattga ttttccgccg
     1141 cgattattcc ttagtggtgg ggtacgaaat aatgtctggc gtgcattttc taaccgcaat
     1201 tattcaatgg ctgtatgcaa tctcactggc agtaagaaag tccgcgagat gcggcatgca
     1261 acattgaaca gtgcgacgcg ttttatccac tttgtggaga accatccttt ccttatatca
     1321 ttgaaccgaa tgtctcctgc gaatgtc
//
LOCUS       DACADA010000362         1328 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.370, whole genome shotgun sequence.
ACCESSION   DACADA010000362 DACADA010000000
VERSION     DACADA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.370"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            15..629
                     /gene="evgA"
                     /locus_tag="ISB17_004907"
     CDS             15..629
                     /gene="evgA"
                     /locus_tag="ISB17_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAP1982726.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            634..>1328
                     /locus_tag="ISB17_004908"
     CDS             634..>1328
                     /locus_tag="ISB17_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP1982727.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVV"
BASE COUNT          420 a          276 c          252 g          380 t
ORIGIN      
        1 aaagggaata atctatgaac gcaataatta ttgatgacca tcctcttgct atcgcagcaa
       61 ttcgtaattt attgatcaaa aacgatattg aaatcttagc agagttgact gaaggcggaa
      121 gcgccgttca gcgggtggaa acacttaagc ctgatatcgt catcattgat gtcgatatcc
      181 ccggagttaa cggtatccag gtgttagaaa cgctgagaaa gcgccaatat agcggaatta
      241 ttattattgt ctccgctaaa aatgaccatt tttacgggaa acattgtgct gatgctggcg
      301 ctaatggttt cgtgagtaaa aaagaaggca tgaacaatat cattgcagct attgaagctg
      361 caaaaaatgg ctactgctat ttccccttct ctctcaaccg gtttgttgga agtttaacgt
      421 ccgaccagca aaaactcgac tccttatcga aacaagaaat tagtgtcatg cggtatattc
      481 ttgatggcaa ggataataat gacattgctg aaaaaatgtt catcagcaac aaaactgtca
      541 gcacttataa aagtcgcttg atggaaaaat tagaatgcaa atcactgatg gatctttaca
      601 cattcgcaca acgtaacaaa atcggctaac cacatgaagt ttttacccta tatttttctt
      661 ctctgttgtg gtctttggtc gaccataagt ttcgcagacg gagattacat cgaatatcgt
      721 ggcatcagta gtaacaaccg tgtcacactt gatccactac gtctgagcaa caaggaatta
      781 cgttggctag cgagcaaaaa aaatcttgtg attgcagtac acaagtccca aacagctacg
      841 ttgttgcata ccgattcgca gcaacggatt cgtggtatta atgctgatta tttaaatctt
      901 ttaaaaagag cgttaaatat caaattaaca ctccgggaat acgcagatca tcaaaaagca
      961 atggacgcgc tggaagacgg tgaagttgat atagtgttat cacatttagt tgcttcgccg
     1021 cctcttaatg atgacattgc tgcaactaat cctctgataa ttacctttcc ggcgctggtc
     1081 actacccttc acgattcaat gcgaccgctt acctcatcaa aaccagtaaa tattgctcga
     1141 gtagcaaatt atcccccaga cgaggtaatt catcaatcat ttccaaaagc aacaattatc
     1201 tcttttacaa atttatatca ggcattagca tccgtctcag ccggacagaa tgattacttt
     1261 attggtagta acatcattac cagcagtatg atttcccgtt attttactca ctccttaaat
     1321 gtagtgaa
//
LOCUS       DACADA010000363         1301 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.371, whole genome shotgun sequence.
ACCESSION   DACADA010000363 DACADA010000000
VERSION     DACADA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.371"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..897
                     /locus_tag="ISB17_004909"
     CDS             <1..897
                     /locus_tag="ISB17_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP1982728.1"
                     /translation="LKLSHNQDKSHAVHKALSYLADKTKEYSNGELVIRIYPNATLGN
                     ERESLELMNSGALQMVKVNAASLESFAPDYSLFSLPFLFRDRDHYYRVLQSDLGKKIL
                     KSSESKGFVGITYYDGGARSFYSNKPITKPEDLAGMKIRVQQSPSAIAMMKALGGVAT
                     PMAQGELYTALQQGVVDGGENNTVVYSDMRHAEVAKVYSRDEHTMVPDVLIISTDVLN
                     KLGDKERTALLKAADESMMQMKDVIWPAAEKEAYDKMKGMNATVVDVDKSAFKERVKP
                     LYDEFKAKDAQSAKNLELVESM"
     gene            939..>1301
                     /locus_tag="ISB17_004910"
     CDS             939..>1301
                     /locus_tag="ISB17_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1982729.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQIS"
BASE COUNT          388 a          259 c          287 g          367 t
ORIGIN      
        1 ttaaaactga gtcataatca ggataaaagc catgctgtcc ataaagcatt aagctattta
       61 gcggataaaa ccaaagagta ttctaatggt gaattagtta ttcgcattta cccaaatgcg
      121 acattaggca atgagcgtga atcactggaa ttaatgaact ccggtgcatt acagatggtt
      181 aaggttaatg ctgcatcact agaatcattt gccccggatt acagcctttt cagtctgccg
      241 ttcttattcc gcgaccgtga tcactattat cgtgttctgc aaagtgattt aggtaaaaaa
      301 atacttaagt catcagaaag caaaggtttt gttggtatta cgtactatga cggcggtgcg
      361 agaagttttt attccaataa accgattaca aaacccgaag atttagcggg aatgaaaatc
      421 agggttcaac aaagccccag cgccattgca atgatgaaag cactcggtgg tgtcgctacc
      481 ccgatggcgc aaggcgaact ctacaccgca cttcagcaag gagtggttga tggcggagag
      541 aacaataccg ttgtctattc cgatatgcgt cacgccgaag tcgcaaaagt ctattcacgt
      601 gatgaacaca ccatggtacc tgatgtgtta attatcagca ccgacgtgtt gaataaactt
      661 ggtgataaag agcgcacggc attattaaaa gcagccgatg agtcaatgat gcagatgaag
      721 gacgtcatct ggcctgcggc tgaaaaagaa gcctacgaca aaatgaaagg gatgaacgca
      781 acagttgttg atgttgataa atccgctttc aaagaacgcg tcaaaccgtt atacgatgaa
      841 ttcaaggcga aagatgcaca atcagccaaa aaccttgagc tagtagaaag tatgtaaccc
      901 taatgggcag gtaattcctg ccttttcatg gaagacttat gcgaacactc actcatattc
      961 ttaataagat ccttgccgga tgttgctgca ttattctcgc cattatggtg ttctgcgtaa
     1021 cctggcaagt tattgcacgt tttattttta acgctcccag cactgtgctt gatgagttca
     1081 cccagatttt atttatgtgg atgattttac ttggcggtgt atatactgcg gggttaaaaa
     1141 aacatctggc cattgatttg ttggctcaaa aacttccggc tgcatctgtt ctgacgcttg
     1201 acagttttat ccaaattatt attacggtct ttgcggttat tttcatgatc tatggcggga
     1261 atattgttgt tgaaaaagca caacatgttg gacaaatctc t
//
LOCUS       DACADA010000364         1255 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.372, whole genome shotgun sequence.
ACCESSION   DACADA010000364 DACADA010000000
VERSION     DACADA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.372"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            148..906
                     /locus_tag="ISB17_004911"
     CDS             148..906
                     /locus_tag="ISB17_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAP1982730.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(959..1066)
                     /locus_tag="ISB17_004912"
     CDS             complement(959..1066)
                     /locus_tag="ISB17_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982731.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          319 a          279 c          295 g          362 t
ORIGIN      
        1 ttattcaaaa aatgaaataa gaatacattg ctaagttatt cgcgcctttt tataattcgt
       61 tacctttcca gggacgctaa tctttatcct taagaaagtt tccgggaccg cgatggtctc
      121 tttttttgtt gtacggactc ttgataaatg aagcttttga agacagttcc tgccatcgtt
      181 atgttggcgg ggggcatgtt tgcgtcactg aatgcagctg ccgatgattc cgtttttact
      241 gtcatggacg atcctgccag cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat
      301 cttgcacaat caggcaatac gaaaagctcc tcacttacgg ctgacaccac tatgacctgg
      361 tatggacaaa ccactgcctg gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat
      421 gagcgttctt cagaaaaata tgcggcgggc gggcgtagcc gttttaattt aactgattat
      481 gactatttat ttggtcaggc aagctggctg acagaccgtt ataacggcta tcgagagcgc
      541 gatgtgttga ccgcgggtta tggtcgccag tttttgaatg gcccggtaca cagtttccgc
      601 tttgaattcg gccctggtgt gcgttacgac aaatataccg acaatgccag tgaaacccag
      661 ccgctgggct atgcctccgg ggcttatgca tggcagttga ctgacaacgc taaatttacg
      721 cagggtgttt cggtctttgg cgcggaagac acaacgctga actccgaaag cgcattaaat
      781 gttgctatta atgaacattt tggactgaag gtggcttaca acgttacatg gaactctgaa
      841 cctccagagt ctgcaccgga acataccgat cgtcggacaa ccttatcgtt gggttacagc
      901 atgtaaaatt atctgccgga ccgtgttatg cggtctggct gttttcgttt gtcactgctc
      961 agccccaaat cattgctgcc caacgtagca acaacatagc gccacagatt gccagtagca
     1021 ccaccaccat cttccagtgt tttttatcaa agcgtttagt cggcatacca gtgtcctgca
     1081 tatctcttgt gcgtgataac ggagtgtaac aaaagggaca ggcatcaagg gattttttgt
     1141 tattacagtc gtcgggtggc gttgtacacc tggtcactgt ttctgcattt tatgaaaaaa
     1201 attatgtatc gatatacaaa tatcataaat agcgggttat aaatacgtct ttagt
//
LOCUS       DACADA010000365         1218 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.373, whole genome shotgun sequence.
ACCESSION   DACADA010000365 DACADA010000000
VERSION     DACADA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.373"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(14..1165)
                     /locus_tag="ISB17_004913"
     CDS             complement(14..1165)
                     /locus_tag="ISB17_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HAP1982732.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          269 a          287 c          270 g          392 t
ORIGIN      
        1 cgcggttgta gattcaatct gtcaatgcaa cacccctttc aattatctct ttcggtgttt
       61 tgaacttcag tgtctttctc ggtctgttgt ttagctgagc agcaaccaga tctagttcat
      121 gttgagtata ttgggcaaga catgtctttt taggaaagta ctgccgaatt agcccatttg
      181 tgttctcatt tgttccccgc tgccaaggac tctgaggatc gcagaagtaa actttaacgc
      241 cggtgctgac agtaaattct agatgtctgg ccagttccat tcctctgtcc catgtcagtg
      301 attttctgag ttctgacggt aaactcagga atttgtcggt aagagcctga tttactgaga
      361 cagaatcttt gcccctgagt ctaaggatga tcgtataacg tgattttcgg tctacaagtg
      421 tggctatatg agagtttttt gtacctgaga ctaaatcgcc ctcccaatgt cccagagagc
      481 gtctgttatc gatatttcgg gaacgttcgt gaattggtgt tccgttcact atgttaatcg
      541 tacctctttc gcctttgcgg gtatgacgcc tgccatggcg aaggctatgc gaccgtcgca
      601 gatgctgtat attcaggtgg tgtagcgctt cacggctacg aaagtacagc gttttataaa
      661 ttgtctcagg tgatattcgc agcgtttttt gacgtggttt tgttcgcctt aaccatcctg
      721 atatttgctc tggagaccat ttcatctcca gcttttccag aacaagcttt cgcaatggta
      781 aattttgatc cagtaagcac ggttttggcc ttttcgccat tctgttggct cggttattag
      841 catcaacagc tttgtaatag cgtctgcccc gattacgctg aacttcacgt gagatcgtcg
      901 aaggactgcg attcagcgca gtagctatcg cacgaatgct cattttggct gacaaaccag
      961 ctcgtatctc ctcgcgctca gacagtgtca ggtgagctac agcccgctta cgctcatggg
     1021 gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt gaacccagga
     1081 tattcgctat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca gaggcttttt
     1141 cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt tttcacataa
     1201 ctgttgcgtt gaccaatt
//
LOCUS       DACADA010000366         1155 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.374, whole genome shotgun sequence.
ACCESSION   DACADA010000366 DACADA010000000
VERSION     DACADA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.374"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..543
                     /locus_tag="ISB17_004914"
     CDS             <1..543
                     /locus_tag="ISB17_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982733.1"
                     /translation="AELAFMSYFIFILTILPGLIYLKMNPQHKLSKRISSAFVASLMI
                     LLVISTQITVLPVIFTHSVIKLSGISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPG
                     EYYSVQAVSMFTTNQFILLCPKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVP
                     ISAISVKRWDMPLQGSKPSN"
     gene            complement(769..944)
                     /locus_tag="ISB17_004915"
                     /pseudo
     CDS             complement(769..944)
                     /locus_tag="ISB17_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          360 a          232 c          221 g          342 t
ORIGIN      
        1 gctgaactgg ctttcatgtc atattttatt tttattctga caatacttcc cggcctcatt
       61 tatctaaaaa tgaatccaca gcataaactt tctaaaagaa ttagttctgc atttgtcgca
      121 tccttgatga tattattagt tatatcgact caaataaccg tattaccagt aatttttacc
      181 cattcagtta ttaagctctc aggtataagt gacttcaaaa tacatagcta cattattaaa
      241 acaagtgaat atccggagga gtttttttcc aatgcagtat ggggtaaaaa aaatataaaa
      301 ccgggggaat attactctgt gcaggctgtt tctatgttta cgacaaacca gtttattctc
      361 ctttgtccca aggatattat aaggttttat cgggaaagct ggaagttcga cctgcttaat
      421 gtagacttcg atacaaatac caggaagaaa ctccaggaag aagcagcata ttgcgtacca
      481 atttctgcaa tatctgtaaa acgatgggat atgcctcttc agggctcgaa accctcaaat
      541 tagaaggccg ctcgtgcgac tgtattagat agtaatgcgg tatggaaaaa tagtgatggc
      601 aagatgaact tgacttggct aatcaaatac cggcgtagtg attaggaaac agggctcgga
      661 taacattatt gcgcggttca ttgaacctac tgatgacgcc ggatatatag tcgtaaaccg
      721 tttcgaacca gtacttatat caccaggaga gtccatatat gaccaccgtt tacagtaaaa
      781 acggtatctg ttttctgatc cctccagcgt caaactaccg ttgtaatggt gaggtctggt
      841 gctattgtaa taattcagaa tataatctct gatttatttc cgaacctcgc ccttgcctgc
      901 gtaaccatcc gtcgccccca tcctgttttc agactgcgga aaaaggcaag tttttatccc
      961 cccacagaga ttcaatcata tatcgcgtgt tttggaagtg acagttctta ttcctgagaa
     1021 tactgcatgt gcaacacact cggcatccat cgataccact tttatctcca ataggcagga
     1081 taaaaaacaa gactgtgaat taaagtaatc ggcaaatgaa tgagttagaa gatactgaaa
     1141 atgaatcaag gacta
//
LOCUS       DACADA010000367         1106 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.376, whole genome shotgun sequence.
ACCESSION   DACADA010000367 DACADA010000000
VERSION     DACADA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.376"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>1106
                     /locus_tag="ISB17_004916"
     CDS             <1..>1106
                     /locus_tag="ISB17_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HAP1982734.1"
                     /translation="KADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIVDLKDAKL
                     VIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEVQFLG
                     IFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNSIKES
                     WPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAAQQAELLW
                     RHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIALRVM
                     YEVAKEAGVVFDAISVADKELKVPSHLKVICEKAISQGKAVLLCTAPMPFSDDELKTI
                     GKYLHCSSNWNTVDYRLKNKVSAEVSAFKTFSFV"
BASE COUNT          313 a          211 c          274 g          308 t
ORIGIN      
        1 taaagcagac tctctggtag gaatggggct aggaaataat gatactgggg taattgccaa
       61 aactgaccgt gcgatatcac tgcttcgtga gacactggca aagattattg ttgatttaaa
      121 ggatgctaaa ctagtaatta agcgtctaca gtttgacgta tttggcttta gtcgtggggc
      181 ggcagccgca cggcacttta ctaatcgcgt atttgaacgt gatcccgtac tgattaaaac
      241 cattgcgacg gcatttcagt cagttgaata tttaggtaaa ccttccgggg aagtgcaatt
      301 tttaggtatt tttgacacag ttgcagccat cggagggctg atggatggtt ttgaccctca
      361 tgatggtaat aactttccgg taaaagtgaa gttaccgcca ggtgtggcca aacatgtatt
      421 ccagctcaca gcaatgaacg aatgtcgcta taacttttgc ctcaacagca taaaggaaag
      481 ctggccagaa ttaagtttcc ctggatcaca tgctgatatt ggtggcggct atgacccact
      541 ggaagatgag tacctgtttt taagtcgccc ggcgattgaa acggtactgg gaaatgtgcc
      601 ggtggaagcg acaagcgctt accaaaaagc agcacagcag gcagagttat tgtggcgcca
      661 ttcaacgctg gctccgattc tgccttctgg tgtcctaaaa attgaatcca atattgatga
      721 aatagcgtcg cctgaccact taggtaatgt gagaaaacgg gtcgctgcag cggtgacatt
      781 tcgcagaacg atcaccaatg actggtcaaa gattgcgtta agagtaatgt atgaagtcgc
      841 taaagaggca ggggtagtat ttgatgccat ttctgttgct gataaggaat taaaagttcc
      901 cagtcattta aaggtaatat gtgaaaaagc aatttctcag ggtaaagccg ttctcttgtg
      961 tacagcgccg atgccttttt ctgatgatga gcttaaaacc attgggaaat atttacactg
     1021 ctcttccaac tggaataccg tcgattatcg attaaaaaat aaggtgtctg ctgaagtatc
     1081 agcgtttaag acgtttagtt ttgtta
//
LOCUS       DACADA010000368         1072 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.377, whole genome shotgun sequence.
ACCESSION   DACADA010000368 DACADA010000000
VERSION     DACADA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.377"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..267
                     /locus_tag="ISB17_004917"
     CDS             <1..267
                     /locus_tag="ISB17_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1982735.1"
                     /translation="NRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPV
                     RPAVWLERDDDTGFQQVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            434..817
                     /locus_tag="ISB17_004918"
     CDS             434..817
                     /locus_tag="ISB17_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982736.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            814..999
                     /locus_tag="ISB17_004919"
                     /pseudo
     CDS             814..999
                     /locus_tag="ISB17_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          241 a          285 c          319 g          227 t
ORIGIN      
        1 aaccgggata cctggtgtcg tgccggcgat taccgcgctc cgggatcact ggccgggatg
       61 attaaacagg cgtggtgcag tgccctggga gtggatgccg gacgttatga cacgctggca
      121 cattttccgg tcaggccggc ggtgtggctg gaacgcgatg atgacaccgg ctttcagcag
      181 gtgctggagc gtgctgacta tctggcgaag gagagtacca aagcttacgg caccggtgag
      241 cgcaactttg gctgcagtcg tggctgagcc cgacagacga cttatctgac cttagtgtga
      301 tgacgtgttc acagcgacca cactgatttt cccttctgag actgaactga aaaccgccat
      361 tcacacctga ccacgctgcc cgtaccggga cggctgttgc ctgtgtgtcg tctggcgcag
      421 aggaacatat actatgtacg caaaatcttt tctcgcactt gatggcaacg gacgtctgac
      481 cggtgcccgt accgcacaaa ctgctcctta tgcccactac acctgccacc tttgcggcag
      541 tgcactcaga taccacccgc aatacgacac cgaacggccc tggtttgaac acactgacga
      601 cgggctgacg gagcacgcgc agcaatgtcc ttatgtcagg ccggaacgca gagaaatacg
      661 gttgattaaa cgtctgcagc aattcgtccc ggatgcctta cccgtggtac gtaaagccag
      721 ctggtactgc aggcaatgtc accacgatta ttatggggag cagtactgca cacactgcca
      781 gaccgggcgt ttcagtgagg agggggtagc agaatgaacg gtataaaaac ggggctgggg
      841 atcacgccgg gtgagcacat tatcagtgcc gactctgcgc tcagcagaaa tatccggcat
      901 tgtttctgcc tttcctgtca tggccggttg attctgcaga cagatgctca gggggcgtgg
      961 tttgagcatg atttacatgc cctgagtgag caacagaagg gtaagcgtgc agcaagaacc
     1021 gtattgacgg ggatgtgtta ttcagtcggc agtgctacgc gccaggggag ca
//
LOCUS       DACADA010000369         1065 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.378, whole genome shotgun sequence.
ACCESSION   DACADA010000369 DACADA010000000
VERSION     DACADA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.378"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(86..973)
                     /gene="rhtA"
                     /locus_tag="ISB17_004920"
     CDS             complement(86..973)
                     /gene="rhtA"
                     /locus_tag="ISB17_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAP1982737.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          318 a          280 c          260 g          207 t
ORIGIN      
        1 gcataattcg atatataaag acggtgtaga ggaaaagtag cgagaaaatc tgcatggtta
       61 tgcataacca tgcagaaatg taaatttaat ttatgtctaa ttcttttatt ttgctctctt
      121 tgcgtactgt cagcgtagaa cccattgaag cggcgatgat agcgccgagc gccagcagct
      181 gtatgggggt cagtgtttct ccgaggaaaa tcatcccgga aacggcagcc agagccggtt
      241 ccatgctcat tagcgtacca aatgtgcgtg ttggcaaacg ggtgagggca atcatttcca
      301 gcgaataagg cagagcggtc gagagaatag cgacagccag ccccaatgga ataaccgacc
      361 agtgccagag tgcttcacca gcctgaagcg ctccaattgg gacgaaaatt aacgctgcaa
      421 tcaacgaacc aattgccacc gtcgcagggc catgttccgc tcctgcgcgt tgcccactta
      481 aaatgtaaat agcccaacaa gccccggccc ccagtgccag cgcacagccg gttaaatcga
      541 catgggaaac gtcttgcccc agcggtagca ggaaccacag accaagaacc gccagcacaa
      601 cccagacgaa atctaccggg cgacgagaag agaacagtgc caccgccagt ggtccggtga
      661 actccagcgc caccgcaata cccagcggta ccgtctgaat agaaagataa aaaagataat
      721 tcattccacc cagcgaaacg ccgtaaaaca acagcggtaa tcgttgctct ttggcaaagc
      781 gcagtcgcca tggcttaaag aacgcgatga ggatcagcgt gcctaatgcc agacgcagcg
      841 cagtgacacc cggtgcgccc accagaggaa aaagtgactt agctaacgag gctccaccct
      901 gaatagacgc catggcaacg agcaatatga ctattggtaa ccagaccggc attttacgta
      961 atgaaccagg catcctttct cccacaaata tctaaaccta agtaaagcgt ggagtgtact
     1021 ggatataccc aatgctggtt gagcatttgt tgaaaaaatt ttccc
//
LOCUS       DACADA010000370         1058 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.379, whole genome shotgun sequence.
ACCESSION   DACADA010000370 DACADA010000000
VERSION     DACADA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.379"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>1058
                     /locus_tag="ISB17_004921"
     rRNA            <1..>1058
                     /locus_tag="ISB17_004921"
                     /product="23S ribosomal RNA"
BASE COUNT          300 a          231 c          326 g          201 t
ORIGIN      
        1 ttcgacacac tatcattaac tgaatccata ggttaatgag gcgaaccggg ggaactgaaa
       61 catctaagta ccccgaggaa aagaaatcaa ccgagattcc cccagtagcg gcgagcgaac
      121 ggggaggagc ccagagcctg aatcagtgtg tgtgttagtg gaagcgtctg gaaaggcgcg
      181 cgatacaggg tgacagcccc gtacacaaaa atgcacatat tgtgagctcg atgagtaggg
      241 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
      301 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      361 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcctcttaa tggggtgact
      421 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      481 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      541 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      601 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      661 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      721 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      781 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      841 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      901 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      961 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcaccgaagc
     1021 tgcggcagcg acactatgtg ttgttgggta ggggagcg
//
LOCUS       DACADA010000371         1037 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.380, whole genome shotgun sequence.
ACCESSION   DACADA010000371 DACADA010000000
VERSION     DACADA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.380"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(2..787)
                     /locus_tag="ISB17_004922"
     CDS             complement(2..787)
                     /locus_tag="ISB17_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1982738.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT          303 a          243 c          210 g          281 t
ORIGIN      
        1 attagcgcac taaataaccg ccatcaacca ctaataaatg tccgttgaca taattcgatg
       61 ccggacttgc gaggaatacg gctgccccca ttaaatcctg agtatcaccc caacggtttg
      121 ccggaatatg atcaagaaca cgctggttag tttctggatt actacgggtc gccagcgtaa
      181 tatcggttgc ataataacca ggagcaatac cattcacctg aatattatat tggcctaatt
      241 catcgcaata ggctttggtg aatccggcaa gagcatgttt agtggcagaa taagcaggtg
      301 accattgacc acctaagtaa gagaacaatg aacagatatt aatgatttta ccgcttttct
      361 gcgggatcat aatttttgcc gcttcgtagc ttaactcgaa tgcggcggtc aggttcacgt
      421 caatcatcgg gtcccagtcg gcacgaccaa agtccagcac cttattcagc ttacaaatac
      481 cggcattatt gaccagaata tcgacggtac cgaagcgctc acagcaggca gcgataatct
      541 tctgcggcgc gccttctgcg gtgatatcca cctgcataaa atcaacttca acgccctgtt
      601 tttcaatcat ttcctttgtt tcgccgttat ctttgacgaa actaggaata aagacatttg
      661 cgccagcttt ggccaacgcc atggcaaatg cctggcctaa accgctattc ccgccggtaa
      721 caattgctgt tttacctttc agggagaaaa aatccattga gaacgcattg agagattcga
      781 ttgacatagt tagctccaat ttccgtaagg caaaaaaaaa gaaaggtaac ttcccccgcg
      841 agagggaagt tacctttctc attatctctg gtaactggag ataattttta gcataaggac
      901 ataactgcta atgtgatcgt aatcacagtg tgatgttcgt attgtcaaat aatttaaaaa
      961 agcagaagcg tgattactct cacacaacac ctctgctttc catgttattt cttattgcaa
     1021 ttactagaga cctgaga
//
LOCUS       DACADA010000372         1035 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.381, whole genome shotgun sequence.
ACCESSION   DACADA010000372 DACADA010000000
VERSION     DACADA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.381"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(136..951)
                     /locus_tag="ISB17_004923"
     CDS             complement(136..951)
                     /locus_tag="ISB17_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP1982739.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          306 a          239 c          211 g          279 t
ORIGIN      
        1 tttatgagta aaggattatg tccacgataa gcacctgggt cgattcctgg gaggcggcca
       61 tgagggtagg gaagcgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca
      121 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      181 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      241 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
      301 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
      361 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
      421 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
      481 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
      541 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
      601 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
      661 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
      721 ttccgtcagc cagtttagtc tgaccatttc atctgtaaca tcattggcaa cgctaccttt
      781 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
      841 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
      901 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
      961 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1021 tgcaatgtaa catca
//
LOCUS       DACADA010000373          993 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.382, whole genome shotgun sequence.
ACCESSION   DACADA010000373 DACADA010000000
VERSION     DACADA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.382"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>993
                     /gene="evgS"
                     /locus_tag="ISB17_004924"
     CDS             <1..>993
                     /gene="evgS"
                     /locus_tag="ISB17_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP1982740.1"
                     /translation="LTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKV
                     LENPYSPPYSMTDETGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWD
                     ILPAAIYSEDRENNVSFAEVFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQL
                     KEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVQ
                     NASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYI
                     VTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVV"
BASE COUNT          301 a          206 c          195 g          291 t
ORIGIN      
        1 tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc
       61 ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc
      121 cgatttaaag gtgctggaaa atccttactc gccaccctat tctatgacag atgaaactgg
      181 ctcagttcgg ggcgtgatgg gtgatattct taatattatt accttgcaaa caggtttaaa
      241 tttttctccg atcaccgttt cacacaatat tcatgctggg acacagctta accccggtgg
      301 atgggatata ttgcccgccg ctatttatag tgaagatcga gaaaataatg tttcatttgc
      361 tgaagtcttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca
      421 aacattaaaa aaaggaatga aagttgccat tccatattat tatgagcttc attcgcaatt
      481 aaaagagatg tatccggagg tagagtggat aaaagtcgat aacgccagcg ctgcatttca
      541 caaggtcaag gaaggtgaac ttgatgctct ggtcgcgaca cagttaaatt cgcgttacat
      601 gatcgaccat tactatccta atgaacttta tcattttctt attcccggcg ttcagaatgc
      661 atcactttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc
      721 actgaatgca attcccccaa gcgaagttct gcgcctgacc gaaaaatgga ttaaaatgcc
      781 caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg ttacgacatt
      841 atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg
      901 tcgtcgtaaa gttattcagg gtgatttaga aaaccaaata tcattccgga aagcgctctc
      961 ggattcctta cctaatccaa cttatgttgt aaa
//
LOCUS       DACADA010000374          984 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.383, whole genome shotgun sequence.
ACCESSION   DACADA010000374 DACADA010000000
VERSION     DACADA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.383"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            86..652
                     /gene="slp"
                     /locus_tag="ISB17_004925"
     CDS             86..652
                     /gene="slp"
                     /locus_tag="ISB17_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAP1982741.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            805..>984
                     /locus_tag="ISB17_004926"
     CDS             805..>984
                     /locus_tag="ISB17_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1982742.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQ"
BASE COUNT          297 a          200 c          201 g          286 t
ORIGIN      
        1 tatcaggcat atcgcctcag aatcagatga aaactataaa gaaatatcta ttatggtttt
       61 aatatttgtt gataaggata gtaacatgaa catgacaaaa ggtgcactca tcctcagcct
      121 ttcatttttg cttgccgcat gtagttcaat tccgcaaaat atcaaaggca ataaccaacc
      181 tgatattcaa aaaagttttg ttgctgttca taaccagccg gggttatatg ttggtcaaca
      241 agcgcgcttt ggtgggaagg ttatcaacgt tatcaatggc aaaacggata cgttgttaga
      301 aatcgctgta ttaccgttgg atagctatgc gaagcctgat attgaagcca actatcaggg
      361 ccgactgctc gccagacaaa gcggcttcct tgatccagtg aactatcgta atcactttgt
      421 taccatcctc ggtaccattc agggtgaaca acctggcttt atcaataaag tcccgtataa
      481 cttcctcgaa gtgaatatgc agggcatcca ggtgtggcat ttgagagaag tggttaatac
      541 cacctataac ctgtgggatt acggctatgg tgcattctgg ccggaaccgg gctggggggc
      601 tccttactac accaatgcgg tgagtcaggt aacacctgag ctggtcaaat aacaccaccg
      661 aaagatgcag atgctttctc agcatctgca tcatgcatta catcaaatta atacacagta
      721 agctaactat tattataagc cctgtcctgt taattacctt tggcaaactg attataaagt
      781 taatgtccgt accaggagtc ggttatgttt cttataatta ccagggatac gatgtttttc
      841 accgcgatga aaaacattct gagtaaaggt aatgtcgttc atatacagaa cgaagaagag
      901 atcgacgtaa tgttgcatca gaatgccttc gtcattattg atacattaat gaataatgta
      961 tttcattcta attttctcac tcaa
//
LOCUS       DACADA010000375          973 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.384, whole genome shotgun sequence.
ACCESSION   DACADA010000375 DACADA010000000
VERSION     DACADA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.384"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..871
                     /locus_tag="ISB17_004927"
     CDS             <1..871
                     /locus_tag="ISB17_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1982743.1"
                     /translation="LLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVILPGQNLVVD
                     LSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNVLD
                     IGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATLGSGNPRNFTWNIISN
                     NSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQVF
                     ANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLGLSATYGQTGNKVAAG
                     AVQSVIGVTFIYE"
BASE COUNT          278 a          207 c          220 g          268 t
ORIGIN      
        1 tctattaatg gcaggcaaag tttttgcctt tagttgtaat gtcgatggcg ggtcaagtat
       61 tggagcaggt acaacctctg tttatgttaa tctcgatcct gtaatactac cgggccagaa
      121 tctggttgta gacttgtctc agcatataag ttgctggaat gattacggcg gctggtacga
      181 cactgatcat ataaacctgg tacaaggatc agctttcgca ggctccctac agtcatataa
      241 aggatcactg tactggaata atgtgaccta ccccttcccg ttgacgacaa ataccaacgt
      301 attggatatt ggtgataaga caccgatgcc attaccgtta aaactctata ttaccccagt
      361 cggagcagcc ggtggagttg tcataaaatc aggggaagtt attgcccgta ttcatatgta
      421 taaaattgcc acgttaggaa gcggaaatcc acgtaatttt acgtggaata ttatttcaaa
      481 taacagtgtt gttatgccaa caggcggttg caccgttgat agtcgtaatg tcaccgtgaa
      541 tctcccggac tttcctggaa gcgcagaaat accccttggt gtatattgct ctagcgaaca
      601 aaagctctca ttttatcttt cgggtgccac cactgacagt gcccgacaag tgtttgctaa
      661 cacagcacca gatgcaacaa aagcatcggg cgtgggtgtc tctctgatgc gtaatggaaa
      721 aacactggca acaggagaaa atgtctccct ggggacagta aataaaagca aagtacctct
      781 gggtttaagc gcaacctatg gacaaacagg aaataaggtt gcagccggag ccgtccagtc
      841 agtcattggt gtcactttta tctatgaata attgtgattt tgacattagt ctatgcgctc
      901 cattcacgaa aattgcgcgg ataattattt tgtgaaggct attatcctac acttgtaaga
      961 aaatccgcgc aca
//
LOCUS       DACADA010000376          972 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.385, whole genome shotgun sequence.
ACCESSION   DACADA010000376 DACADA010000000
VERSION     DACADA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.385"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>972
                     /gene="waaB"
                     /locus_tag="ISB17_004928"
                     /gene_synonym="rfaB"
     CDS             <1..>972
                     /gene="waaB"
                     /locus_tag="ISB17_004928"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HAP1982744.1"
                     /translation="AVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDKMDKGWLEG
                     IKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKARKKSGIDV
                     PVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIFNPVEAKDT
                     VIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGSDFEKCQAY
                     GRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESMSWGIPCIS
                     ADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKI"
BASE COUNT          278 a          162 c          225 g          307 t
ORIGIN      
        1 aagctgtttc tggatttggc ggcatggaaa cggtcattcg tgatgttatt actaccttca
       61 ggcaacaaca tatctatagt gaaatgtttt tcttttgtcg taatgataaa atggataagg
      121 gatggctgga aggtattaag tactcgtgtt cattttccaa tattcgctta ggttttttgc
      181 gtcgggcgaa acatattcat gctttaagca aatggcttca agattatcag ccagatgttg
      241 ttatttgtat tgacgttatc tcgtgtttgt atgcggccaa agcacgtaaa aaatcaggaa
      301 ttgatgtgcc agtattttca tggccgcatt tttctttaga tcacaaaaaa catgctgaat
      361 atatcacctg tgcagactat catttagcca tcagctctgg cattaaacag caaatgatta
      421 atcggggtgt accagaatcg acgataaatg ttatttttaa tccagtcgaa gctaaagaca
      481 cagtcattcc tgggcctgag gaaggtgaaa cggcaacgtt catttatgtt gggcgtatga
      541 aattcgaggg ccagaagagg gttaaagacc tgcttgatgg tttatcacaa gcgcaaggtg
      601 actggaaact ccatgtactg ggtgatggtt ctgattttga aaaatgccag gcatatggta
      661 gagaattgaa gattgataat cgaattgtct ggcatggttg gcaaaaacat ccatgggagg
      721 tagtgcgtca ggatattaag aaagtcagtg cattgttatt aacctcatct tttgaaggtt
      781 ttccaatgac cttacttgaa tcgatgtcat gggggattcc gtgtatcagt gccgattgtg
      841 tctccggtcc tggtgacatt attcaacctg atgtcaatgg ccatttgtat caaccgggag
      901 atattgctgg ttttgttact cttctaaata aatatatagc aggtgaaatt catatcgaac
      961 atgaaaaaat tc
//
LOCUS       DACADA010000377          947 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.386, whole genome shotgun sequence.
ACCESSION   DACADA010000377 DACADA010000000
VERSION     DACADA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.386"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..306
                     /locus_tag="ISB17_004929"
     CDS             <1..306
                     /locus_tag="ISB17_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP1982745.1"
                     /translation="APQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKA
                     SIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     gene            534..851
                     /locus_tag="ISB17_004930"
     CDS             534..851
                     /locus_tag="ISB17_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HAP1982746.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(801..>947)
                     /locus_tag="ISB17_004931"
     CDS             complement(801..>947)
                     /locus_tag="ISB17_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP1982747.1"
                     /translation="FSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAV
                     ASFS"
BASE COUNT          284 a          221 c          226 g          216 t
ORIGIN      
        1 gcgccacagc gcgaggagct ggccgaggtg gatgtggact ggctgatcgc cgagcgcccc
       61 ggcaaggtaa gaaccttgaa acagcatcca cgcaagaaca aaacggccat caacatcgaa
      121 tacatgaaag ccagcatccg ggccaaggtg gagcacccat ttcgcatcat caagcgacag
      181 ttcggcttcg tgaaagccag atacaagggg ttgctgaaaa acgataacca actggcgatg
      241 ttattcacgc tggccaacct gtttcgggcg gaccaaatga tacgtcagtg ggagagatct
      301 cactaaaaac tggggataac gccttaaatg gcgaagaaac ggtctaaata ggctgattca
      361 aggcatttac gggagaaaaa atcggctcaa acatgaagaa atgaaatgac tgagtcagcc
      421 gagaagaatt tccccgctta ttcgcacctt ccctagaaca acaatgcctc gattggttta
      481 aagagctagg ttaccaatac caaaatggct atgacatcgc accagatggt gatatgcctg
      541 aacgtgaaaa ctatcagcaa gtcacgctac aggcgcgttt agtctctgcc ttggaaacct
      601 taaacccaaa tgtgcccagt gacaccttaa tcgaagtagc gaaaatcgtt gccacaccgc
      661 aaacgcctgt actgattaaa aacaacaaac tctttcacca atatttaatc gaaggtgtgc
      721 ctgttgaata ctcggtaatg gagcaagaac aatgggtgac caagcatact catgttcgat
      781 taattgattt tgtgttgtgg tcaactaaaa ctggccaccg cgttagagtt tttccagtat
      841 cggttttctg attcgtttgg tggtaaccca ccattatatt cgtgcggtct tagtgcgctg
      901 taatatccaa cgatatagtc cgttatggcg tgagctgcat cgctgaa
//
LOCUS       DACADA010000378          943 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.387, whole genome shotgun sequence.
ACCESSION   DACADA010000378 DACADA010000000
VERSION     DACADA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.387"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            123..809
                     /locus_tag="ISB17_004932"
     CDS             123..809
                     /locus_tag="ISB17_004932"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP1982748.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT          258 a          197 c          238 g          250 t
ORIGIN      
        1 ttttttaagc taagtaaagg gctttttctg cgacttacgt taagaatttg taaattctca
       61 ccgcgtaata agttgacggt gatcaccctg ttcgcggtta tttggtcaag aagagtggca
      121 atatgcgtat aacgattatt ctggttgcac ccgccagagc agaaaatatt ggggcagcgg
      181 cgcgggcaat gaaaacgatg ggttttagcg aactgcggat tgtcgatagt caggcgtacc
      241 tggagccagc cgcccgttgg gtcgcacatg gatctggtga tattattgat aatattaaag
      301 ttttcaagac actagctgaa tcgttacacg atgtcgattt cactgtcgcc accactgcgc
      361 gcagtcgggc gaaatatcat tactacgcta cgccagtaga actggtgccg ctgttagagg
      421 aaaaatcttc atggatgagc catgccgctc tggtgtttgg tcgcgaagat tccgggctga
      481 ctaacgaaga gttagcgttg gctgacgttc ttactggtgt gccgatggtg gcggattatc
      541 cttcgctcaa tctggggcag gcggtgatgg tctattgcta tcaattagca acattaatac
      601 aacaaccggc gaaaagtgac acaacggcag accaacatca actgcaagct ttacgcgaac
      661 gagccatgac attgctgacg actctggcag tggcagatga cataaaactg gtcgactggt
      721 tacaacaacg cctggggctt ttagagcaac gagacacggc aatgttgcac cgtttgctgc
      781 atgatattga aaaaaatatc accaaataaa aaacgcctta gtaagtgatt ttcagctttt
      841 cattctgact gcaacgggca atatgtctct gtgtggatta aaaaaagagt gtctgatagc
      901 agcttctgaa ctggttacct gccgtgatca aattaaaatt tta
//
LOCUS       DACADA010000379          936 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.388, whole genome shotgun sequence.
ACCESSION   DACADA010000379 DACADA010000000
VERSION     DACADA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.388"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            92..>936
                     /gene="tdcA"
                     /locus_tag="ISB17_004933"
     CDS             92..>936
                     /gene="tdcA"
                     /locus_tag="ISB17_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAP1982749.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYR"
BASE COUNT          253 a          183 c          216 g          284 t
ORIGIN      
        1 tgaaagttaa tttgtgagtg gtcgcacata tcctgttcat ttcattttga tacacttcat
       61 accgtcaatg aggtaattaa cgtaggtcgt tatgagcact attcttcttc cgaaaacgca
      121 gcacctggta gtctttcagg aagttattag aagtggttct atcggctcgg ctgcaaaaga
      181 gttagggtta actcaaccgg ccgtcagtaa aatcattaac gatattgaag attattttgg
      241 ggtggagtta gtggtgcgga aaaataccgg tgtaacatta acacctgccg gtcaattgtt
      301 actctcccgt tccgaatcca ttacccgtga aatgaaaaat atggttaatg agataagcgg
      361 tatgtcttct gaggcggtgg tggaagtctc atttggtttt ccttcattga ttggttttac
      421 ttttatgtca gggatgatca acaagttcaa agaggtgttc ccgaaagcgc aggtttctat
      481 gtatgaagcg caactgtctt cgttcttacc ggcaatccgc gacggtcgac tggattttgc
      541 gattggtacg ttaagcgcag aaatgaagct tcaggattta catgttgagc cgctgttcga
      601 gtccgagttt gtgctggtag ccagtaagtc ccgaacatgc accggcaccg ccacgctgga
      661 gtcgttgaag aacgaacagt gggtgttgcc acaaaccaat atggggtact acagcgaact
      721 acttactacg ttacaaagaa atggcatcag tattgaaaac atcgttaaaa ccgactcagt
      781 cgtgacaatt tataatcttg ttctcaatgc tgatttctta actgtaattc cttgtgatat
      841 gacatcacct tttggttcta atcaatttat tactattccg gttgaagaaa cattacctgt
      901 ggcacaatat gccgcggtat ggtcgaaaaa ttatcg
//
LOCUS       DACADA010000380          932 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.389, whole genome shotgun sequence.
ACCESSION   DACADA010000380 DACADA010000000
VERSION     DACADA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.389"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..459)
                     /locus_tag="ISB17_004934"
     CDS             complement(<1..459)
                     /locus_tag="ISB17_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1982750.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIAT"
     gene            complement(513..914)
                     /locus_tag="ISB17_004935"
     CDS             complement(513..914)
                     /locus_tag="ISB17_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982751.1"
                     /translation="MIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPEGVI
                     AQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIACFM
                     SDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
BASE COUNT          279 a          218 c          193 g          242 t
ORIGIN      
        1 cgtagcaata accgcaaata atgcaccgat gatgcgataa ggggtaaagt attttttctc
       61 gtcgacaccg ataccaaagc gatcgcaaag taaaccagac agcaatagcg cggagatcag
      121 tgcggtttga aacgttgcga ccccgagtgc gctggctgat gcgccttcag aaaataccac
      181 catcgctcca cagagaccgg caaaccagtt ccagagagga acttttcggt ttttaatcag
      241 agaaggaatt gccgcaaatt gttgccgggt ttcttttcgc gccataataa taaaaaacat
      301 cacgactaaa ccactggcaa aggagataac tgcgcatgca ttaccatcct gtaaccagtg
      361 acctaattga ccattcaccg ctgattgcat aggggagagc atacccgcaa gaatcgtggc
      421 aagcatccat aagggagttg agtacttatt ctttttcata catcaatcct tctattatag
      481 taaatggggt aactggagtt accccattat tattatcttg caatatgagt cagtatattt
      541 acccacaacg cggtatagaa cggccaggac ataaactgct gtgtacccca gtgcggtgaa
      601 cagtcactca taaagcaggc tattttcccg ttgtgatagt taccaaagac cagtaatgga
      661 tcgttgttta ttgttaatac cacctcagca ttttctttag cgatcgcccg gttataacct
      721 aggaaaaaag gatattcaga gaacccttta ataactggat gttcaggttg tgatggctgt
      781 gcaataacac cctctggttt ttcgacacga tcgtcaccat ctaacatcgt aaccgggagc
      841 acatcagcca gaactgtatt tttataattg gcttttgctt caattcccat gaatgagaga
      901 tagccgccaa tcatcaataa cccaccaccg tt
//
LOCUS       DACADA010000381          899 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.390, whole genome shotgun sequence.
ACCESSION   DACADA010000381 DACADA010000000
VERSION     DACADA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.390"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(111..743)
                     /locus_tag="ISB17_004936"
     CDS             complement(111..743)
                     /locus_tag="ISB17_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAP1982752.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          214 a          213 c          185 g          287 t
ORIGIN      
        1 aaccgcgagc gccaaataat tttatgacca caacctgtcg tattgtggtc ataaaaaatt
       61 gtcggctgga gggagttaaa tcctttacct caatatattg cctgtcaggt ttagttcaca
      121 aacagtgctt cgctatcaac gatgattcca tcttccgcac ctttaagcgc caggtttgcc
      181 tcgtagtatt gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt
      241 ttgagcggca tcagatattg gttttccatt acgccaacac cggccaggcg cagctcttcc
      301 attgcgccct ttttatcccc tttcgccatt ttttcgttgg caatttttat cgcggcttct
      361 ttttcgggtg tcgctacata actctcagaa attccgacag atgcattaat gacaatatat
      421 tggtcatcat tgacgttggt ctttttacct ggcttagcga actttgccca ttcggtacta
      481 tcatctgaca gcaaagcgga agcttcattg gtcagttctt ttgccttttc tggatcgcca
      541 tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag
      601 atacgctctg ctgctgccgt tttctgggtt tgcttaactt gctgagtttg ctgttgtgcc
      661 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
      721 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
      781 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
      841 atcttacaac tgtagttatt atcgcaaagc cgcttttagt cttacagttg taggaatct
//
LOCUS       DACADA010000382          892 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.391, whole genome shotgun sequence.
ACCESSION   DACADA010000382 DACADA010000000
VERSION     DACADA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.391"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(1..345)
                     /locus_tag="ISB17_004937"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="ISB17_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(394..741)
                     /gene="tnpB"
                     /locus_tag="ISB17_004938"
     CDS             complement(394..741)
                     /gene="tnpB"
                     /locus_tag="ISB17_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1982753.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          181 a          240 c          255 g          216 t
ORIGIN      
        1 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
       61 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tatcgctctc
      121 tttctgaagc cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga
      181 acgactgccg aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc
      241 gcgttcacgc tcgttcagct tttccagcag ggcacggttc agcgcctcct gttcggcaag
      301 gagacgtttc agtgcattga tatcgtcagg aagtgagctg ctcataccgg gtatattacc
      361 aggctcattc agcgtcgacc aggataaaga ggcttacaac atagtcaggg acgtaagcag
      421 tcttttaggc tgccgccagt cgataccttc aaggagcatc gccagctgtg ccggtgtgag
      481 gaacactttg ccatcccggg ctgacggcca ggcgaagcgg ccgcgctcca gccgtttggt
      541 cagcagacac agtccatcgc cggtagacca gaggagcttt acctgactgc cattacgccc
      601 acggaagata aaaacgtgac ctgacatcgg atcgtctttc agcgtcgtct gcacctttgc
      661 cgccaggccg ttgaagccgt ttctcatatc ggtgatgcca gcgaccagcc agatctttgt
      721 ccctgatggt aatgggatca acgtttgagc tcccgtatca gcaggttcag gatattttcg
      781 ctgatggcac cattcagacg gagtgatccg tgccggaacg ttacttcaca gctgatattg
      841 agtgattcag gtgtcgcagc agctacaggt tgtgaccggg gggaggttgt ag
//
LOCUS       DACADA010000383          890 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.392, whole genome shotgun sequence.
ACCESSION   DACADA010000383 DACADA010000000
VERSION     DACADA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.392"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..313)
                     /locus_tag="ISB17_004939"
     CDS             complement(<1..313)
                     /locus_tag="ISB17_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982754.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WS"
     gene            complement(433..825)
                     /locus_tag="ISB17_004940"
     CDS             complement(433..825)
                     /locus_tag="ISB17_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 domain-containing protein"
                     /protein_id="HAP1982755.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
BASE COUNT          235 a          220 c          194 g          241 t
ORIGIN      
        1 gggaccagtg aaactggtcg ctgagcataa tcaggcgaga gtggtcagtg acgtcaagaa
       61 accgctgcat ttccctgaac agccgcccgt ctgttaaccc ccgcttccct gcaaagggtc
      121 tggtgatttc aaccgggaac tggcggcggt aatgcccttt ctgcaacacc tgagctatta
      181 accagtctaa gcggtcttct tcaattcctt cagtcagcca ttcatgcaaa ggccagcgct
      241 gctgataagt ctgaataagc cagttcatta acgggcgtcg tttcaccctg ccgtcataca
      301 gccagctcag catgttaacc acgcaataag caccgcaaag acagtccagt tcgccctgtt
      361 gagacgcacg tatccctctg tcagtcgtca cagataattc cccataaatt agtgatggtt
      421 agtacagggg gattagcttt cccagagaac ggtaatggtg tagatgttca tgccgtgata
      481 ctgaataaag ttggattgcc agagttcaaa cagctccctt ccggcgttaa caggcaggtc
      541 accaacataa gcaagaaaga catattgctg actccagctg atatcaattt ccttttcaag
      601 ttcggggtat accgtcccca tataactgaa gtcggtaatg taatcaaaat accattcatc
      661 attgcgtgaa ataatccgta tttcgacagg atggtaacca cccttttcag cgctgtaact
      721 caggtcttta aataacagac tgacggctgt aattttggct ttatgcggcg gtggatttcc
      781 cgcttcattt aatatgatat ttattaattc ctgatcgacg ggcatagcta gcccttcctg
      841 atggacaata ttcatcagtg attttcctgt tcaatatatt ctgataagac
//
LOCUS       DACADA010000384          888 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.393, whole genome shotgun sequence.
ACCESSION   DACADA010000384 DACADA010000000
VERSION     DACADA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.393"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            19..>888
                     /locus_tag="ISB17_004941"
     CDS             19..>888
                     /locus_tag="ISB17_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP1982756.1"
                     /translation="MLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTIEQFYVLPYM
                     LSTDHIIRQAVITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASSNWQDPGS
                     YVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTPGFFLSTSIKDKGKIVGVVVVKISLN
                     EIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMRTLQPLPVQAKQKLQSTRQYSLDNLL
                     PADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQIAIPEFNWKMTIMVPLDNLYWSWAI
                     SLVITLIIYLLFLL"
BASE COUNT          274 a          193 c          153 g          268 t
ORIGIN      
        1 atttcacttt ttcttatcat gctcgcggtt tcaatcgctt ttttgcactt gcgttttaat
       61 accttgtccg gtaccgataa aatgcggctt gaaatgtata agtccacatt atattccacc
      121 atcgagcaat tttatgtttt accctatatg ctctcaacag accatatcat ccgtcaggcg
      181 gtaattacgc ctgacgatat gacgtccagc gaactcaatc aacgaattgc acatttcaat
      241 actcaactca aaaccgcagc aatatttatt ctggataccc aaggtaaggc catcgcttct
      301 agtaactggc aggaccccgg cagctatgta gggcaaaatt atagctatcg cccctattat
      361 aaacacgcca tgtctggctt aaatggacgc ttttacggta ttggtagcac tacgaataca
      421 ccgggattct tcctctctac aagtataaaa gataaaggaa aaattgtcgg tgttgtagta
      481 gtaaaaataa gtcttaatga aattgaaaaa gcatgggccg aaggtcctga aaatattatc
      541 gtgaatgatg aacatgggat tatattttta agttcaaaat cgccatggcg aatgcgaaca
      601 ctgcaaccgt tacctgttca ggcaaaacaa aaactacaat ctacccgcca atatagtctc
      661 gacaatcttt taccggcgga ttattatccc tgttataccg tgagcaattt tactttcctg
      721 aaagataaaa aagaacaact ctgtttattc ccgcaatatt atacgcaaca aatagccatt
      781 ccagaattta actggaaaat gacaattatg gtccccttag ataacctgta ctggtcatgg
      841 gctatttcgt tagtcattac actaattatt tacctgctgt ttttgtta
//
LOCUS       DACADA010000385          881 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.394, whole genome shotgun sequence.
ACCESSION   DACADA010000385 DACADA010000000
VERSION     DACADA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.394"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..719
                     /gene="ydeO"
                     /locus_tag="ISB17_004942"
     CDS             <1..719
                     /gene="ydeO"
                     /locus_tag="ISB17_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAP1982757.1"
                     /translation="ANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIK
                     KYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSI
                     FAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQ
                     TTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKE
                     YRCQRHTGMNTDNTMNALAS"
BASE COUNT          271 a          177 c          175 g          258 t
ORIGIN      
        1 acgctaacat tttagataaa gattacgcct tctctgacgg cgagatcctg atggtagata
       61 acgctgttcg cacgcatttt gaaccttatg agcggcattt caaagaaatc ggatttaatg
      121 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg atgcctgttc
      181 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
      241 atctcaactc ggaagaacgc aatcatcata atttttcaga gcttttgctt ttttcttgcc
      301 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
      361 tttccgggaa agtaagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
      421 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
      481 aaacgacatt ctcacagatt cttttagatg ccagaatgca gcacgcgaaa aatttgatac
      541 gcgtagaagg ttcagtcaat aaaattgctg aacaatgcgg ttatgccagt acatcttatt
      601 ttatttatgc gtttcgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
      661 gttgtcaaag gcacacgggt atgaacacgg acaacacgat gaatgcttta gctagttgat
      721 tatttgctaa cgagtagtca accacacacg ctgcgtaaga attaaatggg gcagccattc
      781 cctgccccgc gttgtttcca ggcgatatat ttcttgaaat aaataagtga cagccatcac
      841 atatttatgc attttcataa cctgttgcat gattatttat g
//
LOCUS       DACADA010000386          875 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.395, whole genome shotgun sequence.
ACCESSION   DACADA010000386 DACADA010000000
VERSION     DACADA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.395"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            10..426
                     /locus_tag="ISB17_004943"
     CDS             10..426
                     /locus_tag="ISB17_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP1982758.1"
                     /translation="MQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLS
                     PSEISQKLQFTRTNITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLA
                     QSMYLKEIWDYLTHDEQELFEVINKKLLAHFSDASS"
BASE COUNT          291 a          155 c          187 g          242 t
ORIGIN      
        1 catttgttga tgcagctctg tattcgcaca aataaaagaa tgcaggataa tatatctgaa
       61 tttctggggg tgtatggaat aaatcactca gcatatatgg ttctcaccac attattcgca
      121 gcggagaacc attgtctgtc accttcagag ataagccaga aacttcagtt taccagaact
      181 aatattaccc gcattacaga ttttttagaa aaagccggat atgtaaaaag gacggatagc
      241 agggaggatc gccgtgctaa aaaaatcagt ctgacatctg aaggtatgtt ttttattcag
      301 aggctcactc ttgcacaaag catgtatctg aaagaaatct gggattatct gacccatgat
      361 gaacaggaac tgtttgaagt cattaataaa aagttactgg cacatttttc tgatgccagc
      421 tcataaagtg cgaaatatct gaggatgctg gatagcttca ggcaagagaa taatgattct
      481 tgcagatgtg tttttccgga tacaaaaaca aaagtaaaaa attgcagcgc caggccacct
      541 ttcaaagcag ggaggcctgt accgcgtcga aaatttcagc cagttaatgt aattgtctga
      601 accaggcact tcgcccgggc aggagaagga gcgtggttgc ggtcatcctg tatactgaag
      661 tcgttatgaa ggaataatac aaataattgc ccgttttctc tgtacgatag aggcaggtgt
      721 gtatgattgc attactatgt ttttgtgata taaaaaacta aatggtgaga tatgcatttt
      781 aaaatataac aaaaatcacc atgaaataag aataattatc cacggagaac ataaactgca
      841 gactgttaca atgcatgctg ttcatcttga caatg
//
LOCUS       DACADA010000387          868 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.396, whole genome shotgun sequence.
ACCESSION   DACADA010000387 DACADA010000000
VERSION     DACADA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.396"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..773)
                     /locus_tag="ISB17_004944"
     CDS             complement(<1..773)
                     /locus_tag="ISB17_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HAP1982759.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     DSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDG"
BASE COUNT          218 a          191 c          196 g          263 t
ORIGIN      
        1 ataccgtcct gaataacttt cccgttatta ccggcgatct ccgtgccagt ggaatcttta
       61 ccatcaacga tggttttgcc attgttgttt actgtggcat tatcacccgt aataacaagc
      121 tcaccgttat gattatattg ccacttcgtt gcgccatctg cgctcacccc gtcaagggca
      181 accgtgttat tcgcttcatc aatttgccaa atattaatag ttgccttacg gccattgcta
      241 tcttgtagag aaatagtccc gtcagcattc tcggtataag taaaaactga atcgcgaata
      301 gttaacgttt ttgctgtttt atcgaggata acgtcattat taaacgaaat tggtttggga
      361 ggcgtcgggg tatcatccga accgtcgtct ggtattatct catctccgcc aggagttggg
      421 ggaactggag tgtcgtctga attatcatca ggtggagttg gtggaagtgg agaattattg
      481 cgatgatgat gatcatcatc gtcgtttagt tccactatgg ctaaggctgt gattaacgca
      541 gtagcagcac ctgtggcaag ccatggcatc aggttctgtt cgactacagg ggagcattca
      601 gagggtaagc gtttaagctc ttctggactg agttttccag ggtctgccgg acaagtcaca
      661 ttggtatttt tcttttcacc tgtggtggtt tctatctctg tgatatctgc cgcccagccc
      721 tgaccactca gagccaatgc aatacaggct gacaatagag ttttcctttg cataataaat
      781 ccttctggcg aatataaaga aggtcggatg ccttaaccca accaattaat caatcaatta
      841 aaacatagag gacattcaat attgccag
//
LOCUS       DACADA010000388          862 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.397, whole genome shotgun sequence.
ACCESSION   DACADA010000388 DACADA010000000
VERSION     DACADA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.397"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>862
                     /gene="ompT"
                     /locus_tag="ISB17_004945"
     CDS             <1..>862
                     /gene="ompT"
                     /locus_tag="ISB17_004945"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HAP1982760.1"
                     /translation="RAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGKT
                     KERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVD
                     QDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSF
                     TARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFK
                     YSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVT
                     NKKGNTSLYDH"
BASE COUNT          268 a          181 c          202 g          211 t
ORIGIN      
        1 cgggcgaaac ttctgggaat agtcctgaca acccctattg cgatcagctc ttttgcttct
       61 accgagactt tatcgtttac tcctgacaac ataaatgcgg acattagtct tggaactctg
      121 agcggaaaaa caaaagagcg tgtttatctg gccgaagaag gaggccgaaa agtcagtcaa
      181 cttgactgga aattcaataa cgctgcaatt attaaagggg caattaattg ggatttgatg
      241 ccccagatat ctatcggggc tgctggctgg acaacactcg gcagccgagg tggcaatatg
      301 gtcgatcagg actggatgga ttccagtaac cccggaacct ggacggatga aagtagacac
      361 cctgatacac aactcaatta tgccaacgaa tttgatctga atatcaaagg ctggctcctc
      421 aacgaaccca attaccgcct gggactcatg gccggatatc aggaaagccg ttatagcttt
      481 acagccagag gtggttccta tatctacagt tctgaggagg gattcagaga tgatatcggc
      541 tccttcccga atggagaaag agcaatcggc tacaaacaac gttttaaaat gccctacatt
      601 ggcttgactg gaagttatcg ttatgaagat tttgagctag gtggcacatt taaatacagc
      661 ggctgggtgg aagcatctga taacgatgag cactatgacc caggaaaaag aatcacttat
      721 cgcagtaaag tcaaagacca aaattactat tctgttgcag tcaatgcagg ttattacgta
      781 acacctaacg caaaagttta tattgaaggc gcatggaatc gggttacgaa taaaaaaggt
      841 aatacttcac tttatgatca ca
//
LOCUS       DACADA010000389          822 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.398, whole genome shotgun sequence.
ACCESSION   DACADA010000389 DACADA010000000
VERSION     DACADA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.398"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..802
                     /locus_tag="ISB17_004946"
     CDS             <1..802
                     /locus_tag="ISB17_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjhT family mutarotase"
                     /protein_id="HAP1982761.1"
                     /translation="LQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGV
                     NENIFNGYFSDVELSKGNSALTEKVNRDYFSKPADDYFLNNHIISYDPSKNQWKNLGT
                     TPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGV
                     SGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVG
                     SLPEGLAYGVSLPWQGGMLILGGEKKDGKAVSDVIYLKKNDKQIKIVK"
BASE COUNT          249 a          135 c          202 g          236 t
ORIGIN      
        1 cttacaaaaa gatgtctatt cctatgatat tgctaaagat aaatgggaaa aattgatgac
       61 ccgtccccct gtatctcttg ccggtcatgt ttcttttatc cataatggtc atgcggtatc
      121 tactggtggt gtaaatgaga atatttttaa tggatacttt tcagatgtcg agttatcaaa
      181 agggaattcc gcgctgacag agaaagtcaa cagggattat tttagtaaac ctgctgatga
      241 ttatttttta aataatcata taatttctta tgacccgtca aaaaaccagt ggaagaacct
      301 gggaacgact ccatttccgg gaactgctgg ttcatcggtg atttttgcag aacagcaaat
      361 ttatatactg ggaggggaga gaaaaccggg gcttcgttct gttagatcct ggacggggga
      421 gctttcgcat gatcgaatta aatggtctga acttccacca gtagcttcac ccgagggggt
      481 gtcaggagca tatgcctctg ttattgatgg aaatatcttt ctggccggtg gtgcatattt
      541 cccgggggcg gcagaaaagt atagcaatgg tgaatactgg tctcataaag gactggataa
      601 agcctattca aaagagatat atcagctaat aaaaaatgac tggaaaaaag ttggttcttt
      661 gccggaagga cttgcttatg gtgtaagcct gccctggcag ggggggatgc tcattctggg
      721 gggggagaaa aaagatggta aagctgtttc agatgttatt tatctgaaaa aaaatgacaa
      781 acaaataaag attgttaaat aactgtaatg caggtgactg ta
//
LOCUS       DACADA010000390          790 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.399, whole genome shotgun sequence.
ACCESSION   DACADA010000390 DACADA010000000
VERSION     DACADA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.399"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>790
                     /locus_tag="ISB17_004947"
     CDS             <1..>790
                     /locus_tag="ISB17_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982762.1"
                     /translation="AVSELASRVSSKTGRKLLAIFIVSALSYGAAEYACAAQMDTRNF
                     WIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPDFSPVSAKGATTAIGNAY
                     SVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLDKFVVETAGATEHADFNL
                     SAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYNPLLRSASMFQLNWNNMY
                     ASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKVFSSK"
BASE COUNT          232 a          149 c          187 g          222 t
ORIGIN      
        1 gctgtatcag aattagcatc ccgagtatcc tctaaaaccg gtcgaaaact tctcgctata
       61 tttatagttt ctgccttgtc ttatggtgcg gctgaatatg cctgtgcagc acagatggac
      121 acccgaaatt tctggatccg tgattatctt gatctggcac agaataaagg tgcatttcag
      181 cccggagcat acggggtaaa aacgccatta aaaaatgggg gagaattcag ttttcctgaa
      241 gtaacaatcc ctgatttttc tcctgtatcc gctaaaggtg caacaactgc tattggtaat
      301 gcctacagtg ttacagcaag tcataatggc actattcacc atgccattaa aactcagaca
      361 tggggacagt cagattatca ttatgttgat cgtgtgacca aaggtgactt tgcggtccag
      421 cgtctggata agtttgttgt tgaaacagcg ggtgcaacag agcatgctga tttcaactta
      481 tcagcagcag aagcactaga gcgttatggt attgaattca atggaaagaa acaaataatc
      541 ggtttccgcg ttggagctgg tgcaaccggt gttacatctt atggggtggg acagacatac
      601 aatccattat tacgtagtgc ctccatgttt cagttaaact ggaacaatat gtatgcaagt
      661 aataataccg gtgggtttta taatgaagtg acaggaggtg acagcggttc cggattttac
      721 ttgtatgata atcagaaaaa aaaatgggtc attttgggga cactgacagg taaagtattc
      781 tccagtaagg
//
LOCUS       DACADA010000391          755 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.400, whole genome shotgun sequence.
ACCESSION   DACADA010000391 DACADA010000000
VERSION     DACADA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.400"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>755
                     /gene="vgrG"
                     /locus_tag="ISB17_004948"
     CDS             <1..>755
                     /gene="vgrG"
                     /locus_tag="ISB17_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1982763.1"
                     /translation="ARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNT
                     AYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAE
                     SGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFRKGVLITGVTAS
                     AARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKDGRY
                     RVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGL"
BASE COUNT          177 a          227 c          227 g          124 t
ORIGIN      
        1 acgcccggct gaaaatcgaa gttgtcgagt tttatgacga ccagagcggc tacgagcgcg
       61 ggctgacact gccgcttcgc catccttcag ggctgtttga tggtgaaacg gaagcggtat
      121 gggggctgaa taccgcgtac agcgtggtgg aaaaaagcgt caccactcgg gactacaact
      181 accgcaccgc cacagcagag atgatgacag agcagcatga cgccaccggg ggcgataaca
      241 ccacctacgg cgaagcctac cactatgccg acaacttcct gcaaaaaggc gataaagagg
      301 cagcggaaag cggcgcgttt tatgcccgta tccgccacga acgctacctg aatgaacagg
      361 ccattctcaa aggccagagc accagttcac tgctgatgcc ggggctggaa atcagggttc
      421 agggcgacga cgcgccagca gtgttccgca agggcgtact gattaccggc gtgacggcca
      481 gcgcggcgcg tgaccgcagc tatgagctga cgtttaccgc catcccgtac tcagagcgct
      541 acggctaccg cccggcgctt atcccccgcc cggtaatggc cggcacactc ccggcgcggg
      601 tcaccagcac ggtgaaaaac gatatctatg cccatatcga caaggacggg cgctaccggg
      661 tcaacctcga ctttgaccgc gatacgtgga aaccgggtta tgaaagcctg tgggtgcgcc
      721 agtcgcgccc gtatgcaggt gacacgtatg gcctg
//
LOCUS       DACADA010000392          755 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.401, whole genome shotgun sequence.
ACCESSION   DACADA010000392 DACADA010000000
VERSION     DACADA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.401"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..649
                     /gene="gadX"
                     /locus_tag="ISB17_004949"
     CDS             <1..649
                     /gene="gadX"
                     /locus_tag="ISB17_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAP1982764.1"
                     /translation="SLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          215 a          147 c          175 g          218 t
ORIGIN      
        1 ttcattattt ctcccgatgc tcaaggagga ggcattaaaa cttcatgtac ataaaaaagt
       61 tccttcatta ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca
      121 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt
      181 tgcgttgtta tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt
      241 gcaaccgaat atgcgaacac gagtttgtac ggtcatcaat aataatatcg cccatgagtg
      301 gacattagcc cgaattgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt
      361 gcgcgaagaa ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt
      421 acaacttatt gttatatatg gggtttcaat taagcgagtt gcagtatcct gtggatatca
      481 cagcgtgtcg tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta
      541 tcaggagcga tcggcgcagg aattaccgaa ctgtggctcg gcggcaagta tggctgcgca
      601 agggattttt tacggcactg accgttctgc ggaaggaata agattataga gttttactca
      661 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
      721 ataaaccgtt ataacactcc ctgttggcac gggaa
//
LOCUS       DACADA010000393          741 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.402, whole genome shotgun sequence.
ACCESSION   DACADA010000393 DACADA010000000
VERSION     DACADA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.402"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            22..594
                     /gene="hdeD"
                     /locus_tag="ISB17_004950"
     CDS             22..594
                     /gene="hdeD"
                     /locus_tag="ISB17_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAP1982765.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          160 a          152 c          170 g          259 t
ORIGIN      
        1 ttaattttat agggtggttc tatgttatat atagataagg caacaatttt gaagtttgat
       61 ctggagatgc ttaaaaaaca tcgcagagca atccagttta ttgccgtgct gctgtttatc
      121 gtcgggttgc tgtgtatcag ttttccgttc gtctctggcg acattttaag cacagtagtg
      181 ggtgcattat taatctgctc gggcattgcg cttattgtcg ggctattcag caaccgcagt
      241 cataatttct ggccggtatt atccggtttc ctcgtcgcag tcgcctattt attgatcggc
      301 tatttcttca tccgcgcacc agagctgggc atttttgcca ttgcggcatt tattgccggt
      361 ttgttctgtg tggcaggggt tattcgcctg atgagttggt atcgtcagcg ttcaatgaaa
      421 ggcagttggc tacagctcgt tattggtgtg ctggatatcg tcattgcctg gatattcctt
      481 ggtgctacgc cgatggtgtc ggtaacgctg gtttccacac tggtgggaat tgaactgata
      541 tttagcgccg ccagtctgtt cagcttcgcc agtttgttcg ttaaaaagca ataattatcc
      601 ctatttccac ctggatcata gtcacattaa gtgactatga tccagttaat actctcttag
      661 ctatacagtg tttcagttca attacgcact atcattgaaa caattgcctg ctgttgatta
      721 tttcaaccta ctgaatttaa t
//
LOCUS       DACADA010000394          741 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.403, whole genome shotgun sequence.
ACCESSION   DACADA010000394 DACADA010000000
VERSION     DACADA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.403"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            95..>741
                     /gene="acrS"
                     /locus_tag="ISB17_004951"
     CDS             95..>741
                     /gene="acrS"
                     /locus_tag="ISB17_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAP1982766.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTN"
BASE COUNT          223 a          150 c          166 g          202 t
ORIGIN      
        1 ttagcctctc gtataaatac acattaggtg atagattaac cttcgctatt ttctcatcct
       61 gtgtcgaata tatttatttc ctgaataatt aatcatggca aaaagaacca aagctgaagc
      121 cctgaagacc cggcaagaac tgattgaaac tgccatcgcc cagtttgcgc agcatggcgt
      181 aagcaagacg acgctcaacg acattgccga cgctgctaac gttacgcgtg gcgctatcta
      241 ctggcacttc gaaaacaaga ctcaactgtt taatgagatg tggttgcaac agccttcatt
      301 gcgggagtta atccaggatc acttgaccgc tgggttagag catgacccgt ttcagcaatt
      361 gcgtgaaaaa ttgattgtcg gtttgcaata tattgccaaa attccccgcc agcaggcgtt
      421 gctgaaaatc ttatatcaca aatgtgaatt taatgatgag atgctggccg agggagtgat
      481 acgcgaaaag atgggcttta atccgcagac tctccgcgaa gtattgcagg cgtgtcagca
      541 acaaggttgt gtagcaaata atctcgattt agatgttgtg atgattatta ttgatggtgc
      601 cttcagcgga attgttcaaa actggttaat gaatatggcg ggttatgatc tttataaaca
      661 agcccccgct ctggtcgata acgtattaag aatgttcatg ccagatgaaa acataacgaa
      721 attaattcat aaaacgaatg a
//
LOCUS       DACADA010000395          738 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.404, whole genome shotgun sequence.
ACCESSION   DACADA010000395 DACADA010000000
VERSION     DACADA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.404"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..606)
                     /locus_tag="ISB17_004952"
     CDS             complement(<1..606)
                     /locus_tag="ISB17_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HAP1982767.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSD"
BASE COUNT          207 a          162 c          141 g          228 t
ORIGIN      
        1 atcactataa cttgttgatg tatatgcctg gttattatta aaagcatcat cacttaaata
       61 gacgtcatta ttgatggtat aaattgcacc gcctttgccg tcattagcga tgttattgtg
      121 aaatacggcg ttagtgactc gtaaatcggc ggcaccggta tcattggtac cggcagaata
      181 gattgctccg ccatagcctc ctgcaacatt actgtcgaaa ataacattcg caagatttat
      241 cgttgagttt tctttagcaa agatagcccc gccattatta tattcgccac taaccgagtt
      301 atcagcaaac agggtcatcc ctgtttcatt ttgtggtgat atcgtaaatt cagcactgtg
      361 ctgcaaaaat actgcgccac cgctggcatt attgaccata cccgaaaaaa tcaaccattg
      421 tccatccgcg atgctccagt cctgtatatt acccgaaagg ctttgcctgc tggcctgaca
      481 gctcgctttg atatcatatc cctggcatga atcaatgacc gctgcggcac cgttagcaga
      541 gaaaagagat gcaatcattg acgggagtaa agataaatac tccttgcgta gagagataat
      601 ccgcatattc atctaaccat cattttctat aagatggcgt atttaatcgc attatgcgat
      661 atagggtatt tttcttttgt aaattctgcc cccgatgtaa taaccacttt gttattattt
      721 gatgattaat ttagatgc
//
LOCUS       DACADA010000396          731 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.405, whole genome shotgun sequence.
ACCESSION   DACADA010000396 DACADA010000000
VERSION     DACADA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.405"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            2..>731
                     /locus_tag="ISB17_004953"
     CDS             2..>731
                     /locus_tag="ISB17_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAP1982768.1"
                     /translation="MNALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEY
                     IKEVFISSGARVTAQDIPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSYENDQLTY
                     TPGADDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLE
                     RPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAA
                     LLSSRDLSVYSMNAPGFIPGIDFSD"
BASE COUNT          182 a          177 c          184 g          188 t
ORIGIN      
        1 ggtgaatgca ttaccaccaa cgccccgaca cgctagtcct gagcaattag aaaaaacagt
       61 tcgttatctt acacaaactg tgcatccacg tagcgccgac aatattgata atctgaatag
      121 atccgcagag tatataaaag aggtctttat cagtagcggt gccagagtta ccgcgcagga
      181 tatccccatt acgggtggcc cctacaaaaa cattgttgct aattatggtc ctgccgatgg
      241 accgctgatt attattggtg cgcactatga ctctgtcagt agttacgaaa acgatcaatt
      301 gacctatacg ccgggcgcgg atgataacgc cagcggtgtg gcaggattac tcgaactggc
      361 acgtttgtta cagcagcaag caccgaaaac aggcgtgcag ctggtcgcct atgcgtcgga
      421 agaaccgccc ttctttcgta gcgatgaaat ggggagcgcg gtacatgcag cttcgcttga
      481 gcgtccagtg aaattaatga tagcgctgga gatgattggt tattacgact ctacgcctgg
      541 tagccaggat tacccatccc cggccatgtc ctggctttat cccgatcggg gagactttat
      601 tgccgtggtc ggcagaatgc aggatatcaa cgccgttcgt caggtaaaag cggcattgtt
      661 gtcatcccgg gatttatctg tttattctat gaatgcccca ggttttattc ccggtattga
      721 tttctctgat c
//
LOCUS       DACADA010000397          730 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.406, whole genome shotgun sequence.
ACCESSION   DACADA010000397 DACADA010000000
VERSION     DACADA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.406"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>730)
                     /locus_tag="ISB17_004954"
     CDS             complement(<1..>730)
                     /locus_tag="ISB17_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982769.1"
                     /translation="LYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPTGIML
                     MLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGFMGDWL
                     GTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKIISGYFN
                     VEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLIGFDM
                     PSRVQRWIHPEAALEYDTVDGKPFY"
BASE COUNT          269 a          184 c          129 g          148 t
ORIGIN      
        1 cataaaaagg ttttccatcg acagtatcat attcaagcgc agcttctgga tggatccatc
       61 tctgtacacg tgatggcata tcaaaaccaa taaggacaag aacaacaaat gttaagataa
      121 aagcaagaga acacattgct gtaccaagac ttgtatgaga cgcaacaact gcaccaagaa
      181 taggcgcaca tgctccacca agtgaaccta cattataaat aaaccccagt cctgccgcac
      241 gctgctccac attaaaataa ccactgatta ttttcggcac cagagcacca atcccctgac
      301 caaaaacctg ctgagtaaag attagaaggc caagtaacca tacatagtcc cggtccacaa
      361 gaaaaacggg gataataaca agctgaccga taagaagtga tataacatat gcttttcggg
      421 ttccaagcca gtcccccata aaaccaccta tacaacttcc agctgcagag ccaaaacctg
      481 ccagcataaa tatgagagca acagtttctg gtgaatactg catatcaacc ttaagccatg
      541 taggaagaaa agcctgcagt ggccatccgt agaggaaaca gaacataacc acaagcatca
      601 acataatgcc tgttggccat cttttcccca taccctgata tataaaactg ataaaaacag
      661 ccgcacagca caatgccata actgtgatta aagcagcgtt tgcagtgacg cctgaaaaac
      721 aaacatatag
//
LOCUS       DACADA010000398          716 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.407, whole genome shotgun sequence.
ACCESSION   DACADA010000398 DACADA010000000
VERSION     DACADA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.407"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>716)
                     /locus_tag="ISB17_004955"
     CDS             complement(<1..>716)
                     /locus_tag="ISB17_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAP1982770.1"
                     /translation="KSEPPKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIARE
                     IRDQGYRGGMTILRGFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFV
                     AVLGYSRMLYIEFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTG
                     QHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGIT
                     VDVETANRHGLRWLHDVANQR"
BASE COUNT          171 a          188 c          197 g          160 t
ORIGIN      
        1 cgttggttag cgacatcgtg cagccagcgc agaccgtggc ggttggctgt ttcaacatcg
       61 acagtgatcc ccatcgggcg caggcgagtc attagtggga tgtaaaaact gttacgggtg
      121 tactgcacca tccgttccac cttaccttta gtctgtgccc tgaaggggcg acacagtcgg
      181 ggagagaagc ccatctcctt gccgaactgc cacagcgaag gatggaaccg gtgctgaccg
      241 gtctgatatg cgtcacgttg cagaaccaca gttttcatat tgtcatacaa cacttcgcgc
      301 ggcacaccac caaagaagcg gaacgcatta cgatggcagg tttccagcgt gtcataacgc
      361 atattgtcag tgaattcgat gtacaacatt cggctgtatc cgagaacagc aacgaacacg
      421 tgaagcggtg agcggccatt acgcatagtg ccccagtcaa cctgcatctg tcgtccgggt
      481 tcagtttcga accgaacggc aggctcctgc tcctgaggaa ccgagagaga acgaatgaat
      541 cccctgagaa tggtcattcc gccacgatat ccctggtctc tgatctcgcg agcgattacc
      601 gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt gacgaatata atcccggtat
      661 tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt ttggcggctc agattt
//
LOCUS       DACADA010000399          706 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.408, whole genome shotgun sequence.
ACCESSION   DACADA010000399 DACADA010000000
VERSION     DACADA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.408"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..423)
                     /locus_tag="ISB17_004956"
     CDS             complement(<1..423)
                     /locus_tag="ISB17_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982771.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKFTTYDFR"
BASE COUNT          214 a          133 c          152 g          207 t
ORIGIN      
        1 acggaaatca taagtggtaa atttttcgtt acataatatt ctgctatcgt attcactttt
       61 atcgtcatga gattttaggt aaacatcctt atgcaaccca tgatttttca caaataaaac
      121 cagattaaga ttcggctggt agtgattatg tgcttcatca taaccaaata aaagctcttc
      181 ccggcggcag agaaaacaag ctgtttttcc tggtgcgatt tttaatttga tgtggggacg
      241 tgggaaatta gtccaaagat tgccatcgtc atcacactgg ttaatgttaa agccataggg
      301 tgcgaaagaa gcaactgacg cagggtgtac actagtttta agctggtttt tgcggcgaat
      361 ccattcatta gcagacagaa attcccccgc catgatgaca gcggtatgaa tattatcctt
      421 catccattta tccgctaatt ctctggaatt aaatgtgggt cggttagcgg taaatgtaag
      481 cggaagtgag ggtagaggaa acggtacaat atcacctata tcatcatgcc atatatagtt
      541 tggccattgg tttcctggtt gtaggtgata acgcagggct tttgacccac cggtataaaa
      601 atgggcatct gctggtaatg tagaacgaac aattccctta cccttaaagg caacttctcg
      661 cacaaaaaat tctaaaaata ataccggcca aggaagagta agtttg
//
LOCUS       DACADA010000400          698 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.409, whole genome shotgun sequence.
ACCESSION   DACADA010000400 DACADA010000000
VERSION     DACADA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.409"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>698
                     /gene="tuf"
                     /locus_tag="ISB17_004957"
     CDS             <1..>698
                     /gene="tuf"
                     /locus_tag="ISB17_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1982772.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTG"
BASE COUNT          162 a          170 c          199 g          167 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactgg
//
LOCUS       DACADA010000401          689 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.410, whole genome shotgun sequence.
ACCESSION   DACADA010000401 DACADA010000000
VERSION     DACADA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.410"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            287..550
                     /locus_tag="ISB17_004958"
     CDS             287..550
                     /locus_tag="ISB17_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP1982773.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            550..>689
                     /gene="rpmJ"
                     /locus_tag="ISB17_004959"
     CDS             550..>689
                     /gene="rpmJ"
                     /locus_tag="ISB17_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP1982774.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
BASE COUNT          209 a          149 c          159 g          172 t
ORIGIN      
        1 gtgggagagg aatacacatt gtcaggtaat caatcatgct gcaataaatc atcggccagt
       61 aaagtggaga tagcctccat tctcgaaaaa tccatactct cagcgaaacc atcatcaatc
      121 actcatccag gcgtttatgg gagcgtcgcc aatggctgct aacaatgcca gacttccccg
      181 ttgcggaaat tccacatccc acaaatagtc acagtgattg ggtgttgaaa tgatccggat
      241 gagcatgtat ctttacggtt atgttataac ataacaggta aaaatgatga aacccaatat
      301 ccatcctgag tatcgtactg tggtgttcca cgacaccagc gttgatgagt actttaaaat
      361 cggctcgact atcaaaacag accgtgagat tgagctggat ggcgtaacgt atccgtacgt
      421 aacaattgat gtctcttcga aatcgcaccc gttctataca gggaagctga gaacagtggc
      481 atcagaagga aatgttgcac ggttcaccca acgttttggt cgttttgtta gcacgaaaaa
      541 gggggcatga tgaaagtcct taactctctg cgtaccgcaa aagaacgcca tccagactgt
      601 cagattgtga agcgaaaagg acggctgtat gtgatttgta aatctaatcc acgttttaag
      661 gccgttcagg gtcgtaagaa aaaacgttg
//
LOCUS       DACADA010000402          689 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.411, whole genome shotgun sequence.
ACCESSION   DACADA010000402 DACADA010000000
VERSION     DACADA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.411"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            50..646
                     /gene="fimE"
                     /locus_tag="ISB17_004960"
     CDS             50..646
                     /gene="fimE"
                     /locus_tag="ISB17_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAP1982775.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          193 a          133 c          168 g          195 t
ORIGIN      
        1 ttcctatagg tattcattca aatatatctc agttaggagt actactattg tgagtaaacg
       61 tcgttatctt accggtaaag aagttcaggc catgatgcag gcggtttgtt acggggcaac
      121 gggagccaga gattattgtc ttattctgtt ggcatatcgg catgggatgc gtattagtga
      181 actgcttgat ctgcattatc aggaccttga ccttaatgaa ggtagaataa atattcgccg
      241 actgaagaac ggattttcta ccgttcaccc gttacgtttt gatgagcgtg aagccgtgga
      301 acgctggacc caggaacgtg ctaactggaa aggcgctgac cggactgacg ctatatttat
      361 ttctcgccgc gggagtcggc tttctcgcca gcaggcctat cgcattattc gcgatgccgg
      421 tattgaagct ggaaccgtaa cgcagactca tcctcatatg ttaaggcatg cttgcggtta
      481 tgaactggcg gagcgtggtg cagatactcg tttaattcag gattatctcg ggcatcgaaa
      541 tattcgccat actgtgcgtt ataccgccag taatgctgct cgttttgccg gattatggga
      601 aagaaataat ctcataaacg aaaaattaaa aagagaagag gtttgattta acttattgat
      661 aataaagtta aaaaacaaat aaatacaag
//
LOCUS       DACADA010000403          682 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.412, whole genome shotgun sequence.
ACCESSION   DACADA010000403 DACADA010000000
VERSION     DACADA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.412"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            71..>682
                     /gene="waaH"
                     /locus_tag="ISB17_004961"
     CDS             71..>682
                     /gene="waaH"
                     /locus_tag="ISB17_004961"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAP1982776.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALR"
BASE COUNT          179 a          142 c          169 g          192 t
ORIGIN      
        1 acaggcttaa tagtttctta atacaaagcc tgtaaaacgt caggataact tcagaggtcg
       61 tcggtaattt atgatgaaca gcaccaataa acttagtgtt attatcccgt tatataatgc
      121 gggcgatgat ttccgcactt gtatggaatc tttaattacg caaacatgga ctgctctgga
      181 aatcattatt attaacgatg gttcaacgga taattctgtt gaaatagcaa agcattacgc
      241 agaaaactat ccgcacgttc gtttgttgca tcaggcgaat gctggcgcat cggtggcgcg
      301 taatcgtggg attgaagtgg caacgggaaa atatgtcgct tttgtcgatg ctgacgatga
      361 ggtctatccc accatgtacg aaacgctgat gaccatggcg ttagaggatg acctcgacgt
      421 ggcgcagtgc aacgctgatt ggtgttttcg tgaaacgggt gaaacctggc aatccatccc
      481 caccgatcgc cttcgctcaa ccggtgtatt aaccggtccg gactggctgc ggatgggact
      541 ttcttcccgc cgttggacac acgtggtctg gatgggggtt tatcgccgcg atgttattgt
      601 taaaaataac attaaattta ttgccggatt acatcatcag gatattgtct ggacaacaga
      661 atttatgttt aacgcgctgc gt
//
LOCUS       DACADA010000404          673 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.413, whole genome shotgun sequence.
ACCESSION   DACADA010000404 DACADA010000000
VERSION     DACADA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.413"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            60..>673
                     /locus_tag="ISB17_004962"
     CDS             60..>673
                     /locus_tag="ISB17_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAP1982777.1"
                     /translation="MAYGEGIPLPDTLDKPESRVKQLARRKTITPGGKECKFNEIIPA
                     DHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLLPYIPEQAGILLVPCCRGGA
                     AFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKVALEYNRKNKLLSVCWMQGE
                     FDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSERVPWL"
BASE COUNT          189 a          127 c          175 g          182 t
ORIGIN      
        1 attttaatga aaaaataggt ttctggtatg tgatagccct cgcaggacag tctaatggta
       61 tggcctacgg tgaaggtatt cccctgccag atacactcga caaacccgag tcccgtgtaa
      121 aacaactggc tcgtcgaaaa acaatcacac caggaggaaa agagtgtaaa ttcaatgaga
      181 ttattccggc agatcactgc ctgcatgatg ttcaggatat gagtggatat caccatccgg
      241 ctgctgattt gcataaaggg gaatatggtt gtgtcggaca gggattgcat atagctaaaa
      301 agttactgcc atacatacct gagcaagcag ggatattact ggttccttgt tgcagaggag
      361 gtgccgcctt tactgttgga gctgaaggta tgtatgtacc tgatacaggg gcaacagctg
      421 atgcaatgcg gtggggcacc ggaacagcac tctatgagga cttagtagcc cgggttaagg
      481 tagctctgga atataacaga aagaataaac tgctatctgt ctgctggatg cagggggagt
      541 ttgatcttat gtcaccagat tatgaaaagc atcctgactt gttttatcag atggtaactt
      601 ctttcaggtc tgagttatct gaatactcat ctcagtgtgt tggtaattcg tcagaaagag
      661 ttccatggtt gtg
//
LOCUS       DACADA010000405          669 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.414, whole genome shotgun sequence.
ACCESSION   DACADA010000405 DACADA010000000
VERSION     DACADA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.414"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            145..507
                     /locus_tag="ISB17_004963"
     CDS             145..507
                     /locus_tag="ISB17_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAP1982778.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          200 a          155 c          152 g          162 t
ORIGIN      
        1 tttcttaact aatcaatgta ttgggtgatt ttttaacgca ttaatggtta caatctggaa
       61 ctattacaat gccatcagtt gtgaacaaag cacctggtcg cgcatactag aggctataaa
      121 tttatctcta tcagaagtca tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc
      181 agcgatcgtc tggacgccat ccgtcaccag caggccgatc tatcgctggt tgaagccgca
      241 gacaaatatg ccgaactgga aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg
      301 cgcgaagttc acagccagaa actcagcaaa gaagcgcaga aactgatgaa gatgccgttc
      361 cagcgtgcaa tcaccaaaaa agagcaggct gatatgggca aactgaaaaa aagtgttcgc
      421 ggactggtgg ttgtgcaccc aatgaccgcg ctgggccgtg aaatgggcct gcaagagatg
      481 acagggttct caaagactgc gttttaagtt cctttctcgt cgctgtaact actgccactc
      541 acgatttact cgctcctgct ggcacatgca ggagcctgtg ccactcatta aatacgatgt
      601 ctttcttacc gtaaactgtt tcagtttccc ctcgagtttg aggtattttt gcaggctaaa
      661 atctgaaaa
//
LOCUS       DACADA010000406          664 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.415, whole genome shotgun sequence.
ACCESSION   DACADA010000406 DACADA010000000
VERSION     DACADA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.415"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>664
                     /locus_tag="ISB17_004964"
     CDS             <1..>664
                     /locus_tag="ISB17_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1982779.1"
                     /translation="IVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKFVI
                     ERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTIAN
                     VRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEEGN
                     IVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALEDD
                     DDV"
BASE COUNT          220 a          116 c          146 g          182 t
ORIGIN      
        1 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
       61 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
      121 aaattcgtta ttgaacgtgc aaaacaagca caagttccaa agcacgttat tgataaagca
      181 attgataaag ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt
      241 ggtcctaatg gctcaatgat tatcgctgag acattaactt caaatgttaa ccgtacgatt
      301 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
      361 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
      421 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
      481 attgttattt atactgaacc tactgacctt cataaaggaa tcgcggcgct aaaagcagct
      541 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
      601 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
      661 gtac
//
LOCUS       DACADA010000407          661 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.416, whole genome shotgun sequence.
ACCESSION   DACADA010000407 DACADA010000000
VERSION     DACADA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.416"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            555..>661
                     /locus_tag="ISB17_004965"
     CDS             555..>661
                     /locus_tag="ISB17_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HAP1982780.1"
                     /translation="MGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAKL"
BASE COUNT          225 a          117 c          145 g          174 t
ORIGIN      
        1 ctgataaaga tctcatcagg aagaatgagt tattagcctt atccctgaag gaattgtatc
       61 aacttctcgt ttcaaaaaaa taacaagtaa tacggcgagc gatatgacgt gccatgggat
      121 atccgcaccc agcagcatgt tgtctccacg atgtccgcag ttcgctcata atagacattc
      181 actacagtta tagtagaagg tatgcatgct gctgagtggg gaaagtgtga aagaaaagaa
      241 gacagctgct ttgtttgtcg tcacgtttat cttcattggc tatgcaagtc gtaatacaag
      301 gcgggacaaa actgagacac ataaggcctc gcaatggctt gcaaggccac acatgtattg
      361 agatgttaat aaaatgtaga cttgtaattt tgatataaat ggtagagaaa atctttcccc
      421 aaaataaaaa cgaacgtcaa tgaaatcaaa cggttgaata aagttgattt tggctaatac
      481 aaagacaaga aaataatatt tatgattaaa tatcagcgag ttgaatacat aatttttata
      541 tactgctgcg ccacatgggt tggactgaag ccgcagacct gattgttaaa ggtatggaag
      601 gcgcaatcaa tgcgaagacc gtaacctatg acttcgaacg tctgatggaa ggcgctaaac
      661 t
//
LOCUS       DACADA010000408          655 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.417, whole genome shotgun sequence.
ACCESSION   DACADA010000408 DACADA010000000
VERSION     DACADA010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.417"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>655)
                     /locus_tag="ISB17_004966"
     CDS             complement(<1..>655)
                     /locus_tag="ISB17_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1982781.1"
                     /translation="TVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTA
                     FILSYRMPSVNGYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHL
                     HYEEETSVRPDWMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPV
                     FIVHATDDKDVPVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSL
                     "
BASE COUNT          208 a          167 c          104 g          176 t
ORIGIN      
        1 aggctttgcc atttttctat cggatatcca ttttttccgg atacaccaaa tccatgacct
       61 cccttatcaa aaaaatgagc ttctgcatga tgtcccttat cccgaataga gttaaaatat
      121 ctcagagaat gaataactgg aacatcctta tcatctgttg catgcacaat aaagactggt
      181 ggcatatact ctgtaacact attttcaaga ctggctgaat attcactttc acctgcatcc
      241 agaagacgtt tccggcatcc ctgatgagca atactcctgt caagagatat aactggatat
      301 atcagagcca tccagtcagg acgcacagaa gtctcttcct cataatggag atgagtaata
      361 tatccagcaa gatgaccacc tgccgaaaac cccatacatc caacactctt aaatgttctc
      421 tccgacattc cggcccagta agaaataaag ttcagggcat tcttcgcatc tgcaaaggaa
      481 taataaccat taacggaggg cattctgtaa gaaagaataa atgccgtata tccctgacct
      541 gctatccatc tgccaacatc aagtccctct ctttcataaa caatcctcct gtatccacct
      601 ccaggtataa tcagcacact atgttgatta gatacaacag gctcaacaac tgtaa
//
LOCUS       DACADA010000409          652 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.418, whole genome shotgun sequence.
ACCESSION   DACADA010000409 DACADA010000000
VERSION     DACADA010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.418"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>652)
                     /locus_tag="ISB17_004967"
     CDS             complement(<1..>652)
                     /locus_tag="ISB17_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982782.1"
                     /translation="KQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQHEF
                     GLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLAPGY
                     AVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGLAAQVYSI
                     VVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDILYREKNK"
BASE COUNT          215 a          168 c          114 g          155 t
ORIGIN      
        1 tttattcttt tctctgtaca gaatatccac cattgttctg acgggtttat tctcttgttg
       61 ccttttttgc cagtcatcag actccggaag attcttgcga agataaaaag caaataatat
      121 tggaagcatg ccgacaaaga aaagagaacg ccagccccat agtggtacta caatgctata
      181 aacctgtgca gctaaacctc ccccaataga aaatccagaa attaaaaaac cacttgcctt
      241 gtttctgaga tgaacaggcc agctttcgat aacatatgtt acacttgagc catattcacc
      301 tgccatacca agcccaataa caatgcgagc aataaacatc actgcatatc caggagccag
      361 accacaggca agggttccga gagagaatag cactatactt aaaaccattg ccatcctgcg
      421 ccccatcttg tccgataaag cgccaatagc aagcccccca aaccatcgag aaataaaagc
      481 agcggaaata aggcttgccg cttctattgt cgtcaagccg aattcatgct gaacctctgt
      541 aagaaccaga gatatcagaa cgaaatcaaa accatcaagt aaataacctg tccatgccgc
      601 accgaatatc ttccactgtc tgggtgaaag atgcttatac caaacctgct tt
//
LOCUS       DACADA010000410          651 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.419, whole genome shotgun sequence.
ACCESSION   DACADA010000410 DACADA010000000
VERSION     DACADA010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.419"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>651
                     /locus_tag="ISB17_004968"
     CDS             <1..>651
                     /locus_tag="ISB17_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1982783.1"
                     /translation="YITPSAVSQSLQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLE
                     KNLRGLEQTINIVNKSELKKNFIIYGPQLISCTNNNMLIRCLRQDTSIDIECHDILLS
                     AENAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNYEQII
                     AEEFTLLISKSAGIDEIQMDIDERFINRKVSFRSSSLLTIINSISVTDLLGIVPTEL"
BASE COUNT          204 a          145 c          108 g          194 t
ORIGIN      
        1 tatataacac cctctgctgt tagtcagtcg ttacaacgtt tacgtgcgca atttaatgac
       61 ccgttattca ttcgctcagg gaaaggtatc gcgccgacta caacagggtt aaacctgcat
      121 caccatcttg agaaaaatct cagaggcctt gaacaaacga tcaatatcgt taataagtct
      181 gaactaaaga aaaattttat tatctacggt cctcagctta tttcttgtac taataacaac
      241 atgctaatcc gctgtcttag acaagatact tcaatcgata tagaatgcca tgacatactg
      301 ctttcagctg aaaatgccga agaactgttg gttcaacgca aaactgacct ggcaattacg
      361 ctgcaaccag taattagtcg ctctgtcatc tgcatgccgt tacacactat tcgcaattcc
      421 cttatatgta gcaacaaaca tcctcgtatt actgatacca gtaactatga acaaattatc
      481 gccgaagaat tcaccctgtt gatatctaaa tcagcaggga ttgacgaaat tcaaatggac
      541 atagatgaac gttttataaa ccgtaaagtt tcatttcgca gttcttcttt gttgactata
      601 attaatagca tttctgttac tgacttatta ggcattgtgc ccaccgaact g
//
LOCUS       DACADA010000411          649 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.420, whole genome shotgun sequence.
ACCESSION   DACADA010000411 DACADA010000000
VERSION     DACADA010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.420"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            3..>649
                     /locus_tag="ISB17_004969"
     CDS             3..>649
                     /locus_tag="ISB17_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982784.1"
                     /translation="MASIKNEHVSVLDSYKVRSFKETHSDTACIVRIIEIYSLNKLRA
                     KGEKLYSLTGLTVPDTETVANEINLLLSRYAQLCRQEEEELSFRQREVTNAEVAWKST
                     FSKNGVSSIAEAKTNKMGHAERADAERYYHLAVSRLNEQHSRLSTIKLLPGVLADEGN
                     YIGKGIDKRLLNIFPQSGQIPADFISVFNDSDVVRDIKFITDALKSLSDSVSEII"
BASE COUNT          196 a          117 c          158 g          178 t
ORIGIN      
        1 aattggcatc aattaagaac gaacatgtgt cggtgttaga tagttacaaa gtaagatcat
       61 ttaaagagac tcatagcgac acggcctgta ttgtgcgtat tattgaaata tattcactga
      121 ataagctgcg agctaaaggt gaaaagctgt attcacttac ggggttaaca gtacctgata
      181 cggaaactgt ggcgaatgag atcaacctgc tcctgagccg ttatgcccag ttgtgtcgtc
      241 aggaagagga agagttatct ttccggcagc gtgaagtgac aaatgcggaa gttgcatgga
      301 aaagcacatt ctcaaaaaac ggcgtcagta gcattgctga agccaaaacg aataaaatgg
      361 ggcatgctga acgagcggat gctgaacggt attaccatct ggctgtttcc cggttgaatg
      421 aacaacatag tcgattgagc acgatcaagc ttttgccggg agtactggcc gatgaaggaa
      481 actacattgg gaagggaatc gataagcgtt tactgaatat atttcctcaa tccggccaga
      541 tccctgctga ttttatttct gtatttaatg acagtgacgt tgtccgtgat attaaattta
      601 ttactgatgc actcaagtct ttatctgatt ccgtgagtga aattattag
//
LOCUS       DACADA010000412          644 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.421, whole genome shotgun sequence.
ACCESSION   DACADA010000412 DACADA010000000
VERSION     DACADA010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.421"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..461
                     /locus_tag="ISB17_004970"
     CDS             <1..461
                     /locus_tag="ISB17_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982785.1"
                     /translation="QMVVSAIASTPQTEFSWFRYGQYNETGIYISIEKTIIITITVSG
                     GCFKKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMT
                     LRLRLQDQQEETSVTLFQGKISTDGNNYSIIKGKVSSVLLLLKMLQINHA"
BASE COUNT          190 a          162 c          123 g          169 t
ORIGIN      
        1 aacaaatggt tgtgtccgct attgccagta cacctcaaac tgagttctca tggttccgat
       61 acggacagta taacgaaacg ggtatttaca tctcgatcga gaagaccatt atcattacaa
      121 tcaccgtttc tggtgggtgc tttaaaaaag aatacagcat tgtctcccac atgctggtca
      181 ctgacaccat aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac
      241 tcagtcgccc agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa
      301 atatgacctt acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc
      361 agggaaaaat aagtaccgac ggaaataact attccatcat caaaggtaaa gtaagctcag
      421 tacttctgtt attaaaaatg ctgcagatta atcatgcctg atctctgtcc cggtatttta
      481 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccagtca
      541 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      601 ttcataagcg tattatcagg attgtctgga aaattcccga tact
//
LOCUS       DACADA010000413          633 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.422, whole genome shotgun sequence.
ACCESSION   DACADA010000413 DACADA010000000
VERSION     DACADA010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.422"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>633
                     /locus_tag="ISB17_004971"
     CDS             <1..>633
                     /locus_tag="ISB17_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982786.1"
                     /translation="TDAFPTYIPISSTKKFCDEFDIMIGRYGPPIFQICRGLKGAYNV
                     ALLKVIPKEGVSRDFLYYFLKQDSVFQYVDKLSARTGGQTGVDLVSLKEYPVRIPEEI
                     ECQEKLVTILSVIDKKIALNNRINTELEAMAKTLYDYWFVQFDFPDANGKPYKTSGGK
                     MEYNATLKREIPAGWNDSILGKFIELDRGVTYSKEDVRTQDDKDTIGILRA"
BASE COUNT          199 a          110 c          142 g          182 t
ORIGIN      
        1 aacggatgca tttcccacgt atataccaat atcgtcgacg aagaaatttt gtgatgaatt
       61 cgatattatg attggtaggt atggtccccc aatttttcaa atatgtcgcg gattgaaagg
      121 agcatataac gttgccttgc tgaaggttat acctaaagaa ggtgtaagtc gtgatttttt
      181 atactatttt ttgaagcaag actctgtttt ccaatatgtt gataaattat ctgccagaac
      241 cggaggacaa actggagttg atctagtttc tttaaaagaa tatccagtca ggatacctga
      301 agaaattgaa tgtcaagaaa aattggtgac tattcttagt gttattgata aaaaaatcgc
      361 cctcaacaac cgcatcaaca cggaactgga agcgatggcg aaaacactgt atgactactg
      421 gtttgtacag tttgatttcc ctgatgccaa cggaaaaccg tataaaacct ccggcgggaa
      481 gatggagtat aacgccacgc tgaaacggga aattccggca ggttggaatg actccattct
      541 tggtaagttt attgagttag atcgtggcgt tacttatagc aaagaggatg ttcgaactca
      601 ggatgataaa gatacgatag gcattcttcg cgc
//
LOCUS       DACADA010000414          633 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.423, whole genome shotgun sequence.
ACCESSION   DACADA010000414 DACADA010000000
VERSION     DACADA010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.423"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>633
                     /locus_tag="ISB17_004972"
     CDS             <1..>633
                     /locus_tag="ISB17_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1982787.1"
                     /translation="AVSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDISNVWARD
                     YLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIGGSYSVTV
                     AHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAETNPTTYSD
                     ALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASL"
BASE COUNT          202 a          124 c          129 g          178 t
ORIGIN      
        1 tgctgtttct gaattagcga aaagagtttc tggtaaaaca aaccgaaaac ttgtagcaac
       61 aatgttgtct ctggctgttg ccggtacagt aaatgcagca aatattgata tatcaaatgt
      121 atgggcgaga gactatcttg atcttgcaca aaataaaggt attttccagc ccggagcaac
      181 agacgtaaca atcactttaa aaaacggaga taaattctct ttccataatc tctcaattcc
      241 ggatttttct ggtgcagcag cgagtggcgc agctaccgca ataggaggtt cttatagtgt
      301 tactgttgca cataacaaaa agaaccctca ggccgcagaa actcaggttt acgctcagtc
      361 ttcttacaag gttgttgaca gaagaaattc caatgatttt gagattcaga ggttaaataa
      421 atttgttgtg gaaacagtag gtgccacccc ggcagagacc aaccctacaa catattctga
      481 tgcattagaa cgctacggta tagtcacttc tgacggttca aaaaaaatca taggttttcg
      541 tgctggctct ggaggaacat catttattaa tggtgaatcc aaaatctcaa caaattcagc
      601 atatagccat gatctgttaa gtgctagtct att
//
LOCUS       DACADA010000415          629 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.424, whole genome shotgun sequence.
ACCESSION   DACADA010000415 DACADA010000000
VERSION     DACADA010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.424"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..363)
                     /gene="traM"
                     /locus_tag="ISB17_004973"
     CDS             complement(<1..363)
                     /gene="traM"
                     /locus_tag="ISB17_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAP1982788.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFF"
BASE COUNT          199 a          130 c           86 g          214 t
ORIGIN      
        1 aaagaacctc tccatctcag atgacacctt ctccctgata tcttcgacca tattcgcata
       61 ttcaaacttt ggatttccgg agacatgagg actgagagac tcaataccca aaattttcgc
      121 tactgatgat tgtgttttta caacgcattc aagaagcaat ttattaaact cagtctgatt
      181 aaatgcagac tctttacgct ccatctgagt ctcatataca cgaagcccta attcgagaag
      241 catagtggat atgcttgaga aacttacatc tgtactttta gctccttctg cacgtcttct
      301 ttccacaatt ttatttattt tatagacaat ttcgtcactg acataagcct gcactttcgc
      361 catatgtata cctctttatt ttcttatcgc atcaaattta tttcacatat aaaaaaaaga
      421 caattcatcg atgaatcgaa tcgtgacgca agttacgaaa attacgattc atgcagcaaa
      481 tcacatcacg aattgaatct agagtcaatt cgctttaact cgttgtttta ttattactga
      541 ttcatctatg agttgtgtta aattcattgg tgaatcatat gcgattcacc aatgaatcct
      601 ttttataatg taaaataaat taaaataca
//
LOCUS       DACADA010000416          628 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.425, whole genome shotgun sequence.
ACCESSION   DACADA010000416 DACADA010000000
VERSION     DACADA010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.425"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(15..578)
                     /locus_tag="ISB17_004974"
     CDS             complement(15..578)
                     /locus_tag="ISB17_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1982789.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          193 a          136 c          128 g          171 t
ORIGIN      
        1 cagaaggtac tgttttaacg aggctttttt ttccacaccc ctttaaaacg agcagcatta
       61 ctggctgtgt atctgacagt atgctgaata tttcgatgac ccagataatc ctgaagtagt
      121 cgggtatcga caccattgtc agccagagca aaaccacagg catgacgtaa catatgagga
      181 tgtgctttta cagctaatcc tgcacgtttt ccagcctcac gaatgataga aaaaaaatgt
      241 tgtctgctga tagggcggcg tttccgggag agaaaaagcc agtctccttc aactccgttt
      301 tcaattaact tcctctgctt aagccacaac ttgatcaaat tatattcatc aggaagaaga
      361 ggatgtgttg tcgagaaacc atttttaata cgccgaatat taagttgttt tccagatgca
      421 tcaatatcgg ataaacgtaa atcaagaagt tcactggccc taaaaccgtg aatgaaagcc
      481 atcataatta gacagcgatt tctttcagga aaggacatgc tctgggctgc atccatcagc
      541 ctgtagactt catcctgtgt gagatatttt ctcgtcatac gccaccgtgt acagttgtaa
      601 aaaaaagaga ggcacaccca gataagta
//
LOCUS       DACADA010000417          627 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.426, whole genome shotgun sequence.
ACCESSION   DACADA010000417 DACADA010000000
VERSION     DACADA010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.426"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..626)
                     /locus_tag="ISB17_004975"
     CDS             complement(<1..626)
                     /locus_tag="ISB17_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP1982790.1"
                     /translation="MAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDI
                     ERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIV
                     VYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCISF
                     TLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLFLKQGHEI"
BASE COUNT          192 a          135 c          114 g          186 t
ORIGIN      
        1 atgatctcat gaccttgctt taaaaataga acttcatcat tagcgctgat gtgatcaaga
       61 tactcgttca gatatcgcca gtttaaatcg acatgaattt tatctaccgc taacaaatca
      121 tgatctttgc cgttgatgtg tagcgtaaaa gagatacatt ttttacccgg acgatcaaag
      181 caattgtatg ccgggttcca gctgttgtta gagtcatctg aagccatcga ccacggtcgt
      241 ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag cggtccattt
      301 acttgttcct gatgataaac aacgatttta tcattcgccc tccgcgaaaa gagataatat
      361 ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc cattttcaga
      421 atcacggcac gttgaatctc ctctgtattt tttaataaat acagtgagtc ctttaatgtc
      481 agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat aagttcaaag
      541 cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa
      601 agaccaataa atgacaatgc tgccaat
//
LOCUS       DACADA010000418          618 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.427, whole genome shotgun sequence.
ACCESSION   DACADA010000418 DACADA010000000
VERSION     DACADA010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.427"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(107..420)
                     /locus_tag="ISB17_004976"
                     /pseudo
     CDS             complement(107..420)
                     /locus_tag="ISB17_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          192 a          143 c          104 g          179 t
ORIGIN      
        1 cgtattttca gggcaggtta tttatcgaca gaatactgat ataacctgtc tgctttctga
       61 caagggcttc tttttttatc ttccagaaac tccggaatgc cataatttag tcaaatgccg
      121 atgactcatc tgtataataa ggtgcaagcc ttgctgcaag agcattcagc cgtataagtc
      181 tccccagtgt tgtactgaaa tacccattat tcatctggta tttctcgcag acctccttac
      241 gggagtgccc acctaccaga tagtccttca tagccagaat gaccctgtca ctgtgaatag
      301 aagaaatacc tatcagtaaa aaaaatgcat ttcagacata gagccgagca acagcacgct
      361 ctcgcgtata ttcagcaaaa acgcatttcc tgaccgactg atgacttcat gatgcgccat
      421 gtttccccct tctgtcgggc ccctggatat atgcttccgg cccggaaata ctcttcacgc
      481 aataagttaa atttagtttt ttatgttgta aatattgatt taaacgatct tttaacccac
      541 aaaacaaaat taaaccagaa taaaattcta acaacaagaa tacactcaga taaaaacatc
      601 atggtgaatt gaatttac
//
LOCUS       DACADA010000419          614 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.428, whole genome shotgun sequence.
ACCESSION   DACADA010000419 DACADA010000000
VERSION     DACADA010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.428"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            52..>614
                     /locus_tag="ISB17_004977"
     CDS             52..>614
                     /locus_tag="ISB17_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1982791.1"
                     /translation="MSKKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKS
                     ATYDFKASYVRADASLAATAGYVKTNTA"
BASE COUNT          178 a          120 c          139 g          177 t
ORIGIN      
        1 tgaaagggcc caattgtagt taaactcatg gaatatgatt caaggatttt tatgtctaaa
       61 aaattaggtt ttgcgctctc tggtcttatg ctggcaatgg ttgcaggtag tgcttctgca
      121 gatatggacg gtggtcagtt aaatatcagt ggtctggttg ttgataatac ctgtgaaacc
      181 cgcgtagacg gcggtaataa agatggcctg atcctgctgc aaactgcaac cgttggagaa
      241 atctctgagg gtgtgctgga cactaccgtt ggtgctaagg ctaaaccatt cagcattact
      301 gttgactgta gtaaagctaa tccggcacca ggcagcacag caaaaatgac ttttggttca
      361 gtcttctttg gtaacagtaa aggaaccctg aataatgaca tgtcaattaa tactccatct
      421 gacggtgtta atattgctct gcacaatatt gaaggttcta ctattaagca ggttaaaatc
      481 aacaatcctg gcgatatcta ttccaaagca ctggatgcaa cttccaaatc cgcaacttat
      541 gattttaaag cgtcttatgt tcgtgcagat gcaagtctgg cagccacagc aggttatgta
      601 aaaactaaca ctgc
//
LOCUS       DACADA010000420          609 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.429, whole genome shotgun sequence.
ACCESSION   DACADA010000420 DACADA010000000
VERSION     DACADA010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.429"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..347)
                     /locus_tag="ISB17_004978"
     CDS             complement(<1..347)
                     /locus_tag="ISB17_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1982792.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVA"
BASE COUNT          163 a          155 c          130 g          161 t
ORIGIN      
        1 tgtgccacag ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc
       61 ccgtcatcaa tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc
      121 atatcggcag tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc
      181 tccaccttca tcctcagcaa atccagccct tcccgatctg ttgaactgcc ggatgcctta
      241 tcggtaaata tacggtttgc tttcacacct gcgtctttga gtgctctgac ctgaagatca
      301 agagactgct gactggttga gacccgagcg taaccaaaaa gtcgcataaa aatgtacctt
      361 aaatcgaata tcggacaact catgtctatt attacaaatt tacgatttaa tagacatatt
      421 aatgtaacag ttttacgatg tccgataatt tataacattt cgtacggttg gaaaaatgtt
      481 actaaatgcc cgtcaggcag ggaggccgat atgcccgttg actttctgac cactgagcag
      541 actgaaagct atggcagatt caccggtgaa ccggatgagc ttcagctggc acgatatttg
      601 gcactgttg
//
LOCUS       DACADA010000421          605 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.430, whole genome shotgun sequence.
ACCESSION   DACADA010000421 DACADA010000000
VERSION     DACADA010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.430"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            65..605
                     /gene="papA"
                     /locus_tag="ISB17_004979"
                     /pseudo
     CDS             65..605
                     /gene="papA"
                     /locus_tag="ISB17_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
BASE COUNT          160 a          102 c          163 g          180 t
ORIGIN      
        1 tcgggtcatt ttaaattgcc agatatctct ggtgtgttca gtaatgaaaa agaggttgtt
       61 atttatgatt aagtcggtta ttgccggtgc ggtagctatg gcagtggtgt cttttggtgt
      121 aaatgctgct ctaactactc cacaggggca gggtagagta acctttaacg gaactgttgt
      181 tgatgctcca tgcagcattt ctcagaaatc agctgatcag tctattgatt ttggacagct
      241 ttcaaaaagc ttccttcaag caggaggcgt atccaaacca atgaatttag atattgaatt
      301 ggttaattgt gatatcactt catttaaggg gggggaggag tctcggcagc aaaaaaaggg
      361 actgtgaagc tggcttttag tggtccaagg gtttctggtc ataatgagga gttagatacc
      421 agcgggggga caggtactgc gattgtagtt caggctgcag gtaaaaacgt ttctttcgat
      481 ggcacagaag gtgatgctaa taccctgaaa gatggtgata atgtgctgca ttatactgcg
      541 attgttaaga agtcgtcagc caataatgcc caagtcactg agggtgcttt ttctgcagtc
      601 gcaac
//
LOCUS       DACADA010000422          603 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.431, whole genome shotgun sequence.
ACCESSION   DACADA010000422 DACADA010000000
VERSION     DACADA010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.431"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            11..250
                     /locus_tag="ISB17_004980"
     CDS             11..250
                     /locus_tag="ISB17_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982793.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGTKEIVKKFH
                     IYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            251..541
                     /locus_tag="ISB17_004981"
     CDS             251..541
                     /locus_tag="ISB17_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP1982794.1"
                     /translation="MYNFKDKIEDYTEREFIELLGEFTNPTGDNAQLKGEVLDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKDSE"
BASE COUNT          210 a           98 c          132 g          163 t
ORIGIN      
        1 ttggctggag gtgagtcagt atccggagtt gatagcgcag ttttcaagcg gtaatcaaac
       61 acgtataaaa caaggattaa tcgcaaaagc accacttgaa ggttggcatt atggcactaa
      121 agaaatcgtt aaaaaattcc acatatatca tcgtgttgcg attgaatata gtggcggaat
      181 ttacgatatt gataatttaa ggagggcaca cctcatttgc atgatgaaat ttattacagg
      241 agatagttaa atgtataatt ttaaagataa aatagaagat tacacagagc gggaattcat
      301 tgagttgctt ggcgagttta caaatccaac cggggataac gctcaattaa aaggggaagt
      361 gcttgacaaa tattgggatg atctagaaga acatcttacc agaattacac aacatccgtt
      421 gatgagcgat cttatctact atcctgcaaa gaagggagat gatgaacctg aaaacatcct
      481 gaaaatcgtc aaagaatggc ggcgttcgca aggattgcct ttgttcaaag attcagaatg
      541 aactctaaac agaatctgtc acctgaaaat catcacgttc tggttaagtg atgattttcg
      601 cta
//
LOCUS       DACADA010000423          587 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.432, whole genome shotgun sequence.
ACCESSION   DACADA010000423 DACADA010000000
VERSION     DACADA010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.432"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(321..>587)
                     /locus_tag="ISB17_004982"
     CDS             complement(321..>587)
                     /locus_tag="ISB17_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1982795.1"
                     /translation="NRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPA
                     WPAVWLERDDDTGFQQVLERADYLAKEHTKAHCTGERNFGCSRS"
BASE COUNT          135 a          172 c          161 g          119 t
ORIGIN      
        1 cagtacgggc gcccgtcaga cgtccgttgc catcaagagc gataaaggat tttgcgtaca
       61 tagtcatatt cctcagccgc cagacgacac gcaggcaaca gccgtcccgg tacgggcagc
      121 gtggtcaggg tgtgaatggc ggttcagtta aaatctggac tggagtttgg gtaaagcagg
      181 ctggttgctg ttacagcact gtggttgtca cacaatggaa cactgacgta aaaaacagtc
      241 agtgggcgag tggatacatc gttgccgcca gacaacaacc catacttttg gtttgccggg
      301 tattggcgcg ccagtcgtgc tcaactccgg ctacagccaa aattgcgctc accggtgcag
      361 tgagctttgg tatgttcctt cgccagatag tcagcacgtt ccagcacctg ctgaaagcca
      421 gtgtcatcat cgcgttccag ccacaccgcc ggccaggccg gaaaatgcac cagcgtggca
      481 tggcacccga catccactcc cagggcactg caccacgcct gtttaatcat cccggccagt
      541 gatcccggag cacggtaatc accggcacga caccaggtat cccggtt
//
LOCUS       DACADA010000424          570 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.434, whole genome shotgun sequence.
ACCESSION   DACADA010000424 DACADA010000000
VERSION     DACADA010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.434"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(184..402)
                     /locus_tag="ISB17_004983"
     CDS             complement(184..402)
                     /locus_tag="ISB17_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAP1982796.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
BASE COUNT          161 a          129 c           94 g          186 t
ORIGIN      
        1 agtttattat cctggatgat atcaggagcg atatctataa agtttatgca ttccctgtga
       61 tcaatttttc ctgttagtga gcacaaattc aatctgattg atgccagaat aaaggcatct
      121 ggcatgttga ttttatgttc cagaatgaca aaacattgct gttaataagc acgactcttt
      181 ttgtcattcc tcatgccact taccctgttc ttcaacacca actacctcac ccctttgcct
      241 gtcacttttt gccttacgca ccggaaattg ctgacgctcc agatattgct ctaccgcttc
      301 gcgtaaaatt gcagagcgcg aaaccttacg ccagccccga agcttgttca gccgtttaat
      361 tgcctcttct gataaatgaa gaaaaaccgt attcatcgcc attgccacca ccttacatat
      421 attactcatt aatgtatgtg cgaaaattac gccgaaaaat catttaaaaa aagcggattt
      481 ttgcatgtcg ttttgttttt gcgagactgc atccaaagat aatgtaacag ttgcagttat
      541 ttcaattgtt actcaaaaat ggtttattac
//
LOCUS       DACADA010000425          568 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.435, whole genome shotgun sequence.
ACCESSION   DACADA010000425 DACADA010000000
VERSION     DACADA010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.435"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..342
                     /locus_tag="ISB17_004984"
     CDS             <1..342
                     /locus_tag="ISB17_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1982797.1"
                     /translation="TLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAG
                     MIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKAHC
                     TGERNFGCSRG"
BASE COUNT          116 a          152 c          170 g          130 t
ORIGIN      
        1 actctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcat
       61 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gtgattaccg cgctccggga
      121 tcactggccg gaatgattga acaggcctgg tgcagtgccc tgggggtgga tgccggatgc
      181 catgccacgc tggtgcattt tccggcctgg ccggcggtgt ggctggcgcg taatgatgac
      241 accggctttc agcaagtgct ggaacgtgct gactatctgg cgaaggagca taccaaagct
      301 cactgcaccg gtgagcgcaa ctttggctgc agtcgtggct gagccagaca gacgagttat
      361 ctgacttcag tgtgatgacg tgttcatggc caccacactg attttccctt atgagactga
      421 actgaaaccg ccattcacac ctgaccacgc tgcccgtacc gggacggctg ttgcctgcgt
      481 gtcgtctgac ggtaaggaac atatactatg tacgcaaaat cctttctcgc tcttgatagc
      541 aacggacgtc tgacgggcgc ccgtactg
//
LOCUS       DACADA010000426          565 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.436, whole genome shotgun sequence.
ACCESSION   DACADA010000426 DACADA010000000
VERSION     DACADA010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.436"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>565
                     /locus_tag="ISB17_004985"
     CDS             <1..>565
                     /locus_tag="ISB17_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP1982798.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYA"
BASE COUNT          100 a          190 c          175 g          100 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccga
//
LOCUS       DACADA010000427          564 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.437, whole genome shotgun sequence.
ACCESSION   DACADA010000427 DACADA010000000
VERSION     DACADA010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.437"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          145 a          159 c          126 g          134 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcctc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacatactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatcgctcc
      301 ctctccacgc cttatccgta acctgttttt tacattaaaa aaacaacgcc gggaattcaa
      361 tcgtcagtcc ggcgatgacc gttcgggtta tcacagagtg cctgagacag tgtcctgccg
      421 gaggtcacct caggggctct gcgcattttt tacgacgtgg ttattccgta tgaaccatac
      481 ggagatttaa ccatgaccta caaatacaac cccttctggc agcaacgtat tcgtgagacg
      541 gtgcggcacg cactgaatgt tcat
//
LOCUS       DACADA010000428          561 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.438, whole genome shotgun sequence.
ACCESSION   DACADA010000428 DACADA010000000
VERSION     DACADA010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.438"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>561
                     /locus_tag="ISB17_004986"
     rRNA            <1..>561
                     /locus_tag="ISB17_004986"
                     /product="16S ribosomal RNA"
BASE COUNT          142 a          125 c          185 g          109 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga g
//
LOCUS       DACADA010000429          542 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.439, whole genome shotgun sequence.
ACCESSION   DACADA010000429 DACADA010000000
VERSION     DACADA010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.439"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..366
                     /locus_tag="ISB17_004987"
                     /pseudo
     CDS             1..366
                     /locus_tag="ISB17_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          187 a          109 c          105 g          141 t
ORIGIN      
        1 agccaaataa ccatcaatga tctagcgaat gtagaaatat ctgctccttc gctaaatgtt
       61 caagatttaa tagtcaagag tctcaaagct tttgatgaca aaatcacaac actttcatcc
      121 atgaaccaaa ccttggaaca aatgtcccaa accctgttca aatcctggtt tgtggatttt
      181 gatccagtga ttgataacgc tctggatgca ggaaatccaa tcccggaagc cctgcaaact
      241 cgcgccaaat tgcgtcaaaa agtacgtaat agtgcagatt ttaaaccgct tccggcggaa
      301 attcgctcgc ttttcccaag tgaatttgaa gaaacggagt tgggttgggt gccaaagggg
      361 tggaaagaag ggacgcttcc agagatagct tttataaact ccacatcgtg gactaataaa
      421 aatcaaccag attatatcaa ttatgtagat ctctctaatg cgaaagatgg cagaatatac
      481 tcaattgaaa aattatcatt taatgatgct ccaagtcgag ctcgaagaat attaaaaaaa
      541 ga
//
LOCUS       DACADA010000430          540 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.440, whole genome shotgun sequence.
ACCESSION   DACADA010000430 DACADA010000000
VERSION     DACADA010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.440"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          145 a          152 c          119 g          124 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcccc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacaaactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatagctcc
      301 ctcgccacgc cttatccgta accggttttt tacattaaaa aaaacaacgc cgggaattca
      361 atcgtcagtc cggagacgac cgttcgggtt atcacagagt gcctgagaca gtgtcctgcc
      421 ggaggtcact tcagggactc tgcgtatttt ttacgacgtg gctattccgt atgaaccata
      481 cggagattta accatgacct acaaatacaa ccccttctgg cagcaacgta ttcgtgagac
//
LOCUS       DACADA010000431          539 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.441, whole genome shotgun sequence.
ACCESSION   DACADA010000431 DACADA010000000
VERSION     DACADA010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.441"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>539)
                     /locus_tag="ISB17_004988"
     CDS             complement(<1..>539)
                     /locus_tag="ISB17_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1982799.1"
                     /translation="STYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFPR
                     KTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLSD
                     SHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGALM
                     LNRLPPHDIYSGTVNALIK"
BASE COUNT          155 a          112 c          108 g          164 t
ORIGIN      
        1 attttaatta aagcgttaac ggttcctgaa tagatatcat gtggtggaag tcgatttaac
       61 atcagtgccc cctttagtgt agaacagact ataacggcga gctcatccgg aagaagaact
      121 gttttgaact ctccactatt ctgcccggag gtgataatat ctttaatcat ctgtatctcc
      181 tgatgtgcca gttgacttac agcctcctgt aattcaggag aaaactgatg gctgtcggat
      241 agcatggcat aaataccgca catttcccct ctttcagcac aaccagcaaa tgcatcaaga
      301 tatgtcctga gacgttgtac acctgaagaa acattatgga gagttatatt aagtctatca
      361 aatacttctg ttttgtattg gcagtaggct ataccaagat cagttttcct cgggaaatgg
      421 tgatgaatac tggcttttct gataccaatt tctttttcca gatctgcgta actgaacccc
      481 aaaaatccgc gttgctggat cagagtatcc gttatgcgaa gtatattctc ataagtaga
//
LOCUS       DACADA010000432          531 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.442, whole genome shotgun sequence.
ACCESSION   DACADA010000432 DACADA010000000
VERSION     DACADA010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.442"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            68..>531
                     /locus_tag="ISB17_004989"
     CDS             68..>531
                     /locus_tag="ISB17_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1982800.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVT
                     LPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYV
                     YPALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISNN"
BASE COUNT          168 a          113 c          100 g          150 t
ORIGIN      
        1 ctcacatctc tttgcccatc actatacaca taggaaaaat ttaagaaaat caagattgag
       61 gttgaaaatg ttttctaaga ataaaatcac catcgcagct atttcggctc tgcttatttc
      121 tggtaccgca atggcccaga caagcagtgg ttctgtctcc gctactggtt cttttaatgc
      181 cgcgacttgc agtgttaccc tgcctaatgc aacagcatct tttggcacag taacgaatca
      241 agaggtaaat gctgcaaaag cgtaccagaa aataaaaagt gtaaccgcag atcaggggat
      301 tagtgttacc gattgtacca ctcctgcatc actaacggta gcaactacta atttccagtc
      361 taatggctat gtttatcctg ctctaaacga ggctaccggt aatcaacaaa aggattttgc
      421 gctttggatg aaagtcggtg ataaaggtgt atcttcttat aatgcaccaa tcgaacttgc
      481 taatggtaca tatccaatcg agttgcaaat gattaaaata tcaaataact a
//
LOCUS       DACADA010000433          529 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.443, whole genome shotgun sequence.
ACCESSION   DACADA010000433 DACADA010000000
VERSION     DACADA010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.443"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(17..207)
                     /locus_tag="ISB17_004990"
                     /pseudo
     CDS             complement(17..207)
                     /locus_tag="ISB17_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          132 a          130 c          104 g          163 t
ORIGIN      
        1 aatgttggtg tatttattac accagcatta attttcctgg ggatgtatct tcctgttcat
       61 ttaaggtcaa ctgcctgacg tttctctccg aatattccat tatattaatg ttgacttgtt
      121 gaccagcttc gccctgtatg ctggcatcaa ctctctttta gactgaacac gccactcagt
      181 ttcctccctt tgcgacgcag cctgcattta cactcaaact gttaagatga taaatgtggt
      241 aaatctgttg gtactgacat aaaaacgttt acgccacagg aacggccaga tccatcggta
      301 accccatcgc cgacgttcga gtgccagtta gagtaacgcg cacagatgac tgaatgcagt
      361 gccctggcaa agaggccatc gttcctgtga caactggcag tcttcgttta acttcactta
      421 atttagctct tagggggctt accggacaga tgacgtactt acacctgttt aattttccat
      481 cacttattgg aatgaacacc cataaccatt ttgtgcggca tgttaatcc
//
LOCUS       DACADA010000434          527 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.444, whole genome shotgun sequence.
ACCESSION   DACADA010000434 DACADA010000000
VERSION     DACADA010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.444"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..448)
                     /locus_tag="ISB17_004991"
     CDS             complement(<1..448)
                     /locus_tag="ISB17_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HAP1982801.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLF"
BASE COUNT          155 a          104 c          102 g          166 t
ORIGIN      
        1 cgaataagta acgaacgcct ttcttgcctg atttataggt gagtattttg tatccatcgt
       61 aatcatattt acaggatgtt gtttcattat ttctatggaa ttcgacaatg ccgtcctcaa
      121 tgccaatcat ttcgatatct gtcgcataac ctttgcgata ataatcttta atttcagcaa
      181 atgttttggt tttatctgca tccgctttct tctgaaagac ggggtcaagt ttgccacttt
      241 gcagtaaagg gtaaacggat tgccagactc catcccagtc actgagcgtt cggttttgta
      301 cattggtatc atcaaaaaca ccattagcag ctttttgttc gacctctgtt aagggtttgc
      361 cgtgtgagtg atgaccatgc gaaaaggcag gagcgctaac aataaagaca cctaaagcaa
      421 cagccagttt atgaagacga atcgccaaaa caatttcctc cagagttaat gtacatgatg
      481 tgtaatgtta tattgtaaca tatgaaaaaa tgcaacaact cattagg
//
LOCUS       DACADA010000435          518 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.445, whole genome shotgun sequence.
ACCESSION   DACADA010000435 DACADA010000000
VERSION     DACADA010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.445"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>518
                     /locus_tag="ISB17_004992"
     CDS             <1..>518
                     /locus_tag="ISB17_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HAP1982802.1"
                     /translation="IGLFDCSRHSPTSSNNGLDYFFMWLGLPGKMIGSVLGEKAIDQD
                     SPLPDVVNNALHLVAAHERRPWRGLYLLGKKTDTANKKYREELLPGCSEDIGGGLKPD
                     EQKPSAELCRVALYNMYHAACQAGVPFPDFDTLDQYDTKVASYFIMNDAVQGGSVNYW
                     VSRYQREEVKDK"
BASE COUNT          149 a           83 c          135 g          151 t
ORIGIN      
        1 tattggtctt tttgactgtt ctcggcattc accgactagt agtaataatg ggttggacta
       61 tttttttatg tggttgggtt taccagggaa aatgattggt tcagtattag gtgaaaaagc
      121 tattgatcag gactcaccac tgccggacgt agtaaataac gcacttcacc ttgtagcagc
      181 tcatgaacgg cgtccatggc gtggtctgta tctgttgggg aaaaaaacgg atacagcgaa
      241 taaaaaatac agggaagaac tgcttcccgg ttgtagtgaa gatattggtg gtggcttgaa
      301 accggatgaa cagaaaccca gtgcagaact gtgcagggtt gcactataca atatgtatca
      361 tgcagcatgt caagctggtg tgccatttcc tgattttgac actttggatc aatatgatac
      421 gaaagttgcc agctatttta ttatgaatga tgctgttcag ggaggatctg taaattactg
      481 ggttagtcgt tatcaaagag aggaagtgaa ggataaag
//
LOCUS       DACADA010000436          512 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.446, whole genome shotgun sequence.
ACCESSION   DACADA010000436 DACADA010000000
VERSION     DACADA010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.446"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..215)
                     /locus_tag="ISB17_004993"
     CDS             complement(<1..215)
                     /locus_tag="ISB17_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAP1982803.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRT"
     gene            complement(258..494)
                     /locus_tag="ISB17_004994"
     CDS             complement(258..494)
                     /locus_tag="ISB17_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAP1982804.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          139 a          115 c           90 g          168 t
ORIGIN      
        1 gttcttcggt ttggccctcc tgtatcactg ttgctaaaat cacgaattat gctgtcaatt
       61 aaatctcgtc ggaaagtatt ttcctcagcc gatttcatta cgagtaattg cttaataaga
      121 gtctgcaacg aaaaatttat cttcatgcgc agtatctgga gcaccgcctg gccagtaatt
      181 tcatatttaa tactttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa
      241 ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt
      301 tcctcttccc ctgtcgcttg ttccagtcgg caaagaagtt tggcacaaag cgactttcga
      361 ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga
      421 gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ctgcagaatc tgaagtcact
      481 gaattttgat gcatgctacg cctctgttaa aa
//
LOCUS       DACADA010000437          506 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.447, whole genome shotgun sequence.
ACCESSION   DACADA010000437 DACADA010000000
VERSION     DACADA010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.447"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>506
                     /locus_tag="ISB17_004995"
     CDS             <1..>506
                     /locus_tag="ISB17_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1982805.1"
                     /translation="TVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHIL
                     SGVTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTER
                     SASTIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFR
                     SDSGLIGAT"
BASE COUNT          139 a           68 c          136 g          163 t
ORIGIN      
        1 acagttttgc cgaaagaaat ttgtgatatg gtttttataa ccgtttctac gggggttgga
       61 gggggaattg tagttaacag aaaattatta acaggtgttt ctggattagc cggacatgtt
      121 gggcatatat tgtccggagt aacggataca gaatgtggat gtggtcgtcg gggatgcgtt
      181 gaagcggttt catctggtcg ggccatcatg ggggcagcaa aaaataaact tgcaggatat
      241 agcaccaaat atatttttga attagcgaga cagggatata aggaagcaga atttttgaca
      301 gagcgttctg ccagtactat tgctgaatta atagtaagtt taaaattact gcttgattgt
      361 caggttgttg ttgtcggagg aagtgttgga cttgctgatg ggtatgttca gaaagtgtcg
      421 aaacatcttt ccatatattc tgaaatttgt aatgtaatgc tgtttcctgc atatttcaga
      481 agtgactctg gtcttattgg tgcaac
//
LOCUS       DACADA010000438          500 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.449, whole genome shotgun sequence.
ACCESSION   DACADA010000438 DACADA010000000
VERSION     DACADA010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.449"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          153 a          117 c           87 g          143 t
ORIGIN      
        1 tcaacataaa aaattcagca aaatttaata ttcgttcaac tagccgtctc acatatgaca
       61 ttattagtca actccattta ccacaaaatg gctatttcat ggaacgcagt aatttcttag
      121 tggctattat caagctaatt cttaggtccc tagcgattat tcctggctgg ttcgtcagtt
      181 taggtcatga gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg
      241 ctttttcatc tacatccgtt ggtaaatatg gctttggtca ctacgactta aaccctcctc
      301 aatcacagct actcgccggg cgatatggca aacaaactaa ttgaaacatc atcctgaaaa
      361 caccagcatc aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa
      421 ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta aacacttatt
      481 ttcaccaagc tcactgagtt
//
LOCUS       DACADA010000439          498 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.450, whole genome shotgun sequence.
ACCESSION   DACADA010000439 DACADA010000000
VERSION     DACADA010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.450"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(49..234)
                     /locus_tag="ISB17_004996"
     CDS             complement(49..234)
                     /locus_tag="ISB17_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982806.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            283..441
                     /locus_tag="ISB17_004997"
                     /pseudo
     CDS             283..441
                     /locus_tag="ISB17_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          142 a          127 c           96 g          133 t
ORIGIN      
        1 aaatgtaatg tttctctcag agaatataaa tcattccttt tgcccacctt aatggtgggc
       61 ttttttatgc aaaacctgaa cgtcctgtgg tattcctctg gcataatgat tttcgcgatt
      121 aaccaacaac cagaccagca aagacgccac cacaagcaca gtaacatcca tcaggcttgc
      181 cacaccccat gcgtgcggct caaagccgcc atggcatatt aatccgcttg ccattttcga
      241 acagctgatt ggtttggtcc ggtgtttccc tgcttgcact taaaaatgag cggataggaa
      301 actctcgaaa tggttcgtcg atatgctcac ctcgcaccta atcaccttac cgaacacgca
      361 cggtaaatag attcgatcct gaacccatcg gtcccaaatc tgtcccaatc aaaaaataag
      421 gaaggtatta atgatgtgta acttattgat ttaagtggtg ccgataatag gagtcgaacc
      481 tacgaccttc gcattacg
//
LOCUS       DACADA010000440          484 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.451, whole genome shotgun sequence.
ACCESSION   DACADA010000440 DACADA010000000
VERSION     DACADA010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.451"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>484
                     /locus_tag="ISB17_004998"
     rRNA            <1..>484
                     /locus_tag="ISB17_004998"
                     /product="16S ribosomal RNA"
BASE COUNT          119 a          119 c          143 g          103 t
ORIGIN      
        1 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
       61 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag
      121 gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac
      181 aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg
      241 tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga
      301 tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg
      361 agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat
      421 tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcacct
      481 cctt
//
LOCUS       DACADA010000441          479 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.452, whole genome shotgun sequence.
ACCESSION   DACADA010000441 DACADA010000000
VERSION     DACADA010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.452"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>479)
                     /locus_tag="ISB17_004999"
     CDS             complement(<1..>479)
                     /locus_tag="ISB17_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     endonuclease"
                     /protein_id="HAP1982807.1"
                     /translation="DRFGERIYLDLPHLFSTKSITIPDISSNLEAWIEYLKEQYIPQE
                     SRMLSPGAMANPPDRFDGFHSFNRCCRSIADKGRTKENLKSYVTDRRVFEYWVDGDWV
                     AADRLMGQVRTNNIFINEECLNAGNGGLHPTPCQADHIGPISLGFSHRPQFQLLCKS"
BASE COUNT          143 a           91 c           92 g          153 t
ORIGIN      
        1 tgatttgcaa agtagttgaa attgtgggcg atgggaaaac cctagtgaga ttgggccaat
       61 gtgatctgct tggcaaggag ttggatgtaa accaccattt ccggcattca aacattcctc
      121 gttaataaat atattatttg ttctaacttg gcccattagc ctgtctgcag ctacccaatc
      181 accatccacc caatattcga aaactcttct gtcagtaaca taagatttaa gattttcttt
      241 ggtccttcct ttatcagcaa tagagcgaca acagcggtta aatgaatgaa atccatcaaa
      301 acgatctgga ggatttgcca tggctccagg gctaagcata cgtgattctt gaggtatgta
      361 ttgttctttt agatactcaa tccatgcttc gagattgctt gaaatatctg gaatcgtgat
      421 ggattttgta gaaaataaat gtggtaaatc taaatatatt ctttcaccaa atctatcat
//
LOCUS       DACADA010000442          475 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.453, whole genome shotgun sequence.
ACCESSION   DACADA010000442 DACADA010000000
VERSION     DACADA010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.453"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          144 a          103 c           96 g          132 t
ORIGIN      
        1 aaacttaggc gaaaggaagt actgaagtga cagaataaat cacacttttt gtacatatgt
       61 acttttctat tgtcttttgt tgtggaaaca aatagtgttt gtcatgccga tgctaataga
      121 cagatagatt cgagggccaa cagccccatc atcatgatgg agcaacagag gtgagtctaa
      181 cgtttccgca cttcttatcg atcggcacta acgatcaata tcgataaact gatctatcaa
      241 aacgattatc tttcttgcaa cctgcctggt tatccttctc ttacattgct cgcagaaaag
      301 cccttttatt acggttagtt atctagctga gacttgctac gcccattttc catgtacccc
      361 aaatcacgag gtcacaaagg cgcggaaaga tgaagagatg tgcacgttgc aacaaaaaaa
      421 gaagcgtcct tgaacagagg ctctcactcg ataaagaatt ctgcgacgag tgctt
//
LOCUS       DACADA010000443          465 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.454, whole genome shotgun sequence.
ACCESSION   DACADA010000443 DACADA010000000
VERSION     DACADA010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.454"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>465
                     /locus_tag="ISB17_005000"
     CDS             <1..>465
                     /locus_tag="ISB17_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP1982808.1"
                     /translation="RQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQKRFLTKQ
                     KRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDLQGVGG
                     EPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVV"
BASE COUNT           87 a          150 c          152 g           76 t
ORIGIN      
        1 cggcaaggca gcggcctcgt gacggatctg gtgcccggcc agtccggtgg aggtaaaggt
       61 aaggggcgct tgccggaacc ggtcgagcgc gtcatccacg agctactgca aaagcggttc
      121 ctgaccaagc agaagcgcag cctagcggcc tttcaccgcg aagtcactca ggtgtgcaag
      181 gctcaaaaac tgcgagtgcc ggcgcgcaat accgtggcct tacggatcgc tagccttgac
      241 ccgcgcaagg tcatccgccg gcgggaaggc caggatgccg ctcgtgacct acaaggtgtg
      301 ggcggcgagc ctcctgccgt gaccgcgccg ctggagcagg tgcagataga ccatacggtc
      361 atcgacctga tcgtggtcga tgaccgcgac cggcaaccta ttggccgccc gtacctgacc
      421 ctcgccatcg acgtgttcac ccgctgcgtg ctcggcatgg tcgtc
//
LOCUS       DACADA010000444          460 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.455, whole genome shotgun sequence.
ACCESSION   DACADA010000444 DACADA010000000
VERSION     DACADA010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.455"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            172..444
                     /locus_tag="ISB17_005001"
                     /pseudo
     CDS             172..444
                     /locus_tag="ISB17_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          108 a          114 c          114 g          124 t
ORIGIN      
        1 ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg tcagaatgac
       61 tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatggg gatttcggta
      121 atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc
      181 atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc
      241 actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc
      301 acttacaccg cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc
      361 gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt tttacgtcac
      421 ttaaaaaact caggccgtag tcggtaacct cgcgcataca
//
LOCUS       DACADA010000445          460 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.456, whole genome shotgun sequence.
ACCESSION   DACADA010000445 DACADA010000000
VERSION     DACADA010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.456"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..374)
                     /locus_tag="ISB17_005002"
     CDS             complement(<1..374)
                     /locus_tag="ISB17_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1982809.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT          127 a           96 c          101 g          136 t
ORIGIN      
        1 ccaatggtgt tggtgccaac ggcctgacca tttttcggtg caggcgcatg ccacagatcg
       61 gcaaccatgt ttacgcaacg cagatcgatt gctgcagatt gcggatattc ttctttgtcg
      121 atccagtttt tgttaatgga taaatccatc aacttatgga cattttcgtc gtcccaggtc
      181 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc gtcattgata
      241 atctggaatg cgacatcatc acgcatttcg tgcagcggaa aacgttttga ttctgcgata
      301 gtggaaatag acttcgcacc aaaacgtgaa tcgagtagtt ccgaccttaa atccgttact
      361 tgcttcttat ccattttaaa ctccttaaaa tgattggatc gcattaaaaa agtaggattt
      421 atcgataaag taagcaagtg caaaggactc ggttttaaat
//
LOCUS       DACADA010000446          460 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.457, whole genome shotgun sequence.
ACCESSION   DACADA010000446 DACADA010000000
VERSION     DACADA010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.457"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..402
                     /locus_tag="ISB17_005003"
     CDS             <1..402
                     /locus_tag="ISB17_005003"
                     /inference="COORDINATES: protein motif:HMM:NF022343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAP1982810.1"
                     /translation="YRNLKITSALDEIYGGICKKAHAATELAKGLRLNLFMKGPFDPV
                     EDYTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGV
                     ANSSGFALAALLNANIELGNDPIIGIEAYPG"
     gene            425..>460
                     /locus_tag="ISB17_005004"
     CDS             425..>460
                     /locus_tag="ISB17_005004"
                     /inference="COORDINATES: protein motif:HMM:NF022343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982811.1"
                     /translation="MTLQPSSLPELF"
BASE COUNT          142 a           95 c           96 g          127 t
ORIGIN      
        1 tatcgaaact tgaaaatcac ctcagcttta gacgaaatat atggtggtat atgtaaaaag
       61 gcgcatgctg ccacagagtt agcaaaagga ttacggctaa atttatttat gaaaggccct
      121 ttcgatcctg ttgaggacta tacagttcat gaaataacat taggccctgg ttgcaatgtt
      181 cctggttatg ccggaaccac gataggatat atttcaaccc ttccagcttc tcaggcaaaa
      241 agatggacca atgagcagcc aaggattgat atctatattg atcagataat gacggtaaca
      301 ggggtagcaa attcttccgg attcgccctt gccgcattac ttaatgctaa tattgagttg
      361 ggtaatgacc caataattgg tatagaagcc tatcccgggt gatgaaaatg caccactcaa
      421 acgtatgact ctgcaaccgt catctcttcc agaactgttt
//
LOCUS       DACADA010000447          455 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.458, whole genome shotgun sequence.
ACCESSION   DACADA010000447 DACADA010000000
VERSION     DACADA010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.458"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..374)
                     /locus_tag="ISB17_005005"
     CDS             complement(<1..374)
                     /locus_tag="ISB17_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1982812.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT          122 a           99 c          104 g          130 t
ORIGIN      
        1 ccaatggtgt tggtgccaac ggcctgacca tttttcggtg caggcgcatg ccacagatcg
       61 gcaaccatgt ttacgcaacg caggtcgatt gctgcagatt gcggatattc ttctttgtcg
      121 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
      181 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
      241 atctgaaatg caacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
      301 gtagaaatgg cctttgcacc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
      361 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
      421 atccgcaatg gaagcaaggc attacaggct taatt
//
LOCUS       DACADA010000448          455 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.459, whole genome shotgun sequence.
ACCESSION   DACADA010000448 DACADA010000000
VERSION     DACADA010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.459"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            14..>455
                     /locus_tag="ISB17_005006"
     CDS             14..>455
                     /locus_tag="ISB17_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1982813.1"
                     /translation="MNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSTD
                     KAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARYEVRLE
                     PRMALLTRSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT          106 a          147 c          116 g           86 t
ORIGIN      
        1 ggatgttcct gttctgaatt tcgaccactc acaccacaag ctcaaaattc gcggcctgca
       61 aagtccggtg gatgtgctga ccttcgaagg ccgcgaacaa ctgagtaccc cgttccgcta
      121 cgacattcag tttaccagta ccgataaagc catcgcgccg gagtcggtgc tgatgcagga
      181 cggcgcattc agcctgacgg caccaccggt acagggaatg ccggtgcaga ctgccctgcg
      241 caccctgcac ggcgtcatta ccagctttaa acacctctcc tcgtcacagg acgaagcccg
      301 ttacgaagtg cgccttgaac cccgtatggc gctgcttacc cgcagccgcc agaacgccat
      361 ctaccagaat ctgacggtgc cgcaaatcgt cgaaaaaatc ctgcgcgagc ggcaccagat
      421 gcgcggtcag gacttcgtgt ttaacctcaa aagcg
//
LOCUS       DACADA010000449          442 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.460, whole genome shotgun sequence.
ACCESSION   DACADA010000449 DACADA010000000
VERSION     DACADA010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.460"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..259
                     /locus_tag="ISB17_005007"
                     /pseudo
     CDS             1..259
                     /locus_tag="ISB17_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            345..>442
                     /locus_tag="ISB17_005008"
     CDS             345..>442
                     /locus_tag="ISB17_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP1982814.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
BASE COUNT          103 a          114 c          132 g           93 t
ORIGIN      
        1 gctgtctccg ctggaagagg cgcttcatgg ctatgtgctg actgacggta agctccatgc
       61 tgatgacacg cctgtcccgg tgctgttgcc aggcaataag aaaacgaaga ccgggcggtt
      121 atggacctac gttcgtgacg accgtaacgc cgggtcaacg ctggcgccgg cggtgtggtt
      181 cgcttacagc ccggacagaa aaggcatcca tccgcagacc catcttgcgg ggttcagtgg
      241 tgtactgcag gcggatgctg tcacgaacgg tgcaatagtg ctccacaccc aacgcctgaa
      301 atcagatcca gggggtaatc tgctctcctg attcaggaga gtttatggtc acttttgaga
      361 cagttatgga aattaaaatc ctgcacaagc agggaatgag tagccgggcg attgccagag
      421 aactggggat ctcccgcaat ac
//
LOCUS       DACADA010000450          441 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.461, whole genome shotgun sequence.
ACCESSION   DACADA010000450 DACADA010000000
VERSION     DACADA010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.461"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>441
                     /locus_tag="ISB17_005009"
     CDS             <1..>441
                     /locus_tag="ISB17_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1982815.1"
                     /translation="LPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGEYLPSERE
                     LMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIVKDFLSTA
                     DGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLR"
BASE COUNT          144 a           91 c           97 g          109 t
ORIGIN      
        1 ttacccgggc taacaacgac cagaaagccc attgttacaa aaaaactttc agaaatcgtt
       61 gccgaagagc ttgaaagaat gatccgccag gaggagataa gggaaggtga atacctgcct
      121 tctgaacgtg aacttatggc attctttggc gttggaagac catctgtcag agaggcttta
      181 tcatctctca gacataaagg gctaatccgg tataacaagg gagaaaaagc cagtgtctgc
      241 cgtccatcac cagaaaccat cattaatggg ttatcaggca ttgttaaaga ttttttatct
      301 acagctgatg gcatcaagga ttttgaaaga ctacgcatct tttttgaaac caaccttgta
      361 agatatgcag caaaaaatgc aacgcctgag gatattcaca ggcttgagaa ggtgctcagt
      421 atccatcata actcactgag a
//
LOCUS       DACADA010000451          426 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.462, whole genome shotgun sequence.
ACCESSION   DACADA010000451 DACADA010000000
VERSION     DACADA010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.462"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          127 a           97 c           86 g          116 t
ORIGIN      
        1 agtcagggga tattccatag aactgaacgt tcatccccca gaatgagaag ttgaacatgc
       61 ctccatagcg aatagtgttc cggactcgaa tcgttgaacg atatcctgca atttggagca
      121 tgaggtgttt ctatcctatg caacagaatc atcattaatc cagatatagg cctgaaaaac
      181 aggcctggca atatcgatac caacaatcct gatcgtgttc atgggatgtt actctcggat
      241 taatgcagtc agtaaaagta tggcactgac tgcattaaga agggacgtcc atcatatcgt
      301 gacacaaacg ctcccaccga catcgccatt gagtcctgca ccgtatcggt gaacttgtaa
      361 ctatcccgta taatcattct atacacattt attggcttga tgagtcaaat acttcttaat
      421 ttcgga
//
LOCUS       DACADA010000452          416 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.463, whole genome shotgun sequence.
ACCESSION   DACADA010000452 DACADA010000000
VERSION     DACADA010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.463"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            43..>416
                     /locus_tag="ISB17_005010"
     CDS             43..>416
                     /locus_tag="ISB17_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAP1982816.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DACADA010000453          405 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.465, whole genome shotgun sequence.
ACCESSION   DACADA010000453 DACADA010000000
VERSION     DACADA010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.465"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>405
                     /locus_tag="ISB17_005011"
     CDS             <1..>405
                     /locus_tag="ISB17_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982817.1"
                     /translation="QSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGI
                     YLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQ
                     TLELVFTAGSATVTPKLNGVAGPAFQVIKDSLT"
BASE COUNT          101 a          105 c          116 g           83 t
ORIGIN      
        1 cagagcaata aagcctggtc cctgacgcgc ccggtggatg acgcagtctc cctgctgaca
       61 cggggtggca gactgagctg taagtttcga ctgtcaggcg cactgaccaa caatcagttc
      121 ggtctgggaa tttatctgta taccgatgta gcgttacctg acgtcgtggc gatgaccggg
      181 actggtaacc cgttcctgat gtcgttcttc acccagacca cagacggcaa actgaatctg
      241 atgcatcaca agaaagcagg aaacacaaag ttgggcgagt tcgggaatta cagtaacgac
      301 tggcagacgc tggagctggt gttcaccgcc ggcagtgcca cggttactcc gaaactgaat
      361 ggagtggctg gcccggcatt ccaggtcata aaagacagtc tgaca
//
LOCUS       DACADA010000454          397 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.466, whole genome shotgun sequence.
ACCESSION   DACADA010000454 DACADA010000000
VERSION     DACADA010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.466"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            25..>397
                     /locus_tag="ISB17_005012"
     CDS             25..>397
                     /locus_tag="ISB17_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1982818.1"
                     /translation="MPLSGGQLNGALGIGTSSALGGNSIVLGDNDTGFKQNGDGNLDV
                     YANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVKDVRLGSQQYYGVNNWQT
                     WNFQCPSGHVLSGINVQDTGSN"
BASE COUNT          105 a           73 c           98 g          121 t
ORIGIN      
        1 ccttcagcag gagatgttgg tgcactgccg ttatctggtg gtcaactgaa tggtgcactg
       61 ggtatcggaa catccagtgc tcttggcggt aattcgattg tattgggtga taatgacacg
      121 ggctttaaac aaaatggcga tggtaatctg gatgtttatg ctaataacgt ccatgttatg
      181 cgctttgtct ccggaagcat tcaaagtaat aaaaccataa atattacagg gcgtgttaac
      241 ccctcggatt acggtaactt tgattcccgt tatgtgaaag atgttcgact tggttcacag
      301 caatattatg gagtgaacaa ctggcaaaca tggaatttcc agtgcccttc aggtcatgta
      361 ttgtctggta ttaatgttca ggatacaggg tctaact
//
LOCUS       DACADA010000455          389 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.467, whole genome shotgun sequence.
ACCESSION   DACADA010000455 DACADA010000000
VERSION     DACADA010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.467"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            79..270
                     /locus_tag="ISB17_005013"
     CDS             79..270
                     /locus_tag="ISB17_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAP1982819.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT           95 a          103 c           76 g          115 t
ORIGIN      
        1 aagaattgac cattttgtcc tgttgtgcct taatgtaagt accgtccacg gcgtgggaca
       61 tacttcaagg aaccttttgt gagtcaggca accagtatgc gaaaacgaca ccgatttaac
      121 agtcgcatga cccgtatcgt actgctcatc agctttatct tcttctttgg ccgttttatc
      181 tactcgtccg tcggtgcctg gcagcaccat cagagcaaaa aagaagctca gcaatccaca
      241 ctctccgtcg aatcaccggt acaacgttag cggttacctt ctccactctc acagaacata
      301 acggcacctc gctgtcggat gcttttgctc tttgagatta tcaaagcggc agatattctt
      361 tcatcttaaa ttttacgtct ttgttctga
//
LOCUS       DACADA010000456          389 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.468, whole genome shotgun sequence.
ACCESSION   DACADA010000456 DACADA010000000
VERSION     DACADA010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.468"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>389
                     /gene="rfaJ"
                     /locus_tag="ISB17_005014"
     CDS             <1..>389
                     /gene="rfaJ"
                     /locus_tag="ISB17_005014"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide glucosyltransferase RfaJ"
                     /protein_id="HAP1982820.1"
                     /translation="AEQYQLRITLYRINTDKLQCLPCTQVWSRAMYFRLFAFQLLGLT
                     LDRLLYLDADVVCKGDISQLLHLDLNGAVAAVVKDVDPMQEKAASRLSDPELLGQYFN
                     SGVVYLDLKKWANAKLTEKALSILMSK"
BASE COUNT          114 a           67 c           83 g          125 t
ORIGIN      
        1 tgcagagcaa taccaattaa gaatcacatt atatagaatt aacactgata agctccagtg
       61 cttgccttgt acacaggtct ggtcaagagc aatgtacttc cgtttatttg catttcaatt
      121 attgggttta acacttgatc gtttgcttta tcttgatgca gatgttgttt gtaaaggcga
      181 tattagccaa ctattgcatc tggatttaaa cggcgcggtt gctgctgttg ttaaagatgt
      241 tgacccaatg caggaaaagg cggcatccag gttgtctgat ccagaattac tcgggcagta
      301 ctttaattcc ggtgtcgttt atttagattt aaaaaaatgg gctaacgcaa aactaacaga
      361 aaaagcgttg tctattctta tgagtaaag
//
LOCUS       DACADA010000457          377 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.470, whole genome shotgun sequence.
ACCESSION   DACADA010000457 DACADA010000000
VERSION     DACADA010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.470"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>377)
                     /locus_tag="ISB17_005015"
     CDS             complement(<1..>377)
                     /locus_tag="ISB17_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982821.1"
                     /translation="NRQTFSGGLSGELSGNASTAAKLKTARKISNVAFDGSSDITLKA
                     SHVGAFALGKTGSTVANDKAVGWNWSSGAYNATISGASTLIIHFYMGEGSCPAAQFRI
                     NYKNGGIFYRSARDGYGFEADWS"
BASE COUNT          109 a           93 c           67 g          108 t
ORIGIN      
        1 tcggaccagt cggcttcaaa accataacca tcacgggctg aacgataaaa aataccgcca
       61 tttttataat taatccgaaa ctgagctgca ggacaacttc cttctcccat ataaaaatga
      121 ataattaacg ttgatgcacc actaatagtt gcgttatagg ctccgctact ccagttccat
      181 ccaactgctt tatcattcgc aacggtgctt cctgttttcc ctaaggcaaa cgcaccaaca
      241 tgacttgctt ttaatgtgat atcggaggaa ccatcaaaag ccacattgct tattttcctg
      301 gcagttttta attttgcagc tgtagaagca ttgccggata gttcaccaga aaggccaccg
      361 ctgaatgttt gtcgatt
//
LOCUS       DACADA010000458          371 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.471, whole genome shotgun sequence.
ACCESSION   DACADA010000458 DACADA010000000
VERSION     DACADA010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.471"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>371
                     /locus_tag="ISB17_005016"
     CDS             <1..>371
                     /locus_tag="ISB17_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HAP1982822.1"
                     /translation="STKNWTHAIYFRFVIADYFISKVAKVLYLDADIICQGTIEPLIK
                     FSFPDDKVAMVVTEGQADWWEKRAHSLGVAGISKGYFNSGFLLINTAKWAAQQVSARA
                     IAMLNEPEVIKKITHPDQDVL"
BASE COUNT          110 a           66 c           80 g          115 t
ORIGIN      
        1 ctagcacaaa gaactggact catgcaatat attttcgttt tgttattgct gattacttta
       61 ttagtaaggt ggctaaagtc ctttatctgg atgcagatat catttgtcag gggactattg
      121 agccactaat taaattctca tttcctgacg ataaagtcgc aatggttgtt acagaagggc
      181 aagctgactg gtgggaaaaa cgcgcccatt cgttaggtgt tgcgggaatt tctaaaggtt
      241 actttaactc cggtttttta ttgattaata ctgccaaatg ggcagctcag caggtttctg
      301 cacgagccat tgcaatgcta aatgagccag aagtaatcaa aaaaataaca catcctgatc
      361 aggatgtatt a
//
LOCUS       DACADA010000459          369 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.472, whole genome shotgun sequence.
ACCESSION   DACADA010000459 DACADA010000000
VERSION     DACADA010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.472"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..369
                     /locus_tag="ISB17_005017"
                     /pseudo
     CDS             1..369
                     /locus_tag="ISB17_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a           94 c           89 g           74 t
ORIGIN      
        1 atgtcggcct ttccacaatg acgcgatggg tgaaacaatt acgtgatgag cggcagggaa
       61 aaacaccaaa agcctcccct attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      121 agctacaacg tattgaaatg gaaaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      181 cagactccct gaacagttct cgataatcgg gaaactcagg gcgcgttatc ctgtggccac
      241 tctctgccat gtgttcgggg tccatcgcag cagctacaaa tactggaaaa accgtcctga
      301 aaagccagac ggcagacggg ctgtattacg cagccaggta cttgaactgc atggcatcag
      361 ccacggctc
//
LOCUS       DACADA010000460          364 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.473, whole genome shotgun sequence.
ACCESSION   DACADA010000460 DACADA010000000
VERSION     DACADA010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.473"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..209)
                     /locus_tag="ISB17_005018"
     CDS             complement(<1..209)
                     /locus_tag="ISB17_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP1982823.1"
                     /translation="MENNTSLETTDKTNIVTYGENAVGVLACSSPGESRTCVDAVDDE
                     VCDSNSYEVISRADLKMNGGSITTNG"
BASE COUNT           99 a           97 c           55 g          113 t
ORIGIN      
        1 ccattagttg ttatggaacc accattcatt tttaaatcag cacggctaat aacttcgtaa
       61 ctgttagaat cacaaacttc atcatctaca gcatcgacac atgttcgaga ctctccagga
      121 cttgaacatg caagaactcc tactgcattt tccccatagg taacaatgtt cgttttatct
      181 gtggtttcaa gactggtgtt attttccaca gtcccagaat cattaacctg acttaaaaat
      241 cccgctgaat atgccccctc gtctcgactg aattatcacc attcataacg atggtgccac
      301 caaattcact gactactgcg ctggctgtta tccctccatt ttctttgtca gatacgcttc
      361 caga
//
LOCUS       DACADA010000461          360 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.474, whole genome shotgun sequence.
ACCESSION   DACADA010000461 DACADA010000000
VERSION     DACADA010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.474"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(144..>360)
                     /locus_tag="ISB17_005019"
     CDS             complement(144..>360)
                     /locus_tag="ISB17_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982824.1"
                     /translation="NNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTIN
                     LLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          118 a           77 c           70 g           95 t
ORIGIN      
        1 aaattatttc actgatatat aaaacaactg tgataacacc agggttgaca actatttgca
       61 caataattcc tttccttcaa tgatccaggg gttcactgcc agcaacccat tcagccgcat
      121 actgacagtg agaaactgaa agattagtgc tgatccctgt ggctgacgag tctttccctt
      181 aatcgattaa ccagattgat cgccagccaa acaagggcat taagcaaatt tatcgtcaca
      241 agcacttctc ccagcccggt gctgggtttt gcaataagga tgaccaggag agtgatcaac
      301 ataaatgcaa tggaaaagat gatatagaga gtgaaataat ccagattatt actattgttc
//
LOCUS       DACADA010000462          358 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.475, whole genome shotgun sequence.
ACCESSION   DACADA010000462 DACADA010000000
VERSION     DACADA010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.475"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..294
                     /locus_tag="ISB17_005020"
     CDS             <1..294
                     /locus_tag="ISB17_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1982825.1"
                     /translation="KLLTRLNNSDLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSII
                     VVSQLPVENWYKMIGDSTHADAILDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          109 a           78 c           90 g           81 t
ORIGIN      
        1 aaacttttga ccaggctgaa taatagcgat ctgctgcttc tggatgactg ggggctggaa
       61 cctctctcat cagaacagcg tagcgacctg ctggaaatag tggatctgat gtaccaacga
      121 ggctcaatca tcgtagtgag ccagttgccg gtggaaaact ggtacaaaat gatcggagac
      181 tccacacatg cggatgccat cctagatcga ctggttcatg gcagtatcaa gatcgaactt
      241 aaaggagaat caatgcggaa aatacaatct ccgttgaccg aaggagatca gtgaaggtaa
      301 tttaaaaacg gttctgtgaa agtgacacga accgatctcc atcgatgtta ctcaccga
//
LOCUS       DACADA010000463          352 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.476, whole genome shotgun sequence.
ACCESSION   DACADA010000463 DACADA010000000
VERSION     DACADA010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.476"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT          115 a           70 c           71 g           96 t
ORIGIN      
        1 acaacgttta tcgacctcac acgaacaacg atggtttacc gccttgctta catgaagaaa
       61 agtggaaaca ggttattccg gtgtcctgat tttgtgaacc actacaataa tcctttttca
      121 cagacttgtc agcagccagc atttatgttc ttttacctgc gggaatttat ccgtctgcct
      181 gtcgggacta ctctgtcata cagaaatcag gtaggaataa attgtagtgg aaagtcgatg
      241 tttaccggat gactgatgcg cgcttgtaca cagacagtgt gtttcagtaa tatggagaat
      301 aatgaaatga ataacacaga cacattagaa aaaataatca gacaccaaaa aa
//
LOCUS       DACADA010000464          344 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.477, whole genome shotgun sequence.
ACCESSION   DACADA010000464 DACADA010000000
VERSION     DACADA010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.477"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>344
                     /locus_tag="ISB17_005021"
     CDS             <1..>344
                     /locus_tag="ISB17_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1982826.1"
                     /translation="GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVE
                     LREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADT
                     GIDIDMHIDAASG"
BASE COUNT           80 a           97 c          103 g           64 t
ORIGIN      
        1 ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagccgcagg caaacccacg
       61 gataaaccaa acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac
      121 tgggatgtgg agttgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa
      181 cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc
      241 tacaccggta actatgagtt cccgcagccg ctgcacgatg cgctggataa attccaggcc
      301 gacaccggta tcgacatcga catgcacatc gacgccgcca gcgg
//
LOCUS       DACADA010000465          340 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.478, whole genome shotgun sequence.
ACCESSION   DACADA010000465 DACADA010000000
VERSION     DACADA010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.478"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>340)
                     /locus_tag="ISB17_005022"
     CDS             complement(<1..>340)
                     /locus_tag="ISB17_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAP1982827.1"
                     /translation="VIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYE
                     IASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTL
                     VDATIIEAPSS"
BASE COUNT           72 a           86 c          110 g           72 t
ORIGIN      
        1 gagctgggtg cctcaatgat ggtggcatcc accaaagtgc cttgggtcat catgacgcct
       61 gcttcggcca gccagcgatt gatggtcttg aacaattgac gggccagttg atgctgctcg
      121 agcaggtggc ggaaattcat gatggtggtg cgatccggca gggcgctatc cagggataat
      181 cgggcaaaca ggcgcatgga ggcgatttcg tacagggcat cttccatggc accgtcgctc
      241 aggttgtacc aatgctgcat gcagtgaata cgcagcatgg tctccagcgg atagggccgt
      301 cggccattgc ccgccttggg ataaaacggc tcgatgacag
//
LOCUS       DACADA010000466          328 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.480, whole genome shotgun sequence.
ACCESSION   DACADA010000466 DACADA010000000
VERSION     DACADA010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.480"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>328)
                     /locus_tag="ISB17_005023"
     CDS             complement(<1..>328)
                     /locus_tag="ISB17_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAP1982828.1"
                     /translation="YGITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCVLTLSLLVL
                     LIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKML
                     HANSSSS"
BASE COUNT          133 a           78 c           50 g           67 t
ORIGIN      
        1 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
       61 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
      121 aaaactaaat cttacgccgt tgagactagt catcaaggcc tgataacgca gtccttttgc
      181 tgccataaat ataagcaaaa ccaacagtga caaagtcaaa acacagtcaa caagcggcat
      241 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
      301 aaaaacattc ccaccggtaa tcccataa
//
LOCUS       DACADA010000467          326 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.481, whole genome shotgun sequence.
ACCESSION   DACADA010000467 DACADA010000000
VERSION     DACADA010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.481"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>326
                     /locus_tag="ISB17_005024"
     CDS             <1..>326
                     /locus_tag="ISB17_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982829.1"
                     /translation="QINGDDATANNNGKTIVDGKDSTGTEIAGNNAVVNQDGTLDVSG
                     GGHGIDITGDSATVDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGNNAV
                     VNQDGTL"
BASE COUNT           93 a           86 c          100 g           47 t
ORIGIN      
        1 caaattaacg gtgacgatgc gaccgcgaac aacaacggca aaaccattgt cgacggcaaa
       61 gattccaccg gcacggaaat cgcgggtaat aacgctgtag tgaatcagga cggtacgctg
      121 gacgtcagcg gcggcggtca cggtattgat atcaccggcg acagcgccac ggtggacaac
      181 gccatcagta acggcggcac cggcacgcag gttaacggtg atgaagccac cgtaaacaat
      241 aacggtaaaa ccaccgttga tggtcagggc tcgaccggca cggaaatcgc gggtaacaac
      301 gcggtggtga atcaggacgg tacgct
//
LOCUS       DACADA010000468          320 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.483, whole genome shotgun sequence.
ACCESSION   DACADA010000468 DACADA010000000
VERSION     DACADA010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.483"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..309
                     /locus_tag="ISB17_005025"
     CDS             <1..309
                     /locus_tag="ISB17_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982830.1"
                     /translation="EKPLLHSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVME
                     NFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG
                     "
BASE COUNT          107 a           65 c           83 g           65 t
ORIGIN      
        1 gagaaaccgc tgctccacag tgatcaagga tggcattacc ggataaaaag ttatcagtcc
       61 gccctggcgg acagagggtt agtgcagagc atgtcgcgca agggcaactg ccttgataat
      121 gcggtaatgg agaacttctt tggtcacctg aaagaagaaa tgtactatcg ccgggactac
      181 agaaatgtag aagagctgga aaatgccgtt aacgaataca taacttactg gaatcaaaaa
      241 agaataaaac tcagtcttgg gggactcagc ccggtagaat accggactga gtatcaaaaa
      301 gccggttaac tgaaactgtc
//
LOCUS       DACADA010000469          315 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.484, whole genome shotgun sequence.
ACCESSION   DACADA010000469 DACADA010000000
VERSION     DACADA010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.484"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>315
                     /locus_tag="ISB17_005026"
     CDS             <1..>315
                     /locus_tag="ISB17_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP1982831.1"
                     /translation="KPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADD
                     GWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLR
                     YVL"
BASE COUNT           79 a           79 c           97 g           60 t
ORIGIN      
        1 aaaccgctgc tgaaatccct ggaaagctgg ctgcgtgaaa agatgaaaac cctgtcgcga
       61 cactcagaac tggcgaaagc gttcgcatac gccctgaacc agtggccggc gctgacgtac
      121 tatgcagatg atggctgggc tgaggcggac aataacatcg ctgaaaatgc gttgcggatg
      181 gtcagtctgg gccgcaaaaa ctacctgttc ttcggttcgg atcatggagg agagcgggga
      241 gcgctgctgt acagcctgat cgggacgtgc aaactgaacg gagtggagcc agaaagctac
      301 ctccgctatg tcctt
//
LOCUS       DACADA010000470          313 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.485, whole genome shotgun sequence.
ACCESSION   DACADA010000470 DACADA010000000
VERSION     DACADA010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.485"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>313)
                     /locus_tag="ISB17_005027"
     CDS             complement(<1..>313)
                     /locus_tag="ISB17_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAP1982832.1"
                     /translation="PSDTIFCRHPSLQYALDSLRNGKGKVNLIKHYSSIESIQQHVPL
                     VRDAEFRALLRHPLARSRVIASQDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVS
                     KD"
BASE COUNT          100 a           58 c           70 g           85 t
ORIGIN      
        1 atctttactt actgacaaag tatgattgtc tatatataaa atcgcggaca tatgactgac
       61 attgtttgcc atcattcgac agaagaaaat atctaaagca aagccaaaat cctgactcgc
      121 gataacgcga ctccttgcaa gaggatggcg aagtaatgct ctgaattccg cgtcccggac
      181 taagggaaca tgctgctgta tggattcaat ggaggagtaa tgtttaatca ggttgacttt
      241 gccttttcca tttctcaatg aatcaagggc gtattgtaat gacggatggc gacaaaaaat
      301 agtgtcagaa gga
//
LOCUS       DACADA010000471          303 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.486, whole genome shotgun sequence.
ACCESSION   DACADA010000471 DACADA010000000
VERSION     DACADA010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.486"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>303
                     /locus_tag="ISB17_005028"
     CDS             <1..>303
                     /locus_tag="ISB17_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1982833.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLA"
BASE COUNT           88 a           77 c           77 g           61 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggc
//
LOCUS       DACADA010000472          297 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.487, whole genome shotgun sequence.
ACCESSION   DACADA010000472 DACADA010000000
VERSION     DACADA010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.487"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           87 a           76 c           67 g           67 t
ORIGIN      
        1 gtgggccacg gaacaggcac accaacgggt agcgccgagc cttctcctaa accaacgttt
       61 ctatgccgcc cttacgatgt aattaaacgc aatgttacgg ggacgaatgg tcacataatt
      121 aaccccatcg cctcgcgaag gacctgcaaa tgaaaaacgg gagaatccag gttggttgtc
      181 aacgatttgg tcatagttat tgattgaatg accagaccca atgtcaaaat tacctgcgta
      241 atgagaaaaa cttgtaccat cctgccgtga aagcagatcg cgccctgtat ccacttt
//
LOCUS       DACADA010000473          293 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.489, whole genome shotgun sequence.
ACCESSION   DACADA010000473 DACADA010000000
VERSION     DACADA010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.489"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            65..140
                     /locus_tag="ISB17_005029"
     tRNA            65..140
                     /locus_tag="ISB17_005029"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           79 a           67 c           70 g           77 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 atacgtcccc ttcgtctaga ggcccaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa cgagagttgt tcgtgagtct ctcaaatttt cgc
//
LOCUS       DACADA010000474          279 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.491, whole genome shotgun sequence.
ACCESSION   DACADA010000474 DACADA010000000
VERSION     DACADA010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.491"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>279
                     /locus_tag="ISB17_005030"
     CDS             <1..>279
                     /locus_tag="ISB17_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HAP1982834.1"
                     /translation="PGIQTQWMSAGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQT
                     DLDGNRARNDQHQFDGYVTYKINDSWLFAWQTTVYTKVNNFKYGNHKKT"
BASE COUNT           92 a           51 c           62 g           74 t
ORIGIN      
        1 ccagggattc agacacaatg gatgtcagca ggaagtcagt ataaccctta tctgaaaatt
       61 tcgtatcagg caacgccaga tcttaatctg gccttacgct atcgctatga ctggaaagct
      121 ttccgacaga cggatttaga tggcaacagg gcgcgtaatg atcagcatca gtttgatggt
      181 tatgtgacat acaaaattaa tgattcatgg ttgtttgcat ggcagaccac ggtatatacg
      241 aaggttaata atttcaaata tgggaatcat aaaaaaaca
//
LOCUS       DACADA010000475          270 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.493, whole genome shotgun sequence.
ACCESSION   DACADA010000475 DACADA010000000
VERSION     DACADA010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.493"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(130..>270)
                     /locus_tag="ISB17_005031"
     CDS             complement(130..>270)
                     /locus_tag="ISB17_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1982835.1"
                     /translation="KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDI
                     RS"
BASE COUNT           64 a           73 c           47 g           86 t
ORIGIN      
        1 gtaaagggga tgacttggga caaaaaaacg ctctctctga ccattctttg tgcatctcct
       61 acccttcaga tgctctcctg catcacaccg cggagagcat gaaaaatatc gtgactctgt
      121 actctcccat caactgcgaa tatcatcata ctcccgtgtt ttatcaaatt cgtgtttcgc
      181 aaatgggcat agtgggataa tttttcgttt ttcccgacgc attttttcca cgactttcgc
      241 aaccagctgt ttaccaatcc cttgtccttt
//
LOCUS       DACADA010000476          267 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.494, whole genome shotgun sequence.
ACCESSION   DACADA010000476 DACADA010000000
VERSION     DACADA010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.494"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           69 a           58 c           75 g           65 t
ORIGIN      
        1 tgaacaaact ttacgatcgc gtgccggctt ccgtctggaa atatgtcacc tgaatcttga
       61 atccgcgcta cgatgactcc ggtgttatct gtgcaccgga agatcgggaa cattaagcag
      121 tataacggca acaaagaatt cgaaatcaaa agggaatggt gtacagaggg gcaggcgttc
      181 aggcattacc ggcctgtatc ttctgaaggg gactcgtatt tgtcggggct gagtattccg
      241 ctcgattcga tcacggattg gcggaat
//
LOCUS       DACADA010000477          266 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.495, whole genome shotgun sequence.
ACCESSION   DACADA010000477 DACADA010000000
VERSION     DACADA010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.495"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>266
                     /locus_tag="ISB17_005032"
     CDS             <1..>266
                     /locus_tag="ISB17_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1982836.1"
                     /translation="TMATQRGYQMGRWLAGRLMKELGLVSCQQPTYRYKRGGHEHVAI
                     PNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPV"
BASE COUNT           60 a           67 c           81 g           58 t
ORIGIN      
        1 acaatggcaa cccagagagg ctaccagatg gggcgctggc ttgctggcag actcatgaaa
       61 gagctggggc tggtcagctg tcagcagccg acttaccggt ataaacgtgg tggtcatgaa
      121 catgttgcta tccctaacta ccttgaacgg cagttcgccg tgaccgagcc aaatcaggtg
      181 tggtgcggtg atgtgaccta tatctggacg ggtaagcgct gggcgtacct cgccgttgtt
      241 ctcgacctgt tcgcaagaaa accagt
//
LOCUS       DACADA010000478          262 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.496, whole genome shotgun sequence.
ACCESSION   DACADA010000478 DACADA010000000
VERSION     DACADA010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.496"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..184
                     /locus_tag="ISB17_005033"
     CDS             <1..184
                     /locus_tag="ISB17_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1982837.1"
                     /translation="MKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLA
                     NLFRVDQMIRQWERSQ"
BASE COUNT           82 a           57 c           71 g           52 t
ORIGIN      
        1 catgaaagcc agcatccgtg ccagggtgga gcacccgttt cgcatcatca agcggcagtt
       61 cggcttcgtg aaagccagat acaaggggct gctgaaaaac gataaccaac tggcgatgtt
      121 attcaccctg gccaacctgt ttcgggtgga ccaaatgata cgtcagtggg agagatctca
      181 gtaaaaaccg gaaataacgc cagaaatggt ggaaaaaata gcctaaatag gctgattcga
      241 tgtgtttgcg ggaaaaaaat cg
//
LOCUS       DACADA010000479          246 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.497, whole genome shotgun sequence.
ACCESSION   DACADA010000479 DACADA010000000
VERSION     DACADA010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.497"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           79 a           56 c           46 g           65 t
ORIGIN      
        1 atttactcac acctgaaaat tataatcgcc ttttcccacc gacgctctta cctgtcgagg
       61 aaaaaccaga aataccgcct gaacgagaaa ttatcctcac cattggcgta ttctttgatg
      121 gcacaggaaa caatttaacc aatacgaatt tacgtatgag tttttgccaa ccagaaacct
      181 atggtctgga tgtccaggat ttggcctcgt ttaataaaca atgtatgagc aagcagggta
      241 aaacgg
//
LOCUS       DACADA010000480          241 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.498, whole genome shotgun sequence.
ACCESSION   DACADA010000480 DACADA010000000
VERSION     DACADA010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.498"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>241
                     /locus_tag="ISB17_005034"
     CDS             <1..>241
                     /locus_tag="ISB17_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982838.1"
                     /translation="IRTHYESPLRMHIDSDILTPSSEPISQFARQLITLLDTSDLSSM
                     LSYCVTQEFTVSCRKISHNCYSTALFIINFATSPIH"
BASE COUNT           65 a           65 c           33 g           78 t
ORIGIN      
        1 tatcagaact cattatgagt ctcctcttag aatgcacatc gatagcgaca tactaacgcc
       61 ttcatcagag cctattagtc aatttgcgcg ccagctcatc accctacttg atacctctga
      121 tttaagttcg atgctgtcat actgtgttac tcaggaattt accgtaagct gtcgaaaaat
      181 atcacacaat tgttattcca ctgccctttt tatcattaac tttgccactt cacccatcca
      241 t
//
LOCUS       DACADA010000481          239 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.499, whole genome shotgun sequence.
ACCESSION   DACADA010000481 DACADA010000000
VERSION     DACADA010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.499"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..>239
                     /locus_tag="ISB17_005035"
     CDS             <1..>239
                     /locus_tag="ISB17_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982839.1"
                     /translation="NKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGD
                     EATVNNNGKTTVDGQGSTGTEIAGNNAVVNQDGTL"
BASE COUNT           69 a           65 c           70 g           35 t
ORIGIN      
        1 aacaaaggcg gtatgacggt taccgatccg gactccatcg gtattctgat cgacggcgac
       61 aaagccatcg tcaacaacga cggcgacaac gccatcagca acggcggcac cggcacgcag
      121 gttaacggtg atgaagccac cgtgaacaat aacggtaaaa ccaccgttga tggtcagggc
      181 tcgaccggca cggaaatcgc gggtaataac gctgtagtga atcaggacgg cacgctgga
//
LOCUS       DACADA010000482          233 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.500, whole genome shotgun sequence.
ACCESSION   DACADA010000482 DACADA010000000
VERSION     DACADA010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.500"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           72 a           50 c           41 g           70 t
ORIGIN      
        1 ttaattgtca gattattcat tactgacaaa accagaataa aatatttgtg acatatatcg
       61 caggttgttc ggtttcctaa ggcatagtac agcatattga gcaatgccag tattcgtcga
      121 ttttgcacat cgttcataat gacctcctat aacataactc cgctggcaat aagaagtttt
      181 gccagctcct ctgacgcctg tagaccacta ataacttgag aacgaaggat ctc
//
LOCUS       DACADA010000483          232 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.501, whole genome shotgun sequence.
ACCESSION   DACADA010000483 DACADA010000000
VERSION     DACADA010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.501"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            48..124
                     /locus_tag="ISB17_005036"
     tRNA            48..124
                     /locus_tag="ISB17_005036"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
BASE COUNT           57 a           53 c           65 g           57 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc at
//
LOCUS       DACADA010000484          225 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.502, whole genome shotgun sequence.
ACCESSION   DACADA010000484 DACADA010000000
VERSION     DACADA010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.502"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            1..225
                     /locus_tag="ISB17_005037"
                     /pseudo
     CDS             1..225
                     /locus_tag="ISB17_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           86 a           54 c           36 g           49 t
ORIGIN      
        1 aatgttaaaa cgccagacac accgaagtgg ccttgtgata accgggaaga acaacaaccc
       61 attaattccc cattctctgg agaaggatac acctctaaac aatttgattt agcgcaacaa
      121 caacgccaac aaattaatgc gtgccatacg aacaccacat atacaaatgc agactactca
      181 aagttgaggc tcaacttgta agtcttatta ataacattga aacga
//
LOCUS       DACADA010000485          220 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.503, whole genome shotgun sequence.
ACCESSION   DACADA010000485 DACADA010000000
VERSION     DACADA010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.503"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           68 a           49 c           48 g           55 t
ORIGIN      
        1 tgatacgaga caacaaaacg gaatgattgc cagccgttag aaaacaccgg acactctcgt
       61 gtaaaaagct accctgtcta tggatatagc gatatcgact catcaagcgt ggactgaatg
      121 atattcagct ccatatgggc ggcaacctgg aggataaaca tgatttcgcc gttggaatca
      181 aggttgttct gcaatatcta ccagactgct tcaatttatc
//
LOCUS       DACADA010000486          211 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.504, whole genome shotgun sequence.
ACCESSION   DACADA010000486 DACADA010000000
VERSION     DACADA010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.504"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
BASE COUNT           58 a           45 c           41 g           67 t
ORIGIN      
        1 ttaagttact cactcttttc tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga
       61 gtccttgttc cactctcgta cagcatcgct ggtcatacgc gaacacgtac aaacagcaat
      121 ggtgaggcgg catcaagagc agggatccgt catttatgca agcattttac cccaaaaacc
      181 ttttatttat aagggtcatt gcgaattatc t
//
LOCUS       DACADA010000487          206 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.denovo.505, whole genome shotgun sequence.
ACCESSION   DACADA010000487 DACADA010000000
VERSION     DACADA010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.denovo.505"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..>206)
                     /locus_tag="ISB17_005038"
     CDS             complement(<1..>206)
                     /locus_tag="ISB17_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982840.1"
                     /translation="CHLCGSALRYHPQYDTELPWFEHTDDRLTEHGQQCPYVRPERRE
                     IQLIKRLQQFVPDALPVVRKASWH"
BASE COUNT           43 a           52 c           58 g           53 t
ORIGIN      
        1 agtgccagct ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt
       61 taatcaactg tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg
      121 tcagcctgtc gtcagtgtgt tcaaaccagg gaagttcagt gtcgtattgc ggatggtatc
      181 tgagtgcact gccacacaag tggcag
//
LOCUS       DACADA010000488        29219 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.047, whole genome shotgun sequence.
ACCESSION   DACADA010000488 DACADA010000000
VERSION     DACADA010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.047"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ISB17_005039"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ISB17_005039"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP1982841.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ISB17_005040"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ISB17_005040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAP1982842.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="ISB17_005041"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="ISB17_005041"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP1982843.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ISB17_005042"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ISB17_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP1982844.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="ISB17_005043"
     CDS             complement(6384..6698)
                     /locus_tag="ISB17_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HAP1982845.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="ISB17_005044"
     CDS             complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="ISB17_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP1982846.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="ISB17_005045"
     CDS             complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="ISB17_005045"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP1982847.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9819..11627
                     /gene="frdA"
                     /locus_tag="ISB17_005046"
     CDS             9819..11627
                     /gene="frdA"
                     /locus_tag="ISB17_005046"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP1982848.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11620..12354
                     /locus_tag="ISB17_005047"
     CDS             11620..12354
                     /locus_tag="ISB17_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP1982849.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12365..12760
                     /gene="frdC"
                     /locus_tag="ISB17_005048"
     CDS             12365..12760
                     /gene="frdC"
                     /locus_tag="ISB17_005048"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP1982850.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12771..13130
                     /gene="frdD"
                     /locus_tag="ISB17_005049"
     CDS             12771..13130
                     /gene="frdD"
                     /locus_tag="ISB17_005049"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP1982851.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13193..14326
                     /gene="blaEC"
                     /locus_tag="ISB17_005050"
     CDS             13193..14326
                     /gene="blaEC"
                     /locus_tag="ISB17_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP1982852.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14415..14948
                     /locus_tag="ISB17_005051"
     CDS             14415..14948
                     /locus_tag="ISB17_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP1982853.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14945..15262)
                     /locus_tag="ISB17_005052"
     CDS             complement(14945..15262)
                     /locus_tag="ISB17_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP1982854.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="ISB17_005053"
     CDS             complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="ISB17_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP1982855.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15695..15820)
                     /gene="ecnA"
                     /locus_tag="ISB17_005054"
     CDS             complement(15695..15820)
                     /gene="ecnA"
                     /locus_tag="ISB17_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP1982856.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15872..16438)
                     /gene="efp"
                     /locus_tag="ISB17_005055"
     CDS             complement(15872..16438)
                     /gene="efp"
                     /locus_tag="ISB17_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP1982857.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16480..17508
                     /gene="epmB"
                     /locus_tag="ISB17_005056"
     CDS             16480..17508
                     /gene="epmB"
                     /locus_tag="ISB17_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP1982858.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17743..18612
                     /locus_tag="ISB17_005057"
     CDS             17743..18612
                     /locus_tag="ISB17_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982859.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18662..19015)
                     /locus_tag="ISB17_005058"
     CDS             complement(18662..19015)
                     /locus_tag="ISB17_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP1982860.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19153..20799)
                     /gene="groL"
                     /locus_tag="ISB17_005059"
     CDS             complement(19153..20799)
                     /gene="groL"
                     /locus_tag="ISB17_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP1982861.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20843..21136)
                     /locus_tag="ISB17_005060"
     CDS             complement(20843..21136)
                     /locus_tag="ISB17_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP1982862.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21412..22668
                     /gene="yjeH"
                     /locus_tag="ISB17_005061"
     CDS             21412..22668
                     /gene="yjeH"
                     /locus_tag="ISB17_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP1982863.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22684..23160)
                     /gene="fxsA"
                     /locus_tag="ISB17_005062"
     CDS             complement(22684..23160)
                     /gene="fxsA"
                     /locus_tag="ISB17_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAP1982864.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23497..24933
                     /gene="aspA"
                     /locus_tag="ISB17_005063"
     CDS             23497..24933
                     /gene="aspA"
                     /locus_tag="ISB17_005063"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAP1982865.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            25051..26352
                     /locus_tag="ISB17_005064"
     CDS             25051..26352
                     /locus_tag="ISB17_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP1982866.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26468..26806
                     /gene="cutA"
                     /locus_tag="ISB17_005065"
     CDS             26468..26806
                     /gene="cutA"
                     /locus_tag="ISB17_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAP1982867.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26782..28479
                     /locus_tag="ISB17_005066"
     CDS             26782..28479
                     /locus_tag="ISB17_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAP1982868.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28516..29091
                     /locus_tag="ISB17_005067"
     CDS             28516..29091
                     /locus_tag="ISB17_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1982869.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         7200 a         7630 c         7366 g         7023 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttctacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcgtt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgatga agtacgccca atcgataccg
     2281 atccgaatgt cctggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtatgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactaggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtggacctg gttgagcaac tggagagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga tgctgaacca gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacctg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcc ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cgcttaccgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tcagagctgg
     3661 ccgaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gaacgggcgc tggaaagtac cgagcaactg gcggaaagta
     3781 gcgccgattt gccaaaagat atcgtcgccc agtttaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgca ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgttactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcacctctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc aaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctaat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccaatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcgccggaa gtcttcctgg
     6121 tggatctgca gcaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagattcacc agctgattct ggctggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggctcacaag caaaactgat agcgcagaaa aaactgccac
     6421 aaccagtagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatcg
     6541 cgagtatcgt catcttcgag atacatcaaa acatcacaga cgttgttaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggag gtaatggtta tccagaggaa cgccacaatg
     6661 aaccagatgc agcacaagac agacatgcgc gcactcatga ggtaacccat agtttaattt
     6721 cgctatagcg catacgaaat ctcactaatt ccctgtcatt acgccctatc gtcaggccga
     6781 cgatagggca tttgattact cagccaccca ctgcggattt tttaccactc tgctgcaata
     6841 gttctggtaa atcgccatcg ctaacagcga gaagaagaca gggccgccaa tcatccacaa
     6901 tgtgctgtcc cagtctccgg cttcaaccac aggttgaatg atggtgaaga cgttcgcaaa
     6961 tgtcaccacc agtacgacaa ccactgtcgc aatcattgcc gacatacgcg ttttgaaaat
     7021 cacaaacggt ctgtcgagat cctgacgtgc tttaaagaac gggaaagcca gcgcgaggaa
     7081 caggtaagga agcgtcatag acacgttcgc catcagcgtc agcttgttaa agaacgccga
     7141 tgcggtaccg ccaccaaacg aaaccagcag gatgaagaca gtaaccaacc cacactgcat
     7201 ccacatggcg atagacggca tccccatcgc attcaggcgc gtcatcggtt ccggccacaa
     7261 tgctttcggc gtcccctgga tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc
     7321 accggtataa gcgaggaaca tcgacagtcc ggtaatacgc gcaaaccata cgcccagcga
     7381 caatgacgct tcaggtgaca aatgcagtgc gttacccagc gtcaccccga ggctcttcat
     7441 cagcacatag gtaatattgc cgaggttaac ggaaccatta cttaatacct gctgccagtt
     7501 tgtgctgacg ccccataaaa atattgccag cgaataaccg attgaaataa caatagcggc
     7561 aaaaacaata cctttggcaa agttcttttc tggattttcc gttttatcaa ccagaccacc
     7621 aaccgcttca attccgccat aggcaaaaat ggcaaatacc acaaacgata gcattgccag
     7681 accggactga taacctgggt tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc
     7741 accatttaat aacaaaatag taatgcttac taacagcaat actaaattca gacacattac
     7801 tgcaataccg cccaccgcag taatgcgggc aattttatta atcccttttg aagcaacgac
     7861 ggtgaccaga atcatccatg ccactgccag cagaccaacc acctgcgtag gctccagtcc
     7921 ggcaatatgc cagtgctggg tcatgtcgct accgtagagg aatgttgaga acggtaccca
     7981 aactttcgcg gaggtactca ccatccagat gatataagag gaaaaccaca taaacgtacc
     8041 aatgaaggca aaacgtggtc cgacactatt attcatccag gaatagatac cgccttcttc
     8101 tttgcgataa gcagctccca tttcagccat cattaaggcg aatggaataa agaataacaa
     8161 tgcagaaaat atataaaagg aaatcgcact ataacccatt aagtaataag ccgatgggct
     8221 attggcaaat ccaaatacag aagtaaagat catcaatatg agtcctatca gactcatctt
     8281 ttttatcgag tgaggcatga aaccatcctt catttcagcg cgtgtataaa acgcgcccac
     8341 tgaactgcac tgcccaacat tgagcgggca gcgtaaaaac caatacagaa ttgatacctg
     8401 gatgatatca aatatccagt cttaaattgt ggcctttagc ggaaaaatga aaacatatgc
     8461 tacagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt tcagaattat
     8521 gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag cgccaacatc
     8581 accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac tttcaaggct
     8641 tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta
     8701 tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca attcatggaa accattcgcc
     8761 agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc ttcggtactg
     8821 atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg tttttctttg
     8881 ccaatatttg gctctacgcc aaaggtaaac agcaattgta gaagcgtgtc gcggtcttct
     8941 tcagtatcgg caacgttgct caaatccagt ttcgctgcca cttcccgcag ttgcgttttg
     9001 tcggcagaga gtgggtcaat ttccagataa cgcaagaaag cttgttgata agaaaggctt
     9061 tctgctgccg gacagtccag cacctgttgc aagagatcgt ccacctcgtt catcaaccgg
     9121 tacatatcat agtgcggtcg ataccactca agcatagtga actcagggtt gtgataacgc
     9181 cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca accggcaacc
     9241 agcaggcgct tcatatggta ttccgggctg gtcattaacc agagattcat cccctgcgaa
     9301 tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc
     9361 tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa acgacggatc
     9421 tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg ctgccaggat
     9481 gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc tactcgcaac
     9541 gcgacggcga gacaaatttt acgcaggaat aacacaacgg tgggcagtga ctaaaaaaag
     9601 cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta gccctcttgc
     9661 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
     9721 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
     9781 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
     9841 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
     9901 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
     9961 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
    10021 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
    10081 accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg agccgtcgcc
    10141 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
    10201 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
    10261 cgcagatcca gcgtttcgac gaacatttcg tgctggatat tctagttgat gatggtcatg
    10321 ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg
    10381 cggtcgttat ggctaccggc ggtgcaggtc gcgtttatcg ttacaacacc aacggcggca
    10441 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
    10501 aattcgttca gtatcaccca accggtctac caggttccgg tatcctgatg accgaaggct
    10561 gccgcggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
    10621 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
    10681 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
    10741 cgcgtggcga tgtggtttat ctcgacctgc gtcaccttgg cgagaaaaaa ctgcatgaac
    10801 gtttgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
    10861 cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact
    10921 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
    10981 gtgcgaaccg tctgggctcc aactcgctgg cggaactggt ggtcttcggt cgtctggccg
    11041 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag
    11101 cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa
    11161 actgggctaa gatccgcgac gaaatgggca tggccatgga agaaggctgc ggtatctacc
    11221 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
    11281 agcgcgtgcg catcaccgac acttccagcg tgttcaacac cgacctgctc tacaccattg
    11341 agctgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
    11401 agtcccgcgg cgcgcaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
    11461 tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
    11521 acgtgaagat tactacgctg ccgccagcta aacgtgttta cggtggcgaa gcggatgcag
    11581 ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg
    11641 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
    11701 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
    11761 ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg ctcctgcggc
    11821 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
    11881 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
    11941 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
    12001 gcggatcagg gtactaacat ccagaccccg gcgcagatgg cgaagtatca ccagttctcc
    12061 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
    12121 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
    12181 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc
    12241 gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
    12301 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
    12361 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
    12421 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcagttccgg ctgtgtggtt
    12481 cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg
    12541 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctggcggc
    12601 agccctactg cacaccaaaa cctggtttga gctggcacca aaagcggcca atatcattgt
    12661 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
    12721 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
    12781 caaatccaaa gcgttctgat gaaccggtat tctggggcct cttcggggcc ggtggtatgt
    12841 ggagcgccat cattgcgccg gtgatgatcc tactggttgg tattctgcta ccgctagggc
    12901 tgtttccagg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
    12961 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgaatgc
    13021 accacgcgat gcacgatctg aaaatccacg tacctgcggg taaatgggtt ttctacggtc
    13081 tggctgctat cctgacagtt gtcacactga ttggtgtcgt tacaatctaa cgcatcgcca
    13141 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ctatgttcaa
    13201 aacgacgctc tgcaccttat taattaccgc ctcttgctcc acatttgccg cccctcaaca
    13261 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa ggatccccgg
    13321 tatggcggta gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
    13381 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
    13441 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
    13501 tcccacaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
    13561 acatctcgca acctacaccg ctggcggcct gccattgcag gtgccggatg aggtgaaatc
    13621 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
    13681 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
    13741 tttgagtttt gagcaggcga tgaaaactcg tgtcttccag ccactcaaac tcaatcatac
    13801 gtggattaat gtaccgtccg cagaagaaaa gaactacgcc tggggatatc gcgaaggtaa
    13861 ggcagtacat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
    13921 tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc cttgatatca atgagaaaat
    13981 acttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
    14041 gggtctgggc tgggaaatgc tggactggcc ggtaaatcct gacatcatca ttaacggcag
    14101 tgacaataaa attgcactgg cagcacgccc cgtaaaaccg attacgcccc caactcctgc
    14161 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
    14221 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
    14281 agcgagagtt accgccgcct ggcagattct caacactcta cagtaaaatt tcatcgggtc
    14341 cgaattttcg gaccttttct ccgcttttcc ttgctgtcat ctacacttag aaaaaaacca
    14401 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
    14461 tcgttgcctg cagttctcct acaccgccgc gtggcgtgac cgtagtgaat aatttcgacg
    14521 ctaaacgcta tctcggcacc tggtatgaga ttgcccgttt tgatcaccgc tttgaacgtg
    14581 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
    14641 ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa gcgtacttta
    14701 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
    14761 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
    14821 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
    14881 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
    14941 gtagttaatg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
    15001 ccagacgcat cgggttagcg gactcaccga gcaacacaat gccggtgatg gccgcgccga
    15061 ctgcgccaat acccgtccac acggcataag ccgtccctac tggtaacgat ttcatcgccc
    15121 aggcaagtag cgccatactg acaatcatcg ccgtcacggt aataacactt ggcgtcaaac
    15181 gactaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
    15241 taactaagat aatccaggac atatcaggct ccggaaaatt ggggccgtcc ccggtgaaaa
    15301 gacgcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
    15361 ggggaatttc aactattacc tatgtaacta tcgcagaaaa aacaaacggc acgacacagc
    15421 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    15481 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    15541 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    15601 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgac
    15661 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcgg gagatggagt
    15721 tgccgaggtg tttgatatct tcgccgaaac ctcgagcggt gttacagccc gtaagcagcg
    15781 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctaacctg aataagtgat
    15841 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
    15901 agcgggtatc cactttgatg acttcgccga tttgtacaaa cagcggaact ttaaccacag
    15961 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
    16021 gatcggtatc cacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
    16081 cattccacag agttacgata cactctgcct gatccagcaa ccatttggcg ttgtcgccaa
    16141 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
    16201 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcacct tcagcggaat
    16261 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
    16321 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
    16381 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
    16441 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
    16501 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
    16561 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
    16621 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
    16681 ccgaacgatc cgcttttgcg tcaggtaatt acctcgcaag atgagtttgt tgtcgcgccc
    16741 ggattctcca ccgacccgct ggaagaacag cacagcgtag tgccaggttt gttgcataaa
    16801 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
    16861 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgca
    16921 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
    16981 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
    17041 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg catcacagat
    17101 gcgctggttg aacgcttttc tcattctacg ctgcaaatct tgctggtgaa tcacattaac
    17161 catgccaatg aggtagatga aacattccgt caggcgatgg ctaagttgcg ccgggtaggc
    17221 gttactttgc tgaaccagag cgttctgtta cgtggtgtga acgataacgc gcaaacgctg
    17281 gcaaacctga gtaatgcgtt gttcgatgcg ggtgtgatgc cgtattacct gcatgtgctc
    17341 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
    17401 cgtgagttgc tgacactggt gtcgggatat ctggtgccta aactggcgcg agaaattggc
    17461 ggcgaaccca gcaaaacacc gctggatctc cagctacgcc agcagtaact gattaaacag
    17521 ggaagtttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttatta
    17581 ctatttatat aaccgacacc aataattaac ctttcttttc tgtttatata gagtgagcac
    17641 ggctatttat gttattttaa caactattac taccaagccc atttatctgg cttataaact
    17701 aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag
    17761 ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt
    17821 aaagaacctc gcaataaaga atcgttgttc tttatgtctg ccatggaatt gcgcgacctt
    17881 ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt
    17941 cagtatgaac aagctcgcga taaagtcatt aagaaaatgg cagaaaatat cccagaaatt
    18001 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
    18061 gatgatcagg gcgaaaatct taccttcgta ttaacactac atgacggtag caaatgtgag
    18121 ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
    18181 aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta
    18241 tatgatgtcg actgccagga tgatggtaat ctggaatatg acacctattc ccaaccggaa
    18301 tggaaacata acttgttcaa ccattatctg gcggtgcttt accgctttaa agatgaaagt
    18361 ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa
    18421 gtggaggcga tcactcgccg aatgcttgat ttcagccccc gactgaaaaa actcgcgggt
    18481 gtgccatgtc aggtctatgt tcgcaccgta gcggcaaata acgcccaacc gctaacccag
    18541 gatcaatgct tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca
    18601 caggcgaaat aatcttatct gttttactac gaggctggaa gtatccagcc tctggatcac
    18661 atcagttcgg gcacttataa acctgaccga taatctggct atccgtcggg acaaaactgg
    18721 acaacattcc ctgcgacggg ctgctgatgc cataaatcac gttaccgccc attgcagccg
    18781 cctggttgcg cagatcgttt gctgcgccgc gcatagaacc gccttcttct ccgtgttgcc
    18841 cggaaagcca gttgctttgc ttacctgttg cagtaccaat cagctggcac tctgcacctg
    18901 gttgctcgtc cacaatgcgt acactctgac cggcagcact caattcgttg ctggagctac
    18961 aacccgccat cagtagcgcg gcaccgacaa tccctgctaa gtattttacg tgcatgttat
    19021 tccccataat caatgagctg gacgccactc gcgtcgtccg tgtctgaatc ttatactaga
    19081 aagatgacca aaagaaaaac ccccagacat ttctgcccgg gggtttgttt atttctgcga
    19141 ggtgcagggc aattacatca tgccgcccat gccacccatg ccgcccatac cgccagcagc
    19201 gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc atgcactcgg tggtgatcat
    19261 caggccagcc acagaagccg cgtactgcag agcagaacgg gttactttgg ttggatccag
    19321 gatacccata tcgatcatgt tgccgtattc ttcagttgct gcgttgtaac cgtagttgcc
    19381 gtcgccgcct ttaacggtgt tagcaacaac agacggttct tcgccgcagt tcaggacgat
    19441 ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg atacccacgt tctggtcttc
    19501 gttctgacca cgcaggtcag ccagtttaga cgctacgcgg atcagcgcaa caccaccacc
    19561 agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc agggcgtctt caacgcgtgc
    19621 ttttttctct ttcatttcaa cttcggtagc agcaccaact ttgataactg caacgccgcc
    19681 tgccagtttc gctacgcgct cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc
    19741 aatctgctga cggatctgag caacacggcc ctggattgca gcttcttcac ccacgccatc
    19801 gatgatggtg gtggtgtctt tgttgatcac aacacgttta gcctgaccca ggtcttccag
    19861 ggttgctttt tccagctcca taccgatctc ttcagagatt acggtaccgc cagtcagggt
    19921 tgcgatatcc tgcagcatag ctttacgacg atcgccgaag cccggagctt taaccgcagc
    19981 aactttcacg atgccacgca tggtgttaac aaccagagtt gccagtgctt cgccttcaac
    20041 atcttcagcg atgatcagca gcggtttacc tgctttggca acagcttcca gaaccggcag
    20101 catttcgcgg atgttggaga ttttcttgtc agccagcagg atgaacgggc tttccagttc
    20161 tactgcgcca gtttccggct tgttgatgaa gtaaggagac aagtagccac ggtcgaactg
    20221 cataccttca accacgtcca gttcgtcctg cagaccggta ccgtcttcaa cggtgataac
    20281 gccttcttta ccgactttgt ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga
    20341 gttagcagag atggtaccaa cctgagcaat cgctttagag tcagagcacg gtacggacag
    20401 cgctttcagt tcttcaactg cagcggtaac agctttgtcg ataccacgtt tcaggtccat
    20461 cgggttcatg cccgcagcaa cagctttcag accctcagtg atgatagcct gagccagtac
    20521 ggttgcagtg gtggtaccgt cgcctgcagc gtcattcgct ttagaggcaa cttctttcac
    20581 catctgcgca cccatatttt cgaacttgtc ttccagttcg atttcacgag caacggaaac
    20641 accatctttg gtgatggtcg gtgcaccgaa agatttatcc agaactacgt tacggccttt
    20701 aggaccgagg gtaactttca ctgcatctgc cagtacgttt acgccgcgca gcattttcac
    20761 acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt atctttattc cttaaattcg
    20821 tatgttcagt gtcgtgcgcg gattacgctt caacaattgc cagaatgtcg ctttcggaca
    20881 tgatcaacac ttcttcattg tcgatcttct cagatttcac accgtagcca tcgttgaaaa
    20941 taacgatgtc gccaactttc acatccagcg gcttcacttc gccattttca aggatacggc
    21001 cattgccgac agccagcact tcgccgcggg tggatttagc cgctgcagag ccggtcagaa
    21061 cgatgccgcc agcagattta gtttcaactt ctttacgctt gacgatcacg cgatcatgca
    21121 atggacgaat attcattgat aactctcctt tgagaaagtc cgtatctgtt atgggtgacg
    21181 ccggagctta cgtggtttcc cggctggtga ccagagaaat ggggatgagg cttcgcccct
    21241 tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa tcgggtgaaa accctgattc
    21301 acctcacatt tcatcgctat ttcttcatca ccgtcataag caaatctgat tgtgctacca
    21361 tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg gacatcaacc catgagtgga
    21421 ctcaaacaag aactggggct ggcccagggc atcggcctgc tatcgacgtc attattaggc
    21481 actggcgtgt ttgccgttcc tgcgttagct gcgctagtag caggcaataa cagcctgtgg
    21541 gcgtggcccg ttttgattat cttagtgttc ccgattgcga ttgtgtttgc gattctgggt
    21601 cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg gtatggcgtt tggttcgcgg
    21661 cttgagcgag tcaccggctg gttgttttta tcggtcattc ccgtgggttt gcctgccgcg
    21721 ctacaaattg ctgccggatt cggtcaggca atgtttggct ggcatagctg gcaactgttg
    21781 ttggcagaac tcggtacgct ggcgctggtg tggtatatcg gtactcgcgg tgccagttcc
    21841 agtgctaatc tacaaacagt tattgccgga cttatcgtcg cactgattgt cgctatctgg
    21901 tgggcgggcg atatcaaacc tgcgaatatc cccttccctg cgccaggaaa tatcgaactt
    21961 accgggttat ttgctgcgtt atcagtgatg ttctggtgtt ttgtcggtct ggaagcattt
    22021 gcccatcttg cctcggaatt taaaaatcca gagcgtgatt ttcctcgtgc tttgatgatt
    22081 ggcctgctgc tggcaggatt agtctattgg ggctgtacgg tagtcgtctt acacttcgac
    22141 gcctatggtg aacaaatggc ggcggcagca tcgcttccca aaattgtagt gcagttgttc
    22201 ggtgtaggag cgttatggat tgcctgcgta attggctatc tggcctgctt tgccagtctc
    22261 aacatttata tacagagctt cgcccgcctg gtctggtcgc aggcgcaaca taatcctgac
    22321 cactacctgg cacgcctctc ttctcgccat atcccgaata atgccctcaa tgcggtgctc
    22381 ggctgctgcg tggtaagcac gttggtgatt catgctttag agatcaatct ggacgctctt
    22441 attatttatg ccaatggcat ctttattatg atttatctgt tatgcatgct ggcaggctgt
    22501 aaattattgc aaggacgtta tcgactactg gcggtggttg gcgggctatt atgcgttctg
    22561 ttactggcaa tggtcggctg gaaaagtctc tacgcgctga tcatgctggc ggggttatgg
    22621 ctgtttctgc caaaacgaaa aacgccggaa aatggcataa ccacataatc cggcgtttcg
    22681 acattaatcc tggcgatcgt ctttatgatc aaggcggtcg cggtcatcat cctttcgctg
    22741 gtactcacca tcaaaagtat taccgccacc ggtcccggcg ctaaaaccgc cgcctggcat
    22801 gcgagaaaag cgcaaatgcg gcatcaactt cactgtcaga tgcttttgca ccggcggcaa
    22861 taaaagcaga agaccgagga agtcggtgaa aaagcccggt aataaaagca gcaaaccggc
    22921 aatgatcagc gaaacacttt taatcatctc cgccgctggg ttttcacccg ccgccatttt
    22981 ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt accagtgaca taccgataac
    23041 tgacgtgaat ataaccagca cgagggtcag caataccccc aatacatgag caacctgaat
    23101 aaagattgaa atctcaatat agacataaag gaaaatggca ataaaaggta accagcgcaa
    23161 aggtttctcc tgtaatagca gccggttaaa cccggctacc tgaatgggtt gcgaatcgcg
    23221 tttagcttat attgtggtca ttagcaaaat ttcaagatgt ttgcgcaact atttttggta
    23281 gtaatcccaa agcggtgatc catttcacaa attaataatt aaggggtaaa aaccgacact
    23341 taaagtgatc caggttacgg tagaaatcct caagcagcat atgatctcgg gtattcggtc
    23401 gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc acatatattc tgtgtgttta
    23461 aagcaaatca ttggcagctt gaaaaagaag gttcacatgt caaacaacat tcgtatcgaa
    23521 gaagatctgt tgggtaccag ggaagttcca gctgatgcct actatggtgt tcacactctg
    23581 agagcgattg aaaacttcta tatcagcaac aacaaaatca gtgatattcc tgaatttgtt
    23641 cgcggtatgg taatggttaa aaaagccgca gctatggcaa acaaagagct gcaaaccatt
    23701 cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg aagtcctgaa caacggaaaa
    23761 tgcatggatc agttcccggt agacgtctac cagggcggcg caggtacttc cgtaaacatg
    23821 aacaccaacg aagtgctggc caatatcggt ctggaactga tgggtcacca gaaaggtgaa
    23881 tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc agtctactaa cgacgcctac
    23941 ccgaccggtt tccgtatcgc agtttactct tccctgatta aactggtaga tgcgattaac
    24001 caactgcgtg aaggctttga acgtaaagct gtcgaattcc aggacatcct gaaaatgggt
    24061 cgtacccagc tgcaggacgc agtaccgatg accctcggtc aggaattccg cgctttcagc
    24121 atcctgctga aagaagaagt gaaaaacatc caacgtaccg ctgaactgct gctggaagtt
    24181 aaccttggcg caacagcaat cggtactggt ctgaacacgc cgaaagagta ctctccgctg
    24241 gcagtgaaaa aactggctga agtcactggc ttcccatgcg taccggctga agacctgatc
    24301 gaagcgacct ccgactgcgg cgcttatgtt atggttcacg gcgcgctgaa acgcctggct
    24361 gtgaagatgt ccaaaatctg taacgacctg cgcttgctct cttctggtcc acgtgccggc
    24421 ctgaacgaga tcaacctgcc ggaactgcag gcaggctctt ccatcatgcc agctaaagta
    24481 aacccggttg ttccggaagt ggttaaccag gtatgcttca aagtcatcgg taacgacacc
    24541 actgttacca tggcagcaga agcaggtcag ctgcagttga acgttatgga gccagtcatt
    24601 ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg cttgctacaa cctgctggaa
    24661 aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg aaggttacgt ttacaactct
    24721 atcggtatcg ttacttacct gaacccgttc atcggtcacc acaacggtga catcgtgggt
    24781 aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg ttctggaacg cggtctgttg
    24841 actgaagcgg aacttgacga tattttctcc gtacagaatc tgatgcaccc ggcttacaaa
    24901 gcaaaacgct atactgatga aagcgaacag taatcgtaca gggtagtaca aataaagaag
    24961 gcacgtcaga tggcgtgcct tttttcttgt gagcagtaac ttaaaaataa caatttaata
    25021 tcaacttgtt aaaaaacaag gaaggctaat atgctagttg tagaactcat catagttttg
    25081 ctggcgatct tcttgggcgc cagattgggg ggaataggta ttggttttgc aggcggattg
    25141 ggggtgctgg ttcttgccgc tattggcgtt aaacccggta acatcccgtt cgatgtcatt
    25201 tccattatca tggcggttat cgccgctatt tctgccatgc aggttgctgg cggtctggac
    25261 tatctggttc atcagacaga aaagctgctg cgccgtaacc cgaaatacat cacgatcctc
    25321 gcaccgatcg tgacctattt cctgactatc tttgctggta ctggcaacat ctctctggcg
    25381 acactgccag ttatcgctga agttgcgaag gaacaaggcg ttaaaccttg ccgtccgctg
    25441 tctactgcag tggtatccgc gcagattgcg atcaccgcat cgccaatctc agcggcagtg
    25501 gtttacatgt cttccgtgat ggaaggtcat ggcatcagct acctccatct gctctccgtg
    25561 gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct tcctggtcac tatgctgttc
    25621 aactccaaac tctctgacga tccgatttat cgcaagcgtc tggaagaggg cctggttgaa
    25681 ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc
    25741 ctgctgggcg tagttggcgt ggttatctat gcaatcatca acagcccaag catgggtctg
    25801 gttgaaaaac cactgatgaa caccaccaac gcaatcctga tcatcatgct cagtgttgca
    25861 actctgacca ccgttatctg taaagtcgat accgacaaca ttctcaactc cagcaccttc
    25921 aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg cgtggctggg cgatactttc
    25981 gtttccaaca acatcgactg gatcaaagat accgctggtg aagtgattca gggtcatccg
    26041 tggctgctgg ccgtcatctt cttctttgct tctgctctgc tgtactctca ggctgcaacc
    26101 gcaaaagcac tgatgccgat ggctctggca ctgaacgttt ctccgctgac cgctgttgct
    26161 tcttttgctg cggtgtctgg tctgttcatt ctgccgacct acccgacgct ggttgctgcg
    26221 gtacagatgg atgacacggg tactacccgt atcggtaaat tcgtcttcaa ccatccgttc
    26281 ttcatcccgg gtactctggg tgttgccctg gccgtttgct tcggcttcgt gctgggtagc
    26341 ttcatgctgt aatgacccat cgcggggcgt tcacgccccg ctttctttcc cgccgactaa
    26401 catcctttcc ccgtccgttg tatagtgacc tctctcttgc agttccatct gttcttgcga
    26461 ggtgtttatg cttgatgaaa aaagttcgaa taccacgtct gtcgtggtgc tatgtacggc
    26521 accggatgaa gcgacagccc aggatttagc cgccaaagtg ctggcggaaa aactggcggc
    26581 ctgcgcgacc ttgatccccg gcgctacctc tctctattac tgggaaggta agctggagca
    26641 agaatacgaa gtgcagatga ttttaaaaac taccgtatct caccagcagg cactgctgga
    26701 atgcctgaag tctcatcatc catatcaaac cccggaactt ctggttttac ctgttacaca
    26761 cggagacaca gattacctct catggctcaa cgcatcttta cgctgatcct gctactttgc
    26821 agcacttccg tttttgccgg attattcgac gcgccgggac gttcacaatt tgtccccgcg
    26881 gatcaagcct ttgcttttga ttttcagcaa aaccaacatg accttaatct gacctggcag
    26941 atcaaagacg gttactacct ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa
    27001 attgctgacg tgcagctgcc gcaaggcgtc tggcatgaag atgagtttta cggcaaaagc
    27061 gagatttacc gcgatcggct gacgcttccc gtaaccatca accaggcaag tgcgggagca
    27121 acgttaactg tcacctacca gggctgtgct gatgccggtt tctgttatcc gccagaaacc
    27181 aaaaccgttc cgttaagcga agtggtcgcc aacaacgaag cgtcacagcc tgtgtctgtt
    27241 ccgcagcaag agcagcccac cgcgcaattg cccttttccg cgctctgggc gttgttgatc
    27301 ggtattggta tcgcctttac gccatgcgtg ctgccaatgt acccactgat ttctggcatc
    27361 gtgctgggcg gtaaacagcg gctttccact gccagagcat tgttgctgac ctttatttat
    27421 gtgcagggga tggcgctgac ttacacggcg ctgggtctgg tggttgccgc cgcagggtta
    27481 cagttccagg cggcgctaca gcacccatac gtgctcattg gcctcgccat cgtctttacc
    27541 ttgctggcga tgtcaatgtt tggcttgttt actctgcaac tcccctcttc gctgcaaaca
    27601 cgtctcacgc tgatgagcaa tcgccaacag ggcggctcac ctggcggtgt gtttattatg
    27661 ggggcgattg ccggactgat ctgttcacca tgcaccactg caccgcttag cgcgattctg
    27721 ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg gcacgcttta tctttatgcg
    27781 ctgggcatgg gcctgccgct gatgctaatt accgtctttg gtaaccgctt gctgccgaaa
    27841 agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg
    27901 gtcttcctgc tggagcgagt gattggtgat atatggggat tacgcttgtg gtcggcgctt
    27961 ggtgtcgcat tctttggctg ggcctttatc accagcctac aggccaaacg cggctggatg
    28021 cgcgtggtgc aaataatcct gctggcagcg gcattggtta gcgtacgccc acttcaggat
    28081 tgggcatttg gtgcaacaca taccgcgcaa actcagacgc atctcaactt tacacaaatc
    28141 aaaacggtag atgagttaaa tcaggcgctc gttgaagcca aaggcaaacc agtgatgtta
    28201 gatctctatg ccgactggtg cgtcgcctgt aaagagtttg agaaatacac cttcagcgac
    28261 tcgcaggtgc aaaaagcgtt agcagatacg gtcttacttc aggccaacgt cactgccaac
    28321 gacgcacaag atgtggcgct gttaaagcat cttaatgtcc ttggcctacc gacaattctc
    28381 ttttttgacg gacaaggcca ggagcatcca caagcacgcg tcacgggctt tatggatgct
    28441 gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag
    28501 acggaggaaa atagcgtgca acgtgaagat gtactgggag aagccctgaa attattagaa
    28561 ttacaaggga ttgccaacac cacgctggag atggttgctg aacgtgtgga ttatccactg
    28621 gacgagctac gccgcttctg gccagacaaa gaggcaatcc tctacgatgc gctgcgttat
    28681 cttagccaac agatagatgt ctggcgtcgt cagctgatgc tggacgaaac gcaaaccgcc
    28741 gaacaaaagt tgctggcacg ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat
    28801 ccgggctgtc tgtttatcgc tgcctgtacg ttttatcccg atcctggcca ccctattcat
    28861 caactggccg atcagcaaaa aagcgcggcc tacgatttca cccacgaact gttaaccacg
    28921 ctggaagttg acgatccggc gatggtagca aagcagatgg aactggtgct ggaaggctgt
    28981 ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg acaccgcaca tcggctggcg
    29041 gaagatatcc tgcgcttcgc ccgctgccgt cagggtggtg cactgacctg acagaaacac
    29101 agaaaagaag cgatttgccg caatcttaag cagttgaatc gcttttactg aaattaggtt
    29161 gacgagatgt gcagattacg gtttaatgcg ccccgttgcc cggatagctc agtcggtag
//
LOCUS       DACADA010000489        17914 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.090, whole genome shotgun sequence.
ACCESSION   DACADA010000489 DACADA010000000
VERSION     DACADA010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.090"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            318..1634
                     /gene="fliD"
                     /locus_tag="ISB17_005068"
     CDS             318..1634
                     /gene="fliD"
                     /locus_tag="ISB17_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1982870.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            1657..2049
                     /gene="fliS"
                     /locus_tag="ISB17_005069"
     CDS             1657..2049
                     /gene="fliS"
                     /locus_tag="ISB17_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1982871.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            2054..2365
                     /locus_tag="ISB17_005070"
     CDS             2054..2365
                     /locus_tag="ISB17_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAP1982872.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            2362..3423
                     /locus_tag="ISB17_005071"
     CDS             2362..3423
                     /locus_tag="ISB17_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAP1982873.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            3431..3898
                     /locus_tag="ISB17_005072"
     CDS             3431..3898
                     /locus_tag="ISB17_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAP1982874.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            3918..4634
                     /locus_tag="ISB17_005073"
     CDS             3918..4634
                     /locus_tag="ISB17_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAP1982875.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            4647..5510
                     /gene="motA"
                     /locus_tag="ISB17_005074"
     CDS             4647..5510
                     /gene="motA"
                     /locus_tag="ISB17_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1982876.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            5513..6436
                     /gene="lafU"
                     /locus_tag="ISB17_005075"
     CDS             5513..6436
                     /gene="lafU"
                     /locus_tag="ISB17_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP1982877.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            6507..7562
                     /gene="dinB"
                     /locus_tag="ISB17_005076"
     CDS             6507..7562
                     /gene="dinB"
                     /locus_tag="ISB17_005076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP1982878.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            7559..8011
                     /locus_tag="ISB17_005077"
     CDS             7559..8011
                     /locus_tag="ISB17_005077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1982879.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            8189..9340
                     /locus_tag="ISB17_005078"
     CDS             8189..9340
                     /locus_tag="ISB17_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAP1982880.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            9337..9951
                     /locus_tag="ISB17_005079"
     CDS             9337..9951
                     /locus_tag="ISB17_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAP1982881.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(10007..11464)
                     /gene="pepD"
                     /locus_tag="ISB17_005080"
     CDS             complement(10007..11464)
                     /gene="pepD"
                     /locus_tag="ISB17_005080"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP1982882.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            11725..12183
                     /gene="gpt"
                     /locus_tag="ISB17_005081"
     CDS             11725..12183
                     /gene="gpt"
                     /locus_tag="ISB17_005081"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP1982883.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            12275..13519
                     /gene="frsA"
                     /locus_tag="ISB17_005082"
     CDS             12275..13519
                     /gene="frsA"
                     /locus_tag="ISB17_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP1982884.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            13577..13978
                     /locus_tag="ISB17_005083"
     CDS             13577..13978
                     /locus_tag="ISB17_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP1982885.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(14017..15072)
                     /gene="phoE"
                     /locus_tag="ISB17_005084"
     CDS             complement(14017..15072)
                     /gene="phoE"
                     /locus_tag="ISB17_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP1982886.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            15361..16464
                     /gene="proB"
                     /locus_tag="ISB17_005085"
     CDS             15361..16464
                     /gene="proB"
                     /locus_tag="ISB17_005085"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP1982887.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            16476..17729
                     /gene="proA"
                     /locus_tag="ISB17_005086"
     CDS             16476..17729
                     /gene="proA"
                     /locus_tag="ISB17_005086"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP1982888.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         4421 a         4460 c         4880 g         4153 t
ORIGIN      
        1 gattgccgga tgcggcgtga acgccttatc cggcctacaa atgggcacac gcataaaaac
       61 aggcgcggac tcgtaggcct gataagacgc gtcagcgttg catcaggcac tgtgcaccga
      121 tagccggatg cggcgtaaac gccttatccg gccacgaaca acaccgcctg tgggcggtgt
      181 tgtgctttct gcggcaggct tttttccgcc agaccggaaa cgtcatttcc gcctgttttt
      241 caatctattg attattcaca taaaaaatat tggcacgcct tttgccaata acctgacatt
      301 gttatccgga gtcatcaatg atcaacccaa gaaccattgc gcaagaaatc gcgtatgcgg
      361 atgtcgctac tcaggcgacg aatttgcagg agaagcagag tgagctggat gctgaaagca
      421 gcggcctgga ctcgctcagc tcggcgttga gcgatttcca gagcgccgtt gacgcgctga
      481 acagcgatac cgacggcccg gtgacttttg ccgccaccag caataatgac tcggcgaccg
      541 tttccgccaa ttcccaggcg caggcgggaa gctactcgtt tttcgttgag caactggcgc
      601 aggggcagca aaccacgttc agtatgggcg acgacgcctt ttccgctacc ggtacttttg
      661 aactgacgat gggcgacagc actatggata tcgatctgtc ggcggcagat cagaacggtg
      721 acggtgacgg ttttatcgac gccagcgagc tggtgaatgc gattaacgac tccgatgaca
      781 atccgggcgt gtcggcggca ctggtaaaaa ccgacggcac caccacgatt atgctcacct
      841 ccgatagcac cggggcgcag agcgcgtttt cggtgagcgt aacggggcat gatgccagca
      901 acgatagcac cagtgcgccc gttgccacgg atgtttcctc cgctcaggac gcgattattc
      961 atctcggtag cgccagcgga ccggctatca ccaacagcag caacacgttt gatgatgtga
     1021 tccccggtgt caccatgact tttaccgaag tcagcgattc tgacagcgat ctcaccacct
     1081 ttaacatcag tgaagattcc agcgccagcc aggagaaagt acagaccttt gtcgatgcct
     1141 ataacacctt gattgatacg gttgattcgc taaccaccca cggtgatgac agtagtagcg
     1201 ccggggtctt tgcgggggat gctggactca gctcactggc aaaccagctc gatgacatcg
     1261 cccatgccag ctacaacggc gtgtcgattg tcgactatgg catcacgctc gattctcacg
     1321 gccacttaca gattgactcc gaccagttca acgacgcgat gaagagcgat cccgacggcc
     1381 tgacctctat tttcgttggc gacaacagca tggtggcgca gatggacgac ctgattaaca
     1441 cctacacgga ctccagcaac ggcatcatca ccctgcgcca gcagaacatt gatgatcaga
     1501 tgagcaaaat tcaggacgaa ggcgatcagc ttaccgatac ctataacgct aactacgacc
     1561 gttatctcga ggagtacacc aatacgttgg ttgaggtgta caccatgaaa gccagtatgg
     1621 cggcatttgc gtaattcaga aggaatatcc ttaaagatgt accaagtaca ggacggttat
     1681 tcgcaataca aagagatcga tctcgcggcg cgtaccgcgg cggcatcgcc tcttgaactg
     1741 gtgctggtgc tgttttctgg cctgatggac gagctggaac gcgccagaag ccacattgaa
     1801 gggcggcgtt tcgagaagaa agcccagagc atcaataaat gcatcgacat tctcaatgcg
     1861 ctcaccagtt cgctggaatt tgaaaccggc ggcgagctgg tggtcaacct ttcgcgcctc
     1921 tatgaccact gtgtgtatcg cttgtatgaa gccagcggcg aactgtcggc agagaaaatc
     1981 gatgaagtga tgctgatctt aagcaacctg cgcgaaggct gggaagggct ttcgggcaag
     2041 ctgggttaag acgatggaaa aacaacgcag gcaacttttt gcactggtcg aagcgatgaa
     2101 tgacgccctc gacaagcaac gctggcgacg ccttccggca ctgtatcagc agttgatgcg
     2161 ccagtttcac gattacgcgg cggcggaaac cgacgcggcg cagttggatg cggtgaaggc
     2221 acaactgtac ggcgcgttta atgccctgat taaacggcgc gagcagcgcg cagaagtgtt
     2281 gaaagcgcgt atggaacagc atcagcggca tcaggaaggc gtactggcct actcaattgt
     2341 taatttactt tcggagaagt catgaatccg gcattgcttg ccacgctcgg aacgcttgcg
     2401 gaaaccgctt cgttgaaggc ggatatttta ccgccggtga gcggtgaaaa cgcgcccgtg
     2461 tttaccctgc cgaaaatggt ggtcgcggcg gtggcggagc gcgttcatag cgctaaaacc
     2521 agtcagcaac aggcgacgcg cccgcaggag aacgatccgg tggcgatgca ggcgctaatg
     2581 gcgctgttac ttccgcaacc tgccgcgccg catcaggaca cgccgcagcc gcgaaacgtc
     2641 gcaacatcgc cggttatcca gcaattgacg aaagcggtgg tgcaaaacgc gccgcaacgc
     2701 ccgacgcagc agcaggaact cacgtcgttg ccgccgcagt tgcaggaact gatcggtcag
     2761 ttgccgcagg cgaaacccga acagcaggcc agactggcga cttacgccag tgaagattta
     2821 catgccattg cgccgacgca gccgcgcgtc tcaacaccgc cagctcgccc gaaacctgaa
     2881 ctaacccgtg tgaccgcgcg cccgcaggtc gagcgtaaaa cggaaaaagt gccggacagc
     2941 gaaccggtta ttgcgcgtgc ggtgttgcag gttaagacgc cagagttggt cagcgatcat
     3001 caggagattg tcgccaaacc agccacgctt tcgatggacg aactgggcga aaaactgacg
     3061 acgctgttga aagatcagat ccactttcag ctcaacaaac aacagcagat ctccaccatt
     3121 cgtctcgatc caccgtcgct tggcaagctt gagatcgccg tacagttgga taacggcaaa
     3181 ctgacggtcc atatcggtgc gaatcaaagc gaagtctgcc gcgcattgca gcagtttagc
     3241 gacgatctcc gccagcatct gacggcgcaa aattttatgg aggtgaacgt acaggtttcc
     3301 tccgaagggc agtcgcagca gcaacaacag tcgggccatc agcaggaaga ggtgagtgcc
     3361 gccttacagc ttgatgatgc gcctcaattt caacagaacg aatccgtttt gatcaaagtg
     3421 taaaggcaat atgaagaaaa tcgtggtggc aggcgtggtc agcgccgttc tggcgttggt
     3481 ggtcggcgct ggagcaggat ggagtgtctg gcatcattat gccggaaaag gaaaaccgac
     3541 taccgccgcg caaacggaat cggtggaagc tctggatgag agcaagagcg tatttgtgac
     3601 gctgccggaa accattgtca ctttgcatga caacaacggc gcggatcatt atttgtcggc
     3661 ggaattagtg atggtggtcg ccagtgacaa agaggcggaa aaaataaagc atcaggaacc
     3721 gctgtatcaa agtattgcgg ttgaatgcct gacggaaatg aaatttgaag atttgcgcgg
     3781 catgaaaata tctgccatcc gcaaactgat ttctgacgcg ttaaagaaag atcttcagcg
     3841 tcgcaaaatg accgcaccgt ataaggattt gctggtgaaa aaagtggtct tccagtaatc
     3901 accaggcgga tataaacatg ctacaggatg tattcgagtc agaagaattt gcagccacgc
     3961 tggtattaac tccgcagcag gagagccact acttgcaggc ctatttaccg ttggtgcgta
     4021 aagtggtgcg gcaacttgcc ccgcagtgta attgcattat tgaccgtcag gatatggaac
     4081 aaacggcgtt aatggggctg ttgaacgcca ttcgtcgcta tgggctgccg gacgaaggtt
     4141 ttgcgggcta tgccgtgcac cgtattcgcg gtgcgatcct ggatgagctg cgcgctctcg
     4201 actggcgacc gcgccagctg cgacagaaat atcatcagat gaaagacctg attcgcgaaa
     4261 cgcgtaaaaa actggggcac gaaccggagt ggcaggagct ggctgaggaa gggatctcgc
     4321 atgaagatta tctcgaatat cagcaactgg aaggggcgga aacgctcgcc agcctggacg
     4381 aactgctgaa cggcgaaggg ccaggcattc cgcttgctgg ccgggaactg gaagatcagt
     4441 ttgtcactca gcaaatgttg caacacgcgc tttcacaatt aagcgaaaaa gagcaactga
     4501 tcctctccat gtattaccag catgagatga atctcaaaga gattgcgctg gtattagggc
     4561 taaccgaggc ccgaatttgt caactgaata aacagatcgc gcagaaaatt cgtgattttg
     4621 tctatccgaa ttaaagtgaa gaaatcatgc aaaaaatatt aggtttactg gtggtgctcg
     4681 gctgcgtatt tggcggttat tttgaagcgg gcgggcaatt agtggctatc tggcaaccgg
     4741 cggaaatgat cattattttg ggtgccggtt tcggggcgat gattattggt aacccgaaac
     4801 atgtgctgaa agagattgcc catcaaatta aaggcgtgat cagtaaaaaa cagctcgggc
     4861 cggagtttca gcgtcagcta ttaatgtgcc tgtatgagtt gctcgaaatg gtgcaaaacg
     4921 gcggtctgcg gatgttggat cagcatattg agcagccgga agagagcacc attttccaga
     4981 aatacccgct ggtgttaacc cagaagcggc tggtgacctt tattgctgat aactttcgcc
     5041 tgatggcgat gggtaaaatc gacgcccatg agctggaagg cattctcgat caggaactgg
     5101 acaccgcgga agagtccctg ttaacgccgt cgcgctcgct gcaacgtacc gccgaagcga
     5161 tgccgggatt tggtatttgc gcggcggtgc tgggcatcat catcaccatg cagtcgattg
     5221 atggatctat cgctctgatt ggcctgaaag tggcggcggc tctggtgggg acgtttttag
     5281 gcgtgtttat ctgctactgc ctgatggacc cgctagccaa cgccatggaa cagcaggcgc
     5341 gggcggaaca ttctttgctg gaatgcgtgc gcactgtgct ggtggcgcag gcaggcggca
     5401 aaccgacgct gctggcggtg gacgccgggc gtaagctgct gcatctggcg tccaaaccga
     5461 ccttcgccaa cctcgacgcg tgggtgaatg cgatgctgga gcaagaataa gcatgagaaa
     5521 gacgggcaac caccgcgaac gtggggcaaa aaccaccatc gtccggcggc agatcaaaaa
     5581 gaatcatgcc gggcatcacg gcggggcgtg gaaggtggcg ttcgccgact ttactttggc
     5641 aatgatggcg ctatttatga cgctgtggat tgtcaacagc gtcagtaaat ccgagcgtga
     5701 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggt tgtcgccgtt
     5761 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagagac
     5821 ggagaaaaaa gcgcgcgacg tcaacgagaa aacggcgctg ctgaagaaga aatccgccac
     5881 cgagcttggc gagctggcaa ccagtatcaa taccatcgcc cgtgatgcgc atatggaagc
     5941 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     6001 ccgcaatatg ttcgaacgcg gcagcgcgaa gattatgccg ttctttaaaa ctttgctggt
     6061 ggagcttgcg ccagtgttcg actcgctcga taacaaaatt attattaccg ggcataccga
     6121 tgcgatggcc tacaaaaaca atatctacaa caactggaac ctttcgggcg atcgcgcgct
     6181 ttcagctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     6241 tgcaatggcg gaccagatgc tgctggatgc caaaaatccg caaagcgcgg gcaaccggcg
     6301 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     6361 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     6421 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaaccctgaa atcactgtat
     6481 actttaccag tattgagagg tgagcaatgc gtaaaatcat tcatgtggat atggactgct
     6541 ttttcgcagc ggtggagatg cgcgacaatc ccgccctgcg cgatatccct attgctattg
     6601 gcggcagccg cgaacgacgg ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg
     6661 gcgtacgtag cgctatgccg acagggatgg cgctcaaatt atgcccgcat ctcaccttgc
     6721 ttccagggcg ctttgacgcc tacaaagaag cctcaaatca tatccgcgaa attttctcgc
     6781 gctatacctc gcgcattgaa ccgttatcac tggatgaagc ctatctcgat gtcaccgata
     6841 gcgtccattg ccacggttct gcgaccctca tcgcccagga aatccgccag acgatcttca
     6901 acgagctgca actgacggcg tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg
     6961 cctccgacat gaataaaccc aacggccagt ttgtgattac gccggcagaa gttccgacat
     7021 ttttacaaac cttaccgctg gcaaaaattc ccggcgtcgg caaagtctcg gcggcaaaac
     7081 tggaagcgat ggggctgcgg acctgcggtg atgtgcaaaa gtgtgatctg gtgatgctgc
     7141 ttaaacgctt tggcaaattt ggccgcattt tgtgggagcg tagtcagggc attgacgaac
     7201 gcgacgttaa cagcgaaagg ttgcgaaaat ccgtcggtgt agaacgtacg atggcggaag
     7261 atattcatca ctggtctgaa tgtgaagcga tcatcgagcg gctttaccct gaactggagc
     7321 gtcgtttggc aaaggtaaaa cctgatttgc tgattgcccg ccagggggtg aaattaaagt
     7381 tcgacgattt tcagcaaacc acccaggaac acgtctggcc gcggctgaat aaatctgatc
     7441 taatcgccac cgcgcgtaaa acctgggatg aacgccgcgg cgggcgcggt gtgcgactgg
     7501 tggggctgca tgtgacgttg cttgacccgc aaatggaaag acaactggtg ctgggattat
     7561 gaataacata caaataagaa actatcagcc tggtgatttt cagcaactat gcgctatttt
     7621 ccttcgagct gttacgatga cggcgagtca gcactattct ccacagcaaa ttgccgcctg
     7681 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
     7741 agttattaat gcacaaccgg ttggttttat tacccgcatt ggacattata tcgatatgtt
     7801 atttgttgaa ccggaataca cccgacgcgg agttgccagc gctttgttaa aaccattgat
     7861 taagtctgaa tccgaactta cggtggacgc cagcataacc gcaaaaccct tttttgaacg
     7921 ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggaacgt ggtttactaa
     7981 tttttatatg cgatataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgt
     8041 ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct
     8101 ttatcaatga ctctttgcag acctttccag gattaattct tttttcttgc cctggattcg
     8161 tctgccattt cctgattttt attcataaat ggaatggtac atgggcaaat atattcgtcc
     8221 cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat
     8281 ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgttgtgg ggatgccaga
     8341 tttacatccc ggacgcggct acccgattgg cgcagcgttc ttctctgttg gtcgttttta
     8401 cccggcactg gtcggcaatg atatcggctg cggcatggcg ctatggcaaa cagatattct
     8461 cgctcgcaaa tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc
     8521 tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcacaa
     8581 ctcgcttggc tccatcggtg gcggtaacca cttcgcagaa ctgcaacaga ttgatcaaat
     8641 tatcgacgcg gaactgtttg cacgggctgg tctggatgcg cagcatctgc aactactggt
     8701 tcatagcggc tcgaggggat tggggcagtc tattttacag cggcatattg cctcgttttc
     8761 gcatcatggt ttgcctgaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga
     8821 tgcgctggcg tttgcgcgta ttaatcgcca gctgatcgct ttgcgcataa tgcaacaagt
     8881 taaggccacc ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgccaaat
     8941 cggcgatcaa cagggctggt tgcatcgtaa aggcgccacg ccggatgaca acggtctggt
     9001 gattattccc ggttcacgcg gcgattactc ctggctggtt aaacctgttg cgaacgaaaa
     9061 aacgctccac tcgctggcgc atggggcggg gcgtaaatgg gggcgcaccg agtgtaaagg
     9121 gcgtctggca gcgaaataca cggcgacgca actctcgcgg actgaacttg gcagtcgggt
     9181 aatttgtcgc gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga
     9241 aagcgtggtg caatgtctgg tgctggctgg gttaattatt cctgtcgcgc gactgcgccc
     9301 ggtgctgacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctct
     9361 gctcaggggc cggaagaatg ttgtctcgca gtgaaaaaag cactggacag gctgattaaa
     9421 gaagctaccc gacaggacgt cgcggtaacg gtgctggaaa cagaaacggg tcgctactct
     9481 gacacactgc gttcggcgct gatttctctg gatggcgata acgcatgggc tctaagcgaa
     9541 agctggtgcg gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc
     9601 aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca
     9661 atccgttatg agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac
     9721 tcggcggtac gcgccacgca tctggcatcc ggtattagcg tgaaggttca gtcagagcgc
     9781 agtcagcatg ctaacaaacg gctggcgcga ttactgattg cctggaagct ggaacaacag
     9841 caacaggaaa atagcgcggc gctgaaatcg cagcggcgaa tgttccatca ccagattgaa
     9901 cgtggcaacc cgcgacgaac gttcacaggg atggctttta tcgaaggata atgaaggatg
     9961 aaactgccgg aaggcgttaa acgccatccg gcagcaaatc aaataattac ttcgccggaa
    10021 tttctttcag cagttcagtc agcagtgtcc agtaatggcc aacgctttcg atatgaactt
    10081 gctcatccgg ggagtgtgga ccggtgatgg ttggcccgat agacaccatg tccatttccg
    10141 gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggcg
    10201 tcttgttgaa cagacgctga taggtttcac gtaccagatg cattaccgga gagttagcgt
    10261 ccggctgcca gccaggatat gcgccttttg cttcggtttt cgcacctgcc agtttaccca
    10321 gcgaatccag catgctcact acgtagtctt taccgctgtc gatcagagaa cggatcaggc
    10381 agtgaatttc tacgttattg tcggtcatgg tcaccacacc gacgttcagg gaggtttcaa
    10441 ccacgccttt ggctacatcg gaattacgga taactccgtt cggggtggcg ttcagcagac
    10501 gaataaaggt atcgcgagat gtcgccgtta atgcagcttt gtcgttcgct acggagtcca
    10561 gcagcaatgc cagattcttc tctttttctg ccagttcgtt tttcaggatc tcctgatagg
    10621 tatccaccag agatttcagg gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg
    10681 cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcaataaga cgcaggtcca
    10741 gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa
    10801 cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaaa ttgaaggttt
    10861 caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc
    10921 ccgcacatcc catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag
    10981 cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct tcagtcatgg
    11041 tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcgg
    11101 aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc
    11161 catcaatata aggctggata ggatctttag tgaagtcatg cacggtgtcg ttattttttt
    11221 gcggcaccat atcgaggtgg gcctgcaaga cgaccggttt acgattttcc atacctgcgg
    11281 tagcaggttt acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct
    11341 cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa
    11401 tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag
    11461 acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca
    11521 gaaaatgttg tcaacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
    11581 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
    11641 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
    11701 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
    11761 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
    11821 attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt
    11881 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    11941 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtagat
    12001 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    12061 ttcgcaaaac cggctggtcg tccgctggtt gatgattatg ttgttgatat cccgcaaaat
    12121 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    12181 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    12241 atagtttcca gtaagtattc tggaggctgc atctatgaca caggcaaacc tgagcgaaac
    12301 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttagtca
    12361 cggcgcacaa ctgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    12421 catgattaac cgtctgatgt ggatctggcg cggcattgac ccccgtgaaa tcctcgaagt
    12481 ccaggcgcgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    12541 gattggctac cgcggcggca actggattta tgagtgggct acccaggcga tggtgtggca
    12601 acaaaaagcc tgtgcggaag aagatccgca actcagcggt cgacactggc tgcatgcggc
    12661 tacgttgtac aacattgctg cctatcctca tctgaaagga gatgacctgg ccgagcaagc
    12721 gcaggctttg tcaaaccgcg cctatgaaga ggccgctcag cgtctgccgg gcacgatgcg
    12781 gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa
    12841 aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacaga
    12901 ctattacagc ctgtatgaac gttattttgc gctgcgcggc attgcgatgc tgactattga
    12961 tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca
    13021 tcagcacgtt ttaaaggcgt tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc
    13081 ctttggtttc cgtttcggcg ctaacgttgc cgtacgtctg gcataccttg aatcgccgcg
    13141 tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg
    13201 ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga
    13261 tgcttctgac gaagcgttgc gcgtggagct gaatcgctat tcattaaaag tgcaagggtt
    13321 gctgggacgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag
    13381 cccggaagag gactcacgct taatcacgtc gtcatctgct gacggtaaat tattagagat
    13441 cccatttaac ccggtgtatc ggaattttga caaaggcctt caggaaatca cagactggat
    13501 cgaaaaacgt ttgtgttaaa aatttgctaa attttaccaa tttggtaaaa cagttgcatc
    13561 acaacaggag attgcaatga cgttaccgag tggacacccg aagagcagat tagtcaaaaa
    13621 atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt
    13681 cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg
    13741 ctggtggatg gaactggaag cgcaggaatc ccgttttacc tacagttacc agtttggtct
    13801 gttcgataaa gcaggcgact ggacgagtgt tcagataaaa gatgccgaag tggttgaacg
    13861 actggagcac accctgcgtg aatttcacga gaagctgcgt gagctgctgg cgacgctgaa
    13921 tctgaagctg gaaccggcag atgattttcg tgatgaaccg gtgaagttaa cggcgtgaat
    13981 gaaatgtgcc ggatgcatca catccggcaa tatccattaa aactgatagg tcatgccaac
    14041 cgcgacaata tcatcattat taatattcaa tttgttatcg ctatccagtt ggttgatttt
    14101 ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccaa catcgacata
    14161 attgaccaga tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc
    14221 cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgcgt
    14281 cttattggca aagccgccag agattggcgt cattttacgt gtttcagaat agaaagttgc
    14341 cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc
    14401 tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatca gagttggtat aggctccact
    14461 gatggcgaaa tcgctgccac caaagtcata tgtcaatgac gtgccgaagc catcgccgtt
    14521 ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc
    14581 atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt
    14641 cataaagttg tcggtctgcg ccgaggagtc gccaccaaat tccgggaaca tatcggtcca
    14701 ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt
    14761 atatttcaac ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt
    14821 attaccggca aactcagctt cccaacgacc ataaccagtc agttgatcgt taatttgtgt
    14881 ttcgccttta aaaccaaaac ggatataact ctggtcgcca tctttactgt cattatcact
    14941 catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt ctttgttata
    15001 tatttctgcg gcctgtacgg atacagatgc cacaatgccc atcaccacta atgccagagt
    15061 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagtcc
    15121 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaataat
    15181 attcttaaaa tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg
    15241 cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga
    15301 cagtcctgct aaaacgttcg tttgatatca ttttttccta aaattgaatg gcagagaatc
    15361 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
    15421 cgccgcctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc
    15481 gccgggcatc ggattgttat tgtgacgtcg ggtgcgattg ccgccggacg tgagcacctg
    15541 gggtacccgg aattgccagc gactatcgct tcaaaacagc tgctggcggc ggtagggcag
    15601 agtcgactga ttcaactgtg ggaacagctg ttttcaattt acggcattca tgtcgggcag
    15661 atgttgctga cccgtgctga tatggaagat cgtgaacgct tcctgaacgc ccgcgatacc
    15721 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatta atgagaacga tgctgtcgct
    15781 acggcagaga ttaaggtcgg tgataacgat aacctttctg cgctggcggc gattctggcg
    15841 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc
    15901 agcaatccgc aggcagaact gattaaagat gtgtacggca ttgatgacgc actgcgcgcg
    15961 attgccggtg atagcgtttc aggcctcgga actggcggta tgagcaccaa attgcaggcc
    16021 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
    16081 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
    16141 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc ctgcgggtga aatcacggta
    16201 gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc gaaaggcatt
    16261 aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gtatttgcaa cctcgaaggt
    16321 cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccgga
    16381 caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac
    16441 cgtgatgaca tgattacccg ttaaggagct ggccgatgct ggaacaaatg ggcattgccg
    16501 cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgtgtgctgg
    16561 aaaaaatcgc cgatgaactg gaagcacaaa gcgaaagtat cctcaacgct aacgcgcagg
    16621 atgttgcaga cgcgcgtgcc aatggcctta gcgaagcgat gcttgaccgt ctgacactga
    16681 cgcccgcacg gctgaaaggc attgccgatg atgtgcgcca ggtgtgcaac ctcgccgatc
    16741 cggtggggca ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt gagcgtcgtc
    16801 gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg
    16861 tcgcttcgct gtgcctgaaa acaggtaacg cagtgatcct gcgcggcggc aaagaaacgt
    16921 gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaactct tgtggcttac
    16981 cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc
    17041 gtatggataa atacatcgac atgctgatcc cgcgcggcgg ggctggtttg cataaactgt
    17101 gccgcgagca gtcgacaatc ccggtgatca caggcggtat aggcgtatgc catatttacg
    17161 ttgatgaaag cgcagagatc gctgaagccc tgaaagtaat tgtcaacgcg aaaactcagc
    17221 gtccgagcac gtgtaatacg gtagaaacgt tgctggtgaa taaaaacatc gctgatagct
    17281 tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacgttacac gctgatacag
    17341 ccgcgctggc gcagttgcag gcaggccccg cgaaggtggt ggctgttaaa gccgaagagt
    17401 atgacgatga gtttctgtca ttagatttga acgtcaaaat cgttagcgat ctggatgacg
    17461 ccattgccca tattcgtgaa cacggcacgc aacactccga tgcgatcctg acccgcgata
    17521 tgcgcaacgc ccagcgtttt gttaacgaag tggattcctc cgctgtttac gttaacgcct
    17581 ctacgcgttt taccgacggc ggccagtttg gactgggggc ggaagtggcg gtaagcacac
    17641 aaaaactcca cgcgcgcggc ccaatggggc tggaagcact gaccacttac aagtggatcg
    17701 gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt
    17761 gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac
    17821 gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta
    17881 atgcgaaggt cgtaggttcg actcctatta tcgg
//
LOCUS       DACADA010000490         5810 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.231, whole genome shotgun sequence.
ACCESSION   DACADA010000490 DACADA010000000
VERSION     DACADA010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.231"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(35..784)
                     /gene="intI1"
                     /locus_tag="ISB17_005087"
                     /pseudo
     CDS             complement(35..784)
                     /gene="intI1"
                     /locus_tag="ISB17_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            942..1415
                     /locus_tag="ISB17_005088"
     CDS             942..1415
                     /locus_tag="ISB17_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HAP1982889.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1546..2334
                     /locus_tag="ISB17_005089"
     CDS             1546..2334
                     /locus_tag="ISB17_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HAP1982890.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2540..2887
                     /locus_tag="ISB17_005090"
     CDS             2540..2887
                     /locus_tag="ISB17_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HAP1982891.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2881..3720
                     /gene="sul1"
                     /locus_tag="ISB17_005091"
     CDS             2881..3720
                     /gene="sul1"
                     /locus_tag="ISB17_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HAP1982892.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3848..4051
                     /locus_tag="ISB17_005092"
     CDS             3848..4051
                     /locus_tag="ISB17_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982893.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4207..5412
                     /gene="chrA"
                     /locus_tag="ISB17_005093"
     CDS             4207..5412
                     /gene="chrA"
                     /locus_tag="ISB17_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HAP1982894.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5423..5728
                     /locus_tag="ISB17_005094"
     CDS             5423..5728
                     /locus_tag="ISB17_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1982895.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
BASE COUNT         1213 a         1584 c         1639 g         1374 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccg acgcacgaca ccgctccgtg
       61 gatcggtcga atgcgtgtgc tgcgcaaaaa cccagaacca cagccaggaa tgcccggcgc
      121 gcggatactt ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt
      181 ccttcagcca ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct
      241 cgggtaacat caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt
      301 gatcgaaatc cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc
      361 catacagaag ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca
      421 cttcatccgg ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct
      481 gaagccaggg cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg
      541 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct
      601 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
      661 gaacccagtg gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
      721 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      781 tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag
      841 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      901 gcagtcgccc taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt
      961 ctgcagtgtc agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag
     1021 gtgagcaact actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa
     1081 catttgactc tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa
     1141 tttcaagctc aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc
     1201 tatcaaaagt tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta
     1261 ttgaaaaagc agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca
     1321 aattccctat aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata
     1381 taaattatac ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt
     1441 cgctccttgg acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac
     1501 aaagccgcaa cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc
     1561 cctgcacaag ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct
     1621 gcgacactgg acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac
     1681 agcgacatag acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg
     1741 ctaatgctcg atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg
     1801 ctggagctaa ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt
     1861 gagcttcagt tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc
     1921 gttctggatc acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt
     1981 ctaggcccat ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg
     2041 cttttcgaca ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac
     2101 gtcgttcttg ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag
     2161 gacgttgctg ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc
     2221 aaggcacgcg cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag
     2281 acggccgcgt tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca
     2341 tgtgcgaaaa gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc
     2401 ggacgggctg cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac
     2461 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     2521 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga
     2581 agtaatcgca acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc
     2641 cgttgtcata atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat
     2701 ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat
     2761 tgcctggttg cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat
     2821 aattgctgcc tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc
     2881 atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg
     2941 cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac
     3001 gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat
     3061 gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca
     3121 atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg
     3181 aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc
     3241 aggctggtgg ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt
     3301 cgacccgaag acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg
     3361 cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg
     3421 agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg
     3481 ttggggcttc cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc
     3541 cttcctgtaa aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat
     3601 ggcgctgact acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg
     3661 gaaaccctcg cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag
     3721 cattcacctt ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag
     3781 acatgctggg ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg
     3841 ggagcgaatg gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga
     3901 cgaggttgtg cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc
     3961 ctgggacgtc gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct
     4021 cctagggatc gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc
     4081 ctgtgaaagc cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat
     4141 atcgaatttc gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc
     4201 gaaagtatga cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt
     4261 ctgcggcttg gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc
     4321 gaatttgtca cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt
     4381 tgtcagttct tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg
     4441 gctggataca gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc
     4501 ttgatccttt ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg
     4561 catggcttaa aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt
     4621 aacctatgca cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta
     4681 cttgtgccgt ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg
     4741 ttattgttca agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac
     4801 cgggccggcg tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg
     4861 gccgaactga tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca
     4921 ctggtgttcg gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc
     4981 ggctgggtca acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc
     5041 cctttgttca cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg
     5101 atcggcggca ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga
     5161 tcaatgccat tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg
     5221 atcaatgccg ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc
     5281 gccatctttc agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc
     5341 tggaagctcc cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc
     5401 gtcgctctgt gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct
     5461 gcacatcctt caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct
     5521 acgccgacac ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga
     5581 aaaaaaaggc tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta
     5641 tgacatcact gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct
     5701 gttcggagag ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc
     5761 ctaactggat tgtcattttc agaagacgac tgcaccagtt gattgggcgt
//
LOCUS       DACADA010000491         4523 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.252, whole genome shotgun sequence.
ACCESSION   DACADA010000491 DACADA010000000
VERSION     DACADA010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.252"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            49..909
                     /locus_tag="ISB17_005095"
     CDS             49..909
                     /locus_tag="ISB17_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP1982896.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1142..1450)
                     /locus_tag="ISB17_005096"
     CDS             complement(1142..1450)
                     /locus_tag="ISB17_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982897.1"
                     /translation="MIIAAEVKRDEQGYWTHPALVRSGCETSAELSYWLRTHKLQCFV
                     MTMRDEAPEAFAAGFTDEAPDARGWIPEPPDDDGWFLGSVHDTGDGPVCYWFRHTTKS
                     "
     gene            complement(1447..2097)
                     /locus_tag="ISB17_005097"
     CDS             complement(1447..2097)
                     /locus_tag="ISB17_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAP1982898.1"
                     /translation="MAKILMMAGSTVVVLSLLGFLVLLLKGRAVTKTSPVLRSLKALG
                     IRPSEAEQRLCRQRVWVGNDTLMTPREQHFFRALLRHTSRTRWLLCPQVRVADIATLS
                     PHIRPRSRTWWQLFRMASQWHCDVVIVDIHTFAIIGAVELDDASHQKKHRIRRDILLE
                     EVLRQAGIPLLRDRDSESLVRKVVEFLKYREAGAEEKSITVMKSTTKHSEHKEKEK"
     gene            complement(2153..2797)
                     /locus_tag="ISB17_005098"
     CDS             complement(2153..2797)
                     /locus_tag="ISB17_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982899.1"
                     /translation="MFRYFILRPEQQLFCYLYGCALALVQMVLFSPVSRASGFYLVAL
                     SVALFWAGLALYTRHIDRMRKPEVSPLVSIRDGIQVVAEVPRYEKARLEWEILRDDEV
                     FRQQRCELTGLTGRVISRGLLYTPAVMLVGIGILAWGSPQDAIRLINALRNMPAAELV
                     HQIGFVLCHFLQISVISVLIADVVAGRGLPNVFRRALLDRLPAEFCLIRRGTER"
     gene            complement(2847..3449)
                     /locus_tag="ISB17_005099"
     CDS             complement(2847..3449)
                     /locus_tag="ISB17_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982900.1"
                     /translation="MTMTEKIIALVDYENIGTLENVRLSRYERLILFTGPQQEFIRFP
                     AVTYAGDISVSVFQVSNVSKNNVDFHLVFELGRLSATAPEETIFHIISNDKGYDGVIA
                     ELCRAGRRCCRISSPCSAEKPKAAPMVISNDEVLHLTDKVQSLSKKSAGNRPANTSSL
                     MNYIKSLSGNTKGMALYGRVKEELIRRGVIAVYEKTVVWR"
     gene            complement(3610..4203)
                     /locus_tag="ISB17_005100"
     CDS             complement(3610..4203)
                     /locus_tag="ISB17_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAP1982901.1"
                     /translation="MAEEKRPVLSLKRKPAENSTAPAEATPAPGVVRRKKVVVVSSPP
                     AWKAKKAKLEKAKQAAEADARNAAPAPDKAVKTPPPVRYLRLLPPEQAIMTLKAFWPQ
                     LFDGNSPRLLARGMREQLFADIVNRDLPLSHKQVIKCLKSLTRSADYLSRMKVGASRY
                     DLQGNAVATVTAEEAQYASERMMKELLRAERKMSQTL"
     gene            complement(4275..>4523)
                     /locus_tag="ISB17_005101"
     CDS             complement(4275..>4523)
                     /locus_tag="ISB17_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982902.1"
                     /translation="HRCYSPISFTVIRMGYGVLWALMVLLMNMHDVSESVAGLAIALL
                     TLMVCSSAGERVKRFWPRQFFVMFYAVHLAVLGIVVSM"
BASE COUNT         1113 a         1222 c         1074 g         1114 t
ORIGIN      
        1 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
       61 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      241 ccaatgatga gcacttttaa agttctgcta tgtggtgcgg tattatcccg tgttgacgcc
      301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
      601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      901 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa cgggtgttta agaaacgccg
     1081 ggagcgggga aactggcttt tccccgttcc cggggttgga caacgacgcg atgcggaggc
     1141 gtcaggattt tgtggtatga cgaaaccagt agcagacggg tccgtcgccg gtatcgtgga
     1201 cagatcccag aaaccagcca tcatcatccg gtggctccgg tatccagcca cgggcatccg
     1261 gggcttcatc cgtaaatccg gcagcgaaag cttcaggcgc ttcatcgcgc attgtcatca
     1321 caaagcactg gagtttatgg gttctgagcc agtaacttaa ttctgcggaa gtttcacagc
     1381 cacttcgtac cagcgcagga tgtgtccagt acccctgttc atcacgtttc acttcagcgg
     1441 cgataatcat ttttctttct ccttgtgttc gctatgcttt gtcgttgatt tcataacggt
     1501 tatggatttt tcttcagcac cggcttcccg gtatttcaga aactctacga cctttcttac
     1561 cagactctcc gaatcccgat cgcgtagtag tggaatgccg gcctgcctca gaacctcttc
     1621 cagcagaata tcgcgccgga tacggtgttt tttctggtgt gaggcatcat ccagttcaac
     1681 tgcgccgatg atagcgaagg tatggatgtc aacgatgacc acatcgcagt gccactgtga
     1741 tgccatccgg aagagttgcc accaggtccg ggatcgtggc cggatatggg gagagagtgt
     1801 ggcaatatct gccacccgga cctgaggaca caacagccag cgtgttcggc tggtatgcct
     1861 cagcaatgcc cggaaaaaat gttgttcccg tggcgtcatc agtgtgtcat tacccaccca
     1921 gacccgctgg cgacacaacc gttgctcagc ctctgagggg cgaataccca gggctttcag
     1981 tgacctcaga accggggaag tcttcgttac tgcgcgtcct ttcagaagca gcaccagaaa
     2041 tcccagcaaa ctcagcacaa caacggtact gcccgccatc attaatatct ttgccatgta
     2101 ttttattccc tgatgttcct ttctttcccg atgcgttttc ctgtcgtctt cctcatcttt
     2161 ctgtccctct tctgataagg cagaactctg ccggcagcct gtcgagcaac gcgcggcgaa
     2221 atacattcgg cagtcctctc ccggctacaa catcagcaat cagaactgat atgacgctaa
     2281 tctgcaggaa gtggcacagg acaaaaccaa tctgatgcac cagttccgca gcaggcatat
     2341 tacgaagagc attaatcagc cggatggcat cctgtgggga accccaggcg agaataccga
     2401 taccgaccag catcaccgcc ggcgtataca acagcccccg gctgataacc cgtccggtca
     2461 ggcctgtaag ctcacacctt tgctggcgaa acacctcatc atcccggagg atttcccatt
     2521 ccagccgggc cttttcatag cgcggcacct cggccaccac ctgaataccg tcgcgaatac
     2581 tgaccagcgg tgagacctct ggctttctca tccggtcgat gtgccgggtg tacagggcca
     2641 gaccggccca gaacagcgcg acagataacg caaccagata aaatccgctt gcccgggata
     2701 caggagaaaa taaaaccatc tgaaccagtg ccagcgcgca gccgtataaa taacagaata
     2761 actgctgctc agggcgcaga atgaaatatc tgaacatatt gattcctttt tagataaaaa
     2821 taacattcac agccgtctta atttcattat cgccagacca ctgttttctc atacaccgca
     2881 atcacaccac gcctgattaa ttcctcttta acgcgaccat aaagcgccat gccttttgta
     2941 ttaccggaca gggatttaat ataattcatc agggatgaag tattcgccgg gcggttccct
     3001 gcagattttt tacttaatga ctggacttta tcggtcaggt gcagcacttc atcgttactg
     3061 attaccatag gtgccgcctt cggtttctct gcagagcaag gcgatgatat acggcaacat
     3121 cgtctccccg ccctgcatag ctcggcaatg acgccatcat agcctttatc attcgagata
     3181 atatgaaata tcgtctcttc cggtgcagtc gcgctaagtc ttcccagctc aaacaccaga
     3241 tggaagtcca cattattttt tgacacattc gaaacctgga atacgcttac cgatatatca
     3301 cctgcatacg ttacagctgg aaaacgtata aactcctgct gaggcccggt aaataaaata
     3361 aggcgttcat aacgggacag acggacattt tcaagtgtac caatattttc ataatctacg
     3421 agagcaataa ttttttcagt catcgtcatc cagaatctgt ctgcacggac agttatgatg
     3481 ccgcagcctg taatgcggca aatataaaat gaatacctgt tgctatttca ggcataccct
     3541 cagctaattt tcgttacatc tattagctga ggattcccag gtataattat caggaatggg
     3601 taaatataat taaagggtct gactcatctt tcgttcagct cgcagcagct ccttcatcat
     3661 gcgctcactg gcgtactgcg cttcctctgc agtgacggtc gcaacggcat tcccctgcag
     3721 atcatagcgg gaggccccca ccttcatccg ggacagatag tccgcagacc gggtcagact
     3781 cttcaggcac ttaatgacct gtttatgcga cagcggcagg tcgcggttca caatgtcggc
     3841 aaacagctgc tcgcgcattc ccctcgccag cagacggggt gagtttccgt cgaacagctg
     3901 tggccagaat gccttcaggg tcatgatggc ctgttccggc ggcagcaggc gaagataacg
     3961 caccggaggt ggcgtcttta ccgctttatc cggcgccggc gcagcgttgc gcgcgtcggc
     4021 ttcggccgct tgtttagcct tctcaagttt cgctttcttt gccttccagg ccggcgggga
     4081 cgagaccacc acgaccttct ttcggcgcac cacaccagga gcaggggttg cctctgcggg
     4141 ggccgtgctg ttctccgccg gttttcgttt caggctcagt acagggcgtt tttcttctgc
     4201 cataacaatt gcctatcgtt cagttaataa gagctactat acctgaacgg tatcaccctt
     4261 tcctgctcct gcgttcacat tgagacgaca atacccagta ccgccaggtg cacggcatag
     4321 aacatcacga agaactgccg cggccagaag cgtttcacac gttctccggc agatgagcac
     4381 accatcagcg tcagcagcgc aatcgccagt ccggccactg attcactcac gtcatgcata
     4441 ttcatcagca acaccatgag ggcccacaac accccgtatc ccatgcgtat taccgtgaag
     4501 gagatcggtg agtaacatcg atg
//
LOCUS       DACADA010000492         4190 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.264, whole genome shotgun sequence.
ACCESSION   DACADA010000492 DACADA010000000
VERSION     DACADA010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.264"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            108..1013
                     /gene="mph(A)"
                     /locus_tag="ISB17_005102"
     CDS             108..1013
                     /gene="mph(A)"
                     /locus_tag="ISB17_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP1982903.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1010..2248
                     /locus_tag="ISB17_005103"
     CDS             1010..2248
                     /locus_tag="ISB17_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1982904.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2248..2832
                     /locus_tag="ISB17_005104"
     CDS             2248..2832
                     /locus_tag="ISB17_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1982905.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3325..4089)
                     /locus_tag="ISB17_005105"
     CDS             complement(3325..4089)
                     /locus_tag="ISB17_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP1982906.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          712 a         1306 c         1305 g          867 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccgg attgaatata accgacgtga
       61 ctgttacatt taggtggcta aacccgtcaa gccctcagga gtgaatcatg accgtagtca
      121 cgaccgccga tacctcccaa ctgtacgcac ttgcagcccg acatgggctc aagctccatg
      181 gcccgctgac tgtcaatgag cttgggctcg actataggat cgtgatcgcc accgtcgacg
      241 atggacgtcg gtgggtgctg cgcatcccgc gccgagccga ggtaagcgcg aaggtcgaac
      301 cagaggcgcg ggtgctggca atgctcaaga atcgcctgcc gttcgcggtg ccggactggc
      361 gcgtggccaa cgccgagctc gttgcctatc ccatgctcga agactcgact gcgatggtca
      421 tccagcctgg ttcgtccacg cccgactggg tcgtgccgca ggactcggag gtcttcgcgg
      481 agagcttcgc gaccgcgctc gccgccctgc atgccgtccc catttccgcc gccgtggatg
      541 cggggatgct catccgtaca ccgacgcagg cccgtcagaa ggtggccgac gacgttgacc
      601 gcgtccgacg cgagttcgtg gtgaacgaca agcgcctcca ccggtggcag cgctggctcg
      661 acgacgattc gtcgtggcca gatttctccg tggtggtgca tggcgatctc tacgtgggcc
      721 atgtgctcat cgacaacacg gagcgcgtca gcgggatgat cgactggagc gaggcccgcg
      781 ttgatgaccc tgccatcgac atggccgcgc accttatggt ctttggtgaa gaggggctcg
      841 cgaagctcct cctcacgtat gaagcggccg gtggccgggt gtggccgcgg ctcgcccacc
      901 acatcgcgga gcgccttgcg ttcggggcgg tcacctacgc actcttcgcc ctcgactcgg
      961 gtaacgaaga gtacctcgct gcggcgaagg cgcagctcgc cgcagcggaa tgagcgaacg
     1021 tcgatatagc ccgctcgcga cgctgttcgc ggcgaccttt ctcttccgga tcggcaacgc
     1081 ggtggcggcc ctcgcgcttc catggttcgt cctgtctcat acaaagagcg cggcctgggc
     1141 gggcgccacg gccgctagca gcgtcatcgc gaccatcatc ggcgcgtggg ttggtggtgg
     1201 cctcgtcgat cggttcgggc gcgcgcccgt cgcattgatc tcgggtgtgg tgggcggcgt
     1261 ggccatggcg agcatcccac tgctcgatgc cgttggcgcc ctctcgaaca ctgggctgat
     1321 cgcttgcgtg gtgctcggtg ccgcgttcga cgcacccggt atggccgcgc aggacagtga
     1381 gctgcccaaa ctcggccacg tcgccgggct ctccgttgag cgcgtctcgt cactgaaagc
     1441 ggtgatcggg aacgtcgcga ttctaggtgg cccggccctt gggggggccg caatcggcct
     1501 gcttggcgct gcgccaacgc tcgggctgac ggcgttctgc tccgtccttg caggtctgct
     1561 cggcgcgtgg gtgcttcccg cgcgtgccgc tcggacgatg accacgacgg cgactctctc
     1621 catgcgcgcc ggcgtcgctt ttctctggag cgaacccctg ctgcgccctc tctttggtat
     1681 agtgatgatc ttcgtgggca tcgttggcgc caacggcagc gtcatcatgc ctgcgctgtt
     1741 tgtagatgca ggacgccaag tagcagagct cgggctgttc tcctcaatga tgggggctgg
     1801 tggtctcctt ggcattgcca ttcatgcgtc ggtcggcgcc cggatatcag cgcagaactg
     1861 gctggcggtg gcattttgtg gctctgcggt gggctcgctt ctgctttcac agttgccagg
     1921 cgtgccggtg ctgatgttgt tgggcgcgct cgtgggactg ctgaccggct cagtctctcc
     1981 cattctcaac gctgccatct acaaccgcac gccgccagaa cttctcggcc gggtactcgg
     2041 cacggtctcg gcggtgatgc tgtcagcctc gcccatggtt atgcttgcgg ccggcgcgtt
     2101 tgtcgacctt gctggtccgc tccctggcct cgttgtatcg gccgtgtttg cggggctcgt
     2161 ggctctactc tcgctccgtc ttcaatttgc tacaatggcg gcggcagcca cagcctccgc
     2221 cccaacccat acagaaggtg aacactgatg ccccgcccca agctcaagtc cgatgacgag
     2281 gtactcgagg ccgccaccgt agtgctgaag cgttgcggtc ccatagagtt cacgctcagc
     2341 ggagtagcaa aggaggtggg gctctcccgc gcagcgttaa tccagcgctt caccaaccgc
     2401 gatacgctgc tggtgaggat gatggagcgc ggcgtcgagc aggtgcggca ttacctgaat
     2461 gcgataccga taggcgcagg gccgcaaggg ctctgggaat ttttgcaggt gctcgttcgg
     2521 agcatgaaca ctcgcaacga cttctcggtg aactatctca tctcctggta cgagctccag
     2581 gtgccggagc tacgcacgct tgcgatccag cggaaccgcg cggtggtgga ggggatccgc
     2641 aagcgactgc ccccaggtgc tcctgcggca gctgagttgc tcctgcactc ggtcatcgct
     2701 ggcgcgacga tgcagtgggc cgtcgatccg gatggtgagc tagctgatca tgtgctggct
     2761 cagatcgctg ccatcctgtg tttaatgttt cccgaacacg acgatttcca actcctccag
     2821 gcacatgcgt aaacggaggt gtgcagagtc cctgcggcag gcgacgaaca cgaccgtcgt
     2881 cgattagtac cggtacggtc ggtggtatcg aagtcttgat caccactcag gtctacggct
     2941 tacaaatggt gaccatcccg atacttgcgt cagagcaccg ggccgattct ttgacagtga
     3001 atcactcccg taaggttgtg ccggtgtggg tgtcccgggt cgagacgata ctccgccaat
     3061 gcgcccagca aacaacctgg ccatcgcagg tggtggggag cggtgtggcg gatgagttgg
     3121 acaagttggt gtagcagcac gagcacggcg agataacatc gcaggagttc gacatgctca
     3181 agagacagct gattgcgaat cgcgatgcag attcataacc cgattgcggg ttggcttcac
     3241 tccaccatca ccgagcagac tagcacggcg ggctctgttg caaagattgg cggcagtcag
     3301 aggtaggctg tcgctctgcg ccgatcaggc ggctgctgcg aaatggtggt tgagcatgcc
     3361 catggcctcc gtcagcgccg agggcccaat gccaaaagct ctctccacaa ggcgcacctc
     3421 gcccctgatg ccgggctgca ggcaccaggg gcgagcctgt cctttgcgca gggctcgcat
     3481 gacttcgaat cccttgatcg tggcataggc cgtggggatc gatttgaaac cgcgcaccgg
     3541 cttgatcagt atcttgagct ttccgtgatc ggcctcgatc acgttattga gatacttcac
     3601 ctgccggtgg gccgtctccc ggtccagctt tccttcgcgc ttcaattcgg tgatcgctgc
     3661 accatagctc ggcgctttgt cggtattgag cgtggcaggc ttttcccagt gcttcaggcc
     3721 tcgcagggcc ttgcccagga accgcttcgc tgccttggcg ctgcgggtcg gcgacaggta
     3781 gaaatcgatc gtgtcgcccc gcttgtcgac tgcccggtac aggtaggtcc acttgccccg
     3841 caccttgacg taggtttcat ccaggcgcca gctcggatca aagccacgcc gccagaacca
     3901 gcgcagccgc ttctccatct ccggggcgta gcactggacc cagcgataga tcgtcgtatg
     3961 gtcgaccgaa atgccgcgtt ccgccagcat ttcctcaagg tcgcgatagc tgatcggata
     4021 gcgacaatac cagcgcaccg cccacaggat cacatcaccc tggaaatggc gccacttgaa
     4081 atccgtcatc gttccgtccg tccaatctcc gccaagcatg ctcaagcttc acgatttttg
     4141 caacagagcc cacacgagta ttgagcatag tcgagattgg tgcagatcac
//
LOCUS       DACADA010000493         3360 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.282, whole genome shotgun sequence.
ACCESSION   DACADA010000493 DACADA010000000
VERSION     DACADA010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.282"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            complement(<1..142)
                     /locus_tag="ISB17_005106"
     CDS             complement(<1..142)
                     /locus_tag="ISB17_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982907.1"
                     /translation="MKVELGVAAGCNQAATAGIRGLTLSGTKTHVKKSFFNIIEAFIR
                     VIV"
     gene            159..1040
                     /locus_tag="ISB17_005107"
     CDS             159..1040
                     /locus_tag="ISB17_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="HAP1982908.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSI
                     GVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALV
                     PWSPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFR
                     LDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNH
                     RIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAV
                     IAAAARLALEGLGVNGQ"
     gene            complement(1290..2171)
                     /locus_tag="ISB17_005108"
                     /pseudo
     CDS             complement(1290..2171)
                     /locus_tag="ISB17_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
     gene            complement(2275..2571)
                     /locus_tag="ISB17_005109"
     CDS             complement(2275..2571)
                     /locus_tag="ISB17_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor protein KorC"
                     /protein_id="HAP1982909.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDV
                     ADLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
     gene            complement(2617..2772)
                     /locus_tag="ISB17_005110"
     CDS             complement(2617..2772)
                     /locus_tag="ISB17_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1982910.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYRE
                     AAATVTH"
     gene            complement(2900..3325)
                     /locus_tag="ISB17_005111"
     CDS             complement(2900..3325)
                     /locus_tag="ISB17_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1982911.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKG
                     LCREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYS
                     HLSFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
BASE COUNT          659 a         1044 c          911 g          746 t
ORIGIN      
        1 agacaataac cctgataaat gcttcaataa tattgaaaaa tgacttctta acgtgagttt
       61 tcgttccact gagcgtcaga cccctaatcc cagctgtagc ggcctgatta catccggccg
      121 ctacacctag ctccaccttc aaacaaggaa tatcgttgat gtcactgtat cgccgtctag
      181 ttctgctgtc ttgtctctca tggccgctgg ctggcttttc tgccaccgcg ctgaccaacc
      241 tcgtcgcgga accattcgct aaactcgaac aggactttgg cggctccatc ggtgtgtacg
      301 cgatggatac cggctcaggc gcaactgtaa gttaccgcgc tgaggagcgc ttcccactgt
      361 gcagctcatt caagggcttt cttgctgccg ctgtgctggc tcgcagccag cagcaggccg
      421 gcttgctgga cacacccatc cgttacggca aaaatgcgct ggttccgtgg tcacccatct
      481 cggaaaaata tctgacaaca ggcatgacgg tggcggagct gtccgcggcc gccgtgcaat
      541 acagtgataa cgccgccgcc aatttgttgc tgaaggagtt gggcggcccg gccgggctga
      601 cggccttcat gcgctctatc ggcgatacca cgttccgtct ggaccgctgg gagctggagc
      661 tgaactccgc catcccaggc gatgcgcgcg atacctcatc gccgcgcgcc gtgacggaaa
      721 gcttacaaaa actgacactg ggctctgcac tggctgcgcc gcagcggcag cagtttgttg
      781 attggctaaa gggaaacacg accggcaacc accgcatccg cgcggcggtg ccggcagact
      841 gggcagtcgg agacaaaacc ggaacctgcg gagtgtatgg cacggcaaat gactatgccg
      901 tcgtctggcc cactgggcgc gcacctattg tgttggccgt ctacacccgg gcgcctaaca
      961 aggatgacaa gcacagcgag gccgtcatcg ccgctgcggc tagactcgcg ctcgagggat
     1021 tgggcgtcaa cgggcagtaa ggctctgaaa atcatctatt ggcccaccac cgccgccctt
     1081 gcgggcggca tggattacca accactgtca catttaggct aggagtctgc gcggcagagc
     1141 cgtgtgaccg gttttctgta gagcactgac gatggcggcg gcgctctctg caattggcaa
     1201 ggcgtcggcg ccaaggatac caatcttgcg gcgcgcggcg tgttatgacg actggggtgc
     1261 atttgagccg ccccatttaa ccttcgccct cacagatacg ccattcgcct cagatttagc
     1321 gccatgcaga cgagcttcca ctcggcttgc accttgtcca ggcccctcat gctgaactga
     1381 cgcaatccca tcaccgcctt gatccaacca ttcggagcct ccacgatcga cttgcgccgg
     1441 cggtaagctg catcgccttg ctccgttttc aatttcgccg caatcgccgc cgtatgcgga
     1501 tgggtcttgg cattgacctt ggcatcttca cgtccctcgc ggccgagggc aacgatgaca
     1561 tcgccgtggt gatcggcgac ctttgccaga acagcctcac tacggaatcc cgcatccgcc
     1621 agcgtctggg ccggcatttc tccggtgttg gcctgaactg ctgccagcat gcccagcagc
     1681 gcctgactgt ccgcggcgca gttggtcaac tccgccgcca cgatgatctg gtgctcggca
     1741 tcgaccgctg tgtacccgtt gtagctctgc tcggagccac caccggcgtg tttcatgatc
     1801 cggctgtccg gatcggtgaa gctttcctga tcacggtcat ccggcacacc aaactcgcgt
     1861 ttgtacgagc caccgccctt gtccgagcca tccggatggc gaggccggcg gccatcgtct
     1921 tcgctgcgcc cccgggcctg gtccgcttca cgctggcgcg cttccaggcg cgcctttgcc
     1981 gcctggatcg cctccaggcg cttctcgcgg cgagaaatct cggcaggaat gtccagctcc
     2041 ggctcgttac gctcctggtc gtcggtagcc ttggcgcgat caagcagcgc cttgatctcg
     2101 caatgcaatt cgtcctcggc cggcttcatg cgcttatagc tcatcgcctt gtggtcgcca
     2161 tgtttggcgg cttcgtattc aggttcttta gagagatcct ttagatcgcc gttcctcact
     2221 actgccggga agtctcccca gggagcacgt tcggccttat gttcggtcat ggtttcaatc
     2281 ccggtcagcg tcccatatag ccccgaaacc tgccaggtgg gccaagatag accaggcacc
     2341 ataagcaata gggctagggt tggttttgct cacccaacgc ctgaaggttc gggttccctt
     2401 tccgctgcct ctctcaccag gcaaaccaac gagatcagcg acatcaacgc cactgagagg
     2461 cttactaagc ccttttcgtt gcctaatcag ctccaacacc tctttgattt catcggcggt
     2521 tggagcctgc caatcttcag cgaacggcct aagcgtttca ggtctaatca tgcgataaat
     2581 cctcaaatag ccccggtcaa tgccggggct tttttatcaa tgggtaacgg tcgcggcggc
     2641 ctcccgatac gccttcagtt caatcaagag caagccaagc tcccgcatct tcatgcgcgg
     2701 catatgaacg gggcggcggg ccgtcagagc gtcgccaatg tgttttgcaa gtgatacaat
     2761 atccgtattc atcgcgttta tcctcaaatt gaccggtgaa agcccctgtg attggttgca
     2821 gccataccag gggcaccatt tctttttcta cctatgcacc tcctttaaga gccccggcca
     2881 tcaccggggc tttctgttgc tagtctattg cggccaagat ggcctctttt tcgtcatgct
     2941 cacaagccca atcccgcaag ctgtgataca gccttgaaaa ccgctcctgg tcagcgcctt
     3001 ctgtgtgaaa tgacagatgg ctatagaggt agaggcaagc ggtaatcccg gcagcatcgg
     3061 cgctcatttg gccactaaag tagttcatgg cctcgatgtt cagcatttca tcacgacgcc
     3121 caggagccat ataaaagccg ccgttcgaca gctcccacat aagccaataa gccccgcgat
     3181 actcgcggca gagccctttc atttgctggt aaagcatcga ctcgaaaacc acatagaaac
     3241 ggcctacata gcggggcatt gcagccatac ggcgaggggt ggcgaccagg gagcagatca
     3301 ggggattaag ttctgtttgc atcatcggag ttctccggta ttgaccgtgg cgggtgcccg
//
LOCUS       DACADA010000494         3225 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.285, whole genome shotgun sequence.
ACCESSION   DACADA010000494 DACADA010000000
VERSION     DACADA010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.285"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            86..322
                     /locus_tag="ISB17_005112"
     CDS             86..322
                     /locus_tag="ISB17_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP1982912.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(354..980)
                     /gene="tetR(A)"
                     /locus_tag="ISB17_005113"
     CDS             complement(354..980)
                     /gene="tetR(A)"
                     /locus_tag="ISB17_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP1982913.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1086..2285
                     /gene="tet(A)"
                     /locus_tag="ISB17_005114"
     CDS             1086..2285
                     /gene="tet(A)"
                     /locus_tag="ISB17_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP1982914.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2317..3177)
                     /locus_tag="ISB17_005115"
     CDS             complement(2317..3177)
                     /locus_tag="ISB17_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP1982915.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          620 a         1011 c          984 g          610 t
ORIGIN      
        1 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
       61 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
      121 cttcccgcaa acgaccccga cccgcgccac ggcccacgag cggacgccgc agcaggagcg
      181 ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat
      241 gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt
      301 tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg
      361 tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
      421 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
      481 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc ggcctgctcc
      541 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
      601 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
      661 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
      721 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
      781 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
      841 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
      901 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
      961 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
     1021 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
     1081 cagacgtgaa acccaacaga cccctgatcg taattctgag cactgtcgcg ctcgacgctg
     1141 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
     1201 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttgatg caatttgcct
     1261 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct
     1321 cgctggccgg cgctgctgtc gactacgcca tcatggcgac ggcgcctttc ctttgggttc
     1381 tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt
     1441 atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct
     1501 gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc
     1561 cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt
     1621 tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc
     1681 cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct
     1741 tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg
     1801 atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc
     1861 attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg
     1921 cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac
     1981 ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg
     2041 cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct
     2101 cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct
     2161 atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact
     2221 tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc
     2281 gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat
     2341 acgcgcccta ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag
     2401 gacgccgatg atttgaagcg cactcagcgt ctgatccaag aacaaccatc ctagcaacac
     2461 ggcggtcccc gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     2521 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     2581 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     2641 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     2701 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     2761 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
     2821 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
     2881 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
     2941 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
     3001 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
     3061 taaacccgcc ggcaacgccc gcagcatcgc gaccgtcatc ggtgagaagt tcggcaggta
     3121 ttgggtggtg acaatgtagg tgctgcccca aatggcaggt gctatcgctg tgaacaataa
     3181 atcggggcag acggcgcttc cagcgtgacg accatgccga gcacg
//
LOCUS       DACADA010000495         3005 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.299, whole genome shotgun sequence.
ACCESSION   DACADA010000495 DACADA010000000
VERSION     DACADA010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.299"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            <1..174
                     /locus_tag="ISB17_005116"
     CDS             <1..174
                     /locus_tag="ISB17_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAP1982916.1"
                     /translation="SIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFA
                     AGKYDITRPKAAG"
     gene            complement(381..458)
                     /locus_tag="ISB17_005117"
                     /pseudo
     CDS             complement(381..458)
                     /locus_tag="ISB17_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            482..1297
                     /gene="sul2"
                     /locus_tag="ISB17_005118"
     CDS             482..1297
                     /gene="sul2"
                     /locus_tag="ISB17_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1982917.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1358..2161
                     /locus_tag="ISB17_005119"
                     /note="aph(3'')-Ib"
     CDS             1358..2161
                     /locus_tag="ISB17_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP1982918.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2161..2997
                     /locus_tag="ISB17_005120"
     CDS             2161..2997
                     /locus_tag="ISB17_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAP1982919.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          600 a          835 c          859 g          711 t
ORIGIN      
        1 tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc
       61 aagcgccgcc agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta
      121 accgagttcg cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc
      181 ccactctatt gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta
      241 attggtaata ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt
      301 gcctactgag cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt
      361 atgctcttct gctcctgcag ctaatggatc accgcaaaca ggttactcgc ctggggattc
      421 cctttcgacc cgagcatccg tatgatactc atgctcgatt attattatta tagaagcccc
      481 catgaataaa tcgctcatca ttttcggcat cgtcaacata acctcggaca gtttctccga
      541 tggaggccgg tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc tgatggccga
      601 gggggcagat gtgatcgacc tcggtccggc atccagcaat cccgacgccg cgcctgtttc
      661 gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat
      721 tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt
      781 ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc aattggcgaa
      841 atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag atcggcgcga
      901 ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc
      961 gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca tggggttttt
     1021 tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg aattgcggct
     1081 gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac
     1141 aggccgtggt ccgggggatg tcggggccgc gacactcgct gcagagcttg ccgccgccgc
     1201 aggtggagct gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt
     1261 attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct
     1321 atattcgcgc ttcatcagaa aactgaagga acctccattg aatcgaacta atattttttt
     1381 tggtgaatcg cattctgact ggttgcctgt cagaggcgga gaatctggtg attttgtttt
     1441 tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct
     1501 cgctggagag cgtgaccgcc tcatttggct caaaggtcga ggtgtggctt gccccgaggt
     1561 catcaactgg caggaggaac aggagggtgc atgcttggtg ataacggcaa ttccgggagt
     1621 accggcggct gatctgtctg gagcggattt gctcaaagcg tggccgtcaa tggggcagca
     1681 acttggcgct gttcacagcc tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg
     1741 aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc
     1801 ggacgaggac aagagtacgc cgcagctcga tcttttggct cgtgtcgaac gagagctacc
     1861 ggtgcggctc gaccaagagc gcaccgatat ggttgtttgc catggtgatc cctgcatgcc
     1921 gaacttcatg gtggacccta aaactcttca atgcacgggt ctgatcgacc ttgggcggct
     1981 cggaacagca gatcgctatg ccgatttggc actcatgatt gctaacgccg aagagaactg
     2041 ggcagcgcca gatgaagcag agcgcgcctt cgctgtccta ttcaatgtat tggggatcga
     2101 agcccccgac cgcgaacgcc ttgccttcta tctgcgattg gaccctctga cttggggttg
     2161 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
     2221 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
     2281 aagggattga aacctataaa agacattgct gatgaactgc gcggggccga ctatctggta
     2341 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
     2401 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
     2461 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
     2521 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
     2581 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
     2641 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
     2701 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
     2761 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
     2821 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
     2881 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
     2941 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
     3001 tcaag
//
LOCUS       DACADA010000496         1117 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-16-3
            SAMN10735155-rid10686823.guided.375, whole genome shotgun sequence.
ACCESSION   DACADA010000496 DACADA010000000
VERSION     DACADA010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735155
            Sequence Read Archive: SRR8436646
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851662.1
            Genome Coverage       :: 222x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 47 of 116
            Pseudo Genes (incomplete)         :: 67 of 116
            Pseudo Genes (internal stop)      :: 21 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735155-rid10686823.guided.375"
                     /isolate="E02-16-3"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2013-04-13"
     gene            126..941
                     /locus_tag="ISB17_005121"
     CDS             126..941
                     /locus_tag="ISB17_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP1982920.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          298 a          231 c          257 g          331 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccct ctgatgttac attgcacaag
       61 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      121 gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
      181 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      241 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      301 gtagcgttgc caatgatgtt acagatgaaa tggtcagact aaactggctg acggaattta
      361 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
      421 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
      481 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
      541 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
      601 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
      661 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
      721 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
      781 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
      841 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
      901 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
      961 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcttcccta ccctcatggc
     1021 cgcctcccag gaatcgaccc aggtgcttat cgtggacata atcctttact cataaattgg
     1081 cactgttgca aagttagcga tgaggcagcc ttttgtc
//
